LOCUS       DBNHOX010000001         2865 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_642850, whole genome shotgun sequence.
ACCESSION   DBNHOX010000001 DBNHOX010000000
VERSION     DBNHOX010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_642850"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="AC16UZ_00005"
     CDS             <1..425
                     /locus_tag="AC16UZ_00005"
                     /inference="COORDINATES: protein motif:HMM:NF028497.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HIR6245086.1"
                     /translation="FGDAVSLDALEPAGPDGSLVSHLTLARDDKTPVPESRSAWQAGW
                     LPAGFTMAAADIRRTPSSLKSIDTMMYSDGLAAFSVFIEDMPPAGAASMVSRKGATVA
                     VTHLVANAEDEHHLVTLVGELPTDTAQRIARSITFRQD"
     gene            429..848
                     /locus_tag="AC16UZ_00010"
     CDS             429..848
                     /locus_tag="AC16UZ_00010"
                     /inference="COORDINATES: protein motif:HMM:NF016160.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HIR6245087.1"
                     /translation="MTTDLLTCRATVVASGPDGVEVGVMSPCGGCKAGCGSASRTGAR
                     MTLSSRAAVSGAPAAGTPVELAVSRSGLTKACALVFGIPIAGWLAATALVDAYWGEAV
                     ASAAGLGMLAWLLFAVRAFRPALRRWANIELIPLSRE"
     gene            876..2264
                     /locus_tag="AC16UZ_00015"
     CDS             876..2264
                     /locus_tag="AC16UZ_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HIR6245088.1"
                     /translation="MRNMLRFGYLILLLAWVEAGFGAQLPDFVDMVAKNSPAVVNIST
                     VQSPEAKSGRPRSRNDEFEEFFRRFFGPDGSRAPYPRPRSLGSGFVISKDGFVLTNNH
                     VVEGADEILVRFNDRREMNATLVGADPRSDLALLKVEAENLPTVKLGKSKDLDVGEWV
                     LAIGSPFGFDHSATAGIVSAKGRSLPTEDNENYVPFIQTDVAINPGNSGGPLFNLDGE
                     VVGINSQIYSNSGGFMGVSFAIPIDVAMEVVEQLKESGRVARGYLGVVIQEVSRELAE
                     SFGLERPHGALVTRVLEGSPAEGGGIREGDVIVAFNGEPIDRSAELPHLVGRAKVGAE
                     AALELVREGKSMRIELVIGELADRGPGSMTSGRSGSGGSERLGLYVEPVPEAAMERLG
                     ISGGVRVVEVSGAAEEAGLRPGDIITRMNNREVADPASLREIADELPSGRSVPVLVIR
                     GQVPTFLPLRVP"
     gene            2343..>2865
                     /locus_tag="AC16UZ_00020"
     CDS             2343..>2865
                     /locus_tag="AC16UZ_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR6245089.1"
                     /translation="MSSVASIRNFSIIAHIDHGKSTLADRLIQLCGGLADREMEEQVL
                     DSMDLERERGITIKAHSVTLSYKALDGIDYKLNFIDTPGHVDFSYEVSRSLAACEGAL
                     LVVDAAQGVEAQSVANCYTAIDQGLEVLPVLNKIDLPQAEPERVAAEIEDIIGLSAHD
                     VLCVSAKTGSGVPE"
BASE COUNT          481 a          871 c          974 g          539 t
ORIGIN      
        1 tcttcggcga cgcggtcagc ctggacgccc tcgagcctgc tggaccggac ggctccctgg
       61 tttcccacct gacccttgcg cgggacgaca agacgccggt gccggagtcc cgcagtgcct
      121 ggcaggcagg gtggctgcca gccgggttca ccatggccgc ggcggacatc cgtcgcacgc
      181 ccagttctct gaaatctatc gacaccatga tgtattcgga cgggttggcg gcattctccg
      241 tattcatcga ggacatgccg ccagcgggcg ccgccagcat ggtttcccgt aagggcgcca
      301 cggtggcggt cacgcatctg gtggcgaatg cggaagacga acatcacctg gtgacgctgg
      361 tgggtgagtt gcccacggac accgcgcaga gaattgcccg gtccatcacg ttccggcagg
      421 actgattgat gacgacggac ctgctcacct gccgtgccac cgtggtggcg agcggcccgg
      481 atggggtcga ggtgggggtg atgtcgccct gcggtggctg taaagccggc tgcgggagcg
      541 cgtccaggac cggcgcgagg atgaccctgt cttcccgggc cgctgtgagc ggtgccccgg
      601 cggccggcac gccggtcgag cttgccgtgt cccgcagcgg tctgacgaag gcctgtgccc
      661 tggttttcgg tatcccgatc gcgggctggc tggccgccac ggcgctggtc gacgcttatt
      721 ggggcgaagc cgtggcctcg gcggcggggc ttggcatgct cgcctggttg ctgtttgcgg
      781 ttcgggcgtt tcgtcctgcg ttgcggcgct gggccaatat tgagttaatc ccgctttcac
      841 gcgagtaatg gcgccgcgag gcgaccgatg gttgtatgag aaatatgctt agatttggtt
      901 acctgattct gctccttgcc tgggtagagg cgggctttgg ggcgcagctg cctgatttcg
      961 tggacatggt cgccaagaac tcgccggccg ttgtgaacat aagcaccgtc cagagccctg
     1021 aggccaaatc cgggcgtccc cgctcgcgaa acgatgagtt cgaagagttc ttccggcgat
     1081 tcttcggccc ggatggcagc agggcgccct acccgaggcc ccgttctttg ggttccggct
     1141 tcgtcatctc caaggatggc ttcgtgctga cgaacaacca cgtcgtggaa ggcgccgacg
     1201 agattctcgt gcgcttcaat gaccggcgag aaatgaacgc caccctcgtg ggggcagacc
     1261 ctcgttcgga ccttgccctg ctcaaggtcg aagcggaaaa cctgccgacg gttaagctcg
     1321 gcaagtcgaa ggatctggac gtgggtgagt gggtgctcgc catcgggtcg ccgttcggct
     1381 tcgaccactc ggcaaccgcc ggcatcgtca gcgccaaggg gcgcagtctc cccacggagg
     1441 acaacgagaa ctacgtgccc ttcatccaga ccgatgtggc catcaacccg ggcaattccg
     1501 gtggtccgct gttcaatctg gacggcgagg tcgtcgggat caactcccag atctacagca
     1561 actccggcgg gttcatgggc gtctccttcg ccattcccat cgacgttgcc atggaggtgg
     1621 tcgagcagct gaaggagtcc gggcgcgttg cgcgcggcta cctgggcgtg gtcattcagg
     1681 aggtcagccg cgagctggcc gagagcttcg ggctcgagcg tccccacggt gcgctggtta
     1741 cccgtgttct cgaaggcagc cccgcagagg gtggcgggat tcgtgaagga gacgtcatcg
     1801 tggctttcaa tggcgagcct atcgaccgct cggcggagtt gccgcatctc gtcgggcgcg
     1861 ccaaggttgg tgccgaagca gcgctagaac tggtccggga agggaaatcc atgagaatcg
     1921 agctggtcat tggcgagctg gccgatcggg gtccgggctc catgacctcg ggcaggagcg
     1981 gcagcggcgg atcggaacgt cttggacttt acgtggagcc ggtgcctgaa gcggccatgg
     2041 agcggcttgg catcagcggt ggcgttcgcg tcgtggaggt cagcggtgcc gcggaagagg
     2101 ccggcctgcg gcctggggac atcatcaccc gtatgaacaa tcgggaggtc gcagacccgg
     2161 caagcctacg cgagatcgcc gacgagctgc cctcggggag gtccgtgccg gtgctcgtca
     2221 tcaggggaca ggtgccgaca ttcctgcctc tgcgggtgcc atgaaggcga gagccgattg
     2281 gcccggcatc tcccgataag ctacactcgc gcgccttcat cgggcacctg gttcgacagg
     2341 gtttgagcag tgtcgcttcc atccgtaatt tttcgatcat cgcgcacatc gaccacggca
     2401 agtcgaccct ggccgatcgc ctgatccagc tgtgcggcgg tcttgccgac cgggaaatgg
     2461 aagagcaggt gctcgactcc atggatttgg aacgcgagcg cgggatcacc atcaaggcac
     2521 acagcgtcac gctgtcctac aaggctctgg atggcatcga ctacaagctg aacttcatcg
     2581 acacgcccgg ccacgtggac ttcagctacg aagtttcccg atcgcttgca gcctgcgaag
     2641 gcgcgctgct cgtcgtcgat gccgcccagg gggtggaagc gcagtcggtt gccaactgct
     2701 acacggcgat cgaccagggc ctggaggtcc tgccggtact caacaagatt gacctccccc
     2761 aggcagagcc cgagagggtt gctgcggaga tcgaggacat catcgggtta tctgcgcacg
     2821 acgtcctctg tgtcagcgcc aagaccggct cgggagtgcc cgaac
//
LOCUS       DBNHOX010000002        10638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1349977, whole genome shotgun sequence.
ACCESSION   DBNHOX010000002 DBNHOX010000000
VERSION     DBNHOX010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10638
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1349977"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="AC16UZ_00025"
     CDS             complement(<1..645)
                     /locus_tag="AC16UZ_00025"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6245090.1"
                     /translation="MTTRIRGVFFDLGGTLFSYRNMGRSTGPALLEAAARMGITGDPR
                     SIGKAYGLASREVNGAYAERDFYLHRDLFRDTFVRFVEQMEGQFDEAAYAWFRARQHE
                     LVLSGLELKPDCTETLSHLKSEGLYLSIVSNIDDDMLDPLVEREGLHDYLHHWTSSEA
                     AESCKPHRRFFEISLEKSGLAPGEVLFVGDSPEHDVQGAYEVGMNTVLIREEGLE"
     gene            complement(706..2352)
                     /locus_tag="AC16UZ_00030"
     CDS             complement(706..2352)
                     /locus_tag="AC16UZ_00030"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HIR6245091.1"
                     /translation="MQHTPLLMSNILDRGAKVAPDVEIVTATENGTRRQTYAEIRDRA
                     HQLAHALRDAGIGVGDRVATFMWNGSRHLEAYHAIGGMGAVLHTLNIRLSAKDLEYII
                     NHAEDRVIIADADVLPLLEPLKGRIPSVERIVVATEDGFEGWSTEVAEAVDYEEFIAG
                     KPTRFDWPRIDENSALGLCYTSGTTGNPKGVEYEHRSQYLHTLTQCMTDSMGLSATDC
                     VLGIVPMFHAMGWGMPWSCLMLGCKQVMPHRFMDTSRLLDLLVSEGVTMSAGVPTIWQ
                     GIKALVEADPERYDLSRLARMACGGSAPPPSLIRWYWDTLGVEMIQGWGMTETSPLAT
                     LSRRVMKRSHLELSEDDQFRNVAKAGQLMPGLELDIFDEDFNRLPHDGETVGEILIRG
                     PWICSEYYHDPQPEKFHDGWLITGDVGKIDSEEYLIISDRSKDLVKSGGEWISSVDLE
                     NHIVSLDGVEQACVVAQPHPKWDERPVALVVLGDGADVSLETVLAHCSQAFAKWQLPD
                     DVLYVDSIPLTSTGKMDKKIVRADLEAQGYVLPDLREKAG"
     gene            complement(2425..4002)
                     /locus_tag="AC16UZ_00035"
     CDS             complement(2425..4002)
                     /locus_tag="AC16UZ_00035"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0032787 - monocarboxylic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6245092.1"
                     /translation="MTSSMMRLTQGIHRALSLYPRRRAVVSAEGEITWEQFVDRVARL
                     AGALRELGVGPGDRVAMLALNSPRYVEYYFAVLWAGGVLAPVNCRWAAPEKAHCLNDS
                     GAKVLLADAAHLPEALELLETCGSVSHLVSCGEVEGSGTAALGYESLVSASLPVEDAE
                     RGGADLAALFYTGGTTGRAKGVMLSHDNFIANSMTALVNLGISEHSVHLHVAPLFHLA
                     GGSRLFTLTVAGGTHAVIPRFDAAEFLGAIERFGVTVTVIVPTMLNTLLQHPNFEAFD
                     LSSLDLLCYGASPMPESLLREAMERLPGARFLQSYGMTELSPVATMLEPQFHTFEGPL
                     AGKIRSAGRAVFNADVAIMSPDDLPLASGEVGEICVRGPMVMQGYWGQPELTAQALRG
                     GWMHTGDAGYLDDDGFLFLVDRVKDMIITGGENVYSAAVENVLYLHPGVHECAVIGVP
                     SDAWGEAVHAIIVPRGGARLDEAAVIEHCRAYLAGYECPKTVEFVDAELPKSGAGKIL
                     KAELRKTYWQGQARNIH"
     gene            complement(4058..5233)
                     /locus_tag="AC16UZ_00040"
     CDS             complement(4058..5233)
                     /locus_tag="AC16UZ_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6245093.1"
                     /translation="MSGPLTGVRVLDLTSMVSGPVATMLLGDQGADVIKVEPLTGDLV
                     RYMGPNRDGLTSGFISSNRNKRSIAVDLKQEAGMDVVKRLAATSDVFVQNFRPGAIER
                     MGLGEDVVREIRPDIVYVSISGFGETGPYAHKRVYDPVIQALSGLAAIQRDRETGRPK
                     MVRTIIPDKTTALTAAQAITAALFARERSGEGQHVKLAMLDTMVAYLWPEGMAGFTFI
                     GKEVKAARAQFAQDLIFATTDGFITAGAVSDAEWQGMCRALEREEWIDDERFNTPNGR
                     IVNVQERLRLTAEVLETRSSAEWLARLDAEGVPCAPVLDRHEVFEHEQVRINEMVEEL
                     DHPVAGRIRQPRPAARFDRTPAGIHRHAPMLGEHTDELLQEMGLDADALRAAGVIGG"
     gene            complement(5243..6856)
                     /locus_tag="AC16UZ_00045"
     CDS             complement(5243..6856)
                     /locus_tag="AC16UZ_00045"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6245094.1"
                     /translation="MDFHFASAWEAIADTVPDRTALICEGVTRTWADYDGRATRVAGL
                     LQAHGLGADTKVGLYLHNCNEYLEAQYGVFKLSGCPINVNYRYKAEELVYLLDNADAE
                     AVFYQGCYAPRIEEIRDQLPRVKLYVQVDDGTEDLLAGSVDYESSLDAAEPMARQKRD
                     PASVYMLYTGGTTGMPKGVMYPGGEFCYLLTAMGAGGRGLVPPQNLEALREYVAGIED
                     PPVTVVACPLMHGTGMWLGSLAPLLTGGTVITTPQLGLDPDLIWTLVGSHRATDLVVV
                     GDAFARPLLAALDEAERRGEPYDLSSLRQIVSSGVMWSREVKEGLLRHRDMMLVDAMG
                     STEGSMGSSVSSRASGPTTAKFQLAEGVKVFTDEGREVEPGSGEIGKLATSGFVPLGY
                     YKDPVKSAETFREVDGVRYSFPGDYATVEADGSITLLGRGSVCINTAGEKVFPEEVEE
                     AVKRHPAVVDCLVVGIPDERFGERVVAVASFHDDQDVSEPELIEFARDHLAGYKLPKQ
                     VLRVPQVQRAPNGKADYKWAKSTALAELT"
     gene            7130..7345
                     /locus_tag="AC16UZ_00050"
     CDS             7130..7345
                     /locus_tag="AC16UZ_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245095.1"
                     /translation="MIIKDNAPAGPIARLSRITYPRASAANLMLLSVVIAAIGAIAYI
                     LHPDAAQPAPNTFHGAALLVSNWLLCG"
     gene            7373..7585
                     /locus_tag="AC16UZ_00055"
     CDS             7373..7585
                     /locus_tag="AC16UZ_00055"
                     /inference="COORDINATES: protein motif:HMM:NF015957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR6245096.1"
                     /translation="MANSNLNNVRRRLRLDPRNGWIAGACAGFANRLQIDPAIVRVGV
                     VIAALFFPKTVIACYLLAWLVLDEAR"
     gene            complement(7610..8503)
                     /locus_tag="AC16UZ_00060"
     CDS             complement(7610..8503)
                     /locus_tag="AC16UZ_00060"
                     /inference="COORDINATES: protein motif:HMM:NF039714.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HIR6245097.1"
                     /translation="MIERMVVRLFALLAVLVLGSGCSVQFAYNNLDRLVRWGVNDYVN
                     MNDAQRNLFNEEFARLHYWHRTEHMPLYADFFEAVPTTLSDGVDAQELLALENTMMGW
                     AKEIVETGAPLAVAIMQTMTDEQVAGLPARLEESNREIEKTELGKSVEASQARWREEV
                     EDIFTRFAGRLTPAQQEYLVAQSVRYIPERVLWAEYRRRWQRDLMKLLQARSEPAFAD
                     RFAELAANREAYFGAELTAIFDNNENLGRETAAWLLNHMAERQSVRMYTRFVELAIDF
                     RELADDAPNWLPPEEPCQITC"
     gene            complement(8496..9197)
                     /locus_tag="AC16UZ_00065"
     CDS             complement(8496..9197)
                     /locus_tag="AC16UZ_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase-related protein"
                     /protein_id="HIR6245098.1"
                     /translation="MRIGILQTGAVLEDIQPRFGDYPAMFVDLLSGDAHRRPSFSTYR
                     VREGELPEPAACDAYVITGSRHSVYEDHPWIVELAEFVGNTMAGGQKIVGICFGHQLI
                     AHYFGGRTEPASVGWGVGVHGVEIVSRERWMDPHQGSVSMLSSHKDQVVALPRGARRV
                     ASSAVCPNAAFAIGDVAMTWQGHPEFEKDYAETLMRRREALLGPETLAAGLASLDNEI
                     DRALLARWILNFIYD"
     gene            complement(9215..10591)
                     /locus_tag="AC16UZ_00070"
     CDS             complement(9215..10591)
                     /locus_tag="AC16UZ_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR6245099.1"
                     /translation="MNDGMELLDQKLARYRERGVSRVKLGLTDVDGVIRGKYVSLSKF
                     ASLMEKGGGFCDCVFGWDVHDQLYDAGTYTGWHTGFPDAQYRLVADSERWLSEEGCPF
                     FLGEFVAGDGGPHPLCPRTLLKTVLGRLGDRGLTLKSGFEYEFFVFAETPHSVRDKGY
                     RNLRPLTPGNFGYSLLRASGEADKFSGLMDFSQALDCDLEGLHCETGPGVWEAALQTA
                     LGEEAADRANIFKTFAKVYFNRLDCMASFMAKWSMDYPGQSGHFHLSLLDADGRNVFF
                     DDGQPSGMSDLQRQAVAGIERYLPEYLALIAPTINSYTRLVKGAWAPTASTWGIDNRT
                     VAIRVINGSESHQHIECRVPGADSNPYLVAAAVCAAALGGIEQALLPGDPVVGNSYDV
                     EDSLPEELRFPRDLAAAADRLEASDHARAMLGEAFVDHYVMSRRWECEEYRKNLNSWQ
                     LERYFEII"
BASE COUNT         1946 a         3591 c         3275 g         1826 t
ORIGIN      
        1 ctcgagaccc tcctcacgaa tgagaaccgt gttcatgccc acctcgtagg cgccttggac
       61 gtcgtgctcg ggggaatcgc cgacgaacag aacctcgcca ggcgcgaggc cgctcttctc
      121 caggctgatc tcgaagaatc gccggtgggg cttgcacgac tccgcagcct ctgagctcgt
      181 ccagtgatgc agatagtcgt gaagcccttc ccgctcgacg agcgggtcga gcatgtcgtc
      241 gtcgatgttg gagacgatgg agaggtacag cccttccgac ttcagatgcg aaagggtttc
      301 ggtgcagtcc ggcttgagct cgagcccgct cagcaccagc tcgtgctgcc gggcgcggaa
      361 ccaggcgtag gcggcctcgt cgaactgccc ctccatctgc tccacaaagc gcacgaaggt
      421 gtcgcggaag agatcccggt gcaggtagaa gtcccgctcg gcgtaagcgc cgttgacctc
      481 ccgggatgca agcccgtacg ccttgccgat gctgcgcgga tcgccagtaa tgcccatgcg
      541 cgcagccgcc tcgagcagcg cggggccggt ggagcggccc atgttgcgat agctgaacag
      601 cgtgccgccc aggtcgaaga acacgcccct gatgcgcgtc gtcatggcga aacgatgctc
      661 acgcgatgcg cggccatcgg ccaaaacgcc ggaatttcca accgatcacc ccgctttctc
      721 ccgcagatcg gggagcacgt aaccctgagc ctccaggtcc gcgcggacga ttttcttgtc
      781 catcttgccg gtggacgtca gcggaatgct gtcaacgtaa agcacgtcat cggggagctg
      841 ccactttgca aacgcctggc tgcagtgagc cagcacggtc tccaggctga cgtcggctcc
      901 gtcgccgagc accacgagcg ctaccggacg ctcatcccat ttcgggtgcg gctgcgccac
      961 cacgcaggcc tgctcgaccc cgtcgagaga gacgatgtgg ttctccaggt ccacggagga
     1021 gatccactcc ccgccgctct tgacgagatc cttggagcga tcgctgatga tcaggtactc
     1081 ctcgctgtcg atcttgccga cgtcgcccgt gatcagccag ccgtcatgaa acttctccgg
     1141 ctggggatcg tggtagtact ccgagcagat ccagggtcca cggatgagga tctcgccgac
     1201 ggtttcgcca tcgtggggca ggcggttgaa atcctcgtcg aagatgtcga gctccagacc
     1261 gggcatcagc tgtccggctt tggcgacgtt gcggaactga tcatcctcgc tgagctcgag
     1321 gtgggaacgc ttcatcaccc gccgcgacag cgtggccagc ggcgacgtct cggtcatgcc
     1381 ccatccctga atcatctcta ccccgagggt gtcccagtac cagcggatca gcgacggcgg
     1441 cggcgcggag ccgccgcagg ccatgcgagc caggcgggaa aggtcgtagc gctccgggtc
     1501 ggcctcgacc agcgctttga tgccctgcca gatggtcggc acgccggctg acatggtcac
     1561 gccctcggac accagcagat ccagcagcct cgacgtatcc atgaaccggt gcggcatgac
     1621 ctgcttgcag cccagcatga ggcacgacca gggcatgccc cagcccatgg cgtgaaacat
     1681 gggcacgatg ccgagcacgc aatccgtggc ggagaggccc atggagtcgg tcatgcactg
     1741 agtgagcgtg tgcagatact gagaacggtg ctcgtactcg acgcccttcg ggttgcccgt
     1801 ggtgccgctg gtgtagcaca ggccgagggc ggagttctcg tcgatccggg gccagtcgaa
     1861 acgggtcggc ttgccggcaa tgaattcctc gtagtcgacg gcctcggcca cctcggtgct
     1921 ccagccttcg aaaccatcct cggtggcgac cacgatccgc tccacgctgg gaatccgtcc
     1981 cttgagcggc tccagcagcg gcaacacgtc cgcatccgca atgatcacgc gatcctcggc
     2041 gtggttgatg atgtactcga ggtccttggc cgaaaggcgg atgttcagcg tatgcagcac
     2101 cgcgcccatg ccgccgatgg cgtgatacgc ctccagatga cgggacccgt tccacatgaa
     2161 agtcgccacg cgatctccca cgccgatgcc ggcatcacgc agggcgtggg cgagctggtg
     2221 ggcacgatcg cggatctcgg cataggtctg ccgccgcgtg ccgttttcgg tagcggtgac
     2281 gatctccacg tcgggcgcca ccttcgcgcc acgatccaga atattggaca tcagcaatgg
     2341 cgtatgctgc atcgccacgg ttcgccacct cccttagact ttcgttccgg tgaattcagc
     2401 gtgagggcag cgagtctatc atcctcagtg aatgttgcgc gcctgaccct gccagtaggt
     2461 cttgcgaagc tcggccttca ggatctttcc cgccccgctc ttcggcagct cggcgtcgac
     2521 gaactccacc gtcttcgggc actcgtagcc ggcaaggtag gcacggcagt gctcgatgac
     2581 ggccgcctcg tcgagccggg cgccaccgcg aggcacgatg atggcgtgaa cggcctcgcc
     2641 ccaggcatcg ctcgggaccc cgatcaccgc acactcgtgc actccggggt gcaggtacag
     2701 cacgttctcc acggccgcag agtagacgtt ctcgccgccc gtgatgatca tgtccttcac
     2761 ccgatcgacg agaaacagaa aaccatcgtc gtcgaggtaa ccggcgtctc ccgtgtgcat
     2821 ccagccaccg cgcagcgcct gagcggtgag ctccggctgg ccccagtatc cctgcatgac
     2881 catgggtccg cgaacacaga tctctcccac ctctcccgac gcaagcggaa ggtcgtcggg
     2941 gctcatgatg gccacgtccg cgttgaaaac ggcacggccg gccgaacgga tcttccccgc
     3001 cagcggaccc tcgaaggtat ggaactgcgg ctcgagcatc gtagccacag gagacagctc
     3061 ggtcatgccg taggactgca ggaagcgggc tcccggcagc cgctccatgg cttcacgcag
     3121 aaggctctcg ggcatgggcg aggcgccgta gcagagaaga tccagggacg aaagatcgaa
     3181 ggcttcgaag ttcggatgct gcagcagcgt gttgagcatg gtcgggacga tgacggtgac
     3241 ggtcaccccg aagcgctcga tcgcccccag gaactcggcg gcgtcgaagc gggggatcac
     3301 cgcatgggtc cctccggcca cggtcagggt gaacagccgg gagccgccgg ccagatggaa
     3361 aagcggcgca acatgaaggt ggacgctgtg ctccgagatg cccagattca ccagagccgt
     3421 catcgagttg gcgatgaagt tgtcgtggct gagcatcacg cctttggcac gcccggtggt
     3481 cccgcccgtg tagaacagcg ctgccagatc cgctccgccc cgctcggcgt cctcgaccgg
     3541 gagcgacgcc gaaaccagcg actcgtagcc cagcgccgcg gttcccgagc cctccacctc
     3601 tccgcaggag accaaatgag agacgctccc gcaggtttcg agcagctcga gcgcttccgg
     3661 gaggtgggcc gcgtccgcaa gcagaacctt cgcgccgctg tcgttgaggc agtgcgcctt
     3721 ctctggcgcc gcccagcggc agttgaccgg agcaagaacg cccccggccc atagcacggc
     3781 gaagtagtac tccacatagc gcggcgagtt gagcgcaagc atggcgactc gatcgcccgg
     3841 acccacaccc agctctctca gcgcgcctgc caggcgcgcc acgcgatcga caaactgctc
     3901 ccaggtgatc tcgccctccg ccgaaaccac cgcccgccgg cgagggtaga ggcttagggc
     3961 ccggtgaatg ccctgggtga ggcgcatcat tgaggaagtc atggcggcgc gggattctac
     4021 aggcagctca ggcgacgagc gagccagccc cggcggatca gccgccgatc accccggcgg
     4081 ccctgagcgc gtccgcgtcc agacccattt cctgcagcag ctcgtcggta tgctcgccga
     4141 gcatcggcgc gtgccggtgg atgccggccg gcgtcctgtc gaaccgcgcc gccggtctcg
     4201 gctgacgaat gcggccggcc accgggtggt ccagctcttc gaccatctcg ttgatcctca
     4261 cctgctcgtg ctcgaagacc tcgtggcgat ccagcacggg ggcgcaggga acgccttcgg
     4321 catcgagtcg ggccagccac tccgcgctgc tgcgcgtctc cagcacctcg gcagtcaggc
     4381 gcagccgctc ctgcacgttg acgatccggc cgttgggcgt gttgaaccgc tcgtcatcaa
     4441 tccactcctc acgctctagc gcgcggcaca taccctgcca ctccgcatcg gacaccgcgc
     4501 ctgccgtgat gaagccgtcg gtggtggcaa agatgaggtc ctgagcgaac tgggcccgcg
     4561 cagctttcac ttccttgccg atgaacgtga agcccgccat gccctccggc cagagatagg
     4621 ccaccatggt gtcgagcatg gctagcttca catgctgacc ttcgccgctg cgctcccggg
     4681 cgaagagggc cgcggtgatg gcctgggccg cggtaagcgc cgtcgtcttg tccggaatga
     4741 tggtgcgcac cattttcggc cttccggttt cccggtcgcg ttggatcgcg gcaagaccgg
     4801 aaagcgcctg aatcaccggg tcgtacaccc gcttgtgcgc gtaagggccc gtctctccga
     4861 atccgctgat ggacacgtag acgatgtccg ggcggatctc ccgcaccacg tcctctccca
     4921 gacccatgcg ctcgatggcc ccaggccgga agttctgaac gaagacgtcg gaagtggcag
     4981 ccaggcgctt gaccacgtcc atgccggcct cctgcttgag gtccaccgcg atgctccgct
     5041 tgttgcggtt cgagctgatg aacccggacg tgaggccatc ccggttgggc cccatgtagc
     5101 ggacgagatc gcccgtcaac ggctcgacct tgatgacgtc ggcaccctga tcaccgagca
     5161 gcatggtggc caccgggccg gaaaccatgc tggtcaggtc gaggacccgg actccagtta
     5221 gcggtccgct catatcaacc tctcaagtca gttccgcgag cgccgtgctt ttcgcccact
     5281 tgtagtccgc cttgccgttg ggcgcgcgct gcacttgcgg cacccgcaga acctgcttgg
     5341 gaagcttgta gcctgcaagg tggtcgcggg caaactcgat caattcgggc tcggatacgt
     5401 cctggtcgtc gtgaaaggac gcgaccgcca ccacgcgctc gccaaaacgc tcatcgggaa
     5461 tgccgacgac caggcaatcg acgacggccg gatggcgctt gacggcctct tcgacctcct
     5521 cggggaacac cttctcgccg gccgtgttga tgcagacgct gccgcgcccg agcagcgtga
     5581 tgctgccgtc cgcttccacg gtggcgtagt cgccgggaaa gctgtaccga acgccgtcca
     5641 cctcgcggaa cgtttccgca gatttgaccg gatccttgta gtagccgagg ggcacgaagc
     5701 cgctggtggc gagcttaccg atctcgcccg aacccggctc aacctcgcgg ccctcgtcag
     5761 tgaagacttt caccccttcg gcgagctgaa acttggccgt ggtcggcccc gacgcccggg
     5821 agctgacgga gctgcccatg cttccttccg tcgagcccat ggcgtccacg agcatcatgt
     5881 cccgatgacg cagcagccct tccttgacct ctctgctcca catcaccccg ctggagacga
     5941 tctgccgcag ggaactgagg tcgtaaggct cgccgcgacg ctcggcctcg tcgagcgcgg
     6001 ccagcagcgg acgcgcaaag gcgtccccga caacaacgag atccgtcgcc cggtggctgc
     6061 ccaccagggt ccatatcaga tcgggatcca gccccagctg cggcgtcgtg atcaccgtgc
     6121 cgccggtgag cagcggcgcg aggctgccca gccacatgcc cgtgccgtgc atcagcgggc
     6181 aggcgaccac cgtaacgggc ggatcctcta tacccgccac gtattcccgc agggcctcga
     6241 ggttctgcgg gggcaccagg ccgcgccccc cggcgcccat ggcggtgagc aggtagcaga
     6301 actcgccgcc ggggtacatg acgcccttgg gcatgcccgt ggtgcccccc gtgtagagca
     6361 tgtaaacgct cgccggatcc cgcttctgcc tcgccatggg ctcggcggcg tcgagcgagc
     6421 tttcgtagtc aaccgacccc gccagcagat cctcggtccc gtcatccacc tgcacataga
     6481 gcttcacgcg gggaagctga tcgcggatct cttcgatgcg cggggcgtaa cacccctggt
     6541 aaaagaccgc ctccgcgtcc gcgttgtcca gcaggtaaac cagctcctcc gccttgtagc
     6601 ggtagttcac gttgatcgga cagccgctga gcttgaagac gccgtactga gcttcgagat
     6661 actcgttgca gttgtggaga tacaggccca ccttcgtatc cgcgcccagg ccgtgggcct
     6721 gaagcagacc ggccacccgc gtagcacggc cgtcgtaatc cgcccaggtg cgggtcacgc
     6781 cctcgcagat gagggccgtc cggtccggaa ccgtgtcggc gatggcctcc caggccgatg
     6841 cgaaatgaaa atccatggca gacgccctcc ctgtagaaga aatccggatt ggcaatgggt
     6901 cgtccgtcct gacatcgcca ggccgaccga attcgccaac tgttcgcaga aatcgccaac
     6961 accacaggcc cgcccgtcgc cccttccacc cggcgaggcg gcattaagcc tggagatcgg
     7021 ggttgttcgg agttggcatg gcatttgaat attcagtaac gaggccaacg gaaacgacct
     7081 ggcttcagct gcaaatgaac gcgagcagac aatgaaacgg caaaaccaga tgataatcaa
     7141 agacaatgca cccgcaggcc cgatcgcgcg cctgtctagg atcacctatc cccgggcttc
     7201 ggcggcaaac ctgatgctgc tgagcgtggt gatcgccgcc atcggcgcga ttgcctacat
     7261 cctgcacccc gatgccgccc agccggcgcc gaacaccttc cacggcgcgg ccctgctggt
     7321 cagcaactgg ctgctgtgcg gctgacgaca gaaagactcc ggagagatcc cagtggcaaa
     7381 ttcgaacttg aacaacgtcc gcagacgact gcgtctcgac ccgcgcaacg gctggattgc
     7441 cggcgcctgc gccggcttcg ccaatcggtt acagatcgac cctgccatcg tgcgggtcgg
     7501 tgtggtgatc gccgccctgt tcttccccaa gacggtgatc gcctgttacc tgctggcatg
     7561 gctggtgctg gacgaagctc gctgagccca ccggcggtga gcccttccat cagcaggtga
     7621 tctggcaggg ctcctccggc ggcagccagt ttggcgcgtc gtcggccagc tcgcgaaagt
     7681 ctatagccag ctccacgaaa cgcgtgtaca tgcgcaccga ctgccgctcg gccatgtggt
     7741 tgagaagcca ggcggcggtc tcgcgcccca ggttctcgtt gttgtcgaag atggcggtca
     7801 gctcggcccc gaaataggcc tcgcgattag cggccagctc cgcgaagcga tccgcaaagg
     7861 ccggttcgga gcgggcctgg agcagcttca tgaggtcacg ctgccagcgc cgccgatact
     7921 ccgcccagag aacccgctcg ggaatgtagc gaaccgactg agccaccaga tactcctgct
     7981 gcgccggagt caggcgaccg gcaaagcggg tgaagatgtc ctccacctct tcgcgccagc
     8041 gggcctggga cgcttcgacg ctcttgccga gctcggtctt ctcgatctcc cggttgctct
     8101 cctccagccg tgccgggagg ccggccacct gctcatcggt catggtctgc atgatggcga
     8161 ccgcgagggg cgcgcccgtc tcgacgatct ctttcgccca gcccatcatg gtgttttcca
     8221 gcgcgagcag ttcctgagca tcgacgccgt ccgaaagcgt cgtcggcacg gcctcgaaga
     8281 agtccgcata gagcggcata tgctccgtgc gatgccagta atgaagccgg gcgaattcct
     8341 cgttgaacag gttccgctga gcgtcgttca tgttcacgta atcgttcacg ccccaacgca
     8401 ccagccggtc gaggttgttg taggcaaact gaacgctgca gccggaacca agcaccagca
     8461 ccgcgagcag cgcaaacaga cggacgacca tgcgctcaat catagatgaa gttcagaatc
     8521 cagcgcgcca gcagcgcgcg gtcgatctcg ttgtcgagcg aggccaaacc ggccgcaagg
     8581 gtctcgggcc ccagaagcgc ttcgcggcgg cgcatcaacg tttccgcata gtccttctcg
     8641 aactccgggt ggccctgcca cgtcatggcc acatcgccga tggcaaacgc cgcgttcggg
     8701 cagacggcgc tggaggccac gcggcgcgcg ccgcgcggca gggccaccac ctgatccttg
     8761 tgactcgaca gcatgctcac cgatccctgg tgcgggtcca tccaacgttc cctgctcacg
     8821 atctccaccc catggacgcc tacaccccaa cccaccgagg ccggctcggt gcggccgccg
     8881 aagtaatggg caatgagctg gtggccaaag cagatcccca cgatcttttg tccgccggcc
     8941 atggtgttac cgacgaactc cgccagctcg acgatccagg gatgatcctc gtaaacgctg
     9001 tgtcggctgc ccgtgatgac gtaggcgtcg caggccgccg gctcaggaag ctcgccttcc
     9061 cgcacgcggt aggtcgagaa actcgggcgc cgatgcgcat ctccgctgag cagatccacg
     9121 aacatggccg ggtagtcgcc aaaccggggc tgaatatcct ccagaacggc gccggtctgc
     9181 agaatgccga tgcgcatcag gcaatgtttc cccgtcagat gatctcgaag tagcgttcca
     9241 gctgccagct gttgaggttc ttgcggtact cttcgcactc ccagcgccgg gacatgacgt
     9301 agtggtcgac gaaggcctcg ccgagcatcg ccctggcatg gtcggaagcc tccagacgat
     9361 cggccgcggc cgccaggtcg cggggaaacc taagctcctc gggcagagag tcctccacgt
     9421 cgtaggaatt tccgaccacg ggatctccgg gcaggagggc ctgctcgata ccccccagcg
     9481 ccgcggcgca cacggcggca gcgacgagat aggggttaga gtcggcgccc gggacacggc
     9541 actcgatgtg ctggtgcgat tcagatccat tgatgacgcg aatggccacg gtgcggttat
     9601 cgatgcccca ggtcgaagcg gtgggcgccc aggctccttt cacgagtcgg gtgtagctgt
     9661 ttatggtggg agcgatcaac gcgaggtact ccggcagata ccgctctatg ccggccaccg
     9721 cctgacgctg cagatctgac atgcccgacg gctggccgtc gtcgaagaaa acgtttcgcc
     9781 cgtcggcatc gagcaagctc aaatgaaagt gaccgctctg gcccggatag tccatggacc
     9841 acttcgccat gaagctcgcc atgcagtcca gccggttgaa gtagactttc gcaaaggtct
     9901 tgaagatatt ggcacgatcg gccgcttcct cgccgagcgc ggtctgcaat gccgcttccc
     9961 acaccccggg acccgtttcg cagtgaaggc cttccagatc gcagtccagc gcctgggaaa
    10021 agtccatgag accggagaac ttgtcggctt cgccgctggc cctcaacagg gagtaaccga
    10081 agttgcccgg ggtcagcggg cgcaggttcc gatatccctt gtcccgcacc gaatgcggcg
    10141 tctcggcaaa gacgaaaaac tcgtactcga agccagactt gagggtgagc ccacggtcac
    10201 cgagcctgcc cagcaccgtc ttgagaagcg ttcgcgggca caggggatgg ggaccaccgt
    10261 caccggccac gaactcgccc aggaaaaacg ggcacccctc ctccgaaagc cagcgctcgc
    10321 tgtcggcaac cagccgatac tgggcatcag gaaaacccgt atgccacccc gtataggtac
    10381 cggcgtcgta gagctggtcg tgcacgtccc atccgaagac gcagtcacag aagcccccac
    10441 ccttctccat cagcgaggcg aacttggaca aactgacgta cttcccgcgg atcacgccat
    10501 cgacgtcggt cagccccagc ttgacacggc tgacaccccg ttcacgatac cgcgcgagct
    10561 tctgatccag cagttccata ccgtcgttca agtaagatct cctcgtttgg cctcaacatg
    10621 tggagcagac tttacctc
//
LOCUS       DBNHOX010000003         6113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_128649, whole genome shotgun sequence.
ACCESSION   DBNHOX010000003 DBNHOX010000000
VERSION     DBNHOX010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_128649"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="AC16UZ_00075"
     CDS             complement(<1..275)
                     /locus_tag="AC16UZ_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245100.1"
                     /translation="MLGKLRANLPLRFVQHRDGLFLGADGVLESAGFGVRGCQGVQIA
                     WYALAGDSALGTLDGQVAVAKVGFLVGSKQPRLVVVGLWVVLSGGPG"
     gene            408..614
                     /locus_tag="AC16UZ_00080"
     CDS             408..614
                     /locus_tag="AC16UZ_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245101.1"
                     /translation="MIQEIVGAKGRDFRPGDIASVLRERGQPLGAWEIRGQLSILEAE
                     GLIRLVPESGSWEMASSENHRAVS"
     gene            684..944
                     /locus_tag="AC16UZ_00085"
     CDS             684..944
                     /locus_tag="AC16UZ_00085"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR6245102.1"
                     /translation="MAKTSETPDDLSDELDFESALEDLESLVESMESGEMSLEESLRA
                     FERGIKLTRHCQSALKAAELKVRMLTENDELVALDADDPPPE"
     gene            941..1822
                     /locus_tag="AC16UZ_00090"
     CDS             941..1822
                     /locus_tag="AC16UZ_00090"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR6245103.1"
                     /translation="MTPELDELRALINDALDHHLPSSSAIAQPLVDAMRYTTLGGGKR
                     IRPMLVCATCTGLGGALEDALAPACAVEFIHAYSLIHDDLPSMDDDELRHGLPSSHIK
                     FGEATAVLAGDALQALAFETLSRAPSGSDAQRLRAVQLLARAAGPQGMVGGQSYDLES
                     ERKKLSLSQLQTLHAAKTGALITAAVEIGALFGKPDLSPEKHVLISEFAARIGLAFQV
                     IDDILDVTATTEKLGKPAGSDTGAGKSTFPSLIGVESARGMAEELLAEALPMLDRVGI
                     KHSLLKDLAELIVKRAF"
     gene            complement(1831..2475)
                     /locus_tag="AC16UZ_00095"
     CDS             complement(1831..2475)
                     /locus_tag="AC16UZ_00095"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HIR6245104.1"
                     /translation="MKYLVRVLCVFSLVMPINAIASALPVEVVGLFKNHAVVRMQGGE
                     FVMKAGETRGGVTLVSADARQARMAYQGREHLVGLSERVSSQYRAVDKAQVMISSDSL
                     GQYWIRGAINNRFTNFLVDTGASVVAISAVHARQLGIEFDESHQGTVQTAQGTADTHF
                     VTLDEIVVGGITAHNVRAAIIEGEYPLEPLLGMSFLRQVSLQENAGVLTLTKQH"
     gene            complement(2527..3015)
                     /locus_tag="AC16UZ_00100"
     CDS             complement(2527..3015)
                     /locus_tag="AC16UZ_00100"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF016489.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HIR6245105.1"
                     /translation="MSTISWRDARDPAVLISCGFGSGFLPGAPGTWGSLAATALWWPL
                     LGGALWWVQAAGALLVLIAGTLLIGRVHRRHDVGDAPAIVIDEFAGMWLVLIGTGQDP
                     LVVAAAFGLFRLFDIWKPWPVRAVEARVRGGLGVMLDDVIAGAMALFVLQVSIWAIGG
                     GF"
     gene            complement(3015..3920)
                     /gene="thiL"
                     /locus_tag="AC16UZ_00105"
     CDS             complement(3015..3920)
                     /gene="thiL"
                     /locus_tag="AC16UZ_00105"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR6245106.1"
                     /translation="MNEFDLIREILDVLGAATGGGAVRVGPGDDAAVIAAPEGADLVS
                     SIDAVVGDVHFPASAGASLIGYRSLMVSLSDLAAMGAEPGFALVALTLPEMDAEWTRQ
                     LASGMARAAQEASAPVVGGNIARGPLTITVSVHGWLPAGSAITRGGARPGHGVYVTGE
                     LGGAAAALTRGGLGERRVEADLDALDARYFLPAPRLEAGKRLRGLASSAIDVSDGLLQ
                     DLGHLCRASGTGAELDGASIPVMAGAKLEHALFGGDDYELCFTAAAEPGELGVACTRI
                     GTVVAGSEITLDARPVSAAGYQHFR"
     gene            complement(3917..4351)
                     /gene="nusB"
                     /locus_tag="AC16UZ_00110"
     CDS             complement(3917..4351)
                     /gene="nusB"
                     /locus_tag="AC16UZ_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR6245107.1"
                     /translation="MNPKPNPWARRRARRALVQAVYQWQITGADAATVTAEFGKALTR
                     ADGDFFDELLRGVLSSAAELDALFAPLLDRGVNELDQVERAILRLGTYELAHRIDVPY
                     RVVIDEYVELAKLFGAEEGYKYVNGVLDGLSHLLREPERRGG"
     gene            complement(4370..5464)
                     /gene="ribD"
                     /locus_tag="AC16UZ_00115"
     CDS             complement(4370..5464)
                     /gene="ribD"
                     /locus_tag="AC16UZ_00115"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR6245108.1"
                     /translation="MAADSDLVYLQSAVELAARGMYTCTPNPRVGCIIVRDGTVLGRG
                     WHVRAGEGHAEVNAIADAGGFVEGATVYVSLEPCAFHGRTPPCSDTLIEARVGRVVAA
                     MTDPHPEVAGRGFAALQQAGIPVEVIEMPQALELNAGYRCRIAKGRPLVRLKTAISLD
                     GRTGMASGESKWITSEAARADVQAWRAQSCAIVSGSGTIMADDASLTVRDERFRVDGT
                     IRQPLRAIADSRLELPADAAVFAGPGQTILFHGPDAEPHVPDIEHVCLGVATGADRGK
                     VDLGGMLAELGRRGCNEVLVEAGARLLGAFIRAGLWDEMIVYVAPKLLGSDARPLAHL
                     PIETMSGAVQAKMIDCEPVGDDLRIRLARA"
     gene            complement(5512..5970)
                     /gene="nrdR"
                     /locus_tag="AC16UZ_00120"
     CDS             complement(5512..5970)
                     /gene="nrdR"
                     /locus_tag="AC16UZ_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR6245109.1"
                     /translation="MHCPFCGADDTKVIDSRLVAEGDSVRRRRECQTCGERFTTFETA
                     ELVMPRIVKSDGTREPFNEDKLRYGLQKALEKRPVSIEEIESALNRIMHRLRASGERE
                     LPSRDVGEAVMTELRTLDQVAYVRFASVYRDFQDLSEFQAEIQKLTFDLE"
BASE COUNT         1080 a         2119 c         1994 g          920 t
ORIGIN      
        1 tcgccagggc cgcccgacag gacgacccac aggcccacaa cgactaggcg tggctgcttg
       61 ctacccacga ggaagccgac cttcgcgacg gcgacctggc catcgagtgt gccgagcgcg
      121 ctgtcgcccg cgagcgcata ccaagctatc tggacaccct ggcagccgcg tacgccgaag
      181 ccggccgatt cgaggacgcc gtcagcaccc aggaacaggc catcgcgatg ctgaacgaag
      241 cggaggggga gattcgcgcg gagcttgccg agcacctgga tgcataccag gccggcagac
      301 cctggcggga ataaggggtt tctggtacaa aacgcgcggc tgaaaagtca gcattcgagg
      361 accaacggtt gagcatcgaa caacgccgca ccaagagaat cttcagggtg atccaggaga
      421 tcgtcggggc gaagggccgc gatttccgtc ctggcgatat cgcctcggtg ctgcgcgagc
      481 gcggccagcc gctcggggcg tgggaaattc gcggccagct gtccattttg gaggcagagg
      541 ggctcatccg gctcgtaccg gaaagtggca gctgggagat ggcgtcctcc gagaaccacc
      601 gcgcggtcag ctgatcggat tcgccaccgg cgcccgagca tcgtttcctt gcgcccccaa
      661 cccgtgccgg ttaccgtttg gtgatggcga aaacctccga gacccccgac gacctttccg
      721 acgagctcga tttcgaatcg gcgctcgagg acctcgaatc cctcgtggaa agcatggagt
      781 ccggcgagat gtccctggag gaatcgctgc gcgccttcga acgcggcatc aagctgaccc
      841 ggcactgcca gtccgcgctg aaagcggcgg agctcaaggt acgcatgctc accgagaacg
      901 acgagctcgt tgcgctggac gccgacgacc cgccaccgga atgactcccg aactcgacga
      961 gctacgcgcg ctcatcaacg acgcgctcga tcaccacctt cccagttctt ccgccatcgc
     1021 ccaacccctg gtggacgcca tgcgttacac caccctgggc ggaggcaaac gcatccgccc
     1081 gatgctggtg tgcgcaacct gcaccgggct cggcggggcg ctggaagacg cgctggcgcc
     1141 ggcgtgcgcg gtggagttca tccacgccta ctccctgatc cacgacgacc tgccgtcaat
     1201 ggacgacgac gagctgcgcc acgggctgcc cagcagccac atcaagttcg gcgaggccac
     1261 ggcggtgctc gccggcgacg ccctgcaggc gctggcgttc gagactctgt cgagggcacc
     1321 ctcggggtcc gacgcccagc ggctgcgcgc cgtgcagctg ctcgccagag ccgccggacc
     1381 ccaggggatg gtgggcgggc agagctacga tctggaatcc gagcggaaga agctgagcct
     1441 gagccagctg cagaccctgc acgcggccaa gaccggagcg ctgatcacgg cggcggtgga
     1501 gatcggcgcg cttttcggca agcccgatct ttcccccgag aaacacgtgc tcatctccga
     1561 gttcgccgcc aggatcggtc tcgctttcca ggtcatcgac gacatcctgg acgtcacggc
     1621 caccacggaa aagctcggca aaccagccgg ctccgacacg ggagcaggca agagcacgtt
     1681 tccttcgctg atcggcgtcg agtccgcgcg cgggatggcc gaagagctgc tggccgaagc
     1741 gctgcccatg ctggatcggg tcggtatcaa gcactcgctg ctcaaggatc tcgcggagct
     1801 gatcgtcaag cgggccttct gaaccctcgc tcagtgctgc ttggtgaggg tgagcacccc
     1861 cgcgttttcc tgcaggctca cctggcgcag gaacgacatg cccagcagcg gctcgagcgg
     1921 gtactcgcct tcgatgatgg cggcgcgcac gttgtgggcc gtgatgccac cgaccacaat
     1981 ctcatccaac gtgacgaaat gcgtgtcggc agtgccctgc gcggtctgca ccgtgccctg
     2041 gtgcgactcg tcgaactcga tgcccagctg ccgggcatgc accgcgctga tggcgaccac
     2101 cgacgcgccg gtgtcgacga ggaagttggt gaagcgattg ttgatggcgc cgcgaatcca
     2161 gtactggccc aatgaatcgg agctgatcat cacctgggcc ttgtcgaccg cgcggtattg
     2221 actggaaacg cgttcggaca gcccgacgag gtgctcccgg ccctgatagg ccatgcgcgc
     2281 ctgcctggca tccgcggaga cgagggtcac cccgccgcgg gtctcgcccg ctttcatcac
     2341 gaactcgccg ccctgcatgc gaaccacggc gtggttcttg aacagcccca ccacctccac
     2401 gggcaaagcc gaagcgatcg cgttgatcgg catgacgaga gagaacacgc agagaacccg
     2461 cacgaggtat ttcatggtcg tccgtgaacg cgacttgacg gccttcgagt gtagtcagcc
     2521 tcgacgctaa aacccgccgc caatggccca gattgagacc tgcagcacaa aaagcgccat
     2581 ggcgccggcg atgacgtcat cgagcatgac gcccaagcct ccgcgcacgc gggcttccac
     2641 ggcacgaacc ggccagggct tccagatgtc gaacaggcga aacaggccga aggcggcggc
     2701 gaccacgaga ggatcctggc cggtaccgat cagcaccaac cacatacccg caaactcgtc
     2761 gatgacgatg gcgggcgcat caccgacgtc gtggcgtcgg tgcacccggc cgatgagcag
     2821 cgtgcccgcg attagcacca gcaaggcccc ggcggcctgg acccaccaga gcgcaccgcc
     2881 aaggagcggc caccagagcg ctgtcgccgc caggctgccc caagtacccg gcgctcccgg
     2941 cagaaagccg ctgccgaagc cgcaggagat caatactgcg gggtcgcgcg cgtcgcgcca
     3001 ggagatagtg ctcatcagcg aaagtgctgg tagccggcgg ccgaaaccgg ccgagcgtcc
     3061 agcgtgattt ccgaccccgc caccacggtg ccgatcctgg tgcaggcgac accgagctcc
     3121 ccgggctccg cggcggcggt gaagcacagc tcgtaatcgt cgccgccaaa gagcgcgtgc
     3181 tcgagcttcg cgccggccat gaccgggatg gatgcgccgt cgagctcggc cccggtgccg
     3241 ctggcacggc acaggtgccc gaggtcctgc agcagcccgt cggacacgtc gatggcgctg
     3301 ctggccaaac cgcgcagccg ctttcccgcc tcgaggcgcg gtgcgggcag aaagtagcga
     3361 gcgtccagag cgtcgaggtc ggcttccacc cggcgctcac cgagcccacc ccgggtaagg
     3421 gcagctgcag ccccaccaag ctctccggtg acgtacacgc cgtgcccggg acgggcgccg
     3481 ccacgggtaa tggccgaacc cgcgggtaac cagccgtgca cgctcaccgt gatggtcagc
     3541 ggtccacgcg cgatgttgcc gccaaccacg ggcgcggacg cctcttgcgc cgcccgggcc
     3601 atgccggacg cgagctgccg cgtccactcg gcgtccatct ccggaagcgt cagcgctacc
     3661 agggcgaacc ccggctccgc acccatggcg gccaggtccg aaaggctcac catcagcgac
     3721 cggtaaccga tgagggaagc gccagccgaa gcaggaaagt ggacgtcgcc gaccacggcg
     3781 tcgatggagg agacgagatc ggcgccttca ggcgccgcga tcaccgcggc gtcgtcgccg
     3841 gggccaacgc ggacggcgcc acctccggtg gccgcgccca gcacgtcgag gatctctctg
     3901 atgagatcga actcgttcac ccgccccgcc gctccggttc cctcaggagg tgggacagtc
     3961 cgtccagaac cccgttgacg tacttgtagc cctcctcggc gccaaagagc ttggcaagct
     4021 cgacgtactc gtcgatgacc acgcggtagg gaacgtcgat gcggtgggcg agctcgtaag
     4081 tgccgaggcg caggattgcg cgctcgacct gatcgagctc gttcacgccc cggtccagca
     4141 ggggcgcgaa aagcgcatcc agctcggctg ccgagctcag cacgccgcgc agcagctcgt
     4201 cgaagaagtc gccgtcggcc ctagtgagcg ctttgccgaa ctccgccgtg accgtcgccg
     4261 catcggcgcc cgtgatctgc cactggtaca cggcttggac cagggccctt cgggcgcggc
     4321 ggcgggccca cggattcggc ttggggttca ttgctgatag gggtcccgtt caggcgcgcg
     4381 ccaggcggat cctcagatcg tcgcccaccg gctcgcaatc gatcatttta gcctgaaccg
     4441 ccccgctcat ggtctcgatg ggaaggtgag ccagcggccg cgcatcgctg ccgagcagct
     4501 tgggcgcgac gtagacgatc atctcgtccc acaagccggc gcggatgaac gcgcccagca
     4561 gcctcgcgcc agcttccacc agaacctcgt tgcagccccg gcggccgagc tccgccagca
     4621 tcccgccgag gtccaccttg ccccgatcgg ctcccgtcgc aaccccgagg cagacgtgct
     4681 cgatatcggg cacgtggggt tcggcgtccg ggccgtgaaa gaggatggtc tggccgggcc
     4741 cggcaaagac ggcggcgtcc gcgggcagct ccagccggga atcggcgatg gcccgcagcg
     4801 gctgacggat cgtcccgtcc acccggaacc gctcgtcgcg gacggtgagg ctcgcatcgt
     4861 ctgccatgat ggttcccgag ccgctgacga tggcgcagct ttgggcgcgc catgcctgca
     4921 cgtcggcacg cgccgcctcg ctggtgatcc acttgctctc ccccgacgcc attcccgtgc
     4981 gcccgtccag ggatatggcg gtcttgagcc tgaccagggg gcggcccttg gcgatccggc
     5041 agcgatagcc cgcgttcagc tccagggcct gcggcatctc gatgacttca accggtatgc
     5101 cagcctgctg cagcgcggcg aagccccgcc cggcgacctc gggatgcgga tccgtcatgg
     5161 ccgccaccac gcgaccgacc cgcgcctcga tgagagtgtc gctgcacggc ggcgtgcggc
     5221 cgtgaaaagc gcagggctcg agggaaacgt atacggtggc accttccacg aagcccccgg
     5281 cgtcggcgat ggcgttcacc tccgcgtggc cttcaccggc tcgcacgtgc cagccgcggc
     5341 cgagcacggt gccgtcacgg acgatgatgc atcccacacg tgggttcggc gtgcacgtgt
     5401 acatgcctcg cgcggccagc tccacggcgc tttgcaggta gacgagatcc gagtcggcgg
     5461 ccatggcgcg caaatatagg cgttcgcgtc cgccgtggct attcgcccgg gctattccag
     5521 gtcgaaggtg agcttctgta tctccgcctg aaactcgctc aagtcctgaa aatcacggta
     5581 aaccgaagca aaacgcacgt aggccacctg atcgagggtg cgcagctcgg tcatgacagc
     5641 ctcgcccacg tcccgggacg gaagctcacg ctccccgctg gcccgcagcc ggtgcatgat
     5701 gcggttcaag gcgctctcga tctcctcgat gctgaccgga cgtttctcca gcgctttctg
     5761 cagaccgtaa cgcagcttgt cctcgttgaa cggctcgcgg gtgccgtcgc tcttgacgat
     5821 gcgcggcatg acgagctcgg cggtctcgaa ggttgtgaat cgctcgccac aggtctggca
     5881 ttcgcgccgg cggcgaaccg agtccccctc agccaccagc cgggagtcga tcaccttggt
     5941 gtcgtcggca ccacagaagg ggcaatgcac ggcgtctgcg caggcgaagc cggggccttg
     6001 cggctcaggc cgcgctggcc tcgtaaacgg ggaaccggcg gcacagatcc agcaccttct
     6061 gtcggacgct gccgatcatg ttgtcgttgt cgatgtcgtc gaggacgtcg cac
//
LOCUS       DBNHOX010000004         3963 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1478904, whole genome shotgun sequence.
ACCESSION   DBNHOX010000004 DBNHOX010000000
VERSION     DBNHOX010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1478904"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1230)
                     /locus_tag="AC16UZ_00125"
     CDS             complement(46..1230)
                     /locus_tag="AC16UZ_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6245110.1"
                     /translation="MSDLEAFRLETREWLEANCPPGVRNRPGEVVWGGRRCQFPNEDA
                     KIWLERMVEKGWTVPTWPKPYGGAGLNDEEAKVLRQEMARIEANAPLFSFGIAMLAPA
                     LMEFASEEQQLEHLPPIARGDFRWCQGYSEPGAGSDLASLRCRAVRDGDHYVVNGSKI
                     WTSDADVSDWIFCLVRTDPDVPKHEGISFLLIDMETPGVSVTPIELISGASPFCQTFF
                     DDVRVPAANLIGEENKGWTIAKRLLQHERAMLGETATRRGGANRKGLVEVARDALRAP
                     DGPLPDAAVREEIAQFDMDRMCYGATFQRSADAARAGRGPGPETSMFKLYLSELNQRG
                     ADLRQRIVGADGLGWAGEGFDADELQITREWLYGRAATILGGTSEIQMNIIAKRVLGL
                     PD"
     gene            complement(1313..2170)
                     /locus_tag="AC16UZ_00130"
     CDS             complement(1313..2170)
                     /locus_tag="AC16UZ_00130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6245111.1"
                     /translation="MKIGAVFPHLEIGSDPAVLRDWAQTAEDLGYSHLLAYDHVLGAV
                     HAGREPALWGPYTEESAFHEPFVLFGYFAAVTRRVELVTGVIILPQRQTALVAKQAAE
                     IDILSGGRLRLGVGTGWNYVEYDSLGENFANRGKRQEEQIEVLRSLWAEPVIDYSGNY
                     HRIERAGLKPLPGRRIPIWLGGFSDVAYRRAARMGDGFIFGGSIKDGLAGLGKIRGFL
                     AEAGREQAGFGIEAMVNYGAGPDRWRADLEAWEAAGADYVSMRGMDAGLETPQDHIDA
                     LRRYWEAVS"
     gene            complement(2203..2979)
                     /locus_tag="AC16UZ_00135"
     CDS             complement(2203..2979)
                     /locus_tag="AC16UZ_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019408213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6245112.1"
                     /translation="MTPELVKTELRPDGIAVVTIDNPPVNAHSGQVLTEMAWTFDAIS
                     DQDEARVVVLTGAGKVFCAGADIKSRSGRAPEPGDHWQSSRAAREAYHSVRECVKPVI
                     GALNGPALGGGLAFAASCDMLIAAETACLGLPEIDVGLLGGGRHAQRLFGHSRLRRMM
                     LTGYRVYGPELYRLGIVEACVAPDALMDEALALAAEVAAKSPIAIRLAKHALNTIEEL
                     SLRDGYRFEQTMTGQLAKTEDSKEAMRAFVEKRKPQFKGR"
     gene            3111..>3963
                     /locus_tag="AC16UZ_00140"
     CDS             3111..>3963
                     /locus_tag="AC16UZ_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6245113.1"
                     /translation="MSKSQPNQLLLTNARLFPGVSDEVFADGAVWIEGRTIRYAGPGC
                     DLPAVADDVPRVDLHGQFVMPGMTESHSHLSYSNNGPTELDKSPVEDAMIHAVANARL
                     MLGSGFTSAISFGSVHRIDVFLRDAIDAGRIPGPRLEASSRDVGATSSNADLHPDYFK
                     PQMEGLGMLADGPWAVRKAVRTIRKNRGNVVKLFLDGEGPGGHAPPGELTYTDEEVAA
                     AVDEAHRRGMRVACHSRSAAAVKQAVRFGIDFIGHANYLDDEALDMLKRSRERLFVGP
                     AIAWELAF"
BASE COUNT          660 a         1388 c         1257 g          658 t
ORIGIN      
        1 cccgcagcag ttcctgctct tcgctgagta ccagaaccac ggcggctaat ccggcaggcc
       61 aagcacgcgc ttggcgatga tgttcatctg aatctccgag gtcccgccca ggatggtggc
      121 cgcacgcccg tagagccact cacgggtgat ctgcagctcg tccgcatcga acccctcgcc
      181 ggcccagccg agcccatcgg cgccgacgat gcgctgacgc aggtcggccc cgcgctggtt
      241 cagctcggaa agatagagct tgaacatcga ggtctccggc ccgggaccgc ggccggctct
      301 ggccgcgtcg gcgctgcgct gaaacgtcgc gccgtagcac atgcggtcca tgtcgaactg
      361 agcaatctcc tcgcgcacgg cagcgtcggg cagcgggcca tccggcgcac gcagggcgtc
      421 ccgcgccacc tccaccagtc ctttacggtt ggcgccgccc cggcgcgtgg cggtctcgcc
      481 aagcatggcg cgctcgtgct gaagcagccg cttggcgatg gtccagccct tgttctcctc
      541 gccgatgagg ttggccgcgg ggacccgcac gtcgtcgaaa aaggtctgac agaagggcga
      601 ggcgccgctg atcagctcga tgggggtgac ggagacaccg ggtgtttcca tgtcgatcag
      661 gaggaagctg atcccctcgt gcttgggcac gtcgggatcg gtgcgcacca ggcagaaaat
      721 ccagtcggaa acgtccgcgt ccgaggtcca gatcttggag ccgttcacca cgtagtggtc
      781 gccgtcccgc acggccctgc agcgaagcga cgcgaggtcg gaaccggccc ccggctcgct
      841 gtagccctga caccagcgga aatcgccgcg ggcgatgggc ggcaggtgct cgagctgctg
      901 ctcttcagag gcgaactcca tcagcgccgg ggcgagcatg gcgatgccga acgagaacag
      961 cggcgcgttg gcttcgatgc gcgccatctc ctggcgcaga actttcgcct cctcgtcgtt
     1021 caggccggcc ccgccataag gcttgggcca ggtgggcacg gtccagccct tctcgaccat
     1081 gcgctcgagc cagatttttg catcctcgtt cggaaactgg cagcggcgcc cgccccagac
     1141 cacctctccg ggccggttgc ggacacccgg cgggcaattg gcctcgagcc actcacgggt
     1201 ctcgaggcgg aaagcttcca gatcgctcat tgcatccctt ttcccagaca gcaaccgctc
     1261 attctagcct gtccaaaggg ctcggtcatg acgcaatcgc ccctccggca actcacgata
     1321 cggcttccca gtaacgtctg agcgcatcga tgtgatcctg cggcgtctcg agtccggcat
     1381 ccatgccacg catggagacg tagtccgccc cggctgcctc ccaggcctcc aggtccgcgc
     1441 gccagcggtc gggccccgcg ccgtaattga ccatcgcctc gatgccgaag ccggcctgct
     1501 ccctgcccgc ctcggcgagg aagccccgga tcttcccgag gccggcaagc ccatccttga
     1561 tgctgccgcc gaagatgaac ccgtcaccca tccgtgcggc gcggcggtag gcgacgtccg
     1621 agaacccgcc cagccagatg ggtatccggc ggccgggcag cggcttcagg cccgcgcgct
     1681 cgatgcgatg atagttgccc gagtaatcga tcaccggctc cgcccagagg cttcgcagca
     1741 cctcgatctg ctcttcctga cgcttgcccc gattggcgaa gttctccccg aggctgtcgt
     1801 actccacgta gttccagccg gtgcccacgc cgagcctgag ccgcccgccg gagaggatgt
     1861 cgatctccgc cgcctgcttg gccaccagcg ccgtctgccg ctggggcagg atgatgaccc
     1921 cggtgaccag ctcgacccgg cgggtcactg ccgcaaaata tccgaagagc acgaacggct
     1981 catggaaagc cgactcttcc gtgtaaggcc cccacagcgc gggctcacgc ccggcgtgca
     2041 ccgcgcccag cacgtgatcg taggccagaa ggtggctgta gccgagatcc tcggcggtct
     2101 gggcccagtc gcgaaggacc gccggatcgc tgccgatctc cagatgcgga aagaccgccc
     2161 ctatcttcat ttcattctcc ctgtatcaga cgagccgagc cttcagcggc ctttgaactg
     2221 aggtttgcgt ttctccacga atgcccgcat ggcttccttc gaatcctcgg tcttagccag
     2281 ctgtccggtc atggtctgct cgaagcggta accgtcgcga aggctcagct cctcgatggt
     2341 gttgagcgcg tgcttggcaa gccggatggc gatggggctc ttggccgcga cctcggccgc
     2401 gagggcgagc gcctcgtcca tgagcgcatc cggggccacg caggcctcca cgatgccgag
     2461 ccggtaaagc tcgggaccgt atacccgata tccggtgagc atcatccgcc gcagccggga
     2521 gtgaccgaac agccgctgcg cgtggcggcc gccgccgagc aggcccacgt cgatctcggg
     2581 aagaccgaga caggcggtct ccgcggcaat cagcatgtcg caggaagcgg caaaggccag
     2641 gccgccgcca agggccggcc cgttgagcgc cccgatcacg ggcttcacgc attcgcgcac
     2701 gctgtggtaa gcctcccggg ccgcccggct gctctgccag tgatcgccgg gttcaggtgc
     2761 gcgtccggag cgggacttga tgtccgcgcc cgcgcagaag accttccccg cgcccgtgag
     2821 cacaactacc cgtgcttcgt cctggtcgct gatggcatcg aacgtccacg ccatctcggt
     2881 gagcacctga ccgctgtgag cattgaccgg cggattatcg atggtcacca cggcgatgcc
     2941 gtccggccgc aattccgtct tcaccaattc gggtgtcatg aaccctcccc caaggactgc
     3001 tgacggggtc cattgtaacg ctctggatct gagccgctac caaggcccgg catcgtggtt
     3061 taagcttttc ccacggtctg gcggaaccac cgcggcaatc ggaggcagac atgagcaaga
     3121 gccaacccaa ccagctgctg ctgaccaacg cgcgactctt tcccggagtg agcgacgagg
     3181 tcttcgccga cggagccgtc tggatcgagg gccgcaccat ccgctacgcc ggcccgggct
     3241 gcgatctgcc cgccgtggcc gacgacgtgc cccgcgtcga cctccacggg cagttcgtca
     3301 tgcccggcat gacggagtcc cattctcacc tttcctactc caacaacggc cccaccgagc
     3361 tggacaagtc tccggtggag gacgccatga tccacgccgt ggcaaatgcc cgtctgatgc
     3421 tcggctcggg cttcacgtcg gccatcagct tcggatccgt gcaccgaatc gacgtctttc
     3481 tccgcgatgc catcgatgcg ggcagaatac cggggccgcg cctggaggcg tcgagccggg
     3541 acgtgggggc gacgtcgagc aatgccgatc ttcacccgga ctatttcaag ccccagatgg
     3601 agggcctcgg catgctggcc gatgggccct gggccgtgcg caaggccgtg cgaaccatcc
     3661 gcaagaaccg cggcaacgtg gtcaagctgt tcctggacgg cgaggggccc ggcgggcacg
     3721 ccccgcccgg ggagctgacg tataccgacg aggaagtcgc ggcagccgtc gacgaggccc
     3781 accggcgcgg catgcgggtc gcctgccatt cgaggtctgc cgcggcggtg aagcaggccg
     3841 tgcggttcgg gatcgatttc atcgggcacg ccaactacct cgacgacgag gccctggaca
     3901 tgctgaaacg aagcagagag cgcctgttcg ttggtcccgc catcgcctgg gagctggcct
     3961 ttc
//
LOCUS       DBNHOX010000005         2915 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1221792, whole genome shotgun sequence.
ACCESSION   DBNHOX010000005 DBNHOX010000000
VERSION     DBNHOX010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1221792"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="AC16UZ_00145"
     CDS             complement(<1..391)
                     /locus_tag="AC16UZ_00145"
                     /inference="COORDINATES: protein motif:HMM:NF016158.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIR6245114.1"
                     /translation="MKQFRRELDARQSDPAGRRWVLVPYDQLTDAVGPLSREDPAELG
                     IVLVESRWKARRRPYHRQKLALVLSNLRHFALEQAERGVAVHHVVGDATYSRLLEPVV
                     VELGPLRVMRPAERELRSDLEPLVKKKR"
     gene            complement(388..852)
                     /locus_tag="AC16UZ_00150"
     CDS             complement(388..852)
                     /locus_tag="AC16UZ_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245115.1"
                     /translation="MTGEMLSLLVLLLHFAATFYMVGLVWFVQRVHYPLYAGVGSQQF
                     PAYERAHVSRTTPVVGPPMLIESATALALVALPMPNASHLLPWLGLGLLAVIWLSTSL
                     LQVPRHRELSAGFDTSAYRRLVATNWIRTSAWSLRGLLVLWMVSSAWSGASA"
     gene            complement(881..1474)
                     /locus_tag="AC16UZ_00155"
     CDS             complement(881..1474)
                     /locus_tag="AC16UZ_00155"
                     /inference="COORDINATES: protein motif:HMM:NF016705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOUL family heme-binding protein"
                     /protein_id="HIR6245116.1"
                     /translation="MAIEKLAYRTIERDGPFELRLIEPHVVAETFVEGDFERVGNEGF
                     RRLVSYIGGANRSQARISMTAPVVQEPASEEIAMTAPVAHEKVGDRYRITFLMPSKYT
                     LENLPQPTDTRIRLRAEQKRRVAAIRYSGFWSRSRYDDHERKLREWIDERGLEPISEP
                     VWARYDPPFMPWFLRRNEVVIEVREPGTATAREESEQ"
     gene            complement(1620..>2915)
                     /locus_tag="AC16UZ_00160"
     CDS             complement(1620..>2915)
                     /locus_tag="AC16UZ_00160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6245117.1"
                     /translation="DRRRPRNATRDGTGVSALSQTIVIVGAGVSGLCCAIHLQERGLR
                     VLVLEADDEVGGRVRTDCIDGFLLDRGFQVLLTAYPEAKAMLDLDALRLGSFAPGAMV
                     RTNSRFARVADPVRRPGEALRTLFSGLATPLDALRIARLRHRVSRGIPLDLLRKPSQG
                     TLDALKGEGFSERIIEGFFRPFFGGVMLDRELRGSSRALEFLFRMFAEGEAALPAQGM
                     GAIPSQLAARLTPGTLRTRTRVARIDRSGVTLESGERIDARAVVVATAARAAAKLIPG
                     VAVPEANSACSLQFDAPAAPPVGDFLVLNGTDEGPVNELCVPSQVVPSYAPAGRALVS
                     ASVLAPVIRDDEALEAAVRSQLTNWFGPEVSGWRLLRIDRIPDALPSQPPGPFQPECR
                     TTHLSDRLFNCGDHRDLASLQGAMASGRRAAMEVAEAAG"
BASE COUNT          513 a          969 c          979 g          454 t
ORIGIN      
        1 tccgcttctt cttgaccaga ggttcgagat cgctgcgaag ctcgcgctcg gcaggacgca
       61 tcacccgaag cggtcccagc tcaacaacga ccggctcgag aaggcggctg tacgttgcgt
      121 ccccgacgac gtggtggacg gcaacgccgc gctcggcctg ctccagcgca aagtggcgca
      181 ggttggacag caccagcgca agcttctggc ggtggtaggg gcgccttcgt gccttccagc
      241 ggctctcgac caggacgatt cccagctccg ccgggtcctc gcgagagagc gggcccacgg
      301 cgtcggtgag ctggtcgtac ggaacgagga cccaacgccg cccggcggga tcgctctggc
      361 gcgcgtccag ctcgcgacgg aactgcttca cgccgaggct ccggaccagg cgctagagac
      421 catccacagc acgagcaggc cgcgcagcga ccacgcagac gtccggatcc agttcgtggc
      481 gacgaggcgt cgataggcgg aggtgtcgaa tcccgccgac agctcgcggt gacgcggcac
      541 ctggagcagt gaggtcgaga gccagatgac ggccaggagc ccgaggccca gccagggcag
      601 aaggtgcgag gcattgggca tcggcagcgc gaccagcgcc agagccgtcg cggactcgat
      661 cagcatgggc gggcccacga ccggggtggt gcgcgatacg tgtgcgcgct cgtaggcggg
      721 gaactgttgc gacccaacgc cggcgtacag cggatagtgg actcgctgta cgaaccacac
      781 caggccaacc atgtagaacg tggccgcgaa gtggagcaag agcaccagaa gcgaaagcat
      841 ttcaccagtc atgacgcctc gaccaggccg ccaagtgccc tcattgctcc gactcctcgc
      901 gagccgtggc agtcccgggc tcgcgtacct cgatcaccac ctcgttgcgg cgaaggaacc
      961 agggcatgaa gggcgggtcg tagcgcgccc atacgggttc gctgatgggc tcgaggcccc
     1021 gttcgtcgat ccactcgcgc agtttgcgct cgtggtcgtc gtagcgtgag cggctccaga
     1081 agcccgaata tcggatcgcg gcgacgcggc gcttttgctc ggcgcgcaat cggattcgtg
     1141 tatcggtggg ctgcggaagg ttctcgaggg tgtacttcga gggcatgagg aacgtgatgc
     1201 gatagcgatc gcctaccttc tcgtgggcga caggtgcggt catcgcgatc tcctcggacg
     1261 ccggctcctg caccacaggc gccgtcatcg agatcctggc ctgactacgg ttggctccgc
     1321 cgatgtagct gacgagccga cggaaccctt cgttgcccac acgctcgaaa tcgccctcca
     1381 cgaaggtctc cgccaccacg tgcggctcga tcagacgcag ctcgaacgga ccgtctcgct
     1441 cgatcgtacg gtaggcgagc ttttcgatgg ccattgcatc tccagccgtg aggatgccaa
     1501 tcgccaggat cattgtgctg acccgcaaga acccggtcac gggccaccct tccgcaggca
     1561 cgtgtcgaga ttctcgttgg cagattccca ttcttccact ttgcatgtcc ccaatgccgt
     1621 catcccgccg cttctgcgac ttccatggcc gctcggcgtc cggaagccat cgctccctga
     1681 agagatgcga gatcccggtg gtcgccacag ttgaacaggc gatccgaaag gtgcgtggtg
     1741 cggcactcgg gctggaacgg accagggggc tgcgagggca gcgcatcggg aatccgatcg
     1801 attcggagca gtctccagcc cgaaacctcc ggtccgaacc agttggtgag ctggctacgt
     1861 acggccgcct ccagggcctc gtcgtcacgt attacgggcg cgagcacgct ggccgatacc
     1921 agggcgcgac cggccggtgc gtagctcgga accacctgac tcgggacgca gagctcgttg
     1981 accggacctt cgtcggtccc gttgagaacc aggaagtccc cgacgggggg tgcagccggc
     2041 gcgtcgaact ggagagagca ggccgagttg gcctccggga cggcgacgcc gggaatgagc
     2101 ttggccgcgg ctcgggcggc cgtcgcaacg accacggccc gagcgtcgat ccgctcgccc
     2161 gattccagcg tgacgcccga gcggtcgatc cgtgcgaccc gcgtccgtgt ccgcagcgtc
     2221 ccgggcgtga gccgggcggc gagctgagaa ggaattgcgc ccattccctg tgccggaagc
     2281 gccgcctcgc cttcggcgaa catgcggaag aggaattcca aggcgcggct ggatccgcgc
     2341 agctcgcggt cgagcatgac gccgccaaag aacggccgga agaatccttc gatgatgcgc
     2401 tccgagaatc cctcgccctt cagcgcatcg agcgtccctt gggacggctt cctgagcagg
     2461 tccagcggga tcccgcggct cacccggtgt cgcaggcgtg caattcgcag tgcgtccagc
     2521 ggggtcgcca aaccggagaa gagagtgcgg agcgcctctc cagggcggcg gaccgggtcc
     2581 gcgacgcgtg cgaagcggct gttcgttcgc accatggccc cgggcgcgaa gctcccgagg
     2641 cgcaaggcgt ccaggtcgag catggccttg gcttccgggt atgccgtcag gagaacctga
     2701 aagccccggt cgagcaggaa gccatcgatg caatcggtgc gcacgcgacc ccccacctcg
     2761 tcgtccgcct cgaggacgag aacccgcagg cctcgttcct gcaggtggat cgcacagcac
     2821 aggccggaaa cgccggcacc gacgatcacg atcgtctggc ttagcgcgct cacgcccgtg
     2881 ccatcccgag tggcgtttct aggccgccgt cgatc
//
LOCUS       DBNHOX010000006         1564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_579086, whole genome shotgun sequence.
ACCESSION   DBNHOX010000006 DBNHOX010000000
VERSION     DBNHOX010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_579086"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="AC16UZ_00165"
     CDS             <1..1052
                     /locus_tag="AC16UZ_00165"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6245118.1"
                     /translation="LPRVRRPATGAEEGVLRTGARRGTGQLIGGGGLEFREIENGEIV
                     LRVALSGHGPVILCVHGWPELWYSWRHQLRHFAERGYTVAAMDVRGYGGSSKPHAIEA
                     YRMTELCADVAAVARELSDEPVVLFGHDWGAPIVWNTALRHPESVRAVAGLSVPYLPA
                     GDVGTLDLFRGIYAGRFFYQLYFQEEGVVEAEVEGDFETALRKIYFSLSGDAPLDDWL
                     KNKPADASLLDGMVDPRPFPEWMSEEDLGVYVDAFTAGGFRGPINRYRAQNLDVAESG
                     PLRGLPVEQPACFVGGERDAVRRFVPGMDLYADPGAACADFRGSTIIAGAGHWVQQEA
                     PEATNEALQRFLDGL"
     gene            complement(1153..>1564)
                     /locus_tag="AC16UZ_00170"
     CDS             complement(1153..>1564)
                     /locus_tag="AC16UZ_00170"
                     /inference="COORDINATES: protein motif:HMM:NF019896.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HIR6245119.1"
                     /translation="SGFPKYVVLRPQLVQKLELVLGELNAVNPDVTGLTVMSGYRTPA
                     YNRSLGNGRHSRHLWGGAADVYVDANGDGIMDDLNGDGRRDRGDAEWLATFIDRLEHA
                     GRFGEHIGGIGTYNSNAHHGPFVHVDARGYRARW"
BASE COUNT          226 a          490 c          573 g          275 t
ORIGIN      
        1 ggttgccgcg cgtccggcgc ccggccacag gcgccgaaga gggggtgctg cgaacagggg
       61 ctcggcgggg aaccggccag ctgatcgggg gagggggact ggaattccgg gagatcgaaa
      121 acggcgagat cgtgctccgc gtcgcgcttt ccgggcacgg gccggtgata ctttgcgtgc
      181 acggctggcc tgagctctgg tactcgtggc gccatcagct gaggcacttt gccgagcgcg
      241 gatacaccgt agctgccatg gacgtgcgcg gttacggcgg cagctccaaa cctcacgcca
      301 tcgaagccta ccggatgacc gagctttgcg cggacgtggc ggccgtggcg cgagagctca
      361 gcgacgagcc cgtcgtgctg ttcggtcacg actggggcgc ccccatcgtc tggaacacgg
      421 cgctgcggca tccggagtcc gtgcgcgccg tggcgggctt gagcgtgccc tacctgccgg
      481 ccggcgacgt tggcaccctg gatctgttcc gcgggatata cgccggccgg ttcttctacc
      541 agctctactt tcaggaggag ggcgtcgtgg aggccgaggt ggaaggggac ttcgagaccg
      601 cgctgcgcaa gatctacttc tcgctgtctg gtgatgcgcc gctggacgat tggttgaaaa
      661 acaagccggc cgacgcctca ctgctggatg ggatggtcga tccccgtccc ttccccgaat
      721 ggatgagcga ggaagacctg ggggtctacg tggacgcgtt taccgcaggc ggcttccggg
      781 ggcccatcaa ccgctatcgc gcgcagaacc tcgacgttgc cgagtcgggg ccgttaaggg
      841 ggctgcccgt ggagcagcct gcgtgcttcg ttggcggcga gcgcgacgcg gtgcgacgtt
      901 tcgtgcccgg catggatctg tacgcggatc cgggcgctgc gtgcgccgat ttccgcggat
      961 cgacgatcat cgccggtgcc gggcattggg ttcagcagga ggcaccggag gcgacgaacg
     1021 aggctctgca gcgtttcctg gatggtctgt agctctgagt cgtgagccaa gcggccgtca
     1081 attcggacgc ttgatgcggg cccacgttcg ggcggcggcg ctcaggccag gcaggccgtc
     1141 gcgttcgccg cttcaccacc gagcccggta accgcgcgcg tcgacgtgga cgaaggggcc
     1201 gtggtgggcg ttgctgttgt aggtgccgat gccgccgatg tgctcgccga agcgccctgc
     1261 gtgctccagt cggtcgatga aggtcgccag ccactcggcg tcgccgcggt cgcgccggcc
     1321 atcgccgttg agatcgtcca tgatgccgtc gccgttggcg tccacgtaga cgtcggcggc
     1381 gccgccccaa aggtgccggc tgtgccggcc gttgcccagg gatctgttgt aggccggggt
     1441 gcggtagccg ctcatcaccg tcaggcccgt gacgtccggg ttgacggcgt tcagctcacc
     1501 gagcacgagc tccagcttct gcaccagctg tgggcgcagg accacgtact tggggaatcc
     1561 cgaa
//
LOCUS       DBNHOX010000007         2201 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_707754, whole genome shotgun sequence.
ACCESSION   DBNHOX010000007 DBNHOX010000000
VERSION     DBNHOX010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_707754"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..90)
                     /locus_tag="AC16UZ_00175"
     CDS             complement(<1..90)
                     /locus_tag="AC16UZ_00175"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR6245120.1"
                     /translation="MVSGIGNVGFVGLGVMGYPMAGHLARAGYT"
     gene            complement(124..1194)
                     /locus_tag="AC16UZ_00180"
     CDS             complement(124..1194)
                     /locus_tag="AC16UZ_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR6245121.1"
                     /translation="MLKFHDLEVAGCTSAGEEALCLQIVVPPELREAFSGAAGQHVVL
                     KADIDGEEVRRSYSLVSSPGEFPLRIGVRLHADGRMSSYLSRLTPGARLSVMPPLGSF
                     ASQCQGPAKRRYAAFAAGCGITPVLALVRAKLAEEPGCRWTVFYGNRSAGRAMFLDEL
                     LGLKDRYLGRLALHFVMSREPSEAEMFNGRIDAAKVEASAGKLFDPAATDEFFLCGPG
                     DMIAAVGAALGKLGVAPDRIHSEHFTVESVAAADRDGEPPPRTGGAAEIIVIMDGRRR
                     SFTMSPDDESILDAALDSGIDLPYSCCAGVCSTCRTRVVEGAAEMAENFSLEDWEIEN
                     GYVLACQSRPTSSELVLDYDDV"
     gene            complement(1213..1704)
                     /gene="paaD"
                     /locus_tag="AC16UZ_00185"
     CDS             complement(1213..1704)
                     /gene="paaD"
                     /locus_tag="AC16UZ_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HIR6245122.1"
                     /translation="MVDEQPVRIIAAEAGEDSVWAVLRGVPDPEIPVLSVVDLGIVRY
                     VRMPGPQVGITPTYSGCPATEVIRASIRSALAQSGYPNARIEEVLSPPWSAAWISAEG
                     RRKLLEYGIAPPNPSSPDEPESCPHCGGRKIEMISQFGSTPCKALFRCLSCREPFDYF
                     KCH"
     gene            complement(1698..>2201)
                     /gene="paaC"
                     /locus_tag="AC16UZ_00190"
     CDS             complement(1698..>2201)
                     /gene="paaC"
                     /locus_tag="AC16UZ_00190"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:TIGR02158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HIR6245123.1"
                     /translation="LVEQPNGDFGCTVVRQFLFDAWQLEMYQGLEASSDARLAEIAAK
                     ALKEVRYHLRYSSGWLVRLGDGTQESHRRVQGPLESLWKYTTELFSPDDLDLEMQAAG
                     VGPDLEVLEAAWSRQVDDVLADATLVRPADVPYGWFGKRGEHTEHLGHLLAEMQVLQR
                     TYPGASW"
BASE COUNT          405 a          765 c          676 g          355 t
ORIGIN      
        1 ggtgtagccc gcgcgggcca ggtgtccggc catgggataa cccatgacgc cgaggccaac
       61 gaaacccaca ttgccgatcc cgctcaccat ccacctcctc tggcttggag ccacactggc
      121 tgcctacacg tcatcgtagt cgagcaccag ctcgctgctg gtcgggcggg actgacaggc
      181 cagcacgtac ccgttctcga tttcccagtc ttccagcgaa aaattctcag ccatctccgc
      241 cgcaccttcc acgacgcggg tacgacacgt ggagcacacg ccagcgcaac aggagtaagg
      301 cagatcgatc ccggaatcca gcgcggcatc caggatggac tcgtcgtccg ggctcatggt
      361 gaacgatcgg cgacggccgt ccatgatcac gatgatctcc gccgcgccac ccgtccgtgg
      421 aggcggctcc ccgtcgcgat ccgccgcagc cacgctttcc accgtgaagt gctcgctgtg
      481 aatccggtcc ggggcaacgc ccagcttgcc cagggcggcg ccgaccgccg cgatcatgtc
      541 gccggggccg cacaggaaaa actcgtccgt cgcggcgggg tcgaagagct tgccggcact
      601 tgcttcgacc ttggccgcgt cgatgcggcc attgaacatc tcggcctcgc tcggctcacg
      661 gctcatcacg aagtgcaggg cgagccggcc gaggtagcga tccttgaggc ccagcaactc
      721 gtcgagaaac atggcccggc ccgcgctgcg gttgccgtag aacaccgtcc acctgcagcc
      781 cggctcttcg gcgagcttgg cgcgcacgag cgcgagcacc ggggtgattc cacacccggc
      841 cgcgaaagcc gcgtagcggc gcttcgccgg tccctggcat tgactggcga agctgccgag
      901 cggcggcatc accgaaaggc gcgctcccgg cgtcagccgg gaaagatacg acgacatccg
      961 tccgtcagca tgcaggcgaa ccccgatacg cagcggaaac tcgcccgggc tgctgaccag
     1021 ggagtaggat cgtctgacct cctcgccgtc gatgtcggct ttcaacacga cgtgttgccc
     1081 ggccgcaccg ctaaaggctt ctcgcagctc cggcggcacg acgatctgca ggcaaagggc
     1141 ttcctcgcct gcagacgtgc acccagccac ttcgaggtcg tgaaatttca gcaactcagg
     1201 aaatctccgc cgtcagtgac acttgaagta gtcgaaaggc tctcgacagg aaaggcacct
     1261 gaagagcgcc ttgcagggag tggagccgaa ctggctgatc atctcgattt tacgaccgcc
     1321 gcaatgcgga caggactccg gctcgtcggg cgaggacggg ttcggcggcg ctatgccgta
     1381 ctccaggagc ttgcggcgac cctcggcgct gatccaggcg gcggaccagg gcggtgagag
     1441 cacctcctcg atccttgcat tcgggtagcc gctctgggca agggcgctgc gaatggatgc
     1501 gcgtatcacc tccgtggccg ggcaccccga gtaggtcggc gtgatcccta cctgcggacc
     1561 cggcatgcga acgtagcgga cgatgcccag gtccacgacg ctgagcaccg ggatctcggg
     1621 atcgggcaca ccacgcaaga ccgcccagac cgagtcttca ccggcttccg ctgcgatgat
     1681 gcgcaccggc tgctcgtcta ccacgatgcc cccgggtaag tccgctgcag gacctgcatc
     1741 tctgccagca ggtggcccag atgctccgtg tgctcgccgc gcttgccgaa ccatccgtag
     1801 ggcacatcgg ccggccgcac cagggtcgcg tcagccagca cgtcatcgac ctgccgcgac
     1861 caggcggctt cgaggacttc cagatccggg ccgacgccgg ccgcttgcat ttccaggtcc
     1921 aagtcatccg gcgagaagag ctcggttgtg tatttccaga gactctccag aggaccctga
     1981 acacgccggt gactctcctg ggtgccgtca cccagccgaa ccagccagcc gctggagtaa
     2041 cgcaggtgat agcggacttc tttcagcgcc ttcgcagcaa tctctgcaag gcgtgcgtcc
     2101 gaggacgcct cgagcccctg atacatttcc agctgccagg catcgaacag aaactggcgg
     2161 accaccgtgc acccgaaatc gccgttcggc tgctccacca g
//
LOCUS       DBNHOX010000008         2158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_707834, whole genome shotgun sequence.
ACCESSION   DBNHOX010000008 DBNHOX010000000
VERSION     DBNHOX010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_707834"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="AC16UZ_00195"
     CDS             <1..189
                     /locus_tag="AC16UZ_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6245124.1"
                     /translation="VMETHLAAEEFFVAGRYTIADIALYAYTHLAHQGGYDLDPFPAV
                     RAWLARVKAQPRHSPIDA"
     gene            196..1062
                     /locus_tag="AC16UZ_00200"
     CDS             196..1062
                     /locus_tag="AC16UZ_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR6245125.1"
                     /translation="MPEILCLLGEAALARNDNHVRLPEAFAQAGWQVTALPHDCLGVH
                     AGRIHLDGHDPDRFDRIWPLGLGPRETFFDRMQMLAALDQRRLVIGADAFTYLHGKYR
                     WLDLMPETHAANDADALARVIASGGDWVIKPPAGSYGQGVQVIREGEDPMPALRSLTG
                     GEARRYCLVQRYVPEIVRGEKRTLVAGGEVCGTYLRTPTKDHRANLSAGGRASAAKLT
                     EEEARLVEGLTAELLSLGAAFAAIDIVAGRLMEVNVANPGGLATLENVYGRDPTPQAV
                     AALMAYWARTGG"
     gene            1280..2146
                     /locus_tag="AC16UZ_00205"
     CDS             1280..2146
                     /locus_tag="AC16UZ_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6245126.1"
                     /translation="MLDGKVAVVTGAGGGIGRSHAKLLAKEGAAVVVNDLGGARDGTG
                     ASQSMADTVVDEIIADGGRAIANYGSVSDKAAAQAMIDAAVSEFGRIDIMIANAGILR
                     DKSFKNMDDEMWDVVLDVHLRGTYLTVKAAYDRMLEQGEGGRIIMTSSTSGLLGNFGQ
                     TNYGAAKAGIAGFMRCLWLEGLKYGINVNVLVPTATSRLTEDILPEDIQDRFPPETVS
                     PAIVWLCTDEAKEVTGRQWLVAGNNVSLLSWQITPIADRDVSEGPWDVADIGQRILAS
                     KESWPPINPLRG"
BASE COUNT          384 a          737 c          711 g          326 t
ORIGIN      
        1 gtgatggaaa cccatctcgc cgcggaggag ttcttcgtcg ccggccgcta caccatcgcg
       61 gacatcgccc tgtatgccta cacccacctc gcccaccagg gtggctacga cctggatccc
      121 tttccggcgg ttagggcctg gcttgcgcgg gtcaaggcac agccgcgcca cagtcccatc
      181 gacgcctgac cgcccgtgcc ggagattctc tgtctgctgg gagaggcggc actcgcccgc
      241 aacgacaacc acgtgcggtt gcccgaagcg ttcgcccagg cgggctggca ggtcaccgcc
      301 ctgccccatg actgcctggg cgttcacgcc ggcaggatcc acctcgatgg gcacgacccc
      361 gaccgcttcg accggatatg gccgctcggg ctcggcccca gggagacgtt cttcgaccgc
      421 atgcagatgc tcgcggcgct ggaccagcga cgtctggtga tcggcgccga tgctttcacc
      481 taccttcacg gcaagtaccg gtggctggac ctcatgcccg agacccatgc tgcaaacgac
      541 gccgacgccc tggcacgagt aatcgccagc ggcggcgact gggtgatcaa gccccccgcc
      601 gggagctacg gtcagggggt gcaggtgatc cgggaaggtg aagaccccat gcccgcgctg
      661 cgctccctca cgggcggcga ggcgcgacgt tactgcctgg tccagcgcta cgtccccgag
      721 atcgtccggg gcgagaagcg caccctggta gcgggcgggg aagtctgcgg cacctacctg
      781 cgcacaccca cgaaagacca tcgtgccaac ctgagcgcgg gcggtcgggc gagcgctgcc
      841 aagttgacgg aggaggaagc caggctcgtc gaggggctga cggccgagct gctgagcctc
      901 ggcgccgcgt tcgcagccat cgacatcgtg gccggccggc tgatggaagt taacgtggcc
      961 aatccgggcg ggttggccac cctcgagaac gtctacggac gcgacccgac gccccaagcc
     1021 gtggccgcgc tcatggccta ctgggcccga accggcggct gagcccggcg cttgcgtttg
     1081 cggcgcttcg ccttagactg caccgttcgt ccgattcggc gggcgaagga ctgggggttt
     1141 gaacgttccg ctcagcctgc ggcaggcaac gccgctcggg cgcgtcaacc gttgcagcac
     1201 gcactgcagc aagcacctcc cctacgcttc ctgccgaccg gactgctgaa tcgcacagaa
     1261 caaggagatc gatatgggaa tgctcgacgg caaagttgcg gtggtgaccg gagcaggcgg
     1321 cggtatcgga cgatcccacg ccaagctgct ggccaaggaa ggggccgccg tggtggtcaa
     1381 cgacctgggc ggcgcccgag acggcacggg agcatcccag tccatggccg acaccgtcgt
     1441 ggacgagatc atcgcagacg gcggccgggc gatcgcgaac tacggctccg tctccgacaa
     1501 ggccgcagcc caggccatga tcgatgccgc cgtcagcgag ttcgggcgca tcgacatcat
     1561 gatcgccaac gccggcattc tccgcgacaa gtctttcaag aacatggacg acgagatgtg
     1621 ggacgtggtc ctcgacgttc atcttcgtgg cacctacctc accgtcaaag ccgcctatga
     1681 ccggatgctc gaacagggag agggcggccg gatcatcatg accagctcca cctcggggct
     1741 gctgggcaac ttcggccaga ccaactacgg cgcggccaaa gccggcattg cgggattcat
     1801 gcgctgcctc tggctcgagg ggctgaagta cggcatcaac gtcaacgtgc ttgtacccac
     1861 cgcaacgtcg cggctcaccg aagacatcct ccccgaggac atccaggacc gattcccgcc
     1921 ggagaccgta tcgccggcca tcgtctggct gtgcacggac gaggccaagg aggtcaccgg
     1981 ccggcagtgg ctggtcgccg gcaacaacgt ctcgctgctc tcctggcaga tcacccccat
     2041 cgccgaccgg gacgtctcgg aagggccgtg ggacgtggcg gacatcgggc agaggatcct
     2101 ggcgagcaag gagagctggc cgccgatcaa tcctctccgg ggctgaggca ggctccat
//
LOCUS       DBNHOX010000009         1713 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_707922, whole genome shotgun sequence.
ACCESSION   DBNHOX010000009 DBNHOX010000000
VERSION     DBNHOX010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_707922"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1697
                     /locus_tag="AC16UZ_00210"
     CDS             60..1697
                     /locus_tag="AC16UZ_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HIR6245127.1"
                     /translation="MAEEPNDFIRQRIRADLASGVVKGGVVTRFPPEPNGHLHIGHAK
                     SICLNFGVAEEFGGYTYLRFDDTNPLKESEEFALAIQEDVRWLGYDWGDHLTHASDYF
                     EELYGFAVQLIEQGKAYVCSLTGDEIRATRGSLTEPGTESPYRDRSVEENLNLFRRMR
                     EGEFADGEHVLRAKIDMASPNINMRDPVIYRIRHASHQRTGDAWVIYPMYDFTHCICD
                     ALEGITHSLCTLEFEDHRPLYDWVLDNININFHPPQIEFSRLGLEYTVMSKRLLHRLV
                     AEGLVEGWDDPRMPTIAGLRRRGVTPGAIREFCRRIGVTKQDGMVEMNLLEFCIRQDL
                     ESSAPRGMAVLDPLKVVITNHPGAEEYLVAPWHPNVAELGERELPFGPEIYIERADFL
                     EEPPRKYKRLSPTEMVRLRYAYVIRCDEVVRDEVGNIAELRCTYFSDSKSGADTSGLK
                     PKGVIHWVSAATGMPTRIRLYDRLFTVPAPRGEELVGELNTQSLAEMSGFVEPAVAAS
                     AARRFQFERQGYFCKDSQLDGVFNRTVTLRDRWKPAP"
BASE COUNT          317 a          536 c          554 g          306 t
ORIGIN      
        1 agacgccggt ttcggccttt ggttagaata cgcgccccgc agattccgta gatgggccga
       61 tggccgaaga acccaacgac ttcatacgcc agcgcattcg cgcggacctt gccagcggcg
      121 tcgtgaaggg cggcgtggtg actcgatttc cgccggagcc gaacgggcat ctgcacatcg
      181 gccacgccaa gtccatctgc ctgaacttcg gggttgccga ggagttcggg ggctacacct
      241 atcttcgctt cgacgacacc aacccgctca aggagagcga ggagttcgcc ctggccattc
      301 aggaggacgt ccgctggctg ggttacgact ggggcgacca cctcacccac gcctcggact
      361 atttcgagga gctctacggc tttgccgtgc agctgatcga gcagggcaag gcctacgtct
      421 gcagcctgac cggtgacgag atccgggcga cccgggggtc cctcaccgag cccggcaccg
      481 agagtcccta tcgcgaccga tccgtggagg agaacctcaa tctcttccgg cgcatgcgcg
      541 aaggcgagtt tgcggacggt gagcacgttc tgcgggcgaa gatcgacatg gcgtccccga
      601 acatcaacat gcgcgatccc gtgatctacc ggatccgcca tgcttctcat cagcgaacgg
      661 gtgatgcctg ggtgatctac ccgatgtacg acttcacgca ctgcatctgc gatgccctgg
      721 aagggatcac gcactcgctt tgcacgctgg aattcgagga ccaccggccc ctctacgact
      781 gggtgctgga caacatcaac atcaactttc acccgccgca gatcgagttt tcccgcctgg
      841 ggctcgagta cacggtcatg agcaagcgtc tcctgcaccg cctggtggcg gaagggctgg
      901 tggaaggctg ggacgacccg cgcatgccga ccatcgcagg tcttaggcgt cgtggggtga
      961 cccctggagc aattcgcgag ttctgccgcc gtatcggggt gaccaagcag gacggtatgg
     1021 tggagatgaa cctgctggag ttctgcatcc gacaggatct ggaaagctcc gcgccccgag
     1081 gcatggccgt tttggacccg ctcaaggtgg tgatcaccaa ccacccgggc gccgaggagt
     1141 atctggtggc gccgtggcat cccaacgtgg cagagttggg cgagcgcgag ctgccgttcg
     1201 gcccggagat atacatcgag cgcgccgact tcctggagga accgccgagg aagtacaagc
     1261 gtctgtcccc caccgagatg gtgcgcctgc gctacgccta cgtgatccgg tgcgacgagg
     1321 tggtcaggga cgaggtggga aacatcgccg agctgcgctg cacctatttc tccgactcga
     1381 agagcggcgc ggacaccagc ggcctcaagc ccaaaggcgt cattcactgg gtatcggcgg
     1441 ccacgggcat gccaacacga atcaggctgt acgatcggct cttcaccgtc cccgcgccgc
     1501 gtggcgagga gctcgtcggc gagctcaata cgcagtcgct ggccgagatg tccggtttcg
     1561 ttgaaccggc ggtggcggcg agcgccgcga gaaggtttca gttcgagcga cagggctatt
     1621 tctgcaagga ctctcagctc gatggggttt tcaaccgcac ggtgacgctc cgggaccgct
     1681 ggaagcccgc tccttaggcc gtcgcggtgg tcc
//
LOCUS       DBNHOX010000010         9506 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_772383, whole genome shotgun sequence.
ACCESSION   DBNHOX010000010 DBNHOX010000000
VERSION     DBNHOX010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9506)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9506
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_772383"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="AC16UZ_00215"
     CDS             <1..215
                     /locus_tag="AC16UZ_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR6245128.1"
                     /translation="GLWLSLGLFAWAAGADPVWCFAGFVAMVALFELVSIPMIEKKLL
                     AAKPGYEAYRRSTFRLLPFSALKRFS"
     gene            226..957
                     /locus_tag="AC16UZ_00220"
     CDS             226..957
                     /locus_tag="AC16UZ_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6245129.1"
                     /translation="MANFMLIHGAWHGGWCWHKLVAELSRRGHRALAPDLPAHGHDAT
                     PRDRVTLDAYADSVIEHLNDFAGPVCLVGHSMGGMVISAVGQRVPERLERLVYLCAFV
                     PADGMSLLSSDPTESGAQAFAGKIRGGEDGASVMIDDAAVAALFYHDCTDEDVAFARA
                     RLVPQPSAPLGAAVALTDAAFGSLAKSYIECTADQAAPIAAQRAMYTRAGIDSVVSLS
                     TSHSPFFSAPTLLADSLEALIGTPA"
     gene            1058..2752
                     /locus_tag="AC16UZ_00225"
     CDS             1058..2752
                     /locus_tag="AC16UZ_00225"
                     /inference="COORDINATES: protein motif:HMM:NF021354.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPL-N domain-containing protein"
                     /protein_id="HIR6245130.1"
                     /translation="MQRVMISSRRGGYAAFFATIVVALTLGASPADGARGGKKGGGGP
                     VCKITDQTDVAIYVGGGIGPSSQLWAEALIDFWKTGSRIPGEPGLLNDDGGATWTGDG
                     SLDYVTLTLSEFNACRDDDFAKLELFFMPGGSAYEIQANLGASGKDRLTRFLDSGGSY
                     VGMCAGGYYAARGYYWKGDDGAPTDNCRNQFCRYEVDGTFSYEAATSDFTVHEWGGVS
                     YHADLLAYGPLAEVLVEGPIEEIAGPWHAQSDPNHPYDSHLVTTDDPAMPWLRAVYWG
                     GATENYVYTDNAPWGTEHAHFAADASGNDDLPFPTDGSLWTLKSVATGTGGAIMISSA
                     HLEASLFHTASTFTDGGMTECQQYNNYTYLIRRMQSQLGAPFAVPDYDAFCAPDRAGE
                     VKETAVLFPSGLAYQNAPRIDTSGGGGGDGGGDGGGGTGSTSISFDDGTLGDFQLEGD
                     GLRPWQANATAACNGAYGAMAGVPGGVDMESFLTLTVPAGVSRVTYRYSYPSALDRGD
                     DFHVIVNGAVRKSYETGSGAQCATDSLPVTPGDELRFKCRSGGNGETCAVDEIVFD"
     gene            complement(2777..4354)
                     /locus_tag="AC16UZ_00230"
     CDS             complement(2777..4354)
                     /locus_tag="AC16UZ_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR6245131.1"
                     /translation="MTTQAGGAGVPARGGLGAVACGHPATACAAAEILEEGGNAFDAV
                     IAAQFAACVAEPVLASVGGGGFLLAHPAPGAPVVYDFFVQTPRSPREASAVDFRPVHA
                     DFGTATQEFHIGLGAAATPGSLAGMEAVHGDLGSLPMARLVEPAARAAREGVRIERLQ
                     ASIFEIVKPIYAASSEARSIYGGIQEGRLLRQSDLADTLEQVARQGAGRFYRGAFADA
                     VARLAQEQGGHLSMEDLLGYGVVKRMPLSLRHAGATVLTNPPPSSGGILIGFGLQLLQ
                     AMAVADQTFGGPSHLAALAEIMAATNQARVELLAAAGSHAELDPALVERYRREVLGTP
                     RFNRGTTHISVVDAKGNAAAMTLSNGEGCGTMIPGTGVMLNNMLGEEDINPDGFGNWP
                     PDQRLTSMMAPTLVETVAGRRLALGSGGSNRIRTAIVQVISNLLDFGDSVDAAVRRPR
                     LHLEGDLLSIEDGFDRDAVRALEAGPAEIRRWPGQSLFFGGVHVAELAKDLFRAAGDP
                     RRGGVGRVVTARGAPEG"
     gene            complement(4367..5419)
                     /locus_tag="AC16UZ_00235"
     CDS             complement(4367..5419)
                     /locus_tag="AC16UZ_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6245132.1"
                     /translation="MALALALEEHTATREGHTTFYLAAGPEDGPLIIFVHGWPELSLS
                     WRHQLPALAALGYRAVAPDMRGYGRSSTYERYEDYAQRAVVGDMMALLEGLGRTEAIW
                     VGHDWGAPTVWSIASHHPEACTAVAGLCVPYFTLERGLQACVDQVNRDVYGPEYPAGQ
                     WEYQLFYQEHFDRARAEMDANPLNTVKLLFRRGDPNGAGRPAATAMTRINGGWFGPAG
                     EAPDVPRDDDVISEEDLAVYADALARNGFFGPNAYYMNHAENSAYAAEAVNDGYLDMP
                     VLFLGARYDYVCECYDSTLADPMRERCRRLTETTVYSGHWMAQERPLDVNAALVRWLA
                     TDLPEVWPRSRAPDPA"
     gene            5550..6152
                     /locus_tag="AC16UZ_00240"
     CDS             5550..6152
                     /locus_tag="AC16UZ_00240"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR6245133.1"
                     /translation="MRIWHWVLAACVTGSLYTGLAGEIALMERHQQFGYGVLGLLLFR
                     LGWGLWGGRYARFGNYRLAPRRLIEHFRGLDHGAAHTAPGMAFTIVFLLALAAQVGTG
                     LFATDDIFTQGPLSRLVENDTARALTGIHHRVYWLILAALAAHLTAHAVYALRRDPMP
                     LAMITGRKRLDLEPARAAWTAALLTALGAAALVWAMLELA"
     gene            complement(6156..6584)
                     /locus_tag="AC16UZ_00245"
     CDS             complement(6156..6584)
                     /locus_tag="AC16UZ_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013487.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6245134.1"
                     /translation="MRGRDWLIGLVALGAASAATASEGELEYREHTMEAIGGHMQAAV
                     DILRQKVPHASHMTIHADALAALSGIVGTLFPEGSEGGDALPAIWEEPEDFAGRVEAM
                     REAATAFSAAARSNDDAALGQAFQALGQSCKGCHDNYRAD"
     gene            6775..7158
                     /locus_tag="AC16UZ_00250"
     CDS             6775..7158
                     /locus_tag="AC16UZ_00250"
                     /inference="COORDINATES: protein motif:HMM:NF015180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HIR6245135.1"
                     /translation="MQPVPNGMDRFAITLSGLCLLHCLLIPVVLAMAPAFSSLVLGTE
                     TTVHWVFLGMAVPTSVWALWRGYGRCQHLRALVLGIAGLLVMFLGVSHLFDPRLEVPL
                     TLVGVTMVVVAHVLNLRIPEPAPRG"
     gene            7222..7671
                     /locus_tag="AC16UZ_00255"
                     /pseudo
     CDS             7222..7671
                     /locus_tag="AC16UZ_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
     gene            complement(7745..8776)
                     /locus_tag="AC16UZ_00260"
     CDS             complement(7745..8776)
                     /locus_tag="AC16UZ_00260"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR6245136.1"
                     /translation="MSADYRLVEVAEGLELPWCVAFLPDGRLLVTERPGRLRQISPEG
                     EIGSPMEGIPEVLFRAQGGLFDVAPHPDFAENRLIYISYAHGTPRANTTRIARGRLDG
                     NSLKDLEVIFTARPNKTTPQHYGGKFLFLPDGTLLFTTGDGFDHREEAQDLSGLLGKT
                     VRIRDDGSIPDGNPYVRREGADPAVWTWGHRNPQGLAVDHVTGRVFLHEHGPRGGDEI
                     NLLRPGRNYGWPVITFGLDYSGARISPYQQMPGMEQPLHSWVPSIAPSGLAVYRGDVF
                     PEWQGDLFVGALVDREARRVRLRDDRVVEEEALFGELGERLRDIRVGPEGHLYLVTDE
                     GRVLRVERP"
     gene            8915..9355
                     /locus_tag="AC16UZ_00265"
     CDS             8915..9355
                     /locus_tag="AC16UZ_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245137.1"
                     /translation="MAATVIARIAGAYLLSVLVAYLLAATAATQASLAGLEGLGVTIT
                     VGERISTTGQDLMGMLPTYGLLIAIGFAIALPIAAAFARFIPSWRAVGLVAAGAAAVG
                     LIHVGLNSVFETHLVAATRTTVGLLVQVMAGGVGGYAFYRLRDP"
BASE COUNT         1487 a         3121 c         3190 g         1708 t
ORIGIN      
        1 tcggcctgtg gctctcgctc gggctgttcg cctgggcagc tggggcggat ccggtctggt
       61 gcttcgccgg cttcgtcgcc atggtggcgc tcttcgagct cgtgagcatc ccgatgatcg
      121 agaagaagct gctggccgcc aaacccggtt acgaggcgta tcggcggtcc acgttccgcc
      181 tgctgccttt ttccgcgctc aaacgtttct cctagagggc cgatcatggc gaacttcatg
      241 ctgattcacg gtgcctggca cggaggctgg tgctggcaca agttggtcgc cgagctcagc
      301 cgccgggggc accgggccct cgcgcccgat ctgccggctc atgggcacga tgccacgccc
      361 cgcgaccggg tgacgctgga cgcttatgcc gacagcgtta tcgagcatct caatgacttt
      421 gcaggccccg tctgcctggt cggccactcc atggggggca tggtgatctc ggcggtcggt
      481 cagcgggttc ccgagcggct cgagcggctc gtctacctgt gcgcgttcgt gcctgccgac
      541 gggatgtctc tgctgtcctc ggaccccacg gagagcggag cccaggcttt tgccggaaag
      601 atccgcgggg gtgaagacgg tgcctcggtg atgatcgatg acgccgccgt ggccgcattg
      661 ttctatcacg attgcaccga cgaggacgtg gctttcgcga gagcccgcct ggttcctcag
      721 cccagcgctc cgctgggtgc ggccgtcgcc ctgactgacg cggctttcgg cagcctggcc
      781 aagagctaca tcgagtgcac ggcggaccag gccgcgccta tcgctgccca gagggccatg
      841 tatacccgcg ccgggatcga ctcggtggtc agcctaagca cgtcccactc gcccttcttc
      901 tcggccccca ccctgctcgc tgactccctc gaggcgctca tcgggacgcc ggcatgaggc
      961 ctcgggagtg caattggctt acgaatcggt aacaagtcgt cgttgcgtcg agcatgcacg
     1021 ggtcacagac tggcgtgaag gacgggaagg aatacatatg caacgggtca tgatcagctc
     1081 gcggcgtggt gggtatgccg cgtttttcgc aacgatcgtc gtcgccctca cgttgggcgc
     1141 ctcgcctgcc gatggggcga ggggcggcaa gaagggcggc ggtggaccgg tctgcaagat
     1201 caccgaccag acggacgttg cgatttacgt tggcggcggc atagggccca gcagccagct
     1261 gtgggccgag gcgctgatcg acttctggaa aaccgggagc cgcatccccg gtgagcctgg
     1321 gcttctcaac gacgacggcg gggccacctg gacgggcgat ggcagcctgg actacgtcac
     1381 cctcacgctg tcggaattca acgcctgcag ggacgacgac ttcgccaagc tggagctttt
     1441 cttcatgccg ggcgggagcg cctacgagat acaggcgaac ctgggcgctt ccggcaagga
     1501 tcggctgacg cgctttctgg acagcggcgg cagctatgtc ggcatgtgtg ccggcggcta
     1561 ctacgcggcg cgcgggtact actggaaagg tgacgacggc gctcccacgg acaattgcag
     1621 gaaccagttc tgccgctacg aggtggatgg caccttttcc tacgaggcgg caacatcaga
     1681 cttcaccgtg cacgagtggg gcggggtttc ctatcacgcg gatctgctcg cctacgggcc
     1741 gctggccgag gtgctggtgg aggggcccat cgaggagatt gccggtccat ggcatgccca
     1801 gagcgacccc aaccatccct acgattccca cctggtgacc accgacgacc cggccatgcc
     1861 ctggcttcgt gccgtttact ggggaggcgc gacggagaac tatgtctaca cggacaacgc
     1921 accgtggggc accgagcacg cccactttgc cgccgatgcg agcggcaatg acgatctgcc
     1981 gtttcccacg gacgggtcgc tgtggacgct caagagcgtg gcgaccggca ccggcggggc
     2041 gatcatgatc tccagcgcgc acctggaggc ctccctgttt cacacggcca gcacctttac
     2101 cgacgggggc atgaccgagt gtcagcagta caacaactac acgtatctca tccggcgaat
     2161 gcagtcccag ctgggcgcgc catttgccgt gcccgactat gacgccttct gcgctccgga
     2221 cagggccggc gaggtgaagg agacggccgt cctgtttccg tccggcctgg cttaccagaa
     2281 tgcgccgcga atcgacacct ctggtggagg cggcggtgat gggggtggtg atggcggcgg
     2341 tggcaccgga tcgacgtcga tctccttcga cgacggcacc ttgggtgact ttcaactgga
     2401 aggcgacggg ttgcggccgt ggcaggccaa tgcgactgct gcctgtaatg gcgcgtacgg
     2461 cgcgatggcc ggagtgcccg gcggggtgga catggagtcg ttcctgaccc tgaccgttcc
     2521 tgcaggcgtg agccgggtaa cctaccgata cagctacccg agcgctctgg accggggcga
     2581 cgacttccac gtcatcgtca acggcgcggt cagaaagtcc tacgaaacgg gcagcggcgc
     2641 gcagtgcgcg acggattcgt tgccggtgac gccgggggat gagctgcggt tcaaatgccg
     2701 ttccggcggc aacggcgaga cttgcgccgt ggacgagatc gtcttcgact gaaacctcgg
     2761 cgcccggcga gcaaggtcaa ccctctggcg ctccgcgcgc ggtcacaacc cgtccgacgc
     2821 cgccgcggcg gggatcgccg gctgcccgga agagatcctt ggccagctcg gccacatgca
     2881 cgccgccgaa gaacaggctc tggccgggcc accggcgaat ttcggccggg cctgcctcca
     2941 gcgcccgaac cgcatcgcgg tcgaacccgt cctctatgga aagcagatcg ccctcgagat
     3001 gcagccgtgg tcggcgaacc gcggcgtcga cggagtcccc gaagtcgagc aggttgctga
     3061 tgacctgcac gatggcggtg cgaattctgt tcgagcctcc cgatcccagg gccaggcgcc
     3121 tcccggcgac ggtttccacc agggtgggcg ccatcatcga cgtgagccgt tggtctggcg
     3181 gccagttgcc gaacccgtcc gggttgatgt cctcctcgcc cagcatgttg ttcagcatca
     3241 cgccggttcc tggaatcatg gtcccgcacc cctcaccgtt ggaaagggtc atggcggcgg
     3301 cgttgccctt tgcgtccacg acgctgatgt gcgtggtgcc gcggttgaag cgcggcgtcc
     3361 cgagcacctc ccgccggtaa cgctcgacga gcgccgggtc cagctccgcg tggctgccgg
     3421 ccgcggcgag caattccact cgggcctgat tggttgccgc catgatttcg gccagggcgg
     3481 caagatgcga cgggcccccg aaggtttggt cggcaacggc catggcctgg agaagctgca
     3541 ggccaaagcc gatcaggatt ccgcctgagg agggcggagg gttggtcagc acggtggccc
     3601 cggcgtggcg caggctcaac ggcatgcgtt tgacgacccc gtagccgagc agatcctcca
     3661 tggaaaggtg cccgccttgc tcctgcgcca gccgtgccac cgcgtctgcg aacgcgccgc
     3721 ggtagaagcg ccccgcgcct tgccgcgcga cctgctcgag ggtgtcggcc aggtcgctct
     3781 ggcgcaggag gcgcccctct tggatgccgc cgtagatcga ccgggcctcg ctgctcgctg
     3841 cgtagatggg cttgacgatc tcgaagatgc ttgcctgcag gcgctctatg cgcacgcctt
     3901 cccgtgcggc acgagcggcc ggttcgacca gccgagccat gggcaggctc cccaggtcgc
     3961 cgtgaacggc ttccatcccg gccagggagc cgggcgttgc cgcggctccc aggccgatgt
     4021 ggaattcctg ggttgcggtg ccgaagtcgg cgtggaccgg acgaaagtcc acggccgaag
     4081 cctctctagg cgagcgcggc gtctggacga agaagtcgta gaccaccggc gcgcctggag
     4141 cggggtgtgc cagcaggaag cccccgccgc caacggatgc cagcaccggt tcggcgacgc
     4201 aggcggcgaa ctgggcggcg atcaccgcgt cgaaggcgtt gcctccttcc tcgaggatct
     4261 ccgcggccgc gcaggcggtt gccgggtgac cgcaggcgac ggcccccagg ccaccccgcg
     4321 ccgggacgcc cgcccctccg gcttgagtcg tcaaagccag gcagacctag gccgggtcgg
     4381 gggcccgcga gcggggccac acctccggca ggtctgtcgc cagccagcgt accagcgccg
     4441 cgttgacgtc cagcggacgc tcctgggcca tccagtgccc ggagtagacg gtggtctcgg
     4501 taagccgccg gcagcgctcg cgcatgggat cggcaagcgt ggagtcgtag cattcgcaga
     4561 cgtagtcgta gcgggcgccg agaaacagaa ccggcatgtc gaggtagcca tcgttgaccg
     4621 cttctgccgc gtaggcggag ttttcggcgt ggttcatgta gtaggcgttc gggccgaaga
     4681 agccgttgcg ggcgagggcg tcggcgtaga ccgccaggtc ttcttcgctg atcacgtcat
     4741 cgtccctggg cacgtcgggc gcttctccgg caggaccgaa ccagccgccg ttgattcggg
     4801 tcatcgccgt cgccgccggc cggccggcgc cgttggggtc gcccctgcga aacaggagct
     4861 tcacggtgtt cagggggttg gcatccattt cggccctggc cctgtcgaag tgctcctgat
     4921 agaacaactg gtattcccat tgccccgccg ggtactcggg cccgtagacg tcccggttca
     4981 cttggtccac gcacgcctgt aatcccctct ccagggtgaa gtagggcacg cagagcccgg
     5041 ccacggcggt gcaggcctcg ggatgatggc tcgctatgct ccacacagtc ggcgcccccc
     5101 agtcgtgacc tacccagatc gcttccgtgc gtcccaagcc ctccagcagc gccatcatgt
     5161 cgcccaccac cgcgcgctga gcgtagtcct cgtatcgctc gtaggtcgag gatcttccgt
     5221 accctctcat gtcgggggcg acggctcgat aaccgagcgc ggccagcgcg ggtagctgat
     5281 gccgccagct cagggagagc tcgggccagc catgaacgaa tatgatcagc gggccgtcct
     5341 cggggcccgc ggcgagatag aatgtcgtgt ggccctcgcg tgttgcggtg tgctcctcca
     5401 gtgccagtgc cagtgccaaa gcccttctcc cgtgtcttga cctcatcgtg attgttcgaa
     5461 tcatatccct tatggcaaac ggggcaccta ccaggatttc gccgacgggc gcgggcgcct
     5521 ctgcgggcac cctgatctgg gaactgccca tgaggatctg gcactgggta ttggcagcct
     5581 gcgtaacggg ctctctctac accgggcttg cgggtgagat tgctctcatg gaacgccacc
     5641 agcagttcgg ctacggcgtt ctcggcctgc tgctctttcg gttgggctgg ggcttgtggg
     5701 gaggtcgata tgcgcgtttc ggcaactacc gcctggcccc tcgacgcctg attgagcact
     5761 ttcgcggcct cgaccacggc gctgcgcaca ccgcgcccgg gatggccttc accatcgtgt
     5821 tcctgctcgc gctggccgcg caggtcggca cgggtttgtt tgccactgac gacatcttca
     5881 cccaaggccc gttgagccgc ctcgtcgaga acgatacggc ccgcgcgctc acgggcatcc
     5941 accaccgggt gtactggctg atcctcgcgg ccctggccgc ccacctgaca gctcacgccg
     6001 tttacgcgct gcggcgtgac ccgatgccgc tggccatgat caccggtcgc aaacgcctcg
     6061 acctggagcc ggcgcgtgcg gcctggacgg cggcgttgct caccgcattg ggagccgctg
     6121 ctctggtctg ggcgatgctg gagctcgcct gagcgtcagt cggcgcggta gttgtcgtgg
     6181 cagcctttgc aggactgacc cagggcctga aaggcctggc cgagcgccgc atcgtcgttg
     6241 gatcgtgctg cagcgctgaa ggccgtggcc gcttcgcgca tggcttccac ccgcccggca
     6301 aaatcctcgg gttcttccca gatcgccggc agggcgtcgc cgccctcgga tccttcgggg
     6361 aacagcgtgc cgacgatgcc ggacagcgca gccagcgcat cggcgtggat ggtcatgtgg
     6421 gaggcgtgcg gcaccttctg cctcaagatg tccacggctg cctgcatgtg cccgccgatg
     6481 gcctccatgg tgtgctcgcg gtattcgagc tccccttcgc ttgcggtggc cgcgctggcg
     6541 gcacccagcg ccactaaccc gatcagccag tctcgacctc gcatgaactc tcctcatcgt
     6601 tgctggagtt gattgcgcgc accgtatttg aaatccggct ccgcggcaat tgccgccgtc
     6661 cgggagcggt gcagatccgg tcccttcttt ggcagtgttc agcgggccgg cccggtgata
     6721 gtttacgcca ccatgcctaa tgccccgtca cctctgccgg aagcgcatcc agcggtgcag
     6781 cccgtgccca acggcatgga ccgctttgcc atcacgctgt ccgggctctg cctcctccac
     6841 tgcctgttga ttccggtggt gctggccatg gcgcctgcat tttccagcct cgtgctcggc
     6901 acggagacaa ccgtgcactg ggtttttctg gggatggccg tgccgaccag cgtctgggcc
     6961 ctctggcgcg gctacggaag atgccagcac ctgcgagccc tggtgctggg catcgccggc
     7021 ctgctcgtga tgttcctggg cgtttctcac cttttcgatc cgcggctcga ggttccgctc
     7081 acccttgtcg gcgtcaccat ggtggtggtg gctcacgtgc tcaacctgcg aattcccgag
     7141 ccggctccgc gcgggtgact tggtttcgcc gaatcgacta tgatcggtcg gttcggatcg
     7201 agcataggga ggagaacgct catgtccacg tttcatgaca ttcgaatgga aagcatcgac
     7261 ggcgagcccg tggagctgtc caagtttcag aacaatctct gcctgatagt caacgtcgcg
     7321 agccgctgag gcctcacccc tcagtacgca ggtctgcgta cccttcagga acaatacggc
     7381 gaccggggtt ttaccgtgct cgccttcccg tgtaaccagt tcggtgccca ggagccaggc
     7441 tccaacgagg agatcctgga ttttgcgagc tcgaagttcg acgtgaactt cccgatgttc
     7501 ggcaagatcg aggtcaacgg ctcgggcgcg tgcgagctgt accaatatct caaagccgcc
     7561 cgtgcagacg aggaaggcaa tgcggacatc gcctggaact tcacgaagtt tctcgtggac
     7621 ggggacggga acgtcatcgc ccgtttcgcg ccgcccgtga cgccggagga gatcggcgag
     7681 cagatcgggt cgctgctgcc gggcgagcat gctcgggccc cggaccgtgc ggggcccaga
     7741 tttctcaggg ccgctccacg cgcagcaccc gaccttcgtc ggtgaccagg tagaggtgac
     7801 cctccgggcc cacccggatg tcccgcagcc gctcgcccag ttccccgaac agggcctcct
     7861 cctccacgac gcgatcgtcg cgcaggcgca ctcgccgcgc ttccctgtcc accagcgcgc
     7921 cgacgaacag gtcgccctgc cactcgggaa agacgtcgcc ccggtagacg gcgaggcctg
     7981 acggcgcgat ggaaggtacc caggagtgca gcggctgctc catgccgggc atctgctggt
     8041 aaggagagat gcgcgcgccg ctgtagtcga ggccgaacgt gatgaccggc cagccgtagt
     8101 tgcggccggg cctgaggaga ttgatctcgt cgccgccgcg aggaccgtgc tcgtggagaa
     8161 atacccggcc ggtcacgtga tccacggcca ggccctgcgg gttgcggtgc ccccaggtcc
     8221 acaccgccgg gtccgcgccc tcgcgtctca cgtagggatt gccatcggga atgcttccgt
     8281 catccctgat gcgaacggtt ttgccgagca gcccggaaag atcctgggct tcctcccggt
     8341 ggtcgaagcc gtccccggtg gtgaaaagca acgtcccgtc gggaaggaac aggaacttgc
     8401 cgccgtagtg ctggggcgtc gtcttgttcg ggcgggccgt aaagatcacc tctaagtcct
     8461 tcagcgagtt gccatccagg cggccgcggg cgatgcgcgt ggtgttggct cgcggcgtgc
     8521 cgtgggcata ggagatgtag atcagccggt tttcagcgaa gtccgggtgg ggggccacat
     8581 cgaacaggcc gccctgggcg cggaacaaca cctcggggat tccttccatg ggcgagccga
     8641 tctcgccctc gggcgagatc tgccgaaggc gaccgggacg ctccgtgacc agcaaccgcc
     8701 cgtcgggaag gaaggccacg caccacggca gctccaggcc ctcggcgacc tcgaccaggc
     8761 ggtagtcggc cgacattgcc tcggtggaga tggccagaga cagcgtcaga aagattagcg
     8821 ttgaaggctt tatggcgact tccggattga atgagggttc gaagggtgaa gcgaagccgc
     8881 tatgctagga catctgctca gagaggtgaa accattggcc gcgaccgtga tcgccagaat
     8941 cgcaggcgct tatctcctat ccgtgctggt cgcctacctc ctggccgcca cggccgctac
     9001 ccaggcctcc ctggctggcc tggaaggact gggcgtgacc atcacggtgg gtgagcggat
     9061 atccaccacg ggccaggacc tgatggggat gttgcccact tacgggctgc tgattgccat
     9121 cggttttgcc atcgcgcttc ccattgccgc cgccttcgcc cggttcattc cttcctggcg
     9181 ggccgtcggg ctcgtcgcgg caggagcggc cgcggtcggc ctcattcatg tcgggctgaa
     9241 ctcggtgttc gagacccacc tcgttgccgc gacccggacg acggtgggcc ttctggttca
     9301 ggtcatggcg ggtggcgtcg gcggctacgc tttctatcgg ctgcgggacc cgtgactgag
     9361 gcaggcaaag tcggctcgtg cagccgacga ctcctgagaa gagcagaagg accaggggag
     9421 cgcgggattc atgagggagc cgcacagctc agccagcggt cgcgggccgg ccgccatggg
     9481 tcggttcctg gatgcggtcg agcgcg
//
LOCUS       DBNHOX010000011         1593 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_65295, whole genome shotgun sequence.
ACCESSION   DBNHOX010000011 DBNHOX010000000
VERSION     DBNHOX010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_65295"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1593)
                     /gene="oadA"
                     /locus_tag="AC16UZ_00270"
     CDS             complement(<1..>1593)
                     /gene="oadA"
                     /locus_tag="AC16UZ_00270"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HIR6245138.1"
                     /translation="AHQSLLATRMRVEDMLPIAGKLDQVGFWSIESWGGATFDACIRY
                     LGEDPWERMRKLKAAMPNTPQQMLFRGQNILGYRHYADDVVGKFVERAAANGVDVFRI
                     FDALNDIRNLRTAIRATLDVGRHAQGTISYTVSPVHTMEVWVDLGKQIEDTGAHSIAI
                     KDMAGLLKPYVAFELVSRLKESVSVPIHMQCHATTGLSTAPYLKAIEAGIDNVDTAIS
                     SMSMTYGHSPTETLVSILEGGDRDTGLDISLLEEIAAYFRQVRKKYAKFEGTLKGVDS
                     RILVAQVPGGMLTNLESQLREQNASDRLDEVLDEIPRVREDLGFIPLVTPTSQIVGTQ
                     AVINVLMNERYKNVTKETAGVLKGEYGATPAPVNADLQARVLNGESPITGRPADHLQP
                     EVEALTGELQELAAERGFALAGDVVDDVLTYALFPQVGLKFLENRGNPDAFEPAPWVV
                     EVPALEAGRVVPAPPAPPRASTYNVRVNGRQFTVEVSEAGAVSSVKPAEPAFKAPVRA
                     GAGTIIAALAGNVFKVLVQPGD"
BASE COUNT          275 a          523 c          492 g          303 t
ORIGIN      
        1 cgtcccccgg ctgcacgagc accttgaaga cgttgcctgc aagcgctgcg atgatcgtac
       61 cggcacctgc ccgaacgggc gccttgaagg ccggttcggc cggcttgacg ctggacacgg
      121 cgcccgcttc ggaaacctcc acggtgaatt gcctgccgtt caccctcacg ttgtaggtgc
      181 ttgcccgagg gggtgcgggc ggcgccggca ccaccctgcc ggcttccaat gcaggcactt
      241 ccaccaccca gggcgcaggc tcgaacgcat cggggttgcc tcgattctcg agaaacttga
      301 gccccacctg gggaaacaaa gcgtaagtca ggacatcgtc cacgacgtcg cctgccagcg
      361 cgaaaccccg ttccgccgca agttcctgca gttcgcccgt caacgcttcc acctctggct
      421 gcagatgatc tgccggacga ccagtgatcg gcgactcccc gttgagcaca cgggcctgaa
      481 gatccgcgtt gaccggggcc ggcgttgcgc cgtattcccc cttgagcacg ccggccgtct
      541 ccttggtgac gttcttgtag cgctcgttca tcaggacgtt gatgaccgcc tgggtgccaa
      601 cgatctggga ggtgggcgta accagaggga tgaaaccgag atcctcacgg acacggggga
      661 tctcatcgag cacctcatcg agccggtccg aggcgttctg ctcgcgcagc tggctctcca
      721 ggttcgtcag catcccgcca ggcacctggg ctaccagaat gcgcgagtcc acgcccttga
      781 gcgtgccctc gaattttgcg tacttctttc ggacttggcg gaagtaggcg gcaatctcct
      841 ccagcagcga gatgtcgagc ccggtgtcgc gatcgccgcc ctcgagtatc gacaccagcg
      901 tctccgtagg cgagtgacca taggtcatgc tcatggacga gatggccgtg tccacgttgt
      961 cgatgccggc ctcgatggcc ttgaggtacg gggccgtcga cagccccgtc gtggcatggc
     1021 actgcatgtg gatgggcacg ctgaccgact ccttcaggcg gctcaccagc tcgaaggcca
     1081 catagggctt gagaagcccg gccatgtcct tgatggcgat ggagtgcgcg cccgtatctt
     1141 cgatctgctt gcccaggtcg acccagactt ccatggtgtg cacggggctg acggtgtacg
     1201 agatcgtccc ctgggcgtgc ctgcccacat cgagcgtcgc gcggatggcg gttcgcagat
     1261 tcctgatgtc gttgagcgca tcgaatatcc gaaaaacgtc gaccccgttg gccgccgcgc
     1321 gctcgacgaa tttgcccacg acgtcgtccg cataatgccg gtaacccaga atgttctggc
     1381 cgcggaagag catctgctgc ggtgtgttcg gcatggccgc tttaagcttg cgcatccgct
     1441 cccacggatc ttcacccaga taccggatgc aggcatcgaa ggtcgcgccg ccccaggatt
     1501 cgatggacca gaacccgacc tgatcgagct tgccggcaat cggcagcatg tcctcgacgc
     1561 gcatgcgggt agccagcagg ctctgatggg cgt
//
LOCUS       DBNHOX010000012         2721 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1543976, whole genome shotgun sequence.
ACCESSION   DBNHOX010000012 DBNHOX010000000
VERSION     DBNHOX010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1543976"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="AC16UZ_00275"
     CDS             <1..270
                     /locus_tag="AC16UZ_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245139.1"
                     /translation="ADRLSPRLKGSLDRGFFAQATRHLEDALQHTRASVSLRVDALHE
                     THLRHWRRLLHRLARHGDRVEIVLSEALGPRAQVDSSVFRLRFEG"
     gene            complement(466..1914)
                     /gene="cls"
                     /locus_tag="AC16UZ_00280"
     CDS             complement(466..1914)
                     /gene="cls"
                     /locus_tag="AC16UZ_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIR6245140.1"
                     /translation="MAVILSDAWPHIVAALIFALAVIASVHAILSRRDVRSTLGWVGV
                     IWLAPVLGPVLYSVFGINRIKRRATQLRAGGLQFTSDGQRTAEDLATTDQVSAAAHHL
                     VALETLVRRVTERSLTRGNSVQPLVNGEEAFPAMIGAIDAAERTVALSTYVFNRDAAG
                     EMFVAALARAVRRGVEVRVLIDAVGARYSRPPIAGTLRRRGIQVARFLRTALPWRMQY
                     MNMRNHRKLLVTDGRVGFTGGMNISEGNFVRRFKRAPVQDVHFCVTGPVVAHMMAVFA
                     EDWAFTAGEVLAGEDWFPPLEPVGQVLSRGLSDGPDIDIDKLPVVLTGALSCARESIR
                     IVTPYFLPDESMINALNVASLRGVDVRIVLPEVNNLPPVAWAATAQLPLMLERGCRFF
                     LSPGPFDHSKLLMVDEVWSLVGSANWDPRSFRLNFEFNLECYDRDLASHLARLADDKV
                     TRGRPVTREEVAGRGFATRLRDGIARLASPYL"
     gene            2333..2713
                     /locus_tag="AC16UZ_00285"
     CDS             2333..2713
                     /locus_tag="AC16UZ_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245141.1"
                     /translation="MKLSTLMLVNALVAAVFGVGFIVVPGQVMSAYGPEVTPQLEYVT
                     RLFGAALLAFAILTWTARNSPDSDTRRAILLALFVGASTGFIVSLIGQLGGVMNALGW
                     STVVIYLLLALGFGYFYAQPKETQ"
BASE COUNT          516 a          882 c          845 g          478 t
ORIGIN      
        1 gccgataggc tgtcgccccg gctgaagggg tcactggatc gcggcttctt cgcgcaggcc
       61 acccggcacc tggaagacgc gctgcaacac acacgggcct cggtgagcct gcgggtggac
      121 gcgctgcacg agacgcacct gcggcactgg cggcggttgc tgcaccggct ggcgcgccac
      181 ggggatcgtg tggaaatcgt tctgagcgag gccctgggtc cgcgggcgca ggtggattcg
      241 tcggtgttca ggctccggtt cgaggggtga acgcctgacc gcacgatgcg atccccggcg
      301 cagccggagt gcgacaccgt ccaaaacgcg aattggagca ggccggagtg agtccgtgac
      361 gtacagccgc aaacgccgga gttcatcgcg ctccagaccg tgatcgctgg cggttctcgc
      421 gggaacggaa agagcagacg atgctccgat cacaccgtcg tccggtcaca gatagggcga
      481 tgccagcctg gcgataccat cacggagtcg ggtagcgaaa ccacgccccg ccacctcctc
      541 ccgcgtgacc ggtcggcccc gtgtgacctt gtcgtcggcc aggcgggcca ggtggctggc
      601 gaggtcgcgg tcataacact cgagattgaa ctcgaagttc aggcgaaagc tcctcggatc
      661 ccagttggcc gagcccacca gggaccagac ctcgtccacc atcagcagtt ttgagtgatc
      721 gaacgggccg ggggacagga agaaccgaca cccacgttcc agcatcagcg ggagctgggc
      781 cgtggccgcc cacgcaaccg gtgggaggtt gttcacctcg ggtaacacga tgcggacgtc
      841 cacgcctcgg agggaagcca cgttgagcgc gttaatcatg ctttcatcag gcaagaaata
      901 gggcgtcacg atgcgaatgg attcccgggc acacgacaac gcgccggtaa gcaccactgg
      961 caacttgtca atatcgatgt ccgggccgtc ggagaggccg cgcgagagga cttgccccac
     1021 cggttcgagg gggggaaacc aatcctcacc cgccagcacc tctcccgccg tgaaggccca
     1081 gtcctctgca aacacggcca tcatgtgggc gacgaccgga cccgtcacgc aaaagtggac
     1141 gtcctgaacg ggcgcccgct tgaacctgcg aacgaagttg ccctcgctga tgttcatgcc
     1201 gccggtgaag ccgacacgcc cgtcggtcac gagcagcttg cggtgattgc gcatgttcat
     1261 gtactgcatc cgccatggca acgcggtgcg aaggaatcgc gcgacctgaa tcccccgtct
     1321 acggagcgtg ccggcaattg gcggtcgcga gtagcgcgcc ccgacggcgt cgatcagcac
     1381 ccgaacctcg accccgcgcc gaaccgcacg ggcaagagca gcgacgaaca tttcaccggc
     1441 ggcatcgcgg ttgaagacat atgtgcttag tgctacagtt ctttctgccg cgtcgatcgc
     1501 gccgatcatg gcggggaagg cctcctcgcc attcaccaat ggctgcacgc tgttcccgcg
     1561 cgtgagcgac cgttccgtca ctcgcctcac tagtgtctcc agcgcgacca gatggtgcgc
     1621 cgccgctgac acctggtccg tcgtggcgag gtcctcagcc gttcgctgcc catcgctcgt
     1681 aaactgcaag ccgcccgccc gcagttgcgt ggcccgacgc ttgatccggt tgatgccaaa
     1741 gacggagtac aataccggcc caagtaccgg agctagccag atgaccccga cccatccgag
     1801 tgtgcttcga acatcacgcc gagaaagaat ggcgtgcact gaggcgatca cggcgagggc
     1861 aaagatgagg gccgcgacga tgtgcggcca agcgtcgctc aggatcactg ccatctgcgg
     1921 aggtcgttcg ctccgatggc gctgacgagc cgggctgtcg tgagattagt gatcatgaag
     1981 atcgttctgc ccaacgttgt gatcggattc gtgagcagtg cagcatccga gcgaatcatt
     2041 gctctccata cccgcgagcg tctcgcaacg ctcaagcctc cgtcttgcga atccacttct
     2101 cgtgctgcaa gctaaggtgt cgccatcaaa ccatgacccc cccaccgcgc gggcgattgg
     2161 tggttaggat cagtgacgat ggcgtggtaa cattttgctc gacaagcgag ctacgccgac
     2221 ggcctccgtg ccccgctgcc atagttggac gccgcggcag acgcccgccc gacggcgagg
     2281 tcacagaacc tgcggccccg gttccaccct gcatacacgg aaggagctac ccatgaagct
     2341 cagcaccctc atgcttgtga acgctctggt ggcggcggtg tttggtgtcg gattcattgt
     2401 ggtccccggt caggtaatgt cggcatacgg tcctgaagtc acccctcaac tggagtatgt
     2461 gacgcgactg ttcggagccg cgctcctggc cttcgcgatc ctgacgtgga ctgccagaaa
     2521 ctcacccgac tccgatacgc gccgcgccat tctgctcgcc ctgttcgtcg gcgccagcac
     2581 tggcttcatc gtctccttga taggtcaact cggcggcgtc atgaatgccc tcggttggtc
     2641 aaccgtggtg atctacctcc tgcttgccct cggattcggg tacttctacg cacagccgaa
     2701 ggaaacccag taaagatggt c
//
LOCUS       DBNHOX010000013         5821 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_258265, whole genome shotgun sequence.
ACCESSION   DBNHOX010000013 DBNHOX010000000
VERSION     DBNHOX010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_258265"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /locus_tag="AC16UZ_00290"
     CDS             complement(<1..1696)
                     /locus_tag="AC16UZ_00290"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6245142.1"
                     /translation="MLDDLFSKKPLSLAVASAIAVSQPMAVAAATGQIEELVVTATKR
                     AESTQDIPVSVQAITGEDMKEAGVATFEEYIVYLPNVVRSGRGPGKNDLYIRGAATEQ
                     AGVTVSSANGTTPAVALYLDEQPVSFGGRNLDIYAADLQRVEVLPGPQGTLFGVSSQA
                     GTVRLITNKPNHEQFEAGFNAALSSTKGGDLSNSVEAYINVPVTDRLALRFVGYNDHQ
                     GGWIDNVEGTFTPSIDTINRAAIAFSPTFRPKIDPNASLQSTSNDLLVEDDFNDATYS
                     GGRVSASFDINDDWNLLLQHTAQSLDVEGVFEYDPHLDGKDSVNRYTPNKNQDDFGLT
                     TWTVEGRLGMLDVIYTGGYLDREVDATIDYTGYNNGGGYVAYYLCNFGEASYDTCFDP
                     TKQYAGDTDNERVTHEFRVTTPADNRWRVTAGVFIDDQETNTVGQFQYKSTGDAFRIP
                     IGQVGLDTRGANAGNVQFGPNTTFVNDFTRDQEQLAFFGEFAFDLTDAVTLSVGARYY
                     EIDFDFGGASNFSFGCKFRDGTDEFPDGPLNPDGSCNGTGYSNDVSERLEVLGTGDA"
     gene            2083..3063
                     /locus_tag="AC16UZ_00295"
     CDS             2083..3063
                     /locus_tag="AC16UZ_00295"
                     /inference="COORDINATES: protein motif:HMM:NF016974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HIR6245143.1"
                     /translation="MELSHPFIRLPFRFDADRLASELADIDDAAWLPHPSGMPGNSAV
                     ALVSRDGGDNDEFQGTMQPTGHLRRSPYMAQVMGSFGEILARSRLMRLSSKCEVQMHV
                     DFNYHWYTRVRIHIPIVTDPRVTFCCADERVHMAPGECWIFDSWRRHRVINASDSDRV
                     HLVVDMSGSSRFWRMVRHVQGSPDHAEEEFVAFDPGMQVSLSTERYNTAPVMAPGEVD
                     ALVKELVVDFSSHPDNDPGLVRRYALLLGDFCMDWRQIWLQHGYEREGIASYAALIDG
                     VQRQLHPNPRALVTSSNQVGVNPIIVQRILRPALAPEHLDDYLHDVRANG"
     gene            3133..3612
                     /locus_tag="AC16UZ_00300"
     CDS             3133..3612
                     /locus_tag="AC16UZ_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6245144.1"
                     /translation="MSEEEQVLITIRKIIRATDIYSRQLSKTVGLTVPQLLVLQAIRD
                     LGAVAISKISSEISLSQATVTSIIDRLEVRGLVSRRRSEADKRVVHAFLTEPGAELLD
                     SAPTPLQEVFRSRFDALEAWEKSMIIASLQRVAAMMDAEQLDASPMLAVGSPDPAAS"
     gene            complement(3609..4220)
                     /locus_tag="AC16UZ_00305"
     CDS             complement(3609..4220)
                     /locus_tag="AC16UZ_00305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6245145.1"
                     /translation="MGLYAKHVLPRMIDVLCGQPPMTELRRRYVPQASGDVLEIGIGS
                     GHNLAHYSSRVSSVTGLDPAAELTVMAQERANGLHTDVKLLQVSGEEIPADDARYDSL
                     VCTWTLCSIPNVYAAVREMRRVLKPGGRLYFVEHGASPDLPVARWQRRIEPVWKKIGG
                     GCHLTRRADELLQDAGFKLAELDTGYLPGPRFAAFMIHGIAER"
     gene            complement(4230..>5821)
                     /locus_tag="AC16UZ_00310"
     CDS             complement(4230..>5821)
                     /locus_tag="AC16UZ_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6245146.1"
                     /translation="PNEPRNRPMTHFPQRIAADRGDAPALTDERGSTSWAELNARSNQ
                     LVHAFRAMGLVPGDAIAIYAGNCREYYEVMMAANHAGLIYVPVNWHFSPEELAYVIDN
                     AGARLLVTEGQFLANAEAAVARGETPGLEQCVVIRPEADTTAFTDYEAFIGDQSTAEP
                     DDQGAGGPMFYTSGTTGRPKGVKSSTFAPGGPLAVLEMIGSGLSQMLQIPTDGTTYIC
                     GPVYHSAQWAFSFLPLISGSHVVMRHRFDPAESLAVMDAHEVTNVHLVPTQFHRLLRL
                     SDDERAGFSGSSLQVVWHGAAPCPPDVKRRMIQWWGPVVAEYYGSTEGSIVTTASASQ
                     WLERPGTVGKPSPLVEVTIRDDDGQPLPANESGQIYVKSLMGSDFEYHNEPAKTAEVH
                     LEPGTYTFGDIGYLDDDGYLYLSDRKIDMIISGGVNIYPAEIEAVLVNHPAVEDAAVF
                     GIPNDEFGEEVKAAIKLRPGQSGNEELSADIIGFCRDHLAHYKAPKSIDYEDEFPRHE
                     TGKLYKRLLRDRYWQDADRKI"
BASE COUNT          961 a         1763 c         1952 g         1145 t
ORIGIN      
        1 ctgcatcgcc cgtgcccagg acctccagtc gttccgacac gtcgttggag tagccggtgc
       61 cgttgcacga cccgtcagga ttgagcggcc cgtcggggaa ttcgtccgtg ccgtcccgga
      121 acttgcagcc aaaggagaag ttgctggcgc cgccgaagtc gaagtcgatc tcgtagtagc
      181 gggcacccac ggacagcgtc accgcatccg tgaggtcgaa ggcgaactcg ccgaagaagg
      241 ccagctgctc ctggtcgcga gtgaagtcgt tgacgaaggt ggtgttcggt ccgaactgaa
      301 cgttgccggc gttggctccc cgcgtgtcga gcccgacctg gccgatgggg attcggaagg
      361 catcgcccgt gctcttgtac tggaactggc cgacggtgtt ggtttcctga tcgtcgatga
      421 agacgccggc ggtcacgcgc cagcggttgt ctgcaggtgt ggtgacccgg aactcgtggg
      481 tgacgcgttc gttgtccgtg tctcccgcgt actgcttggt ggggtcgaaa caggtgtcgt
      541 agctcgcttc gccgaagttg cacaggtagt aggcgacgta gccgccgccg ttgttgtagc
      601 cggtgtagtc gatggtggcg tccacctcgc ggtcgaggta accgcccgtg tagatgacgt
      661 ccagcatgcc caggcgaccc tctacggtcc aggtggtgag cccgaagtcg tcctggttct
      721 tgttgggcgt gtaacggttg acggagtctt tgccgtccag atgcgggtcg tactcgaaca
      781 ccccttccac gtccagggac tgggccgtgt gctgcagcag caggttccag tcgtcgttga
      841 tgtcgaacga ggcgctcacc cggccgccgg agtaggtggc gtcgttgaag tcgtcctcga
      901 cgaggaggtc gttactggtg gactggaggc tggcgttggg gtctatttta ggacggaaag
      961 tgggagaaaa agcgatggcg gcgcggttta tggtgtcgat gctgggcgtg aaggtgccct
     1021 cgacgttgtc gatccagccg ccctgatggt cgttgtagcc gacgaagcgc agggccaggc
     1081 ggtcggtgac cggaacgttg atgtaggcct ccacggagtt gctcaggtcg ccgcccttgg
     1141 tgctggacag cgcggcgttg aagccggcct cgaactgctc gtggttgggc ttgttggtga
     1201 tcaggcgcac ggtgcctgcc tgggagctga ccccgaacag cgtcccctgg ggccctggca
     1261 gaacctccac ccgctgcagg tcggcggcgt agatgtccag atttctgccg ccgaaggaca
     1321 ccggctgctc gtccagatac agggccaccg cgggcgtcgt tccgttggcg gaggacacgg
     1381 taaccccggc ctgctccgtg gcggcgccgc ggatgtacag gtcgttcttg ccgggcccgc
     1441 ggccgctgcg caccacgttg ggcaggtaga cgatgtactc ctcgaacgtc gcaacaccgg
     1501 cctccttcat gtcctcgccg gtgatggcct ggacggatac cgggatgtcc tgagtggact
     1561 cggcgcgttt ggtggcggtg accacgagct cttcgatctg gccggtcgcc gcggcaaccg
     1621 ccatgggctg agataccgcg attgcggagg ctacggccag tgaaaggggt ttcttcgaaa
     1681 atagatcatc gagcatgtac atgtctctcc agtacgctga gtgggcctgc gtcgcgtcgt
     1741 caatacggct tgctatggtg gtcgcgctcg ttaaaggaac gcggaccggg cgcctatacg
     1801 gctggtatcg acgccaaatc gatgtgagtt aaatagatta gggccctaag tgtctatgtt
     1861 actcaaatcc gacgagaata cccattattt ctcaattatt ggggtttaag gccgcgtgac
     1921 tgcagatcta tatacaaatg aatcgaaata attatcagag tttgtcgtct gttttgtcgg
     1981 aaagttggta ggctgcgaag caaatttcag gtacggcctc agacaaggaa ggctcgcttg
     2041 ccggactcgg cgcggttcga cccggacggg attcgttgat ccatggaact ctcacaccca
     2101 ttcatccggc tgccattcag attcgacgcg gacaggctcg cctccgagtt ggccgacatc
     2161 gacgatgccg catggttgcc ccacccttct ggaatgcccg gcaattcagc cgtcgcgctc
     2221 gtttcccgcg acgggggtga caacgacgag ttccagggca cgatgcagcc gacggggcat
     2281 ctgcgtcggt ctccctacat ggcgcaggtc atgggatctt tcggcgagat cctcgcccga
     2341 tcacgcctga tgcgtctgtc ctcgaaatgc gaagtgcaga tgcacgtcga tttcaactat
     2401 cactggtaca cgcgggtccg gattcacatc cccatcgtta ccgatccgcg ggtgaccttc
     2461 tgttgtgctg atgaacgcgt tcacatggcg cccggcgagt gctggatatt cgattcgtgg
     2521 cgccgacatc gggtgatcaa cgccagcgac tccgaccgcg ttcacctcgt ggtggatatg
     2581 tctggttcct cgcggttctg gcggatggtc cgccatgtcc agggctcgcc ggatcacgcc
     2641 gaggaagagt tcgttgcttt cgatcctggc atgcaggtct cattgtccac ggagcgttac
     2701 aacaccgcgc cggtcatggc cccaggcgag gtcgacgccc tggtcaagga actcgtggtc
     2761 gatttttcgt cgcatcccga caacgatccg gggctggttc gacgctacgc gctgctcctc
     2821 ggcgatttct gcatggactg gcggcagatc tggcttcagc acggctacga gcgggaagga
     2881 attgccagct acgcggcgct gatcgacggc gtgcagcgcc agttgcaccc gaacccccgg
     2941 gcgctggtca cgagcagcaa tcaggtgggg gtcaatccca tcatcgttca gcggattctg
     3001 cgtccggcgc tggcaccgga gcatctcgac gattacctcc acgatgtgcg ggcgaacgga
     3061 taaccaaagc tcgccgtggc aggcgggcgt tggtaaggtt tcgctgtcca tcagtcgtgg
     3121 cccgggttgg agatgagcga agaagaacag gttctgataa cgatccggaa gatcatccgg
     3181 gcaaccgaca tctactcccg acagctgagc aagactgttg ggctcaccgt cccgcagttg
     3241 ctggtcctgc aggcgataag ggacctcggt gccgtggcga tttcgaagat atccagtgag
     3301 atcagcctga gtcaggccac cgtcacctct atcatcgacc ggcttgaagt ccgaggtctg
     3361 gtctcgcggc ggcgcagcga ggcggacaag cgggtcgtgc acgctttcct gacggagccc
     3421 ggggccgagc tgctcgattc cgcgccgacg ccgttgcagg aagtctttcg aagccgattc
     3481 gatgctctcg aagcctggga gaaatccatg atcatcgcgt cgctgcagcg ggtggcggcc
     3541 atgatggacg ccgagcagct cgacgcatca cccatgctgg ccgttggcag ccctgacccc
     3601 gcggcgagct agcgctcggc gatcccgtgg atcatgaacg ccgcgaagcg cgggcccgga
     3661 aggtagccgg tgtcgagctc ggccagcttg aagccggcgt cctgaagcag ctcatcggcg
     3721 cgtcgcgtca ggtggcagcc accgccgatc ttcttccaga ccggctcgat gcgtcgctgc
     3781 cagcgggcaa ccggcaggtc gggcgaggcg ccgtgctcga cgaagtacag gcgcccgcct
     3841 ggcttcagca cgcgacgcat ctcgcgcacg gccgcgtaga cgttggggat cgagcacagg
     3901 gtccaggtgc acaccaggga gtcgtagcgc gcgtcgtcgg ccggtatctc ctcgcctgag
     3961 acctgcagaa gcttgacgtc cgtgtgcagc ccgttggctc tctcctgagc catgaccgtc
     4021 agctcggcgg ccggatccag gcccgttacc gaggacaccc tggacgagta gtgcgccaga
     4081 ttgtggcccg atccgatgcc gatctcgagc acgtcgccgc tggcctgggg cacgtagcgg
     4141 cgcctgagct ccgtcatggg cggctggccg cagagaacgt cgatcatccg cgggagaacg
     4201 tgcttggcgt agagtcccat ggtcgttcct cagatcttcc ggtccgcgtc ctgccagtag
     4261 cggtcgcgca ggagccgctt gtagagctta cccgtctcgt ggcgcggaaa ctcgtcctcg
     4321 tagtcgatgg atttcggcgc tttgtagtgg gcgaggtggt cccggcagaa gccgatgatg
     4381 tcggcgctga gctcctcgtt gccgctctgt cccgggcgca gcttgatggc ggccttgacc
     4441 tcctcgccga actcatcgtt ggggatgccg aacacggccg cgtcttccac cgcgggatga
     4501 ttgaccagca cggcctcgat ttccgcgggg tagatgttga ccccgcccga gatgatcatg
     4561 tcgatcttgc ggtcgctcaa gtagaggtag ccgtcgtcgt ccaggtagcc gatgtcaccg
     4621 aaggtgtagg tgcctggctc cagatgcacc tcggcggtct tggccggctc gttgtggtac
     4681 tcgaagtcgg agcccatcag gcttttgacg tagatctgac cggactcgtt ggccggcagg
     4741 ggctggccgt cgtcgtcgcg gatggtcacc tccaccagtg gggacggctt gcccacggtg
     4801 cccggccgct ccagccactg cgaagcgctc gccgtggtca cgatggagcc ctcggtggag
     4861 ccgtaatact cggcgaccac gggcccccac cactgaatca tgcgtcgctt gacgtcgggc
     4921 gggcagggcg ccgcgccgtg ccagactacc tgcaggctcg atcccgagaa gccggcgcgc
     4981 tcgtcgtcgc tcaggcgcag cagccggtgg aattgggtcg gaacgagatg tacgttggtc
     5041 acctcgtggg cgtccatcac cgcaaggctc tcggccgggt cgaagcggtg gcgcatgacc
     5101 acgtgactgc cgctgatcag cggcagaaag gaaaacgccc actgcgcgga gtggtagacg
     5161 ggcccgcaga tgtaggtggt cccgtccgtg gggatctgca gcatctgcga caggcccgag
     5221 ccgatcatct ccaggacggc cagtggcccg ccgggggcga aggtgctcga cttcacgccc
     5281 ttgggtcgcc cggtggtccc cgaggtgtag aacatgggtc cgccggcgcc ctggtcgtcg
     5341 ggctccgccg tgctctggtc gccgatgaac gcctcgtagt cggtaaacgc cgtggtgtct
     5401 gcctcgggcc ggatgaccac gcactgctcg agccccggcg tctcgcccct ggccaccgcg
     5461 gcttcggcgt tggcgagaaa ctgcccctcg gtcacgagca ggcgcgcgcc ggcgttgtcg
     5521 atcacgtagg caagctcctc cggagaaaag tgccagttca cgggcacgta gatcaatccg
     5581 gcatggttgg cggccatcat cacctcgtag tactcgcggc agttgccggc gtagatggcg
     5641 atggcgtcgc ccgggacgag ccccatggcg cggaaggcat gaacgagctg gttgctccgg
     5701 gcgttcagct cggcccagga cgtgctgccc cgttcgtcgg tgagcgccgg ggcgtctccc
     5761 cggtcggcgg cgattcgttg cggaaagtgc gtcatcggtc ggttccgtgg ttcattggga
     5821 g
//
LOCUS       DBNHOX010000014         5597 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_65408, whole genome shotgun sequence.
ACCESSION   DBNHOX010000014 DBNHOX010000000
VERSION     DBNHOX010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_65408"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1231)
                     /locus_tag="AC16UZ_00315"
     CDS             complement(107..1231)
                     /locus_tag="AC16UZ_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6245147.1"
                     /translation="MTQRTMSQSAALVAVSVAFAVGIVGSLALRPPSGVGLNTPAAAT
                     TGETPLRIHWRMPVAFATNLPVLGDNALYVTGAIRAASNGALRIDPYEPNEIVPPFSI
                     TDAVRDRKVEAGYSWLGYDQGRIPASPLIGAVPFGMEPWEFSAWWFEAGGRELGEALY
                     AKQNTHILFCGISGPETAGWFRERIDSLEQMKGLKIRFAGLGGKVIEQIGASVTMLPG
                     GEIFQALEKGAIDATEYSLPIVDEKLGFNRIARYNYYPGWHQPSTAVHLIVNLDIWNA
                     LAYHDQQLIEMACTAGVTRNLARSEGLQGAVMARFPELGVSAETLPEEVLRELQRVSE
                     AVMLEQAEKDADFKKILESQQAFRSDYAIWKSRAYLPRNF"
     gene            1446..2573
                     /locus_tag="AC16UZ_00320"
     CDS             1446..2573
                     /locus_tag="AC16UZ_00320"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HIR6245148.1"
                     /translation="MLIGVPKETWPGEHRAALVPQSVKKLVGAGFEVVIEAGTGVPAG
                     YPDAAYVDAGATIGGDRAGLLASADVVLRVRKPEADEIAGLKKGSVHVSFLDPFNERG
                     LVDAMAAQGVTAISMEMIPRSTRAQKMDALSSQANLAGYVTVVLAANHCPKIFPMMMT
                     PAGTIAPSRVFVIGAGVAGLQAIATAKRLGARVEAFDTRPVVAEQVRSLGGKFVEIDL
                     GEVGQTEQGYAKELTPEQVELQKEGQKKVIGQSDVVITTAQVFGRPAPRIVSKDMVEA
                     MQPGSVVVDMAVETGGNVEGSVLNEVVDVGGVKIIGLGNLPSQVARHASDMYSSNLFN
                     LLDEFWSQEESKLDLDPEDEIVRACVITRDGSIVNETIKNL"
     gene            2586..2891
                     /locus_tag="AC16UZ_00325"
     CDS             2586..2891
                     /locus_tag="AC16UZ_00325"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha part 2"
                     /protein_id="HIR6245149.1"
                     /translation="MEAVFLAFVLMLSIFLGFELISKVPATLHTPLMSGANAISGITL
                     VGAITSAGAGADQIATLLGAVAVGFATINVVGGYLVTNRMLSMFKRKDTGGQSGGRS"
     gene            2888..4300
                     /locus_tag="AC16UZ_00330"
     CDS             2888..4300
                     /locus_tag="AC16UZ_00330"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR6245150.1"
                     /translation="MSLELINLWYIVAAALFIFGLKQLSSPDTAVRGNLLSSVGMLIA
                     VVVTLFSRDILEFQWIAAAAAVGAIIGAVAAQRVAMTSMPEMVALFNGSGGIASLLVG
                     WAALYLGDGAAAEAAVADTAVVTVGLSTFTIITILLSVLIGGLTFSGSLIAWGKLSEV
                     MTSRAIVFGAQRLVNAALLIGLVACGVMFAMEPSLTSPYLYAVIALSLIFGVMAVIPI
                     GGADMPVVISLLNSYSGLAACAAGFAINNNILIVAGSLVGAAGIILTNIMCKAMNRSL
                     ANVLFSGFGAVKTATKVEGEVKPITPDDAFLILEAASSVTFVPGYGMAVAQAQHVVRE
                     LGELLEENGAEVTYAIHPVAGRMPGHMNVLLAEANVPYDQLVEMDDINPRIDNIDVAV
                     VIGANDVVNPAAREDENSPIYGMPIINVDQARTVFVLKRSMASGFSGVDNPLFFGENT
                     RMLFGDAKASISSVIAEFKG"
     gene            complement(4424..>5597)
                     /locus_tag="AC16UZ_00335"
     CDS             complement(4424..>5597)
                     /locus_tag="AC16UZ_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6245151.1"
                     /translation="QETSVGATTAGDLRYGTLADAEGIRRLARWLAGSANPAIVVGDD
                     IAAEGAHRALITLAVQTGAAGWQQGLRAQISFPNVHPNSKGRLPFEAGAIRRALAGHD
                     LILLMGGPFFEEIWFDAENALPEEARVVHVESSHARLALNLAPDLGLVGNLRTTIEHL
                     VEEIAEGAPDDFGAAAKARNDALAESHRAAGDAAADRIERQGDTSPMTPARALLEISR
                     ALPKGAIVVDESITASLEVASRFDYAAPGDFFAGSGGGIGQGLAGALGVQVAQPNRRV
                     LAISGDGSAMYSIQALWSAVHHGLPIVFVILSNREYRILKHNLDIYRQRFDAGSNKPY
                     PHMDLTRPALGFPAMAEGMGMEGELVTRPEKIGSAIERAFAANAPYLVEVEISGKP"
BASE COUNT          957 a         1745 c         1833 g         1062 t
ORIGIN      
        1 ttgtgacgat gacgatcagc agcagcagcc acagccagga agcccattgg ccgatgcgaa
       61 cgagccaggc atccgcccac cgtgagaacg tggtttcagg cagcactcag aagttccttg
      121 gcaggtacgc gcgggacttc cagatggcgt agtcggagcg gaatgcctgt tgcgactcga
      181 gtatcttttt gaaatccgcg tccttctcgg cctgctcgag catgaccgct tcgctcaccc
      241 tttgaagttc ccgcagcacc tcttcgggaa gcgtttccgc ggagacgccg agttctggaa
      301 atctggccat gacggccccc tgcaacccct cggagcgggc gaggttgcgg gtgactcccg
      361 ccgtgcaagc catctcgatc agctgctggt cgtggtaggc cagggcgttc cagatatcca
      421 ggttgacgat cagatgcacg gccgtcgacg gctggtgcca gccgggatag tagttgtagc
      481 gcgcgatccg gttgaagccg agcttctcgt cgacgatggg cagcgagtat tcggttgcgt
      541 cgatggcgcc cttctctagc gcctgaaaga tctcgccgcc gggcagcatc gttacggacg
      601 cgccgatctg ctcgatgact ttgccgccga gacccgcaaa gcgaatcttc aggcccttca
      661 tctgctcgag ggaatcgatg cgctcgcgga accaacccgc cgtttccggc ccgctgatcc
      721 cgcagaaaag gatgtgggtg ttctgcttgg cgtacagcgc ttcgccgagc tcccgcccgc
      781 cggcctcgaa ccaccaggcg gagaactccc agggctccat gccgaagggg acagcgccga
      841 tgagcggtga cgcgggaatg cgtccctggt cgtaacccag ccaggagtag ccagcctcca
      901 cttttcggtc ccgcacggca tccgtgatgc tgaagggcgg cacgatctcg ttgggctcgt
      961 aggggtcgat gcgaagggca ccgttggaag ccgcgcgaat ggcgcccgtg acgtacaggg
     1021 cgttgtcgcc gagcacgggc aggttggtcg cgaaggcgac gggcatgcgc cagtggatgc
     1081 gcagcggggt ttcccccgtc gtagcggcgg cgggggtgtt cagcccgaca ccggatgggg
     1141 gccgcagcgc caggctcccg acgatgccca cggcgaacgc gacgctaacg gcgacgaggg
     1201 ctgcgctctg gctcatggtg cgctgagtca atctcaattc ccccgaagat tccgcggcat
     1261 tgcggatcca ttgtgggagg agcgtcaggc agacgcaagg cgatcggagc gcctcgcgcc
     1321 tccgagaatc ccgttaacat gtgaattgtt ttcgcgacgg ggtttggcta acttgctgcg
     1381 ctttcctgcg ccgcatcttc cgctctcttc ttcatgccgg cgcgactgcg atttgggggg
     1441 attgcatgct cattggggta cccaaggaaa cctggccggg ggaacatcgt gcggcgctcg
     1501 ttcctcagag cgtcaaaaag ctcgtaggtg ccgggttcga agtagtcata gaagccggga
     1561 cgggagtccc tgcgggttat cccgacgccg cctacgtcga tgccggtgcg accatcgggg
     1621 gcgatcgggc gggtctgctc gcctcggcgg acgtcgtcct gcgggtgcgc aagccggaag
     1681 cggacgagat cgccgggctc aaaaagggat cggtgcacgt cagctttctg gatcccttca
     1741 acgagagagg gctggtcgat gccatggcgg cccagggcgt cacggccatt tccatggaga
     1801 tgatcccgcg cagcacgcgc gcgcagaaga tggacgccct gtcatcccag gccaacctcg
     1861 ccggatacgt cacggtggtt ctcgccgcca atcattgccc caagatcttt cccatgatga
     1921 tgacgccggc cgggacgatt gccccgtcgc gggtgttcgt gatcggcgcg ggggtcgccg
     1981 gactgcaggc gatcgccacg gcgaagcggc tcggcgctag agtcgaagca ttcgataccc
     2041 gcccggtggt ggccgagcag gttcgctctc tcggcggcaa gttcgtcgag atcgatcttg
     2101 gcgaggtcgg gcagacagag cagggctatg caaaagagct gacgcctgag caggtggagc
     2161 tccagaaaga aggtcagaaa aaggtgatcg gccagtccga cgtggtcatc accaccgcgc
     2221 aggtgtttgg tcgtccggca ccccgcatcg tgtcaaagga catggtcgag gccatgcagc
     2281 cgggaagcgt cgtggtcgac atggccgtcg agaccggcgg caacgtcgag ggctctgttc
     2341 tcaacgaagt ggtcgacgtc ggcggcgtga agatcattgg cctgggcaat ttgccgtcgc
     2401 aggttgcgcg gcatgccagt gacatgtact cgagcaatct cttcaacctg ctggatgaat
     2461 tctggagcca ggaggaaagc aagctcgacc tcgatccgga agacgaaatc gttcgggcct
     2521 gcgtgatcac gcgggacgga tcgatcgtca acgaaaccat caagaacctc taggagtccg
     2581 gaaccgtgga agccgttttc ctcgccttcg tattgatgct ctccatcttc ctgggcttcg
     2641 agctcatttc caaagtgcct gcgacgctgc acacgcctct gatgtcgggc gcgaacgcga
     2701 tctcgggcat caccctcgtc ggcgcgatca catccgcggg cgcgggggcc gaccagatcg
     2761 cgacgctgct gggcgctgtc gccgtcggtt ttgccaccat caacgtggtg ggcggttacc
     2821 tcgtcaccaa tcgcatgttg tccatgttca agaggaaaga caccggtggt cagtcggggg
     2881 gtcggtcatg agcctggaac tgatcaacct ctggtacatc gtcgccgccg cgcttttcat
     2941 attcggcctc aagcagctca gctcgccgga cacggccgtg cgtggcaacc tgctgtcatc
     3001 cgtgggcatg ctcattgccg tggtggtgac cctgttctcc cgggatatcc tcgagttcca
     3061 gtggattgcg gcggctgctg ccgttggcgc gatcatcggg gccgttgccg cgcagagagt
     3121 ggccatgacg agcatgccgg agatggtcgc cctgtttaac ggctcgggag gcattgccag
     3181 cctgctggtt ggctgggcgg ccctctacct cggcgatggc gccgccgccg aggccgcagt
     3241 ggccgacacc gccgtcgtca cggtgggtct ttctaccttt accatcatca ccatcctgct
     3301 ctccgtgctg attggcggct tgaccttctc gggcagcctc atcgcctggg gcaagctgtc
     3361 agaggtgatg acgagccggg ccatcgtctt cggggctcag cgcctggtca acgccgctct
     3421 gctgatcggt ctcgttgcct gtggcgtgat gttcgccatg gagccgtcgc tgacctcacc
     3481 ctatctctac gcggtaatcg cgctgtcgct catcttcggc gtaatggcgg tcattcccat
     3541 cggtggcgcc gatatgccgg tggtgatctc gctgctcaac agctactccg gtcttgccgc
     3601 atgcgcggcg ggatttgcga tcaacaacaa catcctcatc gtcgcgggtt cgctggtggg
     3661 cgctgcgggc atcatcctga ccaacatcat gtgcaaggcc atgaaccgtt cccttgccaa
     3721 cgtgcttttc tccggcttcg gtgccgtcaa gaccgccacc aaggtcgagg gcgaggtcaa
     3781 gccgatcacg ccggacgacg cgttcctcat tctggaagca gcctcatcgg ttacgttcgt
     3841 cccgggttac ggaatggccg ttgcccaggc gcagcacgtg gttcgggagc ttggtgagct
     3901 tctcgaggaa aacggtgccg aggtgaccta cgccatccat ccggtggccg ggcggatgcc
     3961 ggggcacatg aacgtcctgc tggccgaggc gaacgtgcct tacgatcagc tggtcgagat
     4021 ggatgacatc aacccccgca tcgacaacat cgacgttgcg gtcgtgatcg gcgccaacga
     4081 cgtggtcaat ccggctgccc gggaggacga gaacagcccg atctacggca tgcccatcat
     4141 caacgtcgac caggcgcgaa ccgtgttcgt cctcaagcga tcgatggctt ccgggttctc
     4201 gggcgtggac aacccgctgt tcttcggcga gaacacgcgg atgctgttcg gcgacgccaa
     4261 ggcgtcgatc tcctcggtga tcgccgagtt caaagggtag ccgacaggcc tttcagggcg
     4321 tagacacgcc cgacaccgcc gagatgaacc catcgaggtt gggcatcgtg atacccggca
     4381 gcgtggtccg gatcgcgttg cgctggaggg ttctctgcca acgctagggc tttccggaga
     4441 tctcgacctc caccagataa ggcgcgtttg cggcgaacgc gcgctcgatg gcggagccga
     4501 tcttctccgg ccgtgtgacc agctctccct ccattcccat tccctcggcc atggcgggaa
     4561 aacccagtgc cggtctggtg agatccatgt gcgggtacgg cttgttggac cctgcatcga
     4621 agcgttggcg gtaaatgtcg agattgtgtt tgagaatccg atattcacgg ttcgacagga
     4681 tcacgaacac gatgggcagc ccgtgatgca ccgcggacca cagggcctga atgctgtaca
     4741 tggcagagcc gtctccggag atcgccagga cgcgccgatt gggctgagcc acctgaacgc
     4801 ccagggcgcc ggccaggccc tggccgatac cgccgcccga gcccgcgaag aagtcgccgg
     4861 gggcggcgta atcgaatcgc gatgccactt ccaacgatgc ggtgatggat tcgtccacga
     4921 cgatggcgcc tttgggcagc gcgcggctga tttccagcaa ggccctggct ggcgtcatcg
     4981 gcgaggtatc gccctggcgc tcgatccgat ccgcggcggc atcgccggcg gcccgatggg
     5041 attcggccag cgcgtcgttt cgcgcctttg ccgccgcccc gaagtcgtcg ggcgcgccct
     5101 cggcgatttc ctccaccaga tgctcgatcg ttgtccgcag gttgccgacc agaccgagat
     5161 cgggggccag gttcagtgcc agacgggcat gactggactc cacgtgaacg acccgggcct
     5221 cctcgggcaa ggcgttttcc gcatcgaacc agatctcctc gaagaacggg ccgcccatca
     5281 gcagaatcag atcatggccg gcgagcgcgc ggcgaatggc gccggcttcg aacggcaggc
     5341 gtcccttgct gttggggtgc acgttcggga aggaaatctg agcccgcaaa ccctgctgcc
     5401 atccggcggc gccggtctgc acggcgagtg tgatcagcgc ccggtgggca ccctcggcgg
     5461 cgatgtcgtc gccgacgacg atggctgggt ttgcgctccc ggccagccag cgcgccagtc
     5521 ggcggatgcc ctcggcgtcc gcgagcgtcc cgtagcggag atcccctgcg gtagtggcgc
     5581 caacgctggt ttcctgc
//
LOCUS       DBNHOX010000015         5667 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1256, whole genome shotgun sequence.
ACCESSION   DBNHOX010000015 DBNHOX010000000
VERSION     DBNHOX010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1256"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /gene="amrB"
                     /locus_tag="AC16UZ_00340"
     CDS             <1..857
                     /gene="amrB"
                     /locus_tag="AC16UZ_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HIR6245152.1"
                     /translation="LIHGCFMGSVPPYTKRLNNLPNAMVSRSPAVADLFYPGDPAELA
                     GTVRAYLDRADAPTVALRPKALIVPHAGYAYSGPVAATAYRTLEPFRHAVERVILMGP
                     AHRVYLEGLAVPETDAFAVPTGNVPVDVEAVARLLELPGVLASDQAHAAEHCLEVHLP
                     FLQAVLDRFSIVPVVVGACSSELVADVLDLLWGGDETLIVVSSDLSHYHPYEVAQALD
                     AATAGEILARACNLTGEQACGAVAINGLMLAATGHDLQVRELDLRNSGDTAGDRDRVV
                     GYGAFALD"
     gene            835..1470
                     /gene="amrA"
                     /locus_tag="AC16UZ_00345"
     CDS             835..1470
                     /gene="amrA"
                     /locus_tag="AC16UZ_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HIR6245153.1"
                     /translation="MARLPSTDYGPDARQALLGIARDAIRIGLERHGVADPMSVVDVP
                     SLLPELLEPRATFVTLTQAGSLRGCTGSLEALRPLALDVVENACHSAFRDPRFPPVQA
                     GELNRLRIELSVLSPLERMRVASEGELLERLEPSMGLVIEEGGRRGTFLPKVWESLPD
                     PRVFLDELKAKAGFPAGHWSDATEVFYYRTQTFAEPVAAVGSVVSHGGDSV"
     gene            1495..2673
                     /locus_tag="AC16UZ_00350"
     CDS             1495..2673
                     /locus_tag="AC16UZ_00350"
                     /inference="COORDINATES: protein motif:HMM:NF047238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR6245154.1"
                     /translation="MNRSLRGRVAVVGVGETMYYKAGQSPDAEFKLALKAILAACEDA
                     GIDPKDVDGFASYSNDRSDPSRLAAALGCRELRFANMQWGGGGGGGSGALGNAAAAVA
                     TGMADCVVVFRALAQGQFGRFGQGPRRNVIAGDMAHVVPYGLMSPAQMFAMKVQRFMH
                     DHGVEQSALRAISLASYHHAQNNPRAVMHGRPLDEATYDASRWIVEPFHLYDCCLEND
                     GAAAMIVVPADRAGDCRPVPAYLLGAVSGSHYRAGASVHNTPDYATSTFKTLAPRLYE
                     MAGVVPADVDVLQSYENFTGGVLMSIVEHGFCEAQRCNEFFVPERLTAPDGDLPLNTS
                     GGNLAECYMHGLGLNIEAVRQIRGESTNQVMGAEISMVISGPMVTPVSACILGSEAAL
                     "
     gene            2670..3113
                     /locus_tag="AC16UZ_00355"
     CDS             2670..3113
                     /locus_tag="AC16UZ_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6245155.1"
                     /translation="MSYYLRPGLPQPAPLPDGLDRPYWDGLREDRLMLQRCAQCGGWQ
                     WGPEWICHHCLGFELAFEAVTPRGIVYSYERVWHPVHPALAEQGPYLVVLVEIPQADG
                     VRLVGNLLGDPLQDVEIGSAVEAVFEHHEDVDEPFTLLQWRLLAD"
     gene            3140..4300
                     /locus_tag="AC16UZ_00360"
     CDS             3140..4300
                     /locus_tag="AC16UZ_00360"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6245156.1"
                     /translation="MIEIHGYCDERFSPVREVFARNFADKDEVGASLALTVEGEMVVD
                     MWAGHRDAAKSLPWEQDTIVNVWSTTKTMSALCALVLADRGEIDLYAPVTRYWPAFGQ
                     NGKEKIEVRHFMSHSAGLAGTDEPIRGEALYDWERVVNALERQAPWWEPGTASGYHAI
                     TQGHLIGEVVRRVTGMSCGTFFRREIAEPLGADFHIGTAPEHFSRIGDLLPPGVSLGG
                     ALAHEPSGRGGGEGRAASIAVRAFRSPFVRAEDAATAEWREAEIPAANGHGNARSVAR
                     IQTVIANGGVSAGTRILSEAGCRAVFDEQTHGRDLVLKAPLTFGMGYALLHGQSPREL
                     AFWGGWGGSSVVVAPQSRMCIAYVMNRMEPSLLGDDRGVSLNRAALAVMGRG"
     gene            complement(4303..5160)
                     /locus_tag="AC16UZ_00365"
     CDS             complement(4303..5160)
                     /locus_tag="AC16UZ_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010562866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HIR6245157.1"
                     /translation="MSINIGKFEYPAHPKLTAHPTLWKVLPDARELVTANVERLSELQ
                     ERLYANNRYSVLMIFQGMDAAGKDSTIKYVTSGVNPMGFQVVNFKQPTYKELDHNYLW
                     RYWRAMPERGRIGIFNRSHYEEVGVARVHPEIIERQNLPSEHVNGDFWENRFEDIRAM
                     ERHLHTNGTIVLKFFLNVSKKEQKARLMKRLTNRDKHWKFDPRDIDERVHWDDHMKAY
                     EAAIAATHFDHAPWYVIPADDKWTMRVIVAEVLCTHLEQLRLSYPPAKGAHKHALAEA
                     LSRLEAEKE"
     gene            5407..>5667
                     /locus_tag="AC16UZ_00370"
     CDS             5407..>5667
                     /locus_tag="AC16UZ_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245158.1"
                     /translation="MDEAYRLVFRGEFLEGQHPAVARKRLVQALQLNDAQAEKLFSGH
                     PVVVKKRADAATAARYQDLFKQAGARLRVMPLASEAPAAEETT"
BASE COUNT          895 a         1756 c         1914 g         1102 t
ORIGIN      
        1 gtctgatcca tgggtgcttt atggggtcgg tacccccata taccaagcgc cttaacaatc
       61 ttccgaatgc catggtcagc agatcgcccg ccgtagcgga cctgttctat cccggagatc
      121 cggccgagct ggccgggacg gtgcgggcgt acctggaccg cgcggatgcg cccacggtcg
      181 ccctgcgacc gaaggcgctg atcgtgcctc acgccggcta cgcatactcc gggccggtgg
      241 cggcgacggc gtatcggacc ctcgagcctt ttcggcatgc ggtggaacgt gtgatcctca
      301 tgggcccggc ccatcgggtg tatctggaag gcctggcggt ccccgagact gatgcgtttg
      361 cggtcccgac gggtaacgtg ccggtggacg tcgaagccgt ggcccgactg ctcgagctcc
      421 ccggagtgct ggcctctgac caggcccacg ccgccgagca ctgcctggag gtgcacctgc
      481 ccttcctcca ggcggtgctt gatcgtttct ccatcgttcc cgtggtcgtc ggcgcgtgct
      541 cgagcgagct ggtggccgac gtgctcgacc tgctctgggg cggagacgaa accctcatcg
      601 tcgtgagttc cgatctgtcc cactatcatc cctacgaggt ggctcaggcg ctcgatgcgg
      661 cgacggcagg cgagattctc gcgcgcgcct gcaacctgac cggcgagcag gcgtgcggag
      721 cggtcgccat caacggtttg atgctggcgg ctaccggtca cgaccttcag gtgcgggagc
      781 tcgaccttcg caattcgggc gatacggccg gcgaccgtga tcgggtggtg ggttatggcg
      841 cgtttgccct cgactgatta cggccccgac gcgcgacagg ccctgctggg catcgcgcgg
      901 gacgccattc ggattggtct cgaacgccat ggcgtcgcgg acccgatgtc cgtggtggac
      961 gttcccagcc tgcttcccga gcttctcgag cctcgagcga cgttcgtcac cctgacccaa
     1021 gccgggtcgt tgcgcggttg cacgggttcg ctggaggcgt tgaggccgct cgcgctcgat
     1081 gtggtggaaa acgcctgcca ctccgcattt cgcgacccgc gcttccctcc ggttcaggca
     1141 ggcgagctga accgtctgcg catcgagctt tcggttctct cccccctgga gcggatgcgg
     1201 gtagccagcg agggggagct cctggagcgt ctcgagccgt ccatgggtct ggtgatcgag
     1261 gaaggcggcc ggcgcggtac ctttctgccc aaggtctggg aaagcctgcc ggatccacgc
     1321 gttttcctgg acgagctcaa ggccaaagcc ggcttcccgg ctggccactg gtcggatgcc
     1381 acagaggttt tctactaccg aacccagacg tttgccgagc cggtggcagc ggtcggttcc
     1441 gtggtgtcgc atggcggtga ttcggtgtag attccccggg atgggggaaa ccagatgaat
     1501 cgctcgctca ggggccgggt cgccgtcgtc ggcgtgggcg agaccatgta ctacaaggcc
     1561 ggccaatcgc cggatgccga gttcaagctc gcgctcaagg cgatcctcgc ggcctgcgag
     1621 gatgcaggca tcgacccgaa ggacgtcgat ggtttcgctt cctacagcaa cgaccgcagc
     1681 gacccgtcgc ggctggccgc agcgctgggt tgtcgtgaac tccgctttgc caacatgcag
     1741 tggggcggtg gcggaggggg cggctccggg gcgctcggca acgccgcggc cgccgttgcc
     1801 actggcatgg ccgattgcgt ggtggttttt cgcgcgctcg cccaggggca gttcggccgg
     1861 ttcgggcagg gcccgcgacg caacgtcatc gccggtgaca tggcccacgt ggtgccgtac
     1921 ggcctgatgt cgccagctca gatgttcgcg atgaaggtgc agcgattcat gcacgaccac
     1981 ggtgttgagc agtcggcgct gcgggcgatc tccttggcgt cttaccacca tgctcagaac
     2041 aacccccgtg cggtcatgca cggacggcct ctggatgagg cgacttacga tgcgtctcga
     2101 tggatcgtcg agccgtttca tctctacgat tgctgtctcg agaacgacgg cgcggctgcc
     2161 atgatcgtgg tgccggcgga tcgtgcaggc gactgccgcc cggttcctgc ctatctgctt
     2221 ggtgccgtgt ccggttccca ctatcgggcc ggggcgtcgg tgcacaacac gcccgactac
     2281 gcgacgagca cgttcaagac gctggctccc cgcctgtacg agatggccgg ggtggtcccc
     2341 gccgacgtcg acgtgctgca gagctacgag aacttcactg gcggcgtgct catgagcatc
     2401 gtcgagcacg gattctgcga ggcgcaacgg tgcaacgagt tcttcgtccc ggagcggttg
     2461 acggcgcccg atggggacct tccgctgaat accagcggcg gaaatctcgc cgagtgctac
     2521 atgcacggtc tggggctcaa catcgaagcg gttaggcaga ttcgcggtga gtccaccaat
     2581 caggtgatgg gcgcagagat atccatggtg atctcgggcc ccatggtcac cccggtgagc
     2641 gcgtgcatcc tgggttcgga ggcggcgcta tgagctacta cctcagaccc ggcctgccac
     2701 agcccgcgcc gcttccggac ggcctggacc gaccttactg ggacgggctg cgcgaggatc
     2761 ggctgatgct gcagcggtgc gcgcagtgcg gtggctggca gtggggtccc gagtggatct
     2821 gccaccactg tctcggcttt gagcttgctt tcgaagcggt aacaccacgc ggtatcgtct
     2881 acagctacga acgggtctgg cacccggtcc atcccgcgct tgccgagcag ggcccttacc
     2941 tggtcgttct ggtcgagatt ccccaggcgg acggggtgcg attggtcggc aacctgctcg
     3001 gcgacccgct ccaggatgtg gagatcggct cggccgtgga ggccgtgttc gagcatcacg
     3061 aggacgtgga cgaacccttc acgctgctgc agtggaggct tctggccgac taattctgcc
     3121 cttgaactcg gagttcagca tgatcgaaat ccacggctac tgtgacgagc gcttctcgcc
     3181 cgtgcgtgag gtctttgccc gaaactttgc cgacaaggac gaggttgggg cttcgctcgc
     3241 cctgacggtt gagggcgaga tggtcgtcga catgtgggcc ggccaccggg atgccgcaaa
     3301 gtctctgccc tgggagcagg acaccatcgt caacgtgtgg tcgaccacca agaccatgtc
     3361 agcgctttgc gccctggtgc tcgccgatcg cggcgagatc gatctttacg ccccggtcac
     3421 ccggtactgg ccggcgttcg gccagaacgg caaagagaag atcgaggtaa gacacttcat
     3481 gagccactcc gccggccttg ccggcaccga tgagccgatt cgtggtgaag ctctctacga
     3541 ctgggagcgg gtcgtcaacg cgcttgaaag gcaggcgccc tggtgggaac ccggaacggc
     3601 gagcggatac cacgcgatca ctcagggaca cctcatcggc gaggtcgtgc gccgcgtgac
     3661 gggtatgtcc tgcggcacgt tcttccggcg cgagatagct gagcctctgg gcgccgactt
     3721 tcacatcggc accgctcccg aacacttctc gcgcattggc gacctgctgc cgccgggtgt
     3781 gagcctcggc ggggctcttg cgcatgagcc ctcagggaga ggtggaggcg agggccgagc
     3841 ggccagcatc gcggtgcgcg cgttccgcag cccgttcgtg cgggcggaag atgcggcgac
     3901 cgcggaatgg cgcgaagcgg agatcccggc cgccaacggt cacggcaacg cgcgatccgt
     3961 ggcccgcatc cagacggtga tcgccaatgg cggcgtgtct gctggaactc ggatcctgtc
     4021 cgaggctggg tgccgggcgg ttttcgacga gcagacccac ggccgcgacc tcgttctcaa
     4081 agcgccgctc acgttcggta tgggatatgc cctgttacac ggacaatctc caagggaact
     4141 cgcgttctgg ggcggctggg gcgggtcctc ggtggtcgtc gctcctcagt cgcgcatgtg
     4201 catcgcttac gtgatgaatc gcatggaacc gagccttctg ggcgatgatc gtggggtgtc
     4261 cttgaaccgc gcggcgcttg ccgtcatggg ccgcggctag gctcactcct tctcggcttc
     4321 aagacgtgat agcgcctcgg ccagcgcgtg cttgtgcgcg cccttggccg gcgggtagct
     4381 gagcctgagc tgctcgaggt gcgtgcagag cacctcggcg acgatgacgc gcatggtcca
     4441 tttgtcgtcc gccggaatca cgtaccaggg cgcgtgatcg aagtgggtgg ccgcgatggc
     4501 ggcctcgtag gctttcatgt ggtcgtccca gtgcacgcgc tcgtcgatgt cgcgggggtc
     4561 gaacttccag tgcttgtcgc ggttcgtcag gcgcttcatc aggcgcgctt tctgctcttt
     4621 cttggaaacg ttcagaaaga acttcaatac gatggtgccg ttggtgtgca ggtgccgttc
     4681 catggcacgg atatcctcga agcggttctc ccagaaatcg ccgttgacat gctcgctggg
     4741 cagattctgc cgctcgatga tctcggggtg aacgcgggcg actccgacct cttcgtagtg
     4801 cgaacggttg aagatgccga tgcggcctcg ctccggcatg gcgcgccagt agcgccacag
     4861 gtagttgtgg tcgagttcct tgtaggtggg ctgcttgaag ttgaccactt gaaagcccat
     4921 gggattgacg ccgctggtga cgtacttgat ggtgctgtcc ttccccgctg cgtccatgcc
     4981 ctggaagatc atgagaaccg aatagcggtt gttggcgtac agtcgctcct gcagctcgga
     5041 gagccgctcg acgttcgcgg tgaccagctc ccttgcgtct ggcagcactt tccacagcgt
     5101 ggggtgcgct gtcagcttgg ggtgcgctgg atactcgaat ttcccgatgt tgatgctcat
     5161 ctggagtcca atgtgccttt gcgcgcgcgg cgcccgcttg cgccgttcga gataccttaa
     5221 ccagcgccat gagggacgtc gagtaacaaa cgcacgggtg cccacggctc aacgccgata
     5281 tctgggacgc ttttaccaat cactggcgcg acttgtgcgc ccgtgcccat tttccgcttg
     5341 cgagaagtgt gaacatgcgg tcattgcgcc gaagtagatg tggcgcacag ttctagaatg
     5401 ctcatgatgg acgaagctta ccgattggtg ttccgcggcg agttcctgga aggacagcac
     5461 cccgcggtgg ccaggaaacg cctggtgcag gcgctccagc tgaacgacgc gcaggcggag
     5521 aagctcttca gcggtcaccc ggtggtggtc aagaagcgcg cggacgcggc tactgcagca
     5581 cgctatcagg acctgttcaa acaggccggc gccagattgc gcgtgatgcc gctggcctcg
     5641 gaagcaccgg cggccgaaga aaccacg
//
LOCUS       DBNHOX010000016         3529 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1415547, whole genome shotgun sequence.
ACCESSION   DBNHOX010000016 DBNHOX010000000
VERSION     DBNHOX010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1415547"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="AC16UZ_00375"
     CDS             complement(<1..828)
                     /locus_tag="AC16UZ_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245159.1"
                     /translation="MAETALTARDFEPVHAERRIQTISRPSLSYWQDAWIRLKANRRA
                     LFSLYIVLGLLAFTIAGPFVWTVDPAAQDVDQISQPPGTDRRAAVVKPYQPWDGSSVL
                     PLTEGLRLAEPATNQAVRLMWDATPGAAGYVIYRNLFPVGPENALGLPLGENFSAEDT
                     KFEDLLDLRPRRYYYSVVSLGAAGNELAQETLMVNVDRVITVAEAEARGMGEGRQLAV
                     GDIVRLPLHPLGTDYLGRDLLARLMEGARVSLFIGICAPFLYVLFGVAYGSAAGFLGG
                     "
     gene            complement(832..1764)
                     /locus_tag="AC16UZ_00380"
     CDS             complement(832..1764)
                     /locus_tag="AC16UZ_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6245160.1"
                     /translation="MLNYTLKRLLQGIVTVWFIATATFIAMHSVPGDPLADEKAVTAE
                     IRANLEAKYGLDKPVFDQYLIYMGNIVKGDFGISFTQENREVNDIIREHFPVSATLGI
                     LAVVFAGLGGVLWGALTALYRNRLPDVIIMFLVILGISVPSFVIAAISQLSLVNLNDL
                     LGVSILPVAGWGSVWHMLVPSMVLGLGTMAYLTRLMRSSMLEIVNADYVRTAKSKGLP
                     PTRIFTRHQLRNAILPVITVLGPSIAAITTGGFVVELVFAIPGLGRYFVQAVQQLDYT
                     VIMGTTVFYGSFLVFMVIVVDLLYGLIDPRVRLE"
     gene            complement(1775..3469)
                     /locus_tag="AC16UZ_00385"
     CDS             complement(1775..3469)
                     /locus_tag="AC16UZ_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6245161.1"
                     /translation="MTTVAGSEDTFNRSAGRQLLLFAAISIGSIGLLMYALVWASSLT
                     GSGAGSEVAVDPETRTVTLFLREEPPQLNSSLATDMVSGMVLGHVMEGLLRYDEHNRL
                     VPGMAERWEIDANGAIFHLRSDAVWSDGAPVTAHDFVFAWQLALDPANASQYAFILYP
                     VKNGRAIAEAKLPVSQLGARAVDDRTLEVTFERPVAYFDKLVAFPTYYPIRRDFYEAT
                     KGRYGADAHTLLYNGPFRMTRWVHGAHVRLEKNPTYWDEERIRIEVLDFPYITSDANA
                     TLNLFKDNKVAYAGLLAENLNDAMEQRWHIKRFMDGSVFFIEFNFRDGRVTANTNLRK
                     AMQHAVDPAELVYKVTKLPGYIPGESLFPVWLRGVNGYFRQEYPAPKTIKDVVKAREY
                     LQAAKRELGMEQIPPLVLLSGDNPVSNIQSEYFQQVFKQTLGLDIRIDKQIFKQRLAK
                     MTSGDFDMVLAGWGPDYDDPLTFGDLFASWNLNNRGRYSNPELDRQVAIAEESLDPKT
                     RMDAFGEIQRILHDDVVILINYEPGTTYVVNPDLGGLVRRSVGPDPDFTNAYIVEG"
BASE COUNT          619 a         1091 c         1214 g          605 t
ORIGIN      
        1 cccgccgaga aagcccgccg cgctgccgta ggccaccccg aacagcacgt agaggaacgg
       61 cgcgcagatg ccgatgaaga gggacaccct cgcgccctcc atgagccggg ccaggaggtc
      121 gcggcccaga tagtccgtgc ccagaggatg caggggcagc ctcacgatgt cgccgacggc
      181 cagctggcgg ccctcaccca tgccccgggc ctcggcttcc gctacggtga tgacccggtc
      241 gacgttgacc atcagcgtct cctgagccag ctcgttgccg gcggcgccca ggctgacgac
      301 gctgtagtag tagcggcgcg ggcgaagatc cagcagatcc tcgaacttcg tgtcttcggc
      361 ggagaagttt tcgcccagcg gcagccccag ggcattctcc gggccgacgg ggaagaggtt
      421 ccggtagatg acgtagccgg cagcaccggg cgttgcgtcc cacatcagcc gcacggcctg
      481 gttggtggcc ggctcggcga gtcgaagacc ttccgtgagc ggcagcaccg atgagccgtc
      541 ccagggctgg tagggcttca ccacggcggc acgccggtcc gtgcccggtg gctggctgat
      601 ctggtccacg tcctgggccg ccgggtccac ggtccacacg aacgggccgg cgatggtgaa
      661 ggccagcagc cccagcacga tgtagagcga gaacagcgcg cggcggttgg ccttcaggcg
      721 gatccaggcg tcctgccagt aggacaggga cggccgggag atggtctgga tccgccgctc
      781 ggcgtgaacg ggctcgaagt ctcgtgcggt gagggcagtc tcggccatgg gctactcgag
      841 cctcacgcgc gggtcgatga gcccgtagag caggtccacc acgatgacca tgaagaccag
      901 gaacgagccg tagaataccg tggtgcccat aatcaccgtg tagtcgagct gctgaacggc
      961 ctgcacgaag tagcggccga gaccgggaat ggcgaagacg agctccacca cgaagccccc
     1021 cgtggtgatg gcggcgatgg acgggcccag cacggtgatc acgggcagga tcgcgttgcg
     1081 cagctggtgc ctggtgaaga tgcgcgtggg cggcagcccc ttggacttgg ccgtgcggac
     1141 gtagtcggcg ttgacgattt ccagcatgga ggagcgcatg agccgcgtca ggtaagccat
     1201 ggtgccgagc ccgaggacca tggacggcac cagcatgtgc cacacgctgc cccagccggc
     1261 cacgggcagt atcgagacgc ccagcaggtc gttgaggttg accaggctca gctggctgat
     1321 ggcggcgatg acgaagctcg gcacggaaat gcccaggatc accaggaaca tgatgatcac
     1381 gtccggcagc cgattgcggt acagggccgt cagggcgccc cacagcacgc cgccgaggcc
     1441 ggcgaacacc accgcgagga tgcccagggt ggcggagacg gggaagtgct cacggatgat
     1501 gtcgttgacc tcgcggttct cctgagtgaa ggagatgccg aaatctcctt ttacgatgtt
     1561 gcccatgtag atcaggtact ggtcgaaaac aggcttgtcg aggccgtatt ttgcttccag
     1621 gttggcgcgg atctctgccg tgacggcttt ctcgtcggcc aaggggtcgc cgggcacgct
     1681 gtgcatggcg atgaaggtgg cggtagctat gaaccagacg gtgactatgc cctggagcag
     1741 acgcttgagc gtgtagttga gcatgtctta agcgtcagcc ttccacgatg taggcgttgg
     1801 tgaagtccgg gtccgggccc acggagcggc gcacgagccc gccgaggtcg gggttcacca
     1861 cgtaggtcgt gccgggctcg tagttgatca ggatcaccac gtcgtcgtgc aggatccgct
     1921 gtatctcgcc gaacgcgtcc atcctcgtct tgggatccag ggactcctcg gcgatggcta
     1981 cctggcgatc cagctcgggg ttgctgtagc ggccccggtt gttgaggttc caggaggcga
     2041 acaggtcgcc gaaggtgagc gggtcgtcgt agtccgggcc ccagccggct agcaccatgt
     2101 cgaaatcgcc cgaagtcatc ttggccaggc gctgcttgaa gatctgcttg tctatgcgga
     2161 tgtcgagacc gagggtctgc ttgaacacct gctggaagta ctcggactga atgttggaga
     2221 cgggattgtc gccgcttagc agcaccagcg gggggatctg ctccatcccc agctcccgct
     2281 tcgccgcctg gagatactct ctggccttga ccacgtcttt gatcgtcttc ggcgccgggt
     2341 actcctggcg gaagtaaccg ttcacgccgc gcagccagac ggggaacagc gattcgccgg
     2401 ggatgtagcc gggcagcttg gtcaccttgt agaccagctc cgccgggtcc acggcgtgct
     2461 gcatggcctt gcgcaggttc gtgttcgcgg tgacgcgccc gtcgcgaaag ttgaactcga
     2521 tgaagaagac cgagccgtcc atgaatcgct tgatgtgcca gcgctgctcc atggcgtcgt
     2581 tgagattctc cgccagcagc ccggcgtagg cgaccttgtt gtccttgaag agattcagcg
     2641 tcgcgttggc gtcggaagtg atgtacggaa agtcgagcac ctcgatgcgg atccgttcct
     2701 catcccagta cgtggggttc ttctccagcc tcacgtgggc gccgtgcacc cagcgcgtca
     2761 tgcggaacgg gccgttgtag agcagcgtgt gcgcatccgc gccgtagcga cccttggttg
     2821 cctcgtaaaa atccctgcgg atcgggtagt aagtcgggaa agccacgagc ttgtcgaagt
     2881 aggccaccgg ccgctcgaac gtgacctcga gggtgcggtc gtccacggcc cgcgcgccga
     2941 gctgcgaaac ggggagcttg gcctcggcga tggcccgccc gttcttcacc gggtagagga
     3001 tgaaggcgta ctgggacgcg ttggcggggt ccagggccag ctgccaggcg aacacgaagt
     3061 cgtgggcggt gacgggcgca ccgtcgctcc acaccgcgtc gctgcgcagg tgaaagatgg
     3121 cgccgttggc gtcgatctcc cagcgctcgg ccatacccgg caccagccgg ttgtgctcgt
     3181 cgtagcgcag caggccttcc atcacgtggc ccagcaccat gccgctgacc atgtcggtgg
     3241 ccagggagct gttcagctgg gggggttcct cgcgcaggaa gagcgtgacc gtgcgtgtct
     3301 ccggatccac ggcaacctcg gagccagcgc cggacccggt caggctggaa gcccagacca
     3361 gcgcgtacat cagcagcccg atgctgccga tgctgatggc ggcgaacagg agaagctgcc
     3421 gcccggcgga ccggttgaag gtgtcctcgc ttcctgcgac cgttgtcacc gcgctccgct
     3481 tcctactgaa cgacccgcag cagattaccc acaatgcgct cgagatgca
//
LOCUS       DBNHOX010000017         2498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1029869, whole genome shotgun sequence.
ACCESSION   DBNHOX010000017 DBNHOX010000000
VERSION     DBNHOX010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1029869"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            423..1409
                     /locus_tag="AC16UZ_00390"
     CDS             423..1409
                     /locus_tag="AC16UZ_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024852.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HIR6245162.1"
                     /translation="MSSATRGTLLLTGASGFVGRHAHGALGDAGWHVRCATRDLDRAH
                     RRWPERDWVSLDVADRASVVSALEGCEAALYLIHGMASHDGDFREAEVRQAEVFATAA
                     AVAGLDRIVYLGGVAAPSDASDHLRSREEVGEVLRAGSVPTVELRASMIVGHGSLSWL
                     IVRDLAARLPIMILPRWLESRTEPVAIDDVVTAVVRALDLEVSGSAWFDVPGPEILSG
                     REILERTAEALGVRRAFMIQVPFLSPGLSSHWVRFVTRAEWSVAREIVVGLKTDLIAR
                     DDRFWSLIDHEDRLSFDEAARRTIEMELAGPPIRGFWGLIERMRVRIARIIE"
     gene            1511..2059
                     /locus_tag="AC16UZ_00395"
     CDS             1511..2059
                     /locus_tag="AC16UZ_00395"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR6245163.1"
                     /translation="MYFGIWLAMGTTAAVLGVAAVTSEGRAVLGRGFHSRVWLIGFPA
                     GLLMAGGTALLFGPMTSAFPALTEDVERLYATFRGPGLAVTLLLMPLVVTCEEIVWRG
                     AVYEALRVRISWVPTVLVGTAAYSVALVPIGSPALVLTAIGAGLCWNLLRARTDSLTA
                     AISAHLVWDYAILVVFDVTALG"
BASE COUNT          362 a          765 c          896 g          475 t
ORIGIN      
        1 cgcggaggcg tgagtcgtgc acgcgtcctc ctggtggagg cgaaggcatt gggacgtcga
       61 cagcagtgac gtcccgcggc tcctccgggc cgcgctcgag gctcgcttcg gtaggctccc
      121 ggcgatcgtc ttcgagcgtg ctcagcgatg ggggtacgcc tcggcttcgg gccccgcccc
      181 ggtacccggt ggcgcgaaga ctcggaactg tgggctgcag gtgactggtg tgtcggtgga
      241 cgtgtagagg gcgcgctggt atccggcatc gagatcgcgg atcgggtgat cgcttcggcg
      301 tgacctgcga gccggcccgt gacattggcg agcgtgccct ccacattggc ccagccacgc
      361 aggccaggaa gaacgcggtc tcccgcgttg gacggaccct cccgaactcc gagccgttgg
      421 tgatgtcatc agccacacgt gggaccctac tgcttacggg cgccagcggc ttcgtgggac
      481 gacatgccca tggagcgctc ggggatgccg gctggcatgt acggtgcgcc actcgtgacc
      541 tggaccgggc ccatcggcgg tggccggagc gcgattgggt gagcctggac gtcgccgacc
      601 gggcgtcggt ggtgagtgcc ctcgagggct gcgaagcggc cctgtacctg atccacggca
      661 tggcctcgca cgacggagac ttccgagagg ccgaggttcg gcaggcggag gtgttcgcca
      721 cggcggccgc cgtggcggga ctcgatcgca tcgtatacct ggggggcgtg gcagcgccga
      781 gcgacgcgtc cgaccatctg cggagccgcg aggaagtcgg ggaggtgctt cgggccggct
      841 cggtccccac ggtagagctg agagccagta tgatcgtagg ccacggaagc ttgagctggc
      901 tcatcgtccg ggatctcgcc gcgcgacttc ccatcatgat cctgccgagg tggctcgagt
      961 cacgcacgga gcctgtcgcg atcgacgacg ttgtcaccgc cgtcgttcgc gctctggatc
     1021 tcgaggtttc gggcagcgcg tggttcgacg tgccgggacc cgagatcttg tccggtcgcg
     1081 agatcctgga gcggaccgcc gaggccctcg gggtacgacg ggccttcatg atccaggttc
     1141 cgttcctctc tccggggctt tcatcacact gggtgcgatt cgtcacgaga gctgagtggt
     1201 ccgtggcccg cgagatcgtc gtgggactca agacggattt gatcgccagg gacgatcgct
     1261 tctggtccct gatcgatcac gaagaccgac tgagcttcga cgaagccgcc cggcggacga
     1321 tcgaaatgga gcttgcaggt ccgccgattc gaggattctg gggcctgatc gagcgcatgc
     1381 gtgttcgaat cgcccggatc atcgaatgag cggtagagtg gccggggtcg atcccggccc
     1441 ggggctgacg cccgcagaat ggcggggcgt cgcgtactgt ctcatgtggg cctcaggtgc
     1501 ttggctttcg gtgtatttcg ggatctggct tgctatgggt acgacggccg cagttctcgg
     1561 cgtcgcagcc gtgacctctg aaggccgggc tgtcctgggt cgtgggtttc actcgcgtgt
     1621 gtggctgatc ggtttcccgg ccggtcttct gatggcgggc ggcacggccc tgctcttcgg
     1681 accgatgacg tccgcctttc cggccctcac ggaggatgtc gaacggctgt acgctacctt
     1741 ccgaggtccc gggttggccg tcacgctcct gttgatgcct ctggttgtga cctgtgagga
     1801 gatcgtttgg cgaggcgccg tctacgaggc tcttcgggtg aggatctcgt gggtaccgac
     1861 ggtactcgtt ggcacggctg cctactcggt ggcgctcgtg ccaatcggct cgccggcgct
     1921 cgtgcttact gcgatcgggg caggcctctg ttggaatctg ctacgtgcgc gcaccgacag
     1981 cctcacggct gcgatctcgg cgcatttggt ctgggactac gccatcctcg tggttttcga
     2041 cgtcaccgcg ttgggctgac gccagggtct accccgtgaa ggaagtgaag caagacaggg
     2101 gcttcgcgtt gaaggtccgg aaggaggagc tggctgcgta gctcggtcgt ctcgcgcgtc
     2161 ggtctcctct cccgctctca gccggtccgc tgagggcccc aatcgagctc gcctctacgt
     2221 gagtcgacct ggaccgagaa acgggggtgg agcagtcggg gcacttctgg acgtcgccat
     2281 gccaccggcg tcaccgggcg aggttctagc gtaactgccc ccggggcatc aagccggtcc
     2341 agaaaccacg gaggacgttc ggttcctgag acacagcggc ggggtcgatc caaaggcagg
     2401 ggagcgggga tcccgaagca cgagatctcc gccctcgagg aattgtcgca ggccgcacga
     2461 cggtcgcagc tactgagatc tcgcggaagc gcctgccg
//
LOCUS       DBNHOX010000018         3605 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_130011, whole genome shotgun sequence.
ACCESSION   DBNHOX010000018 DBNHOX010000000
VERSION     DBNHOX010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_130011"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1252)
                     /gene="nrfD"
                     /locus_tag="AC16UZ_00400"
     CDS             complement(23..1252)
                     /gene="nrfD"
                     /locus_tag="AC16UZ_00400"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR6245164.1"
                     /translation="MSGSSPTALYEQLSHQLEQVIGYIYPNEVEIQWSILIVVYPFLT
                     GIVAGAFILASLVKVFNVKEVQPTYRLALLTALAFLLIAPLPLQMHLGHPERALEIFL
                     TPQTTSAMAMFGFVYAWYLMGILLLEVWFEYRHQLIVIADGARGLTKLFYRVLSLFSR
                     DTSPEALAFDKRASRVITIVGIPSAFLLHGYVGFIFGSVKANPWWGSVLMPIVFLMSA
                     IVSGIAMVILLYMILSLARNEKLDMQCLDKMASFLFIAVIVDFSLEALDFIHRLYQSE
                     ESVKILGELISVKLFMNLVVLQVILGMFVPLALLIVARTGRFVDDMRKLLYFLSVILI
                     LMGIFSTRWNVVIGGQLFSKSFRGLMGYKLEITGIEGLLTAIVLLVAPFVILMILLRI
                     LPLRRLEDKPPPDAATA"
     gene            complement(1249..2010)
                     /locus_tag="AC16UZ_00405"
     CDS             complement(1249..2010)
                     /locus_tag="AC16UZ_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6245165.1"
                     /translation="MDKRDFLKDAILFIPAAGLLKTLAGSTEAEASTSEGSGQAGGEG
                     NGAWYGMGIEVEKCIGCNRCVEACKAENDVPSEPYYFRTWIERYTVHSNGETTVECIS
                     TNAEPQPEVDDRDILRTFFVPKLCNQCANPPCVQVCPVGATFQTADGVVLVDKTRCIG
                     CRYCIQACPYGARYLDPRSRTADKCTFCYHRITNDQLPACVEVCPTQARIFGDLHSRA
                     SRLVRFQRMNKISVLKPDLNTEPKVRYAQLDGEVR"
     gene            complement(2010..2741)
                     /locus_tag="AC16UZ_00410"
     CDS             complement(2010..2741)
                     /locus_tag="AC16UZ_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245166.1"
                     /translation="MKIPEQVTRLGIVLAALLGLILVLRFVVLPADMFSAKPHQVAAT
                     ERERAKQASYAGVSVCKQCHTDEFETKSAGYHRNVACETCHGPALGHAEAPKSAKPPA
                     PRDRKFCPVCHGYDPSRPTGFPQINQANHNPMKPCITCHDPHDPVPPETPQECSACHG
                     QIASTKALSSHALLACTTCHEAEEQHKIQPRSSRPSKPDSREFCGQCHSSEHGVSRAP
                     KVDMNQHGDTFLCWQCHYPHLPEGN"
     gene            complement(2992..>3605)
                     /gene="nrfD"
                     /locus_tag="AC16UZ_00415"
     CDS             complement(2992..>3605)
                     /gene="nrfD"
                     /locus_tag="AC16UZ_00415"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR6245167.1"
                     /translation="LGPRFIASAFASGPALIIMVLQAVRKFTDYPVSQSVIDLLALIM
                     TFALQISVFFVGVELFTDFYNEGAHAASIHYLFLGIGDFGALRPWIWSALTMNVTAMI
                     ILMIHPLRRNIVTLNVACVLAFIGIWIEKGMGLVIPGFIPTPLGEIFEYTPTGKELLI
                     TAGVWALGVLVFTLVTRPVIAIQVGRLRYEKVPGPAQVKDFIR"
BASE COUNT          684 a         1091 c         1192 g          638 t
ORIGIN      
        1 caccgccacc ccggcctgtg cttcaggcag tcgccgcgtc gggaggcggc ttgtcctcga
       61 gccggcgcag gggcaggatc cgcagcagga tcatcaggat cacgaacggg gcgaccagca
      121 ggacgattgc ggtaagcagg ccctcgatcc ccgtgatctc cagtttgtag cccatcaggc
      181 cgcggaagct cttcgagaag agctggccgc cgatcaccac gttccagcgc gtgctgaaga
      241 tccccatcag gatcaggatc accgacagga agtagagcaa cttccgcatg tcgtcgacga
      301 agcgtccggt cctcgcgacg ataagcaggg cgagcgggac gaacattccc aggatgacct
      361 gcagcacgac gagattcatg aacagcttga cggaaatcag ctcgccgagg atcttgaccg
      421 attcctcgct ctggtagagg cggtgtatga aatcgagcgc ctccagggag aagtcgacga
      481 tgacggcgat gaagaggaac gatgccatct tgtccaggca ctgcatgtcg agcttctcgt
      541 tacgcgcgag gctcaggatc atgtagagga ggatcaccat cgcgattccc gagacaatcg
      601 cggacatcag gaacacgatc ggcatcagga cgcttcccca ccatggattg gccttgacgg
      661 agccgaagat gaagcccacg tagccatgga gcaggaacgc ggacgggata ccgacgatgg
      721 tgatgacccg gcttgcccgc ttgtcgaagg ccagcgcctc cgggctggta tcgcgcgaga
      781 acagggacag cacgcgataa aagagcttgg tcaacccgcg ggccccgtca gctatgacga
      841 tcagctggtg ccggtactcg aaccagacct cgagcaagag gatacccatc aggtaccagg
      901 cgtagacaaa gccgaacatc gccattgccg aggtcgtttg cggcgtcagg aagatctcca
      961 gggcgcgctc gggatgtccc aggtgcatct gcagcggcag cggcgcgatc agcaggaacg
     1021 ccagggccgt gagcagcgcc agccggtagg tgggctgcac ctccttgacg ttgaagacct
     1081 tgaccagcga ggcgaggatg aaggccccgg ccacgatgcc cgtcaggaag ggatagacga
     1141 cgatcagtat cgaccactgg atctcgacct cgttggggta gatgtagccg atcacctgct
     1201 cgagctggtg ggacagttgc tcatacagtg ccgtgggtga cgagccgctc atcgaacttc
     1261 tccgtcaagc tgggcatagc gaaccttggg ttcggtattc aggtcgggtt tgaggacgct
     1321 gatcttgttc atcctctgga agcggacgag ccggctcgca cgactgtgca ggtcaccgaa
     1381 aatgcgtgcc tgcgtcgggc aaacctccac gcaggccggg agctggtcgt tggttatccg
     1441 gtgatagcag aacgtgcact tgtccgccgt gcggctgcgc ggatccaggt agcgtgcgcc
     1501 gtaggggcag gcctggatgc agtagcggca gccgatgcag cgggtcttgt cgaccaggac
     1561 cacgccgtca gccgtctgga acgtcgcacc gaccgggcag acctgcacgc acggcgggtt
     1621 ggcgcactgg ttgcatagct tgggaacgaa gaacgtccgc aggatgtccc ggtcgtcgac
     1681 ctcgggctgc ggctccgcgt tggtgctgat gcactcgacc gtggtctcgc cgttgctgtg
     1741 gacggtgtag cgctcgatcc aggtcctgaa gtaatacggt tcgctgggca cgtcgttctc
     1801 tgccttgcag gcctcgacgc aacgattgca gccgatgcac ttctcgacct cgatgcccat
     1861 gccgtaccag gcgccattgc cctcgccgcc cgcctggccc gaaccttccg aggtcgatgc
     1921 ctcggcctcg gtggaccctg cgagcgtctt cagcaagccc gccgcgggga tgaagaggat
     1981 ggcgtccttg aggaaatcac gcttgtccat cagttcccct ccggcaggtg cgggtaatga
     2041 cattgccagc acaggaaggt gtcgccgtgc tggttcatgt ccaccttggg tgcacgcgac
     2101 accccgtgtt cgctgctatg gcactggcca cagaactcgc ggctgtcggg cttgctgggc
     2161 ctcgacgagc ggggctggat cttgtgctgc tcctcggctt cgtggcaggt ggtgcaggcc
     2221 agcagcgcat ggctcgacag ggccttggtg ctcgcaatct ggccatggca cgcggagcat
     2281 tcctgcggcg tttccggcgg caccggatcg tgcgggtcat ggcaggtgat gcacggcttc
     2341 atcgggttgt ggttagcctg gttgatctgc gggaagccgg tgggccggga cgggtcgtag
     2401 ccgtggcaga ccgggcagaa cttccggtcc cgcggtgccg gcggcttcgc agacttcggg
     2461 gcttccgcat ggccaagcgc ggggccgtgg caggtttcgc aggccacatt acggtgatag
     2521 ccggcgctct tggtctcgaa ctcgtcggtg tggcactgct tgcagacact gacgccggcg
     2581 tagctcgcct gcttggcgcg ctcccgctcg gtcgcggcca cctggtgagg cttggccgag
     2641 aacatgtctg caggaaggac gacaaaccgg aggacgagga tcagccccag cagcgcggcc
     2701 agcacgatgc cgagacgtgt aacttgttca ggaattttca ccgcctaccc atccgctcag
     2761 tacgaatcga gatgaatcgc ctaggcacga tcgtcggtac ggttccggaa tcagccaacg
     2821 cttggcccac cttgcccgcg gcgcccttcc ttgggcgtcg tcattctgga cgacggtgga
     2881 gacgaacccc ctggaacgtg cctcaaagct tggcgccata ttagggggtc ggacgggcgt
     2941 tgaatctagg taatttcagc actggaatag cggcaaccga cccgcccggc gtcagcggat
     3001 gaagtccttg acctgggccg ggccggggac cttttcgtag cgcaaccggc cgacctgtat
     3061 ggcgatcacc ggccgtgtca cgagggtaaa gaccagcacg cccagggccc acacgcctgc
     3121 cgtgatcagc agttccttgc ccgtcggcgt gtactcgaag atctcgccca gcggcgtagg
     3181 gatgaacccc gggatgacca ggcccatgcc cttctcgatc cagatgccga tgaatgccag
     3241 gacgcaggcg acgttgagcg tcacgatgtt ccggcgcagc gggtggatca tgaggatgat
     3301 catcgcggtg acgttcatgg tgagcgccga ccagatccag ggccgcagcg ccccgaagtc
     3361 gcctatgccc aggaacaggt agtggatcga cgcggcgtgc gcgccttcgt tgtagaagtc
     3421 ggtgaacagc tcgacgccga cgaagaacac gctgatctgc agcgcgaagg tcatgatcag
     3481 cgccagcagg tcgatcacgc tctggctcac ggggtagtcg gtgaacttcc tgaccgcctg
     3541 caacaccatg atgatcaggg ccggccccga ggcgaaagcc gaggcgatga accgcgggcc
     3601 cagca
//
LOCUS       DBNHOX010000019         2767 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_901590, whole genome shotgun sequence.
ACCESSION   DBNHOX010000019 DBNHOX010000000
VERSION     DBNHOX010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_901590"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="AC16UZ_00420"
     CDS             <1..1171
                     /locus_tag="AC16UZ_00420"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HIR6245168.1"
                     /translation="FDDPLYVMYSSGTTGKPKCIVHGIGGTLIQHLKEHQLHTDLRRD
                     DRLFYFTTCGWMMWNWLVSGLATGATLVLYDGSPFHPGPEALLDMAEQEDVTVFGVSA
                     RYIAALQKTSLKPMETHDLSPLRAILSTGSPLTHEGFRYVYEHVKRDVLLASISGGTD
                     IISCFVLGNPTLPVWEGEIQCRGLGMAVEVVDDGGRPVTGEKGELVCTRPFPSCPLGF
                     WNDTDGAKFRAAYFQRFPGIWAHGDFAEVTEHDGFVIHGRSDAVLNPGGVRIGTAEIY
                     RQVEHVDAVLESLCVGQEWEGDTRVVLFVVMRPGRELDEALAQQIRQRIRQHASPRHV
                     PARIVQVADIPRTLSGKIVELAVRETIHGRPVQNTSALANPEALDLYSNLPELES"
     gene            complement(1225..2073)
                     /locus_tag="AC16UZ_00425"
                     /pseudo
     CDS             complement(1225..2073)
                     /locus_tag="AC16UZ_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
     gene            complement(2119..2649)
                     /locus_tag="AC16UZ_00430"
     CDS             complement(2119..2649)
                     /locus_tag="AC16UZ_00430"
                     /inference="COORDINATES: protein motif:HMM:NF049802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2371..2373),aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HIR6245169.1"
                     /translation="MTIIMDPTDERVPVARQLTPRSGAVAGSVALLDISKPRGSVLLD
                     RLEELLKERLPGIVVNRYAKPTFTKPAPETLRREIFASNDFVVEGLADUGSCTTCSLH
                     DTVWFEIQGKPAVSIASSEFGDAADTQARALGMEDACCVFVPHPIQDATDDEMRAKAD
                     GVVEAVIEALTVGGSG"
BASE COUNT          493 a          935 c          841 g          498 t
ORIGIN      
        1 ttttgacgat cctctgtacg tcatgtactc gtccggaacc accggaaagc ccaagtgcat
       61 cgtccacggg atcggcggca ccctcatcca gcatctcaag gagcaccagc tccacacgga
      121 tctccgccgg gatgatcggc tgttctactt caccacctgc ggctggatga tgtggaactg
      181 gctggtcagc gggctcgcca ccggtgccac gctggtactc tacgacggct cgccctttca
      241 tccgggtccc gaagccctgc tggacatggc cgagcaggaa gacgtcacgg tgttcggcgt
      301 cagcgcccgc tacatcgccg cccttcagaa gacttcgctc aagcccatgg agactcacga
      361 tctttcgccg ctgcgagcca ttctgtcgac cggctcgcca ctgactcacg aaggcttccg
      421 ctacgtctac gagcacgtca agcgggacgt gctgctcgct tcgatttccg gcggcaccga
      481 catcatttcc tgcttcgttc tgggcaaccc cacgctgccg gtttgggaag gcgagattca
      541 atgccggggg ctcggcatgg cggtcgaggt ggtggacgac ggcggccgac cggtcaccgg
      601 ggaaaagggc gagctcgtct gcacccggcc gtttccctcc tgccccctcg gcttctggaa
      661 cgacacggac ggcgcgaagt tccgtgccgc ttacttccag cgtttccccg gcatctgggc
      721 tcacggcgat ttcgccgagg tcaccgagca cgacggcttc gtcattcacg ggcgcagcga
      781 cgccgtgctg aatccgggag gcgtcaggat cggcacggcc gagatctacc gtcaggtaga
      841 gcacgtcgac gcggtgctcg aaagcctgtg cgtcggccag gagtgggagg gagacacccg
      901 cgtcgtgctc ttcgtcgtga tgcggcccgg ccgcgagctg gacgaagcgc tggctcagca
      961 gatacgccag cgcattcgtc agcatgcatc gccccgtcac gttcccgcgc gcatcgtgca
     1021 ggtggccgat atcccccgaa cgctgagcgg caagatcgta gagctggcgg ttcgagagac
     1081 catccacggg cgccccgtgc agaacacgtc ggctctcgcg aacccggagg ccctagatct
     1141 ctatagcaac ctgccagaac tcgaaagctg aggccgccca cgcccgccag cccgatcttt
     1201 gcgccccctc ttggttccgt cccctcaagg gcccagcggc tctccttcga tggtgatccg
     1261 atgcagcagg cgtcgcttgc cgtggtagtc gttgagggcg taatgccagg ttgcacggtt
     1321 atcccagagg gccaacgaac caggctgcca acggaaccgg tacgtgaact cagcacgcga
     1381 cgcatgctgg tacaggtaga gcagcagcgg cttcgactcc tcctcggtcc agtcttcgaa
     1441 gcgaacggtg aatcccggat tgacgtaaag ggcccgacgg cctgacaggg gatgcgtgat
     1501 gatgaccggg tgcacggcat cctgaaccgc gagctccgga ttgccgatgc gaccgctcgg
     1561 atccgccggg tgcgcgctgc caaacacgtg acggctggag tgaacggccc gcagccggcc
     1621 aagcatctcc ttgagcccgt ccgacagcgc gtcgtaggcc gcgtacatgc tggcaaactg
     1681 ggtgtcaccg ccctgttccg gaacgtcgat ggcatacagc agcgacccca tggccggcac
     1741 gtcgtcgtat gagtgatccg tgtgccagcc accgccgatg ttgacgcgct gatccggctc
     1801 cttgcgaacc tcggcgatca tcgggaaccc gaccaccggc cggaagaatc ggttgatgtt
     1861 tatagtcccc cagcgttagg cgaagcgaac ctggtcatcg ggcgcaagct tctggtcgcg
     1921 aaagaacagc accccgtgtt ccgcaaacgc gttgcggatc gtctcgaagt gctcgtcggg
     1981 cagtcgctgg cagaggtcca cgccggtgac ctcggcgcca agcgcgccgc caacgggctt
     2041 tatctgcaat ccttcacgcc cttgctcgct catcgcgcgt tcctgccccc ctttcgcccg
     2101 gggcgattcg aatctcaact aacccgagcc gcccaccgtc agggcctcga tgacggcctc
     2161 cacgacgccg tcggcctttg cgcgcatctc gtcgtcggtc gcgtcctgga tcgggtgcgg
     2221 cacgaagacg cagcaggcgt cctccatccc gagcgcgcgc gcctgggtgt ccgctgcgtc
     2281 accgaactcg ctggacgcaa tggaaaccgc gggctttccc tgaatctcga accataccgt
     2341 gtcatgcaaa ctgcacgtgg tacaggatcc tcagtcggca agcccttcga cgacgaaatc
     2401 gttgctcgcg aatatctcac ggcgcagcgt ttcaggcgcc ggcttggtga acgtcggctt
     2461 cgcgtagcgg ttgaccacga tgcccggcag acgctctttc aacagctcct ccagccgatc
     2521 gagcagcacg ctgccacgcg gcttggagat gtccagcagc gccacgctgc ccgcaaccgc
     2581 gccgcttctc ggcgtcagct gccgggcgac gggcacgcgc tcgtcggtgg gatccatgat
     2641 gatggtcatt caacctgcct cctctatttt ccgggatacg gaaatggaac caatctctcc
     2701 agtaacccag ccatgaaagg ccccggagaa cttaccggca tcagagccgg cgacgacgag
     2761 atgaatg
//
LOCUS       DBNHOX010000020         2072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_580194, whole genome shotgun sequence.
ACCESSION   DBNHOX010000020 DBNHOX010000000
VERSION     DBNHOX010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_580194"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="AC16UZ_00435"
     CDS             <1..395
                     /locus_tag="AC16UZ_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245170.1"
                     /translation="APYVMLGFNVCAADTEEEAVYLRSSSLQAILQLRQGAPGRLPPP
                     VHDFDATLTPEQRYLIDRFSLCSAVGTRETVERSINDFLARTGADELIIVGQIFDHQA
                     RLRSFEIVAELIGDLQPGDSTRVAGQHG"
     gene            388..1545
                     /locus_tag="AC16UZ_00440"
     CDS             388..1545
                     /locus_tag="AC16UZ_00440"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6245171.1"
                     /translation="MDDELDLMGPEEMADPAVSARRLLEGPRCRLYHGFDPPFYVVSR
                     YDDVSAIVRDPGRFLSGHGQGPNFVAPMGVVSDPPQHTFFRELVQPDFLPRSIALLRP
                     RLEAIADELLDAVAEETVWDVHDRLSFPLPVTIICEIFGIPTDDIEQFKIWSDASVAA
                     LSSQNPQQYDAELARMQAYVLERLRGTRRRPGDGSLISRIARARRDGVPVTDEEAVRL
                     VLQLFVAGNETTTSLITNFVWRMLAGDGLWADFCAGRVDVDRAINESLRFDPPLLGLF
                     RTTAVDVDVGGTVIPAHSKVLTHYGAANRDPAAFRDPDVFDVGRTGRKVLSFGIGIHV
                     CLGRELALLEARVALQGLARRFPRLELVDDGERIGPFLFWGRRHLPVRNCA"
     gene            1588..>2072
                     /locus_tag="AC16UZ_00445"
     CDS             1588..>2072
                     /locus_tag="AC16UZ_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR6245172.1"
                     /translation="MCQSHDVSNETENADPRSGYRLAEGWPHYPADMQFEMGSGVAVD
                     DAGVIYLFTRDIEHWAAHPLAMKDKMGKSSISMFDRAGNYLGKWGPSDERGFALGAHT
                     LYIEKDGMFWTTDRDGHTVRKYHPDGTLLLTLGEFGQWGAGTDHFNGPTAVLVQENGN
                     IV"
BASE COUNT          334 a          704 c          663 g          371 t
ORIGIN      
        1 atgctcccta cgtcatgctc ggtttcaacg tctgcgctgc ggacacggag gaggaagccg
       61 tttacctgcg cagctcgtcc ctgcaggcaa tcctccagct gcgccagggt gcgccggggc
      121 ggctgccgcc gccggtgcac gacttcgacg ccaccctcac gccggagcag cgttacctca
      181 tcgatcggtt ttccctgtgc tcggcggtgg gcacacggga gacggtagag cgttccatca
      241 acgacttcct cgcccggacg ggtgccgacg agctcatcat cgtcggccag atcttcgatc
      301 accaggcccg gctgcggtcg ttcgagatcg ttgcggagct gatcggagac ttacagcccg
      361 gggactccac gcgggttgcc gggcagcatg gatgacgagc tcgacctgat ggggcccgag
      421 gagatggccg acccggccgt ctccgcccgg cgactgctcg aaggcccgcg ctgccggctc
      481 taccacgggt tcgacccgcc gttctacgtc gtctcccgct acgacgacgt gagcgccatt
      541 gtgagggatc cagggcgttt tctgagcggc cacggtcagg gtcccaactt cgtcgcgccc
      601 atgggcgtgg tgtccgaccc gcctcagcac acctttttcc gcgagctggt ccagcctgat
      661 ttcctgccaa gatccattgc cctgctacga cctcgcctgg aagccatcgc cgacgagctg
      721 ctcgacgcgg tcgcggaaga gacggtctgg gacgtccacg accggctttc ctttccgctg
      781 ccggtgacca tcatctgcga gatattcggc atacccacgg acgacatcga gcagttcaag
      841 atctggtccg acgcctccgt cgccgctctg tcctctcaga acccgcagca gtacgacgcg
      901 gagcttgcgc gcatgcaggc ctacgtgctg gaacggctgc gcgggacacg gcgccgtcct
      961 ggcgacggca gcctgatctc gcgcatcgcc cgtgcccgcc gagacggcgt gccggtgacg
     1021 gacgaggaag ccgttcgcct ggtgctgcag ctcttcgtcg cgggaaacga gaccacgacc
     1081 tcgctcatca ccaatttcgt ctggcgcatg ctggcaggcg acgggctttg ggccgatttc
     1141 tgcgcgggcc gcgtcgacgt ggaccgggcg atcaacgaga gcctcagatt cgatccgccc
     1201 cttctcggac tcttccgcac cacggccgtg gacgtcgatg tcgggggaac cgtgataccc
     1261 gctcactcca aggtgctcac ccactacggc gctgccaatc gggacccggc ggcgttccgg
     1321 gatccggacg tattcgacgt gggccggact ggccggaagg ttctttcctt tgggatcggc
     1381 atccacgtct gcctgggccg cgagctggct ttgctcgagg ctcgggtggc cctgcaaggt
     1441 ctcgcgcggc ggtttccgcg cctcgagctg gtggacgacg gcgagcggat cggcccgttt
     1501 ctcttctggg ggcgcaggca cctgccggtg cggaattgcg cctgacggcc atttcctaag
     1561 cgcctcggcc tcgcctacaa tgaagccatg tgccagtccc acgacgtatc caacgagacc
     1621 gaaaacgccg atccccgcag cggctaccgc ctggcggaag gttggcccca ttacccggcc
     1681 gacatgcagt tcgagatggg ctccggggtg gcggtggacg acgccggcgt gatctatctg
     1741 ttcacccggg acatcgagca ctgggccgct catcccctgg ccatgaagga caagatgggc
     1801 aagagcagca tctccatgtt cgatcgggcc ggaaactacc tcggcaagtg gggcccctcg
     1861 gacgagcgcg gcttcgcgct tggcgcccat acgctctaca tcgagaagga cggcatgttc
     1921 tggaccacgg accgtgacgg ccacacggtg cgcaaatatc atcccgatgg aaccctgctg
     1981 ctgaccctcg gcgagttcgg ccagtggggt gcggggaccg accatttcaa cggccccaca
     2041 gccgtgctcg tgcaggaaaa cggcaacatc gt
//
LOCUS       DBNHOX010000021         2009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1094379, whole genome shotgun sequence.
ACCESSION   DBNHOX010000021 DBNHOX010000000
VERSION     DBNHOX010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1094379"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..385
                     /gene="tusB"
                     /locus_tag="AC16UZ_00450"
     CDS             122..385
                     /gene="tusB"
                     /locus_tag="AC16UZ_00450"
                     /inference="COORDINATES: protein motif:HMM:NF016003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HIR6245173.1"
                     /translation="MATLHLVNRPSALASCLETARDEDTILLLEDGVYAAAASDVDRP
                     LHAIDVDVMARGLSRRLRENVTLASYDEFVRLVEDHQPVVSWR"
     gene            388..708
                     /locus_tag="AC16UZ_00455"
     CDS             388..708
                     /locus_tag="AC16UZ_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019826557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HIR6245174.1"
                     /translation="MQEASIETDKEGYLVDLADWSEAVAEQLAANEGISLTDDHWALI
                     QIIRAFYDQFEVSPAMRVLVKHTREQLGEEKGNSIYLLKLFPGSPAKVLAKISGLPRP
                     TNCL"
     gene            complement(729..>2009)
                     /gene="cysG"
                     /locus_tag="AC16UZ_00460"
     CDS             complement(729..>2009)
                     /gene="cysG"
                     /locus_tag="AC16UZ_00460"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF007922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HIR6245175.1"
                     /translation="LLIRAGAAIFIVSPELSEATRALVDEHGLDVVPRRFRPSDVEGH
                     YLVVAATNDPDVNRIVYDMCVSRRVLVNTVDQPELCSAIFPSIVDRSPVVVAVSTGGS
                     SPTLARVVRGWLEARLPARLGELAGFVKERRERVKSVLGSDSERQAFWEALIQGPVAE
                     QVLTGRDEEAAAAFDELLPAAAETARRGSVALVGAGPGDPELLTLKGLRLLQAADVIL
                     YDNLANQRLLDYARRDAEKIYVGKKRKFPGIRQEGINALLVEHALAGRHVVRLKGGDP
                     FIFGRGGEETETLAEHDIPCIVVPGITAALGSASYAGIPLTHRDVSQSVRFVTGHRVY
                     DHVNLDWPELAKPGQTLVIYMGLTWLEEILEKLVGHGMNPATPAALVERATLPEQRVV
                     EGTVADLAEKVRSSDVKGPTTVIVGEVVRYRSGR"
BASE COUNT          362 a          688 c          638 g          321 t
ORIGIN      
        1 gcgagagatc gctggcggaa cgcaacttat cgccggccga tctcgtgctg cccgtcaagg
       61 cgctggcgcc agccgagcag gccgagctgc tgacgaatca ggacgcggtg ctgaacgact
      121 gatggccacg ctgcacctcg tcaatcgtcc ctcggcactg gcgagttgcc tggagaccgc
      181 ccgggatgag gacaccatcc tcctgctcga ggacggcgtg tatgccgctg cagcgagcga
      241 cgtcgatcgc cccctgcacg ccatcgacgt agacgtgatg gcccgaggcc tttccagacg
      301 cctccgagag aacgtgacgc ttgcgagcta cgacgagttc gtccggctcg tcgaggacca
      361 ccaacccgtc gtgtcctggc gctagccatg caggaggcct caatagagac ggataaggaa
      421 ggctatctcg tcgacctggc tgactggtcc gaagccgtgg ccgagcagct ggcggccaat
      481 gagggcatct cgttgacgga cgatcactgg gcgctgatcc aaatcatccg cgcgttctac
      541 gaccagttcg aggtttcccc ggccatgcgg gtgctggtaa agcacacccg cgaacagctg
      601 ggcgaagaga aaggtaacag catctacctg ctcaagcttt ttcccggcag cccggcaaaa
      661 gttctagcga agatctccgg gctgccgcgc ccgaccaact gcctttgagc gccgcggatc
      721 agcgccgcct accggcctga tcggtaccga acgacctcgc cgacgatgac cgtcgtcgga
      781 cccttcacgt cactggaccg aaccttctcc gccaggtcgg caaccgtgcc ctccaccacc
      841 ctctgctcgg gcagcgtggc ccgctccacc agagcagcgg gggtggcggg attcatgccg
      901 tgtcccacga gcttctccaa aatctcctcc agccaggtca aacccatgta gatcacgagg
      961 gtctggccgg gcttggcgag ctccggccaa tccagattga cgtgatcgta gacgcgatgg
     1021 ccggtgacga aacggaccga ctgggagacg tcgcgatggg tcagcggaat gcccgcgtag
     1081 ctcgcgcttc ccagcgctgc cgtgatgccg ggcaccacga tgcaggggat atcatgctcg
     1141 gcaagggtct ccgtctcctc tccccctcgc ccgaagatga acggatcccc gcccttgagg
     1201 cgaaccacat ggcggccggc cagggcgtgc tcgacgagca gcgcgttgat gccctcctgc
     1261 cggatgccgg gaaacttgcg tttcttgccg acgtagatct tctccgcgtc acgccgggcg
     1321 tagtcgagca ggcgctggtt ggccaggttg tcgtagagaa tgacgtcggc cgcctgcagc
     1381 aggcgcaggc cctttaaggt caggagctcc ggatcgccgg gacccgcccc caccagagcg
     1441 accgatcctc ggcgagcggt ttcggcagcc gccggcagga gctcgtcgaa cgccgcggca
     1501 gcctcctcgt cgcggccggt cagcacctgc tcggccacag gaccctgaat cagtgcctcc
     1561 cagaatgcct ggcgctcgga atcgctgccg agcacgctct tgacccgctc gcgccgttct
     1621 ttcacgaagc cggccagctc gccaagccgg gccggcaggc gggcttcgag ccagccgcgc
     1681 acgacgcggg ccagcgtggg ggaactgccg ccggtggaaa cggctaccac tacgggcgaa
     1741 cggtccacga tgctcggaaa gatcgcgctg cacagctccg gctgatcgac cgtgttcacc
     1801 agcactcgcc gcgagacgca catgtcgtag acgatgcggt tgacgtccgg atcgttggtg
     1861 gccgcgacca ccagatagtg cccctccacg tcgctaggcc tgaaccggcg cggaacgacg
     1921 tccagaccgt gctcgtccac cagagcacgg gtcgcctcgc tcaactccgg cgagacgatg
     1981 aagatggctg cgccggcccg gatgagcag
//
LOCUS       DBNHOX010000022         3043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_194715, whole genome shotgun sequence.
ACCESSION   DBNHOX010000022 DBNHOX010000000
VERSION     DBNHOX010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_194715"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /locus_tag="AC16UZ_00465"
     CDS             <1..1518
                     /locus_tag="AC16UZ_00465"
                     /inference="COORDINATES: protein motif:HMM:NF036578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HIR6245176.1"
                     /translation="SLGPTDDFGDRAGDGVDWYTASGVPGGETFEPTFSVHGFRYVEV
                     CGDLADDEPGVVAVAVNTDLDATATFESDHPLLDRLHANIVANQRGCALDVPNAGFSL
                     ERRIPLTGEARAFLGTAAMNLDVAAFLAKWLTDLADAQLPDGGFPSVAPVPPLVPALQ
                     QDGGPGWSDAFAACAWILYRHFGDRRVLERNYPALRTYVLGQAAAWPDCLARPGSHPL
                     GPADRGAVASLKATALFLQAVRLASRIAGVLGNLSDFEQFDGLAQSIRSAFRNRFVTH
                     DGLVVGDCVTSYTLALHLGLLERGERRAAFEELVAKLEDGEPPAVEPLAAPYLLGVLT
                     RGGRVDLAYLVLLGSRPGSWLYPVLHGASSLWDGETESSRLARGSVGEWLYTGLAGLD
                     LDDDLSETNNAFRRIRIQPHPPLRDGIELAGNVPPMRRVKASLDTVHGRYESAWEILE
                     DGFVLRVQVPGNCSALVVMPDGTEHEVVAGRHEFKQDFLGETDEIPILREIAEAS"
     gene            complement(1557..2588)
                     /gene="mnmH"
                     /locus_tag="AC16UZ_00470"
     CDS             complement(1557..2588)
                     /gene="mnmH"
                     /locus_tag="AC16UZ_00470"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HIR6245177.1"
                     /translation="MNGTDPETLLAEGARFIDVRAPSEFTRGAVPGAINLPILDDDER
                     HEVGSTYNNEGHNAAVALGYRLVSGAVRQARVAAWAEALDADPAAWIYCWRGGQRSQI
                     AQEWLHEAGYEVPRVEGGFKALRQCCLEVLARAPHALPWLVLAGRTGSGKTVLIRERP
                     DSIDLEGQANHRGSAFGGRSCTQPSPISFENALAVAFLDLDSQRLIVEDESRTIGRLA
                     LPAAWHERMQTAPVVVVEADLSERVENIRREYVDEPLAEGVTPGDLQARYQGALERIS
                     RRLGGDRCRAVARALAEGFASGHHHAWIEQLLLWYYDPMYDYQLSKKQDRIVMQGDRD
                     AVREFLATP"
     gene            complement(2585..>3043)
                     /locus_tag="AC16UZ_00475"
     CDS             complement(2585..>3043)
                     /locus_tag="AC16UZ_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HIR6245178.1"
                     /translation="GAINLPILDDDAEAMILLGNLHARGVGVKQSLRRAVGWYKDAVD
                     ASGGDANIVNEVAWTLTVSDLEGLKRTRYALNIMEDLMTRDDEARRKPEYLDTWAATY
                     AANGDFIRAVAVQEEAVEAAEDDQYEGVREIIKTHLDAFKSGKTITETAP"
BASE COUNT          429 a          988 c         1059 g          567 t
ORIGIN      
        1 agcctgggcc cgacggatga cttcggcgac cgggcaggag acggcgtcga ttggtacacg
       61 gccagcggcg tgcccggcgg agagaccttc gaaccgactt tcagcgtaca cggcttccgg
      121 tatgtagagg tttgcggcga tctggcagac gacgagccgg gagtggttgc ggtagccgtc
      181 aataccgacc tggacgccac ggccacgttc gagtcggacc atcccctgct ggatcgcctg
      241 cacgccaaca tcgttgccaa ccagcgcggc tgcgccctgg acgtacccaa tgcgggcttc
      301 tccttggaac gcaggattcc gctgacgggg gaggcgcgtg cctttctcgg caccgcggcc
      361 atgaatctcg acgtggcggc ctttctggcg aagtggctga cggatctggc agatgcgcag
      421 ctgccggatg gggggttccc cagcgtcgct cctgtgcctc cgctggtgcc cgcgctgcag
      481 caggacgggg gcccgggctg gtcggacgcg tttgcggcat gtgcctggat tctctaccgg
      541 catttcggag atcggcgggt tctcgaacgc aactatccgg cgctgcggac ctatgtgctt
      601 gggcaggcgg cggcctggcc ggactgtctg gccaggccgg gcagccatcc gctcgggcct
      661 gccgaccgag gtgccgttgc ctcgctgaag gcgacggctt tgtttcttca ggcggttcgg
      721 cttgcctcgc gcattgccgg ggtgctgggg aatctgagcg acttcgagca gttcgacggc
      781 ttagcgcaga gtatccgttc cgccttcagg aaccgcttcg tgacccacga cggcctggtg
      841 gtcggcgact gcgtcaccag ctataccctt gctctgcatc tagggcttct ggaaaggggc
      901 gagcggcgcg cggcgttcga ggagctggtg gcgaagctgg aagacggcga gccgcctgca
      961 gtggaaccgc tggccgcacc ctatctcctt ggggtgctga cgcggggcgg ccgcgtcgat
     1021 ctggcctatc tggtgctgct cggctcgcgc ccgggcagct ggctgtatcc cgtgctgcac
     1081 ggtgcaagct cgctgtggga tggggaaacg gagtcgagcc gccttgctcg gggctcggta
     1141 ggcgagtggt tgtacacggg gcttgccggg ctcgatctgg acgatgatct atcggagacc
     1201 aacaacgcct tccggcgcat ccggatacaa cctcacccgc cgttacgtga cgggatagag
     1261 ctggccggca acgtcccccc gatgcgtcgg gtcaaggcgt cgctggatac cgtgcacggg
     1321 cgctacgagt cggcctggga gatcctcgag gacgggttcg tgctccgcgt ccaagtcccg
     1381 ggcaattgct cggcgctggt cgtcatgccg gacgggaccg agcacgaggt ggtggcggga
     1441 cgtcacgagt tcaagcagga tttccttggc gagactgacg agatccccat tctccgggag
     1501 attgccgagg cgtcttgatt ccctgcggcg gggttgggcc gccgtggggc agccggtcag
     1561 ggtgtggcga gaaactcccg gaccgcgtcg cggtcgccct gcatgacgat gcggtcctgc
     1621 ttcttgctca gctggtagtc gtacatgggg tcgtagtacc acagcagcag ctgctcgatc
     1681 caggcgtggt ggtggccgga ggcgaagccc tccgcgaggg cgcgcgccac ggcccgacag
     1741 cggtcgccgc cgaggcggcg gctgatgcgc tcaagcgctc cctggtagcg ggcctgcagg
     1801 tcgccgggcg tcacgccctc ggcaagcggt tcgtcaacgt actcgcggcg gatgttctcc
     1861 acccgctcgg acaggtcggc ttccaccacc accacgggcg cggtctgcat gcgctcatgc
     1921 caagcggctg gcagggcgag gcggccgatg gtccggcttt cgtcctccac gatcaatcgc
     1981 tgggagtcga gatcgaggaa ggccaccgcc agagcgttct cgaagctgat cggggatggt
     2041 tgcgtgcacg atctgccgcc gaacgcggag ccgcgatggt tggcttggcc ttccagatcg
     2101 atgctgtccg gccgctcacg aatcagcacg gttttgcccg agccggtccg cccggcgagg
     2161 accagccagg gcagcgcgtg cggcgccctg gccagcacct ccaggcagca ttgccgcaga
     2221 gctttgaagc cgccttcgac ccgggggact tcgtagcccg cttcgtgcag ccactcctgt
     2281 gcgatctggg agcgctggcc cccacgccag cagtagatcc acgccgcggg gtccgcgtct
     2341 agggcctcag cccaggcggc tacgcgggct tgcctcacgg cgccgctcac caggcggtac
     2401 cccagggcga cggcagcgtt gtgaccctcg ttgttgtagg tggagccgac ttcgtggcgc
     2461 tcgtcgtcat ccaggatcgg caggttgatg gcgccgggta cggcgccccg ggtgaactcg
     2521 gagggcgccc tgacgtcgat gaagcgcgcg ccttcagcca gcagcgtttc cggatcggtt
     2581 ccgttcacgg cgcggtttcg gtgatcgtct ttccgctctt gaaggcatcc agatgggtct
     2641 tgatgatctc ccgcacgccc tcgtattggt cgtcctctgc cgcctcgacg gcttcctcct
     2701 gaaccgccac ggcgcggatg aaatcgccgt tggccgcata ggttgccgcc caggtgtcca
     2761 ggtactccgg cttgcggcgc gcctcgtcgt cgcgggtcat gagatcttcc atgatgttga
     2821 gcgcgtagcg tgttcgcttc aggccctcca ggtcgctgac cgtcagcgtc catgccacct
     2881 cgttcacgat gttcgcatcg cccccggacg cgtccacggc gtccttgtac catccgaccg
     2941 cgcgccgcag gctctgtttt accccgacgc cgcgtgcgtg gaggttgccc agcaggatca
     3001 tcgcctccgc gtcgtcatcc aggatcggca ggttgatggc gcc
//
LOCUS       DBNHOX010000023         4221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1223013, whole genome shotgun sequence.
ACCESSION   DBNHOX010000023 DBNHOX010000000
VERSION     DBNHOX010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1223013"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /gene="msrP"
                     /locus_tag="AC16UZ_00480"
     CDS             <1..637
                     /gene="msrP"
                     /locus_tag="AC16UZ_00480"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017001639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HIR6245179.1"
                     /translation="GKPGTYDLDDLLKGLAPEERIYRFRCVEAWSMVVPWLGIPLKSI
                     LDRLEPLGSAKYVEFKTLYRPSEMRGQRSPFSSIDWPYVEGLRLDEANHPLTIMAVGL
                     YGKPLPNQNGAPWRLVVPWKYGFKSIKSIVSIRFTEREPENTWKLLQPQEYGFYANVN
                     PAVDHPRWSQASERRLPSSLFSQNRMPTLPFNGYADEVAGLYAGMDLTRYY"
     gene            642..1241
                     /locus_tag="AC16UZ_00485"
     CDS             642..1241
                     /locus_tag="AC16UZ_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013918.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016679 - oxidoreductase activity, acting
                     on diphenols and related substances as donors [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HIR6245180.1"
                     /translation="MRRRLLFTALCLPLAWLGYAIAGELNQPGSVLGADPGEAVVLFL
                     GEWGLRLLLLTLAVTPLRRLTGWAELLGYRRMIGLFSFTYLALHLLAYVALLAEFDWR
                     LVLEDFSDRAYITAGLIGFGLLLPLAVTSTRGWQRRLGRRWRSLHRLVYPAVFLGLLH
                     LVWLTKDGYGEVAAYGVLLSLLMVERIRHGAWWGRKAAG"
     gene            1243..1578
                     /locus_tag="AC16UZ_00490"
     CDS             1243..1578
                     /locus_tag="AC16UZ_00490"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR6245181.1"
                     /translation="MRCEPCRGDSPAVAPDAREAMLAELTGWRVDVVDEVPRLSRTYQ
                     TADFAAALGLANTIGEMADSEDHHPQLVVEWGRLQVSWWTHAIGGLHMNDFVLAARCD
                     ELAAKAASD"
     gene            complement(1575..2660)
                     /locus_tag="AC16UZ_00495"
     CDS             complement(1575..2660)
                     /locus_tag="AC16UZ_00495"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR6245182.1"
                     /translation="MKYLTDDVRIIGMEEVTAPETLLSELPITDSVSRLVFSNRKQIS
                     EILHGSDPRLLVVVGPCSIHDPEAALDYAKRLAPVAKDLSDRLHVIMRVYFEKPRTTV
                     GWKGLINDPHLDNSFDINNGLRIARGVLRDIAECGLGAGTEYLDPITPQYVGDLVAWG
                     AIGARTTESQIHRQLASGLSCPVGFKNSTDGSIQVAVDAIKSAANSHIFLSVTKSGHS
                     AIFSTAGNDDCHLILRGGGGKPNYDNPSVHYASRLLEQAGLTSRLMIDMSHANSGKDH
                     NQQIRVCQDICKQVREGESRIFGVMIESNLVPGNQKIGPRESMTYGQSVTDACIGWED
                     TVTCLTALADAGGARMEGSAAAAAGSG"
     gene            2770..3426
                     /locus_tag="AC16UZ_00500"
     CDS             2770..3426
                     /locus_tag="AC16UZ_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245183.1"
                     /translation="MTEAVRLDARRRGMLADMGIDVWVIRGNAAGVVEVPQSQTIVVP
                     EPVPPAETKAPEKGPDVARLNDSAPSAAQQAKMPVPRRRPLEHPEADVATEIAVTCLT
                     RPGALLLAYGREWTQAGRLGRDLLAAATGSWGSDPKRISFGWREPGDPSDGWRAFKAF
                     ADKQLADAEASVIFVNEALVDHLPERASSGTLVVLPNLAEFDTDAKRGLWTRIRNLGR
                     "
     gene            3396..3890
                     /gene="rimI"
                     /locus_tag="AC16UZ_00505"
     CDS             3396..3890
                     /gene="rimI"
                     /locus_tag="AC16UZ_00505"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR6245184.1"
                     /translation="MDQDPESRALINLRPMETADLDAVLEAEFLSYAFPWTRGVFLDC
                     LKARHECWVGCLGDAPVAHAVVSVGAGESHLLNLCVRRDLQGRGYGRALAVHVVGRAR
                     ARGASAMFLEVRPSNLVAAELYESLGFREIGIRKNYYPAQVGHEDARVLALDLDAHFR
                     SDPL"
     gene            3955..>4221
                     /locus_tag="AC16UZ_00510"
     CDS             3955..>4221
                     /locus_tag="AC16UZ_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016391145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR6245185.1"
                     /translation="MVATARTAALAGGSDRRTFAIISHPDAGKTTITEKLLLFGNAIQ
                     LAGTVKARKSDRHATSDWMAMEQQRGISVTTSVMQFPYRDRVVNL"
BASE COUNT          686 a         1283 c         1433 g          819 t
ORIGIN      
        1 gggcaaaccg ggcacctacg atctggatga tcttctcaaa gggctggcgc cggaggaacg
       61 catctatcgg tttcgctgcg ttgaagcctg gtcgatggtc gttccatggc ttggcattcc
      121 tctcaagtcc attctcgacc gccttgagcc cctcggcagc gcgaagtacg tcgagttcaa
      181 gacgctgtac cgccccagcg agatgcgtgg ccagcgctcg ccgttcagca gcatcgactg
      241 gccgtacgtg gagggcttgc ggctggacga ggccaatcac cccctcacga tcatggccgt
      301 tggcctctac ggcaagccgc tgccgaacca gaacggggcg ccgtggcgcc tcgtcgtgcc
      361 gtggaagtac ggcttcaaga gcataaagtc catcgtctcc attcgcttca ccgagcgtga
      421 gcctgagaac acctggaaat tgcttcagcc gcaggaatac ggcttctacg ccaacgtcaa
      481 tccggcggtt gaccacccgc gctggagcca ggcatcggaa cgacggctgc cgagctccct
      541 gttctcgcag aaccggatgc cgacgttgcc cttcaacggc tatgccgatg aggtggcggg
      601 tctgtatgcg gggatggatc tcacccgcta ttactgagcc cgtgcgcaga cgcctgctgt
      661 ttacggccct gtgcctgccg ctcgcgtggc tgggttacgc gatcgccggc gagctgaatc
      721 agcccgggag cgtgcttggg gcagatccgg gagaggctgt ggtgctcttt ctcggcgagt
      781 ggggcttgcg cctgcttctt ctcacactgg cggtcacgcc cttacggcgc ctgaccgggt
      841 gggcggagct gctcggttat cgccggatga tcggtctgtt cagcttcacc tacctggctt
      901 tgcacctgct tgcctacgtc gcgctgcttg ccgagttcga ctggcgtctg gtgctcgagg
      961 acttctctga tcgagcctac atcacggccg ggctcatcgg gttcgggctg ctgttgccac
     1021 tggctgtcac gtcgacccgc ggctggcagc gccgcctggg tcgccgctgg cgcagcctgc
     1081 accggctggt ctacccggcc gtgttcctcg ggctgctcca tctggtctgg ctcaccaagg
     1141 acgggtacgg cgaagttgcg gcatacggcg tactattgag tctattgatg gtagagagga
     1201 tcagacatgg cgcatggtgg ggacgcaaag cggctggctg agatgcggtg cgagccttgt
     1261 cggggcgatt cgccggcagt ggcgccggat gcgcgggagg ctatgctggc cgagctcacc
     1321 ggctggcggg tcgacgtggt ggacgaggta cccaggctca gccggaccta tcaaaccgcc
     1381 gacttcgccg cggcgctggg gcttgcgaac accatcggcg agatggccga cagtgaggat
     1441 caccatccgc agctggtggt ggagtggggc cggctgcagg tcagctggtg gacgcacgcc
     1501 atcggcggcc tgcacatgaa cgacttcgtt ctcgcggccc gatgcgacga gctggctgcc
     1561 aaggcggctt cggactagcc cgatcctgcc gctgcagcgg ccgagccttc catccgcgcc
     1621 ccgcccgcgt cggccagcgc ggtcaggcag gtgactgtgt cctcccaccc gatacacgca
     1681 tcagtgacgc tctggccgta cgtcatggat tcacgtgggc cgatcttctg gttgcccggg
     1741 accaggttgc tctcgatcat cacgccgaaa atccgtgact cgccttcgcg gacctgcttg
     1801 cagatatctt ggcagacccg gatctgctgg ttgtgatcct tgccgctgtt ggcgtggctc
     1861 atgtcgatca tcaggcggct cgttagcccc gcctgctcga gcagacggct ggcatagtgg
     1921 accgaagggt tgtcgtagtt cggcttgccg cccccgcctc gaagaatgag atggcaatcg
     1981 tcgttgccgg ccgtggaaaa gatggccgag tgcccggact tggtgacgga gagaaatatg
     2041 tgggaattgg cggccgactt gatggcgtcc acggcgacct ggatggagcc gtcggtgctg
     2101 ttcttgaaac ccacggggca ggacagcccg gaagcgagct ggcggtgaat ctggctctcg
     2161 gtggtgcggg ctccgatagc gccccaggct accaggtcgc cgacgtactg cggcgtgatc
     2221 ggatccaggt actccgtgcc cgcgcccagt ccgcattcgg ctatgtctct gagcacgccc
     2281 ctggcgatcc tcagaccatt gttgatgtcg aaggagttgt cgagatgggg gtcgttgatc
     2341 aggcccttcc accccacggt cgtgcgcggt ttttcgaagt agacccgcat gatgacgtga
     2401 agacggtcgg agaggtcttt cgccaccggg gccagccgct ttgcatagtc cagggcagct
     2461 tccgggtcat gaatcgagca gggcccgacg accaccagca atcgggggtc gctgccgtgg
     2521 agaatctcgc tgatctgctt gcggttggag aagaccagtc gcgaaacgga atcggtgatc
     2581 ggcagctcgg aaaggagggt ttccggcgcg gtgacctctt ccatgccgat gatgcgtacg
     2641 tcgtctgtca gatacttcat tgggaaccat cacggtgcgt gagctcggaa gctagagtgt
     2701 acaatgaccg cagaggcagg gttaccggca atcgcggcat gtataccaaa gttctgaacg
     2761 ggtgcgggca tgactgaagc cgttcgtctg gatgcgcggc gtcgggggat gctcgcggac
     2821 atgggcatag acgtctgggt tatcaggggt aacgcggccg gggtcgtcga ggtcccgcag
     2881 tcgcagacca tcgtcgtgcc cgagcccgtg ccgccggccg agacgaaggc gccggagaag
     2941 ggccccgacg ttgccaggct gaacgactcg gcgccttcgg cggcccagca ggcgaagatg
     3001 ccggtcccgc gccggagacc cttggaacat ccggaagcgg atgtcgcgac ggaaatcgcg
     3061 gtcacctgtc tgacgcgccc cggcgctttg ctgctggctt acggccggga atggacccaa
     3121 gccggcaggc tcggccgcga cctcctcgct gccgcgaccg gcagctgggg ctcggatcca
     3181 aagcgaatct cattcggctg gcgggagccg ggcgatcctt cggacggctg gcgggccttc
     3241 aaggcatttg cggacaagca gctggcggac gccgaggctt cggtgatttt cgtaaacgaa
     3301 gcgctggtcg atcaccttcc ggagcgggcg agctccggga cgctggtcgt gctgccgaac
     3361 ttagccgagt tcgacactga cgccaaacga ggcctatgga ccaggatccg gaatctcggg
     3421 cgctgatcaa ccttcggccc atggagacgg cggatctcga tgccgttctg gaagccgagt
     3481 ttttaagcta cgcgtttccg tggacgcgcg gggtctttct ggattgtctg aaggctcggc
     3541 acgaatgctg ggtcggctgc ctcggcgacg ctccggtggc gcacgccgtg gtgtccgtgg
     3601 gggccggcga gtcgcatctg ctcaatctgt gtgttcgcag ggaccttcag ggacggggtt
     3661 acggccgtgc tctagcggtg cacgtcgtgg ggcgggcccg ggcgcggggc gcgtcggcca
     3721 tgttcctcga ggtccggccc tctaatctgg tggccgccga actgtacgag tcgctcgggt
     3781 tccgggaaat cggaattcgc aagaattact atccggccca ggtgggtcat gaggacgccc
     3841 gggtactcgc gttggatctt gatgctcatt tccgcagcga cccgctataa tccgcgccgt
     3901 tttccggcgg ggccgtccgg cttcggaaaa gtcggtcgct gaggagtctt tatcatggtg
     3961 gcaacggccc ggaccgccgc gctcgcgggg gggagcgatc gacgcacctt tgcgatcatc
     4021 tctcatccgg atgccggcaa gaccacgatc accgagaagc tgctcctgtt cggcaacgcc
     4081 atccagctcg ccggcacggt gaaagcgcgc aagtcggacc gccatgcgac ctccgactgg
     4141 atggccatgg aacagcagcg ggggatttcc gtcaccacct cggtgatgca gtttccctat
     4201 cgggaccggg tggtgaacct g
//
LOCUS       DBNHOX010000024         2341 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_644797, whole genome shotgun sequence.
ACCESSION   DBNHOX010000024 DBNHOX010000000
VERSION     DBNHOX010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_644797"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="AC16UZ_00515"
     CDS             <1..797
                     /locus_tag="AC16UZ_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RHO alpha subunit C-terminal catalytic
                     domain-containing protein"
                     /protein_id="HIR6245186.1"
                     /translation="SRWLRVELECNWKFWSDNFNESYHTRTAHPQVAPIIDQDHFTSR
                     YEMFPMGHARIIQMGRPSLRDRVPEGQPHPWDKELRFWGIDPDGYPDYETKVMQGWLD
                     LKAAKRTRWKEKGFHHYEHLTDEELTESPFQTVFPNIAFGASADSFGVFRWEPHPTDP
                     EKCYFDIWSMTYPVEGIEETVHRTARNPLKFEEAEYEFRVYDNGQGVLDLSDQVVFQD
                     WMLNAGQTTGWHSRGYQEPYLAAQETRVRRFHEVLHDYLAGNPPGR"
     gene            872..1249
                     /locus_tag="AC16UZ_00520"
     CDS             872..1249
                     /locus_tag="AC16UZ_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245187.1"
                     /translation="MPRFVLIGLCEPPNPESQQQFDEWFVDQHIEDTAKCPNFIRGSV
                     YKLSGPHLDGETVSGYLSLYEVEAPSYPEAERVLNEWQANPEAWEGRKHHLATAQKLG
                     GMPLAIKGSGWYELLKSFDGPAA"
     gene            complement(1269..2060)
                     /locus_tag="AC16UZ_00525"
     CDS             complement(1269..2060)
                     /locus_tag="AC16UZ_00525"
                     /inference="COORDINATES: protein motif:HMM:NF021465.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HIR6245188.1"
                     /translation="MSRDPASEGRVSIRTLDQLALWSSLSIVVALSWWYLFLLAQDMA
                     DMAGMPMGDMAGMHPWTALDFLLMFLMWAVMMVGMMVPTAVRAIYIYARVASQAQARG
                     SPVAATQIFVAGYVLVWAAFSLGATALQAALDALGLLSPAMRSSSPLLGAGLLVAAGV
                     YQLTPWKDTCLRHCQSPAAYLAGRFGPRALDALSLGIRHGLYCLGCCWVLMLLLFLGG
                     VMNLLWIAAIAAFVLLEKVLPPALRVSRLAAVMMIASGAAYLMIA"
     gene            complement(2057..>2341)
                     /locus_tag="AC16UZ_00530"
     CDS             complement(2057..>2341)
                     /locus_tag="AC16UZ_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245189.1"
                     /translation="LYLPIEFEMDIAARRARLAIPGLIEARGEPMMDGFSGSEFHIAL
                     ARPKGSFEFTYAEIGQGFAEVHGELPMSLQGSYGQFCEHNYDHDGLVIPA"
BASE COUNT          468 a          797 c          739 g          337 t
ORIGIN      
        1 gctcccgctg gctgcgggta gagctcgagt gcaactggaa attctggtcg gacaacttca
       61 acgagtccta ccacactcgc accgctcatc cccaggtagc acccatcatc gaccaggatc
      121 acttcacctc gcgctacgag atgtttccca tggggcacgc gcgcatcatc cagatgggcc
      181 gcccgtcgct tcgcgaccgg gttcccgagg gccagccgca cccgtgggac aaggagctgc
      241 gcttctgggg catcgacccc gacggctatc ccgactacga gaccaaggtc atgcagggat
      301 ggctcgatct caaagccgcc aagcgcacgc gctggaagga gaaaggcttt caccactacg
      361 agcacctgac cgacgaggaa ctgacggaga gccccttcca gacggtcttt cccaacatcg
      421 ccttcggcgc ctcggccgat tcgttcggcg tcttccgctg ggagccgcac ccgaccgacc
      481 cggaaaagtg ctacttcgac atctggagca tgacctaccc ggtggagggc atcgaggaga
      541 ccgtgcaccg cacggcgcgc aacccgctca agttcgagga ggccgagtac gaattccgcg
      601 tctacgacaa cggccagggc gttctcgacc tctccgacca ggtggtcttc caggactgga
      661 tgctcaacgc cggccagacc acgggctggc attccagggg ctatcaggag ccgtacctgg
      721 cggcccagga aaccagggtc cggcggttcc acgaggtgct gcacgactac ctggcgggga
      781 acccgccggg gcgctgagcc gaggggtcac ggcgctctcc acacgagggc gctccgtccc
      841 ccttacgtat ttgacaagtc aggacacaca aatgccgaga ttcgttctca tcgggctctg
      901 cgagccgccc aaccccgaat cccaacagca gttcgacgag tggttcgtcg accagcacat
      961 cgaggatacc gccaagtgcc ccaacttcat ccggggcagc gtctacaagc tgagcgggcc
     1021 tcacctggac ggcgagaccg tttccggata cctctcgctc tacgaggtgg aggcccccag
     1081 ctacccggaa gccgagcggg tgctcaacga gtggcaggca aatccggagg cctgggaggg
     1141 acgcaagcac cacctggcca ccgcccagaa gctcgggggc atgccgctgg ccatcaaagg
     1201 ctcgggctgg tacgagctgc tgaagtcgtt cgacgggccc gccgcctgaa cgttagcgac
     1261 tcgagcggct aggcgatcat caggtaggcg gcgccgcttg cgatcatcat cacggcagca
     1321 agccgggata cacgcagcgc ggggggcagg actttctcca gcagcacgaa cgccgcgatg
     1381 gccgcgatcc acagcaggtt catcacgccg ccgagaaaga gcagcagcat cagcacccag
     1441 cagcagccaa ggcagtaaag gccgtgccgg atacccaggc tcagagcgtc cagcgctcgc
     1501 ggcccgaagc gtccggccag ataagcggcc ggggactggc agtggcgcag gcaggtgtcc
     1561 ttccacgggg tgagctggta cacgcccgcg gcaacgagca acccggcgcc cagcagcggg
     1621 ctggacgagc gcatggccgg tgacagcagg cccaatgcgt ccagcgcggc ctgcaacgcc
     1681 gtcgcgccga ggctgaaagc ggcccagacc agcacgtagc cggccacgaa gatctgggtg
     1741 gcggccaccg gcgagccccg ggcctgggcc tggctcgcaa cccgcgcgta gatgtagatg
     1801 gcgcgcaccg cagtgggcac catcatgccc accatcatca cggcccacat caagaacatg
     1861 agcaggaagt ccagcgcggt ccaaggatgc atgcccgcca tgtcgcccat gggcattccc
     1921 gccatgtcgg ccatgtcttg ggccagcagg aacaggtacc accaggacag cgctaccacg
     1981 atgctcagcg acgaccagag ggcaagctga tcgagggtcc ggatcgagac gcgcccttcc
     2041 gacgccggat ctcggctcac gcgggaatga cgagcccgtc gtgatcgtag ttgtgctcac
     2101 agaactggcc gtagctgccc tgcagcgaca tgggcagctc gccatgcacc tccgcgaatc
     2161 cctggccgat ctccgcatag gtgaactcga agctgccctt gggcctcgcc agcgcgatgt
     2221 gaaactcgct gccgctgaac ccgtccatca tgggttcgcc tctggcctcg atgaggccgg
     2281 ggatcgccag ccgcgcgcgg cgagcggcaa tgtccatctc gaactcgatg ggcaggtaga
     2341 g
//
LOCUS       DBNHOX010000025         4383 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1030356, whole genome shotgun sequence.
ACCESSION   DBNHOX010000025 DBNHOX010000000
VERSION     DBNHOX010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1030356"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1478)
                     /gene="ppk1"
                     /locus_tag="AC16UZ_00535"
     CDS             complement(<1..1478)
                     /gene="ppk1"
                     /locus_tag="AC16UZ_00535"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003918.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR6245190.1"
                     /translation="MSANPSTDFAGLANPELYINRELSLLEFHRRVLAQAQEERVPLL
                     ERLRYLCISSSVLDEFFEIRVAGLKQQEAYRATQRGPDNLSPSEQLRRIFETVRELVE
                     EQYRVLNEDLLPSLDDQGIRILDPEEWSSKQRQWLKRYFKRELAPIMSPIALDPAHPF
                     PQPLNKSLTFIVTLEGEDAFGRRSGKAIVQAPRALSRVVRLPESCAATPNEFVLLSSI
                     IEVHVGDLFPGMKSTGCFQFRVTRNSDLFVDMEEVDDLLRALEGELSSRRFGDAVRLE
                     VARDCPDPLEAFLTGQFHLKPEDVYRCSGPVNLMRLATVPDLVDRPELKFPGFTPGIP
                     DRLRNANDMFEVIRQGDVLLHHPFESFAPFIDFLRQAAADPQVLSIRQTLYRTGTESA
                     VVEALRDAATNGKEVLVVIELRARFDEEANIELANYLQRAGAQVVYGVVGHKTHAKMS
                     MVIRREGRPLRRYVHLGTGNYHTRTTRLYTDYGLFTCDPDIG"
     gene            1571..2299
                     /gene="cmoA"
                     /locus_tag="AC16UZ_00540"
     CDS             1571..2299
                     /gene="cmoA"
                     /locus_tag="AC16UZ_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003124239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HIR6245191.1"
                     /translation="MSSRDRVYRESKGQIVDFAFDEAVADVFPDMIRRSVPGYETVIP
                     ITGMLAARHLEHMPDERRLAYDLGCSLGATTTAVLDQLDERPARVIAVDNAAAMIARA
                     VDAIRDPRARFEVADVTMLEFEPAGVVLMNFVLQFIDPTQRVPLLKRIRQALEPDGLL
                     IVSEKLRFEDPYTQALFERAHLDYKRANGYSELEISQKRSALEKVMIVDTEAEHRSRF
                     EAAGFATTNRWFQCLNWVSYLVAP"
     gene            2296..3231
                     /gene="cmoB"
                     /locus_tag="AC16UZ_00545"
     CDS             2296..3231
                     /gene="cmoB"
                     /locus_tag="AC16UZ_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HIR6245192.1"
                     /translation="MIFEPVLDLIPEWRRLGPDLNARLSTKGHGDYPRWEAALAALPD
                     LQAEQAALSDRVTVSGNASEDQRRDLRESLMALHPWRKGPFSLFEVDIDCEWRSDWKW
                     LRVLPHLDSLSGRRVLDVGCGNGYYGWRMLEAGATAVFGVDPTLVFCMQHLAVSRYLS
                     GAANWVLPLRFEQLPPHPFDTVFSMGVIYHRRDPAEHARRLAEFTRPGGQVVVESLVV
                     EHGRSLKPVGRYARMRNVHVVPTTKRLARWLKDAGFVDPELVDLTPTTVAEQRATTWM
                     RFESLEDALDPKMRNRTIEGHPAPVRAVMVARKPV"
     gene            3253..3684
                     /locus_tag="AC16UZ_00550"
     CDS             3253..3684
                     /locus_tag="AC16UZ_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245193.1"
                     /translation="MRFETAYGLKTAKPQNTTMMLGIIPISFRFRGKIMRLEDMYHCF
                     REHPGTGLHPTQISRYTGLGLAEVNARLEATPELFVRLPKRPDGITRYRLTSATTAKA
                     PEDVEKFLRQAARRESLMLYAVGSMVLLILLIVVILVGPAV"
     gene            3685..>4383
                     /locus_tag="AC16UZ_00555"
     CDS             3685..>4383
                     /locus_tag="AC16UZ_00555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6245194.1"
                     /translation="MPELLDDKQRALREHAAAFAREQLEPRRSMDNKQAREEIVRAAK
                     AAGFYAMTQPAEFGGNPASVLELTIVRDELAASNAPHADAVFGPGPGVLAGVGEPLAS
                     DYLGPVLAGTKQGAFGFTEPDDAPGPTSGRLESDELIVSGQKSYVTGGEQADFINTLV
                     QIEGHGPALVVIDTHAAGVIRERTFSSLDGSRHAAFRFEEVRVPRSHVIGKPGEGLPR
                     AMRQIGDTRLAIAAQ"
BASE COUNT          809 a         1431 c         1389 g          754 t
ORIGIN      
        1 tcgccgatgt cggggtcgca ggtgaacagg ccgtagtcgg tgtaaagccg cgtggtgcgc
       61 gtgtggtagt tgccggtgcc cagatgcaca tagcggcgca gcggacggcc ttcccgacgg
      121 atgaccatgc tcatcttcgc gtgggtcttg tgccccacca cgccgtaaac cacctgtgcg
      181 cccgcgcgct gaaggtagtt ggcgagctcg atgttcgcct cctcgtcgaa acgcgcacgc
      241 aactcgatga ccaccagcac ttccttgccg ttggtcgccg cgtcccgcag cgcttccacc
      301 accgccgact ccgtccccgt gcgatagagc gtctgacgga tcgaaagcac ctggggatcg
      361 gcggccgcct gtcgcagaaa gtcgatgaaa ggcgcgaacg actcgaaagg gtgatgcagc
      421 agcacgtctc cctgacggat gacctcgaac atgtcgttgg cgttacgcag ccgatcggga
      481 atacccggcg tgaaccccgg aaacttcagc tccgggcgat cgacgaggtc cggaacggtc
      541 gccaggcgca tcaggttgac ggggccactg cagcgataga cgtcttcggg cttgaggtgg
      601 aactggccgg tgagaaaagc ttccagagga tcgggacagt cccgggcgac ttcgaggcga
      661 acggcatccc cgaaccggcg cgaggaaagc tcgccttcga gcgcccgcaa cagatcgtcg
      721 acttcttcca tgtcgacgaa aagatcgctg ttgcgcgtca cgcgaaactg aaaacagccc
      781 gtgctcttca tgcccgggaa caggtcaccg acgtgcacct cgatgatcga cgagagcaga
      841 acgaactcgt tcggcgtggc ggcgcaggac tccggcaggc gcaccaccct ggagagcgcg
      901 cgaggtgcct gaacgatggc tttgccgctg cggcggccga aggcgtcttc gccttcgagg
      961 gtgacgatga aggtgaggct cttgttcagc ggctggggga aggggtgcgc ggggtcgagc
     1021 gcgatggggc tcatgatcgg cgcgagctcc cgcttgaagt accgcttgag ccactgcctt
     1081 tgcttgctgc tccactcttc cggatccaga attcggatgc cctggtcgtc gagactcgga
     1141 agcagatctt cgttgagaac ccggtactgt tcttcaacaa gctctcggac ggtctcgaag
     1201 atgcgccgca gctgctcgct gggagacagg ttgtccggtc cccgctgtgt ggcgcgatac
     1261 gcctcctgct gcttgagacc ggcgacccgg atctcgaaaa actcgtcgag caccgagctc
     1321 gatatgcaca gatagcgcaa gcgttccaag agcggcacgc gctcttcctg ggcctgggcg
     1381 agcacgcggc gatgaaactc tagcagcgag agctcacgat tgatgtagag ctccggattc
     1441 gcgagcccgg cgaagtccgt ggaagggttt gcgctcaact gagactctga ctaacaattg
     1501 acaccgcatt ttaccatgcg cgccggaggc caatgttaaa gcttcgtgtt gccgacgggg
     1561 ggccgcctcc ttgagttccc gcgaccgcgt ctaccgcgaa tcaaaaggac agatcgtcga
     1621 cttcgccttc gacgaagccg tggccgacgt gtttcccgac atgattcgcc gctccgtccc
     1681 cggctacgaa accgtgatcc cgatcacggg aatgctggcg gccaggcatc tcgagcacat
     1741 gcccgacgag cgccgtctgg cttacgacct gggttgctcg ctgggcgcca ccaccaccgc
     1801 cgtcctggac caactggacg aacggcccgc tcgcgtcatt gccgtggaca acgctgcggc
     1861 catgatcgcc cgagcggtcg acgccatccg tgacccgcgg gcgcgcttcg aggttgccga
     1921 cgttaccatg ctggagttcg aacctgccgg cgtcgtgctg atgaacttcg tgctccagtt
     1981 catcgacccc acgcaacggg tgccactcct gaaacgaatc aggcaagcgc tcgaaccaga
     2041 cggcttgctc atcgtctccg agaagctgcg cttcgaagat ccttacaccc aggccctgtt
     2101 cgagcgggcc cacctcgact acaagcgtgc caacggatac tctgagctcg agatcagcca
     2161 gaaacggtca gcactggaaa aggtgatgat tgtcgacacc gaagcagagc atcgcagccg
     2221 cttcgaagcc gccggattcg caaccaccaa cagatggttc cagtgcctga actgggtttc
     2281 ctacctcgtt gccccatgat ctttgagccc gtcctggatc tcatacccga atggcgccgg
     2341 ctcggcccgg acttaaacgc gcgcctgtcg acgaaggggc acggcgacta cccgcgctgg
     2401 gaagccgcgc tcgcagcgct gcccgacctc caggcagagc aggccgccct cagcgatcgg
     2461 gtaacggtga gcgggaacgc ctccgaagac cagcggcgcg acctgcggga atcgctgatg
     2521 gccctgcatc cgtggcgcaa gggacccttc agcctgttcg aagtggacat cgactgcgag
     2581 tggcgctcgg actggaagtg gctgcgcgtg ctcccccacc tggattccct ttccgggcgc
     2641 cgcgtcctcg acgtgggatg cggcaacggc tactacggct ggcgcatgct ggaagccggc
     2701 gccaccgcgg ttttcggcgt ggatcccacg ctggttttct gcatgcagca tctggcagtc
     2761 agccgctacc tgtccggcgc ggccaactgg gtgctgcccc tgaggttcga gcagcttcct
     2821 ccgcatccgt tcgataccgt tttcagcatg ggcgtgattt atcaccgacg ggatcccgcc
     2881 gagcacgccc gccggcttgc ggagttcacg aggcccggtg gccaggtcgt ggtggaatca
     2941 ctcgtggtcg agcacggccg atctttgaag cccgtaggcc gctacgcgcg aatgcgcaac
     3001 gttcacgtgg tgcccacgac gaaacggctc gcgcgctggc tcaaggacgc gggcttcgtc
     3061 gatccggagc tcgtcgacct gacacctacc acggtcgccg agcagcgcgc gacgacgtgg
     3121 atgcgattcg aatccctcga ggacgctcta gatcccaaga tgcggaaccg aaccatcgaa
     3181 ggtcatcccg cccccgtccg agccgtcatg gtcgcgcgca agccggtctg attcgcgccg
     3241 gcgggcgtgg aaatgcgctt tgaaaccgcg tacggtctca agacggcaaa gccgcagaac
     3301 acaaccatga tgctcgggat cattcccatc agttttcgct tccgcggcaa gatcatgcgg
     3361 ctggaggaca tgtatcactg ctttcgggag catcccggaa ccgggctgca tccgacgcag
     3421 atatcccgct atacgggcct gggtctggcc gaggtgaacg ccagactgga agcgactccc
     3481 gagctcttcg tgcgccttcc gaaacgcccc gacggcataa cgcgctaccg gctgaccagt
     3541 gccaccacgg caaaggcgcc cgaggacgtt gagaagttcc tccgccaggc ggccaggcgc
     3601 gaaagcctga tgctttacgc cgtgggaagc atggtgctgt tgattctgct catcgtcgtc
     3661 atcctcgtcg ggcctgccgt ctagatgccc gaactgctcg acgataagca gcgcgcgctt
     3721 cgcgagcacg ccgcggcctt cgcccgtgag cagctggaac ccagacgcag catggacaac
     3781 aagcaggcgc gtgaggaaat cgtccgcgct gccaaagccg ccggtttcta cgccatgacc
     3841 cagcccgcgg agttcggcgg caacccggcg tcggttctgg aactcacgat cgtcagagac
     3901 gagttggcgg cgagcaacgc gccccacgcc gatgcagtgt tcgggccggg tccgggggtg
     3961 ctggccggag tgggagagcc cctggctagc gactaccttg ggcccgtgct ggcgggcacc
     4021 aagcaaggcg cgttcggttt caccgagccc gatgacgccc ccggaccaac cagcgggcgg
     4081 ctggaatcag acgagctgat cgtcagcggg cagaagtcgt acgtcaccgg cggcgagcag
     4141 gccgacttca tcaacaccct cgtgcagatc gagggccacg gccccgcgct ggtcgtgatc
     4201 gatacccatg ccgccggggt gatccgcgag cgaaccttct cgtcgctaga cggcagccgt
     4261 cacgccgcct ttcgattcga agaagtgcgc gtgccccgct cgcacgtgat cggcaagccc
     4321 ggcgaggggc tgccgcgagc catgcgccag atcggcgata cgcgcctggc catcgccgcc
     4381 cag
//
LOCUS       DBNHOX010000026         2638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_516337, whole genome shotgun sequence.
ACCESSION   DBNHOX010000026 DBNHOX010000000
VERSION     DBNHOX010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_516337"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1722
                     /gene="istA"
                     /locus_tag="AC16UZ_00560"
     CDS             223..1722
                     /gene="istA"
                     /locus_tag="AC16UZ_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043767605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HIR6245195.1"
                     /translation="MYSVELYRRVRRACHVEGMSERAAARHFGIDRKTVSKILKHSVP
                     PGYQRERPPVRPKLDPFIPTIDRILEEDRGRLKKQRHTAKRIFERLRDEHGFAGGITI
                     VTDYVREKKRRTREVFVPLAHAPGHAQVDFGEALGEIGGVVRKLHYFAMALPHSDAFF
                     IKAYPAETTEAFCDGHVSAFAFFGGVPHSILYDNTTLAVAKILGDGTRKRTRSFAELQ
                     SHYLFEDRFGRPGKGNDKGHVEGVIGFGRRNFLVPMPRFESFEALNVWLEEQCLKRQD
                     ADLRGHSETIGERLMRDLDALMALPPAPCDACERIGTRATSISMVRYRSNDYSVPVAY
                     AHHEVQVRGYVGEVVIGSGTEIIARHRRSYDKADMVFDPMHFLPLLEQKVGALDQAAP
                     LKGWELPGEFATLRRLLEARMGKAGKREYVQVLRLLETFEINHVHGAVSQALNLGAIG
                     YDAVKHLVLCRIERRPPRLDLDIYPYLPRARVETTRPASYMSLMPGAAA"
     gene            1719..2471
                     /gene="istB"
                     /locus_tag="AC16UZ_00565"
     CDS             1719..2471
                     /gene="istB"
                     /locus_tag="AC16UZ_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HIR6245196.1"
                     /translation="MSDTPQVLLQHHLKKLKLPTFQSEYAKLARQCAAEGKDHVQYLL
                     RLCELELIERERRMIERRVKAAKFPTTKSLDSFDFKAVPSLNKVLTMELARCEFVERR
                     ENVIALGPSGTGKTHVALGLGLAACQKGLKVRFTTAAALVHELIEAADERRLQRLQKL
                     MASQDLLIIDELGFVPLSKSGAELLFEIISQRYERGSIIITSNLPFDEWTEVFGSERL
                     TGAILDRLTHHVHILEMNGESFRLRQSRKAKA"
BASE COUNT          494 a          862 c          851 g          431 t
ORIGIN      
        1 acgaagtgcg cgacatgttc ctgcggctcc agaatggaac gacgttgaag gcccaggaga
       61 agcgaaatgc aatgacaggc caaatgcggg atttcgttaa gaagatcgct ggtgtaaatc
      121 ccggagacaa actggaccac ggaatcggtc ggatagcctg atcgtcgcgg agtaaaattc
      181 cgccaccttg acgcctgatg cgaacgagct gaaggcgggg agatgtattc tgtggagctg
      241 tacaggcggg tgcgccgggc gtgccatgtc gaggggatga gcgagcgggc agcggcccgg
      301 cattttggga tcgaccgcaa aaccgtttcc aagattttga agcattcggt gccgccgggg
      361 tatcagcgtg agcgcccccc ggtccggccg aagctcgatc cgttcatccc gaccatcgac
      421 cggatccttg aagaggatag gggccgactg aagaagcagc ggcataccgc gaagcggatc
      481 ttcgagcgtc tgcgtgacga gcacgggttc gccggcggca tcaccatcgt caccgactat
      541 gtgcgggaga agaagcgccg gacgcgcgag gtgttcgtgc cgctggcgca tgccccgggc
      601 cacgcgcagg tggacttcgg cgaggcgctc ggggagatcg gcggggtggt gcgcaagctg
      661 cactactttg cgatggcgct gccgcactcg gatgcgttct tcatcaaggc ctatccggcg
      721 gagacgacgg aggcgttctg cgatggccat gtttcggcct ttgccttctt cggcggcgtg
      781 ccgcactcca tcctctacga caacaccacg ctcgctgtgg caaagatcct gggcgatggg
      841 acccgcaagc gcaccaggtc cttcgccgag ctgcagtcgc attatctgtt cgaggaccgg
      901 ttcggccgtc cgggcaaagg caacgacaag ggccatgtcg agggcgtgat cggctttggc
      961 cgacggaact tcctggtgcc gatgccacgc ttcgagagct tcgaggcgct gaacgtctgg
     1021 ctggaggagc agtgcctcaa gcgccaggac gcggacctgc gcgggcacag cgagaccatc
     1081 ggcgagcggc tgatgcgcga cctcgacgcc ctgatggcgc tgccgccagc cccctgcgac
     1141 gcctgcgaga ggatcggcac ccgggcgacc tcgatctcga tggtgcgcta ccgcagcaac
     1201 gactactcgg tgccggtggc ttatgcccat cacgaggtgc aagtccgcgg ctatgtcggc
     1261 gaggtcgtca tcggcagtgg gacagagatc atcgcccgcc accggcggtc ctacgacaag
     1321 gccgacatgg tgttcgaccc catgcacttc ctgccgctgc tggagcagaa ggttggtgcg
     1381 ctcgaccagg ccgcaccgct gaagggctgg gaactgcccg gcgagttcgc caccctgcgc
     1441 cggctcctgg aggcgcggat gggcaaggcg ggcaagcgcg aatacgtgca ggtcctgcgg
     1501 ctgctcgaga ccttcgagat caaccatgtg catggcgcag tcagccaggc actcaatctc
     1561 ggggccatcg gctacgatgc ggtcaagcat ctggtgctct gccgcatcga gcgccgcccg
     1621 ccacggcttg atctcgacat ctatccctac ctgccgaggg cacgggtcga gaccaccagg
     1681 ccggccagct acatgagcct gatgcccggg gcggcggcat gagcgacacc ccgcaggtgc
     1741 tgctgcagca ccacctcaag aagctcaagc tgccgacctt ccagtccgag tacgccaagc
     1801 tcgcccggca gtgcgccgcc gagggcaagg accatgttca gtatctgctg cggctctgcg
     1861 agctggagct gatcgagcgc gagcggcgga tgatcgagcg gcgcgtcaag gccgcaaagt
     1921 tccccaccac caagagcctc gacagcttcg acttcaaggc cgtcccgtcg ctgaacaagg
     1981 tgctgacgat ggagctcgcc cgctgcgagt tcgtcgagcg ccgcgagaac gtcatcgcct
     2041 tggggccgag cggcaccggc aagacccatg tcgccctcgg tctcggcctg gcggcctgcc
     2101 agaagggcct gaaggtccgc ttcaccaccg ccgccgccct ggtccacgag ctcatcgaag
     2161 ccgccgacga gcgccgcctg cagcgcctgc aaaaactcat ggcgagccag gacctgctga
     2221 tcatcgacga gctcggcttc gtccccctca gcaagtccgg cgccgagctc ttgttcgaga
     2281 tcatctccca gcgttatgaa cgcggctcga tcatcatcac ctcgaacctg cccttcgacg
     2341 aatggaccga ggtcttcggg tccgagcgcc tcaccggcgc catcctcgac cgcctgaccc
     2401 accacgtcca catcctcgag atgaacggcg agagcttccg cctgcgccag agccgcaagg
     2461 ccaaagcctg acctcacaat agactgacgg catcagaact ggccttcggc cagtgcgcct
     2521 tgtcgtacgt cagctatatg gggagcacgc ccgccaaggc gcgtcgcgga caccaaccga
     2581 atggcccact attccaccgg cgcgtggccc cattttgcgc cgggattgac cgaggcct
//
LOCUS       DBNHOX010000027         4050 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1352020, whole genome shotgun sequence.
ACCESSION   DBNHOX010000027 DBNHOX010000000
VERSION     DBNHOX010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1352020"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="AC16UZ_00570"
     CDS             <1..1140
                     /locus_tag="AC16UZ_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6245197.1"
                     /translation="PFFWYEALRAGGPVRRMPDGSMLVTGYDACSDIYRNEAFISDKR
                     NLFRPKFGDSPLYEHHTTSLVFNDPPYHTRVRRTIVEALKPRSIQPTLDMLARRIQEL
                     LASMGEAGEADLIEAYASRIPVEVICSLLTVPTQDRDDLRRWSLAILGALEPAISESE
                     AEQGNRAVTEFLGYLRTLIAERRRRPLAEDSVLSSLLAQQDRGEVSELELLHNCIFLL
                     NAGHETTTNLIGNGVHMLLTHDSERDLLVAHPSRIKSAVEEILRYQSPNQLGNREVTA
                     NVTMCGQRFAPGDQVTLCIAAANRDPRKFPDAHRFLIDRAPNPHLAFAAGPHTCAGMA
                     LARIEGKLALSAFFQAFPEATLQARPRYRDRIRFRGLEALHVRLQ"
     gene            1225..2124
                     /locus_tag="AC16UZ_00575"
     CDS             1225..2124
                     /locus_tag="AC16UZ_00575"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6245198.1"
                     /translation="MTEAFDVVGDWQVARLAGALGAEVRGIDLRQVTSEEVDRIRSLL
                     LDHLVLFFPDQNISVDDHVSFGRYFGELEGHPHLDNPFTEHPELFELAASRGGVADEW
                     HTDITFRDEPALMSILHMVRCPTVGGDTMWSSLYAAFEELSEPMRDLCEGLTALHDAL
                     PHGRPEQTAIHPVVRVHPETGRKALYVNEHFTRRIVEMNARESDMLLGYLTKWVQNPR
                     FTVRYRWAEGTIAMWDNRCTQHFVLNDFEGERVIQRVTVMGDRPEGSGARWAPAVRTG
                     RHSATSRYDAQLVRHLRSREDQA"
     gene            complement(2224..3777)
                     /locus_tag="AC16UZ_00580"
     CDS             complement(2224..3777)
                     /locus_tag="AC16UZ_00580"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6245199.1"
                     /translation="MTDPLTELARSQPDKPALIDDRPDGTLLKWTYSELEERTNALAR
                     VFQNAGVEAGNRLIWCGPNSAWVVAAGIAARKIGAVAVPLNYRLTAEEAAYVVDNSDA
                     VTVFADAEYADLFAEIRDDIPQVREVLIFDGPAGPGQTSAEERLAQEGTEELAVKVDP
                     TTSGTMIYTSGTTGRPKGAVRKDTGDPALIAALMELMQLRGDDVYLTTGPLYHSGPSG
                     FMAFAFAFGNTIVLQRKFDAEDWLRLIDKYGVSFTFSAPTPVRLATQLPPETIKRYDL
                     SSVRITIANAAPWSFALKKAYLEHFPADSLFEVYGSTELGVNTIMLPADQLRKPGSCG
                     QAAPGVEIILIDEEGREVTEPKVPGEVFIRSPSVFSTYHKAEDKYLEDRRGDMQTVGD
                     IAYRDDEGFYYICDRKKDMIISGGVNVYPAEIEAALEHHPDILDVAVFGIPSEEWGES
                     VHAVVVKRSGSGLNEEAIQAFAREHLAGYKVPRSIEYRDAIPRTGTGKILKRELRAPW
                     WEGHNSSVS"
BASE COUNT          681 a         1285 c         1296 g          788 t
ORIGIN      
        1 ccctttttct ggtacgaagc gcttcgcgcc ggcggtcccg tccgccggat gcccgacggg
       61 tcgatgctgg ttaccggata tgacgcgtgc tcggacatct atcggaacga ggcgttcatt
      121 tcggacaaac gaaacctctt ccggccgaag ttcggagact cgccgctgta cgagcatcac
      181 acgacgagtc tggtcttcaa cgaccctccg taccacacgc gggttcggcg gaccatcgtg
      241 gaggcgttga agccgcgctc gatccagccg accctagaca tgctcgcccg gcgcattcag
      301 gaactgctcg catcgatggg ggaggcggga gaggcggacc tcattgaagc gtacgcgtcc
      361 cggattccgg tggaagtcat ctgcagcctg ctcacggtgc cgacgcagga cagggacgac
      421 ctgcggcgct ggtcgctggc cattctgggt gcgctggagc ccgctatctc cgaatccgag
      481 gcagagcaag gcaaccgcgc ggtaacggaa tttctggggt atctcaggac gctgatcgcc
      541 gagcgtcggc gccgccccct cgccgaggac agcgttctgt cctcgctcct ggctcagcag
      601 gatcggggcg aggtgagcga gctcgaactg ctgcacaact gcatctttct tctgaacgcc
      661 ggacacgaga ccacgaccaa tctgatcggc aatggcgttc acatgctgct gacccacgac
      721 tccgaaaggg acctgctggt tgcgcatcct tcacgaatca agtcggcggt ggaggagatc
      781 ctgcgctatc aaagccccaa ccagctcggg aaccgtgagg tgacggcgaa cgtaacgatg
      841 tgtgggcaac gctttgcgcc gggtgatcag gtgaccctgt gcatcgcagc ggcgaatcgc
      901 gacccgcgca aatttccgga tgcccatcgt ttcctgatcg atcgcgcgcc gaatccccac
      961 ctggcctttg ccgcgggtcc ccatacctgc gcgggcatgg cccttgcgcg aatcgaggga
     1021 aagcttgcgc tcagcgcatt tttccaggcg tttcccgaag cgacccttca ggcgcggcca
     1081 cggtatcgtg atcggatccg cttccgcggc ctggaagcgc tccacgtccg cttgcagtga
     1141 acgcggcaca aggggccttg gcggcttgca ggcccggaag cgttatatac tctgcctacg
     1201 tgacgcggaa tctgatggac gatcatgact gaagctttcg atgttgtcgg cgattggcag
     1261 gtggcccggc tggctggcgc cctgggcgcg gaggttcgcg gaatcgatct gagacaggtg
     1321 acgtcggagg aggtggaccg gatccggagc ctgttgctcg accacctggt cctgttcttt
     1381 cccgatcaga acatcagcgt agacgaccac gtctcgttcg gacggtattt cggtgagctc
     1441 gagggccatc cccatctgga taacccgttc accgagcacc ccgagctctt cgagctggca
     1501 gcgagccgcg gcggcgtcgc cgacgagtgg cacacggaca tcacttttcg ggacgagccc
     1561 gcgcttatgt cgattctgca catggtgcga tgcccaaccg ttggcgggga caccatgtgg
     1621 tcgagccttt acgcggcgtt cgaggaactc tcggaaccca tgcgcgatct ctgcgagggc
     1681 ctcacggccc tgcatgacgc gctgccccat ggccgcccgg agcagacggc cattcatccg
     1741 gtggtccgcg tgcatccgga aaccgggcgc aaggcgcttt acgtgaacga gcatttcacc
     1801 cggcgaatcg tggagatgaa cgccagggaa agtgacatgc tgctgggcta tctcacgaag
     1861 tgggtgcaga acccccgttt caccgtgcgc taccgctggg cggagggaac catcgccatg
     1921 tgggacaacc gctgcaccca gcacttcgtt ctgaacgatt tcgaagggga acgcgtcatt
     1981 cagcgggtga ccgtgatggg agaccggccg gaaggcagcg gggcgcgttg ggctccggcg
     2041 gtccgcacgg gccggcactc tgccaccagc cgttacgacg cccagctcgt tcgccatctg
     2101 cgcagccggg aggatcaggc ttagcgccgg ccgcgcctgg gccgcttccg gtgcagggtc
     2161 gttcgcctct ttcgccttgg ggcacccgcc gcgcgatggt cttcacgtca ccgggctgcg
     2221 gttctacgaa acgctgctgt tgtgcccttc ccaccagggc gcgcgcagct cccgcttgag
     2281 gatcttgccg gttcccgtgc gagggatcgc gtcgcgatat tcgatcgagc gcggaacctt
     2341 gtagcccgcc aggtgctccc gggcgaatgc ctgaatggcc tcctcgttca atccagagcc
     2401 gctgcgtttc acgacgacgg cgtgcacgct ctctccccac tcctcgctgg ggattccgaa
     2461 cacggcgacg tcgagtatgt cggggtgatg ctccagcgcg gcttcgatct ccgccggata
     2521 gacgttgacc ccgccggaga tgatcatgtc cttcttgcga tcacagatgt agtagaagcc
     2581 ttcgtcgtcg cgataggcga tgtcgcccac cgtctgcatg tcgccgcggc ggtcttccag
     2641 atacttgtcc tctgccttgt gataggtgga gaacacgctc ggcgagcgga tgaacacctc
     2701 gccggggact tttggttccg tgacctcccg gccttcctcg tcgatgagaa tgatctccac
     2761 accgggcgcg gcctgaccgc aggaccccgg ctttctgagc tggtccgcgg gcagcatgat
     2821 ggtgttcacg ccaagctcgg tggacccgta aacctcgaac aggctgtcgg ccggaaagtg
     2881 ctcgaggtag gcctttttca gcgcgaagct ccacggagcc gcgttggcga tggtgatgcg
     2941 cacgctcgag aggtcatagc gcttgatggt ctcgggcggc aactgcgtgg ctagccttac
     3001 gggcgtcggc gcgctgaagg tgaaggagac cccatacttg tcgatgagtc gcagccagtc
     3061 ctcggcgtcg aatttgcgct gcaggacgat ggtgttgccg aaggcgaacg caaacgccat
     3121 gaagccgctg ggtccggagt gatagagcgg cccggtggtc aggtagacgt cgtcgcccct
     3181 gagctgcatc agttccatca gcgcggcaat cagggccgga tcgccggtgt ccttgcgcac
     3241 cgcgcctttc ggacgaccgg tggtcccgga ggtgtagatc atggtgccgg aggtcgtcgg
     3301 gtcgactttc actgcgagtt cctcggtgcc ctcttgagcc aggcgctcct cggccgacgt
     3361 ctgtcccggt ccagccggac cgtcgaatat caggacctcc cgaacctggg ggatgtcgtc
     3421 ccgaatctcg gcgaacaggt cggcgtactc ggcatctgcg aaaaccgtca cggcgtcgga
     3481 gttatccacc acgtaggcgg cttcctctgc ggtcagccgg tagttcagcg gcacggcgac
     3541 tgcgccgatc ttgcgcgcgg cgatgccggc cgcgaccacc catgcagaat tgggcccgca
     3601 ccagatcaac cggttgccgg cttccacgcc ggcgttctga aagacccgtg cgagggcgtt
     3661 cgtgcgctct tccagctctg agtacgtcca cttcagcagg gttccgtcgg gccggtcgtc
     3721 gatcagcgct ggcttatccg gttggctgcg cgccagctcg gtcaacggat ccgtcatctc
     3781 gctctcctcg tgccccgaat gagtttcccg ggctgatggt agccgggcgt tcgtggtcgt
     3841 gcatccgcag ccattcgtaa ccgacgggca gcaggcgtag aattctccgg gaacgaacga
     3901 acttcgggga gtgtgcttat ggccagcaat ccgcaatctg gggatgtctc tgacatccag
     3961 ctgttcgacg tagaaaccct caaggacccc tgggatgcct atgcccgcct gcgcgatcac
     4021 gcccccgttt tcttcgtacc tggttttgat
//
LOCUS       DBNHOX010000028         1580 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_902313, whole genome shotgun sequence.
ACCESSION   DBNHOX010000028 DBNHOX010000000
VERSION     DBNHOX010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_902313"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="AC16UZ_00585"
     CDS             complement(<1..946)
                     /locus_tag="AC16UZ_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003210351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6245200.1"
                     /translation="MSFGIESTTDQVLAGVDLAGKRAIVTGASGGLGEETARALAAKG
                     AAVTITARDLAKGEAAAQKIRESTGNAAVDVRGLELADLASVRAFAAGYLADHDKLDL
                     LINNAGVMACPLTRTPAGWEMQFATNHVGHFLLTCLLAPALKAAAPSRVVNLSSAGHH
                     MSAVDLDDPFFNDREYDKWVSYGQSKTANILFSVGLEKRLAGAGVHAYAVHPGGIMTE
                     LGRHLTDEDIAAMMERNNISDPEAFQAGFKTVPQGAATSVWAATSPELEGRGGLYCED
                     CHVAGPGAGGIRDGGYAPWALDPDAAERLWALSEELVDQ"
     gene            1144..>1580
                     /locus_tag="AC16UZ_00590"
     CDS             1144..>1580
                     /locus_tag="AC16UZ_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015016 - heparan sulfate
                     N-sulfotransferase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HIR6245201.1"
                     /translation="MVSGQDHRRFLKTHSPLDGLPYYPECHYLVVFRDPRDMFFSMLK
                     HRDNFTDEELAFASIPSGEDAFRQWLDRGFDPKNFDFQSLEWLCHFLQTYWPYRDLPN
                     IHLFHYSDMKNDLPGHVRKVAGILEVELTEAQLDEMTAAASFEA"
BASE COUNT          266 a          508 c          493 g          313 t
ORIGIN      
        1 gctgatccac cagttcctca gacaaggccc acagccgttc ggccgcgtcg ggatcgagtg
       61 cccagggcgc gtaaccgccg tcgcggatgc caccggcgcc gggtccggcc acgtggcagt
      121 cctcgcagta gaggccgccg cggccttcca gctcgggtga ggtggcggcc cacacgctgg
      181 tcgccgctcc ctgggggacc gtcttgaagc cggcctgaaa ggcttcggga tcgcttatgt
      241 tgttgcgctc catcatggcg gcgatgtcct cgtccgtcag atgccggccg agctcggtca
      301 tgatgccgcc gggatgtacc gcgtaggcgt gaacgcccgc gccggccagg cgcttttcca
      361 gcccgacaga gaacaggatg ttcgcggtct tcgactgccc gtaggacacc cacttgtcgt
      421 actcacggtc gttgaagaac gggtcgtcca gatccaccgc gctcatgtga tgcccggcgg
      481 agctcaggtt gacgacgcgc gacggtgccg ccgccttcag cgccggggca agcaggcagg
      541 tcagcaggaa gtggccgacg tgattggtgg caaactgcat ctcccatccc gctggggtac
      601 gcgtgagcgg gcacgccatg acaccggcgt tgttgatcag cagatccagc ttgtcgtggt
      661 cggccagata ccccgcggcg aaagcccgca cgctggcgag atcggcgagc tccagcccgc
      721 ggacgtctac ggctgcgtta ccggtgctct cgcggatttt ctgcgcggcg gcctcgccct
      781 tggccaggtc ccgggccgtg atcgtgacgg ccgcgccttt tgcggccagg gcccgagcgg
      841 tttcctctcc caggccgcca gaagcgcccg tcacgatggc gcgtttgccg gcaaggtcga
      901 cgcccgccag cacttggtcc gtggtgcttt caatgccaaa gctcatttcg tgtccctcaa
      961 tttcttgata cgccggatga taacgccaat atggtcggat acgtgtcttt cgcccgaatg
     1021 tcccgttatt ctccgtgagg gcgctcggtc agagcaggag aaatcgatgg gggagcctct
     1081 gccatcgcgc tcggctcgat acatcgggcg cgtcaccgat acactatcga ggagtacctg
     1141 gagatggtct cggggcagga tcaccggcgt ttcctcaaga cgcactcgcc gctggacggg
     1201 ctgccctact atccggagtg tcattatctg gtggtgtttc gagatccccg agacatgttc
     1261 ttttccatgc tcaagcaccg cgacaacttc accgacgagg agcttgcatt tgcctccata
     1321 ccgagcggtg aggacgcttt tcggcaatgg ctggatcgtg gcttcgaccc gaagaacttc
     1381 gactttcagt cgcttgagtg gctgtgccac tttctgcaga cctactggcc ctacagggat
     1441 cttccgaaca tccatctttt ccattactcg gacatgaaga acgacctgcc gggacacgtc
     1501 cgcaaagttg ccggtatcct cgaagtagag ctgacggaag cccagctcga cgagatgacc
     1561 gcggccgcct cgttcgaagc
//
LOCUS       DBNHOX010000029         2192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1159504, whole genome shotgun sequence.
ACCESSION   DBNHOX010000029 DBNHOX010000000
VERSION     DBNHOX010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1159504"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /gene="gatB"
                     /locus_tag="AC16UZ_00595"
     CDS             complement(<1..992)
                     /gene="gatB"
                     /locus_tag="AC16UZ_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR6245202.1"
                     /translation="MGFRIPQGWEAVIGLEVHVQLATRSKIFSGASTRFGAEPNTQAC
                     PVDLGLPGVLPVLNAEAVRMAVTFGLAVGAEIRPYSVFDRKNYFYPDLPKGYQISQLP
                     HPIVGRGAITIEASGEAPRTIGITRAHLEEDAGKSIHDAYAGLTGIDLNRAGTPLLEV
                     VSEPELRTPEEAGAYFRKLHSLVRHLKICDGNLAEGSMRCDANVSVRPAGETALGQRT
                     EIKNVNSFRFLERAIEFEIERQIDVLEAGGAIEQQTLLYDPDSHETRPMRTKEYSEDY
                     RYFPDPDLLPVHVSEAFIESVRSTMPELPDARRARYAAEFQLGDYDAAWLTQDP"
     gene            complement(994..>2192)
                     /gene="gatA"
                     /locus_tag="AC16UZ_00600"
     CDS             complement(994..>2192)
                     /gene="gatA"
                     /locus_tag="AC16UZ_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR6245203.1"
                     /translation="CGSRMLDSWIAPYDATVVERLDDAGACLLGKANMDEFAMGSSNE
                     NSRFGPVRNPWDPGRVPGGSSGGSAAAVAAGLVPVASGTDTGGSIRQPSAFCGVSGIK
                     PTYGRVSRYGMVAFASSLDQGGFLGRSAEDLALSLAFCAGADPRDSTCSEHVDEWLDG
                     VAENGVGNLEGGLRIGLPREYFADLSTGSELLDAVAGFFESIGCSLEEVSLPHTSAAI
                     PAYYIIAGAEASTNLSRYDGVRFGYRCENPGSLEDLYRRSRSEGFGAEVKRRILTGTY
                     ALSVGYYDAYYVKAQRLRRLIQRDFLEAFETVDMILAPTAPGVAFPLGELTHDPVAMY
                     QQDIFTIPASLAGLPAMSLPCGFHQGLPIGVQLIGPHFAEKTLLQAGAAYQRETDWHT
                     RHPEAG"
BASE COUNT          359 a          707 c          767 g          359 t
ORIGIN      
        1 tccggatcct gggtgagcca ggccgcgtcg tagtcgccga gctgaaactc ggcggcgtag
       61 cgggcgcgtc gggcatcggg cagttccggc atggtcgagc gcaccgactc gatgaacgct
      121 tcgctgacgt gcacgggcag caggtcgggg tccggaaagt agcggtagtc ctcggaatat
      181 tccttggtgc gcattggcct ggtctcgtgg ctgtccggat cgtagagaag cgtctgctgc
      241 tcgatggcgc cgccggcttc gagaacgtcg atctgccgct cgatctcgaa ctcgatggcg
      301 cgctcgagaa aacggaaaga gttgacgttc ttgatctccg tgcgctggcc cagagctgtc
      361 tcgccggcgg ggcgcaccga gacgttggcg tcgcagcgca tgctgccttc ggccaggttg
      421 ccgtcacaga ttttcaggtg gcgcaccagg ctgtgcagct ttctgaagta tgcgcccgcc
      481 tcctccggcg tgcgcagctc gggttccgag accacctcga gaagcggggt gccggcccgg
      541 ttcagatcga tgccggtcaa accggcgtac gcgtcgtgaa tggacttacc ggcgtcctct
      601 tccaggtggg cccgcgtgat gccgatggtc cggggcgctt cgccggaggc ttcgatggtg
      661 atcgcgcccc ggccgacgat gggatgaggc agctggctga tctgatagcc cttgggaagg
      721 tccgggtaga agtagttctt ccggtcgaac accgagtagg gtcggatctc cgccccgacg
      781 gcgaggccga acgtcacggc catgcgcacg gcttccgcgt tcagaacggg aagcaccccg
      841 ggcaggccca gatccacagg gcaggcctgg gtgttcggct ccgcgccgaa ccgggtcgag
      901 gccccggaga atatcttgct tctcgtggcc agctggacgt gcacctcgag cccgatgacg
      961 gcctcccaac cctggggaat ccggaagccc atctcagccg gcctccggat gccgggtgtg
     1021 ccagtcggtc tcgcgctgat aggccgcgcc cgcctgcagg agggtctttt ccgcgaagtg
     1081 cggcccgatg agctgcacgc cgatgggaag cccctggtga aaaccgcacg gcagggacat
     1141 ggccggcagg ccggcaaggc tcgccggtat cgtgaagatg tcctgctggt acatggcgac
     1201 gggatcgtgg gtcagctcgc cgagcgggaa ggccacgccg ggggcggtgg gcgcgaggat
     1261 catgtccacg gtctcgaacg cctcgaggaa gtccctctgg atcagccgcc gcagccgctg
     1321 agccttcacg tagtaggcgt cgtagtagcc caccgacagc gcataggttc cggtgagaat
     1381 gcgtcgcttg acctcggcgc cgaacccctc ggagcgcgag cgcctgtata ggtcctccag
     1441 tgagcccgga ttctcgcagc gatagccgaa acgcacgccg tcgtagcgcg acaggttggt
     1501 ggacgcctcc gcgccggcaa tgatgtagta ggcgggaatc gctgcgctgg tatggggcag
     1561 cgagacttcc tcgagcgagc agccgatgct ttcgaagaag ccggccacag cgtccagcag
     1621 ctccgagccc gtggaaagat ccgcgaagta ttcccgcggc agcccgatcc gcagaccgcc
     1681 ttccagattt ccgacgccgt tctcggcgac cccgtcgagc cactcgtcaa cgtgctcgct
     1741 gcaggtggaa tcccgcgggt ccgcgccggc gcagaaggcc agtgacaggg caagatcctc
     1801 ggcgcttctg cccaggaaac ctccctggtc caggctcgac gcgaacgcca ccatgccgta
     1861 gcgggacacg cggccgtagg tgggcttgat gccggaaacg ccgcagaagg cggacggctg
     1921 acggatggag ccgccggtgt ccgtgccgga ggccacgggc acgaggcccg cggcgactgc
     1981 ggcggccgaa ccgccggagg atccgcccgg tactcggcct gggtcccagg gattgcgcac
     2041 cggcccgaag cggctgttct cgttggacga gcccatggcg aactcgtcca tgttggcctt
     2101 gccgagcaga caggcgccgg cgtcgtccag gcgctctacc acggtggcgt cgtagggggc
     2161 gatccagcta tccagcatcc gcgacccgca gc
//
LOCUS       DBNHOX010000030         3686 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_452532, whole genome shotgun sequence.
ACCESSION   DBNHOX010000030 DBNHOX010000000
VERSION     DBNHOX010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_452532"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="AC16UZ_00605"
     CDS             complement(<1..1167)
                     /locus_tag="AC16UZ_00605"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6245204.1"
                     /translation="MLRRVAASLRRPVASLCAILFVSGPALAAEIEEIVVTARGVEQS
                     VRDIPVAISVVSEERMANLNLKSLEDIAEVTPQLNIVRASSGSGTSISIRGIGSSPST
                     IGIEQSVSTIVDGVYYPQGRVINEAMFDVRQVAILKGPQALYFGKNATAGVISVETNN
                     PGDELEIIGRVGYEFEQERMEYEGIISSPLTETLGARLALRYTDMNEGYISNPVGTTT
                     YTTVDQATGTVTARENPPPDKDSWPGTESFYGRLTLAWDPSDQFGLNIKGSYADAEWV
                     SPTGSRELWNCPTLNGTPHRIQDGAPVPNDLAECKQDLKSSENAIPPEMAATNPRLGK
                     FGRNLGEDYTSYAFTVTGNWSLERFDVTGIFNYHDQETNWVGDFDGGGATAIMAS"
     gene            complement(1325..1852)
                     /locus_tag="AC16UZ_00610"
     CDS             complement(1325..1852)
                     /locus_tag="AC16UZ_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6245205.1"
                     /translation="MSDRVSSYRFFQEAYLVNDLDQAIRGWSDLYGAGPFRTVMHHKT
                     DRFHYRGTDQEADVSYAFGYLGDMQIQFIEQHDDTPSIYRDMYPAGEEGFHHVAILVH
                     DFEAEYERLVGKGFTDACRLFADGVDAAYFDTRSVNGCFTEIHGDPPRNLGRWAQMRR
                     AHELWRPGDDVYMTL"
     gene            1954..3054
                     /locus_tag="AC16UZ_00615"
     CDS             1954..3054
                     /locus_tag="AC16UZ_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6245206.1"
                     /translation="MAGTSVADSALRQWLDIQSIRNVLAIHSRGIDRADGDLLRSAYH
                     PDAEVAYGFFDGPAAELCEIMGNPSEQITMHRTSNMWIRLLDDDHAISESYAFVYSES
                     ADDAGYGQALIGGRYLDRHEKRGGEWRISHRTYVLEWNANWPGTGTVLPDYESPINRR
                     GSKTPDDLGTRLLNESQQILTSGDATMTIPEDLEAAVADALARQDIHDLIMAQARATD
                     RRDRALLESVFHEGATMDAGGLFDGPAPEYCDFILEMTAPLKKMYHSVANEWVRVEGD
                     SGVAESYVIAFTTAADEAGGDVDSLSGGRYLDRFERRDGVWKFTHRAYVMDWTMHQPT
                     TDQSDDPDSMYASLTNRGGHWPDDPVYGLWGK"
     gene            3058..>3686
                     /locus_tag="AC16UZ_00620"
     CDS             3058..>3686
                     /locus_tag="AC16UZ_00620"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6245207.1"
                     /translation="MSETSDRPAPGEARMPGPSYRDFILRDRAAPPAAFLEESYEFQG
                     DEDIPYSNYTSHDHAQAEFEKMWPRVWQWACREEHIPNPGDFHVYDVGDLSAIVVRTA
                     SGAVKAYFNACMHRGTQLKPPGMTGSSEDLRCPFHGWTWSLDGDLVDLPQAWDFPHVT
                     EDSHRLPEVKVGLWGGFVFVNFDPDAAPLEDYLGVLPRHFADFHIEDRY"
BASE COUNT          636 a         1165 c         1221 g          664 t
ORIGIN      
        1 cgaggccatg atggcggtgg cgccgccgcc gtcgaagtcc cccacccagt tggtttcctg
       61 gtcgtggtag ttgaagatgc cggtgacgtc gaagcgctcc aggctccagt tgccggtcac
      121 cgtgaacgcg taggacgtgt agtcctctcc cagattgcga ccgaacttgc ccagcctcgg
      181 gttggtggcc gccatctccg gcggtatcgc gttttccgag ctcttcagat cctgcttgca
      241 ctcggcaagg tcgttgggca cgggggcacc gtcttggatt cgatggggtg taccgttcag
      301 ggtcggacag ttccacagct cccggcttcc ggtgggagag acccactcgg cgtcggcgta
      361 ggagcccttg atgttcaggc cgaactgatc actcgggtcc caggccaggg tcagacgacc
      421 gtaaaagctc tcggtgccgg gccagctgtc cttgtccggg ggcgggttct cgcgcgctgt
      481 cacggttccg gtggcctggt cgacggtggt gtaggtggtc gtgccgacgg ggttgctgat
      541 gtaaccctcg ttcatgtcgg tatagcgcag tgccagcctc gcgcccaggg tctcggtgag
      601 cgggctggag atgatgcctt cgtactccat gcgctcctgc tcgaactcgt agcccacgcg
      661 accgatgatc tcgagctcgt cgccggggtt gttggtctcc acggagatga cgccggccgt
      721 ggcgttcttg ccgaagtaca gagcctgggg gcctttcagg atggccacct ggcggacgtc
      781 gaacatggcc tcgttgatga cgcgcccttg cgggtagtaa acgccgtcga cgatggtgga
      841 cacggactgc tcgatgccga tggtgctcgg cgaggagccg atgccgcgga tgctgatgct
      901 cgtgccgctg ccggagctgg cgcggacgat gttgagctgg ggcgtcacct cggcgatgtc
      961 ctccaggctc ttcagattga gattggccat gcgctcctcg ctgaccacgg aaatggccac
     1021 cgggatgtcc ctgacgctct gctcgacacc ccgggcggtg acgacgatct cctcgatttc
     1081 ggccgccaga gcggggccgg acacaaacaa gatggcgcaa agtgacgcca ccgggcgtcg
     1141 cagagacgcc gcaactcttc tcagcatttt aatctcccca agaacggatc ggattccgaa
     1201 cgagcggggt tttaacccga accgggtttc agcgcaaccg gccgttccgg tttcaggcgt
     1261 tcatgcgaac gtcgcccaca aaggcggggc cgtccggccg gttcgaaaac gccgccccgc
     1321 cgcgctacag cgtcatgtag acgtcgtcac ccggccgcca gagctcgtgg gcgcggcgca
     1381 tctgcgccca gcggccaaga ttgcggggcg ggtcgccatg aatctccgtg aagcagccgt
     1441 tgaccgaccg ggtatcgaag taggcggcgt ccacgccgtc ggcaaagagc cggcacgcgt
     1501 cggtgaaccc cttcccgacc aggcgttcgt actcggcctc gaagtcgtgg acgaggatgg
     1561 caacgtggtg aaagccttcc tcgccggccg ggtacatgtc gcggtagatg gagggggtgt
     1621 cgtcgtgctg ctcgatgaac tgaatctgca tgtcgcccag gtagccgaac gcgtaggaga
     1681 cgtcggcttc ctgatccgtg ccgcggtagt gaaagcggtc ggtcttgtgg tgcatgaccg
     1741 tccggaaggg gccggcgccg tacaggtcgc tccaacccct gatggcctga tccagatcgt
     1801 tgacgaggta ggcctcctga aagaagcggt agctgctcac gcggtcggac atgtggtttc
     1861 cccttccctc ggaccctggc ttcctgcgac attagcgcac gccgggctgt taggatagat
     1921 ttggcgattg gatcaggccc cgggggaggg accatggccg gaacaagcgt cgcagactcg
     1981 gctctgcgcc agtggctcga cattcaatcc atccgcaacg tgctggcgat ccacagccgc
     2041 ggcatcgatc gcgccgacgg cgatctgctg cgttccgcct accatccgga cgccgaggtc
     2101 gcctacgggt tcttcgacgg cccggccgcc gagctctgcg agatcatggg caatcccagc
     2161 gagcagatca ccatgcaccg caccagcaac atgtggatcc ggctgctgga cgacgatcac
     2221 gccatctccg agagctacgc gttcgtctac tcggaatcgg ccgacgatgc cggctacggg
     2281 caggcgctca tcggcggccg ttatctcgac cggcacgaga agcggggcgg ggagtggcgg
     2341 atcagccacc ggacctacgt gctggagtgg aacgccaact ggccgggcac cggtaccgtg
     2401 ctgcccgact acgaaagccc catcaaccgc cgcggcagca agacgcccga cgatctcggc
     2461 acccgtctgc tcaacgagtc acagcaaatt ctcacttccg gagatgccac catgaccatt
     2521 cccgaggacc tggaagccgc cgttgcggat gcgctcgcaa gacaggacat tcacgatctg
     2581 atcatggccc aggcgcgagc caccgaccgc cgggaccggg ctctgctcga gtcggttttc
     2641 cacgaagggg cgacgatgga tgcgggcggg ctgttcgacg gcccggcccc cgagtattgc
     2701 gatttcattc tcgagatgac ggcgcccctg aagaagatgt accactcggt tgccaacgag
     2761 tgggttcggg tggaaggcga cagcggcgtg gcggagagct acgtgatcgc cttcacgacg
     2821 gccgcggacg aggccggcgg cgacgtggac agcctctcgg gtgggcgtta tctggaccgt
     2881 ttcgagcgcc gcgacggggt ctggaaattc acgcatcgag cctacgtgat ggactggacc
     2941 atgcatcagc ccaccacgga ccagagcgac gatccggaca gcatgtacgc gagcttaacc
     3001 aatcgcggcg gtcactggcc ggacgacccg gtctacggcc tgtgggggaa gtgaaagatg
     3061 agtgagacct ctgacagacc cgctcccggc gaagcgcgca tgccgggacc cagctaccgg
     3121 gacttcatcc tgcgcgatcg cgcggccccg cccgctgcct ttctcgagga gtcatacgag
     3181 ttccagggcg acgaggacat tccttacagc aattacacct cccacgatca tgcgcaggcc
     3241 gagttcgaga agatgtggcc gcgggtatgg cagtgggcgt gccgggagga gcacatcccc
     3301 aatcccggcg actttcacgt ctacgacgtc ggtgatctct cggccatagt ggtgcgtact
     3361 gcaagcggcg cggtcaaagc ctatttcaac gcctgcatgc accgcggcac ccagctgaag
     3421 ccgccgggca tgaccggatc gtccgaggat ctgcgctgcc cgttccacgg ctggacgtgg
     3481 tcgctggacg gggatctggt ggacctgcct caggcgtggg acttccccca cgtcacggag
     3541 gacagccatc ggctgccgga ggtcaaggtc ggcctgtggg gcgggttcgt tttcgtcaac
     3601 ttcgaccccg acgcagcgcc gctggaggac tatcttggcg tgctgccgcg gcacttcgcc
     3661 gacttccaca tcgaggaccg ctacat
//
LOCUS       DBNHOX010000031         1618 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_838151, whole genome shotgun sequence.
ACCESSION   DBNHOX010000031 DBNHOX010000000
VERSION     DBNHOX010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_838151"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="AC16UZ_00625"
     CDS             <1..1384
                     /locus_tag="AC16UZ_00625"
                     /inference="COORDINATES: protein motif:HMM:NF046195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR6245208.1"
                     /translation="SCHESLDSWAPLFQAPGGASFHLAFDESEDCGQCHGVLSTAKPY
                     VADFHNGILTERSGIIWNGEDTSVEEGERFVWKIDGVVDNGATLAITWSASYDTVGVN
                     PCNATVGPGAPVFHRVSGGSGGTLNIRYTWLQGEDPILGTAGNPGQAANVNLSTSNTT
                     CAGNVATTTIPVTRVAGATKGRIALMGKPGVVSVADPTTFMRVRVPSPTFDFAVGTGT
                     EVNRRAIVDTKDKCLKCHVGSLYQHGGDRVDNIDACMVCHNSASNDKFVREDMGVDAS
                     EAYDGRVGQTFEMKSMLHKIHTAGLGPRSDGLEIGGKPIAIYRGRGIYYWGLDEPANW
                     PDPVADACVRTNGTAGQWVYGADRTVDGNCQPHNFHTPTFPRRANDCGACHVAFDQIP
                     DATKAMATTLDPGEEPWTNQLDDVLQGAGAASCTTCHSSSDARAHANPMGWVPQVFPN
                     GRQDIIDEAN"
BASE COUNT          293 a          525 c          544 g          256 t
ORIGIN      
        1 cagctgccac gagtccctgg acagctgggc gccgttgttc caggcgccgg gcggcgcctc
       61 gttccacctc gccttcgacg agagtgaaga ctgcggccag tgccacgggg tcctgtcgac
      121 ggcgaagccg tacgtggcgg acttccacaa cggcatcctg acggagcgta gtggcatcat
      181 ctggaacggc gaggacacgt cggttgagga gggcgagagg ttcgtctgga agatcgacgg
      241 agtcgtcgac aacggcgcga cgctggcgat cacctggagc gcttcctacg acaccgtcgg
      301 cgtgaacccc tgcaatgcca ccgttggccc gggtgccccg gtgttccaca gggtgagcgg
      361 tggcagcggc ggcacgctga acatccggta cacctggctg cagggcgagg acccgatcct
      421 tgggaccgcg ggtaatccgg gccaggccgc caacgtgaat ctgtccacca gcaacacgac
      481 ctgtgccggc aacgtggcga ccaccacgat cccggtgacc cgtgtcgcgg gggccacgaa
      541 gggcaggatc gccctgatgg gcaagccggg cgtggtcagc gtcgcggacc cgacgacctt
      601 catgcgggtc cgtgtcccgt ccccgacttt cgatttcgcc gtcggcacgg gcaccgaggt
      661 gaatcgtcgc gcgatcgtcg acaccaagga caagtgcctg aagtgccacg tcggctcgct
      721 gtaccagcac ggtggcgacc gggtggacaa catcgacgcg tgcatggtct gccacaactc
      781 ggcgtcgaac gacaagtttg tccgtgagga catgggcgtc gatgcctccg aggcctacga
      841 cggccgggtg ggccagacgt tcgagatgaa gtccatgctg cacaagatcc acacggctgg
      901 cttgggtccg cgttcggacg ggctggagat cggcggcaag ccgattgcca tctaccgcgg
      961 ccgcggcatc tactactggg ggctggacga gcccgccaac tggccggatc cggtcgctga
     1021 cgcgtgcgtc cgcaccaacg gtaccgcggg acagtgggtc tacggtgcgg atcggacggt
     1081 ggatggaaat tgccagccgc acaacttcca cacgccgacc ttcccgcggc gggcgaacga
     1141 ctgcggtgcc tgccacgtgg cctttgacca gatacctgac gcgaccaagg cgatggccac
     1201 cacgctcgat ccgggcgagg agccgtggac gaaccagctc gacgacgtcc tgcagggcgc
     1261 cggcgcggct tcgtgcacga cctgccacag ttcgagcgat gcaagggccc atgcgaaccc
     1321 gatgggctgg gtcccgcagg tcttcccgaa tggccggcag gacatcatcg acgaggccaa
     1381 ctagctgatc tccggtgaac gggacgggcc atcgggcctg ctccctcacc gggaagcaga
     1441 cactccgagg cggaccccca gggtccgcct cttttctttt tcagttcccg gaaagaaagc
     1501 gccgcgcagg gagcgcggcg cctcgttcag ccagcaggcg agtggtgaat ttgtcttcga
     1561 aacacgaaaa ggctgacgac cgcctgctct ttgccgcgat ttcttcccaa aggatcat
//
LOCUS       DBNHOX010000032         6020 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_838167, whole genome shotgun sequence.
ACCESSION   DBNHOX010000032 DBNHOX010000000
VERSION     DBNHOX010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_838167"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="AC16UZ_00630"
     CDS             complement(<1..530)
                     /locus_tag="AC16UZ_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR6245209.1"
                     /translation="MLRTIRDFFFERGVIEVQSACLGAHTVTDPAVDSLAVPGIGYLQ
                     TSPEYQLKRLLAAGAPSLYQLGPVFRGGEAGRLHNPEFTMLEWYRLGYTDAELMAEVA
                     ELIDSVLGAADYERLSYERVVALGGGAESGAERAELDLAFADGIAALAGRRVFVVDYP
                     ADQAILARLRPGRPNVA"
     gene            complement(593..1159)
                     /gene="efp"
                     /locus_tag="AC16UZ_00635"
     CDS             complement(593..1159)
                     /gene="efp"
                     /locus_tag="AC16UZ_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR6245210.1"
                     /translation="MATVSTNEFRSGLKVLLEGEPCSILENEFVKPGKGQAFNRVKFR
                     NLITGRVWERTFKSGDSLEAADVMELDMEYLYSDGDFWHFMRTDGSYEQSAAPPSAVG
                     DAKDWIKEQEVCQVTLWNDEPISVTPPNFVELQVTETDPGLKGDTAQGGTKPATLSTG
                     ATIKVPLFINVGDVLRVDTRKGEYVSRA"
     gene            1404..3521
                     /locus_tag="AC16UZ_00640"
     CDS             1404..3521
                     /locus_tag="AC16UZ_00640"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR6245211.1"
                     /translation="MAKKDMTAQHNPSAPVRLLIAENSENAAQEFDSLLRDAGIPTRS
                     EIIDLPMAVDAMHEADLLLANSSLPELEQIIPRLSDQAPNVPIVLVNYDDATITTTRG
                     MQLGAADVVPKSEPEHLVLVVKRELEHVLQYQSLSQTRRALQETEQRCQLLLRSSKAA
                     IAYVHDGMHIYANEGYLKLFGFEDVEDLIGLPHMDLLDADSAVALKVAMKQFQRDGEE
                     KEIEFVGQNTAEETIAGTMTLAAAEYEGEHCMQVTVRTGRATDAPATAQAAADDASTA
                     NGEGSAPADVRLHAFFDAVAGNPRTRSEDAFSAIFVAEIDDYAEVQRSHGLAGALEVC
                     RHVERCLGELIASDALHRLADHQFAFAVTADNEDEVRAEADQLRGTIEASLLEVREKT
                     VRPTITLAGMEIATEEDQGVRERLEDCLNEAYDVLRTSMEQNEGNCVELKSQGAGDEP
                     ESESARLLRLINEAIDDQRFVLLFQPMISLHGDSDEHYEVLLRMLDDEGKQMLPGAFL
                     RTAIENNLAGRIDRWVILQAIKSLSAHRAKGNLTRLTINLTSNSVNDPEFLEWLGVAF
                     KAVRLPTDAVVLQITEQDANTYVRQTREFFEQLKQMHCVASLSRFGRLGNSFELLEQI
                     PAEYVKVDGSLIEKIASSSEAREALTEMIHGLQGAGKLTVVPMVESANILSTLWQAGA
                     NYVAGHYLQEPSRSMDYDFSADD"
     gene            complement(3556..4794)
                     /gene="serB"
                     /locus_tag="AC16UZ_00645"
     CDS             complement(3556..4794)
                     /gene="serB"
                     /locus_tag="AC16UZ_00645"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HIR6245212.1"
                     /translation="MSSEIVLVNVSGEDQPGLMAMLTSTLARYQVRVLDVGQAVIHDE
                     LNLGMLLQIISDGNGVEAAEVLSAIEANVLDQGMTARFTPISAGDYADWVKSQGKPRY
                     IVTLLTAGATGEQLAAVTSIVRHHGLNIDAIRRLSGRLPLTSDAGGQRICIEMSLRGV
                     LADAGALKAGLLEAAERLAFDFSVQQDTVYRRNRRLVAFDMDSTLIDAEVIDELAAAH
                     GVGEQVARITERAMRGELKFADSFRERAMLLKGLSEDALREVADGVALNDGARRLIRT
                     LKHFGYKTAIISGGFQYVGERLRDELGIDYVYANSLEIRDGLMSGEVEGEIIDAGRKA
                     ELLKEIARREGISGQQTIAIGDGANDLPMLNAAGLGVAYHAKPMVKETASHAISTFGL
                     DSVLYLMGFSDRDIDEALTG"
     gene            4820..5941
                     /locus_tag="AC16UZ_00650"
     CDS             4820..5941
                     /locus_tag="AC16UZ_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245213.1"
                     /translation="MKLTSWLGLVKPPPLSLVGACLGSLVSVAVMLSYWALNDPDQLR
                     HDAYGRSMSTGLAQLAIEPLLTHDRITLGVIANQAADIPGVSGVEIVTVNEQALASSG
                     SLSNARVYQSPVTFNDQIMGAVRVALSEPPSHLGDAVAVAAVTLIAAPFLALALLSIR
                     LQPPLPRQPARIEVELHTPRERHFLVAVNLYDQLGMEPTQRVAELNYARIAAGKVAGL
                     YGGDVENLPGTGLLLDFNDGGDADRPWQVLSAAAVLSELLDEEAGDYRVAIHVGDAPV
                     GHRVLLDEQAVGDAALLSALAKPATLAASSVFSACLDRGGELVERTVIEHPLLEELAT
                     VGPDSVLITALSAPQQDLVQHQAAHLAGHRPATASESTF"
     gene            complement(5905..>6020)
                     /locus_tag="AC16UZ_00655"
     CDS             complement(5905..>6020)
                     /locus_tag="AC16UZ_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012316496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245214.1"
                     /translation="LRMKHRDLENYVGERARRGRKLPRGFQKVDSLAVAGR"
BASE COUNT         1091 a         2006 c         1894 g         1029 t
ORIGIN      
        1 gcaacattcg gccgcccggg gcgcagccgc gcgaggattg cctgatctgc cggatagtcc
       61 accacgaaca cccggcggcc ggcgagggcg gcgatcccat ccgcaaacgc gaggtccagc
      121 tctgcacgct ccgccccact ctcggcgcca ccgcccaggg caacgacccg ttcgtagctg
      181 agcctctcgt agtcggcggc ccccagcacc gagtcgatca gctcggcaac ctcggccatg
      241 agctccgcgt cggtatagcc aagccggtac cactccagca ttgtgaattc ggggttgtgc
      301 aaccgtcccg cctccccgcc gcgaaatacg ggcccgagct ggtaaaggga aggtgccccg
      361 gctgcgagca ggcgcttgag ctgatactcg ggagatgtct gcagatagcc gatcccgggc
      421 accgccagcg agtcgacggc cgggtccgtg acggtgtgcg cgccgaggca ggcagactgc
      481 acctcaatga ccccgcgctc gaaaaagaaa tcccggatcg tccgcagcat ggcggcgcgc
      541 gcgccgagcg cttcaatcga gcacgccggg cgccaggcgg ggggtggcgc gttcaagcgc
      601 ggctgacgta ctcgcctttg cgggtatcga cgcgcagcac gtctccaacg ttgatgaaca
      661 agggcacctt gatggtcgcg ccggtagaca gcgtggccgg cttggtgccg ccctgggcgg
      721 tatcaccctt gagccccggg tcggtttcgg tcacctgaag ctcgacgaag ttcggcggcg
      781 tgacggagat cggctcgtcg ttccagagcg tcacctggca gacctcctgc tccttgatcc
      841 agtccttggc gtcacccacc gcgctcggcg gcgccgccga ctgctcgtag ctgccgtcgg
      901 tgcgcatgaa gtgccagaag tcgccgtccg agtacaggta ctccatgtcg agctccatga
      961 cgtccgcggc ttccaggctg tcgcccgact tgaacgtccg ctcccaaacc cgaccggtga
     1021 tcagattgcg aaacttgacc cgattgaagg cctggccctt ccccggcttc acgaactcgt
     1081 tctcgaggat cgagcagggc tcgccctcca gaaggacctt cagtcccgag cggaactcgt
     1141 tggtagaaac agtcgccatg tgcagcggac cagtgtggaa tcggcggcat catatccgct
     1201 tggccgtcga ggggatagcc ggcttcggag cggcgccggg gcttccggac ttgctcggat
     1261 tcacgctctc tggtcaaagc gcgacccggt tcacatttcg tctgttggat ggttacttgg
     1321 attggccacg tttcggccgc accatgagtt cctccgcgga cgtccaatgc ccgacgtccg
     1381 ggtgattcga aaaaagggaa aggttggcta aaaaggatat gacggctcag cacaatccca
     1441 gcgcgcccgt gcgcctgcta attgcggaga attccgagaa tgcggcgcag gagttcgact
     1501 cgctcctgcg cgacgcaggc attcccaccc ggtcggagat catcgatctt cccatggccg
     1561 tcgacgccat gcacgaagcg gatctgctgc tggcgaactc ttctctgccc gagctagagc
     1621 agatcatccc gcggctcagc gaccaggcgc ccaacgttcc gatcgttctc gtcaattacg
     1681 atgacgcgac gatcacgacc acgcgcggca tgcagctcgg cgccgccgac gtggtcccga
     1741 aatccgagcc ggaacacctc gtcctggtgg tcaagcggga gctcgagcac gttctccagt
     1801 accagagcct gagccagaca cgccgggcac tccaggaaac cgagcagcgc tgtcagctgc
     1861 tgctcagaag ctccaaagcc gccatcgcct acgtccacga cggcatgcac atctacgcca
     1921 acgagggtta cctgaagctc ttcggctttg aagacgtgga ggacctcatc gggctgccgc
     1981 acatggatct gctcgacgcc gacagcgccg tggccttgaa ggtcgcgatg aagcagttcc
     2041 agcgcgacgg cgaggaaaaa gagatcgagt tcgtcggtca gaacacggcc gaagagacca
     2101 ttgcgggcac catgaccctc gccgccgccg agtacgaggg cgagcactgc atgcaggtca
     2161 ccgtgcgaac cggccgggca accgacgccc ctgcgaccgc gcaggccgca gccgacgatg
     2221 cttcgactgc caacggcgaa ggcagcgcgc cagccgatgt gaggcttcac gccttcttcg
     2281 acgcggtggc cggcaacccc aggacccgct ccgaggacgc attttccgcg attttcgtcg
     2341 ccgagatcga cgattacgcc gaggtgcagc gatctcacgg tctggctgga gcgctcgagg
     2401 tctgccgtca tgtcgagcgg tgtctcggcg agctgatcgc atcggacgcg ctgcatcgac
     2461 tggcggatca tcagttcgcc tttgcggtaa ccgccgacaa cgaggacgag gtccgagcag
     2521 aggccgatca gcttcgcggg accatcgagg catcgctgct cgaggtcagg gagaagaccg
     2581 tacggcccac gattaccctc gccggcatgg agatcgccac ggaggaagat cagggcgtcc
     2641 gtgagcggtt ggaggactgt ctgaacgagg cctacgatgt cctgcgcaca tccatggaac
     2701 agaacgaagg aaactgcgtc gagctgaagt cccagggagc aggcgacgag ccggagtccg
     2761 aatcagcaag gctgctgcgc ctgatcaacg aggccatcga cgaccagcga ttcgttctgc
     2821 tgttccagcc gatgatcagc ctgcacggcg actccgacga gcactacgaa gtgctgctgc
     2881 gcatgcttga cgacgaaggc aaacagatgc tcccgggcgc tttcctgcgc acggccatcg
     2941 agaacaacct cgccggacgg atcgacagat gggtcattct ccaggcgatc aagtcgctgt
     3001 ctgcgcaccg tgccaagggc aacctgaccc ggctgaccat caacctgacc agcaacagcg
     3061 tcaacgaccc ggagttcctg gagtggcttg gcgtcgcctt caaggccgtt cggctgccta
     3121 ccgatgcggt ggtcctgcag atcacggagc aggacgccaa cacctacgtt cgccagaccc
     3181 gcgagttctt cgagcagttg aagcagatgc actgcgtagc ctcgctgtcg cgatttgggc
     3241 gcctcggcaa ctctttcgaa ctgctcgaac aaatcccggc ggaatacgtc aaggtcgacg
     3301 gttcgctgat cgagaagatc gccagcagct cggaggcgcg tgaggcgctg accgagatga
     3361 ttcacggcct ccagggcgcc ggcaagctca cggtggtccc catggtggaa agcgccaaca
     3421 tcctgtcgac cctatggcag gccggtgcca actacgtggc cgggcactac ctgcaggagc
     3481 cctccagaag tatggattac gacttcagcg ccgacgactg atccccgctg accggatatc
     3541 gccggggcgg cggcttcagc ccgtaagcgc ttcgtcgatg tcgcggtcgc tgaagcccat
     3601 caggtacaga acgctgtcca ggccgaaggt ggagatggcg tggctagccg tctccttgac
     3661 catgggtttc gcgtggtatg ccaccccaag accggcggca ttgagcatgg gcaggtcatt
     3721 cgcgccgtcg ccgatggcga tggtctgctg gccggatatc ccctcacggc gggcgatctc
     3781 cttcaagagc tcggccttgc ggccggcgtc tatgatctcc ccttcgacct cgcccgacat
     3841 cagcccgtcg cgaatctcca gcgagttggc ataaacgtaa tcgatgccca gctcgtcacg
     3901 cagccgctca ccaacgtact gaaagccgcc tgagatgatg gccgtcttgt agccgaaatg
     3961 cttgagcgtg cggattaggc gccgggcacc gtcgttcagc gcaacgccgt ctgccacctc
     4021 acgcagggcg tcctcggaaa gacccttgag cagcatggcc cgctctcgaa agctgtcggc
     4081 gaacttcagc tcaccgcgca tggctcgctc ggtgatccga gctacctgct cgccaacccc
     4141 gtgcgcggcc gccagctcgt cgatgacctc ggcatcgatg agcgtagagt ccatgtcgaa
     4201 cgccaccagc cgccggttgc gccggtacac ggtgtcctgc tgcacgctga aatcgaacgc
     4261 caggcgctcg gcggcttcga gaaggcccgc tttcagggcg ccggcatcgg caaggacgcc
     4321 gcgcaggctc atctcgatgc agatccgctg accaccggcg tcggacgtca agggcaagcg
     4381 ccccgacagc cgtcgtatgg cgtcgatgtt gaggccgtgg tgacgaacga tggaggtcac
     4441 cgcggcgagc tgctcgccgg tggcgccggc cgtgaggagg gtgacgatat agcggggctt
     4501 gccctggctc tttacccagt cggcgtagtc gcccgccgag atgggagtga agcgcgccgt
     4561 catgccctga tcgagaacgt tcgcctcgat ggcggacagc acctcggcgg cctccacgcc
     4621 gttaccgtca ctgatgatct gcagcaacat gccgaggttg agctcgtcgt ggatcaccgc
     4681 ctgccccacg tcgagcaccc gcacttgata acgtgccaga gtggatgtca acatggccat
     4741 caggccaggc tggtcctctc cggacacgtt caccaataca atctcgctgc tcacatcgcc
     4801 gcgcggaata ggaagtagtt tgaagctaac gtcttggctg gggctcgtca aaccaccgcc
     4861 gctttcgctg gtcggcgctt gcctgggctc gctggtttcc gtggccgtga tgctgtccta
     4921 ctgggctctc aacgatcccg atcagctccg tcacgacgcc tacggcagga gcatgtccac
     4981 agggctcgcc cagctggcca tcgagccgct gctgacccac gatcgcatca cgctgggcgt
     5041 gatcgccaat caggcggcgg acattcccgg cgtcagcggg gtcgaaatcg taacggtcaa
     5101 cgagcaggcg ctcgcctcga gcggctccct ttcgaacgcg cgcgtctacc agagcccggt
     5161 gaccttcaac gaccagatca tgggcgccgt gcgggtcgcc ctgagcgagc ctccaagcca
     5221 cctcggggat gccgtggcgg tggccgctgt caccttgatt gctgcgccct tcctggcgct
     5281 ggccctgctc agcatccggc ttcaaccgcc cctgccgagg cagcccgcgc gtatagaggt
     5341 ggagcttcac acgccgcggg aaaggcactt cctcgtcgcc gtgaacctgt acgaccagct
     5401 cggcatggag cccacgcaac gcgtcgccga gctgaactac gcgcgcatcg ccgcgggcaa
     5461 ggtcgccggc ctgtacggcg gcgatgtgga aaatctgccc ggtaccggat tgctgctgga
     5521 cttcaacgac gggggcgatg cggaccgtcc ctggcaggtg ctgagcgcag cggcggtgct
     5581 ctcggagctt ctcgacgagg aggcgggtga ctatcgagtg gccattcacg tcggcgacgc
     5641 gccggtcggc catcgcgtgc tgttggacga gcaggcggtc ggcgatgccg cgctcctgtc
     5701 cgcgctcgcc aagccggcca ccctggccgc gagcagcgtt ttctccgcct gcctcgaccg
     5761 cggcggcgag ctggtggagc gcacggtcat cgagcacccc ctgctcgagg agctggcaac
     5821 ggtgggtccc gactcggtcc tgattacagc gctgtcagcc ccgcagcagg atctggtgca
     5881 gcaccaggcc gcccacctcg ccggtcaccg tcccgcaact gccagcgagt ctactttctg
     5941 aaatccgcgc ggaagcttgc gcccgcgccg tgccctctca ccaacgtagt tctcgagatc
     6001 cctgtgcttc atgcggagat
//
LOCUS       DBNHOX010000033         2393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1417069, whole genome shotgun sequence.
ACCESSION   DBNHOX010000033 DBNHOX010000000
VERSION     DBNHOX010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1417069"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..866)
                     /locus_tag="AC16UZ_00660"
     CDS             complement(114..866)
                     /locus_tag="AC16UZ_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245215.1"
                     /translation="MLTGNADLAELAAGAPTIAAFPTEPVQLEDVECFQLTAEMRSSA
                     RESVLPAGLHPTIPAALSVQAWKVGASPWGAFAVCFCRVSCRSGVRARGFSTAAVVQG
                     DAALSALRDAFGFPARPGAVNLGRHYDGVDLTVSDESGTTLEVAALDPDPLGLEDVQY
                     TGTMNLAHTPQGLRLVQVESGHSATRVERLSARILHFDGASWGNERLAPYRVIAASVA
                     LEDIVLPPVRFVCKPDEPAFTGTEPVGEPSGK"
     gene            complement(866..2014)
                     /locus_tag="AC16UZ_00665"
     CDS             complement(866..2014)
                     /locus_tag="AC16UZ_00665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6245216.1"
                     /translation="MKFGILFELSTPRPLTREHEWAVYHNALEQCRLADELGFDHVWA
                     VEHHFLEEYSHCSAPEVFLTAVAAQTRRVRVGHGAVVCVPQMNHPVRVAERAAALDIV
                     SNGRLEFGTARSSTWTELGGFEVNPDDTKKTWDEFVRAIPKMWMQEEFEWEGKSFSMP
                     ARNVLPKPLQDPHPPMWVTVTSPGTEIDAAERGLGCLGVSSAGFEEQERRTQAYHRLV
                     SQCEPVGGVINDQVHTMNFLYCHEDYRKARDVGVPMVTAFNVANAHLYWTREAYPTRA
                     YQTLGNAGSAMRAKKPAADDPSVAKGLPEGLGVGDPDHLIRTIKTWESIGVTGINFLL
                     NAMEMGPQEEVLESMRLFAREVMPHFKEPDERARPVWSPAGLPQVAAG"
     gene            complement(2064..>2393)
                     /locus_tag="AC16UZ_00670"
     CDS             complement(2064..>2393)
                     /locus_tag="AC16UZ_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6245217.1"
                     /translation="RGYLARSREAQHDTLRAGSDEFARMLYQRESDLDIIDRNAEVAA
                     GLGVKPAQTAIAWMLGKPGIVAPIIGASRPEHLADAAAAVELTLSEQDVSRLEALYEP
                     HAVSGHA"
BASE COUNT          367 a          795 c          827 g          404 t
ORIGIN      
        1 agtagaagac cccgcgacgg gcgctgaccc gcagccaggc gacgaggatt cactcgctgc
       61 gcaagatggg gcggtcccgc gcgcgcgcga agccggctag cctcctttca agcttacttt
      121 cctgacggct cgcctaccgg ctcggtgccg gtgaacgccg gttcgtccgg cttgcacacg
      181 aaccgcaccg gcggcagcac gatgtcctcc aacgccacgg atgcggcaat cacccggtac
      241 ggtgccaggc gttcgttgcc ccaggaggcg ccgtcaaagt ggaggattcg cgcgctcagg
      301 cgctccaccc gtgtggccga gtgcccggac tccacctgaa cgagccgcag gccttggggc
      361 gtgtgggcca ggttcatggt tcccgtgtat tgaacgtctt ccaggcccag aggatcgggg
      421 tccagggcgg cgacctcgag cgtcgtgccg ctttcgtcgc tgacggtgag atccacgccg
      481 tcgtagtgtc gacccaggtt caccgcgccg gggcgggccg gaaacccgaa ggcgtcccgc
      541 agcgcgctca gtgcggcgtc tccttgaacc accgcagcgg tggaaaaacc ccgggcgcgc
      601 acgccgctgc ggcagctgac ccggcagaag cagacggcga acgctcccca cgggcttgcg
      661 cccaccttcc atgcctgcac ggaaagcgcc gccggaatgg tggggtgcag acccgcgggc
      721 agcacggact cccgggcgga cgagcgcatc tcggcggtga gctggaaaca ctcgacgtcc
      781 tccagctgca ccggctcggt gggaaatgcg gcgatggtcg gggctcccgc cgccagctcg
      841 gccaggtcgg cgttgccggt cagcatcagc cggccgccac ctgcgggagc ccggcgggtg
      901 accagacggg ccgcgcccgc tcgtcgggtt ccttgaagtg cggcatgacc tcgcgcgcga
      961 agagccgcat gctttccagg acctcctcct gaggccccat ctccatggcg ttgagcagga
     1021 agttgatgcc cgtcacgccg atggactccc acgtcttgat ggtgcgaatc agatggtcgg
     1081 gatcgcccac gccgagccct tccggcaagc ccttggccac gctgggatcg tcggccgccg
     1141 gtttcttcgc ccgcatggcg gatccggcat tgcccagcgt ctggtaagcg cgggtcgggt
     1201 aagcctcccg ggtccagtag aggtgcgcgt tggcgacgtt gaacgccgtc accatgggca
     1261 cgccgacgtc ccgggccttg cggtagtctt cgtggcagta caggaagttc atggtgtgga
     1321 cctgatcgtt gatcacgcct cccaccggtt cgcattggct caccaggcgg tgataggcct
     1381 gggtgcggcg ctcctgctcc tcgaatccgg ccgacgatac tccaagacag ccgaggccgc
     1441 gctcggccgc gtcgatctcc gtgccggggc tggtcaccgt gacccacatg ggcggatggg
     1501 gatcctgcag cggcttgggc aggacgttgc gggcgggcat ggagaacgat ttgccctccc
     1561 actcgaactc ctcctgcatc cacattttcg ggatggcgcg gacgaactcg tcccaggtct
     1621 tcttggtgtc gtcggggttg acctcgaagc cgcccagctc cgtccaggtg gaggagcgtg
     1681 ccgtgccgaa ctcgagcctg ccgttggaga cgatgtcgag ggcggccgcc cgctcggcaa
     1741 ccctgacggg gtggttcatc tggggaacgc acaccaccgc gccgtggccg acgcgaacgc
     1801 ggcgggtctg ggcggcgacg gcggtgagaa agacttcggg cgcggagcag tgcgagtact
     1861 cctccagaaa gtggtgctcc acggcccaga catggtcgaa gccgagctcg tcggccagcc
     1921 ggcactgctc cagggcgttg tggtagacgg cccattcatg ctcccgggta agaggccgcg
     1981 gcgtggacag ctcgaagagg atgccgaact tcatggcttg gatgatcctt tcgtcaagtg
     2041 gtgtaaccga agcagaactg cctttacgcg tgtccggaca cggcgtgagg ctcgtagagc
     2101 gcttcgagcc gggacacgtc ttgctcggaa agcgtcagct ctaccgcggc ggcggcgtcc
     2161 gccaggtgct cgggtctgga cgcgccgatg atgggcgcga cgattcctgg cttgcccagc
     2221 atccacgcga tggccgtctg cgcgggcttc acgcccaggc cggcggccac ctctgcgttg
     2281 cggtcgatga tgtcgaggtc gctttcgcgc tggtagagca ttcttgcgaa ctcgtccgac
     2341 cctgcgcgca gggtatcgtg ctgagcttcc ctgctgcggg ccaggtagcc ccg
//
LOCUS       DBNHOX010000034         3120 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_67283, whole genome shotgun sequence.
ACCESSION   DBNHOX010000034 DBNHOX010000000
VERSION     DBNHOX010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_67283"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="AC16UZ_00675"
     CDS             <1..758
                     /locus_tag="AC16UZ_00675"
                     /inference="COORDINATES: protein motif:HMM:NF017591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HIR6245218.1"
                     /translation="GTWFSCEEVRRGRVYECDPLGLRQPVALPALGRFQHEAAVVDPL
                     TGTVYMSEDEPDGRLYRFRPTLPRHLERPDLSAGVLEVASLAPDGSGSVVWLRVPDPE
                     AGRKPTRWQVPESTGFDGGEGMSYCDGVVYLATKGDNRVWALDLGEDRLEVIYDQATA
                     ATPILGGVDNVETNASGDVLVAEDGDDMQIVVLPREGEPVALLQVTRQGGSEITGLAF
                     TPDMSRLYFSSQRGFSGRVTGGITYEVTGPFVG"
     gene            776..1849
                     /locus_tag="AC16UZ_00680"
     CDS             776..1849
                     /locus_tag="AC16UZ_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA nucleotidyltransferase/poly(A)
                     polymerase-related protein"
                     /protein_id="HIR6245219.1"
                     /translation="MDVYLVGGAVRDELLGRPVRERDWVVVGASPEAMLKRGFRQVGR
                     DFPVFLHPDTSEEYALARTERKTGPGHKGFECHAGPDVTLEQDLERRDLTVNAIARAS
                     DGSLIDPFGGRSDLEAKVLRHVSVAFSEDPLRVFRVARFAAQLDDFTVAEETRALMAE
                     LAGSGALAELSAERVWQELVKALGSKAPLRFFTVLASVGGLSTWFAELAEVEITFPEA
                     IKDALGRFAALGWHLAPEAVDTLCERLRAPRRHRRAMGHIASHGVVLAAWETAEAGSL
                     CAALKSVGAFKAGDDPEMAVRVSAACAGRNPEPLLELAASIRQEVTTASVGGSLTGAA
                     LGEAIDQARTQAIRQAQRSSVGR"
     gene            complement(1901..2254)
                     /gene="folB"
                     /locus_tag="AC16UZ_00685"
     CDS             complement(1901..2254)
                     /gene="folB"
                     /locus_tag="AC16UZ_00685"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF014233.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR6245220.1"
                     /translation="MDSVFVRNLTVSTIVGVYPEERTRPQRVIVSFELFADTRAAAQS
                     DDLEDALDYGEAASRVAEFVRGSRFELIEALADGIAALLLEGYPAARVVVEVLKPGAV
                     TDAETVGVKIERSRA"
     gene            complement(2258..>3120)
                     /gene="tsaD"
                     /locus_tag="AC16UZ_00690"
     CDS             complement(2258..>3120)
                     /gene="tsaD"
                     /locus_tag="AC16UZ_00690"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR6245221.1"
                     /translation="LVPLVRKTLDDSGRSLADVDGVAYTSGPGLMGALLVGAGFARSL
                     AWSLGKPAVAVHHMEAHLLAPLMNPDTAPPVPFIALLVSGGHTQLVLVNAIGDYELLG
                     ESVDDAAGEAFDKAAKMLDLGYPGGPNLARAAEGGDPHRFTFPRPMTDRPGLDFSFSG
                     LKTYTLNTTRDLAPMSDADRADVARAFEEAVVDTLYIKCRRAVEQTGITHLVMAGGVA
                     ANARLRRKLGEDLRAAVYYPPARLCTDNGAMIAYAGCLRLQAGERAPLAIETRARWPI
                     VELGRPGPPA"
BASE COUNT          469 a         1026 c         1103 g          522 t
ORIGIN      
        1 ggggaacctg gttcagctgc gaggaagttc gccgcggccg ggtatatgaa tgcgacccgt
       61 tgggattgcg gcaaccggtg gcgctgccgg cgctgggacg attccagcac gaggctgccg
      121 tggtcgatcc gctcaccggt accgtctaca tgagcgagga tgagcccgac gggcgcctct
      181 accgcttccg gcccacgttg cccaggcatc tcgagcggcc cgatctgagc gctggcgtcc
      241 tcgaggtcgc ttcactcgct ccggacggct ccggctcggt ggtgtggctg cgcgttccgg
      301 accccgaagc gggccgcaag cccacccgct ggcaggtgcc tgaaagcacc ggcttcgacg
      361 gcggcgaggg gatgagctac tgcgacggtg tggtctatct cgcgaccaag ggcgacaacc
      421 gggtctgggc tctcgatctc ggcgaggatc gcctggaggt gatctacgat caggccacgg
      481 ccgcaacgcc catacttggc ggcgtcgaca acgtggaaac caatgcatcc ggcgacgtgc
      541 tggtggccga agacggcgat gacatgcaga tcgtcgttct cccccgggag ggcgagccgg
      601 ttgccctgct gcaggtcact cgccagggcg gatcggaaat caccggcctg gcattcacgc
      661 ccgacatgtc acgcctttac ttcagctccc agcgcggctt ttccggtcgg gtgacgggcg
      721 gcatcactta cgaggtcacc ggcccctttg tgggctgact ttctcagaat gagccatgga
      781 cgtatatctg gtcggcggcg cggtccgcga tgagctgctc ggccgcccgg tgcgcgagcg
      841 cgactgggtg gtggtgggcg cctcccccga ggcgatgctg aaacgcggct ttcggcaggt
      901 gggccgtgat ttcccggtct ttctgcaccc ggacaccagc gaggagtacg cgctggcccg
      961 caccgagcgc aagacggggc cggggcacaa aggtttcgag tgtcacgccg ggcccgacgt
     1021 cactctggag caggacctgg agcgccgcga cctcaccgtg aacgccatcg cgcgggcgtc
     1081 ggacggctcc ctgatcgatc ccttcggcgg tcgatcggac ctggaagcga aagtgctcag
     1141 gcacgtctcg gtcgctttct ccgaggatcc gctccgggta tttcgggtgg cccggttcgc
     1201 cgcccagctc gatgatttca ccgtcgccga agagacccgg gctctcatgg ccgagctcgc
     1261 cggcagcggc gcgctggcgg agctctccgc ggagcgggtc tggcaggagc tggtcaaggc
     1321 actgggatcg aaggcgcccc tgcgcttttt caccgttctg gcatccgtgg gcggcttgtc
     1381 gacgtggttc gccgagctcg ccgaagtcga gatcaccttc ccggaagcaa tcaaagatgc
     1441 gctcggtcgg ttcgccgccc ttggctggca tctcgccccg gaggcggtag atacgctatg
     1501 tgaacggctt cgggcgccgc gccgacaccg ccgggccatg ggccacatcg cctctcacgg
     1561 ggtggtactt gctgcctggg agaccgccga ggcgggaagc ctgtgcgcgg cgctgaagtc
     1621 cgtgggggca ttcaaggcgg gtgacgatcc agagatggcg gtgcgcgttt cggccgcctg
     1681 cgcggggcgc aatcccgagc cgctgctcga gctggcggcc tcgattcgtc aggaggtgac
     1741 gacggccagc gttggaggct ccctgaccgg agccgctctg ggcgaggcca tcgaccaggc
     1801 gcgcacgcag gccattcgcc aggcccagcg ttcgagcgtc ggcaggtagg ccgggtcgag
     1861 acgtgcctct tgctgatgtg ttgattcgtt gggcgaaagg tcaggcccgc gaacgctcga
     1921 ttttcacgcc caccgtctcc gcgtctgtga ccgccccggg cttgagcact tccaccacca
     1981 cgcgcgcggc gggatagcct tccagcagca gcgccgcgat gccgtcggcg agcgcttcga
     2041 tgagctcgaa gcggctgccg cggacgaact ccgcgacccg gctcgccgct tcgccgtagt
     2101 cgagggcgtc ttccaggtca tcgctttggg cggctgcccg ggtatcggca aacagctcga
     2161 aggagacgat caccctttga ggccgggtgc gttcttcggg gtagacgccc acgatggtgg
     2221 agacggtcag gttcctgacg aagacgctgt ccatgggtca ggccggcggg ccggggcggc
     2281 cgagctcgac gatgggccag cgcgcacggg tctcgatggc aaggggcgct cgctcgcccg
     2341 cctgaaggcg caggcagccg gcgtaggcga tcatcgctcc gttgtcggtg cacagccgcg
     2401 ccggggggta gtagacggcg gcccgcaagt cttcgccgag ctttctgcgc agccgggcgt
     2461 tggccgccac gccgccggcc attaccagat gggtgatgcc cgtctgctcc accgcccggc
     2521 ggcacttgat gtagagggta tccaccacgg cctcctcgaa cgcgcgcgcg acgtcggcgc
     2581 ggtctgcgtc gctcatgggg gccaggtcgc gggtcgtgtt cagggtgtag gtcttcaatc
     2641 cgctgaagct gaagtcgagc ccggggcggt cggtcatggg tctcgggaac gtgaatcggt
     2701 gcggatcgcc cccttccgcg gcgcgcgcca ggttgggacc tccgggatag ccgagatcca
     2761 gcatcttcgc cgccttgtcg aaggcctccc cggcggcgtc gtccacggac tcaccgagga
     2821 gctcgtaatc gccgatcgcg ttgaccagca ccagctgggt gtgtcctccg gaaaccagca
     2881 gggctatgaa cggcacaggc ggagccgtgt ccggattcat cagcggggcc agcaggtgcg
     2941 cctccatgtg atgcaccgcg acggccggtt tgcccagcga ccaggccagg ctcctcgcaa
     3001 aacccgcgcc caccagcaac gcgcccatga gcccggggcc ggacgtgtag gcgacgccgt
     3061 caacgtccgc gagggagcgt ccggaatcgt ccagcgtctt gcggacaaga ggaaccagct
//
LOCUS       DBNHOX010000035        13150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_646057, whole genome shotgun sequence.
ACCESSION   DBNHOX010000035 DBNHOX010000000
VERSION     DBNHOX010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13150
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_646057"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="AC16UZ_00695"
     CDS             <1..1105
                     /locus_tag="AC16UZ_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6245222.1"
                     /translation="SAAAVGTLFFVSKLWDAVNDPLMGALADRTNTRHGKYRPYLLWM
                     ALPYGLMGYLMFANPDFDDTGKLVYAWVTYSLMMMLYTAINVPYSALMGVMTPSTTER
                     TLLASYRFVGAFGGGLLISMLVRPMVHYFGAEDEAAGFQVTMALFAVISVVMFWYSFG
                     ATRERVRSLPETDVSFGNDVKLLLSNRPWIVLFFAGIFTLSSIAIKNAVTIHYFKYYV
                     GDDSSPILLFFDITTLFMTIGMLALIAGVACTKWLTARFEKKTLLIALSLGNAVTMVA
                     FFFVPPDQLALMLALNITGSLMAGPTAPLVWAMYADVADYGEWRFERRTTGLVFSASM
                     FAQKFGLTIGAGLAGWMLALFGYVANVSQSDDR"
     gene            1105..1260
                     /locus_tag="AC16UZ_00700"
     CDS             1105..1260
                     /locus_tag="AC16UZ_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245223.1"
                     /translation="MGIRLMFTLFPAAVSLLNVAVIAFYTLSDAEVESIGRELEDRKA
                     QAGPAPI"
     gene            1325..3388
                     /locus_tag="AC16UZ_00705"
     CDS             1325..3388
                     /locus_tag="AC16UZ_00705"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6245224.1"
                     /translation="MAEVSEQMPQLFISYSRDDGRAVYPDADWLGGLGYAVHIDRHIS
                     PGEQWREAIANAIADSDLLIFYASPSSIESKFCSQELQFALSRDIPVITVMLEETPFP
                     PWMELALGNHQILQRFDVPQRAYRSLLESGIANQLDQIPPAAPPLTSPRGRWRGMWIG
                     AGAITALVFVLWRSFLPEPLAIAVAPFETDGSLRAAEMAGGVTAELSQGLTNLDLMPV
                     KSRLPDRRPAAGAFVGGSNDASHVIIGHVQLLDPGYLIRTSLIATEDDQEIWAQTFVY
                     VEDDPKEVQHQHVQNLLVALPDVLVGTIPALDRAAPSARAAAQQIYGEGARMLAQPGE
                     LQHVDTIRQLFEQALDNQPGFGLALLGLCDAHARTYQESRELDDLLHAETRCLQALAV
                     GSEPLKAHLALGRVFLARGEAQLARFHFQRASAVAPGLAEPLIGLADVADQNGDLEMA
                     RAYYEQALALPGTSWEAHLAYGNHLLYQGEFRAAAEQYEVVADLYPAAAIAYVNLGAA
                     WYSLGEFERASQAWRKSVELEPNPMSWGNLGAAQTYARNFSAAANAYREAAALAPSDH
                     RWLGHLGEILAYDPTGDAEEAQRILQQALDLALEELAFTREEQGPVQARISTYEALLG
                     RDADAMARARRVRAEHEDDMTVAYSVLQTYTLLNRATLAARERERLLGLGYPALLLER
                     DPWLN"
     gene            3438..4010
                     /locus_tag="AC16UZ_00710"
     CDS             3438..4010
                     /locus_tag="AC16UZ_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245225.1"
                     /translation="MNAQLSTTSIKRLSVKAVLACLACIGLAGCVSESTTNTWGTQLF
                     SVSPDNFSCSKAPDMRLKIEQTAGDASGILCVTGGGDVNCTNPAPNGPAVVLRNQLIQ
                     WELSSGSRTEPFYIIFDEDKSPAQKGKNRGANVVASENGKLCMRVNGRATASAQGEPY
                     RYSVYVKHCAGAPTCTPKVLDPIIYVRKVY"
     gene            complement(4047..4421)
                     /locus_tag="AC16UZ_00715"
     CDS             complement(4047..4421)
                     /locus_tag="AC16UZ_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245226.1"
                     /translation="MAEQQHQAGKLDVGALIHSSGGVLCLVLGGLMYLDGFAWAVHAF
                     SAMMLLTFVSALIISINTDSWVAGHYLAMSPVVGVVLGYADFDWGFTVAYLLIWAAFL
                     HFLWRGYQAVQAAKRESGTRRV"
     gene            4583..5431
                     /gene="yghU"
                     /locus_tag="AC16UZ_00720"
     CDS             4583..5431
                     /gene="yghU"
                     /locus_tag="AC16UZ_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020800808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HIR6245227.1"
                     /translation="MTKSSAYVPPKVWKWDTESGGRFANINRPTAGATHDKELPVGEH
                     PLQLYSLATPNGVKVTVMLEELLDLGKTDAEYDAWLINIGDGEQFGSGFVAINPNSKI
                     PALLDRSTETPTRVFESAAILLYLAEKFGAFLPEDLSSRGECFSWLFWQMGSAPYLGG
                     GFGHFYAYAPEKFEYPINRYTMEVKRQLDVLDQNLAQRRYLIGDEYTIADMATYPWYG
                     AVVLHNIYDAAEFLDVTSYEHVIRWAKEIEERPAVQRGRRVNKVWGSEETQMPERHSA
                     SDFGSV"
     gene            5460..6806
                     /gene="gdhA"
                     /locus_tag="AC16UZ_00725"
     CDS             5460..6806
                     /gene="gdhA"
                     /locus_tag="AC16UZ_00725"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014755289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HIR6245228.1"
                     /translation="MTITAPDLDQFMRGLRRRNPGESEFHQAVQEVAIDILPYIADKP
                     AYTAKRIFERMTEPDRIVSFRVVWEDDAGNVRVNRGYRVQCNNAIGPYKGGIRFDPSV
                     NQSILKFLAFEQTFKNSLTGLPLGGAKGGADFDVKGKSDSEVMRFCQAFMTELYRHIG
                     PFTDVPAGDIGVGSREVGYLFGQYKRLANRFEGVLTGKALEFGGSHIRTEATGYGVVY
                     MMENMLAHRGESLEGRTCAVSGSGNVAIYTVEKLNELGARVVTMSDSSGFIHDPDGID
                     ADKLAWILDLKTVRRGRVSEYADEFGVEFQEGKRPWAVPCELAFPCATQNEIGKEDAE
                     TLVANGCCAVVEGANMPTVPEALEVFTSARTLLAPSKAANAGGVGVSGLEMSQNSVRL
                     GWTREEVDTRLRQIIKEIHGHCVEHGDEGDYVNYLKGANIAGFRKVAAAMLAYGVM"
     gene            complement(6815..8869)
                     /locus_tag="AC16UZ_00730"
     CDS             complement(6815..8869)
                     /locus_tag="AC16UZ_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6245229.1"
                     /translation="MQRLFFGLCSCFAAASGQAQPSSSEPIEEVVVIAHPLSGEGLAQ
                     AATVLEGRELDRKRASTVGETLGREAGIHSAQFGKAVGRPVIHGLGGARVRVMEDRID
                     AMDVSVTSADHAVTVEPFVADRVEVLKGSSALLYGSGAIGGVVDVHTGRIPHEVPDRP
                     LTGGIETRFDSNNDGNATSGKLDGAVGDFAWHVDGTWKDGDDYEIPGFAESEGLRALK
                     SAATSGSLGEEDEAHDRLPGSAFDSESYAVGLSRVGEWGFLGGSVSRLEADYGLPGGH
                     GHAEDGDAGGGLGDDHGDTPTLALKQTRTDFELGILDPFGSFTSANLRLGINDYEHAE
                     IEPDGAVATEFANEAWELRAELVYELEAWRGALGLQHSDRDFSAIGEEAYVPPVQTTD
                     SGLFWVAERDFGGFDLEAGARIGRLEHEPLGAPSERFTTYGLSLGAVVPLGAGWRLGL
                     LGDLSSRAPVAEELYSDGPHLATRAFEIGDPALDSEKAFNLSATLEFEGERWSLAGTV
                     YYTGFSDFIYQQATGGVEDGLPVYVYRQEDARFVGVDASVEWRAAAWEGGDLRLRGLF
                     DLVDAKLDVSGNDNLPRIPPMRYGVGAQMSFGPVTASLDYLRVEDQDDAAPGELPTQG
                     YDDLQAYLGVELPFAADHRLTMFVSGRNLTDDEQRLHTSFIKDFAPAPGRTIEAGLRY
                     LF"
     gene            complement(8945..9217)
                     /locus_tag="AC16UZ_00735"
     CDS             complement(8945..9217)
                     /locus_tag="AC16UZ_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245230.1"
                     /translation="MTRLRSVLLVLTLLVFSSQSIAHDHLSDDLEMDPSCLVCSYSDS
                     VPLTGPVSLTPLWADPAGAKAWPPALGPTPRDCPKDYLSRGPPFSR"
     gene            9366..10247
                     /locus_tag="AC16UZ_00740"
     CDS             9366..10247
                     /locus_tag="AC16UZ_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6245231.1"
                     /translation="MQTWQVGDVKVTQIVELVFTGLDAFLPDATPEAVLPIEWLRPHF
                     ITPEGVLRFSIHALVIDTGARKIIVDTCVGNDKPRDVFPDWHMLQTGFLEDLASAGYP
                     RESIDTVLCTHLHLDHVGWNTMLEGGQWVPTFPNARYLLERSEFAYVAQEAEAEDVEP
                     WLRDMNRAVMADSIRPVVDAGLIDLVDADHRLCEEVRLMPTPGHTIGHVSVHIASRGE
                     SALITGDFIHHPCQLAHPEWSVTTDYDPSDSVSTRERVFAELADGPTLVIGTHWPEPT
                     AGKIASWGDTYQLKTDP"
     gene            complement(10303..11751)
                     /locus_tag="AC16UZ_00745"
     CDS             complement(10303..11751)
                     /locus_tag="AC16UZ_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245232.1"
                     /translation="MQSAQPDGQVLVADARAASEVLHEVLADTAAAFPGALGPMGFPA
                     DPKAFRSTYPEVMPRFEAARLASGDRLAMARHAVGALQRRLVWQDAGGSRPLADALAE
                     PAAPLELLQTDFPGEPGWRPSVVYRGERWEADRLADFGAELLGRGVVTAQASQALAWL
                     ADNVLDAGVLRLTGRKIAVLGGGAEMAPTRLWLEAGADVLWLDVAPPPPSWQDASAMS
                     GRLYWPAGNADLLAQPREILATLIEFAAGRPLDLGLYAYAPGRARELRLTGVMNELVN
                     AMPPELVGSITVLVSPTTPTGLAEPDLAAMRARREARPGWEAALDNLGLLGRGGGCAT
                     MGEVAATRTVVGIQGASYQAAQYLGKVLVAECWAEEGPLGSAHPAPFRVSANTAAITR
                     TRSLGHPVFAAAFGGAAAFGVETLTPRQSRRINGLLALCDWLRPDRPVPGTVRVHGGI
                     HTLPYPLESALRIAAAIGFARSPRLLRGLFGK"
     gene            complement(11761..12582)
                     /locus_tag="AC16UZ_00750"
     CDS             complement(11761..12582)
                     /locus_tag="AC16UZ_00750"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6245233.1"
                     /translation="MDLGLEGKKVIITGGSRGLGRAIGETFLREGAVVSFCSRTGRIE
                     PEDEDPRLGGEVRTDGVEQAAEKLAALGEVYGSVVDAADREQLARWVNEAAETMGGID
                     VVVSNTSALGGQPRSPEGWQRQFDVDIMSAVTLWDNAYPFLKASGAGAFVQVSTITAV
                     DYHAYGQACQSYGAFKGALVNYVCQLAYEYMSEGIRANCVSPGPIFVTGGSWDKIQRA
                     LPEYYEENVARQPAGRLGRPEEVADVVAFLASPRASWVTGENIVVDGAFTKYIKY"
     gene            12828..>13150
                     /locus_tag="AC16UZ_00755"
     CDS             12828..>13150
                     /locus_tag="AC16UZ_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245234.1"
                     /translation="MPRVLYSNANLLDGEHAARPGSSVIVEDGHILAVGAAESAAITA
                     HDELIDLEGRTLMPGLVMGHYHAAYRGYGEEGGSQTATAVEQAFFALGNVQIALKCGY
                     TSLISA"
BASE COUNT         2449 a         4397 c         4127 g         2177 t
ORIGIN      
        1 ctcggcggcg gcggtgggaa cgctgttttt cgtctcgaag ctctgggatg ccgtgaacga
       61 tccactcatg ggtgcgctgg cagaccggac caatacccgg cacggaaaat atcgcccgta
      121 cctgctctgg atggctctcc cctacggcct catgggctat ctgatgttcg cgaacccgga
      181 cttcgacgac accggcaagc tggtctacgc ctgggtgacc tattccctga tgatgatgtt
      241 gtacacggcc atcaacgtcc cctactccgc gctcatgggc gtgatgaccc ccagcaccac
      301 cgagcgcacg ctgctggcca gctatcggtt cgtgggtgcg ttcggtggcg ggctgctgat
      361 ttcgatgctc gtccgaccca tggtccacta cttcggcgca gaggacgagg ccgcggggtt
      421 tcaggtcacc atggcgctct tcgccgtgat ctcggtggtg atgttctggt actcgttcgg
      481 cgctacccgc gagcgcgtgc gttccctgcc cgaaaccgac gtcagcttcg gcaacgacgt
      541 gaagctcctg ctcagcaacc gaccctggat cgtgctcttc ttcgcaggga tcttcacgct
      601 gtccagcata gccatcaaga acgccgtcac gattcactac ttcaagtact acgtcggcga
      661 cgacagctcc cccatcctgc tcttcttcga catcaccacg ctcttcatga ccatcggcat
      721 gctggcgctc atcgctgggg tcgcctgcac caagtggctg acggcccgtt tcgagaagaa
      781 gaccctgctg atcgctctgt ctcttggcaa cgccgtgacc atggtcgcct tcttcttcgt
      841 cccgccggac cagctcgcgc tgatgctcgc gctcaacatc accggttcgc tcatggcggg
      901 acccacggcc ccgctggtct gggccatgta cgccgacgta gccgactacg gcgagtggcg
      961 ctttgagcga cgcaccaccg ggctcgtttt ctccgcttcc atgttcgcgc agaaattcgg
     1021 cctgaccatc ggcgccgggc tggcaggctg gatgctcgcg cttttcggat acgtagccaa
     1081 cgtctcccag agcgacgatc gctgatgggt atccggctca tgttcaccct gttccccgcg
     1141 gcggtgagcc tgctgaacgt ggccgtgatc gccttctaca ccttgagcga cgccgaggtg
     1201 gaatccatcg gccgggagct cgaggaccgt aaggcacagg cggggcctgc tccgatctga
     1261 gcctcggtgg cagccatcgg cgcctctggc ataatcctga gttggggttt ccggggcggg
     1321 atccgtggca gaagtatcag aacagatgcc gcagctgttc atctcatact ctagagatga
     1381 cggcagagcc gtatatccag acgcggattg gcttggcggt ctcggatatg ccgttcatat
     1441 cgacagacac atcagtcccg gagagcaatg gcgcgaggcg atcgcgaacg ccatcgcgga
     1501 cagtgatctg ctgatctttt acgcctcccc gtcctccatc gagtccaaat tctgctccca
     1561 ggaattacag ttcgctctga gccgcgacat tcccgtcata acggtcatgc tggaagaaac
     1621 cccgtttcct ccatggatgg aactggcgct gggtaatcac cagattctgc agcgcttcga
     1681 cgtgccgcag cgtgcgtacc gatccctgct ggaatcaggg atcgcaaatc agctcgatca
     1741 gattccaccc gcggccccgc ctttgacgtc cccgcgcggt cgctggaggg gaatgtggat
     1801 cggcgccgga gcaatcaccg cgctcgtctt cgtcctgtgg cgaagcttcc tgcccgagcc
     1861 tctggctatc gccgtagcgc ccttcgaaac cgacgggagc cttcgagcgg ctgagatggc
     1921 ggggggcgtc acggcggaac tctcacaagg actcaccaac ctggatctga tgccggtgaa
     1981 gagccggctc ccagatcggc gccccgcagc aggcgcgttc gtcggcggca gcaacgacgc
     2041 aagtcatgta atcatcggac acgtccagct cctggacccc ggttacctga tccgcacgtc
     2101 tctcatcgca accgaggatg accaggagat ctgggctcag acgttcgtgt acgtcgagga
     2161 cgacccgaaa gaggttcaac atcagcatgt gcagaatctg ctggtggcct tgccagacgt
     2221 gcttgtcggc accattccag ccctggatcg ggccgcgccg agcgcgagag cggcggcgca
     2281 gcagatctac ggcgagggtg ctcggatgct ggctcagccc ggcgagctgc agcacgtgga
     2341 tacgatccga caactgttcg aacaggccct ggacaaccag cctggctttg ggctcgcgct
     2401 gttgggcttg tgcgacgctc acgcccgaac ctaccaggag agccgcgagc tagacgacct
     2461 gcttcacgcg gagacccgct gcctgcaggc gctggccgtg ggttccgaac cgctgaaggc
     2521 gcatctggcc ctaggccggg tctttctggc ccgaggcgag gcacagctcg cccgattcca
     2581 ctttcagcgt gccagcgcgg tggctcccgg ccttgcggaa ccactgatcg gcttggcgga
     2641 cgtggccgac cagaacggcg atctggagat ggctcgcgcc tactacgaac aggccctggc
     2701 gctcccggga acgagctggg aagcccacct cgcctacggc aatcatctgt tgtaccaggg
     2761 cgagtttcgg gcggccgccg agcagtacga ggtggtggcc gatctgtacc ctgccgctgc
     2821 aatcgcctat gtcaatctgg gcgcggcctg gtacagcctg ggcgagttcg agcgggcgag
     2881 ccaggcgtgg cgcaagagcg tcgagctcga gcccaacccc atgagctggg gaaacctagg
     2941 cgccgcacaa acttatgcgc gaaatttttc tgctgcggcg aacgcgtacc gggaggccgc
     3001 cgccctggca ccctccgatc accgctggct cggccatctg ggggaaatcc tcgcctacga
     3061 cccaaccggt gatgccgagg aagcccagcg tatcctccag caggcgctag atctcgccct
     3121 ggaggaactg gctttcacca gggaggaaca gggcccagtg caagcccgaa tcagcacgta
     3181 cgaagccttg ctcgggcgtg acgcggatgc catggcccgg gcaagacggg taagggctga
     3241 acatgaagat gacatgacgg tcgcctacag cgtgctgcag acctacacgc tgctgaaccg
     3301 ggcaaccctg gccgctcgcg agcgcgagcg gctgctcggc ctcggttacc cggcgctgct
     3361 gctggaacgt gacccgtggc tcaactgacg caaccagggg cgcgcaccgc gccagataac
     3421 ctcaaatcgg aggacgtatg aacgcacaac tttcaaccac ttctatcaag cggctgtcgg
     3481 taaaggccgt ccttgcctgc ctggcatgca tcgggcttgc cggttgcgtc tcggaaagca
     3541 cgacgaacac ctggggtacg cagctcttct cggtcagccc ggacaatttc agctgttcaa
     3601 aggcccctga tatgaggctg aagatcgagc agaccgccgg ggacgcaagc ggaatactgt
     3661 gtgtcaccgg cggcggggac gtcaattgca cgaatccggc tccgaacggt cccgcggtgg
     3721 tgcttcgcaa tcagctcatt caatgggaat tgagcagcgg cagcaggacc gagcccttct
     3781 acatcatctt cgatgaggac aaatcacccg ctcagaaggg caagaaccga ggcgccaacg
     3841 tcgtcgcctc ggagaacggc aagctctgca tgcgcgtgaa cgggcgggcg acggcaagcg
     3901 cccagggcga accgtaccgc tacagcgtgt acgtcaagca ctgcgcaggc gcgccaacgt
     3961 gcacgcccaa ggtattggac cccatcatct acgtcaggaa ggtttattag ccgctaagga
     4021 ggcggccatg cctgaacggc cgcgctctac acgcggcgcg ttccggattc ccttttcgcc
     4081 gcctgaacgg cttgatatcc acgccagagg aagtgcagaa aggccgccca gatgagcagg
     4141 taggcgacgg tgaatcccca gtcgaagtct gcgtaaccta agaccacgcc gaccactggc
     4201 gacatggcca ggtagtgacc ggcgacccag ctgtcggtgt tgatggagat gatcagcgcg
     4261 ctgacgaacg tcagcagcat catggcggag aaggcgtgca cggcccaggc aaaaccgtcg
     4321 aggtacatca ggccgccgag cacgaggcac agcacgccgc cgctgctgtg gatgagggct
     4381 cccacatcca gcttgcccgc ctggtgttgc tgctcggcca tttggccctg ccacccgtgt
     4441 tctgcggcct gcgcaggcct tgaccgatca agttagcacg aaacgcggcc ggtggcaggc
     4501 caggtgtgaa agtctggtac attgagcacc cgtccggggg gccaccacgt gacgtgacac
     4561 acaatcaatc gggggtaaca ccgtgacaaa atcatccgcc tacgtcccgc cgaaagtctg
     4621 gaagtgggac accgagagcg gtggacgctt cgccaacatc aaccggccaa ctgctggcgc
     4681 gacgcacgac aaggagctgc cggtcgggga gcatccgctg cagctctact ccctggcgac
     4741 accgaacggc gtgaaggtca ccgtgatgct ggaggaactc ctcgatctcg gcaaaaccga
     4801 cgcggagtac gatgcctggc tgatcaacat cggcgacggg gaacagttcg gaagcgggtt
     4861 cgtggcgatc aaccccaatt ccaagatccc cgccctgctc gatcgcagca ccgagacgcc
     4921 cacacgggta ttcgagtctg ccgccatcct gctctacctg gcagaaaagt tcggcgcttt
     4981 cctgcccgag gacctctcct cccggggcga gtgcttctcc tggctgttct ggcagatggg
     5041 cagcgcgccc tacctgggcg gaggtttcgg ccacttctac gcctacgcgc cggagaaatt
     5101 cgagtacccg atcaaccgct acaccatgga ggtcaagcgc cagctggacg tgctcgatca
     5161 gaacctggcg cagcgccgct atctgatcgg cgacgagtac accatcgccg acatggccac
     5221 ctatccctgg tacggcgccg tggtgctgca caacatctac gacgccgccg agtttctcga
     5281 cgtcacgtcc tacgagcacg tgattcgctg ggccaaggag atcgaggagc gccccgccgt
     5341 tcagcgcggc cgccgggtca acaaggtctg ggggtcggag gagacccaga tgccagagcg
     5401 tcacagcgcc agcgatttcg ggtcggtttg atgggcttgt cgaggtcaga agaagcgcca
     5461 tgacaatcac cgctcccgat ctggaccagt tcatgagggg cctgcgccgc cgcaacccgg
     5521 gcgagtccga gtttcaccag gcagtgcagg aagtggccat agacatcctg ccgtatattg
     5581 ctgacaagcc cgcctacaca gcaaagcgga tcttcgagcg catgacggaa ccggatcgca
     5641 tcgtctcctt cagagtcgtc tgggaggacg acgccgggaa cgtgagggtg aaccgcggct
     5701 atcgggttca gtgcaacaac gccattggac cttacaaggg cggcatccgc ttcgacccgt
     5761 cggtcaacca gagcatcctc aagtttctcg ccttcgagca aaccttcaag aacagcctca
     5821 ccggtctgcc cctggggggt gccaagggcg gcgcggactt cgacgtaaag ggaaagtccg
     5881 actcggaagt catgcggttc tgtcaggcgt tcatgacgga gctgtaccga cacatcgggc
     5941 cttttaccga cgtgcctgcc ggagacatcg gggtcgggtc ccgggaggtc ggctacctgt
     6001 tcggtcagta caagcggctg gccaaccgct tcgagggcgt gctgaccggc aaggcgctcg
     6061 agttcggtgg gagccacatc cgcaccgaag ccaccggcta cggcgtggtc tacatgatgg
     6121 agaacatgct cgcccatcga ggggagagcc tcgagggcag aacgtgtgcc gtatccggct
     6181 ccggcaacgt ggccatctac accgtggaga agctaaacga gctcggcgcc agggtggtca
     6241 ccatgtccga ctcatcagga ttcattcacg acccggacgg gatcgatgcg gacaagctcg
     6301 cctggattct ggatctcaaa accgtccgcc gcggtcgcgt gagcgagtac gcggacgagt
     6361 tcggcgtcga gttccaagaa ggcaaacggc cctgggcggt gccctgcgag ctcgcatttc
     6421 cctgcgcgac ccagaacgag atcggcaaag aggatgccga gaccctcgtt gccaacggct
     6481 gctgcgcggt ggtcgagggc gccaacatgc ctaccgtgcc cgaagcgctg gaggtcttca
     6541 ccagcgcccg aactctgctc gcgccctcca aggcggcgaa cgcgggcgga gtcggcgtgt
     6601 ccgggctgga gatgagtcag aacagcgtgc gtctgggctg gacgcgcgag gaagtcgaca
     6661 cgcgcctgcg ccagatcatc aaagaaattc acggtcactg cgtggagcat ggcgacgaag
     6721 gcgactacgt gaactacctc aagggcgcga acattgccgg cttccggaag gtggcggcgg
     6781 ccatgctggc ttatggggtg atgtgagaag cccgtcagaa caggtaacgc agcccggctt
     6841 cgatggtacg gccgggcgcc ggggcgaagt ctttgatgaa cgaggtgtgc agacgctgct
     6901 cgtcatcggt caggtttctg ccggagacga acatcgtcag gcgatgatcg gccgcgaaag
     6961 gcaactccac acctaggtat gcctgaaggt cgtcgtaccc ctgggttggc agctcccccg
     7021 gcgccgcgtc gtcctgatcc tcgaccctga gataatccag cgaggccgtg acaggcccga
     7081 acgacatctg ggcgccgacg ccgtaacgca tcgggggtat gcggggcagg ttgtcgttgc
     7141 cgctgacgtc gagcttggca tcgacgaggt cgaacagccc ccgaaggcgg agatcgcctc
     7201 cttcccaggc agcggcccgc cactcaaccg aggcgtccac gcccacgaag cgcgcatcct
     7261 cctgccggta gacgtagacc ggcaggccgt cctccacgcc tcccgtcgcc tgctgataga
     7321 tgaagtccga aaaaccggtg tagtaaacgg ttccggccaa cgaccagcgc tcgccttcga
     7381 attccagggt cgccgacagg ttgaaagcct tctcggagtc cagtgccggg tccccgatct
     7441 cgaaagcgcg ggttgcaagg tgaggcccat cggaatagag ctcctcggca acgggcgccc
     7501 gggaagagag gtcacccaga agaccgaggc gccatcccgc cccgaggggc accacggcac
     7561 caagggagag cccgtaagta gtgaaccgct cgctcggcgc gcccagcggc tcatgctcca
     7621 gccggccgat gcgcgcgccc gcttccaggt cgaaacctcc aaagtcccgc tcagcaaccc
     7681 agaacaggcc gctgtcggtc gtctgcaccg gaggcacgta ggcctcttcc ccgatggcgg
     7741 agaaatcacg gtctgagtgc tgcagcccga gcgccccgcg ccaggcctcg agctcgtata
     7801 ccagctcggc ccgaagctcc caggcctcgt tggcgaactc cgttgccacc gcgccgtccg
     7861 gttcgatctc tgcgtgctcg tagtcgttga ttcccagccg caaattggcg ctggtaaacg
     7921 acccgaacgg atccaggatg ccaagctcga agtcggtacg cgtctgcttc agggccaggg
     7981 tgggcgtgtc cccgtgatcg tccccgagac ccccgcccgc atcgccatct tcggcgtggc
     8041 cgtgcccccc aggcaggccg taatcggctt cgagccggct cacggacccg ccgaggaagc
     8101 cccactcacc cacccgggaa agaccaaccg cataggattc cgaatcgaac gcgctgcccg
     8161 gcagcctgtc atgggcctcg tcctcttccc ccagcgaccc gctcgtcgcc gccgatttca
     8221 gcgcacgcag accttcggac tccgcaaagc ccgggatctc gtagtcgtcg ccgtccttcc
     8281 aggtaccatc gacgtgccag gcgaaatcac ccacggcgcc gtcgagcttg ccgctggtgg
     8341 cgttgccgtc gttgttgctg tcgaagcggg tttcgatccc gccggtcaac ggccggtccg
     8401 gcacctcgtg gggaatgcgg cccgtgtgaa cgtccaccac gccgccaatg gcgccagagc
     8461 cgtagagcag cgcgctggat cccttcagaa cctccacgcg gtcagcaacg aacggttcca
     8521 ccgtgaccgc gtgatcggcg ctggtcaccg acacgtccat ggcgtctatg cggtcctcca
     8581 tcacgcgaac ccgcgcgccg cccagcccgt gaatgaccgg acgacccacg gctttgccaa
     8641 actgagcgga gtgaatgcct gcctcccggc cgagcgtctc gccgacggtg gaagcgcgct
     8701 tgcggtcgag ctcgcgccct tccagcacgg ttgcagcctg agcgaggccc tcaccggaga
     8761 gagggtgagc gatcacgacg acctcttcga tcggctccga tgatgatggc tgtgcctgcc
     8821 ccgacgcggc ggcgaagcaa ctacacagcc cgaaaaacaa tctctgcatg tcctgttccc
     8881 atcgattggc gctcgcccga cgaaagcagg ctgcggcatc aactcatctg aatgcgtgct
     8941 ggaatcagcg ggaaaagggc gggccgcggc tcaggtagtc cttggggcaa tcgcggggcg
     9001 tcgggcccaa agccggaggc cacgccttgg cccccgccgg gtctgcccaa agcggtgtca
     9061 gggagaccgg acccgtcagg ggcaccgagt ctgagtagct gcagacgagg cacgagggat
     9121 ccatctcgag atcatcggac aaatgatcgt gcgcgatgct ctggctcgag aagacgagca
     9181 gcgtcagcac cagcagaacc gatcgtaggc gcgtcatcgg caagaaagcc gttcctgaca
     9241 tcaacgggag tgcggggcgg aaactatcag cgatgcaggc acggcgcaag ccgagcaccg
     9301 gccaaccagc catgttctaa tctctccgtc tgaagcgcga tggccacgga tcaaggaaac
     9361 gcccagtgca gacgtggcag gtcggagacg tcaaggtcac ccagatcgtt gagctggtgt
     9421 tcaccgggtt ggacgctttc ctgcccgatg cgacgccgga agccgtcctg cccatcgagt
     9481 ggctgcggcc gcatttcatt acccctgagg gcgttctgag attcagcatt cacgcgctgg
     9541 tcatcgatac cggagcaagg aagatcatcg tcgacacctg tgtcggcaac gacaaaccga
     9601 gggacgtgtt ccccgactgg cacatgctgc agaccggctt cctggaagat ctggcctcgg
     9661 caggctaccc gcgagagtcg atcgatacgg tgctctgcac gcatcttcac ctggatcacg
     9721 tgggttggaa caccatgctc gaagggggcc agtgggtgcc gaccttcccc aacgcccgct
     9781 acctgctcga acgcagcgag ttcgcctacg tggcccagga agcagaagcg gaagacgtcg
     9841 agccctggct tcgcgacatg aaccgagccg tgatggcgga ctccatccgc ccggtggtgg
     9901 acgccggcct gatagatctg gtggatgccg accatcgcct gtgcgaagag gtgcggttga
     9961 tgccgacgcc cggccatacc atcggccacg tcagcgtgca catcgcctcc cgcggcgagt
    10021 ccgccctgat caccggcgat ttcatccacc acccctgtca gctcgcccat ccggaatggt
    10081 ccgtgacgac cgactacgac ccttcagatt ccgtgagcac gagagagcgg gtctttgccg
    10141 agctggccga cggccctacc ctggtgatcg gcacccactg gccggaaccc accgccggga
    10201 aaatcgccag ctggggcgac acctaccagc tcaagacgga tccctgagct gccgctcccc
    10261 gaccccaacg gcccgagacc ggaacatcga gccccgagcc gatcacttcc cgaacaggcc
    10321 tcgcagcagc cgaggcgagc gcgcaaagcc gatggcggcc gcgatgcgca gcgccgactc
    10381 caacggatac ggcagcgtgt ggatgccgcc gtgcacccga acggtgccgg gcacgggccg
    10441 gtccgggcgc agccagtcgc aaagggccag aagcccgttg atgcgccgcg actggcgggg
    10501 agtcagcgtc tccacgccga atgccgccgc cccgccaaac gccgcggcaa agaccgggtg
    10561 acccagggag cgcgtgcgcg tgatcgccgc ggtgttggcg gacacccgaa acggtgcggg
    10621 gtgagcgctg cccagcgggc cttcctcggc ccagcattcc gccacgagca ccttgcccag
    10681 gtactgggcc gcctgatagc tggctccctg aatgcccacc accgtgcggg ttgccgcaac
    10741 ttctcccatg gtggcgcagc ctccccctct tcccagcagg ccgagattgt cgagcgcggc
    10801 ttcccagccg gggcgggctt ccctgcgcgc gcgcatggcg gctaggtctg gctcggcgag
    10861 gccggtcggc gtggtcggcg agaccagaac ggtgatgctg cccaccagct ccggcggcat
    10921 cgcgttcacc agctcgttca tgacccccgt gagtcgcagt tcccgcgccc gccccggggc
    10981 ataggcgtac aggccgaggt cgaggggcct gccggcggca aactcgatca gcgtagcgag
    11041 gatttcacga ggttgagcga gcagatcggc gtttccggcc ggccagtaca gtcgaccgga
    11101 catggccgac gcgtcctgcc agctcggcgg cgggggcgcc acgtcgagcc agagcacgtc
    11161 ggcccctgcc tcgagccaaa gccgcgtggg cgccatctca gcgccgccgc cgagaaccgc
    11221 gatcttgcgt cctgtcagcc tcagcactcc ggcatccagc acgttgtcag ccagccaggc
    11281 gagggcttgg gacgcctgcg cggtcacgac gccgcggcca agcagctccg cgccgaaatc
    11341 ggcgagccga tcggcttccc aacgctctcc ccgatacacc acgctcggcc gccagccggg
    11401 ctcccccgga aagtcggtct gcaggagctc gagcggcgca gccggctccg ccagcgcgtc
    11461 ggccaggggc cggctaccgc cggcatcctg ccagaccagc cgccgttgca gggcgccgac
    11521 cgcgtggcgt gccatggcaa ggcgatcgcc gctggccaga cgggccgcct cgaaacgggg
    11581 catcacctcg ggatacgtgg aacggaatgc cttgggatcc gcgggaaacc ccatcggacc
    11641 cagggcgccg ggaaaggccg cggcggtgtc ggcaagcacc tcgtggagca cctcggacgc
    11701 agcgcgcgcg tctgcgacca ggacctgccc atcgggttgt gcggactgca ctgcgaacgg
    11761 ctaatacttg atgtacttgg tgaaggcacc gtccacgacg atgttctccc ccgtgaccca
    11821 ggacgccctg gggctggcca ggaaggccac cacgtcggcc acctcctccg gcctgcccag
    11881 ccgacccgcg ggctgacgcg ccacgttctc ctcgtaatac tcgggcaggg cccgctgaat
    11941 cttgtcccag gaaccgccgg tgacgaagat cggccccggg gatacgcagt tggcgcggat
    12001 gccttcgctc atgtactcgt aggcaagctg gcacacgtaa ttgaccagcg ctcccttgaa
    12061 agcgccgtag ctctgacacg cctgcccgta agcgtggtag tccacggcgg taatcgtcga
    12121 gacctgcacg aacgcgcccg ctcctgaagc cttgaggaac gggtaggcgt tgtcccacag
    12181 cgttaccgcg gacatgatgt ccacgtcgaa ctgccgctgc catccttccg gcgaacgcgg
    12241 ttgccccccg agcgcgctgg tgttcgaaac caccacgtcg atgccgccca tggtctcggc
    12301 cgcttcgttc acccagcgcg cgagctgctc gcggtcggcg gcgtccacga cggacccgta
    12361 cacctcgcca agggcagcca atttctctgc cgcctgctcg acgccgtccg tccggacctc
    12421 cccgccaagg cgcggatctt catcctcggg ttcgatacgc ccggtgcggg aacagaacga
    12481 cacgacggct ccttcgcgca ggaacgtctc cccaatggcg cgacccagcc cccgcgagcc
    12541 gccggtgatg atgactttct ttccttcgag cccaagatcc atgactcaca cccccaaaac
    12601 ccgatcaggc aagtatagcg aacccggcca gcgccgctgc cgtgcacccg actctcaccg
    12661 gttggagtgt tccccaaagt catccggcga acacgccgaa ctcacaggcg atctccggat
    12721 gcgtctggtc cgggccagtg atactgttgg gtgttggggg caagtcgtgc cgaggctcat
    12781 cacccatcgg ccgttgcacc gaccatctcc gccacgggag ggacaacatg ccacgagttc
    12841 tgtacagcaa tgcgaacctg ctcgacggtg agcacgccgc cagacccgga tcctcggtga
    12901 tcgtggaaga cgggcacatc cttgccgtcg gcgccgcgga gtccgcggcg atcaccgcac
    12961 acgacgagct gatcgacctc gagggtcgca cgctcatgcc cgggctggtc atgggccact
    13021 atcacgccgc gtatcgcggc tacggcgaag agggtggcag ccagacggcc accgccgtgg
    13081 agcaggcatt tttcgcgctc ggcaacgtgc agattgcgct caagtgcggc tacaccagcc
    13141 tgatcagcgc
//
LOCUS       DBNHOX010000036         3127 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_646068, whole genome shotgun sequence.
ACCESSION   DBNHOX010000036 DBNHOX010000000
VERSION     DBNHOX010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_646068"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="AC16UZ_00760"
     CDS             <1..653
                     /locus_tag="AC16UZ_00760"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF049302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6245235.1"
                     /translation="SRGFTKRANSVVPLYPWTGNGREPLAERVRHCENLYAREGLQTI
                     FRLPAIDDNTALDAHLESRGYNLVDPTLVLVAPTRPGPGHEDFALVPAEHWLDIYAEL
                     TGMPDTARKLHGAIVAGIPTPCGFAVLRRGERPVACGLAVVERDLLGLFDIVTDPDAR
                     RSGHGTALVGSLLGWGHARGARLAYLQMIESNAPASALYRKLGFEIAYNYWYRISP"
     gene            937..>3127
                     /locus_tag="AC16UZ_00765"
     CDS             937..>3127
                     /locus_tag="AC16UZ_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HIR6245236.1"
                     /translation="MSIRTPWGFPPKRGLYDPANEKDSCGVGFICDIKGRASRDIIDQ
                     AQHMNCCMTHRGGVGYERNTGDGAGILTGLPDKFLRRVAASELGVTLPPQGQYGVGNV
                     FLPNDEAERRHCIAIFEREIQAAGQKLLGWRELPVHPDGAGIGHAARAAMPTFAQLFV
                     GAKDASGDAFERKLYLIRKHSTHLLRGDMALGERKLFYVCSLSSKVIIYKGMLTPEQV
                     FPFYQDLDDPDYESHLAMVHSRFSTNTFPSWDRAQPNRFMSHNGEINTLLGNVNSMNA
                     RQGLLSSPLFGADIEKLYPIVEEDCSDSGTFDNVLESLLMSGRKLQEAVLMMIPEAWQ
                     QHPTMDQAKRAFYEYHSCLMEPWDGPASIAFTDGTYIGAVLDRNGLRPSRYYITNDDR
                     CIMASEVGVVPVDPACVLEKGRLQPGRIFLIDFEQGRMIPDAEVKQEWVKARPWQQWL
                     DNQRIDLAEIPPGDDPHGFHPANLTPRMQAFGYTVETMEIMLQPLVKELRDPLGSMGN
                     DAALAVMSDKPRMIYDYFKQRFAQVTNPAIDSIREEVIMALECYIGPERNLLETTEEH
                     AHRLRVPHPILSNEELAALKHMDHRGWRSRTIDITFKQGTGRDGLVAALDRICAEASG
                     AIADGYSLVVLSDRGIGPDRVPVSSLLAVGAVHHHLVREQSRTRIGIVLETGEAREVH
                     HHCLLVGFGADAINPYLAFEALWQARRNGYLDDAAHITSDDDVVAAYR"
BASE COUNT          584 a         1083 c          941 g          519 t
ORIGIN      
        1 tctcccgcgg cttcaccaaa agggcgaaca gcgtcgttcc cctctacccc tggaccggga
       61 acgggcgcga gccactggca gaaagggtgc gccactgcga aaacctgtac gcccgggaag
      121 gcctgcagac catattccgg ctgccggcca tagacgacaa caccgctttg gatgcgcatc
      181 tcgaaagccg cggctacaac ctcgtcgacc cgaccctggt gctcgtcgcg ccaacgcggc
      241 cgggcccggg ccacgaggat ttcgcgctcg ttcccgcgga gcattggctc gacatctacg
      301 ccgagctcac gggcatgccc gacaccgcgc gaaagctgca cggcgcgatc gtcgccggca
      361 ttccaacccc ctgcgggttc gccgtcctgc gacgcggcga gcgcccggtc gcctgtggcc
      421 tggcggtcgt cgagcgtgac ctgctcggcc tgttcgacat cgtcacggac cctgacgccc
      481 gccgttccgg tcacgggacc gccctggtgg gttccctcct cggctggggc cacgcccgcg
      541 gtgcccggct ggcatatctt cagatgatcg aatccaacgc gccggccagc gcgctctacc
      601 gaaagcttgg cttcgagatc gcatataact attggtaccg gatttcgccc tagccataac
      661 cttttgttcc taggcggccg aacttgattc atacccgcat ttttgagatg ttttgtcttt
      721 cgcgcggaca caccgcagct agcttgccag caaatatata agaatcagta agttataaac
      781 ggcaagcaga tctccgacag gacctgcgcg cttccgtggc ggaagccgac ggctgcggtc
      841 gcgcccgaca tccgctccca ctttgggcgg cgtcggcgtc tggctcccac cattgaggtc
      901 gaagcggccg ggccaaacac gaaagagaac agttgcatga gcattagaac gccatggggg
      961 tttcccccga agcgaggtct gtacgacccg gctaacgaga aagacagctg cggcgtcggc
     1021 ttcatctgcg acatcaaggg tcgcgcgagc cgggacatca tcgatcaggc ccagcacatg
     1081 aactgctgca tgacccatcg cggcggcgtc ggctacgagc gcaacacggg cgacggggcc
     1141 gggatcctga cgggcctgcc agacaagttt ctgcggcgcg tcgccgccag cgagctgggc
     1201 gtcacccttc ctccccaggg ccagtacggc gtggggaacg ttttccttcc caacgacgaa
     1261 gccgagcgcc gacactgcat cgccatcttc gagcgggaaa ttcaggcggc gggtcagaag
     1321 ctgctcggct ggcgggaact gcccgtgcac cccgacggcg cgggaatcgg ccacgcggcc
     1381 cgggccgcca tgcccacatt cgcccagctg ttcgtgggcg ccaaggacgc atccggcgat
     1441 gcgttcgagc gcaagctcta cctgatccgc aagcacagca cccatcttct gcgtggggac
     1501 atggcgctcg gtgagcgaaa actcttctac gtctgcagcc tctcctccaa ggtgatcatc
     1561 tacaagggca tgctgacgcc cgaacaggtc tttcccttct atcaggacct ggacgatccc
     1621 gattacgaat cgcatctggc catggtccac tcgcgtttca gcaccaatac cttcccgtcg
     1681 tgggaccggg cacagcccaa ccggttcatg agccacaacg gcgagatcaa taccctgctc
     1741 ggcaacgtca actccatgaa cgcccgccag ggtctgctca gcagcccgct gttcggcgcg
     1801 gacatcgaga agctctaccc gatcgtcgag gaggactgct cggattcggg caccttcgac
     1861 aacgtcctcg agtccctgct gatgagcggc cgcaagctgc aggaagcggt gctcatgatg
     1921 attccggagg cctggcagca gcacccgacg atggatcagg cgaaacgggc cttttacgag
     1981 tatcactcct gcctgatgga gccgtgggac ggccccgcat ccatcgcctt caccgacggg
     2041 acatacatcg gcgccgtgct cgaccgcaac ggcctgcgcc cgagccgcta ctacatcacc
     2101 aacgacgaca ggtgcatcat ggcctccgag gtcggcgtcg tgcccgtcga ccccgcctgc
     2161 gtgctcgaga aggggcgcct gcagcccggt cggatcttcc tcatcgactt cgagcagggt
     2221 cgcatgattc cggacgccga ggtcaaacag gagtgggtga aggcccgccc ctggcagcag
     2281 tggctggaca accagcgaat cgatctggcc gagataccac ccggcgacga tccgcacggc
     2341 ttccatcccg cgaacctgac gccgcgcatg caggcgttcg gctacaccgt cgagaccatg
     2401 gagatcatgc tccagccgct ggtcaaggag ctgcgagatc ccctggggtc catgggcaac
     2461 gacgccgcgc ttgcggtcat gtccgacaag ccgcgaatga tctacgacta cttcaagcag
     2521 cgcttcgccc aggtgaccaa cccggccatc gactccatcc gcgaggaggt gatcatggcg
     2581 ctggagtgct acatcgggcc ggaacgcaat ctgctcgaaa ccaccgagga gcacgcccac
     2641 cggctccggg tgccgcaccc gatactttcc aacgaggagc tggccgcgct caagcacatg
     2701 gaccaccgcg gctggcgttc gcgcaccatc gacatcacct tcaagcaagg caccggcaga
     2761 gacgggctcg tggccgcgct cgaccggatc tgcgcggaag ccagtggcgc catcgccgac
     2821 ggctacagcc tcgtcgttct ctcggaccgc ggtatcgggc cggatcgggt cccggtgagc
     2881 tcgctgctcg ccgtcggcgc cgtgcaccat cacctggtcc gtgagcagtc caggacccgt
     2941 atcggcatcg tgctggaaac cggagaagcc cgggaggtgc accaccactg cctgctggtg
     3001 ggtttcggcg ccgacgccat caacccgtat ctggccttcg aggcgctctg gcaagcccgc
     3061 cgcaacggct atctcgacga cgccgcccac atcacctcag acgacgatgt ggtcgccgcc
     3121 taccgca
//
LOCUS       DBNHOX010000037         3195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_388780, whole genome shotgun sequence.
ACCESSION   DBNHOX010000037 DBNHOX010000000
VERSION     DBNHOX010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_388780"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="AC16UZ_00770"
     CDS             <1..190
                     /locus_tag="AC16UZ_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIR6245237.1"
                     /translation="TAAALTAHVLGADPERSAQLAVAFVVFRVLHGVCYIGGWATLRS
                     LMFMGSLVSLIWLFVLGA"
     gene            218..922
                     /locus_tag="AC16UZ_00775"
     CDS             218..922
                     /locus_tag="AC16UZ_00775"
                     /inference="COORDINATES: protein motif:HMM:NF015605.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6245238.1"
                     /translation="MQGLPEVSIGALLVAFLPSLALVAVMRAWSLQAGQALIANIRML
                     VQLLAIGYVLVFVFETENPWIVAAVLVMMMGVAAWIALRPLQSRSARKYLQSLTAISI
                     CGLAMLALVSQLVIDLPRWFEPRFVVPLAGMIFANAMNTVSLAAERFESEQAREVEYA
                     EARTTALDAALIPQVNALLAVGLVSLPGMMTGQILSGVDPLIAVRYQIVVMCMIFGSS
                     GLAAVAYLFAQRPRGA"
     gene            complement(933..1409)
                     /locus_tag="AC16UZ_00780"
     CDS             complement(933..1409)
                     /locus_tag="AC16UZ_00780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6245239.1"
                     /translation="MPSRRELIALTEGEIRDYLAEQKTLIIVSNGKDGYAHPVPMWFW
                     TDPDGCLYCTTFSKAQKVLNFRRDPKASLLVESGDEYTELKSVLIYAKAEVIEEFDAV
                     REALVNISTKGRGDVSEAERAQIRDAVGKTAEKRVLLKFTPERYVSWDHAKLGGRY"
     gene            1513..2385
                     /gene="tesB"
                     /locus_tag="AC16UZ_00785"
     CDS             1513..2385
                     /gene="tesB"
                     /locus_tag="AC16UZ_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HIR6245240.1"
                     /translation="MSQDQTVTDLIDLLDLEEIEKNHYRATSPSEGWQRVYGGQVLGQ
                     ALVAASRTVPEDRHAHSLHGYFLRGGDTTVPILYTVDRIRDGRSFTTRRVVAIQHGKA
                     IFNMAISFQIDEPGLSHQMPMPEVPDPEGLPDESELRKAWADKLPEPLEAAFDRERPI
                     EVRPIDPVDVFAPEKRPPQQMCWMKCRDRLPDDPGLHQCVLAYLSDWSLLDTATLPHG
                     VSFMQDNMQMASLDHAMWFHRPFRADEWLLYVQDSPSASGARGLNRGLIYNRAGELVA
                     SAAQEGLIRLHETD"
     gene            2510..>3195
                     /locus_tag="AC16UZ_00790"
     CDS             2510..>3195
                     /locus_tag="AC16UZ_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6245241.1"
                     /translation="MAEAAVNIEELTESVGSLRDGLESHRYICSDQIATAVYLAYHLH
                     KPILIEGPPGVGKTELAKTTAEMFALPLIRLQCYEGLDEAKAIYEWKYGKQLLYTQVL
                     KETLDEVLQGAKGFAESVTRLHEFGDIFFSEEFLEPRPLLKALKEEAGCILLIDEVDK
                     SDHEFESLLLEMLSDFQVSIPEIGTVKAVRQPPMVFLTSNNTREISDALKRRCLNLYI
                     PFPDVELEQR"
BASE COUNT          537 a         1067 c         1016 g          575 t
ORIGIN      
        1 cacggctgcg gcgctcactg cccacgtcct gggggcggac ccggagagat cggcccagct
       61 ggccgtggcc ttcgtggtgt tccgggtgct gcatggggtc tgttacatag gcggctgggc
      121 gaccctgcgc tccctgatgt tcatgggctc cctggtcagc ctgatctggc tcttcgttct
      181 gggcgcctga cccgtagcgc ccgggaacat agcgacagtg cagggcctcc cggaggtctc
      241 catcggcgcc ctgctggtcg ccttcctgcc gagtctggct ctcgtggcgg tgatgcgcgc
      301 ctggtccctc caggccgggc aggcgctgat tgccaacatc cgcatgctcg tgcagctcct
      361 ggcgatcggc tacgtgctcg tgttcgtgtt cgagaccgag aacccgtgga tcgtggccgc
      421 cgtgctcgtg atgatgatgg gcgtcgctgc ctggatcgcg ctgcgacccc tacagagccg
      481 cagcgcgcgc aagtatctgc agtccctgac cgccatatcc atctgcggcc tggccatgct
      541 ggccctggtg agccagctgg tcatcgacct gccccgctgg ttcgagccac gcttcgtggt
      601 gccgctggcc ggcatgattt tcgccaacgc catgaacacg gtgagcctcg cagccgagcg
      661 gttcgagtcc gagcaggccc gggaagtcga gtacgccgag gcacgcacga cggccctgga
      721 tgccgcgctc atcccccagg tcaacgccct gctggcggtg ggtctcgtgt ctctaccggg
      781 gatgatgaca ggccagatcc tgtccggcgt ggatccgctg attgccgtgc gctaccagat
      841 cgtggtgatg tgcatgattt tcgggtcttc aggattggcg gcggtcgcct acctcttcgc
      901 ccagcggccg cggggggcct gaagcctgcg cctcagtaac gcccgccgag cttggcgtga
      961 tcccagctga cgtatcgctc cggcgtgaac ttcagcagaa cgcgtttctc cgccgtcttc
     1021 cctaccgcat cacgaatctg ggcgcgctcc gcctcggaca cgtcgccacg gcccttggtg
     1081 ctgatgttga ccagcgcttc acggaccgcg tcgaactcct cgatcacctc cgctttggca
     1141 tagatcagca cgctcttgag ctccgtatac tcgtccccgc tctccacgag cagggacgcc
     1201 ttcgggtcgc gccggaagtt gagcaccttc tgggccttgc tgaacgtggt gcagtagagg
     1261 cagccatccg ggtcggtcca gaaccacatg ggcaccgggt gggcgtagcc gtccttgccg
     1321 ttggacacga tgatcagcgt cttctgctcg gccaggtagt ccctgatctc tccctcggtc
     1381 agggcgatca gctcgcggcg tgacggcatc cgggcaactc ctcggggcgg cggttaaagc
     1441 ggcgatcgcc attctccccg gcgggtcggc tgactacaat agcgctccct ccaagcccgc
     1501 cagaagccgc caatgagcca ggaccagacc gtcaccgatc tcatcgatct cctcgatctg
     1561 gaggagatcg agaagaacca ctaccgcgcg accagcccca gcgagggttg gcagcgggtc
     1621 tatggggggc aggttctggg gcaggccctg gtggccgcct cacgcaccgt acccgaggac
     1681 cgtcacgccc actccctgca cggctatttc ctgcgcggcg gcgacaccac ggtgcccatc
     1741 ctgtacaccg tcgaccggat ccgggacggg cgcagcttca ccacccgtcg tgtggtagcc
     1801 attcagcacg gtaaggccat cttcaacatg gccatctcct ttcagatcga cgagcccggg
     1861 ctgtctcatc agatgcccat gcccgaagtg ccagatccag aggggctgcc cgacgagagc
     1921 gagctgcgta aggcatgggc cgacaagctc cccgagccgc tggaggcggc cttcgaccgg
     1981 gaaaggccca tcgaggtgcg tcccatcgac ccggtggacg tcttcgcgcc ggagaagcgg
     2041 ccgccacagc agatgtgctg gatgaagtgc cgcgaccgcc tgcccgatga cccgggcctg
     2101 caccagtgcg tgctggccta cctttccgac tggtccctgc tggacaccgc caccctcccc
     2161 cacggggtga gcttcatgca ggacaacatg cagatggcga gcctggacca cgccatgtgg
     2221 tttcaccgtc ctttccgtgc cgacgagtgg ctgctctacg ttcaggacag ccccagcgcc
     2281 agcggcgccc ggggcctcaa ccgtggcctg atctacaacc gcgcaggaga gctggtggcg
     2341 tcagccgccc aggaaggcct gatccggctt cacgagaccg actgatcggc gatggccaac
     2401 ggatcgctcg ccagcgcggt gtacaaggct gaaggggagc agcgataata gcgggttttg
     2461 tgcagcgcga aatacgcacg cccaggtaag agccgggctg ggggaagcag tggccgaagc
     2521 ggcagtgaac atcgaagagc tcaccgagtc cgtgggcagc ctcagggacg gcctcgagtc
     2581 acatcgctac atctgcagcg accagatcgc cacggccgtc tacctcgcct accacctgca
     2641 caaaccgatc ctcatcgaag gccctccggg ggtaggcaag acggagctgg ccaagacgac
     2701 ggcagagatg tttgcgctgc cgctgatccg tctgcagtgc tacgaggggc tcgacgaggc
     2761 caaggccatc tacgagtgga agtacggcaa gcagctcctg tacacccagg tgctcaagga
     2821 gacgctcgac gaggttctgc agggagcgaa gggctttgcg gaatccgtga cgcggctgca
     2881 cgagttcggc gacatcttct tctccgagga gtttttagag ccgcgcccgc tgctcaaggc
     2941 cctgaaggag gaagccggct gcatcctgct catcgacgag gtggacaagt ccgatcacga
     3001 gttcgaatcg ctcctgctgg agatgctgtc cgatttccag gtgtccattc ccgagatcgg
     3061 cacggtgaag gccgtgcggc agccgcccat ggtgtttctc accagcaaca acacccggga
     3121 gatctccgac gcgctcaagc gccgctgcct gaatctgtac attccgttcc ccgacgtgga
     3181 gctcgagcag cgcat
//
LOCUS       DBNHOX010000038         6374 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1353095, whole genome shotgun sequence.
ACCESSION   DBNHOX010000038 DBNHOX010000000
VERSION     DBNHOX010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1353095"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="AC16UZ_00795"
     CDS             <1..642
                     /locus_tag="AC16UZ_00795"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR6245242.1"
                     /translation="DFFAGLGYQVLTHGQKAWNGVAVLSRLPVEIVQKGLPGQESAGS
                     RLITVRVDDRLEFTTVYCPNGKTLDHEDYAKKLAWFTSLSEYWRDRPSAGAVLCGDFN
                     VVPEAVDSWRGEAGDGRIFHTLEERAAFRSLLDAGLEDLFRMAKPDEQAFSWWDYRGG
                     SFHRGHGLRIDLILGSRDVAERVVDVRTDRDYRKKKDGLTASDHAPVIAEIDL"
     gene            802..1353
                     /locus_tag="AC16UZ_00800"
     CDS             802..1353
                     /locus_tag="AC16UZ_00800"
                     /inference="COORDINATES: protein motif:HMM:NF039725.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6316 family protein"
                     /protein_id="HIR6245243.1"
                     /translation="MLPEWFRSDEIFSDEGRWYFGARGGLHVGPYGDRELASARSLSV
                     SRRLRALRTDADRLRYVRKVLHEEWESLGVSASAAEAETTIDLSPPSEPVRSGERQRK
                     WCRSSRFFQIHGVWFVSTREGINIGPYGTQFEARKHERHLIALLVRARDPAEARRVIY
                     RFKHRPNLTGTPWSQTDEVARTG"
     gene            complement(1377..2555)
                     /locus_tag="AC16UZ_00805"
     CDS             complement(1377..2555)
                     /locus_tag="AC16UZ_00805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015270460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6245244.1"
                     /translation="MNQPDTALSPKVSELVARTTEFVTDKVIPYEKDPRNGSHGPSDA
                     LRVELQTAAREAGLLVPQLAEHLGGHDLNHREMAAVFRAAGYSPLGPIALNIMAPDEG
                     NMHLLHKVATPEQQERFLQPLAAGTIRSAFLMTEPDGGAGSDPGMLATMAEREGDDWV
                     INGRKSFITGGEGAGFGIIMAKTGSSATMFLADMNTPGISIERVLDTIDSSMPGGHAV
                     VRLDGVRVPADQILGELDEGFRYAQVRLAPARLTHCMRWWGSAQRAHDIASDYACRRQ
                     AFGKPIIDHEGVGFMLADNLMDLKQAELMIDWCAGVLDSGAPGTAESSMAKVAVSEAL
                     FRVADRCVQVLGGTGVTQDTIVEQVFREIRAFRIYDGPSEVHRWSLAKRIKRERLAST
                     "
     gene            complement(2552..3436)
                     /locus_tag="AC16UZ_00810"
     CDS             complement(2552..3436)
                     /locus_tag="AC16UZ_00810"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6245245.1"
                     /translation="MGRPTDLGVIDLMLEMPTGTAGMGMKQARALTRDKGTGEFSHHP
                     AQYLFKDAPERMSLATDPDAVVGMMDDFGVAMAQIAVNPHKPEAALDLFEKHPGRFFG
                     EVGVDPNSGMEGVRALETCVGLHPNIKAASAAPCLLNPQVPIDDKKFYPIYAKCCELD
                     IPINMLVGVPGPRVPYRCQHPGLLDEVAWFFPELKVVMRHGGDPWTDLCVKLLLKWPN
                     LYYSTSAWAPKHYPRNVLEFANKRGRGKVLYAGYFPGLSYERIFTEMDDLPLTDDTWP
                     AFLRDNAINVYKLQDILE"
     gene            3544..4173
                     /locus_tag="AC16UZ_00815"
     CDS             3544..4173
                     /locus_tag="AC16UZ_00815"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6245246.1"
                     /translation="MIRIYHVAGTRSVRVIWLCEELGLPYEKVAVDFSPAYRATPEWR
                     RLNPVGKVPVLVDDDVTMFESGAMVEHLLDRHGAGRLRPPPGTAERAAYLQWSWFAEA
                     TFARPLGEIVNHRREFPGESEIPAVLEEMSARAALCVQALDEALAGRQYLLGDEFTAA
                     DVMMGYSVLLARALGIEGARAGRVGAYFDVLARRSGYPAATADLPDAWR"
     gene            4219..5082
                     /locus_tag="AC16UZ_00820"
     CDS             4219..5082
                     /locus_tag="AC16UZ_00820"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6245247.1"
                     /translation="MSRSTFDVRPLDASFGAVVTGLRLADLGDDAFFDLYDAWLQHAL
                     LIFPDQHLSNAEQVRFAQRFGALEFDLTPLSNVRGDGSLRPDDGSDDVVKVLRGNMGW
                     HCDSTYMPVQAKGAVFTAHVVPDEGGETGWADMRAAYDALDEEMRERIADLCAYHSLY
                     HSQAKVGHVPSSESAYSNYGFHEGEPPLRPLVKIHPETGRPALMVGRHAYGIPGMDAD
                     ASERLLDELVEFACRPPRIYHHTWSPGDAVVWDNRCLMHRACPWDMSKPRVMYHARIA
                     GDPASESGLAA"
     gene            complement(5139..>6374)
                     /locus_tag="AC16UZ_00825"
     CDS             complement(5139..>6374)
                     /locus_tag="AC16UZ_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR6245248.1"
                     /translation="SIGMYVAKLDRPWLESSFEVQGFYVNNRALIEKLGAECEFVFVD
                     PRRFKEIAEKPRLRVVAHNNVPRPASEGPAPTGRAKLRPVKPRSPAVYEDTVETAAEI
                     GVAQTSIDQAIEIMQPVVERLLATGRLESDQIEAAVTPLVSSVLRNKDAVAALLRIRS
                     LDDYTYSHSISNAVWAAVLGRHLGFSADQINTLSLGCALVDIGKVTLPRILLSKPDPL
                     TEAEEAAFRGHVEAGVEMIRQSTLNDRKVVMMVATHHERIDGSGYPRGLAGDDIPTFG
                     QIAGIIDTYDAMITERRYAPAMSSYEAVYQLQTYAGIKFQEELVEQLTQAIGVFPTGT
                     LVELNTGEVGVVIAQNPERRLRPKINLILNEEKNLRRLPIITDLNAMDTDPATPPRVW
                     IKRELPHGAYGIEATDYFI"
BASE COUNT         1063 a         2004 c         2136 g         1171 t
ORIGIN      
        1 gatttcttcg ccgggctggg ttatcaggtc ctcacccatg gccagaaggc atggaacggc
       61 gtcgcggtgc tcagccgcct gcccgtggaa atcgtccaga agggactgcc aggccaggag
      121 tcggcaggat cccgcctgat caccgtgcgg gtcgacgatc gcctggagtt cacgaccgtc
      181 tactgcccca acggcaagac gctcgaccac gaggactacg cgaagaagct tgcctggttc
      241 acctcgctca gcgagtactg gcgtgatcgc ccctcggcag gggccgtcct ctgcggagac
      301 ttcaacgtgg tgccggaagc cgtcgacagc tggcggggcg aagccggcga cggcaggatt
      361 tttcacacgc tcgaagagcg tgcggccttc cggtcgctgc tcgatgcggg gcttgaggac
      421 ctgtttcgta tggccaagcc ggacgagcag gcgttttcgt ggtgggacta ccgcggcggg
      481 tcgtttcacc gcggccacgg gctgagaatc gacctgatac tgggctcgcg cgacgtggcc
      541 gagcgcgtgg tcgacgtccg taccgatcgg gactatcgca agaagaagga cggactgacc
      601 gcctcggatc acgctcccgt catcgccgag atcgaccttt aaaagattgg tcgccccgct
      661 ttcgggactg gcccagaggc cgcaggctgg acaccgcttg gttaaatggt tcacggtttg
      721 agggcccgaa aggggatatc gtctcccagc aggcagacct gggatcaggc ttaccggcag
      781 atttattggc ggatgcacgg aatgcttcca gagtggttca gatcagacga gatcttctcc
      841 gacgaggggc ggtggtactt cggtgcccgg ggcgggttgc acgtcggtcc ttatggggac
      901 cgcgagctcg ccagcgcccg cagcctctcc gtctcgcgcc gcttacgggc gctcagaacc
      961 gacgccgatc ggctcaggta cgtccgcaag gtgctccacg aggagtggga gagcctgggg
     1021 gtatccgcca gcgctgcgga ggcggaaacg accatcgatc tctctcctcc cagcgagccc
     1081 gtccgtagcg gtgagcgcca gcggaagtgg tgccgttcga gccgtttctt ccagatccac
     1141 ggcgtgtggt tcgtgtccac ccgggagggc atcaacatcg ggccctacgg aacgcagttc
     1201 gaggccagga agcacgagcg ccacctcatc gcgcttctcg tcagggcgag agatcccgcg
     1261 gaggccagac gggtgatcta tcgcttcaag caccgaccaa acctgacggg tactccctgg
     1321 tctcaaaccg acgaggtagc ccgcaccggc tgaacgcggc ttcacggcgg cagctctcaa
     1381 gtcgatgcga gccgctcccg cttgatccgc ttggccagag accagcggtg aacctcggag
     1441 gggccatcgt agatccgaaa ggctctgatc tccctgaaaa cctgctccac gatggtgtcc
     1501 tgggtcaccc cggtgccgcc cagcacctgc acgcagcggt cggcgacacg gaacagcgcc
     1561 tcggagacgg cgaccttcgc catgctcgac tcggcggtgc ccggcgcgcc cgagtcgagc
     1621 accccggcgc accagtcgat catcagctcg gcctgtttca ggtccatcag gttgtcggcc
     1681 agcatgaagc cgacaccctc gtggtcgatg attggcttgc cgaaggcctg gcgccggcag
     1741 gcgtagtccg aggcgatgtc gtgggcccgc tgggcgctcc cccaccagcg catgcagtgg
     1801 gtcagacgcg cgggcgccag gcgcacctgg gcatagcgga agccctcgtc cagctcgccc
     1861 aggatctggt ccgccggaac ccgcaccccg tcgaggcgca cgacggcgtg gcccccgggc
     1921 atggagctgt cgatggtgtc caggacgcgc tcgatggaga tgcccggcgt gttcatgtcg
     1981 gcgagaaaca tggttgccga cgacccggtc ttggccatga tgatgccgaa cccggcgcct
     2041 tcgccgccgg tgatgaacga cttgcggccg ttgatcaccc agtcgtcgcc ctccctctcc
     2101 gccatggtgg ccagcatgcc cgggtctgac cccgcgccgc cgtcgggctc ggtcatcagg
     2161 aacgcggacc ggatggtgcc cgccgccaga ggttgcagga accgctcctg ctgctcgggg
     2221 gttgccacct tgtgcagcag gtgcatgttg ccctcgtcgg gggccatgat gttcagcgcg
     2281 atgggcccca gcggcgagta cccggcggcc cggaacacgg cagccatctc ccggtggttg
     2341 aggtcatggc cgccgaggtg ctcggccaac tggggcacga ggaggccggc ctcacgagcc
     2401 gccgtctgca gttcgactcg cagagcatcg ctgggcccgt gtgatccgtt gcgaggatcc
     2461 ttctcgtagg gaatgacttt gtcggtgacg aactcggtgg ttcgcgcgac cagttcagaa
     2521 actttggggc tcaacgcggt gtccggctgg ttcattcgag gatgtcctga agtttgtaga
     2581 cgttgatggc gttgtcgcgg agaaatgccg gccaggtatc gtctgtcagc ggcaggtcgt
     2641 ccatctccgt gaagatgcgc tcgtagctca ggccggggaa atagccggcg tagagcacct
     2701 tgccgcggcc gcgcttgttg gcgaactcga gaacgttcct ggggtaatgc ttaggcgccc
     2761 aggcagacgt ggaatagtag agattcggcc acttcaacag cagcttcacg caaaggtccg
     2821 tccatgggtc cccgccgtgc cgcatgacca ccttcagctc cgggaaaaac caggccacct
     2881 cgtcgagcag gcccggatgc tggcacctgt agggaacgcg tggtcccggc acgccgacca
     2941 gcatgttgat gggaatgtcg agctcgcagc acttcgcgta gatgggatag aacttcttgt
     3001 cgtcgatggg cacctgagga ttcagcaggc agggcgcggc ggaggcggcc ttgatgttgg
     3061 ggtgcagccc gacgcaggtc tcgagcgcgc gaacaccctc catacccgag ttgggatcga
     3121 cgcctacctc gccgaagaat cggccgggat gcttctcgaa aagatccagg gcggcctcgg
     3181 gtttatgagg gttgacggcg atctgcgcca tggccacgcc gaaatcgtcc atcatgccca
     3241 ccaccgcgtc gggatcggtg gccaaagaca tgcgttccgg ggcgtccttg aagaggtact
     3301 gggccggatg gtgggagaac tcgcccgttc ccttgtccct tgtcaatgca cgggcctgct
     3361 tcatgcccat gccggcagtg ccggtgggca tctcgagcat caggtcgatc accccaagat
     3421 ctgtcggtcg ccccatccgt gctccccgtc ggctgtctcc aaagcgcgct cagtttaacc
     3481 ggttgccgcg cggcgcgctg ttgcggatga ttgaaggccg caagccatgg ctgggaagcc
     3541 cgcatgatca ggatttatca cgttgccggc accagatcgg tccgggtcat ctggttgtgc
     3601 gaggagctcg ggctgcctta cgagaaggtg gccgtcgact tctctccggc ctaccgggca
     3661 acccccgaat ggcgaaggct caatccggta ggcaaagtgc ccgtgctcgt cgacgacgac
     3721 gtcaccatgt tcgaatccgg tgccatggtc gagcatctgc tcgaccgtca cggagcgggt
     3781 cggctgcgtc ccccgccggg tactgccgag cgtgccgcct atctgcaatg gtcctggttt
     3841 gcggaagcca cctttgcccg gccgctcggc gagatcgtca accatcgccg ggagtttccc
     3901 ggcgaatcag aaattccggc tgtgttggag gagatgagcg cccgtgctgc cctctgtgtt
     3961 caggcgctgg acgaagccct cgccggccgc cagtatctgc ttggcgacga gttcacggcg
     4021 gcggacgtga tgatgggcta ttccgttctg ctggcgcgtg ctctgggaat tgagggggcc
     4081 cgggccggtc gtgtgggcgc atacttcgac gtgctggccc gaaggtccgg atatcctgcg
     4141 gcaacggcgg accttcccga cgcctggcgc tgacgctgcc gcgccccgga atgagcgatg
     4201 aagaaccgga ggaaaagcat gagcagatcg acatttgatg ttcggccgct ggacgcctcg
     4261 ttcggcgccg tggtgaccgg cttgcgcctt gcagaccttg gggatgatgc ttttttcgac
     4321 ctttatgacg catggctgca gcacgcgctg ctcatcttcc ccgatcagca cctgagcaac
     4381 gccgagcagg tccgatttgc gcagcggttc ggagcgctgg agttcgatct cacgccgctg
     4441 agcaacgtgc gcggggacgg cagcctgcgc cccgatgacg gcagcgatga cgtggtcaag
     4501 gtacttcgcg gcaacatggg ctggcattgc gacagcacct acatgcccgt ccaggcaaaa
     4561 ggcgcggtgt tcaccgccca cgtcgtcccg gacgaaggcg gcgagacggg ctgggcggac
     4621 atgcgtgccg cttacgatgc gctggacgag gagatgaggg agcgcattgc cgacctgtgt
     4681 gcctaccact cgctctacca tagccaggcg aaggtgggtc atgtgccaag cagcgaaagc
     4741 gcctacagca actacgggtt tcacgagggc gagccgcctc tgcgccctct cgtgaagatt
     4801 cacccggaga caggccggcc ggctctcatg gtgggccgcc atgcctacgg cattccgggc
     4861 atggatgccg atgcttcaga gcgtctgctt gacgagctcg tcgagtttgc ctgccgcccg
     4921 ccgcgcatct atcaccacac gtggtcgccg ggtgatgcgg tggtgtggga caaccgctgt
     4981 ctgatgcatc gggcgtgtcc gtgggacatg agcaagccgc gggtgatgta tcacgcccgt
     5041 attgccggcg accctgcctc ggagtccgga ctggccgcgt gagcggtcgc tctgcccccc
     5101 gctcgcaaga tcggcgtcaa gcgcatcgag acctgccctc agatgaagta gtcggtggct
     5161 tcgattccgt acgcgccgtg aggcaactcc cgcttgatcc agaccctggg gggcgtggcg
     5221 ggatcggtgt ccatggcgtt gaggtcggtg atgatgggca ggcggcgcag gttcttctcc
     5281 tcgttcagaa tgaggttgat cttgggtcgc agacgccgct cggggttctg cgcgatgacc
     5341 acgcccactt cgcccgtgtt gagctccacg agcgtgcccg tgggaaacac gccgatggcc
     5401 tgggtcagct gctcgacgag ctcctcctgg aatttgatgc cggcatacgt ctgcagctga
     5461 tagacggcct cgtaggagga catggccgga gcgtaacggc gttcggtgat catcgcgtcg
     5521 taggtgtcga tgatcccggc gatctggccg aaggtcggga tgtcgtcacc ggcgagcccc
     5581 cggggatagc ctgacccgtc gatcctctcg tgatgagtgg ccaccatcat gacgaccttt
     5641 cggtcgttca aggtggattg ccggatcatc tcgacgccgg cctccacatg cccgcggaag
     5701 gctgcctctt ccgcctcggt cagcgggtcc ggtttggaaa gcaggatcct cggcagggtg
     5761 accttgccga tgtcgaccag cgcgcagccc agggacaggg tgttgatctg atccgccgag
     5821 aacccgagat gccgacccag cacggcggcc cagacggcat tggagatgga gtgagagtag
     5881 gtgtagtcgt cgaggctgcg gatgcgcagc agggcggcca ccgcgtcctt gttgcgcaac
     5941 acgctggaga ccagcggtgt gaccgctgcc tcgatctggt ccgactcgag ccggccggtc
     6001 gccaggagcc gctcgaccac gggttgcatg atctctatgg cctggtcgat tgaagtctgg
     6061 gcgacgccga tctccgcggc ggtctccacg gtgtcctcgt aaacggccgg cgatcgcggt
     6121 ttgactggtc tcagcttggc gcgccccgtc ggggccggcc cttccgacgc cgggcgcggg
     6181 acgttgttgt gggccaccac ccgcaaccgg ggcttctccg cgatctcctt gaaccgtctg
     6241 gggtcgacga atacgaactc gcactcggcg ccgagctttt cgatcagcgc gcggttgttc
     6301 acgtagaagc cctgcacctc gaacgaggac tccagccacg ggcgatcgag cttggctacg
     6361 tacatgccaa ttga
//
LOCUS       DBNHOX010000039         6699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1417372, whole genome shotgun sequence.
ACCESSION   DBNHOX010000039 DBNHOX010000000
VERSION     DBNHOX010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1417372"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..355
                     /locus_tag="AC16UZ_00830"
     CDS             20..355
                     /locus_tag="AC16UZ_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245249.1"
                     /translation="MTTTREYVGGDRYQFDFGRFHYKHGWAQVDTGQDASYFGVWAHP
                     GSFTIATFCEGDLTVQTTESWREFVAEIHRIREWNIEQGHEFKGIDGMCDDAIIGAFA
                     DLGLSDLMH"
     gene            403..558
                     /locus_tag="AC16UZ_00835"
     CDS             403..558
                     /locus_tag="AC16UZ_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245250.1"
                     /translation="MAKTYLLRDIPDDLWPAARARAKSERRTMASAIFDLLRYYVQHG
                     MPWETRS"
     gene            555..992
                     /locus_tag="AC16UZ_00840"
     CDS             555..992
                     /locus_tag="AC16UZ_00840"
                     /inference="COORDINATES: protein motif:HMM:NF016167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR6245251.1"
                     /translation="MTDDKTITLASLAAEPGRQSKTENFGEADDLTGRQLFYTETTTT
                     PAAGTPVQVVANLPPVTRTPGVCGGEACVPGTRIPVWLLVTVRRRFGRETLSDWYPHV
                     SQDRLDAALRWADDHPVEIDAAIDRVVTWNARRATCRSGTAAE"
     gene            1089..1733
                     /locus_tag="AC16UZ_00845"
     CDS             1089..1733
                     /locus_tag="AC16UZ_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245252.1"
                     /translation="MTTIPQDPPDASSVARAKHYQVSQQAEFFAKLRLDQLEDPNAAD
                     TTEADKADVREAAERFVVETERWGPLAGQRGKAELAGAAVLRMRLRVDLEDLTPEQVM
                     ERLVGHFRAALNAYRRQLTIFEWVPDVDRSQSWYVLKDEAGAFFLCPACREARGDTLP
                     KLMRGRSISAPGPVTLRRIDDPTEVAELAEKDLCHDCGRFSESTPETMLDREVE"
     gene            1734..1946
                     /locus_tag="AC16UZ_00850"
     CDS             1734..1946
                     /locus_tag="AC16UZ_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245253.1"
                     /translation="MAKRRTIADAERIITEQIEKYEAGRAATLQARQQLENELYEWDA
                     QLAALKQTLKLIQDQPGGEDDTEETS"
     gene            1943..2641
                     /locus_tag="AC16UZ_00855"
     CDS             1943..2641
                     /locus_tag="AC16UZ_00855"
                     /inference="COORDINATES: protein motif:HMM:NF016302.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HIR6245254.1"
                     /translation="MTDETHEPERPPLGQLGLALAKAQGELGKVTKSHTANAGKYSYT
                     YADLASVIEAIREPFAKHELAYFQAPSADGERVTVTTTLIHSSGESLSSALTLVAADD
                     SPQAIGSAITYARRYGLLAAAGLAPEDDDGAAAQPSNEPETRRSMQNRSKRTDDRTIS
                     EAQAKRLYAMWKSAGRDDNDVKAYLSATYNINSTREIKRADYEAICAWVTASDEQEQA
                     EVDADELTADDISF"
     gene            2646..3068
                     /locus_tag="AC16UZ_00860"
     CDS             2646..3068
                     /locus_tag="AC16UZ_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NinB/YbcN family protein"
                     /protein_id="HIR6245255.1"
                     /translation="MEDYRAAARLEGSKLTLLAPNAYREAMRRLPDGDYVVSVEPATV
                     RRRSLAANRFYWLLLTAIAEQTGHDKEDLHAYFRKRFLTEGFALVNTDGEVIDDREVS
                     KSTTTLSPEAFNLYVMQVEQVASETLGCEVLPEREGVA"
     gene            3065..4012
                     /locus_tag="AC16UZ_00865"
     CDS             3065..4012
                     /locus_tag="AC16UZ_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245256.1"
                     /translation="MSDDLDQYGPVQWPRNASDAIRVYQDAYQMAVRIRDTYGDETIG
                     PAGGFFHKRADRVDYVNLLQESRVYFWERDQAELVSSSARSYPLRAEAVRSLEAPGDE
                     PPSWFPRGMPWAFCVFRSPILGATYNGVWRPFTALLWAIGVGGRDHEIRLAIAGFEWR
                     PRVTVPIWWSDTSDQFGHSDRALIQHSTLSEYQAEREEFIKWICTASTFVEQRIMAHD
                     EVKPSRRLAKQSSGRAAPLPVCRVVRLRRVATGDREQHKMTTGQHEHAYRWWVRGHWR
                     NQWFPSRKMHAPMWIAPHLKGPDDKPVRPIVPTVHHVAQ"
     gene            4024..4353
                     /locus_tag="AC16UZ_00870"
     CDS             4024..4353
                     /locus_tag="AC16UZ_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245257.1"
                     /translation="MSMDTRDLAHPKPRPAALDRSDRKRARARHDEQESDRVRARAGG
                     RCESCGRQGPGHVHHLRGGHGVRGRGESASAAWKVFVCAACHEAIHRHVLHVEGQPGQ
                     WTFRREQ"
     gene            4353..4694
                     /locus_tag="AC16UZ_00875"
     CDS             4353..4694
                     /locus_tag="AC16UZ_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245258.1"
                     /translation="MKIWEDRERDDLQQALLIVKRQRSNYIEETRQLREQVKTLQADY
                     DAWKIAERGLWAEIERLASLAEARLAVVRLVEWNTAPSDVACPVCKGEPRSGHAGDCA
                     IAAVLPDTDVD"
     gene            4722..5537
                     /locus_tag="AC16UZ_00880"
     CDS             4722..5537
                     /locus_tag="AC16UZ_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245259.1"
                     /translation="MTGYTKLFSSLVTSTVWREPSDLRVVWITLLALADKNGVVEGSI
                     PGLADMARVEVETCRAVLQRLQEPDEDSRTQEFDGRRIEPVDGGFRILNYAKYRAKMS
                     ADERRDYQREKQREYRQRRKRGVDTVKNAGNVSGTRAQGRHIAEAEAEAEADTEAKAR
                     TARARASETFSVSDNTLHAWISRWETRWPGEIARVTPAQIATLRGHAKHYGHDELVAR
                     MDRYFASKDRWLVEHKHPLGRFVNGIAQYGEPEPVVSPMDQALANARKKRGLA"
     gene            5534..6184
                     /locus_tag="AC16UZ_00885"
     CDS             5534..6184
                     /locus_tag="AC16UZ_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245260.1"
                     /translation="MTDQDFDEFAELFGQVAVALPMPSGQASDPTVLERKADVYFEAL
                     RDLPLSAVRASTRTLLRSGSGFFPSTQEWYQQADTIAFDALVSSRPTFPAGTSRPTFP
                     TDTDQSAFEKVRADREAFLAALRAKPNKRPWINYQAVADVVERVAQRRTPVRPTTWCD
                     DCQDVGMVKRVCRHLERCAAHTDVDEDWGEHDHVTRCSCVPANPVIQRRQELRRRA"
     gene            6181..6303
                     /locus_tag="AC16UZ_00890"
     CDS             6181..6303
                     /locus_tag="AC16UZ_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245261.1"
                     /translation="MTTFDRVTYMAIGLLVLVSAVSAACVPILAVAALLKWILS"
     gene            6469..6693
                     /locus_tag="AC16UZ_00895"
     CDS             6469..6693
                     /locus_tag="AC16UZ_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245262.1"
                     /translation="MTTHFRGTDYSETASKERIYLGHETAPDSSWWAEAPRDGFTDYA
                     RAVRAKRETTSHGRKRGPEFNLAMDRPIGE"
BASE COUNT         1364 a         2021 c         2254 g         1060 t
ORIGIN      
        1 cagacgaaag gacgaaccca tgaccacgac aagagagtac gtcggcggag accgatacca
       61 gttcgatttt gggcgattcc actacaagca cgggtgggcg caggtggaca cagggcaaga
      121 tgcctcgtat ttcggggtat gggcgcaccc gggctcattc acgatcgcga cgttctgcga
      181 gggcgacctc acggtgcaga cgaccgagtc ctggcgtgag tttgtcgccg aaatacaccg
      241 gatcagggaa tggaacatcg agcagggcca tgagttcaag gggattgacg gcatgtgcga
      301 cgacgccatc atcggcgcat tcgctgacct cggactatct gacctgatgc actaacacac
      361 ccttgacgcg cgttatcaac gcgcgtatcg tagacatact acatggctaa gacataccta
      421 ctccgcgaca ttcccgacga cctctggccg gccgccaggg ccagggccaa atcagagcgg
      481 cgcacgatgg ccagcgcgat ctttgacctc ctgcgctact acgtccagca cgggatgccc
      541 tgggagaccc gatcatgaca gacgacaaga cgatcacact cgcgagcctg gcggcagagc
      601 ctggccggca gtcgaagacg gagaacttcg gcgaggccga cgacctgacc ggacggcagc
      661 tcttttacac ggaaacgacg acgacgccgg cggcgggaac gcccgtgcag gtggtggcga
      721 acctgccgcc cgtgacacgc acgcctggcg tctgcggcgg ggaagcttgt gtgccaggga
      781 cacggattcc ggtgtggctg ctcgtcaccg tgcggcgcag gttcggacga gagaccctat
      841 ccgactggta tccgcacgtg tcgcaggacc gactcgacgc cgccctgcgc tgggctgacg
      901 accacccggt cgagatcgac gcagcgatcg acagggtagt cacatggaac gcgcgccggg
      961 caacgtgccg ctcaggaact gctgcggagt agacgcagcg attgccagaa cgacaatgaa
     1021 ccagacgtaa aaacggccct gcgcgccgtg aaacacgcag ggccgaggca ccacgaaagg
     1081 aacgaaccat gaccacgata ccacaggacc cacctgacgc gtcaagcgtc gccagagcca
     1141 agcactacca ggtcagccag caggccgagt tcttcgcgaa gctccgactc gaccagctcg
     1201 aggaccccaa cgccgccgat acgaccgagg ccgataaggc cgacgtgcga gaagcggccg
     1261 agcggttcgt cgtcgagacc gagcgctggg gccctctggc cggacagcgg ggcaaggccg
     1321 aactggccgg ggctgctgtg ctccgtatgc ggctccgtgt cgacctggag gacctgaccc
     1381 ccgaacaggt catggagcgg cttgtagggc acttcagggc ggccctgaac gcctacagac
     1441 ggcaactgac gatcttcgag tgggtgcccg acgtggaccg gtcgcagtcc tggtatgtcc
     1501 tgaaggacga agccggggca tttttcctgt gcccggcctg ccgagaggcc cgcggcgaca
     1561 ccctgccgaa gctcatgcgg gggcggtcga tctcagcccc gggacccgtc accctgcggc
     1621 gcattgacga cccgacagag gtcgccgaac tggccgagaa ggacctgtgt cacgattgcg
     1681 ggcggttctc agaaagcacg cccgaaacca tgctagatcg agaggtggag taaatggcca
     1741 agcgacgcac gattgcagac gcagagcgga ttatcacgga acagatcgag aagtacgagg
     1801 ccgggcgggc tgcgaccctt caggccaggc aacagcttga gaatgagctc tacgagtggg
     1861 acgcgcaact cgccgcgcta aagcagacgc taaagctcat tcaggatcag ccaggcggag
     1921 aagacgacac cgaggagacg tcatgaccga cgagacgcac gaaccagaac gacccccact
     1981 cggccaactg ggtctggccc tagccaaagc gcaaggcgag ctcgggaagg tcacgaaaag
     2041 ccacacggcc aacgccggca aatactccta cacctatgcc gacctggcct ccgtgatcga
     2101 ggccattcgg gagccgttcg cgaaacacga actggcgtat ttccaggcgc cctcggcaga
     2161 cggggagcgg gtcaccgtga cgacgacgct gatccacagt tcaggggagt ccctgtcctc
     2221 ggccctgacc ctggtggcgg cggatgattc ccctcaggcg attgggtcgg cgatcaccta
     2281 tgctcggcgc tacgggctcc tggcggcggc tggattggcc ccagaagacg acgacggggc
     2341 ggcggcgcag ccgtccaacg aaccggagac gcgccgatcc atgcagaacc gcagcaagcg
     2401 gacggatgac cggacgatct ccgaggcgca ggccaaacgg ctctacgcca tgtggaagag
     2461 cgccggccgc gacgacaacg acgtgaaggc gtatctgtct gcgacctaca acatcaactc
     2521 gacccgcgag atcaaacgcg ctgattatga ggcgatctgc gcgtgggtga cggcgtcaga
     2581 cgaacaggaa caggccgaag tcgacgccga cgagctgacc gcggacgata tttcattctg
     2641 aggccatgga ggactaccgc gccgccgcac ggctcgaagg ctcgaagctg accctactgg
     2701 ccccgaatgc ctaccgggag gccatgcggc ggctgccaga cggggactac gtggtgtcgg
     2761 tggagccggc caccgtgcgg cggcgcagcc tggcagcgaa ccggttttat tggctgctcc
     2821 tgaccgcgat cgccgagcag accgggcacg acaaagaaga cctccacgcg tatttccgga
     2881 agcggtttct cacggaaggc ttcgcgctgg tgaatacgga cggcgaagtg atcgacgacc
     2941 gagaggtcag caagagcacg accacgttgt cgcccgaggc gttcaacctc tacgtgatgc
     3001 aggtcgagca ggtggcgtcg gagacgctgg ggtgtgaggt gctgccagag cgggaggggg
     3061 tggcatgagc gacgatctcg atcaatacgg gcctgtgcag tggccgcgaa atgcgagcga
     3121 cgcgattcgc gtatatcagg acgcgtacca gatggccgtt cgcatacgcg acacctatgg
     3181 cgacgagacg atcggtccgg cgggggggtt ttttcataag cgcgccgaca gggtcgatta
     3241 tgtcaatctt ctccaggagt cgcgtgtgta tttttgggaa cgcgaccagg ccgagcttgt
     3301 gtcctcgtct gcgcggagtt acccgttgcg ggctgaagcg gtgcggtcgc tcgaagcgcc
     3361 aggcgatgag ccgccgagtt ggttcccgcg cgggatgccg tgggcgtttt gcgtgtttcg
     3421 atcacccatc ctgggggcga cgtataacgg cgtctggcgc ccattcacag cgttgttgtg
     3481 ggccattgga gtaggggggc gcgaccatga aatcaggctc gccatcgctg ggtttgaatg
     3541 gcgaccacgt gtaaccgtcc cgatctggtg gagcgacacg tccgaccaat ttggacatag
     3601 tgaccgggcg ttgattcaac acagcactct gtctgagtac caggcagagc gcgaggagtt
     3661 tatcaagtgg atctgcaccg cgagcacctt cgttgaacaa cgaatcatgg cgcacgacga
     3721 agtgaaaccg tcgcgcaggc ttgcgaagca atcgtctggg cgggcagcgc cgctaccagt
     3781 gtgtcgcgtc gtgcgcctga ggcgagtcgc gaccggcgat cgtgaacagc ataagatgac
     3841 gaccgggcag catgagcatg cgtataggtg gtgggtccgt ggccattgga gaaatcagtg
     3901 gtttccttcg aggaagatgc atgctccgat gtggattgcc ccgcatctga aggggcctga
     3961 cgataagccg gtgcggccga tcgtcccgac agttcatcac gtggcgcagt agggactcta
     4021 gtcatgagca tggacacccg cgacctcgcc caccccaagc cccgcccggc ggccctggac
     4081 cggtccgacc gcaagcgcgc tcgcgcgcga cacgacgagc aggagagcga ccgggtgcga
     4141 gcgcgagccg ggggacgctg cgagtcctgc ggccggcagg ggcctggcca cgtccaccat
     4201 ctccggggcg gacacggcgt cagggggcgc ggagagagcg cgtcagcggc ctggaaggtg
     4261 tttgtctgcg cggcctgcca tgaggctatt caccggcacg tgcttcacgt ggaggggcag
     4321 cctggacagt ggacgtttcg gagggaacag tgatgaagat atgggaggac cgtgagcgag
     4381 acgacctaca gcaagcgctg ctcattgtga aacggcagcg gtcgaactat atcgaagaga
     4441 cgcgccagct ccgagagcag gtcaagaccc tccaggccga ttatgacgcg tggaagatag
     4501 ccgagcgcgg tctgtgggcc gagatcgaac gcttagcgag tctcgccgag gcccgcctgg
     4561 ccgtggtgcg actagttgaa tggaacacgg ctccatccga tgttgcctgc cctgtctgta
     4621 agggtgagcc gcgtagcggc catgccggcg actgcgccat cgctgccgtc ctgccagaca
     4681 ccgacgttga ttagtcatgt agaatcgtgt agagtgtcac catgaccgga tacacaaagc
     4741 ttttttcctc tctcgtcacg tcaacggtct ggcgcgagcc gtccgacctg cgcgtggtct
     4801 ggattaccct gctcgccctg gccgataaga atggcgtggt ggaggggtcg attcctggat
     4861 tggccgacat ggcccgggtc gaggtggaga cctgtcgggc agtcctccag cggttgcagg
     4921 aaccagacga agactcacgg actcaggagt ttgacggccg acgaattgag ccggtcgacg
     4981 gcggattccg gattctgaat tacgccaaat accgggccaa gatgtcggcc gacgaacggc
     5041 gcgactatca gcgcgagaag cagcgcgagt atcgacagag gcgtaaacgc ggcgtcgaca
     5101 ctgtcaaaaa cgccggtaac gtgtcaggga cacgtgcaca aggtcgacac atagcagaag
     5161 cagaagcaga agcagaagca gatacagagg caaaagcacg tacagcgcgc gcgcgtgctt
     5221 ctgagacgtt ctcggtatca gacaacacgc tgcacgcgtg gatcagtcgg tgggagacac
     5281 ggtggcctgg cgagattgcc cgcgtgacac cggcgcagat tgccacgctc cgcgggcatg
     5341 cgaagcacta cggccacgac gagctggtgg cgcgtatgga ccgctacttc gcgtcgaagg
     5401 accggtggct ggtggagcac aagcaccccc tgggccggtt cgtcaacggg atcgcgcaat
     5461 atggggagcc tgagccggtt gtgtccccga tggaccaggc gctggcgaat gcgcggaaaa
     5521 agcgagggct ggcatgactg accaggattt cgacgaattc gcagagctgt tcggacaggt
     5581 ggccgtggca ctgcccatgc ccagcgggca ggcgtcagac cccaccgtgc tcgagcgcaa
     5641 ggccgacgtc tacttcgagg ccctgcgcga tctgccgctg tcggccgtgc gggcgtccac
     5701 acgcacgctc ctgcggtcgg ggtcggggtt cttcccgtcc acccaggagt ggtatcagca
     5761 ggccgatacg atcgccttcg acgccctggt gagctccagg ccgacgttcc ctgctggcac
     5821 gtccaggccg acgttcccta ctgacacgga ccagtcagct ttcgagaagg tgcgtgcgga
     5881 ccgtgaggca ttcctggccg cgctgcgcgc gaagcccaac aagcgcccgt ggatcaacta
     5941 ccaggccgtg gccgacgtgg tcgagcgtgt ggcgcagcgg cgcacgccag tgcggccgac
     6001 cacgtggtgc gacgactgcc aggacgtggg catggtcaag cgcgtgtgcc ggcacctgga
     6061 gcgctgcgcc gctcacacgg acgtggacga ggactggggc gagcacgacc acgtgacgcg
     6121 gtgctcgtgt gtgcccgcga atccggtcat tcagcggcgg caagagctcc ggaggcgcgc
     6181 atgacgacgt tcgaccgagt cacctatatg gccattggcc tgctggttct cgtctcggcg
     6241 gtctcagccg cgtgtgtgcc gatcctggcc gtggcggcgt tgctgaaatg gatcctgtca
     6301 tagccatgaa gctctatgcg gtcatgggac cagtcacccc agcgaggagg cgcgcatgac
     6361 caaccgacgc accgacatag ccattgtcct gctgtttctc gtctcggcgg tctccgccgc
     6421 gtgcgtgacg gtcctggccg tggaggcgtt actgaaatgg atcctgtcat gaccacgcat
     6481 ttcagaggca ccgactacag cgagaccgcg tccaaggagc ggatctacct cggccacgag
     6541 acggcccccg actcgtcgtg gtgggcagag gcaccgcggg acggattcac ggactacgcc
     6601 agggccgtgc gggcgaagcg tgagaccacg tcacacgggc gcaagcgggg tcctgagttc
     6661 aatttggcca tggaccggcc gatcggagag tagccatga
//
LOCUS       DBNHOX010000040         5277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_324942, whole genome shotgun sequence.
ACCESSION   DBNHOX010000040 DBNHOX010000000
VERSION     DBNHOX010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_324942"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="AC16UZ_00900"
     CDS             <1..940
                     /locus_tag="AC16UZ_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR6245263.1"
                     /translation="ASVTENFTGAIADSINFAIDQMRGLVSNINNLSRQVAKAAGDSQ
                     ETASSLAGAAESQAREIAAASQAINEMAVSIDHVSSNAAESASVAERAVGIANKGSDV
                     VQDTIHGMDVIRGQIQETSKRIKRLGESSQEIGDIVSLINDIADQTNILSLNAAIQAS
                     MAGDAGRGFAVVADEVQRLAERSSAATKQIAALVKTIQTDTNGAVISMEQTTAEVVNG
                     AKRAQDAGVALEEIESVSTSLAELIQNISNAARQQAASAGHVSNTMNVIQEITAQTVD
                     STEQTATSIGNLAAMANEMRESVEGFKLPAQYQSQD"
     gene            950..>5277
                     /locus_tag="AC16UZ_00905"
     CDS             950..>5277
                     /locus_tag="AC16UZ_00905"
                     /inference="COORDINATES: protein motif:HMM:NF053188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR6245264.1"
                     /translation="MATSRENFALDWVKGALQESLDEAIAALDSFAEAGHGEAQMREC
                     MTALHQVHGALVMLELKGTTLLAEHLERLAQHLMIGAVGDSVGAGQCLMQGLLELPGY
                     LDEVQRGLSDAIEPMIPLINEVRGHLDERPLEGAMVTDLGVASEAAIERFDGIDGVEK
                     AGRIRAAYQQVLLCILRGDDRERAVITLRKIAEVLSRVCLGTPHETQWRALGEFAASL
                     ATDTGPIDPDAAKLLRRIDSEIRTLSRDGLGVAGSPVSLDLIKQLLDACRQRGYRSEA
                     ITDISNALAGRGDGGGLAMNGLKALVSASLVVREELALVKDQLDLFARARGRASVDLN
                     SLLAPLKQIRSTLTLLGFASAHEIVTQQLEALEQLASAEAVDPQDLFSVASALVRVDE
                     SMASFTEGDQEVEKITNDAQRTVTVEVRNGLEPVRQAVLEYIAAGWDSEPLAGVPEQI
                     AALCGALEMIPLRRAARILSSCGAYLRRDLLEAGATPGMDAMETLADAITAVDYYLQR
                     TAEDITGGIEDILDVADASLEKLGYAGIVDERPAAQPGAAFQAETAGVEETAEEGFDW
                     EGFVSVAADLREEPASSDAQDQEAPEAEPELIDFEPVALEGGSDDEPEAAQAASADAE
                     PEAEPEDASAVEETPVAASAPSPALPPLSPAIAEKTSRLADAFESDEEIVEIFVEEVD
                     EVLETINGCLPRWGEHLSDEDSLTELRRAFHTLKGSGRMVGANIIGELAWSVENMLNR
                     LIDGTFEPTGQFALVVEEATSLMPYLRDAFEAQRAPDLDAMGRIMEKADVLASGGSLE
                     EQAPVAEEEPAVEAAEAGEGFEALGLFVSESEEHLAVLALCDDGEDFVLDEDAYRALH
                     TLAGSAGMAGIEVVSELARPTYEVALAARSEQEVTRASGAVAEFLRAATTALACMIET
                     LRAGSEPEEELQLIADADRLLVEIQETGESKLDELMKLPALAVVMDAEEFLGGWARGV
                     MDLEFAGKLIGALGEIADLARATERSAVADLAAALRATHEELEQETISPESHVALSRA
                     HETLLQQFDAMAAQQTVALATAEIASLTSLREAVTEPSSVEAFGTGAGSSAGSDYAEG
                     AGSRGAEDVAAGDTGTPAEPPAEAGPRVASLPIASLLPEEVDEDIIGIFFEEAEEILE
                     SLDNSIHEWTGEPENRLFLENLLRGLHTLKGGARLSGLSVLGDYTHEFETFLIDFQLS
                     SAPLPEGFFTDLGARYDELEILVSRIRKAIESGERGESFAEAPASATSTPPAEPVAEP
                     ERVAEEDTTGAAAPTDEQPQPAEEAPAQASEVPDLAAAEASAEEAEAEELPAQEIAAS
                     EETDEAEPEPVAEPEPVAEPEPVAAPAPVLRVAPEPSEEAPSAPDPTRADRAKEMVRV
                     GAGLLEELVNLAGESSIVRARIEQGMSDFTGALDEMETTIERVREQLRRLEIETEAQI
                     LFRRDTL"
BASE COUNT         1000 a         1661 c         1817 g          799 t
ORIGIN      
        1 ggcctcggtg acggagaact tcaccggcgc gatagccgat tcgatcaact tcgccatcga
       61 ccagatgcgc gggctcgtat ccaacatcaa caacctgtcg cgtcaggttg ctaaagccgc
      121 gggcgacagc caggagacgg catccagcct cgctggcgca gccgagagcc aggcacgaga
      181 gattgccgcc gcgtcccagg cgatcaacga gatggccgtc tccatcgatc acgtctcctc
      241 gaacgccgcc gagtctgcat cggttgcaga gcgggccgtg ggcatcgcca acaaaggatc
      301 ggacgtggtc caggacacca tccacggcat ggacgtgatt cggggccaga tccaggaaac
      361 gtcgaagcgg atcaagcgcc tcggcgagtc cagccaggag atcggcgaca tcgtatctct
      421 catcaacgac atcgcggacc agacgaacat tctctccctc aacgccgcca ttcaggcctc
      481 catggccggt gatgccggtc gaggatttgc cgttgtggcc gacgaggtgc agcggctggc
      541 tgaacgatcc agcgcggcaa ccaagcagat cgcggctctg gtgaagacca ttcagacgga
      601 caccaacggg gccgtaatct ccatggagca gaccaccgca gaggtcgtca acggtgccaa
      661 gcgcgcccag gacgccgggg tggctctcga ggagatcgaa agcgtatcca cgtcgctggc
      721 cgagctgatc cagaacatca gcaacgcggc acgccagcag gctgcctctg cgggccacgt
      781 ttccaataca atgaacgtca ttcaagagat taccgcgcag accgttgaca gcaccgagca
      841 aacggcgact tcgatcggta acctggccgc catggccaac gagatgcgcg aatcggtcga
      901 aggcttcaag ctgccggccc agtaccaaag ccaggattaa gacctcggta tggcgacaag
      961 ccgggaaaat tttgccctcg actgggtaaa gggcgcgctg caggagtctc tggacgaagc
     1021 gatcgcggcg ctagactcgt tcgctgaagc cggtcacggc gaagcgcaga tgcgcgaatg
     1081 catgacggct ctgcaccagg tgcacggcgc tctggtgatg ctggagctga agggcaccac
     1141 gctgctggcc gagcacctgg agcggctggc ccagcacctg atgatcggtg cggtcggcga
     1201 tagcgttggg gccggtcagt gcctcatgca ggggcttctc gagctgcccg ggtatctcga
     1261 cgaggtgcag cgcgggctct ccgacgcgat cgaacccatg atcccgctga tcaacgaggt
     1321 ccgcggtcat ctggacgagc ggccgttgga aggcgcgatg gtcaccgacc tgggcgtcgc
     1381 cagcgaagcg gccatagagc gcttcgatgg gatcgacggc gtagagaagg cggggcggat
     1441 acgggcggct taccagcagg tcctgctctg catcctgcgg ggagacgatc gcgaacgggc
     1501 ggtcatcacc ctgcgaaaga ttgccgaggt gctgtccagg gtatgcctgg gcactcctca
     1561 tgagacccag tggcgcgcgc tcggcgaatt tgccgcgagc ctggccacgg acaccggccc
     1621 catcgacccg gacgcggcaa agctgctgcg ccggatcgac tcggagatcc gcacgctgtc
     1681 cagagacggg ctcggagttg ccggcagccc ggtttctcta gacctgatca agcagcttct
     1741 ggatgcgtgt cgccagcggg ggtatcgcag cgaagcgatc accgacatta gcaatgcgct
     1801 ggcgggccgc ggagacggcg gcgggctggc catgaacggc ttaaaagccc tggtgagcgc
     1861 aagcttggtg gtgcgcgaag agctcgcgct ggtcaaagac cagctggacc tgttcgcgcg
     1921 cgcgcgtggt cgtgccagcg ttgacctgaa ctcgctcttg gcgcccctca agcagatccg
     1981 cagcacgctg acgctgctcg gtttcgcaag tgctcacgag atcgtcacgc agcagctcga
     2041 agcgctcgag cagctggcgt cggccgaagc cgtcgacccc caggacctct tcagcgtcgc
     2101 cagcgcgctc gtacgtgtag acgagagcat ggcgagcttc accgagggcg atcaggaggt
     2161 cgaaaagatc accaacgacg cccagcgcac cgtcaccgtg gaggtgcgta acggcctcga
     2221 gccggtcaga caggccgtgc tcgagtacat cgccgcgggt tgggactcgg aacccctcgc
     2281 cggcgtgccg gagcagattg ccgctttgtg cggcgctctg gagatgattc cgctgcggcg
     2341 cgcggcccgg attctgagca gctgtggcgc atacctgcga cgagacctgc tggaggcggg
     2401 tgccaccccg ggcatggatg ccatggaaac cctggcagac gccatcacgg ccgtcgacta
     2461 ctacctccag cgcacggctg aggacattac tggcgggatc gaggacattc tagacgtcgc
     2521 ggacgcaagc ctcgagaagc tcgggtatgc gggcatcgtc gacgagcgcc cggccgcgca
     2581 gccgggcgca gcgtttcagg ccgagaccgc cggcgtcgaa gagaccgcgg aggaaggctt
     2641 cgactgggag ggctttgtct cagtggctgc cgacctgcgc gaagagcctg cctcgtcgga
     2701 tgctcaggat caggaagcgc ccgaagccga gccggagctc atcgacttcg agcccgttgc
     2761 cctcgagggc ggctcggacg acgagccgga agcagcgcag gctgcttccg ccgacgccga
     2821 gccggaagca gagccggagg acgcctccgc tgtggaagag actccggtgg ccgccagcgc
     2881 gccgtcaccc gcgctgccgc cgctgtcgcc ggccatcgcc gaaaagacca gccggctggc
     2941 cgacgcgttc gaatccgacg aggaaatcgt cgagatattc gtggaggaag tcgacgaagt
     3001 tctggagacc atcaacgggt gtctgcctcg ctggggcgag catctgagcg acgaggactc
     3061 gcttaccgag ctgcgtcgcg cgtttcacac gctcaagggc agcggccgca tggtcggcgc
     3121 caacatcatc ggcgagctgg cctggtcggt ggagaacatg ctcaaccggc tcattgacgg
     3181 caccttcgag cccaccggac agtttgcgct ggtcgttgaa gaagcaacgt cgttgatgcc
     3241 gtatctgcgc gatgcgttcg aagcccagcg ggcccccgat ctcgacgcta tgggccgaat
     3301 catggagaag gccgacgtgc tggcgtcggg cggctccctg gaggagcagg cgccggtcgc
     3361 ggaggaagag ccggccgtgg aagctgcgga agcaggcgag ggcttcgagg cgcttggcct
     3421 gttcgtatcg gaatccgagg agcatctggc cgtgcttgcc ctctgcgacg acggagaaga
     3481 tttcgttctc gacgaggatg cctacagagc gctgcacacc ctggctggca gtgcgggcat
     3541 ggcgggcatt gaggtggtga gcgagctggc ccgtcctacc tatgaggtgg ctctcgccgc
     3601 ccggtctgaa caggaggtca cgcgagcttc aggtgcggtt gccgagttcc ttcgggccgc
     3661 gacgacggcg ctggcctgca tgatagagac gttgagagcc ggctcggagc ccgaggaaga
     3721 gctgcagctc atcgcagatg ccgatcggct gctggtggag atacaggaga ccggagagag
     3781 caagctcgac gagctcatga agctgccggc cctggcagtc gtcatggatg cagaggagtt
     3841 tctgggcggt tgggctcgcg gcgtcatgga cctcgagttc gccggtaagc tgatcggggc
     3901 tctgggcgag atcgctgacc tcgcgcgagc gactgagcgc tcagcggtcg ccgatctggc
     3961 agccgcgctg cgagccactc acgaggagct cgagcaggag acgatcagcc cggagagcca
     4021 tgtggctcta tcgcgcgccc acgagaccct gctccaacag ttcgacgcca tggccgccca
     4081 gcagacggtt gccctggcta ccgcggagat cgcatcgctc acgtcactgc gtgaggcggt
     4141 cacggaaccg tcctcggtgg aagcgttcgg aaccggagcg ggctcttctg ccggcagcga
     4201 ctacgccgag ggtgcaggat cccgcggcgc agaagatgtc gccgcgggcg acaccggcac
     4261 gccggccgag ccgccggcgg aggccggccc acgggtcgcc tccctgccca tcgcttcgct
     4321 gcttcctgaa gaagtcgacg aggacatcat cggtatcttc ttcgaggaag ccgaggagat
     4381 ccttgaatcg ctggacaaca gtattcacga atggacgggt gagcccgaga acaggttgtt
     4441 cctggagaac ctgctgcgtg gccttcacac gctcaagggc ggggcccgtc tttcggggtt
     4501 gtcggtcctc ggtgactaca cccacgagtt cgagaccttc ctcatcgact ttcagttatc
     4561 cagcgcgccg ctgcccgagg gcttcttcac cgatctaggc gcccgttacg acgagctcga
     4621 gatcctggtc agcaggattc gcaaggccat cgaatcgggt gaaaggggcg aatccttcgc
     4681 cgaggcgccg gccagcgcga cgtcgacgcc cccggcagag cccgttgcgg agccggagcg
     4741 tgtggcggaa gaagacacga cgggcgcagc cgctccgaca gacgagcagc cgcagcccgc
     4801 cgaggaggcg ccagcgcagg cgagcgaagt gcccgacctg gcggcggccg aagcatcagc
     4861 cgaggaagca gaggccgagg agcttccggc ccaggaaatc gcggccagcg aggagaccga
     4921 cgaagccgag cccgagccgg tcgcagagcc ggagccggtt gcagagccgg agccggttgc
     4981 tgcgcctgcc cccgtgctgc gggtggcccc ggagccgtcc gaggaagcgc cgagcgcgcc
     5041 cgatcccaca cgggccgacc gggccaagga gatggtccgc gtaggggccg gactgctcga
     5101 agagctcgtg aaccttgccg gcgaaagcag catcgtcagg gcccgcatcg agcagggcat
     5161 gagcgacttc accggcgccc tggacgagat ggaaaccacc atcgagcgcg tgcgcgagca
     5221 gctgcggcgc ctcgagatcg aaaccgaagc gcagatcctg ttccgtcggg acacgct
//
LOCUS       DBNHOX010000041         1847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_582470, whole genome shotgun sequence.
ACCESSION   DBNHOX010000041 DBNHOX010000000
VERSION     DBNHOX010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_582470"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="AC16UZ_00910"
     CDS             <1..259
                     /locus_tag="AC16UZ_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245265.1"
                     /translation="PGGLAILPVPIQAQQTVEYGEARADEHHHVRAPGLDYYDRYRSV
                     FERVDEVASGDFDPRYQLFALEPHEGRVRARRDLVPLCYKE"
     gene            complement(247..879)
                     /locus_tag="AC16UZ_00915"
     CDS             complement(247..879)
                     /locus_tag="AC16UZ_00915"
                     /inference="COORDINATES: protein motif:HMM:NF016087.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE"
                     /protein_id="HIR6245266.1"
                     /translation="MNDVRKLGALLCLLAGCGACGPTGDAPGITAAPDPADDATQTPV
                     DGHSSRDSLDWSGVYSGVTPCADCPGIRTTLTLHGDGSFERTRFYLERSAAPQRQAGQ
                     FLWNASGSAITLQEPEGHGQHYKVAENALIHLDRAGKPITGELAGRYVLSKHRRDPAI
                     EGRRWTLVELQGRPLEADAPGSTPSLLLDPEQSLAAESCEPGLHTANHSL"
     gene            complement(947..1267)
                     /locus_tag="AC16UZ_00920"
     CDS             complement(947..1267)
                     /locus_tag="AC16UZ_00920"
                     /inference="COORDINATES: protein motif:HMM:NF019491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HIR6245267.1"
                     /translation="MSSTNRMIRHIVLVKFREDAPDELRQELVRLSQWGRDADYVSGY
                     VCGWGMQPNLYPGAGEEWDWGMSLDMAEEDAKRYAEDPTHTAIPQEVIDCAEKFAILD
                     FLME"
     gene            1654..>1847
                     /locus_tag="AC16UZ_00925"
     CDS             1654..>1847
                     /locus_tag="AC16UZ_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6245268.1"
                     /translation="MKQTQPIECGIPVVDLDRMLEFYCSVLGCRESRRADIPAQLSQR
                     LSVAPDGYVNVWLETPGGEI"
BASE COUNT          348 a          620 c          563 g          316 t
ORIGIN      
        1 tccgggtggc ctggcgatcc tgcctgtgcc gatacaagcg caacagaccg tcgagtacgg
       61 agaggcccgg gcggacgaac accatcatgt gcgcgcgccc ggcctggatt attacgaccg
      121 atacaggtcg gtgttcgaac gggtcgacga ggtcgcttcc ggcgacttcg acccacgcta
      181 ccagctcttc gcactggagc cccatgaagg tcgggtacgg gcgcggcgag atctggtgcc
      241 actttgctac aaggaatgat tcgcggtgtg aaggccaggc tcgcaagatt cggcagcgag
      301 cgactgctcc ggatcgagca acagggaggg cgtgcttcca ggcgcatccg cctcgagcgg
      361 cctgccctga agctcgacga gcgtccacct cctgccttcg atggccgggt cacggcggtg
      421 cttgctcagc acgtagcgtc ccgccagctc accggtaatc ggcttgccag cgcggtcgag
      481 gtgaatcaac gcgttctctg cgaccttgta gtgctgacca tggccctccg gttcctgcag
      541 ggttatggcg cttcccgatg cgttccacag gaactgcccg gcctgtctct gcggagcggc
      601 gcttcgctct agatagaagc gggttcgctc gaaactgccg tcaccatgca acgtgagcgt
      661 ggtccggatc cccggacagt cggcgcaggg cgtaacgccc gaatagacgc cggaccaatc
      721 cagggaatcg cgactcgaat gcccatcgac gggcgtctgc gtcgcatcgt ctgccgggtc
      781 tggagcggcc gtgattccgg gagcatcccc cgtggggccg cacgcgccgc aaccggccag
      841 tagacacagc agcgcgccga gttttctcac atcattcatc aaccacgtcc gcaaagtcgg
      901 gaagcgatgc cccgctcgtc gaagacccct gaccgagccg gcccgcctat tccatcagga
      961 agtccaggat tgcaaacttc tccgcgcagt cgatcacttc ctggggaatg gccgtgtggg
     1021 tagggtcctc tgcatagcgt ttggcatctt cctccgccat gtccagggac atcccccagt
     1081 cccattcctc accagctccg ggatatagat tgggttgcat gccccacccg cagacgtacc
     1141 cggacacgta gtccgcatcc ctgccccact gcgaaagccg gacgagttcc tggcgcagct
     1201 cgtccggtgc atcttcacga aacttgacca gaacgatgtg acgtatcatt cggttggttg
     1261 agctcatagc tgattctccc ggttagttcc ccacgggccg caaccaacgt ccgcagcgaa
     1321 cgccagtccg gcaatgaccc tcaaggcgag atgatcgccg aggcggcgga ggctgccaac
     1381 accgccgctg cgacgttcgg acctcgacaa cgcttcagac gaaccgccac cgatcgaaaa
     1441 atatactcac gccgccgtgc ttcgtcggga tccgcgcccg ccgccagacg tgttgaggaa
     1501 ccccgggaag ggctttccag ccgagaagcg cgcaggcaga atccatgacg ctctcacacc
     1561 gatctccgat tcgccgggcg gggcaggcca gacaacggcg cgcgctgccg ccgaggcccc
     1621 agaaccggcg gccgccgcca acgaggaaaa ccgatgaagc aaacccaacc catcgaatgc
     1681 ggcattcccg tggtcgacct ggaccggatg ctcgagttct attgcagcgt gctgggctgc
     1741 agagagtccc ggcgcgcgga cattcccgcc cagctgagcc aacggctctc ggtggccccg
     1801 gatggttacg tcaacgtctg gctggagacg cccggcgggg aaatcat
//
LOCUS       DBNHOX010000042         1677 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1160860, whole genome shotgun sequence.
ACCESSION   DBNHOX010000042 DBNHOX010000000
VERSION     DBNHOX010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1160860"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..1107
                     /locus_tag="AC16UZ_00930"
     CDS             28..1107
                     /locus_tag="AC16UZ_00930"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR6245269.1"
                     /translation="MLHLGIDLHQSQITIVLLDEAGDIVRRATVSTRPPFIDEFLASI
                     NALARESGGYRAIVEECGFTHWLIERLKIHECDLTVVVQPLKRARHKTDKRDAATLAE
                     ILWTNRDRLEAGSRLHGIRQVMVPTEQDRQARRLTNAKIRLVRQRTAAINLIKGVLRQ
                     FNLMHDCPTKTFQTKAAKAWLRSIELPKADKMILELALEQWELLDRQIDTLDPEIDRR
                     ADESSAAQVIKTIPGAGSLTALVLASRIGSIDRFPRPSSLANMFGLTPTINDTGDSTG
                     RLGGITKHGHPNVRFLLGQMVAQVTRADPHLRAIHRKIRKRRGRKTAMVATMRRITCR
                     VWHMLKTGEAYRIGPGRATASTPGQ"
BASE COUNT          345 a          547 c          539 g          246 t
ORIGIN      
        1 gcgacgtggc gccgggaggt atcagtgatg ctacatctag gaatcgacct gcaccagtct
       61 cagatcacga tcgtcctgct cgacgaggcc ggcgacatcg tccgtcgggc cacggtctcg
      121 accaggccgc cgttcatcga cgagttcctg gcgtcgatca acgccctggc cagggagtcc
      181 ggtggatacc gggcgatcgt cgaggagtgc ggctttacgc actggctcat cgaacggctg
      241 aagatccacg agtgcgacct gaccgtcgtc gttcagcccc tcaaacgcgc ccgccacaag
      301 accgacaagc gggacgccgc gacactcgcc gagatcctct ggaccaaccg cgaccggctg
      361 gaggcgggaa gtcggctgca cggcatccgc caggtcatgg tgccgacgga gcaggaccgc
      421 caggctcggc ggctgaccaa cgcgaagatt cgcctggtgc gtcagcggac ggcggccatc
      481 aatctcatca agggcgtgct tcggcagttc aatctgatgc acgactgccc gacgaagacg
      541 ttccagacga aggcggccaa ggcctggctc cgctcgatcg agctgcccaa ggccgacaag
      601 atgatcctgg agctcgcgct ggagcagtgg gagctgctgg atcggcagat cgacacgctg
      661 gatcccgaga tcgatcgccg cgccgatgag agcagcgccg cgcaggtgat caagaccatt
      721 cccggagccg gcagtctgac cgcgctggtc ctggccagcc ggatcggctc gatcgatcgc
      781 ttcccccgac cctcgagcct ggccaacatg ttcgggctga cgccgacgat caacgacacc
      841 ggtgactcga ccgggcggct cgggggaatc accaagcacg gtcatcccaa cgtgcgattc
      901 ctgctcggcc agatggtcgc ccaggtgacc cgcgccgacc cgcatctgcg ggccattcat
      961 cggaagatcc gcaagcgacg cgggcgcaag acagcgatgg tggccacgat gcgccgcatc
     1021 acgtgccgcg tgtggcacat gctcaagacg ggtgaggcat acagaatcgg ccccggccgc
     1081 gccaccgcct cgacgccagg gcagtgatcg taggccgctg acgcggccac tgcggacagc
     1141 taccgcaggc gtcgaggctg ggtctgcgta tgtgcaaacg aaaacgcacc gcatggagaa
     1201 cacgaaaccg aacgagtcgt cgaacggcag ttggatcgac aggagcctcg gctcctgtcc
     1261 caccgaactg atcagctcga gaggctgcat ggcagcgtct ggaatggcaa gagccacagc
     1321 tcgccacgcg tcgtcctgat tggagcgatc ggctcggtgg acggtcgaga gaaatcatcc
     1381 gtgccaggcg tcacgcctgg gtgtcaccgc caagcgggac gcatctcacg aaggaatgtg
     1441 ttgggatccg gccactggcc ctcccgttga agaccgaccg accgccggag cgacagcgag
     1501 atggagaacc gacaacctcg gagccatcac cgatggggcg cggtcgaacc agccatgccg
     1561 acgaaaactc gttcagaaaa gacggtcgtt catgcttgac cgtacgagct ctatgaggaa
     1621 tgtcgcctcc actcaggagg aaacaccaac gcaccagcag gcgtccatgc ctgctgg
//
LOCUS       DBNHOX010000043         2269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_454032, whole genome shotgun sequence.
ACCESSION   DBNHOX010000043 DBNHOX010000000
VERSION     DBNHOX010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_454032"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="AC16UZ_00935"
     CDS             complement(<1..374)
                     /locus_tag="AC16UZ_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR6245270.1"
                     /translation="MAQKFNAETQQQGADESSQLHGILQSAMDAIITIDEQQKIVLFN
                     AAAERIFRCPAAQAVGGTLDRFIPERYRAGHRAHVRDFRAQGVTTRRMGDETVLHGLR
                     ADGEEFPLEASISRIETGGLGLL"
     gene            complement(546..>2269)
                     /locus_tag="AC16UZ_00940"
     CDS             complement(546..>2269)
                     /locus_tag="AC16UZ_00940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PHA/PHB synthase family protein"
                     /protein_id="HIR6245271.1"
                     /translation="LISGHGYNQDRMLHAAMARLTHGLSPASLGAAAGDWLTHLALSP
                     GKQQQLGEKALRKLHRLVLYQMQWARTRCPPCIEPLPQDTRFRHPDWQQYPFNLIYQG
                     FLLCQQWWWNATTEVRGVTPHHEQVMTFIARQLLDIVSPSNFLLTNPEVLRETLRTGG
                     MNLVKGAANWWDDAERLAAGRRPEGAEAYDVGKAVAVTPGKVILRNRLMELIQYRPTT
                     ARVYREPVLIVPSWIMKYYVLDLSPRNSLVRYLVRQGHTVFMISWKNPDALDRDLGMK
                     DYLRQGVMAALEAVSTVLPKRRVHAVGYCLGGTLLAIAAASLARDGDPCLRSVTLLAS
                     EVDFEEPGELSLFIDESQVAYLEDLMWDQGYLDGKQMAGAFTLLNSKDLVWSRIVRDY
                     LIGRRRPISDLMAWNADATRLPYRMHTEYLRDFYLKNDLAEGRYEVGGKPVSLGDIEV
                     PLFAVGTVSDHVSPWRSVYRIHLLTDTEVTFLLTTGGHNAGVVSEPGHANRSYQLDTR
                     KRNGKYVDPDTWRSTAPVSEGSWWPEWQRWLAAHSSGQGRRPPMGAPTHGYAPLEGAP
                     GQYVLAP"
BASE COUNT          410 a          744 c          720 g          395 t
ORIGIN      
        1 aggagcccga gccctccagt ctcgatgcgt gagatcgacg cctcgagcgg aaactcttca
       61 ccgtccgccc gcagtccgtg caggaccgtc tcgtcgccca tccgccgggt ggtcaccccc
      121 tgcgcgcgaa agtcccggac atgcgcgcgg tggcccgccc ggtagcgctc cggtatgaag
      181 cggtcgagcg tccctccgac tgcctgcgcc gccgggcaac ggaagatgcg ctcggcggcg
      241 gcgttaaaga gcacgatctt ttgctgctcg tcgatggtga tgatcgcatc catcgccgac
      301 tgcaggatac cgtgcagctg cgagctctcg tccgcccctt gttgctgagt ctccgcgttg
      361 aacttctgcg ccatcagaaa acgttccttg gatcgctgcg cgcgcgcagc accgtcacct
      421 ctgccacgaa tgcggggttc gacacgggaa tggccgcccg atcgcggcgt aattgcgatt
      481 gcggaagtat accgaaagcg ggcatcggag gcgctcgcgc ccccgatgcc cggcgcgcac
      541 acgcgctaag gcgccagaac gtactgcccc ggagccccct caagtggagc gtagccgtgt
      601 gttggcgcgc ccattggcgg ccgacgcccc tgcccgctgg aatgcgcggc aagccagcgc
      661 tgccactccg gccaccacga gccctcgctc accggcgctg tcgaacgcca tgtgtcgggg
      721 tcaacgtact tgccgttccg cttacgggtg tcgagctggt agctgcgatt ggcatgcccg
      781 ggctcgctca cgacgccggc gttgtgccct ccggtggtca gaaggaaagt gacctccgta
      841 tcggtgagga gatgaatccg gtatacggat cgccagggag agacgtggtc gctcaccgta
      901 ccgacggcga ataatggcac ttcgatatcg cccagagaaa cgggtttgcc gccgacctcg
      961 tagcggccct cggcgagatc gttcttgaga tagaagtccc gcagatattc cgtgtgcatg
     1021 cgatagggca ggcgcgtggc atcggcgttc cacgccatca agtcgctgat ggggcggcgg
     1081 cgcccgatga ggtaatcgcg cacgatgcgc gaccacacga gatccttcga attcagcagc
     1141 gtgaaggcgc cggccatctg ctttccgtcc agatagccct ggtcccacat cagatcttca
     1201 aggtaggcga cctggctctc gtcgatgaag agcgaaagct cccccggttc ctcgaaatcg
     1261 acttccgacg ccagcagcgt gaccgaacgc aggcacgggt cgccgtcccg tgccaggctc
     1321 gctgcggcga tcgcgagcaa tgtgcccccg aggcaatatc ccaccgcgtg aacacgtcgc
     1381 ttgggcagca ccgtcgatac ggcctcaagc gcggccatga cgccctgcct cagatagtcc
     1441 ttcatgccga ggtcgcggtc cagggcatcg gggttcttcc acgaaatcat gaagacggtg
     1501 tgcccctgac gcacgagata gcgcacaagc gagttgcggg gtgagagatc gagaacgtaa
     1561 tacttcatga tccaggaagg gacgatcagc accggttcac ggtagacacg cgccgtcgtg
     1621 ggccggtact ggatgagctc catcagccgg ttccgcaaga tgacctttcc cggcgtcact
     1681 gcaacagcct tgccaacgtc ataggcttcg gcgccttccg gccggcgccc ggccgcgaga
     1741 cgctccgcgt cgtcccacca gttggcagcg cccttgacga ggttcatacc gccggtacgg
     1801 agcgtctccc gcagcacctc gggattggtc agcaggaaat tcgagggcga cacgatgtcc
     1861 aggagttgac gggcaatgaa cgtcatgacc tgttcgtggt gcggcgtcac gccgcgcacc
     1921 tccgtagtcg cgttccacca ccactgctga caaagcagaa agccctggta gatgaggttg
     1981 aacggatact gctgccaatc gggatgacga aagcgggtgt cctgcggcaa cggctcgata
     2041 cagggcgggc aacgcgtgcg cgcccattgc atctggtaga gaaccagccg gtgcagcttt
     2101 cgcagcgcct tttccccgag ctgctgctgc ttgccgggtg agagggcaag atgcgtgagc
     2161 cagtcgccgg ccgctgctcc cagcgacgca ggcgacaagc cgtgcgtcaa ccgcgccatc
     2221 gccgcgtgca gcatgcggtc ctggttgtag ccgtgcccgc tgatcagct
//
LOCUS       DBNHOX010000044         3529 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_968257, whole genome shotgun sequence.
ACCESSION   DBNHOX010000044 DBNHOX010000000
VERSION     DBNHOX010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_968257"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="AC16UZ_00945"
     CDS             complement(<1..443)
                     /locus_tag="AC16UZ_00945"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6245272.1"
                     /translation="MGTFHGKHKGPGALATGATACLLAAAATAKEFNITAEVVEGSFE
                     PASYSPYAGRNFPSRLLWGDTHLHTSLSLDARAAGVILDPEAAYRFARGEEIRTTAGM
                     KIRIGQPLDFLVITDHSDAMGAMNEVVAGNPDLLRDPTVRDWHQR"
     gene            658..930
                     /locus_tag="AC16UZ_00950"
     CDS             658..930
                     /locus_tag="AC16UZ_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245273.1"
                     /translation="MIRKMFSAALLVVGLAGLSSSAWAAQNWAEFCAEKAAAGDDAAI
                     CNVHCNPEPPSSCSNPEIDGSQAGIALALILGIGLLVSERRRKPTA"
     gene            complement(1034..2707)
                     /gene="xrtE"
                     /locus_tag="AC16UZ_00955"
     CDS             complement(1034..2707)
                     /gene="xrtE"
                     /locus_tag="AC16UZ_00955"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase E/protease, VPEID-CTERM system"
                     /protein_id="HIR6245274.1"
                     /translation="MRLAINQAFSYLLTMQSGIPLSGKAPNLFGLQLALVSALLVSEM
                     FFISYRYDAWTLLQTTDSPWLTWLAYGGYFLKSAIAFVAALLVTIGTRLKAHYDDLVT
                     VTAPRSPWALLLAQLAAYAVLVWLTHLIFADPAEGAPPGAIIVGAWVLATTATVCIWL
                     SLIAPFSYWRRLVRQERTPIAASVLVASVALVIGVSSQQLWGPLSQMTFASVEWVLWL
                     IDDEVYVDRERMILGLPYFQSRIAEPCSGYEGIGLVTVFTTLYLAIYRDHFKFPNALL
                     LFPIGIIAIWCANVLRIVALIVIGNGFSPAVAVGGFHSFAGWIAFITVAVAMLVYADR
                     HHFFATKTAPRSGARLGMPGVLLVPFTFLLATTLLTLAFSGDFVWLYPARVIVTGIVL
                     AMLWRRLELGSWRPGLIPVAAGFAVFVLWIAMVPVDEAGSAAFTAALTEAGPLAAAGW
                     MIFRVIGSVITVPIAEELAFRGYLMIRLSGSKVGETDRLRFSWIALIVSSVLFGLMHA
                     DWLAGIMAGLAYGLVRYHRGSLTDAIVAHAMTNLLLTVYVITTGHWSVW"
     gene            2723..3505
                     /locus_tag="AC16UZ_00960"
     CDS             2723..3505
                     /locus_tag="AC16UZ_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HIR6245275.1"
                     /translation="MRHRLLGLADHRSPFSGCGRAELLRAALSWGRRVRNRRESVLND
                     RLTIDYFSDVLCVWAWIAQRRIDEMNDQWGETVRLGFHCVDVFGDAGAKVAAGWSARG
                     GYEGFAQHVREVAIRYEAPAVHEYLWTKVRPVTSGTAHLVLKATEIAESPLEMTRLSR
                     ALREAFFVDARDVGDLGVCLEVAQGLGLAAAELKRHVDSGAAMAALLADYRKAEERGI
                     RGSPSWVMNEGRQVLYGNVGYRILNANIEELLRHPEQEASWC"
BASE COUNT          694 a         1049 c         1169 g          617 t
ORIGIN      
        1 atgcgctgat gccagtcccg cacggtgggg tccctcagca gatccggatt gcccgccacc
       61 acctcgttca tggctcccat ggcatccgag tgatcggtga tcaccaggaa gtccagcggt
      121 tggccgatgc ggatcttcat cccagccgtt gtgcggatct cctcgccccg ggcgaaccgg
      181 taggcggcct ccgggtcgag aatcacgccg gcggcccggg cgtcgagcga caggctcgtg
      241 tgcaggtggg tatcccccca aagcagccgg ctggggaagt tgcgccccgc gtacggcgag
      301 tagctggcgg gctcgaagga cccctccact acctcggcgg tgatgttgaa ctccttagcc
      361 gttgccgccg cagcgagcag gcaggccgtc gcccccgttg cgagcgcgcc agggcctttg
      421 tgttttccgt gaaatgtgcc catccgtgcc cccgcggcgt tgtttcctgg taacacggcc
      481 atactcgggc atcaggacca ccgggtaaac tccgaccccg gctctgacct cactgcggcg
      541 gctactcata aaattcttac aattgtgact ttgcagggtt tcagtggctg tgataaaaag
      601 tgatcagagc gtaggtttcc tgacgttctt gggtgttgta tttgggggtt attgatcatg
      661 attcgtaaaa tgttttccgc ggccttgttg gtggtcgggt tggctggcct gtcgagctcc
      721 gcttgggcgg cacagaactg ggcagagttt tgtgcggaga aggctgctgc cggtgacgac
      781 gctgcgattt gcaacgttca ctgcaatcct gaaccgccgt catcgtgcag caatccggag
      841 attgatggca gccaggcggg tatcgccttg gccctgattc tcgggatcgg attgctcgtc
      901 tcggagcgcc gccgcaagcc gactgcctga aggcattccg caacttttca gcgagcctct
      961 cgccaacgga tgggggagag gtgctcgcac acctaaaatt cgatgattgg tgcggaaagc
     1021 gctaggaatg gtctcaccag accgaccagt gtcccgtcgt gatgacgtag accgtcaaga
     1081 gtaggttcgt cattgcgtgc gcgacgatcg cgtccgtcag agaaccgcgg tgatagcgca
     1141 ccagaccgta ggccagcccc gccataatcc cggccagcca gtcggcgtgc atcagcccga
     1201 atagaaccga cgagacgatc agagcgatcc acgagaatcg caagcgatcg gtctccccaa
     1261 ccttgctccc cgatagccga atcatcagat agccccggaa ggcgagctcc tcggcgatgg
     1321 gcacggtgat taccgaaccg atgacccgga agatcatcca gcctgcggca gccagcgggc
     1381 ccgcttcggt gagcgcggcg gtgaacgctg cgctgcccgc ttcgtccacg ggcaccatgg
     1441 cgatccacag cacgaacacg gcgaaacctg cggcgaccgg aatcagcccg ggtcgccagg
     1501 aaccgagttc cagtcggcgc cagagcatcg ccagcacgat gcccgtgacg atgacccgcg
     1561 cggggtagag ccagacgaaa tccccagaga acgccagtgt cagcagcgtc gtcgccagca
     1621 gaaaggtaaa aggaaccagc agaacgcctg gcatgcccag gcgggcgccg gatcgaggtg
     1681 ccgttttcgt cgcgaagaaa tgatgcctgt cagcgtagac cagcatggcg acggccaccg
     1741 tgatgaaggc gatccagccg gcgaagctgt gaaatccgcc gacggcgacg gcgggggaga
     1801 agccgttgcc gatgacgatc aacgcgacga tccgcagaac gttggcgcac cagatggcga
     1861 tgatcccgat gggaaacagc aacagggcgt tgggaaactt gaagtggtcc cggtagatgg
     1921 cgagatagag cgtggtgaaa acggtgacca ggccgatgcc ttcgtatcct gagcagggct
     1981 ccgcaattcg gctctgaaag tagggcaggc ccagtatcat ccgctctcga tcgacgtaaa
     2041 cctcatcgtc gatcagccag agcacccatt ctacagaagc aaacgtcatc tggctcaacg
     2101 gcccccacag ctgctgagat gacacgccga tgaccagcgc caccgaggcg acgagcacgg
     2161 aagccgcgat tggtgtgcgt tcctggcgga ccaggcgccg ccagtaggag aacggggcga
     2221 tcagtgagag ccagatgcaa accgtcgcag tggtggccag aacccatgcg ccgacgatga
     2281 tcgcgccggg cggggcgccc tccgccggat ctgcaaagat caggtgcgtc agccagacca
     2341 ggacggcgta cgcggcaagc tgggcgagga gaagcgccca tggcgagcgg ggcgccgtca
     2401 cggtcaccag atcgtcgtag tgggctttga gccgcgtgcc aatcgtgacg agcagcgcgg
     2461 ccacgaaggc aatcgcgctc ttaagaaagt agccgccgta agcgagccag gtgagccagg
     2521 gactgtcggt agtttgcagc agcgtccagg cgtcgtagcg gtagctgatg aagaacatct
     2581 cgctcacgag cagggcgctc actagagcga gctgcagccc gaatagattt ggcgctttgc
     2641 ctgaaagggg aatgccgctc tgcatggtca gaagatagga gaaggcctgg ttgatcgcta
     2701 gtctcactta caaactggta acgtgcgaca caggttgctg ggcttggctg accacagatc
     2761 tcctttctcg ggttgcggtc gcgcggagct cttgcgtgct gcgttatcat ggggccgacg
     2821 tgtacggaac cggcgagaat ctgtcctgaa cgaccgcctg accatcgact acttctccga
     2881 cgtgctgtgc gtgtgggcct ggatcgcgca gcggcgcatc gacgagatga acgaccaatg
     2941 gggcgagacg gttcggctgg gcttccactg cgtcgacgtc ttcggcgacg caggcgcgaa
     3001 ggtggcagcg ggttggagcg cccgtggcgg ctacgaagga tttgcgcagc acgtgcgcga
     3061 ggtcgccatc cgctacgagg cgcctgccgt ccacgagtat ctctggacga aggtcaggcc
     3121 ggtgacgtcg ggcaccgccc atctggtgct caaggcaacg gagattgcgg agagcccact
     3181 ggagatgact cgcctgtctc gggccctgcg cgaggcgttc ttcgtcgatg ctcgagacgt
     3241 cggcgatctc ggcgtctgcc tggaggttgc tcaggggctt ggtctcgccg ccgcggaact
     3301 gaagcgacac gtggattctg gcgcggccat ggccgccctt ctggcggact atcgcaaggc
     3361 ggaggagcgg ggcatcaggg gaagcccgtc ctgggtgatg aacgagggtc gccaggtgtt
     3421 gtacggcaac gtcggctacc gcattctgaa tgcaaatatc gaggagctgc tgcgccaccc
     3481 ggaacaggaa gccagctggt gctaggttgc cgcgcgtccg gcgcccggc
//
LOCUS       DBNHOX010000045         1856 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1096980, whole genome shotgun sequence.
ACCESSION   DBNHOX010000045 DBNHOX010000000
VERSION     DBNHOX010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1096980"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="AC16UZ_00965"
     CDS             <1..528
                     /locus_tag="AC16UZ_00965"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6245276.1"
                     /translation="AAFREGDRIRVGDVVGTVEEIMLMVTRVRTPKNECVIMPNSNIL
                     NTDVVNYSQMAKHEGLILYTEVGIGYDTPWRQVEAMLKLAAERTEGLLKDPPAFVLQK
                     SLGDFAVIYELNVHTRDETQMPRLYSALHANIQDVFNENAVQIMSPAYEADPEAPKIV
                     PPEKWFETPASKPGS"
     gene            543..1799
                     /locus_tag="AC16UZ_00970"
     CDS             543..1799
                     /locus_tag="AC16UZ_00970"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR6245277.1"
                     /translation="MLIACLKCTGCGAEYPPDHLMNLCPVDGRPVQIILDLERLLSEQ
                     PELAWYHPERRDMWRFGGLLPLDAGNAEDRPRIVTLGEGCTPCLDYGDHALARKAGFR
                     LRMKDEGIAHPGFGANPTGSFKDRGMAMAVSMARWHGLDRLAVPTQGNAGDSLCAYAE
                     AAGMRVVVAMPQDTPMPILGRVAALAHGSDRFELLLVEGTIREAGELLKRDWIPGGWF
                     SVATFQEPGWRIEGKKTLGLELAEPTQPGEPWSLPDVIVYPTGGGTGILGMWKAFSEL
                     ECLGLIGPERPRMVCVQAEATAPLVHAFEHGLEDTAAAQAGATLAYGLNVPGGVGHFR
                     VLQIVRESGGAAVAIAEAEMQACFTADWQARHGWVCPEGAACLAALPRLLDEGLIQRG
                     DDVVVFNTGSLEKYLPELRHLLGEPG"
BASE COUNT          316 a          600 c          648 g          292 t
ORIGIN      
        1 gccgccttcc gcgagggtga ccggattcgt gtcggcgacg tggtcggcac ggtggaggaa
       61 atcatgctga tggtcacccg ggtacgaacg cccaagaacg aatgtgtcat catgccgaat
      121 tccaacatcc tcaataccga cgtggtcaat tactcgcaga tggcaaaaca cgaaggcctg
      181 attctgtaca ccgaggtcgg catcggatac gacacgccct ggcgccaggt ggaggccatg
      241 ctgaaactgg cggccgaacg caccgagggg ctgctgaagg acccgcccgc cttcgttctg
      301 cagaaatccc tgggcgactt tgcggtcatt tacgagctga acgtccacac ccgggacgag
      361 acgcagatgc cgcggctcta ctcggcgctg catgccaaca tccaggacgt gttcaacgag
      421 aatgccgtgc agatcatgtc gccggcctac gaggcggacc ccgaggcgcc caagatcgtg
      481 cccccggaaa aatggttcga gacgcccgcc agcaagccgg gttcctgaaa cgtgagccga
      541 ccatgctgat tgcctgcctc aaatgcaccg gctgcggggc cgagtacccg cccgaccatc
      601 tgatgaacct ctgccccgtg gacggccgcc cggtgcagat catcctggac ctggaacgcc
      661 tgctgtccga gcagccggag ctcgcctggt accatcccga acgccgggac atgtggcggt
      721 tcggcggcct gctgccgctg gacgcgggca acgcggagga caggccccgc atcgtcaccc
      781 tcggcgaggg ctgcacgcca tgcctggact atggtgacca tgcgctggcc cgcaaggccg
      841 ggttccggct gcgcatgaag gacgagggaa tcgcccaccc gggcttcggg gccaacccca
      901 ccggcagctt caaggaccgg ggcatggcga tggcggtctc gatggcgcgc tggcacgggc
      961 tcgaccgcct ggccgtgccc acccagggca atgcgggcga ctcgctgtgc gcctatgccg
     1021 aggcggcggg catgcgggtg gtggtcgcca tgccgcaaga cacgcccatg ccgatacttg
     1081 gccgggtcgc cgcactggcc catggatccg accgcttcga gttgctgctg gtcgagggca
     1141 cgatccgcga ggccggcgag ttgctcaagc gggattggat tccggggggg tggttcagcg
     1201 tcgccacctt ccaggagccg ggctggcgca ttgaaggcaa gaagacactg ggactggagt
     1261 tggcggagcc gacgcaaccc ggcgagccct ggtccctccc cgacgtcatc gtgtatccga
     1321 ccggtggcgg caccggcata ctcggaatgt ggaaagcgtt ctcggaactg gagtgcctgg
     1381 gcctgatcgg gccggaacgg ccgcgaatgg tgtgtgtgca ggccgaggcg accgccccgc
     1441 tggtgcatgc gttcgagcac ggcctcgaag acacggccgc ggctcaggcc ggtgcaacgc
     1501 tggcctacgg gctgaacgtg cctggcggcg tgggccattt ccgggtgctg cagatcgtgc
     1561 gggaaagcgg tggcgccgcc gtcgccattg cggaagcgga gatgcaggcg tgctttaccg
     1621 ccgactggca ggcacggcac ggctgggttt gcccggaagg ggcggcctgc ctggcggccc
     1681 tcccgcgcct gctcgatgag ggcctgattc agcgcgggga cgacgtcgtg gtgttcaaca
     1741 cgggatcgct tgagaagtac cttcccgagc tgcggcacct gcttggcgag ccgggctgag
     1801 gccgtcagcg cgaatcacgc cggggatcag gtgcttcccg gcgtcccccc ggactc
//
LOCUS       DBNHOX010000046         7397 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_775750, whole genome shotgun sequence.
ACCESSION   DBNHOX010000046 DBNHOX010000000
VERSION     DBNHOX010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_775750"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="AC16UZ_00975"
     CDS             complement(<1..936)
                     /locus_tag="AC16UZ_00975"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6245278.1"
                     /translation="MSERPTTITVSRVWDGEALCDADAVRIENGRIAAVGDARELSKG
                     AVAVHCPGATALPGLIDAHVHLELNPDNHKPPESTSPDEVPRMLERAAAMVRAGITTA
                     RDLGGGAWLELGIRDKTAAGQAIGPRLLCSGQPITCPGGHCHFWGGEAGSAEEALSVL
                     ERQVTQGVDLIKVMATGGRMTNGSDPLAAQFDLETLSEITAAARAHGLPVAAHCHGTT
                     GIELAARAGVSTIEHCSWAGAEGWASDFQEPVANVILERGVWVSPTVNRGWQRMLDSR
                     EGRVLGRVRAAYRAMLELGIPFIASTDAGIPGVFHH"
     gene            complement(933..2312)
                     /locus_tag="AC16UZ_00980"
     CDS             complement(933..2312)
                     /locus_tag="AC16UZ_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6245279.1"
                     /translation="MVELIPIFMFLAVFAALLAGYPVALTLAGVALLFALGGMAGGTF
                     DGRDLGFLPGRLFGIITNPTLIAVPLFVFMGVMMEKTRIAENLLSSLSRLMAGTPGGL
                     AFAVIVVGMLMAASTGIVGASVVTLGLMSLPTMLRDRYDPAFASGTISATGTLGQIIP
                     PSIALVLLGEVLSNAYQQAQLNQGIFAPDTVSVGDLFAGAVVPGLILVGLYLAYTMLV
                     AFLRPGTAPAASSVADAVPDGSGWQLLGGLLPPMLLIVAVLGSILAGYATPTEAAGVG
                     AAGATILAAAYRALDVGVLRQVCRSTFDTTAMVFFILVGASLFSLVFRGYGGDDLVHS
                     WFNDMPGGTWGALAIVMLVIFLLGFMLDFIEITFVVVPIVGPVLLAMGVDPIWLGVLI
                     AVNLQTSFLTPPFGFALFYLRGVAPPTVSTADIYRGVAPFVLLQILLLCLLFIWPSLA
                     TWLPERIYR"
     gene            complement(2317..2778)
                     /locus_tag="AC16UZ_00985"
     CDS             complement(2317..2778)
                     /locus_tag="AC16UZ_00985"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR6245280.1"
                     /translation="MAWLTALMVVLMVTVVVLRYAFDQGAIPIQEAIVYLHATVIMLG
                     IPCALRDGVHVRVDILHSRLSERARGIIDVAGHLLFLVPTCLLILYFSIPYARNAWLV
                     LEGSPEVGGIPAVFLLKTLIPVMAVALLLQGLAETVRRLPIILPSHFGSER"
     gene            complement(2832..3740)
                     /gene="ubiA"
                     /locus_tag="AC16UZ_00990"
     CDS             complement(2832..3740)
                     /gene="ubiA"
                     /locus_tag="AC16UZ_00990"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HIR6245281.1"
                     /translation="MEKTRQPPLSAVIAYARLMRLDRPVGTLLLLWPTLAALWVAAEG
                     LPPVDLIVVFAVGTFLMRAAGCVVNDYADRNLDPGVARTANRPLATGAVSTNAALGAF
                     FVLAFLALTLLLFLNTLTRWFAVAGLAIAVAYPFMKRWTYLPQVVLGAAFSWGIVMAF
                     TAVRHVVPEPGWLLFLASVLWIVAYDTMYAMVDREDDLRVGIKSTAILFGEADRLMIG
                     LLQTGTILTLVLVGQRLGYHSAYFWGIAVAAGLFCYQQYLIRDRSREGCFRAFANNVW
                     VGFSLFVGVAWETMTRGAFQPGGGLS"
     gene            3900..4067
                     /locus_tag="AC16UZ_00995"
     CDS             3900..4067
                     /locus_tag="AC16UZ_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0043448 - alkane catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HIR6245282.1"
                     /translation="MRKWQCIVCGWIYDEAEGYEEEGIAPGTAWEDVPEDFVCPECGV
                     SKEDFEMIEVG"
     gene            4084..5352
                     /locus_tag="AC16UZ_01000"
     CDS             4084..5352
                     /locus_tag="AC16UZ_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012593.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR6245283.1"
                     /translation="MNLTIRILLGMGLGLVLGSSIQAAEFDPSHWVHVYLVGGLIDAG
                     GQIFVRSLQLMVVPLVFVSLVCGAASLSATDSIGRVAGKTVALYLFTTAVAISIALLL
                     ATIVQPGAGVAPASLGTDFQPAEPTSIKDTLVSIIPTNPVSAMAEGRMLQIILFALLL
                     GLALSRSGQAGRRLAGVFDDLNEALMKLITLLIKLAPIGVFCLMAKLFATLGWSEITR
                     LLQYFLTVVGALLIHVTLVYPTLLMVLGRVSPIRFFGKMREPLLVAFSTASSGATLPV
                     TLRTVEERVGVKNEVASFVVPLGATINMDGTAIMQGVATVFIAQLYGIELGMSQFLII
                     ILTATMASVGTAGVPGVGMIMLVMVLEQVGLPLDGIVFILAVDRLLDMLRTAVNVAGD
                     GMVATVVARSENELDLDTFEDPDAGRVIGS"
     gene            complement(5374..7386)
                     /gene="recG"
                     /locus_tag="AC16UZ_01005"
     CDS             complement(5374..7386)
                     /gene="recG"
                     /locus_tag="AC16UZ_01005"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019694287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR6245284.1"
                     /translation="MIAEQRVESLKGVGPSLKAKLERLGIFRVGDLLLHLPIRYQDRS
                     RVVPLRDVHPQQECLVQGQVVDSSLGYGRRRSWLVTIEDGTGYLGLRFFHFSKRQQSG
                     LQPGTYVRCFGEIRFGPRGGGPGLEMAHPEYRAFTSEPPGPDPELTPVYPTTKGLGQN
                     RLRGLIDQALSLEWPSDSGAPYQTLAYLHRPPADAPAADIERAQERVALDELTAYYLV
                     MKQRQRDRAKSRALPLPQSQQLGRELLRRLGFRLTHAQRRVITEVLEDLSSETPMLRL
                     LQGDVGSGKTVVAAFAAIRAAEHGRQTALMAPTEILAEQHYVNFSAWLEPLGIPVVLL
                     TGSQSAKERAARQQALEEGEALVAVGTHALFQDKVNFNALALTIVDEQHRFGVHQRMA
                     LRSKGAAPHQLVMTATPIPRTLTMALYADMDVSVLDELPPGRTPIDTRVISDARKEEV
                     IRSAADAMARGEQVYWVCPLIEDSEQLDAAAAEPTAELLAERLEPPIGLLHGRMTGEQ
                     KAKAMMDFKEGRTRLLVATTVIEVGVDVPNATLMVIENPERMGLAQLHQLRGRVGRGE
                     KASRCLLVYKTPLSAAARQRLSVMRESQDGFHIAEKDLELRGPGELLGARQTGEQQFR
                     VADLARHSHLIAEVLARGERLLQTDPTTARELLNAWAPEDNGHIAV"
BASE COUNT         1308 a         2364 c         2522 g         1203 t
ORIGIN      
        1 gtgatgaaac accccgggaa ttccggcgtc cgtagaagcg atgaagggaa ttcccagctc
       61 cagcatcgcc cggtaggcgg cacgaacgcg gccgagcacc cgcccttcgc ggctgtccag
      121 catgcgctgc cagccacgat tgacggtggg cgacacccag acgccgcgct cgaggatcac
      181 gtttgccacc ggctcctgga agtccgatgc ccaaccttcc gcaccggccc aggagcagtg
      241 ctcgatggtg gacacgccgg cgcgggcggc caactcgatg cccgtggtgc cgtggcagtg
      301 ggcggcgacg ggcagcccgt gcgccctggc ggccgccgtt atctcgctca gcgtctccag
      361 gtcgaactgg gcggccagcg gatcggatcc gttggtcatc cggccgcccg tggccatgac
      421 tttgatcagg tccacgccct gggtcacctg acgctccagc acggacaggg cttcttcggc
      481 ggagcccgct tccccgcccc agaagtggca gtgaccgccg ggacacgtga tgggttgccc
      541 ggagcagagc agtcggggcc cgatagcctg gccggccgcc gttttgtccc ggatgccgag
      601 ctcgagccag gcaccgccgc cgaggtcgcg cgcggtggta attcctgccc ggaccatggc
      661 cgcggcccgt tccaacattc tcggcacctc gtccgggctc gtcgactcgg gaggcttgtg
      721 attgtcgggg ttgagctcca ggtggacgtg ggcgtcgatg agcccaggga gcgcggtggc
      781 cccggggcag tggacggcca cggcgccttt gctcagctcc cgggcgtcgc cgaccgccgc
      841 gatgcgtcca ttttcgatgc gaacggcatc ggcgtcgcaa agagcctccc cgtcccatac
      901 gcggctcacc gtaatggtgg ttggccgctc gctcaccggt agatgcgctc cggtaaccag
      961 gtcgcaaggc tgggccagat gaagagcagg cacaggagca ggatctgcag caacacgaac
     1021 ggggccacgc cgcggtagat gtcggctgtg gaaaccgtgg ggggagccac gccgcgcaga
     1081 tagaagagcg cgaagccgaa cggtggcgtc aggaacgagg tctggagatt gaccgcgatc
     1141 agcaccccca gccagatcgg gtctacaccc atggccagaa gcacggggcc gacgatgggc
     1201 accacgacga aggtgatttc gatgaagtcg agcatgaagc cgagcaggaa gatcaccagc
     1261 atcacgatgg cgagcgctcc ccaggtgccg cccggcatat cgttgaacca gctgtgaact
     1321 aggtcgtcgc cgccgtatcc cctgaagacc agagaaaaca aggaggcgcc gaccaggatg
     1381 aagaaaacca tggccgtggt gtcaaacgtc gaccggcaca cctggcgcaa gacgccgacg
     1441 tccagcgcgc gataagccgc cgccaggatg gtcgctccgg ctgcgccgac accggcggcc
     1501 tccgtcgggg ttgcgtagcc cgcgaggatg gaccccagca cggcaacgat cagcagcatg
     1561 ggaggaagca gcccgcccaa gagctgccac cctgagccgt ccgggaccgc gtccgcgacc
     1621 gagctggcgg cgggcgcggt gcccggccgc aggaaggcga ccagcatggt gtaggccaga
     1681 tagagcccga ccaggatgag tccgggcacc accgcgcctg caaacaggtc cccgaccgac
     1741 accgtgtccg gggcgaagat gccctgattc aactgcgctt gctgataggc gttcgagaga
     1801 acctcgccca gcagcaccag ggcaatggac ggaggaatga tctggcccag cgttcccgtc
     1861 gcgctgatgg tgccgctagc gaaggccggg tcgtagcgat cgcgaagcat ggtgggtaga
     1921 gacatcagcc cgagggtgac caccgaggcg ccgacgatac cggtgctcgc agccatgagc
     1981 atgccgacca cgatgacggc aaaagccagc cccccgggcg ttcccgccat cagccgcgaa
     2041 aggctgctga gcaggttctc ggcgatccgc gtcttctcca tcatcacgcc catgaagacg
     2101 aacagcggca ccgcgatgag cgtggggttc gtgatgatgc cgaacagccg gccgggcagg
     2161 aaacccagat cacggccgtc gaaggttccg ccggccatcc cgcccagggc gaacagcagg
     2221 gcaaccccgg ccagggtcag cgctacgggg tatccggcca gcagggcggc gaagacggcc
     2281 aggaacatga agatcggtat gagctcgacc acgttgtcag cgctcgctac cgaaatggga
     2341 gggcagaatg atgggcagcc ggcgcacggt ctccgcgagg ccctgcagca gcagggcaac
     2401 cgccatgacc ggaatgagcg ttttcaggag aaagacggcc ggaatgccgc cgacctcggg
     2461 tgatccctcg agcacgagcc acgcgttgcg ggcataggga atgctgaaat agaggattag
     2521 caggcaggta ggcacgagga acagcaggtg cccggcaacg tcgatgatcc cccgggcgcg
     2581 ttcgctcaag cgcgagtgaa ggatgtccac cctgacgtgg acgccgtctc tcaaggcgca
     2641 gggaatgccg agcatgatca ccgtggcgtg cagatagacg atggcttcct gaatcggtat
     2701 cgcgccctgg tcgaacgcgt agcgcagaac gacgacggta accatcagca cgaccatgag
     2761 ggcggttagc caggccacgc tgcgggccgt cgcctcggtg acgcgatcgg cgtgctcgag
     2821 cagccgtgtg ttcagctcag ccccccacct ggctggaagg cgccgcgggt catggtttcc
     2881 catgcgacgc cgacgaacag cgaaaaaccg acccacacgt tgttggcgaa ggcgcggaag
     2941 caaccctccc ggcttcggtc ccggatcagg tattgctgat agcagaacaa gccggccgcg
     3001 acggcgatgc cccagaagta ggcgctgtgg tagccgagcc gctggccgac caggaccagg
     3061 gtgaggatcg tgccggtctg cagaagcccg atcatcagac ggtcggcctc gccaaacagt
     3121 atggccgtgg acttgatccc cactcgcaga tcgtcctccc ggtccaccat ggcgtacatg
     3181 gtgtcgtagg cgacgatcca gagaacgctt gccaggaaaa gcagccagcc gggctccggc
     3241 accacgtgcc gtaccgcggt aaacgccatg acgatgcccc agctgaacgc ggcgcccagc
     3301 accacctgcg gaaggtaggt ccatcgcttc atgaagggat aggccaccgc gatggccagg
     3361 cctgcgacgg cgaaccatcg ggtcagcgtg ttgaggaaga gcagcagggt cagggcgagg
     3421 aaggccagca cgaagaaggc gcccagcgcg gcgttggtgc tgacggctcc ggtcgcaagg
     3481 ggtcgattag cggttctggc gacgccggga tcgaggttac gatcggcgta gtcattcacc
     3541 acgcagccgg cggcccgcat cagaaacgtg ccgacggcaa agaccacgat gagatccacg
     3601 ggcggcagcc cctcggcggc tacccagaga gcggccaggg tcggccacag gagcagcagg
     3661 gtgcccacgg gccggtcgag gcgcatcagg cgcgcgtacg cgatcacggc ggagagcggc
     3721 ggctgacggg ttttctccat gttcgcgcgg gcgggcttga gcttggcggc ggagtgtagc
     3781 gtttcgccac caggcaggcc aggcggccgc cggccgcacg attgattttt ccgcggggga
     3841 tgagtagttt atcgcgcctt tgcgccggcg ccggcttgaa caaagggaag cgaccaagaa
     3901 tgcgaaaatg gcaatgcatc gtctgcggct ggatctacga cgaagccgag ggttatgagg
     3961 aagagggcat tgcgccgggt acggcttggg aggatgtgcc cgaggatttc gtttgcccgg
     4021 agtgcggcgt cagcaaagaa gacttcgaga tgatcgaggt cggctgaggc cgattagaac
     4081 gtcgtgaacc ttacgatccg cattctactc ggcatgggct tggggctcgt cctcggctct
     4141 tcgatccagg cggcggaatt cgatccttcc cattgggtcc acgtctacct cgtcggcggc
     4201 ctgatagacg ctggcggcca gatattcgtt cgcagcctcc agctcatggt ggttcccctg
     4261 gtattcgtgt cgttggtgtg cggtgctgcc agcctgtcgg cgacggacag catcggtcgg
     4321 gtggccggaa agaccgttgc cctttatctg ttcaccacgg ccgtggccat ttccatcgcc
     4381 ctgctgcttg caacgatcgt tcagcctggt gccggcgtcg cgccggcctc cctgggaacc
     4441 gacttccagc cggcggagcc gacgagcatc aaggacacgc tggtctccat catcccgacg
     4501 aacccggtta gcgccatggc ggaaggcagg atgctgcaga tcatcctgtt cgctcttctg
     4561 ctcggcctgg ccctgagccg gtcggggcag gccggccggc ggctggccgg cgttttcgac
     4621 gatctgaacg aagcgttgat gaagctgatc acgctgctga tcaagctcgc gcccatcggg
     4681 gtcttctgcc tgatggcgaa gctgttcgcc accctgggct ggtcggagat cacgcggctc
     4741 ctgcagtatt tcctgacggt ggtgggcgct ttgctcattc acgtcaccct ggtctacccg
     4801 acgctgctaa tggtgctggg cagggtcagc ccgatccgtt tcttcggcaa gatgcgcgag
     4861 ccgctgctgg tggccttcag caccgcctcg agcggggcga ccctgccggt caccctgcgc
     4921 accgtggagg agcgggttgg cgtgaaaaac gaagtggcaa gcttcgtcgt gcccctgggc
     4981 gcaaccatca acatggatgg aaccgccatc atgcaggggg tggcgacggt gttcatcgcg
     5041 cagctgtacg gcatcgagct gggaatgtcg cagttcctca tcatcatcct cacggcgacc
     5101 atggcctccg tcggcacggc gggggtcccc ggcgtcggca tgatcatgct ggtcatggtg
     5161 ctggagcagg tcgggctgcc tctcgacggc atcgtcttca tccttgccgt ggaccggctg
     5221 ctggacatgt tgaggacggc ggtcaatgtg gccggtgacg gcatggtggc gaccgtggtg
     5281 gcccggtccg aaaacgagct ggatctcgac acgttcgagg accccgatgc cggccgtgtg
     5341 atcggctcat aagagctgcc cggaagggcc ctcctacacg gcgatgtgcc cgttgtcctc
     5401 gggtgcccag gcattgagaa gctcccgggc cgttgtcggg tccgtctgca ggaggcgttc
     5461 gccgcgtgcc agcacctcgg caatcagatg gctgtggcgg gcgagatcgg caactcggaa
     5521 ctgctgctcg cccgtctggc gcgcgcccag cagctcgccc ggtccgcgca gctccaggtc
     5581 cttctcggcg atgtgaaagc cgtcctggct ctcccgcatg accgaaagac gctggcgtgc
     5641 cgccgcgctc agcggtgtct tgtagaccag caggcatcgc gatgctttct ctccccgccc
     5701 gaccctgccg cgcagctgat gcagctgtgc caagcccatg cgctccgggt tttcgatgac
     5761 catcagcgtg gcgttcggca cgtctacgcc gacctcgatc accgtggttg cgaccagcag
     5821 ccgcgtgcgg ccctccttga agtccatcat ggccttggct ttctgctcgc cggtcatccg
     5881 accatgaagc aggcctatcg ggggctcgag gcgctcggcg agcagctcgg cagtgggttc
     5941 cgcagcggca gcgtcgagct gctcggaatc ctcgatgagg gggcataccc agtagacctg
     6001 ctcaccccga gccatcgcat cggctgcgct tcgaatcact tcctccttgc gggcgtcgct
     6061 gatcacacgg gtgtcgatgg gcgtccgccc cggcggaagc tcgtcgagaa cggagacgtc
     6121 catgtccgcg tacagggcca tggtcaacgt gcgcggaatc ggcgtggcgg tcatgaccag
     6181 ctgatggggc gcggccccct tgctgcgcag cgccatgcgc tggtgcacgc cgaaccgatg
     6241 ctgctcgtcg acgatggtca gcgccagggc gttgaagttc accttgtcct ggaacagggc
     6301 atgggtgccc accgccacca gcgcttcgcc ctcctctagc gcctgctgcc ttgcggcacg
     6361 ttctttcgcc gactgggaac ccgtgagcaa cacgacgggg atcccgagcg gttcgagcca
     6421 ggcggagaag ttcacgtagt gctgctccgc aaggatttcg gtcggcgcca tcaatgccgt
     6481 ctggcggccg tgctcggctg ccctaatggc tgcgaaagcg gcgaccacgg tcttgccgga
     6541 gccgacgtcg ccttgcagaa gccgcagcat gggggtttcg ctggacaggt cctccagcac
     6601 ctccgtaatc acccttcgtt gggcatgggt gagcctgaat ccgagcctgc gcaagagctc
     6661 ccggccgagc tgctgggatt ggggcagcgg cagcgcgcgg gatttggcgc gatcgcgctg
     6721 gcgctgcttc atgaccaggt agtaggcggt gagctcgtcc agggcgaccc tctcctgggc
     6781 tcgttcgatg tcggcggcgg gcgcgtccgc cggcggccga tgcagatagg cgagggtctg
     6841 gtatggcgcg ccggagtcgc tgggccattc caaggaaagg gcctgatcga tcagcccgcg
     6901 caggcggttc tgccctagac ccttggtcgt ggggtagacg ggcgttagct ccggatctgg
     6961 acccgggggc tcggaggtaa aggcgcggta ctccgggtgc gccatctcca gcccgggacc
     7021 gccgccccgg gggccgaaac ggatctcgcc gaagcaacgc acgtacgtcc ccggctgcag
     7081 cccggactgt tgacgctttg agaagtgaaa gaatctcagg cccaggtagc cggtgccgtc
     7141 ctcgatggtc accagccagc tgcgacggcg accgtagccg agggatgaat ccaccacctg
     7201 cccctgcacc aggcactcct gttgtgggtg gacgtcgcga agggggacca cgcggctgcg
     7261 atcctggtag cgaatgggca ggtgcagcag caggtcgccg acccggaaaa tgcccaggcg
     7321 ctcgagcttc gccttcaagc tcggtccgac acctttcagg gattccacgc gctgttcagc
     7381 gatcacggcg cctgccg
//
LOCUS       DBNHOX010000047         3125 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_197319, whole genome shotgun sequence.
ACCESSION   DBNHOX010000047 DBNHOX010000000
VERSION     DBNHOX010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_197319"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="AC16UZ_01010"
     CDS             complement(<1..458)
                     /locus_tag="AC16UZ_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014065.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HIR6245285.1"
                     /translation="MAVNVPPLQELLVVPGVSLGTAGAAIKRQGRDDIAVLLLHPQTR
                     AAGVFTRSSFRAAPVTLADRRLSGGRARALVINSGNANAATGDPGLADAVAVCDAVAA
                     ALDVPGDSVLPFSTGVIGERLPVSRMLPAVVSACGSLAPDGWEAAARAIMT"
     gene            complement(472..>3125)
                     /gene="secA"
                     /locus_tag="AC16UZ_01015"
     CDS             complement(472..>3125)
                     /gene="secA"
                     /locus_tag="AC16UZ_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR6245286.1"
                     /translation="NALEADLLEISDDELKATTGRLKERLEQGASLEDLLPEAFAAVR
                     ESARRAKDMRHFDVPLIGGITLHEGRIAEMRTGEGKTLVATLAAYLNALAGRGVHVVT
                     VNDYLARRDAEWMGPIYETLGLTVGVIQSGQAPQEKREAYQSDVTYGTNNEFGFDYLR
                     DNMAFSLADKFQRDLGYAIVDEVDSILIDEARTPLIISGPAEQSTELYQKIDRIAPKL
                     KQQPPVEDPEAEPTGDFVVDEKNRQVELTEVGHEKVEAELMRLGLLEEGDSLYAAANL
                     NLLHHVHAALKAHALFKRDVDYMVANGEVVIVDEHTGRAMPGRRWSEGIHQAIEAKER
                     VAIQNETQTLASTTFQNYFRLYDKLAGMTGTADTEAFEFRQIYGLDVVVIPTHMPMVR
                     EDNNDLVYLTIEEKYDAIVEDINDCIERGQPVLVGTASIESSERLSGELDRRKIAHNV
                     LNAKQHEREADIIAQAGSPGAVTIATNMAGRGTDIVLGGNWEAEAAQLDSPTDEELQR
                     LRGEWQKRHDAVIAAGGLHIIGTERHESRRIDNQLRGRSGRQGDPGSSRFYLSMEDNL
                     MRIFASDRIRGIMQSIGLEKGEAIEHRMVNNAIEKAQRKVEGHNFDIRKNLLEFDDVA
                     NDQRQVIYQQRRELMVADDIAADIEGLREEVVLDLIAEYIPPQSIEEQWDVDGLEQAL
                     QTEFASRQPVRGWLDEDDALNEDSLSRKILAEVEAEYRAKEQDWASHGIDMRVVEKQI
                     MLQVLDQRWKEHLAAMDYLRQSIHLRAYAQKQPKQEYKRESFQLFQELLENIKRDVIR
                     LLCRVQIETSDELEEAERRRREEAERRMRYTHAESSGLDGGEQEQPDDGAGKPETFVR
                     SERKVGRNEPCPCGSGKKYKQCHGKAA"
BASE COUNT          489 a         1057 c          964 g          615 t
ORIGIN      
        1 gtcatgatgg cgcgagcggc ggcctcccag ccgtccggcg ccaggctccc acaggcggaa
       61 acgaccgccg gcagcattct ggaaacgggg agacgctcac cgatgacccc ggtggagaac
      121 ggcagcacgg aatcaccggg cacatccagg gcggcggcaa ccgcatcgca gacggcaacg
      181 gcatcggcaa gaccgggatc cccggtggcc gcgttggcgt taccgctgtt gatcaccaga
      241 gctcgcgcgc gcccgccgga aagtcgccga tcggccaggg tcacgggagc ggctcgaaac
      301 gagctgcgcg tgaacacgcc cgccgccctg gtctgggggt gcagcagcag gacggcgatg
      361 tcgtcacggc cctgtctctt gatggcagcc cctgcagtac ccaaagacac accggggacc
      421 accagcagtt cctgcagcgg aggaacgttc accgccatgt cgggcttccc cctacgcagc
      481 cttgccgtga cactgtttgt acttcttgcc cgagccgcac gggcaaggct cgttgcggcc
      541 caccttccgc tcactcctca cgaaggtctc gggtttgccg gcgccatcgt ccggctgctc
      601 ctgctcaccc ccgtcgagcc cgctggactc ggcatgggtg taacgcatgc gacgttcggc
      661 ctcttcgcgc cgacggcgct cggcttcctc caactcgtcg gaggtctcga tctgcacccg
      721 gcagagcagg cggatcacgt cccgcttgat gttctccagc agttcctgga acagctgaaa
      781 gctctcccgc ttgtattcct gcttgggctg cttctgggcg taggcacgca gatgaatgct
      841 ctggcgcagg tagtccatgg ccgccagatg ctccttccag cgctggtcca gaacctgcag
      901 catgatctgc ttctcgacca ccctcatgtc gatcccgtgg gacgcccaat cctgctcctt
      961 ggcgcgatac tccgcctcga cctcagccag gatcttgcgc gacaacgaat cctcgttgag
     1021 cgcgtcgtcc tcgtcgagcc agcctctcac cggctgcctg ctggcaaact cggtctgcag
     1081 ggcctgctcc aggccatcga cgtcccactg ctcctcgatg ctctgcggag ggatgtactc
     1141 tgcgatcagg tccagaacga cctcttcgcg aagcccttcg atgtcggccg cgatgtcgtc
     1201 cgccaccatc agctcccgtc gctgctgata gatcacctgg cgctgatcgt tggcaacgtc
     1261 gtcgaactcc agaaggttct tgcggatgtc gaagttgtgg ccctcgacct tccgctgcgc
     1321 tttctcgatg gcgttgttca ccatgcgatg ctcgatggcc tctcccttct cgagcccgat
     1381 ggactgcatg attcccctga tgcggtccga tgcgaatatc cgcatcaggt tgtcttccat
     1441 ggaaagatag aagcgggacg agcccgggtc gccctgacgc cccgaccggc cgcggagctg
     1501 gttgtctatg cgccgcgact cgtggcgctc ggtgccgatg atgtgcaggc caccggcagc
     1561 aatgaccgca tcgtggcgct tctgccactc gccgcgcagg cgctgcagct cctcatcggt
     1621 aggcgaatcc agctgggccg cctcggcttc ccagttgccg ccgagcacga tgtccgtgcc
     1681 tcgaccggcc atgttcgtgg cgatggtcac cgcgcccggg ctgccggcct gggcaatgat
     1741 gtcggcctcg cgctcgtgct gcttggcgtt gagcacgttg tgagcgatct tccggcgatc
     1801 aagctcccca gacagccgct cggaagattc gatggaggcg gtccccacca gcaccggctg
     1861 gccgcgctcg atgcagtcgt tgatgtcctc gacgatcgcg tcgtactttt cctcgatggt
     1921 gaggtaaacc agatcgttgt tgtcttcccg aaccatgggc atgtgggtgg ggatcacgac
     1981 gacgtcgagg ccgtatatct gcctgaactc gaacgcctcg gtgtcggcgg tgcccgtcat
     2041 gccggcaagc ttgtcgtaga gccggaagta gttctggaac gtggtcgagg ccagggtctg
     2101 ggtctcgttc tgaatcgcca cccgttcctt ggcctcgatg gcctgatgaa tgccctcgga
     2161 ccagcgcctg cccggcatgg cccgaccagt atgctcatcg acgatgacca cctcaccgtt
     2221 ggccaccatg taatccacgt cccgcttgaa cagggcgtgt gccttgagtg ccgcatgcac
     2281 gtgatgcagc aggttcaggt ttgcggccgc gtaaaggctg tcgccttcct cgaggaggcc
     2341 gagccgcatg agctccgcct ccaccttctc atggccgacc tcggtcagct ccacctggcg
     2401 gtttttctcg tccaccacga aatcgcccgt cggctcggct tccggatcct cgaccggcgg
     2461 ttgctgcttg agcttgggcg cgatccggtc gatcttctga taaagctccg tgctctgctc
     2521 ggcggggccg gagatgatga gcggcgtgcg ggcctcgtcg atgagaatgg agtccacctc
     2581 gtcgacaatg gcgtaaccga ggtcacgctg aaacttgtcg gccagcgaga acgccatgtt
     2641 gtcgcgcagg taatcgaagc cgaactcgtt gttcgtgccg taggtcacgt ccgactgata
     2701 ggcttcccgc ttctcttgcg gcgcctgacc cgactggatc acgcccaccg tgaggccgag
     2761 cgtctcgtag atcggtccca tccactccgc gtcgcgtcgt gcaaggtagt cgttgacggt
     2821 caccacgtgc accccgcgac cggccagggc gttgaggtag gcggccagcg tcgccaccag
     2881 ggtcttgccc tcgccggtgc gcatctccgc gatgcgcccc tcgtggaggg tgatcccacc
     2941 aatcagcggg acgtcgaaat ggcgcatgtc cttggcgcgg cgggcagact cacgcaccgc
     3001 ggcaaacgct tccgggagca ggtcctccag actggcgccc tgctccagcc gttccttcag
     3061 ccggccggtg gtggccttga gttcgtcgtc ggaaatctcg agcaggtcgg cctcgagcgc
     3121 gttga
//
LOCUS       DBNHOX010000048         6835 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_647588, whole genome shotgun sequence.
ACCESSION   DBNHOX010000048 DBNHOX010000000
VERSION     DBNHOX010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6835)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_647588"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="AC16UZ_01020"
     CDS             complement(<1..623)
                     /locus_tag="AC16UZ_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6245287.1"
                     /translation="MYKIRTLNNIAVRGLERFSREHYEVGSEIADPHAILLRSHQLAP
                     TDLNESLRAVARAGAGVNNVPVADCTEKGIVVFNTPGANANSVKELVLAALLISARDV
                     VGGIEFVSSLGDIEDEDELDRLVEAEKKRFKGRELQGKSLGVVGLGAIGSLVARAALD
                     LGMEVLGYDPAISVDAAWRLPSQVRRMENLPSLFARADYVTLHVPLLP"
     gene            complement(713..1780)
                     /locus_tag="AC16UZ_01025"
     CDS             complement(713..1780)
                     /locus_tag="AC16UZ_01025"
                     /inference="COORDINATES: protein motif:HMM:NF009102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR6245288.1"
                     /translation="MSHVVFTGGGTAGHVVPTFPVIAALAGRGVRISFVGSNSGLEAE
                     LLEGVDADFYGIAAGKLRRYLSVENLRDVFRVLLGVWQAFRLLGRLRPDVVFSKGGFV
                     SFPVVFAAWLRRVPVVAHESDLTPGLANRLALPFLRTVCTSFSSTAFPGFKGRVVHTG
                     TPLREALLAGDRARGRQRLGAGKDERVLLITGGSLGADVINQVVREVLDELLASWLVV
                     HVCGPGKVTGTAQPRYLQFEYVTEGWGDILTAADLVVSRAGANTLYELLALGKPNLLI
                     PLSRRASRGDQLENAEFALAQGYSRVLLEEDLDGPALLREIAALDAQREAAAARLHGF
                     APPDAVELICSELTPFLPAGS"
     gene            complement(1777..1959)
                     /locus_tag="AC16UZ_01030"
     CDS             complement(1777..1959)
                     /locus_tag="AC16UZ_01030"
                     /inference="COORDINATES: protein motif:HMM:NF047237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdaA/DnaA family protein"
                     /protein_id="HIR6245289.1"
                     /translation="MRRLAKERGLELSDAVLGYWLARSHRSVRRLLDDLEVIDAEAMR
                     AQRVVTIPLLKDALGL"
     gene            complement(1969..2475)
                     /locus_tag="AC16UZ_01035"
     CDS             complement(1969..2475)
                     /locus_tag="AC16UZ_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245290.1"
                     /translation="MTGPRQQLSLPFPINQRFVLGNFEIGENAEVVQRLEAEPDRSVF
                     SGLWLWGGGAVGVSHLLHGVCHWHAARGARVAYLPLGAVERDPAVLEGLDFYDLLALD
                     DVEVWAGDLQLESPLMAMYERLFARRARLIVGAAGPAARCGFRLADLASRLSSLTAYE
                     VRPLNDEG"
     gene            complement(2489..3064)
                     /locus_tag="AC16UZ_01040"
     CDS             complement(2489..3064)
                     /locus_tag="AC16UZ_01040"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR6245291.1"
                     /translation="MFTTAQIPNLITVARIAMVAPVGLLLWYGSVREALILMAVASAS
                     DALDGWLARHFGWISRFGAAMDPVADKLLVGTLFVIFTLKGIIPIWVALIVLGRDVII
                     LGGALAYRLLFETIEFAPTYLSKANTAVQIVLLLLLLIAQCGFDWISGAAALLADPYG
                     FWVLAALGLTSGLDYVITWSLRAVRRAKAAL"
     gene            complement(3065..4138)
                     /locus_tag="AC16UZ_01045"
     CDS             complement(3065..4138)
                     /locus_tag="AC16UZ_01045"
                     /inference="COORDINATES: protein motif:HMM:NF021367.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HIR6245292.1"
                     /translation="MPTVVSARSHLLKVTLAFLLTGGAVADVVPWLYEVEVPVENQSR
                     EARLDASGEALLQVLTRVTGLTSVPRVEAVREALSAPGRYYNQFRYLEVEGVGADGRP
                     IEELKLIVQFERGSLLQLVKDAELPVWRSNRPRVAAWIVLDRGGARSILSPEDAAGLA
                     SELSARARDRGVLVSLPLMDLQDSMLVDPAAVWGRVSTVLEPASARYGADVVLVGRLQ
                     QIGAEQWTGAWDFWLDGDLHPFSMSAENAVRLVEAGVDFLADELAQRHAVLGRFARPV
                     LVGVSGVGSARDYGELLSYLAELEFVDDIAVAGVKGDRLGLVLTTRADIEQLLANFRF
                     DRRLAELPGAVPGSADVELLWRR"
     gene            4243..5283
                     /gene="purM"
                     /locus_tag="AC16UZ_01050"
     CDS             4243..5283
                     /gene="purM"
                     /locus_tag="AC16UZ_01050"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR6245293.1"
                     /translation="MTKGLTYRDAGVDIDAGNELVRRISAACKATQRPEQLTGLGGFA
                     ALAAMPERYRQPVLVTGTDGVGTKLKLAIDHDRHDQVGQDLVAMCVNDVLVTGAEPFL
                     FLDYYATGRLDVDVAERVIKGIAHGCELSGCALVGGETAEMPGFYAAGDYDLAGFCVG
                     VVEREDIVTGREIAPGDGLIGLPSSGPHSNGYSLIRRIIEERTVHVDGELLDQLMAPT
                     TIYAKQVLALAEAIPVRGMAHITGGGLLENLPRMFAAADVAALVDMDSWTRPEVFSWL
                     QQAGNVEEREMLRTFNCGIGYVICVAGEDTAAALSTLTDLGSSPTVIGRVVPAGTEAG
                     QGQLLIAPEVEP"
     gene            complement(5280..5843)
                     /gene="dcd"
                     /locus_tag="AC16UZ_01055"
     CDS             complement(5280..5843)
                     /gene="dcd"
                     /locus_tag="AC16UZ_01055"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR6245294.1"
                     /translation="MTIKSDRWIREMAAQGMIEPFEPQQVRLRGDEKVISYGTSSYGY
                     DVRCARNFKVFTNIYSATVDPKAFDERSFVDVTGDVCIVPPNSFALASTVEYFRIPED
                     VLTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTNLPAKIYANEGVAQMLFFQ
                     SDERCEITYKDRGGKYQGQRGVTLPRP"
     gene            complement(5879..>6835)
                     /gene="apbC"
                     /locus_tag="AC16UZ_01060"
     CDS             complement(5879..>6835)
                     /gene="apbC"
                     /locus_tag="AC16UZ_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HIR6245295.1"
                     /translation="SSGNQVAVTLGYPAAGIRDRLAGQLADFLGVSAVDLDLRFQAPP
                     GRGFSRVKHIIAVASGKGGVGKSTTAVNLALALDREGARTGLLDADIYGPSQGMMLGV
                     AEGRRPEVRDQQYFEPIKAHGIQAMSMSFLVNEDTPMVWRGPMVSGALQQLLNQSLWD
                     DLDYLVVDMPPGTGDIQLTLSQKVPVSGAVIVTTPQDIALLDAKKGIEMFRKVDIPVL
                     GVVENMSVFRCPHCGGESAVFGTGGGARIAAETGVPVLAELPLAMQIREQADGGLPTV
                     VQDPTGEIAGLYREAARHLAARLWGIEARADADMPEIRITDD"
BASE COUNT         1244 a         2367 c         2188 g         1036 t
ORIGIN      
        1 ggaagcagcg gaacgtgcag ggtcacgtag tcggcccgcg caaacaggct cggcaaattc
       61 tccattcggc ggacctggct tggcagacgc caggcggcat cgaccgagat ggccgggtcg
      121 tagcccagca cctccatgcc gagatcgagg gccgcacgag ccaccagcga gccgatggcg
      181 ccaagcccca cgacgccgag tgacttgccc tgcagctcgc gccccttgaa ccgctttttc
      241 tcggcttcga caagccgatc gagctcgtct tcgtcctcga tatcgcccag cgagctgacg
      301 aactcgatgc cgcccaccac gtcccgcgcg gagatcagca gagcggccag aaccagctcc
      361 ttgaccgagt tcgcgttggc accgggcgtg ttgaacacca cgatgccctt ttccgtgcag
      421 tccgccaccg ggacgttatt gaccccggca cccgcccgcg ctaccgcgcg caggctctcg
      481 ttgaggtcgg tcggggccag ctggtggctg cgcagaagaa tggcgtgcgg gtccgcgatc
      541 tccgagccca cctcgtagtg ctcgcgggaa aatctttcca gcccgcgaac ggcaatgttg
      601 tttaatgtcc gaatcttgta catacgctgc agccgctgtc ccgttgccca tcacccccgg
      661 aggcgggggg cgcgtatggt atacaaatcc tgttgccgcg cggaaggtcc gatcaggatc
      721 cggccggaag aaagggggtc agctccgagc agatcagctc caccgcgtca ggcggcgcaa
      781 agccatgcag ccgggccgcc gcggcttcac gttgcgcatc cagcgcggcg atctctcgca
      841 gcagcgcggg gccgtccaga tcttcctcca gcagcacccg gctgtaaccc tgggccaggg
      901 caaactcggc gttctccagc tgatcgcccc tgctggcccg ccgggaaagg gggatcagca
      961 gattcggctt gccgagagcc agcagctcat agagcgtgtt ggcccccgca cgggagacca
     1021 ccaggtctgc ggccgtcagg atgtcgcccc agccctcggt gacgtactcg aactgcaggt
     1081 agcgcggttg cgctgtgccc gtgacctttc ccggcccaca cacgtggacc accagccagc
     1141 tcgccagcag ctcgtcgagc acctcgcgaa ccacctgatt gatgacgtcc gcgccgaggc
     1201 tgcccccggt gatcagcagc acgcgctcgt cttttcccgc cccgagccgc tgccggcccc
     1261 gggcgcggtc cccggccagg agcgcttccc gcaggggtgt ccccgtatgc accacccgcc
     1321 ctttgaagcc gggaaacgcc gtcgaggaaa agctggtgca gacggttcta aggaacggca
     1381 gggccaggcg gttggccaga ccgggcgtga ggtcggattc gtgggcaacg acgggcacgc
     1441 gccgcagcca ggcggcgaaa accactggaa aactgacgaa gccgcctttg gagaacacca
     1501 cgtcgggacg cagtcggccg agcagcctga atgcctgcca gacccccagc agcacgcgaa
     1561 agacgtcgcg aagattctcg acagacaggt agcggcgcag cttgcccgcg gcgatgccgt
     1621 agaagtcggc atcgacgcct tcgagcagtt ccgcttccag cccgctgttg ctgccaacga
     1681 agcttatccg aacgccgcgg ccggcaagcg cggcgatcac ggggaaggtg ggcaccacgt
     1741 gaccggccgt tccgccaccg gtgaagacca cgtggctcac aggcccagcg cgtccttgag
     1801 caggggaatg gtcacgaccc tctgagcgcg catggcctcc gcatcgatga cttccaggtc
     1861 atcgagcagg cgccgaaccg agcggtggct gcgcgcaagc cagtagccga gcaccgcatc
     1921 ggacagctcc agcccccgct ccttggccaa ccgccgcagg agacgtacct agccctcgtc
     1981 gttcaggggc ctgacctcgt atgcggtcag gctcgacagg cgcgaagcga ggtcggcgag
     2041 ccggaagccg catcgagctg ccggacccgc cgccccgacg atcaacctgg ctcgccgcgc
     2101 gaacagccgc tcgtacatcg ccatcagcgg gctctcgagc tgcagatcac cggcccagac
     2161 ctcgacgtcg tcgagggcca gaagatcata gaaatccagt ccttccagga cggcgggatc
     2221 acgctcgacg gcccccaggg gcagataggc aacccgcgcg ccgcgcgccg cgtgccaatg
     2281 acacacgcca tgcagcagat ggctgacacc caccgccccg ccgccccaga gccatagacc
     2341 cgagaagacg ctccgatccg gctccgcctc gagtcgctgg acgacctcgg cattttctcc
     2401 gatctcgaag ttgcccagca cgaatcgctg attgatggga aacggcagcg acagctgctg
     2461 tcgcggaccc gtcatcgtcc cggccgactc acagcgcggc cttggcccgg cgcacggctc
     2521 gcaggctcca agtgatcacg tagtccagcc ccgacgtgag cccgagggcg gcaaggaccc
     2581 agaagccgta cggatcggcc agcagcgccg ccgccccgct gatccagtcg aagccgcatt
     2641 gcgcgatgag cagcagcaga agcaggacga tctgcacggc ggtattcgct ttgctgaggt
     2701 aggtgggggc aaattcgatg gtctcgaaaa gcaggcggta ggcaagggcc ccgcccagga
     2761 tgatcacgtc gcggcccagg acgatcaggg caacccagat cggtatgatc cccttgaggg
     2821 tgaagatcac gaacagcgtg cccaccagca gcttgtccgc aaccggatcc atggcggcgc
     2881 caaagcggct aatccaaccg aagtgtcggg ccagccatcc gtccagcgca tcggacgcgc
     2941 tggcgaccgc catcagaatc agcgcctccc ggaccgaccc gtaccagagc aacaggccga
     3001 cgggagcgac catcgcgatg cgcgccacgg tgatgaggtt ggggatctgg gccgtcgtga
     3061 acattcagcg tcgccagagc agctcgacgt ccgccgagcc cggcaccgcg cccggcagct
     3121 cggcgagccg ccggtcgaag cgaaagttcg caagcagctg ttcgatgtcg gcgcgagtgg
     3181 tgagcaccag tccgaggcga tcacccttga cgccggccac tgcgatgtcg tcgacgaatt
     3241 ccagctctgc cagatagctg agcagctccc cgtagtctct ggccgagccg acgccggaca
     3301 cgccgacgag caccggccgg gcaaacctgc cgaggacggc atgccgctgc gcgagctcgt
     3361 cggcaagaaa gtccacgccc gcctccacca gccggacggc attttcggca ctcatcgaaa
     3421 acggatgcag atcgccatcg agccagaaat cccatgcgcc cgtccactgc tcggcgccga
     3481 tctgctgcag acgcccgacg aggacgacgt ccgcgccgta gcgggccgag gccggctcga
     3541 gcaccgtaga tacccgcccc caaaccgcgg cgggatccac gagcatactg tcctgaaggt
     3601 ccatcagcgg cagcgacacc agcacccccc ggtcccgggc ccgcgccgac agctcggaag
     3661 cgaggccggc cgcatcttcc ggagagagga tggaacgtgc cccgccgcgg tccagcacga
     3721 tccaggccgc cacccgagga cggttggaac gccagacggg cagctcggca tccttgacca
     3781 gctgcagcag agatccgcgc tcgaactgca cgatgagctt gagctcttcg atcggccgtc
     3841 cgtccgcgcc cacaccctcc acctcgaggt agcggaactg gttgtagtag cgccccggag
     3901 ccgacagcgc ttcccgaacc gcctccacgc ggggcacgct ggtcagaccc gtcacgcggg
     3961 tgagcacctg cagcagggct tcgccggagg cgtcgagcct tgcctcgcgg ctctggttct
     4021 ccaccgggac ctctacctcg tagagccagg gaacgacgtc cgcgacggcg ccgcccgtca
     4081 gcaggaacgc aagcgtgact ttcaggaggt gcgatcgtgc tgagaccacc gtgggcaccg
     4141 cggctccgac atcagtacgg cgtcgaagtt tacccaattc gccaggtcag gtctggtaaa
     4201 ctccgccgcc atcaaggggc ggcaacaggg cgaggattca acgtgaccaa gggtctcacc
     4261 taccgtgacg ccggggtgga catcgacgcc ggcaacgagc tcgtacgccg aatctcagcc
     4321 gcctgcaaag cgacgcagcg acccgagcag ctcaccggcc tcggcggctt cgcggccctg
     4381 gccgcaatgc ccgagcgtta ccggcaaccg gtcctggtga cgggcaccga cggtgtgggc
     4441 accaagctca agctcgccat cgatcacgac cggcacgacc aggtcggcca ggacctggtg
     4501 gccatgtgcg tgaacgacgt tctggtgacc ggtgccgagc cgtttctttt cctggactac
     4561 tacgccaccg gccgactgga cgtggacgtg gcggaacggg tcatcaaagg tatcgcccat
     4621 ggctgcgagc tgtccggctg cgccctggtg ggcggcgaga ccgccgaaat gcccgggttt
     4681 tacgcggccg gcgactacga cctggccggg ttctgcgtcg gggtcgtcga gcgcgaggac
     4741 atcgtcaccg gcagagagat cgcgcctggc gacgggttga tcgggctgcc gagcagcggc
     4801 ccccattcca atggatactc gctcatccgt cggatcatcg aggagcgaac cgtccacgtg
     4861 gatggtgagc tgctagacca gctcatggcg ccgaccacca tctacgccaa gcaggtgctc
     4921 gcgctggcag aagccatccc cgtgcgcggc atggcccaca tcaccggcgg cggccttctc
     4981 gagaacctgc cccgaatgtt cgccgccgcc gacgttgcgg cgctggtgga catggactcg
     5041 tggacccggc cggaggtgtt cagctggctg cagcaggccg gcaacgtcga ggagcgggag
     5101 atgctgagaa cgttcaactg cggcatcggc tacgtgatct gcgttgccgg agaggacacc
     5161 gcagcagccc tgtcgaccct gacggatctg ggttcttcgc ccaccgtcat cggccgcgtg
     5221 gtcccggcgg gaaccgaggc cggtcagggt cagcttctca tcgcgcccga ggtcgagccc
     5281 tagggtctgg gcagggtcac gccgcgctga ccctgatact tgccgccgcg gtccttgtag
     5341 gtgatctcgc agcgctcgtc agactgaaaa aagagcatct gcgctacacc ttcgttggca
     5401 tagatcttcg ccggcaggtt ggtcgtgttg gagaactcca gcgtgacgtg gccttcccac
     5461 tcgggttcga gcggggtgac gttgacgatg atgccgcagc gggcgtaggt cgacttgccc
     5521 agacagatgg tgagcacatc ctcgggaatc cggaagtact ctaccgtgct ggccagcgcg
     5581 aacgagttcg ggggaacgat gcagacgtcc ccggtcacgt ccacgaagct tcgctcgtcg
     5641 aacgccttgg ggtcgacggt ggcggagtag atgttcgtga agaccttgaa gttccgcgcg
     5701 cagcggacat cgtagccata gctggacgtc ccgtaggaaa tgaccttttc atcgcccctg
     5761 aggcgcacct gctgcggctc gaagggttcg atcatgccct gcgccgccat ctcgcgaatc
     5821 cagcgatccg acttgatcgt caccgtgcca ccccgatctg ctgtggttgg gccatggtct
     5881 agtcgtccgt gatgcggatc tcgggcatgt cggcgtccgc acgtgcctca atgccccaca
     5941 gccgcgccgc gagatgccgc gcggcctccc ggtacagacc ggcaatctcg cccgtcggat
     6001 cctgcacaac ggtcggcagg ccaccgtccg cctgctcccg gatctgcatg gccagcggca
     6061 gctcggcaag cacgggcaca ccggtctcgg cagcaatccg cgccccgcct ccggtaccga
     6121 acacggcgct ctccccgccg cagtgcgggc agcggaagac gctcatgttt tcgaccacgc
     6181 cgagcacggg aatgtcgacc ttgcggaaca tctcgatgcc tttcttggca tccagcaggg
     6241 cgatgtcctg aggcgtggtg acgatcacgg cgcccgacac gggcaccttc tgggacagcg
     6301 tcagctggat gtcgccggtt cccggcggca tatccaccac gaggtagtcc agatcgtccc
     6361 acagcgactg gttgagcagc tgctgcaacg cgccggaaac catgggcccg cgccagacca
     6421 tgggcgtgtc ctcgttcacc aggaagctca tggacatggc ctggattcca tgggccttga
     6481 tgggctcgaa gtactgctgg tccctgacct cgggccgccg accctcggcg acgccgagca
     6541 tcatcccctg gctggggccg taaatgtcgg cgtcgagcag acccgttcgc gcgccttccc
     6601 gatccagcgc cagcgccaga ttcacggcgg tggtggactt gcctacgccg cccttgcccg
     6661 aggcaacggc aatgatgtgc ttgacccggc tgaatcctct ccccggaggt gcctgaaacc
     6721 gcagatcgag atcgacggca ctcaccccga gaaaatcagc cagctgcccg gcaagccggt
     6781 cgcggatacc ggccgcgggg tagcccaggg tgacagcaac ctggttgccc gagct
//
LOCUS       DBNHOX010000049         1672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_518918, whole genome shotgun sequence.
ACCESSION   DBNHOX010000049 DBNHOX010000000
VERSION     DBNHOX010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_518918"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1159
                     /locus_tag="AC16UZ_01065"
     CDS             152..1159
                     /locus_tag="AC16UZ_01065"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR6245296.1"
                     /translation="MESRSIPTELAQWLAGELARLDLDPTFTSQALLVEASYRCFYRI
                     RSRHRGGEALSLVAMESPPERENNQQFVTLAKVFAAHGVGVPRILAADLTDGFLLMTD
                     LGDRHFADAYQSGERDRALAAGIDTLIRLQTVDDPAVPLYTARRFRDELGIFREWFVE
                     RWLGEPFPAEDLAGIPERLVACVEQQLQVCVHRDFHSRNLLLAADGSVGVVDFQDALI
                     GPVSYDLASLLRDCYYRFPEDEIARWRDYYLERTPHSLDPEQFARDLDLTAAQRQLKA
                     VGIFARLDLRDGKTSHLRHIPPVLDHLAALSARHDTLTPLGPHLAVWQKAAERRLQAR
                     R"
     gene            1156..>1672
                     /locus_tag="AC16UZ_01070"
     CDS             1156..>1672
                     /locus_tag="AC16UZ_01070"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR6245297.1"
                     /translation="MRAMILAAGRGERLKPLTDAVPKPMLTVAGEPLIARQLRALGEA
                     GITRVVINLHHLGEQIASFCGSGKDYGVAIDYSREDELLDTGGGIVKALPLLGDAPFL
                     ILNGDILTDFPFQTLATALPDWADLHLVLTSRPAHRTHGDFDWEAGRVTGRGESYVYC
                     GIAVLRPELFDG"
BASE COUNT          257 a          602 c          542 g          271 t
ORIGIN      
        1 cgtccactgc agccgacgcc ccggtgagca gtccggccag gagcaggcag catccggcac
       61 ggcgtcgtga ggaagggcgg ctcgtcatgg ccgcggatga taaaagattt acctccccgg
      121 gaataacggc ggccgcgcta caattgcccg ggtggaatcc cgcagcatac caaccgaact
      181 cgcgcagtgg ctcgccggtg agctggctcg cctggacctc gacccgacgt ttaccagcca
      241 ggcgctcctg gtcgaggcga gctaccgctg cttctatcgg atccgctccc ggcatcgcgg
      301 cggggaggcg ctgagcctgg tggccatgga atcgcctccc gagcgcgaga acaaccagca
      361 gttcgtcacc ctggccaagg tcttcgccgc ccatggcgtg ggggttccgc gcattctcgc
      421 cgccgatctc acggacggtt tcctgctgat gaccgactta ggcgatcggc acttcgcaga
      481 cgcctaccag agcggcgagc gcgatcgggc cctggccgcc ggcatcgaca cgctcatccg
      541 ccttcagacc gttgacgacc ccgccgtgcc gctctacacg gctcggcggt tccgggacga
      601 gctcggcatc ttccgggagt ggttcgtgga gcgctggctc ggcgagccgt ttcctgccga
      661 ggacctggcc ggcattccgg agaggctcgt ggcgtgcgtc gagcagcagc tgcaggtctg
      721 cgtccaccgg gacttccaca gccgcaacct gctcctggca gccgatggca gcgtgggcgt
      781 cgtcgacttc caggacgctc tgatcgggcc cgtgagctac gacctggcct cgctgctgcg
      841 ggactgctac taccgctttc ccgaggacga gatcgcgcgg tggcgggatt actatctgga
      901 acggaccccc cactcgctgg accccgagca gttcgctcgc gacctggacc tcaccgccgc
      961 ccagcggcag ctgaaagcgg tggggatctt cgcccgtctc gacctcaggg acggcaagac
     1021 gtcgcatctt cgacacatcc cgcccgtgct cgaccatctc gctgcgctgt cggcccggca
     1081 cgacacgctc acgcccctcg gtccccatct ggccgtctgg cagaaggccg ctgaacgccg
     1141 tctgcaggcc cgccgatgag agcgatgatc ctggccgccg gccggggcga gcggctcaag
     1201 ccgctcacgg acgccgtgcc gaaacccatg ctgacggtgg ccggcgagcc gctcatcgcc
     1261 cggcagctgc gcgccctggg agaggccgga attacccggg tggtgatcaa cctgcaccac
     1321 ctaggcgagc agatcgcgtc gttctgcggt tccgggaaag actacggggt ggccatcgac
     1381 tacagccgcg aggacgagct gctggacacc ggcggcggca tcgtcaaggc gctgcccctg
     1441 ctgggcgatg cgcccttcct gattctcaac ggcgacatcc tcaccgactt ccccttccag
     1501 acgctcgcca ccgccctgcc cgactgggcc gacctgcatc tggtgctcac gtcccgaccg
     1561 gcccatcgta cccacggcga tttcgattgg gaggcgggca gggtcaccgg gcgcggcgag
     1621 tcctacgtct actgcggcat cgccgtcttg aggcccgagc tcttcgacgg cc
//
LOCUS       DBNHOX010000050         3908 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1097466, whole genome shotgun sequence.
ACCESSION   DBNHOX010000050 DBNHOX010000000
VERSION     DBNHOX010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1097466"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="AC16UZ_01075"
     CDS             complement(<1..375)
                     /locus_tag="AC16UZ_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245298.1"
                     /translation="MALGDRHYRWVVVGYTLVIQGVSIGMLVYSFALFAIPWLDLFAA
                     PRRDVMLTVAILQLVNGAVSPLAGRAMDRYSIRTIVLAGLALMLVGLVVVSRAQALWQ
                     VWLVYATLFPVSLAMMSTLASQT"
     gene            complement(377..1552)
                     /locus_tag="AC16UZ_01080"
     CDS             complement(377..1552)
                     /locus_tag="AC16UZ_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6245299.1"
                     /translation="MSAFRDDVRGWLEENCPPSMRTPLPADEYPGGGRRAVYGNPDTG
                     VWMQRMADRGFPVPLWPTEYGGAGLDAGENQVLQEELRRINARPALLGMGISMIGPAL
                     LEFGTEAQKREHLPKIARGEIWWCQGYSEPNAGSDLAGLGTSAVEDGDDYIVNGQKIW
                     TSGADRADWIFCLVRTDPSAPKHEGITFILFDMASPGVTVKPIELISGYSPFCETFFD
                     NVRASRRNVVGEPNKGWTVAKRLLQYERTSIGGIGGSSQKAATLEEIAREALGTVDGR
                     IADADLRAGILRHRMNDRAFGLTMKRSVQQTAAGIAPGAESSFFKYYGTEQNKGRYEL
                     LMSLLGTRGLGWSGPGFSDEERDATRAWLRSKANSIEGGTSEVQLNVIAKRILGLPD"
     gene            1694..3004
                     /locus_tag="AC16UZ_01085"
     CDS             1694..3004
                     /locus_tag="AC16UZ_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012614627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR6245300.1"
                     /translation="MSESTDRPATDDTHEFGFHTRSVHAGSRPDPATGARALPIYQTS
                     SYVFEDSESAAAYFNLQEYGNTYSRIMNPTVAALEERIANLEGGAGAVAFASGLAAQS
                     AAFFTLLEPGDHVVASAALYGGTVTQLKHLFRKMSVALTFVDPDRIDTWEAALQPDTK
                     LLFAETIGNPGGNVLDIEPLAELAHRHGMPLMVDNTFATPYLTRPMEWGADIVVHSAT
                     KFLGGHGTSIGGLVVDSGEFDWSNGRFPVIAEPSPAYHGLAFHETFGTYGYLMKLRAE
                     TLRDLGAAMSPFNAFLFAQGLETLPLRMRQHVANAEAVAAYLDGREDVERVSYPGLPG
                     SPYRDRVARYLPRGAGAIFCFDLPGGREAGQRFIEQLTLFSHLANVGDAKSLVIHPAT
                     TTHRQLSDEELAAAGIGPGTIRVSIGIEDADDIIWDLEQGLAGI"
     gene            3006..3491
                     /locus_tag="AC16UZ_01090"
     CDS             3006..3491
                     /locus_tag="AC16UZ_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR6245301.1"
                     /translation="MTEHDMERYQDPHVIQGALNQRRIAVVGLSGNPLRASNFVGYYM
                     KRHGYEVVPVNPREEEILGLKSYPDLAAVPEPVGVVDVFRDPSAVPDIARAAVAAGAK
                     YLWLQFGVISFEGAEIAEAGGMQVIMDRCIKIEHARYRGRMHWLGFNTNQISAQRRRA
                     D"
     gene            3622..>3908
                     /locus_tag="AC16UZ_01095"
     CDS             3622..>3908
                     /locus_tag="AC16UZ_01095"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR6245302.1"
                     /translation="MFRKVKGGTRMAPAMGERDEIRGELTHFSPADAANEATIGATIV
                     IKGEITGDENLVIEGTLDGTANLSDRNLTIAQTGRILGNVSANSITVEGEI"
BASE COUNT          694 a         1331 c         1281 g          602 t
ORIGIN      
        1 ggtctgggaa gccagcgtgc tcatcatggc cagggagacg gggaagagcg tcgcgtagac
       61 gagccagacc tgccacaggg cctgagcccg ggaaaccacg acgagcccga cgagcatcag
      121 cgccaggccc gccagcacga tggttcggat ggagtatcga tccatggcac ggcccgccag
      181 agggctgacc gcgccgttga ccagctgcag gatcgccacg gtgagcataa cgtcgcgacg
      241 cggggcggcg aagaggtcga gccagggaat ggcgaagagc gcaaagctgt agaccagcat
      301 cccgatgctg accccctgta tcaccagggt gtagccgacg actacccagc ggtaatggcg
      361 gtcgcccagc gccacgtcag tcgggcaggc cgaggattcg tttggcgatg acgttcagct
      421 gcacctccga ggtgccgccc tcgatggagt tggccttcga ccggagccac gcgcgcgtgg
      481 cgtcgcgctc ctcgtcgctg aacccgggac cgctccagcc caggccacgc gtgccgagca
      541 gggacatcag cagctcgtag cggcccttgt tctgctcggt tccgtagtac ttgaagaagc
      601 tggactcggc gccgggggcg ataccggccg ccgtctgctg caccgagcgc ttcatggtca
      661 ggccgaaggc ccggtcgttc atgcgatggc gcaggatgcc agcccggagg tcggcgtcgg
      721 caatccgccc gtccacggtg cccagcgcct cccgggcaat ctcctcgagg gtcgccgcct
      781 tctggctgga gccgccgatc ccgccgattg aggtccgctc gtactgcaaa aggcgcttgg
      841 cgacggtcca gcccttgttg ggctccccca ccacgttgcg ccgcgaggca cgcacgttgt
      901 cgaagaaggt ctcacagaac ggcgagtagc cgctgatcag ctcgatgggc ttcaccgtca
      961 cgcccggcga agccatatcg aacaggatga aggtaatgcc ctcgtgcttg ggcgcgctcg
     1021 gatcggtccg gaccaggcag aagatccagt ccgcgcggtc ggctcccgag gtccagattt
     1081 tctgcccgtt gacgatgtag tcgtcgccgt cttccaccgc ggacgttccc aggccggcca
     1141 ggtccgagcc ggcgttgggc tcgctgtagc cctggcacca ccagatctcg ccccgggcga
     1201 tcttcggcag gtgctcgcgc ttctgggcct cggtgccgaa ctcgagcagc gcgggcccga
     1261 tcatgctgat gcccatgccc agcagcgcgg gccgcgcgtt gatgcggcgc agctcctcct
     1321 gcagaacctg gttctcgccg gcgtcgagcc ccgccccgcc gtactcggtg ggccacagcg
     1381 gcacggggaa gccccggtcc gccatccgct gcatccacac gccggtgtcc ggattgccgt
     1441 agacggcacg ccggccgccg ccgggatact cgtccgccgg caggggtgtg cgcatgctcg
     1501 gcgggcagtt ctcctccagc cacccacgca catcgtctct gaacgcgctc atcgctttcc
     1561 tcgtcgaccc atgaattcca ggcgagcaga cccgtcagaa cgcacgctag gcgttgcgac
     1621 gcggccggcc cgcataggtt atcttacctg cacaggcagc aggcagcgcg agcccgcgct
     1681 gcggggaggc agcatgagcg aatccacgga ccgcccggct acggacgaca cccacgagtt
     1741 cgggttccac acccggtcgg tgcatgcggg cagccggcct gatccggcca ccggcgcgcg
     1801 ggcgctgccc atctaccaga cgagcagcta cgtcttcgag gactccgagt cggcggcggc
     1861 atatttcaac ctgcaggaat acggcaacac ctactcacgg atcatgaacc ccaccgtggc
     1921 cgccctggag gagcgcatcg ccaacctgga gggaggcgcg ggcgcggtgg cctttgccag
     1981 cgggctggcc gcccagtcgg ccgccttctt caccctgctc gagcccggcg accacgtggt
     2041 tgcgtccgcc gctctgtacg gcggcacggt gactcagctc aagcacctgt tccgcaagat
     2101 gagcgtggcg ctcaccttcg tggaccccga ccgcatcgat acctgggagg cggccctgca
     2161 acccgacacc aagctgctgt ttgccgagac catcggcaac cccggcggca acgttctgga
     2221 catcgagcct ttagccgagc tcgcccaccg tcacggcatg ccgctgatgg tggacaacac
     2281 gttcgccacg ccctacctca cccgacccat ggagtggggc gccgacatcg tcgtgcactc
     2341 ggccaccaag ttcctcggcg gccacggcac gtccatcggc ggcctggtgg tggattcagg
     2401 agagttcgac tggtccaacg gccgctttcc ggtgatcgcg gagccctccc ccgcctatca
     2461 cgggctggcc ttccacgaga ccttcggcac atacgggtac ctgatgaagc tgcgggccga
     2521 gaccctgcgg gatctgggcg ccgccatgag cccgttcaac gccttcctgt tcgctcaggg
     2581 tctggaaacc ctgccgctgc ggatgcgcca gcacgtcgcg aacgccgagg ccgtcgccgc
     2641 ctacctggac ggtcgcgagg acgtcgagcg ggttagctac cccggcctgc ccggaagccc
     2701 ctatcgagac cgggtcgccc gctacctgcc gcgcggcgcc ggagccatct tctgcttcga
     2761 cctgccgggc gggcgcgagg cgggccagcg gttcatcgag cagctcacct tgttctccca
     2821 tctggcgaac gtgggcgacg cgaagagcct ggtcatccac ccggccacga ccacccaccg
     2881 ccagctgagc gacgaggaac tggcggccgc cggcatcggt ccgggcacca tacgcgtttc
     2941 catcggcatc gaggatgccg acgacatcat ctgggatctc gagcagggac tggcgggcat
     3001 ctgacatgac ggagcacgac atggaacggt atcaggaccc ccacgtgatc cagggggcgc
     3061 tcaaccagcg caggattgcc gtggtggggc tgtcgggcaa ccctctgcgg gcgagcaact
     3121 tcgtgggcta ctacatgaaa cggcacggct acgaggtggt tcccgtcaat ccccgggagg
     3181 aagagatcct cgggctgaaa agctatccag atcttgcagc cgtgccggag cccgtgggcg
     3241 tggtggacgt cttccgcgac ccgagcgcgg tgccggacat tgcgcgcgcc gcggtggcgg
     3301 ccggcgcgaa gtacctgtgg ctgcagttcg gcgtgatcag cttcgagggc gccgagatcg
     3361 ccgaagctgg cggcatgcag gtgatcatgg accgctgcat caagatcgag cacgcacgct
     3421 atcgggggcg catgcactgg ctcggcttca ataccaacca gatctccgca cagcgccgcc
     3481 gggcggactg aaatccgcag cgccgacctg gatatagcta cttgtcaatt cggccggtgc
     3541 tcagttaatc tgcacggtga aaggagcccc aatcgaacaa aagaaagggg gcatggggaa
     3601 ttgggacacg aggccgaagg catgtttaga aaggtcaaag ggggcacccg catggcaccc
     3661 gccatggggg aacgggacga gattcgcgga gagctaacgc acttcagccc tgcagacgca
     3721 gcaaacgagg ccacgatcgg agcaacgatc gtgatcaaag gagagatcac gggcgacgag
     3781 aacctggtca tcgaggggac cctggacggt accgccaacc tctcggaccg caatctcacc
     3841 atcgcccaga cgggtcggat tctcggcaac gtcagcgcca actcgattac cgtcgagggg
     3901 gaaatcca
//
LOCUS       DBNHOX010000051         2746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_454631, whole genome shotgun sequence.
ACCESSION   DBNHOX010000051 DBNHOX010000000
VERSION     DBNHOX010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_454631"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="AC16UZ_01100"
     CDS             complement(<1..1423)
                     /locus_tag="AC16UZ_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR6245303.1"
                     /translation="MSALPAFLLQDEEGNAHTFPGSRGALVCFVKEDCPTCNEVMPVL
                     DALNRSVDGALDMLVAGQTREGNRRLKEQHGPGFPILDDSTLKVSFAFDIDTVPTLFL
                     CDAQGVPERTLVGFVRDEWRELAELLADRAGASAPSIDWDALPEWRPGCGSLSVDPVI
                     ADRLRAEAENGPLRARRIEIASQDDEFEFMFDQGFSDGLPLIPPTPERVVRMLSGTSR
                     DPQEVVGVVAPNLGEATVEKVAINAVMAGCRPEYMPVVLAAMEAVCTDEYNIHGVMAT
                     TMGASPVMVVNGPVRHRLGMNMGLGALGQGSRANATIGRAVRLAIRNIGGARPGGTER
                     STLGNPMKFTMCFAEWEERNPWSPLHVERGFAPEDSVVTVFTMTSGPTLMVDQDSRSA
                     AQLAGSLGLCLEAAFHPKAHYATEVLVVLCPEHVDTLLRDGFDKAAIRSRIQEVTARP
                     IAELAEDPVSAVGFKKAAVDAMTP"
     gene            1555..1896
                     /locus_tag="AC16UZ_01105"
     CDS             1555..1896
                     /locus_tag="AC16UZ_01105"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR6245304.1"
                     /translation="MLLRSLRVARQAFEEGTGWKLTSEGACKGEVCIPLSKPEGTVVD
                     VAELAETLNMPLVGEPERNLWALGPEAIGGRALTTALAPELTLPDLDGNEFTLSSLRG
                     QKILVYAWAPY"
     gene            1909..2652
                     /locus_tag="AC16UZ_01110"
                     /pseudo
     CDS             1909..2652
                     /locus_tag="AC16UZ_01110"
                     /inference="COORDINATES: protein motif:HMM:NF041054.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ResA-like WAxxUGC motif-containing protein"
BASE COUNT          488 a          927 c          889 g          442 t
ORIGIN      
        1 ccggcgtcat ggcgtcgacg gccgctttct tgaacccaac cgcggaaacc ggatcttccg
       61 ccagctccgc gatgggccgg gcggtcactt cctgaatccg gctgcggatc gccgccttgt
      121 cgaagccgtc gcgcagcagc gtatcgacgt gttccgggca gagcaccacc agcacctcgg
      181 tggcgtagtg ggctttgggg tgaaaggcgg cctccagaca aaggcccagg ctgcccgcaa
      241 gctgagccgc gctgcggctg tcctgatcga ccatgagcgt gggaccgctg gtcattgtga
      301 acaccgtcac cacggagtct tcgggagcga atccccgctc cacgtgaagc ggcgaccagg
      361 gattgcgctc ctcccattcg gcgaagcaca tggtgaactt catggggttg ccgagcgtcg
      421 accgttccgt gcctccgggc ctcgctccgc cgatgttgcg gatagccagg cgcaccgccc
      481 ggccgatggt ggcgttggcc cgcgagccct gccccagggc accgagcccc atgttcattc
      541 ccaggcggtg ccggacgggc ccgttgacca ccatcacagg gctcgcaccc atggtcgtgg
      601 ccatgacgcc gtgaatgttg tactcgtcgg tgcagacggc ctccatggct gccagcacca
      661 cgggcatgta ctcggggcgg cagcccgcca tgaccgcgtt gatggccacc ttttccaccg
      721 tggcctcgcc gaggttgggc gccaccacac ccaccacctc ctgcggatca cgggaggtgc
      781 cggaaagcat tcgaacgact cgctccggcg tcggtggtat caggggcagg ccatcgctga
      841 acccctggtc gaacatgaat tcgaactcgt cgtcctggct tgcgatctct atgcgccgcg
      901 ctctcaaggg gccgttctcc gcctcggcgc gaagacgatc ggcaatcacc gggtccaccg
      961 aaagcgaacc gcagcccggc ctccattccg gcagcgcatc ccagtcgatg ctcggcgccg
     1021 aggccccggc ccgatcggca agcagctcgg cgagttcccg ccactcgtcc cgcacgaaac
     1081 ccaccagcgt gcgctcggga acgccctggg cgtcacacag gaagagcgtg ggcaccgtat
     1141 cgatatcgaa ggcaaaagag actttgaggg tcgagtcatc gaggatggga aagccgggcc
     1201 cgtgctgctc cttcaatcgg cggttgccct ctcgggtctg acctgccacg agcatgtcga
     1261 gcgcgccgtc caccgaccgg ttgagcgcgt ccagcacggg catcacctcg ttgcaggtcg
     1321 ggcagtcctc tttgacgaag cagaccagag caccccgcga cccgggaaac gtgtgggcat
     1381 tgccctcttc gtcctgcagc agaaaggcgg gaagcgcgct cattgctcgc tcttggcgtg
     1441 atcggagccg agaagttacc gcagatcgat cattcgagaa agccgttcgc cgcgttcttg
     1501 cgccaccgcc cggcagacgg caccatcgga tcaccgaaaa ggaggacgac atccgtgctg
     1561 ctgagatcat tgcgcgttgc gcgccaggcg tttgaggaag gcaccggctg gaagctaacg
     1621 tcagaggggg cctgcaaagg ggaggtctgc atacccctgt cgaaaccgga gggcaccgtt
     1681 gtcgatgtgg ccgagctcgc cgagaccctg aacatgcccc tggtggggga accggagcgg
     1741 aatctgtggg cgctcggacc tgaggcgatc ggaggccgag cgctgaccac ggcgctggca
     1801 cccgagctga ccctgcccga cctcgacggc aacgagttca cgctgtcgtc gctgcgtggc
     1861 cagaaaatcc tcgtctacgc ctgggccccc tactgaggct gctctgcgga cctgcccgtg
     1921 tggcgggtac tgcgcgatga gctgcatccg caaggtctgg agctcgtgac cgtagggctg
     1981 gacaccctgg gcggcgaggg ttgccgggcc ttcatcgaag ctgccgaacc aacccacccg
     2041 gcactcatcg accggcatca tcggctggcc gatctgttcg gcgtcatcaa cattccgagc
     2101 agcatctgga tcgacgagga aggctggatc gtccggcctg ccgagacggc ccccgcgccg
     2161 ccgaccccgg gggaagcggc ttcgcgagag gtacccggcg aaatgccgcc acgctttgtc
     2221 gagatgatgg gggaagccgt gaaaattcag tctgatccgg tcgcgtacca tgccgcgctg
     2281 cgcgactggg tcgagaaagg cagggagagc gactacgccc tgcctgccga ggaggtggtc
     2341 gcgcggtcac gtacccgcaa tcccgagatt gcccgcgggc acgcccactt cgagctggcc
     2401 acccaactcg aaatcgacgg ccagcacgac gccgcgataa ggcacttccg ggaggcccac
     2461 cggctggtgc ccgattcctg gacctttcga cgccaggcct ggtctctgga gcccgtgggc
     2521 gaagggcctc tggcaagatt ctggcagggg cccgatcccg aggatcccca ggcgtggccc
     2581 tacgagggcg actggcttac cgacatccga gcgacgggtg cccagaacta caacgatccg
     2641 tggcgaccat gacgccccac cctctcccac tggagcaagc aacatgtcgg aactgagcga
     2701 gtttcggagc gacgtcgctg cctggctgaa cgaaaactgt ccaccc
//
LOCUS       DBNHOX010000052         4189 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1161859, whole genome shotgun sequence.
ACCESSION   DBNHOX010000052 DBNHOX010000000
VERSION     DBNHOX010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1161859"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="AC16UZ_01115"
     CDS             complement(<1..476)
                     /locus_tag="AC16UZ_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005888326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6245305.1"
                     /translation="MSSSLPSDSVGLVSPQVFAHPSGFSLRCGETLEGFELVYETYGR
                     LNAARSNAILVCHALSGSHHAAGYHSEDDKRPGWWESCIGPGKPIDTDRFFVVCPNNL
                     GGCHGSTGPTTVDPRTGEPFGPNFPVVTVEDWVDTQALLADHLGIDRFAAVVGGSLG"
     gene            complement(577..1113)
                     /locus_tag="AC16UZ_01120"
     CDS             complement(577..1113)
                     /locus_tag="AC16UZ_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR6245306.1"
                     /translation="MAEVLYYATSFILQVIAFLFVVRFLLQACRADFYNPISQGIVKA
                     TDVVLKPVRMVLPGYRNLDFAALLAALIVEVVMILALGAIADRPTGTAVQIATSGVLQ
                     MILLGLFIIKWSILIVIIASFLAPGNYHPALALLQQLTEPVLAPARKLLPPMGGLDFS
                     PILVFLILGVIERVLLQI"
     gene            complement(1144..1953)
                     /gene="proC"
                     /locus_tag="AC16UZ_01125"
     CDS             complement(1144..1953)
                     /gene="proC"
                     /locus_tag="AC16UZ_01125"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR6245307.1"
                     /translation="MSSHITFIGAGNMATSLIKGLIASGHDAATIRASDPAPEQLGKI
                     AELGVATSDDNNAQVRGADVVILAVKPQVMASVAGGLTALSTDQLLVSIAAGVPMSSL
                     AGWTSSEQPIVRCMPNTPALLGAGVTALCANERVSPAQRELAESVLATAGRTLWVSLE
                     DQLDAVTAVSGSGPAYFFYLMEAMIEAGQALGLSAETAVTLTLETARGAALMALEGPP
                     PAQLRANVTSPGGTTQAALEILDAADCRGIINAAVAAAAERSRELAIEFGR"
     gene            complement(1987..2664)
                     /locus_tag="AC16UZ_01130"
     CDS             complement(1987..2664)
                     /locus_tag="AC16UZ_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR6245308.1"
                     /translation="MHSISHNVASVRSCVVSAARAAQRDPDEVCLIAVSKRQPARAIE
                     EAHGAGLKDFGENYLQEALPKIAALGHLPLTWHFIGALQSNKTRQVARHFDWVHTVDR
                     PKIARRLNEQCPDDKTLQVCLQVNIDADPGKAGVDPAAVADLLKELVDLERICVRGLM
                     TILDPNADAPESFARMAKLFAGLAPLAAGRWDTLSMGMSNDYEQAIMAGATHVRIGTA
                     IFGAREG"
     gene            2830..3864
                     /locus_tag="AC16UZ_01135"
     CDS             2830..3864
                     /locus_tag="AC16UZ_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR6245309.1"
                     /translation="MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEH
                     QQVHSLIYEIMNDKQRKDFEEFLETDFSVEVPGVARFRVNAFNQNRGAGAVFRTIPSK
                     VLTMEDLDMGQVFRDICLVPRGLVLVTGPTGSGKSTSLAAMVDFINDHRYDHILTIED
                     PIEFVHESKKCLVNQRDVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAA
                     ETGHLVFGTLHTTSAAKTIDRVIDVFPAEEKSMVRSMLSESLQSVISQTLLKRVNGGR
                     VAAHEIMIGTAAIRNLIREDKVAQMYSAIQTGSSVGMQTLDQCLLDLVQKRTITRDSA
                     REKAKNPDDF"
     gene            3882..>4189
                     /locus_tag="AC16UZ_01140"
     CDS             3882..>4189
                     /locus_tag="AC16UZ_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HIR6245310.1"
                     /translation="MQFEKLLKLVVEKGASDLFITAGKPPMLKVNGKLFAAGTTNLSA
                     DQSRDVVHSIMDEDQKKQFHDHHECNFAIHSEGLGRFRVSAFQQRNLAGMVLRRIEVN
                     "
BASE COUNT          796 a         1283 c         1374 g          736 t
ORIGIN      
        1 cccaggctgc caccgacgac ggcggcgaag cgatcgatgc ccaggtggtc ggcgagcagg
       61 gcctgagtat ctacccagtc ttcaacggtg acgacgggaa agtttggccc gaacggttca
      121 cccgtgcggg ggtcgaccgt ggtcggcccc gtgctgccgt ggcagccgcc gagattgttg
      181 gggcagacga cgaagaaccg gtctgtgtcg attggcttgc cgggcccgat gcagctttcc
      241 caccacccgg gtcgtttgtc gtcctcggag tggtagccgg cagcgtgatg gctcccggag
      301 agagcatggc agacgagaat cgcgttgctc cgcgcagcgt tgagccgtcc gtaggtctcg
      361 tagacgagct cgaacccctc gagcgtctca ccgcagcgaa gcgaaaagcc ggagggatgc
      421 gcgaagacct gcgggctgac caggcccacg gaatcggatg gcaagctgct cgacactgag
      481 taggccacga agaacgggac ggcgagtcta aagcaagggc ttcaaggcca tcaaccgggt
      541 cggtcggggc atccggccgg ggcgcagccg attagctcag atctgcagca gcacccgttc
      601 gatgacgccg aggatcagga aaacgaggat cggcgagaag tcgagaccgc ccatgggtgg
      661 caggagcttg cgggccggtg ccaggacggg ctctgtgagc tgctggagca gggccagcgc
      721 cgggtggtag ttacccggag cgaggaagct cgcgatgatc acgatcagaa tcgaccactt
      781 gatgatgaac agccccagca gaatcatctg cagcacccct gaggttgcga tctgcacagc
      841 ggttcccgtg ggccgatcgg cgatggcccc cagggcgagg atcatcacga cctccacgat
      901 gagcgctgcc aggagggcgg cgaaatccag atttcggtat ccgggcagca ccatccggac
      961 cggcttcaac accacgtcgg tggctttgac gatcccttga gaaatggggt tgtagaagtc
     1021 ggcccggcat gcctgcagca ggaaacgaac cacgaacagg aaggcaatga cctgcagaat
     1081 gaagctggtt gcgtaatata agacctctgc catgctcgtt gcctattccc ctgattacga
     1141 cgtttagcga ccgaactcga tggcgagctc gcgggatcgt tccgcggcgg ccgctacagc
     1201 ggcgttgatg ataccccggc agtcggcggc atccagtatt tccagcgccg cctgggtcgt
     1261 cccgcccgga gaggtcacat ttgccctgag ctgcgcgggt gggggccctt cgagagccat
     1321 cagcgcggcg ccgcgggcgg tttccagcgt cagggtgacg gcggtctcgg cgctgagccc
     1381 cagggcctgg ccggcttcga tcatcgcttc catcaggtaa aagaagtagg cgggcccgct
     1441 gccggagacg gcggtcaccg cgtcgagctg atcttccaag gatacccaga gggtcctgcc
     1501 cgcggtggcc agcacgcttt ctgcgagctc cctttgagcc ggcgagacgc gctcgttggc
     1561 gcacagggcg gtgactccgg cgccgagaag ggccggcgtg ttcggcatgc agcggacgat
     1621 gggctgctcg ctgctggtcc aaccggcaag gctcgacatg ggaacgccgg cggcgatgga
     1681 gaccaggagc tgatccgtgc tcagcgccgt cagccccccg gctaccgagg ccatgacctg
     1741 cggcttgacc gcgaggatca ccacgtccgc accgcgtacc tgggcattgt tgtcgtcgga
     1801 ggttgccacg ccgagctcgg cgatcttgcc cagctgctcg ggtgcggggt cgctggcgcg
     1861 aatggtcgcc gcatcgtgac cgctggcgat caggcctttg atcaggctgg tcgccatgtt
     1921 gccggcgcca atgaaagtaa tgtgactgct caacggatcg gaactccggg tagtggctcg
     1981 ttcgggttag ccctcgcgcg cgccgaagat ggcggtgccg atgcgcacgt gggtggcgcc
     2041 ggccattatg gcctgctcgt agtcgttgct catccccatg gacagggtat cccagcgacc
     2101 ggcggcgagg ggcgcgagcc cggcgaacag cttcgccatg cgcgcaaagc tctccggggc
     2161 atccgcgttc gggtcgagga tggtcatgag gccgcgcacg cagatccgct cgagatcaac
     2221 gagctccttc agcagatcgg cgaccgcggc tgggtcgacg ccggccttgc cgggatcggc
     2281 gtctatgttg acctgcagac agacctgcag ggttttgtcg tcaggacact gctcgttgag
     2341 ccggcgggcg atcttgggtc gatccaccgt atgcacccag tcgaaatgcc gcgccacctg
     2401 gcgggtcttg ttcgactgca gcgcgccgat gaagtgccag gtcaggggca gatgcccgag
     2461 cgcggcgatc ttcggcagcg cttcctgcag atagttttcg ccgaagtcct tcaagccggc
     2521 gccgtgggcc tcttcgatgg cacgggccgg ttgccgcttg gagactgcga tcaggcagac
     2581 ctcgtcgggg tcccgctgtg cggctcgcgc cgcagagaca acacaggaac gcacgctggc
     2641 gacgttgtga gagatcgagt gcaatttcgt tttgccaact gcgtcgcaga tttcgcatgt
     2701 ctccggcaga gcgaggaggg attcatggtt aaagcccagg tcagataata ctatacagcc
     2761 cggattcgca gcggctttcg ttgccatttc ggcgagtttg aagcccaagc gagtagacaa
     2821 ggatcgctga tggacattac cgaactcctc gctttcagcg ccaagcaggg cgcatcggac
     2881 ctccacctgt ccgcggggct tccgccgatg attcgtgttg acggtgacgt tcgccggatc
     2941 aacctgccgc cgctcgagca ccagcaggtg cattcgctga tctacgagat catgaacgac
     3001 aagcagcgca aggacttcga agagttcctc gaaaccgact tctccgtgga agttcccggc
     3061 gttgcgagat tccgtgtcaa cgcgttcaac cagaatcgtg gcgcgggcgc ggtgttccgg
     3121 accatcccgt ccaaggtgct caccatggaa gacctggaca tggggcaggt tttccgcgac
     3181 atctgcctcg ttcccagggg cctggtgctc gtcacgggac ctacgggatc cggtaaaagc
     3241 acgtcgctcg ccgccatggt cgacttcatc aacgaccatc gctacgacca catcctcacc
     3301 atcgaagatc ccatcgaatt cgttcacgag agcaagaagt gcctggtgaa ccagcgcgac
     3361 gtgcatcgag atactcacgg gttcaacgaa gccctgcgct cggcgctgcg ggaggacccg
     3421 gacatcatcc tcgtcggcga gatgcgcgac ttggagacga tccggctagc gctcaccgcc
     3481 gccgaaacgg gccacctcgt gttcggcacg ctgcacacga cgtcggcggc caagaccatc
     3541 gaccgggtca tcgacgtgtt tcccgcagaa gagaagtcca tggttcggtc catgctctcg
     3601 gagtcgctgc agtcggtgat ctcgcaaacg ctcctcaagc gggtcaacgg cgggcgcgtg
     3661 gccgctcacg agatcatgat cggcacggcg gccatccgga acctgattcg cgaggacaag
     3721 gtcgcgcaga tgtactcggc catccagacc ggcagctccg taggcatgca gacgctggat
     3781 cagtgcctgc tcgatctcgt tcagaagcgc acgatcaccc gcgactcggc tcgcgagaaa
     3841 gccaagaacc cggatgactt ctaacggcgg aggttagcgc catgcaattc gaaaagctgt
     3901 tgaaactggt ggtggaaaaa ggcgcgtcgg accttttcat caccgccggc aagccgccca
     3961 tgctcaaggt caacgggaag ctgttcgccg ctggaaccac caacctgtct gccgatcagt
     4021 cccgggatgt cgtgcacagc atcatggacg aggaccagaa gaagcagttt cacgaccatc
     4081 acgagtgcaa cttcgccatt cattccgaag ggctgggtcg ttttcgggtg agcgcgttcc
     4141 agcagcgcaa cctggcaggc atggtgctgc ggcgcatcga ggtcaacat
//
LOCUS       DBNHOX010000053         3388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1740140, whole genome shotgun sequence.
ACCESSION   DBNHOX010000053 DBNHOX010000000
VERSION     DBNHOX010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1740140"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="AC16UZ_01145"
     CDS             complement(<1..402)
                     /locus_tag="AC16UZ_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245311.1"
                     /translation="MSDRAPLIVDRTGEPLTTSEPARSKPARDWPPITITREMIDEEI
                     ERLADLPQPANGRRQAMFVHPNAPDPGRGLTPGVQVTLDVLKPGEETVPVRHNSTQVS
                     FCIRGGGTAIVGDRPIRYAQYDAWNHPSYATY"
     gene            502..1734
                     /locus_tag="AC16UZ_01150"
     CDS             502..1734
                     /locus_tag="AC16UZ_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6245312.1"
                     /translation="MDVDGATWSWPDGGVTEVPYAIYTDPGIFAREMERIFSGPSWAY
                     VGLEAEIPQPGDYLVTRIGVRSVVIARDEEGAIHGFANRCAHRGVKVCRQRHGNARFF
                     VCPYHQWSYDARGRLRGVPFINGVRQQGGMPEGFAREDHNLPPLDIATRGGVIFASFS
                     ATAPRLEDYLGDTNLSYFDRVFDGRQLKVLGYSRQRIPGNWKLMFENIKDPYHASLLH
                     VFLVTFGLFRADQPAQVRMDPSGAHGLLISRRGEQGATADTGEMSSMKEDFSLADARL
                     LDPVKEFPDDATVVMQTIWPNLIVQQQSNTLALRQIQPLDPGRFELHWTFFGYGDDDD
                     AMTLRRLRQANLMSSAGLVSADDSEVIKLAQDGVAQYPDDVGVMLMGGRGTDDEDHMV
                     TETAIRAFYAHYRRVMGL"
     gene            1731..2210
                     /locus_tag="AC16UZ_01155"
     CDS             1731..2210
                     /locus_tag="AC16UZ_01155"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013062.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HIR6245313.1"
                     /translation="MTLAAEERAELEEVYARYADLLDDGPLADWPALFHEECLYLLIP
                     RENHDRGLPLAIMRCESRGMLEDRVRAVQETIMHEPRYLRHHITNVRGARTDDGGYSV
                     IANYSVIEVLQDELPRILSVGRYLDVLTRSPTGALMFREKRVVFDSVMVPNTIVYPL"
     gene            2220..>3388
                     /locus_tag="AC16UZ_01160"
     CDS             2220..>3388
                     /locus_tag="AC16UZ_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR6245314.1"
                     /translation="MTLSDRLLDLGFLEAETPRTPMHFSALMFFDTPSDPAPFVSGLR
                     EAWTGAVPVAPFDLVPVHDLKSGLFLPRLAPAERVDVEDHLHLVTLPAGSGEQALLEL
                     AQRLHAECLDRSRPLWDVYLITGFGERCFAMFVKIHHAIFDGHGGLRLFVETLEEAPV
                     TRLPEPFWSRPSAAAPGHGRPLGPFEAVGALARSAGGLFRNAAVGARHASRWLLGSAP
                     VAPPAFSEPASILNGPSSGRRRLAHLSLPLPEIERCAADHGASVNDVLLAICGGALRR
                     DLEARRALPDRSLTALVPCSLRADAGRLPAGNRLTFIPMPLGTDIAEHPDRVSAVRAG
                     AGEAKERLEELGDPGALQYGMLINAVATLPRLARLPLPPISPANLVVTNLAGPAAP"
BASE COUNT          510 a         1120 c         1137 g          621 t
ORIGIN      
        1 gtaggtggcg taggaggggt gattccaggc gtcgtattgc gcgtaccgga tgggccggtc
       61 gccgacgatg gccgtgccgc cgccccggat gcagaagctc acctgggtgg aattgtggcg
      121 gacgggaacc gtttcctcgc cgggtttcag aacgtccagg gtcacctgaa cgcccggggt
      181 gaggccccgg cctggatccg gcgcgttggg atggacgaac atcgcctggc gtcggccgtt
      241 ggccggttgc ggcagatctg ccagccgctc gatctcctcg tcgatcatct cccgggtgat
      301 ggtgatcggc ggccagtccc tggccggctt tgatcgggcc ggctccgagg tggtcagcgg
      361 ctctccggtg cgatcgacta tcagtggtgc gcggtcgctc attcttggcc tctcgagcgg
      421 gaagctggac cggcgaagcc ggcgcgttga aatactttga tatcaaagta atatgtggga
      481 cgtcaactgc ggggttagct catggatgtc gatggcgcga cgtggtcgtg gcccgatggt
      541 ggggtgaccg aggtgcccta cgccatctac acggaccctg ggatttttgc ccgggagatg
      601 gaacgcatat tctcgggtcc gagctgggcc tacgtcgggc tggaggcgga gatcccccag
      661 cccggggact atcttgttac acgaatcggc gttcggtccg tggtgatcgc cagggacgag
      721 gaaggtgcga tccacggctt cgccaatcgc tgcgcgcacc ggggagtgaa ggtttgccgc
      781 cagcgtcacg gcaacgcgcg attcttcgtc tgcccctacc atcagtggtc ctacgatgcc
      841 cggggccgcc tgcgcggcgt tcccttcatc aacggcgtcc ggcagcaggg aggaatgccc
      901 gaggggttcg cccgtgagga tcacaacctg ccgcccctcg acatcgccac ccgtggcggc
      961 gtcatcttcg cgtccttctc cgcgacggcg ccgcggctcg aggattacct cggtgacacc
     1021 aacctctcct acttcgaccg tgtgttcgac ggccggcagc tgaaagtcct cggctactcg
     1081 cggcagcgca tccccgggaa ctggaagctc atgttcgaga acatcaagga tccctaccat
     1141 gcgtcgctgc tgcatgtctt tctcgtgacc ttcgggctgt tccgggcgga tcagcctgcc
     1201 caggtgcgca tggaccctag cggcgcccat gggctgctga tctcgcggcg gggagagcag
     1261 ggagcaacgg cggacaccgg ggagatgtcc agcatgaagg aggacttctc cctggcagac
     1321 gcgcgtctcc tggatccggt caaggagttt ccggacgacg ccacggtggt gatgcagacc
     1381 atctggccga atctgatcgt gcagcagcaa tccaataccc tggcgctgcg ccagatacag
     1441 cctctggacc ccggccgctt cgagctgcac tggacgttct tcggctacgg ggacgacgac
     1501 gatgcgatga cgctgcggcg tctgaggcag gcgaacctga tgagctcggc cggtctggtc
     1561 tccgcggacg acagcgaggt gatcaagctg gctcaggacg gcgtcgccca gtatcccgac
     1621 gacgtcgggg tgatgctcat gggcggccga ggcactgacg acgaggacca catggtcacg
     1681 gaaaccgcca tcagagcgtt ctacgctcat taccgacggg tgatggggct gtgaccctcg
     1741 ccgccgagga gcgcgccgag ctggaggagg tctacgctcg ctacgccgac ctcctggatg
     1801 acggcccgct ggcggactgg cccgcactct tccacgagga gtgcctctac ctgcttatac
     1861 ccagagagaa tcacgatcga ggcctgccgc tcgccatcat gcgctgcgag agccgtggga
     1921 tgctggagga tcgggtccgt gcggttcagg aaaccatcat gcacgagccg cgctaccttc
     1981 ggcatcacat caccaacgtc cgcggtgctc gcaccgacga cggtggttac tcggtgattg
     2041 ccaactactc cgtcatcgag gtgctgcagg atgagttgcc gcgcattctg agcgtgggtc
     2101 ggtatctcga cgttttgaca agatccccaa cgggcgcgct gatgttccgg gagaagcggg
     2161 tggtattcga ttcggtgatg gtgcccaaca ccatcgtcta cccgctgtag gtcggcgcgg
     2221 tgaccctctc cgaccggctc ctcgatctcg gcttcctgga ggcggagacg cctcgcacgc
     2281 ccatgcactt cagcgcgctg atgttcttcg atacgccatc cgatccggcg cccttcgttt
     2341 ccgggttgag agaggcttgg acgggcgcgg ttccggtggc gcccttcgac ctggttcccg
     2401 tgcacgatct gaaaagcggg ctctttctgc cgcggctggc cccggccgag cgcgtggacg
     2461 tggaggatca tctgcatctg gtaacccttc cggccgggag tggcgagcag gcgctcttgg
     2521 aacttgccca gcgcctccat gccgagtgtc tcgaccggag ccgcccgctc tgggacgtgt
     2581 acctgatcac cggcttcggc gagcgctgtt tcgcgatgtt cgtgaagatt catcacgcga
     2641 tcttcgacgg gcacggcggc ctgcgcctgt tcgtggagac cctggaagaa gcccccgtga
     2701 cgcggctgcc ggagccgttc tggtccaggc ccagcgccgc ggcccccggc catggccgcc
     2761 ctctgggtcc gttcgaggcg gtgggtgccc tggcgcgttc ggccggcggg ctcttccgga
     2821 acgcggccgt tggcgcgcgc catgcctccc gctggctgct cggttcggcg ccggtggccc
     2881 cgcccgcatt cagcgagccg gcgagcatcc tcaacgggcc ctccagcggt cgacgccgac
     2941 tggcccacct gagcctgccg ctgcctgaga tcgagcgatg tgcggccgac cacggggcat
     3001 ccgtcaacga cgttctgctc gccatctgcg gcggcgcgct gcgccgcgat ctcgaagcgc
     3061 gccgcgccct gccggaccgc tcgctcaccg cgctggtgcc ctgctcgttg cgggccgacg
     3121 ccggccggct gccggccggg aaccggctca cgttcatacc catgccgctt ggcaccgaca
     3181 tcgccgaaca tcctgaccgc gtttcggcgg tccgtgccgg ggcgggcgaa gccaaggaaa
     3241 ggctcgagga actgggcgac cctggcgccc tgcagtacgg catgctcatc aatgccgtgg
     3301 cgaccctgcc gagactcgct cgcctcccgc tgccgcccat ctctcctgcc aacctcgtgg
     3361 tcacgaacct ggcgggccca gctgcgcc
//
LOCUS       DBNHOX010000054         6269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1097509, whole genome shotgun sequence.
ACCESSION   DBNHOX010000054 DBNHOX010000000
VERSION     DBNHOX010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1097509"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1097
                     /gene="trxB"
                     /locus_tag="AC16UZ_01165"
     CDS             159..1097
                     /gene="trxB"
                     /locus_tag="AC16UZ_01165"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR6245315.1"
                     /translation="MTATHHRLIVLGSGPAGYTATLYAARANLRPALITGVEVGGQLT
                     TTTEVENWPGGHAELQGPELMAQMAEHVERFEARVINDYITTADLEAKPIRLQGDSQY
                     TCDALIIATGASAMYLGLPSEEAFLGRGVSACATCDGFFYRGQAVAVVGGGNTAVEEA
                     LYLSNICSKVYLVHRRDELRAEKILQDRLFARENIEPVWNHTLAEVVGDDSGVTGVRL
                     DGTHGAEPMDLDVTGVFIAIGHRPNTEIFAGQLEMEGGYITTRSGLDGNATATSTPGV
                     FAAGDVADHVYRQAITSAGFGCMAALDAEKYLDESV"
     gene            complement(1113..1979)
                     /locus_tag="AC16UZ_01170"
     CDS             complement(1113..1979)
                     /locus_tag="AC16UZ_01170"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF016032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HIR6245316.1"
                     /translation="MPSTCTARACGELHNASLDVSIDTGYPPVINSRREAELAFRAAR
                     EVVGDVGLMQLDHPSMGAEDFSYYLERIPGCVLLMQVASSDLGKDLGQSVARPAESCR
                     TLGMRLVGAGTHPFSTRLAAITPGPRYESMERTSGLLGHRQITFSTHVHLSVIDGDEA
                     LWLARQLAPYLPLMLALSASSPFWQGHDTRFAAFRPRILASRFGLDARVAVNENGDVM
                     TLREVARQTLDLIADTAARFGDTDHLSRLARAISAGLPSERQRRRFAATGSLRAVVDA
                     VIDELLRDPVVG"
     gene            complement(2037..4826)
                     /locus_tag="AC16UZ_01175"
     CDS             complement(2037..4826)
                     /locus_tag="AC16UZ_01175"
                     /inference="COORDINATES: protein motif:HMM:NF015644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HIR6245317.1"
                     /translation="MYVLLFVALLGCGAWLAHSGSRHHRKVQVWGGAAIVGSAFGLML
                     LMAFWAEMLWFDSLGYQDRFWTFVWVRLLVPLLGGAAAGLTGLVLTRGAGIYLRRGVA
                     FACAAAGVIWGLASWQPVLLFLNRMETGIVDPLLGLDTGFYLFVLPLLDGVFWLGLFV
                     ALISLITVLALREQDGVVMPGPAFAVPKGRLLIPGASLTLTVVLAFGQLLSVLHLLYS
                     KLGVVMGPGWTDVHVRLPALILMGAIFLVAGMLPLFEGFRGFMARLVARWEAIAPVNV
                     VAVVGAWAVIGGAWLLLVVLLPALTQWLVVKPNEITFEKPYIENNIEFTRRGFKLDRV
                     EERQFSADAALGPESLADNRHLISEVRLWDWRALDAVYKQFQEIRLYYEFVDVDIDRY
                     RLGDRYRQVMVAARELAQRNLPEQSRTFVNQRFKYTHGYGYTLAPVSDFTPEGLPNLL
                     VKDIPPVTDQPELEVTRPEIYYGELTIEPVVTNTLEPEFDYPSGDANIYTRYSGTGGV
                     VLSSFWRTFLFGWKFDGTVFLLSSYPTPESRVLFYRQVPERVQRLAPFLVFDEDPYIV
                     AVDGRLKWIVDAYTSSSYYPYSQAFDSEEFFGLSRDEGQARVRTVQRLDGANYVRNSV
                     KAVIDAYDGSVDFYVFDEEDLIIQVWQRALPGLFKPRSEMPASLVAHVRYPQDFLLAQ
                     GLIFTRYHMSDPDVFYNQEDVWVRATEKHYHDITPVEPYYVMWELPGSDEAEFVLILP
                     FTPKNRQVLIGWVAALSDGENYGRFIAYKFPKERRVLGPQQVETKIDQDSFLSGQLTL
                     WDQQGSEVIRGNVLAIPIDDSLLYVEPIYLRAETAAYPELRLVAVMQGDNLSYAETLD
                     EALLGLFTGRQLVGETPSRSVKGLAIEARMAFESYLEALGDKRFEAAGEALRRLSQLL
                     ERIDAAASVGEGNGP"
     gene            complement(4932..5354)
                     /locus_tag="AC16UZ_01180"
     CDS             complement(4932..5354)
                     /locus_tag="AC16UZ_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013319.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR6245318.1"
                     /translation="MSDDRAIASWSHFDHAADMGVRGVFARCFPAARLEMLFDVSHNT
                     CKVETHRVGDETRELFVHRKGVTRAFGPGHPSLPDPLRSVGQPVLIGGSMATESYVLR
                     GTKESERRAYSSACHGAGRAMSRRAATRRWRGWFPCSA"
     gene            complement(5481..6266)
                     /locus_tag="AC16UZ_01185"
     CDS             complement(5481..6266)
                     /locus_tag="AC16UZ_01185"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6245319.1"
                     /translation="MYNRTGAKSERWVTEHGGSRVETPREAASGADVVCLCVGNDDDV
                     RAVVYGPEGVLDGLADGSVLIDHTTVSAELAEELASAAGERGCGFLDAPVSGGQAGAE
                     NGQLTVMVGGEQTIFDSALPVMEAYGKSVRRMGPAGTGQLTKMVNQICIAGVLQGLAE
                     GLHFADQAGLDIESVVDVISKGAAQSWQMENRHATMARGEFDFGFAVDWMRKDLAIAL
                     QAGRRLQARLPLTALVDQFYADVQSYGGGRWDTSSLIQRLRRT"
BASE COUNT         1216 a         2107 c         1962 g          984 t
ORIGIN      
        1 cgtcgcggtc gatgcgatcc tgaatatcca agctggcccc gcccgcgaat caataggccc
       61 aataaacctc caataccgag ccggagtcaa cgcgtgagcg ttcacccatg ggcgtgcgct
      121 gtataaaatg cccgctcgct gatccacagg acacaaccgt gaccgccaca catcatcgac
      181 tcatcgtgct cggatcaggg ccggccggat acaccgcaac gctgtacgcg gcccgggcga
      241 acctgcgacc cgcgctgatt accggggtgg aggtcggcgg gcagctgacc acgacgaccg
      301 aggtggaaaa ctggcccggc gggcacgctg agctccaggg ccccgagctg atggcccaga
      361 tggctgaaca cgtggaacgc ttcgaggcga gggtcatcaa cgactacatc accaccgcag
      421 acctcgaggc caagcccatt cgcctccagg gagactccca gtacacgtgc gatgccctga
      481 tcatcgcgac cggcgcgtca gccatgtatc tcgggctgcc ttcggaggag gcctttctgg
      541 gtcgcggcgt gagcgcctgc gcaacatgcg acggtttctt ctatcgcggc caggccgtcg
      601 ccgtggtcgg cggaggcaac acggccgtgg aagaggcgct gtacctgtcg aacatctgca
      661 gcaaggtcta cctcgttcac cgccgcgacg agctgcgcgc ggagaagatc cttcaggacc
      721 gcctgtttgc ccgggagaac atcgagccgg tatggaacca caccctggcc gaggtggtcg
      781 gtgacgattc cggcgtcacc ggggtgcgcc tggacggcac ccatggtgcc gagccgatgg
      841 atctggacgt gacgggcgtg ttcatcgcca tcggccaccg gccgaacacc gagatcttcg
      901 ccggccagct cgagatggag ggcggctaca tcaccacccg cagcggtctg gacggcaacg
      961 ccaccgcgac cagcaccccg ggggtattcg cagccgggga cgtggccgat cacgtctacc
     1021 gccaggcgat tacctccgcc gggttcggct gcatggccgc gctggacgcc gaaaaatatc
     1081 tggacgagtc ggtctgagcg gcaggcccgg tatcatccca ccacggggtc ccgcagaagc
     1141 tcgtcgatga cggcatccac caccgcccgc aacgagccgg tcgccgcaaa cctacgccgc
     1201 tggcgttccg atgggagccc tgcggagatc gctcgtgcca gcctggaaag gtggtccgtg
     1261 tctccgaacc gcgcagccgt gtcggctatc agatcgagcg tctgacgtgc gacctcgcgc
     1321 agcgtcatca catcgccgtt ctcgttgacg gccacccggg cgtcgaggcc gaaacgagag
     1381 gccaggatcc gcggccggaa agcggcaaaa cgggtgtcgt gaccctgcca gaatggcgag
     1441 ctcgccgaga gcgccagcat cagcggcagg taaggggcca gctgcctggc gagccatagg
     1501 gcctcgtccc catcgatgac gctcaggtga acgtgggtag aaaaggtgat ctgacgatgc
     1561 ccgagcagcc ccgacgtccg ctccatggat tcgtatcggg ggcctggcgt gatggccgca
     1621 agcctggtgg agaacggatg cgtccccgcg ccgaccagcc gcattcccaa cgtccggcag
     1681 ctttcggcag gtcgggcgac gctttggccg aggtccttgc cgagatcgct cgacgcgacc
     1741 tgcatgagga gaacgcagcc gggaatccgt tccaggtagt aggagaaatc ctcagcaccc
     1801 atgctcgggt gatcgagctg catcaggccg acgtcgccaa ccacttcgcg agcggcccga
     1861 aaggcaagct cggcctcccg gcggctgttg atcaccggcg ggtagcccgt gtcgatggat
     1921 acgtccaggg acgcgttgtg cagctcgccg cacgcccggg ccgtgcacgt cgatggcaaa
     1981 tcggtccgat tgggccgtga tcgtaccggc gctcaccgcc gaatcgaccc attgcgtcac
     2041 ggcccgtttc cttccccaac cgacgctgcc gcatcgatcc gctcgagcag ctggctgagc
     2101 cggcgcaacg cctcgcccgc cgcttcgaag cgcttgtctc ccagcgcctc gagatagctt
     2161 tcgaaagcca ttcgcgcctc gatggccaac cctttgacgg accgagacgg cgtctccccg
     2221 accagttgcc ggcccgtgaa gaggcccagc agcgcctcat ccagggtctc ggcgtagctc
     2281 agattgtctc cctgcatcac ggcaaccagt cgcagctcag gatacgcggc ggtctcggcg
     2341 cgaagatata tgggctcgac gtacaggagc gagtcgtcga tgggtattgc caggacgttc
     2401 ccgcgtatca cctcggagcc ctgctggtcc cagagcgtca gctgccccga caggaacgag
     2461 tcctgatcga tcttggtttc gacctgctga ggcccgagca cccgccgttc cttgggaaac
     2521 ttgtaagcga tgaagcgccc gtagttctcg ccatcggata gcgcggcaac ccagcctatg
     2581 agcacctgcc ggtttttggg cgtgaacggc aggatgagca cgaattccgc ttcgtccgag
     2641 cctggcagct cccacatcac gtaatagggc tccaccggcg tgatgtcgtg atagtgcttc
     2701 tcggtggctc gcacccaaac gtcttcctgg ttgtagaaga cgtcgggatc ggacatgtgg
     2761 tagcgggtga agatgagccc ctgagcgagc aggaagtcct ggggataacg cacgtgggcg
     2821 accaggctgg ccggcatttc ggagcggggc ttgaacaggc ccggcagcgc gcgctgccac
     2881 acctgaatga tgaggtcttc ttcgtcgaac acgtagaagt ccacgctgcc gtcgtaggcg
     2941 tcgatgacgg ccttgacaga gttacgcacg tagttggcgc cgtcgagccg ctgcaccgtc
     3001 cgcacccgcg cctgaccctc gtcccgactc agcccgaaga actcttcgct gtcgaaggcc
     3061 tggctgtaag ggtagtagct cgaactcgtg taggcgtcca cgatccactt cagacgcccg
     3121 tccacggcaa cgatgtaggg gtcctcgtcg aagaccagaa acggggcgag ccgttgcacc
     3181 cgctccggca cctgacggta aaacaagacc cggctctccg gcgtggggta actcgacaac
     3241 aggaaaaccg tgccgtcgaa cttccagccg aacaggaacg tgcgccagaa gctcgatagc
     3301 accacgcccc cagtacccga gtagcgggtg tagatgttgg catccccgct tggataatcg
     3361 aactcgggct cgagggtatt ggtgaccacc ggctctatgg tgagctcgcc gtagtagatc
     3421 tccggccggg ttacctccag ctccggctgg tcggtgaccg gcggaatgtc cttgaccagc
     3481 aggttgggca acccttccgg cgtgaaatcg ctcaccgggg ccagggtgta accgtaaccg
     3541 tgggtgtact tgaagcgctg gttgacgaac gtgcggctct gttcagggag gttacgttga
     3601 gccagttccc gtgccgccac catgacctga cgatagcggt cacccagccg gtagcggtcg
     3661 atgtcgacat ctacgaactc gtagtaaagg cgtatttcct gaaactgctt gtagacggcg
     3721 tcgagagcac gccaatccca cagccgtacc tccgaaatga gatggcggtt gtcggccaga
     3781 gactccgggc cgagcgccgc gtcagcggaa aactgccgct cctccacccg atcgagcttg
     3841 aaaccacgcc gtgtgaattc gatgttgttc tcgatgtagg gcttctcgaa ggtgatctcg
     3901 ttgggcttga ccaccagcca ctgggtcagc gcagggagca gaaccaccag cagcagccat
     3961 gcgccaccga tcaccgccca cgcaccgacc acggcgacca cgttgacggg cgcgatggcc
     4021 tcccagcgcg cgaccagccg cgccatgaag ccacgaaaac cctcgaagag cggcagcatg
     4081 ccggccacca ggaaaatggc gcccatcaga atcagggcag gcagccgaac gtgaacgtcg
     4141 gtccagcccg ggcccatcac cacgccgagc ttcgagtaca gcagatgcag aacgctcaag
     4201 agctggccaa aggccagcac gacggtcagc gtcaggctgg cgccgggaat gaggagccga
     4261 cccttcggca ccgcgaacgc cggccccggc atcaccaccc cgtcctgctc gcgcaacgcc
     4321 aggaccgtga tgagcgagat cagagcgacg aagaggccca gccagaagac gccgtcaagc
     4381 aggggcagaa cgaaaaggta gaaccccgtg tccagcccga gcagcgggtc gacgatcccg
     4441 gtttccattc gattgagaaa gagcagcacg ggctgccagg atgcaagccc ccagatcact
     4501 ccggccgcgg cgcaagcgaa ggccacgccg cgtctgaggt agatgcccgc gccccgcgtc
     4561 agcaccagtc ctgtgaggcc cgccgcggcg ccgcccagca gaggcaccaa aaggcgaacc
     4621 cagacgaagg tccagaaccg atcctgatag ccgagcgagt cgaaccacag catctcggcc
     4681 cagaaagcca tcagtaacat cagcccgaac gcggaaccga cgatggctgc accgccccag
     4741 acctgcactt tgcggtgatg ccgactgccg gaatgagcca gccaggctcc acagcccagc
     4801 agagcaacga aaagcaggac gtacatggtt cacacccttc ttgccgaccg ttccagtctg
     4861 gcaggcgggt tcgcctgggc aaccgtggtt actacctaga ccttaaggcc gtgtcgggcg
     4921 ccgttagccc ttcacgcaga gcacgggaac cagccgcgcc agcgccgggt ggccgcgcgg
     4981 cggctcatgg cccgacccgc cccatgacag gcggaggagt aggcgcggcg ctcgctttcc
     5041 ttcgtcccgc gcagaacgta agactcggtt gccatgctgc cgccgatcag caccggctgc
     5101 cccacggacc tcagggggtc gggcaggctc gggtgaccgg ggccaaacgc tcgcgtgaca
     5161 cccttgcggt gaacgaacag ctcccgggtc tcgtcgccaa cccggtgggt ttccaccttg
     5221 cacgtgttgt gagagacgtc gaaaagcatc tcgagccgcg ctgccgggaa gcatctggcg
     5281 aacacgccgc gaacccccat gtcggccgca tgatcgaagt ggctccaact cgcgatggcc
     5341 cgatcgtcgc tcaactgtca gccccatgat tcgcacggtt gccgcgcgca acaaatgccg
     5401 tgatcggcga cgatttccga cgctaccgcg tagccgaaag ctcgaacata gggtatgtcc
     5461 gaggtggcga tccaaggtgg tcaggtgcgc cgcaaacgct ggatcaggct ggaagtatcc
     5521 caacggccgc caccgtagct ctgcacgtcg gcataaaact ggtccacgag tgccgtgagc
     5581 ggcaaacggg cctgcagccg acgtccggcc tgaagcgcga tggcgagatc cttgcgcatc
     5641 cagtcaacgg cgaaaccgaa atcgaactcg ccccgggcca tggtggcgtg ccggttctcc
     5701 atctgccagg actgggcggc gcccttggag atcacgtcca ccaccgattc gatgtccagc
     5761 ccggcctgat cggcaaagtg cagcccctcc gccagcccct gcagaacccc ggcaatgcag
     5821 atctggttga ccatcttggt gagctgcccg gtgccagcgg gacccatgcg acggaccgat
     5881 ttgccgtagg cttccatgac gggcagagcg ctgtcgaaga tcgtctgctc cccgcccacc
     5941 atcaccgtca gctgtccgtt ctcggctccc gcctgacctc cggagacggg cgcgtccaga
     6001 aacccgcaac cgcgctcgcc ggcggcagaa gccagctcct cggccagctc cgcggagacc
     6061 gtggtgtggt cgatcagcac cgagccgtcg gccagcccgt cgagcacacc ctcgggcccg
     6121 tagacgacgg cgcgcacgtc gtcgtcgttg cccacacaaa ggcagaccac gtccgccccc
     6181 gacgcagcct cccgcggggt ttcgacccgg gacccgccat gctcggttac ccatcgctcg
     6241 gacttggcgc cggttcggtt gtacacgcg
//
LOCUS       DBNHOX010000055         4350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_519039, whole genome shotgun sequence.
ACCESSION   DBNHOX010000055 DBNHOX010000000
VERSION     DBNHOX010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_519039"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="AC16UZ_01190"
     CDS             complement(<1..1102)
                     /locus_tag="AC16UZ_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245320.1"
                     /translation="MLNVETLRVTLVAAMVLLAAQPAHADGTYGADFRAHLLPEAGTA
                     HASITITQEAGELKRLDLAAPAATYSAFSGPGSIRREEDRLVWTVPEDGGTLSYDVLI
                     DHRRNGAYDARMTPDWAIARLDDLFPRARTRSESGAQSRSSVVLAGPAGWRFETRFGP
                     SHEQVAVVDEGRRFDRPTGWLVAGRLGIRRDTIAGRTVSVAAPVGQGFRRVDVLAFLR
                     WTLPDLVAVFPGMPDRLLVVGAPEGMWRGGLSGPESLYLHVGRPLISENGTSTLLHEL
                     VHIATTAPKSAGDDWIMEGLAEYYSLETLRRSGGISEERFASALGSLAAWAQRDGGRL
                     ADPSTGAHTARAALTFHELAAELEDAGSSLDAV"
     gene            1264..2031
                     /locus_tag="AC16UZ_01195"
     CDS             1264..2031
                     /locus_tag="AC16UZ_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6245321.1"
                     /translation="MIDVYYWPTPNGWKVTIMLEECGLPYRILPVDIGGGDQFTPDFL
                     RVSPNNRMPAIVDHEPLGGGEPLSIFESGAILEYLADKSGKFLSREPAGRYRTLQWVH
                     WQMANLGPMMGNANHFKNYGKNIAEDPQQLEYGTRRFVGEVDRLSGVMDAQLRVNRYL
                     AGSEYTIADIITWPWAMLLGRLIDEAVWETFPNLKRWVDEVGERPGVEKGRKVERDLG
                     RQERTEEQEKARRELLFNQTNEKVRVAREAAAKAAAG"
     gene            2159..2539
                     /locus_tag="AC16UZ_01200"
     CDS             2159..2539
                     /locus_tag="AC16UZ_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6245322.1"
                     /translation="MAIRELVDSYNDAVMRFDGDAWAANWRDDATWSLPGAGPIKGKE
                     NFFPMWQQAMSAFSFVGFFASAGPIVVDGDTAHATWYQQEFLHQKDGVKRNVTGRYED
                     DYVKVDGRWYFQQRIYEVMNAEES"
     gene            complement(2545..3705)
                     /locus_tag="AC16UZ_01205"
     CDS             complement(2545..3705)
                     /locus_tag="AC16UZ_01205"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6245323.1"
                     /translation="MAESKRDTSADYGPHEEAMGAYLREGEARALALGNRGPIRFGAD
                     GRLAADILDAYSRLGFYVFTGVLSAEELDDLRSDVEDMWSRLPVSRGAAVDALGRPAL
                     GADLRAPTVQWARPLSDPLGGTDKANGRHPAKMFEPQPPADAPPFVCQLVLGSLQFSD
                     ACLRVYGHPQLLAVAAAINGDDFAPFNEAVWRKEPGLGPSVAWHQDGTTHWDSPDLDE
                     GPPGFNFMAQIYGCTAANAVWVVPGSHARGKVDIAALVARAGSDRLPDAVPLICDPGD
                     VAICNRQAVHGSFANTGGDLRVTVNFGFHRRQSLLGVEGGGIHNRREIYDERRIRERS
                     RVIGYAIDARRQRFPDEAPYVYRPFAGEEDEYRWSDAARARLKDYNLLDLGI"
     gene            complement(3795..>4350)
                     /locus_tag="AC16UZ_01210"
     CDS             complement(3795..>4350)
                     /locus_tag="AC16UZ_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclohexanone monooxygenase"
                     /protein_id="HIR6245324.1"
                     /translation="SVTPIDTVTPKGLRVRNVDYEFDVLVLATGFDAMTGALLGMDIR
                     GVGGERLADHWRAGPRSYLGLCVTGFPNLVTITGPGSPSVLSNMMVSIEQHVDWVADC
                     LGHMADRGLTRLEALQQAEDGWVDHVNEVANATLFPKGGSWYLGANIPGKPRVFMPYA
                     GGVGPYRDICDGVAARGYEGFSIT"
BASE COUNT          792 a         1466 c         1428 g          664 t
ORIGIN      
        1 caacggcgtc gaggctgctg cccgcatcct cgagctccgc agcgagctca tgaaacgtca
       61 atgccgcgcg ggccgtgtgc gcacccgtgg atgggtccgc caggcgcccg ccgtcacgtt
      121 gcgcccaggc cgccagtgac cccagggccg acgcgaagcg ctcctcgctg atacccccgc
      181 tgcgccgaag ggtctccagg ctgtagtact cggcaagccc ttccatgatc cagtcgtcgc
      241 cagccgattt cggcgccgtc gtggcgatgt gcacgagctc atgaagcagc gtgctggtgc
      301 cgttctcgct gatgagcggg cgcccgacgt ggaggtacag cgattccggg ccggaaaggc
      361 cccctcgcca catgccctcc ggggcaccta cgacgagcag ccggtcgggc attccgggaa
      421 acacggcgac gagatcgggc agcgtccagc gcaggaaggc gagcacgtcc acccggcgaa
      481 agccctgccc caccggcgcc gccactgaaa ccgtccggcc ggcgatggta tccctgcgga
      541 tgccgagcct gccggccacc agccagccgg tgggccggtc gaaccggcgc ccctcgtcca
      601 ccacggccac ctgctcgtgg ctcggcccga agcgggtctc gaaccgccag cccgccggtc
      661 cggcaagaac gacgctcgac cgggattgcg cccccgactc gctgcgcgtc cgggcgcgcg
      721 gaaagagatc gtccagccgc gcgatggccc aatccggcgt catccgcgca tcgtaggccc
      781 cgttgcggcg atggtcgatc aacacgtcgt agctcagggt gccgccgtcc tcaggaacgg
      841 tccagaccag acgatcttcc tcgcgccgga tgctcccggg acctgaaaag gccgaatagg
      901 tcgccgccgg ggccgccagg tccaggcgct tgagctcgcc cgcctcctga gtaatggtga
      961 tcgaggcatg cgccgttccc gcttcgggaa gcaggtgcgc cctgaaatca gcgccgtaag
     1021 tcccgtcggc gtgagcgggt tgagcggcga gcagcaccat cgccgccacc agggtgaccc
     1081 tcagggtctc aacgttcagc atcgtttgac gactccacac ggcaccttcg cgataacggc
     1141 tggatgacac agcgcagata ccagagcgcc gtcgggattg gaagcgcgcc gacgtttgaa
     1201 tcgagaaatt gaatctccca tagtgtacgc gcggacgcga ctggcgctga gaggagcgaa
     1261 gcgatgatcg acgtgtatta ctggcctacc ccgaacggct ggaaggtcac catcatgctg
     1321 gaggagtgcg gcctgcccta ccggatactg ccggtcgaca tcggcggcgg cgaccagttc
     1381 acgccggact ttctgcgcgt gagccccaac aaccgcatgc cggccatcgt cgatcacgag
     1441 cctctcggcg gcggggagcc gctgtcgatc ttcgaatccg gcgccatact cgagtatctc
     1501 gccgacaagt cggggaagtt tctgtcccgg gagccggccg gccgataccg caccctgcag
     1561 tgggttcact ggcagatggc caatctgggc ccgatgatgg gcaacgccaa tcacttcaag
     1621 aactacggca agaacatcgc cgaggacccg cagcagctgg aatacggcac ccgtcgtttc
     1681 gtgggcgaag tggaccgtct gtccggcgtg atggacgctc agctccgcgt caaccggtat
     1741 ctcgccggca gcgagtacac catcgcggac atcatcacct ggccgtgggc catgctgctc
     1801 ggccgcctca tcgacgaggc cgtctgggag acctttccga acctcaagcg ctgggtggac
     1861 gaggtgggcg aacgccccgg cgtggaaaag ggccgcaaag tggaacgcga tctcggccgc
     1921 caggagcgga ccgaggaaca ggagaaagcc cggcgggagc tcctgttcaa ccagaccaac
     1981 gaaaaagtgc gtgtggcacg agaggccgcg gcgaaagcgg ccgccggctg acgggccgga
     2041 cctcgccacg cgctcgcaat gcgcagcgcg tcgtgattaa gtgtgattct tcgagaccgc
     2101 attcaagacc aagaagggag ggaagcaaat gatcagcagt ggaccactgg aagaccgcat
     2161 ggcaattcgc gagctagtcg actcgtacaa cgatgccgtc atgcgtttcg atggagatgc
     2221 gtgggcggcc aactggcgcg atgacgccac ctggtctctg cccggggccg gaccgatcaa
     2281 gggaaaagag aacttctttc ccatgtggca gcaggccatg agcgcgttct ccttcgtcgg
     2341 cttcttcgcc tcggccgggc ctatcgtggt ggatggcgac accgctcacg ccacctggta
     2401 ccagcaggag tttctccacc agaaggacgg cgtgaaacgc aacgtcacgg gacgttacga
     2461 ggacgactac gtcaaggtag atggccgctg gtacttccag cagcgcatct acgaggtcat
     2521 gaacgccgag gagtcgtaag cgccttagat ccccaggtcg aggaggttgt agtccttgag
     2581 ccgcgcgcgg gccgcgtcgc tccagcggta ctcatcctcc tcgcctgcga acggtcgata
     2641 gacgtagggc gcctcgtcgg gaaagcgctg cctgcgcgca tcgatggcgt aaccgatcac
     2701 acgcgaccgc tcgcggatac gccgttcatc gtatatctcc cggcggttgt ggatgccgcc
     2761 gccctccacg ccaagcagcg attggcgtcg gtgaaatccg aagttgacgg tgaccctcag
     2821 atcgcccccg gtattggcga aggatccgtg taccgcctga cggttgcaga tggccacatc
     2881 tccgggatcg cagatgaggg gcaccgcgtc gggaaggcga tccgagcctg cgcgggcgac
     2941 gagcgcggcg atgtcaactt ttccgcgagc gtgtgatccc ggcacgaccc agacggcgtt
     3001 cgccgccgtg cacccgtaaa tctgcgccat gaaattgaat ccggggggtc cctcgtcgag
     3061 atcgggactg tcccagtggg tggtaccgtc ctggtgccag gcgaccgacg ggcccagacc
     3121 cggctccttc cgccagacgg cttcgttgaa cggcgcgaaa tcgtccccgt tgatggctgc
     3181 cgccaccgcg agcagctgag gatggccgta aaccctgagg cacgcatcgg agaactgcag
     3241 ggacccgagc accagctggc agacgaaagg cggcgcatcg gccggcggct gcggctcgaa
     3301 catcttggcc gggtgccgac cgttggcttt gtccgtgccg cccaggggat cgctgagggg
     3361 ccgcgcccac tgcaccgtgg gtgcgcgaag gtcggcgccc agcgcgggac gacccagcgc
     3421 gtctaccgca gcaccccggg agaccggcag tcgcgaccac atgtcctcga cgtccgaacg
     3481 gagatcgtcc agctcctcgg ccgacaacac cccggtgaac acgtagaagc ccaggcgcga
     3541 gtaggcgtcg aggatgtccg ccgcgagccg accatcggcg ccgaagcgga tggggccccg
     3601 gttacccagg gcaagggcgc gcgcctcgcc ttcgcgaaga taggcgccca tggcctcctc
     3661 gtggggcccg tagtcggcgc tggtgtcccg cttcgactca gccataccta tctcccgatc
     3721 gatccgcatc gcggtgcgcg cccccgaagt atcgcctcgg gcaagccttg ccgtcgaccc
     3781 gcgctgcggc gccgctaggt gatcgaaaag ccctcgtagc cgcgcgcggc cacgccatcg
     3841 cagatatcac gatacgggcc cacgccgccc gcatagggca tgaacacgcg cggcttgccg
     3901 ggaatgtttg cgccgagata ccaggatcct cccttgggga acagcgtggc gttggcgacc
     3961 tcgttgacgt gatccaccca accgtcctca gcctgctgca gagcctccag gcgggtgagc
     4021 ccgcgatcgg ccatgtggcc caggcagtcg gccacccagt ccacgtgctg ctcgatggaa
     4081 accatcatgt tgctcagaac agaggggctg cccgggccgg tgatggtcac caggttggga
     4141 aacccggtga cgcacaggcc caggtagctc cgcgggcccg cacgccagtg atcggccagc
     4201 cgctcccccc cgacaccccg gatgtccatg ccgagcagcg cgccggtcat ggcgtcgaag
     4261 cccgtggcga gcaccagaac gtcgaactcg tagtcgacgt tgcgcacccg caaccctttc
     4321 ggcgtgacgg tatcgatggg ggtaacggag
//
LOCUS       DBNHOX010000056         2360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1225768, whole genome shotgun sequence.
ACCESSION   DBNHOX010000056 DBNHOX010000000
VERSION     DBNHOX010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1225768"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="AC16UZ_01215"
     CDS             complement(<1..828)
                     /locus_tag="AC16UZ_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6245325.1"
                     /translation="MSSRAFARWNVAAAVLAASILGPMAAFSQQPPAPAVVVAPAAVV
                     DLRSSTSFTGRLVAIQKVDIRARVTGFLERVNFMEGKPVKAGDVLYRIERGAYAAAVA
                     ETQGAIRSAEAEQRLAEIERDRKAQLVARQTVAQSELDVAEAQLGHAQGEVERLMGTL
                     ARAELDLSYTEIRAPFNGIASLSAVDVGALVGPDTGPLTRLTRLDEVQAEFPIATAIL
                     LDHRERVKRGEASDEKTVSLTLPNGTVYPLKGDINFVDASVSRGTDTVTVRASFANPD
                     "
     gene            complement(1054..2180)
                     /locus_tag="AC16UZ_01220"
     CDS             complement(join(1054..1916,1916..2180))
                     /locus_tag="AC16UZ_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611339.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIR6245326.1"
                     /translation="MPGKRPKPEEIVSKLRQVEVLQGQGMPITDAVRQIGVTQQTYYR
                     WRKLYGGMGRAQLKRLKELEKENQRLRRAVSDLTLDKLILTEAAPGKLLSPSRRRRCI
                     DHVRRVHGVSERRACRALGQHRSTQRKIPQGRPDEERLTEDMIELARNYGRYGYRMIT
                     GMLNQSGWHVNHKRVERIWKREGLKVPRKQPKKGRLWLNDGSCVRLRPERPNHVWSYD
                     FVQDRTHDGRVYRTLNIIDEFTKEALMIRVQRKLNSTDVVDALTDLFILRGPPEFIRS
                     DNGPEFIAEKVRNWIAAVGAKTAFIEPGSPWENGYCESFNARFRDELLNGEIFYSLRE
                     AQILIEQWRRHYNTVRPHSALGYRPPAPESIITMDHRPVMH"
BASE COUNT          395 a          753 c          729 g          483 t
ORIGIN      
        1 atctggattg gcaaaggagg cacgaaccgt caccgtgtcg gtgccccgcg agacgctggc
       61 atcgacgaag tttatgtcgc cctttagcgg atagaccgtt ccgttaggca gggtcaggct
      121 caccgtcttc tcgtccgaag cctcaccgcg cttcacccgc tcgcggtggt cgagaaggat
      181 tgctgtcgcg atcgggaact cggcctgcac ctcgtcgagc cgcgtcagcc gggtgagcgg
      241 gcccgtatcc gggcccacca gtgcgccgac atcgaccgcg gagagactgg caatcccgtt
      301 gaatggggcg cggatctcgg tataggagag gtcgagctcg gcgcgcgcca gcgtacccat
      361 gaggcgctcg acttcgccct gggcgtggcc gagctgcgcc tcggctacgt cgagttctga
      421 ctgggccacc gtctgcctgg cgacgagctg cgccttacgg tcgcgctcga tctccgccag
      481 gcgctgttcc gcttccgccg aacggatggc gccctgggtc tcggcgaccg ccgccgcata
      541 ggcgccccgc tcgatccggt acagcacatc gccggctttg accggcttgc cttccatgaa
      601 gttcactctt tcaaggaagc ctgtgaccct ggcccggata tcgaccttct ggattgccac
      661 cagccggcca gtgaagctgg tgctcgaacg aagatccacg accgctgcgg gcgcaaccac
      721 tacggccggt gcgggaggtt gttgcgaaaa ggcagccatg gggcccagga ttgatgctgc
      781 caaaacggca gctgccacgt tccatcgcgc gaaagctctg ctcgacattc ccgatctctc
      841 ctgaggaaat agcgagcaga tgctcgcgta aggtgcccgt aaaatcaacc tcgatcacct
      901 ttcggctcgt ttcgagcccc atgaagtgaa gcgattggat gccatgatga ggatcggtca
      961 cgagcaaatg ccctcttctc gcgccgggca tttggccggg gccggtcaat gggtgacact
     1021 ggagagcccc cattgggtgg tccaacgtga ttgttagtgc atgaccggcc tgtggtccat
     1081 tgtgatgatg ctttctggcg ccggtgggcg gtatccgagg gcgctgtgtg ggcggactgt
     1141 attgtagtgg cggcgccatt gttcgatcag gatctgggct tcgcgcagcg agtagaagat
     1201 ctccccgttc agaagttcgt cccggaacct tgcgttgaag ctctcgcagt agccgttctc
     1261 ccagggtgag ccgggttcga tgaacgctgt cttggcgccg acggccgcga tccagttcct
     1321 gaccttctca gcaatgaact cggggccgtt gtccgagcgt atgaactcgg gcgggccgcg
     1381 caagatgaag agatcggtca gagcgtcgac aacgtcggtg gagttgagct tgcgctggac
     1441 gcggatcatc agcgcctcct tggtgaactc gtcgatgatg ttgagcgtcc ggtagacgcg
     1501 cccgtcatgg gtccggtcct gcacgaagtc gtaggaccac acatggttgg ggcgctccgg
     1561 ccggagccgg acgcaggagc cgtcattgag ccagaggcgg cccttctttg gctgtttccg
     1621 ggggactttc agcccttcgc gcttccagat ccgctcgacc cgcttgtggt tcacatgcca
     1681 gcccgactga ttgagcatgc cggtgatcat gcgatacccg taccgaccat aattgcgggc
     1741 cagctcgatc atgtcctcgg tcaggcgttc ctcgtctggg cggccctgcg ggatcttgcg
     1801 ctgggtcgag cgatgctgac caagagcgcg gcaggctcgg cgttcagaga caccatgaac
     1861 ccgccgcacg tggtcaatgc acctacgccg gcgcgaaggg ctcagaagtt tccctgtgcc
     1921 gcctccgtca ggatcagctt gtcgagcgtc aggtcggaca ccgcccgcct cagtcgctgg
     1981 ttctccttct caagctcctt gaggcgcttg agctgcgcgc ggcccatccc gccgtagagc
     2041 ttccgccaac ggtagtaggt ctgctgcgtc accccgatct ggcgcaccgc atccgtgatt
     2101 ggcattccct gtccctgcag cacctcaacc tggcgcagct tcgaaacgat ctcctcaggc
     2161 ttcggtcgct tccctggcat tctctggtcc tcctgaaaac gggctcatct gaaccctcca
     2221 agcggaccac ttcaatgggg gcattccacc tgcagcacct caacctggcg cagcttcgaa
     2281 acgatctcct caggcttcgg tcgcttccct ggcattctct ggtcctcctg aaaacgggct
     2341 catctgaacc ctccaagcgg
//
LOCUS       DBNHOX010000057         7699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_390296, whole genome shotgun sequence.
ACCESSION   DBNHOX010000057 DBNHOX010000000
VERSION     DBNHOX010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7699
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_390296"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="AC16UZ_01225"
     CDS             <1..493
                     /locus_tag="AC16UZ_01225"
                     /inference="COORDINATES: protein motif:HMM:NF052847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6245327.1"
                     /translation="GDLEIVVDFLSADAVQIEPGQRVIIERWGGESALVGHVRRIEPF
                     GFRKTSALGIEEQRVNVIVDFTSPRNEWSRLGHGYQVDVRVVLWEQDAVLAIPLTALF
                     RDDQRWAVFVDDDGRAALREVVVGHRNGMVAEIVSGLARGDRVVLHPSNRVIDGVRIT
                     GRG"
     gene            complement(528..2078)
                     /locus_tag="AC16UZ_01230"
     CDS             complement(528..2078)
                     /locus_tag="AC16UZ_01230"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: protein motif:HMM:NF004829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HIR6245328.1"
                     /translation="MSSYDVAIVGMGPVGAAAAILFADAGLKVIAFERDAHVYPLPRA
                     VALDGEVVRGFQRVGRAGELAALLQPVRPGERAGFANSRLEWLFGQPYATFGVNGWQP
                     ISMFDQPELEAHLRRLAMDHSGVTGCLEVQVETFEETGGQVAIRARQADGALSETSAR
                     FLVACDGAASGARRALEIGWRDLGYDHDWLVVDVVLKDGHTLGLDTLQVCDPDRLSTY
                     IATKDPFRRWEFKLNPGETREEMLEPERIRELIDPWTPRGTYEIRRSAVYQFHAATAE
                     TWRKGRVLLAGDAAHQTPPFLGQGLNAGMRDVINLAWKLPLVLSGAADEGLLDSYQAE
                     RGAHAADLVEWAVAIGKLMEHLAAVEAAQRVGRKPPEVPDGNRASGYGQGREAPPLRD
                     GVVMVDQVSEDGSTGYLFRQPAVRAADGEEYLLDDRLGAGFALVARSEADLAMSEASR
                     AIVDALGMSTTTLEGLEATRGRFDRLFEGSAAAVVRPDRYVFGHTSQSLSLDALLGEL
                     AARLGLRL"
     gene            complement(2075..2722)
                     /locus_tag="AC16UZ_01235"
     CDS             complement(2075..2722)
                     /locus_tag="AC16UZ_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6245329.1"
                     /translation="MPNQSHRGPVQERAQVTRDRLLDAAVRCFAAAGYEGSSTRQIEA
                     SAGVKRGLIAYHFGTKEALWKQAVTWWFGQAPHMLRVRVSGQGGSDPAAQLRALITGF
                     VRFSASFPEANRLMIREGMDDDWRLQWLAEHIVGPWYERLGAAFEEARSAGAVRNVPF
                     VHFYYILTGAGSLLFSMAPEAERLAGIDPYDDDVVRRHAEVIADLLIPGSGEHAH"
     gene            2721..3023
                     /locus_tag="AC16UZ_01240"
     CDS             2721..3023
                     /locus_tag="AC16UZ_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245330.1"
                     /translation="MDGKTLSFDELFYKSLVIRQVFIEEVAVSGMTDKLYARFRVPDL
                     NRQARSRSSRRWLGSQVFDYWKDPNGFTMEHFTDGDLFNASSGSEKWPVDVALGSH"
     gene            3103..4155
                     /locus_tag="AC16UZ_01245"
     CDS             3103..4155
                     /locus_tag="AC16UZ_01245"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6245331.1"
                     /translation="MLPGGLIFGTFLVLVLVLALVLVLVLVAGWYVWERRQRRTRAMP
                     GGLHEEITLPHRQAWELYHNNLSLCSKKTRACLSELSIDYESHHIDLIETGRYENVSR
                     EFLKVNPAGLVPVLVHEGHPVYESHAQIAFAAARSDDPHRLVPADPDQRRQMDDWVRK
                     TSLIGDDPVGAVHQTAGNAVPGLTLPLFATMLERIPLRRVLEGLLFHRLKRRAFILMI
                     IKLYGIDSLTSAKPLLRVVQASRDAMHRHLDDLEATLKDSGGPWIVGEHFSLADIGMM
                     AILERLREADWLDVLVSGRRPRVAAYWRALQTRPSYQSAIVACRGEAIERGTARLVAA
                     KQNNPVLRFALIETIT"
     gene            4203..5081
                     /locus_tag="AC16UZ_01250"
     CDS             4203..5081
                     /locus_tag="AC16UZ_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008094299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6245332.1"
                     /translation="MKLVSFSTTENPKPRLGAVRDGGIADLSGSDGIPETMLGLLHAG
                     DAAMKAAHEAAEAAADTVPLDQVTLHPPVLTPGKILAIGLNYGDHIEESGMGKPKHQV
                     WFNKQHNCVNGPYGDIALPSVSEMLDYEAELVVVIGKRCKHVPRERAAEVIAGYCCGN
                     DVSVRDWQMRAQTMQIGKSFDTHGPTGPWLVTPDEVGDPHDLDIRAEVNGELRQNSNT
                     KHLIYDCYDAVAHLTQAFTLDPGDLLFMGTPAGVGAAMKPPQFLKAGDVVRVEIEKLG
                     YLENRVVPEAAECVIE"
     gene            complement(5083..5682)
                     /locus_tag="AC16UZ_01255"
     CDS             complement(5083..5682)
                     /locus_tag="AC16UZ_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6245333.1"
                     /translation="MAQAQTHDHEIVSIYPFTDEEVDQLMNNSVDCVLMWATKDGWPV
                     GVTHAFVWHDGKIWLTFAAHRHRTVAIRRDNRVSVNVSSCGYPPGASEELPGGAITFK
                     GTGEFFDDEETKKWFYTALSQKLNPNSPEGEQFFYNLLDSPLRTVLAVTPVKKIMYNG
                     KLAGGHMAGTVKEEDLGPRLGVDKERMNAERAKRGLDPR"
     gene            5859..7082
                     /locus_tag="AC16UZ_01260"
     CDS             5859..7082
                     /locus_tag="AC16UZ_01260"
                     /EC_number="5.4.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR6245334.1"
                     /translation="MSNLRRSTQLYERAKAVIPGGIPGIRAPENFVPGAYPILLAGGR
                     GGHVTDVDGNDYVDLLLGFGPVILGHAEQAVDSAAHERAAHGFCLDLPEPVMIELAEQ
                     LVSMVPSAEQALLLKTGSDATSAAIRIARTHTGRSVVLRCGYHGWHDWCLQGDPGVPS
                     GTTDTVIAFPYNDLDRLAALLTAHSGDVATIMLTPVGHEFDRQIEQPEPGFLEGVRSL
                     ADQHGVVLAFDEVRTGFRVHPGGAQGLYGVIPDLTALGKAMSNGYAVTALVGRRDVMQ
                     SARSTFISSTYFPNGMEMAAALETLKLIAEHDVLTGIAATGAALAGGLSEIVARHGLP
                     VSLSPYDEMPFLHFDPDLASGQPERRDAFYGALARSGVFAHPRHHGFLCWRHSADDVT
                     RVLEAVDAAAAALGI"
     gene            complement(7079..>7699)
                     /locus_tag="AC16UZ_01265"
     CDS             complement(7079..>7699)
                     /locus_tag="AC16UZ_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR6245335.1"
                     /translation="RRLLGHGRLRPPNVGGDFLGARVYTDSARVELAPAELVSKLQAT
                     LASAREQGSASEFPFRLITKREVTTHNSWTHNYEGFLRGDQGRNYVFMNPTDAAALGL
                     KPDDLADVRSSAGAIRLPVRTSDDLLAGVVAVPHGWGHQASGQRVAAGSLGVNVNILA
                     SSGAAHVDPFSGMSHLTGLPVDVRLAADDRLAGNWSGIARPPGGPD"
BASE COUNT         1321 a         2627 c         2508 g         1243 t
ORIGIN      
        1 gggcgacctg gagatcgtgg tggattttct gtccgccgac gcggttcaga tcgagcccgg
       61 gcagcgggtc atcatcgagc gctggggcgg cgagtccgcc ctggtcggcc acgtgcggcg
      121 catcgagccc ttcgggttcc gcaagacgtc ggccctgggg atcgaggagc agcgggtgaa
      181 cgtgatcgtg gatttcacga gcccgcgcaa cgagtggtcg cgcctgggtc acggctatca
      241 ggtcgacgtg cgcgtggtgc tgtgggagca agacgcggtg ctcgcgatac cgctgacggc
      301 gctgtttcgc gacgatcagc ggtgggccgt gttcgtcgac gacgacggcc gcgccgccct
      361 gcgcgaggtc gtcgtcggcc accgcaacgg gatggtggcc gagatcgtca gcggtcttgc
      421 ccggggagac cgggtggttc tgcatccgag caaccgcgtg atcgacggcg tccggatcac
      481 cggcagaggc tgacgaggcc ggggggccta cggcgccgca gtagaggcta gagccgcagg
      541 cccagccttg ctgccagctc cccgaggaga gcatccaggc tcaaggattg cgaggtgtgt
      601 ccgaacacgt agcggtcggg tcgcaccacg gcagcggccg agccttcgaa gagcctgtcg
      661 aagcgtcccc gggtggcttc gagcccctcc agggtcgtgg tcgacatgcc gagggcgtcc
      721 acgatggccc gggatgcctc gctcatggcc agatcggcct cggacctggc aaccaacgcg
      781 aagccggcac ccagccgatc atccaggaga tactcctccc catcggcagc ccgcaccgca
      841 ggctggcgga aaagatagcc cgtggagcca tcctcgctca cctgatccac catcaccacg
      901 ccgtcgcgca ggggcggtgc ttcccgaccc tggccgtagc cggacgcgcg gttgccgtcc
      961 ggcacttcag gcggcttccg ccccaccctt tgtgccgctt ccacggcggc cagatgctcc
     1021 atgagcttgc cgatggccac cgcccattcc accaggtcgg cggcatgagc cccccgttcc
     1081 gcctgatagg aatccaggag accttcgtct gccgcaccgc tcaagaccaa cgggagcttc
     1141 caggccagat tgatcacgtc gcgcattccg gcgttcagcc cctgacccag aaagggcggg
     1201 gtctggtggg cggcgtcccc cgcgagcagc acgcgaccct ttcgccaggt ctcagcggtg
     1261 gcggcgtgaa actgatacac ggcgctgcgc cgtatttcgt aggtgccccg tggtgtccac
     1321 ggatcgatca gctcgcggat cctctcgggc tctagcatct cctcccgggt ctcgccggga
     1381 ttgagcttga actcccagcg ccggaaaggg tccttggtcg cgatgtaggt ggacagcctg
     1441 tcggggtcgc agacctgcag cgtatccaaa cccagggtgt gcccgtcttt cagcacgacg
     1501 tccaccacca gccagtcgtg atcgtagccc aggtcgcgcc agccgatctc cagggcccgg
     1561 cgcgcgccgc tcgccgcgcc gtcgcaggcg accaggaacc gggcgctcgt ctcggaaagc
     1621 gcgccgtccg cctgccgcgc gcgaatggcg acttgcccac cggtttcttc gaaggtctcg
     1681 acctggacct caaggcaccc agtcaccccg gagtggtcca tcgccaaccg gcggagatgc
     1741 gcctccagct ccggctgatc gaacatggag atgggctgcc agccgttgac cccgaacgtg
     1801 gcgtaaggct ggccaaagag ccactcgagc cgggagttcg cgaacccggc ccgctcgccg
     1861 ggccgaaccg gctgcagcag agcggcgagc tcgccggccc gccccacccg ctgaaagccg
     1921 cgaaccacct cgccgtccag ggcgacggcc cgcggcagcg ggtagacgtg cgcgtcgcgt
     1981 tcgaaggcga tgactttcag gcccgcgtcc gcgaagagga tggcggcggc agcgcccacc
     2041 ggccccatgc ccacaatggc gacgtcgtag ctgctcaatg tgcgtgctcc ccgctaccag
     2101 gaatgagcag atccgcaatg acttcggcgt gtctgcgcac cacatcgtcg tcgtacggat
     2161 cgatgcctgc aaggcgctcg gcctcgggag ccatggagaa gagaagggat cccgcccccg
     2221 tgagtatgta atagaagtga acgaagggga cgttgcgcac ggcccctgcg gatcgggctt
     2281 cctcgaacgc ggcgcccaaa cgctcgtacc agggcccgac gatgtgctct gcgagccact
     2341 gtagccgcca gtcgtcgtcc atgccctcgc ggatcatgag ccggttggcc tcggggaagc
     2401 tcgccgagaa gcgcacgaag ccggtaatga gcgcgcgaag ctgtgctgcc gggtccgagc
     2461 caccttgccc ggagacgcga acccgcaaca tgtgcggggc ctggccgaac caccaggtca
     2521 ccgcctgctt ccacagcgcc tccttcgtcc cgaagtggta ggcgatcaga ccacgcttga
     2581 ccccggcaga ggcctcgatc tgtcgcgtgg aagacccctc gtaacccgcc gcggcaaaac
     2641 agcgcacggc ggcgtcgagc aaccgatccc gggtgacctg ggcccgttcc tgaaccggcc
     2701 cgcgatggct ttgattcggc atggacggca aaaccttgtc atttgacgag ttattctata
     2761 aatcactcgt cattcgtcaa gttttcatcg aggaggtcgc cgtgtccggc atgaccgaca
     2821 aactctacgc gcgctttcgg gtccccgacc tgaaccggca ggcgcgctct cgatcttccc
     2881 ggcggtggct gggcagccag gtgttcgact actggaaaga tcccaacggc tttaccatgg
     2941 agcactttac ggacggcgat ctgttcaacg catcctccgg atccgaaaaa tggccggtgg
     3001 acgtggcttt ggggtctcac tgaggaccgg aaggagcgcc ttagggaaac gtcatgacca
     3061 gcgcgcgcga gatatcgacc caacgggctg cagagacctg aagtgctgcc gggcggtctg
     3121 atcttcggta ccttcctggt gctcgtcctg gtgctcgccc tggtgctcgt cctggtgctc
     3181 gtcgccggct ggtacgtgtg ggagcgccgg cagcggcgaa cgcgcgcgat gcccggcggc
     3241 ctgcacgagg agatcaccct cccccaccgg caagcctggg agctgtacca caacaacctg
     3301 tcgctctgct cgaagaagac gcgggcgtgt ttgagcgagt tgtccatcga ctatgaaagc
     3361 caccacatcg acctcatcga gaccggccgt tacgagaacg tctcccggga gttcctgaag
     3421 gtgaatcctg ccgggcttgt tcccgtgctc gtgcatgagg ggcacccggt gtacgaatct
     3481 cacgcgcaga tcgccttcgc cgcggcccgc agcgacgacc cccaccgcct ggttcccgcc
     3541 gaccctgacc agcgccgtca gatggacgat tgggtgcgca agacgtcgct catcggcgac
     3601 gaccccgtcg gcgcggttca ccagaccgcc ggcaatgccg tccccggtct gacgctcccc
     3661 ctgttcgcga ccatgctcga acggatcccg ctgcggcggg ttctcgaggg cctgctcttt
     3721 caccgactga agcggcgcgc cttcatcctc atgatcatca agctatacgg catcgacagc
     3781 ctgaccagcg caaagccact gctccgcgtg gtccaggcca gccgcgacgc catgcaccgc
     3841 cacctcgatg acctggaagc gaccctgaag gacagcggcg ggccctggat cgtcggcgag
     3901 cacttcagcc tcgccgacat cggcatgatg gccatcctgg agcgcctgcg cgaggctgac
     3961 tggctcgacg tgctggtgtc aggccggcgc ccccgggtgg ccgcctactg gcgcgcgctg
     4021 cagacgcgcc cgagctatca gtcggcaatc gtcgcctgcc ggggcgaggc gatcgaacgc
     4081 ggtaccgcca ggctcgtggc cgcaaagcag aacaaccccg tgctgcgctt cgccttgatc
     4141 gagacgatca cctgaacgcg cggccatcca tcagacacga caatcacgaa agcgaggaca
     4201 tcatgaagct ggttagtttt tccacgacag agaatccgaa accccgactc ggcgccgtgc
     4261 gggacggcgg gatagcggat ctgagcggtt cggacgggat cccggagacg atgctgggtc
     4321 ttctgcatgc cggggacgct gccatgaagg ccgcacacga ggccgctgaa gcggccgctg
     4381 acaccgtgcc gctcgatcag gtgacgctcc acccgccggt gctgacaccg ggaaagatac
     4441 tcgccatcgg gctcaactac ggcgaccaca tcgaggagag cggcatgggc aaacccaagc
     4501 atcaggtgtg gttcaacaag cagcacaact gcgtcaacgg accctatggc gacatcgcgc
     4561 tgccgtcggt ctccgagatg ctcgactacg aggccgagct ggtggtggtg atcggcaagc
     4621 gctgcaagca cgtgccgcga gagcgcgcgg cggaagtcat cgccggctac tgctgcggca
     4681 acgacgtgag cgttcgcgac tggcagatgc gcgcccagac catgcagatc ggtaagtcat
     4741 tcgacaccca cggccccacc gggccatggc tggtcacgcc ggatgaggtg ggcgaccccc
     4801 acgacctgga catccgcgcc gaggtcaacg gggaactccg ccagaactcg aatacgaagc
     4861 acctgatcta cgactgttac gacgccgtag cgcatctgac ccaggcgttc accctggacc
     4921 cgggcgacct gctgttcatg ggcacgccgg ccggtgtcgg ggcggcgatg aagcccccgc
     4981 agtttctcaa ggccggagac gtggtcaggg tggaaatcga gaagctcggc tacctcgaaa
     5041 accgggtggt ccccgaagca gccgagtgcg tgatcgagtg acctagcggg gatcgagccc
     5101 gcgcttggcg cgctcggcgt tcatgcgctc cttgtcgacg ccgagccgcg ggccgaggtc
     5161 ttcctctttg accgtcccgg ccatgtgccc gccggccagc ttgccgttgt acatgatctt
     5221 cttcaccggc gtgacggcaa gcacggtccg caacggcgag tcgagcaggt tgtagaagaa
     5281 ctgctcgccc tccgggctgt tgggattgag cttctgcgac agcgcggtgt agaaccactt
     5341 cttggtttcc tcgtcgtcga agaactctcc ggtgcccttg aaggtgatgg caccgcccgg
     5401 cagctcctct gaagcgcccg gcgggtagcc gcagctgctg acgttgacgg aaacgcggtt
     5461 atcgcggcgg atggcaaccg ttctatggcg atgcgcggca aaggtcagcc agatcttgcc
     5521 gtcgtgccac acgaacgcgt gggtgacccc taccggccag ccgtccttgg ttgcccacat
     5581 gagcacgcag tcgacggagt tgttcatcag ctggtcgact tcctcgtccg taaacgggta
     5641 aatcgagacg atctcgtgat cgtgagtttg cgcttgagcc ataagttccc cctggagtaa
     5701 ctgccgtccc cgatcccggg gaaaaagagc ggctagaata atgcaatcgc tcgatcggcg
     5761 ataccgacga gccccaccgg cctgctcgcg ccgatcggaa acagcaacgt cattccgctt
     5821 aaacttcaac cggtaaagga gcatccacgg taacgaccat gagcaacctg cgccgatcga
     5881 cccaactcta cgaacgcgcc aaggccgtca ttcccggggg catccccggc atccgggcgc
     5941 cggaaaattt cgtcccgggt gcttacccca tacttctggc cggaggccgc ggcggtcacg
     6001 tcacggacgt ggacggcaac gactatgtgg acttgctgct ggggttcgga cccgtgatcc
     6061 tcgggcacgc cgagcaggcc gtggacagcg cggcccacga acgcgccgcc cacggcttct
     6121 gcctggatct ccccgaaccc gtcatgatcg agctggccga gcagctcgta tccatggtgc
     6181 cctctgcaga gcaggcgctg ctgctgaaga cgggctccga tgccacgagc gctgcgatcc
     6241 gcatcgccag gacccacacg ggcaggtccg tggtcctgcg ctgcgggtac cacggctggc
     6301 acgactggtg tcttcaggga gatcccggcg tgccgtccgg caccaccgac accgtcatcg
     6361 cgttccccta caacgacctc gaccgcctgg ccgctctgct gacggcccac tcgggcgacg
     6421 tggcgaccat catgctcacc ccggtgggtc acgagttcga ccgccagatc gagcaacccg
     6481 aaccaggttt tctggagggg gtgcgcagcc tggccgacca gcacggcgtg gtgctcgcct
     6541 tcgacgaggt tcgcacgggc ttccgcgtcc acccgggcgg cgcccagggc ctctacggcg
     6601 tcatccccga cctgaccgct ctgggcaagg ccatgtcgaa cggttacgcg gtgacggccc
     6661 tggtcggccg gcgggacgtc atgcagagcg cccggagcac gttcatctca tccacctact
     6721 tccccaacgg catggagatg gcggcggctc tcgagaccct gaagctgatc gccgagcacg
     6781 acgtgcttac cggcatcgcc gctaccggcg ctgcgctcgc cggagggctg tcggaaatcg
     6841 tcgcccggca cggcctgccc gtctccctgt caccctacga cgagatgccc ttcctccatt
     6901 tcgacccgga cctggcatca ggccagcccg agcgacgaga cgctttctac ggcgccctgg
     6961 cccggagcgg cgtattcgcc cacccgcgcc atcacggatt tctctgctgg cgccacagcg
     7021 ccgacgacgt gacgcgcgtg ctggaggcgg tggatgccgc cgcggcggcg ctgggaatct
     7081 agtccggccc ccctggcggg cgggcgatgc ccgaccagtt tcccgccagc cggtcgtccg
     7141 ccgccaggcg gacgtccacg ggcaggccgg tgagatggga cattccggaa aacgggtcca
     7201 cgtgggcagc acccgagctt gcgagtatgt tcacgttcac gccgaggctg ccggccgcaa
     7261 cgcgctggcc gctggcctga tggccccagc cgtgggggac cgccaccacg ccggccagca
     7321 ggtcgtccga cgtccggacc ggcaggcgga tggcgcccgc ggagctgcgc acgtcggcca
     7381 ggtcgtcggg cttcaagccc agggcggcgg cgtccgtggg gttcatgaag acgtagttgc
     7441 ggccctggtc gccgcgcaga aacccctcgt agttgtgagt ccaggaattg tgcgtggtga
     7501 cctcccgttt ggttatcagc cggaagggaa actcgctggc agacccttgc tcgcgagccg
     7561 aggcgagggt ggcctggagc ttcgagacga gctccgcggg cgcaagctcc acccgcgccg
     7621 agtcggtgta gacccgcgcg ccgaggaaat cgcctccgac gttgggaggc cgcagccgac
     7681 cgtgaccgag cagccgtcg
//
LOCUS       DBNHOX010000058         1745 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1676194, whole genome shotgun sequence.
ACCESSION   DBNHOX010000058 DBNHOX010000000
VERSION     DBNHOX010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1676194"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="AC16UZ_01270"
     CDS             complement(<1..251)
                     /locus_tag="AC16UZ_01270"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6245336.1"
                     /translation="MDARPRERLGLGSGSRDRRRPRDTPREGPGVSAKSHPVVIVGAG
                     VSGLCCAIHLQDRGLRVVLLEADDEIGGRVRTDRIDGFLL"
     gene            complement(158..901)
                     /locus_tag="AC16UZ_01275"
     CDS             complement(158..901)
                     /locus_tag="AC16UZ_01275"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6245337.1"
                     /translation="MTSPTYLILGATGGMGQALCERLSKRGASLLIGGRDPVRLEELS
                     QRTGAESLAGDARDPLAIDRAVACTVERFGRIDGAVNLVGSLLLKPAHLTKAEEWDDV
                     VATNLGSAFHLVRAAAKAMRGQGGSIVLVSSAAARTGLVNHEAIAAAKAGIEGLARSA
                     AATYAAQGIRVNCVAPGLVRTPLTERLTRTPASLAASEAMHPMGRLGDPEDIASAIEW
                     MLDPANAWVSGQVLGIDGGLATLRGKARA"
     gene            1312..>1745
                     /locus_tag="AC16UZ_01280"
     CDS             1312..>1745
                     /locus_tag="AC16UZ_01280"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR6245338.1"
                     /translation="MARIGTQAPEWCTTAYHGGEEVELSSAKYRGKWYVLYWFPLDFT
                     FVCPTEIRGFQAMLGDFQAEGVEVIGASTDSFFSHKAWFADRKIFADEITHPVIADTN
                     HAVSRAFDVLKEDQGVAYRATAIVDDQGVIRSLSVNDLSAGR"
BASE COUNT          306 a          586 c          591 g          262 t
ORIGIN      
        1 agcaggaagc cgtcgatgcg gtcggttcgg acgcggcctc cgatttcgtc gtctgcctcg
       61 agcaggacaa cccggaggcc tcgatcctgc aggtggatcg cgcagcacag gcccgagacg
      121 ccggcgccca cgatcactac cgggtggctt ttcgcgctca cgcccgggcc ttcccgcgga
      181 gtgtcgcgag gccgccgtcg atcccgagaa cctgacccga gacccaggcg ttcgcggggt
      241 cgagcatcca ctcgatcgcc gaagcgatgt cctcgggatc accgaggcgg cccatcgggt
      301 gcatggcctc cgacgcagcg aggctcgcgg gcgtgcgggt gaggcgctcg gtcagggggg
      361 tgcgcaccag gcccggtgcc acgcagttca cgcggatgcc ctgtgccgcg taggtcgctg
      421 ccgcactccg ggcgagacct tcgatgccgg ccttcgctgc ggcgatggcc tcgtggttta
      481 ccaggcccgt gcgggccgcg gccgacgaaa cgaggacgat cgatccgccc tgtccgcgca
      541 tcgccttcgc agcggcgcgc acgagatgga aagcggagcc caggttcgtg gcgacgacat
      601 cgtcccactc ttccgccttg gtgaggtgcg cgggcttcag cagcagcgat cccacgaggt
      661 tcacggcacc gtcgatccgg ccgaagcgct cgaccgtgca agcgacggcc cggtcgatgg
      721 ccagcgggtc acgcgcatcc ccggccaggg actcggcgcc ggtgcgctga gagagctcct
      781 ccagacgcac agggtcgcgg ccgccgatca gcagggaggc gccgcgcttg gaaaggcgct
      841 cgcacagcgc ctggcccatg ccgcccgtcg cgccaaggat caggtaggtc ggggatgtca
      901 tcggattctc ctgtggacct gtttcgcaac tgagattaga acaagaatct agagatgtac
      961 agataaaatc ttgacaattt gctcacgtgg cctacgttgg gactcatggc gacttcggcg
     1021 gctgaaacga gcccgttccc cagggggaaa tcaccgagcc ccgaaccgct cccgcggccc
     1081 cgggcgacgc gggctgaggc ccgccgccag ctcctcaacg agctcgcgag cggggccagc
     1141 ggcctggtcc tcgccgtctt cgtgtggggg acgccgcatc ctgcgacgca ccgcaacgca
     1201 ctggcgactg gaatgtcgca gccccgggag ctgctccaga tccagacgta ggcgcaggtc
     1261 gccgggtgaa cggcaaccgt catcaggatc accgaggaaa ggagagatga catggcacgg
     1321 atcggaaccc aggcaccgga atggtgcacc acggcctatc acggaggtga ggaggtcgag
     1381 ctctcgagcg cgaaataccg tggcaagtgg tacgtcctct actggttccc gctggacttc
     1441 acgttcgtct gcccgaccga gatccgaggc ttccaggcga tgctcggtga cttccaggca
     1501 gaaggcgtcg aggtcatcgg agccagcacc gactcgttct tcagccacaa ggcatggttc
     1561 gcggaccgga agatcttcgc ggacgagatc acccaccccg tgattgcgga caccaaccac
     1621 gctgtgagcc gcgccttcga cgtcctcaag gaggaccagg gcgtggccta ccgggcgacg
     1681 gcgatcgtcg acgaccaggg cgtcatccga tccctgagcg tcaacgacct gagcgccggc
     1741 cggag
//
LOCUS       DBNHOX010000059        12265 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_519077, whole genome shotgun sequence.
ACCESSION   DBNHOX010000059 DBNHOX010000000
VERSION     DBNHOX010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12265
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_519077"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="AC16UZ_01285"
     CDS             complement(<1..311)
                     /locus_tag="AC16UZ_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245339.1"
                     /translation="MPQHPREPDIASWHRYFAIECNNVAWDLAGRARTAPEDEQMLQA
                     AHAAAFHWDQVGEELNRMRATMLLAKVHALLGKNPSALRYAEEMQTFFLDRDTPDWEI
                     A"
     gene            complement(351..2030)
                     /gene="ilvD"
                     /locus_tag="AC16UZ_01290"
     CDS             complement(351..2030)
                     /gene="ilvD"
                     /locus_tag="AC16UZ_01290"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017338119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR6245340.1"
                     /translation="MTDQKRPYSSIVVDGVQQAPSRAMLYPVGFTEADFAKPQIGIAS
                     TWSMVTPCNMHIDALAEAAALGADEAGGKSVVFNTITVSDGISMGTPGMRYSLVSREV
                     IADSIETVVGAQGFDGFVAIGGCDKNMPACAMAMARLNRPSVFVYGGTIQPGAERRDI
                     VSVFEAVGAHAAGRISDIELKEVEATAIPGPGSCGGMYTANTMASAIEAMGLSLANSS
                     AQEAISNAKRQDSYQAGAAVFNLIERGIKPSDILCREAFENAITVAIALEGSTNAVLH
                     LLAIAHAAGVPLELDDFSRVGERVPVLADMRPAGQYAMSELIAIGGIQPLMKTLLEEG
                     LLHGDCLTVTGKTLAENLADVAPYPDGQNVIRPMSDPIKKDSHLVVLYGNLAPTGAVA
                     KITGHEGLSFTGSAKCFHGEEAAMAAIMDGAINKGDVVVVRYEGPKGGPGMREMLSPT
                     SAINGRGLSQDVALITDGRFSGGSHGFVIGHITPEAYEGGPIALIEDGDLITVDAEKR
                     EINVQLSDADLAARRARWQKPAPYATKGILAKFARLVSSASEGAVTDKNLD"
     gene            complement(2063..3394)
                     /gene="argA"
                     /locus_tag="AC16UZ_01295"
     CDS             complement(2063..3394)
                     /gene="argA"
                     /locus_tag="AC16UZ_01295"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003641.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-CoA as donor [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HIR6245341.1"
                     /translation="MSDRPAKAEAENGYARWFRASTPYISAHRNRTFVILLGGDAVAH
                     GNLPNIVHDLALLHVLGARVVVVHGARPQIDAALGESPFHEDRRITDAAAMETIIGIL
                     GQQRARLEALFSTGLPTSPLHNTNISVVGGNFITARPVGIHDGVDHLFTGRPRRVHGG
                     RIQKALDTGALVLLSPLGYSPSGQAFNLAADELAADVAMALDADKLIAFGSDRYVSNE
                     RGEPQPNLTPAQLDTLIDVPALDDTTTRGHLRALLRACRGGVPRCHLVSYAHDGALLE
                     ELFTAGGGGTQISEDHSNLVRQARLEDVAGIVELIRPMEEAGVLVRRPRDRLEQEIDR
                     FFVAEVDGIVMGCCALYPFEDCGELACVAVHESHRSKPGETRLGVALLRATEAAAGAM
                     GLRKLFVLTTQTRDWFVDNGFEDSTLDALPAPKQAMYNYQRNSKVMIKQLR"
     gene            3713..5482
                     /gene="tfpB"
                     /locus_tag="AC16UZ_01300"
     CDS             3713..5482
                     /gene="tfpB"
                     /locus_tag="AC16UZ_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly ATPase TfpB"
                     /protein_id="HIR6245342.1"
                     /translation="MPLAQEQLIDLPVLLDGLVKEGYISQRQAEDILITPRTKKELSQ
                     HPMEIVADRDFDNRNKPGHKLDLETMTVWLAHQSGQPYLRIDPLDINVGAVTDVMSYA
                     FAQRHNILAVDVTDDEVVIASAQPYMYQWETMLTQTLRGRRIRRVVTNPADLSRYMLE
                     FYTMARSVAKAQDAGMEMSGLANFEQMLELGQMKSPEANDSHIVNIVDWLLQYAFDQR
                     ASDIHIEPRRDKGLIRFRIDGVLHHVYELPQQVNAAVTSRLKILGRMDVAEKRRPQDG
                     RVKTLSPDGDEIELRLSTLPTAFGEKMVLRIFDPDVLLRSFKELGLSGDDYERWHGMT
                     HSAHGIVLVTGPTGSGKTTTLYSTLKQLATSEVNVCTIEDPIEMVEPAFNQMQVQSNI
                     NVTFAAGVRALLRQDPDIIMIGEIRDLETAEMAIQAALTGHLVISTLHTNDAPTAITR
                     LLDLGVPPYMIKATVLGIMAQRLVRTLCPHCKVEEETDAEAWRLLTKPFRAKQPERVF
                     APKGCLECRDTGYMGRVGIYELLPMSEKVQTLVQADTDMDVLRKQAFKDGMLTLRLSG
                     AQKVGSGLTTISEVLRVAPASQD"
     gene            5487..8231
                     /gene="glnE"
                     /locus_tag="AC16UZ_01305"
     CDS             5487..8231
                     /gene="glnE"
                     /locus_tag="AC16UZ_01305"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HIR6245343.1"
                     /translation="MSSSSVPPELLSKAERTAARLPYPLPADGLRVLALSDFAANVMA
                     REAQWIQDALEADRFSAPLASGEVRATLAAVSQGAEDLGALQQSLRQWRNRYQLWVVW
                     RHLLRMAPLEETCATLSMLADTMLDLALERLYQWATDRQGVPMGESSRSPQRLVVLAL
                     GKLGARELNLSSDIDLVFAYPEPGATTSGTTNQQFFLRLGQQLIQALDAVTADGFAFR
                     VDMRLRPFGASGPLVMHFEGMEDYFAAHGRDWERYAWIKARACAGDVASGEALLQSLR
                     PFVYRRYLDFGAVDALRDMKRRLYSERNDPNDVKLGPGGIRDVEFVVQLQQMVWGGRQ
                     PELQEANLLRVLPRLASQGHLSDADAAALAEGYRFLRDTEHSIQAEGDLQTQSLPASE
                     DSRQRLASSRGFADYGAFESALNGHRIAVEAVFRNAVGELPEEDTPGQRLWSSPDDAA
                     LLGELGFARAEAAGKLLTSLMEARDRPSQSAESRARLDRLMPGLLDRVSLTEDPDLSL
                     ARVVPVLRSVLRRSAYLSLLRENPHTLAHFVDLTAKSLWLAEQLARFPAFFDALLDER
                     QLAALPDKAALQAELHQQVEAAPADDFERRLDVLREFKEHHVFNVSLAEVRGTLPLMK
                     ASDYLTFLAEAVLGESLDLAWNENADRFPEFAEPRPFIIVGYGKLGGIELGPGSDLDL
                     VFIHDLPVGASQFLHRMVRRLLHILTVPTYSGTLYEIDMRLRPSGSSGTMVSSLEAFA
                     EYQRSQAWMWEHQALVRARAVAGDPALAQRFEAVRRELLCEPRDRGPLREEVLKMRAR
                     MAEHHGDAEDLKRGSGGIVDIEFMVQYLVLAWAHEHPALAEFSDNVRILETAERLDLL
                     PANMARNLREAYVGLRSEWHRSVLDLPDSERAARTLMAHRSDVRDAWREVFGIDPENE
                     "
     gene            8242..9183
                     /locus_tag="AC16UZ_01310"
     CDS             8242..9183
                     /locus_tag="AC16UZ_01310"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR6245344.1"
                     /translation="MTEPTFSDRDGWIWHNGELVPWRDAKVHVLTHTLHYGVGVFEGV
                     RAYQTPSGPAIFRLHEHTRRLFNSAHILGLKIPFSVDEVNAAQVDVFRANALNEGYLR
                     PMVYLGSEAMGLRAKDLSVNLCVAAWAWPDYMDPDSREKGINVRTSSFTRHHVNITMC
                     KAKANGNYINSILALHEALDSGCDEALLLDNEGYVAEGSGENVFIVKDGEVHTPELTS
                     CLDGITRNTIFHLAAEMGIAIRERRITRDEVYIADEAFFTGTAAEVLPIRELDGRMIG
                     SGKRGPVTERFQSAYFDQVRGKRSAFPEWHTPVSGAQ"
     gene            9173..10177
                     /gene="waaF"
                     /locus_tag="AC16UZ_01315"
     CDS             9173..10177
                     /gene="waaF"
                     /locus_tag="AC16UZ_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017336199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HIR6245345.1"
                     /translation="MPNSGTRILIVAPAWVGDMVMAHSLVQVLIARHGDPGIDMLAPR
                     ATAGLGARMPGVRNVHVLDVEHGELALGARRSSGLTLKDQYDQAIVLPNSFKSALAPA
                     WARIPVRTGWSGEARVGVLNDRRRLNPERYPRMVDRFAALGLDPDAPLPDDIPGPVLE
                     PDAAAAERLLASRRLSVDGGVTALCPGAEFGPAKRWPSVHFAQVASYAAAQGHQVWLV
                     GSPKDAGVCSEVEAQSPAGVVNLAGRTTLLEAVDLLSRADRVVSNDSGLMHVASALGR
                     PVVGVFGSTSTTFTPPLGERATTVSLGIECSPCFQRECPLGHLRCLNDLPPQRVIELL
                     "
     gene            10177..11232
                     /gene="waaC"
                     /locus_tag="AC16UZ_01320"
     CDS             10177..11232
                     /gene="waaC"
                     /locus_tag="AC16UZ_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008016956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HIR6245346.1"
                     /translation="MHVLLIKMSSMGDVVHALPAVTDAASHGVTFDWVVEEAFADIPA
                     LHPAVGQVMPIAWRRWRSSLRSSRGELARFWEQLRRRRYDLVLDSQGLVKSGVVAALA
                     RGRTKAGFSHASARERAAALAYRRGVRVARNQHAIDRQRELFAAVFGYCVPAGLDYGI
                     ARAAHQASKTNHCVLLHGTTWESKHYPEHLWREVARRARAAGLDVALPWGSEAERARA
                     EAIATTNADVLPALNLAELTTRIASARLVVGVDSGLAHLSAALDVPTVVIYGSTDSAL
                     TGCRGRRVSNLQAQFPCAPCLRRACGYHGPEEILHQAPVRPACYSRVTPDAVWDAAAR
                     AMADGGRETAPNPGSDL"
     gene            11229..>12265
                     /locus_tag="AC16UZ_01325"
     CDS             11229..>12265
                     /locus_tag="AC16UZ_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BUD32 family EKC/KEOPS complex subunit"
                     /protein_id="HIR6245347.1"
                     /translation="MTATGRPLSLAELQAQGRDLPTPFILEGSLNPAPDDGPIECLEV
                     LRVLPGRRIVMRARLGARQCALKLFVGDRAALSARRERAGLELLAASGVDTPELIADL
                     HLPGGGEGILVDWLAGSRPVDEEDVQGLADVAAVLARLHANGAIQHDAHLNNYLRDAD
                     GMLHAIDGDGVRRPARLRRRQELANLALLLAQLPPPDATRLPVVAEAYWRARWGSDPG
                     PDELAALQRRVHAQRAGRMRRYLRKTLRDCSEFHGERKNGRFVVCARNAWTAQMAEFV
                     DRPEDHLVAGTVLKAGNSATVMRLRIGETAYVVKRYNVKGFFHGLRRALRPTPRYRVA
                     WLSGHRLLRLG"
BASE COUNT         2028 a         3998 c         4072 g         2167 t
ORIGIN      
        1 aaggcgatct cccaatcggg cgtgtcccgg tccagaaaga aggtctgcat ttcctcggcg
       61 taccgcagcg cggaggggtt cttgcccaga agcgcgtgca ccttggcgag cagcattgtg
      121 gcccgcatcc tgttgagctc ctcgccgact tgatcccagt gaaaggccgc ggcatgcgcg
      181 gcttgcagca tctgctcatc ctcaggcgcc gtgcgggcgc ggccggccag gtcccaagcc
      241 acgttgttgc actcgatggc aaagtatcga tgccatgatg cgatgtcggg ctcccgcgga
      301 tgttgcggca cgtctgctta tccccgtttc acggcatgct gccgatgggc tcaatccagg
      361 ttcttgtcgg taacggcgcc ttcactcgcc gagctgacca gccgggcgaa cttcgccaga
      421 atgcctttcg tggcgtacgg cgccggtttc tgccagcggg cgcggcgcgc ggccaggtcc
      481 gcgtcgctca actggacgtt gatctcacgc ttctcggcat cgacggtgat cagatcgccg
      541 tcctcgatca gcgcgatggg ccctccctcg taagcctcgg gcgtgatgtg accgatgacg
      601 aagccgtggg agccgccgga gaaacgcccg tcggtgatca gcgccacgtc ctgactaaga
      661 ccgcggccat tgattgcgct ggtgggcgag agcatctcgc gcatccccgg gccgcccttc
      721 ggcccttcgt agcgtacgac caccacgtcg cccttgttga tggccccgtc catgatggcg
      781 gccatggcgg cctcctcgcc gtgaaagcac ttcgcgctgc ccgtaaagga caacccctcg
      841 tgtccggtga tcttcgccac ggccccggtg ggggccaggt tgccgtagag aacgaccagg
      901 tgggaatcct tcttgatcgg gtcgctcatg ggacggatga cgttctggcc atccggatac
      961 ggggccacgt cagcgaggtt ctcggcgagg gtctttccgg tgaccgtgag gcagtcgccg
     1021 tgcagcaggc cctcttcgag cagcgttttc atgagcggct ggatgccgcc gatggcaatg
     1081 agctcactca tggcgtactg gccggccgga cgcatgtcgg ccagcacggg aaccctctcg
     1141 ccgacgcggg agaaatcgtc cagctccagg ggcacgccgg ctgcgtgggc gatggcgaga
     1201 aggtgcagaa cggcgttggt cgatccttcc agggcgatgg cgacggtgat ggcgttctcg
     1261 aacgcctcgc ggcagagaat gtcgctgggc ttgatcccgc gctcgatcag gttgaacacc
     1321 gccgcgccag cctggtagct gtcctggcgc ttcgcattcg agatcgcttc ctgggctgag
     1381 ctgttcgcca gcgacaaacc catagcctcg atggcggatg ccatggtgtt cgccgtgtac
     1441 atgccgccgc aggaaccggg ccccgggatc gccgtggcct cgacttcctt gagctcaata
     1501 tcggagatcc gtcccgccgc gtgagcgccc acggcctcga ataccgagac gatgtcgcgt
     1561 ctttcggcgc cgggctggat ggtcccgccg tagacgaaca cgctcggacg gttcaggcgg
     1621 gccatggcca tcgcgcaggc gggcatgttc ttgtcacaac cgccgatggc gacgaacccg
     1681 tcgaatccct gggcgccgac gacggtctcg atggagtcgg cgatcacctc ccgcgaaacc
     1741 agcgagtaac gcatacccgg cgtgcccatg ctgatgccgt cggaaacggt gatcgtgttg
     1801 aacaccacgc tctttccgcc ggcttcgtcg gcgccgagcg ctgccgcctc ggccagggcg
     1861 tcgatgtgca tgttgcacgg agtgaccatg ctccacgtgg aggcgatccc gatctgtggc
     1921 tttgcgaagt ccgcctccgt gaaacccacg gggtagagca tcgcgcggct cggagcctgc
     1981 tgcacgccgt ccacgacgat ggacgagtag ggtcttttct ggtcagtcat ggagttcccc
     2041 tggggcccgg cctcactgcc ggttacctga gttgcttgat catcaccttc gagttgcgct
     2101 ggtagttata catagcctgc ttgggcgctg gaagcgcgtc gagagtggaa tcctcgaagc
     2161 cgttgtccac gaaccagtct cgggtctggg tggtcagcac gaagagcttc ctcaggccca
     2221 tcgcgcccgc tgcggcttcc gtcgccctta agagcgcaac cccgaggcgg gtctctccag
     2281 gcttgctgcg atgggattcg tggacggcca cgcacgcgag ctccccgcag tcctcgaagg
     2341 gatacagagc gcagcaaccc atcacgatgc cgtccacctc ggcaacgaag aaacgatcga
     2401 tctcctgctc cagcctgtca cgcggacgcc ttaccagcac gccggcctcc tccatgggcc
     2461 tgattagctc gacgatgccg gccacgtctt cgagccgcgc ctgccgcacg aggttcgagt
     2521 gatcttcgct gatctgggtg ccgcctccgc cggccgtgaa gagctcctcc agcagcgcgc
     2581 cgtcatgcgc gtagctcacc aggtggcaac gcggcactcc gcctcgacag gcgcgcagaa
     2641 gcgctcgcag atgaccgcgg gtggtcgtgt catccagggc cggaacgtcg atcagggtat
     2701 cgagctgggc gggtgtcagg ttgggttgcg gctcgccgcg ttcgttgctg acgtagcgat
     2761 cgctgccaaa ggcgatcagc ttgtcggcat ccagggccat ggccacgtct gccgcgagct
     2821 cgtccgcggc caggttgaac gcctgcccgg aaggcgagta gcccagggga gacaacagca
     2881 cgagcgcgcc ggtatccagg gctttctgga ttcgcccccc gtggactcgt cgcggccggc
     2941 cggtgaacag gtggtccacg ccgtcatgga tgccgacggg tcgcgccgtg atgaagttgc
     3001 caccgaccac ggagatgttc gtgttgtgca gtgggctggt gggcagcccg gtggagaaga
     3061 gggcctccag tcgggcgcgc tgctgtccca ggatgccgat gatcgtctcc atggcggctg
     3121 cgtcggtgat gcggcgatcc tcgtgaaagg gcgattcccc tagggccgca tcgatctgcg
     3181 gccgtgcgcc gtgcaccact accacccgtg cacccaggac gtgcagcagc gcgagatcgt
     3241 gaacgatgtt cggcaggttt ccatgggcga cggcgtcgcc cccgagaagg atgacgaacg
     3301 ttcggttgcg gtgcgcgctg atgtaggggg tcgacgccct gaaccatcgt gcgtatccgt
     3361 tctctgcttc tgccttggcc ggtctgtcgc tcatgaattg ctgccctgcc gccgcccccc
     3421 tcgggaagcg cgtcgtcaaa aaagggggcg cattttacac cagcgacctc gaatggcatg
     3481 gcaaacgtga cgccggtcta attccgaaaa tgccatcgcc ggctctgggc gctagccggt
     3541 tgcaaaagcc gcatccttgt cattaactga aacttcttgg ctaatatcag ggacttgcgt
     3601 ggccaacccc ctcattgatt ctccggcggg cagggatccg cgtccgctgt cggagggagc
     3661 tgctccgttg gcagccgggt cggagccgaa ggtcgcctcc cttgccagcc gcctgccgtt
     3721 ggcgcaggag cagctcatcg acctgccggt gcttctcgac gggctcgtca aggaaggcta
     3781 catcagccag cgtcaggcgg aagatatcct gatcacgccg cgcaccaaga aagagctcag
     3841 ccagcatccc atggagatcg ttgcggatcg cgatttcgac aaccgtaaca agcctggcca
     3901 caaactcgac ctcgagacca tgacggtctg gctggctcat caaagcggtc agccctatct
     3961 gcgcatagat ccgttggaca tcaatgtcgg cgcggtcacg gacgtgatgt cgtacgcctt
     4021 tgcgcaacgg cacaacatcc tcgccgtgga cgtgaccgac gacgaagtcg tcatagccag
     4081 cgcccagccc tacatgtacc agtgggagac catgctcacc cagacgctgc ggggccggcg
     4141 catcagacgg gtggtgacta accccgcgga cttgagccgc tacatgctcg agttctacac
     4201 catggcccgc tcggtggcga aggctcagga tgcggggatg gagatgagcg gcctggcgaa
     4261 cttcgagcag atgctcgagc tcggccagat gaagtctccg gaagccaatg attcgcacat
     4321 cgtcaacatc gtcgactggc ttcttcagta cgcgttcgac cagcgggcca gcgacattca
     4381 catcgagccg cgtcgcgaca agggactgat ccgttttcgc atagacggcg ttctgcacca
     4441 cgtctacgag ttgccgcagc aggtgaacgc ggcagtcacc agccggttga agattctggg
     4501 ccgaatggac gtggctgaga aacggcgccc tcaggacggt cgcgtaaaga ccttgagccc
     4561 ggatggcgac gagatcgagc tgcgcctgtc gaccctgccc acggcgttcg gcgaaaagat
     4621 ggtgctccgt atctttgatc cggacgtgct gctgcgcagc ttcaaggagc tcgggctgtc
     4681 cggcgacgat tacgagcggt ggcacggcat gacccactct gctcacggca tcgtcctggt
     4741 cacgggcccg accggctcgg ggaagacgac gaccctgtac tcgacgttga agcagctggc
     4801 cacttccgag gtcaacgtct gcaccatcga ggaccccatc gagatggtcg agcccgcctt
     4861 caaccagatg caggtgcagt cgaacatcaa cgtcaccttc gccgccggcg tgcgcgccct
     4921 tctccggcag gacccggaca tcatcatgat cggtgagatt cgtgatctcg agacggcaga
     4981 gatggcgatt caggcggctc tcaccggcca cctcgtcata tccacccttc acaccaacga
     5041 cgccccaacc gcgatcaccc gtctgctcga tctcggggtt cccccttaca tgatcaaggc
     5101 caccgttctc ggtatcatgg cccagcgtct ggtgcgtacg ctctgtcctc actgcaaggt
     5161 cgaagaagag accgatgcgg aggcttggcg acttctgacc aagccgttcc gcgcaaagca
     5221 gccggagcgg gtgttcgcac ccaaggggtg tctggagtgc cgggacacgg ggtacatggg
     5281 acgggtgggc atctatgagc tgttgccgat gagcgagaag gtccagaccc tggttcaggc
     5341 agacacggat atggatgttc tgcgcaaaca ggctttcaag gacggcatgt tgacgctccg
     5401 cctgtcgggt gcgcagaagg tcggctctgg cctgaccacg atttcggagg tcctgcgggt
     5461 ggctccggcc tcccaagact gaacatttgt cctcttcttc ggtaccccca gaactgctga
     5521 gcaaggccga gcgaacggct gcccgacttc cgtatcccct tcctgccgac gggctgcggg
     5581 tgctcgcgtt gagcgacttc gccgccaacg tgatggcgcg ggaggcccaa tggattcagg
     5641 acgcgctcga ggccgaccgc ttctccgccc ccctcgcgtc gggggaagtc cgcgcgacgc
     5701 tcgccgccgt ttcccaaggg gccgaggacc taggcgccct gcagcagagc ctgcggcagt
     5761 ggcgcaaccg ctatcagctc tgggtggtct ggcgccacct cctgcggatg gcgccgctgg
     5821 aagaaacctg cgccaccctc tccatgctgg cagacaccat gctggatctc gccctggagc
     5881 gtctttacca gtgggccacg gacaggcaag gcgtgccgat gggggagtcg agccgatcgc
     5941 cccagcgcct ggtggtgctg gcgttgggga aactcggtgc ccgtgagctg aatctctcgt
     6001 cggatatcga tctggtattc gcctacccgg agcccggcgc cacgacgagc gggaccacca
     6061 accagcagtt cttcctacgc ctgggtcagc agctcattca ggcgctggat gcggtgacgg
     6121 cggacggctt tgcctttcgc gtcgacatga ggctccggcc atttggcgcc agcgggcccc
     6181 tcgtcatgca cttcgagggc atggaggact acttcgccgc tcatggcagg gactgggagc
     6241 gctatgcctg gatcaaggcg cgagcctgcg caggggacgt agcgtcggga gaggccctgc
     6301 ttcagtccct gcggcccttc gtgtacaggc ggtatctcga tttcggggca gtagacgcgc
     6361 tgcgcgacat gaagcgcagg ctctactccg agcgtaacga tcccaatgac gtcaagctcg
     6421 ggcccggcgg gatcagggat gtggagttcg tcgttcagct gcagcagatg gtctggggcg
     6481 ggaggcagcc ggagctgcag gaagccaacc tgctgcgtgt cctgccgcgg ctcgcctccc
     6541 agggccatct gagcgacgcg gacgcggccg cgctggccga aggctatcga ttcctgcgtg
     6601 acaccgagca cagcatccag gccgagggcg accttcagac ccagtcgctg ccggcgtccg
     6661 aagacagccg ccagcggctt gccagctcca ggggctttgc cgactacggc gcgttcgaaa
     6721 gcgcgctgaa cgggcaccgg atcgcggttg aagccgtctt caggaacgcg gtgggggagc
     6781 tgccggagga agacacgccc ggccagcgcc tctggtcaag cccggatgac gccgcgctgc
     6841 tcggcgaatt gggtttcgcg cgagccgagg cggcgggcaa gctgctgacc agcctgatgg
     6901 aagcgcgcga tcggccctcc cagagcgccg aaagcagagc ccggctggac cggctcatgc
     6961 ccggccttct cgaccgggtg agccttacgg aggatccgga tctctcgctt gcccgcgtgg
     7021 tgcccgtgtt gcgatccgtg ctgcggcggt cggcctatct gtcgctgctc cgcgaaaacc
     7081 cccacaccct tgctcacttc gtcgacctga cggccaaaag cttgtggttg gccgagcagc
     7141 tcgcccgctt tccagcgttc ttcgacgcgc tgctggacga acgccagctc gctgcgcttc
     7201 cggacaaggc cgcccttcag gccgagctgc accagcaggt cgaggcggcg ccggcggatg
     7261 acttcgaacg tcggctcgac gtgctcaggg agttcaagga gcatcacgtg ttcaatgtct
     7321 ccctggcgga ggttcgtgga acgctgcccc tgatgaaggc gagcgactac ctcacgtttc
     7381 tggccgaggc cgtgctcggc gagtcgctgg acctggcctg gaacgaaaac gccgaccgct
     7441 ttcccgagtt cgccgagcct cgacctttca tcatcgtcgg ctacggcaag ctcggcggta
     7501 tcgagctcgg gccgggaagc gacctggacc tggtcttcat tcacgacctg cccgtcggcg
     7561 cctcgcagtt cctgcaccgg atggtccgcc gcctgcttca catcctcacc gttcccacct
     7621 attccgggac gctttacgag atcgacatgc gcctgcgccc ttcgggcagt tcgggaacca
     7681 tggtttcgag cctggaggct ttcgccgagt accagcgatc ccaggcctgg atgtgggagc
     7741 atcaggcgct ggttcgggcg cgcgcagtgg ctggcgatcc ggcgctcgcc cagcgcttcg
     7801 aggcggttcg acgggaactt ctgtgcgagc cccgggaccg tgggccgctg cgcgaggaag
     7861 tgctgaagat gcgagcccgc atggccgagc accatggtga cgcggaagac ctcaagcgag
     7921 gctccggcgg tatcgtcgat atcgaattta tggtgcaata cctggtcctg gcctgggcgc
     7981 acgaacaccc tgcgctcgct gaattctcag acaacgtgcg cattctagag acggctgagc
     8041 ggctggatct gcttcccgcg aacatggccc ggaacctccg ggaggcttac gtgggtctgc
     8101 gctctgagtg gcaccggtcg gtgcttgacc tgccggacag cgagcgggct gcccggaccc
     8161 tgatggccca caggtccgac gtgagggatg cgtggcgcga ggtgtttggt atcgacccgg
     8221 aaaacgagta agcgcggaat catgacagaa ccgacatttt cggaccgaga cggctggatc
     8281 tggcacaacg gggaactggt gccctggcgt gacgcgaaag tccacgtgct gactcacacg
     8341 ctgcactacg gcgtgggagt gttcgaaggc gtgcgggcgt atcagacccc ctcgggcccc
     8401 gctattttca ggcttcacga gcatactcgc cggcttttca actcggcgca catcctcggg
     8461 ctcaaaatcc ccttcagcgt cgacgaggtc aacgcagccc aggtcgacgt tttccgtgcc
     8521 aacgctctga acgaaggcta cctgcggccc atggtgtacc tgggttcaga ggccatgggc
     8581 ctgcgggcca aggatctttc ggtaaatctc tgtgtcgccg cctgggcgtg gccggattac
     8641 atggaccccg acagccgcga gaaggggatc aacgtgcgga cgtcgtcctt cacccgccac
     8701 cacgtgaaca tcaccatgtg caaggcgaaa gccaacggca actacatcaa ctccattctc
     8761 gctcttcatg aggcgctgga cagcggatgc gacgaggcgc tgctgctcga caacgaggga
     8821 tacgtcgccg agggcagcgg cgagaacgtc ttcatcgtca aggatggcga ggtgcacacg
     8881 ccggagctta cctcctgtct cgacggcatc acgcgaaata cgatcttcca tctggccgcg
     8941 gagatgggca tcgccatcag ggagcggcgc atcaccagag acgaggtgta catcgccgac
     9001 gaggcgttct tcacggggac ggccgccgag gtgctgccga ttcgcgagct cgatggtcga
     9061 atgatcggct cgggcaaacg cgggcccgtc acggagcggt tccagtcggc ctacttcgac
     9121 caggtgcggg gcaaacgcag cgccttcccg gagtggcaca cgcccgtaag cggtgcccaa
     9181 tagcggcacc cgcatcctga tcgtcgcccc cgcttgggtt ggcgacatgg tcatggcgca
     9241 cagcctcgtg caggtcctca tcgcgcggca cggcgacccc gggatcgata tgctagcccc
     9301 tcgcgccacg gccgggctcg gagcccgcat gccgggcgtg aggaacgttc acgtgctcga
     9361 cgtggagcac ggtgagctcg ccctgggcgc caggcgcagc tcggggctga ccctcaagga
     9421 tcagtacgac caggccatcg tgctgccgaa cagcttcaag tcggcgctcg cacccgcgtg
     9481 ggccaggatt cccgttcgca ccggctggag cggcgaagca cgggtcgggg tgctcaacga
     9541 ccggcgccgc ctgaacccgg agcgctaccc gagaatggtc gaccggttcg cggctctggg
     9601 actcgatccc gacgcgccgc tgccggacga tattcccggt cccgtcctcg agcccgatgc
     9661 cgctgccgcg gagcgcctgc tcgcctcccg ccggctctcg gtggacgggg gcgtgaccgc
     9721 cctgtgcccg ggggccgagt tcggcccggc caagcgctgg ccgtccgttc actttgccca
     9781 ggtcgccagc tacgccgccg ctcaaggaca tcaggtatgg ctcgtggggt cgccgaaaga
     9841 tgccggggta tgctcggagg tggaggcgca gtcgccggcc ggcgtggtca acctcgcggg
     9901 gcgcaccacg ctgctggaag ccgttgacct gctctccagg gccgaccggg tcgtcagcaa
     9961 cgattcgggt ctcatgcacg ttgcctcggc gctcggccgg cctgtggttg gcgtcttcgg
    10021 atcaacctcc acgaccttca cgccgccgct cggcgagcga gcgacgacgg tgagcctggg
    10081 tatcgagtgc agcccgtgct ttcaacgcga atgcccgctg gggcatctgc gctgcctcaa
    10141 cgacctgccg cctcaacgag tcatcgagct gctctagtgc acgtcctgct gatcaagatg
    10201 tcgtcgatgg gggacgtggt ccatgctctg cccgcggtga cggacgccgc gagccatggg
    10261 gtgactttcg actgggtagt tgaggaggcg tttgcggaca tacccgcgct gcacccggca
    10321 gtcggccagg tgatgcccat tgcctggcgg cgatggcgct cctcgctgcg ctcgtcccgg
    10381 ggggagctgg cccggttctg ggagcagctc cgtcgccggc gctacgacct cgtcctcgat
    10441 tctcaggggc tcgtcaaaag cggcgtggtg gcggcgctgg caagaggaag aaccaaggcc
    10501 gggttctctc acgcctcggc tcgggaaagg gccgccgccc tggcttatcg gcgcggggtt
    10561 cgggtggcgc gcaaccagca tgccatcgac cgccaacgcg agctcttcgc cgcggtgttc
    10621 ggctattgcg tccctgccgg gctcgactac ggaatcgcgc gggcggcgca tcaggccagc
    10681 aagaccaacc attgcgtgct gctgcacgga accacctggg agagcaagca ctacccggag
    10741 catctttggc gggaagtcgc ccggcgcgct cgcgcggccg ggctggacgt agctctgccc
    10801 tggggctccg aggcggagcg tgcgcgggcg gaagcgatcg cgacgacgaa cgccgacgtc
    10861 ctgccggccc tgaaccttgc cgagctgacc acgcgaatcg cctcggctcg gctcgtcgtg
    10921 ggcgtggact cgggactcgc tcacctcagc gcggccctcg acgtgcctac ggtggtgatc
    10981 tacggcagca cggacagcgc actcacggga tgccgcggcc gcagggtgag caatctgcag
    11041 gcgcagtttc cgtgcgcgcc ctgcctgcgc agagcttgcg gctatcacgg cccggaggaa
    11101 attctgcatc aggcgccggt gcggcccgcg tgctacagcc gcgttacgcc cgatgcggtg
    11161 tgggatgccg cagcccgagc catggcggat gggggtcggg agaccgcccc aaacccgggg
    11221 tccgacctgt gacggccacg ggccggccgc tttctttggc ggagctgcag gcgcagggac
    11281 gcgatctgcc gacgccgttc attctggaag gatccctcaa tccggcccct gacgatgggc
    11341 cgatcgagtg tctcgaggtc cttcgcgtgc tcccgggccg gcgaatcgtc atgcgagcgc
    11401 ggctgggggc tcgtcaatgc gcgctcaagc tctttgttgg ggaccgggct gcgctttccg
    11461 ccaggcggga gcgagccggc ctggaactgc tggcggccag cggggtggac acgccggagc
    11521 tgattgccga cctccatctc cccggcggcg gggagggcat cctggtggat tggctggcgg
    11581 gcagccggcc ggtggacgag gaagatgtcc aaggcctggc ggatgttgcg gccgtgcttg
    11641 cacgcctgca cgccaacggt gccatccagc acgacgctca tctgaacaac tacctccgcg
    11701 atgcagacgg catgctgcat gccatcgacg gcgacggcgt tcgccggcct gcacgtcttc
    11761 ggcgcagaca ggaactcgcc aacctcgccc tgctgctggc ccagctgccg ccgccggatg
    11821 cgactcgcct cccggtggtg gcggaagctt actggcgtgc gcgctgggga tcggaccccg
    11881 gcccggacga gctggctgcg ctgcagcgac gggtgcacgc tcagcgagcc ggcaggatgc
    11941 gccgttacct ccggaagacg ctgcgggatt gcagcgagtt tcacggcgag cggaaaaatg
    12001 gccgcttcgt ggtctgtgcc cgaaatgcct ggaccgcgca aatggccgag ttcgtcgacc
    12061 gtccggaaga ccacctggtg gccggcaccg tgctcaaggc aggcaacagc gccacggtga
    12121 tgcgcctgcg aatcggcgaa acggcgtacg tggtcaagcg ctacaacgtc aaaggtttct
    12181 ttcacgggct gcggcgggcg ctgcgcccca cgccgcgcta tcgcgtggcg tggttgagcg
    12241 gtcatcgcct gcttcgcctg ggtat
//
LOCUS       DBNHOX010000060         6750 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_969199, whole genome shotgun sequence.
ACCESSION   DBNHOX010000060 DBNHOX010000000
VERSION     DBNHOX010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_969199"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..963
                     /locus_tag="AC16UZ_01330"
     CDS             163..963
                     /locus_tag="AC16UZ_01330"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6245348.1"
                     /translation="MQGRVALVTGGARGIGRGIAQAFLQAGARVMIGDLGGAGADWKY
                     ELSAESELKRTADELSAMGETHCVAVDVTDAASCARAVDATVQAFGRLDVLVNNAGVV
                     ASGDTESFPEADWDRVFAVNAKGIFLMTRAALPALRRSADAAIINTASIAGKRGFPKM
                     AAYCGSKFAAIGITQSLAAELAPEGIRVNALCPGMVGTAMWLEHLLPANAAKPDEKDD
                     QFKEAMARTIPMGRPQTPQDMGHAAVYLATAANVTGVSLSVAGGYEMN"
     gene            960..1769
                     /locus_tag="AC16UZ_01335"
     CDS             960..1769
                     /locus_tag="AC16UZ_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR6245349.1"
                     /translation="MKKPTAVQNLFREPLLYFLLAGAAIFALAELTDGPGDDYLIEVS
                     EAERARISDQWQAQMGRAPTDEELAGLIEQWLREEIYYREALRMGLDRNDIIIRRRLA
                     QKLTFLTEDVIAGSTPSTEELRSYFEANAERYRIPERITFRHRYFSAERRPNAREDAA
                     AALTDEAVRGDPFMLQKAYALRSSREIADLFGVEFAEGLAQVAPGNWQGPIRSAYGWH
                     VVLVEQRAPGRLPSLEEVQKRAATDLSQERRSRANDEYYHSLRQRYEIIGG"
     gene            1766..2731
                     /locus_tag="AC16UZ_01340"
     CDS             1766..2731
                     /locus_tag="AC16UZ_01340"
                     /inference="COORDINATES: protein motif:HMM:NF025167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HIR6245350.1"
                     /translation="MKALAALLVVLAASQAKAHEVRPAYLQLTETAPGQFEVLWKQPL
                     LGDRRLPLEPVLPEACAREGPPIPEVAGAALIERWRTDCDLRSGAVTISGLSKTLTDV
                     LVEIRYLSAEPVSQLLKPSRPSLDLASPTPQIWSYLTFGVEHLLLGLDHILFVVGLVL
                     FIGNTWTLVKTITGFTIAHSITLACSVLGLISLPQAPVEAVIALSILFLARELVEPPE
                     RRSRITLGRPWLMAFAFGLLHGFGFAGALTEIGLPADQLAVSLLLFNLGIEIGQLLVI
                     GLLLSAAAIFRKAWRRNREVWPRRFAWVMGSVAGFWTIDRVLLVF"
     gene            complement(2764..3171)
                     /locus_tag="AC16UZ_01345"
     CDS             complement(2764..3171)
                     /locus_tag="AC16UZ_01345"
                     /inference="COORDINATES: protein motif:HMM:NF016060.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR6245351.1"
                     /translation="MTDFRTQSRRQVFRYLQVGFAGFVTDVAVLSALVYGMSLGETET
                     GLVGSRFVSFLAAVTVTFLLNARYTFGASIRDSRFPVYVLVQSLGAAITLGTYTALVL
                     HTTLSQAPVVALIIGSAAATTSNYVLVRRFVYV"
     gene            3295..3780
                     /locus_tag="AC16UZ_01350"
     CDS             3295..3780
                     /locus_tag="AC16UZ_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR6245352.1"
                     /translation="MTTAPIECPAQTVEDGWIDYNGHMNMAFYNLAFDRALDHVYDRL
                     GIGADYVRLRGGSCFTREVHVNYLRELSAGDPIRVTFQLLDWDAKRLHFFEEMYHADQ
                     GYLAATSEQLALHVSMTSRSTHPFPQDIQAHLEELMTAHRPLPTPAQVGRVMGIRRSP
                     R"
     gene            complement(3796..5469)
                     /locus_tag="AC16UZ_01355"
     CDS             complement(3796..5469)
                     /locus_tag="AC16UZ_01355"
                     /inference="COORDINATES: protein motif:HMM:NF027688.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin-like phosphoesterase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6245353.1"
                     /translation="MRLCCCVALALLGLGWSSFGLGSGTANGKVFEDLDGDGVWEPGE
                     PGVKGVRVSNGIAVVLTGEDGGYTLPVPEEAVIFITKPSGYATPVNAHQLPQFYYVHQ
                     PDGSPDHLRYPGVDPTGPLPERVDFPLRRQDEPARFQAILFADTQPQTEEELDFIRDD
                     VIAELIGTGARFGMTMGDILFDELSLFPRYNALIGTLGIPWYNVPGNHEMNFLAADDR
                     HALETFKRYFGPPYYAFEYADALFVVLDNIEYLGNGESDPADVRGTGGYVARIGRRQL
                     DWLAAELRHVPVDKLVFLGMHAPLATYLDDSPRNNTADRRELFGLLKGRPNLYAVAGH
                     THTTEHHYFGRDEGFAGPGELHHHVLSTVSGAWWSGPFDTRGVPTSDQRDGTPNGYHV
                     LEVDGTKVTVRYKAASAPADYQMRIMFDVVHHGLRKDAMRDFRHGQLFDGRFSVDQVP
                     AADILVNLFDGGPRSEVAFRIGDGEYLPMRRVRRADPYLIELHARNAETKKSWFKAEP
                     SSHIFVADVPDDLGPGVYPVGVRAIDEFGRAHHAHAVLEITGGSSPSGL"
     gene            complement(5482..6627)
                     /locus_tag="AC16UZ_01360"
     CDS             complement(5482..6627)
                     /locus_tag="AC16UZ_01360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6245354.1"
                     /translation="MPMILNDEQNMLKDSAKDFCSNSAPISQLRRLRDENNADGFDRG
                     TWQAMVDLGWAGIPWPEEHGGLDFGYKGLGVVTEETGRTLAASPLFASVWVCGTLVNL
                     GGSEEQKGELLPLISTGELLMSLALEESHRHDPYGLETRAAAADGGFVLNGRKTFVLD
                     GHVADKLVVAARTSGERGDRNGITLFVVDSDAEGLSVTRTVMVDSRNAANVELDGVRV
                     GADAVLGAVGGAAEVLDPALDIARIGLCAEMLGSLQECFERTVEYLKEREQFGVPIGS
                     FQALKHRAANMFCEVELAKSCVLEALTALDENRDPQEIARLASLAKAKVGETFHLVSR
                     EGIQMHGGIGMTDEFDIGFFIKRAAVTEQTFGDVGFHRNRYGELEGY"
BASE COUNT         1215 a         2268 c         2146 g         1121 t
ORIGIN      
        1 ggttcagacc cacggcggca tgggcatgac cgaggagctg cgcgtcggcc actacttcaa
       61 gcggctgctc gtcatcgacg ctcagttcgg caacgccgac taccatttgc agcgcttcgc
      121 ggcctaagta gaatccggcc tccgcacagg gggaagcacg tcatgcaggg cagagtcgcg
      181 ctggtaaccg gcggcgcccg cggcatcggc cggggcatcg cacaggcctt tctgcaggcc
      241 ggggctcggg tgatgatcgg cgacctcggc ggcgccgggg ccgactggaa gtacgagctg
      301 tctgccgaat cggagctaaa acgcacggca gacgagctgt cggccatggg cgagacccac
      361 tgtgtggccg tcgacgtgac cgacgccgcc agctgcgcgc gcgcggtgga cgccaccgtg
      421 caagccttcg gaaggctcga cgtcctcgtc aacaatgccg gcgtcgtagc ctcgggagac
      481 acggaaagct tccctgaagc cgactgggat cgcgtgttcg cggtgaacgc caaggggatc
      541 tttctcatga cccgggccgc cctgccggcg ctgcggcgat cggccgacgc ggccatcatc
      601 aatacggctt ccatcgcggg aaagcggggc tttccgaaga tggccgctta ctgcggctcc
      661 aagttcgccg ccataggaat cacccagtcc ctggcggccg agctcgcgcc cgagggcatc
      721 cgcgtcaacg ccctgtgtcc cgggatggtg gggaccgcca tgtggctcga gcacctgctt
      781 cctgccaacg cggcgaagcc ggacgaaaaa gacgaccagt tcaaggaggc aatggcgcgc
      841 accattccca tgggccggcc ccagacccct caagacatgg gccacgcagc cgtctaccta
      901 gccacggccg ccaacgtcac cggcgtcagc ctgagcgttg ccggcggcta cgagatgaat
      961 tgaaaaagcc gacggccgtg cagaaccttt tccgcgaacc gctgctttac ttcctgctgg
     1021 caggcgcggc catcttcgcc ctcgccgagc tcacagacgg ccccggcgac gactacctga
     1081 tcgaggtttc cgaagcggag agggcacgga tctcggatca gtggcaggcc cagatgggcc
     1141 gggcgccgac cgacgaggag cttgcgggcc tgatagagca gtggttgcgc gaagagatct
     1201 actatcggga agcccttcga atggggctgg atcgcaacga catcatcatc cggcgccgtc
     1261 ttgcccagaa gctcacgttc ctgaccgagg acgtcattgc cggttccacc ccctccaccg
     1321 aagagcttcg aagctacttc gaagccaacg cggagcggta ccgcatacct gagcgaatca
     1381 ctttccggca ccgctacttc agcgccgagc gccggccgaa tgccagagag gatgccgccg
     1441 ccgccctgac ggacgaggcc gtacgcgggg atcccttcat gctccagaaa gcctacgcgc
     1501 tgcgctcgag ccgggagatc gccgatcttt tcggcgtcga gttcgccgaa gggctggccc
     1561 aggtggcgcc cgggaactgg cagggaccga tccgctcggc ctacggatgg cacgtggtgc
     1621 tcgtggagca gcgggctccc gggcgactgc cgtcgctcga ggaagtgcag aagcgggcgg
     1681 cgacggacct gtcacaggaa cgccgaagcc gagccaacga cgagtactac cactcgctgc
     1741 gtcagcgtta cgagatcatc ggcggatgaa ggcgctggcg gcgctgctcg tcgttctcgc
     1801 tgcgagccag gctaaggccc acgaggtccg cccggcctac ctgcagctga cggaaaccgc
     1861 accgggccag ttcgaagtgc tctggaagca gccgttgctc ggcgaccggc gcctgcccct
     1921 ggaaccggtg ctgcccgagg cgtgcgcgcg cgagggcccg ccgatcccgg aggtcgccgg
     1981 cgctgcgctc atcgaacgct ggcgcaccga ctgcgacctt cgaagcggag ccgtcaccat
     2041 ctccggcctc tccaagacgc tgaccgacgt tctcgtggag attcgctacc tgtcggcaga
     2101 gccagtttcc caattgctca agcccagcag gccatccttg gatctggcgt caccgacccc
     2161 gcagatctgg agctacctca cgttcggcgt ggagcacctg ctgctcgggc tcgaccacat
     2221 cctgttcgtg gtgggcctcg tgctattcat cggcaacacc tggactctgg tgaaaaccat
     2281 cactggcttc accatcgccc acagcatcac gctggcctgc tcggtgctgg ggctgatctc
     2341 gttgcctcag gcccccgtgg aagcggtgat cgcgctgtcg atcctctttc ttgcccggga
     2401 actggtggaa cctcccgagc ggcgctcgcg catcaccctg ggccgcccgt ggctgatggc
     2461 cttcgctttc ggtctgcttc acggcttcgg gtttgccggc gcgctcaccg agatcgggct
     2521 gcccgcagat cagctggccg tctctctgct gctgttcaac ctcggcatcg agatcggcca
     2581 actcctcgtt atcggcctgc tccttagcgc tgcggcgatt ttcaggaagg cctggaggcg
     2641 caaccgcgag gtctggccca ggcggttcgc ctgggtcatg gggtccgtcg cggggttctg
     2701 gaccatcgac cgggtgctgc tcgtcttctg agatcgatct ggctgccgcc gcgaacccga
     2761 tgctcacacg tagacgaagc gtcgcaccag cacgtagttg ctggtggtcg ctgccgccga
     2821 accgatgatc agggcgacca cgggcgcctg ggacaaggtg gtgtggagca ccagcgcggt
     2881 gtaggtgccg agggtgatgg cagcccccag cgactggacc agcacgtaga cggggaacct
     2941 cgagtcccgg atcgaggcgc cgaaggtgta cctggcgttg agaagaaagg tcaccgtcac
     3001 cgcagcgagg aaagaaacaa agcggcttcc cacgagcccc gtctcggtct caccgagaga
     3061 cattccgtag accagcgccg agagcacggc cacgtcggtg acgaagcccg caaagccgac
     3121 ctgaaggtag cggaacacct gtcggcggga ctgcgtgcgg aaatctgtca attcacacct
     3181 cgtccaggca ccttcacccc aagcttacgt ggcagccatg tgcccagcaa ggcgaccgcc
     3241 ttagcctgcc ggcgctttgg ggttaccatc gggaaagact ctggggagac gctcatgacc
     3301 accgcaccca tcgaatgccc ggcacaaacc gtggaggatg gctggatcga ctacaacggc
     3361 cacatgaaca tggccttcta caacctcgcc ttcgaccggg ccttggatca cgtctacgac
     3421 cggctgggca tcggcgcaga ctacgtacgg ctgcgcggcg gctcctgctt caccagggaa
     3481 gtccacgtga actacctgcg cgagctaagc gccggcgacc cgatccgggt gacgtttcag
     3541 ctgctggact gggacgctaa gcggctgcac tttttcgaag agatgtacca cgcagaccag
     3601 ggctaccttg ccgctacctc ggagcagctg gcgcttcacg tgagcatgac aagccgatca
     3661 acgcacccct ttccccagga catccaggcg catctcgagg aactcatgac ggcacatcgg
     3721 ccgcttccga cgccggctca ggtcggacgg gtcatgggca tcagacgcag cccccgctga
     3781 aacggcgcag gaaaatcaga ggcccgacgg cgagctgccg ccggtgattt ccagcaccgc
     3841 atgcgcgtgg tgcgcgcgac cgaactcgtc gatcgcacgc acgcccaccg ggtagacgcc
     3901 cggaccgaga tcgtcgggaa cgtccgcaac gaagatatgc gacgacggct cggccttgaa
     3961 ccaggacttc ttcgtctcgg cgttgcgcgc gtgcagctcg atcagatagg gatccgcgcg
     4021 cctcacccgg cgcatcggca ggtactcccc gtcaccgatg cggaatgcca cctcggagcg
     4081 cggacctcca tcgaagaggt tgaccaggat gtctgcggcg gggacctgat ccacggagaa
     4141 acgaccatcg aacagctgcc cgtggcggaa gtcacgcatg gcgtccttgc gcagaccgtg
     4201 gtgcaccacg tcgaacatga tgcgcatctg gtagtcggcg ggcgcgctgg cagccttgta
     4261 gcgcacggtc accttcgtgc cgtccacctc caggacatgg tacccgttgg gggttccgtc
     4321 gcgctgatcc gacgtgggca cgccgcgggt atcgaacggc ccgctccacc acgccccgga
     4381 aaccgtgctg agcacgtgat ggtggagctc accgggcccg gcaaatcctt cgtccctgcc
     4441 gaagtagtga tgctcggtgg tgtgcgtgtg gccggcgacg gcgtacagat tgggccggcc
     4501 tttgagcaat ccgaagagct cacgccggtc ggcggtattg tttctcgggc tgtcatcgag
     4561 gtaggtggca agcggggcgt gcatgccgag gaaaaccagc ttgtcgacgg gcacgtggcg
     4621 cagctctgcc gccagccagt cgagctgccg cctgccgatc ctggcaacgt agccgccggt
     4681 tccccggacg tcggccggat cgctttcgcc gttaccgaga tactcgatgt tgtccagaac
     4741 cacgaagagc gcatccgcat actcaaaggc gtagtaaggc ggaccgaaat accgcttgaa
     4801 cgtctcaagc gcgtggcgat cgtcagccgc caggaaattc atctcgtgat tgcccggcac
     4861 gttgtaccag ggtatgccca gggtcccgat gagcgcgttg tagcgcggaa aaagcgacag
     4921 ctcgtcgaac aggatgtcac ccatggtcat accgaagcgc gcgccggtgc cgatgagctc
     4981 cgcgatcacg tcgtcccgga tgaaatccag ctcctcctcg gtctggggct gggtgtcggc
     5041 gaacaggatg gcctggaaac gggcgggttc atcctgccgg cgcagcggga aatcgacacg
     5101 ctcgggcaac gggccggtgg gatccacccc ggggtagcgc agatggtcgg gcgagccgtc
     5161 cggctggtgg acgtagtaga actgcggcag ctggtgagcg ttcaccggcg ttgcgtaacc
     5221 gctgggcttg gtgatgaaga tcacggcttc ctcgggaacc ggcagcgtgt agccgccgtc
     5281 ctcgccggtc agcaccacgg cgatgccgtt ggagaccctc acacccttca caccaggctc
     5341 gccgggttcc cagacgccgt cgccgtcaag atcctcgaag acttttccgt tcgccgttcc
     5401 ggagccgagc ccgaagctcg accagcccag accgagcagg gccagcgcga cgcagcagca
     5461 cagcctcaac gtcccgggcc cctagtaacc ttccagctct ccgtatcggt tccggtggaa
     5521 gcccacgtca ccgaaagtct gctcggtcac cgccgctcgc ttgatgaaaa acccgatgtc
     5581 gaactcgtcg gtcatcccga tgccgccatg catctgtatg ccttcccggg aaaccaggtg
     5641 aaacgtctcg cccactttcg ctttcgcgag gctagccagc cgggcgattt cctggggatc
     5701 gcggttctcg tcaagcgccg tcagggcttc gagcacacag gatttggcaa gctcgacctc
     5761 gcagaacatg tttgccgcgc ggtgcttgag ggcctgaaag gaaccgatgg gcacgccgaa
     5821 ctgctcgcgc tccttgagat actcgaccgt gcgctcaaag cactcctgga ggctgcccag
     5881 catctcggcg cagaggccga tgcgggcaat gtcgagcgcc gggtccagaa cctcggccgc
     5941 gccgccgacg gcgccgagca ccgcatcggc gccgacccga acgccgtcca gctcgacgtt
     6001 cgcagcgttg cgcgagtcca ccatgacggt gcgtgtcacc gacagccctt cggcgtcgga
     6061 atccacgaca aacagcgtga tcccgttccg atccccccgc tcacccgaag tccgggcggc
     6121 gaccacgagc ttgtcggcca cgtgcccgtc gagcacgaag gtcttgcgcc cgttcagtac
     6181 gaacccgccg tctgcggcgg cggccctggt ttcgagcccg taagggtcat gacggtgaga
     6241 ctcctcgagc gcgaggctca tcagcagctc accggtggag atcagcggca gcagctcgcc
     6301 cttctgctcc tcgctgccac ccagattcac gagggtgcca cacacccaga cgctggcaaa
     6361 aagcgggctg gccgccaggg tccgaccggt ttcttcggtc accacgccca ggcccttgta
     6421 cccgaagtcg agccccccgt gctcctccgg ccagggaatg cccgcccagc cgagatccac
     6481 catagcctgc caggtgcccc ggtcgaagcc gtctgcgttg ttctcatcgc gcagcctgcg
     6541 cagctgcgaa atgggcgcgc tgttgctgca gaagtccttc gcgctgtcct tgagcatgtt
     6601 ctgctcatcg ttgaggatca tcggcatgat cgcccccgga atcatcgagc ggccaataca
     6661 accgcgtcgc accgacgctg tcaatccgaa ggctgctgac gggtgtaaaa cccctcgaac
     6721 cagggctgcc agacgccggt ttcgcgcgac
//
LOCUS       DBNHOX010000061         8828 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_712338, whole genome shotgun sequence.
ACCESSION   DBNHOX010000061 DBNHOX010000000
VERSION     DBNHOX010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8828)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8828
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_712338"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1556
                     /locus_tag="AC16UZ_01365"
     CDS             <1..1556
                     /locus_tag="AC16UZ_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6245355.1"
                     /translation="EMDRYSQLQQLSRGLSESASDMLDLKDTLLFKARESETLLLQQA
                     RINTELQEGLMRTRMVPFGRLLPRLRRIVRQVSRELGKDVELHAHNMEGELDRNLLER
                     MVPPLEHMLRNAVDHGIESAEERRRAGKPNTGRIDIRLTREGGDVVIEIADDGAGINV
                     ESDRDKAIERGLMAENSVLGDEEICQFVLAPGFSTAKSVTQISGRGVGMDVVHSEVKQ
                     LGGSINIVSRARKGSRFVVRVPFTVSVNRALMVSVGEDLYAIPLNTIEGIVLLSAEEL
                     DKVHPPLSKPFEYAGVRYNVRYLGSYLGREYRGNPSQAAVPVVLVRSGDRAVAVHVDA
                     VQGSREIVVKSLGPQFAGVGGVSGATILGDGSVVVILDLLALIRAQKGDGLTVTRRVM
                     PGAAKTRCVMVVDDSVTVRKVTSRLLERQGMDVLVAKDGIEAIAMLQERRPDVMLLDI
                     EMPRMDGFEVARQVRRDERVQDLPIVMISSRTGEKHQEHAAELGVNRFLGKPFQENEL
                     LATIDSLVN"
     gene            1567..2058
                     /locus_tag="AC16UZ_01370"
     CDS             1567..2058
                     /locus_tag="AC16UZ_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR6245356.1"
                     /translation="MEPATAQELIEMSCVMIPLQETNLLLPNICVAEILPWRRVSSIE
                     DAPKWCLGMLEWRGEVIPVVRFERMGDASVEDAEEGRCLIVMNRSRTIEGLPFYALAA
                     EGLPRIVQLTAEDMANVPTNLGVSETMALRVGSEPAIIPNLQYLEVQVANLVRGQETA
                     KQA"
     gene            complement(2055..2774)
                     /locus_tag="AC16UZ_01375"
     CDS             complement(2055..2774)
                     /locus_tag="AC16UZ_01375"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIR6245357.1"
                     /translation="MSRRIYLEASLLPGATVRLDPDHSHYLTRVMRLKAGDALRCFDG
                     SGREHAARLAEPNPRSCSIEIAEELRNAAPATVRLHLAQAWLKGQAMDLVIQKATELG
                     VTDIWPIEALRSNVRIDAGRTAGRLMHWRGIAKSATEQSERLFLPRVHEPRRLEALLT
                     ERPCQRLILLDPGAAALPGALPFDSLVLMVGPEGGWTEHERELAQAAGADRYGLGDLV
                     LRAETAPIAALAAVRQSWGWR"
     gene            complement(2771..3628)
                     /locus_tag="AC16UZ_01380"
     CDS             complement(2771..3628)
                     /locus_tag="AC16UZ_01380"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR6245358.1"
                     /translation="MPERGQRPRLSFEFFPPRSEEGFASLARVTAKLDELRPDFYSCT
                     YGAGGSTRDGTKQTVARLVSSGHATAPHLSIGADEEETTFAMLDGYRGMGIDRIVALR
                     GDVPSGIGRSRFRANAQALVGRIRKHSGSHFHIEVAAYPEVHPDARSPGSDLEFFRRK
                     VDAGADSAITQYFYSPHAYYDFVDRCAGAGVTIPIYPGIMPITSYEGLIRFSDNCGAD
                     VPRWLRKHLEAFGDDDDGLRRFGIETVSRLCDDLLRAGAPGLHFYTLNRWGASRAICQ
                     NLGLANRDG"
     gene            complement(3654..4808)
                     /gene="metK"
                     /locus_tag="AC16UZ_01385"
     CDS             complement(3654..4808)
                     /gene="metK"
                     /locus_tag="AC16UZ_01385"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007021908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR6245359.1"
                     /translation="MNEYSIFTSESVGEGHPDKMADQISDAVLDAMLEQDAESRVACE
                     TLIKNNLVVLAGEIRTDAWVDVDRLARQVISDIGYGSHDEGFDPWDCTVMNALGVQSS
                     DIAMGVDETSEHEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQKQAEVRRSSLKF
                     LRPDAKSQLTFRYDEAGNPAAIEAIVLSTQHSPDVDQKTIAEAVMEEIIKPVVPSNWI
                     SKDTSIFINPTGQFVIGGPVGDCGLTGRKIIVDTYGGVARHGGGAFSGKDPSKVDRSA
                     AYAGRYVAKNIVAAGLADRCELQVSYAIGVAEPTSISINTFGTGKLDDERIIQLVREH
                     FDLRPKGLIDMLDLKRPIYRNTAAYGHFGRSEPEFTWERTDKAEALAAAL"
     gene            5039..7021
                     /gene="tkt"
                     /locus_tag="AC16UZ_01390"
     CDS             5039..7021
                     /gene="tkt"
                     /locus_tag="AC16UZ_01390"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR6245360.1"
                     /translation="MVTRTDRANAIRALAMDAVQAANSGHPGAPMGLADIAEVLWRDF
                     LKHNPADPAWANRDRFVLSNGHGSMLLYSVLHLSGYAVTMDDLRSFRQLHSRTPGHPE
                     YSECPGVETTTGPLGQGLANAVGMALAEERLSRRFNRDGFSVVDHRTWVIVGDGCLME
                     GISHEAASLAGTLQLGKLICIYDDNGISIDGDVKAWFTEDVPARFEAYGWRVIRDVDG
                     HDPNSIQRALDDAVSRQDRPTLVCCRTVIGFGAPNKQGTADVHGSALGEEEVANARRE
                     LGWAAPPFEIPEPVYASWACREKGAEQQRSWEALLAEYRAAHPEDAAELERRVSGDLP
                     EGWEGPIRALAEASQEAMEPLETRKSSQRCLAALAGALPELFGGSADLTGSNGTRWDG
                     ADEDRYLSFGVREFGMTAITNGMALHGGFRPFSGTFLVFMEYARNAVRLAALMKIPNI
                     FVYTHDSVAVGEDGPTHQPIEQLTNLRTTPGLSTWRPCDTVESAVAWEAAVASRQRPT
                     ALIFTRQKTAPQPRSAQAFADIRRGGYVLKAEAGTLAAVVIATGSEVEIAVAAAASLE
                     SDGVGVRVVSMPSTDVFLSQDRAYQDAVLPPALRARVAVEAAHPDYWYRFVGLDGAVV
                     GIDRFGLSAPGGQAMAALGITADNVAEAVRSVLKEA"
     gene            7029..8192
                     /locus_tag="AC16UZ_01395"
     CDS             7029..8192
                     /locus_tag="AC16UZ_01395"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR6245361.1"
                     /translation="MSQRRNMTDLDLAERRVLIREDLNVPIRDGRITSDARVRAALPT
                     IEAAMRAGARTLVMSHLGRPVEGEFDPALSLAPVAGRLGELLGVDVPLVGLDDVGVID
                     PGAVSLLENVRFLKGEGADDEALARRLASLCDIYVMDAFATAHRAQASTHGVAKFAPV
                     ACAGPLLAGELEALERALRAPARPMTAIVGGAKVSTKLEVLDTLATIADRIIVGGGIA
                     NTFIAAAGHPVGRSLYEEDLLEAARSIAAKVEVPLPVDVMVGERLDAGAVAVLRGLDE
                     VRPDEMSLDIGPETARRYAAELGSAGTILWNGPLGVFEFDQFGEGTRVVAQAIASSGA
                     FSIAGGGDTLAAIDKYDVRDGVSYISTGGGAFLEFVEGKTLPAVAVLASRAEG"
     gene            8206..>8828
                     /locus_tag="AC16UZ_01400"
     CDS             8206..>8828
                     /locus_tag="AC16UZ_01400"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6245362.1"
                     /translation="MEVGFDPDSLRQALKPLVPGSPTVLTPQEDAYLRHYGIHFVEEY
                     PDLAHHFGAVETDSHSVAAHLWSPRDARGTAVIVHGYYDHTGLYGHLIRHLIDRGLAV
                     LSFDLPGHGLSTGAPATIETFDHYVAAFDACIAALGAHLPKPWHLLGQSTGGAVAMEW
                     LLANGVARATSPFSGIVLLAPLVRPYLWPVGRLFYEVVRRFITERERT"
BASE COUNT         1426 a         2827 c         3008 g         1567 t
ORIGIN      
        1 tcgagatgga tcgatactcg cagcttcagc agctctcccg agggctgtcg gagtccgcat
       61 ccgacatgct cgatctgaaa gacaccctgc tgttcaaggc ccgtgagtcc gagactctcc
      121 tgctccagca ggcgcggatc aatacggaac tgcaggaagg gctcatgcgc acgcgcatgg
      181 tgccgttcgg ccgcctgtta ccgcgcctgc gaaggatcgt gcgacaggtt tcccgtgagc
      241 ttggcaagga cgtcgagctg cacgcgcaca acatggaagg cgagctggac cgcaacctcc
      301 tggaacggat ggtgccgccg ctcgagcaca tgttgcgaaa cgcggtggac cacgggatcg
      361 agagcgccga ggaacgacgc cgcgccggca agccgaacac cgggcgtatc gacatccggc
      421 tgacccgcga aggcggcgac gtggtgatcg agatcgccga cgacggggcc ggcatcaacg
      481 tcgagtccga tcgtgacaag gccatcgagc gagggctgat ggcagaaaac tcggtcctgg
      541 gcgacgaaga gatctgccag ttcgttctgg ctcccggatt cagcaccgcc aagtcggtaa
      601 cgcagatatc cggtcgcggc gtgggcatgg acgtggtgca cagcgaggtc aagcaactcg
      661 gcgggagcat caacatcgtc tcgcgggcgc gcaagggctc ccgcttcgtc gttcgcgttc
      721 ccttcaccgt atcggtaaac cgcgcgctca tggtgtcggt gggagaggat ctctacgcca
      781 tcccgctcaa caccatcgaa ggcatagtcc tgctcagcgc cgaggagctg gataaggtgc
      841 atccgcccct ttccaagccc ttcgagtacg ccggcgtccg ctacaacgtg cgatatctcg
      901 gcagctatct tggccgtgaa taccgcggca acccgtctca ggcggcggtg ccggtggtgc
      961 tcgtccggtc tggtgaccgg gcagtggccg tgcatgtgga tgcggtccag ggcagccggg
     1021 aaatcgtagt gaagagcctg ggcccgcagt tcgcgggtgt gggcggtgtg tccggtgcaa
     1081 cgattctcgg ggacggcagc gttgtcgtga tcctcgatct ccttgcgctg attcgcgcgc
     1141 agaagggcga cgggctgacc gtcacccgtc gggtcatgcc tggcgcggcg aagacacgat
     1201 gcgtcatggt ggtggacgac tcggtcacgg ttcgcaaggt gacctcccgg ctgctggagc
     1261 ggcagggcat ggacgtcctc gtggccaagg acggcatcga agccattgcc atgctgcagg
     1321 aacgccgccc ggacgttatg ttgctcgata tcgagatgcc gcgcatggac ggcttcgagg
     1381 tggctcgcca ggtgcggcgc gacgagcgcg tgcaggacct gccgattgtc atgatcagct
     1441 cgcgaacggg cgagaagcac caggagcatg ccgccgagct cggggtgaac cgattcctcg
     1501 gcaagccgtt ccaggagaac gagctgctcg ccaccatcga ctcgctggtg aactaaagag
     1561 aagcccatgg aacctgctac cgcacaagaa ctcatcgaaa tgtcctgcgt gatgatcccg
     1621 ctgcaggaga cgaatctgct gctacccaac atctgcgtag cggagattct gccctggcgc
     1681 cgggtcagca gcatcgagga cgcgccgaag tggtgtctcg ggatgctgga gtggcgtggc
     1741 gaggtcatcc ccgtggttcg tttcgagcga atgggcgatg cgtcggtgga agatgcggaa
     1801 gagggtcgct gcctcatcgt gatgaaccgc tctcgcacca tcgaggggct gccgttttac
     1861 gccctggcgg cggagggtct gccgcgcatc gtgcagctca ccgccgagga catggcgaac
     1921 gtcccaacca atcttggtgt gtccgagacc atggccctca gggttggcag cgagccggcc
     1981 atcatcccca acctgcagta tctcgaggtt caggtggcca acctggtgcg aggccaggaa
     2041 accgccaagc aggcttagcg ccagccccag gactgtcgga ccgcagcgag ggcggcgatg
     2101 ggcgcggttt cggcccggag cacaagatcc cccaagccgt agcgatcggc tcccgccgct
     2161 tgagccagct cccgttcgtg ctcggtccac cccccctcgg gtccgaccat caggaccagc
     2221 gagtcgaaag gcagcgcgcc gggaagagcc gctgcccctg gatcgagcag gatgagccgc
     2281 tggcaggggc gttcggtcaa aagcgcttcg agccgtcggg gctcgtgcac ccgcggcagg
     2341 aacagccgct cgctctgctc cgttgcgctc ttggcgattc cccgccagtg catcaggcgg
     2401 ccagccgtgc gccccgcgtc gattctgacg ttgctgcgca gggcctcgat gggccagatg
     2461 tccgtgaccc cgagctcggt tgctttctga atcacgagat ccatggcctg gcctttcagc
     2521 catgcctggg ccaggtgcag ccggacagtt gccggcgcgg cgttgcgcag ctcctcggcg
     2581 atctcaatcg agcaggagcg ggggttgggt tcggccagcc gggcagcatg ctcgcgcccg
     2641 ctgccgtcaa agcatcgcag agcgtccccg gccttcaggc gcatcacccg cgtgaggtag
     2701 tgggaatggt caggatcgag gcgaacggtt gcaccgggca gcagcgacgc ctccaggtag
     2761 atgcgccgac tcatccgtcc cggttagcca agcccaggtt ctgacagatc gcccggctcg
     2821 cgccccatcg gttgagggta tagaaatgca gaccgggagc gcctgcgcgc agcaggtcgt
     2881 cgcacaggcg gctgacggtt tcgatgccga accggcgcag gccgtcgtcg tcatcgccga
     2941 aggcttccag atgcttgcgc agccagcggg gcacgtccgc gccgcagttg tcggagaaac
     3001 gaatcaggcc ctcgtagctg gtaatgggca tgattcccgg gtagatgggt atggtcaccc
     3061 ccgcccccgc gcagcggtcg acaaagtcgt aataggcgtg cgggctgtag aagtactgcg
     3121 tgatcgcgct gtcggccccg gcgtctactt tgcgccggaa gaattccagg tcggagccgg
     3181 gggatctggc gtcgggatgg acctcgggat aggcggccac ctcgatatgg aagtggctgc
     3241 cggaatgctt ccggatccgg cccaccaggg cttgcgcgtt tgcgcgaaag cgcgagcgtc
     3301 cgatgcccga aggaacatcc ccacgcaacg ccacgatacg gtcgatgccc atgccgcgat
     3361 acccatcgag catggcgaac gtggtctcct cctcgtcggc cccgatggac aggtgcggcg
     3421 cggtggcgtg cccgctgctc acgagccgcg cgaccgtctg cttggtgccg tcccgggtcg
     3481 atccgccggc gccgtacgtg caggaataaa aatctggccg caactcgtcg agtttagccg
     3541 tcacccgggc gaggcttgcg aaaccctcct cgctgcgggg cgggaagaac tcgaagctga
     3601 gccttggccg ctggcctcgt tcgggcatgc tgacgcgtta ctccgggttg acgttacagc
     3661 gctgccgcca gtgcctccgc cttgtcggtg cgctcccagg tgaattcggg ctcagatctg
     3721 ccgaaatgac cgtatgcggc cgtgttccga tagatcgggc gcttcagatc cagcatgtcg
     3781 atgaggcctt tcgggcgcaa atcaaaatgc tcacgtacga gttgaatgat cctctcgtcg
     3841 tcgagcttgc ccgtgccgaa agtgttgatg ctgatggacg tgggctcggc gacgccgatg
     3901 gcgtagctca cctggagctc gcatctgtcg gccagccccg cggcgacgat gttcttggcc
     3961 acgtagcgcc ccgcgtaagc ggcggaacgg tcgaccttgg acgggtcttt gcccgagaag
     4021 gctccgcccc catgccgcgc cacgccgccg taggtgtcga cgatgatctt ccgcccggtc
     4081 agcccgcaat cgccgacggg accgccgatc acgaactgac cggtagggtt gatgaagatg
     4141 ctggtgtctt tcgagatcca gttgctcggc accacgggct tgatgatctc ttccatgacc
     4201 gcctcggcga tggtcttctg gtccacgtct ggcgagtgct gggtggacag cacgatggct
     4261 tcgatggccg cggggttgcc ggcctcgtca tagcgaaacg tgagctggct tttggcgtcg
     4321 ggccgcagaa atttcagcga gctccggcgg acctcggcct gcttctgaac gagccggtgg
     4381 gcgtaggtga tgggggccgg catgagcacg tcggtttcgt tggttgcgta gccgaacatg
     4441 aggccctgat cgccggcacc ctgctcgtgc tcgctggtct cgtccacgcc catggcgatg
     4501 tcggaggact gcaccccgag cgcgttcatc accgtgcagt cccaggggtc gaaaccttcg
     4561 tcgtggctgc cgtaaccgat gtccgagatg acctgacggg ccagccggtc gacgtcgacc
     4621 catgcatcgg tgcggatctc gccggcgaga acgaccagat tgttcttgat cagcgtctcg
     4681 caggcgacgc gggactctgc atcctgttcg agcatggcgt cgagcaccgc atcggagatt
     4741 tgatccgcca tcttgtcggg gtgcccctcg cccaccgact ccgaggtgaa tatgctgtat
     4801 tcgttcatgt ttcttccttc cggttctctt ccaagccctg ccaggccctg ttgcggcaag
     4861 cggcgcattg tacagctttt ctctggttcg actcacggcg cggcctttcc gagcccaggc
     4921 ggccagtgcc cgatggggcg cgcggcattt gcgcatgtcg gtgcgagtaa cggacaatag
     4981 acagggtcgg cagggggctg gcaccgatcc ttactcttac atcgctagcg aaaaccaaat
     5041 ggtaacgagg actgacaggg cgaacgccat tcgtgcgctc gccatggacg cggtgcaggc
     5101 cgctaactcc ggtcatcccg gggcgcccat gggtctggcc gacattgcag aagtgctgtg
     5161 gcgcgacttt ctcaagcaca acccggcgga tccggcctgg gccaaccgcg atcggttcgt
     5221 cctttccaac ggtcacggat ccatgctgct gtacagcgtg ctgcatctgt cggggtatgc
     5281 ggtcaccatg gacgacctgc gctcgttcag gcagcttcac tcgcgcacgc ccggtcaccc
     5341 ggagtacagc gaatgtccgg gtgtcgaaac gaccaccggt ccgctgggcc agggcctggc
     5401 taacgcagtg ggaatggcgc tggccgaaga gcggctctcc cggcgcttca accgcgacgg
     5461 tttctcggtg gtagatcacc ggacctgggt gattgtcggt gacggctgcc tcatggaggg
     5521 gatctctcac gaggcggctt cgcttgccgg caccctgcag ctcggcaagc tcatctgcat
     5581 ctacgacgac aacggcatct ccatcgatgg ggacgtcaaa gcctggttca cggaggacgt
     5641 cccggctcgc tttgaggcct atggttggcg cgtcatacgc gacgtggacg ggcacgaccc
     5701 gaattcgatt cagcgggctc tggacgacgc ggtgagccgg caggatcgac caaccctggt
     5761 ttgctgcagg acggtgatcg gttttggcgc gcccaacaag cagggaactg ccgacgtgca
     5821 cggctcggcg ctgggcgaag aagaggtggc aaacgcccgc cgggagctcg gctgggctgc
     5881 gccgcccttc gagattccgg agcccgtcta cgcctcctgg gcctgtcggg agaaaggcgc
     5941 cgagcagcag cggtcctggg aggcgctgct ggcggagtat cgcgccgcgc acccggagga
     6001 tgcggcggag ctcgaacgcc gcgtgtccgg cgatctgccg gaaggttggg agggtccgat
     6061 ccgcgccctg gccgaagcgt cccaggaggc gatggagccc ctggagacgc ggaaatcgtc
     6121 ccagcgatgc cttgccgcgc tggccggcgc cctgccggag cttttcggag gctccgccga
     6181 tctcacgggc tccaacggga cccgctggga tggtgcagac gaagaccggt atttgagctt
     6241 cggcgttcgc gagttcggca tgacggccat caccaacggc atggccctcc acgggggctt
     6301 tcgaccgttc tccggaactt ttctggtgtt catggagtat gcccgcaacg ccgtgcggct
     6361 tgcggcgctg atgaagatcc ccaacatctt cgtgtacacc catgactccg tggccgtcgg
     6421 tgaggacggc ccgacccatc agcccatcga gcagctcacc aatcttcgga ccacgcccgg
     6481 attgtcgacg tggcgccctt gcgacaccgt ggagagtgcg gttgcctggg aagcggcggt
     6541 ggcgtcccgg cagcgaccga ccgcgctgat attcacccgc cagaagacgg cgccccagcc
     6601 ccgttctgct caggcgttcg ccgacatccg ccggggaggt tatgtcctga aagccgaggc
     6661 cggcacgctg gccgccgtgg tcatcgccac ggggtccgag gtggagatcg ccgtggcggc
     6721 ggcagcgagc ctcgagtcgg acggcgtcgg ggtgcgggtg gtctccatgc cctccaccga
     6781 cgttttcttg tcccaggacc gtgcctacca ggacgccgta ttgccccctg cgctgcgagc
     6841 gcgggtggcg gtggaggccg cgcatcctga ctactggtat cgattcgtgg gcctggacgg
     6901 cgccgtcgtg ggcatcgacc gcttcggtct ctccgcgccg ggtggccagg ccatggccgc
     6961 cctcggcatc accgcggaca acgtcgccga ggccgtccgg agcgttctga aggaggcctg
     7021 atcttttcat gtcgcagcga aggaacatga cggatctgga tctcgcggag cggcgggtgc
     7081 tgatccggga ggatctgaac gtgccgatcc gcgatggccg tatcacgagc gacgcgcggg
     7141 tccgggcggc gctgccgacc atcgaggccg ccatgcgcgc cggcgcgaga accctggtga
     7201 tgtctcacct cggccgcccg gtggagggcg agttcgaccc ggcgttgtcc ttggcccccg
     7261 tggcggggcg gctgggcgag ttgctgggcg tggatgtccc attggtcggc ctggacgacg
     7321 tgggcgtgat cgaccccggc gccgtctcgc tgctggagaa cgttcgcttt ctaaagggcg
     7381 agggtgccga tgacgaagcc ctcgccaggc ggctggcgtc gctgtgcgac atctacgtca
     7441 tggatgcgtt tgccaccgcc catcgcgcac aggcttccac ccacggcgtt gcgaagttcg
     7501 ccccggtggc ctgcgccgga ccgttgctgg ccggtgagct cgaggcgttg gagcgcgcct
     7561 tgagggcgcc cgcccggccc atgacggcca tcgtcggggg tgcgaaggtt tcgaccaagc
     7621 tcgaagtcct ggacaccttg gcgaccattg ccgaccgcat catcgttggc ggcggcatag
     7681 ccaatacctt catcgctgcc gccggtcacc ccgtggggcg ctcgctctac gaggaggacc
     7741 tgctggaagc ggcgcgcagc attgccgcca aggtggaggt gccgctgccg gtggacgtga
     7801 tggtgggcga gcgcctggac gcgggcgcgg tggcggtgct gcgggggctc gacgaggtcc
     7861 gccccgacga gatgagcctg gacatcggtc cggagacggc ccgacgttac gcggcagagc
     7921 ttgggtctgc gggcaccatt ctctggaacg gcccgctggg cgtcttcgag ttcgaccagt
     7981 tcggcgaggg cactcgcgtg gttgcccagg ccatcgcgtc cagcggtgcg ttctccatag
     8041 ccggcggcgg ggataccctg gcagccatcg acaagtacga cgtgcgcgat ggggtgtcct
     8101 acatctccac cgggggaggc gcgttcctcg agttcgtgga gggcaagacc ctgcccgccg
     8161 tggccgtcct cgcgagccgg gctgaaggtt aacggtcagc ggtcgatgga agtcggtttc
     8221 gacccggaca gcctcaggca ggcgcttaag cccctcgtcc ccggctctcc cacggtgctg
     8281 acgccgcagg aggatgctta cctgcggcac tacggcattc acttcgtcga ggagtatccc
     8341 gacctggccc atcatttcgg ggcggtggaa acggattcgc acagcgtcgc cgcccatctc
     8401 tggtcgccgc gcgatgcccg gggcaccgcc gtgatcgtgc acggctacta cgaccacacg
     8461 ggcctttacg ggcacctgat ccgccacctt atcgaccgcg ggcttgccgt gctgtcgttc
     8521 gacctgcccg ggcacgggct atccaccggc gcgccggcca ccatcgaaac gtttgatcac
     8581 tacgtcgccg cgttcgacgc ctgcatcgcg gcgctcggcg cgcacctccc caagccatgg
     8641 cacctgctgg gccagagcac gggaggcgcg gtggccatgg agtggctcct ggccaacggc
     8701 gtcgctcggg cgaccagccc gttttcgggc atcgtgctcc tagcaccgct ggtgcggccg
     8761 tacctgtggc ccgtcggccg gctcttctac gaggtcgtgc ggcgcttcat caccgagcgg
     8821 gagcgaac
//
LOCUS       DBNHOX010000062         2743 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_69333, whole genome shotgun sequence.
ACCESSION   DBNHOX010000062 DBNHOX010000000
VERSION     DBNHOX010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_69333"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="AC16UZ_01405"
     CDS             <1..246
                     /locus_tag="AC16UZ_01405"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR6245363.1"
                     /translation="YQLFQYDQCPFCRRVRQFMDAAGIDIEIRDTLRDPEALRELIRG
                     GGRSMVPCLKIQSEEGTRWLYESADIITYLRERFAAG"
     gene            351..1391
                     /locus_tag="AC16UZ_01410"
     CDS             351..1391
                     /locus_tag="AC16UZ_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6245364.1"
                     /translation="MKLGILSGYSGRKMNIPIDAIRHAESLGYESIWTAEAYGSDAVT
                     PAAWILAQTQKIKVGTAIMQMPARTPAMTAMTAMSLAELSGGRFICGVGASGPQVVEG
                     WHGVPYGLPVTRLKEYVQIMKRIFAREAPLEFEGRMYQLPYQGPGATGLGKPLKSILH
                     CEEDIPIYAATITPKGVQAAAEVADGFFPVWMDPEQYHVFEEPVAKGFASAGGDKDLT
                     RFDIAPFVTVVMGDDVEQCMMPIRGSMALYIGGMGARGKNFYNDYAKRLGFEEAAVRI
                     QDLYLEGRKDEAMAAVPAELIDACHLVGPADRIRERLQRWQAAGRQGHVSSMLLGCQQ
                     PEALALVAETVL"
     gene            complement(1393..2436)
                     /locus_tag="AC16UZ_01415"
     CDS             complement(1393..2436)
                     /locus_tag="AC16UZ_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6245365.1"
                     /translation="MSDVELTPVREMHRFDEDALDRYLQAHVNGYRGPLTVRQFEGGQ
                     SNPTFQLEAGGRLYVLRKQPPGELLPSAHQVDREYRVMKALEQTEVPVPLMKCLCEDA
                     GVIGTKFYVMEMVNGRVFAETLLPSLTPPERRAIYLDLARVLAKLHSVDPAAVGLADF
                     GRPGNYYERQIGRWTKQYEASKTEQIDAMDRLMAWLPERIPEQGETVIVHGDYRLGNA
                     LIHPTQPRIVAVLDWELSTLGDALADLGYICQDYHGDSYNDAGLSGADLGALGIPTED
                     ELVAEYCRHAGREHIDNWPFYVIYNMFRSASIIQGVYKRGLDGNASSEKALEYKDAAR
                     IRSEQAWALVESL"
     gene            2538..>2743
                     /locus_tag="AC16UZ_01420"
     CDS             2538..>2743
                     /locus_tag="AC16UZ_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6245366.1"
                     /translation="MALAKRMAVTLPAGPKIEHTVARLQWAEDNGFADAWFGDSGAPD
                     SLTQVAAVAHHTSSIRIGVAVTPV"
BASE COUNT          461 a          870 c          915 g          497 t
ORIGIN      
        1 tatcagctgt tccagtacga tcagtgcccg ttctgccgtc gggtccgtca gttcatggat
       61 gcggccggca tcgacatcga gatacgcgac acccttcgtg atcccgaagc gctccgcgag
      121 ctgattcgcg gcggcgggcg gtccatggtt ccctgcctga agattcaatc cgaggaaggc
      181 acccgctggc tctacgagag cgcggacatc atcacctacc tgcgggaacg cttcgcagcc
      241 ggctgagcgg tctcggggct cgcgttgccg tgggttgggc ggtctgttaa cttgctgcgc
      301 cttcggcggg cgcggtcccg ccagaaacta cacacctggg ggctcctcat atgaaactcg
      361 gaatcctttc cggctattcc ggacgcaaga tgaacattcc catcgacgcg atcagacacg
      421 ccgagagtct aggctacgaa tccatctgga ccgccgaggc ctacggctcg gacgccgtga
      481 cgccggcggc ctggatcctg gcgcagacgc agaagatcaa ggtgggcacg gccatcatgc
      541 agatgccggc acgcacgcct gccatgacgg cgatgacggc gatgagcctt gccgagctgt
      601 cgggcggtcg tttcatttgc ggcgtcggtg cctccggccc ccaggtcgtg gaaggctggc
      661 acggcgttcc ctacgggctt ccggtgacgc gcctgaagga atacgtgcag atcatgaagc
      721 gcatctttgc ccgggaggcg ccgctcgagt tcgaggggcg gatgtatcag ctgccttatc
      781 agggtccggg tgccacgggt ctgggcaaac ctctgaagag cattcttcac tgcgaggagg
      841 acatccccat ctacgccgcg accataacgc ccaaaggcgt gcaggcagcc gcggaggtgg
      901 cggacggctt cttccccgtg tggatggatc ccgagcagta ccacgtcttc gaagagccgg
      961 ttgccaaggg cttcgccagc gctggcgggg acaaggatct gacccggttc gacatcgcac
     1021 ccttcgtcac cgtggtgatg ggcgacgacg tcgagcagtg catgatgccc atacgcggca
     1081 gcatggcgct ctacatcggc ggcatgggcg cccgcggcaa gaacttctac aacgactacg
     1141 ccaagcggct gggtttcgag gaggctgcgg tcaggatcca ggacctgtac ctcgaagggc
     1201 gcaaggacga agccatggcc gcggtgcccg cggagctcat cgacgcctgt cacctggtgg
     1261 ggccggcgga tcggattcgc gagcggttgc agcgctggca ggcggcgggg cgtcaggggc
     1321 atgtttccag catgctgctg ggttgccagc agccggaggc gctggcgctg gtggcggaaa
     1381 ccgtcctcta gcttagagcg attccaccag ggcccaggcc tgctccgatc ggatgcgggc
     1441 cgcgtccttg tactccagcg ctttctcgga gctggcgttg ccgtccaggc cgcgcttgta
     1501 cacgccctga atgatgctcg ccgagcggaa catgttgtag atcacgtaaa acggccagtt
     1561 gtcgatgtgc tcgcggcccg cgtgccggca gtactcggcc acgagctcgt cctcggtggg
     1621 gatgccgagc gcgcccaggt cggcgccgga aagacccgca tcgttgtagg aatcgccgtg
     1681 gtagtcctgg cagatgtagc cgagatcggc gagcgcgtcc cccagcgtcg acagttccca
     1741 atccagaacc gccacgatgc ggggttgcgt agggtggatc agcgcgttgc ccagccggta
     1801 gtcgccgtgc acgatgacgg tctccccctg ttccgggatg cgttcgggaa gccacgccat
     1861 gagcctgtcc atggcgtcga tctgctcggt cttcgatgcc tcgtactgtt tggtccagcg
     1921 accgatctgc cgctcgtagt agttgccggg gcggccgaag tcggcgagcc ccaccgctgc
     1981 ggggtccacg ctgtggagct ttgccagaac tcgggcgagg tctaagtaga tggcgcgccg
     2041 ttcgggcggg gtcagagacg gaagcagcgt ctctgcgaac acgcgtccgt tcaccatctc
     2101 catcacgtag aacttcgtgc cgatcacgcc tgcgtcctcg cacaggcact tcatcagggg
     2161 aacgggcact tcggtctgct ccagcgcttt catcacccgg tactcgcggt ccacctggtg
     2221 ggccgacggc agcagctcac ccgggggctg cttgcgcaga acgtagagcc tgccacccgc
     2281 ttcgagctga aaggtcgggt tcgactgtcc gccctcgaac tgacgcaccg tcaggggccc
     2341 gcggtagccg ttcacgtggg cctgcaggta acggtccagc gcgtcctcgt cgaatcggtg
     2401 catctcgcgc accggcgtca gctctacatc gctcattgct ttgctcccga agcgacgggc
     2461 aaggatacgg cttgatctcg tcggccgcaa aatgcgaggc tgtgggtttt ccgcaaggcc
     2521 gcacctgggg aggcagcatg gcattggcga agagaatggc ggtcaccttg cccgccggac
     2581 ccaaaatcga gcacaccgtc gcgcgcttgc agtgggccga ggacaacggt ttcgcggatg
     2641 cctggttcgg cgactcgggc gcgccggaca gcctcactca ggtggcggcg gttgcccatc
     2701 acaccagcag catacgcatc ggcgtggccg tcacgccggt gta
//
LOCUS       DBNHOX010000063         4938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1162416, whole genome shotgun sequence.
ACCESSION   DBNHOX010000063 DBNHOX010000000
VERSION     DBNHOX010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1162416"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..974
                     /gene="prmB"
                     /locus_tag="AC16UZ_01425"
     CDS             123..974
                     /gene="prmB"
                     /locus_tag="AC16UZ_01425"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: protein motif:HMM:TIGR03533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR6245367.1"
                     /translation="MTSLVGTIEEVADQFAASDIVYGHGTTNPWDEAVALVLGVTGLP
                     DDQAAADFEIDAESAAEVARLARRRIEDRVPIGYLVGSVNFYGQRFEVQPGIVIPRSP
                     IAQLIEGRLRPWLTRDPRSVLDMCTGSGCLGILCALAFPDAAVTLVDADPAAIALARR
                     NVAAHGLESRVEVVASDLFADLEPARFDLIVSNPPYVDEAEMAALPPEYRHEPAKGLA
                     GGADGLDLVDRLLESLPERLAPGGVFVCEVGASSPALLARYPEIPFLWPDLPDGGEGV
                     FILEGIV"
     gene            1004..2074
                     /gene="aroC"
                     /locus_tag="AC16UZ_01430"
     CDS             1004..2074
                     /gene="aroC"
                     /locus_tag="AC16UZ_01430"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007159018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR6245368.1"
                     /translation="MAGNTFGQLFTVTTFGESHGPALGAVVDGCPPGLALTEADLQAD
                     LDRRAPGRSRYTTQRREPDQVKILSGVFEGVTTGTPIGLVIENVDQRSADYGAIKEVF
                     RPAHADLTYQEKYGVRDYRGGGRASARETAMRVAAGAIAKKYLKERLGLQVRGRLSQI
                     GDVRVGFKSWDVVEDNPFFCADPEAVDAIAALIDGLRREGDSIGAEVTVVASLVPVGL
                     GEPVFGKLDAEIAGALMSINAVKAVGIGDGFDVVGQRGTAHRDEMTAEGFLSNHAGGV
                     LGGISSGQDIIARIALKPTSSLVTPGRSVDKAGNEVEVVTKGRHDPCVGIRATPIAEA
                     MVALVLMDHVMRHRAQNPERGH"
     gene            complement(2034..2954)
                     /locus_tag="AC16UZ_01435"
     CDS             complement(2034..2954)
                     /locus_tag="AC16UZ_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008056358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6245369.1"
                     /translation="MNWNELRAFVSVARHASFSKAAEELHITQPAVTKRVQALEGSLS
                     ARLFDRVGKRVHLTDAGTRLLPRAEHLLREMSDTQRLLRNLKHEVSGVLSLATSHHVG
                     LHRLAPILREFTQRYPDVRLDIRFEDSEAAHDLVREAESELAVVTLDPLGDTELAYEH
                     LWHDPLSFVVAAEHALAGSSKLTLTELARHPVIMPGLGTYTGRIVAEVFSAAGVPLTP
                     AMSTNYLETIGMLVSTGLGWSVLPASMMSADLVSIRTEAPSLHRSLGLVTHPRRTLSN
                     AAQAFTAVALEHRDAISASAPAPDSGLGGA"
     gene            3058..4473
                     /gene="leuC"
                     /locus_tag="AC16UZ_01440"
     CDS             3058..4473
                     /gene="leuC"
                     /locus_tag="AC16UZ_01440"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR6245370.1"
                     /translation="MAAKTLYDKIWDAHVVGVREDGQTMIYIDRHLLHEVTSPQAFEG
                     LALAGRKPWRLDANLATPDHNVPTDRRELGIEGITDLIAREQVETLDANCRRYGITQF
                     DITDRRQGIVHVVGPEQGATLPGMTIVCGDSHTSTHGAFAALAQGIGTSDVEHVLATQ
                     CLVQPKALNFRVAAEGELGVGVYAKDLVLAIIGRIGTAGATGHTIEYQGSAIRSLTME
                     GRMTLCNMSIEAGARAGLVAVDDRTLEYLRERPFAPVGAAWDAAEAYWRGLVTDSQAH
                     FDRDLELNASEIEPQVTWGTSPEMVCGISASTPNPDSVDDPQRSDGLRRALEYMGLGS
                     DQPMTEIAVDRVFIGSCTNSRIEDLRAAARVVKGRRVAGSLKQALVVPGSGLVKAQAE
                     AEGLADVFLAAGFEWRAPGCSMCLAMNPDRLLPGEHCASTSNRNFEGRQGHGGRTHLV
                     SPAMAAAAGIAGRFVDVREYL"
     gene            4470..>4938
                     /gene="leuD"
                     /locus_tag="AC16UZ_01445"
     CDS             4470..>4938
                     /gene="leuD"
                     /locus_tag="AC16UZ_01445"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR6245371.1"
                     /translation="MKAFDKVTGIVVPLDRANVDTDQIIPKQFLKSIHRTGFGDNLFD
                     AWRFEDEGDIGITPNERRINRAFVLNDPRYQGGSILLARANFGCGSSREHAVWALLEY
                     GFRCVIAPSFADIFLSNCFKNGLLPIVLEKEIIEELFVRSAGEEPLELTVDLPG"
BASE COUNT          841 a         1522 c         1667 g          908 t
ORIGIN      
        1 gagctgcttg ccagacccgg ggggccggcg ctcgtggggt tcggggcggg tcctctgctg
       61 ccgcgcgggc ccacgtcgac ccggcgggtc gagctcgtcc ttccgcaccg cctgcatcac
      121 caatgaccag tctggtcggc acgatcgagg aggttgctga tcagtttgcg gccagcgata
      181 tcgtttacgg ccacgggacc acgaatccct gggatgaggc cgtcgctctc gtgctgggag
      241 tgaccggcct tccggatgat caggcagcag ccgatttcga gatcgacgcg gagtcggcgg
      301 cggaggtggc gcgccttgcc cggcgtcgta tcgaggatcg cgtgccgatc ggttacctgg
      361 tgggctccgt gaatttctac gggcaacgct tcgaggtgca gcccggcatc gtcattccgc
      421 gctcgcccat tgcccagctc atcgagggtc gactgcgtcc gtggctgacc cgtgatccgc
      481 gcagcgtcct ggacatgtgc acgggttccg ggtgcctggg catcctgtgc gcccttgcgt
      541 ttcccgacgc ggcggtgacc ctggtggatg cggatccggc tgccatcgcg ctcgcgcgac
      601 gcaacgttgc cgctcacggg ctcgagtctc gggtagaggt agtggcctcc gatctcttcg
      661 ctgatctgga gccggcccgc ttcgacctga tcgtgagcaa tccgccctac gtagacgagg
      721 cggagatggc ggcgcttccc ccggaatacc ggcacgagcc ggcgaagggt ctcgctggcg
      781 gtgcggatgg gctcgacctg gtcgatcggc tgctggagtc gttgccggag cgcctcgctc
      841 cgggcggcgt cttcgtgtgc gaggtgggtg cgagctcgcc ggcgctgctg gcccgctacc
      901 cggaaattcc cttcctctgg cccgatctgc cggatggcgg ggagggtgtg tttattctcg
      961 agggtattgt ctaattcgcc tcgctatcga ggagcggtga ctgatggccg ggaacacgtt
     1021 tggacagctt ttcacggtga cgacctttgg cgaaagccac gggccagcgc tgggggctgt
     1081 ggtggacggt tgccctcccg gcctcgccct gacggaagcc gacctgcagg cggatctcga
     1141 ccgccgcgcc cccggccgtt ccaggtacac gacccagcgt cgggagcccg atcaggtgaa
     1201 gatcctttcc ggcgttttcg aaggcgtcac cacgggcacg cccatcgggc tcgtgatcga
     1261 aaacgtcgac cagcgcagcg ccgactacgg cgcgatcaag gaggtgttcc gtccggccca
     1321 cgcggatttg acctaccagg aaaagtacgg cgttcgcgac tatcggggtg gcggccgggc
     1381 atcggcccgg gagacggcca tgcgcgtcgc ggcgggcgcc atcgcgaaga agtatctcaa
     1441 ggaacggctc gggctgcagg tgcgcggtcg gctctcgcag atcggtgacg ttcgggtcgg
     1501 cttcaaaagc tgggacgtgg tggaggacaa tcctttcttc tgcgctgatc cggaagccgt
     1561 cgatgccatc gccgcgctca tcgatggcct tcgcagggag ggtgattcca tcggtgcgga
     1621 ggtgacggtc gtggcgagct tggtgccggt gggcctcggc gagccggttt tcggtaagct
     1681 cgatgccgag atcgcggggg cgctgatgag catcaacgcg gtcaaggcgg tggggatagg
     1741 cgacggcttc gatgtggttg gccagcgagg cacggcgcat cgcgacgaga tgacggccga
     1801 gggtttcctg agcaaccacg ccggaggcgt gctgggcggt atctcctcgg gtcaggacat
     1861 catcgcgcgg atcgcgctca agccgacgtc cagcctggtc acccccggac ggtcagtgga
     1921 caaagccggc aacgaggtgg aggtggtaac caaagggcgt cacgacccgt gcgtcgggat
     1981 acgcgccacg cccattgcgg aagccatggt tgccctggtg ctgatggacc acgtcatgcg
     2041 ccaccgagcc cagaatccgg agcgggggca ctagcggaaa tcgcatcgcg gtgctccagg
     2101 gcgaccgcgg taaatgcctg agccgcgttg gatagtgtgc gtcgcggatg cgttaccagg
     2161 cccagcgacc gatgcagcga cggcgcctcg gtacggatgg agaccagatc tgcgctcatc
     2221 atgcttgcag gcagcacgct ccagcccagg ccggtgctga ccagcatccc gatggtttcg
     2281 agatagtttg tcgacatggc cggcgtcagg ggcaccccgg ccgctgagaa tacctctgcg
     2341 acgattcgcc ccgtgtacgt tcccaggccg ggcatgatca ccggatggcg cgccaactcg
     2401 gtaagcgtga gcttgctcga gccggccagc gcgtgctcgg cggcgaccac gaagctcagc
     2461 gggtcgtgcc ataaatgctc gtaagccagc tcggtgtcac ccaagggatc cagcgtgacc
     2521 accgccagct cggattccgc ctcccgcacc agatcgtggg cggcttccga gtcctcgaag
     2581 cggatgtcca ggcgaacgtc cgggtagcgc tgggtaaact cgcgcagaat gggggcgagc
     2641 cgatgcagac ccacgtggtg cgacgtggcg aggctcagca cgccggagac ttcgtgcttg
     2701 aggttgcgca ggagccgttg agtatcgctc atctcccgca gcaggtgctc ggccctaggc
     2761 agaagccggg tgccggcgtc tgtgaggtga acgcgcttgc caacgcgatc gaacagcctc
     2821 gctgagaggc tgccctccag ggcctgcacc cgtttcgtga cggcgggctg ggtgatgtgc
     2881 agctcctccg ctgccttgga gaagctcgcg tgccgcgcca cggaaacgaa ggccctcaat
     2941 tcattccagt tcactacaaa tcattccaga ttattatgaa aagtataaaa attatgaatt
     3001 tgttttattc taagccggct tctacgatgt gcaaatctac tttttagccg tagcgacgtg
     3061 gccgcaaaaa ccctttacga caagatatgg gacgctcatg tggtgggcgt gcgcgaagac
     3121 ggtcagacca tgatctacat cgaccgtcac ctgctgcacg aagtgacctc gcctcaggct
     3181 ttcgaaggtc tggctctcgc gggccgtaag ccctggcgat tggacgccaa cctcgcgacc
     3241 ccggatcaca acgtccccac cgacaggcgt gagctgggca tcgagggcat caccgacctc
     3301 atcgccaggg agcaggtcga gaccctggat gcgaactgcc ggcgctacgg catcacccag
     3361 ttcgacatca ccgaccggcg ccagggtatc gtccacgtgg tgggcccgga gcagggcgcc
     3421 accctgccgg gaatgaccat cgtttgcgga gactcccaca cctccaccca tggcgccttt
     3481 gccgcgctgg ctcaggggat cgggacttcc gatgttgagc acgttctggc gacccagtgc
     3541 ctcgtccagc ccaaggccct taatttcagg gtggcagctg aaggggagct tggcgttggc
     3601 gtatatgcca aagacctcgt tctggccatc atcggccgca tcgggaccgc gggagcgacg
     3661 ggacacacca tcgagtacca ggggtccgca atccgctccc tgaccatgga agggcgcatg
     3721 acgctgtgca acatgtccat cgaggcgggt gccagggcgg gcctggtggc cgtggacgat
     3781 cgcacgctgg aatacctgcg cgagcgaccg ttcgcgcccg ttggggcggc ctgggatgcg
     3841 gccgaggcat actggcgagg gctagtaacc gactcgcaag cgcatttcga tcgggatctg
     3901 gagctgaacg cctcggagat cgagccccag gtgacctggg gaacctcgcc cgagatggtg
     3961 tgcggcattt ccgccagcac cccgaatcca gacagcgtgg acgacccgca acgatccgac
     4021 gggttgcgcc gcgcgctcga atacatgggt cttggcagtg atcagcccat gacagagatc
     4081 gccgtcgacc gggttttcat cggctcctgc accaactccc gcatcgagga cctgcgcgcg
     4141 gcggcgcggg tggtgaaggg caggcgggtt gccgggagtc tcaagcaggc gctggtggtg
     4201 cctggttccg gtctcgtcaa agcccaggcc gaagcggaag gtttggccga cgtcttcctg
     4261 gccgccggct tcgagtggcg cgcgccaggg tgctcgatgt gtctcgccat gaatccggat
     4321 cggctgctgc ccggggagca ctgcgcttcc acttcgaatc gcaacttcga ggggcgccag
     4381 gggcacggag gccgcaccca tctggtgagc ccggccatgg ccgccgcggc cggcatcgcc
     4441 ggcagattcg ttgacgtgag ggagtacctg tgaaggcgtt cgataaggta acgggcatcg
     4501 tggttccgct ggaccgggcc aacgtggaca ccgaccagat cattcccaag cagttcctca
     4561 aatccatcca ccggacgggc ttcggggata acctcttcga cgcctggcgt ttcgaagacg
     4621 agggcgacat cggcatcacg ccgaacgagc gccgcatcaa ccgtgctttc gtgctcaacg
     4681 acccccgata tcagggtggc tcaatcctgc tggcccgggc caacttcggc tgcggttcga
     4741 gtcgtgagca cgcggtatgg gcgctgctgg agtacgggtt ccgctgcgtg atcgccccca
     4801 gctttgctga tatttttcta agtaattgct ttaaaaatgg tttacttcct attgttttag
     4861 aaaaagaaat tattgaagag ttgttcgttc gctctgctgg agaagagccg cttgagctca
     4921 ccgtggacct gccgggcc
//
LOCUS       DBNHOX010000064         2231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_262220, whole genome shotgun sequence.
ACCESSION   DBNHOX010000064 DBNHOX010000000
VERSION     DBNHOX010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_262220"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..999
                     /locus_tag="AC16UZ_01450"
     CDS             118..999
                     /locus_tag="AC16UZ_01450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6245372.1"
                     /translation="MSENRFGKRSTAEDVTHDVDLSGKVALVTGVNSGIGTETLRVLA
                     ARGAHVIGTARTLEKAREGCAVSGGATTPLACELTDLDSVRACAVAVADGFEGVDILV
                     ANAGIMALPKLEQVRGIEKQFATNHLGHFLLVTSLLEKLNDGARVVMVSSEGHKGAPK
                     EGIQFDNLSGEKGYSPFRAYGQSKLANILFARSLARRLEGRATANSLHPGVIGTNLGR
                     HMNPVLAAVLGIFFVPFSKSIPQGAATSCLLAANPSVADVTGRYFSDCREVTPSALAR
                     DDELAERLWEVSEELVA"
     gene            1063..1845
                     /locus_tag="AC16UZ_01455"
     CDS             1063..1845
                     /locus_tag="AC16UZ_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6245373.1"
                     /translation="MSEHIIYEVADGVGQLTINRPEKRNAMTFAMLGEFIEAVHRAGE
                     DADARVLIITGVPGAFCAGTDLADLASVPGGERGLRGTAEERGKWWPIVECPKPVIAA
                     IDGPAVGMGAEFTSQCDIRLATPNARFAWNFAHRGLVPDTGAGSWLLPRLIGPAKAMR
                     LLYTGEFLGAEEALEMGYVERVVPPEELAGAARGLAEVIARASPFSHARIKALVYEGL
                     GSEVGPHMQRHTQALAECFKSQDHAEGVAAFLERREPGFTGE"
BASE COUNT          397 a          670 c          771 g          393 t
ORIGIN      
        1 aggcgcaccg gctgggccgc tacgacgatc tgaaaaagcg ctatattgag gaactcgcgg
       61 gcggtccggc cgttcagccc cggagttgaa gcccggcgcg taaattaggg ggaaggcatg
      121 tccgaaaatc ggttcggcaa gagatcgact gcggaggacg tcacgcatga cgtggatctc
      181 agcggcaagg tcgcgctggt gacgggcgtc aattccggca tcggcacgga gacgctgcgg
      241 gtattggcgg cgcgaggcgc ccacgtcatc ggcacggccc gcacgttgga gaaggccagg
      301 gagggctgtg ctgtatcagg cggcgccact acgccgcttg cctgcgagct cacggacctt
      361 gacagcgttc gcgcttgcgc ggtcgccgtg gccgacggct tcgagggcgt ggacatcctt
      421 gttgccaacg ccgggatcat ggcgttgccg aagctcgagc aggtccgcgg tatcgagaag
      481 cagttcgcca ccaaccacct gggccatttc ctgctggtga cgtcgctcct tgagaagctc
      541 aatgacgggg cgcgagtggt gatggtgagc agcgaggggc acaagggcgc gccgaaggag
      601 ggcattcagt tcgacaacct ctccggcgag aagggctaca gcccgttccg cgcctacggc
      661 cagtcgaagc tcgccaatat cctcttcgcg cgcagcctgg cccggcgcct tgaaggccgg
      721 gcaacagcga actcgctgca tcccggcgtg atcggcacga acctggggcg gcacatgaac
      781 cccgttctgg ccgccgtgct tggcatcttc ttcgtgccct tctcgaaatc gatcccccag
      841 ggcgcagcca cctcctgcct gctcgctgcc aacccctccg tggccgacgt cacgggtcgt
      901 tacttctcgg attgccgcga ggtgacgccc agcgcgctgg ctcgggacga tgagttggcg
      961 gagcggctgt gggaagtctc tgaagagttg gtggcctgag gcgttcccgc cgcgcgggca
     1021 ttgttctccc gaccaaccag cgaagcgtaa acggatgcag caatgagtga acacatcatc
     1081 tacgaggtgg ccgacggggt tggtcagctg accatcaacc ggcccgagaa gcgcaacgcc
     1141 atgacgttcg ccatgctcgg cgagttcatt gaagccgtgc atcgcgcggg agaggatgcg
     1201 gacgctcgcg tgctcatcat cacgggggtg cccggagcct tctgcgcggg caccgatctc
     1261 gccgatcttg cgagcgttcc cggtggcgaa cggggtctgc gcggtaccgc ggaggagcgc
     1321 ggaaagtggt ggcccatcgt cgagtgcccg aagcccgtga tcgccgccat cgacggtcct
     1381 gccgtgggca tgggcgcgga gttcacctcc cagtgtgaca ttcgcctggc gacgccgaat
     1441 gcccgctttg cctggaactt cgcccatcgc ggtctcgtgc cggataccgg cgccggaagc
     1501 tggctgctgc cgcgccttat cggcccggcc aaggccatgc gcctgctcta cacgggcgag
     1561 tttctcggcg ctgaggaggc gctggagatg gggtatgtcg agcgcgtggt gccgccggaa
     1621 gagctggcgg gcgcggcgcg cgggctggcg gaggtgattg ccagggcgtc gcccttttcc
     1681 cacgcccgga tcaaggcact cgtctacgag ggcctcggct ccgaggttgg accgcacatg
     1741 cagcgccaca cccaggcgct ggcggaatgt ttcaagagcc aggatcacgc ggaaggcgtg
     1801 gcggccttcc tcgagcggcg tgagcctggg tttacgggcg agtaatgcct cctggctgat
     1861 gccagtgaga gtgccgctgg attgacccga aagcagtcgc gtaacgaaaa aaaccgtcag
     1921 gaatgacgga aaccgtcatt cttgaattcc tcttagcatt caatgaatat acgcggcgga
     1981 gagcgccgtg ccgtcatggt cggcgggttg tttcatggaa ctgatacccc gagctggttc
     2041 agggccgggt cgggaagggg agttaacgtg attagacgat tattgctgag cgccttgggc
     2101 gttctgacca gtattggatt ggtgccgacc gtagtggccg agcaggaacc cggcggagga
     2161 tatctagaag aaatcatcgt cacgggtacc aagcgggctg cagcacagca ggatacgccc
     2221 atcgcgatat c
//
LOCUS       DBNHOX010000065         2790 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_326695, whole genome shotgun sequence.
ACCESSION   DBNHOX010000065 DBNHOX010000000
VERSION     DBNHOX010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_326695"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="AC16UZ_01460"
     CDS             complement(<1..562)
                     /locus_tag="AC16UZ_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245374.1"
                     /translation="MSRIFLLISMLAVAACQPPSDRGGADAPSASVAPAPGAEVGALP
                     SLAERLADRPEADRARDAGRMPAEVVEFLGIEEGMTVLDVFSAGGWYAEVLSHAVGPA
                     GTVYAQNTEFLLSMRDGANEKAMAARLADGRLPNVQRLDRELDDLGLAPGSADAAMLA
                     LNFHDIYNRGGLDGALEFLGRIHAGLK"
     gene            698..1894
                     /locus_tag="AC16UZ_01465"
     CDS             698..1894
                     /locus_tag="AC16UZ_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6245375.1"
                     /translation="MPALDGLRILDMTQYEAGTACTQALAWLGADVVKIEAPGSGDPG
                     RGVWGETSEDSEYFVVWNSNKRSVAIDLRKPEGRDLLLEMLPHYDVFVENYGPGVIER
                     LNLDYDVMRAIHPEIIYARIKGFGLSGPWAEYKCYDMVGQAAASAFSITGEPDGPPMR
                     PGPTTGDAGTGVQMALAITAAYAQKLRTGEGQFIELSMQEAMTYYLRTATASTNYGER
                     PTPRTGNGDLATMSLYPCAGGGPNDYVFLMAVTPRMWGDMCVAIGREDLIEDPRYKRG
                     ADRAANHASLRAEIGRWTLERDKYEAMRILAEGGVPASAVLDTRDLFDNPHLLDRGFV
                     HTVEHEEHGTIRLLGWPARMSRSQVPIKAAPRLGRDTREVIAADLGLAPESVDALVEA
                     GVVAES"
     gene            complement(1896..>2790)
                     /locus_tag="AC16UZ_01470"
     CDS             complement(1896..>2790)
                     /locus_tag="AC16UZ_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6245376.1"
                     /translation="VLCHGFPELAFSWHRVMPRLADAGYRAIAIDQRGYGASDCPPAV
                     EDYAITELAADGAGLLDALGVEKAVYVGHDWGAPVVWNSAVLHPDKVAGVIGVNNPYI
                     PAIPDDPLNLLRAEWGDEHYIVNFHNKGPDDPDSSDAKFNANVEQVFRGLYRASLFSM
                     AEFERLPKEQRVLRMEPIVFNPDPPGRFLLSDEELAYFVAAFEKTGFTPGLNWYRNIS
                     RNWSATRQLDPHIRVPCLMIEVTGDPSGPPGCTELMRPFVEKLDRAVIEDCGHWTQHE
                     KPEELAAIMIAWLDRTFGPEV"
BASE COUNT          485 a          958 c          918 g          429 t
ORIGIN      
        1 gcttgagccc ggcgtggatc cggcccagaa actcgagggc cccgtcgagg ccgccccggt
       61 tatagatgtc gtggaagttc agcgccagca tggccgcatc cgcgctcccg ggcgcgagcc
      121 caaggtcgtc cagctcccgg tcgagccgct gcacgttggg cagccggccg tcggccaacc
      181 gcgccgccat ggctttttcg ttcgcaccgt cgcgcatgct gagcagaaac tcggtgttct
      241 gggcatatac cgtgcctgcc ggcccgacgg cgtgagacag cacctcggca taccagcctc
      301 cggcggagaa cacgtcgagc accgtcatgc cttcctcgat gccgagaaac tcgaccacct
      361 ccgccggcat gcgtccggca tcccgggcgc gatcggcctc cggccggtcg gccagacgct
      421 ccgcgagaga cggtaacgcg ccgacctcgg cgccgggagc gggcgcaacg gacgccgaag
      481 gtgcatctgc gccgccgcga tccgatggcg gctggcacgc tgccacggcc agcattgaaa
      541 tcagcaagaa gatgcggctc atgaactcct ccctctgcct cagtgcgacc cagcatagcg
      601 cgctgcgggc tccccctgta gcccgacggc cgctttcttc atttcggtgc tgaccaccga
      661 tatgcttgtc ggtggggaac acacaagggg aaagcccatg ccggctctgg acggtttgcg
      721 aattctggac atgacccagt acgaggcggg cactgcctgc acccaggcgc tcgcctggct
      781 gggcgccgac gtggtgaaga tcgaggcgcc aggctccggc gaccccggcc gcggcgtctg
      841 gggagaaacc tcggaagatt ccgagtactt cgtggtgtgg aacagcaaca agcgcagcgt
      901 cgccatcgac ctgcgcaaac ccgagggccg cgacctgctg ctcgagatgc tgccccatta
      961 cgacgtgttc gtcgaaaact acggtcccgg cgtgatcgag cgcctgaacc tggattacga
     1021 cgtgatgcgc gccattcacc ccgagatcat ctacgcccgc atcaaggggt tcggcctcag
     1081 cggcccgtgg gcggagtaca agtgctacga catggtgggc caggcggccg ccagcgcgtt
     1141 ttccatcacc ggcgagccgg acggtcctcc gatgcggccg ggccccacca cgggcgacgc
     1201 gggtaccggc gttcagatgg ccctggccat caccgccgcc tacgcgcaga agctgcgcac
     1261 gggagaaggc cagttcatcg agctgtccat gcaggaggcc atgacctact acctgcgcac
     1321 ggccacggcc agcacgaact acggcgagcg cccaaccccg cgaacgggca acggcgatct
     1381 ggcaaccatg tccctgtacc cctgcgccgg cgggggcccc aacgactacg tcttcctgat
     1441 ggcggtgacg ccaaggatgt ggggcgacat gtgcgtagcc ataggccgcg aggatctcat
     1501 cgaggatccc cgctacaaac gcggcgcgga tcgtgcggcc aaccacgcat cgctgcgcgc
     1561 agagatcggc aggtggacgc tcgagcgcga caagtacgag gccatgcgca tcctcgccga
     1621 gggcggcgtg cccgcaagcg ccgtgctcga cacccgggac ctgttcgaca acccccatct
     1681 cctggaccgc ggcttcgtgc acaccgtgga gcacgaggag cacggcacca tccgcctgct
     1741 cggctggccc gcaaggatgt ccaggagcca ggtgcccatc aaagcggcac cgcggctcgg
     1801 cagggacacc cgggaggtca tcgccgccga cctgggcctt gcccccgagt cggtggatgc
     1861 cctggtcgag gccggcgtgg tggcagagtc ctaggctaaa cctccgggcc gaaggtgcgg
     1921 tcgagccagg cgatcattat cgcggccagc tcctcgggtt tctcgtgctg ggtccagtgg
     1981 ccgcagtcct ctatgaccgc gcgatccagc ttttccacga acggccgcat cagctccgtg
     2041 cacccgggcg gtccggaggg gtcgccggtc acctcgatca tcagacacgg cacccggatg
     2101 tgcggatcga gctgccgcgt tgccgaccag ttgcgcgaaa tgttgcggta ccagttcagg
     2161 cccggcgtga agcccgtctt ctcgaacgcg gcgacgaaat aggcgagctc ctcgtcgctt
     2221 aaaagaaacc ggccgggcgg gtcgggattg aagacgatgg gttccatccg gagcacgcgc
     2281 tgctccttgg gcagccgctc gaactccgcc atgctgaaaa ggctcgcccg gtagagaccg
     2341 cgaaacacct gctccacgtt ggcgttgaac ttggcgtcgg aagaatcggg atcgtccggc
     2401 cccttgttgt ggaaattcac gatgtagtgc tcgtcgcccc attccgcccg cagcaggttc
     2461 agcgggtcgt cgggaatggc ggggatgtag gggttgttga ccccgatgac cccggcgacc
     2521 ttgtccgggt gaagcacggc ggagttccag accaccgggg cgccccagtc atggcctacg
     2581 taaacggcct tctcgacgcc gagggcgtcg agcaggccgg ccccgtccgc ggcgagctcc
     2641 gtgatggcgt agtcttccac cgccggcggg cagtcactgg ccccgtaacc gcgctgatcg
     2701 atggcgatgg cgcgatagcc ggcatccgcc agcctcggca tcacccggtg ccatgagaac
     2761 gcaagctccg gaaagccgtg gcagagcacc
//
LOCUS       DBNHOX010000066         7491 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_519607, whole genome shotgun sequence.
ACCESSION   DBNHOX010000066 DBNHOX010000000
VERSION     DBNHOX010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7491)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_519607"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2032
                     /locus_tag="AC16UZ_01475"
     CDS             <1..2032
                     /locus_tag="AC16UZ_01475"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6245377.1"
                     /translation="SPDGRHLAMVRSDDQKDTLVIFARPAMTVATGVASSPGQRFETI
                     TWANNRLVLIEPAADAGAAQQPVPTGAMLALGVDGSRKRLLPRNPEAVRLPTAVISVL
                     PNDLANVLIAAHSACDAESCSEAEKALRRPVKLNLETGEKTFVAPALAMPAYYVSDPS
                     GNRMLAAGRAADGRVEVYRLAAGSWTPVSSFDPAADLGTVPLAVDRAGSVFALANKVG
                     TAGLYRWNLDSGETDEIYRGAGSDIDRVITSYGGRRLLAVRSDPGYASWHYLDPEHPF
                     TRIHTALRAAFPDSDVDVTSFTAEGSEAVVRIYSDRNSGDFYSVSLGTGESTLLATSR
                     PWLPAEDLAKVEPMELTSRDMFTLRGYVTTPASAGPHPMIVWIHDKPTTTRAVWELNA
                     EVQMLARRGFAVLQINYRGSGGLGQWYTASGAERNGQAVQRDIADATRWAIDNGIANE
                     GQICVYGRGYGAFAAVKAMATNIGLFQCAVAIDGFYDLASPFTVPEDPTLTPQNLLTF
                     PSMLDLDDVRRSPVNRVFNIDGSVLLVGESEQTITMRDALEETEKSVVWLAPENEQSG
                     YSEILAYLEAELSAGKAADTDPAGFGATLTARQAREFQRIREEMRAATKELGNRRAFT
                     AAGVRREVEKIIEGYDSDVRSIVTDDQWALYDDFKVGLAQELESELDIVRLQ"
     gene            complement(2106..3677)
                     /locus_tag="AC16UZ_01480"
     CDS             complement(2106..3677)
                     /locus_tag="AC16UZ_01480"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6245378.1"
                     /translation="MAAVVKPGVRTMDPSFAKLLDSDTNAAHVTRAMRRDSPIQPGPT
                     LVPTARYISREYHELEKQRLWSRVWQMAAHEDDFPNVGDVVPYDIVDRSYLVVRVADD
                     EYKAYYNACLHRGRKLRESRARGLDELKCPFHGWAWELDGTLKQVPCAYDYTDLDREA
                     ERLPEVRIGRWGRFIFVNPDPDCEPLEDFLGDLSSQFEFFPYERRYKQAHVAKIIRAN
                     WKVVQEAFMESYHVYMTHPQALAHGVHDGDTKYDVFGNYSRAILCGALDGSGVPDWGP
                     PPEQDEGITRVRHPLTGTIYESAGDGVVNVTTRDGRSGRFDALANWLDGDLIDANPHM
                     CIVVAGVQLPDIGVAGVETTPEDAARAMERLGDDATGRALMSEMQREVLRRVIPSVAD
                     DVPDIELTAAIYLTVFPNYHPWGSFNRINYRFRPNGDNHEECIMECMYLAPIPDDGDY
                     TPVADIHWLGPDDDWTDAPELGSLAGVFNQDVRNMAFVYDGMKASARECVRLADYNET
                     KIRHFHALYGEWVDG"
     gene            3810..5048
                     /locus_tag="AC16UZ_01485"
     CDS             3810..5048
                     /locus_tag="AC16UZ_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6245379.1"
                     /translation="MRTRLPESGSDWPTIKAEMVQLGRGDAKWRDGKTAVYVFNAGDD
                     VAQVQREAYALYMSENGLGPLAFPSLKRMEDEVIDMALSILHAPEDAAGAMTSGGTDS
                     ITMAVKAARNHARAQRGMNAPGNLVIPYSAHPAFDKAAHLMDVQVRRVPVGEDLLADV
                     PAMANAIDRETIMLVGSAPCFPYGLIDDIAALSAVALERGLWLHVDACVGGFIAPFVR
                     MNGSDVPPFDFELPGVASMSADLHKYGYCAKGASTVMFRSDGLYQNMPFDFGDWPGGR
                     MVTPTLAGTRPGGAIAAAWAVMNFLGRTGYQTKHRQVTNARQAVERGVRQLGFEVLGS
                     PKLGIVAFSHAQADVIAVYRHMYRKGWFAGLTTEPKALHLMLSPVHEGVTDLYLTDLA
                     DSLRLAEGQESDDGFEARYS"
     gene            5023..5229
                     /locus_tag="AC16UZ_01490"
     CDS             5023..5229
                     /locus_tag="AC16UZ_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245380.1"
                     /translation="MVSRHDTAELSPSRPFYFWLSLWMWAIAITGFAPGSISGLLDGT
                     FVDSQAVHVHAAVYGAHHDAATID"
     gene            complement(5375..5815)
                     /locus_tag="AC16UZ_01495"
     CDS             complement(5375..5815)
                     /locus_tag="AC16UZ_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6245381.1"
                     /translation="MKVESLGHLVLKVRDLDRSEQFYSGVLGMPVCARFDDKGLKMTF
                     FSLGNHHDLAIMQVSGEEASAGEQAVGLHHVAFCIGRSMDQLREAKAQLEAAGVETDP
                     IDHDVTKSLYFDDPDGHNIELYVDVSDAWKKEPQRIAVLEPLEL"
     gene            complement(5895..6467)
                     /locus_tag="AC16UZ_01500"
     CDS             complement(5895..6467)
                     /locus_tag="AC16UZ_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6245382.1"
                     /translation="MSEAEGLGARVAALEEELGRLTDLQAIQDVLVRYSRALDWLDDA
                     MLETVFYDDADIDYGFFRGNGKDFKKALMELEHGIGRRWHFTSQLEIDLNGDSADVES
                     YNLSLALPEVEPSDGGGYSVFFGFYKDRLVKRGGKWGIIARKHIQVSGAPLAELAMEG
                     DMGLLNQIGLTSPSHPDYRRLTNAQPLDDS"
     gene            complement(6502..7329)
                     /locus_tag="AC16UZ_01505"
     CDS             complement(6502..7329)
                     /locus_tag="AC16UZ_01505"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6245383.1"
                     /translation="MRTVKGSLKIGRFEVPYRRYGDSDHLLMCVSGALQTMAIWRTVV
                     KRFADRYTVVIFDMPGIGRSEIMSGGAHVTVQEQLETVHALIQETHPGGEVTLAGSSW
                     GTAIAAAYAALRPDEVQHLILSSFGMKANAGMEEIVRRALALYEGGDYARGADLILEM
                     FGGQIGETYRRQIIAQFAQLNDAHAEAFAEHCRNIVKLGNLDDIIDLTRIKARTFIIN
                     GANDTIVDLEDMYVAQRLIPDCQVRLIEGVGHFLHFERPELLDDYAEFMTGSAVLTS"
BASE COUNT         1351 a         2458 c         2309 g         1373 t
ORIGIN      
        1 ctcaccggac ggtcggcacc tggcgatggt ccgaagcgac gatcagaagg acactctggt
       61 gatattcgcc cgtccggcca tgaccgtggc caccggcgtc gcatcgagtc ctggtcaacg
      121 cttcgagacc atcacctggg cgaacaaccg cctcgtgctc atcgagcctg cagcagatgc
      181 cggcgcggct cagcaaccgg ttcccacggg cgccatgctc gcgctgggcg tggacggctc
      241 gcgcaagcgc ctgcttcccc gtaacccgga ggccgtcagg cttcccaccg cggtgatcag
      301 cgtgctgccc aacgacctag ccaacgtgct catcgcggcc cacagcgcct gcgacgcgga
      361 gagttgctcc gaggcggaga aagctcttcg caggccggtc aaactcaatc tcgaaacggg
      421 cgaaaagacg tttgtcgccc ctgcccttgc catgcccgcc tactacgtca gcgacccgag
      481 cggcaacagg atgctggctg ccggccgcgc ggcggatggg cgggtcgagg tctatcggct
      541 ggccgcaggc agctggacgc ccgtgtcatc attcgatccc gccgccgatc tcgggacggt
      601 gccgctcgcc gtcgatcggg ccggcagcgt gttcgccctg gccaacaagg tcggcaccgc
      661 cgggctctac agatggaatc tcgattccgg ggagaccgac gagatctatc gcggagccgg
      721 ctccgacatc gaccgggtca tcacgagcta cggtggccgc aggctcctgg ccgtgcgttc
      781 cgacccgggc tacgcaagct ggcactacct ggatccggaa catcccttca ccaggattca
      841 caccgccctg cgcgcggcgt tcccggacag cgacgtcgac gtcaccagct tcaccgcaga
      901 aggttccgag gccgtggtcc gaatctacag cgaccgtaat tcaggcgact tctacagcgt
      961 gagccttggg accggagaga gcacgctgct tgccacgagc cgaccctggc tgccggcgga
     1021 agaccttgcc aaggtcgagc ccatggagct gaccagccgg gacatgttca ctctgcgcgg
     1081 ctacgtcacc actccggcat cggccgggcc tcacccgatg atcgtgtgga tccatgacaa
     1141 gcccaccacc acccgggcgg tctgggagct caacgccgag gtccagatgc tggcgcggcg
     1201 cggtttcgcc gtcctgcaga tcaactaccg gggatcgggc ggtctgggtc agtggtacac
     1261 ggcctcgggc gcagagcgaa acgggcaggc cgttcagcgt gacatcgccg atgccacccg
     1321 ctgggccatc gacaacggca tcgccaacga gggccagatc tgtgtttacg ggcgaggcta
     1381 cggggccttt gccgcagtca aggccatggc caccaacatc ggcctgttcc agtgcgccgt
     1441 cgccatcgac gggttctatg atctggcatc gcccttcacc gtcccggaag accccacgct
     1501 gacgccccag aacctgctga cattcccctc gatgctcgat ctggacgatg tcaggcgctc
     1561 tccggtcaat cgcgttttca acatcgacgg ctcggttctg ctcgtcggcg aatccgaaca
     1621 gacgatcacc atgcgcgatg cgctggaaga gaccgaaaag tccgtggtct ggctggcacc
     1681 cgagaacgag cagagcgggt actcggagat actcgcatat ctcgaagccg agctgagcgc
     1741 gggcaaggcg gcggacacgg atccggccgg cttcggcgcc acgctgaccg cacggcaggc
     1801 cagggagttc cagcggatcc gggaagagat gcgagccgcg accaaggagc tgggaaatcg
     1861 ccgcgcgttc accgccgccg gcgtgcgccg ggaggtggag aagatcatcg aaggctacga
     1921 ctcagacgtc cgatccatcg tcaccgatga tcagtgggcc ctctacgacg acttcaaagt
     1981 cggtctcgcc caggaactgg aaagcgagct ggacatcgtc aggctgcagt gagtcggagc
     2041 tcgcggcccg ttcgcgcccc cattcagcgg gccgacccgg aagccggcat gaccggaaag
     2101 gatcgtcagc cgtccaccca ttccccgtaa agcgcgtgaa agtggcggat cttcgtctcg
     2161 ttgtaatcgg ccagccgcac gcattcccgg gccgatgcct tcatgccgtc gtagacgaag
     2221 gccatgttgc gtacgtcctg attgaaaaca ccggccaggc tgccgagctc gggcgcatcc
     2281 gtccagtcgt cgtcgggacc gagccagtga atgtccgcaa ccggcgtgta gtcaccgtca
     2341 tcgggtatgg gcgcgagata catgcactcc atgatgcatt cctcgtggtt gtccccgttc
     2401 ggtcgaaagc ggtagttgat gcggttgaaa gaaccccacg gatgatagtt cgggaacacg
     2461 gtgagataga tggccgcggt cagctcgatg tcgggaacgt cgtcggctac ggagggtatg
     2521 accctgcgta gcacctcccg ctgcatttcg ctcatgagcg cacgacccgt ggcgtcgtcc
     2581 cccagtcgct ccatggcccg ggccgcatcc tccggcgtgg tctccacgcc tgcaacccca
     2641 atgtccggca gctgcacgcc ggccaccacg atgcacatgt gcggattcgc gtcgatcaga
     2701 tcgccatcga gccagttggc aagcgcatcg aatcggcccg agcggccgtc gcgggtcgtg
     2761 acgttcacca cgccgtcacc ggcggactcg tagatcgtcc ccgtgagcgg atgtcgaacc
     2821 ctggtgatcc cttcgtcctg ctcgggcggc ggcccccaat cgggaacccc tgatccgtcc
     2881 agggctccgc agaggatggc ccgcgaatag ttgccgaaga catcgtattt ggtatcgccg
     2941 tcatgaacac catgagccag cgcctggggg tgcgtcatgt agacgtgata cgactccatg
     3001 aaggcctcct gaacgacctt ccagttcgcg cggatgatct tggcaacgtg ggcctgcttg
     3061 taacgacgct cgtagggaaa gaactcgaac tgcgacgaga ggtcgccgag gaagtcttcc
     3121 aggggctcgc agtccggatc cggattgacg aagatgaagc gcccccagcg tccgatccga
     3181 acctcgggca ggcgctccgc ctcccggtcg aggtcggtgt agtcgtaggc gcagggaacc
     3241 tgcttgagcg tgccgtcgag ctcccaggcc cagccatgaa acgggcattt gagctcatcg
     3301 agccctcttg cccgagactc tctgagcttt cgtccccggt gcaggcaggc gttgtagtag
     3361 gctttgtatt catcgtcggc gacccgaacc accaggtacg acctgtccac gatgtcgtag
     3421 ggcacgacgt cgcccacgtt cgggaaatcg tcctcgtggg cggccatctg ccacacgcgc
     3481 gaccagagac gttgcttctc cagctcgtga tactcacggg atatgtaacg tgcggtgggc
     3541 accagggtag ggcccggctg aatgggcgaa tcgcgccgca tggcacgggt cacgtgagcg
     3601 gcgttggtgt cggagtcgag caacttcgca aacgacgggt ccatcgtccg cacaccgggt
     3661 ttaacgactg ccgccatggc tctcctcccc caatttaccc tgatcgatgc cctaatctac
     3721 cccaaacccg ccgaccggtc tgctgataga tggtaacctc cgggcatcag cggcagcgcc
     3781 gcgccgcgcc gccggacagg ggagcacaga tgagaacgcg cctacctgaa tcgggctccg
     3841 actggccgac aatcaaggcc gagatggttc agctcggccg cggcgatgcg aagtggcggg
     3901 acggcaagac ggccgtttac gtgttcaacg cgggagacga cgtcgcccag gttcaaaggg
     3961 aagcctacgc cctgtacatg tccgagaacg gcctgggacc gctggcattt cccagcctga
     4021 agcggatgga ggacgaggtg atcgacatgg ccctgtcgat cctccacgca cccgaggacg
     4081 ctgcgggcgc gatgacgtca ggcggaaccg attccatcac catggccgtc aaagccgccc
     4141 ggaaccatgc ccgggctcag cgcggcatga acgccccggg caacctggtg attccatact
     4201 cggcccaccc ggctttcgac aaggcggcgc acctcatgga cgtgcaggtg cgcagggtac
     4261 cggtcggaga ggatctcctt gccgacgtgc ccgccatggc gaacgccatc gaccgagaga
     4321 cgatcatgct cgttggctcg gccccctgct tcccctacgg gctgatcgac gacatcgcag
     4381 cgctctctgc cgttgccctc gaacgcggac tatggcttca cgtggatgcc tgcgtgggcg
     4441 ggttcatcgc ccctttcgtg cggatgaacg gctcggacgt tccccctttc gatttcgagc
     4501 tgccaggcgt tgcgtccatg tcggccgacc tgcacaagta cggctactgc gcgaaaggcg
     4561 cgtccaccgt gatgtttcgc agcgacggcc tctaccagaa catgccgttc gacttcggcg
     4621 actggccagg gggtcgcatg gtgacgccga cactggcggg cacacgtccg gggggcgcca
     4681 tcgctgccgc ctgggccgtg atgaacttcc tcggcaggac cggatatcag acaaagcacc
     4741 gccaggtcac caatgcccgg caggccgtcg agcggggggt gcggcagctc ggctttgaag
     4801 tgctcggctc gccgaagctc ggcatcgttg ccttctccca tgctcaggcc gacgtgatcg
     4861 ccgtgtacag acacatgtac cgcaaaggct ggttcgcggg gctgacgacc gagccgaagg
     4921 ctcttcacct catgctgtca cccgtccacg agggggtcac cgatctctac ctgaccgatc
     4981 ttgcagacag cctccggctc gccgaaggcc aggaatccga tgatggtttc gaggcacgat
     5041 acagctgagc tcagcccgtc acggcccttt tacttctggc tcagcttgtg gatgtgggcc
     5101 atagccatca cgggctttgc gccgggatcg atttccggcc tgctggacgg gaccttcgtg
     5161 gattcgcagg ccgtgcacgt tcacgccgcc gtctacggcg cgcaccatga tgctgccacc
     5221 attgattgac atgcttgttt ttcccgtgct cttctgggtc gcctatcgtt ttcggaatcg
     5281 gcccgaaacg cacaagcggc tgatggtcgt cgccacgacg ttgctgctcg tcgcggcagt
     5341 ggggcgcatg agcttccttg gccgcccgcc ggctttacag ttccagcggc tccagcacgg
     5401 cgatgcgctg cggttctttc ttccaggcgt ctgagacgtc cacgtagagc tcgatgttgt
     5461 gcccgtccgg gtcatcgaaa tagagcgact tggtgacatc gtggtctatc gggtccgtct
     5521 cgacgcccgc ggcttcgagc tgcgctttcg cttcacgcag ctgatccatg gacctgccaa
     5581 tgcagaaggc cacgtgatgg agccccactg cctgttcacc ggctgaagct tcttcgccgg
     5641 atacctgcat gatcgccaga tcatggtgat tgcccagact gaagaacgtc atcttcaatc
     5701 ccttgtcatc gaaccgcgca caaaccggca tgccgagcac gccggagtag aattgctccg
     5761 agcggtcgag gtcgcgaact ttgaggacga ggtgcccgag agattcaact ttcatcgtca
     5821 gctccagaac ttcgacatcg caactatgcc ggtcccacgc gtgcaagcca atcacgaatc
     5881 ggcctggaag ccggtcagga atcgtccagc ggctgggcgt tcgtaaggcg ccgatagtcg
     5941 gggtgggagg ggctcgtcag gccgatctgg ttgagcaggc ccatgtcccc ttccatcgcc
     6001 agctccgcca acggggcgcc gctcacctgg atgtgcttgc gcgcgatgat gccccacttg
     6061 cccccgcgct tgacgagccg gtccttgtag aagccgaaga agaccgagta acccccgccg
     6121 tcgctcggtt ccacctccgg aagcgcaagg ctgaggttgt agctctcgac gtcggccgaa
     6181 tcgccgttga ggtcgatctc gagctggctg gtgaaatgcc agcggcgccc tatcccgtgc
     6241 tcgagctcca tcagcgcctt tttgaaatcc tttccgtttc cgcggaaaaa gccgtagtcg
     6301 atgtccgcgt cgtcgtagaa cacggtctcc agcatggcat cgtccagcca gtccagcgcc
     6361 cgggagtagc gcaccagcac gtcctgaatg gcctgcaggt ccgtaagcct cccaagttcc
     6421 tcttcgagcg cagcaacccg cgcacccaac ccttctgctt cactcatggt cttctcctgg
     6481 actgcggatg ctcgacgggt ttcagctcgt caggacggcg gagccggtca tgaactccgc
     6541 atagtcgtca agcagctccg gccgctcgaa atgtagaaag tgccccacgc cctcgatgag
     6601 gcgcacctgg caatccggta tcagtcgctg agccacgtac atgtcctcga gatcgacgat
     6661 tgtgtcgttt gcaccgttga tgatgaacgt tcgcgccttg atccgggtca ggtcgatgat
     6721 gtcgtccaga ttgccgagct tgacaatgtt gcggcaatgc tcggcgaacg cctccgcatg
     6781 ggcgtcgttg agctgagcga actgggcgat gatctgacgt ctgtacgtct cgccgatctg
     6841 ccccccgaac atttccagaa taaggtcggc gccgcgagcg taatctcccc cctcatacag
     6901 cgccagagcg cgcctgacga tttcctccat acccgcgttg gccttcatgc cgaaggagct
     6961 cagaatcagg tgctggacct cgtcgggcct gagcgcggca tacgccgcgg cgatggcggt
     7021 gccccatgag ctcccggcaa gggtcacctc gccgcccgga tgagtttcct gaatgagcgc
     7081 atggacggtt tcgagctgct cctggaccgt gacgtgcgca ccgccggaca tgatctcgct
     7141 gcgcccgatc cccggcatgt cgaagatcac caccgtgtag cgatcggcga aacgtttgac
     7201 cacggttcgc cagatcgcca tggtctgcag cgcgccgctc acgcacatca gcagatggtc
     7261 gctgtcccca taccggcggt aaggcacctc gaagcgtcca atcttaagcg agcctttgac
     7321 tgtcctcatg cttgacctga cgtttccaga tgggcggccg cccgcctgct tgcgatagat
     7381 tgtttccact tgaaccatgc ttgcgggctg agcttgttca tcagccggtc cgtgggcgag
     7441 agaagaagaa ggttaagcgc acccgccgcg cgatctttca gccgcgggct g
//
LOCUS       DBNHOX010000067         1699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1291117, whole genome shotgun sequence.
ACCESSION   DBNHOX010000067 DBNHOX010000000
VERSION     DBNHOX010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1291117"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="AC16UZ_01510"
     CDS             <1..1473
                     /locus_tag="AC16UZ_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR6245384.1"
                     /translation="KSYLSQLSAMSYDERPNVLLVVADDLGRYDLSAYGHTAAHTPNI
                     DALARDGVQFLSAFATAAICAPSRAALLTGRYQNRFGFESQPMQRYVRNLGEYLGFRY
                     LIDTDEMQPFLLDTYPNAEQRLRQGLPASEISLGDALDAAGYATGIFGKWHLGYGPEN
                     HPQAFGFEQQYGFTEAFSLYANEGAPGIVEHRHELFWEEHIWNMGREGPSAITRNGEP
                     IAEARYLTDAIVEETKRFVSDARAEERPFFAYVPFSAPHTPFQARSEDFLAVRGAADH
                     NERVYLAMIQRLDWAVGELVRYLESQGMLDDTLIVFTSDNGGAAYTGATDNGPLRGGK
                     FTHFDGGLAVPLILAQRDVLAPRSVRRPVILTDLFATILRHCGLELPSDRTYDSVDLL
                     TEPDSDTERSLFWRSEFNRTVRRGSWKLVQNRRDGQLQLFNLADDPGEQHDLAEDQPA
                     VVAELVTDLDAWEQEMAPALWPRVMNYHYEDALGSYWFAI"
BASE COUNT          308 a          607 c          512 g          272 t
ORIGIN      
        1 aagagctacc tctcgcagct ctcggcgatg agctacgatg aacgccccaa cgtcctgctg
       61 gtcgtcgccg acgatctggg ccgctacgac ctgtccgcgt acggccacac cgccgcgcac
      121 acccccaaca tcgacgccct ggcgcgggac ggggtgcagt tcctcagcgc cttcgccacc
      181 gccgccatct gcgcgccgtc tcgagcggcg ctgctcacgg gacgctacca gaaccgcttc
      241 ggtttcgagt ctcagcccat gcagcgctac gtgcgcaacc tcggcgagta cctgggattc
      301 cgctacctca tcgacaccga cgagatgcag ccgtttcttc tcgacaccta tcccaacgct
      361 gagcagcgat tgcggcaggg gcttccggcc agcgagatca gccttgggga cgccctcgac
      421 gcggccggct acgccaccgg catctttggc aagtggcatc tgggatacgg cccggaaaac
      481 cacccgcagg ccttcggttt cgagcagcag tacggcttca ccgaagcctt ctcgctttac
      541 gccaacgaag gcgcgcccgg catcgtcgag caccgccacg agctcttctg ggaggagcac
      601 atctggaaca tgggcaggga gggtccgtcc gccatcaccc gcaacggcga gccgattgca
      661 gaggcccgtt acctgaccga cgccatcgtc gaagagacga agcgcttcgt atccgacgcc
      721 agggccgagg aaaggccctt cttcgcctac gtgccgttca gcgcgccgca cacgcctttc
      781 caggcgcgca gcgaggactt tctcgcggtg cggggtgccg cagaccacaa cgagcgcgtt
      841 tacctcgcga tgatccagcg gctcgactgg gccgtgggcg agcttgtccg atacctggaa
      901 tcccagggaa tgctcgacga cacgctcatc gtcttcacca gcgacaacgg cggggcggcg
      961 tataccggcg ccacggacaa cggccctttg cgcggcggca agttcaccca cttcgacggc
     1021 ggtctcgcgg tgcctctcat tctggcccag cgcgacgtcc tggcaccgcg atccgtccgc
     1081 agacccgtca tcctgaccga cctgttcgca accattctcc gtcactgcgg gctggaactc
     1141 ccctcggaca gaacctacga ctctgtggac ctgctgaccg aaccggactc ggacaccgaa
     1201 agatcactgt tctggcgcag cgaattcaac cggaccgtgc gcaggggctc gtggaagctg
     1261 gtccagaacc ggcgagacgg tcagctgcag ctgttcaacc tcgctgacga tcccggcgag
     1321 cagcacgacc tggcggagga ccagcccgcc gtcgttgccg agctcgtcac cgacctcgac
     1381 gcgtgggagc aggagatggc gccggccctg tggccgcgcg taatgaacta tcattacgag
     1441 gacgccctcg ggagctactg gttcgcgatc tgagcccgag gcccccggcg cgcgacttcg
     1501 aacggttgag ggacgagggc catggccacc gctgaaaacg tcgacccgaa gctctggcag
     1561 atcctgcaga agatgaagac cccggatttc gagccgacga caccgctttc gggtccagcc
     1621 ctgtccaaac gacgggtggc catcgtcacc accgccgcca ttcagcgggc tggcgagcgc
     1681 cccttctccg ctcacgcca
//
LOCUS       DBNHOX010000068         2758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_777029, whole genome shotgun sequence.
ACCESSION   DBNHOX010000068 DBNHOX010000000
VERSION     DBNHOX010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_777029"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="AC16UZ_01515"
     CDS             complement(<1..417)
                     /locus_tag="AC16UZ_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6245385.1"
                     /translation="MNITESAVRNPAATLVAALLVLGFGALAIYRLPIQLLPELSPPR
                     IFIYTAWRAAAPQEIEEQIIEPQEKVLKNIQGVTSMVSSISRDQGYIELNFEIGWDIQ
                     QGLIDVITNLQQAPPLPADADEPVVAAGAGTTGNNAA"
     gene            complement(599..1009)
                     /locus_tag="AC16UZ_01520"
     CDS             complement(599..1009)
                     /locus_tag="AC16UZ_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024997.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HIR6245386.1"
                     /translation="MDQGGDVIDMYLQSRRDAEAARRFFRRLLRSHGTSPRKIVTDKL
                     GSHGVARRELMPETIHRNDRHADNRAELSQQPTRATERGMRRFSSPRQAQRFLSAHAV
                     VHSLFNLQRHLASAAFHRLRRARAFKRWNEAVAA"
     gene            complement(1197..2165)
                     /locus_tag="AC16UZ_01525"
     CDS             complement(1197..2165)
                     /locus_tag="AC16UZ_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245387.1"
                     /translation="MNAIIKQVAAMTLLGVAALIHSHDAAAALPTPECTKLYAGKSAE
                     AGTVCVTNDLESLYVTYSTTGDWYLDDVHLFVGATLATMPVTKTGNPKIGNFPYVVEN
                     LNTQMYTFTVPLGDFGAADPCVSPKKIAIAAHAALIRRDTAGVIYQTETGWANGTQLN
                     IKGSWAMYFEYTTRCPPQNSPVTYSCETAFAFGDQTFADLGNIRNARWGWQITVDQGT
                     SGDMTPIYAGAAQNDPSKGSYVGYLTYSYDGATLSLSYTMLDGWVLQATHVYAGDTYT
                     NTTAPGQYGNLHEGLRNVTSDTYQISVTGDPIFVIAHAEACHAVSN"
BASE COUNT          496 a          842 c          902 g          518 t
ORIGIN      
        1 agccgcgttg ttgcccgtgg tgccggcccc ggcggcgacg accggctcgt cggcatccgc
       61 gggcagcggc ggagcctgct gcaggttcgt gatgacgtcg atcagtccct gctgaatgtc
      121 ccagccgatc tcgaaattga gctcgatgta gccctggtcg cgtgagatcg aactgaccat
      181 gctggtcacg ccctggatgt tcttgagtac cttctcctgg ggctcgatga tctgctcttc
      241 gatctcctgg ggcgcggctg cgcgccaggc cgtgtagatg aatatgcgcg gtgggctgag
      301 ctcggggagc agctggatcg gcagtctata gatggcaagc gcgccgaagc cgagcacgag
      361 aagtgcggcg acgagcgtcg ctgccggatt ccgtacggca ctttcggtga tgttcactgg
      421 ctgaccccct gccgaatcag cgtcctgcgc cgacgcggtc cagcgttgat ttagcgccaa
      481 acggccgtcg gaagcaaccg cgggtccgat cgtagcgaag cagcggcgct cgcaatcgac
      541 gcttgacggc attgacaagt tggcggcatc aggcgaggcg aagcttgctc ccgaaggctt
      601 acgccgcgac ggcttcgttc cagcgtttga aggcgcgcgc ccggcgaagc cgatggaatg
      661 ccgccgacgc cagatggcgt tgcaggttga agaggctgtg gacgacagcg tgggcgctca
      721 agaaacgctg cgcctgcctg ggcgagctga agcggcgcat gccacgctcc gtggcccggg
      781 tcggctgctg ggatagttcc gcccgattgt ccgcatggcg atcgttgcgg tggatcgtct
      841 ccggcatcag ttcccggcgg gcgacaccat ggctgcccag cttgtcggtc acgatctttc
      901 gggggctcgt gccatgagac cggagcagcc gccggaagaa tcgcctggcc gcctcggcgt
      961 ccctgcgcga ctgcaggtac atgtcgatga cgtcgccgcc ctgatccacc gcccgcccag
     1021 cggctccagc tcgtctctcg cgacccagac ctgtgcattc agcgactccg cgtgaccgta
     1081 cgaccaccgg gccacgcgcc aggaaagaaa gtccggccat ccaggtctca gggcgaaaaa
     1141 agacagggga cccgaaggtc ccctgtccgg tcagcctgcc tgaaagcctg gtggtttcag
     1201 ttcgaaacag cgtggcaagc ctcggcatgt gcaatcacga agatcgggtc gccagtaacg
     1261 gagatctggt acgtatctga cgtgacgttc ctgaggccct cgtgcaggtt gccgtactgc
     1321 cccggtgcgg tggtattcgt gtaggtgtcg cccgcataca cgtgcgtcgc ctgcagcacc
     1381 cagccgtcca acatcgtgta cgagagggac agcgtcgctc catcgtagct gtacgtgaga
     1441 tagccgacat agctgccctt gctggggtcg ttctgggcgg cgccggcgta gatgggggtc
     1501 atgtcaccag acgtgccctg gtccaccgtg atctgccagc cccagcgcgc gttcctgatg
     1561 ttaccgaggt cggcaaaagt ctggtcgccg aaggcgaagg cggtctcgca ggaatacgtc
     1621 acgggactat tctgcggggg acaccgcgtg gtgtattcga aatacatcgc ccagctgccc
     1681 ttgatattga gctgcgtgcc attcgcccag ccagtctcgg tctggtagat gacgccggcc
     1741 gtgtcgcggc gtatgagcgc tgcgtgggcc gcgatggcga tcttctttgg tgatacgcaa
     1801 ggatcggcgg cgccaaaatc gcccagaggc acggtgaagg tgtacatctg ggtgttgagg
     1861 ttctcgacca cgtacgggaa gttgccaatc ttcgggttgc cggtcttggt caccggcatg
     1921 gtggccaggg tcgcgccgac aaacagatgc acgtcgtcga gataccagtc acccgtggtc
     1981 gaataggtga cgtacagcga ctcgaggtcg ttcgtaacgc aaaccgtgcc ggcctccgcg
     2041 gacttgccgg cataaagctt cgtgcactcc ggcgtgggca gtgccgcagc cgcgtcgtga
     2101 ctgtggatga gggcggccac gcccagcagg gtcatggcag caacttgctt gatgatcgcg
     2161 ttcatgggtc gtcaggttcc tttggttggt gtggccggca agcggcccgg cttgacctcc
     2221 acaaagcacg gttcatgcca acccgcgcct gactcaataa atacgaatta agtcaatatc
     2281 ttgcatctca cgcgaagccc tgcacccagg aaaccgcagc gtccatgcac aagcactaat
     2341 gtgcaaagag cgacgcacaa tgcaacgctt tctgggtaac cgagtgacgc gggacccgaa
     2401 atgacggcga ccgggcgact tgaggatctc gctgtcgcgt cgggcgtctg cctctggccg
     2461 ttaacaggta cctagatcag gctcagcatg gtcccagccg agcgatccgc caatgaccca
     2521 gagcagacat cgtggcccag cgtgagacgg cggttgatga tggggcccag ccgacactcc
     2581 aacgctcact gagcggcatg ttttcggagc ggccaggttt cagcgatgag tcagccgtgt
     2641 cgtccacgtt gcaccttcgc tgtcggcaaa tggcccccgg tggtgctcga acctccaaac
     2701 cacggcttgc cacgatgcgc tggtcccgga ccggggcgga ggctcggtgc aagtcgaa
//
LOCUS       DBNHOX010000069         2455 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_262545, whole genome shotgun sequence.
ACCESSION   DBNHOX010000069 DBNHOX010000000
VERSION     DBNHOX010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_262545"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="AC16UZ_01530"
     CDS             complement(<1..401)
                     /locus_tag="AC16UZ_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007942951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6245388.1"
                     /translation="MEVFDYIIAGAGSAGCVLANRLSEDGARSVLLLEAGGSDRHLFV
                     RMPSAFSLPIGRPRFDWCYQAEAEPALDGRSLACPRGRVLGGSSSINGMVYVRGHPRD
                     FERWESLGATGWGYADVLPYFKRAEHVLGAA"
     gene            534..1634
                     /locus_tag="AC16UZ_01535"
     CDS             534..1634
                     /locus_tag="AC16UZ_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013394.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylethanolamine N-methyltransferase
                     family domain-containing protein"
                     /protein_id="HIR6245389.1"
                     /translation="MSGSLLAGWFAPLAVYLAILILHLALPARRVDGYALDPGTGQPL
                     TYRLNGLVVFALVTSVWLAAGWKGWISWDWLYVNRWSAMGGACLVGLAYSLITVLGAP
                     PTGRSLLADLYLGRPENPQHFNKRVDAKMFLYLAGAVLLGLNTLSATAFHAGALGSAS
                     NPGVYLHAALLTFFVLDYLVFERVHLYTYDLFAERVGLKLGWGCLAFYPFFYPVGLWG
                     TAHLGKPSVIADLGWIWLALSALVFLSGWVLARGANLQKYVFKRFPERAFLGRFPPRV
                     IHGGNLALLCGGFWGVARHVNYLGEILMATGLAMSLGHLDSPWPWLYPLYYLALLVPR
                     ERDDDRRCAEKYGTLWDEYRRRVPYRIVPRIY"
     gene            1658..2152
                     /locus_tag="AC16UZ_01540"
     CDS             1658..2152
                     /locus_tag="AC16UZ_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013981.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HIR6245390.1"
                     /translation="MNLDLILALKVLALALSANGAPVIGKRLFGARWAIPLDGGLTFL
                     DGRPLLGSSKTVRGLVLSLATTTLVATALGYGWQLGVIYSALTMSGDALSSFVKRRRG
                     IPPSGRFRGLDQIPETALPMWVCSSALGLTGWDIAVLVALFFAGDFVLSKVLYRIGIR
                     DEPY"
     gene            complement(2146..>2455)
                     /locus_tag="AC16UZ_01545"
     CDS             complement(2146..>2455)
                     /locus_tag="AC16UZ_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6245391.1"
                     /translation="TVPRILLSHTPEVYRQAARAGVQLLLCGHTHGGQICLPGGYPVT
                     LDSRVPRRLGRGCWDYKGMRGYTSPGAGASMVAVRLNCPPELTVHVFHSAGPGAAQLQ
                     "
BASE COUNT          371 a          826 c          811 g          447 t
ORIGIN      
        1 tcggcagcgc cgagcacatg ctcggcccgc ttgaagtagg gcaggacgtc cgcgtagccc
       61 catcccgtag cacccaggct ctcccagcgc tcgaagtcgc gcgggtggcc gcgcacgtaa
      121 accatgccat tgatggacga cgacccgccc agcacccgcc cccgcgggca ggccaggctt
      181 ctgccgtcga gcgccggctc ggcctccgcc tgatagcacc agtcgaatcg cggccggcca
      241 atgggcagcg agaacgccga aggcatgcga acgaacagat gccgatcgct gccaccggcc
      301 tctaaaagca acaccgatcg ggcaccgtcc tcggagagcc ggttggcgag cacgcatccc
      361 gcggagccgg cccctgcaat gatgtagtcg aagacttcca acgatcccgt accctgccca
      421 gtcactgctg cgcgtctgcc cgaggtgcag gctagcagac gccgggcgga aatggggcac
      481 agcggcgcgg cggcggttag aattccaggt cagcgccccg gggaaggaag atggtgagcg
      541 gtagcctgct ggccggatgg ttcgcgccgc tggcggttta tcttgcgata ctgatcctgc
      601 atctcgccct gcccgcgcgg agggtagacg ggtacgcgct ggatcccggc acgggccagc
      661 ctctgaccta ccgcctgaac ggccttgtcg tgttcgccct cgtcacttcg gtctggctcg
      721 ctgcaggctg gaagggctgg atcagctggg actggctcta cgtcaaccgc tggagcgcaa
      781 tgggcggggc ctgtctggtc gggctggcct acagcctgat caccgtgctc ggcgcgccgc
      841 cgaccggccg gtcactttta gccgatctct acctcggcag gccggaaaat ccgcagcact
      901 tcaacaagcg tgtcgatgcg aagatgtttc tctatctcgc gggcgccgtt ctgctcggcc
      961 tgaacaccct gtccgccacg gcgtttcacg cgggtgccct gggtagcgcc agcaaccccg
     1021 gcgtatatct ccatgcagct ctgctcactt ttttcgtgct ggactatctc gtcttcgagc
     1081 gggttcatct ctacacctac gacctgttcg ccgagcgcgt ggggctcaag ctcggctggg
     1141 gctgcctggc cttctacccc ttcttctacc ctgtgggcct gtggggcacc gcccacctgg
     1201 gcaagccgtc ggtgatcgcc gacctcggct ggatctggct cgcgctgagc gcgctcgttt
     1261 ttctgtccgg ctgggtactg gcgcggggcg caaaccttca gaagtacgtt ttcaaaagat
     1321 tcccggagcg ggcgttcctg ggccgatttc ctccgcgggt cattcacggc ggcaacctgg
     1381 ccctgctgtg cggcgggttc tggggcgtcg cccgacacgt caactacctg ggcgagatcc
     1441 tcatggccac gggtctcgcg atgtccctcg ggcatctgga cagcccctgg ccctggctat
     1501 atcccctgta ctatctggcc ctgctggtgc cgcgggagcg cgatgacgac cgtcgctgtg
     1561 cggagaagta cgggacgctg tgggacgagt accggcgccg ggttccctac cggatcgtcc
     1621 cgcggatcta ttgacgcagc ggagcacgga cctgcacatg aatctggatc tcatacttgc
     1681 cctgaaggtg ctcgcgctgg cgctgagcgc caatggggct ccggtgatcg gcaagcgcct
     1741 gttcggcgcc cgctgggcga tccccctgga cggcggtctc acctttctgg acgggcggcc
     1801 tctgctgggg tcgagcaaaa ccgtccgggg gctggtgctg agcctggcca ccaccaccct
     1861 cgtggcaacg gcgctaggct acggctggca gctgggtgtg atctactcgg cgttgacgat
     1921 gtccggggac gcgctgtcga gcttcgtcaa gcggcgccgg ggcattccgc ccagcggtcg
     1981 gttccgcggc ctggatcaga tccccgaaac cgctctgccc atgtgggtct gcagctcggc
     2041 gctggggctg acgggctggg acatcgcggt gctcgtggcg ttgttcttcg ccggtgattt
     2101 cgtgctgtcg aaagtactct accggatcgg catccgcgac gagccctact gaagctgcgc
     2161 ggcgccaggg cccgccgaat gaaagacgtg caccgtaagc tctggcggac agttcaggcg
     2221 gacggcgacc atggatgccc ctgcgccggg cgacgtgtac ccccgcatgc ctttgtaatc
     2281 ccagcatccg cgtccgaggc gcctgggaac gcgagagtcg agcgtgaccg gatagccccc
     2341 cggcaggcag atctgacccc catgggtatg gccgcacaac agcagctgca cgccggcgcg
     2401 cgccgcctgc cggtagacct cgggcgtatg gctcagcagg atcctgggca ccgtg
//
LOCUS       DBNHOX010000070        12072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_969884, whole genome shotgun sequence.
ACCESSION   DBNHOX010000070 DBNHOX010000000
VERSION     DBNHOX010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12072
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_969884"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="AC16UZ_01550"
     CDS             <1..387
                     /locus_tag="AC16UZ_01550"
                     /inference="COORDINATES: protein motif:HMM:NF023320.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleotide monophosphate
                     nucleosidase domain-containing protein"
                     /protein_id="HIR6245392.1"
                     /translation="TRIGRRVRRVRRHRRQVGDAYYFNWLLNVPLAHQQPFKVTHENV
                     AALRLSADLPAHELVVNLRRAFSAIVTGNVKDDGIRMIESHGPFELHSDRHLIGALEQ
                     LLRSFSENGRMKLIGPYEPCYRVAAG"
     gene            454..1017
                     /gene="ccmA"
                     /locus_tag="AC16UZ_01555"
     CDS             454..1017
                     /gene="ccmA"
                     /locus_tag="AC16UZ_01555"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HIR6245393.1"
                     /translation="MLRVVDAGCRRGGRQLFTELSLELGAGECVELHGPNGSGKSTLL
                     RCIAGLYPDFEGVVETAEFLYAGHRLGLNPLLSAEQNLAWYAALRGTADAVLPALERV
                     GMAGYERTACQHLSAGQQRRVALARLLVCRSPLWLLDEPLTALDGPGQELAVTLVTAH
                     MDGGGAVVAATHQPLGLERSRLLSLGR"
     gene            1041..1712
                     /gene="ccmB"
                     /locus_tag="AC16UZ_01560"
     CDS             1041..1712
                     /gene="ccmB"
                     /locus_tag="AC16UZ_01560"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005740276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR6245394.1"
                     /translation="MAGLFRAQLRRELQTTARSVGVAVNPLVFLFLAMTLFALGAGGG
                     PDDLREHAPGIIWVLVLLASMLSLEALFRSDYDDGTLEQMLLLAEPLFVPLLAKITAQ
                     WCVSGLAMTLLAPLAALILSLPAASVPTLAATLLLGTPALSLLGAVGAALTVGLRRGG
                     VLLGLLILPLYVPVLIFGAGAVSADMAGTGAAGQLYWLAAITMLALTTAPFGVQAALK
                     ISVEQ"
     gene            1837..2580
                     /locus_tag="AC16UZ_01565"
     CDS             1837..2580
                     /locus_tag="AC16UZ_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HIR6245395.1"
                     /translation="MKEFWHRLGSPRWFYGISGPWLKILAGLAALLLGTGCIWGLAFA
                     PPDYQQGNSFRIIYVHVPTAIVAQTAYLVMGCAGVVLLVWRMKLADMVLATVAPFGMW
                     MTVLALMTGAIWGKPTWGAWWVWDARTTSMLVLLFLYFGIYALRQAIPRQEAAGRACA
                     VLAVIGVVNIPIIKYSVDWWLTLHQPATFKLTAAPSMPVEMWLPLAVNVVGIYCLFGA
                     NLLSRLRLEILQREAGTQWVRDMVLEEAR"
     gene            2577..2768
                     /gene="ccmD"
                     /locus_tag="AC16UZ_01570"
     CDS             2577..2768
                     /gene="ccmD"
                     /locus_tag="AC16UZ_01570"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HIR6245396.1"
                     /translation="MSFETFGDFLTMGGHGPYVWLSYGLTLAVVLVNVLAPITGRRRY
                     LREQADAERRRQAAEGVRT"
     gene            2765..3193
                     /gene="ccmE"
                     /locus_tag="AC16UZ_01575"
     CDS             2765..3193
                     /gene="ccmE"
                     /locus_tag="AC16UZ_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017849438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HIR6245397.1"
                     /translation="MNPKRRNRLIVVSFLLLGTMATAFFVLVALNENLNLFYPPDQIV
                     AGEAPLGTQIRAGGMVLEGSVARTGNELDVEFVLTDYQGAAFTVLYSGILPDLFREGQ
                     GILVRGELDPSGVFRAEEVLAKHDENYMPPELDGMHAGPP"
     gene            3190..5145
                     /locus_tag="AC16UZ_01580"
     CDS             3190..5145
                     /locus_tag="AC16UZ_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HIR6245398.1"
                     /translation="MIVELGHLALVIALCLSGLLAVFGLGGLNLAGGRLLRANLSLVT
                     GQAVFCALAFGVLTQAFLADDFSVAYVANNSNTLLPWYYKFSAVWGAHEGSFLLWTLI
                     MSGWTLAVAVLGRTLPLEFHSRVLGVMGFLNLGFLSFLIFTSNPFERLVPLSPAEGSD
                     LNPVLQDFGLIVHPPMLYMGYVGFSVAFAFAVAALLSGRLDAAWARWSRPWSNVAWAF
                     LTVGIALGSWWAYYELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGTFKSWT
                     VLLAITTFSLSLLGAFIVRSGVLTSVHAFAVDPERGLFILGFLILIVGGSLLLYGVRA
                     GVVASRARFEPLSREAFILLNNVLLVVSMAVVLLGTLYPLAYEAVSGGDKISVGVPYF
                     NAGFVPLMLLLAAALATAPLLRWKRTERREILRGLLPALGVSLLLGVGAPLVLAGGVS
                     WQVALSVALACAILATHSRDLWTRARRGVARVPLAYWGMFAAHAGFAVAILGIALTSQ
                     WSVEKDLRMSPGEVVVLDGRAIEFLGVNPHRGSNYVADRGSFSVQEGDARIVLQPEKR
                     RYLAGGNVMTEAAIDAGFTRDLYISLGEPLQGGDWAVRIQVKPFVRWIWLGGLLMAFG
                     GCLAVFDSRYRRLKEKLLSAQVAEAAR"
     gene            5142..5681
                     /locus_tag="AC16UZ_01585"
     CDS             5142..5681
                     /locus_tag="AC16UZ_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HIR6245399.1"
                     /translation="MRRLRLFVPLLVFVIIVGFGYAGFKLNDPRELPSALLDRPFPEF
                     SASLLGGAGTVSRDALLGSPVLVNVWATWCPTCRAEHDELMRIRAETGLRLVGVNYKD
                     DPVKALRWLADFGDPYEFTLQDADGSLGVELGVYGAPESFLLDARGVIVYKRVGEVNE
                     RVWKEEILPALERLHDENG"
     gene            5668..6132
                     /locus_tag="AC16UZ_01590"
     CDS             5668..6132
                     /locus_tag="AC16UZ_01590"
                     /inference="COORDINATES: protein motif:HMM:NF015853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HIR6245400.1"
                     /translation="MRMADRRRAAIFGLLVAAGAALGAGPVDVHDFPDPATEARYRAL
                     IAEFRCPKCLNTNLAGSDAPIAKDLRRTVHRLVVVEGMSDAEVREFLQSRYGDFVLYD
                     PPFRFDTSILWLAPGLLLLVGLGVLYRVLRQPPPPKLSETDARRLREILEES"
     gene            6129..7322
                     /gene="ccmI"
                     /locus_tag="AC16UZ_01595"
     CDS             6129..7322
                     /gene="ccmI"
                     /locus_tag="AC16UZ_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HIR6245401.1"
                     /translation="MSFWLMAGLMMLAGVLIVVVPLLRGGLQGGRTDRSVAVRSLYQE
                     RLAELDVDVAAGQLSPASRDAVAEELGSALLGDFEQTASGQGVDSRPVSPAVVLTLAV
                     LLPLVVLIVYLEVGDPGAQQIAGAEALLAMDPRTQRAEVAGWRDRLDSRVERRSDDAS
                     SWYLLGLANLQLGSFGRAAQAFAMSHALSGESPVVDAYWLQARYLSSGGEIDRETRVI
                     GDRLLKAQPNQPLVLEIYAIDAYRRGDYREAVALFSRALSGNLETEQRAVLAAGLQQA
                     RARLGDLVPSLDVSVTADGASPAGWTLFVIARPPGGGMPYAVVRRPSTSLPLDLRLDD
                     AVAMNADRPLSQAGEVELVVRLSRSGAPAAQAGDWEWRSQLLDLSRLTAPRRVEAVLR
                     EPDPS"
     gene            7428..8111
                     /locus_tag="AC16UZ_01600"
     CDS             7428..8111
                     /locus_tag="AC16UZ_01600"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR6245402.1"
                     /translation="MATRANNNAEQQGCAPAISLTEQIAEHLAREIITGRLGANARIQ
                     ELRVADDLGVSRGSVREALLILESRHLIDLLPRRGAIVRDLTGEELVSFSELFTDLAA
                     AFFGKLARVPDLDLGLPAQAVEEMAMALGRNDLEGSIAARKAFVDGCLAELNDVYLVS
                     VLRALLPSALRLAHRAARHTGYEMRDTIRFHHALLEALSRGDDNRVGELVRAFGRREG
                     QLAAECRSS"
     gene            8197..11697
                     /gene="smc"
                     /locus_tag="AC16UZ_01605"
     CDS             8197..11697
                     /gene="smc"
                     /locus_tag="AC16UZ_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR6245403.1"
                     /translation="MRLKQIKLAGFKSFVDPTTVILPSNRCAVVGPNGCGKSNIIDAV
                     RWVMGESSAKQLRGENLTDVIFNGSNSRKPTAIASIELIFSNEDGRIGGEYAAYAEIS
                     VRRQVTREAQSTYFLNGNKCRRRDIMDIFLGTGFGPRSYSIIEQGMISQLVEAKPEDL
                     RVYLEEAAGISKYKERRRETENRVRHTRENLDRLNDIREELDRQLQHLKRQSRAAERY
                     RELKEEERKLTAELHTIRLVALEAVLDAHRERIQQLEVELSRRQAEQQAIDTQIEQGR
                     QAHADTSAAVNQVQGTFYQLGADLARIEEAIQFNQQRVKQLELDLETVTRRSEETGRQ
                     LAMDETQIRELREQLAELEPRRQEAERGDGLAAARLAELEQKHRAWQAEWDEFRNRAS
                     QNERDAEVQASRVEHLDQLLQRLRGRHDQLLDDAQEVPVLASDDVASLAEEIAGIESR
                     RSGLEADTDRTIKALGSIREDLLVRERMVEEARGELQSLRHDLASLQAVQRAALGRDE
                     RSAQDWVDARGLGSAERLGEALAVVPGWEHAVESVLGDDLQGIRVDDVSPFGVALDHL
                     PGAGVTLVEGSFHAESSGELPSLSSLIRSGSLKLDSLLYGIFAAESVEVAFAHRGGLR
                     AGQSIITRQGIWVGPDWVRILPVSEQDSGIIQRTQELETLGLRVEEAELGLAELQRQV
                     VEGRGQAEELEHDRAKLQAEVNDLNVRLGDMKAQHGVKRVQIEEAGARRERIRREREE
                     LDAQVSEETRKLLEAREALAIAERTREQQDVTREELQARGAASESELAAAREEARANR
                     ERYHALNAEWQSLESRMSASETARGRLVRQRRELDEQRDNLQAGIASSATPLPELRQD
                     LEARLNERLEVERQLGAARAELEQLGERIRGLQAERGEAEAAVETVRSKLETTRVERQ
                     GLAVQQSNLTDQIAATGLGADEVREGLAEDAEEARWVELLERTDRRIQRLGPINLAAI
                     EEFEAQSERKVYLDKQNEDLEQALETLLGAIRRIDRETRARFKETFETVNRHLGELFP
                     KVFGGGHAYLELTGEDLLDTGVSLMARPPGKRNSSVHLLSGGEKAMTAVALIFAIFHL
                     NPSPVCMLDEVDAPLDDVNVMRFAELIKDMSTDVQFVVITHNKLTMEMADHLMGVTMN
                     EPGVSRLVSVDVEEASAMAG"
     gene            11763..>12072
                     /locus_tag="AC16UZ_01610"
     CDS             11763..>12072
                     /locus_tag="AC16UZ_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245404.1"
                     /translation="MDIKDFILIGGGLLIAGVIAHGFYIAWRGRRDTLRMDIAHDLIP
                     EAVDDLHRLRGELPNGGARVVSQASLGEQASLELDTVVVAEPLDAQPPEPAMLAEESA
                     V"
BASE COUNT         1870 a         3781 c         4194 g         2227 t
ORIGIN      
        1 acccggatcg gccgccgggt ccgtcgggtg cgccggcacc ggcgccaggt gggtgacgcc
       61 tactatttca actggttgct caacgttccc ctcgctcacc agcaaccgtt caaggtcacc
      121 catgaaaacg tcgccgccct gcgcctgagc gccgacctgc cggcccatga gctggtggtc
      181 aatctgcggc gcgccttctc ggccatcgtc acgggtaacg tgaaggacga cggcattcgc
      241 atgatcgaaa gccacgggcc gttcgagctg cacagcgacc ggcacctcat cggcgcgctc
      301 gagcagctgc tgcgaagctt ctccgagaac ggccgcatga agctcatcgg cccctacgag
      361 ccctgctacc gggttgccgc cgggtaggtc tggacggtca tcgccttgcc agcgcctttg
      421 gaaccagacc aggttggagc agcagctgcg ccgttgctcc gggtcgtcga cgccggctgc
      481 cgccgaggcg gtcggcagct gttcacggag ctctccctgg aactcggggc tggtgagtgc
      541 gtggagctgc atggccccaa cggcagcgga aagtcgacgc ttctgcgctg cattgccggc
      601 ctgtatccag actttgaagg cgtcgtcgag actgccgagt ttctctatgc gggacatcgg
      661 ctgggtctga acccgctgct gtcggctgag cagaacctgg cttggtacgc cgctttgcgg
      721 ggaaccgcag acgccgtgct gccggcgctg gagcgtgtgg gcatggccgg ttacgagcgt
      781 accgcctgcc agcacctgtc cgccggccag cagcgtcggg tggcgctggc ccggctgctc
      841 gtctgccgct cgccgctgtg gttgctcgac gagccgctga ccgcgctgga cggcccgggg
      901 caggagctcg ccgtgacgct cgtcaccgcg catatggacg gcggcggggc cgtggtggca
      961 gcgacccatc agcccctcgg gctcgagcgt tcgcgcctgc tgagcctggg tcgatgagcg
     1021 gctccgcgaa ggagaacacg gtggccggcc tgttccgggc tcagctgcgg cgcgagctgc
     1081 agacgacggc acgatcggtg ggtgtggcgg tgaacccgct cgtgttcctt ttcctcgcga
     1141 tgacgctctt tgcccttggc gccgggggcg ggcccgacga cctgcgcgag cacgccccgg
     1201 gcatcatctg ggtgctggtg ctgctcgcca gcatgttgtc gctggaagcg ttgtttcgca
     1261 gcgattacga tgacgggacg ctggagcaga tgctgttgct ggccgagccg ctcttcgtgc
     1321 ccctgctggc gaagattacc gcgcagtggt gcgtcagcgg cctggccatg accctgctcg
     1381 ccccgctggc ggcgttgatc ctgtctctgc cggccgccag cgtgccgacg cttgcggcga
     1441 ccctgctgct gggcacgccg gccctgagcc ttttgggcgc cgtgggcgct gcgctcaccg
     1501 tcgggctgcg caggggcggg gtgcttctgg ggcttctgat tttgcctttg tatgtgccgg
     1561 ttttaatatt tggcgccggc gcggtcagcg cggacatggc ggggacgggg gcggcaggtc
     1621 agctctactg gcttgccgcc atcacgatgc tggcgctgac cacggcgccc ttcggcgttc
     1681 aggcagcgct caagatcagc gtggagcagt gacgatgaaa ggcgaacccc ggcccctcct
     1741 gggcgtcaaa ctacgcggat gcatatcgcg gatgcatatg attgctcggc aaacgacaac
     1801 gttacgacgg cgacgtgagg gcggcccttg gcgacgctga aggaattctg gcaccggctg
     1861 ggatccccgc gctggttcta cggcatcagc ggtccctggc tgaagatcct cgcagggctg
     1921 gcggcgctgc tgctgggcac gggatgtatc tgggggctcg ccttcgcccc gcccgactac
     1981 cagcagggca acagtttccg gatcatttac gtccacgtgc ccaccgccat cgtggcccag
     2041 accgcttatc tcgtgatggg gtgcgccggc gtggtcctgc tggtttggcg catgaagctt
     2101 gcagacatgg tgctggcaac ggttgcgccg ttcgggatgt ggatgacggt gctggcgctg
     2161 atgacgggtg cgatctgggg caagcccact tggggcgcct ggtgggtgtg ggatgcgcgc
     2221 acgacttcca tgctggtgct gctctttttg tacttcggga tctacgcgct gcgtcaggcc
     2281 ataccccggc aggaggccgc cgggcgcgcc tgcgccgtgc tggccgtcat cggggtggtg
     2341 aacatcccca tcatcaagta ctccgtcgac tggtggttga ccctgcatca gcctgctacc
     2401 ttcaagctca ccgcggcgcc aagcatgccg gtggagatgt ggctgcctct ggcggtgaac
     2461 gtcgtcggca tctactgcct gttcggtgcc aacctgttga gtcgtcttcg actggagatc
     2521 ctgcagcggg aagcgggcac gcagtgggtg cgcgacatgg tgctcgagga ggcccggtga
     2581 gcttcgaaac gttcggtgac tttctgacga tgggtgggca cggcccgtac gtgtggttga
     2641 gctacgggct gacgctggcc gtggtgctgg tcaacgtgtt ggcgccgatc accggccgcc
     2701 ggcgctacct gcgtgagcag gcggatgcgg aaagacgaag gcaggcggcg gagggtgtgc
     2761 ggacgtgaat ccgaaacggc gtaaccggtt gatcgttgtg tccttcctgc tcctcgggac
     2821 catggcgacg gcgttcttcg tgctggtggc gctcaacgaa aatctgaatc tcttctaccc
     2881 gccggatcag atcgtggccg gcgaagcgcc gctgggcacg cagatccggg caggtggcat
     2941 ggttctcgag ggcagcgtgg cgcgcacggg caatgagctc gacgtggagt tcgtgctcac
     3001 cgattaccag ggcgcagcct tcacggtgct ctacagcggc atcctgcccg atctgttccg
     3061 cgaaggtcag ggaatcctcg tccgcggcga gctcgacccg agcggcgtct tccgggccga
     3121 ggaggtgttg gcgaagcacg acgagaacta catgcctccg gagctggacg gcatgcacgc
     3181 cggcccgccg tgatcgtaga gctgggacat ctggcactgg tcatcgcgct ctgcctgagc
     3241 gggctgcttg ccgtgttcgg cctcggcggt ctgaatctgg cggggggcag gctgctccgg
     3301 gcgaacctgt cgctggtcac cggccaggca gtgttctgcg cgttggcctt cggcgtgctc
     3361 acccaagctt ttcttgccga cgacttttcc gtcgcctacg tggcgaacaa ctccaacacg
     3421 ctgctgccct ggtactacaa gttcagcgcc gtctggggtg ctcacgaagg gtcttttctt
     3481 ctctggacgc tgatcatgtc cggctggacg cttgccgtgg ccgtgctggg acggaccctg
     3541 ccgctggagt ttcacagccg ggtgctgggg gtgatgggct tcctcaatct gggctttctt
     3601 tcgttcctca tcttcacgag caaccccttc gagcggctcg ttccgctctc tcccgcggaa
     3661 ggctctgatc tgaatccggt cctccaggac ttcgggctaa tcgttcaccc gccgatgctc
     3721 tacatgggct acgtgggttt ctccgtggcc ttcgcctttg ccgtggccgc gctgctgtcc
     3781 ggtcggctgg atgccgcctg ggcgcgttgg tcgcgaccct ggagcaacgt ggcatgggct
     3841 tttctgaccg tgggcatcgc gctgggcagc tggtgggcct actacgagct cggctggggt
     3901 ggctggtggt tctgggaccc agtggagaac gcatccttca tgccctggct ggtgggcacg
     3961 gcgctgattc actcccttgc cgtgaccgag aaacgcggaa cgttcaaaag ctggacggtg
     4021 ctcctggcca tcactacctt ttcgctgagc ctgctgggcg cgttcatcgt gcggtccggt
     4081 gttctcacct cggttcacgc cttcgcggtg gatcctgaga gagggctgtt cattctgggc
     4141 ttcctcatcc tcatcgtggg cggctccctg ctgctctacg gcgtgcgggc cggcgtcgtc
     4201 gcgtcccggg cccgtttcga gccgctcagc cgggaagcct tcattctgct caacaacgtg
     4261 ctgctggtgg tcagcatggc ggtggttctg ctgggcacgc tctacccgct ggcctacgag
     4321 gcggtgtcgg gcggagacaa gatttccgtc ggcgtgccgt atttcaatgc gggattcgtc
     4381 cccctcatgc ttctgctcgc agcggcgctg gctaccgcgc ccctgctgcg ctggaagcgc
     4441 acggagcgac gcgagattct gcgcgggctg ttgcccgcgc tcggggtgag cctgctgctc
     4501 ggtgtgggcg cgccgctggt gcttgccgga ggggtatcct ggcaggtcgc cctttccgtg
     4561 gcgttggcct gcgcgatcct ggccacgcac tcacgagacc tctggacccg ggcgcggcgc
     4621 ggcgtcgccc gcgtgccgtt ggcctattgg ggcatgtttg ccgcgcacgc cggcttcgca
     4681 gtggccatcc tgggcatcgc gctgacctcg cagtggtccg tggaaaagga cctgcgcatg
     4741 tccccgggcg aggtcgtcgt gctcgatggt cgggccatcg agtttctcgg cgtcaatccc
     4801 catcgtggtt ccaactacgt ggccgaccgg ggcagcttca gcgtgcagga gggcgatgcc
     4861 cgcatcgtgc tgcagcccga gaagcgtcgc tacctggcgg gcggcaacgt catgaccgag
     4921 gccgccatcg atgctgggtt cacgcgcgac ctttacatct ccctgggcga accccttcaa
     4981 ggaggtgatt gggccgttcg cattcaggtg aagccgttcg tgcgctggat ctggctgggc
     5041 gggcttctga tggctttcgg cggctgcctg gcggttttcg attcgcgcta ccggcggctc
     5101 aaggagaagc tcctgtcggc tcaggtcgcg gaggcggcgc gatgagacgg ctgcgcctgt
     5161 tcgtgccgct gctggtgttc gtcatcatcg tcggtttcgg ctatgcgggc ttcaagctca
     5221 atgatcctcg cgagctcccc tcggcgctgc tcgatcgtcc gtttccagag ttctcggctt
     5281 cgcttctcgg gggagcgggc accgtaagcc gggatgctct gctgggctcc ccggtgctgg
     5341 tcaacgtatg ggcaacctgg tgtcccacct gccgggccga gcacgacgag ctgatgcgca
     5401 ttcgcgccga gacggggctc cgcctcgtgg gggtgaacta caaggacgac ccggtcaagg
     5461 cgctgcgctg gctcgccgat ttcggggacc catacgaatt tacgcttcag gacgcggacg
     5521 gatccctggg ggtggagctc ggcgtctacg gggctccgga aagctttctc ctcgatgcca
     5581 ggggggtgat cgtctacaag cgggtcgggg aggtgaacga gcgcgtctgg aaagaagaaa
     5641 tcctgcccgc gctggagcgc ctgcacgatg agaatggctg atcgtcgtcg cgcggccatt
     5701 ttcggcctgc tcgttgctgc gggtgcggcc ctgggggccg gcccggtgga cgtccacgac
     5761 tttcccgacc cggctacgga agcgcgctat cgtgcgctga ttgcggagtt tcgctgtccg
     5821 aagtgtctga acaccaacct cgccggctcc gatgcgccca ttgccaagga cctgcggcgc
     5881 acggtgcatc ggctcgtcgt cgtcgagggc atgagcgacg ccgaagttcg cgagtttctc
     5941 cagtcgcgtt acggggattt cgtgctttac gacccgccct ttcggttcga cacgtcgatc
     6001 ctctggctgg ctcccggcct cctgttgctc gtaggccttg gggttctgta tcgcgtcctc
     6061 cggcagccgc cgcccccgaa gctcagcgag acagacgccc ggcggctgcg tgagatcctg
     6121 gaagaatcat gagcttctgg ctgatggcgg ggctgatgat gctcgcaggt gtgctcatcg
     6181 tggttgtccc gctgctgcgc ggagggcttc agggcggccg gacggacagg agcgttgcgg
     6241 tccgatccct ctaccaggag cggctcgccg agctggacgt cgacgtggcg gccggtcagc
     6301 tctcgccagc atcccgcgac gcggtggccg aggagctggg ctctgctctg ctcggcgatt
     6361 tcgagcagac ggcgtccggg cagggcgtcg attccagacc ggtgtcgccc gccgtcgtgc
     6421 tgacgctcgc ggtgctgttg cccctggtgg tgctgatcgt ctatctcgag gtcggagatc
     6481 cgggcgcgca gcagatagcg ggcgcggaag cgcttctcgc gatggacccg cgcacccagc
     6541 gcgccgaggt cgccggttgg cgagaccggc tcgatagccg cgttgagcgg cgctcggacg
     6601 atgccagcag ctggtatctc ctgggccttg cgaatcttca gctgggcagc ttcgggcggg
     6661 cggcgcaggc gtttgccatg tcccacgcgc tgtcgggcga aagcccggtg gtcgacgcct
     6721 actggctcca ggctcggtac ctgagctccg ggggcgaaat tgaccgggaa accagggtca
     6781 tcggcgatcg tctgctgaaa gcgcagccca accagccgct ggtgctcgag atttacgcca
     6841 tcgacgccta tcggcgcggc gactatcggg aggcagtggc tctgttctcg cgggctctct
     6901 cgggaaacct ggagaccgaa cagcgcgccg tgctcgcagc cgggctgcag caggcgcgag
     6961 cccgcctcgg agacctggtt ccgagcctgg acgtgtccgt gaccgcggac ggcgcctcgc
     7021 ccgcgggctg gacgctgttc gtcattgccc gcccgccggg aggcgggatg ccgtacgcgg
     7081 tggtccgcag accatcgaca tctctgcccc tggacctgcg gttggacgac gcggtggcga
     7141 tgaacgctga ccggccgctc tcccaggccg gggaagtgga gctggtggtg cggctgagcc
     7201 gctcaggcgc tcccgcggcc caggcgggcg actgggagtg gcgctcccag ttgctggatc
     7261 tttcgcgatt gacggccccg cgcagggtcg aagccgttct gcgggaaccg gatccctcct
     7321 agcgcttcgg ccggcagggg gtgctcgggc cggtttgggc gacggcgaac caatcgaaag
     7381 cggggcggtc atagctctca tccggttccg tacaacgaag gatcgccatg gcgaccagag
     7441 ccaacaacaa tgcggagcaa cagggctgtg ctccggcaat cagcctcacc gaacagatcg
     7501 cggagcatct ggcccgcgag atcatcacag gccgacttgg cgccaatgcc cggatacagg
     7561 aacttcgggt ggcggacgat ctgggagtgt ccagagggtc ggtccgggaa gcgctgctga
     7621 tactggaaag ccgccacctc atcgatctcc tgccccgccg gggcgccatc gtcagggatc
     7681 tcacgggaga ggagctggtc agcttcagcg agctgttcac ggacctggcg gctgcctttt
     7741 tcggaaagct tgcccgcgtt cccgacctcg atctcggcct gccggcccag gcggtcgagg
     7801 aaatggcgat ggccctcggc cggaacgatc tggaaggatc gatcgcggcg aggaaggcgt
     7861 tcgtcgacgg ctgtcttgcc gagctcaacg atgtctacct ggtctccgtg ctgcgtgcgc
     7921 tcctgccatc ggccctgcgt ctggcccatc gggcggcccg acacacgggc tacgagatgc
     7981 gcgatacgat ccgctttcac catgctctcc tcgaagcgct gtcacgcggc gacgacaatc
     8041 gtgtggggga gttggttcgg gcattcggcc ggcgcgaggg tcagctggca gcggagtgcc
     8101 gaagttcctg attttccggg tcgtttgggt gttacgctcg gtaggcaatt gctttagact
     8161 accgcggtct taagacaggg tgatctctcg gtctccatgc ggctaaagca gatcaagctc
     8221 gctggcttca agtctttcgt cgacccgaca accgtcatcc tccccagcaa tcggtgcgcc
     8281 gtcgttggcc ctaacggttg cggcaagtcc aacatcatcg acgcggttcg atgggtcatg
     8341 ggtgaaagtt cggcgaagca gttgcgcggt gaaaacctca ccgacgtcat cttcaacggt
     8401 tccaattcgc gcaagcccac ggcgatcgcc agcatcgagc tgatcttcag caacgaggac
     8461 ggccggatcg gcggcgagta cgcggcatac gccgagatct cggttcggcg ccaggtcacc
     8521 cgggaagccc agtccaccta cttcctcaac ggcaacaaat gccggcgccg ggacatcatg
     8581 gacatcttcc tcggcacggg attcggtccg cgcagctact ccatcatcga gcaggggatg
     8641 atcagccagc tcgtcgaggc gaaacccgaa gacctgcggg tctaccttga ggaagcagcc
     8701 gggatctcga agtacaagga gcgtcgccgg gagaccgaga accgggttcg ccatacccgg
     8761 gaaaacctgg accggctcaa cgacatccgt gaggaactcg acaggcaact tcagcatctc
     8821 aagcgtcaat cccgcgcggc ggagcgatat cgagagctca aagaggaaga gcgcaagctg
     8881 actgccgagc tgcacaccat ccgtctggtg gcgctcgaag ccgtgctcga cgcccatcgg
     8941 gagcggatcc agcagctcga ggtcgagctg agccgccggc aggcggagca gcaggccatc
     9001 gacacgcaga tcgagcaggg tcgccaggct cacgccgata ccagcgccgc cgtcaaccag
     9061 gttcagggga ccttttacca gctcggtgcg gacctcgcgc gcatcgagga ggccattcag
     9121 ttcaaccagc agcgggtcaa gcagctcgag ctcgacctgg agacggttac ccggcgctcg
     9181 gaggaaacgg gccgtcagct ggccatggac gagacgcaga ttcgcgagct gcgtgagcag
     9241 ctcgccgagc tggagccgcg ccggcaggag gccgagcgcg gggatggcct ggccgccgcc
     9301 cgcctggccg agctcgagca gaagcaccgg gcgtggcagg ctgaatggga cgagttcagg
     9361 aatcgagcgt ctcagaacga aagggacgca gaggttcagg cctcccgcgt ggagcacctc
     9421 gatcagctcc ttcagcgtct tcgaggccgc cacgaccagc tgcttgacga tgcccaggag
     9481 gtgcccgttc ttgccagcga cgacgtggcg tccctggccg aagagatagc cggtatcgaa
     9541 tcgcggcgga gcggcctgga ggccgacacc gatcgcacca tcaaggccct tggctccatc
     9601 cgggaagatc tgctggttcg ggagcgcatg gtcgaggagg cccgtggtga gctgcagtcg
     9661 ctcagacacg atctcgccag cctgcaggcc gttcagcgtg ccgccctggg ccgtgacgag
     9721 cgttctgcgc aggattgggt cgacgcccga ggcctcggct cggccgagcg tctgggtgaa
     9781 gctctggcgg tggtgcccgg atgggaacat gccgttgaat cggtgctggg cgacgatctt
     9841 cagggcatac gcgttgacga tgtgagcccg ttcggcgttg ctctggatca cctgccgggc
     9901 gccggcgtga ccctggtgga aggaagtttc cacgccgaga gcagcggaga gctgccgagc
     9961 ctttcctccc tcattagatc cgggagtctc aagctcgatt ccctgcttta tgggattttc
    10021 gcggcggagt ccgtagaggt ggcttttgcg caccgcggcg ggttgcgggc cggccagagc
    10081 atcattaccc gccaggggat ctgggtcgga cccgactggg tccgtatcct gccggtcagc
    10141 gagcaggatt cgggcatcat ccagcggact caggagctgg aaaccctcgg gctgcgggtc
    10201 gaggaagccg agctcggcct tgccgagctg caacgccagg tcgtggaagg gcgcgggcag
    10261 gccgaggagc tcgaacacga tcgagcgaag ctgcaggcag aggtcaacga cctgaacgtt
    10321 cggctcggcg acatgaaagc ccagcacggc gtgaaaaggg tgcagatcga ggaggccggc
    10381 gcccgtcggg agcggatccg tcgcgagcgc gaggagctcg acgctcaggt ttccgaggag
    10441 acgcgcaagc ttctggaggc aagggaagcc ctggccatcg ccgagcggac cagagagcaa
    10501 caggacgtca cccgtgagga gctccaagct cgtggtgccg cgtcggagtc ggagcttgcc
    10561 gcggcccggg aagaagcccg ggccaatcgt gagcggtacc acgctctcaa cgccgagtgg
    10621 cagagcctgg agtcccgcat gtcggccagc gagaccgcgc ggggccggct ggttcgccag
    10681 cgccgcgagc tggacgagca gcgtgacaac cttcaggccg gcatcgcctc cagcgccacg
    10741 ccgctgcccg agctgcgcca ggacctggag gccaggctga acgagcggtt ggaagttgag
    10801 cggcagctcg gcgcggcgcg cgccgagctc gaacagcttg gcgagcggat tcgcgggctt
    10861 caggcagagc gtggcgaagc ggaagcagcg gtggagaccg ttcgctcgaa gctcgagacc
    10921 acgcgggtcg agcgccaggg actcgccgtg cagcagtcca acctcacgga tcagatcgcc
    10981 gccaccggcc tcggcgcgga cgaggtgcgc gaggggctgg ccgaggatgc ggaagaggcc
    11041 cgctgggtcg agctgctcga aaggaccgat cggcgcatcc agcgccttgg ccccatcaac
    11101 ctggctgcca tcgaggagtt cgaggcgcag tcggagcgga aggtctacct cgacaaacag
    11161 aacgaggacc tggagcaggc gctggaaacc ctgctcggcg caattcgcag gatcgatcgc
    11221 gagactcgcg cacgcttcaa ggaaaccttt gagacggtga acagacatct gggagagctg
    11281 ttcccgaaag tattcggcgg aggacacgcc tatctggagc tgacagggga ggatctgctc
    11341 gacaccgggg tttcgctcat ggcgaggccg ccgggcaaac gcaattccag cgttcacctg
    11401 ctttcgggcg gggagaaggc gatgacggcg gtggctctga tttttgccat cttccacctc
    11461 aacccgagcc cggtctgcat gctcgacgag gtggacgcgc cgctcgacga cgtgaacgtc
    11521 atgcgttttg ccgagctgat taaggatatg tctaccgacg tgcagtttgt tgtgatcacc
    11581 cacaacaagc tcaccatgga gatggccgac catctcatgg gcgttaccat gaacgagccc
    11641 ggcgtatcgc ggctcgtctc ggtagacgta gaagaagcct cggcgatggc tggctgagcc
    11701 atgtcgccgg aagcttcacc ctctggacgt gtcaggtcgc ccaggcggcc gaggagccgg
    11761 ttgtggacat caaagatttc attctgatcg gcggcggcct gctgatcgcc ggggtgattg
    11821 cccacgggtt ctacattgcc tggcgcggtc gtcgcgatac cctgcgcatg gacattgctc
    11881 acgacctgat tcccgaggcc gtcgacgatc ttcatcggtt gcgcggcgag ctgcccaacg
    11941 ggggcgcccg cgtggtgtcg caggcgtctc tcggtgagca ggcaagcctc gagctcgaca
    12001 ccgtggtggt ggctgagccc ttggacgcgc agccgcccga gcctgcgatg ctggccgaag
    12061 agagcgcggt cg
//
LOCUS       DBNHOX010000071         2030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_777245, whole genome shotgun sequence.
ACCESSION   DBNHOX010000071 DBNHOX010000000
VERSION     DBNHOX010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_777245"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..494)
                     /locus_tag="AC16UZ_01615"
     CDS             complement(117..494)
                     /locus_tag="AC16UZ_01615"
                     /inference="COORDINATES: protein motif:HMM:NF015953.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR6245405.1"
                     /translation="MIGIILRILITALGLWAAASIIPGVQIDSTGTMVWAAIWLGVVN
                     AVIRPVAIILTLPLTLISLGGFLLVVNAAMFGLVAAMLNDFHVSGFFAALFGALIVSI
                     TSWLGSSFIGADGRYEVMVIRRD"
     gene            complement(538..963)
                     /locus_tag="AC16UZ_01620"
     CDS             complement(538..963)
                     /locus_tag="AC16UZ_01620"
                     /inference="COORDINATES: protein motif:HMM:NF042761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HIR6245406.1"
                     /translation="MSALLAGVIFGLGLSISGMADPTIVLAFLTLGPGWNASLLVVMA
                     AAVTVTTIGYRLAGRRPAPLLDSEFHAPARRDIDQRLVIGAVVFGVGWGLAGYCPGPA
                     IVGALTLDPRALAFLPGFVVGMIAFEAVPKPSARQPEQA"
     gene            complement(972..1373)
                     /locus_tag="AC16UZ_01625"
     CDS             complement(972..1373)
                     /locus_tag="AC16UZ_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015095134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HIR6245407.1"
                     /translation="MTNAIIGGLLIGAAAVWLWISLGRIAGISGIAAQALTRPRQSLW
                     PLLFVAGLGIGGWLGQGVRHPEAIPELGVDSIVLLTLGGLLVGFGTRLGSGCTSGHGV
                     CGIARFSRRSLAATVIFVGMGMLTATLVHGT"
     gene            1633..>2030
                     /locus_tag="AC16UZ_01630"
     CDS             1633..>2030
                     /locus_tag="AC16UZ_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6245408.1"
                     /translation="MEDVYIVEAVRSPIGKRGKGLAGLMPADLLGAVQKAALERSGIA
                     PEKVGQVVGGCVSQVGEQSFNIARIAWLSQGLPEEVAATTVDSQCGSSQQATSLGAAM
                     VASGMENVVMCCGVEMMTRVPLGSNMKDGVP"
BASE COUNT          381 a          631 c          710 g          308 t
ORIGIN      
        1 atcagagtaa tgaaatttcg ctgcatcggc tgaatctctc cgggaacttg agcatcatct
       61 gaccgcggcg gattatcggc cagggggatg agccttgtcg aaccgggtgg cgcgggtcaa
      121 tcccgtcgga tgaccatcac ctcgtagcga ccgtcggccc cgatgaagct cgagcccagc
      181 cagctggtga tgctcacgat gagcgctccg aacagcgcgg cgaagaaacc gctgacgtgg
      241 aagtcgttga gcatggccgc caccagaccg aacatggccg cgttgacgac gagcagaaag
      301 ccgcccaggg agatgagcgt caggggcagg gtgaggatga tggccactgg ccggatgacg
      361 gcgttcacca ccccgagcca gatggccgcc cagaccatgg ttccagtgct gtcgatctgc
      421 accccgggaa tgatgctggc ggccgcccaa agaccgagag cggtgattag gatcctgaga
      481 atgatgccaa tcatggtctc attctgcgcc ctacgggcgg cgacggccag cgggccttca
      541 ggcctgttcc ggctgccggg cggatggctt cggcacggcc tcgaaggcga tcatccccac
      601 cacgaagccg ggcaggaagg cgagcgccct cgggtccagc gtcagggctc caacgatggc
      661 gggacccgga cagtaaccgg ccagccccca gccgacgccg aacaccacgg cgccgatgac
      721 gagccgctgg tcgatgtccc gccgcgcggg cgcgtgaaat tcgctgtcga gcagtggtgc
      781 cggtcgtctt cccgccagcc ggtagccgat ggtggtgacc gtcacggcgg ccgccatgac
      841 gacgagcagc gaggcgttcc aacccggacc cagagtcagg aacgcgagca cgatggtggg
      901 gtccgccatg ccggagatgc tcagacccag cccgaatatc actccggcca acagggctga
      961 cacgggcccc atcatgttcc gtgcaccagg gtagccgtca gcatgcccat gccgacgaag
     1021 atgacggtgg ctgccaggga acgtctggag aagcgggcga tcccgcaaac gccgtgaccg
     1081 ctggtgcagc ccgaacccag gcgggtgccg aagccgacca gaagcccgcc cagtgtaagc
     1141 aggacgatgc tgtcgacgcc gagttccggg atcgcctcgg ggtgacggac gccttgcccc
     1201 agccagccgc cgatgcccag gccggcgacg aacaggagcg gccagaggct ctgtcgcggt
     1261 ctcgtcagcg cctgggcggc aattccgctg atccctgcga tgcggccgag gctaatccag
     1321 agccacacgg ccgcggcgcc gatgagcagc ccgccgatga ttgcgtttgt cacgactttc
     1381 cggtcggtta cgtgagggtg ggcgatcttg ccacgaagag cgcaacctgc ctagttcccg
     1441 gcgtaacccg ggatgagtcc cggtagtaaa gtgtggccag gatctagaga ggatctgcgg
     1501 agtgcagacg actgacggtg cggaaattct gattgaggat cggggttcgc gaagctgagc
     1561 gtccggaaac ccgtggcgag ctgccctggc tcgggtagaa tgcggcgctc tcgtccaggg
     1621 agttcgtaag caatggaaga cgtctacatc gtcgaggccg tccgcagccc catcggcaag
     1681 cgtggtaagg ggcttgccgg cctgatgccg gccgatctgc tcggagccgt tcagaaggcg
     1741 gcgctggagc gcagcggcat cgcgccggag aaagtcgggc aggtggtggg cggctgcgtg
     1801 tcccaggtgg gcgagcagtc gttcaacatc gcccggattg cctggctgtc ccaggggctg
     1861 cccgaggaag tggcggccac caccgtcgat tcccagtgcg gctccagcca gcaggccacg
     1921 tccctggggg cggccatggt ggcctccggc atggagaacg tggtcatgtg ctgcggcgtg
     1981 gaaatgatga ctcgggtccc gctgggatcg aacatgaagg acggcgtgcc
//
LOCUS       DBNHOX010000072         2222 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_262910, whole genome shotgun sequence.
ACCESSION   DBNHOX010000072 DBNHOX010000000
VERSION     DBNHOX010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_262910"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..189)
                     /locus_tag="AC16UZ_01635"
     CDS             complement(61..189)
                     /locus_tag="AC16UZ_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245409.1"
                     /translation="MRNINPRTWNDVTEPMLRAEGLGGLLALLFLLLVVVELFWAG"
     gene            357..>2222
                     /locus_tag="AC16UZ_01640"
     CDS             357..>2222
                     /locus_tag="AC16UZ_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014896.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HIR6245410.1"
                     /translation="MMSRIRLAAIATGLLFVTAVVSAEAPPIAEKPVVETHFGTRVID
                     PYRYLENVADPEVTGWMKAQSAHARATLDAIPGREDLLKRLVDLQGKRAATVAGIIEC
                     PGERLFYMKRGIGQNVFKVYLRDGFAGSERLLVDPEVLQAKTGVPHAVNYFSPSWDGR
                     YLAYGVSAGGSEDASLHIMDVATGDEVGEPIPRVPHGGVSWTPDSKTIVFNRRKELTA
                     AMPDTERYLDSVVYALQLGKPLSSAKPVFSAALLPELKLRRLDNASLLFQPGSPFVVA
                     VSNDTTERDDNLFVLPLAEFGRDNAPWVRIASTSDEIADVALRGNDLYLYTHSAAPRF
                     RIARLDLREPVLGKAVDVVPEQAGVIAGFSLGRDAIYFQLRNPFGTELVRAPYEGLAP
                     APVKLPVEGTVELYGDRAQLRSGAIVYATSWVDAPSVLVWDARTGKSDDTGLAEPGSI
                     RSLTDVVVERVMVPSHDGVKIPLTILYRKGLARDGQRPTVVYGYGSYGISTDPTFSTS
                     LYVWLEQDAVLAIAGVRGGGELGSAWRDAGTGANKPNTWKDGIACAEYLVAKGYTSPG
                     KLAVRGGSAGGIFAGRAITERPDLFRVGVIQVGMTDTLRAEFSANGVTNISEFGTV"
BASE COUNT          400 a          695 c          719 g          408 t
ORIGIN      
        1 caggccatcg attctaattc gcgaacgacc cgcgtcgctc atctggcttg tcccagcgcg
       61 tcagccggcc cagaaaagct cgacgacgac cagcagcagg aacagaagcg ccaacagccc
      121 gccaagccct tctgcccgca gcatcggctc cgtgacgtcg ttccaggtcc tgggattgat
      181 attccgcatg cacttcatcc tcttggcctg cgcagcccct ctcattggtt agaccattga
      241 cggcggcatc cgtttaggaa agtgctgttt cgggacgggc cggttggctg tctagaatat
      301 cgaccatgaa tgacgcaccg cagcatgccc gcggggcgtc ctgacaggag aatgaaatga
      361 tgtcgcgcat ccgcctggcc gcaattgcta ccggtctctt gttcgtcact gcggtggtct
      421 cggcagaggc tcccccaatt gccgagaagc ccgtcgtcga gacgcatttc ggcaccaggg
      481 tgatcgaccc gtaccgctac ctcgagaacg tcgcggatcc agaggtgacg ggctggatga
      541 aggcgcagag cgcccacgcg agagcgacgc tcgacgccat tccaggccgc gaggatctgc
      601 tcaagcggct cgtcgatctc cagggcaagc gtgccgccac ggtggccggg atcatcgaat
      661 gcccgggcga acgccttttc tacatgaagc ggggcatcgg ccagaatgtc ttcaaggtgt
      721 acctgcgtga cgggttcgca ggcagcgagc gactgctggt tgatccggaa gtcctgcagg
      781 caaaaaccgg cgtcccccac gcggtcaact acttttcgcc atcctgggat ggccgctacc
      841 tggcctacgg cgtgtccgcg ggtggctcgg aggatgcgtc gctgcacatc atggacgtgg
      901 ccaccggcga cgaggtcggt gagccgatcc cgcgtgtacc gcatggtgga gtttcgtgga
      961 cgccggacag caagacaatc gtcttcaacc gacggaagga actcaccgct gcgatgccgg
     1021 acacggagcg ctacctggat tccgtggtat acgccctgca gctgggcaag cctttgtcga
     1081 gcgccaagcc ggtcttcagc gcggccttgc tgccggaatt gaaacttcgc cggttggaca
     1141 acgcctccct gctctttcaa cctggcagcc catttgtcgt cgcggtcagc aacgacacga
     1201 ctgagcgtga cgacaatttg tttgtcctgc cgctggcgga atttggccgc gacaacgccc
     1261 cctgggtgcg gatcgcaagt acgtccgatg aaatcgccga cgtagcgctc cggggtaacg
     1321 acttgtacct gtacacgcat tcggcagcgc cgcgattccg tatagcgcgg ctagatctgc
     1381 gggagcccgt cctcggcaag gccgtcgatg tcgtgccgga acaagccggg gtcattgccg
     1441 ggttttcgct cgggagggac gccatctact tccagcttcg caaccccttc ggtaccgagc
     1501 ttgtccgtgc cccctatgaa ggcctggcgc cggcgccagt aaagttgcct gtggagggta
     1561 ccgtggaact ctatggggac cgggcccagt tgcgttccgg cgcgatcgtg tacgcaacat
     1621 catgggtcga cgctccgagc gtgctcgtct gggatgccag gacgggcaag tccgacgaca
     1681 ctggtctcgc cgagccgggc agcatccgct ccctgacgga cgtcgtcgtc gagcgggtca
     1741 tggtgcccag ccacgatggg gtgaagattc cgctgacgat cctctaccgc aaggggctgg
     1801 cccgtgacgg tcagaggccg acggtcgtct atggctacgg cagttacgga atcagtaccg
     1861 atccgacctt cagcacgagc ctgtacgtct ggctggagca ggatgccgtg ctggcgatag
     1921 ccggcgtccg cggcggcgga gaactgggca gcgcctggcg ggatgccgga acaggcgcca
     1981 acaagccgaa tacctggaag gacggcattg cctgtgccga gtacctggtg gcgaagggct
     2041 acacgtctcc cggcaagctg gcggttcggg gcggcagtgc cggcggcatc ttcgccggaa
     2101 gggcgatcac cgagcggccg gacctgtttc gcgttggcgt gatccaggtg ggcatgaccg
     2161 acaccctgcg cgcggaattc agcgccaacg gcgtcacgaa catttcggag tttggcaccg
     2221 tg
//
LOCUS       DBNHOX010000073         1720 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_327533, whole genome shotgun sequence.
ACCESSION   DBNHOX010000073 DBNHOX010000000
VERSION     DBNHOX010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_327533"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="AC16UZ_01645"
     CDS             <1..389
                     /locus_tag="AC16UZ_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6245411.1"
                     /translation="EPKVGLAALAGGLNRLPRQIGPKRALGMILTGRHVPAEEGLQLG
                     FVNEVVPHDELLAATRRWAEQICACAPLSIRASKDVVYRSLDSASLQASMQAQYDSVK
                     AMVKSEDFIEGPKAFAEKRPPAWKGR"
     gene            complement(415..612)
                     /locus_tag="AC16UZ_01650"
     CDS             complement(415..612)
                     /locus_tag="AC16UZ_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245412.1"
                     /translation="MKDPAVRSYPEQFATTPDLLHVVIPRIEIIAEMAVRDERDALND
                     VCLAVACVADAIGSAQLAAQG"
     gene            complement(862..1593)
                     /locus_tag="AC16UZ_01655"
     CDS             complement(862..1593)
                     /locus_tag="AC16UZ_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4336 domain-containing protein"
                     /protein_id="HIR6245413.1"
                     /translation="MRLFDRALSRLHPEQLPVMGARLRSIGRQIWVADRELQMPGGIR
                     LPIRMVVMGDGRGNLLCYSPVELDEGTAEALDGLGKVRWIVAPNRYHRSFTAGCMSRY
                     PQAQVSGFLPPPGDGFAAPAAVPFGGCELTTVSLRTGFAEIVAYHDLSETLVVADLLF
                     NVRNGNWRLQAVMRVNGAWQKAGHTRLQRWLILRDPDGLTKFRRWALSRPFVQISMAH
                     GLIVQEDARECLYRLQRGGPFGAPH"
BASE COUNT          328 a          567 c          535 g          290 t
ORIGIN      
        1 ccgaacccaa ggtcggtctt gctgcgcttg ccggcgggtt gaaccggttg ccgcggcaga
       61 tcggcccgaa gcgcgcgctc ggcatgattc tcaccggccg tcacgtgcct gccgaggaag
      121 gattgcagct cggcttcgtc aacgaggtgg ttccacacga tgagctgctg gccgccacgc
      181 gccggtgggc ggagcagatc tgcgcctgcg cccccttgtc gatccgagcc agcaaggacg
      241 tggtctatcg cagcctcgac tccgcttcgc tgcaggcgtc catgcaggcc cagtacgaca
      301 gcgtcaaggc catggtcaaa agcgaggatt tcatcgaggg tccgaaagcg ttcgcggaga
      361 agcggccgcc ggcgtggaag gggcgctaaa gccaggaaaa ttccagcggc ggcgctatcc
      421 ctgcgccgcc aactgggcgg agccgatggc atccgcgacg caagctacag ccaggcagac
      481 atcgttgagc gcatcgcgct cgtcccgcac tgccatctcg gcgatgatct ctattcttgg
      541 aatgaccacg tgcagcaggt ccggggtggt cgcaaactgc tcggggtatg acctgactgc
      601 cggatctttc atgctctgcc tctcgtccat ctcccgctaa gcggccgtca gcacatggca
      661 ttcaggtttc gtgccagtct aaaatacctt gataagccat tgttttatat atttttattt
      721 ccatatggcg ccacgattcg aaacgtgccg aaggtgacgc ttgggagaag aaactgaccg
      781 ggccgggccc ggacggagcc aaccggccgc tattgcctgc agagtcctgg ccggccgccg
      841 accccacacg cgatcagcgt ttcagtgggg cgctccgaac gggcctcccc gctgcagccg
      901 gtaaagacac tcccgcgcat cctcctgcac gatgagaccg tgagccatgg agatctgcac
      961 gaagggcctg gacagcgccc accgacggaa cttcgtcagg ccgtcgggat cgcgcagaat
     1021 cagccaccgc tgaagcctgg tgtgtcccgc tttctgccac gctccgttga cgcgcatgac
     1081 cgcctgcaac cgccaattgc cgttgcgcac gttgaacagc agatcagcaa ccacgagggt
     1141 ttcggagagg tcgtgatagg cgacgatctc cgcgaagccc gtacgcaggg acaccgtggt
     1201 cagctcgcat cctccgaagg ggacggcggc cggggcggcg aacccgtcgc cggggggcgg
     1261 caggaaaccc gacacctgag cttgcgggta gcgggacatg cacccggcag tgaaggaacg
     1321 atggtagcgg tttggcgcaa cgatccagcg caccttgcca aggccatcca gtgcctcggc
     1381 cgtcccttca tccagctcca ccggggaata gcagagcaga ttgcctctgc cgtcacccat
     1441 caccaccatc cgaatgggga gcctgattcc gcccggcatc tgcagctcgc gatcggccac
     1501 ccagatctgc ctcccgatgg accgaagccg cgcgcccatc accgggagct gctccgggtg
     1561 caggcggctc agcgcccggt cgaagaggcg cacgaacatc tgcccgatca aagtgctttc
     1621 acttggaatc gatcagctcg gcaagctgct ccagctggtg gaggtacgcg tccgcatcgt
     1681 catagccaag ggcacagacg agccggtcca gcccgaggcc
//
LOCUS       DBNHOX010000074         1838 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1548640, whole genome shotgun sequence.
ACCESSION   DBNHOX010000074 DBNHOX010000000
VERSION     DBNHOX010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1548640"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..146
                     /locus_tag="AC16UZ_01660"
     CDS             21..146
                     /locus_tag="AC16UZ_01660"
                     /inference="COORDINATES: protein motif:HMM:NF017315.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HIR6245414.1"
                     /translation="MTPIVTGSFDTITEGLPNARIGDITACGAFIVTGSPDTIDN"
     gene            157..465
                     /locus_tag="AC16UZ_01665"
     CDS             157..465
                     /locus_tag="AC16UZ_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245415.1"
                     /translation="MNNLEQPQEPHYWDVFPKLIRVSRSTFVQRIPLSIRGLPEAPVF
                     ESSNPDVASVDEDGNVECGFVPGAAMILVWDSPERLSLRHVQVEVYGGGVSAPVEVPS
                     "
     gene            462..1472
                     /locus_tag="AC16UZ_01670"
     CDS             462..1472
                     /locus_tag="AC16UZ_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245416.1"
                     /translation="MTDAPAAFSHWEVQPSSIRLSAGEFEQRIPLSLRGDVDAPVFAT
                     SNPEVAEIGPDGVIRCGWTIGNAVLMVWRSSARDSLRHVLVEVRDPSWFANHPDFASG
                     ATVFLSGTVVNALNTSGVGNALIEFRRSETGPAAFQTFANAYGGFELSVPEGFYYVEV
                     TAPGYIAWHGWVNADPNTSGDIQIVLSPELDGQVARIVLQWGLNPRDLDSHLTGPTPS
                     GGRFHVFYSHTIENEAAELDVDDTSSYGPETITIHRLIPGVYRYAVHDYTNRNANPST
                     GLAQSGASVKVFLSDGREQTFTVPNAPGTVWTVFEIDGATGTVTPVNGMSYQSQPANV
                     GM"
     gene            1485..>1838
                     /locus_tag="AC16UZ_01675"
     CDS             1485..>1838
                     /locus_tag="AC16UZ_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245417.1"
                     /translation="MISEEPADLQATIEQTDSGEQQYVLRALCDHLSGIREELSGIRT
                     LLEASHSASEAMRGQAQAYLEAQQARTQEYLDQVQIEPEPDFYPFVELPAGTEPRDLP
                     DGNRLFTLPDGMILRT"
BASE COUNT          336 a          624 c          538 g          340 t
ORIGIN      
        1 gtcccatccc ggggcatggc gtgacgccca tcgtgaccgg cagcttcgac accatcaccg
       61 aaggattgcc caacgcccgc atcggcgaca tcaccgcctg cggagccttc atcgtcaccg
      121 gcagtcccga caccatcgac aactgagggg gacgccatga acaatctcga acagccgcag
      181 gaaccgcact actgggatgt cttcccgaag ctgatccggg tctcgcgatc cacattcgtt
      241 cagcgcattc cgctctcgat ccgtggcctg cccgaagcgc cggtgttcga atcgtccaat
      301 cccgacgtgg ccagtgtcga tgaggacggc aatgtcgaat gcggcttcgt tcccggagcg
      361 gccatgattc tggtctggga ttcgcccgag cggctcagcc tgcggcacgt tcaggtcgag
      421 gtctatggcg gcggcgtctc cgcaccggtg gaggtccctt catgacggat gccccggcag
      481 ccttcagcca ctgggaggta cagcctagct ccatccgcct ctccgctggc gagttcgagc
      541 aacggattcc gctctccctg cgcggcgacg tggacgctcc ggtctttgcc accagcaatc
      601 cggaggtcgc ggagatcggg ccggacggtg tcattcgctg cggctggacc atcggcaacg
      661 ccgtgctcat ggtctggcga tcctcggccc gggacagtct ccgccatgtt ctggtggagg
      721 tccgcgatcc gtcctggttc gccaatcatc cggactttgc cagcggagcc acggtcttcc
      781 tcagcggcac ggtagtcaac gccctcaaca ccagcggcgt cggcaacgcg ttgatcgaat
      841 tccgccgctc ggaaaccggc ccggcggcgt ttcagacctt cgccaacgcc tatggcgggt
      901 ttgagctgtc cgtgcccgag ggattctatt acgtggaggt caccgcgccg ggatacatcg
      961 cctggcatgg ctgggtgaac gccgacccca acacctccgg cgacatccag atcgttcttt
     1021 cgcccgagct cgacggccag gtcgcccgca tcgtgttgca gtggggcctg aatccccgag
     1081 atctcgattc ccatctcacc ggaccgacgc catcgggcgg tcgttttcat gtgttctatt
     1141 cccacaccat cgaaaacgag gcggcggaat tggacgtgga cgacaccagt tcctatgggc
     1201 cggagaccat caccatccat cggctcatcc cgggtgtcta ccgctacgcg gtccacgact
     1261 acaccaaccg caacgccaat ccgagcaccg gactggcgca gtccggagca tcggtgaaag
     1321 tgttcctgag cgatggccgt gagcagacct tcaccgttcc caacgccccg ggcacggtct
     1381 ggaccgtgtt cgaaatcgac ggcgcgaccg gaacagtgac gccggtcaac ggcatgagct
     1441 atcaatccca acccgccaat gtcggcatgt aatggaggtt gtccatgatt tccgaagaac
     1501 ccgccgactt gcaggccact atcgaacaaa ccgattccgg cgaacagcag tatgttttac
     1561 gggcgctctg cgatcacctg tcaggaatcc gtgaagaact ctctggcatc cgcacgctgc
     1621 tggaggccag tcactccgcc tctgaggcga tgcgtggcca ggctcaggcc tatcttgagg
     1681 cccagcaggc caggacccag gagtatctgg accaggtaca gatcgagccg gagcccgatt
     1741 tctatccctt cgtcgaactg cctgcgggca ccgaaccccg ggatctgccg gacggcaacc
     1801 ggctcttcac cttgcccgac ggcatgatcc tgcggacc
//
LOCUS       DBNHOX010000075         4789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_520331, whole genome shotgun sequence.
ACCESSION   DBNHOX010000075 DBNHOX010000000
VERSION     DBNHOX010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_520331"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="AC16UZ_01680"
     CDS             complement(<1..1221)
                     /locus_tag="AC16UZ_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012946.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6245418.1"
                     /translation="MSEARTEPAVRVDPATGVKRFATRAAKATDKIAGKGYSVIADEA
                     LTTLPPAPPGAVFDAEEQAKYRAFKEVRRGAADYMTMEGEFARYLEDVYSADPVPREA
                     LTDECEVLVVGAGFAGLLLWQKLSAAGFTDVRFCEKGGDVGGTWYWNRYPGIACDVES
                     YSYLPLLEEMGYIPSMKFASGFEILEYCQRMAEKFGFYDRCLFHTTVDRTEWDEAAGR
                     WTVYTNRGDAMRARFVVLANGILTTPQLARIDGMETFKGESFHTSRWNYHVDLAGKRV
                     GIIGTGATAVQVIPEIAKIVKELYVFQRTPSSIDVRDQRETTPEEYETWSHEPGWARA
                     RRARFSKISSGRTAIKANDDYLSGKVADFKERKQHERRLSAEEMINKQLETNFRIMEQ
                     IRARVDAIVEDPRTA"
     gene            complement(1218..2444)
                     /locus_tag="AC16UZ_01685"
     CDS             complement(1218..2444)
                     /locus_tag="AC16UZ_01685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6245419.1"
                     /translation="MIDFEIPAETKAIRAKAREFVQKECVAAEAQCTADNFDQVLSEL
                     RGKARAQGLWCPFIPEEHGGMGLKPLANALVQMELGESFLGALALNTQGPDDATMLTL
                     LEHGTDHQKEKFLKPLLDGQKRICYSMTEKAAGADATGMQTAAVLEGDNWVLNGEKWF
                     SSSADVADIAVVMAKTDPEAPRHERYSTFIVELPNPGYQILRNIDTMQPHTELGLKLG
                     HSHSEVKIENLVVPRENLLGGRGKGFAMGQHRLAYGRLRHGMHNVAMAQRALDLAAGH
                     VVNRETFGKRLADRQGVRWMLADCAAEIYKARLMLLHIAYKAERGMDLRQENGIAKVF
                     LAHMVHQVVDTALQLHGALGYSHDTPLASWYTSIRSQRLVDGPDEVHRWRTGANVIKA
                     YEEHGTTAPACGGELL"
     gene            complement(2538..3869)
                     /locus_tag="AC16UZ_01690"
     CDS             complement(2538..3869)
                     /locus_tag="AC16UZ_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HIR6245420.1"
                     /translation="MTSILGRFPAVLVLLSLADAGLVPEAGAMPDEPVKVEVVRTARG
                     YALERGGRPYRIQGVGGLERLALLAESGGNSIRTWSVGTPEQSARELLDEAHRLGLTV
                     ALCLDIARERHGFDYDDDAAVAAQFERARADVLAHRNHPALLAWIIGNELNHDFKNPR
                     VYDAVNEISRMIHEIDGNHPTTTTIAGFSADMAEVISARAPDIDFLSIQSYAGVLDLQ
                     RRVRKAGYEGPYVLTEWGPPGHWEVATTSWGAPIEPDSSAKAKALRRAHATAIEPYPD
                     QLIGNYVFLWGQKQERTPTWYGLFTETGERTESVDVMQHLWTGAWPSNRSPAVRSVRL
                     DGRRAGRDIVLVPGQTYPILARVRDPEGDALRYRWSVKSESRARQVGGDLEGAIADVD
                     GLIDEPSSSRAQLTAPEEPGAYRVFVYAYDGNGGAAHANVPFLVRSPAGAD"
     gene            complement(3866..>4789)
                     /locus_tag="AC16UZ_01695"
     CDS             complement(3866..>4789)
                     /locus_tag="AC16UZ_01695"
                     /inference="COORDINATES: protein motif:HMM:NF037461.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HIR6245421.1"
                     /translation="LSFSALFPEGLALLKKLKGDGIPVVSVFLTGRPLWVNPELNASD
                     AFVVAWLPGSEGAGVSDVLFRTVGGEVAHDFTGRLAFSWPRSSLQATVNRHDADYDPL
                     FPYGFGLGAADVDTLGDELIEEDEALGLAADESIGILTGRVVPPFEFFIGDSQAWKVP
                     VRGRSSRSPTGAVSVTNVDHYSQEDARRISWDGSGGAQVYFQRSAGQDLSDHASRGAR
                     LRCKLRVDVRPQRRVELRMDSGYPQTASIDITAMLNELPLGTWEDVAFEMSAFAGGTD
                     NLCAVDTPWLIWTDGVLGLSVSNLRIDLGDG"
BASE COUNT          757 a         1633 c         1555 g          844 t
ORIGIN      
        1 ggccgtcctg gggtcctcga cgatggcgtc cacgcgggcg cggatctgct ccatgatgcg
       61 gaagttggtc tcgagctgct tgttgatcat ctcctcggcg gagagcctgc gctcgtgctg
      121 cttgcgctcc ttgaagtcgg ccacctttcc cgacaggtag tcgtcgttgg ccttgatggc
      181 cgtccgcccg gaggagatct tcgagaatcg cgcgcgccgc gcccgggccc agccgggttc
      241 gtgcgaccag gtctcgtact cttccggcgt ggtctcccgc tgatcgcgca cgtcgatgga
      301 cgacggcgtc cgctgaaaga cgtagagctc cttcacgatc ttggcaatct cggggatcac
      361 ctgaacggcc gtggcgccgg tgccgataat gcccacccgt ttgccggcca gatccacgtg
      421 atagttccag cgtgatgtgt ggaaggactc gcccttaaac gtctccatac cgtcgatacg
      481 cgcgagctgg ggcgtggtga ggatgccgtt ggccagcacc acgaagcgcg cgcgcatggc
      541 gtcgccgcga ttcgtgtaaa ccgtccagcg accggccgcc tcatcccact ccgtgcgatc
      601 cacggtggtg tgaaacaggc agcgatcgta gaagccgaac ttttccgcca tccgctggca
      661 gtactcgagg atctcgaaac ccgaggcgaa cttcatgctg gggatgtaac ccatctcctc
      721 cagcagcggg aggtagctgt aggactcgac gtcgcaggcg atgcccgggt agcggttcca
      781 gtaccaggtg ccgcccacgt cgccgccctt ctcgcagaag cgcacgtcgg tgaatccggc
      841 tgcggaaagc ttctgccaaa ggagcagacc ggcgaaaccc gccccgacca cgagcacctc
      901 gcactcgtcg gtcaacgctt cccggggcac cggatccgcg gaatagacgt cctctaagta
      961 cctcgcgaac tcgccttcca tggtcatgta gtcggcggcc ccgcgacgga cctccttgaa
     1021 ggcccggtac ttcgcctgct cctcggcgtc gaacaccgcg cccggcggag cgggcggcag
     1081 cgtcgtcaac gcctcgtcgg caatcaccga atagcccttg cccgcgatct tgtccgtggc
     1141 ctttgccgcg cgggtggcaa agcgtttgac cccggtcgcg ggatccacgc gaacggcggg
     1201 ctctgtccgg gcctcgctca cagcagctcg ccgccgcagg cgggcgccgt ggtaccgtgc
     1261 tcctcgtaag ccttgatcac gttggcgccc gtgcgccagc ggtgcacctc gtccgggccg
     1321 tccacgagcc gctggctgcg gatgctcgtg taccagctcg ccagcggcgt gtcgtggctg
     1381 tagcccagcg ccccgtgcag ctgcagcgcg gtgtccacca cctgatgcac catgtgcgcg
     1441 aggaacacct tggcgatccc gttctcctgc ctcaagtcca tgcctcgctc ggccttgtag
     1501 gcgatgtgca gaagcatcag ccgggccttg tagatctccg ccgcgcagtc ggccagcatc
     1561 cagcgaacgc cctgcctatc ggcgagccgt ttgccgaagg tctcccgatt gaccacgtgc
     1621 ccggcggcca gatccagcgc ccgctgcgcc atggccacgt tgtgcatgcc gtgacgcagc
     1681 cggccgtagg caagccggtg ctggcccatg gcaaagccct tgccccggcc gccgaggagg
     1741 ttctcgcgcg gtaccacgag gttttcgatc ttcacctcgg agtgagagtg gccgagcttg
     1801 aggcccagct cagtgtgggg ctgcatggtg tcgatgttgc gcaggatctg atagcccggg
     1861 ttgggcagct cgacgatgaa cgtgctgtag cgctcgtggc gtggcgcctc cgggtccgtc
     1921 ttggccatca ccacggcgat gtcggcgacg tcggccgacg agctgaacca cttctcgccg
     1981 ttgagcaccc agttgtcgcc ctccagaacg gccgcggtct gcatgcccgt ggcgtccgca
     2041 cccgccgcct tctccgtcat cgagtagcag atgcgcttct ggccgtccag caggggcttc
     2101 aagaactttt ctttctggtg gtcggtgccg tgctcgagca gcgtgagcat ggtggcgtca
     2161 tccggaccct gggtgttgag cgcgagcgcg ccaagaaagc tctcgccgag ctccatctga
     2221 accagggcgt tggccagcgg cttcagcccc atgcccccgt gctcctcggg gatgaacgga
     2281 caccacaggc cctgggcgcg ggccttgcct ctgagctccg aaagcacctg gtcgaaatta
     2341 tccgccgtgc attgcgcctc cgccgccacg cattccttct gcacgaactc gcgcgccttg
     2401 gcgcggatgg ctttggtctc ggccggtatc tcgaaatcga tcattggtcc ctttccctat
     2461 gcaggttcgt gtctgtcgtg actatatcag gagcacgggc gcgggaacca cggcaggccc
     2521 cgaaaggggt ggtgcgttca gtcggctcca gccggagaac ggacgaggaa ggggacgttg
     2581 gcgtgcgccg cgccgccatt gccgtcgtat gcgtagacga agacgcgata cgccccgggc
     2641 tcttcggggg ccgtcagctg cgcgcggctc gacgacggtt cgtcgatcag cccgtccacg
     2701 tcggcgatcg ccccctcgag gtctcctccg acctgcctgg cccggctctc tgacttgacg
     2761 ctccagcgat agcgcagggc gtccccttcc ggatcgcgca cccgggcgag aatgggatac
     2821 gtctgccccg gaacgaggac gatgtcccga cccgccctgc gaccgtcgag gcgaaccgat
     2881 ctgaccgcgg ggctgcgatt gctcggccat gcgccggtcc agaggtgttg catgacgtcg
     2941 accgactccg ttctctcacc ggtttcggtg aacagcccgt accacgttgg cgtccgctcc
     3001 tgcttctggc cccacagaaa gacgtagttg ccgatgagct ggtccggata cggctcgatc
     3061 gccgtggcgt gcgcccggcg cagcgccttg gccttggcag agctgtccgg ctcgatcggc
     3121 gcgccccagc tggtggtcgc gacttcccag tgtccgggcg gtccccactc agtgagcacg
     3181 tacggcccct cgtatccggc cttgcgaacc cgtcgttgca ggtcgaggac gcctgcgtag
     3241 gactggatgc tgaggaagtc gatgtccggc gcgcgggcgg agatgacctc agccatgtcc
     3301 gcgctgaagc cggcgatggt ggtggtggtg ggatgattcc cgtcgatctc gtggatcatc
     3361 cgggaaatct cgttcaccgc gtcgtagacc cgggggttct tgaagtcgtg gttcagctcg
     3421 ttgccgatga tccaggccag cagggccgga tgattgcggt gcgccagcac gtccgctctc
     3481 gctcgctcga actgcgcggc caccgccgca tcatcgtcgt agtcgaagcc gtgacgttcc
     3541 ctggcaatgt ccaggcacag cgcgaccgtg aggcccagac ggtgcgcttc gtcgagcagc
     3601 tcccgcgcgg actgctcggg agttccaacc gaccaggtcc ggatggagtt gccgccggac
     3661 tcggcgagca gcgcaaggcg ctccagaccg ccgacgccct ggatccggta aggccggcca
     3721 ccgcgctcga gcgcgtagcc cctcgccgtt cgcaccacct cgactttgac cggctcatct
     3781 ggcatcgcgc ccgcttccgg cacgagcccg gcatcggcca gggacagcaa aacgagcacg
     3841 gctggaaagc gcccgagaat tgaagtcatc cgtctcccag atcgatccga aggttcgaca
     3901 ccgaaagacc cagcacgccg tcggtccaga tcaaccacgg ggtatccacc gcgcataggt
     3961 tgtccgtccc gccggcaaac gcgctcatct cgaaagcgac gtcttcccag gtccccagcg
     4021 gcagctcgtt gagcatggcc gtgatgtcga tgctcgccgt ctgaggataa ccggagtcca
     4081 tcctgagctc gaccctgcgc tggggccgca cgtccacccg cagcttgcag cgcagtcggg
     4141 cgccgcggga agcgtggtcg gagaggtcct ggcccgcgct gcgctggaag tagacctgag
     4201 cgcctccgct cccgtcccag ctgatgcggc gcgcgtcctc ctggctgtag tggtcgacgt
     4261 tggtcaccga gaccgcgccc gtgggagacc ggctggagcg gccgcgaacc ggcactttcc
     4321 aggcctggct gtcgccaatg aagaactcga acggcggcac cacgcggccg gtgaggatgc
     4381 cgatgctctc gtcggccgca aggccgagcg cctcgtcttc ctcgatgagc tcgtcaccga
     4441 gggtatcgac gtctgcggca ccgagcccga agccgtaggg gaacagcggg tcgtaatccg
     4501 catcgtggcg attcaccgtg gcctgcagcg aggagcgcgg ccaggaaaag gccagccgtc
     4561 cggtaaagtc gtgagcgacc tcgccaccca cggttcgaaa gagcacgtcg ctcacgccgg
     4621 cgccctccga gcccggaagc caggccacca cgaaggcgtc ggaagcgttc agctcggggt
     4681 tgacccagag aggccggccc gtgagaaaga cgctcaccac cgggatgccg tcgcctttca
     4741 gtttcttgag cagcgcaagc ccttcgggaa acagggcgga gaagctcag
//
LOCUS       DBNHOX010000076         2253 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1548718, whole genome shotgun sequence.
ACCESSION   DBNHOX010000076 DBNHOX010000000
VERSION     DBNHOX010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1548718"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="AC16UZ_01700"
     CDS             <1..249
                     /locus_tag="AC16UZ_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR6245422.1"
                     /translation="LLAGHAAPVASGPQTVEAEMPGKVAKLHLAPGDPVAEGQGVLVV
                     EAMKMETAVAASQDGTVAEVHVREGDAVAVGDPLVTLA"
     gene            252..1385
                     /locus_tag="AC16UZ_01705"
     CDS             252..1385
                     /locus_tag="AC16UZ_01705"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HIR6245423.1"
                     /translation="MDLLSQIWEGSGLNQLTWGQAIMIGVGLVLLYLAIARQFEPLLL
                     VTIGFGGILANIPGAGIAEGDGLLHLAYVVGIESGAFPLIIFMGVGALTDFGPLLANP
                     KTLFLGAAAQFGIFATLLGALGLSELGVFDFGLREAAAIGIIGGADGPTAIYVAGRLA
                     PELLGAIAVAAYSYMALVPLIQPPIMRALTTEAERKIEMVQLREVSRLERIVFPLLLL
                     LLVALLLPSAAPLLGMFCFGNLMRECGVVDRLSDTTQNALINIVTIFLGLAVGSKLSA
                     EQFLVPSTLGILALGMAAFALGTASGVLMGKLLNLVTREHINPLIGAAGVSAVPMAAR
                     VANRVGLEANRHNYLLMHAMGPNVAGVIGSAVAAGIMIMLVGD"
     gene            1460..1536
                     /locus_tag="AC16UZ_01710"
     tRNA            1460..1536
                     /locus_tag="AC16UZ_01710"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1494..1496,aa:Arg,seq:cct)
     gene            complement(1616..>2253)
                     /locus_tag="AC16UZ_01715"
     CDS             complement(1616..>2253)
                     /locus_tag="AC16UZ_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245424.1"
                     /translation="KTMADYDKVFNAYIEWSKENDVQTYAMRAMPLFGGPDPAAGLDF
                     QWIDMLVSPFAVSGNGWDKWMTTESGQKLDKQWRETANCRVAIHTVASMFLDREALTH
                     DSRIMSLNWCTRHDGVTWDNLMARHATMAAGRPDGAPVKAWTLLFPGLGVRNAPGEFA
                     HLLSFADTAGLMAWENQLANGGGWRQREDYQSSYASCTGSNVYRTTILHRP"
BASE COUNT          329 a          717 c          762 g          445 t
ORIGIN      
        1 ctcctggcgg gtcacgccgc cccggtggcc agcggccccc agaccgtcga ggcggagatg
       61 cccggcaagg tggccaagct gcacctggcc cccggagacc cggtggccga gggccagggc
      121 gtcctggtgg tggaggccat gaagatggag acggccgtag cggcctctca ggacggcacc
      181 gtggctgaag ttcacgtccg cgagggagat gcggttgcgg tgggtgatcc gttggtgacg
      241 ctggcctgat cgtggacctg ctgagtcaga tctgggaggg ttcgggctta aaccagctca
      301 cctggggtca ggccatcatg atcggcgtgg gcctcgtgct gctttacctg gccattgccc
      361 gtcagttcga gcctctgctg ctcgtgacca tcggtttcgg tgggattctg gccaacattc
      421 ccggcgcggg aatcgccgaa ggcgacggtc tgctgcacct ggcctacgtg gtggggatcg
      481 agtcgggcgc ttttccgctg atcatcttca tgggcgtcgg ggcgctcacc gacttcggcc
      541 cgctcctggc caacccgaaa acgctctttc tcggcgccgc ggcccagttc ggcatttttg
      601 ccaccctcct gggcgcgttg ggcctctcgg agctcggcgt cttcgacttc gggctgcgcg
      661 aggccgccgc catcgggatc atcggcggcg ccgacgggcc tacggcgata tacgtcgcgg
      721 gcaggctcgc cccggagctg ctcggagcaa tcgccgtggc cgcttactcc tacatggccc
      781 tggtgccgct gatccagccg cccatcatgc gcgccctgac caccgaggcg gagcgcaaga
      841 tcgaaatggt acagctgcgc gaggtctcca ggctcgagcg catcgttttt ccgctgctgc
      901 tgctgctgct ggtggcgctg ctgttgccat ctgccgcgcc tctgctgggg atgttctgct
      961 tcggtaacct gatgcgcgaa tgcggcgtgg tggatcggtt gtcggatacc acccagaacg
     1021 ccctgatcaa catcgtgacc atcttcctcg gccttgccgt gggctccaag ctaagcgcag
     1081 agcagttcct ggtgcccagc acgctgggca tcctggcact cggtatggcc gcgtttgcgc
     1141 tgggaaccgc gtccggcgtt ctgatgggca agctgctgaa cctggtgacc cgcgagcaca
     1201 tcaacccgct gatcggcgct gccggcgtct cggcggtccc catggcggcg cgggtcgcca
     1261 accgggtcgg gctcgaagcc aatcgccaca attatctgct catgcacgcg atgggcccga
     1321 acgtggcggg cgtgatcgga tcggccgtgg ccgctggaat catgatcatg ctggtgggtg
     1381 actgaacgcc tagtcgagcc cgtagcgttc gcagcagctg gtgcggtatc tttgctgggc
     1441 ttcgccgagc ggagcatccg ccccgatggc acagctggat agcgcggccc cctcctaagg
     1501 ggcaggtcga aggttcgaat ccttctcggg gcgccagtaa ttcgtgcttt cccgaacggg
     1561 ccgcggccat cgtcacgtcg ccacggagcg tggcgacgtg acgttagggt tggggttacg
     1621 gtctgtggag aattgtggtc cggtagacgt tggatcccgt gcagctggcg tacgagctct
     1681 ggtagtcctc gcgctggcgc cagccaccgc cattggctaa ctgattttcc caggccatca
     1741 gccccgcggt atccgcaaag ctcagcaggt gggcgaactc gccgggcgcg ttgcgaacgc
     1801 cgaggccggg gaagagcaag gtccaggcct tgaccggcgc gccgtctgga cggccggccg
     1861 ccatggtggc gtgccgggcc atgaggttgt cccaggtgac cccgtcatgt cgcgtgcacc
     1921 agttgagcga catgatgcgg ctgtcgtggg tcagggcctc gcggtccaga aacatgctgg
     1981 ccaccgtgtg aatggccacc cgacagttgg ccgtctcccg ccactgcttg tcgagctttt
     2041 gacccgactc cgtggtcatc cacttgtccc agccgtttcc gctgactgcg aagggcgaca
     2101 ccagcatgtc gatccactgg aaatccaatc cggccgcagg gtccgggccg ccgaacaggg
     2161 gcattgcgcg catggcgtag gtttgaacgt cgttctcctt cgaccattcg atgtaggcgt
     2221 tgaagacttt gtcgtagtcc gccatggtct ttc
//
LOCUS       DBNHOX010000077         3163 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1163477, whole genome shotgun sequence.
ACCESSION   DBNHOX010000077 DBNHOX010000000
VERSION     DBNHOX010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1163477"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="AC16UZ_01720"
     CDS             complement(<1..671)
                     /locus_tag="AC16UZ_01720"
                     /inference="COORDINATES: protein motif:HMM:NF018819.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter permease"
                     /protein_id="HIR6245425.1"
                     /translation="MPIELLLIVCLVVIFLVPIFFDVNVGIVAFITAFLVGALAMDLS
                     ARQVLAGFPDNMFVMLVGITLLLAIAHQNGTVDWIVAKLVRAARGRLVLLPWVLFVTA
                     LVTASLGPVAAPVLFVIGVGFVSRYGLNPLMVAAMIIHGTQSGAYSPIAPYGVVIGEL
                     AATAGIGYSPVAVYAGVVTFHVLLAAFVFLMLGGRALAGVRVEVAIGETAESERLDRQ
                     RTLTL"
     gene            complement(714..1508)
                     /locus_tag="AC16UZ_01725"
     CDS             complement(714..1508)
                     /locus_tag="AC16UZ_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6245426.1"
                     /translation="MASITEFAVRDHVAYITLNRPEALNSLNPELRWTLSRHFDEVQS
                     NDDIWLAVVTGAGERAFCAGADLKHRARERDASAEQRAEWARMMEETTPLNQRWYLAK
                     PVIARVNGFALGGGLELALSCDIIVAAEHAELGLPEPRRGLIAGGVGVHRLPRQIGLK
                     AAMGYLLTGRHMSAQRAYELGLVNQVVPGAGLDAAVDEWVRDILRCAPLAVRATKEAA
                     MRGLDLPLPQAFYTEYEWERRRRGSDDALEGPRAFAEKRPPEWRGR"
     gene            1656..2330
                     /locus_tag="AC16UZ_01730"
     CDS             1656..2330
                     /locus_tag="AC16UZ_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HIR6245427.1"
                     /translation="MANYFEGIFGRSPFKPVQEHMDLCHQAAALIEELLARAGSQSWP
                     EVEEIREQIVALERRADDRKRDIRLHLPRGFFLPVARADLLDLLARQDKIANKAKEIA
                     GLIVGRRMSFPPAIQESALELTRTSISACQRALDVVNELDELIATGFRGVEVQRVEEM
                     IDALDESESRNDDAQVRLRAELHGLERELDAVDVIFLYRIIDRIGDLADNAQSVGHRL
                     EMMLAR"
     gene            2384..>3163
                     /locus_tag="AC16UZ_01735"
     CDS             2384..>3163
                     /locus_tag="AC16UZ_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013543.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR6245428.1"
                     /translation="MDPTTLLLLLALLFGLYMAWGIGANDVANAMATSVGARAITVKQ
                     AILIAAVFEFLGAFLAGGEVTSTIRKGIIDSSALVGDPDVLILGMLAALLAAAVWLTV
                     ASRFGWPVSTTHSIVGAIVGFAIATIGMEAVHFDKVGSIAASWVVSPLLAGTLSFLLV
                     RSVQWLILDRPNPELYARRYVPAYIFLAALNISLVTLLKGLKHIGLDLTAFEAIGYSV
                     AFALVIMAGGAVMVRRIHTATATEMEDYHYANVERTFAVLMI"
BASE COUNT          562 a         1065 c         1018 g          518 t
ORIGIN      
        1 atcaaggtca gcgtgcgctg gcggtcgagc cgctctgact cagcggtctc accaatggca
       61 acctctaccc gcacgccggc cagggcccgc ccgcccagca taaggaacac gaaggcggcc
      121 agcagcacgt gaaaggtcac cacgcctgcg tagacggcca cgggcgagta gccgatccca
      181 gcggtggcag cgagctcgcc gatcaccacc ccgtagggcg cgatgggaga gtaagcgccg
      241 gactgggtgc cgtggatgat catggcggca accatcagcg ggttgagccc gtatcgggag
      301 acgaacccca cgccgatcac gaacagcacc ggcgcggcca cgggcccgag agatgcggtg
      361 accagcgcgg tgacgaagag cacccacggc agcagcacca gtcgtccccg ggcggctcgg
      421 acgagctttg cgacgatcca gtccacggtg ccgttctgat gggcgatggc gagcagcagc
      481 gtgatgccca ccagcatgac gaacatgttg tcgggaaaac cggccagcac ctgtctcgcg
      541 ctcaggtcca tggccagcgc cccgaccagg aaggcggtaa tgaaggccac gatgcccacg
      601 ttgacgtcga agaagatcgg caccaggaag atgacgacga ggcagacgat gagcaggagc
      661 tctatgggca tgacgtcaac gctgccagat cggccgcacg atggctcgca ggctcagcgc
      721 ccccgccact cgggcggacg tttctcggca aaggccctgg gcccttccag agcatcgtcg
      781 ctgccccgcc ggcgccgttc ccactcgtac tccgtataaa aggcctgggg cagcggcagg
      841 tcgaggcctc gcatggcggc ctccttggtg gcccgaacgg ccagcggcgc gcagcgcaga
      901 atatctctca cccactcgtc caccgccgcg tcgagccccg ccccaggcac cacctggttt
      961 accaggccca gctcgtaagc ccgctgggcc gacatgtgcc ggcccgtgag gaggtaaccc
     1021 atggccgctt tcaggccgat ctgccgcggc agccgatgca cgcccacgcc gccggcgatc
     1081 agtccccgtc ggggctccgg caggccgagc tcggcgtgct cggccgcaac gatgatgtcg
     1141 caggacagcg ccagctccag cccgccgccc agcgcgaagc cgttgacccg cgcgatgacg
     1201 ggcttggcca ggtaccagcg ctgattgagg ggggtcgttt cttccatcat gcgcgcccat
     1261 tcggcgcgct gctcggcgct ggcgtcgcgc tcgcgggcgc ggtgcttgag gtcggcgccg
     1321 gcgcagaacg cccgctcgcc ggctccggtc accaccgcca gccagatgtc gtcattgctc
     1381 tgcacctcgt cgaagtgtcg cgagagcgtc cagcgcagct cagggttcag ggagttgagc
     1441 gcctcggggc ggttcaaggt aatgtacgcc acgtgatcgc gcaccgcgaa ctcggtaatc
     1501 gatgccatcg tccccttccc cgaactcacc cagacgagta accggtcaat ctaacgtgga
     1561 aatcagagcc cggcaatcgg caagaagacc gaatgtccac ttccctttgc ccagccacgt
     1621 gggttagttt tcgccgcggg aagggagaca tatccgtggc gaactacttc gagggcatct
     1681 tcggccgctc gcctttcaag cccgtacagg aacacatgga tctgtgccat caggccgcgg
     1741 cgctcatcga agagctgctg gccagggcgg gatcccagtc ctggcccgag gtggaggaga
     1801 tacgcgagca gatcgtcgcc ctggaacgcc gggccgacga ccgcaagcgg gacatccgcc
     1861 tgcacctgcc ccgaggcttc ttcctgccgg ttgcgcgcgc cgacctgctg gacctgctgg
     1921 cgcgccagga caagatcgcc aacaaggcca aggagattgc cgggctcatc gtggggcggc
     1981 gcatgtcttt tccgccggcc attcaggaat cggctctgga gcttacccga acgtccatct
     2041 cggcctgcca acgtgcgctg gacgtggtca acgagctcga cgagctcatc gcgacgggct
     2101 tcagaggcgt agaggtgcag cgggtggaag agatgatcga cgccctcgac gaaagtgaat
     2161 cccgcaacga tgacgcccag gtccgcctcc gagcggaact gcatggtctg gagcgggaac
     2221 tcgatgccgt ggacgtgata tttctgtatc gcatcatcga tcgcatcggt gatctggctg
     2281 acaacgccca gagcgtcggc cacaggctgg agatgatgct ggcgcgttga gggcgcaagc
     2341 ttcctggcgc tgagccgaca gaaaaaccgg gaaagggatg gcgatggacc cgacgacact
     2401 gctactgctg ctcgcgctgc tgttcggcct ctacatggcc tggggcatcg gcgccaacga
     2461 cgtcgccaat gccatggcca cctccgtcgg cgcccgggcc attaccgtga agcaggcgat
     2521 tctgatcgcc gcggtgttcg agttcctggg cgcttttctg gccggcggag aagtcacgtc
     2581 caccatccgt aaaggcatca tcgactcgag tgctctggtg ggcgatccgg atgttctgat
     2641 actggggatg ctcgccgcgc tgctcgccgc agcggtgtgg ctcacggtgg cctcaaggtt
     2701 cggctggccg gtctccacca cccactccat cgtcggcgcc atcgtcggct ttgccatagc
     2761 caccatcggg atggaggccg ttcacttcga caaggtcggc tccatcgcgg ccagctgggt
     2821 cgtctccccg ctgctggcgg gcacgctctc gtttctgctg gtccggtccg tgcagtggct
     2881 gatcctggac cgcccgaacc ccgagctgta cgcccggcgc tacgttcccg cctacatctt
     2941 cctcgccgcg ctgaacatct cgctggtgac gctgctcaaa gggctcaaac acatcggcct
     3001 ggatctgacc gctttcgagg ccatcggcta ttccgtggcc tttgccctgg tcatcatggc
     3061 cggcggcgcc gtgatggtgc gacgcatcca caccgcaacg gccacggaga tggaggacta
     3121 ccactacgcc aacgtggagc gcacgtttgc cgtgctgatg atc
//
LOCUS       DBNHOX010000078         3776 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_456408, whole genome shotgun sequence.
ACCESSION   DBNHOX010000078 DBNHOX010000000
VERSION     DBNHOX010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_456408"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="AC16UZ_01740"
     CDS             complement(<1..255)
                     /locus_tag="AC16UZ_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245429.1"
                     /translation="MGVVFAGVVSHAPGITGRAERADPALRDALYENFYRLGRELKES
                     RPDALIVIAAEHFANFFMDNMPAYCMGMADYYEGPIEDEQW"
     gene            complement(255..569)
                     /locus_tag="AC16UZ_01745"
     CDS             complement(255..569)
                     /locus_tag="AC16UZ_01745"
                     /inference="COORDINATES: protein motif:HMM:NF019366.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245430.1"
                     /translation="MSLYYVQKLLYQLNRDPHARRRYEEDFDELLAEYDLDAEELHAI
                     REPDIGLLYVMGVNGQILMHYAALRGYAWDEYIKAMRDGLAEHGDVRAGLYVTTDGRG
                     AV"
     gene            complement(577..2100)
                     /gene="hpaE"
                     /locus_tag="AC16UZ_01750"
     CDS             complement(577..2100)
                     /gene="hpaE"
                     /locus_tag="AC16UZ_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010324201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HIR6245431.1"
                     /translation="MSQLLHENLAKLDLHLARFRNRTLGHLIDGEEAEGVSGETFTNT
                     TPVDDSPVNEVARGAEEDVTRATRAAVRAFGEWRDMPGARRRGLLHGIADAIEARAEE
                     IALVESYDTGQPLRFMSSAALRGAENFRFYADRAPEARNGLSTPADQHVNYSVRQPIG
                     PVGVITPWNTPFMLSTWKIAPALAAGCTVVHKPAEWSPLSASILAEICRDAGLPAGVL
                     NTVHGLGEEAGVALTEQPDIRAIAFVGESQTGRAIMSQGASTLKRVHFELGGKNPVIV
                     FDDADLERALDACTFMIYSLNGERCTSSSRLLVQATIYDEFCARVAERAGQIRVGHPL
                     DPATEVGPLIHPRHLEKVLGYCALAQTEGATVAVGGGPPGDAAAGNYVAPTLFTHARN
                     DMRIAQEEIFGPVLTAIPFDDEAEAVELANQVEFGLAGYVWTGDVGRSHRLARDLDAG
                     MIWVNSENNRHLPTPFGGMKASGIGRDGGDYSFDFYMETKNVCISLGKHRVPSLGKG"
     gene            complement(2097..2528)
                     /gene="hpaR"
                     /locus_tag="AC16UZ_01755"
     CDS             complement(2097..2528)
                     /gene="hpaR"
                     /locus_tag="AC16UZ_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HIR6245432.1"
                     /translation="MLLLRARETSMAYFRPSLQERGLTEQQWRVLRALQESGELSASG
                     LSHACVILAPSMTRILRRLAEEGLVSSKRSRRDGRELTVRLTRAGERLIDEIAPISER
                     QYALIRERLQPEKLEQLHSLLHDFVELDGEHLKGAASGETQ"
     gene            complement(2651..>3776)
                     /locus_tag="AC16UZ_01760"
     CDS             complement(2651..>3776)
                     /locus_tag="AC16UZ_01760"
                     /inference="COORDINATES: protein motif:HMM:NF015214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6245433.1"
                     /translation="SVMAFAAASEFLAGEGGRPEYAENMCRYYEKARVEDLVLTHTLV
                     NPQFNAALAAAGRSSPEVALQIVGENDAGVVVNGARLLATLGPHADEIEVFPSTLLKA
                     SPENVPFAIAFTIPIETRGLRLICRDTYDAGKSHFDAPLASRFEEMDAVVIFDNVLVP
                     WERVFMYQAPLLCNRAFAETNAVVHMMHQVACGKLAKAEFIVGLLCAMAKASGKDKDA
                     NVKGQIAEAMWIVETVRAFLFSAEQQAEPDRWGLWVPKRLPLDTSRNLFPRLYPKLVE
                     TVHMLGSSSLMATPCEADFENAIAGDVEQYFQLTNLESRDRVALFRLAHDVAVSGFGN
                     RQVLYERFFFGPQPVMASIYYDLYDKDSVMDRVTEMLNGC"
BASE COUNT          660 a         1262 c         1174 g          680 t
ORIGIN      
        1 ccactgctcg tcttcgatgg gcccttcgta gtaatcggcc attcccatgc agtaggcggg
       61 catgttgtcc atgaagaagt tggcgaagtg ctcggcggcg atgacgatca gcgcatccgg
      121 gcgcgactcc ttcagctcgc gccccaggcg gtagaaattt tcgtacaggg cgtcccggag
      181 cgcgggatcg gcgcgctctg cgcgccccgt gatccccgga gcgtggctga ccacgccggc
      241 aaacacgacc cccatcagac ggcccccctg ccgtccgtgg tcacgtacag cccggcccgg
      301 acgtctccgt gctcggcaag gccgtcacgc atggccttga tgtactcgtc ccacgcgtag
      361 ccgcgcagcg cagcgtagtg catgaggatc tggccgttca cgcccatcac gtagagaagc
      421 ccgatgtcgg gctcccgaat ggcgtggagc tcctccgcgt cgaggtcgta ctcggccagc
      481 agctcgtcga aatcctcctc gtagcgtcgg cgggcgtgag ggtcccgatt cagctggtag
      541 agaagcttct gcacgtagta gagactcacg gtagcctcac cccttgccca ggctgggcac
      601 acggtgcttg ccgagggata tgcacacgtt cttggtttcc atgtagaaat caaagctgta
      661 gtcaccgccg tcccggccga tgccgcttgc cttcatgccg ccgaacgggg tcggcagatg
      721 acggttgttt tccgagttga cccagatcat gcccgcatcc aggtcgcgcg caagccggtg
      781 cgatctgccc acgtcccccg tccagacgta accggccagg ccgaactcca cctggttggc
      841 aagctcaacg gcctcggctt cgtcgtcgaa agggatcgcc gtcagcacgg gaccgaatat
      901 ctcctcctgg gcgatacgca tgtcgttgcg cgcgtgggta aagagcgtgg gcgccacgta
      961 gttcccggcg gccgcgtccc ccggagggcc gcccccgacc gccacggtag ccccttcggt
     1021 ctgcgccagc gcgcagtacc cgagcacctt ttcgaggtgg cgcggatgaa tcagcggtcc
     1081 cacctcggtg gcgggatcca gcgggtggcc cacacggatc tgcccggctc gctcagccac
     1141 ccgggcgcag aactcatcgt agatcgtcgc ctgcacgagc agtcggctcg acgaggtgca
     1201 ccgctcgccg ttgaggctgt agatcatgaa cgtgcaggca tccagcgcgc gttcgagatc
     1261 ggcgtcgtcg aacacgatga ccggattctt cccgccgagc tcgaagtgca cgcgcttgag
     1321 cgtgctcgcg ccctggctca tgatcgcgcg gcccgtttgg gactcgccca cgaaagcgat
     1381 ggctcggatg tccggctgct ccgtgagcgc cacgccagcc tcctcgccta gcccatgaac
     1441 cgtgttgagc acgccggcag gcagcccggc atctcgacag atctccgcga gtatgctggc
     1501 cgagaggggg ctccactctg ccggcttgtg caccaccgtg cagccggcgg ccagagccgg
     1561 agcgatcttc caggtggaga gcatgaacgg cgtgttccaa ggggtgatga cgccgaccgg
     1621 cccgatcggt tggcggacac tgtagttcac atgctgatcg gcgggagtgg agaggccgtt
     1681 gcgggcttcc ggcgctcggt cggcataaaa ccggaagttt tcggctccgc gcagcgccgc
     1741 gctgctcatg aaacgcagcg gctggccggt gtcgtacgac tccaccaggg caatctcctc
     1801 agcccgcgcc tcgatggcat cggcgatccc gtggagcagg ccgcgccggc gggcacccgg
     1861 catgtcacgc cactcgccaa acgcccttac ggctgcccgc gtcgctctgg ttacgtcttc
     1921 ctcggcaccc cgggccacct cgttcaccgg agagtcgtcc accggggtag tgttcgtaaa
     1981 cgtctcgccc gagacgcctt cggcttcctc cccgtcgatg aggtgcccca gcgttcggtt
     2041 gcggaaacgc gccaggtgca ggtcgagctt tgccaggttt tcgtgaagca gttggctcat
     2101 tgggtctctc ccgatgcggc ccctttcagg tgctcgccat ccagttccac gaagtcgtgc
     2161 agcaggctgt ggagctgctc cagcttttcc ggttgcagcc gttcgcggat gagcgcgtac
     2221 tgacgctcgc tgatgggcgc gatctcgtcg atcagacgct cgcccgcgcg ggtcaacctc
     2281 accgtgagct ccctgccgtc gcggcgcgac cgcttcgacg aaacgagccc ctcctcggca
     2341 agccgacgca gtatccgcgt catgctgggc gccagaatca cgcacgcgtg tgaaagcccc
     2401 gacgccgaca gctcgccaga ttcctgcaac gcccgaagca cacgccattg ctgctcggtg
     2461 agcccccgct cctgcaggga aggccggaaa taggccatag aggtctcgcg ggcccggagc
     2521 agaagcatgg gcagagactc actgaatttg cggggttgcg ccacgcaagg ctccggcctt
     2581 ccaagatgag cgattattta acatgttaat tccctgtgtt gcatccgcaa tgccactgga
     2641 acccgtcgtc tcaacagcca ttgagcattt ccgtgacgcg atccatcacg ctgtccttat
     2701 cgtaaagatc gtagtagatc gaggccatga ccggctgggg accgaagaaa aagcgctcgt
     2761 agagcacctg acggttgccg aatcctgaga ccgcgacgtc gtgcgccagc cggaacaggg
     2821 caacgcggtc cctgctctcc agattggtga gctgaaagta ttgctccacg tccccggcaa
     2881 tggcgttttc gaaatcggcc tcgcagggcg tggccatgag agaactagag ccgagcatgt
     2941 gcaccgtttc cacgagcttc ggataaagcc tcgggaaaag attacgggac gtgtccaggg
     3001 gcaggcgctt gggtacccac aggccccagc gatcaggttc tgcctgctgc tcggcgctga
     3061 acagaaaggc ccggacggtt tcgactatcc acatcgcttc cgcgatctgg cctttgacgt
     3121 tggcatcctt gtctttgccc gaggccttgg ccatggcgca gagcagaccc acgatgaact
     3181 ccgccttggc cagcttcccg caggccacct gatgcatcat gtgcacgacg gcgttggtct
     3241 ccgcaaacgc gcggttgcac agcagcggcg cctggtacat gaacacacgc tcccagggca
     3301 ccaacacgtt gtcgaagatg accaccgcgt ccatttcctc gaatcgggat gccagaggcg
     3361 catcgaaatg gctcttgccc gcgtcgtagg tgtcccgaca gatgagccgc aggcccctgg
     3421 tttcgatggg gatggtgaaa gcgatggcaa agggcacgtt ctccggcgag gccttcagga
     3481 gggtggacgg aaacacctcg atctcgtccg catgggggcc cagcgtggca agcaaccgcg
     3541 cgccgttgac gaccacgccg gcatcgttct cgcccacgat ctgcagggcc acctcgggac
     3601 tcgatcgacc cgccgccgca agcgccgcgt tgaactgcgg attgaccagc gtgtgggtga
     3661 gcaccaggtc ctccacacgg gccttttcgt agtagcgaca catgttctcc gcgtactccg
     3721 gtcgcccgcc ctccccggcg agaaattccg acgccgcggc aaacgccatc accgag
//
LOCUS       DBNHOX010000079         5491 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1677739, whole genome shotgun sequence.
ACCESSION   DBNHOX010000079 DBNHOX010000000
VERSION     DBNHOX010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1677739"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="AC16UZ_01765"
     CDS             <1..411
                     /locus_tag="AC16UZ_01765"
                     /inference="COORDINATES: protein motif:HMM:NF022444.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ssDNA-binding protein"
                     /protein_id="HIR6245434.1"
                     /translation="LRDGDKYLDAEGEVRPDFAGKKYLAAKNDAKPVVIAHAFHNGKP
                     VVLSADGGTFQDGVPVAVPFEAKAPYRGCYVNIKVSIDAFTSKNAEVGRMVAAKILAV
                     QFVKDGDAFGSAPPTADGFADLGASTTYDPLGAF"
     gene            462..773
                     /locus_tag="AC16UZ_01770"
     CDS             462..773
                     /locus_tag="AC16UZ_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245435.1"
                     /translation="MSLTFEVASNISVPKRTGRPGGRPLAYPFEAMAAGDCLLTPAPQ
                     DPTPENVSKLMKRVRSAAQAWKKRAGATCGFVVKVVTEGEHAGKVGCWKVAGRTKKAA
                     E"
     gene            770..1141
                     /locus_tag="AC16UZ_01775"
     CDS             770..1141
                     /locus_tag="AC16UZ_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245436.1"
                     /translation="MKLSTMFAHGCTLGCCMSVAVGEVVKLAGDCSDFSSAIRHVLLA
                     TVGAVGALNAYRTVRTGALRDACGMKTVTITQEQLAAAITEWEQTSRDEQWPATPEDS
                     PAKVGEDSAAYLFPLLEQKTA"
     gene            1646..4720
                     /locus_tag="AC16UZ_01780"
     CDS             1646..4720
                     /locus_tag="AC16UZ_01780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase family A protein"
                     /protein_id="HIR6245437.1"
                     /translation="MKLFLDFESYSELDLKEVGTYVYAANAEILLAAFAIDDGPVEVW
                     DATGEPMPVKLRDALLIVQSDPTSELIAHNGAFDKALLNTCLPALAPPLERWRCSMAQ
                     ALSHALPASLGELCKVLKVPEDQAKLKEERKLVQLFCKPQAANRKVRRATRETHPAEW
                     ERFKKYAANDVVAMRECVARMPRWNWDASAIAEWHCDQRINERGFQVDTELTAAGALA
                     ATTEKARIGVRFRELTRGLVDRPSLRADFLKFLNSTYGLGIDNTRSETFQHLLKTRDL
                     PADCAELLRLAIASNKTSTAKYAALAPATSNDGRFRGGLQFAGASRTRRWAGRIFQPQ
                     NLPSRGLPKAEQIAEYIDALKLGVHTDFFEPGELMRFGAAALRGVVIPRPGMRLAVAD
                     LSNIEGRMLAWLAGEQWKLQAFRDFDAGTGPDLYNITAASIIGGDPYRVSKTNRNVFG
                     KVPDLASGYQGGVSGYQTFARAYGVKMADYWTTIRENIDARHISKAHENLEKWGHKQL
                     ADLEIDASEWLASETCKLAWRARHPATERFWYALGGAAKDAIREWGQVFTAGQWLKVR
                     GVTHRGQRWLVIALPSGRYLTYFDPQLTDDGSITYMGDAAEQGSTNRMWVRVHTHGGK
                     LTGNCFAASTQVLTPTGTKSICDVTSADWLWDGAEWVQSDGVVNQGKKEVGTWIGLSV
                     TRNHLIFDGSTWRPAEKMDKATSLNALSWAQSSARSLSFQQPPVRKAVVPSAGASAAE
                     PRTSACGNSPEDRASGAGRAQGPQWAPVSGKTWVSGLPKKCGTSGASGTSQWCAGATS
                     QPTPRTATMVPEAFASMNPGSQTDWPFSSTHGRYPGGTSWVSNLTEWTTTRATSPETS
                     DWSHSETMPTIGAVPFDSTSEDRTTRLQIFANSSYRTGTPTTPFGTTKASGETWSGSW
                     HTTTRQRLVFDILNCGPRNRFTVLSDHGPVVVHNCCQTIARDQLMSALPVAEAKGFTP
                     VLTVHDEVIAEFPADGSLSYRDLVSIMATNQPWNPGLPLAAAGFDTMRYCKD"
     gene            4880..5326
                     /locus_tag="AC16UZ_01785"
     CDS             4880..5326
                     /locus_tag="AC16UZ_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK family nuclease"
                     /protein_id="HIR6245438.1"
                     /translation="MNCTESRPERARGCADRNTHYIRLQKPAETPTQPASATRPPTRE
                     SQIETYLKQQVKIRNGEIRKVTWVGQSKAPDRLVMLPNRADPSPGAPGHTLWVELKAP
                     GKGNTFPADAHERAQAREHARMRAVGQRVFVIDSYEGVDRLLNQYR"
     gene            5336..5473
                     /locus_tag="AC16UZ_01790"
     CDS             5336..5473
                     /locus_tag="AC16UZ_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245439.1"
                     /translation="MNKALLARIEQIFFAKLTAKTGWGRNEVMSLYKDAVTEAVLETL
                     N"
BASE COUNT         1111 a         1779 c         1740 g          861 t
ORIGIN      
        1 ctgcgcgacg gcgacaagta cctggacgcc gaaggcgagg tacgccccga cttcgccggc
       61 aagaagtacc tggctgccaa gaacgacgcg aagccggtgg tgatcgccca tgcgttccac
      121 aacggcaagc cggtggtcct gagcgccgac ggtggcacct tccaggacgg cgtgccggtc
      181 gccgtgccgt tcgaggccaa ggccccgtac cgcggctgct acgtgaacat caaggtctcg
      241 atcgacgcgt tcacctcgaa gaacgccgaa gtcggccgca tggtcgccgc caagatcctg
      301 gccgtgcagt tcgtcaagga cggcgacgcc ttcggcagcg ccccgccgac cgcggacggc
      361 ttcgccgacc tcggcgccag caccacctac gacccgctgg gcgcgttctg attcacgggg
      421 ctccggcccc ttcctgccct ctcctttcct gaaagacccc catgtccctg accttcgaag
      481 tcgcatccaa catttccgtc ccgaagcgca ccggccgccc cggtggccgc ccgctggcct
      541 acccgttcga ggccatggcc gccggtgact gcctgctgac ccccgcgccg caagacccga
      601 cgcccgagaa cgtcagcaag ctgatgaagc gcgtgcgcag cgccgcccag gcgtggaaga
      661 agcgcgctgg tgccacctgc ggcttcgtgg tcaaggtcgt caccgaaggc gagcacgccg
      721 gcaaggtggg ctgctggaag gtcgcgggcc gcaccaagaa ggccgcggaa tgaaactcag
      781 cacgatgttc gcgcacggtt gcacccttgg gtgctgcatg tctgttgcgg taggcgaggt
      841 cgtcaaactg gcaggggatt gcagcgactt ctcgtctgcc attcggcatg tcctcctcgc
      901 aacggtcggc gcggttggtg cactgaatgc ctaccgcacg gtgaggacag gcgctcttcg
      961 ggacgcctgc ggaatgaaga ccgtcaccat cacccaggag caactggccg cggccatcac
     1021 cgagtgggag cagacctccc gcgacgagca gtggccggcc acgccggaag actctccggc
     1081 caaggtcggt gaggacagcg cggcctacct ctttccgctg ctggaacaga agacagcatg
     1141 acccctcccc gcccaggaac tcctgtctgc tgggtgcggc gcaacggccg cctggtctgt
     1201 ggccgcgtcg tcggcccgct gaaccacggc ccgcgcggcg cgttcatccc ggtgcaggag
     1261 ttcgtgggcg gcaggccagc agggaaccgc cagatggtgc gacccagcgt cttgcgactc
     1321 gacaagtagt cgccgcctcg tccacgtcac ggacggtcgt cagggaagcg gcggtgtgga
     1381 aggacacgca gcgtcggcca agcgcgatgc agacacccgg agaggcacgg ccgggcgggc
     1441 gacaaggggc tgggggtacc cggccgaccc gtgagtagct gcagccggtg tcgagcccgg
     1501 cccgcttccc tgacgatccg cgagcaataa attcgtctct tcggagacgt ggattgaaac
     1561 atgtctgatc ggcaaacccg ctgcttcatg cgagtcgcgg atcgtcaacc tcaagtagca
     1621 agcaacgagt aactgaaagt atgcgatgaa gcttttccta gatttcgagt cctactctga
     1681 actcgacctg aaagaagtag gaacctacgt gtacgccgcc aacgcagaga tactgctggc
     1741 ggcgtttgcc attgacgacg ggcctgtgga ggtctgggac gcaaccggcg agcctatgcc
     1801 ggtcaagctc cgagatgcac tgttgatagt gcaatcagac ccaacgtcgg agttaatagc
     1861 gcacaacgga gccttcgaca aagctcttct caacacctgc ctccctgcgc tggcgccgcc
     1921 gctcgaacgc tggcggtgca gcatggccca ggccctcagc cacgcgctgc cagcgagcct
     1981 tggcgagttg tgcaaggtgc tcaaggtgcc ggaggaccag gccaagctca aggaagagcg
     2041 caagctggtc cagctcttct gcaagccgca ggccgcgaac cgcaaggtcc ggcgcgcgac
     2101 ccgcgagacg cacccggccg agtgggaacg cttcaagaag tacgcggcca acgacgtggt
     2161 cgcgatgcgc gaatgcgtgg cccggatgcc gcgctggaat tgggacgcct ctgccatcgc
     2221 cgagtggcac tgcgaccagc ggatcaacga gcgcggcttc caggtggaca ccgaactcac
     2281 tgccgcgggc gccctggccg cgaccacgga gaaggcccgc atcggcgtgc gattccgcga
     2341 gttgacccgc ggcctcgtgg accgcccctc gctgcgcgcg gacttcctga agttcctgaa
     2401 ttcgacctac ggcctcggga tcgacaacac gcggtcggag acgttccagc acctgctcaa
     2461 gacccgcgac ctgccggccg actgcgcaga gctgttgcgc ctggcgatcg cctcgaacaa
     2521 gaccagcacc gcgaagtacg ctgcgctcgc gccggccacc tcgaacgacg ggcggttccg
     2581 cggcggcctg cagttcgccg gtgcatcgcg aacgcgcagg tgggcgggcc ggatcttcca
     2641 accccagaac ctgccgagcc gcgggctgcc gaaggccgag cagatcgccg agtacatcga
     2701 cgcgctgaag ctgggcgtcc acaccgactt cttcgagccg ggcgaactca tgcggttcgg
     2761 cgcggcagca ctccgaggcg tggtgatccc gcggcctggg atgcgcctgg cagtcgccga
     2821 cttgtcgaac atcgagggcc gcatgctggc gtggctggca ggcgagcagt ggaagctgca
     2881 ggcgttccgc gacttcgacg ccggtaccgg cccggacctc tacaacatca ccgccgccag
     2941 catcatagga ggcgatccct accgagttag taagactaac cgcaacgtct ttgggaaggt
     3001 tccggatctc gcatccggct atcaaggcgg agtttctggg taccagacgt tcgcacgagc
     3061 ctatggcgtg aagatggcgg actactggac gacgatccgc gagaacatcg acgcgcgcca
     3121 catctcgaag gcgcacgaga acctggagaa gtggggccac aagcaactcg ctgacctgga
     3181 gatcgacgcg agcgagtggc tggcgagcga gacctgcaag ctggcatggc gcgcacggca
     3241 cccggccacg gagcggttct ggtacgccct cggcggcgcg gcgaaggacg cgatccgcga
     3301 gtggggccag gtgttcaccg ccggccagtg gttgaaggtc cgaggggtca cgcaccgcgg
     3361 gcagcggtgg ctggtgatcg ccctccccag tggccgctac ctgacctact ttgacccgca
     3421 gttaacggat gacggttcaa tcacctacat gggcgatgcg gccgaacagg gatctacaaa
     3481 ccgcatgtgg gtccgagtac acactcacgg gggcaagctc acagggaatt gctttgcggc
     3541 gagcacgcaa gtgctaaccc ccactggtac aaagagcatt tgcgacgtaa catcagcaga
     3601 ttggctgtgg gatggtgccg aatgggtgca gtccgacggt gttgtaaacc aaggaaagaa
     3661 agaagtagga acatggatcg gactatcggt aacgcggaac cacttgatct tcgatgggag
     3721 tacgtggaga cccgcggaga aaatggacaa ggctacttcg ctaaacgccc tatcgtgggc
     3781 gcaatcttcg gcccgctcac tgtcctttca gcaaccgccg gtacgaaagg cggtcgtgcc
     3841 ttctgccggt gcgagtgcgg cggaaccaag gacatctgcg tgcggcaact ctcccgagga
     3901 cagagcctcc ggtgccgggc gtgcgcaggg gccgcagtgg gcgcccgttt ccggaaagac
     3961 ctgggtcagt ggccttccga agaagtgcgg aacatctggc gcgagcggca caagtcaatg
     4021 gtgcgccggt gccacgagcc aaccgacgcc gcgtaccgca actatggtgc ccgaggcatt
     4081 cgcgtctatg aaccctggat cacagaccga ctggcctttt tcgagtacgc acggacgcta
     4141 ccccggtggg acgagttggg tctcgaactt gaccgaatgg acaacgacaa gggctacgag
     4201 cccggaaacc tccgattggt cacacagcga gacaatgcca acaataggcg cagttccgtt
     4261 cgactcaact tcagaggatc gaactacacg attgcagatt tttgcaaaca gttcctaccg
     4321 aactggaaca cccacaacac ccttcggtac caccaaggcg tcgggcgaga cctggagtgg
     4381 atcgtggcac accacgacaa gacaacgact ggtcttcgac atcctgaact gcggcccccg
     4441 gaatcgcttc acggtgctgt ctgaccacgg gccagtcgtc gtccacaact gttgccaaac
     4501 aattgcacgc gatcaactga tgagtgcttt gccggttgca gaggcgaagg gatttactcc
     4561 ggtgctcacc gtccacgacg aagtgatcgc ggagttcccc gccgacggct cgctgtccta
     4621 ccgcgacctc gtctcgatca tggcgacgaa ccagccgtgg aaccccggcc tccccctggc
     4681 cgcggctggg ttcgacacga tgcgatattg taaagactga ttgcgcacct gctaactcgc
     4741 tgctacactg aaatcctcag caacagaaag cgagttagcg atgagcaaga cgtttgagca
     4801 taaaggcgag tggtttgaag aagtacccga cggcccttta aggggtcgga gtgcgtgcca
     4861 cgagtgtgcg ttcggcccga tgaactgcac cgagagccgg ccggagcgag cgcggggttg
     4921 cgcggatcga aacacccatt acatccgcct ccagaagccc gccgagaccc ctacacaacc
     4981 ggccagcgcc acgcgacccc cgacccgcga gtcgcagatc gagacctacc tcaagcagca
     5041 ggtcaagatc cgcaacgggg agatccggaa ggtcacgtgg gtcgggcaga gcaaggcgcc
     5101 cgaccgcctg gtgatgctgc ccaaccgcgc cgacccgtcg cctggcgccc ccggccacac
     5161 gctctgggtc gagttgaagg cgcccggcaa gggcaacacc ttccccgccg acgcccacga
     5221 gcgagcccaa gcccgcgagc acgcacgcat gcgcgccgtc ggccagcggg tgttcgtgat
     5281 cgacagctac gaaggcgtcg atcgtctgct gaatcaatac cgttgaaagt aagtcatgaa
     5341 caaagccctt ctcgctcgca tcgagcaaat cttcttcgcc aagctgaccg ccaagaccgg
     5401 atggggtcgc aacgaagtga tgtcgctcta caaggacgcc gtcacggaag cagttctcga
     5461 aacgctcaac tgaaagtaag tcatgcaagt c
//
LOCUS       DBNHOX010000080         7419 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_391967, whole genome shotgun sequence.
ACCESSION   DBNHOX010000080 DBNHOX010000000
VERSION     DBNHOX010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7419
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_391967"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1040)
                     /locus_tag="AC16UZ_01795"
     CDS             complement(114..1040)
                     /locus_tag="AC16UZ_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6245440.1"
                     /translation="MRYLSLSITGLALLAAIGTAHAADAPFCGDEGVWVQILGAGGPE
                     LDDGQAGPSYLIWRDNHARLLVDAAPGSSAAFDRAGAEFEDLDAIAFTHLHADHSADF
                     PAFIKGSYFAERDRPLTVLGPSGEGVFPDTVTFVERMIGKDGAYPYLADFLTYGSAGG
                     YKVNARNVPSTGQRRWSRFGTEHIRLSAIPVHHDIVPAVAWRVEVGDKTIVFTGDFSN
                     QKNVIPSFAKDADALVVSHAIPETARGAARELHAIPSQLGQIAAQANARMLILGHRMN
                     RTRGRETQSREAIDKHYQGSVIFANDMECWGL"
     gene            complement(1095..3020)
                     /locus_tag="AC16UZ_01800"
     CDS             complement(1095..3020)
                     /locus_tag="AC16UZ_01800"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HIR6245441.1"
                     /translation="MDYTELGFIRVAAVAPELSIAEPATNAEKIVEHLTALEGRAVTI
                     ALFPELALTGYSCEDLFFSEQLHAAARSALLMLARSAGEAVCVVGTPYLLEDGRLLNC
                     AAVIADGRVRAMIPKIAQPNYGEFYERRWFVSGAGVDVSVDDPELGSFQVATNQLMRI
                     GNMRFAVEICEDLWAPEPPGIRHSLAGAELVVNLSASNELVAKADYRRDLVHMASARG
                     ICGYVYASSGPLESTKDLVFGGHLIACENGQLLGESPRYAFEPQTLITDIDIAKLRHD
                     RMQNTTFTNAPRPAPYHVVDVLQRPRPIAALERVYTAHPFVPDDEAQFEARAAEILDI
                     QTTGLARRCLAANTETLVIGLSGGLDSTLAFLVCLDTLDKIGRPRQSIQAITMPGPGT
                     TEHTLESARGLCAAAKVALKEIPIHEAVAQHLDALEHTGRDDLVFENTQARERTQILF
                     DYANKVSGIVVGTGDLSELALGWCTYNADHMASYNVNASVPKTMMAYLVRWYAKHRAG
                     EALAGVLQRVLQTPISPELIAPDTAGSISQETEAIIGPYELHDFFLFHFLRNGAGPAK
                     IFHLARLAFTGSYADDEIKRWLRLFFQRFFTQQFKRTTLPPGPKVGSVSLSPRGDWRM
                     PDEAGVQRLLADIDDLG"
     gene            3079..4200
                     /locus_tag="AC16UZ_01805"
     CDS             3079..4200
                     /locus_tag="AC16UZ_01805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6245442.1"
                     /translation="MAILGMLMNFEFNEDQNLLREQARGFLADNCSPAVVRAVLEGDA
                     SYDADLWRKIVEMGYTATVIPEEFGGLGLSYLELSVIAEELGRVAAPVPFSSSVYLAT
                     EAILLAGSREQKETWLPRLAAGEVIGTFAFGESHGRPTAENLTARVTDGKLSGTKLPV
                     PDGGAADVAVVVASNGLYLVDLAQDGVAVTDVPTLDPTRGHSRIEFAGVQAEPLGDPA
                     DGWSLTEKVLDRAAVLFAWEQVGGSEASLEMAKEYALGRYAFGRPIASYQAIKHKLAN
                     MYVKNTLAKSNCYYGAWALNTDASELTLAAATARVSATQAYYFASKENIQTHGGMGYT
                     WEFDCQFYYRRAKLLSVNIGSEAHWQDRLITAVEQSNAA"
     gene            4210..5424
                     /locus_tag="AC16UZ_01810"
     CDS             4210..5424
                     /locus_tag="AC16UZ_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007943237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6245443.1"
                     /translation="MDFNDTAEEAKFREEVRGWLEVNVPTAEELAGLNYIEAAKLWQK
                     RKYDAGWACIRWPEEYGGRGASAIDQVIWNQEEAKYPDLPQGIFGIGQGMAAPTLMTW
                     ADDEAKARYLPRLASGEDIWCQLFSEPAGGSDLAALRTKAERDGDDWVINGQKIWTSG
                     AHYSDYGILVVRTDPTVPKHKGLSYFYLDMKSPGIEIKPIKQISGDSNFNEVYFTDVR
                     IPDSQRLGAVGQGWQVSLTTLMNERASIGGGGTAVGFDSVFKLAQRVNIGDRPAIEDS
                     QVRAKLADWFCQEAGLRFTGYRSMTALSRGEIPGPENSIGKLVGAPKTQDMASFAIDL
                     MEMTGAIWDKEFAEEAGLFQGTYMAIPGLRIAGGTDEIMANIIAERVLGLPQEPRMDK
                     GIPFNEVPTGSG"
     gene            complement(5426..6304)
                     /locus_tag="AC16UZ_01815"
     CDS             complement(5426..6304)
                     /locus_tag="AC16UZ_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6245444.1"
                     /translation="MTSPARDAPITGGCLCGAVRFAARGRPLWTAHCHCESCRRHTSS
                     LLATYAGFPSEDVEFSGDQPALIRSSPQAERGHCGRCGSPLCYRSARWPGETHLFLPS
                     FDDPARFEVEAHVMFDERVAGFDVYDDLPRYATGSPTPIAWGPRPAARVLFLCTGNSA
                     RSIIAQTIANDLRATVNGERVRAHSAGSRPVGEVNSGAMEVLRAHGHDVRGLRSKSWD
                     EYTGDAAPALRWVITLCDSAAGETCPAFASRAGVPVERLHWGLPDPASGAASFEAVYQ
                     TLAARIDELIAGAAPA"
     gene            complement(6301..6594)
                     /locus_tag="AC16UZ_01820"
     CDS             complement(6301..6594)
                     /locus_tag="AC16UZ_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245445.1"
                     /translation="MHRATQARNAIAPLLDQLIVQLDAEGRASQRAYFARIRRSLLLA
                     RHEADLATPIRELSTSVAVGFVFSNDADVLMSRILEKVDQLAGELEFSRPVIH"
     gene            complement(6692..>7419)
                     /locus_tag="AC16UZ_01825"
     CDS             complement(6692..>7419)
                     /locus_tag="AC16UZ_01825"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6245446.1"
                     /translation="GVSASTAPRAGPDTQGRPVEQLDRRARERLEKADALIFDFDGTL
                     APNLDLPDMRRQVVDLTLGRGVPGEIFEHLYIVEIIEAAASWLRRRDRTSAADYHEAA
                     HRLITEFELEAARNTEPFPATRTTLATLRRKGKRLGVVTRNCSTAVRTVFSDIDVYID
                     AVLARDDVDFLKPDARHVRQALSVLDGTPERAAMIGDGRLDMHVGRDLGMTCVGVLTG
                     SADADALLEAGAHIIVGALEELL"
BASE COUNT         1259 a         2358 c         2561 g         1241 t
ORIGIN      
        1 cctcgagcgg tccgaaccag caggtaagaa gcggttcgtg ctcggctgtg gccgccggcg
       61 agtcgcctgt ccctttcacc cgagcgcttc gccccgcccc tgacgcgagt ggctcaaagc
      121 ccccagcact ccatgtcgtt ggcgaatatc accgagccct ggtagtgctt gtcgatggcc
      181 tcgcggctct gggtctcacg accgcgggtc cggttcatgc ggtgcccgag aatcagcatg
      241 cgtgcgttgg cctgggcggc gatctggccg agctggctgg ggatggcgtg cagctcccgc
      301 gcggcgcctc gcgccgtctc cgggatcgcg tggctgacca ccagcgcgtc tgcgtccttg
      361 gcaaatgagg ggatgacgtt cttctggttg ctgaaatccc cggtgaagac gatggtcttg
      421 tcgcccacct ccacccgcca ggccacggcg ggaacgatgt cgtgatgaac ggggatggca
      481 gacagcctga tgtgttcggt gccgaaccgc gaccagcgac gctgcccggt ggaaggcacg
      541 tttcgcgcgt tcaccttgta gcctcccgcg gagccgtagg tcagaaaatc ggcgaggtag
      601 gggtacgcgc catccttgcc gatcatccgt tccacgaacg tcacggtgtc ggggaagacg
      661 ccctcgccgg acgggccgag caccgtcagt ggccggtcgc gctcggcaaa gtaggatcct
      721 ttgatgaagg cggggaaatc cgccgagtgg tcggcgtgca ggtgcgtgaa cgcgatggcg
      781 tccagatcct cgaattcggc cccggcgcgg tcgaaggcgg cggaagaacc gggcgccgca
      841 tccaccagca ggcgggcgtg gttgtcgcgc catatcaggt agctgggtcc ggcctggccg
      901 tcgtcgagct ccggcccgcc tgcgccgagg atctgcaccc agactccctc gtcgccgcag
      961 aacggggcat cggcggcatg agcggtgccg atagcggcca gcagtgccag gccggtgatg
     1021 ctcaagctga ggtaacgcat gctgaagtcc tcggttcggt tatcgggtcg tttcgttcaa
     1081 ggggcccctg gcgttcagcc caggtcgtcg atgtcggcaa gcaggcgctg gacgccggcc
     1141 tcgtcgggca tccgccagtc gccgcggggc gacaggctca cggagcccac ctttggaccc
     1201 gggggaaggg tggtgcgctt gaactgctgg gtgaagaagc gctggaagaa tagcctcagc
     1261 cagcgtttga tctcgtcgtc cgcgtaactc ccggtaaatg ccagcctggc cagatgaaag
     1321 atctttgccg gaccggcccc gtttctcagg aagtggaaga ggaagaaatc atgcagctcg
     1381 taggggccga tgatggcctc ggtttcctgg ctgatgctgc ccgcggtgtc gggcgcgatc
     1441 agctccggcg agatgggcgt ctgcaggacg cgctgcagga cgccggccag cgcttccccc
     1501 gcgcggtgtt tggcgtacca ccgcactagg taggccatca tggttttcgg cacgctggcg
     1561 ttgacgttgt agctcgccat gtggtccgcg ttgtacgtgc accagccgag cgccagctct
     1621 gacaggtcgc cggttcctac gacgatgccc gaaaccttgt tggcgtagtc gaagagaatc
     1681 tgggtgcgtt cccgagcctg ggtgttttcg aacaccagat cgtcacgtcc ggtgtgctcc
     1741 agcgcgtcga ggtgctgcgc caccgcctcg tgaatgggga tctccttgag cgccaccttc
     1801 gcggcggcgc agagccccct cgccgactcc agagtgtgct cggtggtccc agggcccggc
     1861 atggtgatgg cctggatgga ctggcgggga cggccgatct tgtccagggt gtccaggcag
     1921 acgaggaaag ccagggtgga gtcgagcccg cccgacaggc cgatgacgag agtttcggta
     1981 ttcgcagcga ggcatcggcg ggcgagcccc gtcgtctgga tgtcgaggat ctcggccgcc
     2041 cgggcctcga actgggcctc gtcgtcggga acgaacgggt gagccgtata gacgcgctcc
     2101 agagccgcga tgggccgggg gcgctgcagg acgtccacaa cgtggtaggg cgccgggcgc
     2161 ggcgcgttgg tgaacgtcgt gttctgcatc cggtcgtgac gcagcttggc gatgtcgatg
     2221 tccgtgatca gcgtttgggg ctcgaacgcg taacgcgggc tctcgcccag gagctggccg
     2281 ttctcgcagg cgatgaggtg cccgccgaac accaggtcct tggtcgactc cagcgggccg
     2341 ctggacgcgt agacgtagcc gcagattccc ctggcgctgg ccatgtgcac cagatccctt
     2401 cgatagtcgg ctttggccac gagctcgttg ctggcggaca ggttcacgac cagctcggct
     2461 ccggccaggc tgtgacggat gccgggcggc tccggcgccc agaggtcctc acaaatctcc
     2521 accgcgaacc gcatgtttcc gatgcgcatc agctggttgg tagccacctg gaaggacccg
     2581 agctcagggt cgtcgacgct gacgtccacc cctgcgccgg agacgaacca gcggcgctcg
     2641 tagaactcgc cgtaattcgg ctgggcgatc ttggggatca tggcgcggac cctgccgtcg
     2701 gcgatgacgg ccgcgcagtt cagcagcctg ccgtcctcca gcaggtacgg cgtgccgacg
     2761 acgcagacgg cctcgccggc ggatcgggcc agcatgagga gggccgaacg ggcggcggca
     2821 tggagctgtt cgctgaaaaa gaggtcctcg cacgagtagc cggtcagcgc aagctcgggg
     2881 aacagcgcaa tggttacagc gcgtccctcg agcgctgtca ggtgctcgac gattttctcc
     2941 gcgttggtgg ccggttcggc gatggagagt tccggcgcca cggcggcaac gcggatgaag
     3001 cccagttcgg tgtagtccat ggtgcacagt ttaactgggc tcgcgtttta aacccaaagc
     3061 atggaaaatc gcccgttctt ggcgattctg gggatgctca tgaacttcga attcaacgaa
     3121 gaccagaacc tcttgcggga gcaggcgcgg ggctttctcg ccgacaattg ctctccggcg
     3181 gtggtcagag ccgtgcttga gggtgatgcc tcctacgatg ccgacctctg gcggaagatc
     3241 gtggagatgg gttacacggc cacggtgatt cccgaggagt tcggggggct cgggctctcc
     3301 tacctggagc tgtccgtgat cgccgaggag ctcggtcgcg ttgccgctcc ggtgccgttc
     3361 tcgtcatccg tttacctggc aaccgaagcc atcctgctgg ctggctcccg ggagcagaag
     3421 gaaacctggc tgccgcgact ggccgccggc gaggtgatcg gaacgttcgc cttcggcgag
     3481 agccatgggc gccccaccgc ggagaacctg accgcccgcg tgacagacgg caagctctcc
     3541 ggaaccaagc tgccggtgcc cgacggcggg gcggcagacg ttgccgtggt cgtggcgagc
     3601 aacgggctgt acctggtgga tcttgcccag gacggggttg cggtgacgga tgttccgacc
     3661 ctggacccga cccgcggcca cagccgcatc gagttcgcgg gcgttcaagc ggagcccctg
     3721 ggcgaccccg ccgacggttg gtcgctcacc gagaaggtgc tcgatcgggc tgccgtgctg
     3781 ttcgcctggg agcaggtggg cggctcggaa gcgtcgctgg agatggccaa ggaatacgct
     3841 ctcgggcgct acgctttcgg ccgccccatc gcctcttatc aggccatcaa gcacaagctg
     3901 gccaacatgt acgtgaagaa caccctggcc aagtctaact gctactacgg ggcgtgggcg
     3961 ctcaacacgg acgcatcgga gctgaccctg gccgcggcga cggcgcgggt gtccgccact
     4021 caggcctact acttcgcctc caaggaaaac atccagaccc atggcggcat gggatacacc
     4081 tgggaattcg actgtcagtt ctactatcgc cgggcgaagc tgctgtccgt gaacatcggc
     4141 agcgaggccc actggcagga ccgtttgatc accgccgtcg agcagagcaa cgcggcataa
     4201 ggagcagtca tggatttcaa cgacaccgcg gaagaagcaa aattcagaga ggaagtgcgc
     4261 ggctggctcg aagtcaacgt gcccaccgcc gaggagctcg cagggcttaa ttacatcgag
     4321 gcggccaagc tgtggcagaa gcgcaagtac gacgccggct gggcctgcat tcgctggccg
     4381 gaggagtacg gcgggcgcgg cgcttcagcc atcgatcagg tcatctggaa ccaggaggaa
     4441 gccaagtatc ccgatctgcc gcaaggcata ttcggcatcg gccagggcat ggccgccccc
     4501 acgctgatga cctgggcgga cgacgaggcc aaggcccgct atcttccgcg ccttgccagc
     4561 ggcgaggaca tctggtgcca gctgttcagc gagcccgccg ggggctcgga ccttgcggcg
     4621 ctcaggacca aggcggagcg tgacggcgac gactgggtga tcaacggcca gaagatctgg
     4681 acctcgggcg cgcactactc agactacggg atcctcgtgg tcaggaccga ccccacggtg
     4741 ccgaagcaca aggggctgag ctatttctac ctggacatga aatccccggg catcgagatc
     4801 aagccgatca agcagatttc cggcgactcg aacttcaacg aggtctactt cacggacgtg
     4861 aggattcccg acagccagcg cctgggcgcc gtgggtcagg gctggcaggt ttcgttgacc
     4921 acgctgatga acgaacgggc ttccatcggc ggtggcggga ccgccgtcgg cttcgactcc
     4981 gttttcaagc tcgcccagcg ggtcaacatc ggcgacaggc cggccatcga ggactcccag
     5041 gtgcgggcga agcttgccga ctggttctgt caggaagccg gtctgcgctt caccggctac
     5101 cgctccatga cggcgctttc ccggggcgag atcccgggac cggaaaactc catcggcaag
     5161 ctggtcggcg cacccaagac acaggacatg gcgtctttcg ccatcgacct gatggaaatg
     5221 acgggcgcga tctgggacaa ggagttcgcc gaggaggccg ggttgttcca gggaacctac
     5281 atggccattc cgggcttgag gatcgccggc ggcaccgacg agatcatggc gaacatcatc
     5341 gccgagcggg tgctggggct gcctcaggaa ccgagaatgg acaaggggat cccgttcaac
     5401 gaggtcccca cgggatcagg ctgatctagg cgggcgccgc gcccgcgatc agctcatcga
     5461 tgcgggcggc cagcgtctgg tacacggcct cgaaggaagc cgccccggat gccggatccg
     5521 gcaggcccca gtgcagccgc tcgacgggca cgccggcccg cgaggcaaac gcggggcacg
     5581 tttcccctgc cgcgctgtcg cacagcgtga tgacccaccg cagagcgggc gccgcatcgc
     5641 ccgtgtactc gtcccaggat ttgctgcgca ggccgcgcac gtcgtgaccg tgggcccgga
     5701 gcacctccat ggcacccgaa ttcacctctc cgacggggcg gctgccggcg ctgtgggcac
     5761 gcacccgctc tccgttcacg gtcgctcgca ggtcgttggc gatggtctgg gcaatgatgc
     5821 ttcgtgccga gtttcccgtg cacaggaaca gcaccctcgc ggccggcctg ggaccccacg
     5881 cgatgggcgt cggggatccg gtggcgtagc gcggcagatc gtcgtacacg tcgaacccgg
     5941 caacgcgctc gtcgaacatg acgtgggcct cgacttcgaa tcgagccgga tcgtcgaagc
     6001 tgggcaggaa caggtgcgtc tcacccggcc agcgcgccga gcggtagcac agcggggaac
     6061 cgcagcggcc gcaatgcccg cgctcggcct ggggcgacga acggatcagg gcgggctggt
     6121 ccccgctgaa ctctacgtcc tcgctcggaa agcccgcgta ggtggcgagc agggagctgg
     6181 tgtgccgtct gcaggattcg caatgacagt gggccgtcca gaggggacga cctctggcgg
     6241 caaaccggac cgcgccgcag aggcacccgc cggtgatcgg cgcgtcgcgg gccgggctcg
     6301 tcaatggatt acgggccggg aaaactccag ctcgccggcc agctgatcga ccttctcgag
     6361 gatccgggac atcagaacgt cggcgtcgtt tgagaaaacg aagcctaccg caacagacgt
     6421 ggacagctcg cgaatgggcg tggcaaggtc ggcttcgtgt cgtgcgagaa gcagggaacg
     6481 gcggattcga gcgaagtagg cgcgttggga agcccggccc tcggcgtcca gctggacgat
     6541 gagctggtcc agcagagggg cgatggcatt gcgtgcttgc gtggctcgat gcatctcctt
     6601 ctccgggttc caggaccgac gcgactgccc acaattctga acccagtcgc cgcggaaggc
     6661 cagcctgtcg cgcctggtgc cggaagcgac gtcacagcag ctcctccaac gcgcccacga
     6721 tgatgtgggc gcctgcttca agcagggcgt ccgcgtcggc ggacccggtg agcacgccca
     6781 cgcacgtcat gcccagatcc cgaccgacgt gcatgtcgag gcggccgtcg ccgatcatgg
     6841 ccgctcgctc aggggtcccg tcgaggacgc tcagcgcctg gcgcacgtgc cgggcgtcgg
     6901 gtttgagaaa gtccacgtcg tcgcgtgcca ggacggcgtc gatgtatacg tcgatgtcgc
     6961 taaagacggt cctcaccgcc gtcgagcagt tgcgcgttac cacgccaagg cgcttccctt
     7021 tccgccgcag agtagcgagg gtggtccgcg tggccgggaa cggctcggtg ttccgggcag
     7081 cctccagctc gaactcggtg attagccgat gggcggcttc gtgatagtcg gcggccgaag
     7141 tgcgatcgcg tcgccgcagc cacgacgcgg ccgcctcgat gatctccacg atgtagagat
     7201 gctcgaagat ctccccaggc acgccgcgtc cgagggtcag gtccaccacc tgccggcgca
     7261 tgtcgggaag atccaggttg ggagccaggg tgccgtcgaa gtcgaaaatc agggcgtcgg
     7321 ccttctccag ccgctcgcgg gccctcctgt ccagctgctc caccggtctc ccctgggtgt
     7381 caggcccggc tcgcggcgcc gtgctcgccg aaacgcctc
//
LOCUS       DBNHOX010000081         4457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1291964, whole genome shotgun sequence.
ACCESSION   DBNHOX010000081 DBNHOX010000000
VERSION     DBNHOX010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1291964"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="AC16UZ_01830"
     CDS             complement(<1..887)
                     /locus_tag="AC16UZ_01830"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007352531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase domain-containing
                     protein"
                     /protein_id="HIR6245447.1"
                     /translation="MNPLVERVTERIRERSASSRSDYLRRIEEAAASGVTRARLSCSN
                     LAHGMAACPAGSKTRLSGEAAANVGIVSAYNDMLSAHQPYERYPELIRRAAEDAGAVA
                     QVAGGVPAMCDGITQGRPGMELSLFSRDVIAMSTALALSHDMFDAVVCLGVCDKIVPG
                     LLMGALAFGHLPAVFVPAGPMPSGLPNKEKARVREAYAAGEAGRVELIAAESAAYHSP
                     GTCTFYGTANSNQMLLEFMGLQLPGGSFVNPGSELRDLLTAAAVRQVLELTRLGNRHM
                     PIGRIVDESSVVNAVVGLLA"
     gene            complement(931..1572)
                     /gene="pgl"
                     /locus_tag="AC16UZ_01835"
     CDS             complement(931..1572)
                     /gene="pgl"
                     /locus_tag="AC16UZ_01835"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR6245448.1"
                     /translation="MMPGIETFPDRSAASRAAAAVLAMALSRRLATSTEAVVAVSGGT
                     TPRQCLGELAAHPLDWARVRVTLTDERCVPADHPDSNEGMVRRRLLVGRAAAARFLPL
                     QSAELRAFSAPLACALVGMGEDGHFASVFPDADNLDEALDLESNACCLRVRTQASPSA
                     RVSMTLARLLESDELVLLAFGVSKRRVLEAPGAYPVGHLLAQTRLPVRVMWAP"
     gene            complement(1569..3032)
                     /gene="zwf"
                     /locus_tag="AC16UZ_01840"
     CDS             complement(1569..3032)
                     /gene="zwf"
                     /locus_tag="AC16UZ_01840"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR6245449.1"
                     /translation="MAQLIPVDPFDLIIFGGAGDLALRKLLPALYHRHRDGQIPPGSR
                     IIAVGRSAMGRAEYLSEAARAVEAAGEGREPDIWETFAARLDYLDLDADDHSGWGPLA
                     ECLRGHEGKIRIVYLATPPHLFGSVARGFQSNSLMVPDMRIVLEKPVGDDYASARAIN
                     DEVGACFAENQIFRIDHYLGKETVQNLLALRFGNSLFEPLWRREVVDHVQITVAEELG
                     VGNRVAFYDRIGAVRDMVQNHLLQLVCLVAMEPPASLDDDAVRDEKLKVLRALKPITA
                     ANVRTSTVRAQYTAGAISGHAVPGYVDELGGATDTETFVALKLGIENWRWSGVPFYLR
                     TGKRLPAKHSEIVIQFRPVPHPLFGDVSGDVAPNRLSIMLQPDEGVRLAIMAKEPGPG
                     GFELRPVSLDLSFEETFGIRYPDAYERLLIEVMRGNPALFMRRDEIETAWRWIDDIIA
                     GWAEARQKVDAYVSGSWGPTASSLLLDRDGRHWFAGQ"
     gene            3135..3995
                     /locus_tag="AC16UZ_01845"
     CDS             3135..3995
                     /locus_tag="AC16UZ_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR6245450.1"
                     /translation="MTTGILRQIDLDLPRLSRSETRIAEWIRENPRQTVDASIGEVAA
                     NAGVSEPTVIRFCRRLGLAGFRDLKTRLVAALQSPENFLHRDVDDADGPSEAAAKVLD
                     GSIRTLVDLREQTPTMPFRAAVETMTTARELVFVGLGASGHVARDASHKFFRLGIPCS
                     TAVDAQTILQRAAIASARDVFVVVSHTGTWPEIVTAMAIAAERHARIVALTDPRSPLA
                     TTADLVFACHPVEDTNVFTPMSSRLAQLALLDALQVALALGIGEPAKQNLRASKEALS
                     EMRASACGSN"
     gene            3997..4332
                     /locus_tag="AC16UZ_01850"
     CDS             3997..4332
                     /locus_tag="AC16UZ_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014886.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HIR6245451.1"
                     /translation="MSVDRISGRRIYSRVLTGGELGAGKGINKRGGGLSALADNPRTL
                     ARVALFRGVHPELFETEHLDYELINEAAVTWLAHKGAVSRGDRVILSKGDYRNVQGGT
                     NTLKILEVA"
     gene            4356..>4457
                     /locus_tag="AC16UZ_01855"
     CDS             4356..>4457
                     /locus_tag="AC16UZ_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012274.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245452.1"
                     /translation="MGFGRVGRQIYRLALKDERFEVVAISDIGRPEIL"
BASE COUNT          801 a         1514 c         1437 g          705 t
ORIGIN      
        1 gccagcaggc ccaccaccgc gttgaccacc gaagactcgt ccacgatgcg gccgatgggc
       61 atatggcggt tgcccagacg ggtgagctcc agcacctgtc ggaccgcagc agcagtgagc
      121 agatcgcgca gttccgagcc cgggttgacg aaagatcccc ctggaagctg caggcccatg
      181 aactccaaga gcatctgatt gctgttggcc gtaccgtaga aggtgcaggt gccggggctg
      241 tgataggcgg cagactcggc cgcgatgagc tcgaccctgc ccgcctcccc ggcagcatag
      301 gcttctcgaa cgcgagcctt ctctttgttc ggcaggcccg acggcatggg gccggcgggg
      361 acgaacaccg cgggcaggtg gccgaaggcc aacgcgccca tgagcagacc gggaacgatc
      421 ttgtcgcaga ccccgagaca gaccaccgca tcgaacatgt cgtgagagag cgccagcgcc
      481 gtggacatgg cgatgacgtc acggctgaag agcgacagct ccatgcccgg acggccctgg
      541 gtgatgccgt cgcacatggc gggcaccccg ccggccacct gggcaacggc tcccgcatcc
      601 tcggcggccc ttcgaatcag ctccggatag cgctcgtacg gctggtgagc gctgagcatg
      661 tcgttgtagg ccgacacgat gccgacgttg gcggcggctt ccccggagag gcgcgtcttg
      721 gacccggcag ggcaggcggc catcccgtgg gccaggttgc tgcaggacag ccgcgcccgg
      781 gtcactccgg aggcggcggc ctcttcgatc cggcgcagat agtcgctacg gctcgacgcg
      841 ctgcgctcgc gtatccgctc cgtcacacgt tcaaccaaag gattcattca cttttcctcg
      901 agccatcgat ttccagccca cttccccact tcacggggcc cacatcaccc gaaccggcaa
      961 ccgcgtctgc gccagcaggt ggccgaccgg atacgccccg ggggcttcca gcacacgccg
     1021 cttggacacg ccgaacgcca ggagaaccag ctcatcgctt tccaggagcc gcgccagggt
     1081 catggagacc cgggcggacg ggctggcttg ggtccgaacc cgcaggcagc acgcgttgct
     1141 ttccagatcc aacgcctcgt cgaggttgtc cgcgtccggg aacaccgagg cgaaatggcc
     1201 atcctcgccc atgccgacca gggcgcacgc caacggcgcc gagaaagcac gcagctccgc
     1261 actctgcagc ggcaggaacc gcgcggccgc cgccctgcca acgagcagcc ggcgccggac
     1321 catgccttcg ttgctgtcgg ggtggtctgc cggcacgcat cgttcgtccg tcaacgtgac
     1381 gcgaacccgg gcccagtcca gcggatgcgc ggcaagctcg ccaagacact gccgcggggt
     1441 cgtcccgccg ctgaccgcca ctacggcctc ggtgctcgtt gccaaccgcc gtgacagcgc
     1501 catcgcgagc acggcggccg ccgcgcggga ggccgccgaa cggtcaggaa aggtctcgat
     1561 gccaggcatc attggccggc aaaccagtgg cggccgtccc gatccaggag gagggacgag
     1621 gccgtcgggc cccaggaacc cgaaacgtaa gcgtcgactt tctgccgcgc ctcggcccat
     1681 ccggcgatga tgtcgtcgat ccatcgccag gcggtctcga tctcgtcacg ccgcatgaaa
     1741 agggccggat taccccgcat gacctcgatg agcagccgct cgtaggcgtc cggatagcga
     1801 atgccgaagg tctcctcgaa gctgagatcg agagacacgg gccgcagctc gaacccgccg
     1861 ggcccgggct ctttggccat gatcgcgagc cgcactccct cgtcgggctg cagcatgatg
     1921 gagagccggt tgggcgccac gtccccgctc acgtcgccga acagggggtg gggcacgggc
     1981 ctgaactgga tgacgatctc ggagtgtttg gcgggcagac gcttgccggt gcgcaggtag
     2041 aacggcaccc ccgaccagcg ccagttctcg ataccaagct tgagagcaac gaaagtctcg
     2101 gtatcggtgg cgccaccaag ctcgtcgacg tacccgggca cggcgtgccc cgatatggcg
     2161 cccgccgtgt actgggcccg gaccgtggaa gtacggacgt tggcggcggt gatgggttta
     2221 agggcgcgaa ggaccttgag cttctcgtcg cgaaccgcgt cgtcgtccag gcttgccggc
     2281 ggctccatgg cgaccaggca gaccagctgc agaagatggt tctgcaccat gtcgcgcacg
     2341 gccccgatcc ggtcgtaaaa ggccacccgg tttcccacgc cgagctcctc ggccaccgtg
     2401 atctgcacgt gatccaccac ttcccgccgc cacaacggct cgaacagcga gttgccgaac
     2461 ctcagcgcca gcaggttctg cacggtttcc ttgccgagat agtggtcgat gcgaaagatc
     2521 tggttctcgg cgaagcaggc gcctacctcg tcgttgatgg ctcgcgccga ggcgtagtcg
     2581 tcgccaaccg gcttctccag aacgatgcgc atgtccggaa ccatcagaga gttggactgg
     2641 aaaccgcggg ccacgctgcc gaaaaggtgg gggggtgtgg cgagatagac gatgcgaatc
     2701 ttgccctcat gtccccgcag gcattcggcc aggggccccc agccggagtg atcgtccgca
     2761 tccaggtcga gataatccag acgtgcggcg aacgtctccc agatgtccgg ctctctccct
     2821 tcgccggcgg cttccacggc gcgcgccgcc tcggagaggt attccgccct gcccatggcg
     2881 cttcgaccca ccgcgatgat gcggctcccg ggcggaatct gcccatcccg gtggcggtgg
     2941 tacaaggccg gcaggagctt gcgcagcgca aggtctccgg caccaccgaa gatgatcagg
     3001 tcgaacggat cgacggggat gagctgcgcc actgagcgct ccactgaaca agaatttcgt
     3061 aatttgacta cattaatccc gaggatcgca attatttcgt ggccatttac caaaatacta
     3121 tgtagtttta ctacatgact acgggaatac ttcgccagat cgatctggat ctgccccgct
     3181 tgagccgctc ggaaacccgc atcgcggaat ggatccggga aaacccgcgg cagacggtcg
     3241 acgcctccat cggcgaagtg gccgccaacg caggcgtgag cgaaccaacg gtgattcgat
     3301 tctgccggcg gctgggtctt gccggcttcc gggatctcaa gacacggctg gtcgccgcgc
     3361 tgcagagccc ggagaacttc ctgcatcgcg acgtcgacga cgcggacggg ccttccgagg
     3421 ccgccgccaa ggtgctcgac gggtccattc gaaccctggt cgaccttcgc gagcagacgc
     3481 cgaccatgcc gttccgtgcc gccgtggaaa ccatgacgac ggcccgggaa ctcgtgttcg
     3541 tcgggcttgg cgcctcgggg cacgtcgcca gggatgcaag ccacaaattc tttcgcctgg
     3601 gcattccctg cagcacggcg gtggatgcgc aaacgatcct gcagcgggcc gccatcgcgt
     3661 cggcccgcga cgtcttcgtg gtcgtctccc acaccggcac ctggcccgaa atcgtcacgg
     3721 ccatggccat cgccgccgaa cgtcacgccc ggatcgtcgc tctgaccgat ccccgctccc
     3781 ccctggcgac gactgccgac ctcgtcttcg cctgccatcc ggtggaggac accaacgtgt
     3841 ttacacccat gagctcccgg ctcgcccagc tcgcgctcct ggacgcgctg caggttgcgc
     3901 tcgcgctggg catcggcgag ccggccaagc agaacctgcg ggcgagcaag gaggcgctct
     3961 ccgagatgcg cgccagcgcg tgcggatcca actgaggtga gcgtggatcg gatcagcggg
     4021 cgacggattt acagtcgcgt tctgaccggg ggcgagctcg gcgcgggcaa gggcatcaac
     4081 aaacggggcg gcggcctctc cgccctggcc gacaacccac gcacgctcgc ccgggtagcg
     4141 ctgttccgtg gcgtgcatcc cgaactcttc gagaccgagc atctggacta cgagctcatc
     4201 aacgaagccg ccgtcacctg gctcgcgcac aagggcgccg taagcagggg ggatcgagtc
     4261 attctctcca aaggcgacta ccgtaacgtg cagggcggca ccaacacact gaaaatactg
     4321 gaggtcgcct gatgcgaaag ctgcgcgtag gcatcatggg tttcggccgg gtcggtcggc
     4381 agatttaccg gctcgccctc aaggacgagc gtttcgaggt ggttgccata tccgacatcg
     4441 ggcgcccgga aatcctg
//
LOCUS       DBNHOX010000082         1877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1613260, whole genome shotgun sequence.
ACCESSION   DBNHOX010000082 DBNHOX010000000
VERSION     DBNHOX010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1613260"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..478)
                     /locus_tag="AC16UZ_01860"
     CDS             complement(5..478)
                     /locus_tag="AC16UZ_01860"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR6245453.1"
                     /translation="MQRSNPIPQAGLLALASLLASLPWADALAEGMNDEKPFAEAHVI
                     LQVSDADPSRHQTVLDIANNLTRHYGGPDMADIEIIAFSSGVKILLTTDNPHTQRIAN
                     LMENGVRFYVCGNTLDTIERRDGKRPVVLPGVREVQTGVAFMIEEIAKGYVPVHP"
     gene            complement(503..>1877)
                     /gene="soxB"
                     /locus_tag="AC16UZ_01865"
     CDS             complement(503..>1877)
                     /gene="soxB"
                     /locus_tag="AC16UZ_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HIR6245454.1"
                     /translation="LRGESGALLLDGGDTWQGSGTSLWTRGRDMVEACNLLGVDVMTG
                     HWEFTYQAQEVLDNADLLSADFVSQNVRATEDSLFEGTHVFDEDTGHVFPPYVVKELN
                     GRRIAIIGQSFPYTPIANPQRFIPDWTFGIRESDLAELVAQVRSRERVDAVVLLSHNG
                     MDVDLKLAGRVPGIDFILGGHTHDGVPVPAVVANPGGRTVVTNAGSNGKFVGVLDIKF
                     GAAGIDGYRYRLLPVFANLLEPDPQMQAHIDRVREPYLQRLEEPLAVTDSLLYRRGNF
                     NGTFDQVICDALRQVLDAQISLSPGFRWGTSVLPGQTITMENVMDQTGMTYPETYVRE
                     MRGEEIRVILESVADNLFNPDPYYQQGGDMVRVGGLRYSCAPDAPNGERIRNLELTDG
                     TPLQAGAAYKVAGWATVGERSPGPPVWDVVADYLRDQGTIRIDKANEPTLEGVKDDPG
                     IARYDCS"
BASE COUNT          312 a          586 c          617 g          362 t
ORIGIN      
        1 cccttcaagg gtgcacgggc acgtagccct tagcgatctc ttcgatcatg aaagcgacgc
       61 cggtctgtac ctctctcacg ccgggcagaa cgaccggccg cttgccgtcg cgacgctcga
      121 tggtgtccag ggtgtttcca cagacgtaga aacgcacgcc gttctccatg aggttggcga
      181 tccgctgcgt gtgcggattg tccgtcgtca gaagtatctt gacgcctgag ctgaaggcga
      241 tgatctcgat gtccgccatg tcgggaccgc cgtagtgccg ggtgaggttg ttggctatgt
      301 ccagcaccgt ctggtggcgc gaaggatccg cgtcgctgac ctgaaggatg acgtgtgcct
      361 cggcgaatgg cttttcgtcg ttcatgcctt ctgccagggc gtcagcccat ggcaggctcg
      421 ccaggaggct tgccagcgcg agcaggccgg cttgggggat agggttggat ctctgcatgg
      481 gtctcccagg acggatcgaa tctcaggagc aatcgtaccg cgcgatgccg ggatcgtctt
      541 tcacgccctc gagcgtcggc tcgttggcct tgtcgatgcg gatggtgccc tggtcgcgca
      601 ggtaatcggc gaccacgtcc cagacgggcg gccccggaga gcgctcgccg acggtggccc
      661 agccggccac tttgtaggcg gccccggcct gcagcggagt gccgtcggtg agttcgaggt
      721 tcctgatccg ttccccgttc ggcgcgtccg gcgcgcagct gtagcgcagc ccgccgaccc
      781 gcaccatgtc gccaccctgc tggtagtagg gatccggatt gaagaggttg tctgccacgc
      841 tttccaagat tacgcggatc tcttctccgc gcatttcccg gacgtaggtc tccggatagg
      901 tcatcccggt ctggtccatc acgttctcca tcgtgatggt ctggcccggc agcacggacg
      961 ttccccaccg aaaacccggg gaaaggctga tctgcgcatc gagaacctgg cgcagggcgt
     1021 cgcagatcac ctgatcgaac gtgccgttga agttgccgcg tcggtataaa agagaatcgg
     1081 tgaccgcgag gggctcctcc aggcgctgca gatacggctc gcgcacccga tcgatgtggg
     1141 cctgcatctg ggggtcgggc tcgagaaggt tcgcaaacac gggcagcagc cggtagcggt
     1201 aaccgtcgat gccggccgcg ccgaacttga tgtccagcac gccaacgaac tttccgttcg
     1261 acccggcgtt ggtcaccacc gttcttccac ccgggttggc gacgaccgcc ggtacgggga
     1321 cgccatcgtg agtgtggcca ccgagtatga agtcgattcc gggcacgcgg ccggccagct
     1381 tcaggtccac gtccatgccg ttgtgggaga gcaggaccac tgcatccacc cgctcccggg
     1441 agcgaacctg cgccacgagc tccgccagat ctgattcccg aataccgaag gtccagtccg
     1501 ggatgaatcg ctgggggttg gcgatggggg tataggggaa ggactggccg atgatggcga
     1561 tccggcggcc gtttagctcc ttgaccacgt acggcgggaa gacgtgtccg gtatcctcat
     1621 cgaatacgtg ggttccttcg aagagtgagt cctccgtggc ccggacgttc tgcgaaacga
     1681 agtcggcgct gagcagatct gcgttgtcga gcacttcctg ggcctggtag gtgaactccc
     1741 agtgtccggt catcacgtcg acgccgagaa ggttgcaggc ttccaccatg tcccgtccgc
     1801 gggtccaaag cgacgttccg gatccctgcc aggtgtcgcc gccatcgaga agcagcgccc
     1861 cggactcgcc gcgcagc
//
LOCUS       DBNHOX010000083         1887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1549101, whole genome shotgun sequence.
ACCESSION   DBNHOX010000083 DBNHOX010000000
VERSION     DBNHOX010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1549101"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="AC16UZ_01870"
     CDS             <1..575
                     /locus_tag="AC16UZ_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HIR6245455.1"
                     /translation="ARLCIGLGEGISVPSSFFLVRVPSRDQPFVFADCAVNVDPDAEE
                     LAAIALASGASAGQLLDAPPRVALLSFSTRGSATHPRVDKVRHALEIVRACRPSFPVD
                     GEFQADAALVPQVAVSKVGGDSEVAGRANVLIFPDLDAGNIAYKLTQHLAGAEVVGPI
                     LQGFARPVADLSRGAGVDEIIATTALTLCL"
     gene            593..1759
                     /locus_tag="AC16UZ_01875"
     CDS             593..1759
                     /locus_tag="AC16UZ_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HIR6245456.1"
                     /translation="MRIVEARELTVPLEGEVANAVVSFAGHTVSLVALISDQRRNGRP
                     VVGFGFNSIGRFAQGGILRERVFPRLLQAEPDTLLDPSGAAFDPAAVAAAVMRNEKPG
                     GHGDRAGAVAAVELAVWDLNAKLADEPAWATIARHFGRETQRKGAPVYAAGGYYYPED
                     SLERLRLELDSYRALGFDSFKIKIGGAPMDADMARIEAALVVAGEGSRLAVDANGRFG
                     LADALGYARALEPLRLRWFEEAGDPLDYALNADLTGAYSGPVATGENLFSGSDAVNLA
                     RFGGMRPGKDVFQMDPGLSYGLTEYAGMLAALEAQGFDRAQCWPHGGHMINLHVVVGL
                     GLGGCEAYPGVFQPFGGYAPECRIDVGRVWPSQAAGFGLEQKRELGPSLGALSA"
     gene            1815..>1887
                     /locus_tag="AC16UZ_01880"
     CDS             1815..>1887
                     /locus_tag="AC16UZ_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014601.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6245457.1"
                     /translation="MGCGAMGSVYAALLASAGHRVLAV"
BASE COUNT          254 a          612 c          685 g          336 t
ORIGIN      
        1 ccgcccgtct ctgcatcggg ctcggagagg ggatctcggt gccgtccagc tttttcctgg
       61 ttcgcgttcc gtccagggat cagcccttcg tgttcgccga ctgtgccgtc aacgtcgacc
      121 cggacgccga ggagctggcc gccatcgccc ttgcctcggg cgcgagcgcg gggcagctgc
      181 tggacgcgcc gccgcgggtg gctctgctgt cgttttcaac tcgcggcagc gcgacccatc
      241 cccgcgttga caaggtgcgc cacgcgctgg agatcgtccg cgcctgccgg ccgtcattcc
      301 ccgtggatgg tgagtttcag gcggatgccg ctttggtgcc ccaggtggcg gtgagcaagg
      361 tcggcggcga cagcgaggtg gccgggcgtg ccaacgtctt gatcttcccg gacctcgatg
      421 cgggcaacat cgcctacaag ctcacccagc atctggcagg ggcggaagtg gtgggaccca
      481 tcctgcaggg ctttgccagg cccgtcgccg atctgtcgcg gggtgcgggc gtcgacgaga
      541 tcatcgccac caccgcgctg accctttgcc tttagcgatc gtctgctgag tcgtgcgcat
      601 cgtcgaggca agagagctca cggttcccct ggagggggag gtggccaacg cggtggtcag
      661 cttcgccggg cacacggttt cgctcgtggc gctcatcagc gaccagcgac gcaacggacg
      721 gcccgtcgtc ggctttggct tcaactccat cgggcggttc gctcagggcg gcatacttcg
      781 ggagcgcgtc tttccccgcc tgctgcaagc cgaaccggac accctgctgg acccgagcgg
      841 tgcggccttc gacccggccg cggtggcagc agccgtgatg cgcaacgaaa agcccggcgg
      901 gcacggtgac cgggcgggtg cggtggctgc ggtggagctc gcggtatggg acctcaacgc
      961 caagcttgcc gacgagcccg cctgggcgac catcgcccgg catttcggcc gggaaacgca
     1021 gcgtaagggc gcgcccgtct acgctgcggg cggctactac taccccgagg actccctgga
     1081 acgccttcgc ttggaactgg attcctaccg cgccctcggg ttcgattcgt tcaagatcaa
     1141 gatcggcggc gcgcccatgg acgccgacat ggccaggatc gaggcggcgc tggtggtcgc
     1201 gggtgagggc tcgcggctcg ccgtggacgc caatgggcgt ttcgggctgg cagacgcgct
     1261 gggctatgcc agggcgttgg agcccctccg gctgcgctgg ttcgaggagg cgggcgatcc
     1321 gctggattac gcgctgaacg cggacctcac aggcgcctat tccggccccg tggctaccgg
     1381 cgagaacctg ttcagcgggt ccgatgcggt caaccttgcg cggttcggcg gcatgcgccc
     1441 cggtaaggac gtctttcaga tggatccggg cctgagctac gggctcacgg agtacgcggg
     1501 catgctggcg gcgctggaag cccagggttt cgatcgggcc cagtgctggc ctcatggcgg
     1561 gcacatgatc aatctgcacg tcgtggtggg gctcggcctc ggcggctgtg aagcctaccc
     1621 gggcgtcttt cagcctttcg gcggctacgc gcccgagtgc aggatcgacg ttggccgggt
     1681 ctggccctcc caagcggcgg gattcggttt ggagcagaag cgggagctcg gcccgtctct
     1741 cggggcgctc tcagcgtgag cggcaggtct ttcggggcag cattcaggga ggtgaaggcg
     1801 tgcacatagc catcgtgggc tgcggggcca tggggtcggt ctacgcggcc ctgctcgcca
     1861 gcgcgggtca tcgcgtgctt gccgtgg
//
LOCUS       DBNHOX010000084         2019 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1485012, whole genome shotgun sequence.
ACCESSION   DBNHOX010000084 DBNHOX010000000
VERSION     DBNHOX010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1485012"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="AC16UZ_01885"
     CDS             <1..429
                     /locus_tag="AC16UZ_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245458.1"
                     /translation="GHAEHQGSFNVVYNRTVWGDVDMTLNYGLVFSTDVFNIPGGDED
                     TLVDEDGVPGDRGGEAIPGYDVHHLSASFSRDSWTVQAFVDNLWNEYYITGTRTSRRF
                     LQDENLGPGNTINGFTLRSYGQYVGMPRNIGISVSYTFER"
     gene            497..>2019
                     /locus_tag="AC16UZ_01890"
     CDS             497..>2019
                     /locus_tag="AC16UZ_01890"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     sulfotransferase family protein"
                     /protein_id="HIR6245459.1"
                     /translation="MQDSVAELRKEAARALNQRAYERAAVLCRRLLQQDPKCADGWFL
                     MGMVAAANGRITKALELVGNAALLDADNAEYLAQKARLHLLLSQPEAARETADRALAA
                     RPEDPLTLDTLGVVYTRTGNYDQARQLLQRAVAAADDNPQFHFNIASVEQFLGNLEAA
                     AAGYERSIELHPRFFRARWALSELRKSDSDARGIDVLEHLLADSQLTAEDELYLAHAL
                     ATEYEKDARHDDAFRVLARAKARRRARIGYAHENDARLFTAIRDAFPVGSLPARAQGD
                     AGSDAIFVIGMPRTGTTLVERILDSHPEVQSLGEIQDFAMAVKTASGSRSGLILDEDV
                     ISRARDADALQIGQSYLQSIAARLGHDGRFVDKMPLNFLYAGFILSTLPGARIVCLKR
                     HPLDTCISSFRQLFALDFSYYNYHYDLQDTARYYALFRQLMGHWQACGHGRIHEVSYE
                     ALTADPESEVRHLLSYLELPWHPACLEFHRNRGAVATASTVQVRQPLYRSSVSRWKR"
BASE COUNT          364 a          669 c          643 g          343 t
ORIGIN      
        1 ggccacgccg agcatcaggg atccttcaac gtcgtctaca accggacggt gtggggcgac
       61 gtggacatga cgctgaacta cgggctcgtc ttctccacag acgtcttcaa tattcccggc
      121 ggcgacgagg acacgctggt ggacgaggac ggcgtgccgg gagaccgggg cggcgaggct
      181 atcccgggtt acgacgtgca tcacctgtcg gcatcgttca gccgcgattc ctggaccgtt
      241 caggcctttg tcgacaatct gtggaacgag tactacatca cgggcacccg cacctcgcgc
      301 cggttcctgc aggacgagaa tctcggaccc ggcaacacga tcaacggctt cacgctgcgc
      361 tcgtacggcc agtacgtggg catgccgcgc aacatcggga tcagcgtgag ctacaccttc
      421 gagcgttgag cccgccctta ccggcctggc cagcagcagc gttgtatcgt tgccgcggcc
      481 ggcctaaggt gcgcggatgc aagattcagt tgccgagctc aggaaagaag ctgccagagc
      541 gctgaaccag cgggcctacg agcgagccgc cgtgctgtgc cggcgtcttc tgcagcagga
      601 cccaaagtgc gccgacggat ggttcctgat gggcatggtg gcggccgcca acgggcggat
      661 caccaaggcc ctggagctcg tcggcaacgc cgcgctgctg gacgccgaca atgcggagta
      721 cctggcccag aaggcgaggc tgcacctcct gctgagtcag ccggaggcgg cccgggagac
      781 ggcagaccga gcgctcgccg ccaggccgga ggacccgctc accctggaca cgctgggcgt
      841 ggtctacacg cgcacgggta actacgacca agcccggcag ttgctgcagc gcgcggtggc
      901 cgccgcagac gacaatcccc agttccactt caatattgcg tccgtggagc agtttctcgg
      961 caacctggaa gccgcggccg ccgggtacga gcgcagcatt gagctccatc cgcgcttctt
     1021 ccgggcgcgc tgggccctgt cggagctgag gaagtcggat tcggatgccc gtggcatcga
     1081 cgtgctcgag cacctgctcg ccgattcgca gctcacggcc gaggacgagc tctatctggc
     1141 ccacgccctt gcgacggagt acgaaaagga cgcgcgccac gacgacgcgt tccgggtgct
     1201 ggctcgggcc aaagccaggc ggcgcgcccg catcggatac gcccatgaga atgatgcgcg
     1261 gctgttcact gcgataagag acgccttccc cgtcggttcg ctgcccgcac gggcgcaggg
     1321 agatgcaggc agcgatgcca tcttcgtgat cgggatgccg cgaacgggca cgacgctcgt
     1381 cgagcgcatt ctggacagtc atcccgaggt tcaatcgctt ggcgagattc aggacttcgc
     1441 catggcggta aagaccgcgt cgggctcacg cagcggcctg atcctggacg aagatgtcat
     1501 atcgcgagcc cgcgacgcgg acgctttgca gatcggtcaa agttatctgc agtccatcgc
     1561 ggcccgactg ggacacgacg gcaggttcgt cgacaagatg cccctgaatt ttctctatgc
     1621 cggcttcatt ctcagcaccc tccccggcgc ccggatcgtc tgtctgaaac ggcatccgct
     1681 ggacacctgc atcagcagct tccggcagct tttcgccctg gatttctcct actacaacta
     1741 ccactacgat ctgcaggaca cggcccgcta ctacgcgctg ttccgacagc tgatggggca
     1801 ctggcaggcc tgcggccacg gacgcatcca cgaggtctcc tacgaggccc tgactgccga
     1861 tcccgaatcc gaggtgcggc atttgctcag ctacctcgag cttccctggc acccggcatg
     1921 cctggagttt catcgcaatc gcggcgccgt ggccaccgcc agcacggtgc aggtgaggca
     1981 gcccctgtac cggtcctcgg tgtcgcgatg gaaacgcta
//
LOCUS       DBNHOX010000085         4995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_778126, whole genome shotgun sequence.
ACCESSION   DBNHOX010000085 DBNHOX010000000
VERSION     DBNHOX010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_778126"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1734)
                     /locus_tag="AC16UZ_01895"
     CDS             complement(<1..1734)
                     /locus_tag="AC16UZ_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6245460.1"
                     /translation="MTLELPGFQVQAEIGHGATSRVYLAMHKQLSRLVALKILAEEYG
                     NDPAFRVRFLNESRIGSSLSHPNIVQVYDTGAHDNTLYLVLEYVRGGNLLQRVDRGMH
                     IRDLVRVVQNIGKALDYAHARGLVHRDVKPENILFREDGSAVLTDFGIAGFVDPDGPP
                     AKGGTVPGTPQYMSPEQAAGKALDGRSDLYSLGIVFFRVLTGDVPYKADTAVSLGIKH
                     LQEPIPRLPNHLAIFQDVVNRSLAKKPDQRFQSGQEFADALEALVASGQVPSLMIKAR
                     AVAPEEIRSLGSNVFARARDSGRGERQSAPERRPLTLGRAALAASLVAAVLAFGIVVV
                     EQPQWFTRMLAVTGIAEDPEVEDAWSNAQSLHQDPNQSLATIVAGYRRVVNLDPDHEG
                     ARAAIAGLASQWKSSIEDSLAAENLEQAGTKLEESLTVFPEDPSLVELTEQLTNRQHA
                     ESLLASTQALLRSHGLSDIPSATAAIQAYQEVLRLAPDHAVATEELNSLAQHYASLAA
                     EAADTGEVDAAISFLDRASAANSELRELDLVRSKIQQATTLQAAIIELLQQASAYRAA
                     GALINPPGENAA"
     gene            complement(1734..2726)
                     /locus_tag="AC16UZ_01900"
     CDS             complement(1734..2726)
                     /locus_tag="AC16UZ_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014543.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HIR6245461.1"
                     /translation="MQVAHHSTAARRLAFTVLFPALVLGSQALARTETIELANGDLYE
                     GEVVDDLRTGQGIYVWADGHRYEGQFEANRMHGQGVYFWPDGRTYTGSFVTDRREGRG
                     VLRWPNGDVYEGEFRDNKMHGTGRFVWANQDAYVGDFRDGSRSGQGQFEWSTGERYTG
                     GFDDGVRVGRGIFEWPDGRRYEGGFAGDAKSGKGVLEWPNGNRFVGDFAEDARSGLGI
                     FYWRDGTVYQGQYAQDKMHGYGIKEQPDGAMELQRWQEGALQFGKPLLADARCSLTID
                     SQPWMFYSTQCINGLAHGQGLAVSLDGRLIVEEGRFVLGNLIEGEIRVLRLGES"
     gene            2767..3570
                     /locus_tag="AC16UZ_01905"
     CDS             2767..3570
                     /locus_tag="AC16UZ_01905"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR6245462.1"
                     /translation="MNHLFFFGSCVAVGALSGFLGGLLGIGGGIVIVPALIILFDTME
                     LFPQSIATPVAVGTSLAVIIFTSLAAARTQIRAGMVDWDVVRRWAPFLIIGSFLAGPI
                     AVALPVATFRTLIGAFLLFVSCVMLTNWKPDPHRKFPGAVAIAGIGMLGGLISGIAGV
                     GGGNVMVPTLVYFNKPVHRATATSSTLGVPIATAGTLGFVYTGWNAELGPALLGFVYL
                     PAFVAIVLATVVTAPMGVRVAYRVAPLPLRRGFGLLLVVIAARTLYSAF"
     gene            complement(3597..>4995)
                     /gene="pyk"
                     /locus_tag="AC16UZ_01910"
     CDS             complement(3597..>4995)
                     /gene="pyk"
                     /locus_tag="AC16UZ_01910"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR6245463.1"
                     /translation="RRRTKIICTIGPATANYPTLERLQAAGMNIVRLNMSHADHASAK
                     KIINWIRTLNRKVQYPVAILLDTQGPEIRTGEINEPMDLSTGDVVTISVLPEDVERSS
                     IHVNYRELVDILQVGNRMTVDNGLLNFEVLEKKGQQLVCRVIDGGVLRSKSHVNLPGV
                     RVNLPSITPKDRRDVEFGIENDVDFIALSFVRSAEDVTELRGILGAKAKNIKIIAKIE
                     DQEGVTHVHDIVRAADGVMVARGDLGIETNIADLPNVQRRIVHSAAKWGKRSIVATHL
                     LESMIENPIPTRAEVTDVANAIYEGVDAIMLSGETSVGKHPVRCVEQLDDIARSSERW
                     PGLGYEKELVANTDKQHIANNAVALAEDIHAKGIVVITRRGITADLVTSARPRNVPIY
                     AFTNHSQTRRRMSLNRAVFGHRMQFSSDPETTLQRALKILRDREGFEPGDKVVVISDV
                     LAEKNSDAIQLRHLD"
BASE COUNT          794 a         1641 c         1581 g          979 t
ORIGIN      
        1 ggccgcgttc tcgcccgggg ggttgatgag cgcgccggct gcccgatagg cgctggcctg
       61 ctgcagcagc tcgatgatgg ctgcctggag cgtcgtcgcc tgctgaatct tcgatcgaac
      121 caggtccagc tcacgcagct ccgagttggc ggctgacgcc cgatcgagga agctgatcgc
      181 cgcatccacc tcgcccgtat ccgccgcttc cgcggccagc gaagcatagt gctgtgcaag
      241 ggagttgagc tcctccgtgg caacggcgtg gtcgggcgcg agccgcagaa cctcctgata
      301 ggcctggatg gcagccgttg cagagggaat gtcggagagc ccgtgactgc gcagcagagc
      361 ctgggtgctg gcgagcaggc tttccgcgtg ctgccggttc gtcagctgct cggtgagctc
      421 gacgagcgag ggatcctcgg gaaaaaccgt cagcgactct tcgagcttcg tacccgcctg
      481 ctcgagattc tccgccgcaa gagaatcctc gatgctgctc ttccactggc tggccagccc
      541 ggcaatggcg gcgcgagcgc cctcgtggtc gggatccaga ttcaccaccc gccggtaacc
      601 ggcaacgatg gtcgccaggc tctgattggg atcctggtgc agagactggg cgttgctcca
      661 ggcatcttcc acctcgggat cctcggcgat gcccgtgacg gcaagcatgc gggtgaacca
      721 ctgaggctgc tcgacgacga cgatgccaaa ggcgaggacc gccgctacca gcgacgccgc
      781 cagagccgct ctgcctaggg tgagggggcg ccgctccggc gcggattgac gctcgccccg
      841 cccggagtcc cgggcccggg caaagacgtt cgatcccagg gagcggatct cttccggcgc
      901 cacggcacgg gccttgatca tgaggctggg cacctgtccc gacgccacca gggcttcgag
      961 ggcgtcggca aactcctgac cgctctggaa gcgctggtcg ggctttttcg ccaggctgcg
     1021 gttgacgacg tcctggaaga tggccaggtg gttggggagc cgcgggatcg gttcctgcag
     1081 atgcttgatt ccaagactca ccgcggtatc ggccttgtag ggaacgtccc cggtcagaac
     1141 acggaaaaaa acgattccca ggctgtacag atccgaccgg ccatccagcg ccttgccggc
     1201 ggcctgctcg gggctcatgt actgcggcgt ccccggtacg gtgccgccct tggccggcgg
     1261 accgtccgga tcgacgaacc cggcgatgcc gaagtcggtg agcacggcgc tgccgtcctc
     1321 gcggaaaaga atgttctccg gcttcacgtc ccggtggacg agccctcggg catgggcgta
     1381 atccagcgct ttgccgatgt tctgcaccac gcgaaccaga tcacggatgt gcatgccccg
     1441 gtccacgcgc tgcagaagat tcccgccccg aacgtattcg aggaccaggt agagagtgtt
     1501 gtcgtgggcg cccgtatcgt aaacctggac gatgttcgga tgggacaggc tggatccgat
     1561 gcgcgactcg ttcagaaagc gaacgcggaa ggcgggatcg ttaccgtatt cctccgcgag
     1621 gatcttgagc gccaccaggc ggctcaactg cttgtgcatg gcaagataga ctcgggaggt
     1681 ggccccgtga ccgatctccg cctgcacctg aaatccggga agctccaacg tcactacgac
     1741 tctcccagtc tgaggacccg gatctccccc tcgatcaggt tgccgagcac gaacctgccc
     1801 tcctcgacga tcagccgccc gtccaggctg accgccagac cctggccgtg agcgagcccg
     1861 ttgatgcact gcgtgctgta gaacatccat ggttgcgagt cgatggtgag cgagcaacgc
     1921 gcatcggcca gcagaggctt gccgaactga agcgcgccct cctgccagcg ctgcagttcc
     1981 atggcgccgt caggctgctc cttgatgccg tagccgtgca tcttgtcctg ggcgtactgg
     2041 ccctggtaga cggtgccgtc acgccagtag aagatcccca gtccggaacg ggcatcttcc
     2101 gcgaaatctc cgacgaaccg gtttccgttc ggccactcca atacgccctt gccggacttc
     2161 gcatcaccag caaagccgcc ctcgtatcga cgcccgtcgg gccactcgaa gatcccacgg
     2221 cctacccgga cgccatcgtc gaagccgccc gtgtatcgct cgcccgtgct ccactcgaac
     2281 tggccttgcc ccgagcggga cccgtcgcgg aagtcgccga cgtaggcgtc ctggttggcc
     2341 cacacgaacc ggcccgtgcc gtgcatcttg ttgtcgcgga actcgccctc gtagacgtcg
     2401 ccgttaggcc agcgcagcac gcctcggccc tcccgacggt ctgttacgaa ggatcccgtg
     2461 taggtgcgtc catccggcca gaagtagacg ccctgaccat gcatgcggtt ggcttcgaac
     2521 tgcccttcgt agcggtgccc gtcggcccag acgtagatcc cctgacccgt gcgcagatcg
     2581 tcgaccacct ctccttcgta aagatcaccg ttggcgagct cgatggtttc ggtacgggca
     2641 agcgcctggc tgcccagaac cagcgccggg aaaagcacgg tgaacgcgag ccgtcgtgcg
     2701 gcggtggaat ggtgagctac ctgcaagttc agtagactcc cgagatcgtc gagtacgtta
     2761 ctttaattga atcatctttt cttctttggt tcctgcgtcg cagttggcgc cttgagcggc
     2821 tttctcggcg gtctcctggg catcggcggc ggcatcgtca tcgtccccgc cctcatcatc
     2881 ctgttcgata ccatggaact ctttcctcag agcattgcca cgcctgtggc cgtgggaacc
     2941 tccctcgccg tgatcatctt cacgtccctc gcagcggcgc gaacccagat acgggcgggc
     3001 atggtggact gggacgtggt ccggcgatgg gcgccctttc tgatcatcgg cagcttcctt
     3061 gcgggaccca tcgcggtcgc ccttccggtg gccaccttcc gcaccctgat cggggcattt
     3121 ctcctcttcg tatcctgcgt gatgctcacg aactggaagc cggatccgca ccggaaattt
     3181 cccggggccg tggcgatcgc gggcatcggc atgctgggcg gcctcatctc cgggattgcc
     3241 ggtgtcggcg gggggaacgt gatggtgccc acgctcgtct acttcaacaa acccgtgcac
     3301 cgggccaccg cgacgtcgag caccctcggc gtgcccatcg ccaccgccgg caccctgggt
     3361 ttcgtctaca ccggctggaa tgccgagctt ggccccgccc tgctcggctt cgtgtatctg
     3421 ccggcgttcg tggccatcgt gctcgccacc gtcgtgacgg cgccaatggg cgtgcgcgtc
     3481 gcctatcggg tcgcgccact gccattgcgc agaggattcg gcctgcttct ggtggtcatc
     3541 gcggcgcgga cgctctactc ggcgttctga cacctgccag cggcggccgt cgggattcag
     3601 tccaggtgcc gcagctgaat ggcgtcggag tttttctcgg ccagcacgtc cgagatgacg
     3661 acgaccttgt cgccaggctc gaacccctca cgatcccgca ggattttcag cgccctctgc
     3721 agcgtggtct cgggatcgct ggagaactgc atgcgatggc cgaagacggc ccgattcagc
     3781 gacatgcggc gccgcgtctg gctgtggttg gtgaacgcgt agatgggcac gtttcgcggc
     3841 cgcgcgctcg tgacgagatc ggcggtgatg ccgcgccggg taatgaccac gatgcctttg
     3901 gcatgaatgt cctcggccag cgctaccgcg ttgttggcga tgtgctgctt gtcggtgttg
     3961 gccacgagtt ccttttcgta tccgagcccg ggccaacgct ctgatgaacg ggcgatgtcg
     4021 tcgagctgct ccacgcagcg caccgggtgc ttgcccacgc tggtctcgcc ggagagcatg
     4081 atggcatcga cgccctcgta gatggcgttg gcgacgtcgg tcacctcggc ccgggtggga
     4141 atggggtttt cgatcatcga ctccagcagg tgcgtcgcca cgatggagcg tttgccccac
     4201 tttgccgccg agtgcacgat gcgccgctgc acgttcggca gatcggcgat gttcgtctcg
     4261 atgccgagat cgcccctggc caccatcacc ccatcggccg cgcgcacgat gtcgtgcaca
     4321 tgggtcaccc cttcctgatc ttcgatcttc gcgatgatct ttatgttctt cgccttcgcg
     4381 cccaggatgc cgcgcagctc ggtgacgtcc tccgccgagc gcacgaagga cagcgcgatg
     4441 aagtccacgt cgttctcgat gccgaactcc acgtcccggc gatccttggg cgtgatcgat
     4501 ggcaggttca cacgcacccc cggcaggttg acgtgagatt tgctccgcag aacgcccccg
     4561 tctatcaccc ggcagacgag ctgctgacct ttcttctcca gcacctcgaa gttgagcaga
     4621 ccgttgtcga cggtcatccg gttgcccacc tgcaaaatgt ccacgagttc ccgatagttg
     4681 acgtgaatgg agctgcgctc gacgtcctcg ggcagcacgc tgatggtcac gacgtcgccg
     4741 gtgctcagat ccatgggttc gttgatctcg ccggtgcgga tctcggggcc ctgggtatcg
     4801 agcaggatgg cgaccggata ctgcaccttg cggttgaggg tgcggatcca gttgatgatc
     4861 ttctttgccg aggcgtggtc cgcatgggac atgttcagac ggacgatgtt cattcccgcc
     4921 gcctgtagcc tttccagcgt ggggtagttg gccgtggctg gaccgatggt gcagatgatc
     4981 ttggtacgtc gtcgt
//
LOCUS       DBNHOX010000086        10552 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_650015, whole genome shotgun sequence.
ACCESSION   DBNHOX010000086 DBNHOX010000000
VERSION     DBNHOX010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10552)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10552)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10552
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_650015"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3939)
                     /locus_tag="AC16UZ_01915"
     CDS             complement(<1..3939)
                     /locus_tag="AC16UZ_01915"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6245464.1"
                     /translation="MTPLAQFEPGVRAWFTERFPSPTAVQAQSWPRIAAGEHVLITAP
                     TGSGKTLTAFLWALNQYASGSWVPGHTRVVYISPLKALNNDIRRNLLEPLRSLREDHE
                     FPPVSVQTRSGDTPQSERQRMLRRPPDILITTPESLSLMLTTTRGRLALGSVETVILD
                     EIHSIVDNRRGAQLMTSLERLTDLCGEIQRLALSATVRPLEGIAEYVGGYTADGAPRR
                     VSIVDPGSAKEIEFRVRYPDEVRLALENGRKIWDPLSEVFRRQIEHNASTLFFTNSRR
                     LAEKITLKINEDQAGPIAYAHHGSLAREIRTEVEERLKAGSLKAIVATSSLEMGIDIG
                     HLDEVVLVQSPPSIAATLQRIGRAGHGVGETSVGTLYPTHANDFLEAIVLAKAVAGGD
                     IEPLTPQENALDVLAQIIVSMCASESWQLDDVFALLRRSTPYHALPREQFDLLIDMLA
                     GRYAGSRVRELKPRIVLDRINNTIRAHKGAVLALYNSGGTIPDRGYYQLRHADTGAVI
                     GELDEEFVWEATTGQIFSMGTQNWQVTRVTHNDVLVRSAPPGTTAPPFWRSESYNRSF
                     HFSERIARELEQAEALLDDGRNDDLRSTFTSAGGFEPEAAVELLDYLTRQREASEAPL
                     PHRHHLLLELVRSGPGGYRGPDDTRQLVLHTFWGGRLNRPWALALEAAWQDALGRRAE
                     IHADNNAIVIQVKGEPDPARVLAMVTADNLDELLRRSLERSGFFGARFRECAGRALLL
                     PRQRFNQRLPLWMSRLQAKKLMSATAGYPDFPMLLESWRTCLKDEFELDSLRQMLTEL
                     EDGTIEWTFVSTSSPSPFAANVTFAQINRYMYADDTPEEDAGPSALSDDLIRSAVRNA
                     GLRPPIRREIIQEYLAKRQRTAEGYRPVSAENWSEWVKERVLIPAAEVRAATSADAPV
                     PEHEHLVWLEERAGSGENRAWLTHRELLHGLAQRGLRDGLAFQGTVPDVEESRSAEQL
                     ALEILSFYGPLTESDIAWILPRVPESLLDENEVLVSGALVADDPGLYYCDAENYAALL
                     RFQRAAARPELQPRPFHTLPGFLASWQRFGVSGGDEHVLEVMEPLRGFRAPVRAWLED
                     VVSARIPAMADSQLDDVLLRHGMQWRGAGRKAIAIGYPEDLQLIDDGVDEPSTLAKLF
                     TDPTARYPFVQLADRSGLSGEAFNEQWWASVWEGEITSDTLAPLRQGLERGYALNAAA
                     PQRASSHRQGRRRARPAPAWTGNWSVKPPFDADIDPLTELEDAKDRARMLLDRYGIIC
                     RELANREGRLLRWRCVFRALRLMELAGEVTAGYFFTGLSGPQFVSPGALRR"
     gene            complement(3936..4784)
                     /locus_tag="AC16UZ_01920"
     CDS             complement(3936..4784)
                     /locus_tag="AC16UZ_01920"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF044908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6245465.1"
                     /translation="MIHGNPADLVDLLSVHPTREEGYQAGQVREARSRVYGGQFLGQG
                     ICAASQIGHGALRSFHGYYLRPGATDLGIGYQVERLGDMVVGVSAVQREKQLFLMMAR
                     FGPFCPAPPEEMPDVPPPEDCIPRERGIAGLDRDTDSTWAVTDSPFDNRFVEDIWNED
                     YRRPLHHVWFRLRDPGALPGHIDQIVMQAIAAYYADDTIMDNALFPHGWHRCWSELQT
                     ASLDHAMWFHRPMDLRRWHLFAQDSPVASGGRAMTRGTIFDDRGAVVASAAQEILLRE
                     PPDDKP"
     gene            4854..6071
                     /locus_tag="AC16UZ_01925"
     CDS             4854..6071
                     /locus_tag="AC16UZ_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024732.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HIR6245466.1"
                     /translation="MKELSALLEPGQRIFVAGSSNEPTGLLEVLADQTLPPEIEFLQF
                     PLAGYNRFDFTAFSEDAIFTTFFMTPHLRDADPARVRFLPMQMRAVYDFLAEGVDVVL
                     LQVARNASGQLCVGPNVDFVEAAMMGAKTVAAELNHGFVAPAGCPVVDAGRIDFLMET
                     DRSLFEMAPPEIDGAAARIGEHVAQLVRDGDCIQTGIGSIPAAILARLGDRNDLGMHG
                     GLIDDGGMALIRNGNMTGVNKPIDTGKHIAGMALGSRQLLDWLAQTPAVQFRGANHTH
                     EVNVIRELPQFLSVNSAVEVDLFGQVNAEVAGGRQISGTGGSVDFMRAAKASPGGRSI
                     VAMNATARGGKVSRIVPKVELVTALRTDVDIIVTEHGVAQLRALSAAERVRALIEIAA
                     PEFRDGLRKVASG"
     gene            6232..6474
                     /locus_tag="AC16UZ_01930"
     CDS             6232..6474
                     /locus_tag="AC16UZ_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245467.1"
                     /translation="MDYVGIDPAVGLECGHCKHISRLSYSGLLDLVLECERAECDGCG
                     RLMMHDWTTVTVVQNIIKKRMRQANEAKVRHLVHHA"
     gene            complement(6465..8564)
                     /locus_tag="AC16UZ_01935"
     CDS             complement(6465..8564)
                     /locus_tag="AC16UZ_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014896.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR6245468.1"
                     /translation="MIRPPVAKRRNHRHTHHGITLEDAYYWLRDPAYPDVKDPEIIEY
                     LTAENAYFDARMEAHQPLVTQLFEEIKGRQPAEDASVPHRHGAFWYQWRYQKDAQYRQ
                     WLRAPAPAGSSPDAGPWEVILDEPELAREHPYFSLGGISVSRSGRFIAWSADTSGAER
                     FTLRIRDLQTGELVDDPIEQTQGSPVWAADDTCFFYVVVNDQWRPYQVRRHKLGDPPD
                     ADAVVYEEPEDSFFVSVDETQSEAYILIAAGDHVTSEIRYVPADEPLAEPALIARRRA
                     GHEYEVDHREGAFYVVTNDAHKNFRLARAPEDDPVEQNWQTVIEGSESHYLTGLLCFK
                     DHLVVCERIDGLDQIRIRSGEEEHHVVFPESAYAVGLGTNAEYDSATLRLHYESMVTP
                     STVYDYDIARRKLIQRKVREIPTGYDRTRYETARRTVTARDGAQVPLSIVYPRDFPRD
                     GTAPVHLYSYGAYGHAMSPTFSPSRLSLLDRGFAFAVAHVRGGDDLGRQWYEDGKLDK
                     RTNTFNDFVDCARHLIDSGFSSPGLVVISGGSAGGELMGAAINAAPTLWGAVVAHVPF
                     VDVLSTMLDAGLPLTPIEWPEWGNPIEDKAAFELIRSYSPYDQLAPGAYPPILVTAGL
                     NDPRVTYWEAAKYVAKLRHLKTDENWLLLKTNMSAGHSGESGRFDSLRELAEEYAFFL
                     VSLEAAGWHPRGEDDQA"
     gene            8663..9310
                     /locus_tag="AC16UZ_01940"
     CDS             8663..9310
                     /locus_tag="AC16UZ_01940"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6245469.1"
                     /translation="MAHVQPRSIDDVPALKPIFDAAESAMGFVPNSMLTMAHMPQLPM
                     GFMMLTNVIFGGDLKAMADQLQPLIPEQGDAGENLSPPLIQLIAFATSISSGCRYCQA
                     HTAHSGHRQGAEQEKFDSVLDYENSPVFSDAERAAIGLALAAGAVPNQADAMHFERLR
                     AYFSERQIVQIVAVIAIFGFLNRWNDTMATQLEAPAVDYAAASLGELAWAIGKHG"
     gene            9322..10020
                     /locus_tag="AC16UZ_01945"
     CDS             9322..10020
                     /locus_tag="AC16UZ_01945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HIR6245470.1"
                     /translation="MCGRFNVTSSPLSELLLELVGLPHPGPDNYNAAPTETIVVLRMN
                     DDGRPELVAMRWWLTPFWSKSVTTKYSMFNAKSETVEKSPAFREPFSRRRCVVPVSGF
                     YEWARSKERKLPYYLTPHLASGMLLAGIWDSWQDGENGPLLSFAVLTCEANEQLTFVH
                     KRQPVMLGMKDARIWLDPSTQASALKALCAPRVPVALDAVPVSSYVNNARNKDSRCTE
                     AIGTAVAIREDVTA"
     gene            10017..10478
                     /locus_tag="AC16UZ_01950"
     CDS             10017..10478
                     /locus_tag="AC16UZ_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245471.1"
                     /translation="MILIRSGQWTGRGSILAEGQSLGQGVACDVEVQRDDEGASISAE
                     VDAKELGSWAMNVRIAPDEQGTYDLSVRTLKGSLTGTAKLDSPPNLGLLWNEEQTVHA
                     TFTLFEIAGGLGFRGFLRDGKTTYTWEIALSLRQDHIGGENVVSLVSRRRR"
BASE COUNT         1794 a         3299 c         3502 g         1957 t
ORIGIN      
        1 acgccgcagc gcgccgggtg acacgaactg gggtccagac aggccggtga aaaagtagcc
       61 ggccgtcacc tcgcctgcca gttccatgag gcgcagtgcc ctgaagacac atcgccaccg
      121 cagcagacgc ccttcgcgat tggcgagctc ccggcagatg atgccgtatc ggtcgagcag
      181 catgcgggcg cggtccttgg cgtcctccag ctcggtgagc ggatcgatgt cagcgtcgaa
      241 cgggggcttc accgaccagt ttcccgtcca ggcaggcgcc ggcctggcgc gccgtcgtcc
      301 ctgccgatgg ctggaggccc gctgcggcgc cgcggcgttc aaggcgtagc cgcgttcgag
      361 tccctgccgt agcggggcga gggtgtccga ggtgatctcg ccttcccaga ccgacgccca
      421 ccattgctcg ttgaatgctt ctccagaaag tccgcttctg tcggccagct gcacaaacgg
      481 gtatcgcgcg gtcggatccg tgaagagctt ggccagggtt gatggctcat cgacgccgtc
      541 gtcgatgagc tgcagatcct cgggatagcc gatggctatc gccttgcgcc cggctccgcg
      601 ccactgcatg ccgtgacgca gcagcacgtc gtcgagctgg gaatcggcca ttgccggaat
      661 gcgggcggaa acgacgtctt cgagccacgc ccgcacgggg gcgcggaacc cgcgcaaggg
      721 ctccatgacc tcgagaacgt gctcatcccc gccgctcacg ccgaagcgct gccagcttgc
      781 caggaaaccg ggcagggtat ggaagggccg gggctgcagt tccggcctgg ctgccgcgcg
      841 ctgaaaccgc agcagggccg cgtagttttc cgcatcgcag tagtacagcc ccgggtcgtc
      901 tgccaccagg gcgccgctca cgaggacctc gttctcatcg agcaggctct cgggaacccg
      961 gggcagaatc caggcgatgt cggactccgt caaggggccg tagaaagaca ggatctccag
     1021 ggcgagctgc tcggcacttc ggctctcctc gacgtccggg accgtccctt ggaaggcaag
     1081 gccgtcccgc agaccccgtt gggcgagccc gtggagcaac tcccgatggg tgagccaggc
     1141 gcggttctct ccggagccgg cgcgctcctc cagccagacg aggtgctcgt gctcgggtac
     1201 cggcgcgtcc gcactggtgg cggcccggac ctcggcggcc ggaatcagaa cccgctcctt
     1261 gacccattcg gaccagttct cggcgctcac cgggcggtat ccttcggccg tgcgctgccg
     1321 tttcgccagg tactcctgaa tgatttcgcg ccggatgggc gggcgcaggc cggcgttgcg
     1381 cacggcgctg cggatcagat cgtcggacag cgcgctgggc ccggcgtctt cttccggggt
     1441 gtcgtcggcg tacatgtagc ggttgatctg ggcgaaggtg acgttggccg cgaaggggct
     1501 gggcgagctg gtggacacga acgtccactc gatggtgccg tcctccagtt cggtcagcat
     1561 ctgtcgaagc gagtcgagtt cgaactcgtc cttcaggcag gtgcgccagc tttcgagaag
     1621 catggggaag tcggggtagc cggccgtcgc ggacatcagc ttctttgcct gcagccggct
     1681 catccacagc ggcagccgct ggttgaagcg ttgcctcggc aagagcaggg cccggccggc
     1741 gcactctcga aaacgcgcgc cgaagaatcc ggaccgctcc agggacctgc gcagcagctc
     1801 gtcgaggttg tcggccgtca ccatggcgag cacccgcgcg ggatcgggtt cgccctttac
     1861 ctgaatgacg atggcgttgt tgtcggcgtg aatttccgcg cgcctgccga gcgcgtcctg
     1921 ccaggcggcc tccaatgcca gcgcccacgg ccggttcagc cggccgcccc agaacgtatg
     1981 cagcaccagc tggcgggtat cgtccggtcc gcgataaccg cccggtccgc tgcggacgag
     2041 ttccagcagc agatgatgcc gatggggcag gggggcttcg ctcgcctccc gctgccgggt
     2101 caggtaatcg agcagctcga cggccgcctc gggctcgaag ccgcccgcgc tggtgaacgt
     2161 ggatcgaaga tcgtcgttgc gcccgtcgtc gagcaaggct tcggcttgct cgagctcgcg
     2221 ggcgatgcgc tccgagaagt ggaagctccg gttgtacgac tccgagcgcc agaacggcgg
     2281 agccgtcgtg cccggtggtg ccgagcgaac gagcacatcg ttgtgcgtca cccgcgtgac
     2341 ctgccagttc tgggtgccca tgctgaagat ctgacccgtg gtggcctccc agacgaactc
     2401 ctcgtcgagc tcgccgatca ccgcaccggt gtcggcgtgg cgaagctggt aatagccgcg
     2461 gtcggggatg gtgccgccgg agttgtacag ggcgaggacc gcgcccttat gcgctcgaat
     2521 cgtgttgttt atccggtcca gaacgatgcg cggtttcagc tcccgcaccc gtgagcctgc
     2581 atagcgcccg gccagcatgt cgatcagcag atcgaactgc tcccgcggca gcgcatggta
     2641 cggggtgctg cggcgaagca gagcgaagac atcgtcgagc tgccagcttt cgctggcgca
     2701 catggacacg atgatctggg cgagcacgtc cagcgcgttc tcctgaggag tcagcggctc
     2761 gatgtcgccc ccggctaccg ccttggccag gacgatggcc tcgagaaagt cgtttgcgtg
     2821 ggtagggtag agcgtgccga cgctggtctc tcccacgccg tgaccggccc gaccgatgcg
     2881 ctgaagcgtg gccgcgatgg aaggcggaga ctggaccagg acgacctcgt cgagatggcc
     2941 gatgtcgata cccatctcca gagagctggt cgcgacgatg gccttgaggc tgccggcctt
     3001 cagccgctct tccacctccg tgcggatctc tctggccagc gagccgtggt gagcgtaggc
     3061 gatgggcccg gcctggtcct cgtttatctt caaggtgatc ttttcggcca ggcgccggct
     3121 gttggtgaaa aacagagtcg aggcgttgtg ctcgatctgg cgccgaaaaa cctctgacaa
     3181 cggatcccag atcttcctgc cgttctccag ggcgagcctg acttcgtcgg ggtagcgcac
     3241 gcggaactcg atttccttgg cggagcctgg atcgacgatg ctgaccctgc gcggagcgcc
     3301 gtcggccgtg tagccgccca cgtactccgc gatcccttcg agtgggcgga cggtagcaga
     3361 cagcgcgagc cgctgaatct cgccgcacag atccgtcagt ctttccagcg aggtcatcag
     3421 ctgcgcgccg cgccggttgt ccacgatgga atgtatctcg tcgagtatca ccgtctccac
     3481 gctgccaagc gccagtcgtc cccgagtggt ggtcagcatc agggacaggc tttcaggcgt
     3541 cgtgatcaga atgtccggcg gccgccgcag cattcgttgt ctttcgcttt gcggcgtgtc
     3601 gccggatcgg gtctgcacgc ttaccggggg aaattcgtga tcctcccgaa gcgatcgcag
     3661 gggctccagc aggttgcgcc ggatgtcgtt gttcagcgct ttcagcggcg agatgtagac
     3721 aacccgggta tggcccggca cccacgatcc ggacgcgtac tggttcaacg cccacagaaa
     3781 agcggtcagc gttttgccgc tgcccgtcgg tgccgtgatc agcacatgct cccctgctgc
     3841 gatccgcggc cagctctgcg cctgaacggc agtgggggaa ggaaagcgct cggtgaacca
     3901 cgcacgtact ccgggttcga actgcgccag aggtgtcacg gcttgtcgtc cggtggttcg
     3961 cgcagaagga tctcctgggc ggctgaggcc accaccgcgc cccgatcgtc gaatatggtc
     4021 ccacgggtca tggcgcgccc gccgcttgcc accgggctgt cctgggcaaa gaggtgccag
     4081 cgtctgagat ccatgggtcg gtggaaccac atggcgtggt cgaggctcgc cgtctggagt
     4141 tcgctccagc atcggtgcca gccgtgtgga aagagcgcgt tgtccatgat ggtgtcgtcc
     4201 gcgtagtagg cggcaatcgc ctgcatcacg atctgatcga tatggccggg cagcgcgccg
     4261 ggatcgcgca ggcggaacca gacgtggtga agcggtcgcc ggtagtcttc gttccagatg
     4321 tcctcgacga accggttgtc gaaaggggag tctgttacgg cccatgtgga atcggtgtct
     4381 cggtccaggc cggcaatgcc gcgctcccgt ggaatgcagt cctcgggggg cggcacgtcg
     4441 ggcatctcct cgggtggagc ggggcagaaa gggccgaagc gcgccatcat gagaaacagc
     4501 tgcttctcgc gctgcacggc ggacacgccg acaaccatgt cgccgaggcg ctccacctgg
     4561 tagccgatgc ccaggtccgt cgccccggga cgcagatagt agccgtgaaa ggacctcagg
     4621 gcgccgtgcc cgatctggct ggcggcacag atgccctgac cgagaaactg gcctccgtat
     4681 acccgcgagc gtgcttcgcg cacctgaccc gcttgatagc cttcctctct cgtcgggtgg
     4741 actgaaagca aatcgaccag gtctgcgggg ttgccgtgaa tcatctgccg attattacga
     4801 attgcccctg ggcgaggcta gaatctcgcc cttgcctcca ggatggtgaa atcatgaaag
     4861 agttatcggc tctgctggag ccgggtcaga ggatattcgt ggcgggcagt tccaacgagc
     4921 cgaccgggtt gctcgaggtg ctggcggatc agactctgcc gccggaaatc gagttcctcc
     4981 agtttccgct ggctggatac aaccgcttcg acttcacggc cttctccgag gacgcgatct
     5041 tcacaacctt tttcatgacg ccgcatcttc gcgatgccga tccggcgcgc gtgcgtttcc
     5101 ttccgatgca gatgcgagcc gtgtacgact tcctggccga aggtgtcgac gtcgtgctcc
     5161 tccaggttgc gcgcaatgcc agcggccagc tctgcgtagg ccccaacgtg gacttcgtgg
     5221 aggccgcgat gatgggcgcg aagaccgtgg cggccgagct gaatcacggc ttcgtcgccc
     5281 ccgcgggttg tccggtggtc gacgccggtc ggatcgactt tctcatggag acggaccgtt
     5341 cgctgtttga gatggctcct ccggagatag atggggcggc ggcgcgcatc ggtgagcacg
     5401 tcgcgcaact cgttcgcgac ggcgactgca ttcagaccgg cataggcagc atccctgccg
     5461 ctatccttgc aaggctcggc gacaggaatg atctcggcat gcacggcggg cttatcgacg
     5521 acggcgggat ggcgctcatc cgtaatggga acatgaccgg cgttaacaag cccatagaca
     5581 caggaaaaca catagccggc atggcgctcg gatctcgcca gctgctcgac tggctggctc
     5641 aaaccccggc ggtacagttc cggggagcca accacactca cgaggtcaac gtgatacgcg
     5701 agctgccgca gtttctttct gtcaactccg ccgtcgaggt ggacctgttc ggccaggtca
     5761 atgccgaggt cgctggcggc aggcagattt ccggaacggg cggctccgtc gacttcatgc
     5821 gcgcggcgaa agcctctccg gggggacgat ccatcgttgc catgaacgca accgctcggg
     5881 ggggcaaggt ttcccgcatc gtgccgaaag tcgagttggt taccgcgttg cgcacggacg
     5941 tggacataat tgtcacggag catggcgtcg cccagctccg tgcgctgtcg gcggccgagc
     6001 gcgtacgcgc gctcattgag atcgcggcgc cggaattccg tgatggcctg cggaaagtgg
     6061 cctccggctg atacccgtcg ttcggcagtg actgcaaggc ttggtctggc gcgtcaggcg
     6121 ccactgcgtc atagcaggag tgtgcacgga gtcacaaagc cgctaaagta ccgctcaatc
     6181 acgaaatccg tcctttagaa tggcgggcgt gacgaaaggg agtacagcgt catggattac
     6241 gtaggcatcg atcccgcggt gggcctggag tgcggccact gcaagcatat ctcacgtttg
     6301 tcctacagcg gtcttctgga ccttgttcta gaatgcgagc gcgccgagtg cgacgggtgc
     6361 gggcgtctga tgatgcacga ctggacgacg gtcaccgttg tgcagaacat catcaagaag
     6421 cgcatgcgcc aggccaacga ggcaaaggtc cgtcacctgg ttcatcacgc ctgatcgtcc
     6481 tcgccgcgcg gatgccatcc ggcggcttcg aggctcacca gaaagaaagc atattcctcg
     6541 gccagttcgc gaagcgagtc gaaccggccg gactctccgc tatggccggc agacatgttg
     6601 gttttcagca ggagccagtt ctcgtctgtc ttgagatgcc gcagcttggc gacgtacttt
     6661 gcggcctccc agtaggtgac tctggggtcg ttgagtcctg cggtaaccag gatgggcggg
     6721 tacgcgccgg gcgcgagctg gtcgtagggt gagtatgatc tgatcagctc gaaggccgct
     6781 ttgtcctcga tcgggttacc ccactccggc cactcgatgg gggtgagcgg caggccggca
     6841 tccagcatcg tgctcagcac gtcgacgaag ggcacgtgag cgaccacggc tccccacagc
     6901 gttggcgctg cgttgatggc agcacccatc agctcgcctc cggcgctgcc gccggatatg
     6961 acgaccagcc ccggtgaaga gaatccggag tcgatcaggt gccgagcgca atccacgaag
     7021 tcgttgaacg tgttggttcg cttgtcaagc ttgccgtctt cataccactg tcgtcccagg
     7081 tcgtcgcccc cgcgaacgtg cgcgacggcg aaagcgaagc cccgatccag aagggacaga
     7141 cggctcggcg aaaatgtcgg gctcatggca tggccgtacg ctccgtagga gtagaggtgc
     7201 acgggcgcgg ttccgtcgcg cggaaagtct ctggggtaca cgatgctcaa gggtacctgc
     7261 gcgccgtcgc gagcggttac ggtgcgccgc gcggtttcgt atcgtgtccg gtcgtagccg
     7321 gttgggattt cccggacttt gcgctgaatc agcttgcgcc gtgcaatgtc gtaatcgtag
     7381 accgtgctgg gcgtgaccat ggattcgtag tgcagccgca gagttgcgct gtcgtattcc
     7441 gcattcgttc ccaggccgac cgcgtatgcg ctctccggaa acacgacgtg gtgttcctct
     7501 tcgccgctgc ggatacgtat ctggtccagg ccatcgatcc gctcgcacac caccaggtga
     7561 tctttgaagc agagcaggcc ggtgaggtag tggctctcgg agccttcgat gaccgtctgc
     7621 cagttctgct cgacaggatc gtcttccggg gctcgggcga ggcggaagtt cttgtgcgcg
     7681 tcgttggtga cgacgtagaa ggcgccttcg cgatgatcca cttcgtactc gtggcccgcc
     7741 cttctccgcg cgatcagagc cggctctgca agaggctcgt cagccggaac gtacctgatt
     7801 tccgaggtca cgtgatctcc ggcggcaatg aggatatagg cttcggactg ggtctcgtcc
     7861 acggaaacga agaacgagtc ttcgggctcc tcgtacacga cggcgtcagc atcgggcggg
     7921 tcgccgagct tgtggcggcg cacctgatac gggcgccatt gatcgttgac gaccacgtag
     7981 aaaaagcacg tgtcgtcggc cgcccatacc gggcttccct gggtttgctc tatgggatcg
     8041 tcgacgagct cgcctgtttg cagatccctg atcctgagag tgaagcgttc ggctccgctc
     8101 gtgtcggcgc tccacgcgat gaagcggccg ctgcggctca cggagattcc gcccaggctg
     8161 aagtacgggt gttcccgggc gagctccggc tcgtcgagaa tcacttccca tggtccggca
     8221 tcaggagagg aacccgcggg tgccggtgcg cgcagccact gtcggtactg ggcatccttc
     8281 tgatagcgcc actgatacca gaaggcgccg tgtcgatggg gcacggaggc gtcctccgcg
     8341 ggctgacgac ccttgatctc ctcgaacagt tgcgtcacca ggggctggtg ggcctccatc
     8401 cgagcatcga agtacgcgtt ctctgccgtc agatattcga tgatctcggg atccttgacg
     8461 tcggggtagg cgggatccct cagccagtaa tacgcatcct ccaaggtgat gccgtgatgg
     8521 gtgtggcggt ggttgcggcg tttggcgacg ggtggtcgga tcattcgagc ctcgagcgtt
     8581 cgttggctcg gcagtgtggc gaatgtaagc caaaacgcaa accagtaaac tcaagcaacc
     8641 caactcattg gagcgcaagc ccatggcgca cgtgcagccc agatcgatag acgacgtccc
     8701 ggcgctgaag ccgatattcg atgctgccga gagcgccatg gggttcgtgc ccaacagcat
     8761 gctgaccatg gcccacatgc cgcagctgcc gatgggtttc atgatgttga cgaacgtgat
     8821 cttcgggggt gatctcaagg ccatggccga tcagctccag ccgctgatcc ccgagcaggg
     8881 ggatgccggc gagaacctgt cgccgcctct gattcagctc attgccttcg caaccagcat
     8941 cagctcgggc tgccgttatt gtcaggccca caccgcccac agcggacacc gtcagggcgc
     9001 cgagcaggaa aagttcgatt ccgttctcga ctacgagaac agccccgtct tctcggacgc
     9061 cgagcgcgcg gcgatcggtc tggcgcttgc tgccggtgcc gtgcccaatc aggccgatgc
     9121 tatgcacttc gagaggctgc gggcgtattt cagcgagcgg cagatcgtgc agatcgtggc
     9181 ggtgattgcc atcttcgggt tcctcaaccg ctggaacgac accatggcga cccagctcga
     9241 ggcgcccgcg gtggactacg cggcggcgtc actgggcgag ctcgcctggg ccatcggcaa
     9301 gcacggctga gggcgcgcgc cgtgtgcgga cgtttcaacg ttacctccag cccgctttcg
     9361 gagctgctgc tcgagctggt cgggctgccg catccgggac cagacaatta caatgccgcg
     9421 ccgacggaga ccatcgtcgt tctgcgtatg aacgacgacg ggcggccgga gctcgttgcg
     9481 atgcgctggt ggctcacccc cttctggtcc aagtcggtga cgacgaagta cagcatgttc
     9541 aacgccaagt cggagaccgt ggagaaaagc ccggctttcc gggaaccctt cagccggcgt
     9601 cgatgcgtgg tgccggtcag cgggttctac gagtgggctc gcagcaagga gcgcaagctg
     9661 ccgtattacc tgacgcctca tctggcttcc ggcatgctgc tggccggaat atgggacagc
     9721 tggcaggacg gtgaaaacgg accgctgttg agcttcgcgg tgctcacctg cgaggcgaac
     9781 gagcagctga cgttcgttca taaacgccag cccgttatgc tcggtatgaa agacgcccgg
     9841 atctggctcg atccttcgac gcaggcctcc gccctcaaag cgctctgtgc tccgcgcgtt
     9901 cccgtcgcgc tggacgcggt gccggtgtcc agctacgtca acaatgcccg caacaaagat
     9961 tcccgctgca ccgaagccat cggcacggcc gtggccatca gggaggacgt caccgcatga
    10021 tactcattcg atcgggccag tggacgggcc ggggctcaat cctcgccgag gggcagagtc
    10081 tggggcaggg ggttgcctgt gatgtggagg tgcagcggga cgacgagggt gcaagcatca
    10141 gcgccgaagt ggatgccaag gagctgggct cgtgggccat gaacgtcagg atcgccccgg
    10201 acgaacaggg gacgtatgac ctgagcgtgc gcaccctcaa gggttctctg accggaacgg
    10261 ccaagctgga cagccccccg aacctggggc tcctctggaa cgaggagcag accgttcacg
    10321 cgacgtttac tcttttcgag attgccggcg ggctcggctt ccgggggttt ctccgcgacg
    10381 gcaagaccac ttacacctgg gagatcgccc tctcgctgcg gcaggaccat atcggcggag
    10441 agaacgtggt ttcgcttgtc agccgccgtc ggcgctgaga tccagccagg ccctctccgc
    10501 gtcgctcagc gccacttcaa gcgctgcgag gctgctgcgg gtctcccacg gc
//
LOCUS       DBNHOX010000087         2069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_842515, whole genome shotgun sequence.
ACCESSION   DBNHOX010000087 DBNHOX010000000
VERSION     DBNHOX010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_842515"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="AC16UZ_01955"
     CDS             complement(<1..378)
                     /locus_tag="AC16UZ_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245472.1"
                     /translation="MSEIALPPRTAYPWYLTSSSLWMAGMSLQGFLFTWLLVGILERP
                     ADEAGLARSLAEFPPLAVLLLGGLLGDRFDNRAYLGVMHLLMAVPPLLIAAVYLNGAL
                     SSWWVVLFGVLMASIQALSDPARQ"
     gene            493..1350
                     /locus_tag="AC16UZ_01960"
     CDS             493..1350
                     /locus_tag="AC16UZ_01960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6245473.1"
                     /translation="MNIGAVMAFNHVMSPQFMADAAQMVENAGFHSVWLPEHVLFFPD
                     YESRYPYTEDGRIPGDPEGVLDPFTALTWIAAATKRIRLGTGICLVPQRQPVYTAKMV
                     ADLDYLSGGRVDFGIGIGWLKEEFDNLQMDFRARARRCLEYVEVMQALWSPGLSSYEG
                     ETVTLKPCHFNPKPVQQPHPPIFFGGESDPALARVVSHGQGWYGFNLSPEGLAGRKAR
                     LAEIAAEAGRDMNDVRIFIGPSGKPVTPETVSEYEDLGVEQLIVPVIAGNLERLAQRI
                     DKARAMTGL"
     gene            1488..1754
                     /locus_tag="AC16UZ_01965"
     CDS             1488..1754
                     /locus_tag="AC16UZ_01965"
                     /inference="COORDINATES: protein motif:HMM:NF039725.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6316 family protein"
                     /protein_id="HIR6245474.1"
                     /translation="MNVGRRNEKPKTWFRSERVFMNDGSWFFRTREGVEIGPYETQSE
                     ATMEAGMLRELLRDRGHDGKGIAVIREFVRDSYAMGRPLTPKFD"
     gene            1819..>2069
                     /locus_tag="AC16UZ_01970"
     CDS             1819..>2069
                     /locus_tag="AC16UZ_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245475.1"
                     /translation="MPRLKQISRADAPEHIQRMYGFLFGDRDPVAEPGTDTGTPGNWW
                     TVFALVPDCFDHAVAGFQLYRSPKRELEPTLRELGQTRAG"
BASE COUNT          387 a          651 c          684 g          347 t
ORIGIN      
        1 ctgcctggcc ggatcgctca gcgcctggat gctggccatg agcacgccga acagaaccac
       61 ccaccaagag ctgagcgcgc cgttgaggta aacggcggcg atcagcagcg ggggaacggc
      121 catgagcagg tgcatgactc ccagataggc ccgattgtcg aaccggtcgc ccagcaggcc
      181 gccgagcagc aggacggcca gcggtgggaa ctcggccagc gatcgggcga ggcccgcctc
      241 atccgccggc cgctccagga tgcccactaa aagccaggtg aaaaggaagc cctgaaggct
      301 catgcccgcc atccacaggc tggagctggt caggtaccag gggtaagcgg tgcgtggggg
      361 cagcgcgatc tcggacaagc tgggtgcctt ggtggtgtcc tttgccgggc gggcatcata
      421 accttagcgg tttccggcgg cttcagccgg gttgaggcgg gccgtttctg cactgactgg
      481 gaggtggacg agatgaatat cggggcggtg atggcgttca accacgtgat gagtccgcaa
      541 ttcatggcgg acgcggcgca gatggtggag aacgccggtt tccactcggt ctggctgccg
      601 gagcacgtgc tgttctttcc ggactacgaa tcgcgttatc cctacaccga agacggccgt
      661 atcccgggtg atccggaagg cgtcctcgat cctttcacgg ccctgacgtg gatcgccgcg
      721 gcgaccaagc gcatacgtct gggcaccggg atctgcctgg tgccccagcg tcagcccgtc
      781 tacaccgcca agatggtggc ggatctcgac tacctctccg gcgggcgggt ggacttcggc
      841 atcggtatcg ggtggctcaa ggaggagttc gacaacctgc agatggactt cagggcccgg
      901 gctcggcgct gcctcgaata cgtggaagtc atgcaggctc tctggagccc gggtctgtcc
      961 agctacgaag gcgagacggt gactctcaag ccgtgtcact tcaaccccaa gccggtccag
     1021 cagcctcatc cgccgatctt tttcggcggc gagagcgatc cggcgctggc gcgcgtggtc
     1081 tcgcatgggc aggggtggta cggattcaat ctgagtccag aggggctggc cgggcgcaaa
     1141 gcgcggctgg cggagattgc cgccgaggca ggtcgggata tgaatgacgt ccgcatattc
     1201 atcggcccca gcgggaagcc cgttacgccc gagaccgtat cggagtacga ggacctgggc
     1261 gtcgagcagc tcatcgtgcc ggtgatagcc ggcaacctgg agcgcctggc gcagcgtatc
     1321 gacaaggccc gcgccatgac ggggctctga gccgccggca aacgcaggca tgacacgatc
     1381 atgcggaata tggcgggtcc gggaactatc cgtaacaact ttcctacaga ctggcagggg
     1441 gtcacgccgc aaactggttc gacctgatta gccgtctttt tgccgcgatg aatgtagggc
     1501 gacgaaacga aaagcccaaa acctggttcc gttccgagcg cgtcttcatg aacgacggca
     1561 gctggttctt ccgcacccgc gaaggcgtgg agatcggccc ctacgagacc cagtccgaag
     1621 ccaccatgga agccggcatg ctccgagagc tgctccgcga ccggggacac gacggcaagg
     1681 gcatcgccgt gatccgcgaa ttcgtccgcg actcctacgc catgggtcgc cccctcacgc
     1741 ccaagttcga ctaaccccct gacccgccgc ccacaaatcg gcgcataatg tccccccgac
     1801 ccctgggagg agttagacat gccgcgtctc aagcagatca gccgagccga tgcgccggaa
     1861 cacatacagc ggatgtacgg ttttcttttt ggcgaccgcg atccggtggc cgagccgggc
     1921 actgatacgg gcactccggg aaactggtgg acggtgtttg ccctggtgcc cgattgcttc
     1981 gaccacgccg tcgccggctt tcagctttac cgcagcccca agcgggagct ggagcctacc
     2041 cttcgcgagc tcggccagac ccgggccgg
//
LOCUS       DBNHOX010000088         6526 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_842664, whole genome shotgun sequence.
ACCESSION   DBNHOX010000088 DBNHOX010000000
VERSION     DBNHOX010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_842664"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..1607
                     /locus_tag="AC16UZ_01975"
     CDS             264..1607
                     /locus_tag="AC16UZ_01975"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR6245476.1"
                     /translation="MMWNDSESEIFAQMAGRLAAAFADLPAFSAGLLDRDAVEVLRET
                     AQRLADNYPYFHPLYAGQMLKPPHPVARLAYGLAMLINPNNHALDGGRASSEMERAAV
                     AELAAMFGWQTHLGHLTSGGTMANLEALWIAGALQPGGVVLASEQAHYTHERLSGVLR
                     LPFEPIAADASGKMRMDALEDRLRRGGVGTVVATVGTTGFGAVDPLADIVELRHRYGF
                     RLHADAAYGGYFVLAGNLQAETRRHFDAMGAVDSIVVDPHKHGLQPYGCGSVLFRDPA
                     VGAFYKHDSPYTYFTSEALHLGEISLECSRAGAAAVALWATHRLLPPRPGGEFARGLE
                     RCRDAAEALHGRLAGHHRFLPLVQPELDIVAWAVLDADAEEASAAGRRLFEEAGELGL
                     HLALVQAPGSMIAHRWPEGADIPEGPVTCLRSVLMKPEHLDWLDRIWSLLEDLAG"
     gene            1902..2684
                     /gene="panB"
                     /locus_tag="AC16UZ_01980"
     CDS             1902..2684
                     /gene="panB"
                     /locus_tag="AC16UZ_01980"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR6245477.1"
                     /translation="MLDFPARKLNREKISMVTCYDFWSAQILEQTEVDCLLVGDSLAM
                     VMHGFDSTVHADTALMALHVAAVRRGAPSKFIVADMPFLAARKGLQPAMDAVQTLMQA
                     GANAVKIEGEAGQAELMTHIVQSGVPVMGHLGLTPQSVHTIGGHKVQGRTDEAAEQLR
                     SSAEALAAAGCFALVLECVPSRLAAKVTRAIDIPTIGIGAGPETDGQVLVLQDLLGMN
                     PDFKPKFLRHYANGFESVSGAVNRFHADTQRGCFPSKSESYA"
     gene            2681..3457
                     /gene="panC"
                     /locus_tag="AC16UZ_01985"
     CDS             2681..3457
                     /gene="panC"
                     /locus_tag="AC16UZ_01985"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR6245478.1"
                     /translation="MKVLSSLGDWRAFRAGLVGEIGFVPTMGALHEGHAQLLQRSREA
                     NDHTVVSIYVNPTQFNNPADLDSYPGTLGEDLCRARELGVNAVLIPSYDELYPDDFRF
                     RVEEHEFSRELCGAHRPGHFTGVMTVVLKLLNLVRPRRAYFGEKDYQQYLLIRDMCDA
                     FFVDVEIVPCATVREEDGLALSSRNALLDEKGRARAGRLNRLLRDAPSDAAASRALEQ
                     AGFEVDYVVTKADRRFAAATLRCGEREVRLIDNVAIAQHG"
     gene            3472..4242
                     /locus_tag="AC16UZ_01990"
     CDS             3472..4242
                     /locus_tag="AC16UZ_01990"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR6245479.1"
                     /translation="MLLCLDVGNSQIYCGVYDGDRLTVQFRRTSTVRSSSDELGVFLR
                     GALRENGVSPDGITQIAICSVVPDVLYSLRSCCQKYFGLEPLIMRPGIKTGLKISYKD
                     PKEVGADRIADAIGAIKLYPNRNLIVVDFGTATTVCAISRDREFLGGNIVPGVRLAMD
                     ALEEKTAQLPSVEIFPVTSAIGRSTVESIQNGLYWSNVGMIKELVARVTEEAFPDDAP
                     VVIATGGFATLFDGLGLFDAIVSDLILIGLREAVRLNA"
     gene            4311..5060
                     /locus_tag="AC16UZ_01995"
     CDS             4311..5060
                     /locus_tag="AC16UZ_01995"
                     /inference="COORDINATES: protein motif:HMM:NF021511.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HIR6245480.1"
                     /translation="MPQPRFADARHRQFALGLLGVYLLIWTSLAIKPLYRADWLLENV
                     LVAIALPMLVATYHRLPLSRISYAMLFIFFCIHAVGSHYTYAEVPYDDWTRRLFGTSL
                     GEAAGWDRNHYDRFVHLMYGLLIVYPVRELFLRVADARGFWGYFFPLLVVMSSSLAYE
                     LLEWGAAFVFGGELGMAYLGTQGDVWDAHKDSLLATGGALAATLVIAAVHSSFDRDFG
                     RDWSQSLTVKNPDPLGEAAIGRYLNERENAP"
     gene            5057..6247
                     /locus_tag="AC16UZ_02000"
     CDS             5057..6247
                     /locus_tag="AC16UZ_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR6245481.1"
                     /translation="MSPLLPSAGGGLKLFSIFGIEIRLDYSVAIIFGLVVYSLGGGLF
                     PQWHPDWPPSTAWLTALVAGVLFFASLLAHELSHSLTARRYGIRVPRITLFLFGGVAE
                     IESEAQTPSAELTIAIAGPLMSALLGAVFTVVAFGSMDTLAREAFAEDPETVIASLSP
                     GYTACLWLGSVNMMLAVFNLIPGFPLDGGRVFRAIIWKLTGNQLRATRLASTTGRWFG
                     WAIMGIGLWNLLVLKSAGGLWLLLIGWFLSHLAAASYSQALAQQTLAPLRVRDVMRTR
                     FEFAPSGITIDEFINDYLLRSNQILWPLQGPAGVVGAVNFEQVASVPPDQRATRLVQE
                     VMVPVDRLPALDADDLATNALNTLLGAGDQPVPVVSGQRIVGLVRGSDILKWVMLHQG
                     GTPS"
     gene            complement(6272..>6526)
                     /locus_tag="AC16UZ_02005"
     CDS             complement(6272..>6526)
                     /locus_tag="AC16UZ_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HIR6245482.1"
                     /translation="RGLHEKNLARTLEDGGTITSPLVPWNTCGAYMAATLGVPTLAYL
                     PFCFFNLANPFVSAAYGYAGFKIEPLKPGGEGEAAPSRAQ"
BASE COUNT         1075 a         1979 c         2159 g         1313 t
ORIGIN      
        1 ggtgaggttg tagttcatcg ggtttggatc cgtcgggtcg cgggcagcgg gggattctaa
       61 tgcattgcac agccctcaga taccgatgcg acgcgcaacc gttcagatgc cccggggttg
      121 cgcgcgatat ccctagcagg gaggatcgtc cgaggtcgat gagaggcagc tgtcggggga
      181 tcgagcgctc agggccctca tccttaggtg gtctcgccca tgctgatccg gcggcatgag
      241 cagggcaatg gagagctagg ctgatgatgt ggaacgactc cgagtcggag atattcgcgc
      301 agatggctgg tcgactcgct gcagcatttg ccgaccttcc agccttcagc gccgggctcc
      361 tcgaccgtga tgcggtcgag gttctgcggg agaccgcgca gcgtctcgca gacaactacc
      421 cgtacttcca cccgctgtac gcggggcaga tgctgaagcc gccgcatccg gtggcccggc
      481 tcgcatacgg tcttgccatg ctcatcaatc cgaacaacca cgcgctggat ggaggccgcg
      541 ccagctccga gatggagcgg gcggccgtgg cggaactggc agccatgttc ggttggcaga
      601 cccatctcgg acatctcacc agcggtggaa cgatggccaa cctggaagcg ctgtggatcg
      661 ccggcgcgct gcagcccggt ggggtcgtgc tcgcctcgga gcaggcgcac tatacccatg
      721 agcgcctgag cggcgtgctg aggctgccgt tcgagccgat cgccgcagat gcctcgggga
      781 agatgcggat ggatgcgctg gaggatcggc tccgccgcgg tggcgtgggt acggtggtcg
      841 ctaccgttgg caccacgggc ttcggagccg tagatcctct cgccgacatc gttgaattac
      901 ggcaccgcta tgggtttcgg ctgcacgccg atgccgctta cggtggctat ttcgttctcg
      961 ccggcaacct tcaggcagag acccgtcggc atttcgatgc catgggtgca gtcgattcca
     1021 tcgtggtgga tccgcacaag catggactgc agccttacgg ctgcggcagc gtgctgtttc
     1081 gcgatccggc ggtgggcgcg ttttacaagc atgactcgcc ttacacgtac ttcacctccg
     1141 aggcgctgca tctcggggag ataagcctgg aatgctcgcg ggcgggggcg gcggccgtcg
     1201 cgctgtgggc aacccatcgg ttgctgcccc cgaggccggg tggcgagttc gcccgggggc
     1261 tcgagcgctg tcgcgacgcc gctgaagctc tccacggccg actcgccggt caccacaggt
     1321 tcctgcccct cgttcagccc gagctcgaca tcgtcgcatg ggcggtgttg gatgcagatg
     1381 ccgaggaggc cagcgcggcg ggccggcgcc tgtttgagga agccggtgag ctcgggctgc
     1441 acctggcgct ggtccaggcg ccggggtcga tgattgctca ccgctggccc gagggcgcgg
     1501 acattcccga gggaccggtg acctgtctgc ggtcggtgct catgaagccg gaacatctgg
     1561 attggctgga ccgaatctgg tcactgctgg aggatctggc cggctgaaac tccattcacg
     1621 cggaaggaga aaggggtttt gggtctcatt ttgcgcccgt tgcgcgttca ccggagcgga
     1681 tggactttcc tgtgagagtg acgtagggtt ttctgcggct ccgatgctca gcacggagac
     1741 cgtggcgccc cgcattgacg tcggcgccag gcagagattt gaaccgccat aggcaattgg
     1801 acttgatccc gatgaaagga caggtacctg aagcagaacg gcgtgcgccg ctcgtgcggg
     1861 tactgcatcg gtgatcgcaa ccggagggcc ccgcagtgaa tgtgcttgat tttcccgcgc
     1921 gcaaacttaa tcgagaaaaa atctcgatgg tcacctgtta tgacttctgg tctgctcaga
     1981 tcctggagca gaccgaggtg gattgtctgc tggtcggtga cagcctggcg atggtcatgc
     2041 acggattcga ctcgacggtg catgcggata ccgcgctgat ggcgttgcac gtcgccgccg
     2101 tgcgtcgggg cgcgccgtcg aaattcatcg tcgccgacat gccgtttctt gccgcccgca
     2161 aggggctgca gcccgcgatg gacgccgtcc agacgctgat gcaggccggc gcaaacgccg
     2221 tgaagatcga gggcgaagcg gggcaggccg agctgatgac gcacatcgtg cagtcgggcg
     2281 ttccggtcat gggacacctc gggctcaccc ctcagtcggt acacaccatc ggcgggcaca
     2341 aggtgcaggg ccggaccgac gaggccgccg agcagctgcg gtcctctgcc gaggcgctgg
     2401 ccgcggccgg ttgctttgcg ctggttctcg agtgcgtgcc ctcgcggctc gccgcaaagg
     2461 tcacccgggc catcgacatt ccgacgatcg gtattggagc cggaccggaa acggatgggc
     2521 aggtgctcgt tctgcaggat ctgctcggca tgaatcccga tttcaagccc aagtttctgc
     2581 gtcattacgc caacgggttc gagtcggtga gcggcgccgt caaccggttt cacgccgaca
     2641 cccagcgcgg ctgctttccg tcgaaatcgg agtcctacgc gtgaaagtcc tctccagcct
     2701 cggggattgg cgggcgttcc gcgccgggct cgtcggcgag atcgggttcg tgccgaccat
     2761 gggcgcgctc catgaaggtc atgcccagct gctccagcgc agtcgggaag cgaacgacca
     2821 cacggttgtg agcatctatg tgaaccccac tcagttcaac aatcccgccg atctcgacag
     2881 ctaccctggg acgctcggcg aggacctttg ccgcgcccgg gagctcggcg tgaacgcggt
     2941 gctgataccg agctacgacg agctctatcc cgatgacttc cggtttcgag tcgaggaaca
     3001 cgagttcagc cgtgagcttt gcggggcgca tcgtccgggg cacttcactg gcgtgatgac
     3061 cgtggtgctc aagctcctca accttgtccg gccgcggcgc gcgtacttcg gcgaaaagga
     3121 ttatcagcag tatcttctga tacgtgacat gtgcgatgcg ttcttcgtgg atgtcgaaat
     3181 cgtgccatgt gccacggtac gcgaggagga cgggctggcg ctgagttccc gaaacgcgtt
     3241 gctggacgag aaaggccgcg cgcgtgctgg tcggctgaat cggctgctgc gcgacgcgcc
     3301 gagcgatgct gctgcttccc gggctctcga gcaggcggga ttcgaggtgg actacgtggt
     3361 caccaaagcc gatcggcgat tcgccgccgc cactttgcgc tgcggcgaaa gagaagtccg
     3421 actgatcgac aacgttgcga tcgcgcagca cggctaggcc gaggaggagg catgctgctc
     3481 tgtttggatg tgggtaacag ccagatctac tgcggcgtct acgatggcga ccgcctcacc
     3541 gtgcagtttc gacgaacctc tacggtgcgt agctcctcgg acgagctcgg agtgtttctg
     3601 cgcggcgcgc tgcgagaaaa cggcgtgtca cccgacggga tcactcagat cgccatctgc
     3661 tccgtcgtgc ccgacgttct ctactcgctg cgctcctgct gccagaaata ctttggcctc
     3721 gagccgctga tcatgcgccc gggcatcaag acgggcctca agatctccta caaggatcca
     3781 aaggaggtgg gcgcggaccg gatcgccgat gccattggcg ccatcaagct ctatccgaat
     3841 cggaacctca tcgtggtgga ttttgggact gcgaccaccg tctgcgccat ctcccgggac
     3901 cgtgagtttc tcggaggcaa catcgtgccg ggcgtccgac tggcaatgga tgccctggaa
     3961 gagaaaaccg cgcagctgcc tagcgtggag atattcccgg tgacctcggc tatcgggcgc
     4021 tccacggtgg agagcattca gaacggtctt tactggagca atgtcggcat gatcaaggag
     4081 ctggtagccc gagtgaccga ggaagcgttc ccggacgatg cgccggtcgt gatcgcgacg
     4141 gggggcttcg cgacgctctt tgacgggctg gggctcttcg acgccattgt ctccgatctc
     4201 atcctgatcg gcctgcgtga agcggtccgg ctgaacgcct gacctgaaaa ccctgcaggc
     4261 gttcatgcgt cgttttatga ctcccccctg ttatgctcgc ggttatgagc atgccgcagc
     4321 cccgatttgc tgacgccagg caccgacagt ttgcactggg actgcttggc gtttacctgc
     4381 tcatctggac gtccctggcc atcaagccgc tgtatcgggc ggactggctt ctggaaaacg
     4441 tcctggtggc catcgcgctg cccatgctgg tggcgaccta tcacaggctg cctctgtcca
     4501 gaatctcgta cgcgatgctt ttcatcttct tctgcattca cgccgtcggt tcgcactaca
     4561 cctatgccga agtgccctac gacgactgga cccgccggct gttcggcacc agcctcggtg
     4621 aagcagccgg ttgggatcgc aaccactacg atcgctttgt ccacctgatg tacgggctgc
     4681 tcatcgttta ccccgtacga gagctcttcc tgcgcgtcgc ggatgccaga ggtttctggg
     4741 gatatttttt cccgcttctg gtggtcatgt cgtcctcgct ggcctacgag ctgctggagt
     4801 ggggggccgc cttcgtgttc ggcggcgagt tgggtatggc ctatctcggc acccagggtg
     4861 acgtctggga cgcccacaag gacagccttc tggcaaccgg cggcgcgctg gccgccaccc
     4921 tggtgattgc cgccgtccac tcatccttcg accgggactt cggccgggat tggtcgcaga
     4981 gcctgactgt caagaatccc gatcccttgg gcgaggcagc catcgggcgt tatctgaacg
     5041 aacgggagaa tgccccatga gtccattgtt gccgagtgcg ggcggagggc tgaagctgtt
     5101 cagcatcttc ggcatcgaga tccggctcga ttacagcgtt gccatcatct tcggcctcgt
     5161 cgtgtacagc ctgggaggcg ggctgtttcc ccagtggcat cccgactggc cgccctcgac
     5221 ggcgtggctg acggcgctgg tggccggcgt gctcttcttc gcatcgttgc tggcccacga
     5281 gctttctcat tcgctcacgg cccgccgata cgggataagg gtgccgagaa ttaccctgtt
     5341 cctgtttggc ggcgtagccg agatcgagtc ggaggcgcaa accccgtcag cagagctgac
     5401 catagccatt gcgggtccgt tgatgagcgc tctgctgggc gcggtgttca cggtggttgc
     5461 gtttgggagc atggacaccc tggcgaggga agcgtttgcg gaagatccgg aaaccgtcat
     5521 cgccagcctg agcccgggat acaccgcctg tctgtggctc ggctccgtga acatgatgct
     5581 ggccgtgttc aacctgattc ccggtttccc gcttgatggc ggcagggtgt ttcgtgccat
     5641 tatctggaag ctgaccggaa accagctcag ggcaacacgg ctggcaagta ccaccggtcg
     5701 ctggttcggc tgggccatca tgggcatcgg cctgtggaac ctgctggtgc tcaaatccgc
     5761 tggcgggctc tggctgctgc tcatcggctg gtttttgagc catctcgcag cggccagtta
     5821 ctcccaggcc ctcgctcagc agacgctcgc tccccttcgg gtccgcgacg tgatgcgcac
     5881 tcgcttcgag ttcgcaccgt cgggcatcac gatcgatgag ttcatcaacg actacctgct
     5941 caggagcaat cagattctct ggccgcttca gggccccgcg ggagtggttg gagccgtcaa
     6001 cttcgagcag gttgccagcg tgcctccgga tcagcgcgca accaggctgg tccaggaggt
     6061 gatggtgccg gtggacaggc tgcctgcgct cgatgccgac gatctggcga ccaatgccct
     6121 gaacacgctg ctcggtgccg gagatcaacc ggttccggta gtgagcggtc agcggatcgt
     6181 cggtctggtg cgcggctcgg acatcctgaa atgggtgatg cttcaccagg gtggcactcc
     6241 gtcgtgatca cgttcccgtc ttcggtcttc gtcattgagc gcgcgagggg gcagcttcgc
     6301 cttccccgcc gggttttagc ggctcgatct tgaaccccgc gtagccgtag gcggccgaga
     6361 cgaacgggtt ggccagattg aagaaacaga acggcagata ggccagggta ggtacgccca
     6421 gcgtcgctgc catgtaggcg ccgcaggtgt tccatgggac gagaggcgag gtgatggtgc
     6481 cgccgtcttc gagggtccgg gcaaggtttt tctcgtgcag cccgcg
//
LOCUS       DBNHOX010000089         3537 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_521391, whole genome shotgun sequence.
ACCESSION   DBNHOX010000089 DBNHOX010000000
VERSION     DBNHOX010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_521391"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1418)
                     /locus_tag="AC16UZ_02010"
     CDS             complement(135..1418)
                     /locus_tag="AC16UZ_02010"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6245483.1"
                     /translation="MNDPESIANLEARMAWEQERAVPPEGFPALPPLPPGRYTSPAFF
                     DLERRHLWSKVWLYAAHDSQLPEPGSFLLLDIPDAPIFLIRGRDASVRAFYNVCSHRG
                     APLVREASGQVARLRCTYHQWTYDTDGRLIAVMDERDFAEPFDRSCLGLKEVRCEQWG
                     RWIFINEDPEAQPLLEWLDMIAAEFEQFDIERLRPAAIKSYELECNWKLTMDAFLEVY
                     HIQGIHPKTVGPSLDHRGAVMGLLPNGHTRMTCPTRMSRTETADTRGYTDSAGEAMPA
                     IPAGEIARNYHVSHNVFPNLITPVGDTARQFLMFWPLSLTRTRVDVVHFGLDWGEGER
                     PSGWDGLLAFWDVVMAEDLQFLEWQQKAVLSPGFQGYRLSYMERRIYYAHESIDRIIG
                     VENIPEALRAVPMLDAYRERAEDRCADQPSSPSSR"
     gene            complement(1442..1927)
                     /locus_tag="AC16UZ_02015"
     CDS             complement(1442..1927)
                     /locus_tag="AC16UZ_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6245484.1"
                     /translation="MNFDDYHAIVRRRYEYALGIDTRDWVLYRSIFTDEITMDFSSYN
                     GRPASGMAADDWVAGCKVLFAGLDATQHVMSNPMVDVDGDNARLRMYMKAEHFLRNDQ
                     GGMDFTIGGYYDDRLVRTDEGWKMQAVTLNVFWSRGNRQIMALAAEIGKGKLEEPSSG
                     S"
     gene            complement(1942..2673)
                     /locus_tag="AC16UZ_02020"
     CDS             complement(1942..2673)
                     /locus_tag="AC16UZ_02020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6245485.1"
                     /translation="MNDNAIALVTGGATGIGAEVCRQLAAAGARVAICDVNEADGAPL
                     AESLGGEFLFCDVADLASVEGAVAGCCARLGVPDYVHLNAGVMTVGRYDAYLALEDVS
                     VEQYRRIIGVNVDGVFNGIKALLPPMRQKGGAITVTASIAGFAPMAIDPLYGATKAAL
                     ISLVRSVAAANEGGSVRINAICPGVVDTAIVPDAMSGTIPMMPPSAMAAEILDLLSDG
                     PSGEIRVKLNEETPAFHVEPSTLGR"
     gene            2723..>3537
                     /locus_tag="AC16UZ_02025"
     CDS             2723..>3537
                     /locus_tag="AC16UZ_02025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6245486.1"
                     /translation="MDHHRPNVTTTIDRSPTRPDKAADTTLKALFLLTAVLMVGYGAV
                     FSLLAEIRDAFGFTPTGIGLIAGSAFAAGFFAQLWLARYADRGYGAIMLRAGLAISAL
                     STLWMAVADTLAAWIAARALLGFGAGCVRPAVRRLALTADPANAGHAMGRLTAYETAG
                     FLLGPVLAALLDRWAGLASTFIVMTGVLALLAPMVWRVSIPGASRPTTGPVIGTLLRN
                     PYMQACLALGIAFWITIGVFEAVWAIFLADLGASQMFIGLTMSLFGIPRIFIAP"
BASE COUNT          580 a         1191 c         1124 g          642 t
ORIGIN      
        1 cgctgaagct catctggtca tacccgctca ctcccgttcg gcacgcccgg ctgcgggagc
       61 ggatcgagcg gcgtaacgcc aggcgcacgg cacggtaggg atcaccgggc ccctcgggca
      121 cgggaacccc ggggttaacg ggaggacggg ctcgacggct gatccgcgca gcggtcttcc
      181 gcgcgctcgc ggtaggcatc cagcatgggc acggcgcgca gcgcctcggg aatgttctcc
      241 acgccgatga tccggtcgat ggactcgtgg gcgtagtaga tccggcgttc catgtagctc
      301 aggcggtagc cctgaaatcc tggcgagagc acggccttct gctgccactc cagaaactgc
      361 agatcctcgg ccatcaccac gtcccagaag gcgagcaggc catcccatcc cgagggacgc
      421 tcgccctcac cccagtccag gccgaaatgg accacgtcca cccgggtccg cgtgagcgaa
      481 aggggccaga acatcagaaa ctgcctggcc gtgtcgccaa cgggcgtgat gagattcgga
      541 aagacgttgt gggagacgtg atagttgcga gcgatctcgc cagccggaat ggcaggcatc
      601 gcctcgcctg ctgaatcggt gtatccgcgc gtgtcggcgg tctcggtgcg ggacatccgg
      661 gtcgggcaag tcatgcgcgt gtgcccgttg ggaagcagcc ccatgacggc gccccggtgg
      721 tcgagcgatg gccccacggt cttcgggtgg atgccctgta tgtggtagac ctccaggaac
      781 gcatccatgg tcagcttcca gttgcactcg agctcgtagc tcttgatcgc cgccgggcgc
      841 aggcgctcga tgtcgaactg ctcgaactcg gcggcgatca tgtcgagcca ttccagcagc
      901 ggctgtgctt cggggtcctc gttgatgaat atccaccgac cccactgctc gcagcgaact
      961 tccttcaggc cgaggcagga tcgatcgaag ggctccgcga agtcgcgctc gtccatgacg
     1021 gcgatcagtc gtccgtccgt gtcgtacgtc cactggtggt aggtgcagcg aagccgggcc
     1081 acctgaccgg aggcctcccg caccaacggc gctccgcgat gcgagcagac gttgtaaaac
     1141 gcgcgaacgc ttgcatcgcg gccacgaatc agaaagatcg gcgcatcggg gatgtccagc
     1201 aggagaaagc tgccgggctc cggcagctgg ctgtcgtggg ccgcgtagag ccagaccttt
     1261 gaccacaggt gacgacgctc gagatcgaaa aatgccgggc tggtgtagcg gccgggcggc
     1321 agaggcggca acgccggaaa gccctcgggc ggaaccgccc gttcctgctc ccaggccatg
     1381 cgcgcctcga ggttcgcaat ggactccgga tcgttcatcg gccgtactcc ctaacgcgct
     1441 gtcacgaacc cgacgacggt tcttcgagct tgccctttcc aatctccgcc gcgagcgcca
     1501 tgatctgccg attgccgcgg ctccagaaga cgttcaacgt caccgcctgc atcttccagc
     1561 cctcatccgt cctgaccagc cggtcgtcgt agtagccgcc gatggtgaaa tccatgccgc
     1621 cctgatcgtt gcgcagaaag tgctcggcct tcatgtacat gcgcagccgg gcattatccc
     1681 cgtcgacgtc gaccatcgga ttggacatca cgtgctgggt ggcatcgagc ccggcaaaca
     1741 gaaccttaca gccggccacc cagtcgtcgg ccgccatgcc ggatgcgggt cggccattgt
     1801 aggaagagaa gtccatggtg atctcgtcgg tgaagatgct gcgatagagc acccagtccc
     1861 gagtgtcgat ccccagcgcg tactcgtagc ggcgacggac gatggcatgg taatcgtcga
     1921 aattcatgat ggcctgcccg ttcagcgacc caaggtgctc ggctcgacgt ggaaggcggg
     1981 cgtctcctcg ttgagcttga cgcggatctc gccggagggg ccatcgctga ggaggtccag
     2041 tatctcagcc gccatggcgg acggtggcat catcggtatc gtgccggaca tcgcatcggg
     2101 cactatggcg gtgtccacca cccccgggca gatggcgttg atacgcacgc tgccgccttc
     2161 gttggctgcc gcgacgctgc gcacgaggct gatcagggcg gccttggtgg cgccgtagag
     2221 cggatcgatg gccatcgggg cgaagccggc aatggaggcc gtcaccgtga tggctccgcc
     2281 cttctgcctc atgggaggca gaagcgcctt gataccgttg aagacgccgt ccacgttgac
     2341 gccgatgatc cggcggtact gctccacgct gacgtcctcg agcgcaaggt aagcatcgta
     2401 gcggcccaca gtcatgacgc cggcattcag gtgtacgtag tcgggcaccc caagccgggc
     2461 acagcagccg gcgaccgctc cctccacgct cgccaggtcc gccacgtcgc agaacagaaa
     2521 ctcgccgccc agggactcgg ccagcggcgc gccgtccgcc tcgttgacgt cgcagatggc
     2581 cacccttgct cccgccgcgg cgagctgtct gcacacctcg gcgccgatac cggttgcccc
     2641 tccggtcacc agcgcgatcg cgttgtcgtt cacccttccc ctcctcgcat gatctttgct
     2701 ttccgcccta gattgcggtt cgatggacca tcaccgacca aacgttacca cgacgatcga
     2761 ccgttcacct accagacctg acaaggcagc cgataccacg ctcaaagccc ttttccttct
     2821 gaccgccgtc ctgatggtgg gctacggggc ggtgttttcc ctgctggccg aaatccgcga
     2881 cgcgttcggc ttcacgccta cgggcatcgg gctgatcgca ggctctgcgt tcgcggccgg
     2941 tttcttcgcc cagctgtggc ttgcgcggta cgcggatcgc ggttacgggg cgatcatgct
     3001 gcgggcaggt ctcgcgatct cggcgctgag caccctgtgg atggccgtcg ccgacaccct
     3061 cgctgcgtgg attgccgcgc gcgccctgct ggggttcggc gccggctgcg tccgccccgc
     3121 cgtacgtcgt ctggcactca ccgcagaccc cgccaatgcc ggccacgcca tgggacgttt
     3181 gaccgcctac gagacggctg gcttccttct cggccccgtg ctcgccgcgc tgctcgaccg
     3241 ctgggctggc ctggcgtcca ccttcatcgt gatgacgggc gtcctggcgc tgctcgcgcc
     3301 catggtgtgg cgcgtgagca ttcccggggc cagccggccg acaacgggtc ccgtcatcgg
     3361 cacgctgttg cgcaatcctt acatgcaggc atgtcttgct ctcggcatcg cgttctggat
     3421 caccatcggc gtcttcgaag cggtctgggc catatttctc gccgatctgg gggcgagcca
     3481 gatgttcatc gggctcacca tgagtctctt cgggatcccg aggatcttca tcgcgcc
//
LOCUS       DBNHOX010000090         2044 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_264155, whole genome shotgun sequence.
ACCESSION   DBNHOX010000090 DBNHOX010000000
VERSION     DBNHOX010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_264155"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="AC16UZ_02030"
     CDS             complement(<1..266)
                     /locus_tag="AC16UZ_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245487.1"
                     /translation="MSTRILMLSMLLLAGCASAPEAEQAQQQREEDIATILNEPLDPS
                     EYGEAKRCLAGHEYRDFAIIDDRRIVFKGPGNSLWLNELRARCP"
     gene            complement(332..1234)
                     /locus_tag="AC16UZ_02035"
     CDS             complement(332..1234)
                     /locus_tag="AC16UZ_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR6245488.1"
                     /translation="MTTEQLLRFATYASVATATVLIAAKLTAWFATDSVSVLASLVDS
                     VMDVGASLINLFAVRYSLMPADQEHRFGHGKAEALAGLGQATFIAGSAIFLILHAVDR
                     LMDPVTIEHVAAGSAVMGFAIVMTLVLLTIQRHVIRRTGSTAIRADALHYATDLATNA
                     ATLLALLLAAYGFSGLDPLFGLAIAAYILYSASRIGRDAVSLLMDRELPQAEREEIKR
                     VVDGTPGVLGVHGLRTWQSGQRKVIQMHLELDPDLPLREAHRIAVEVERRLLEHEPEA
                     DITIHQDPAGQPSHVAHNPGDAFS"
     gene            complement(1329..1580)
                     /locus_tag="AC16UZ_02040"
     CDS             complement(1329..1580)
                     /locus_tag="AC16UZ_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245489.1"
                     /translation="MVAMMLIVTGVLGTMTHMMPGCTRRCFAGPLAGTSQRRERRRQH
                     KRYQQMRNKGIHEAHERRLSRDPPLHNAYTVPLPTGSSD"
BASE COUNT          341 a          613 c          705 g          385 t
ORIGIN      
        1 tcggggcagc gagcccgaag ctcgttcagc cacaggctgt ttccaggacc tttgaacacg
       61 atgcggcggt cgtcgatgat ggcgaagtcg cggtactcgt ggcccgccag gcagcgtttg
      121 gcctcaccgt actccgacgg gtcgaggggt tcgttcagaa tcgtggcaat gtcttcctcc
      181 cgctgctgct gggcctgctc ggcctcgggc gccgacgcgc agccggcgag cagcagcatg
      241 gagagcatta gaatccgggt gctcatggcg acgaacgtta gcccgaaacg cggtcgactg
      301 acaaatcgcc ccgctgacct catcggatga gtcaactgaa cgcgtctcca gggttgtgag
      361 caacgtggga tggctgtccc gccggatcct gatggatggt gatgtcagcc tccggctcgt
      421 gttcgagcag gcgtcgctcc acctccacgg cgatgcggtg agcctctctg agcgggaggt
      481 ccgggtccag ctccaggtgc atctggatga ccttgcgctg tccggactgc caggtgcgca
      541 gcccgtgaac gcccagcacc ccaggcgtgc cgtccacgac tcgcttgatt tcctcgcgct
      601 cggcttgggg cagctcccgg tccatcagca ggctgacggc gtctcgaccg atgcggctgg
      661 cggagtaaag gatgtaggct gcgatggcca ggccgaacag cggatccaac cctgagaaac
      721 cgtaggccgc cagcaacagc gcgagcaggg tggctgcgtt ggtggccagg tccgttgcgt
      781 agtgcagcgc atccgcgcgt atggcagtcg aaccggtgcg gcggattacg tgccgctgaa
      841 tcgtcagcag caccagcgtc atgacgatgg cgaatcccat caccgccgag cctgccgcga
      901 cgtgctcgat cgtgacggga tccattagtc ggtctactgc gtgcagtatc aggaatatgg
      961 cggagccggc gatgaaggtc gcctgaccca gaccggccag cgcctcggct ttgccatgcc
     1021 cgaagcggtg ttcctggtcg gcgggcatga gcgagtagcg cacggcaaac agattgatca
     1081 gcgatgctcc cacgtccatg accgaatcga ccagtgaggc caggacgctc accgaatcgg
     1141 tggcgaacca ggcggtcagt ttcgccgcga tgagcaccgt ggctgtagcc acggatgcgt
     1201 aggtcgcaaa gcgcagaagc tgttcggtgg tcatggcggg catggccagg cagatcgggt
     1261 ggccggagat gttacaccac gaccggttgc gacggggcct cgggcaggtg ttttgaccat
     1321 cctcttgctc aatcagagga accagttggc aacggcacgg tgtaggcgtt gtggagcggg
     1381 ggatctcgcg atagccttcg ctcatgagcc tcgtgaatgc ccttgttgcg catctgctgg
     1441 tagcgcttat gctgacgacg ccgctcacgg cgttggctgg ttcctgcgag ggggccggcg
     1501 aagcacctgc gcgtgcagcc gggcatcatg tgggtcatgg tcccgagcac gccggttacg
     1561 atgagcatca tggcgaccat ggggaggtgt ccgtcgaaga cggggccgcg gactgtgatt
     1621 gctgcgacga gtgtctccag gagtgtgccg cctcgggatc gacgtccttt ctcgccggga
     1681 gtgctctgga agcgccactg cgatccggtc cgaaacggtt cgtcaccgac tggctgagcg
     1741 gtcacgtccg cgcgcctccc acgtccttat tccgtcctcc aatcctcgac tgctgatcga
     1801 gcggcgctgc tggcgcccca gttcccatgg cccgttgcgg tcttctggga gtcggcatgg
     1861 tgccgctcat ttctcggtca tcagtgaact gaacgagggt gtggcggatg aaacgacgaa
     1921 tgatgaggac gtggctggtc gcgtccctgg cggttgcggg tgttgcccaa ggctccgagg
     1981 acggcggcga tctgctccgg acgggtttga cgctggagct ggccctggag agagcggtgc
     2041 gcac
//
LOCUS       DBNHOX010000091         2564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1164298, whole genome shotgun sequence.
ACCESSION   DBNHOX010000091 DBNHOX010000000
VERSION     DBNHOX010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1164298"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="AC16UZ_02045"
     CDS             complement(<1..1001)
                     /locus_tag="AC16UZ_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6245490.1"
                     /translation="MRIIVIVACVAIVLAGALFGAGLYFSAPVFDERVSEPPDGAFDI
                     LPVSEALTFARTKDGRLLLVRSADADAIEAVDLGAALDTVATDPLAILRTHGYQRVAA
                     RLTGVAQAVPLEQLGMPFDPGYPHIAAGTNFRAHAEEVGLDEGPFLFPKLTRATAWDA
                     RVPARTRLDYEVELCAVALGVYSADGPAPLGFVLCNDFTDRWALLKEIDLDTPMGQSG
                     FPDAKGGAGMLPIGPFLLVPSRDADFHRRLEIGLAVNDRLRQRDSAGLMIWSPHEIAL
                     TALENCEAPFVSTAGSHRLTPCDAIDEATLLLTGTPAGVAFHFLNIWRPGSYLRSGDR
                     "
     gene            complement(1009..1491)
                     /locus_tag="AC16UZ_02050"
     CDS             complement(1009..1491)
                     /locus_tag="AC16UZ_02050"
                     /inference="COORDINATES: protein motif:HMM:NF042724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6640 family protein"
                     /protein_id="HIR6245491.1"
                     /translation="MPRSFRAELMPVDNPFDQNLIPRILMSLMALAIAIGPMFADFNK
                     THATNPLWPPHARFHVVWQVLTQAGISVVALVLLWRASPDYVLHLYLAAALIYVWLLT
                     FFMTLASIKVYQGALRDVNGIEPFRFNILGKVREVDTNLFGVVLFTALNTVAALLIRG
                     "
     gene            complement(1469..2137)
                     /locus_tag="AC16UZ_02055"
     CDS             complement(1469..2137)
                     /locus_tag="AC16UZ_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6245492.1"
                     /translation="METFFRQQAIGFNKRLRTRHALIDSAIDVFSEKGFEDASISEIA
                     AVAGLANGTFYNHFRDKDELAAASAAAITLEIAKQLDERMSDLEAGVSRVVAASFAFL
                     RLASEHKPWGMVLVEQFQRRPGEAWAAMQYMRADIQLALEQRGLDVVLDDFLLEQLGA
                     LMIAALRRQLSEGLQEAQLQRTCEHVLRVLGLTPEEARREVEGVAGHVLLGDELSLAE
                     IFPR"
     gene            2219..>2564
                     /locus_tag="AC16UZ_02060"
     CDS             2219..>2564
                     /locus_tag="AC16UZ_02060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6245493.1"
                     /translation="MLSTQVLIIGAGPVGLLSAIVLDRLGISSVLVERRLDRLQAPKA
                     HAVNPRTLEICQRFGVPADEVRAAGASIEDGGQVHFVDVLNGTCFGSLPYERQDDGAR
                     AFTPWPLVNIPQP"
BASE COUNT          515 a          816 c          806 g          427 t
ORIGIN      
        1 cgatcgcccg atcgcaggta agagcccggc cgccatatgt tcagaaaatg aaacgcgaca
       61 ccggccggcg tcccggtaag aagcaacgtc gcctcgtcaa tggcatcgca cggcgtgagc
      121 cggtggctgc cggcggtgct gacgaagggc gcttcgcagt tctcgagcgc ggtcaaggcg
      181 atctcgtggg gcgaccagat catcaggccc gcggagtcgc gctgccgcag acgatcgttg
      241 accgcgaggc cgatttccag gcgccggtga aagtccgcat cgcgactggg caccagcagg
      301 aatggcccga tgggcagcat gcccgccccg cccttggcgt cgggaaatcc ggattggccc
      361 atcggcgtgt ccaggtcgat ttccttcagg agcgcccagc gatcggtaaa gtcgttgcac
      421 agcacgaaac ccagcggggc aggcccgtcg gcggagtaga cgccgagggc aacggcgcag
      481 agctcgacct cgtaatccag gcgcgtacgt gccggtaccc gtgcgtccca cgctgtagcc
      541 cgggtcagct tgggaaacag gaagggcccc tcgtccaggc ccacttcctc ggcatgggcg
      601 cggaaattgg tccccgcagc gatgtgcggg tagccggggt cgaacggcat gccgagctgc
      661 tccagcggaa ccgcttgggc cacgcctgtc agccttgccg ccacccgctg gtagccgtgt
      721 gtgcgcagaa tggccaacgg gtctgttgcc accgtgtcga gcgcggcgcc gaggtcgacg
      781 gcctctatgg catccgcgtc ggcgctgcgc acaagcagca gccggccatc cttcgtgcgg
      841 gcaaaggtca gcgcctcgct gacgggcaga atgtcgaagg caccatcggg gggttctgag
      901 acgcgctcgt cgaaaaccgg tgcagaaaaa tagaggccgg caccaaagag cgccccagcg
      961 agcacaatcg ccacgcaggc gacgatgacg atgatcctca tgactcgttc acccccggat
     1021 cagcagcgcg gcaaccgtgt tcagcgcggt gaaaagaacc acaccgaaga gattcgtgtc
     1081 cacctcgcgc accttgccca atatgttgaa ccggaagggt tcgatgccgt tcacatcccg
     1141 aagcgcgccc tggtaaacct taatcgaggc gagggtcatg aagaaggtga ggagccagac
     1201 atagatgagc gcggccgcga ggtagaggtg caacacatag tcaggggacg ctcgccacag
     1261 cagcaccagg gcgaccacgc tgataccagc ctgggtcagc acctgccaga ccacgtgaaa
     1321 gcgcgcatgt ggcggccaca gcgggttcgt ggcgtgggtt ttgttgaaat cggcaaacat
     1381 gggaccgatc gctatggcca aggccatcag gctcatgagg atgcggggaa tcagattctg
     1441 gtcgaacggg ttgtccacgg gcatcaactc agcgcggaaa gatctcggca agactcagtt
     1501 cgtctccgag cagcacatgc ccggcgacgc cctccacttc gcgcctggcc tcctcgggcg
     1561 tcagccccag aacgcgcagg acgtgctcac aggtgcgctg taactgagcc tcctgcaagc
     1621 cctcagagag ctgccttcgc aaggcggcga tcatgagcgc gccaagctgc tcgagcagga
     1681 aatcatcaag aaccacgtcg agaccacgct gctccaacgc cagctgtatg tccgcccgca
     1741 tgtactgcat ggctgcccac gcctcgccag gacggcgctg aaactgctcc accaggacca
     1801 tgccccaggg tttgtgctcc gaagcgagcc gaagaaaggc aaacgacgcc gccacgacgc
     1861 gcgacacccc tgcctcgaga tcgctcatgc gctcgtcgag ctgcttggcg atctccagcg
     1921 tgatggccgc cgcggaagcc gcggccagct cgtctttgtc cctgaaatga ttgtagaaag
     1981 tgccgttggc cagacccgcg acagcggcga tctcgctgat gctcgcatct tcgaaaccct
     2041 tctccgagaa cacgtcaatg gcactgtcga tcagcgcgtg acgtgttcga agtcgcttgt
     2101 tgaaaccgat ggcctgctgc cggaagaaag tctccatggc tcggcatcct aggatcaatg
     2161 aattgacatg tcaatcatat ttgtatttac tctcattttt taacgaacac tcatatccat
     2221 gctgtcaacc caggttctca tcatcggcgc cggaccggtg ggcctcttga gcgcgatcgt
     2281 tctcgaccgg ctggggattt ccagcgtgct cgtggagcga cggctcgatc gccttcaggc
     2341 gccgaaggca cacgcggtca atccgcgcac cctggagatc tgtcagcgct tcggggtacc
     2401 ggccgacgag gtgcgcgcgg ccggcgcgag cattgaggac ggcgggcagg tgcacttcgt
     2461 ggacgtgctc aacggcacct gtttcggaag cctgccctac gaacgccagg acgatggggc
     2521 cagagcgttc acgccttggc cgttggtgaa cattccccaa cccc
//
LOCUS       DBNHOX010000092         2280 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1035877, whole genome shotgun sequence.
ACCESSION   DBNHOX010000092 DBNHOX010000000
VERSION     DBNHOX010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1035877"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="AC16UZ_02065"
     CDS             <1..684
                     /locus_tag="AC16UZ_02065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6245494.1"
                     /translation="EMWDRPELSRRDRSLMVIAVLAAQARDEELTLHTRIGMRHGLSR
                     TEIEEINLHVAAYAGFPAAMAAQRRMDQAFREAQGVEHIDDREGAEHLGDDERLRRAA
                     DVRRTLAGAEAALDPEADLASLRDYLGGVGEWAMKWAFGEVWSRPQLSRRDRSLVVVA
                     ILTALGQEAELAFHIPWGLNHGLTRAEVEEIMTHLCLYTGFPRAVEGMRAAKHAFAEL
                     DGQATAQPQ"
     gene            717..1943
                     /locus_tag="AC16UZ_02070"
     CDS             717..1943
                     /locus_tag="AC16UZ_02070"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6245495.1"
                     /translation="MIDVDAHLHEPLDWIEQTDPALARELGPPARFMDVANSVFGIAN
                     PALAELSEGQPPSGGYETILPGFVHHLEMTDQRQPERQCGSDDDPFCGAEARLAFCDE
                     RGIDVQFLNPTFLVGAFVQAARARRFELMGEIRQCWNRWAADQVHGHADRLIPVTQID
                     LSNVPRSIEEMTRMRERGSRAFAIPESPAGKPRFARNGEPALAKSLTHPDFEPLWSAA
                     EDLGMAAIAHVGFSRERINPGWANNGADDLMTYSFLNMVVNPQLAPQLLLAAMAFDGV
                     FERHPKLTVIVEEVGIAWLPPLLDALDQGVGRAGHRALEDGAYRPDFAGSAYNLPLSP
                     SEYLRRQVRVTPLVASQPLHPTIDLVPEMLCFSSDYPHVEGSGEAMAVCERQLGTLSD
                     EAHRAFYAGVGEMIGL"
     gene            2049..>2280
                     /locus_tag="AC16UZ_02075"
     CDS             2049..>2280
                     /locus_tag="AC16UZ_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6245496.1"
                     /translation="MEYDEVVLGRRSIRGFSKEPVPKALIREVVEIAMRAPSSLNTQP
                     WHFHVVSGEPLDRIREGNTERNLAGVPDSREFR"
BASE COUNT          384 a          766 c          756 g          374 t
ORIGIN      
        1 gagatgtggg accggcccga gctcagccgc cgggatcgca gcctcatggt catcgccgtg
       61 ctggcggccc aggcccgcga cgaggagctg actctgcata cccgcatcgg gatgcgacac
      121 gggctcagcc gcaccgagat cgaggagatc aacctccacg tcgccgccta cgcgggcttt
      181 cctgcggcca tggccgcaca gcggcgtatg gatcaggctt ttcgcgaagc ccaaggcgtg
      241 gaacacatcg acgaccgcga gggagcggag catctcggcg acgacgagcg gctgcgtcgg
      301 gcggccgacg tgcggcgcac gctcgccggc gccgaggctg ccctcgatcc ggaagccgat
      361 ctggcgtcgc tgcgcgacta tctcggcggc gttggcgagt gggcgatgaa atgggcgttc
      421 ggggaggtct ggtcgcggcc gcagctctcc cgcagagacc gctccctggt ggtcgtcgcc
      481 atcctgaccg ccctcggcca ggaggccgag ctcgcctttc atatcccctg gggtctaaac
      541 catggcctta cgcgcgctga ggtcgaggag atcatgaccc acctgtgcct gtacaccggg
      601 tttccaaggg cagtcgaggg catgcgggcc gccaagcacg ccttcgcgga gctcgacggg
      661 caggccacgg cacaaccgca atgactcgtg actctggggg gattcacgcg atccgcgtga
      721 tcgacgtcga cgcccatctc cacgagccgc tcgactggat cgagcagaca gaccccgcgc
      781 tcgccaggga gctggggccg ccggcacgct tcatggacgt ggctaattcg gttttcggga
      841 tcgcgaatcc cgctctcgcc gagctgtccg aggggcagcc cccctcgggt ggctacgaaa
      901 ccatcctgcc tggcttcgtg catcacctgg agatgaccga ccagcgccaa ccggagcgtc
      961 agtgtggatc agacgacgac cctttctgcg gagccgaggc gcgcctggcg ttctgcgacg
     1021 aacgaggcat cgacgtccag ttcctgaacc ccacgttcct ggtgggagcg ttcgtccagg
     1081 cagcacgcgc acgacgcttc gagctcatgg gcgaaattcg ccagtgctgg aaccgatggg
     1141 cggccgatca ggtgcacggc cacgcggacc ggctcattcc ggtcacacag atcgacctgt
     1201 ccaacgtccc gcggtccatc gaggagatga cgcgcatgcg cgagcgcggc agccgggcgt
     1261 tcgcgatccc ggagtctccc gcgggcaagc cgcgattcgc acgcaacggc gagcccgcgc
     1321 tggccaaatc gctgacccac ccggatttcg aaccgctgtg gtcggcggcc gaggacctgg
     1381 gcatggcggc catcgcccac gtcggatttt cccgggaacg catcaacccc gggtgggcca
     1441 acaacggtgc cgacgatctg atgacctaca gctttctgaa catggtggtc aacccccagc
     1501 tcgctccgca gctgctgctg gccgctatgg ccttcgatgg cgtgttcgag cgacacccca
     1561 agcttactgt gatcgtggag gaagtcggca tcgcctggct gccgcctctg ctcgacgccc
     1621 tggaccaggg cgtggggcgg gccggtcacc gggcgctgga agatggcgcc taccggccgg
     1681 actttgcggg atccgcgtac aacctgccgc tctcgccctc cgagtacctg cgccgacagg
     1741 ttcgcgtgac cccgctggtg gcctcgcaac ctctacaccc caccatcgac ctggttcccg
     1801 agatgctctg cttctccagc gactatcccc atgtggaagg ttccggcgaa gccatggcgg
     1861 tgtgcgagcg acagctggga accctgagcg acgaggccca ccgggctttc tatgcggggg
     1921 tgggggagat gatcgggctc tgacgcgccg tcgtcgcagc tctggatgcc cgtatgccca
     1981 gcctggacgg gagctggtca acccggcaaa cgaggtagaa tccgagcttt ctcactcagg
     2041 agccatcgtt ggaatacgat gaagtcgttc tcggccgccg gagcatccga ggcttcagca
     2101 aggagccggt tcccaaggcg ctgatacgcg aggttgtgga aatcgcgatg cgagcgccct
     2161 cctcgctcaa tacccagccc tggcactttc atgtggtcag cggagagccg ctggaccgga
     2221 ttcgcgaagg caataccgag cggaaccttg ccggcgtgcc ggattccagg gagttccgcg
//
LOCUS       DBNHOX010000093         4822 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1486063, whole genome shotgun sequence.
ACCESSION   DBNHOX010000093 DBNHOX010000000
VERSION     DBNHOX010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1486063"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="AC16UZ_02080"
     CDS             <1..768
                     /locus_tag="AC16UZ_02080"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6245497.1"
                     /translation="ATHYLSAGMNVLIADLDEDALSTAAASLADSGSGTLHTQVCDVS
                     DFAQVETLQATAAAKLGNVHCLMNNAGAGFPPSAPWENLDVWKKQVEINLWGIIHGCQ
                     AFVPAMLEAGERGAIVNTGSKQGITNPPGGYAYNLSKAGVKNYTESLAHALRQVEGCQ
                     LSAHLLVPGFTYTGMISRFIPEKPAGAWTPDEVVAFLIRSLDRGDFYVLCPDNDTPRD
                     LDEKRIQWNTDDLIRNRSALSRWDPKYKDAYEAFVSD"
     gene            1144..4152
                     /locus_tag="AC16UZ_02085"
     CDS             1144..4152
                     /locus_tag="AC16UZ_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245498.1"
                     /translation="MGRNGEGRAPRGTRGFQAGCLSALLFLGACTGSSEKIFEQSEEL
                     SLRGSVGDGPIVGAQIVVEDAEGQMVFTAESDAAANYQIDVPDGTDLPVTIRVSGGTD
                     LVTGRGADFELLAVAFTSGDLTVNASPYTTLAVKTAQCMGGLSEANLATAWQYIDANM
                     NLGWDGEIIPNPMNEPIADDNIATVLVGNEALGEVIRRTSAALAPTTEALSTDEVIQM
                     LACDIADGQLDGVGPDVSPRVALTLLGTATAVRVEVISGALFVDGQDATPLLDSALQT
                     VLPGSLRSVTELAPNQQLIDQTLSGLAVLGTFDFAEATTLAIALEGATPDTARGQLAP
                     ALTSSVTTNLQDLGSQLALADTTTIEELLLRQADSAGAPVPVVSFDAEPSSVEAGTST
                     RLSWAASDADECLASGGWSGSQATSGTFQTPLLEQSTEYVLSCVGLGGTGTGRVFVEV
                     TQPAPVPATDAGSDPDPASDPDPVSDPISDPDPISDPDPISDPDPVSDPEPEPEPEPA
                     PAPAPPPPPPVVSISADRTTVDAGESALLTWNATDSESCSASGNWSGSRSVSGSLSIG
                     PLSASGTYALTCTGPGGSDTDSVSITVIEPAPRPGVVLTAASTSVEQGSATQLDWTST
                     DATSCSASGAWSGDRAISGSASTGVLSDDSTFTLTCSGPGGSASESVTVVVTEPPAPT
                     VNLTATSTEIDSGDSTTLTWSSSDATSCTASGSWSGDRGTSGDASVSPSATSSYTLTC
                     TGAGGATSASTTITVDPAPVPAPLVSLSVTPGTIDSGDTAVLSWTSSDTTSCTASGGW
                     EGTRDASGSVDVAPTATTTYTLTCSGDGGSDTATVTLSVVSAAPTVTFSASETLVDPG
                     GLVTVSWSSTDATGCTASGGWSGARDTSGSEVVGPVDSATTYTLTCTGPGGNAVEMLT
                     VETLNAVTLEWAAPAENVDGTPLTDLAGYRIYWGQTSRNYTDVVELNDPYTTSHTLNL
                     ASGDYHVAMTALDQDGNESGYSNEVVKTAQ"
     gene            complement(4171..>4822)
                     /locus_tag="AC16UZ_02090"
     CDS             complement(4171..>4822)
                     /locus_tag="AC16UZ_02090"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6245499.1"
                     /translation="ELKRCLKLGFKGMFMAPEPVCGRRPSHPDFDPIWQLLIDADLPI
                     CIHLIVRFDRVVNLSNTQWWDVTRERPDTVFSFGLGGTMQLIPAVAALVCDGLFERHP
                     RLKVAIVESGAGYVRYLMDRLDEKYARFKATSCLSRPPSEYIRENFWFVMDPSEASID
                     AQCDLVGEDKFLWGSDYPHVDSHLTAMQELHAALEPMSEHRRTLVLGENARRLFRI"
BASE COUNT          920 a         1603 c         1505 g          794 t
ORIGIN      
        1 gccacccact atctttcggc cggcatgaac gtgctgatcg ccgatctcga cgaggacgct
       61 ctgagcacag cggcagcgtc cctggctgac agtggcagcg gcaccctgca cactcaggtc
      121 tgcgacgtct ccgactttgc gcaggtggag acgctgcagg ccacagccgc ggcaaagctc
      181 ggcaacgtgc actgcctgat gaacaacgca ggtgcggggt tcccgcccag cgcgccctgg
      241 gagaacctgg acgtctggaa gaagcaggtg gagatcaacc tctggggcat cattcacggc
      301 tgccaggcct tcgtccctgc catgctggaa gccggcgagc ggggagcgat cgtcaatacc
      361 ggctcgaagc agggcatcac caacccgccg ggcggctacg cctacaacct gtccaaggcg
      421 ggcgtgaaga actacaccga gagcctggcc cacgcgctcc ggcaggtgga aggctgccag
      481 ctctccgccc acctgctggt gcccggcttc acctacaccg gcatgatctc ccgcttcatt
      541 cccgagaagc cggccggcgc ctggacgccc gacgaggtgg tggcatttct gattcgatcg
      601 ctcgatcgag gtgacttcta cgtgctctgc ccggacaacg acacgccgcg ggacctcgac
      661 gagaagcgca tccagtggaa cacggacgac ctgatcagga accgatccgc cctgtcccgc
      721 tgggatccga agtacaagga cgcctatgag gccttcgtct ccgactgaca atgcctggag
      781 ttccgtggct ggcggttgag cgcacctcgc ggcgccgctc cgatcgccta ccagggccag
      841 ctcatcgtca gctccggcta cggatttacc ggcaagatgt cgggcaacgc gctgctcgtt
      901 ttcgaggtgg caacttacgg ggcgggcgga acccaagaac ctcaaccgtt gtgcacccgt
      961 cacgttttct caaaccaacc acgatccttc cacaaaacgc tcgcatcagg gtcgaaagtg
     1021 tcgaccgcct cacgggaaga cacgtcccct tttccgatac tcgagccgtt ctgacgctat
     1081 gcgccgcaga tggcacccac cgatccgccg cgcagatgtt ggctttaaga gcggagaggc
     1141 aaagtgggac gaaacggaga gggaagggcc cccaggggaa cgcgggggtt tcaggcaggc
     1201 tgcctgagtg cgctactttt tctgggcgca tgcaccggca gcagcgaaaa gattttcgag
     1261 caaagtgaag agctgagtct tcgcggcagc gttggggacg gccccatcgt cggcgcgcag
     1321 atcgtggtgg aggacgccga aggccaaatg gtgttcaccg ccgagagcga tgccgccgcc
     1381 aactatcaga tcgatgttcc cgacggcacc gatctgcctg tgaccattcg ggtgagcggc
     1441 ggcaccgatc tggtcaccgg ccgcggcgcg gatttcgaac tgctggctgt ggcgttcacg
     1501 tctggcgatc tcaccgtcaa cgcctcaccc tacacgaccc tggcagtgaa gaccgcccag
     1561 tgcatgggtg gactcagcga ggccaatctc gctaccgcct ggcaatacat cgatgccaac
     1621 atgaacctcg gctgggatgg ggagatcatc ccgaatccca tgaacgagcc gattgccgat
     1681 gacaacatcg ccaccgtgct ggtgggtaac gaagccctcg gtgaggtgat ccgccgcacg
     1741 agcgccgcgc tggcgccgac cacggaagcg ctctccacgg acgaagtgat ccagatgctt
     1801 gcctgcgaca ttgctgacgg ccagctggac ggcgtcggac cagatgtgtc tccccgggtc
     1861 gcgctcacgc tgctcggcac ggcaaccgcg gtcagggtgg aagtcatcag cggtgccctg
     1921 ttcgtcgatg gacaggacgc caccccgctc ctggacagcg cgctgcagac cgtgctgccg
     1981 ggcagtctcc gctccgtcac tgagctggcg cccaatcagc agctcatcga ccagaccctg
     2041 agcggcctgg cggtgcttgg gacgttcgac tttgccgaag ccacgactct tgcgatcgcc
     2101 ctcgaaggtg caaccccgga taccgcccgc ggccagctgg cgccggctct caccagctcc
     2161 gtgaccacca atctccagga cctgggcagt cagttggctc tggcggatac gacaaccata
     2221 gaggagctgt tgctgcgcca agccgacagc gccggtgcgc cggttccagt cgtttcgttt
     2281 gatgcggagc catcgtctgt cgaggcggga accagcaccc gcctgagctg ggcggccagc
     2341 gacgccgacg agtgcctggc ctcgggtgga tggtcaggct cgcaggccac cagtggcact
     2401 tttcagaccc cgctgctgga gcagagcacg gaatatgtcc tgagctgcgt gggcctgggg
     2461 ggcacgggca ccgggcgagt gtttgtagaa gtgactcaac ccgcaccggt cccggcaacg
     2521 gacgcgggct ccgacccgga cccggcttca gacccggacc cggtctccga tccgatctca
     2581 gatccggacc cgatctcgga ccctgatccg atctcggacc cggacccggt ctcagatccg
     2641 gaaccggaac cggaaccgga acccgcaccg gctccggcgc ctcctcctcc gccgcccgtc
     2701 gtctccatca gcgccgatcg tacgacggtc gatgcaggcg agagcgcgct gctgacctgg
     2761 aacgcaacag actcagagag ctgcagcgcc tccgggaact ggtcaggatc ccgctccgtc
     2821 agcggcagtc tcagcattgg gcccctgtcc gccagcggaa cctatgctct gacctgtacc
     2881 ggtcccggcg gatcggacac cgacagcgtt tcgatcacgg tcatcgagcc tgctccccga
     2941 cccggggtgg ttctgacagc cgcatccacc agcgttgagc agggatcggc gacccagctc
     3001 gactggacca gcacggatgc aacgagctgc agcgccagcg gcgcctggag cggagatcgg
     3061 gctatctccg gctccgcttc taccggcgtg ctgagcgatg acagcacgtt taccctgacc
     3121 tgctcaggac ccggcggtag cgcttcggaa agcgtgacag tcgttgttac cgaaccacct
     3181 gctcccactg tgaacctgac cgcgaccagt acggaaatcg acagcggtga cagcacgact
     3241 ctgacctggt cgagcagcga cgccaccagc tgtaccgcca gcggtagctg gagcggcgat
     3301 cgcggcacca gcggcgatgc cagcgtgtcg ccgagcgcaa cgagcagcta cacgctgacc
     3361 tgcaccggag cgggtggggc aaccagtgcc agcacgacca tcaccgtcga cccggcccca
     3421 gtacctgcgc cgctggtttc cctgtccgtt actcccggca ccatcgacag cggcgacacg
     3481 gccgttctga gctggaccag cagcgacacc acaagctgca ccgccagtgg tggctgggaa
     3541 ggcacccgcg atgccagcgg cagcgtcgat gtggcgccca cggccaccac gacctacacc
     3601 cttacctgca gcggtgatgg cggcagcgat actgccaccg tgaccctgtc ggtggtgagc
     3661 gcggcgccga cggtcacgtt cagcgcctca gaaacactgg tcgatccagg cggcctcgtc
     3721 acagtcagct ggtcatcgac ggatgccacg ggctgtaccg cttccggcgg ctggagcggt
     3781 gcccgcgaca ccagtggctc cgaggtggtt gggccagtcg actcggcaac cacatacacc
     3841 ctcacctgca cgggcccagg aggcaatgcg gtggagatgc tcactgttga gacgctcaat
     3901 gcggtcactc tggaatgggc agcgcccgct gagaacgtcg acggtacccc attgaccgat
     3961 ctggccgggt atcggatcta ctgggggcag accagtcgca actacacgga cgtggtcgag
     4021 ctcaatgatc cgtataccac cagccatacc ctgaatctgg cctccggcga ttaccacgtc
     4081 gccatgacgg cgctggacca ggacggcaac gaaagcggct actcgaacga ggtggtgaag
     4141 accgcccagt agcgacttcg ctgtctctgg tcagatacgg aacagcctgc gcgcgttttc
     4201 accgagcacc agggtgcggc gatgctcgga catgggttcg agggcggcat ggagctcctg
     4261 catggcggtg aggtgggaat ccacatgggg atagtcagac ccccaaagga acttgtcctc
     4321 ccctaccaga tcgcactgag cgtcgatgct ggcttcgctc gggtccatga cgaaccagaa
     4381 gttttcccgg atgtattcgc tgggtggcct ggacaggcaa ctcgttgcct tgaacctagc
     4441 gtatttctcg tccaatcggt ccatcaagta gcggacgtat ccggcgccgg actcgacgat
     4501 ggccaccttc aaccgggggt ggcgctcgaa caagccgtcg cacacgagcg cggcgaccgc
     4561 cggtatcagc tgcatggttc cgcccagccc gaacgagaac accgtatccg gacgttcccg
     4621 ggttacgtcc caccactggg tattcgaaag attcaccacc ctgtcgaaac gtacgatgag
     4681 gtgaatgcat atgggcaggt cggcatcgat cagcaactgc catatgggat cgaaatccgg
     4741 gtgggaaggg cgcctgccac acacgggctc tggcgccata aacatgccct tgaagccgag
     4801 cttcaggcag cgcttgagtt cc
//
LOCUS       DBNHOX010000094         5488 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_778834, whole genome shotgun sequence.
ACCESSION   DBNHOX010000094 DBNHOX010000000
VERSION     DBNHOX010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_778834"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="AC16UZ_02095"
     CDS             <1..647
                     /locus_tag="AC16UZ_02095"
                     /inference="COORDINATES: protein motif:HMM:NF046326.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase-like TK C-terminal-containing
                     protein"
                     /protein_id="HIR6245500.1"
                     /translation="GLKRMVENGENVFFYITVMNESYRQPAMPEGVEEGILRGIYRVR
                     TVGDGETRRVRLLGSGTILREVEAAADMLHEDYGVSAEVFSVTSYNELARDGQDVERW
                     NLLHPEEPPRVSYVSQQLAGDDPVIASSDYMKSLAEQVRPYIGAPYKVLGTDGFGRSD
                     TRESLRHFFEVDRKFVALAALRSLADAQKIPLEEVRQARDAMGIDADKPNPRTA"
     gene            660..2342
                     /locus_tag="AC16UZ_02100"
     CDS             660..2342
                     /locus_tag="AC16UZ_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HIR6245501.1"
                     /translation="MATEIRIPDIGDVEHVEVVEICVAAGAEVAADDALIIIESDKAS
                     MEVPAGVAGVVASLAVAVGDTVAEGDLIAVIEAAGAAPVEDQAGEVAAEAPPQMQDPK
                     VPPERAVASPPTKPEAGPSGTVEIRVPDIGEAEDVVVVEVAAAAGQQVGEDDLLVVVE
                     SDKASMEIPAGCSGTVLEMHVSEGAAVTEGTLIATVEVKGSSAAPAPAADRPEVEAGE
                     VPRGAEAAAPQPISTTAAPPAGPDTAAAEQESAGTALGERIYAGPAVRRLARELGVDL
                     ARVRGTGARERIVKDDVKAYVKDAMTAAPGGAGAGAASGAGIPAIPQIDFSRFGPVEI
                     RPLTRIRAAGAANLQRSWLNLPHVTQHDEADVTELEAFRASLKSEAERRGVKITPLAF
                     IIKACCYALKKFPTFNASLDAEARNYVLKGYYNIGFAVDTEEGLVVPVIKGADQMGIW
                     ELSEAVTDLAGRAREQKLGMDDMQGGSFSVSSLGAIGGTGFTPIINAPEVGILGVSRL
                     AMRPVWDGESFVPRKLLPLSLSYDHRAINGAEAGRFVSHLCELLADIRRLSL"
     gene            complement(2361..2894)
                     /locus_tag="AC16UZ_02105"
     CDS             complement(2361..2894)
                     /locus_tag="AC16UZ_02105"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR6245502.1"
                     /translation="MTTKAGDTGRTSLADGSKLAKTEPRMHAIGTVDELNSFIGLLVT
                     QLDPDAALREPCARIQQELFDIGATLATVGAIPCPDNAWIDEEVQTLNARLPPLTEFV
                     IPGGTVAAALAHVCRTTCRRAERELWALQDDDSATAAARYTNRLSDLLFVMARTLNAA
                     EGAEPQWRGTRGPKGPE"
     gene            complement(2939..3928)
                     /locus_tag="AC16UZ_02110"
     CDS             complement(2939..3928)
                     /locus_tag="AC16UZ_02110"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HIR6245503.1"
                     /translation="MSWEDIPILVVDDAKYSSAVITKALTDEGFSNVRCVNAGFAALD
                     TLENRSAQILIADWVMPAMDGLELTRRVKRLDEENGHFTYVMLLTGREDFEGMLEAFD
                     TGVDDFLNKHNLRAELQPRIIAAQRIAARQNELLRTNKLLRKKVRELSVVDLIDPVTG
                     LGNRRFTIERLTAAMEQAESRRMAACLLLVGVDNVAALTERYGPPAVDELMSGIGSKI
                     RTLVRPLDLVTRPEPGIFAVITLHPSIDECTSSTFQRIFDGLYLNSFKTSEAYIPVSV
                     GISITAADAQTGFPHPRQLMRQAVHALGESFESGQIEVQRAATLNEPVTAAEL"
     gene            4137..4619
                     /locus_tag="AC16UZ_02115"
     CDS             4137..4619
                     /locus_tag="AC16UZ_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HIR6245504.1"
                     /translation="MPSFDVISEVDLQEVRNAVDQASRELRTRFDFKGVDASFEYTDE
                     GILLAAMEEFQLGQMLDILKDKLNRRGVDVRALDPGTIEASGKQKRQAFALKQGIDRD
                     TAKRLVKILKDSRLKVQSQIQGEQVRVTGKKRDDLQQAIAALKDAGLEVPLDFTNFRD
                     "
     gene            4645..>5488
                     /locus_tag="AC16UZ_02120"
     CDS             4645..>5488
                     /locus_tag="AC16UZ_02120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6245505.1"
                     /translation="MKLREVLNRRMITCAFTGFASGLPLFVLVQLVQAWLRDEGVGLT
                     EIGLFALAQVPYTWKFIWSPLAERYPLPFLGRRRGWMLVTQVALVAGFAGFGYVDPVL
                     NIEAVFALALLVGFFSASQDIVIDAYRRELLPTEEELALGNSIHVQVYRIAGFVPGSL
                     GLILADVLPWSAVYWTMAGFMAIGVATTLVVREAVLDPRIPPSLKAAVVEPFREYLGR
                     RGWRYGLVVLLFLFAYKFGDSMATALSTTFYLDLGFSKTEIGVVAKNAGFWPALIGGL
                     LGGLF"
BASE COUNT          898 a         1685 c         1924 g          981 t
ORIGIN      
        1 atggtctgaa gcggatggtc gagaacggcg agaacgtttt cttctacatc acggtgatga
       61 acgagagcta tcggcagccg gccatgcccg aaggggtgga ggagggcatc ctgcggggca
      121 tctaccgggt gcgcacggtt ggcgatggcg aaacgcggcg ggtacgcctg ctcggcagcg
      181 gaaccatact gcgcgaggtc gaagcggctg ccgatatgct gcatgaggac tacggcgtca
      241 gcgccgaggt gttcagcgtc acgagctaca acgagctggc tcgcgacggc caggacgtgg
      301 agcgttggaa cctgctgcat ccggaagagc cgccgcgggt gagctacgtc tcgcagcagc
      361 tcgccggaga cgatccggtg atcgcctcca gcgattacat gaagtccctg gccgagcagg
      421 tccggcccta catcggggcg ccgtacaagg tgctcggcac ggatgggttc ggacgcagcg
      481 ataccaggga atcgctcaga cacttcttcg aggtcgaccg caagttcgtc gcgctggccg
      541 cgctgcgcag cctggcggat gcccagaaga tcccgctcga agaggttcgc caggccaggg
      601 atgccatggg catcgatgcc gacaaaccca atccgcggac agcctgaggg agcctggagt
      661 tggccacaga gatacgcatt cccgacatcg gcgatgtgga gcacgtggag gtggtggaga
      721 tctgcgtcgc agcgggtgcc gaggtggccg cggacgacgc gctcatcatc atcgagtccg
      781 acaaggcgtc catggaggtg ccggcagggg tggcgggcgt ggtggcttcc ctggcggtgg
      841 ccgtgggcga caccgtggcc gagggcgatc tcattgccgt catagaggct gccggggcgg
      901 cgccggtcga ggatcaggcc ggcgaggtgg ccgctgaggc gcccccgcaa atgcaggatc
      961 cgaaagtgcc cccggaacgg gccgtcgcct cgccgccgac aaagcccgag gctggcccgt
     1021 ccggtaccgt cgagatccgg gtgccggaca tcggcgaggc cgaggacgtc gtggtggtgg
     1081 aggttgcagc cgccgccggg cagcaggttg gcgaggacga tcttctggtc gttgtggagt
     1141 ccgacaaggc gtccatggag ataccggcag ggtgttccgg aacggttctg gaaatgcacg
     1201 tatcggaagg tgcggcagtt accgaaggga ccctcatcgc cacggtcgag gttaagggat
     1261 cgtcggcggc ccccgcacct gcggcagatc ggcccgaggt cgaagcaggc gaggttccca
     1321 ggggcgccga agcagcggcc ccgcagccta tctcaacgac ggctgcgccc ccggccggcc
     1381 cggacactgc ggcggcggag caagagtcgg ccgggacggc tctgggcgaa cgcatctacg
     1441 ccggcccagc cgtgcgccgg ctcgcccgtg agctgggggt ggatctcgcc cgggtgaggg
     1501 gcaccggcgc gcgggagcgc atcgtgaagg atgacgtcaa ggcgtacgtc aaggatgcca
     1561 tgaccgcggc gccgggcggt gccggagcag gggccgcgag cggagcgggc atcccggcca
     1621 taccgcagat cgacttttcc agattcggcc cggtggagat ccggccgctg acgcggattc
     1681 gcgcggcggg cgccgccaac ctgcagcgca gctggttgaa cctgccccac gtcacccagc
     1741 acgacgaggc ggacgtcacg gagctcgagg cctttcgcgc ctctctgaag agcgaggcgg
     1801 agcggcgggg cgtgaagatc acgccgctgg cattcatcat caaggcctgc tgctacgcgc
     1861 tgaagaagtt cccgaccttc aacgcctccc tggacgctga agcccgcaac tacgttctaa
     1921 agggttatta caacatcggc ttcgccgtcg acaccgagga gggcctggtc gttccggtta
     1981 tcaaaggcgc ggatcagatg ggcatctggg agctctccga ggctgttacc gacctcgcgg
     2041 gcagggcccg cgagcagaag ctgggcatgg acgacatgca gggcgggagc ttcagcgttt
     2101 cgagcctggg ggccattggt ggaaccggct tcacgcccat catcaatgcc ccggaggtgg
     2161 gcatcctggg tgtgtcccgg ctcgccatgc ggccggtctg ggacggcgag agcttcgtgc
     2221 cgagaaaatt gctcccgctg tcgttgagct acgaccatcg ggcaatcaac ggcgccgagg
     2281 cgggcaggtt cgtcagccat ctctgcgagc tgctggccga catccgccgg ctttctctct
     2341 aggcctttcg ggcccctgcg ctactccggg cccttcgggc cgcgcgtgcc gcgccactga
     2401 ggctcggctc cctcggcggc gttcagggtg cgggccatca cgaacagcag atcgctcagc
     2461 cggttggtat agcgggcggc cgctgtcgcc gaatcgtcgt cctgcaaggc ccacagctcg
     2521 cgctcggcgc ggcggcacgt cgtcctgcag acgtgggcca gagcggccgc gaccgtgccg
     2581 ccggggatga cgaactcggt aaggggcggc aggcgggcat tcagcgtctg aacctcctcg
     2641 tcgatccagg cattgtcggg acaggggatc gctcccacgg ttgccagcgt ggcgccgatg
     2701 tcgaagagct cctgctggat tctggcgcac ggctcgcgca gcgcagcgtc ggggtcgagc
     2761 tgcgtgacga gcagcccgat gaagctgttc agctcgtcga cagtgccgat cgcgtgcatg
     2821 cggggttctg tcttggcgag cttagagccg tccgccagcg acgttctgcc ggtgtcgccg
     2881 gccttcgtgg tcacctgggt tatccgcttg cgctctcgtg tcatgggtgc gtggcggctc
     2941 agagctctgc ggcggtcacg ggctcgttca gggtcgccgc ccgctggacc tcgatctggc
     3001 cggactcgaa cgattcgccc agggcgtgca ccgcctggcg catgagctgg cgcggatggg
     3061 gaaagccggt ctgggcgtcg gcggcggtaa tcgatatccc gacggagacg ggaatgtagg
     3121 cctcgctggt cttgaaagag ttcagataga gcccgtcgaa gatgcgctga aaggtgctgg
     3181 acgtgcactc atcgatgctc gggtgcagcg tgatcaccgc gaagatgccg ggctcgggcc
     3241 tggtgaccag gtccaggggc cgcacgaggg tgcggatctt cgacccgatg ccgctcatca
     3301 gctcgtccac cgcgggcgga ccgtaccttt ccgtgagcgc cgcgacgttg tcgacgccca
     3361 cgagtagaag gcatgccgcc attcggcggc tttccgcctg ctccatggcg gctgtgaggc
     3421 gctcgatggt gaagcggcgg ttgcccaggc cggtcaccgg gtcgatgaga tcgaccacgc
     3481 tgagctcgcg taccttcttt cgcaggagct tgttggtgcg cagcagctcg ttctggcgcg
     3541 cggcgatgcg ctgggcggct atgattctgg gctgcagctc ggcgcgcagg ttgtgcttgt
     3601 tgagaaagtc gtccaccccg gtgtcgaaag cttccagcat gccttcgaaa tcttccctcc
     3661 ccgtgagcag catcacgtac gtgaagtggc cgttctcctc gtccaggcgt ttcacccggc
     3721 gggtcagctc cagcccgtcc atggcaggca tcacccagtc ggcgatgagg atctgggccg
     3781 aacggttttc cagcgtgtcc aacgcggcga agccggcgtt cacgcagcgc acgttgctga
     3841 acccttcgtc ggtcaaagct ttggtgatga cggcgctgga atacttcgcg tcatcgacga
     3901 cgaggattgg aatgtcttcc catgacatgt aagttagcgt ttgcttcgac gccttgtcgc
     3961 ttccttgata tgcgagggac ccctcgatgt caaggaaggt tcatcgctcg tgcggccatg
     4021 gtctcaggat ggtgatctgg ttcacctttc tgcgccgcgt ggggccggcg cctgcagcgc
     4081 gaaaccaggc gcctggggcg cgatgctaga tgctactaga tgccaaagga aacgctatgc
     4141 caagcttcga cgtgatttcc gaggtggatc tgcaggaggt tcgcaacgcg gtggatcagg
     4201 catcgcggga gcttcgcacc cgattcgatt tcaaaggcgt cgacgccagc ttcgagtata
     4261 cggacgaggg catccttctg gctgccatgg aggagttcca gctgggtcag atgctggaca
     4321 tcctcaagga caagctgaac cgccgcggcg tggacgtccg tgctctcgat cccggcacga
     4381 tcgaggcgtc cggcaagcag aagcggcagg ccttcgctct caagcagggt atcgaccgcg
     4441 atacggccaa gcgcctggtc aagatcctga aagactccag gctaaaggtc cagagccaga
     4501 ttcagggcga gcaggtgcgc gtgacgggga agaaacggga cgatcttcag caggccatcg
     4561 ccgccctgaa ggacgcaggc ctggaggtgc cgctcgactt caccaacttc cgcgactgac
     4621 gcagcccctg ccggacggcc cgccttgaaa ctccgtgagg tactcaaccg gcgcatgatc
     4681 acatgcgcgt tcacgggttt cgcatcgggc ctgccgctgt tcgttctggt gcagctggtg
     4741 caggcgtggc tgcgcgacga gggcgtcggg ctgacggaga tcgggctgtt cgcgctcgct
     4801 caggttccgt acacgtggaa gttcatctgg tcgccgctgg cggaacgcta cccgctgccc
     4861 ttcctggggc gccggcgggg ctggatgctg gttacccagg tcgcgctggt tgccggcttt
     4921 gcggggttcg gctacgtgga tcccgtgctg aacatcgaag ccgtgttcgc cctggcgctg
     4981 ctggtcggtt ttttcagcgc cagtcaggac atcgtcatcg acgcctatag gcgcgagctg
     5041 ctgcctaccg aagaagagct tgcgctcggc aactccattc acgttcaggt ataccggatt
     5101 gccggtttcg tgccgggctc cctcggcctg atcctcgccg acgtgctgcc ctggagcgcc
     5161 gtgtactgga ccatggccgg cttcatggcc atcggcgtgg cgaccacttt agtggtgcgc
     5221 gaggccgtgc tggacccgcg gataccgcca tcgctgaaag ccgccgtggt ggagcctttt
     5281 cgggaatatc tcggccgccg cggatggcgc tacgggctgg tggttctgct gttcctgttt
     5341 gcctacaagt tcggcgacag catggcgacc gccctgtcga cgacctttta cctcgatctt
     5401 ggattcagca agacggaaat cggcgtggtg gccaagaacg ccggattctg gcccgcgctc
     5461 atcggcggcc tgctgggcgg tctgttca
//
LOCUS       DBNHOX010000095         4017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_522286, whole genome shotgun sequence.
ACCESSION   DBNHOX010000095 DBNHOX010000000
VERSION     DBNHOX010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_522286"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="AC16UZ_02125"
     CDS             complement(<1..720)
                     /locus_tag="AC16UZ_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245506.1"
                     /translation="MTGLFLRALLAWLAVCPALVEGAEAVSPETATAGEPGRPLPMES
                     DGKRNPLTPDVRDDDTELTMQRGDFVIAPIPISDPVLGEGLVLAAAYFYPQTEEQAAS
                     QPASVTAGAGMYTSSESYAYGIGQQNYWGEDRWRFSGALAHADLQLKLRPGAENGDGG
                     RTDWLIDGNVLQAELSRMIAGDWYLGLISRFVDINQSFETDRQTGNFDLGSSIRTVGM
                     GLSVEHDSRDLPLNSFSGRLFE"
     gene            952..2289
                     /locus_tag="AC16UZ_02130"
     CDS             952..2289
                     /locus_tag="AC16UZ_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245507.1"
                     /translation="MNIRNVSLALILAAVCVPSQATDTEEQQEFDSPLILKASEVLSA
                     ETRNGEHHEVREEIVNDGFMNTYVIDSPFGTFEAYGSSMLRTRINEIGALAELDGLSK
                     TKVFMEAAAQSAVRAVTAPVSAAATIVTNPIGTVTGLPGGLARMFNRQKRKVEGAVAT
                     TASAAKSATGADDSGEEASIAEQAGEGAVDLGKSLMGTTRAERRWAKKLGTDPYTSNE
                     TLRKGIADVAVVDSSGYFLAELAVPIGGIVDLVVDVNEAIWERDAYELRQMNLVKLQD
                     MGVAEEEATAFLDNVAISPTIQTLIINGLGLLETTAGRDAFLRQATLAEDEAESRFYL
                     QSLAMIAWLNQEGENVAAITVHGPVSIAVDTGGNRVAMLPVDHLTWNPTTVEAVGLWP
                     AQDPDVPSDGRVYISGDISALARSHLEQAGWSARSKIARDFFTYLSSQESDEP"
     gene            complement(2395..3933)
                     /locus_tag="AC16UZ_02135"
     CDS             complement(2395..3933)
                     /locus_tag="AC16UZ_02135"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008031994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR6245508.1"
                     /translation="MRRILLAMGASLLLPAMGLAADKPNILVIWGDDIGQSNISAYTR
                     GMMGYPTPNIDRIAAEGILFTDYYGEQSCTAGRSSFIMGQSVFRTGLSKVGLPGAEEG
                     MQVEDPSIAGLLKDQGYATGQFGKNHLGDRDEHLPTNHGFDEFFGNLYHLNAEEEPEN
                     EDYPKNPEFRERFGPRGVIRSSADGQIEDTGPLTKKRMETVDDETVAAAMDFMERQHA
                     AGKPFFVWWSGTRMHFRTHVKDEMRGISGQDEYSDGMVEHDMHVGMFLDKLDALGIAD
                     NTIIHYSTDNGPHYNTWPDAASTPFRGEKNTNWEGGWRVPAAVRWPGVVKPGTVSNGI
                     VHHMDWLPTFLAAAGKTDIKEDLLDGYRSPAMKRRYRVHLDGYDLTDHLKDPQGVESP
                     RKEIFYFSDDGDLTALRYQDWKLIFMEQRVEGTLQAWAEPFVPLRVPLLFNLRRDPYE
                     RSQKTSNTYYDWLIDRVFLMVPAQVYVGQFLATFREYPPRQKAASFSLERVLEQLQEG
                     GDSR"
BASE COUNT          758 a         1292 c         1249 g          718 t
ORIGIN      
        1 ctcgaagagc cggcccgaga aggagttcag cgggaggtcc cggctgtcgt gttccacgct
       61 gaggcccatc ccgacagtgc gaatactcga gcccaggtca aagtttccgg tttgccggtc
      121 tgtttcaaag ctctggttga tatccacgaa ccggctgatc aggcccaggt accagtctcc
      181 cgcgatcatg cgtgaaagct ctgcctgcag gacgttgcca tcgatcagcc aatcggtccg
      241 ccccccgtcg ccgttctccg cgccggggcg aagcttcagc tgcaagtcgg catgagccag
      301 ggcgccgctg aagcgccatc tgtcctcgcc ccagtagttc tgctggccga taccgtaagc
      361 gtagctctcg ctgctggtat acattcccgc ccccgcggtg acggacgcag gttgggaagc
      421 tgcctgctcc tccgtctggg gatagaaata ggcggccgcg agaaccagac cctctccaag
      481 caccgggtcc gaaatcggaa tgggggcaat cacgaagtcg ccccgctgca tcgtcagctc
      541 ggtatcgtcg tcgcgaacat cgggcgttag agggttcctc tttccatccg attccatcgg
      601 cagtggccgg ccgggctcgc ccgccgtcgc cgtttccggg gagaccgctt cggccccctc
      661 caccagcgca ggacacacag cgagccaagc gagcagcgcg cgcaggaata agcccgtcat
      721 ccaatcgtca cctcgggcag caacgtccgc caccctgccg gcccagtcgt cgcctgacac
      781 tgaccataag ctttcccgga agagccctcg cgatggcgcg agccacatga tttccctggc
      841 ggatgcagcc ctcgatccac ccgactaaga tgtgaagacc ccgcagcgat gttcccggcg
      901 agggacctgc cggcatcaga gccggttctc gacaacgccc aaggattttc tatgaatata
      961 cgtaacgtct cgctggcgct cattctagcc gcggtgtgcg tgccatccca agccaccgac
     1021 accgaggagc agcaggaatt cgattccccc ttgattctga aggccagcga agtgctcagc
     1081 gccgagaccc gcaacggaga gcaccacgag gtacgcgagg aaatcgtcaa cgatggcttc
     1141 atgaacacct acgtcatcga ctcgcctttc gggacatttg aagcctacgg ctcgtccatg
     1201 cttcgaacgc gcatcaacga gataggcgcg ctggccgagc tggacggcct gtcgaaaacc
     1261 aaagttttca tggaagcagc tgcccagtcg gcggtaaggg ccgtcaccgc ccccgtcagc
     1321 gccgccgcga ccatcgtcac gaaccccatc ggcaccgtca ccgggctccc cgggggcctg
     1381 gcacgcatgt tcaacaggca gaaacgcaaa gtcgagggcg cggtggccac gacggcgtcg
     1441 gcagcaaagt cggcgacggg cgccgacgac tccggggagg aggccagcat cgcggagcag
     1501 gcaggcgagg gggccgtcga tctcggcaaa agcctgatgg gaaccacccg cgcggagcgg
     1561 cgctgggcca agaagctggg taccgatcca tatacctcca acgaaactct cagaaaggga
     1621 atcgccgacg tggccgtcgt cgacagcagc ggctatttcc tggccgagct ggcggtgccc
     1681 atcggaggca tcgtcgatct ggtggtggac gtcaacgagg ccatctggga acgcgacgcc
     1741 tacgagttgc gccagatgaa cctggtcaag ctgcaggaca tgggtgtggc ggaggaggag
     1801 gcgaccgcgt ttttagataa cgtcgccatc agccccacca ttcagaccct gatcatcaac
     1861 ggtctggggc tgctcgaaac cactgccggg cgggacgcct tcctgcgtca ggcgaccctg
     1921 gccgaggacg aggccgaatc ccgattctac ctccagagcc tggccatgat cgcgtggttg
     1981 aaccaggagg gggagaacgt tgctgccatc accgtgcacg gacccgtctc gatcgccgtc
     2041 gacaccggcg gcaaccgtgt cgccatgctt cccgttgacc acctgacgtg gaacccgacc
     2101 accgtcgagg ccgtcggcct gtggccggca caggaccccg acgtcccttc ggacggccgg
     2161 gtctacattt ccggagacat ctctgccctg gcgagaagcc atctggagca agcaggctgg
     2221 tcggcacgat ccaagattgc ccgggatttc ttcacgtatt tgagcagcca ggagtcagac
     2281 gagccgtagc ccgccggttt cgaacgggag agcgtctgcg gaaaggacct ggcgcttcag
     2341 gagaagcagc gggcgaccgc cgaatacggc cgcccactgg gtcgatgaac ggcgctagcg
     2401 cgagtcgccg ccctcctgca gctgctcgag gacccgctcc agggagaagc tcgccgcctt
     2461 ctggcgcggc gggtactccc tgaacgtggc caggaactgg cccacgtaga cctgcgccgg
     2521 caccatgagg aagacccgat caatgagcca gtcgtagtag gtgttggacg tcttctgcga
     2581 gcgctcgtaa ggatcgcgcc tcaagttgaa gagcagcgga acacgcagcg gcacgaaggg
     2641 ctcggcccag gcctgaagcg tgccttcgac gcgttgctcc atgaagatca gcttccagtc
     2701 ctgatatcgt agagccgtca gatcgccatc gtcggagaag taaaaaatct ccttgcgcgg
     2761 gctttccacc ccctggggat ccttgaggtg gtcggtgagg tcgtagccat cgagatggac
     2821 acggtagcgc cgcttcatgg caggggagcg gtacccgtcc agaaggtctt ccttgatgtc
     2881 ggtctttccc gccgccgcca gaaatgtcgg cagccagtcc atgtgatgaa cgatgccgtt
     2941 acttaccgtc ccgggcttca cgacgccagg ccagcgcacg gctgccggca cgcgccagcc
     3001 gccttcccag ttggtgttct tctcgccccg gaaaggcgta gaggcagcgt ccggccaggt
     3061 gttgtagtgc gggccgttat ccgtggagta gtggatgatg gtgttgtcgg caatgccgag
     3121 cgcatccagc ttgtccagga acatccccac gtgcatgtcg tgctcgacca tgccgtccga
     3181 gtactcgtcc tgcccggaaa taccgcgcat ctcgtccttg acgtgggtgc ggaagtgcat
     3241 gcgggttccg ctccaccaga cgaagaacgg cttgcccgcg gcatgctgcc gctccatgaa
     3301 atccatggcc gccgccacgg tttcgtcatc gacggtttcc atgcgtttct tggtgagcgg
     3361 cccggtgtcc tcgatctggc cgtcggcgct cgagcgaatg acgccgcgcg ggccgaaacg
     3421 ctcgcgaaac tcgggattct tcgggtagtc ctcgttctcg ggttcctctt cggcgttcag
     3481 gtgatacaga ttgccgaaga actcgtcgaa accgtgattg gtgggcaggt gctcgtctcg
     3541 gtcacccaga tggttcttgc cgaactggcc cgtagcgtag ccctggtcct tcagcaggcc
     3601 ggctatggag gggtcctcca cctgcatgcc ttcctccgcg cccggcagac cgaccttcga
     3661 cagcccggta cggaagacgc tctgacccat gatgaaggac gagcgcccgg cggtgcagct
     3721 ctgctcgccg tagtagtcgg tgaacaggat accctcggcc gctatccggt cgatgttggg
     3781 cgtggggtag cccatcatgc cgcgggtgta ggcactgatg ttgctttgac cgatgtcgtc
     3841 gccccagatg acgagtatgt ttggcttgtc ggcagccagc cccatggcgg gcaagagcaa
     3901 agacgcgccc atcgcgagca aaatgcgtcg catgtgtaaa ctccctagcg tttcaaaaat
     3961 ggattttcgt tgagcccgcc catagcgagc ttcgaggcat ctgcagcgcg ccgcaca
//
LOCUS       DBNHOX010000096         3066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1550564, whole genome shotgun sequence.
ACCESSION   DBNHOX010000096 DBNHOX010000000
VERSION     DBNHOX010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1550564"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="AC16UZ_02140"
     CDS             complement(<1..505)
                     /locus_tag="AC16UZ_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6245509.1"
                     /translation="MADTAAHAFVGQRFGRWLDRRIPPGSRITLSQRNVFIFPTPTGF
                     VFGGLLILLVLGAINYQASLVYGVAFLLGSVFLMTILYTFRNLSGLSLELVSTRAGFV
                     GEAVEFSVRVVRPKGAGREGIQLGWPNGIKQWAELYEQEADTLRLYVKGERRGYLNPG
                     RLLVETYY"
     gene            complement(511..1485)
                     /locus_tag="AC16UZ_02145"
     CDS             complement(511..1485)
                     /locus_tag="AC16UZ_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6245510.1"
                     /translation="MRKSTPIRRSSSYTTTPEAKDLIQALLSALNTVLLGKETQVKLA
                     LGCLFARGHLLIEDLPGMGKTLLSHALAKVLGLSYNRIQFTSDVLPGDIIGTSIFERD
                     TGGFRFIEGPLFAQLVLADEINRATPKSQSALLEAMEERQVTIEGKTRSLPEPFFVIA
                     TQNPTTQTGTFPLPESQLDRFLMRIELGYPEPDAERELLRGGDRRAVLEGLEPVSDLE
                     GLRALQRAVDEVKVSDLLIEYVQRLVAHSRRGSEFAFGLSPRGALALLHGARAWALIN
                     DRSHVVPEDVQAVLPSVVEHRLREAADFSGHGGGALAQKLLSSVDVVG"
     gene            complement(1433..2959)
                     /gene="purF"
                     /locus_tag="AC16UZ_02150"
     CDS             complement(1433..2959)
                     /gene="purF"
                     /locus_tag="AC16UZ_02150"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR6245511.1"
                     /translation="MCGIVGMVGTQGINQQLYDALTVLQHRGQDAAGMVTSDGNRVHL
                     RKGNGLVRDVFHQQHMQRLVGNVGIGHCRYPTAGSASSAEAQPMYVNSPYGIALAHNG
                     NLTNSESLQADLFREDLRHVNTDSDSEVLLNVFAHELQVQRALQPEPEHIFAAIEGLH
                     RRCRGAYAAIAMIIGVGVVAFRDPCGIRPLVFGRRENGAGFEYMVASESVALDILGFE
                     RIRDVAPGEAVFITNEGDLHTRVCAEQTSLSPCIFEYVYLARPDSIMDEVSVYKCRLR
                     MGEYLAQRILERYAGNDHDIDVVVPIPDTGRTAALPLAYQLNVKYREGFIKNRYIGRT
                     FIMPGQSQRRKSVRQKLNAVDLELRGKNVLLVEDSIVRGTTMQHVIQMARDAGAKKVY
                     MASAAPPIRFPNVYGIDMPTPKEFVAYDRTVEEVREFIGADWLVYQELEDLVASAREG
                     NPTISQFECSVFDGHYVTGDVDAGYLERISLHRSDQMKQRRDAEINADQTVIELHNHA
                     "
BASE COUNT          580 a          988 c          950 g          548 t
ORIGIN      
        1 ggtagtaggt ctcgaccagc agccgcccgg ggttgagata tcctcgtcgc tcacccttca
       61 cgtacaaccg cagggtatcg gcctcctgct catagagctc ggcccactgc ttgatgccgt
      121 tcggccatcc cagctgaatc ccctcgcgcc cggcgccttt tggacgcacc acgcgaacgc
      181 tgaactccac cgcctcgccg acgaacccgg cgcgcgtgct gaccagctcc aggctgagcc
      241 ccgacagatt gcggaacgtg tagagaatgg tcatcaggaa gacgctgccg agcaggaagg
      301 cgacgccgta gaccaggctc gcctgatagt tgatggcgcc gaggacaagc aggatgagca
      361 gaccgccgaa aacgaacccc gtcggggtcg gaaagatgaa aacgtttcgc tgactgagcg
      421 tgatacgcga accaggcggg atgcgtctgt cgagccagcg cccgaaccgt tgcccaacga
      481 aggcgtgagc ggctgtgtcg gccacggatg ctagcctacg acgtcaacgc tcgacagcag
      541 cttctgcgcg agcgcgccgc ccccgtgacc cgagaagtcg gcagcttccc gcagccggtg
      601 ctccaccacg cttggcagca cggcctgcac gtcttccgga accacatggc tgcgatcgtt
      661 gatgagcgcc caggcccggg cgccgtgcag cagggccagg gcgccgcgag gcgaaagccc
      721 gaaggcgaac tccgatccac gacggctgtg cgccaccagc cgctgcacgt actcgatcag
      781 cagatccgag accttcacct cgtccaccgc tcgctgcagg gcgcgaagcc cttccaggtc
      841 cgaaaccggt tcgagcccct cgagcacggc gcgccggtct ccccctcgca gaagctccct
      901 ctcagcatcg ggttccgggt acccgagctc gatacgcatc aggaagcggt ccagctgaga
      961 ctcgggcagc gggaaggtgc ccgtctgagt ggtggggttc tgagtcgcga tcacgaagaa
     1021 cggttcgggc agcgaccggg tcttaccctc gatggtgacc tgccgctcct ccatggcctc
     1081 taggagcgcg ctctggctct tgggcgtcgc gcggttgatc tcgtctgcaa ggaccagctg
     1141 ggcaaagaga ggcccctcga tgaaacgaaa cccgccggtg tcccgttcga agatggacgt
     1201 tccgatgatg tcgcccggaa gcacgtcact ggtgaactga atgcggttgt agctcaaccc
     1261 gagcaccttc gcgagcgcgt gggagagcag cgtcttgccc atcccgggca ggtcctcgat
     1321 gagcagatga ccccgggcaa agaggcagcc gagcgccagc ttgacctggg tctctttgcc
     1381 cagaaggacg gtgttgagag cggacaggag ggcctggatc aggtccttgg cctcaggcgt
     1441 ggttgtgtag ctcgatgacc gtctgatcgg cgttgatttc cgcatcccgg cgctgtttca
     1501 tctggtcact tcgatgcaac gatattctct ccagatagcc ggcgtcgacg tcaccggtca
     1561 cataatggcc atcgaagacc gagcattcga actgggagat ggtcggattg ccctcccggg
     1621 cgctcgccac cagatcttcc agttcctggt aaacgagcca gtcggcgccg atgaactcgc
     1681 ggacctcctc gacggtccgg tcgtaggcga cgaactcctt aggcgtcggc atgtcgatgc
     1741 cgtaaacgtt tggaaaccgg atgggcggtg ccgcagatgc catgtagacc tttttcgccc
     1801 cggcatcccg cgccatctgg atgacgtgct gcatggtggt tccgcgaacg atggagtcct
     1861 ccaccagcag cacgtttttg ccacgcagct cgagatccac ggcgttcagc ttctggcgta
     1921 cggatttccg gcgctgactc tgacccggca tgatgaacgt ccgcccgatg tagcggttct
     1981 tgatgaaacc ctcccgatac ttgacgttaa gctggtaggc gagagggaga gcggccgtcc
     2041 gccccgtatc cggaatcggc acgaccacgt cgatgtcgtg atcgttcccg gcataccgct
     2101 ccagtatccg ctgggcaagg tactcgccca tcctcaggcg acacttgtac acggacacct
     2161 catccatgat ggagtccggt cgcgcgagat agacgtactc gaagatgcac ggagacagcg
     2221 acgtctgctc cgcgcagacc cgggtgtgaa gatcaccctc gttggtaatg aacaccgcct
     2281 cgccgggcgc gacgtcacgg atccgctcaa agccgagaat gtccagggcc acgctttccg
     2341 aggcgaccat gtactcgaac ccggccccgt tctcacgccg gccaaacacc agcggccgga
     2401 tgccgcaagg gtcgcgaaac gcaaccacgc cgacgccgat gatcatggcg atggcggcgt
     2461 aagcgccccg acagcgccgg tggagacctt cgatggctgc gaagatgtgc tcgggctccg
     2521 gctgaagggc tcgctggact tgcagctcgt gagcgaacac gttcaacaga acctctgaat
     2581 cgctgtccgt gttgacgtgg cgaaggtcct cgcggaacag atcggcctgc aggctttccg
     2641 agttggtcag gttcccgttg tgagcaaggg caatgccata aggcgagttg acgtacatgg
     2701 gctgcgcttc cgccgagctc gccgagccag ccgtggggta gcggcagtgt ccaatgccga
     2761 cgttgcccac cagacgctgc atgtgctgct ggtgaaagac gtcgcgaacg aggccgttac
     2821 ccttgcgcag atgcacgcgg ttgccatcgc tggtgaccat gcctgccgcg tcctgtccac
     2881 ggtgctgaag caccgtgagc gcatcgtaga gctgctggtt gatgccctgg gtgccgacca
     2941 ttccgactat tccgcacatg tctgctcagc gcccctcact aacatcttcg aatacgtcat
     3001 gagctctgcc catcagttcg agcacgtcct gctcgaactc gagcagcgcg ggcgagaaat
     3061 cggact
//
LOCUS       DBNHOX010000097         2806 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_200806, whole genome shotgun sequence.
ACCESSION   DBNHOX010000097 DBNHOX010000000
VERSION     DBNHOX010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_200806"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /gene="soxX"
                     /locus_tag="AC16UZ_02155"
     CDS             complement(<1..342)
                     /gene="soxX"
                     /locus_tag="AC16UZ_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HIR6245512.1"
                     /translation="MIARAALVLTTALLVVLAPDQAKAEAPDDLERGKAIASDRNAGN
                     CLSCHMMGDGEMPGNSGPPLLQMRLRFPDRAVLRAQIWDATERNPDSVMPPYGRNLIL
                     TEEEIDRVVDYV"
     gene            complement(339..1277)
                     /locus_tag="AC16UZ_02160"
     CDS             complement(339..1277)
                     /locus_tag="AC16UZ_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6245513.1"
                     /translation="MYRFLSAALVVLAAGCGAPQEAAEPSKPTYDDSRVGHFGYGKPA
                     SAEEIAGWDIDIRPDGLGLPQGSGSVEDGEWLYEAQCAECHGSFGEGVGRFPVLSGGK
                     GSLTDPRPSKTVGSYWPYTSTLYDYIRRTMPFTQPESLTADETYAVTAYVLYLNDLVE
                     DDFVLTHENLASVRLPNEGNFVPDPRPDVSNTRCMTDCRDPAAISVLSEVPTYVPASM
                     TMEEIAEAEPAHPGAATYDQYCSICHDVGVAGAPRIGDAAAWSSRLERGMAALEMNAI
                     NGIASEAGVMPAKGGFAHLADDEVNAAVAYMVEGSQ"
     gene            complement(1261..2574)
                     /gene="soxC"
                     /locus_tag="AC16UZ_02165"
     CDS             complement(1261..2574)
                     /gene="soxC"
                     /locus_tag="AC16UZ_02165"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017134966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HIR6245514.1"
                     /translation="MRQRQLDQIVKSVAESGDKLPREERRRFLRQGLSLAGATAAASF
                     AGGALADSGNLPPNVPSWTRRLGPGVLTNPYGQPSPFESHVQRRTVDWLTADRIAAIS
                     FTPLGDLNGIITPSGVVFERYHAGAPAIDPNQHRLMIHGMVKRPIILTMDDLMRFPSV
                     SMIRFLECPANGGMEWRGAQMDRLQFSHGMLACCEWTGVLLSTLLEEVGLEADASWIL
                     AEGADGAHMSRSIPMEKALDDALIVYAQNGEALRPEQGYPVRLLNPGWEGNTSIKWLR
                     RLEVGDKPWHHREETSKYTDLMADGTAREFTFVQECNSVVTHPCPERPLKGKGKYFIE
                     GLAWSGRGKIRHVDVSVDGGVTWREARFTSQVLPRALTRFGLEWDWDGSPALIQSRAV
                     DETGYVQPTYGQLKAVRGTNSIYHKNAIHTWRLEPNGTVSNVQIS"
BASE COUNT          449 a          900 c          933 g          524 t
ORIGIN      
        1 aacgtagtcg accacccggt cgatctcctc ctcggtcaga atcaggttgc gtccgtaggg
       61 cggcatgacc gaatccggat tgcgctcggt ggcgtcccag atctgcgctc gcagcacggc
      121 ccggtccggg aatctcaggc gcatctgcag caggggcggg ccgctgttgc ccggcatctc
      181 gccgtctccc atcatgtgac aggacaggca gttgccggca ttgcgatcag atgcgatggc
      241 cttgccccgc tccaggtcgt cgggggcctc ggccttggcc tggtccggcg ccaaaacgac
      301 cagcagggcg gttgtcagga ccagcgcggc tcgtgcaatc attgagaacc ctccaccata
      361 taggccaccg ctgcattgac ctcgtcgtcc gccaggtgcg cgaagccgcc ttttgccggc
      421 atgacgccag cctccgatgc gattccgttg atggcgttca tttccagggc cgccatgccg
      481 cgctcgaggc gcgatgacca ggcagcggcg tcgccgatgc ggggtgcgcc ggcgacaccg
      541 acatcgtgac atatgctgca gtactgatcg tacgtcgccg cgcccggatg ggccggttcg
      601 gcctcggcga tctcctccat ggtcatggag gccggcacgt aggttggaac ttcggagagc
      661 acgctgatgg ccgcggggtc gcggcaatcg gtcatgcagc gcgtgttgct cacgtccggc
      721 cgggggtccg gcacgaagtt gccctcgttg ggaagacgca cggaggccag attttcgtgg
      781 gtgagcacga agtcgtcttc gaccagatca ttcaggtaca ggacgtaggc ggtcaccgcg
      841 tacgtctcgt cggccgtcag ggactccggc tgcgtgaagg gcatggtgcg gcggatgtag
      901 tcgtacaggg tgctggtgta gggccagtag ctgccaacgg ttttgctggg ccgcgggtct
      961 gtcagcgagc cctttccgcc agacagcacg gggaagcgcc cgaccccttc gccgaaggat
     1021 ccgtggcact cggcgcactg cgcctcgtag agccactcgc cgtcctcgac ggatccgctc
     1081 ccctggggaa gtcccaggcc gtccggtctg atgtcgatgt cccaccccgc gatttcctcg
     1141 gcgctcgccg gcttgccgta gccgaagtgg ccgacgcggc tgtcgtcgta cgtcggcttg
     1201 gatggttcgg ccgcctcctg gggggcaccg cagccagcgg cgaggacgac gagcgcggca
     1261 ctaagaaatc tgtacattgc tcaccgtccc gttgggttcc agccgccagg tgtgaatggc
     1321 gttcttgtga tagatggagt tggtgccgcg cacggccttg agctgcccgt aggtgggctg
     1381 aacatagccg gtctcgtcga cggcgcggct ctggatcagt gcgggcgagc cgtcccagtc
     1441 ccactccagg ccgaatcgcg tcagcgccct tggcagcacc tggctggtga agcgcgcttc
     1501 ccgccaggtc acgcctccat ccaccgagac gtccacgtgc ctgatcttgc cgcggccgga
     1561 ccaggcgagc ccttcgatga aatactttcc tttgcccttg aggggccttt cggggcaggg
     1621 atgggtcacg acggagttgc actcctggac gaaggtgaac tcgcgtgcgg tgccgtctgc
     1681 catcaggtcg gtgtacttgg aggtctcttc gcggtgatgc cacggtttgt cgccgacctc
     1741 gagccgtcga agccacttga tcgaggtgtt gccctcccat cccggattca gcaggcgcac
     1801 cggatatccc tgctccggcc gcagggcctc gccgttctgg gcgtacacga tgagcgcatc
     1861 gtccagcgcc ttctccatgg ggatgctgcg gctcatgtgg gcgccgtccg cgccttcggc
     1921 caggatccag ctggcgtcgg cctccagccc gacctcctcg agcagggtgg agagcagcac
     1981 gcccgtccac tcgcagcagg cgagcatgcc gtggctgaac tgcagccggt ccatctgggc
     2041 gcctcgccac tccatgcctc cgttggcggg acattcgaga aaccggatca tgctcaccga
     2101 cgggaagcgc atcagatcat ccatggtcag aatgatcggg cgcttgacca tgccgtggat
     2161 catcaaccgg tgctggttcg ggtcgatggc cggcgcgccg gcgtgatagc gctcgaacac
     2221 cacgccgctc ggcgtgatga tgccgttgag atcgcccaga ggcgtgaagc tgatggcggc
     2281 gattcggtcc gccgtcagcc agtccacggt gcgccgctgg acgtgggatt cgaaggggga
     2341 gggttggccg taggggttgg tcagaacgcc cggacccagc cgccgggtcc atgacgggac
     2401 gtttggagga aggttgcccg aatccgcgag cgccccgccg gcgaagctcg cagccgccgt
     2461 ggctccggcg aggctcaaac cctgccgcag gaagcgtctt cgctcctccc gtggcagctt
     2521 gtcgccggac tcggcaacgg acttcacgat ctgatccagc tgacgttgac gcacgctccc
     2581 tccgcgtata ttttccgtcg cgcgatgcga agagttagta tattataaat ttcttaagta
     2641 aaggcaagcc gaaatatcgg ctggatttgg agggtcgaaa aacggcggcc cgccatcgga
     2701 gtattcgttg atggaactcg aagtcatgca ggaaaacgcc ggccgggcgg ccgacctgat
     2761 gaagctgctc gggcacccgc accggctcat gattctctgc cagctc
//
LOCUS       DBNHOX010000098         4238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_779553, whole genome shotgun sequence.
ACCESSION   DBNHOX010000098 DBNHOX010000000
VERSION     DBNHOX010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_779553"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /gene="dnaG"
                     /locus_tag="AC16UZ_02170"
     CDS             complement(<1..961)
                     /gene="dnaG"
                     /locus_tag="AC16UZ_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011336218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR6245515.1"
                     /translation="MAGRIPQSFINDLLNRVDIVDVIDARLQLRKAGRDYQALCPFHN
                     EKTPSFTVSPEKQFYHCFGCGESGTALTFLMEHDRMEFVEAVEALAQLVGVEVPREQG
                     GRPARDNTDLYDVLAAADRYFRKALRQSPEAIEYLKARGVTGTIARDFGIGYAPDEWQ
                     GAASALAGMGEAKLLEAGLLTRGEKGRAYDRFRGRIMFPIRDTRGRIIGFGGRVMADG
                     GGPKYLNSPETPVFHKGRELYGLYEARQALRRIDRLIVVEGYMDVVALAQAGISNAVA
                     SLGTAATEEHFQSLYRYTGEVICCFDGDAAGRQAAWRALESALP"
     gene            1066..1308
                     /locus_tag="AC16UZ_02175"
     CDS             1066..1308
                     /locus_tag="AC16UZ_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245516.1"
                     /translation="MRTTVRLPGELLTKAKEFAHERGTTLTALIEEGLIMVLERAPRA
                     GKMKPVRVPVSKSTGGLQPGVDLSDSAALLDAMEQG"
     gene            1305..1727
                     /locus_tag="AC16UZ_02180"
     CDS             1305..1727
                     /locus_tag="AC16UZ_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HIR6245517.1"
                     /translation="MKLADVNVLVGAFRTDSSHHERCRSWLKSTVNAPASFGLAAQVL
                     SGVIRVTTHPKVFVNPSPIDESIRFANFLLGRPNAIVVSPGERHWTIFTDLCRSGGIQ
                     GNLVPDAWFAALAMESGCEWVTLNGNFARFEGLSWSAP"
     gene            complement(1734..3158)
                     /locus_tag="AC16UZ_02185"
     CDS             complement(1734..3158)
                     /locus_tag="AC16UZ_02185"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HIR6245518.1"
                     /translation="MEKVIPVLLAGGTGSRLWPLSRELFPKQFHALLGDDSLLQSTLK
                     RADRTTGEAPIIVCNEEHRFMVAEQCRAIGQSWRRIILEPEGRNTAPAIALAALAACE
                     EAGPDSAPLLLVLPSDHLITDLDGFASAAELAARAAGEGALVTFGVVPTSPETGYGYI
                     EAPGAELGSGVVRVRSFVEKPDLATANEYLAAGDYFWNSGMFLLNARAYLDELGRHSP
                     LMLEKASLAFERGAEDLDFFRPGPEFLDCPADSIDYAVMEKTDRAMVVPAGFDWSDVG
                     SWSAIWEASDRDGNGNRLEGDVISVDTQNSFVSARDRLVGTIGVSDLVVVETSDAVLV
                     ADKDRVQDVRAVVEQLKAAGRDEYVHHREVFRPWGSYESVGAGERYQVKRIKVKPGAS
                     LSLQMHHHRAENWIVVRGTAEVTRGDETITLAENQSTYIPLGVTHRLANPGKLTLELI
                     EVQVGSYLGEDDIVRFDDIYGREG"
     gene            complement(3273..3740)
                     /locus_tag="AC16UZ_02190"
     CDS             complement(3273..3740)
                     /locus_tag="AC16UZ_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR6245519.1"
                     /translation="MSDLPISELRQAITAATTAAMKARDKQRLAVLRLVNADIKRAEI
                     DERRGASTDAEVLAVLNRMLKQRQDALEQYTQAGRKDLADQESFEIDLIGEFMPEQLS
                     EAELASAIDQAIAEANAESMRDMGKVMGLLKDRLQGRADMGAVSGRVKALLAG"
     gene            complement(3763..3978)
                     /gene="rpsU"
                     /locus_tag="AC16UZ_02195"
     CDS             complement(3763..3978)
                     /gene="rpsU"
                     /locus_tag="AC16UZ_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR6245520.1"
                     /translation="MPSVKVKENEPFDVALRRFKRSCEKAGVLSEVRRREYYEKPTSV
                     RKRKAAAAVKRHLKKLQREARRFEKSF"
     gene            4086..>4238
                     /locus_tag="AC16UZ_02200"
     CDS             4086..>4238
                     /locus_tag="AC16UZ_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341126.1"
                     /note="universal genome maintenance protein;
                     Kae1/Qri7/OSGEP/YgjD family protein; in Archaea, some Kae1
                     are found as fusion proteins similar to two distinct
                     proteins in yeast that are involved in the KEOPS complex;
                     kinase associated endopeptidase 1 (Kae1) and a
                     serine/threonine protein kinase (Bud32); in Pyrococcus
                     Kae1 has atypical AP endonuclease activity and inhibits
                     the kinase activity of Bud32; the protein is essential in
                     Escherichia coli and Bacillus subtilis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR6245521.1"
                     /translation="MLVLGIETSCDETGVALYDGVEGLLADALHSQVEVHAAYGGVVP
                     ELASRDH"
BASE COUNT          720 a         1415 c         1338 g          765 t
ORIGIN      
        1 ccggcagggc gctctccagc gcccgccacg ccgcctggcg cccggccgca tcgccgtcga
       61 agcagcagat cacctccccc gtgtagcggt aaaggctctg aaaatgctcc tcggtagccg
      121 ccgtgccgag gctggccacc gcgttggata tgcccgcctg ggccagcgcc accacgtcca
      181 tgtagccttc caccacgatc aggcggtcga tccggcgcag ggcctggcgc gcctcgtaga
      241 gaccgtagag ctcccggccc ttgtggaaga cgggggtctc ggggctgttg agatacttcg
      301 gccctccgcc atcggccatc acccgcccgc cgaagccgat gatgcggccc cgcgtgtcgc
      361 ggatgggaaa catgatgcgg ccacgaaacc ggtcataggc ccgacccttc tcgccccggg
      421 taaggagccc ggcttccagg agcttggctt cccccatgcc ggcaagcgcg ctcgcggccc
      481 cctgccactc gtccggcgcg tacccgatcc cgaagtcccg cgcgatggtg ccggtgaccc
      541 cccgggcctt gaggtactcg atggcctcag gcgactggcg cagcgccttg cggaagtaac
      601 gatccgcggc ggcgagcacg tcgtagagat cggtgttgtc ccgcgccggc cgcccccctt
      661 gctcccgcgg aacctcgacg ccaaccagct gggccagcgc ttccaccgct tccacgaact
      721 ccatgcggtc gtgctccatg aggaacgtca gcgccgtgcc gctctcgccg cagccgaagc
      781 agtgatagaa ctgcttctcc gggctcacgg tgaaggaggg cgttttctcg ttgtggaacg
      841 ggcagagcgc ctgatagtcg cgcccggcct ttctcagctg caggcgcgcg tcgatcacat
      901 ccacgatgtc cacccggttc aggaggtcat tgatgaagga ttgaggtatg cgaccggcca
      961 tcgcagtcag attagcaacg aattcttcgg gagtctgcaa tcggctgcct gacgacgcgc
     1021 tttaaatgtg atatgtctag acatacgaat cgcaggggac cagtcatgag aaccaccgtg
     1081 agactgccgg gagagctgct gaccaaagcc aaagagttcg cacatgaacg cgggacgacg
     1141 ctcacggcgc tgatcgagga gggcctcata atggttcttg aacgcgcgcc gagggcggga
     1201 aagatgaaac ctgttcgagt acccgtctcc aagtcgactg gcggcctgca gccaggagtg
     1261 gatctgagcg attcggccgc tctgctggat gccatggagc aaggttgaag ctcgccgacg
     1321 tcaacgtcct ggtgggcgcg ttccggaccg actcatcgca tcacgagaga tgccgaagct
     1381 ggctgaagag cacggtcaac gcgcccgcca gcttcgggct cgccgcgcag gtgctgtccg
     1441 gcgtcatccg cgttacgacg cacccgaaag tcttcgtaaa cccgagcccg atcgacgaat
     1501 caattcgttt cgcgaacttc ttactcgggc gaccgaacgc catcgtcgtc agcccaggcg
     1561 agcgtcactg gacgatcttc acggacctgt gccgatctgg cggtattcaa ggaaatctgg
     1621 tgcccgatgc ctggttcgcg gctcttgcca tggagtcggg gtgcgagtgg gtgaccctga
     1681 acgggaactt cgcccgtttc gagggcctca gctggagcgc gccctgagcg ccctcagccc
     1741 tcccggccgt agatgtcgtc gaaacggacg atgtcgtcct cgccgaggta ggagcccacc
     1801 tgaacctcga tgagctctaa ggtgagcttg cccggattgg ccagccggtg ggtcaccccg
     1861 agcgggatgt aagtgctttg gttttccgcc agggtgatcg tctcgtcgcc gcgggtgacc
     1921 tcggcggtcc cccgcaccac gatccagttc tcggcgcgat gatggtgcat ctgcagcgaa
     1981 aggctcgcgc ccggcttcac cttgatccgc ttgacctggt aacgctcgcc ggcacccacg
     2041 gactcgtagc tgccccaggg ccggaacacc tcgcggtgat ggacgtactc gtcgcggccg
     2101 gccgccttga gctgctcgac caccgcgcgc acgtcctgga cccggtcctt gtccgccact
     2161 aaaacggcgt ctgaggtttc caccaccacc aggtccgaga cgccaatggt gccaaccagc
     2221 cgatcgcggg ccgagacgaa agagttctgc gtatcgacgc tgatgacgtc gccctccagg
     2281 cggttgccgt ttccgtcccg atccgacgcc tcccagatgg cgctccagga acccacgtcg
     2341 ctccagtcga agccggccgg gaccaccatg gctcggtcgg tcttctccat caccgcatag
     2401 tcgatggagt ccgcggggca atcgaggaac tcgggccccg gtcggaaaaa gtcgagatct
     2461 tcggccccgc gctcgaaggc cagcgacgcc ttctccagca tcaaagggct gtgccggccg
     2521 agctcgtcca gatacgcccg ggcattgagc aggaacatgc cgctgttcca gaaatagtcg
     2581 cccgcggcga ggtactcgtt cgccgtggcg agatcgggct tctccacgaa ggatcggacc
     2641 cgcacgacgc cggaacccaa ctcggcgccg ggtgcctcga tgtagccgta gccggtctcc
     2701 gggctggtcg gcaccacgcc gaacgtgacc agtgcgcctt cacccgcggc ccgggcggcg
     2761 agctcggcag cagacgcgaa cccgtccagg tcggtaatca gatgatccga cggcagcacg
     2821 agcagcaacg gtgccgaatc gggccccgcc tcctcgcagg cggcaagggc agccagcgcg
     2881 atggccggcg ccgtgttgcg cccctcgggc tccaagatga tgcgccgcca ggactggccg
     2941 atggcccggc actgctcggc gaccatgaag cggtgttctt cgttgcacac gatgatgggc
     3001 gcttcgcccg tggtacggtc tgcccgcttg agcgtcgact gcagaaggga gtcgtcgccg
     3061 agaagggcgt ggaactgctt ggggaaaagc tctctggaga gcggccagag ccggctgccg
     3121 gttcctccgg ccagaagtac gggaatcact ttttccatcg ctcgctcgtc actccgcacg
     3181 acggcattgc cgcgccaaga taccagatcc tcggattatc accgtgggcg gatttgtaag
     3241 tgaggccccg gcgctgccaa ctaacctgct gactaaccgg ctaaaagcgc tttgacgcga
     3301 ccgctgaccg cgcccatgtc tgcgcggccc tgaagccggt ctttcagcag gcccatgacc
     3361 ttgcccatgt cgcgcatgga ttccgcattg gcctcggcga tggcctggtc gatggcgctc
     3421 gcaagctccg cctcggagag ctgctccggc atgaactcgc cgatcaggtc gatctcgaag
     3481 ctctcctggt cggccagatc cttgcgcccg gcctgggtgt actgctccag ggcatcctga
     3541 cgctgtttga gcatgcggtt gagcacggcc agcacttcgg cgtcggtgga ggccccacgg
     3601 cgctcgtcga tctccgctcg cttgatgtcc gcattgacca ggcgcagcac tgccaggcgc
     3661 tgcttgtcgc gcgccttcat ggcggccgtt gtggccgcgg tgatggcttg ccttaactcg
     3721 gagatgggga gatcggacac ggcgttcccc tgtcgtgcgg tgtcagaagg acttttcgaa
     3781 gcgccgggct tcccgctgca gcttcttcag atggcgcttg actgccgccg ccgccttgcg
     3841 ctttcgaacc gacgtcggct tctcgtaata ctctcgtctc cgcacctcgg aaagcacgcc
     3901 ggccttctcg caggaacgct tgaatcgcct gagagcgaca tcgaacggct cattctcttt
     3961 gacttttacg ctgggcatgt tgtgcgcgaa atcccctcga acctgacggt tgttgctgaa
     4021 aagggcgcgg attttactgg cgccggacgg tttttgcaaa acgccggaat cgttaccatc
     4081 cgcccatgct ggtcctcgga atcgaaactt cctgcgatga gacgggcgtc gcgctctacg
     4141 acggtgtcga ggggctgctg gcggacgctc tgcacagcca ggtggaagtg cacgccgctt
     4201 acggcggcgt ggtgcccgag ctggcctcca gagaccac
//
LOCUS       DBNHOX010000099         3573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_265127, whole genome shotgun sequence.
ACCESSION   DBNHOX010000099 DBNHOX010000000
VERSION     DBNHOX010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_265127"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..786)
                     /locus_tag="AC16UZ_02205"
     CDS             complement(298..786)
                     /locus_tag="AC16UZ_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HIR6245522.1"
                     /translation="MFYDGRFAAVATTMQIWVDADACPGPIKEILFRAADKRGVQLTL
                     VANHPMRVQKSAFIRLLQVEQGFDKADAFIADRVEAGDLVITADIPLAADVVEGGGAA
                     LNPRGTLYTTENIREHLARRNFMEELRSTGQISGGPAALGKADVQKFANSLDRYLTRA
                     VG"
     gene            complement(819..1499)
                     /locus_tag="AC16UZ_02210"
     CDS             complement(819..1499)
                     /locus_tag="AC16UZ_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245523.1"
                     /translation="MRPWRGTCGWCIGLVGVIAAAAFPAGAVQAAAISIAEARAAEPG
                     TLVTVEGSVTVPSGRFSASTFDQGFAIQDGSAGIYVSVADDPKLNNHRRVRVTGVVGD
                     DGYGQLVLRPGVDADVEILQGAARVAARQVATGDVGEATEGLLVEVTGSVVRGPTDDL
                     PYGYSVYIDDGSGETQIFIPASTGINPFRIGYLDEGVAIRVTGLSSQFQNNLEVLPRT
                     GGDIQRGR"
     gene            complement(1496..2554)
                     /locus_tag="AC16UZ_02215"
     CDS             complement(1496..2554)
                     /locus_tag="AC16UZ_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6245524.1"
                     /translation="MDYGIALATTMDSWRAARRAEELGFSHAWFYDTQLLNPDVFMGM
                     TLAAVNTSRIRLGTGVLIPSNRIEPVTANALVSLNKLAPGRIDFGVGTGFTGRRTMGL
                     KAIPLERMKSYIERVQALVKGETVTWDFEGETHDIAFLNPELGLINVDDPIALHVSAF
                     GPRSRDLTAELGAGWLNFGVSEPVAFGSLAKMRESWARAGRDAGDLYSTLFSLGCVLK
                     PGEAFDSPRAKAQAGTAVAVFLHDMAETSEPGSLEPVMGKAVSHVVEAYREIYLGYPE
                     GQRHLLNHRGHLMFLKPEEQDLITAELIQAITFTGERAALRERLDAFEAAGYGQFTVQ
                     LIEGHEDAIEDWAEVFFG"
     gene            complement(2568..>3573)
                     /locus_tag="AC16UZ_02220"
     CDS             complement(2568..>3573)
                     /locus_tag="AC16UZ_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR6245525.1"
                     /translation="GFLMGHQLSVAELRKRGKVVSLLALGKVVGAALVVFVAVWALTG
                     DLALALVLGGVATATAPAATFDVVHEAHARGAFADNLVSIVAIDDAWGLVLFSVLLAL
                     AASMANGDALDASIVGAGVMEVLGSLVLGVVIGVPMAYLTGRLTFGERQGEPIQAESF
                     GFVLACAGGAVLLDLSPILASMAMGSVVASLARHHARPFHAIEGVEWPFMILFFVLAG
                     ASLEVEGVSLVGWLALAYLVARALGTHLGIYLTAGIIGTDGPTRRWMGLALMPQAGVA
                     LGMALIASQRLPGHAETILTLVLSTTILLELVSPIITRRILERVGAGGELPDATDTTN
                     "
BASE COUNT          636 a         1240 c         1142 g          555 t
ORIGIN      
        1 caactgctcg aggccacaat tttggcctgc agtcacgacg actctttctg gcacgggcac
       61 aggcaccaca gataacactt ccgaacccgt tacggtagac aacgttacag tctcgaacac
      121 cgtctcaaac gggttcgccg ttgcgattgc cgacggcgtc aatccgtttg cggacctctg
      181 cctctaacca cctcttcggc atggtctccg gcagcatccg ctgtcgggga ccaagttttc
      241 cgtccgctac acacgtctga acctcaaccc gtcaaccatt tggtggctta cgccgaccta
      301 acccaccgcc ctggtcagat accgatccag cgagttcgcg aacttctgca cgtccgcctt
      361 gcccagcgca gccgggccgc ctgagatctg cccggtggag cgcagctcct ccatgaagtt
      421 gcgccgggcg agatgctcgc ggatgttctc ggtagtgtag agggtgccgc gggggttcag
      481 cgccgcgccg ccgccttcaa ccacatcggc cgccaggggg atgtccgcgg tgatcacgag
      541 gtcgcccgct tcgacccggt cggcgatgaa ggcgtccgcc ttgtcgaacc cctgctccac
      601 ctgcagcagc cggatgaagg ccgacttctg cacccgcatg gggtggttgg ccaccagggt
      661 gagctgcacc ccgcgcttgt ccgcagcgcg gaagaggatc tccttgatgg gcccggggca
      721 ggcgtcggca tccacccaga tctgcatggt cgtggcaacg gcggcaaagc ggccatcata
      781 gaacatccat agaacatcga ggaccggtgg cgttggcctt atctgccccg ttgaatatct
      841 ccaccggtgc gcggcagcac ttccagattg ttctgaaact ggctgctcag ccccgtgacg
      901 cgaattgcca cgccctcatc caggtagccg atcctgaacg ggtttatccc cgtgctggcg
      961 ggtatgaaga tctgcgtctc cccggagcca tcgtcgatgt aaacgctgta gccgtagggc
     1021 agatcgtccg tcggcccccg cacgacggat cccgtcacct ccaccagcag accctccgtg
     1081 gcctcgccca cgtcccccgt tgccacctgc cgggcggcca cgcgggcagc gccctggagg
     1141 atctccacat ccgcatcgac acccggccgc agcaccagct gcccgtagcc gtcgtccccg
     1201 acgacgccgg tgacccgcac gcggcgatga ttgttcagct tggggtcatc ggcaacggag
     1261 acgtagatcc ccgccgagcc gtcctggatg gcgaaaccct gatcgaacgt agacgcggaa
     1321 aaccggccgg acgggaccgt caccgagccc tccacggtca ccagggtgcc cggctccgcg
     1381 gcccgcgcct cggcgatgga gatcgcggcg gcctgaacgg cgcctgcggg gaaagcggca
     1441 gccgcaatga cgccgacaag cccgatgcac cagccgcagg tgccccgcca cggcctcatc
     1501 cgaagaacac ctccgcccag tcctcgatgg cgtcctcgtg gccttcgatg agctggacgg
     1561 tgaactggcc gtagccggcg gcctcaaacg cgtcgagccg ctcgcgcagg gccgcccgct
     1621 cgccggtgaa ggtgatggcc tggatgagct cggcggtgat gaggtcctgc tcctcgggct
     1681 tcaagaacat caggtgaccc ctgtggttca gaagatgacg ctggccctcc gggtagccga
     1741 gatagatctc ccgataagcc tccaccacgt ggctcacggc cttgcccatg acgggctcta
     1801 aagaacccgg ctcggaggtc tccgccatgt cgtggaggaa cacggccacc gcggtgcccg
     1861 cctgggcctt ggcccttggc gagtcgaacg cctcgccggg cttcagcacg cagcccagcg
     1921 aaaacagcgt gctgtagaga tcgccggcat cccgccccgc gcgggcccag gattcgcgca
     1981 tcttcgccag cgacccgaac gccaccggct cgctcacgcc gaagttcagc cacccggcgc
     2041 caagctctgc cgtcaggtca cgggagcgcg ggccgaaggc tgagacgtgc agcgcgatgg
     2101 gatcgtccac gttgatgagg ccgagctcgg gattgagaaa ggcgatgtcg tgggtttcgc
     2161 cctcgaagtc ccaggtcacc gtctcgcctt tgacaagcgc ctgaacgcgc tcgatgtacg
     2221 acttcatcct ctcgaggggg atcgccttca ggcccatggt gcgccggccg gtgaagccgg
     2281 tgccaacgcc aaagtcgatg cggccggggg caagcttgtt gaggctcacg agcgcgttgg
     2341 cggtgaccgg ctcgatgcgg ttgctgggta tcagcacccc ggtgccgagg cggatgcgcg
     2401 aggtgttcac cgccgccagc gtcatgccca tgaagacgtc cgggttcaag agctgggtgt
     2461 cgtagaacca ggcgtggctg aagccgagct cctccgcgcg ccgggccgcc cgccaggagt
     2521 ccatggtggt cgctagagca attccgtagt ccatgtttgc gtccgtttca gtttgtcgta
     2581 tcggttgcgt ccggcagctc gccacccgca ccgacgcgtt ccaggatccg gcgcgtgatg
     2641 atcggcgaga ccagctccag caggatggtg gtgctcagca ccagggtgag aatggtctcg
     2701 gcatggccgg gaaggcgctg agaggcgatg agcgccatgc cgagcgccac cccggcctgg
     2761 ggcatgaggg ccagacccat ccagcggcgg gtcgggccgt ccgtgccgat gatgcctgcg
     2821 gtaaggtaga taccgaggtg cgtgcccaac gcgcgggcca ccaggtacgc gagggcgagc
     2881 cagccaacca ggctgacgcc ttccacctcc agcgaggcgc cggccagcac gaaaaaaagg
     2941 atcatgaaag gccattccac cccctcgatg gcgtgaaaag ggcgcgcgtg gtgcctggcg
     3001 aggctcgcca ccacgctgcc catggccatg gaggcaagga tgggcgaaag gtcgagcagc
     3061 accgcgcccc ccgcgcaggc cagcacgaac ccgaaagatt ccgcctgaat gggctcgccc
     3121 tgccgctcgc cgaaagtcag ccgcccggtg aggtaggcca tgggcacgcc gatgacgacg
     3181 ccgaggacca ggctccccag cacctccatc accccggcgc caacgatgga ggcatccagc
     3241 gcgtcgccat tggccatgct ggccgccagc gccagcagca cggagaacag gacgaggccc
     3301 caggcatcgt cgatggcgac gatgctcacg aggttgtcgg caaacgctcc tctggcatga
     3361 gcctcgtgca ccacgtcgaa ggtggcggca ggcgcggtgg ccgtcgccac cccgccgagc
     3421 accagggcta gggcaaggtc tccggtcagg gcccacacgg cgacgaacac caccagcgcg
     3481 gcccccacca ccttgcccag cgccagcagc gagaccacct tgccgcgctt gcgcagctct
     3541 gcgacggata gctgatggcc catgagaaag ccc
//
LOCUS       DBNHOX010000100         3866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1036930, whole genome shotgun sequence.
ACCESSION   DBNHOX010000100 DBNHOX010000000
VERSION     DBNHOX010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1036930"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="AC16UZ_02225"
     CDS             <1..184
                     /locus_tag="AC16UZ_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HIR6245526.1"
                     /translation="HLAISCGFVLFACAAILWQTSAIIHGGETNYILATITLYVQIYN
                     LFISLLHIFGAFGSDD"
     gene            complement(204..2192)
                     /locus_tag="AC16UZ_02230"
     CDS             complement(204..2192)
                     /locus_tag="AC16UZ_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HIR6245527.1"
                     /translation="MTALVDVEEPLALFAEGITGRYYHIKAVGEYSGNRMVFDPARGA
                     LTGDALLLPETFEMDDPACYRVLALEQLSIVEFGTLKFSMAEARLRIPALEPPGEPPI
                     TDRASDLERFFNHVSRPHLLSRLFALCERVRLNAQVRDQYPGIRRHQDALHAVLRQSA
                     PRGDTSSVAAILDGLGYRVLGGRNAALESHPLAGALLELMLGVARLDADVFDSARAAH
                     ACYRLCMASAATAEAVAVLEEDLDDAASWLQRDVRLEDWEEGLKQMNGLILAAETAEG
                     EEAVAADVEGLSDGDIRPADVDLRALSNERDVLARRLDMERATIRDAVGRPRPDARSY
                     RYDEWDYLNRSYRTRWCRLFEERLAPDTQQDLDALRHVIRRHRRAVQSQLEHIKPQGY
                     QRVTRVADGDELDFNAVIEARGDIRAGLTPDERVYSRRERVHRDVCAAFLVDLSASTD
                     DPVDPPEAVAAGAVEELANLRDPYEFPVAHDEPAEEPRRIIDVQRESMLVMSAALEQL
                     ADSYGIYGFSGYGRECVEFFVAKEPDEPFTRGTLSAIAGMKPKRSTRMGPAIRHATSK
                     LLRSGHALKVLIILSDGFPQDSDYGPERGDHEYGVQDTAKALREAEEKGVETFCVTVD
                     RSGHDYLKRMCPNARYMVIEETRDLPGALSKVYEALTG"
     gene            complement(2189..3028)
                     /locus_tag="AC16UZ_02235"
     CDS             complement(2189..3028)
                     /locus_tag="AC16UZ_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6245528.1"
                     /translation="MSEAAKLARLAEEPYYLPLADEVAVFRSAYEARLPVLLKGPTGC
                     GKTRFVEHMAWRLYRDTQNPGAELEVPLVTISCHEDLTATDMVGRFLLQGDSTIWSDG
                     PLTRAVRTGAICYLDEIVEARKDTTVLIHSLTDHRRLLPIEKTGELVPAHPGFLLVIS
                     YNPGYQSVLKDLKTSTRQRFVSLTFDYPDREREAEIIAHESDVEPAVAERLAALGEKV
                     RNLREHGFEEGVSTRLLIYAAQLIERGIPSRRAVDAAVVQAVSDDATVQQAIADIVDA
                     VLP"
     gene            complement(3025..>3866)
                     /locus_tag="AC16UZ_02240"
     CDS             complement(3025..>3866)
                     /locus_tag="AC16UZ_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6245529.1"
                     /translation="SAEDVGNVQMLEHVNVTVPDQGLAALFYVTGLGFTRDPYIDFGT
                     HNMWVNLGEQQFHLPQSDAQILRGEIAVVVPNLEDLAKRLKFVSKALKDTRFSWEEAD
                     QRVVLSCPWGNRIVAYPAGSFPAMDLGMPYVDMSVPPGTAPGIARFYESVMGCPVSVE
                     PGRVKVAMGYNQKLIYTETEAPLAPYDGHHIAIYVSDFSGPHRFLHERGLISQESDQH
                     QYRFQKIVDPADDRGEAEPLFELEHEVRSLGHPMYRRPLVNRNPAVNFFTYRRGNEAF
                     TPG"
BASE COUNT          623 a         1247 c         1301 g          695 t
ORIGIN      
        1 ccacctggcc atatcctgcg gcttcgtgct gtttgcctgc gcggccattc tctggcagac
       61 gagcgccatc atccacggcg gcgagaccaa ctacatactc gccaccatca ccctctacgt
      121 gcagatctac aacctgttca tctcgttgct gcacattttc ggagcgttcg gcagcgacga
      181 ctgaacgccg cgacagatcc tgatcagccg gtgagcgctt cgtagacctt gctgagcgct
      241 cccggcagat cccgggtttc ctcgatgacc atgtagcgcg cgttagggca catgcgcttg
      301 aggtagtcgt gccccgagcg gtccaccgtg acgcagaacg tctccacgcc cttttcctcg
      361 gcctcccgga gcgctttggc ggtgtcctgc acgccgtatt catgatcgcc ccgctccggc
      421 ccgtagtcgg agtcctgggg aaagccgtcg ctcaggatga tcagcacctt cagggcgtga
      481 cctgagcgca gcagcttgga cgttgcgtgg cgtatggccg ggcccatgcg ggtgctgcgc
      541 ttgggcttca tcccggcaat ggcggacagg gtgccacgcg tgaacggctc gtccggctcc
      601 ttggcgacga agaactcgac gcactcgcgg ccatatccgg aaaagccgta gatgccgtag
      661 ctgtctgcga gctgctccag ggcggccgac atgacgagca tggattccct ctggacgtcg
      721 atgatgcgac ggggctcctc ggccggctcg tcgtgggcga ccggaaattc gtagggatcg
      781 cgcaggttgg cgagctcctc gaccgcgccg gccgcgacgg cttcgggcgg atccaccgga
      841 tcgtccgtgg acgcggacag atcgacgaga aaggcagcgc agacgtcccg gtgcacgcgt
      901 tcccggcggc tgtagacgcg ctcgtccggg gtaagccctg cgcggatgtc gccgcgggcc
      961 tcgatcaccg cgttgaagtc cagctcgtca ccgtccgcca cccgggtgac ccgctgatat
     1021 ccctggggct tgatgtgctc cagctggctc tgcactgccc ggcggtggcg gcggatcaca
     1081 tgccgcagcg cgtcgaggtc ctgctgtgtg tccggggcga ggcgctcctc gaacagccgg
     1141 caccatcgcg tgcggtacga gcggttcaga tagtcccact cgtcgtagcg gtagctgcga
     1201 gcatccggtc gaggcctgcc cacggcgtcg cgaatcgttg cccgctccat gtcgagccgt
     1261 cgggcaagga cgtcgcgctc gttgctcagc gcccgcaggt caacgtccgc agggcggatg
     1321 tcgccgtcgg agaggccctc cacgtccgca gcgacggcct cctcgccttc tgccgtctcg
     1381 gccgccagga tcaggccgtt catctgcttg aggccctcct cccagtcctc gagccggacg
     1441 tcccgctgaa gccagctggc cgcgtcgtcc aggtcttcct cgagcaccgc cacggcctcc
     1501 gcggtggcgg cggatgccat gcacagccgg tagcaggcgt gggcagcgcg tgccgagtcg
     1561 aagacgtcgg cgtccagacg agccacgccg agcatcaatt cgagcagggc gcctgccagc
     1621 ggatgggatt ccaaggccgc gttccgccca cccaggaccc ggtaacccag accgtcgaga
     1681 atggcggcga cggacgacgt gtcgccccgc ggcgcgctct gcctcagcac ggcgtgcagc
     1741 gcgtcctgat gccgcctgat cccggggtac tggtctctta cttgggcgtt gagccggacc
     1801 cgctcgcaga gggcgaaaag ccggctcagc aggtggggtc gggagacgtg attgaagaag
     1861 cgctcgaggt cgctcgctct gtccgtgatg ggcggctccc ccgggggctc taaagcgggg
     1921 atgcgaagtc ttgcttcggc catggagaat ttcagcgtgc cgaactccac gatgctcagc
     1981 tgctccaaag ccagcacccg gtagcaggcc gggtcgtcca tttcgaaggt ttcgggcagc
     2041 agcagcgcgt cgcccgtcaa cgcgccgcgc gccggatcga agaccatccg gttgcccgaa
     2101 tactcgccaa cggccttgat gtggtagtag cgcccggtta tgccctcggc gaagagcgcc
     2161 agcggctctt ccacatcgac cagggccgtc atggcagaac ggcgtcgacg atatcggcta
     2221 tggcctgctg caccgtggca tcgtcggata ccgcctggac caccgcggca tccaccgccc
     2281 ggcgggaagg gattccccgc tcgatgagct gggcggcgta gatgagaagg cgggtgctca
     2341 ccccttcctc gaagccgtgc tcgcgcaggt tgcggacctt ttcgcccagg gccgccagac
     2401 gttccgccac ggcgggctcg acgtcggatt cgtgggcgat gatctcggct tcgcgctccc
     2461 ggtccgggta atcgaacgta aggctcacga accgctgccg cgtgctcgtc ttcaggtctt
     2521 tcagcacgct ctggtaaccc gggttgtagg agatcacgag caggaagccc gggtgcgcgg
     2581 ggaccagctc gccggttttc tcgatgggga gcaggcggcg gtgatcggtc agcgagtgga
     2641 tcagcaccgt ggtgtccttg cgcgcctcga cgatctcgtc taagtagcag atggcgccgg
     2701 tccgaacggc ccgggtcagg ggcccgtcgc tccagatcgt cgagtctccc tgcaggagga
     2761 agcgacccac catgtccgta gcggtcaggt cctcgtgaca ggagatggtt accagcggca
     2821 cttcgagctc ggcaccgggg ttctgggtat cccggtacag gcgccaggcc atgtgctcca
     2881 cgaagcgcgt cttgccgcag ccggtcgggc ctttgagcag cacgggcaga cgagcctcgt
     2941 aggcggagcg gaagacggcc acctcgtcgg cgagcggaag gtagtagggc tcttctgcga
     3001 gccgggcgag tttggctgct tcgctcatcc gggagtgaag gcctcgttgc ccctgcgata
     3061 ggtgaagaag ttcaccgcgg gattccggtt caccagcggt cgccggtaca tgggatggcc
     3121 aaggctgcgg acctcgtgct ccagctcgaa cagcggctcg gcctcgccgc gatcgtcggc
     3181 gggatcgacg attttctgaa agcggtactg gtgctgatcc gattcctggc tgatcaggcc
     3241 gcgttcgtgc agaaagcggt ggggacctga aaaatcgctg acgtagatgg cgatgtggtg
     3301 accgtcgtag ggggcgagag gcgcttcggt ctccgtgtag atcagcttct ggttgtagcc
     3361 catggccacc ttgacgcggc cgggctccac ggataccggg cagcccatca ccgattcgta
     3421 gaaccgtgcg atgccagggg cggttccggg cgggacggac atgtccacgt agggcattcc
     3481 caggtccatg gcgggaaaag atccggcggg gtaggcgacg atgcgattgc cccagggaca
     3541 gctgagcacc accctttggt ctgcctcttc ccacgagaag cgggtgtcct tcaacgcctt
     3601 ggagacgaac ttcagtcttt tcgcgagatc ttcgaggttt ggaacgacca ctgcgatctc
     3661 gccgcgaagg atctgggcgt ccgactgggg gagatgaaac tgctgctcac cgagattgac
     3721 ccacatgttg tgcgtgccga agtcgatgta cgggtcgcgg gtgaagccga gaccggtcac
     3781 gtagaacagc gcggccagcc cctgatcggg aacggtcacg ttgacgtgct ccagcatctg
     3841 cacgttgccg acgtcctcgg cgctgc
//
LOCUS       DBNHOX010000101         2321 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1743666, whole genome shotgun sequence.
ACCESSION   DBNHOX010000101 DBNHOX010000000
VERSION     DBNHOX010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1743666"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="AC16UZ_02245"
     CDS             <1..1452
                     /locus_tag="AC16UZ_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6245530.1"
                     /translation="ACLVVSGPGVIHAFAGLANAQQNCWPMILIGGASPTYQNGMGAF
                     QEERQVELAKPYCKYAHGIDSVARIPYYVEQAIRTSLYGRPGPVYLDLPDDIIRGTVE
                     EDEVVSAAPVGPAPRSQARPEDVEAALCVLESAERPLVVVGKGMAWARAENEVREFIE
                     RTQLPFLASPMGKGVLPDDDPLSVGAARSTALKEADVVFLMGARLNWIMHFGLPPRFA
                     PDVRIIQLDIAAEEIGTNVPAEVALLGDGQAVAAQLNAALDNRPWFYPADAPWRTLLR
                     EKASDNQAAVAPMIDAEAMPMNYYRAYRDIEAWLPEDAIIIGEGANTMDIGRTQMANR
                     SARNRLDAGTYGTMGIGLGFTIAAAVVHPDRPVVAVQGDSAFGFSGMELETACRYKLP
                     IKVIVLNNGGIGAGIDELPEDGPIPPFSLTPGARYEGIMETLGGRGLYVEDPKDLRGA
                     LDEAMAFDGPALVNVRIDPRAGRKQQQFGWLTT"
     gene            complement(1454..>2321)
                     /locus_tag="AC16UZ_02250"
     CDS             complement(1454..>2321)
                     /locus_tag="AC16UZ_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6245531.1"
                     /translation="CALEPKIEGLVEECLATVQGGMFDLMDTLARPLPANVIADILGV
                     PREDRARFQAWSELIAKNGRNIEYDALQQTDAVFNELIDYLAQITDAKRADPGDDLIS
                     HLIAAEEDGDRLSADEIHATSALLLIAGHETTTRLIGNAMWLLLSHPEQLEILRANPA
                     LMPNAVEEALRFEAPVQHMTRIALEDMDFHGVHIRKHQLCEVYIGSANRDPQANPNPD
                     VFDICRENIQHVGFGYGIHLCLGAQLARLETQMALTMLLERFPRLALTGAPPAWGDST
                     FVRGLDELLLAA"
BASE COUNT          390 a          781 c          773 g          377 t
ORIGIN      
        1 gcatgtctgg tggtttcggg tcccggcgtg atccacgcct tcgccgggct ggcgaacgcc
       61 cagcagaact gctggcccat gatcctcatc ggcggcgcct cccccaccta ccagaacggc
      121 atgggcgcgt tccaggaaga gcgccaggtg gagctggcca agccctactg caaatatgcc
      181 cacggcatcg acagcgtcgc gcggattccc tactacgtgg agcaggccat tcgaacgtcg
      241 ctctacggcc ggcccgggcc cgtctacctc gacctgcccg acgacatcat tcgcggcacg
      301 gtggaggagg acgaagtcgt cagcgccgcg cccgtgggcc cggcgccccg ctcccaggcc
      361 agacccgagg acgtggaggc agccctctgt gtgctcgaat cggccgagcg gccactcgtc
      421 gtggtcggca aagggatggc atgggcgcgc gccgagaacg aggtgcgcga gttcatcgag
      481 cggacccagc tgccgtttct cgcctccccc atgggcaagg gcgtgctccc ggacgatgat
      541 cccctttcgg tgggagccgc gcgcagcacg gcgctcaagg aggccgacgt ggtcttcctg
      601 atgggcgcgc gcctgaactg gatcatgcac ttcggcctgc caccgcggtt cgctcccgac
      661 gtgcggatca ttcagctgga catcgccgct gaggagatcg gcaccaacgt ccccgcggag
      721 gtggcgctgc tgggcgacgg ccaggccgtg gccgcccagc tcaacgccgc tttggacaac
      781 cgcccgtggt tctatcccgc cgatgccccg tggcggacat tgctgcggga gaaggcttcg
      841 gacaaccagg cagccgtggc gcccatgatc gacgccgagg ccatgccgat gaactactac
      901 cgggcctacc gcgacatcga ggcgtggctt ccggaagacg cgatcatcat cggggaaggg
      961 gccaacacca tggacatcgg ccgcacccag atggcgaacc ggtcggcccg caaccgtctg
     1021 gacgcgggca cgtacggaac catgggcatc ggcctgggct tcaccatcgc ggccgccgtc
     1081 gttcatccgg accggcccgt ggtggccgtg cagggcgaca gcgctttcgg tttctccggc
     1141 atggagctcg agaccgcctg ccgctacaag ctgcccatca aggtcatcgt cctcaacaac
     1201 ggcggcattg gcgccggcat cgacgagctg cccgaagacg ggcccatccc gccgttctcg
     1261 ctgaccccgg gcgcgcgcta tgagggcatc atggagacgc tcggcgggcg cggcctttac
     1321 gtggaggatc ccaaagacct ccggggcgcg ctggacgagg ccatggcctt cgatggcccc
     1381 gccctcgtga atgtacgcat cgatccgcgg gccggccgca agcagcagca gttcggctgg
     1441 ctgacgacct gaatcaggcg gccagcagaa gctcgtcgag gccgcggacg aaggtgctgt
     1501 caccccaggc ggggggcgcg cccgtcaggg cgagccgggg aaaacgctcc agcagcatcg
     1561 tcagcgccat ctgcgtctcg agccgcgcaa gctgggcccc caggcaaagg tggatgccgt
     1621 agccgaagcc cacgtgctga atgttctcac ggcagatgtc gaagacgtcc gggttcggat
     1681 tcgcctgggg atcgcgattc gctgagccga tgtacacctc gcacagctgg tgcttgcgga
     1741 tatgcacgcc gtgaaagtcc atgtcctcca gagcgatccg cgtcatgtgc tgcacgggcg
     1801 cttcgaaccg aagcgcttcc tcgacggcgt ttggcatgag ggccggattc gcgcgcagga
     1861 tctcgagctg ctccggatgg ctgagcagca gccacatggc gttgccgatc aggcgggtgg
     1921 tggtttcgtg accggcaatc agcagcaggg cgctggtggc atgtatctca tccgcggaca
     1981 gccggtcgcc atcctcttcg gcggcaatca gatggctgat cagatcgtcg cccggatcgg
     2041 cccgcttggc gtcggtgatc tgcgcaagat agtcgatcag ctcgttgaaa acggcgtcgg
     2101 tctgttgcag ggcatcgtac tctatgttgc ggccgttctt cgcgatcagc tccgaccagg
     2161 cctgaaacct tgcgcggtct tcccgaggca cgcccaggat gtcggcaatc acgttagccg
     2221 gcaggggacg cgccagggta tccatgagat cgaacatacc gccctggacc gtcgccaggc
     2281 actcctcgac caggccctcg atcttcggct ccagcgcgca g
//
LOCUS       DBNHOX010000102         4660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1615128, whole genome shotgun sequence.
ACCESSION   DBNHOX010000102 DBNHOX010000000
VERSION     DBNHOX010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1615128"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="AC16UZ_02255"
     CDS             <1..290
                     /locus_tag="AC16UZ_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6245532.1"
                     /translation="FPRYLSTGEQQRVGIARAVATRPRILLADEPTGNLDPELSRDIM
                     HVFEQVNQVGTTVIIASHDRDLIESMGLRIIELAHGNIVRDTDREADIGFE"
     gene            277..1293
                     /locus_tag="AC16UZ_02260"
     CDS             277..1293
                     /locus_tag="AC16UZ_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HIR6245533.1"
                     /translation="MASNNEAQGGRPAPATGGGRGRTKKGYPWPHRRVGLVAWWQDHL
                     RTGRETLHFISARLGTSLLVWLLVGIALALPAGLILLQLNLAGMTADWDGRPGLSVYF
                     ELNTDAGKVAAIAQRLSERAEIEGVRVTSPEEALAEFQSHADVGDALDLLTENPLPAS
                     LRATLVPGSSLLDLELVAGLVEGAPGVSEVVVEKTWLERVIDISRVVSRLGIILGALF
                     GVGAVLVTATSVRLAIEARLEELRVLKLVGATDRQIRRPFMYFGALYGLGGGLVAAML
                     ISLCLVAIETPVSNLLGSYGRDLETPGFDPTFLSGLLLVGAFLGVSGALLAARQRLKD
                     LEIL"
     gene            1384..2247
                     /gene="rpoH"
                     /locus_tag="AC16UZ_02265"
     CDS             1384..2247
                     /gene="rpoH"
                     /locus_tag="AC16UZ_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HIR6245534.1"
                     /translation="MSTDVLSMKSLAPGRDLDAYVQTVSAFPVLSAEQEKDLAERFYY
                     DNDLEAARELVLCHLRFVVHLARSYRGYGLPQADLIQEGNVGLMKAVKRFNPEVGVRL
                     VSFAVHWIKAEMHEFILRNWRIVKVATTKAQRKLFFNLRSSKKRLAWLTQEETRAMAR
                     ELGVSEEEVARMEGRMAASDLAFDGYASDDDSSPLAAPVHYLEHEGSNPEDLVAEEDW
                     HDEASARLGAALAELDERSRAIVEARWLAEKKATLHELGDTYGVSAERIRQIEQNAIK
                     KLKAACMSDAA"
     gene            2270..2644
                     /locus_tag="AC16UZ_02270"
     CDS             2270..2644
                     /locus_tag="AC16UZ_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HIR6245535.1"
                     /translation="MPANVPLAAGALFGLTGVVMGALGAHGLAGQLSESGVDIWDTAV
                     LYHLTHALALVAVGVRLGQKVPPPWPVVLAGWGFGVGILLFSGSLYVLALGGPPVFGP
                     LTPLGGLALCAGWVALLAAARR"
     gene            2664..3344
                     /gene="trmB"
                     /locus_tag="AC16UZ_02275"
     CDS             2664..3344
                     /gene="trmB"
                     /locus_tag="AC16UZ_02275"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIR6245536.1"
                     /translation="MEERTYLRRRGRMTRGQARALDRAGDTMIVDSTSPDWPAVFGRQ
                     APLGVEVGFGMGHALLDWAAECPDWNLVGIEVYQPGIGALLLGLDSQGSTNVRVLEAD
                     ARQALAAGFEPDSIDEVRILFPDPWPKKRHHKRRLVQPDFVRLLASRMRPGARLGLAT
                     DWQDYAQHMLTVLDAEPLLQNRSGQGSYAERPSGRPTTRFEERGRRLGHQVWDLGYER
                     KRETTESR"
     gene            complement(3308..4411)
                     /gene="hemW"
                     /locus_tag="AC16UZ_02280"
     CDS             complement(3308..4411)
                     /gene="hemW"
                     /locus_tag="AC16UZ_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR6245537.1"
                     /translation="MGPVGLYAHFPWCVRKCPYCDFNSHPVRGSIDEAGYVSALLADL
                     RKNLPRDVPIGSVFFGGGTPSLFHAPAFAAVLEEVASQLTEDAEVTMEANPGTTEHSD
                     LRAYRQAGINRLSIGAQSFDDEALERLGRIHHAAETLDCAARARSAGFENINIDLMYG
                     LPHQTPDQAVQDLEAALALEPDHISWYQLTIEPRTEFARRPPPIPPEDRIDETEQRGR
                     DRLEQAGFERYEVSAYARPGRRSRHNVNYWSFGDYVGIGAGAHGKLTSGGRVARTVKP
                     SQPRLYLQDPESQQRQIVEAEALPFEFMLNALRLRDGVPLDQFTQRTGLALEALDPVW
                     GELVDEGLVHRHRLATTPEGYRYLDSVVSRFLS"
     gene            complement(4411..>4660)
                     /locus_tag="AC16UZ_02285"
     CDS             complement(4411..>4660)
                     /locus_tag="AC16UZ_02285"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HIR6245538.1"
                     /translation="SFHCAVVFMRAPDDPVPVVATAAWSGRIVADPRGTGGFGYDPHF
                     FIDETDCTAAELDPSVKNEISHRGRAMRALCAALAGVV"
BASE COUNT          755 a         1432 c         1619 g          854 t
ORIGIN      
        1 tgtttccgcg ttacctttcc accggtgagc agcagcgggt tggcattgcc cgggcggtgg
       61 ccacacggcc gaggatcctt ctggcggacg agcccacggg caatctggat cccgagctct
      121 cccgggacat catgcacgtc ttcgagcagg tcaatcaggt cggcaccacg gtgatcatcg
      181 cgagccacga tcgagacctg atcgagtcca tggggctgcg catcattgag cttgcccacg
      241 gcaacatcgt cagagatacg gacagagaag cggacattgg cttcgaataa tgaggcgcaa
      301 gggggtcggc ccgcgccggc cacgggcggc gggcgaggcc gcacgaagaa gggatatccc
      361 tggccccacc gccgcgtggg gctggtggcc tggtggcagg accatctgcg cacgggacgg
      421 gagacgctgc atttcatcag cgcccgtctg ggaacgagcc tgctggtctg gttgctggtg
      481 gggatcgcgc tggctctgcc cgcggggctg attctgcttc agctcaacct cgccggcatg
      541 acggccgatt gggatgggcg accggggctg tctgtgtatt tcgagctgaa caccgacgca
      601 ggaaaggttg cggcgattgc ccaacggctg tccgaacgtg ccgagatcga gggtgtcagg
      661 gtgacctcgc ccgaggaagc gctggctgaa tttcaaagtc acgcggacgt tggagacgct
      721 ctcgacctgc tgacggagaa cccgttgccg gcctcgctgc gcgccaccct ggtgccgggg
      781 tcttcgctgc tcgatctgga gctggtcgcg gggcttgtcg agggagcgcc gggagtatcg
      841 gaggtggtgg tcgagaagac ctggctggag cgggtcatcg acatttcccg ggtggtcagc
      901 cgcctgggga tcattcttgg ggcgcttttc ggtgtcggcg cggtgctggt caccgcaact
      961 tccgtgcggc tggccatcga ggcgcggctg gaggagctca gagtgctgaa actggttggg
     1021 gccaccgacc gacagatccg tcggcccttt atgtacttcg gcgcgcttta cgggcttggt
     1081 ggcggtctcg tggccgccat gctgatcagc ctttgcctgg ttgccataga gacaccggtc
     1141 tcgaatctcc tcggaagcta cggccgtgac cttgaaacgc cggggttcga cccgaccttc
     1201 ctttccgggc tgctgctggt cggcgctttc ctgggagtgt ccggggcgct tctggctgcg
     1261 cggcagcgtc tgaaggatct cgaaatcctt tagttttcgg cgggttaggg aaccattcgc
     1321 ggggttagca gtctcaccac cagagtgcta aactgctgaa attagtgtta ccggaggtat
     1381 cacatgagca ccgacgtgct ttctatgaaa agtctcgccc cgggacgcga tctggatgct
     1441 tacgttcaga cggtcagcgc gtttccggtt ctttctgcag aacaggagaa ggatctggcg
     1501 gagcggtttt actatgacaa cgacctggaa gccgcccggg aactggtgct ctgccatctg
     1561 cgcttcgtcg tccacctggc ccgttcgtac agaggctacg ggctgcctca ggcggacctg
     1621 atccaggagg gcaacgttgg cctgatgaag gccgtcaagc ggttcaatcc cgaggtgggc
     1681 gttcgcctgg tctcttttgc cgtgcattgg atcaaggcgg aaatgcacga gttcatcctg
     1741 cgtaactggc ggatcgtcaa ggttgctacc accaaggccc agcgaaagct cttcttcaac
     1801 ctgcgcagct ccaagaagcg gctcgcctgg ctcacccagg aagagacccg tgccatggcc
     1861 cgggagctgg gcgtttccga ggaagaggtc gcgaggatgg agggccggat ggcagcgtcc
     1921 gacctggcct tcgacggcta tgcttcggac gacgactcct cgcccctggc cgcccccgtc
     1981 cactatctgg aacacgaagg ttcgaatccc gaggacctgg tggcagagga agactggcac
     2041 gacgaagcca gtgcgcgtct gggcgccgcg ctggccgagc tggatgagcg aagccgggca
     2101 atcgtggaag cccgatggct cgccgagaag aaggcgacgc tccacgagtt gggcgacaca
     2161 tacggcgtgt cggcggagcg gatccgccag atcgagcaga atgcgatcaa gaagctgaaa
     2221 gccgcgtgta tgagcgacgc cgcctgatcc gctgggcatc gggggtacga tgccggccaa
     2281 cgttccttta gccgcgggag ccctcttcgg cctgaccggc gtagtgatgg gcgcccttgg
     2341 cgcccacggg cttgccgggc aactgtctga aagcggcgtc gatatctggg acaccgccgt
     2401 gctctaccat ctcacgcacg ccctagccct cgtcgccgtg ggcgtgcgcc tgggccagaa
     2461 ggtgccgccg ccgtggccgg tcgttctggc cggctggggg tttggtgtcg gcatcctgct
     2521 attctccggc agcctttacg tgctcgctct gggcgggcca cccgtcttcg gccctctgac
     2581 gcccctgggt ggcctcgcgc tctgtgccgg ctgggtcgcc ctgctcgccg ccgcccgccg
     2641 ctgagcgccg ctcgggacac gcattggaag aaaggactta cctgcgccgc cgcgggcgga
     2701 tgactcgggg tcaggctcgg gccctcgacc gagcgggcga cacgatgatc gtcgactcga
     2761 catcgcccga ctggcctgcg gtgttcggcc ggcaagcccc gctgggggtg gaggtcggct
     2821 tcgggatggg acatgccctg ctggactggg cggccgagtg cccggattgg aacctggtgg
     2881 ggatcgaggt ctatcagccg gggataggcg ctctgctgct gggcctggac tcccagggct
     2941 cgaccaacgt tcgcgtgctg gaagcggacg cgaggcaggc gctggctgcg ggctttgagc
     3001 ccgactcaat cgacgaggtg cggatactct ttccggatcc ctggcccaag aaacgtcacc
     3061 acaagcgccg gttggtgcag cccgatttcg tccgtctcct tgcgtcgaga atgcggcctg
     3121 gggctcggct cggcttggct accgactggc aggactatgc ccagcacatg ctgaccgttc
     3181 tcgatgcgga gccgttgctg cagaaccgat cggggcaggg ttcctatgcc gagcgccctt
     3241 cgggtcgccc gaccacgcgc ttcgaggagc gtggccggcg gctcgggcat caggtgtggg
     3301 atcttggcta cgaaagaaag cgcgagacca ccgagtccag gtagcgatag ccctcggggg
     3361 tggttgcgag gcgatggcgg tgaacgaggc cttcatccac tagctcgccc cagaccggat
     3421 cgagagcctc cagggcaagc cccgtgcgct gggtgaactg gtccaggggg acaccatcgc
     3481 ggaggcgaag cgcgttgagc atgaactcga agggcagagc ttcggcttca acgatctgcc
     3541 gctgctggct ttcggggtcc tgcaggtaaa ggcgcggctg gctgggcttt accgtgcggg
     3601 ccacccgacc accgcttgtc agcttgccgt gggcgcccgc tccgatcccg acgtagtcgc
     3661 cgaacgacca gtagttcacg ttgtgacgcg atcgccgacc tggcctggcg tacgctgaga
     3721 cctcgtagcg ctcgaagcct gcctgctcga gccgatcacg accccgttgc tccgtctcgt
     3781 cgattcggtc ctccggggga atcggggggg gtcgccttgc gaactcggtg cgcggctcga
     3841 tggtgagctg ataccagctg atgtgatccg gttccagggc cagggcggcc tcgaggtcct
     3901 gaactgcctg gtccggagtc tggtgcggga gaccgtacat caggtcgatg ttgatgttct
     3961 cgaacccggc cgatctggcg cgcgcggcgc aatccagggt ttcggcggca tgatggatgc
     4021 gcccaagcct ctcgagcgcc tcgtcgtcga acgactgcgc tccgatggac aggcggttga
     4081 tgcccgcctg ccgataggcg cgcaggtctg aatgctcggt ggttccgggg ttggcttcca
     4141 tggtcacctc ggcgtcctcc gtcagctggg aggcgacctc ctcgaggacg gcggcgaacg
     4201 ccggggcatg gaacaggctg ggcgtcccac cgccgaagaa gacgctgccg atgggcacgt
     4261 cgcgcggcag gttctttcgc aggtcggcaa gcagcgcgct cacgtagccg gcctcgtcga
     4321 tagaccctcg aacggggtga gaattgaaat cgcagtacgg gcacttgcgc acgcaccagg
     4381 gaaagtgggc gtacaacccg accggcccca tcaaacgact ccggccagcg cggcgcacag
     4441 cgcgcgcatg gcccgaccgc ggtggctgat ctcgttcttc acggacgggt ccagctcggc
     4501 ggccgtgcaa tccgtctcgt cgatgaagaa atgcggatcg taaccgaacc cgcccgtgcc
     4561 cctgggatcg gcaacgatgc gaccgctcca ggcggccgtg gcgacgacgg gtacagggtc
     4621 gtcgggtgcg cgcatgaaga ccacggcgca gtgaaagctt
//
LOCUS       DBNHOX010000103         2683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_843700, whole genome shotgun sequence.
ACCESSION   DBNHOX010000103 DBNHOX010000000
VERSION     DBNHOX010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_843700"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..356)
                     /locus_tag="AC16UZ_02290"
     CDS             complement(60..356)
                     /locus_tag="AC16UZ_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245539.1"
                     /translation="MISKSCYVYIQMPGSLEIATCGRFQLDHGEDGTSIGRFIYGQSY
                     LQRSDAVPLDPYHLPLVPREFTTVRLGGLFGALRDVAPDLWGRRVIERQMGEAD"
     gene            complement(1094..2050)
                     /locus_tag="AC16UZ_02295"
     CDS             complement(1094..2050)
                     /locus_tag="AC16UZ_02295"
                     /inference="COORDINATES: protein motif:HMM:NF040322.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR6245540.1"
                     /translation="MTTHHIAFWNVENLFDVRTSPRRSDKLQRTIGKELKGWTQAVLD
                     TKLRQLAKVITWMNGGQGPDILGVCEVENAHVLQLLVDRLAGLGRDYGIAHHDGEDGR
                     GIDVAFIYDKDRFQAEETFSHYIVKRTATRDLFQVNFRTKPGGNLLVLVGNHWPSRMG
                     GQLESEPYRIIAGETLAYFHEGIRRALDSKDVAVMAMGDFNDEPSDRSLTGYALAERE
                     RGRVTRARSPKFLNLMWPLMGTGQATHYYGSEGSMLDQIMVSKGLLKKDLPLRVKTDS
                     AAVLRHDEHAPKGVPVRYGRGSTRNEKGFSDHFPVCVEVEER"
BASE COUNT          431 a          899 c          861 g          492 t
ORIGIN      
        1 tcttcggccg ggcgcctcct accgagctgc cgggccggag taggtcttcc agctcggcct
       61 caatcggcct ctcccatttg acgttcgatg acgcgtcgtc cccagaggtc gggggcgaca
      121 tcccgaagcg caccaaagag cccaccgagc ctcacggttg tgaactcacg ggggacgagg
      181 ggaaggtggt acggatcgag cgggacggcg tccgaccgct gaaggtaact ctggccgtag
      241 atgaatcgcc ctatcgaagt gccgtcttct ccgtgatcca gttggaagcg accgcacgtg
      301 gcgatctcca gagatcctgg catctggatg tagacgtagc aggacttaga aatcattcgg
      361 caggactccg ggcttccgca cgcgctcacc acgggccgct gcctccaacg ttcggccctc
      421 aagatcgtgc gccggatcgg ctattctgga tacttcatcc tcgagaccga gcgcccagag
      481 cgctgcgatg taagccccga ggcccgttcc caggtaccct tcttcgaccc ttcgcagggt
      541 cggcacggtg acaccggctt gcatcgacag atcctcttgg cggatctgcc tgcgcaaccg
      601 agcagtacgg atgttggagc cgaggcgccg ggcgagaacg acaatcttcc tcggggcgga
      661 cttctctgat acggcgactc gaggcatggc cttcctccat acgtcctgta tcgaaactat
      721 aacgattttt aagtgactta aagcaaatta tatttgctat accataccgt gtggagcgcg
      781 cgttcgggat actgaggacc attcggtccc gctcgggctg gggctcacgg cgaatggcgt
      841 gacgccgggg ctcgtaccgg gcctctgctc gtgtcgatcc ccgctgtcgg gctcgtttcc
      901 gactcgcggg agcggcgcgc aatcgctcct gacctgggcg ttggcagcac tcgacgatgg
      961 actggggctg cttcgcggcc agcctccgcg tggctcgaag ccgcagccgc agcccgcggc
     1021 gcaggccggc cccgcgggcc gccccctcga cgtccgggct cgggcggcga acgcccggat
     1081 gtcgccgggc ctcctagcgt tcctccacct ccacgcacac cgggaagtgg tcgctgaagc
     1141 ccttttcgtt gcgcgtgctc ccccgcccgt accgcacggg cacgcccttg ggcgcatgct
     1201 cgtcgtgccg cagcaccgcg gcgctgtcgg tcttcacccg tagcggcagg tccttcttga
     1261 gcagcccctt ggagaccatg atctggtcca gcatggagcc ctcgctgccg tagtagtgcg
     1321 tcgcctgacc tgtgcccatg aggggccaca tgaggttcag gaacttcggc gagcgcgccc
     1381 gcgtcaccct gcctcgctcc cgctcggcca gggcgtaccc ggtgagagac cggtccgacg
     1441 gctcgtcgtt gaagtcaccc atggccatca cggccacgtc cttgctgtcc aacgcgcgac
     1501 ggatgccctc gtggaagtag gccagcgtct ctccggcgat gatgcggtac ggctccgact
     1561 ccagctgccc gcccatgcgt gacggccagt ggttgcccac caggacgagc aggttccctc
     1621 ccggcttggt gcggaagttc acctggaaga ggtcccgggt ggcggtgcgc ttcacgatgt
     1681 agtgactgaa ggtctcctcg gcctggaacc tgtccttgtc gtagatgaag gccacgtcga
     1741 tgccgcggcc gtcctcgccg tcgtggtgcg cgatgccgta gtcgcgcccg aggccggcga
     1801 gccggtccac gagaagctgc agcacgtggg cgttctcgac ctcgcagaca ccgaggatgt
     1861 ccgggccctg ccccccgttc atccacgtga tgaccttggc gagctgccgg agcttggtgt
     1921 cgaggaccgc ctgcgtccaa cccttgagct ccttgccgat ggtccgctgg agcttgtcgg
     1981 agcggcgggg ggaggtgcgg acgtcgaaga ggttctcgac gttccagaag gcgatgtggt
     2041 gcgtggtcat gaatccctcc cggaagatgg agccgggggg ctcatcgtct tcacgatgcg
     2101 cccgccgaca cgcgcatcca gggatcatgg cggctacgcc gcatccgcgc caccgtgaaa
     2161 tgtttcggcg ccaccgtgaa atgtttccgc gccaccgtga aattgtttcg ggcccacctc
     2221 gagacgtttc gggcggaaga gaacgcggag cggccgctcg aagagcgcgg ccagggtgaa
     2281 gagcgtgcga cggctctcga gcgggagaac cggcgcccga aggagctgat cgctcagatc
     2341 ccgacggaga gttcccccgg gagcttcaga atattaccga gaccccgggc gactcattga
     2401 tcgtccgact cgaaaggtct caatagggtg cgtgcgagca gcctccaccc gcctctcgcc
     2461 gcagggcgct ctcgtatcgc gcgcatcgca tacagcgccg tggtccgacg ttgcttgcca
     2521 taccatgccc aatcgccgta cctgagcatg tgttcgtacc tcaaagccgg atccgggccg
     2581 ccgggccgcc agttcaaaga gcccttgaat tcacctcggg tgccgcggcg ctcccaggca
     2641 tcctcgcacg cagctcgcgc tgcctcgcgt tgtctgaggc ctg
//
LOCUS       DBNHOX010000104         8503 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_972668, whole genome shotgun sequence.
ACCESSION   DBNHOX010000104 DBNHOX010000000
VERSION     DBNHOX010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8503)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8503)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8503
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_972668"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /gene="tpiA"
                     /locus_tag="AC16UZ_02300"
     CDS             <1..680
                     /gene="tpiA"
                     /locus_tag="AC16UZ_02300"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR6245541.1"
                     /translation="MARVVEDAVSVVVFPPAAYLGALVRAFEGSAIEVGGQNAHMESS
                     GAFTGEVAPEMVADVGGRWLLVGHSERRQYFGESDDLIADKYAAALRACLAPVLCVGE
                     TLEEREAGEAERVVCRQLAAVTERLDIAEVARGVVAYEPVWAIGTGRTATPGQVQEMH
                     RVIREALSERGAKLAEGMRILYGGSVNADNAGELMAQPDIDGGLVGGASLSADGFAAI
                     VAAAAGR"
     gene            739..1095
                     /gene="secG"
                     /locus_tag="AC16UZ_02305"
     CDS             739..1095
                     /gene="secG"
                     /locus_tag="AC16UZ_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR6245542.1"
                     /translation="MTTFETILLMLLVVDAIALAALVLLQQGRGADVGAAFGSGSSNT
                     MFGSAGSASFLTKLTVWLAIGFFLISFGLAYTAKERAAIIDEVGVPSVAPAEETVVPE
                     QLTPEPESVDSDIPDV"
     gene            1104..1190
                     /locus_tag="AC16UZ_02310"
     tRNA            1104..1190
                     /locus_tag="AC16UZ_02310"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Leu,seq:gag)
     gene            1310..1386
                     /locus_tag="AC16UZ_02315"
     tRNA            1310..1386
                     /locus_tag="AC16UZ_02315"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Met,seq:cat)
     gene            1648..2118
                     /gene="rimP"
                     /locus_tag="AC16UZ_02320"
     CDS             1648..2118
                     /gene="rimP"
                     /locus_tag="AC16UZ_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR6245543.1"
                     /translation="MNAKEKQIEALLTPTVEGLGCQIWGVEYASQGRHSRLRVYIDRE
                     EGVSVDDCERVSRHVSDVLDVEEVISGGYTLEVSSPGMDRVLFKAEQYAANVGQHVDV
                     RLVNPFEGRKRIVGVLAGLEEDDSCGHVIVQVDDEQYLLPLESVQRTRIIPQYD"
     gene            2134..3651
                     /gene="nusA"
                     /locus_tag="AC16UZ_02325"
     CDS             2134..3651
                     /gene="nusA"
                     /locus_tag="AC16UZ_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR6245544.1"
                     /translation="MSKEMLLVVDTVSNEKGVPKDVLFGAVEAALATATKKRYDDDAE
                     FRVAIDRESGEYETFRVWTVADPEALEEEEEWNPAAQLTVEEARERDANLQLGDVIEE
                     PVESVGFGRIAAQTAKQVIVQKVREAERAQVVEAYLPRVGELVNGTVKKLGRDSVIVD
                     LGNNAEGLLTREHLIPRETFRVGDRLRALLLEVNPDNRGPQLMLTRTSPKMLIELFKL
                     EVPEIAEDVIEIRGAARDPGSRAKIAVKTNDGRIDPVGACVGMRGSRVQAVSGELAGE
                     RIDIVLWDDNPAQLTVNAMAPAEVAAIVLDEDTHAMDIAVTEDNLAQAIGRGGQNVRL
                     ASDLVGWSLNIMTEEDAAAKQEEEAVRYIEEFMRALEVDEDVATVLVEEGFTSIDEVA
                     YVPIEEMTAIEGFDEEIVEELRNRAKDALLTKAIASEELLGEKEPADDLLNMEGMERR
                     LAFQLASNDIITMEDLAELSVPDFIEIVPEVDEETAAALIMTARAPWFEEAEAEG"
     gene            3677..6211
                     /gene="infB"
                     /locus_tag="AC16UZ_02330"
     CDS             3677..6211
                     /gene="infB"
                     /locus_tag="AC16UZ_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR6245545.1"
                     /translation="MSEVTVKQLADTVGAPVDRLLKQMQDAGLPHDSDDAAVTEEEKQ
                     TLLAYLKRSHGSDDAAPKKITLKRKTVGTLKSGQGRTGRTVNIEVRRKRTYVKRDEAE
                     TAGAAAEAPAKPASQSELEAQRIREQELARKEAEEETRRKEAERKAEEEKRVEEIKRQ
                     EEADLQARETKPGEAPAEDSAAKAAAAAAEAQAKAQASERGGKRVRGKERERPGEKRD
                     EFGDGKGRRRELSLKTQRRGKRRQPAPMPVEQQGGEFRPTDFIAREVEIPDIITVGEL
                     AQRMTVKAGEVIKALMGMGVMATINQSVDQDTATLIVEELGHKPKPIHEDAMEQALEA
                     SLQIEGDAAKRAPVVTVMGHVDHGKTSLLDYIRKTRVTSGEAGGITQHIGAYSVDTPH
                     GKISFIDTPGHAAFTAMRARGARVTDIVILVVAADDGVMPQTEEAIQHAKAAGVPMVV
                     AINKMDLEGADPDRVTNELAAKEVVPEDWGGDTQFVKVSAVSGEGIDELLEALLLQAE
                     ILELTAVEDAPGRGVVIESRLDRGRGPVSTVLVQNGTLKQGDVIIAGECHGRVRAMLN
                     DKGEQVKAAGPSVPVEVLGINGTPAAGDDFSVATDERTARELAEFRRDKSQEQRVATQ
                     HAAKLENMFANVGAGEKRVLKMVLKADVRGSLEAITQALTEMGNEEVAVNVLGSGVGG
                     ITETDANMALTYGAAVFGFNVRADNSAKAIIEREGLDLRYYSVIYELLDDVKQVLSGM
                     LSPEVREEIVGIAEVRDVFRSPRFGQVAGCMVTEGTVYRNKPIRVLRDNVVIYEGELE
                     SLRRFKDDVNEVRSGTECGIGVRNYNDVRVGDLIEVFDTKEVAREL"
     gene            6250..6669
                     /gene="rbfA"
                     /locus_tag="AC16UZ_02335"
     CDS             6250..6669
                     /gene="rbfA"
                     /locus_tag="AC16UZ_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR6245546.1"
                     /translation="MREFGRDLRVADFVRDELAKIIQREMRDPRVGMVSVNEVTVSRD
                     LAFADVYVSSIEARTAKEREELIGVLNKAAGFFRSELAKRHSMRTTPKPRFHYDEMVE
                     RGPELEALIDFAVGSDTRDEDPGTEDELGEGEGRGRG"
     gene            6662..7582
                     /gene="truB"
                     /locus_tag="AC16UZ_02340"
     CDS             6662..7582
                     /gene="truB"
                     /locus_tag="AC16UZ_02340"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR6245547.1"
                     /translation="MGRRGRGRDVSGVLVVDKPAGWSSNDAVQKAKRLFNARKVGHTG
                     ALDPLATGVLPLCFGEATKFSQYLLSSDKKYEVRIKLGVATDSGDADGNVVAEREVDD
                     VTPAGVEEALRFFRGEIDQVPSMFSAIKHQGQPLYKLARQGIEVERQARRVTVYSNEL
                     ISLEGDVVELHIHCSKGTYVRTIAQELGEVLGCGAHVSALRRTMAGPYGESDLVTFAE
                     LESSLEAEGTLDRFLQPISSTVREWPEVRLTDNTAYYLRQGQPVMVPHAPARGWVRLA
                     EIVGEDSTRFLGVGEVLDDGRVAPRRLVVG"
     gene            7648..7917
                     /gene="rpsO"
                     /locus_tag="AC16UZ_02345"
     CDS             7648..7917
                     /gene="rpsO"
                     /locus_tag="AC16UZ_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR6245548.1"
                     /translation="MALSAESKADIIKEYQIEEGDTGSPEVQVALLTHNINSLQEHFR
                     SHNKDHHSRRGLIRMVSQRRKLLDYLRSKDASRYSELIKRLGLRR"
     gene            7987..>8503
                     /locus_tag="AC16UZ_02350"
     CDS             7987..>8503
                     /locus_tag="AC16UZ_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR6245549.1"
                     /translation="MNPVTKEFQFGDQTVVLETGRIAKQATGAVLVTMGDTVVLCTVV
                     GAKSARPGQDFFPLTVNYQEKTYAAGKIPGGFFRREGRPSEKETLTCRLIDRPIRPLF
                     PKGFLNEVQVICTVMSTDKDQDPDIAALLGTSAALAISGLPFNGPIAAARVGYLDGRY
                     LMNPGYAALESS"
BASE COUNT         1721 a         2391 c         2943 g         1448 t
ORIGIN      
        1 tcatggcccg ggtcgtggaa gatgcggtga gcgtagtcgt gttccctccc gcggcctatc
       61 tcggcgcgct ggtccgggcc ttcgaagggt ctgccataga ggtcggtggg cagaatgcac
      121 acatggagtc ctcgggggct ttcaccggcg aggtcgcccc ggaaatggtg gccgacgtcg
      181 ggggtcgctg gctgttggtc ggtcattccg agcggcgcca gtatttcggc gagtcggacg
      241 acctgatcgc tgacaagtat gccgccgcgt tgcgagcgtg ccttgccccg gtgctatgcg
      301 tgggcgagac gctcgaggaa cgcgaagccg gggaggccga gcgggtcgtc tgcaggcagc
      361 tcgccgccgt cacggagcga ttggacatcg ccgaggtggc ccggggcgta gtggcttacg
      421 agccggtatg ggccatcggc acgggtcgca cggcgacgcc tggccaggtc caggagatgc
      481 atcgggtcat tcgcgaagcg ttgagcgagc gcggtgcaaa gctggcggaa gggatgcgga
      541 ttctctacgg tggcagcgta aacgccgata atgccggcga gctgatggcc cagccggata
      601 tcgacggcgg gctggtcgga ggcgcatcgc tcagcgcgga cgggttcgcg gcgatcgttg
      661 ctgcggcggc tggaaggtga cggaacagtg ggcctgctcc ggggcaggcg gtgagcagaa
      721 aaatcggtaa cgtgccacat gacaactttt gaaacgattc tgttgatgct gctcgttgtg
      781 gacgccatcg cgctggcagc gctggtgctg ctgcagcagg gccgtggcgc ggacgtaggc
      841 gcggcattcg gcagcgggtc ctccaacacc atgtttggca gtgccgggtc ggcttcgttc
      901 ctgacgaaac tgacggtgtg gctggccata ggctttttcc tgatttcttt cgggctcgcc
      961 tacaccgcca aggagcgcgc ggccattatc gacgaggtcg gggttcccag cgtggctcct
     1021 gctgaggaaa ctgtcgtccc ggagcagttg actccggaac cggaaagtgt agattccgac
     1081 atccccgatg tttgaggggt tctgccgaag tggtgaaact ggtagacacg ctaccttgag
     1141 ggggtagtgg gcgtacgccc gtggaggttc gagtcctctc ttcggcacca ttcccgttcc
     1201 agctcggccc ggcaaaaatg tcgtttaaaa ccgcgatgtt gcgtggtgcg gcccgatatt
     1261 taccggttgc ggggggcatg agccatcaat ataatgcgcc acttgctgat gcggggtgga
     1321 gcagtctggt agctcgtcgg gctcataacc cgaaggtcgt gggttcaaat cctgcccccg
     1381 ctaccaaagg ataaaagccc ctcctgtgct ttgcacgaaa aggtcagggg ctttttatta
     1441 cgccgggggg cgcgtcttcc cggcgtaggc gcagcgggcc ttatgaaccc gccatgacat
     1501 ccttgtaggt ggcccgccgc gtaccggctg ggccgcccgt ggtctgatgc gaaagaagcg
     1561 atctcgcgca attgcatcgg gtatgagaat gggcgtaagc ccatttttta ttggctgaag
     1621 gaaagcctga cggcgtttga caagctgttg aatgcgaagg agaaacagat cgaagcgttg
     1681 ctgacgccca cggtagaagg gctgggctgc cagatctggg gggtcgagta cgcctcccag
     1741 ggtcggcatt cgcgtctgcg tgtctacatc gaccgcgagg aaggcgtttc ggtggacgac
     1801 tgcgagcgtg tgagccgaca tgtcagcgac gttctggatg tggaagaggt gatatccggc
     1861 ggctatacgc tggaggtgtc ctctccgggc atggatcggg tcctgttcaa ggcggagcag
     1921 tacgcggcga acgtcggcca gcacgtggat gtccgtctcg taaatccgtt cgaggggcgc
     1981 aaacggatcg taggggtgtt ggcaggcctg gaggaagacg attcctgcgg acacgtaatc
     2041 gtgcaggtgg atgatgaaca gtacctgttg ccgctggaga gcgttcagcg caccaggatt
     2101 attccccagt atgactgagg tgcgaccaaa gttatgagca aagagatgtt gttggtggtg
     2161 gataccgtct ccaacgagaa gggcgtcccg aaggacgtgc ttttcggcgc cgtggaggca
     2221 gcgctcgcga ctgccaccaa gaagcgctac gacgacgacg ccgagttccg cgtcgccatc
     2281 gaccgggagt ctggagagta cgagacgttc agagtctgga ccgtggcgga tccggaggcg
     2341 ctggaagaag aggaagagtg gaaccccgcg gctcagctga ccgtggagga agcccgggaa
     2401 cgggatgcga atcttcagct cggcgatgtg atcgaggagc cggtggagtc ggtgggcttt
     2461 ggccgtatcg ctgcgcagac ggccaagcag gtcatcgtgc agaaggtccg tgaggccgag
     2521 cgtgctcagg tcgtcgaagc ttacctgccg cgggtcgggg agctcgttaa cggaaccgtg
     2581 aagaaactcg ggcgtgacag cgtcatcgta gatctcggca acaacgccga gggcctcctg
     2641 acccgggaac acctgattcc ccgggaaacg tttcgtgtgg gtgaccggtt gcgcgcgctt
     2701 ctgctcgagg tcaatccgga caatcgcgga cctcagctca tgctgacgcg cacctcgcca
     2761 aagatgctca tcgagctttt caagctggag gttcccgaga tcgccgagga cgtcatcgag
     2821 atacggggcg cggcgcggga tccggggtcc agggcgaaga tcgcggtgaa gaccaacgac
     2881 ggtcggatcg atccggtagg cgcctgcgtc ggcatgcggg gctcccgcgt gcaggccgta
     2941 tccggtgagc tggccgggga gcggatagac atcgttcttt gggacgacaa cccggcccag
     3001 cttaccgtca acgccatggc gccggccgag gtggccgcca tcgtgctcga tgaggacacc
     3061 cacgccatgg atatcgcggt caccgaggac aacctcgctc aggcgatcgg ccgcggcggg
     3121 cagaatgtgc gtctggccag tgatctggtc ggctggtcgc tgaacatcat gacggaagag
     3181 gatgcggcgg cgaaacagga agaggaggcc gtgcgctaca ttgaggagtt catgcgcgcc
     3241 ctcgaggtcg acgaggacgt cgccaccgtg ctggtggaag aaggtttcac ctccatcgac
     3301 gaggtcgcct acgtgcccat cgaggagatg acggccattg aagggttcga cgaggagatc
     3361 gtcgaggagc tgcgcaaccg ggccaaggat gctctgctga cgaaggcgat cgccagtgag
     3421 gagctgctcg gcgaaaagga gcctgcggac gatctgctga acatggaagg catggagcga
     3481 cgcctggcgt tccagctggc cagcaatgac atcatcacga tggaagatct tgctgaactt
     3541 tcggtgcccg atttcatcga gatcgtcccg gaggtcgacg aagagaccgc ggctgctctc
     3601 atcatgacgg cccgcgcgcc ctggttcgaa gaagcggagg cggaagggta agccgccccg
     3661 aagaggaaaa agagtcatgt cggaagtcac agtcaagcaa ctcgcagata cggtgggggc
     3721 gcccgtagac cgtctgctca agcagatgca ggatgccggg cttccacacg acagcgacga
     3781 cgcggcggtc acggaagagg agaagcagac gctgctcgcc tacctcaagc gcagccacgg
     3841 atctgacgac gccgcgccaa agaagatcac gctgaagcga aagaccgtcg gaaccctcaa
     3901 gtccggccag ggcaggacgg gccgtaccgt gaacatcgag gtgcgccgta agcgcaccta
     3961 cgtgaagcgg gacgaggccg agaccgcggg cgcggcggca gaagcgccgg caaagccggc
     4021 cagccagtct gagctcgagg cgcagcgtat tcgggagcag gaactcgctc gcaaagaggc
     4081 cgaagaggaa acccggcgca aggaagcgga gcgcaaggcc gaagaggaaa agcgggtaga
     4141 ggagatcaag cgtcaggagg aagccgacct tcaggcgcgc gaaaccaaac ccggcgaggc
     4201 tccggcagaa gactcagcgg cgaaagcggc cgctgccgct gcagaggccc aggcgaaggc
     4261 ccaggcaagc gagcgcggcg ggaaacgcgt acgcggcaag gagcgcgagc ggccgggcga
     4321 aaaacgcgac gagttcggcg acggcaaagg ccgccgccgg gagctctcgc tcaaaacgca
     4381 gcggcgtggc aaacgtcgtc agcccgcacc gatgcccgtc gagcagcagg gcggggaatt
     4441 ccgaccgacg gacttcatcg cgcgggaggt cgagataccc gacatcatca cggtgggcga
     4501 gctggcccag cgcatgacgg tgaaggcagg cgaagtcata aaagcgctca tgggtatggg
     4561 cgtgatggcg acgatcaacc agagcgtcga tcaggatacc gccacgctca tcgtcgagga
     4621 gctgggccac aagccgaaac ccatccacga ggatgccatg gagcaggctc tggaagcctc
     4681 gctgcagatc gagggcgatg ccgcgaagcg ggcacccgtg gtgacggtca tggggcacgt
     4741 cgaccatggc aagacctcgc tgctggacta catccgcaag actcgggtca ccagcggcga
     4801 agctggtggg atcacccagc acatcggcgc ctacagcgtg gacacgccgc acggcaagat
     4861 cagcttcatc gatacgcccg gtcatgccgc ctttaccgcc atgcgggctc gtggcgccag
     4921 ggttacggac atcgtcatcc tggtcgtggc tgcggacgac ggcgtcatgc cccagaccga
     4981 ggaagcaata cagcacgcca aagcggccgg cgtgcctatg gtcgtggcca tcaacaagat
     5041 ggacctcgaa ggcgccgacc cggaccgggt cacgaacgag ctggcggcca aggaagtggt
     5101 tcccgaggat tggggcggcg acactcagtt cgtcaaggtc tcggcggtct ccggcgaagg
     5161 cattgacgag cttctcgagg cgttgctcct gcaggcggaa atcctggagc tgacggccgt
     5221 ggaggatgct cccgggcgcg gcgtcgtcat cgaatcgcgg ctcgatcgcg gtcgggggcc
     5281 ggtgtccacc gtcctggtgc agaacggcac cctcaagcag ggggacgtga tcattgcggg
     5341 cgaatgccac ggccgcgttc gtgccatgct caatgacaag ggtgagcagg tgaaagcggc
     5401 ggggccgtcg gttcccgtcg aggtgctcgg catcaacggg accccggcag ccggtgatga
     5461 tttctcggtt gcaaccgacg agcgcaccgc gcgggagctc gccgagttcc ggcgggacaa
     5521 gagccaggaa cagcgtgttg cgacgcagca cgctgccaag ctcgaaaaca tgtttgccaa
     5581 cgtgggagcc ggagagaagc gcgttctcaa gatggtcctg aaagccgacg tgcgcggcag
     5641 cctcgaagcc attacccagg ccctcaccga aatgggtaac gaggaggtgg ctgtcaacgt
     5701 tctgggatcc ggtgtcggtg gcatcaccga aaccgacgcg aacatggcgc tcacctacgg
     5761 cgcggcggtc ttcggattca atgttcgagc agacaactct gctaaggcca tcatcgagcg
     5821 ggaagggctg gatctgcgct actacagcgt gatctacgag cttctcgacg acgtcaagca
     5881 ggtgctttcc ggcatgctct cgccggaggt gcgcgaggag atcgtgggga tcgccgaggt
     5941 tcgggacgtg ttccgttcac cgcggtttgg tcaggtcgcc ggctgcatgg tcaccgaagg
     6001 tacggtgtat cgcaacaaac ccattcgcgt cctgcgcgac aacgtcgtga tctacgaagg
     6061 ggagctggaa tcgctgcggc gcttcaagga tgacgtcaac gaggttcgca gcggtacgga
     6121 atgtggcatc ggcgttcgta actacaacga tgttcgcgtt ggtgatctca tcgaggtctt
     6181 cgatacgaaa gaagtggcgc gggagctcta ggatgcgccg ggtaattgag cctgtgtggg
     6241 ccgggatgca tgcgtgagtt tggacgtgat ctgcgggttg ccgatttcgt gcgcgacgag
     6301 ctcgctaaga tcatccagcg tgagatgcgg gacccccgcg tgggcatggt cagcgtgaac
     6361 gaagtcactg tcagcagaga cctggcgttc gcggacgttt atgtgtcctc catagaagca
     6421 cggaccgcca aggaacgcga agagctgatc ggcgtcctca acaaggcagc aggttttttt
     6481 cgctcggagc tggccaagcg tcacagtatg cggacgacgc cgaagccccg attccactac
     6541 gacgagatgg tcgaaagagg tccggagctg gaagccctta tcgacttcgc cgtgggttcc
     6601 gatacgcggg acgaggatcc tggtacggaa gacgagctcg gtgaagggga ggggcggggc
     6661 cgtgggtaga cggggtcgcg gcagggacgt cagtggcgtg ctcgtggtcg acaaacctgc
     6721 gggatggagc tccaacgacg ccgtgcagaa ggcaaagcgc ctgttcaacg cgcgcaaggt
     6781 cgggcacacg ggcgctctcg acccgcttgc caccggcgtg ctgccgctct gttttggcga
     6841 ggctacgaag ttctcccagt acctgctctc ctcggacaag aagtacgagg ttcgaatcaa
     6901 actgggcgtt gccacggata gcggcgacgc cgacgggaac gtggtggcgg agcgggaagt
     6961 cgacgacgtc acgcccgccg gggtggagga ggcgctgcga ttcttccgcg gggagatcga
     7021 tcaggttccg tcgatgttct ccgccataaa gcaccaaggg caacctcttt acaagctggc
     7081 ccggcagggg atagaggtcg agcggcaggc gcgtcgcgtg accgtctact ccaacgagct
     7141 catcagcctg gaaggcgacg ttgtcgagct gcacatccac tgcagcaagg gcacgtacgt
     7201 acgaaccatt gcccaggaac tcggagaggt gctcggatgc ggggcccacg tcagcgcgct
     7261 gcgtcgcacc atggccggcc cctacggcga gtcggacctg gtgaccttcg cggagctgga
     7321 gtcgtcgctc gaggcggagg ggaccctcga tcggttcctg cagccgatct cgagcaccgt
     7381 tcgggagtgg cccgaggttc ggctgacgga caacacggcc tattatctgc gacaggggca
     7441 gcccgtgatg gtgccccacg ccccggccag aggctgggtc cgtctggcag agatcgttgg
     7501 tgaggactcg acccgctttc tgggggtcgg ggaagtattg gacgatggcc gggtggcgcc
     7561 acgccgcctg gtcgttggtt agttgagttg actgtaaata tgcacggtca tcttgaatgc
     7621 aaacatgcat attgatggag aaaccaaatg gcactaagcg ctgaaagcaa agctgacatc
     7681 atcaaggagt atcagatcga ggagggggat accggatctc ccgaggtgca ggttgcactg
     7741 ctcacacaca acatcaattc gcttcaggag cattttcgct cccacaacaa ggaccatcat
     7801 tcccgaaggg gtctgatccg gatggtcagc caacgccgca agctcctcga ttatctcagg
     7861 agcaaagacg cgtcgcgtta ctcggagctc atcaagcggc tcggcctgag acggtaatcc
     7921 ggacaacccg gtacggggcc aaagattgat acaggtagaa atagagaagg aaacggagaa
     7981 atcattttga atccagttac gaaagaattt cagtttggtg atcagacggt ggtcctggag
     8041 accggccgca tcgccaagca ggccaccggc gcagtgctgg tgacgatggg agatacggtt
     8101 gtcctgtgca ccgtggtcgg tgcgaaatcc gcgcggccag ggcaggactt cttccccctc
     8161 acggtgaact accaggagaa gacctacgcc gcgggcaaga ttcccggcgg cttcttccgc
     8221 agggaaggtc ggccaagcga gaaagaaacg ctcacttgcc gcctgattga ccggccgatc
     8281 cgaccgctct tcccgaaggg gttcctcaac gaggtgcagg tgatctgcac ggtcatgtcg
     8341 accgacaagg atcaggatcc cgacatcgct gccttgctcg gaacgtcggc cgccctggcc
     8401 atatcgggcc tacccttcaa cggaccgatc gcggcagccc gcgtcggtta cctcgatggc
     8461 agatacctca tgaatcctgg ctacgctgcg ctcgagtcct cgc
//
LOCUS       DBNHOX010000105         4660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_8467, whole genome shotgun sequence.
ACCESSION   DBNHOX010000105 DBNHOX010000000
VERSION     DBNHOX010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_8467"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1293)
                     /locus_tag="AC16UZ_02355"
     CDS             complement(208..1293)
                     /locus_tag="AC16UZ_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HIR6245550.1"
                     /translation="MSSLLQFLLGLVLLVAGAETLVRGASKLAAAAGVSPLVVGLTIV
                     AFGTSSPELAVSVQSSWRGQGDLALGNVVGSNIFNVLFILGVSALVTPLIVARQVIRQ
                     EVPVMILASVLLFVIAMDGRIGRLDAAVLAALLLAYTVFLVVQSRAHARMDTDDETGV
                     QQDSGWDRHWTIQIALVLGGLCLLVLGSDMLVNAAIDFARAFGVSELVIGLTVVAAGT
                     SMPEVATSVMASLRGHRDIAVGNVIGSNVFNILGVLGISGLVAPGFLEVAPSALAFDL
                     PVMVAVALLCLPIFFTGSVITRGEGVLFLACYVAYTVFLILVAQGSPLLSIYRTLLIW
                     VLMPVTFAVVLLVTIGAARGSRGRGAA"
     gene            1953..2588
                     /locus_tag="AC16UZ_02360"
     CDS             1953..2588
                     /locus_tag="AC16UZ_02360"
                     /inference="COORDINATES: protein motif:HMM:NF018712.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HIR6245551.1"
                     /translation="MIDWLVQGESFSNCNCDHWCPCQFEGLPTHNYCQGVEVFRVDRG
                     HFGDVDLSGVTVALFFTWPGPVFEGKGTMQAVIDPSATPEQRDAISRIVKGAETREGA
                     NHWWVFHAMSDTILEDLSLPIEFSVDREARTARVFIPGYLEATGEPLRNPFNGASHRV
                     QIRCPDGIEFETADIGNASSTITGPISMTLKNSYGQFTYLDQSGNGPAHVQ"
     gene            2595..3386
                     /locus_tag="AC16UZ_02365"
     CDS             2595..3386
                     /locus_tag="AC16UZ_02365"
                     /inference="COORDINATES: protein motif:HMM:NF021465.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HIR6245552.1"
                     /translation="MSDVLERALRRDRAIVVTALALVTVLAWAYLLAGAGMGMSAMEM
                     TRHSMMPMPMMQHVQWGFSYAVLMFFMWWIMMIAMMLPSATPVILLAAGINRRSRLDQ
                     APYGSAAAFTTGYLLVWALFSLLATGAQWGLETLGWLTPMMVSASDLLAGAILVVAGV
                     WQFSPWKSACLSHCRSPVQFLTERRRAGNIGATMMGMHHGLFCLGCCWFLMGLLFVGG
                     VMNLYWIVGLAIYVAAEKLLPGGPRTAKVFGVLLIGWGVGVLAGL"
     gene            complement(3629..4027)
                     /locus_tag="AC16UZ_02370"
     CDS             complement(3629..4027)
                     /locus_tag="AC16UZ_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245553.1"
                     /translation="MSRIAVFLTLAGTFAALSATTAMADSGSCKYSDLTDLHTYLHSA
                     QFHATDDLGNYSEDLSAYGTPLPTAGAHAAPGPDSDEPPALDMVKLRSYFHGRAFQSP
                     DGLTNYGADYRAYETPSQSGQVARIAQNRP"
     gene            4241..>4660
                     /locus_tag="AC16UZ_02375"
     CDS             4241..>4660
                     /locus_tag="AC16UZ_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR6245554.1"
                     /translation="MQPLRIALLGGFELHAAGGEPISLPSRNARALLAYLAMSNGRAQ
                     ARDKLATLLWGGKSASQARASLRQALSQLRRTLNSVRSDILKIDGDAIVLDPSAVAVD
                     AVRLQELVSAGTPQALEESAALYHGDLLEGIGPFSESF"
BASE COUNT          849 a         1471 c         1507 g          833 t
ORIGIN      
        1 acatcagtca gagcaagctc aacgccatcg ctcgccggct gaacgaacgt ccgcggcaga
       61 ccctcggctt cgagacacca gcagaacgct ttggccaatg tgttgcctcg accagttgaa
      121 tcggcaaccc taagcggaca tcgcgatgat gcccgaaccg ggttcggtcc gaaggtctgc
      181 taacgaccca tcaccgacat tttccggtca cgccgctccc ctgcctctcg agccacgcgc
      241 ggccccgatc gtcacgagca aaaccaccgc gaaggtgact ggcatcaata cccagatcag
      301 gagggttcga tagatggaca gtagcgggct tccctgtgct accagaatca ggaaaaccgt
      361 gtaggcaacg taacaggcga ggaacagcac gccctcgccg cgggtgatta cgctaccagt
      421 gaagaagatc ggtaggcaca ggagtgccac ggcaaccatg acgggcaggt cgaacgccag
      481 ggcggacggc gcgacctcca ggaagcccgg cgcaaccagt ccagagatac caagcacccc
      541 gaggatgttg aacacgttgc tgccgatcac gttgccgacc gcaatgtctc gatgacccct
      601 taacgaagcc atgaccgagg tcgcgacctc aggcatcgat gtaccggccg caacgaccgt
      661 gaggccgatg accagctcgc tcacgccgaa ggctcgtgcg aagtcgatcg ctgcattcac
      721 gagcatgtcg gagccgagca caaggaggca caggccgccc agaaccaggg caatctggat
      781 cgtccagtgc ctgtcccagc cagagtcttg ttggacaccc gtttcgtcgt ctgtatccat
      841 ccgggcatgt gcccgagatt gaacgacgag aaacacggtg taggccaaca ggagggctgc
      901 cagcacggcg gcatccagtc tcccaatacg accgtccatg gcgatcacga aaagcagcac
      961 cgatgccagg atcatgacgg gtacttcctg gcgaatgacc tgccgggcga cgatcagcgg
     1021 ggtcacgagc gcggatacgc caagaatgaa cagtacgttg aagatgttgc tgccgacgac
     1081 attgccgagt gcaaggtcac cctggccacg ccacgaagac tgcactgaga ccgccagttc
     1141 cggagagctg gtgccaaagg caacgatcgt caggccaacc actagcggag atacccctgc
     1201 ggcagcggcc aacttcgagg cgcctcgaac gagagtctct gcccccgcca cgagaagcac
     1261 gaggccaagc aagaactgca atagactgga catacgacgc gccaggggcg gactgctctg
     1321 cgggagagag ccccattcta ccagtcgcca tccgccggtc ggcggacaaa ccaaacagcc
     1381 ggtgcgctcg ctatgccacg acaagagata cggcacctgt ctggtagtgg tcctcgacca
     1441 gccaggccgt ctgtacggat cggtgactgc acagccaggg cagggaatgg ccgtaagcgg
     1501 acgataccgg ggcgccgcgc aggacactgg cgccgtggtc agttgcagga cttgtcccgg
     1561 ggtcgccgcc cgccggcccc ggggcgcgcg ccggggctgc caaggccgcc cctgcacggc
     1621 gtcggtcaac cggttcgcta cgacggaaat cgtcgcgtgc actcgaatcg gccgtcgggc
     1681 ccgaagggaa ggttcctttc gtgcacgcca acctccgcct gctcttcggg gaatggatcg
     1741 ccgctgctgt cccgcccgga gtgccggacg cccgtgaggg caatgaagcc tcaggtcgcc
     1801 agtacggctg cagcgaactc cttgtatttg cagccaattt cacctcgctc gaccatcgct
     1861 tgttgtagag gctgaagtac tgtggcacgg taatcgggaa cggtgtgcgg cggcttgcgc
     1921 cgcgtctcat ccggacgggg gagggtgaca acatgattga ctggctggtt cagggcgaga
     1981 gcttcagcaa ttgcaactgc gatcactggt gcccttgtca gttcgagggt ttgcccacgc
     2041 acaattactg tcagggcgtc gaggtcttcc gggtcgatcg gggccacttt ggcgatgtgg
     2101 acttgagcgg ggtcacggtc gccttgttct ttacctggcc cggccccgtc ttcgaaggca
     2161 agggcacgat gcaggcggtg attgatccga gcgccacgcc ggaacagcgc gatgccatct
     2221 cccggatcgt caagggtgcc gagaccaggg agggagccaa tcactggtgg gtgttccatg
     2281 ccatgtcgga taccattctc gaggacctga gcctgccgat cgagttttcg gtggaccggg
     2341 aagcccgcac cgcccgcgta ttcatccccg ggtacctgga agcgactggc gaaccgctca
     2401 ggaatccctt caacggtgcc tcgcaccggg tgcagatccg ctgccccgac ggtatcgagt
     2461 tcgagacggc cgacattggc aacgcttcga gcaccatcac cggccctatc tcgatgacgc
     2521 tgaagaactc ctacggccag ttcacttacc tcgaccagtc tggcaacggt ccggcccacg
     2581 tccagtagcc tgtagtgagt gacgttctcg agcgtgcctt gcggcgcgac cgtgcgatcg
     2641 tcgtcacggc actggcattg gtgacggttc ttgcgtgggc ttatctgctg gccggtgcag
     2701 gcatggggat gagcgccatg gagatgaccc ggcactccat gatgcccatg ccgatgatgc
     2761 agcacgtgca gtgggggttc tcctacgcgg tactgatgtt cttcatgtgg tggatcatga
     2821 tgatcgccat gatgctcccc agcgccacac cggtgatcct gctcgcggca ggaatcaatc
     2881 gccggtctcg cctggaccag gcgccctacg gctcggcagc cgcctttacc acgggatacc
     2941 tgcttgtctg ggcgcttttc agcctgttgg ccacgggtgc ccaatggggt ctcgagacac
     3001 tgggatggct gacgccgatg atggtgagcg ccagcgacct gctggccggc gccatattgg
     3061 tggtcgcggg ggtgtggcaa ttctcaccgt ggaaatccgc gtgcctcagt cactgtcgct
     3121 cgccggtgca gttcctcacc gagcgtcgcc gggcggggaa tatcggcgcc acgatgatgg
     3181 ggatgcatca tggcctgttt tgcctgggat gctgctggtt cctcatgggc ctgttgtttg
     3241 ttggcggcgt gatgaacctc tactggatcg tcgggttggc gatctatgtc gcggctgaga
     3301 aactgctgcc tgggggtccg cgaaccgcca aggtgttcgg cgtgctgctg atcgggtggg
     3361 gcgtcggggt gctcgccggc ctctgaaagt gcttccggca gtcgccgccg aatggcggct
     3421 tcatgaacgg ccgtgcacca cttccgggag acccggctcc cgcggcgctc gagagccgaa
     3481 ccgtcttgtc gtcatgggcg cgctgatggg cgccaggctg cctggcggcg cgtaccgcgc
     3541 gcccgcgccc ccgcagccgg gcccaaatgc tttttttgcg ggcgcgggcg atcctggcat
     3601 cgcccgcgcc cgtgaccccc cggacgtact acggtcgatt ctgcgcgatc cgcgcgacct
     3661 ggcctgattg cgacggcgtc tcgtacgccc ggtagtccgc accgtagttc gtcaggccat
     3721 caggggactg gaacgcccgg ccatggaagt agctgcggag cttgaccatg tccagcgctg
     3781 gcggctcgtc ggagtccggc ccgggtgccg cgtgcgcccc ggctgtcggc aggggcgtgc
     3841 cataggcact gagatcctcg ctgtagtttc ccaggtcgtc ggttgcgtgg aactgcgcgc
     3901 tgtgtaggta cgtgtgcaga tccgtcaggt cggaatactt gcagctgccc gagtcggcca
     3961 tggccgtggt cgccgacagc gccgcgaagg tgcctgcgag cgtcagaaag actgcgatcc
     4021 tactcatgga aatctccttg catgtaattg tccgaccgag ggtccaggcc ggtgcgacag
     4081 ggagtctgct tcttgggccg tgagcggacc gtaatcgaaa cgtaattcgc ggccagaaat
     4141 cgctggcgtt cggtcttggc tgcaatagaa tgacctttgc agcatcatca atggcgagtc
     4201 cgaccgcgcc tggctcggtc ggaccccggg ggcgccgaca gtgcagccat tgcgcatcgc
     4261 cctcctaggg gggttcgagc ttcatgccgc cggcggcgag ccgatcagcc tgccgagccg
     4321 caacgccagg gcgctcctgg cctacctggc gatgtcgaac ggacgagccc aggcccgcga
     4381 caagctcgcg accctgctct ggggtggcaa gagcgcgagt caggctcgtg cgagcctgcg
     4441 gcaggcgctt tcccagctgc gcagaacgct caactccgtc cgcagcgaca tcctgaagat
     4501 cgacggggat gccatcgtgc tcgatccgtc ggcggtagcg gtcgacgcgg ttcgcctgca
     4561 ggaactggtc agcgccggca cgccacaggc cctcgaggag tccgccgctc tctatcacgg
     4621 cgacctcctt gaagggattg gcccgttttc ggaaagcttc
//
LOCUS       DBNHOX010000106         1759 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1037676, whole genome shotgun sequence.
ACCESSION   DBNHOX010000106 DBNHOX010000000
VERSION     DBNHOX010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1037676"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="AC16UZ_02380"
     CDS             complement(<1..274)
                     /locus_tag="AC16UZ_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245555.1"
                     /translation="MTTSARTYTTLRDTISKRAPAPDTGATVEAHTDVIRLIDAMMCM
                     VDQIRLGAQDGTLPFPERARHLAVQMFAYAHDAAQEGGCSGTIDGAI"
     gene            332..1531
                     /locus_tag="AC16UZ_02385"
     CDS             332..1531
                     /locus_tag="AC16UZ_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HIR6245556.1"
                     /translation="MTNDMMNLRDFVEKTPDADLLREMIGFAAERLMELEVGAATGAG
                     YGEKAPLRLVQRNGYRDRDWETRAGTVELRIPKLRRGSYFPGFLEPRRMAEKALTAVI
                     QEAYIQGISTRSVDDLVKAMGMTGISKSQVSRLCTEIDGKVKAFLDRPIEGDWPYLWI
                     DATYLKVRRGGRIVSVAVIIAVGVNADGRREVLGMEVGTSEAEPIWTEFLRKLTRRGL
                     RGVKLVISDAHEGIKAAVTKVLCATWQRCRVHFMRNVLAHAGKSGRRVVSAFIATAFA
                     QETPQAASAQWRAVADQIRPKVPKLATIMDDAEPDVLAYMTFPKEHRAKLHSTNPIER
                     LNGEIKRRTDVVGIFPNDDAIVRLVGALLLEQNDEWAVQRARYMTLETMAPLSDDRPV
                     SLPVAAG"
     gene            complement(1575..>1759)
                     /locus_tag="AC16UZ_02390"
     CDS             complement(1575..>1759)
                     /locus_tag="AC16UZ_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245557.1"
                     /translation="ARLWFTRPSVAGCLIRHGDLTGPKSGRVLRGGSWNNNPRNLRSA
                     NRNRNEPDKIVSRSVV"
BASE COUNT          310 a          553 c          602 g          294 t
ORIGIN      
        1 cgatggcacc gtcgatggtg ccgctgcagc cgccctcctg cgcagcgtca tgggcgtagg
       61 caaacatctg gaccgcgaga tggcgtgccc tttcgggaaa gggcagcgtc ccgtcttgcg
      121 cgccgagcct gatctggtcg accatgcaca tcatcgcgtc aatcaggcgg atgacgtccg
      181 tatgagcctc gaccgtcgcg ccggtgtccg gggcgggagc gcgtttcgag atcgtgtccc
      241 gcagcgtggt gtaggttctg gctgaggtgg tcaccggcga tttgccggta gggtctggtt
      301 gcgacaccaa cctgaaccgg agatttcacc gatgaccaac gacatgatga acctgcggga
      361 cttcgtggag aagaccccgg acgccgatct gctgcgggag atgattggct ttgcggccga
      421 gcggctgatg gagctggagg tgggcgctgc tactggcgct ggctacggcg agaaggcccc
      481 cctgcggctc gtccagcgca atggctaccg ggaccgggat tgggagaccc gggcgggaac
      541 ggtcgaactg cgcatcccga agctgaggag gggcagctat ttccccggct tcctggaacc
      601 gcgccggatg gcggagaagg cgctgacggc ggtgatccag gaggcctaca ttcagggcat
      661 ctcgacgcgc tcggtcgatg acctggtgaa ggccatgggc atgaccggca tctccaagag
      721 ccaagtctca cggctgtgca cggagatcga cggcaaggtg aaggccttcc ttgaccggcc
      781 gatcgagggt gactggccct atctgtggat cgacgccacc tacctcaagg tccggcgcgg
      841 cgggcgcatc gtgtcggttg ccgtcatcat cgccgtcggc gtcaacgcgg acggccggcg
      901 tgaggttctg ggcatggagg tcggcacgtc ggaggccgag ccgatctgga ccgagttcct
      961 tcgaaagctg acgcgacggg gcctgcgcgg cgtgaagctg gtcatctcgg acgcgcacga
     1021 aggcatcaag gcggccgtga cgaaggtact gtgcgcgacg tggcaaagat gccgggtcca
     1081 tttcatgagg aacgtgctgg cgcatgcagg caaaagcggg cggcgcgttg tttctgcctt
     1141 catagccact gcttttgccc aggaaacccc gcaagccgcc agcgcccagt ggcgggccgt
     1201 cgccgaccag atccgcccca aggtgccgaa gctggcgacg atcatggacg atgcggagcc
     1261 cgacgtgctg gcctacatga ccttccccaa ggagcaccgg gccaagctgc actccaccaa
     1321 ccccatcgag cggctgaacg gcgagatcaa gcgccgcacc gatgttgtcg ggatctttcc
     1381 caatgacgat gccatcgtgc gtctcgtcgg cgccctgctg ctggaacaga acgacgaatg
     1441 ggcggtccag cgggcgcgat acatgacgct ggaaaccatg gcacctttga gcgatgatcg
     1501 tcccgtcagc ctgcccgtcg cggccggctg accaggcagg cccgcgccgg aaaccccggt
     1561 gatcccccgg ccacctacac cacgctgcgg gacacgatct tgtccggctc gttcctgttc
     1621 cggttcgccg accgcaggtt cctcgggttg ttgttccagg aaccgccgcg caacacgcgg
     1681 ccggatttgg ggcctgtcag gtccccgtga cggatcaaac agcccgccac ggatggccga
     1741 gtgaaccaca accgggcgg
//
LOCUS       DBNHOX010000107         1624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_780394, whole genome shotgun sequence.
ACCESSION   DBNHOX010000107 DBNHOX010000000
VERSION     DBNHOX010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_780394"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="AC16UZ_02395"
     CDS             <1..513
                     /locus_tag="AC16UZ_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245558.1"
                     /translation="MRFAGRRADANIGNEDGADTDLVARVWVADQKAYYQSKSQERNR
                     GSQLTSALVFATFGVGVTVAIIFAVFQAEIAAPWSNMLVALMGLLPFLAAVRQSYANS
                     TAEREDFVQFGYMYRIFFNADRLLNSARTVDGRRDILRALGEAALEEHGQWMLRQRER
                     PAAGRVLQGG"
     gene            513..>1624
                     /gene="argE"
                     /locus_tag="AC16UZ_02400"
     CDS             513..>1624
                     /gene="argE"
                     /locus_tag="AC16UZ_02400"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF005710.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HIR6245559.1"
                     /translation="MARRFTPREMLEKLIAFPTVSRDSNLELIEFVANYLDDHRVQAR
                     LVASGDGRKANLYATVGPEIEGGVVLSGHTDVVPVDGQNWDSDPFLLHEVDGRLVGRG
                     TCDMKGFLAIALALVPDMGALRYPIHLAFSYDEEVGCLGAPDLIREMTANVPRPKAVI
                     VGEPTSMQVVGAHKSMFIFDTVVRGHEAHSSQQHRGVPAVMVAGRLIGWLAERQRQNA
                     RVADPDSDFVPGYTTLHCGTVRGGTAHNITAAECRFATDIRSLPGEPAESYFREFEQF
                     ARQTLEPEMRAVHPGSGIDFEIRAQVPAFQATGDDPAVALASQLTGQNGLESVPYGAE
                     AGQFQEAGHSVVMCGPGSIDQAHQPNEFLTLEQLQAG"
BASE COUNT          285 a          500 c          549 g          290 t
ORIGIN      
        1 atgagattcg cgggtcgccg ggcggacgcc aacatcggca acgaggacgg ggccgacacc
       61 gacctggtgg cccgggtctg ggtcgcggac cagaaggcgt actatcagag caagtcccag
      121 gagcgcaaca gaggcagtca gttgacctcg gccctcgtct tcgccacatt cggagtcggc
      181 gtgacagtgg cgatcatctt tgcggtcttt caggcggaga ttgccgcgcc ctggtccaac
      241 atgctggtgg ccctgatggg attgttaccc tttctcgctg cggtacggca gagctatgcc
      301 aacagcaccg cggagcgaga ggacttcgtt cagtttggct acatgtaccg gatatttttc
      361 aatgcggacc gcctcttgaa ttctgcccgc acggtcgacg gccgccgcga catcctccgg
      421 gcgctcgggg aggcggcgct cgaagagcat ggtcaatgga tgctccgcca gcgtgagcgg
      481 ccggccgcgg gccgggttct gcagggcggg taatggcgcg acgattcaca ccgcgggaaa
      541 tgctcgaaaa gctgatcgcc tttcccacgg tctcgagaga ctccaacctc gagctgatag
      601 agtttgtcgc caactatctc gacgatcacc gcgtgcaggc cagactcgtc gccagcggcg
      661 atggacgcaa agccaatctg tatgcgacgg tcgggcctga gatcgaaggt ggcgtcgtgc
      721 tgtcagggca tacggacgtg gtgcccgtgg acgggcagaa ctgggactcg gacccgttcc
      781 ttcttcacga ggtcgacggc aggctggtgg gccgcggtac ctgcgacatg aagggttttc
      841 ttgccattgc tcttgctctc gtcccggaca tgggggcgct tcgctatccg attcacctgg
      901 ccttctctta cgacgaggaa gtgggttgcc tgggcgcgcc cgatctcatt cgggagatga
      961 cggccaacgt gcctcgcccc aaggcggtga tcgtggggga gcccaccagc atgcaggtgg
     1021 tgggggccca caagagcatg ttcatcttcg ataccgtcgt tcgtggccac gaggcgcatt
     1081 ccagccagca gcacagaggg gtccccgcgg tcatggttgc cggtcggctg atcggctggc
     1141 tcgccgagcg gcagcgccag aacgcccggg tagcggaccc ggattccgat ttcgtgccgg
     1201 gctacacgac cctgcattgc ggcacggtgc ggggagggac cgcccacaac atcaccgccg
     1261 ccgaatgccg tttcgcgacg gacatccgct cgcttcccgg cgagcccgcc gaatcgtatt
     1321 ttcgggagtt cgagcagttc gcaaggcaga cgctcgagcc ggaaatgcgc gcggtgcatc
     1381 cgggcagcgg gatcgacttc gagatccggg ctcaggtccc agcctttcag gcaacgggcg
     1441 acgacccggc ggtggcactt gccagccagc tgaccgggca gaatgggctg gaaagcgtgc
     1501 cgtacggagc cgaggcgggg cagtttcagg aagccgggca ctcggtggtc atgtgtggcc
     1561 ccggcagcat tgatcaggcc catcaaccca acgagttcct caccctcgag cagctgcagg
     1621 ccgg
//
LOCUS       DBNHOX010000108         4805 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_137790, whole genome shotgun sequence.
ACCESSION   DBNHOX010000108 DBNHOX010000000
VERSION     DBNHOX010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_137790"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1676)
                     /locus_tag="AC16UZ_02405"
     CDS             complement(<1..1676)
                     /locus_tag="AC16UZ_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003119427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HIR6245560.1"
                     /translation="MRPDKMTSRLQNALADAQSMALGKDHAAIEPLHLLAVLVEQKAG
                     SVLPQLVRAGARANELQTRLNERLGQLPTLKSPTGEMSFAPAFVKVLNLADRLAQKAG
                     DDFISTEQVLLALFEDPTVESVLRESGVDKAALEAIVSQARSGETVSDPNAEEHREAL
                     EKYTVDLTAQAEEGKLDPVIGRDDEIRRTVQVLQRRTKNNPVLIGEPGVGKTAIVEGL
                     AQRILNGEVPEGLKNKRILALDMGSLIAGAKFRGEFEERLKAVLNELSKQEGNVILFI
                     DELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQK
                     VLVDEPSVEDTIAILRGLKERYELHHAVDITDPAVVAAARLSHRYITDRQLPDKAIDL
                     IDEAASRIRMEMDSKPEAMDRLERRLIQLKMEAEALKQESDDASRKRLEDLQSSIAEL
                     EREYADLEEIWNAEKASLRGAQDLKEDLDAARGEFEVARRAGDLARMSELQYGTIPEL
                     EKQLAAASNGEAEPRQLLRNSVTEEEVGEVVSKWTGIPISKLLEGEREKL"
     gene            1743..2501
                     /locus_tag="AC16UZ_02410"
     CDS             1743..2501
                     /locus_tag="AC16UZ_02410"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR6245561.1"
                     /translation="MEQRKHPRVQLPLLVELHHPSLPQGKLRCVARDVSKGGVYVHFD
                     QPPTLIPGAKLRLTLLNPSQVESQPTPTVEMMVARTEPTGLGLAFVNATSQHLWASVE
                     RLRDELAIGRDYFQVFQGAVVLNEQDEVLLVQQHGKWGFPGEYLVVGEDAETALKAML
                     TRRFALADLTIDGVLKVGSGATIDLPEAAVFRVYHRVTTSTAEISASAEEAYRKVRWV
                     ENRRSVAQMAFVSKELRELANAALLWAAGRRRAG"
     gene            complement(2527..>4805)
                     /locus_tag="AC16UZ_02415"
     CDS             complement(2527..>4805)
                     /locus_tag="AC16UZ_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HIR6245562.1"
                     /translation="HMRAWLVGLVLGHLVLAAPVAPLPLPVPTAWLMLAGILLGVVRR
                     GLPACGFLVGFAWAAFAMQQLLESRLPGCLDGAEELMVVDVVSEPRRREDGAYTFSAD
                     VRSTRAGLCADVLGRRLRLTWYEAPGIGQGERWRVLARLRTPWAYQNPGGFDFERWLL
                     AEGLNGSGYVVSGTRIGGPRASWRAPWLEGVRGYLETEHPAAAGVLYALVSGDGSLIA
                     SAGWRTLRDTGTVHLMVVSGLHVGLVAGMGYQIARLAVRALPLVLLWVPARRAGALGG
                     VCAASGYVALTGGGVPGLRALCMSALVLGALAWGRRLSPWLVWLVALALVLVGQPAVV
                     HRQGLWLSFGAVAVLIWFFAGRSVREPRLAGVAAALVRAQYALLLGLSPLLVMLQGQI
                     PSSGPVANLVAVPVISFLVIPAALIGALLMELVPFLGEAALGMAAVVVAQLMGVLEAS
                     ASLPPLVAAPRLLPVFVALATGLVMLACGGSGRHLLLCLAWAVWALPPDHGLPRGEFR
                     VLAMDVGQGSAVLVDTKRHRLLFDAGPRYPSGFDLGAQVVLPTLVATGSPRLDALVIS
                     HADIDHSGGKGAVVGAVPVGEVYESRPDSESSRCSAGQSWRWDGVDFRFLHPPPLARA
                     RGLGSNDASCVLLIDNGRERALLPGDIGAAVEGQLLRALDPVDVLLAAHHGSASSSSR
                     AFVRVLEPDVVFISAGRRNRYGHPHPDVVGRFAEVGARVHSTAEHGALVWDSRTPGSV
                     RRWRRDTPRYWVQGSQAD"
BASE COUNT          867 a         1680 c         1508 g          750 t
ORIGIN      
        1 aacagcttct cacgctcgcc ctcgagcaat ttcgatatcg ggatgcccgt ccacttggaa
       61 accacttcgc ccacttcttc ctcggtaacg ctgttgcgca gcagctggcg aggctcggcc
      121 tcgccgttgc tggcagccgc cagctgcttc tcgagctccg ggatcgtgcc gtactgcagc
      181 tccgacatcc tggcaagatc gccggcgcgg cgcgccacct cgaactcgcc tcgtgccgcg
      241 tccagatcct ccttgagatc ctgagcgccc ctcaaggacg ccttttccgc gttccagatc
      301 tcttctagat ccgcgtactc tctctcgagc tcggcaatgg agctctgcag gtcctccagg
      361 cgctttcggc tcgcatcgtc gctttcctgc ttgagcgcct cggcctccat cttcagctgg
      421 atgagccggc gctccagccg gtccatggct tccggcttgg aatccatttc catgcggatg
      481 cggctcgccg cctcgtcgat cagatcgatg gccttgtccg gcagctgacg gtcggtgatg
      541 taccgatgag acaacctggc cgccgcaacg acggccgggt cagtgatgtc gaccgcgtgg
      601 tgaagctcgt aacgttcctt caagcccctg agaatggcga tggtgtcctc cacgctgggc
      661 tcgtcgacga gcactttctg aaagcgtctc tctaacgcgg catccttttc gatgtactgg
      721 cgatactcgt ccagggtcgt cgcgcccacg cagtggagct cgccgcgagc cagggccggc
      781 ttgagcatgt tgcccgcatc catcgcgccc tcggccttac ctgccccgac catggtgtgg
      841 agctcgtcga tgaagaggat gacgttgccc tcctgcttgg agagctcatt gagaaccgcc
      901 ttgagccgct cctcgaactc accccgaaac ttcgctccag cgatgagaga acccatgtcc
      961 agggcgagga tgcgcttgtt cttgagccct tccggcacct caccattgag gatccgctgg
     1021 gccagtccct cgacgatggc cgttttgccc acgccgggct cgccgatgag caccgggttg
     1081 ttcttcgttc gacgctgcag cacctgcacc gtgcggcgaa tctcgtcgtc acgcccgatc
     1141 accgggtcga gcttaccctc ctcggcctgg gccgtaaggt ccaccgtgta cttctccagc
     1201 gcctcgcgat gctcctcggc attgggatcg gagaccgtct ccccgctgcg ggcttgagac
     1261 acgatcgcct cgagcgccgc cttgtccacg cccgattcgc gcagaaccga ttcgaccgtc
     1321 gggtcctcga acagcgcaag gagcacctgc tccgtggaga tgaaatcgtc gcccgctttc
     1381 tgggccaggc gatcggccag gttcaggacc ttcacgaacg ccggggcaaa agacatttcg
     1441 ccggtggggc tcttgagcgt cggcagctgg ccaagccgtt cgttcaaacg cgtctgcagc
     1501 tcgttcgcgc gagcgccggc tcgcaccagc tgcggcagga cggagcccgc tttctgctcg
     1561 acgagaacgg caagcagatg cagcggctcg atcgctgcgt ggtccttacc tagagccatg
     1621 gactgcgcgt ccgctagggc attttgcagt ctgcttgtca ttttgtccgg gcgcatccca
     1681 acgctacctc aacgaaaacc tgctgctaca atgcgtccga ttttcgagat ttcaatggcc
     1741 ccatggagca gagaaagcat ccgagggtcc agcttcccct cctggtggag ctgcaccacc
     1801 cgtcgctgcc ccagggcaag ctccgctgcg ttgcccggga cgtctccaaa gggggcgttt
     1861 acgtccattt cgaccagcct cccaccctca tacccggcgc caagctgcga ctgaccctgc
     1921 tgaaccccag ccaggtggag agccaaccca cgcctaccgt ggagatgatg gtagcgcgga
     1981 ctgagccgac gggtctgggc cttgccttcg tcaatgccac cagtcagcac ctgtgggcta
     2041 gcgtagagcg tctgcgtgac gagctggcga tcggccggga ctacttccag gtgttccagg
     2101 gcgcggtcgt actcaacgag caggatgagg tgttgctcgt acagcagcac ggaaagtggg
     2161 ggtttcccgg cgagtacctg gtcgtcggcg aggacgcgga aacggcgctg aaggcgatgt
     2221 tgacccgccg tttcgctctg gctgacctga cgatcgacgg ggtcctcaag gttggcagcg
     2281 gggctacgat cgacctgccc gaggcagccg tcttcagggt ctatcaccgg gtgaccacct
     2341 cgacggcgga gatctcagcc agcgcagagg aagcgtatcg caaggtccgc tgggtggaaa
     2401 accgacgcag cgttgcccag atggcattcg tcagcaaaga gctgcgcgag ctcgccaacg
     2461 ccgcgttgct ctgggcagcc gggcgccggc gcgccggctg aacaggccaa gggggcgacc
     2521 ggcgcgtcag tccgcttgcg atccctgcac ccagtagcgc ggcgtatccc gccgccaccg
     2581 ccgcaccgag ccaggcgtcc ggctgtccca taccaacgcc ccgtgctccg cggttgagtg
     2641 gacgcgcgcg ccgacctccg caaagcgtcc gaccacatcg ggatgaggat ggccgtagcg
     2701 gttacgtcgg ccggcgctga tgaagaccac gtccggctcc agcacgcgca cgaaagccct
     2761 ggacgacgag gaagcgcttc cgtggtgggc ggccagaagg acgtccaccg gatccagtgc
     2821 tcgcagcagt tgcccttcta cggcggcacc gatgtctcca ggcagcagcg cgcgttcccg
     2881 gccgttgtcg atgagcagca cgcaggatgc gtcattgctc cccaggccgc gagcgcgtgc
     2941 cagcggcggc gggtgcagga agcggaaatc aacgccgtcc caacgccagg actggccagc
     3001 cgagcagcgt gaggattctg agtcgggccg ggattcgtat acctcaccta cgggcacggc
     3061 gcccaccacg gcgcccttgc cgccgctgtg atcgatatcc gcatgactga tcaccagggc
     3121 gtccagccgc ggggagccgg tggcaacgag ggtgggtagg acgacctggg cccccaggtc
     3181 gaagccgctg ggatagcgcg ggcccgcatc gaacagcagc cgatgcctct tcgtgtccac
     3241 cagcactgcg ctgccctgcc ccacgtccat cgcgaggact ctgaactcac ccctgggcaa
     3301 gccatgatca gggggtagcg cccagaccgc ccaggcgagg cacaacaaaa ggtgcctgcc
     3361 cgaaccgcca cacgccagca tgacgagccc ggtagccagg gccacgaaca ccggcaacag
     3421 tcgtggagcg gccacgagcg ggggcagcga cgccgacgct tcgagaacgc ccatcagctg
     3481 cgcgacgacc acggcagcca tcccgagcgc ggcctcgccg aggaacggga ccagctccat
     3541 cagcagcgcg ccgatcagcg ccgccggaat gaccagaaag ctgatgacgg gcaccgcgac
     3601 gaggttggcc accggtcccg atgagggaat ctgtccctgg agcatgacca gcagcgggct
     3661 gagccccagc agcagcgcgt attgagcgcg aacgagagct gcagccaccc cagcgagcct
     3721 cggttcccgg acgctgcggc ctgcgaagaa ccagatcagc accgccaccg cgccgaagga
     3781 gagccagagt ccctgccgat ggactaccgc cggttggccg accagcacca gcgccagggc
     3841 aaccagccat accagccagg gcgacagccg cctgccccac gccagggccc ccagaaccag
     3901 cgcggacatg cacagtgccc gcaatccggg aaccccaccg ccggtcaatg caacgtatcc
     3961 ggatgcggcg caaacaccgc caagggctcc cgcccggcga gccggaaccc acaggaggac
     4021 cagcgggagt gcacgaacgg ctaaacgcgc aatctgatag cccatgccgg ccacgagtcc
     4081 aacgtggagc ccggatacga ccatcagatg aaccgtgccc gtatctcgca gggtgcgcca
     4141 gccggccgag gcgatgagcg agccgtctcc gcttaccagg gcgtagagca caccggcggc
     4201 cgccggatgt tccgtttcga ggtaacctcg cacgccttcg agccacgggg cacgccagga
     4261 tgcgcgcggg ccgccgatcc gcgtgcccga tacgacgtag ccgctcccgt tcagcccttc
     4321 ggcaagcagc cagcgttcga agtcgaaccc tccgggattc tgatacgccc atggcgtgcg
     4381 cagccgcgcc agaacccgcc agcgctcgcc ttgcccgatc ccgggagctt cgtaccaggt
     4441 cagccgaagc cgacggccga ggacgtcggc gcagaggccg gccctggtgc tgcggacgtc
     4501 ggccgaaaaa gtataggccc cgtcctcccg ccggcgcggc tccgagacga catcgacgac
     4561 catgagctcc tcggctccgt ccaggcagcc gggcaggcgg gactccagca gctgctgcat
     4621 cgcaaacgcc gcccaggcga accccaccag aaacccgcag gcgggaagcc cgcgccggac
     4681 gacgcccagc aagattccgg ccagcatgag ccacgccgtc gggacaggca gtggaagcgg
     4741 ggcgacgggc gcggcgagaa cgaggtgacc cagaacgagc ccaacaagcc aagcccgcat
     4801 gtgtg
//
LOCUS       DBNHOX010000109         2082 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_394911, whole genome shotgun sequence.
ACCESSION   DBNHOX010000109 DBNHOX010000000
VERSION     DBNHOX010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_394911"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="AC16UZ_02420"
     CDS             complement(<1..1095)
                     /locus_tag="AC16UZ_02420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6245563.1"
                     /translation="MNFGFTEEQNMLRDQVARFMQEACPIPKVREIGASQTGFDAALW
                     QQAAQLGWLSLIIPEENGGLGLRWIDLTVVLEETARGLCPLPIASQAMTAAAILRCGS
                     DSQKSSWLPAMAAGEVVCTTALYDDPNWIHPDAVQLTATPVEGGVRLDGVKPFVPDAL
                     AADYLLLAYRGPDGLGLAALSRDQVTFTPQPQMDSTKPAGRVVMDAVTVGDEFLLPLS
                     EADLDYLTDVGAVAVTAEMVGAAEAALNMTSEYAKERIQFGKPIGQYQGVKHRLADIY
                     VDVESFKSLLYYAAWAVDEAPDELPRAISMAKGYASDAFARIGIDGIGLHGAIGFTAE
                     YDIQLYLKRSKWARPMYGDSDYHLDRVATLG"
     gene            complement(1092..>2082)
                     /locus_tag="AC16UZ_02425"
     CDS             complement(1092..>2082)
                     /locus_tag="AC16UZ_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6245564.1"
                     /translation="NLITREGCDIVHRLSADADVVLQNFRPGVVERLGIDYARLCADN
                     PGLVYLSISGFGQSGPRAKDRAYDPIIQAYAGITDVQGSIHRDNPDQPEQVNMLLLDK
                     LTAWTGCQAIMAALLARSRTGKGQHVELSMLDTAISFIWSDVAADLILQGDDVDRRPP
                     IRASGTMQTYADGHGFTMTLSQPEFEGLCRAYGLDHIAEDARFASLDLRMRHRAELRE
                     IISSEVDEVALELTVAQAEERLLRHEVPFARVRRLADLPEDEQIQHNQMFRELNHPVA
                     GPLRDARPAPRFASTPAEPGGPAPSVGEHTRQILEEISMGAETEKLFERGIVS"
BASE COUNT          329 a          685 c          704 g          364 t
ORIGIN      
        1 tcccagggtt gcgacccgat ccaggtgata gtcggagtcc ccgtacatgg ggcgcgccca
       61 cttcgaccgt ttgaggtaaa gctgaatgtc gtactcggcg gtgaagccga tggcgccgtg
      121 caggccgatg ccgtcaatgc cgattctggc aaaggcgtcg gaggcgtaac cctttgccat
      181 ggagatggcc cggggcaact cgtcgggcgc ttcgtcgacg gcccaggcgg cgtagtacag
      241 cagcgacttg aacgactcga cgtcgacgta tatgtccgcc agtcggtgct tgacgccctg
      301 atactgcccg attggcttgc cgaactggat gcgctccttg gcgtactcgc tggtcatgtt
      361 cagggcggcc tcggcggcac ccaccatctc cgccgtcacg gcgacggcgc cgacgtccgt
      421 gaggtagtcg agatccgcct cgctcagcgg cagcagaaac tcatcgccga cggtgaccgc
      481 gtccatgacc acacgtccgg ccggcttggt gctgtccatc tgcggctgcg gggtaaaggt
      541 gacctggtcg cggctgagtg ctgccagccc cagcccgtcg ggccctcggt aggccagcag
      601 caggtagtcg gcggcgagag cgtcgggcac gaaaggcttg acgccgtcca ggcgcacgcc
      661 gccctccacg ggcgttgcgg tgagctgcac ggcgtcggga tgaatccagt tcggatcatc
      721 gtacagcgcc gtggtgcaaa cgacttcccc ggccgccatg gctggcagcc agctcgattt
      781 ctgcgagtcg ctgccacatc tgaggatggc ggcggcggtc attgcctggg aggcgatggg
      841 cagcgggcaa agtccccggg ccgtttcctc cagcaccacg gtcagatcga tccagcgcag
      901 tccaaggccg ccgttctcct cggggatgat gaggctcagc cagccgagct gcgccgcctg
      961 ttgccaaaga gccgcatcga agccggtctg tgaagcaccg atctcgcgca ccttgggaat
     1021 ggggcaggcc tcctgcatga agcgcgcgac ctggtcgcgc agcatgttct gctcctcggt
     1081 gaagccaaaa ttcatgaaac gatacctcgc tcgaacagtt tctcggtctc ggcgcccatg
     1141 ctgatttcct ccagaatctg tcgcgtgtgc tctcccacgc tcggcgcggg cccgccgggc
     1201 tccgccgggg tgctggcgaa tcgcggggcg ggcctcgcgt cgcgcaatgg ccccgccacg
     1261 gggtgattca gctcccggaa catctggttg tgctggatct gctcgtcttc gggcaggtcg
     1321 gccaggcgcc gcacccgggc gaaaggaacc tcgtgacgca gcagcctctc ctccgcctgc
     1381 gccaccgtga gctccagcgc cacctcgtcg acctcggagc tgatgatctc gcgcagctcg
     1441 gcgcggtgcc gcatgcgcag gtccaggctg gcgaagcggg cgtcctcggc gatgtggtcc
     1501 aggccgtagg cacggcacag gccctcgaac tccggctggc tcagcgtcat cgtgaagccg
     1561 tggccgtcgg cgtaggtctg catggtgccg gaagcgcgga tgggcgggcg cctgtcgacg
     1621 tcgtccccct gcagaatcag gtcggcggcc acgtcggacc agatgaacga gatggcggtg
     1681 tccagcatgg acagctccac gtgctggccc ttgcccgtgc ggctgcgtgc cagcagagcg
     1741 gccatgatcg cctggcaccc ggtccaggcc gtcagcttgt ccagcagcag catgttcacc
     1801 tgctcgggct ggtcgggatt gtcccgatga atgctcccct gcacgtcggt gatgccggcg
     1861 taggcctgga taatggggtc gtaggcccga tctttggcgc ggggcccgga ctggccgaac
     1921 ccgctgatgg aaaggtagac gagccccgga ttgtcagcac aaagcctcgc gtaatcgatg
     1981 cccaggcgct ccaccacacc gggcctgaag ttctgcagaa ccacgtcggc gtccgccgag
     2041 aggcggtgga cgatgtcgca gccctcccgg gtgatgagat tc
//
LOCUS       DBNHOX010000110         3578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1616097, whole genome shotgun sequence.
ACCESSION   DBNHOX010000110 DBNHOX010000000
VERSION     DBNHOX010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1616097"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..557
                     /locus_tag="AC16UZ_02430"
     CDS             258..557
                     /locus_tag="AC16UZ_02430"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6245565.1"
                     /translation="MKLDAASACLESLGNSTRLAIYRALVRAGPAGLPVGKLQARLDI
                     PGSTLSHHIAHLVKNDLVSQAREGRVLRCTANYRTMNDLITYLSDECCRDTPRTT"
     gene            557..1219
                     /locus_tag="AC16UZ_02435"
     CDS             557..1219
                     /locus_tag="AC16UZ_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012455.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HIR6245566.1"
                     /translation="MARRLAGEFMGTLLLLATVVGSGIMGEQLSGGNAAIALLGNTIA
                     TGAILVVIILMFAPVSGAHFNPAVTLTFLLRREIPAGAAAAYCVTQVAAGLAGVWLAH
                     GMFGLEIVQTSENSRSGFGQWLAEGVATFGLVATILAVLRSRPDLVAFAVGLYISAGY
                     WFTASTSFANPAVTIARAFTDTFSGIQPDHVPAFIAAQLVGAALATWTFGWLLSEGER
                     TN"
     gene            complement(1216..1866)
                     /locus_tag="AC16UZ_02440"
     CDS             complement(1216..1866)
                     /locus_tag="AC16UZ_02440"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF036807.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6245567.1"
                     /translation="MTLKVIGAGFGRTGTLSLKLALERLGLEKCYHMLEVMQNEGHAA
                     VWRAAARGESVGWDGLFAGYQASVDWPSCNFWEAQLQHFADAKVILTRRDPERWYASV
                     MNTIYPSSVARREQADPAIREHMDMVFEVIWDGLFDGRMDDKEHVIAIYEAHGQHVID
                     ALPPEKLLVFEASAGWGPLCDFLDLPVPDEPYPWVNSTEDFRSRVAAASSKPQRDG"
     gene            complement(1889..2326)
                     /locus_tag="AC16UZ_02445"
     CDS             complement(1889..2326)
                     /locus_tag="AC16UZ_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HIR6245568.1"
                     /translation="MNGLRSIALALALVTSAAVLADGIRSPAPEGASLYFIAPADGAV
                     VSGPFTVKFGLKGMGVAPAGVDAPNTGHHHLLINLEEAVNPEVPLPATDQVRHFGGGQ
                     TETQLSLPAGTYRLQLMLGNYLHVPHDPPVMSELITVTVADQP"
     gene            complement(2348..2845)
                     /locus_tag="AC16UZ_02450"
     CDS             complement(2348..2845)
                     /locus_tag="AC16UZ_02450"
                     /inference="COORDINATES: protein motif:HMM:NF016001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR6245569.1"
                     /translation="MTIRDAVWRGFSLFVSLKPVTVFFMNLATWVDRPLMRATSGRIR
                     LSFVIPTLLLRCTGARSGLLREVPLLYVPEGDGALLVGSNGGKPYDPAWCHNLRAQRR
                     VSCLLAGREGTFRVEELSGADRDAAWESAVAIYPGYRRYEQRAGRVIPLFRLIPEADG
                     RARQT"
     gene            complement(2842..>3578)
                     /locus_tag="AC16UZ_02455"
     CDS             complement(2842..>3578)
                     /locus_tag="AC16UZ_02455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6245570.1"
                     /translation="SGDYGPARVCAGIVGYVDLTLGSSAGPVLDAHMAASDRFKGIRH
                     AAGWDPSEKVRNSHTNPSEGLLLDDEFRAGFAELGKRGLSFDAWLYHPQIPQLTDLAR
                     AFPHTTIIFDHFGGPLGIGPYEGRQDQIFAAWQRDVAALAECHNVYAKLGGLVMPVNG
                     FGFHKRERPATSDEIVAATGRYYRHAIDCFGVDRCMFESNFPVDKQSCSYHVLWNAFK
                     KMVADGSPAEKASLFHDTAARAYRLT"
BASE COUNT          629 a         1193 c         1119 g          637 t
ORIGIN      
        1 tcacgaagtt ggcatagccc ggctcccgct tgttggccgc cacgcccagg atccgcgagt
       61 agtagctcac cgcctcgtct atgtctgcga cgttcagtgc cagttgaaga cgcatgcgat
      121 tctcctgcat caatactttg aaaatatatt tccactttat tcgaaataat tgacccgcgc
      181 caataccgtt tcgttatttc tagcctcatg gaaataacgt aaactgcgat ggcccgaagg
      241 attcaagcag actccagatg aaactagatg ccgcctcggc atgcctcgaa agcctcggca
      301 acagcacccg gctcgccatc tacagagccc tcgtgcgtgc cggaccggcc ggcttgcccg
      361 tcggaaagct tcaggctcgt ctggacattc ccgggtccac cctttcgcat cacatcgctc
      421 acctggtgaa aaacgatctg gtctcccagg cccgcgaggg ccgcgtgctt cgctgcacgg
      481 cgaactacag gaccatgaac gatctgatca cctacctgtc cgacgagtgc tgtcgggaca
      541 ccccgaggac cacctgatgg ctcgccggct tgcaggcgag ttcatgggca ccctgctgct
      601 gctggccacc gtcgtgggat cggggatcat gggagagcag ctttccgggg gcaacgcggc
      661 catcgccctg ctcggcaaca ccatcgccac cggcgccatc ctggtggtca tcattttgat
      721 gttcgcgccc gtctccggag cccacttcaa ccccgcggtc acgctcacgt tcctcctgcg
      781 gcgcgagatc cccgcgggtg cggcggccgc ctactgcgtg acccaggtgg ccgctgggct
      841 ggccggcgtg tggctggccc atgggatgtt cgggctggaa atcgttcaga catccgagaa
      901 ttcccggtca ggcttcgggc aatggctggc cgagggggtc gccaccttcg gcctggtagc
      961 gaccatactc gccgtgctgc gctcccgtcc cgacctggta gcgttcgccg tggggctcta
     1021 catctccgcc ggctactggt tcaccgcgtc gacgtccttc gccaaccccg cagtgaccat
     1081 cgcccgggcg ttcacggaca cgttttcggg aattcagccc gaccacgtcc ccgcattcat
     1141 cgcagcccag ctcgtcggag ccgcgctcgc aacctggacg ttcggctggc tgctctccga
     1201 gggcgaaagg accaactaac cgtcccgctg aggcttcgag cttgctgcgg ccacccgcga
     1261 gcgaaagtcc tcggtgctgt tcacccaggg atatggctcg tcgggcaccg gcagatcaag
     1321 aaaatcgcac aacggtcccc aaccggcact cgcctcgaag acgaggagct tttccggcgg
     1381 cagcgcatcg atgacgtgct ggccgtgggc ttcgtagatc gcgatgacgt gctctttgtc
     1441 atccatgcgc ccgtcgaaca gaccatccca gatcacctcg aacaccatgt ccatgtgctc
     1501 gcggatcgcc ggatccgcct gctcgcgccg ggcaacgctc gacggataga tcgtgttcat
     1561 gacgctggcg taccagcgct cggggtcccg gcgggtgagg atgaccttgg cgtcggcaaa
     1621 gtgctgaagc tgcgcttccc agaagttgca ggagggccag tccaccgaag cctggtagcc
     1681 ggcaaagagg ccgtcccagc ccaccgactc gccccgtgcc gccgcccgcc agacggccgc
     1741 gtgaccctcg ttctgcatga cctcgagcat gtggtaacac ttctccagcc ccaggcgttc
     1801 cagggcgagc ttgagcgaga gcgtgcccgt tcggccgaag cccgcgccga tgactttcag
     1861 cgtcacgatg agttcctcga ccttgtcctc acggctggtc ggcgaccgtg accgtgatca
     1921 actcggacat caccggggga tcgtgaggca cgtgcagata attgcccagc atgagctgca
     1981 ggcggtaggt tccggccgga aggctgagct gtgtttccgt ctggccgccg ccgaagtggc
     2041 gaacctgatc cgtggcaggc agcggcactt cgggattcac cgcttcctcg agattgatca
     2101 ggaggtggtg gtggccggtg ttgggcgcgt ccacgccggc cggggcgacg cccatgcctt
     2161 tcaggccgaa cttcaccgtg aacggcccac tcaccacggc gccatcggcg ggggctatga
     2221 aatagagcga cgccccttct ggtgccggcg agcggatgcc gtccgccagc acggccgccg
     2281 acgtcaccag agccagcgcc aaggcaattg atctcaagcc gttcatcgtg atcctccgtc
     2341 gatgttttca ggtttgccgt gcccgcccgt cggcctccgg gatcaggcgg aacaacggga
     2401 tgacccgtcc tgcccgctgc tcgtagcgtc ggtatcccgg gtagatggcg accgccgact
     2461 cccaggccgc gtctcgatcc gccccgctga gctcctccac ccggaaggtg ccctcgcggc
     2521 cggcgagcag gcagctgacc cggcgctggg cacggagatt gtgacaccag gccggatcat
     2581 acggctttcc gccgttggaa cccacgagca gcgccccgtc gccctcggga acgtagagca
     2641 gcggcacctc cctgagcagg ccgctgcgcg cccccgtgca ccgcaacagc aacgtcggga
     2701 tcacgaagct gaggcggatg cgtccggaag ttgcccgcat gaggggtctg tcgacccagg
     2761 tggcgagatt catgaagaat acggtcaccg gcttgagcga cacgaagagg gaaaatcccc
     2821 gccagacggc gtcccgaatg gtcacgtcag gcgataggct cgagcggccg tgtcgtgaaa
     2881 gagcgaagcc ttctccgccg gagacccatc ggccaccatc ttcttgaagg cattccagag
     2941 cacgtgatag gagcagctct gcttgtccac cgggaagttg ctttcgaaca tgcacctgtc
     3001 cacgccgaag cagtcgatgg catgccggta gtagcggccg gtcgccgcca cgatctcatc
     3061 ggaggtagcg ggccgttcac gcttgtgaaa cccgaagccg ttgacgggca tgaccaggcc
     3121 accgagcttg gcgtagacgt tgtgacactc ggccagcgca gccacgtcgc gctgccaggc
     3181 ggcgaaaatc tggtcctgcc ggccttcgta ggggccgatg ccgaggggcc cgccgaagtg
     3241 atcgaagatg atcgtcgtgt gcgggaaagc tcgcgccagg tcggtgagct gggggatctg
     3301 tgggtggtag agccaggcgt cgaagctcaa tccgcgcttg ccgagctccg caaaacccgc
     3361 ccggaattcg tcgtcgagca acaaaccctc ggacgggttg gtgtgggagt tgcgaacctt
     3421 ctcgctggga tcccagccgg cggcgtgacg aatccccttg aaccggtcgc tcgccgccat
     3481 gtgggcgtcg agcacggggc ccgcggagga tccgagggtc aggtctacgt agccgacgat
     3541 accggcacac acccgggcgg gcccgtaatc gccgcttg
//
LOCUS       DBNHOX010000111         2259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_780560, whole genome shotgun sequence.
ACCESSION   DBNHOX010000111 DBNHOX010000000
VERSION     DBNHOX010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_780560"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="AC16UZ_02460"
     CDS             complement(<1..388)
                     /locus_tag="AC16UZ_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015060.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HIR6245571.1"
                     /translation="MFETLRMDRQMPPSRSRQVLGLIGWLLVSFAAAAIGAFASASAG
                     EFYRELVRPSWAPPGWLFGPVWSVLYACMGIAAWLVWRARGFSRARSALLLFIAQLAA
                     NALWTWLYFAWRLGSLAFAEILLLWGL"
     gene            complement(497..1270)
                     /locus_tag="AC16UZ_02465"
     CDS             complement(497..1270)
                     /locus_tag="AC16UZ_02465"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6245572.1"
                     /translation="MKPFEGRVALVTGGSSGIGEATALNFARGGASVVIAARRPEQSR
                     SVIERISKLGARGHFVRTDVTVAADVEAMVAAAVAQFGRLDYAVNNAGIAGRKCTPVA
                     DVTESEWDQLMNVNLKGVWMCMKQEIPAMLASGGGAIVNITSIYGLKPSDAGHAPYSA
                     SKFGVIGLTKSAAVDYGQMGIRVNAVAPGFTRSEMVDPERPGSVEQYQALKSRHSAMD
                     RFGEAAETANAITWLCSDGASFVNGAVLPVDGGDTTRLY"
     gene            complement(1360..1800)
                     /locus_tag="AC16UZ_02470"
     CDS             complement(1360..1800)
                     /locus_tag="AC16UZ_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HIR6245573.1"
                     /translation="MRIWTLHPRYLDPQGLVALWREALLARAVLRGETTGYRQHPQLQ
                     RFQSHAAPRYAINAYLSSVLEEARSRGYAFNARKVGPVRAIATIECSAGQLHYEWRHL
                     MGKLRTRSPSLYRTWRGVTAPEPHPLFRIRPGPVEPWERPRGRD"
     gene            complement(1891..>2259)
                     /locus_tag="AC16UZ_02475"
     CDS             complement(1891..>2259)
                     /locus_tag="AC16UZ_02475"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6245574.1"
                     /translation="AFALNALIESDHVQLLHGEVEVVSTPSNSGKGQKISRCPKCHVA
                     VWSNYAGAGDAIRFIRVGTLDEPDRLPPDIHIYTASKQPWVVIPPGSASVPGYYRDSE
                     RWPKGSLERRAALLARMQQR"
BASE COUNT          387 a          765 c          736 g          371 t
ORIGIN      
        1 tcaggcccca tagcaggagg atctcggcga acgccaggct gcccaggcgc cacgcgaagt
       61 acagccaggt ccatagtgca ttggcggcga gttgggcgat gaacaacagc agtgcgcttc
      121 gggctcgcga gaagccgcgc gcgcgccaga ccagccaggc ggcgatgccc atgcaggcgt
      181 acagtaccga ccagaccgga ccgaacagcc agccaggcgg cgcccaggac gggcggacca
      241 gctcacggta gaactcgcca gcgctcgccg aggcgaacgc gccaatcgcc gctgcggcaa
      301 acgaaaccag cagccagcct atcaggccca ggacctggcg ggatctcgat ggcggcattt
      361 ggcggtccat ccgaagcgtc tcgaacaacg cgctcacctc caggtcacag cagagcctac
      421 gcgcctactg gaagcccggc cgggtcgcgg cgcgggccct ttccgtctgg cgcttgaccc
      481 aagccggacc ccccgcctag taaagacgcg ttgtgtcgcc accgtcaacg gggaggaccg
      541 ctccgttgac gaagctggcg ccgtctgagc acagccacgt aatcgcgttg gcggtttccg
      601 cggcctcgcc aaagcggtcc atggccgagt ggcgggactt gagcgcctgg tactgctcga
      661 cactgcccgg ccgctcggga tccaccatct cggaccgcgt gaacccgggt gcgacggcgt
      721 tgacgcggat gcccatctgg ccgtagtcca cagcagcgga cttggtgagg ccgatgacgc
      781 cgaacttgct cgcactgtac ggggcgtgcc cggcgtcgct tggcttgagc ccgtagatcg
      841 aggtgatgtt gacgatggcg ccgccgccgc tggccagcat cgcgggtatc tcctgcttca
      901 tgcacatcca tacccccttc aggttgacgt tcatgagctg atcccactcg ctctccgtca
      961 cgtccgccac cggagtgcac ttgcggccgg cgatgcccgc gttgttgact gcgtagtcca
     1021 ggcggccgaa ttgcgcaacg gcagccgcaa ccatggcctc gacgtcagcg gccaccgtga
     1081 cgtcagtgcg gacgaagtgc ccccgggccc ccagcttcga gatccgctcg atcacggacc
     1141 ggctctgctc agggcgtcgc gcggcgatga cgacgctcgc cccgccgcgg gcgaagttca
     1201 acgccgtggc ttcgcctatg cccgagctcc cccctgtcac cagggcgacc ctgccttcga
     1261 atggtttcat cgcggacctc cgaatcggca agcgcattct ggcatcagga atcgccagcg
     1321 cgccatttgt gacgccgagc gccacgtccc gcccgatggt cagtcgcgac cgcgcggacg
     1381 ctcccacggt tcaaccggtc cgggcctgat gcggaacaag gggtgcggtt ccggcgcggt
     1441 gacaccgcgc catgtgcgat agagagacgg actccgggtc ctcagcttgc ccatcaggtg
     1501 ccgccactca tagtggagct ggccagcgct gcactcaatc gtggcgatgg cccgcactgg
     1561 tccgaccttt ctggcgttga aagcgtatcc gcgggaccgt gcctcctcga gcacggaact
     1621 caggtaggcg ttgatcgcgt acctcggagc ggcgtgcgac tggaaccgct ggagttgagg
     1681 gtgctggcga tagcccgtgg tctccccgcg cagcacggca cgggccagga gcgcttcacg
     1741 ccacaaggcg accaggccct ggggatccag atatcgtggg tggagagtcc agatgcgcat
     1801 atgccgccgg tggccgctag cccgggcaaa ccccgaagaa agcctcccgc cgatgcgggt
     1861 tgggtccgct tgcgcagtta cgcattgtgg ttacctctgt tgcatgcggg ccagcaacgc
     1921 ggcacggcgt tccaggctcc ccttcggcca cctctcggaa tcccggtagt acccgggcac
     1981 ggaggcggac cctggcggaa tgacgaccca tggctgcttc gacgccgtgt agatgtggat
     2041 gtctggtggc aggcggtcgg gttcatccag tgtgcctaca cggatgaaac ggattgcatc
     2101 cccggcgccc gcatagttgc tccaaaccgc gacatggcac ttcggacagc gagagatctt
     2161 ctggcccttg ccactgttgg acggggtgct cacgacttcg acttccccgt gcagcagttg
     2221 cacatggtct gattcgatga gcgcattcag ggcgaaggc
//
LOCUS       DBNHOX010000112         1973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_588163, whole genome shotgun sequence.
ACCESSION   DBNHOX010000112 DBNHOX010000000
VERSION     DBNHOX010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_588163"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="AC16UZ_02480"
     CDS             complement(<1..827)
                     /locus_tag="AC16UZ_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245575.1"
                     /translation="MTGNSSILSDLPATAGVARSTRREGVDVSLNAPTPAQPAIASPE
                     WAVAGWIIRFVRLMAGHRLAIPQEPGDVFLKVVAGALTDRGAFARPREIRDTLLLSEE
                     VTAGHDGALVAVMTRGDGAPERVEVMSELTLHGPLEDAFRWQRFDEKFGHVTHAFDGV
                     EAYMSPGFHLLEEDGDEVCYVNLWTSGKGVDLTTHNHANDPSPAAPAFAETHWVFYNG
                     SGRGGMYGCDEPGAPRTRYPMQTGQEHGPFFAVDEHGAPRRRENGAVAYPWHGWESG"
     gene            complement(824..>1973)
                     /locus_tag="AC16UZ_02485"
     CDS             complement(824..>1973)
                     /locus_tag="AC16UZ_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6245576.1"
                     /translation="GWTSYEKTIEACPFTPVADETVGDSMLYSSGTTGRPKGIKRPLT
                     GQSPAKGIPGVEQMSPYGFSQESVYLSPAPLYHAAPFAFCMRTLSLGGTVIMMERFDP
                     LEALGCIERYRVTHSQWVPTMFVRLLNLDDEARTGFDLSSHRCAVHAAAPCPVPIKQQ
                     MMTWWGPIIWEYYAGTERNGSTLIDPHDWLAHPGSVGRARSGVIHICDDDGRELDPGQ
                     EGLIYFEQPERPFEYHNAPDKTANATHPDHGNWTALGDVGYLDDEGYLYLTDRKAFVI
                     ISGGVNVYPREIEDVLIVHPKVADVAVFGVPNEEFGEEVKAVVQPAPGTSPGDALAAE
                     LVGHARAHLAGFKVPRSIDFIDEMPRLPTGKLYKQPLRARYWPERKST"
BASE COUNT          299 a          648 c          675 g          351 t
ORIGIN      
        1 tctccggact cccacccgtg ccagggatac gccacggcgc cgttctcgcg gcggcgtggc
       61 gcgccgtgct cgtccaccgc aaagaacggg ccgtgctcct gaccggtctg catggggtag
      121 cgcgtacggg gcgcgccggg ctcgtcgcag ccgtacatgc ccccgcgtcc ggaaccgttg
      181 tagaacaccc aatgggtctc ggcgaaggcg ggggcggcgg gggacggatc gttggcgtga
      241 ttgtgggtgg tcagatcgac gcccttgccc gaagtccaca ggttgacgta gcacacctcg
      301 tcgccatctt cctcaagcag gtgaaatccg ggcgacatgt acgcctcgac gccgtcgaac
      361 gcgtgcgtga cgtggccgaa tttctcgtcg aatcgctgcc agcgaaaagc gtcctcgaga
      421 gggccgtgca gggtcagctc ggacatgacc tcgacccgct ccggcgcgcc gtcccctctc
      481 gtcatcacgg caaccagcgc gccgtcgtgg cccgccgtca cttcttcact gagtaacagg
      541 gtgtcgcgga tctcgcgcgg acgggcgaaa gcgccgcgat cggtgagcgc tccggcgacc
      601 accttgagga aaacgtcacc cggttcctgt gggatggcca ggcggtggcc ggccatcagg
      661 cggacgaacc tgatgatcca gcccgccacc gcccattccg gcgatgcgat ggccggctgg
      721 gcgggcgtgg gcgcattcag gctgacgtcc accccttctc tgcgggtgct gcgggcgact
      781 cccgcggtgg caggcaggtc ggataaaatg ctcgagtttc cggtcatgtc gatttccttt
      841 ctggccagta acgtgcccgc agcggctgct tgtagagctt accagtgggc agcctcggca
      901 tctcgtcgat gaagtcgatg gagcggggca ccttgaaacc ggcgaggtgg gcgcgcgcat
      961 ggccgacgag ctccgccgcc agggcgtcgc cggggctcgt gccgggcgcc ggctgcacca
     1021 cggctttgac ctcctcgccg aactcctcgt tcggcacccc gaacacggcc acgtccgcca
     1081 ccttcgggtg aacgatgagc acgtcctcga tctcgcgcgg gtagacgttc acccctccgg
     1141 agatgatcac aaacgccttg cggtcggtca ggtagaggta accctcgtcg tccagatagc
     1201 cgacgtcgcc gagggccgtc cagttgccgt ggtcggggtg ggtggcgttg gccgtcttgt
     1261 cgggcgcgtt gtggtactcg aacggccgct cgggctgctc gaagtagatc aggccttcct
     1321 ggcccgggtc gagctcccgg ccgtcgtcgt cacagatgtg gatgacgccg ctgcgcgccc
     1381 gtcctaccga gccgggatgg gccagccagt cgtgggggtc gatcagggtc gagccgttgc
     1441 gctccgtgcc cgcgtagtat tcccagatga tcggtcccca ccacgtcatc atctgctgct
     1501 tgatggggac cgggcagggt gccgcggcgt ggacggcaca acggtggctg ctgaggtcga
     1561 atcccgttcg cgcctcgtcg tcgaggttca gcaaacgaac gaacatggtc ggcacccact
     1621 ggctgtgggt aacccggtat cgctcgatgc agccgagggc ctccagcgga tcgaatcgct
     1681 ccatcatgat caccgtgccg ccaaggctca gcgtccgcat gcagaatgcg aacggcgcgg
     1741 cgtggtagag gggcgcaggt gacaggtaga cgctctcctg gctgaacccg taggggctca
     1801 tctgctccac cccgggtatg cctttcgccg gcgattggcc ggtcaggggc cgcttgatgc
     1861 ccttgggccg gcccgtggtg cccgagctgt agagcatgga atcgcccacg gtctcgtcgg
     1921 caaccggggt gaaggggcag gcctcgattg ttttctcgta cgacgtccat ccc
//
LOCUS       DBNHOX010000113         2901 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1102132, whole genome shotgun sequence.
ACCESSION   DBNHOX010000113 DBNHOX010000000
VERSION     DBNHOX010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1102132"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1193
                     /locus_tag="AC16UZ_02490"
     CDS             168..1193
                     /locus_tag="AC16UZ_02490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6245577.1"
                     /translation="MADVNRQVVLASRPDGYPVDSDFRVEEAGVPDVGEGQFLLRNAY
                     VSLDAGFRNWMNEDSGDDVLPAMALNAPVMGLVLGQVVESRHSEYPVGEWLMARLAWE
                     EYSISDGSDFISRLPEPRECPLPHYLGILGDTGLSAYFGLLDIGRPQPGETVLVSAAG
                     GAVGNVVGQLAKIHGAKAVGLAGTDEKCARLVEELGFDAAVNHRSPDLAGAIAAACPD
                     GVDVYFDSVGGPLLETVLWQIANGARIVMCGAVAAYNALEPIPGPVNLFQLVTHQALM
                     QGYLTHLAEHRYPEARAAMTRWLKSGELKNVEYRLQGIENVGRAFCDLFGGNNFGKTI
                     VRLRDEQ"
     gene            1231..1467
                     /locus_tag="AC16UZ_02495"
     CDS             1231..1467
                     /locus_tag="AC16UZ_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245578.1"
                     /translation="MPILKHPLSGAIYTARDDGLVEVDNNGLKGLFHFDGRHHSGELR
                     HADPHMLLWLAGPQLPADAAVRINRPAPVPPSEG"
     gene            1524..2861
                     /locus_tag="AC16UZ_02500"
     CDS             1524..2861
                     /locus_tag="AC16UZ_02500"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6245579.1"
                     /translation="MAAIVDRSKGISYQELIAQDPVAPPKTLTLENAYQPGIATRVPV
                     SRYASKEFHDLEMEKLWPRVWQMACREEEIPEVGNYTIYEIGSFSIVVVRTNQGIKAH
                     HNVCRHRGRRLCDHPGHASSFICPFHGFSWNIDGSLRSVTSEWDFPHIDKDELTLTPV
                     RCETWAGWVFVNMDMNAEPLMDFLGDLPAHFAVWEPENRYIEAHVAKIMSCNWKACQE
                     AFMEAFHVINTHPQILVGIGDENTQYDVWGNFSRAITPNGTPSPHLRFEPDEQELFDS
                     VTMRYMDRPSLGEVPEGMTARAMMAAGARPAIAALVGEGKDLSDASLSDSIYYTLFPN
                     FHPWGGYNRIVYRFRPYKNHHDKSIMECYYLSPCAGERPEPAPVHWLGEDEDWTEATE
                     LGILAKVFQQDTFNLPKVQIGLEAAQYEEIVLANYQESKLRHFHTLLEEWIGR"
BASE COUNT          541 a          934 c          935 g          491 t
ORIGIN      
        1 gggcactacg atcagagttt cttgagagcc cagctgtccg ctttccagcg cagctacgct
       61 tacgtgctgg cccacggtct cgatgcggtg aacgcagaca tgcagaactt cgcgcacctc
      121 aaagagaact tcgtggccgc ggccacctct catcgctact tcgcctgatg gccgacgtca
      181 atcgacaggt cgtgctggcg tcgcggcccg acggctatcc ggtcgacagt gacttccgcg
      241 tggaggaggc aggggtaccc gacgtcgggg aagggcagtt tcttctgcgc aatgcctacg
      301 tctccctgga tgcgggtttc cgcaactgga tgaacgagga cagcggcgac gacgtcctcc
      361 cggccatggc gctgaacgcg cccgtcatgg ggctcgtcct ggggcaggtg gtcgagtcac
      421 ggcactccga gtatccggta ggcgagtggc tcatggccag gctggcctgg gaggagtact
      481 cgatcagcga cggctccgac ttcatttcgc ggctgccgga acctcgcgag tgcccgctgc
      541 cgcattacct ggggattctg ggtgatacgg ggctctcggc ctatttcggc ttgctggaca
      601 tcggccggcc gcagcccggt gagacggtgc tggtgagcgc cgcgggcgga gccgtcggca
      661 acgtggtggg gcagctcgcg aagattcacg gggccaaagc cgtgggcctg gccggtaccg
      721 acgaaaagtg cgcgcgtctc gtcgaagagc tcggcttcga cgcggcggtc aatcaccgaa
      781 gcccggatct ggccggtgcc atcgcggcgg cctgtcccga cggtgtcgac gtctacttcg
      841 acagcgtcgg cggcccgctg ctggagactg tgctctggca gatcgccaac ggcgccagga
      901 tcgtcatgtg cggcgcggtg gccgcctaca acgccctgga gccgataccg gggccggtca
      961 acctctttca gctggtgacc catcaggcgc tcatgcaggg ctacctcacg cacctcgccg
     1021 agcatcgcta tccggaagcc cgggccgcca tgacgaggtg gctgaagtcg ggcgagctca
     1081 agaacgtcga gtaccggctt cagggcatcg agaacgtcgg ccgcgcgttc tgcgatctgt
     1141 tcggggggaa caatttcggc aagaccatcg tgcggttgcg cgacgagcag tgatgcggta
     1201 cctgtatacg cagaggagga gaagaagacc atgccgattc tgaagcatcc cctgtcggga
     1261 gccatttaca cggcccggga cgacgggctc gtggaggtgg acaacaacgg gctcaaaggc
     1321 ctgtttcact tcgacggtcg ccaccattcc ggcgagctgc gccacgccga tccgcacatg
     1381 ctgctgtggc tggccgggcc ccagctgccg gctgacgcgg ccgtgcgcat caaccgcccc
     1441 gcaccggtgc cgccctccga gggctgaacc ggcgcaccgg gcgcggacga caccgaacca
     1501 accgacatat ccaggagaac gaaatggccg ccatcgtcga ccgatcgaaa ggcatcagct
     1561 accaggagct catcgcgcag gatcccgtgg cgccgccgaa gaccctgacc ctcgagaacg
     1621 cctaccagcc gggcatcgcg acccgcgtcc ccgtgagccg ctacgcatcg aaggaatttc
     1681 acgatctgga gatggagaag ctgtggcccc gggtctggca gatggcctgc cgtgaggagg
     1741 agatccccga ggtcggtaac tacaccatct acgagatcgg cagcttctcc atcgtggtgg
     1801 tccgtaccaa ccagggcatc aaggcacatc acaacgtctg ccgtcaccgg ggccgacggc
     1861 tttgtgatca tccggggcac gcctcgagct tcatctgtcc cttccacggg ttcagctgga
     1921 acatcgacgg atcgctgcga tcggtcacct ccgagtggga tttcccccac atagacaagg
     1981 acgagctgac actcacgccc gtgcgttgcg agacctgggc cggctgggtg ttcgtcaaca
     2041 tggacatgaa cgccgagccg ctcatggact tccttggcga tctgcccgcg cacttcgccg
     2101 tctgggagcc ggagaaccgg tacatcgaag cccatgtggc caaaatcatg agctgcaact
     2161 ggaaagcgtg tcaggaagcc ttcatggagg ccttccacgt catcaatacc cacccgcaga
     2221 ttctggtggg gatcggcgac gagaacaccc agtacgacgt ctggggaaac ttctcccggg
     2281 ccatcacgcc caacgggacg cccagcccgc acttgaggtt cgagccggac gagcaggagc
     2341 ttttcgactc ggtgaccatg cgttacatgg atcggccctc cctgggggag gtccccgagg
     2401 gaatgaccgc ccgggccatg atggcggccg gtgcgcgccc cgccatagcg gccctcgtgg
     2461 gggagggtaa ggacctgtcg gacgcttcgc tgtcggacag catctactac acgctcttcc
     2521 ccaactttca cccgtggggc ggctacaacc ggatcgtcta ccgcttccgg ccgtacaaga
     2581 accaccacga caagtcgatc atggagtgct actacctgag cccctgcgcc ggcgagcggc
     2641 cggagccggc ccccgtgcac tggctgggtg aggacgagga ctggaccgag gccactgagc
     2701 tcggaatcct cgcgaaagtc ttccagcagg acaccttcaa cctgcccaag gtgcagatcg
     2761 gcctggaagc cgcccagtac gaggagatcg tcctggccaa ctatcaggaa agcaagctgc
     2821 gtcacttcca cacgctgctg gaggagtgga tcgggcggta gatccgtaac caggcgcggt
     2881 gtttgtcatg aacttccaga t
//
LOCUS       DBNHOX010000114         5232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1295202, whole genome shotgun sequence.
ACCESSION   DBNHOX010000114 DBNHOX010000000
VERSION     DBNHOX010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1295202"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="AC16UZ_02505"
     CDS             complement(<1..317)
                     /locus_tag="AC16UZ_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6245580.1"
                     /translation="MDTDLATQVEQLADRVRGLSSASVDKPVLVYFDIIGICWPIRCL
                     LHLKGVDYELVQVPIQAWAFRDANGDQVLKACFRNGHMPLYVDPDVYLTQSSVIMYYL
                     GEK"
     gene            397..1146
                     /locus_tag="AC16UZ_02510"
     CDS             397..1146
                     /locus_tag="AC16UZ_02510"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6245581.1"
                     /translation="MTEELQQAHEALIRGEGFTVLSSLMTEGEAARVRDSILNHLEQA
                     VDERDGVARLSNLLAVDTSFDAYVTNPMLLGLAHRMLGPDARLAALGARVLMPGCELG
                     GLHVDYPYWAMDPGMPVEPALMLQVIWMLEPFTKDNGGTWVAPGSQAWGEYPSEKRFA
                     DSAVQITGNAGDAVVSHGLLWHRTAKNRTDEPRVALLVNYSQLAIQPMTPMGPFTDEY
                     RAGASAELRALLGFDHGRALRNRAMNVAREG"
     gene            complement(1143..2258)
                     /locus_tag="AC16UZ_02515"
     CDS             complement(1143..2258)
                     /locus_tag="AC16UZ_02515"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR6245582.1"
                     /translation="MEGLETPWSMAWLPDGRMLITERRGELLRFDPEDESLTEIEGVP
                     AVRAEGQGGLFDVVLHPRYAETGWVYLAYAYLDAESGRSSTAILRARLEGDALIDQEP
                     LYRAEQDFGLVSDLKSSVKQMLGKPPGRDSRAHYGGRMVFDEAGYLYFSVGDRGSRDL
                     FPQDLTRDAGKIYRIHDDGRIPEDNPFLHREDAKPAIYAYGLRNPQGMARHPGTGKIW
                     IHEHGPRGGDELNVIEPGANYGWPILSHGVNYSGSEFAEGTEREGFRSPAWHWTPSIA
                     PSGMAFVTSPRYPEWRGGLLAGSLKFGYLVLCRLDGDRVTGQEIVVEDLGRLRDVREG
                     PDGFIYVAIDGRGIVRILSSVGAGESAADGAETIPGL"
     gene            complement(2375..3853)
                     /locus_tag="AC16UZ_02520"
     CDS             complement(2375..3853)
                     /locus_tag="AC16UZ_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245583.1"
                     /translation="MLERIRNLSLLRVATIYVVGGWIVIQFAQIAFEAFEVPNFVLQW
                     VMAIVFSGFPLVILALSMMGVPFRRSAAEEVEERDESREESAQPQQPVDPGDLPARDN
                     RPSIALLPFRYLGEGEAFLAEGLRMGLQTNMVRLSGLFQVYSLLNEAYVSGEVDGEVR
                     GRDANAQYVLEATVHELTGRVRISLQLTEVATGRCAWAEQYDRDLEDLFELQDEIMKE
                     VIASLHIRLRNAGIEDSWFDQLRSAVAKEWYFRGANHLAGMSRETNAAAREAFEKLHE
                     LEPESAIGSSNVALTYWNDFAAGWGPDRDEALAHAIEWAKLARSYDRNDGIGDAVYAY
                     EQLGRRNYEGAMEAARRSVRVRPNCPISLGVLAQVENCSGNHDAAAGLAEIAQSKVEV
                     PFPWVCNVLATAQRDGGQLERSLLTLERALQAAPDDVDLLVTKCSDHALLSDLESAES
                     AASRILSVEPEWSTAQYAAEALYRSPDELDRVVAALRSAGLP"
     gene            4095..4532
                     /locus_tag="AC16UZ_02525"
     CDS             4095..4532
                     /locus_tag="AC16UZ_02525"
                     /inference="COORDINATES: protein motif:HMM:NF018717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR6245584.1"
                     /translation="MEVINQLYPTRKDQIEKMMEPGPDGPIVMVNLLKFREKAEYEDG
                     RETDLTGREAYMLYGAAVSKLITEYGGRLVFAGDVTNLTIGEVEELWDEIALAEYPNR
                     AALVAMSTSEAYAKIAAHRTAGLAGQLNIETTYQAGLTQPLRG"
     gene            4683..>5232
                     /locus_tag="AC16UZ_02530"
     CDS             4683..>5232
                     /locus_tag="AC16UZ_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245585.1"
                     /translation="MRKILTSIAAATLLFGISLSAAAAPGWLATGLTVMPENAPKVVA
                     ALDALFDSKVGRKLPGRVILRANLADGNNPETHAIVSLFRSAAESEAYRAELWADPAW
                     GEFMAAMRGLNETAGSTMRGMIAYNSGERADSDVVWVNHYVTVSEPAVLLSAMRSYSQ
                     SATGQAAPGQVHLSAVTAGGVGS"
BASE COUNT         1003 a         1744 c         1616 g          869 t
ORIGIN      
        1 tgcttctcgc ccaggtagta catgatcacg ctgctctggg tgaggtagac gtccggatcg
       61 acgtaaaggg gcatgtgccc gttgcggaag caggccttga gcacctgatc accattcgcg
      121 tcgcgaaagg cccaggcctg tatcggcacc tgcaccagct cgtaatcgac gcccttcagg
      181 tgcagcaggc agcgtatggg ccagcagatg ccgatgatgt cgaagtagac gagcacgggc
      241 ttgtcgacgc ttgcgctgga cagcccgcga acccggtctg caagctgctc gacctgcgtg
      301 gcgagatcag tatccatggc ccgttcccct tcccttgctt tggagcgaag cataccccat
      361 gccgtgctag tctctcccct gtcagggggc tcgaaaatga ccgaagagct gcaacaggca
      421 cacgaagcgc tgatccgggg cgaaggcttc acggttctct ccagcctgat gacggagggc
      481 gaagcggccc gcgttcggga ctcgatcctc aatcatctgg aacaggccgt ggacgagcgc
      541 gacggggtcg cgaggctttc gaacctgctg gcggtggata cgtccttcga tgcgtacgtt
      601 accaatccca tgctgctggg actcgcccac cggatgctcg gccccgacgc gcggctggcc
      661 gcccttgggg cccgggttct gatgccgggc tgcgagctcg gcggcctgca cgtggactat
      721 ccttactggg ccatggatcc cggcatgccg gtggagccgg ccctgatgct ccaggtgatc
      781 tggatgctcg agcccttcac caaggacaac ggcggcacct gggtggcgcc cggcagccag
      841 gcctggggcg agtacccgtc ggagaaacgt tttgcggatt ccgccgtgca gatcaccggc
      901 aacgcgggtg acgccgtggt gagccacggt ctgctgtggc accgaacggc caagaaccgg
      961 acggacgagc ccagggtggc cctgctcgtc aactactccc agctcgccat ccagcccatg
     1021 acgcccatgg gtcccttcac ggacgagtac cgggcaggcg catcggccga gctgcgggcg
     1081 ctgctcgggt tcgaccacgg cagggccctg aggaatcggg ccatgaacgt cgcccgggag
     1141 ggttagagcc ccgggatcgt ttccgctccg tcggccgccg actcgccggc gccaacggac
     1201 gaaaggatcc ggacgatgcc ccgaccgtca atggccacgt agatgaaacc atccggccct
     1261 tcccggacgt cgcggagtcg acccagatct tcgacaacga tctcctgccc ggtcacgcga
     1321 tcaccgtcca gccggcacag caccaggtaa ccgaacttca gcgagccggc cagcaggcca
     1381 ccccgccatt cgggatatcg cggcgaggtc acgaaagcca tgccggaggg cgcgatggac
     1441 ggcgtccagt gccaggccgg tgagcgaaac ccctcccgct cggtgccttc ggcaaactcg
     1501 ctgccgctgt agttcacacc gtggctgagg attggccacc cgtaattggc cccgggctcg
     1561 atcacgttga gctcgtcgcc gcccctggga ccgtgctcgt gaatccagat cttccccgtc
     1621 ccgggatggc gggccatgcc ctggggattg cgaagcccgt aggcgtagat ggccggcttc
     1681 gcatcctccc gatgcaggaa cgggttgtcc tcggggatcc ggccatcgtc gtggatccgg
     1741 tagatcttac ccgcgtcccg ggtcaggtcc tggggaaaca ggtcccggct gccccggtct
     1801 cccacggaga agtacagata tcccgcttca tcgaagacca tgcggccgcc gtagtgggct
     1861 ctcgaatcgc gcccaggcgg cttgccgagc atctgcttga cggaactctt gagatccgag
     1921 accaatccga agtcctgctc cgcgcggtaa aggggttcct ggtctatcag cgcatctccc
     1981 tcgagccgcg cccgaaggat cgccgtgctg cttctgcccg actccgcatc gaggtacgcg
     2041 tatgccaggt agacccagcc tgtctccgcg tagcgcggat ggagcaccac gtcgaagagc
     2101 ccgccctgac cctcggcccg caccgcggga acaccctcga tctcggtaag cgattcgtct
     2161 tcggggtcga agcgcagcag ctcaccccgg cgctccgtga tgagcatgcg gccgtccggc
     2221 agccaggcca tgctccaggg cgtttccaga ccttccacga cggtctccac ctcataggtt
     2281 cccgcgtggg aaaggccgac gcacaaccac agaagcaggc cccagacggc cctccggaaa
     2341 ggccgagctt cgaaccaacc tccaaaacgg cccctcatgg caaacccgca gatcgcagcg
     2401 ccgcaaccac ccgatcgagc tcatcgggcg atctgtagag cgcttctgct gcgtattggg
     2461 ccgtcgacca ctcgggttcc accgagagaa tcctgcttgc agcggattca gcggactcga
     2521 gatcagacag cagcgcatgg tcactgcact tcgtgacaag caggtcaacg tcatcgggcg
     2581 cggcctgcaa agcgcgctcg agggtcagca aagaacgttc gagctgacca ccgtccctct
     2641 gagccgtcgc caggacgtta catacccatg ggaaaggcac ctccaccttg gattgggcaa
     2701 tctccgccag accggcagcc gcatcgtgat tgccgctgca gttctcgacc tgagcgagca
     2761 cgcccaggga gatcgggcag ttgggccgga ctctgacgct gcggcgcgcc gcttccatgg
     2821 ccccctcgta atttcgccga ccaagctgct cgtaggcgta cacggcatcc ccgatcccgt
     2881 cattgcgatc gtaagacctc gcaagcttgg cccactcgat cgcgtgggcc agggcctcgt
     2941 cccgatccgg gccccaaccg gcagcaaagt cgttccagta ggtgagcgca acgttgctcg
     3001 agccgatggc ggactccggc tcgagctcgt gcagcttctc gaacgcttcg cgggccgccg
     3061 cgttggtctc gcgggacata ccagccagat ggttcgcacc acggaaatac cactccttgg
     3121 ctaccgcgct tcgaagctgg tcaaaccacg agtcttcgat gccggcattg cgcagacgga
     3181 tgtgcagtga ggcgatcacc tccttcatga tctcatcctg cagctcgaag agatcttcta
     3241 aatcgcgatc atactgctct gcccacgcac acctgcccgt cgcaacttcg gtgagctgca
     3301 gggatattcg cactcgcccg gtcagctcgt gaaccgtcgc ttccagaacg tattgcgcgt
     3361 tggcatcgcg ccctctgacc tcgccatcga cctcgccgct gacataggcc tcgttcagaa
     3421 ggctgtaaac ctgaaacaaa ccggagaggc gtaccatgtt ggtctgcagc cccatgcgca
     3481 ggccttcggc gaggaacgct tccccttcgc ccaagtacct gaatggcagc agggcaatcg
     3541 acggtcgatt gtcgcgcgcc ggaaggtctc caggatccac cggctgctga ggctgtgcgc
     3601 tctcttcccg ggactcgtca cgctcctcga cttcctcggc ggcggaccgg cgaaagggca
     3661 cgcccatcat gctcagggcg agaatcacca ggggaaatcc tgaaaacacg atcgccatga
     3721 cccattgcag gacgaaatta ggcacctcga aggcttcaaa cgcgatctgt gcaaactgga
     3781 tgacgatcca gccgcccacc acgtagatcg tcgcgacccg cagcagactg agattccgga
     3841 ttcgctctag cacttcgtca tgacgttcaa gaaattaggg ggcaagaccg ggagtggatt
     3901 tcagcagcgt tccgaatccg aaggctaccg cagaatacgc cacgacgcga cgccggcacg
     3961 gcgccccttc agccagcacg ttcagaccag ggaaaccggc ccggttgcgc gtgacatcgt
     4021 gtaatgatta ccattatttc aacgcgcccc gcggtcgcta tggagcccga gggtatcagc
     4081 cagaggaacc cgatatggaa gtgatcaatc agctctaccc cacccgaaag gaccagatcg
     4141 agaagatgat ggagccgggg cccgatggtc ccatcgtcat ggtcaacctg ctgaagttcc
     4201 gggaaaaggc ggagtacgaa gacggccgcg aaacggacct gaccggacgc gaggcctaca
     4261 tgctctacgg cgcggcggta tcgaagctca tcaccgagta cggtggccgg ctcgtcttcg
     4321 caggggacgt gaccaacctg accatcggcg aggtggagga gctgtgggac gagatcgccc
     4381 tggcggagta ccccaatcgc gccgccctgg tggccatgtc cacctccgag gcctacgcga
     4441 agatcgccgc ccaccgcacc gccggcctgg cgggccagct caacatcgag acgacttacc
     4501 aggcgggcct tacccagcca ctgcgggggt agccctggcg catgcatgca gggcaacgga
     4561 cgcttcgggg aacggatacc ccggcccagg ggcgaaaaga cgggccaaac gtcgctctcg
     4621 ttcgctatag tgaccggcac ctgcctcggg aaggggcaga tcaataaagg aaaatactaa
     4681 ccatgagaaa gatcctgact tcaatcgccg cagcaactct gctgttcgga atcagcctca
     4741 gcgcggccgc cgcaccggga tggctcgcca ccgggctgac ggtcatgccc gagaacgccc
     4801 ccaaggtggt ggcggccctg gatgcgctgt tcgactccaa ggtcggacgc aagctgcccg
     4861 gccgcgtgat cctgcgggca aacctggcag acgggaacaa tccggaaacc cacgccatcg
     4921 tatcgctctt ccgatcagcg gctgagagcg aggcctatcg cgccgagctc tgggccgacc
     4981 ccgcctgggg ggagttcatg gccgcgatgc gcgggctgaa cgagaccgca ggcagcacaa
     5041 tgcggggcat gatcgcctac aacagcggcg agcgagcaga ctccgacgtc gtctgggtga
     5101 atcactacgt gacggtttcc gagccggccg ttctgctgag cgccatgcgc agctacagcc
     5161 agtcggcaac cggccaggcc gctccgggtc aggtgcatct cagcgcggtc actgccggcg
     5221 gggtggggtc gg
//
LOCUS       DBNHOX010000115         4059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_9082, whole genome shotgun sequence.
ACCESSION   DBNHOX010000115 DBNHOX010000000
VERSION     DBNHOX010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_9082"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="AC16UZ_02535"
     CDS             complement(<1..594)
                     /locus_tag="AC16UZ_02535"
                     /inference="COORDINATES: protein motif:HMM:NF016158.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIR6245586.1"
                     /translation="MSKVFLSFGNQLFHPRYLREHADAEFVMVEDEQFCRRYAFHKQK
                     LTFVLSAMRSYAQTLRGQGWRVRYFDLDSAATWRSAVREAVAATSADTLCHFELESPD
                     LTVAIARFAEQLNLKLRVIRTPMFINTLDDFRAHLDKHKTPKLLPFYKEQRLQHRILV
                     NGDGLPVGGRWNFDQFNREKLPRDLNPQEPALLSRAPL"
     gene            complement(619..1353)
                     /locus_tag="AC16UZ_02540"
     CDS             complement(619..1353)
                     /locus_tag="AC16UZ_02540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6245587.1"
                     /translation="MGKDLSRKVAVVAGAAGGLGEALSRALVAHPDYASVFALSRTRP
                     SSDDGIDWFETDTTSPVDLESAASRVAQLTDRVHLLICCSGVLHGGAGSPHRPEKSLA
                     ELELENLQLAVRVNAFAPLAAIRAFRDLLRHDQRPVAAVLSAMVGSIGDNSLGGWYSY
                     RMSKAALNMGVRNVAIEFGRLRNGPSVVAVHPGTTLTPLSEPFVRGRDPRTPDESARH
                     ILSVVHDLSPEDSGRFFNWDGRELPW"
     gene            complement(1521..2336)
                     /locus_tag="AC16UZ_02545"
     CDS             complement(1521..2336)
                     /locus_tag="AC16UZ_02545"
                     /inference="COORDINATES: protein motif:HMM:NF021623.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR6245588.1"
                     /translation="MHPITVRKMEFDVPGPEDFEPKWAAGDIVLSYVSTGVSLYVAYL
                     EPFLVKSLRRVLDQISDPELKENVDRFCRQEAQHYMQHERFNEAILQFGYPGLAERFA
                     QLKADFERFQATKDDKWCVGFVEGFEAYTTQTALKAFAARVPDHPKTDRRFGELFKWH
                     LLEEIEHRNVAYDIYEHLYGDQLFRAGMCWIAQTHIWRFILDCMKIMSPADVARFGET
                     YRVSGLTRMAAVAAVAPMFLKTYMPWYSPHKYVIPPEVAELSATLTAAAKSAA"
     gene            complement(2401..3180)
                     /locus_tag="AC16UZ_02550"
     CDS             complement(2401..3180)
                     /locus_tag="AC16UZ_02550"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6245589.1"
                     /translation="MRLSADEAERYARDGFLIRECAFTEQELSSLRLAAERAADAAWR
                     LSRSGRTYVLDGNRFVDAGHVTVQFEHAAGSETIRVVEPVAELDPDLNALVDDARICE
                     PMRDLVGSEEIALWTDKLNLKRPREGSGFGWHQDSPYWIHECRHVDHLPNVMVTLDDA
                     SEANGCFRVVRGSHKRGCLPGRADGTQLGGFYTDPDAFDARDQVPMVVAAGSLVFFSP
                     HSVHGSLPNRSDRPRRALVMTYQPAGHPMLKSGLVRNVMGG"
     gene            complement(3204..>4059)
                     /locus_tag="AC16UZ_02555"
     CDS             complement(3204..>4059)
                     /locus_tag="AC16UZ_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR6245590.1"
                     /translation="RGNTILLDRARPGAFELVKGDPDTVLFEDLPAGEKQCELWLPHN
                     AYVELRELTLDDGAFLAEPALDPRPRWVHYGSSISHCMEADHPGLIWPAVAARRAGAS
                     LTNLGFGGQCHLDQFVARTIREVDADVISIKTGINVINRDSMRERVFTPALHGFLDTV
                     REGKPDTPIILISPIYCPSAESRPGPTFPGRDGRFFTVSGHDEIREGCLTLRRVREII
                     EEVVEQRVAGGDRALAYVDGLDLFGAADAEDLPDDLHPNAAGYVRMGERFAPALADVL
                     SSPETGGR"
BASE COUNT          746 a         1302 c         1297 g          714 t
ORIGIN      
        1 cagcggcgcg cgcgaaagca acgccggttc ctgggggttg agatcgcgcg gcagcttttc
       61 acggttgaac tgatcgaagt tccagcgccc gccgaccggc agcccgtcgc cgttcaccag
      121 gattctgtgc tggagcctct gctctttgta gaacggcagc agcttcggcg ttttgtgctt
      181 gtcgagatgg gcgcggaagt cgtccagggt gttgatgaac atgggcgtcc gaatgacccg
      241 cagcttcaag ttcagctgct cggcaaaacg tgcgatggca acggtcagat ccgggctttc
      301 gagctcgaag tggcatagcg tgtccgcgct ggtcgccgcg acggcctctc ggaccgccga
      361 ccgccaggtc gctgcgctgt ccagatcgaa atatcggaca cgccagccct gcccgcgcag
      421 cgtctgcgcg tagctgcgca tggcggaaag aacgaaggtg agcttctgct tgtggaaagc
      481 gtatcgccga cagaactgct cgtcctccac catgacgaac tccgcatctg catgctcacg
      541 cagatatcgg gggtgaaaga gctgattgcc gaagctgaga aagaccttac tcaccgactt
      601 gcctccgttg ctgatggatc accagggcag ctcacgcccg tcccagttga agaacctgcc
      661 cgagtcctcg ggcgatagat cgtgcacgac ggacagaatg tgccgggcgc tttcgtcggg
      721 cgtacgcgga tcgcgcccgc gaacgaacgg ctcggacagc ggggtcaggg tggttccggg
      781 gtgcacggcc accacgctgg gaccgttgcg cagccgaccg aactcgatcg ccacgttgcg
      841 aacccccatg ttgagggctg ctttgctcat gcggtagctg taccagccgc cgagagaatt
      901 atcaccaatg ctccccacca tggcggagag cacggcagcg acggggcgct gatcgtggcg
      961 cagcaggtct cgaaaagccc ggatggcggc cagcggggcg aacgcgttga cgcggacggc
     1021 gagctggaga ttttcaagct ccagctcagc gagcgacttt tcgggtcgat gagggctccc
     1081 ggccccgcca tgcagaacgc cgctgcagca aatgagcagg tgcacccgat cagtgagctg
     1141 cgccacccga gacgcggcgc tctccagatc caccgggctg gtcgtgtccg tttcaaacca
     1201 atcgatacca tcgtccgacg atggcctggt gcgggacagc gcgaagacgc ttgcgtagtc
     1261 gggatgggca acgagcgccc ggctgagcgc ctcgcccagc ccccctgcgg cgccggccac
     1321 gacggcgact tttctcgaca aatcctttcc cattggctct ttctacagga aaaaacctat
     1381 acaaacaaca gaaaaactgg tgtttggttc cctgtatctc gaatttgtct atgtattgtc
     1441 gaatttatcg aagcggccct tcgcccggcg gaaaccgcag gcgcccggcc ggccggcttc
     1501 tctcctccct ccggtctggt tcaggcagcc gacttcgcgg ccgccgtcag agtcgcggac
     1561 agctcggcaa cctccggcgg aatcacgtac ttgtgcgggc tgtaccaggg catgtaggtc
     1621 tttaagaaca tgggcgcgac cgcagcaacc gccgccatcc gcgtaaggcc tgaaacccgg
     1681 taggtctcac cgaagcgggc cacgtcagcc ggcgacatga tcttcatgca gtccaggatg
     1741 aaccgccaga tgtgggtctg cgcgatccag cacatcccgg cacggaaaag ctgatcgccg
     1801 tagaggtgct cgtagatgtc gtaggcgacg tttctgtgct ctatctcctc gagcaggtgc
     1861 cacttgaaga gctcgccaaa gcgacggtct gtttttggat gatccggaac gcgcgccgcg
     1921 aacgccttga gcgcggtctg ggtggtgtac gcctcgaagc cttccacgaa gcccacgcac
     1981 cacttgtcgt ccttggtggc ctggaaccgc tcgaaatcgg ccttcagctg cgcaaagcgc
     2041 tccgccagcc ccgggtagcc gaactgcaaa atggcctcgt tgaaccgttc gtgctgcatg
     2101 tagtgctggg cttcctggcg gcagaagcgg tccacgttct ccttgagctc agggtcgctg
     2161 atctggtcca gaacccggcg cagggacttg accagaaacg gttcgagata ggcgacgtac
     2221 aggctcacgc cagtgctgac gtaggaaagg acgatgtccc ctgccgccca cttgggctcg
     2281 aagtcctcgg gaccgggcac gtcgaactcc atctttcgta cggtgatggg atgcatgagg
     2341 ctgtatcacc tgctccggag agaacgggaa ttatacacgc gggaacgaac ggtcatgggc
     2401 tcatcctccc atgacgttgc gcaccagacc ggatttcagc atcggatggc cggcgggctg
     2461 ataggtcatc acgagggctc gacgtgggcg atcggagcgg ttgggcagcg agccgtgcac
     2521 cgagtggggg ctgaaaaaga ccagcgagcc agcggccacc accatgggca cctggtccct
     2581 ggcgtcaaag gcgtccggat ccgtgtagaa cccgccgagc tgggtgccgt ccgcgcgacc
     2641 cggcagacat cctcgtttgt gacttccccg caccacccgg aaacaaccgt tggcctcgct
     2701 ggcgtcgtcg agggtcacca tcacgttggg cagatggtcg acgtgtcggc actcgtgtat
     2761 ccagtagggc gaatcctgat gccagccgaa gccggagcct tcccgcgggc gcttgaggtt
     2821 gagcttgtcc gtccacaggg cgatctcctc cgagccaacc aggtcccgca tcggctcgca
     2881 gatacgcgcg tcgtcgacca gggcgttcag gtccgggtcg agctccgcaa cgggctccac
     2941 gacgcgaatc gtctcgctgc cggcggcgtg ctcgaactga acggtgacgt gtccggcatc
     3001 gacgaaacgg ttgccgtcca ggacgtaggt ccttccgctg cggctcaggc gccaggcggc
     3061 gtccgccgcc cgctccgccg ctagccgcag tgacgagagc tcctgctccg tgaatgcgca
     3121 ctcgcggatc aggaaaccgt cacgcgcgta gcgctcagcc tcatcggcac tgagccgcaa
     3181 gcccctctga ctggacgccg gcgtcaacgg ccgccggttt ccgggctgct cagaacgtcg
     3241 gcgagcgcag gcgcaaaacg ctcgcccatg cgaacgtatc cagcagcgtt cgggtgcaga
     3301 tcgtcgggca gatcctcagc atcggccgca ccgaagaggt cgaggccgtc aacgtaggcg
     3361 agcgcccggt caccgccggc aacacgctgc tcgaccacct cctcgatgat ctcccgcacc
     3421 cggcgcagcg tcaggcaacc ctcacgaatc tcgtcgtgac cgctgaccgt gaagaaccgg
     3481 ccatcccggc cggggaacgt gggtccgggc cgggattcgg cgctggggca gtagatgggg
     3541 gagatcagga tgatgggcgt gtccggtttt ccttccctga cggtgtccag aaacccgtgc
     3601 agcgccggcg tgaacacccg ctcgcgcatg gagtcccgat tgatcacgtt gattccggtc
     3661 ttgatgctga tcacgtcagc gtccacttca cgaatggtcc gggcgacgaa ctgatccaga
     3721 tggcactgcc cgccgaaacc caggttcgtc aggctggcgc ccgcccgccg cgcggccacg
     3781 gcgggccaga tcagtccagg atgatctgcc tccatgcaat gactgatgga gctgccgtag
     3841 tgaacccagc ggggacgcgg atcgagggcg ggctctgcga ggaacgcccc gtcgtcgagc
     3901 gtcagctcac gcagctccac ataggcgttg tgaggcagcc acagctcgca ttgcttttca
     3961 cccgcgggca gatcttcgaa gaggaccgtg tcggggtcgc ccttcacgag ctcgaacgcg
     4021 ccgggccggg cgcggtcgag cagaatcgtg ttgcccctg
//
LOCUS       DBNHOX010000116         1738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_716925, whole genome shotgun sequence.
ACCESSION   DBNHOX010000116 DBNHOX010000000
VERSION     DBNHOX010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_716925"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1738)
                     /locus_tag="AC16UZ_02560"
     CDS             complement(<1..>1738)
                     /locus_tag="AC16UZ_02560"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR6245591.1"
                     /translation="NGNAAADLGERFVAQDALHRAFGVPASYDLPESPGDWVVDAYLE
                     GLNAYVDTLDDPPPEFRHTGALPRRFTRADVAARYRFTNFFQHRSWPNKLMLGRLMAT
                     HGVEWFRRHVLRFSDEDESLIETLREPLQTLNLKPINLLFPDAPVSGSNNWVVTGRLS
                     ASGKPMLGMDPHQAYSIPNTFFYVHLHAPGWDVFGASFPGVPYFMMGYTKKIAWGLTT
                     GFVDNYDVYVEEIAGDRYRTPDGWAEVERRTETIGVRGGTTREVEIASTRHGPLLEPL
                     TDALEMTANGPRRYQTAVSWNLTHLETSAGALALLPLAEDAAGFGGALFEGDVCPLVN
                     NIICVDTKDDIRRFVAATLPARKGVTGMVPLPGWDPDCDFRWSRADELLVEQAPASGF
                     ALTANNDTMGDAGPFPIHNFPTLPARAERIRDLLEAGGSADGKFTPADFERMQLDLYD
                     YRARDQIPGILMALEGREDDDLELAAAILRDWDCQATGESAAACLYYPFVDRVWQRTF
                     MMAVLDDDVLRGIPLAAPGLNRFDVDAFLAEGSPWRAHRELLFLTMAEVMKGVVQDVR
                     KDLGEPDTWAWGR"
BASE COUNT          289 a          563 c          600 g          286 t
ORIGIN      
        1 cggccccagg cccaggtgtc gggctcgcca aggtcttttc tcacgtcctg cacgacgcct
       61 ttcatgacct cggccatggt caggaacagc agttcccggt gcgcgcgcca tggtgatccc
      121 tcggcgagaa acgcgtccac gtcgaagcgg ttgaggcccg gcgccgccag cggaatgccc
      181 cgaaggacgt cgtcgtccag cacggccatc atgaacgtcc gctgccagac tcggtccacg
      241 aacgggtagt agaggcatgc ggccgccgac tctccggttg cctggcaatc ccagtcgcgc
      301 aggatggccg cggcgagctc gaggtcgtcg tcctcgcgac cttccagcgc catgaggatc
      361 ccgggaatct gatcccttgc ccggtagtcg tagaggtcca gctgcatgcg ctcgaaatcg
      421 gccggggtga acttcccgtc cgcggagccg cccgcttcga gcaggtcgcg aatccgctcc
      481 gcgcgcgccg gcagcgtcgg aaagttgtgg atgggaaagg gccccgcatc gcccatggtg
      541 tcgttgttcg ccgtcagcgc gaagcccgag gcaggcgcct gctcgaccag aagctcatcg
      601 gctcgcgacc accgaaagtc gcagtcggga tcccatccgg gaagcgggac catccccgtc
      661 acgcccttgc gtgcgggcag ggtcgccgcg acgaagcgcc tgatgtcgtc cttggtgtcg
      721 acgcagatga tgttgttgac aagcgggcag acgtcgcctt cgaacagcgc gccgccgaat
      781 ccggcggcat cctcggcgag cggcagcagg gcaagcgcgc cggccgacgt ctcaagatga
      841 gtgaggttcc agctcaccgc ggtctggtag cgccgcggcc cgttggcggt catttcgagc
      901 gcgtcggtca ggggctcgag cagcggtccg tgacgcgtcg aggcaatctc gacctcccgc
      961 gtggtgccgc cccggacccc gatggtctcg gtccgccttt ccacctcggc ccagccatcg
     1021 ggcgtgcggt agcggtcccc ggcgatctcc tcgacgtaga cgtcgtagtt gtccacaaac
     1081 ccggtggtga gtccccaggc gatcttcttc gtgtagccca tcatgaagta gggcacgccg
     1141 ggaaacgaag cgccgaacac gtcccagccg ggcgcgtgga ggtgcacgta gaagaacgtg
     1201 ttcgggatcg agtaggcctg atgtggatcc atccccagca tgggctttcc ggaagcggac
     1261 agcctgccgg tcaccaccca gttgttgctg ccggataccg gcgcatccgg gaagagcagg
     1321 ttgatgggct tgagattgag cgtttgcagg ggctcgcgca gggtctcgat gaggctttca
     1381 tcctcgtcgg agaagcgaag cacgtggcgg cggaaccact ccacgccgtg ggtggccatc
     1441 agccggccga gcatgagctt gttcggccac gaccggtgct ggaagaagtt ggtgaaccgg
     1501 tagcgcgcgg caacgtcggc ccgcgtgaag cgccggggca gggcgcccgt atggcggaac
     1561 tcggggggag ggtcatcgag ggtgtcgacg taggcgttca atccctcgag ataggcgtcc
     1621 accacccagt caccgggact ctcgggaaga tcgtagctgg caggcacgcc gaaggcgcga
     1681 tgcagggcgt cctgagccac gaaccgctct cccaggtcgg cggcggcatt gccgttgg
//
LOCUS       DBNHOX010000117         3506 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_138165, whole genome shotgun sequence.
ACCESSION   DBNHOX010000117 DBNHOX010000000
VERSION     DBNHOX010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_138165"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /gene="ftsA"
                     /locus_tag="AC16UZ_02565"
     CDS             complement(<1..619)
                     /gene="ftsA"
                     /locus_tag="AC16UZ_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR6245592.1"
                     /translation="MASDSELTRIVGLDIGTSKVAAIVAELNLEGELEIIGLGTHPSR
                     GLKKGVVVNIESTVQSIQRAVEEAELMAGCHIENVYAGIAGSHIRSINSHGIVAVRDR
                     EVYPQDVDRVIDAAQAMAIPADQKILHILPQEYVIDNQEGVKEPLGMSGVRLEAKVHL
                     VTCAVNAAQNIEKCIVRCGLTVNDIILEQLASSYSVLTDDERDLGV"
     gene            complement(609..1313)
                     /locus_tag="AC16UZ_02570"
     CDS             complement(609..1313)
                     /locus_tag="AC16UZ_02570"
                     /inference="COORDINATES: protein motif:HMM:NF015739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR6245593.1"
                     /translation="MRRVSAVLLIVLAGAVVAALVWQMDRPIENVRVIGELTPGEQQQ
                     IEAAVLTGLDAGLLSVDLAEVRDRLRSLSWTRRVTVRRVWPPGLDIAVDKDVPVAVWG
                     DGAHLTSNGRVIAIPDAPAALPVFDCALASPRSALEVYLLLQESLPGAGRRISALRQT
                     SLGEWEVELRGGLRVALGDRDIAQRIARFGIVYERALKDRIETVDYVDARYTGGLAVG
                     WKDPLLAYEEPTANGI"
     gene            complement(1306..2694)
                     /gene="murC"
                     /locus_tag="AC16UZ_02575"
     CDS             complement(1306..2694)
                     /gene="murC"
                     /locus_tag="AC16UZ_02575"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR6245594.1"
                     /translation="MRRVRRIHFVGIGGAGMCGIAEVLLNQGYQVSGSDLRASETTAR
                     LERLGATVHVGHAAGHVEGKDVVVVSSAVAGDNPELRAAHARRIPVVARAEMLGELMR
                     YRHGIAVAGTHGKTTTTSLITAIFQTAGLDPTFVIGGLLNSTGTNAQLGAGRYLIAEA
                     DESDASFLHLKPMQAVITNIDRDHMGTYHNDFAELRGAFVRFVHRLPFYGSVVLCTDD
                     ANVRDVLPELARPMLTYGFAEDGDYRARDLDVNGASWRFVAERPDGHGDLEISLAVPG
                     EHNVLNALAAAAVATDEGIPDEAIVAGLSQFRGVGRRFEVFAQTPVGCARVTMVDDYG
                     HHPSEVAAVIQTARRVWPDRRLVMVYQPHRFTRTRDLYDDFVRVLSEVDALVLLEVYA
                     AGEEPIPGADGKALCQGLRQRGLVNPTYAADPEEALGLLPDIVRDGDVVLVQGAGSVN
                     QISLSLKEVADA"
     gene            complement(2714..>3506)
                     /locus_tag="AC16UZ_02580"
     CDS             complement(2714..>3506)
                     /locus_tag="AC16UZ_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HIR6245595.1"
                     /translation="AFTLQASEFAKLLVLVYVAGYLARFDEAVRESSIALLRPLMMVA
                     IVALLLLMEPDFGSVVVLGAAVCGMLFVAGTRLRHFLLLVVVAALLLAALALLQPYRM
                     QRLVSFTDPWSFAFGSGYQLTQALIAFGRGEVTGLGLGEGIQKLFYLPEAHNDFIFAV
                     IAEELGLLGTVAVVVLFGTLIARVLLIAGRAVAEERYFAGYLTYGVGLLLGVQCLVNM
                     GVNTGMLPTKGLTLPFVSFGGNSLLVCSTMLGLAFRAQMEAKGRG"
BASE COUNT          626 a         1193 c         1133 g          554 t
ORIGIN      
        1 agacgcccag gtcgcgctcg tcgtccgtaa gcacggagta gctcgaggca agctgctcga
       61 gaatgatgtc gttgacggtc aggccacacc gcacgatgca cttctcgatg ttctgcgcgg
      121 cgttcacggc gcaggtgacc agatgcactt tggcttcgag ccgcaccccc gacatcccga
      181 ggggctcttt caccccctcc tggttgtcga tgacgtattc ctggggaagg atgtgcagaa
      241 tcttctggtc cgccgggatg gccatggcct gggccgcatc gatcacccgg tccacgtcct
      301 ggggatagac ctcgcggtcc cgcacggcga cgatcccgtg ggagttgatg cttcgtatgt
      361 gactgccggc gatgcccgcg tagacgttct cgatgtggca gccggccatg agctcggcct
      421 cctccacggc gcgctggatg gactgcacgg tggattcgat gttcaccacc acgcctttct
      481 tcaatccccg tgatgggtgc gttccgagcc cgatgatctc gagctcccct tcgaggttga
      541 gctcggccac gatggcggcc accttgctcg tgccgatgtc gagaccgacg attcgggtca
      601 gctcactgtc agatgccatt tgctgtgggt tcctcgtatg caaggagcgg atctttccaa
      661 ccaaccgcga gaccgccggt ataccgcgca tcgacgtagt cgacggtctc gatgcggtcc
      721 ttcaacgccc gttcgtagac gatgccgaat cgggcgattc gctgggcgat atcccggtcg
      781 cccagcgcaa cccgaagacc gccgcgaagc tcgacttccc actcgccgag ggacgtctgc
      841 ctgagcgccg agatgcgtct tcccgcgccc ggcagggact cctgcagcag cagatagact
      901 tccagcgcgc tgcgcgggct ggccagcgcg cagtcgaaga cgggcagcgc ggcgggcgcg
      961 tccgggatcg cgatcacccg gccgttgctg gtgaggtgag caccgtcgcc ccagaccgcg
     1021 acaggaacat ccttgtcgac ggcaatgtcg agccccggag gccatacgcg gcggaccgtc
     1081 acgcgacgcg tccaggaaag gctccgcagc cggtcacgca cctcggcgag atccacgctc
     1141 agcaggcccg catcgaggcc ggtgagcacc gccgcctcga tctgctgctg ctccccgggg
     1201 gtgagctcgc cgatgacgcg cacgttctcg atgggccgat ccatttgcca caccagcgca
     1261 gccaccacgg ccccggccag cacaatcagg agcacggcgg acaccctacg catccgccac
     1321 ctccttgagg ctcagcgaga tctggttgac gctgccggcc ccctgaacga gcaccacgtc
     1381 gccatcccgg acgatgtcgg gcaggagccc cagggcttcc tcggggtcgg cggcgtaggt
     1441 ggggttgacg agcccgcgct gccgcaggcc ctggcacaac gccttgccgt ccgcgccagg
     1501 tatgggttcc tcgcccgccg cgtaaacctc cagcagcacg agggcgtcca cctcggagag
     1561 aactctcacg aaatcgtcgt aaagatccct ggtacgggtg aaccggtgcg gctgatagac
     1621 catgacgagc cgccgatcgg gccagacgcg gcgcgcggtc tgaatcactg cggccacttc
     1681 ggaaggatgg tggccgtagt cgtcgaccat cgtgacgcgg gcgcagccca ccggcgtctg
     1741 cgcgaaaacc tcgaaccgcc ggcccacgcc gcggaactgc gacaggccgg cgacgatggc
     1801 ctcgtccggt atgccttcgt cggtggcgac cgctgccgcc gcgagcgcgt tgagcacgtt
     1861 gtgctcgccg ggaacggcca gggagatctc caggtcaccg tgaccgtcgg gacgctcggc
     1921 gacgaaacgc cacgatgcgc cgttcacgtc gagatcccgg gcgcggtagt cgccgtcctc
     1981 cgcgaagccg taagtcagca tgggccgggc aagctcgggc agaacgtcgc gcacgttcgc
     2041 atcgtccgtg cacagcacga cgcttccgta gaagggcaga cggtgcacga accgcacgaa
     2101 cgcgccgcgc agctcggcaa agtcgttgtg gtaggtgccc atgtgatccc ggtcgatgtt
     2161 ggtgatcacg gcctgcatgg gtttgaggtg aagaaacgac gcgtcgcttt cgtcggcttc
     2221 cgcaatcagg taccggccgg cgcccagctg cgcattggtt cccgtgctgt tgagcaaacc
     2281 gccgatgacg aaggtggggt ccaggcccgc ggtctggaat atggccgtga tcaggctggt
     2341 agtggtcgtc ttgccgtggg ttccggcgac ggcaatgccg tgacgatagc gcatgagctc
     2401 cccgagcatc tccgcccgcg ccacgaccgg tatccgccgc gcgtgagcgg cgcgaagctc
     2461 agggttgtct cccgcgaccg cgctggatac cacgaccacg tccttgccct cgacgtgccc
     2521 cgcagcatgg cccacatgga cggttgcgcc gagccgttcc agtcgcgccg tggtctccga
     2581 cgcgcgaagg tctgatcccg acacctggta tccctggttg agaagcacct cggcaatgcc
     2641 gcacatgccg gcaccgccaa tgcccacgaa gtgaatgcgc cgcacccggc gcatctccgg
     2701 cacgtagtac gactcagcca cggcctttcg cctccatctg ggcccggaac gcgagcccca
     2761 gcatggtgct gcacaccagc aggctgttac caccgaagct cacgaatggc agcgtgaggc
     2821 ctttggtcgg caacatcccg gtgttgacgc ccatattgac aaggcactgc acgcccaaca
     2881 gcaggccgac gccgtaggtg aggtagccgg cgaagtatct ttcttccgcc accgcccgac
     2941 cggctatgag cagcaccctg gcgatcagcg tgccgaacag aacgaccacc gcgacggtgc
     3001 ccagcaggcc gagctcctcg gcaatcacgg cgaatatgaa gtcgttgtgc gcttccggca
     3061 ggtagaagag tttctggata ccctcgccaa gaccgagccc ggtgacctct ccacgcccga
     3121 aggcgatcag cgcctgggtg agctggtagc cgctgccgaa ggcgaacgac cacgggtccg
     3181 tgaagctcac cagcctctgc atgcggtagg gctgcagcag cgcaagggcg gccagcagca
     3241 gagccgcgac cacgacgagc aggagaaagt gacgaagccg ggtgcccgcg acgaagagca
     3301 tcccgcagac ggcggcgccg agaaccacga cggagccgaa gtcgggctcc atgagcagca
     3361 gcagcgcgac gatggccacc atcatgagcg gccgcagcag cgcgatggag ctctcccgca
     3421 ccgcttcgtc aaagcgggcc agatagccgg ccacgtagac cagcaccagg agcttggcga
     3481 actctgacgc ctgcagcgtg aaggcg
//
LOCUS       DBNHOX010000118         3429 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1230440, whole genome shotgun sequence.
ACCESSION   DBNHOX010000118 DBNHOX010000000
VERSION     DBNHOX010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1230440"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="AC16UZ_02585"
     CDS             complement(<1..354)
                     /locus_tag="AC16UZ_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR6245596.1"
                     /translation="MRHWPPSRTSPIGAADIIFKAMLVPAVLLAVAACTREAEPRWLL
                     RALPGEYPEIVYFVPTTSRAVAMTIDDGLDPETTPLILETLKAHGASATFFVLSDSIA
                     GREVLLQRILYEGHEI"
     gene            662..2230
                     /locus_tag="AC16UZ_02590"
     CDS             662..2230
                     /locus_tag="AC16UZ_02590"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007977712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR6245597.1"
                     /translation="MKSSRRTSTTSILLALLFASMSAWSQDKPNILVIWGDDVGWSNI
                     SAYNLGMMGYQTPNIDRIAEEGALFTHYYAHQSCTAGRSSFILGQHPFRTGLLTIGMP
                     GADHGIPDWSPTIADLLKNHGYATGQFGKNHLGDRDEHLPTNHGFDEFFGNLYHLNAE
                     EEPETYHYPKDPEFRARFGPRGVIKSTADGQIQDTGPMTRKRMETADEEFLGSAMDFI
                     DRSVKADKPFFVWMNTTRMHVWTRLKAEAEGVTEMGLYPDGMAEHDGHVGLLLGQLDR
                     LGIADNTIVIYSTDNGAEKVTWPDGGTTPFHGEKGTTWEGGMRVPAVVRWPGTIKPGT
                     KIHDIMSHEDWLPTLLAAAGENDVVQKLKKGHRANGKRWRVHPDGYNFLPYLKGEADA
                     PREEIFYFGQNGDLDAVRWRDWKVSFATFDGNIATGIREVANWPVITHLRSDPFEEMH
                     YESSMYLRWMADNIWLFVPIQAKVQEFLSTMGDYPFQEGQSLNAAGINYRSLKALQIL
                     QQLNQKGVLSPPTN"
     gene            2247..>3429
                     /locus_tag="AC16UZ_02595"
     CDS             2247..>3429
                     /locus_tag="AC16UZ_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR6245598.1"
                     /translation="MRAAAALAALLLITGIATVEAASRPKLILQITVDQLRADLPTRY
                     YERLGKGGFKYLWEKGIVFTNAHHAHANTETIVGHATLATGAHPSVHGMVGNLWFDRE
                     TGFTTYNVEDGDHRLLTEAADVDDATEIDPTQRAARSEGRSPAAILVTTFADELRSNT
                     NGRAKAIGVSVKDRGAISMAGHSGTAYWFSKATGGFVTSTYYLDRYPDWVSDFNAAGP
                     AAAYAGTAWELLREQDTYLFGEADDRPWETDVAGFGRVFPHAFGDPSSPYFATWLTLS
                     PAGDRLVLDFAKRAIVEESLGQDATTDYLSVSFSSTDYVGHVFGPSSLEAEDNILRLD
                     RTLAELFAFVAEQVGLADTLIVLSADHGAPDSPGYLNSLGIPGGYVDPDSWDKADAID
                     RI"
BASE COUNT          665 a         1099 c         1087 g          578 t
ORIGIN      
        1 gatctcgtgg ccctcgtaga ggattctctg cagcagaacc tcgcggccgg caatgctgtc
       61 gctcaggacg aagaacgtgg ctgaggctcc gtgagctttg agcgtctcga ggatcagagg
      121 cgtggtctcg ggatccagac cgtcgtcgat agtcatggcc accgcccggg aggtcgtcgg
      181 cacgaagtag acgatctcgg gatactcccc aggcagcgcg cggagcagcc accgtggctc
      241 cgcttcccgc gtgcaggcgg caacggccag cagcacggcc ggcaccagca tcgctttgaa
      301 gatgatgtct gctgcgccga tgggtgacgt gcggctcggc ggccagtgtc tcatcaggat
      361 tgcgtccgga cttcctggga gtggcgagtg tgattcgacg gccctctatc gtgtcagagt
      421 cgccgtcttc atgaaaccgg ccccccgtca tgaacttcgg ctccctgctg gttcggcctg
      481 accgtcccac cgacaaactt gcaatcccga gaccgggcgc taggctgtgc ggttttgcca
      541 agcgttggaa aagcgcagcg ggatcggccc tggtgcgcat tcaggaaccc gcggaagaag
      601 aacaaccaga cgctcaggcc ttcaccgact ctaactgcgc acggatttgg agatctcaca
      661 gatgaaatcg agtagacgga cgtcgaccac gtcgatcctg cttgcgctgc tgttcgcctc
      721 aatgtccgca tggtctcagg acaagccgaa catcctggtc atctggggtg acgacgtggg
      781 ctggagcaac atcagcgcct acaacctggg gatgatgggc taccagacgc cgaacatcga
      841 ccggatcgcc gaggagggtg cattgttcac ccattactac gcccaccaga gctgcacggc
      901 aggacggtct tcgttcatcc tgggccagca cccgttccgc acgggcctgc tgaccatcgg
      961 catgcccggc gccgatcacg ggatacccga ctggagcccg accatcgccg atctgctgaa
     1021 gaaccacggc tacgcgacgg gccagttcgg caagaaccat ctgggtgatc gggacgagca
     1081 cctgccgacc aatcacgggt ttgatgagtt tttcggcaac ctctatcacc tcaatgcgga
     1141 ggaagagccg gagacctatc actatccgaa ggacccggag ttccgggcgc gcttcgggcc
     1201 gcgaggagtg ataaagagca cggcggacgg ccagatccag gacaccgggc ccatgacgcg
     1261 aaagcgcatg gagacggcgg acgaggagtt tctcgggagc gccatggact tcatcgatcg
     1321 ttcggtgaag gccgacaagc ccttcttcgt ctggatgaac acgacgcgca tgcacgtgtg
     1381 gacgcgattg aaggcagagg ccgagggggt cacggaaatg gggctctacc cggacggcat
     1441 ggccgaacac gacggccacg tgggcctgct tctcggtcag ctggatcgcc tcggcatcgc
     1501 cgacaacacc atcgtcatct acagcaccga caacggcgcc gagaaggtca cctggccgga
     1561 cggcggcacg acgccgttcc acggcgagaa gggcactacc tgggaaggcg gcatgcgggt
     1621 gcccgcggtg gtgcgctggc cggggaccat caagcccggg acgaagatcc acgacatcat
     1681 gtcccacgag gactggctgc cgacgctgct ggccgcggcg ggcgaaaacg acgttgtgca
     1741 gaagctgaaa aagggccacc gggccaacgg caagcgctgg agggtgcacc cggatggtta
     1801 caacttcctg ccttacctca agggcgaagc ggacgcgccg cgcgaggaga tcttctactt
     1861 cggccagaac ggcgatctgg atgcggttcg ctggcgggac tggaaggtca gctttgcgac
     1921 cttcgacggc aacatcgcga cgggtatccg ggaagtggcc aactggccgg taatcaccca
     1981 tctgcgttcg gatccgtttg aagagatgca ctacgagtcg tccatgtatc tccgctggat
     2041 ggcggacaat atctggctgt tcgtgcccat ccaggccaag gttcaggagt tcctctcgac
     2101 catgggtgac tatccgttcc aggagggcca atccctgaac gccgcgggca tcaactaccg
     2161 atcgctgaaa gcgctgcaga tccttcagca gctcaatcag aagggcgtgc tgtcaccgcc
     2221 gacgaactga cgtggtagcg cgtcgattgc gcgcagcggc cgccctcgcg gcgctgctgc
     2281 tgattaccgg catcgcaacc gtcgaggcgg ccagccggcc aaagctgatt ctccagatca
     2341 cggtggatca gctcagggcc gatctgccga cccgctacta cgagcggctc ggcaaaggcg
     2401 gcttcaagta tctgtgggag aagggcatcg tcttcacgaa cgcccatcac gcacacgcca
     2461 acacggaaac catcgtcggc cacgccaccc tggctaccgg cgcgcacccc tccgttcacg
     2521 gcatggtggg caatctctgg ttcgaccggg aaaccgggtt cacgacctac aacgtcgagg
     2581 atggggacca ccggctgctc accgaggccg ccgacgtcga cgatgccacg gagatcgatc
     2641 ccacccagcg cgccgcccgc agcgaaggcc gctcgcccgc cgcgatcctg gtgaccacct
     2701 ttgccgatga gcttcgcagc aataccaacg gtcgcgcgaa agccattggc gtctcggtca
     2761 aggatagggg cgccatctcc atggcgggtc attccggcac cgcgtactgg ttttcaaagg
     2821 cgacgggcgg gttcgtcacc agcacctatt acctggaccg ttacccggac tgggtttcgg
     2881 acttcaacgc cgccgggccc gccgcggcat acgccgggac ggcttgggag ctcttgcgcg
     2941 aacaggacac ctacctgttt ggggaggccg acgatcggcc ctgggaaacc gacgtggcgg
     3001 gattcggccg ggtatttccc cacgcctttg gcgaccccag cagcccctac ttcgccacct
     3061 ggctgaccct gagcccggcc ggtgaccggc tggtgctcga cttcgccaaa cgagccatcg
     3121 tcgaggagtc gctggggcag gacgcgacca ccgactacct gagcgtgagc ttttcctcca
     3181 ccgactacgt tggccacgtc ttcggaccgt ccagcctgga ggcggaagac aacattctcc
     3241 ggctcgaccg aaccctggcg gagctgttcg ccttcgtcgc cgagcaggtg ggcctggccg
     3301 acacgctgat agtgctttcc gccgatcacg gcgcgccgga ttctcccgga tacctgaaca
     3361 gcctgggtat cccgggtggc tacgtcgacc cggacagctg ggacaaggct gacgccatcg
     3421 atcggatca
//
LOCUS       DBNHOX010000119         2585 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1488171, whole genome shotgun sequence.
ACCESSION   DBNHOX010000119 DBNHOX010000000
VERSION     DBNHOX010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1488171"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="AC16UZ_02600"
     CDS             complement(<1..551)
                     /locus_tag="AC16UZ_02600"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6245599.1"
                     /translation="MIARAPGRHALLFILITIFADTVAFGIIIPVLPKLIAELGDTSI
                     SEAASYGGLLMFVFAVMQFLFAPVIGNLSDRFGRRPVLLISLAALGIDYVIMGLAPSL
                     VWLFLGRMVAGVAAATFATANAYVADITPDEQRAARFGLIGAAWGVGFVLGPAIGGLL
                     GEMDMRLPVFAAAGLALANVVYG"
     gene            complement(606..836)
                     /locus_tag="AC16UZ_02605"
     CDS             complement(606..836)
                     /locus_tag="AC16UZ_02605"
                     /inference="COORDINATES: protein motif:HMM:NF044586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245600.1"
                     /translation="MSKGTTSQSSNADLRRQLDADVEKFLKSGKKIQQIPTGVSSQDY
                     TKGRKHIVLGKSKPRTPETRTPETRTPEKGSE"
     gene            complement(945..1352)
                     /locus_tag="AC16UZ_02610"
     CDS             complement(945..1352)
                     /locus_tag="AC16UZ_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6245601.1"
                     /translation="MQVEAGDVIPEWVMERVAPERMRTMAAILRDPNPVHWDRKAVAA
                     LGFGDHTINQGPLGLSYMINMLHEWAGPHCLKRVFMRFPQVVLDEDHVIARGVVTAVR
                     ELDGAREADCEIWLEREGAGRLLEGSAVVRLPD"
     gene            complement(1342..1791)
                     /locus_tag="AC16UZ_02615"
     CDS             complement(1342..1791)
                     /locus_tag="AC16UZ_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245602.1"
                     /translation="MTMPDASEIEALVGHEFPGGKYTVAHWENFLLTECTGAEQLPEG
                     MVHPVVMFHMPILGAGTSIGEMFALGQAESDYSIGIESYDWEFFGTLEEDVEYSISAR
                     VTEADRRRSGDRVYDRIQFQFDVADPGGTVLARSTITWTYGRSGDAS"
     gene            1913..2464
                     /locus_tag="AC16UZ_02620"
     CDS             1913..2464
                     /locus_tag="AC16UZ_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6245603.1"
                     /translation="MSKIDPYASWLPLEARAAAETNPRHKQLLLEVRDHMEHEIKGAL
                     EPLMATLTAEPVYHFWGNEPSVLNGRDAVRDFYKGMIAMGGQQFQVVVDRIVVDDGAV
                     VTEGQVKQVYKGAALKAMGMDAVGGERVADDDLFMTTAQLVTVWPADAVGKLIGEDIY
                     FGHDPFRNVERIGREDLPDYYQI"
BASE COUNT          507 a          856 c          808 g          414 t
ORIGIN      
        1 aacccgtaaa ccacgttggc cagggccagc cccgcggcgg caaagacagg cagcctcatg
       61 tccatctcgc cgagcagccc gccaatggcc gggccgagca cgaaccccac accccaggcc
      121 gctccgatca ggccgaagcg ggcagcccgc tgctcgtcgg gcgtgatgtc ggccacgtag
      181 gcgttggcgg tggcgaaagt cgccgccgcc acgcccgcga ccatgcggcc gagaaacagc
      241 cacacgaggc tgggcgccag ccccatgatc acgtagtcga tgccgagcgc ggccagggag
      301 atcagcagaa cgggccggcg accgaagcgg tccgaaaggt ttccgatcac cggcgcgaag
      361 agaaactgca tgaccgcgaa gacgaacatc agaagaccgc cgtagctggc cgcttcgctg
      421 atggaggtat ccccgagttc ggcgatcagc ttcggcaaca ccgggatgat gatgccgaac
      481 gcaaccgtgt ccgcgaagat ggtgatgagg atgaacagca gcgcgtgccg tcccggcgcg
      541 cgcgcgatca cgcgatcatg tcccgggctg gcacgccgac cccggcggga acatcagggt
      601 gacggctact cggacccttt ctcgggagtc cgcgtctcgg gagtccgcgt ctcgggagtc
      661 cgcggtttcg atttgccgag aacgatgtgc ttgcggccct tggtgtagtc ctgcgagctg
      721 accccggtcg gaatctgctg aatcttcttg cccgacttta gaaatttctc gacgtccgca
      781 tcgagttgcc ggcgaaggtc cgcattcgac gactgactgg tagtgccttt gctcatgatg
      841 aagagggtcc gctaacgttc ggcgacgacg gtattcgccg tcactggaag atgcatcatg
      901 cccgcacgga gcgagcaaag ccagcggacc tccccggaaa gcggctaatc cggcaaccgg
      961 acgacggccg agccttccag gagccggccc gctccctcgc gctccagcca gatttcgcaa
     1021 tccgcctcgc gagctccatc cagctccctc acggcagtga cgacgccgcg ggcgatcacg
     1081 tgatcctcgt cgagcaccac ctggggaaaa cgcatgaaca cccgcttgag acagtgcgga
     1141 ccggcccact cgtgcagcat gttgatcatg tagctcaggc ccagtggacc ctggttgatg
     1201 gtgtgatcac cgaagcccag cgcggcaacc gctttgcggt cccagtgcac ggggttggga
     1261 tcgcggagaa tggcggccat ggtgcgcatg cgctccggcg ccacgcgttc catgacccac
     1321 tccggaatga cgtcgcctgc ctcaacttgc atcgccgctc ctcccatacg tccaggtgat
     1381 ggtgctccgg gcgaggacgg tgccccccgg atcggccacg tcgaactgaa actgaatccg
     1441 gtcgtagacg cgatccccac tgcggcgacg atcagcctcg gtcacgcgtg cggagatcga
     1501 gtactccacg tcctcctcca gcgtgccgaa gaactcccag tcatagctct cgatgccgat
     1561 ggagtaatcg gattccgcct gacccagggc gaacatctcg ccgatgctcg tgccggctcc
     1621 gagaatcggc atgtggaaca tgaccaccgg gtgcaccatg ccctcgggca gctgctctgc
     1681 gcccgtgcac tcggtcagca ggaagttctc ccagtgagcg accgtgtatt tgccgcccgg
     1741 aaactcgtgg cccactagag cctcgatttc ggacgcgtcc ggcatcgtca tgccacattc
     1801 cccttcttga tccagcgaac aacattcaaa ccaatgaccg ggacctggtc aacggacggt
     1861 tcagcctgac gcgtcgggcc ggtaacatct gcagaccgca aaggagaccc agatgtcaaa
     1921 gatcgacccg tacgccagct ggcttcctct ggaagcgcgt gcagcggccg aaaccaatcc
     1981 ccggcacaag cagctgctgc tggaggtacg cgaccacatg gagcacgaga tcaaaggcgc
     2041 gctggagcca ctcatggcaa cgctgaccgc ggagcccgtc taccatttct ggggcaacga
     2101 gccgtcggtg ctcaacggcc gcgacgcggt cagggatttc tacaagggca tgatcgccat
     2161 ggggggtcag cagttccagg tagtcgtcga ccgcatcgtg gtggacgacg gcgccgtggt
     2221 taccgaaggg caggtgaagc aggtctacaa aggcgcagcc ctgaaggcca tgggaatgga
     2281 cgccgtgggc ggtgaacggg tggcggacga cgatctgttc atgaccaccg cccagctggt
     2341 caccgtctgg cccgccgacg ccgtcggcaa gctcatcggc gaggacatct acttcgggca
     2401 cgatccgttc cgcaacgtcg agcggatcgg ccgggaagat ctgcccgact actaccagat
     2461 ctgatgatcc ccaagaccaa gaccgcatac tacgggatcg gctggccgct aatgctgcta
     2521 gcccttacgg gccagacggc cctggcggcc agcgccgcgc accgcgccca gcacggcatg
     2581 gtggt
//
LOCUS       DBNHOX010000120         2246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_844982, whole genome shotgun sequence.
ACCESSION   DBNHOX010000120 DBNHOX010000000
VERSION     DBNHOX010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_844982"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="AC16UZ_02625"
     CDS             complement(<1..1064)
                     /locus_tag="AC16UZ_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6245604.1"
                     /translation="MKARAFVAAILVLTGASAGARENVAWPAYGGGPGGGHYSQADQI
                     TPDNVAGLEVAWQHRSGDYREGTIRGVEGFSGDAVAASAFIGTPIVVNDTLYYCTPFN
                     RVFALDPQTGAERWMFDPGVKHELEHLTNCRGVSSWTDPLASPDALCAHRIIAPTLDG
                     RIFAVDGKTGKRCEGFGRNGEIDLTEGLTEHGAQEYGITSAPAILNDILVTGSYVTDS
                     IRIDIPSGVVRAYDLRTGAFRWGWNPVPPGTAERDEAGNYRAGTTNVWSTISVDAERN
                     LVIVPTGNSSPDYYGGHRDGDLDYYSSSVVALDGDTGEVVWHYQTVHHDIWDYDLPAQ
                     PTLLDVTIDGKTRPAVVQVT"
     gene            1208..>2246
                     /locus_tag="AC16UZ_02630"
     CDS             1208..>2246
                     /locus_tag="AC16UZ_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6245605.1"
                     /translation="MASDQASGASARPDAAHSGPARPLSRRTLFYYGLADMPIQMAGI
                     PVAAFIPNYYGQDLGVSLGAVGTIWLLTRLFDAVSDPLIGWLSDRTDTRWGRRRVWMV
                     AAVPILMLSVYKLFMPDPAAVSAGYLLGWLVALWVGWTMLFIPYYAWAAELSPDYTER
                     TRITGWRSWIGMAANVVSKVVPVIAIYCCAFGGTEETLFLIGTMTLILLPVTVGLTVM
                     KVPEGRDYLPTRIPLARGLKLMWQNAPFKRLVLAFFINQLGSSISTALVVFYIRVVLQ
                     EDAMPIVFLLTYYLFNLSGIPLWVWFSGKVGKHRAWCVGLALFGTAQCGYLLLGPGDF
                     LYMLPIGALTGF"
BASE COUNT          334 a          745 c          731 g          436 t
ORIGIN      
        1 ttggtcacct gcaccacggc cggacgcgtc tttccgtcga tggtgacatc cagcagggtc
       61 ggctgcgcgg gcaggtcgta atcccagatg tcgtgatgca ccgtctggta gtgccagacg
      121 acctcgccgg tgtcgccgtc cagcgccacc acggagctcg agtagtagtc caggtcgccg
      181 tcccggtggc cgccgtagta gtcgggcgac gagttgcccg tcggcacgat caccaggttg
      241 cgttcggcgt ccacgctgat ggtggaccag acgttggtgg tgcccgcgcg atagttcccc
      301 gcctcgtcgc gctcggcggt gcccggcggt acggggttcc aaccccagcg gaacgctccg
      361 gtgcgcaggt cataggcacg cacgacgccc gagggtatgt cgatgcggat gctgtccgtg
      421 acgtaggagc ccgtgacgag aatgtcgttg agtatggctg gcgcgctggt aatgccgtat
      481 tcctgggcgc catgctcggt caacccctcg gtgagatcga tctcgccgtt gcggccgaag
      541 ccttcgcaac gcttacccgt ttttccgtcc acggcgaaga tacggccgtc cagggtcggg
      601 gcgatgatgc ggtgggcgca aagggcgtcg ggagacgcca gggggtccgt ccaggagctc
      661 accccgcggc agttggtgag gtgctccagc tcgtgcttca cgcccgggtc gaacatccaa
      721 cgctcggcgc ccgtctgagg atccagggca aaaacccgat tgaacggcgt gcagtaatac
      781 agcgtgtcgt tcacgacgat gggcgtgccg atgaaggccg aggcggccac cgcgtcgccg
      841 ctgaagccct ccacgccgcg gatggtgccc tctcgatagt cgcccgagcg atgctgccag
      901 gcgacctcca ggcccgccac gttgtccggc gtgatctggt cggcctggga gtagtggccg
      961 cctccgggtc cgccaccgta ggccggccag gcgacgttct cccgagcacc cgcggatgcg
     1021 cccgtaagca ccagaatggc agcgacgaaa gctctggctt tcatggtgaa tcccctcccg
     1081 atcatcgcgc ggctccagcc tgcgctgagc ctcattcaac cacccgctcc gccgcagatc
     1141 aaaaggaaaa cacgttatac tcggcgggac gtggtgcatc cgcttttcag ggtgagggga
     1201 gatccatttg gccagcgacc aggcgtccgg cgcatcggca cggcctgatg ccgcgcactc
     1261 cggcccggcg cggccgctgt cgcgcagaac cctgttctac tacgggctcg ccgacatgcc
     1321 catccagatg gcgggcattc ccgtggccgc cttcatcccc aactactacg gccaggacct
     1381 cggcgtcagc ctcggggcgg tcgggaccat ctggcttctc acgcgcctgt tcgatgcggt
     1441 atcggatccg ctgatcggct ggctgtccga ccgcacggat acgcggtggg ggcgccgacg
     1501 cgtttggatg gtggcagcag tgcccatact catgctgtcg gtctacaagc tcttcatgcc
     1561 ggatcctgct gcggtgagcg caggctatct gctgggctgg ctggtggcgc tctgggtggg
     1621 ctggaccatg ctgttcatcc cctactacgc ctgggcggcc gagctgtccc cggactacac
     1681 cgagcgcacc cgcatcacgg gctggcgttc ctggatcggc atggcggcca acgtggtcag
     1741 caaggtggtt cccgtgatcg ccatctactg ctgcgcgttc ggcggcaccg aggagacgct
     1801 gtttctcatc ggcaccatga ccctgatcct gctgcccgtc accgtgggcc tgaccgtgat
     1861 gaaggtcccc gaaggtcgcg actacctgcc cacccgtatt cccctggcgc gggggctcaa
     1921 gctcatgtgg cagaacgctc cgttcaagcg cctggtgctg gcgttcttca tcaaccagct
     1981 gggctcgtcc atctccacgg cgctggtggt gttctacatt cgcgtggtgt tgcaggagga
     2041 cgccatgcca atcgtgtttc tgctcaccta ctacctgttc aacttgagcg gcattccgct
     2101 gtgggtctgg ttctcgggca aggtgggcaa gcatcgcgcc tggtgcgtcg gcctggccct
     2161 gttcggcact gcccaatgcg gctatcttct gctgggtccc ggcgacttcc tctacatgct
     2221 tcccatcggc gcgctcaccg ggtttc
//
LOCUS       DBNHOX010000121         1975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_73969, whole genome shotgun sequence.
ACCESSION   DBNHOX010000121 DBNHOX010000000
VERSION     DBNHOX010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_73969"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1361)
                     /gene="ltrA"
                     /locus_tag="AC16UZ_02635"
     CDS             complement(51..1361)
                     /gene="ltrA"
                     /locus_tag="AC16UZ_02635"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HIR6245606.1"
                     /translation="MSLATPEKLRMLQRKLYAKAKSEPSQRFHALYDKVYREDILAHA
                     YALCRNNGGAPGVDGMTFADIEASGAGSWLDGLREELRTKSYRPEAVRRVEIPKPGGG
                     TRPLGIPTIRDRVVQQAAKLVLEPIFEADFDDSAHGYRPRRSAQGAVREVHKALCQGY
                     TEVVDADVSKYFDTIPHDALMKAVARRVSDRWMLKLVKSWLKAPVETREDGGPPRRSG
                     GKKQKRGTPQGGVISPLLANIYINRLLRAWRLNGRDRTFEARLVNYADDFVILSRGRA
                     ESALSWTRHVMERLGLTLNETKTRICDAHHESFDFLGYTFGTERYRRDGHTYLAARPS
                     KKAIGRLKDSIRGDLRPGNMDRMSDIVRRLNQRLRGWSNYFGYGTRYMAYRAIDRFVA
                     TRVRDFLQRRHKGKGRGTRWWPDDVIFEQLGVLRLRTLHLGPRS"
BASE COUNT          285 a          719 c          611 g          360 t
ORIGIN      
        1 gacgtgcggt tttcccgcat ccggctctcc gacgggtttc acgccaaggc tcacgagcgg
       61 ggccccagat gcagcgtgcg caggcgaagg actccgagct gctcgaagat cacgtcgtct
      121 ggccaccacc gggtgccgcg tcctttcccc ttgtggcgcc tctgaaggaa gtcccggaca
      181 cgtgtcgcta cgaaccggtc gatggcccga taggccatgt accgcgtgcc gtacccgaag
      241 tagttggacc acccgcgcag gcgttggttc aggcgtcgca cgatgtccga catcctgtcc
      301 atgttgcccg gccggaggtc gccccggatg ctgtccttga gtcgcccgat ggccttcttg
      361 gacggcctcg ccgccaggta cgtgtgccca tcgcgccgat agcgctcggt cccaaaggtg
      421 tagccgagga agtcgaagga ctcgtggtgg gcgtcgcaga tccgcgtctt ggtctcgttc
      481 agcgtcaagc cgagccgttc catcacgtgg cgtgtccaac tcagcgccga ctccgcgcgt
      541 ccccggctga ggatgacgaa atcgtccgcg tagttgacca gccgcgcttc gaacgtccgg
      601 tctcggccgt tgagccgcca cgcgcgcagc aagcggttga tgtagatgtt ggcaagaagg
      661 ggtgagatca cgccgccctg aggcgtgccg cgcttctgct tcttgccgcc gctccggcga
      721 ggtggccccc cgtcctcccg cgtttcgacg ggggccttca gccagctctt gacgagcttc
      781 agcatccagc ggtcgctgac tcggcgagcc accgccttca tcagcgcgtc gtgcgggatc
      841 gtgtcaaagt acttcgacac gtcggcgtcg accacctccg tatagccctg acacagagcc
      901 ttgtggacct ctcgaaccgc accctgggcc gaccgtcgcg gtcgatagcc atgtgcgctg
      961 tcgtcgaagt cggcctcgaa gatcggttcg aggaccagct tggcggcctg ctgcaccact
     1021 cggtcccgaa tcgtcgggat gcccaggggt cgcgtgccgc ctccgggttt cgggatctcg
     1081 acccgtcgca ccgcttcggg ccgatagctc ttcgtgcgca gttcctccct gagaccgtcg
     1141 agccagctcc ccgccccaga cgcctcgatg tccgcgaacg tcatcccgtc cacgcccggg
     1201 gccccgccat tgttccggca gagcgcatag gcatgcgcca ggatgtcttc ccggtacacc
     1261 ttgtcgtaca gggcgtggaa ccgctgcgac ggctcgctct tcgccttggc gtacagcttc
     1321 cgctggagca tccggagctt ctcgggggtt gccaggctca cgccaatccc cctgcccgcg
     1381 gctccacggc aagcaccccg aagcgaggcc ccttccctcc accggagtta cccggcttcc
     1441 gcagtactat gggcctctcc gactcccacc acgaccatgc cccaagggca cgttgcgggt
     1501 cgcgaccccg cgccgtcgtg ggcctcccac gttgctcaga caaccttcca cacgtgccgc
     1561 ccccactacc ccggagggtc ctcgccgccg cgaacgtcga tcgcttggcg gtgaggcgtc
     1621 ggccttcccc gaatctcagg cgggtcggca ccctcgactg cactttcgag gcctgctcag
     1681 ggttcactcg cgttgcggcc cgcgcggttg cagacccgcc cgaggcgggc cacggtcccc
     1741 ggggcttcga tagcgacagt tgcccatcac caccgcccag gtagctaccg aactggatcg
     1801 acagtggttc gggtaggact ttcacctact ggttgcctgc gccctcgtgg cgcactgaga
     1861 aatgcgggct agggtcgcat ggagggtcgc cgcggtgggc ggcgattcgc cccatctccc
     1921 cgacctcccc ggaagatcat gcgcgcgctg taccgcctcg ccgaccgcct cgccg
//
LOCUS       DBNHOX010000122         2740 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1102587, whole genome shotgun sequence.
ACCESSION   DBNHOX010000122 DBNHOX010000000
VERSION     DBNHOX010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1102587"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="AC16UZ_02640"
     CDS             <1..1224
                     /locus_tag="AC16UZ_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6245607.1"
                     /translation="GRAREPIFYRNPMNPSVTSAVPAKDSMGMDYIPVYADEEAAGPA
                     GTVSIDPIVVQNIGVRTARAQRAALSHTVRALGRIDFDERSIARLHPKVEGWVEEMYV
                     DTTGAQVSEDTMLLSVYSPRLVASQQEYVLALRNYESLNDSPSPDISRGAADLVASSR
                     ARLELLDVPEHQIVELEKSRVPQKSLHIHSPAEGVVLKIGARQGQHVTPMTELYVIAD
                     LSRVWVYADVFEHDLPWVRVGDAVEMTLGALPGETFGGLLDYIYPYAEAKTRTVKARL
                     VFDNPDRLLRPEMFADVLIRTSTEPNALVIPAEAVVLSGLTNQVFVVRAPGRFEPRVV
                     ELGVESEGRVSVLEGLAEGEEVVTSGQFLIDSESKLREAAAKMMARPGDEASSPDGVG
                     DHDHHHGGHDRGASP"
     gene            1221..>2740
                     /locus_tag="AC16UZ_02645"
     CDS             1221..>2740
                     /locus_tag="AC16UZ_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6245608.1"
                     /translation="MIARIIDGAIGERLIVLLGALILAGAGTWAYLTVPVDAIPDLSD
                     VQVIVFTDYPGQAPQVVEDQVTYPLTTAMLAVPKARVVRGYSFFGLSFVYVIFEDGTD
                     MYWARSRVLESLNYVSGRLPAGVNPALGPDATGVGWIYEYALVDRSGRHDLAQLRSLQ
                     DWYLRYPLQTVPGVAEVASVGGFVKQYQVEVDPNALMALGIPLSKIKQAIQRSNNDVG
                     GRLLEISETEYMVRGLGYIRSLEDLGSIPVAADPGGTPIRLGDVARIQIGPELRRGLV
                     DLDGDGEVVGGIVVMRYGENARATIEGVRQRLDELRAGLPEGVEIVTVYDRGDLIDRA
                     VDTLSRSLLIEIVIVSLVVIVFLAHARSAMVAVLILPLGVLIAFLLMRWHGLNANIMS
                     LGGIAITIGAMVDGAIVMVENAHKHMARDAERKGAELSSAERWQAVGAAAREVGPALF
                     FSLLVITVAFLPVFSMQAQEGRLFSPLAFTATYAMAGAAILAITMVPVLMGYLVRGR"
BASE COUNT          453 a          820 c          927 g          540 t
ORIGIN      
        1 gggagggcgc gagaacccat cttctatcgt aaccccatga acccgtccgt cacctctgcg
       61 gtcccggcga aagactccat gggtatggac tacatcccgg tttacgcgga cgaggaggct
      121 gccggcccgg cggggacggt ttccatcgat cccatcgtcg tgcagaacat cggcgtcaga
      181 acggctcgcg ctcagcgcgc cgcgctcagc cacaccgtgc gcgcgttagg ccgtatcgac
      241 ttcgacgagc ggagtattgc caggctgcac cccaaggtcg aaggttgggt cgaggaaatg
      301 tacgtggaca ccaccggtgc tcaggtcagt gaggacacca tgctgttgag cgtgtactcg
      361 ccgcgcctgg tggccagcca gcaggaatac gtgcttgcgc tcaggaacta cgagtcgctc
      421 aacgacagcc ccagtcccga catcagccgc ggcgcggccg atctcgtcgc gagttcccgt
      481 gcccggctcg agctgctgga cgtgccggag catcagatcg tcgagctgga aaaatcccgc
      541 gtcccgcaga agtcgctgca cattcactcg cctgctgaag gcgtggtcct gaagatcggg
      601 gcccgccagg gtcagcacgt gacgcccatg accgagctct acgtgattgc cgatctctcc
      661 cgggtgtggg tttacgccga cgtgttcgag cacgatctgc cctgggttcg ggtgggcgat
      721 gcggtcgaga tgacgctggg agcgctgccg ggagagacat tcggggggtt gctggactac
      781 atatacccct atgcggaagc gaagacccga acggtcaagg cgcgcctggt tttcgacaac
      841 ccggaccggc ttctgagacc ggagatgttc gccgacgtgc tcatccgtac gtcgacggag
      901 cccaacgcgt tggtcattcc cgcggaggcg gtcgtcctct cgggtctgac caatcaggtg
      961 ttcgtcgtgc gtgcgcccgg ccgctttgag ccgcgggtgg tcgagctcgg ggtggagtcc
     1021 gagggtcgag tcagtgttct cgaagggctt gccgagggcg aggaagtggt cacgtccggc
     1081 cagtttctga tcgattcgga atccaagctc cgcgaggctg ctgcgaagat gatggcgcgc
     1141 ccgggtgatg aagcgtcgtc gcccgacgga gtcggcgatc acgaccatca ccacggcggg
     1201 catgaccgtg gagcgtcgcc gtgatcgcgc gcatcatcga tggggcgatc ggcgagcgtt
     1261 taatcgttct gctcggagcg ctcatcctcg ccggtgcggg tacatgggcc tacctcaccg
     1321 tgcccgtgga tgccattccc gacctgtccg acgtgcaggt gatcgtgttt accgactatc
     1381 ccggacaggc gccccaggtc gtggaagatc aggtgaccta cccgctcacc acagcgatgc
     1441 tcgccgttcc caaggcccgc gtggtccgcg gatactcttt cttcggcttg tctttcgtct
     1501 acgtgatatt cgaagacggc accgacatgt actgggcgag atccagagtt ctggagtcgc
     1561 tcaactacgt cagcggccgc ctgccggccg gcgtcaaccc cgcgcttggc cctgatgcga
     1621 cgggcgtggg ctggatatac gagtacgccc tggtggatcg gtccggccgc cacgatctgg
     1681 cgcagctcag gtctcttcag gactggtatc tgcgctatcc gctgcagacc gtgcctggcg
     1741 ttgcggaggt tgcttccgtt ggcgggtttg tcaagcagta ccaggtggag gtggacccca
     1801 acgcgctcat ggcccttggt atcccgctgt cgaaaatcaa gcaggccatt caacgttcga
     1861 acaacgatgt gggcgggcgg ctattggaaa tctccgagac cgagtacatg gtgcggggcc
     1921 tgggctacat tcgatctctg gaggacctcg ggtcgatacc ggtggccgcg gatccgggcg
     1981 gtacgcccat ccgccttggg gatgtggcgc ggattcagat agggcccgag ctcaggcgtg
     2041 ggctggtgga tctggatgga gacggcgaag tggtgggcgg catcgtggtg atgcgttatg
     2101 gggaaaatgc gcgcgccacc atcgaaggcg tgcgccaacg gctggacgag ctcagggccg
     2161 gcctcccgga aggtgtggag atcgtgacgg tctatgaccg cggggatctt atcgatcggg
     2221 cggtggatac cctcagccgg tcgctgctca tcgagatcgt catcgtcagc ttggtggtga
     2281 tcgtctttct ggcccatgca aggtcggcga tggtggccgt cttgatcctc ccgctgggcg
     2341 tgctcattgc tttcctgctg atgcgctggc atggcctgaa cgccaacatc atgtcccttg
     2401 gagggatcgc gatcaccatc ggtgccatgg tggatggggc gatcgtcatg gttgagaacg
     2461 cgcacaaaca catggcccgc gacgccgagc gcaagggtgc cgagctctca tcggccgagc
     2521 gatggcaagc cgtgggcgca gcggctcgcg aggttggtcc ggccctgttc ttctcgctgc
     2581 tcgtgatcac cgtggccttt ctgccggtat tctcgatgca ggcccaggaa gggcggttat
     2641 tcagcccgct ggctttcacg gcgacctacg cgatggcggg cgcagcgatc ctggccatta
     2701 cgatggtacc tgtgctcatg ggctatctgg tcagagggcg
//
LOCUS       DBNHOX010000123         3145 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_652738, whole genome shotgun sequence.
ACCESSION   DBNHOX010000123 DBNHOX010000000
VERSION     DBNHOX010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_652738"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="AC16UZ_02650"
     CDS             complement(<1..988)
                     /locus_tag="AC16UZ_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013729.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIR6245609.1"
                     /translation="MADADQGFAYEFGDWLVEPDLNRIRRGEQEIQLEPSTLEVLCYL
                     LERPGEIVSVDELLDSVWRDSAAEPSAVHSNISRIRRALGESRREPRYIETVSTDGYR
                     AIGAVRRIEAGPHDAELAEALEAVTPPYPAYNGDEPYTFVCYSHQNREQVYQELVRLR
                     SAGINVWYDEGISPGAEWTEEVANAIANCTHLLFFVSTDSVSSKHCLDELSYAQERHK
                     SIVVVYLEPTELPQGLQLSIGRIQALFKYAMRESEYARKLIATLHGAAETRGASTARP
                     TAPRSPAPPSAGVPGRALRRSLSALGIAALIGALGLLVLRGPRIETILRDEPL"
     gene            complement(1165..2715)
                     /locus_tag="AC16UZ_02655"
     CDS             complement(1165..2715)
                     /locus_tag="AC16UZ_02655"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6245610.1"
                     /translation="MTETLTGTNRSAGITYSELLDQDSHPVRDVLRLDSPMEPGPTKV
                     PVERYFSKAFHDLEVEKVWKRVWQMACHQDDIPEVGDYHVYDIAHLSFLIVRTGADEF
                     KAFWNACLHRGRQLKENHGTRARAFRCPFHGWAWNIDGTVLDIPCQWDFPTLDLDELR
                     LPEVQLGRWGGFIFINPDDDNAQSLEDYLGNLSDHFTLLPYERRYKSAHVAKVLRCNW
                     KVAQEAFSEAYHVIATHPTILETIGDANSKYDIFGNYSRAISPAFTPSPHLANMPHYD
                     PLPDARLYKKLRHPLSGYIYERGEDGLVHVIDHQGRISKFTAAGEWVEGEIRNADPNM
                     TDWIGGKQLPDSDDTSGLLPDSLPPDGNLRAMMAQGPRESLRPLLGDRVDEFSDAELL
                     DSIYFTVFPNFHPWGSFNQIVYRFRPHGDNPDECIHECMYFSPVPEGEERPPAAPIHW
                     LGADDDWVDAAELGMLAKVFNQDVVNMPYVQKGLKTMKQPYVIFADYGETKPRHFHKL
                     LNDWLDKP"
     gene            complement(2845..3018)
                     /locus_tag="AC16UZ_02660"
     CDS             complement(2845..3018)
                     /locus_tag="AC16UZ_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245611.1"
                     /translation="MVGLQSYVGELVMYRGFFYEIVRIAVRGDEVRCVAYSDELNREA
                     ELSIADVFLGLAA"
BASE COUNT          555 a          934 c         1043 g          613 t
ORIGIN      
        1 ccaggggctc gtcgcgaaga atcgtctcaa tgcgcgggcc acgcaggacc aacaacccaa
       61 gcgcgccgat cagtgcggcg atgcccaacg cgctcaacga gcggcgcagc gccctccccg
      121 ggactcctgc cgatggcggc gccggggatc gtggtgcggt aggtcgtgcg gtgcttgcgc
      181 cgcgtgtctc ggctgccccg tgcagggtgg cgatcagctt gcgggcgtac tccgactccc
      241 gcatggcgta tttgaacaac gcctggattc tgccaatgga gagctgcagg ccctgaggaa
      301 gctccgtggg ttccaggtag acgaccacga tcgacttatg gcgctcctgg gcatacgaaa
      361 gctcatccag acagtgcttg gatgaaacgg agtctgttga gacgaagaac agcaggtgcg
      421 tgcagttggc gatggcgttg gccacctcct cggtccactc cgcccccggg gagatgccct
      481 cgtcgtacca gacgttgatg ccggccgacc gaaggcgcac gagttcctga tacacctgct
      541 cgcgattctg gtgcgagtag cagacgaagg tgtagggctc gtcaccgttg tacgcagggt
      601 agggcggagt gacggcctcc agggcctcgg cgagctcggc gtcatggggg cccgcttcga
      661 tcctgcgaac tgcgccgatg gcccggtagc cgtccgtgga gacggtttcg atgtacctcg
      721 gctcgcgtct gctttcaccg agggcacggc gaatgcggct gatgttgctg tggacggcgc
      781 tcggttcggc ggcgctgtcg cgccagaccg aatccagcaa ctcgtcgacc gacacgatct
      841 cgcccggacg ttccagcaga tagcagagca cctcgagcgt gctcggctcg agttggatct
      901 cctgctcccc ccgtcgaatt cggttgagat ccggctcgac cagccagtcg ccgaactcgt
      961 aggcgaagcc ctgatcagcg tcggccatag acccgcacgc atcctgccgc ccaacgtcgt
     1021 cgggcctagc ttactccatc atgatgactg ccgcgcggcg gcgccgtggc tctcgggccg
     1081 gtgagtctcg ccacacgcgt accggatcgc gatagcgccg gcgatgggtc gccggcttag
     1141 gcacccgagc acttgccgat cagatcaggg cttatcgagc caatcgttga gcagcttgtg
     1201 aaagtgacgg ggtttggttt ccccgtagtc ggcgaagatc acgtagggct gcttcatggt
     1261 cttcaggccc ttctgaacgt agggcatgtt gaccacgtcc tggttgaaca ccttggccag
     1321 catgccgagc tcggcggcat ccacccagtc gtcgtcggcc cccagccagt gaatgggcgc
     1381 agctggcgga cgctcctcgc cttcgggcac cggagagaag tacatgcact cgtggatgca
     1441 ctcgtcgggg ttgtccccgt ggggccggaa ccggtaaacg atctggttga acgatcccca
     1501 tggatggaag ttgggaaaca cagtgaagta aatggagtcg agaagctcgg cgtcgctgaa
     1561 ctcgtcgacc cgatctccca gtagcggcct gagtgattcg cgcggcccct gggccatcat
     1621 ggcgcgcagg ttgccgtccg gtggaaggga gtcaggcagc agcccgctcg tgtcgtcgct
     1681 gtccgggagc tgtttgcctc cgatccagtc tgtcatgttc gggtccgcgt tacggatctc
     1741 tccttcgacc cactcgccgg cggcggtgaa tttgctgatg cggccctggt gatcgatgac
     1801 gtggacgagc ccgtcctcgc cacgctcgta gatgtagccc gaaagcggat gacgcagctt
     1861 cttgtagaga cgagcgtcgg gcagcggatc gtagtgcggc atgttcgcca gatgcgggct
     1921 cggcgtgaag gcgggagaga tcgctcgtga gtagttgccg aagatgtcgt acttcgagtt
     1981 cgcgtcgccg atggtctcga gaatcgtcgg gtgggtggcg atgacgtgat aggcctcgct
     2041 gaacgcctcc tgagccacct tccagttgca acgcagcacc tttgctacgt gggcagactt
     2101 gtaccgccgc tcgtagggga gcagcgtgaa gtgatcggag aggttgccga ggtagtcttc
     2161 cagagactgg gcgttgtcat cgtccgggtt gatgaagatg aaaccgcccc agcggcccag
     2221 ctgcacctcg ggcagtctca gctcatccag atcgagagtc ggaaaatccc actggcaggg
     2281 gatgtccagt accgtgccgt cgatgttcca ggcccagccg tggaacggac atcgaaacgc
     2341 gcgcgctcgt gtgccgtgat tctccttgag ctgccggccg cggtgaaggc aggcgttcca
     2401 gaacgctttg aactcgtcgg caccggtgcg gacgatgaga aatgagagat gggcgatgtc
     2461 gtatacgtgg tagtcgccga cctccggaat gtcgtcctga tgacaggcca tctgccagac
     2521 ccgcttccag actttttcga cctccaggtc gtggaaggct ttggagaaat atcgctccac
     2581 cggtaccttc gttgggcccg gctccatggg cgaatcgagc cgcaggacgt cgcgcaccgg
     2641 atggctgtcc tggtcgagca gctcgctgta ggtgatgccc gcggagcggt tggtgccagt
     2701 cagtgtttcg gtcatggatg gatccccgac tcttgcgcag cgggccatgc tggtatcgcc
     2761 ccgtagaacc cgcaatacgg gatacccgag gggtccggca taaaggcctg gtgccgccgg
     2821 tcgagaggag gaggcgcagg tgggttaggc cgcgagaccg aggaacacgt cggctatgga
     2881 gagttcggct tccctgttga gctcgtcact gtacgcaacg cagcggacct cgtcaccgcg
     2941 gacggcgatg cgcacgatct cgtagaaaaa gccgcggtac atgaccagct cgcctacgta
     3001 gctctgcaat ccgaccatcg gtggttatcc agaaaatgac ccgaatattg tgatccgttt
     3061 ctcggtttgt ggtgtgagta agcgtaagcg gtatgtcagt tggatatccg gaagtcgttg
     3121 gcgcgggcac cgcattggtc ccctt
//
LOCUS       DBNHOX010000124         6258 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_202558, whole genome shotgun sequence.
ACCESSION   DBNHOX010000124 DBNHOX010000000
VERSION     DBNHOX010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_202558"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="AC16UZ_02665"
     CDS             <1..655
                     /locus_tag="AC16UZ_02665"
                     /inference="COORDINATES: protein motif:HMM:NF018038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR6245612.1"
                     /translation="FSNESAIASADLLVSYTCDVRPSPDQQRWLKSFLERGGRWFALH
                     GTNSLLDFDTTDGPIEAFGVTIPGMPFAPDLTPDFMRLLGSRFVTHPPIQKFRVHVSA
                     PDHPLVTGLDDFETEDEPYCCDFYDEVEVLLEARYSSEWMTELPAEWDDPGTPRPQMY
                     LRRLGKGEVLYLTLGHCCGKFDMQPIMEECDVVRCSWDRPVYYELLRRGIRWGSRTA"
     gene            848..2146
                     /locus_tag="AC16UZ_02670"
     CDS             848..2146
                     /locus_tag="AC16UZ_02670"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014579434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6245613.1"
                     /translation="MSEAHDVEPTSESTMLNKINPAELPYQDPWTTPLEELDVARPQL
                     FHADAQWPYFERLRKEAPVNYCNFSAFGPYWSVTSYDMIKAVDQNHEVFSSEPGITIE
                     DQDEDFQLPMFIAMDPPKHDVQRKAVQTAVAPRNLLEFESIIRERTASVLDGLPENED
                     FDWVEHVSINLTTMMLATLFDFPFEDRQKLTYWSDVVTGGVYTEFTQESQDAARADLL
                     QCLAYFTELWQERAKRPGGFDLISMLAHHPATKDLLTRPMEYLGNLVLLIVGGNDTTR
                     NSMSGGVYALNRFGDEYDKLRANENLIPNMVSEIIRWQTPLAHMRRRAKQDVELGGET
                     ISAGDKVVMWYVSGNRDESVFEEADRLIIDRANARQHLSFGYGIHRCMGNRLGEMQLR
                     ILWEEIQKRFSFVEVTGDPERSFSAFVKGYTKLPVRVHRR"
     gene            2156..2785
                     /locus_tag="AC16UZ_02675"
     CDS             2156..2785
                     /locus_tag="AC16UZ_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6245614.1"
                     /translation="MSLQAYRDARAREKRADILSAASRLFAERGFAQVSTAALAEEAG
                     VSTATLYRYFADKESLFVAVIDSLVADVIQQMADLPRASDDRLRELAFRYANLLCDPI
                     VVGIIRSVVADRQSTSAFRDSLESHGNEIFANEFDVEIRALLSAHDSDELDLAHQASI
                     ELRGALEHVTLLPELLFHEHLAEEELKGVVEQTLISWRRRWLGEQGSST"
     gene            complement(2803..3243)
                     /locus_tag="AC16UZ_02680"
     CDS             complement(2803..3243)
                     /locus_tag="AC16UZ_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6245615.1"
                     /translation="MTSVANQEPIAEGLFTWPSNDPRLLGSRCDDCGVVTFPAQNACP
                     ACCGEDTTTIELERRGTLWTWTIQGFLPNRPPYDGPETPETFEPFGVGYVELPGQVRV
                     EARIKTDDLSKLKIGMEMELVVEKYVERDGKDIMAYFFKPADEL"
     gene            complement(3323..4456)
                     /locus_tag="AC16UZ_02685"
     CDS             complement(3323..4456)
                     /locus_tag="AC16UZ_02685"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR6245616.1"
                     /translation="MLRRVLATVFLCTALPLCHAAMTAADLGRLLFFDANLSLQRTQS
                     CASCHDESRAFSDGRDNGVRGAASLGDDGRSLGDRNAPALTYVALTPPFRIRGEGRPA
                     GGFFLDGRVATLQEQAAEPFVNPLEMQMPDPTAVVARVRENPTYERSLRRLFGEAVFE
                     DTDTAYRAVGESLAAFEQADELASFDSRYDRHLRGEYQMSEQEALGRDLFFSQLTNCS
                     LCHALSPHATNDARETFTDYGFRNIGVPANAHLRSANGTKPGHTDRGLLENPAVERAE
                     HAGKFKVPTLRNVAVTAPYMHNGVFRELRTAIHFYNRYLVRNPASMTNPETGEPWAPA
                     EVEETVDLALLREGQPLSDERIALIEAFLRTLTDERYEHLLEK"
     gene            complement(4486..5898)
                     /locus_tag="AC16UZ_02690"
     CDS             complement(4486..5898)
                     /locus_tag="AC16UZ_02690"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6245617.1"
                     /translation="MAKPFPKHPHLEGGFAPIHMECDAPDLVVEGEVPRELNGVFYRN
                     GPNAQFAPRGDYHLFAGDGMIHGFYIRNGQVAYKNRWVRTVKWTREHEAGGALFSAFN
                     PMDSDESVRGMETDGLANTNVVWHGGKLLALEEAHAPFELDPYTLESKGAWRYDGKLE
                     GPMTAHPKIDCETGEMLMFGYNAKGLISEDMAFHVVDGDGNLTRSEWFKAPYASMVHD
                     FMITRDHVIFPIMPLTGSMERAMQGGPVYAWEPDKGVHIGIMPRNGSVDELRWFKGDP
                     SFVFHPMNAHSDGNMVTCDVCEFEEAPLFPAPDGTPGDPDKAVPRLTRWTFDLGSNTD
                     DYSSERLDDLACEFPRLDERFTGLGYRHGYFACDLKPEFQVGAFNGIGHVDHATGKID
                     VYAVGQACATNEPIFVPRTEDSAEGDGFLLANVYDAERKASHLVILDAQNVSAGPLAK
                     AYLDHRVPFGFHGNWMPGQA"
     gene            6038..>6258
                     /locus_tag="AC16UZ_02695"
     CDS             6038..>6258
                     /locus_tag="AC16UZ_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020799109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR6245618.1"
                     /translation="MSNEALQNAYETLSARIGKSSEPTDWFEITQERINSFADATMDH
                     QWIHIDPERAAAGPFGTTIAHGQLTQSIM"
BASE COUNT         1089 a         1940 c         2043 g         1186 t
ORIGIN      
        1 tttcagcaac gaaagcgcga tcgcgtctgc cgatctgctg gtcagctaca cctgtgatgt
       61 gcgtcccagc cccgaccagc agcgttggct gaaatccttt ctcgaaagag gaggacgatg
      121 gttcgcgctg cacgggacca actcgcttct cgatttcgat accaccgatg gtcccatcga
      181 ggcgtttggg gtgaccattc ccgggatgcc gttcgcgccg gatctcaccc ctgacttcat
      241 gcgtctgctg ggcagtcgat tcgtcaccca tccgccgatt cagaagtttc gcgtgcacgt
      301 cagcgcgccg gatcatccgc tcgtgaccgg gctggacgac ttcgagacgg aggacgaacc
      361 ctattgctgc gacttctacg acgaggttga ggtgctgctg gaagcccgct actcgagcga
      421 gtggatgacg gagctgcctg cggagtggga cgatcccggt accccgcggc cccagatgta
      481 cttacggcgc ctggggaagg gggaggtcct ttacctgacg ctcgggcact gctgcggtaa
      541 gttcgacatg cagccgatca tggaagagtg cgacgtcgtc cgctgcagtt gggaccgccc
      601 ggtctactac gagcttctcc ggcgcggcat tcgctgggga agccggacgg cttgagtgtc
      661 ctgagcacgc gcgatacctc cttggtggtg cagatctgat gcccggctcc ttggattctg
      721 tgtacctggt ggcggttttt ctgatggtaa ttgtaaactg accagtttag tttagaattc
      781 acgttttcgg ccgcgggcca accgtgcatt gcccgcgtca gtcagacaaa cgctttcagg
      841 agtcctcatg agcgaagccc acgacgtcga accgacctcc gagtccacca tgctgaacaa
      901 gatcaacccg gcggagctgc cttatcagga tccgtggacc acgccgctcg aagagctgga
      961 cgtggcccgt ccccagctgt ttcacgcaga tgcccaatgg ccctacttcg agcgtctgcg
     1021 caaggaagcg cccgtcaact actgcaattt cagcgccttc gggccgtact ggtcggtgac
     1081 gagctacgac atgatcaagg cggtggatca gaaccacgag gtgttcagct cggagcctgg
     1141 gatcaccatc gaggatcagg acgaggactt ccagctgccc atgttcatcg ccatggaccc
     1201 gcccaagcac gacgtgcagc gcaaggccgt ccagaccgcc gtggcgccgc gcaacctgct
     1261 ggaattcgag tccatcatcc gtgagcgcac ggccagcgtg ctggacgggc tgccagagaa
     1321 cgaggacttc gactgggtcg agcacgtctc catcaacctc accaccatga tgctggcgac
     1381 gctgttcgac tttcctttcg aggatcgtca gaagctgacc tactggtctg acgtggtgac
     1441 gggcggcgtg tataccgagt tcacccagga atcccaggat gcggcccggg ccgacctgct
     1501 gcagtgcctg gcctacttca ccgagctgtg gcaggagcgg gccaagcggc ctggtggttt
     1561 cgacctcatc tcgatgctgg cccaccaccc ggccaccaag gatctgctca cccgtcccat
     1621 ggagtatctg ggcaatctgg tgctgctgat cgtcggcggc aacgacacca cgcgaaactc
     1681 catgagcggc ggcgtttacg ccctgaacag gttcggtgac gagtacgaca agctgcgggc
     1741 caacgagaac ctcatcccga acatggtctc ggagatcatc cgctggcaga cgccgctggc
     1801 ccacatgcgt cggcgggcca agcaggacgt ggagctaggt ggggagacca tctccgccgg
     1861 ggacaaggtg gtcatgtggt acgtgtccgg caatcgggac gagagcgtat tcgaggaggc
     1921 cgaccgcctc atcatcgatc gcgcgaatgc ccgtcagcat ctctccttcg gctatggcat
     1981 tcatcgctgc atgggcaatc gtctgggcga gatgcagctg cgcatcctct gggaagagat
     2041 ccagaagcgg ttctccttcg tggaggtcac cggcgacccg gaacgctcct tctccgcgtt
     2101 cgtcaaggga tacacgaagc tgccggtgcg cgtgcatcgg cgctgatcac tcgccatgtc
     2161 tctgcaagcc tatcgtgacg cgcgggcccg cgagaagcgg gccgacatcc tgagcgccgc
     2221 gtcgcgcctg tttgctgagc gcggcttcgc acaggtgtcc acggcagcgc tggccgagga
     2281 agcgggcgtg tcgacggcga cgctatatcg ttacttcgca gacaaagagt ctctgttcgt
     2341 tgccgtcatc gactccctgg tggccgacgt gatccagcag atggccgacc tgccgcgcgc
     2401 gtccgatgac cggcttcgtg agctcgcgtt ccgctatgcg aatctgctct gcgaccccat
     2461 cgtcgtcggc atcatacggt ccgttgtggc cgatcggcag agcaccagcg cgttccgtga
     2521 cagtctggag agccatggca acgagatatt cgccaacgag ttcgacgtcg agatcagagc
     2581 gctgctgagc gcccatgaca gcgacgagct ggatctggcg catcaggcga gcatcgagct
     2641 gcggggcgcg ctcgagcacg tgacactcct gcctgagctg ctgttccacg agcacctggc
     2701 cgaagaagag ctcaaaggag tggtcgagca gacgctgatt tcctggcgca ggcgctggct
     2761 cggagagcag ggctcttcaa cctgactgaa gaagcgggtg gtctagagct cgtccgccgg
     2821 cttgaagaag taagccatga tgtccttgcc gtcgcgctcg acgtatttct cgaccacgag
     2881 ctccatctcc atgccgatct tcagcttgga gagatcgtcc gttttgatgc gtgcctccac
     2941 ccgcacctgg ccgggcagct cgacgtagcc cactccgaac ggctcgaagg tttccggcgt
     3001 ctcggggccg tcgtagggtg ggcggttggg aagaaagccc tgaatggtcc aggtccacag
     3061 ggtgccccga cgctcgagct caatcgtcgt cgtgtcctca ccgcagcagg cggggcaggc
     3121 attctgcgcg gggaaggtca ccacgccgca atcgtcgcag cggctcccga gcaggcgcgg
     3181 atcgttcgat ggccaggtga acagcccctc ggcgatgggt tcctggttgg caacactggt
     3241 cacttaagct ttcccgaaag atgtgaacat ggtttgtagc agagccggag ggcgtttgtc
     3301 accgggtggc cgggaagcgg gctcatttct cgagcaggtg ctcgtatcgc tcgtcggtca
     3361 gcgtgcgcag aaacgcttcg atgagcgcaa tacgctcatc agatagcggc tggccctcgc
     3421 gaagcagggc caggtcgacg gtttcctcca cctcggctgg cgcccacggt tcgccggtct
     3481 ccggattggt catgctcgcg gggttgcgca ccagatagcg gttgtagaaa tgaatggccg
     3541 tgcggagctc gcgaaacacc ccgttgtgca tgtacggggc cgtcaccgcc acattgcgta
     3601 gagtgggcac cttgaacttg ccggcgtgct ccgcgcgttc gacggccggg ttttcgagca
     3661 ggccgcggtc cgtgtgtccg ggtttcgtgc cgttcgcgct gcgcaggtgg gcgttggccg
     3721 gcacgccgat gttgcggaac ccgtagtccg tgaacgtctc ccgcgcgtcg ttggtggcgt
     3781 gggggctgag cgcgtggcag aggctgcagt tggttagctg agagaagaac agatcccggc
     3841 cgagggcctc ctgctcgctc atctgatact cgccccgcag atgccggtcg tagcgggaat
     3901 cgaagctggc gagctcgtcc gcctgctcga aggcggcgag gctttcgcca acggcgcggt
     3961 aggccgtgtc ggtgtcctcg aataccgcct ctccgaacag ccgccgcaag gaacgctcgt
     4021 aggtcgggtt ttcccgcacc cgggccacca cggcggtggg atcgggcatc tgcatctcca
     4081 gcgggttcac gaacggctcc gcggcctgtt cctgcagggt ggcgacgcgc ccgtcgagga
     4141 agaagccgcc cgctggtcgg ccctcgccgc ggatccggaa cggcggcgtc aaggcgacgt
     4201 aggtcagtgc tggggcgttc cggtcgccca aagagcgccc gtcgtctccc agggaggccg
     4261 cgcctctcac gccgttgtcg cgaccgtcgg aaaacgcccg ggactcgtca tggcagctgg
     4321 cgcaggactg ggtccgctgc agtgagaggt tggcgtcgaa gaacagcagt cggccgagat
     4381 cggcggccgt catcgccgcg tggcagagcg gcaacgctgt gcacaggaaa actgtcgcca
     4441 gcacgcgcct gagcatcgag ccttccgggt cgggccgagc cgtgctcagg cctggcccgg
     4501 catccagttg ccgtggaagc cgaagggtac ccggtgatcc aggtaggcct tggccagcgg
     4561 cccggcgctc acgttctggg catcgagaat cacgaggtgg ctggctttgc gctcagcgtc
     4621 gtagacgttg gcgagcagaa agccgtcacc ctccgccgag tcctcggtgc ggggcacgaa
     4681 gatcggctcg ttggtcgcgc aggcctgccc cacggcgtag acgtcgatct tgcccgtggc
     4741 gtggtccaca tggccgatac cgttgaacgc gccaacctgg aactcgggct tcaggtcgca
     4801 ggcgaagtag ccgtgccggt agccgagccc ggtgaagcgc tcatccagtc gcgggaactc
     4861 gcaggcgaga tcgtcgagcc gctcgcttga gtagtcgtcg gtgttgctgc ccaggtcgaa
     4921 cgtccatcgg gtgagccggg ggactgcctt gtcggggtcg cccggggtgc cgtcgggtgc
     4981 cgggaagaga ggggcctcct cgaactcgca gacgtcgcag gtcaccatgt tgccgtcgct
     5041 gtgcgcgttc atggggtgaa agacgaacga gggatccccc ttgaaccagc gcagctcatc
     5101 cacgctgccg ttgcgcggca tgatgccgat gtggacgccc ttgtctggct cccaggcgta
     5161 gacgggtccc ccctgcatgg cccgctccat ggatccggtc aggggcatga tggggaagat
     5221 cacgtgatcc cgggtgatca tgaagtcgtg aaccatggac gcgtagggcg ctttgaacca
     5281 ctcggagcgg gtgaggtttc cgtcgccgtc caccacgtgg aaggccatgt cctcggagat
     5341 gaggcccttg gcgttgtagc cgaacatgag catctcaccg gtctcgcagt cgatcttggg
     5401 gtgcgccgtc atcgggcctt ccagcttgcc gtcgtagcgc caggctccct tcgattccag
     5461 ggtgtaagga tccagctcga acggagcgtg tgcctcctcg agggccagca gcttgccgcc
     5521 atgccacacc acgttggtgt ttgccaggcc gtcggtctcc atgccgcgta cgctctcgtc
     5581 ggagtccatg gggttgaacg cgctgaagag cgccccgccg gcttcgtgtt ccctggtcca
     5641 cttgacggta cgcacccagc ggttcttgta cgccacctgg ccattgcgaa tgtagaagcc
     5701 gtggatcatg ccgtccccgg caaagaggtg gtaatcgccg cgcggcgcga attgagcgtt
     5761 tggcccgttg cgatagaaga cgccgttcag ctcgcgtggc acctcgccct cgacgaccag
     5821 atcgggggca tcacactcca tgtgaatggg ggcaaagccg ccttccaggt gaggatgctt
     5881 cgggaatggt ttagccatgg gccacctcct gtgtttgctc aactatagag ggtttgacgc
     5941 cctttgcgcg agcgatgtcc gggctctgct cgggcgcgca tgacatgatt cggcgcggcg
     6001 tagataatcg gcgctctttc aacctggggg aaatcacatg tcgaacgaag ctctgcagaa
     6061 tgcttacgag acgctaagtg cccgaatcgg caagtcttca gaacctaccg actggttcga
     6121 gatcacgcag gaacgcatca actcgttcgc cgacgccacc atggatcacc agtggatcca
     6181 catcgacccc gaacgtgcgg cggcgggccc gtttggcacc acgatcgctc acggccagct
     6241 gacgcagtcg atcatgag
//
LOCUS       DBNHOX010000125         9626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_202626, whole genome shotgun sequence.
ACCESSION   DBNHOX010000125 DBNHOX010000000
VERSION     DBNHOX010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_202626"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /gene="rsgA"
                     /locus_tag="AC16UZ_02700"
     CDS             complement(<1..459)
                     /gene="rsgA"
                     /locus_tag="AC16UZ_02700"
                     /inference="COORDINATES: protein motif:HMM:NF015170.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HIR6245619.1"
                     /translation="MNGARPTLPELGWTPFFAEQLSPEEADRLIPARVFGVQRTHITV
                     MAEEGELSMPLGGRWFQLDPQDRPTVGDWVLVSEQRDSVVRLLERRSLLKRLSPGRAG
                     AVQLIAANVDTLFLVTSCNQDFNPRRLERYLALAYESGIEPVVVLTKADLC"
     gene            complement(466..1221)
                     /locus_tag="AC16UZ_02705"
     CDS             complement(466..1221)
                     /locus_tag="AC16UZ_02705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6245620.1"
                     /translation="MTIRLEIDGIEVELRRNSRRRTRIGMAFDPSGFVIMDAPPNVGE
                     NEVREVVAEHHRWLRVRLDKARDAVHEVRALNYVAGELVHYLGQAYRLEVRAGGEVRV
                     ELSAPAHSQLPLFADVAFGTLQVTTPEQSPDGVKTAVCDWYSAQGDRVFAERLAGWSE
                     LPWLNGTTPRWRHTFMRSQWGSCSARGKISLNTHLVKAPVSLVDYVILHELCHLEHHD
                     HGRRFYALMERHMPDWERRRAALDRFLPVLLHD"
     gene            complement(1218..2438)
                     /locus_tag="AC16UZ_02710"
     CDS             complement(1218..2438)
                     /locus_tag="AC16UZ_02710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6245621.1"
                     /translation="MTIRSYDEDRDLKAIERIWFECGWLENSRQAAYLKDFLAAGDCL
                     TATIDDTAECAVHVSPGALQYVDEQLELCAVTAVTTSRVARKQGFARELTARQLARGA
                     DDGAEIAALGMFEQGFYDQLGFGTGSYEHEFTFDPATLTVDVPFRPPSRLDRGNWRDI
                     HASLAKRLRGHGGCVLRPSYLIKAELGFSEDGFGLGYFDGSDLTHFFWGEPRGEHGPY
                     RIHFLAYRDAEQLMELLALMRSLGDQVSSIVLLEPPEVQLQDLLDRPFRARRNTRASA
                     HANQHRSIAFWQARVLDVEACVAVRHWPGDEVDFNLVLTDPLSEILAGEAWSGVAGEY
                     VVHVGADSSARSGFEAGLPVLHAGVGAFTRLWLGVVGASALALSDELTGPTELLGRLD
                     EALCLPPARTTWDF"
     gene            complement(2473..3429)
                     /locus_tag="AC16UZ_02715"
     CDS             complement(2473..3429)
                     /locus_tag="AC16UZ_02715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6245622.1"
                     /translation="MELDYSNIDPELRPALDFLPAELDLITRETLHATRAVLAAAPAP
                     DVETDVEVTEQIIAGPGGDLRLLIYRSPVAEGGPGVLWLHGGGYILGAVDDDRARLVA
                     ESLGCTAVSVDYRLAPEHPFPAAVEDAYAALCWLAGNAGALGVDAERLSIAGLSAGAG
                     LTAGLALLNRDRGGPAVAFQLLLSPMLDNLHDTASGRIVNHPTWSRQTSLNAWEMYLD
                     GTPGLDASPYAAPSRATDLSGLPEAYVSVGAEDLFRDECIDYARRLHEHGVATELAVF
                     PGMYHVAENFAPQARVCQRLHGSYLRALADGLGVSRGAADGS"
     gene            complement(3520..4272)
                     /locus_tag="AC16UZ_02720"
     CDS             complement(3520..4272)
                     /locus_tag="AC16UZ_02720"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6245623.1"
                     /translation="MSQVQPIRPAATVILVRDAADGFEILMLKRTSGAAFAGGMFVFP
                     GGRVDGDDHLHRYDEHRVGPSDVQQAQQRALGAEWRGYWIAGIRESFEEAGLLLAYDR
                     AGELLAYDDDAVEERFQSYRGPLHAGQVSLETICRQEGLRLAVDRIHFHSRFVTPLGR
                     PRRFDTRFFIAEAPPGQTGRHDDKETVDSIWISPGEALARHAVEEFGLMGVTKLQLEG
                     LAGHRSKADVLAAAARRADFPTRRPVLPPSER"
     gene            4367..5281
                     /locus_tag="AC16UZ_02725"
     CDS             4367..5281
                     /locus_tag="AC16UZ_02725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6245624.1"
                     /translation="MKLSVEFPSVAYREGPEGIIRLAKAIEDIGYDQLDMFDHVVMGY
                     PTETRAAPIYPPQMPIMEALMVLAYAAAVTTRIGLGTEVLVLPQRQPVLVAKQISTLD
                     TLSGGRVRLGVGVGWQASEYDALEESFANRGRRMDEAIRLLRAYWSEEKVEFAGEYYT
                     SHAMAMEPKPPQGGQLPIWIGGLAPAALRRVGELGDGWLATAFTDPDRARAAVATIHR
                     HAEDAGRDPAAIGLQQMLDVPPRDDSGKGFYADLDQVEARALAVKEMGFEWGALNATA
                     IFQSGARSVDAMIDTLGALHERLRAATA"
     gene            5329..5595
                     /locus_tag="AC16UZ_02730"
     CDS             5329..5595
                     /locus_tag="AC16UZ_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HIR6245625.1"
                     /translation="MSSQRDDHAYWRANLKLGAVLLGIWFVVSFGFGILLVEPLNRIP
                     LFGFKLGFWWAQQGAIFVFVILIFVYSRLMKRIDRRYGVHDDED"
     gene            5611..7383
                     /locus_tag="AC16UZ_02735"
     CDS             5611..7383
                     /locus_tag="AC16UZ_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR6245626.1"
                     /translation="MDVQLLTYLFVGLSFALYIGIAIWSRAASTREFYVAGGHVHPVA
                     NGMATAADWMSAASFISMAGIIAFMGYDGTVYLLGWTGGYVLLALLLAPYLRKFGEFT
                     VPDFIGTRYYSNTARVVAVVSLIVISFTYVAGQMRGVGIVFSHFLGVEIELGVVIGMA
                     VVFMYAVLGGMKGITYTQVAQYCVLIFAYLVPAIFISMLITGIPIPQIGLGAAVNDGS
                     GVSVLARLDATLVELGFGPYTQGSKSTIDVFAITMALMIGTAGLPHVIVRFFTVPKVS
                     DARKSAGYALLFIALLYTTAPAVGAFARLNFVETVHGTGYADVAGWFKSWEDVGLIGW
                     LDKNEDGAIQYAPGAPFEGKPEFDGVRGPDGQRLIRNAPTGNANEVYVDRDIMVLANP
                     EIAALPPWVIALVAAGGLAAALSTAAGLLLVISTSVSHDLIKKTMARGISDRQELLYA
                     RIAAALAIAVAGYLGINPPGFVAQVVAFAFGLAAASLFPAILLGIFSKRMNREGAIAG
                     MVVGLTFTFAYIVYFKFVAPGDNNAAHWWLGISPEGIGMVGALANFATAAIVSRFASP
                     APEDVQQLVEKIRIPRGVETPHTH"
     gene            complement(7416..9608)
                     /locus_tag="AC16UZ_02740"
     CDS             complement(7416..9608)
                     /locus_tag="AC16UZ_02740"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HIR6245627.1"
                     /translation="MSATKRVSRGKLSVAAELDDLVSQRIAPGTGVDAEAFWAGFESI
                     VDDLAPRNRALLARRDELQATIDEWHEDRRGRPLDAQEYRAFLEQIGYLVPEPAPFKI
                     LTADVDDEIARIAGPQLVVPVMNARYALNAANARWGSLYDALYGTDVLPEEPGREKGT
                     SYNPARGELVVERAARFLDETVPLAGASHSEVIAYGIGDGPGGRRLRAILDNGGNTGL
                     ENPEQFVGFIDEDDPSTLLLVHNGLHIEIQIDRSHPVGAAHPAGVKDVVLESAVTTIQ
                     DCEDSVAAVDAEDKCVVYGNWLGLMRGDLEEVMDKGGRAVTRRLNPDRTYVGLDGQPL
                     TLHGRSLMLVRNVGHLMTTDAVLDGAGNEIPEGILDAAVTAFAAMHDLLGTGSVRNSR
                     AGSVYIVKPKMHGPEEVAFTCDLFSRVEQLLGLGPNTLKVGIMDEERRTTVNLRACIF
                     EARERVVFINTGFLDRTGDEIHTSMQAGPVLPKEAIKAEPWIQAYEDANVDAGLAAGF
                     SGVAQIGKGMWPKPDEMKEMLAAKIAHPQAGANTAWVPSPTAATLHATHYHQVSVFER
                     QQALAAREPASLDDILRIPLLGGRNLSAEEVQAELDNNAQGILGYVVRWIDHGVGCSK
                     VPDINDVGLMEDRATLRISSQHIANWLHHGICSREQVLGTMQRMAAVVDGQNAGDAAY
                     RNMAPDFDASVAFQAACDLVFKGLEQPNGYTEPVLHARRREAKARFGS"
BASE COUNT         1587 a         3316 c         3144 g         1579 t
ORIGIN      
        1 gcatagatcc gccttggtga gcaccaccac gggctcgatg cccgactcgt atgccagcgc
       61 caggtaacgc tcgaggcggc gcggattgaa atcctgattg cacgaggtga ccaggaacag
      121 cgtatcgacg ttggccgcga tgagctgcac cgcacccgcg cggcccgggc tcaatcgctt
      181 gaggaggctt ctgcgttcga gcagcctcac cacgctgtcg cgctgctcag aaaccagcac
      241 ccagtcgccc accgtgggcc ggtcctgggg atcgagctga aaccagcgcc cgcccagggg
      301 catggaaagc tcaccctcct cggccatcac cgtgatgtga gtccgctgca cgccgaaaac
      361 ccgggcgggg atgagccggt ctgcctcctc gggagacagc tgctcggcga agaacggtgt
      421 ccagccgagc tcggggagag tgggcctggc gccgttcacg agagatcagt catggagcag
      481 caccggcagg aagcggtcca gggcggcgcg gcggcgctcc cagtcgggca tgtgccgctc
      541 catcaacgcg taaaagcgcc gtccgtgatc gtgatgctcg agatggcaca gctcgtgaag
      601 aatcacgtag tcgacgagag acacgggggc cttgaccaga tgggtgttga gcgagatctt
      661 gccccgcgcg ctgcagctgc cccactggct gcgcatgaag gtgtgccgcc agcgcggcgt
      721 cgttccgttg agccaaggaa gttccgacca ccccgccagc cgttcggcga agacccgatc
      781 gccctgtgcc gagtaccagt cgcacacggc ggttttgacc ccgtccggcg actgctcggg
      841 cgtcgtcacc tgcagggtac cgaacgccac gtcggcaaac agcggcagct gggaatgggc
      901 aggcgcgctg agctccaccc gcacctcccc cccggcgcga acctcgagcc ggtacgcctg
      961 gccgagatag tggacgagct cgccggcaac atagttgagc gcgcggactt cgtgaaccgc
     1021 gtcgcgcgcc ttgtcgagcc tgacgcgcag ccagcgatga tgctcggcga ccacctcccg
     1081 gacttcgttc tcgccgacgt tggggggcgc gtccatgatc acgaatccgc tggggtcgaa
     1141 agccatgccg atacgggtgc gacgccggga gttgcggcgc agctccacct cgatgccgtc
     1201 gatttccagc cgaatcgtca aaagtcccag gtcgttctgg ccggcggcag gcagagagcc
     1261 tcgtccagcc ggccgagcag ctcggtcggg cccgtcagct cgtcgctcag agcaagagcc
     1321 gaagcgccga ccaccccgag ccacaatctc gtaaacgcac cgacgcccgc atgcagcacc
     1381 ggcagcccgg cctcgaagcc cgatcgggca gaggagtcgg cgcccacgtg caccacgtac
     1441 tcacccgcca cgcccgacca ggcttctcct gccaggatct cgctcaaggg gtcggtgagc
     1501 acgaggttga agtcgacctc gtcgcccggc cagtgccgaa ccgccacgca ggcctcaacg
     1561 tccagcaccc gcgcctgcca gaacgcaatg gaccggtgct ggttggcatg ggcggaagcc
     1621 ctggtgttgc ggcgcgcgcg aaatggccga tccaggagat cctgcagctg cacttcaggt
     1681 ggctccagca ggacgatgga ggacacctga tcaccgaggg agcgcatcag cgccagaagc
     1741 tccatgagct gctcggcgtc gcggtaggcc aggaagtgta tgcgataggg tccgtgctcg
     1801 ccgcggggtt cgccccagaa gaagtgggtg agatcggatc cgtcgaagta cccgaggccg
     1861 aacccgtctt ccgagaagcc gagctccgcc ttgatcagat acgaagggcg cagcacgcag
     1921 ccgccgtggc cgcgaagcct cttcgccagg gaggcatgaa tgtcgcgcca gttgcctcga
     1981 tcgagccggc tcgggggtcg aaacggcacg tctaccgtca gggtcgccgg gtcgaacgta
     2041 aactcgtgct cgtagctgcc cgtgccgaac ccgagctggt cgtaaaagcc ctgctcgaac
     2101 atgccgagcg ccgcgatctc ggcaccatcg tccgctccgc gggccagctg gcgcgccgtg
     2161 agctcgcggg caaacccctg cttgcgcgcg acccggctgg tagtcacggc ggtcaccgcg
     2221 cacagctcga gctgctcgtc gacgtactgc aaggcgccgg gggaaacatg caccgcgcac
     2281 tccgctgtat cgtcgatggt ggcggtgagg cagtcgcccg cggcgaggaa gtccttgaga
     2341 taagcagcct gacgcgaatt ttccagccac ccgcactcga accagatgcg ctcgatggct
     2401 ttgaggtccc ggtcttcgtc gtagctgcgg atcgtcatag gcacgcaggg cgcggaaaac
     2461 ccggattatg cgtcaggagc cgtcggccgc gccacgcgac acccccagcc cgtccgccag
     2521 ggcgcgcaga tagcttccgt gcagccgctg acagacccgc gcctggggcg cgaagttttc
     2581 agccacatgg tacatgccgg gaaacacggc cagctccgtg gccaccccgt gctcgtggag
     2641 ccggcgggca tagtcgatgc actcgtcccg gaacagatct tccgcgccaa cggatacgta
     2701 cgcctcaggg agaccggaga ggtcggttgc gcggctggga gccgcatagg gtgaggcgtc
     2761 cagcccgggc gtgccgtcca ggtacatctc ccaggcgttc agcgacgtct gcctggacca
     2821 ggtcgggtgg ttgacgatcc ggcccgaggc ggtgtcgtgg aggttgtcga gcatggggga
     2881 aagcagaagc tgaaaggcga ccgccggccc tccccggtcg cgattgagca gggcaagccc
     2941 cgccgtcagc cccgcccctg cactgagccc ggcaatggaa aggcgctcgg cgtccacacc
     3001 gagcgcgccg gcgttcccgg ccagccagca caacgcagcg taggcatctt caaccgccgc
     3061 cgggaaagga tgctccggcg ccagacggta atctacggat accgccgtgc acccgagcga
     3121 ttcggcaacg aggcgtgccc ggtcgtcgtc caccgctccc aggatgtagc cgccgccgtg
     3181 gagccacaga acccccgggc cgccctcggc aacgggagac cggtagatca ggagccgcag
     3241 gtcgccgcct ggcccggcga tgatctgctc ggtgacttcc acgtccgtct ccacgtcggg
     3301 agcgggagcg gccgccagca cagcgcgggt agcgtgcaga gtctcacgcg tgatcagatc
     3361 gagctccgcg ggcagaaaat ccagcgcagg ccggagttcg ggatcaatat tcgagtaatc
     3421 gagttccatg tgtttctcca gctcgcttag tgctcgcgac ggtcatgaac ccgcagatcc
     3481 cgactgccat atcttaccta cgctcagttg atcaaggcct caccgctcgc tcggcggcag
     3541 caccggccgc cgcgtcggga aatcggccct gcgcgcggcg gcagcgagca catccgcctt
     3601 ggaccggtgt ccggccagtc cttccagctg cagcttggtc acgcccatca ggccgaactc
     3661 ctccacggcg tggcgcgcca gcgcttcgcc cggcgagatc cagatactat ccacggtttc
     3721 cttgtcgtcg tgccgaccgg tctgccccgg cggcgcctcg gcgatgaaga agcgcgtatc
     3781 gaaacgtcgc ggccggccaa gcggcgtcac gaaccggctg tggaaatgaa tgcggtccac
     3841 tgccagccgc aggccttcct gccggcagat ggtttccagg ctgacctgac cggcatgcag
     3901 cgggccccga tagctctgga agcgctcttc aaccgcatcg tcgtcgtagg ccagcagctc
     3961 gcccgcccgg tcgtacgcca ggagcaggcc ggcctcctcg aagctctcgc ggataccggc
     4021 gatccagtac ccgcgccatt ccgcacccag cgcccgctgc tgcgcctgct gcacgtcgct
     4081 gggccccacg cgatgctcgt cgtagcggtg caggtgatcg tcgccgtcga cccgcccgcc
     4141 gggaaagacg aacatcccgc cggcaaaagc ggcaccgctg gtgcgcttga gcatcaggat
     4201 ctcgaacccg tccgccgcat cgcgcacgag gatcaccgtt gccgccggac gtattggctg
     4261 cacctgactc atcaggcctc ccgatcgaaa cgcggcaggc tatccggttt ttacccggac
     4321 cgccacaggc ggtatctttg cccgcctgac catccgggga tttcacatga agctgagcgt
     4381 cgagtttcca agcgtcgcct atcgggaagg cccggagggc atcatccggc tggcgaaagc
     4441 catcgaggac atcggctacg accagctgga catgttcgac cacgtggtga tgggctaccc
     4501 caccgaaacg cgcgcagcac ccatctaccc accgcagatg ccgatcatgg aggctctcat
     4561 ggtgctcgcc tacgcggcgg ccgtgaccac caggatcggg ctcggcaccg aggtgctcgt
     4621 gctgccgcag cgccagcccg tgctggtggc caagcagatc agcaccctgg acacgctctc
     4681 cggcggccgg gtgcggctgg gtgtgggcgt gggctggcag gcttccgagt acgacgccct
     4741 cgaggagagc ttcgccaatc gcggccggcg catggacgag gccatccgcc ttctgcgcgc
     4801 ctactggtcc gaggaaaagg tggagttcgc cggcgagtac tacacctccc acgccatggc
     4861 catggagccc aagccgcccc agggcggcca gctgccgatc tggatcggcg ggctggcacc
     4921 tgcggcgctg cgcagggtgg gagaactcgg agacggctgg ctggccaccg cgttcacgga
     4981 cccggatcgc gccagagccg ccgtggcgac catccatcgc cacgccgagg acgccggacg
     5041 tgaccccgcc gccatcggcc ttcagcagat gctcgacgtg cccccccgcg acgactcggg
     5101 caagggcttt tacgcagacc tggatcaggt ggaggccaga gccctggccg tcaaggagat
     5161 ggggttcgag tggggggcgc tcaacgccac cgccatcttt cagtccggcg cccgcagcgt
     5221 ggacgcgatg atcgacaccc tcggcgcgct gcacgagcgg ctgcgcgccg cgaccgcctg
     5281 attattgccc gcgcaggcct tacaatggcc ccccgggagg ggtaaccgat gtcatctcaa
     5341 cgcgacgacc atgcctactg gcgggcgaac ctgaaactcg gggcggtgct gctcgggatc
     5401 tggttcgtcg tctccttcgg cttcggtatt cttctcgtcg agccgttgaa ccgcatcccc
     5461 ttgtttggct tcaagctcgg attctggtgg gcccagcagg gcgcgatctt cgtcttcgtc
     5521 atcctcatct tcgtctacag ccggctcatg aagcgcatcg atcggcgcta cggcgtccac
     5581 gacgacgaag actgacggca gcagcggcca gtggacgtcc agctactcac ctatctcttc
     5641 gtaggcctga gcttcgcgct ctacatcggc atcgccatct ggtcccgagc cgcatcgacc
     5701 cgtgagttct acgttgcggg cgggcacgtc catccagtgg ccaacggcat ggctaccgcc
     5761 gccgactgga tgagcgcggc ctcgttcatc tccatggccg gcatcatcgc cttcatgggg
     5821 tacgacggaa ccgtctacct gctcggctgg acgggcgggt acgtgctgct cgcgctgctt
     5881 ctggcgccct acctgcgcaa gttcggcgag ttcacggtcc cggacttcat cggcacgcgt
     5941 tactactcca acacggcccg cgtcgtcgcc gtggtgagcc tcatcgtcat ctccttcacc
     6001 tacgtcgcag ggcagatgcg cggcgtgggc atcgtcttct cccacttcct cggggtggag
     6061 atcgagctgg gcgtcgtcat cggcatggcc gtggtgttca tgtacgccgt gctggggggc
     6121 atgaagggca tcacctacac ccaggttgcc cagtactgcg tgctgatctt cgcctacctg
     6181 gttccggcga tcttcatatc catgctcatc accggcattc ccatccctca gatcggcctg
     6241 ggcgccgcgg tgaacgacgg ttccggggtc agcgtgctgg ccaggctgga cgcgacgctg
     6301 gtcgagctcg gcttcggccc gtacacccag ggaagcaaga gcaccatcga cgtcttcgcc
     6361 atcaccatgg ccctcatgat cggcacagcc ggcctgcccc acgtcatcgt ccgtttcttc
     6421 accgtgccca aggtctccga cgcccgcaaa tcggccggct acgcgctgct gttcatcgcc
     6481 ctgctctaca ccaccgcgcc ggcggtgggc gcgttcgccc ggctgaactt cgtggaaacc
     6541 gtgcacggca cggggtacgc cgacgtggcc ggctggttca agagctggga ggacgtcggg
     6601 ctcatcggct ggctggacaa gaacgaggac ggcgccatcc agtacgcgcc cggcgccccg
     6661 ttcgagggca aacccgaatt cgatggggtg cgcggcccgg acggccagcg gctgatccgc
     6721 aacgcgccga ccggcaacgc caacgaggtc tacgtggacc gcgacatcat ggtcctggcc
     6781 aatccggaga tcgccgcgct gccgccctgg gtcatcgccc tggtggccgc cggcggcctt
     6841 gccgccgcgc tctccaccgc cgccgggctg ctgctggtga tctcgacgag cgtctcccac
     6901 gacctgatca agaagaccat ggcgcggggc atcagcgacc ggcaggagct actttacgcc
     6961 cgcatcgccg cggcgctggc catcgccgtc gcaggctatc tgggcatcaa cccgcccggc
     7021 ttcgtcgccc aggtggttgc cttcgccttc ggtctggccg ccgcttccct gttcccggcg
     7081 atcctgctcg gcatcttcag caaacggatg aaccgggaag gcgccattgc ggggatggtg
     7141 gtgggcctga cgttcacgtt cgcctacatc gtctacttca agttcgtcgc gcccggcgac
     7201 aacaacgccg cccactggtg gttgggcatc tctcccgagg gcatcggcat ggtgggggcg
     7261 ctcgccaact tcgcgaccgc ggccatcgtc agccggttcg cgagcccggc gccggaggac
     7321 gtccagcaac tggtggaaaa aatccgcatc ccgcggggcg tggagacgcc ccacacccac
     7381 tgatcggccg gcccggtacc gggccagcga gagggctagc tgccgaagcg ggccttggcc
     7441 tcgcgtcggc gagcgtgcag aaccggctcg gtgtagccgt tgggctgctc caggcccttg
     7501 aagacgagat cgcaggccgc ctggaacgcc acgctggcat cgaagtccgg cgccatgttg
     7561 cggtaggcgg catcgcccgc gttctgcccg tccaccacgg cggccatccg ctgcatggtc
     7621 ccaagcacct gctcgcggct gcagatgccg tgatgcagcc agttggcgat atgctgagac
     7681 gagatgcgca gcgttgcccg atcctccatg agaccgacgt cgttgatgtc agggaccttg
     7741 gagcaaccca cgccatgatc gatccatcga accacatagc cgagaatgcc ctgggcgttg
     7801 ttgtcgagct cggcctgcac ttcctctgcc gacaggttgc gtccgccgag gagcgggatg
     7861 cggagaatgt catccaggct cgccggctcc cgggcggcca gcgcctgctg acgctcgaaa
     7921 acgctcacct gatgatagtg ggtggcgtgc agggtagccg ccgtcggcga gggcacccag
     7981 gccgtgttgg caccggcctg ggggtgagca atcttggctg ccagcatctc cttcatctcg
     8041 tccggcttgg gccacatgcc cttgccgatc tgggcgaccc cgctgaaacc ggctgccagc
     8101 ccggcgtcca cgttggcgtc ctcgtaagcc tgaatccacg gctccgcctt gatggcttcc
     8161 ttgggcagca ccggaccggc ctgcatgctg gtgtgaatct cgtcccccgt gcggtccagg
     8221 aaaccggtgt tgatgaacac cacgcgctcg cgggcttcga atatgcaggc acggagattg
     8281 accgtggtgc ggcgctcctc gtccatgatg ccgaccttga gcgtgttcgg cccaaggccg
     8341 agcagctgct ccacccggga gaacagatcg caggtgaacg ccacctcttc gggtccgtgc
     8401 atcttgggct tgacgatgta aaccgacccg gctcgggagt tcctcaccga gccggtccca
     8461 agaaggtcgt gcatcgcggc aaaggcggtc accgccgcgt cgaggatgcc ctcgggaatc
     8521 tcgttgccgg caccgtcgag gacggcatcg gtggtcatga gatgccccac gttgcgcacc
     8581 agcatcaggc tgcgcccgtg cagggtcagc ggctggccgt ctagcccgac gtaggtccgg
     8641 tcaggattga gccggcgcgt gacggcgcgc ccacccttgt ccatcacttc ctcgagatcg
     8701 cccctcatca gcccgagcca gttcccgtag accacgcact tgtcctcggc gtccacggcg
     8761 gccacagagt cctcgcagtc ctgaatggtg gtcacggcgg actcgagcac cacgtccttc
     8821 acgccggcgg ggtgcgcggc gcccaccggg tggctccggt cgatctggat ctcgatgtgc
     8881 aggccgttgt ggacgagcag cagagtgctg gggtcgtcct cgtcgatgaa gccgacgaac
     8941 tgttccgggt tctcgagacc cgtgttgccg ccgttgtcga gaatggcccg tagccgccga
     9001 ccgccggggc cgtcgccgat gccgtaggca atcacctcgc tgtggctggc accggcgagg
     9061 ggcaccgtct cgtccaggaa ccgggccgca cgctccacca cgagctcgcc ccgggcgggg
     9121 ttgtagctgg tgcccttctc ccgccccggt tcttcgggca gaacgtcggt tccgtaaagg
     9181 gcatcgtaga ggctgcccca gcgcgcgttg gcggcattga gggcgtagcg cgcgttcatc
     9241 acgggaacga cgagctgagg gccggcgatg cgcgcgatct cgtcgtcgac gtcggcggtc
     9301 aggatcttga aaggcgcggg ctcggggacg agatagccga tctgctccag aaaggcccga
     9361 tactcctgcg cgtccagggg acggcctcgg cggtcttcgt gccattcatc gatggtggcc
     9421 tggagctcgt cccgccgggc gagcagcgcc cggttgcggg gagcgaggtc gtcgacgatg
     9481 gactcgaagc ctgcccagaa cgcctccgca tccacgccgg tgcccggcgc gatccgttga
     9541 gaaaccagat catcaagctc ggcggccacc gagagtttgc cgcgggacac ccgcttcgtt
     9601 gcgctcatcg tcgccccctt ttggcc
//
LOCUS       DBNHOX010000126         2692 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1488502, whole genome shotgun sequence.
ACCESSION   DBNHOX010000126 DBNHOX010000000
VERSION     DBNHOX010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1488502"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1745
                     /gene="istA"
                     /locus_tag="AC16UZ_02745"
     CDS             180..1745
                     /gene="istA"
                     /locus_tag="AC16UZ_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HIR6245628.1"
                     /translation="MLVVETIAKIRRAHFIDGKSIKQICRELRVSRNTVRKVIRSGAT
                     EFSYDRTTQPRPRIDPWRSELDEMLAENARRPKRERLTLVRVYEELRNRGYGGGYDAV
                     WRYAASWSKAAQAESAGAYVPLIFDPGEAYQFDWSHEIVILDGVTVTVKVAHVRLCHS
                     RMPFVRAYPRETQEMVFDAHDRAFAFFGGACARGIYDNMKTAVDAIFVGKDRAYNRRF
                     QQMCGHYLVDPVACTPASGWEKGQVENQVGVLRRRFFVPRPKFKSYAELNAWLEDRCI
                     AYAKANRHPDIPDKTVWEMFEEERPSLIPYVGPFDGFHAIPASVSKTCLVRFDKNRYS
                     VDARAVGRPVEIRAYADRLECWQGGKAVAGHVRVFGRGKTIYDPLHYLPVLARKPGAL
                     RNGAPFKGWELPSALRRVQRKLERQPGGDRQMVEILGAVLTDGLEAVEAACSEALSHN
                     VHSAGVVLNILARHREPPPPLTITTPDALRLTCEPAADCDRYDSLRRTRNGTIASAGR
                     HGAAEALRHEDSL"
     gene            1678..2406
                     /gene="istB"
                     /locus_tag="AC16UZ_02750"
     CDS             1678..2406
                     /gene="istB"
                     /locus_tag="AC16UZ_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043766072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HIR6245629.1"
                     /translation="MERSQVLDAMGQLKLYGMRTAYDEVIATAVKRQHEPQQIIGDLL
                     SAEISEKQARSIKYQMTIAKLPLAKELGEFDFKATEVNETLIRDLATGDFLDHQRNLV
                     LIGGTGTGKTHLAVSIARACIRNGRRGRFFNVVDLVNKLDAEARAEWQGRTAELISRL
                     DFLILDELGYLPFAQTGGQLLFHLISKLYERTSVIVTTNLAFGEWPTVFGDAKMTTAL
                     LDRLTHHCEIVETGNESWRFKNRA"
BASE COUNT          527 a          846 c          871 g          448 t
ORIGIN      
        1 cgaaaggatt agcaatgacg acgacaagca tcgaccctgc tgacatcatc cgtctgaagg
       61 ttccctgtgg atcggcatgc aagattgacc cctgtattgg ggtgatcggc gtccaaaagt
      121 gacccccctg atacttttga tgagaagctt cctcggagcc atgagggagc gttcagggga
      181 tgttggttgt ggagacgatt gcgaagatca ggcgtgcgca tttcatcgac ggcaagtcga
      241 tcaagcagat ttgccgggag ctgcgggtat cgcggaatac ggtgcgcaag gtgatccgct
      301 cgggcgcgac cgagttcagc tatgatcgga cgacgcaacc tcgccccagg atcgatccgt
      361 ggcgatcaga actggatgag atgctggccg agaatgcccg gaggccgaag cgggagcggc
      421 tgacgctggt ccgggtctac gaggagctgc gcaaccgtgg ctatggtggc ggttacgacg
      481 ccgtgtggcg ttacgcggcc agttggtcga aggcggcgca ggcagagtct gccggcgcct
      541 atgttccgct catcttcgat ccgggcgagg cctaccagtt cgactggagc catgagatcg
      601 tgatcctcga tggcgtgacg gtgacggtca aggtcgccca tgtccgtctc tgccacagcc
      661 ggatgccgtt cgtgcgggcc tatccgcgcg agacgcagga gatggtgttc gacgcgcacg
      721 acagagcatt cgccttcttc ggcggcgcct gtgcacgggg catctacgac aacatgaaga
      781 cggcggtcga tgcgatcttc gtcggcaagg atcgcgccta caatcgccgg ttccagcaga
      841 tgtgcgggca ttacctggtc gatcccgtcg cctgcacccc ggcctcgggc tgggagaagg
      901 ggcaggtcga gaaccaggtg ggcgtgctgc ggcggcggtt cttcgtgccc cgcccgaagt
      961 tcaaatccta cgccgagttg aatgcctggc tcgaggatcg gtgcatcgcc tatgccaagg
     1021 ccaaccggca ccccgacatc ccggacaaga cggtctggga gatgtttgag gaagagcggc
     1081 ccagcctgat cccctatgtc ggcccgttcg atggcttcca cgcgatcccc gcgtcggtgt
     1141 ccaagacctg cctcgtgcgc ttcgacaaga accgatattc ggtggatgcc cgcgccgtgg
     1201 gacggccggt cgagatcagg gcctacgctg atcgcctgga atgttggcag ggcggcaagg
     1261 ccgtcgccgg acatgtgcgc gtatttggcc gcggcaagac gatctacgac ccgctgcact
     1321 acctcccggt cctggcccgc aaacccggcg ccttgcgcaa tggcgcgccg ttcaagggct
     1381 gggaactgcc atctgccttg aggcgcgtgc agcgcaagct ggaacgccag cccggtggcg
     1441 accggcagat ggtggagatc ctcggcgccg tgctcaccga tgggctggag gcggttgaag
     1501 ccgcctgctc ggaggcgctg tcacacaacg tgcattccgc cggcgtggtc ctgaacatcc
     1561 tggcccgcca ccgcgagccg ccgccgcccc tgacaatcac caccccggac gcgctgcggc
     1621 tgacctgtga gcccgccgcc gattgcgacc ggtacgacag cctgaggaga acaaggaatg
     1681 gaacgatcgc aagtgctgga cgccatgggg cagctgaagc tctacggcat gaggacagcc
     1741 tatgacgagg tcatcgccac ggcggtcaag cgccagcacg aaccccagca gatcatcggc
     1801 gatctgctga gcgccgagat cagcgagaag caggcccgct cgatcaaata ccagatgacg
     1861 atcgccaagc tgccgttggc caaggaactc ggagagttcg acttcaaagc aacggaggtc
     1921 aacgagaccc tgatccgcga cctcgccacc ggagacttcc tcgaccatca gcgcaatctc
     1981 gtcctgatcg gcggcaccgg aaccggcaag acccatctcg ccgtcagcat cgcacgcgcc
     2041 tgcattcgca acggcaggcg cggcaggttc ttcaacgtcg tcgacctggt gaacaagctg
     2101 gacgctgaag cccgggccga gtggcaaggc cggaccgctg agctgatctc ccgcctcgac
     2161 ttcctgatcc tggacgagct tggctacctc ccgttcgccc agaccggcgg ccagctcctg
     2221 ttccacctca tcagcaagct ctacgaacgc acctcggtga tcgtgacgac caacctggcg
     2281 ttcggcgaat ggccaaccgt cttcggcgac gcaaagatga ccacagcgct cctcgatcgc
     2341 ctcacccacc attgcgagat tgtcgagacc ggcaacgaaa gctggcgctt caagaaccga
     2401 gcatagtgac cacggcaacc acgctggccc gccgcagctc cgttacatgg ggactacgca
     2461 gcgccgggcc agctcagctc cagggggggg caatattgcg cgccgatacg gggtcacgat
     2521 tggatgccga ttgacagtga aggacccgga gaccggcaag cgcgtctcgc gcccgaaccc
     2581 cgaggccgac tggatcatca aggaagtgcc ggagcttcgg atcatcgatc aggtgctctg
     2641 ggagcaggtg aaggcgcggc aggacgaaca ggccgccagc gagttcggtg ac
//
LOCUS       DBNHOX010000127         3061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1038592, whole genome shotgun sequence.
ACCESSION   DBNHOX010000127 DBNHOX010000000
VERSION     DBNHOX010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1038592"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="AC16UZ_02755"
     CDS             complement(<1..1574)
                     /locus_tag="AC16UZ_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012361.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR6245630.1"
                     /translation="MALQCAAVFDRMTPMADPAQSSSSFSFVDMEHQILDFWEQGRVF
                     QRSLENTREKKPYIFYDGPPFATGLPHHGHIVASTIKDIVPRYWTMQGRYVLRRFGWD
                     CHGLPIEHEIDKQLNMSAQQAVAEMGVAGYNQQCRAIVQRYVDEWRHTITRLGRWVDF
                     DNDYKTMDAWYMESVWWVVKQLWEQGLVYQGVKVMPVSTALGTPLSNFEANQNYMDVQ
                     DPAITVLFKLVDEDAYLSAWTTTPWTLPSNLAICVGPDIDYVRVRDPQLGADVYMAAQ
                     RVADYGSFEEVGRVKGRELVGRRYEPLFPYFREAGEEGAFVVVMDDYVTTESGTGLVH
                     QAPAFGEDDYRIARAAGIDALVCPVTVNGEFTDEVTDFAGQHVKDADRDIIRYLKDHN
                     LLYRQDVIQHSYPYCYRSDTPLIYRAIPSWYVRVTEFVDKLVKNNQQIRWVPDHIKTG
                     RFGNWLEGAIDWSISRNRVWGTPIPIWINDVTGNEVCIGSIDELEALTGVRVDDLHRE
                     HVDPLTFTLQGEEGTY"
     gene            1636..2499
                     /locus_tag="AC16UZ_02760"
     CDS             1636..2499
                     /locus_tag="AC16UZ_02760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6245631.1"
                     /translation="MEFGAVFPHNEIGTDPGAIKAYAQGVEAMGATHLLIYDHVLGAD
                     PNRPGGWKGAYDKDVAFHEPFTTFAFIAAVTDKLEMITTVLILPQRQTVLVAKQAAEV
                     AILSNNRFRLGVGSGWNQVEYVGLNEDFHTRGRRQAEQVELMRKLWSEDSLTFDGAFH
                     TIDAASINPRPGASVPVWFGGSAAPLLKRCAELGDGWIPLMGANPKARECIDTIRAHR
                     EAAGLPWEGFGIQAQAQYAGGNPDRWASHAGKWQDMGCTHLAIATHNAGPTNADGHLR
                     RIEEYLQAVAA"
     gene            complement(2524..>3061)
                     /locus_tag="AC16UZ_02765"
     CDS             complement(2524..>3061)
                     /locus_tag="AC16UZ_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6245632.1"
                     /translation="GWGEFHVVGHSMTGMATQRLALEAPERVRSAVAVCPVSAAGMAM
                     DDQTRAFFESTTTDDEAFRGLIGMISGSLSGGWVDAKLRWNREAAAPDVRLDYLAMFA
                     DTDFAESVEGLETPFLVMVGEHDAEGLDAAAMQKTYLAWHPNAELVVIPNCGHYPMQE
                     CPPFFVSTLESFLRRRAG"
BASE COUNT          562 a          992 c          976 g          531 t
ORIGIN      
        1 tggtaggtgc cttcctcgcc ttggagcgta aaggtcagcg gatccacgtg ctcgcggtgc
       61 agatcatcga cgcggacgcc ggtgagcgct tccagctcgt cgatggagcc gatgcagacc
      121 tcattgcccg tgacgtcgtt gatccagatg ggaatcggcg tgccccagac ccggttgcgg
      181 gagatggacc agtcgatggc gccttccagc cagtttccga accgcccggt cttgatgtgg
      241 tcgggaaccc agcggatctg ctggttgttt ttgacaagct tgtcaacgaa ctccgttacc
      301 cgcacgtacc aggacggtat ggcccgatag atcaggggcg tatcgctgcg atagcagtag
      361 gggtagctgt gctggatcac gtcctggcgg tagagcaggt tgtgatcctt cagatagcgg
      421 atgatgtcgc gatccgcgtc cttgacatgc tgcccggcaa agtcggtgac ctcgtcggtg
      481 aactcgccgt tgacggttac cggacagacc agggcatcga taccggcggc ccgcgcgata
      541 cggtagtcgt cctcgccgaa ggccggcgcc tggtgcacca ggccggtgcc gctttcggtg
      601 gtgacgtagt cgtccatcac caccacgaac gcgccctcct cgcccgcctc gcggaaatag
      661 gggaagagcg gctcgtagcg gcgacccacg agctcgcgcc ctttgacgcg ccccacctcc
      721 tcgaagctgc cgtagtcggc tacgcgctgc gccgccatgt agacgtcggc gcccagctgc
      781 gggtcccgaa cccggacgta gtcgatatcg ggacccacgc agatggccag gttggacggc
      841 agcgtccacg gcgtggtcgt ccacgccgac aggtaggcgt cctcgtctac gagcttgaac
      901 agcaccgtga tggccggatc ctgcacgtcc atgtagttct ggttggcctc gaagttggac
      961 agcggtgtcc caagggccgt tgaaaccggc atcaccttga cgccctggta gacgagcccc
     1021 tgctcccaga gctgctttac gacccaccag accgactcca tgtaccaggc gtccatggtc
     1081 ttgtagtcgt tgtcgaaatc gacccagcgg ccgaggcgcg tgatcgtgtg gcgccactcg
     1141 tcaacgtagc gctgcacgat ggcgcggcac tgctggttgt agccggcgac ccccatctcc
     1201 gccaccgctt gctgggcaga catgttgagc tgcttgtcga tctcgtgctc gattggcaaa
     1261 ccgtggcaat cccagccgaa gcgacgcagt acgtagcggc cttgcatcgt ccaatagcgc
     1321 ggcacgatgt ccttgatggt gctggccacg atgtgaccgt ggtgaggaag accggtcgca
     1381 aagggcggac cgtcgtagaa gatgtagggt ttcttctccc gcgtgttctc caggctgcgc
     1441 tggaacacac ggccctgctc ccagaaatcc agaatctggt gctccatgtc cacgaacgag
     1501 aacgatgagc tcgattgtgc tgggtcggcc attggggtca tgcggtcgaa aacggccgcg
     1561 cattgtaacg ccattgcgcg ccattccgta gggcactaag ctcgcccgtt cgccgcagga
     1621 ctcgagggga tgtcaatgga attcggagcc gtatttccgc acaacgagat cggaacggac
     1681 cccggcgcca tcaaggccta cgcccaggga gtggaagcca tgggcgcgac ccatctgctg
     1741 atctacgacc acgtgctggg cgcggatcca aaccgtccag gcggctggaa gggggcgtac
     1801 gacaaggacg tggctttcca cgaacccttc accacgttcg ccttcatcgc cgcggtaacc
     1861 gacaagctcg agatgatcac tacggtgctg atcttgcccc agcggcagac ggtgctggtc
     1921 gcaaagcagg cggccgaggt ggccattctc tccaacaacc ggttccgcct gggcgtgggt
     1981 agcggctgga atcaggtgga gtacgtgggg cttaacgaag acttccatac ccggggcagg
     2041 cgccaggcgg agcaggtcga gctcatgcgc aagctctgga gcgaggacag cctgaccttc
     2101 gacggcgcct ttcacaccat cgacgccgcc agcatcaatc cgcgccccgg cgccagcgtc
     2161 ccggtgtggt tcggcggcag cgccgcgccg ctgttgaagc gctgcgccga gctcggcgac
     2221 ggctggatac ccctcatggg cgcgaatccg aaggccaggg aatgcatcga cacgatccgt
     2281 gctcaccgcg aagccgccgg gctgccctgg gagggattcg gtattcaggc ccaggcccag
     2341 tatgcgggcg gcaaccccga tcgatgggca tcccatgcag gcaagtggca ggacatgggc
     2401 tgcacccacc tggccatcgc cacccacaac gcggggccga cgaacgcgga cggtcatctg
     2461 cgccgcatcg aggaatacct tcaggccgtg gcagcctgac gccgccggga gctggcagga
     2521 cgatcagccc gcccgtctgc gcaggaaaga ctccagagtg ctgacaaaga acggcggaca
     2581 ctcctgcatg gggtagtgac cgcagttcgg aatcaccacc agctccgcat tggggtgcca
     2641 ggcaaggtag gttttctgca tggcggctgc gtcgagacct tccgcgtcgt gctctccgac
     2701 catcaccaga aacggcgttt cgagcccctc caccgactcc gcaaagtcgg tatcggcaaa
     2761 catggcgaga tagtcgagcc gcacgtccgg ggccgccgcc tcgcggttcc agcgcagctt
     2821 cgcgtccacc caaccgcccg aaagcgagcc gctgatcatg ccgatcagcc cgcgaaaggc
     2881 ttcatcgtcg gtggtggtgc tctcgaagaa cgcccgcgtc tgatcatcca tggccatgcc
     2941 ggctgcggac acagggcaga cggccaccgc gctcctgacg cgctccgggg cctccagggc
     3001 aagccgctgg gtcgccatgc ccgtcatgga gtgaccgacc acgtgaaact caccccagcc
     3061 c
//
LOCUS       DBNHOX010000128         4361 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_845498, whole genome shotgun sequence.
ACCESSION   DBNHOX010000128 DBNHOX010000000
VERSION     DBNHOX010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_845498"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="AC16UZ_02770"
     CDS             complement(<1..436)
                     /locus_tag="AC16UZ_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR6245633.1"
                     /translation="MNSSASDRVIWRNGEFVPWADATVHVLAQSLQRGTLAFDFMSVH
                     ETADGPAIFRLEAHIERLLQSCRLEELPILYPAEALMEACAETVRRNPGCKSVKISAL
                     IASIEVELIPQDPAVAVFIAAYDSARDIMAKHQTPYPTRETLA"
     gene            complement(462..1457)
                     /gene="dgcA"
                     /locus_tag="AC16UZ_02775"
     CDS             complement(462..1457)
                     /gene="dgcA"
                     /locus_tag="AC16UZ_02775"
                     /inference="COORDINATES: protein motif:HMM:NF042940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-D-Glu racemase DgcA"
                     /protein_id="HIR6245634.1"
                     /translation="MDVFAHIESWPIAGTFTIARGSKTVAEVVVVTVREGDLTGMGEC
                     LPYPRYGQSPEGTLEEIRVATAGHAILDRQRLTELMPAGPARNALDCALWDLEARKSG
                     QPVWRLAGLAEPRPVVTALTIVLGEPGQMREAAERHSRRSLLKIKLGAGAGRDIERLR
                     AVRSGAPDARLIVDANEGWTLGELEQVAPVAADLGVEMIEQPLPVSEDEALDAFRCPV
                     PLAADESLRAGEDLDRLAGRYQVANIKLDKTGGLTEALILQKKAETLGMDVMVGCMVA
                     TSLSMAPALLLAQTARFVDLDGPLLLVRDRQPGLDYHDSLVAFPQQAVWGAGEHG"
     gene            complement(1474..2517)
                     /gene="dgcN"
                     /locus_tag="AC16UZ_02780"
     CDS             complement(1474..2517)
                     /gene="dgcN"
                     /locus_tag="AC16UZ_02780"
                     /inference="COORDINATES: protein motif:HMM:NF041892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase DgcN"
                     /protein_id="HIR6245635.1"
                     /translation="MRSPNDMASAGTRIEAPYLIFLGSAHDQLAAKTGQGIVDWRPQN
                     CIGQLRLPECQADLGLPDLSVAEAAAAGARTLVIGTVSPGGSLPEKWLPILHAALANG
                     LDIASGMHDRLDSFPEIRDAAARHDRRLVDVRHSTVRFPTGSGRKRSGRRLLTVGTDC
                     SVGKKYAALALEREMRSRGMDAEFRATGQTGILIAGSGVAIDAVTADFISGAAECLTP
                     DAERGHWDVVEGQGSIFHPSFAGVSLGLLHGTQPDAVVVCHEPTRRTMRNVSTLMPRI
                     GEVIEQTLVHGRTTNPDVVCIGVAVNTAALDEGEARELLTNYAAQLGLPVTDPIRFGA
                     GLLVDELVKNFPD"
     gene            complement(2553..3569)
                     /locus_tag="AC16UZ_02785"
     CDS             complement(2553..3569)
                     /locus_tag="AC16UZ_02785"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6245636.1"
                     /translation="MDTAEPGSQAWLDLVEEPIIDPDRVIVDPHHHLWDQPSAFGRYT
                     LEDLWRDTGSGHRIAKTVFLECGASYRESGPEHLRCVGETEYVTQAARASSAQSDGNA
                     VIAGIVARADLRLGDALDEVLDAHVEVAGDLFKGIRHSGAHAREPEILRIAGRAPAQL
                     YANDAFRRGVRRLGELGLTYDTWHYHYQNPQFLALARAVPDTIMVLDHFGTPLGVGPY
                     ADRREEIFRQWQDDMSRLADCPNVVAKLGGLAMPDNGFGWHAAARPPTSEEFAEAQRP
                     YYRHMIDVFGADRCMFESNFPVDRMSLSYAVLWNGMKRIAADFSEAEQDALFSGTATR
                     VYRL"
     gene            complement(3701..>4361)
                     /locus_tag="AC16UZ_02790"
     CDS             complement(3701..>4361)
                     /locus_tag="AC16UZ_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6245637.1"
                     /translation="ISSKVLPKLRAKYPSLKVGVSGEQEERTKAFTGLAQAAMLALIV
                     IYALLAIPLRSYLQPLVIMSVIPFGAVGAIVGHWLMGYQLMFFSALGMVALSGVVVNA
                     SLVLVDYTNRRRREGMPIAEALRSAGVVRFRPILLTSVTTFVGLIPLMFSDTPATAFF
                     IPMAISLAFGVLFATAITLLLVPALYHLAEDVSGWDPVAQGVEEHRSNIRSTGKLQPG
                     G"
BASE COUNT          733 a         1424 c         1456 g          748 t
ORIGIN      
        1 gggccagggt ctcccgtgtc ggatacgggg tctggtgctt ggccatgatg tctctcgcgc
       61 tgtcgtacgc cgctatgaac acggccacag cgggatcctg aggtatgagc tcgacctcga
      121 tggaggcgat caacgcgctg atcttcacgg atttgcagcc ggggtttcga cgcaccgtct
      181 ccgcgcaggc ttccatcagc gcctcggccg gatacaggat gggcaattcc tccaggcggc
      241 aactttgcag cagacgctcg atgtgggctt ccaggcggaa gatcgcgggc ccgtccgcgg
      301 tttcatgaac gctcatgaag tcgaaggcca gggtgccgcg ctgcaggctc tgggcgagca
      361 cgtgcacggt agcgtctgcc cagggcacga actcgccgtt gcgccagatc acccgatcgg
      421 atgctgagga attcatgtgg tcgtcgtttc cttaagcttc tctacccgtg ttcgccggca
      481 ccccaaacgg cctgctgggg gaaagccacc aggctgtcgt ggtagtcgag acccggctgc
      541 cggtcgcgca cgagcagcag cggtccgtcc aggtccacga agcgcgcagt ctgcgccaaa
      601 agcagggccg gtgccatgga gagcgacgtc gccaccatgc agcccaccat cacgtccatg
      661 ccgagcgttt ccgccttctt ctgcaggatc agagcttcgg tgagcccgcc ggtcttgtcg
      721 agcttgatgt tcgcgacctg gtagcggcca gcgagccggt cgagatcctc accggcgcgc
      781 agactttcgt cggctgccag cgggacgggg cagcgaaaag cgtccagagc ctcatcctcg
      841 gatactggca aaggctgctc gatcatctcc acgcccagat ctgccgccac cggagcgacc
      901 tgctcgagct ctcccagcgt ccagccttcg ttggcatcga cgatcaggcg ggcgtccggg
      961 gcgccgctgc gcaccgcgcg caggcgctcg atgtcccgcc cggcacccgc gccgagcttg
     1021 attttcaaca gcgaacggcg agagtgccgt tcagccgctt cacgcatctg cccgggctcg
     1081 ccgagcacga tggtcagcgc cgtgaccacg ggccgcggct ctgcaagccc tgcgagcctc
     1141 cagaccggct gtccggattt ccgcgcctcc agatcccaca gcgcgcagtc cagcgcgttc
     1201 ctcgctgggc cggcaggcat cagctccgtc aaccgttgac gatccaggat ggcgtggccg
     1261 gctgtggcga cccggatctc ttccagggtt ccctccggag actgtccgta gcggggatag
     1321 ggcagacatt cgcccatgcc ggtcaggtcg ccctcacgca cggtcaccac gaccacctcg
     1381 gccaccgtct tgctgcctcg ggcgatggta aaagtgccgg cgatgggcca actctcgatg
     1441 tgtgcgaaaa cgtccatggg gagccggcgg ctttcagtcc ggaaagtttt tgacaagctc
     1501 atccacgagc aagcccgcgc cgaagcggat gggatccgtg acgggcagcc cgagctgcgc
     1561 ggcgtagttg gtcagcagct cacgagcctc gccctcgtcg agtgcagccg tgttcaccgc
     1621 gacgccgatg cagacgacgt cggggttggt ggttcgacca tgcacgagcg tctgctcgat
     1681 cacctcgccg atgcgcggca tgagcgtgct gacgtttcgc atggtccggc gggtcggctc
     1741 gtggcagacc acgacggcgt ccggctgggt cccgtgcagc agcccgaggg aaacgccggc
     1801 gaaagaaggg tgaaagatcg agccctgacc ctcgaccacg tcccagtggc cgcgttccgc
     1861 atccggggtc aggcactccg cagcgcccga gatgaagtcc gccgtgacgg cgtcgatcgc
     1921 gacgccgctg ccggcgatga gaatgcccgt ctggcccgtg gccctgaact cggcgtccat
     1981 gcccctgctg cgcatctcgc gctccagggc gagcgccgcg tacttcttgc ccactgagca
     2041 gtcggtgccg acagtgagca accgtcggcc gctacgtttt ctgcccgacc ccgtagggaa
     2101 acggacggtg ctgtgacgca catcgaccag acgacgatcg tggcgcgccg cagcatcccg
     2161 gatctccgga aagctgtcca accggtcgtg cattccgctc gcgatgtcga gaccgtttgc
     2221 cagcgcggcg tgcaggatgg gcagccattt ctcgggcagc gaaccgcccg ggctgaccgt
     2281 tccgatcacg agggtgcggg cgccggctgc cgccgcttcg gccacggaca aatcgggtag
     2341 gccgagatcc gcctggcact cgggcaatct cagctgcccg atgcagttct gcggccgcca
     2401 gtcgacgatg ccctggcccg ttttcgcggc cagctggtcg tgggcgctgc ccaggaagat
     2461 caggtaaggc gcttcgatcc tcgtgccggc tgacgccatg tcgttcggac tgcgcaatct
     2521 gggaacctcc gccaggtcgc cgtcgtctct gatcaaaggc ggtacacgcg ggtcgcggtg
     2581 ccgctgaaca acgcatcctg ctccgcttcg ctgaaatcgg cggcgatgcg cttcatgccg
     2641 ttccacagca ccgcgtatga cagcgacatg cgatccaccg gaaaattgct ctcgaacatg
     2701 caccggtcgg cgccgaacac gtcgatcatg tgccggtagt aggggcgctg ggcctcggcg
     2761 aattcctcgg aggtcggcgg ccgggcagcg gcgtgccaac cgaaaccgtt gtccggcatg
     2821 gccagcccgc ccagcttggc gaccacgttg gggcagtcgg ctagccggga catgtcatcc
     2881 tgccactggc gaaagatctc ttcgcgccgg tccgcgtaag gaccgacgcc cagcggggtg
     2941 ccgaaatggt cgagcaccat gatggtgtcc ggtacggccc gtgccagcgc cagaaactgg
     3001 gggttctgat agtgatagtg ccaggtgtcg taggtcaggc cgagctcgcc gagccgtctg
     3061 acgccgcggc ggaaggcatc gtttgcatac agctgcgcgg gggcccggcc ggcgattcgg
     3121 aggatctcgg gctcccgggc gtgggcccct gagtggcgga tgcctttgaa caggtctccc
     3181 gcgacctcga cgtgggcgtc cagcacctcg tccagggcgt cgccaagccg cagatcggct
     3241 cgggccacga tgccggcaat caccgcattt ccgtcgcttt gagcagagga ggcgcgggcg
     3301 gcctgcgtga cgtattccgt ctcgccaacg caacgcaggt gctcggggcc cgattcccgg
     3361 taacttgctc cgcattccag aaacacggtt ttcgcgatgc ggtggcctga ccccgtgtcg
     3421 cgccacaggt cttccagcgt gtagcgtccg aacgcgcttg gctggtccca gaggtggtga
     3481 tggggatcga cgatcacgcg atcgggatcg atgatcggtt cctcaacgag gtccagccag
     3541 gcctggctgc caggttcggc ggtatccaag gcgtcgtctc cgagcggcca ttcaggagtc
     3601 gtcatcttag tacaggacgg ctcggtcatg ctcgcgtctg gatggggcgg tggggtttgg
     3661 cttaccccgg agcgctgccc gggtcgtcag ggcgctcgcg tcagccgccc ggctgcagtt
     3721 tgcccgtcga gcggatattt gagcgatgct cttcaacgcc ctgggcgacg gggtcccagc
     3781 cggagacgtc ctcggccagg tggtaaagcg cgggcactag cagcaacgtg atggcggtcg
     3841 cgaacagcac gccgaaagcc agcgagatgg ccatggggat gaagaatgcg gttgccgggg
     3901 tgtcgctgaa catcaacggg atgaggccga cgaacgtggt cacggaggtc agcaggatgg
     3961 gcctgaatcg gaccacgccg gcgctgcgca gggcttccgc gatcggcatg ccctcccgac
     4021 gacggcgatt cgtgtagtcc accagcacga ggctggcgtt cacgaccacc ccggacagag
     4081 ccaccatgcc cagcgccgag aagaacatca gctgatagcc catgagccag tgaccgacga
     4141 tggcaccaac ggctccgaag ggaatgacgc tcatgatgac cagcggctgc aggtaggatc
     4201 ggaggggaat tgccagcagc gcgtagatga cgatcagagc gagcatggcg gcctgggcca
     4261 gtcccgtgaa cgctttggtc ctctcttcct gctctccgga gacgccgacc ttcaaagagg
     4321 ggtacttggc gcgaagcttc ggcagcacct tggatgagat g
//
LOCUS       DBNHOX010000129         3485 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1296037, whole genome shotgun sequence.
ACCESSION   DBNHOX010000129 DBNHOX010000000
VERSION     DBNHOX010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1296037"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="AC16UZ_02795"
     CDS             <1..549
                     /locus_tag="AC16UZ_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6245638.1"
                     /translation="GRLEVGLGAGWNEEECDAYGIELGTLTERFDRFDEACAVIHGML
                     SVPTTDFAGRYFTLTAARCNPPPLQQPHPPICIGGNGERRTFPNVVRWAQHWNYPGFD
                     VDGFVAKRDVLWDQCRAVGRDPGEIMTSAHQPVDLDDLDDLRRRVTAFREAGLGLLIF
                     YLPPPHRALALEPIAEIAAQVG"
     gene            complement(546..1655)
                     /locus_tag="AC16UZ_02800"
     CDS             complement(546..1655)
                     /locus_tag="AC16UZ_02800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6245639.1"
                     /translation="MTSSPVNPLRSDEHPFKLAVFGINVSGGCTMTRAPGTLEVSWPE
                     TRQVALAAEQAGFDALIPVARWKGMGGAVNFNHRSWEPFTLASALAAITKRIALFSTC
                     HVPTVHPVRLAKESATVDQISGGRFCLNIVAGWNEREIGMFGVQQKPHDERYDVADDW
                     IHLTKRLWTETQEFDHQGPYFSAPGAYSEPKPVQAPHPLIMSAGNSPRGLAFAAEHAD
                     LNFVVAADLDKAGESASAVRAKAAEQGRSIKVFGQGYIVCRSSEAEARAARDRYVHEL
                     GDWEGVRNLLDVLIPNSQSALGDEWEAMAANMIAGYGALPLVGTPDQVVDGMQAFSDA
                     GLDGMTLSWLDYPAGIEQFEKELRPRLVEAGLRAR"
     gene            complement(1652..2641)
                     /locus_tag="AC16UZ_02805"
     CDS             complement(1652..2641)
                     /locus_tag="AC16UZ_02805"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6245640.1"
                     /translation="MSLASAQTIDVHAHAVLEETMGAAGEHGPELGREAAPRFRVGNY
                     ELHGVRYRGSPFMDPDLRLDAMDAAGIDYQVLSPNPLTYFHFIDGSAARDFCRIHNDT
                     LSAIVGERADRLGAFAALPMQDVDFAIEETERAVRELGMYGPYIGTDFGRPLNDPALD
                     PFYEKLIELDVPLFIHPAPAGIDGPPGDPNLKQFDLDIIIGFAAQETIATCTLIYGGV
                     LERHPKLDVCLSHGGGATGYAFGRMASAAKKRPWASDSLRQDGAFEALLQRLWFDVHV
                     HDPDALALLHKKVNPDHLVFGTNFAGWDQQPGDVRHDAAPYTENARRLLRATR"
     gene            2727..>3485
                     /locus_tag="AC16UZ_02810"
     CDS             2727..>3485
                     /locus_tag="AC16UZ_02810"
                     /inference="COORDINATES: protein motif:HMM:NF024375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6245641.1"
                     /translation="MTSSDWGRVRPADIPWKQFDDPHGRPTTPVRVLKGGDPAVLEVR
                     FPPGYVAGLHWHPYDTLYIVTRGSMQIGDEGVYRPGDIRWVRAGHVYGPEAAGAEGVD
                     FFLVSLGGEIELNWADLVDVPERLRERLETSGEPWGRVNLQEVPWQDFEDPAGRPTQP
                     VQVLLDDDPYVLRTRFSPDYVAGDHWHDFDTLYFITSGSMRFGDEGWYEPGEVRWVRG
                     GHSYGPEEPGEGGVEFILVSCGGPVNLHWSDLEPP"
BASE COUNT          571 a         1095 c         1184 g          635 t
ORIGIN      
        1 ggtcggctgg aagtcgggct cggggcagga tggaacgagg aggagtgcga cgcctacgga
       61 atcgagctgg gaaccctgac tgagcgcttc gaccgtttcg atgaggcctg tgcggtcatt
      121 cacggcatgc tgagcgttcc gacgacggat tttgcgggac gatacttcac gctgaccgcc
      181 gcccgctgca atccgccgcc gcttcagcag ccgcacccgc ccatctgcat tggtggcaac
      241 ggcgagcggc gtacgttccc caacgtcgtg cgctgggcgc agcactggaa ctacccgggg
      301 ttcgacgtcg acggatttgt tgcgaagcgg gacgtgctgt gggatcagtg tcgggccgta
      361 ggccgagacc caggcgagat catgacgtcc gcccatcagc cggtagatct ggacgatctg
      421 gacgacctgc gccggcgcgt cacggccttc cgcgaggcgg gcctgggatt gctgattttt
      481 tacctgccgc cgccgcaccg ggcgctagct ctcgagccca tcgccgagat cgctgcacag
      541 gtgggttagc gggcgcgcaa cccggcctcg acgagccggg gtcggagctc cttttcgaat
      601 tgctcgatgc cggctggata gtcgagccag ctgagcgtca tcccatcgag ccctgcgtcg
      661 ctgaaggcct gcatgccgtc aacgacctga tccggcgtgc ctaccagcgg aagcgcgccg
      721 tagccggcga tcatgttggc ggccatggcc tcccactcat cgcccagcgc gctttgagag
      781 ttggggatga ggacgtcgag caggttgcgg acgccttccc agtcgccgag ctcgtggacg
      841 tagcggtccc gggctgccct ggcttcggcc tcgctggaac ggcaaacgat gtaaccctgg
      901 ccgaatacct tgatggaacg cccctgctcg gctgccttcg cccggaccgc gctggcactt
      961 tcccctgcct tgtcgagatc ggccgcgacg acgaagttga ggtctgcgtg ctcggcggcg
     1021 aatgccagac ctctggggct gttcccggcg ctcatgatca gagggtgagg cgcctgcacg
     1081 ggcttgggct cggagtaggc gccaggtgct gagaaatagg gaccctgatg gtcgaactcc
     1141 tgagtttccg tccacaatcg tttggtcaga tgaatccagt cgtcagcgac gtcgtagcgc
     1201 tcgtcgtggg gtttctgctg cacaccgaac atgccgatct cccgctcgtt ccagcccgct
     1261 acgatgttca ggcagaacct gcccccggag atctgatcca cggttgccga ttccttcgcc
     1321 agccgcacgg gatgcacggt gggcacgtga caggttgaga agagcgcgat ccgcttggtg
     1381 atcgccgcca gcgcgcttgc cagcgtgaac ggctcccagc tgcggtggtt gaagttcacc
     1441 gcgccgccca tgcccttcca ccgcgccacg ggtatcagcg catcgaaccc cgcttgctcg
     1501 gcggccagcg ccacctggcg ggtctcgggc cagctcactt ccagggtgcc tggagcacgg
     1561 gtcatcgtgc acccaccgct gacgttgatg ccgaacacgg cgagcttgaa ggggtgctcg
     1621 tcgctgcgca gcgggttgac gggcgagctg gtcatcgcgt tgcgcggagc aggcgacgcg
     1681 cgttctcggt gtacggcgcg gcgtcgtgac ggacgtctcc cggctgctgg tcccagcccg
     1741 caaaattggt gccgaacaca aggtgatcgg ggttcacctt tttgtgcaga agggccagag
     1801 cgtccggatc gtgcacgtga acgtcgaacc acagacgctg gagcagagcc tcgaacgcgc
     1861 cgtcctggcg cagcgagtcg gatgcccagg ggcgcttttt cgccgcgctc gccatccttc
     1921 cgaaggcgta tcccgtggcc ccgccaccat gactcaagca gacgtccagc ttcggatgac
     1981 gctccagcac cccgccgtag atgagcgtgc aggtggcaat cgtttcctgc gccgcgaagc
     2041 cgatgatgat gtccagatcg aactgcttga ggttgggatc ccccggcggg ccatcgatgc
     2101 ccgcgggggc ggggtggatg aaaagaggaa cgtccagctc gatgagcttt tcgtagaaag
     2161 gatcgagcgc ggggtcgttc agcggccgtc cgaagtcggt gccgatgtag ggaccgtaca
     2221 tgccgagctc gcgcaccgcc cgctccgtct cctcgatggc gaagtccacg tcctgcatgg
     2281 gcagcgcggc aaacgcgccg agacggtctg cccgctcgcc gacgatggca gacagggtgt
     2341 cgttgtggat cctgcagaaa tccctcgcgg cgctgccatc gatgaagtga aagtaggtga
     2401 gcgggttggg tgacaggacc tggtagtcga ttccggccgc gtccatggcg tcgaggcgca
     2461 gatccgggtc catgaacggg ctgccgcggt agcgcacgcc gtgcagctcg tagttgccca
     2521 ctcggaagcg cggggcagcc tcgcgaccga gctccgggcc gtgctcgccc gcggccccca
     2581 tggtctcttc cagaacggcg tgggcatgga cgtcgatggt ctgggcgctc gcaaggctca
     2641 tgtgtcgttt cctcgggctg ctgaagtggg ttcactcacc gaaaccagag cgatgatgga
     2701 cccggtcgga tggtaagatc aacgtcatga cgagttctga ttggggtcgc gtgcggcccg
     2761 ccgacatacc ctggaagcag ttcgacgatc ctcacggacg accgaccacc ccggttcgcg
     2821 ttctcaaagg gggcgacccc gccgttctcg aggtccggtt cccgccgggt tacgttgccg
     2881 ggctgcactg gcacccatac gacaccctct acatcgtcac tcgaggatcg atgcagatag
     2941 gtgatgaagg tgtgtatcgc ccgggagaca ttcgctgggt acgggcgggg cacgtttacg
     3001 ggccggaggc cgccggcgcc gaaggcgtgg actttttcct ggtgagcctc ggcggcgaga
     3061 tcgagcttaa ctgggccgac ctggtcgacg tgccggagcg gttgcgcgaa cgactggaga
     3121 cctcgggcga gccgtggggg cgggtaaacc ttcaagaggt gccgtggcag gacttcgagg
     3181 atcccgccgg acgacccacc cagccggttc aggttctcct ggacgatgat ccctacgtcc
     3241 tgcgaacccg attctcaccg gattacgttg ccggcgacca ctggcacgat ttcgacacgc
     3301 tttacttcat cacgtccggc tcgatgcggt tcggcgatga gggctggtac gagcctggtg
     3361 aagtccgctg ggtgcggggc ggtcacagct acggacccga agagcctgga gaaggcgggg
     3421 tggagttcat cctcgtcagt tgcggcggcc ccgtcaatct gcactggtcg gatctggaac
     3481 cgccg
//
LOCUS       DBNHOX010000130         4161 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_74501, whole genome shotgun sequence.
ACCESSION   DBNHOX010000130 DBNHOX010000000
VERSION     DBNHOX010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_74501"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..1134
                     /locus_tag="AC16UZ_02815"
     CDS             268..1134
                     /locus_tag="AC16UZ_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR6245642.1"
                     /translation="MSRIVKAAVIQLANQADTHEPCETHRRAMTEAHLPYIEQAGNAG
                     VQMLCFQEIFTGPYFCPSQDSKWYGLAEAIPDGPTTQLMCEYAKKYGMVIIVPIYEEE
                     MTGVYYNTAAVIDADGSFLGKYRKNHIPQVAGFWEKFFFRPGNLGYPVFTTAYGKVGV
                     YICYDRHFPEGARCLGLNGAEIVFNPSATVAGLSRHLGELEQTAHAAANGYFMAAINR
                     VGEEAPWNIGRFYGSSYFADPRGQIVAQASETESEMLIADLDFGQIRTVRDTWQFYRD
                     RRPETYDEIVEA"
     gene            1137..2537
                     /gene="hydA"
                     /locus_tag="AC16UZ_02820"
     CDS             1137..2537
                     /gene="hydA"
                     /locus_tag="AC16UZ_02820"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HIR6245643.1"
                     /translation="MSILIKNGRVITAVDDYCADIFIEHEVVSLIGRQLEVAADTVLD
                     ARGKFLFPGGVDPHTHLDMPFGGTTSADDFETGTRAAAHGGTTTLIDFAIQTKGESTL
                     AALETWHAKAEGKTAIDYGFHMIITDLEDDRLWEMRHLADEGVTSYKLFMAYPGVLYV
                     DDGTIFRAMRKAGDDGTVICMHAENGIVIDEIVKHALARGDTAPRFHGLTRPTRMEAE
                     GTHRAIAIAEVAGAPVYIVHLSCADALEQVTIARSRGVPVSAETCPQYLFLDHSYYEQ
                     EGFEGAKYVMTPPLRERWNQDELWRGLRFGDLQSIATDHCPFCFKEQKELGIDDFSKI
                     PNGAPGVENRMALVYNGGVHQGRISLNKFVELTSTAAAKTFGLFPRKGTIAVGSDADI
                     VIFDPQRVETISVNNPCTHHMRVDYNTYEGIEVHGFPETVISRGRVIVDNCAYTGKKG
                     DGRFMRRGLYGGLRAL"
     gene            2534..4015
                     /locus_tag="AC16UZ_02825"
     CDS             2534..4015
                     /locus_tag="AC16UZ_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014218.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HIR6245644.1"
                     /translation="MSAPSVPAGPAAGRFDPQLTNADLRPTEAADRRWGAGTYAALWV
                     AMAINIPSYMIAASLIEGGMSWKQAMWTVLLGNLIVLVPMALSGHAGTRYGIPFPVFA
                     RASFGVRGANIAGFLRAIVACGWFGIQTWIGGAAIYTIVLILWPGAAELPTALPAFLG
                     TSLVQFLCFMAFWCMNIWLIWKGLESIRILENLAAPFLLACGAALLWWAYQAADGFGP
                     MLRAQARFDSSAEFWALFFPSLTAMVGFWSTLCLNISDFTRHASSQRAQVIGQAAGLP
                     TTMALFAFIGVAVTSATIVIFGEAIWDPVVLVRRFDSPVTVFFAMIAVVIATLSTNAA
                     ANVVAPANTFSNMWPEKIDFKRGGYITGLVGILMMPWKLLADPSGYIFTWLIGYSALL
                     GPVVAIILVDYFLVRKTRLELDQLYRLDGIYAYRRGYNLKALWALLLGVAPNVPGFLN
                     QVGWMSRESAPFLVSLYSYAWFIGLFISGLAYWLMMRGSHARP"
     gene            4002..>4161
                     /locus_tag="AC16UZ_02830"
     CDS             4002..>4161
                     /locus_tag="AC16UZ_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidine dehydrogenase"
                     /protein_id="HIR6245645.1"
                     /translation="MPGRDVSAGRLSSERIQANFGELQPALTQLQAVDEAARCLYCYD
                     APCTEACPT"
BASE COUNT          717 a         1394 c         1298 g          752 t
ORIGIN      
        1 tatgtcggtt tgctggccga gcgctggttc tttttcgccc aggcgaacca cccgcagaac
       61 ctctattacc agacggtcta ggcgagcaca gagacagggg ctccggccag cagcggacga
      121 acacaagccc ttttttcgtg atgtgcaagg cagggctgcg cgtgcccgcc gggcgccgcg
      181 ctccaccctg ctgctgccgc atttcccatg tggtaacgat tggcgaacat ggtactatcg
      241 agatcaatcc atcagggtga attcatcatg tcacgcatcg tcaaggcggc cgtgattcaa
      301 ctggccaacc aagccgatac ccacgagccg tgtgaaaccc atcgacgggc aatgaccgag
      361 gcccatctcc cctacatcga gcaggccggg aatgccggtg tgcagatgtt gtgcttccag
      421 gaaattttca ccgggccgta tttctgcccc tcgcaggaca gcaagtggta cggcttggcg
      481 gaagccatac ccgatggccc caccacgcaa ttgatgtgtg agtacgcgaa aaagtacggc
      541 atggtcatca tcgtgccgat ctacgaggag gaaatgaccg gcgtctacta caacaccgcc
      601 gccgtgatcg acgccgacgg cagcttcctc ggcaaatacc gcaagaacca cattccccag
      661 gtcgcggggt tctgggagaa attcttcttc cgtccgggca acctgggcta cccggttttc
      721 accaccgctt acggcaaggt gggtgtgtac atctgctacg accggcattt cccggaaggc
      781 gcccgctgcc tcggtctgaa cggcgccgag atcgtgttca acccctcggc caccgtggcc
      841 gggctgagcc ggcacctggg ggaactggag cagacggcgc acgccgcagc caacggctat
      901 ttcatggccg ccatcaatcg cgtgggagaa gaagcgccct ggaacatcgg ccgtttctat
      961 ggctcatcct acttcgcgga cccgcgcggg cagatcgtgg cccaggccag cgaaacggaa
     1021 tccgagatgc tgatcgcgga cctcgacttc ggccagattc gaacggtgcg cgatacctgg
     1081 cagttctacc gcgaccggag gccggaaacc tacgatgaga tcgtggaagc ctagccatgt
     1141 ccatcctgat caagaacggg cgggtcatca cggcggtcga tgactactgc gcagacattt
     1201 tcatcgagca cgaagtcgtc tcgctgatcg gccgccagct ggaggtcgca gccgatacgg
     1261 ttctcgatgc gcgcggcaaa ttcctgtttc ccggcggtgt cgacccgcac acccacctgg
     1321 acatgccgtt cggcggcacc acctcggccg acgacttcga gaccggcacc cgcgccgcgg
     1381 cccatggcgg caccacgacg ctgatcgact tcgccatcca gaccaagggc gaatcgacgc
     1441 tggccgcgct cgagacctgg catgccaagg ccgagggcaa gaccgcgatc gactacggtt
     1501 tccacatgat catcaccgat ctcgaggacg accgcctgtg ggagatgcgc cacctggccg
     1561 acgagggcgt gacgtcgtac aaactgttca tggcctaccc gggcgtgctg tacgtcgacg
     1621 acggcacgat ctttcgcgcc atgcgcaagg ccggggacga cggcaccgtc atctgcatgc
     1681 atgcggaaaa cggcatcgtc atcgacgaga tcgtcaagca cgcgctggcc cgcggcgaca
     1741 ccgcgccgcg ctttcacggc ctcacccggc cgacgcggat ggaagcggaa ggcacgcacc
     1801 gggccattgc catcgccgag gtggccggcg cgcccgtgta catcgtgcat ttgtcctgcg
     1861 cggacgcgct ggagcaggtg accatcgccc gcagccgcgg ggtgccggtc tccgccgaga
     1921 cctgccccca gtacctgttc ctggaccaca gttattacga gcaggagggg ttcgagggcg
     1981 ccaagtacgt catgacgccg cccttgcgcg agcgctggaa ccaggatgaa ctctggcgcg
     2041 ggctgcgttt cggcgacctg caatccatcg ccaccgacca ttgcccgttc tgcttcaagg
     2101 agcagaagga gctgggcatc gacgatttct cgaagatccc caacggcgct ccgggggtgg
     2161 agaaccgcat ggcgctggtt tacaacggtg gcgtgcacca ggggcgcatt tcactcaaca
     2221 agttcgtgga attgacctcc acggccgcgg ccaagacctt cggcctgttc ccgcgcaagg
     2281 gcaccatcgc ggtcggcagc gatgccgaca tcgtgatatt cgatccgcag cgggtcgaga
     2341 ccatcagcgt caacaatccg tgcacccacc acatgcgggt cgactacaac acctacgagg
     2401 gcatcgaagt gcacggcttc cccgagaccg tcatctcgcg cgggcgggtg atcgtcgaca
     2461 attgcgctta caccggcaag aaaggcgatg gacgcttcat gaggcgcggc ctgtacgggg
     2521 ggctccgggc cctgtgagcg cgccctccgt ccccgccggg ccggccgcgg gccggttcga
     2581 cccgcaactg accaacgccg acctgcgtcc gaccgaggcc gcggaccgca ggtggggcgc
     2641 cggcacctac gcggcgctgt gggtggccat ggccatcaac atccccagtt acatgatcgc
     2701 cgcgtcgctg atcgagggcg gcatgagctg gaagcaggcc atgtggacgg tgctgctggg
     2761 caatctcatc gtgctggtgc ccatggccct gtccggccat gccggcaccc gctacggcat
     2821 tccgttcccg gtgttcgccc gcgcctcgtt cggcgtgcgg ggcgccaaca tcgccggctt
     2881 tttgcgcgcc atcgtcgctt gcgggtggtt cggcatccag acctggatcg ggggcgccgc
     2941 catctacacc atcgtgctca tcctctggcc gggggcggcc gagctgccga cggcgctgcc
     3001 cgcgttcctg gggaccagcc tggtccagtt cctctgcttc atggcgttct ggtgcatgaa
     3061 catctggttg atctggaaag gcctcgagtc catccgcatc ctggaaaacc tcgccgcccc
     3121 gttcctgctg gcgtgcggcg cggccctgct gtggtgggcc taccaggcgg ccgacggctt
     3181 cggccccatg ctccgggcgc aggcccgctt cgacagctcc gcggaattct gggcgctgtt
     3241 cttccccagc ctcacggcca tggtggggtt ctggtccacc ctgtgcctga atatttccga
     3301 tttcacccgc cacgcctcca gccagcgcgc acaggtcatc ggccaggccg ccggattgcc
     3361 caccaccatg gccctgttcg ccttcatcgg ggtggcggtc accagcgcga cgatcgtgat
     3421 attcggcgag gcgatctggg acccggtggt gctggtgcgc cgcttcgact cgccggtgac
     3481 ggtgtttttc gccatgatcg cggtcgtcat cgccacgctg tcgaccaacg cggcggccaa
     3541 cgtggtggcc ccggccaaca ccttctccaa catgtggccc gaaaaaatcg acttcaaacg
     3601 gggtggttac atcacggggc tggtcggtat cctgatgatg ccgtggaagc tgctggcgga
     3661 tcccagcggc tacatcttca cctggctgat cggctactcg gccctgctgg gcccggtggt
     3721 ggcgatcatt ctggtggatt acttcctggt ccggaagacc cggctggaat tggaccagct
     3781 gtatcgcctc gatggcatct acgcctaccg ccgcggctac aacctcaagg cgctctgggc
     3841 cctgctgctc ggggtggcgc ccaacgttcc gggcttcctc aaccaggtcg gctggatgag
     3901 ccgcgagagc gcgcccttcc tggtgtcact gtattcctat gcctggttca tcgggctgtt
     3961 catttccggc ctggcctact ggctgatgat gaggggttcc catgcccggc cgtgacgtct
     4021 ccgccggccg cctgagctcg gagcggatac aggccaactt cggcgagctg caacccgcgc
     4081 tgacccagct gcaggccgtc gacgaggcgg cccgctgcct ttattgctac gacgcgccat
     4141 gcacggaagc ctgcccgacc g
//
LOCUS       DBNHOX010000131         2237 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_460587, whole genome shotgun sequence.
ACCESSION   DBNHOX010000131 DBNHOX010000000
VERSION     DBNHOX010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_460587"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1690
                     /locus_tag="AC16UZ_02835"
     CDS             <1..1690
                     /locus_tag="AC16UZ_02835"
                     /inference="COORDINATES: protein motif:HMM:NF019229.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR6245646.1"
                     /translation="LELGQWQHVIVVYNGAVKRIFVNGVAEPTLQEKTDAIDSNGEGA
                     AIGGDADGTSGFNGLIDEVVLFDEAIGADQAQRLYASFAPPLILSVFEDVSVDAGFTG
                     PGANGQAAAFADANADGLVDLYLTLGSDPEQAVLQDRYFHNYDGTFVEEALTRAIEDA
                     DGGSYGAVWADLDNDGDYDLVNASAYTDLGEAGGVAANNNVYENDGTGNFLDVTALGA
                     VADAANARRSRGVTAHDADGDHDLDLFSIPLAADAGSNEAYLNDGSMVFSANLNAVLT
                     TTPASRGLIDTDFDGDGDVDILAASSPGAFAILRNDGAGTFVSVAPSDPTLGIEDDAE
                     DGISAADVDVDGDLDLLLVSDGSAHLYLRQSSGPYVKQQSFQGRDGYMGGFADIDHDG
                     DEDLIFAGDSVAYLNDGGGAFRPGPSIPMTGISDPRAIAFGDIDDDGDLDFVVTDDDG
                     ESRLIRNDLAQGNWLKVDLRSPQGQAGAFGARAYVYPPGGMPDEPRCESPVGEELIGM
                     RESRGNHGYLAQDDPVLHFGLGEHDPVDVVVCFLDGSETTVTAVPAGQTLQIRP"
     gene            1988..>2237
                     /locus_tag="AC16UZ_02840"
     CDS             1988..>2237
                     /locus_tag="AC16UZ_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245647.1"
                     /translation="MGHLQASTIRGFALALGTALLIGCSGSSKQTASEEPAPAETASS
                     TQEQSPPPAEAEPAPEEEAPAPAPVSVSLSAADDMVASG"
BASE COUNT          387 a          714 c          742 g          394 t
ORIGIN      
        1 cctcgaactg gggcagtggc agcacgtcat cgtcgtctac aacggggccg tcaagcgcat
       61 tttcgtcaac ggcgtggcgg agcccacgtt gcaggagaaa acggacgcca tcgactccaa
      121 cggcgaaggt gccgccatcg gcggggatgc ggatggcacg tccggcttca atggcctgat
      181 cgacgaggtg gtgctcttcg acgaggccat cggcgcggac caggcccaac ggctatacgc
      241 ttccttcgca cccccgttga tcctctcggt cttcgaggat gtgtcggtgg atgcgggctt
      301 cacgggcccc ggggccaacg gccaggccgc agcgtttgcc gatgccaatg cggatggcct
      361 ggtggacctg tacctgacgt taggctcgga tcccgagcag gcggttctac aggaccggta
      421 cttccacaac tatgacggta cgttcgtcga ggaggctctc acccgcgcca tcgaggacgc
      481 cgacggtggc agctacgggg cggtctgggc agatctcgac aacgacggcg attacgatct
      541 tgtcaatgcg agcgcgtaca cggatctcgg tgaggccggc ggggttgccg ccaacaacaa
      601 cgtctatgag aacgacggca ccgggaattt cctggacgtc accgccttgg gtgccgtcgc
      661 cgacgccgcg aacgccagac gctcgcgcgg cgtcaccgcc cacgacgcgg acggagacca
      721 tgacctggat ctgttcagca tccccctggc ggcggatgcg ggaagcaacg aggcctacct
      781 gaacgacggt tccatggttt tttcggcgaa cctgaacgcg gttctgacga ccacgccggc
      841 cagccgcggg ttgatcgaca cggatttcga cggggacgga gacgtcgaca tcttggcggc
      901 gagttccccg ggcgcgttcg ccatcctccg caacgacggt gccggtacgt tcgtcagcgt
      961 ggcgccgtcc gacccgaccc tcggaataga ggacgatgcg gaggacggca tctcggccgc
     1021 cgacgtggac gtcgacggtg atctggatct gctgctcgta tccgatgggt cggcgcacct
     1081 ctacctgcgc cagtcgtcag ggccgtacgt caagcagcaa tcatttcagg gccgcgatgg
     1141 ctacatgggg ggcttcgccg acatcgacca tgacggcgac gaggatctga tctttgcggg
     1201 cgacagcgtt gcctacctta acgacggtgg tggcgcgttc cgccctggac cgagcatccc
     1261 catgaccggc atatccgacc cgcgcgccat tgccttcggc gatatcgacg acgacggtga
     1321 tctcgacttc gtcgtcaccg acgacgacgg agagagccgt ctgatcagga acgacctggc
     1381 tcaaggcaac tggctcaagg tcgatcttcg atccccccag ggtcaggcgg gtgccttcgg
     1441 ggcccgcgcc tatgtttatc cgcctggcgg gatgccggat gagcctcgct gtgaaagccc
     1501 agtgggtgag gagctgatcg gcatgcggga gtcgcgcggc aaccacggct acctggccca
     1561 ggacgatccg gtgctccact tcggtctcgg cgagcatgat ccggtggacg tggttgtctg
     1621 ctttcttgat ggttccgaga ccacggtgac agccgtccct gccggccaga ctcttcagat
     1681 caggccctga taccgccccc ctcgcggcag gctcaccgag tcgagccgag ctgctggcgg
     1741 ctggtgattc cggcctgcag aacgtatgtg atcctttcgc tcagggcgtc gggacacgca
     1801 tcccaaatcc ttcgactccc gccggtgatg ttgatcagtt cgcactccgc gatggtcgaa
     1861 ttgtctaaat tccgctcacg cttcctcggt tacccatcga gaccggctgg agaggctcgg
     1921 gtcgaacagg tgcggaagga ccctcccagg gagcgatgca gttcgaggga aagaggattt
     1981 agggaccatg ggacacttgc aggcttccac catcaggggt ttcgcattgg cgctgggcac
     2041 ggcgctgctc atcggctgct cggggtcgtc caagcagacg gcatccgagg agcccgcgcc
     2101 ggccgagacc gcgtcgtcga cccaggagca gagcccgccg cctgccgagg cggagccggc
     2161 gcccgaagag gaagcccctg ctccggctcc tgtgtccgtc agcctgagcg cggcggacga
     2221 catggtggcc agcggcg
//
LOCUS       DBNHOX010000132         3350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_653315, whole genome shotgun sequence.
ACCESSION   DBNHOX010000132 DBNHOX010000000
VERSION     DBNHOX010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_653315"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            447..581
                     /gene="rpmH"
                     /locus_tag="AC16UZ_02845"
     CDS             447..581
                     /gene="rpmH"
                     /locus_tag="AC16UZ_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR6245648.1"
                     /translation="MKRTFQPSNLKRKRTHGFRSRMSTRGGRQVINARRAKGRKRLSV
                     "
     gene            523..945
                     /gene="rnpA"
                     /locus_tag="AC16UZ_02850"
     CDS             523..945
                     /gene="rnpA"
                     /locus_tag="AC16UZ_02850"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR6245649.1"
                     /translation="MAARSSTRGAPKVANASPSEAGDRAAASIHSFPRRRRLTRSRDF
                     DRVLKNAEVRRRSGPLRLTAVANTMQSARLGMIVGKRAVGRAHERNRMKRVIRDEFRR
                     RHAELADMDMVILVMAELENRELRAHLARLFESEVQKR"
     gene            945..1184
                     /gene="yidD"
                     /locus_tag="AC16UZ_02855"
     CDS             945..1184
                     /gene="yidD"
                     /locus_tag="AC16UZ_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR6245650.1"
                     /translation="MLRNAPRRLLQAGIRVYQWTLSPYLGRQCRFYPTCSHYAHEAIE
                     EFGAVRGSVLAARRLGRCHPFHPGGVDLVPTQEEG"
     gene            1191..2921
                     /gene="yidC"
                     /locus_tag="AC16UZ_02860"
     CDS             1191..2921
                     /gene="yidC"
                     /locus_tag="AC16UZ_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR6245651.1"
                     /translation="MLPTEVLRIVLLIALGVVGYLLILQWNEDYMQRPAAPDYAAEPS
                     PSAAEPALTAAPDLPVGAELPAGTGPSDIPDESLIGQDGAETQPALSVPMPDIGERLV
                     VVRTPLLHVWIDRLGGDVVRVALPKFPVDLDRPDVPFVLLDNGLDRTYVAQSGLIGPD
                     GIDAGGDRPLYATQAQSYTLEAGELVVELSTLRDGLEVAKRYMFRSDDYLMEVSYQVR
                     NLTGRTISARMFGQLKRDRKEPYVDTSIALGPQPYLGAAFTTAENRYEKLDFDDLDEG
                     RFRTTEQGGWVAFLQHYFLSAWIANPEETNSYYGQRRPDGTYMFGFSSPVSQVASGAL
                     GEWAASLYVGPKDQKRLEEIAENLNLTVDYGFLWWLAVPLFYLLDWLHGFVGNWGVAI
                     ILLTLLVKLVLYPLSATSYKSMANMRRVAPQMKRLQERHADDRQKLSQEMMALYKKEK
                     VNPLGGCLPMLLPMPIFIALYWVLFESVELRQAPFALWINDLAAMDKYFVLPLLMGAS
                     MYLQQLLSPAVGDPMQVRMMRLMPIMFTVLFLFFPAGLVLYWLVNSVLSIAQQWYVIR
                     QTERAYAAKS"
     gene            2899..>3350
                     /locus_tag="AC16UZ_02865"
     CDS             2899..>3350
                     /locus_tag="AC16UZ_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR6245652.1"
                     /translation="MPTPPKADSLGLDVDTIAAVATPVGSGGVGIVRISGPRARAIGE
                     TVSARSLHPREAFYCRFRNAQGEVVDDGIVLYFAGPASFTGEDVVELQGHGGPVVVQM
                     VLGIVLEAGARQARPGEFSERAYLNGKMDLAQAEAIADLISSSSTAAAR"
BASE COUNT          628 a         1038 c         1032 g          652 t
ORIGIN      
        1 aagcatttcc ggcaggtttt ttggtatcga ctgcaaattc agaaacttac gtctgccgtt
       61 ggccatggtg agctcgcgat tcgagcttgc ggcgatgtga gcaactcgtt gaagaacctg
      121 tggatagttt tccgccgctc gctttacccc cgatgatccc gcccgggtcc tcaacgggcc
      181 ccaagcatat ccacaccctt gcagcgagga aaacaacttg ccggggcaaa gacttacgcc
      241 cgatatcaac agatgctgcc gtctcaacaa gcaacaacta caaattatct atatatattt
      301 cttactatga gaaggagtga tgtcgcagct gcgttgacac tcaaggggcc tgatccataa
      361 aatcccgctc cctcgcgatc ccggccgccg actaaagcgc gccaaagggg cgggagcttc
      421 ggtaagtcag caacggaaac ccgaacatga agagaacgtt tcagccaagc aatctaaagc
      481 gcaagcgcac tcacggcttc cgttcgcgta tgtccactcg aggtggccgc caggtcatca
      541 acgcgaggcg cgccaaaggt cgcaaacgcc tctccgtctg aggcaggcga tcgcgcagct
      601 gcttcgatcc actcatttcc gcgaaggcgc cggctaacca gatcgcggga cttcgaccgc
      661 gtcctgaaga acgccgaggt gcgccgccgg agcgggccgt tacggctgac ggcggtagcg
      721 aatacaatgc agagcgcccg cctggggatg atagtcggca agcgggccgt cggcagggcc
      781 cacgaacgca accgaatgaa gcgcgtgatc agggacgagt ttcgtcgacg ccacgcagag
      841 ctggccgaca tggacatggt cattctggtg atggctgagc tggagaaccg agagcttcgc
      901 gcccatcttg cgaggctgtt tgagtcggag gtgcaaaaaa gatgatgctt cgcaacgcac
      961 cgcgccgctt gcttcaggcg ggcatccgtg tgtatcagtg gacgttgagt ccgtacctgg
     1021 ggcgccagtg ccgcttctac cccacctgct ctcactacgc ccacgaggcc attgaagagt
     1081 tcggcgccgt ccggggctcc gtccttgccg cgcgaaggct ggggcgctgc cacccgtttc
     1141 atcccggcgg cgtcgatctg gttccgaccc aagaggaggg ttgatcagcc atgctgccaa
     1201 cggaagtcct caggatcgtt ctcctcatcg cgctgggggt ggtgggttac ctgctcatcc
     1261 tgcagtggaa cgaggattac atgcagcgtc ctgctgctcc cgactacgcg gccgaaccct
     1321 cgccatccgc tgcggagcct gcgctgacgg ccgcgccgga tttgcctgtt ggcgcggaac
     1381 tccctgcggg tacgggacca tccgacatcc cggacgaatc gttgatcggt caagacggtg
     1441 ctgaaaccca gccggccctc agcgtgccga tgccggacat cggggaacgg ctcgtcgtcg
     1501 tcaggacgcc gttgctgcac gtctggatcg accggctggg cggggacgtg gtgcgcgtcg
     1561 cgttgcccaa gtttcccgtg gacctggatc ggcccgacgt cccgttcgtg ctgctcgaca
     1621 acgggctcga tcgcacctat gtggcgcaga gcgggctcat cggcccggac ggcatcgatg
     1681 cgggcggcga tcgaccgctg tatgccaccc aggctcagag ctacaccctg gaggcggggg
     1741 agctcgtagt ggagctctcg acgctccggg acggtctcga ggtcgccaag cgctacatgt
     1801 tcagaagcga cgactacctc atggaagtgt cctaccaggt tagaaacctg accgggcgca
     1861 ccatctcggc tcggatgttc ggccagctca aacgggatcg caaagagccg tacgtggata
     1921 cctcgattgc tctcggaccc cagccctatc tgggggctgc gttcaccacc gcagagaacc
     1981 gctacgagaa gctcgatttc gacgatctcg acgaaggccg cttccggacc accgagcagg
     2041 gcggttgggt ggccttcctg cagcactact tcttgagcgc ctggatagcc aacccggagg
     2101 aaaccaacag ttactacggt caacgccgac ccgatggcac ctacatgttc ggattttcca
     2161 gcccggttag ccaggtggcc agcggggcct tgggcgagtg ggcggccagc ctgtatgtgg
     2221 gccccaagga tcagaagcgc ctggaagaga tcgctgaaaa tcttaatctc accgtggact
     2281 acgggtttct ctggtggttg gcagtccctc tcttctacct cctcgactgg ctgcacggtt
     2341 tcgtcgggaa ctggggagtc gccatcattc tgctcaccct gctggtcaag ctggtgctct
     2401 acccgctctc ggcaacgagc tacaagtcca tggcgaacat gcggcgcgtt gcccctcaga
     2461 tgaaacggtt gcaggagagg catgcggacg accgtcagaa gctgtcccag gagatgatgg
     2521 ccctatataa gaaagagaag gtcaacccgt tgggtggatg ccttcccatg ctcctgccca
     2581 tgcccatatt catcgctctg tactgggtgc ttttcgagag tgtggagctc agacaggcgc
     2641 cctttgccct ctggatcaac gacctcgcgg ccatggacaa gtatttcgtc ctgcctcttc
     2701 tgatgggcgc gagcatgtat ctgcagcagc tgctgagccc cgcggtcggt gatcccatgc
     2761 aggtccgcat gatgcggctc atgcccatca tgttcaccgt gctgtttctc ttcttcccgg
     2821 ccgggctggt gctctactgg ctcgtcaaca gcgtgctgtc catcgcccag cagtggtacg
     2881 tcataaggca gacggagcgt gcctacgccg ccaaaagctg actctctcgg cctggacgtc
     2941 gacacgatcg cggccgtggc gacgcctgtc ggttccgggg gcgtgggcat cgtgcgaatc
     3001 tccgggcccc gagcccgcgc catcggagag acggtctcgg cccgtagcct ccacccacgc
     3061 gaggccttct attgcaggtt ccgaaacgcc cagggtgagg tcgtcgatga cggcatcgtt
     3121 ctctactttg ccggaccggc ctcctttaca ggagaggacg tggtggagct gcaggggcac
     3181 ggcgggccgg tagtcgtgca gatggtcctt ggtatcgtgc tggaggcagg ggcccggcag
     3241 gcacgccccg gggagttctc cgaacgggcc tatctgaacg gcaagatgga tcttgctcag
     3301 gccgaggcca ttgccgatct gatttccagc tcgtccacgg cagccgctcg
//
LOCUS       DBNHOX010000133         2646 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_781512, whole genome shotgun sequence.
ACCESSION   DBNHOX010000133 DBNHOX010000000
VERSION     DBNHOX010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_781512"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="AC16UZ_02870"
     CDS             <1..323
                     /locus_tag="AC16UZ_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245653.1"
                     /translation="VGLALLAAGVALRPPGGIVEITIFSGSLYAVCFFPAILFGLHWR
                     GGSAQAVLASMVSGVLVLVGWLLAGGREFAHEVFPAMLVSCLSYAWVANVTRPALEHW
                     PPEV"
     gene            complement(333..644)
                     /locus_tag="AC16UZ_02875"
     CDS             complement(333..644)
                     /locus_tag="AC16UZ_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6245654.1"
                     /translation="MIRLYGMGRSNYFSIAKACLVERGMAFEKVATQPSAENAYLAKS
                     PMGKIPCIETERGFLSEAYAIVERIFGKDLLDSIPRARDLLALVGQQPSIQRVEAEKA
                     G"
     gene            complement(641..1645)
                     /locus_tag="AC16UZ_02880"
     CDS             complement(641..1645)
                     /locus_tag="AC16UZ_02880"
                     /inference="COORDINATES: protein motif:HMM:NF016086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HIR6245655.1"
                     /translation="MSGHTLSSTAKRTYWLGRYLERTESTARLIAENGNLMIDLPARL
                     PHSWSPLISITGSGATFATLYEEPTERNVTRFLIGDLRNHGSILSSLGWARENVRTLR
                     GIIPRQAVEYIIELHLYARDHLSGALSRSRRAEGLTVVPALIQQIEGFMSANMLHDAH
                     WNFWRLGNFVERADMTTRIIDVGTDNPLQTADAPGPFTDLQWRSVLRSLNATQSYLTV
                     RQEPIHQSAVVGFLIGNRSLPRSLTYSLESLRNGLRTLPRSDRALRAVNRLRRFLQSS
                     EIENLEGPELHQFLDGCQVQLAEIHQQIDRTYFEFHPRRIPQRADRSADTQPTRSDST
                     "
     gene            complement(1642..>2646)
                     /locus_tag="AC16UZ_02885"
     CDS             complement(1642..>2646)
                     /locus_tag="AC16UZ_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HIR6245656.1"
                     /translation="WSHVCGSDLVRDSDGRVYVLEDNLRVPSGVSYMLENREVMKRVF
                     PELFEDYVIHPVDDYPARLASCLRTLTSVKSPQIAVLTPGIYNSAYFEHAYLANAMAA
                     QLVEGSDLVVGKDDYVYMKTIDGLVRVDVIYRRIDDLFLDPDVFKPDSMLGVPGIVRA
                     WRAGNVALANAPGCGVADDKVIYTYVPDLVRYYLAEEPLLPNVPSYRCVDKEQRDYVL
                     DNLDELVVKPANESGGYGMLIGPKASRAERNRFARLIRANPRNYMAQPMLKLSTAPTL
                     VDEHAEPRHLDLRPFILSGASSYVTMGGLTRVALRKGSTVVNSSQGGGSKDTWVVDLE
                     "
BASE COUNT          419 a          802 c          905 g          520 t
ORIGIN      
        1 tggtcggcct tgcgctgctg gctgccgggg tagcgctgcg gccgccgggc ggcatcgtcg
       61 agatcacgat attttcgggc agtctctacg ccgtctgctt cttcccggcc atactgtttg
      121 gtctgcactg gcgcggaggt tcagcccagg cggtgcttgc gtccatggtc agcggcgtgc
      181 tcgtgctggt gggctggctg ctcgccggcg gaagggagtt cgctcacgag gtgttccccg
      241 ccatgctggt ctcgtgtctc agctatgcgt gggtcgccaa cgtgacccgc ccggccctcg
      301 agcactggcc ccccgaggtc tgacgtcgtc gctcagcccg ctttctcggc ctctacgcgc
      361 tgaatgctgg gttgctggcc aacgagcgcc agcaggtcac gggcccgcgg tatcgagtcg
      421 agcagatcct tgccgaagat tctttcgacg atggcgtatg cctcggaaag gaagccccgt
      481 tccgtctcga tgcaggggat cttgcccatg gggctcttgg cgaggtaggc gttctccgca
      541 gacggctgag tggcgacttt ctcgaacgcc atgcccctct ccaccagaca cgccttggcg
      601 atgctgaaat agttgctgcg tcccatgccg tagagcctga tcatgtgctg tcgctcctgg
      661 ttggttgagt gtctgcggaa cgatccgccc gttggggtat gcgccgtgga tggaactcga
      721 agtaggtccg gtcgatctgc tggtgtatct ctgccagctg tacctggcag ccgtccagaa
      781 actggtgaag ttcagggccc tcgaggttct cgatctcgct gctctgcagg aacctgcgca
      841 gcctgttgac ggccctcagg gcgcggtcgc tgcgaggcag cgtgcgcaga ccgttgcgca
      901 ggctctccag cgagtaggtc agggaccgcg gcagcgagcg attgccgatg agaaatccga
      961 ccacggcgct ctgatggatg ggctcctggc gtacggtcag gtagctttgc gtggcgttca
     1021 gagagcgcag cacgctgcgc cattgcaggt cggtgaacgg ccccggcgcg tccgcggtct
     1081 gcaaagggtt gtcggtgccc acgtcgatga tgcgggtggt catgtcggct cgctccacga
     1141 agttgccgag ccgccagaaa ttccagtggg catcgtgcag catgttcgcg gacatgaagc
     1201 cttcgatctg ctggatcagc gcggggacca cggttagccc ttcggctcga cgggagcgtg
     1261 acagggcgcc gctcaagtgg tcgcgcgcgt acagatgcag ctcgatgatg tactccacgg
     1321 cctgccgcgg gatgatcccg cgcagggtcc tcacgttttc cctggcccag cccagcgatg
     1381 acaggatgct gccgtgattg cgcaggtcgc cgatcaggaa gcgggtgacg ttgcgctccg
     1441 tgggctcttc gtagagcgtg gcaaacgtcg ctccgcttcc ggtgatgctg atcagcggcg
     1501 accagctgtg ggggagccgt gcgggcagat cgatcatcag gttgccattc tccgcaatca
     1561 gccttgcggt gctctcggtg cgctccaggt agcgtcccag ccagtaggtg cgctttgccg
     1621 tgctcgacag cgtatggccg ctcattccag gtccaccacc caggtgtctt tactgccgcc
     1681 gccctgagac gagttgacca cggtcgagcc cttgcgcagc gcgacccggg tcaatccgcc
     1741 catggtcacg tagctgctgg cgccggagag aatgaagggt ctcaagtcca gatgccgggg
     1801 ttcggcgtgc tcgtcgacca gcgtgggcgc ggtggagagc ttgagcatgg gctgggccat
     1861 gtagttgcgc gggttcgcgc gaataagcct ggcgaaccgg ttccgctccg cccgtgacgc
     1921 cttgggcccg atcagcatgc cgtagcctcc ggactcgttc gcgggcttca ccaccagctc
     1981 gtccagattg tcgagcacgt agtcacgctg ctccttgtcc acgcaccggt aggaaggcac
     2041 gttgggcagc aaaggctcct cggccaggta gtagcggacg aggtcgggga cgtaggtgta
     2101 gatcaccttg tcgtctgcca cgccgcagcc gggcgcgttg gcaagcgcca cgttgcccgc
     2161 gcgccaggcg cgcacgatgc cggggacgcc gagcatggaa tccggtttga agacgtccgg
     2221 atccagaaag agatcgtcga tgcgacggta gatgacgtcg acgcggacca ggccgtcgat
     2281 ggtcttcatg tacacgtagt cgtccttgcc gacgacgagg tcggagccct ccacgagctg
     2341 cgcggccatg gcgttggcga ggtaggcgtg ctcgaagtag gccgagttgt agatgccggg
     2401 cgtgagcacg gcgatctgcg gggatttcac cgaggtaagc gtgcgcagac agctcgcgag
     2461 cctggctgga tagtcgtcca cggggtggat cacgtagtct tcgaaaagct ccggaaagac
     2521 gcgcttcatg acttcccggt tttccagcat gtaggaaacg ccggaaggca cacggaggtt
     2581 gtcctcgagc acgtagacgc ggccgtccga gtcgcggacc agatcggagc cgcagacgtg
     2641 tgacca
//
LOCUS       DBNHOX010000134         1501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_139003, whole genome shotgun sequence.
ACCESSION   DBNHOX010000134 DBNHOX010000000
VERSION     DBNHOX010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_139003"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="AC16UZ_02890"
     CDS             <1..669
                     /locus_tag="AC16UZ_02890"
                     /inference="COORDINATES: protein motif:HMM:NF028456.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245657.1"
                     /translation="PVILLTDGYIANASELWPLPDFDHYEPIETHKPDPSTDPRHSVF
                     SRDPKTEARLWATPGDKRFVYRVGGIEKDIHTGNISYEAQNHQEMTNMREAKVASVAS
                     FIPDQQVDSGTDAGLAVVAWGSTYGTIYQAVRECVAEGLAVGHIHLRHLNPLPPNLPT
                     LLAQFDRILVPELNNGQLATLLRDKLVLPVEQHNQVNGQPFAVADVKQAIARYAPKRI
                     KEAG"
     gene            673..>1501
                     /locus_tag="AC16UZ_02895"
     CDS             673..>1501
                     /locus_tag="AC16UZ_02895"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR6245658.1"
                     /translation="MAELTFKDYATDQEVRWCPGCGDYSILKAVQRALADAGAAKEQT
                     VFVSGIGCSSRLPYYVDTYGFHTIHGRAPAVATGVKLANPDLDVWVITGDGDALSIGS
                     NHLMHALRRNVDLNILLFNNEIYGLTKGQYSPTSQLGTRSPSTPAGSVDSPVSACVFA
                     LGCNARFVARSVDTNQKMLPGLLVEAKNHKGASFVEILQNCIVYNDGVFSAFTEKAVA
                     ADGQILCEHGKPLLFGKEGNRGLRLTPGKLELEVVTVGENGITEADILIHDETNRPLA
                     "
BASE COUNT          298 a          503 c          456 g          244 t
ORIGIN      
        1 ccggtgattc ttctgaccga cggctacatc gccaacgcct ccgagctttg gcccctgccg
       61 gacttcgatc actacgagcc gatagaaacg cacaaaccgg atcccagcac cgatccacgc
      121 cattccgttt tcagccgcga tccgaagacc gaggcccgtc tgtgggcaac gcccggggac
      181 aaacgcttcg tctaccgggt cggcggcatc gagaaggaca tccacaccgg caacatctcc
      241 tacgaagctc agaatcatca ggaaatgacc aacatgcgcg aggccaaggt ggccagcgtc
      301 gccagcttca tccccgatca gcaggtggat tcgggaaccg atgccgggct ggccgtagtg
      361 gcctggggat ccacgtacgg aacgatctat caggcagtca gagagtgcgt cgccgagggg
      421 ctggccgtgg gtcacataca tctgcggcac ttgaacccgc tgccgccgaa cctgccgacc
      481 ctgctggcgc agttcgaccg gatcctcgtt cccgagctga acaacggcca gctcgcgacc
      541 ctgctgcgag acaagctggt cctcccggtg gagcaacaca atcaggtcaa cgggcagccg
      601 tttgccgtgg cggacgtcaa gcaggcgatc gcgcgatacg cgcccaagcg catcaaggag
      661 gcaggttgaa tcatggcgga gctgactttc aaagactacg cgactgacca ggaagtgcgc
      721 tggtgcccgg gatgcggtga ctactccatc ctcaaggccg tgcagcgggc gctggctgat
      781 gctggagccg ccaaagagca gacggtattc gtgtcgggca tcggctgctc gtcacgcctt
      841 ccctactacg tggacaccta cggttttcac accattcacg gcagagcgcc cgccgtcgcg
      901 accggggtga agctggccaa ccccgacctc gacgtctggg tgatcacggg tgacggcgac
      961 gccctctcca ttggcagcaa tcacctaatg cacgcgcttc gccgcaacgt ggacctgaat
     1021 atcctgctat tcaacaacga gatctacggc ctgaccaaag ggcagtactc gccgacctcg
     1081 cagctcggca cgcgctcgcc ctcgacgccg gccggctcgg tcgattcgcc ggtctcggcc
     1141 tgcgtgttcg cgctgggctg caacgcgcgc ttcgtcgcgc gctcggtcga caccaaccag
     1201 aagatgctgc ccggcctgct ggtcgaggcg aagaaccaca aaggcgcgtc gttcgtcgag
     1261 atcctgcaga actgcatcgt gtacaatgac ggcgtctttt ccgcctttac cgagaaggcg
     1321 gtcgccgccg acgggcagat cctgtgcgag cacggcaagc cgctgctgtt cggcaaggag
     1381 ggcaaccgcg gcctcaggct cacccccggc aagctggaac tcgaggtggt cacggtcggc
     1441 gagaacggca tcaccgaggc cgacatcctg atccacgacg agaccaaccg gccgctggcg
     1501 c
//
LOCUS       DBNHOX010000135         2929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1231222, whole genome shotgun sequence.
ACCESSION   DBNHOX010000135 DBNHOX010000000
VERSION     DBNHOX010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1231222"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="AC16UZ_02900"
     CDS             complement(<1..1155)
                     /locus_tag="AC16UZ_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6245659.1"
                     /translation="MRTTYLTAGAIALAIVVWLLSGQMASGDPVRHQTLAENNAEALA
                     RTQDKAPTRVRARVMNATPQFQHVVLRGKTESKRVVEVRSETSGRVIERPVERGAYVT
                     QGDLLCRLAMDDREAALAEARESLNQARIEYEGSLKLKQRGFQSDTAIAQTKARLAAA
                     EAKLARSALEVSRTEIRAPFDGVVETIHQDVGDYVNVSAPCVTVVDADPMLLIGRVAE
                     KDVHRLLLDQNATGLLSNGTTVTGPVTFIGQQSDPATRTYAVEIQIPNPERAIRSGIT
                     TEIRIPVAETMAQKVSPAVFTLDDAGHIGIRIVNADYRVELHPVTIIREAPDGVWVSG
                     LPNVATVITVGQELVVAGEMVEVDFEPAATLPAAAPAERAVAPEADGPDSG"
     gene            complement(1359..>2929)
                     /locus_tag="AC16UZ_02905"
     CDS             complement(1359..>2929)
                     /locus_tag="AC16UZ_02905"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR6245660.1"
                     /translation="EIDGRLLVDGGVSNNLPVDVALAMGADVIIAVDLSSPLRGRDEL
                     GSVLEVTDQLTSILTRRNTTEQIARLRPQDVLIVPELGGVTAIDFDLAATTIPSGYSA
                     AIAARPRLASLALPEEPFEQHVAGLRRQEVTTIDVAEVVLTTDSRVDADLLLARTGLQ
                     PGTMEVAQIEAAVDELYGMELFSQVPYAYQHGELHINPIRRDWGPNYLQFGLALEDNL
                     EGRNHYNLGVAFTATELNAKAGEWRTELGIGERPRLVTEFFQPFGPRGRWYVNPSVGV
                     SAFNRGVFETDNLVAEYQIRQVAGTLATGYAFGSRAEARIGLSLAEGTRRRLVGDPTF
                     EKTSADSGMAFVEFRYDSLDDLYFPQRGSTLQARWTLSEESLGAEVDYRAAELAASRA
                     LSFGRHTLVVGGEFATVYEGEAPFHEQYNLGGFLNLSGLEVDQKLGQHRGIGRVVYYY
                     RWSSNPVLPVYLGMSAEAGQVWIEDDEIGFDNLEAAGSLFLGLDSPIGPVFLAGGMAE
                     GGNKSIYVFLGQPF"
BASE COUNT          487 a          926 c          988 g          528 t
ORIGIN      
        1 tccgctgtcg gggccgtcgg cttccggcgc gacggcgcgc tccgcggggg cggcggccgg
       61 cagggtggcg gcaggctcga agtctacctc caccatctct ccggccacga ccagctcctg
      121 gccgacagtt atcacggtgg cgacgttggg aaggccggac acccacacgc cgtctggcgc
      181 ttctcggatg atggtcacgg ggtgaagctc gacgcggtag tcggcgttga cgatgcgaat
      241 gccgatgtgc cccgcatcgt cgagcgtgaa gacggcaggg ctgactttct gggccatggt
      301 ctcggccacg gggatgcgaa tctcggtggt gattccggag cggatcgccc gctccggatt
      361 gggaatctgg atctccaccg cgtaggtgcg ggtagcggga tcgctctgct ggccgatgaa
      421 ggtgacgggg ccggtgacgg tcgtcccgtt actcaacagc ccggtcgcgt tctggtccag
      481 aaggagccgg tgcacgtctt tctcggcgac ccgcccgatc aaaagcatgg ggtcggcatc
      541 gacgacggtg acgcagggcg cgctcacgtt gacgtagtcg cccacgtcct ggtgtatggt
      601 ttccaccacc ccgtcgaagg gcgcgcggat ctccgtgcgg gagacctcca gagcgcttcg
      661 ggcgagcttg gcttcggcgg ctgctagacg cgccttggtc tgggcgatgg cggtatcgga
      721 ctgaaagccc cgctgcttga gcttcaggct cccttcatac tcgatccggg cctgattgag
      781 ggattcgcgg gcctcggcca gtgcagcctc gcgatcgtcc atggcaagcc ggcacaggag
      841 atccccctgg gtaacgtacg caccgcgctc gactggtctc tcgatcacgc ggccggacgt
      901 ctcggagcgg acctcgacga cgcgctttga ttcggttttc cccctcaaga cgacgtgctg
      961 aaactgcggc gtggcgttca ttacccgggc gcgcaccctg gtcggtgcct tgtcctgggt
     1021 ccgcgcgagg gcttccgcgt tgttctctgc cagcgtctga tgcctgacag ggtcgccgct
     1081 cgccatctgg cccgaaagca gccagacaac gatggccagc gcgatggcgc ctgcggtgag
     1141 atatgtggtt cgcactttgg tctccgatga gcaagtggtg ttactaaagc acccaatacg
     1201 ttacctaaag taacaaactg ccggtggtat ttccatgggc cgctgcacaa tcaaggatgc
     1261 tcggcgggcc tggaagctgt cagggttaaa ggtagcgatg gcggtgtgta ggcggcgtga
     1321 aaacgccgcg cgatgtggct ctaaaggcgg ccttgcggct aaaacggttg cccgaggaag
     1381 acgtagatgg acttgttccc cccttcggcc atgcccccgg cgagaaaaac gggccctatg
     1441 ggactgtcca gaccaagaaa caggctgccg gccgcttcca ggttgtcgaa gccgatctcg
     1501 tcgtcctcga tccagacctg gccggcttct gccgacatgc caagatagac gggcagaacg
     1561 gggttcgaag accagcggta gtagtacacg acccttccaa tgccccggtg ctgaccgagc
     1621 ttctggtcga cctccaggcc ggagagattg aggaatccgc ccagattgta ctgctcgtga
     1681 aagggggctt cgccttcgta gaccgtggcg aattccccgc cgaccaccag ggtgtggcgc
     1741 ccgaagctca gcgctcgaga agcggcaagc tccgcggcac ggtagtcgac ctccgccccc
     1801 agagattcct ccgaaagcgt ccacctggcc tgcagggtgc ttccacgctg cggaaagtac
     1861 agatcatcca ggctgtcgta gcgaaactcg acgaaggcca ttcccgagtc cgcggacgtc
     1921 ttctcgaagg tagggtcgcc gacgaggcgc cgccgcgtac cctcggccag gctgagcccg
     1981 atgcgggcct cggcccggct gccgaatgcg taccccgtgg ccagggtgcc tgcgacctgc
     2041 cggatctggt actcggcgac caggttgtcc gtctcgaaca cgccgcgatt gaaggcgctt
     2101 acgcccactg aggggttcac gtaccagcgt ccccgggggc cgaagggctg gaagaactcg
     2161 gtaaccaggc gcggacgctc gccgattccg agctccgttc gccactcgcc ggcctttgcg
     2221 ttgagctccg tggcggtgaa ggcgacgccc agattgtaat gattgcgtcc ttccaggttg
     2281 tcctcgagcg cgagtccgaa ctgcagatag ttcggacccc agtcccgcct gatggggttg
     2341 atgtggagct ccccgtgctg atacgcgtag ggcacctggg agaaaagctc catgccgtag
     2401 agctcgtcca cggcggcttc gatctgcgcg acctccatgg tgccgggctg cagtcccgtc
     2461 cgggccagca gaagatcggc gtccacccgg ctgtcggtgg tcagcaccac ctccgcgacg
     2521 tctatggtgg tgacctcttg ccggcgcaga ccggcgacgt gctgctcgaa gggctcttcc
     2581 ggcaacgcca gtgatgccag gcgaggccgg gcggcgatgg ccgcgctgta gccggacggg
     2641 atggtggttg ctgccaggtc gaaatcgatg gcggtcaccc cgccgagctc gggcacgatg
     2701 agcacgtcct gtgggcgcaa ccgggcgatc tgctccgtgg tgttgcggcg ggtgagaatg
     2761 ctggtcagct ggtcggtcac ctccaggacg gagccgagct cgtcgcggcc gcgcagggga
     2821 gagctcagat ccacggcgat gatcacgtcg gcacccatgg ccagggccac gtccaccggc
     2881 aggttgttgg aaacgccgcc gtccacgagg agacgcccat cgatctccg
//
LOCUS       DBNHOX010000136         1716 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_396177, whole genome shotgun sequence.
ACCESSION   DBNHOX010000136 DBNHOX010000000
VERSION     DBNHOX010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_396177"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /gene="guaB"
                     /locus_tag="AC16UZ_02910"
     CDS             <1..722
                     /gene="guaB"
                     /locus_tag="AC16UZ_02910"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR6245661.1"
                     /translation="GHSQRVLDRISWIKQRYPDVNVIGGNVATADGAKALLSAGVDAV
                     KVGIGPGSICTTRMVTGIGVPQITAIAEAVAVADSEGVPVIADGGVRYSGDIAKAIAA
                     GASVVMVGGLLAGTDEAPGEVELYQGRTYKSYRGMGSLGAMTESNGSSDRYFQEAEGD
                     HRKLVPEGIEGRVPYRGPVGPIVHQLMGGLRASMGYTGSNDIETMRTQPRFVRVSAAS
                     MVESHVHDVAITKEAPNYPVS"
     gene            723..>1716
                     /gene="guaA"
                     /locus_tag="AC16UZ_02915"
     CDS             723..>1716
                     /gene="guaA"
                     /locus_tag="AC16UZ_02915"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR6245662.1"
                     /translation="MADKYDIHDQRLLIIDFGAQYTQLIARRVREAGVYCEIHPFDVP
                     EDFIREFAPRGVILSGGPESVTFADTPRIPSIVFELGVPILGICYGMQAMAEQLGGSV
                     EASDVHEFGHAEIEIVETAELLGTVRDTAGGHLPVWMSHGDRVVRTPPGFEVTASTPS
                     APVAAMEHREKRMYGVQFHPEVTHTQRGEEMLAHFARTLCRCDGLWTAANIVEDAIAH
                     ARDLVGDERVVLGLSGGVDSSVVAALLSRAIGDQLTCVFVDNGLLRKGEREEVERAFA
                     PTNALALNLVSVPAAEEFLGRLAGVVDPEAKRKVIGNTFIEVFDREAHKISGVSW"
BASE COUNT          295 a          520 c          597 g          304 t
ORIGIN      
        1 acgggcactc ccagcgggtg ctggaccgca tcagctggat caagcagcgc tatccggacg
       61 tcaacgtcat cggcggcaac gtggctaccg cggacggcgc gaaagcgctt ctttccgccg
      121 gcgtggatgc ggtcaaggtc ggcatcggcc ccggctccat ctgcaccacg cgaatggtta
      181 ccggcattgg tgttccgcag atcacggcca tcgccgaagc ggtggcggtt gccgactcgg
      241 aaggggtgcc cgtcattgcc gatggaggtg tccgttactc gggcgacatt gccaaagcca
      301 tcgcggccgg ggccagcgtg gtgatggtcg gcgggctgct cgccggcacc gatgaagcgc
      361 ccggagaggt agagctctat cagggcagaa cctacaagtc ctatcgcggc atgggctcgc
      421 tgggtgcgat gaccgagtcc aacgggtcca gcgatcggta cttccaggaa gcagaggggg
      481 atcatcgcaa gctcgttccc gaggggatcg aagggcgcgt gccttaccgt gggcctgtgg
      541 gccccatcgt tcatcagctc atgggcgggc tcagggcgtc gatgggctat accggctcga
      601 acgatatcga gaccatgcga acccagcctc gcttcgtgcg agtgagcgcg gccagcatgg
      661 tggagagtca cgttcacgac gtggcgatca ccaaagaagc gccgaactat cccgtcagct
      721 gaatggcgga caaatacgac atacatgatc agcgcctcct gatcatcgat ttcggtgccc
      781 agtacaccca gctgattgcc cgccgggtgc gcgaggccgg cgtttattgc gagattcacc
      841 ccttcgacgt cccggaggat ttcatacgcg agttcgcgcc gcggggggtg atcctctctg
      901 gcggcccgga gtcggtaacg tttgcggaca cgccgcgcat cccgtccatc gtgttcgagc
      961 tgggggtgcc catcctcggc atctgttacg gcatgcaggc catggcagag cagctgggcg
     1021 gttccgtgga agcgtccgac gtccatgagt tcggccacgc agaaatcgag attgtcgaga
     1081 cggccgagct gctcggcacg gtgcgtgaca ccgccggcgg gcatctgccg gtctggatga
     1141 gccacggcga ccgggtggtg aggacgcctc cgggcttcga ggtcaccgcc agcaccccgt
     1201 cggcgcccgt ggctgcgatg gagcatcgcg aaaagcgcat gtacggcgtg cagtttcatc
     1261 ccgaggtgac gcacacccag cggggcgaag agatgctcgc ccacttcgcg cggacgctgt
     1321 gtcgctgcga tggcctgtgg acggccgcca acatcgtcga ggacgccatc gcccacgccc
     1381 gcgacctggt tggagacgaa agggtcgtgc tggggttgtc cggaggcgtg gattccagcg
     1441 tggttgctgc tctgctctcc cgggccatcg gggatcagct gacgtgcgtg ttcgtggaca
     1501 acggcctgct tcgcaagggc gagcgggaag aggtggagcg tgcttttgcg ccgaccaacg
     1561 ctctggcgct caacctggtc agcgtgccgg ccgcggaaga gtttctgggg cgcctggccg
     1621 gtgtcgtgga ccccgaggcg aaacgcaagg tcatcggtaa tacgttcatc gaggtcttcg
     1681 atcgtgaagc gcacaagatc tccggggtga gctggc
//
LOCUS       DBNHOX010000137         3772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1617478, whole genome shotgun sequence.
ACCESSION   DBNHOX010000137 DBNHOX010000000
VERSION     DBNHOX010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1617478"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="AC16UZ_02920"
     CDS             <1..657
                     /locus_tag="AC16UZ_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6245663.1"
                     /translation="REASDGHEAGGFFVGQGRMLIALPRSDEWQIGYVFPKGDFKTLR
                     ESGIQAFRDSIAATAPWLAGRTDVIHGWERVHLLTVSSDRLPTWHRPGLLCIGDAAHV
                     MSPVGGVGINAAIGDAVEAANVLTRPLLDAVAPGEAALAEVQRRREVPTRIIQGLQAR
                     IQDTVVSRALRDEEFHLPLPARLILATPGLRNLPPRIFAQGIKPLRLEALPRTEHQRT
                     "
     gene            654..1520
                     /locus_tag="AC16UZ_02925"
     CDS             654..1520
                     /locus_tag="AC16UZ_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6245664.1"
                     /translation="MTGLRLGVVGDLHTHWDDIDVAQFNATRYDLLFFTGDLGGGSVE
                     SSLRVARTISRLDVETLVMPGNNDTGDIHQLAAELSHRSGLKRLTGIRQGAGDAIKPI
                     RLCGYSSHRYRTPAADVTLIAARPHSMGGPTLTFADHMSATYGISSLEASAMRLKELV
                     DAAETADLVFLSHNGPTGLGGEPADMWGCDFKPDGGDWGDPDLREAIAHAVATGRRVL
                     AVVAGHMHLKTKCGQERPWRREHDGILYVNAARVPRIYSGSDDVMRHHVSLHIHEQGI
                     EIDELEVPQGLG"
     gene            complement(1517..2005)
                     /locus_tag="AC16UZ_02930"
     CDS             complement(1517..2005)
                     /locus_tag="AC16UZ_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245665.1"
                     /translation="MNIRTDAIVRLRDHLLSSDLGAAGQVPDEQPGDAVRLRVEPFAE
                     TMFLVMLADAEQAVSEHDALVVAVDMLTDGLMGREAVDALLAEFAERVRREGREDCVA
                     RIGARLSADRDDREMAFTLAAVIALADDRVAVEENEVLDLMRTYYGISDRRMEAILGT
                     LV"
     gene            complement(2002..3429)
                     /locus_tag="AC16UZ_02935"
     CDS             complement(2002..3429)
                     /locus_tag="AC16UZ_02935"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6245666.1"
                     /translation="MDHAEFEARLGKVLALRVGRVRSLRSAERLSGGASQETYRLTVD
                     TADGEELMAMRRAPGGGLDVEVAPPTLATEALLMRSARAAGVPEPEVYYVLEPEDGLG
                     EGFVMEWLEGEALGARIVRARELDGVRPQLAYECGRVMAKIHAIDLDRAGLRERLETL
                     TPEELLHRSWDRYKDFPTPQPMIDFTARWLLDNMPATFDMALVHNDFRNGNFMVDANG
                     IVAVLDWEVAHIGDPMRDLGWICTNSWRFGRSDRPVGGFGEYADLFRGYEDESGVAVD
                     PERVKFWEVMGSFWWAVGCLGMAEHYRTGPDKTVERPAIGRRSSECQVDCVNLIIPGD
                     VELVAGTDLEDCTDMPRVDELLVSVRDYLRADVMAATSGRTNFLARVAGNSLDIVLRE
                     LATGAEARFREQVRLEALLGRSGDLDALRWELVHRLRSGEMALDDVALTDHLRTTVVN
                     QIAIDQPRYSGFKTALARAGIEVPE"
     gene            complement(3432..>3772)
                     /locus_tag="AC16UZ_02940"
     CDS             complement(3432..>3772)
                     /locus_tag="AC16UZ_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6245667.1"
                     /translation="FPLVELQTQCEMLRALIHKTAWEMDTYGAFSVSDKVSMCNYWSN
                     RLCCESADRAMQVHGGLGYSRHKPFEHIYRHHRRYRITEGAEEIQMRRVAGYMFGFMS
                     QRAPKGVRES"
BASE COUNT          672 a         1324 c         1168 g          608 t
ORIGIN      
        1 cgggaagcat ccgacggcca cgaggcgggc ggtttcttcg tcgggcaagg ccgcatgctc
       61 atcgcgctgc cccgttcgga cgagtggcag atcggctacg tgtttcccaa gggagacttc
      121 aagacgctca gggaatccgg aatccaggcg ttccgggact ccatcgcggc aaccgcgccc
      181 tggctggccg gacgcaccga cgtcattcac ggctgggaaa gggtgcacct gttgacggtg
      241 agctctgacc ggctgccgac gtggcaccgg cccgggctcc tctgcatcgg ggacgccgcg
      301 cacgtcatgt ccccggtggg gggtgtgggc atcaatgcgg ccattggcga cgccgtcgag
      361 gcagcgaacg ttctgactcg acccctgctg gacgccgtcg ctcccggcga agcggccctg
      421 gccgaggttc agcggcgccg ggaggttccc acccggatca ttcagggctt gcaggcgcgc
      481 atacaggaca ccgtggtgtc gcgcgcactg agagacgagg agttccatct gccgcttccc
      541 gcccggctga tcctcgccac gccggggctg agaaaccttc ccccaaggat tttcgcgcag
      601 ggcatcaagc cgctaagatt ggaagccctc ccccgcacgg agcatcaaag aacatgacgg
      661 ggctgcgttt gggcgtggtc ggcgacctgc acacccactg ggacgatatc gacgtggcgc
      721 agttcaacgc gacgcgctac gacctgctgt tcttcacggg cgacctcggt ggcggcagcg
      781 tcgagagcag cctgcgggtg gcgcgaacga tctcgcgact cgacgtggaa accctggtca
      841 tgcccggaaa caacgatacc ggcgacattc accagctggc tgccgagctt tcccaccgct
      901 caggcctgaa aaggctcacc gggatccgcc agggcgccgg cgacgcgatc aagcctattc
      961 gcctttgcgg gtacagcagc catcgctatc gcacccccgc tgcggacgtg acgctgatcg
     1021 cggcccggcc ccacagcatg ggcggcccga ccctgacctt cgccgaccac atgtctgcca
     1081 cgtacggtat cagcagcctt gaagcgtctg ccatgcgcct gaaggaactg gtcgacgcgg
     1141 ccgaaaccgc cgacctcgtg ttcctctccc acaacggacc cacgggcctg ggcggggagc
     1201 cggcagacat gtggggctgc gacttcaaac ccgatggggg agattggggc gatccggatc
     1261 tccgagaagc cattgctcac gccgtcgcga ccgggcgacg agtgctggcg gtggtcgcgg
     1321 gccatatgca tctcaagacc aagtgcggcc aggagcgccc ctggcgccgc gagcacgacg
     1381 ggatcctcta cgtcaacgcc gcccgggttc cgcggatcta ctccggcagc gacgacgtca
     1441 tgcgccacca cgtctcgctg cacattcacg aacagggcat cgagatcgac gagttggagg
     1501 tcccccaggg gctgggctaa accagggtcc cgagtatggc ttccatgcgt cggtccgaga
     1561 tcccgtagta ggtccgcatc agatcgagca cctcgttctc ttccaccgcg acacgatcat
     1621 ctgccagcgc gatgacggcg gcgagcgtaa acgccatttc ccgatcgtcc cggtccgcgc
     1681 tcaaccgagc tccgatccgc gccacgcaat cttctcttcc ttcccgacga acgcgctcag
     1741 cgaattcggc cagcagcgcg tccacggctt cgcggcccat cagaccatcg gtgagcatgt
     1801 cgaccgcaac caccagggcg tcgtgttccg aaacggcctg ttcggcatcc gccagcatca
     1861 ccagaaacat ggtctcggca aaaggctcta cccgcaggcg gaccgcatcg ccgggctgct
     1921 catcgggaac ttggccggca gccccgagat cgctggagag caggtgatcg cgcagccgca
     1981 caatggcgtc ggttcgaatg ttcattcggg cacctcgatt ccggctcttg ccagcgccgt
     2041 tttgaaacca gagtacctgg gctgatcgat ggcgatctgg ttcaccacgg tggtgcggag
     2101 gtgatccgtg agggcaacgt cgtcgagcgc catctcgccg gatcgcagcc ggtggacgag
     2161 ctcccagcgc agggcgtcca gatcccccga acgccccagc agcgcttcca gccggacctg
     2221 ctcacggaac ctcgcctcgg ctcccgtcgc caactctcgc agcacgatgt ccagggagtt
     2281 gccagccacc cgtgccagaa agttcgtccg cccgctcgta gccgccatga cgtcggcgcg
     2341 caggtaatcg cgcacgctca ccagcagctc gtcgacccgg ggcatgtccg tgcaatcctc
     2401 aagatccgtg cccgccacca gctccacgtc accaggaatg atcaggttga cgcaatccac
     2461 ctggcactcc gacgagcgcc ggccgatggc cggccgctcc acggtcttgt cggggccggt
     2521 acggtagtgc tcggccatgc ccagacagcc gaccgcccac cagaacgacc ccatcacctc
     2581 ccagaacttc acgcgctcgg ggtccactgc cacgccggac tcgtcctcgt agccgcggaa
     2641 cagatcggcg tactcgccga aaccacccac cggccggtcg ctgcgtccga aacgccagga
     2701 gttggtgcag atccagccga ggtcgcgcat gggatcgccg atgtgggcga cctcccagtc
     2761 gagcacggcg acgatcccgt tcgcgtccac catgaaattg ccgttgcgaa aatcgttgtg
     2821 gacgagcgcc atgtcgaacg tcgccggcat gttgtcgagc agccagcgcg ccgtgaagtc
     2881 gatcatcggc tgaggcgtgg gaaagtcctt gtagcgatcc cagctgcgat gcagcagctc
     2941 ctccggagtc agagtttcca gccgctccct gagcccggca cggtccaggt cgatggcgtg
     3001 aatcttcgcc atcactcgcc cgcactcgta agcgagctgc ggccgaacgc cgtcgagctc
     3061 gcgcgcccgg acgatgcgcg cgccaagcgc ttcgccctcc agccactcca tcacgaaacc
     3121 ttcgcccagg ccgtcctccg gctccagaac gtaatagacc tcgggctccg ggaccccggc
     3181 ggcgcgggca ctgcgcatca gcagggcttc ggtggccagc gtcggtggcg caacctccac
     3241 gtccaagccg cccccgggag cccggcgcat ggccatgagc tcctcgccgt cagccgtatc
     3301 gacggtaagc cgatacgtct cctggctggc tcccccggac aggcgctcgg cagagcgcag
     3361 cgagcggacg cgcccgaccc gaagagccag caccttgcca agccgcgctt cgaactcggc
     3421 gtggtccacc ttcagctttc cctgacaccc ttgggcgcgc gctgcgacat gaatccgaac
     3481 atgtatccgg ctacccgacg catctggatt tcctccgcgc cctcggtgat ccggtagcgc
     3541 cgatggtgcc gatagatgtg ctcgaaaggc ttgtgccgcg agtaccccag accgccgtga
     3601 acctgcatgg cgcgatcggc cgattcgcag cacagacggt tcgaccagta gttacacatg
     3661 gacaccttgt ccgaaacgga gaacgcaccg tacgtatcca tttcccaggc ggttttgtgg
     3721 atgagggccc ggagcatctc gcactgggtc tgcagctcga cgaggggaaa ct
//
LOCUS       DBNHOX010000138         3551 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_910647, whole genome shotgun sequence.
ACCESSION   DBNHOX010000138 DBNHOX010000000
VERSION     DBNHOX010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_910647"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1796
                     /locus_tag="AC16UZ_02945"
     CDS             168..1796
                     /locus_tag="AC16UZ_02945"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6245668.1"
                     /translation="MKDHDVKPLGRLLTAAAAAHPDRDAMVLVDERATYAELVSRAKR
                     AGRSLMALGVEPGDRVGILMANCLDFVDILFGTTLIGAVPVLYNARFKAREIAHVTAD
                     AGVKVVVTNDIVEQHTNYVELLRRSLPGLAEHDAIRTLALKAAPDLRTAVTLSSSSTA
                     GFLNRNAFYELGSTHPEEVVDERGARVRPEDVAVMIYTSGTTAMPKGCPLTHIVLQHA
                     GVVGGVERLGLIEGDVMWAPLPMFHTAFTQPLTGILHVGGTFLSMTHFDAAAALRMIH
                     DEQATVMFPAFPTITMQLLDHPSYTAQSFACVRVAINVGPPEELCAMQERMPHTTQIT
                     VFGMTETGGSAAMCERDDSLAHRTETSGFALPGNEIQIRDPDSGAELPAGERGEIVVR
                     GRGVFSGYHKAPEKTAESFYDGGWFRTGDLGVMDADGRVTFRGRLKDMLKVGGENVAA
                     VEVEGFLATHPAVNLAQVVAVPDDKYGEVPAAFVELAPGASASEQEIIDYCKDEIASF
                     KIPRFVRFVEEWPMGATKILKYELRERICSELGL"
     gene            complement(1926..2741)
                     /locus_tag="AC16UZ_02950"
     CDS             complement(1926..2741)
                     /locus_tag="AC16UZ_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6245669.1"
                     /translation="MSEGPATEQFGEFTTLERRGRVVVASFDRGDGLNALSSNLMREL
                     TALAGELADDTESSVVVLTGRGAFSAGADLKEPDAGASRTPTLLERRQALSIGPDLCA
                     AWERLEQITIAAVEGFCIGGGVALAVACDHRVAGSSAHFRLPEVPLGMNMSWQSNPRT
                     VALIGPSRAKLFTILGEKLAADTALEWGLVDEVVADGQALASAVSLADRYAAMPPLPL
                     RMTKQAINAATGALNYATSYMDRDQFLLTALSDDQKEGVRAFLEKRTPEFRGD"
     gene            complement(2969..>3551)
                     /locus_tag="AC16UZ_02955"
     CDS             complement(2969..>3551)
                     /locus_tag="AC16UZ_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR6245670.1"
                     /translation="VQLEMVQMNFNELLPALESGKVDLVISGMTATLQRNMRAAFVGP
                     YHITGKSILTRSETIAALGSNDLNREDLRIAALKGSTSEDFVRRVAPNAKLVATDTYD
                     EAIDQLMANKAELFVADAPRVMLEVMRWPDAGLTAAQRPLTIEPIGIAVPASDPLLLN
                     LVENYMAALESSGALEKLEEKWFKSGGWLVQLP"
BASE COUNT          643 a         1179 c         1137 g          592 t
ORIGIN      
        1 cggccaactc cgggaggagc tggtaacgct agcaaaaggg cgtcatttca cgggcggctg
       61 catgcgcgtc gccgctcaag cccttacact atcgtccggt tatgcttagc ggaaatatcc
      121 gaagtgcctg tgctttctca ccacgccgac gagaattcgc cttgcccatg aaagaccacg
      181 acgtcaaacc tctgggccgg ctattgaccg ccgccgcggc cgcacacccc gaccgcgatg
      241 ccatggtgct cgtggacgaa cgcgccacct acgcagagct cgtctcccgg gcaaagcgcg
      301 ccggccgctc gctcatggcg ctgggcgtgg aacccggcga ccgggtgggc atcctgatgg
      361 ccaactgtct ggacttcgtg gacatcctgt ttggcacgac gctcatcggc gccgtgcccg
      421 tgctctacaa cgcccgcttc aaggcccggg agattgcgca cgtcaccgcc gacgccggcg
      481 tcaaggtggt ggtcaccaac gacatcgtcg agcagcacac caactacgtg gagctgctcc
      541 ggcgctccct gccgggtctc gccgagcatg acgccatccg cacgctggcc ctgaaagcgg
      601 cgcccgatct gcgcacggcg gtgaccctct cgtcatcgag cacggccggc ttcctgaacc
      661 gaaacgcgtt ctacgagctg ggctcgacgc atccggagga ggtcgtcgac gagcgcggcg
      721 cccgggtccg cccggaggac gtcgccgtga tgatctacac ctcgggaacc accgccatgc
      781 cgaaaggctg ccctctcacc cacatcgtgc tgcagcacgc tggcgtggtc ggcggcgtgg
      841 agcggctcgg gctcatcgaa ggggatgtga tgtgggcacc gctgccgatg tttcacacgg
      901 cgtttaccca accactgacg ggcattctgc acgtgggcgg caccttcctc tccatgacgc
      961 acttcgacgc cgcggcggct ttgcgcatga ttcacgatga gcaggcgacg gtcatgttcc
     1021 cggcgtttcc gaccatcacc atgcagctgc tcgaccaccc ttcgtacacc gcgcaaagct
     1081 ttgcctgcgt acgcgtcgcg atcaacgtag ggcctcccga ggagctttgc gccatgcagg
     1141 aacgcatgcc gcacaccacg cagatcaccg tattcggcat gaccgagacg ggcgggtcgg
     1201 cggcgatgtg cgagcgcgac gacagcctgg cccatcgcac ggaaacctcc gggttcgcgc
     1261 tgccgggtaa cgagattcag atccgcgatc cggacagcgg cgccgagttg cctgccggcg
     1321 agcggggaga gatcgtcgtc aggggacgcg gcgtgttctc cggctaccac aaagcgccgg
     1381 agaagaccgc agaatcattc tacgacggcg gctggttcag aaccggcgac ctcggagtca
     1441 tggacgccga cggccgggtg acgtttcggg gccggctcaa ggacatgctc aaggtcggcg
     1501 gcgaaaacgt cgccgcggtg gaggtggagg ggttcctggc cacccatccc gccgtcaatc
     1561 tggcacaggt agtcgccgtt cccgacgaca agtacggtga ggtaccagcc gccttcgtcg
     1621 agctcgcgcc cggagcctca gcgagcgagc aggagatcat cgactactgc aaagacgaga
     1681 tcgcaagctt caagattccc cgattcgtcc gcttcgtgga ggagtggccc atgggcgcga
     1741 ccaagatcct gaaatacgag ctgcgcgagc gcatctgctc ggagctcggg ctctgacgag
     1801 cgtcgagcgg gtccccgtcg agggcggtct gtacggagct tccccccaaa gacaacactg
     1861 aggcgcggcc aggcagcgcc cggttgcctg gacagggctt tgaacccgcc ctgcccgcgc
     1921 ctggatcagt ctcctctaaa ttccggcgtt cgtttctcca gaaaggctcg cacgccctcc
     1981 ttctggtcat cgctcagcgc ggtgagcaga aactggtccc gatccatgta gctcgttgcg
     2041 tagttgaggg ccccggtggc ggcgttgatg gcctgcttgg tcattcgcag gggcagcggg
     2101 ggcatggccg cgtaccgatc ggccagcgaa acggctgacg ccagcgcctg cccgtcggcg
     2161 accacttcgt ccacgaggcc ccattccagc gcggtgtctg cggcgagctt ctctccgagg
     2221 atggtgaaaa gcttggcgcg ggagggaccg atcagagcca ccgtgcgggg attgctctgc
     2281 cagctcatgt tcatccccag cggaacttcc ggcagccgga agtgggcaga ggagccggcc
     2341 acccgatggt cgcacgcaac agcgagggca acgccacccc cgatgcagaa gccttccacc
     2401 gcagcaatgg tgatctgctc gagccgctcc caggcggcac agaggtccgg cccgatcgaa
     2461 agagcctgac gccgctcgag caacgtcggc gtccgggaag cgcccgcgtc gggttccttc
     2521 agatcggcac ccgccgaaaa cgcgccccga ccggtcagga cgacaacgga gctttccgtg
     2581 tcgtcggcaa gctcaccggc aagggccgtg agttcgcgca tcaggttgga ggaaagggca
     2641 ttcaagccat cgccgcggtc gaacgaagcg acgacgacgc ggccccgacg ttccagcgtg
     2701 gtgaactcgc cgaattgctc ggtggcgggc ccttcgctca tgttcgcctc cctggctggt
     2761 cctgcccgat cttaacaaaa gccgcccgag ccgagccccg gctccgcggt ttcagccgtc
     2821 ttcgctttgg gtcattgaca tcccgacgag cgcaaggatg acgggcacaa cgaggatcag
     2881 gttgggctga cggggcgagg atagggacat ggtccaggct cgcaccgaag gaatcggcgc
     2941 gaaaccagga ccacgggttc atctcaggtc acggcagctg aacgagccaa ccgccgctct
     3001 tgaaccactt ttcttcgagc ttctccaagg cgccgctcga ctcgagagcg gccatgtagt
     3061 tctccaccag gttcagcagc aacgggtcgc tggcgggaac cgcgatgccg atgggctcga
     3121 tggtcagcgg acgctgggcc gcggtcaggc ccgcatccgg ccaccgcatg acctcgagca
     3181 tgactcgggg agcatcggcg acgaacagct ccgccttgtt ggccatcagc tggtcgatgg
     3241 cctcgtcgta agtgtccgtg gccaccaact tggcattcgg agccacccgc cgcacgaaat
     3301 cctcgctggt cgagcctttc agcgcggcga tgcgcaggtc ctcgcggttc agatcgtttg
     3361 agcccagggc ggcaatggtt tccgagcgcg tcagaatgga cttgccggtg atgtggtaag
     3421 gcccgacaaa cgcggcgcgc atgttgcgct gcaacgtggc cgtcataccg gatatcacca
     3481 gatcgacctt gccggactcc aatgccggca gaagctcgtt gaagttcatc tgcaccatct
     3541 cgagctgaac a
//
LOCUS       DBNHOX010000139         2633 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_525116, whole genome shotgun sequence.
ACCESSION   DBNHOX010000139 DBNHOX010000000
VERSION     DBNHOX010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_525116"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1624)
                     /locus_tag="AC16UZ_02960"
     CDS             complement(<1..1624)
                     /locus_tag="AC16UZ_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024971.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR6245671.1"
                     /translation="MVNHPRIGSLRLVKLLMVLTPAAAFAAPADHPVFGQGQPANVSE
                     LPPGMLKERLESLPPTARQSALRWLQDFSFPAADAAHMSADDAGGIYYVDEAPDGVEE
                     AEGAAAEPPTSGALSAAETFALHSKPGASKVLFLDFDGHVIEGTAWNANSSRPDTYYA
                     RPFDTDGSPSSFGSAELETIHEVWHRVAEDFAAFDVDVTTEDPGSFGPSVGHVLVTKD
                     DDELGNAMPSAGAGGVAYVGVFGRSNYTYYQPALVYYDNLGNGHPPYVAEASSHEFGH
                     NLSLSHDGTSTTSYFQGLGSTSSFVSWAPIMGVGYYRQVTQWSNGEYADANNTQDDLS
                     IITGHLGPRGDDHGDDILSASLLVTDGAGTISHSNPQSDPGNFNPENKGIIDQQGDVD
                     VFGLQVGTGTVDITVTPAWDAFTRSMRRGANLDIQARLLDDTGTVIATSDPGDDTDAS
                     IQVQLDAGYYYLAVTGVGNSSVPYSDYASQGMYFISGQAVTGEPNGAPTANGDTAGTA
                     EDFAVDVAVLANDSDPDGDALSILSVSSPANGT"
     gene            1908..>2633
                     /locus_tag="AC16UZ_02965"
     CDS             1908..>2633
                     /locus_tag="AC16UZ_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245672.1"
                     /translation="MRKSRHTQWTVNPSRAGSQGLILAMIFSGFSLAGLQAFAAKPNP
                     ICGNGICEQRESKSCPLDCADDPGEPPEPPPSTGVPSCGCSGAGLSPAQAKAGRSYVR
                     DLKVNDPVSGEVTSPYEVRLPSSYDVNSPGGFPVLVYLHGWGGSFRSLPASAARHAKN
                     NGYILVTPTGYGDGGRNSWNGYRSARLPVCALGDGYLASDCDAGPPELDSANGPSTCV
                     DLDNAGHDYCYDSCRTLYGTGAEP"
BASE COUNT          420 a          804 c          939 g          470 t
ORIGIN      
        1 tggtgccgtt tgctggcgag ctgacggaca ggatcgagag cgcatcgccg tccggatcgc
       61 tgtcgttggc cagcaccgcg acgtccacgg cgaagtcctc ggccgttccg gccgtgtcgc
      121 cgttggcggt gggcgcgccg ttgggctcgc cggtgaccgc ctgccccgat atgaagtaca
      181 tgccctggct cgcgtagtcg gagtagggga ccgagctgtt cccgacgccg gtaacggcga
      241 ggtagtagta gccggcgtcc agctgaacct gaatgctcgc atcggtgtcg tcgccgggat
      301 cggacgtggc gatgacggtg ccggtgtcgt ccagcagccg ggcctggatg tccaggttcg
      361 cgccccggcg catgctgcgg gtgaaggcgt cccatgcggg cgtcaccgtg atgtcgaccg
      421 tgccggtacc gacctggagc ccgaagacgt cgacgtcgcc ctgctggtcg atgatgcctt
      481 tgttttccgg attgaagttg cccggatccg attgcgggtt gctgtgagaa atggtgcccg
      541 cgccatcggt gacgagaagg ctggccgaga ggatgtcgtc gccgtggtca tcaccacggg
      601 ggccgagatg gccggtgatg atggacaggt catcctgggt gttgttggcg tccgcgtact
      661 cgccgttgct ccactgggtc acctggcggt agtagcccac ccccatgatg ggtgcccacg
      721 agacgaagct cgaggtgctt ccgagtccct ggaagtagct ggtggtgctc gtcccgtcgt
      781 gggagaggct caagttgtgg ccgaactcgt gagaggaggc ttccgccacg tacgggggat
      841 gcccgttgcc caggttgtcg tagtagacga gcgcgggctg gtagtaggtg tagttcgagc
      901 ggccgaacac gcccacgtag gcgaccccgc cggcgcccgc gctgggcatg gcgttgccga
      961 gctcgtcgtc gtccttggtg acgagcacgt gcccgacgga cgggccgaag ctgcccggat
     1021 cctcggtggt tacgtccacg tcgaaggcgg cgaagtcctc cgccacccgg tgccagacct
     1081 cgtgaatggt ttccagctcc gccgagccga agctcgacgg cgaaccgtcg gtgtcgaacg
     1141 ggcgggcata gtaggtgtcg ggccggctgc tgttggcgtt ccaggccgtg ccttcgatga
     1201 cgtggccgtc gaagtcgaga aagagcacct tggaggcgcc gggcttgctg tgcagggcga
     1261 aagtctcagc cgcgctcaaa gccccgctgg tcggcggctc ggcggccgcg ccttcggctt
     1321 cctcaacgcc gtcgggcgct tcgtcaacgt agtagatgcc gccggcgtca tcggcgctca
     1381 tgtgagcggc gtcggccgcg gggaagctga agtcctggag ccatctcaac gcgctctggc
     1441 gggctgtggg gggcaagctc tcgagacgct ccttgagcat gccggggggc agctcggaga
     1501 cgttggccgg ttgtccttgg ccgaacacgg gatggtcggc cggggcggca aaggcggcgg
     1561 ctggggtcag caccatcagg agcttgacga gcctcagcga gccgattctg gggtgattga
     1621 ccacgggcaa tctccactgt ctggggtacg actatggtag ggagcggcat tgcccggcat
     1681 gtgcggatag gtgagccgaa tgcgccgtct tggtagcgca ggtaatgaga atgttggact
     1741 gtgacgtgct ccccattccc ccggaaaccg cggtgttcgg ggtgatggaa agggttgcgg
     1801 acggtcgatg taggaaagat gcaatccagg cgaaggagtg tctcggaacg gggcgttaat
     1861 tgagttagtc tagacagtaa attgacatta gcgacgagga ggtggacatg aggaaatccc
     1921 ggcacacgca atggacggtc aatccgagcc gggcaggcag tcagggcctg atcctcgcca
     1981 tgatcttttc cggattctcc ttagccgggc tgcaggcgtt cgccgccaag ccgaacccaa
     2041 tctgcggcaa cggcatctgt gagcagcgcg aatccaaaag ttgccccctg gactgcgcgg
     2101 acgatcccgg cgagcccccg gaaccgccgc cttccactgg ggtgccatcg tgtggctgca
     2161 gcggcgcggg gctctccccg gcccaggcaa aagccggccg gtcctacgtg cgtgatctga
     2221 aagtcaacga tcccgtctcg ggcgaggtca cgagccccta cgaggtgcgg ctgccgtcga
     2281 gctacgacgt caactccccc ggcgggttcc cggtgctcgt ctacctgcac ggctggggtg
     2341 ggagctttcg atccctgccg gcgtcggccg cccgtcatgc caagaacaac ggctacatcc
     2401 tggtgacccc caccggctac ggcgacggcg gccggaattc ctggaacggc tatcgcagcg
     2461 cccggctgcc cgtgtgcgcg ctcggggacg gatacctcgc ctccgactgc gatgccgggc
     2521 cgcccgagct ggactcggcg aatgggccgt ccacctgcgt ggatctcgac aacgccggcc
     2581 acgactactg ctacgacagc tgcaggaccc tctacgggac gggagccgag ccg
//
LOCUS       DBNHOX010000140         2943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_654004, whole genome shotgun sequence.
ACCESSION   DBNHOX010000140 DBNHOX010000000
VERSION     DBNHOX010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_654004"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="AC16UZ_02970"
     CDS             complement(<1..1109)
                     /locus_tag="AC16UZ_02970"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6245673.1"
                     /translation="MHDLIIRNATLVDGTGAPRREGDVVVDDGTIVAVAAKGSAPASA
                     ARVIDAGGDLLTPGFVDIHTHYDGQVCWDKQLTPSCWHGVTSIVMGNCGVGIAPVRPG
                     TEGELVALMESVEDIPGTALHDGIPWGWETFSEYLDAIDTPYVMDVGAQVPHVAIRHY
                     VMGERCYDDATPEDMTAMRDLTRTALEAGALGFSTSRFYGHVDKAGRLVPGTRASADE
                     MRAIAGAFEGLGYGTMEFVSDQLDDPAELAWIERITRATGCTVTPLTTAGFGPLWTLA
                     EKLGAEGFNLRPQVGARPASILMTLDGTINPMRQFPAYREIHERPLAERRAMLKDPAF
                     RQRVLTEEPILPRNADAVRFITDHEHMYVMDAALS"
     gene            1217..2017
                     /locus_tag="AC16UZ_02975"
     CDS             1217..2017
                     /locus_tag="AC16UZ_02975"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6245674.1"
                     /translation="MSEPVLKIFSYLPNPRVWKAQIAAELCGVAVDVVGDRPAALQNW
                     LWDFEARPLEDAERTANSPHARESRRGFSGLLFKTDAFLTAHPFGTVPAAFAPGGEPG
                     IFESNSILRAVARAGAANRQLYGGDGYQASRIDGFLDANLVFAREAQVYLLGMDEPQA
                     HVHERMAAAYQFYLAGVESALARSAFLAGDELSIADISFVCDFAQFLREGHYEDALEK
                     AGLSLISGNGPEDYPRSFHHLLTLSAEPAFAQHMGTYLDWYRRRVAAA"
     gene            complement(2033..2737)
                     /locus_tag="AC16UZ_02980"
     CDS             complement(2033..2737)
                     /locus_tag="AC16UZ_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009401404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6245675.1"
                     /translation="MAEQKVCVVTGVGPGTGAAMVRRFHEGGYRVAMIARTAERLEAL
                     AGEMPNAHAYPCDMADPAQIESTLARVADELGPPSIAIHNAVGGAFGDFLSIEPAQLE
                     ANFQVNVMGLLHLGRAVAPAMIEAGEGAIIVTGNTSALRGKAGFAGFAPTKAAQRILA
                     ESMARSLGPQHVHVAYIVIDAVIDLAWTRRRFADQPDGFFIQPASIAENAWYLAHQDP
                     SAWTFLSELRPHGENW"
     gene            complement(2730..>2943)
                     /locus_tag="AC16UZ_02985"
     CDS             complement(2730..>2943)
                     /locus_tag="AC16UZ_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245676.1"
                     /translation="RCRARRQAADPSGGQRADRVHGAGHRLYVMDWNINGASTAEWQH
                     GLFGRLPHGRIDMQDTSYDLLGDFRG"
BASE COUNT          460 a          997 c         1005 g          481 t
ORIGIN      
        1 tagctgagcg ctgcgtccat cacgtacatg tgctcgtgat cggtgatgaa gcgcacggcg
       61 tcggcgttgc ggggcaggat gggttcctcg gtcaggaccc gctggcggaa agccgggtcc
      121 ttgagcatgg cccgacgctc ggccaggggc cgttcgtgga tctcgcgata ggccggaaac
      181 tgccgcatgg ggttgatggt gccgtccagg gtcatgagga tggaggccgg ccgggcgccc
      241 acctggggcc tcaagttgaa gccttccgcg ccgagcttct cggcgagggt ccacagcggc
      301 ccgaagcccg cggtggtcag cggtgtgacc gtgcagccgg tggcgcgggt gatgcgctcg
      361 atccaggcca gttctgccgg gtcgtccagc tggtcggaga cgaactccat ggtgccgtag
      421 ccgaggcctt cgaaggcgcc ggcgatggcg cgcatctcgt cggcggacgc ccgggtgccg
      481 ggtaccagcc ggccggcctt gtccacgtgg ccgtagaagc gggaggtgga gaatcccagg
      541 gcgccggcct ccagggccgt ccgggtgaga tcgcgcatgg ccgtcatgtc ctcgggcgtg
      601 gcgtcgtcgt aacagcgctc gcccatgacg tagtggcgga tggcgacgtg aggcacctgg
      661 gcgccgacgt ccatgacgta gggggtgtcg atggcgtcca gatactcgga gaacgtctcc
      721 cagccccagg gaatgccgtc gtgcagcgcc gtgccgggga tgtcctccac gctctccatg
      781 agggccacga gctcaccctc cgtacccggc cgcaccggag caatgcccac gccgcagttg
      841 cccatgacga tggaggtcac cccgtgccag cagctcggcg tgagctgctt gtcccagcag
      901 acctggccgt cgtagtgggt gtgaatgtcc acgaagccgg gggtcaggag atccccgccc
      961 gcgtcgatga cccgggcggc gctcgcaggc gcagacccct tcgccgccac cgcgacgatg
     1021 gtgccgtcgt ccaccaccac gtcgccctct ctgcggggtg cgccggtgcc gtcgacgagg
     1081 gtggcgttgc ggatgatgag gtcgtgcatg ggctgtctcc cctacccttc gctggatccc
     1141 tgattgctca ggatactacc atgaggacca gtcaggccgg cgccgtcggt gtgccgaccc
     1201 tatcgaggag cgcgccatgt ccgaacccgt cctgaagatc ttctcctacc tgcccaatcc
     1261 ccgggtgtgg aaagcccaga tcgccgccga gctctgcggc gtggccgtgg acgtggtcgg
     1321 cgaccggccc gctgctctgc agaactggct gtgggacttc gaggcacggc cgctggagga
     1381 cgctgagcgc acggcgaaca gcccccacgc cagagaaagc cgccggggtt tctccggcct
     1441 tctcttcaag accgacgcct tcctcaccgc ccatcccttc ggcaccgtcc ccgccgcgtt
     1501 cgcgccggga ggcgagcccg gcatcttcga gtccaacagc atcctgcgcg cggtggcccg
     1561 ggcgggtgcc gccaaccggc agctgtacgg cggcgatggg taccaagcat cgcgcatcga
     1621 cggcttctta gacgccaacc tggttttcgc ccgggaagcc caggtctacc tgctgggcat
     1681 ggacgagccc caggctcacg tccacgagcg catggcggcc gcttaccagt tctatctcgc
     1741 cggcgtggag agcgcgctgg cgcgcagcgc cttcctggcc ggggacgagc tgagcattgc
     1801 cgacatctcc ttcgtctgcg acttcgccca gttcttgaga gaagggcact acgaggatgc
     1861 tctggaaaaa gccggccttt cgttgatcag cggcaacggc ccggaagact atccccgcag
     1921 cttccaccac ctgctcacgc tcagcgccga gccggcgttc gcgcagcaca tgggaaccta
     1981 tctggactgg tatcgaaggc gcgtcgcggc tgcctaaggg ccgttcgcgc cgctaccagt
     2041 tctcgccatg tggcctgagc tctgacagga acgtccacgc cgacgggtcc tggtgcgcca
     2101 ggtaccaggc gttctccgcg atggatgcag gctgaatgaa gaagccgtcg ggctggtcgg
     2161 caaagcgccg acgggtccag gcgaggtcga tcaccgcgtc gatgacgatg taggccacgt
     2221 gcacgtgctg gggaccaagt gagcgggcca tggactccgc gaggatccgc tgggccgcct
     2281 tggtgggggc aaacccggcg aagcccgcct tgccccgcaa agccgacgtg ttgcccgtga
     2341 cgatgatggc cccttccccc gcctcgatca tggcgggcgc caccgcccgg ccgaggtgaa
     2401 gcagacccat gacgttcacc tggaagttgg cctcgagctg agccggctcg atgctcagga
     2461 agtcgccgaa agcgccaccc acggcgttgt gaatggcgat cgaaggcggg ccgagctcgt
     2521 ccgcgacgcg ggcgagagtc gattcgatct gcgcgggatc cgccatgtcg cagggatagg
     2581 cgtgagcgtt cggcatctcg cctgccagcg cctccaggcg ctcggccgtg cgggctatca
     2641 tggccacccg gtagccgccc tcgtgaaacc gccgcaccat ggcggcaccg gtgcccggcc
     2701 ccaccccggt gacgacgcag accttctgct cagccacgga aatctcccag cagatcgtag
     2761 gacgtatcct gcatgtcgat gcgcccgtga ggcagccggc cgaagagccc gtgctgccac
     2821 tcggcggtgg acgcaccgtt gatgttccag tccatcacgt aaaggcggtg tccggccccg
     2881 tgaactcgat cagcacgttg tccacccgat gggtctgccg cctgacgccg agctcggcat
     2941 cga
//
LOCUS       DBNHOX010000141         1737 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_267924, whole genome shotgun sequence.
ACCESSION   DBNHOX010000141 DBNHOX010000000
VERSION     DBNHOX010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_267924"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..1358)
                     /locus_tag="AC16UZ_02990"
     CDS             complement(393..1358)
                     /locus_tag="AC16UZ_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245677.1"
                     /translation="MTREITAAAILAALFMLQGCSQKDEISGKEAAGNEPASPAHVVE
                     IRAVGMTFEGPSEIPSGWTTFKFVNASSMIHFALIDVPPEGITTELFTNTVGQYFQEA
                     MDGMNAGDEAAVNAAFAKFPAWIGDVGRHGGPGLLSPGLTGETTVYMKPGHYFIECYV
                     KSNGIFHTTSPGEGKLGMMLDLTVTEQQNNAPEPVANATLAIRNSGMEIVAGELRPGV
                     NTIRVDFVEQQALPSFVGNDVHLMRVESEESIGLADGWLDWRTKEGLEDPSPVVFLGG
                     LNDMPAGSHGYFTVDLQPGDYAFIAEVPNPAATGFVLRFTVATTS"
     gene            complement(1563..>1737)
                     /locus_tag="AC16UZ_02995"
     CDS             complement(1563..>1737)
                     /locus_tag="AC16UZ_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HIR6245678.1"
                     /translation="EKEDGTCVTYQIVGEDEADPGKGKISYVAPIAVLLIGAAEGDVV
                     AMVDGEAEVVGVE"
BASE COUNT          302 a          577 c          504 g          354 t
ORIGIN      
        1 gtacttcgtg ttttgttgtc ggacttggcc gtcggcgcga cctgcgtgct ggcgggactg
       61 cgcaggcagc acagcgacgg tgctgataag ggcgttcaat ccaccgcaca gccttgcctc
      121 tagttcactg tcccggtagg catcctgatt caattgccga ggcagtggga ttgggaatct
      181 accgatccag gcccgatttt cgcggcccgt ggcgggtagt acgctgcgag ccggacctgg
      241 ctatgggaca agaccccacc gccgctacca acctatcaag gaaccccggc cccgcgccgg
      301 ggttccttta ttccgccaat gtctgtacct ggctgcaggc gggcctctgc cggaggcggc
      361 ttctgcttcc gtcgtacccc ccagaaaatg acctagcttg tcgtcgctac cgtgaaccga
      421 aggacgaaac cggtcgccgc cggattcggc acctcggcga tgaacgcgta atcgccgggt
      481 tgcagatcca ccgtgaaata gccgtgcgat cccgccggca tgtcgttcag gccgcccagg
      541 aagactacag ggcttggatc ttccagccct tcctttgtgc gccagtcgag ccagccatcc
      601 gccaggccaa tgctttcttc gctctcaacc ctcatcaggt gcacgtcatt gccgacaaaa
      661 gacggcaggg cctgctgttc gacgaagtcg acgcggatcg tgttgactcc gggcctgagt
      721 tcaccggcaa caatctccat gccggagttc ctgatcgcca gcgtcgcatt ggccacgggc
      781 tcgggcgcgt tgttctgttg ttccgtcacg gtcaggtcga gcatcatgcc caacttgcct
      841 tcgccggggc tcgtcgtatg aaagatgccg ttcgacttta catagcactc gatgaagtag
      901 tgaccgggct tcatgtagac ggtcgtctca ccggtcaggc cgggtgacag caggcccggc
      961 ccgccgtggc gtccgacgtc accgatccag gcggggaact tggcaaaggc ggcattgacg
     1021 gcggcctcgt cgcccgcgtt catgccatcc attgcctcct ggaaatactg gcccaccgtg
     1081 ttggtgaaaa gctcggtggt aatgccctcc ggcggcacgt cgatgagcgc gaaatgaatc
     1141 atgctggagg cgttgacgaa cttgaatgtc gtccagccag atgggatttc cgaaggccct
     1201 tcgaaggtca tcccgacggc ccggatctcc acgacgtgag caggcgaggc cggttcgttg
     1261 ccggcagcct ccttgcccga gatctcgtct ttctggctgc agccctgcag catgaacaat
     1321 gcagccagga ttgctgcagc agtaatttcc ctagtcacgg ttccccccgt tctgtcgatg
     1381 tccgccggct tgcaatgaaa ccttaccacg atgcgccgtg caaggcccgg gaccaccgtc
     1441 gacaagtcag ccgcgattgc gagcgtcggg ccaaaggtcc ggtcttggcc gttagcagac
     1501 gttcacaggc cccggccccg cgccggtggc tggtccgacg agatccggtt tggatctaac
     1561 gctcactcga cacccactac ctccgcctcc ccgtccacca tcgctacgac atcgccctcc
     1621 gccgccccga tcagcaacac cgcaatcggc gcgacgtagg aaatcttccc ctttcccggg
     1681 tccgcttcgt cctcgccgac gatctggtag gtgacgcacg ttccgtcctc tttctcg
//
LOCUS       DBNHOX010000142         2806 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1039803, whole genome shotgun sequence.
ACCESSION   DBNHOX010000142 DBNHOX010000000
VERSION     DBNHOX010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1039803"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="AC16UZ_03000"
     CDS             <1..205
                     /locus_tag="AC16UZ_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245679.1"
                     /translation="WEVGYRLGQASEFSLLVSYVALGAGLLGKEAAHVIQGATVLTLL
                     LSSYAVIFRYPSPIAISARLRRD"
     gene            complement(209..1201)
                     /locus_tag="AC16UZ_03005"
     CDS             complement(209..1201)
                     /locus_tag="AC16UZ_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HIR6245680.1"
                     /translation="MTGFRALLVEKTETGFQRSVVTRRVEDLPPGDLLIDVRYSSLNF
                     KDALSATGNPGVSRNFPHTPGIDAAGVVVESSSEGFAPGDAVVAIGFDLGMNTPGGFG
                     QRIRIPAGWALPLPEGLDLRESMRLGTAGFTAALCVQKLEEAGMTPDGGPVLVTGATG
                     GVGSVAVMLLAKLGYRVTAVTGKPDQHGFLESIGAAEIVSREEARSGSDRPLLPETWG
                     GVVDTVGGETLFNSIKRLRYGASLAACGLVESPQFAATVLPFILRNVNLLGVDSVELP
                     LAEKARVWDKLAGPWKLEGLESLEETLSLDTVSDAIDRILGGAMVGRGVVDLYA"
     gene            complement(1198..2238)
                     /gene="sohB"
                     /locus_tag="AC16UZ_03010"
     CDS             complement(1198..2238)
                     /gene="sohB"
                     /locus_tag="AC16UZ_03010"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019470684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HIR6245681.1"
                     /translation="MEYLLDYLLFLAQAVTVVVAVLVVLSGVASVSARFQGAERGGHL
                     EVKKLNDRLEELKSGLQQALLDSSTYKKQRKRDAKAAKQRAKVERAEAAKADSEHRKR
                     VFVLDFHGDLQASAVHDLTTELTAVLTLADDRDEIVVKVESPGGLVHGYGLAASQLDR
                     VREHGVPLVVAVDKVAASGGYLMAAVADRVVAAPFAVVGSIGVVAQVPNVHRLLKKHD
                     VDVEVLTAGKFKRTLTVLGENTEEGRKKFIEDLEDTHALFQEFVAARRPQLDMATVAT
                     GETWHGQRAMDLKLVDELCTSDEYLVRSCETADVFSVRWVEHKKPLERIMAKVETGVQ
                     NIVDQLFRRPGP"
     gene            2370..2702
                     /locus_tag="AC16UZ_03015"
     CDS             2370..2702
                     /locus_tag="AC16UZ_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HIR6245682.1"
                     /translation="MPHDDLTRRLQSAFHPPAASHMDATFALDLGQLRISFRVRQGRL
                     DFVEGVPADATFYFDTPDEALQLLTGDADVFEAFMEGRFRADGYLMWTFALLSMFRGD
                     GAEPTPTG"
BASE COUNT          491 a          964 c          863 g          488 t
ORIGIN      
        1 gtgggaggtc ggctatcgcc tgggacaggc ctcggagttc tccctgctgg tgagctacgt
       61 cgcgcttggc gccgggctgc tggggaagga ggccgcccac gtcatccagg gcgcgaccgt
      121 actcaccctg ctgctgtcca gctatgcggt gatcttcaga tatccgagcc cgattgcaat
      181 atcggcccgg ctgcgcaggg actgagtttc aggcgtacag gtccaccacg ccacgtccca
      241 ccatggcacc gccgagtatc cggtcgatgg catccgagac ggtatccaga gagagcgtct
      301 cctcgaggga ctctaaacct tcgagcttcc aggggcccgc cagcttgtcc cagaccctcg
      361 ctttctccgc caggggcagc tcaaccgaat ccacgccgag caaattgacg ttgcgcagga
      421 tgaagggcag cacggtggcc gcgaactgcg gcgactccac gagaccgcaa gcggccaggc
      481 tcgccccgta acgcaggcgc ttgatcgagt tgaagagcgt ctcgccgcca acggtgtcca
      541 ccaccccacc ccaggtttcc ggcagcaacg gcctgtccga cccggagcgc gcttcttccc
      601 gggacacgat ctctgcagcg ccgatggact cgagaaagcc gtgctgatcg ggtttgcccg
      661 tcaccgcggt gacccgatag ccgagctttg ccagcagcat gacggcgacg gatcccacgc
      721 caccggtggc gccggtcacc agcaccggac cgccatccgg cgtcatgccc gcctcttcga
      781 gcttctgcac gcacagggcg gccgtgaaac cggccgttcc gaggcgcatg gattcgcgaa
      841 gatccaatcc ctccggtagc ggcagggccc atcccgccgg aatccggatg cgctggccga
      901 aaccgcccgg cgtgttcatg ccgaggtcga aaccgatggc gaccacggcg tcgcccgggg
      961 cgaagccctc gctggacgat tccacgacca cgcccgcagc gtcgatgccc ggggtgtgcg
     1021 gaaaattacg gctcacgccc ggattgccgg tggcggaaag cgcatctttg aagttgagcg
     1081 aggagtagcg aacgtcgatc agcagatcgc cgggcggcaa atcctccacg cggcgcgtga
     1141 cgaccgagcg ctggaaaccg gtttcggtct tctccaccag cagggcacgg aaacccgtca
     1201 tggtccgggc ctcctgaaca gctgatcgac gatgttctga acgccggtct ccactttggc
     1261 catgattcgt tccaacggct tcttgtgctc cacccagcgc acgctgaaca catccgccgt
     1321 ctcgcacgag cgcaccaggt actcgtcgct ggtgcaaagc tcgtcaacca gcttgaggtc
     1381 catggcccgc tgcccgtgcc aggtctctcc ggtggccacg gtggccatgt ccagctgcgg
     1441 acgccgcgcg gccacgaatt cctgaaacag cgcgtgggtg tcctcgaggt cctcgatgaa
     1501 cttcttccgg ccctcttccg tattctcacc aagcacggtc aacgtgcgct tgaacttgcc
     1561 ggccgtgagc acctccacgt ccacgtcgtg ctttttcaga agccgatgga cgttgggtac
     1621 ctgggccacc acgccgatgg atcccacgac cgcaaacggg gccgcgacca ccctgtcagc
     1681 cacggcagcc atcagataac caccgctcgc ggcgaccttg tccaccgcga cgacgagcgg
     1741 caccccgtgc tcacgcaccc ggtcgagctg agaagccgcc aggccgtatc cgtgcaccag
     1801 cccgccgggg ctttccacct tgaccacgat ctcgtcacgg tcatcggcga gcgtgagcac
     1861 agcggtcagc tccgtcgtga gatcatggac ggcgctcgcc tggagatcgc cgtggaagtc
     1921 gaggacgaag acccgcttgc gatgctcgct atcggccttg gcagcctcgg ccctctcgac
     1981 tttggctcgc tgcttggcgg ccttggcatc ccgcttgcgc tgctttttgt aggtcgagct
     2041 gtcgagcaga gcctgctgca gccccgactt cagctcctcg agccggtcat tgagcttctt
     2101 gacctcgaga tgaccgcccc gctccgcacc ttggaacctg gcgctcacgg aagcgacgcc
     2161 ggaaagcacg acgagaacgg ccacgacgac cgtgacggcc tgggccagaa agagcagata
     2221 gtcgagcaga tactccatgc caccccggaa tttttacgca aagcaagggc gcgctaatgt
     2281 actggctgcg tcaacgggcg tccaccgccg cgcctgcagc cacatgcgca cggcttgatc
     2341 tcatccgcac atgcgtgtct gattcaacga tgccccacga cgacctgacc cggcgactgc
     2401 agagcgcgtt tcatccgccg gcggcttccc acatggacgc cacgtttgcc ctcgatctgg
     2461 gccagctccg cattagtttt cgcgtccgtc agggccggct cgatttcgtg gagggcgtcc
     2521 cggcagatgc gaccttttac ttcgacacgc cggatgaggc gctgcagctg ctcacgggcg
     2581 atgcggacgt gttcgaggcc ttcatggagg gccgctttcg cgcggacggc tacctgatgt
     2641 ggacttttgc cctgctgagc atgtttcgcg gggacggggc agagcccaca ccaaccggct
     2701 gaaggccgca tcagccggag ctgcggcgcc cgacttcctc gatgaagtca tcgatgagcc
     2761 atcgggagat ggcggtagca gggggcacgc tgggcagctc gtcgtg
//
LOCUS       DBNHOX010000143         2166 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1360812, whole genome shotgun sequence.
ACCESSION   DBNHOX010000143 DBNHOX010000000
VERSION     DBNHOX010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1360812"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1515
                     /locus_tag="AC16UZ_03020"
     CDS             <1..1515
                     /locus_tag="AC16UZ_03020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6245683.1"
                     /translation="EISDLKFIASEIGGGFGGKTVVYLEPLAVLLARKSRRPVKMMMT
                     REEVFRATGPTSGTHCRIKVGAKRDGTITSATAWLAYEAGAFAGAPMGPGAMSIFAPY
                     DLENFKIEAFDVLLNKPKVAAYRAPGAPQSMHAMEVALDDLALELGMDPIDLRMKNAA
                     GEGTQAPYGPKFPQIGFKECLEAAKAHPNYTKPLPEGAGRGVAAGFWFNVGMQSSAEV
                     HVNEDGTVTVIEGNPDIGGSRASMCMMASETLGVPYEKIRALVGDTESTGFSNVTGGS
                     RTTFATGMAVVQACEDIITQCKARAAATWGMDADQVDWKDGQAVPAPGVNADVTPLSL
                     ADIAAKAGRTGGPLLGRASLNAQGAGASFSVNICDLQVDPETGKVDVLGFTAIQDAGK
                     AIHPSYVEGQMQGGAAQGIGWALNEEYIFDENGVMENAGFLDYRIPVASDLPMIDCHI
                     VEVPNPSHPFGVRGVGETPIVAPLAATANAFRAATGVRVNDLPLSPPRVLSALD"
     gene            1569..1811
                     /locus_tag="AC16UZ_03025"
     CDS             1569..1811
                     /locus_tag="AC16UZ_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin family protein"
                     /protein_id="HIR6245684.1"
                     /translation="MARVVFPEHLLQHTGGEREIEVPAADFRELLINLERRWPEAGAV
                     LEKCAVAIDGQIYQDAFLEPLEPDSEVFFMQRIEGG"
     gene            complement(1821..>2166)
                     /locus_tag="AC16UZ_03030"
     CDS             complement(1821..>2166)
                     /locus_tag="AC16UZ_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR6245685.1"
                     /translation="SCKVSYQVACITNNVKSGQGPAMASNRARAAAMEGVMALFDLVV
                     ESSIEGIRKPDPRIYELTCERLGVRPEQAVFLDDLGVNLKPARALGMHTIKVVEESQA
                     LEELGSLTGLAL"
BASE COUNT          400 a          664 c          726 g          376 t
ORIGIN      
        1 gaaatctccg atctcaagtt cattgcctcg gagatcggcg gcggctttgg cgggaagacg
       61 gtggtctacc tcgagccgct ggctgtgctg cttgccagga agtcccgtcg tccggtgaag
      121 atgatgatga cccgggagga agtctttcgg gccaccggac ccacctcggg cacccattgc
      181 cggatcaagg tgggtgccaa gcgcgacggg accatcacct cggcaacagc ctggctggcg
      241 tacgaagccg gtgcctttgc cggggctccc atgggcccag gtgccatgtc gatcttcgcg
      301 ccctacgacc tggagaactt caaaatagaa gcctttgacg tgctcctcaa caaacccaag
      361 gtggctgcct atcgggcgcc gggagcgccg cagtccatgc acgccatgga agtcgcgctc
      421 gacgatctgg cgctcgagct cggaatggat cccatcgatc tgaggatgaa gaacgcggcg
      481 ggcgagggga cgcaggctcc gtacgggccc aagtttcccc agatcggttt caaagaatgc
      541 ctggaggccg caaaagccca tccgaactac acgaagccgt tgcctgaggg tgctggcagg
      601 ggtgttgcgg ccggcttctg gttcaacgtc ggcatgcagt cgagcgccga ggtccacgtc
      661 aacgaggacg gcaccgtgac cgtcatcgag ggcaatcccg acatcggcgg ttccagggcg
      721 tccatgtgca tgatggcgtc cgaaacgctc ggtgttccct acgagaagat cagggctctg
      781 gtgggcgaca ccgaaagcac cggattctca aacgtgacgg gcggaagccg gacgacgttt
      841 gcgacgggaa tggccgtggt ccaggcctgc gaggacatca tcacccagtg caaagcccga
      901 gcggccgcaa cctggggaat ggacgccgac caggtggatt ggaaagacgg tcaggcggtg
      961 ccggcacccg gtgtgaatgc cgacgtcacg cccttgagcc tcgcggacat cgcggccaaa
     1021 gccggcagga ccggcgggcc gctgctggga cgcgcatcgc tcaatgccca gggcgccggc
     1081 gcatcgttct cggtgaacat ctgcgatctg caggtggacc cagaaactgg caaagtcgac
     1141 gtcttgggct tcaccgccat ccaggatgcg ggcaaggcca tccatccttc gtacgtcgaa
     1201 ggccagatgc agggtggggc tgcccagggt atcggctggg ctctcaatga ggaatacatc
     1261 ttcgacgaga acggggtcat ggagaacgcc gggttcctgg actaccggat tccggtggca
     1321 tcagatctgc ccatgatcga ctgtcacatc gtcgaggtgc ccaacccgag ccatccgttt
     1381 ggcgtgcggg gggtgggcga gacgcccatc gtggcgcctc tggcggccac ggcgaatgcg
     1441 ttcagagcgg caacgggcgt tcgtgtgaac gacctgccgc tgtcgccgcc gcgcgtgctc
     1501 tcagctctgg attgacggga atcaaccggg gacggaagtc cccggttctc cccggagcgc
     1561 cagcacccat ggccagagtc gtgtttcccg agcacctgct tcagcacacg ggcggagagc
     1621 gggagatcga ggtgcccgcg gccgattttc gcgagctcct gatcaacctc gaaaggcgtt
     1681 ggcccgaggc gggggcggtg ctggagaagt gtgcggtggc catcgatggc cagatctatc
     1741 aggacgcgtt tctggaaccc ctcgagccag acagcgaggt cttcttcatg cagcgaatcg
     1801 agggcggctg agcgtccctc tcagagcgca agcccagtga gggagccgag ttcttcaagc
     1861 gcctgacttt cctcgaccac tttgatggtg tgcatgccca gggcacgcgc gggtttgagg
     1921 ttgacgccga ggtcgtcgag gaacacggcc tgctcgggtc ggacgccgag ccgctcgcac
     1981 gtgagctcat aaatccgcgg gtcgggctta cggatcccct cgatgctgga ctccaccacg
     2041 aggtcgaaca gcgccatgac cccttccatg gccgcggctc gcgcccggtt gcttgccatg
     2101 gccggtccct ggccggactt gacgttgttg gtgatgcacg ccacctgata cgacaccttg
     2161 cagctg
//
LOCUS       DBNHOX010000144         5508 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_333158, whole genome shotgun sequence.
ACCESSION   DBNHOX010000144 DBNHOX010000000
VERSION     DBNHOX010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_333158"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /gene="ssb"
                     /locus_tag="AC16UZ_03035"
     CDS             complement(<1..362)
                     /gene="ssb"
                     /locus_tag="AC16UZ_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR6245686.1"
                     /translation="MARGINKVILIGNLGRDPETRYSQGGNAVTSFSVATTENWRDRQ
                     SGEQQERTEWHNVVCFARLAEIAGEYLRKGSKVYIEGSLRTSSWEQDGQKRYRTEVMA
                     RDLQMLDSRGGGQGGQGGG"
     gene            572..3397
                     /gene="uvrA"
                     /locus_tag="AC16UZ_03040"
     CDS             572..3397
                     /gene="uvrA"
                     /locus_tag="AC16UZ_03040"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR6245687.1"
                     /translation="MDSIAVRGARTHNLKNVNLDIPRDRLVVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSMMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIYDYLRLLYARVGEPRCPNHDLPLDAQTVSQMVDQVLGLPEDKRVMVLAPVVTERKG
                     EHLHVFQELMANGFIRARIDGIVVDLDHPPNLEKNKKHTVEAVIDRLRIRADAQQRLA
                     ESFETALDLAEGIAKVASMDDADEPELVFSARFACPACGYSISELEPRMFSFNNPAGA
                     CQACDGLGVKQFFDPGLVVQDEELTLAEGAIRGWDRRNVYYFHMLSSLAAHYGFDVET
                     PFRKLPKKHRDAILYGSGRERIDFSYANDRGDVIHRRHRFEGVIPNMERRYHETDSSL
                     VRENLQKYLRVRSCPDCGGTRLREASRNVFIDGVSLPEITSGSVEGSLAYFDELKLSG
                     RRGEIADKILKEIRARLRFLVDVGLNYLTLDRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYILDEPSIGLHQRDNARLLKTLEHLRDLGNTVLVVEHDEEAIRSADHIIDIGPGAGV
                     HGGCVVAAGSLEKILKSRRSITGQYLSGRKKIPVPEKRTAFVEEETIRIIGATGNNLQ
                     SVSVEIPCGLLTCVTGVSGSGKSTLINHTLYPVAATRLNKATTLTPAPVEEVLGLNHV
                     DKVVDIDQSPIGRTPRSNPATYTGLFTPIRELFAQTQEARTRGYKPGRFSFNVKGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDVCKGKRYNRETLDVRYKGRNIHEVLEMTVEEARE
                     FFDAVPMLARKLQTLVDVGLSYIALGQSATTLSGGEAQRVKLSRELSKRDTGKTLYIL
                     DEPTTGLHFHDIAQLLDVLHRLRDQGNTVVVIEHNLDVIKTADWVVDLGPEGGSGGGR
                     VVAAGTPEHLARKRGSHTGRFLKKMLG"
     gene            complement(3415..3861)
                     /gene="rplQ"
                     /locus_tag="AC16UZ_03045"
     CDS             complement(3415..3861)
                     /gene="rplQ"
                     /locus_tag="AC16UZ_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR6245688.1"
                     /translation="MRHRKSGRHLNRTSSHRQAMFRNMAASLIEHEVIKTTLPKAKEL
                     RRVAEPLITLAKEDSVANRRLAFARTRSDAAVGKLFVELGPRYKDRPGGYTRILKCGN
                     RAGDAAPMAFIELVDRPVVDEFDEDEFDAADEQADEAEELEAEEKG"
     gene            complement(3952..4947)
                     /locus_tag="AC16UZ_03050"
     CDS             complement(3952..4947)
                     /locus_tag="AC16UZ_03050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR6245689.1"
                     /translation="MAQSVTEFLTPRHIDIEASSPTRAKVTLEPLERGFGHTLGNTLR
                     RILLSSMPGCAITEVQIDGVLHEYSTLEGVQEDVINILLNLKGIAVILHSQDEAVITL
                     NKDGAGQVTAGDFQLTQDVEIANPDHLICSLNDNGAIRLEARVTRGRGYVPADSREEE
                     EDSRPIGVLSLDATFSPIRRVAYGVESARVEQRTDLDKLVLDLETNGTIEPEEAIRRA
                     ATILQQQLAVFVDLESEGEPVAEEKEDEIDPILIRPVDDLELTVRSANCLKAENIYYI
                     GDLIQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASLRGSDKVA"
     gene            complement(4995..>5508)
                     /gene="rpsD"
                     /locus_tag="AC16UZ_03055"
     CDS             complement(4995..>5508)
                     /gene="rpsD"
                     /locus_tag="AC16UZ_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR6245690.1"
                     /translation="PPGQHGARRGRLSDYAIQLREKQKVRRLYGVLEKQFRNYYKKAE
                     RQKGATGENLLRLLECRLDNVVYRMGFGATRAESRQLVSHKAITVNGSLVNIPSYQVA
                     ADDVVGVYEKARGQLRIQSAMQLASQRAPVEWVDVDPTKLEGVYRRPPDREELPPEIN
                     ENLIVELYSK"
BASE COUNT          972 a         1803 c         1677 g         1056 t
ORIGIN      
        1 ccgccgccct ggccgccctg accgcccccg cggctgtcga gcatctgcag gtccctcgcc
       61 atgacctcag tccggtagcg tttctgaccg tcctgctccc agctgctggt acgaaggctg
      121 ccctcgatgt aaaccttcga gcccttgcgc agatactcac ccgcgatctc ggcaaggcgc
      181 gcgaagcaca ccacgttgtg ccactcagtg cgttcctgct gctcgcccga ctggcggtcc
      241 cgccagtttt ccgtggtggc gacgctgaag ctggtgaccg cattgccgcc ctgggagtag
      301 cgcgtctcag gatccctgcc cagattgccg atgagaatga ctttgttgat accccgcgcc
      361 atagcctttt tcccgttgcc tcttacccgt tcaaatgccc agaaaactcc aggcccgcag
      421 tctagcacgg cgccgtgaaa agtccgtaag cgatggtcca gcttgcccct gagtagggat
      481 cgatatatgt tgccgggttt ccccacttcc ggggtcaatc agcgcaagct agggtcggcg
      541 agccgcgctg gcagggcata aggcacgcgc attggacagc atcgcggttc gcggggcacg
      601 cacccacaat cttaaaaacg tcaatctgga cataccccgg gatcggctcg tggtgatcac
      661 ggggctatcg ggatccggaa agtcctcgct tgccttcgac accctctatg cggaggggca
      721 gcgccgctac gtggagtccc tctcggccta cgcccgccag ttcctgtcga tgatggagaa
      781 gccggacgtc gaccatatcg agggcctgtc cccggccatc tccatcgagc agaagtccac
      841 ctcgcacaat ccccgctcca ccgtgggtac gatcaccgag atctatgact atctgcggct
      901 gctgtacgcg agggtcggcg agccgcgttg ccccaaccac gacctgcccc ttgacgccca
      961 gacggtcagc cagatggtgg accaggttct cggcctgccg gaggacaagc gggtaatggt
     1021 cctcgcgccc gtggtcaccg aacgtaaggg cgagcacctg catgtctttc aggaactcat
     1081 ggcaaacggc ttcatcagag cccggatcga cggcatcgtg gtcgatctcg accacccgcc
     1141 aaacctggag aagaacaaaa agcacaccgt ggaagccgtc atcgaccggc tgcgtatccg
     1201 ggcggatgcg cagcagcggc tcgctgaatc cttcgaaacc gctctagatc tcgccgaggg
     1261 gatcgcgaag gtggcgtcca tggacgatgc cgacgagccg gaactcgttt tctcggcaag
     1321 gttcgcatgt cccgcctgcg ggtacagcat atccgagctc gagccccgca tgttctcctt
     1381 caacaacccc gccggcgcct gccaggcttg cgacggcctt ggggtgaagc agtttttcga
     1441 tcccggcctg gtggtccagg acgaggagct gacgctggcc gaaggcgcca tacgcggctg
     1501 ggaccggcgc aacgtttact acttccacat gctctcctct cttgcagccc actacggctt
     1561 cgacgtggag acgcctttcc gaaagctgcc caaaaagcat cgggacgcaa tcctctacgg
     1621 cagcggcaga gagcggatcg atttcagcta cgccaacgac cgcggcgacg tgatccatcg
     1681 ccggcaccgg tttgagggcg tgataccgaa catggagcgc cgttatcacg agaccgactc
     1741 cagcctggtc cgagagaacc tgcagaagta ccttcgggtc aggagctgcc ccgactgcgg
     1801 cggcacgcgg ctgcgcgagg cgtcgcgcaa cgtcttcatc gacggcgtca gcctgccgga
     1861 gataacgtcc gggtccgtgg agggctccct ggcctacttc gacgagctca agctgtcggg
     1921 gcgacgggga gagatcgccg acaagatcct caaggagatt cgcgcccgtc tgcggtttct
     1981 ggtcgacgtt gggctcaact atctcaccct ggaccgcagc gccgagacgc tttcgggggg
     2041 ggaagcccag cgcatccgcc tggcaagcca gatcggcgcc ggcctggtcg gcgtcatgta
     2101 cattctcgac gagccttcca tcggcctgca ccagcgggac aacgcccggc tgctcaagac
     2161 tctggagcac cttcgcgatc tgggaaacac ggtgctggtg gtagagcacg acgaggaggc
     2221 gatccgctcc gcagaccaca tcatcgacat cggaccaggg gccggcgtgc acggcggctg
     2281 cgtggttgcc gccggcagtc tcgagaagat cctgaaatcc aggcgctcca tcaccggcca
     2341 gtatctctcc ggtaggaaga agattcccgt gcctgaaaag cggacggcgt tcgtcgagga
     2401 ggagaccatc cgcatcatcg gcgccaccgg caacaatctt cagtccgtgt cggtcgagat
     2461 accgtgcggc ctgctgacct gcgtaaccgg cgtttccggc tcgggcaagt cgacgctgat
     2521 caaccacact ctctaccccg tggcagctac cagactcaac aaagcaacca cgctgacgcc
     2581 ggcacccgtg gaggaggttc tgggtctgaa tcatgtcgac aaggtggtcg acatcgacca
     2641 gagccccatc ggccgcacgc cgcgctccaa tccggcaacg tatacgggtc tgttcacgcc
     2701 gatccgtgag ctcttcgctc agacccagga ggccaggacc cggggttaca agcccggccg
     2761 cttcagcttc aacgtcaagg gcgggcgctg cgaggcgtgt cagggtgacg gcgtcatcaa
     2821 ggtggagatg cacttccttc ccgacatata cgtgccatgc gacgtctgca aaggcaaacg
     2881 ctacaaccgg gagacgctcg acgtccgcta caaaggcagg aacatccacg aggtcctgga
     2941 gatgaccgtg gaggaagccc gggaattttt cgacgcggtt cccatgcttg cacgcaagct
     3001 tcagaccctg gtggacgtgg ggctgtcata catcgccctc ggacagagcg cgacgacgct
     3061 ttcaggcggc gaggcgcaac gcgtcaagct gtccagggag ctttccaagc gggacaccgg
     3121 caagaccctg tacatcctgg acgagcccac tacgggcctt cacttccacg acatcgccca
     3181 gctgctggac gtgctgcacc gtctgcgcga tcagggaaat acggtggtcg tgatcgagca
     3241 caacctggac gtcatcaaga cggccgactg ggtggtggac ctggggcccg agggcggctc
     3301 tggcggcggc cgggtggtgg ccgcagggac cccggagcat ctcgccagaa agcgtggctc
     3361 tcacaccggg cgttttctca agaagatgct ggggtaggtg ccttcgcgcc aggctcagcc
     3421 cttctcctcg gcttccagct cttctgcttc gtccgcctgc tcgtccgccg catcgaactc
     3481 gtcttcgtcg aactcgtcga ccacgggacg atccaccagc tcgatgaacg ccatgggcgc
     3541 cgcatcgccc gcccgattgc cgcatttcag gatgcgggtg tagccgcccg ggcgatcttt
     3601 gtagcgcggt cccaactcta cgaacagctt acccaccgcc gcgtcgctgc gcgtgcgcgc
     3661 gaatgccagg cgccggttgg cgacgctgtc ttccttcgcg agggtgatga gaggctcggc
     3721 gacgcgccgg agctctttcg ctttggggag cgtcgtcttg atgacctcgt gctcaatgag
     3781 cgacgcggcc atgttacgga acatagcctg ccggtgtgag ctcgtgcgat tgagatgtcg
     3841 tccactcttg cgatgtcgca tgactgcttc ctaagttact ccgcgccttt gccgcgacga
     3901 cggcccgaga tcaccacctc cggccttcct cgacctcttc aggcgacctc ttcaggcgac
     3961 cttgtcgctg cctctgagac tggccggagg ccagttctcc aggcgcatgc caagggacag
     4021 accgcgagac gccaggacgt ccttgatctc ggtcagtgat ttcttaccga gattgggggt
     4081 cttgagcagc tcaacctcgg tgcgctgaat gaggtcgccg atgtagtaga tgttttccgc
     4141 cttgagacag ttggccgagc gcaccgtgag ctcgagatcg tccacgggtc ggatgaggat
     4201 cgggtcgatc tcgtcttcct tctcctccgc gacaggctcg ccctcgctct ccaggtccac
     4261 gaacaccgcc agctgctgct ggagaatggt cgccgcccgg cggatcgcct cctcgggctc
     4321 tatggtcccg ttggtctcca gatccagcac gagcttgtcc aggtcggttc gctgctcgac
     4381 ccgggcgctc tcgacgccgt aggcgacccg acgaatcggg ctgaaggtag cgtcgaggct
     4441 gagcacaccg atcggccggg agtcctcttc ctcttcacgg ctgtcggccg gcacgtagcc
     4501 ccgaccgcgc gtgacacggg cttccaggcg aatggcgccg ttgtcgttta gagagcagat
     4561 caagtgatcc ggattcgcga tctccacgtc ctgagtcagc tggaagtcac cggccgtcac
     4621 ctggccagcg ccgtccttgt tcagggtgat taccgcctcg tcctggctgt ggagaatcac
     4681 agcgatccct ttcaggttga gcaggatgtt gatgacgtct tcctgcacgc cttcgagggt
     4741 gctgtactcg tgcagcacgc cgtcgatctg cacctcggtg atcgcgcagc ccggcatgga
     4801 tgacagcaga atgcgccgca gcgtgttgcc cagcgtgtga ccgaagcccc gctcgagagg
     4861 ctccagcgtc accttcgccc gggtcgggct gctggcctct atgtcgatgt ggcgtggggt
     4921 cagaaattcg gttaccgact gtgccatatg tttcgtctcc tgatatctgg agtgttcagt
     4981 tactcttcgg gcccttactt ggagtagagc tccacgatga ggttctcgtt gatctccggc
     5041 ggcagctctt cccgatccgg cgggcgcctg tacacgccct cgagcttggt cgggtccacg
     5101 tccacccatt ccaccggcgc ccgctggctg gccagctgca tagccgactg gatgcgcagc
     5161 tgcccgcgtg ccttttcata cacgccgacc acgtcatcgg cggccacctg gtaggagggg
     5221 atgttgacca gcgaaccgtt caccgtgatg gccttgtgcg agaccagctg ccgtgattcc
     5281 gctctggtcg ccccgaagcc catgcgatac accacgttgt ccaggcggca ttccaaaagc
     5341 ctgagcaggt tttcgccggt ggcgcccttc tgtcgttccg ccttcttgta gtagttgcgg
     5401 aactgcttct cgaggacgcc gtaaaggcgt ctgactttct gcttttcacg cagctggatc
     5461 gcgtagtcgg acaacctgcc gcgccgagca ccatgctgac cgggaggc
//
LOCUS       DBNHOX010000145        14531 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_397285, whole genome shotgun sequence.
ACCESSION   DBNHOX010000145 DBNHOX010000000
VERSION     DBNHOX010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 14531)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14531)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14531
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_397285"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="AC16UZ_03060"
     CDS             complement(<1..315)
                     /locus_tag="AC16UZ_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR6245691.1"
                     /translation="MDLGISERVKPLIDDITQYIHDYVVPNERAYAEQVDAGGRWTET
                     EAMEEMKEKAKERGLWNFFLPDSERGSGLNNLDYAHVAEVLGRYPLSSEAMNCAAPDT
                     GNM"
     gene            440..1144
                     /locus_tag="AC16UZ_03065"
     CDS             440..1144
                     /locus_tag="AC16UZ_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6245692.1"
                     /translation="MTAPGDATVRLLNDLFGGGVKSAVVLMRHSAREFAPGRHDLDNP
                     LTEEGRDLARRLGERLPEDLTVRAYASPPERCMETAELVLAGYASTGGRVTRHRPVEA
                     LGVFYALDQMKMWQVMQSAGGMVPFLEAWFSGDVPQDAVMPPRLAAALVVRLMTDKLS
                     AMDGARRLDVCVSHDMTLHLVRDRLLGQSVRDVDVDFLDGLVAFEREGARWLASHHGE
                     PVKLDAQLLSLGGLWD"
     gene            complement(1221..2090)
                     /locus_tag="AC16UZ_03070"
     CDS             complement(1221..2090)
                     /locus_tag="AC16UZ_03070"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR6245693.1"
                     /translation="MAYPRPELLEEPAALAARLDDADLRIVDATVFLVPAERGYRAES
                     GRERYEQGHIPGAAFMDLIDAFSDTTTGLGFSLPDPGVIADALGRLGISDDSEVVVYS
                     TGHLMWATRAWWLLRYVGHQRASVLNGGFDAWRDLGLPFETGTARRAAVTYRPRPDAS
                     LFTDRDGMCRAMDEPGVCTVNALSPEVYAGTGTTNYGRPGHIPGSVNVPYEELLAGGR
                     FKPAGALAEALESTGLLDADRVVTYCGGGIAATLDGFACLLLGHDNVAVYDGSMSEWA
                     RDPSLPLNVGEAP"
     gene            complement(2167..3429)
                     /locus_tag="AC16UZ_03075"
     CDS             complement(2167..3429)
                     /locus_tag="AC16UZ_03075"
                     /inference="COORDINATES: protein motif:HMM:NF028178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR6245694.1"
                     /translation="MSKREPGLAAFAALMLGFTGTNLAGCSPSVTEVDLIMPELPVEQ
                     IIARKIADLVDEDSGLKINLIPLPDDSLSVLDALEQGYGDIAFAPNTNEHRDTITTIM
                     PIYPSVLHVGSIADRADGSLEELLDGARVFAGPPGSIPRQIGERIVSGLQLEKGLSFT
                     SDQDAAPDVIILFAPIDRRRVMSNPSLESLKLFSFGDPGDTRPGSIVDRTVLLNPTLR
                     PYVIPAGTYGSLTPDPVVTFAVDTLLVARESLDETLVFDLFEEFLRLRPALFSKRPEL
                     FQPIDDDIARAHWTFSLHPGALDYLQRDEPSLIERYSGVAEVLVTLLVGMVSGAFAIL
                     KIYQVRRKNRIDAFYTQVIRTRDSVAEGAGSEERAAALAQLRSLRERAYEMLVEEQLA
                     ADTSFLIFVLLTNDAIAQIDSPENGPSV"
     gene            complement(3422..4282)
                     /locus_tag="AC16UZ_03080"
     CDS             complement(3422..4282)
                     /locus_tag="AC16UZ_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245695.1"
                     /translation="MIDAAVRRFDAFFLTEAQETFEEDRNRLRLRLDTDYIEHHGWEF
                     SPRVKVNFHLPGLGERVRLVVNDAGGDEEDEGVELEDDENDVALRWVARKGKKTGISV
                     DGGVSFRGGEVDPFVRVNTSYKYRISENWRGQATNRLYYLARNGWRDDLRIYFNRPIG
                     ERFLFRSRSRLEYFEDKDSNPQLEQKFSLFQNISDRSAIAYEAIWRQDTAEDSIYDDE
                     EILIGPRDKYQQVKLRIRYRRNFWRPWMFVEFWPVVGWVEERDWDTVLGARMRLEINF
                     GWHAGSKLDE"
     gene            complement(4474..4899)
                     /locus_tag="AC16UZ_03085"
     CDS             complement(4474..4899)
                     /locus_tag="AC16UZ_03085"
                     /inference="COORDINATES: protein motif:HMM:NF017913.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HIR6245696.1"
                     /translation="MMQLIANIERWVVLGLLVLMLLVVVASSIELAIIIVQAALENHF
                     SEEPAYLLLDINELLGIFGFFLLVLIGLELLASVKMYLEDDIVHVELVMLIALIAVSR
                     KIIVIDYAKVDGVTVLGIAALVLVLSVGYYLMKRSHEPS"
     gene            5137..5796
                     /locus_tag="AC16UZ_03090"
     CDS             5137..5796
                     /locus_tag="AC16UZ_03090"
                     /inference="COORDINATES: protein motif:HMM:NF017534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6245697.1"
                     /translation="MQIRFWRDRRPWRPVSMHQSAPNPCLERHWGGLRLEHGSTVFVP
                     MCGKSDDLRWLLGRGHQGLGIELADLAIEAYFEEAGEPYEREDSDRLVRYRGPRTDIL
                     CGDFFALTAAELSAARAVLDRGALIALPPKMRARYVDHLLRVIPDRTDILLVTLEYDQ
                     NLVAGPPFSVLPEEVVSHFGPRCSIVPLESSRTDFLPPKFAAQGVDCAAETAYHIVKQ
                     E"
     gene            5950..6366
                     /locus_tag="AC16UZ_03095"
     CDS             5950..6366
                     /locus_tag="AC16UZ_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6245698.1"
                     /translation="MSIVRWNPWREFDDFFTNVAAVPSEHIARSEWLPPVDISETETE
                     YLIDVEIPAVARDDVAVSVKDGVLTVTGERNFEKETDGKRHRVERQYGRFSRSFRLPE
                     NVDEEHISASSKEGVLYLVVSKREKEKPRTIEVKVH"
     gene            6412..7239
                     /locus_tag="AC16UZ_03100"
     CDS             6412..7239
                     /locus_tag="AC16UZ_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0034702 - monoatomic ion channel complex
                     [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HIR6245699.1"
                     /translation="MALDRDTVRVIIFEAHTPAGKAFDVGLIVCILVSVLAVMLDSVG
                     AIHHEYQDVLYGVEWFFTILFTVEYGLRLWCIENTGRYARSFYGIVDLLGIIPTYLSI
                     FFAGTQYLLVIRVLRVLRVFRVLRMMRYVGEAELLTQALRASRRKIIVFISSVAALVI
                     IFGSMMYLIEGEANGFTSIPRSIYWAVITMTTVGYGDITPQTPLGQAVASLVMIMGYG
                     IIAVPTGIVTLELSEASRRAMNTRTCPGCSAEGHTGEATYCWHCGHHLFEPRAVAHD"
     gene            complement(7255..7698)
                     /locus_tag="AC16UZ_03105"
     CDS             complement(7255..7698)
                     /locus_tag="AC16UZ_03105"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6245700.1"
                     /translation="MKVDGRCFCGAVTYDAEIDPDRVLICHCTDCQNHSGSAYRVVAG
                     VVNDSFRLLTGELRSYEKVADSGSLRSRTFCPECGTNIYACTVGEGTGFFGLRLGTIN
                     QRAELTPKLRVWCRSALPWSDDLSGLPAFPAQPDLDALDEIVHQS"
     gene            7871..10549
                     /gene="acnA"
                     /locus_tag="AC16UZ_03110"
     CDS             7871..10549
                     /gene="acnA"
                     /locus_tag="AC16UZ_03110"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR6245701.1"
                     /translation="MSLDTFKTRRTLTVGGREYDYFSLPEAQSAGAGNVEKLPMSLKI
                     LLENLLRFEDDNTVKVEDITALANWASGGAAAEEIAYRPARVLMQDFTGVPAVADLAA
                     MRSAVVSAGLDPTIINPLSPVDLVIDHSVMVDSFGTETAFAENVALEMQRNTERYRFL
                     RWGQKAFDNFRVVPPGTGICHQVNLEYLARTVWTREENGRTVAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPISMLIPEVIGFRLTGKLRDGITATDLVLTVVEMLR
                     RRGVVGKFVEFFGEGLDQLPLADRATIANMAPEYGATCGYFPIDRETIAYLELSGRPA
                     ETVELVEAYARANAMWRDADTPEATYTDVLALDLDSVVPSLAGPKRPQDRVAMKDLGR
                     VFDEALELQGRAGDSDARVPVPGTDYDLGHGDVVIAAITSCTNTSNPAVMLGAGLVAR
                     KALARGMQVKPWVKTSLAPGSKVVTEYLEKTAVQEDLDALGFNLVGYGCTTCIGNSGP
                     LPEHISAAIKQGDLVVSSVLSGNRNFEGRVHQEVRANWLASPPLVVAYAIAGTTRIDL
                     TRDPIALDDQGREVFLRDIWPSNEEIADLVAQVSAEMFERQYADVFTGPDAWRAIDVA
                     ESGTYGWENSTYIKQPPFFTVGGDLDQPAEVSDARILALLGDSVTTDHISPAGAIQPD
                     SPAGQYLQQHAVDVADFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGIEGGYTRHVPS
                     GDQVSIYDAAIRYQGEGTPLVVIAGKEYGTGSSRDWAAKGTRLLGVKAVIAESFERIH
                     RSNLIGMGVLPLQFREGEGRASLNLSGEEIVDVRLPDGVTEISPRQDIEVVIKRGTDT
                     RRVTVQSRIDTENEIAYFKSGGILQYVLNNLVREAG"
     gene            complement(10614..12437)
                     /locus_tag="AC16UZ_03115"
     CDS             complement(10614..12437)
                     /locus_tag="AC16UZ_03115"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR6245702.1"
                     /translation="MAVESSLHAINAHAVAVMLLTVSALYLFTRERIPMEVTSVGLLA
                     ILAVGFSIFPFPGIEPTDFFHGLGHEALVAVCALMVVGQGLVRTGALEPVGRLLGKIW
                     SRAPFLSFLITLIVGAVLSAFVNNTPIVVLLLPILISVCLRTSSSPSGILLPMGFATL
                     VGGMATTIGTSTNLLVVSVANDLGLERFGMFDFALPAAIAGCVALLYLWLIAPRMLPK
                     RTIGLEDSAPRLFEAGLHLGDNSPAVDRTLAEAKAMTGGDMQVIRIRRGDTYLVPLPD
                     ANLRAGDILRVRDTPQRLKSYEDVLKARLYSGDHEVDEDHPLSAGNQMLAEVAVVRGS
                     TLDRTNLRFARFLSRYQLAALALHRAGKSVERPIEEIEDVILQQGDILLVQGARDQIA
                     ALKRSTEFLVLDASVALPHTSKAPLALTIVAGVVAAAAFGIMPIAVSAMAGAALLLLT
                     RCLNLGAAIRAISPSVFFIVVASLALGQALIATGATDYVTSLFLSATRGASPTVILSA
                     LMLLLAVLTNMISNNAAAVIGTPIGIGIAAQLGLPAEPFVLAVLFGANMSYATPMAYK
                     TNLLVMSAGNYSFSDFVRVGVPLTIIMWLTLTWVLSSMYGL"
     gene            complement(12578..>14531)
                     /locus_tag="AC16UZ_03120"
     CDS             complement(12578..>14531)
                     /locus_tag="AC16UZ_03120"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HIR6245703.1"
                     /translation="YLEKELDDQQSSHIFENYLGMLDGAKVYFTAADVAAMERYRYKL
                     DDALVRGNLVPAFEIFNRYQERLTDRLEFLLEELALGLKQIDFTAEDQIEIERENAPW
                     PANEAELDDLWRKRLKAAVLSMKLNGKELDEIQTLLTKRYTNRLKQSRQTKSEDAFQL
                     YVNAFATTYDPHTQYFSPRTSQNFNINMSLSLEGIGAVLRSEDEYTSIVELVPAGPAD
                     KSGLVKPADRIVSVGQGDTGPLIDVVGWRLDDVVELIRGPKGSQVRLEIIPGDSEDDT
                     SRVISITRNTVKLEEQAAQKKLLTMERNGQTQKIGVIEIPTFYVDFKAIQQGDPNYKS
                     TTRDVRLLIEELKAEGIDGLVVDLRNNGGGALQEADSLTGLFIKSGPTVQVKSASRRP
                     LVYSDDDNRAVWDGPLAVLVNRLSASASEIFAGAIQDYERGIIIGSQTFGKGTVQTLV
                     PLNRGQLKITAAKYYRVSGQSTQHQGIIPDIEFPEIYDAERIGESALDDAMPWDMIQP
                     AVYPRSHQIQPVLAELNSKHEERAGGDPDFQYFEALAQRSRENNRRTHVSLNEKERVV
                     QKKDEDTWRLDLENQLRVAKGEEPLESIDDLDDREADEDEAEDPFGDPMIRESANILL
                     DYIGLTRQIAYVEDLDKAAGPATAIQ"
BASE COUNT         2553 a         4504 c         4806 g         2668 t
ORIGIN      
        1 catgttgccg gtgtcgggag ccgcgcagtt catggcctcc gaggagagcg ggtaccgccc
       61 cagcacctcg gcgacgtgcg cgtagtcgag gttgttgagt ccggaaccgc gttcgctgtc
      121 cggaagaaaa aagttccaca ggccgcgctc cttggccttt tccttcatct cctccatggc
      181 ctcggtttcg gtccagcgac cgcccgcatc gacctgctcg gcgtaggcgc gctcgttggg
      241 taccacgtaa tcgtgaatgt actgagtaat gtcgtcgatg agcggcttga cgcgctcaga
      301 gatgcctagg tccattgctt ccccctgggt gcccggtgcc cgatgttcga gcgggacagt
      361 gaatatctct attaaaaggc gtgagaattc gatactaata cagaaggtta cgtggtgcta
      421 tttgacaggg gggagtccat tgaccgcgcc cggcgatgcc accgttcggc tcctgaacga
      481 cctgtttggg ggcggtgtga aaagcgccgt cgtgctgatg cgccactcgg ctcgggagtt
      541 cgcgcccggg cgtcacgatc tggacaaccc gctcaccgag gaaggacgtg atctggcccg
      601 acggctcggg gagcggttgc ccgaggacct gacggtgcgc gcgtatgcca gcccgccgga
      661 gcgatgcatg gaaacagccg agctcgtcct ggccggttac gcgtccaccg gcgggcgggt
      721 gactcggcat cgcccggtcg aggcgcttgg cgtcttctat gccctggacc agatgaaaat
      781 gtggcaggtc atgcaatccg ccggaggaat ggtgcccttc ctggaggctt ggttttccgg
      841 tgacgttccc caggatgcgg tgatgccgcc ccggcttgcg gcagcgctgg tggttcgtct
      901 gatgaccgac aagctttcgg ccatggacgg ggcgcgacgg ctcgacgtct gcgtgtccca
      961 cgacatgacc cttcacctcg tccgcgaccg gttgctgggc cagagcgtac gcgacgtcga
     1021 cgtggacttt ctggacgggc tggtcgcctt cgaaagggag ggcgcgcgct ggctggcgag
     1081 ccatcatggc gagcccgtga agctcgatgc gcagctgctc tctctagggg gcctgtggga
     1141 ctgaggagag ctggacgcac gcggcgcccg gcccatggcg tgccctaagt gtcctatgtg
     1201 ccctatgtgc cctatgtgtc ctaaggggcc tcgcctacgt tgaggggcaa tgaggggtcc
     1261 cgcgcccact cggacatgga tccgtcgtag acggccacgt tgtcgtgccc gaggagcagg
     1321 caggcgaagc cgtcaagggt ggcggcgatg cccccgccgc agtaggtcac gacccggtcg
     1381 gcatcgagca gccccgtgga ttcgagcgcc tcggcaagcg cgccggccgg cttgaagcgt
     1441 ccgcccgcca gcagttcttc gtagggcacg ttcacgctgc ccggaatgtg gcctggccgg
     1501 ccgtagttcg tggttccggt gcccgcgtat acctccggtg agagcgcgtt gaccgtgcag
     1561 acacccggct cgtccatcgc tcggcacatc ccgtcccgat cggtgaacag gctggcgtct
     1621 ggtcgggggc ggtaggtcac cgctgctcgc ctcgcggtgc cggtctcgaa cggaagcccc
     1681 agatcgcgcc acgcgtcgaa cccgccgttg aggacggacg ctcgctgatg gccgacgtag
     1741 cgcagcagcc accatgcacg ggtagcccac atgaggtggc ccgtcgagta gacgaccacc
     1801 tcgctgtcgt cggagatgcc caggcggccg agcgcgtcgg cgatgacccc cggatccggc
     1861 aggctgaagc cgaggcccgt ggtcgtatcg gagaacgcgt cgatgaggtc catgaacgcg
     1921 gcgccgggta tgtgcccctg ctcgtagcgt tcgcgcccgc tctccgctcg gtagccgcgc
     1981 tcggcaggaa cgaggaaaac ggtggcatcg acgatccgca gatcggcatc gtccagccgc
     2041 gcggcgagtg ccgcgggttc ctctagcagc tcaggacgtg ggtatgccat ttgattctcc
     2101 tgctggtttg tcggtttggt gctggacggc ctcatcccgg ctcgtcctct ccgcgggcgt
     2161 gccgcgctag acggatggcc cgttctccgg ggagtcgatc tgggcgatgg catcgttggt
     2221 cagcagcacg aagatcagga agctggtgtc cgcggccagc tgctcctcga cgagcatctc
     2281 gtatgcccgt tcccggagcg atcgcagctg ggcgagcgcc gctgcccgct cttcgctgcc
     2341 cgcgccctct gccacggagt cgcgggttcg gatcacctgc gtgtagaagg cgtcgatccg
     2401 gttctttctg cggacctgat agatcttgag gatggcgaac gctccgctca ccatgccgac
     2461 cagcagcgtg accagcacct cggcgacgcc ggagtaacgc tcgatgagcg aaggctcgtc
     2521 gcgctgcagg tagtcgagcg cgccgggatg caggctgaag gtccagtggg cgcgggcaat
     2581 gtcgtcgtct atgggctgaa agagctccgg gcgcttgctg aagagcgcgg gacgcaggcg
     2641 gagaaactcc tcgaacagat cgaacaccag cgtctcgtcg aggctttccc tggcaaccag
     2701 cagggtatcg acggcaaagg tcaccacagg gtccggcgtg agcgatccgt aggttcctgc
     2761 cgggatgaca tagggccgca gcgtcgggtt cagcagcacc gttcgatcga cgatggagcc
     2821 tggcctggtg tcgccggggt ccccgaatga gaagagcttg aggctttcaa ggctcggatt
     2881 gctcatgacc cgcctccggt cgatgggcgc aaacaggatg ataacgtcgg gggccgcgtc
     2941 ctgatcggac gtgaacgata agcccttttc gagctgcaac ccgctcacga tccgctcgcc
     3001 gatctggcgg ggtatcgaac ccggcggccc ggcaaatacc cgagccccgt cgagcagttc
     3061 ctccaggctg ccgtcagcgc gatcggcgat cgagccgacg tggagcacgc tcggatagat
     3121 gggcatgatc gtggtgatgg tgtcgcgatg ctcgttggta ttcggcgcga acgcgatgtc
     3181 gccgtaaccc tgctccagcg cgtcgaggac cgagagggag tcgtcaggta acgggatcag
     3241 attgatcttc aggccggagt cctcgtccac caggtccgcg atcttgcgcg cgatgatctg
     3301 ctcgacgggc agctcgggca tgatcagatc gacctcggtc accgaagggg agcagccggc
     3361 gaggttggtt ccggtgaagc cgagcatcag cgccgcaaag gcagcgagcc cgggctcgcg
     3421 tttactcatc gagcttagag ccggcatgcc agccgaagtt gatctcgagg cgcatccggg
     3481 caccgagcac tgtatcccag tcgcgctcct ccacccagcc gaccaccggc cagaactcga
     3541 caaacatcca gggccgccag aaattccggc ggtaacgaat gcgcagcttc acttgctggt
     3601 atttgtctct tggcccaatc aggatctcct cgtcgtcgta gatcgagtcc tcagccgtgt
     3661 cctggcgcca tatcgcctcg taggcgatgg ctgaccggtc gctgatgttc tgaaagagtg
     3721 aaaacttctg ctccagctgg gggtttgagt ccttgtcctc gaagtattcc aggcgggacc
     3781 gcgagcggaa caggaagcgc tcgccgatgg gcctgttgaa gtagatgcgc aggtcgtccc
     3841 gccagccgtt gcgggccagg tagtaaaggc gattggttgc ctggccgcgc caattctcgg
     3901 agatccggta tttgtagctc gtgttgacgc ggacgaaggg gtcgacctcg ccgcctcgga
     3961 agcttacccc cccgtcgacg ctgatgcccg tcttcttgcc cttgcgggcc acccatcgca
     4021 gcgcgacgtc gttctcgtcg tcctccagct ccaccccctc gtcctcctcg tcaccgccag
     4081 cgtcgttgac gaccaggcgg accctttcac cgagcccggg aagatgaaag ttgaccttga
     4141 cccggggcga aaactcccag ccgtgatgct cgatgtagtc tgtgtccaga cgcagccgaa
     4201 ggcgatttcg atcctcctcg aacgtctcct gggcctcggt caggaaaaac gcgtcgaacc
     4261 ggcgcacggc ggcgtcgatc atccctgcgg tcgatgtgtg aagatcgtct acttttgtcg
     4321 gtcctggctc gacctgatcc gcgtcgggcg ggggggtgtc gttcgccgtc cgaccgggtt
     4381 gggcagatga gtcaacgggc tcggccgatg cgctcagcgg cagggccatg agcccggcga
     4441 ggaggattgt cgagactgaa gtccagggga atctcaagac ggttcgtggc tgcgtttcat
     4501 gaggtagtag cccacggaaa gcacgagcac cagcgcggcg atacccagca cggtaacgcc
     4561 gtcgaccttt gcgtagtcga tcacgatgat cttgcgcgat acggcgatca gagcaatcag
     4621 catcacgagc tccacgtgga cgatgtcgtc ttcgaggtac atcttcacgc tggccagcag
     4681 ctcgagcccg atcaggacca gaaggaagaa accgaagatg ccgagcagct cgttgatgtc
     4741 gagaaggaga taggcgggct cttccgaaaa gtggttctca agggctgcct ggacgatgat
     4801 gattgccagt tcgatcgaac tcgccaccac cacgagcagc atgagcacga gtagaccgag
     4861 aaccacccag cgttcgatgt tggcaatgag ctgcatcata tgaaaagcca tcccgcgccg
     4921 cttagccagg gcgacgatta gattagagag ggcccggggc gatgtccaat cggcgtccgt
     4981 cggcgttggc gggcagcggc cggagaaacc accgccgtgg atccgctgcg cgggccccaa
     5041 agtggttgcc gcaggcgacg gcattggcca gaatgcggtc aacagggagg taaacatcgc
     5101 cgggggcgca gcgcctggcg gtgtagaaga ataacgatgc agatccgctt ctggcgagac
     5161 cgccggccct ggcggccggt cagcatgcac cagagcgccc ctaacccgtg cttggaaagg
     5221 cattggggcg gtctcaggct ggagcatggc tccaccgtgt tcgtgcccat gtgcggtaag
     5281 agcgacgacc tgcgatggct gcttggccgg ggccatcagg gtctcggcat cgagctggcg
     5341 gacctcgcta tcgaagctta cttcgaagag gctggggagc cttacgagcg ggaggattcg
     5401 gaccggctcg tccgctatcg tggtcctcga acggacatcc tctgtggcga cttcttcgcg
     5461 ttgacggccg ccgagctctc cgctgcgcgc gccgtcctgg atcgcggcgc gctgatcgcg
     5521 ctgccgccga agatgcgagc ccgctacgtc gatcacctgc tcagggtcat tcccgaccgc
     5581 accgacatcc tgctcgtcac cctcgagtac gatcagaatc tggtggcggg cccgccgttc
     5641 tcggtgctgc cggaggaagt ggtttcccat ttcgggccgc gctgcagcat cgtgccgctg
     5701 gagagttccc gcaccgactt cctgccgccc aaattcgccg ctcagggtgt ggactgcgcg
     5761 gcggaaacgg cctaccacat cgtcaagcag gaatagcgtt ccccatcccg ggccggtcga
     5821 cggtcggcga gcagcagttt tgttgccggg tggggatcaa ttttctcttt tcaagggctt
     5881 ttaaattttc cggcagcgcc catttatcgg gaatcgttgt ttgaactgag tggttaggag
     5941 ccaactgaaa tgagcattgt ccgatggaac ccctggcggg aattcgacga tttcttcacc
     6001 aacgtggccg cggtgcccag tgagcacatc gctcgttcgg agtggctgcc gcccgtcgac
     6061 atctctgaaa cggagacgga atacctcatc gacgtcgaga tcccggcagt tgcccgggac
     6121 gacgtcgccg tctcggtgaa ggatggcgtg ctgacggtaa ccggcgagcg aaacttcgag
     6181 aaggagaccg atggcaagcg tcatcgggtg gaacggcagt acgggcgatt cagccgcagc
     6241 ttccggctgc ccgagaacgt ggacgaggaa cacatctcgg cttcgtccaa ggaaggcgtt
     6301 ctctatctgg tcgtctccaa gcgggagaag gaaaaaccgc gcaccatcga ggtaaaagtc
     6361 cactgacgat ggcttgctga cccgacgtcg cgccgtggtt taatacgcgc catggcgctc
     6421 gaccgagata ccgtccgggt catcatcttc gaggcccaca cgcccgccgg caaggcgttc
     6481 gatgtgggcc tcatcgtctg catcctcgtc agcgttctcg cggtgatgct cgattccgtc
     6541 ggggcaattc accacgagta ccaggacgtg ctctacggcg tggagtggtt cttcaccatc
     6601 ctattcaccg tggagtacgg cctgcgcctg tggtgcatcg agaataccgg ccggtacgcg
     6661 cgcagcttct acggtatcgt cgacctcctc ggcatcatcc ccacctatct gagcatcttc
     6721 ttcgccggca cccagtatct gctggtgatt cgggtgttgc gggtgctgcg ggtattccgg
     6781 gtactgcgca tgatgcgcta cgttggcgaa gcggagctgc tgacccaggc cctgcgcgcc
     6841 agccgtcgga agatcatcgt tttcatctct tcggttgcgg cgctggtcat catcttcggc
     6901 tccatgatgt atctcatcga gggggaggct aacggcttta ccagcatccc gcgcagtatc
     6961 tactgggccg tgatcaccat gaccacggtg ggatacggcg acataacgcc gcagacgcct
     7021 ctgggtcagg cagtcgcctc tctggtcatg atcatgggtt acggcatcat tgcggtgcct
     7081 accggcatcg tgactctgga gctcagcgaa gcttcacgtc gtgccatgaa cacgcgcaca
     7141 tgtccgggat gctccgccga agggcacacg ggagaagcga cctactgctg gcactgtgga
     7201 caccatctgt ttgagccgcg ggccgtggcg cacgattgac cgcacgggtc gaggtcagct
     7261 ctgatggacg atctcgtcca gggcatccag atccggctgg gcagggaagg ccggtaaccc
     7321 tgacaggtcg tccgaccatg gcagcgccga gcggcaccag actctgagct tcggcgtcag
     7381 ctcggcccgc tggttgatgg tgcccagcct gagaccgaag aaaccggtac cttcgcccac
     7441 cgtgcaggcg tagatgttgg taccgcactc ggggcagaag gtcctcgagc ggagcgagcc
     7501 gctgtcggca accttctcgt acgatctcag ctcgccggtc agcaaccgga agctgtcgtt
     7561 caccactccg gccacgacac ggtaggcgct gcccgagtga ttctggcagt cggtgcagtg
     7621 gcagatgagg acccggtcgg ggtcgatctc ggcgtcgtag gtcacagcgc cgcagaagca
     7681 gcgtccgtcg actttcatga aggcatcctc ccaggcgaat gtcccgccaa gactaccaga
     7741 gctgcgcgct gcgggcgtat ccacgggttt acgccccgct tcgcgcccta caaaacactg
     7801 ctcaggagcc gggtaacgag tacaataccg cccctttttc ctggcaccga aaacaacgag
     7861 cgaggcagcc atgagcttag ataccttcaa gacccgcagg acgctcaccg tcggcggtag
     7921 ggaatatgac tatttcagcc tgccggaagc gcagtccgcc ggggctggga acgtcgaaaa
     7981 gctcccgatg tcgctgaaga tcctcctaga aaacctgctc aggttcgagg acgacaatac
     8041 cgtcaaggtg gaggacatca cggcgctggc caactgggca agcggcgggg cggcggccga
     8101 ggagatcgcc tatcgcccgg cgcgggtgct catgcaggac ttcacaggtg tgccggcggt
     8161 ggcggatctg gccgccatgc gcagcgcggt ggtgtccgcc ggcctggatc ccacgatcat
     8221 caatccgctg tctcccgtgg acctggtcat cgatcactcc gtgatggtcg acagcttcgg
     8281 aaccgagacg gcgtttgcgg agaacgtcgc cctggaaatg cagcggaaca ccgagcgata
     8341 ccggttcctg cgctggggcc agaaggcgtt cgacaatttt cgcgtggttc cgccgggcac
     8401 cggcatctgt caccaggtga acctggaata tctcgccaga accgtctgga cccgggaaga
     8461 gaacgggcgc accgtggcct accccgacac tctggtcggc accgacagcc acacgacgat
     8521 gatcaacggc ctgggcgtgc tgggttgggg cgttggcggt atcgaggcgg aggccgccat
     8581 gctggggcag ccgatctcca tgctgattcc cgaggtgatc gggttccggc tcaccggcaa
     8641 gctgcgcgac ggaatcacgg ccaccgacct cgtgctcacc gtggtcgaga tgctccgccg
     8701 caggggcgtc gtgggcaaat tcgtcgagtt cttcggagag ggcttggacc agcttccgct
     8761 cgcggaccgg gcgaccatcg ccaacatggc ccccgagtat ggcgccacct gcggctattt
     8821 ccccatcgat cgggagacca tcgcctatct cgagctttcg ggtcggccgg cggaaacggt
     8881 ggagctcgtc gaggcgtacg cacgggccaa cgcgatgtgg cgggatgccg acaccccgga
     8941 agccacctat accgacgttc ttgccctgga tctggactcc gtggtgccga gcctggcggg
     9001 gcccaagcgc ccccaggatc gtgtggccat gaaggatctg ggcagggttt ttgacgaagc
     9061 gctggagctt cagggccgcg cgggtgactc cgacgcacgt gttccggtcc cgggcacgga
     9121 ctacgacctc ggccacgggg acgtggtgat cgccgccatc acctcctgca cgaacacgtc
     9181 aaaccccgcg gtcatgctgg gtgccggcct ggtagcgcgc aaggcgctcg ctcgcggcat
     9241 gcaggtcaag ccctgggtga agacgtcgct ggcgccgggt tcaaaggtcg ttaccgagta
     9301 tctggagaag acggccgtcc aggaagatct ggatgccctc gggttcaacc tcgtgggcta
     9361 tgggtgcacg acgtgcatcg gcaattccgg gcctctgccg gagcacatct cggccgccat
     9421 aaagcaaggg gatctggtgg tgagctcggt gctgtcgggt aatcgcaact tcgagggtcg
     9481 cgtccatcag gaggtcaggg ccaactggct ggcttcgccc ccgctggtgg tggcctacgc
     9541 catcgctggc acgacgcgca tcgatttgac ccgcgacccg atcgccttgg acgaccaggg
     9601 cagagaggtg tttctccggg acatctggcc gagcaatgag gaaattgccg atttggtggc
     9661 ccaggtctcc gcggagatgt tcgagcgtca gtacgcggac gtgttcaccg gtcccgatgc
     9721 ctggcgtgcc atcgacgtgg cggaatcggg cacgtacggc tgggaaaact ccacgtacat
     9781 caagcagccg ccattcttca cggtgggcgg tgatctcgat cagccggccg aggtgtcgga
     9841 cgcccgtatt ctggcgctgc tcggcgactc ggtgaccacc gaccacatct cgccggcggg
     9901 cgccattcag ccggacagcc cggcgggtca gtacctgcag cagcacgccg ttgacgtggc
     9961 ggacttcaac tcctacggct cgcgtcgcgg caatcacgag gtgatgatgc gcggtacctt
    10021 cgcgaacatc cggatccgca acgagatggt ccccgggatc gaagggggct acaccaggca
    10081 cgtgccgtca ggggaccagg tgagcatcta cgatgccgcg atccggtatc agggcgaggg
    10141 aacgccgctg gtggtcatcg ccggcaagga gtacggcacc ggatcgagcc gggactgggc
    10201 cgccaagggc acccggctgc tgggcgtcaa ggccgtgatt gccgagagct tcgagcgcat
    10261 ccaccggtcg aacctcatcg gcatgggcgt gctgccgctc cagttccgcg agggtgaggg
    10321 ccgcgcgtcc ctcaatctct cgggagagga gatcgttgac gttcgtctgc cggacggcgt
    10381 gaccgagatc tcgccgcgcc aggacatcga ggtggtgatc aagcgcggga cggatacccg
    10441 gcgggtgacc gtgcagagca ggatcgacac ggagaacgag atcgcctact tcaagagcgg
    10501 cgggatcctt cagtacgtgc tgaacaacct ggttcgcgag gcaggctgac ggttgctcga
    10561 agagcgctcc ggccggcggc gcgccgtgcg cgcacggccc cagggcgagg cgctcacaac
    10621 ccgtacatgc tgctcaatac ccaggtgagc gtgagccaca tgatgatggt cagcggtacc
    10681 cccacccgga cgaagtcgga gaagctgtag ttgccggcgc tcatgacgag caggttggtc
    10741 ttgtaggcca tcggcgtggc atagctcatg ttcgcgccga agagcacggc aaggacgaag
    10801 ggctccgccg gtagccccag ctgcgcggcg atcccgatcc caatgggcgt gccgatgacg
    10861 gctgccgcgt tgttcgagat catgttggtg agcaccgcca gcagaagcat cagcgcggac
    10921 aggatgaccg tcggcgatgc cccccgggtc gcgctcaaga aaagcgacgt cacgtagtct
    10981 gtcgccccgg tggcgatgag ggcctggccg agcgccaggc ttgccacgac gatgaagaac
    11041 accgaggggc tgatggctcg gatggccgcc cccagattca gacaccgcgt gagcagcaac
    11101 agcgccgcac ccgccatggc gctcacggcg atcggcatga tcccgaaggc ggccgcggcg
    11161 accaccccgg cgacgatggt cagcgccagc ggggccttgg acgtgtgcgg cagagcgacc
    11221 gacgcgtcca ggacgagaaa ctcggtgctg cgctttaagg ccgcgatctg atcccgtgcg
    11281 ccctggacca gcaggatgtc tccctgctga aggatcacgt cctcgatctc ctcgatgggc
    11341 cgctccactg attttccagc ccggtgcagg gcgagcgcgg ccagctggta gcgcgacagg
    11401 aagcgggcga acctcaggtt ggtgcggtcc agggtcgacc cgcgaaccac cgcgacctcg
    11461 gccagcatct ggtttccggc tgaaagcggg tggtcttcgt ccacctcgtg atcgccggag
    11521 tagagccgtg ctttcagcac gtcctcgtag gacttgagcc gctggggcgt atccctgacc
    11581 cgcagaatgt ctcccgcgcg caggttggcg tcgggcagag gcacgagata cgtgtcgccc
    11641 cggcggattc gaatgacctg catgtcgccg ccggtcatcg ccttcgcctc ggccagcgtg
    11701 cggtccaccg cgggcgagtt gtctcccaga tgcagtccgg cctcgaatag ccggggcgcc
    11761 gagtcctcca ggccgatggt ccgcttcggc agcattcggg gggcgatcag ccacagataa
    11821 agcagggcta cgcagcccgc aatggccgcg ggcagcgcga agtcgaacat cccgaaacgc
    11881 tccaggccca ggtcgttcgc cacgctgacc accagcaggt tcgtggacgt gccgatggtc
    11941 gtcgccatgc cgcccaccag ggtggcgaaa cccatgggaa ggagaatgcc ggatggcgac
    12001 gatgacgtgc gcaggcagac gctgatgaga atgggcagca gcagaaccac gatgggcgtg
    12061 ttgttcacga acgccgacag cacggcgccg acgatgagcg tgatcagaaa cgacaggaac
    12121 ggagcgcggc tccagatctt gccgagcaat cgacccacgg gctcgagcgc ccctgtccgg
    12181 accaggccct ggccgaccac catcagggcg cagacggcca ccagcgcttc gtggccgagc
    12241 ccgtgaaaga aatcggtggg ctcaatgccc gggaagggaa aaatggagaa tccaactgcg
    12301 aggatggcga gcagaccgac gctggtcacc tccatgggta tgcgctcgcg ggtgaagaga
    12361 tagagcgcgc tgaccgtgag cagcataacg gcgaccgcgt gagcgttgat cgcatgcagg
    12421 ctgctttcta cagccacttc aggcatcgtg cgtctgacaa gaaagcaact tagatgacgt
    12481 ccacgagaag gtcggagaag tgtacgtcta ttctggggtc aaagcctgca gaaatctctc
    12541 aatattggcc cggagcgtcg ggtcgcccgg cgattttcta ctggatggcc gtcgcgggcc
    12601 cggcagcctt gtcgaggtct tccacgtagg cgatctgccg ggtgaggccg atgtagtcga
    12661 gcagaatgtt ggcgctctcg cgaatcatgg gatcgccgaa aggatcctcc gcttcgtcct
    12721 cgtccgcctc ccgatcgtcc aggtcgtcga tggattccag cggctcttct cctttggcga
    12781 cccggagctg attctccaga tccaggcgcc acgtgtcctc gtccttcttc tgcaccacgc
    12841 gctctttctc gttcagggag acgtgggtgc gacggttgtt ctcgcggctg cgctgggcga
    12901 gggcttcgaa gtactggaag tcaggatcgc cgcccgcacg ctcttcgtgc ttggagttga
    12961 gctccgcaag gaccggctga atctgatgtg atcgcgggta cacggcgggc tggatcatgt
    13021 cccagggcat ggcgtcatcg agggcgcttt cgccgatacg ctccgcgtcg tatatctccg
    13081 ggaactcgat gtcgggaatg atgccctggt gctgggtgct ctggccggac acccgatagt
    13141 acttggccgc ggtgatcttc agctgacccc ggttcaaggg gaccagggtc tgcaccgtgc
    13201 ctttgccgaa ggtctggctg ccgatgatga tgccgcgctc gtagtcctgt atggcgcccg
    13261 cgaagatctc cgaggccgaa gcggagagac gattgaccag caccgcgagg gggccgtccc
    13321 acacggcccg gttgtcgtcg tccgaataga ccaggggccg tcgcgaggcg gatttgacct
    13381 gcacggtggg cccggacttg atgaacagcc cggtgagcga atccgcttcc tgcagggcgc
    13441 caccgccgtt gttgcgaaga tcgacgacga ggccgtctat gccctccgcc ttgagctcct
    13501 cgatcagcag ccgcacgtct cgcgtggtgc tcttgtagtt gggatcgccc tgctgaatgg
    13561 ccttgaagtc gacatagaac gtcgggatct cgatgacgcc tatcttctgg gtctggccgt
    13621 tgcgctccat ggtgagcagc ttcttctggg cggcctgctc ctcgagcttc acggtgttcc
    13681 gcgtgatgct gatcacgcgg ctggtgtcgt cctccgagtc gccggggatg atctccagcc
    13741 tcacctggga gcccttgggg ccacggatga gctccacgac gtcgtcgagc cgccagccca
    13801 ccacgtcgat cagcggtccg gtatcgccct ggcccacgct gacgatccgg tcggccggct
    13861 tgaccagccc ggatttgtcc gcgggcccgg cggggacgag ctcgacgatg gacgtgtact
    13921 cgtcctccga gcgcagaacg gcgccgatgc cttccaggga aagcgacatg ttgatgttga
    13981 agttctgcga cgttcgcggc gagaagtact gggtgtgcgg gtcgtaggtg gtcgcgaagg
    14041 cgttcacgta gagctgaaac gcatcttcgc tcttggtctg ccggctctgc ttgagccggt
    14101 tggtgtagcg cttggtcagc agggtctgga tctcgtcgag ctctttgccg ttgagcttca
    14161 tgctgagcac cgctgctttt agccgcttgc gccacagatc atccagctca gcctcgttcg
    14221 cgggccacgg ggcgttctcg cgctcgatct cgatctggtc ctcggcggtg aagtcgatct
    14281 gcttcagacc cagcgccagc tcttccagca ggaactccag ccggtcggtc aggcgctcct
    14341 ggtaccggtt gaagatctcg aaggcgggca cgaggttgcc ccgcaccaga gcatcgtcca
    14401 gcttgtagcg atagcgctcc atcgccgcca cgtcggcggc ggtgaagtac accttggcac
    14461 catccagcat gccgagatag ttctcgaaga tgtggctcga ctgctggtcg tccagttcct
    14521 tttcgaggta g
//
LOCUS       DBNHOX010000146         4787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1168843, whole genome shotgun sequence.
ACCESSION   DBNHOX010000146 DBNHOX010000000
VERSION     DBNHOX010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1168843"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="AC16UZ_03125"
     CDS             complement(<1..460)
                     /locus_tag="AC16UZ_03125"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase family protein"
                     /protein_id="HIR6245704.1"
                     /translation="MRKLLVLTVVVIFVAVLGWQKRVELVVWGAPKLLALLQPVGENV
                     PVEWATGPEFPVATPGDRPPNVILILTDDMGFNDISLYNGGAGDGSVMTPNIDALARQ
                     GVTFTNGYAANAVCAPSRASIMTGRYSTRFGFEFTPFPRIGATLFRWSRDV"
     gene            571..1611
                     /locus_tag="AC16UZ_03130"
     CDS             571..1611
                     /locus_tag="AC16UZ_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6245705.1"
                     /translation="MATREPLLLKGAPGSPYTRKMLAYLRYRHIPYELLIGDQTARLK
                     LPEPKVSLLPTFYLPDEKGELQAVVDSTPLIRRFEIEFPDRQAIPADPVLAFLNYLIE
                     DYADEWLTKAMFHYRWHYDADIEMAGTILPRWTNIQASEAALEKFKAFVSDRQISRLY
                     VVGSNDVTAPVIEASYLRFLEIMNELIERQKFVLGKRPSSADFGIYAQLTQLAKFDPT
                     PARICLDRAPRVYAWTDLVDDLSGHPAEDDGWMDRADASDTLSDLLTEIGRTYAPALL
                     ANAEALRNADEHMEAIIDGQRWEQPTFPYQGKCLQWINAEYRKLDPSARADVDRILQG
                     TGCEAIVLEPDT"
     gene            1615..2658
                     /locus_tag="AC16UZ_03135"
     CDS             1615..2658
                     /locus_tag="AC16UZ_03135"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6245706.1"
                     /translation="MRKLLYAVLIIVVLLATAAAVVYNTPGGQDALFERAAKAVMGQT
                     PQPLDGMRVVVCGSASPLGNDPNRAQACIAVVTPEHFFLFDVGARSPVRMAQARLPMG
                     RIDGVFLTHFHSDHIAALPDVNLASWVQSRPAALTVYGPPGVGAVVDGFNTAYRLDRG
                     YRTAHHGAELLPPERGPMLAKTFVPGDVVWRDDLMTVTSFEVEHPPIEPAVGYRVDYR
                     GRSVVISGDTNAADTLFTVAKDADLLLHDALSRTLLDPMIATATELGVPVLPTIMTDV
                     IDYHADVTSLPARAAEAGIRQLALYHMVPVPPNALAAKMFLRGIPDDVIVTRDLHTFD
                     LPANSTDIVISEP"
     gene            2662..3948
                     /locus_tag="AC16UZ_03140"
     CDS             2662..3948
                     /locus_tag="AC16UZ_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR6245707.1"
                     /translation="MKLKTRLRSAAMRQLSSAAYLEKQRAKARKLRQRKGEPPVVHYF
                     HQVDDPYSHLAVQKLDELDDRYDLAFQPHLASKPGEDYRGSAAHFDHWARKDAASVAA
                     DYGTRFAPAINAPAGDAVDAANRVLSAHLDRDDFARAAVSIGEALWSDQRIAEPADAH
                     DGLRAVAEGNDLRRALGHYQGAMFYFEGEWFWGVDRIRLLERRLIDEGFGSSETLCVP
                     EPTPTETSGLDTSSVLLEYFPSLRSPYTAIGHARVLDLIARSGVCVRVRPVMPMMMRG
                     IPAPRAKQRYIITDAGREGRAHGAPFGPIVDPFGDPVKRAFALLPGAAAAGREMEYVT
                     AYLNAAWFEGVDITTQKGLRAVATNAGMDWAALEQASRQENWEAILEENLNTMLKEDL
                     WGVPSFRISGGNSDEAFACWGQDRIWRVENEIASRT"
     gene            4009..4647
                     /locus_tag="AC16UZ_03145"
     CDS             4009..4647
                     /locus_tag="AC16UZ_03145"
                     /inference="COORDINATES: protein motif:HMM:NF037008.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HIR6245708.1"
                     /translation="MRAAEKLFAERGIENVSIRDIVGEAGQKNESALQYHFKSLTGLI
                     SAIHAERSAETQARRGELLEAMLAQTPDPPLRQLCILMVRPAFDLARGDVGFRRYIKA
                     FGQELALTETSALARAVAHGGGGTSGQQLAGLLKRALPHLDEEAYQRRMEAAVRLCSA
                     SVFHQARQRHAFRGQQADLFFHSLIDALVGLLSAPVSQETRAVAESLGRPEA"
BASE COUNT          893 a         1581 c         1506 g          807 t
ORIGIN      
        1 cgacatcccg gctccatctg aaaagcgtgg cgccgatccg cgggaacggc gtgaactcga
       61 atccgaagcg ggtcgagtac cggccggtca tgatgctggc ccgggacggg gcacagaccg
      121 cgttcgccgc gtagccgttg gtgaaggtga ctccctggcg ggcaagcgcg tcgatgttcg
      181 gtgtcattac cgagccgtct ccggcaccgc cgttgtacag cgagatgtcg ttgaagccca
      241 tgtcgtcggt caggatgaga atcacgttcg ggggccgatc gccgggcgtc gcgacgggga
      301 actcggggcc tgtggcccac tctacgggaa cgttctcccc caccggctgc agcagcgcga
      361 gcagcttggg cgcgccccag acaaccagct ctacccgctt ctgccagcca agcacggcca
      421 caaagatcac cacgaccgtc agaacaagga gctttctcat cgtgtcagga ccaccgggca
      481 tcggacgtgg gaacgaatga tacacaccaa ataatgtttg tcattatatt tgtgaggcgc
      541 tatcctgact catcttcaga gagaagactc atggccacac gcgaaccgct cctgctcaaa
      601 ggggcacccg gctcccccta cacccgcaag atgctggctt acctgcgcta tcggcatatc
      661 ccctacgaac tgctcattgg cgatcagacg gcccggctga agctgcccga gccgaaggtg
      721 agcctgctgc cgaccttcta tctgccggac gagaagggcg agctccaggc agtggtggac
      781 tccacgccgc tcatccgccg cttcgagatc gaatttcccg atcgccaggc gatacccgcg
      841 gatccggtgc tggcctttct gaactatctc atcgaggact atgcggacga gtggctcacc
      901 aaagccatgt tccactaccg ctggcactac gatgcggaca tcgagatggc gggaaccatc
      961 cttccccgct ggaccaacat tcaggcttcg gaggcggcgc tcgagaaatt caaggcgttc
     1021 gtctccgatc ggcagatctc gagactttac gtggtcggct cgaacgacgt caccgccccg
     1081 gtgatcgaag caagctacct gcgattcctg gagatcatga acgagctgat cgagcggcag
     1141 aagttcgtgc tcggcaaacg tccttcctcc gcggatttcg ggatctacgc ccagctcacg
     1201 cagctggcca agttcgaccc gacgcccgct cgaatctgcc tggatcgggc gcccagagtc
     1261 tatgcctgga cggatctcgt cgacgatctc agcggccacc cggcggaaga cgacgggtgg
     1321 atggatcgcg cagacgcctc ggatacgctc tcagacctgc tgaccgagat cggtcgcacc
     1381 tacgcgccgg ccctgctggc gaacgccgag gcgctgcgca acgcagacga gcacatggaa
     1441 gccatcatcg acgggcaacg ctgggagcag ccgaccttcc cctatcaggg caagtgcctg
     1501 caatggatca atgccgagta ccggaaactc gatccgtcgg cacgagccga cgtcgaccgc
     1561 atactgcagg gcaccggctg cgaagccatc gtcctcgaac cggatacgta gatcatgaga
     1621 aaactactgt acgcagttct gatcatcgtc gtgctcctcg ccacggcggc cgccgtcgtc
     1681 tacaacaccc ccggcggcca ggacgcgttg ttcgagcgcg ccgccaaggc cgttatgggc
     1741 cagaccccac agcctctgga cggcatgcgc gtggtcgtgt gcggcagcgc gtcgccgctc
     1801 ggcaacgatc cgaatcgggc tcaggcatgc attgccgtcg ttaccccgga gcactttttc
     1861 ctgttcgacg tgggcgcgcg ctcgccggtc aggatggctc aggcgcgatt gcccatgggc
     1921 cggatcgacg gggtgtttct gacgcacttc cattccgatc acatcgccgc gctgcccgac
     1981 gtgaacctgg cctcctgggt gcagagcaga ccggccgcgc tgacggtgta cggcccgccg
     2041 ggagtcgggg cggtggtgga cggattcaat accgcttacc ggctagaccg cggctaccgc
     2101 acggctcacc acggcgccga gctgttgccg cccgaacgcg gcccgatgct ggcgaaaacg
     2161 ttcgtccccg gcgacgtcgt ctggcgcgac gacctgatga cggtgacctc gttcgaagtg
     2221 gagcatccgc ccatcgagcc cgcggtgggc taccgcgtcg actatcgggg acgctcggtg
     2281 gtgatctccg gagataccaa tgcggcggac accctgttca ccgttgccaa agacgcagac
     2341 ctgctgctgc acgacgcgct gtcgcgtacg ctgctggacc cgatgattgc caccgccacc
     2401 gagttgggcg ttccggtcct tccgaccatc atgaccgacg tcatcgacta tcacgccgac
     2461 gtcaccagcc tgcctgcgcg cgcggcggaa gccggcatca ggcagctggc cctgtaccac
     2521 atggtgcccg tcccacccaa cgcgctggcg gcaaagatgt tcctcagggg cattccggac
     2581 gacgtcatcg tcacgcggga tctgcatacc ttcgatttgc ccgcgaactc cacggacatc
     2641 gtcattagcg agccttgagc catgaaactc aaaacccgac tgcgctcggc tgccatgcgc
     2701 cagctctcct cggccgccta tctggagaaa cagagggcaa aggcgcgaaa gctgcggcag
     2761 aggaagggcg agccgccggt cgtgcactac ttccaccagg tggacgatcc ctacagtcac
     2821 ctggcggtgc agaagctcga cgagctagac gaccgctacg atctcgcttt tcaaccccac
     2881 ctggcttcca agccaggcga agactaccgg ggcagcgccg ctcacttcga tcactgggca
     2941 cgcaaggacg ccgcaagcgt ggccgcggat tacggcaccc ggttcgcgcc cgccatcaac
     3001 gcgcccgccg gcgatgccgt ggatgcggca aaccgggtgc tgtcagctca cctggatcgc
     3061 gacgatttcg cccgcgctgc cgtcagcatt ggcgaggcgc tgtggagcga tcagagaatc
     3121 gccgagccgg ccgacgctca cgacggcctc cgggccgttg ccgaaggaaa cgacctgcgc
     3181 cgcgcgctcg gacattacca gggagccatg ttctacttcg aaggggaatg gttctggggt
     3241 gtcgaccgaa ttcgtctgct ggagcgaagg ctgatcgacg agggcttcgg cagcagcgag
     3301 accctttgcg tgcccgagcc gaccccaacg gaaaccagcg gcctcgacac cagttctgtg
     3361 ctgctggagt acttcccgtc tctgcgcagc ccctacacgg ccatcggtca cgcccgcgtg
     3421 ctcgacctca tcgcacgcag cggggtttgc gttcgagtgc ggccggtgat gccgatgatg
     3481 atgcggggca ttcccgcgcc gcgcgcgaag cagcgctaca tcatcacgga cgccggccgc
     3541 gaagggagag cacatggcgc gccgttcgga cccatcgtcg atccgttcgg ggatccggtg
     3601 aagcgtgcct ttgctctgct ccccggtgcg gccgcagccg gccgggagat ggagtacgtc
     3661 accgcatacc tcaacgccgc ctggttcgag ggcgtggaca tcacaacgca gaaaggcctg
     3721 agagcggtgg cgacaaacgc gggaatggat tgggccgcgc tcgagcaggc cagccggcag
     3781 gagaactggg aagccatact ggaggaaaac ctcaacacga tgctcaaaga agacctttgg
     3841 ggcgtgccga gttttcgaat ctcgggcgga aacagtgacg aggccttcgc gtgctgggga
     3901 caggaccgca tctggcgggt cgagaacgaa atcgcgagcc gaacgtagat gccggcgcag
     3961 agccaagcgg gtagacactc gagaagcagg gccacccagg acgcgctgat gcgcgccgcg
     4021 gaaaaactgt tcgcggaacg aggcatcgag aacgtgtcga ttcgcgacat cgtgggcgaa
     4081 gcgggtcaga agaacgagtc cgcgctgcag taccatttca agagcctcac cggcctcatc
     4141 tctgccattc acgccgagcg atcggcggaa acccaggccc ggcgcggaga gctgctcgaa
     4201 gcgatgctgg ctcagacacc cgaccccccg ctacgccagc tctgcattct gatggtcagg
     4261 cctgcattcg acttggcccg cggtgacgtg ggtttccgtc gctacatcaa agcgttcggg
     4321 caggagctgg ctctcacgga gacctcggct ctggctagag cggtcgccca tggcggaggt
     4381 ggaaccagcg gacagcagct ggccgggctg ctgaaacggg cgctgcctca cctggatgag
     4441 gaggcctatc aacgccggat ggaggcagcg gtgcgcctgt gttccgcttc cgtgttccat
     4501 caggccagac agcggcacgc ctttcgcggc caacaggcag atctgttctt ccacagcctc
     4561 atcgacgcgt tggtggggct actgagcgcg cccgtttccc aggagacccg ggcagtcgcc
     4621 gaatccctcg ggcggcccga ggcgtgaggt ttgggtcgcg caatgctgcg gcccggcgct
     4681 gacaggctgt cgtgacgagg atcgtctccc gcgctgtcct tgccctcgtc gtcgttaccc
     4741 taggcgcgtt ctgcttcgcg ttcttctccc cccgcccagc cctgacc
//
LOCUS       DBNHOX010000147         2886 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_76058, whole genome shotgun sequence.
ACCESSION   DBNHOX010000147 DBNHOX010000000
VERSION     DBNHOX010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_76058"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="AC16UZ_03150"
     CDS             complement(<1..517)
                     /locus_tag="AC16UZ_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6245709.1"
                     /translation="MSYARLVQSALKGAQLLRKLGLKTGDGVAVVAENHPNTLAFFWA
                     AQMAGLYYTPISVQFHPDEIGYILADCDARLLMASRAQEDRVAGAPATLIADACEGAE
                     MLYSSGTTGRPKGVRAASPGAPLGTVSELFRRRLALHEVDGQAVYLSTAPLYHSAPLR
                     YNTMMHRCGATS"
     gene            complement(589..924)
                     /locus_tag="AC16UZ_03155"
     CDS             complement(589..924)
                     /locus_tag="AC16UZ_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HIR6245710.1"
                     /translation="MSDDQKHIYKVLFHNQGQIYEVYAHNIYQSDLYGFIEVEDYIFG
                     KKTQVVIDPAEDKLRTEFDGVQRSFIPMHAVIRIDEVEREGVAKVTESSGGNITPFPV
                     PMPKDTGKS"
     gene            1017..2348
                     /gene="miaB"
                     /locus_tag="AC16UZ_03160"
     CDS             1017..2348
                     /gene="miaB"
                     /locus_tag="AC16UZ_03160"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR6245711.1"
                     /translation="MMKKLYIKTHGCQMNEYDSARMADLLRESQGYELVAEPEDADLI
                     LLNTCSIREKAQEKVFHQLGRWRALKVRKPGLVIGVGGCVASQEGAAIQSRAPHVDVV
                     FGPQTLHRLPEMIDAAAGPARVPLVDVSFPEIEKFDRLPQPRAEGPSAFVSVMEGCSK
                     YCSFCVVPYTRGEEVSRPVADVMREVIQLAGQGVREVNLLGQNVNSYLGPIDDDHADL
                     AELIYYVAEVDGIDRIRFTTSHPLDFNDSLVRAFADVPELVDHLHLPVQSGSDRVLAL
                     MKRGHTVSEYREKIAALKEVRGGISLSSDFIVGFPGETEADFQATMDLVAEIGFDTSF
                     SFVYSARPGTPAAALPDDTPEAVKRERLALLQGLLRSQADAIACDMVGTRQTVLVTGE
                     SRKNREELQARTENNRVVNFPCPDRRLVGGFVDVDITEALPNSLRGVMAIT"
     gene            2441..>2886
                     /locus_tag="AC16UZ_03165"
     CDS             2441..>2886
                     /locus_tag="AC16UZ_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR6245712.1"
                     /translation="MSEDDSRVVALTTNVSLEPNEPRRLAALCGPFDQNLKHLERRLG
                     VHIRSRGNLFQISGPEQRVQAAGALLSQLYRETLEHETIDPDMVHLFLQESGVDELLA
                     RQRGPDEMPADGEGAVISTGRKSVKPRGGNQTRYVERIRGHDVNFG"
BASE COUNT          503 a          902 c          966 g          515 t
ORIGIN      
        1 cgctggtggc gccgcagcgg tgcatcatgg tgttgtagcg cagcggcgcg gagtgataca
       61 gcggcgcggt ggagaggtag acggcctggc cgtccacctc gtgcagcgcc agccggcgcc
      121 tgaacagctc ggagaccgtg ccgagagggg cgcccggact tgccgcgcgc acgcctttgg
      181 gccggcccgt ggtgccggag gagtagagca tctccgcgcc ctcgcaggcg tcggcgatca
      241 gggtcgcggg cgcgccggcc acccgatcct cctgggcgcg gctcgccatc agcagccggg
      301 cgtcgcagtc agcgaggatg tagccgatct cgtcgggatg aaactggacg ctgatggggg
      361 tgtagtagag ccccgccatc tgtgccgccc agaagaatgc cagggtgttc gggtggttct
      421 ccgcgaccac cgcgacgccg tcgcccgttt tcaggccgag cttgcgcagg agctgcgcgc
      481 ccttcagcgc gctttggacc agccgcgcgt agctcacgcg ctgatcgccg aagatccatg
      541 cggtcttgtc gggctcgacc tccgcccagt ggagcgggtg aaggccgctt acgatttgcc
      601 ggtgtccttg ggcatgggaa ccgggaaggg ggtgatgttg ccgccgctgg actccgtgac
      661 tttcgccacg ccctcgcgtt cgacctcgtc gatgcggatc acggcatgca tggggatgaa
      721 gctgcgctgc acgccgtcga actccgtgcg cagcttgtcc tcggccgggt cgatgaccac
      781 ctgggtcttc ttgccgaaga tgtagtcctc cacctcgatg aagccgtaga ggtcgctctg
      841 atagatgttg tgcgcgtaga cctcgtagat ctgaccctga ttgtgaaaca gcactttgta
      901 gatgtgcttc tggtcgtcgc tcattggcgt tgatacctgc tgtgccagat gtcctatata
      961 gaggtgtatg tggaaaattc aagcatttat aatcccgcgc cccgaatccg ggtcgtttga
     1021 tgaagaagct ctacataaaa acccacggtt gccagatgaa cgagtacgac tcggcccgca
     1081 tggccgatct gctgcgggag agccagggtt acgagctcgt cgccgagccg gaagacgcgg
     1141 acctgatcct gctgaacacc tgctcgatcc gcgagaaagc ccaggagaaa gtctttcacc
     1201 agctgggtcg gtggcgcgcg ctcaaggtgc gcaagcccgg gctggtgata ggcgtcgggg
     1261 ggtgcgttgc cagccaggaa ggcgcggcca tccagagccg ggcgccccac gtggacgtgg
     1321 tattcgggcc gcagaccctg caccggctgc ctgaaatgat cgatgccgcc gcgggtcccg
     1381 cacgggttcc gctcgtggat gtgagctttc cggagatcga gaagttcgac cgtctgcccc
     1441 agccgcgggc tgaagggccc tcggccttcg tctccgtgat ggagggctgc agcaagtact
     1501 gcagcttctg cgtggtgccg tacacccgcg gagaggaggt cagccgaccg gttgccgacg
     1561 tgatgcgcga agtgatccag ctcgccggtc aaggggtccg ggaggtgaac ctgctcgggc
     1621 agaacgtgaa ttcctacctg ggccccatcg acgacgacca tgcagacctg gcggagttga
     1681 tctactacgt ggccgaagtc gacggcatcg accgcatccg cttcaccacc tctcatccgc
     1741 tggacttcaa cgattcgctc gttcgcgcgt tcgccgacgt tccggaactg gtggatcatc
     1801 tgcatctgcc cgttcagtcc gggtccgacc gggtgcttgc tctgatgaag cggggccaca
     1861 ccgtgagcga gtatcgtgaa aagatcgcgg ccctcaaaga ggtgcgaggg ggcatcagcc
     1921 tttcgtcgga cttcatcgtc ggcttccctg gcgagaccga ggcggacttc caggccacca
     1981 tggacctcgt cgccgagatc gggttcgata cctcgttcag cttcgtctac agcgcacgcc
     2041 ccgggactcc ggcggccgcc ctgccggacg acacgccgga agccgtcaag cgcgagcgcc
     2101 tggcgctcct gcagggcctg ctgcgctctc aggcggatgc catcgcctgc gacatggtcg
     2161 gaacgcggca gacggtgctg gtcacgggcg agtccaggaa gaatcgggaa gagctgcagg
     2221 cgcgcaccga gaacaatcgg gtcgtcaact tcccctgtcc ggaccgtcgc ctcgttggcg
     2281 gcttcgtgga cgtggacatt accgaagccc tgccaaactc cctgcggggc gtgatggcga
     2341 tcacataggg cgcagacgcg cgtttggggc ggcgaaaccc gatttgacag gtaactgcgc
     2401 gccgatatcc tagacaaccc tcttctgcac agggcgcgca ttgagcgaag acgattcacg
     2461 ggtggtggcg ctcaccacga atgtcagcct ggaacccaac gaaccccgcc gattggcagc
     2521 cttgtgcggg ccgttcgacc agaacctcaa acacctcgag aggcggctgg gcgtacatat
     2581 tcgcagccgc ggcaacctat tccagatcag cgggccggag cagcgtgttc aggcggccgg
     2641 cgcgctgctg tcccagctat accgggagac gctcgagcac gagaccatcg acccggacat
     2701 ggttcacctg ttccttcagg aatcgggcgt cgacgagctg ctcgcgcgtc agcgcgggcc
     2761 agacgagatg cccgcggacg gggagggtgc ggtcatctcg accgggcgaa aatcagtcaa
     2821 gccccggggc ggcaaccaga cccgttacgt cgagcggatt cgcggccacg atgtgaattt
     2881 cggcat
//
LOCUS       DBNHOX010000148         1603 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_76117, whole genome shotgun sequence.
ACCESSION   DBNHOX010000148 DBNHOX010000000
VERSION     DBNHOX010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_76117"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="AC16UZ_03170"
     CDS             <1..667
                     /locus_tag="AC16UZ_03170"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6245713.1"
                     /translation="WDDITFVATFPFKNNEGIDQGDAYDISALNPLNKVPTLVTGEGQ
                     VIYGSQAACEYLDGTSRARRMYPEPGPARWDAVTRLALSDTIFEQTVVMVMEQWKPES
                     EWNRGLFEWLWPKFIAGLDKLERDAERGWGDFDVGHAAMLHAISYLGFRAEFYEAKDP
                     IHPDFRWRDGRPALSAWFDEAVERPSVQSHYLKDFVGDSSAEFCQAQVQAVLAAQKQH
                     GTG"
     gene            664..1365
                     /locus_tag="AC16UZ_03175"
     CDS             664..1365
                     /locus_tag="AC16UZ_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6245714.1"
                     /translation="MIDLYYWPTPNGHKVAIMLEEIGSEYQLHPVNILEGEQFDPDFL
                     EISPNNRIPAIVDRSGPDGTPYALFESGAILLYLAEKTGVLWPSSTAARYDMLQWLFF
                     QNANVGPMFGQCGHFQGYAPEPVPYAIERYHNETRRLYGVMDRRLASCAYLGGPAYSL
                     ADIATYPWCMPIIRELHKIDIDEFPNVADWADRIAQRPAVARGTSLLADVMKIGDPDE
                     KTREAFFGSRQLEQR"
     gene            complement(1377..>1603)
                     /locus_tag="AC16UZ_03180"
     CDS             complement(1377..>1603)
                     /locus_tag="AC16UZ_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6245715.1"
                     /translation="ALRNVQMLLDQAGSGLDKLIKVTIYLTDMTQFPKIVELRRKWFT
                     PPYPADTIVEVRSLALPELMIEIDAIALAG"
BASE COUNT          304 a          524 c          513 g          262 t
ORIGIN      
        1 ctgggacgac atcaccttcg tcgccacgtt tcccttcaag aacaacgagg gcatcgacca
       61 gggcgacgcc tacgacatct cagcactcaa ccctctgaac aaagtgccga ccctggtcac
      121 cggggaaggc caggtcatct acggcagcca ggcggcctgc gaatatctcg acggcaccag
      181 cagggcccgc cgcatgtacc ccgagcccgg cccggcgcga tgggacgcgg tgacgcgcct
      241 ggcgctcagc gacaccatct tcgagcagac ggtggtcatg gtgatggagc agtggaagcc
      301 ggaaagcgag tggaaccgcg gccttttcga gtggctgtgg cccaagttca tcgccgggct
      361 ggacaagctg gagcgggacg ccgagcgggg ctggggcgat ttcgacgtcg gccacgcggc
      421 tatgcttcat gcgatcagct atctgggatt cagggccgaa ttctacgagg ccaaggatcc
      481 gatccatccc gatttcaggt ggcgcgacgg ccgaccggcg ctgtccgcct ggttcgacga
      541 agccgttgag cgtccctcgg tccagtccca ctacctgaag gatttcgtcg gcgactcgag
      601 cgccgagttc tgccaggcac aggtgcaggc cgtgctggca gcgcagaaac agcacggcac
      661 gggatgatcg acctctacta ctggccgacg cccaacggcc acaaggtcgc catcatgctc
      721 gaggagatcg gcagcgagta ccagctgcac ccggtcaaca tcctcgaggg ggagcagttc
      781 gatcccgact tcctcgagat cagccccaac aaccgcatcc ccgccatcgt ggaccgcagc
      841 ggacccgacg ggacgcccta cgcgctgttc gaatccggcg cgattctgct ctacctggcg
      901 gagaagacgg gcgtcctgtg gccgtcgagc acggcggcga ggtacgacat gctgcagtgg
      961 ttgtttttcc agaatgccaa cgtcggcccc atgttcgggc agtgcggcca cttccagggc
     1021 tacgcgcccg aaccggtgcc ctacgccatc gagcgctatc acaacgaaac ccgacggctc
     1081 tacggcgtga tggaccggcg cctggcgagc tgcgcctacc tcggggggcc ggcctactcg
     1141 ctggcagaca tcgccaccta tccctggtgc atgccgatca tccgggagct gcacaagatc
     1201 gacatcgatg agtttcccaa cgtggccgac tgggcagacc gcattgcgca gcgcccggcc
     1261 gtggcgcggg gcacgtctct gctcgcagat gtcatgaaga tcggcgaccc ggacgagaaa
     1321 acgcgcgaag ctttcttcgg atcccgacag ctggagcaac gctgagggga agcgcctcag
     1381 ccggccaggg cgatggcgtc gatttcgatc atcagctccg gcagggccag cgaacggacc
     1441 tcgacgatgg tgtccgccgg gtacggcggc gtgaaccact ttcgccgcag ctccacgatc
     1501 ttcgggaact gcgtcatgtc ggtcagatag atggtcacct tgatcagctt gtcgaggccg
     1561 gagcctgcct gatcgaggag catctgaacg ttgcgcagcg cct
//
LOCUS       DBNHOX010000149         2012 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_590676, whole genome shotgun sequence.
ACCESSION   DBNHOX010000149 DBNHOX010000000
VERSION     DBNHOX010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_590676"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..898)
                     /gene="rarD"
                     /locus_tag="AC16UZ_03185"
     CDS             complement(2..898)
                     /gene="rarD"
                     /locus_tag="AC16UZ_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HIR6245716.1"
                     /translation="MSEQRAGMLFALAAYGFWGFAPVYFKVLDFAGPVEIIAHRVLWS
                     VPLLALLIGARRQFGALLALRADQVGWLGLSGLLVAVNWGVFVWALLNGRMLETSLGY
                     YINPLVSVLLGVLFLGEWLRRGQLVAVALAAIGVVNEVVSVEVIPWAGLTLAVSFGFY
                     GLVRKRLGVDSAVGLGVEATLLLPVALGYLAYQFEAGQGALNRGEGGEIAWLAASGLV
                     TTFPLVCFAAAALKLPLSTLGFVQYLAPTITFFLAVLVFGEAFRWEQSVTFGCIWLAV
                     VIFSVEGVYHQRRLQRRLGRWA"
     gene            complement(926..1282)
                     /locus_tag="AC16UZ_03190"
     CDS             complement(926..1282)
                     /locus_tag="AC16UZ_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6245717.1"
                     /translation="MKYVRIYSGDDGQSHFEDVEVDLADHGPLGRISDLVRGKGVVFR
                     EVEGEYHYDFHNAPRRQFVVNLTGSVDIEVGDGTVRRMEPGSILLAEDLTGQGHVSRS
                     VNGEPRTCLFIPLADD"
     gene            1389..>2012
                     /locus_tag="AC16UZ_03195"
     CDS             1389..>2012
                     /locus_tag="AC16UZ_03195"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6245718.1"
                     /translation="MDQTSNHVLREIVDILNVERIETNLYRGQNHQTEHVFGGQVLAQ
                     AIAAAYRTVDVPHGLHSIHAYFLRPGDWRVPILYEVDRIRDGRSFSTRRVVAVQNGRA
                     IFSMASSWQKDEEGLEHAVQMPDVPPPGALRGDREAYLEAAREHPEISRFAFRFEAID
                     SRQVERILMTDSGTHPPYKHTWLRTRETLPDDPEIHLAMLAYMSDLDF"
BASE COUNT          418 a          717 c          595 g          282 t
ORIGIN      
        1 gtcaggccca gcggccaaga cgccgctgca gcctgcgctg gtggtagacg ccctcgacgg
       61 agaagatcac cacggccaac cagatgcagc cgaacgtgac gctctgctcc cagcgaaacg
      121 cctcgccgaa cacgaggacg gccagaaaga acgtgatggt gggcgccaga tactgaacga
      181 agcccagggt cgacagcggc agctttagcg ccgccgccgc aaagcagacc agtgggaagg
      241 tcgtgacgag cccgctggca gccagccacg cgatctcgcc gccttcgcca cggttgaggg
      301 caccctgccc cgcctcgaac tgatacgcca ggtaccccag cgccacgggc agaagcaggg
      361 tggcctccac gcccagcccc acggccgagt ccactcccag ccgcttacgg accagcccgt
      421 agaagccgaa gctcaccgcc agggtcaggc ccgcccaggg gatgacctcc acggagacga
      481 cttcgttcac cacgccgatg gcggcgagag caaccgccac cagctgcccg cgacgcagcc
      541 actcgccgag aaacagcacc ccgagcagaa cggagaccag cgggttgatg tagtacccaa
      601 ggctggtttc gagcatgcgc ccgttgagca gagcccagac gaacacgccc cagttcacgg
      661 ccacgagcag gccgctcaac ccaagccagc caacctgatc cgcacgaagc gcgagcagcg
      721 cgccgaactg gcgccgcgcg ccgatcagca gcgcgagcag aggaaccgac cagaggaccc
      781 tgtgcgcgat gatctccacc ggcccggcaa aatccagaac cttgaagtaa acgggagcaa
      841 agccccagaa gccgtaagcg gccagcgcga acagcattcc cgcgcgttgc tcgctcatgt
      901 cggtttccgg aatggcagag acgattcagt cgtctgcaag cggtatgaaa agacaggtgc
      961 gaggctcgcc attgacgctc cggctgacgt gcccctgccc ggtgagatct tcggcgagca
     1021 ggatgctgcc gggctccatg cgccgaacgg tgccatcacc gacttctata tccacggacc
     1081 ccgtcaggtt gaccacgaac tgccggcgcg gagcattgtg aaaatcgtag tggtactcgc
     1141 cttcgacttc gcggaacacc acccccttgc cgcgcaccag atccgagatc ctgcccaggg
     1201 gcccgtgatc ggcgagatcc acctctacgt cctcgaaatg cgattggccg tcgtccccgc
     1261 tgtagatcct cacgtatttc acgtcgctgc ctccgtagtg tccccacccg accccgatcc
     1321 gggcatctca tcgggtgcca tgtccagtag aatgccacca accgtgcggc gcagccatcg
     1381 acccgaccat ggatcagacc agcaaccacg tgcttcgaga gatcgtcgac atcctgaacg
     1441 tcgagcggat cgaaacgaac ctctaccgcg gacagaacca tcagaccgag cacgttttcg
     1501 gcggccaggt gctggctcag gccatcgccg ccgcgtaccg caccgtggac gtaccccatg
     1561 gcctgcactc catccacgcc tatttcctcc gccccggtga ctggcgggtg ccgatcctgt
     1621 acgaggtgga ccggatccgg gacggccgca gcttctccac gcgccgggtg gtagccgtcc
     1681 agaacggacg ggccatcttc agcatggcgt cctcgtggca gaaagacgag gaaggactag
     1741 agcacgccgt gcagatgccc gacgtgccgc cgcccggcgc tctgcgcgga gaccgtgagg
     1801 cgtacctgga agccgcgcgc gagcacccgg aaatcagccg tttcgccttt cgtttcgaag
     1861 ccatcgacag caggcaggtg gagcggatcc tgatgaccga ctcgggaacc cacccgccgt
     1921 acaagcacac ctggctgcgg acccgcgaga ccttgcccga cgatcccgag atccatctcg
     1981 ccatgctcgc ctacatgtcc gacctggact tc
//
LOCUS       DBNHOX010000150         2899 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1683704, whole genome shotgun sequence.
ACCESSION   DBNHOX010000150 DBNHOX010000000
VERSION     DBNHOX010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1683704"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..431)
                     /gene="yajC"
                     /locus_tag="AC16UZ_03200"
     CDS             complement(90..431)
                     /gene="yajC"
                     /locus_tag="AC16UZ_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR6245719.1"
                     /translation="MNLFETTAYAADAGGPPPDAGMINLLFLGGFILIFYFLLWRPQS
                     KRRKEHQALMSGLAKGDEVVTTGGIVGQVTRVDDDFVKLQVANNIELRVQKSSVGATL
                     PKGTLKSLDGD"
     gene            complement(418..1533)
                     /gene="tgt"
                     /locus_tag="AC16UZ_03205"
     CDS             complement(418..1533)
                     /gene="tgt"
                     /locus_tag="AC16UZ_03205"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR6245720.1"
                     /translation="MSFELLGTDGAARRGRLKTAHGTVETPVFMPCGTYGTVKAMTPR
                     ALKEVGTQMLLGNTFHLMLRPGERLIESLGGIHEFMHWDGPVLTDSGGFQVWSLGELR
                     KISEEGVVFRSPVNGDRVELTPERSVAVQRALGSDVLMVFDECTPYPATEAEARLSME
                     LSMRWAERSLRAHQPSSGGLLFGIVQGGMFPALRQASLESLVELDFPGYAIGGLSVGE
                     SKSEMREVLDRLLPLMPTTKPRYLMGVGTPEDLVRAVGLGVDMFDCVMPTRNARNGYA
                     FTSAGTLKIRNARHRESDLPLDERCGCYTCANFSRAYLHHLDKCGEMLGSMLMTHHNL
                     HYYHWLMAELRASIESGKFRTLIETLRTDWNSPDEPI"
     gene            complement(1523..2545)
                     /gene="queA"
                     /locus_tag="AC16UZ_03210"
     CDS             complement(1523..2545)
                     /gene="queA"
                     /locus_tag="AC16UZ_03210"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR6245721.1"
                     /translation="MHPGDFDYELPNGQIAQAPASRRDASKLMHIAGDGIEHLGFHRF
                     PELLMPGDLLVLNDTRVIKARLHGVKDSGGQAEVLVERIEGETEALCQVRVSKALKPG
                     RFIQIGDAELEVLGRKGDFYSLRFPSDVLAFLEQRGETPLPPYIERAAGTVDADRYQT
                     VYGRRPGAVAAPTAGLHFTEDLLRQVRDRGVEIAEVTLHVGAGTFQPVRVDDISQHRM
                     HAERYEICSRAARALAECRERGGRIVAVGTTVVRTLESAAARSGEVEAGSGETDLFIT
                     PGFPFRVVDVLLTNFHLPRSTLLMLVCAFAGYERVMAAYAEAVDEGYRFFSYGDACFM
                     ERTDEL"
     gene            2675..>2899
                     /locus_tag="AC16UZ_03215"
     CDS             2675..>2899
                     /locus_tag="AC16UZ_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245722.1"
                     /translation="MIIRHMNRSYNRQEAEAEIRDRGLHEMVFEVPAVDNEPHWHDFD
                     AYLYLLDGTLHLRDVQAGVVHECSPGMRIDV"
BASE COUNT          545 a          955 c          894 g          505 t
ORIGIN      
        1 ggcgccttcg acaacccgag caggccttgt cccaccacac cactcgaatt agccacggct
       61 ggacgttctc cgcgcttgcc agccgcgcac taatcgccgt ccagggattt cagcgtgccc
      121 ttaggcagcg tcgcgccgac ggagctcttc tgcacgcgca gctcgatgtt gtttgccacc
      181 tggagcttga cgaaatcgtc atccaccctg gtcacctgac caacgatgcc gcccgtggtg
      241 acaacctcat cccctttggc caggcccgac atcagcgcct gatgttcctt gcggcgcttg
      301 ctctgagggc gccacaggag aaagtagaag atgagaatga aaccgccgag gaaaagcagg
      361 ttgatcatgc cggcatcggg cggcggaccg ccggcatcgg ccgcgtaggc agtggtctca
      421 aataggttca tccggggagt tccagtcggt tcgaagggtc tcgatgaggg tgcgaaattt
      481 accgctttcg atggaggcgc gcaattcagc catcagccag tggtagtagt ggaggttgtg
      541 gtgcgtcatc agcatgctgc ccagcatctc gccgcatttg tccagatgat gaagataggc
      601 ccgcgagaaa ttcgcgcagg tgtagcagcc gcagcgctca tcgaggggca ggtccgattc
      661 ccgatgcctg gcgttgcgga tcttcagggt tcccgccgac gtgaaggcgt agccgttgcg
      721 cgcgttgcgc gtgggcatca cgcaatcgaa catgtccacg ccgagcccca ctgcacgaac
      781 gaggtcctca ggcgtgccga cccccatgag atatcttggt ttggtcgtgg gcatcagcgg
      841 caacagcctg tctagaacct ccctcatctc cgacttgctc tcccccaccg agagcccgcc
      901 gatggcgtaa ccggggaagt cgagctcgac cagtgactcc aagctcgcct gacgcagagc
      961 cggaaacatg ccgccctgga cgatcccgaa gaggagacca ccggacgagg gttggtgcgc
     1021 acgcaggctg cgctccgccc agcgcatcga cagctccatg gacaggcggg cctcggcttc
     1081 cgtcgcgggg taaggcgtgc actcgtcgaa caccatcaaa acgtcgctgc ccagagcgcg
     1141 ctggacggca accgagcgct cgggcgtaag ctcgacacga tcgccgttca ccggcgagcg
     1201 gaaaaccacg ccctcctcgc tgatcttgcg cagctctccc aggctccaaa cctgaaagcc
     1261 accgctgtcg gtgaggactg gaccatccca gtgcatgaac tcgtgaatgc cgcccaagga
     1321 ttcaatgagc cgttctccgg ggcgcagcat cagatgaaag gtattgccga gcagcatctg
     1381 cgtccctacc tccttcagcg cgcgcggcgt catggccttg acggtcccgt aggttccgca
     1441 gggcatgaaa accggtgtct cgaccgtgcc gtgcgccgtt ttcaggcgcc cgcgccgggc
     1501 agcgccgtcg gtgccaagca actcaaagct catcggttcg ctccatgaag caagcatctc
     1561 cgtagctgaa aaagcgatat ccctcgtcga ccgcctccgc ataggcggcc atcacccgtt
     1621 cgtaacctgc aaacgcgcag acgagcatca gcagggtgga ccgcggcagg tggaaattcg
     1681 tgagcagcac atccaccacg cgaaacggaa atccgggcgt gatgaacaga tcggtctcgc
     1741 ccgagcccgc ctcgacttcg ccgcttcgag ccgcggcact ttcgagggtg cggaccaccg
     1801 tggtgcccac ggcgacgatc cggcctccgc gctcccggca ctcggccaga gcacgagccg
     1861 cgcggctgca gatctcgtac cgctcggcat gcatccggtg ctgcgagatg tcgtccaccc
     1921 gaacgggctg gaacgtcccg gcgcccacgt ggagagtgac ttcggcgatc tccacccctc
     1981 ggtcgcgaac ctgccggagc agatcttccg tgaagtgcag gccggcggtg ggcgccgcca
     2041 ccgcgccggg ccggcgtccg tatacggtct ggtagcgatc ggcatccacg gtcccggccg
     2101 ctcgctcaat gtagggcggc agcggcgttt cgccgcgttg ttccaggaat gcgagcacgt
     2161 ctgacggaaa gcgcaggctg tagaaatccc cttttcgacc gagcacctcg agttcggcat
     2221 cgccaatctg gatgaaccgg cctggtttga gcgccttgct gacccggacc tgacacagcg
     2281 cctcggtttc gccctcgatt cgctcgacca gcacctcggc ctgcccgccg ctgtccttga
     2341 cgccatgcag ccgcgccttg atgacacggg tgtcgttgag cacgagcaga tcgccgggca
     2401 tcagcagctc gggaaaccga tgaaagccga ggtgctcgat cccatcgccg gcaatgtgca
     2461 tgagcttgct ggcgtctctc ctggacgcgg gagcctgggc aatctgcccg tttgggagct
     2521 catagtcgaa gtcgcctggg tgcattggtt ttaccgacca acggaactgc gcaggccgcg
     2581 gattctaatc cggcgcgtgc aggagcaaaa gcgcgccctg ctggggcata atggcagcgc
     2641 tttcagccac gctcacggag gcccgggaaa tcagatgatc atcaggcaca tgaaccgctc
     2701 gtacaaccgg caggaagccg aggcggaaat tcgcgaccgg ggtctccacg agatggtctt
     2761 cgaggtcccc gccgtcgaca acgagccgca ctggcacgat ttcgacgcct atctctacct
     2821 gctcgatggc acgctgcacc tgcgggacgt gcaagccggc gtggtgcacg agtgctcccc
     2881 gggcatgcga atcgatgtg
//
LOCUS       DBNHOX010000151         2665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_526519, whole genome shotgun sequence.
ACCESSION   DBNHOX010000151 DBNHOX010000000
VERSION     DBNHOX010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_526519"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2665)
                     /locus_tag="AC16UZ_03220"
     CDS             complement(<1..>2665)
                     /locus_tag="AC16UZ_03220"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6245723.1"
                     /translation="KQAKIITLYPGATAQEVQDEVTYHIEEALQLMGQLKRIKMSISR
                     PGMSDLAIEFKDEYKADDFPDIYDELRRKLQDMHGKLPPGVQGPRVIDDFGDVYGIYA
                     ALTGDGYTYRDLKDAADELKKQLVLVPGVRKIVIGGGQREVVYIEISRTRISELGIPL
                     EQIANILQSQNMVTDAGRVRVGPDYVRIHPTGEFTSVQAIGDVLIGSNERRLIFLKDI
                     ATIHRAYEEVANKLIFFNGKPALTLGVSIQAGENVVAVGEAVRKRFGQLASTIPIGMD
                     IKVIYNQPEEVQKSVSGFIVSVGQAIAIVIAVLLVFMGLRTGIIIGAVLLITVSGTLL
                     FMQLYGIELQRISLGALVIALGMLVDNAIVVAEGMLVRIQAGMNPTKAAEETVGKTMW
                     PLLGGTIIGILAFSAIGLSTDNTGEFASSLFYVILISLTLSWVTAVSTTPLLCAMFLK
                     PDPDADPDANPYDKGFFRTFRGALKVVIRLRVLAVAVVVGLFVLAGFGFGYIKNAFFP
                     NSNTPMFFVDIWEPEGTDIRVTRRDAQAISEFLRAQEGVRSTVALIGGGDARFALTYE
                     PKESSPAYAQIIVQTETLEQISKIWPAVEAHLQEHYPHLDPIIKAFRIGPGRDGKIEA
                     RFHGPDPAVLRSLAERAKAIMREDPGAKEINDDWRQPVMLVRPVFNERVARQLGVTRE
                     NVATALKSAYEGTDVGVYRDGIRMLPIKLRPPAEERADVANIQDIRVWSPALQTAVPI
                     AQVVNSFETVFENTILRGRNRIQTIIAACNPTGELATPLFERLKPRIEDIELPPGYEL
                     SWGGEYEDSANAQAGLARVLPAGFILMILTSILLFGKVRQPLIIWLTVPLAIIGITAG
                     LLGFDGAFDFMSLLGALSLIGLLIKNAIVLI"
BASE COUNT          461 a          828 c          879 g          497 t
ORIGIN      
        1 tcgatcagca cgatggcgtt cttgatgagg agcccgatga ggctcagcgc gccgagcagt
       61 gacatgaagt cgaacgcgcc gtcgaaaccg aggagccctg cggtgatgcc gatgatggcg
      121 agcggcaccg tgagccagat gatcagcggc tggcggactt taccgaacag caggatgctg
      181 gtgaggatca tgaggatgaa gcccgcgggc agcacccggg cgaggccggc ctgggcgttg
      241 gccgagtcct cgtactcgcc gccccaggac agctcgtagc ccggcggcag ctcgatgtct
      301 tcgatccggg gcttgagccg ctcgaacagc ggcgtggcca gctcccccgt ggggttgcag
      361 gcggcgatga tggtctggat gcggtttcgg ccgcgcagaa tcgtgttctc gaacacggtc
      421 tcgaacgagt tcactacctg agcgatggga accgccgtct gcagcgccgg gctccatacc
      481 cggatgtcct ggatgttcgc cacgtcggcg cgttcttccg ccggcgggcg aagcttgatc
      541 ggcagcatgc ggatgccgtc ccggtagacg cccacgtccg tcccttcgta ggcggacttg
      601 agcgcggtgg cgacgttctc ccgcgtgacg ccgagctgcc tcgcgacccg ctcgttgaac
      661 accgggcgca ccagcatgac gggctgccgc cagtcgtcgt tgatctcctt ggcccccgga
      721 tcctcgcgca tgatggcttt cgcccgctcg gccagggagc gcaacaccgc aggatccggg
      781 ccatggaatc gcgcctcgat cttgccgtcg cgccccgggc cgattcgaaa ggccttgatg
      841 atgggatcca ggtgcgggta gtgctcctgc aagtgggcct ctaccgccgg ccagattttc
      901 gagatctgct ccagggtctc ggtctgcacg atgatctggg cgtaggcggg gctggattcc
      961 ttgggctcgt aggtcagcgc aaaccgggcg tcgccgccgc cgatcagggc gaccgtggat
     1021 cgcacgcctt cctgggctct caagaattcc gagattgcct gggcgtcgcg tcgggtgacg
     1081 cgtatgtcgg tgccctcggg ttcccagatg tcgacgaaga acatgggcgt gttcgagttc
     1141 gggaagaagg cgttcttgat gtacccgaag ccgaaacccg ccagcacgaa cagaccgacc
     1201 accacggcca ccgcgagcac ccgcaggcgg atgaccacct tcagcgcgcc gcggaaggtt
     1261 ctgaagaaac ccttgtcata ggggttcgca tcaggatcgg catcgggatc cggcttcaag
     1321 aacatggcgc acaggagggg cgtcgtgctg accgccgtca cccaggacag cgtcagggag
     1381 atcaggatca cgtagaacag ggagcttgcg aactcgcccg tgttgtctgt cgacaggccg
     1441 atggcggaaa acgcgaggat gccgatgatg gtgccgccga gcagcggcca catggtcttg
     1501 ccgaccgttt cctcggcggc cttggtgggg ttcatgccgg cctggatgcg aacgagcatg
     1561 ccttccgcta ccacgatggc gttgtccacc agcatgccca gcgcgatcac cagggcgccc
     1621 agggaaatcc gctgcagctc gatgccgtag agctgcatga agagcaacgt gccggagacg
     1681 gtgatcagca gcacggcgcc gatgatgatg ccggtgcgca gccccatgaa gaccagcagc
     1741 acggcgatca cgatggcgat cgcctgcccg acgctgacga tgaagccgga taccgacttc
     1801 tgcacttcct cgggctggtt gtagatgact ttgatatcca tgccgatggg tatcgtgctc
     1861 gccagctgcc cgaatcgctt tctcacggcc tcgcccacgg cgaccacgtt ctcgccggcc
     1921 tggatggaca cgcccagggt cagcgccggc ttaccgttga agaagatgag cttgtttgca
     1981 acctcctcgt aggcccggtg aatggtcgcg atgtctttga ggaaaatcag acgacgctcg
     2041 ttcgaaccga tgagcacgtc gccaatggcc tggacggacg tgaactcgcc ggtgggatga
     2101 atgcggacgt agtcgggtcc gacccgaacc cgtcccgcgt ccgtgaccat gttctgggac
     2161 tgcaggatgt tggcgatctg ctccagcggt atgccgagct cgctgatccg cgttcgtgag
     2221 atctcgatgt agaccacctc gcgctggccg ccgccgatga cgatcttcct cacgccgggt
     2281 accagcacga gctgcttctt gagctcgtcg gcggcgtcct tcaggtcacg atacgtatag
     2341 ccgtcgccgg tcagcgccgc gtagatgccg tagacgtcgc cgaagtcgtc gatgacgcgt
     2401 ggcccctgca cgccgggcgg cagcttgccg tgcatgtcct gcagcttgcg gcgcagctcg
     2461 tcgtaaatgt ccgggaagtc gtcggccttg tactcgtcct tgaattcgat ggcgagatcg
     2521 gacatgcccg gtcgggagat ggacatcttg atgcgcttga gctggcccat aagctgcagc
     2581 gcttcctcga tgtggtaggt cacctcgtcc tgcacttcct gagccgtagc cccgggatac
     2641 agggtgatga tcttcgcctg cttga
//
LOCUS       DBNHOX010000152         1809 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_655306, whole genome shotgun sequence.
ACCESSION   DBNHOX010000152 DBNHOX010000000
VERSION     DBNHOX010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_655306"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="AC16UZ_03225"
     CDS             complement(<1..1041)
                     /locus_tag="AC16UZ_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR6245724.1"
                     /translation="MDNSNNVKLPDNVLPIIPARNMVVFPGEVSHISIGRESSIAAAD
                     LAAAEGRKLGLLLQHDPAVDAPAADDLHHVGTIVSIVRYVKAPNGTRHLICQGEERFR
                     AIGFASGYPFLASYADTLSDVAEEGPEIAARTDLLKERITEITSLLPETPMEFATALA
                     AVEQPGALADLVAGFLSIDAETKQTILETRNLKDRLDAVLAAATERLEVLRISQEIGA
                     KSKEKIDKQQREYVLREQLKTIQRELGEEGAGPELAEVAEAIDAARMPEEADKEARKE
                     LKRLQSMPESSAEYSMVRTYLDWLTALPWSELDSEEIDIARARAILDDDHYGLEKIKR
                     RILEYLAVRKLNP"
     gene            1314..>1809
                     /locus_tag="AC16UZ_03230"
     CDS             1314..>1809
                     /locus_tag="AC16UZ_03230"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6245725.1"
                     /translation="MKKLVRGGGACSYAVVLACAAFLPAGEAVAEETARASSQIIEEV
                     TVTARKREERVIDTPVAVSVMSEEDIERYNTRDLAQLTQRIPGLSIVNGQGGGAGGNI
                     TIRGIGKPPGTSDYGIDAPVSLVIDGMAFSRNHMIKTGFFDSEAVEVLKGPQALYFGK
                     NSPAG"
BASE COUNT          286 a          555 c          616 g          352 t
ORIGIN      
        1 cgggttcagc ttgcgcacgg cgaggtactc gaggatgcgg cgcttgatct tctccaggcc
       61 gtagtggtcg tcgtccagaa tggcccgtgc ccgggcgatg tcgatctcct ccgagtcgag
      121 ctctgaccac ggcagcgcgg tcagccagtc caggtacgtg cgcaccatgg agtactcggc
      181 cgaggactcc ggcatgctct gcaggcgctt gagctccttg cgcgcttcct tgtcagcctc
      241 ctccggcatc cgggctgcat cgatggcttc cgccacctcg gcgagctctg gcccggcacc
      301 ttcctcgccg agctcccgct gaatggtctt cagctgctcg cgcaggacgt actcgcgctg
      361 ctgcttgtcg atcttctcct tgctcttggc gccgatttcc tgggagatgc gcagcacctc
      421 caggcgctcg gtcgccgcgg cgaggacggc gtcgagccgg tctttgaggt tcctcgtctc
      481 caggatcgtc tgcttggttt ccgcatcgat gctgagaaag ccggccacca ggtcggccaa
      541 tgcgccgggc tgctccacgg cggccagcgc cgtggcgaac tccatggggg tctccggtag
      601 cagcgaggtg atttccgtga tcctttcctt gagcagatcg gtccgcgcgg cgatctcggg
      661 gccttcttcc gcgacgtcgg acagcgtgtc ggcataggag gccagaaacg ggtagccgct
      721 ggcgaagccg atggcgcgga agcgctcctc gccctggcag atgaggtgcc gggttccgtt
      781 cggggccttc acgtagcgca cgatgctgac gatagtgccg acgtggtgga gatcgtctgc
      841 cgcgggcgcg tccacggcgg gatcgtgctg cagcagcagg cccagcttcc ggccctccgc
      901 ggcggccaga tcagcggccg ctatcgatga ctcccggccg atggagatgt gggagacctc
      961 ccccgggaac accaccatgt tccgggcggg tatgatgggc agcacattgt ctggcagctt
     1021 gacgttgttc gagttgtcca tgctttgggg tggcgcatcg tgcgcggatg gtggagatgc
     1081 ttgggatatg tgggcgcgga gggggcgttt caagtaggtc cgaccggccc ccatcgagcc
     1141 cgtcgcggcg gcccggtgcc ccgggccgtg gcgcgcccat tggggcgggg ttttccgcta
     1201 gacagtcaaa ggtgactgtt ttagagtccg cagtgaggtc ctgcaggaga gagcaggaaa
     1261 ttttgggagt gcgccccggc cttcggggaa atcagcacag caaggggagt tctatgaaga
     1321 agctagttag aggcggtgga gcgtgcagct atgcggtcgt tttggcctgc gcggcgtttc
     1381 tgccggctgg cgaggccgtc gcagaggaga cggccagagc gtcgtctcag atcattgagg
     1441 aagtgaccgt caccgctcgg aagcgggaag agcgggtgat cgacacccct gtcgccgtgt
     1501 cggtgatgag cgaggaagac attgagcgtt acaacacccg cgaccttgcc cagctcaccc
     1561 agcgcattcc gggtctttcc atcgtcaacg gccagggtgg cggcgccggt ggcaacatca
     1621 ccatccgcgg tattggtaaa ccccccggaa cgagtgacta tggcatcgac gcacccgtgt
     1681 cactggtcat cgatgggatg gcgttttccc gcaaccacat gatcaagacc ggcttcttcg
     1741 acagcgaagc ggttgaggtt ctcaaaggtc cccaggcact ctacttcggc aagaacagcc
     1801 ccgccggcg
//
LOCUS       DBNHOX010000153        15538 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1233223, whole genome shotgun sequence.
ACCESSION   DBNHOX010000153 DBNHOX010000000
VERSION     DBNHOX010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 15538)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15538)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15538
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1233223"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="AC16UZ_03235"
     CDS             complement(<1..1132)
                     /locus_tag="AC16UZ_03235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6245726.1"
                     /translation="MTDNTLYGDGRRTAPSGPFAALARKALGRLRDGTIRLTEAGRTE
                     VFGCGAPEVHVEVRDPRFFRRLVMGGSVGAGESYMDADWSCDDLVSLIRIFARNRDAL
                     DVVDGAGSVLVRWANRSRHLLRRNTRRGSRRNIEAHYDLSNEFFETFLDGRMMYSSAV
                     FESPDATLEEASEAKLERLCRKLELGPEDHLLEIGSGWGGLAVYAAAQFGCRVTTVTI
                     SPAQLEATQARVRAAGLQGRVRAVLSDYRDLDGCYDKVVSVEMVEAVGHEFLDRYFES
                     LSGLTRPGGLIALQAITIADRRYRAALAEADFIKKHIFPGSFIPSVCVLVDAAARRTD
                     AVLVNLEDIGLDYARTLRAWRERLEARRGEIQSLGFDERFLRM"
     gene            complement(1129..1887)
                     /locus_tag="AC16UZ_03240"
     CDS             complement(1129..1887)
                     /locus_tag="AC16UZ_03240"
                     /inference="COORDINATES: protein motif:HMM:NF018770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HIR6245727.1"
                     /translation="MPPELLRDSVCEGWVYHRRAAPEHRFRYRVWMLWLDVARLGQGP
                     LRLLRRRLGLSPLGIAPKDFLDGSDDLLGAVRARLASHGYPAPARVFLLTQPRSWISG
                     FNPVSFYFCVGQAGLDCILAEINNTPWDERHTYVLDARGQSGEFSFSFPKAFHVSPFM
                     PMDLEYRWRFAIEPEAVKIAMGLFREGEETFNAGLRLSVVPMTRRRLLLGMARYPLQN
                     VATLARIYWQALRLYSKRSRFYPHPDRAEEAPAS"
     gene            complement(1877..3118)
                     /locus_tag="AC16UZ_03245"
     CDS             complement(1877..3118)
                     /locus_tag="AC16UZ_03245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6245728.1"
                     /translation="MRIAVVGAGVSGIAAAYRLQQEADVVLFEGQGRLGGHTDTHAVL
                     VGGATHAVDSGFIVFNPDNYPRFSSWLDELGVESQPSDMSFGVSNQATGVEYGSRGLG
                     ALFCQRRNLVSPRFLRMLADLRRFYRDAVDYPLDESLTLGELLDRHGYGAGFVEDHIG
                     PMCGALWSAAPDAALAIPARHVIAFMTHHRMLQLDGRPQWRVIKGGSSTYLQAFLARF
                     SGEVRLADPVQAVARTASGVDITSASGTARFDAVVLACHSDEALGLLADPSEAERDVL
                     GAIGYQPNRVVVHSDDRVMPEHRPAWSSWNALVGAERPERCQVSYWMNLLQSLPGREN
                     FFVTLNPQMPLKRVWSVREYAHPVFTRRARAAQRRKAEISGVRNTWFCGAYWGWGFHE
                     DGFVSGMEVAEGMLGSMADAA"
     gene            complement(3115..4014)
                     /locus_tag="AC16UZ_03250"
     CDS             complement(3115..4014)
                     /locus_tag="AC16UZ_03250"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006636 - unsaturated fatty acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HIR6245729.1"
                     /translation="MLATLSRQLDSERAMVRRGRGLDWLRIWPFIGLHLACLAVFWVG
                     VSPVAVVVAVACYLIRMFAITAFYHRYFAHKAFRTSRACQFVFAVLGAASTQRGPLWW
                     AAHHRRHHMHTDTADDPHRPQDGFLWSHMGWFLSGEHFQTDMARVRDWSVFPELVWLN
                     RFDTVVPILLGTAMFALGWGLERLAPGLGTSGWQMLIWGYFISTVALIHATLLVNSLA
                     HRSGRRCFDTRDESRNNWIVAVLTLGEGWHNNHHRFAGAARQGFYWWQLDVSFYLLKL
                     MSWLGLIWDLKPVPGSVLREGRS"
     gene            complement(4115..5722)
                     /locus_tag="AC16UZ_03255"
     CDS             complement(4115..5722)
                     /locus_tag="AC16UZ_03255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6245730.1"
                     /translation="MAKWQQPRSFWTWGYRSDEPSEEERRQAAARVSERLGRVVEPPP
                     IPRIGEIALRKPRLSVPAGLRGWVTTDHEERVVHTHGGHPLELLSAVRGEFDAPPDAV
                     AHPRNETELEATLAWCDEHGHAAIPFGGATSVVWGVRVPESADAAVTIDMDHFHQVLE
                     IDEVSRAARIQAGVLGPDLENTLRPLGLTLRHFPQSFPWSTVGGWVATRSGGHYATNH
                     THIDDFVESVRMLTPAGWWESRRLPGSGAGPSPDRMVMGSEGILGVISECWLRLQKRP
                     VYRATAGVVFDSWEAGAEAARQIVQAKLWPANLRLLDPAEAAQSAGLGGGRSLLIVGF
                     ESADLPQGDLMRDALAIARGAGGLVDDDAVRVDDGSGKATGREGSVGAWRSAFIGVNA
                     GLTSGLGLLADTFETAITWDRWPEFDASVRERVGRVLEEVLGPGARLSCRFTHVYPDG
                     PAPYYSFSGVAGLGAEHEQWRVIKDEATSAVVDAGGTVTHHHAVGRMHRAGWEDQHPP
                     LYAQALRAMKRTLDPNGILNPGVLIDP"
     gene            complement(5715..6989)
                     /locus_tag="AC16UZ_03260"
     CDS             complement(5715..6989)
                     /locus_tag="AC16UZ_03260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR6245731.1"
                     /translation="MAGERTLPVRLALAVALLVSSASAEPAAAGESTVIVLSWDGMRH
                     DYPDRGDFPGLARVISEGARAERLVTVFPSSTFPGHVSLATGTYPDRHGIVGNHFVDR
                     QRGVYRMDSDADWLLAEPIWIAAERQGVMAATYFWVGSESDWRGRGTRYRESPFDGSR
                     PEALKVDRILEWLQLPEGQRPRLIMSYWAGADRIGHRFGPDSERVTTQIAAQDLQLQR
                     LLQGLDLLDRWASTTLLIVSDHGMTASYRYTDLRGMLAAAGIGAYVTGSTVVHVYLDD
                     PDDHARAGELLGKIEGARAYPGDALPGWMRLSHAARTGDWLVVATPPLTFSRPAGWEG
                     AMVATLSMVGWTFGSHGYPPDHPEMPGMFLALGRGVPAGALLRAVHQVDLAATIAALL
                     GIEPPLESEGTPVPGLEIPVAARQAAKEDDDG"
     gene            complement(6979..7713)
                     /locus_tag="AC16UZ_03265"
     CDS             complement(6979..7713)
                     /locus_tag="AC16UZ_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013934.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR6245732.1"
                     /translation="MLEDVTRFGSKADARQWAWDALDERRLARFPFPPHGRIPNFAGA
                     AAAAMRIFAQAPWSEAGAIKVNPDSPQLPVRFLALQRGIRVYVPTPKLAAGFILLDPA
                     RIPPERFREAAALKTMLRWARPVALEDLPQLDAIVTGCAAATSSGKRAGKGAGYSDLE
                     FAILRELGHDPVPVATTVHDVQLVEDFPIAHNDLPLSLICTPTRTIRVQAPPAAPAGI
                     QWGELSAEQLAAMPVLESLRRLKHGG"
     gene            7773..9017
                     /locus_tag="AC16UZ_03270"
     CDS             7773..9017
                     /locus_tag="AC16UZ_03270"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6245733.1"
                     /translation="MTNFLLQRYPAFGFPRYRKYWLASLASVGATQLITLAQGWLVFE
                     LSGSALQLGILGAAVAAPNIAMTLIGGVFADRFDKRLILVWTSGAIATLLALLAWLDY
                     SGTVRVWHVLAVAAAVSLITGLDWPARVSIFPQLIDRPAFMSAVALNSIIWQATRMAM
                     PALGGIIIAASDTWVLFALGSGGFLTMMIVISGLHVTTSDARHGSPLEQIAEGVRFIV
                     NEELFKWLMLLTFIGMFFAGSYVQIMPVFADVLESGEAGYGYLLSAGGVGAVAGTLLV
                     GGVQEHARLGFVMLGGATLSAALTAVFALVAVGGWFIAALLATFFASVFTSVFMISSM
                     AVLQLVVPDDLRGRVMGIHAIGYSLIPLGGLFMGALAEAAGPALAVLIGCCVFGFAIA
                     GVAMTSTTIRGLDGRLIGHKQA"
     gene            9014..9232
                     /locus_tag="AC16UZ_03275"
     CDS             9014..9232
                     /locus_tag="AC16UZ_03275"
                     /inference="COORDINATES: protein motif:HMM:NF022596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HIR6245734.1"
                     /translation="MSEVGKWLVLAGALTVIVGLLLQTGWLGWFGRLPGDIRVERPGL
                     SFYFPITSMLIVSAILSVAAWILRRFLG"
     gene            complement(9236..9868)
                     /locus_tag="AC16UZ_03280"
     CDS             complement(9236..9868)
                     /locus_tag="AC16UZ_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245735.1"
                     /translation="MIYLVVNIVGYLLVALVAGVGVGWLLHGSLAGKASGERRGLGSV
                     GVDDLELAARADAQDEQLRKLAATIRQKEREVEALSRELERRDRRFNAADKLAKISRR
                     LQQEAEQLKEQVRVLGEQLEAAREEAQGSHGASAAMEEVLRSEILELEGRLRESSQEQ
                     DRLAKTLEQENRKVEELERQRDLQNSSLQVLHKQLELARKSREGAAEAGG"
     gene            complement(9865..10533)
                     /locus_tag="AC16UZ_03285"
     CDS             complement(9865..10533)
                     /locus_tag="AC16UZ_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR6245736.1"
                     /translation="MDLRFSQFEILTAAGAFFLCLAFICVFNEASDIQDDVGIRAQGE
                     AREHGLYWAGVEALGQRVRLTGAAPSDAQRLHAAAAVAALPGVTSVQNEIEVLGPDGA
                     CQRQMDSALAELGVTFKTGRAEIGDSSYPLIAELARTMGACATPIEIAVHTDSLGDAA
                     VNLKLSQRRADAVRKALVQRGVRPDRLVAAGYGETQPVASNARESGRRENRRVEFRVV
                     GGTA"
     gene            complement(10700..13351)
                     /gene="aceE"
                     /locus_tag="AC16UZ_03290"
     CDS             complement(10700..13351)
                     /gene="aceE"
                     /locus_tag="AC16UZ_03290"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014603513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR6245737.1"
                     /translation="MAERNPDDWLSEQQEWCDAIDDVLAFRGEDEVKELLRRLQAHVS
                     HRGMILTDAALNTPYKNTIDVRDQPAYPGNIELEMKIGDIICWNAVAMVLQAYDSGSG
                     VGGHIATYLSAATMMEVGFNHVFRGASESYGGDQVHFQAHTSPGVYARAFLEGRLSEQ
                     ALTNFRRELAPGGGLPSYPHPRRLPWFWQMPTASMGLSTPSAIYQARFAKYLESRGLK
                     ANNGGKIWTFIGDGESDEPEVLGTINIASRERLDNLVLVINCNLQRLDGPVRGNGKII
                     QELERAFRGADWHVIKVIWGGGWDAILARDEHGILQERMEQAVDGDYQMYTVSSGDVV
                     REHWVETSPELEELMKTLSDEEIRSIKRGGQDHQKIYAAFARAANVRGKPCVVLLKTV
                     KGDGLGPGTEGSNTVHQKKQLNEQERQELAKRLGIPLTREEISRAAFYRPAEDSAELV
                     YLRDRRHSLGGYLPAREVRCAALEPPSVELFAAMLKGSDRPVTTTAAVVRMLSRLLRD
                     PALGRYIVPIVPDEARTFGMDGLFPQAGIYSPAGQRYQPVDAGTIAPYREAEDGQILQ
                     EGICETGAMASFLAAGTAYANYGLPMIPFYIFYSIFGFQRVGDMIWAAGDMMCRGFLL
                     GGTSGRTTLNGEGVQHQDGHSHLVANTIPNLKSYDPAFAFEIALLVREGIRRMYQLQE
                     DVFYYLTLTNQPYPMPAMPEGVEDGVLKGMYCFSRAPGAVVNLFGSGALMTEVLRAES
                     LLAEHGIACNVWSVTSYNELARQALASERARLLGVGDGDERAYAEALLEGEEGIFVAA
                     SDYQKSLPMSISRWVPGPYTVLGTDGFGLSEDRRVLRGYFEVSAEWIAFAALSSLAQN
                     NRLERDVALGFAAKAGLALDKANPAEG"
     gene            complement(13489..14283)
                     /locus_tag="AC16UZ_03295"
     CDS             complement(13489..14283)
                     /locus_tag="AC16UZ_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017849031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR6245738.1"
                     /translation="MHLESDVAPEEAAHSLRLLTFNIQVGIRTTKYRHYVTKGWKHVL
                     PHEARAINIRHIADLVRDYDLVALQEIDGGSLRSGFINQVEYIADRARFPYWYTQCNR
                     DLGPFAQHGNGVLSRIAPVHMEDHKLPGAIPGRGAILMRFKLGESTVSLVVLHLSLGG
                     RSRQVQLEYVRELIDAEEHVVVMGDMNSHLAQLLFNSPLSETDLVPADQVHPTYPAWQ
                     PAFALDHVLVSPGLSIDDYEVLDCRLSDHRPIAVTLRLGEPVTIQQ"
     gene            complement(14425..15117)
                     /locus_tag="AC16UZ_03300"
     CDS             complement(14425..15117)
                     /locus_tag="AC16UZ_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HIR6245739.1"
                     /translation="MRDVLAFLLVVALGATGPAFAQEWQEGVHYQRLPVSVATADAAR
                     VEVVEVFSYACIHCKTFDPALEAWRENQPEDVGFRRVPAIFSAEWETLAKAFYTAQIL
                     DLGGAVHDSIFRAVHEEGRDLREPGAIAALFQQVAGVNPQQFAQVFNSFGVRSRIEQA
                     KAHARAYRVSGVPALVVDGTYRVDGRMAGSNAAMLEVVNHLVAKQRAARGIAAPEPAS
                     VEEEDAATSGAS"
     gene            complement(15249..>15538)
                     /locus_tag="AC16UZ_03305"
     CDS             complement(15249..>15538)
                     /locus_tag="AC16UZ_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6245740.1"
                     /translation="DDSIALAEQIYRGGIAKKGVAACAACHAPSGEGNASAGFPAVGG
                     QAGSYTIAQLTAYREGRRQTDEVYGGMMRGVAGGLTDGEIAALADYLQGLH"
BASE COUNT         2722 a         5252 c         5023 g         2541 t
ORIGIN      
        1 acatgcgcag aaaccgctcg tcgaagccca gcgactgaat ctccccgcgc cgggcttcca
       61 ggcgctcacg ccaggcgcgc agggtgcgcg cgtagtcgag gccgatgtcc tcgaggttga
      121 ccagcaccgc gtcggtccgg cgcgccgccg cgtccaccag cacgcagacc gagggaatga
      181 agctgccggg gaagatgtgt ttcttgatga agtctgcttc tgcgagcgct gcgcggtacc
      241 gccggtctgc aatggtgatt gcctgcaggg cgatgagccc acctggtcgg gtgagtccgg
      301 acagcgactc gaagtagcga tcgaggaact cgtgcccaac cgcctctacc atctcaacgg
      361 ataccacctt gtcgtaacag ccgtccagat cgcgatagtc cgacagaacg gccctgacgc
      421 ggccctggag cccggcggcc cgaacccgcg cttgggtggc ttcgagctga gcgggggaaa
      481 tcgtgaccgt ggtgacccga cagccgaact gcgctgccgc gtacacggcg agcccgcccc
      541 agcccgagcc gatctccaga aggtgatcct ccgggccgag ctcgagtttt cggcacagcc
      601 gctccagctt ggcttcgctc gcctcctcca gggttgcatc cggcgactcg aacacggccg
      661 acgagtacat catcctgccg tcgagaaaag tctcgaagaa ctcgttgctc aggtcatagt
      721 gcgcttcgat gttgcgccgg cttccccgtc gggtgttgcg cctcagcagg tgcctggagc
      781 ggttggccca tcgcacaaga acgctgccgg cgccatccac cacatccagg gcgtcgcggt
      841 tgcgggcaaa gattctgatc agagacacca gatcgtcgca ggaccagtcc gcgtccatat
      901 atgactcgcc ggccccgacg cttccgccca tgaccaggcg ccggaagaaa cgcggatcac
      961 ggacctccac gtgcacctcc ggggcgccgc aaccgaaaac ctcggtacgg cccgcctcgg
     1021 tcagccggat ggtgccatct cgcaacctgc ccagcgcctt gcgagccagc gcggcaaacg
     1081 ggcccgaggg cgcggttcgt cggccgtcgc catacaaggt gttgtcggtc atgatgccgg
     1141 cgcctcctca gcgcgatcgg ggtgcggata gaaacggctg cgcttggaat agagccgaag
     1201 ggcctgccag tagattcgtg ccagcgtcgc cacgttctgc agcggatagc gggccatgcc
     1261 caggagcagg cggcgccggg tcatgggcac taccgacagc ctcagcccgg cgttgaaggt
     1321 ctcttcccct tccctgaaca gacccatggc gatcttcacc gcttcgggct cgatggcgaa
     1381 ccgccaacgg tattcgagat ccatcggcat gaaaggcgag acgtgaaagg ccttgggaaa
     1441 agagaaggag aactcgcccg actggccgcg cgcgtccagc acgtaggtat ggcgttcatc
     1501 ccagggcgtg ttgttgatct cggcaagaat gcaatcaagc cctgcttggc ccacgcagaa
     1561 gtagaagctg accggattga acccggaaat ccagctgcgg ggctgggtga gcagaaacac
     1621 ccgggccggc gccgggtagc cgtgactggc caggcgcgcg cgaaccgcgc cgagaaggtc
     1681 gtcgctgccg tcgagaaagt ccttgggcgc tatcccgagc gggctcagtc ccagccggcg
     1741 ccgcagcagg cgcaagggcc cctggccgag gcgggcaaca tcgagccaga gcatccagac
     1801 gcggtaccgg aaacggtgtt ccggggcggc gcggcggtgg taaacccacc cctcacagac
     1861 gctatcgcgg aggagctcag gcggcatcgg ccatagaccc aagcatgcct tcggcaacct
     1921 ccatgccgct gacgaagccg tcctcgtgaa agccccagcc ccagtaagca ccgcaaaacc
     1981 aggtattgcg aacgccgctg atctcggcct tgcgacgctg ggctgcacgg gcacggcgag
     2041 tgaacaccgg gtgagcgtac tcgcgcacgc tccacacgcg cttgaggggc atctgcgggt
     2101 tcaacgtcac aaaaaagttt tcgcgcccgg gcaatgactg gagcaggttc atccagtagc
     2161 tcacctggca acgctcgggg cgctcggccc cgaccaaggc gttccagctc gaccacgccg
     2221 gccgatgctc gggcatgacc cggtcgtcgg agtgaacgac gacccggttc ggctggtagc
     2281 cgatggcgcc cagcacgtcc cgctccgcct cgctggggtc ggccagcaga cccagggcct
     2341 cgtcgctgtg gcaggccagc acgacggcat cgaagcgcgc ggttcccgac gccgaggtga
     2401 tgtccacgcc cgacgccgtc ctggctaccg cctgcaccgg gtcggccagc cgcacctcgc
     2461 cgctgaaacg ggccagaaac gcctgcagat aggtggacga cccgcccttg atcacccgcc
     2521 actgcgggcg accgtccagc tgcagcatgc ggtgatgcgt catgaaggcg atcacgtggc
     2581 gggccggtat cgccagagcc gcgtccggcg cagcggacca gagcgcaccg cacatgggcc
     2641 cgatatgatc ctcgacgaac ccggcgccgt agccgtgacg gtcgagcagc tcgccgaggg
     2701 tgagcgactc gtccagggga taatcgacgg cgtcacggta gaacctgcga agatcggcca
     2761 gcatgcgcag gaaccggggt gagaccaggt tgcgccgctg acagaacaaa gcgcccagtc
     2821 cgcggctgcc gtactcgacg ccggtagcct ggttcgacac gccgaaggac atgtccgacg
     2881 gctgagattc aacccccagc tcgtccagcc acgacgagaa acgcgggtaa ttgtccgggt
     2941 tgaacacgat gaatccggag tccacggcgt gggtggcacc gccgacgagc acggcgtggg
     3001 tgtcggtgtg accgcccagc cgcccctgcc cctcgaagag gacgacgtcg gcctcctgct
     3061 gcagccgata ggcggcggcg ataccggata cgcccgctcc caccaccgcg attctcacga
     3121 gcgcccctcc cggagcaccg agccagggac cggcttgaga tcccagatca agccgagcca
     3181 ggacatgagc ttcagcaggt agaaagagac gtcgagctgc caccagtaga atccctgtcg
     3241 ggcagcgccg gcaaagcgat gatgattgtt gtgccagccc tcgccgaggg tcagaacggc
     3301 gacgatccag ttgttgcggc tttcgtcccg cgtgtcgaag cacctgcggc ccgagcggtg
     3361 cgccagggag ttcaccagca gggtcgcgtg gatcaacgcg acggtagata tgaaataacc
     3421 ccagatcagc atctgccagc cgctggtgcc gaggccgggt gccagacgtt cgagccccca
     3481 tcccagagcg aacatggccg tgccgagcag gatgggcacc accgtgtcga accggttgag
     3541 ccagaccagc tccgggaaga cgctccagtc gcgaacccgg gccatgtcgg tctgaaaatg
     3601 ctcgccggag agaaaccagc ccatgtggga ccacaggaag ccgtcctgtg ggcggtgcgg
     3661 atcgtccgcg gtgtccgtgt gcatgtgatg tcggcggtgg tgcgccgccc accacagcgg
     3721 tccccgctgg gtggatgccg cgccgagcac ggcaaacacg aactggcagg cgcgcgaggt
     3781 gcgaaacgcc ttgtgcgcaa agtaacgatg gtagaaggcc gtgatcgcaa acatccggat
     3841 caggtagcag gccacggcga ccacgaccgc caccgggctc accccgaccc agaacacggc
     3901 caggcaggcc aggtgaaggc cgataaaggg ccagatgcgc agccagtcca agccgcggcc
     3961 gcggcgcacc atggcccgtt cggaatccaa ttgcctggaa agcgtggcca gcatcgcccc
     4021 caccctgaac gaagaggacg gccactatgc gcggccgcgc gtgaaagcca cgtcaacagg
     4081 ggagcagacg agcgagtcgc gggcgtgggc cgtcctaagg gtcgatgagc acgccgggat
     4141 tgaggatccc gttgggatcc agggtgcgct tcatcgctct gagcgcctgg gcatagagag
     4201 gtggatgctg atcctcccac cccgcacggt gcatgcgccc tacggcgtgg tgatgcgtca
     4261 ccgttccgcc ggcgtccacc accgcagacg tggcctcgtc cttgatgacc cgccattgct
     4321 cgtgctctgc cccgagcccg gcaacgccgg aaaagctgta atagggagcg gggccgtcgg
     4381 ggtagacgtg ggtgaagcgg caactgagcc tcgctcccgg cccgagcacc tcctcgagca
     4441 cccgacccac cctttcgcga accgacgcgt cgaactcggg ccagcgatcc caggtgatgg
     4501 ccgtctcgaa ggtgtccgcg agcagaccga gcccgctggt cagcccggcg ttgacgccga
     4561 tgaaggccga tcgccaggcc cccacggatc cttccctgcc ggtggctttc cccgacccgt
     4621 cgtccacgcg gaccgcatcg tcgtccacca ggccgcccgc tccccgtgcg atggccaacg
     4681 cgtcgcgcat cagatcgccc tgaggcaaat ccgcggactc gaaaccgacg atgagcaggg
     4741 accggccgcc gccgaggccc gcgctctggg cagcctcagc cggatccagc aggcgcaggt
     4801 tggcgggcca gagcttggcc tgcacgatct gcctggcggc ttccgcaccc gcctcccagc
     4861 tgtcgaacac cacgcccgcc gtggcccgat aaacgggccg tttctgcagc cgcagccagc
     4921 attccgagat gacgcctaga atgccttccg agcccatgac catgcgatcg gggctgggtc
     4981 cggcgccgct gccggggaga cgccgagatt cccaccatcc ggccggggtg agcatgcgaa
     5041 cgctctcgac gaagtcgtcg atgtgggtat ggttggtcgc gtagtgcccg ccggaacggg
     5101 tcgcgaccca gccaccaacg gtggaccagg gaaagctctg ggggaaatgc ctgagcgtca
     5161 ggcccagagg acggagggtg ttctccagat cgggccccag gacgcccgcc tggatgcggg
     5221 ccgcccggga cacttcgtct atctccagca cctgatggaa gtgatccatg tcgatggtca
     5281 ccgcggcatc cgcgctctcg ggcacccgca cgccccagac cactgaggtg gcgcccccaa
     5341 agggaatcgc ggcgtggccg tgctcgtcgc accaggcgag cgtcgcctcg agttctgttt
     5401 cgttgcgcgg atgggctacc gcatccggcg gcgcgtcgaa ctctcctcgc acggcagaga
     5461 gcagctcgag cgggtgaccg ccgtgggtgt gcaccacacg ctcctcatga tcggtggtca
     5521 cccaaccccg caggccggca ggaacggaca gccgcggctt gcgcagcgca atttccccga
     5581 tccgcgggat ggggggcggc tcgaccaccc ggccaagccg ttcggatacc cgagccgcgg
     5641 cctgacggcg ctcctcctcc gagggctcat cagaccgata gccccacgtc cagaagcttc
     5701 ggggttgctg ccacttagcc atcgtcatcc tccttggccg cctgccgggc ggcaacggga
     5761 atctccaggc ccggcaccgg cgtgccctcg gattcgagcg ggggctcgat gcccaggagc
     5821 gcggcgatgg tggcagccag atccacctga tgcaccgccc gtagaagtgc ccctgccgga
     5881 acgccacggc cgagggccag gaacatgccc ggcatctcgg gatggtccgg cgggtagccg
     5941 tggctgccga aagtccatcc caccatgctc agggtcgcca ccatggctcc ctcccaacca
     6001 gccggccggc tgaaggtcag gggaggagta gcgaccacca gccagtcgcc cgttctggcc
     6061 gcgtgggaca ggcgcatcca acccggcagc gcatccccag ggtaggcccg cgcgccctct
     6121 atcttgccga gcagttcccc tgcccgggca tggtcatccg gatcgtcgag gtagacgtgc
     6181 accaccgtgc tgcctgtgac gtaggcgccg atgccggcgg cggccagcat gccgcgcaga
     6241 tcggtgtaac ggtagctcgc ggtcatgccg tggtcgctaa cgatcaggag cgtggtagac
     6301 gcccagcgat ccagcaggtc gagaccctgg agcagccgct gcagctgcag atcctgagcg
     6361 gctatctggg tggtgacgcg ctcgctgtcg ggaccgaaac ggtggccgat ccgatcggcg
     6421 ccagcccagt agctcatgat gaggcgcggc cgctgcccct ccggaagctg cagccactcg
     6481 agaatccgat ccaccttcaa agcttcgggt cgggaaccgt cgaaggggct ctcgcggtaa
     6541 cgggtgcccc ggccgcgcca gtcgctctcc gagcccaccc agaagtaggt cgccgccatg
     6601 accccctggc gctcagccgc gatccagatg ggctcagcca gcagccagtc tgcatcggag
     6661 tccatcctgt agacgccgcg ctgccggtcg acgaagtgat tgccgacgat gccgtgccgg
     6721 tccgggtacg tgcccgtggc taaagagacg tgtcccggaa aagtagagga tggaaacacg
     6781 gtaacgagcc gctcggcccg ggcaccttcg ctgatcaccc gggccaggcc gggaaagtcg
     6841 cccctgtccg gatagtcgtg gcgcatgccg tcccaggaaa gcacgatcac cgtgctctcc
     6901 ccggcggcgg cgggctcggc cgaagccgag ctgacaagaa gcgccacggc cagtgccagg
     6961 cgcacgggaa gggtgcgctc acccgccatg cttgagcctg cgcaaggatt ccagaaccgg
     7021 catggccgcc agctgctcgg cggagagctc gccccattga atcccggcag gcgcggcggg
     7081 cggcgcctgg acgcggatcg tgcgcgtcgg cgtgcagatc aggcttaggg gcaggtcgtt
     7141 gtgggcaatg ggaaagtcct ctaccagctg cacgtcgtgg accgtcgtgg ccaccgggac
     7201 ggggtcgtgg ccgagctccc gcaggatggc gaactccagg tcgctgtacc cggcgccttt
     7261 ccccgcccgc ttgcccgacg aggtagccgc cgcgcacccg gtcacgatgg catccagctg
     7321 gggcaggtct tcgagggcta ccggccgcgc ccagcgcagc atggtcttga gcgccgctgc
     7381 ctcgcggaac cgctcggggg ggatccgcgc cggatccagc agaatgaatc ccgctgcgag
     7441 cttcggtgtc ggcacgtaaa cccggatccc acgctgcagc gccaggaacc gaacgggcag
     7501 ctggggcgag tcgggattga ccttgatagc ccccgcctcg gaccaggggg cttgcgcaaa
     7561 aatccgcatc gcggctgccg ccgcgccggc gaagttcgga atccgcccat ggggtgggaa
     7621 cggaaatctg gccagacgac gctcgtcgag ggcatcccac gcccattgac gcgcgtccgc
     7681 cttggagccg aacctggtga cgtcttctag caaggcgagt ggtgccagcc aatacggtca
     7741 cggcatacta cacggccgct cagagagatg gcatgaccaa cttccttctg caacgctacc
     7801 cggctttcgg cttcccgcgc taccgaaagt actggctggc gtctcttgcc tccgtgggcg
     7861 cgacccagct gatcaccctg gcccagggtt ggctggtatt cgagctatca gggtcggcgc
     7921 tgcagctggg aattctgggc gccgccgtcg ccgcgccgaa catcgcgatg acgctgatcg
     7981 gcggcgtgtt cgccgaccgc ttcgacaagc gtctcattct ggtctggaca tcgggggcca
     8041 tcgccacgct actcgcgctg ctggcctggc tggactacag cggtaccgtg cgcgtgtggc
     8101 acgtcctcgc cgtggcggcc gcagtctctc tcatcaccgg gctcgactgg cccgccaggg
     8161 tctccatctt tccacagctc atcgaccggc cggctttcat gagcgccgta gcgctcaact
     8221 ccatcatctg gcaggccacg cgcatggcca tgccggcact gggcgggatc atcatagcgg
     8281 ccagcgacac ctgggtgctg ttcgccctgg gcagcggcgg ctttctgacc atgatgatcg
     8341 tcatcagcgg cctgcacgtg accaccagcg acgcgcgcca cggctcgccg ctggagcaga
     8401 tcgccgaggg cgtgcgcttc atcgtcaacg aggagctgtt caagtggctg atgctgctca
     8461 cgttcatcgg catgtttttc gcgggcagct acgtgcagat catgcccgtg ttcgcagacg
     8521 tcctggagtc cggtgaggcc ggctacggtt atctgctctc ggccggaggc gtgggcgcgg
     8581 ttgccggcac gctgctggtc gggggggtgc aggagcatgc gaggctcggg ttcgtcatgc
     8641 ttggcggcgc aaccctgtcc gccgccctaa ccgccgtgtt cgctctggtc gcagtgggtg
     8701 gttggttcat cgccgcgctg ctggcgacgt ttttcgcatc ggtcttcacc tcagtcttca
     8761 tgatcagctc catggccgtc ctgcagctcg tcgttcccga cgacctgcgc ggccgggtca
     8821 tgggcatcca cgcgattggc tacagcctga taccgttggg aggcttgttc atgggcgctc
     8881 tcgccgaggc cgctgggccg gcgctggcgg tgctgatcgg ctgttgcgtg ttcggcttcg
     8941 ccatcgccgg cgtagccatg accagcacga ccattcgcgg tctcgatggt cgcctgatcg
     9001 ggcacaagca agcatgagcg aggtgggcaa atggctggtg ctcgccggag cgttaaccgt
     9061 gatcgtcggg ttgctgctgc agacggggtg gctcggatgg ttcggcaggc tgccgggaga
     9121 catccgggtg gagcgcccgg ggctctcctt ttactttccc atcacctcga tgctcatcgt
     9181 ctcggccatc ctctcggtgg ccgcctggat cctccgacgc ttcctcggct gagcctcaac
     9241 caccggcctc ggcagcgccc tccctgctct tccgggccag ctccagctgc ttgtgcagaa
     9301 cctgcagaga gctgttctga agatcacgct gccgctcgag ctcctcgacc ttgcggtttt
     9361 cctgctccag cgttttggcg agccggtcct gctcctgaga agactcccgg agccggccct
     9421 cgagctcgag gatctcgctg cgcagaactt cctccatggc agccgacgcg ccgtgggatc
     9481 cctgggcctc ctcccgcgcg gcttcgagct gctcgccgag aacgcggacc tgctccttga
     9541 gctgctcggc ttcctgttga agacgccggg atatctttgc cagcttgtcg gcagcgttga
     9601 agcggcgatc ccgcctttcg agctcgcggc tcaacgcctc gacctctcgc tccttctgcc
     9661 tgatcgtggc ggccagcttc ctcagctgct cgtcctgggc gtcggcgcga gccgcgagct
     9721 cgagatcgtc gacgcccacg gaaccgagcc ctctgcgctc gccggaagcc ttgcctgcga
     9781 gggatccgtg cagcagccac cccacaccga cgccggcgac cagggcaacc agcagatagc
     9841 cgacgatgtt gacgacgaga tagatcacgc ggtgcctccg accacccgga attcaacccg
     9901 gcgattttcc cgacgtcccg attcgcgggc attggaagcg acgggctggg tctcgccgta
     9961 gccggcagct acgagacggt ccgggcgaac gcctctttgg accagagcct tgcgtaccgc
    10021 gtccgctcgc cgctgactca gcttgaggtt gacggcggca tcgccgaggg aatccgtgtg
    10081 cacggcaatc tcgatgggcg tcgcgcatgc gcccatggtc cgggcaagct cggcaatcaa
    10141 cgggtagctg gagtcgccga tctcggcgcg accggtcttg aaggtcacgc caagctccgc
    10201 cagcgccgag tccatctgcc tctgacacgc gccgtcgggg ccgagcacct cgatctcgtt
    10261 ctggaccgaa gtgacacctg ggagcgcggc gaccgctgct gccgcgtgaa gcctttgggc
    10321 atcgcttggc gcggcacccg tcagcctgac ccgctggccc aaggcctcca cacccgccca
    10381 gtaaaggccg tgctcgcgcg cctcgccctg ggcgcgtatg ccgacgtcgt cctggatatc
    10441 tgatgcctcg ttgaacacgc aaatgaacgc caggcaaaga aagaatgcgc cggcggctgt
    10501 gaggatctcg aactgtgaga aacgaaggtc cattttcctt acttacagga ttcgagccga
    10561 gttggtccct tgggggattt tgaagcgatg gcggctcgtt ttacgtgaac cgattctcaa
    10621 cttacccgac ccgcgcgggc aaaggcaacg ccaatccggg gccccatcgg cgcatgcccc
    10681 agagcacatc ccgcgcccct cagccctcag cggggttcgc cttgtccagg gcaagccccg
    10741 ccttggccgc aaagcccagc gccacgtcac gctcgagccg gttgttctgg gccagggacg
    10801 acaacgcggc gaacgcgatc cactcggcgg aaacttcgaa gtagccgcgc agaacccggc
    10861 gatcctccga cagcccgaag ccgtccgtgc ccagcacggt ataggggccc ggcacccagc
    10921 gagaaatgct catgggaagg gacttctggt agtccgaggc ggccacgaat atcccttctt
    10981 ccccctctag cagcgcttcg gcgtaggccc gctcatcgcc gtcgccgaca ccgagcaggc
    11041 gggctcgctc cgaggcgagc gcctgccggg ccagctcgtt gtagctcgta acgctccaga
    11101 cgttacacgc aatgccatgc tcggcaagaa ggctctccgc ccgcaacacc tcggtcatca
    11161 gcgcgccgct gccgaacagg ttgaccaccg cccccggagc acggctgaag cagtacatgc
    11221 ctttgagaac gccgtcctcc acgccttcgg gcatggccgg catggggtag ggctggttgg
    11281 tcagggtgag atagtagaag acgtcttcct gcagctgata catccgcctg atgccctcac
    11341 gaaccagcaa ggctatctcg aaggcgaacg ccgggtcgta gctcttcagg ttgggaatcg
    11401 tgttggcaac cagatgggag tgcccgtcct gatgctgcac cccctcgccg ttcagggtgg
    11461 tacgccctga agtgccgccg aggagaaaac ccctgcacat catgtcgccc gcggcccaga
    11521 tcatgtcgcc gacacgctga aaaccgaaga tcgagtagaa gatgtagaac ggaatcatcg
    11581 gcaggccata attcgcgtag gccgtgcccg cggccaggaa agacgccatg gcgcccgttt
    11641 cgcagattcc ttcctggaga atctgaccat cctcggcctc ccgatacggc gcgatggtgc
    11701 ccgcatccac cggctggtag cgctgaccgg ccggcgagta gatgcccgcc tggggaaaaa
    11761 ggccgtccat cccgaaggtg cgcgcctcgt cgggaacgat gggcacgatg tatcggccga
    11821 gagccgggtc acgcagcagg cgggacagca tacggaccac cgccgccgtg gtggtcaccg
    11881 gcctgtccga tcctttgagc atggcggcaa aaagctccac cgacgggggc tccagcgccg
    11941 cgcagcgcac ttcccgggcc ggcaggtaac cgcccaggct atggcgccgg tcgcgcaggt
    12001 agaccagctc tgcagagtcc tcggccgggc gatagaaagc cgctcgcgat atctcctccc
    12061 gggtgagcgg aatgccaagc ctcttcgcga gctcctgtcg ctcctgctcg ttgagctgct
    12121 tcttctggtg caccgtgttg ctgccttcgg tgcctggccc gagaccgtcg cccttcaccg
    12181 tcttgagaag caccacgcac ggcttgccgc gaacgttggc cgcccgggca aacgcggcat
    12241 agatcttctg gtgatcctgg ccgccacgct tgatggaccg gatctcctcg tcggagagcg
    12301 tcttcatcag ctcctcgagc tcgggggatg tttccaccca gtgctcgcgg accacgtcgc
    12361 cgctggacac cgtgtacatc tgatagtcgc catccacggc ctgctccata cgctcctgga
    12421 gaatgccgtg ctcgtcccgg gcaagaatgg catcccagcc gccgccccag atcaccttga
    12481 tcacgtgcca gtcggcgccg cgaaaggcgc gctcgagctc ctgaatgatt tttccgttgc
    12541 ccctgaccgg gccatcgagc cgctgcagat tgcaattgat caccagaacc aggttgtcca
    12601 gccgctcccg gctggcgatg ttgatggtgc cgagcacctc cggctcgtcg gactctccgt
    12661 ccccgatgaa cgtccagatc ttgccgccgt tgttggcctt gagcccgcgg ctttccagat
    12721 atttggcgaa ccgcgcctgg tagatggcgc tgggcgtaga gagccccatg ctggccgtgg
    12781 gcatctgcca gaaccacggc aggcggcgcg gatgagggta ggagggcaga ccaccaccgg
    12841 gcgccagctc gcggcggaag ttggtcaacg cctgctcgga aagacgccct tccagaaacg
    12901 ccctggcata gacgccgggc gaggtgtggg cctgaaagtg cacctgatcg ccgccgtagg
    12961 attcggacgc gcctcgaaag acgtgattga aacccacttc catcatcgtc gcggcggaca
    13021 ggtaggtggc gatgtgacca ccgacccccg agccgctgtc gtaggcctgc agcaccatgg
    13081 cgaccgcgtt ccagcagatg atgtcgccga tcttcatctc gagctcgatg ttgcccgggt
    13141 aggcgggctg atcgcgcacg tcgatggtgt tcttgtacgg ggtgttgagc gcggcgtcgg
    13201 tcagaatcat gccgcgatga ctgacatggg cctgcagccg ccgaagcagc tccttgacct
    13261 cgtcctcacc cctgaaggcg agcacgtcgt caatggcgtc gcaccattcc tgctgctccg
    13321 acagccaatc gtcggggtta cgctccgcca tctccgtcac cgtacccaat gtcttaggca
    13381 agcggggaag ctacgccatg gggcaagcgc ggtaaaccgc tccgagaaac ggtcgctgag
    13441 ccgggaacgg gatcttgggc gagaatgcgg ccggacgagc acgccagcct actgctgaat
    13501 cgtaacgggc tccccgaggc gcagggtgac cgcgatcggt cgatgatcgg agagccggca
    13561 gtccagaacc tcgtagtcgt cgatcgacag ccccggcgag accagcacgt gatccagagc
    13621 aaacgcaggc tgccaggcgg gataagtggg gtgcacctga tccgccggca ccagatccgt
    13681 ttccgaaagc ggcgagttga agagcagctg ggcaagatga ctgttcatgt ctcccatcac
    13741 caccacgtgc tcttcggcgt cgatcaactc gcgcacatac tcgagctgca cctggcggga
    13801 acgcccgccc aaagagaggt gcagaaccac cagcgagacc gtcgactcgc cgagcttgaa
    13861 gcgcatcagg atcgcgccgc gtcccggaat ggcgcccggc agcttgtggt cttccatgtg
    13921 cacgggcgcg attcgcgaca gaaccccgtt gccgtgctgg gcgaacggac ccaggtcacg
    13981 gttgcactgg gtgtaccagt aaggaaaacg cgcgcgatcg gcaatgtact ccacctgatt
    14041 gatgaagccg ctgcgaaggc tgccgccgtc gatttcctga agcgcgacca gatcgtagtc
    14101 gcgcaccaga tcggcgatgt ggcggatatt gatggcccgc gcttcgtgag gcagcacgtg
    14161 cttccagccc ttggtgacgt agtggcggta cttggtggtc cgaataccca cctgaatgtt
    14221 gaaggtgagg agcctgagcg aatgcgccgc ctcctcggga gccacatcgg actccaagtg
    14281 catgttcacg accggggtga cagccgtact acggttctcg ccgcgcctag accaagccaa
    14341 catggcggac tgctccatcg acttggtcat gagtctagcc ttgcgctgcg gattggactg
    14401 cgtactaact aacatttcgt tcgatcacga ggccccagaa gtggccgcgt cctcttcctc
    14461 aacggacgcc ggctccgggg cggcgatgcc acgcgccgct cgctgctttg cgacaaggtg
    14521 gttgaccact tccagcatgg cggcgttgct gcccgccatc cgaccgtcca cgcgataggt
    14581 gccatccacc accagggcgg ggaccccgga gacgcgataa gcccgggcat gggccttggc
    14641 ctgctcgatc cggctgcgaa ccccgaagga gttgaagacc tgagcgaact gctgggggtt
    14701 cacgccggcg acttgctgga acagggcggc aatggcgcca ggttcgcgca ggtcccggcc
    14761 ctcttcgtgc accgcccgaa agatggaatc gtgcacggcg ccgccgagat ccaggatctg
    14821 ggcagtgtag aacgctttgg cgagggtttc ccactcggcg ctgaaaatcg cgggaacgcg
    14881 cctgaaaccc acgtcctcgg gctggttctc tcgccaggct tccaacgccg ggtcgaaggt
    14941 cttgcagtgt atgcaggcgt aggagaatac ctccaccacc tctaccctgg cggcgtctgc
    15001 cgtggccacg ctcaccggca gccgctgata gtgaacgccc tcttgccatt cctgggcaaa
    15061 ggcggggccc gttgccccga gggcaaccac tagcaggaaa gcgagcacgt ctctcatttc
    15121 tacctctctg gaggactctg ttcggccccg accatgaccc tgcggcgacg ggtaggcctc
    15181 gtccccgcca tgcgcgattc gaaccgaggg cgccttggga accactcgtt ggacaacgtc
    15241 cggttcgctt agtgcagccc ctgaagatag tcagccaggg ccgcgatctc accatcggtc
    15301 aggccgccgg ccacgccacg catcatgccg ccgtatacct cgtcggtctg tcggcgacct
    15361 tcccgatagg ccgtgagctg ggcgatggtg taactacccg cctgcccgcc aacggcggga
    15421 aagccggcag aggcgttgcc ttcgccgctc ggcgcatggc aagcggcgca ggccgccaca
    15481 cctttcttgg cgatcccgcc gcgatagatc tgctcggcga gcgcaatgga gtcgtcgt
//
LOCUS       DBNHOX010000154         1682 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_12133, whole genome shotgun sequence.
ACCESSION   DBNHOX010000154 DBNHOX010000000
VERSION     DBNHOX010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_12133"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..590
                     /locus_tag="AC16UZ_03310"
     CDS             162..590
                     /locus_tag="AC16UZ_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6245741.1"
                     /translation="MKTIAEHNDWRGKANLDDGEWSTFVNPETSPDFFDHMTDVVKVK
                     FMGESWETGPWVVPNKFPPNTRADRHAHAADTIYVIMRGSMTFNDGTGWYEPGDVRWV
                     RADHTYGPEESGPDGCEFILISMGPIDVQWETGRTFHPDT"
     gene            complement(654..836)
                     /locus_tag="AC16UZ_03315"
     CDS             complement(654..836)
                     /locus_tag="AC16UZ_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245742.1"
                     /translation="MFDKAEFAARVNGLRVQRDELKAEIEALDMADGDARAQEVVWRE
                     RQIEQLQRIIDALENS"
     gene            complement(836..1240)
                     /locus_tag="AC16UZ_03320"
     CDS             complement(836..1240)
                     /locus_tag="AC16UZ_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HIR6245743.1"
                     /translation="MLRSVGSFHPGDFIRVGENFGRVTEKGLVHTEIQSEDRDLITLP
                     NLYLMNQPVGVIRTSGTLISAEVTLGYDVHRHRLRDLLKSAAEAAELSGPFVQILELG
                     DHAIRYRVYGFLEDVANLVTKCRSRKTPTPSN"
     gene            complement(1353..>1682)
                     /locus_tag="AC16UZ_03325"
     CDS             complement(1353..>1682)
                     /locus_tag="AC16UZ_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HIR6245744.1"
                     /translation="AIGETERRRAKQTAFNEAHGITPRTIEKQIADVMEGARAPAPGT
                     VRRKGAGPAHGSAERIEIPEDPKAFGRLLSELEERMLEHARNLEFEEAAQLRDELKKI
                     REDRLLA"
BASE COUNT          313 a          548 c          500 g          321 t
ORIGIN      
        1 agagatcgag cacggccacc cgcatcggtc gtgaccgtgc tctctgcccc gccgtccggg
       61 aagtcttgcc aaccccgcgc ccacgtctga agatacgagg agcagcttag ctaccgggac
      121 ggggcccaca ccaggagggc cgccgagacg atggggagag catgaaaacc atagcggagc
      181 acaacgactg gcggggtaaa gccaacctcg acgacggcga gtggtcgacg ttcgtgaatc
      241 cggagacgtc gccggacttc ttcgatcaca tgaccgacgt ggtgaaggtc aagttcatgg
      301 gcgaatcctg ggagaccggc ccctgggtgg ttcccaacaa gttcccgccg aacacccgcg
      361 ccgaccgcca cgcccatgcg gcagacacca tctacgtgat catgcggggg agcatgacgt
      421 tcaacgacgg caccggatgg tacgagcccg gagacgtccg atgggtgcga gctgatcaca
      481 cctacggacc cgaggagtca ggaccggacg gttgcgagtt catactcata tccatggggc
      541 ccatcgacgt ccagtgggaa acgggacgca cgtttcatcc agacacttaa agcccctccg
      601 gcgcacgaac gcgggggagg ttacgacttc aatcgggaac tcaccggtat gcctcaggaa
      661 ttttccagcg catcgatgat gcgctgcagc tgctctatct gccgctctcg ccagacaact
      721 tcctgagccc gagcgtcgcc gtcagccatg tcgagggctt cgatttccgc cttcagctcg
      781 tcgcgctgaa ccctgagccc gttgacccgg gcggcaaatt cagccttgtc gaacatcagt
      841 tgctcggcgt gggagtcttc ctgctcctgc acttcgtgac gaggttcgcg acgtcctcca
      901 ggaagccata aacccgatac cttatcgcgt ggtcgccaag ctcaaggatc tgcacgaacg
      961 ggccggagag ctcggccgct tctgctgcgg atttcagcag gtcccgcagc ctgtgccggt
     1021 gaacgtcgta gcccagggtc acctccgcgg atatcagggt ccccgaagtg cggatcaccc
     1081 cgacgggctg attcattaga tacaagttgg gcagcgttat caggtcccgg tcttcgctct
     1141 ggatctccgt gtgcacaagt cccttttcgg tgacccgtcc gaaattctcg ccaacgcgaa
     1201 tgaagtctcc tggatgaaaa ctcccgaccg accgcagcat gaaccccgcc ggtcgagatc
     1261 aatgcccggc gtgacggccc cttccgaaaa cccacggatg ttggagttct gaatccatcc
     1321 tgccgattct agtctgctcg aacggcgtcg tctcaggcga gcagccggtc ttcgcgaatc
     1381 tttttcagct cgtcgcgcag ctgcgcggcc tcctcgaatt ccaggttgcg agcgtgctcg
     1441 agcattcgct cctccagctc cgagagcagc ctgccaaacg ctttcggatc ctctggaatc
     1501 tcgatgcgct cggccgatcc gtgtgcaggc ccggcgcctt tccggcgcac cgtgccgggc
     1561 gcgggagctc tggcaccctc catcacgtcg gcgatctgct tttcgatggt gcgtggcgtg
     1621 atgccgtgag cctcgttgaa cgccgtctgc ttggcacgcc ggcgttcggt ctctccgatg
     1681 gc
//
LOCUS       DBNHOX010000155         2507 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_591370, whole genome shotgun sequence.
ACCESSION   DBNHOX010000155 DBNHOX010000000
VERSION     DBNHOX010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_591370"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="AC16UZ_03330"
     CDS             <1..406
                     /locus_tag="AC16UZ_03330"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR6245745.1"
                     /translation="FVSGRYSREHRWTFDGGAVVQASPTPAVVPEPFSNPACVDPEEA
                     FVAAVASCHMLTFLHIAGRAGFSVIGYRDEAVGTMTKNAQGAYWISEITLNPAIEYAG
                     NAPTEAELDDLHDQAHEHCFIANSIRTEVLVG"
     gene            493..1482
                     /locus_tag="AC16UZ_03335"
     CDS             493..1482
                     /locus_tag="AC16UZ_03335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6245746.1"
                     /translation="MASRPEVEVEPGNFELVEEPVSGLEPGTLLLKTRYLTVNPPMRM
                     ALVSGGIAGKPIPIGRTMYGSGLAEVVESRNPAFAAGDLVMGDLGWQEYVVSDGARRR
                     GVRKVAVPERLSPTTLMHVLGGSGHTAYFGMIEFCKPRVGDTLVVSTAAGSVGALVCQ
                     LGRLQGCRVIGIAGSDEKCEWVVDELGAEACINYRTEDVAARLAETCPNGIDVYFDNV
                     GGETLDAVLGQIAMGARVVLCGGTSQYNNDLDWYGPKNYFNLVYKQAEMSGYFVSNFA
                     HRFDESLARLTTLVRDGRLKYREDVLEGIESVPSALIRILRGENFGIPLIKVS"
     gene            1484..>2507
                     /locus_tag="AC16UZ_03340"
     CDS             1484..>2507
                     /locus_tag="AC16UZ_03340"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6245747.1"
                     /translation="MSTVMRGANEPPTCHGGLENLDEEHDYWIDDIDGTIPADLTGTF
                     MRNGPGRQKIGGKPFGHWFDGDGMISQFTFTDSKAHFRNRYVRTPKYVEETEAQEILY
                     RGFGTQIPGGWTKNIFKWPANPANTSIIHHGGRLLALNEGGRPWELEPGSLETVGEFD
                     YDGALAGKAFSAHGKVHPQTGDYFNFGAGTTGTVRKSPCLNMYRINPQGRMAKTAQLP
                     LQTFPFCHDFALAGRHAVYFVNSIVFGGMGGFFLGRHSMSDGVRFDDDIPMRIIVVDL
                     DTLEVVREFETDPGAIIHFGNAFESGDEVVVDAMFQDNFEVNATLADVFNPSGRFGGG
                     YYNRYRL"
BASE COUNT          467 a          765 c          825 g          450 t
ORIGIN      
        1 cttcgtcagc ggccgctatt cccgcgagca ccgctggacg tttgatggcg gtgcggtggt
       61 tcaggcatcg cccacgccgg cggtggtgcc ggagccgttt tcgaatcctg cctgcgtaga
      121 tccggaagag gccttcgtcg cggcggtggc cagctgccac atgcttacct tcctgcacat
      181 cgccggccgt gcgggatttt cggtgatcgg ctatcgcgac gaggcggtgg gcaccatgac
      241 caagaacgcc cagggtgcgt actggatcag cgagatcacg ctcaatccgg cgatcgaata
      301 cgccggcaac gcgcctacgg aagcggaact ggacgacctg cacgatcagg cgcacgagca
      361 ttgcttcata gcgaactcca tccgcaccga ggtgctcgtc ggctgacgtg tgattagacg
      421 gctaccggta cgatgggcac ggacgagtag agggggtaga acatcagcgg cctgatcaac
      481 agaaagtggg tggtggcgtc tcggccggaa gtcgaggtgg aaccgggcaa cttcgaactc
      541 gtggaggaac cggtttctgg gttggagccc ggcaccctgc tgctcaagac ccgatacctc
      601 accgtgaacc cgcccatgcg catggcgttg gtttccggcg gtatagccgg gaagcccatc
      661 cccattggcc ggaccatgta cggcagcggc ctggccgagg tggtggagtc tcgcaacccg
      721 gcctttgccg cgggagacct ggtcatggga gacctcggct ggcaggagta cgtggtctct
      781 gacggcgcgc gccggcgtgg ggtgcggaag gtcgcggtgc ctgaacggct gtcacctact
      841 accctcatgc acgtgctcgg gggaagcggg cacacggcct acttcgggat gatcgagttc
      901 tgcaaacccc gggtgggcga caccttagtg gtctcaaccg cggcgggcag cgtgggcgcg
      961 ctcgtctgcc agctcggccg cctgcagggc tgtcgggtga tcggcattgc cggcagcgac
     1021 gagaagtgcg agtgggtggt cgacgagctc ggtgccgagg cctgcatcaa ctaccgcacg
     1081 gaagacgttg ccgcgcgtct ggccgaaacc tgcccgaacg gcatagacgt ctacttcgac
     1141 aacgtcgggg gcgagacgct cgatgcggtg ctcgggcaga tcgccatggg cgcccgcgtg
     1201 gtcctctgtg gcggcacgtc gcagtacaac aacgacctcg actggtatgg ccccaagaac
     1261 tatttcaacc tggtttacaa gcaggcggag atgtccggct acttcgtctc caacttcgcg
     1321 caccgtttcg atgagtctct cgcccggctg accaccctcg tccgcgacgg gcgtctgaag
     1381 tatcgcgagg acgttctcga gggcatcgag agcgttccgt cggcgctcat ccggattctg
     1441 cgcggcgaga atttcggcat cccactgatt aaggtttcat gacatgagca cggtaatgcg
     1501 aggcgccaac gagccgccta cctgtcacgg cggcctggag aatctggacg aggagcacga
     1561 ctactggata gacgacatcg acggaacgat tcctgcggac ctcaccggca cgttcatgcg
     1621 caatggtccg gggcgtcaga agatcggcgg caagcccttt ggtcactggt tcgacggtga
     1681 cggcatgatc agccagttca cgtttaccga ttccaaagcc cacttccgca accgatacgt
     1741 cagaacgccc aagtacgtcg aggagaccga agcgcaggag atcctctatc ggggattcgg
     1801 cacgcagatc cccggaggct ggaccaagaa catcttcaag tggccggcca accccgcgaa
     1861 caccagcatc atccaccacg gtggccggct gctggcgctg aacgagggcg gtcgcccgtg
     1921 ggagctcgag ccgggctctt tagagacggt gggcgagttc gactacgacg gcgcgctcgc
     1981 gggcaaggcg ttcagcgccc acggcaaggt gcatccgcag acgggcgact acttcaactt
     2041 cggcgccggg accacgggca ccgtgaggaa atcaccgtgc ctgaatatgt accgcatcaa
     2101 tccccagggt cgcatggcaa agacggcgca gcttccgctg cagacgtttc cgttctgcca
     2161 cgatttcgcc ctggccgggc gccacgccgt gtacttcgtc aactccatcg tctttggcgg
     2221 catgggcggt ttcttcctgg gaagacacag catgtcggac ggcgtgcggt tcgatgacga
     2281 catccccatg cgcatcatcg tcgttgatct cgacaccctg gaggtggtgc gcgagttcga
     2341 gacggatccg ggcgcgatca ttcacttcgg caacgcgttc gaaagcggtg acgaggtggt
     2401 cgtggacgcc atgttccagg ataacttcga ggtcaacgcg acgctggcag atgtgttcaa
     2461 cccgtcggga aggttcggcg gcgggtacta caaccgctac cgcctga
//
LOCUS       DBNHOX010000156         1906 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_12339, whole genome shotgun sequence.
ACCESSION   DBNHOX010000156 DBNHOX010000000
VERSION     DBNHOX010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_12339"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="AC16UZ_03345"
     CDS             <1..341
                     /locus_tag="AC16UZ_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245748.1"
                     /translation="SGKLPTGMLQALECHVDLAGPLRRSVIGCLARLGTGGPQQSPDR
                     LQVSLTELPLTGDHPGPVLGLHARTAAGDVIGSKRLQAPILECSLGPLLLEFALEWLW
                     IRNDKPGFAR"
     gene            complement(310..1374)
                     /gene="hemE"
                     /locus_tag="AC16UZ_03350"
     CDS             complement(310..1374)
                     /gene="hemE"
                     /locus_tag="AC16UZ_03350"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016979196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIR6245749.1"
                     /translation="MTALKNDLFLRALRHEPVERTPVWIMRQAGRYLPEYLKTRAEAG
                     SFMNLCQSPQLACEVTLQPLRRFALDAAIIFSDILTIPDAMGLGLSFAAGEGPRFERP
                     VRSASAVRKLPRPDVGESLAYVMDAISLTRRELGGKVPLIGFSGSPWTLATYMVEGGS
                     SKTFGTVKKMIYQEPAVAHELLGKLADTVTDYLNAQIASGAQAVQIFDTWGGALSAPA
                     YREFSLAYMARIVAGLQRESEGRPVPVILFSKGCNTQLEALAETGCDALGVDWTITLA
                     EARQRVGGRVALQGNLDPAVLLADPDTIRREVAGTLASFGHGPGHVFNLGHGITPDVN
                     PDHLAALVDAVSEQSPAYHS"
     gene            complement(1371..>1906)
                     /locus_tag="AC16UZ_03355"
     CDS             complement(1371..>1906)
                     /locus_tag="AC16UZ_03355"
                     /inference="COORDINATES: protein motif:HMM:NF049783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HIR6245750.1"
                     /translation="FFEWLEQNAGPVNARDAEALEYLIHRSVLNKAEIVARDEKESGV
                     RALLNFGHSFGHALETETGYQRFLHGEAVAIGMVVAASLSESRGLCSKGTAQRLATLL
                     ERCGLPVRIPPDRSIERLAAALELDKKAVASGLRLVLLDAVGRARIDDRCGHDEIKEA
                     MRGNRDAGTDQKQEPIP"
BASE COUNT          314 a          628 c          625 g          339 t
ORIGIN      
        1 cgtccggtaa attgccgacc ggcatgctgc aggcgctgga atgccacgtc gacctcgccg
       61 gtccgctgcg acgctcggtg atcggctgcc tggcgcggct cggcacaggc ggccctcagc
      121 agtccccgga caggctgcag gtgtcgctga cggagctgcc cctcacggga gaccatccgg
      181 gcccggtgct cggattgcac gcgcggaccg ccgccggcga cgtcattggc agcaagcgcc
      241 tgcaagcgcc gatcctggaa tgcagcctcg gtccgctgtt gctcgagttc gccctcgaat
      301 ggctctggat caggaatgat aagccgggct ttgctcgctg acggcgtcca ccagcgcggc
      361 cagatggtca ggattgacgt cgggcgttat cccgtggccc aggttgaaaa cgtgtcccgg
      421 cccatgaccg aagctggcca gggtcccggc gacttcacgg cggatcgtgt ccggatccgc
      481 cagcaacacg gcgggatcga gattgccctg cagcgcaacc cgcccgccga cgcgctgccg
      541 ggcttcggcc agcgtgatcg tccaatcgac gcccagcgca tcgcagcccg tttccgccaa
      601 cgcctccagt tgcgtgttgc agcccttgct gaagagaatg accggcaccg ggcgcccctc
      661 gctctcccgc tgcaggcctg cgacgatccg ggccatgtag gcgagtgaga attcccggta
      721 agccggcgca gacagggcgc cgccccaggt gtcgaaaatc tgcaccgcct gcgctccgct
      781 ggcgatctgg gcgttcaggt aatcggtaac ggtgtccgcc aatttaccga gaagctcgtg
      841 ggccaccgca ggctcctggt aaatcatctt cttgaccgtg ccgaaggttt tgctcgagcc
      901 gccttcgacc atgtaggtgg ccagcgtcca ggggctgccg ctgaagccga tcaggggcac
      961 cttgccgccc agttcccggc gggtgaggct tatggcatcc atcacgtagg cgagcgactc
     1021 gccgacatcc ggacgcggca gcttgcgtac tgcggaagct gaccgcacgg ggcgttcgaa
     1081 tcgcgggccc tcgcccgcgg caaacgacag cccgaggccc atggcgtcgg ggatggtgag
     1141 gatgtctgaa aagatgatgg ccgcgtccag ggcgaatcgg cgcaggggtt gcagcgttac
     1201 ctcgcaggcg agctggggtg actgacagag attcatgaac gagccggcct ctgcgcgggt
     1261 cttgagatac tccggcagat aacgcccggc ttgtcgcatg atccagacgg gagtgcgctc
     1321 caccggttcg tgccgcagcg cgcgcagaaa caagtcgttt ttgagtgccg tcatggtatg
     1381 ggttcctgtt tttgatctgt cccggcgtcg cggttgccgc gcatcgcctc tttgatttca
     1441 tcatgcccgc agcgatcgtc gatacgggcg cgtccgaccg catccagcaa gaccaggcgc
     1501 agtccgctgg caacggcctt cttgtccagt tccagcgccg cggccaaccg ctcgatggac
     1561 cggtcgggcg gaattctcac cggcaggccg cagcgctcca gcagggttgc caggcgctgg
     1621 gccgttccct tggagcacag gccccgggat tcgctcaggg aggcggcgac caccattccg
     1681 atggccacgg cttcaccatg gagaaagcgc tggtagcccg tctcggtctc gagcgcgtgc
     1741 ccgaagctgt gcccgaagtt gagcagcgcc cgcacccccg attccttttc gtcgcgtgcg
     1801 acgatttcgg ccttgttgag aaccgagcga tggatcagat actcgagggc ctcggcgtcg
     1861 cgggcattga cgggccctgc gttttgttcc aaccactcaa aaaaag
//
LOCUS       DBNHOX010000157         4106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_205487, whole genome shotgun sequence.
ACCESSION   DBNHOX010000157 DBNHOX010000000
VERSION     DBNHOX010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_205487"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /gene="hemB"
                     /locus_tag="AC16UZ_03360"
     CDS             <1..871
                     /gene="hemB"
                     /locus_tag="AC16UZ_03360"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003317357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR6245751.1"
                     /translation="GADQQIAVDSMPGINRLSIDNLLRHAEEAAKFGIPAIALFPYTQ
                     PDLRTPDGCEAWNPDGLIPRAVAALKEALPDLGVMTDAALDPYTTHGQDGIIDESGYV
                     LNDVTVEALCRQAAVCANAGADVIAPSDMMDGRVGAIRQMLESEGHDNTLIMAYSAKY
                     ASAYYGPFRDAVGSSATLGTGSKHTYQMDPANSDEALQEIALDLEEGADMVMVKPGMP
                     YLDIIRRVKDRFGVPTFAYQVSGEYAMHMAAVGNGWLDQSVVAESLLCLKRAGADGIL
                     TYFAFTVAAALAD"
     gene            985..1311
                     /gene="trxA"
                     /locus_tag="AC16UZ_03365"
     CDS             985..1311
                     /gene="trxA"
                     /locus_tag="AC16UZ_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HIR6245752.1"
                     /translation="MSDNIVHTSDSDFDNDVLSSDKPVLVDYWAEWCGPCKMIAPILD
                     EIAEEYSDRIKVCKLDIDANQATPPKYGIRGIPTLMLFKNGEVEATKVGALSKSQLAA
                     FLDSNI"
     gene            1555..2817
                     /gene="rho"
                     /locus_tag="AC16UZ_03370"
     CDS             1555..2817
                     /gene="rho"
                     /locus_tag="AC16UZ_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR6245753.1"
                     /translation="MNLTDLKRKPVGDLIDMAREMGLENLARSRKQEVIAAIVRKQAK
                     EGEDIYGEGTLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDSIAGKIR
                     PPKDGERYFALLKVDEINFSEPNSKKHKILFENLTPLFPDDRLVLERGNGSTEDLTAR
                     IIDLIAPIGKGQRALIVSPPKAGKTLVLQNIAQSIAANNPEVVLIVLLIDERPEEVTE
                     MQRMVQGEVVASTFDEPPSRHVQVAEMVIEKAKRLVEHQKDVVILLDSITRLARAYNT
                     IVPSSGKVLTGGVDAHALERPKRFFGAARNIEEGGSLSIIATALVDTGSKMDEVIYEE
                     FKGTGNQELHLERKIAEKRVYPAINIRRSATRREELLMGEEELQRVWILRKLLHDMDD
                     IAAIEFMIDKLKDTKTNEEFFALMRGGR"
     gene            2829..3527
                     /locus_tag="AC16UZ_03375"
     CDS             2829..3527
                     /locus_tag="AC16UZ_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase domain-containing protein"
                     /protein_id="HIR6245754.1"
                     /translation="MQARIVRRTGAPVQEIVLHVEPDFAFAAGQYLEILLPNGARIPL
                     SIASGPYRLPDVHLHYRSTPGIAEAVLFDELLQTSQTLDVAGPFGDVRLELPLAGPIL
                     IAAGGTGASQALGMLDALAQAAPRGEVPFEVSFLWCADREADLYRRDWLASLNADWLS
                     TTLIADSRRDRDNRALAWLRAQSARFRDRRIFLCGGPQFVYAAADALQAGGVPRGQLA
                     ADAFGYAPRPDNSG"
     gene            complement(3524..>4106)
                     /locus_tag="AC16UZ_03380"
     CDS             complement(3524..>4106)
                     /locus_tag="AC16UZ_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HIR6245755.1"
                     /translation="PGIRRAKLRDKAQLDELQKRAWSRRLAATTEDLTGLWAQMPKEL
                     RRNPELVAVLCDRLLEAGLANDAEAAVRGALEHEWRSELVQRYGVIVSDDVGRQLIVA
                     EGWLKSRPNDSALLLALGRICLMNEQWAKAREYLEASLRLRHTPEVYGELGRLCVAMG
                     DVDRGSQYLKQSVIGLPDLPLPEGEPGAAAGTG"
BASE COUNT          787 a         1379 c         1251 g          689 t
ORIGIN      
        1 gggcgccgat cagcagatag cggtggattc catgccgggc atcaaccggc tgtccatcga
       61 caaccttctc aggcacgcgg aggaagccgc aaagttcggg atccccgcca tcgctctgtt
      121 cccgtacacg cagccggatc tcagaacgcc ggatggttgc gaggcctgga acccggatgg
      181 cctgatcccg cgggcggtcg ccgccctgaa ggaagcttta cccgatctcg gcgtcatgac
      241 cgacgcggcc cttgatccct acacaaccca cggccaggac ggaatcatcg acgaaagcgg
      301 atacgtgctc aacgacgtca cggtggaggc gctctgccga caggcagccg tctgcgccaa
      361 cgccggcgcg gacgtcatcg cgccctccga catgatggac ggacgggtag gcgccattcg
      421 gcagatgctg gagagcgagg gtcacgacaa caccctcatc atggcctact cggcaaagta
      481 cgcgtcggcc tactacgggc cctttcggga tgcggtgggc tcgtcggcca ccctcggcac
      541 cggcagcaag catacctacc agatggaccc ggcaaacagc gacgaggccc tgcaggaaat
      601 cgccctggac ctggaggaag gggcggacat ggtgatggtc aaaccgggaa tgccgtacct
      661 ggacatcatc cgacgggtca aggaccggtt tggcgtgccg accttcgcct atcaggtcag
      721 cggcgagtat gcgatgcaca tggccgccgt cggcaatggc tggctcgacc aatccgtcgt
      781 cgccgagtcg ctgctctgcc tgaaacgagc gggcgccgac ggcattctga cctacttcgc
      841 gttcaccgtc gccgcagccc tcgcggatta gtggccgccc gccgatgtcg tgataaactg
      901 ccaccgttcc gccacggccc tgggccctct ccaagcgtcg cgtcccagac cgggtgactg
      961 tcatatcatt actggagcaa attgatgagc gacaacatcg ttcacaccag cgattccgac
     1021 ttcgacaacg acgtgctctc ttccgacaag cccgtcttag tggattactg ggcggagtgg
     1081 tgcggcccct gcaagatgat cgcgcccatt ctcgacgaga tcgccgagga gtactccgac
     1141 cgcatcaagg tctgcaagct agacatcgac gccaaccagg ccacgccgcc caagtacggc
     1201 atccgtggca tacctaccct catgctgttc aagaacggcg aggtcgaagc gaccaaggtg
     1261 ggcgcgttgt cgaagtctca gctggccgcc ttcctggaca gcaacatctg atatccacct
     1321 atcggccccg tcaggcggcg cgtaaacggg ttcacaaacc cggattgtgc gaccaaaggg
     1381 gtgcttgacg gggtctttca atacactaaa cttggctgca atcgctggag tcgaactcca
     1441 gcgcccaccc gccgccaccg cgcttcgccg gtcgcagcac ccaaagaaca gccataaaaa
     1501 tctaagaaca gtccgttcgt cgtttttaca acctgccggt ttgcgcttat gcccatgaac
     1561 cttacagacc tgaagagaaa acccgtcggc gacctgatcg acatggcccg cgagatgggg
     1621 ctggagaacc ttgcgcgttc gcgcaagcag gaggtcatcg ccgccatcgt ccgcaagcaa
     1681 gccaaagagg gcgaggacat ctacggggag gggaccctgg aaattctcca ggacggcttc
     1741 ggttttctgc gatccgcaga cagttcctat ctcgcggggc ccgacgacat ctacgtctcg
     1801 cctagccaga tacgccgatt caacctgcga acgggcgaca gcattgccgg caagatccgg
     1861 ccgcccaagg acggcgagcg ctacttcgct ctgctgaagg tcgacgagat caatttcagc
     1921 gagcctaact ccaagaaaca caagattctt ttcgaaaacc tcaccccgct cttccccgac
     1981 gatcggctgg tgctcgagcg cggcaacggc agcaccgagg atctgaccgc gcgcatcatc
     2041 gacctcattg cgcccatcgg caaaggccag cgagcgctca tcgtctctcc cccgaaagcg
     2101 ggaaagacgc tggttctgca gaacatcgct cagtccatcg cggccaacaa ccccgaggtc
     2161 gtgctgatcg tgctgctcat cgacgagcgg cccgaggaag tcacggaaat gcagcgcatg
     2221 gttcagggcg aggtggtggc gagcacgttc gacgagcccc cctcccgtca cgtccaggtg
     2281 gcggagatgg tcatcgagaa ggcaaagcgg ctggtggagc accagaagga cgtcgtgatt
     2341 ctgctggatt cgatcacccg tctcgcaagg gcctacaaca ccatagtgcc gtcgtccgga
     2401 aaggttctga ctggcggcgt cgacgctcac gcgctggagc gccccaagag attcttcggt
     2461 gcggcccgaa acatcgaaga gggcggcagc ctgtccatca tcgccaccgc cctggtggat
     2521 accggctcca agatggacga ggtcatctac gaggagttca agggaacggg caaccaggag
     2581 cttcacctgg aacgcaagat cgcagaaaag cgcgtctacc cggccatcaa catccgccgc
     2641 tcggccaccc gccgcgagga gctgctgatg ggcgaggaag agcttcagcg ggtctggatc
     2701 ctgcgcaagc tgctccacga catggacgac atcgccgcca tcgagttcat gatcgacaag
     2761 ctgaaggaca cgaagaccaa cgaggagttc ttcgcgctga tgcgcggcgg tcgctgaccg
     2821 agccaaccgt gcaggccagg atcgtccgcc gcacgggcgc acccgtgcag gagatcgtac
     2881 tgcacgtaga gccagacttc gctttcgctg cgggccagta cctggaaata cttcttccga
     2941 acggcgcgcg cattccactt tccatcgcct caggtcccta ccgcctcccc gacgtgcacc
     3001 tgcattaccg atccaccccc ggcatcgccg aggccgtcct tttcgatgag ctgctccaaa
     3061 cgagccaaac cctcgatgtc gccggccctt ttggcgacgt ccggctggag ttgcctctgg
     3121 ccggcccgat tctcatcgcg gcagggggaa cgggcgcgag ccaggcgctc ggcatgctcg
     3181 atgcgctcgc gcaggcagcg ccgcgcggtg aggtcccgtt cgaggtctcg tttctctggt
     3241 gcgcggaccg ggaggctgat ctgtatcgca gggactggct tgcctcgctg aacgcggact
     3301 ggctgtctac cacgctgatc gcggattcga gacgggatcg ggacaatcgg gcgctggcct
     3361 ggctgcgggc gcaatcggcg cggttccggg atcggcggat cttcctgtgc gggggcccgc
     3421 agttcgtcta cgcagcggcc gacgcgctcc aagccggcgg tgtgccccgc gggcaattgg
     3481 ccgccgatgc gttcggctac gcgccccgtc cggacaactc gggctagccg gtccccgccg
     3541 ccgcgcctgg ctcgccttcc ggcagcggca gatccggcag cccgatcacc gactgcttga
     3601 gatactggct gccgcggtcc acgtcgccca tggcgacgca caggcggccc agctcaccgt
     3661 agacctcagg cgtgtgccgc aggcgcaggc tcgcttcgag gtactcgcgg gccttggccc
     3721 actgctcgtt catcaggcag attcgaccca gggccagcag cagcgccgaa tcgttgggcc
     3781 gggacttcaa ccacccctcg gccacgatga gctgcctgcc cacatcatcc gagacgatca
     3841 caccgtaccg ctggaccagc tcgctgcgcc actcgtgttc cagggcgccc cgaaccgcgg
     3901 cctcggcatc gttcgcaagc cctgcttcaa gcagccggtc gcagagcacg gcaacgagct
     3961 ccgggttacg gcgcagctcc ttgggcatct gcgcccaaag acccgtgagg tcttccgtgg
     4021 tcgccgcgag ccgccggctc caggcgcgct tttgcagctc gtcgagctgg gccttgtcac
     4081 gcagctttgc gcgccggatg cccggc
//
LOCUS       DBNHOX010000158         3263 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1684396, whole genome shotgun sequence.
ACCESSION   DBNHOX010000158 DBNHOX010000000
VERSION     DBNHOX010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1684396"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..444
                     /locus_tag="AC16UZ_03385"
     CDS             31..444
                     /locus_tag="AC16UZ_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6245756.1"
                     /translation="MRHVGDILAEKSKDIVWIGPLESVYDALKLMNEKDVGAVLVQID
                     EYLVGILSERDYMRKVILDNHAAKQIKVSEVMTRDVVTVTREDTVVHCIALTKKHHVR
                     HLPVVEEGKAIGMISLRDLFLDVIHEKVGEVGDED"
     gene            complement(484..1422)
                     /locus_tag="AC16UZ_03390"
     CDS             complement(484..1422)
                     /locus_tag="AC16UZ_03390"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR6245757.1"
                     /translation="MAEIIARDAKFADPTTTADGKPRASVAPTGLTTLWVNTGTLCNL
                     TCENCYIESSPTNDRLAYISRAEVRAYLDEIAGSDLETEEIGFTGGEPFMNPDIMGML
                     EDSLGAGHRVLVLTNAMRPMMKHARGLLALGSRYGDALTLRVSVDHFEPDRHRQERGP
                     RSWRPMMEGLRWLSESGFAVHIAGRTRWGEDEHTLRAGFAALFAAEEIAVDAQDPASL
                     VLFPEMDTSADVPEITTECWDLLGRRPEDQMCASSRMVVKRRGAETPEVVACTLLPYD
                     ERFAMGPTLASSLRPAALNHPHCAKFCVLGGGSCSA"
     gene            1612..2376
                     /locus_tag="AC16UZ_03395"
     CDS             1612..2376
                     /locus_tag="AC16UZ_03395"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6245758.1"
                     /translation="MGRVEGKVAIVTGGASNPGLGRTTALTLAREGAIVVVTDVDLEG
                     AEATAAEIRDAGGRALALHQDVTDEGRWAEVIAATVDAHGRLDVLVNNAGIAVLMPLA
                     EMSLADWNRQIEVNLTSVFLGCKHAAPAMREAGGGSIVNLSSVAGLVGLPTCVAYGAA
                     KGGVRIMSKSVAVELAEHDIRCNSVHPGFIWTNMQAQSAGASAPGGGAMPRDRVPLGR
                     MGDPQDIANCVLYLASDESSYVTGAEFTVDAGMTAQ"
     gene            complement(2448..3248)
                     /locus_tag="AC16UZ_03400"
     CDS             complement(2448..3248)
                     /locus_tag="AC16UZ_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HIR6245759.1"
                     /translation="MPKSPDYPELEAARAGWRFQPEAECLRGKVILVTGAGDGIGAAA
                     AKTYACYGADVVLLGRTRSKLEAVFDWIEAETDTRPVIVPCDLRALDDASADALHDAI
                     ADGYGRLDGILHCASALGPKVPIALFPTADWQEVLQVNVTAPFLLTRTLLPLLNAAPA
                     ASVVLVSSTVGRKGRAYWGAYAVSKHALEGLMEVLADEHEHAGIIRVNSLNPGATRTA
                     MRAAAYPAENPASVPTPEARMDVFVSLMADASNGISGEALDAREWRGP"
BASE COUNT          532 a         1057 c         1124 g          550 t
ORIGIN      
        1 ttaggcggcg gcaatctggg gggatactga atgcggcacg taggggacat actggccgag
       61 aagtccaaag acatcgtctg gatcggcccg ctcgaatccg tgtacgacgc gctcaagctc
      121 atgaacgaaa aggacgtggg cgctgtgctc gtgcagatcg atgagtatct cgtcgggatc
      181 ctgtcagagc gggactacat gcggaaagtg attctggaca atcatgcggc caagcagatc
      241 aaagtctccg aggtgatgac ccgggacgtg gtcacggtta cccgggaaga caccgtcgtc
      301 cattgcatcg cgctgacgaa aaagcaccac gtccgtcatc ttcccgtggt ggaggaagga
      361 aaggccatcg gcatgatctc gctgcgcgac ctctttctag acgtcatcca cgagaaggtg
      421 ggtgaagtcg gcgacgaaga ctgacagcgg cgcgtccagc ggccgtccat cgtgtgagga
      481 gcgtcaggct gagcagctgc cgccgccgag gacgcagaac ttggcgcagt ggggatggtt
      541 cagcgcggcc ggccgcaggc tggaagccag cgtagggccc atggcgaagc gctcgtcgta
      601 gggcagcagc gtgcacgcga ccacctcggg ggtttccgcc cccctgcgct tgaccaccat
      661 tcgagagctt gcgcacatct gatcctccgg ccgccgtccc agcagatccc agcattcggt
      721 ggtgatctcg ggcacatcgg cgctggtgtc catctccggg aacagcacca ggctcgcagg
      781 atcctgcgcg tcgacggcga tctcctcggc ggcaaacagg gcggcgaagc cggcccgaag
      841 ggtgtgctcg tcctctcccc agcgggtgcg cccggcgatg tgaacggcga agccggactc
      901 gctcagccag cgtaacccct ccatcatcgg ccgccaggaa cgcgggcctc tctcctgtcg
      961 gtggcggtca ggctcgaagt ggtcgactga aacccgcagc gtcagagcgt ccccgtagcg
     1021 ggagcccagg gccagcagcc cgcgcgcgtg cttcatcatc ggccgcatgg cgttggtgag
     1081 caccagaacg cgatggcccg cccccaggct gtcctcgagc atgcccatga tgtcggggtt
     1141 catgaatggc tcgccgccgg taaagccgat ctcctcggtt tcgaggtcgg agccggcgat
     1201 ctcgtcgaga tatgctcgaa cctcggcgcg agagatgtag gccaggcggt cgttggtcgg
     1261 gctggactcg atgtagcagt tctcgcaggt caggttgcac agggtgccgg tattgaccca
     1321 cagggtcgtc agtccggttg gagccacgga tgccctgggc ttgccgtctg ccgtggtggt
     1381 cgggtcggcg aacttcgcgt cccgcgcgat gatctcggcc atctctgatt gcccttgcgc
     1441 cggcgtttgc ggcgctcgtc ctgctcgatc ggtctctcat gctgacaggc cgccttctcc
     1501 cccgcaacgg gcgtacacgg gttcgtccgg aaagacgagc tttgtccgac acaagccggt
     1561 ttgccatact cgcgacctct tttctgcagt cgacacaccg ggagcggcga tatgggacga
     1621 gttgagggca aagtggccat cgtgacgggc ggggcgtcca atcccggcct cggccgcacg
     1681 acggcgctga cgctggcgcg cgagggcgcg atcgtcgtgg taaccgacgt ggatctggaa
     1741 ggtgccgagg ccaccgcggc ggagataagg gatgcgggag gaagggcgct ggcgctgcac
     1801 caggacgtca ccgacgaggg ccgctgggcc gaggtcatcg ccgctacggt ggatgctcac
     1861 ggcaggctgg acgtcctggt caacaacgcc gggatcgcgg tgctcatgcc ccttgccgag
     1921 atgtctctgg cggactggaa ccggcagatc gaggtcaacc tgaccagcgt cttccttggc
     1981 tgcaagcacg cggcaccggc aatgcgggag gcaggcggcg gctccatcgt caacctctcg
     2041 tccgtggccg ggcttgtggg cctgcccacc tgcgtcgcct acggcgcggc caagggcggg
     2101 gtccggatca tgtccaagag cgtcgccgtg gagctcgccg agcacgacat tcgctgcaac
     2161 tcggtccacc ccggattcat ctggaccaac atgcaggctc agtccgcggg tgcgtcagcc
     2221 cccggcggtg gcgcgatgcc ccgggaccgg gtgccgctgg gacgcatggg tgatccccag
     2281 gacatcgcca actgcgttct ctacctcgcc tccgacgagt cgagctacgt caccggcgcc
     2341 gagttcaccg tggatgcggg tatgacggcg cagtagctgg ccggctccgt tcaggtcctc
     2401 cggtaccgcg ttggtttgag atcggcccgc cgcagcgagc agggctgtca cggaccgcgc
     2461 cactcacgcg catccagcgc ctccccgctg atgccgttcg aggcgtccgc catcagggag
     2521 acgaacacgt ccatccgcgc ctctggcgtc ggcacgctgg cggggttctc ggcaggatag
     2581 gcggcggccc gcatggccgt gcgcgtggcg cccgggttca ggctgttcac gcggatgatg
     2641 ccggcgtgct cgtgctcgtc ggccagaacc tccatcaggc cctccagcgc gtgcttggaa
     2701 acggcgtagg ctccccagta ggcccggccc ttgcgcccca ccgtggagga gaccagcacg
     2761 accgacgcgg ctggtgccgc gttcagcagg ggcagcaggg ttcgggtcag caggaagggt
     2821 gcggtgacgt tgacctgcag cacctcctgc cagtccgccg ttggaaacag ggcgatgggc
     2881 actttcgggc cgagggccga ggcgcagtgc aggatgccgt cgagccggcc gtagccgtcg
     2941 gcgatggcgt cgtgcagggc gtcggcgctt gcgtcatcca aagctctgag atcgcagggg
     3001 acgatcaccg gccgggtgtc cgtttccgcc tcgatccagt cgaacacggc ttccagtttg
     3061 gatcgcgtgc ggcccagcag caccacgtcc gcaccgtagc aggcgtacgt cttcgccgcc
     3121 gcggcgccga tcccgtcgcc ggccccggtg accaggatca ccttgccgcg caggcactcg
     3181 gcttcgggct ggaaccgcca gccggcgcgt gccgcctcca gctcgggata gtcaggactt
     3241 ttcggcatag agcatgtagt tca
//
LOCUS       DBNHOX010000159         2501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1555509, whole genome shotgun sequence.
ACCESSION   DBNHOX010000159 DBNHOX010000000
VERSION     DBNHOX010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1555509"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1008
                     /gene="ltaE"
                     /locus_tag="AC16UZ_03405"
     CDS             19..1008
                     /gene="ltaE"
                     /locus_tag="AC16UZ_03405"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HIR6245760.1"
                     /translation="MRTVDLRSDTVTRPTPAMRDAIYAAEVGDDVYGDDPTVNALEAL
                     AAEMLGKEAALFVTSGTQANLVALLTHCGRGDEYIAGATAHTYWYEAGGAAALGGIQP
                     QTLPFEPDGTLDLTAVAKLVKPDDVHFARTRLLCLENTQAGRAAGPAYFAAARTFCDE
                     RGLGLHLDGARLFNAAIATGCEAQDIARHCDSVSICLSKGLGSPAGSLLLGSADFIRE
                     ARRWRKMLGGGLRQAGILAAGGIYALNHHVDRLAEDHSRAARLAQAVNERYPDGAEAH
                     TNMVFLNLAEAEVDRLKDHLADRGIVIRGPRWVTHLDVSDEDVARVVDAIRAS"
     gene            complement(1029..2267)
                     /locus_tag="AC16UZ_03410"
     CDS             complement(1029..2267)
                     /locus_tag="AC16UZ_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HIR6245761.1"
                     /translation="MQAILGIGFLLGLAWILSESRRTVRWRPVMVGLVVQLGLAALLL
                     RVPVVAESLLVLNKLVYAVEAATGAGAGFVFGYLGGGDAPFTVSAQGAMYVFAFRVLP
                     QILVFSVLVALLWYWRVLPAVIRGFGYLLRRALDVGGAVGTAAASSVFLGMVEAPLVI
                     RAYLERLSRSELFTVMTCGMSTVAGSIMVLYASVLGDVITGALGHILAASVINVVGAV
                     VVSRIMIPADDATAEEDGASGLSYVSNMDAITRGTADGLRLAVNVGAMLVVLVSLVAL
                     VNQGLGAVTVWEGPLSLERMMGWVFSPGAWLIGIPWGEAQAAGALLGTKLILNELVAY
                     LQLAGLPEGSLSDSSRLVMTYALCGFANFGSLGILLGGLNTLVPERRKEILSIGPKTL
                     ISGTIVTCNTGAIVSLIGLF"
     gene            complement(2294..>2501)
                     /locus_tag="AC16UZ_03415"
     CDS             complement(2294..>2501)
                     /locus_tag="AC16UZ_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245762.1"
                     /translation="AKESVYKAVNPIVGKYIGFHEVEVDVTFAEQRFSARYVGEHAPN
                     ALLENGAGYFLFAEQHVFSVFIIP"
BASE COUNT          489 a          874 c          772 g          366 t
ORIGIN      
        1 atctaggagg cccgacccat caggaccgtc gatctccgca gcgacaccgt tacccggccg
       61 accccggcca tgcgtgatgc catctacgca gcggaagtag gcgacgacgt ctacggcgac
      121 gatcccaccg tcaacgccct ggaggccctg gccgcggaga tgctgggcaa ggaggccgcc
      181 ctcttcgtca ccagcgggac ccaggccaac ctggtggcgc tgctcaccca ctgcgggcgc
      241 ggcgacgagt acattgccgg cgccacggcc cacacctact ggtacgaagc cggcggggcc
      301 gccgctctcg gcggcatcca gccccagacc ctgcccttcg agccggacgg caccctggat
      361 ctcacggcgg tagccaagct ggtgaagccc gacgacgtcc acttcgcccg gacgcgtctg
      421 ctctgcctgg agaacactca ggccgggcgc gcagccggtc ccgcctactt cgccgcggcc
      481 cggacgttct gcgatgaacg tgggctgggt ctgcacctcg acggcgcgcg gctgttcaac
      541 gcggccatcg ccaccgggtg cgaggctcag gacatcgcca ggcactgcga ttcggtctcc
      601 atctgcctgt ccaaaggcct cggctcgccg gcaggctccc tgctgctggg tagcgccgac
      661 ttcattcgcg aagcaaggcg ctggcgcaag atgctgggag gcgggcttcg ccaggccggc
      721 atcctcgccg ctgggggcat ttacgcgctc aatcaccacg tggatcgcct ggctgaggac
      781 cactcacggg ccgcgcgtct ggcgcaggcc gtcaacgagc gataccccga cggtgctgaa
      841 gcacacacca acatggtgtt cctgaacctc gccgaggcgg aagtggatag gcttaaagac
      901 catctggccg atcgtggcat cgtcatccgc gggccgcgct gggtgaccca tctcgacgtc
      961 tccgacgagg acgtcgcgcg cgtcgtcgac gccattcggg caagctgagg cgccttggtc
     1021 ttaccgcttc agaacaggcc gatgagcgac acgattgccc cggtgttgca ggtaacaatc
     1081 gtaccggaaa tcaacgtctt aggtccgata cttaatatct ctttgcgtcg ttccggaacg
     1141 agcgtattca ggcccccgag cagaatgccc aggctgccga aattggcaaa gccgcacagc
     1201 gcgtaggtca tcaccagccg gctcgagtcg ctcaagcttc cctccggcag cccggcgagc
     1261 tgcagatagg ccaccagctc gttcaggatc agcttggtgc cgagcagcgc cccggccgcc
     1321 tgggcttctc cccagggaat cccgatcagc caggctccgg gggagaacac ccatcccatc
     1381 atccgctcca gagaaagcgg gccctcccac accgtcaccg cgcccaggcc ctggttcacc
     1441 agagcgacaa ggctcacgag aaccacgagc atggcgccga cgttcacggc cagacgcaga
     1501 ccgtccgccg tgccgcgcgt gatggcgtcc atgttggaga cgtagctgag accggaagcg
     1561 ccgtcctctt ctgccgtggc gtcgtccgcc ggaatcatga tgcgcgaaac gacgacggcg
     1621 ccgaccacgt tgatcacgga agcggccagg atgtggccga gcgcgcccgt aatgacgtcg
     1681 ccaagcacgc tggcgtagag gaccatgatg gatccggcca cggtggacat gccgcaggtc
     1741 atgaccgtga agagctccga acgggacaag cgctcgagat aggcccggat caccagcggt
     1801 gcttccacca tgccgagaaa cacgctggag gcagccgccg ttcccacggc gccgcccaca
     1861 tcgagtgcac gacggagcag gtagccgaac ccgcggatca ccgccggcag cacgcgccag
     1921 taccacagca gcgccacgag cacgctgaaa acgaggatct gcggcagaac gcgaaacgca
     1981 aagacgtaca tggccccttg agcgcttacc gtgaacggcg catccccacc gcccagataa
     2041 ccgaacacga accccgcccc ggccccggtc gccgcctcca ccgcatagac gagcttgttg
     2101 agcaccagca gagattcggc caccacggga acccgcagca gcagcgcggc aaggccgagc
     2161 tgaacgacga gacccaccat cacgggccgc cagcgcacgg tgcgccggct ctcgctcaga
     2221 atccacgcta gccccagcag aaagccgatt ccgagtatgg cctgcattca gactctgcct
     2281 tcgaggtagg ggttcacgga atgatgaaca cgctgaagac gtgctgctcg gcgaaaagga
     2341 agtagcccgc gccgttttcg agcaaggcgt tgggagcgtg ctcgcccacg tagcgggcag
     2401 aaaagcgctg ctctgcgaac gtcacatcca cctccacctc gtgaaacccg atgtacttgc
     2461 cgacgatcgg gttgaccgcc ttgtagacgg attctttggc g
//
LOCUS       DBNHOX010000160         2733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_12644, whole genome shotgun sequence.
ACCESSION   DBNHOX010000160 DBNHOX010000000
VERSION     DBNHOX010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_12644"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1194)
                     /locus_tag="AC16UZ_03420"
     CDS             complement(43..1194)
                     /locus_tag="AC16UZ_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6245763.1"
                     /translation="MSTEQDTDVIRTIVSQDLLDVLVRVGLIGILLVMSFRIFSPFLS
                     ILLWALVLAVSLYPVHLKLVGKMGGRQGASATVIVIVGMLLIGTPTIALGSLFAGYIH
                     ETYQAFATNSVTVNPPRPEVAEWPIIGERMYAAWSAAASDMPAFLEELQPQLGVLTDR
                     ALSTAASTMGDVLLFLGALIIAGIMMAFGQSGSDAMGRILSRLSGPENGPRLHNLTTA
                     TIRSVATGVIGVAFIQALLLGVGFAFAGIPAAGLLALVVMLVGIVQLPALLVTLPVIA
                     YLWWGGDASTTSNVIWTIYLLVAGMADNVLKPLLLGRGVDAPMPVILIGALGGMVSAG
                     IVGLFVGAVILAVGYQLFMDWVNGGAGAETSPEGSAAEQEGGTVPEPRA"
     gene            complement(1261..>2733)
                     /gene="selD"
                     /locus_tag="AC16UZ_03425"
     CDS             complement(1261..>2733)
                     /gene="selD"
                     /locus_tag="AC16UZ_03425"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR6245764.1"
                     /translation="GVMAASWPAAAGLATDVSGFIEVDRCLRSTSHESVYAAGDVACL
                     IDQPRPKSGVYAVREGPALTRNLRRAIVGRPGIPFRAQKRFLSLIGTADGSAVAARGP
                     WAASGRWAWRWKDWIDRRFVARFNRLPVMTEPGWSLPESLRDEAPDAMRCGGCGAKLG
                     ASPLMRVLERLPPQVSGNVRVGIGDDAAILGATGGETILSVDGFRSLVSDPYLFGRIT
                     AHHALNDIIAMGGKGTAALAMASVPLMSEALMEEDLFQLLRGAVDVLNAHEVPLVGGH
                     SSEGAELSLGLTVAGVPDGPTLTKGGAEPGDALVLTKPLGTGSILAGQMRGAVRSDSV
                     SAAIASMDASNASALVVFRRYGIHAATDVSGFGLLGHLGEMLRASALGVRLDLNAVPF
                     IEGAVEAVSAGAVSSLQDNNELALADFALEGCEPGDATVRLLVDPQTSGGLLGCVPEA
                     EAEACVRDLREVGYPRAAVIGRVLSNGWTVTAEREARPLS"
BASE COUNT          515 a          969 c          860 g          389 t
ORIGIN      
        1 agcaccgacg ccgtcggcga cttgccgggc gagaacacgt tgtcaggctc ggggttccgg
       61 gacggtcccg ccctcttgct ctgctgcaga gccttccgga gacgtttcgg ctccggcgcc
      121 gccattgacc caatccatga agagctggta gcccaccgcc aggatcacgg cgccgacgaa
      181 cagccccacg atgccggcgg acaccatgcc gcccagcgcg ccgatcagta tcaccggcat
      241 gggcgcgtcg acgccgcgac ccagcagcag gggtttgagg acattgtcag ccataccagc
      301 tacgagcagg tagatcgtcc atatgacgtt gctggtcgtg gacgcatcgc cgccccacca
      361 cagataggcg atcaccggaa gcgtgaccag cagcgcgggc agctggacga tgccgaccag
      421 catcaccacc agcgccagca aacctgcggc tgggatgccc gcaaaggcga atcccacgcc
      481 gaggagcagg gcctgaatga acgccacgcc gatcacgccc gtggccaccg agcgaatcgt
      541 cgccgtcgtc aggttgtgca ggcggggccc gttttccggg ccggacagcc gactgaggat
      601 ccgacccatg gcgtcgctgc ccgactggcc gaacgccatc atgatgcccg ctatgatgag
      661 ggcaccgaga aagagcagca catcgcccat ggtgctggcc gccgtgctca gcgcacgatc
      721 cgtcagaacg ccgagctggg gctgcaattc ctccaggaag gcaggcatgt cgcttgccgc
      781 cgccgaccag gccgcataca tccgctcccc gatgatgggc cactcggcga cctccggcct
      841 cggtgggttg acggtgaccg agttggtcgc aaacgcctga taggtttcgt ggatatagcc
      901 ggcaaaaagg ctgcccaggg ctatggtcgg cgtgccgatc agcagcatcc cgacaatgac
      961 gatcacggtt gccgacgcgc cctgccgacc acccatcttg ccgacaagtt tcagatgcac
     1021 cgggtacagc gacacagcga gcacgagcgc ccacaggaga atgctgagaa acggagaaaa
     1081 tatccggaag ctcatcacca gaaggatgcc gatcagcccc acccggacca gcacgtccag
     1141 caagtcctgg gaaacgatgg tgcggatgac gtcggtgtcc tgctcggtgc tcatttccac
     1201 tcccccaact gatgccaaac ggcgaatcat acgcgaggac gcgcatggtc gtctcgcggc
     1261 tcagctgagc gggcgcgcct ctcgttcggc ggtaacggtc cagccgttcg acagcacccg
     1321 gcctatgacg gccgcacgcg ggtaaccgac ctcgcgcaga tcccgaacgc aggcttcggc
     1381 ctccgcctcc ggcacgcacc cgagcagccc gccggaagtc tgagggtcga cgagcagacg
     1441 cacagtcgca tcgcccggct cgcagccttc cagcgcgaaa tccgcgaggg cgagctcgtt
     1501 gttgtcttgc agcgagctga cggcaccggc gctgacggcc tcgaccgcac cctcgatgaa
     1561 aggcaccgcg ttgaggtcga gccgcacacc cagggcggac gccctgagca tctcccctag
     1621 gtgaccgagc aacccgaagc ccgacacgtc ggtggcggca tggatgccgt agcgacggaa
     1681 cacgaccaga gccgatgcgt tcgatgcgtc catgctggca atagcggcgg agacgctgtc
     1741 cgagcgaacg gcgccgcgca tctgcccggc caggatcgaa cccgtgccga gcggcttggt
     1801 gagcaccagc gcgtcccccg gttccgcccc gcccttggtc aaggtcggtc cgtcgggaac
     1861 gcccgcaacg gtaagaccca ggctcagctc ggctccctca ctcgagtgcc cgcccaccag
     1921 gggcacctcg tgcgcattga gcacgtccac cgcgccgcga agcagctgaa agagatcttc
     1981 ctccatgagc gcttcggaca tcagaggcac gctcgccatc gctagggcag ccgtaccctt
     2041 accgcccatg gcgatgatgt cgttcaaggc gtggtgggcc gttatgcggc caaacaggta
     2101 agggtcggag acgaggctgc ggaagccgtc gacggagagt atcgtctcgc caccggtcgc
     2161 gccgaggatg gccgcgtcat cgccgatgcc gacccgcacg ttcccggaca cctgaggcgg
     2221 caggcgctcg agcacgcgca tcagcgggct cgcgccgagc ttcgccccgc agcctccgca
     2281 tcgcatggcg tccggcgctt cgtcacggag actctctggc agcgaccagc ccggttccgt
     2341 catgaccggc agccggttga agcgcgccac gaaccggcgg tcgatccaat ccttccatcg
     2401 ccaggcccag cggccgctgg ccgcccaagg gccgcgagcg gcgacagcgg acccgtcggc
     2461 agttccgatg aggctcaaga agcgcttctg tgcccggaac ggaatcccgg gccgtccgac
     2521 gattgcccgc cttagattgc gggtcagcgc cgggccctcc cgaacggcgt acacgcccga
     2581 cttgggcctc ggctgatcga ttagacaggc cacgtccccg gccgcgtaaa cgctctcatg
     2641 actcgtggac cgcaggcagc gatcaacctc gatgaacccg ctgacatccg tggccagccc
     2701 agccgcagcc ggccacgacg ctgccatcac ccc
//
LOCUS       DBNHOX010000161         5442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1041871, whole genome shotgun sequence.
ACCESSION   DBNHOX010000161 DBNHOX010000000
VERSION     DBNHOX010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1041871"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..683)
                     /locus_tag="AC16UZ_03430"
     CDS             complement(72..683)
                     /locus_tag="AC16UZ_03430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR6245765.1"
                     /translation="MLRRGPLTTGSAPVGGFWYTRDGLDAPDVQVHFASGATLYNEEG
                     RIRAMPVPAMTAVVNQSRPESVGSIHITSADPRQPPAIRPNYLDSELDRDTLVRGLGM
                     IIDIFESTAMRPYVTERLLPEPGVTSDEALLEYIRGDAATTYHPTSTCRIGSVVDENL
                     KVLGVSGLRVVDASVMPFVVSGNTNAATIMIAEKAADMILAAS"
     gene            complement(789..1658)
                     /locus_tag="AC16UZ_03435"
                     /pseudo
     CDS             complement(789..1658)
                     /locus_tag="AC16UZ_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012935.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
     gene            complement(1697..2200)
                     /locus_tag="AC16UZ_03440"
     CDS             complement(1697..2200)
                     /locus_tag="AC16UZ_03440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6245766.1"
                     /translation="MTLQATFNTSKGAIQVSLMPERAPVTVANFANLARRGFYDGLKF
                     HRVIADFMVQGGCPLGTGTGGPGYRFEDEFDSEVRHDRPGRLSMANAGPGTNGSQFFI
                     THVPTPWLDGNHTIFGEVQGPEDQAVVDSIAQGDVIESVVIEGDLDALLEAHADRIEE
                     WNRALDR"
     gene            2389..3426
                     /locus_tag="AC16UZ_03445"
     CDS             2389..3426
                     /locus_tag="AC16UZ_03445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6245767.1"
                     /translation="MRLLLVTDAWFPQVNGVVRTLSTTMENLARLGHETEVIAPARFK
                     SVPCPTYPEIRLALCTPGRVGNMIAEFAPDAVHVATEGPLGLAARWWLKRRGIPFTTS
                     FHTMFPQYVRLRFGLPEAWTFAAMRRFHGAATQTMYSTPRLRDMLRGQGFANLVGWVR
                     GVDTELFKPGPAEPLDLPRPLQMYVGRVAVEKSLEDFLSLDTPGTKVIIGDGPQRGEL
                     AARYPQARFLGPRYGDDLVRCYRAADVFVFPSRTDTLGLVMLEAMACGVPVAAFPVQG
                     PLDVVADSGAGVLHDDLGTAIAQAIGIDPEICRARAMEHSWDQSVREFHANLVPIGQT
                     AYHFRGEGETA"
     gene            3423..4643
                     /locus_tag="AC16UZ_03450"
     CDS             3423..4643
                     /locus_tag="AC16UZ_03450"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6245768.1"
                     /translation="MKILILGGDGFCGWPTALHLSAQGHCVVIVDNLSRRNIDNELEV
                     QSLTPIRPLGERIGTWYELTGREIQFVRMDVATEYDRLLELIRSHRPDAIVHFAEQRA
                     APYSMKSSWHKRYTVNNNLNATNNVLAAIVDSRQDIHLVHLGTMGVYGYSSVGVKLPE
                     GYLNVKMTNDDGEEVEQEILYPANPGSIYHMTKTQDQLFFAFYNKNDQVRVTDLHQGI
                     VWGTQTSETRLDERLINRFDYDGDYGTVLNRFLMQAAIDYPLTVHGSGQQTRAFIHLQ
                     DTVRCVELAISNPPAAGERVKILNQLTETHRLIDLARLVSEMTGVPIEHVPNPRREAD
                     ANDLAASNEGLVGLGLQPTTLKGGLLEEVTDIARRYAERCDRTRIPCMSHWTTDIAGD
                     IEVIVPSGEASTAG"
     gene            complement(4640..5059)
                     /locus_tag="AC16UZ_03455"
     CDS             complement(4640..5059)
                     /locus_tag="AC16UZ_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR6245769.1"
                     /translation="MTYDPDNVFAKIIRGQAPAHRVYEDAWTLAFMDLMPQTDGHTLV
                     IPKSEAVSLLDVDSEALARTIQTTQKIAAAVKAAFGAHGVMVAQLSGAAAGQTVFHLH
                     FHVLPRLHGADFEIHAREVAPAEVLAEHAERIRRALG"
     gene            5167..>5442
                     /locus_tag="AC16UZ_03460"
     CDS             5167..>5442
                     /locus_tag="AC16UZ_03460"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6245770.1"
                     /translation="MQLKGQVAVVTGGASGMGRDTVLRFLAEGARVVIADFNARTGQA
                     VLEEARAAGFGESTRFVRTDVAREADVEAMCGLAVEAFGRLDVVFNNA"
BASE COUNT          952 a         1816 c         1757 g          917 t
ORIGIN      
        1 cgcagcccgc cgaacccgcg ccgaccacga tgtaatcgta tgacatagcg ctttcgcctt
       61 tgttgggggc ttcaggatgc cgcgagaatc atgtccgcgg ccttctccgc gatcatgatg
      121 gtcgccgcat tggtattgcc cgaaaccacg aaaggcatga cggacgcatc cacgacccga
      181 agcccggaga cccccagaac cttcaggttc tcatccacca cggatccgat gcggcacgtg
      241 ctcgtgggat ggtaggtggt ggcagcgtcg ccgcggatgt actcgagcag cgcctcgtca
      301 gaggtgacgc cgggttcggg caggagacgc tcggttacgt acggccgcat cgccgtgctc
      361 tcgaagatgt cgataatcat tcccagaccc ctgacgaggg tatcccggtc gagctcgctg
      421 tcgagatagt tgggccggat ggccggcggt tgcctcgggt cggcgctggt gatgtggatc
      481 gagcccacgg actcaggccg actctggttg accaccgcgg tcatggcggg caccggcatg
      541 gcccggatac gcccttcctc gttgtaaagc gttgcgccgg aagcgaaatg aacctgcacg
      601 tcgggggcgt cgaggccgtc acgcgtgtac cagaagccgc cgacaggagc cgatccggtg
      661 gtcaacggtc cacgcctgag catgagccat ttcaaggcgg caacgccgac ccgccacccc
      721 tgcacctccc ggttgatcga ttgctcggga tgagtcccgt aaaccgtctt ggtcagaagg
      781 tgatcctgga ggttctcgcc gacctcgggc aggtcaccga cgacctcgat gccgagcccc
      841 cgcagccgct cgccgtcccc gactccggac agctcgagga gctgcggcga gttgatcgcg
      901 ccgctggcca ggatgacttc cctggcagct acccgcgtgg cgggggatcg ggcccgctcg
      961 atctccaggc ccgtggccgc cttcccgtcg aagcataccc gcctgacgag gacgcccgtc
     1021 atcacgtcga ggttgggccg gcgtctggcg ggaccgaggt aggcggttgc cgtgctccag
     1081 cgccgacccc ggagctgatt gaactggtag taaccgcaac cggcctgctc ctgcccgttg
     1141 aaatcgtcgt tgcgcggaat gcccagctcc gtcatgccgt cgatgaacgc atccatggcc
     1201 gctcgcctgt cgcgcacgtc ctggacgtgc aggggtccct ccgcgccgtg aaactcgtcg
     1261 gccacccgct gctggcgctc ggccttacgg aaatacggaa gcacctcctc gtagctccag
     1321 ccccgagccc ccgactgggc ccagcggtcg aagtccaggg gaacgccgcg aatgtagatc
     1381 atcccgttga tggcgctgga gccacccaga accttgcccc gaggccagta gatggagcgc
     1441 ccgttcaggg ccggctcggg ctcagtggaa aaaccccagt ccaccttcgg gttgcggaag
     1501 ttggtcgccc accccgcagg aatgtgcagc agcgggttcc cgttgcccgg ccccgcctcg
     1561 atcaggagca cgcgcgtctc cgggtccgcc gacaaccggt tggccagaac gcagccggcc
     1621 gagccggctc ccacgatggc gtagtcgtag gcgtccatga ccggtttcgc gcttcagggg
     1681 ctcgtgacgg ggcggatcaa cggtccaggg cccggttcca ttcctctatg cgatccgcgt
     1741 gggcttctag cagagcgtcc aggtccccct cgatcaccac gctttcaatg acgtcgccct
     1801 gggcgatgct gtccaccacg gcctgatctt ccggcccctg aacctcgccg aagatggtgt
     1861 ggttgccgtc gagccaggga gtgggtacgt gggtgatgaa gaactgcgag ccgttggttc
     1921 cggggccggc gttggccatg gatagccgac cggggcgatc gtggcggacc tccgagtcga
     1981 actcgtcctc gaagcggtag ccgggaccgc cggtccccgt acccagcgga cagcctccct
     2041 gaaccatgaa gtcggcgatc acgcgatgga atttcagacc atcgtaaaaa ccccgccggg
     2101 caaggttcgc gaagttcgct acggttaccg gagcccgctc gggcatcagg gaaacctgaa
     2161 tggctccttt ggaagtgttg aaggtggctt gcagagtcac gttagctcct gtggggtaac
     2221 ttgaagaggc tcgagagccg tatcggcggc cagtgaaaca caacgggcag gcgttttcga
     2281 gcggcgccaa cggcccaggc ggccaaaccc ccagcgggtt gtagccgccc tcccagtccc
     2341 gctactatcc tcgccaagcg accgcaccgc ggcgattaga gagataacgt gcggctactg
     2401 ttagtcacag atgcctggtt cccacaggtg aacggcgtgg ttcgaacgct gtccaccacc
     2461 atggaaaatc tcgcgcggct gggccacgag accgaggtga tcgcccctgc gcgcttcaaa
     2521 agcgtcccct gtccgaccta cccggagatc cggctcgcgc tgtgcactcc gggccgggtc
     2581 gggaacatga tcgccgagtt cgcgccggac gcggtgcacg tcgccacgga agggccactg
     2641 gggctggccg cccgctggtg gctcaagcgc cgcggcatac cgttcaccac ctcgttccac
     2701 accatgttcc cgcagtacgt caggctccgg ttcgggctgc ctgaagcatg gacgtttgcg
     2761 gccatgcgcc gcttccacgg ggccgcgact cagaccatgt actccacgcc ccggctgcgc
     2821 gacatgctcc gcggccaggg cttcgccaac ctcgtcggct gggtgcgcgg cgtggacacg
     2881 gagctgttca agccgggtcc ggcggaaccc ctcgatctgc cgcggccttt gcagatgtac
     2941 gtcggccggg ttgccgtgga gaagagcctc gaggactttc tgtccctgga cacccccggc
     3001 accaaggtga tcatcggcga cgggccccag cggggagagc ttgccgcacg atatccccaa
     3061 gcacgtttcc tgggcccccg ctacggcgac gacctggtcc gctgctaccg ggcagccgac
     3121 gtgttcgtgt tcccatcgcg caccgacacc ctgggactgg tgatgctgga agcgatggcc
     3181 tgcggcgtgc cggtcgctgc gtttccggtg cagggcccat tggacgtggt cgccgacagc
     3241 ggcgcaggag tgctgcacga cgacctcggc accgccatcg ctcaggccat cggcatcgac
     3301 cccgaaatct gccgagcccg ggccatggag cactcgtggg atcagtccgt gcgcgaattc
     3361 cacgcaaacc tggtacccat cggacagacc gcctaccatt tccgcggcga aggggagacc
     3421 gcatgaaaat tctcattctc ggcggggacg gcttctgcgg ctggcctacc gccctgcatc
     3481 tgtcggcaca gggtcattgc gtggtcatcg tcgacaacct ctccaggcgt aacatcgaca
     3541 acgagctcga agttcagagc cttaccccaa tccgcccgct gggcgagcgc atcggcacct
     3601 ggtacgagct gacgggacgg gaaatccagt tcgtgcgtat ggatgtggcg acggagtacg
     3661 accggttgct ggagctgatc cgcagccatc gcccggacgc catcgtgcac tttgccgagc
     3721 agcgtgccgc accgtactcc atgaagtctt cctggcacaa acgctacacg gtcaacaaca
     3781 acctcaacgc caccaacaac gttctggccg ccatcgtcga cagccgccag gacatccacc
     3841 tggtgcacct gggcaccatg ggcgtctacg gctactccag cgtgggcgtg aagctgccgg
     3901 aaggctatct gaacgtgaag atgaccaacg acgacggcga ggaggtggag caggagatcc
     3961 tctatccggc gaacccgggc agcatctacc acatgaccaa aacccaggat cagctgttct
     4021 ttgccttcta caacaagaac gaccaggtgc gcgtcaccga cctgcaccag ggcattgtct
     4081 ggggtaccca gacgagcgag acccgcctgg acgagcggct gatcaaccgc tttgactacg
     4141 acggcgatta cggcacggtc ctcaaccgtt tcctgatgca ggccgccatc gactaccccc
     4201 tcaccgttca cggcagcggg cagcagacgc gagccttcat ccaccttcag gacacggtgc
     4261 gctgcgtcga gctcgccata tccaacccgc cggccgcggg cgagcgggtg aagattctga
     4321 accagctgac ggaaacccac cggctgatcg atctcgcccg gctggtctcg gagatgacgg
     4381 gcgtgcccat cgagcacgtg ccgaatccgc gtcgggaggc cgacgcaaac gatctggccg
     4441 cgagcaacga agggctggtg gggctcggcc tgcagccgac gacgctgaaa ggcggcctgc
     4501 tcgaagaggt gaccgacatc gcccggcgct atgccgaacg ctgcgaccgg acgcgcatcc
     4561 cctgcatgtc ccactggacg acggacattg cgggggacat cgaggtgatc gtccccagcg
     4621 gcgaggcttc gaccgccggc tagccgagcg cgcggcgaat acgctctgcg tgctcggcga
     4681 gcacctcggc aggcgccacc tccctggcgt gaatttcgaa gtcggcgcca tgcagcctgg
     4741 gcagcacgtg aaagtgaagg tgaaagaccg tctgcccggc ggccgccccg ctcagctgag
     4801 ccaccatcac cccatgggcg ccaaatgccg ccttcaccgc cgcggcgatc ttctgggtcg
     4861 tctggatcgt ccgggcaagc gcctcggagt ccacatccag caggctcacc gcctcgctct
     4921 tgggaatgac gagggtgtgg ccgtccgtct gcggcatgag gtccatgaac gccagcgtcc
     4981 aggcgtcctc gtaaacccgg tgggcgggcg cctggccgcg gatgatcttg gcgaagacat
     5041 tgtcgggatc gtaagtcatc gttgctccgg tgttactccg gtctccggtt cagacgtcga
     5101 gcatattcga aaaggcgcgt catcgggcac cggtggtaat ctgtcgtttt gcgcgggggg
     5161 aatgcgatgc agctaaaagg ccaggtcgcc gtggtgacgg gcggcgccag cggcatgggc
     5221 cgcgacacgg tcctgcgttt tcttgccgag ggcgcacgcg tggtcatcgc ggacttcaac
     5281 gcccggacgg gccaggccgt tctcgaagag gcgcgcgccg ccggattcgg cgagtcgacg
     5341 cgattcgttc gcaccgacgt ggcgcgggag gcggacgtgg aggccatgtg cggtctggct
     5401 gtggaagcct tcggccggct cgacgtcgtg ttcaacaacg cc
//
LOCUS       DBNHOX010000162        11439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_12761, whole genome shotgun sequence.
ACCESSION   DBNHOX010000162 DBNHOX010000000
VERSION     DBNHOX010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11439
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_12761"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..563
                     /locus_tag="AC16UZ_03465"
     CDS             213..563
                     /locus_tag="AC16UZ_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245771.1"
                     /translation="MRTVRHRARSTKDGRRVGLAFAGLLATAFFASCASYSAGSLNQI
                     HSELVWERRIECRKAWSAYESACRGNLLMYQGMTEMSFCQAWAKPRTVNGLMVWPPHQ
                     LLPQSVDRHGRPCR"
     gene            715..2481
                     /gene="xsc"
                     /locus_tag="AC16UZ_03470"
     CDS             715..2481
                     /gene="xsc"
                     /locus_tag="AC16UZ_03470"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF005713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HIR6245772.1"
                     /translation="MKMTSEEAFVKVLQRHGIDNAFGIIGSAFMPVSDLFTRAGITFW
                     DCAHEGSGGMMADGFTRASGRMSMMIAQNGPGITNFVTAVKTAYWNHTPVLLVTPQAA
                     NQTIGQGGFQEVEQMKLFEDMVCYQEEVRSASRMAEVLNRVILQAKRHSAPAQINVPR
                     DLFTQVIDIELPPIIDFERPSGGTRAIDEAAALLSKAEFPVILNGAGVILADAIGDSA
                     ALAERLEAPVCCGYQHNDAFPGSHPLFAGPLGYNGSKAGMELIAHADVVLALGTRLNP
                     FSTLPGYGIDYWPKDAAIIQVDINPSRIGLTKPLSVGIVGDAGDVARALLERVEEVSD
                     EERDARRNLIARTKANWQQELAGMDHEDDDPGTTWNERARADKPDWMSPRMAWRAIRS
                     VLPTEAIISSDIGNNCAIGNAYPTFETGRKYLAPGLFGPCGYGLPSIIGAKIGCPDVP
                     VVGFAGDGAFGISVTELTAIGREEWPAITMIVFRNYQWGAEKRNSTLWFDDNFVGTEL
                     DEQVSYAGIAQACGLLGVQARTMDELSAALGEALDNQMNHGRSTLIEAMINQELGEPF
                     RRDAMKKPVEVAGIDPADMRRA"
     gene            complement(2521..3105)
                     /locus_tag="AC16UZ_03475"
     CDS             complement(2521..3105)
                     /locus_tag="AC16UZ_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245773.1"
                     /translation="MGVFEYVAGFVAIVVGLAVARVMGGIGAFILSDRRAAMDWLLAG
                     WCLALIVTLIGWWMLAWFTLGQLETITYGAVFGWFAATALLYLASYILVPAGGAAFGG
                     AGAALGELRPAFFVCLAVHFAANIVESAVTGSLLPAQGLVVLMVVLSGIGAFLRGARA
                     HALLLLTWMVCMLVLNFVAVPAVGVNSGSVLEAG"
     gene            3251..3955
                     /locus_tag="AC16UZ_03480"
     CDS             3251..3955
                     /locus_tag="AC16UZ_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013223.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriorhodopsin"
                     /protein_id="HIR6245774.1"
                     /translation="MDKLFLQSGDFVGVTFWLVSIAMIAATVFFLYEGVKVKPQWRLS
                     LLVAGLVTLVAGVHYDYMRDYWVVEQATPIVYRYIDWLITVPLLMIEFYIILRAAGAA
                     LGHGSFVRLLVGTLVMLIFGYLGEAQVLNIHVGFWIGMVGWAIIIYEIFGGAAGKAAS
                     ASAQAVQSAFSAMRLIVLVGWAIYPIGYIWGYMLGGVDEGALNMVYNLADFVNKILFG
                     LIIWNLAIKDSGEAGH"
     gene            complement(4017..4538)
                     /locus_tag="AC16UZ_03485"
     CDS             complement(4017..4538)
                     /locus_tag="AC16UZ_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245775.1"
                     /translation="MSIVELGAIGEFLGAFAVVITLVYLGLQVRHNTTSTRAGTLAYA
                     TDAWSDYLQRQSVDDLNLLLGLSIEPDELSSAEFMRAYYLYRVMFRRMESDFYQYRSG
                     ILDADTWDAYVESWRGDVFITPGGRAMWKLQRDYFGPEFRSAFDDVEASTPRTPQHLR
                     RRFAELWEAERPV"
     gene            4694..5206
                     /locus_tag="AC16UZ_03490"
     CDS             4694..5206
                     /locus_tag="AC16UZ_03490"
                     /inference="COORDINATES: protein motif:HMM:NF016162.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HIR6245776.1"
                     /translation="MWKFTGKSRPEFAEEPGPGQESVWDYPRPPALDPSDEQVEVRAD
                     GTPIASSRRTLRVLETAGPPTYYIPEQDIDWSQLTEIAQTTYCEWKGHASYYALASDP
                     ARVPVAWLYADPSDSFRAIDRHASFYPGRVECLVNGERVRPQPGEFYGGWITDRVVGP
                     FKGEPGTGHW"
     gene            complement(5222..6196)
                     /gene="egtD"
                     /locus_tag="AC16UZ_03495"
     CDS             complement(5222..6196)
                     /gene="egtD"
                     /locus_tag="AC16UZ_03495"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052706 - L-histidine
                     N(alpha)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HIR6245777.1"
                     /translation="MKQARRKARNVMFRDEHPDLGDSRREILAGLQRDQKAVNPKWFY
                     DERGSQLFDQITRLPEYYPTRTEVGILQENRREISACCGSGCLFIEPGSGSSEKVRIL
                     LETLKPSAYVPVDISAGFLEEAALKLGDEYPWLDIYAVCADFNRSWSYVDDLPEGKRV
                     VFYPGSTIGNLEPEAAVGFLRRVREITGDSGGVVIGVDTHKSTERLNAAYNDAAGVTA
                     DFNLNVLRRMNDLLDAEFDEGSFRHHAFYNTELKRIEMHLVSERAQAVRCNGATVEFA
                     EGETIHTENSYKYTVEDFAELVDAAGLELQQSWTDADELFSVHYLRVA"
     gene            complement(6193..7467)
                     /gene="egtB"
                     /locus_tag="AC16UZ_03500"
     CDS             complement(6193..7467)
                     /gene="egtB"
                     /locus_tag="AC16UZ_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HIR6245778.1"
                     /translation="MGKALAIDATAAAFSDTRRQSVDVCRPLEIEDYGLQAAVFASPP
                     KWHLAHTTWFFETFILKPFVSGYAPVEPIYEVLFNSYYNGIGEQHPRHQRGLLSRPTL
                     DEVLAYREHVDAAMAGCLELAGRADPQAIRQRTVLGIEHERQHQELFFTDLKYSLSVN
                     PLYPAYLAAELPVQSGTPVRWLGCEGGLVEIGHDGSGFCFDNELPRHRCFVEPFEIAS
                     RLVTNEEYQAFVDDDGYGRPELWLADGWTTLQSEGWKAPLHWLERDGQALEFTLHGLQ
                     PRRGSRPVCHVSGYEADAFARWANARLPTEQEWETVAAGHPAGAAGMDDGVYHPQAAP
                     ADEGPTQLYGACWQWTRSAYGPYPGYSASPGAIGEYNGKFMSNQWVLRGGSCVTKSSQ
                     ARPTYRNFFYPQDRWQFSGIRLARSVEGERRL"
     gene            complement(7609..9084)
                     /locus_tag="AC16UZ_03505"
     CDS             complement(7609..9084)
                     /locus_tag="AC16UZ_03505"
                     /inference="COORDINATES: protein motif:HMM:NF015995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR6245779.1"
                     /translation="MKGACLLLAAACIVLVAPVRAAERVTVASKAFSESYVLAEIAAQ
                     LLEAEGFEVERKLGLGGTLIAFGALREGAVDVYPEYTGTLTQAILDEPGMTIAELDAA
                     LAERGLVFRVPLGFNNSYAVAVTEEMAGARGLTRVSDLAGQTDLSFGFSHEFLNRADG
                     WPALKTAYGLTQTASGIEHALAYRALASGHLDVTDAYTTDGELSLYALRLLADDRGFF
                     PTYDAGLLTRDDMPAPVAAVLSRLESRIDEETMQGLNRRVASQGEEPARVAADYLRET
                     GLVSAADAVAVAGYERILGNTLVHLKLTGIALLLGCLVAVPLALVVARHQRIASGLLY
                     VTGLVQTVPSLALLALLIPLVGLGQLPAIIALFLYSLLPIVRNTLTGLFNVDPLLKEV
                     AGSMGLTTAQQLRKVELPLAMPTILAGIKTAAIISIGTATLAAFVGAGGLGEPIITGL
                     TLNDHALILEGAVPAALLAVVVELLFELIERATVPAHLRVS"
     gene            complement(9081..9821)
                     /locus_tag="AC16UZ_03510"
     CDS             complement(9081..9821)
                     /locus_tag="AC16UZ_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6245780.1"
                     /translation="MPVLSLEDVTKAYGGRPALENVDLALDSGQVTAIVGRSGCGKST
                     LLKLLNGLVVPDAGRVSAFGTQLDYADLVSVRRRMGYAVQGTGLFPHMSARENVVLSA
                     EIAGWKEQAVAQRLGELLELTRLSAELLAKYPHELSGGQQQRVGLCRAMMLRPEVLLL
                     DEPFAAIDPITRYDIHHQLLEILAAEPATVVLVTHDMREAMQLADRIVLLEGGRVFLD
                     ESRDSLARSRPGVEPEALLQSLMEEALA"
     gene            complement(9924..10472)
                     /locus_tag="AC16UZ_03515"
     CDS             complement(9924..10472)
                     /locus_tag="AC16UZ_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245781.1"
                     /translation="MRSAMTRIAALVVLFAPIASLQADHHGALIAQARSAAPELVSAD
                     ATVMYRGEVLVEGTNGWVCMPETLPDDNSPMCNDAVWMGLMQAMDAGEEYEVERIGIS
                     YMLQGDAGVSNSNPAHADHVNAPDFIKEGAHLMIAVPRAMLEGITDDPHGGGPYVMWK
                     DTPYAHIMVPLEDANRSAPPAQ"
     gene            10637..11038
                     /locus_tag="AC16UZ_03520"
     CDS             10637..11038
                     /locus_tag="AC16UZ_03520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6245782.1"
                     /translation="MATFLAKIKIFEGKEAEFEETARMMFRETHANEPTCRRYEYWRG
                     AEPRTYYCMESFDDFVGFMEHQVSPHHEAPDFASMLEEIELEWIDPIQGACDLTSTNP
                     KDLPEDASDLMKEYAATHPLVMQDWWLNLRK"
     gene            11051..>11439
                     /locus_tag="AC16UZ_03525"
     CDS             11051..>11439
                     /locus_tag="AC16UZ_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR6245783.1"
                     /translation="MKTRAAVAFEPKKPLEIVEVDLEGPRTGEVLIEIKATGICHTDA
                     YTLDGLDSEGVFPSILGHEGAGVVVETGPDVTSVAPGDHVIPLYTPECRQCKSCLSRK
                     TNLCTAIRATQGQGLMPDGTSRFSFEG"
BASE COUNT         2134 a         3815 c         3578 g         1912 t
ORIGIN      
        1 agcggtaccg gcagctgacg gaattcaggc ttgcacacgc ctgaacggtc ggaaagtttc
       61 ccggtttgtg acgcgtttgg catttgcgtt ggctcgctcg ttattctctg cagtctctct
      121 ccatcattgg ctcctaacat ctgaatcgac ccggtagatc ttcatcgctg ataggggtcg
      181 ccgggctgtc ctcctccaaa gcgaggtagc agatgcggac agtcagacac agagctcgga
      241 gcaccaagga tggtcgacgg gttggtctgg ccttcgcggg actgctcgcc accgccttct
      301 tcgccagctg tgcctcctac tccgccggaa gcctcaacca gattcacagc gaactcgtct
      361 gggagcggcg gatcgagtgc agaaaggcat ggtccgccta cgagtcggcc tgccgcggca
      421 atctgctcat gtatcagggc atgacggaga tgagcttctg ccaggcttgg gcaaagccgc
      481 gcaccgtcaa tgggctcatg gtgtggccgc cacaccaact gctcccccaa tcggtcgaca
      541 ggcacggccg cccctgccgg taagcgacga ccggactttc ggcaacccgc tggaaaagtc
      601 cgtggtcccg tgttaaacac gcccgctggc tctgcggaca ccgagccgaa cgggacttga
      661 atcaccatcg agcagctgct cgcgcgacgg cgcgggcgtg gagatttgtc gaccatgaaa
      721 atgacgtctg aagaagcctt cgtgaaagtc ctgcaacggc acggcatcga caacgcgttc
      781 ggcatcatcg gatccgcctt catgcccgtc tcggatctct tcacccgggc cgggattacc
      841 ttctgggact gcgcgcacga aggatccggc ggcatgatgg ccgacgggtt cacccgggcc
      901 tcgggccgga tgagcatgat gatcgcccag aacggtcccg gcattacgaa cttcgtgacc
      961 gcggtcaaga ccgcctactg gaaccacacc cctgtgctcc tggtgacccc tcaggccgcc
     1021 aaccagacca tcggtcaggg cgggttccag gaagtggagc agatgaagct gttcgaggac
     1081 atggtctgct accaggagga ggtgcgctcc gcctcccgca tggccgaggt gctcaaccgc
     1141 gtgatcctgc aggccaagcg acactcggca cccgcccaga tcaacgtgcc gcgggacctg
     1201 ttcacccagg tgatcgacat cgagctgccg ccgatcatcg acttcgagcg cccgagcggc
     1261 ggtacgcggg ccattgacga ggccgccgcc ctgctgtcga aagccgagtt ccccgtcatc
     1321 ctgaacggcg ccggcgtgat ccttgccgat gccatcggcg attccgccgc cctggccgag
     1381 cgcctggagg ctcccgtgtg ctgtggatac cagcacaacg atgcctttcc cggctcgcac
     1441 cccctgttcg cgggcccgct ggggtacaac ggatccaaag ccgggatgga gctcattgcc
     1501 cacgccgacg tggtgctcgc cctggggacg aggctcaacc cgttctcgac cctgcccggc
     1561 tacggcatcg actactggcc gaaggatgcc gccatcattc aggtcgacat caatccgtca
     1621 cgcatcggcc tcaccaagcc cctgagcgta ggcatcgtgg gtgatgcggg cgacgtcgcc
     1681 cgggccctgc tggaacgcgt ggaggaggtg agcgacgaag agcgtgacgc gcggcgcaac
     1741 ctgatcgcca gaaccaaggc caactggcag caggagctgg ccggcatgga tcacgaggac
     1801 gacgatccgg gaaccacctg gaacgagcgc gcgcgcgccg acaagccgga ctggatgagc
     1861 ccccgcatgg cctggcgggc gatacgcagc gtgctgccca cggaagccat catcagctcg
     1921 gacatcggca acaactgcgc catcggcaac gcctacccca ccttcgagac cggcagaaag
     1981 tatcttgcgc ccggcctgtt cggcccctgc ggctacggcc tgccttcgat catcggcgcg
     2041 aagatcggct gccccgacgt gccggtggtg ggcttcgccg gcgacggcgc cttcggcatc
     2101 tcggtcaccg agctcaccgc catcggccgc gaggagtggc cggccatcac catgatcgtc
     2161 ttccgcaact accagtgggg ggcggagaag cgcaactcca cgctgtggtt cgacgacaac
     2221 ttcgtcggca cggagctcga cgagcaggtg agctacgccg gcatcgccca ggcctgcggg
     2281 ctgctcggag tccaagccag aaccatggac gagctttccg ccgctctcgg tgaagcgctg
     2341 gacaaccaga tgaaccacgg caggtcgacg ctcatcgagg ccatgatcaa ccaggagctc
     2401 ggcgagccct tccggcgcga cgccatgaag aagccggttg aggtcgcagg catcgatccc
     2461 gcggacatgc gacgcgctta ggctggaatc ggggctggcc gggcagcggg cgcgttcggc
     2521 tcagcctgct tccagcaccg agcccgagtt tacccccacg gccgggaccg caacgaagtt
     2581 caggacaagc atgcagacca tccaggtcag cagcagcagg gcgtgtgccc gggcgccgcg
     2641 aagaaacgcg ccgattcccg agagcacgac catcaacacg accagcccct gagccggcaa
     2701 gagagaacct gtaacagcgg actcgacgat gttggcagca aagtgcacgg ccaggcagac
     2761 gaagaaggcg ggccgcaatt cgcccagggc cgcaccggct ccgccgaaag ccgctcctcc
     2821 agcgggaacc agaatgtagc tcgcgagata gagcagcgcc gtcgccgcga accaaccaaa
     2881 tacggcgccg taggtgatgg tctccagctg ccccagggtg aaccaggcca gcatccacca
     2941 tccgatcagc gtgacgatga gcgccaggca ccagccggcc aggagccagt ccatcgccgc
     3001 gcgcctatcc gacagaatga aagcgccgat cccacccatg acccgcgcga cggcgaggcc
     3061 gacgacgatg gcaacgaacc cggctacgta ttcgaacacg cccatggcaa ctccccgtcg
     3121 cacaccctgg cctttcccga tccagcatca ccagtgatga cagacccgtt cacgcagctt
     3181 tcacgtgagc cctgttgaca tccacgatgc agtaaaactc aagcaataac gcaatttaca
     3241 gggattaaca atggacaagc tctttctgca gagcggcgac ttcgtaggcg tcaccttctg
     3301 gctggtgtct atcgcgatga tcgctgccac cgtcttcttc ctttacgagg gcgtcaaggt
     3361 caaaccgcag tggcggctct cacttctggt cgccggcctc gtgactctgg tcgccggcgt
     3421 tcactacgac tacatgcgcg actactgggt cgtcgagcag gcgacgccca tcgtctaccg
     3481 ctacatcgac tggctgatca ccgttccgct gctgatgatc gagttctaca tcatcctgcg
     3541 cgcggcgggt gcagccctgg gacacgggtc gttcgtgcgg cttctcgtcg gcacgctggt
     3601 gatgctcatc ttcggctatc tcggcgaagc ccaggtgctc aacattcacg tcggcttctg
     3661 gatcggcatg gtcgggtggg ccatcatcat ctacgagata ttcggcggag ctgccggcaa
     3721 agcggcatct gcctcggcgc aggcggtgca gtccgctttc agcgccatgc gcctgatcgt
     3781 gctggttgga tgggccatct acccgatcgg ctacatatgg ggctacatgc tcggcggcgt
     3841 ggacgaaggc gcgctgaaca tggtgtacaa cctggccgac ttcgtgaaca agatcctgtt
     3901 cggtctgatc atctggaacc tcgccatcaa ggactcgggc gaagccggac actgagttag
     3961 cccagccacc tgagggcccc acccggggtg gggcccgttc ggctgagagc agaaggtcaa
     4021 accggccgct cagcctccca caactccgcg aaccggcggc gcaggtgctg aggcgtgcgg
     4081 ggcgtgctcg cttcgacgtc atcgaaagcg cttcgaaact ccggtccaaa ataatcgcgc
     4141 tgcaatttcc acatggcgcg tccgcccggc gtgatgaaca cgtcgccgcg ccagctctcc
     4201 acgtaagcat cccatgtgtc cgcatcgagg attccgctgc gatactgata gaagtcgctt
     4261 tccatccgcc ggaacatcac gcgatacaga tagtaggcgc gcatgaactc cgcgctggac
     4321 agctcgtcgg gctcgatcga aagccccagc agcaggttca gatcgtccac cgactgtctc
     4381 tgcaggtagt cgctccaggc gtccgtcgcg taggcgagcg tgcccgcccg ggtcgacgtg
     4441 gtgttgtgac ggacctgcag acccaggtag accagagtga tgaccacggc aaacgcgccc
     4501 agaaactccc caatcgcgcc aagttctacg atgctcatga atggctcctc aattacggca
     4561 tgccaagcgt aacgaagctc aggcgtttgg ccgagtcccg atctccaggg aagcacggca
     4621 tcgaccacga accgccgtcg acgggctagc gccccatgcc ggccgggttg aacacctgtt
     4681 agtatcctcg gccatgtgga aatttacggg aaagagcaga ccggagtttg ccgaggaacc
     4741 gggacccggc caggagtccg tctgggacta ccctcgccca ccagcgctgg acccgagcga
     4801 cgagcaggtc gaggtgaggg ctgacgggac gccgatcgca tcaagccgca ggacgctgcg
     4861 ggttctggag acggcggggc cgcccaccta ttacatccca gagcaagaca tcgactggag
     4921 tcagctgacg gagatcgccc aaaccaccta ctgcgagtgg aagggtcacg ccagctatta
     4981 cgctctcgca tccgaccccg ccagggtgcc cgtcgcgtgg ctttacgccg acccttccga
     5041 ctccttccgg gccatcgacc ggcacgcctc gttctatccg gggcgggtcg agtgcttggt
     5101 caacggcgag cgggtaaggc cccagcccgg ggagttctac gggggctgga tcaccgaccg
     5161 cgtggtcggc ccgttcaagg gagaacccgg caccgggcac tggtagacct cgggcttcgg
     5221 gtcaggccac tctcaggtag tgaacgctga agagctcgtc ggcgtccgtc cagctctgct
     5281 gcagctccag gccggccgcg tccacgagct ccgcaaagtc ctccacggtg tacttgtacg
     5341 agttctccgt gtggatggtc tctccctcag caaactcgac tgtggcaccg ttgcagcgca
     5401 cggcttgggc ccgctcgctc accaggtgca tctcgatccg cttcagctcg gtgttgtaga
     5461 acgcatggtg gcgaaagctc ccctcgtcga actcggcatc gagcaggtcg ttcatccgcc
     5521 gcagcacgtt caggttgaag tccgccgtga cgccggccgc atcgttgtac gccgcgttca
     5581 gtcgttcggt tgacttgtgc gtgtccacac cgatgacgac gccgcccgaa tcgcccgtga
     5641 tctcgcgaac ccgacgcaga aaccccaccg ccgcctcggg ttcaaggttg ccgatggtcg
     5701 acccagggta gaaaaccacc cgtttacctt ccggcaggtc gtccacgtag gaccagctcc
     5761 gattgaaatc cgcgcagacc gcgtagatgt ccagccaggg gtactcgtcg ccgagcttca
     5821 aggcggcttc ctcgaggaaa ccggcggaga tgtccacggg gacgtaggct gacggtttga
     5881 gagtctcgag caggatcctg accttctcgc tggaaccgct cccaggctcg atgaacagac
     5941 aaccgctgcc gcagcaggcg ctgatctcgc gacgattctc ctgcaggatc cccacctccg
     6001 tccgggtcgg gtagtactcg ggcaggcggg taatctgatc gaacagctgg gagccccgct
     6061 cgtcgtagaa ccacttcggg ttgaccgctt tctgatctct ctggagaccg gccaatatct
     6121 cgcggcggct gtcgccgaga tccggatgct cgtcccggaa catgacgttt cgagcctttc
     6181 tgcgcgcctg cttcataacc gtcgttcgcc ctccacggag cgggccaggc ggatgccgct
     6241 gaactgccag cgatcctggg gatagaaaaa attgcggtaa gttggcctcg cctggctgga
     6301 cttggtcacg caggagcctc ctcgcagaac ccactgattg ctcatgaact tgccgttgta
     6361 ctcgccgatg gcgccgggcg aagcgctgta acccgggtac ggaccgtacg cgctccgcgt
     6421 ccactgccag cacgcgccgt acagctgcgt cgggccttca tctgcaggag ctgcctgagg
     6481 gtggtagacg ccgtcgtcca tgcccgccgc tccggcggga tgtccggccg ccaccgtttc
     6541 ccactcctgc tcggtcggca gccgcgcgtt cgcccagcgg gcgaaagcat cggcctcgta
     6601 gccgctcaca tgacagacgg gccgcgaccc ccgcctcggc tgcaggccgt gcagggtgaa
     6661 ctccagcgcc tggccgtcac gttcgagcca atgcagcggc gctttccatc cttcactctg
     6721 caacgtcgtc cagccgtcgg ccagccacag ctccggacgc ccgtagccgt cgtcgtccac
     6781 gaacgcctgg tactcctcgt tggtgacgag cctggaggcg atctcgaaag gctccacgaa
     6841 acatcgatgt cgaggcagct cgttgtcgaa gcagaagccg cttccgtcat ggccgatctc
     6901 gacgaggccg ccctcgcagc caagccaccg caccggcgtc ccgctctgca ccggcagctc
     6961 ggcggcgagg taggccgggt acagcgggtt gacgctcagc gagtatttga ggtccgtgaa
     7021 gaagagctcc tggtgctggc gctcgtgctc gatgcccaga acggtgcgct ggcgtatggc
     7081 ctgcggatcg gcccgtccgg ccagttccag acagcctgcc atcgctgcgt ccacgtgctc
     7141 ccggtaggcg agcacctcat ccagcgtggg gcgcgatagc aacccccgct gatgccgcgg
     7201 atgctgctcg ccgatcccgt tgtagtaaga gttgaagagc acctcgtaga tgggctcgac
     7261 gggcgcgtag cccgagacga acggcttgag gatgaaggtc tcgaaaaacc aggtggtatg
     7321 agcgagatgc cacttcggag ggcttgcgaa cacggcggcc tgcaggccgt agtcctcgat
     7381 ctccaacggc cggcagacgt ccacggattg tcgccgggta tcgctgaacg cggccgccgt
     7441 cgcgtcgatt gccaaagcct ttcccatatt gatcgtctgc ccctcccggc ccgcagatcc
     7501 gagccgcgcg ccgctgagcg tgcgccattc cggggggtca ggccataggc ccgccgcctc
     7561 cgactcctct aaagcctaac agcttcggac cggactttga cgacatcgtc aggaaacccg
     7621 cagatgtgcg ggaaccgtcg cccgctcgat gagctcgaag agcagctcca cgacgacggc
     7681 cagcaaagcg gcaggaaccg cgccttccag aatcagcgcg tgatcgttca gcgtgagacc
     7741 ggtgatgatc ggctcgccga gcccgccggc gcctacgaag gcggccaggg tggccgtccc
     7801 gatgctgatg atcgcagcgg tcttgatgcc cgcgaggatg gtgggcatgg ccaggggcag
     7861 ctccaccttc ctgagctgct gcgccgtggt cagccccatg ctcccggcca cctccttgag
     7921 cagcgggtcg acgttgaaca gcccggtgag cgtgttgcgc acgatgggca gcagggagta
     7981 gagaaacagc gcgatgatgg ccggcagctg gcccaggcca acgagcggga tgagcaacgc
     8041 cagcagcgca agggaaggca cggtctgcac caggcccgtc acgtacagca ggccgctggc
     8101 gatgcgctgg tgccgcgcga cgacaagggc caggggcacg gccacgaggc aacccaaaag
     8161 cagcgcaatg ccggtgagct tcaggtgaac gagggtgttg cccagtatcc gctcgtaccc
     8221 ggccacggcc acggcatcag ccgccgacac cagccccgtt tcccggaggt agtcggccgc
     8281 tacgcgggca ggctcctcgc cctggctggc cacgcgccgg ttcagcccct gcatggtctc
     8341 ctcgtcgata cggctttcaa gccgcgacag cacggcggcc acgggcgcgg gcatgtcgtc
     8401 acgggtcagc agcccggcgt cgtaggtggg aaagaagccg cggtcgtctg ccagcaggcg
     8461 aagcgcgtag agcgaaagct ccccatccgt ggtgtaggca tcggtcacgt cgagatgccc
     8521 gctcgccagg gcgcggtagg ccagggcgtg ctcgattccg gatgccgtct gggtcaggcc
     8581 gtaggccgtc ttgagcgcag gccagccgtc ggcgcgattg agaaactcgt gggagaagcc
     8641 gaagctgaga tccgtctgcc cggccaggtc gctcacccgc gttaagcccc gggcaccggc
     8701 catctcttcg gtgacggcca cggcgtagct gttgttgaac ccgagaggga cccggaaaac
     8761 gagcccgcgc tcggccagag cggcgtcgag ctccgcaatg gtcatgcccg gctcgtcgag
     8821 tatcgcctgg gtcagggtac ccgtgtactc gggatatacg tccacggcgc cctcccgaag
     8881 cgcgccgaag gcaatcagcg tgccgccaag gcccagcttg cgttccacct cgaagccttc
     8941 cgcctccagc agctgggcgg cgatctccgc gagcacgtag ctttcgctga acgctttgga
     9001 tgccacggtg acccgctcgg ccgctcgcac aggtgccaca aggacgatgc aggcagccgc
     9061 gaggagcagg catgctccct tcatgccagc gcctcctcca tcagcgactg cagcaaagcc
     9121 tcgggctcca cgcccggcct ggagcgggca agggagtccc gggactcatc gagaaacacc
     9181 cggcctcctt ccagcaggac gatgcggtcc gccagctgca tggcttcccg catgtcgtgg
     9241 gtgaccagaa cgacggtggc gggctccgcg gcgagaatct ccagcagctg atggtggatg
     9301 tcgtagcggg tgatgggatc gatggccgca aacggctcgt ccagcaggag gacctcgggc
     9361 ctgagcatca tggcccggca caggcccacg cgctgctgct gcccgccact gagctcgtgg
     9421 gggtacttgg cgaggagctc agcggaaagg cgcgtaagct cgagcagctc gccaagccgt
     9481 tgagccaccg cctgctcttt ccagcccgca atctccgccg agagaacgac gttttccctg
     9541 gcactcatgt ggggaaagag gcccgtgccc tgcacggcgt aacccatccg ccggcgcacg
     9601 gataccagat cagcgtaatc cagctgggtt ccgaacgcgg agactcggcc tgcgtcgggc
     9661 accaccagcc cgttcaggag cttcaacaag gtggacttgc cgcacccgct gcggccgacg
     9721 atggccgtca cctggccgga gtccagcgcg agatctacgt tctcgagcgc cggacgcccg
     9781 ccataggctt tggtcacatc ctcgagcgac agcactggca tgacgctatc taaacatgtc
     9841 gaccacgtcg gatgaaaatc tcaacggcaa tggacgccgg ccggggccgg catcagcccc
     9901 gccgggcgct ggcaccgggc gtcctattgc gccgggggag ccgaccggtt tgcgtcctcc
     9961 agcggcacca tgatgtgcgc gtagggcgtg tccttccaca tcacgtaggg cccacccccg
    10021 tgcgggtcat cggtaatgcc ctcgagcatc gcgcgcggga cggcaatcat gagatgcgcg
    10081 ccttccttga tgaaatcagg ggcattcaca tggtcggcat gcgcagggtt cgagttgctc
    10141 acgccagcat ctccctggag catgtaggag atgccgatgc gctccacctc gtattcttca
    10201 ccagcgtcca ttgcctgcat caggcccatc cagaccgcat cgttgcacat gggcgagttg
    10261 tcgtccggca gcgtctccgg catgcagacc cagccgttgg tcccttcgac cagcacctcg
    10321 ccgcgataca tcacagtcgc atcggcgctc accagttcgg gggccgccga ccgggcctgg
    10381 gcgatgagcg ccccgtggtg gtccgcctgc agcgacgcga tcggcgcaaa caacacaacc
    10441 agggcagcga tccgggtcat agctgatctc atcatctgtc tccttggaaa atcgacgctc
    10501 cagacgttac gcccggcggc accctggggc aaggcgaatc acccagttgc ccatgtcccc
    10561 ggtcgccaaa cgaggaaggg tgcggttatc cttgctagac cgataaatca tcatccaggg
    10621 agcatgaggg gggaccatgg ccacgtttct ggcaaagatt aaaattttcg agggcaagga
    10681 ggctgagttc gaggagaccg cgcggatgat gttccgcgag acccatgcca acgagcccac
    10741 ctgccgacgt tacgagtact ggcggggcgc ggagccgcgc acgtactact gcatggagtc
    10801 gttcgacgac ttcgtcggct tcatggagca tcaggtttcg ccccaccacg aagcaccgga
    10861 cttcgcctcg atgctcgagg agatcgagct ggagtggatc gatcccatcc agggcgcctg
    10921 cgacctgacg tccaccaatc ccaaagacct gcccgaggat gccagcgatc tcatgaaaga
    10981 gtatgcggca acccaccccc tggtcatgca ggactggtgg ctgaacctcc gcaagtaaca
    11041 gggagcaaat atgaagacgc gtgcggccgt ggccttcgag cccaagaaac cgctggagat
    11101 cgtggaggtc gacctggagg gtcccagaac cggcgaggtg ctcatcgaga tcaaggccac
    11161 cggcatctgc cacaccgacg cctacaccct cgacgggctc gattccgaag gggtctttcc
    11221 gtccatcctc ggccacgagg gcgccggcgt cgtggtcgag acaggcccgg atgtcacttc
    11281 ggttgcgccc ggcgatcacg tcattcccct gtacacgccg gagtgccggc agtgcaaatc
    11341 ctgtctgtcc cgaaagacga atctgtgcac ggccatccgc gccactcagg gccaaggcct
    11401 gatgcccgac ggcacctccc gattctcgtt cgagggcaa
//
LOCUS       DBNHOX010000163         3066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1106085, whole genome shotgun sequence.
ACCESSION   DBNHOX010000163 DBNHOX010000000
VERSION     DBNHOX010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1106085"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="AC16UZ_03530"
     CDS             <1..667
                     /locus_tag="AC16UZ_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014068.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HIR6245784.1"
                     /translation="ESPVLCPHGGGTHEPRELSVPYYAFLDAGLEVDIASPQGGRVPV
                     EPNSMGWPLATDADRRFKRDSTAMRQLAQSLRVGDLDPATYDVVFLVGGWGAAYDLGQ
                     SSELGEFMTQANANGAVIGGVCHGPLGLLQATDSDGRPLVEGRRLTAVSDVQVQQLGI
                     EITPLHPETELRELGALYEKDTAFRDFFATHVVVDGNLVTGQNQNSGAETAHRMMEIL
                     AGR"
     gene            1929..>3066
                     /gene="nhaA"
                     /locus_tag="AC16UZ_03535"
     CDS             1929..>3066
                     /gene="nhaA"
                     /locus_tag="AC16UZ_03535"
                     /inference="COORDINATES: protein motif:HMM:NF018648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HIR6245785.1"
                     /translation="MVAGDRSGTDLPTVVVDRFTGPLARFLKVEAATGVLLLLAAVAA
                     LGLANSPWSVSFLAFWETPIGLHFGPFDFSRSLRHWINDGLMTFFFFVIALELKRALI
                     LGELRDLRVASLPFAGALGGMIVPASLYFVLMSGRPGANGWGTVMATDTAFVIGCLAL
                     FGSRIPASLRVFAVSLAVFDDVGAILIVAAVYGGALNLNALTLGMLGVVAVAVCARLG
                     MRSIPLYFLLGGAVWLCFDASGIHATMAGVVLGLMTPTRVWVSDARLRGILGRALSHP
                     EGEHWSGDSVERSDLLRAGTAVRESLSPLERLEMMLHPWVGFLIMPLFALANAGIAIS
                     GADIGQPVSVAIFAALVFGKPVGVLFFSGIAVRLGFAIRPLGLDW"
BASE COUNT          474 a          976 c         1022 g          594 t
ORIGIN      
        1 tgagtcgcct gtgctctgcc cgcatggcgg cggtactcat gagccgcggg agttgtccgt
       61 gccctactac gccttcctgg acgccgggct cgaggtcgac atcgccagcc ctcaaggggg
      121 tcgcgtcccc gtcgagccga actcgatggg gtggcctctg gcgacggacg cggatcgccg
      181 gttcaagcgg gactcgacgg ccatgcgcca gcttgcgcag tccttgcgcg tcggcgacct
      241 cgaccctgcg acgtacgacg tcgtcttcct cgtgggaggc tggggcgccg cctacgatct
      301 aggacagagc tcggagctgg gtgagttcat gacccaggcg aacgcgaacg gcgccgtgat
      361 cggaggcgtc tgccacgggc cgctggggct ccttcaggcc acggactcgg atggacggcc
      421 actggtcgag ggacgcagac tcaccgccgt gagcgatgtc caggtccagc agctcggcat
      481 cgagatcacg ccgctgcacc ccgagacgga gctacgcgag ctcggcgcgc tgtacgagaa
      541 ggacacggct tttcgcgact tcttcgcgac gcacgtggtg gtcgacggca acctcgtgac
      601 cggccagaac cagaacagcg gggccgagac cgcgcaccgt atgatggaga ttcttgcagg
      661 gcgatgaggc acgtcgcggc cacgctcctc gtcctgactg ctaggggaaa tctcccacaa
      721 cgaccgtcga acacccgccg atactgcggt cgctcgaccc gggtatctta aagatgcgcc
      781 tgacgtggcg ccccagcgat ttgaggcgga ttgcagctgg gagatcaatc tgctaaaccc
      841 gccgggggtc ccgagcggag ggaatagccc gggaccccgc agttttctta ccccccgttc
      901 gcgagcgctg gtggggcgag ggctcagccg atcaggcggg cgcttcggac ctgcggtatc
      961 gtgagctcag gtgccccgac ctgcgtcaga accggccggg gcactccgtc acgtaccaga
     1021 cggagcagcg aatcggcaca cgttgctgtt gagtcccgac ggcgtcgtca cgaagtcggc
     1081 attcagggtc tctccgacag catccccgtc gtaggcgcag gagaggcacc tatacccagg
     1141 gtggcggtga cttcagctac actgcggctg gaatcaccta ccggggctcg gtacacgggt
     1201 cgttcacgat cgcgatcgac gagtcggatc tgacgttcgc gacaaccgcc gatccggccc
     1261 gatggcgcac gtacacgaga cccgggcgct gagtgggagt cgtcgggcgg agtcttctcc
     1321 ttgcgtcgtg cggtcacctg caacggaacc tgcaccatcg cctacgggcg ccggccatcg
     1381 ctgacgagcg catcgtacga gagaacctgg agtttccggc gtccctcggc gctgaccata
     1441 gccccgacga atcctgatga gcgtggggtc ggtagttcac ctggagcggg cagagcccgc
     1501 ggaggggagc gagggccggt atgacggtcg cgaagcgccg gtcaggtaag cccgcagctg
     1561 aaatctaccc aggcccactg agggaagggg aagccccgct tggtcttagg tggccgggcg
     1621 gggtttcccc gttcctactc acgaggaagt ctccgttgtt ctatccgttc gcgcccggaa
     1681 cgggacgcca gcgccttcct tcgcacgccc tgatctgggt gctgatagaa cttcagaatg
     1741 gtcgcgggct ccttccagcc acccagtggt tcatgaggtc ggaagcgaac ttccgacagg
     1801 ctgaatgcct gctgaggtct ccaggtgttt cccgtcgatc atgtagatcg tgaggcgacc
     1861 accatcggtc gcccgtggtg aaaatgagtt cctggcattg cgctatattt ccgtatggga
     1921 tgcgagacgt ggtagcgggg gatcgaagcg gaaccgacct ccctaccgtc gtcgtggacc
     1981 gattcaccgg ccccctcgcg cgtttcctaa aggtggaggc cgcgaccggc gttctgttgc
     2041 tcctcgccgc ggtcgccgca ctgggcctgg cgaactcacc gtggtccgta tcctttctgg
     2101 cattttggga gacgcccatc ggcctccact tcggcccgtt cgatttcagc cgctcgttgc
     2161 ggcactggat caatgacggg ttgatgacgt tcttcttctt cgtcatcgct ctggagctca
     2221 agcgggcgct gattctcggc gagcttcgtg atctgcgcgt ggcgtcgctt ccgttcgccg
     2281 gagcactcgg aggcatgatc gtaccggcga gcctgtattt cgtgctcatg agcggccggc
     2341 ccggcgcgaa cggctggggc acggtgatgg cgaccgacac cgccttcgtc atcggctgcc
     2401 tggcgctctt cggctctcgc atcccggcga gcctgcgcgt gttcgccgtg tcgttggcgg
     2461 tgttcgatga cgtgggtgcc atcctgatcg tagccgcggt ctacgggggt gccctgaact
     2521 tgaacgctct gaccctcggg atgctcggag tcgtggcggt tgcggtctgt gcccgcctgg
     2581 ggatgaggag tattcccctc tacttcctcc ttggaggcgc ggtgtggctt tgcttcgatg
     2641 cttccggcat ccatgcgacg atggctggcg tcgttctggg tctgatgacg ccgacacgcg
     2701 tatgggtgag cgacgcacgt ctccgaggca tcctcggccg cgccctttcc catccggagg
     2761 gtgagcactg gagcggggac tctgtggagc gaagcgacct gctgcgagcc ggcactgcag
     2821 tcagggagtc gctcagcccg ttggaacggc tggaaatgat gctccaccca tgggtcggat
     2881 tcctgatcat gccattgttc gctctggcca atgccggcat cgcgatctcc ggtgccgaca
     2941 tcggacagcc cgtctccgtg gcgatcttcg ccgcactggt cttcggcaag ccggtcggcg
     3001 tcctgttctt cagtgggatc gccgtacgcc tcggattcgc catccgcccg cttggcttgg
     3061 actggc
//
LOCUS       DBNHOX010000164         1936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_527399, whole genome shotgun sequence.
ACCESSION   DBNHOX010000164 DBNHOX010000000
VERSION     DBNHOX010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_527399"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="AC16UZ_03540"
     CDS             complement(<1..231)
                     /locus_tag="AC16UZ_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR6245786.1"
                     /translation="MTQAYDYIIVGAGSAGCVLAYRLSADPGVNVLLLEAGGVDRSPL
                     IGIPKGIGKLVRSPDHAWFFPVEQPREPDLPAS"
     gene            complement(233..823)
                     /locus_tag="AC16UZ_03545"
     CDS             complement(233..823)
                     /locus_tag="AC16UZ_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR6245787.1"
                     /translation="MSKTVEIFFDFLSPPSYLAWTQVPGIVQRTGASAVWRPMFTIGL
                     HQLTENRSPVMVPNKARWIAGDLQRFARRYGVTLNPNPHGIINILPADRAAALAEKEG
                     AVEQLMATAYPAMWVDGRDLSDPEVLGEVIAAAGLDASRYLAAIETDEVKNLLKQNTQ
                     EAADRGAFGAPTFFVGDEMFFGQDRLDFVEEALAGG"
     gene            complement(845..1576)
                     /locus_tag="AC16UZ_03550"
     CDS             complement(845..1576)
                     /locus_tag="AC16UZ_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003210139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6245788.1"
                     /translation="MTTSEKVVLVIGAGVATGGAVARRFAREGFTACMARRQGEKLQG
                     LVDEIAAAGGRAHGYSCDATEEAQVVDLVERVEAEVGPIEVACYNAAVGASHSITEFP
                     ADAYERVWRINTFGAFVMGREVSRRMVERGRGTILFTGATSALRGKARLAAFAGSKHA
                     LRALSESMARELFPQNIHVAHVIIDGPIDTPLIRRVMPSVFEERPADGVLLPDDIAET
                     YWNIHCQPRSAWLHETQLRPWLEPW"
     gene            complement(1648..>1936)
                     /locus_tag="AC16UZ_03555"
     CDS             complement(1648..>1936)
                     /locus_tag="AC16UZ_03555"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245789.1"
                     /translation="TEQVWEDAVAGRTGDPAHVAALFAAAQHAIAIARDTVDGAFALV
                     GTAALYVDNPLERAHRDMHAMLRHILAQPSWLEQAGRLEFGLEITEPMFFV"
BASE COUNT          334 a          659 c          634 g          309 t
ORIGIN      
        1 ggaggccggc aggtccggct cccgcggctg ctcgaccgga aagaaccagg cgtggtcggg
       61 gctcctgacc agcttcccga tgcccttggg tatgccgatg agggggctcc tgtccacccc
      121 acccgcctcc aggagcagaa cgttcacgcc gggatctgcg gagagccgat aggccagcac
      181 gcagccggcc gaaccagcac cgacgatgat gtagtcgtac gcctgggtca tctcagcccc
      241 ccgccagcgc ttcttcgacg aaatccaggc gatcctggcc gaagaacatc tcgtcgccca
      301 cgaagaaggt cggagcgccg aaggcacccc gatcggcagc ttcctgggtg ttctgcttga
      361 gcaggttctt cacctcgtcg gtctcgatgg cggcaaggta gcgggatgca tcgagaccgg
      421 cggcagcgat gacctcgccg agcacctcgg ggtccgacag gtcgcggccg tccacccaca
      481 tggccgggta cgccgtggcc atgagctgct ccactgcgcc ctctttctcc gccagcgcag
      541 cggcacggtc ggccggcagg atgttgatga tgccatgggg attgggattc aaggtgacgc
      601 cgtaacggcg cgcaaatcgc tgcaggtcgc cggctatcca gcgtgccttg ttcggcacca
      661 tgaccggcga acggttctcc gtaagctggt gcaggccgat ggtaaacatg ggtcgccaga
      721 cggcgctggc gccggtccgc tggacgatgc ccggcacctg ggtccacgcc agatagctcg
      781 gcgggcttaa gaagtcgaag aaaatttcca cggttttgct cacgatgaca ccacctccaa
      841 ccgtctacca gggctccagc caggggcgca gctgggtttc gtgaagccat gcgctgcggg
      901 gctggcagtg aatgttccaa tacgtctcgg cgatgtcgtc gggaagcaga acgccgtccg
      961 caggacgctc ctcgaaaacc gacggcatca cgcggcggat gagcggcgtg tcgatgggcc
     1021 cgtcgatgat cacgtgggcc acgtggatgt tctgcgggaa aagctcccgg gccatgctct
     1081 cgctcaatgc gcgaagcgcg tgcttcgacc ctgcaaaggc cgcgagccgg gctttcccgc
     1141 gcagcgcgga ggtggcgcct gtgaagagga tggtcccacg cccccgctcc accatgcgcc
     1201 gggacacctc ccgccccatg acgaaggcac cgaaggtgtt gatgcgccag acgcgctcgt
     1261 aggcatctgc cgggaactcg gtgatgctgt gggatgcacc cacggcggcg ttgtagcagg
     1321 ccacctcgat gggtccgacc tcggcttcca cccgctcgac cagatccacg acctgggctt
     1381 cttcggtcgc atcgcagctg taaccatggg cccgtccgcc cgccgcggcg atctcgtcca
     1441 ccagcccctg cagcttttct ccctgacgtc gcgccatgca ggcggtgaaa ccctcccggg
     1501 caaaacgccg cgccaccgcg ccgccggtgg cgaccccggc cccgatgacg agcaccacct
     1561 tctccgaagt tgtcataggc aatccccctt ggcccgacag taacccaccc ggattcccga
     1621 atatagcgcg agccgccggt caggagttca gacgaagaac atcggttccg tgatctcgag
     1681 cccgaactcc agccggcccg cctgctccag ccaggacggc tgggcgagga tgtggcggag
     1741 catcgcgtgc atgtcgcgat gggcgcgctc gagggggttg tccacgtaga gggcggcggt
     1801 gccgacgagg gcaaaggcac cgtccacagt atcccgggcg atcgcgatgg cgtgctgggc
     1861 ggcggcgaag agcgcggcga cgtgcgcagg gtcgcccgtc cggccagcca ccgcatcttc
     1921 ccagacctgc tccgta
//
LOCUS       DBNHOX010000165         2194 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_12935, whole genome shotgun sequence.
ACCESSION   DBNHOX010000165 DBNHOX010000000
VERSION     DBNHOX010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_12935"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="AC16UZ_03560"
     CDS             complement(<1..172)
                     /locus_tag="AC16UZ_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6245790.1"
                     /translation="MSYDVIVLGVGAMGAAALERLASRGARVLGIEQWSVPNSLGSSG
                     GDTRLIRKAYFEH"
     gene            291..1364
                     /locus_tag="AC16UZ_03565"
     CDS             291..1364
                     /locus_tag="AC16UZ_03565"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR6245791.1"
                     /translation="MDPVSQAALGAVVARSVSHRQLGAGAVALGAAAGALPDIDVFFS
                     LDGDYFDQLVSHRGITHSLFFAPVVGPLFGWLAWRWTARRRNDPGEPAALRAWMLAMT
                     LAMLSHPLLDLLTPYGTQLLQPFSDARFAVAAMPIIDPIYTIVLLAGLVAGYRFTAAG
                     GRIAAATLLVSCAYLGYGWHLNEGAERFARAQLAAQGLPNVEVAAFPTILQIHHRRVV
                     ARTPDADRVGFLSMWDPCDIGWQREPRLPRSQVEAFLRTREGAIFDWFTMGWSHYAIQ
                     DGHMTATDLRYGSDENPRASVFTASVALDHLGRPSGNIAGGRFTPDANGRNLERLIAM
                     TYAPGCGTSPGRMAQTSLEAGAG"
     gene            1414..>2194
                     /locus_tag="AC16UZ_03570"
     CDS             1414..>2194
                     /locus_tag="AC16UZ_03570"
                     /inference="COORDINATES: protein motif:HMM:NF015515.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HIR6245792.1"
                     /translation="MPSGPMDDDGLTMEQEAGRPNDPSNLDVVVAIVPTTVLLALSVA
                     LFGSDSSSGANQIALVIGAALATMVGVKNGHSWKVIEENIVGGISTAMPAIMILLSVG
                     SLIGVWMLSGTVPTMIYYGLLLLEPAVFYAASCLLCAVTALSIGSSWTVAGTLGIGLI
                     GVAAGLELSLPITAGAIISGAYFGDKLSPLSDTTNLAPAVTEIDIFTHIRHMTWTTLP
                     SFVFALGAFTAIGLMESTPETQTSLRVTLETLDEAFAINPLM"
BASE COUNT          366 a          755 c          668 g          405 t
ORIGIN      
        1 gatgctcgaa gtaagccttt cggatgaggc gcgtatcacc cccactggag cccaaggagt
       61 tgggcaccga ccattgctcg atgcccagca ccctggcgcc ccgggatgcc agccgctcca
      121 aagccgcggc gcccatggcg ccgacaccga ggacgatcac gtcgtagctc atggcgcaat
      181 cctatctcag ctcggcgaag cgggcagagg gaacagtgca cattgcggct gccaatcggc
      241 tccatcgccg gtatgatgct cgcaactgct ccagccgtcg tgtcacttcc gtggatcctg
      301 tctcccaagc cgccctcggc gccgtggtcg cccgcagtgt cagccatcgc caacttggtg
      361 ccggcgccgt cgcgcttggg gccgcggcag gcgccctgcc ggacatcgac gtgttcttct
      421 ccctggatgg tgactacttc gaccagctgg ttagccatcg cgggatcacg cattcgctgt
      481 ttttcgcccc cgtggtgggc cccctcttcg ggtggctggc ctggcgctgg accgcccgcc
      541 gccgaaacga ccccggggag ccggcggcat tgcgcgcctg gatgctcgcg atgaccctgg
      601 cgatgctttc ccacccgctt ctagatttac taacgccgta cggcacgcaa ctcctccagc
      661 ccttcagcga cgcgagattc gcggtggcgg ccatgcccat catcgatccg atctacacca
      721 tcgtgttgct ggctgggctc gttgccggat atcggttcac ggcggccggg gggaggatcg
      781 cggcagcaac gctgctcgtc tcctgcgctt acctgggtta cggctggcac ctcaacgagg
      841 gcgcggagcg gtttgcccgc gcccagctcg cagcccaggg gttgccgaac gtcgaggtag
      901 ccgcgttccc gacgatcctg cagattcacc accgacgtgt ggtcgcacgc accccggacg
      961 ccgaccgggt cgggttcctc tccatgtggg atccgtgcga tatcggctgg caaagggaac
     1021 ctcgcctgcc ccgatcacag gtggaggcct tcctccgcac gcgggaaggg gcgatattcg
     1081 actggttcac catgggttgg tcacactacg ccatccagga cgggcacatg acggcaaccg
     1141 atcttcgcta cgggtctgat gagaaccccc gggcaagcgt tttcacggca tcggtcgccc
     1201 tggaccacct cgggcgcccc tcaggaaata tcgccggcgg ccgcttcacc ccggacgcca
     1261 acggccggaa cctcgaacgg ctgatcgcca tgacctacgc acccggctgc ggcacgagcc
     1321 ctggacggat ggcccaaacg agcctggaag ccggcgccgg ctgatggcgt caacggagca
     1381 tctgcaacga tcgcttcgaa cgcagtcagg gccatgcctt ccggacccat ggatgacgac
     1441 ggtctgacca tggagcagga agccggccgg ccgaacgacc caagcaatct ggacgtcgtg
     1501 gtggccatcg tcccgacgac ggtcctcctg gcgctgtcgg tcgccctctt cggcagtgac
     1561 tcgtcgagtg gcgccaatca gattgccctg gtcatcggag ctgcgctggc caccatggtc
     1621 ggcgtgaaga acggccattc ctggaaggtg atcgaagaga acatcgttgg cggcatttcc
     1681 accgcgatgc cggcaatcat gattctcctg tcggtcggca gcctcatcgg ggtgtggatg
     1741 ctgtcaggaa ccgtgcccac gatgatctat tacgggttgc tgcttctcga accagcggtg
     1801 ttttacgccg cgagttgtct cctgtgcgcc gtgacggcgc tctccatcgg aagctcctgg
     1861 acggtagcgg gcacgctcgg catcggtctg atcggggtag ccgccgggct cgaactttcg
     1921 ctgccgatca ccgctggggc catcatctcc ggagcctatt tcggcgataa gctctcccca
     1981 ctttccgata ccacgaatct cgccccggcc gtgaccgaga tcgacatctt cacccacata
     2041 cggcacatga cctggaccac cctgccgagc ttcgtgtttg cccttggggc gttcaccgcc
     2101 atcggcctga tggaaagcac gcccgagacg caaacgtcac tgcgcgtcac cctggaaacc
     2161 ctcgacgaag cttttgccat caaccccctg atgc
//
LOCUS       DBNHOX010000166         1977 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1363005, whole genome shotgun sequence.
ACCESSION   DBNHOX010000166 DBNHOX010000000
VERSION     DBNHOX010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1363005"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="AC16UZ_03575"
     CDS             <1..507
                     /locus_tag="AC16UZ_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HIR6245793.1"
                     /translation="FDLTPYYGFEEDAVHWHAQAREAAGEHYPAMKAACDEYFYLPHR
                     QECRGIGGVFFDDWTGGGFDASFTLTKAVGDHFLPAYQPIFERRMDNVYGERERDWQL
                     YRRGRYAEFNLAIDRGTKYGLQSGRRVESVLASLPPLAAWKYDYHPEPGTPEARLYAD
                     FLPPRDWV"
     gene            857..1777
                     /locus_tag="AC16UZ_03580"
     CDS             857..1777
                     /locus_tag="AC16UZ_03580"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR6245794.1"
                     /translation="MSELTVLAEGLRFPEGPIAMPDGSVLVVEIARGTLSRVANGDVA
                     VVADLGGGPNGAAIGPDGHCYVCNNGGFQWIEHRGRLFPGEEPENYSTGRIEKVDLET
                     GAIEVLYTHCEGQPLRGPNDLVFDRHGGFWFTDHGKTRARERDRTGVFYAKADGSYIE
                     EVIFPLEGPNGIGLSADENELYIAETPTGRLWAYELDGPGQLLGERRDKPDGGRLVRG
                     RQGYFFFDSMAVDANGNVCVATIADGGITVLKPAGGEPEHVPMPDTLTTNICFGGEDL
                     KTAFITLSSTGKLVSMPWLTAGLPLNYLNK"
     gene            1783..>1977
                     /locus_tag="AC16UZ_03585"
     CDS             1783..>1977
                     /locus_tag="AC16UZ_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6245795.1"
                     /translation="MAYIDRGGVRIWYETAGSGPTLLLTHGFSATSFAWQPQMAALSD
                     AYQVVVWDIRGHGRSDSPDDP"
BASE COUNT          372 a          641 c          599 g          365 t
ORIGIN      
        1 ttcgacctca ccccctatta cggctttgag gaagacgccg ttcactggca cgcccaggcc
       61 agggaggcgg cgggcgagca ctaccccgcc atgaaggccg cctgcgacga gtatttctat
      121 ctgccccatc gccaggagtg ccgaggcatt ggaggcgtgt tcttcgacga ctggaccggg
      181 ggtgggttcg atgcgagctt cacgctgacg aaagcagtgg gcgaccactt tcttcccgcc
      241 tatcagccca tcttcgaacg gcgcatggac aacgtttacg gagagcgcga gcgcgactgg
      301 cagctttacc gccgcggccg ctacgcggag ttcaacctgg ccatcgaccg ggggacgaag
      361 tacggcctgc agagcggccg ccgggtcgag tccgtgctcg cctccctgcc cccgctggcg
      421 gcgtggaaat atgactacca ccccgagccc ggcacccccg aggccaggct ttatgcggac
      481 tttctgccgc ccagagattg ggtctgaggt tgtggataac tctggggatt aattctgaat
      541 gcttttcacg aatctgtcat ttgagggcga tatcggccga tcgggataga gaaatcgtat
      601 ttaaaacaat atgttataaa ttttttctcc aacaaatgtt ccaacgcagg tgcgcatttt
      661 ctggcctcac gattgtgcat aagagtcaag cccctagggc ccataaagtg caactcagtg
      721 ttcccagagg taacagatca ggctgattta ccgcccgcta cgcccaagct catcggtcac
      781 gcaacgcaac gttcttggcg gtgtgcaggc ccgccgctag aatgcctatc ccaatatcga
      841 cgacgaggac cgccccgtga gcgagctcac cgtcctggcc gaaggcctcc gctttcctga
      901 gggtcccatc gccatgcctg acggcagcgt gctggtcgtg gagatcgcgc ggggcaccct
      961 ttccagagtc gccaacggcg acgtagctgt ggttgccgat cttggcggcg ggcccaacgg
     1021 cgccgccatc ggccccgacg gccattgcta cgtctgcaac aacggcggct tccagtggat
     1081 cgagcaccgc gggcggctct ttcccggcga agagccggag aactactcca ccgggcgcat
     1141 cgaaaaggtc gatctggaga cgggcgccat cgaggtgctc tacacccact gcgagggcca
     1201 gccgttgcgg ggtcccaacg atctcgtttt cgatcgccat ggtggtttct ggtttaccga
     1261 ccacggcaag acgcgggctc gggaacggga ccgcaccggc gtcttctatg ccaaggcgga
     1321 cgggtcctac atcgaggagg taatcttccc gctggaaggc cccaacggca tcgggctttc
     1381 tgcggacgaa aacgagctct acattgccga gacacccacc gggcgccttt gggcatacga
     1441 gctcgacggc cctgggcaac tgctcggcga acgacgggac aagcccgacg gcggccgcct
     1501 ggtgagaggc aggcagggct atttcttctt cgactccatg gcggtggacg cgaacggcaa
     1561 cgtgtgcgtg gccaccatcg ccgacggtgg catcaccgtg ctcaagccgg ccggcggcga
     1621 gcccgagcac gtgcccatgc ccgacaccct gacgaccaat atctgctttg ggggcgagga
     1681 tctgaagacg gccttcatca cgctctcctc caccggcaag ctcgtctcca tgccctggct
     1741 caccgccggc ctgccgctga actatctgaa caagtaatca ccatggctta catcgaccgg
     1801 ggcggcgtcc ggatctggta cgagacagca ggctccggtc ccacactgct gctcacccat
     1861 ggcttcagcg ccacatcctt cgcctggcaa ccgcagatgg cggcgctctc cgatgcctat
     1921 caggtggtgg tctgggacat ccgcggccac ggccgctcgg acagtccgga cgatccg
//
LOCUS       DBNHOX010000167         3029 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1298980, whole genome shotgun sequence.
ACCESSION   DBNHOX010000167 DBNHOX010000000
VERSION     DBNHOX010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1298980"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..979)
                     /locus_tag="AC16UZ_03590"
     CDS             complement(41..979)
                     /locus_tag="AC16UZ_03590"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR6245796.1"
                     /translation="MQFKNRVTEMLGVEIPVVQAPMGWIARSQLASAVSNAGAMGIIE
                     TSSGELDAVREEIRRMRELTDKPFGVNIAQAFVRDPNIVQFVIDQGVKFVTTSAGNPE
                     RFTDELKSAGLTVFHVVPSLSAALKAVACGVDGLVVEGGEGGGFKNPREVSTMVLLPL
                     VRSKVDVPIIAAGGFVDGASMAAAFALGAEGVQLGTRMVSALESPVHQNWKDAIVNAG
                     ETDTVFLNRKHSPALRALRTEKTTRLENEPDTNAMSEFGSALDLYFGGDMEASIALSG
                     QVCGRIDAVRPVAEIIAELKDEFFGTIARLDEQYLS"
     gene            1186..2472
                     /locus_tag="AC16UZ_03595"
     CDS             1186..2472
                     /locus_tag="AC16UZ_03595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6245797.1"
                     /translation="MQARPPKPFKARDLTADRATAMRFTAVLGLQMGQIFPGSFIALF
                     LPVIYREQGLSLEMYWVFTLPAIPTWIRPLWAPFVDRTGSRIIGMRKTWFIPCTVFGA
                     LAYLSLTQFAPTLENLAVIITILVIKTTFMTTQDIAIDGYMVENIRDEERPVGAAVLD
                     IGRNVARFIAWAGIAWVYTEHGWSAAVTVASALLILFSAPGIIRREPPRPSQFDHVRP
                     SLKNLLRRRDSYYVYPLCFGIALMGALVQSLYPTFLSDLGFSLGEVAAVIAPAILIGS
                     VLGASVTSWYLGRFGYKATVLTAAFAVVLAMLPIMWMGSLEEPTFVVVFAVTLNAIAL
                     PSFLDVSFQAARLKWASKSQAATDYTSQIVTMAAGGGLAVAVGGFMAEYLGWFYYFLV
                     AGTLVSGACFVLYYLFDTIEDIVEERDRTEAEGGNL"
     gene            2532..>3029
                     /locus_tag="AC16UZ_03600"
     CDS             2532..>3029
                     /locus_tag="AC16UZ_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6245798.1"
                     /translation="MALVDYEIHGHIAVITLNRPEARNAINPEVAVRLADAWESVRDD
                     DQVRVAILTGTGSAFCAGADLGQLIPMVSGTRKPENEWDERVASEPGITNRALLRNFD
                     TEKPIIAAINGHAIAGGMEIVQGTDIRVSVAEAKFGVQEVKWAIFPAGGSTVRLPVQL
                     PYARAL"
BASE COUNT          530 a          992 c          957 g          550 t
ORIGIN      
        1 gcccgagcgg catcgcgttg gagttcgagc ggccctgaac tcagctcaga tactgctcgt
       61 ccaggcgcgc gatggtcccg aagaactcgt ctttcagctc ggcgatgatc tccgccaccg
      121 ggcgaacggc gtcgatgcgg ccgcaaacct gacccgacag agcgatgctc gcttccatgt
      181 cgccaccgaa atagagatcc agcgcggagc cgaactcgct catggcgttg gtgtccggtt
      241 cgttctccag ccgcgtcgtc ttctccgtgc gcagcgctct cagcgcgggc gagtgcttgc
      301 ggttgagaaa caccgtgtcc gtttcgccgg cattcacgat ggcgtctttc cagttctgat
      361 gcacgggtga ctccagcgcg gacaccatcc gcgtgccgag ctgaacgccc tccgcgccga
      421 gcgcgaacgc tgccgccatg gacgcgccgt ccacgaaccc gcccgcggcg atgatgggaa
      481 catccacctt cgagcgaacc aggggcagca gaaccatggt ggagacctcg cgtgggttct
      541 tgaagccgcc gccctcgccg ccttccacca ccagtccatc cacgccgcag gccacggcct
      601 tcaacgcagc gctgaggctg ggaaccacgt gaaagacggt gagcccggcg ctcttgagct
      661 catccgtgaa ccgctccgga ttgccggcag acgtcgtcac gaacttgacg ccctggtcga
      721 tgacgaactg aacgatgttg gggtcgcgta cgaacgcctg ggcgatgttg acaccgaacg
      781 gcttgtcggt gagctcgcgc atcctgcgga tttcctcccg aacggcgtcg agctccccgg
      841 aggacgtttc aatgatgccc atggcgcccg cgttcgacac ggcggaggcg agctgcgagc
      901 gggcaatcca tcccatcgga gcctgcacga cgggtatctc gacccccagc atctcggtta
      961 ctcggttctt gaactgcatg acgccccccg gggaaatcgg ggagcctacc tcagctgccg
     1021 gtggacgtgt agtggcgcag gccgaatcgt cagaggaaga cggccagccc gaaatagaag
     1081 aacccagcag ggtgcgggtg gccggaaagg gccgcggatt ctactggagc gtcgatcgca
     1141 gaaacaaccc gtgtgccatc cgggcgccgc cgctaaagtg gccggatgca ggcccgccca
     1201 cccaagccgt tcaaagcgcg cgacctgacc gccgatcggg cgacggccat gcgcttcacc
     1261 gcggtgctcg gccttcagat gggtcagatc tttccgggct cgttcatcgc cctgtttctg
     1321 ccggtgatct accgcgagca ggggctgtcg ctggagatgt actgggtctt cacgctcccc
     1381 gccatcccga cctggatacg gccgctctgg gcgccgttcg tcgaccggac gggcagccgc
     1441 atcatcggca tgcgaaaaac ctggttcatt ccctgcacgg tcttcggcgc tcttgcgtat
     1501 ctcagcctga cccaattcgc gccaaccctc gaaaatctcg ccgtgatcat cacgattctg
     1561 gtgatcaaga ccacgttcat gaccacccag gacatcgcca tagacggcta catggtcgag
     1621 aacatccggg acgaggagcg gccggtgggc gccgccgtgc tggacatcgg ccgcaacgtg
     1681 gccaggttca tcgcgtgggc cggcatcgcc tgggtctaca cggagcatgg ctggtcggcc
     1741 gcggtcaccg tggcctcggc tctgctgatc ctgttctccg caccaggaat cattcgcagg
     1801 gaaccgccga ggccgtccca gttcgaccat gtccggccga gcctcaagaa cctgcttcgc
     1861 cgtcgcgaca gctactacgt ctaccctctc tgctttggaa tcgctctgat gggcgcgctg
     1921 gtgcagtcgc tgtacccaac gtttctttcc gatctgggct tcagcctcgg cgaggttgcc
     1981 gccgtgatcg ccccggccat cctgatcggt tcggttctgg gggcgtcggt caccagctgg
     2041 tatctgggac gattcgggta caaggcaacg gttctcacgg cggcgttcgc ggtggttctg
     2101 gcgatgctgc ccatcatgtg gatgggatcg ctggaggaac cgacattcgt cgtggtcttc
     2161 gcggtgaccc tcaacgccat tgctctgcct tcgttcctcg atgtgagctt tcaggctgcg
     2221 cgcctgaagt gggcgtccaa atctcaagcg gccaccgact acacctcgca gatcgtcacc
     2281 atggcggcgg gcggggggct ggcggtcgcc gtgggcggtt tcatggccga gtacctggga
     2341 tggttctact acttcctggt cgcgggaacc ctggtgagcg gcgcctgctt cgtgctctac
     2401 tatctcttcg acacgatcga agacatcgtc gaggagcgcg accggacgga agccgagggc
     2461 ggtaacctct gacccttcac gagcagagcc aagcccgcgt cacaacaaat gcagaggaag
     2521 gggaggatcc aatggcactc gtagactacg aaattcacgg acacatcgcg gttatcactt
     2581 tgaaccggcc ggaagcccgc aacgcgatca atccggaggt ggccgtacgc ctggcagatg
     2641 cctgggaatc ggtacgtgac gacgaccagg tccgcgtcgc catcctgacg ggcacgggca
     2701 gcgcgttctg cgccggggcc gatctgggcc agctcatccc aatggtctcc ggaacgcgca
     2761 agcccgagaa cgagtgggac gagcgtgtgg cttccgagcc gggcatcacc aaccgggcgc
     2821 tgctgcgcaa cttcgatacc gagaagccga tcattgcggc aatcaacggc cacgccatcg
     2881 caggtggcat ggagatcgtc cagggcacgg acatccgcgt ctccgtggcg gaggcgaagt
     2941 tcggcgttca ggaggtcaag tgggccatat tccccgccgg cggatccacg gtgcggctgc
     3001 cggttcagct gccctacgcg cgcgctctg
//
LOCUS       DBNHOX010000168         1879 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1106380, whole genome shotgun sequence.
ACCESSION   DBNHOX010000168 DBNHOX010000000
VERSION     DBNHOX010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1106380"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="AC16UZ_03605"
     CDS             <1..1139
                     /locus_tag="AC16UZ_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245799.1"
                     /translation="DPALRLSRAHPRQINQHYLARMNADPLPYPVSNAANHADLVYLH
                     LKQLQDLMRRDPGDLVLAVPAILSADQLGVLLGITQECGMAVSGFVDSAVAAASVSEL
                     PAIVCHLDAHLHRTCLTLLDVGADVVRTGADEVADCGVANLLEGWVNVIADRFVRDTR
                     FDPLHAAETEQQLYNQVYDWAMGQSSSDSEIGIEIGYRDQVRRVEVSRSLLEEKARQR
                     FAHLSERLPEDVHLALSARTARLPGLTAYLGAQGWEYSVLDGSALAAGCSANDSSIRA
                     EDGRLRLVSRLPLRHGRGPQSIVAPGPDESGRPTHVLCDACAVPIGADDLPLQLKESE
                     AGVYLDADETVRLNGEAITTSRRLRAGDELTAGGKSYLLIRVVG"
     gene            1141..>1879
                     /locus_tag="AC16UZ_03610"
     CDS             1141..>1879
                     /locus_tag="AC16UZ_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245800.1"
                     /translation="MARNRRFSVFSLSFLDVMSCGFGAVVLIFLIINHAAEEDAKVVN
                     EELLSEIRLLDYQVQNGERDLFELKEQLDAILQRLSDSSRKLVSTETSLKETSKEFAD
                     LAAESLAQEASLEELKSDVDTREEELARLKSINEANEGGRIRSFTGEGDRQYLTGLKV
                     GGKHILIAVDTSTSMLDESIVNVIRRRNMPDDRKRMSPKWQRAVRTVEWLTAQLPLDA
                     SFQIYGFNVEARSLLEEHHGQWIALAEG"
BASE COUNT          348 a          614 c          609 g          308 t
ORIGIN      
        1 gcgaccccgc gcttcggctg tcccgcgcac acccccgcca gatcaaccag cactacctcg
       61 cccgcatgaa cgcggacccg ctcccctacc ccgtttccaa cgccgctaat cacgcggatc
      121 tggtctacct gcacctcaag cagcttcagg acctgatgcg gcgcgacccg ggagatctcg
      181 tgctggcggt acccgcgatc ctgagcgcgg atcagctcgg cgtgctgctg ggcataaccc
      241 aggagtgcgg catggcggtg agcggcttcg ttgactccgc agtagcagcg gcgtcggtca
      301 gcgagctgcc ggccatcgtc tgccatctcg acgcgcacct gcacaggacg tgtcttaccc
      361 tgctggatgt cggcgccgac gtcgttcgaa cgggggcgga tgaggttgcc gactgcggcg
      421 tggccaacct gctggaaggc tgggtcaacg tgattgccga ccgcttcgtt cgggacacga
      481 ggttcgaccc tctgcacgcc gccgagacgg agcagcaact ctacaatcag gtctacgact
      541 gggccatggg ccagtcgagc agcgacagcg agatcggcat cgagatcggc taccgggatc
      601 aggtcaggcg ggtagaggtc tcccgcagtc tgctcgagga gaaagcccgg cagcgattcg
      661 cccacctcag cgaacgcctc cccgaggacg tgcacctggc gttgagcgcg cgtacggcaa
      721 ggctccccgg gctgaccgcc tatctgggcg cccagggttg ggaatactcg gtactcgacg
      781 gctccgccct ggcggcgggc tgcagtgcga acgactccag catccgggcc gaggacggcc
      841 ggctcaggct ggtttcccgg ctgccattac ggcacggccg gggtccgcag agcatcgttg
      901 ccccggggcc cgacgagtcc ggccgcccta cccacgttct ctgcgatgcc tgcgccgtgc
      961 ccatcggtgc cgacgatctg ccgctgcagc tcaaggagag cgaggccggc gtgtatctcg
     1021 atgccgacga gacggtccgt ctcaacggcg aggcgatcac caccagcaga cgcctgcgcg
     1081 cgggggatga gctcaccgcc ggcggcaaaa gctatctcct gatccgcgtg gtggggtgag
     1141 gtggcccgca accggcgctt cagcgtcttc tctctgtcgt ttttagacgt gatgtcgtgc
     1201 gggttcgggg ccgtggtact gatcttcctg atcatcaatc acgcagccga ggaggacgcc
     1261 aaggtcgtca acgaggaact cctttcagag attcgcctgc tcgactatca ggtgcagaac
     1321 ggtgagcggg accttttcga gctcaaagag cagctcgacg ccattctgca gcggctgtcc
     1381 gactcgagcc gcaagctcgt cagcacggaa accagcctga aggaaaccag caaagagttc
     1441 gccgacctcg ccgcggagtc ccttgcccag gaagctagcc tggaagagct gaagagcgac
     1501 gtggacacgc gcgaggagga gctggccagg ctcaaatcca tcaacgaggc aaacgaggga
     1561 ggcaggatac gcagcttcac cggcgagggg gatcggcaat acctgactgg cctgaaggtc
     1621 ggcggcaagc acatcctcat tgccgtggac acgtcgacca gcatgctcga tgagagcatc
     1681 gtcaacgtga ttcggcgccg caacatgccc gacgatcgca aacggatgtc gcccaagtgg
     1741 caacgggccg ttcgcaccgt cgagtggctg accgcccagc tgccccttga cgccagtttc
     1801 cagatctacg gattcaacgt ggaggcgcga tcgctgctgg aagagcacca tggacagtgg
     1861 attgctcttg ccgagggcc
//
LOCUS       DBNHOX010000169         8673 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1620229, whole genome shotgun sequence.
ACCESSION   DBNHOX010000169 DBNHOX010000000
VERSION     DBNHOX010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8673)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1620229"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1432)
                     /gene="ggt"
                     /locus_tag="AC16UZ_03615"
     CDS             complement(<1..1432)
                     /gene="ggt"
                     /locus_tag="AC16UZ_03615"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR6245801.1"
                     /translation="MRNIPATSIKLVLCALVLAACTPSAGVIDKGEPATPQAAVAMPA
                     THARAVAAAVLRNGGNAVDAAVAAAFTLAVVQPEAGNIGGGGFMTIFMDGEARFLDYR
                     ETAPAAASRNMFLGPDGKPDADESLVGHRASGVPGTVAGLWAAHQRYGTRTWAELVAP
                     AVDLARNGFTVYPALAQYAREALTDLAGTNFAAHFAALTTTRRFRQPDLADTLQRIAE
                     NGADEFYRGRTARLLAAEMARGGGLITRADLAGYRAIWRDPIDFRWRDYRVLTAPPPS
                     SGGVAIAQLLKMYEARGGDFADLGHNSAAYVHLNAELAKRVFADRAVYLGDPDFVSVP
                     VDSLIDDDYLRARASEANPAQITRPRPVPVIAEHNDTTHFSIVDGAGNAIANTYTINT
                     SFGSGVVVTGAGFLMNNEMDDFAVAPNSPNYFGLVGSEANAIAPGKRMLSSMSPTIAL
                     RDGKVALVVGAMGGSAIITSVYQVMLN"
     gene            1513..3807
                     /locus_tag="AC16UZ_03620"
     CDS             1513..3807
                     /locus_tag="AC16UZ_03620"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6245802.1"
                     /translation="MTIDHVRGRLLVLPLLLVLLALWGCSRTEEPAPAEPPEPSGAVS
                     AAEIERNSARNAYFGDLHVHTRLSFDAFIFGTRSTPDSAYAYALGEPIDHPAGFQMQL
                     DRPLDFLGVTDHAMYMGMLEEMSNPETRAGQHPIAVGIREAETPASRLEAFQAMFPYL
                     RKQLDGPDDLLDSAVLRSAWQETVAAAERRNDPGRFTSFVAYEYTAGGAERQNLHRNV
                     VFRGAAPELPFSSMDSGNPEDLWAWMDAQRGQGVEALAIPHNSNGSDGLMFELTRVDG
                     SPMDAAYAEQRMRNEPLVEITQVKGTSDAHPLLSPNDEWADFELMEVKVATVEPSRAA
                     GSYVREAYLNGLAMQAEQGFNPYRFGLIGSSDTHVGAGSFDEANYWSKVGIVDATSVQ
                     RGSVPMDEPDADGNLYVGGDVATFQDWGASGLAGVWAEANTREDIYDAFRRKETFATT
                     GPRMQVRFFAGFGLEAAEQAGPDRISALYRDGVPMGGDLVGGDADTAPHFFLWALSDP
                     ASARLQRLQVIKGWIDAEGRSQEKVFDVACSDGLEVDEATHRCPDNGARVNLADCSIS
                     QNVGAAELESLWKDPEFDAGQMAFYYVRALENPTCRWSTWDAVRAGVEPRADLQAAIQ
                     ERVWSSPIWYVPGGLAGDQPEAEPAAASLVGVRWLWVGSTIGSADVEVAEPHRYNLLL
                     HSDGGAEFRLDCNQGRGGYEMEGEQLRFGPIMSTRMACPEDSKDFEFGQQLDGVTAFS
                     MDGGALELRLKSEGDVMRFRAETD"
     gene            complement(3864..4508)
                     /locus_tag="AC16UZ_03625"
     CDS             complement(3864..4508)
                     /locus_tag="AC16UZ_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024978.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HIR6245803.1"
                     /translation="MSAANLARSAFAAAVVAMISCSSPGIESFHDYNPNVNFARYQTW
                     SFISTNPMIVSTAAGAVNPLLEGRIMRAVRTDLAGKGFRYADNPENADFVVSFTVGSR
                     EQIKVDQYPASYQVGYSRYHRYGYGGYGYGMSYGTETRVRQYTEGQLAVDVFDVATRS
                     PAFHGSATKRISDSDRENPEALIAAVVGEALTGFPPGSAAGVAQPQLIPYEDQN"
     gene            4658..5485
                     /locus_tag="AC16UZ_03630"
     CDS             4658..5485
                     /locus_tag="AC16UZ_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245804.1"
                     /translation="MSLRKFWVVVSGLLLGSVALAGESPKFGDTVSLRLGGMIHKADA
                     TFSDTRIDRPPVELDLKGLGMDKRYDSFWAGLEWQFAKRWGLTLSYSDFSTDGERSVA
                     EGGNFGDLEWSVGAELTSEMDMKIYIVDVTWDFVKTERSHFGLGLGAHVADFSMEIDL
                     SVEGQIGDDTAIIAGGTETAGITAPLPNISMRGGHRFGDNFYLGGTAGYFSLKVGDID
                     GELITARGSLEWRPHKRFGLGIGYQFVSFDVQEKKETKQERYDADFYGPIFFVSAGF"
     gene            complement(5535..6509)
                     /locus_tag="AC16UZ_03635"
     CDS             complement(5535..6509)
                     /locus_tag="AC16UZ_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR6245805.1"
                     /translation="MSANCSDWLKAGLLCAALAVLGSCVTRAEQVASQSDMSWFARTV
                     SNSPLADADPDDFYEDASKMVRTDIEFHGVVLSEEGNPLKGARVRTSVFDRLADPFEF
                     PFFAFAAREPVFTGDDGRFSVRERGAGMYVTVEVPGWAPVTAPRKLYVYAEGLNQTLP
                     FPTREAPEEFRFERRPPEEELRPIQTGALRLPEDGVPLEVSLRQVAPYGVEPGTGEAI
                     ITCERTADDAAPDDLFDWWCELTVPGGGFQPFRIDMDQAPEEGYVESSRLEYRAADPR
                     WDDRAERSLIIRFADGMYGFIEVSMRMDGDFYVAFSGHWNPTGSTWLD"
     gene            complement(6490..8181)
                     /locus_tag="AC16UZ_03640"
     CDS             complement(6490..8181)
                     /locus_tag="AC16UZ_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245806.1"
                     /translation="MRRFLPWLLPTALLAGHAAAADVRIGVDWNGDGLIQTLPGSRVP
                     QDAPSDSQPFVFWVNHDQDDLETGGETWPIARPDANTPAPDSSRDLEDFTRLHVEIDD
                     LGSLPGDAVLTLAWASGSTAELNLFRNEDPDCSRRYLLDAEAGRRQLSAPHGTPIGTV
                     GANALKIRLQTLGEPGLDGAGYCFLLEVASPGQGHLVATIADGEGVLARSEPIAAELR
                     HIKSLYQRTTMTWPEEILAPWAYTTEAPPVPDLQWSKDPQGHEFEPGWWETEDHVVWV
                     YGWLKSGEGLREMATTQAAETIFKRLWHRGYRGRLTFFHWPTVKPRYAYGLLESEYRA
                     YKSAPALVDFVSQIPKGKRIHVTAHSLGCVLVTEAIKLGLEAEEALLQVGAIPAAAYD
                     TRPVLTLPDMADVVTPWLPEEGGYHGYIDETPTPVYTMYNYADVTFFGWNIAQKQMKP
                     TAKRGGYRYAWEPDAPQGERARLYYKTGWFSSKYRLVDDPHEIMAFIAKAKTHAIGAE
                     TRVQGHVRRVFDLARKPHDFHTGHVVGWTRPVHETTAFYNLLLDVWNIAYVSELL"
     gene            complement(8234..>8673)
                     /locus_tag="AC16UZ_03645"
     CDS             complement(8234..>8673)
                     /locus_tag="AC16UZ_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR6245807.1"
                     /translation="GHEIGHHMTEDEVTVSLKPAAMAEKFHRAAQTLDAFAPITWFRP
                     GSGRYNDAVLELTRARGYRIALASVAPLDTVIHNSARMAGFVERMIEPGSVVVLHDVG
                     ERGRRTAETLKLLLPKLKERGYAVVSLSRLDALAGMDTSATEE"
BASE COUNT         1442 a         2711 c         2967 g         1553 t
ORIGIN      
        1 ggttgagcat cacctgatag accgaggtga tgatggccga gccgcccatg gcgccgacga
       61 ccagcgcgac cttgccgtcg cgcagcgcga tggtgggcga catcgaggaa agcatgcgct
      121 tgccgggcgc gatggcgttg gcctcgctgc ccaccaggcc gaagtagttg ggcgagttcg
      181 gcgccacggc gaagtcgtcc atctcgttgt tcatcagaaa cccggccccc gtgacgacca
      241 cgccgctgcc gaaagacgtg ttgatggtgt acgtgttggc gatggcgttg ccggccccgt
      301 cgacgatgga aaagtgggtg gtgtcgttgt gctcggcgat gacgggaacc ggacgcggac
      361 gagtgatctg cgcggggttg gcttcgcttg cccgggcgcg aaggtagtca tcgtcgatca
      421 gcgaatcgac cggcacggag acgaagtcgg ggtcccccag ataaacggcc cggtcggcga
      481 agacgcgctt cgccagttcc gcattgagat gcacataggc ggcggagttg tgcccgagat
      541 ccgcgaaatc gccgccgcgg gcctcgtaca tcttcagcag ctgggcgatg gccacgccgc
      601 ccgaactcgg gggcggagcc gtgagcaccc ggtagtcgcg ccagcggaag tcgatgggat
      661 cgcgccatat ggcgcggtag cccgccaggt ccgctctggt gatcagcccg ccgccgcgtg
      721 ccatctccgc ggccaagagt ctggccgtgc ggccgcggta gaactcgtct gccccgttct
      781 ctgcgatgcg ttggagggtg tcggccaggt ccggctgccg aaaacgcctg gtggtcgtca
      841 gcgcggcgaa gtgggcagcg aagttcgtgc cggcgaggtc cgtaagtgcc tcccgagcgt
      901 attgggcgag ggcgggatag accgtaaagc cgttccgagc caggtccacg gcgggtgcga
      961 ccagctcagc ccacgttcgg gtcccgtagc gctggtgagc ggcccacagg ccggccacgg
     1021 ttcctggcac gccggaggcc cgatggccca ccaggctctc atcggcatcc ggctttccgt
     1081 ccgggcccag gaacatgttg cggctcgccg cagcgggggc ggtctcccgg tagtccagga
     1141 acctggcttc cccatccatg aagatcgtca tgaacccgcc gccgccgatg ttgcctgcct
     1201 cgggttgcac cacggcgagg gtgaaggcgg ccgcgacggc agcatccacg gcgttgcctc
     1261 cgttccggag cacggcggcg gcgaccgccc gggcgtgagt ggcgggcatg gcaacggccg
     1321 cctggggcgt cgcgggctcg cctttgtcaa tgacgcccgc agagggtgtg caagccgcga
     1381 gcacgagagc gcacaggaca agcttgatac tggtggctgg gatgttgcgc atgtcccccg
     1441 gcgctcgctg ccgacagtgg tctaagagcc tgctacatta tctgaaacgt ggagatcttc
     1501 ggaggggtga ggatgacgat cgatcatgtg aggggaaggc tgctggtttt gcccctactg
     1561 ctcgtcctgc tggcgctgtg ggggtgctct cgaaccgaag aacccgctcc tgccgagccg
     1621 cccgagccga gcggtgctgt gtcggcggcc gagatcgagc gaaactccgc ccgcaacgcc
     1681 tacttcggcg acctgcacgt gcacacgagg ctgtctttcg acgctttcat cttcggcacg
     1741 cgctccacgc cggactcggc ttacgcctat gctctgggcg agcccatcga tcatcccgcc
     1801 ggtttccaga tgcagctgga ccggcccctg gattttctgg gagtgacgga tcacgccatg
     1861 tacatgggca tgttggagga gatgagcaac cccgaaaccc gggccggcca gcacccgata
     1921 gcggttggca tccgcgaggc ggagactcct gcatcgcgtc tagaggcgtt ccaggccatg
     1981 tttccttatc tgcgcaagca gctcgatggt ccggacgatc tgctcgattc cgcggtgctt
     2041 cgctctgcgt ggcaggaaac cgtggctgct gccgagcgcc gcaacgatcc gggccggttt
     2101 accagcttcg tggcgtacga atacaccgca gggggagccg agcggcagaa ccttcaccgc
     2161 aacgtggttt ttcggggcgc cgcccccgag ctgccgttct ccagcatgga ctccggcaac
     2221 ccggaggacc tgtgggcgtg gatggacgcc cagcgcggcc agggcgtgga agcgctggcg
     2281 attcctcaca actccaacgg ctccgacggg ctgatgttcg agctgacccg tgtggacggc
     2341 tcgcccatgg acgcggctta cgccgagcag cggatgcgca acgagcccct ggtggagatc
     2401 acgcaggtca agggcacgtc tgacgcccat cccctgcttt cgcccaacga cgagtgggcc
     2461 gacttcgagc tgatggaggt caaggtggcg accgtggagc ccagcagggc ggccggcagc
     2521 tatgttcgag aggcttacct caacggcctg gccatgcagg cagagcaggg attcaacccg
     2581 taccggttcg gcctcatcgg ctcgtcggac acccacgttg gcgcgggctc cttcgacgag
     2641 gccaactatt ggagcaaggt cggcatcgtg gatgcgacct ccgtccagcg cggctccgtg
     2701 cccatggacg agcccgacgc cgacggcaac ctctacgtgg gcggagatgt tgcaacgttt
     2761 caggattggg gcgcgtccgg gctcgccggg gtatgggcgg aggcgaacac gcgtgaagac
     2821 atatacgacg cgttccgtcg taaggagacc ttcgccacca ccggcccgcg catgcaggtc
     2881 cggttcttcg cgggcttcgg gctggaggca gcggagcagg cagggcccga ccggatcagc
     2941 gcgctttatc gcgacggcgt gcccatgggc ggcgatctag tcggcggcga cgccgatacc
     3001 gcgccgcact ttttcctctg ggctctgagt gacccggcgt cggcgcgctt gcagcgtctt
     3061 caggtcatca agggatggat cgacgcagag ggaagaagcc aggaaaaggt gttcgacgtg
     3121 gcgtgctcgg acggcctcga ggtcgacgag gcgacccaca gatgcccgga caacggcgcc
     3181 cgcgtgaatc tcgccgactg ctccatcagc cagaacgtgg gcgccgccga gctggagagt
     3241 ctctggaagg atcccgagtt cgatgccggc cagatggcct tctactacgt gcgcgcgctg
     3301 gagaacccca cctgtcgctg gtctacctgg gacgcggtcc gggccggggt ggaacccaga
     3361 gcggacttgc aggctgcgat tcaggagcgc gtctggtcat cgccgatctg gtacgtcccc
     3421 ggcggcttgg caggcgatca accggaagcc gagccggcgg cggcaagcct cgtcggggtg
     3481 cgttggctct gggtggggtc gacgatcggc tcggcggatg tcgaggtggc ggaaccccat
     3541 cgctataacc tgctgctgca ctcagatggt ggcgcggagt tccgtctcga ctgcaatcag
     3601 ggtcgtggcg gctacgagat ggaaggcgag cagctccggt tcggcccgat catgtcgaca
     3661 aggatggcct gccccgagga ctccaaggac ttcgagttcg gccagcagct cgacggggtc
     3721 actgcgttct cgatggatgg cggcgccctg gagctgcgcc tgaaatccga aggcgacgtg
     3781 atgcgctttc gcgccgaaac cgactgatcg agcgcgcctg agctccgatc accggagccc
     3841 gcgcgtcagt cgagtttgtc agatcagttc tgatcctcat acggtatcaa ctgaggctgc
     3901 gccacgccgg cggctgaccc gggcgggaag cccgtgagcg cctcgcccac gacggcggcg
     3961 atcagcgctt ccggattttc ccggtcgctg tcggatatcc gcttggtggc cgagccgtga
     4021 aaggccgggc tgcgtgtggc tacgtcgaag acgtccacgg cgagttgtcc ttccgtgtac
     4081 tgccggacgc gggtttccgt gccgtagctc atgccgtacc cgtagccgcc gtaaccgtag
     4141 cgatgatagc ggctgtagcc gacctggtag ctggccggat actggtccac cttgatctgc
     4201 tcccgcgatc cgacggtgaa tgaaacgacg aagtccgcgt tctccggatt gtcggcatag
     4261 cggaatcctt tccctgccag gtcggtccgc accgcgcgca tgatccggcc ttccagcaac
     4321 ggattgacgg cgcccgcggc ggtggagacg atcatgggat tggtggaaat gaacgaccac
     4381 gtctgatacc gcgcaaagtt gacgttcggg ttgtagtcgt ggaatgactc gatccccggg
     4441 ctgctgcagg aaatcattgc aacaacggcg gctgcgaaag cggatcgggc aaggttggcg
     4501 gcagacatgc gtgctccctt tgtgtgtgct ttgtgacgcg ggggcgatca tagcggcaaa
     4561 cggaggtcgg cacacgtccg cgatccgcag ctgcacggct atgtataatg ccggctgcag
     4621 aagtgctggg acgttgcaaa gcacgtagga gatgtcgatg agcttgcgga aattctgggt
     4681 ggtcgtgtct ggccttctgt tgggttctgt cgccctggcg ggtgagagcc ccaagttcgg
     4741 agacacggtc tcgctgcggc tgggcggcat gatccacaag gccgatgcga ccttctcgga
     4801 tacccggata gaccgcccgc ccgtggagct cgacctgaag gggctgggca tggacaaacg
     4861 ctacgattcg ttctgggccg gcctcgaatg gcagtttgcc aagcggtggg gcctgacgct
     4921 gagctactcc gatttcagca ccgacgggga acgcagcgtg gccgagggtg gcaacttcgg
     4981 cgatctggag tggtcggtgg gtgccgagtt gacgtccgag atggacatga agatctacat
     5041 cgtcgacgtc acctgggatt tcgtgaagac cgaacgcagc catttcggtc tcggtctggg
     5101 tgcgcacgtt gccgatttca gcatggaaat cgatctgagc gtcgagggtc agattggcga
     5161 cgatacagcc atcattgctg gcgggaccga aaccgcgggc ataacggcgc ccctgcccaa
     5221 tatctccatg cgaggcggcc accgctttgg tgacaacttc tacctggggg gtaccgccgg
     5281 atacttctcc ctcaaggtcg gggacatcga cggcgagctg atcacggcac gggggtctct
     5341 tgaatggcgg ccgcacaagc ggttcggtct cgggatcggc taccagttcg tcagcttcga
     5401 cgtgcaggaa aagaaagaga ccaagcaaga gagatacgac gccgacttct acggccccat
     5461 cttcttcgtc tcggccgggt tctaagagtc gcctctcagg cagctacgga cgccgcgcgg
     5521 cggcagcgaa acgtctaatc cagccaggtg gagccggtcg ggttccagtg cccgctgaac
     5581 gctacgtaga aatctccgtc catgcgcatg gatacttcga tgaaaccgta catcccgtct
     5641 gcgaaacgga tgatgagaga gcgttctgcg cggtcgtccc agcggggatc ggcggcgcgg
     5701 tactcgagac gactggattc cacataaccc tcctcgggcg cctgatccat gtcgatccgg
     5761 aaaggctgaa atccgccgcc cggcacggtc agctcgcacc accagtcgaa gagatcgtca
     5821 ggggcggcgt cgtccgcggt tcgctcgcag gtgatgatgg cttcaccggt gcccggctcc
     5881 acgccgtagg gtgctacctg gcgcagcgaa acctccaggg gcacgccgtc ttcgggtagc
     5941 cgcaacgcgc cggtctggat gggcctgagc tcttcctccg gaggccgccg ctcgaaccgg
     6001 aactcctccg gcgcctcccg ggtgggaaac ggcaacgtct ggttcaatcc ttccgcgtag
     6061 acgtagagct tgcggggcgc ggtcacgggc gcccagccgg ggacctcgac ggtcacgtac
     6121 attcccgccc cccgttcgcg aacgctgaaa cggccgtcgt cgcccgtgaa gactggctct
     6181 cgggcggcga acgcgaagaa gggaaactcg aacgggtcgg ccaggcggtc gaagacggaa
     6241 gtccgcacgc gcgctccctt gagcgggttg ccctcctcgc tgagcaccac gccatgaaac
     6301 tcgatatccg ttcggaccat cttggacgcg tcctcgtaga aatcgtccgg gtctgcgtcg
     6361 gcaagggggc tgttgctcac cgttcttgcg aaccaggaca tgtcgctctg ggacgccacc
     6421 tgctcggccc gggtcacgca gctgcccagc accgcgaggg ctgcgcagag gagtccggct
     6481 ttcagccaat cagagcagtt cgctgacata ggcgatgttc cagacgtcga ggaggaggtt
     6541 gtagaacgcc gtggtctcgt ggaccggtcg cgtccagccc accacgtggc ctgtatggaa
     6601 gtcgtggggc tttcgggcca ggtcgaacac ccgtcggacg tgcccctgaa cgcgggtttc
     6661 cgcaccgatc gcgtgggttt tggccttggc gatgaaggcc atgatctcgt gggggtcgtc
     6721 gaccaaccga tatttggagc tgaaccaccc cgtcttgtag tagagccttg cgcgctcgcc
     6781 ctgcggtgcg tcgggttccc aggcgtagcg atacccgccc cgcttggccg tcggcttcat
     6841 ctgcttctgc gcgatgttcc agccgaagaa ggtgacgtcc gcgtagttgt acatcgtgta
     6901 caccggggtc ggggtctcgt cgatgtaacc gtggtagccg ccctcctccg gcagccacgg
     6961 cgtcaccacg tccgccatgt cgggcagggt gagaaccggg cgggtgtcgt aggcggcggc
     7021 cggtatggcg ccgacctgca ggagcgcctc ctcggcttcc agacccagct tgattgcttc
     7081 ggtgacgagc acgcagccga ggctgtgggc ggtgacgtgg atgcgtttgc ctttcgggat
     7141 ctggctgaca aagtcgacca gggcgggggc ggacttgtag gcgcggtatt cgctctcgag
     7201 gagcccgtag gcgtagcgcg gcttcaccgt gggccagtga aagaacgtaa gccgcccccg
     7261 gtagccgcgg tgccagagcc gcttgaaaat cgtctcggcg gcctgcgtgg tggccatctc
     7321 ccgcaatccc tcgccgctct tgagccagcc gtacacccag accacgtgat cttccgtctc
     7381 ccaccagcca ggctcgaact cgtggccctg ggggtccttg ctccattgga gatcgggtac
     7441 cggaggcgcc tcagtggtgt acgcccatgg cgccaggatc tcttccggcc aggtcatggt
     7501 ggtccgctgg tacagggact tgatgtggcg aagctccgcg gcgatgggct cggaacgggc
     7561 caggacgcct tctccatcgg cgatggtggc gaccaggtgc ccctggccgg gcgacgcgac
     7621 ctccagcaga aagcagtatc cggcgccgtc caaacccggc tcgccgaggg tttgcagccg
     7681 gattttcaga gcgttggccc ccacggtccc gatgggcgtg ccgtggggtg cgctcagctg
     7741 ccggcggccg gcctcggcgt cgagcaggta gcgtcgtgag cagtcggggt cttcgttgcg
     7801 gaagaggttc aattcggcgg tggagccgct cgcccaggcg agtgtcagaa cggcatcgcc
     7861 gggcagcgat ccgagatcgt cgatctccac atgcaggcgc gtgaagtctt ccaggtcccg
     7921 gctcgaatcg ggagccgggg tgttggcgtc gggcctggca atgggccagg tttctccacc
     7981 ggtctccaga tcgtcctgat cgtggttgac ccagaacacg aaaggctgcg aatccgaggg
     8041 ggcgtcctgt ggcacgcggc tgccgggcag ggtctggatc aagccgtcgc cgttccagtc
     8101 gacgccgata cggacatcgg ccgccgcggc gtgcccggca agcaaagccg tggggagcag
     8161 ccagggcagg aatcgtctca acgtcgccgg tccagagtcg gtcggtgtca cggtttcggt
     8221 cctgcttttc gcgtcattcc tcggtggcac tggtatccat cccggcgagc gcatccagcc
     8281 ggctcaggct gaccaccgcg tacccccgtt ccttcagttt cggtagcagc agctttaagg
     8341 tttctgcggt tctcctgccc cgctcgccca cgtcgtgaag caccaccacg ctgccgggct
     8401 ctatcatccg ctcgacgaag cccgccatgc gggccgaatt atgaatcacg gtgtccagcg
     8461 gtgccaccga ggccagcgcg atccggtagc cccgcgcgcg cgtcagctcg agcaccgcgt
     8521 cgttgtaacg gccggagccc ggccgaaacc aggtgatggg cgcaaacgcg tcgagcgtct
     8581 gcgcggcgcg atggaatttc tctgccatcg cagccggttt aagggagacc gtgacctcgt
     8641 cctcggtcat gtggtggccg atctcgtggc cct
//
LOCUS       DBNHOX010000170         3471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1620277, whole genome shotgun sequence.
ACCESSION   DBNHOX010000170 DBNHOX010000000
VERSION     DBNHOX010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1620277"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="AC16UZ_03650"
     CDS             complement(<1..1208)
                     /locus_tag="AC16UZ_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013707.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR6245808.1"
                     /translation="MVEEKTSYVIRFAGDSGDGIQLQGQQFTTASALAGHDLATMPDF
                     PAEIRAPAGTRYGVSAFQIQFGGDRVTTPGDAPDVLIAFNPAALVVNLSHLRPGALVV
                     VDRQAFTDQRLKRADLTSNPLDDGTLDGFELLDIDISDLTHQAVDALGLGAKDSLRCK
                     NFWALGLVLWMFDQKRDATVSWIEAKFAGNEQVRSANLAALNAGHAFGETAELGPKLR
                     QHAAPEASLASAEYRTLTGAEALALGLVAAGELADTELMFCSYPITPASSLLHQLTGL
                     EDAGVATFQAEDEIAAICSAIGASYAGKLGVTSSSGPGIALKTEAMGLAVAAELPLII
                     VNSQRAGPSTGMPTKTEQSDLYQAVYGRNGDTPLPVVASRSPADCFDTAIEAVRIALR
                     HMTPVILLTDG"
     gene            1383..1757
                     /locus_tag="AC16UZ_03655"
     CDS             1383..1757
                     /locus_tag="AC16UZ_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245809.1"
                     /translation="MKVVVKTDEYTIYQRRDERYAVKDATKAWVNGDAKAAILVQHEL
                     ITAALPAAPLEEPEADAGEPAAEAAAAEEVTGAEESAAEESEPAEAAAPAEEAAAAEV
                     AAEDEAGEPASAEEGEEEPKPE"
     gene            1764..>3471
                     /gene="uvrB"
                     /locus_tag="AC16UZ_03660"
     CDS             1764..>3471
                     /gene="uvrB"
                     /locus_tag="AC16UZ_03660"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR6245810.1"
                     /translation="MVKAVAEFEVVSDYEPAGDQPTAIAGLVEGLVDGLSHQTLLGVT
                     GSGKTYTVANVIEAVQRPTLVLAPNKTLAAQLYGEFRDFFPNNAVEYFVSYYDYYQPE
                     AYVPASDTYIEKDSSINAHIEQMRLSATKALLERRDTIIVASVSAIYGLGDPQSYLRM
                     VLHLDRGDRVDQRYVLQRLAELQYTRNDMAFQRGSYRVRGDVVDIFPAESEKEAVRVE
                     LFDDEIENISVFDPLTGEIVNRVPRYTVFPKTHYVTPRERILAVLEDIKEELQDRLAY
                     LQKHHKLVEAQRLDQRTRFDLEMIRELGYCSGIENYSRYLSGREPGEPPPTLFDYLPK
                     DALLVIDESHVTVPQIGGMYKGDRSRKETLVEYGFRLPSALDNRPLRFDEWERMSPQT
                     IFVSATPGPYEAERSGAVVEQVVRPTGLVDPDVIVRPASTQVDDLFGEIRRTVENRER
                     VLVTTLTKRMAEDLTEYLDEHGVRVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLL
                     REGLDMPEVSLVAILDADKEGFLRAERSLIQTIGRAARNVNGRAILYADHETGSMRKA
                     IGE"
BASE COUNT          636 a         1089 c         1150 g          596 t
ORIGIN      
        1 ccgtcggtca gaagaatcac cggcgtcatg tgccgcagcg cgatgcgcac cgcctcgatg
       61 gcggtatcga agcaatccgc gggggagcgg gaggcgacca ccggcagcgg cgtgtccccg
      121 ttgcgaccgt acacggcctg gtaaaggtcg gactgctcgg ttttggtggg catccccgtg
      181 gatggccccg cccgctggga atttacgatg atcagcggca gctcggcggc caccgccagg
      241 cccatggcct cggtcttcag cgcgattccc ggcccggagc tcgaagtgac gcccagcttg
      301 ccggcgtacg aggcgccgat ggccgagcag atcgccgcga tctcgtcttc ggcctggaag
      361 gtcgccaccc cggcatcctc cagcccggtc agctggtgca gcagcgagga ggccggcgtg
      421 atggggtagg agcagaacat cagctccgta tccgcgagct ccccggcggc caccaggccc
      481 agggccagcg cctcggcgcc ggtgagggtc cggtactcgg ccgacgccag cgaggcttcg
      541 ggcgcggcgt gctgacgcag cttcgggccg agctcggccg tctcgccgaa agcgtggccg
      601 gcgttcagag ctgcgaggtt ggcgcttcgc acttgctcgt taccggcgaa tttcgcctcg
      661 atccaggaga ccgtggcgtc gcgtttctga tcgaacatcc acagcacgag tcccagtgcc
      721 cagaagttct tgcagcgcag tgagtccttg gcgcccagcc cgagagcgtc tacagcctgg
      781 tgggtcagat ccgagatgtc gatgtcgagc agctcgaaac catcgagggt gccgtcgtcg
      841 agggggttgc tcgtcaggtc cgcgcgcttc aggcgctggt cggtgaaggc ctgccggtcg
      901 acgacgacca gcgcgcccgg cctcagatgg gacaggttga ccaccagcgc ggccgggttg
      961 aaggcaatga ggacgtcggg agcatcgccc ggcgtggtga cccggtcgcc gccgaactgg
     1021 atctgaaacg cggacacgcc gtaccgggta ccggccggcg cgcggatctc cgccggaaag
     1081 tcgggcatgg tggctaggtc gtggccggca agagcggagg ccgtggtgaa ctgctggccc
     1141 tgaagctgga tgccgtcacc ggagtccccc gcaaagcgga taacgtagct ggttttttcc
     1201 tcgaccattt gaggctcccg gagcccgccc gcaaaaaaag gtgcggaatt gtacttaatt
     1261 ccgcgcgcaa gtggagatcc aatgggacat aaggttatgt cctgactgcc cctgcaggcg
     1321 ccggatgatt ttgcggttca aatcccttag agtgcgccgc ttgccacact ctggagcctg
     1381 aagtgaaagt cgtagtcaaa accgacgagt acaccattta ccagcgccgc gacgaacgtt
     1441 atgccgtgaa ggacgccacg aaggcctggg tcaatggaga cgcgaaggcc gccatcctcg
     1501 tccagcacga gctgattacg gccgccctgc cggccgcgcc gctcgaagag cccgaagccg
     1561 acgccggcga gccggcggcg gaagccgcag ccgccgaaga ggtcactggg gccgaagagt
     1621 cggccgcgga agagtctgaa cccgccgagg cggctgctcc tgccgaggaa gccgccgcgg
     1681 ccgaggttgc tgcggaagac gaagcgggcg agccggcttc ggccgaagag ggcgaagagg
     1741 aacccaaacc ggaatagcgc tcaatggtca aggccgtcgc ggaatttgag gtcgtttccg
     1801 actatgagcc cgcgggcgat cagccgacgg cgatcgccgg tctcgtcgag ggtctcgtcg
     1861 acggcctgag ccaccagacg ctcctcggcg taaccgggtc aggcaagacg tacaccgtcg
     1921 ccaacgtcat cgaggccgtt cagcggccga cgctcgtcct ggctcccaac aagactctgg
     1981 cggcacagct ctacggcgag tttcgggatt tctttccgaa caatgccgtg gagtactttg
     2041 tttcctacta cgactattac cagccggagg cctacgtccc ggcttccgac acttacatcg
     2101 agaaggactc gtcgatcaac gcgcacatcg agcagatgcg gctgtcggcg accaaggcgc
     2161 tgctggagcg tcgcgacacc atcatcgtgg cgtcggtctc ggccatctac gggctgggtg
     2221 accctcagtc ctacctgcga atggttctgc atctggatcg tggggatcgc gtggaccagc
     2281 gctacgtgct gcagcgcttg gcggagctgc agtacacgcg caacgacatg gcttttcaac
     2341 ggggctccta ccgcgttcgt ggcgacgtcg tggacatatt tcccgcggag tcggagaagg
     2401 aggccgtgcg tgtggagctc ttcgacgacg agatcgaaaa catctccgtc ttcgatcctc
     2461 tgacgggcga gatcgtcaac cgggtgcctc gctacacggt ctttcccaag actcactacg
     2521 tgacgccgcg cgagcgcatt cttgcggtgc tcgaagacat caaggaagag ttgcaggata
     2581 ggctggccta tctgcagaag catcacaagc tggtcgaggc tcagcggctg gaccagcgta
     2641 cgcgcttcga tctggagatg atccgcgagc tgggctactg cagtggtatc gagaactact
     2701 cgcgctacct gtccggccgc gagccgggtg agccgccgcc gaccctgttc gattacctgc
     2761 ccaaggacgc gttgctggtc atcgacgaaa gccacgtgac cgttccccag ataggtggca
     2821 tgtacaaagg tgatcgttcc agaaaggaga cgctggtgga gtacggcttc cggcttcctt
     2881 ccgctctgga caaccggccg ctgcgattcg acgagtggga gcgcatgagc ccccagacca
     2941 tcttcgtctc cgccaccccg ggaccttacg aggcggaacg gtccggcgcc gtcgtcgagc
     3001 aggtggtgcg tccgaccggc ctggtggatc cggacgtgat cgtgcgcccc gcgtcgacgc
     3061 aggttgacga cctgttcggc gaaattcgcc gcacggttga gaacagggag cgcgtgctcg
     3121 tcaccaccct gaccaagcga atggcagagg atctcaccga gtacctggac gagcacggcg
     3181 tccgggtgcg ctatctgcac tcggacatcg atacggtgga acgcatggaa atcatccgcg
     3241 atctgcgcct cggcgagttc gatgttctgg tcggcatcaa tctgcttcgg gagggcctgg
     3301 acatgcccga ggtctccctc gtggcgattc tcgacgccga caaggaaggc tttctgcgcg
     3361 ccgaacgttc gctgattcaa accatcggtc gcgccgcgcg caacgtcaat ggccgggcga
     3421 ttctctacgc cgatcatgaa accggttcca tgcgcaaggc catcggagag a
//
LOCUS       DBNHOX010000171         4039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_13213, whole genome shotgun sequence.
ACCESSION   DBNHOX010000171 DBNHOX010000000
VERSION     DBNHOX010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_13213"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="AC16UZ_03665"
     CDS             complement(<1..332)
                     /locus_tag="AC16UZ_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent monooxygenase"
                     /protein_id="HIR6245811.1"
                     /translation="MHEREFVERIRGLVPMLEAHAGEAERDRKPVDSVMAAVEETGAY
                     RFFVPKRYGGWEFGLDTFMDVGLLLGEGCVSTAWVVTFCMEHNWLLALYNKTAQDDIF
                     GKQPYIIAP"
     gene            complement(344..853)
                     /locus_tag="AC16UZ_03670"
     CDS             complement(344..853)
                     /locus_tag="AC16UZ_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6245812.1"
                     /translation="MIGQSRTLQSSNEPVGVMADLVRALDWFDNGLQNVLASLGYKPL
                     HRTQSMIMVHVASGVESPADIARSMGLSRQNVHHMAKALIADGLIEQAPDPADPRRTL
                     YRLAGSAAEIRRAALETLAQLELVLAGRIGLRSLHALRGALGKDWGDAIAGGEALAAE
                     LDGHDVLAN"
     gene            1093..2208
                     /locus_tag="AC16UZ_03675"
     CDS             1093..2208
                     /locus_tag="AC16UZ_03675"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6245813.1"
                     /translation="MASWQRQFVSTTSPTGTRNGAGFYPCQGLYYAPTGSRPRTALIA
                     THYNVDFSEHYLGEFMAARGYGFLGWNTRFRGAEAFFLLEHALVDIGTGVRWLKEEAG
                     VERVVILGNSGGGSLMGAYQSQALGVTMTATPGLSLPDAIHELIPADLYISLCAHLGR
                     PEVLTDWFDPSVTDETDPTSIDPFLNMYEPANGPPYAPGFVKRYRAAQRERNDRITRW
                     CHAELARLAELGMFDRAFNLYRTWADLRLMDGSLDPSEREVGRCYAGDPKTANFSPRG
                     IGLTNTLRTWLSMWSLRDSHCRGAPHLNRITQPALVIQSLADTGVFPGDARAIFDALG
                     SKDKHLEMITGDHYLENPRDARDGVADMIADWLASHT"
     gene            2265..3968
                     /locus_tag="AC16UZ_03680"
     CDS             2265..3968
                     /locus_tag="AC16UZ_03680"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6245814.1"
                     /translation="MLLCATNAGAATCTNATPGEAQLYWGDLHVHTAYSLDAYAFGAI
                     AAPKEAYAFGRGQPLRLANGEMVAIDRPLDFMAVTDHAETYDVMYICTDPLYRDDAYC
                     RAIRSGPEPGNARTVFNDYLLPLVSDQPPQMAAVCDEDGVDCRAASIGQWRRAQRAAN
                     AANDPCTFTALIGYEWTASPGGHHWHRNVIFRSDRVPDRAADYVHFPEVTALWRELDT
                     HCRSEDGCEVLTIPHNINWADGGRTFDIEHASEADLAARARFERLAEIHQEKGNSECL
                     PAHRDDPGSDCAFELLTDNAAKARLTGPEDISPEDAWRRMRSTYYRGLLARGLAAYAS
                     SESGLNPFMLGAIGSTDTHFGTPGLVSEAGYPGGIASLWLDGEERLTLPNYNPGGLVA
                     VWAQENTRAAIFDSLQARSAYATSGTRIKLRFGATEHDACENSKVNYDTFMGETLTTG
                     LPSDNTAGPVFTVEAGRDEAPLGTVQIIKGELREDGVVETVHTVADFPDGRDAVCISW
                     QDEAFTAAAPAYWYARVLELPAPRWTKLLCEATGSCDRFPDADRNIRERAWSSPIWHL
                     P"
BASE COUNT          724 a         1408 c         1239 g          668 t
ORIGIN      
        1 ggcgcgatga tgtagggctg cttgccgaag atgtcgtcct gggcggtctt gttgtagagc
       61 gcgagcagcc agttgtgctc catgcagaag gtcaccaccc aggcggtgga tacgcagcct
      121 tcgccaagca ggaggccgac gtccatgaac gtgtccaacc cgaactccca gccgccgtag
      181 cgtttcggca cgaagaaccg gtacgcgccg gtttcctcga ccgccgccat cacggaatcc
      241 acgggcttgc gatcgcgctc ggcctcgccg gcatgggcct ccagcatcgg caccagcccg
      301 cggatgcgct cgacaaattc ccgttcgtgc atacccgcct acctcagtta gccaacacgt
      361 cgtgcccgtc gagctctgcc gcgagcgctt caccgccggc aatcgcgtcg ccccagtctt
      421 tgccgagggc gccccgaagc gcatggaggc tgcggagccc aatgcgtcca gcgagcacga
      481 gctcgagctg agccagagtc tccaaagctg cgcggcggat ctccgcggcg gagcccgcca
      541 gccgataaag tgtgcgccgc gggtccgccg ggtccggcgc ctgttcgatg agcccgtcgg
      601 cgatgagcgc tttggccatg tgatgcacgt tctggcggct caggcccatc gatcgggcga
      661 tgtctgccgg cgactcgacc ccgctggcga cgtgaaccat gatcatggac tgggtccggt
      721 gcagcggctt gtagcccagc gaggcgagca cgttctgaag gccgttgtcg aaccagtcga
      781 gcgcgcgcac cagatcggcc atcacgccaa ccggttcgtt gctgctttgc agcgttcgcg
      841 actgcccaat catcgttaac cccgaatttc cggatctgat cttcccggtg ccgacgtcgc
      901 catcgagcac tttctcacga ttcgccgagg gcgaatcggg ccgcgcgcaa agcatccagc
      961 ccgcccagtt agcgtcaaat gatcttacac agaaccacgg gtcccgaaca gcgtcgccag
     1021 agccaacgcc gctttgaaag ctccggatat atcccgtaat gtcggtgcct caggcttccg
     1081 gggggatcgc caatggccag ttggcagcgc cagttcgttt ccacgacctc accgaccggc
     1141 acccgcaacg gcgccggttt ttatccctgt cagggtctgt actacgcccc gacgggctcg
     1201 cggccccgga cggccctgat cgcgacgcac tacaacgtgg acttcagcga gcactacctg
     1261 ggcgagttca tggcagcacg cgggtacggc ttcctgggct ggaatacccg tttccgcgga
     1321 gcggaagcct tcttcctcct ggaacacgct ctcgtcgaca tcggaaccgg cgtgcgctgg
     1381 ctgaaagaag aagccggcgt ggagcgggtg gtcatcctcg gcaactccgg cggcggctcc
     1441 ctgatgggcg cctaccagtc ccaggcactc ggcgtaacca tgacggcgac ccccggtctg
     1501 tccttgcccg atgcgatcca cgagctcatc ccggccgatc tctacatctc tctgtgtgcc
     1561 cacctcggcc gtccggaagt gctcaccgac tggttcgacc cttccgtgac ggacgagacc
     1621 gatccgacga gcatcgaccc gttcctgaac atgtacgaac cggccaacgg gccaccctac
     1681 gcgcccggat tcgtgaagcg ctatcgggca gcgcagcggg aacgcaacga ccgaattacc
     1741 cgctggtgcc acgcagagct ggcaaggctc gccgagctcg gcatgttcga ccgcgctttc
     1801 aacctctacc gcacctgggc cgatctccgg ttgatggacg gctctctgga cccatcggaa
     1861 agggaagtcg gccgctgcta cgccggcgac cccaaaacgg ccaacttcag ccctcggggt
     1921 attggcctga ccaacacgct acggacgtgg ctgtccatgt ggagcttaag ggactcccat
     1981 tgccggggag cgccgcacct gaaccgcatc acccagccgg ccctcgtgat ccagagtctg
     2041 gccgacaccg gtgtctttcc gggggatgcc agggcgatat tcgatgcgct ggggagcaag
     2101 gataaacacc tggagatgat caccggcgat cactacctgg agaatccgcg cgatgcacgc
     2161 gacggcgttg ccgacatgat cgccgactgg ctcgcctcgc acacgtgagc tcagttgccc
     2221 gctgtctgtc accgtacctg gggcctgcgc tggcggcatg gggtttgctc ctctgcgcga
     2281 ccaacgcggg cgccgccacc tgcaccaacg caaccccggg ggaggctcag ctgtactggg
     2341 gcgatctgca cgttcacacg gcctactccc tcgacgccta cgcatttggc gccatcgcag
     2401 cgcccaaaga ggcctacgcg ttcggccgcg gtcagcctct gcgtctggcc aacggggaga
     2461 tggtggccat cgatcgtcca ctggacttca tggcagtcac cgatcacgca gagacctatg
     2521 atgtgatgta catctgcacc gatcccctct accgggatga cgcctactgt cgggccattc
     2581 gcagcggccc cgaacccggc aacgccagaa ccgtcttcaa cgactacctt ctgccgctgg
     2641 tcagcgacca gcctccgcaa atggccgcag tctgcgacga agacggcgtc gattgccgcg
     2701 ctgccagcat cggtcagtgg cgacgcgcgc agcgcgcggc caacgcggcc aacgatccct
     2761 gcactttcac cgccctgatc ggctacgaat ggaccgcctc acccggcggc caccattggc
     2821 accgcaacgt gatcttccgc agtgaccggg tccccgatcg cgccgccgac tacgtgcatt
     2881 ttcccgaggt caccgccctg tggcgggaat tggacaccca ctgtcgaagc gaggacggct
     2941 gcgaggtgct caccattccc cacaacatca attgggccga cggtggccgc actttcgaca
     3001 tcgagcacgc gagcgaagcc gatctggcgg cccgggcgcg tttcgaacgg ctggcggaaa
     3061 ttcaccagga gaagggcaac agcgagtgtc tgcccgccca ccgggatgat ccaggcagcg
     3121 actgcgcgtt cgagctcctg accgacaacg ccgccaaggc gcgactgacc ggccccgagg
     3181 acatctcccc cgaagacgcc tggcgacgca tgcgctcgac ctactatcgc ggcctgctcg
     3241 cccggggcct agcggcctac gcgagcagcg agtccggact caaccccttc atgctgggcg
     3301 ccataggctc taccgacacc cacttcggca ccccgggcct ggtgagtgag gccggctatc
     3361 ctggcggcat cgcctccctg tggctggacg gcgaggaacg cctgacgctg ccgaactaca
     3421 atccgggcgg gctggtggcc gtgtgggcac aggagaatac ccgcgccgcc attttcgatt
     3481 ccctgcaggc gcgcagcgcc tacgccacca gcggcactag gatcaagctg cgcttcggcg
     3541 cgaccgagca cgacgcctgc gagaattcca aggtgaacta cgatacgttc atgggcgaga
     3601 ccctgacgac cgggctcccg tccgacaata ccgccgggcc cgtgttcacc gttgaggccg
     3661 gccgcgacga ggcgccgctg ggaaccgtgc agatcatcaa gggcgagctt cgcgaggatg
     3721 gtgtggtgga aaccgtccac accgtggcgg actttccgga cggccgcgac gcggtctgca
     3781 tttcgtggca ggacgaggca tttaccgccg ccgcgcccgc ctactggtac gcgcgcgtcc
     3841 tggagttgcc cgcgccacgc tggaccaagc tgctctgcga agctaccggg tcgtgcgacc
     3901 gcttccccga tgcggatcgg aacatccggg agcgggcatg gtcctcaccc atctggcacc
     3961 tgccatgaaa cccgaggaaa gcgccgtcat tccgaatacg cgagacaaca cgcaacggag
     4021 atccgaggag ccctgatga
//
LOCUS       DBNHOX010000172         1736 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_335038, whole genome shotgun sequence.
ACCESSION   DBNHOX010000172 DBNHOX010000000
VERSION     DBNHOX010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_335038"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /gene="trpB"
                     /locus_tag="AC16UZ_03685"
     CDS             complement(<1..1055)
                     /gene="trpB"
                     /locus_tag="AC16UZ_03685"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR6245815.1"
                     /translation="MSEGKGFNKYDQPVGGHFGQHGGRFVAETLMHALDELEALYERA
                     RKDPEFQAELAHDLEHFVGRPTPLYHAARLSRDLGGAQIYLKREDLNHTGAHKINNTV
                     GQALLAKRMGKPRVIAETGAGQHGVATATVAARLGLECCVYMGAEDVKRQSLNVYRMK
                     LLGAEVVPVNSGSRTLKDALNEAMRDWVTNVDTTHYIIGTVAGPHPYPMLVRDFQAVI
                     GQEARAQSLSMLGRLPDVLVACVGGGSNAIGLFYPFVDDVDVRMVGVEAGGLGLETGR
                     HAAPLSAGRVGVLHGNRTYLMEDDDGQIIETHSISAGLDYPGVGPEHSFLKDVGRAEY
                     DTVTDDEAMAGFRLLNRT"
     gene            complement(1033..1668)
                     /locus_tag="AC16UZ_03690"
     CDS             complement(1033..1668)
                     /locus_tag="AC16UZ_03690"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR6245816.1"
                     /translation="MPTKVKICGIARPEDARCVADAGADALGLIFVRQSPRFVSTGLA
                     REIAGEVAGSLSLIGLFVDADAAEVEAVLRDVDLDVLQFHGDEPGSYCEAFGVNYMKA
                     TRVSGPVDVAGLEAEYPGACCLLLDAYVPGVAGGTGVRLDLACWPEAGAKPLVLAGGL
                     TPENVADAISRVRPFGVDVSSGVEGGIKGRKDPERVRKFVSEVRRVGREGV"
BASE COUNT          306 a          590 c          556 g          284 t
ORIGIN      
        1 gtccggttca gcaggcgaaa gccggccatg gcttcgtcgt ccgtgacggt atcgtactcg
       61 gcgcggccaa cgtccttgag gaacgaatgc tccggcccga cgccggggta gtcgagcccg
      121 gccgatatgg aatgcgtttc gatgatctga ccatcatcgt cttccatgag gtaggtgcgg
      181 ttgccgtgga gcacgcccac ccgcccggcg ctcaggggcg ccgcgtgcct gccggtctcg
      241 agacccaagc cgcccgcttc cacaccgacc atgcgtacgt cgacgtcgtc gacgaacggg
      301 tagaaaagac caatggcgtt ggagccgccc cccacgcagg ccacgaggac gtcgggcagc
      361 cggccgagca tgctgagaga ctgggcccgc gcttcctgcc cgatcaccgc ctggaagtca
      421 cggaccagca tgggataggg atggggtccg gccaccgtgc cgatgatgta gtgggtcgta
      481 tcgacgttgg tcacccaatc ccgcatggcc tcattgagcg catccttcag ggtgcgcgag
      541 ccggagttga cggggaccac ctccgccccg agcagcttca tccgatagac gttcaggctc
      601 tgccttttga catcttcggc ccccatgtag acgcagcatt ccaggccgag acgggcagcg
      661 acggtcgccg tcgcgacgcc gtgctggcca gcgccggtct cggcgatgac gcgaggcttg
      721 cccatgcgct tggcgagaag cgcctggccg acggtgttgt tgatcttgtg ggcgccggta
      781 tggttgaggt cctcgcgctt gaggtagatc tgcgccccac ccagatcccg ggacagacgc
      841 gcggcatgat agagaggggt tggccgtcca acgaagtgct cgaggtcgtg cgcgagctcg
      901 gcctgaaact cgggatcctt gcgtgcacgc tcgtaaaggg cctccagctc gtccagggcg
      961 tgcatgagcg tctcggcgac gaagcggccg ccgtgctggc cgaagtgacc gcccacgggc
     1021 tggtcgtact tgttaaaccc cttcccttcc gacacggcgc acctcgctga caaattttct
     1081 gacccgctcc gggtccttgc ggcccttgat cccgccctcg acgccgctgc tgacgtccac
     1141 accgaacggc cgcacacggc tgatggcatc ggccacgttc tccggtgtca ggccaccggc
     1201 cagaaccagc ggcttggcac cagcttccgg ccagcaggct agatccaggc gtacgcccgt
     1261 gccgccggca acccccggga cataggcgtc cagtagcagg cagcaggcgc ccggatactc
     1321 ggcctcgagg ccggcgacgt ccacggggcc gctcacacgc gttgccttca tgtaattcac
     1381 cccgaacgcc tcgcaatagg atccgggctc gtctccgtga aactgaagca cgtccaggtc
     1441 aacgtcccga aggacggctt ccacctcagc agcatccgcg tcaacgaaaa gcccgatgag
     1501 gctgagcgac ccggccacct cccctgcgat ctcacgagcg agcccggtgg aaacgaagcg
     1561 agggctctgc cgcacgaaga tcaacccaag ggcgtcggcg ccggcgtccg caacgcaacg
     1621 tgcgtcctcg ggccgcgcaa tcccacaaat ttttaccttg gtcggcacga ccggatccgg
     1681 gggaaaaggg gcgtagtcta gcggagacgt aacgggcctt ctagacgcgt gcgagc
//
LOCUS       DBNHOX010000173         9560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1234511, whole genome shotgun sequence.
ACCESSION   DBNHOX010000173 DBNHOX010000000
VERSION     DBNHOX010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9560
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1234511"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="AC16UZ_03695"
     CDS             complement(<1..686)
                     /locus_tag="AC16UZ_03695"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HIR6245817.1"
                     /translation="MIDDYSDRHHRFEGCFNFRDIGGYPTTEGRTVRWGRYYRAGRQD
                     RMSEADVARARDLGIATQIDLRKPDEVREQGRGPLEEMGVAYHHIPVIPEGGSDALSR
                     LVGDTGISGRRYLGYLEFGPEPWLAMFEIFADAGRHPIVLHCTAGKDRTGVSTAFLLS
                     VLGVDRALIEADYILTNRDVGRQADFIERTVGYPEGMDRAAMVHAAGVPETAIGDFLD
                     GLEARWGGPLG"
     gene            complement(988..2307)
                     /locus_tag="AC16UZ_03700"
     CDS             complement(988..2307)
                     /locus_tag="AC16UZ_03700"
                     /inference="COORDINATES: protein motif:HMM:NF016873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HIR6245818.1"
                     /translation="MRSLKDLLVVIPHSGIRIPPEIPSDSLTEEFPALLANVDWHTDR
                     LYDFTDLLENQQIVFPYCSLLLEANRDPELLDESIPLRDVNGKAVYRPGCEPSLDLRR
                     HLSYKYLRGFHQKISASISAGAEFLLDGHSTVVARGVAANQIDLMNFQHTQRDGEPKL
                     YAPAIYAETYAEALQKRLPEVHVTVNASEYYQVHGHVCAAHSVNGIGRAGQLAPALIQ
                     ETSQGLYMHPDGTPDVLAVDHLRRVFAEALRTTLDTVRRFTAPAQIIDLHGQRQTFDF
                     DCGVKALQMVLAYYGVEEREDKLLKALAADPELGTPLARMIEFAESRGFEVKAGTGWS
                     LDDVKHYLDRNCPVIVLVQAWADGYLSLAEWRRNFDDGHYVVVVGYDRQNLYFEDPAS
                     FHRTWLKENEFLARWHDQDPVTGEKLLQAGMVLLGREPVGKGLRPMY"
     gene            2783..3970
                     /locus_tag="AC16UZ_03705"
     CDS             2783..3970
                     /locus_tag="AC16UZ_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HIR6245819.1"
                     /translation="MQRRAKVVRLRTAGRGNPRTREFRIRHFPDVWDAEWNDWRWQSR
                     HRFKNLAQIERVLELSDDERAALTVGGMMLPVGITPYYMSLLDPVDPQHALRRTVIPS
                     QNEFLRLPGEADDPLGEDAHSPVPGLVHRYPDRVLMLPIDFCSTYCRYCTRSRVVGHG
                     ELSPQTSRLEAMFDYLRGATQVRDVLVSGGDALALGEDNLDYILSNLRAIEHIEFVRI
                     GTKMPAVLPQRITRDLTRVLRKYHPLWMSLHFLHPEECTPESTQACERLADAGIPLGS
                     QTVLLKGVNDDVGVMTRLVHGLLKMRVRPYYLYQCDPISGSAHFRTTVEKGLEIIEGL
                     RGHTTGYAVPTYVIDAPGGGGKIPVQPNYVLGRDVDELKLRNFEGRVFSYPDPQQPGA
                     GTN"
     gene            3973..4986
                     /locus_tag="AC16UZ_03710"
     CDS             3973..4986
                     /locus_tag="AC16UZ_03710"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR6245820.1"
                     /translation="MRIGITYDLRSAYLAAGYSEEETAEFDRDDTVDAIEAALRQLGH
                     ETVRIGCARELIEHLAAGQRWDLVFNICEGLSGPGRESQVPAILEVYGIPVTFADTAV
                     MALSLNKAWAKTVVADAGVATPQHAVIARAADVATVALEPPLFVKPIGEGTGKGTSAA
                     SVVMSAAELERQSLALLERYRQPVLVETYLPGREFTVGLLGTGPNARVLGTLEIELLA
                     TAEANVYSYLNKEECESRVLYRLVDGRADPVVARAEAAALGAWRALGARDGGRIDLRA
                     DSAGEPNFIEANPLAGLHPAHSDLPMLATAVGMPYAELIRAIVDSASERLAMDATEMN
                     RCA"
     gene            4977..5963
                     /locus_tag="AC16UZ_03715"
     CDS             4977..5963
                     /locus_tag="AC16UZ_03715"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR6245821.1"
                     /translation="MRVAIAYNKPGDAAGSAEADVLEQVEAVETALVGAGHQVCALPC
                     TLDLAGFDGALAKQRPDVVFNLVEALGGTDRLAVLVTLLLDARGVPYTGAGSRALELS
                     ADKVGAKERMRRLGLPTPDWYVCEKTQVDFPGAGCYIVKARFEHASLGLDDQSVVQVA
                     DAGQLTRRIRARGKHSGAQQFAERYVAGREFNVALLASETGPAQVLPPAEIDFSAFAP
                     GKPRIVGHDAKWSSRAFEYHATPRRFDFPAADARLLDLLGTLGMQVWECFAMRGCARV
                     DFRVDEDGRPWILEINPNPCLSPDAGFAAALDAAGIPAEEMVGRLVRTAATD"
     gene            5966..6505
                     /locus_tag="AC16UZ_03720"
     CDS             5966..6505
                     /locus_tag="AC16UZ_03720"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6245822.1"
                     /translation="MTSPCKEHPPGDAWVHLREPAARDRGPVASVLVSTGFFSDAEVA
                     VALEVFDAALQGKDSGYEHLVAESAGRVIGYVCWGGPIEMTLSSFDLYWIAVDAAARG
                     RGVGHRLLAAAESLAAASGCAQLFAETSGRADYAPTHSFYESCGYLRAAELADFYAPG
                     DAKVFYVKRLTGGNQAPPE"
     gene            complement(6540..8120)
                     /gene="groL"
                     /locus_tag="AC16UZ_03725"
     CDS             complement(6540..8120)
                     /gene="groL"
                     /locus_tag="AC16UZ_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155568.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR6245823.1"
                     /translation="MSAKQIVFAHDARARLNRGVDTLARAVNATLGPRGRNVLLSKTW
                     GAPTVTKDGVTVAKDIELKDRFEDMGARIVRQAATRTADEAGDGTTTATVIAHSLFHW
                     GRRAIAAGFSPVEIKRGIDLGVGLMTDALDAMAVECEAGDDIRKVATISANGDEAIGE
                     LIAQAMEQVGREGVITVKDGTGIDDQLEVSEGMQFDQGYLSPYFVTSTETYAAELDRP
                     VILLRDKKISSSRELLPLLEAVARANRSLLIVAETVEADALATLVVNRLRGRLKVAAV
                     KAPGFGDRRKAMLQDLAILTGGKVISSDAGFSLEKVALEDLGEAQRVLLTKGDTTLIG
                     GRGGTDAIAGRISQIRREIEESKSEYDREKLQERLAKLAGGVAVINVGGATEVAMKER
                     KMRVDDALAATRAAVEEGVVPGGGVALLRASAELAKAAPRNQDQASGVDALRRAAEAP
                     LRQIVANAGLQPGVVLRKIQSEQGHFGFNAQTETFGDMLEMGILDPCKVVKSALHNAA
                     SVAGMMMSTEALVADAPR"
     gene            complement(8311..8868)
                     /locus_tag="AC16UZ_03730"
     CDS             complement(8311..8868)
                     /locus_tag="AC16UZ_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6245824.1"
                     /translation="MSHESQRRVSLADLSDEALAASARHQLPADTSAYDELVRRHYES
                     VRRLATAQLGAPDEADALAQDVMLRVFSGLKNFRGDAKFSTWLFRIVENLARTRVRQQ
                     IREQDKREALGMAQHEREEDAPDQSDFQRLLAVLSPQERALLALRFVEDMELHEIAAV
                     LGLSLSATKMRYYRTLDKLKDHIPG"
     gene            9094..>9560
                     /locus_tag="AC16UZ_03735"
     CDS             9094..>9560
                     /locus_tag="AC16UZ_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR6245825.1"
                     /translation="MTMPTMGGKRPENRVSLQLLQRILVDASSAHTVEEQVERLVSQV
                     RDAMAAGACSLYQRFDDTLRMVANCGFAPESVGRIALPMGEGLVGRIARDRMSLNLAD
                     ASAHPDFRLFPQSGEEAFPAFLGVPIIHLGAVNGVLIVQDDRPSAFSEDEVSFL"
BASE COUNT         1584 a         3137 c         3115 g         1724 t
ORIGIN      
        1 cccagcggtc cgccccagcg cgcctcgagg ccgtccagga agtcgccgat ggcagtctcg
       61 ggcacccctg cggcgtgcac catcgctgcc cgatccatgc cctccgggta gccgacggtg
      121 cgctcgatga aatccgcctg ccggcccacg tcgcggttgg tgagaatgta gtcggcctcg
      181 atcagagcgc ggtccacgcc cagcacggat aggagaaagg cggtggagac ccccgtgcgg
      241 tccttgcctg cagtgcagtg cagcacgatg gggtgacggc cggcgtcggc gaatatctcg
      301 aacatcgcaa gccacggctc gggcccgaac tccagatagc cgaggtagcg acgtccggag
      361 atgccggtgt cccccaccag gcggctcaat gcatcgctgc cgccctccgg gatgacgggg
      421 atgtggtggt aggcaacgcc catctcctcc aaaggacccc ggccctgctc tcggacctcg
      481 tcgggcttgc gcaggtcgat ctgcgtggcg atgcccagat cgcgagccct ggcaacatcg
      541 gcttcggaca tgcggtcctg gcgacccgcc cgatagtagc ggccccatcg caccgtgcga
      601 ccctcggtcg tcgggtagcc gccgatgtcg cgaaagttga agcagccttc gaatcggtga
      661 tgccggtcgg aatagtcatc gatcatatgt tgaagttcat tcggaaagcg cgtccatgtc
      721 aaacggaccg tgtcgggaag accgtttcga tcagaccgtc tctgaccgac ccggcccgac
      781 gttgtcgtca gtgagacagc ggcctggtga aaaagttgat ggtcccggcg tctggaacgt
      841 cgacgaagaa cgtgccaccg gtcgccgaat gccggcaacg ttcaacagga aaaagtagtc
      901 gtgggcgtgc tcgtgcttgt cgatccgggc gcctgcggtg gagatgcctg gcgcggctgc
      961 cttcggtgat gagccaaagc ggtctggtca gtacatgggc cgcaggccct tgcccaccgg
     1021 ctccctgccc agcagcacca tgccggcctg gaggagcttt tccccggtaa ccggatcctg
     1081 gtcgtgccag cgggcgagga attcgttttc cttcagccac gtgcggtgaa acgaggcggg
     1141 gtcctcgaag tagagattct gacggtcgta gcccaccacc acgacgtagt gcccgtcgtc
     1201 gaagttgcgt cgccattcgg ccagcgagag gtagccgtcg gcccaggctt ggaccaggac
     1261 gatgaccggg cagttgcggt cgagatagtg cttcacgtca tccagcgacc agcccgttcc
     1321 ggccttgacc tcgaagcccc tggactcagc gaactcgatc atcctggcca gcggcgtgcc
     1381 gagttcgggg tcggcggcga gcgctttgag cagtttgtct tcgcgctcct cgacgccgta
     1441 gtaggccagc accatctgca gcgctttcac cccgcagtcg aaatcgaacg tctggcgttg
     1501 gccatgcaga tcgatgatct gcgccggcgc tgtgaaccgt cggacggtgt ccagcgttgt
     1561 gcgcagggcc tcggcaaaga cccggcgcag gtgatctacc gccagcacgt ccggcgtccc
     1621 gtcggggtgc atgtaaaggc cctgagaagt ttcctgaatc agcgcgggcg ccagctgccc
     1681 ggctctgccg ataccgttga ccgagtgggc ggcacagacg tgaccgtgaa cctgatagta
     1741 ctccgaggcg ttgacggtga cgtggacctc gggcagccgt ttctgcaggg cctcggcgta
     1801 tgtctcggcg tagatcgccg gcgcgtacag cttcggctcg ccgtcgcgct gcgtatgctg
     1861 aaagttcatg agatcgatct gatttgctgc cacgcccctg gcgaccacgg tggagtggcc
     1921 gtccaggaga aactcggcgc ccgcggagat gctcgcgctg atcttctgat gaaagccgcg
     1981 caggtacttg taggacaggt ggcggcgcag gtccaggctc ggctcacagc cgggccggta
     2041 gacggctttg ccgttgacgt cgcgcagggg tatcgactcg tccagcaact cggggtcgcg
     2101 attggcctcg agcaggaggc tgcagtacgg gaagacgatc tgctgattct cgagcaggtc
     2161 ggtaaagtcg tacaggcggt cggtgtgcca gtccacgttg gcaagcagtg ccggaaactc
     2221 ttcggtgagg ctatcactcg gtatctccgg cggaatgcgt atcccgctat gggggatgac
     2281 caccagaaga tctttcaggc tgcgcatgcg cgacccttga aatgtctgaa gaacgtcata
     2341 gaaatcccgg tccgcagaca ttgcgtggga ccggcgggga tccgcagtaa gcgccaaaaa
     2401 tccggcaaat acaatgggct cgcggcggcg tttgcctaca tgaaaaaaag tccggttgat
     2461 gtgatgcacg gctattgcat tcaaccccgt ttgccttaac ttacgcgccg ttcggcagct
     2521 ccgaggggct gtcgtttttc gttggaccgc cacaggagat ccaaggcgca tgaaaaagtc
     2581 ccccccaccg gtcgaagagg agcctccggg aggtattcac ggccagaatc tagccctcag
     2641 cctgcatcca ccccattcga atacccgatc caacaagcca atcacctccc gtaccgcgac
     2701 agccgacgac gcgcgtgtca tcaaagtcat ccctgcaaag tccaccgact cgacatccgt
     2761 tgcatcgaag ggcgtcgagt ccgtgcagcg tcgggcgaag gtagtccggc tgcggaccgc
     2821 agggcgcggc aacccccgca cacgcgagtt ccggatccgc catttcccgg acgtttggga
     2881 tgcggagtgg aacgactggc gctggcagtc ccggcaccgc ttcaagaatc tcgcccagat
     2941 cgagcgcgtg ctcgaactgt ccgacgatga gcgcgcagcg ctcaccgttg gcggcatgat
     3001 gctgcccgtg gggatcacgc cctactacat gagtctgctc gaccccgttg atccgcagca
     3061 tgccctgcgg cggacggtca taccctcgca gaacgaattt cttcgcctgc cgggggaagc
     3121 cgacgacccg ctcggggagg acgcccacag tccggtgccg gggctcgtgc accgttaccc
     3181 cgaccgggtg ctgatgctgc caatcgattt ctgttccacc tactgccgct actgcactcg
     3241 ctcgcgggta gtaggccatg gggagctctc cccccagaca tcgcgcctgg aagccatgtt
     3301 cgattacctg cgaggcgcga cccaggttcg cgacgtgctc gtctccggcg gcgacgcgct
     3361 tgccctcggc gaggacaacc tcgactacat cctttccaac ctgcgggcca tagagcacat
     3421 cgaattcgtc cgcatcggca ccaagatgcc ggccgtgctc ccgcaacgaa tcacccggga
     3481 tctgaccagg gtgcttcgca agtaccaccc gctctggatg agcctgcact tcctgcaccc
     3541 ggaggaatgc acgccggagt ccacccaggc ctgcgagcgg ctggccgatg ccggtatacc
     3601 gctcggctcc cagaccgttc tgctcaaggg cgtgaacgac gacgtgggcg tgatgacgcg
     3661 cctcgtgcac ggcctcctga agatgcgggt gcggccctac tacctgtacc agtgtgaccc
     3721 gatcagtggt tcggcccact tccgcacgac cgttgagaag ggcctggaga tcatcgaagg
     3781 gctgcgcggc cacaccacgg gttacgccgt gccgacctac gtcatcgatg cgcccggcgg
     3841 tgggggcaag attccggttc agccgaacta cgtgctcggc agagacgtcg acgagctcaa
     3901 actccgcaat ttcgagggac gggtcttcag ctatccggat ccccagcaac ctggcgccgg
     3961 gaccaactga acatgcgcat cggtatcacc tacgatctgc gttccgcgta cctcgccgcc
     4021 ggctactcgg aggaggaaac ggcggagttc gaccgcgacg acaccgtcga cgccatcgag
     4081 gcggcgttgc gccagctcgg gcacgagacg gtgcgcatcg gctgcgcccg cgagctcatc
     4141 gagcaccttg ccgcgggaca gcgttgggat ctggtgttca acatctgcga ggggctcagc
     4201 ggtcccgggc gggagagcca ggtgccagcc attctggagg tctacggcat acccgtgacg
     4261 tttgccgata cagccgtcat ggctctgagc ctgaacaagg cctgggccaa gacggtggtg
     4321 gccgacgccg gtgtggcgac cccgcagcat gccgtcatcg cccgggccgc tgacgttgcc
     4381 acggtcgcgc tcgagccgcc cctgttcgtc aagccgatcg gggagggcac cggcaagggc
     4441 acaagcgccg cttcggtcgt catgagcgcc gccgagcttg aacggcagag cctggccctg
     4501 ctcgagcgct accggcagcc cgtgctggtc gagacgtacc tccctggcag agagttcacc
     4561 gtcggccttc tgggcactgg cccgaatgcc agggtgctcg ggactctcga gatcgagctc
     4621 ctggcaactg ccgaggcgaa cgtctactcg tacctcaaca aggaggagtg cgagtcccgt
     4681 gtgctgtacc gactcgtcga tggtcgtgcc gatcccgtcg ttgctcgggc cgaggccgcg
     4741 gcgctcggcg cctggcgggc cctcggggcc cgggacggag ggcgcatcga ccttcgcgcg
     4801 gactcggcgg gggaacccaa tttcatcgag gcaaaccccc tggcagggct gcatcccgct
     4861 cattccgatc tgcccatgct ggctaccgcg gtgggaatgc cttatgcaga gctcattcgt
     4921 gccatcgtcg actcggcttc ggaacgtctt gccatggacg ctacggaaat gaaccgttgc
     4981 gcgtagccat cgcttacaac aagccgggag acgctgcggg cagcgcggaa gccgacgtgc
     5041 tcgagcaggt cgaagcggtt gagactgccc tcgtgggcgc cggtcaccag gtctgcgcgt
     5101 tgccctgcac gctggatctc gccggattcg atggtgccct ggcaaagcag cggcctgacg
     5161 tcgtcttcaa tctggtcgag gccctgggcg gaaccgaccg cctggccgtg ctggtgacgc
     5221 tgctcctcga cgcccgcggt gtcccctaca ccggagcggg atcgcgggcg ctggagctca
     5281 gcgccgataa ggtcggcgcc aaggaacgaa tgcgcaggct cggcctgccg acgccggact
     5341 ggtacgtctg tgagaagacc caggtggatt tccctggggc agggtgctac atcgtcaagg
     5401 cacgctttga gcacgcctcc cttggcctgg acgaccagtc ggtcgttcag gttgctgatg
     5461 ccgggcagct caccaggcgc atccgcgcac gcggaaagca cagtggcgcg cagcagttcg
     5521 ccgaacgcta cgttgccggg cgtgagttca acgtggcgct tctggcttcg gaaaccggcc
     5581 ccgcccaggt gctgcctccg gcggagatcg acttctcggc gtttgcgccg ggcaaacccc
     5641 gcatcgtcgg gcatgacgcc aagtggtcga gccgcgcctt cgagtatcat gcgaccccgc
     5701 ggcgtttcga cttccccgca gccgatgccc ggttgctcga cctgcttgga accctcggga
     5761 tgcaggtctg ggagtgcttc gcgatgcgcg gctgcgcccg cgtcgacttc cgggtcgacg
     5821 aggacggccg accctggatt ctggagatca acccgaatcc gtgtctctcg cccgatgccg
     5881 gcttcgctgc cgcgctcgat gcggccggga tcccggcgga ggagatggtc gggcgactgg
     5941 tcaggacggc cgctacggac tgagcatgac ctcaccctgc aaagagcatc cccccgggga
     6001 cgcctgggtt catctgcggg agccggcggc acgcgaccgt ggacccgtgg cctcggtgct
     6061 ggtctcgacg ggatttttca gtgatgccga agtcgctgtg gcgttggaag tcttcgatgc
     6121 ggcattgcag ggtaaggact ccggctacga gcatctcgtg gccgaatccg ccggaagggt
     6181 gatcgggtac gtctgctggg gcgggcccat agagatgacg ctctcgagct tcgaccttta
     6241 ctggatcgcc gtggatgcgg cggcccgggg ccgcggggtc ggtcatcggc tgctggccgc
     6301 ggccgagagc ctggcggcgg cgtcgggctg tgcccagctt tttgcggaga cctccggacg
     6361 tgccgattac gcgcccacgc acagcttcta cgaatcctgc ggctacctgc gcgcggccga
     6421 gctcgcggac ttctacgcgc caggtgacgc caaagtcttc tacgtcaaac ggctgaccgg
     6481 cggcaatcag gccccaccgg aatgacggcg ttccgaccgt gttccgagcg cggccctctc
     6541 tagcggggcg cgtctgcgac cagcgcctcc gtcgacatca tcatgccggc cacggacgct
     6601 gcgttgtgca gcgccgactt cacgaccttg caggggtcca gtatgcccat ttccagcatg
     6661 tcgccgaacg tttcggtctg cgcgttgaac ccgaagtgtc cctgctccga ctgaatcttt
     6721 ctcagcacca cgcctggctg cagaccggcg ttggcgacga tctggcgcag gggcgcctcg
     6781 gcagcccgcc gaagcgcgtc cacgccgctt gcctggtcct ggtttcgggg cgcggccttt
     6841 gcgagctcgg ctgaggcgcg cagcagtgcc acgccgcctc ccggcacgac gccctcttcc
     6901 acggcagcgc gagtagcagc gagcgcgtcg tccacgcgca tcttcctctc cttcatggcg
     6961 acttccgtgg cgccgccgac gttgatcacc gccacgccgc ctgccagttt ggcaagacgc
     7021 tcctgcagct tttcgcggtc gtactcggac ttggactcct cgatctcgcg acggatctgc
     7081 gagatgcgcc cggcgatagc gtcggtcccg ccgcgacccc cgatgagcgt cgtgtcccct
     7141 ttggtcagca atacccgttg cgcctcgccg agatcctcca gcgccacctt ttccaggctg
     7201 aatcccgcgt cgctggagat gacctttccg ccggtgagga tcgccaggtc ctgcagcatg
     7261 gccttgcgcc ggtcaccgaa gcccggcgct ttgactgcgg caaccttcag gcggccgcgc
     7321 agcctgttga cgaccagggt cgccagcgcg tcagcctcga cggtctcggc gacgatgagc
     7381 agcgagcggt tggcccgggc gaccgcctcc agcagtggca gcagttcgcg ggagctggag
     7441 atcttcttgt cccgcagcag gatcaccggc cgatcgagct cggcagcata ggtctcggtg
     7501 ctggtgacga agtagggtga gaggtaaccc tgatcgaact gcatgccctc agatacttcg
     7561 agctggtcgt cgatgccggt gccgtccttg acggtaatca caccctcgcg acccacctgc
     7621 tccatggcct gcgcgatgag ctcgccgatg gcctcgtcgc cgttggcaga aatcgtggcc
     7681 accttgcgta tgtcgtcccc ggcctcgcac tcgacggcca tcgcgtccag ggcatcggtc
     7741 atcaggccca cgcccaggtc gatgccgcgc ttgatttcaa cggggctgaa tcctgccgca
     7801 atggcgcgac ggccccagtg aaagagagag tgagcgatca ccgtggccgt tgtggtgccg
     7861 tccccggcct cgtccgcggt ccgcgttgcc gcctggcgga cgattcgcgc gcccatgtcc
     7921 tcgaatcggt ccttcagctc gatgtccttg gcgacggtga cgccatcctt ggtcaccgtc
     7981 ggtgcgcccc aggtcttgct cagcagcacg ttacgtcccc gaggtcccaa ggtggcgttg
     8041 acggcccggg ccaaggtgtc gacgccccgg ttgagccggg ctcttgcgtc atgggcaaaa
     8101 acgatctgct tggcgctcat gcggttttcc tcgggtgccg gttgcggccg gcaaccgagc
     8161 gcccgcactt cgccgccgcc tgtcggttgc gcagttcctg tgctatgagg agggactcct
     8221 cacgggtaat catcgatcgg atccggccgt gcaaaaaatc ttcagatcag actcgcggcc
     8281 agtcgaaaaa gtcacagcag gcgatgaaaa tcagcccgga atgtggtcct tgagcttatc
     8341 cagggtgcgg tagtagcgca tcttcgtcgc gctcaagctg agtcctagca cggccgcgat
     8401 ctcgtgcagc tccatgtcct ccacaaagcg cagcgcgagc agcgcgcgct cttgcggaga
     8461 aagcacggcc agcaggcgct gaaagtcgct ctggtcggga gcgtcttcct cccgctcgtg
     8521 ctgcgccatg cccagcgctt cccgcttgtc ctgttcccgt atctgctgtc ggacccgggt
     8581 gcgggccagg ttctccacga tcctgaacag ccaggtggaa aacttcgcgt cgcctcggaa
     8641 gttcttcagg ccggagaaca cccgcagcat gacgtcctgg gcaagggcat cggcctcgtc
     8701 gggagcgccg agctgcgccg tggccagcct gcgcaccgat tcgtagtgcc gcctgaccag
     8761 ttcgtcatag gccgaagtgt cggcgggcaa ttggtgacgg gcactcgccg ccagtgcctc
     8821 gtcggagagg tcggcaaggg aaacgcggcg ctgtgactcg tggctcacgc gcgcagtcta
     8881 atgtctatgg agccgtggac aaataggcgc ccgtgaaaga tcgttcgaaa ctcgaagccg
     8941 gcctgagaac gctttacgag caggccatgc cggaatctca tgggttcgcg gcgcgcgcgc
     9001 tcagagtgcg cctcgggcga ttgctgggcg ctgccgccaa gaggtccgag caatgccgac
     9061 gacaaccggc aggcggaccg ccgcgagccg gcgatgacga tgccgacgat ggggggtaag
     9121 cggcccgaga accgtgtttc cctgcagctc ctgcagcgga ttctcgtcga tgccagctcg
     9181 gcgcacaccg tggaggagca ggtcgagcgg ctggtttcgc aggtccgtga cgccatggca
     9241 gcgggcgcct gcagcctgta tcagcgcttt gacgacactc tcaggatggt agccaactgc
     9301 gggttcgctc ccgagtccgt cgggcgtatc gcgctgccga tgggtgaggg gctcgttggc
     9361 aggatcgccc gggaccggat gtcgctcaac ctggccgatg cttcggccca tcccgacttt
     9421 cgtctgttcc cgcagtccgg tgaagaggcg tttcccgcct tcctcggggt gccgatcatc
     9481 catctgggcg ccgtcaacgg cgtgctcatc gtgcaggacg atcggccctc ggcgttcagc
     9541 gaggacgagg tctctttcct
//
LOCUS       DBNHOX010000174         3431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_464305, whole genome shotgun sequence.
ACCESSION   DBNHOX010000174 DBNHOX010000000
VERSION     DBNHOX010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_464305"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1001
                     /gene="bamJ"
                     /locus_tag="AC16UZ_03740"
     CDS             324..1001
                     /gene="bamJ"
                     /locus_tag="AC16UZ_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BamJ family beta-barrel-assembly machinery
                     protein"
                     /protein_id="HIR6245826.1"
                     /translation="MGCQPAGDGTAADAQAAEESAELALDDQVAQASYGIGYTIASNL
                     DSQFAGSLNTEALMAGLQDSMEGRERRLSEAAIQTAIAALAQKQQEAVAARSADVLAA
                     GRAFLEENGRRDGVSTTASGLQYEIIATGDGPKPGASDTVSTHYHGTLIDGSVFDSSV
                     ERGQPASFAVNRVIPGWTEALQLMSVGSKWRLFIPPELAYGDRAAGSIPPNSTLIFEV
                     ELLAVDG"
     gene            1049..1318
                     /locus_tag="AC16UZ_03745"
     CDS             1049..1318
                     /locus_tag="AC16UZ_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245827.1"
                     /translation="MSGERRRPELGNTLTKRRMEPRRRGRLARTILLGTVAVAFSLFW
                     VARELELDTEELLGFLGASAVFVGLMVILGLVGAAAVLIFKRLGR"
     gene            complement(1334..2017)
                     /gene="lepB"
                     /locus_tag="AC16UZ_03750"
     CDS             complement(1334..2017)
                     /gene="lepB"
                     /locus_tag="AC16UZ_03750"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR6245828.1"
                     /translation="MTGRLHEHWVTWRGFALFLAIMLVFRSAVADWNQVPTASMFPSI
                     FVGDRIVIDKLAYGLRIPFTHQSITSWDDPHRGDVITFTSPEDERLLIKRVVGIPGDT
                     VALDGNRLVVNGQTARYTELTATERRALDGVADREYRLLRESLLGSQRLVMVRSSRVQ
                     DDDLPPTVVPPGQYLVLGDNRDNSRDSRSIGLVARDRILGKATAVAFSLNYDRYYAPR
                     VDRFFADLP"
     gene            complement(2026..2322)
                     /locus_tag="AC16UZ_03755"
     CDS             complement(2026..2322)
                     /locus_tag="AC16UZ_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HIR6245829.1"
                     /translation="MDKDQENAIEAAVFRRLLEHLDEHKDVQNIDLMNLAGFCRNCLS
                     KWYRAACEDAGVAMDYEAARERVYGMPYADWKSRYQAEATPEQLAGFESGRQQG"
     gene            2439..3218
                     /locus_tag="AC16UZ_03760"
     CDS             2439..3218
                     /locus_tag="AC16UZ_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR6245830.1"
                     /translation="MELLTNPEIWAAFVTLTILEIVLGIDNIIFISILTSRLPPEQRA
                     RGRVLGLGLAMLMRILLLLSLAGIMRLTTDLFEVMGQGISGRDLILIFGGAFLMAKAT
                     REVHNALEGEAHEGQGAGAPTFAGVLLQIALIDMVFSLDSVITAVGLVDEVPVMVAAV
                     ISAVLVMMVAAGPISDFVEDHPTVKMLALAFLLLIGLTLMGEGIEIHTPKGYIYFAMA
                     FSFGVEILNIRAKKRRKDHVMLRKTQLADLVASRSEKGRPD"
     gene            complement(3231..>3431)
                     /gene="yaaA"
                     /locus_tag="AC16UZ_03765"
     CDS             complement(3231..>3431)
                     /gene="yaaA"
                     /locus_tag="AC16UZ_03765"
                     /inference="COORDINATES: protein motif:HMM:NF015820.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HIR6245831.1"
                     /translation="FKDLKNGRYRFMSFFAKRARGMMAGYLVKNRVNTLKGLREFDWG
                     GYYYSEDLSRGDDWVFLRDKPA"
BASE COUNT          589 a         1061 c         1129 g          652 t
ORIGIN      
        1 tggccttcgt attgttcgta actcagtcgc cggggatctg cagattttcg ccggtccggt
       61 cggcaaactc gcttctgcca cccgtttcgg tacaaactgt gccgcaactg atcgctactt
      121 ctgtagatac catttctcct gggtggttcc cccaaaccac tccagcgaga ctatctaaat
      181 gcccgcatgc gtactgttgc gggaatgtaa cccggtgtct gggtgtgggc aaccggggca
      241 gggagcccaa attgatggaa tgccgaacgg acatgagtac ttggtcaaga aatttcgcat
      301 ctttgctggt ggtgctgctg gcgatggggt gccagccggc cggggacggc accgcggcgg
      361 atgcgcaggc ggcagaggaa tccgcagagc tggcgctgga cgatcaggtg gcccaggcca
      421 gctacgggat cgggtacacc atcgcgagca atctggactc ccagtttgcg ggctccctga
      481 acaccgaggc cctgatggcg ggccttcagg acagcatgga gggtcgggag cgccggcttt
      541 ccgaggcggc gatccagacc gccatcgcgg cgctggcaca gaagcagcag gaggcggtgg
      601 ccgctcgctc cgcggatgtg ctggccgccg gccgggcgtt tctggaggaa aacgggcgcc
      661 gggacggcgt cagcacgacg gcgtcgggac tgcagtacga gatcatcgcg accggcgacg
      721 gtcccaagcc gggcgcctcg gacaccgtga gcacgcacta tcatggcacc ctgatcgacg
      781 gcagcgtgtt cgacagctcc gtggaacgcg gccagccggc aagctttgcc gtcaaccggg
      841 tgattccggg ctggacggaa gcgctccagc tgatgtcggt gggttccaag tggcgtctgt
      901 tcattccgcc cgagctcgca tacggagatc gggcagcggg ctccatccct cccaactcga
      961 cgctgatctt cgaggtcgag ctcctggccg ttgacggtta gcacgcacgt ggctcatcgc
     1021 gagcgggtct ggacggcggc cgcttcccat gagcggtgag cgccgccgcc ctgagctcgg
     1081 caacacgctg accaagcggc gcatggagcc ccgcaggcgc gggcgccttg cccggaccat
     1141 actgcttggc acggtggcgg tggcgttctc gctgttctgg gtcgctcggg aactggagct
     1201 cgacaccgag gagctgctgg gtttcctggg tgccagcgcc gtgttcgtcg ggttgatggt
     1261 gatactcggg ctggtgggcg cggccgccgt cctgatcttc aagcgcctcg gccgctgacg
     1321 gcccgctcac ggatcaggga agatcggcaa aaaagcggtc aaccctcggc gcgtagtacc
     1381 gatcgtagtt cagggagaag gcgacggccg ttgccttgcc gagaatccga tcccgggcca
     1441 ccagaccgat gctgcgcgag tcccggctgt tgtctcgatt gtcgccgagc accaggtatt
     1501 ggccgggagg cacgacggtg ggcggcaggt cgtcgtcttg cacacgggag cttcgcacca
     1561 tcaccagtcg ctggctgccc agcagagatt cccgcagcag ccgatactcc cggtcggcga
     1621 cgccgtccag cgcccggcgt tcggtggccg tcagctcggt gtagcgggcg gtctggccgt
     1681 tgacgacgag ccggttcccg tcgagcgcga cggtgtcgcc gggtatcccg acaacgcgct
     1741 tgatcagcag ccgctcgtct tcgggcgagg tgaaggtgat cacgtcaccg cgatgggggt
     1801 catcccagct ggtgatggac tggtgcgtaa aaggaattct cagcccgtag gccagcttgt
     1861 cgatcacgat gcgatcgccg acgaagatgg agggaaacat ggaagccgtt ggcacctgat
     1921 tccagtctgc cacggcggag cgaaagacca gcatgatggc cagaaagagg gcaaagcctc
     1981 gccaggtgac ccagtgctcg tgcagtcggc cggtcacgct cctgatcagc cctgctgccg
     2041 gcccgattcg aagccggcca gctgctcggg cgtcgcctcg gcctggtagc gggacttcca
     2101 atccgcgtag ggcataccgt agacccgctc ccgcgccgct tcgtagtcca tggcgacgcc
     2161 ggcgtcctcg caggcggccc gataccactt cgacaggcag ttgcggcaga aaccggccag
     2221 gttcatcagg tcgatgttct gcacgtcctt gtgctcgtcg agatgctcca gcaggcgccg
     2281 aaacaccgcg gcctctatgg cgttttcctg atctttgtcc acgacggatc ccaaagttga
     2341 cacgcgttgg ccgcattcta ccgaaaactt gcggcctggg tgcttcggca gaaatcctgt
     2401 gcttcaatgc ccgcaagtca accggctgac ggcagattgt ggagcttctc accaacccgg
     2461 agatttgggc cgcgttcgtc acgctgacga tcctcgagat cgtgctcggg atcgacaaca
     2521 tcatcttcat ctccatactc accagtcgcc tgccgcctga gcagcgtgcc cgcggccgcg
     2581 tgctcggtct tggcctggcc atgcttatgc gcattctgct gctgctctcg cttgccggga
     2641 tcatgcggtt gaccacggac cttttcgagg tgatgggcca gggcatatcg ggccgcgacc
     2701 tgatactgat cttcggcggc gccttcctca tggcgaaagc cacccgggaa gttcacaacg
     2761 ccctggaagg tgaagctcac gaaggtcagg gcgccggcgc gccgacgttc gcgggcgttc
     2821 tgttgcagat cgccctgatc gacatggtct tctcgctgga ttcggtgatc acggcggtag
     2881 gcctggtgga cgaggtgccg gtgatggttg ccgccgtgat ctccgcggtg ctggtgatga
     2941 tggtcgccgc gggaccgatc agtgatttcg tggaagatca cccaacggtg aagatgctcg
     3001 cgctggcgtt tctgcttctc atcgggctca cgctgatggg cgaaggcatc gagatccaca
     3061 cgcccaaggg atacatctac tttgccatgg cgttttcgtt cggagtggag atcctcaaca
     3121 tccgggccaa gaaacgcagg aaggatcacg tgatgctgcg caagacgcag cttgccgatc
     3181 tggttgcctc gcggtcggag aagggccggc cggattagcc ggccgccggc tcaggcgggc
     3241 ttgtcacgca gaaagaccca atcgtctccc cgggacagat cctcggaata gtagtagccg
     3301 ccccagtcga actcgcgaag ccctttgagc gtgttgaccc tgttcttcac caggtagccg
     3361 gccatcattc cccgcgcccg ctttgcgaag aaggacatga agcggtacct gccgttcttg
     3421 aggtccttga a
//
LOCUS       DBNHOX010000175         5056 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_335485, whole genome shotgun sequence.
ACCESSION   DBNHOX010000175 DBNHOX010000000
VERSION     DBNHOX010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_335485"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="AC16UZ_03770"
     CDS             <1..1594
                     /locus_tag="AC16UZ_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6245832.1"
                     /translation="DFTDLSQLQGVVLAPPPPMAFIHNVTAAAAGPGQPCAVTDVNGN
                     GITYNPSIPNCYDSRYIDPESGENQGTAPAFSKTDLFGVSANLRWDLSDTLTLKSITS
                     WRDLDSEFARDGDHSPQRISQFFDDLEQQQFTQEFQLLGAHERLNWILGAYYFTEDGD
                     NVNILDFTVSNFRSGGEFDNEAWAVFAQATFDITDRLHLTAGGRYTEEEKSFLPDQII
                     FQNYYAGISELVPEGNPLAALDAPFLQAGERILPYLEKRIDIDEFTPMVNVSFDATDD
                     VMLYATYSEGFKSGGFTQRVFPPIVPGFTAPPGTPDIDLIPTYEPEFVKSYEIGAKAT
                     GWDGRLRLNGAVFRSDYKDLQVQVFNSVAPVTQNIGKASINGLELEVTLVPADDWLIE
                     GSLSWLDAEYDEIDTGITLIGEDNEFERVPEFTASAGISKEFQLDDAGSLVVRADWSY
                     RSETFNDAYNTPQLETDGYHLIDASVRWRNAGEDWTVALSGRNLTDEKYLVSGVYGTA
                     FQSYEGMFDRGSQWRLEVRKEF"
     gene            1628..2206
                     /locus_tag="AC16UZ_03775"
     CDS             1628..2206
                     /locus_tag="AC16UZ_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6245833.1"
                     /translation="MARVKLAKPESVDDPAVAGIFEWVTQMEGSVPNHFYVEMNFPEF
                     FTAKLGATKVLWEAGELTMPEIQHIGILVSKDNGCRYCTAAFCTILNHGLEAAEDYVA
                     ELVSVGTDAVSDLRLRTMLEYALQVNRDAAGVTDGDVARLRNIGLSDKGIVQLTHIVS
                     DFASYNRLNLALQTDYDYRDLWRQLAFGDGAG"
     gene            complement(2330..3586)
                     /locus_tag="AC16UZ_03780"
     CDS             complement(2330..3586)
                     /locus_tag="AC16UZ_03780"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6245834.1"
                     /translation="MKLKTGADVDATPLEEIDVSRPELYLNDTWQPWFARLRREAPVH
                     FLADSVNGPFWSVSSHQLIKEVDRNHQVFSSEKGGIAIVDPYVMEEGQVQGRSFIALD
                     EPKHMPQRMAVAPTVAPKNLAELEPLIRERAIDILDHLPVGETFNWVQEVSIELTARM
                     LATLFGFPYEDRRKLVYWSDLATAVPEVTGDDSIDLSKRYEELMGAAAAFYQLWAERA
                     QEPPKFDLISMLIHNEQTAKMNEDPEQFLGNILLLIVGGNDTTRNSISGGVIALNRYP
                     GEYQKLRDNPGLIPNMVSEIVRWQTPVIHMRRTALQDCELGGKQIREGDKVVMWYLSG
                     NRDETVFDDPDRLIIDRPNARAHVAFGFGVHRCMGNRLAELQLRILWEEIMKRFHTVE
                     VVGDVERLSNNFIRGIKDVPVRLHPT"
     gene            complement(3691..4128)
                     /locus_tag="AC16UZ_03785"
     CDS             complement(3691..4128)
                     /locus_tag="AC16UZ_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HIR6245835.1"
                     /translation="MSDEPSYGTGDATFRAAGGEAGIRRLVDAFYDIMERDPRYRRIW
                     DWHPPDNEVSRDKLARFLCAWMGGPRLFSEKYGRISIPSAHAHLGVTAVERDLWLDCM
                     ARALAQQDYPESLVAYLLKELAVPAERIRQVTEGGAKSETGDR"
     gene            complement(4216..>5056)
                     /locus_tag="AC16UZ_03790"
     CDS             complement(4216..>5056)
                     /locus_tag="AC16UZ_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6245836.1"
                     /translation="DILDGLPVGEPFNWVERVSIELTTQMLATLFDFPFEERYKLTRW
                     SDIAISPRKAGLWDTLEERERELDECLSRMTELRDERIRQPGGHDLLSMLAHSESTRN
                     MPPRQFLGNLLLLIVGGNDTTRNSISGGVLAMNRWPEEFEKLKADRSKIPNAVAEIIR
                     WQTPLAFMRRTTTEDVEIGGQTIPAGERVLMWYASGNRDENVFENADLFEVDRGNARQ
                     HLSFGFGVHRCMGNRVAELQLKILWEELLERYSRIEVAGAPVRAISNFVRGYTHLPVK
                     LHT"
BASE COUNT          973 a         1614 c         1524 g          945 t
ORIGIN      
        1 cgacttcacc gatctcagcc agctgcaggg tgtggttctg gcaccgcctc cgcccatggc
       61 attcattcac aacgtcaccg ccgccgccgc aggacccggc cagccctgcg ccgtcacgga
      121 cgtcaacggc aacgggatca cctacaaccc gagcatcccg aactgctacg acagccgcta
      181 catcgatccg gaaagcggcg aaaatcaggg caccgcgccc gcgttctcca agacggacct
      241 cttcggcgtg tccgcaaacc ttcgctggga cctctccgat acgctgacgc tgaagtccat
      301 cacgagctgg cgggatctcg actccgagtt cgcccgggac ggggatcact cgccccagcg
      361 catctcgcag ttcttcgacg atctcgagca gcagcagttc acccaggaat tccagctcct
      421 tggtgctcac gaacggctga actggatcct gggcgcctac tacttcaccg aggacggcga
      481 caacgtgaac atcctggatt tcaccgtctc caacttccgc tccggcggcg agttcgacaa
      541 cgaagcgtgg gccgtcttcg cccaggcgac cttcgacatc accgaccggc tgcacctgac
      601 cgcgggcggc cgctacaccg aggaagagaa gtccttcctg ccggatcaga tcatcttcca
      661 gaactactat gcgggcatca gcgagctggt acccgagggc aaccccctgg ccgcgctgga
      721 tgcgcccttc ctccaggcgg gcgagcggat cctgccgtac ctcgagaaac gcatcgacat
      781 cgacgagttc acccccatgg tgaacgtctc gttcgatgcg accgacgacg tcatgctcta
      841 cgccacttac tccgaagggt tcaagtcggg gggcttcacc cagcgcgtct tcccgcccat
      901 cgttcccgga tttaccgcgc cgccggggac gcccgacatc gacctgatcc ccacgtacga
      961 acctgaattc gtgaagtcct acgagatcgg cgccaaagcc acgggttggg acggccggct
     1021 gcgtctgaac ggcgccgtgt tccgaagcga ctacaaggat ctgcaggtac aggtgttcaa
     1081 cagcgtcgcg cccgtcaccc agaacatcgg caaggcgtcc atcaatggcc tggagctgga
     1141 ggtgaccctg gtgccggcgg acgactggct gatagaaggc agcctgtcgt ggctggacgc
     1201 cgagtacgac gagatcgaca ccggcatcac gctgatcggc gaggacaacg agttcgagcg
     1261 ggtgccggag ttcacggcca gcgccggcat ctccaaggag ttccagctcg acgatgcggg
     1321 ctcgctggta gtgcgcgccg actggagcta ccgctcggaa accttcaacg atgcctacaa
     1381 cacgccgcag ctggagacgg acggctacca cctgatcgac gccagcgtcc gctggcgcaa
     1441 cgcgggcgag gattggaccg ttgccctgtc aggccgcaac ctgaccgacg agaagtatct
     1501 cgtcagcggc gtctacggca cggccttcca gtcctacgaa gggatgttcg atcgtggcag
     1561 ccagtggcgg ctggaagtcc gcaaggagtt ctagcccgat tcacagggga cgacgagagg
     1621 ccgaaacatg gcaagagtga aactcgccaa gcctgagtcc gttgacgatc cggcagtagc
     1681 cggcatcttc gagtgggtga cccagatgga gggcagcgtg cccaaccatt tctacgtgga
     1741 gatgaatttc ccggagttct tcacggcgaa gctcggcgcc acaaaggtgc tctgggaggc
     1801 cggtgagctg accatgcccg agatccagca catcggcatc ctggtctcaa aagacaacgg
     1861 ctgccgctac tgcacggcgg cattctgcac catcctcaac cacggcctgg aagcggcgga
     1921 ggactatgtg gccgaactgg tgagcgtcgg aacggacgcc gtttcggacc tgcggctgag
     1981 gacgatgctg gagtacgcac tgcaggttaa tcgggatgcg gccggcgtca ccgacggcga
     2041 cgtcgcccgc cttcggaaca tcgggctgag cgacaagggc atcgtccagc tgacgcacat
     2101 cgtcagcgac ttcgcttcct acaaccgtct gaacctggcg ttgcagaccg actacgacta
     2161 tcgggacctc tggcgccagc tggcttttgg cgacggcgcc ggttagcttc ctgctgctgc
     2221 tgtggcgatg atctgcgtgg atgcgatcgc caaggtttcc gcgctcacga cggagggtgc
     2281 cttagggctg gaggaaggca acgtcgagag cgccgcctga acgacgagct caagttgggt
     2341 ggagccttac ggggacgtcc ttgattcctc tgatgaagtt gttggacaaa cgctcgacgt
     2401 cgcccaccac ttcaacggtg tgaaagcgct tcatgatctc ttcccagaga atgcgcagct
     2461 gaagctccgc cagccggtta cccatgcagc ggtgcacccc gaagccgaac gcgacgtggg
     2521 ccctggcgtt cgggcgatcg atgatcagcc ggtccggatc atcgaaaaca gtctcgtctc
     2581 gattaccgga gaggtaccac atcaccacct tgtcgccctc cctgatttgc ttgccaccga
     2641 gctcgcaatc ctgcagggcc gttcggcgca tgtgaatgac cggtgtctgc caacgaacta
     2701 tttcagaaac catgtttggg atcaggccgg gattgtcccg cagtttctga tactcacccg
     2761 gatagcggtt cagagcgatc acgcctccgc tgatgctgtt ccgggtggtg tcgtttccgc
     2821 ccacgatcaa cagcagtatg ttgccgagaa actgctcagg gtcctcgttc atcttcgcgg
     2881 tctgctcgtt gtggatgagc atggagatca gatcgaattt tggcggttcc tgggcccgtt
     2941 ccgcccacaa ctgatagaac gccgccgccg cgcccatcag ctcctcgtag cgcttgctca
     3001 gatcgatgct gtcgtctccg gtcacctcgg gcaccgcggt agccaggtcc gaccagtaca
     3061 ctagcttgcg gcggtcctcg tacgggaaac caaacagcgt ggccagcatc cgggccgtca
     3121 gctcgatgga tacttcctgc acccagttga aggtttcgcc aaccggcagg tggtcgagga
     3181 tgtcgatggc tctctcacga atcagcggct ccagctccgc caggttcttc ggagcgaccg
     3241 tgggagcgac ggccattctc tgcggcatgt gtttcggctc gtccagcgcg atgaagctgc
     3301 ggccctgtac ctggccttcc tccatcacgt aagggtcgac gattgcgatc ccgcctttct
     3361 ccgacgagaa cacctggtgg tttctgtcga cctccttgat cagttgatga ctggagaccg
     3421 accagaaagg gccgttcacg ctgtccgcaa gaaagtgcac gggcgcttcc cttctgaggc
     3481 gggcgaacca cggctgccag gtgtcgttca ggtaaagttc cggacgactg acgtcgatct
     3541 cctcgagggg ggtggcatcg acatccgcac ctgtcttcaa cttcatccga gcctcctgat
     3601 caagccgtaa agtgaacgat ttcagaagca tatcaccgac cggaaatccg atcgcgaccg
     3661 ggcgcgcttc gaggctactg ctgagagctg ctaacggtct ccagtttcag attttgctcc
     3721 gccctcggtt acctgtcgaa tacgctcggc gggtacggcg agctccttga ggaggtatgc
     3781 gaccagcgac tcggggtaat cctgctgcgc aagcgctcgg gccatgcagt cgagccagag
     3841 atcgcgctcg acggcggtga cgccaaggtg cgcgtgggcg gacggaatgc tgatgcgacc
     3901 gtacttctcg ctgaacaacc gcggaccgcc catccaggcg cagagaaagc gcgcgagctt
     3961 gtcgcgtgat acttcgttgt ccggcgggtg ccagtcccag atacgccggt atctggggtc
     4021 ccgctccatg atgtcgtaaa aagcgtctac caaccgtcgg atgccggctt ccccaccggc
     4081 ggctctgaag gtggcatctc ccgtgccgta gctgggctcg tcgctcacga ccgctgcgcc
     4141 ccaggagaga cgtgttcgcc gccggtaacg tcgtgaaagg tcacctcgag aggcccattg
     4201 cccgatgacc cacggtcagg tgtgcaactt cacgggcagg tgagtgtagc cgcgaacgaa
     4261 gttggagatg gccctcaccg gagcgcctgc cacttcaata cggctgtaac gctccagcag
     4321 ctcctcccag aggatcttga gctgcagctc ggcgacgcga ttgcccatgc agcggtgcac
     4381 accgaagccg aaggagaggt gctggcgagc gttcccgcga tcgacctcaa agaggtcggc
     4441 attctcgaaa acgttctcgt cccgattccc ggacgcgtac cacatcaata ccctttctcc
     4501 cgcgggaatg gtctggccac caatctcaac atcctccgtc gtcgtgcgac gcatgaacgc
     4561 cagcggcgtc tgccagcgga tgatctcggc cacggcgttc ggaatcttcg agcggtcggc
     4621 tttgagcttc tcgaactctt ccggccaccg attcatggca agcacaccgc ccgagatgga
     4681 atttctcgtg gtgtcgttgc caccaacgat gagcagaagc aggttgccca ggaactggcg
     4741 cggcggcatg ttgcgggtac tttcgctgtg cgcaagcatc gagagcaggt cgtgaccgcc
     4801 cggttggcgg atccgctcgt cgcgcagctc ggtcatgcgc gacaggcact cgtcgagctc
     4861 ccgctcgcgc tcttccagcg tgtcccagag gcccgctttg cgtggagaaa tcgcgatgtc
     4921 cgaccagcgc gtcagcttgt agcgttcctc gaaagggaaa tcgaacaggg tcgcgagcat
     4981 ctgcgtggta agctcgatgg atacgcgctc cacccagttg aagggctcac ccacaggaag
     5041 accgtcgaga atgtca
//
LOCUS       DBNHOX010000176         3612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_206737, whole genome shotgun sequence.
ACCESSION   DBNHOX010000176 DBNHOX010000000
VERSION     DBNHOX010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_206737"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1627
                     /locus_tag="AC16UZ_03795"
     CDS             92..1627
                     /locus_tag="AC16UZ_03795"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6245837.1"
                     /translation="MVETVTGTNRSAGVSYSEVLDRDSHPVRDILRVDNPLPPGPTIV
                     DPKVYYSREVFEREKERLWKRVWQMACHEDDIPNVGDAIVYDIADLSYMVVRSAEDEF
                     HAFPNACLHRGRQILHQNEERLHNLRCAFHGWSWGLDGELQEVPCHWDFPTVSKETHS
                     LKQVKVGRWGGFVFINPDPDCEPLEDFLGNLDEHMTLLPMERRYKAVHVAKRLPCNWK
                     VAHEAFAEAYHSVATHPTLLSTLGCANSKYDIFGNFSRALSAHHVGSPHVPSWLAEHP
                     YLEATPFTKQRHELTGSIYERVDDGRVKVTTTDNRVGYFDDRGNHIDGEKFHADIHLC
                     NWVGGKLIEGMDELPSSVITGDFAEKRAKAAAVRREELRKILGDKMDEVSDAELLDPI
                     YYSLFPNVSPWGSFNQIFYRFRPYGDNPEECIHECLFMWPVAEGQPRPPAAKMQWLDL
                     DDDYVDAPELGHLAKVFNQDTLNLPYVQKGLHSLDEVIFANYGETKPRHFHMLYNEWM
                     SKE"
     gene            1743..>3612
                     /locus_tag="AC16UZ_03800"
     CDS             1743..>3612
                     /locus_tag="AC16UZ_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR6245838.1"
                     /translation="MKLLSPVEVGPIEVKNRVVSTAHAAFLEFFNPVSDGERYMAYQE
                     RRAQGGTGLIIFTAMHVHESSQIPYHYVFDADTMAPKFAQISTRLHRHGAKCISQLFH
                     FGAQGKSDPRDDFHPLWSFSGTTTLEGEVTHKMTDAEIEEVIDAFAFAAKVATENGMD
                     GVELHGTHGYLIQQSFSPFANQRDDKWGEELVFVKTLARRVRDAIGPGKIMGFRISAD
                     DFIDPADGGVGHERLCHIASEVVGTGLFDYLNHSEGAGGVHYARAIGSFRHKFGEYLP
                     LTRNLRDAIGGAVPLIGVGKIPTPDLAEQALQAGDCDLVGMTRAQIADPDLVSKVAAG
                     RAHRVRTCTGSNQGCFERASYPITCFQNPEVGEENRFKALDIPVVQLKKVLVVGGGPA
                     GMKAAEIAARRGHSVTLAESGDRLGGRLNLVETAGDATNLLSATAWIEEELGGLDVDI
                     RLQTTVDEAFVRQVNPDAIVLATGAYVTNDLGVATDGSVQVLSTDDAASGTFEGNRFE
                     LEGTRCLMIDFRGNYETAVVLESLGRRGSKVTLVTPAPVFGRNIGVSHADDFRRTVIP
                     QLGIRLLPNTTLAQISQGRGHLVNHVTGEEIEEFFDFIVAGTPPRPRDVLFEVLGRH"
BASE COUNT          691 a         1125 c         1138 g          658 t
ORIGIN      
        1 ttggccgtac gcagtttccg ctattgcccc ccggtctgat agatgcgctg atagatgagg
       61 cgcatttgat ctgctcggag ggggcgaatt catggtcgaa acagtaacgg gcacgaaccg
      121 atccgcaggc gtcagttaca gcgaggtgct cgaccgcgac tctcacccgg tgcgggacat
      181 cctgcgtgta gacaacccgt tgccgccggg cccgacgatc gtcgacccga aagtctatta
      241 ctcccgtgag gttttcgaac gggagaaaga gcgcttgtgg aagcgggtct ggcagatggc
      301 gtgccacgag gatgatattc ccaacgtcgg cgacgccatc gtctacgaca ttgcggacct
      361 ttcctacatg gtcgtgcgca gcgcagagga cgagttccat gcgttcccga atgcctgcct
      421 gcaccgcggc cggcagatcc tgcaccagaa cgaagagcgt ctgcacaatc ttcgctgcgc
      481 tttccacggc tggtcctggg ggctcgacgg cgagctgcag gaggtgccct gtcactggga
      541 tttccccacc gtctcgaagg agacccactc cctgaagcag gtgaaggtcg gccgctgggg
      601 cgggttcgtg ttcatcaatc ctgatccgga ttgcgaaccg ctggaggact tcctcggcaa
      661 tctggacgag cacatgacgc tgctgcccat ggaaagacgt tacaaagccg tgcacgtcgc
      721 caagcggctg ccgtgcaact ggaaggtcgc ccacgaggcg ttcgccgaag cctatcactc
      781 ggtagcgacc cacccgacgc tgctcagcac gctggggtgc gccaactcaa agtacgacat
      841 cttcgggaac ttttcccgcg ccctgtcggc ccatcatgtt ggcagccccc acgtgccatc
      901 gtggctggcg gagcacccct atctggaggc gacgccgttc acgaagcagc gtcacgagct
      961 caccggcagc atttacgaac gagtcgatga cgggcgcgtc aaggtcacca ccaccgacaa
     1021 ccgcgtgggc tacttcgatg atcgtggcaa ccacatcgat ggtgagaagt tccacgccga
     1081 cattcacttg tgcaactggg tgggcgggaa gctgatcgag gggatggacg agctgccctc
     1141 ttcggtcatc acgggcgact ttgccgaaaa gcgtgcgaag gcggctgccg tgcggcgcga
     1201 agagctgcgc aagattctcg gcgacaagat ggacgaggtg agcgacgcgg agctgctcga
     1261 tcccatctac tacagcctgt tccccaacgt cagcccctgg gggtctttca accagatctt
     1321 ctaccgtttc cgaccttacg gcgacaaccc ggaggagtgc atccacgagt gtctgttcat
     1381 gtggccggta gccgaaggac aaccgcgtcc gccggcggcg aagatgcagt ggctggatct
     1441 ggacgatgac tatgtcgacg cgccggagct cggacatctc gcgaaagtct tcaaccagga
     1501 caccctgaac ctgccctacg tgcagaaggg gctgcattcg ctggacgagg tgatcttcgc
     1561 caattacgga gaaaccaaac cgcgccactt ccacatgctc tacaacgagt ggatgtcgaa
     1621 ggagtaagca aggcgggcgg aattcgctga gccgagcggg ggccgatgcc tgtcgaacga
     1681 atcgagacat ttgctctgtg gcggtactac actcgaagta tcggtctgcg aggaacgcca
     1741 tgatgaagct gctcagccct gtcgaggttg gcccgatcga ggtgaagaac cgggtggtct
     1801 ccaccgccca cgctgcgttc ctcgagttct tcaatcccgt ctccgatggc gaacgctaca
     1861 tggcgtatca ggagcgtcgt gcccagggcg ggaccgggct gatcatcttc acggccatgc
     1921 acgtacacga atcgagccag atcccctacc actacgtttt cgatgcggac accatggcgc
     1981 cgaagttcgc gcagatctcg acgcggctgc atcggcacgg ggcgaaatgc atctcccagc
     2041 tgtttcattt cggtgctcag ggcaagtcgg accctcgcga tgactttcat cctttgtgga
     2101 gcttctccgg aaccacgacg ctggaaggcg aggtcaccca caagatgacc gacgcggaga
     2161 tcgaggaggt catcgatgct ttcgccttcg ccgccaaggt ggctaccgag aacggcatgg
     2221 acggcgtgga gctgcacggc acccacggct atctgatcca gcagtctttc agcccctttg
     2281 ccaaccagcg cgacgacaag tggggtgagg agctcgtttt cgtgaagacc ctggcgcgcc
     2341 gggttcggga tgccatcggg cctggaaaga tcatgggatt tcgaatctcg gccgacgact
     2401 tcatcgatcc ggccgacggc ggcgtgggcc atgagcggct atgccacatc gcctccgagg
     2461 tggtgggcac gggcctcttc gactacctga accattccga gggtgccggc ggcgtgcact
     2521 atgcgcgcgc catcggctcg ttccggcaca agttcggcga gtacctgccg ctgacgcgca
     2581 acctgcgcga tgcgattggg ggtgcggtgc ccctcatcgg tgtggggaag attccgaccc
     2641 cggatctggc ggagcaggcg ctccaggccg gcgactgcga tctcgtcggc atgacccgcg
     2701 cgcaaattgc ggatcccgat ctggtcagca aggtggccgc gggccgagcc catcgggtgc
     2761 gcacctgcac cggctccaac cagggctgct tcgagcgcgc ctcgtatcca atcacctgtt
     2821 tccagaaccc cgaggttggt gaggaaaacc gcttcaaagc cctcgacatc cccgtggtcc
     2881 agctcaagaa ggtgctggtg gtgggcggtg gtccggcggg catgaaggcg gctgagatag
     2941 ccgccaggcg cggacactcg gtcaccctgg ccgagtctgg tgaccggctt ggcggacggc
     3001 tcaacctggt ggaaacggcc ggcgacgcca ccaacctgtt gagcgcaacc gcatggatcg
     3061 aagaggagct tggtggcctg gatgtcgata tcaggctgca gaccaccgta gacgaagcat
     3121 tcgtccgcca ggtgaatccg gacgccatcg tcctcgccac cggcgcctac gtgaccaatg
     3181 acctcggcgt cgcaaccgac ggttccgtgc aggtgctgtc caccgatgac gcagcctccg
     3241 gcaccttcga gggcaaccgc ttcgagctgg aagggacgcg ctgtttgatg atcgatttcc
     3301 gcggcaatta cgaaacggcc gtggtgctcg aatcgctcgg acgccggggc agcaaggtta
     3361 ccctggtgac cccggcgccg gtcttcggcc gcaacatcgg cgtctcccac gccgacgact
     3421 tcagacgcac ggtcattccg cagctgggaa tccgcttgct gcccaacacg acgctggcgc
     3481 agatcagcca gggcaggggc catctggtca atcacgtgac gggtgaggag atcgaagagt
     3541 tcttcgactt catcgttgcc ggcacgccgc cccggccccg ggacgtcctc ttcgaggttc
     3601 tcggtcgcca tg
//
LOCUS       DBNHOX010000177         6164 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1492667, whole genome shotgun sequence.
ACCESSION   DBNHOX010000177 DBNHOX010000000
VERSION     DBNHOX010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1492667"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="AC16UZ_03805"
     CDS             complement(<1..392)
                     /locus_tag="AC16UZ_03805"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6245839.1"
                     /translation="MSREQLIEMTRHAIDHAHAGTIDQEPDVMKIPAANYYDPDRFKV
                     EVKQIFRRMPLVLAPTAEIKNPGDYKAMDAAGMPVLLTRGQDGQVRAFINSCSHRGTA
                     LVIDETGNRRKFTCPYHGWTFDQQGTLVG"
     gene            complement(433..1347)
                     /locus_tag="AC16UZ_03810"
     CDS             complement(433..1347)
                     /locus_tag="AC16UZ_03810"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6245840.1"
                     /translation="MSASPLRNVTTEEIESFHRDGAVLVKGVLSDEWVSLIARGLDYA
                     DAHPDGMSEGVEQPLRIDQFPAARSPDLRRAIDASPIAEIVGVTLDSPVRFYMDQMFI
                     KPAGELVPTPWHQDTCYYNVEGHDLIRAWVCVDPVPREASLEVVRGSHLWNVTYRTVA
                     GRSEAEDPERAERAARAYAAGEAFIGPESHERWSYGDAYLDESLPPIPDVEGHRDSFE
                     VLGWDYEPGDVLLFHGNILHGARGGVTLTHPRRSHASMWAGRDVRYIHRRGQVVPDPA
                     ALYALEPRDGQLLGDFPDVFPLAWSPKH"
     gene            complement(1347..1982)
                     /locus_tag="AC16UZ_03815"
     CDS             complement(1347..1982)
                     /locus_tag="AC16UZ_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6245841.1"
                     /translation="MNTVPATAASVALDPLTQTLIDAAEACFVRHGVAKTTMSHVAEE
                     AGVSRTTLYKRFAVMEEVLQAVFIREFDRFEARLAGNLEPIDDPGERLVEIVVAIAEN
                     VPENAGLARLLDGPRTRTENMALAAGRRALNDRVIDMIGDPLDGLARAGVLRGDVERG
                     ELIEWIRRVVNSLAVFPQPRSRSSAERRRFVARFLLPSITAAHDLPPMEVC"
     gene            complement(1979..3709)
                     /locus_tag="AC16UZ_03820"
     CDS             complement(1979..3709)
                     /locus_tag="AC16UZ_03820"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6245842.1"
                     /translation="MHLDLVIRNGMLIDGRRTPSYRADVGIRDGKVAVIGRIAPGSGD
                     RELDADGLIVAPGVVDLHTHYDSQIFWDPWCRISGQHGVTSVVVGNCGFGFAPVRPEH
                     RDRAMLTMARNEAVPLECMQQGMPWDWTTYPEFLDSLDRTPKGVNVLSYVGLNPLMVW
                     LTGSVEEAKGRPLTRAEKAEAERLLAEAMDAGACGWSAQFIGADTVQRDHDGTPMITD
                     LMSREDIEFFAAALGRLGRGGIQMIGPPKMADVLAAASGRPVIYNVLAARSDQHGSPA
                     ESYKDVIAWLARANAEGNRIIAHAVTCAIDYQFTMEDWNLFDSSPLWRELTIGTVPER
                     KAKMMDAQRRAALHEEWAAGKAPIAGGGTEQREERIGVKFQDLVLAYVTEEGEHLRSW
                     EGHTVAEIAAGMGVHPIDAFLDVSIACDLKAGWQSEPRTIDPQVMRDIANCPYAVPGL
                     SDGGAHTKFLTTGTYPTEFLATWVRDNGVMDLEEAHWRLSAYSAQTAGLTDRGFIAEG
                     MPADIVVYDLQALELLPSERVADWPGGAWHLDRRANGWRWTIVNGAVTFADGVCTGAT
                     PGRLLRHGHA"
     gene            3883..4614
                     /locus_tag="AC16UZ_03825"
     CDS             3883..4614
                     /locus_tag="AC16UZ_03825"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6245843.1"
                     /translation="MQEFHVNVATPDGEMECFAAHPDGEGPFAAVILYMDAPGIREEL
                     RDFCRRIAGQGYFCLLPDLYYREGRVRFDLRKGEEELKRMFAVGSRLTVEAVMRDTGG
                     MLAYLEDNPLARSPTGCIGYCMSGQFVVAAAGTFPGEIAAAASLYGTRIVTDKPDSPH
                     KLAERMKGELYLGFAEHDHWVEDFVIPKLIEELDQHGVKYELEVHPGTEHGFCFPERP
                     SYVEAAAEKVWDTVFELFDRRLNAR"
     gene            4637..5287
                     /locus_tag="AC16UZ_03830"
     CDS             4637..5287
                     /locus_tag="AC16UZ_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6245844.1"
                     /translation="MKGLTERQLKRRERILAAARKLITDRGYDGLTMRDLAAESGVAP
                     KTLYHQFHSKENLLRLAVEERYRYLYQIIDDEQIEHGIDRLFFIIDTVASTTENNLAY
                     AKALRPLLTSETSSTFAAIRMNTYRKAIDQIEAEGEVVDWIDVDILAAVVYRQVNPIY
                     LAPWYKETPWATVARIAKLDICLILAAVTTGYTQTTAEKTIRDLQKQLKRSGSRYI"
     gene            complement(5294..>6164)
                     /locus_tag="AC16UZ_03835"
     CDS             complement(5294..>6164)
                     /locus_tag="AC16UZ_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR6245845.1"
                     /translation="KMGMTFVLDRETGQPLFPVEERPVPQEGAAPGEYLAPTQPFPLK
                     PEPLHKLGISPDDAWGFTFLDRGWCRDALEAMTTGPIYTPISVNKETPLYPSVIGGQN
                     WGAPAVDPERQIMVANTHHVAMILNLIPRDQCDGTENFPQEGSPYCATLRPFLSRFGA
                     PCTKPPWGTLSAVNLSTGDIEWQVPLGNLKYQAFWPFTMMKGGVQMGGPTITSAGLIF
                     IAASADRHVRAFDLDTGEELWADEMPTTGNAVPMTYVSGGRQYVVIAAGGHFTSPSPP
                     ADYLIAYALPEKD"
BASE COUNT         1096 a         2090 c         1922 g         1056 t
ORIGIN      
        1 cccaccagcg tgccctgctg atcgaacgtc cagccgtgat agggacaggt gaatttccgg
       61 cggttgcccg tctcgtcgat caccaaggcc gtgccccggt gactgcagct gttgatgaat
      121 gcgcgcacct ggccgtcttg cccgcgggtg agcagaacgg gcatgccggc cgcgtccatg
      181 gccttgtagt cgccgggatt tttgatctcc gccgtgggcg cgagcacgag aggcatgcgg
      241 cggaatatct gcttcacctc gaccttgaag cggtccgggt cgtaataatt ggctgccgga
      301 atcttcatca cgtccggttc ctgatcgatc gtcccggcgt gggcgtgatc gatggcgtgc
      361 cgcgtcatct cgatcaactg ctctctggac atctgcttac ctccctcgct ttcgaaatct
      421 taccctattg aactagtgct tcgggctcca cgccagcgga aacacgtccg gaaaatcgcc
      481 taacagttgc ccgtccctcg gctccagcgc gtagagcgcg gccggatcgg gcaccacctg
      541 ccctcgacgg tgaatatagc gcacatcgcg tcccgcccac atggacgcat gagaacggcg
      601 cgggtgcgtc agcgtcaccc cacctcgggc accgtgcagg atgttgccgt gaaacagcag
      661 gacgtcgccc ggctcgtaat cccagccgag cacctcgaac gagtcccgat gcccctccac
      721 gtcgggaatc ggtggcaggc tctcgtcgag gtacgcatcg ccgtaggacc accgctcgtg
      781 gctctccggc ccgatgaagg cctcccccgc ggcgtaggcg cgggccgcac gctcggcacg
      841 ctcggggtct tcggcctcgc tgcgtccggc cacggtccgg taggtgacgt tccacaggtg
      901 cgatccacgc accacttcca ggcttgcctc ccgcggcacc gggtcgacgc acacccaggc
      961 gcgaatcaga tcgtggccct cgacgttgta gtagcaggta tcctgatgcc acggggtagg
     1021 caccagctcc cccgccggct tgatgaacat ctgatccatg tagaaccgca ccggcgagtc
     1081 cagcgtcacg ccgacgatct cggcaatggg cgaggcgtcg atggcgcggc gcaggtcagg
     1141 agagcgtgct gccggaaact ggtcgatccg caggggctgc tcgacgcctt ccgacatgcc
     1201 gtccgggtgc gcgtccgcgt agtcgagccc gcgcgcgatc agcgataccc actcgtcgga
     1261 cagcaccccc ttgaccagaa cggctccgtc gcggtgaaag gattcgattt cttccgtcgt
     1321 cacgtttctg agcggcgaag cggacatcag caaacctcca tgggtggcag atcgtgagcg
     1381 gcagtgatgg aaggaagcag aaagcgggcg acgaaccgcc ggcgctccgc cgacgacctg
     1441 ctcctgggct ggggaaatac cgccaaagag ttgaccacac gccggatcca ctcgatcagc
     1501 tcgccccgtt ccacgtcccc acgcagcacc ccggcgcgcg caagcccatc gaggggatcg
     1561 ccgatcatat cgatcacccg atcgttgagc gcacgccgcc cggcggcaag cgccatgttc
     1621 tcggtgcgcg tcctcgggcc gtcgagcaac ctggccaaac ccgcattttc gggcacgttc
     1681 tcagcaatcg ccacgacgat ctccaccagc ctctcaccgg gatcatcaat cggttcgagg
     1741 ttgcctgcaa gccgcgcctc gaaccgatcg aactcgcgaa tgaagaccgc ctgcagcacc
     1801 tcctccatga cggcaaaccg cttgtagagc gtggtgcgcg aaacgcccgc ctcctcggcc
     1861 acgtgcgaca tggtcgtctt cgccactccg tggcgcacga agcaggcctc cgctgcatcg
     1921 atcagggtct gcgtgagcgg gtccagcgcg accgaagccg ccgtggccgg cacggtgttc
     1981 atgcgtgacc gtgcctgagc aggcgcccgg gcgtcgcgcc ggtgcacacg ccgtctgcaa
     2041 aggtgaccgc gccgttgaca atcgtccagc gccagccgtt cgcgcgccgg tcgaggtgcc
     2101 acgcgccgcc gggccaatcc gcaacgcgct cggacggcaa cagctccagc gcctgcagat
     2161 cgtagaccac gatgtccgcg ggcatgccct ccgcgatgaa cccccggtcc gtcagccccg
     2221 ccgtctgcgc ggagtaggcg gagaggcgcc agtgcgcttc ctccagatcc atcaccccgt
     2281 tgtcgcgcac ccacgtggcg agaaactccg tcgggtaggt tccggtggtc aggaacttgg
     2341 tgtgcgcccc gccgtcggac agcccgggca cggcatacgg acagttggcg atgtcgcgca
     2401 tgacctgggg atcgatggtc ctcggctcgc tctgccagcc agccttgagg tcgcaggcga
     2461 tggatacgtc caggaacgcg tcgatgggat gaacgcccat ccccgccgct atctcggcaa
     2521 cggtatggcc ctcccagctc cgcaggtgct cgccttcttc ggtgacgtag gcgagtacca
     2581 ggtcctgaaa cttgacgccg atccgctcct ctcgctgctc ggttccaccg cccgcgatgg
     2641 gcgcctttcc cgccgcccat tcctcatgga gcgcagcacg acgctgggcg tccatcatct
     2701 tcgcctttct ttcaggcacg gtgccgatgg tgagctcccg ccacagcggg gacgagtcga
     2761 acaggttcca gtcctccatg gtgaactggt agtcgatggc gcaggtcacc gcatgagcga
     2821 tgatgcggtt tccctcggca ttggcccggg cgagccacgc aattacgtcc ttgtaagact
     2881 ccgcgggcga gccgtgctga tcgctgcgag cggcgagcac gttgtagatc accggacgcc
     2941 cggacgccgc cgcaagcacg tccgccatct tcggcggacc gatcatctga atgccgccgc
     3001 ggcccagacg acccagcgcc gcggcgaaga actcgatgtc ctcgcggctc atgaggtcgg
     3061 tgatcatggg cgtgccgtcg tgatcgcgct ggacggtgtc ggcgccgatg aactgagccg
     3121 accagccaca ggcgccggcg tccatggctt cggcaagcag gcgctccgcc tcggcctttt
     3181 ctgcccgggt cagcggccgg cctttcgcct cttccaccga gcccgtgagc cacaccatca
     3241 gcgggttgag ccccacgtag gagagcacgt tcaccccttt cggggtgcga tcgaggctgt
     3301 cgagaaattc cggataggtg gtccagtccc aaggcatgcc ctgctgcatg cactccagcg
     3361 gcacggcttc gttacgcgcc atggtgagca tggccctgtc gcgatgctcg ggccgaaccg
     3421 gcgcgaagcc gaacccgcag ttacccacca ccacgctggt gactccgtgc tgcccggaaa
     3481 tccggcacca gggatcccag aagatctggc tgtcgtaatg ggtatgaaga tcgacgacgc
     3541 cgggcgccac gatgagcccg tcggcatcga gctcgcgatc tccgctcccg ggcgctatcc
     3601 gcccgatgac ggcgaccttc ccatcgcgga tgccgacgtc ggcgcgatag ctgggagtgc
     3661 ggcgcccatc gatgagcata ccgttacgga tgacgagatc gagatgcatt ggttgatccc
     3721 tgcagcggcc gaatctgaac aaattatcaa atctgttcac ttgccgtcaa tgcggcgggc
     3781 cgggtttctt ggtctggaat taccacgcgt taccgccatt gtgaggttcc gacccacgtt
     3841 ttatgctcgt tgccctcatc ccgcgaaaaa aacgagaaca cgatgcagga atttcacgtc
     3901 aatgtggcca ctccggatgg cgagatggag tgcttcgccg cgcaccccga tggcgaaggc
     3961 ccgtttgccg ccgtcatcct ttacatggac gcgccgggta ttcgcgaaga gctgcgcgac
     4021 ttctgccggc gcatcgcggg ccaaggctac ttctgtctgc ttcccgatct ctactatcgg
     4081 gagggccgcg tccgcttcga cctgcgcaag ggcgaggaag agctcaagcg catgtttgcc
     4141 gtcggctcgc ggctcaccgt ggaggcggtc atgcgtgata ccggcgggat gctcgcctac
     4201 ctggaagaca accctctggc gcgatcgccg acgggctgca tcggctactg catgagcggc
     4261 cagttcgtcg tggcggcggc gggcaccttt cccggagaga tcgccgctgc cgcgtcgctt
     4321 tacggtaccc gcatcgtcac cgacaagccc gactcacccc acaagctcgc cgagcgcatg
     4381 aagggtgagc tgtacctcgg cttcgccgag cacgaccact gggtcgagga cttcgtcatt
     4441 cccaagctca tcgaggagct cgaccagcat ggggtgaagt acgagctcga agtgcatccc
     4501 ggcacggagc acggcttctg cttcccggag cgcccatcct acgtggaggc cgcggcggag
     4561 aaagtctggg ataccgtctt cgagctgttc gaccgccggc tgaatgccag atgaggcgac
     4621 gggtgcggaa cgctgcatga agggtcttac cgagcgccag ctcaaacggc gtgagcggat
     4681 cctggccgct gcccgcaagc tcatcaccga tcggggctac gacggactga ccatgcgcga
     4741 tctggccgct gagagcgggg tcgcgccgaa aacgctctac caccagttcc acagcaaaga
     4801 gaacctgctg cggttggcgg tggaggagcg ctaccggtat ctgtatcaga tcatcgacga
     4861 cgagcagatc gagcacggca tcgaccggct gttcttcatc atcgacacgg tggcgagcac
     4921 caccgagaac aacctcgcct acgcgaaggc gctcaggcca ctgcttacgt ccgagacctc
     4981 cagcacgttt gcggccatcc gcatgaacac gtaccgcaaa gccatcgacc agatcgaagc
     5041 cgagggggaa gtcgtcgact ggattgacgt cgacatcctc gcggcggtgg tctaccggca
     5101 ggtgaacccc atttacctcg ccccatggta caaggagaca ccctgggcca ccgtcgccag
     5161 aatcgccaag ctggacatct gcctgatcct ggccgcggtg accaccggct acacgcagac
     5221 gacggccgaa aagaccattc gcgatctgca gaagcagctc aaacgatccg gttcccgcta
     5281 catctgagcc ccctcagtcc ttctccggga gcgcgtaggc gatgagatag tcggcgggcg
     5341 gcgacggcga ggtgaagtgc ccgcccgccg cgatgaccac gtactgtcgg cccccggaga
     5401 cgtacgtcat gggaacggcg ttgcccgtcg tcggcatctc atcggcccaa agctcctcgc
     5461 cggtatcgag atcgaaagca cggacgtgcc ggtcggccga cgcggcgatg aagatcagcc
     5521 cggccgaggt gatcgtcggg ccgcccatct gcaccccgcc cttcatcatg gtgaacggcc
     5581 agaacgcctg gtacttcagg ttgcccagcg gcacctgcca ctcgatgtcg cccgtggaca
     5641 ggttcacggc tgacaaggtc ccccacggcg gcttggtgca cggcgcgcca aagcgtgaca
     5701 gaaaaggccg cagcgtcgcg cagtagggcg agccctcctg aggaaagttc tccgtgccgt
     5761 cgcattgatc ccgcgggatc agattgagaa tcatggccac atggtgggta ttggccacca
     5821 tgatctgacg ctccggatcc accgccggcg cgccccagtt ctgaccgccg atcaccgacg
     5881 ggtacagcgg ggtttccttg ttcacggaga tcggcgtgta gatcggcccg gtggtcatgg
     5941 cctcgagcgc atcccgacac cagcccctgt ccaggaacgt gaagccccac gcatcgtcgg
     6001 gggagatgcc cagcttgtgc agcggctcgg gcttgagggg gaagggctgg gtaggcgcaa
     6061 gatactcgcc gggcgccgca ccctcctgag gcaccggccg ctcctccacg ggaaagagcg
     6121 gctgcccggt ctcccgatcg agcacgaacg tcatacccat cttg
//
LOCUS       DBNHOX010000178         6026 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_271128, whole genome shotgun sequence.
ACCESSION   DBNHOX010000178 DBNHOX010000000
VERSION     DBNHOX010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_271128"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..840
                     /gene="dapB"
                     /locus_tag="AC16UZ_03840"
     CDS             25..840
                     /gene="dapB"
                     /locus_tag="AC16UZ_03840"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR6245846.1"
                     /translation="MTRVAVTGAAGRMGKTLIQAIAEAEDLSLGAALERHDSTLLEAD
                     AGELAGVGRAGVAVGASLEDAAEAFDVLIDFSVPAATLAALEVCRERGRGMVIGTTGF
                     DADGLGLIQAAAETIPIMMAPNMSVGVNLCFKLMEIAASVLGDDVDIEVIEAHHRDKI
                     DAPSGTAVRMGEIIAVSLGRDLDEVAVYGRQGITGPRARSTIGFETIRAGDIVGEHTV
                     LFAGNGERIEITHRAQSRMNFAQGALRGVRFLAGRTSGLFDMQDVLGFKGALG"
     gene            916..2043
                     /gene="carA"
                     /locus_tag="AC16UZ_03845"
     CDS             916..2043
                     /gene="carA"
                     /locus_tag="AC16UZ_03845"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR6245847.1"
                     /translation="MVRALLALEDGSVFKGLSIGVEGTSVGEVVFNTAMTGYQEILTD
                     PSYARQIITFTYPQIGNTGINPEDVESDRIWASGMVIRQTPKRASNWRMRQRLEDYLV
                     AEKVVAIADVDTRRLTRLIREKGAMAGCIAAGEAVSEAEAVALARDFPGLAGMDLAQV
                     VTRAEAGSWTQTDWSLESGYGEQTDARFHVVAYDFGVKRNILRLLAARGCRLTVVPAT
                     TPASSVLALEPDGVFLSNGPGDPEPCGYAIEAIGELLDRQIPIFGICLGHQLLGLASG
                     ATTVKMPPGHHGANHPVKDLRDGRVVITSQNHGFAVDADSLPDCLTATHVSLFDGTLQ
                     GIRRSDRPAFGFQGHPEASPGPQDCQYLFDEFIELMESRHA"
     gene            2036..5269
                     /gene="carB"
                     /locus_tag="AC16UZ_03850"
     CDS             2036..5269
                     /gene="carB"
                     /locus_tag="AC16UZ_03850"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR6245848.1"
                     /translation="MPKRTDIDSILILGAGPIVIGQACEFDYSGAQACKALREEGFRV
                     ILVNSNPATIMTDPAMADATYVEPVEWATVARIIEQERPGALLPTMGGQTALNCALDL
                     VREGVLERFGVELIGASQDAIDKAEDRERFDKAMKRIGLETPRSAIAHSMEEAVQVQA
                     QLGFPCIIRPSFTLGGSGGGIAYNAEEFAEICARGLDLSPTNELLIDESLLGWKEFEM
                     EVVRDCVDNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQLLRNASIAVLREIG
                     VDTGGSNVQFAIHPETGRLVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     LDNDITGVTPASFEPSIDYVVTKVPRFTFEKFDQADSRLTTQMKSVGEVMAIGRSFQE
                     SLQKALRGLETGMTGLDPLLEIGGEEAVARLRRELATPGAERIWYVADAFRFGLSLEE
                     VFELSAIDPWFLAEIEDLIAAESQLAGKRLDQIGADALYRLKRQGFSDARLGTLLGAP
                     EQQVREARHSMGIRPVYRRVDTCAAEFPSATAYMYSTYEEECEADPSERPKIMVLGGG
                     PNRIGQGIEFDYCCVHAALAMREDGFETIMVNCNPETVSTDYDTSDRLYFEPVTLEDV
                     LAIVDVEKPKGVIVQFGGQTPLKLVEALEQAGVPIIGTSAEAIDRAEDRQRFQALIRK
                     LGLKQPSNATAVDVETGLKGAREIGFPIVVRPSYVLGGRAMEIVFNEDELARYLLQAV
                     TVSNDSPVLLDRFLDQAVEVDVDAVSDGKQVVIGAIMQHIEQAGVHSGDSACALPPYQ
                     LSADLQDEIREQVRAMALELGVIGLMNVQLAVQEGEIFILEVNPRASRTVPFVSKCIG
                     VSLAKIAARCMAGKTLAEQGFTREIIPEQVNVKESVFPFTKFPGVDPILGPEMKSTGE
                     VMGVGDSFGEAFAKASLGAGVALPSGGRAFISVKDSDKPGLPALGRQLVDLGFQLVAT
                     RGTAKCFAEAGIPSEVINKVGQGRPHVADMLKNEQIDLIINTTEGHQSIRDSAVIRRL
                     ALQNKVCYTTTLTGGEAFCIAIRYSRDHQGETVRRLQDLHADIVGAS"
     gene            5317..5793
                     /gene="greA"
                     /locus_tag="AC16UZ_03855"
     CDS             5317..5793
                     /gene="greA"
                     /locus_tag="AC16UZ_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR6245849.1"
                     /translation="MMPIPMTLEGAEALRKELSERKSVLRAQITQAIAEAREHGDLKE
                     NAEYHAAREQQGFNEGRIQEIENKLADAQVIDVSKITASGKVVFGATVTLIDTESGDE
                     VAYKIVGEDEADIKAGKLSVMSPIARAVIGKTVGDVSVVRTPGGEVEYEIDRVDLI"
     gene            complement(5814..>6026)
                     /locus_tag="AC16UZ_03860"
     CDS             complement(5814..>6026)
                     /locus_tag="AC16UZ_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014087.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HIR6245850.1"
                     /translation="LVAEIERALADHELIKIRIHSPDRSERRAVAEEIAGICEAEVVQ
                     TIGKVCVLFKVNPEPDKALSNLARHG"
BASE COUNT         1093 a         1878 c         2014 g         1041 t
ORIGIN      
        1 ccggagcaac tagggagccg gaagatgacg cgtgttgccg tgacgggtgc ggcgggccgg
       61 atgggcaaga cactgatcca ggccatcgcc gaggcggaag acctgagcct tggcgctgcg
      121 ctggaacgac atgactccac ccttttagaa gccgatgcag gagaactggc tggtgtgggc
      181 cgggcgggcg tcgccgtggg ggcttcgctt gaagatgccg ccgaagcctt cgacgtgctc
      241 atcgacttca gcgttccggc agccaccctg gcggcgctgg aggtctgtcg tgagcgcggc
      301 cgcggcatgg tgatcggcac cacgggattc gatgccgacg ggcttggcct gattcaggcc
      361 gccgcggaga cgatcccgat catgatggcg cccaacatga gcgtgggggt gaatctgtgc
      421 ttcaagctca tggagatcgc cgcctcggtg ctgggtgacg atgtggacat cgaggtgatc
      481 gaggcgcacc accgcgacaa gatcgacgcg ccctccggca cggccgtcag gatgggggag
      541 atcatcgccg tgtcgctggg gcgcgatctc gacgaggtag ccgtgtacgg acgccagggg
      601 atcacgggcc ccagggcgcg cagcaccatc ggtttcgaga ccatccgggc cggcgacatc
      661 gtgggcgagc acacggttct ttttgcaggc aacggagagc gaatcgagat cacccaccgc
      721 gcccagagcc gcatgaactt tgcccagggc gcgctgcggg gcgtccggtt tcttgcaggc
      781 cgaacctcag gcctcttcga catgcaggat gtgctgggct tcaagggtgc acttgggtag
      841 actaccgccc cgaattcaag gcgggatcgg ttctgggcga tctcgccttt ttgcgacctg
      901 ttattggaaa acgatttggt tcgggcactg ctcgctctcg aggacgggag tgtgtttaaa
      961 ggtctgtcga ttggtgtaga aggtacatcg gtcggggaag tcgttttcaa taccgccatg
     1021 acgggctacc aggaaatcct caccgatccg tcgtacgccc gccagatcat caccttcacc
     1081 tacccgcaga tcggtaacac cgggatcaat ccggaggacg tggagtccga tcggatctgg
     1141 gccagcggca tggtcattcg gcagacgccg aagcgggcca gcaactggcg catgcggcag
     1201 cgtctggaag actacctggt cgcggagaag gtggtcgcca tcgcggacgt ggacacccgc
     1261 aggctgaccc ggctcatccg ggagaagggc gccatggcgg gatgcatagc ggccggcgag
     1321 gcggtcagcg aagccgaggc cgtggcgctg gcccgagact ttcccgggct tgctggcatg
     1381 gacctggcgc aggtcgtgac cagggccgag gccggttcct ggactcagac cgactggtcg
     1441 ctcgaaagcg gttacggtga gcagacggac gcgcgttttc acgtcgtcgc ctacgacttc
     1501 ggcgtcaagc ggaacattct ccgcctgctg gcggcgcgcg gctgccgcct gaccgtcgtg
     1561 ccggccacca ccccggcctc cagcgttctt gccctggagc cggacggggt ctttctatcc
     1621 aacggtcccg gcgatccgga gccgtgcggt tacgccatcg aggccatcgg cgagctgctc
     1681 gatcgccaga tacccatatt cggtatctgc ctgggtcatc agctgctggg cctggccagc
     1741 ggggccacta ccgtcaagat gccccccggt caccacgggg ccaatcaccc ggtcaaggac
     1801 ctccgcgatg gccgcgtcgt catcaccagc cagaatcacg gtttcgcggt ggatgccgac
     1861 agcctgcccg actgcctcac ggccacccat gtctctctat tcgacgggac gctgcagggc
     1921 attcgtcgct ccgaccggcc ggccttcggt ttccaggggc atccggaggc aagccccggg
     1981 ccccaggatt gccagtatct gttcgacgag ttcatcgagc tcatggaatc gcgccatgcc
     2041 taagcgtacc gatatcgatt ccatcctcat tctcggcgcg gggcccatcg tgatcggcca
     2101 ggcctgcgag ttcgactact ctggcgctca ggcctgcaaa gcgctgcgcg aggaaggctt
     2161 ccgggtgatt ctcgtcaact ccaatcccgc caccatcatg acggatcccg ccatggccga
     2221 cgcgacgtac gtcgagccgg tggagtgggc caccgtggcg cgcatcatcg agcaggagag
     2281 gccgggtgcg ctgctgccga ccatgggcgg gcagacggcg ctcaattgcg cgctggacct
     2341 ggtcagggaa ggcgtgctcg agcgtttcgg cgtcgagctg atcggtgcca gccaggacgc
     2401 catcgacaag gccgaggatc gggaaaggtt cgacaaggcc atgaagcgca ttggcctgga
     2461 gacaccccga tctgccatcg cgcacagcat ggaggaagcc gttcaggtcc aggcccagct
     2521 cggttttccg tgcatcatcc gcccgtcgtt cactttgggc ggcagcggcg gcggcatcgc
     2581 ctacaacgcg gaggagttcg cggagatctg cgcccggggg ctcgacctgt cgcccaccaa
     2641 cgagctgctc atcgacgaga gcctgctggg ctggaaggag ttcgagatgg aggtggtccg
     2701 cgattgcgtg gacaactgca tcatcgtctg ctcaatcgaa aatctggacc ccatgggcgt
     2761 gcacaccggg gacagcatta cggtggctcc ggcgcagacg ctcacggaca aggaatatca
     2821 gctgctgcgc aacgcctcca tcgccgttct gcgggaaatc ggggtggata ccggcggctc
     2881 caacgtgcag ttcgccatcc acccggaaac cggtcggttg gtggtgatcg agatgaaccc
     2941 gagggtttcg cggtcgtcgg cgctggcttc caaggccacc ggcttcccca tcgccaagat
     3001 cgccgcgaag ctggcagtgg gctacacgct ggacgagctc gacaatgaca tcacgggcgt
     3061 gacccccgcc tctttcgagc caagcatcga ttacgtcgtc accaaggtgc cccgtttcac
     3121 cttcgagaag ttcgaccagg ctgactcacg gctcacgacg cagatgaagt ccgtcggtga
     3181 ggtcatggcc atcggccgca gctttcagga gtccctgcag aaggcgctgc ggggtttgga
     3241 gaccgggatg accgggctcg acccgctcct cgaaatcggc ggtgaggaag ccgtcgcccg
     3301 gttgcggcgc gagctggcca ccccgggtgc agaacggatc tggtacgtcg ccgacgcatt
     3361 ccgtttcggg ctctcgctgg aggaggtgtt cgagctgtcg gccatcgatc cctggtttct
     3421 cgccgagatc gaggacctca tcgccgcgga aagccagctt gcgggcaagc ggctcgatca
     3481 gatcggcgcg gacgcgctgt atcggctcaa acgccagggg ttttcggacg cgcggctggg
     3541 aacgctgctg ggggctccgg agcagcaggt tcgtgaggcg cggcatagca tgggcatcag
     3601 acccgtctat cggcgcgtcg atacctgcgc ggccgagttc ccgtcggcca ccgcctacat
     3661 gtattcgacc tacgaggaag agtgcgaggc ggacccgtcc gagcgaccga aaatcatggt
     3721 gctcggcgga ggaccgaacc gaatcggcca ggggatcgag ttcgactact gctgcgttca
     3781 cgcagcactc gccatgcgcg aggacggctt cgagaccatc atggtcaatt gcaatcccga
     3841 gaccgtctcc acggactacg acacgtcgga ccggctgtac ttcgagcccg tgaccctgga
     3901 ggacgtgctg gccatcgttg atgtcgagaa gcccaagggc gtgatcgttc agttcggcgg
     3961 ccagacgccg ctgaagctgg tggaggccct tgagcaggcg ggcgttccca tcatcggcac
     4021 gagcgcggaa gccatcgacc gtgccgagga tcgccagcgc ttccaggcgc tgatccgcaa
     4081 gctcggcctc aagcagccgt ccaacgccac ggcggtggac gtcgagacgg ggctcaaggg
     4141 cgcccgggaa atcgggttcc ccatcgtcgt gcggccgtcc tacgtgctgg gaggccgggc
     4201 catggaaatc gtcttcaacg aggacgagtt ggcccgttac ctgctccagg ccgtcaccgt
     4261 gtccaacgat tcccccgttc tgctggacag gtttctggat caggccgtgg aggtggacgt
     4321 ggatgccgtc tccgacggga agcaggtcgt catcggcgcg atcatgcagc acatcgagca
     4381 ggcgggcgtg catagcggcg actccgcctg cgcgctgccg ccgtaccagc tttccgccga
     4441 cctgcaggat gagatccgcg agcaggtgcg ggccatggcc ctggagctgg gcgtcatcgg
     4501 gctgatgaac gtgcagctgg ccgtccagga aggcgagatc ttcattctcg aggtcaaccc
     4561 gcgggcttcg cgcacggtcc ccttcgtctc caagtgcatc ggggtttctc ttgccaagat
     4621 cgcggcgcgc tgcatggcgg gaaaaacgct ggcggaacag ggcttcaccc gggagatcat
     4681 tccggaacag gtgaacgtca aggaatccgt cttcccgttt acgaagttcc cgggggtgga
     4741 cccgattctc ggcccggaga tgaaatccac cggcgaggtc atgggcgttg gcgacagctt
     4801 cggtgaggcg ttcgccaagg cctccttagg tgccggcgtc gccctgccct cgggcgggcg
     4861 ggctttcatc agcgtcaagg attccgacaa gcccggcttg ccggcgctcg gccgccagct
     4921 ggtggatctc gggttccagc tggtggctac ccgcggaacg gcgaagtgct tcgccgaagc
     4981 cggcattccc tcggaggtca tcaataaggt cggccagggc cgccctcacg tggccgacat
     5041 gctcaagaac gagcagatcg acctgatcat caataccacg gaggggcacc agtccattcg
     5101 cgactcggcg gtcattcgtc ggctcgcact gcagaacaaa gtgtgctaca ccactaccct
     5161 cacgggcggc gaggctttct gcatcgccat ccgctattcg agagatcatc agggggagac
     5221 ggttcgcagg ttgcaggacc tgcacgccga catagtcggg gcatcctagc ccgacgctca
     5281 gacacgctgc acaaacggga ttgttcgggc agggacatca tgccgattcc aatgacgctt
     5341 gagggcgccg aagcgctccg caaagagctc agcgagcgca agtcggtgct ccgggcgcag
     5401 atcacgcagg ccatagccga agcgcgcgaa cacggcgacc tgaaagaaaa cgccgaatac
     5461 cacgccgcac gcgagcagca gggcttcaac gaggggcgaa tccaggagat agagaacaag
     5521 ctggcggatg cccaggtcat agacgtttcc aagatcaccg ccagcggcaa ggtggtcttc
     5581 ggcgcgacgg tcacgctcat agataccgag tccggcgacg aggtggccta caagatcgtt
     5641 ggtgaggacg aagccgacat caaggcgggc aagctttccg tgatgtcacc cattgcccgg
     5701 gcggtgatcg gcaagaccgt cggggacgta agcgtggttc gcactccagg aggcgaggtc
     5761 gagtacgaga tcgaccgggt tgacctcatc tgagatcgcc gcgcccgtgc ggatcagccg
     5821 tgacgcgcga gattcgacag agccttgtcg ggctccggat tgaccttgaa caggacgcag
     5881 accttgccga tggtctgcac cacctcggct tcgcagattc ccgcgatttc ctctgccacg
     5941 gcccgccgct cggagcggtc gggcgaatgg attcggatct tgatgagctc gtgatccgcc
     6001 agcgcgcgct cgatctcggc gacgag
//
LOCUS       DBNHOX010000179         2476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_657484, whole genome shotgun sequence.
ACCESSION   DBNHOX010000179 DBNHOX010000000
VERSION     DBNHOX010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_657484"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1298)
                     /gene="hemL"
                     /locus_tag="AC16UZ_03865"
     CDS             complement(18..1298)
                     /gene="hemL"
                     /locus_tag="AC16UZ_03865"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIR6245851.1"
                     /translation="MHTDRSRELMRRAALSIPGGVNSPVRAFKGVGGSPIFFERGEGA
                     FLTDVDGNRYIDYVGSWGPMLLGHGNPEVVAAVRAQAEKALGFGAPTELEIELAEQVV
                     DMVPSIEKIRMVNSGTEATMSAIRLARGFTGRDLIVKFDGCYHGHSDSLLVKAGSGVL
                     TLGIPNSPGVPAAIAEQTLTLPFNDLDAVAEAFEQYAGQIAAVILEPVAGNMGCVPPE
                     AGYLEGLRELTGRHGSLLIFDEVMTGFRVSRGGAQQRYGVEPDLTTLGKIIGGGLPVG
                     AFGGRADVMAHIAPEGTIYQAGTLSGNPLAMVAGLTTLRALDDPLYEELEQRTAYFCE
                     ALEASANRHGVSVTINYVCGMFSVFFTNGPVRNFADVARSDTASFNRFFHAMLDGGVY
                     LAPSAFEAGFLSVAHTEDVLDATIEAADQAFATL"
     gene            complement(1326..1760)
                     /gene="hemJ"
                     /locus_tag="AC16UZ_03870"
     CDS             complement(1326..1760)
                     /gene="hemJ"
                     /locus_tag="AC16UZ_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007251893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HIR6245852.1"
                     /translation="MSAGYLWLKALHIIFVVTWFAALFYLPRLFVYHSSSTDAISRDR
                     FKVMESKLYRVIMRPSMLLATGFGLWLVFLGWNAFAGSGWLWVKLTAVVALLGYHHYC
                     ARLVRAFAADADPHSETFYRWFNELPALVLIVVVIMVVVKPF"
     gene            complement(1769..>2476)
                     /locus_tag="AC16UZ_03875"
     CDS             complement(1769..>2476)
                     /locus_tag="AC16UZ_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012861.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR6245853.1"
                     /translation="IGAALGGCIGMIGNLLEPADAISEGFYVMLGMAAMMAAVLQAPL
                     AALMAVMELTLNPNLILPAMLMIVVATLVSSVAFRQRSVFISTLKTLGLEYPPSPVTA
                     HMQRAGVAAIMCEEVAVLPRDCSLDAARRALEQRPLWIAIASDDGETRWVLKSSDLAA
                     HLESEQIDTADVDLLNIPGMRRDATSVDVRCTVDEAQMALQGSPAEALCVRRLGVTAA
                     GSLLGIVTQEDIDTYRG"
BASE COUNT          448 a          805 c          802 g          421 t
ORIGIN      
        1 catgaacggg gctcccctta cagcgttgcg aacgcctgat ctgcggcctc gatcgtggcg
       61 tccagcacgt cctccgtgtg ggcgaccgac aggaagccgg cctcgaaagc cgagggtgcc
      121 aggtagacgc cgccgtcgag catggcgtga aagaaccggt tgaaagatgc cgtgtcggaa
      181 cgggccacgt ccgcgaagtt cctgaccggg ccgttggtga agaaaacgct gaacatgccg
      241 cacacgtaat tgatggttac gctcacgccg tggcgatttg ccgatgcttc cagggcttcg
      301 cagaaatagg ccgtgcgctg ctccagttcc tcgtagaggg gatcgtcgag cgcgcgcaag
      361 gtcgtcaggc ccgccaccat tgccagcggg ttgcccgaga gcgttcccgc ctgatagatg
      421 gtgccttcag gcgcaatgtg cgccatgacg tcggcgcgcc cgccgaacgc gcccaccggc
      481 agccccccgc cgatgatctt tccgagtgtg gtcagatccg gctccacgcc atagcgctgc
      541 tgggcgccgc cgcgcgacac gcgaaatccg gtcatgacct cgtcgaagat gagcaggctg
      601 ccgtggcggc ccgtcagctc gcggagccct tccaggtagc ctgcctccgg cggtacgcat
      661 cccatgtttc ccgccaccgg ctcgagaatg accgcagcga tctgcccggc gtattgttcg
      721 aacgcttcgg ctactgcgtc cagatcgttg aatggcagcg tcagcgtctg ctcggcgatg
      781 gccgcgggta ccccgggtga gttcgggatg cccagcgtca gaacgcccga gcccgccttg
      841 accagcaggg agtcggaatg cccgtgataa cagccgtcga acttaacgat gaggtcgcgc
      901 ccggtgaagc ctctggcgag ccggattgcc gacatggtgg cctcggtgcc cgagttcacc
      961 atccgtatct tctcgatgct cggcaccatg tccacgacct gctccgcgag ctcgatttcg
     1021 agctcggtgg gcgcgccgaa gcctagagct ttctcggcct gggccctcac ggcggcgacg
     1081 acttcggggt tgccatgccc taacaacatc gggccccagg agccgacgta gtcgatgtaa
     1141 cggttgccgt ccacgtctgt gagaaacgcg ccctcacccc gctcgaagaa aatgggcgag
     1201 ccgccgacgc ccttgaacgc gcggacgggg gagttgacgc cgccgggaat ggacagggcc
     1261 gcccgccgca tcagttccct cgacctgtct gtgtgcatcg ctcgacccct atcgcgtgac
     1321 gggagctaga agggtttgac caccaccatg atgaccacca cgatcagcac gagtgcgggc
     1381 agctcattga accaccggta gaacgtctcg ctgtgggggt cggcatctgc cgcaaaggct
     1441 ctgacgagcc gcgcgcagta atgatgataa ccgagcagtg cgaccaccgc agtcagtttc
     1501 acccacagcc agcccgagcc ggcgaacgcg ttccagccga ggaagacaag ccagaggccg
     1561 aagccggttg ccagcagcat ggagggccgc atgatgacgc ggtagagctt gctctccatc
     1621 accttgaatc ggtcgcggct gatcgcatcg gtgctgctgg agtggtagac gaacagcctc
     1681 ggcaggtaga agagggccgc gaaccaggtc acgacgaaga tgatgtgaag ggccttcagc
     1741 cacaggtagc cggcgctcat cgtttggatc aacctctgta ggtgtcgatg tcttcctggg
     1801 tgacgatgcc cagcaggctc ccggccgccg tcacgcccag ccggcgcacg cacagcgctt
     1861 cggcagggct tccctgcagg gccatctggg cttcgtccac ggtacagcgc acgtcgacgc
     1921 tggtcgcgtc cctacgcatg ccgggaatat tgagcaggtc gacgtcggcg gtgtcaatct
     1981 gctcggattc taggtgggcg gccaggtcgc tcgacttgag gacccagcgg gtctcgccgt
     2041 cgtccgatgc gatcgcaatc cataacggcc tttgctcgag tgcccttcgc gcggcgtcga
     2101 gagagcagtc acggggaagc accgccactt cttcgcacat gatggccgcc acgccggcgc
     2161 gctgcatgtg cgccgtgacc gggcttggcg ggtactcgag ccccagggtc ttcagggtgg
     2221 agatgaacac cgatcgctgc ctgaacgcca cgctgctcac cagcgtcgcc accacgatca
     2281 tcagcatggc gggcagtatt agattcgggt tcagggtgag ctccatgacg gccatgagcg
     2341 cggcgagcgg agcctgaagc acggcggcca tcatggccgc catgccgagc atgacgtaga
     2401 acccctcgga gatcgcatcg gccggctcga gcaggttgcc gatcatgccg atgcagccgc
     2461 cgagagccgc gccgat
//
LOCUS       DBNHOX010000180         2422 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_979063, whole genome shotgun sequence.
ACCESSION   DBNHOX010000180 DBNHOX010000000
VERSION     DBNHOX010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_979063"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="AC16UZ_03880"
     CDS             complement(<1..578)
                     /locus_tag="AC16UZ_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245854.1"
                     /translation="MATALPKIQDQNDLIQANAVDPATQAVTAAARAAANSRDLGAGS
                     GSAGLPNPTFQRNSQAPAQAAAATQINRDLTSSPSMPNDNFYRVPGAGERVNAAAQIA
                     RDIAPAPQVPMGDAGPATFVAERGSAGMPNPTFERVAAPASAPAPASAPAEGGVGTFL
                     RNMAGAVQTAAKAGQAQTAAGVAEAGFDPSTPP"
     gene            complement(586..1323)
                     /locus_tag="AC16UZ_03885"
     CDS             complement(586..1323)
                     /locus_tag="AC16UZ_03885"
                     /inference="COORDINATES: protein motif:HMM:NF049196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adaptor protein"
                     /protein_id="HIR6245855.1"
                     /translation="MRTFGDALEALRLDILRDAPERALWRDEALVDCLFQAHVEFAER
                     SLYFRDATSAITKVALREGVDLYPLDASILTVMSARIDGDTFDLTRVGHPQLNGSAPE
                     SDTAGWLDRVNSQTPAGRPTAYSTDEGVDGVACATVLRVFATPTAAESGLVVRMRVAR
                     LPRILPSMDTLDAEIEMPLQYRRGLLYGAAAYAYDTHDSDAGDAGEAARMRGKFDTII
                     AQAREGMRTKMHQPLTWGFGRGGFNHS"
     gene            complement(1327..1689)
                     /locus_tag="AC16UZ_03890"
     CDS             complement(1327..1689)
                     /locus_tag="AC16UZ_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245856.1"
                     /translation="MIEPNLPPETDNPEVGELASPPAPKGKGKSAKADPAVHPRFAAE
                     KRIKIVLEENDNIPPTGLFIGINGQSFMLRPGEEASVPASVVAVLDDAVEENPRTDAN
                     GNVLDYRKKLRFPYRMVG"
     gene            complement(1689..1925)
                     /locus_tag="AC16UZ_03895"
     CDS             complement(1689..1925)
                     /locus_tag="AC16UZ_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245857.1"
                     /translation="MTSVRVEKLENGYEVEVCDPKVKAENEKPKSQWKDPWKKYAFST
                     SKEVIKFVTAHLDSMPKSADEIYDEAFKEASKEK"
     gene            complement(1989..2318)
                     /locus_tag="AC16UZ_03900"
     CDS             complement(1989..2318)
                     /locus_tag="AC16UZ_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245858.1"
                     /translation="MGIATSQVNNSAGIVNRTIQYFTTDAAAAANTDFSFGFKPRMVQ
                     FVNLTDRITNEWYEGMAAGYALKTVAAGTRTLETTGGITVNSDGTVTIPAALMVASKS
                     FAVVAEG"
BASE COUNT          362 a          763 c          836 g          461 t
ORIGIN      
        1 ggcggggtgc tgggatcgaa cccggcctca gcaacaccgg cggctgtttg cgcttgccct
       61 gccttcgccg ccgtttgaac ggccccagcc atgttacgca ggaacgtgcc tacaccaccc
      121 tcggccggtg cagatgcagg tgcaggtgca gatgcaggtg cagccacgcg ctcgaacgtc
      181 gggttgggca taccagcgga gccccgctct gcgacaaagg tggccgggcc ggcgtccccc
      241 atagggactt gcggggccgg ggcaatgtcg cgcgcaatct gcgcggctgc gttcacgcgc
      301 tcgccggcgc ccgggacacg gtaaaagttg tcgttgggca tcgacggcga cgaggtgagg
      361 tcgcggttga tctgcgtggc tgcggcggct tgcgcggggg cctgcgagtt acgctggaac
      421 gtcgggttgg ggagccctgc ggagccggaa ccggcgccca gatcgcgcga gttcgcggcg
      481 gcacgcgcgg cggcggtaac tgcttgggtg gctggatcga ccgcgttggc ctgaatcagg
      541 tcattctgat cctgaatttt ggggagggcg gtcgccatac ttacctcagc tgtggttgaa
      601 gccgcctcgt ccgaatcccc acgtcagcgg ctggtgcatc ttagtgcgca tcccctcgcg
      661 ggcctgcgcg attatggtgt cgaatttgcc ccgcatacgc gcagcttcgc cggcatcgcc
      721 ggcatccgaa tcatgcgtat catacgcata cgctgcggcg ccgtacagca gcccgcggcg
      781 gtactgcagc ggcatttcaa tctcggcgtc cagcgtgtcc atcgacggca ggatgcgcgg
      841 cagtcgcgca acccgcatgc gaacgaccag ccccgactcc gcggccgtgg gggtcgcaaa
      901 cacgcgcagc accgtggcgc atgcaacgcc gtcaacgccc tcgtccgtgg agtacgccgt
      961 cgggcgcccc gcgggggtct ggctgttgac gcggtcgagc cagcctgcgg tgtcggactc
     1021 cggggcggag ccgttgagct gcgggtgccc gacccgggtc aggtcgaagg tgtctccgtc
     1081 gatgcgcgcc gacatcaccg tcaggatgga ggcatcgagc gggtacaggt cgaccccctc
     1141 ccgaagtgcc accttggtga tagccgaggt ggcgtcacgg aagtacagcg aacgttcggc
     1201 gaactcgacg tgtgcctgaa acaggcagtc gacgagcgcc tcgtcgcgcc acagcgcccg
     1261 ctccggggcg tcccgcagga tgtcgagccg gagggcttcg agggcgtcgc cgaacgtgcg
     1321 catgggttag cccaccatcc ggtacgggaa gcggagcttc ttgcggtagt cgagcacgtt
     1381 gccgttcgcg tcggtgcgcg ggttctcctc gacggcgtca tccagcaccg cgacgaccga
     1441 ggccggcacc gaggcttcct cacccgggcg cagcatgaag gactggccgt tgatgccgat
     1501 gaacagcccc gtcgggggga tgttgtcgtt ctcttcgagg acgatcttga tgcgcttctc
     1561 ggcggcaaag cgcgggtgca cggcggggtc cgccttggcg ctcttgcctt tgcccttggg
     1621 tgccggcggc gaggccaact cgcccacttc ggggttgtcg gtttccgggg gcaggttcgg
     1681 ctcgatcatc atttctcctt gctcgcctct ttgaaggctt cgtcgtagat ttcatccgcg
     1741 gatttgggca tcgaatccag atgcgccgtc acgaatttta tcacttcttt ggacgtggag
     1801 aaggcgtact tcttccacgg gtctttccac tgagacttgg gcttctcgtt ctcggctttg
     1861 accttggggt cgcagacctc gacttcgtag ccgttctcca gcttctcgac gcgtaccgag
     1921 gtcatggagc ctccacaggc ggtgacgtgt tccatcgtgg cgagggggcc gaagccccct
     1981 ctcctgcctt agccttcggc gacgacggcg aacgacttgc tcgccaccat caacgcggcc
     2041 gggatggtca cggtgccgtc ggagttgacg gtgatcccgc cagtcgtctc cagcgtgcgc
     2101 gtgccagcgg cgacggtctt gagcgcgtag ccggcggcca tgccctcgta ccactcgttg
     2161 gtgatgcggt ccgtgaggtt cacgaactgc accatgcgcg gcttgaagcc gaagctgaag
     2221 tcggtgttgg ccgccgcagc ggcgtcggtg gtgaaatact ggatcgtgcg gttgacgata
     2281 ccagcggagt tgttgacctg cgaggtagcg atacccattt caatctcctg tcagagggtg
     2341 gcggggggcc gaggcccccc tacgcattag gcggtggcgg tgacttcgcc gcggatcagg
     2401 aacgcatcct gcaggatcac tg
//
LOCUS       DBNHOX010000181         3404 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_721828, whole genome shotgun sequence.
ACCESSION   DBNHOX010000181 DBNHOX010000000
VERSION     DBNHOX010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_721828"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1313)
                     /locus_tag="AC16UZ_03905"
     CDS             complement(144..1313)
                     /locus_tag="AC16UZ_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6245859.1"
                     /translation="MDIELSTEDVEFRAEVRAFLKDNAYEAGTDYNEWRNGWFEKAAE
                     KGGWDVPKWPEAFGGPGWSPTQHYIWEQETAVATLPWDLPFGIGMLAPILMSYGNEEQ
                     QKRFLPDIRARKVNWCQGYSEPGAGSDLASLKTQAVLSEDGSHYTVNGTKIWTTVAHI
                     ADWIFCLTRTDDSGKKQEGITFLLFPMKQPGVEVKPIITLGGAHSVNTVFLTDVKVPV
                     ENRIGEEGKGWTYAKGLLQHERTGLAGISRSIVALENLKAQAATVIRGNGTLMDDPGF
                     KGKVAELEVDLMAVEFTELRSLASASAGAAPGPDSSILKLKGTEIQQRIQKLTVAAGG
                     IYSAAWGARPVGHGFAKGGMAGYLASRAYTIYGGASEVQKDVIAKNVLGIKRSGR"
     gene            1399..2001
                     /locus_tag="AC16UZ_03910"
     CDS             1399..2001
                     /locus_tag="AC16UZ_03910"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6245860.1"
                     /translation="MAHERITRATTPARRASVLDAALACFSERGLEGTTVSDIQSAAG
                     CSVGSIYHHFGSKEGIAEELFILGVQRLNQRMLKRLRRCKTGEESVRAVVGEYVRWAT
                     RESAMARYLHSRDVSFSADARERLRDIHREYVEALFAWFAPFVETGEMRRLPVQVYIP
                     LISGPIQEYVRRWLSGQHTRSPQQVEALFADAAWNAVKAR"
     gene            2024..2812
                     /locus_tag="AC16UZ_03915"
     CDS             2024..2812
                     /locus_tag="AC16UZ_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6245861.1"
                     /translation="MSYETLLFEVADGVGTITINRPEAFNAMSPLCAKEFADVAVRCD
                     DDPDVRAVVLTGAGKAFCAGGDLGAFASAGSSAKSLLKVMAGDLHMGLSRLARGNAPV
                     IAAVNGTAAGAGFSLVMAADLAIAAHSAVFTMAYTNAGLSPDGSSTFYMPRKIGDRRT
                     RELMLTNRLLKAEEALAWGVVNQVVADDQVVAEAMKLAQKLAAGPTLAFGAVKTLLNG
                     TFDQTLESQMELEARAIAELSVTADGQEGINAFMAKRKPEFRGA"
     gene            complement(2883..>3404)
                     /locus_tag="AC16UZ_03920"
     CDS             complement(2883..>3404)
                     /locus_tag="AC16UZ_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6245862.1"
                     /translation="SDTIARGQYLVTLLGCGRCHTEGYLTGDKATGPYLGGSRVGIAY
                     TAYSMDDTHPGVVFAGNLTPDDATGLGTWSEEEIVQAMTVGVAKSGHERLTVMPWTNY
                     GALKEKDVRAIARYLKSLSPVRWPIPEAIPEGDPIDEPYVRFGVYKFYPHGPVEAINL
                     PVPTNEPSPEGVQ"
BASE COUNT          555 a         1068 c         1149 g          632 t
ORIGIN      
        1 ctcggccatg gtcgcccagt agtcgctgct gaagccggta tcgctgccgg caagctcgcg
       61 gcgggtctcc agcgcgtagt tttcgtcgac aaaccgctcc acgctgtcgc gcagcagctg
      121 ctgttccgaa gaaagttcga agttcatcgg cccgacctct tgattcccag cacgttcttg
      181 gcgatgacgt ctttctgcac ctcgctcgcc ccgccgtaaa tggtgtaggc gcggctcgcc
      241 aggtagccgg ccatgccgcc cttggcgaaa ccgtggccga cgggtcgtgc cccccaggcg
      301 gcggagtaga tgcccccggc tgccaccgtg agcttctgga ttcgctgctg tatctcggtg
      361 cccttgagct tgagtatgga ggagtcgggc ccgggtgccg cgcccgccga ggccgaagcg
      421 aggcttcgca gctcggtgaa ctccaccgcc atgaggtcga cctccagctc ggcgaccttg
      481 cccttaaagc cggggtcgtc cattagggtt ccgttgccgc ggatcaccgt tgcggcctgg
      541 gctttcaggt tctccaaagc cacgatggag cgggaaatcc cggcaaggcc ggtgcgttca
      601 tgctggagaa gtccttttgc gtaggtccag cccttgccct cctcgccgat gcggttttcc
      661 acgggaacct tcacgtcggt gaggaacacc gtgttgacgc tgtgggcacc tccgagggtg
      721 atgatgggtt tgacctctac gccgggctgc ttcatgggga aaagcaggaa ggtgattccc
      781 tcctgcttct tgccggaatc gtcggtgcgg gtcaggcaga agatccagtc ggcgatgtgg
      841 gctacggtgg tccagatctt ggtgccgttg acggtgtagt ggctgccgtc ctctgacaga
      901 accgcctggg tcttgagcga ggctaggtcg gagccggccc cgggctccga gtagccctgg
      961 caccagttga ccttacgggc ccggatgtcg ggcagaaacc gcttctgctg ctcctcgttg
     1021 ccgtaggaca tgagaatggg agccagcatg ccgatgccga agggcaggtc ccagggcagg
     1081 gtggccaccg cggtttcctg ttcccagatg tagtgctggg tgggtgacca tcccggtccg
     1141 ccgaaggcct ccggccactt gggcacgtcc cagccgcctt tctcggcggc tttctcgaac
     1201 cagccgttgc gccactcgtt gtaatccgtg ccggcttcgt aggcgttgtc tttcaggaac
     1261 gcgcggacct ccgcgcggaa ctcgacgtcc tccgtcgaga gctcgatgtc catggctgaa
     1321 tcctccattc gagaagggcc ggatattaac gctgattaga atcttttact agaactgaat
     1381 tctagttacc atgacgccat ggcccacgag agaatcaccc gagcgactac cccggcgcgg
     1441 cgcgcgagcg tcctggatgc ggcgctcgcc tgcttttccg aacgcggtct cgaggggacc
     1501 acggtcagcg acatccagag cgccgccggc tgcagtgtgg gcagcatcta tcatcatttc
     1561 ggcagcaagg aaggcatcgc ggaggagctg ttcatcctcg gcgtgcagcg tctcaaccag
     1621 cgcatgctca agcgcctgcg gcggtgcaag accggggagg aaagcgtacg cgcggtggtg
     1681 ggcgagtatg tccgctgggc cacgcgggag tctgcgatgg cccgctatct gcactcacgc
     1741 gacgtcagct tctcggcgga cgcgagggag cggctgcggg acatccaccg ggagtatgtg
     1801 gaggccctgt tcgcctggtt tgcgccgttc gtggaaaccg gcgagatgcg ccgcctgccg
     1861 gtccaggttt acatccccct catcagcggt cccatccagg agtacgtgcg acgctggctc
     1921 tccgggcagc acacccgctc gccgcagcag gtggaggcgc tgtttgcgga tgcggcctgg
     1981 aatgcggtaa aagctcgata accgcacggg ttaaggcgta gagatgagtt acgagacgtt
     2041 gctgttcgaa gtggccgacg gcgtgggaac gatcaccatc aaccggcccg aggcattcaa
     2101 cgccatgagc ccgctgtgcg cgaaggagtt tgctgacgtg gccgtgcgct gcgacgacga
     2161 tcccgacgtg cgcgcggtgg tgctcacggg agcgggcaaa gcgttctgcg ccggtgggga
     2221 cctgggcgcc ttcgccagcg ccgggtcgag cgcgaagagc ctgctcaagg tgatggccgg
     2281 ggacctgcac atggggctct cgcggcttgc ccggggcaac gcgcccgtca tcgcggccgt
     2341 gaacggcacg gcggcggggg cgggattcag cctggtcatg gccgccgatc tggccatcgc
     2401 tgcacacagc gcggtgttca ccatggccta caccaatgcc ggcctttctc ccgacggcag
     2461 ctcgactttc tacatgccgc ggaaaatcgg cgaccggcga acgcgagagc tcatgctcac
     2521 caaccggctg ctgaaagccg aggaggcctt ggcctggggg gtcgtcaacc aggttgtggc
     2581 ggacgatcag gtggttgccg aggccatgaa gctcgcgcag aaactggcgg cggggccgac
     2641 gcttgcgttc ggtgcggtga aaaccctgct caacggcacg ttcgatcaga ccctcgaatc
     2701 gcagatggag ctggaagccc gagcgatcgc cgagctatcc gtgacagccg acggccagga
     2761 gggcatcaac gccttcatgg ccaagcgcaa gccggagttc cgcggcgcct agatcgcggc
     2821 gcgcccggaa tctgcaaccc cgtcggccct ggagccgcac gaaagcccgc ggcttgttgc
     2881 ggtcattgca ccccttcggg cgacggctcg ttggtgggaa ccggcaggtt gatggcctcc
     2941 accgggccgt gggggtagaa cttgtagacg ccgaagcgta cgtagggctc gtcgatcgga
     3001 tctccctcgg gaatggcctc ggggatcggc cagcgcacgg gggagaggct cttcagatag
     3061 cgggcgatgg cccggacgtc cttttccttg agcgccccgt agttcgtcca cggcatcacg
     3121 gtcaggcgct cgtgaccgct cttggctacg cccacggtca tggcctggac gatctcctct
     3181 tcgctccagg ttcccagacc ggttgcgtcg tcgggggtca ggttgccggc gaagaccacc
     3241 cctggatggg tgtcgtccat gctgtacgcc gtgtacgcga tgcccacgcg ggaaccgccg
     3301 agatagggac ccgtggcctt atcgccggtg aggtagccct cggtatgaca ccgcccgcag
     3361 cccagcaggg tgaccaggta ctggcctctg gcaatggtgt ccga
//
LOCUS       DBNHOX010000182         3393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_529062, whole genome shotgun sequence.
ACCESSION   DBNHOX010000182 DBNHOX010000000
VERSION     DBNHOX010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_529062"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="AC16UZ_03925"
     CDS             complement(<1..868)
                     /locus_tag="AC16UZ_03925"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6245863.1"
                     /translation="MSEIDEKEVRQEVGTWLEANWDPQLGLLEWRNILVDSGWGMPHW
                     PTQWHGRGLPVGLTPAVDDELASVNAVSVAKSGIRLLAAATLLEHGSDMHREKFLRRI
                     LTGEDTWCQLFSEPGSGSDLAGATTRADFDGTSWIINGQKVWTTSAHHAQWGLLLART
                     DWDVPKHQGLSYFVLDMSQPGVEVQPLRQMNGHASFNQVFITDAKVAPEFQVSTTGNG
                     WAVAMTTLAHERRGADGLRHAARAKDLPGRIYEEERAELATVMEPYKWYPQRAGRIDL
                     VIDRASATGALAQ"
     gene            complement(880..2010)
                     /locus_tag="AC16UZ_03930"
     CDS             complement(880..2010)
                     /locus_tag="AC16UZ_03930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6245864.1"
                     /translation="MSFSFTDEQEQFREATRRFLQDKSPTTEVRRLMETSEGFDEAVW
                     KQLSGELALPGIHIPEQYGGAGFGPVELGIIMEELGRSLLCAPYFGSSVLAATAILEA
                     ATEDWKCELLPALAAGEQLGALAVTETGGRWDSDGIATVARDSGAGFSLTGTKSFVVD
                     GLTAGFLIVAARTPDTGGEAGVGLYLVRPDDAGVTRTPLETMDATRKLARIDLLDAPA
                     RPLGEPGADWPALQRTLDLAAVALANESVGGAQVMLDSSVEYSKIRMQFGRAIGSFQA
                     IKHRCADMLVEVELAKSAAYYAAQAAASGDPELPALACLAKAAASDAFVSTAASCIQI
                     HGGIGFTWDHDTHLWFKRAKSSEVFLGTPDHHRELYLQRTGV"
     gene            complement(2029..3213)
                     /locus_tag="AC16UZ_03935"
     CDS             complement(2029..3213)
                     /locus_tag="AC16UZ_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6245865.1"
                     /translation="MSELSEFRNQTRAWLETSCPAGARGPGQIPWGSSKIPLESDVRL
                     WLERMAERGWTVPTWPKEYGGAELSRDQYMVLIDELKRINARTPLTGRGVNYIGPTIL
                     EFGTHEQKERWLPGIARGEGGWAMGYSEPGAGSDLASLSTRAELQGDQYVLNGRKTWT
                     SDAMDCDYIFVLARTDPDRPKHQGISLLLVDMNQPGVQVRPIRLLSGASPFNETTFEN
                     AVARADDVIGEVNAGWTVGKRLLQFERSTHAGINISGSQGGRSAESNLAERLKQFVPS
                     RDGKLQDPELRERLVRYEQNGHAQRLTQKRVIEELKARAPGYASSAVKLTGALHTQEG
                     DELLLAAMGTQGIVWDGPGLERSDSEALRTWLRQKSLTIAGGTKEVQLNIIAKRVLGL
                     PD"
BASE COUNT          514 a         1136 c         1099 g          644 t
ORIGIN      
        1 gctgcgccaa ggcgcccgtg gctgatgccc gatcgatgac tagatcgatg cgaccggcgc
       61 gctggggata ccacttgtac ggctccatca ccgtggcgag ctctgcccgc tcctcctcgt
      121 agatgcggcc tggcaggtct ttcgcgcgcg cggcgtgacg cagcccgtca gcgccgcgac
      181 gttcgtgggc gagcgtggtc atggctacag cccagccgtt gccggtcgtg gatacctgga
      241 actccggtgc gaccttggcg tcggtgatga agacctgatt gaaggacgcg tgaccgttca
      301 tctgcctgag aggctgcacc tccaccccgg gctgggacat gtcgaggacg aagtaggaca
      361 gcccctgatg cttcggcacg tcccagtctg tgcgtgccag cagcaatccc cactgggcgt
      421 gatgagcgct ggtggtccac accttctggc cgttgatgat ccagctggtc ccgtcgaagt
      481 cggcgcgggt ggtggcaccg gcaaggtccg acccgctgcc gggctcgctg aagagctggc
      541 accaggtgtc ctcgccggtg aggatccggc gcaggaactt ctccctgtgc atgtcgctgc
      601 cgtgctcgag cagcgtggcc gccgccagaa gcctgatgcc cgactttgcc acgctcaccg
      661 cgttgacgga cgccagctcg tcgtccacgg ccggcgtcag ccccaccggc agtccccggc
      721 cgtgccactg ggtgggccaa tggggcatgc cccaccccga gtccacgagg atgttgcgcc
      781 actcgagcag gcctagctgg ggatcccaat tcgcttccag ccaggtcccg acctcctgac
      841 gtacttcctt ttcgtcgatt tcgctcatgg tcaacgtcct cagactccgg tccgctgcag
      901 atacagttcc cggtggtggt cgggggtgcc cagaaagacc tctgagctct tggcccgttt
      961 gaaccacagg tgcgtgtcgt gatcccacgt gaagccgatg ccgccgtgga tctggatgca
     1021 gcttgccgcc gtgctcacga acgcatcgct tgcagcggct ttggcgaggc aggcgagggc
     1081 cggcaattcg gggtcgcccg aagcggcggc ctgggctgcg tagtaggcgg cggactttgc
     1141 cagctccact tccacgagca tgtcggcgca ccggtgcttg atggcctgga aggatccgat
     1201 ggcgcggccg aactgcatgc ggattttgga atactccacg gagctgtcca gcatgacctg
     1261 ggctccgccc acggattcgt tggccagggc caccgccgcg aggtccagcg ttcgctgcag
     1321 ggcgggccag tccgcgcctg gctctcccag cggccgtgcg ggcgcgtcga gcagatcgat
     1381 gcgtgcgagc ttgcgtgtgg catccatggt ttccaacggg gtccgggtga ccccggcatc
     1441 gtctggcctg acgagataca accctacgcc ggcctcaccg ccggtgtccg gtgtccgggc
     1501 cgccacaatc aggaagccgg ccgtcaggcc atcgaccacg aagctcttgg tcccggtcag
     1561 cgagaagccg gcgccgctgt cccgggccac ggtcgcgatg ccgtcgctgt cccatcgccc
     1621 gccggtttcg gtgaccgcca gcgcgccgag ttgctcgccc gctgccagcg cgggcaggag
     1681 ctcgcacttc caatcttccg tagcggcctc gagtatggcg gttgcggcca gcacgctgga
     1741 gccgaagtac ggcgcgcaca ggagggaccg accgagctcc tccatgatga tgccgagctc
     1801 cacgggaccg aatccggcgc cgccgtactg ctccggaatg tgaatacccg gcagcgccag
     1861 ctccccggac agctgcttcc ataccgcctc gtcgaaaccc tcagaggtct ccatcaggcg
     1921 cctgacctcg gtggtcgggg acttgtcctg cagaaatcgc cgggtggctt cccggaactg
     1981 ctcctgctcg tcggtaaagc tgaaggacat ctcgcaaact cctcgaggtc aatccggcag
     2041 gccgagaacg cgtttcgcga tgatgttcag ctgcacttct ttggtcccgc cggcaatggt
     2101 cagggatttc tggcgcagcc aggtgcgcag tgcctcgcta tcgcttcgtt cgagtcctgg
     2161 cccatcccag acgatcccct gggtccccat ggcggcgagc aggagctcgt ccccttcctg
     2221 ggtgtgcagt gcgcccgtca gcttcacagc ggaagaggca taccccggcg cgcgcgcttt
     2281 tagctcctcg atgacgcgct tctgggtcag gcgctgggcg tggccgttct gctcgtagcg
     2341 aaccagccgc tcgcgcagct cgggatcctg cagcttgccg tcccgactgg gaacgaactg
     2401 cttgaggcgt tctgccagat tgctttcggc gctgcggccg ccctgggagc cggagatgtt
     2461 gatacctgcg tgggtggaac gttcgaactg cagcagacgt ttccccactg tccagccggc
     2521 gttcacctcg ccaatgacgt cgtcggccct ggccacggcg ttctcgaacg tggtctcgtt
     2581 gaagggtgag gcgcccgaaa gcaggcgaat ggggcggacc tgcacgccgg gctggttcat
     2641 gtccacgaga aggagactga ttccctggtg tttcgggcgg tcgggatccg tgcgggcgag
     2701 aacgaagatg tagtcgcagt ccatggcgtc ggatgtccac gtcttgcggc cgttgagaac
     2761 atactgatcg ccctgaagct ccgcccgggt gctcaggctg gccaggtcgg atccggctcc
     2821 gggctcggag taccccatgg cccatccgcc ttctcccctg gcgattcccg gcagccagcg
     2881 ttctttctgc tcgtgcgtac cgaattcgag tatggtcgga ccgatgtagt tcacgccccg
     2941 tccggtcagc ggcgtgcggg cgttgatgcg tttgagctca tcgatcagca ccatgtactg
     3001 atccctgctc agctcggcgc caccgtactc cttcggccag gtcggaaccg tccagccgcg
     3061 ttccgccatg cgctcaagcc acaggcgcac gtcgctttcc agcgggatct tgctgctgcc
     3121 ccacggtatc tggcccggcc cgcgggcgcc ggcggggcag ctggtttcca gccatgcccg
     3181 cgtctggttg cggaactcgc tcagctcgct catctggttt ctctcaagcg ctcgatccag
     3241 cgatgcacgc cggttgaaac ggcgatcacg gcgaccgatt aacgctacaa ttcatccatg
     3301 aaaattgcca cctggaatgt caacgggctg aacgcccgac aggagttcgt cggtttgtgg
     3361 ctggcccacc ggcagcccga cctggtcggg ctg
//
LOCUS       DBNHOX010000183         4590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_79198, whole genome shotgun sequence.
ACCESSION   DBNHOX010000183 DBNHOX010000000
VERSION     DBNHOX010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_79198"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="AC16UZ_03940"
     CDS             <1..1196
                     /locus_tag="AC16UZ_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HIR6245866.1"
                     /translation="RANPLYETFLQAAEQAGHALSDDLNGYRQEGFGDLEMTVRDGVR
                     CSAALAYLAPAVSRSNLTVRSGSPVQRLNVESGRCEGLTYRHRGATHTAVAREEVVLA
                     AGAIGSPQLLMLSGIGHGEALEPHGIPVRHELPGVGANLMDHLEIYFQQACTQPVSLN
                     RWLNPLGKGVIGARWLLTASGPGASNHFETGGFVRSAADVPWPDVQFHFLPAAVSYDG
                     RSVADADGFQVHVGPMLSPSRGRLTLRSADPDVAPVISFNYMSCEEDWQVFRRAIRLS
                     RELFAQRAFDPYRGVEIAPGSGADSDGAIDAFVRRSTESAYHPCGTCRMGNDPMAVVD
                     SECRVRGIQGLRVVDASIFPHITNGNLNAPTIMTAEKAADAILGRRLAAEALGYFDAP
                     ASPTR"
     gene            1220..2044
                     /locus_tag="AC16UZ_03945"
     CDS             1220..2044
                     /locus_tag="AC16UZ_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245867.1"
                     /translation="MLGLVLLNGIGVCSMAQADEKPVPRDAGLEFVYDDGFTESERRK
                     LEAWIAEVAGALEVLVGPLPMDVRFRFARHEGAGPVPWANTLRGRRQGVRLYVDPTQP
                     VEAFRNDWTAPHELSHLVLPFVGREHAWFSEGFASFMQFRVMRIMDVLDDEAMAARYS
                     ERLDTAAAAYSQPNRRFVEAVPQLRAEGLYPTIYWGGASYFLEMEERLKRKGASLVGV
                     LAAYLACCRRNEDDLEDLVARLDDLAGSTTFSDGLTGFRRVVGFPPHRHLQALAPR"
     gene            complement(2041..2730)
                     /locus_tag="AC16UZ_03950"
     CDS             complement(2041..2730)
                     /locus_tag="AC16UZ_03950"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6245868.1"
                     /translation="MYRLHGATSSPFVRKTWIFMKEKGLEFEHCELDPLDKSQRFLAM
                     NPLGRIPVLEEPDGNFIADSSVICDYLERCHPGPALYPSNNRDRARALWLEEYADTAL
                     NQVCARIFWMHIIIPFRTGRPADQGEIDAFKEEAYPGVFDYLDRAAPETGDGMVGGRF
                     GIADMALAAPVRLLDLAGDPLDATRWPRFDAHYQRVVARPSAQSIVAKDLATTEVWRT
                     TGNAPASTQSG"
     gene            complement(2768..3676)
                     /locus_tag="AC16UZ_03955"
     CDS             complement(2768..3676)
                     /locus_tag="AC16UZ_03955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6245869.1"
                     /translation="MMRIGISVSSSYQVDDPREGARNMIERARAARDAGLDSLFVGDH
                     HVTPFTYYQNTPILGRMLAEWSEMPFGALYLLPLWHPVVLAEQIATLASIAPGRFIMQ
                     CGLGDDRQGKAMGVDMSRRVGMFRASLEVMRALWRGESVDEERYWHLCGARIAPLPPE
                     PVEVWIGALVQPAIRRAAQLGEGWLASPNLTPAQTADAANVYRQACAEFGRSPAAVAL
                     RRDIFVGATSQAARATVTPYVEAGYRGMSEDALLFGSPGEVADQLRSFADQGYTDVIV
                     RNLSARQDESLATIERLAEVKALLTG"
     gene            complement(3690..4250)
                     /locus_tag="AC16UZ_03960"
     CDS             complement(3690..4250)
                     /locus_tag="AC16UZ_03960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6245870.1"
                     /translation="MADETDTQATNYDDVRQYRLDPEREQELVNTAGECVFCWANKAG
                     HPIGVITAYVPVKGKIWMTATRERVRIKAIARDGRSSVVISSKGTPMGGGKTVTYKGH
                     TVIHDDQDTKMWMYNLLAQGMAASKDEGNEFTKGLDPEMFVRFLDTPERVVLEFTPEM
                     SIPFDGDKMAMGTAAAYAAFAKENQS"
     gene            4367..>4590
                     /locus_tag="AC16UZ_03965"
     CDS             4367..>4590
                     /locus_tag="AC16UZ_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245871.1"
                     /translation="MKPVRNKPSYREIIQGDATPVADVLALTANPQQSTEDIPFEHYI
                     SDEFFEREMRKMWPKVWQIACREEHIPEVG"
BASE COUNT          712 a         1495 c         1534 g          849 t
ORIGIN      
        1 ggcgcgccaa tcccctctac gagacctttc tccaggcggc tgagcaagcg gggcatgcgc
       61 tcagcgacga cctcaacggt taccggcagg aaggctttgg tgacctggag atgacggtgc
      121 gcgacggggt tcgctgcagc gcggccctgg cctatctcgc ccctgctgtc tcgcggagca
      181 acctgacggt ccgctcgggc tccccggttc agcggctgaa cgtggaatcg ggccgctgcg
      241 aaggcttgac ctaccggcat cggggggcaa cccacaccgc ggtggcccgg gaggaggtgg
      301 ttctggcggc aggagccatc ggctctcctc agctgctcat gctctccggc atcggacacg
      361 gggaagccct cgaacctcac gggattcccg tgcgtcacga gctccccggg gtgggtgcca
      421 acctcatgga ccatctggag atctattttc agcaggcgtg cactcagccc gtgtcgctca
      481 accgctggct caacccgttg ggcaaggggg tgatcggcgc tcggtggctg ctcaccgctt
      541 ccggtccggg ggcctccaac catttcgaga ccggcgggtt cgtgcgcagc gcagccgacg
      601 tcccctggcc ggacgtccag tttcactttc tgccggcggc cgtgtcctac gacgggcggt
      661 cggtggccga tgcggacggt tttcaggtgc acgtggggcc catgctgagc cccagccgcg
      721 gccgcctcac cctgcgctcg gcagacccgg acgtcgcccc cgtcatcagt ttcaactaca
      781 tgtcctgcga ggaagactgg caggtgttcc ggcgggcgat tcgcctctcc cgggagctct
      841 tcgcccagcg ggcattcgac ccttatcggg gtgtcgagat cgccccgggg agcggcgccg
      901 attcggacgg ggcaatagat gccttcgtgc gccgcagcac ggagagtgcc tatcacccct
      961 gcggaacctg ccgcatgggg aacgacccaa tggcggtggt ggactcggag tgccgcgttc
     1021 gcggaatcca ggggctgcgg gtcgtggacg cctcgatctt ccctcacatc accaacggca
     1081 acctcaacgc ccctacgatc atgaccgctg aaaaggcggc agacgccatt ctcgggcggc
     1141 gattggccgc ggaagcgctg ggttatttcg acgcgccggc cagccccacg cgatgatgac
     1201 gtctcgctgg cgtttgcgag tgctgggctt ggtgttgctc aacgggatag gcgtctgcag
     1261 catggcccag gcggatgaga agccggtgcc ccgggatgcg ggtctcgagt ttgtctacga
     1321 cgacggcttt acggaatcgg agcgccgcaa gctggaagcc tggattgccg aggtggccgg
     1381 tgcgctggag gttctcgtcg ggcctttgcc catggacgtg cgttttcgat ttgcccgtca
     1441 cgagggggcg ggccccgttc cctgggccaa caccctgcgc ggccgccgtc agggcgtgcg
     1501 cttgtatgtg gacccgacac agccggtcga ggcgtttcgc aatgactgga ccgcgcctca
     1561 cgagctcagc catctggttc tgcctttcgt cggccgcgag catgcctggt tttccgaggg
     1621 cttcgccagc ttcatgcagt tccgcgtcat gcggatcatg gacgtgctgg acgacgaggc
     1681 gatggctgcg cgctactcgg aacgtctcga cacggctgcc gccgcctaca gccagccgaa
     1741 ccgccggttc gtcgaggcgg ttccccagct gcgtgccgag gggctctacc cgaccattta
     1801 ctggggtggc gccagctact ttcttgagat ggaggagcgc ttgaagcgca agggcgcctc
     1861 gctcgtcggg gtgcttgcgg cctatctggc ctgttgccgg cgcaacgagg acgatctcga
     1921 ggacctggtg gccaggctcg atgacctggc gggctcgacg acgttctccg acgggctgac
     1981 cggctttcgc cgtgtcgtgg ggtttccacc ccaccggcat ctccaggcgc tcgctccccg
     2041 ttagccgctc tgcgtggagg cgggcgcgtt tccggtggtc cgccacacct cggtggtggc
     2101 cagatccttg gcgacgatgg attgcgccga aggccgcgca acgacgcgct gatagtgcgc
     2161 gtcgaaacgg ggccagcgcg tggcatccag cgggtcaccg gcgagatcga gcagccgcac
     2221 gggggcggcc agcgccatgt cggcgatgcc gaaccggccg cccaccatcc cgtcgcccgt
     2281 ctccggcgct gcccggtcca gatagtcgaa cacccccggg taagcctctt ccttgaaagc
     2341 gtcgatctcg ccctggtctg ccggcctgcc ggtacggaaa ggaatgatga tgtgcatcca
     2401 gaaaatccgc gcgcacacct ggttcagcgc cgtgtcggca tactcctcga gccacagcgc
     2461 ccgcgcgcgg tcgcggttgt tcgaggggta gagcgcgggc ccgggatggc accgctccag
     2521 gtagtcgcag atcacggacg agtcggcgat gaagttccca tcgggctcct ccagcaccgg
     2581 gatccggccc agagggttca tggctagaaa gcgttgggac ttgtccagag ggtctagctc
     2641 gcaatgctcg aactcgagcc ctttttcctt catgaagatc cacgtcttgc gcacgaacgg
     2701 tgagctggtg gcgccgtgaa gtctgtacat tgaaacgtcc tctcgggtcg gtcgtgctct
     2761 tcgggcgtca gccggtcagc agagccttca cctcggccag acgctcgatg gtggccaggc
     2821 tttcgtcctg ccgggcgctg aggttgcgca cgatgacgtc ggtgtatccc tgatcggcaa
     2881 aggacctcag ctgatcggcc acctcgccag gagagccgaa cagcagggcg tcctcgctca
     2941 tgccccgata gccggcctcc acgtaagggg tgacggtggc gcgcgcggcc tggctggtcg
     3001 cgccgacgaa tatgtctcgg cgcagggcga cggcggcagg cgatctgccg aactccgcgc
     3061 aggcctgccg gtagacgttt gccgcgtcag cggtctgggc gggggtgagg tttggcgagg
     3121 ccagccatcc ttcgccaagc tgggcggcgc gccgtatggc aggctgcacc agagcgccga
     3181 tccagacctc cacgggctcc ggcggcagcg gggcgatgcg ggcgccgcag aggtgccagt
     3241 agcgttcctc gtcgacgctc tcgccccgcc aaagggcgcg catgacttcg aggctcgcac
     3301 gaaacatgcc cacccgtcgg gacatgtcca cccccatggc cttgccctgc cggtcatcgc
     3361 ccagcccgca ctgcatgatg aagcgccccg gcgcgatgct cgccagggtg gcgatctgct
     3421 ctgccagcac gacggggtgc cacaggggca gtaggtagag cgcgccgaac ggcatctcgc
     3481 tccactcggc cagcatgcgt cccaggatcg gcgtgttctg atagtacgtg aacggcgtga
     3541 cgtggtggtc gccgacgaac agggagtcga gccccgcgtc cctggcggca cgggcgcgct
     3601 cgatcatgtt gcgcgcgccc tcgcggggat catcaacctg gtagctggaa gagaccgata
     3661 tgccgatgcg catcatgccg ccctcgccgt caggactggt tttccttggc aaacgccgcg
     3721 tacgccgccg ccgtgcccat ggccatcttg tcgccgtcga agggaatgga catctccggg
     3781 gtgaactcca gcacgacgcg ttcgggagta tccaggaagc gcacgaacat ctccgggtcc
     3841 agccccttgg tgaactcgtt gccctcgtct tttgacgctg ccatgccctg ggccaagagg
     3901 ttgtacatcc acatcttggt atcctggtcg tcgtggatga ccgtgtggcc tttgtaggta
     3961 accgtcttgc cgccacccat gggggtgccc ttgctcgaga tcaccaccga tgaccggccg
     4021 tctctggcga tggctttgat gcgcacgcgc tcgcgggtgg ccgtcatcca tatcttgcct
     4081 ttcacgggca cgtaggccgt gatcacgccg atggggtgac ccgctttgtt cgcccagcag
     4141 aagacgcact cgccggcggt gttgaccagc tcctgctcgc gttccgggtc cagccgatac
     4201 tgccggacgt cgtcgtagtt ggttgcctgg gtatcggttt catcggccat cgtggtgctc
     4261 cctttcaacg tgccttcgcc ccggtgtgtt cgacgcttga tgcgtggttg gccggcaagt
     4321 ataacggttt gcacatcggc tcgatgggcc gtatggggag gacatcatga agcctgtgcg
     4381 taacaagcca agttaccggg agatcattca aggcgatgcc acgcctgttg cggacgttct
     4441 cgcgctcacg gcgaacccgc agcagagcac agaagacatt ccgttcgagc attacatctc
     4501 agacgagttc ttcgaacgcg agatgcgcaa gatgtggccc aaagtctggc agatcgcctg
     4561 ccgcgaggag cacatccccg aggtgggcga
//
LOCUS       DBNHOX010000184         4202 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1364653, whole genome shotgun sequence.
ACCESSION   DBNHOX010000184 DBNHOX010000000
VERSION     DBNHOX010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1364653"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /gene="lpxK"
                     /locus_tag="AC16UZ_03970"
     CDS             <1..1188
                     /gene="lpxK"
                     /locus_tag="AC16UZ_03970"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017134187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - lipid-A 4'-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIR6245872.1"
                     /translation="RIVEAGTHQALLNRGGHYAALYQAQFDDEPDSAAVAEAPTGSTA
                     PGLTGKLTVGQTLRPLAQAWYTGSRWTVLLAPFAWAFERYAVRRRLRFLTGKRRSWRS
                     PVPVIVVGNITAGGTGKTPMVIWLVEQLRALGYSPGVVSRGYGGKRGRKPLLVTPDSD
                     PAVVGDEAPMIASRARCPVVVAADRARAVESLLQAANSDVVVSDDGLQHYALARDLEI
                     ALIDGHRGVGNGRCLPAGPLREPVSRLESVDWVISNGLGSGIVARETVMTLIPTAFVR
                     VTPGPGEARQRPEDFVAAHPNVNAVAGVGNPARFALALRELGLAPVLHAQPDHHPYDG
                     SELVFDNDWPVVCTEKDAVKIRRLTDLPADVWYLEVEAHLPDGAAAFLVELLRAHGIS
                     RHG"
     gene            1181..1945
                     /gene="kdsB"
                     /locus_tag="AC16UZ_03975"
     CDS             1181..1945
                     /gene="kdsB"
                     /locus_tag="AC16UZ_03975"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003317470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIR6245873.1"
                     /translation="MAEPFKVVVPARYGSSRLPGKPLADLHGKPMIVRVAERCAASGA
                     EEVVIATDDVRIVEAVTSAGFEATMTRADHASGSDRVMEVARLRAWDDDAIVINVQGD
                     EPQMPPAVVMQVAQILQADPARPVATLCEPIREVAVLTDPNVVKVVRDRWGRALYFSR
                     APIPYERDEAPGKSGQDRGRPIGLRHIGVYGYRLSALQQFVELPVSDLERLEKLEQLR
                     LLENGIPIFVTEACETVPAGVDTPADLERVRTRSPS"
     gene            complement(1953..2723)
                     /locus_tag="AC16UZ_03980"
     CDS             complement(1953..2723)
                     /locus_tag="AC16UZ_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6245874.1"
                     /translation="MDLNGKVAVITGGASGLGRATAEMVINNGGKVALFDLNEDLARQ
                     TAAALGDDAAPFAVDVTNDESAAAAVDQVLERFGAIHVNVNCAGIGAAGRTISKEGAM
                     PLKHFNFIIQVNLVGTFNTLRLCAAAMNENEPTTEDGEKGVIINTASVAAFDGQIGQA
                     AYSASKAGVAGMTLPIARDLARSGIRVCTIAPGIFETPMMAGAPDRVRDPLIEMTQFP
                     KRLGNAAEYALLARQIIENTYLNGETIRLDGGIRMSPR"
     gene            2695..3303
                     /locus_tag="AC16UZ_03985"
     CDS             2695..3303
                     /locus_tag="AC16UZ_03985"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF024922.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB
                     family protein"
                     /protein_id="HIR6245875.1"
                     /translation="MTATLPFRSTVLPSTNERASIPQAGYRHFPSYLADTPADVLLGW
                     LRESVDWRSERIRILGKSVAVPRLVAWYGDPGVCYRYSGVEHVAGGWPSQLCSVRDLL
                     RREWSAGFNFVLINRYRDGSDSMGWHRDDEPELAGPIASLSLGATRRLRVRVEGGTGS
                     IALDLSHGALLCHERHLPHALMKTRRPVGERINLSFRTVVSR"
     gene            3300..>4202
                     /gene="murB"
                     /locus_tag="AC16UZ_03990"
     CDS             3300..>4202
                     /gene="murB"
                     /locus_tag="AC16UZ_03990"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR6245876.1"
                     /translation="MIAAPRLDALNTLGVAATADRLVVVESERALQGLLREAADQRGG
                     LVVLGGGSNVVLKRHVPGTVAVIRTRGTHIVPGRGGRVNVSVAAGESWHGLVRFTIGQ
                     GLYGLENLALIPGSVGAAPLQNSGAYGVELADRLISLRAMEVASGAVREFLREECGFA
                     YRDSRFKSADAGRFIILDVTLALSREAQPVIDYPELRLELQRLGGSRISPVLVAEAVV
                     RVRRRKLPDPRRVGNAGSFFKNPVVSAPKAEGLRAAVPGLVEHFQSDGRVKLSAAQLI
                     DRAGFKARAAGKVGVWPRQPLVLIN"
BASE COUNT          665 a         1326 c         1431 g          780 t
ORIGIN      
        1 aggatcgtag aggcgggtac tcaccaggcg ctgctgaacc ggggtggaca ttacgccgcg
       61 ctctatcagg cccagttcga cgacgagccg gattccgctg cggtggccga ggcacccacc
      121 gggtccacgg ctccgggcct gaccggcaag ctgacggtgg gccagaccct gcgccccctg
      181 gcccaggcct ggtacacggg cagccgctgg acggtgctgc tcgcgccttt cgcatgggcg
      241 ttcgaacgct acgcggtgcg tcgcaggttg cgctttctta ccggcaagcg ccgctcctgg
      301 cgcagcccgg ttcccgtgat cgtggtggga aacatcaccg cgggtggtac gggcaagacg
      361 cccatggtta tctggttggt cgagcagttg agggcgctcg ggtactcgcc gggagtcgtc
      421 tccagaggtt acggcggtaa acggggacgc aagcccctgc tggtgacccc ggattcggac
      481 cccgccgtcg tcggtgacga agcgcccatg atcgcgagcc gggcccgctg cccggtggtg
      541 gtggcagccg atcgtgccag ggcggtagag agtcttctgc aggcggcaaa cagcgacgtg
      601 gtcgtctcgg acgacgggct gcagcactac gccctggcga gggatctcga aatcgcgctg
      661 atcgatggtc atcgaggcgt cggcaatggc cgctgccttc ccgccgggcc gctgcgcgag
      721 cccgtcagcc ggctcgagag cgtcgactgg gtgatcagca atggcctggg atccgggatc
      781 gtggcccgag agaccgtcat gacgctgatc cccaccgctt tcgttcgagt cacgccggga
      841 ccgggagagg cacgccagcg tccggaggat ttcgtggccg ctcatccgaa cgtcaacgcc
      901 gtcgccggtg tgggcaatcc cgccagattt gcgctggctt tgcgcgagct ggggcttgcc
      961 ccggttcttc atgcccagcc cgatcatcat ccctacgacg gcagcgagct agtcttcgac
     1021 aacgactggc cggtggtctg caccgaaaag gatgcggtga agattcggcg cctgaccgac
     1081 ctgccggccg atgtctggta cctcgaagtg gaggcgcatc tgcccgacgg cgccgctgcg
     1141 ttcttagtgg agctgttgag agcccacggg atcagccgcc atggctgagc cgttcaaggt
     1201 cgtggtgccc gcccggtacg gctcgtccag acttcccgga aagcctctgg cagatctgca
     1261 cggcaagccg atgatcgtgc gggtggccga acgctgcgcg gcctccggcg cggaggaagt
     1321 ggtgattgcc acggacgatg tccgaatcgt ggaagcggtg acttccgcgg gtttcgaagc
     1381 gaccatgacc cgcgccgatc acgcgtcggg ttccgaccgg gtcatggagg tcgcccgctt
     1441 gcgggcctgg gacgacgatg ccatcgtgat caacgttcag ggtgacgagc cccagatgcc
     1501 acccgccgtc gtcatgcagg tcgcgcagat ccttcaggca gacccggccc gtccggtggc
     1561 cacgctgtgc gagccgatca gggaggtggc ggtcttgacc gatcccaacg tcgtcaaggt
     1621 ggtgcgggac cggtgggggc gagcgctgta cttttcccgc gccccgatac cttacgagcg
     1681 tgacgaagcg ccagggaaat ccggacagga tcgagggcgt ccgatcggcc tgcgccacat
     1741 cggcgtctac ggatatcgcc tctcagccct gcagcagttt gtcgagctgc ccgtgtcaga
     1801 cctcgagcgc ttggagaagc tggagcagct gcgcctgctg gaaaacggca ttccgatctt
     1861 cgtcacagag gcctgcgaaa cggttccggc cggcgtggac acgccggcgg acctggaaag
     1921 agtccgaacc cgatccccat cgtgaggcgc tcttagcggg gcgacatccg gattcctccg
     1981 tccagccgaa tggtttcgcc gttcaagtag gtgttctcga tgatctgccg ggcgagcaat
     2041 gcgtactccg ccgcgttgcc gaggcgtttt ggaaactgag tcatctcgat gagcggatcc
     2101 ctgactcggt ccggtgcgcc agccatcatc ggcgtttcga agatgccggg ggcgatggtg
     2161 cagacccgga taccggatct ggccaggtcc ctggcgatgg gtaaggtcat gccggcgacg
     2221 ccggccttgc tcgcggagta cgctgcctga ccgatctggc catcgaacgc tgccaccgag
     2281 gcggtattga tgatgacgcc tttctcgccg tcctccgtgg tcggctcgtt ctcgttcatc
     2341 gcggccgcgc aaagcctcag ggtgttgaac gtgccgacga gattcacctg aatgatgaaa
     2401 ttgaagtgct tcaagggcat cgcaccttct ttggagatgg tccggcccgc agcaccgatg
     2461 cctgcgcagt tcacgttcac gtgaatcgcg ccgaatcttt ccagaacctg gtcgacggcc
     2521 gcagcggcgg attcgtcgtt cgtcacgtcg acggcaaagg gtgcagcgtc gtcgccgagc
     2581 gcggcggcgg tctgacgggc aaggtcctcg ttgaggtcga acaaggcgac tttgccgcca
     2641 ttgttgatta ccatctccgc cgtcgcgcgc ccgaggccgg atgccccacc ggtgatgaca
     2701 gccactttgc cgttcagatc cactgttctt ccctccacaa acgagcgcgc gagtataccc
     2761 caagcaggct atcgccattt cccgtcatac ctcgctgata ccccggcaga cgtcctgctc
     2821 ggctggctgc gcgaaagcgt cgactggcga agcgagcgga tcagaatact cgggaagtca
     2881 gtggcggtgc caaggctcgt tgcctggtac ggagaccccg gggtctgcta ccggtattcg
     2941 ggcgttgaac acgttgccgg gggctggccc tctcagcttt gctcggtgcg cgatctcctg
     3001 cggcgcgaat ggtcggcggg cttcaacttc gtgctgatca atcgatatcg cgacggctcc
     3061 gactccatgg gttggcaccg ggatgacgag cccgagctgg cagggccgat cgcgtctctg
     3121 agcctcggcg cgacccgtcg gctgcgagtc cgggtggagg gcgggaccgg aagcattgcg
     3181 ctggacttga gccacggggc gcttctttgc cacgaacggc accttcctca cgccctgatg
     3241 aagacacgcc ggcccgtggg agaacggatc aacctgtcgt ttcgcacggt cgtctcccgg
     3301 tgatcgcggc gcctcgactg gacgcactca ataccctggg cgtcgctgcg acggccgatc
     3361 ggttggtcgt ggtcgagagc gagcgggcgc tgcaggggct cctgcgtgaa gccgccgatc
     3421 agcggggcgg gctcgtcgtg ctcggcggcg gcagcaacgt cgttctcaag cgccacgttc
     3481 cgggaaccgt cgccgtcatt cgtacccgtg gtacgcacat cgtccctggc cggggagggc
     3541 gggtgaacgt ctccgtagcg gccggggaga gctggcatgg gctcgtgcgt tttactattg
     3601 gccagggcct gtacgggctg gaaaaccttg ccctgatacc agggtccgta ggggctgccc
     3661 cattgcagaa cagtggcgcc tacggcgtgg aactggccga tcggctgatc agcttgcggg
     3721 ccatggaagt cgcctcgggc gccgttcgag agtttcttcg ggaagagtgc ggtttcgcct
     3781 atcgcgacag ccgcttcaaa tctgctgacg ccggtcgttt catcattctg gacgtgaccc
     3841 tcgcgctttc gcgtgaggcg cagccggtca tcgactatcc ggagctgcgc ctcgagctgc
     3901 agcgtctggg cggctcacgg atctcgcccg tgctggtcgc cgaagcggtt gtgcgtgttc
     3961 gtcgccgcaa gctgcctgac cctcgccggg tagggaatgc cggcagcttc ttcaagaatc
     4021 cggtggtttc cgcgcccaag gccgaaggtc tacgcgctgc agtgccgggc ctggtcgagc
     4081 atttccagag cgacggcagg gtgaagctat ccgcagcgca gctgattgat cgggccggtt
     4141 tcaaggcgcg ggccgccggc aaagtcggcg tctggccgcg ccagccgctc gttctgatca
     4201 at
//
LOCUS       DBNHOX010000185         6253 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_722326, whole genome shotgun sequence.
ACCESSION   DBNHOX010000185 DBNHOX010000000
VERSION     DBNHOX010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6253)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_722326"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /gene="alr"
                     /locus_tag="AC16UZ_03995"
     CDS             <1..1067
                     /gene="alr"
                     /locus_tag="AC16UZ_03995"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR6245877.1"
                     /translation="QHAALIVRGRLHVDLAALAANYRSFSSAAEAAGGTAGGVVKADA
                     YGLGAARVAATLQSEGCAEFFVATVEEGAALRAILPEAIIYVFEGVTGATAEVLASND
                     LVPVLNHRGQLEAWQAYRERPAAVHVDTGMNRLGFPADVDAVQFAGMNLSLLLTHLAC
                     ADVPDDPMNHEQVERFRRAATSFPGVRTSIGNSAGMLADASCCGDLGRPGVGLYGGNP
                     FSDRRNPMRRVAALQGQVLQVRTCSAGSPLGYGATYVAPADLRVAVVGLGYADGLPRL
                     ISGRGCAAVGGRRCALLGRVSMDLTLVDVTGLEVQAGDWIEMFGDTVSVDEVAGWAET
                     IAYEILTGIGDRVERVYSGD"
     gene            complement(1052..1663)
                     /locus_tag="AC16UZ_04000"
     CDS             complement(1052..1663)
                     /locus_tag="AC16UZ_04000"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6245878.1"
                     /translation="MAETAVDAGVERAGKAGGAPGSIQSAAELLVADEAALGCHREVT
                     AADPNPEPCDVFLRLTADSLDETLRQAAAHNNRGLILTRLGGLEAALADFEAALALAP
                     GLGAAEVNRGTALLHMGLYPDALKAFENVIVNDEEHRATARFNRSFVYRALGELDLAA
                     ADLASAAQTDLPVSRSREPIPLQAGPAAEPLDRPMELEGQSPE"
     gene            1858..3243
                     /locus_tag="AC16UZ_04005"
     CDS             1858..3243
                     /locus_tag="AC16UZ_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HIR6245879.1"
                     /translation="MADPLHNQFPPGSLEARDLEHLLHPTTNLEQHHKVGPRVHDRAE
                     GVYLWDSQGRQYLEGMAGLWCTALGYGEEELVSAAAQQMRKLSYSQLFGGRSNEPSIL
                     LAERLKAMAPFDAGRVFFGLSGSDANDTQIKLMWYYHNAIGKPEKKKIISRMRGYHGV
                     TVAAGSLTGLPAFHAHFDLPIPGVLHVDAPHYYREAKGGETETEFVDRLVGDLEELIL
                     GEGPETIAAFIAEPVQAAGGVIVPPPGYFAKVQALLARHDILFIDDEVICGFGRTGRA
                     FGAQTFGIEPTTMSVAKGVSSAYLPLSAVIIPEFMYEPLVELSGSVGTFGHGFTYSGH
                     PVCAAVALRTLELMEEREIFAHAAAVSEPFQERLALLADHPLVGDARGVGLLGAVELV
                     ADKDTRTPFPPGQGVGAHCQERCESHGLILRALGDVVAICPPLIISEDQVDELFAKLE
                     RALDDTFAWVH"
     gene            3334..4944
                     /locus_tag="AC16UZ_04010"
     CDS             3334..4944
                     /locus_tag="AC16UZ_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017709070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR6245880.1"
                     /translation="MAILQSNIDTGAAAFAANAKHHRELADELRTLAGRIVEGGDARS
                     RERHVGRGKLLPRDRLSRLLDPGSPFLEVGQLGGYELYDDWIPSGGIVTGIGRVSGTQ
                     CMVVVNDATVKGGTYYPITVKKHLRAQEIALENGLPCVYLVDSGGAFLPMQAEVFPDR
                     DHFGRIFYNQANLSARGIPQIAVVMGSCTAGGAYVPAMCDQAIIVRGQGTIFLGGPPL
                     VKAATGEEVDAESLGGGDVHSRTSGVTDYLAADDVHALGIAREIVAASQQRYRATVET
                     RTPRPPAFAEEELYGVLPTDPRTPVDVREIIARLVDGSEFHEFKALYGETLVCGFAHI
                     EGMPVAVIANNGILFSESALKGAHFIQLADRRGVPLLFLQNITGFMVGKKYENQGIAK
                     DGAKMVTAVACARVPKFTVVIGGSFGAGNYAMCGRAYGARMLWTWPNARISVMGGEQA
                     AHVLATVRRDGAAARGEEWPEAEQAAFMDDIRAQYETQGHPYFASARLWDDGVVDPAD
                     TRRVLGLALEVACREPLQPQSGFGVFRM"
     gene            4947..>6253
                     /locus_tag="AC16UZ_04015"
     CDS             4947..>6253
                     /locus_tag="AC16UZ_04015"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR6245881.1"
                     /translation="MFDTLLIANRGEIACRVMRTATRMGLRTVAVYSDADAHAQHVRL
                     ADAAERIGPAPARESYLRIDAVLDAAERHGAGAVHPGYGFLSENAEFAAACEERGIVF
                     VGPSARAIRAMGSKIEAKRIVAAAGTPVVPGYQEADQSDAALSRAAAEIGYPLLIKAS
                     AGGGGKGMRLVREPDEFPAALAGARREAAAAFADELVLLERYLDAPKHLEVQILADQT
                     GHTLHLYERDCSVQRRHQKVIEEAPGPTVSEELRTLLGEAAVQAAKAIGYVGAGTVEF
                     ISQAGEFYFMEMNTRLQVEHPVTEAITGLDLVEWQLRVAAGEKLPFRQHEIPRNGHAV
                     EARVYAENPARRFLPSTGTLAHVAFPQEVRVDAGVATGDAVSVHYDPMLAKVIAHGPD
                     RDAAITSLERALARTEIAGVAHNVAYLRRLLAHPVFRGGDYTTG"
BASE COUNT          977 a         2005 c         2179 g         1092 t
ORIGIN      
        1 atcaacacgc tgcgctgatc gtgcgcggcc ggctgcacgt cgacctggct gcgctcgccg
       61 caaattaccg gtcgttctcc agcgcggctg aggccgcagg cggaaccgcg ggtggggtcg
      121 tcaaggccga cgcctacggt ctgggcgccg cgagggtggc ggcaactctg cagtctgagg
      181 gttgcgcgga gttcttcgta gccaccgtgg aggaaggcgc cgcgctgcgg gccatcctgc
      241 cggaagccat catttacgtt ttcgaaggcg tcacgggggc aaccgcggaa gtgctggcat
      301 ccaacgacct cgttcccgtg ctcaaccatc ggggtcagct cgaagcctgg caggcttacc
      361 gggagcgtcc tgccgccgtc catgtggata ccggtatgaa ccggctgggg ttccccgcgg
      421 acgtcgatgc ggtgcagttt gcggggatga acttaagcct tctgctcacc catctggcct
      481 gcgcggacgt gcccgatgac cccatgaatc acgagcaggt cgagcgcttc cggcgcgccg
      541 ccacgtcgtt tccaggtgta cgcacgagca tcggcaactc cgcgggcatg ttggccgacg
      601 cgtcgtgctg cggtgacctt ggccgtcccg gtgtcgggct ctacggcggc aatcccttct
      661 ccgaccgacg caaccccatg cgccgggtgg ccgccctcca agggcaggtc ctgcaggtga
      721 ggacgtgctc ggctggcagc ccgctgggct atggggcgac ctacgttgcg cccgccgatc
      781 tgcgggtggc cgtggtgggg ctgggttacg cggacggcct gccgaggctg atatcgggcc
      841 gaggctgcgc tgccgtgggt ggtcggcgct gcgctttgct cggccgggtt tccatggacc
      901 tgaccctggt ggacgtaacc gggctcgagg ttcaggccgg cgactggatc gagatgtttg
      961 gcgacacggt gtccgtcgac gaggttgccg gctgggcgga gaccatcgct tacgagattc
     1021 tcaccggcat cggcgatcgc gtggagcgcg tctactccgg ggattgaccc tcgagctcca
     1081 ttggccggtc caggggctct gcggcgggtc ccgcttgcag gggtatcggc tcgcggcttc
     1141 ggctcacggg caggtccgtc tgcgcggcag atgccaggtc ggcggcggcg aggtccagct
     1201 cgcccagggc ccgatacacg aaggagcggt tgaaccgtgc cgttgcgcga tgctcctcat
     1261 cgttgacgat gacgttctcg aacgctttga gcgcgtcggg gtagagcccc atgtgaagga
     1321 gcgcggttcc gcggttgacc tctgccgcgc ccaacccagg ggccagagcg agagccgctt
     1381 cgaagtcggc tagcgccgcc tccaaccctc ccagcctggt caggatcaga cctcggttgt
     1441 tgtgcgccgc tgcctgccgg agagtctcgt ccaagctgtc ggcggtcagt ctcagaaaaa
     1501 cgtcgcaggg ctcgggattg gggtctgctg ccgtgacttc gcgatggcag ccgagtgccg
     1561 cttcgtccgc cacgagcagc tcggccgccg actgtatcga gccgggcgcg ccgccagctt
     1621 tcccggctcg ctccacgccc gcgtccacag cggtctcggc caacgcagca cctgccgtca
     1681 gagcaatcag catcgagaag gtgataatcg cggttcgggc gagcatgagg cgcagtgtaa
     1741 cgcaatggcg gcaggcgcag cgcggacgca gcgcgggcgc tgcgaccgcg ctgctttgcc
     1801 gtagcgctcg gacccgccgc cccgtaagct tcgccttccg cctgtcggga gacgttcatg
     1861 gccgatccgc tgcacaatca gtttccgccc ggctccctgg aggccaggga tctggagcac
     1921 cttctccacc ccacgacgaa tctggaacag catcacaagg tgggtcctcg tgtccacgac
     1981 cgggccgaag gcgtctatct ctgggattcc cagggccgtc agtatctcga aggcatggcg
     2041 ggcctttggt gcacggcgct cggctacggg gaggaagagc tcgtcagcgc ggccgcgcag
     2101 cagatgcgca agctcagcta ctcgcagctg ttcggcggcc gcagcaacga gcccagcata
     2161 ctcctggccg agcggttgaa agccatggcc cccttcgatg ccggtagagt cttcttcggt
     2221 ctctcgggca gcgatgccaa cgacacccag atcaagctca tgtggtacta ccacaatgcc
     2281 atcggcaagc cggaaaagaa gaagatcatc agccgcatgc gcggctatca cggcgtgacg
     2341 gtggccgccg ggtccctgac cggtcttccc gccttccacg cgcacttcga tctgccgatc
     2401 ccgggggtgc tgcacgtcga tgctccacac tactatcgcg aagcgaaggg cggcgagacc
     2461 gagaccgagt tcgtcgaccg gctggtcggc gatctggagg agctgattct tggcgagggc
     2521 cctgaaacca ttgccgcgtt catcgcggaa cccgttcagg ccgccggcgg ggtgatcgtg
     2581 ccgccgcccg gctacttcgc caaggtgcag gcgctgttgg cccgtcatga catcctcttc
     2641 atagacgacg aggtcatctg cggcttcggc cgcacgggcc gcgcgttcgg tgcgcagacg
     2701 ttcgggatcg agccgaccac catgagcgtg gcgaagggcg tctcttcggc ctacctgccg
     2761 ctaagcgccg tgatcattcc cgagttcatg tacgaaccgc tggtggagct tagcgggagc
     2821 gtgggaacct tcggccacgg ctttacctat tcgggccatc cggtgtgtgc ggcggttgcg
     2881 cttcggacgc tagagctcat ggaggagcgc gagatctttg ctcacgcagc agccgtgtcg
     2941 gagccgttcc aggaacgtct ggcgttgctg gccgaccacc cactggtggg ggacgcgcgc
     3001 ggggtcgggc tccttggcgc ggtggagctc gtggcggaca aggacacccg cacgcccttt
     3061 cctcccgggc agggtgtggg cgcgcattgc caggaacgtt gcgagtccca cgggttgatc
     3121 ctccgagcgc tcggagacgt ggtggccatc tgcccgccgc tgatcatatc cgaggatcag
     3181 gtcgacgagc tcttcgcgaa actggaaagg gcgctggacg acacgttcgc ctgggttcac
     3241 tgaacagcga gcgttcgcgt cacagctgcg gcgccttggc aggcagttca ttgggttgac
     3301 gtcgttgtgt atctttgccg tctgggggga gttatggcca ttctgcaatc gaatatcgat
     3361 accggcgccg ccgcgttcgc cgccaacgcg aagcaccacc gcgagcttgc cgatgagttg
     3421 aggacgcttg ccgggcgcat cgtcgagggc ggggacgccc gctccaggga gcgtcacgtt
     3481 ggtcgcggca agcttctgcc gcgggaccgc ctcagccgtc tgctggatcc gggctcacca
     3541 tttctggagg ttggccagct tggcggctac gagctttacg acgactggat tcccagcggc
     3601 ggcatcgtca ccggcatcgg cagggtctcg ggcacgcagt gcatggtggt ggtgaatgac
     3661 gccaccgtca aaggcggaac gtattacccg attaccgtca agaagcactt gagggcgcag
     3721 gagatcgcgc tggagaatgg cttgccgtgc gtctatctgg tggattccgg cggcgcgttc
     3781 ctgcccatgc aggcggaggt ctttcccgac cgggatcact tcggtcggat tttctacaac
     3841 caggccaatc tctctgctcg cggcatcccc cagatcgcgg tggtgatggg atcctgcacg
     3901 gcggggggcg cttacgtgcc ggccatgtgc gaccaggcca tcatcgtccg gggccagggg
     3961 acgatctttc tcggcgggcc gcccctggta aaggccgcga ccggcgagga ggtggatgcg
     4021 gaaagccttg gcggcggtga cgtgcacagc cgcacgtcgg gcgtgaccga ctatctggcg
     4081 gcggacgatg tccatgccct gggcatagcg cgcgaaatcg tcgcggccag ccagcagcgc
     4141 tatcgcgcga cggtcgagac ccgcacgccc cgacctccgg cctttgccga ggaggagctc
     4201 tacggggtgc tgcccacgga tccgcgcacc ccggtggacg tgcgcgagat catcgcccgg
     4261 ctggtggacg gatcggaatt tcacgagttc aaagcgctgt atggagaaac gctggtctgc
     4321 gggtttgccc acatcgaggg gatgccggtt gccgtgatcg ccaacaacgg catcctgttc
     4381 tccgagtcgg cgctcaaggg cgcgcacttc attcagctcg ccgaccggcg cggggtgcct
     4441 ctgctctttc tgcagaacat caccggcttc atggtgggca agaagtacga gaatcagggc
     4501 attgcgaaag acggggcgaa gatggtcacg gcggtggcct gcgcgcgggt accgaaattc
     4561 accgtcgtga tcggtggctc tttcggtgcc ggcaactacg ccatgtgcgg gcgcgcctac
     4621 ggcgcgcgca tgctctggac gtggccgaac gcccggatat ccgtcatggg gggagagcag
     4681 gcggcccacg tgctggccac cgtacgccgg gacggggcag ccgctcgcgg ggaagagtgg
     4741 ccggaggctg agcaggcggc gttcatggac gacatccgtg ctcagtacga aactcaggga
     4801 catccctact tcgcgagcgc caggctctgg gacgacggcg tcgtcgaccc ggctgatacc
     4861 cggcgcgtgc tgggcctcgc gctcgaggtt gcctgccggg aaccgctgca gcctcagtcc
     4921 ggcttcggcg tcttcaggat gtagccatgt tcgatactct gctgatagca aatcgtggcg
     4981 agatcgcctg cagggtgatg cgcaccgcca cgcgcatggg cttacgcacg gtcgccgtgt
     5041 actccgatgc cgacgcccac gcgcagcacg tgcgtctggc ggacgcggcc gagcggatcg
     5101 gcccggcacc ggcccgcgag agctacctgc gcatcgacgc ggtgctcgac gccgcggagc
     5161 gccatggggc gggcgccgtt caccccggct acgggtttct ctccgagaac gcggagtttg
     5221 ccgctgcctg cgaggagcgc ggcatcgttt tcgtgggccc ttcggcccgc gccattcgcg
     5281 ccatgggctc gaagatcgaa gcgaaacgca tcgtggccgc ggcgggaacg cccgtcgtgc
     5341 cgggctatca ggaagcggat cagagcgatg cggcgctgtc ccgcgcggcg gccgagatcg
     5401 gttacccgct gctcatcaag gcctcggcag ggggcggcgg caaggggatg cgtctggtgc
     5461 gggaacccga tgagttcccg gctgccctgg ccggtgcccg gcgcgaagcg gcggcggcct
     5521 ttgcagacga gctcgtgctg ctcgagcgct acctggatgc gccgaagcac ctcgaggtgc
     5581 agatcctcgc ggatcaaacc ggccacacgc tccacctcta cgagcgcgac tgctcggtgc
     5641 agaggcgcca ccagaaagtg atcgaggagg cgcccgggcc gacggtttcg gaggagctgc
     5701 gtaccctgct cggggaggca gccgtgcagg cggccaaagc catcggctac gtcggagcag
     5761 gcaccgtgga gttcatctcg caggcgggcg agttctactt catggagatg aacacgcggc
     5821 tccaggtcga gcatccggtc acggaagcga tcaccgggct ggacctcgtc gagtggcagc
     5881 tgcgggttgc cgcgggcgaa aagctgcctt tccgacagca tgagataccg cgtaacggcc
     5941 acgcggtgga agcgcgcgtt tacgcggaga atccggcccg gcgttttctt ccttcgacgg
     6001 gaacccttgc ccatgtggct tttccgcaag aggtgcgtgt cgatgcgggg gtggccaccg
     6061 gggacgctgt gagcgttcac tacgacccca tgcttgcgaa ggtgatcgcc cacgggcccg
     6121 atcgtgacgc cgcgatcacc tccctcgagc gggccctggc gcgcaccgag attgccggcg
     6181 tggcgcacaa cgtcgcctac ctgcggcggc tcctggccca tccggtcttt cggggcggcg
     6241 actacacgac ggg
//
LOCUS       DBNHOX010000186         2721 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_208221, whole genome shotgun sequence.
ACCESSION   DBNHOX010000186 DBNHOX010000000
VERSION     DBNHOX010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_208221"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /gene="glyA"
                     /locus_tag="AC16UZ_04020"
     CDS             complement(<1..347)
                     /gene="glyA"
                     /locus_tag="AC16UZ_04020"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251134.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR6245882.1"
                     /translation="MFGKDQQIAGFDDEVAAAINEETRRQEEHIELIASENYASPRVM
                     EAQGGVLTNKYAEGYPRKRYYGGCEWVDKVEELAIERVKALFGAHYANVQPHSGSQAN
                     AAAYLALLEPGDT"
     gene            494..2176
                     /gene="ettA"
                     /locus_tag="AC16UZ_04025"
     CDS             494..2176
                     /gene="ettA"
                     /locus_tag="AC16UZ_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HIR6245883.1"
                     /translation="MAQYVYTMQGVSKTVPPKRTILENISLSFFPGAKIGVLGLNGAG
                     KSSLLRIMAGVDTEFEGEARPQKDIRVGFLPQEPKLDDSLDVRGNVEQGMGEIMSDLA
                     EYNEISDKFAEPMSDDEMAELLDRQGELATRIDAANGWDVDRTLEIAADALRLPPWDA
                     KVAHLSGGERRRVALCTLLLSKPDMLLLDEPTNHLDAESVAWLERFLAEYPGTVVAVT
                     HDRYFLDNVAGWILELDRGRGIPFEGNYSAWLEAKEKRLEMEASKEKARQRAIHSELE
                     WVRSNPKARQAKSKARLQRFEELMSKETQSRNETAELYIPPGERLGEKVIELSHVSKG
                     FDDRLLIDDLSVSIPQGAIVGIIGGNGVGKSTFFRMLTGQEAPDGGSIELGDTVELAY
                     VDQSRDALADQKTVWEEIADSQDVIRVGSYEIQSRSYVSRFNFKGPDQQKRVGDLSGG
                     ERNRVHLAKLLRRGANVLLLDEPTNDLDVETLRALEEALLNFPGTVLVISHDRWFLDR
                     VATHILAFEGDSHVEWFAGNFSEYETDHRRRLAEQGLDTEPRRVRYKPLSRG"
     gene            complement(2177..>2721)
                     /locus_tag="AC16UZ_04030"
     CDS             complement(2177..>2721)
                     /locus_tag="AC16UZ_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HIR6245884.1"
                     /translation="ANPSAIFLQRGELDSPGSVATVTWEGTRPLLVEIQALVDEVQTG
                     YAKRVAVGLDQQRLAMHLAVLHRHCGITLGDQDVFANVVGGVRIGETGADLAALLAVL
                     SSYRNRVLPKNLIVFGELGLTGEVRAVANGQQRLSEARKHGFSQAIVPFANRPKEAIS
                     GMAVHGVKSLRAAMDVIDTI"
BASE COUNT          489 a          884 c          898 g          450 t
ORIGIN      
        1 atggtgtcgc cgggttcgag cagggcaagg taggcggccg cgttggcctg agatccggag
       61 tggggctgga cgttggcgta gtgcgcgccg aacagcgctt tcacccgctc gatggccagc
      121 tcttccacct tgtctaccca ctcgcagccg ccgtagtaac gcttgcgggg atagccttcg
      181 gcgtacttgt tggtcagcac gccgccctgg gcctccatga cccgggggct tgcgtagttc
      241 tccgaggcga tgagctcgat gtgctcttcc tggcgtcgcg tctcttcgtt gatggctgcg
      301 gcaacttcgt cgtcaaaccc ggcaatttgc tgatctttgc caaacatggg cgcccccggt
      361 cgaaaacgcg caagtttacc ccagctcggc gccatctcgt acgcaatcct ttcatgggga
      421 aattgaattg aaccaccgcg gcgatttcgg tagcttgcgc gctctctgag tccatcgacc
      481 aggagcggca tccttggccc agtacgtcta caccatgcag ggcgtctcga agacggtccc
      541 acccaagagg acgatcctcg agaacatctc cctttcgttc tttcccgggg cgaagatcgg
      601 cgtgctcggc ctcaacgggg ccggcaagtc gagcctgctc aggatcatgg ccggcgtcga
      661 caccgagttc gagggcgagg ctcgccccca gaaggacatc cgggtggggt ttcttcccca
      721 ggagccgaaa ctcgacgatt ccctggacgt gcgcggcaac gtcgagcagg gcatgggcga
      781 gatcatgtcg gacctcgccg agtacaacga gatctccgac aagttcgccg agcccatgtc
      841 cgacgacgag atggcggagc tgctggatcg tcagggtgag ctcgccacca ggatcgacgc
      901 ggccaacggc tgggacgtgg accggaccct ggagatcgcc gcggacgccc tgcgcctgcc
      961 gccgtgggat gccaaagttg cccacctctc cggcggtgag cggcggcggg tggctctgtg
     1021 caccctgctg ctctccaagc cggacatgct cctgctggac gagccgacca atcacctcga
     1081 cgccgaaagc gtggcctggc tggagcgctt cctcgccgag tacccgggga ccgtggtcgc
     1141 cgtcacccac gaccgctatt tcctagacaa cgtcgccggc tggatcctgg agctcgatcg
     1201 cggccgcggc atcccgttcg agggcaacta cagcgcctgg ctggaagcca aggagaaacg
     1261 gctggagatg gaagcgtcca aggagaaagc ccgccagcgg gcgatccact ctgagctaga
     1321 gtgggtgagg tcgaacccca aggcccggca ggccaagagc aaggctcgcc tgcagcgctt
     1381 cgaggagctc atgtccaagg agacccagag ccgcaacgaa accgccgagc tctacatccc
     1441 gccaggggag cggctggggg aaaaggtcat cgagctgtcc cacgtgagca agggcttcga
     1501 cgaccggctg ctcatcgacg atctgagcgt atccatccct cagggcgcca tcgtcggcat
     1561 catcggcggc aacggggtgg gcaagtcgac cttctttcgt atgctgaccg ggcaggaggc
     1621 gccggacggc gggagcatcg agctgggcga caccgtggag ctggcctacg tggaccagag
     1681 ccgggacgcc ctggccgacc agaagacggt atgggaggag atagccgaca gtcaggacgt
     1741 catccgcgtg ggaagctatg agatccagtc gcgcagctac gtctcccggt tcaacttcaa
     1801 aggtccggac cagcagaagc gggtcggcga tctctcgggc ggcgagcgca accgcgtgca
     1861 tctggccaag ctgctgcgcc gcggcgcgaa cgtgctgctg ctggacgagc caaccaacga
     1921 cctggacgtg gagaccctgc gcgcgctcga ggaagcgttg ctgaactttc cgggaaccgt
     1981 cctggtgata tcccacgacc gctggttcct ggaccgtgtg gccacccaca tcctggcttt
     2041 cgaaggagac tcgcacgtgg agtggtttgc ggggaacttc tcggagtacg aaaccgacca
     2101 ccgcaggcgg ctcgccgagc aggggctgga tacggagccc cgccgcgtgc gctacaagcc
     2161 gctttcccgc ggctgatcag atcgtatcga tcacgtccat ggcggcacgc aggctcttga
     2221 ccccgtggac ggccatgccg gaaatggcct ccttgggccg gttcgcaaac ggcacgatcg
     2281 cctggctgaa gccgtgcttg cgggcttcgg acagacgctg ctgcccgttg gccaccgcac
     2341 gcacctcgcc ggtcaggccg agctctccga acacgatgag gttcttgggc agcacacgat
     2401 tgcggtagga cgacagcacc gccagcaacg ccgccagatc cgcgcccgtc tccccgatgc
     2461 gcacgccgcc gaccacgttg gcaaagacat cctgatcgcc aagagtgatg ccgcagtgcc
     2521 ggtgcagcac ggcgaggtgc atggccagcc gctgctggtc caggcccacg gcgacccgct
     2581 tggcgtagcc ggtctgcacc tcgtccacga gagcctggat ctccacgagc agtgggcgcg
     2641 tgccttccca ggtgaccgtg gcgacgctgc cgggcgagtc gagctcgccg cgctgcagga
     2701 agatggctga cgggttggcg a
//
LOCUS       DBNHOX010000187         3251 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1236423, whole genome shotgun sequence.
ACCESSION   DBNHOX010000187 DBNHOX010000000
VERSION     DBNHOX010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1236423"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1007)
                     /locus_tag="AC16UZ_04035"
     CDS             complement(99..1007)
                     /locus_tag="AC16UZ_04035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6245885.1"
                     /translation="MKIILLRSSGGAHALRVPRWMGALALAIGLVATAAGAVLGMNLL
                     GSHQMAPETVVHWRQEVEAQRLELQALLDRSTAETQAVGRQLAQMQARLLRMEALGAR
                     VAEVADLDDGEFSFDLPVPVGGPDTTTSEPMPMGDLRADVDALARNLKIRETELEILE
                     SMLRDRDYRAEVAVAGRPVTWGWISSSYGERVDPFSGKMAWHAGLDFAGKQGSDVIAV
                     ASGVVTHAGNRFGYGEMVEVTHGDGSVTRYGHHAEIVVAVGEIVKKGQVVGKMGSSGR
                     STGPHVHFEVLKNGRRVDPARYVVRR"
     gene            1121..1429
                     /locus_tag="AC16UZ_04040"
     CDS             1121..1429
                     /locus_tag="AC16UZ_04040"
                     /inference="COORDINATES: protein motif:HMM:NF017103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HIR6245886.1"
                     /translation="MANLKVDLLLSESQRRFTPLQRLLRQSANQESWTAGLLALLPAS
                     LQNDCRVSDIRNGSIIVVCRNAATATRLRFLAPELLSKLHDLADFRVAQRIEVRVSEF
                     "
     gene            complement(1530..2444)
                     /gene="lpxC"
                     /locus_tag="AC16UZ_04045"
     CDS             complement(1530..2444)
                     /gene="lpxC"
                     /locus_tag="AC16UZ_04045"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HIR6245887.1"
                     /translation="MALNQKTLKNSIRATGVGLHTGDKVFLTLRPAPVDTGIVFVRTD
                     LDGEPRLPARVENVGDTTMATSLTNGTVSVSTVEHLMSAFAGLGIDNAFVDVSAPELP
                     IMDGSAAPFVFLIQSAGVEEQTAPRRFVRIKKAVAFRQGDVVAELKPFDGFRVSYTLV
                     YDHPVFRKHERSATVDLSNTAYVKEVSRARTFGFLADYEQLRSMNLARGGSLDNAVVV
                     DDYRILNGEGLRQADEFVKHKILDAVGDLYLLGCGLIGEFCGYKSGHGSNNALLRRLL
                     ADPEAFEIVTFANEADAPTGFAAPVVAN"
     gene            complement(2647..>3251)
                     /locus_tag="AC16UZ_04050"
     CDS             complement(2647..>3251)
                     /locus_tag="AC16UZ_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018404872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051258 - protein polymerization [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR6245888.1"
                     /translation="AANDVLLGAVQGIADLIIRPGIINVDFADVRTVMSEMGMAMMGT
                     GRGTGDNRARDAAEAAIRSPLLEDVDLHGARGILVNVSAGPDLTIGEYSDVGDIINEF
                     ASEQATVVVGTVIDQDLGDELKVTVVATGLGEPARPNVVIDNTVIEEVIQGQIDYRDL
                     DQPTYKRHNQALHKDNLAEDLDTQDLDYLDIPAFLRRQAD"
BASE COUNT          597 a         1097 c          968 g          589 t
ORIGIN      
        1 cgatgaagga tggagcctgc ccgacaaggc agcgaaggtc ggctgggttc cgggcctgcc
       61 ggcgcgtgtc gagaccagat cggggctgcc ggatcgcgtc agcgacgtac gacgtaacgg
      121 gctggatcga ctcgacggcc gttcttcaaa acctcgaaat gcacgtgcgg gccagtggag
      181 cggccgctgg agcccatctt tccaacgacc tggcctttct tgacgatctc gccgacggca
      241 acaacgattt ccgcgtggtg gccgtatcgg gtgacggacc cgtcgccgtg ggtgacctcg
      301 accatctctc cgtacccgaa gcgattgccc gcatgggtaa ccacgccgct ggccaccgcg
      361 atgacgtcag aaccctgttt gccggcgaag tccaatcccg cgtgccaggc catcttgccg
      421 ctgaacgggt ccacccgctc gccgtacgag gaggagatcc agccccacgt caccgggcgg
      481 ccggccacgg ccacctcagc ccggtagtcg cggtcgcgca gcatggattc aagaatctcc
      541 agctccgttt cgcgaatctt cagattcctt gccagagcgt ccacgtcggc gcgcagatcc
      601 cccatcggca tcggctccga agtggtcgtg tccgggccac caacgggaac gggcagatcg
      661 aacgagaact cgccgtcgtc caggtcggcc acttccgcca cgcgggctcc gagcgcctcc
      721 atgcgcagaa gccgcgcctg catctgggca agctgtcggc cgacggcctg ggtttccgcg
      781 gtggatcgat cgagcagggc ctgcagctcg aggcgctgag cctcgacctc ctggcgccaa
      841 tggacgacgg tttccggggc catctgatgg ctcccgagga ggttcatccc aagcacggcg
      901 cccgcagcgg tcgctacgag gccgatggca agcgccagcg cccccatcca gcggggcacc
      961 cgcagcgcat gagcaccacc cgagcttctc agcaatatga tcttcaccgt cgcgataccc
     1021 ccctgaccgc aacccgaaac ggtggcgaaa cgaaccgccc cgggccaaaa tccatagccc
     1081 gagggcggcc gacacggtcg ttcatgatcg agacccggcc atggcgaatc tcaaagtcga
     1141 tcttctgttg agcgaaagcc aacgccgatt tactcctctc caacgtctcc tacgtcagtc
     1201 ggcgaatcaa gagtcctgga cggccgggct gttagccctg ctgcccgctt cgctgcagaa
     1261 cgactgtcgc gtcagcgaca tccgcaacgg ctcgatcatc gtggtctgcc gaaacgctgc
     1321 aaccgctacg cggttgcgat tccttgcgcc tgaactgctg agcaaacttc acgatctagc
     1381 cgatttccga gtagctcagc gaatcgaggt gcgcgtcagc gaattctgac aggcgcccgg
     1441 ttccttgtgc cctcggctgt gacgattatc ccctcgaccc cagcctcatt ttgagacatg
     1501 tcggtcaaat tggccaatga gtctgcgaat caattagcaa ccacgggagc ggcgaagccc
     1561 gtcggcgcgt ccgcttcgtt tgcgaaggta acgatctcga aagcctcggg gtcagctagt
     1621 aacctgcgca aaagcgcatt gttggatcca tggcctgatt tgtagccgca gaactcgccg
     1681 atcaggccac agccgagcag gtagaggtcg cccaccgcgt cgagaatctt gtgcttgacg
     1741 aactcgtcgg cctgccggag cccctccccg ttgaggatgc ggtagtcgtc caccaccacc
     1801 gcattgtcga ggctccctcc ccgcgcgagg ttcatggatc gcagctgttc gtaatcggcc
     1861 agaaaaccga acgtacgcgc ccgcgacacc tccttgacgt aggcagtgtt ggaaagatcc
     1921 accgtggctg agcgttcgtg cttgcggaat accgggtggt cgtagaccag ggtgtaggaa
     1981 acgcggaacc cgtcgaacgg cttgagctcc gcgacaacgt ctccctgccg gaacgccacc
     2041 gccttcttta tgcgaacgaa tcgtctgggc gcggtctgtt cctctacccc ggcggactgg
     2101 atgagaaaca cgaacggcgc ggcgctgccg tccatgatcg gcaactccgg ggcgctgacg
     2161 tccacgaagg cattgtcgat cccgagcccg gcaaacgccg acatgagatg ctcgaccgtg
     2221 gaaacgctga cggtgccgtt ggtcagcgac gtcgccatgg tggtatcgcc cacgttctcc
     2281 acccgcgccg ggagcctcgg ctcgccgtcg agatcggtgc gcacaaagac gattcccgta
     2341 tccacggggg ccgggcgcag ggtcagaaag accttgtctc ccgtatgcag accaacgccg
     2401 gtggcgcgga tcgagttctt gagcgttttc tgattcagcg ccatgttgcc cgtccttcag
     2461 gccagggccc ctgcaagaag ccgcgaatcg cgctccttct tcctctttta cgcaaagcgc
     2521 tccccgcgca cacgcccttt cgagcctgtg caccgaccgg cacccggcag aaggggatca
     2581 tctgccggcc ggcgctaaat tatggcagcc ggcagccggt aagtcacacg tttcacccgg
     2641 gcacgttcag tcggcctggc gacgcaggaa agccggaatg tccagatagt ccagatcctg
     2701 agtgtccaga tcctctgcaa ggttgtcctt atgcagagcc tggttgtgac gcttgtaagt
     2761 cggctggtcc agatcccggt agtcgatctg cccctggatg acctcctcga tcaccgtgtt
     2821 gtcgatgacc acattgggtc ttgccggctc gccgagcccc gtggccacca cggtgacctt
     2881 cagctcgtcg cccagatcct gatcaatgac cgtccccacc acgacggtgg cctgctcgga
     2941 ggcaaactcg ttgatgatgt cgcccacgtc ggagtactcg ccgatggtga gatccggccc
     3001 ggcagagacg ttgaccagaa tgcctctcgc gccgtgcaga tccacgtcct cgagcagagg
     3061 cgagcggatt gccgcttccg ccgcgtcgcg cgcgcgattg tctcccgttc cccggcccgt
     3121 gcccatcatg gccatgccca tctccgacat gaccgtccgc acgtcggcaa aatccacgtt
     3181 gatgattccc ggccggatga tgaggtcggc aatcccctgc acggccccga gcagcacgtc
     3241 gttggccgca c
//
LOCUS       DBNHOX010000188        12575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1429438, whole genome shotgun sequence.
ACCESSION   DBNHOX010000188 DBNHOX010000000
VERSION     DBNHOX010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12575
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1429438"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="AC16UZ_04055"
     CDS             <1..192
                     /locus_tag="AC16UZ_04055"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family acyltransferase"
                     /protein_id="HIR6245889.1"
                     /translation="SGGFRIEFHAAPPDILAPDPETSAAAMNSAIESLVRTDPSQYQW
                     EYKRFRRQPAGVADPYRVG"
     gene            complement(202..1647)
                     /locus_tag="AC16UZ_04060"
     CDS             complement(202..1647)
                     /locus_tag="AC16UZ_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR6245890.1"
                     /translation="MHFSVILRVTGTLLMLFSIALLLPVVVAVYYGESTTRTFALAFA
                     ITLISGLALTLVARSRRELRSGDGFLITVLFYLGLGLFGAIPIYLAESVDASFTDAAF
                     ESLSGLTTTGATVLTGLDELPRSILFYRQLLQWLGGMGIIVLAVAILPMLGIGGMQLY
                     RAESPGPVKDAKLTPRITETAKALWYLYVGITASCALAYYAAGMDAFDALCHSFSTVA
                     IGGFSTHDASIGHFDSAAVEGVAIFFMILAGINFGLHFMAWNRRNPFLYTRDAEVRLY
                     LIVLVGVAALVCAILASYPDATESPWRDGLFQAVSIATTTGFTTQDFSVWPSAAPVLL
                     MFAAFAGGCAGSTAGGVKMIRVLLIYRQGIREIRRLIHPQGVFPIKLGRHRVPDRVVD
                     AVWGFFSFYVIIFLAMVACLMLISDLDFLTAFSAVGACLNNLGPGLGEVALNYAALPV
                     SVKWLLIFAMLLGRLEIFTLLVLMTPAFWRR"
     gene            complement(1647..3020)
                     /gene="trkA"
                     /locus_tag="AC16UZ_04065"
     CDS             complement(1647..3020)
                     /gene="trkA"
                     /locus_tag="AC16UZ_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016489939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HIR6245891.1"
                     /translation="MKILILGAGQVGGTLAENLAKESIDITLVDNNIEALEELQTKLD
                     IKTVFGSASHPDVLRKAGADDADMLIAVTSSDEVNMVACQVCYSLFRTPQKIARVRSS
                     AYLKGEGFFSGEHMPIDVLINPEEVVTENIRQLLEHPGALQVLGFADDRVQLVAVKAY
                     YGGPLVGQELRFLRQHMPNIDTRVAAIFRRGRAITPEGNTVIEADDEVFFIAAAEHIN
                     AVMSELRRVERPFKRVMIAGGGNIGVKLAQSIEPYFSVKVLEQNARRCEELAEVLDRS
                     VVLNIDATDRELLLEENIEQCDAFCAVTNDDEVNIMSSLLAKRLGVRQVITLIGKPAY
                     VDLMQGGDIDIAISPQQATTSELLTHVRRADVVKVHSLRRGAAEAIEAIAHGDAKNSK
                     VVGRAIGDVDLPTGASIGAVVRGKEVFVGRDDVVVEPDDHVILFLADKRQVAAVERLF
                     QVGLTFF"
     gene            complement(3034..4302)
                     /gene="rsmB"
                     /locus_tag="AC16UZ_04070"
     CDS             complement(3034..4302)
                     /gene="rsmB"
                     /locus_tag="AC16UZ_04070"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR6245892.1"
                     /translation="MPSPAEGAPATASRLLLAVLRDKQTSDQALERIPSTPQVRDTFY
                     GTLRHVFCLQRGVDKALRKPLANKHRDVYCLMLVGAYQLAYARVPDHAAINETVAGCR
                     ALRKPWATGLVNAVLRHIQRKGLPNDCSDASEHPAWMAERIRRDFPDRAGAIFCANMG
                     RAPMCVRINRRLVATDGYRQRLADSGIAFSEGFQDEFIILTAAMPTARLPGFDDGLAA
                     IQDAGAGFAADILAPEPGSRVLDACAAPGGKLFHLVERFPDAATLAIDTSTARLERLE
                     AEAARLHHNVNAQQGDATDLRWWDGQPFDRVLLDAPCTGSGTLRRHPDIRLLRAASDL
                     DDYARLQGRLLTNLWQTLRPGGNLLYCTCSLFAEENDEVIGAFLKAADDAEARPLSLA
                     TGVVRAHGWQLLPTDSRTDGFYYALLTKRV"
     gene            complement(4286..5251)
                     /gene="fmt"
                     /locus_tag="AC16UZ_04075"
     CDS             complement(4286..5251)
                     /gene="fmt"
                     /locus_tag="AC16UZ_04075"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015474830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR6245893.1"
                     /translation="MTRAGGSAPRLGFAGTPEFAAAILQALVTAGHSPVVVYTQPDRR
                     SGRGRKALPSPVKALAAKQGIETRQPATLRETGAAADLAGLELDTLVVAAYGLILPQA
                     ILSAPRYGCMNVHASLLPRWRGAAPVERAIMAGDEETGVTIMQMDAGLDTGPIIEQER
                     CPIESATTGGELEATLARLGARCLLDCLGNLGSLDPTPQDEAGATYAHKLTPADASID
                     WNRPAEAIERQIRALNPRLPATCASGDLRIRILQARAIRDPGGEPGRIVSADRNGIRV
                     SCRESTLVIQSVQLDRGRGRPLSAADAVNGYAAYFEEGTKLAQPS"
     gene            complement(5248..5751)
                     /gene="def"
                     /locus_tag="AC16UZ_04080"
     CDS             complement(5248..5751)
                     /gene="def"
                     /locus_tag="AC16UZ_04080"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010430165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR6245894.1"
                     /translation="MATLDILEYPDPRLRTQAKPVTEVDAQVQQLVEDMFETMYAAPG
                     IGLAASQVNVHQRIIVIDVSENRDEPHVFINPELSLQGAAIESEEGCLSVPGFYEPVI
                     RSEQVIVSALDRDGQSFTLEAQGMLAVCIQHECDHLEGKLFVDYLSNLKRTRIRKKLD
                     KQRRVRA"
     gene            6048..7121
                     /locus_tag="AC16UZ_04085"
     CDS             6048..7121
                     /locus_tag="AC16UZ_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010796361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6245895.1"
                     /translation="MRKLRIIFQVLIGIGLGISASAWSATDGLAQYLKPGHPEVYTVV
                     KGDTLWDISGKFLDRPWLWPEIWRINPQIDNPHLIYPGDQIALEWVEGQPRLSLRRGD
                     AARTYKMTPADSVSLQPQIRSTPLESAIPTIRLEAIQGFLVQNRVVDPKVLSSAPYVV
                     QGENERLVLGAGDRLYVRGVLPDSESFNVVRKGPLYVDPETKEVLGQEATYIGLGRAV
                     AQEDDIATMSLQSTREEAQIGDRVLPTEERRVDSTFFPSAPDGDVNGQIISVFGGVTQ
                     VGQYDVVVLNRGEREGIEVGNVMAIYKRGALVKDRIANQTIRLPSERAGLLMVFRAFE
                     KLSYGLVLETERPLAVNDEVRNP"
     gene            7230..8318
                     /gene="dprA"
                     /locus_tag="AC16UZ_04090"
     CDS             7230..8318
                     /gene="dprA"
                     /locus_tag="AC16UZ_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR6245896.1"
                     /translation="MGDLAGDVESENAAAACLAMELGRPRARAAIAAHGNATSAVEAR
                     AASRDWNPRLVPQRLLADCARHGISLLGGEDACFPELLRHIPDPPLALFSLGALPGRD
                     GTAVAVVGARRCSRLGREVAYQLARQIATAGVRVVSGLALGIDKAAHEGALDAPGAGR
                     TVAVLGCGLARIYPRRNQALAERVVGEGGTLISEYPPEVPPAKHRFPERNRLISGLSH
                     GVVVVEAGDRSGSLITARLALEQGREVMAVPGPVSGGTSRGCHRLLREGAALVECAEH
                     VFEALNLTRPTPGSAETEQRAGLDLGEPLNSVLSAVEPVTTTMDQLVAVTGMAPEALS
                     VCLVDLELAGFVERVHEGYIRRPFSPGI"
     gene            8343..8843
                     /gene="purE"
                     /locus_tag="AC16UZ_04095"
     CDS             8343..8843
                     /gene="purE"
                     /locus_tag="AC16UZ_04095"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR6245897.1"
                     /translation="MSFVAILMGSNSDWPVMQSTTEVLGALDVEFEVRVTSAHRTPAA
                     TMEYVQDATQRGCAVFICAAGMAAHLAGAVAAHTTRPVIGVPIDSGPLSGFDALLSTV
                     QMPGGIPVATVAVGKAGAKNAGYLAAQILGLGDAALADRVASERAENARKVQSQNEQL
                     QEELGR"
     gene            8843..9400
                     /locus_tag="AC16UZ_04100"
     CDS             8843..9400
                     /locus_tag="AC16UZ_04100"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR6245898.1"
                     /translation="MTSVHVARAVRGLKAGGVVLHATEGVWGLACDPFDVEAVARVLV
                     LKGRPVAKGLILVGGNRDHFAEELDLLEPAVRSQVESTWPGAVTWVLPSTRFPEWISG
                     GRETVAVRVSGHPQVRALCERFGGALVSTSANPTGRLPATSQIRARAYFQGRVDYVLP
                     GEVLNAGAPSRIQTLGGEVLRGGGP"
     gene            9397..10260
                     /gene="aroE"
                     /locus_tag="AC16UZ_04105"
     CDS             9397..10260
                     /gene="aroE"
                     /locus_tag="AC16UZ_04105"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014869363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR6245899.1"
                     /translation="MSAGKPGEPDRYAVIGHPVSHSLSPAIHAMFAEQTGQHLAYAKL
                     PAPRDGFARTASDFFAGGGSGLNVTVPFKQEAAGWVDLLEGAAREAGAVNTISLESGQ
                     TKGFNTDGLGLVRDLTVNHGLSISGARVLVVGAGGATQGVVRPLIALGPQALTVANRT
                     VSKAAELVGSLGSAAGRARVAACAPEAATGPFDLVINATSAGLEGHGEVVPADAAAGA
                     VCYDMVYSTGAGAATAFCEWALGVGAVRAIDGLGMLVEQAAEAFLIWRGVRPDTAPVI
                     DALRRVAPPAY"
     gene            10281..10520
                     /locus_tag="AC16UZ_04110"
     CDS             10281..10520
                     /locus_tag="AC16UZ_04110"
                     /inference="COORDINATES: protein motif:HMM:NF021072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HIR6245900.1"
                     /translation="MTIKKRFIAGAVCPDCGAQDRLVVEEMPDGKRRRCVSCGFTDVS
                     ASEPEVALPTRFARRGVDSEQARPVRLLGEGDSDS"
     gene            10898..12013
                     /gene="coxB"
                     /locus_tag="AC16UZ_04115"
     CDS             10898..12013
                     /gene="coxB"
                     /locus_tag="AC16UZ_04115"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR6245901.1"
                     /translation="MGLGLAAAARADELNMTPGVTQISRSIYDLHMVIFWICVAIGVV
                     VFGVMFWSIFQHRKSRGAQAASFHESTRLEIAWTLIPILILIGMAFPATQTLVAMYDT
                     GGEDMAVEVRGYQWKWQYKYLDEDYNSTFSFFSNLATSRDEIENRVVKNDNYLLEVDE
                     PLRIPANRKVRFLITSEDVIHAWWVPDFGIKRDAIPGMLNELWTVVETPGVYRGQCTE
                     LCGMDHGFMPIVVEVMAEEDYDAWYADRVRMEEARHEALSKTFTPAELMEEGERVYAT
                     FCASCHQANGKGLPPVFPALAGSPIATGPLSDHLDIIVNGRAGTSMNAFGNQLDAAEI
                     AAVTHYERNAWGNDVGDVTQPIDVIEFMSGKEAEETP"
     gene            12010..>12575
                     /locus_tag="AC16UZ_04120"
     CDS             12010..>12575
                     /locus_tag="AC16UZ_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR6245902.1"
                     /translation="MSEAVLTHHDDHHHGPASGITRWLFTTNHKDIGTLYLWFSFLML
                     LIGGAMALVIRTELFQPGLQFVEPAFFNQMTTNHGLIMVFGAIMPAFVGLANWLLPMM
                     VGAPDMALPRMNNLSFWILPFAFGMLISTLFMEGGGPNFGWTFYAPLSTTYAPDTVTY
                     FIFGVHLMGASSIMGSINIIATILNMRAP"
BASE COUNT         2160 a         3921 c         4268 g         2226 t
ORIGIN      
        1 agcggcggct tccggatcga atttcacgcc gcgcccccgg acatcctcgc gccggatccc
       61 gagacttcag cggctgccat gaactcggcc atcgagtcgc tcgtccgcac cgacccgtcc
      121 cagtaccagt gggagtacaa gcgattcagg cggcaacccg caggagttgc ggatccctat
      181 cgcgtcgggt aaccgggcgc ttcagcggcg ccagaaggct ggggtcatca gaacgagcag
      241 cgtgaagatc tccagtcggc ccagcagcat cgcgaagatc agcaaccact tcaccgatac
      301 ggggagcgcg gcgtagttga gcgccacctc gccgaggccg ggccccaggt tattcaggca
      361 ggcgcccacc gccgagaagg cggtgaggaa atccagatcg gagatcagca tcaggcaggc
      421 gaccatggcc agaaagatga tcacgtaaaa cgaaaagaac ccccagacgg cgtcgacgac
      481 acggtcgggg acgcgatgtc ggcccagctt gatggggaat acgccctggg ggtgtatgag
      541 ccggcggatc tcgcgaatgc cctggcggta gatgagcaac acgcggatca ttttgacccc
      601 gcccgccgtc gagccggcgc accctccggc gaacgcggcg aacatcagca ggacgggcgc
      661 ggccgagggc cagacgctga agtcctgggt ggtaaaaccg gtggtcgtcg cgatggatac
      721 ggcctgaaag aggccgtccc gccacgggga ctccgtggcg tccggatagc ttgcgagaat
      781 ggcgcagacc agcgccgcga ctcctaccag aacgatcagg tagagacgaa cctccgcgtc
      841 acgcgtgtac agaaacgggt tgcgtcggtt ccatgccatg aagtgcaggc cgaaattgat
      901 cccggcgaga atcatgaaga agatggccac tccttccacg gccgcgctgt cgaagtggcc
      961 gatgctggcg tcgtgggtcg agaagccgcc gatggcgacg gtggaaaagc tgtggcaaag
     1021 ggcgtcgaag gcgtccatcc cggcggcgta gtaagcgagc gcgcaggatg ccgtgatgcc
     1081 gacgtagaga taccacaggg ccttcgccgt ctcggtgatg cgcggcgtta gcttggcatc
     1141 cttgaccggc cccggggact cggcgcgata cagctgcatg ccgccgatgc ccagcatggg
     1201 aaggatcgcc accgcgagaa cgatgatccc cataccgccg agccactgaa gcagctgccg
     1261 gtagaaaagg atggaacggg ggagctcgtc gagcccggtc aggaccgtcg ccccggtggt
     1321 cgtcagcccc gacagcgact cgaaagcggc gtcggtgaaa gacgcatcga cgctctcggc
     1381 caggtagatg gggattgccc cgaacaggcc gagacccagg tagaagagca cggtgatgag
     1441 aaacccgtcg ccgcttcgca actcgcgccg ggaccgtgcg accagggtca gcgccaggcc
     1501 cgaaatcagg gtgatggcga aagccagggc aaacgtgcgg gtggtgctct ccccgtagta
     1561 gacggccacc accacgggca gcaggagcgc aatgctgaac agcatcagca gcgttccggt
     1621 cactcggaga atgacggaga aatgcatcag aaaaaggtca gcccgacctg gaacagccgt
     1681 tccaccgccg ccacctgacg cttgtccgcc agaaacagga tcacgtggtc gtcgggctcc
     1741 accaccacgt cgtcacggcc gacgaaaact tccttgcccc ggaccacggc gccgatggac
     1801 gcgccggtcg gcaggtcgac atccccgatc gcccggccaa ccaccttgga gtttttcgcg
     1861 tcgccgtggg cgatggcctc gatggcttct gcagcaccgc ggcgcaggct gtgcaccttg
     1921 accacgtcgg cccgtcgcac gtgggtcagg agctcgctgg tggtggcctg ctgaggcgag
     1981 atggcgatgt cgatgtcgcc gccctgcatc aggtcgacgt aggcgggctt gccgatgagc
     2041 gtgatcacct gtcggacccc gagccgtttg gccagcaacg aggacatgat gttgacctcg
     2101 tcgtcgttgg tgacggcgca gaaggcgtcg cactgctcga tgttctcctc cagcagaagc
     2161 tcccggtcgg tggcgtcgat gttgaggacc acggaccggt cgagaacctc ggccagctcc
     2221 tcgcagcggc gggcgttctg ctccaacacc ttcaccgaga agtaaggctc gatgctctgg
     2281 gcaagcttga cgccgatgtt gcccccgccg gcgatcatca cgcgcttgaa gggccgctcc
     2341 acccgcctga gctcgctcat cacggcgttg atgtgctcgg cggcagcgat gaagaagacc
     2401 tcgtcgtctg cctcgatcac cgtgttgccc tcgggcgtga tggcgcgacc gcgacggaag
     2461 atggccgcca cgcgggtgtc tatgttcggc atgtgctggc ggagaaagcg aagctcctga
     2521 ccgaccaggg ggccgccgta gtacgccttc acggccacca gctggactct gtcgtcggcg
     2581 aacccgagaa cctgaagcgc tccggggtgc tcgagcagct gtcggatgtt ttctgtgacg
     2641 acctcctctg ggttgatcag cacgtcgatg ggcatgtgct ccccgctgaa gaacccttcc
     2701 cccttcagat atgctgacga gcgaacccgc gcgattttct gcggcgttcg aaacagggag
     2761 tagcagacct ggcaggcgac catgttgacc tcgtcgctgg aggtcaccgc aatcagcatg
     2821 tcggcgtcgt cggcgccggc cttgcgcagc acgtccgggt ggctcgcgga cccgaagacg
     2881 gtcttgatgt ccagcttggt ctgcagctcc tcgagggctt cgatgttgtt gtcgaccagc
     2941 gtgatgtcga tggattcctt ggcgaggttc tcggccagcg ttccgcccac ctgccccgct
     3001 ccgagaatga gaatcttcat gttggctgcc ttgttacacc cgcttggtca gaagagcgta
     3061 gtagaacccg tcggtgcggc tgtcggtggg cagaagctgc cacccgtgag ccctgaccac
     3121 gccggtagcc agcgacagag ggcgggcctc ggcatcgtca gccgctttga ggaacgcccc
     3181 gatcacctca tcgttctctt ccgcaaacag cgagcacgtg cagtaaagaa ggttaccgcc
     3241 ggggcgcagg gtctgccaca ggttagtcag cagccgtccc tgcagtcggg cgtagtcgtc
     3301 gaggtccgag gctgcccgca ggagccttat gtcggggtgc cggcgcaggg tcccgctgcc
     3361 cgtgcagggg gcgtccagca ggacccgatc gaagggctgg ccatcccacc acctcaggtc
     3421 cgtggcatcc ccctgctggg cgttcacgtt gtgatggagg cgagcggcct cggcttcgag
     3481 ccgttccagg cgtgccgtgg aggtgtcgat ggccagggtg gccgcgtccg ggaaccgctc
     3541 gaccaggtgg aagagctttc cgcccggggc cgcgcaggcg tcgagcaccc tggaccccgg
     3601 ctccggagcc aggatgtctg cggcgaatcc ggcgcccgcg tcctggatgg ccgccagccc
     3661 atcgtcgaag cctggcagcc gggccgtcgg catggccgcc gtcagaatga tgaactcgtc
     3721 ctgaaagccc tccgagaagg cgatgccgga atccgcgagc cgctgtcggt agccgtccgt
     3781 cgcgacgagt ctcctgttga tgcgaacgca catgggcgca cggcccatgt tggcgcagaa
     3841 gatggcgccc gcccggtccg gaaagtctcg acgaattcgt tcagccatcc aggccggatg
     3901 ctccgaagca tccgaacaat cgttcggcag accctttcgc tggatgtggc ggagaacggc
     3961 gttgaccaga cccgtagccc agggcttgcg aagcgcccgg cacccggcga ccgtctcgtt
     4021 gatggcggcg tggtccggta cgcgggcgta tgccagttgg taagcgccca ctaacattaa
     4081 acagtagacg tcccgatgct tgttcgccag aggcttgcgc agcgccttgt ccacaccgcg
     4141 ttgcaggcag aaaacgtgtc gcagggtgcc gtagaacgtg tcgcggacct gcggtgtgga
     4201 cgggatgcgc tccagagcct gatcggacgt ctgcttgtcc ctgagcacgg cgagcagcag
     4261 gcgcgacgcc gtggccggag cgccttcagc tgggctgggc aagctttgtg ccttcttcaa
     4321 aataggcggc gtagccgttg acagcgtcgg ctgcggacag gggacgcccg cgcccgcgat
     4381 cgagttgcac ggactggatg accagggtgc tctccctgca ggagacgcgg ataccgttcc
     4441 tgtcagcgga gacgatcctt ccgggttccc cgccagggtc ccggatcgcc cgcgcctgga
     4501 gaatgcggat gcgcaggtcg ccggaggcgc aggtggcggg caatcttgga ttcagagctc
     4561 ggatctggcg ctcgatcgct tcggcggggc ggttccagtc gatgctggca tcggccggcg
     4621 tcagcttgtg ggcgtacgtc gccccggcct catcctgggg cgtggggtcg aggctgccca
     4681 gattccccag gcagtccagc aggcagcggg cgccaagccg cgccagcgtt gcttcgagct
     4741 cgccgccggt ggttgcgctt tcgatggggc acctctcctg ctcgatgatc gggccggtgt
     4801 ccagaccggc gtccatctgc atgatggtaa cccccgtctc ctcgtccccc gccatgatgg
     4861 ctcgctcgac cggcgcggcc ccgcgccagc gcggcagcag cgaggcgtga acgttcatgc
     4921 acccgtacct gggcgcgctc aggatggcct gggggaggat cagtccatat gcagctacca
     4981 ccagcgtgtc gagctcaagc cccgccagat ccgccgcggc cccggtctcc ctcagcgtgg
     5041 ccggctggcg ggtctcgatg ccctgcttcg cggccagggc cttgacgggg ctcgggagcg
     5101 ccttgcggcc acgaccggag cgccgatcgg gctgggtgta cacgaccacc ggcgagtggc
     5161 cggccgtgac gagcgcttgg aggatggcgg ccgcgaactc gggtgtgcct gcaaacccaa
     5221 ggcgcggcgc gctgccgccc gccctggtca tgcgcggacg cggcgttgtt tgtccagctt
     5281 tttgcgaatc cgcgtgcgct tcaggttgga caggtaatcc acgaagagct tgccctccag
     5341 gtggtcgcat tcatgctgga tgcagacggc cagcatgccc tgggcctcga gcgtaaagga
     5401 ctgtccatcc ctgtcgagcg cgctcactat tacctgctcg gagcggatga ccggctcgta
     5461 gaaccccgga accgacaggc aaccctcctc cgactcgatg gcggccccct gcagcgaaag
     5521 ctcgggattg atgaaaacgt gcggctcgtc tcggttctcg gaaacgtcga tcacgatgat
     5581 ccgttggtgg acgttgacct ggctggccgc caggccaatt ccgggagcgg cgtacatggt
     5641 ctcgaacatg tcttcgacca gttgctgtac ttgcgcgtcc acctcagtca cgggtttggc
     5701 ttgagtgcgc agcctggggt cgggatattc gagtatgtcg agggttgcca tggattgttg
     5761 tagcgccttt gcggcctatg tgatgtgtct ctacttctgc tgcaattcag gctgataaca
     5821 ttgtgacgag tatatgtttt tcgctcggaa acccggctcg gtcgcgggct caagccttac
     5881 ccggcgacgg ttgagcggat gttaatgagc cttcatttgg acttaaaggc ctacaactcg
     5941 aaatggacag catccggcgg caagagttcg aagcgagcca gcctcggcga tcctcttcag
     6001 ggatgcttcc ggcacgacta gcaggtacag ctaaaaggaa aggaatcatg aggaaactgc
     6061 gcatcatttt ccaggttctg atcggaatcg gtcttgggat ctcggcaagc gcatggagtg
     6121 ccacggacgg cctggctcag tacctcaagc cggggcatcc ggaggtttac accgtggtca
     6181 agggcgacac gctgtgggat atcagcggca agttcctgga tcggccctgg ctttggccgg
     6241 agatctggcg gataaacccc cagatcgata atccccatct catctacccg ggcgatcaga
     6301 tcgcgctcga gtgggtggag ggacagcccc ggttgtctct gagaaggggt gacgcggcgc
     6361 gaacctacaa gatgacgccg gcggattcgg tgagcctgca accgcagatt cgctccaccc
     6421 cgctggagtc ggccatcccc accattcggc tggaggccat tcaggggttc ctggtgcaga
     6481 atcgggtggt ggatcccaag gttctgtcca gcgcgccata cgtcgtgcag ggcgaaaacg
     6541 agcgcctggt tctgggcgcc ggcgaccggc tctacgtgcg gggcgtgctg ccggacagcg
     6601 aaagcttcaa cgtcgtccgc aaagggcccc tttatgtgga cccggagacg aaagaggtgc
     6661 ttggccagga ggctacctac atcgggctgg gcagggccgt ggcgcaggaa gacgatattg
     6721 ccaccatgtc gctgcagagc acccgcgagg aagcccagat cggtgaccga gtgctgccaa
     6781 ccgaagagcg gcgggttgac tccacgttct tcccgagcgc gccggacggg gacgtcaacg
     6841 gccagatcat ttccgtgttc ggtggcgtga cccaggtcgg ccagtacgac gtggtggtgc
     6901 tcaatcgcgg cgagcgggag ggcatcgagg tcggcaacgt catggcgatc tacaagcgcg
     6961 gcgctcttgt caaggaccgt atcgccaatc agaccatccg gctccccagc gagcgggccg
     7021 gtctgctgat ggtcttccgg gcgttcgaga agctctctta cggcctggtg ctcgagaccg
     7081 agcgtccgct cgccgtcaac gacgaggtac gtaatccctg atttgcgcta cggtgcggcg
     7141 aaatcgcacc gctgaccggc cgccgggctg accggcggca atcagggtct tcaatagaat
     7201 cgcccctcaa gtcgaggggc gattttttta tgggggacct ggctggggac gtggagtcgg
     7261 agaacgcggc ggcggcctgc cttgccatgg agcttggtcg gccgcgggca cgggcggcaa
     7321 tcgccgctca tggcaatgcg acctcggcgg tcgaagctcg ggcagcgtcc cgggattgga
     7381 acccccgcct ggtaccccaa cggctgctcg cggattgtgc ccggcacggg atctcgctgc
     7441 tcggcgggga ggatgcgtgc tttcccgagc tgttgcggca catccccgac ccgccgcttg
     7501 cactgttctc tctcggcgcg ctgccgggac gagatgggac tgccgttgcc gtggtgggcg
     7561 ccaggcgttg cagccggctc ggccgggagg tcgcctacca gctcgcccgg caaatcgcga
     7621 cggcgggcgt cagggtggtc agtgggctgg cgctcggtat cgataaagcc gcccacgaag
     7681 gcgcgctcga tgcccctggg gccggtcgga ccgttgcggt cttgggctgc gggcttgccc
     7741 ggatctatcc gcggcgcaat caggccctcg ccgagcgcgt ggtgggcgag ggcggcacat
     7801 tgatcagcga gtacccgccg gaagtgcccc ccgcgaagca tcggtttccc gagcgcaaca
     7861 ggctgatcag cgggctctcc cacggcgtgg tcgtggtcga ggccggcgat cgcagcggct
     7921 ctctgatcac cgcgcgactg gcactcgagc aggggcggga ggtaatggcc gttcccggac
     7981 cggtgagcgg cgggacgagt cgaggctgcc atcgcctgct gcgggagggg gccgcgctgg
     8041 tggagtgcgc cgagcatgtc ttcgaagcgc tgaacctgac ccgccccacc cccggatccg
     8101 ccgagacgga acaaagggcg ggtttggacc tgggcgagcc tctgaacagc gtgctgtcgg
     8161 cggtggagcc tgtgaccacc accatggacc agctggtcgc cgtcaccggc atggctcccg
     8221 aggccctgtc ggtgtgtctg gtcgatctgg agctcgcggg atttgtcgag cgggtccacg
     8281 agggttatat tcggcgacct ttttcgccgg ggatataaat ttccggctca ggaggaaaaa
     8341 acatgagctt tgtggccatt ttgatgggtt ccaactccga ctggccggtg atgcagagca
     8401 ccaccgaggt gctcggcgcc ctggacgtgg agttcgaggt gcgggtcacc tctgcccacc
     8461 ggaccccggc ggcgaccatg gagtacgtgc aggacgcgac ccagcgagga tgcgcggtct
     8521 tcatctgcgc cgccggcatg gcggcccatc ttgcgggtgc cgttgcggcg cacaccaccc
     8581 gaccggtgat cggggtgccc atcgacagcg gcccgctttc cggattcgac gcgttgctct
     8641 ccacggtgca gatgccgggc gggatcccgg tggcgaccgt ggccgtgggc aaagcagggg
     8701 cgaagaacgc cggttacctg gccgcgcaga tcctgggact gggtgatgcg gccctcgccg
     8761 accgggtcgc ctccgagcgg gcggagaacg cacgcaaagt ccagtcccag aacgagcagc
     8821 tgcaggaaga actcggccgc tgatgacttc cgtgcacgtg gcgcgcgccg tccgggggct
     8881 taaggctggc ggcgtcgtgc tgcacgcgac ggagggtgtc tggggccttg cctgcgatcc
     8941 tttcgacgtg gaagcggtgg ctcgcgtact ggtgctcaaa ggtcgcccgg tggccaaggg
     9001 cctgatcctt gttggcggga acagggatca cttcgctgag gagctcgacc tgctcgagcc
     9061 ggctgtccgg tctcaggtcg aaagcacctg gcccggtgcg gtgacctggg tccttcccag
     9121 cacccgcttt ccggagtgga tcagcggcgg tcgggaaacg gtagccgttc gggtctcggg
     9181 tcatccccag gtcagagcgc tgtgcgaacg ctttggcgga gccctggtgt ccacctcggc
     9241 gaaccccacc ggcagattgc cggccaccag ccagatcagg gcacgagcgt acttccaggg
     9301 gcgcgtggat tacgtcctcc ccggcgaggt tctcaacgcg ggcgccccca gccggatcca
     9361 gaccctgggt ggcgaggtgc tgcgcggagg aggcccgtga gcgccggaaa gccgggggaa
     9421 ccggatcgct acgcggtgat cggccatccg gtgtcgcatt cgctctcgcc cgcgatccac
     9481 gccatgttcg cagagcagac cgggcagcat ctggcgtacg caaagctgcc ggccccgaga
     9541 gacggttttg cccggacggc cagcgacttc tttgccggcg gtgggagcgg gctcaacgtg
     9601 accgtgcctt tcaaacagga agccgccggg tgggtcgatc ttctggaagg ggccgcccgg
     9661 gaggcggggg ccgtcaacac catctccctt gaatcggggc agaccaaagg cttcaacacc
     9721 gatggcctgg gtctcgtcag ggatctcacc gtgaatcacg ggctttccat ctccggtgcc
     9781 cgggtgctcg tggtcggtgc cggcggcgcc acccaggggg tggtgcgtcc tctgatcgcc
     9841 ctcggccccc aagcgctgac cgtcgccaac cgcactgtct cgaaagccgc tgaactggtc
     9901 ggttcgctgg gctctgccgc cggccgcgca agggtggcag cctgcgcgcc agaggcggcg
     9961 acgggtccgt tcgatctggt tatcaacgcc acctcggcag gtttggaagg tcacggcgag
    10021 gtcgtgcccg ccgacgcggc tgcgggggca gtgtgttacg acatggttta cagcacgggg
    10081 gcgggcgctg ccacggcctt ctgcgagtgg gcgctcggcg tgggggcggt tcgcgccatc
    10141 gacgggctcg gcatgctggt agagcaggcg gccgaagcct ttctgatctg gcgtggggtc
    10201 aggccggaca ccgcgcccgt catcgacgcg ctccgtcgcg ttgcgccgcc ggcgtattga
    10261 acaggaatgg agctttcggc ttgacgatca agaagcgatt cattgcgggg gccgtttgcc
    10321 ccgattgcgg cgcgcaggat cgcctggtcg tcgaggaaat gcctgacggt aagcgccgcc
    10381 gctgcgtgag ctgcggtttc accgacgtca gcgcgtctga gccggaagtg gctttgccga
    10441 cgcggtttgc gcgaaggggc gtcgattcgg agcaggcgcg ccccgtgcgt ctgctgggcg
    10501 aaggcgattc agactcctga tcgccgggca cttttgttcg actaaggcgt tgaaaacctc
    10561 acaatttcga ctgccttcgt tgtgatctgg cggcgcgctg ctataatgcg cccgctcttc
    10621 ggttgctctg ccgacgaaga gcttccagga agcaccttca agcccgtttt cgggcacgac
    10681 caagaggtca cggtctgacc gccgtccgcc ccccaattgg gaggtggacg ctttgactcg
    10741 accgggtcgc tggtgcgccg aggcgcaact ctttagcggc tcccttatca ggcgaccgac
    10801 ggtcgccggg ggtctttgtt gcttccgaag atgccacaac ctaacgcaag ggtgagcagg
    10861 tttgaaacat tactggcatg ggctgccgac gtgcctgatg ggccttggac tggccgccgc
    10921 tgcgcgagcc gacgagctga acatgacgcc gggagtgacg cagatcagtc gctccatcta
    10981 cgaccttcac atggtgatct tctggatctg cgtcgccatc ggcgtggtgg ttttcggggt
    11041 catgttctgg tcgatttttc agcacaggaa gtcgcggggc gcgcaggcgg ccagcttcca
    11101 tgaaagcact cgcctggaga ttgcctggac gctgattccg atcctcatcc tcatcggcat
    11161 ggcctttccg gccacccaga ccctcgtcgc catgtacgac acgggcggag aggacatggc
    11221 ggtcgaggtg cgtggctatc agtggaagtg gcagtacaag tatctggacg aggactacaa
    11281 cagcacgttc agcttcttca gcaacctggc aacctccagg gacgagatcg aaaaccgtgt
    11341 ggtgaagaac gacaactacc tcctggaggt cgatgagccg ctcaggatcc cggcgaatcg
    11401 gaaggtgcgc tttctcatta cctcggagga cgtgatccac gcgtggtggg ttccggattt
    11461 cggcatcaag cgagacgcga tcccaggcat gctcaacgag ctgtggaccg tcgtggagac
    11521 gccgggcgtt taccgggggc aatgcactga gctttgcggt atggaccacg gcttcatgcc
    11581 catcgtcgtc gaggtcatgg cggaggaaga ctacgacgcc tggtatgcgg accgggtgcg
    11641 catggaggaa gcgcgccacg aagcgctctc caagacgttt acgccggcgg agctcatgga
    11701 ggagggggag cgcgtctacg cgacgttctg cgcaagctgt catcaggcaa acggcaaagg
    11761 gctgccaccg gtgttcccgg cccttgccgg aagccctatc gccacggggc cgctcagcga
    11821 ccatctggac atcatcgtca acggccgtgc cgggacgagc atgaatgcct ttggcaacca
    11881 gctggacgcg gcggagatcg cggccgtgac tcattacgag cggaacgcct ggggtaacga
    11941 cgtgggcgac gttacgcagc ccatcgatgt catcgagttc atgtctggga aggaagccga
    12001 ggaaacgcca tgagtgaagc cgtactgact catcacgacg accatcatca cgggcccgct
    12061 tccggaatca cccgctggct gttcaccacg aaccacaagg acatcggtac cctctacctc
    12121 tggttcagct tcctgatgct gctcatcggc ggcgccatgg cgctggtgat acgtacggag
    12181 ctgttccagc cggggctgca gttcgtggaa cccgccttct tcaaccagat gaccacgaac
    12241 cacggcctga tcatggtctt cggggccatc atgccggcct tcgtcggtct ggcgaactgg
    12301 ctgctgccga tgatggtggg cgcgcccgac atggcgctgc cgaggatgaa caacctgtcg
    12361 ttctggatcc tgccgttcgc cttcggcatg ttgatctcga cgctgttcat ggaggggggc
    12421 gggccgaatt tcggatggac gttctacgcc cccctgtcga cgacctatgc gccggacacg
    12481 gtgacctatt tcattttcgg cgttcacctg atgggtgcgt cctccatcat ggggagcatc
    12541 aacatcatcg ccaccatcct gaacatgcgc gcgcc
//
LOCUS       DBNHOX010000189         1917 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_466189, whole genome shotgun sequence.
ACCESSION   DBNHOX010000189 DBNHOX010000000
VERSION     DBNHOX010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_466189"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..947)
                     /gene="prfB"
                     /locus_tag="AC16UZ_04125"
                     /pseudo
     CDS             complement(1..947)
                     /gene="prfB"
                     /locus_tag="AC16UZ_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153889742.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            complement(1000..>1917)
                     /locus_tag="AC16UZ_04130"
     CDS             complement(1000..>1917)
                     /locus_tag="AC16UZ_04130"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF037628.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR6245903.1"
                     /translation="VARRDAGKLTAADLGFALGPRLNAAGRLDDMSLGIRCLTAADPG
                     SARELTTALDELNRARRQLQEEMVSDAELLVAGHDVAVEDRYGVCVYDATWHQGVVGI
                     VAGRLREKLHRPVIAFAEAGDLAPDELKGSARSLPDLHVRDALDAVATRYPGMLVNFG
                     GHAMAAGLTLKRVHYEAFARAFDAEVRRVLPAESLQAVIVTDGRLEDEELSLDVARLL
                     AAAGPWGQGFPEPCFHDEFTVVSQRVVGESHLKLVLKRGERLFDAIAFNQPPLPDVQR
                     VAAVFRLSENDYGALPTLQLMVEHLVGLA"
BASE COUNT          324 a          664 c          592 g          337 t
ORIGIN      
        1 cggccttcga gtcctcgacg gcctgccgct ccgcgttgcg ttcgagctcg gccagctcgt
       61 agagttttgc gcgcagctgc ttgaatgcct tgtccttgtt ctggtgctgc gaacgctcgc
      121 tctggcactg cacgaccgtg ttcgttggca ggtgggtcag gcgaacggcc gagtccgtcc
      181 ggttgacgtg ctggccgccg gccccgctgg cccgatacgt gtccacccgc acatcggcag
      241 gattgatatc gatctcgatg tcgtcgtcga tctcgggcgc gacgaaaacc gaagcaaacg
      301 aggtgtgacg ccgattaccg gagtcgaaag gcgactttcg aaccagccgg tgcaccccgg
      361 tttccgttcg caaccagccg aacgcgtgat caccctggaa ctgcaccgtg gcccccttga
      421 tgccggcaac ctcgccgggc gatagctcgg tgatctcggt gtcgaagccg tgcttttctc
      481 cccagcgcag atacatgcga aggagcattt ccgcccagtc ctgggcctca gtgccgccgg
      541 atccggactg aatctcgacg tacgcgttcg cctcgtccat ctcgccctgg aacatgcgtc
      601 ggaactccag ggattcgagc tgccgctcga gatcgtcgag ctcctcgtcg acctccgtca
      661 gggcatcgtg gtcttcttcg gtagacgcaa gctcggccag atcccgcagg tcattgaggg
      721 tctcgtgcag atcgcgcagg cctttgacca cctgctccag cgccgagcgc tccttgttga
      781 ggcgctgcgc ttcttcagga tcgttccaga cgtcgggctc ggcgagggtc agctcgacct
      841 cggcgagacg gtgttccttc tcggcaaagt caaaggtacc ccctaagcgc gacgtcgcgc
      901 tcgctgaggt ccttgatacg ctctcgcacc gacgtgatct cggccattat ccccgctctc
      961 gacagacctg cggcgcccgg ccgcaaaaga cgcgcaagtt tatgccaacc ccaccagatg
     1021 ctccaccatg agctgaagcg tgggcagcgc gccgtagtcg ttctcgctga gacggaacac
     1081 cgcggccacg cgctgaacgt cgggcagcgg cggctggttg aaggcgatgg cgtcgaagag
     1141 ccgttccccg cgtttgagca ccagcttgag atgggactcc ccgaccaccc gctggctgac
     1201 cacggtgaac tcgtcgtgaa agcagggctc cggaaacccc tggccccagg gcccggcagc
     1261 ggcgagcagg cgggcgacgt ccaggctcag ctcctcgtcc tccagccgtc cgtccgtcac
     1321 gatcacagcc tgcagggact ccgccggaag cacccgccgg acttcggcgt cgaacgcccg
     1381 ggcaaaagcc tcgtagtgca cgcgtttcag agtcagcccg gccgccatgg cgtgaccacc
     1441 gaaattgacc agcatcccgg ggtaacgagt ggcaacggcg tccagagcgt cccttacgtg
     1501 cagatcgggc agcgagcggg cggaaccctt gagctcgtcc ggcgcgagat cgccggcctc
     1561 tgcgaaggcg atgaccgggc gatgcagctt ctctctcagc cggccggcca cgatgccgac
     1621 aacgccctga tgccaggtcg cgtcgtaaac acagacaccg taacgatcct caacggcgac
     1681 gtcgtggccg gccaccagca attcggcgtc gctgaccatc tcttcctgca gctgccgccg
     1741 ggcgcgattg agctcatcca acgccgtggt gagctcacgc gccgaaccgg gatcagcagc
     1801 tgtcaggcag cgtatgccca ggctcatgtc gtccagccgg ccggccgcat tcagccgagg
     1861 gcccagtgca aagccgagat cggccgccgt cagctttccc gcgtcgcggc gcgccac
//
LOCUS       DBNHOX010000190         2289 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1494291, whole genome shotgun sequence.
ACCESSION   DBNHOX010000190 DBNHOX010000000
VERSION     DBNHOX010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1494291"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="AC16UZ_04135"
     CDS             <1..286
                     /locus_tag="AC16UZ_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245904.1"
                     /translation="KTLIVTATAFAYGLPASTAKTAQHALEVRTLGGTWLQQTDQFID
                     SGQHFSPSGNNGTFIPERSDAKARVTSTVNNLPVSTTVWGFLVDKGYEGG"
     gene            287..>2289
                     /locus_tag="AC16UZ_04140"
     CDS             287..>2289
                     /locus_tag="AC16UZ_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245905.1"
                     /translation="MASPKTLAELVQQFGEHLNPNRVPVSAPLGGGQVKPRVGPLGGA
                     AGATAAGLLGFGASEDAEAAPFGTLARRLGYGTQEAWHGSPYMFEDDRLLGSMKGRGE
                     GNAAYADGRYVSLAQGVGQEYKDSLAGFANELQFPDRVERLPNANDYYAARYGDNSPA
                     WQEFEQKVGTRGAGKAVLDSTIRAAATEETPLEALEYVLQSKKVDPQDAELARRYLSG
                     ATGAREFESGALYGVEIPETQRLLDWDIPLASQPEEIKQPLSQVWSDLYLASATGQDL
                     SAIARMDPVQRRLAKERLQVPPLPEGTTGEQLYLQLAEQLGGKGQASDYLNSKGIPGT
                     TYIGVESQERNAVLFNDDATNVFSKDGQPFGKAELPVPEVEELDMGAFRDMFSGRAPQ
                     VAAGAGAAGLLASQGASAEALQADAQRRAAEAYDPVAALRAQPTYQQPATDGLTQTLQ
                     GSAEQIAGQELAANRKRAQERAGGLVEAGTGLLGDIGQAIPKNIRENPVASLAGEFTG
                     LPGLYRAGEEYLSGVERISPEAAIDVASAAVPFLPVARGLRAADRAVTAADEAGRASV
                     AGTSLRPGTEGYRGATASLDQARRVDEPRVEIPRLRSANKGRPKGTIVGLPKGLEPTE
                     ANVERLADEYAGRVMKGIENGVQPGYFYKEGSQNVRDWTGNDP"
BASE COUNT          411 a          771 c          809 g          298 t
ORIGIN      
        1 caagaccctg atcgtcacgg ccaccgcgtt cgcctacggc ctaccggcgt cgaccgccaa
       61 gaccgcgcag cacgccctgg aggtgaggac gctcggtggc acctggctgc aacagaccga
      121 ccagttcatc gacagcgggc agcacttcag cccgtctggc aataacggca ccttcatccc
      181 cgagcggtca gacgccaagg cgcgcgtcac gagcacggtc aacaacctgc cggtctcgac
      241 gaccgtctgg ggattcttgg tcgacaaggg ttacgaggga ggctaagtgg cgagtccgaa
      301 gaccctggca gagctggtac agcagttcgg agagcacctg aaccccaaca gggtgcctgt
      361 gtccgccccc cttggtggtg ggcaggtgaa gcccagggtc gggccgctgg gcggagctgc
      421 cggcgccacc gcagccggcc tgctcgggtt tggcgcgagc gaggacgccg aggcagcccc
      481 gttcggcacc ctggcacgac gcctggggta cggcacgcag gaagcctggc acggcagccc
      541 ctacatgttc gaggatgacc gcctcctggg ctcgatgaag ggccgagggg agggcaacgc
      601 agcctacgcc gacgggcgct acgtgagcct ggctcaaggg gtgggtcagg agtacaagga
      661 ctccctcgcg ggcttcgcca acgagctgca gttccccgac cgggtggagc ggctgccaaa
      721 cgcgaacgac tactatgcgg cgcgctacgg cgacaacagc cccgcctggc aggagttcga
      781 gcagaaggta gggactcgag gcgctggcaa ggcggtcctg gactctacca tcagggccgc
      841 ggctaccgag gaaacgcccc tggaggccct cgagtacgtc ctgcagtcga agaaggtcga
      901 tccccaggac gcggagctgg cccggcgtta cctgtcgggc gctaccggag cccgggagtt
      961 cgagagcggc gcgctctatg gcgtggagat ccccgagacc cagcgcctgc tagactggga
     1021 cattcccctg gcatcgcagc ccgaggagat caagcagccc ctgagccagg tgtggagcga
     1081 cctctacctc gcatcggcca cgggccagga cctgagcgcg atcgcccgca tggatccagt
     1141 ccagcgcagg ctggccaagg agcgcctcca ggtgccgccc cttcccgagg gcacgaccgg
     1201 ggagcagctc tacctgcagc tcgccgagca gctcgggggc aagggccagg ccagcgacta
     1261 cctgaacagc aagggcatcc ccggcaccac ctacatcggg gtggagagcc aggagcgcaa
     1321 cgcggtgctc ttcaacgacg acgccaccaa cgtattctcg aaggacggtc agccgttcgg
     1381 caaggcagag cttccggttc ccgaggtcga ggagctggac atgggcgcct tccgggatat
     1441 gttctccggt cgggcgccgc aagtcgcagc gggcgctggg gccgctggtc tcctggcatc
     1501 ccagggcgca tcggctgagg ctctccaggc tgacgcgcag cgacgtgctg ccgaggcata
     1561 cgacccggtc gcggcgcttc gagcgcagcc cacgtaccag cagcccgcca ccgacggcct
     1621 cacccagacg ctccagggga gcgcggagca gattgccggg caagagcttg ccgccaaccg
     1681 caaacgcgcc caggagcgcg ctggcgggct cgtggaggcc ggcactggcc tgctgggtga
     1741 catcgggcag gcaatcccca agaacatccg cgagaacccc gtggcgagcc tggctggtga
     1801 gttcaccggc ctccctggtc tgtatcgggc cggggaggag tacctgagcg gggtggagcg
     1861 catcagcccc gaggcggcga tcgacgttgc cagcgcggcg gtgccgttcc tgccggtagc
     1921 tcgaggcctt cgtgcggccg atcgtgcggt cacggcagcg gacgaggcgg gcagggcctc
     1981 tgtggcgggg acctcccttc gccctggcac cgagggctac cgcggagcga ccgccagcct
     2041 ggaccaagcc cgccgggtgg acgagcccag ggtggagatc ccgaggctcc ggtcggccaa
     2101 caagggccgc cccaagggca cgatcgtcgg tctgcccaag ggcctggagc ccaccgaggc
     2161 caacgtcgag cgcctggccg acgagtacgc cggccgggta atgaagggca tcgagaacgg
     2221 cgtgcagccc gggtacttct acaaggaagg ctcccagaac gtgcgggatt ggaccggaaa
     2281 cgaccccta
//
LOCUS       DBNHOX010000191         3070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_850949, whole genome shotgun sequence.
ACCESSION   DBNHOX010000191 DBNHOX010000000
VERSION     DBNHOX010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_850949"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="AC16UZ_04145"
     CDS             complement(<1..621)
                     /locus_tag="AC16UZ_04145"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HIR6245906.1"
                     /translation="MASDETAGAVSPPLLLVDGSSYLFRAYHALPELKTSTGLHTGAV
                     RGVIGMLRRLAKDYRGSPIAVVFDAKGKTFRDDIYVQYKANRPPMPDDLSEQIEPIHQ
                     IIQAMGLPLIVVPGVEADDVIGTLAAEATRKRRDTVISTSDKDMAQLVTEHVTLVNTM
                     SETTLDPEGVVDKFGVPPELIIDYLALMGDSVDNIPGVPKVGPKTAA"
     gene            746..1006
                     /locus_tag="AC16UZ_04150"
     CDS             746..1006
                     /locus_tag="AC16UZ_04150"
                     /inference="COORDINATES: protein motif:HMM:NF022639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HIR6245907.1"
                     /translation="MAAAATLPPTALASEAQDGLQGPDITIIAQEDKTILEFRQAGQL
                     RMVKIVPRLGKPYYLVPRDSTTGFGDLERADMLLPSWVIVEF"
     gene            1037..2002
                     /locus_tag="AC16UZ_04155"
     CDS             1037..2002
                     /locus_tag="AC16UZ_04155"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR6245908.1"
                     /translation="MNAITEPGHPGIEETLTRYGVGELISAWPGSAASRCAYRVRTRR
                     AGREHDYLLLRVQAPRFEAELAALEACDRAGLPVAKAIPNLDGQPVDTLNGETVILTR
                     RLPGRHVYNPTVRQVAALGRFMARFHEAGGNLGPLPAWAWDQRWLAEASAALDGRLSY
                     PSARLMEATVQQVVSMLDREDVAGLPAGAIHGDICRDNVLFNEHGLSGVGGFSQVSSG
                     FLVYDLAVAANDWCIDANGMLDPERTLGFLRAYQKLRPLVRAELWFFSGFTLYAALNG
                     WIKRLAFAQGRQVSPGLHFNTPLELEKVVEQHAAHFFYLDERLLT"
     gene            complement(2139..2786)
                     /locus_tag="AC16UZ_04160"
     CDS             complement(2139..2786)
                     /locus_tag="AC16UZ_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR6245909.1"
                     /translation="MRSVTATVLLCLSLAAAAVGAFVYSVLREPVLSDAELRDLGVFV
                     LPRPRAIGAFQLESTRGGSFTAADLQGGWSFLFFGFTNCPDVCPTTMAVLAQAERELL
                     AGSPEDADRFAGVLVSVDPERDDVRTLGEYTAAFSPRFFGVTGPFDELASFARQLNVG
                     FGKVPAPEGSFSEYLVDHGAQIVIINPQGHYHGFIRAPHTADTIVQTFASLAARF"
     gene            complement(2783..>3070)
                     /locus_tag="AC16UZ_04165"
     CDS             complement(2783..>3070)
                     /locus_tag="AC16UZ_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245910.1"
                     /translation="GERYTRLHILYYTLILVAVSLLPFVIGMSGWLYLAGATALGGGF
                     IYWAVVLLRNRNPRAPLETFRYSIVYLGLLFVALLADHYVFADHLVGSSLQ"
BASE COUNT          564 a          987 c         1009 g          510 t
ORIGIN      
        1 tgccgccgtc ttggggccca ccttcggcac cccgggaatg ttgtccacgc tgtcccccat
       61 cagcgccagg tagtcgatga tcaactccgg cggcacgccg aacttgtcga ccacgccctc
      121 cgggtccagg gtggtctcgc tcatggtgtt gaccagggtc acgtgctcgg tcaccagctg
      181 ggccatgtcc ttgtcgctgg tcgaaatgac ggtgtcgcgc cgcttcctgg tggcctccgc
      241 agcaagggtg ccgatcacgt cgtctgcctc gacgcccggg accacgatca gcggcaggcc
      301 catggcctgg atgatctgat gaatcggctc gatctgctca ctcagatcat cgggcatggg
      361 cggccggttg gccttgtact ggacataaat gtcgtcccga aacgttttgc ccttggcgtc
      421 gaagaccacg gcgatggggc ttccccggta atccttggcg agccggcgca gcatgccgat
      481 gacgccgcga acggcgccgg tatgcaaccc ggtgctcgtt ttcaactccg gcagcgcgtg
      541 gtacgcccgg aacaggtagg acgatccgtc cacgagaaga agcggcgggc ttaccgcccc
      601 ggcggtttcg tcgctggcca tgtgtcgacc ttttttatga tgcggatgct ttgccttgca
      661 cagccgtttc ggtcaacatc cccctgtgca acgctcgctc cggacactat cacagacggg
      721 ccgcaaaggc gcagcagtgc tggcgatggc ggctgccgcg accctgccgc ccaccgcgct
      781 ggcaagcgaa gcgcaggacg ggctgcaggg cccggacatc accatcatcg cgcaggagga
      841 caaaaccatc ctggagtttc gccaggcggg acagctcaga atggtcaaga tcgttccccg
      901 cctgggaaaa ccctactacc tggttccaag agactcgacg acgggcttcg gcgatcttga
      961 gcgcgccgac atgctgctgc ccagctgggt catcgtcgag ttctgatacg gccatgctgg
     1021 acagctggaa tagccaatga acgccatcac cgagcccggc cacccgggaa tcgaagagac
     1081 cctgacgcgc tacggggtcg gcgagctcat ctcggcctgg ccaggctcag ctgccagccg
     1141 ttgcgcttat cgcgtcagaa cccggcgggc cggaagggag cacgactacc tgttattgcg
     1201 ggtgcaggcg ccccgattcg aggccgagct cgccgctttg gaagcctgcg accgggcagg
     1261 ccttccggtg gcaaaggcga tcccgaatct ggatgggcaa cccgtggaca cgttgaacgg
     1321 cgaaacggtg atcctcaccc ggcgcttgcc cggccgccac gtttacaacc ctaccgtccg
     1381 gcaggtagcg gcattgggcc ggttcatggc tcgttttcac gaagcgggcg gcaacctcgg
     1441 accgctgccg gcatgggcct gggaccagag gtggctcgcc gaggcgtcgg ccgctctcga
     1501 cggccggctc tcgtatccga gcgcgaggct catggaagca accgtccagc aggtagtctc
     1561 catgctggac cgggaagacg tggcggggct gcctgccggg gccattcacg gtgacatctg
     1621 ccgtgacaac gtgctgttca acgagcacgg gctgagcggc gtgggcgggt tcagccaggt
     1681 gtccagcggg ttcctcgtct acgatctggc ggttgccgcc aacgactggt gcattgacgc
     1741 caacggcatg ctcgaccccg agagaacgct gggcttcctt cgggcgtatc agaaactgcg
     1801 ccccctcgtt cgagccgagc tctggttctt ctcgggtttt accctgtacg cggctttgaa
     1861 cggctggatc aagcgcctgg cgttcgctca gggccgtcag gtgtccccag ggctgcactt
     1921 caacacgccg ttggagctcg agaaagtcgt cgagcagcac gccgcgcact ttttctacct
     1981 ggacgaacgg ctgctcacct gaagccgttt cgggcttgcc ggctgggctc accgggtgag
     2041 cttaccggcc gggcgtaaca agccggccga tcacaccggc cccggacgga cagagcggcg
     2101 gaaaccggcc gccagccggc gacgcgggca ggccgcgctc agaaccgcgc ggccagtgac
     2161 gcaaaggtct gcacgatcgt atccgccgtg tggggcgcgc ggatgaagcc gtgatagtgg
     2221 ccctgcgggt tgatgatgac gatctgggcg ccgtgatcga ccagatactc gctgaaagac
     2281 ccttctggag cgggaacttt gccgaatccc acgttcagct gccgggcaaa cgaggcgagc
     2341 tcgtcgaaag gtccggtcac accaaagaag cggggggaga aagcggccgt gtactcaccc
     2401 agcgtcctca cgtcgtccct ttccgggtca accgaaacca gcaccccggc gaagcggtcg
     2461 gcatcctccg gtgagcctgc aagcagctcc cgctcagcct gggccagcac ggccatcgtg
     2521 gttgggcaca cgtcgggaca attggtgaag ccgaaaaaga ggaacgacca accgccctga
     2581 aggtcggcgg cggtgaagga accgccccgg gtgctctcca gctgaaacgc gccaatggcg
     2641 cgcgggcgag gcagcacgaa cacaccgaga tcgcgaagct ccgcatcgct gagtaccggc
     2701 tcgcgcagga cgctgtacac gaaggcgcct acggcggctg cggccagcga caggcacagc
     2761 agcaccgtcg ctgtaacgga tctcactgca ggctcgatcc caccaggtgg tcggcgaaga
     2821 cgtagtgatc ggcgagcagg gcgacgaaga gcaggccgag gtagacgatg gagtagcgga
     2881 aggtttccag cggtgcccgc gggttgcggt tgcgaagcag gacgacggcc cagtagatga
     2941 acccgccacc cagggcagtg gccccagcca ggtatagcca gccgctcatt cctatcacga
     3001 acggaagcag gctgacggcg accagaatca gggtgtaata gaggatgtgc agcctcgtgt
     3061 agcgttcccc
//
LOCUS       DBNHOX010000192         5072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1622860, whole genome shotgun sequence.
ACCESSION   DBNHOX010000192 DBNHOX010000000
VERSION     DBNHOX010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1622860"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="AC16UZ_04170"
     CDS             <1..669
                     /locus_tag="AC16UZ_04170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6245911.1"
                     /translation="RTAEALKPYYPYGCKRPTFHDEYLPTFNLPHVTLVDTAPLGVTR
                     INERGVVHDGREYPLDVLIYATGFQWMATSTFNMIRGRGGRTLNEKWQEEGTRTFLGI
                     HSHGFPNLFIVSGPQGGGGSFNFTDAIDTHADYICWMLETMRDRGARTVDVRKEPEDE
                     YAEHCRAADVMTAPLRDCISYYNGHGDAEPGSLAYYGGGRWHKYRVRAQESLEPYDFD
                     DIPE"
     gene            769..2265
                     /locus_tag="AC16UZ_04175"
     CDS             769..2265
                     /locus_tag="AC16UZ_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6245912.1"
                     /translation="MTDLVRKGWTVLALWALTMILGYFGLGGFLAETVEGIGGVPAWP
                     TALFVVLLAVSLWASVVWARGLKAKPEAGAALEERPLASGRRRFLTGTAAVTGGMLGA
                     AGATIGRVAPWATVTLPALRQPVTVTDPNPKDDWLGARVRSYRPLGDTGLMVSDIATG
                     STRIHHNSDPVGYLKSLLDRGVNYIDASPDYAPESEGIIAEAIVGRPRDQLVIASKFC
                     TRDGHLRQGSSVAAYMTAIDGTLSRMGTDYIDVAHIHSCDSTERLMDENVHEAFDRLK
                     DAGKVRHLGVSTHTPNLEEVADTAIDSGRFKVMMLAYHHGAWPKQMDIIDRAAAAGMG
                     IVAMKTLKGAKHRGLAEFRPEADSYAQAAFKWVLANPSVSNLVVSFYDNQHPDEYLFA
                     SGKSLERSDIAVLGKYDQLIAGTHCFQHCGDCLGACPSGLAINDILRHRMYFEDYGDE
                     KQAMQLYAKLDRQADVCIDCSAPCLGSCPQGVEIQERMIGAHEKLRWA"
     gene            complement(2297..3499)
                     /locus_tag="AC16UZ_04180"
     CDS             complement(2297..3499)
                     /locus_tag="AC16UZ_04180"
                     /inference="COORDINATES: protein motif:HMM:NF017984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6245913.1"
                     /translation="MPTVTDISTRDARRLFLHRQGLLRREPFGRGGQAVARAVERLGW
                     VQIDTISVVERAHHHVLRSRVAGYQPRWLDDLQHRERAVFEYWSHAAAYLPMRAYRFY
                     LPMMRWSRRGDAADPKMAREVLRRVRDEGPLQSRDFEAAGHRSDGWWDWKPAKRTLEQ
                     LFHRGELMVTRREGFQKVYDLPERVLPESVDTSFPDASEWGRFVVGMMIDALGIASDK
                     EIAYQRIAAPRLYSRPILPLVQQAAADMVEAGELEQVTVEGGGSYLTRHLDWLPVRLG
                     RKRVTILSPFDNAVIMRERARELFGFDYAIECYLPAGKRRYGYFSLPILFGDELVGRM
                     DSKADRRTGTLTVNLLHLEPGADPAAIRDPLMQALNGFARDNGCTRVIMGATEPASLK
                     AELARVLG"
     gene            complement(4098..>5072)
                     /locus_tag="AC16UZ_04185"
     CDS             complement(4098..>5072)
                     /locus_tag="AC16UZ_04185"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6245914.1"
                     /translation="ELTYELETVARNDFFGTLPGAFTAHPKVDPQTGELHGMAYAWPQ
                     WMDHVQYVAVAPDGRVRRTVDVKLPGMTMMHDFSLTQRYVVIYDQPVTVDFAMVGNTS
                     FPMRWNPDYGNRLGLLPREGDADEIIWIDAPLGYCFHPMNAYDDPDGSVIVDLCVYER
                     MFDAYPRDPFGDSMASLQRWRVNPANRSISVDVIDASANEFPRHRGSLSTLPYRYGYC
                     ASPSSDMSAGWPTLKHDLQTGERSVFDHGPGRAAGEPVFVAREGSAAEDDGYLITYVH
                     DLAQSSAEFVVMDAQDFDRGYLARVALPQRVPFGFHGNWVSDRAVSPG"
BASE COUNT          881 a         1651 c         1695 g          845 t
ORIGIN      
        1 aggacggccg aggcgctgaa accctactac ccttacgggt gcaagcggcc gacgttccat
       61 gacgagtacc tgcccacctt caacctgcct cacgtcaccc tggtggacac cgcgccgctg
      121 ggggtcacca ggatcaacga gcgtggcgtc gtgcacgacg gccgggagta ccccctggac
      181 gtgctgatct acgccaccgg cttccagtgg atggcgacgt ccacgttcaa catgatccgt
      241 ggacggggtg ggcgcacgct caacgagaag tggcaggagg aggggacccg cacctttctc
      301 gggatacaca gtcatggctt tccgaacctc ttcatcgtct cgggtcccca gggcggcggc
      361 ggcagcttca acttcaccga tgccatcgac acccacgccg actacatctg ctggatgctg
      421 gagaccatgc gcgaccgggg cgcccgtacg gtagacgtgc gcaaggaacc ggaagacgag
      481 tatgccgagc actgtcgggc ggcggacgtc atgacggccc ccctgcgcga ctgcatttcc
      541 tactacaacg gtcacgggga cgcggagcct ggcagccttg cctattacgg cggcggccgc
      601 tggcacaagt atcgcgttcg cgctcaggag agtctggaac cctacgattt cgacgatatt
      661 ccggagtagt ggaaacccct ggggatcggc gagtactgca acggacgcgg tttgatcccc
      721 ccgcggctgt cgatatagtt cagggatagc cggcctggag aggggatgat gaccgatctc
      781 gttcgcaaag gatggaccgt tctggccctt tgggccttga ccatgattct cggctacttc
      841 ggtctcggcg gctttctcgc cgagacggtt gaaggtatcg gtggggtacc cgcttggccc
      901 acggcgctat tcgtggtcct cttggccgtg agtctctggg ccagcgtggt gtgggcgcgc
      961 gggctgaaag cgaagcccga agcgggggcc gcgctggaag aaaggccact cgccagcggg
     1021 cgacgtcgtt ttctcacggg taccgcggcg gtgaccggcg gcatgctcgg ggccgcgggg
     1081 gcgaccatcg gcagggtggc accctgggcg acggtgacgc ttcctgccct gcgccagccc
     1141 gtgaccgtca cggatccgaa cccgaaggac gactggctgg gcgccagggt gcgcagctac
     1201 cggcctttgg gagacaccgg attgatggtc tccgacattg ccaccggctc cacgcgcatt
     1261 caccacaact cggatccggt cgggtacctg aagagccttc tggaccgcgg ggtcaactac
     1321 atcgacgcct cacccgacta cgccccggag agcgagggca tcatcgccga ggccattgtc
     1381 gggcggcccc gggaccagct ggtcatcgcc tccaagttct gcactcgcga cggccatctc
     1441 cggcagggct cgagcgtcgc agcttacatg accgccatcg acgggacgct cagccggatg
     1501 ggcacggact acatcgacgt cgcccacatc cattcctgcg acagcaccga aaggctcatg
     1561 gacgagaacg tccacgaggc tttcgatcgc ctcaaggatg ccggcaaggt gcgccatctg
     1621 ggagtgtcta cccacacgcc caacttagag gaggtcgccg acaccgccat cgacagcggc
     1681 cgtttcaagg tgatgatgct ggcctaccac cacggcgcct ggccgaagca gatggacatc
     1741 atcgatcgcg cggccgcggc gggcatgggc atcgtcgcca tgaagacgct caagggcgcc
     1801 aagcaccgcg gccttgcgga attcaggccc gaagcggact cctacgccca ggcggccttc
     1861 aagtgggtgc tggccaaccc gtccgtttcc aacctggtgg tttcgtttta cgacaaccag
     1921 catccggacg agtacctgtt cgcctcgggc aagtccctcg agcggagcga catcgcggtg
     1981 ctcggcaaat acgaccagct cattgcgggc acccactgct tccagcattg cggcgattgc
     2041 ctcggggcgt gtccgagtgg gctcgccatc aacgacatcc tgcgtcatcg catgtacttc
     2101 gaggattacg gggacgaaaa gcaggccatg cagctttacg ccaagctgga ccggcaggcg
     2161 gacgtctgca tcgactgctc agcaccctgc ctcggcagct gtccccaggg ggtcgagatt
     2221 caggagcgca tgatcggcgc ccacgagaag ctgcgctggg cctgagacct tgtagccgat
     2281 tgggccttcg ccccggtcag ccgaggacgc gagcaagctc ggctttcagc gatgcgggtt
     2341 cggttgcgcc catgatgacc cgggtgcagc cgttgtcccg ggcaaacccg ttcagtgcct
     2401 gcatcaaggg gtctcggatc gcagcgggat ccgcgccggg ttcgagatgg agcaggttca
     2461 ccgtcagggt gcccgtgcgc cgatccgcct tgctgtccat ccgcccgacg agctcgtcgc
     2521 caaacaggat cggcaggctg aaatagccat agcgccgctt gcccgcgggc aggtagcact
     2581 cgatggcgta gtcgaagccg aacagttccc gggcacgctc ccgcatgatc acggcgttgt
     2641 cgaacggcga gaggatcgtc acgcgtttgc ggcccagccg caccggcagc cagtccaggt
     2701 gacgcgtcag gtacgagccg ccgccctcga ccgtgacctg ctccagctcc cccgcttcca
     2761 ccatgtcggc ggccgcctgc tgcactaacg gcaggatcgg gcgcgagtag agcctgggcg
     2821 cggcaatgcg ctgataggcg atctccttgt cgctggcgat gcccagggca tcgatcatca
     2881 tgcccaccac gaagcggccc cactcggaag catcgggaaa cgacgtgtcc acgctctcgg
     2941 gcagcacccg ctcgggcaga tcgtagacct tctgaaaccc ctcgcgccgg gtcaccatca
     3001 gctcgccccg gtgaaagagc tgctcgagag tgcgcttcgc cggcttccag tcccaccagc
     3061 cgtcgctgcg atgaccggcc gcctcgaagt cccgggactg caggggaccc tcgtcgcgca
     3121 cgcgtcgcag cacttcccgg gccatcttcg gatccgcggc gtccccgcgc cgcgaccagc
     3181 gcatcatggg caggtagaag cggtaggcgc gcatgggcag ataggcggcc gcgtgggacc
     3241 agtactcgaa caccgcccgt tctctgtgct gcaggtcgtc gagccacctg ggttgatacc
     3301 cggccacgcg gctgcgcagc acgtggtgat gggcacgctc caccaccgag atggtgtcga
     3361 tctgcaccca gcccaggcgc tcgacggcgc gggccacggc ctgacccccg cggccgaacg
     3421 gctcgcgccg cagcagtccc tgccggtgca ggaacaggcg gcgggcgtcg cgggtggaaa
     3481 tgtcggtgac ggtcggcatg tgggctcagg cgcctgcggg tcttgcgtcg acgccgcgag
     3541 catagcggct cgctcgacct gatggccatg gcggccgggt ggatcgaggc gcatgttgcg
     3601 ctgaggacga cccgaaacgc gagagcctca ccgccatcgt gctcggggcc cgccatctcg
     3661 ggaatctcat tcaggacgtg ctggatctgt cgaggataga aagcgatcac cgtgatcgat
     3721 acggggatag gcgtcccaga ggcattcaga gacggcctct ttgagcagtt tgcgcgggct
     3781 gacgccacga tgaccaggcg ctagggtgcg ccggtccggg gcttttcatc agccagcggc
     3841 tggcttcgct gctgggaggc agggtcgaat acgcgccggg ggagaccggc agtgttttcc
     3901 gtctggtgct ggcgatctgc agggcgacgt ccttcgccac aagatgggtt ctgcagagat
     3961 gaatccgttc acggacatgc tcccgaagcc catcgacatt cagcgattgc agtcgctgct
     4021 gacgcgattg ctgagatctc gcgacgccgt caaagctgcc gatccctgac cgggctcgaa
     4081 atccccagga gctgccgtca accgggcgat acggcgcgat cgctcaccca gttgccgtga
     4141 aagccgaagg gcacccgctg cggcagcgcc acccgggcca gatagccgcg atcgaagtcc
     4201 tgggcgtcca tcaccacgaa ctccgccgag ctttgtgcga gatcgtggac gtaggtgatc
     4261 aggtagccgt cgtcctctgc cgccgagccc tcccgggcga cgaacacggg ctcgccggcc
     4321 gcccggccgg gcccgtgatc gaagacgctg cgctcgcccg tttgcagatc gtgcttgagc
     4381 gtcggccagc cggcggacat gtcggaagac ggcgatgcgc agtagccgta gcgatagggc
     4441 agggtcgaaa ggctgccgcg gtggcgcgga aactcgttgg ctgaggcgtc tatcacgtcg
     4501 acgctgatcg accggttggc gggattcacg cgccagcgct gaagagaggc catgctgtcg
     4561 ccgaaggggt cgcgagggta cgcgtcgaac atacgctcgt agacgcagag gtcgacgatc
     4621 accgagccgt cggggtcatc gtaggcgttc atcgggtgga agcagtagcc gagcggcgcg
     4681 tcgatccaga tgatctcgtc ggcgtcgcct tctctcggca gcaggcccag gcggttaccg
     4741 tagtccggat tccagcgcat ggggaagctc gtgttgccca ccatggcgaa gtccacggtc
     4801 acgggctggt cgtagatgac gacgtaacgc tgggtgagcg agaagtcgtg catcatcgtc
     4861 atgccgggca gcttaacgtc caccgtgcgg cggacccgcc cgtcgggcgc cacggcgacg
     4921 tactgcacgt ggtccatcca ctggggccag gcgtaggcca tgccgtgcag ctcgccggtc
     4981 tgggggtcta ccttcgggtg ggccgtgaac gcgccgggca gggtaccgaa aaagtcgttg
     5041 cgcgccacgg tttccagctc gtaggtgagc tc
//
LOCUS       DBNHOX010000193        13959 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_787147, whole genome shotgun sequence.
ACCESSION   DBNHOX010000193 DBNHOX010000000
VERSION     DBNHOX010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13959
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_787147"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="AC16UZ_04190"
     CDS             <1..210
                     /locus_tag="AC16UZ_04190"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6245915.1"
                     /translation="AIEILRKELGATKVLCFGDNNNDLSMFEVADESYAPSNAQPEIL
                     EAATAVIGHHDEDGIARFLRERFSL"
     gene            251..1240
                     /locus_tag="AC16UZ_04195"
     CDS             251..1240
                     /locus_tag="AC16UZ_04195"
                     /inference="COORDINATES: protein motif:HMM:NF021778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vgb family protein"
                     /protein_id="HIR6245916.1"
                     /translation="MTRSFHGLILVLIAAVSSAIEAAAEPTGTLLVVNKGGDSVTMLD
                     VSDGRELAELPTGRGPHELVVSPDAKLAVVTDYAGGNSLTVIDIAGRRVVRTVDLEDY
                     PRPHGIAFMPDGDTVVVTSEATDQIVFVRISDGAVVGSIETGGAGSHMLAITANGSRV
                     FTSNIQSDTVSELDVASGKQVRAYPTPKQPEAIAVTADGSEIWVGSNAEGSLSVIRTD
                     DATIETIAEGFGWPYRILISRDGRLVLVPDLKRNVLRMFDRTNRVELASVELADAAPQ
                     GLVFHPEERWLFVSLNGQNQVAVIDLSRSAIVRHIDTGPRPDGIGYAATKGQP"
     gene            1237..1875
                     /locus_tag="AC16UZ_04200"
     CDS             1237..1875
                     /locus_tag="AC16UZ_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation efflux transmembrane domain-containing
                     protein"
                     /protein_id="HIR6245917.1"
                     /translation="MSDDCCANGIDASGLETRHRRALASVLAINVAAFLVMIVGAVLS
                     GSSSLLSGTLDNLGDALTYALSLAVIGSTASAKARVAFFKGLLIFAAALGVAAQIGWR
                     LSNMETPVFETMGVAATLNLAANLICLRILFPFRNDDLNMTSVWECSRNDIFDGLAVL
                     TATVAVWVSDSAWPDLLIAIALLIVFLRSAARILSRSWRELHAAPTPGPGTG"
     gene            complement(1909..3012)
                     /locus_tag="AC16UZ_04205"
     CDS             complement(1909..3012)
                     /locus_tag="AC16UZ_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6245918.1"
                     /translation="MAGIPETPEDFTTSWLCEVLGVPESEVLDVDVRTSEADSGFVSL
                     ACFLELETRPGSGLPRQLVGKLAPTFEGARKLSREFGLFAREKQFYSEIAPLVAIRSP
                     GAYHCDYDAELGTGVLLLEDCSHMRAPVQTDEQPTTLAELELIVDAAADMAISSWNAD
                     WLATKDELMGPDHPAFVGYFSGIQEGYKDFVDSEFMSWAPDGFEGLAVRLGENMRTIA
                     AAQPETNLSLCHMDLRLANVFLDEESDDPVVLYDWQSMYPGRTAQDLGYLITSGYTPA
                     FRREHERNLVSRYHQRLVDAGIPGYSFDEAWFDYRHGIIIGLRLLPMALGDLDVSTEG
                     GQAIFRKIIRNFPEAALDHGGVELVDEILARRA"
     gene            3162..3941
                     /locus_tag="AC16UZ_04210"
     CDS             3162..3941
                     /locus_tag="AC16UZ_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245919.1"
                     /translation="MKTINRTLGRSLLVLIPFLALNGMFAYADSHGPALPSGVLEVFQ
                     CNYNSGKDRDDLNALRDFYLKQAKKVNYTPPDAFLWTMTNGSWPTTLTWVNVHEDLSA
                     YSRSYDTIAASTDLAAVNERAETVMTCVPALGSYAHAFIRDPDAQGPWEATVASYACD
                     LRPEQDMSDLEDLMEHVATVNQSMGDAAMDELLHFSPMTGLNNQPDVLVFAASRRGAS
                     SWSSHLALLEQSGAGRAVLAHFDSVLDCRLSLWDVEQMVGG"
     gene            complement(3962..5356)
                     /locus_tag="AC16UZ_04215"
                     /pseudo
     CDS             complement(3962..5356)
                     /locus_tag="AC16UZ_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
     gene            complement(5375..6448)
                     /locus_tag="AC16UZ_04220"
     CDS             complement(5375..6448)
                     /locus_tag="AC16UZ_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245920.1"
                     /translation="MEAGNHDLLRLRGALNATVADRLALRLAGLSLDRDGYTDNLAGG
                     QVPGVASEIDGRDLYSVRASATWRVTEATEIWGLYERFDEDDDRMLTHNRRCKTALRP
                     TTRTGCEPGEWGLEPNNPNQARFQTSITNGLRGVIPLGARDAETGLTFAYPRPEITNL
                     RDVHIDFAPVYEFEQNLWQLGASHDFDWATLSFIGSYQDWRLRTLQDNDWSVGHELAP
                     IPENPSGLYPVSALPEGKNPLDGPECNVNEAQFGVLGGCVLDPNVTRQFWYGERREWR
                     DFYSAELRLRSDLDGRVNFLIGGNYQFSDDHAVSGGGTNDYWEMWTRFGRAQAPAPGP
                     NPFALNLNALDRLMVLAGGEDKN"
     gene            complement(6403..6939)
                     /locus_tag="AC16UZ_04225"
     CDS             complement(6403..6939)
                     /locus_tag="AC16UZ_04225"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6245921.1"
                     /translation="MARGIPISAMVLMMPCALQAQERLVIEEVIVTAQRVEESAQKVP
                     IALNAFDEMGIEDRQIIGIGDLSLFVPNLSYNTNNVGDTHVTIRGVGSLVSRRDSESG
                     VSLHVNQVPLPSGQPPFEIYDLARIEVLRGPRARCTAATPRAVSSTSLPTGRALTVQA
                     GTWTWRPVTTTSCGCAAR"
     gene            complement(7103..7396)
                     /locus_tag="AC16UZ_04230"
     CDS             complement(7103..7396)
                     /locus_tag="AC16UZ_04230"
                     /inference="COORDINATES: protein motif:HMM:NF020873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TehB family protein"
                     /protein_id="HIR6245922.1"
                     /translation="MAFTLHMRALPENVLPYRRTATFHQDSIPPGLLKAHSTKPGTWG
                     RIVVVTGELLYRILEPDPAEFLLSPHIMGVVEPTVLHEVAPVGEVEFYIEFLR"
     gene            complement(7483..8250)
                     /locus_tag="AC16UZ_04235"
     CDS             complement(7483..8250)
                     /locus_tag="AC16UZ_04235"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF048554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6245923.1"
                     /translation="MDLGLNGAKAVISGGTQGIGLAVAKQLIVEGVAVSIAARRQEGV
                     DAALEELSALGTVVGQVCDVADYDATVAWMQNAADALGGIDIVVHNATASGQPGPGPQ
                     SWINNFSVDVLGMVGMSEGALPFLEQSERAALVQIASITGIEHHDVPISPSYGAMKAA
                     SIRHMAQLAQQWGPKGIRANTVAPGPIFIENGVWNGIKDNAPALYERDRDYHPTKRMG
                     TGEEVARVVAFLASPAASWVNGTTVRVDGAFTRGVNF"
     gene            complement(8377..8904)
                     /locus_tag="AC16UZ_04240"
     CDS             complement(8377..8904)
                     /locus_tag="AC16UZ_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6245924.1"
                     /translation="MSKEFNYDDVSTYTLDKADELALIAAQNECTFMWSTKDGWPVGV
                     IMTYVYAEDCFWLSVSSLRVRVKAVQRDPRASISITSKGTPLRSGQSLTYKGTCEVFS
                     DQATVDWFLPELAKALRIGDERARRVFVELNNTENRRVIKFTPTKAIGFDGRKMGKAT
                     MEATGGDPVVTKRSS"
     gene            9098..10447
                     /locus_tag="AC16UZ_04245"
     CDS             9098..10447
                     /locus_tag="AC16UZ_04245"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6245925.1"
                     /translation="MAAVIEEKPSHLRGNGRPITEERTLTDLKVTGAIPPELDGRYVR
                     NGPNPLSGTSAHPFFGDGMLHGVHLRDGKAQWYHNRYVQTPFISNPSVDILDASVMLD
                     MRASKANTHVVGHAGRILALEEGHFPYEVDADLNTVGPVDFDGVLTGSFTAHPKICPV
                     TGEMLAFGYSAFEPYLRYLRVSAEGKLVQTENITVGGPTMMHDFNITKNFVIFMDLPA
                     VFDMSLAMSGDMPIRWDDDYPARLGVMPRTGTDAQVTWYDINPCYVFHPMNSYEDGDT
                     IVLDVARFDHIWRESAMDFAPPQLWRWTIDTATGRVREEQIDDRPAEFPRVADSLIGL
                     RHRYGYMMAMATGDQGDPASQSGAILKYDRETGSRSEIELGRGHTCGEPVFAPAADAK
                     AEDDGYLMTYVYDAETDASRFVIMDAATMDDTPVASIELPRIPNGFHGSWIPSSVAG"
     gene            complement(10537..11289)
                     /locus_tag="AC16UZ_04250"
     CDS             complement(10537..11289)
                     /locus_tag="AC16UZ_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6245926.1"
                     /translation="MNKVKDRIALVTAAADGIGASTATLLAEEGAVVVVTDINGEGAV
                     KLADEFGGMGLEHDAGSEADWKRVINEVTDRYGRLDILVNNAGIGAGIGDIEHQTLDD
                     YRAVMRVCVDSVFIGSKLAIELMKRDGGAIVNVSSVHGIRAAAHEAAYSAGKGAVRLL
                     TKSIALYCAQCGYRIRANSVHPGYILTTMMKEWMERSGNSADIMAGLVQQHPIGFLGE
                     PRDVANGILYLASDDARFVTGSELVIDGGFML"
     gene            11417..11947
                     /locus_tag="AC16UZ_04255"
     CDS             11417..11947
                     /locus_tag="AC16UZ_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6245927.1"
                     /translation="MNQEDQMRPDAESRYHALPGGINGKEHAVNLLEKGSLRWKRFTG
                     GDKFDYPIDYSAALLNAREDGHVDLLYRWEPNCYCHFHRHTAETTSTVLEGELHVIDI
                     DLETGKEVGRRVRRAGDYAHKAPGDVHMERAGPEGALVLFSLYAPDGLLAESLAEDGT
                     VLGVSTMDRILKRRAS"
     gene            12018..12884
                     /locus_tag="AC16UZ_04260"
     CDS             12018..12884
                     /locus_tag="AC16UZ_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015016 - heparan sulfate
                     N-sulfotransferase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HIR6245928.1"
                     /translation="MRVYKDAVMDNARWEGFEPRPGDIFVCTPSKCGTTWMQMIVANL
                     LWPDGEFPGSIVNGICPWIEAKFTPAEKMHAMLRAQTHRRAMKSHTPADGIPWFDDAR
                     YITVVRDGRDAFMSWCNHVSRMMVTDMLNEKASEDGLPAINTFDGVDYRGYFHEWLER
                     NNFFDVVASYWAVRDRPNLLFVHFNDLKADLTGQVRRIAPFLDIELDEDQWPAVVKRC
                     TFEGMRKADKKVGDFEGFFEGGLEGFVYKGTNGRWRDVLNDDDIAAYRRKVAETLPPD
                     AARWVEFGARAT"
     gene            12989..13606
                     /locus_tag="AC16UZ_04265"
     CDS             12989..13606
                     /locus_tag="AC16UZ_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245929.1"
                     /translation="MSIRARGRLLIAALSGLLLPAAYAVTIDIAEVRDEMAFVKGSDA
                     GRKQQILWEGAPATTANGKGNFSFEGPLPNPCVPEDCSGTLTAGDHVEPVTLTFPPAE
                     PPVQTDEALVQVAAQADAHNGEALRTVGFVDDGLTIKVVSGGADTNLRYWTNELHELE
                     IGHDLPNIIYDLEVSNDYSAVVTGEGGWNGSAGASTTSPFLRKAT"
     gene            13549..13674
                     /locus_tag="AC16UZ_04270"
     CDS             13549..13674
                     /locus_tag="AC16UZ_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HIR6245930.1"
                     /translation="MERKRGRVNDVAFSPEGDLIVSGGADGTLKLWAYPDPPPPD"
BASE COUNT         2625 a         4493 c         4319 g         2522 t
ORIGIN      
        1 gccatcgaga tcctgcgcaa ggagctcggc gcgaccaagg tgttgtgctt cggcgacaac
       61 aacaacgacc tcagcatgtt cgaggtcgca gacgaaagct acgcgcccag caacgcccag
      121 cccgagattc tggaggcggc gaccgccgtg atcggccatc acgacgagga cggcatcgcg
      181 cgatttctcc gcgagcggtt ctcgctgtag cagaggacgg acgccgcacc ccgatgagca
      241 gggagacagg atgaccagaa gtttccacgg cctgatcctc gtgctcatcg ccgcggtgtc
      301 ttcagccatc gaggcggcgg ccgagccgac gggaacgctc ctcgtcgtca acaaaggagg
      361 cgacagcgta acgatgctgg atgtgtccga tggccgcgag ctcgcggagc ttcccacagg
      421 ccggggaccg cacgagctcg tggtttcgcc ggacgcgaag ctggctgtgg tgacggacta
      481 cgccggcggc aactcgctca ccgtcatcga catcgctggg cgacgcgtcg tccgcacggt
      541 ggatctcgag gactacccgc ggccccacgg tatcgccttc atgccggacg gtgacacggt
      601 ggtcgtgacg tccgaagcca ccgatcagat cgtgttcgtg cgcatttctg acggggccgt
      661 ggtgggatcc atcgaaaccg gcggcgccgg atcccacatg ctcgccatca ccgcgaacgg
      721 atcgcgcgtc ttcacgagca acatccagag cgacacggtc tccgagctcg acgtggcctc
      781 cgggaaacag gtgcgcgcct acccaacccc gaaacaaccc gaagccatcg cagtgaccgc
      841 tgacggctcg gaaatctggg tcggcagcaa cgctgaaggc tcgctgagcg tgatccggac
      901 cgatgacgcc accatcgaga cgattgccga aggcttcggt tggccgtacc gtatcctgat
      961 ctccagggac ggcaggctgg tgctggtccc ggacctcaag aggaacgtgc tgcgcatgtt
     1021 cgatcgcacc aatcgggtag agctagccag cgtggagttg gcggacgcgg cgccccaggg
     1081 actcgtcttc catccggaag aacgctggct tttcgtctcg ctcaatggtc agaatcaggt
     1141 ggccgtcatc gatctgagca gatccgccat agtccggcac attgacacgg gcccacggcc
     1201 tgacggcatt ggctacgccg cgacgaaggg gcagccatga gcgacgattg ctgcgccaac
     1261 ggaatcgacg cgagcggtct cgaaacgcgg caccggcgcg cgctggcgtc cgttctcgcc
     1321 ataaacgtcg ccgcattcct cgtgatgatc gtgggcgccg tgctcagcgg ctcatcctct
     1381 ctgctctccg gcaccctgga caacctcggc gacgcgttga cctacgccct tagcctggca
     1441 gtcatcggtt ccacggcatc cgcaaaggcc cgggtcgctt ttttcaaggg cttgctgatc
     1501 tttgcagccg ccctcggggt ggccgcgcag atcggctggc ggctttccaa catggagacg
     1561 ccggtgttcg aaaccatggg ggtggcggcc accctcaacc tggccgcgaa cctgatctgc
     1621 ctgcgcatcc tgtttccctt ccggaacgac gacctgaaca tgacgtccgt ctgggagtgc
     1681 tcgcgcaacg acatcttcga cggccttgca gtgctgaccg ccaccgtggc cgtgtgggtt
     1741 tccgactccg catggccgga cctcttgatc gcgatcgctc tgctgatcgt tttcctgcga
     1801 tccgctgccc gcattctcag ccgttcctgg cgggagctgc acgccgcacc cacgccgggg
     1861 ccggggaccg ggtaggaaaa cggcgaccgg atgcagcgtc aggcagatct atgctcgccg
     1921 ggccagaatc tcatccacca gctcgacgcc gccgtggtcc agggctgcct cggggaagtt
     1981 ccgaatgatc ttcctgaaga tggcctggcc gccctcggtg gagacgtcca gatcgcctaa
     2041 cgccatcggc aacagccgca ggccgatgat gatgccgtgc cggtagtcga accaggcttc
     2101 atcgaagctg taacccggga tccccgcgtc caccagccgc tggtggtaac gactaaccag
     2161 gttacgttca tgctcgcgac gaaacgccgg cgtgtacccc gaggtgatca gataccccag
     2221 atcctgggcc gttctgccgg gatacattga ctgccaatcg tagagaacga ccggatcgtc
     2281 ggattcctca tcgaggaaca cgttagccag ccgtaaatcc atgtggcaga gagacagatt
     2341 ggtttccggt tgcgcagcag cgatcgtccg catgttctcg ccgagccgaa cggccagacc
     2401 ctcgaacccg tcgggcgccc aggacatgaa ctcgctgtcg acgaagtcct tgtagccctc
     2461 ctggatcccc gagaaatagc ctacaaaggc ggggtgatcg ggccccatca gctcatcctt
     2521 tgtcgcgagc caatccgcat tccaggaaga aatcgccatg tcagcggcag catcgacgat
     2581 cagctccagc tccgccaagg tagtcggctg ctcgtcggtc tgcaccggcg cgcgcatgtg
     2641 cgagcaatcc tcgaggagca gaacaccggt tcccagctcg gcatcgtaat cgcagtgata
     2701 ggctcccggt gagcggatgg ctacgagcgg agcgatttcc gagtaaaact gcttttcgcg
     2761 cgcgaagagc ccgaactcgc ggcttagttt cctcgctccc tcgaatgtcg gcgccagctt
     2821 gcctacgagc tggcggggca acccgctccc gggccgtgtc tccagctcca gaaagcacgc
     2881 gaggctcacg aagcccgagt ccgcttcgga ggtcctcaca tcgacatcga ggacttcgct
     2941 ctcaggcacg ccaagcacct cgcacagcca gctcgtggtg aagtcctctg gagtttcagg
     3001 tatgcctgcc atctcgctgg ttctcctctc tatcccgatt tctcatccca ccgacgccac
     3061 gaccttgcca tggcgtcggg aattaggaaa gtcgccccgg cgcagccact tgccgtaaca
     3121 ttcgtgccgg accgcgatga gcagagttta aggagacaac gatgaaaaca atcaaccgaa
     3181 cgttgggtcg atccctgctg gtgctgattc ccttccttgc cctgaacggc atgttcgcct
     3241 acgccgattc ccacgggccg gctctcccat cgggcgtgct cgaagttttt cagtgcaact
     3301 acaacagcgg caaggatcgg gacgacctga acgccctgcg ggacttctac ctgaagcagg
     3361 cgaaaaaggt gaactacacg ccgcctgacg cgtttctctg gacgatgacc aatggcagct
     3421 ggcccaccac ccttacgtgg gtcaacgtcc acgaagacct gagcgcctat tccagatctt
     3481 acgataccat tgctgcctcg accgacctgg ccgcggtcaa cgagcgcgcc gagacggtaa
     3541 tgacctgcgt gcccgccctt ggaagctacg cccacgcgtt catacgcgac cccgacgccc
     3601 aaggaccctg ggaggcgacg gtggcttcct acgcctgcga tctgcgcccc gagcaggaca
     3661 tgagcgacct ggaagatcta atggaacacg tggcaacggt caatcaatcc atgggcgacg
     3721 ctgccatgga tgaactgctg cacttcagtc ccatgacggg tctaaacaac cagccggatg
     3781 tgttggtatt cgctgcgagc cggcgcggcg cttcgtcctg gtcgagccat ctcgcgctcc
     3841 tcgagcaatc gggtgccggg cgggcagttc tcgcgcactt tgattccgtg ctcgattgcc
     3901 ggttgagcct ttgggacgtg gaacagatgg tggggggata accgcgggca cgccgcccgg
     3961 ctcagcccgg ggcgaaattg aatcgcaacg acacgccgaa cgcccgcggc tcgctcacgg
     4021 aaaaatcctg ctgcgaatgc tgacggtacc cacctaggat gtgcaagtcg ttttcgatgt
     4081 tgcgcaccca ggcgcgcgcc gaccagcgtc cgtcgccggt ctcgtagatc aggctcaaat
     4141 tgtgctgatc ccaggcgtcg atggtctccg cgctgcgatt gaaaatcgtc aggtagctct
     4201 tgctctgcca gtagtagtcc caccgggcag tgagcgcccc ggcgggcacg tcccacgtgt
     4261 agctgacacc caggtgcacc gtgtgctcgg gcgtgtctgg gacggagttc cccgacaccg
     4321 gcacgggtat cccgggtaac agctccacgc ccacggcaga gagaaatgcc ccgtcgaacc
     4381 aggccgggat gccgttggga tagatggttg cgggcgcttc atccggcccg atggccgctc
     4441 ctatggcaat cgcggggttc acccagggca gcacgtcctc aacgcgggca acgtagcggc
     4501 cattgggaga tccggcgttg gtggcgaaag acagcagctc cacatattcg gggtcgccgt
     4561 tggtcagcca cagggcgtcg atgcggggtg gttcattctt gagctcggca ttgagccagg
     4621 agtagccaat ctccagctcg gctctcggcg cgaagggcgg tcgccacctg gcctcgatct
     4681 ccgcgccgta catgtccgcg tccacgttgg tgttctcgat gttgcgggta aactgctccg
     4741 cggccaggtt ggtgagctgc agcccctcat agtcgttgaa gaatgcggcc acgttcagca
     4801 ttaggctgtc gtctgccagc agcgacttga agccgagctc gaaagagttg acgtcctcgc
     4861 ggtcgtaggt cgggttggtg ctgaagaagc cgttcaggcc gccgggcttg tagccccggg
     4921 cgaaggaagc gtaggccagc atgtcgggcg tgatctgcca gtcgaggaca gcgcgtccac
     4981 tgacggcgtc ccactcgaaa tccgtcggca cgccggcaat gatcgcgccc tcgttccagg
     5041 cagtcagaat cgggatctgc gatgcgatca ggttggcggc cagaaggccg ggaaacccgc
     5101 cggtcgccag ggcggcatcc acctcctcgg tcagaccgta aaagtcgagc agacccgagt
     5161 cgtcggagat tccaaatatg taaagggccg cttggggacg cacccacacg tcgcggccga
     5221 aaacgccgcc aaggccggtg ttgatgtcga aaggggcacc gatgccgcga gctctgtagc
     5281 gtttctcgtc gcggttgtag cgcgcggcca ccgtgagttt caggtggtcg gtcagatcca
     5341 cgtagatctc cgaaaaggcc gagaagctct cgaactaatt tttgtcttcg ccgccggcta
     5401 ataccatcag acgatcgaga gcgttcagat taagcgcgaa cggattcgga cccggtgccg
     5461 gggcctgtgc cctgccaaac cgggtccaca tctcccagta gtcgttggtc ccgccacccg
     5521 agacagcgtg gtcgtccgag aactgatagt tgccgccgat cagaaagttg acgcgaccgt
     5581 ccagatccga gcgcagtcgc agctccgcgg agtagaagtc acgccactcg cgtcgttccc
     5641 cgtaccagaa ctggcgcgtc acgttggggt ccagcacgca gccgccaagc acgccaaatt
     5701 gcgcttcgtt gacgttgcac tccggcccgt cgagcgggtt cttgccttca ggcagggcgg
     5761 agaccggata caggccgctc ggattttccg gaatcggcgc caactcatgg ccgacgctcc
     5821 aatcgttatc ctgcaacgtg cgcaatcgcc agtcctgata gctgccgatg aagctcaacg
     5881 tcgcccaatc gaaatcgtgg ctggccccga gctgccagag gttctgctcg aactcgtaca
     5941 cgggggcgaa gtcgatgtgc acgtccctga gattggtgat ctcggggcgc gggtaggcaa
     6001 acgtgaggcc ggtttcagcg tcgcgcgccc caaggggaat gacgcctcgc aggccgttgg
     6061 tgatcgaggt ctgaaagcgg gcctggttcg ggttgttcgg ctccaggccc cactcgccgg
     6121 gctcgcagcc agtgcgcgtc gtgggccgca gcgccgtttt gcaacgacgg ttgtgggtca
     6181 gcatgcggtc gtcgtcttca tcgaagcgct cgtacaggcc ccagatctcc gtcgcctccg
     6241 tgacgcgcca ggtggcgctc gcccggacgc tgtagagatc tcgtccgtcg atctcgctcg
     6301 ccaccccggg cacctggccg ccggccaggt tgtccgtgta gccatcccgg tccagagaga
     6361 gtcccgctag gcgaagcgcg agccggtcgg cgaccgtggc gttcagcgcg ccgcgcagcc
     6421 gcaggaggtc gtggttaccg gcctccacgt ccaggtaccc gcttgtaccg tcaaagctcg
     6481 gcctgttggt aatgaggttg atgacaccgc ccgtggcgtt gcggccgtac agcgtgccct
     6541 gggtccccgc agcacttcaa tccgggcgag atcgtagatc tcgaacggcg gctggccgga
     6601 tggcaagggc acctggttga cgtgcaggga cacgcccgac tcgctgtccc ggcgggacac
     6661 caacgacccg acaccgcgaa tggtgacgtg ggtgtccccc acgttgttgg tgttgtagct
     6721 caggttggga acgaacaggg aaagatcgcc gatgccaatg atctggcgat cctcgatgcc
     6781 catctcgtca aatgcgttga gggcgatggg caccttctgg gcgctctcct ccacccgttg
     6841 agccgtgacg atcacctctt cgatgaccaa gcgctcctga gcctgcaggg cgcacggcat
     6901 catcaggacc atcgcgctga tcgggattcc tcgggccatg ccgcgtctcc ctcttctgcc
     6961 gagctaactt ataacgccac gagagccgcc tgaccaccca tggccgtcta ggccgttcgc
     7021 cgaccgcggg gatcgacgat ggcatcggac attcgagccg ctcgcgccgg gcgtgcgcca
     7081 cgaaaatcgg cctcgcggag cgctaacgca gaaactcgat gtagaattcg acctcgccga
     7141 cgggagcgac ctcgtggagc accgtcggct cgacaacgcc catgatgtgt ggggagagca
     7201 gaaactctgc cgggtctggc tcgaggatcc gataaaggag ctcacccgtc acgacgacga
     7261 tccttcccca ggtgccgggc ttggtgctgt gcgccttcag caatccgggc ggaatcgagt
     7321 cctggtgaaa agtcgccgta cgcctgtagg gaagtacgtt ctctggcaat gctcgcatgt
     7381 ggagagtaaa cgccatccgt ttaatcgccg ccacctcgcc gcaccaatcg aggatgcccg
     7441 gtgtcggaga tcccgacgag ttcctgcgcc aggcgtaacc gcctagaagt taaccccgcg
     7501 ggtaaatgcg ccgtcgacgc gcacggtggt gccgttcacc caactcgcgg cggggctggc
     7561 gagaaaggcg acaacgcgtg cgacctcttc ccccgtgccc atgcgtttgg tgggatggta
     7621 gtcacggtcg cgctcataca gggctggcgc gttgtccttg atgccgttcc aaacgccgtt
     7681 ctcgatgaag atcggccccg gcgcgacggt gttcgcgcga atgcctttgg gtccccactg
     7741 ctgggccaat tgcgccatgt gacgaatcga cgcggctttc atggcaccgt aactcgggct
     7801 gatggggacg tcgtggtgct caatgccggt gatcgacgcg atctgtacca gcgctgcacg
     7861 ctcggattgc tcgagaaagg gcagcgcgcc ttcgctcatg cccaccatgc cgagcacatc
     7921 gacgctgaag ttgttgatcc aggattgggg tcccggaccc ggttggccgc tggccgtggc
     7981 gttgtgcaca acgatgtcga taccgccgag ggcgtccgct gcgttctgca tccaagccac
     8041 cgtggcgtcg tagtctgcaa cgtcgcacac ctggccaacg acggtgccca gcgcggacag
     8101 ctcctcgagt gccgcgtcca cgccttcttg ccggcgcgcc gcgatgctca ccgccacccc
     8161 ctcgacgatg agctgtttgg caaccgccag gccgatacct tgcgtgccac cggagatgac
     8221 ggcttttgcc ccattaagtc cgagatccat gtgctccctc ccaggttctg tatcgcctta
     8281 tagtcgcaga accggcatca cggtaacaac aggcgccccg cctgcacggt gggcggcatc
     8341 gccggaatcc ggacgcgccc gttggctccg gcatgtttaa gaggatcgct tagtgaccac
     8401 tgggtccccg ccggttgcct ccatggtggc tttacccatc ttgcggccgt cgaaaccgat
     8461 ggccttggtc ggcgtgaact tgatgacacg gcggttttcg gtgttgttca gctcaacaaa
     8521 gacgcgccgt gctcgctcgt ctcctatccg taaggctttt gccagctccg gcaggaacca
     8581 atcgacggtg gcttggtctg agaacacttc gcaggtgccc ttgtaggtga ggctctgtcc
     8641 gctgcgcagc ggtgtgccct tgcttgttat cgagatcgac gcgcggggat cgcgctgcac
     8701 cgccttcacg cgaacgcgta aagacgacac gctgagccag aagcaatcct ccgcgtagac
     8761 atacgtcatg atcaccccga ccggccagcc gtctttggtg gaccacatga acgtgcactc
     8821 gttctgcgcg gcgatgaggg caagctcgtc ggccttgtcc agggtgtagg tgctgacgtc
     8881 gtcgtagttg aattctttgg acatcttact cgggtcttcc agcagcaatc gcgtgtgaga
     8941 gcgtatcatg cgccgtcgtt ccgatccggt caccgctcaa ctccactttt agggggagcg
     9001 atgtcagcgc agaggcggat cccgggtggg ttcgcctgga cccctccccg tcggcgaact
     9061 actatgaggc aaccaatcaa taagggaaaa acggatcatg gcagccgtca tcgaagaaaa
     9121 accctcccac ctgcgcggca acggccgacc gatcaccgag gagcgcacgc tcacggatct
     9181 gaaggtgacc ggagcaattc ctcccgagct ggacgggcgc tacgttcgca acgggccgaa
     9241 tccgctcagc ggtaccagcg cgcatccatt cttcggcgac ggcatgctgc acggcgtgca
     9301 tctgcgcgac ggaaaggctc agtggtatca caaccgctac gtccagacgc ccttcatctc
     9361 aaacccttcc gtggacattc tcgatgcgtc cgtcatgctg gacatgcggg cgtcgaaggc
     9421 gaacacccac gtcgtgggcc atgccggcag gatcctcgcc ctcgaggaag gtcatttccc
     9481 ctatgaggtg gacgcggatc tgaatacggt gggacccgtc gatttcgacg gcgtgctcac
     9541 cggatcgttt accgcacacc cgaagatctg cccggtcacc ggcgagatgc tggcgttcgg
     9601 ctactcggcc ttcgagccgt acctgcgcta tctgcgcgtg tccgctgaag gcaagctggt
     9661 tcagacagag aacatcaccg tgggcggccc gacgatgatg cacgacttca acatcacgaa
     9721 gaatttcgtc atcttcatgg atctgcccgc cgtcttcgac atgagcctag ccatgagcgg
     9781 cgacatgccg atccggtggg acgacgacta cccggcgcgg ctgggcgtta tgccgcgcac
     9841 cggcaccgat gcccaggtga cgtggtacga catcaatccg tgctacgtct tccatcccat
     9901 gaattcctac gaggacggcg acaccatcgt cctggacgtt gcccggttcg atcacatctg
     9961 gcgcgaatcg gcaatggact tcgcgccgcc gcagctctgg cgctggacga tcgacactgc
    10021 gaccgggcgg gttcgcgagg agcagatcga cgaccgaccg gcagagtttc cccgcgttgc
    10081 ggacagcctg atcggcctgc gacaccgata cgggtacatg atggcgatgg ccaccgggga
    10141 ccagggggat ccggcgagcc agtcgggcgc gatcctgaag tacgaccggg agaccggcag
    10201 ccgctcggag atcgagctcg gtcgcggcca cacctgcggg gagcccgttt tcgcgccggc
    10261 agccgatgcg aaggccgagg acgacgggta tctgatgacc tatgtctacg atgccgaaac
    10321 ggacgccagc cgattcgtca tcatggacgc ggccacaatg gacgacaccc cggttgcgtc
    10381 gatcgagctg ccacgaattc ccaacggttt tcacggcagc tggattccaa gctccgtggc
    10441 cggctgaggg ttcatcggca ccggaaacca ccacaggcgc cggatacgac gcaccggcgc
    10501 ttccacccgc gcttgcagcc gcgcttgcag ccgcgctcag agcatgaatc cgccgtcgat
    10561 gacgagctcg gaaccggtga cgaaccgcgc gtcgtcgctg gccaggtaaa gaatcccgtt
    10621 ggcaacgtcg cggggttcac cgaggaagcc gatggggtgt tgctggacga gccccgccat
    10681 tatgtccgcg ctgtttcccg agcgctccat ccactctttc atcatcgtgg tcaggatgta
    10741 gcccgggtgc accgagttcg cccggatgcg atagccgcac tgggcacagt agagggcgat
    10801 cgatttggtc agcagcctga ccgccccctt gcccgccgag taggccgcct cgtgcgcggc
    10861 cgcccttatt ccgtgaaccg acgacacgtt gacgatggcg ccgccatccc gcttcatcag
    10921 ctcgatggcg agcttgctgc cgatgaacac actgtcgacg cacacccgca tcaccgcacg
    10981 gtagtcgtcg agagtctgat gctcgatgtc gccgatgccc gcaccgatac ccgcattgtt
    11041 gacgagaata tcgagccgac cgtaccgatc ggtcacttca ttaatcacgc gcttccagtc
    11101 ggcttccgag ccggcatcat gttccaggcc catgccgccg aattcgtcag cgagcttcac
    11161 ggctccctca ccattgatgt ccgtgacgac caccacggca ccttcctcag cgagcagggt
    11221 ggccgttgat gcgccgatgc catcggctgc agcggtaacg agcgcgatcc gatcctttac
    11281 cttgttcatg gctggttgac ctccccccgg aaccagaatg agcggactga catgaaggct
    11341 gattatactg ccgccggcgc gggatagccg gttgccgaag gacccgccgc ccaagaggtg
    11401 aaggagtagc ggcaccatga accaagaaga ccagatgcga ccggacgcgg aaagcaggta
    11461 ccatgctctt cctggcggca tcaatggcaa ggagcatgcg gtgaaccttc tcgagaaagg
    11521 cagtttacgg tggaagcgct tcaccggagg cgacaagttc gactatccca tcgactattc
    11581 ggcagcgctg ctcaacgcgc gggaagacgg ccacgtggac ctgctctacc gctgggagcc
    11641 caactgctac tgccactttc accgccatac ggcggagacg acgtccaccg tcctcgaagg
    11701 cgaacttcac gtcatcgaca tcgatctcga gaccggcaag gaagtgggcc gcagggtgag
    11761 gcgtgcggga gactacgccc acaaagcccc cggggacgtg cacatggagc gcgccgggcc
    11821 ggagggtgcc ctggtgctct tcagcctgta tgccccggat ggcctgctgg cggaatccct
    11881 cgccgaagat ggcacggtgc ttggcgtctc gaccatggat cgcatcctca agaggcgagc
    11941 atcgtaatcg caccagttga cggcgcgtca gcgcgccggc cgcccctgag cccaaatccc
    12001 ggaggaagga aaggtcaatg cgtgtctaca aagatgccgt gatggacaac gcccgctggg
    12061 aaggcttcga gccccgtccc ggcgacatct tcgtgtgcac gccctccaag tgcggcacga
    12121 cgtggatgca gatgatcgtc gccaacctgc tctggccgga cggcgagttt ccgggctcga
    12181 tcgtcaacgg catatgcccc tggatcgagg ccaaattcac gccggcggaa aagatgcacg
    12241 ccatgctgcg tgcccagact caccgccgtg ccatgaagag ccatacccct gccgacggca
    12301 taccctggtt cgacgacgcc cggtacatca ccgtggttcg cgacggccgg gacgcattca
    12361 tgtcctggtg caaccacgtc agccgcatga tggtcaccga catgctcaac gagaaggctt
    12421 ccgaggacgg cctgccagcg atcaacacct tcgacggcgt cgactaccgc ggctacttcc
    12481 acgagtggct ggagcggaac aacttcttcg acgtggtcgc aagctattgg gccgtccggg
    12541 accggccaaa ccttctcttc gtgcacttca acgatctgaa ggcagacctg acggggcagg
    12601 tgcggcgcat cgcgccattt ctcgacatcg agctcgacga ggaccaatgg cccgcggtgg
    12661 tgaagcgctg cacgttcgag ggcatgcgca aagcggacaa gaaggtcggg gacttcgagg
    12721 ggttcttcga aggcgggctc gaaggattcg tctacaaagg cacgaacgga cgctggcgcg
    12781 atgtgctgaa cgacgacgac attgccgcct accgccgcaa ggtcgccgaa accctcccac
    12841 cggacgcggc tcgatgggtc gagttcggcg cccgagccac ctgatcccgc cgagtactcg
    12901 atcgcttcgc ccggctgaca atcgcgtcat attcgccagc gaactgaccg ggtatcgtga
    12961 gatgggcaat cgattgagcg caacgagtat gagcatccgg gcacgcggcc gtcttctcat
    13021 cgcagcgttg agtgggttgc tccttcccgc cgcgtacgcg gtcaccatcg acatagcgga
    13081 ggtccgggac gaaatggcct tcgtcaaagg cagcgatgcg ggccgaaagc agcaaatact
    13141 gtgggaaggc gcacccgcca caaccgcgaa cggcaagggg aatttcagct ttgagggccc
    13201 cctgcccaat ccctgcgtac cggaagattg cagcgggacg ctgactgccg gcgaccatgt
    13261 agagccggta acactgacct ttcccccggc ggagccgcct gtccagacgg acgaggcgct
    13321 cgttcaggtc gccgcccaag ccgacgcgca caacggcgaa gcgctccgga cggtgggctt
    13381 cgtggacgac ggtttaacca tcaaggtggt ctccgggggc gccgatacca acctgcgcta
    13441 ctggaccaat gagttgcatg aactggaaat cggccacgat ctgcccaaca tcatctacga
    13501 cttggaagtc tctaacgact actccgctgt cgtaacaggc gaaggcgggt ggaacggaag
    13561 cgcgggcgcg tcaacgacgt cgccttttct ccggaaggcg acctgatcgt ctccggtggc
    13621 gccgacggga cgctcaagct gtgggcttat cccgaccctc cccctccgga ttagggttct
    13681 tttcttccct gatccgctat tcttgcctgg cagtccacct ggaacgcgtc gggcgacgca
    13741 cactgcgcaa tggccgatgg ttttcgtctg aacggagtga ccggctaagg aaatgacagc
    13801 acaaagatgg tcgaccgaac gcgccgcgcg ttggcaaacc gacgtgggct ggctcgtggg
    13861 ctgcaacttc acgccctcga cggcgggcaa ccagctggag atgtggcaga gcgaatcctt
    13921 cgatcccgag accatcgacc gggaacttgg atgggcgtc
//
LOCUS       DBNHOX010000194         2825 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1622928, whole genome shotgun sequence.
ACCESSION   DBNHOX010000194 DBNHOX010000000
VERSION     DBNHOX010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1622928"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1676)
                     /locus_tag="AC16UZ_04275"
     CDS             complement(<1..1676)
                     /locus_tag="AC16UZ_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6245931.1"
                     /translation="MSTLHKPTCLIALFLSVTLMTPPGWTAAREIGPNHWAGVSRVVA
                     FGDVHGAHDQMVTALESAGVVDAELNWAAGDTHLVSLGDLLDRGAHSRQTMDLLMRLQ
                     RQAKGAGGRVHVVLGNHELMNLTGDMRYVAADEFAAFADDEDPSVREAAREAFVAAAD
                     PASGKDPSVEFDERYPAGYFGHRAGFAAAGSYGDWLLQLPLMVVVNETAFVHGGLPAL
                     IADQTLEEINRQYQRDLGEIMALGGRLADAGWVSAERDLLLQDDAVLARLEAAAEGDD
                     GDLSRAGTRFIELVRGPLFGSRGPNWYRGTAMCHASLEETVLKAALKALGAKRVVVGH
                     TPTSNRRVQQRMDGRAILADTGMLRSYYSGQPAPVVLTGDDIEILYPLSETRADAPDV
                     IDPLARYGLDQPRLEAVMSNAAIAEVSTAGTGQRFTTVTLLDEGKRYEFAFERGQGSD
                     NDRKVGAYRLSRLLELNVVPVTVSRTVEGAKGVLIALPEKRVSEIERANQGLSRSNWC
                     SFGNDYQLMYAFDALIRNEQRTTDTMIYDQEDWQLTLIGHEASFGNGTDLP"
     gene            1795..2193
                     /locus_tag="AC16UZ_04280"
     CDS             1795..2193
                     /locus_tag="AC16UZ_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6245932.1"
                     /translation="MHPNEALFANESFYLAFAAGDSDAMEQLWSRQHPLVCIHPGWPA
                     LVGRESVIDSWRQILANPDQPEVEFYGARAVELGSGIAVVCYEVLPGTVLVAMNLFIA
                     ENGEARLVHHQSGPCGDPPPRDAVVRRLDA"
     gene            complement(2197..>2825)
                     /locus_tag="AC16UZ_04285"
     CDS             complement(2197..>2825)
                     /locus_tag="AC16UZ_04285"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6245933.1"
                     /translation="LPCKPVWGAPDHEALNYNLPDFDPLWACVQDAGLPVTFHVSTGR
                     DPRTARGNGGAVINYAVHSLAPTMEPIANICASGVIDRFPKLRFGTIEAGIGWVPWTL
                     AALDEAYKKHHMWVRPKLKMLPSDYFRQNGFASFQEDQPGLDLAREHRLIDNFLWAND
                     YPHHEGTWPHSAEAIERTMGHLTDSERAKILGLNAASIFGFEVPRGKA"
BASE COUNT          477 a          922 c          903 g          523 t
ORIGIN      
        1 ggcaggtcgg tgccgttgcc aaagctcgcc tcgtggccga tgagcgtcag ctgccagtct
       61 tcctggtcgt agatcatcgt atcggtagta cgctgctcgt tgcgaatcag cgcgtcgaaa
      121 gcgtacatca gctggtagtc attgccgaag ctgcaccagt tcgaacggct cagtccctgg
      181 tttgcccgct cgatctctga aacgcgcttc tccggcaggg cgatgagcac gcccttggcg
      241 ccctcgacgg tgcggctgac cgttacgggc acgacgttga gctcgagcag gcgggacagg
      301 cggtaagcgc cgaccttgcg atcgttgtct gaaccctggc cgcgttcgaa cgcgaactcg
      361 taccgtttgc cttcgtcgag cagcgtgacg gtggtaaacc gctggcccgt cccggcggtg
      421 ctgacttctg cgatggctgc attgctcatg acggcctcca gccgcggctg atccagcccg
      481 tagcgtgcca gcgggtcgat gacgtcagga gcatcggcgc gggtttcact cagagggtag
      541 agaatctcga tatcgtcgcc ggtcagcacc accggggcgg gttggccact gtagtaagag
      601 cggagcatgc cggtatcggc aaggatcgcg cgaccgtcca ttcgctgctg aacgcggcgg
      661 ttcgacgtgg gggtatgacc cacgaccacg cgctttgctc ccagggcttt cagcgcagcc
      721 ttcaacaccg tctcttcgag ggaggcgtgg cacatggcgg tgcctcgata ccagttcggt
      781 cctcgggatc cgaagagggg gcctcttacg agctcaatga aacgcgtgcc cgctcgggaa
      841 agatcgccgt cgtctccctc ggcggcggct tccagccgcg ccagcaccgc atcgtcctgc
      901 agcagcagat cgcgctcggc gctgacccat cctgcatcgg ccagccggcc gcccaacgcc
      961 atgatctcgc caaggtccct ttggtactgc cggttgatct cttcgagcgt ctgatcggcg
     1021 atcagcgcgg ggagcccgcc gtggacgaat gcggtctcgt tgacgaccac catcagcggc
     1081 agctgcagca gccagtctcc gtagctgccg gccgcggcaa atcccgcccg gtgaccaaag
     1141 tatccggccg ggtaccgctc gtcgaactcc acagatgggt ccttgccgga agcggggtcc
     1201 gccgcggcga caaacgcctc gcgcgccgcc tcgcgcaccg acggatcttc atcgtctgca
     1261 aacgccgcaa actcatcggc ggccacgtag cgcatgtcgc cggtcagatt catgagctcg
     1321 tgattgccca ggacgacgtg cacccggccg cctgctccct tggcctgcct ctgcaatctc
     1381 atcagcagat ccatggtctg ccgggaatgc gcgccccgat cgagcagatc gccgaggctc
     1441 accagatgcg tgtcgccggc ggcccagttg agctcggcgt cgaccacgcc agcggattcc
     1501 aatgcggtga ccatctggtc gtgcgcgccg tggacgtctc cgaacgcaac gacgcgagac
     1561 acgccggccc agtgattggg accgatttcc ctggccgccg tccagcccgg tggcgtcatg
     1621 agcgtcacgc tcagaaaaag agcgatcagg caagtgggtt tgtgcagcgt gctcaaagca
     1681 gaagtccgac ccgagtcgcc cccatttcac gaaaagccgg ctccatttgc aacgacggac
     1741 gccgggcgaa ctcgccttga gccattctcc cagagcgtgg atactcgcgc catcatgcat
     1801 ccgaacgaag cactgtttgc caacgagtcg ttctatctcg ctttcgcggc cggcgactcg
     1861 gatgccatgg agcagctctg gtcacggcag cacccgcttg tctgcattca tcccgggtgg
     1921 ccggcgcttg taggccgtga gtcggtcatc gacagctggc gacagatcct cgctaatccc
     1981 gatcagcccg aggtggagtt ctacggcgcc cgggcggtgg agctcggcag tggcattgcg
     2041 gtggtctgtt acgaggttct gccagggacg gtgctggtcg ccatgaacct gttcatcgct
     2101 gagaacggcg aagcgcgcct agtccaccat cagtcggggc cctgcggaga cccgccgccg
     2161 cgcgacgcgg tcgttcgccg cctggacgcc tgactcctag gccttccccc gcgggacctc
     2221 gaagccgaag atacttgccg cgttgagacc caagatcttt gctctctcgg aatccgtcag
     2281 gtggcccatg gttcgctcga tggcctcggc cgaatgaggc catgtgccct cgtggtgggg
     2341 atagtcgttt gcccacagaa aattgtcgat cagccgatgc tcgcgggcga ggtccagccc
     2401 gggctgatcc tcctggaagc tggcgaagcc gttctgccgg aagtagtcgc tgggtagcat
     2461 cttgagcttc ggcctcaccc acatgtggtg ttttttgtag gcttcgtcca gcgccgcgag
     2521 ggtccagggc acccacccga tgcccgcctc tatggttccg aaccggagct tcgggaaacg
     2581 atcgatcacg ccggacgcgc agatgttggc gatgggctcc atggtcgggg ccagcgaatg
     2641 cacggcgtag ttgatgacgg cgccgccatt gccgcgagcc gtgcgcggat cgcgcccggt
     2701 ggaaacgtgg aaggtgaccg gcagacccgc atcctgcacg catgcccaca gcggatcgaa
     2761 atccggcagg ttgtagttga gcgcctcgtg atccggagcg ccccacaccg gtttgcaggg
     2821 aaggg
//
LOCUS       DBNHOX010000195        12415 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1365646, whole genome shotgun sequence.
ACCESSION   DBNHOX010000195 DBNHOX010000000
VERSION     DBNHOX010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12415)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12415)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12415
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1365646"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="AC16UZ_04290"
     CDS             <1..1452
                     /locus_tag="AC16UZ_04290"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR6245934.1"
                     /translation="LDVEDGSAQRIGHRGSGLPEPVVVPHGWPRRAFLSAAMTAAAAG
                     CAQFGVPPLKRLYQSVPVPEDQPPLIVIPGAFGSKLRRRATGEEIWPRSGFALLFSSY
                     PDVEVTIDADTLDPIADEVEAFDIFRKGMGRDFYGALLKTLESIGGYQRAHPGTPPAP
                     PARSYYVYPYDWRLDNVAAVEGLHRLIEQVVEDHGDPNLRVDILGHSNGGLMARYYAR
                     FGTADMLTSRAAEPDWNGSHRIRRLLMVGTPNLGTMQPVLSHVRGEEIGLNDIPAEVV
                     ATCSGAPQLFPAPELTWLVDARGEDLNLDVYALETWRELGWSIFSDKARDRATSKHGV
                     GRRGRAHLELLERYLAKHLERGRRFQHALARPARPDEPEPFVFGGDCTPTVARLVLEK
                     RGGVFVGREQPDRVAAPVRGVDYDGLINEPGDGVVTRASLTGQNATPVAPQLNVAHSV
                     FLCEEHQLLTSNASFQDNLLYNLLTEFPQGAHG"
     gene            1498..2196
                     /locus_tag="AC16UZ_04295"
     CDS             1498..2196
                     /locus_tag="AC16UZ_04295"
                     /inference="COORDINATES: protein motif:HMM:NF016764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0016192 - vesicle-mediated transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HIR6245935.1"
                     /translation="MSGVHEPDTAVYTRRREELIDEGSGSLAVTTSIPASMMLRLLIR
                     PRSVFRLLADADPAPLVVFFKFALWLGLLPPLFAYIGASTFGWRVGVVEPLFLPQPTL
                     IAISIAYFCSLLFGFFSASIISRWMAVTYGARDSLGVHLAMMVIVGAPLAIASLMHLY
                     PNALLNAVVFVPTMIWSMYLLYRGLPTILHTTPEQGMLMASSIIGYLLVAAVSLLGLT
                     VFLWVMGIGPALGV"
     gene            2200..3639
                     /gene="ccoN"
                     /locus_tag="AC16UZ_04300"
     CDS             2200..3639
                     /gene="ccoN"
                     /locus_tag="AC16UZ_04300"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045277 - respiratory chain complex IV
                     [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HIR6245936.1"
                     /translation="MSTTNDQTYSDRVVTTLVLLAIAWSIVGMTAGVWAAAQLVWPTL
                     DFGQSWLSYGKVRTLHTNAVIFGFGVTALMGTAFYSVQRTSHVRLFAPGLAWFTCWAW
                     QITVLIGGLSLLAGYNAGKEYAELEWPLDIAITVAWVCFAVVFFGTIARRRIKPIYVS
                     NWFYGGLIIVIAMLHIVNSLALPVTLTKSYSLYAGAQDAIVQWWYGHNAVGFLLTGGF
                     LGMMYYFLPKQAEQPIWSYRLSIVAFWAFTYSYIWAGPHHLHYNSIPEWVQSLGMVMS
                     LVLLAPSWATMVNGIMTVSGAYEKLRTDPAMKFIVLSLAFYGLATFEGPMMSIKSINV
                     VSHFTEWTIGHVHSGALGWNALITFGTFYFLVPRLMGSKLYSVKLANWHFWLALAGTM
                     LYIVAMWGAGVSQGLLWLNIDDMGELRYSFTDIMAATVPYYVIRLLAGAVFLVGALLM
                     AYNLYRTVSGKRTIEVHAPSADDAAGASA"
     gene            3636..4259
                     /gene="ccoO"
                     /locus_tag="AC16UZ_04305"
     CDS             3636..4259
                     /gene="ccoO"
                     /locus_tag="AC16UZ_04305"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020800672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HIR6245937.1"
                     /translation="MSGIGLHRKLEENSGLLIFGILFVSAIGGLVQVLPSLFQESLKT
                     PAANTRVYTPMELVGRDIYVREACSTCHSQQVRPLFAEVRRYGPYSRSGEFVYDRPFL
                     WGSKRTGPDLHRVGGKYSDAWHEIHMIDPRAVVPDSIMPAYPWLAERAANADGDIEAK
                     LRGLVTLGHPYTDEDIAGAAASLEGATELDALIAYLQMLGTGMEETP"
     gene            4256..4399
                     /locus_tag="AC16UZ_04310"
     CDS             4256..4399
                     /locus_tag="AC16UZ_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245938.1"
                     /translation="MSYFLFAGDMLVMAFMTILVVWVSLKSSGDQLERAARIPLEDER
                     HDG"
     gene            4392..5309
                     /gene="ccoP"
                     /locus_tag="AC16UZ_04315"
     CDS             4392..5309
                     /gene="ccoP"
                     /locus_tag="AC16UZ_04315"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HIR6245939.1"
                     /translation="MADMPSDFWSGWIITLTVASLAGLVWLIFSVYFGKDPDSDHTSP
                     VWDETLSEGANPAPMWWFWMILAALVFSAIYLMLYPGLGSHAGIMAWSQGGALEKSMN
                     RFDAAFAERRQGVVSAELADLHDDVDAMGTARGIFTRNCAACHGPQGLGQANAFPNLR
                     DTAWQWGGTPEAISQSITAGRRGAMVGWLPVLGEDGVEQLVTYVKTLSAGTSDPNAPA
                     ATQFTTYCSACHAADGTGVAMLGGPDLTDDVWLYGGSDDALRTSIAEGRNGIMPAFGD
                     RLDDVQLKLLVAWLTRPPKASARTEGAAR"
     gene            5346..6473
                     /locus_tag="AC16UZ_04320"
     CDS             5346..6473
                     /locus_tag="AC16UZ_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR6245940.1"
                     /translation="MNETDRRAQIGLVAVIGLILWFGLNPFDFARINNVEWLDPGPGL
                     QFGDSGLAVSEQAFTWPSDTGPAAVTVEMWVRASRVSDTEPRAWLVLMDDSKLTPLGV
                     RQEGGDLVVWDAVTNPDGERWYNDFRVPYAMRPGIEQHIAITSHEDGPLIYIDGSSAG
                     TYAGYPIPLARKGEPFGGRLVVGAGPPWVTPWMGEVLGLALYDRALTATEVEQHANAG
                     PGSGFAELARNSGAVGAYQFDAGGGTSVQDQAGGGHDLKIPEYFRPPGRGLLRSVDPA
                     ERTALWNVGDAILNVLGFAPLGFLISIVLRKHVKPTSLILTATMVGFVVSLGIEWTQS
                     LMITRSSSLQDLVLNTVGSLVGALAALASSRFRGRENNTKA"
     gene            complement(6516..6592)
                     /locus_tag="AC16UZ_04325"
     tRNA            complement(6516..6592)
                     /locus_tag="AC16UZ_04325"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6556..6558),aa:Met,seq:cat)
     gene            6677..7450
                     /locus_tag="AC16UZ_04330"
     CDS             6677..7450
                     /locus_tag="AC16UZ_04330"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6245941.1"
                     /translation="MDKPRTALITGASAGIGKAFADALAAGGVNLVLVARRADRLQQA
                     AQALREAHGIDVATISADLSDPTAPVRIHEETESRGLVIDYLINNAGVAGPDLLEPDT
                     WQANAGFHQLMMIAPAHLCHLYVPAMRARRWGRIVNVASVAARIPRAGGCNYGPAKAF
                     LVALSEELSLTLKAEGIKVCALCPGFTHTEFHNEGSAAELKANTPAFVWYEAETVVKE
                     GLRALERGRSVYVSGRMYRWMDPFFQSVWTRRLFKIAAS"
     gene            complement(7498..8589)
                     /gene="ychF"
                     /locus_tag="AC16UZ_04335"
     CDS             complement(7498..8589)
                     /gene="ychF"
                     /locus_tag="AC16UZ_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR6245942.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIDAENFPFCTIDPNTGVV
                     PVPDPRLERVAQLARSAKIVPTSMEFVDIAGLVAGASRGEGLGNQFLAHIRETDAIAH
                     VVRCFEDDNVTHVSGAVSPADDIEIINTELALADLETAERVFDRTRRIANAGDKDAKL
                     LVAVLDKVIGGLNTGRPVRALALGPEELKLLHECHFITAKPVLYVANVAEDGFDNNPL
                     LDGVRAIAAAESSEVVVICNKIEAEIVELEEDERAEFLEDLGIEEPGLNRVIRAGYRL
                     LGLHHYFTAGPKEARAWTVRIGTRAPQAAGVIHTDFEKGFIRAEVIAYDDYVGCGGEQ
                     GAKDTGKWRLEGKDYVVQDGDVILFRFNV"
     gene            complement(8590..9198)
                     /gene="pth"
                     /locus_tag="AC16UZ_04340"
     CDS             complement(8590..9198)
                     /gene="pth"
                     /locus_tag="AC16UZ_04340"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR6245943.1"
                     /translation="MADVSLIVGLGNPGPRYELTRHNAGAGFVEYLANRFGVVLRDEA
                     RFRGRMGRGEVLGHDVRLLIPTTYMNLSGSSVAAVARFYKLAVDEILVAHDEMAFEPG
                     MLRLRTGGGDNGHNGLRSIISQLGNAAGFPRLRIGVGHPGDRGRVEAYLTTERMPESE
                     RGQMEAAWRMEDAVLAAILDGDLQAAMNALHAPAGAGDEGED"
     gene            complement(9201..9890)
                     /locus_tag="AC16UZ_04345"
     CDS             complement(9201..9890)
                     /locus_tag="AC16UZ_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HIR6245944.1"
                     /translation="MTQRIEIHAELREDVGKGASRRLRRLADKVPGVIYGGDADPVPL
                     MLSSNELSKAMQLEAFYSQILNVVLDGKGEQAVVRDLQRHPASEKVQHIDFLRIRADR
                     LIQVSVPLHFIDEDKCIGVKQGGGQIVHNMNEVEISCLPGDLPEFIEVYMAQVDVNQS
                     VHLTDLALPEGVSIVALMHGSDRDANVVSVQMPRGGLEEEEAAEAAEAAAEEGGEASD
                     EGAGGEEASED"
     gene            complement(9960..10904)
                     /locus_tag="AC16UZ_04350"
     CDS             complement(9960..10904)
                     /locus_tag="AC16UZ_04350"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR6245945.1"
                     /translation="MSNLMIFTGSSNPVLSARIAEELRMDLGHADLGRFSDGEVNVEI
                     HENVRGQDVFVIQSTCAPTNDNLMELMIMSDAIRRASAQRVTAVIPYFGYARQDRRAR
                     SARVPISAKVVADMLGSVGIDRMLTVDLHADQIQGFFNMPVDNVYGSPVLIDHMLGEH
                     YENPIIVSPDVGGVVRARAVAKQCNDLDLAIIDKRRPQANDSRVMNVIGDVRGKTCIL
                     VDDIVDTAGTLCTAAEALKEEGAIGVVSYITHAVLSGPAIERIAGSELDRMVVTDTIP
                     LSGSAESCSKIEQLSLARLLAEAIRRVSNEESISAMFR"
     gene            complement(10971..11045)
                     /locus_tag="AC16UZ_04355"
     tRNA            complement(10971..11045)
                     /locus_tag="AC16UZ_04355"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11011..11013),aa:Gln,seq:ttg)
     gene            complement(11195..11725)
                     /gene="lolB"
                     /locus_tag="AC16UZ_04360"
     CDS             complement(11195..11725)
                     /gene="lolB"
                     /locus_tag="AC16UZ_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HIR6245946.1"
                     /translation="MALCAGCASFRPPVETPADFEVRGKAGVVEGAESFSARFVWRQY
                     GSAFTIDLWGPLGQGRVRLDGDERRLAIVDGKGAVISEGPHAEVMRTHLGWSLPLDVL
                     PSWVLGKPGEGTGAGGRQYDDDGRLVAFEQLDWSVAYARHNQVQTARGSRWLPGRITA
                     VRGDYRVRLSISDWVL"
     gene            11834..>12415
                     /locus_tag="AC16UZ_04365"
     CDS             11834..>12415
                     /locus_tag="AC16UZ_04365"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:NF017051.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR6245947.1"
                     /translation="MAILAYGINYRTAPIDVRERVAFPEDALPAALEDAMSSIPDVSE
                     IAIISTCNRTELIVSAAPEAEESVARWLASYRPVSMGELNSMAYSHWDQDAARHLIRV
                     AAGLDSQVLGEPQIMGQVKQAYELARAAGALGSELSLLSRLTLNAAKRIRTDTDIGRN
                     PVSVAYAAVTMAQQIFADLHHNKALLIGAGDTIQ"
BASE COUNT         2146 a         4092 c         3872 g         2305 t
ORIGIN      
        1 ctggatgtcg aagatggttc agcgcaacgc atcggccatc gaggatctgg attgcctgag
       61 cctgttgtag ttccacatgg ttggccgcgc cgcgcctttc tgtcggcggc gatgaccgcc
      121 gcggccgccg gctgcgcgca gttcggcgtg cctccgctga agcggctcta tcaatcggtg
      181 ccggtgcccg aggatcagcc gccgctcatc gtgattcccg gtgccttcgg ctccaagctg
      241 cgccggcggg ccaccggcga ggagatctgg ccgcgctcgg gcttcgcgct gctgttcagc
      301 agctaccccg acgtggaggt caccatcgat gcggacaccc tggatccgat cgccgacgag
      361 gtagaggcct tcgacatctt ccgcaagggg atgggcaggg atttctacgg cgcgctgctg
      421 aaaacgttgg aatcgatcgg cggttaccag cgggcgcatc cgggtacgcc accggcgccg
      481 cccgcgcggt cttactacgt gtacccctac gactggcggc tggacaacgt tgctgctgta
      541 gagggactcc accggctgat cgagcaggtg gtcgaggacc acggcgaccc gaatcttcgc
      601 gtcgacatcc tcggccactc gaacggcggt ctgatggctc gctactacgc tcgcttcggc
      661 acggcagaca tgctcaccag tcgcgcggcc gagcccgact ggaacggaag ccacaggatt
      721 cggcggctgc tgatggtggg caccccgaac cttggcacca tgcagccagt gttgtcccac
      781 gtcaggggcg aggagatcgg cctcaatgac attcccgcag aggtggtcgc cacctgctcc
      841 ggcgccccgc agctctttcc cgctccggag ctgacctggc tggtggatgc ccgcggcgag
      901 gacctgaacc tggacgtcta tgcgctggag acctggcgcg agcttgggtg gtccattttc
      961 agcgacaaag ccagagatcg ggcgacgagc aagcacggcg tgggccgccg gggccgggca
     1021 catctcgagc tgctcgagcg ctacctggcc aaacacctcg agcgcggaag gcgattccag
     1081 cacgctctgg cacgaccggc acggcccgac gagccggaac cattcgtctt cggcggcgac
     1141 tgcacgccaa cggtggcgcg gctggtgctg gaaaagcgcg gcggcgtatt cgtcggacgc
     1201 gagcaaccgg accgggtggc ggctcccgtg cgcggtgtgg actacgacgg tctcatcaac
     1261 gaacccggcg acggcgtggt gacgcgcgcc tcgctgacgg gccagaacgc aacgcccgtc
     1321 gccccccagc tgaacgtcgc gcactcggtg tttctgtgcg aggagcacca gcttctcacc
     1381 agcaacgcaa gctttcagga caacctgctc tacaatctcc tcaccgagtt cccccagggc
     1441 gcacacgggt gattccggga cgtcgccacg tcccggccgc atcccggcaa tcgtggattg
     1501 tccggagttc atgaacccga cacggccgtc tatactcgcc gtcgcgaaga gttgatcgac
     1561 gaggggagcg ggtcactggc cgtcacaacc tccattccgg cctccatgat gctgcgcctg
     1621 ctgatccgac cgagatcggt tttccgcctg ctcgcagatg cggatcccgc gccgctggtc
     1681 gtgttcttca agttcgcact ctggctcggc ctgcttccgc cactcttcgc ttacatcggc
     1741 gcgtccacgt tcggctggcg ggtcggcgtg gtggagccgc tgtttctgcc gcagccgacg
     1801 ctgatcgcga tcagcatcgc ctatttctgc tcgctgctct tcggcttctt cagcgcgtcg
     1861 atcatcagtc gctggatggc ggtgacctac ggcgcgcggg acagcctggg cgtgcatctg
     1921 gccatgatgg tcatcgtcgg cgcaccgctg gccatcgcca gcctgatgca tctgtatccc
     1981 aacgccctgc tgaacgccgt cgtgttcgtc ccgacgatga tctggagcat gtacctgctc
     2041 tacagaggac tgccgacgat cctgcacacc acccccgagc aggggatgct catggcctca
     2101 tcgatcatcg gctacctgct ggttgccgcc gtcagcctgc tcgggctcac cgtgttcttg
     2161 tgggtgatgg gcatcggtcc cgcccttgga gtctgatatt tgagcaccac gaacgatcag
     2221 acctacagcg accgggtcgt caccacgctc gttctgctcg ccatcgcttg gtcgatcgtc
     2281 gggatgaccg ccggcgtatg ggcggccgcg cagctcgtgt ggccgacact ggacttcgga
     2341 cagagctggt tgtcgtacgg caaggtgcgc accctgcaca ccaatgccgt catcttcggt
     2401 ttcggcgtga cggcgctgat gggcactgcg ttctacagtg ttcagcgcac gtcccacgtt
     2461 cgtctctttg caccgggtct ggcgtggttc acctgctggg cgtggcagat caccgtgttg
     2521 atcggcggcc tgtcgctgct ggcgggctac aacgccggta aggagtacgc cgagctggag
     2581 tggcctttgg acatcgcgat caccgtagcg tgggtgtgct tcgcggtggt cttcttcggc
     2641 accatcgcgc gtcgcaggat caagcccatt tacgtctcga actggtttta cggcggcctg
     2701 atcatcgtca tcgccatgct gcacatcgtg aacagcctgg ccctgccggt caccctgacc
     2761 aagtcctact ctctctacgc cggcgcccag gatgccatcg tgcagtggtg gtacggccac
     2821 aacgccgtgg gtttcctgct gaccggcggg ttcctcggca tgatgtatta cttcctgccc
     2881 aagcaggcag agcagccgat ctggagctac cggctgtcca tcgtggcctt ctgggcgttc
     2941 acgtattcgt acatatgggc gggccctcat caccttcact acaactccat ccccgagtgg
     3001 gtgcagtccc tgggcatggt gatgagcctg gtgctgctgg ccccgtcctg ggcgaccatg
     3061 gtcaacggca tcatgaccgt cagcggcgcc tacgagaagc tgcgcaccga tcccgccatg
     3121 aagttcatcg tgctctccct ggcgttctac ggcctggcca ccttcgaggg cccgatgatg
     3181 tccatcaaga gcatcaacgt ggtcagtcac ttcacggagt ggaccatcgg gcacgtccac
     3241 tcgggcgctc tgggctggaa cgccctgatc actttcggca ccttctactt tctggtgccc
     3301 cggttgatgg gcagcaagct ctacagcgtc aagctggcca actggcactt ctggctggcg
     3361 ctggcaggca ccatgctcta catcgtcgcc atgtggggcg ccggcgtctc ccagggcctg
     3421 ctgtggctga acatcgatga catgggcgag ctgcgctaca gcttcaccga catcatggcg
     3481 gccaccgtgc cctactacgt catccgcctt ctggccgggg ccgtattcct cgtcggggcg
     3541 ctgctgatgg cctacaacct ttaccggacc gtctccggca agcgcacgat cgaggtgcac
     3601 gcgccctcgg ccgacgacgc cgcaggagca tccgcatgag cggtatcggt ctgcatcgca
     3661 agctggagga gaactcgggt ctgctcattt tcgggattct gttcgtgtct gcgatcggcg
     3721 ggctggttca ggtactgccg agcctttttc aggaatccct caaaacgccc gcggcaaata
     3781 cccgcgtcta cacgcccatg gagctggtcg ggcgggacat ttacgtgcgc gaagcctgca
     3841 gcacctgcca ctcccagcag gtgcggccgc tctttgccga ggtccgccgc tacgggccct
     3901 acagccgctc gggcgagttc gtctacgacc gcccgtttct ctggggttcc aagcgcacgg
     3961 ggcccgatct tcaccgggtc ggtggcaagt acagcgacgc ctggcacgag atccacatga
     4021 tcgatccccg cgccgtggtg cccgactcca tcatgcccgc ctacccatgg ctcgcggaac
     4081 gggcagcgaa cgcggacggc gacatcgagg cgaagcttcg cggcctggtg acgctggggc
     4141 acccttacac cgatgaggac atcgccggcg cggcggcgag cctcgagggc gcgaccgagc
     4201 tcgacgcgct gatcgcctac ctgcagatgc tcgggaccgg gatggaggag acgccatgag
     4261 ttactttctg ttcgccggcg acatgttggt catggcgttc atgaccatcc tcgttgtctg
     4321 ggtatcgctg aagagttccg gcgatcagct ggaacgcgcc gcccgaatac cgctggagga
     4381 cgagcgtcac gatggctgac atgccctcgg atttctggag cggatggatc atcacgctga
     4441 ccgtggcgag cctggcgggg ctcgtctggc tcatcttcag cgtctatttc ggcaaggatc
     4501 ccgattcgga ccacacctcg ccggtatggg acgaaacgct ttcggagggc gccaacccgg
     4561 caccgatgtg gtggttctgg atgattctcg ccgccctggt cttttccgcc atttacctga
     4621 tgctgtaccc cggcctcggc agccacgccg ggatcatggc atggtcgcag ggtggcgctc
     4681 tggagaagag catgaaccgc ttcgatgcgg ccttcgccga gcggcgccag ggcgtcgtga
     4741 gcgccgagct ggcggatctg catgacgacg tcgatgccat gggcacggcc cggggcatct
     4801 tcacccgcaa ctgcgcggcc tgccacggtc cccagggact cggccaggcc aatgcctttc
     4861 ccaacctccg ggacacagcc tggcaatggg ggggcacgcc cgaggcgatc agccagtcca
     4921 tcacggccgg ccgccgcggc gccatggtcg gctggctacc cgtgctcggc gaggacggcg
     4981 tcgagcagct ggtgacttac gtcaaaaccc tttcagccgg cacgagcgat ccgaacgcgc
     5041 cggcggcaac gcagttcacg acctactgca gcgcctgtca cgcagccgac ggaaccggcg
     5101 tcgccatgct cggcggcccg gacctcaccg acgacgtgtg gctgtacggc ggctcggacg
     5161 acgcgctgcg gacgtccatc gccgagggcc gcaacggcat catgcccgcc ttcggcgatc
     5221 gcctggacga cgttcagctg aagctgctcg tcgcctggtt gacccgaccg cctaaggcct
     5281 cggcacgcac ggaaggcgcc gcccgctgat ccgaatcgcc agatgctcca aggaatcacc
     5341 cgagcatgaa tgagacagac cgtcgagcgc agatcgggct cgttgcggtc atcgggctga
     5401 ttctctggtt cggcctgaat cccttcgact tcgctcgaat caacaacgtc gagtggctcg
     5461 atccagggcc cggcctgcaa ttcggggact cgggactcgc tgtctccgag caagccttca
     5521 cgtggccgtc ggataccggt cccgcggcgg tgaccgttga aatgtgggtg cgcgcaagcc
     5581 gcgtttcgga caccgaacca agagcctggc tggtcctgat ggacgactcc aagctcaccc
     5641 cgctgggcgt acgtcaggaa gggggagacc ttgttgtctg ggacgcggtg acgaatccgg
     5701 atggcgaacg ctggtacaac gatttccggg ttccttacgc catgcgtccg ggtatcgagc
     5761 agcacatcgc catcacctcc cacgaagacg ggccactcat ctacatcgat ggttcgagtg
     5821 caggcaccta tgcgggctac cccattccgc tagcgcgcaa aggcgaaccg ttcggcggtc
     5881 ggctcgtcgt cggcgccggg ccaccctggg tcacgccgtg gatgggagaa gtgctcgggc
     5941 tcgccctata cgatcgcgcg ctgaccgcga cggaggtaga gcagcatgcg aacgcgggcc
     6001 cgggatccgg attcgccgaa ctggcaagga actcgggtgc cgttggcgcc tatcagttcg
     6061 acgcgggcgg cggcacctcg gttcaggatc aggcgggcgg ggggcacgac ctcaagatcc
     6121 ccgagtactt tcgcccaccg ggccgcgggc tgcttcggag cgtcgatccg gctgagcgca
     6181 cagcactctg gaacgtcggc gacgcgattc tgaacgttct gggctttgcc ccattgggct
     6241 tcttgatatc gattgtgcta cgcaagcacg tgaaaccgac ttccctcatt ctgaccgcaa
     6301 cgatggttgg tttcgtcgtc agcctaggca tcgagtggac acaatcgctg atgattacga
     6361 ggtcttcatc tttacaggat ctcgtgctga acaccgttgg cagcctggtc ggagccttgg
     6421 cggcccttgc cagttcccgt tttcgaggcc gcgaaaacaa taccaaagcg taacagagcc
     6481 agatgacacg gccggttgtt cgtcgagaaa gggattggta gctacggttg gacttgaacc
     6541 aacgacccca gcattatgaa tgctgtgctc taaccagctg agctacgtag ccatggcttg
     6601 cctgtgctgg cgaaggatcg cgattgtcct gacaagccga tgagctgtca acgtgcggcg
     6661 gacgcaggag gacggcgtgg acaaacccag aacggcgctg attaccggag catccgcagg
     6721 gatcggcaag gccttcgccg atgccctggc agcggggggt gtcaatctgg tgttggtggc
     6781 gcgacgagcc gatcggctgc agcaggcagc ccaggcgctg cgcgaagctc acgggatcga
     6841 cgtcgccacg atcagcgcag acctatccga tccgacggcc cccgtgcgca tacacgagga
     6901 gacggagagc cgtggcctgg tcatcgacta cctcatcaac aacgcggggg tagccgggcc
     6961 cgacctcctc gagccggata cctggcaggc caacgccggc ttccatcagc tgatgatgat
     7021 cgcgcccgcg cacctgtgcc atctgtacgt gccggccatg cgtgcccggc ggtgggggag
     7081 gatcgtcaac gttgcatcgg tggcggcgcg catacctcgc gccggcgggt gcaactacgg
     7141 accggcaaag gcctttctgg tggcgctctc agaggagctc tccctcaccc tgaaggctga
     7201 gggcatcaag gtgtgcgcgc tgtgcccggg cttcacgcac accgagtttc acaacgaagg
     7261 atccgcagca gagctcaagg cgaacacgcc cgccttcgtc tggtacgagg cggagacggt
     7321 ggtgaaggaa ggattacggg ccctcgagcg gggccgcagc gtctacgtgt ccggtcgcat
     7381 gtatcgctgg atggacccgt tctttcagtc ggtgtggacc cgccgactct tcaagatcgc
     7441 ggcgagctga acgcccgacc cggccggtgc ctcgaatcct gcgagccgac cccgagatca
     7501 gacattgaag cggaagagga tcacgtcgcc gtcctgaacg acgtagtcct tgccctctag
     7561 ccgccacttt ccggtgtcct tggcaccctg ctcgccgccg cagccgacgt agtcgtcgta
     7621 ggcgatgacc tccgcgcgga tgaatccctt ctcgaagtcg gtatgaatca cacctgccgc
     7681 ctggggcgcc cgcgtgccga tgcggacggt ccaggcgcga gcctccttgg gtccggcggt
     7741 gaagtaatgg tgcagcccga gcaggcgata gcctgcccgg atgacacgat tgagtcccgg
     7801 ctcttcgata ccgagatcct ccagaaactc cgctcgctcg tcttcttcga gctcgacaat
     7861 ctcggcctcg atcttgttgc agatgaccac cacctccgag gattcggcag cggcgatggc
     7921 ccgcacgccg tccagcaacg ggttgttgtc gaagccgtcc tccgccacgt ttgccacgta
     7981 gagaacgggc ttggccgtta tgaagtggca ctcgtgcagt aacttcagct cctcaggacc
     8041 gagcgccagc gcccgcaccg ggcgtcccgt attcaggccc ccgatgactt tgtccagcac
     8101 ggcgaccagc aacttggcgt ccttgtcgcc cgcgttggcg atgcgccggg tccggtcgaa
     8161 gacgcgctcc gccgtctcca gatcggcaag ggcgagctcc gtattgatga tctcgatgtc
     8221 gtcagcaggc gataccgcgc cggagacgtg ggtgacgttg tcgtcctcga agcaacgcac
     8281 gacgtgagca atggcatcgg tttctcgaat gtgcgcgagg aactgatttc cgagcccttc
     8341 accacgggac gcaccggcga caagaccggc gatatcgacg aactccatgc tggttggaac
     8401 gatcttggcc gaacgggcca gctgggcaac ccgctcgagc cgcggatcag ggacggggac
     8461 cacaccagta ttgggatcga tggtgcagaa cggaaagttc tccgcatcga tgcccgcctt
     8521 ggtcagggcg ttgaacagcg tggatttgcc gacgttcggc aacccgacga ttccgcactt
     8581 gaatcccatt cagtcctccc cttcgtcgcc cgcccccgcc ggcgcatgca gggcgttcat
     8641 ggcagcctgc agatccccgt cgagaatcgc cgccagcacc gcgtcttcca tacgccaggc
     8701 ggcttccatc tgaccccgtt ccgactcggg catgcgctcg gtagtgaggt aggcctcgac
     8761 acggcccctg tctccgggat ggcctacccc aatacgtaga cggggaaaac ccgcagcgtt
     8821 gccgagctga gagatgatgc tgcgcaaacc gttgtgccca ttgtcaccac cgcccgtcct
     8881 cagacgcagc atgcccggct cgaacgccat ctcgtcgtgg gcaaccagaa tttcgtccac
     8941 ggccagcttg tagaagcgcg ccaccgcagc caccgagctg ccactcagat tcatgtaggt
     9001 ggtgggaatg agcaggcgca catcatggcc gaggacctca ccgcggccca ttcggcccct
     9061 gaaacgcgcc tcgtcccgaa gcacgacgcc gaaccggtta gccaggtact cgacgaatcc
     9121 ggcacccgca ttgtggcgcg tgagctcgta ccgcgggccc gggtttccca ggcccacgat
     9181 caggctgacg tccgccatgg tcagtcttcg ctggcttcct cgccgccagc gccttcgtcg
     9241 gaggcttcgc cgccctcttc agcggccgct tccgcggcct cggcggcctc ttcctcttcc
     9301 agcccgccgc gcggcatctg gacgctgacc acgttggcat cacggtcgct gccgtgcatc
     9361 aaggcgacga tgctgacccc ttccggaagc gccagatcgg tgaggtgtac cgactgattg
     9421 acgtccacct gagccatgta gacctcgatg aactcgggca gatctccagg cagacagctg
     9481 atctcgacct cgttcatgtt atgcacgatc tgaccgccgc cctgcttcac gccgatgcac
     9541 ttgtcctcgt cgatgaagtg gagcggcacg gacacctgaa tgagacggtc ggcgcggatt
     9601 cgcagaaagt cgatgtgctg caccttttcc gaggccggat ggcgttgcag gtcccgtacc
     9661 accgcctgct caccctttcc gtccagaacg acgttcagga tctgcgagta aaaggcctcg
     9721 agctgcatgg ccttggaaag ctcgttgctg ctcagcatca gcgggaccgg atcagcatca
     9781 cccccataga ttacgccggg cactttgtct gcaaggcgac gcaggcggcg gctcgcacct
     9841 ttccccacgt cctcgcgcag ctcggcatga atttcaattc gctgcgtcat cagatttctc
     9901 catcaaatac ggacgtcccg gatcgcgacc aagtcctggc gtccaattgc aaccatcagc
     9961 tagcgaaaca tcgcgctgat ggattcctcg ttactcaccc gtcggatggc ttccgccaga
    10021 agacgggcga ggctgagctg ctcgatcttc gagcagcttt cggccgatcc gctcaacggt
    10081 atcgtgtcag tcacgaccat ccggtcgagc tcggagccgg cgattctttc gatggcgggt
    10141 cccgaaagaa ccgcatgcgt aatgtaggaa accacgccga tggcgccctc ttccttgagc
    10201 gcttcggccg ccgtgcacag cgtgcccgcc gtatcgacga tgtcgtcaac caggatgcac
    10261 gtcttgccgc ggacgtcgcc gatgacgttc atcacgcggg aatcgttcgc ctgcgggcga
    10321 cgtttgtcga tgatggcgag gtccagatcg ttgcactgct tggccaccgc gcgagcgcgc
    10381 accaccccgc ccacgtcggg tgagacgatg atgggattct cgtagtgctc accgagcata
    10441 tggtcgatca ggaccgggga cccgtaaacg ttgtccaccg gcatgttgaa aaagccctgg
    10501 atctggtccg cgtggagatc gacggtgagc atacggtcga tgccgacgct gccgagcatg
    10561 tcggcgacca ccttggcgct gatgggcact cgtgccgagc gggctcgcct gtcctgacgc
    10621 gcatagccaa aatacggtat caccgccgtc acgcgctgcg ccgatgcgcg gcggatggcg
    10681 tcggacatga tcatgagctc catgaggttg tcattggtcg gagcgcaggt ggattggatg
    10741 acgaagacgt cctgaccgcg cacgttctcg tggatctcca cattcacctc accgtcgctg
    10801 aacctcccca ggtcggcgtg cccgagatcc atccgcagct cctcggcgat gcgggcgctc
    10861 agcacggggt tcgagctccc cgtgaagatc atcaggtttg acaattggct tcccctcgtc
    10921 cgcgctcttt gcgcgtgcta tgcggttgcg gcttcaagag atcaacagtt tggctggggt
    10981 ggcaggattc gaacctgcga atgccgggat caaaacccgg tgccttacca cttggcgaca
    11041 ccccaacaga cagctcgttg ccatgtgcgc ccgccggcgc tgttcctgac gtttcgcggt
    11101 tctcgccccg gcagcggggc gctattgtga tgttgggtct gttggctgtc aaaccgccag
    11161 acctgacggg cagacgtcca cgtatcgggg cccgtcagag cacccagtcg gatatggaaa
    11221 gccgcacccg atagtcgccc cgcacagccg tgatccggcc cggcagccag cgggaaccac
    11281 gagccgtctg cacctggttg tgccgggcgt aggcgactga ccagtcgagc tgctcgaaag
    11341 cgaccaggcg cccatcatcg tcatactgcc ggccgccggc gccggttccc tctcccggct
    11401 tgcccagcac ccagctgggc agcacgtcca gtggaaggct ccagccaaga tgcgtacgca
    11461 tgacctcggc gtgaggcccc tcggagatca cggctccctt gccgtctaca atggccaggc
    11521 ggcgctcgtc cccatcgagc ctgacgcgcc cctgcccgag gggcccccag aggtcgatgg
    11581 tgaatgccga gccgtactgc ctccagacga agcgcgccga aaaactctcc gcgccttcga
    11641 cgacgccggc cttgccgcgc acctcgaagt cggccggggt ctcgacggga gggcggaacg
    11701 acgcgcagcc ggcgcagagg gccaaacaaa acaactttaa aatgaatggc ttaagtacgt
    11761 tcacaaatct ccgaagctgc tagaatgctg ggtcggcaag aaggctctca gctgagatca
    11821 taccctgcac aacatggcga tattggcata cggcattaac tatcgcacgg ctcccatcga
    11881 cgtccgggag cgcgtcgcat ttccggagga cgccctgccg gcggccctag aggatgccat
    11941 gtcgtctatt cccgatgtca gcgagatcgc catcatctca acgtgcaacc gcacggagct
    12001 catcgtctcc gcagcgcccg aggccgagga atccgtggcc cgttggctgg cgagctatcg
    12061 gccagtgagc atgggcgagc tgaacagcat ggcctacagc cactgggacc aggacgcagc
    12121 gcgacacctg attcgtgtcg cggcgggcct ggattctcag gtgctcggcg agccgcagat
    12181 catgggccag gtgaaacagg cttacgagct ggctagggcg gccggcgctc ttggttccga
    12241 gctgagcctg ctgtcccggc ttacgctcaa tgccgccaag cggatccgca ccgacaccga
    12301 tatcggccgc aaccccgtct cggtggccta cgctgcggtc accatggcgc agcagatctt
    12361 tgccgacctg caccacaaca aagcactgct catcggcgcc ggcgacacga ttcag
//
LOCUS       DBNHOX010000196         2746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1494803, whole genome shotgun sequence.
ACCESSION   DBNHOX010000196 DBNHOX010000000
VERSION     DBNHOX010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1494803"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="AC16UZ_04370"
     CDS             complement(<1..285)
                     /locus_tag="AC16UZ_04370"
                     /inference="COORDINATES: protein motif:HMM:NF037688.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245948.1"
                     /translation="MDRLAELFARVADSGVCTAATLREKFLACGIQPDDFRLRRTWQG
                     LDELAERPIDLETFRQLVSPELLMVARVLNKRLIVPEWQDFRQDIQYIYEQ"
     gene            complement(1142..1906)
                     /locus_tag="AC16UZ_04375"
                     /pseudo
     CDS             complement(1142..1906)
                     /locus_tag="AC16UZ_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700584.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(1910..>2746)
                     /locus_tag="AC16UZ_04380"
     CDS             complement(1910..>2746)
                     /locus_tag="AC16UZ_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6245949.1"
                     /translation="EGSITLGAAVAAALIASGTHPAVATLIAALAGFVAGTGTGLMTT
                     KCRIQPLLAGILAMTALYSINLRIMGRSNIPLINETTLATYVGRFGDALAGGGSGVNI
                     LGWTVGIRDLSILVGAFVVVVVISLFLYWFYRTQLGTAMRASGDNPQMMGALGVSVET
                     LTILGLAMSNGLIALSGALIAQYQGFADVQMGIGIIVIGLASVIIGEALVGARTVGLL
                     LCGAVMGSVLFRLLVAIALRWGLDPNDLKLVTAVFVFIALMAPTLIRRFGPSARLRSA
                     GA"
BASE COUNT          510 a          924 c          847 g          465 t
ORIGIN      
        1 ctgttcgtag atgtactgaa tgtcctgccg gaagtcctgc cattcaggca cgatcagccg
       61 cttattcagt acacgcgcca ccataagcag ctcggggctg accagctgcc ggaaggtttc
      121 gagatcaatc ggacgctccg ccagttcgtc caggccctgc caggtccggc gcagacggaa
      181 atcgtccggc tggatcccgc aggccaggaa tttttctcgc agcgttgccg ccgtgcagac
      241 ccccgagtct gcaacgcggg caaacaattc ggcgagacga tccatgaaag cgcctcacat
      301 ctgagaaagg cagtaaaggc ccttttctga gttggcgcaa acaccttcca gctcaggctg
      361 ccaatggtgt caggattcca aggctgaatc cagcaggtaa tctggctttg cacggcgcgc
      421 gcaggcacca ttcagctcca gcctcacttc gattctgaaa tcgcgaccgg gagcagcatt
      481 tacgtgcgtt cgggcggtgg tcagctttcc gaattcctcg gcctgccggc accccttgga
      541 tttgcccagg ggccggggct cagagctcga tgcgcgtggt gaagaagcct ttggccacgt
      601 agccttcgca caccatgtcg gcgtacttcg ccgagaagaa acgccccacg gagataacct
      661 tgccgccccg catgaaggtg ggctccgctt ccgggaccaa cgcgcgcgcc tcccccacct
      721 gcgcttcgtt ttgaggcacg atgaccacct gatgcctgcc ctggtcgctc gagctggtga
      781 cgtagcggat gaccatcgca cccacctcgt ccatgaccgt cgtctcttcc tcggtgaccg
      841 tctcgtctgc gtgcacgaag tgctggagca cgtcgagcag ctccgaggcc tgctcggtgg
      901 gcggagagac ccgcaggtat gccgctacgg cggcgcgcac cgccatgatg ccctcgcccg
      961 tcacgggggg agaagtgagg tccggcaggg gaacgttcca ccgctcggcg aattcccgga
     1021 ggagctccac ctcacgctcg tccacacgct cgtccacgta aatgaccgcc ttgaagactt
     1081 ccatcagctc ctcgatgccc tgtgcctcga ggaggagtcc ctccctcgac cgctcgagct
     1141 gctccttgcg gcgaaggtcc tcgaaacggc tcagcaggtc ctcggctctg atctgctccc
     1201 gggcggcccc ggagatgtcg tgcagcacct ggccctggtg catcatcacg atccggtcgc
     1261 ccagatccac cgcctgctgc atggagtggg tcaccatcag cgtggtgatc cgttcgcgcg
     1321 cgatgatctc gtgggtgagg cggatcacct gagccgcgct cttggggtcc aacgccgccg
     1381 tatgctcgtc cagaagaagc aggtccggct taagccaggt cgccatgagc agcgtgaggg
     1441 cctggcgctg cccgcccgag agggtgccga tggggttgtc caggcgattc tccaggccca
     1501 tgttcagaac gcgaatacgg tcccggatct cgtcccgaac gccggtcgtg agagccggcg
     1561 ccaaccctcg gctctgcccg cgacgcgctg cgagcgccag gttctccgcg atcgtcatgg
     1621 ccggcgcggt acccgagaag gggttttgaa acacccgccc gaccaggcgg gcgcgccgat
     1681 gctcgggcca gtcgctgatc gtctcccccg aaaggtggat cgagcccgag tccaccggaa
     1741 aggagccggc gacggcgttc agaatcgtcg acttcccgct gccgttcatg ccgatgatga
     1801 tcaggaacga cccatcggcg aggtcgatgg aaacgtccgt cagagcgcgc acctcgttgc
     1861 tggtgcccgc gtgaaacgtc ttcgagatct tctggatgct gagcatctgt caggcccccg
     1921 cggaccgcag tcgtgccgac ggcccgaagc gtctgatcaa cgtgggcgcc atcagcgcga
     1981 tgaacacgaa gaccgcggtg accagcttga ggtcgttggg atcaaggccc caacgcagcg
     2041 cgatcgccac cagcagcctg aagagcaccg aacccatgac ggcgccgcac agcaggaggc
     2101 cgaccgtgcg ggcacccacg agcgcttcgc ctatgatcac gctcgcaagg ccgatcacga
     2161 tgatgccgat ccccatctgc acgtccgcaa agccctggta ctgggcgatc aaggcgccgc
     2221 tcagggcgat caggccgttc gacatggcga gcccgaggat ggtgagcgtc tccacgctca
     2281 cgcccagggc acccatcatc tgaggattgt caccggaggc gcgcatggcc gtaccgagct
     2341 gggttcggta gaaccagtag agaaacaggc tgatcacaac cacgacgacg aaagcgccca
     2401 ccaggatcga aagatcccgg atcccgaccg tccaaccgag gatgttcaca ccggaacccc
     2461 cgccagccag cgcgtctccg aaccttccca cgtaggtggc cagggtcgtc tcgttgatca
     2521 ggggaatgtt gctgcgcccc atgatcctca gattgatcga gtagagggcc gtcatcgcca
     2581 ggatcccggc cagcagaggc tggatccggc atttcgtggt catcagcccc gtgcccgtgc
     2641 ccgcgacaaa cccggccaga gccgcgatga gcgtagcgac cgccgggtgg gtgcccgagg
     2701 cgatcaacgc ggcagccacc gcggcgccaa gggtgatcga cccctc
//
LOCUS       DBNHOX010000197         2633 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1173374, whole genome shotgun sequence.
ACCESSION   DBNHOX010000197 DBNHOX010000000
VERSION     DBNHOX010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1173374"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="AC16UZ_04385"
     CDS             complement(<1..576)
                     /locus_tag="AC16UZ_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6245950.1"
                     /translation="MAHLAVEGDRRIYFEHYPGAREVTVVLSHGWGMACRVWDNTTAC
                     LQDAGVGVVAYDHRNCGRSDKDFRDVSISALGDDVVALCDHLGLGGVVVNGWSLGGVV
                     VTDAAAKLGDRLAGLILTAAASPRYTQADGFPYGGQPEDVTATVAALRADRVNFLRTL
                     YYEGAYAKDVGEAVKNWCWQISLQASPGADAS"
     gene            complement(652..>2633)
                     /locus_tag="AC16UZ_04390"
     CDS             complement(652..>2633)
                     /locus_tag="AC16UZ_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6245951.1"
                     /translation="FILGLLTVWGAAGCSGPEDARDQATIEGPEAAVATESGIGGSED
                     AGEGAVLASTDEFQVGLGDLGDREALPGAALYADNCAACHDGSVPKAPLFTWLEMMSA
                     RTLLGAMNDGIMAAQAADLTADERVHIVEYLTRQRYVAGAVDDLADAHCEAPRAAFDR
                     DRPAPRVGWGHDTRRFVPGDVAGLSAADVPDLELAWAFVFPDALRARSQPAIGYGAVF
                     VGSQDGRVYALDLETGCVRWHYQAAAEVRTGIVVAEIHGRPMAFFGDILAWVHAVDAL
                     TGEPVWKRKADEHPGATLSGTPALHEGVLYVPVSSLEIIAAADPAYECCTFRGSVLAV
                     NAANGSERWRSYSIPEPPTVAQKTSAGTNVYAPSGAPIWASPAIDAKRNRLYVGTGEN
                     YSTPADGNSDAVLAIDLTTGERIWTRQMTEGDAWNVACMMADNPNCPPEDGPDFDLGS
                     SQMIVDLADGRQVLVGGHKIGAAFAFDLDAVKGDDPEPLWWTRVGRGSIQGGVHFGMA
                     ADGTTIYVPINDMNDTRNGDVLDPEAARPGVHAVNAADGKVLWSRVQPDRCGDDRPFC
                     DPGISAATTAVPGVLFAGHLDGVIRAYERDTGNVLWSFDSAREFTGVDGRVGRGGGMS
                     GAGPAVGEGYVVVNSGYGLYFHEPGNVLLAFRPSR"
BASE COUNT          443 a          945 c          863 g          382 t
ORIGIN      
        1 cgaggcgtcc gcccctgggc tcgcctggag tgagatctgc cagcaccagt tcttgaccgc
       61 ttcgcccacg tccttggcgt aagcgccttc gtagtaaagc gtacgcagga agttgacccg
      121 gtccgcacgc agcgcggcca ccgtggcggt cacgtcctcc ggctgaccgc catagggaaa
      181 accgtcggcc tgggtgtagc gaggcgaggc ggccgccgtg aggatcagtc cagccagccg
      241 atcaccgagc ttggctgcgg cgtcggtgac caccaccccg ccaagggacc agccgttgac
      301 gaccacccca cccagcccga gatgatcgca gagcgccacc acgtcgtcgc ctaatgccga
      361 gatggagacg tcgcggaagt ccttgtcaga gcgtccgcag ttgcgatggt cgtaggcaac
      421 cacgccgacg ccggcgtcct gaaggcacgc cgtggtgttg tcccataccc ggcaggccat
      481 tccccatccg tgagagagca ccaccgtcac ctcgcgggca cccggatagt gctcgaaata
      541 gattcgtcta tcgccttcta cggccagatg tgccacgact tcccccatca aaaaaccaag
      601 gctcatcgag cctaaggatc ctcggcattc agcgcaaccg gggctgcccg atcagcggga
      661 cggtcgaaag gccagcagca cgttgccggg ctcatggaag taaaggccat agcccgaatt
      721 caccaccacg tagccctcgc ccaccgcagg ccctgccccg ctcatgcccc cgccgcgacc
      781 aacccgcccg tccaccccgg tgaactcccg ggcggaatcg aagctccaca agacgttccc
      841 ggtgtcccgc tcgtaggcac gaatgacgcc gtcgagatgg cccgcgaaga ggacgcccgg
      901 gaccgccgtg gtggccgccg agatgcccgg gtcgcagaac ggccgatcgt ccccgcagcg
      961 gtcgggctgc acccggctcc acagcacctt gccgtccgcc gcgttgaccg cgtggacgcc
     1021 gggccgggcc gcctcgggat caaggacgtc gccgttgcgg gtatcgttca tgtcgttgat
     1081 gggcacgtag atggtggtgc cgtccgcggc catgccgaag tgcacgccgc cctgaatgct
     1141 gccccgcccc acccgcgtcc accaaagcgg ctccggatcg tcacccttca ccgcgtccag
     1201 gtcgaaggcg aaggcggcgc cgatcttgtg gcccccgacc agcacctgcc ggccgtcagc
     1261 caggtcgacg atcatctggc tggagccgag atcgaaatcc gggccgtcct ccggcgggca
     1321 gttcgggttg tcggccatca tgcaggccac gttccaggca tcgccttcgg tcatctgccg
     1381 cgtccagatg cgctcaccgg tggtgagatc gatggcgagc acggcatcgc tgttgccgtc
     1441 cgccggggtg gaatagttct cgccggtgcc cacgtagagc cggttgcgct tggcgtcgat
     1501 ggccggactg gcccagatgg gcgcgcccga aggggcgtag acgttggtgc ccgcagacgt
     1561 cttctgcgcg acggtcgggg gctcggggat ggagtagctg cgccagcgct cggagccgtt
     1621 ggccgcgttc acggccagca cgctgccgcg gaaggtgcag cactcgtagg cggggtcggc
     1681 ggccgcgatg atctccagcg aggagacggg aacgtacagg acgccctcgt gcagcgcagg
     1741 ggtacccgac agcgtggctc cggggtgctc gtccgccttg cgtttccaga ccggctcgcc
     1801 cgtgagcgca tccaccgcgt gaacccacgc gaggatgtcg ccgaagaagg ccatgggccg
     1861 accgtgaatc tccgcgacga cgatccccgt tcggacctcg gccgccgcct ggtagtgcca
     1921 gcgcacgcag cccgtttcca gatccagggc gtaaacccgg ccgtcctggc tgccgacgaa
     1981 gacggcgccg tagccgattg ccggctggga acgcgcgcgc agcgcgtcgg gaaagacgaa
     2041 ggcccaggcg agctccagat cgggcacgtc ggccgcgctc aggccggcga cgtcaccggg
     2101 aacgaagcga cgcgtgtcgt gcccccagcc aacccttggc gccggacgat cacggtcgaa
     2161 ggcggcgcga ggcgcttcac agtgggcgtc cgcaagatcg tcgacagcac ccgccacgta
     2221 gcgctgacgg gtcaggtact cgacgatgtg gacgcgctcg tcggccgtca gatcagcggc
     2281 ctgggcagcc atgatcccgt cgttcatcgc tccgagcagc gtgcgcgcgg acatcatctc
     2341 cagccaggtg aacaggggcg ccttgggcac gctgccgtcg tgacaggccg cgcagttgtc
     2401 ggcgtaaagg gccgcgcccg gcagcgcctc gcggtcaccc agatcgccga gccccacctg
     2461 gaactcgtcg gtagacgcca gaacggcccc ctcgccggcg tcctccgagc ccccaatccc
     2521 ggactcagtc gccaccgcgg cttccggacc ctcgatcgtc gcctgatccc gggcatcttc
     2581 gggccccgag cacccggcag cgccccagac cgtcagcaga cccaagatga aaa
//
LOCUS       DBNHOX010000198         2902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_402050, whole genome shotgun sequence.
ACCESSION   DBNHOX010000198 DBNHOX010000000
VERSION     DBNHOX010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_402050"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="AC16UZ_04395"
     CDS             <1..427
                     /locus_tag="AC16UZ_04395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6245952.1"
                     /translation="LLRHILVMPSEIRSEEEAEALIRQVHDELEAGGDFVALAAEHSE
                     DPGSALNGGDLGWTSGNEFVPAFREAMYATTTGELSEPFRSQYGWHVLEVQERREQDL
                     SEETRRNMAMQILHQRRFDEELQEWLKELRDEAFVEMRL"
     gene            433..1413
                     /gene="pdxA"
                     /locus_tag="AC16UZ_04400"
     CDS             433..1413
                     /gene="pdxA"
                     /locus_tag="AC16UZ_04400"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR6245953.1"
                     /translation="MPISLAITPGEPAGVGPELLLRLAESPRTRPWVAVADADLLAER
                     ARALGTTVRLDDAWDRPAMEAGRLTVRHVPLEAPCTPGQLDPRNAAYVLETLRLATDG
                     CLSGEFAGLVTGPVQKSVMNDAGIPFTGHTEFLCERAGVADVLMLLVAGTLRVGLATT
                     HLPLREVPDALSRALLEGRLGLLLDGLRRDFGIARPRVLVAGLNPHAGEGGHLGTEEI
                     EIIDPVCERYRAAGEQVKGPLPADTLFTPAMLDTADAVLAMYHDQGLPVLKHAGFGRA
                     VNVTLGLPFVRTSVDHGTALDLAGSGVADPGSFAAAASLAGDLLDFRASA"
     gene            1460..2176
                     /gene="rsmA"
                     /locus_tag="AC16UZ_04405"
     CDS             1460..2176
                     /gene="rsmA"
                     /locus_tag="AC16UZ_04405"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003206170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR6245954.1"
                     /translation="MADLIRLGPHDQVLEIGPGTGALTAALLDASGAAPARYLAVEID
                     RDLAPELGRRFPAVEVVNADVLRVPLDELLGSQSWRVVGNLPYNISTPLLVRLFACTD
                     RIRDMHFMLQREVALRLAAVPGTKAWGRLTVLAQYHCQVELLFEVAPESFSPPPKVWS
                     AVARLVPHAAKLPLENPDLLDLVLRHAFSGRRKRLGNALKSLSMPWHELDVDDAARPD
                     QLSVKDFVTLANAVQLEQTP"
     gene            2173..2553
                     /gene="apaG"
                     /locus_tag="AC16UZ_04410"
     CDS             2173..2553
                     /gene="apaG"
                     /locus_tag="AC16UZ_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HIR6245955.1"
                     /translation="MSGPASGIEVAVDARYVVGESDPIRNRYVFAYTITISNLGPEPG
                     QLLDRHWFIADGDNEVREVQGPGVVGEQPRLEPGTAFRYTSAAVLETPVGTMHGLYGF
                     RRDDGTRFEVPIPMFSLAAPNAVH"
     gene            2563..>2902
                     /locus_tag="AC16UZ_04415"
     CDS             2563..>2902
                     /locus_tag="AC16UZ_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6245956.1"
                     /translation="MATYAIGDVQGCYAEFCRLLKKMEFDKKRDRLWMVGDLINRGPD
                     SLRVLNLVRSLGKRAVIVLGNHELHFLAIHYGGYSANRTDTFDDLLSSPARAEIAEWL
                     CQQPLLVRSNK"
BASE COUNT          436 a          979 c          991 g          496 t
ORIGIN      
        1 gcttctgcgt cacatcctgg tcatgccctc ggagatccgc tcggaagagg aagccgaagc
       61 gctcatccgt caggttcacg acgagctcga ggccggcggc gatttcgtcg ccctggcggc
      121 cgagcactcg gaggatccgg gcagcgcgct caacggcggt gacctgggct ggacgtcggg
      181 caacgagttc gtgcccgcgt tccgcgaggc gatgtatgcg accacgacgg gcgagctgtc
      241 cgaaccgttt cgctcccagt acggctggca cgttctggag gttcaggagc gacgcgagca
      301 ggatttgagc gaagagaccc gccgcaacat ggcgatgcag atcctccatc agcgccgctt
      361 cgacgaggag ctccaggagt ggctgaagga actgcgggac gaagcgttcg tggagatgag
      421 gctctagcgg gtgtgccgat aagcctcgcc atcacgcccg gcgagccggc gggcgtcggc
      481 ccggagcttc tgctccgact ggcggaaagt ccccgcacgc gcccctgggt agcggtggcc
      541 gacgcggatc ttctggccga gcgggcccgc gccctgggca ccacagtccg gttagacgac
      601 gcctgggacc gcccggccat ggaagccggg cggctgaccg tgcggcacgt gcctctcgag
      661 gctccttgca cccccgggca actcgatccc cgcaacgccg cctacgtcct ggaaaccttg
      721 cggctggcga cggacggttg cctgtcgggg gagtttgccg gccttgtcac gggacccgtg
      781 cagaagtcgg taatgaacga cgcgggcatc ccgttcaccg ggcacacgga gttcctgtgc
      841 gagcgggcgg gcgttgccga cgtgctcatg ctgctggtgg cgggcacgct tcgggtcggg
      901 ctcgccacga cccatttgcc cctgcgcgag gtgcccgacg ccctttcccg agctctgctc
      961 gagggccggc tcggcctgct gcttgacggg ctgcgaaggg atttcggtat tgcccgaccc
     1021 cgggtactgg tcgccggttt gaacccccac gcgggggagg gcgggcacct gggtaccgag
     1081 gagatcgaga tcatcgatcc cgtgtgcgag cgctaccgcg ccgccgggga gcaggtgaag
     1141 gggccgctgc ctgccgacac cctcttcacc ccggccatgc tcgacaccgc agatgcggtg
     1201 ctcgccatgt atcacgacca gggtcttccg gtgcttaagc acgccgggtt cgggcgggcg
     1261 gtcaacgtca cgctcggcct tcccttcgtg cggacgtcgg tcgaccacgg taccgcgctt
     1321 gacctggcgg gctccggggt ggctgacccg ggcagctttg ccgccgccgc ctcgctggcg
     1381 ggcgacctgc tggatttccg tgcgagcgcg taagcggttc ggccagcact ttctcaccga
     1441 cgaagcggtg ctgcagcgca tggcggatct gatccgtctc gggccgcacg accaggtgct
     1501 ggagatcggt ccgggcaccg gggcgctcac cgccgctctg ctcgacgcct ccggagcggc
     1561 gccagcgcgt tacctggcgg tggagatcga ccgcgatctg gcccctgagc tcgggcgccg
     1621 cttccccgcc gtggaggtcg tcaacgccga cgtgctgcgc gttccgctcg acgagctgct
     1681 gggctcgcag agctggcgcg tggtgggcaa tctgccctac aacatctcca cgccgcttct
     1741 ggttcgtctg ttcgcctgca ccgaccgaat ccgcgacatg cacttcatgc tccagcgcga
     1801 ggtggcgctg cgcctcgcgg ccgttccggg caccaaggca tggggccggc tgacggtgct
     1861 cgcccagtac cactgccagg tggagctgct cttcgaggtt gcgccggaga gtttcagccc
     1921 accgccgaaa gtctggtccg ccgtcgcgcg gctggtgccc catgcggcca agctcccgct
     1981 cgagaacccg gacctgctgg atctggttct gcgccacgcg ttctccgggc ggcgcaaacg
     2041 gctcggcaat gcactaaaat ccttgagcat gccctggcat gaactcgatg tggatgacgc
     2101 cgcccggccc gaccagctgt cggtgaagga tttcgtcacg ctcgccaacg ccgtgcagtt
     2161 ggagcagaca ccgtgagcgg acccgccagc ggcatcgagg tggcggtgga tgcgcgctac
     2221 gtcgtcggcg agtcggatcc gatccggaac cgctacgtgt tcgcctacac cattacgatc
     2281 agcaacctcg ggccggagcc cggtcagctc ctcgaccgcc actggttcat cgccgacggc
     2341 gacaacgagg tgcgcgaggt gcaggggccg ggagtggtcg gcgagcaacc ccggctcgag
     2401 cccggcaccg ccttccgtta taccagcgcc gcggttttag aaacgcccgt gggaaccatg
     2461 cacgggctct acggcttccg ccgtgacgac ggcacgcgct tcgaggtgcc gattcccatg
     2521 ttctcgctag ccgcgcccaa cgcggtgcat tgagaccggg cagtggccac ctacgcgatc
     2581 ggtgacgttc agggttgcta cgcggagttc tgccggctgc tcaagaaaat ggagttcgac
     2641 aagaagcgcg atcggctctg gatggtgggt gatctgatca accgtgggcc cgattcgttg
     2701 cgggttctga acctggtgcg ttccctgggc aagcgcgcgg tcatcgtgct cggcaaccac
     2761 gagctccatt ttctcgccat ccattacggc ggctacagcg ccaaccggac cgacacgttc
     2821 gatgatctgc tgagctcccc ggcccgcgcc gagatcgccg agtggctgtg ccagcagccg
     2881 cttctggtcc ggagtaacaa gc
//
LOCUS       DBNHOX010000199         2103 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_530853, whole genome shotgun sequence.
ACCESSION   DBNHOX010000199 DBNHOX010000000
VERSION     DBNHOX010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_530853"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1721)
                     /gene="dnaE"
                     /locus_tag="AC16UZ_04420"
     CDS             complement(<1..1721)
                     /gene="dnaE"
                     /locus_tag="AC16UZ_04420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR6245957.1"
                     /translation="MKVGALADAARRLNMPAVALTDRSNLFGLVKFYQACLSAGVKPL
                     VGSDLVYQTDEGGRFRIAVLAMNSQGYRNLLALVSAAYLDTDERGVLSAGQIFARQEG
                     LLVLSGGRQGEVGQALAKGDLPGAQRIAALWEERFPERFYLELVRTGRGGEDAYIDGA
                     VRLAAELELPVVATNDVCFLAADDFEAHETRVCIYDGRTLDDPRRERSYSSDQYLRTP
                     AEMTALFSDLPEAIENTVQIARRCNVQIELGDYHLPNYPVPEGETLGGFLQRCAREGL
                     ERRWQTLAGRPEQAFSFEEYRERLSYELDVINQMGFAGYFLIVMEFIAWAREHGIPVG
                     PGRGSGAGSLVAYALGITDLDPLGYDLLFERFLNPERVSMPDFDIDFCMEGRDRVISH
                     VSERYGHDAVSQIITFGTMAAKAVVRDVARVQGKPYGLADKLSKLIPFEVGMTLEKAV
                     SQSSELADFIASSEDVGEIMDMAYKLEGIVRGLGRHAGGVVIAPSALTDFVPLYMDDQ
                     SGGLVSQFDKDDVESAGLVKFDFLGLKTLTIIDWAVQAVNAARGDADELLDIDHIPLD
                     DPGTFEFL"
     gene            complement(1799..>2103)
                     /locus_tag="AC16UZ_04425"
     CDS             complement(1799..>2103)
                     /locus_tag="AC16UZ_04425"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR6245958.1"
                     /translation="QVDGNRRPPGLPQAEAIVGGDALIPAISAASIVAKVARDAELTA
                     LDAQYPGYGLARHKGYPTAEHLSALAELGACAVHRTSFAPVRDLKLRRPTVERLPL"
BASE COUNT          380 a          705 c          669 g          349 t
ORIGIN      
        1 aggaactcga aggtccccgg gtcatccaga gggatgtggt cgatgtccaa cagctcgtct
       61 gcgtctcccc gcgccgcgtt cactgcctgc acggcccagt cgatgatggt cagcgtcttc
      121 aggccgagga agtcgaactt gaccaggccc gcgctctcca cgtcgtcctt gtcgaactgc
      181 gaaactaacc cgccgctctg atcgtccatg tacaggggga cgaagtccgt cagcgccgac
      241 ggcgcgatga ccacgcctcc ggcgtggcgc cccaggcccc gaacgattcc ttccagcttg
      301 taagccatgt ccatgatctc acccacgtcc tcgctggaag cgatgaaatc cgcaagctcg
      361 ctgctctgcg acactgcctt ttccagcgtc atgccgactt cgaacgggat cagcttggag
      421 agcttgtcgg ccagaccgta gggcttgccc tgaacccggg cgacgtcccg aaccaccgct
      481 ttagcagcca tggttccgaa ggtgatgatc tggctgacgg catcgtggcc gtagcgctcg
      541 gacacgtggc tgatgacccg gtcacggccc tccatgcaga agtcgatgtc gaagtccggc
      601 atggaaaccc gttccgggtt gaggaatcgc tcaaacagaa ggtcgtaacc cagggggtct
      661 agatccgtga tgcccagcgc gtaggcgacc agcgagcctg cgccggagcc gcgcccgggc
      721 cctacgggaa tgccgtgctc gcgcgcccag gcaatgaact ccatgacgat caggaagtag
      781 ccggcaaaac ccatctgatt gatcacgtcg agctcgtagc tcaggcgctc ccggtactcc
      841 tcgaacgaaa aggcctgctc gggacgaccc gccaatgtct gccatcggcg ctcgaggcct
      901 tcccgcgcgc agcgctggag gaatcccccc agcgtctcgc cttccgggac cgggtagttg
      961 ggcaggtgat agtcgccgag ctcgatctga acgttgcagc ggcgggcaat ctgaacggtg
     1021 ttctcgatcg cttcaggcag gtcgctgaag agagccgtca tctccgcggg cgttcgcaga
     1081 tactggtcgg acgagtagct gcgttcacga cgcggatcgt ccagcgttcg gccatcgtag
     1141 atgcagaccc tcgtctcgtg agcctcgaag tcgtccgcgg ccagaaagca gacgtcattg
     1201 gtagctacca cgggcagctc gagttccgcc gccagccgca ccgccccatc aatgtaagca
     1261 tcctcgccgc cgcgaccggt gcggacgagc tccaggtaga agcgttcggg aaagcgctcc
     1321 tcccacaggg ccgctatcct ttgagcccca gggaggtcgc ccttggcaag agcctggccc
     1381 acctcaccct ggcgcccgcc ggaaagcacc agcagcccct cctgccgggc gaatatctgg
     1441 ccggcgctga gcacgccgcg ttcgtcggta tcgaggtagg cggcggaaac gagggccagc
     1501 aagttgcgat atccctggga gttcatggcc agaacggcta tccggaaccg gccgccttca
     1561 tccgtctggt agacgagatc cgagcccacc aacggcttca cgcccgcgct gagacaggcc
     1621 tggtagaact tcaccagccc gaacaggttg ctccggtcgg tcagggcgac tgccggcatg
     1681 ttcaagcgcc gcgcggcatc agccagggcg cccactttca tcaggccatc ggcgaccgag
     1741 aactcgctgt gaacccgcag gtggacgaag ccttcagtgc ttgccggggt tacggcggtc
     1801 acagcggaag tcgctccacg gtcggccgac ggagcttgag atcgcggacc ggcgcaaagc
     1861 tcgtccggtg gacggcacac gcacccagct ccgcgagcgc cgagaggtgc tcggcggtcg
     1921 gatagccctt gtggcgggcc aggccgtagc ccggatactg cgcatcgagg gccgtcagtt
     1981 cggcgtcgcg cgccaccttg gcgacgatgg acgcggcgct gatggccggt atcagcgcat
     2041 cgccgccgac gatggcctcg gcctgcggca ggccgggcgg gcgacggttg ccgtccacct
     2101 gaa
//
LOCUS       DBNHOX010000200         2609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1751940, whole genome shotgun sequence.
ACCESSION   DBNHOX010000200 DBNHOX010000000
VERSION     DBNHOX010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1751940"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..561
                     /locus_tag="AC16UZ_04430"
     CDS             103..561
                     /locus_tag="AC16UZ_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009681892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR6245959.1"
                     /translation="MDRIDRAILRELQNDARLPNTALAERVGLSDSACLRRVRRLEQS
                     GVIRGYVALVDETLAGYPDSVFVQITLVSQQRKDLEAFEDAVARVPEVMECYLMSGAS
                     DYILRVVVADARDYERIHSQQLTRLPGVDRVHSNFALRTVIENTEIPIRT"
     gene            704..2254
                     /locus_tag="AC16UZ_04435"
     CDS             704..2254
                     /locus_tag="AC16UZ_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR6245960.1"
                     /translation="MEISDQSQLALIALCIYFVVVVGLGLFYSRKSQSTEDFILAGHS
                     LTTPFVMGSVVATWMGGAVILGGAKEAFVGGFQAIVWDPWSPMLTLILCGFFFVSVFR
                     RSRFTTSIDFYNARYNRGIGLAALSISMVAYISWISAQLLSLGVIIKVVTGMDAVMAT
                     LAGSMIVLVVSLSGGLWALSRSDMLAFIILSVVLILVLPLALDAVGGPAAFIENAGTR
                     DGSPPFSLFYTEAPTAAGEPTGFYGYLGMLGVFYMLAAWFSVAIGDLGGSVLTARALA
                     AKDEVSATRGFLFGGVIYLLLGMIPVIVGMCVFILSPDIAEADLDDVLPIFVQQYLPD
                     WITVLFFVAVASAIVSTAGDTILTGGALLGYTALGAVKPESSDRTRLRATRTAMVLFT
                     AIALVFGLAMGDLYRLLVFAGAVAFPVTSAAFVCGVLWKRANVPGAWASIAAGTVSWV
                     IFVVLLLPEVDWEVWDALYIGAVPAFLCSAIAIVVVSLATRRTHPPNPVRDVDGNDIS
                     GGPLFRWR"
BASE COUNT          414 a          780 c          837 g          578 t
ORIGIN      
        1 ctagctgaac ggcgaggccg ggttattgca ttcttgctct aatttagccc tttggcacaa
       61 tatataagtc atatactgga aaatataagc agaatattgc atatggatcg aattgatcgc
      121 gcgattctgc gggagcttca gaacgatgcc cggctgccga acaccgccct ggcggaacgg
      181 gtcgggttgt cggactccgc gtgtctgcgc cgtgtgaggc gtctcgagca atcgggcgtg
      241 atccggggct acgtggcgct ggtcgacgag accctggccg gttacccgga cagcgtgttc
      301 gtgcagatca ccctggtcag tcagcagcgg aaggatctgg aggcattcga ggatgcggtg
      361 gccagagttc cggaggtaat ggagtgctat ctgatgtcgg gcgcctcgga ctacatcctg
      421 cgggtcgtgg tcgcagatgc ccgggactac gagcgtatcc acagccagca gctgacgcgg
      481 ctgccgggcg tggaccgggt gcactcgaac ttcgcgctgc gcacggtcat cgagaacacg
      541 gagataccga ttcgcaccta gccaagcgtt aggggtttcc actagtggtc cagagtaaga
      601 tcgcactagc agaatcggtg agggtggcag attgatgctg gattcgccgg ggggataagt
      661 cggccgcggc gtgcttcgac gacggggagg cggatgagtc gggatggaaa tttctgacca
      721 atctcaattg gcgctgatcg ccctgtgcat ctacttcgtc gtcgtcgtgg ggctgggcct
      781 cttctattcc cgaaaaagcc aatcgaccga agatttcatc ctggccgggc acagcctcac
      841 gacgcccttc gtcatgggct ccgtggttgc gacctggatg ggcggagcgg tgatactcgg
      901 tggggccaag gaagccttcg tcggaggctt tcaggccatc gtctgggatc cgtggtcgcc
      961 catgctgacc ctgattctgt gtggtttctt ctttgtcagc gtgttccggc gttcccgctt
     1021 caccacttcc atcgacttct acaatgcccg gtacaaccgt gggatcgggc ttgcggcgct
     1081 gagtatcagc atggtggcgt atatctcctg gatatcggcg cagctgctct ctctcggtgt
     1141 gatcatcaag gtcgtaaccg gaatggacgc ggtcatggcg actctggctg gcagcatgat
     1201 cgtgctcgtc gtttcgctgt ccggcgggct gtgggcgctg tcccgatccg acatgctcgc
     1261 cttcatcatc ctttccgtgg tgctgatcct ggtgctgccc ctggcgctgg atgcggtcgg
     1321 tggtcccgct gctttcatcg agaacgcggg caccagggat ggatcgccgc ccttcagctt
     1381 gttctacacc gaggcgccaa ccgcggctgg cgaaccgacg ggcttctacg gctacctggg
     1441 catgctcggc gtcttctaca tgctggccgc ctggttctcg gttgccatcg gcgatctcgg
     1501 cggctcggtg ctcaccgccc gggctctggc ggcaaaggac gaagtctcgg caacgcgggg
     1561 ctttcttttc ggcggggtca tctacctgct gcttgggatg attccggtga tcgtgggcat
     1621 gtgcgtcttc atcctgagtc ccgacatcgc agaagccgat ctggacgacg tgcttccgat
     1681 cttcgtgcag caatacctgc cggattggat cacggtgctg tttttcgttg ccgtggcgtc
     1741 cgccatcgtc agcacggccg gggataccat cctgactggc ggcgccctgc tgggctacac
     1801 ggcgctcggt gccgtcaagc cggagagctc cgatcggacc agactgcgcg caacgcggac
     1861 cgcgatggtt ctgttcacgg ccatcgcgct cgtgttcggc ctcgccatgg gcgacctcta
     1921 ccggctgctg gtgtttgcgg gcgcggtggc gttcccggtc acctcggcgg ccttcgtatg
     1981 cggcgtgctg tggaagcggg ccaacgtccc gggcgcctgg gcgtcgatcg ctgctggcac
     2041 cgtcagctgg gtgatcttcg tcgtgctcct tctgcccgag gtcgactggg aagtctggga
     2101 tgccctctac atcggcgcgg tccccgcttt cctttgcagc gcgattgcca tcgtggttgt
     2161 gtccctcgcc acgcgcagga cccacccgcc caatccggta cgcgacgtgg atggaaacga
     2221 catttccggt gggccgctgt tcagatggcg atgacgaggc tgcgcaaacg cgcgcggctc
     2281 ggcagtcccc ccgccgcgac cgcctacgaa agctgccgct gcgtatcctt atctcctcgt
     2341 caggttgttg gccggttcct ctgatgccgg tgggtcacgc cagccgatgc cactaagtgg
     2401 aaccccgaat caccaaatcc cagtcagacc cgcatcatca gagactagac caatacgtgg
     2461 agatgccatg ggaatcccga tccgagttct gatcgttctg ctgtctgctc ttgccattgc
     2521 cggttgccag actgccgcgc cggagggtga acggtctggc tacgagtacg atggcagaaa
     2581 cctctatctg ggttactgcg cgagctgcc
//
LOCUS       DBNHOX010000201         3109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_16012, whole genome shotgun sequence.
ACCESSION   DBNHOX010000201 DBNHOX010000000
VERSION     DBNHOX010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_16012"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..327)
                     /locus_tag="AC16UZ_04440"
     CDS             complement(160..327)
                     /locus_tag="AC16UZ_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245961.1"
                     /translation="MSLISSVLRPVFYTMEDFMEQTLVMKAVIAWGWLSVIVMVVSFV
                     ATLPELLGAGG"
     gene            complement(355..1680)
                     /gene="rimO"
                     /locus_tag="AC16UZ_04445"
     CDS             complement(355..1680)
                     /gene="rimO"
                     /locus_tag="AC16UZ_04445"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR6245962.1"
                     /translation="MTGKVGFISLGCPKALVDSEQLLTALTAEGYTTADDYSEADVVV
                     VNTCGFIDAAIEESFDAIEEALDACGKVVVTGCLGARKDSLQERYPELLSVSGPQDPV
                     PVVDAVRAHVPLSAAESTHYHLGPAGVKLTPAHYAYLKVAEGCNHTCSFCIIPSMRGK
                     LRSRALPDLLEEADRLAEVGVEELLVISQDTSAWGTDLRRSELRGLKPGEALELLCRE
                     LAARFPWVRLHYLYPYPHVDQLLDLMAEGCLLPYLDVPLQHADPAILKAMRRPAAAQN
                     TLDRLARWRDRCPEVSVRSTFIVGFPGEGQREFETLLEFLEAAELDRVGCFAYSNVAG
                     AGANNLPDQVPEPVKQERIERLMTLQARISREKLARKVGQELEVIVDAVEDSLALGRT
                     RFDAPDVDGVVRVHGGRSLVPGERTWVRITGSDTHDLDADYVGNPLKFA"
     gene            2050..>3109
                     /locus_tag="AC16UZ_04450"
     CDS             2050..>3109
                     /locus_tag="AC16UZ_04450"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003382491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR6245963.1"
                     /translation="MMSAELENLNVAETEVLVTPAALKALLPVPDAVGESVRASRRAV
                     QNILDHSDHRLLVVVGPCSIHDTDAAREYAQRLKQLADEVSSELFVVMRTYFEKPRTT
                     VGWKGLINDPYLDDSFRVGEGLEIARRLLLDITSLGLPVSTEALDPITPQYLQDLITW
                     SAIGARTTESQTHREMASGLSCPIGFKNGTDGSLDVAINALESAVSPHRFLGISSSGQ
                     VSVIRTTGNRHAHIVLRGGSRGPNYDAENVAACEAALEKIGQVPNIMVDCSHANSNKD
                     HRRQPDVARGVAEQIENGSRSLMGLMIESNLHEGNQPVNGGSNLRYGVSVTDACIDWP
                     TTESLLRELAERLGPALRA"
BASE COUNT          588 a         1030 c          971 g          520 t
ORIGIN      
        1 ctccgtgccc ggatagagct ccatctcacg cttgaactcg tcgagcatcc cctcttcgtt
       61 cggatccaac aggccatcgg aaaccatgac gttgtgagcg agccccagaa gggcttcgcg
      121 ctgctgggga ttcaggaaat gaagccacac ggcttcgcgc tagcccccgg cgccgagcag
      181 ttcgggcaga gttgctacaa agctcaccac catcacgatc acggacagcc aaccccaggc
      241 gatgacggcc ttcatgacca gggtctgctc catgaagtcc tccatggtgt agaacacggg
      301 tcgcaggacg gacgaaatca ggctcatact cggctccagc aaactgcgcg cagtttaagc
      361 gaacttgagc ggattgccca cgtagtcggc atcgaggtcg tgggtatcgc taccggtgat
      421 tcgcacccag gttctctcac ccggtaccag cgatcgtcct ccatggacac gaaccacgcc
      481 atccacgtcc ggcgcatcga atcgcgttcg cccgagcgca agtgagtcct ccaccgcatc
      541 cacgatcacc tccagctcct gccccacctt gcgcgcaagc ttctcccggc tgatccgggc
      601 ctgcagcgtc atgagcctct cgatccgctc ctgcttgacc ggttccggaa cctgatcagg
      661 caggttgttg gctccggcac ccgccacgtt cgagtaggcg aagcacccca cccgatccag
      721 ctcggccgct tcgaggaact cgagcagagt ctcgaactcg cgctgcccct ctccgggaaa
      781 cccgacgatg aacgtgctgc ggacgctgac ctcaggacag cgatcccgcc agcgagccag
      841 ccgatcgaga gtattctgcg cggctgccgg gcgacgcatg gccttgagga tcgccggatc
      901 cgcgtgctgc agcggcacgt ccagataagg aagcagacac ccctccgcca tgaggtcgag
      961 gagctgatcc acgtgggggt aagggtagag atagtgcaga cgaacccagg ggaaacgggc
     1021 tgcgagctcg cgacacagca gctccagggc ctcgccgggc ttgagcccac gcagctcgct
     1081 ccggcgcagg tcggttcccc acgcgctcgt gtcctgagag atgaccagca gttcctcgac
     1141 accaacctca gcgagacgat ccgcctcctc cagcaggtcc ggcagcgctc gagagcgcag
     1201 cttgccccgc atgctcggaa tgatgcagaa gctgcaggtg tggttgcagc cctcggcgac
     1261 cttgagatag gcgtagtgcg cgggagtcag cttcacgccg gcggggccaa ggtggtagtg
     1321 cgtggactca gcagcgctca gcgggacatg ggcccgcacg gcgtccacga cgggaaccgg
     1381 gtcctgagga ccgctgaccg acaggagctc cgggtaacgt tcctgcaggc tgtctttccg
     1441 cgcgccgagg cagccggtca ccaccacctt gccgcaggcg tccagggcct cttcgatggc
     1501 gtcaaacgac tcttcgatgg ccgcgtcgat gaaaccgcag gtattgacga ccaccacgtc
     1561 ggcttcggag taatcatcgg ccgtggtata cccctccgcc gtcaacgccg tgagcagctg
     1621 ctccgagtcg acgagggcct tgggacatcc gaggctgatg aaccccacct ttcctgtcat
     1681 cttctagttg cccggcaccg gttgtgaagc gtcgcgcatt gtagccgtgg ccggtcgtcg
     1741 ggtcactgaa ccactcgaga tggacgatgc aggcgcatgc agacgcaccg agcgcgagtt
     1801 gattttcccg gtcgagtgtg taaccgtccg cgcgccatgg aagacaacgc acgacaattc
     1861 tggctccagg tctggtccca aggctccaac gccgttggcg gcacgggcag ccagggttgg
     1921 tattggtttt ttaacggata gcaccgcctt ctagaaaagg gaggcagtgc aggctgcctt
     1981 cgcagggaac cccgcaaggc agcgccagcg gggttttttt tgccctgatg aaggcgtttc
     2041 gaggagacga tgatgtcggc agaactagag aacctgaacg tcgcggagac ggaggtgctg
     2101 gtcacccccg ccgcgctcaa ggcgctgttg ccggtgcccg acgcggtcgg cgagagcgtg
     2161 cgggcatccc gtcgggcggt gcagaacatt ctcgaccact cggatcaccg gctgctggtc
     2221 gtggtcggcc cctgctccat ccacgacacg gacgccgccc gggagtacgc ccagcgtctg
     2281 aagcagctgg ccgacgaagt gtcctccgaa ctcttcgtcg tgatgcgcac ctacttcgag
     2341 aagccgcgca ccaccgtcgg ctggaaggga ttgatcaacg acccgtacct ggacgactcc
     2401 ttcagagtcg gcgagggcct ggagattgcg cgccggctgc tgctggacat caccagcctg
     2461 ggcctgccgg tgagcaccga agcgctcgac cccatcaccc cgcagtacct ccaggacctg
     2521 atcacctggt cggccatcgg tgcccgcacc accgagtcgc agactcaccg ggagatggcg
     2581 tcggggttga gctgcccgat cggtttcaag aacggcacag acggctccct ggacgtcgcg
     2641 atcaacgctc tggaatccgc cgtgagcccg caccgttttc tggggatcag ctcatcggga
     2701 caggtctcgg tgatccgcac taccggcaac cgtcatgccc acatcgttct gcgcggcggg
     2761 agccgggggc ccaactacga cgccgagaac gtcgcggcct gtgaggcggc cctggagaag
     2821 atcggccagg ttcccaacat catggtcgac tgcagccatg ccaactccaa caaggaccac
     2881 cgccgccagc ctgacgtggc gcgaggcgta gccgagcaga tcgagaacgg gagccgctcc
     2941 ctgatggggc tgatgatcga gagcaacctt cacgagggca accagcccgt caacggcggg
     3001 agcaacctcc gctatggcgt atcggtgacc gatgcctgca tcgattggcc gacaacggag
     3061 tcgttgctgc gggagcttgc cgagcggctg ggccccgcac tgcgggcga
//
LOCUS       DBNHOX010000202         4674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_851731, whole genome shotgun sequence.
ACCESSION   DBNHOX010000202 DBNHOX010000000
VERSION     DBNHOX010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_851731"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="AC16UZ_04455"
     CDS             complement(<1..569)
                     /locus_tag="AC16UZ_04455"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: protein motif:HMM:NF016382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003727 - single-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HIR6245964.1"
                     /translation="MPRHDRPNSVPRAPHRWRLAPRAAVAVQNRLRDRVRIVPGPKPR
                     RIVGLDCAFAENDVLAVAVVWDVVGGRVLETRGARRRVDFPYVPGLLSFREVPVMLDV
                     LRRLTTSFDAVMCDGQGIAHPRRLGLASHLGVILQMPTLGCAKSRLLGSHAPVGRRRG
                     DWVPLLDGDERVGTVLRTRDDVRPVFVSPG"
     gene            674..3169
                     /locus_tag="AC16UZ_04460"
     CDS             674..3169
                     /locus_tag="AC16UZ_04460"
                     /inference="COORDINATES: protein motif:HMM:NF025761.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HIR6245965.1"
                     /translation="MKQEPLTEPYQRRQLADLPPSGVAYDEVLDGRELRHHWQFLDGT
                     FTATDSRSPEALNRTVQQILDESAVTYLEDGAQRPWRLDGLPYVLDPNEWPTLTRGLE
                     QRARLLNAVVADLYGPQTLLHRGLPLGLAFANPDYLLPACGYSAPEAVFLHLLAFDLG
                     RSPDGQWRVLSSRSEAPSGLGYTLENRLIMARSVPELFESGGIARIAQFFRAHSEYLH
                     ELGETTGRSDGLSVILSGGPGQESYFEHTFLGRYLGYPVVEGADLTVRDGEVFLKTLE
                     GLKPVSLIVRQVESSDCDPLELRAHSLQGVPGLLRAASTGRVAVANAIGSGLVENEAF
                     ASFLPSLCKQMLSEPLELPDLTTWWCGQPAELEHVLANRGELVLRNAFHRKPLLASSV
                     GAYMASDILPTGNASIPAKISRLPYQFVGRERIELSTVPYWTGADGWARAPMTLRLYV
                     GATGDGYKVLPGGLARLATPEGDISKDVWIPAAGAEWPATPPARTVATRRSDRDLSSR
                     TGDDLFWLGRYLERTEGAVRLYRALFSYAGGEGAASGPPVELGQLTRLLTTMDYLSPA
                     RARRAAAAGRGAFDQELWQILFDPESEDGLANVLGNVRRTAEHVRERLSRDAWRLFEG
                     LSAIPELRWRVHSIADALRLLDQLIEKLSAVNGQVHENMTRGYGWRLLDMGRRLERCT
                     YIVRVVKDLCTREPQQIGALNLLLDVCDSGLTHRFRYQTSPTLATVLDLLLVDDTNPR
                     SVAYQIGMLREHMATMPLEQRDGSLSESGRILLAAQSELALADTVKLAEVISRKGLRT
                     HLNRLLGRLEESMNGLHEVVTRTYFDHTISHWQ"
     gene            3171..4070
                     /locus_tag="AC16UZ_04465"
     CDS             3171..4070
                     /locus_tag="AC16UZ_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HIR6245966.1"
                     /translation="MTRYQIVHKTRYEYARPVSSSYQHLHLTPRSFAKQNVISSDLHI
                     EPAPTSNQTRQDYFGNPVTDVVIRSRHDSLEITSRALLDVNADENILLDLSPPWEAVA
                     GAVGDPRSSEAWDAARFCFPSPHVDPREALAYAEPSLSPGTPFLRSVEHITARIYEEF
                     TYKGGVTDVQTPVEEVLQSRAGVCQDFAHLAIASVRAFGLPARYVSGYLLTRPPQGQP
                     RLTGADASHAWISVWCPEFGWIDFDPTNNLRPRDEHITLGWGRDYSDVSPTRGFIHGG
                     GSQQLEVSVDVTPVPLADHRARR"
     gene            4131..>4674
                     /locus_tag="AC16UZ_04470"
     CDS             4131..>4674
                     /locus_tag="AC16UZ_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR6245967.1"
                     /translation="MKDGTLLFSGIPYAAAPEGSLRFRPAAPVTPWTGVRPALKFGPA
                     APQVATGGLTNSAAVRWSEDCLSLNVCTPALDGALRPVLVWIHGGSFRTGQGAVPWYN
                     GNRFALHGDMVVVSINYRLGALGFSELSRLGSGFETSGVNGLLDQIRALEWVRDNIQQ
                     FGGDPDRVTIAGESAGAFSVC"
BASE COUNT          835 a         1594 c         1475 g          770 t
ORIGIN      
        1 ccaggactga caaacacggg ccgcacgtcg tcgcgcgtgc gaaggaccgt tcccacacgc
       61 tcatcgccat ccagcagcgg cacccaatcg ccgcggcgcc ggccaacggg agcgtggcta
      121 cccaacagcc gggatttagc gcagccaagc gtcggcattt ggagaatgac gcccaggtgc
      181 gatgcaagtc ccaggcgccg aggatgagca atgccctgac cgtcgcacat cacggcgtcg
      241 aaggaagtcg tcaggcggcg cagcacgtcg agcatcaccg gaacttcgcg aaaggaaagg
      301 agccccggaa cgtaaggaaa gtcgacccgg cggcgcgcgc cacgcgtctc caagacgcgc
      361 cctccgacga catcccagac caccgctacc gcgagaacgt cgttttccgc gaaagcgcaa
      421 tccagcccga cgattctccg cggtttcggt cccgggacga ttcggacccg atcccgcaga
      481 cgattctgca cggccaccgc ggcgcgaggc gcgagacgcc atctgtgcgg ggcccgagga
      541 acggaattgg ggcgatcgtg ccgaggcatt acggcttgca ggggccaagc cccccaccta
      601 accttgtccg gccccccact atgcacggcg ggatccggtt cgcaaagccg acctttctga
      661 gcgacaggcc aacttgaagc aggaacctct cacagaacct taccagagaa ggcaactggc
      721 cgacctgcca ccctcgggcg ttgcctacga cgaggtgctg gatggcaggg agctgcgcca
      781 tcactggcaa tttctcgacg gcacgttcac ggcgacggac agccgctcgc cggaagccct
      841 caaccggacg gttcagcaga tcctcgacga aagcgcggtg acctacctgg aagacggcgc
      901 tcagcgcccc tggcgtctcg acggcctgcc atacgtcctg gatcccaacg agtggcccac
      961 gctaacccgc ggcctggaac agcgggcgcg tctgcttaac gccgtcgtcg ccgatctgta
     1021 cggtccgcag acactgctgc accgcggcct gcccctcggt cttgcttttg ccaaccccga
     1081 ctacctgctg ccggcgtgcg gctactccgc ccccgaggcc gtctttctgc atctgctcgc
     1141 attcgatctg ggacgatcac ccgatgggca gtggcgggtg ctgagcagcc gcagcgaagc
     1201 cccttccggt ctgggctaca cgctggagaa tcggctgatc atggcgcgat cggtgcccga
     1261 gctgttcgag tcgggcggca tcgcgcgaat cgcgcagttc ttccgcgccc acagcgagta
     1321 cctgcacgag ctgggagaga cgaccggacg cagcgacggt ctttccgtca tcctctccgg
     1381 aggtcccggt caggaaagct acttcgagca caccttcctc ggccgctacc ttggctatcc
     1441 cgtggtggag ggagccgatc tgaccgtccg cgacggcgag gtcttcctga aaactctcga
     1501 agggctgaag ccggtcagcc tgatcgtccg gcaggtggag tcgtcagact gcgacccgct
     1561 ggagctgcgc gcgcattccc tgcagggggt gccaggcctg cttcgtgctg cgagcacggg
     1621 gcgggtggcg gtggcaaacg ccatcggcag cggcctggtg gaaaacgaag cgttcgcgag
     1681 tttcctgccc tcgctgtgca agcagatgct gagcgagccc ctcgagctgc cggatctcac
     1741 cacctggtgg tgcggccagc cggccgagct ggaacacgtc cttgccaacc ggggggagct
     1801 ggtgctgcgt aatgctttcc accgcaagcc gctgctggcc agcagcgtgg gcgcatatat
     1861 ggccagcgac attctcccga ctggcaacgc atcgattccg gcaaagatct cgcgcctgcc
     1921 ctaccagttc gtcgggcgtg agcgcatcga gctgtccacc gttccctact ggacaggggc
     1981 ggacgggtgg gcacgggcac cgatgacgct gcgtctgtac gtaggcgcca ccggtgacgg
     2041 ctacaaagtg ttgcccggcg ggcttgcacg cctggccaca cccgaaggag acatcagcaa
     2101 ggacgtctgg attcccgccg cgggggccga gtggccggcg acgccaccgg cgcgcacggt
     2161 ggcaacccgc cgcagcgacc gcgatctatc cagccgcacc ggtgacgacc tcttctggct
     2221 gggacgctac ctggagcgaa ccgagggcgc cgtgcgcctt taccgggcgc tgttcagcta
     2281 tgccggtggc gagggcgctg caagtggacc gcccgtcgag cttggccagc tgacaaggct
     2341 gctcacgacg atggactacc tctcaccggc cagagcgaga cgagcggccg cagccggccg
     2401 gggcgccttc gaccaggaac tgtggcagat cctctttgac cccgagagcg aggacggcct
     2461 ggccaacgtt ctcggcaacg ttcggcgaac tgcggagcac gttcgcgaac gtctttcgcg
     2521 cgacgcgtgg cgtctgttcg aagggctttc ggcgataccg gaactgcgct ggcgcgttca
     2581 ctccatcgcc gatgcgctcc ggctgctgga tcaactgatc gagaagctgt cggcggtcaa
     2641 tggccaggtc cacgaaaaca tgacccgcgg ctacggctgg cggctactgg acatgggtcg
     2701 acggctggag cgctgtacct acatcgtccg ggtcgtcaaa gatctctgta cccgggaacc
     2761 gcagcagatc ggcgcactga acctgctgct cgacgtgtgc gacagcgggc tcacccaccg
     2821 cttccgatac cagacgagcc ctacccttgc gaccgtcctc gatctgctgc tcgtcgacga
     2881 caccaaccca cgctccgtgg cttaccagat cggcatgctg cgcgagcaca tggcgaccat
     2941 gccgctggag cagcgggacg ggtcgctgag cgagtcaggc cgcattctgc tggccgccca
     3001 aagcgagctc gctctggcag acaccgtcaa gctcgcggag gtgatcagca ggaaggggct
     3061 gcgtactcat ctcaatcgtc tgctcggccg gctggaggaa tccatgaacg gtctccacga
     3121 agtggtgacc cgcacgtact tcgatcacac catcagccac tggcagtgag atgacgcgtt
     3181 atcagatagt tcacaagacg cgctacgagt acgctcgtcc ggtgagctcg tcctaccagc
     3241 atctgcacct cacgccgcgc agctttgcca agcagaacgt gatcagctcg gacctgcaca
     3301 tagagccggc ccccacttcg aaccagacgc ggcaggatta cttcggcaac ccggtcaccg
     3361 acgtcgtcat ccgcagcaga cacgactcgc tcgagatcac cagtcgtgcg ctgcttgacg
     3421 tgaatgcaga tgagaacatc cttctggatc ttagcccgcc gtgggaagcc gtcgccgggg
     3481 cggtcggcga tcccagatcg agcgaggcct gggatgccgc ccgcttctgc tttccctcgc
     3541 ctcatgtcga tccgcgggaa gctcttgcct acgccgagcc cagcctgtcg cccggcacgc
     3601 catttctgcg atccgtcgag cacatcacgg ctcggatcta cgaggagttc acctacaagg
     3661 gcggcgtcac cgacgtccag acgccggtgg aagaggtgct gcagtcgagg gccggggtgt
     3721 gccaggactt tgctcacctg gccatcgcca gcgtgcgggc gttcgggctg ccggccaggt
     3781 acgtcagcgg ctacctgctt acccgacctc cccaaggaca gcctcggctc accggcgcgg
     3841 atgcgtccca cgcctggata tcggtgtggt gcccggagtt cggctggatc gacttcgatc
     3901 cgaccaacaa tctccgccca agggacgagc acatcacgct gggctggggc cgcgactaca
     3961 gcgacgtcag ccccacccgg ggattcattc acggcggtgg aagccagcag ctggaagttt
     4021 ccgtggacgt gacacccgtc ccgctggccg atcaccgcgc ccgccggtaa cctcttccca
     4081 tgagccaacc aaacattgcc cagacccgaa ccggtgcagt agaaggccgc atgaaagacg
     4141 gcacgctcct gttttccgga ataccctacg ccgctgcacc ggagggttcc cttcgtttca
     4201 ggcctgcggc accggtaacg ccctggaccg gagtccgccc ggcgttgaag ttcggacccg
     4261 ccgctcctca ggtggcgacc ggcggtctca ccaacagcgc cgctgttcgc tggtccgagg
     4321 attgcctatc cctcaacgtc tgcaccccgg cgctggacgg agcgctgcgc ccggtgctcg
     4381 tgtggattca cggcggcagc ttccgcaccg gtcagggagc ggtgccctgg tacaacggca
     4441 accggttcgc gcttcacggc gacatggtcg tcgtcagcat caactaccgg ctgggcgcgc
     4501 tgggattctc cgagctgtcc cgcctcggat cgggtttcga gacctcaggg gtgaacggcc
     4561 tcctcgacca gatccgagct ctcgaatggg tacgggacaa catccagcaa ttcggcggcg
     4621 accctgaccg ggtcacgata gccggtgagt ctgcgggcgc tttcagcgtc tgct
//
LOCUS       DBNHOX010000203         2993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_916822, whole genome shotgun sequence.
ACCESSION   DBNHOX010000203 DBNHOX010000000
VERSION     DBNHOX010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_916822"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..396)
                     /locus_tag="AC16UZ_04475"
     CDS             complement(121..396)
                     /locus_tag="AC16UZ_04475"
                     /inference="COORDINATES: protein motif:HMM:NF016280.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HIR6245968.1"
                     /translation="MAEHSARNHSPGNEDVVGTFIERLTEQLASLVPPDSLRLVLDQA
                     RSALQRGLAEVDLVPRHELDAHLAALDRLTETTRDLERRVAALEADS"
     gene            770..1519
                     /locus_tag="AC16UZ_04480"
     CDS             770..1519
                     /locus_tag="AC16UZ_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HIR6245969.1"
                     /translation="MKKIITPVVAGILLFAAGASAPAAAEGPEFSGNIAYLTDYRFRG
                     ISQGDRSQALQGGFDIELDNGLYAGTWASNVGFTECCGSAGLELNYYAGFAGAFSEEV
                     DYDVGFLWYNYPEDDADPDLDYYELYGSVSAFGGTFGAAFSPDYFAETGDFWYLYGDY
                     SFGVAENLSIDLHVGFNLFEDEESFAAFIGPEDGDAGDNYIDYSIGVSTSALGLDFGL
                     SFIGTDLKKSECFGGTKLCDDTVVLSVSKSL"
     gene            1648..1986
                     /gene="glnK"
                     /locus_tag="AC16UZ_04485"
     CDS             1648..1986
                     /gene="glnK"
                     /locus_tag="AC16UZ_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR6245970.1"
                     /translation="MKLVTAIIKPFKLDDVREALSDIGVQGMTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEIAIDDGLLEQCLETITKSANTGKVGDGKIFVSSLEDVVRI
                     RTGETGAEAV"
     gene            2085..>2993
                     /locus_tag="AC16UZ_04490"
     CDS             2085..>2993
                     /locus_tag="AC16UZ_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter family protein"
                     /protein_id="HIR6245971.1"
                     /translation="MGALVMFMAPGFAMLEAGLVRSKNTAEILLKNVALFGIACIMYL
                     ICGYYIMYVGAVEGGILPDFGLWIGAENTVDETLASGGDVYYSARSDYFFQVVFVATA
                     MSIVSGAVAERMNLWAFLAFAVVMTGLIYPLQGMWTWGGGWISTAGYSDFAGSGIVHM
                     CGAAAALAGVLLLGPRKGKYNKDGSVNALPGANLPLATLGMFILWFGWFGFNGGSELK
                     TSNIDEANAVAQVFVNTNIAACGGLVAALLTAKMLFGKADLTMALNGALAGLVAITAD
                     PLSPIAQWAMIIGAVEGGILPDFGLWI"
BASE COUNT          553 a          889 c          962 g          589 t
ORIGIN      
        1 agatgtgtct ccaccgtaac cgcaggcgtg tccatgccgg cctgggcgcg ggtacgggtc
       61 aggcttttct cggtggccga cttgagcgta tggccgtccg gatcgttcac cccgatcgga
      121 tcaggaatct gcttccaggg cagcgacccg gcgctctaag tcacgggtgg tttcggtcag
      181 cctgtccaga gcggccaggt gggcgtcgag ctcgtgccgg ggaacgagat ccacctcggc
      241 aaggccgcgc tgcagcgcgg agcgggcctg gtccaggacc aggcgcaggc tgtcgggcgg
      301 cacgagggat gccagctgct cggtgaggcg ctcgatgaac gttcccacca cgtcctcgtt
      361 gccgggtgag tggtttctgg ctgagtgctc ggccattgcg cgtccgccca ttggtttgga
      421 tgacgacgat ggtactcaca gctgccgtcg ccggtcgact ggggaatcct cgcccaggcg
      481 taagccaccg acccgggccg gtgcaggccg gctctgaact ggcgcgctac tttggtgcag
      541 atgccttatt tcggtgcaac agcccggctc ggaagccggt tatctcctgc cggtccagcc
      601 aagcattcct tcaaccagct gatttttatg aagaaaaatc tggtggcacg gaaaatgctc
      661 ctttgagcgc gtcccgtcag ttctgccgcc ctgcggtggg cagaaaaggg gggagcgatc
      721 acccggtcgc gacacaaggt ttttagagca agagcacaga ggtttactca tgaaaaagat
      781 cattaccccg gttgtggcgg gaatcctcct cttcgcagcg ggtgccagcg cgccagccgc
      841 cgccgaagga ccggagttct ccggcaacat cgcatacctg accgactacc gcttccgcgg
      901 tatctcccag ggagatcggt cccaggccct gcagggcggt ttcgacatcg agctggacaa
      961 cggcctctac gcgggaacct gggcgtccaa cgtcggcttc acggaatgct gcggctccgc
     1021 gggactcgag ctgaactact atgctggttt tgccggtgcg ttcagcgagg aagtcgatta
     1081 cgacgtcggg ttcctctggt acaactatcc cgaggacgac gcggacccgg acctcgacta
     1141 ctacgagctt tacggcagcg tcagtgcctt cggcggcacg ttcggggctg ccttctctcc
     1201 cgactacttc gccgagaccg gcgacttctg gtacctctac ggcgactaca gcttcggggt
     1261 cgccgagaac ttgagcatcg atctgcacgt gggcttcaac ctcttcgagg acgaggaatc
     1321 cttcgcagcg ttcatcgggc ctgaagacgg cgatgccgga gacaactaca tcgactacag
     1381 catcggcgtg tccacgtccg cgctgggtct cgacttcggc ctctctttca tcggcacgga
     1441 cctcaagaag agcgagtgct tcggcggcac caagctgtgc gacgacaccg tcgtgctctc
     1501 ggtatctaaa agcctgtaat tgagagagaa tccggggtgc acagatcttc cacaaagatc
     1561 ttcccagagc agtccgcgtc agcgggctgc gctcggggag agcgtgcacc ttagaaaccg
     1621 aaaacgggtt caacgggaga acaaaacatg aaactggtaa cagccatcat caagcccttc
     1681 aaactcgatg atgtacggga ggcgctctcg gatatcggag tgcaggggat gacggtcact
     1741 gaagtgaaag ggttcggtcg ccagaaaggg cacaccgaac tctatcgggg ggcggagtac
     1801 gtggtcgact tcctgcccaa ggtgaagatc gagatcgcca tcgacgacgg gctcttagag
     1861 caatgcctgg agaccatcac caaatcggcc aacaccggca aggtgggcga cggcaagata
     1921 ttcgtttcct cgctcgaaga cgtcgtgcgg atccgcacgg gagagacggg ggccgaggcg
     1981 gtctgacgcc atgcgctagt cgcctttaaa aaaattcgga gaagattgtg gaagatctgt
     2041 tgactcaaac taattacgcc ctggacacct tttattttct ggtgatgggt gcgctcgtca
     2101 tgttcatggc gcccggtttc gcgatgctcg aggccggact ggtacggtcc aagaacacgg
     2161 cggaaatcct tctgaagaac gtcgccctgt tcggcatcgc gtgcatcatg tacctgatct
     2221 gcgggtacta catcatgtac gtcggcgccg tcgagggggg gatcctgccc gacttcggcc
     2281 tgtggatcgg tgcggagaac acggtggatg aaaccctggc ctcgggaggc gacgtctact
     2341 actcggcgcg ctccgactac ttcttccagg tggttttcgt ggctaccgca atgtccatcg
     2401 tctcgggggc agtcgccgag cgcatgaatc tatgggcctt cctggccttt gcggtggtca
     2461 tgacgggcct gatttacccg ctgcagggca tgtggacctg gggcggcggc tggatctcca
     2521 cggccggcta ctcggacttc gccgggtccg gcatcgtgca catgtgcggc gcggcggcag
     2581 cgctggccgg ggtcttgctg ctcgggccaa gaaagggcaa gtacaacaag gacgggtcgg
     2641 tgaacgcgct gcccggcgcc aacctgcccc tggcgaccct cgggatgttc atcctctggt
     2701 tcggctggtt cggattcaac ggcggctcgg agctcaagac gtcgaacatc gacgaggcga
     2761 acgcggtggc ccaggtgttc gtcaacacca acatagctgc ctgcggcggc ttggttgccg
     2821 cgctgctgac cgccaagatg ctcttcggga aggccgatct gaccatggct ctgaacgggg
     2881 cgctggccgg tctcgtggcc atcacggcgg atccgctctc gcccatcgct cagtgggcga
     2941 tgatcatcgg cgccgtcgag ggggggatcc tgcccgactt cggcctgtgg atc
//
LOCUS       DBNHOX010000204         8249 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_467473, whole genome shotgun sequence.
ACCESSION   DBNHOX010000204 DBNHOX010000000
VERSION     DBNHOX010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8249)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_467473"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1532)
                     /locus_tag="AC16UZ_04495"
     CDS             complement(72..1532)
                     /locus_tag="AC16UZ_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6245972.1"
                     /translation="MTKGYQVRLPTKIVYGLGSVSDGVKNTTFNVFLLFFYTQVAGLS
                     GSLAGAAILIALVFDAVSDPLIGNISDNFHSRWGRRHPFMYAAALPMAVFFGLIFSPP
                     QGLGQAGLFAWMLTMAIGVRVSMTLYAIPSGALVAEMTSHYDERTSLVSFRVLSGWLG
                     GLVASQMGYLVFFAPSAAYADGRLDPQAYGDYAVFGAITIFCAILICAIGTHRLIPEL
                     KSPPAHTPFTLRRFGVELREVFSNRPYLVLVFTVLVVSTATGFTDVMGLYVNTYFWEF
                     TTAQLAIIVFGALAGVVAAFSVTATLAQRTDKRTMALAMLLVVVIIGPLPVVLRLLGL
                     MPANGDPLLLGIMIAWTGVIVFVAVGVTILAGSMIADTVDLNELRTGKRQEGMFSAAM
                     GLTAKATSGIGGFVGGVVLDVVDFPTGVAAGEVDADTLFALGVAVGPGMFLLWITVLI
                     ILSRYRLSRADHADILGKLEARRNAAEQPGGEAAGG"
     gene            1782..2393
                     /locus_tag="AC16UZ_04500"
     CDS             1782..2393
                     /locus_tag="AC16UZ_04500"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6245973.1"
                     /translation="MKFYNSIGPNPQVVRTFAAERGIELDMEEVDLMAADNRREPYLS
                     KNPAGQSPCLELDNGDYVSEVTAICEYLDETSPGTSLIGNTPEERAETRMWVRRIDLN
                     ICEPLGYGFRFGEGLDFFKDRITTLPEASEGMKRIAAEKIAWLDGLMAGREFVCGDRI
                     TLADVLLYCFLAFGKTVGQPYAETNQNIHAWFERMSKRPSVSA"
     gene            2411..3415
                     /locus_tag="AC16UZ_04505"
     CDS             2411..3415
                     /locus_tag="AC16UZ_04505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6245974.1"
                     /translation="MRFSLMLPVFVDAGAASPFERTLGFARTADELGYHMGTFGHHSF
                     TPQIADPSAPFALLSAVAARTERLRLGTGVYLAPLHHPVTVAEQAAMLDQISGGRAVL
                     GVGLGYRPYEYEGFGLDFHTRGKRLDELLTAIREGWTRGEFRHDGEHFRIPPSPVNPP
                     CVQKPQVPSLVGGSAPAAITRAARLGDGWFALPQEGLEIMRRQVDAYRAACREAGRTP
                     YVCLMRNAWIAPTMEQVQAEWLPRMVEFHKTFADARADRSDTVIERLLAGETFTLDEY
                     IRARAIAGTPEVCIAEIERWQAAIQPDEYSLIFGGSDDAARLEDAVTLFAREVMPAFS
                     "
     gene            3430..3849
                     /locus_tag="AC16UZ_04510"
     CDS             3430..3849
                     /locus_tag="AC16UZ_04510"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6245975.1"
                     /translation="MAASLEDNPMPVNLIDYMPFARVLGLEVTHSDKDKVTASIEVSE
                     DLCTTGKIMHGGAVMAVADTLGAIGAYLNLPDGAKATTTIESKTNFLGAARLGTRVLA
                     ETTPVHVGGRTSVWQTRLTGEDGKPVALVTQTQLVLS"
     gene            3941..5863
                     /locus_tag="AC16UZ_04515"
     CDS             3941..5863
                     /locus_tag="AC16UZ_04515"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIR6245976.1"
                     /translation="MADIFISYARSTRRQAEAIGEALVELGYSIWRDDALPAHRAYAD
                     EIQKQLNIAKAVVVVWSADAIKSDWVRSEADRARNDRKLVQLTVDGAALPMPFDQIQC
                     ANLAGWRGEGRAAGWATVLESIRALTDTAGKESPVSAGTQSQQQPEPILAVLPFDNLS
                     TDPEMQFFSDGVSEEIIQRLSRGASLKVIGRTSSFQFRGERKAEAASALGCSHILDGS
                     VRRGAGRMRLSAHLVETSSGTTLWSERYDRSLEDVFAVQDDVSENIAGALHHTFQSFS
                     TPAVDPGAYDLYLRASPESYAPEEMRTNIGLLEVATQRAPDFPEAWGRLAYLRAFLRF
                     YEPFPGRGASAAIIARESAHALQADPENGDALAARAFVVAPFGNFIELDTAVERLRSA
                     PGDFDARKFAGWLLRNFGRVRESLAEDERAYLGNALDPMCANTLALARLAAGRVAEAL
                     PVLENLVERVPDMVFPFANLLRAQALLENWEALDELLELNADRPLREFQDTLDFIRTK
                     RNPSAENIAVWKSQFEAHIARTGCIDISRVVYAAHLGLVDEAYQATEQAHLGPTGTNE
                     DVMGPDGYRTSLLFQAAMPELRNDPRFVPLCARLGLVEFWTSTGKWPDCADEVPYDFR
                     AACEDARGIPKLEFGF"
     gene            complement(5908..7248)
                     /locus_tag="AC16UZ_04520"
     CDS             complement(5908..7248)
                     /locus_tag="AC16UZ_04520"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6245977.1"
                     /translation="MVTSRQEALVQRSQAVSGSVSRIFPADVTITGERYTSREFMELE
                     LDRLWPRVWNIGAWAREIPVAGDFVTHAIGRESILMVRQADGSVRAFHNVCPHRGNRL
                     VYSDRGSVERFTCSYHGWQFDQAGTVVHVQDEEDFPRGNPCGKLTLAEIPCEVWAGFV
                     WYNMDAECASLAEFLGEVKDYVDLHDLSAARRIYYKVCEVGFNWKLLHDNFCESYHLP
                     ATHPQISDYYDDDYRNTDFELYETGHNLMKMKGSLPSLRYDEPFAINETLAADMQNWG
                     LDPAAFEGRAHAVREALQAQRRKLGPARGYRYFERLPASWLTDAYHFNLFPGTSLTVV
                     ADGAQMQRCEPHPTDPTRSIYEHWFLALKGADSDLVPGPEGMVPWEEAERRVVEYGQE
                     TLSEVADQDLERASGQQLGVQSRGFAGAYLSGQENRVQQFHNFIDRYLRDDIGS"
     gene            7451..>8249
                     /locus_tag="AC16UZ_04525"
     CDS             7451..>8249
                     /locus_tag="AC16UZ_04525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019821071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6245978.1"
                     /translation="MEPSEATDTLIRLACFIGALVLFALLEAAFPRRQRQVSRKARWP
                     SNLGISALNQLVLRLVLPASAIALAVRLEQEGWGLLAQLTWPTWIELAAAVLILDLAI
                     YLQHRLYHAVPFLWRFHRMHHADLDFDVTTGIRFHPLSLLLSGLIKLGAVLIIGPSPF
                     AVLVFEVLLNATTMFNHSNLKLPRPFDRILRLVIVTPDMHRVHHSADPAETNRNFGFN
                     FPWWDRLFKTYRPQPALGHEAMTVGLEDFRSAGELRLDRMLSQPFRSG"
BASE COUNT         1437 a         2807 c         2642 g         1363 t
ORIGIN      
        1 cggccttcct ttgatctgag tgaccgccga gtgtgccacc ggcgctgggc ggaatgcaaa
       61 gcagcgctcg gctagccccc ggcggcttcc ccgccgggct gctcggcggc gttccggcgg
      121 gcttcgagct tgcccaggat gtccgcgtga tcggcccggc tcagccgata gcgcgacagg
      181 atgatcaaca ccgtgatcca gagcagaaac atgccggggc ccacggccac gcccagcgcg
      241 aagagggtat ccgcatccac ctcgcccgcg gccacgcccg tcggaaagtc gaccacgtcc
      301 aggaccacac cgccaacgaa gccgccgatg cccgatgtcg ccttggccgt caggcccatg
      361 gccgcgctga acatgccttc ctggcgtttg cccgtgcgca gctcgttgag gtccacggtg
      421 tcggcgatca tggagccggc cagaatcgtc acgcccaccg cgacgaacac gatcacgccc
      481 gtccaggcga tcatgatgcc gagcagcagc ggatcgccgt tcgccggcat gaggcccagc
      541 agccgcagca ccacgggcag aggtccaatg atcaccacca ccagcagcat cgccagcgcc
      601 atggtgcgct tgtccgttcg ctgcgcaagc gttgccgtga ccgagaaggc cgctaccacg
      661 ccggccagcg cgccgaagac gatgatggca agctgcgcgg tggtgaactc ccagaagtac
      721 gtgttcacgt acagacccat gacgtcggtg aagccggtgg ccgtggagac gacgagcacc
      781 gtgaacacga gcacgagata cgggcggttg ctgaacacct cgcgcagctc gacgccgaac
      841 cggcgcaggg taaacggtgt atgcgcgggc ggcgatttca gttcgggaat caatcgatgg
      901 gtgccgatcg cgcagatcag gatggcgcag aagatcgtga tggcgccgaa gacggcgtag
      961 tcgccgtaag cctgaggatc caggcgaccg tccgcgtagg cggcagacgg ggcaaagaag
     1021 acgaggtagc ccatctggga ggcgaccagc cctcccagcc agccggagag cacccggaag
     1081 ctgaccaggg acgttcgctc gtcgtagtgg ctcgtcatct ccgccaccag cgcgccgctg
     1141 ggaatggcgt acaacgtcat ggacacccgg acaccaatgg ccatggtcag catccaggcg
     1201 aagagtccgg cctggcccag cccttgcggc gggctgaaga tcagcccgaa aaagacggcc
     1261 atgggcagcg cggcggcata catgaacgga tggcggcggc cccagcggga gtgaaagttg
     1321 tccgagatgt tgccgatgag cgggtcgctg accgcatcga acaccagcgc gatgaggata
     1381 gccgcaccgg ccaacgagcc ggagaggccc gcaacctggg tgtagaagaa gagcagaaaa
     1441 acgttgaacg tggtgttctt gacgccgtcg gaaacgctgc caagcccgta gacgatcttg
     1501 gtgggaagcc tgacctgata gcctttcgtc acgggacctt ccacgatgcc tcaatatcac
     1561 ggccccgccg ggggcggacc cgggcacacg gctatcggca gaatccgggc tccggggaag
     1621 gtcggcgagt ctacgcgcct ggaacggtgc ttgcacctgc ccgcgcggcc cgtacaaccc
     1681 gggcatcagc ctccaggccg gcggcgcgcc ggtcgcccgt ggtggtgcgc agcggcgccg
     1741 gcccctagaa tccgacgttt gaatcaatcg ggtaacacgc aatgaaattc tacaactcca
     1801 tcggcccgaa ccctcaggtg gtgcgcacgt ttgccgccga gcgcggcatc gaactcgaca
     1861 tggaggaggt cgacctcatg gcggccgata accgccggga gccctacctg tcgaagaacc
     1921 cggccggcca gtcgccgtgc ctcgagctcg acaacggcga ctacgtcagc gaggtcacgg
     1981 caatctgcga gtatctcgac gagacgagcc caggcacgtc tctgatcggc aacacgccgg
     2041 aggagcgcgc cgaaacgcgc atgtgggtgc gccgcatcga cctgaacatc tgcgagcccc
     2101 tgggctacgg cttccgcttc ggcgagggat tggatttctt caaggaccgc atcaccaccc
     2161 taccggaagc ttccgaaggc atgaagcgca tcgcggcgga gaagatcgcc tggctcgacg
     2221 gcctcatggc cggccgcgag ttcgtctgcg gcgacaggat cacgctggcc gacgtgctgc
     2281 tgtactgctt cctcgctttc ggcaagaccg tgggccagcc ctacgcggag accaatcaga
     2341 acatccacgc gtggttcgag cggatgtcta agcggccaag cgtgtcggcg tgattcccga
     2401 gtaagcgccc atgaggttca gtctcatgct gccggtgttc gtggacgccg gggccgcgag
     2461 ccccttcgaa cgaacactgg gcttcgcccg caccgcggac gagctcggct accacatggg
     2521 caccttcggc catcactcgt tcacgccaca gatcgccgat ccgtccgccc cgtttgcgct
     2581 tctgtcagcg gtggcggcgc gtaccgagcg gctgcgcctg ggcaccggcg tctacctcgc
     2641 cccgctgcac catccggtga ccgttgcaga gcaggccgcc atgctggacc agatatccgg
     2701 cgggcgcgcg gtgctggggg tcggcctcgg ctaccggccc tacgagtacg agggcttcgg
     2761 gctcgatttc cacacccgcg gaaagcggct ggacgagctg ctcaccgcga tcagagaagg
     2821 atggacgcgg ggcgaatttc gccatgacgg cgagcacttc cgcataccgc cgtcgccggt
     2881 gaacccgccc tgcgtgcaga agccccaggt gccgagtctg gtgggtggct cggcacccgc
     2941 cgccatcacc cgggccgccc gtctgggaga cggctggttc gccctgcccc aggaaggcct
     3001 ggagatcatg cggcgccagg tggacgccta ccgcgcagcg tgtcgggaag ccgggcgcac
     3061 accctacgtc tgcctgatgc gcaacgcctg gatcgctccc accatggaac aggttcaggc
     3121 cgagtggctg ccgcgcatgg tcgagtttca caaaaccttc gccgatgccc gggccgatcg
     3181 gtccgacacc gtcatcgagc gcctgctggc cggggagacg ttcacgctgg acgagtacat
     3241 ccgagcgcgc gccatcgccg gcacgccgga ggtctgcatc gccgagatcg agcgctggca
     3301 ggcggccatc cagcccgacg agtattcgct catcttcggc ggctcggacg acgccgcgcg
     3361 gctcgaagac gcggtgaccc tgttcgcccg cgaggtcatg ccggcgttta gctgaacacg
     3421 gacgcccgga tggcggcgtc attagaggac aaccccatgc ccgtgaacct gatcgactac
     3481 atgcccttcg cccgcgtgct ggggctcgag gtgacccatt cggacaaaga caaggtgacc
     3541 gcatccatcg aggtgagcga ggatctgtgc accaccggca agatcatgca cggcggggcc
     3601 gtgatggcgg tggcggacac cctcggcgcc atcggcgcct acctgaacct gcccgacggc
     3661 gccaaggcca ccaccaccat cgagagcaag accaactttc taggtgcggc gcgtctgggc
     3721 acccgagtcc tggcggagac gacgcccgtc cacgtgggcg ggcgcacctc cgtgtggcag
     3781 acgcgcctca ccggcgagga cggcaagccc gtcgccctcg tcacccagac gcagttggtt
     3841 ttgagctgac cggctgacgg cggcagtact tgggtctgcc caaccggttt ctggtttccc
     3901 gttatactcg ccgtcgctcc tgtcaccagg tcgagcgtta gtggccgaca tcttcatctc
     3961 gtacgcgcgg tcgacccggc gccaggcaga ggccattggc gaggccctgg tagagctcgg
     4021 ttactccatc tggcgagacg acgccctgcc cgcccatcgc gcctacgccg acgagatcca
     4081 aaagcagctc aacattgcca aggcggtggt agtcgtctgg tccgcggatg cgatcaagtc
     4141 ggattgggtg cgctccgaag ccgaccgtgc gcgcaacgac cgcaagctcg tccagctgac
     4201 cgtcgacggg gcagcgctgc ccatgccgtt cgatcagatt cagtgcgcca acctcgccgg
     4261 ctggagggga gaaggccggg ccgccggctg ggcgacggtg ctggagagca ttcgtgcgct
     4321 caccgataca gccgggaaag aatcaccggt gtctgcgggc acgcaatcgc agcagcagcc
     4381 ggagcccatc ctcgccgttc tgcccttcga caacctctcc accgatccgg agatgcagtt
     4441 tttctccgac ggggtcagcg aggagatcat tcagcggctg tcccgggggg cgagcctcaa
     4501 ggtcatcggc cggacgtcga gctttcagtt tcgcggggag cgcaaggcgg aagccgcctc
     4561 tgcactcggc tgcagccaca tcctggacgg ttcagttcga cgcggcgcgg ggcggatgcg
     4621 cctgagcgcg cacctcgtgg agacgtcctc gggcaccacg ttgtggtcgg agcgctacga
     4681 ccgttccctg gaggacgtgt tcgccgtcca ggacgacgtt tccgagaaca tcgccggggc
     4741 gctgcatcac acgtttcaga gtttctcgac ccccgccgtg gacccaggcg cctacgacct
     4801 ttaccttcgg gcgagccccg agtcctacgc gcccgaggaa atgcgcacga acatcggcct
     4861 gcttgaagtt gccacccagc gtgcgcccga ttttcccgag gcctggggcc ggctcgccta
     4921 cctgcgagcc ttcctgcggt tctacgaacc gtttcccggg cgcggcgcca gcgccgcgat
     4981 catcgccaga gaatcggccc atgctttaca ggcggatccc gagaacggcg acgccctggc
     5041 cgcgagggca ttcgtggtcg cgcctttcgg aaacttcatc gagctcgaca ctgccgtcga
     5101 gcgcctgcgc agcgcgcccg gagacttcga cgcgagaaag ttcgccggtt ggctgctgag
     5161 gaacttcggc cgggttcgcg aaagcctcgc cgaggacgag cgcgcctacc tcggcaacgc
     5221 cctcgacccc atgtgcgcca atactctggc tctggcccga ctggcagcgg gacgcgtcgc
     5281 ggaagcgctg ccggtcctgg agaatctggt cgagcgcgtt cccgacatgg tttttccttt
     5341 cgccaacctg ttgcgtgccc aggccctgct cgagaactgg gaggcgctgg acgagctgct
     5401 cgagctcaat gccgaccgtc ccttgcggga atttcaggac actctggact tcatacgcac
     5461 caagcggaac ccgtcggcgg agaacatcgc cgtctggaag agccagttcg aggcgcacat
     5521 cgcgaggaca ggctgcatcg acatctcccg cgtcgtctac gctgcccatc tcggcctggt
     5581 cgacgaagcc taccaggcca ccgagcaggc ccacctggga cccaccggca cgaacgaaga
     5641 cgtcatgggc ccggacggct accgcacgtc gctgctgttt caggcggcca tgcccgagct
     5701 gcgcaacgac ccgcgcttcg tacccctgtg cgcccggctg gggctggtgg agttctggac
     5761 atccaccggc aagtggccgg attgcgccga cgaggtgccc tacgacttcc gcgccgcctg
     5821 cgaggatgcg cgcggcatcc ccaagctgga gttcggtttc tgagacgcag gcccggacgc
     5881 gcgggactgc cagcgcagcg ggtgccgcta ggaaccgata tcgtcgcgca gatagcggtc
     5941 gatgaagttg tggaactgct gcacccgatt ctcctgacct gagaggtagg cgcccgcaaa
     6001 tcccctcgac tgcactccga gctgctggcc tgaggcgcgc tcgagatcct gatcggccac
     6061 ctcgctcaac gtctcctggc cgtactccac cacccgccgc tcggcctcct cccagggcac
     6121 catcccttcc ggcccgggca ccaggtccga gtctgcgccc ttcagcgcca ggaaccagtg
     6181 ctcgtagatg ctccgtgtgg ggtccgtggg gtgcggctcg cagcgctgca tctgggcccc
     6241 gtccgccacc acggtgagcg aggtgccggg aaagaggttg aagtgatagg catccgtcag
     6301 ccaggacgcc ggcagacgct cgaagtagcg gtagcccctc gccggcccca gcttgcgcct
     6361 ctgcgcctgc agagcctccc gcacggcgtg cgcgcgcccc tcgaaggcgg cggggtcaag
     6421 cccccagttc tgcatgtcgg cggcgagggt ttcgttgatg gcgaacggct cgtcgtaacg
     6481 cagcgacggc agcgagcctt tcatcttcat caggttgtgg ccggtctcgt agagctcgaa
     6541 gtcagtgttc cggtagtcgt cgtcgtagta gtcgctgatc tgcgggtggg tggccggcag
     6601 atggtacgac tcgcagaagt tgtcgtgcag cagcttccag ttgaagccga cctcgcagac
     6661 cttgtagtag atgcgcctgg cagccgaaag atcgtgcagg tcgacgtagt cctttacctc
     6721 gccgagaaac tctgcgagcg aggcgcattc ggcgtccatg ttgtaccaga cgaacccggc
     6781 ccagacctcg caggggatct ctgccagcgt gagcttcccg cacgggttcc cccggggaaa
     6841 gtcctcctcg tcctgcacgt gaacgaccgt cccggcctgg tcgaactgcc agccgtgata
     6901 gctgcaggtg aagcgctcca cggatccccg gtccgagtag accagccggt tgccgcggtg
     6961 ggggcagacg ttgtggaagg cacggaccga gccgtccgcc tgtcgcacca tgagtatcga
     7021 ctcccggccg atcgcgtggg tgacgaagtc acccgccacc gggatctccc gcgcccaggc
     7081 cccgatgttc cagacccggg gccacaagcg gtcgagctcc agctccatga actcgcggga
     7141 ggtgtatcgc tcgccggtga tggtaacgtc agcgggaaag atccggctca cgctccccga
     7201 tacggcttgg ctgcgctgaa cgagcgcttc ctgtctggac gtaaccatgt cgctcccctt
     7261 ctccaacctg gcgcggccgc ttgcacgaca accctcttcc gcttgccgtg cgtcaacgcg
     7321 tccaacgatt attacagcca ccacagcgat ttatccagag cgcaaaagcg agggcggccg
     7381 tcggcgccgg ccgtcaggcg acggacgcct taaggggcac cagggggcca atgcgagcat
     7441 ccttacaccc atggaaccga gcgaagctac cgatacgctc atccgcctgg catgttttat
     7501 cggcgcgctg gttttgtttg ccctgctgga ggcagcgttt ccccggcggc agcgccaggt
     7561 gtcccgcaaa gcacgctggc ccagcaacct tggcatctcc gctctgaacc agctggtcct
     7621 gcggctggtg ctgcccgcct ctgcgatcgc gctcgccgtc cgcctggagc aggagggctg
     7681 gggcctcttg gctcagctga catggccgac ctggatcgag ctggcggcgg cggttctgat
     7741 cctggatctg gccatctacc tgcagcatcg gctctatcac gcggtccctt tcctatggcg
     7801 ctttcaccgc atgcaccatg ccgacctcga cttcgacgtc accacgggca tccggtttca
     7861 tccgctctca ctgttgctct ccggcctcat caagctcggc gcggttctga tcatcggacc
     7921 gtcacccttc gccgtgctcg tcttcgaggt gctgctgaac gcaaccacca tgttcaatca
     7981 cagcaacctg aagcttcccc gcccgttcga tcggattctg cgactcgtca tcgtgacccc
     8041 cgacatgcac cgcgtgcacc attccgctga tccggccgag acgaatcgga atttcggctt
     8101 caacttcccg tggtgggatc gcctctttaa aacctaccgg cctcagcccg cgctcggcca
     8161 cgaagccatg acggtgggcc tggaggattt cagatcagcg ggcgaactcc ggctcgaccg
     8221 catgctttct caacccttcc ggagcggta
//
LOCUS       DBNHOX010000205         1519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_274272, whole genome shotgun sequence.
ACCESSION   DBNHOX010000205 DBNHOX010000000
VERSION     DBNHOX010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_274272"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="AC16UZ_04530"
     CDS             complement(<1..301)
                     /locus_tag="AC16UZ_04530"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6245979.1"
                     /translation="MAERIYIRPVSRRDRSEFLHMTQRSQHVHEPWISPPLSDATFHS
                     YLKRTEQDDHEGLLVCLTDNDDIVGVININNIVRGSFLSASLGYYVSATQMGHGYM"
     gene            complement(294..518)
                     /locus_tag="AC16UZ_04535"
     CDS             complement(294..518)
                     /locus_tag="AC16UZ_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6245980.1"
                     /translation="MRYLSRFALLCMGFALTTAGLMLWHAVGFGFHGIWFFDNDYRLH
                     PLHLLIVGLAMIPPALWEIFVLESRRRSRG"
     gene            complement(515..1441)
                     /locus_tag="AC16UZ_04540"
     CDS             complement(515..1441)
                     /locus_tag="AC16UZ_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024919.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIR6245981.1"
                     /translation="MILTERFADPDTGKRYPLRCGGPIGWRSAGDKHTAIIQLPPIPA
                     GAILVPSLSTAPSPERFQFVLRAASRLWALHEVPCRAAPQPSADTAVTTHVDCFHVHE
                     DLPRAELTVTVTGPSSPARYLLTVTARQLELERAPAPTRSAICKAPPFLSQMALGTSV
                     GPRVCSPACITMLLARHGHAADLKSVSESCFDAVTNLYGIWPLAIRAAAEQGSLGAVE
                     LFDDWEEPLSVLDAGMPFAASVRFGEGELPGAPLTRTAGHLVVVHGADPQDVHVNDPR
                     APEAASVPRRYPAVDFSRAWLRHRGAAYILLP"
BASE COUNT          299 a          460 c          512 g          248 t
ORIGIN      
        1 gcatgtagcc gtgacccatt tgcgttgcgc tgacgtagta gcccagggaa gcgctcagaa
       61 acgaacctcg gacgatgttg ttgatgttga tcacccccac gatgtcatcg ttgtccgtca
      121 ggcagaccaa cagcccctcg tgatcgtcct gctcggtgcg cttgaggtag ctgtgaaacg
      181 ttgcgtcgct caggggtgga gatatccacg gctcgtgaac gtgctggctg cgctgcgtca
      241 tatgcagaaa ctcgctgcgg tcccggcgcg agaccggtcg gatgtagatg cgttcagcca
      301 cggcttcttc gtcggctctc cagcacgaag atctcccaca gcgcgggcgg gatcatggcg
      361 agaccaacga tgagcaggtg cagcggatgc aatcggtagt cgttgtcgaa aaaccagatg
      421 ccatgaaagc cgaagccaac ggcgtgccag agcatgagtc cagccgtcgt cagtgcgaag
      481 cccatgcaca gcagcgcgaa gcgcgaaaga tatctcatgg cagcaaaata tacgctgctc
      541 cccggtgcct aagccaggct ctggagaaat cgacggcagg gtagcggcgc ggtacggaag
      601 ccgcttccgg cgcccgcgga tcgttgacgt gaacgtcctg cggatcggcg ccgtgcacga
      661 ccacgagatg accggcggtg cgggtcagag gcgctccggg cagctcgccc tcgccgaagc
      721 ggacgctcgc tgcgaatggc attccagcat ccagcacgct cagcggctcc tcccagtcgt
      781 cgaacaactc caccgcacca agggatcctt gctccgcagc ggcgcggatg gccaggggcc
      841 agattccgta aaggttggtc accgcgtcga agcaggattc gctcacggac ttcagatctg
      901 cagcgtgtcc gtgccgcgcg aggagcatgg tgatgcaggc gggcgagcac acgcgtggcc
      961 cgacagacgt ccccagtgcc atctgagaga gaaagggagg tgccttgcag atcgccgatc
     1021 gcgttggagc aggcgcacgt tcgagctcga gctggcgcgc cgtgacggtc aagagatagc
     1081 gagccgggct cgaagggccg gtgacggtga ccgtcagctc ggcgcgcggc aggtcctcgt
     1141 gaacgtgaaa acagtccacg tgcgtcgtga cggcggtgtc ggccgacggc tgcggcgccg
     1201 cgcggcaggg aacctcgtgc agcgcccaca gccgggaagc agcgcgaagc acgaactgga
     1261 accgctccgg ggacggcgcc gtcgagaggc tgggtacgag aattgcgccg gccggtatgg
     1321 gcggcagctg gatgatcgcg gtatgtttgt caccggccga ccgccatccg atgggccccc
     1381 cgcagcgaag agggtaccgc ttgccggtgt cagggtcggc gaagcgttct gtcagtatca
     1441 tgagttccag cgattgacca acatgaacga aaccggcctg attctcttct ccacagacca
     1501 ctgcgcgcta tgcgacgag
//
LOCUS       DBNHOX010000206        10521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1688576, whole genome shotgun sequence.
ACCESSION   DBNHOX010000206 DBNHOX010000000
VERSION     DBNHOX010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10521
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1688576"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="AC16UZ_04545"
     CDS             <1..983
                     /locus_tag="AC16UZ_04545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014756520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR6245982.1"
                     /translation="GGSMVGVYRIHEAGGPEVLSWESEELPDPTGELVRIRHTAIGLN
                     YIDTYHRSGLYPLPYPTRLGVEAAGFVEAAGDAVTDLKEGDRVAYAGGMGAYAEANNV
                     PASRLVRIPDNLADETVAACLLKGLTACYLLTRTYPLAANDTMLLHAAAGGTGLIMCQ
                     WARHIGATVIGTAGSQEKAELAEHNGADHTILYREEDVVARVREITGGRGVNVAYDSV
                     GLDTYQVTLKSLAPLGMFVSFGNASGKVPPVDPQDLVSNGSLFFTRPSLAHYTLRPED
                     LRAMADQLLGLVANRTVEIHINQRYALKDLVQAHRDLESRSTSGSTIIIP"
     gene            1007..2218
                     /locus_tag="AC16UZ_04550"
     CDS             1007..2218
                     /locus_tag="AC16UZ_04550"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6245983.1"
                     /translation="MEKSDFYANARSDLPGPYHGVVVVEATTTWAGPMAGCILADFGA
                     RVIKVEHPSGEVIRVLPPYLPGSTLTVPNETVNRNKENVSLDLHSTEGREIFLKLCER
                     ADIVIENFRPGTLASWGVGYEDVAAVKPDIVYVSISGFGQFGLLSHRVGYDPIAQAFS
                     GWASLNGEPEGEPTKAPTFLGDDTAGLHGALAAMAALRHREQTGEGQHVDVSLVDGLL
                     YQSNGYPTAGALGLPLQRWANQFGIAAPANVYECTDGSVFAGVLLDTHWRVLCELLER
                     EDLADLNTMERISRREEVDPILADWCASHTAREVEEIFADLGLPATRVNTYEDVATNE
                     HVASREMLQTTRLVDGNEIPLTAPPAKFSRTPTRIRSAAPALGQHNASVYAEIGLDEQ
                     TLVNLKERGII"
     gene            complement(2386..3447)
                     /locus_tag="AC16UZ_04555"
     CDS             complement(2386..3447)
                     /locus_tag="AC16UZ_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6245984.1"
                     /translation="MIFRQLFDADSSTYTYLLGCADTRDAVIIDPVFEQHGRDCALIR
                     ELGLKVRYVLDTHVHADHVTSAWLMHSSLDAEIVLSRRYGIEGVDVAVDHGDVLAFGN
                     CSLTVRATPGHTSGCVTYVTNGQDMAFTGDCLMIRGAGRTDFQAGDVHEMWRSIREQI
                     FTLPDECLIYPGHDYAGRTVSTVGEEKRFNPRIGGDAQEEDFVGYMDNLTLPHPRLID
                     IAVPANMRLGRPENDALPEDVSWGPVVTTFAGVPEIRPDWVARHMAELYVLDVREPGE
                     FEGELGHLEGAHLIPLNQLNERISEVPNDRPVVTVCQSGKRSARAAEILIKAGYDEVA
                     NVSGGLIQWSRLSLPFLEH"
     gene            3771..4682
                     /locus_tag="AC16UZ_04560"
     CDS             3771..4682
                     /locus_tag="AC16UZ_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013624.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I family protein"
                     /protein_id="HIR6245985.1"
                     /translation="MSDVQNGLDDDRPLYWGRLALRRSVYRSALREKDSLLRSIEHYS
                     RGMGDLRLGDDVLNVLITGFDPFHLETRLDQSNPSGLAALALNGSEFGSGGGRARVCA
                     SIFPVRFEDFDDGIVERFFQQILAEREPAMVVTLSMGRDAFDLERFPGRRRSAQDPDN
                     HNVLTGASPDKPLPPGKTGSHLEGPEFLEFSLPVHAMRAADGHWPVRDNRWVTTIERG
                     EFEAGSLADLQSQLAVRGSGGGYLSNEIAYRSRLLHGQLGCSCPIGHVHTPAIQGHNA
                     ALERLMVGQIRQMLAQAAASLTPRPPS"
     gene            complement(4679..5701)
                     /locus_tag="AC16UZ_04565"
     CDS             complement(4679..5701)
                     /locus_tag="AC16UZ_04565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019471932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6245986.1"
                     /translation="MMNVNRQWVLAKRPYGMVTKDNFEYREETIPEPGDGEVLIRNLY
                     LSFDPTQRGWMEDRESYLPPVQIGEVMRAGSVGQVVESRSDDFSAGDLVQTTGGWQDF
                     TVMSAGMGPMGLRKLPEGVTPRLALGALGVTGLTAYWGMLDLGQPKAGETVLVSGAAG
                     ATGSVAGQIARIKGCRVVGIAGGPEKCAWLKDEARFDDVIDYKNEDVDRRIGELCPNR
                     VDVFFDNVGGGILEAALNHINMRARVVLCGGISAYNATEPQPGPVNLMNLVIMRARME
                     GFIVIDYMDRSHIAVPELLGWINSGEIVHQEDVQEGFENIPDTLNRLFTGRNLGKQLL
                     KIADPS"
     gene            5780..7234
                     /locus_tag="AC16UZ_04570"
     CDS             5780..7234
                     /locus_tag="AC16UZ_04570"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6245987.1"
                     /translation="MSDIAFLSATELARRIRDRELSSEELLLHYLQRVDRYNGALNAI
                     VVDIREEALMAARAADAAVARGVDVPPFHGVPMTVKESYNVAGTPTTWGNPKWRGNVA
                     FEDAESVKRLKDAGVIIFGKTNVPLALADFQSFNAIYGTTNNPFDHSRAPGGSSGGSA
                     AALAAGLTGLEAGSDIGGSIRNPAHFCGVFGHKPTWNLLWMRGHAPPGDVRASSDISV
                     IGPLGRSAADLETAVRTMAGPDDIMARGYTLSLPTLKRGGLGGLRIAIWEDDPVCPVS
                     AETKARVLAVASACRDAGAEIIEGARPDFSSEHSHETYQNLLQPTMASRVPDEEYESL
                     LEYVANLDPRDTSRTARVLRAQVARFRDWKRSHELREHLRWKWHLFFQDYDLMLTPMM
                     PTSAFPHDHRPISQRTIEVDGTSMPYMDQIFWAGLTGVAYLPSTVIPTGLDDSGLPIG
                     VQLVGPEYGDLITIGVARELETAGFRFVAPPAYA"
     gene            7247..8191
                     /gene="mvaD"
                     /locus_tag="AC16UZ_04575"
     CDS             7247..8191
                     /gene="mvaD"
                     /locus_tag="AC16UZ_04575"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HIR6245988.1"
                     /translation="MGTTARAHPNIALIKYWGKAPGQENIPATPSLSITLDSLVTETH
                     VATGPSRDELYFDDEPVADRKVLDWLEVLRQRYDIPPLEIRTANNFPTAAGLASSASG
                     FAALAVALDAQCGLQLDAATLSDLARRASGSAARSIFGGYVAVQGPRWVAQPVLSPEA
                     WPLKTVIAVTTTARKSVSSSEGMAISAATSPYYPGWVESTRADFTDALAAVRTRDFQA
                     LAELAEFSCLKMHGLMLGSRPGLLYWKAATVSCLHAVRALRQDGVDVFFTVDAGPQVK
                     AICKPSAESDVRAALAAVPGVEQIVVTGLGSGARVIDP"
     gene            8188..9159
                     /locus_tag="AC16UZ_04580"
     CDS             8188..9159
                     /locus_tag="AC16UZ_04580"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase family protein"
                     /protein_id="HIR6245989.1"
                     /translation="MIHASAPGKVVLWGEYAVLTGAPGLVLAVDRRASCTLTPGGDTW
                     HFETRGLPEPAVDLDVADLTGAHPPGPSGGALAWHVLRHLDSEVPAGATLTTDTASFQ
                     DGGHKLGIGSSAAVCTAVYGAFCELLGIGPSLNDALAVHRSLQGGSGSGIDVAAAYLG
                     GSLRYQLRGGARPPAAEPFDLPDGLILRFIWTGTPAQTTRHVARFEAWRAENQTSTLT
                     ELAQASAALFESGHLVDDLAEYAERLYAMDRAAGLGIYTDAHLQLSQVAKECKVVYKP
                     CGAGGGDIGVAASNNEYRVERFVSAALGLGFQIVEMEKTEHGIQLAS"
     gene            9137..>10521
                     /locus_tag="AC16UZ_04585"
     CDS             9137..>10521
                     /locus_tag="AC16UZ_04585"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009397571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HIR6245990.1"
                     /translation="MASSSPAESGSRIPNFFRMSVAERIAALHARGLLSSEDVHRLTA
                     GDHTLRLPVADKMIENVVGVFGLPMGVALNFLVNGRDYVVPLVVEEPSIVAGLSGAAR
                     LARLGGGFRASSTEPILIGQVQVVNVDDPDKAMADLLANRDEILALANSLHPKMVARG
                     GGALDIEVFHHHAPEDGREMVVLHLLVDTRDAMGANLVNTMCEGIASMVETITDGKVF
                     LRILSNLTDRAVATASVRIPAQNLEGKGFSGEDVRDGIILAADLATVDPYRAATHNKG
                     VMNGVDAVAIATGNDWRAIEAAAHAYAARDGQYRSLTRWYKNEDGDLVGEIQIPMKVG
                     TVGGSLETNPSVRINHRLLGTPGATELAGIMAAVGLAQNFAALRALSTDGIQQNHMTL
                     HARSVASTANVPEELFDTVVEALIESGEIKVWKAQEIVKNLTRKIAMPADVERSSACG
                     KVILLGEHAVV"
BASE COUNT         1895 a         3539 c         3314 g         1773 t
ORIGIN      
        1 ggggagggag catggtcggc gtctatcgca tccacgaagc aggtggtccc gaggttctca
       61 gctgggagtc ggaagagctt cccgacccga ccggcgagct ggtgcgcatc agacacactg
      121 cgatcggtct gaattacatc gacacctacc atcgcagcgg tctctaccca cttccctacc
      181 ccacccgact cggcgtggaa gcggcaggct tcgtcgaggc cgcaggggac gccgtgacgg
      241 atctaaagga aggggatcga gtggcatatg cgggcgggat gggcgcctat gcggaggcaa
      301 acaacgtgcc tgccagcaga ctggtccgta tcccggacaa cctcgccgac gagacggtcg
      361 ccgcctgcct gctcaaggga ctcacagcct gctacctgct gacgcgcacc taccccctgg
      421 ccgccaacga cacgatgctg ctccatgccg cggccggcgg taccggcctg atcatgtgcc
      481 agtgggcgcg ccatatcgga gcgacggtca tcgggacagc gggtagccag gaaaaggccg
      541 agctggccga gcacaacggt gccgaccaca cgattcttta tcgggaggag gacgtggtag
      601 ccagggtgag ggaaatcacc ggcggacgag gcgtcaacgt cgcctacgac tccgttggcc
      661 tggacaccta ccaggtgact ctgaaaagtc tggctccgct cggcatgttc gtgagcttcg
      721 gcaacgcttc cggcaaggtt ccgccggtag acccccagga tctcgtcagc aacggctcgc
      781 ttttctttac ccgaccttcc ctggcgcatt acacgctccg gccggaggac ctgcgagcca
      841 tggccgatca gctgctggga ctggtcgcaa accgaacggt cgagattcac atcaatcaac
      901 gctatgcgct caaggacctg gtccaggccc atcgcgatct ggaatcccgc agcacgtccg
      961 gttcaaccat catcattccg tagccccgat tcaggaaacc aaggtcatgg aaaagtcaga
     1021 cttctacgcc aacgcgcgca gcgatctccc cggaccgtat cacggggtcg tcgtcgtgga
     1081 ggcgaccacc acctgggcag gaccgatggc aggatgcatc ctggccgact tcggggcgcg
     1141 ggtgatcaag gtggagcacc cctccggaga ggtgatccgg gttctgcccc cgtacctgcc
     1201 aggctccacg ctcaccgttc cgaacgagac ggttaataga aacaaggaaa acgtctccct
     1261 cgacctgcac tcgaccgagg gacgcgagat cttcctgaaa ctatgcgagc gcgccgacat
     1321 tgtcatcgag aatttccgtc cagggacctt ggcttcctgg ggcgtcggct atgaggacgt
     1381 ggccgccgtc aaacccgaca tcgtctacgt ctccatcagc ggcttcgggc agttcggcct
     1441 ccttagccat cgggtcggat acgatcccat cgcacaggct ttttccggat gggcgtccct
     1501 caacggcgag ccggaaggcg agccgacgaa agcgcccact ttcctcggcg acgacacggc
     1561 cggtctgcac ggggcccttg ccgccatggc ggcgctgcgc caccgggagc agaccggaga
     1621 agggcagcac gtggacgtct ccctcgtgga tggcctgctc taccagagca acggctaccc
     1681 gacagcgggg gcgcttgggc ttccgctcca gcggtgggcc aaccagttcg gcatcgccgc
     1741 gccagccaac gtctacgagt gcaccgacgg ctccgtcttt gcaggcgttc tgctcgacac
     1801 ccactggcgc gttctctgcg agcttctgga gcgggaggat ctggccgact tgaacaccat
     1861 ggaacgcatc tcacgccgtg aggaagtcga tcccatcctg gccgactggt gcgcgtccca
     1921 cacggcgcgc gaagtagagg aaatctttgc cgatctaggc cttcccgcca ctcgggtcaa
     1981 cacctacgag gacgtggcga cgaacgagca cgtcgcctcg agagagatgc tccagaccac
     2041 tcgcctggtg gacggcaacg agattcccct gacggcgccc ccggccaaat tttcacgcac
     2101 ccccacccgg atccgcagcg ccgctccggc gctgggccaa cacaacgcct ccgtgtacgc
     2161 cgagatcggc ctggacgagc aaacgctcgt gaatctgaag gagaggggca tcatctgacg
     2221 cgatcccgac gcgcagagaa ccacctcagt tcctcgagcc gcggtcggta ctgccgcgtt
     2281 cgccgctggc gcgatggtaa acgctagggg ggattgattg gtcgggcccc gccctggctc
     2341 agggcggctc ccagatgggc gagccagcga gaccgtcccg cgtgactagt gttcgagaaa
     2401 cggcagggac agccgcgacc actggatcag acccccgctc acgttcgcca cctcgtcgta
     2461 tcccgcttta atgaggatct ccgcggcccg cgcggacctc ttgcccgact ggcagacggt
     2521 taccaccggc cggtcgttgg ggacctcgga aatcctttcg ttcagctgat tgaggggtat
     2581 caggtgcgcg ccttccagat ggccaagctc accttcgaac tcgccgggct cccgcacatc
     2641 gagcacgtac agctccgcca tgtgccgcgc cacccagtcg ggacggatct ccggtacccc
     2701 ggcaaacgtc gtcacgaccg gcccccagga cacgtcctcg ggcagcgcat cgttctccgg
     2761 gcggccgagg cgcatgttgg ccgggaccgc gatgtcgatc agtcgcgggt ggggcagggt
     2821 caggttgtcc atgtagccaa cgaagtcctc ttcctgcgcg tctcctccga tccgcggatt
     2881 gaagcgcttt tcctcgccga ccgtggagac ggttctgcct gcataatcgt gtcccggata
     2941 gatgaggcac tcatcgggca gggtaaagat ctgctcgcgt atgctgcgcc acatctcgtg
     3001 gacgtcacct gcctgaaaat cggtgcggcc tgcgccccgg atcatcaggc aatcgcccgt
     3061 gaaggccatg tcctggccat tggtcacgta ggtcacacag ccgctggtat gaccgggcgt
     3121 tgccctcacc gtcagggagc aattgccgaa ggccaggacg tcgccgtggt ccacggcgac
     3181 gtcgacgcct tcgatcccgt aacgtcgcga gagcacaatc tcggcatcga gggaactgtg
     3241 catcagccaa gcactggtca catgatccgc atggacgtgg gtgtcgagca cgtacctgac
     3301 tttcaaaccg agctctctga tgagcgcgca atcgcggccg tgctgctcga agacggggtc
     3361 gatgatgacc gcatcgcgag tatccgcgca cccgagcagg taggtatacg tggacgagtc
     3421 cgcatcgaaa agctgtctga atatcatgac gttccctcac cctaccggtg cgctcaaagt
     3481 ctaccccgcg agccgatacc tcgcatcctg acgtgtatac tccctttgcc gccgccacgg
     3541 agcgggtgcc gaatttcgcg gcgtccggac tcatcattcg tagccagggc ccttctgctc
     3601 cagaccccga aactcaacgt ctgccccggg cgattgcctc gctcccaggc gttgccgacc
     3661 ggcacctgaa tctcgccaag cagctcgagc gtcaactcgc gcgcttcact gacgccggtc
     3721 aggccgatgc gacagtcgcc gaatccgccc ggacgctctg gcgcgccgcg gtgagcgatg
     3781 tccagaatgg cctggacgac gatcgcccgc tctactgggg gcgactggca cttcggcgat
     3841 ccgtctaccg cagcgccctc cgtgaaaagg actcgctgct tcggtccatc gagcactact
     3901 cccgaggcat gggtgacctt cggctcggcg atgatgtcct caacgtgctg atcacgggat
     3961 tcgacccatt tcacctggag acccggctcg atcagagcaa cccgtccggg ttggcagcgc
     4021 tggcgctgaa cggatccgag ttcggttccg gcggtgggcg ggccagagtc tgcgcgagca
     4081 tatttcccgt caggttcgaa gatttcgacg acggcatcgt cgagcgcttt ttccagcaga
     4141 tccttgctga gcgcgagccc gccatggtgg tgacgctgag catgggccgc gacgccttcg
     4201 acctggaacg tttcccggga cggcggcgct ccgcgcagga tccggacaat cacaacgtgc
     4261 taaccggcgc gagcccggac aaacctttgc ccccgggcaa aaccgggtcg cacctggagg
     4321 gccccgagtt cctggagttc tctctcccgg tgcatgccat gcgggcggcg gacggccact
     4381 ggccggtccg cgacaaccga tgggtcacca ccatcgagcg tggggagttc gaggcgggat
     4441 cgctggcgga cctgcaatcc cagctggccg tgcgaggttc tggagggggt tacctgtcca
     4501 acgagatcgc gtaccgaagc cgtctgctcc atgggcaact cggctgcagc tgccccatcg
     4561 gccacgtcca cacccccgct atccagggcc acaacgcagc gctggagcga ctcatggtgg
     4621 gacagatccg gcagatgctg gcgcaggctg ccgccagcct gacgccccgg ccgccgtcct
     4681 agctcggatc cgcgatcttc agcagctgct tgccgaggtt acgaccggtg aacagcctgt
     4741 tcagggtgtc cggaatgttc tcgaagcctt cctgaacgtc ttcctgatga acgatctcgc
     4801 cgctgttgat ccaaccgagc agctcgggga ccgcgatgtg cgaacggtcc atgtaatcga
     4861 tgacgatgaa gccctccatg cgcgcccgca tgatgaccag gttcatgagg ttcaccggcc
     4921 ccggctgcgg ctccgtggcg ttgtacgccg atatgccccc gcagaggacg acgcgcgcgc
     4981 gcatgttgat gtgattcagc gcggcctcga ggattccgcc gccaacgttg tcgaaaaaga
     5041 cgtccacccg gttcgggcac aactcgccga tccggcggtc gacgtcttcg ttcttgtaat
     5101 cgatcacgtc gtcgaagcgc gcctcgtcct tgagccacgc gcacttctcc gggccaccgg
     5161 cgatcccgac cacccgacat cctttgatac gcgcgatctg gccggctacc gatcccgtcg
     5221 cgcccgcggc ccccgatacg agcaccgtct ccccggcctt cggctggcca agatccagca
     5281 tgccccaata ggcggtcagc ccggtcaccc cgagcgcgcc cagggcaaga cgcggtgtta
     5341 ccccctccgg cagcttgcgc agccccatgg gccccatgcc cgccgacatc accgtgaagt
     5401 cctgccagcc ccccgtggtc tgaacgagat caccggcgct gaagtcgtcg cttctgctct
     5461 caacgacctg acccacagac cccgcgcgca tcacctcgcc gatctggact ggcggcaggt
     5521 agctttcacg atcctccatc catccgcgct gagtcggatc gaaagagagg taaagattgc
     5581 ggatgagcac ttcgccatct ccgggctccg gaatggtttc ttcccgatac tcgaagttgt
     5641 ctttggtgac cattccatac ggccgtttgg ccagtaccca ttgtctgttg acgttcatca
     5701 gcggtcctcc atgcgagcgc ttgattaaat catcggcaaa ggtcagagtc accgcggtct
     5761 gcgaatctga taggggacaa tgagcgacat cgcgttcctc tcggccaccg agctcgcccg
     5821 caggatccgc gaccgcgagc tttccagcga agagctgctg ctgcattact tgcagagggt
     5881 cgaccgctac aacggcgccc tcaacgccat cgtggtggac atccgggagg aggcattgat
     5941 ggcggcgcgc gccgccgatg cggcggtagc ccgaggcgtc gacgtgcccc cctttcacgg
     6001 tgtgcccatg acggttaagg agtcatacaa cgtcgccggc acccccacga cctggggcaa
     6061 ccccaaatgg aggggcaacg tcgcgttcga ggacgccgaa tcggtgaagc gtctgaagga
     6121 cgccggcgtg atcatcttcg ggaagaccaa cgttcctctc gcgctggccg acttccagag
     6181 cttcaacgcc atctacggca ccaccaacaa cccattcgat cactcgcgcg cgccgggcgg
     6241 ctcgtcgggg gggtcggcgg cggcgctggc ggccgggctc acgggtctcg aggccggctc
     6301 cgacatcgga ggttctattc gcaatcccgc acacttctgc ggggtcttcg gtcacaagcc
     6361 gacatggaac ctcctgtgga tgcgcggaca cgcaccgcca ggcgatgtgc gagcgagctc
     6421 agacatctcc gtcatcgggc cgctgggtcg atctgccgca gacctggaaa ccgcggttcg
     6481 taccatggcc gggcccgacg acatcatggc gcgggggtac acgctgagcc tgccgaccct
     6541 gaagagaggg gggctcggag ggctcaggat cgccatatgg gaggacgacc ccgtgtgtcc
     6601 ggtgagcgcc gagacgaagg cccgcgtgct cgccgtggcg agcgcatgca gggacgccgg
     6661 tgccgagatc atcgagggcg cgcggcccga cttcagctca gagcacagcc acgagaccta
     6721 ccagaatctt ctccagccca ccatggcatc acgcgtgccg gacgaggagt acgagagtct
     6781 tctggaatat gtggccaatc tcgatccgcg ggacaccagc cggacggcgc gggtgcttag
     6841 agcccaggtc gccaggttcc gggattggaa acgcagccac gagctgcgcg agcacctgcg
     6901 ctggaagtgg cacctgttct tccaggacta cgatctgatg ctgaccccga tgatgccgac
     6961 ctcggcgttt ccccacgatc accgccccat cagccagcgc accatagaag tcgacgggac
     7021 cagcatgccg tatatggatc agatcttctg ggccgggctg accggggtgg cctacctgcc
     7081 gtccacggtc ataccgaccg gcctggacga ctcgggcctg cccatcggcg tgcagctggt
     7141 tgggccggag tacggggacc tcatcaccat cggcgtggcc agggaactgg agaccgccgg
     7201 cttccgcttc gttgcgccac cggcttacgc ctgacgggtt gcagccatgg gcacaacggc
     7261 tcgcgcgcat ccgaacattg cgctgatcaa gtactgggga aaagcgccgg ggcaggaaaa
     7321 catcccggcc acgccctcac tgtccatcac cctggattcc ctggttaccg aaacccacgt
     7381 tgccaccggg ccatcccggg acgagctcta cttcgacgac gagccggtgg cggaccgcaa
     7441 ggtgctcgac tggctcgagg tgctgcgtca acgctacgac ataccaccgc tggaaatccg
     7501 cacggccaac aatttcccaa ccgccgccgg gctggcgtcg tccgcgtccg gcttcgccgc
     7561 cctggccgtg gctttggatg cccaatgcgg cctgcagctg gatgcggcca cgctctcgga
     7621 tctcgcccgc cgcgcgtcgg gatccgccgc gcgttcgata ttcggcggct acgtcgccgt
     7681 gcaggggccg cgctgggtcg cccagcccgt tctcagcccg gaagcctggc ccctgaagac
     7741 ggtcatcgcg gtgacgacca cggcccgaaa atccgtctcg tcctccgagg gaatggccat
     7801 ttccgctgcc acctcaccct actacccggg atgggtggag agcaccaggg ccgacttcac
     7861 cgacgccctg gccgccgtca gaacgcgcga ttttcaggcg ctggctgagc ttgcggagtt
     7921 cagctgcctg aaaatgcacg gcctcatgct gggctcgcgg cccgggcttt tgtactggaa
     7981 ggccgccacc gtctcctgcc tccacgcggt tcgcgcgctg agacaggacg gcgtggacgt
     8041 cttctttacg gtggatgcgg gcccccaggt caaggcgatc tgcaaaccga gcgccgagag
     8101 cgacgtgaga gcggccctgg cagcggtgcc gggtgtggag cagatcgtcg tcacgggtct
     8161 gggttccggc gcgcgggtca tcgatccgtg atccacgcga gcgccccggg taaggtcgtg
     8221 ctctggggcg agtacgcggt gctcaccggc gcccccggac tggtgctggc cgtcgatcgc
     8281 cgggccagct gcacgctgac gccggggggc gatacctggc acttcgaaac ccggggactg
     8341 cccgagccag ccgtggatct cgacgttgcc gatctgaccg gagcgcaccc gcccggcccg
     8401 tcgggcggcg cgctggcatg gcacgttctg cggcatctgg acagcgaggt cccggccggc
     8461 gcgaccctga cgaccgacac cgccagcttc caggacggcg gccacaagct tggcatcggg
     8521 tcgagcgcag cggtttgcac cgccgtctac ggggcattct gtgagctgct cggaatcggc
     8581 ccgagcctga acgacgccct cgccgtgcac cgaagcctgc agggtggaag cggcagcggc
     8641 atcgacgtcg ccgcggcgta tctcggcggg tcgttgcgct accagctgcg cggcggggcc
     8701 cggcctccgg cggcagagcc gttcgatctg cctgacgggt tgatcctgcg cttcatctgg
     8761 acgggaaccc cggcacaaac cacccggcac gtcgcgcggt tcgaggcatg gcgggcggaa
     8821 aaccagacga gcaccctgac cgagctcgcc caggcatctg ccgccctgtt cgaaagcggg
     8881 catctcgtcg acgacctggc cgaatacgcc gagcgactct acgcaatgga ccgcgccgcc
     8941 ggattgggca tctacaccga cgcgcatctg cagctgtcgc aagttgcgaa agaatgcaaa
     9001 gtagtctaca aaccctgcgg cgccggtggg ggcgacatcg gcgtcgcagc cagtaacaac
     9061 gagtatcggg tcgagcgatt cgtttcggcc gccctcgggt tgggcttcca gatcgtagaa
     9121 atggagaaaa ccgaacatgg catccagctc gccagctgaa agtggatcgc ggatacccaa
     9181 ttttttccga atgagcgtgg ctgagcgcat cgccgcgctt catgcccggg ggctcctgag
     9241 ttcggaagac gttcatcggc tgaccgccgg tgaccacacc ctgcgactgc ccgtggcgga
     9301 caagatgatc gagaacgtcg tcggcgtttt cgggctaccc atgggcgtcg cgctcaactt
     9361 tctggtcaac ggccgcgact acgtcgtccc cctggtcgtc gaagagcctt ccatcgtcgc
     9421 cgggctttcc ggggccgcac ggctggcccg gctcggcggc gggttccgag ccagcagcac
     9481 ggagccgatc ctcatcgggc aggtccaggt ggtcaacgtc gacgaccccg ataaggccat
     9541 ggccgacctg ctcgccaatc gggatgaaat tctcgccctg gccaacagcc tgcatccgaa
     9601 gatggtcgcg cgcggcgggg gtgccctgga catcgaggta ttccatcatc acgcgcccga
     9661 agacgggcgc gagatggtcg ttctgcacct gctggtggac acccgcgacg ccatgggcgc
     9721 caacctggtc aacaccatgt gcgagggtat cgcctcgatg gtcgagacca tcaccgacgg
     9781 caaggttttc ctcaggatcc tttccaacct gaccgaccgc gccgtggcca cggcctccgt
     9841 gcgcatacca gcgcagaacc tggaggggaa aggattcagc ggcgaggacg tccgggacgg
     9901 catcatcctc gccgcggacc tggccacggt ggatccgtac cgcgccgcca cgcacaacaa
     9961 gggtgtcatg aacggggtgg atgccgtggc catcgccaca ggcaacgact ggcgcgccat
    10021 cgaagccgcc gctcacgcgt acgcggcccg cgatggccag taccgctcgc tgacacgctg
    10081 gtacaagaac gaggacggcg acctcgtcgg ggaaatccaa atccccatga aggtcgggac
    10141 cgtgggcggc tcgctggaaa ccaaccccag cgtccgcatc aatcaccggc tcctgggcac
    10201 cccgggggcg acggagctcg ccggcatcat ggctgcagta gggctcgcgc agaactttgc
    10261 cgctctgcgc gcgctgtcga cagatgggat acagcagaat cacatgaccc tgcacgcccg
    10321 cagcgtggcg agcacggcca acgttcccga ggagctgttc gacacggtgg tcgaagcgct
    10381 catcgagtca ggcgagatca aagtctggaa ggctcaagaa atcgtaaaga acctgacccg
    10441 gaagatcgcc atgcccgcag acgtggagcg aagctcggcg tgtggcaagg tgatactgct
    10501 gggtgagcac gccgtggttt a
//
LOCUS       DBNHOX010000207         1586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1752569, whole genome shotgun sequence.
ACCESSION   DBNHOX010000207 DBNHOX010000000
VERSION     DBNHOX010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1752569"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="AC16UZ_04590"
     CDS             complement(<1..154)
                     /locus_tag="AC16UZ_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR6245991.1"
                     /translation="MTTIAGADAPFEICWDHLGIPHVYASTVADAYRGMGYVEGYERL
                     WQIHLSC"
     gene            298..1098
                     /locus_tag="AC16UZ_04595"
     CDS             298..1098
                     /locus_tag="AC16UZ_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6245992.1"
                     /translation="MLKTENYRTMLVERRENGVGVLTLNRPERLNAVNGTMHSELARF
                     SLDFASDPDLRVLVVTGAGRAFCAGGDFSPDDPVGSNPDGTDMWAEARQIVDRMLECE
                     KPIISAVNGYAMGLGATVALLADVVVVSPKAVIADTHVAMGIGAGDGGQLIWPHLIGV
                     NRAKYYLMSGDRIDGREAAEMGLASFCVEDHDDLMPKALEIAHRLAAGAPQAIAASKA
                     AVNAYLRSVSAMVMPIGLRYEQQTMHTSDHREAVAAFQEKRPPKFTGR"
     gene            1224..>1586
                     /locus_tag="AC16UZ_04600"
     CDS             1224..>1586
                     /locus_tag="AC16UZ_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6245993.1"
                     /translation="MLAPRVIGNSSVNSVGLGCMNVSHAYGPAQPEEDAIRLFQHALD
                     TGYDFFDTATIYGLGTNEALLGKAIAHRRSEFFLASKCVMGMRDGKRVLDGRPENLKS
                     QCDESLKRLQTDVIDLYYM"
BASE COUNT          297 a          467 c          528 g          294 t
ORIGIN      
        1 ggcagctcag gtgaatctgc cacaggcgct cgtagccctc cacgtagccc atgccccggt
       61 aggcgtcggc gaccgtggag gcatatacat gggggatgcc gaggtggtcc cagcagatct
      121 cgaacggggc atctgctccg gcgatggtcg tcatgctggt cttcctctat cgcgcgctcg
      181 ggtgtgggtc caggggatga ttcattctct gtcggatcgt ttcgccgtag agtagcggat
      241 cgaagatggt ggtgcaggtt tcaacaaagc tggctgacaa ctcggaggac atctcacatg
      301 ctcaagacgg aaaactatcg gaccatgctc gtcgagagac gggagaacgg cgtcggtgtg
      361 cttacgctca atcgccccga gcgtctcaac gcggtgaacg gcaccatgca ttcggagctt
      421 gcgcgcttca gcctggactt tgccagcgac ccggacctgc gcgtgctggt cgtcaccggc
      481 gcgggccggg cgttctgcgc gggaggcgat ttcagccccg acgacccggt gggttcaaat
      541 cccgatggga ccgacatgtg ggcggaagcc cggcagatcg tcgaccgcat gctggaatgc
      601 gaaaaaccca tcatctcggc cgtcaacggg tacgccatgg ggcttggcgc taccgttgcg
      661 ctgctggccg acgtcgtggt cgtgtctccc aaagccgtga ttgcggacac ccacgtggcc
      721 atgggcattg gcgcgggtga cgggggccag ctcatctggc cgcacctgat cggtgtgaat
      781 cgcgccaagt actacctgat gtcgggggat cggatcgacg ggagggaggc cgcagaaatg
      841 gggctcgcca gcttctgcgt ggaggatcac gacgacctca tgccgaaggc gctggagata
      901 gcgcaccggc ttgccgctgg cgcgcctcag gcgatcgccg cgtcgaaggc ggcggtgaac
      961 gcctatcttc gctccgtctc ggcgatggtc atgcccattg gtctgcgcta cgagcagcag
     1021 accatgcaca cgagcgatca tcgggaggcc gtggcggcgt ttcaggaaaa gcggccgccg
     1081 aagttcaccg ggcgatagtc cggttcaaag tctctccacg atggtctgaa gcatactggg
     1141 gggtcatttg gccggcgcag ttgttactgc ggctgagacc cgaatacact gacaccccgt
     1201 cactaagatc tggaggaagg cggatgctcg cgccccgcgt cattggaaat tccagcgtta
     1261 actccgtcgg gctcggctgc atgaatgtca gccatgctta cggcccggcc cagcccgagg
     1321 aggacgccat acggctgttc cagcacgcgc tggataccgg ctacgacttc ttcgacacgg
     1381 caaccatata cgggctcggg acgaacgaag cgctgctcgg gaaagccatc gcccatcggc
     1441 gatcggagtt tttccttgcc agcaagtgcg tcatgggcat gcgcgatggc aagcgggtgc
     1501 tcgacggtcg accggagaat ctgaagtcgc agtgcgatga gagcctgaag cggctgcaga
     1561 cggacgtcat cgacctgtat tacatg
//
LOCUS       DBNHOX010000208         4263 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_338754, whole genome shotgun sequence.
ACCESSION   DBNHOX010000208 DBNHOX010000000
VERSION     DBNHOX010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_338754"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2256)
                     /locus_tag="AC16UZ_04605"
     CDS             complement(<1..2256)
                     /locus_tag="AC16UZ_04605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HIR6245994.1"
                     /translation="MDATGFILQATYRIHAGVPVIYLFGRLESGETFLVRDHRQRPHF
                     YVRQEDARRAEALDGIALEASAARTFSGDAVCRVTVPTPSDAPPARERLHGLGIDTFE
                     ADVRFAMRYLIDRGIRGGCRIRGAASRGEGVGWVLDDPEVGAAEAGIEPVVLSFDIET
                     DAKADRLLGIAVYGAGVDEVLIVDPERRPMPERARGLPSERAVLEAFCQLVRDVDPDV
                     LTGWNVVDFDLTVLGRVAARVRHPFQLGRQAGALRIRPAQGYFGSGNATIPGRLVLDG
                     MDLLRGAFVRMEEYSLDAVAREVLGEGKTLDGDVRDRAGEIMQRYRQDLPGFARYART
                     DARLALDIVTRLDLMNLAFARSRLTGMTPDRVAASIASFDFLYLSALGKRGLVAPTVR
                     SDDSRVHAAQSGGQVFEPVTGVHRNVMVFDFKSLYPSIIRTFNVDPLGFVPSGDAEPD
                     VIRLVNGAGFRRDPAILPRMLDDLFPRREAAKTAGDSVASQAIKILMNSFYGVLGTPA
                     CRFHNVEIANAITGQGRHLLQWSKRWFETRGFDVLYGDTDSLFVRSRQGDEDAALAEA
                     RDLAARLNADIAAEVLERWGVVSRLELEFEKLYAKLFLPSVRHGAAGARKRYAGLPVG
                     AEEVEFVGMEVVRRDWTELAKRVQRELYARLFSGAAVDDYLAGVVAQLRRGELDELLV
                     YRKGLRKRLDSYTASNPPHVAAARKSGEPPGRTVAYLVTSAGPEPLDALQHEPDREHY
                     VDKQIKPVAEPV"
     gene            2388..3086
                     /locus_tag="AC16UZ_04610"
     CDS             2388..3086
                     /locus_tag="AC16UZ_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6245995.1"
                     /translation="MSRPEGSILLVEDNADLAETVGAFLEAKNYVVDYAADGLLAVQL
                     ATSEAFDAIVLDIMLPGIDGIEVCRRLRRDARLSTPIIMLTARDQLQDKLDGFDVGAD
                     DYLVKPFDMPELVARIEALIRRGRGVSAAYNVGELSMDLDTMSVFRGDTEIPLSRTLF
                     DILKILLREHPKVVPRETIERELWGDDLPDSDTLRSHLYNLRKAVDRPFNEPLIETLA
                     GRGYRVRVPEPDAG"
     gene            3166..4116
                     /gene="folE2"
                     /locus_tag="AC16UZ_04615"
     CDS             3166..4116
                     /gene="folE2"
                     /locus_tag="AC16UZ_04615"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HIR6245996.1"
                     /translation="MSTPKPVELASMPDIAGRPHAKPQGTLDWVGMSDIHQPILIRAG
                     NVNQRVQARVQVYVDLNDPHAKGIHMSRLYLLLDEHARTRPLSVAGLKMLLGSMHSSH
                     LDLSSRAFVQFDFEYELRQMALLSDNAGWSSYPITVKGTLADGKMTLELGVEILYSST
                     CPCSAALARQLIQQAFADRFDSGRPIDFADVNAWLGTEQGIVATPHSQRSVAKVITQL
                     ADDVTELPIAALIDTLETAIGTPVQAAVKRQDEQEFALRNGQNLMFCEDAARKLQAAL
                     NDDPSFNDFWLRVEHHESLHPHDAVSIVTKGIDGGYLPIP"
     gene            complement(4118..4246)
                     /locus_tag="AC16UZ_04620"
     CDS             complement(4118..4246)
                     /locus_tag="AC16UZ_04620"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6245997.1"
                     /translation="MRGHGLGLAIVKRLAHQAGWSISAHSKPGEGTRVEVAFFPDD"
BASE COUNT          779 a         1493 c         1328 g          663 t
ORIGIN      
        1 aacgggctcg gccaccggct ttatctgctt gtcgacgtaa tgctcccgat cgggctcatg
       61 ctgcagcgcg tcgagaggct cggggcccgc gctggtcaca aggtaagcca cggtgcgccc
      121 aggcggctct cccgactttc gcgccgcagc cacgtgggga gggttgctcg ccgtgtagct
      181 gtccagtcgc ttcctgagcc ctttgcgata gaccagaagc tcgtccagct cgccgcgacg
      241 cagctgcgcg acgactccgg ccaggtagtc atccacggcg gctccggaga agagcctggc
      301 atacagctcc cgctgaacac gcttggcaag ctcggtccag tcccggcgga cgacctccat
      361 gcccacgaac tccacctctt cggccccgac aggcaacccc gcatagcgct ttcgcgcgcc
      421 cgccgcaccg tgacgaacag acggcaggaa gagcttggcg tagagctttt caaattccag
      481 ctcgaggcgg ctgacgaccc cccagcgctc gagcacctcg gcggcaatgt ccgcattgag
      541 ccgcgcggcg agatccctcg cttccgcaag ggcagcatcc tcgtcgccct gccgcgaccg
      601 cacgaaaagg ctgtcggtgt cgccgtagag cacgtcgaag cccctcgtct cgaaccagcg
      661 tttcgaccac tgcagcagat ggcggccctg accggtgatg gcgtttgcga tctcgacgtt
      721 gtgaaaccga caggccgggg tgcctagcac accgtagaaa gagttcatga ggatcttgat
      781 cgcctgtgag gcaacgctgt ccccagctgt cttcgcagcc tcccggcgcg gaaacaggtc
      841 atcgagcatg cgcggcagga tggccggatc ccgccggaat ccagcgccgt taacgagccg
      901 gatcacgtca ggctccgcgt cgcccgatgg cacgaacccc agaggatcga cgttgaaagt
      961 tcggatgatg ctcgggtaga ggcttttgaa atcaaagacc atgacgttgc gatgcacgcc
     1021 ggtcacgggc tcgaaaacct ggcctcccga ctgcgcagcg tggacgcggc tgtcatcgga
     1081 acgcaccgtg ggcgcaacca ggccccgctt gcccagagcg gacaggtaaa ggaagtcgaa
     1141 agaggcgatg ctggcagcca cccgatccgg cgtcatgccc gtcagccggc tgcgagcgaa
     1201 agccaggttc atgagatcga ggcgagtgac gatgtccagc gccaggcgcg catccgttcg
     1261 cgcatagcgc gcaaaccccg gcagatcctg gcggtagcgc tgcatgatct cccccgcacg
     1321 gtcgcgcacg tcgccatcca gcgttttgcc ctcgccgagg acctcccgcg ccacggcgtc
     1381 cagcgaatat tcttccatcc gcacgaacgc cccgcgaagc aggtccatgc cgtcgagcac
     1441 cagccggccg ggaatggtcg cgttgccgct cccgaagtaa ccctgggcgg ggcggatgcg
     1501 gagcgcgccc gcctgccgac cgagctgaaa cggatggcgc actctggcgg caaccctccc
     1561 gagcaccgtc aggtcgaaat cgaccacgtt ccagcccgtc agcacgtccg ggtcgacgtc
     1621 gcgaacgagc tgacagaaag cttccagcac cgcccgctcg gagggcaagc cccgggcacg
     1681 ctcgggcatg ggccgtcgct cggggtcgac gatgaggacc tcgtccacgc cggcgccgta
     1741 gacggcgatg ccgagcagcc ggtccgcctt ggcgtcggtc tcgatatcga aggaaagaac
     1801 caccggctcg ataccggctt cggcggcgcc gacctcagga tcgtcgagca cccagcctac
     1861 cccttctccg cggcttgccg ccccgcggat gcggcagccg ccgcggatgc cccggtcgat
     1921 cagatagcgc atggcgaagc ggacgtccgc ctcgaaggtg tcgatcccaa ggccgtgaag
     1981 ccgctcgcgc gcgggcggcg catctgaggg ggtgggcacg gtcacccgac acaccgcgtc
     2041 cccggagaac gttctggcgg cggacgcctc gagggcgatg ccgtctaagg cctcggctcg
     2101 ccgggcatcc tcctgccgca cgtagaaatg aggccgctgc cgatggtcac gcaccagaaa
     2161 cgtttcgccg gactccaggc ggccgaacag gtaaatcacg ggcacccccg cgtgaatgcg
     2221 gtaggtggcc tgcaatatga aaccggtcgc atccatggat ctatcggctc gacgccatgc
     2281 gagcgcccat gtcgggggga cggatcattg tgcccattgc cgcgagccga aaccaagggg
     2341 cttcgagggc gttgcggccg gcgtgaccct gcctctaaga tgcgccgatg agtcgacccg
     2401 aaggcagtat cctcctcgtg gaagacaacg cggacctggc agagacggtc ggcgcgttcc
     2461 tggaggcaaa gaactacgtg gtggactacg ccgcggacgg gctgctcgcc gtgcagctcg
     2521 cgaccagcga agccttcgac gccatcgtgc tggacatcat gctgcccggc atcgacggca
     2581 tagaggtctg ccggcgcctg cgcagggatg ccaggctctc cacccccatc atcatgctca
     2641 ccgcccggga ccagcttcag gacaagctcg acggcttcga cgtcggcgcg gacgactatc
     2701 tggtcaaacc cttcgacatg ccggagctgg tcgcgcgcat cgaggcgctc atccgccgcg
     2761 gccgcggggt gagcgcagcc tacaacgtgg gcgagctgag catggatctg gacaccatgt
     2821 ccgtgttccg gggcgacacc gagatccccc tgtcgcgtac gctgttcgac atcctcaaga
     2881 tcctgctgcg cgagcacccg aaggtggtcc cacgcgagac catcgagcgg gaattgtggg
     2941 gagacgacct tcccgacagc gacaccctgc gcagccacct ctacaacctg cgcaaggccg
     3001 tcgaccggcc gttcaacgag ccgctcatcg agactctggc aggccgcgga taccgcgtgc
     3061 gggttcctga gccagacgcc ggctgacggg caacgccttt cacacccctt tcaccggcgc
     3121 ccctctagct tgatctcatc agatcattga tcgaggggat aacccatgag cacaccaaag
     3181 cccgtcgagc tggcctccat gcccgacatc gccggacgac cccatgccaa gccgcaaggc
     3241 accctggact gggtgggcat gagcgacatc catcagccca tcctgatccg ggccggcaac
     3301 gtcaaccaac gcgttcaggc cagggtccag gtgtacgtgg acctgaacga cccccatgcc
     3361 aagggcatcc acatgtcccg gctgtatctt ctgctcgacg agcacgcccg cacccgtccc
     3421 ctgtccgtcg cggggctgaa aatgctgctc ggctccatgc acagctctca tctcgacctg
     3481 agcagccggg cgttcgtgca gttcgacttc gagtacgagc tgcgccagat ggcgctgctt
     3541 tcggacaatg ccggctggag ctcctacccc atcaccgtga agggcaccct ggccgacggc
     3601 aagatgaccc tggagctggg ggtggagatt ctctactcga gcacctgccc ttgctcggcc
     3661 gcgctggcca ggcagctgat ccagcaggcg tttgccgacc gcttcgacag cggccggccc
     3721 atcgacttcg ccgacgtcaa cgcgtggctc ggcacggagc agggcatcgt cgccacgcct
     3781 cacagccaga ggagcgtggc aaaggtcatc acccagctgg ccgacgacgt gaccgagctc
     3841 cccatcgcgg ccctcatcga cacgctggag accgccatcg gcacgccggt tcaggccgcc
     3901 gtgaaacgcc aggacgagca ggaattcgcc ctgcgcaacg gtcagaatct gatgttctgc
     3961 gaagatgccg cgcgcaagct gcaggccgcg ctcaacgacg atccgtcctt caatgacttc
     4021 tggctgcgcg tcgagcacca cgaaagcctt cacccccacg acgcggtgag catcgtcacc
     4081 aaaggcatcg acggcggcta cctgcccatc ccctgaccta gtcgtcgggg aagaaggcca
     4141 cttctacccg ggtcccttca ccaggcttgc tgtgggccga gatgctccag ccggcctggt
     4201 gggccaaccg cttcacgatg gctaggccca ggccgtggcc tcggatcgcc cctctgctct
     4261 cgt
//
LOCUS       DBNHOX010000209         1640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1752617, whole genome shotgun sequence.
ACCESSION   DBNHOX010000209 DBNHOX010000000
VERSION     DBNHOX010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1752617"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1264
                     /locus_tag="AC16UZ_04625"
     CDS             86..1264
                     /locus_tag="AC16UZ_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6245998.1"
                     /translation="MSGRGEGPLPIPETPAALTPEWLTEALRRGGILDDQKVVDIEAE
                     VLGEGVGFLGDLLRLSLSYDRPGNLPDSLIAKLPKLDNRAMGEMLGAYERENCFYMEM
                     AGSLPLSTPRMYYGDFDRDRASEKQEDILRLFDRMPRFLLSFLPALGLRIAAAKKRRY
                     ILLLEDLSDAEPGDQVAGVSVRRCERALRAIARAHAACWGAELVDAFWLLPHDIDARM
                     RHSMFKGSRSTFSDFFGPELEARLEPYLRRLPDQGLDLIRRLNQAPTTLLHCDLRLDN
                     LFFREDEVIVIDWQLVRRGPAVYDVAYFLSSALDEAAGRAVVEGLLEAYLEELQAGGV
                     DGYTMDELLRDYRIALRVVLMTLSTVDQVELGDGRGIDLMRGWISRLHGRLAEMGEPG
                     "
     gene            1440..>1640
                     /locus_tag="AC16UZ_04630"
     CDS             1440..>1640
                     /locus_tag="AC16UZ_04630"
                     /inference="COORDINATES: protein motif:HMM:NF039725.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6316 family protein"
                     /protein_id="HIR6245999.1"
                     /translation="MPGKLPQWFRADEVYAYSGQWYFGSREGLHIGPYADREVAKAKG
                     TQVAKRLRAMDEAHQLKFVRKVL"
BASE COUNT          269 a          510 c          566 g          295 t
ORIGIN      
        1 gtctcgagcc tccgcttcag gtgggcaggc agacggtgga gccggattac accatcaaca
       61 gccttgcgga gatcaggggg ctgatatgag cgggcgcgga gagggcccgc tgccgattcc
      121 ggaaacccca gccgcgctga cgcccgagtg gctgacggag gcccttcgcc ggggcgggat
      181 tctggacgat cagaaggtcg ttgatattga ggccgaggtg cttggcgagg gcgttggctt
      241 cctgggtgac ctgctgaggt tgtccctcag ctacgatcgc cccgggaacc ttcctgactc
      301 gctgatcgcc aagctgccga agctggataa ccgcgccatg ggggagatgc tcggtgcgta
      361 cgagcgcgaa aactgctttt acatggaaat ggcggggagc ctgccgctga gcacccctcg
      421 catgtattac ggcgatttcg accgcgacag ggcatccgag aagcaggaag acattctccg
      481 cctcttcgac cgcatgccgc ggtttctcct ctcgttcctt cccgcgctgg ggctgcggat
      541 cgccgcggcc aagaagcggc gctacattct gctgctcgaa gatctcagcg atgccgagcc
      601 gggcgatcag gtggcgggcg tcagcgtgcg acgctgcgaa cgggctctgc gcgccattgc
      661 ccgcgcccac gccgcgtgct ggggagcgga gctcgtcgac gcgttctggc tgctccccca
      721 cgacatcgat gcgcgcatgc ggcacagcat gttcaagggc tcgcgatcga cattctcgga
      781 tttcttcggc cccgagctgg aggcgcgcct ggaaccctat ctgcgacgcc ttcccgacca
      841 gggcctggac ctgatccgcc gcttgaacca ggcaccgacc acgctgcttc actgtgacct
      901 gcgactggac aatctgttct tccgtgaaga cgaggtgatc gtcatcgact ggcagctcgt
      961 ccggcggggc ccggcggtgt acgacgtggc ctactttctc tccagcgccc tggacgaggc
     1021 ggccggccgc gcggtcgtgg agggcttgct cgaggcttac ctggaagagc tccaggcggg
     1081 cggcgtggac gggtacacga tggacgagct gctgcgcgac tatcgcatcg ccctgcgcgt
     1141 cgtgctgatg acgctttcga cggtggacca ggtggaactt ggcgacggtc gtggcatcga
     1201 cctgatgcgt ggctggatct cccgcctgca cggccggctc gcagaaatgg gagagccggg
     1261 gtgaatcagg tttgcgcggc cgtcgtcgtg agggcccgcg cacggcgacg gggcctgttg
     1321 cccctctccg agccggccgg aagtttgcgc tgcgtcacac actgaacgcg acgcagctcg
     1381 cccggcgaac gaggtcgcga cgttagaatc gggtgatcga gtccacgttg gagcccagcg
     1441 tgcccggaaa actgccgcag tggttcagag ccgatgaggt ttatgcctac agcggtcagt
     1501 ggtatttcgg ctcgcgtgag gggcttcaca tcgggcccta tgcggatcgg gaagtcgcca
     1561 aggcgaaagg cacccaggta gccaagcgcc tgcgcgcgat ggatgaggcg caccagctca
     1621 agttcgtccg caaggtcctg
//
LOCUS       DBNHOX010000210         1885 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_274526, whole genome shotgun sequence.
ACCESSION   DBNHOX010000210 DBNHOX010000000
VERSION     DBNHOX010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_274526"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="AC16UZ_04635"
     CDS             complement(<1..482)
                     /locus_tag="AC16UZ_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246000.1"
                     /translation="METEHQTGCPYRHADEIDFMDPVVQENWFDAYDIIREESPAYFM
                     PQIGMYVLTRYDDIEHVLRRPTQFTVGPDVQSTEPLIKHPEALALYEEKGWRRYTPLG
                     ENLPKHAHYRGLVDPALTAAAIRKRESFVRATVNELIDEWIDEGEIEFIDRFAEPLPM
                     "
     gene            593..1039
                     /locus_tag="AC16UZ_04640"
     CDS             593..1039
                     /locus_tag="AC16UZ_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6246001.1"
                     /translation="MIVGVHHVAIGVPDFETGLAFYRDVLGFEVLSASELSGNELADR
                     AIGLPDVRAQVAMLKAPNIYIELWSYSNPEPDDRRQRPCDFGYPHFALQVRDMQTEYA
                     RLSAAGMEFVGEPVELGGSAAIYGKDPFGNVIEIYEIRDPEIAQLS"
     gene            1128..1352
                     /locus_tag="AC16UZ_04645"
     CDS             1128..1352
                     /locus_tag="AC16UZ_04645"
                     /inference="COORDINATES: protein motif:HMM:NF016231.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP1242/YdcH family protein"
                     /protein_id="HIR6246002.1"
                     /translation="MFEYEQDAVQALLKANDRFQDLYSQHAALKIQVHEAEIGVHPMD
                     DLTLNQLKRQKLLAKDKMAHMIQKYQRRHA"
     gene            complement(1392..>1885)
                     /locus_tag="AC16UZ_04650"
     CDS             complement(1392..>1885)
                     /locus_tag="AC16UZ_04650"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246003.1"
                     /translation="GSELNRAYDEATVEEWDRVQDTDLKGPWLMCKHLVPRMVERGSG
                     SVINVSSLNGLVGFPLSTAYGGAKGGLVVFTRDMAIELASSGVRFNCVCPGVIETPMM
                     ERWTDLMPDKGEAQEMLRGVMPIGRMGRADEVAGAILFFASDDSSLCQGAVLSVDGGF
                     TAQ"
BASE COUNT          377 a          614 c          562 g          332 t
ORIGIN      
        1 atcatcggca gcggctcggc gaaccggtcg atgaactcga tctcgccctc gtcgatccac
       61 tcgtcgatga gctcgttgac ggtagcgcgc acgaagcttt cacgtttcct gatggccgcc
      121 gccgtcagcg cagggtccac caacccacgg tagtgggcgt gcttgggcag gttctcacca
      181 agcggcgtgt agcgacgcca gcctttttcc tcgtatagcg cgagcgcctc gggatgcttg
      241 atgagcggtt cggtgctctg cacgtccggg cccaccgtga actgggtcgg ccggcgcagc
      301 acgtgctcga tgtcgtcgta gcgggtcagc acgtacatgc cgatctgggg catgaagtag
      361 gcgggcgact cctcacgaat gatgtcgtag gcgtcgaacc agttctcctg caccaccgga
      421 tccatgaaat cgatctcgtc cgcgtgccgg tacggacacc ccgtctgatg ctcggtctcc
      481 atgatgctcc cctctccctt aaaaccctgc caactataca atttatacaa ttgcgccccg
      541 aagagacggt accatcaagg ccctcagcat tcagcaacgc aaggggatag caatgatcgt
      601 aggcgttcac cacgtggcca tcggcgttcc ggatttcgag acgggcctgg ccttttatcg
      661 ggacgtgctc ggtttcgagg tcttgagcgc aagcgagctc tccggaaatg agctcgccga
      721 ccgggccatc gggctgcccg acgtgcgggc ccaggtcgcc atgctgaaag caccaaacat
      781 ctacatcgag ctgtggagct acagcaaccc ggagccggac gacagacgcc agcgcccgtg
      841 cgacttcggc tacccccact tcgcgctgca ggtcagggac atgcagaccg agtacgcaag
      901 gctctcggca gccggcatgg aattcgtcgg cgagccggtg gagctcggcg ggtccgccgc
      961 catctacggt aaggacccgt tcggaaacgt catcgagatc tacgagatcc gcgatcccga
     1021 aatcgcccag ctgagctgac gacctccgcc gaaacagccc cgcagtcttg taagccacgg
     1081 aatttttgac tagtttcaga cgtgcacaac ctgggaaggg gtgaagcatg ttcgagtacg
     1141 aacaggatgc ggtgcaggcg cttctgaaag cgaacgaccg ctttcaggac ctctacagcc
     1201 agcacgctgc gctgaagatc caggtccacg aggcggagat cggcgtccat cctatggatg
     1261 atctgaccct aaaccaactc aagcgccaga agctcctcgc caaggacaag atggcgcaca
     1321 tgatccagaa ataccaacgc cggcacgcct gaccccgacc cgcggggcct gccgacgtcc
     1381 atctgcgcgg cctactgggc ggtaaagcct ccgtccacgg agagtacggc gccctggcag
     1441 aggctggagt cgtcggaggc gaagaacaga attgctccag caacctcatc cgcccggccc
     1501 atgcggccga tgggcatgac gccccgcaac atttcctgag cctctccctt gtcgggcatc
     1561 agatctgtcc agcgttccat catgggggtc tcgatgacgc ccgggcagac acagttgaaa
     1621 cgcaccccgg agctggcgag ctcgatggcc atgtcgcggg tgaagaccac cagaccgccc
     1681 ttggccccac cgtaggcggt cgacagagga aaccccacca ggccgttgag cgaggatacg
     1741 ttgatcacgc tgccgctgcc gcgctccacc atccggggga ccaggtgctt gcacatgagc
     1801 cagggaccct tcagatcggt gtcctgaacc cggtcccatt cctcaacggt ggcctcgtcg
     1861 taggcgcggt tgagctccga gcccg
//
LOCUS       DBNHOX010000211         3163 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1366932, whole genome shotgun sequence.
ACCESSION   DBNHOX010000211 DBNHOX010000000
VERSION     DBNHOX010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1366932"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="AC16UZ_04655"
     CDS             complement(<1..529)
                     /locus_tag="AC16UZ_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6246004.1"
                     /translation="MKLVTYDDGRADRLGALLPDGGLVDLAAAAAAGSVAARPFGSML
                     SLIEAGPVAWEQARELAAAPPANARREPEQCRLLAPLPVPPQIRDFMCFEKHVIQAFA
                     AGLRMRAARASSQSEREQLTTQADAQKPPEAWYKQPLYYKANRFAVSGPDETIDWPAY
                     SRIMDYECELACVVGA"
     gene            complement(612..1751)
                     /locus_tag="AC16UZ_04660"
     CDS             complement(612..1751)
                     /locus_tag="AC16UZ_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6246005.1"
                     /translation="MADAGRRAIRGLSLDADKRSCIVAVPLWSLPTAAMSPEQRARTL
                     ELARGDAPEPGGYLELLGGILWIRLPIPGALQHINVWLVPGNKGWFLVDTGMKTRVVR
                     EAWESLEGRLPLARELEFVLVTHHHPDHFGMARWLSERFEVGVTMHDMAAAAAVSGME
                     APEAGPVYPSDGFMERLGIELDAAMQSILRGGSYRAIVSGRPQITTLRAEATLTGTDG
                     LWRTSVHEGHAPGHVCLHDDASRVLISGDQLLPTISSNVSLYPSNEQGDPLGDYLVSL
                     ERLARLPEDTLILPAHGRPFMSLHARIEALRQEHAGRLQQIRDVCTAGQSTSTVTASL
                     FRMDRLDALNRLLATTETLAHLRHLELMGDVERRGRGVALRWARP"
     gene            complement(1883..2284)
                     /locus_tag="AC16UZ_04665"
     CDS             complement(1883..2284)
                     /locus_tag="AC16UZ_04665"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6246006.1"
                     /translation="MAEAPALFESRLAALSQPTRLRIVEVVAQGGPEGTAAGRIARAV
                     HCPASTLSFHLKELSQAGLLVATPDGRFIRYSVKPESFADLATFISALPGRPAKAVAN
                     ETPSRRQARQRAKRGTKDKTKQESQLSIFDD"
     gene            2543..2896
                     /locus_tag="AC16UZ_04670"
     CDS             2543..2896
                     /locus_tag="AC16UZ_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246007.1"
                     /translation="MNVAWESFHRATNELASSASIKHRLTAAFSNHLKDLEPDSLPPE
                     LRPRFEELHASLSRVRPLRGETAVWATVRKMSNDEAEIVARRIVELLGELARHREQLP
                     KTSGRPVLSLYAADG"
     gene            complement(2903..>3163)
                     /locus_tag="AC16UZ_04675"
     CDS             complement(2903..>3163)
                     /locus_tag="AC16UZ_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246008.1"
                     /translation="EVILILTTLHARDPSLIEFQALYAQACEKPIIVMEPFGTGETVP
                     AKLREVADEVVSWNTREMADAIRRQARHEETTRWDTIEFKLD"
BASE COUNT          548 a         1029 c         1040 g          546 t
ORIGIN      
        1 ccgctcccac cacgcaggcc agctcgcatt cgtagtccat gatccggctg taggccggcc
       61 agtcgatcgt ctcgtcggga ccgctgacgg cgaagcggtt ggccttgtag taaagcggct
      121 gcttgtacca ggcctccggg ggcttctgcg cgtcagcctg cgtcgtgagc tgctcgcgtt
      181 cactctgcga cgaagcgcgg gccgcccgca tgcgaagccc cgcagcgaat gcctggatga
      241 catgcttctc gaagcacatg aagtcgcgga tctgcggcgg aaccggaagc ggcgccagca
      301 ggcggcactg ttctggctca cgccgggcgt tggcgggcgg ggccgccgcg agttccctcg
      361 cctgctccca ggcaacgggg cccgcctcga tcagggagag catcgagccg aaaggcctgg
      421 cagccacact gccggctgca gcggctgcgg cgagatccac gaggccgccg tcgggcagca
      481 gggcccccag acggtcggcc cggccgtcat cgtaggtcac gagcttcatg gcaggaatat
      541 cgggcgcccc cgcccaaaat gcaaacccgg gggagcgcgc cgctgtgggc tgcggagtgt
      601 ccggaccggg gctagggacg ggcccatctc aacgcgacgc cacgaccacg ccgctcgacg
      661 tcgcccatga gctccaggtg acggaggtgc gcaagcgttt cggtcgtggc gagcaagcgg
      721 ttgagcgcgt caagcctgtc catccggaag agcgaggcag taaccgtcga ggtcgattgt
      781 ccggcggtac agacgtcgcg aatctgctgc agccggccgg catgttcctg gcgcagcgcc
      841 tcgatcctgg catgcaggga catgaacggg cgtccgtgcg ccgggaggat caacgtgtcc
      901 tcgggcagtc gggcgagacg ctccagcgac accagatagt cgcccagcgg atcgccctgc
      961 tcgttggacg ggtacaggct aacgttgctc gagatcgtcg gcagcagctg gtcgccgctg
     1021 atcagcaccc gtgaggcatc gtcgtggagg cagacgtgtc cgggcgcgtg gccctcgtgg
     1081 acgctggtgc gccacaggcc gtcggtgcct gtcagcgtcg cctcggctcg caaggtggtg
     1141 atctgggggc gtccgctgac gatggcccgg tagcttccgc cacggaggat cgactgcatt
     1201 gccgcatcca gttcgatgcc gaggcgctcc atgaacccgt ccgagggata aacgggcccg
     1261 gcctccggcg cctccatgcc cgacacggct gcggcggcgg ccatgtcatg catggtgacc
     1321 ccgacttcga acctctcgga gagccaccgc gccatgccga agtggtcggg atggtggtgc
     1381 gtcaccagga cgaattcgag ttcgcgggcc agcggcaggc ggccctcgag ggactcccag
     1441 gcctcgcgca cgacccgcgt cttcattccc gtatcgacga ggaaccaccc cttgttgccc
     1501 gggacgagcc agacattgat gtgctgcagg gcgccgggga tcggaagccg gatccagagg
     1561 atgccgccca gaagctccag gtagccgcca ggctcggggg cgtccccgcg tgccagctcg
     1621 agcgttcgtg cgcgttgttc ggggctcatc gcggccgtgg gcaggctcca gagagggacc
     1681 gccacgatac acgagcgctt gtcagcgtcc agagagagcc cgcggatcgc ccggcggccc
     1741 gcatctgcca cggcgagagc cttccggcga cccgatgccc gcgatggaat cgaaggcagc
     1801 agccgtcagg aaccgttgcg cgaaaatttc gctcccccaa accctggtcg ttcaccgggc
     1861 gtgcggctgc gccaggccgg catcagtcgt cgaatatcga caactggctc tcctgcttcg
     1921 tcttgtcctt cgttccgcgc ttcgcccgct gccgcgcctg gcgcctggac ggcgtttcgt
     1981 tcgcgacggc tttggcaggg cgcccgggca atgcagagat gaaggtggcc aggtcggcaa
     2041 agctctccgg cttcacgctg taccggatga agcgaccgtc gggcgtggcc accaggagcc
     2101 cggcctgact gagttccttg agatgaaatg acagcgtcga tgcggggcag tggaccgcgc
     2161 gggcgatgcg ccccgcggca gtgccctccg gccccccctg tgccacgacc tcgacgatcc
     2221 ggagacgcgt cggctgggag agcgcagcga gtctgctttc gaaaagagcg ggtgcttcgg
     2281 ccatgaagtt ctccgccgga ggcaacggat gaggtttcta ggataatcga attcaacgcc
     2341 atctgggcgc atttattcgt acatcccacg accctgacat attgcatatt tgagagatcg
     2401 agctatattc aacccttaga gcattgagtc tgaacggaat ctcccccgtg ctcgcctggt
     2461 ggtctgcagc atgcggtcct gttacccgcc tgtcggggcc ccagagtcaa cacatgtaaa
     2521 acaacgattt atggagaggg gaatgaacgt cgcctgggag tcgtttcatc gagccacaaa
     2581 cgagctcgct tcctcagcat ctattaaaca tagacttacg gctgctttct cgaatcattt
     2641 gaaagatctg gagcccgaca gcctcccccc cgaactccgt ccacgcttcg aggaactgca
     2701 cgcaagcctt agccgtgtca ggccgctgcg cggcgagacg gccgtctggg cgaccgttcg
     2761 taaaatgtcg aacgatgaag ctgaaatcgt cgcccgccgc attgtggaac tgcttgggga
     2821 actggcccgt caccgggagc agctcccgaa gaccagcggc cgcccggttc tctccctcta
     2881 cgctgcggac ggctgaagcg ggtcagtcga gcttgaactc gatcgtatcc cagcgcgtgg
     2941 tttcctcgtg gcgcgcctgc cgacggatgg catcggccat ttccctcgtg ttccacgaga
     3001 caacttcgtc cgccacctcc cgcagctttg caggaacggt ttcaccggtc ccgaaaggtt
     3061 ccatgacgat gatgggtttc tcgcaggcct gggcgtacag cgcctgaaac tcgatcaatg
     3121 acgggtcacg ggcatgcaag gtggtcagga tcaggatcac ctc
//
LOCUS       DBNHOX010000212         3128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_596659, whole genome shotgun sequence.
ACCESSION   DBNHOX010000212 DBNHOX010000000
VERSION     DBNHOX010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_596659"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1227)
                     /locus_tag="AC16UZ_04680"
     CDS             complement(130..1227)
                     /locus_tag="AC16UZ_04680"
                     /inference="COORDINATES: protein motif:HMM:NF053593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3169 family protein"
                     /protein_id="HIR6246009.1"
                     /translation="MLTKADDYPIHQTPEPIAYSGTDRNFYDRYFFNGYQREGELFFA
                     AALGVYPHLNVMDAAFSILVGGRQYNVQASKVLHMERLNTQVGPIEVEVIEPLNVLRV
                     SCADPANGVAADVCFRARVPAVEEPRFTRRQGSLTFMDYTRLTQNGSYEGWLEVDGQR
                     YELTHDETWGTRDRSWGIRSVGARDPQPNPYAGEPQFYWLWAPLNFPSTNNYYHLNAD
                     REGRPWNTMGRLADPERSEEMAAAASSIRHRSGTRLAESATLEYVRENGEEATIELQP
                     TQTFYMRGLGYMHPEWGHGLFHGELATNYEVYETAEIADADPGYFHVQAICDAQLTDA
                     RGSERGKGVLEQLVIGAYEPYGFRELFDVAP"
     gene            1264..1599
                     /locus_tag="AC16UZ_04685"
     CDS             1264..1599
                     /locus_tag="AC16UZ_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246010.1"
                     /translation="MDWERYKQLCDQPNVFSRWMLNQTIELIGESPVADTLAGVLGSF
                     PLEKPDDHRGGPATDMFVLELESGVRRAVVELVLLAREAGAATEATSHRGLGGFVEAW
                     REYLNWTRG"
     gene            1678..2064
                     /locus_tag="AC16UZ_04690"
     CDS             1678..2064
                     /locus_tag="AC16UZ_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HIR6246011.1"
                     /translation="MSDDKAVVLELIDGFNSIDLDRIVACFTEDAVYDNIPMQAVQGT
                     EAIRASLEGFMGAASEVQWDLLNVVGEGGVVLTERIDKFKVNGTWIELPVMGTFEVAD
                     GKIKAWRDYFDLAQFQTQFAKATGAQ"
     gene            2081..>3128
                     /locus_tag="AC16UZ_04695"
     CDS             2081..>3128
                     /locus_tag="AC16UZ_04695"
                     /inference="COORDINATES: protein motif:HMM:NF014805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6246012.1"
                     /translation="MFRRLLIANRGAIARRVVRACNDLGIESVAVYSEADAHAPYLSE
                     ASRAVGLPGVSAGETYLNTAALLEAVRASGADAVHPGYGFLAENADFARAVAAAGATF
                     VGPDPKWLEVMGDKVAARRLLADHGFPVFPGSGGLSSLDEALSAAAELSYPVLLKPAA
                     GGGGIGMRLVRDEAELARAFASAQRLAEQAFGQGGIYLERAIDKPRHVEFQILADGAG
                     GALHLYERECSIQRRHQKIIEEAPAVGLPREELDALAARAEAVMAEIGYDNVGTLETL
                     CTQQGEFGFLEMNARIQVEHAVTEAVTGLDLVGLQIRLAAGAALPERPGLCGHAVEAR
                     IYAEDPRTLLPDTGV"
BASE COUNT          519 a          965 c         1097 g          547 t
ORIGIN      
        1 ggctctcccc acccgcaaat cccggtatgc cgcggcgacg ccgcccacgg accgggccgc
       61 cggcaagctt accgcatcgt gccaatcgtg ggtaaagggc cagggcgctt ggcagggggc
      121 agccgcaggt cacggcgcca cgtcgaagag ctcgcggaag ccgtagggtt cgtaggcgcc
      181 gatgacgagt tgctccagca cgcctttgcc gcgctccgag ccgcgggcat ccgtgagctg
      241 cgcatcgcag attgcctgga cgtgaaaata gccgggatct gcatcggcta tctcggcggt
      301 ctcgtagacc tcgtaattcg tcgccagctc gccgtggaac agcccgtggc cccactccgg
      361 atgcatgtag cccagcccgc gcatgtagaa ggtctgggtc ggctgcagct caatggtcgc
      421 ctcctcgccg ttttcccgca cgtactcgag ggtggcgctc tcggccagcc gggtgccgga
      481 ccggtgccgg atgctgctcg ctgcggccgc catctcctcg ctgcgctccg gatcggccag
      541 gcggcccatg gtgttccagg gtcgaccttc gcgatccgcg ttgaggtggt aatagttgtt
      601 ggtgctcggg aagttcaggg gcgcccacag ccagtagaac tggggctcac ccgcgtacgg
      661 gttgggctgg gggtcgcgtg cgcccaccga gcggatgccc caggaccggt cccgggttcc
      721 ccaggtctcg tcgtgggtga gctcgtaacg ctggccgtcg acctcgagcc agccctcata
      781 gctgccgttc tgggtgagcc gggtgtagtc catgaaggtg aggctaccct gacggcgggt
      841 gaagcgcggt tcttccacgg cggggacccg ggcacgaaaa cagacgtccg ccgccacgcc
      901 gttggcggga tccgcgcagc tcaccctcag gacgttgagc ggctcgatga cctcgacctc
      961 gatgggaccc acctgggtgt tcagccgctc catgtggagc actttggacg cctgcacgtt
     1021 gtattgcctg ccgccgacca gaatgctgaa ggcggcatcc atgacgttca ggtgcggata
     1081 gacgccaagc gccgcagcga agaacagctc accctcgcgc tggtacccgt tgaagaaata
     1141 gcgatcgtag aagttgcgat ccgtcccgga ataagcgatg ggctccgggg tctggtggat
     1201 gggatagtcg tccgcttttg ttaacatcga cctcttccag gggggcggaa aatcaggtta
     1261 ctcgtggact gggagcgcta caagcagctg tgcgatcagc ccaacgtctt ttcccgatgg
     1321 atgctcaacc agaccatcga gctgatcgga gagagtccgg tcgccgatac cctggcaggg
     1381 gtgttgggct cgtttcccct ggagaagccg gacgatcacc gcggcggacc cgcgacggac
     1441 atgttcgttc tggaactcga atcaggtgtc cggcgtgccg tggtggagct ggtcctcctg
     1501 gctcgggaag cgggcgccgc aaccgaagcg acctcgcatc gcgggttggg cggtttcgtc
     1561 gaggcctgga gggagtatct gaactggacg cggggctgac cgccagttgt gctgcctcgc
     1621 gcccgacggt gcgccgctcc ggcgagatcg gtaaaagaga taaagaggga gaacgacgtg
     1681 agtgatgaca aggcagtagt gctcgagctg atcgatggtt tcaacagcat tgatctggac
     1741 cgcatcgtgg cgtgcttcac cgaagatgct gtgtacgaca acattcccat gcaggccgtg
     1801 cagggcaccg aggcaattcg ggcgtcgctg gaggggttca tgggcgcggc cagcgaggtt
     1861 cagtgggacc tgctcaacgt ggtcggtgag ggtggcgtgg tgctcaccga gcggatcgac
     1921 aaattcaagg tcaacggcac ctggatcgag ctcccggtga tgggcacctt cgaagtcgcc
     1981 gacggcaaga tcaaagcctg gcgcgactac ttcgatctgg ctcagttcca gacccagttc
     2041 gccaaggcga cgggcgctca gtagccggac gagccgcgcc gtgttccggc gattgctgat
     2101 tgccaaccgg ggggccatcg cccggcgggt ggtgcgtgcc tgcaacgatc tggggatcga
     2161 gagcgttgcc gtttactccg aagcggacgc ccatgcgccg tacctgtccg aggcttctcg
     2221 ggcggtgggc ctgccagggg tgagcgccgg ggaaacgtac ctcaacacgg ccgccctcct
     2281 ggaagccgtg cgcgcaagcg gcgcggacgc cgtgcatccc gggtacggct ttctggcgga
     2341 gaatgctgac tttgcccgcg cggtggcggc tgcgggagcc acgttcgtcg ggccggatcc
     2401 taagtggctc gaggtgatgg gagacaaggt ggcggcccgt cgcctgctcg ccgaccacgg
     2461 ttttcccgtc ttcccgggca gtggcggtct cagcagtctc gacgaggctc tgagcgccgc
     2521 cgccgagctg tcataccccg tgctgctcaa accggccgcc ggaggcggcg gcatcggcat
     2581 gcggctggtt cgcgacgagg cggagctggc tagggctttc gcatctgcac aacggctggc
     2641 cgagcaggcg ttcgggcagg gaggcatcta cctcgagcgg gccatcgaca agccgcgtca
     2701 cgtggagttt cagattctcg ccgatggtgc tggtggggcg ttgcacctct acgaacgcga
     2761 atgttccatt caacggcgac accagaagat catcgaggaa gcgcccgccg tcggcctgcc
     2821 gcgcgaggag ctggatgcgc tcgccgcgcg cgcggaagcg gtgatggcag agatcggcta
     2881 cgacaacgtc gggaccctgg agacgctgtg cacgcagcag ggcgagttcg gcttcctcga
     2941 gatgaacgca cgcatccagg tggagcatgc ggtgaccgag gcggttacgg ggctcgatct
     3001 ggtggggctg cagatccgcc tggccgccgg cgcagcgctt cccgagcgcc ccgggctctg
     3061 cggccatgcc gtcgaagcga gaatttacgc ggaagatccg cgcacgctcc tgccggacac
     3121 gggcgttc
//
LOCUS       DBNHOX010000213         1879 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1560153, whole genome shotgun sequence.
ACCESSION   DBNHOX010000213 DBNHOX010000000
VERSION     DBNHOX010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1560153"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="AC16UZ_04700"
     CDS             complement(<1..461)
                     /locus_tag="AC16UZ_04700"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6246013.1"
                     /translation="MDIHVVPVDWSTHQHQLNEVRDAVFVREQGVPQELEWDGLDADA
                     THFLAVNEAGQALGCARLLGTGQIGRMAVVASQRRGGIGRRLLEAAVEKACEDGHAEV
                     FVHAQTEVARFYREAGFVPEGQEFMEADIPHIAMRLILPVEFRPSAEPIPAP"
     gene            complement(461..1831)
                     /gene="purB"
                     /locus_tag="AC16UZ_04705"
     CDS             complement(461..1831)
                     /gene="purB"
                     /locus_tag="AC16UZ_04705"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009683124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR6246014.1"
                     /translation="MQTHPLMTIGALDGRYRNRLEPLAPLVSEFGLMRYRVLVECRWF
                     AHLAQTDSIGELPALSSGQAAALAAVAEDFSLADAERIKAIERTTNHDVKAVEYFVKE
                     RVAAIDGLASHIEFVHFACTSEDVNNLSYALMLRDSRELLGEQMQALIDQISAVADDA
                     AEIPMLSRTHGQAASPTTMGKELKNVAYRLSRQLRQLREVALLGKMNGAVGNYNAHVV
                     AYPEVDWPELARAFVEGLGLAQNPLTTQIEPHDYIAELFHALVRFNQILLDFDRDMWG
                     YIAIEYFVQQPVAGETGSSTMPHKVNPIDFENSEGNLGIANALLEHMAAKLPVSRWQR
                     DLSDSTVLRNVGSACGHCSVAYQASQRGLGRVKINADRIRADLDASWEVLAEAVQTVM
                     RKHGLIEPYEQLKAATRGKRIDRDLYSEMLETLDLPASAREELRALTPASYTGLAADL
                     TKREPS"
BASE COUNT          300 a          583 c          644 g          352 t
ORIGIN      
        1 ggagcgggaa tcggctcggc gcttggcctg aactccacgg gcaggatcaa acgcatggcg
       61 atgtgcggta tgtccgcttc catgaattcc tgcccttcgg gcacgaaccc ggcttcgcgg
      121 tagaagcggg caacctcggt ctgggcgtga acgaagacct cggcgtggcc gtcctcgcac
      181 gctttctcca cggccgcctc caggagcctt cggccgatcc cgccgcgacg ctgggacgcc
      241 accaccgcca ttcgcccgat ctggccggta ccgaggagcc tggcgcagcc cagcgcctgg
      301 cccgcttcgt tgacggcgag aaagtgcgtg gcgtctgcat cgagaccgtc ccactccagt
      361 tcctggggca ccccctgttc cctgacgaag acggcgtcgc ggacctcgtt gagctggtgc
      421 tggtgggtcg accaatccac gggcacgacg tgaatgtcca tcagctgggt tctctcttgg
      481 tgaggtcggc ggccaggccg gtgtaggaag ccggcgtcag cgcgcgcagt tcttccctgg
      541 cgctcgcggg cagatcgagg gtctcgagca tctcgctgta cagatcgcga tcgatccgtt
      601 tgccgcgggt ggcggctttc agctgctcgt agggctcgat caggccgtgc ttgcgcatga
      661 cggtctgtac ggcctccgcc agaacttccc agctggcgtc caggtcggcc cggatccggt
      721 cagcgtttat cttcacgcgg ccgaggccgc gctggctcgc ctggtaggcg acgctgcagt
      781 ggccgcaggc agaaccgacg ttgcgaagca cggttgaatc cgacaggtcc cgttgccagc
      841 gcgagacggg cagcttggcg gccatgtgct cgagcagggc gttggcgatg cccaggttgc
      901 cctcggagtt ctcgaagtcg atgggattga ccttgtgggg catggtggac gagccggttt
      961 cgccggcgac cggctgctgg acgaagtact cgatggcgat gtagccccac atgtcgcggt
     1021 cgaagtcgag cagtatctga ttgaatctga ccagcgcgtg aaagagctcg gcgatgtagt
     1081 cgtggggttc gatctgggta gtcagcgggt tctgcgcgag gcccaggcct tcaacgaaag
     1141 ccctcgccag ctcgggccag tcgacttcgg gataggccac cacgtgagcg ttgtagttgc
     1201 cgaccgcccc gttcatcttg cccagcaggg ccacctcacg cagctgcctg agctgacggg
     1261 acaggcggta agcgacgttt ttgagctcct tgcccatggt ggtgggcgag gccgcctgac
     1321 cgtgggttcg ggacagcatc gggatctcgg ccgcgtcgtc ggcgaccgcg ctgatctgat
     1381 cgatgagcgc ctgcatctgc tcgccgagca gctcgcgaga gtcgcgcagc atcagggcgt
     1441 aagagagatt gttgacgtcc tccgacgtgc aggcgaagtg gacgaactct atgtgcgagg
     1501 cgagcccgtc gatagccgcg acgcgctcct tgacgaagta ctccacggcc ttcacgtcgt
     1561 gattcgtggt ccgctcgatc gccttgatgc gttcggcgtc cgcgagcgaa aagtcttccg
     1621 ccacggcggc aagggcagcg gcctggccgg aagatagggc gggcagctcg ccgattgaat
     1681 cggtctgggc aagatgagcg aaccagcggc actccaccag cacgcggtag cgcatcagtc
     1741 cgaactcgct taccagcggt gccagtggtt cgaggcggtt ccggtagcgc ccgtccaggg
     1801 cgccgatggt catgagcggg tgtgtctgca cggaggtggt cacgttttcc tctcgagagt
     1861 ttccaaggct accagcagc
//
LOCUS       DBNHOX010000214         4595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_16930, whole genome shotgun sequence.
ACCESSION   DBNHOX010000214 DBNHOX010000000
VERSION     DBNHOX010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_16930"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="AC16UZ_04710"
     CDS             <1..157
                     /locus_tag="AC16UZ_04710"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HIR6246015.1"
                     /translation="VTEFFRQPDAHLQFEALEWLTMGQRLSIPMVIIGILLLFWPRLR
                     GGGETRG"
     gene            264..1058
                     /locus_tag="AC16UZ_04715"
     CDS             264..1058
                     /locus_tag="AC16UZ_04715"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR6246016.1"
                     /translation="MKQYLDLMRQVRDTGVFKDDRTGTGTYSIFGHQMRFDLSESFPL
                     VTTKKVVLRLIIHELLWFLQGSTNIKYLTDHNVHIWDEWADEEGNLGPVYGSQWRSWP
                     TPDGGTIDQIAAVVQSLRTNPNSRRHIVSAWNVAEVDSMALPPCHTMFQFYVAQGRLS
                     CQLYQRSADIFLGVPFNIASYALLTMMIAQVCDLQPGEFIHTFGDAHLYANHLEQADE
                     QLSRVPLPLPTVTLNPEVRDIFGFRYEDFTLTNYRCHGTIKAPIAV"
     gene            1065..1568
                     /locus_tag="AC16UZ_04720"
     CDS             1065..1568
                     /locus_tag="AC16UZ_04720"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR6246017.1"
                     /translation="MHVALIWAMARNRVIGRDNRLPWRLPRDMQFFVATTMGKPVIMG
                     RRTFESMKAPLAGRTNIVVTSDTGYRREGVLVAHSLEAALDLAQAQCRADDVEEAMVI
                     GGAEVYRVALPIATRLYVTLIDADVEGDTFFPEIDLDAWREVSREAFPADAGNGLPFS
                     IVVFERP"
     gene            1581..3560
                     /locus_tag="AC16UZ_04725"
     CDS             1581..3560
                     /locus_tag="AC16UZ_04725"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR6246018.1"
                     /translation="MTPRRIKGLCSPLGWILLVLRAGCARAPAPVPEPEAGRPGNVVV
                     SGVPAIPAGAAHGLMRYRNVRSAYLMGWADDGLVIATRFGETTQLHRLKTPLGAREQI
                     TFFSEPVLAAFLPTGAGALGAPGFVLSRDAGGSEHYQLFLFDWSTRHARMVSNGRARY
                     TNVLWSNRGDQYAYTTTERDGRRWDIHLQDLAGGIKVALETSDGGWRAVDWSPGDDRL
                     LLIKYVSATESYLYELELASGSLRPLLDESLAVAIGGARYAGNGESVYFTADLGAEFM
                     RLHLLELATNSVEVLTADVPWDVTEIAVTSDGAHLAMVTNEGGVSRLGVLRLPDHSPL
                     ALPEVPAGTIYGLRLSPDGRQLGFTVNAPTAPGDVYSIDLVGRTLTRWTRSEVGGLDP
                     SAFAAPSLVHYPTFDREGGARRKIPAFVYMPDGPGPHPVVIHLHGGPHSQYRPKFSAS
                     AQYYVKELGLAVVAPNFRGSSGYGKNYLKLDDGYLREDAVRDVGALLDWIAAQPRLDA
                     DRVAVSGGSYGGYMALASLIRYGDRLVAGVSRVGISNFVTFLENTRAYRRDLRRVEYG
                     DERDPQMRAFLEGISPLGQADRIDSPLLVIHGFNDPRVPASESEQIADAVSAAGTPVW
                     YVLAMDEGHGFRKKANSDYAAAVTALFLQRYLLDD"
     gene            3553..4593
                     /locus_tag="AC16UZ_04730"
     CDS             3553..4593
                     /locus_tag="AC16UZ_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013663.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR6246019.1"
                     /translation="MTEGPGSRAGVAICVNPMSGRDVRRLAARASNMTHEAKRDMVAR
                     VAAGAQAMGASDIYVTREPFRIASLALEHMGLAAAGHVLQTPLTNGPADTEAAVAAFL
                     AAGCRVIVSLGGDGTNRAIARALGDAGAAGAGVDLIPLSTGTNNVFPVLAEPTAAGMV
                     AGLIASGRLPDDDLRQTAKVIHLSGTDARGRAVRDLALVDAALLRDDHVGNLLPFAAQ
                     RLARIVLTRAEPDAVGMSPIGGMLDVVEAGDDEGLLVEMGPGREFSAPLSPGAFQAVS
                     VRSASRLPLDVTVPLAGPGVIALDGDRDYKIAPREALTARIRRDGPRVVDIAAAMRRA
                     VARGIMAPARNG"
BASE COUNT          733 a         1483 c         1546 g          833 t
ORIGIN      
        1 cgtcaccgag ttcttccgcc agccggatgc ccacctccag ttcgaagcgc tggagtggct
       61 gaccatgggc cagcggctct cgataccgat ggtcatcatt ggtattctgc ttctgttctg
      121 gccccgcttg cgtggagggg gcgaaacgcg gggctgacaa gcgagcgcca acagccggca
      181 ctggccccga tcgccacagc gggtccggta atcacggggc cgcgagcgcg gccgaactcg
      241 ggacgagcag ccaacggaag ccagtgaaac agtatctcga tttgatgcgc caagtgcgcg
      301 atacgggcgt cttcaaggac gaccgcaccg ggacgggaac gtacagcatc ttcggtcacc
      361 agatgcgctt cgatctgagc gagagctttc cgctggtgac gacgaagaag gtggtgctca
      421 ggctgatcat ccacgagctg ctctggtttc ttcagggcag cacaaacatc aagtacctca
      481 ccgaccacaa cgtgcacatc tgggacgagt gggcggacga agagggcaac ctgggacccg
      541 tctacggatc tcaatggcgt tcctggccga cgccggacgg aggcaccatc gatcagattg
      601 ccgccgtcgt gcagtctctt cgcaccaacc ccaattctcg gcgccacatc gtgagtgcct
      661 ggaacgtcgc ggaggtggac agcatggcgc tgccgccctg ccacaccatg tttcagttct
      721 acgtggccca gggccggctt tcctgccagc tttaccagcg cagcgccgac atcttcctgg
      781 gtgttccctt caacatcgcc tcctatgcgc tgctcaccat gatgatcgcc caggtctgtg
      841 atctgcagcc cggcgagttc attcatacct tcggtgacgc gcatctctac gccaatcatc
      901 tcgagcaggc ggacgagcag ctctcgcgtg ttccgctgcc gctgcccacg gtcacgctga
      961 atccggaagt gcgggacatt ttcggtttcc gctacgagga cttcaccctc acgaactacc
     1021 gctgtcacgg caccatcaaa gcgccgattg cggtctgacg cgcggtgcac gtcgcgctga
     1081 tctgggccat ggcgcgcaac cgcgtgatcg gccgtgacaa ccggcttcca tggcgcctgc
     1141 cccgggacat gcagtttttc gtggccacga caatgggcaa accggtgatc atgggtcggc
     1201 ggacgttcga gtccatgaag gcgccgctgg ccgggcgcac caacatcgtc gtcaccagcg
     1261 ataccggtta tcggcgcgag ggagtgctgg ttgcccacag cctggaggcc gccctcgacc
     1321 tggcgcaggc ccagtgccgg gcggatgacg tcgaagaggc catggtcatc ggcggcgcgg
     1381 aggtctatcg agtggcgctg ccgattgcta cccggctcta cgtaacgttg atcgatgcgg
     1441 acgtggaagg cgacaccttc tttccggaga tcgacctgga cgcatggcgc gaggtgagcc
     1501 gggaagcgtt tcccgcggac gccggcaacg ggctgccgtt ttccatcgtc gtcttcgagc
     1561 ggccctaggt ggcgggccgg gtgacgccgc gtcgtatcaa gggcctttgc tcgccgttgg
     1621 gctggattct gctcgtcctg agagcggggt gcgcgcgcgc cccagcgccg gttccggaac
     1681 ctgaggctgg gcggcccggc aacgtggtgg tgtcgggcgt tccggccata cccgcgggcg
     1741 cggcacatgg gctcatgcgt taccggaacg tccgatctgc ctacctcatg ggctgggccg
     1801 atgacggact ggtcatcgcc acccgcttcg gcgaaaccac tcagctgcat cggctgaaga
     1861 cgccgctggg cgcgcgggag cagattacgt tcttctcgga accggtcctg gcggcctttc
     1921 ttcccacggg cgctggcgct ttgggcgctc cgggctttgt cttgagtcgc gacgcggggg
     1981 gctcagagca ctatcagctg tttctcttcg actggtctac gcgtcatgcg cgcatggtta
     2041 gcaacggccg ggcccgctac accaacgtgc tctggtccaa ccgaggcgac cagtatgctt
     2101 acaccaccac ggagcgggac ggccgccgct gggacatcca cctccaggat cttgccgggg
     2161 gaataaaggt cgcgctggaa acctctgatg gaggctggcg agcggtagac tggtcccccg
     2221 gggacgatcg cctgctgctc atcaagtacg tttcggcgac cgagtcttat ctgtacgagc
     2281 tcgaactcgc atcgggcagc ttgagaccgc tgctggacga aagccttgcc gtggccattg
     2341 gcggcgccag gtacgcgggg aacggcgaaa gcgtctactt cacggccgac ctgggcgccg
     2401 agttcatgcg tctgcatctt ctcgagctcg cgaccaacag tgtcgaggtg ctgactgctg
     2461 acgtaccctg ggacgtcacg gagatcgccg ttacctcgga tggggcccac ctggccatgg
     2521 tgaccaacga gggcggggtc tcgcggctgg gggttttgcg gttgcccgac cactcgcccc
     2581 tggcgctgcc ggaagtgccc gcgggaacca tctacgggct gcgattgtcg cccgacggcc
     2641 ggcagctggg cttcacggtc aatgcgccca ccgcccccgg cgacgtctac agcattgacc
     2701 tcgttgggcg aacgcttacc cgatggaccc gaagcgaggt gggcggtttg gatccatcgg
     2761 cctttgccgc gccgagcctg gtgcactacc ccaccttcga tcgggaagga ggcgcgcggc
     2821 ggaagattcc cgccttcgtc tacatgcccg acgggccggg gccccatccg gtcgtcatcc
     2881 acctgcatgg tggtccgcac agccagtacc ggccgaagtt ttccgcatcg gcccagtact
     2941 acgtaaagga gcttggcctg gcggtggttg cgcccaattt ccgggggtcg tcgggttacg
     3001 ggaagaacta cctcaagctg gacgacggtt atcttcgcga ggacgcggtc cgcgacgtag
     3061 gtgctctgct ggactggatc gcggcccagc cgcgtctgga tgcggaccgc gtcgccgtca
     3121 gcggcggctc ctacgggggc tacatggcac tggcctccct gattcgatac ggtgaccggc
     3181 tggtcgccgg cgtctcccgg gtgggcatca gcaacttcgt cacgttcctg gagaacacgc
     3241 gcgcctaccg tcgcgacctt cggcgggtgg agtacggaga cgagcgggat ccgcagatgc
     3301 gcgcgtttct cgaagggatc tctccgctcg gccaggctga ccggatcgac agccccctgc
     3361 tcgtgatcca cggattcaac gacccgcgag tgcccgcatc ggagagcgag cagatcgccg
     3421 acgccgtgag cgccgcgggt acgccggtct ggtatgtcct cgccatggac gaagggcacg
     3481 gattccgcaa gaaggccaac agcgattacg cggccgcggt tacggccctt ttcctgcagc
     3541 gctacctgct cgatgactga agggccgggc tcacgagctg gggtggccat ctgcgtcaat
     3601 cccatgtccg gtcgagacgt caggcgtctg gccgcgcgcg caagcaacat gactcacgag
     3661 gccaaacgcg acatggtggc gcgggtggca gccggcgccc aggcgatggg cgccagtgac
     3721 atctacgtga cccgggagcc gtttcgaatc gcgtcgttgg ctttggagca catggggctc
     3781 gccgcggccg gacacgtgct tcagacgccg ctgaccaatg gacctgccga caccgaagcg
     3841 gcggtggcgg cttttcttgc cgccggctgc cgggtaatcg tctcgctggg tggggacggg
     3901 acgaaccggg ccatcgcccg cgcccttggg gatgcgggcg cagcgggtgc gggcgttgac
     3961 ctcataccgc tttccacggg aaccaacaac gtctttcccg ttctggcgga gcccactgcc
     4021 gcgggtatgg tggcggggct gatagcctct gggcggctgc ccgacgacga tctccggcag
     4081 acggccaagg taattcattt gtcgggcacc gatgcccggg gacgagccgt tcgggacctt
     4141 gcgctggtgg acgcggcgct gctgcgtgat gaccacgtgg gcaacctcct gccgttcgcc
     4201 gcgcagcggc tagctcgaat cgtgctgacc cgggccgagc cggatgccgt gggcatgtcg
     4261 cccatcgggg ggatgctcga tgtggtcgag gcgggggacg acgaaggcct cctggtggag
     4321 atgggtccgg gcagggagtt ctcagcgccg ctctcgcccg gcgctttcca ggcggtttcg
     4381 gttcgctcgg cctcgcgtct gccccttgat gtgacggtcc ccctggcagg gcccggtgtg
     4441 atcgcgctgg acggtgaccg ggactacaag attgcccccc gggaggcgct cacggcccgt
     4501 atccgtcggg atggccccag ggtcgtcgac atagccgcgg caatgcgtcg tgccgtcgct
     4561 cggggtatca tggcgcccgc ccgcaacggt tagac
//
LOCUS       DBNHOX010000215         2426 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1046796, whole genome shotgun sequence.
ACCESSION   DBNHOX010000215 DBNHOX010000000
VERSION     DBNHOX010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1046796"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..325)
                     /locus_tag="AC16UZ_04735"
     CDS             complement(5..325)
                     /locus_tag="AC16UZ_04735"
                     /inference="COORDINATES: protein motif:HMM:NF020317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR6246020.1"
                     /translation="MAKYLVQANYVGDGVAGLMKEGGSKRRAAVEKLFGAVGGKVEAF
                     YYAFGDTDLFVIGDLPDNASAAALSLAVNATGAATCSVRVLLSAEEMDAAAKKAPGYR
                     APGR"
     gene            complement(395..538)
                     /locus_tag="AC16UZ_04740"
     CDS             complement(395..538)
                     /locus_tag="AC16UZ_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246021.1"
                     /translation="MEPCSGRIDLAIGQWLAFMCIAPASADVQGDGQELVQGPIVDAK
                     AIK"
     gene            complement(922..1221)
                     /locus_tag="AC16UZ_04745"
     CDS             complement(922..1221)
                     /locus_tag="AC16UZ_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246022.1"
                     /translation="MFRFERTAAVKHAAEVPAAVKFAAEVTSYLNKRHDLNMKFGVEL
                     FGSPRIHWYYDLDSLDESAKLDATLLKDSDYEQMLKKNEDLWVDQSMHDTIVRLS"
     gene            complement(1711..2274)
                     /locus_tag="AC16UZ_04750"
     CDS             complement(1711..2274)
                     /locus_tag="AC16UZ_04750"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019485088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6246023.1"
                     /translation="MATRDLLIRDESEQDADAISEVTVAAFESLEVSSHTEQFIVDAL
                     RSARALTVSLVAEMDGRIVGHVAFSPVTIPGGVGGWYGLGPVSVLPMYQRRGIGTALI
                     REGLSRLKGLGARGCCLVGHPQYYRRFGFENIPGLVSPGVPAEVFFALSFDGHFPQGS
                     VAFHEAFGATGPTALAEYTPPAGADQP"
BASE COUNT          449 a          818 c          730 g          429 t
ORIGIN      
        1 tcgtctagcg tccgggcgct cgataaccgg gcgccttctt ggccgcagcg tccatctcct
       61 cagcgctcag cagaaccctg acgctgcagg tggcagcccc ggtggcgttg accgccagcg
      121 acagtgccgc cgcgctcgcg ttatcaggca agtcaccgat cacgaacagg tcggtgtcac
      181 caaaggcgta atagaaagcc tccaccttgc caccgaccgc tccaaacagt ttctcgaccg
      241 ctgcgcggcg tttggagccg ccttccttca tcaaccccgc gacaccgtcc ccgacatagt
      301 tggcttgaac gagatacttg gccatacata cccccgttag aaaaggacat ctccagccct
      361 tgcactgggc gctttgcacc cgttgcaggc gacttcactt gatggctttg gcatcgacaa
      421 tgggcccctg caccagttcc tgcccgtcgc cttgcacatc cgcgcttgct ggcgcgatgc
      481 acatgaatgc cagccactgc ccgatagcca gatcaatccg gccagagcag ggctccattt
      541 acgcccgatc gtcggcgaac ttgcaaggag gatccgttca tgctgcggtg cgttcgaaac
      601 gggccttcat ctggccccaa gccccgcgcg ccaagcccgc acggcagaag gtcgcacccg
      661 ccatccttca agcctttgca tcgatggtgc ctgcctgccc tgcaggcaca ggttcaccct
      721 tcgaaggccc gccagcacgg ccatgaccgc aacgagtggc gtcccggcca ggcagaaggt
      781 ccggatacgt cgttgtcgac gcaaggtgcg ccgcagtaat cgacggttac cgaacgtgag
      841 cggaccttgc ggtcgatggc ctgcaaacaa acgagcgcac cgtcggtgcg ctcgtcgctc
      901 ggagtcgtgc cagcttcctt gctagctgag tcgcacgatg gtgtcgtgca tgctctggtc
      961 aacccagaga tcttcgttct tcttcagcat ctgctcgtag tcgctgtcct tgagcagcgt
     1021 cgcatccagc tttgccgatt cgtcgaggct gtccaggtcg tagtaccagt gaatcctcgg
     1081 cgagccgaac aactcgacac cgaacttcat gttcaggtca tggcgcttgt tgaggtatga
     1141 ggtaacttcc gcagcaaatt tcaccgctgc aggaacctcg gctgcatgct tgacagcggc
     1201 cgtacgttcg aagcgaaaca tggtggcccc ctatatctgg tggagacttc aacgggccgg
     1261 cgaacagcga tacacctcgc ggcactgcag caagaaatgc tccgccatcc gccgggtgtt
     1321 tctctagtag attccgtcac gccggcgaaa acaatccgca gaagtggcaa agtacggccg
     1381 cgagcccatg cgcctggcgg gccgcgcggc gggactgcct tgactgcgtc caatgcccca
     1441 gcgcctggcc cgggcagctg gcgggctgcg ggcgtgacga gagccgtggc cccggcacca
     1501 tggccggcga caagtaggca agccccacgg caagaaaccg gagccaagcc accggcctgg
     1561 gcaaactcgg gcaaatgccg atgtgcaacg aaggccgccc acgtgggcgg ccttcttccc
     1621 tggatcacag ggccgcccgt ctccaacctg gcccggctgt gccggcgctc gtagacgctt
     1681 cggattccgg ctcccggccg tcagcggacc tcagggctgg tccgctcccg cgggaggcgt
     1741 gtattccgca agcgccgtcg ggcccgtcgc gccgaatgcc tcatggaacg cgacgctgcc
     1801 ctgtggaaag tgtccgtcga atgacagggc gaagaatact tctgctggaa ctcccgggga
     1861 gacgagcccg ggaatgttct cgaatccgaa tctccggtag tactgcggat gcccgacgag
     1921 gcagcaaccc ctggcgccca ggcccttcag tcgtgacagc ccttcccgga tcagggccgt
     1981 gccaatgccc ctgcgctggt acatcggcag caccgagacc gggccgagcc cgtaccagcc
     2041 gccaacgcca cccgggatgg tcacgggaga gaacgcgacg tgcccgacga tgcggccgtc
     2101 catttctgca acgagcgata cggtcaaggc cctcgcggag cgcagtgcat ccacgatgaa
     2161 ctgctcggtg tgactgctga cctccaggga ctcgaaggcc gcgaccgtca cctcggaaat
     2221 ggcatcggca tcctgttccg actcatccct gatcagaaga tcccgtgttg ccatggcgac
     2281 ctcgacttct ggtgttgcgc ccggcctggc caggcccagg gtgcccgctg gcgacccaag
     2341 gcgggcatgt tcggaatgca gttctcgatg gcggcgagcg gtccggggaa aaaccgcccg
     2401 gaagggcccg tcgcgtgtat ttcatg
//
LOCUS       DBNHOX010000216         5853 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_146611, whole genome shotgun sequence.
ACCESSION   DBNHOX010000216 DBNHOX010000000
VERSION     DBNHOX010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_146611"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="AC16UZ_04755"
     CDS             <1..615
                     /locus_tag="AC16UZ_04755"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6246024.1"
                     /translation="DTWHTSGLCGTGSHDIEVKDAEVPAGRWVTMGKRARVDAPLYRF
                     PTLGLLALGVSAVALGIAEHAIEAFTTLARDKVPTGSARPLSARSGAQRDLARAMAAV
                     QSAAALTREAIEDAWGEAHREGSLGLETKARLRLAATNNAWSAAEAVDLLYHAAGGSS
                     IYAHSALQRCFRDVHVATQHLMVAQPIYEVVGKVALGIDPKTLL"
     gene            730..1611
                     /locus_tag="AC16UZ_04760"
     CDS             730..1611
                     /locus_tag="AC16UZ_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR6246025.1"
                     /translation="MGHDHNHIHTRDNEVIVGRAFVLIAGFMVVEVIGGLLANSLTLL
                     ADAGHMFLDATALGLSWYALRLSKRSNDHKLSYGYHRFQVLAAFINGLTLMALVVWIL
                     VEAYARLRAPEGMLALPALAVAVVGMVVNLIAFRWLHGAEDNATVRSAALHVIGDLLG
                     SAAAIIAALTVYLTGWLYADPLLAVVIVVILGRGAWRVLKESAHILLEGVPHGINLTE
                     IAETLAARVPGVIEVHHVHAWALTSEKPLVTLHASVDEGTELAGVVSRMKVVLNDDFG
                     IDHSTIQVEHGPCPDDD"
     gene            1626..2009
                     /locus_tag="AC16UZ_04765"
     CDS             1626..2009
                     /locus_tag="AC16UZ_04765"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIR6246026.1"
                     /translation="MTKPGYTGIKRIRKAAGYSSQGIRAAWQHESAFRQECTVALVLL
                     PCAVLLAETWMQLAVLITVCVLVLIAELLNSAVEAVVDRIGHEHNDLAGQAKDMASAA
                     VALAIFLVPLIWGLVALERFVGYWI"
     gene            complement(2055..3602)
                     /locus_tag="AC16UZ_04770"
     CDS             complement(2055..3602)
                     /locus_tag="AC16UZ_04770"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0032787 - monocarboxylic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6246027.1"
                     /translation="MENNLGLFLTKRAFLTPQREGYVDSHSGLRLTFRQLNERSNQLA
                     NSLLQAGVQKEERVGLLLMNSAEFMEAYFALAKIGAVVVPLNWRLVADELEFILKDSG
                     TARLIFDEDFVDTVAELHARGDRTDIRHWLQVEGSQEVAHFAESYEAFRDNGAAEEPP
                     CTACDDDMLYIMYTSGTTGLPKGVVHTHTTAIWAILTIAANAYYEETDRYLGALPMFH
                     VGALTPLAVNVYRGATSVVMRAFDPVLAWELIDREKITSGLMVPAMLNFMLQVGEAKD
                     RYDYSALRWIMTGAAPVPVALTEQYVQMGISILQVYGLTESCGPACLMDSENALRKPD
                     STGKAFFHTDVRVVDAHGNDCAPGEAGEVVVRGKHIMREYWNRPDATTETIVDGWLHT
                     GDVAVMDEEGFVSIQDRIKDMIISGGENVYPAEIEGVLISHPKISEAAVIGQPSERWG
                     ESPLAIVVKADPDLDVAEVMEFCRGKLAGYKQPKGVEFVDEIPRNPSGKILKRILREQ
                     FPGPAPA"
     gene            4028..4930
                     /locus_tag="AC16UZ_04775"
     CDS             4028..4930
                     /locus_tag="AC16UZ_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6246028.1"
                     /translation="MVSRSAIDDDLYRIGTVATLTGISVERLRAWERRYGLAPTYRSG
                     KTRFYSKQQLQTLQRIKRLIDQGHPISSLAPLSDEQLDERLSAATPRLQALQSATVGL
                     IGPNLLVLEQQQGSDNRLEVRARWANMDAFMSDQTGTERIDVIVAQLPVLLVDHINNL
                     ARFHPESRIVAIYQFATPAQIAAVQELGIPTLTWPAGWQEIEHACATTAGLPLRAART
                     AVRRFSDAELIAISASEHGDPAACPQHLVELISGLNAFAEYTLNCADEAAEPEVYQRV
                     HTDTTQARAQLELALEMMVDQEAG"
     gene            5526..>5853
                     /locus_tag="AC16UZ_04780"
     CDS             5526..>5853
                     /locus_tag="AC16UZ_04780"
                     /inference="COORDINATES: protein motif:HMM:NF040138.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR6246029.1"
                     /translation="MPLSVDFDASGSSDADGRIASYAWTFGEPPGASSTSGVNTSHTY
                     EEAGTYTVELTVTDDDGATGSTTRTVTVEGPVASISGTVRILASSAIDSDVNDFGSTP
                     APNNDFA"
BASE COUNT         1074 a         1976 c         1774 g         1029 t
ORIGIN      
        1 gatacctggc acacgtcggg cttatgcggg accggcagcc acgacatcga ggtaaaggac
       61 gccgaggttc ccgcggggcg atgggtcacc atgggcaagc gcgcgcgggt ggacgcgccc
      121 ctctaccgat ttcctacgct gggcttgctc gcccttggcg tgtccgcggt tgcactgggc
      181 atcgcagagc acgccatcga ggctttcacc acgctcgccc gggacaaggt ccccaccggc
      241 agcgcccggc ctctttccgc ccggtctggg gcccagcgcg acctggccag agcgatggcc
      301 gccgtccagt cagcggcggc gctcacccgc gaggccatcg aggacgcctg gggggaagcc
      361 caccgcgaag gatccctcgg cctggaaacc aaggcccgcc tcaggctggc cgccaccaac
      421 aacgcctggt cggcggccga ggcggtggac ctcctctacc atgccgcggg aggatcgtcg
      481 atttacgcgc acagcgcgct gcagcggtgt tttcgcgacg tgcacgtcgc cacgcagcat
      541 ctgatggtag cgcagcctat ctacgaggtg gtcggcaagg tcgccctggg catcgatccg
      601 aagacgctgc tctgagccga gtcccgtggc gacgtgaccc acgacgcccg ggacggagcg
      661 gaccaccgag cgcggtgagt cggtataatc acgggcgttc gtgagtcggc tgcaggacaa
      721 cgcgtaaacg tgggccacga ccacaaccac attcatacca gggacaacga ggtcatcgtt
      781 ggccgcgctt tcgtgctcat cgccggcttc atggtcgtcg aggtcatcgg tgggctgctg
      841 gccaactccc tgaccctgct cgccgatgcg gggcacatgt tcctcgacgc caccgcgctg
      901 ggcctgtcct ggtacgcgct ccggttgtcg aaacgctcca acgatcacaa gctctcctac
      961 ggctaccacc gctttcaggt gctggccgca ttcatcaacg ggctcaccct gatggcgctc
     1021 gtggtgtgga tactcgttga ggcctacgcc cggctgcgcg cgcctgaagg aatgctggct
     1081 ctgcctgcgc tggccgttgc cgtcgtcggc atggtggtca acctgatcgc cttccgctgg
     1141 ctgcacgggg ccgaggacaa cgccaccgtg cgcagcgccg cgctccacgt catcggcgac
     1201 ctgctcggct cggcggccgc catcatagcc gcgctgaccg tctatctgac cggttggctc
     1261 tacgccgatc cgctgctcgc cgtcgtcatc gtcgtcatcc tcggccgggg cgcctggcgg
     1321 gtgctgaagg aatcggccca cattcttctg gaaggtgtgc cgcatggcat caacctcacg
     1381 gagatcgccg agaccctcgc tgcgcgggta cccggagtga tcgaggttca ccacgtgcac
     1441 gcgtgggcac tgacctccga gaagcccctg gtcacgctgc atgcaagcgt cgacgagggg
     1501 accgagctcg ccggggtggt gtctcgcatg aaggtcgtgc tcaacgacga tttcggcatc
     1561 gatcactcga ccattcaggt tgaacacggg ccttgcccgg acgatgatta ggctcgaggg
     1621 ctaggatgac caaaccaggc tacaccggga tcaagcggat cagaaaggca gcgggctact
     1681 cgtctcaggg catccgcgcc gcctggcaac acgagtccgc ctttcgccag gaatgcaccg
     1741 tggccctggt tctgctgccc tgcgccgtcc tgctggcgga gacgtggatg cagctggccg
     1801 tgctgatcac ggtctgcgtg ctggtgctga tcgcggagct gctgaactcg gcggttgaag
     1861 cggtggtgga caggataggt cacgagcaca acgatctggc cggccaggcc aaggacatgg
     1921 cttccgccgc cgtcgcgctg gcgatcttcc tggtgcctct gatatggggc ctcgtggccc
     1981 tcgaacgctt cgtcggctac tggatctgat ccgacccctg cggaggaccc gccacccgcg
     2041 gcctcgacag acggtcaagc aggcgcggga ccgggaaact gctcccgcag gatgcgcttg
     2101 aggatcttgc cgctgggatt gcgcggaatc tcgtcgacga actccacccc ttttggctgt
     2161 ttgtaccctg caagcttgcc gcgacagaac tccatcacct cggcaacgtc cagatcggga
     2221 tcagccttta ccacgatggc caggggagat tcgccccaac gctcgctcgg ctgaccgatc
     2281 acggccgcct cggagatttt cgggtggctg atcagcacgc cctcgatctc ggcggggtaa
     2341 acgttctccc cgcccgagat gatcatgtcc ttgatccgat cctggatgga aacgaacccc
     2401 tcttcatcca tgacggcaac atcgccggtg tgcagccagc cgtccacgat cgtctccgtg
     2461 gtggcgtccg gccggttcca gtactcacgc atgatgtgct tgccccgcac caccacttcg
     2521 ccggcctcgc ccggcgcgca gtcgttgccg tgggcgtcga ccacccgcac atccgtgtgg
     2581 aagaaggcct tacccgtgga gtcgggcttg cgcagcgcgt tctctgaatc catcaggcac
     2641 gccgggccgc aggattcggt gaggccatag acctgcagga tgctgatccc catctgcacg
     2701 tactgctcgg tcagggcgac gggtaccggc gcggcgccgg tcatgatcca gcgcagagcc
     2761 gagtagtcgt agcggtcctt cgcctctccg acctggagca tgaagttcag catggcggga
     2821 accatcaatc ccgaggtgat cttctcgcga tcgatgagct cccaggcgag cacgggatcg
     2881 aacgcgcgca tcacaaccga agtggccccg cgatagacgt tgacggccag aggtgtcagc
     2941 gcgccgacgt gaaacatcgg cagcgccccg agatagcggt cggtttcctc gtagtacgcg
     3001 ttcgccgcga tggtgaggat agcccagatg gccgtggtgt gagtgtggac gacccctttc
     3061 ggcagaccgg tggttccgga cgtgtacatg atgtagagca tgtcgtcgtc acaggccgtg
     3121 cagggcggct cctcggccgc cccgttgtcc cggaaagcct cgtagctctc agcgaagtgg
     3181 gcaacctcct gggaaccctc cacctgcagc cagtgccgga tgtcggtccg gtcgccgcgg
     3241 gcgtggagct cggcgacggt gtcgacgaaa tcctcgtcga agatcagccg cgccgtgccg
     3301 ctgtccttga ggatgaactc cagctcgtcg gcgacaagac gccaattgag gggaaccacc
     3361 accgccccta tcttggccag cgcaaaatag gcctccatga actccgcgct gttcatgagc
     3421 aggagaccca cgcgttcctc cttctgaacg cccgcctgca ggagcgaatt ggcgagctgg
     3481 ttggatcgtt cgttcagctg ccgaaacgtc agacgcaggc ccgaatggct gtcgacgtac
     3541 ccttcacgct ggggcgtcag aaacgcccgc ttcgtcagaa aaagaccgag attgttctcc
     3601 accgttgcct ccccttgcga gtaacccatc aaagcgcaag gccccctgca ggaactcaca
     3661 ggaccgcctc tcgagcccat tctcccgaac cagatcgtcc gtgtgaacac cctggtcgca
     3721 gtctccgccc aggtgaaaag attcgtaaca aaaaacccgg ggacgcgctt gatcactgcc
     3781 tgcagatccg tagaatgcgc gccttctttg cggacccgct cgtcggatct gcaaatgaaa
     3841 tcggcgtggc gcggtgtctc tctccccttc ctccctcacc gtgaagacag cgccaaaatg
     3901 cgctgtcgcc acgccacctt cctcctgcac tctctgcact ccctgcaccg gcgtcagacc
     3961 ggctacctgg acaaaatgac gttagcatgt acaattctgt acaggtattg tactggtacg
     4021 ctgcagggtg gtatcacgct ccgccatcga cgacgacctc tacaggatag gcacggtcgc
     4081 cacgctcacc ggcatctcgg tggagcgcct gcgtgcctgg gagcggcgtt acgggctggc
     4141 ccccacctac cgctcgggca agacgcgctt ctacagcaag cagcagcttc agacccttca
     4201 gcgcatcaaa cgtctgatcg accaggggca cccaatcagc agccttgcgc cgctttccga
     4261 cgagcaactt gacgaaagac tttccgctgc caccccccgg cttcaggccc tgcaatcggc
     4321 cacggtcggc ctgatcgggc cgaacctcct cgtgctggag caacagcagg gctcggacaa
     4381 ccgcctggaa gtccgcgccc gctgggcaaa catggacgct ttcatgagcg accagaccgg
     4441 caccgagcgg atcgacgtga tcgttgcgca gctgcccgtg ctgctcgtcg accacatcaa
     4501 caatctggcg cgtttccacc ctgaaagccg gatcgtcgcc atctaccagt tcgcaacgcc
     4561 ggcccagatc gccgccgtcc aggagctcgg catacccacc ctcacctggc cggcaggctg
     4621 gcaggagatc gagcatgcct gcgcgaccac ggccgggctg ccgctacgcg ccgcccgaac
     4681 ggccgtgcgc cgattcagcg acgcggagct catcgccatc tcggccagcg agcacgggga
     4741 tcccgcagcg tgccctcagc atctggtcga gctgatcagc gggctgaacg ccttcgccga
     4801 gtacacgttg aactgcgcgg atgaggccgc cgagccggag gtctaccaac gcgtgcacac
     4861 cgataccacc caggcacgcg cgcagctgga gctggctctg gagatgatgg tggatcaaga
     4921 ggcgggctga tcccatgagg ctcgacattg acctaaacaa aacgacctaa acaaaacctg
     4981 tacattttta ttgaaaatgg gttgacacag attttgtact gtacataata gccccactaa
     5041 cagttcaggc gtgcaacaca acttcatgca gtcctccctt tcaagtgtcc ttcccccaga
     5101 agagaagttg tttcagcggt gcccactgct gacacgcctt ttttacacgg actgagccgg
     5161 cagccggccg gatcagaccc acggtaacgc acaggcctct ttcagaggcc tgtgtcgttt
     5221 ccggccccag ccttttcgca acgccccagc cttttcgcaa cgttgaccgc ccgggcaacg
     5281 ccatcgtgct gcccggcgca cagagccggc acccgatctg tgcaaaaatc ggcggacccg
     5341 gccgtgatgg gcagaagtgg gcaaagatga ttcgccaagc attgccaaaa accgtgtgga
     5401 cggttacctg cgtattaacc ctgactgcct gtggcggggg cgggggcggc agcgacaccc
     5461 ccaccccggc ccccgctaac cggtcgccgg cggcatcctt cacggccacc ccgaccgagg
     5521 gaaccgtgcc tttaagcgtc gatttcgatg ccagcgggtc cagcgatgcg gacggacgca
     5581 tcgcaagcta cgcatggacc ttcggcgagc ctccaggagc atcgagcacc agcggcgtta
     5641 acacgtccca tacctacgag gaggccggca cctacaccgt agaacttacg gttaccgatg
     5701 acgacggcgc caccgggagc acgactcgca ccgtcacggt ggaggggcct gtcgcaagca
     5761 tcagcggtac cgtacgaatt ctggcctcca gcgccatcga cagcgacgtg aacgattttg
     5821 gcagcacgcc ggcgccgaac aacgactttg cca
//
LOCUS       DBNHOX010000217         4085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1753223, whole genome shotgun sequence.
ACCESSION   DBNHOX010000217 DBNHOX010000000
VERSION     DBNHOX010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1753223"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="AC16UZ_04785"
     CDS             <1..441
                     /locus_tag="AC16UZ_04785"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6246030.1"
                     /translation="PVQFMQAVIPGRVERGWGRVVNIGTGAAKFPTQIRILSGAPRAA
                     LVNYTVAVAKAVAKHNVMINNILPGMHHTATIHERYTAAAAANGTTYDEEVQKFVDEW
                     GIPANKFGDPDDVGAFVALFCSEFANYTTGQSLVIDGGITNATF"
     gene            502..1866
                     /locus_tag="AC16UZ_04790"
     CDS             502..1866
                     /locus_tag="AC16UZ_04790"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6246031.1"
                     /translation="MNQLVRSPDLPDWPKQQLRGHRIDGYRYTSREFFEKEFEHMWAK
                     VWLLLGRESEIPNPGDWQREDVGPESILMVRQQDGGIKAFYNVCQHRGNRLVSEEKGH
                     VSRFVCKYHSWAFMPDGELIHAQDADDFPEGNPCGKVNLLEIPCESFAGFIWVNMDPE
                     CESLREYLGPIWDDWERYEIEKWKRYVALTTVAPCNWKVVLDNFNESYHVPTVHKPMG
                     TAAERKRMHSGVDTNYKHTRFDLSDQGHNRMIMPGGYAGATMNSEGVIGEPLASIMRE
                     WQLDPNDFINRGQETRTALQEAKRRLGPERGYTHYARMADEMLTDAFHYTLFPNFAVS
                     LWSDGFHFLRARPHPTDPEQCVFDNWWYASQPAGETAPVRTTVSIVPRDTEVEHEVFR
                     FGEKSMGRTIDQDLAIFPEQQAGFRSRGYRGVYLSGQESRIRRYHELIDDYIEGRRPL
                     RKNI"
     gene            2008..>4085
                     /locus_tag="AC16UZ_04795"
     CDS             2008..>4085
                     /locus_tag="AC16UZ_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6246032.1"
                     /translation="MQVRGGKRWSSAALLVLLLSPIGAPAIAEERQAGREIEEIVVVS
                     TKRARGEAVQDIPIATTVVNADIISQNNMIDLIDVSRMVPNAQFKETATFPGIQRFWM
                     RAVGVTFSVPNFDPAVGVYQDGVFVAQNIAAILDTFDQESIEILRGPQGTLFGRNTSV
                     GAVLTRSRRPGDEFEFDAQVTLGSYDRNDYSLSVMGPIVKDVLSGKFAFMQRNVDEGW
                     VKNVAGGNTDPDVGRSERTHFKGALVWTPTENLDVTLLGEYFERDGDGALALPLGTED
                     NGKDGNPFIDARDWDETYDDEFPWETHSDHEITKVILEANLDVGHGIITAVSGFIDVD
                     AHSGSQFEGIPPFLITTRLQIDQDQFSQELRYASSFSDTFEFTAGAYYFTQDLKYGEQ
                     RAQGSRIGAAQPGITDPTNPFGLRAPTVDELDHDSWALFAEARLHLSDQFALIAGGRY
                     TDETKDVNMCLIYTGSCKGNETPPFIGSSGWTNGDGPENGWDIQDKESWKAFSPKIAI
                     EWRPQDDLMSYASWTRGFRSGGFSFRAGAAELTARDESIAAGEDPPFRPSYYDRERVD
                     QIEVGMKSDWFDNRLRLNLTGFYQWWDGIQRNLQEGPIGNIIQRTANVDDSHVYGFEV
                     ETNAILGLDMLTSGDSLRLDMALGWADSGYDSEYEVSGVDLSGDKFAAPHETYYFGLS
                     YNHPVGANME"
BASE COUNT          814 a         1255 c         1304 g          712 t
ORIGIN      
        1 ccggtgcagt tcatgcaggc cgtgattccc gggagggtgg aacggggttg gggcagggtg
       61 gtcaacatcg gcacgggagc ggcgaagttt cccacccaga tccgtattct gtcgggcgcc
      121 ccccgcgctg ccctggtcaa ctacaccgtg gcggtggcca aggccgtggc caagcacaac
      181 gtcatgatca acaacatcct gccggggatg caccacaccg cgacgatcca cgagcgttac
      241 acggccgccg cggccgcgaa cggcaccacc tacgatgagg aagtgcagaa gttcgtcgac
      301 gagtggggga tcccggcgaa caagttcggc gatccggatg acgtgggcgc tttcgttgcg
      361 ctcttctgca gcgagttcgc caactacacc acgggccaga gcctggtgat cgacgggggc
      421 atcaccaacg caacgttttg agcctgctgg accaaaaggg aaacgcgtta tactattccg
      481 cggattgagg agggtggttg attgaatcag ctggtacgca gccccgatct gcctgattgg
      541 ccgaagcagc agcttcgcgg ccacagaata gacggctacc gctacacgtc ccgggaattc
      601 ttcgaaaaag aattcgagca catgtgggcc aaggtctggc tgctgctcgg gcgagaatcg
      661 gagattccga atccgggcga ctggcagcgc gaggacgttg gtcccgagtc catcctcatg
      721 gtccgccagc aagacggcgg catcaaggcc ttctacaacg tctgccagca ccgcggcaat
      781 cggctcgtct ccgaggagaa gggccatgtg agccgcttcg tctgcaagta ccacagctgg
      841 gcgttcatgc ccgacggcga gctgatccat gcccaggacg cggacgactt ccccgaaggc
      901 aacccctgcg gcaaagtcaa cctgctggag attccctgcg agtcgtttgc cggcttcatc
      961 tgggtcaaca tggatccgga gtgcgaaagc ctgcgtgagt atctcgggcc catctgggac
     1021 gactgggagc gctacgagat cgagaagtgg aagcgctacg tggcgctcac cacggtagcg
     1081 ccgtgtaact ggaaggtggt gctcgacaac ttcaacgagt cctaccacgt gcccacggtg
     1141 cacaagccca tgggcactgc agcggagcgc aagcgcatgc actcgggtgt ggacaccaac
     1201 tacaagcaca cgcgcttcga cctgtccgac cagggtcaca accgcatgat catgcccggc
     1261 ggctacgcgg gcgcgaccat gaacagcgag ggtgtgatcg gtgagccgct ggcctcgatc
     1321 atgcgcgagt ggcagctgga tccgaacgac ttcatcaacc ggggccagga gacccgcacg
     1381 gcgctgcagg aggcgaagcg ccgactgggg cctgagcggg gctacaccca ctacgccagg
     1441 atggccgacg agatgctcac cgacgccttc cactacacgc tgttccccaa cttcgcggtg
     1501 tctttgtggt ccgacggctt tcacttcctg cgggcccgtc cgcacccgac agacccggag
     1561 cagtgcgtct tcgacaactg gtggtacgcg agccagcctg cgggggaaac cgcgccggtg
     1621 cggaccaccg tcagcatcgt gccccgcgat acggaagtgg agcacgaggt gttcagattt
     1681 ggcgagaagt ccatgggtcg caccatcgac caggatctag ccatcttccc cgagcagcag
     1741 gcgggcttcc gctcgcgcgg ctaccggggc gtttatctgt ccggccagga aagccggatc
     1801 cgtcgctatc acgagctgat cgacgactac atagagggga gacggcccct tcgaaagaac
     1861 atctgaggcg gtcttcggcg ggcccaggcg cggccccgtc aacgtccgcc ccaatgaaag
     1921 cctgcccggc ggtctcggga agcgacctct gggcggcttg cggaaagatc ggttggagcc
     1981 ggtcaacaaa cacactggag agttgatatg caagtaagag gagggaagcg ctggtcgagc
     2041 gctgcattgc tagtgctgct gttgtcgccg attggcgcgc cggcaattgc ggaggagcga
     2101 caggccggtc gtgagatcga agagatcgtg gtcgtgtcca ccaaacgcgc ccgcggtgaa
     2161 gcggtacagg atattcccat cgccaccacc gtggtgaacg cggacatcat cagtcagaac
     2221 aacatgatcg atctgatcga cgtctcgcgc atggtgccga acgcgcagtt caaggagacg
     2281 gccacctttc ccggcattca gcggttttgg atgcgggccg taggggtgac gttcagtgtt
     2341 cccaatttcg accccgcggt gggcgtttac caggatggcg tttttgtcgc gcagaacatt
     2401 gctgccatcc tggatacctt cgatcaggag tccatcgaga tcctcagggg cccccaggga
     2461 accctgtttg ggagaaatac ctcggtgggc gcggtgctca cccggtcacg ccgtccgggc
     2521 gacgagttcg agttcgacgc tcaggtcacc ctgggttcct acgatcgcaa cgactactcg
     2581 ctctccgtca tgggaccgat cgtcaaggac gtgctgagcg ggaagttcgc gttcatgcag
     2641 cgcaacgtcg acgagggttg ggtgaagaac gtggccggcg gtaacaccga ccccgacgtg
     2701 ggccgcagcg agaggaccca cttcaaaggc gccctggtct ggacgccgac ggaaaatctc
     2761 gacgtcaccc tgcttggcga gtatttcgag cgcgacggtg acggggcgct ggccctgccg
     2821 ctcggtaccg aggacaacgg caaggacggc aaccccttca tcgacgcgcg agattgggac
     2881 gagacctacg acgacgaatt tccgtgggaa acccattcgg accacgagat caccaaggtc
     2941 attctcgagg ccaacctgga cgtcggccac ggcatcatca ctgcggtttc gggcttcatc
     3001 gacgtggacg cacactccgg ctcgcagttc gaaggcatcc cgccgtttct tatcaccacg
     3061 cggctgcaga ttgatcagga tcagttcagc caggagctgc gctacgcttc gtctttctcc
     3121 gataccttcg agttcaccgc cggcgcctac tacttcactc aggacctgaa gtacggcgag
     3181 cagcgggccc agggctcccg tatcggcgcc gctcagccag ggatcacgga tcccactaac
     3241 cccttcggcc tcagggcgcc aacggtggac gagctggacc acgactcctg ggctctgttt
     3301 gccgaggctc ggctgcacct gagcgatcag ttcgctctga ttgccggcgg ccgttacacg
     3361 gacgagacca aggacgtgaa catgtgcctg atctacacgg gctcatgcaa aggcaacgag
     3421 acgccgcctt tcatcggctc ttcgggctgg accaacgggg acgggccgga gaacggctgg
     3481 gacattcagg acaaagaatc ctggaaggct ttctcgccga agatcgccat cgagtggcgg
     3541 cctcaggatg acctgatgag ctacgctagc tggacccgcg gattccgcag cggtggcttc
     3601 tctttccggg cgggcgccgc tgagctcacg gcccgggacg agagcatcgc ggcaggggag
     3661 gatcctccct tccggccttc ctactacgat cgcgagcggg tggatcagat cgaggtcggc
     3721 atgaagtccg actggttcga taatcggctg cggctgaacc tgaccggctt ctaccagtgg
     3781 tgggacggca tccagcgtaa cctgcaggaa gggcccatcg gcaacatcat tcagcggacg
     3841 gccaacgttg acgactctca cgtgtacggg ttcgaggtcg aaaccaacgc cattctgggg
     3901 ctggacatgc tgacgagtgg tgacagcctg cgcctcgaca tggcacttgg ctgggccgac
     3961 tcgggctacg actcggagta cgaggtcagc ggcgtcgacc tcagcggcga caagtttgcc
     4021 gctccgcacg agacctacta cttcgggttg agctacaacc acccggtggg cgccaacatg
     4081 gagat
//
LOCUS       DBNHOX010000218         3161 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_597078, whole genome shotgun sequence.
ACCESSION   DBNHOX010000218 DBNHOX010000000
VERSION     DBNHOX010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_597078"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="AC16UZ_04800"
     CDS             complement(<1..206)
                     /locus_tag="AC16UZ_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014795.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR6246033.1"
                     /translation="MATLDGTTLIARSLKQQGVEYMFGVVGFPVFGIAAAAQAEGITY
                     IGMRNEQSASYAAQAAGYLTGRPQA"
     gene            complement(315..1910)
                     /locus_tag="AC16UZ_04805"
     CDS             complement(315..1910)
                     /locus_tag="AC16UZ_04805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR6246034.1"
                     /translation="MTTYDVAIIGGGHNGLVCAAYLAGAGLDVIVLEKRGVVGGAAVT
                     EEFHPGFRNSVASYTVSLLNPKVIQDLRLAEHGLRIVNRPYSNFMPLPDGDYLRTGAT
                     IEETQQQFARFSRTDAERLPGYYAALEAGADLLRGMLLETPPNAGGGLRDVMRAVRLG
                     GRLRRLSTAARRDVLDLFTKSAGELLEYWFESDPVKALFGFDAVVGNYASPYAPGSAY
                     VLLHHVFGEANGQAGQWGHAIGGMGAITQAMLKEALARGVTVRTDADVRLVRVRNGRA
                     NGIVLEDGEEIGARAVASNLNPKLLFTRLIEAEALDDDFRARIARYRCGSGSFRINVA
                     LSQLPRFECAPPGQPELGTRSGIIMAPSLKYMERAYFDAREHGWSRKPIVEMLIPSTV
                     DDSLAPPGQHVASLFCQQFAPTLDGQSWAEARDAAAQTVFDTVEGYAPGFRQSILGAM
                     VLSPEDLEAEFGLTGGDIFHGALSLDQLFSARPVLGHGDYRAPVRGLYMCGSGTHPGG
                     GVTGAPGHNAAREIIRDFRARRL"
     gene            complement(1947..2747)
                     /locus_tag="AC16UZ_04810"
     CDS             complement(1947..2747)
                     /locus_tag="AC16UZ_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246035.1"
                     /translation="MEATPLLMGIRARHALWFDCAMGFAALAVVCLLMLAMDERALNG
                     VNVWAKPLKFSISLAVYFATLAWFAPLMPDGYFAGTGGRWLSAIPVACAVFEIVYIIV
                     QAALGEPSHFNVSTTFHSTMYTLMGGGAFILVAICLWMGLIILSIHRMSNPYALSVGL
                     ALVLTFVLGGGFGAYLGGQTGHWVGGTLSDADGLWLVNWSRDGGDLRVPHFFGMHAMQ
                     ILPALATLVPHRMTRDGANVAVLAMTLLYAASAGWTFQQAVSGIPFAG"
     gene            complement(2789..>3161)
                     /locus_tag="AC16UZ_04815"
     CDS             complement(2789..>3161)
                     /locus_tag="AC16UZ_04815"
                     /inference="COORDINATES: protein motif:HMM:NF025473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABA4-like family protein"
                     /protein_id="HIR6246036.1"
                     /translation="RRFVVQILARVLIPAIIAVIYIGLMVGHVGSFPEGGGFGSLAGI
                     ATLFSVDALLLAGWIHYLAFDLFVGSWEVTDSRKEGIPHLLLVPCLVLTFMAGPAGLA
                     LYFAIKYGRRSLASEAAQPSQ"
BASE COUNT          562 a         1041 c         1071 g          487 t
ORIGIN      
        1 gcctggggac ggccggtgag gtagccggcc gcctgagcgg cgtagctcgc ggactgctcg
       61 ttacgcatgc ctatgtaggt gatgccctcc gcctgagcgg cggcggcgat gccgaacacc
      121 ggaaagccca cgacgccgaa catgtactcg acgccctgct gcttgaggct ccttgcgatg
      181 agcgtagtgc cgtctaaggt tgccatgatt ccctccctcg ctttgttatc tgcttctctg
      241 cggtggctct ggcgtgcccc gaggggccga gtatagcaag gccgccggcc cctctcgctt
      301 cgaggtgtgt ccgttcagag ccggcgcgcc cggaagtcgc ggatgatctc ccgggcggcg
      361 ttgtggcccg gtgccccggt caccccgccc ccgggatggg tgccggagcc gcacatgtac
      421 aggccccgca cgggggcgcg atagtcgccg tggccgagca ccggccgggc cgagaagagc
      481 tggtccaggg acagggcgcc gtgaaagata tccccgcccg tgagcccgaa ctcggcctcg
      541 aggtcctccg gggagagcac catggcgccg agaatgctct gcctgaaccc gggcgcgtag
      601 ccctccaccg tgtcgaagac ggtttgcgca gccgcatcgc gggcctcggc ccagctttgc
      661 ccgtccagcg tgggggcgaa ctgttggcag aagaggctgg caacgtgctg ccccggcgga
      721 gcaagcgagt cgtccaccgt gctgggaatg agcatctcga cgatgggctt ccgggaccag
      781 ccatgctcgc gggcgtcaaa ataggcgcgt tccatgtact tgaggctcgg cgccatgatg
      841 atgcccgacc gggtgccgag ttccggctgg cccggtggag cgcactcgaa gcgcggaagc
      901 tggctcaagg ccacgttgat tcggaaggaa cccgatccgc atcgatagcg ggcaatacgc
      961 gccctgaagt cgtcgtccag cgcctcggct tcgatgagcc gggtgaacag cagctttggg
     1021 ttgaggttcg acgctaccgc ccgggcgccg atctcttccc cgtcttcgag cacgataccg
     1081 tttgcacgac cgttgcggac gcgaacgagc cgcacgtcgg cgtccgttct caccgttact
     1141 ccccgagcga gcgcctcttt gagcatcgcc tgggtgatgg cgcccatgcc cccgatcgcg
     1201 tgtccccact gacccgcctg gccgttggct tcgccgaaaa cgtggtgcag cagaacgtag
     1261 gccgatcccg gggcgtaggg gctcgcgtag ttgccgacga cggcgtcgaa gccgaacagc
     1321 gccttgaccg gatcggactc gaaccagtac tcgagcagct cgccggcgct tttcgtgaac
     1381 agatcgagca catcgcgtcg cgctgcggtg gagaggcggc gcaggcggcc gccgagacgg
     1441 acagcgcgca tcacgtcgcg aagcccgccg ccggcgttgg gcggggtttc cagaagcatg
     1501 cctcgcagca gatcggcgcc ggcctcgagc gcggcgtagt acccgggaag gcgttccgcg
     1561 tctgtccggg agaaccgggc gaactgctgc tgagtttcct cgatggtggc ccccgtgcgc
     1621 aggtagtctc cgtcggggag cggcatgaag ttcgagtatg gccggttgac gatgcgcagg
     1681 ccgtgctcgg cgagcctgag atcctggatg accttcgggt tgagcaggct gaccgtgtag
     1741 ctggccacgg aattgcggaa gcctggatgg aactcctccg tgacggcagc cccgcccacc
     1801 acgccccgtt tctccaggac gatcacgtcc aggccggcgc cggccagata agccgcgcag
     1861 accagcccgt tgtgaccgcc gccgatgatc gctacgtcgt acgttgtcat gccctccggc
     1921 gcccccgtgg acgggcactc taatcgctag ccggcgaacg gtataccgct cacggcctgc
     1981 tggaacgtcc agccggcaga ggcggcgtaa agcagggtca tggcgagcac cgcgacgttc
     2041 gccccgtccc gggtcatgcg gtgagggacc agggtggcca gagccggcag gatctgcatg
     2101 gcgtgcatcc cgaaaaagtg cggcaccctt agatcgccgc cgtcccgcga ccagttcaca
     2161 agccaaagac cgtcggcgtc ggagagcgtg ccgcccaccc agtgcccggt ctggccgccc
     2221 aggtaggcac cgaagccacc gccgagaacg aacgtcagca cgagggcgag gcccaccgag
     2281 agcgcgtagg ggttcgacat ccggtggatg ctcaggatga tcaggcccat ccacaggcaa
     2341 atggcaacga ggatgaaggc gccgcctccc atcagcgtgt acatcgtgct gtggaacgtt
     2401 gtggagacgt tgaagtgcga tggctcaccc agggccgcct gcacgatgat gtagacgatc
     2461 tcgaagacgg cgcaggccac agggatggcg ctgagccagc gaccgccggt tcctgcgaaa
     2521 tagccgtcgg gcatgagcgg cgcgaaccag gccagcgtgg cgaagtaaac ggcgagggag
     2581 atagagaact tcaggggctt ggcccagacg tttaccccgt tcagggcgcg ctcgtccatg
     2641 gcaagcatca gcaggcagac gacggccagg gcggcaaacc ccatggcgca atcgaaccag
     2701 agggcgtggc gcgcgcgaat gcccatgagc agcggggttg cctccaccgg cgattcccct
     2761 gccaataccg gctgccgccc cctttcgctc attggctcgg ctgtgctgcc tcgctcgcca
     2821 gcgatcggcg tccgtacttg atggcaaagt aaagagccaa cccggcggga ccggccatga
     2881 aggtcaagac caggcagggc accaggagca ggtggggaat gccttctttc cgggaatccg
     2941 tcacctccca ggaacccacg aacagatcga atgccaggta gtgaatccac ccggccagga
     3001 gcagtgcgtc cacgctgaag agcgtcgcga tgcccgccag cgagccgaac ccgccaccct
     3061 ccgggaagct gcccacatgg ccgaccatca ggccgatgta gatcacggcg atgatggccg
     3121 ggatcagcac ccgggccaga atctgaacca cgaaccgccg g
//
LOCUS       DBNHOX010000219         2333 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_982546, whole genome shotgun sequence.
ACCESSION   DBNHOX010000219 DBNHOX010000000
VERSION     DBNHOX010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_982546"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="AC16UZ_04820"
     CDS             <1..358
                     /locus_tag="AC16UZ_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HIR6246037.1"
                     /translation="VNFGIDVINQIKLETPGLWDEELQTLARQMILEGTQLELEYAKD
                     TMPRGILGMNANMMAEYLQFIANRRLAQIGLAEQFAGAKNPFPWMSEIMDLKKEKNFF
                     ETRVTEYQTGGALSWD"
     gene            complement(383..730)
                     /locus_tag="AC16UZ_04825"
     CDS             complement(383..730)
                     /locus_tag="AC16UZ_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246038.1"
                     /translation="MAGMRGLVFGMLMFLSASSAAREMQVEGDVVRVIPLTASSVVVE
                     RSGDCAPVKPIGQADLVALLAWDLRADCHTQRRKVEVQDGYRVYYRWDERVYSVVMTE
                     RPGPTIPLRVNVR"
     gene            complement(733..1428)
                     /locus_tag="AC16UZ_04830"
     CDS             complement(733..1428)
                     /locus_tag="AC16UZ_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR6246039.1"
                     /translation="MSRAAPLLAALLLCGTGHAVTQYPYLSCFEAASRLHDVPVDLLL
                     AVAATESNWDPDARSAANAHGIMQIQWPGTARHLGVGRLSELYNPCLNIERGAKYLSE
                     LLARSGGAEDRALAAYNYGPSRIASSSTLPAGALRYVSTVQGHRERIVRKTGARQASA
                     TRAAEHAVRFDTAHRARRYARSLDERVEGADFVTRAEPEGGFAVVMRIAAGGLSLTDL
                     NVLERLGWPVRGD"
     gene            complement(1425..2039)
                     /locus_tag="AC16UZ_04835"
     CDS             complement(1425..2039)
                     /locus_tag="AC16UZ_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246040.1"
                     /translation="MKFLLGLILGGAMVVVAAQRLAVDGPDSGVSSLGAWLTGMRAAA
                     GQFEPGWAAREDQPTALPAQTDPAAGDAEVDAESLGAGPAEVAEGPLAADMQEPMVRL
                     AGPEPLPPAEGPEPLPPAVRLLPAADPPAGSASETIQQVWAPFRSQTSAEGFARRLSS
                     ETEHPFGIDRRGPGRYQVFFAYADEEERRRLLEQVRPLIGTDAP"
     gene            complement(2049..>2333)
                     /locus_tag="AC16UZ_04840"
     CDS             complement(2049..>2333)
                     /locus_tag="AC16UZ_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246041.1"
                     /translation="AEPGPRRYRIRVPSGAATASRPDAGVYVLAVEKRSVAHRIARQI
                     NAGPGACPGGVPESTGQWLQALGRVLAEYGGDVDWRAAHFRGGPGGITRI"
BASE COUNT          414 a          815 c          757 g          347 t
ORIGIN      
        1 cgtgaacttc ggcatcgatg tgatcaatca gattaagctc gagaccccgg ggttgtggga
       61 cgaggaattg cagactctcg cccggcagat gatcctcgag ggtacccagc tggaactcga
      121 gtacgccaag gacaccatgc caagaggaat actcggcatg aacgcgaaca tgatggccga
      181 gtacctccag ttcatcgcaa accggcgtct ggcgcagatc gggcttgccg agcagtttgc
      241 gggagcgaag aacccgttcc cgtggatgtc cgagatcatg gatctcaaga aggagaaaaa
      301 cttcttcgag acccgggtca ccgagtacca gactggcggg gcgctcagct gggattgagg
      361 gcagctgagg gcgccgccgc cctcagcgaa cgttgacgcg aagcgggatc gtgggtcccg
      421 gtctttcggt catcacgacg ctgtaaaccc gctcgtccca tcggtagtac acgcggtaac
      481 cgtcctggac ctccaccttg cgtcgctgcg tgtggcaatc ggcccgcaga tcccaggcca
      541 gcagagcgac cagatcggct tgcccgatcg gcttgacggg cgcgcagtcg ccgctgcgtt
      601 cgaccaccac cgacgaggcg gtgagcggaa tcactcgaac gacgtcgccc tctacctgca
      661 tttcccgggc agccgacgag gcgctcagga acatcagcat cccaaagacc aatccacgca
      721 tccccgccat cgtcaatctc cccgtaccgg ccaaccgagc cgttccagca cgttcagatc
      781 ggtaaggctc agcccaccgg ccgcgatgcg catcaccacg gcaaacccgc cttccggctc
      841 ggcgcgcgtc acgaaatcgg caccttcaac gcgctcgtcc agcgaacggg catagcggcg
      901 ggcacgatgc gccgtatcga agcgcaccgc gtgctcagcg gctcgcgtcg cagacgcctg
      961 gcgcgctccg gtcttccgga cgatgcgctc gcgatggccc tgcaccgtcg agacgtacct
     1021 cagggcgccg gccggcagcg tgctgctgga ggctatcctg ctcgggccgt agttgtaggc
     1081 cgcaagcgcc cggtcctcgg cgccgccgct tcgcgcgagc agctcactca gatatttcgc
     1141 gccgcgctcg atgttcagac aggggttgta cagctcggag agtcggccga caccgagatg
     1201 ccgggcggta cccggccact ggatctgcat gatcccgtgg gcgttcgcag cggatctggc
     1261 atccggatcc cagttgctct ccgtagctgc cacggccagc agcaggtcca caggcacgtc
     1321 gtgcagccgg gaagccgcct cgaaacagga aagatacgga tattgcgtga ccgcatgacc
     1381 ggtaccgcag agcagcagag cggccagcag gggcgcggcg cggctcatgg cgcatccgtc
     1441 ccgatgagcg gccgcacctg ctcgagaagc cggcgccgct cctcctcgtc agcgtacgcg
     1501 aaaaacacct gataacgacc gggtccccga cgatctatcc cgaaggggtg ctccgtctcg
     1561 ctgctcaagc gcctggcaaa accttccgcg gacgtctggc tgcgaaacgg cgcccagacc
     1621 tgctggatgg tttcggaagc ggaccctgca ggcgggtccg cagcgggcag caagcgcacg
     1681 gcaggcggga gcggttccgg gccttcggcc ggcgggagcg gttccgggcc agcaagccgc
     1741 accatgggct cctgcatgtc ggcggccagc ggcccctcgg caacctcagc gggacccgcg
     1801 cccagactct cagcgtccac ttcggcgtcg cccgcggcgg gatcggtctg cgcgggcagg
     1861 gcggtcggct ggtcctcgcg ggcagcccag cccggctcga actggccggc cgcggcgcgc
     1921 ataccggtca gccaggcacc caggctcgag actcccgagt cgggcccgtc gactgccaag
     1981 cgttgcgcag ccaccacgac catcgcgccg cccaagatca gtccaagcaa gaatttcatg
     2041 acgcctcctc agatcctcgt tatgccgccg gggccgccgc gaaagtgggc ggcgcgccaa
     2101 tccacgtcgc cgccatactc ggcgagaacg cggcccaacg cctggagcca ctggccggtg
     2161 ctttccggga cgccgccagg acaggctccg ggtccggcgt tgatctgccg cgcgattcgg
     2221 tgggcgacgc tgcgcttctc caccgcaaga acgtaaacgc ccgcatccgg gcggctggcc
     2281 gtcgccgccc cggagggaac ccggattcgg tatcgccggg gcccgggctc ggc
//
LOCUS       DBNHOX010000220         9172 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1238752, whole genome shotgun sequence.
ACCESSION   DBNHOX010000220 DBNHOX010000000
VERSION     DBNHOX010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9172
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1238752"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="AC16UZ_04845"
     CDS             complement(<1..736)
                     /locus_tag="AC16UZ_04845"
                     /inference="COORDINATES: protein motif:HMM:NF015413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR6246042.1"
                     /translation="MDFQEQLSEVIRQYGSGSLDETRFLLESLRAPEIANLMESTPPR
                     VRRVIWGLLDEEDENQVIQHLREDVRADFLESMNTERLVAAGVDLEVDDFADILQQLP
                     ATLSEQVLASLDQSDRARVETVLSYPEDSAGGIMNTDAITVRPRHSVELVLRYLRLRK
                     DLPEATDALIVVNQRDEYVGMVSLASLITSDPTVTVREVMNTEARSIPADTPDTEVAR
                     LFSEQDLISAPVVDQQGRLIGRITIDD"
     gene            complement(788..1057)
                     /locus_tag="AC16UZ_04850"
     CDS             complement(788..1057)
                     /locus_tag="AC16UZ_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR6246043.1"
                     /translation="MREGRITIVNRLGLHARAAAKFVNLAKTFSSQVELTRDAERVDG
                     KSIMSVMLLAAPIGTELILHVVGPDEDEAFEALEQLVDDRFGEAE"
     gene            complement(1065..1919)
                     /gene="rapZ"
                     /locus_tag="AC16UZ_04855"
     CDS             complement(1065..1919)
                     /gene="rapZ"
                     /locus_tag="AC16UZ_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR6246044.1"
                     /translation="MKLIVISGRSGSGKSTALRALEDSGYNCIDNFPVDLLHHLIANA
                     TRAVEPQPDLAVCIDARNPDLEAFPQIIESLRSPSVDCEVIFLDAQSATLVKRFSETR
                     RRHPLTGTGKDLLQAIESEDAILQPIAGLADLSIDTTTMRGSALTELVRSRIAHKRSN
                     SLSLLFRSFGFKFGVPVDADFVFDIRCLPNPYWDESLRPLTGKDGAVASFLEAEAAVG
                     QMFSDIQSFLERWIAAFEKDNRAYMNIALGCTGGQHRSVYMAEKLAAHFSAQRPNVLV
                     RHRELAGS"
     gene            complement(1916..2383)
                     /locus_tag="AC16UZ_04860"
     CDS             complement(1916..2383)
                     /locus_tag="AC16UZ_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR6246045.1"
                     /translation="MPDITTILRPDCTRASVEAPSRKRALEAAADLVAKHHPDLSARK
                     LFDELMARERLGSTGLGDGVAIPHCRIPCEQITGALLHLREAVDYESMDDEPVDLLFV
                     LAVPPEETTAHLDLLAALARIFGDPENRRRLRSSATDEALYQELVGLFSSQAA"
     gene            complement(2393..2698)
                     /gene="hpf"
                     /locus_tag="AC16UZ_04865"
     CDS             complement(2393..2698)
                     /gene="hpf"
                     /locus_tag="AC16UZ_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR6246046.1"
                     /translation="MQLSISGHHIDVTEALRNYVTEKMQKLELHFDHITNVHVVLSVE
                     KLRHRAEATIHASGTEVFADCDCDDLYAAIDGLTDKLDRQVLKHKEKLLDRQQRRGR"
     gene            complement(2755..4230)
                     /locus_tag="AC16UZ_04870"
     CDS             complement(2755..4230)
                     /locus_tag="AC16UZ_04870"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR6246047.1"
                     /translation="MKQTLSLKLGQQLTMTPQLQQAIRLLQLSCLDLRQEIQDALEGN
                     PMLELDEEDGGDEPTGSPAEADRAESDGDDDLDGVDATLEGDVIDDLPVDTSWDEIYQ
                     PAPAPAPAAGDSESWDFETRNAPEETLQDHLLWQLNLTPMSDSDRVIAMAIIDTISPD
                     GMLTTPVEELVTSLEGVVDEEAEIAHAEVMAVLKQIQHFDPIGVGARDLGECLRIQLE
                     QLPAGTPWLAESLTLVNEHLPLLASRDFAALKRRTKLGEEELGEVVRLIQTLNPRPGT
                     TVAQEAAEYVVPDVMVSRKEGRWLVELNPDATPGLRINAGYAGLVKRADNSADNTFLR
                     DHLQEARWFLKSLQSRNETLLKVATRIVEHQRGFLEYGEEAMKPLVLNEIAEAVEMHE
                     STISRVTSRKYMHTPRGIFELKYFFSSHVSTSSGGEVSSTAIRALIKKLTADENPRKP
                     LSDSRIATILAEQNINVARRTVAKYRESMAIPPSNERKRLV"
     gene            complement(4391..5116)
                     /gene="lptB"
                     /locus_tag="AC16UZ_04875"
     CDS             complement(4391..5116)
                     /gene="lptB"
                     /locus_tag="AC16UZ_04875"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003313594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR6246048.1"
                     /translation="MPTLEVRSLRKQYRNKVAVEDVSLHVSTGEIIGLLGPNGAGKTT
                     TFYMIVGLLRADGGDIFLDEQRITRTPMHVRARAGIGYLPQEASVFRKLSTEDNVRAI
                     LELRRDLNRTQRQAMLDQLIEEFHLGGVRESLGERLSGGERRRLEIARALAMEPSFML
                     LDEPFAGVDPISVGDIKKEIRDLAARGIGVLITDHNVRDTLDICDRAYIVHEGHIIAE
                     GDSGAILADEKVKNVYLGESFSL"
     gene            complement(5125..5607)
                     /gene="lptA"
                     /locus_tag="AC16UZ_04880"
     CDS             complement(5125..5607)
                     /gene="lptA"
                     /locus_tag="AC16UZ_04880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HIR6246049.1"
                     /translation="MTLGAVLLAPGALALPEDADQPIHIRANSAEMDQEQGLVIYRGD
                     VQVDQGTLRVMADEMTVEYEDQEVIRITAKGEPARYQQQIEVDEEVVRARSSTIIYHT
                     RDERIDLVGNAFLTQEGNEITGDLIEYDIVAGKVDAKAESETPVRMILQPARKKPDGG
                     "
     gene            complement(5621..6250)
                     /gene="lptC"
                     /locus_tag="AC16UZ_04885"
     CDS             complement(5621..6250)
                     /gene="lptC"
                     /locus_tag="AC16UZ_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR6246050.1"
                     /translation="MRKRSKWERWSTWTAILGVAVAGVVLLSEPLEPETEAETPPAGI
                     AEEPDLYVEDAVITQYLPSGTVHYRLVSKLLRQFEADELVRLTKPNLVVNSETRSPWQ
                     VQSEHGYVRRMGGPERGGEGEEVVFLREDVQLEQREPDGRHIRLTTETLYVYPEREYA
                     ETDRDVMIDTHAGRTTATGLKGDLQRGLLQLSSSDRRRVHTILLPDQFK"
     gene            complement(6262..6777)
                     /locus_tag="AC16UZ_04890"
     CDS             complement(6262..6777)
                     /locus_tag="AC16UZ_04890"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HIR6246051.1"
                     /translation="MAFPAPVLERAADIELVAFDVDGVLTDGKLYYSDDGRELKAFHV
                     QDGAALKLLLSHGIVVAIITGRSSSIVTRRADELGIAHVYQGVADKAAVLAALAETVG
                     TTLERAAYVGDDLPDLGVFSQAGMAISVPNAHPAAAAAADYVTLAPGGAGAAREIAQL
                     FLEARGEWPYG"
     gene            complement(6777..7748)
                     /locus_tag="AC16UZ_04895"
     CDS             complement(6777..7748)
                     /locus_tag="AC16UZ_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HIR6246052.1"
                     /translation="MLLSIAALIVGFMALLWSADRFLAGAASTAGHLGVSKMLIGLTV
                     VSLGTSAPEILVALTAAVEGNPLLAVGNAIGSNIANIGLVLGITAIIVPLPFSQTVLR
                     AELPWLLGATFLALALLFDLEMSALDSVLLLAGLGVILWRLVRSQKTAGSDLAESITE
                     ELEDLPQMSRGQAAVWLLIGLLILLASAEVLVWAASDIADRLGVNDIIIGLTIVAIGT
                     SLPELAATVGSALKGHTDIAIGIVVGSNILNILAVLAVPSLVDPILIENVWLWRDGGM
                     MLALTLLLAVFAYAVGSQPVITRFEGTVMLCAWVGYNLLLVSQAQLG"
     gene            7877..8692
                     /locus_tag="AC16UZ_04900"
     CDS             7877..8692
                     /locus_tag="AC16UZ_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6246053.1"
                     /translation="MNGADDNLIEIRNLSFVRGDRVIFDDLSLSVPRGKITAIMGPSG
                     TGKTTLLRIIGGQVRPDSGVVLIDGQDVCGMSRRELFELRKDMGMLFQSSALFTDLNV
                     FENVAFPLRVHTDLPEDTVRDLVLMKLNAVGLRGARDLMPSQLSGGMSRRVALARAVA
                     LDPNLLMYDEPFTGQDPIAMGVLLSLIESLNDSLKTTSLVVSHDIHETISIADQIYLI
                     ADGTIMGSGTPDTLAVDDSPRVHQFMHGSPDGPVPFHYPADEFLYDVLGDVNA"
     gene            8734..>9172
                     /locus_tag="AC16UZ_04905"
     CDS             8734..>9172
                     /locus_tag="AC16UZ_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007352398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6246054.1"
                     /translation="MASLGRATIMWFTALAGLPGPGDLRLLVRQIYHVGVLSLIIIVL
                     SAFSIGAVIALQFHTQLARVGAEDSVGLGLALVLLRELGPVVTGLLFAGRAGSAITAE
                     IGLMKTTEQLSSMEMMGVDPLRRIVAPRLWAGVISMPLLTAIFN"
BASE COUNT         1473 a         2929 c         3005 g         1765 t
ORIGIN      
        1 cgtcgtcgat ggtgatccgg ccgatcaggc gcccttgctg atccacgacg ggcgcggaga
       61 tcagatcctg ctcggagaac aggcgcgcaa cctcggtgtc cggcgtgtcg gcggggatcg
      121 accgcgcttc ggtattcatc acctcgcgaa cggttacggt ggggtccgag gtgatcaggc
      181 tggccagcga caccataccg acgtactcgt cccgctggtt cacgacgatg agcgcgtcgg
      241 tggcctccgg caggtccttt cgcaggcgca ggtagcgcag caccagctcc acggagtgcc
      301 gtggccgcac ggtgatcgcg tcggtgttca tgatgccgcc ggcactgtcc tccggatagg
      361 agagcacggt ctccacccgg gcccggtcgc tctgatcgag ggatgccagg acctgctcgg
      421 aaagggttgc cggcagctgc tggaggatgt ccgcgaagtc gtcgacctcc aggtcgacgc
      481 cggcggccac cagccgctcg gtgttcatgg actccagaaa atcggcccga acgtcctcgc
      541 ggagatgctg aatgacctgg ttctcgtcct cttcgtccag gagcccccag atgacccggc
      601 ggactctggg cggggtggac tccatgaggt tggcgatttc cggcgcgcgc agggattcga
      661 gcagaaatct ggtctcgtcc agacttccgc tgccgtattg gcggatgact tcgcttagct
      721 gttcctgaaa gtccacgcgg gtaccgaaac ttgagggcgc gcgcagatta ccagatgcgc
      781 cggccgttca ctcagcttct ccgaagcggt cgtcgacgag ttgctccagg gcttcgaagg
      841 cctcgtcctc gtccggcccg acgacgtgca ggatcagctc ggtgccgatg ggcgcggcga
      901 gcagcatgac gctcatgatg cttttgccgt ccacgcgctc ggcgtccctc gtcaactcca
      961 cctggctcga gaaggtcttt gccagattga cgaacttggc cgcggcccgg gcgtgcagcc
     1021 cgagacggtt gactatggtg atccttccct cccgcatggc ggggttacga acctgccagc
     1081 tcgcggtgcc tgaccagcac gttgggccgc tgggccgaga agtgggcggc aagcttttcc
     1141 gccatgtaca cgctgcggtg ctggccgccg gtgcagccca gggcgatgtt catgtacgcc
     1201 cggttgtctt tctcgaaggc ggcaatccag cgttccagga acgactggat gtccgagaac
     1261 atctggccaa cggccgcttc cgcttccaga aacgacgcca ccgccccgtc cttgccggtc
     1321 agcggacgca gggactcgtc ccagtaggga ttgggcaggc agcggatgtc gaagacgaag
     1381 tcggcgtcca caggcacacc gaacttgaac ccgaatgatc ggaacagaag agagagcgag
     1441 ttgctgcgct tgtgggcgat gcggctgcgc acgagctcgg tcagggcact gcctctcatg
     1501 gtggtggtgt cgatgctgag atccgccagg ccggcaatcg gttgcaggat cgcatcctcc
     1561 gattcgatgg cctggaggag gtccttaccc gtgccggtca gcgggtggcg ccggcgggtc
     1621 tcgctgaagc gtttgaccag cgtcgcgctc tgggcgtcaa ggaagatcac ctcgcagtcc
     1681 acgctggggg agcgcagcga ctcgatgatc tggggaaagg cttccaggtc cggattgcgg
     1741 gcatcgatgc acacggccag atccggctgc ggctcgacgg cccgtgtggc gttcgcgatc
     1801 aggtggtgga gcaggtcgac ggggaagttg tcgatgcagt tgtagccgga atcctccagc
     1861 gctcgcaggg ccgtgctctt gcccgacccg gaccgaccgc tgatgacgat gagcttcatg
     1921 ccgcctgact gctgaagagc cccaccagtt cctgatagag ggcttcatcc gttgcgctgc
     1981 tgcgcaggcg tctgcgattt tccggatcgc cgaaaatccg cgccagggcg gccagcaggt
     2041 ccagatgggc cgtcgtttcc tcgggcggca cggcgagcac aaacagcaga tccaccggct
     2101 cgtcgtccat ggactcgtag tccacggcct ctcgcaggtg gagcagggcg ccggtgatct
     2161 gctcgcacgg aatcctgcag tgggggatgg cgacgccgtc gccgagcccc gtgctgccga
     2221 gccgctcgcg cgccatgagc tcgtcgaaca gcttgcgcgc ggacagatct ggatgatgct
     2281 tggccaccag gtctgcggcc gcctccaaag cgcgcttgcg cgaaggcgcc tcgacggaag
     2341 ctctcgtgca atccggtctg agtatggtgg tgatgtccgg catggccccc gctcagcgtc
     2401 cccggcgttg ttgtcggtcc agcagcttct ccttgtgctt gaggacctga cggtcgagtt
     2461 tgtccgtgag cccgtcgatt gccgcgtaga ggtcgtcgca atcgcagtcg gcgaacacct
     2521 cggtgccgct cgcgtgtatg gtcgcctcgg cgcgatggcg cagcttctcg acgctcagaa
     2581 ccacgtggac gttggtgatg tggtcgaagt ggagttccag tttctgcatt ttttcggtca
     2641 cgtagttccg cagcgcctcg gtgacgtcga tgtgatgacc ggagatgctc aactgcatgc
     2701 tggctccttg ttttgctgtt gatgtcgagt cgcgtcgtgt gccgtcatga atgatcaaac
     2761 cagacgcttg cgttcgttgg acggtggtat ggccatcgac tctcgatatt tggccacggt
     2821 gcgtctggcg acgttgatgt tctgctccgc cagtatcgta gcgatgcggc tgtcgctcaa
     2881 cggttttctt ggattttcat cggccgtcag cttcttgatg agcgcgcgga tggccgtgct
     2941 ggaaacctcg ccgccgctgc tcgtggacac gtggctggaa aagaaatatt tcagctcgaa
     3001 gatgccccgg ggcgtgtgca tgtacttgcg gctagtgacc cgggagatgg tcgattcgtg
     3061 catttccacg gcctcggcga tctcgttgag caccaggggc ttcatggctt cttccccgta
     3121 ttccaggaat ccccgctgat gttccactat gcgggtggcg accttgagca gggtctcgtt
     3181 gcggctctgc aggctcttga ggaaccatcg cgcttcctgc agatgatcgc gcaggaaggt
     3241 gttgtcggcg ctgttgtcgg cgcgcttgac gagcccggcg tagccggcgt tgattctcaa
     3301 gcccggcgtc gcgtcggggt tgagctccac cagccagcgc ccctctttcc tggataccat
     3361 gacgtcgggg acgacgtact ctgccgcctc ctgggccacc gtggtgcccg ggcgcggatt
     3421 gagcgtctga atgagcctca cgacctcgcc gagttcctct tcgccgagct tggtccgacg
     3481 tttgagggcg gcgaagtccc ggctggccag cagaggcagg tgctcgttga cgagggtcag
     3541 cgactccgcg agccacggcg tgcccgccgg cagctgctcg agctggatcc tcaggcactc
     3601 gcccaggtcc cgggcgccca ccccgatggg gtcgaaatgc tggatctgct tgagcacggc
     3661 catgacctcg gcgtgggcaa tctccgcctc ctcgtccacc acgccttcca gagacgtcac
     3721 cagctcttcc acgggcgtgg tcagcatccc gtccggactg atggtgtcga tgatcgccat
     3781 ggcgatcacc cgatcggagt cggacatggg cgtgaggttc agctgccaga gcagatggtc
     3841 ctgcagggtc tcctcgggcg cgttccgcgt ttcgaaatcc caggattcgg aatcgccggc
     3901 tgccggagcg ggagcaggcg cgggctggta gatctcgtcc cagctcgtgt cgaccggcag
     3961 atcgtcgata acgtcccctt ccagggtcgc atccacgccg tcgaggtcgt cgtcaccgtc
     4021 gctttcggcc cgatcggcct ctgcgggaga gcccgtcggc tcgtcgcccc cgtcctcctc
     4081 gtcgagttcg agcatcgggt tgccttccag ggcatcctga atttcctgac gcaggtccag
     4141 gcacgaaagc tgcagcagcc ggatggcctg ctgcagctgc ggcgtcatgg tgagctgctg
     4201 tccgagcttg agcgacagag tctgtttcat gcaaaccgag ggctctggtg agcggtcgtc
     4261 cttccgcgcg gcatggttgc cggttgttgt cctaacgggt caaaaggctt ggcgtgcctg
     4321 acgctcgccc ctggctgtgc ggccgcgcta tcgcggcctg gaactgaggg ctgatctcaa
     4381 ggagccaggc tcaaagagag aagctctcgc ccagatatac gtttttgacc ttctcgtctg
     4441 caagtatggc gccagaatcg ccttctgcaa tgatgtggcc ctcatgaacg atgtaggccc
     4501 ggtcacaaat gtccagggtg tcccgcacgt tgtgatcggt gatgagcacg ccgatgccgc
     4561 gggccgccag atcgcggatc tcctttttga tgtcgccgac ggaaatgggg tcgacgcccg
     4621 cgaacggctc gtcgagcagc atgaagctgg gctccatggc gagtgcccgg gcaatctcca
     4681 gccggcgtcg ctcgccgccg gagagccgct cgccgaggga ctcgcgcacc ccgcccaggt
     4741 gaaactcctc gatcagctga tcgagcatgg cctggcgttg ggtccggttc aggtcccgtc
     4801 tcagctccag gatggcccgc acgttgtcct ccgtgctgag ctttctgaag acgcttgctt
     4861 cctggggcag atagccgata cccgcccggg cgcgcacgtg catgggggtc cgggtgatcc
     4921 gctgctcgtc gagaaagata tcgccgccgt cggctcgcag caggccgacg atcatgtaga
     4981 aggtggtggt tttgccggca ccgttgggtc ccagcaggcc gatgatctcg ccggtgctga
     5041 cgtgcaggct gacgtcttcc accgcgacct tgttacggta ctgtttgcgt agagagcgaa
     5101 cttccagtgt gggcatgggg ccgtttaccc gccgtcgggt ttcttgcgag cgggctgaag
     5161 gatcatccgc accggcgttt cgctttccgc cttcgcatcc accttgccgg cgacgatgtc
     5221 gtactcgatg aggtccccgg tgatctcgtt gccttcctgg gtgagaaacg cgttgccgac
     5281 caggtcgatg cgctcgtcgc gcgtgtggta gatgatggtc gacgaccgag cgcgaaccac
     5341 ttcctcgtca acctcgatct gctgctggta gcgggcgggc tctcccttgg cggtgatgcg
     5401 tatcacctcc tgatcctcgt actccacggt catctcgtcg gccatgacgc gcagagtccc
     5461 ctggtccacc tgaacgtcgc cgcgatagat caccagcccc tgttcctgat ccatctccgc
     5521 gctgttggcg cgtatgtgaa tgggctggtc ggcgtcctcg ggcagagcga gcgcgcccgg
     5581 agcgagcagg acggcgccaa gcgtcacggc acgcagcagc ctacttgaac tgatccggca
     5641 gcagaatcgt gtgcacgcgg cgtcgatctg aggaggacag ctgtagcaat ccacgctgca
     5701 agtcaccttt gagtccggtg gccgtggtcc ggccggcatg ggtgtcgatc ataacatcgc
     5761 ggtcagtctc ggcgtactct cgctccggat acacatagag ggtctccgtg gtcaggcgta
     5821 tgtggcgacc gtcgggttcc cgctgctcta gctggacgtc ctcgcgcaga aaaaccacct
     5881 cttcgccttc gccgccacgc tcgggtccgc ccattcggcg cacgtagccg tgctccgact
     5941 gcacttgcca cggcgagcgc gtttcgctgt tgaccaccag gttcggcttg gtcagccgca
     6001 ccagctcgtc cgcctcgaac tgcctcagca atttcgacac cagccggtag tggacggttc
     6061 cgcttggcag gtactgggtg atgacggcgt cttcgacgta aagatccggc tcttcggcga
     6121 tgccggcggg cggggtctcc gcttctgtct ccggctccag gggctcgctc agcagaacga
     6181 ccccggccac cgcaacaccc aggatggccg tccacgtcga ccatcgttcc catttggagc
     6241 gcttgcgcat ggctcagccg gttacccgta gggccactcg ccccgggctt ccaggaaaag
     6301 ctgggcgatc tcccgcgccg cccctgcacc cccgggggcc agggtgacgt agtcggcggc
     6361 ggccgccgcg gccgggtgcg cgttgggaac gctgatggcc attccggcct gggagaacac
     6421 gccgaggtcc ggcagatcgt cgccgacata ggcagctcgc tcgagggtgg tgccgaccgt
     6481 ctcggccagg gccgccagga cggcggcctt gtccgcgacc ccctggtaga cgtgggcgat
     6541 gcccagctcg tcggctcgtc tcgtgacgat gctggaagac cggcccgtga tgatcgccac
     6601 cacgatgccg tgggaaagca ggagcttcaa agcggcgccg tcctggacat ggaaggcctt
     6661 cagctcgcga ccgtcgtcgc tgtagtagag cttgccgtcg gtgagcacgc cgtccacatc
     6721 gaaagccacc agctcgatat ccgccgcgcg ctcgagcacc ggcgccggga aagccatcag
     6781 cccagctgtg cctgagacac gagcagcagg ttgtagccga cccaggcgca cagcatgacc
     6841 gtgccctcga agcgggtgat cacgggctgg gacccgacgg cgtaggcgaa caccgcgagc
     6901 aggagcgtca gcgccagcat catgccgccg tcccgccaca gccagacgtt ctcgatgagg
     6961 atcgggtcca ccaggctggg gacggccagc accgccagga tgttcagaat gttggatccc
     7021 acgacgatgc cgatggcgat gtccgtgtgg cctttcagcg cggagcccac cgttgccgca
     7081 agctctggaa ggctggtgcc gatggcgacg attgtcaggc cgatgatgat gtcgttgacg
     7141 cccagccggt cggcgatgtc gctggcggcc cagaccagca cctcggcgct ggccagcagg
     7201 atcagcaacc cgatcagcag ccacacggct gcctggccgc gcgacatctg cggaagatcc
     7261 tccagctcct cggtgatgct ttctgccagg tcgcttcccg cggtcttttg actgcgcacc
     7321 aggcgccaca gaatcacgcc cagcccggcc agcagcagca ccgagtccaa tgcggacatc
     7381 tcgagatcga agagaagagc cagggccaga aaggtggcgc ccagcagcca gggcagctcg
     7441 gcgcgcagca cggtctggga aaagggcagc ggcacgatga tcgcggtgat gcccagcacc
     7501 aggccgatgt ttgcgatgtt cgagccgatg gcgttgccca cggcgagcag cgggttgcct
     7561 tccaccgcag ccgtcagggc cacgagtatc tcgggggccg acgtgccgag ggagaccacc
     7621 gtgaggccga tgagcatctt ggacacaccg aggtgcccgg cggtgcttgc cgcaccggcg
     7681 agaaagcgat cggcgctcca cagcagtgcc atgaagccaa cgatgagggc ggctatcgag
     7741 agcagcatga gctggcgccg gccgggttat ggggatcaat agtcaagttg gccactttag
     7801 tctcatcgcg cccgtcagcc aactccagcc cgcgatgtta tagttgcgcc cgtcttgctc
     7861 acgaccccag acgttcatga acggcgccga cgacaacctc attgagatcc gcaatctctc
     7921 tttcgtccgc ggtgaccggg tcattttcga tgatcttagc ctgtcggtgc cccgcggcaa
     7981 gatcactgcg atcatgggcc cgagcggcac gggcaagacg accctgctgc gaatcatcgg
     8041 aggccaggtg cgccccgaca gcggggtcgt gctcatcgat ggtcaggacg tctgcgggat
     8101 gagccgacgc gagctgttcg agctgcgtaa ggacatgggg atgctgttcc agtcgtccgc
     8161 gctgttcacc gacctgaacg tcttcgagaa cgtggcgttt cccctgcgcg ttcacaccga
     8221 tctccccgag gacaccgtac gcgacctcgt tctgatgaag ctgaacgcgg tagggctgcg
     8281 cggcgcgaga gatctcatgc cgagccagct ttccggtggc atgtcgcggc gggtcgccct
     8341 ggcacgtgcc gtcgcgctgg atccgaacct cctgatgtac gacgaaccct tcaccgggca
     8401 ggatccgatc gccatggggg tgctgctcag cctcatcgag agcctcaacg actcgctgaa
     8461 gacgacgagc ctcgtggttt ctcacgacat tcacgagacc atcagcattg ccgaccagat
     8521 ctatctcatc gccgacggca cgatcatggg atccggcacg cccgatactc tggcggtgga
     8581 cgactcgcca cgcgtccacc agttcatgca cggctcgccg gacggccccg tgccctttca
     8641 ctatcctgcc gacgagttcc tctacgacgt tctaggggac gtgaacgcgt gatccagttc
     8701 ctgcgcagct tcggtggccg cagcctggac cggatggcga gcctcggcag ggcgaccatc
     8761 atgtggttca cggcgctcgc ggggctcccg gggcccgggg acctgcgcct gctcgttcgc
     8821 cagatctacc acgtgggcgt gctgtccctc atcatcatcg ttctttcggc gttctccatc
     8881 ggtgcggtga tcgctctgca gtttcacacc cagctggcgc gtgtcggtgc cgaggactcg
     8941 gtgggtctcg ggctcgcgct ggtgctgctg cgcgagctcg ggccggtggt gaccgggctg
     9001 ctgttcgccg gtcgggcagg ttccgccatc acggcggaga tcgggctcat gaaaaccacc
     9061 gagcagctgt cgagcatgga gatgatgggc gtcgatccgc tgcgccggat cgtggcgccg
     9121 cggctttggg ccggggtcat cagcatgcct ttgctcaccg ccatcttcaa cg
//
LOCUS       DBNHOX010000221         2275 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_17388, whole genome shotgun sequence.
ACCESSION   DBNHOX010000221 DBNHOX010000000
VERSION     DBNHOX010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_17388"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..897)
                     /locus_tag="AC16UZ_04910"
     CDS             complement(127..897)
                     /locus_tag="AC16UZ_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003756 - protein disulfide isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HIR6246055.1"
                     /translation="MKLSFRTLWVGAVLACLAVIGHADEAAEADEVKDVTDAKGGQAL
                     VERLTALRPGIPIEQVRETPLAGIYALELKGGSFFYGTADGKYLFAGDMYELTDTSLV
                     NLAEAGRTAKRQALMAQVTAEEMVVFSPEGTTKASISVFTDVDCGYCQKLHLEVPKLN
                     EMGIEVRYLAYPRAGVGSQSYQKIVSAWCSADRNTAITRLKARQTIPTLTCSNPVASQ
                     LDLGREVGVTGTPAIVLEDGRLLPGYMPAEQLAEALGI"
     gene            complement(1021..1926)
                     /gene="xerD"
                     /locus_tag="AC16UZ_04915"
     CDS             complement(1021..1926)
                     /gene="xerD"
                     /locus_tag="AC16UZ_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR6246056.1"
                     /translation="MGEVLPKDEAARHTVDEYLDAVWLERGLSENTLAAYRRDLYQLA
                     DMLKRDPLTANDADLLECLALRHRQGYRASSAARWLSSVKGFYRHAAGRGRIGADPSA
                     HLEHPKMGRRLPGSLSEAEVRQLLAAPRLDDPIGLRDKAMLELLYASGLRISELVGLT
                     MSAVNARQGIVRIVGKGGKERLVPMGGEAMRWLERYLHEGRPLIKGSDATAAMFPSRR
                     GSQMTRQNFWHAVKRYAMKAGIHRSISPHTLRHAFATHLINHGADLRAVQMMLGHADL
                     STTQIYTHVAQARLKDLHRTHHPRG"
BASE COUNT          415 a          765 c          662 g          433 t
ORIGIN      
        1 tagaccacac attcctacct tgaggggttc caacgccaat gaccaagatg ctgcgacgag
       61 gcgccaagtc tactggcgcg ccgtcctgtg ctcaaaggta tcgacgggaa acttcgcccg
      121 aggggatcag atgcccagcg cttccgccag ctgctcggcc ggcatgtagc cgggcagcag
      181 ccgcccgtcc tcgagaacga tggccggtgt gcccgtcacg cccacttcgc gcccgaggtc
      241 gagctgggaa gcaaccgggt ttgagcaggt cagcgtggga atggtctgtc gcgccttcag
      301 cctggtaatg gccgtgttgc gatcggccga gcaccaggcc gacacgattt tctgatacga
      361 ctgagaaccc accccggccc gcggataggc caggtaccgg acctcgatgc ccatctcgtt
      421 gagcttgggc acctcgaggt gcagcttctg acagtaacca cagtccacgt ccgtgaacac
      481 gctgatggac gccttggttg tcccttccgg ggagaaaacc accatctcct ccgccgtcac
      541 ctgggccatc agggcctgcc gctttgccgt tcgccccgct tcggcgaggt tcaccaggct
      601 ggtgtcggtg agctcgtaca tgtctccggc gaacagatac ttaccatccg cggtaccgta
      661 gaaaaacgaa ccgcccttga gctcgagggc gtaaatgccg gcgagcgggg tttcgcgtac
      721 ctgctctatg ggtataccgg gacgcagcgc cgtcagccgc tcgacgagcg cctggcctcc
      781 tttggcgtcc gtgacgtcct tgacctcatc ggcctcggct gcctcgtcgg cgtgtccgat
      841 gacggccagg cacgctagca ctgccccgac ccagagggtc cgaaatgaca acttcattga
      901 tttcttcacg gtgcctcctg cacggccgga gcattagacc agcgagcgcg cccaaatatc
      961 cagcttaacc acattccttt acaaatagcc tgctccaata ctcaaacccg cgcttcgctc
     1021 tcatcctcgc gggtgatggg tccgatgcag gtccttcagc cgagcctgcg cgacgtgcgt
     1081 gtagatctgg gtcgtggaca ggtccgcatg tcccagcatc atctgcacgg ccctgaggtc
     1141 cgccccgtga ttgataaggt gggtggcaaa ggcatggcgc agcgtatgcg gcgagatgct
     1201 ccggtggatg cccgccttca tggcatagcg cttcaccgcg tgccagaagt tctgtcgggt
     1261 catttgcgag ccccggcggc tcggaaacat cgctgccgtg gcatcgctgc ccttgatgag
     1321 cggacggccc tcgtggagat aacgctcgag ccagcgcatt gcctccccac ccatgggcac
     1381 caggcgctcc ttgcccccct tcccgacgat tctcacgatc ccctgccgcg cgttcacggc
     1441 gctcatcgtg agacccacca gctcggatat cctcaacccc gaggcgtaga gaagttccag
     1501 catggccttg tccctgagcc cgatgggatc gtccaggcgc ggtgctgcca gcagctgacg
     1561 aacttccgcc tccgacaacg agcccggcag ccggcgtccc atcttcggat gctccaggtg
     1621 agcgctggga tccgcgccga tccgcccgcg cccggccgcg tgccggtaga agcctttcac
     1681 ggaggagagc cagcgggcgg cggagctggc gcggtaaccc tgacgatgcc gcagcgccag
     1741 gcactcgagc agatccgcgt cgttggctgt cagcggatcc cgcttcagca tgtcggcaag
     1801 ctggtagaga tcccgacgat aggcggccag cgtgttctcg ctcaagcccc gctcgagcca
     1861 gaccgcgtcg agatattcgt ccaccgtatg gcgggccgcc tcatccttcg gcagaacctc
     1921 gcccatttcg tctccttggg cacccatcac ggacactttg tgacgtagtt ggtcctcagt
     1981 caaccgacat aaaaaccagt ggatagttat tcaactacat gaaacaaaag gatttttaat
     2041 cgattttgaa cggaatccag atagctggca cggtagctgc attggcttat acgggcgcga
     2101 tccacccacc cagctgtccc ctggagtgat cgtgacccac gctgaaggaa gccggccccc
     2161 tctccaccgg tttccgaaga cggccagctt gccggggcct ccccccaact cccccaaccc
     2221 ggccagctgg cttcattttt tttaagccgc cgggcggtga aacgacgggt tagac
//
LOCUS       DBNHOX010000222         6141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1560837, whole genome shotgun sequence.
ACCESSION   DBNHOX010000222 DBNHOX010000000
VERSION     DBNHOX010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1560837"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="AC16UZ_04920"
     CDS             complement(<1..662)
                     /locus_tag="AC16UZ_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR6246057.1"
                     /translation="MEDGLDRQASRELTRLLPALGFLRPYVRQVVFASIALVVTAGVT
                     LSIGQGMRLVIDEGLGSGSAAVLVQSISIFAVLMVVLTAGTFLRFYFVSWVGERVSAD
                     IRQAVFAHLIRLHPGFFETNLPTEIQSRITTDTTVLQTVIGSSVSIALRNVLMFVGGL
                     VLLFVTNPKLSLIVVASVPFVVAPVILFGRRVRALSRDSQDTLAEVGSFAGESLRNIK
                     IV"
     gene            796..1449
                     /locus_tag="AC16UZ_04925"
     CDS             796..1449
                     /locus_tag="AC16UZ_04925"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6246058.1"
                     /translation="MTDLLDCVEIDPGGTAGSVIWLHGLGADAHDFEPVVPLLPLNRP
                     LRFVFPNAPVRPVTINAGMEMRAWYDIDPGNPLSGDSDIQSSAKAIAGLVEREHDRGV
                     PYDGITLAGFSQGGVIALELGLSFPHRLAGIMALSTYVPDHEHLVDRIGFANVDAPIF
                     MAHGIGDPMIPITRAITSREALLAMNYEVEWHEYGMGHQVCPEEIADIGTWLNTIYR"
     gene            1547..2323
                     /locus_tag="AC16UZ_04930"
     CDS             1547..2323
                     /locus_tag="AC16UZ_04930"
                     /inference="COORDINATES: protein motif:HMM:NF018649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR6246059.1"
                     /translation="MGLFELYGVAALTVWVMVTTLWAVSVPTKDASIIDMFWGPLFVA
                     IAWVLLPVYGGGVQAKPYLVMLLVTLWGLRLAYHLIGRNLAAGEDVRYARWRASGGPK
                     WWLRTYHRIFLLQGFLALLVATPILASFYAPRDLTLLNLAGVLIWAAGFVFELVADVQ
                     LSRFKADPANRGQLLNTGLWGLSRHPNYFGDALQWWGLSIVALSGAAWWALLGPVVMT
                     MIFLVLTADILEQGMSRRHPDYEEYVSNTPKFVPRLPGRD"
     gene            2475..3422
                     /gene="argC"
                     /locus_tag="AC16UZ_04935"
     CDS             2475..3422
                     /gene="argC"
                     /locus_tag="AC16UZ_04935"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR6246060.1"
                     /translation="MIRVFIDGQAGTTGLEIVQRLAARDDVELLEIDPAERKSPAARQ
                     RLFDSADVAVLCLPDDAAREAAVLADGRCRIIDASTAHRIQEGWCYGLPELGSNQRQA
                     LAAAERVANPGCYPQGFILMIRPLIGAGLLDPEQPLAVQALSGYSGGGRTLIEARRAF
                     GSQEAERRNTEPYALSLKHKHVPEMQAYSGTKIAPLFTPSVGQFYQGMLVGVPLFCSQ
                     LRKGADYDGIYHILEEAYALEPFVDVLEPGAETALTDGFLNATACNGTNRLEIMLFGH
                     SEQLLLMARYDNLGKGAAGAAVQNLNLMMGIDETTGLTS"
     gene            3419..4738
                     /locus_tag="AC16UZ_04940"
     CDS             3419..4738
                     /locus_tag="AC16UZ_04940"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF024301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004069 - L-aspartate:2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase type Ic"
                     /protein_id="HIR6246061.1"
                     /translation="MTTARAIAALSDPELAALKAELSRELELQAGNRLSLDLTRGKPA
                     ADQLDLSAGLDDAIAGNYIASDGTDSRNYGALTGLGEARELGAEIMEADPGDIICWGN
                     SSLQLMHICVDLALNQGLWGDGRRWRRDANPKVLTPVPGYDRHFTICEAAGIDMVNVP
                     MTDTGPDMDAVRRATEDASVKGIWCVPKYSNPSGCIYSPECVDALARLPADAAADDFL
                     ILWDNAYAVHDFEFPRAPLASLLDLARKHGTADHVIMFASTSKITYPSAGLAFLAADA
                     KVRKTVEKRLGTASIGPDKVNQLRHARFLGGRVEAHMAGHAALVKPKFDLVESKLREG
                     LAGLEIASWTTPAGGYFVSLDTRPGLAKKVGELAAQAGLTITKPGATFPYGRDPEDRN
                     LRIAPTFASLADLNVAMELLVVCVKLAAVGAEIHQRTDTGGAQKAHE"
     gene            4731..5921
                     /locus_tag="AC16UZ_04945"
     CDS             4731..5921
                     /locus_tag="AC16UZ_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009867821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6246062.1"
                     /translation="MSKWRIEDFPVAPTEGKDRFHDFALPDPLMHAIADLEFEYCTPI
                     QSAVLPWSLADYDVTGQAQTGTGKTAAFLVTIIARLWEYPPAAAPKIGSPRSLVLAPT
                     RELALQIESDAKDLTKYIDLGVECVVGGMDFQKQLDRIKRRPVDVLIATPGRLLDFIS
                     RRHLSLRDVEVLVIDEADRMLDMGFVPDVRRIVYQTPHKRNRQTLFFSATFNDDVMRL
                     ANSWTIDPEHVAITPESVATDTVTQKFWLVAADKKASLLAEFLTRHAPERALIFTNRR
                     DQTHQLHAFLNKRNIRCEALAGDVPQRKRLSTLSRFKDGDIPYVVATDVAGRGIHVEA
                     ISHVINFDLPEDPEDYVHRIGRTGRAGAEGVSISFVSEDDAFNLPSIEEYLGEAVVCT
                     QPSF"
BASE COUNT         1129 a         2025 c         1958 g         1029 t
ORIGIN      
        1 tgaacgatct tgatgttgcg cagcgactcg ccggcaaagc ttcccacctc ggcaagggtg
       61 tcctggctgt cgcgcgacag cgcccgcacg cgccggccga acaggatcac cggggccacc
      121 acgaagggca cgctggccac cacgatcagc gagagcttcg gattggtcac gaacaggagc
      181 accagcccgc cgacgaacat caggacattg cgcagggcga tggagacgga cgatccgatg
      241 acggtctgca gcacggtcgt gtcggtggtg atccgcgact ggatctcggt gggcagattc
      301 gtctcgaaga aacctggatg aaggcggatg agatgagcga agaccgcctg gcggatgtcc
      361 gcgctgacgc gctctcccac ccaggagacg aagtagaaac gcagaaacgt cccggccgtg
      421 agaaccacca tgagaacggc gaaaatcgat atgctctgca cgagcacggc ggccgatccg
      481 ctgcccaggc cttcgtcgat gaccaggcgc atcccctggc ctatggaaag cgtcacgccc
      541 gccgtcacga cgagggcaat gctcgcgaat accacctggc gaacatacgg gcgcagaaag
      601 cccagagcgg gcagcagccg cgtcagctcc cgtgatgcct gtctgtcgag tccgtcctcc
      661 atggccgtag tatctcacct gcagggccgc gaacgtaggg ccgccgcgtt gaccgaccct
      721 ggcgagagaa acggagattg ctttcccccc gagatcttgt ttagctgccg cggagctcgc
      781 ccgtcgggaa atacggtgac tgatctgctg gactgcgtcg agatcgaccc gggcggaacc
      841 gccggttcgg tgatatggct gcacgggctg ggcgccgacg cgcacgactt cgaaccagtg
      901 gtgcccctgc tgccgctgaa ccggccgctg cgcttcgtgt tccccaacgc gcccgttcgc
      961 ccggtcacca tcaatgccgg catggaaatg cgcgcctggt acgacatcga tcccgggaac
     1021 ccgctgtccg gcgattcgga cattcaatcc tcggccaagg cgatcgcggg gctggtagag
     1081 cgcgagcacg accgcggcgt gccatacgat ggcatcacgc tcgcggggtt ttcccagggg
     1141 ggcgtcatcg cgctggagct gggcctgtcg ttcccccaca ggctggccgg gatcatggca
     1201 ctctccacct acgtgcccga tcacgagcac ctggtggatc ggatcgggtt cgccaacgtg
     1261 gacgccccca tcttcatggc acacggcatt ggagatccga tgatccccat cacccgtgcg
     1321 atcacctcgc gggaggcgct gctggcgatg aactatgagg tcgagtggca cgagtacggc
     1381 atgggccacc aggtgtgccc ggaggagatc gccgacatcg gcacgtggct gaacaccatc
     1441 taccgctgat gcgtcgggaa agagcgagaa ctcgggtggc cctaaggcgc acccgcgaat
     1501 tcagtaacgg ccgcgaggat cgagcggaga tagagtggag atagacatgg gactgttcga
     1561 gctgtacggg gtcgccgcac tgaccgtatg ggtgatggtc acgacactgt gggccgtcag
     1621 cgtgccgacc aaggacgcaa gcatcatcga catgttctgg gggccgctgt tcgtggccat
     1681 agcctgggtg ctgctgcccg tctacggtgg cggtgtgcag gccaagcctt acctggtcat
     1741 gctgctcgtc accctgtggg gccttcggct ggcctatcac ctgatcggtc gcaatctcgc
     1801 cgccggcgag gacgtccgct atgcccgatg gcgagcgtca ggcggaccga agtggtggct
     1861 gagaacctac caccgaatct tcctcctgca gggatttctc gccctgctcg tcgccacgcc
     1921 catcctcgcc agcttctacg caccccggga cctgacgctg ctgaacctcg ccggcgtcct
     1981 gatctgggcc gccggtttcg tcttcgagct cgtcgccgac gttcagctca gccgattcaa
     2041 ggcagatcct gccaaccggg gccagctgct caacacgggc ctgtggggcc tgtcccgcca
     2101 ccccaactac ttcggcgacg cgttgcagtg gtggggtctg tccatcgtcg cgctgtcggg
     2161 cgccgcgtgg tgggcgctgc tcggccctgt ggtcatgacc atgatatttc tcgtgctcac
     2221 cgccgacatt ctcgagcaag ggatgtctcg ccggcatccc gattacgagg agtacgtatc
     2281 caacacgccc aagttcgttc cccggctccc cggccgcgat tgacccgggg atccgcgggg
     2341 ggcacagacg ggcaaacggg tgccggaaca gccgaggggc catcttttcc gtcgcgccgc
     2401 tgtgacatcg ggccgatatt gcctaaaatc gcgcgcccct aagaaacaac cccgcgggga
     2461 aggacctcgg caccatgatc agggtattca tcgacggaca ggccggcacc acgggcctcg
     2521 agatcgtcca gcgcctggcg gcgcgcgacg acgtcgagtt gctggagatc gatcccgccg
     2581 agcgcaaatc acccgcggcc cgacagcggc tgttcgactc tgccgacgtt gccgtcctgt
     2641 gcctgcccga cgacgcggcg cgcgaggccg cagttctggc ggacggccgc tgccgcatca
     2701 tcgacgccag cacggcccac cggattcagg agggatggtg ctacggtctg cctgagctag
     2761 gatccaacca acgccaggcc cttgccgcgg cagaaagggt cgccaatccc ggttgctatc
     2821 cccagggctt catcctcatg atccgccccc tcatcggcgc aggcctgctg gaccctgagc
     2881 agcccctggc cgtgcaggcg ctttccggct attccggggg agggcgcacg ctcatcgagg
     2941 ctcgcagggc cttcggttcc caagaggccg agcgccgcaa taccgagccc tatgctttgt
     3001 cgctcaagca caagcacgtc cccgagatgc aggcttactc gggaacgaag atcgccccgc
     3061 tgtttacgcc gtcggtgggc cagttctacc agggcatgct ggttggggtc ccgttgtttt
     3121 gcagccaact ccgcaagggc gcagactatg acggcattta tcacatcctc gaggaagcct
     3181 acgccttgga acctttcgtc gacgtgcttg agcccggtgc ggagaccgcc ctgacggacg
     3241 gttttctcaa cgccaccgcc tgcaacggca ccaaccggct cgagatcatg ctgttcgggc
     3301 actcggagca gctgctgctg atggcgcggt acgacaatct cggcaaaggg gccgccgggg
     3361 ccgcggttca gaacctgaac ctcatgatgg ggatcgacga gaccacgggc ctgacatcat
     3421 gacgacggca cgagccatcg ccgcgctttc cgaccccgag ctcgcagcgc tgaaggccga
     3481 gctgtcccgg gagctggaac tccaggccgg gaaccgtctg tcgctggatc tcacgcgcgg
     3541 gaagccggcc gcggatcagc tcgacctgag cgcgggcctg gacgatgcca tcgcgggcaa
     3601 ctacatcgct tcggacggaa cggactcgcg caactacggc gctctcactg gcctgggtga
     3661 agccagggaa ctcggcgcag agatcatgga ggccgacccc ggggacatca tctgctgggg
     3721 gaactcgagc ctgcagctca tgcacatctg cgtcgatctg gctctgaatc agggcctgtg
     3781 gggcgacggg aggcgatggc ggcgcgacgc aaatcccaag gtgctgaccc cggttcccgg
     3841 ctacgaccgc cacttcacca tctgcgaagc ggccggtatc gacatggtca acgtgcccat
     3901 gacggatacg ggtccggaca tggacgccgt tcgaagggca accgaggatg cgtccgtgaa
     3961 gggcatctgg tgcgtgccta agtattccaa tccctcgggg tgcatctact caccggagtg
     4021 cgtggacgcg ctggcccggc tgccggcgga tgcggcggcg gacgattttc tgatcctgtg
     4081 ggacaatgct tacgccgtgc acgacttcga atttccgcgg gcgcctctgg cctccctgct
     4141 cgatctcgcg cgcaagcatg gcacggccga tcacgtgatc atgttcgcct ccacatccaa
     4201 gatcacctac cctagcgcgg gtctggcctt cctggctgcc gacgccaagg tgcgaaagac
     4261 ggtggagaag cgcctcggca cggcgagcat aggcccggac aaggtcaatc agctgcgcca
     4321 cgcgcggttc ctcggcggcc gggtggaggc ccacatggcc gggcacgcgg cgctggtgaa
     4381 gccgaaattc gacctggtcg aaagcaagtt gcgcgaaggt ctggcgggct tggaaatcgc
     4441 aagctggacc acgccggcgg gcggatattt cgtgtctctg gacacacgac ccggcctggc
     4501 gaaaaaggtg ggcgagctgg cagcccaggc cggattgacg atcaccaagc ccggcgcaac
     4561 gtttccctac ggccgggatc ccgaggaccg caacctgcgg atcgcgccca cgtttgcaag
     4621 cctcgcggat ctcaacgtcg cgatggagtt gctcgtcgtt tgcgtcaagc tcgccgccgt
     4681 cggcgcggag atccatcaac ggaccgacac aggaggagca caaaaagctc atgagtaagt
     4741 ggcgcatcga ggatttccca gtagccccaa ccgagggcaa ggatcgcttt cacgatttcg
     4801 ccctgccaga tcctctgatg cacgccatcg cagacctcga gttcgagtac tgcacgccca
     4861 ttcagagcgc ggtgctgccg tggagcctcg ccgactacga cgtcaccggc caggcccaga
     4921 ccggaaccgg aaagacggcg gcgttcctgg tcacgatcat tgccaggttg tgggagtacc
     4981 ctcccgccgc ggcgcccaag atcggttcgc ctcgttcgct ggtgctggca ccgacccggg
     5041 aactcgctct gcagatcgaa agcgatgcca aggacctgac caagtacatc gacctcggtg
     5101 tggagtgcgt ggtcggcggc atggactttc agaagcagct ggaccgcatc aagcggcgtc
     5161 ccgtcgacgt gctgatcgcc acccccggcc ggctgctcga cttcatcagc cgccgtcacc
     5221 tgagcctgcg ggacgtggag gtcctggtca tcgacgaggc cgaccgaatg ctcgacatgg
     5281 gcttcgttcc cgacgtccgc cgcatcgtct atcagacgcc ccacaaacgg aaccggcaga
     5341 cgctgttctt cagtgcaacc ttcaatgacg acgtgatgcg gctggccaat tcctggacca
     5401 tcgatccaga gcacgtcgcc atcaccccgg aatccgtggc gacggatacg gtcacccaga
     5461 agttctggct ggtcgccgcc gacaaaaagg cttcgctgct cgcggagttc ctcaccaggc
     5521 atgcgccgga gcgggctctg atattcacga accgccgtga tcagacccac cagctccacg
     5581 cgttcctcaa caagcgcaat atccgctgcg aggccctggc aggcgacgtg ccccagcgaa
     5641 aacgcctttc gacgctgtcg cggttcaagg acggcgacat cccctacgtc gtggcaacgg
     5701 acgtggccgg caggggcatc cacgtcgagg cgataagcca cgtcatcaac ttcgacctcc
     5761 cggaagatcc cgaggactac gtgcatcgca tcggccgtac aggtcgcgcc ggggcagagg
     5821 gtgtgagcat cagcttcgtc tcggaagacg acgcgttcaa cctgcccagc atcgaggaat
     5881 atcttggtga ggccgtggtc tgcacccagc cgagcttctg agcgggcaac ggtaccgccc
     5941 aagccgcagg ggaccccggt gccgtgaacc aaggccaccg tctaaccagg gtaggcgtcg
     6001 tccagctatg cgcgacgccg gacgtcacgg ccaacatgat tgccgtcgag cgtctcgtcc
     6061 cccaggcctg cgatgcgggc gccgaggtcg tgctgctgcc cgaggccttc gcatttctcg
     6121 gaccggaaaa ggcaaagcgc g
//
LOCUS       DBNHOX010000223         1923 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1111225, whole genome shotgun sequence.
ACCESSION   DBNHOX010000223 DBNHOX010000000
VERSION     DBNHOX010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1111225"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="AC16UZ_04950"
     CDS             <1..443
                     /locus_tag="AC16UZ_04950"
                     /inference="COORDINATES: protein motif:HMM:NF019464.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helical membrane plugin domain-containing
                     protein"
                     /protein_id="HIR6246063.1"
                     /translation="EKAMLQLNSISELAGDASHLAEPVMESLTRRMAELESRGYVDFA
                     RSGWGIVDRVVTSYTEEDVEALGENIVLILDTVRQMTQPEVMSMLRRTLDTVGDTDES
                     AEPPSLLGLFKEIRRPETRRGLARVLTMLRSVGDRNVSPDPGSR"
     gene            466..780
                     /locus_tag="AC16UZ_04955"
     CDS             466..780
                     /locus_tag="AC16UZ_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HIR6246064.1"
                     /translation="MPVATIAGHDVHVDVEGFMTEFEEWSEEIAGQLAANIGIEMTDD
                     HWKVVRFVRDDYLAQGETPTLRRVTSESGFPTKELFLLFPKKPAKKMSYIAGVPKPAG
                     CV"
     gene            795..1325
                     /locus_tag="AC16UZ_04960"
     CDS             795..1325
                     /locus_tag="AC16UZ_04960"
                     /inference="COORDINATES: protein motif:HMM:NF025066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HIR6246065.1"
                     /translation="MTATREAPSLVPDFGDEVQRKICFICSKGNLDMAYPALIMASGA
                     LTEGAEVDIFFTFWGLDMINQDTVDKLQFTMLGNTAMHMPGSSAHLPQGLAGLPGMTA
                     FSTRMLKKQISAQQVPDVREFITLLHDMGARLWACKMSADMGGLEKEDLLDEVEGIIN
                     VNEFIELSEDAQVVFI"
     gene            1432..1824
                     /locus_tag="AC16UZ_04965"
     CDS             1432..1824
                     /locus_tag="AC16UZ_04965"
                     /inference="COORDINATES: protein motif:HMM:NF015581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR6246066.1"
                     /translation="MSYHFSVTVHDDFEGVVSRTREALAEEGFGIVSEIDMAGTLKEK
                     LDYDMEPYLILGACNPPFALRAVQADPAIGTLLPCNVVVRQSGDAVVVDFMDPQAVLE
                     LVHAEGVRNVADDVRTRMEQVRDTLARG"
BASE COUNT          361 a          594 c          634 g          334 t
ORIGIN      
        1 tcgagaaggc gatgctccag ctgaacagca tctcggagct ggccggagat gcctcgcatc
       61 tcgcggagcc ggtgatggag tcgctgaccc ggcgcatggc cgagctcgag agccgcgggt
      121 acgtcgactt cgcccgcagc ggctggggca tcgtcgaccg ggtcgtcacc agctacaccg
      181 aagaggacgt cgaggcgctg ggcgaaaaca tcgtgttgat cctcgacacg gtcaggcaga
      241 tgacccagcc cgaggtgatg tccatgctgc gcaggacgct cgacacggtg ggtgacacag
      301 acgaatccgc cgagccgccg tcgctgctcg gcctgttcaa ggagatacgc cggccggaga
      361 cccgccgcgg cctggcgcgc gtgttgacca tgttgcggtc ggtgggggac cggaatgtct
      421 cccccgaccc cggttccaga tgatcacgag agaaagagac cactgatgcc cgttgccacc
      481 attgccggcc acgacgttca cgtcgatgtc gaaggcttca tgacggagtt cgaggaatgg
      541 agcgaagaga tcgccggaca gctcgccgcc aacatcggca tcgagatgac cgacgaccac
      601 tggaaggtcg ttcgcttcgt gcgagacgac tacttggctc agggggagac gccgaccctg
      661 cgcagggtga cctccgagtc ggggtttccc accaaggagc ttttcctgct gtttccgaag
      721 aagcctgcca agaagatgtc gtacatcgcc ggtgtgccga agccggccgg atgcgtctga
      781 gaggagtcgg tcacatgact gcaacgcgcg aagctccgag cctggtcccc gacttcggtg
      841 acgaggtcca acgcaagatc tgtttcatct gctcgaaggg caacctcgac atggcgtacc
      901 cggctctgat catggccagt ggggcgctga ccgaaggtgc ggaagtcgac atcttcttca
      961 ccttctgggg tttggacatg atcaaccagg acacggtgga caaactccag ttcacgatgc
     1021 tcggcaacac cgcgatgcac atgcccggct cctcggctca tctcccgcag ggcctggcgg
     1081 ggctccccgg aatgacggcg ttttccactc gcatgctgaa aaagcagatc tccgcccagc
     1141 aggttccgga tgtccgcgag ttcataacgc tgcttcacga catgggagcg aggctgtggg
     1201 cctgcaagat gtcggcggac atgggaggcc tcgagaagga ggatctcctc gacgaggtcg
     1261 agggcatcat caatgtcaac gagttcatcg agctgtccga agatgcgcag gtggtcttca
     1321 tctgattgac tgggttcggt gcggcccggg ccgatctacc ctggcgggtg atggtccggg
     1381 ccgttgaact ggccgccagg cgttggcacc accgaaagga aacgatctgt catgtcctat
     1441 cacttcagcg tcaccgtcca cgacgacttc gaaggagtcg tcagccggac ccgggaagcc
     1501 ctggccgagg aagggttcgg aatcgtgtcg gagatcgaca tggcggggac gctgaaagag
     1561 aagctggact acgacatgga gccgtatctg attctcggtg cctgcaaccc gccattcgcc
     1621 ctgcgggcgg tccaggcgga tccggccatc gggaccctgc tgccgtgcaa cgtggtcgtg
     1681 aggcagtccg gcgacgccgt ggtggtcgac ttcatggatc cccaggcggt gctcgaactc
     1741 gtccatgccg agggagtgcg caacgtggcc gacgacgttc gcacccggat ggagcaggtc
     1801 cgcgacacgc tcgcccgcgg ctgagcggag tcgcaccttc cctctcgccg gcctgcgcca
     1861 cgcctctttc ccggcgccgc gagtgtcggg cgcgcaaccg gtgaaacgcc gacactcggt
     1921 ccg
//
LOCUS       DBNHOX010000224         2299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1111245, whole genome shotgun sequence.
ACCESSION   DBNHOX010000224 DBNHOX010000000
VERSION     DBNHOX010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1111245"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="AC16UZ_04970"
     CDS             complement(<1..278)
                     /locus_tag="AC16UZ_04970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6246067.1"
                     /translation="MQPVERHVDVATPDGSMNTFIAYPQGGGPFPVVVFYMDAPGKRE
                     ELHDMARRLASVGYFVVLPNLYYRKSRDFWLQERTEARLAHMFSLMATL"
     gene            complement(280..1086)
                     /locus_tag="AC16UZ_04975"
     CDS             complement(280..1086)
                     /locus_tag="AC16UZ_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246068.1"
                     /translation="MADLKGKVAVVTGASRGVGRGVAEGLAEAGATVYVTGRSESHDN
                     PPQERTIQATAASVDALGGKGIAVRVDHNEDAEVKALFERVMSEQGRLDVLVNNVYKI
                     PDPPVFDGGFWEHPVSVWDDQCGVGLRGYYVASVFAAPMMVARRSGLIVNISSRGGDQ
                     YVFSTSYGVGKAGVDRMARDMAHELRGYHVAAVSLRPSSVKTEFILDAVASGAVKLDM
                     AKAQSPRFTGRCVAALAMDPDLMEKSGGVYMVADLAAEYRFTDPDRVEAS"
     gene            1218..1628
                     /gene="fur"
                     /locus_tag="AC16UZ_04980"
     CDS             1218..1628
                     /gene="fur"
                     /locus_tag="AC16UZ_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HIR6246069.1"
                     /translation="MPGSDIKRAGLKVTLPRLKVLEVLESAEPHHLSAEDVYKSLIDS
                     DDSVGLATVYRVLTQFETAGIVSRHNFDDGHSVYELAGDEHHDHMVDMDTGQVIEFVN
                     ERIEALQREIAQEHGFELIDHELVLYVRKQGKSE"
     gene            1737..1961
                     /locus_tag="AC16UZ_04985"
     CDS             1737..1961
                     /locus_tag="AC16UZ_04985"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR6246070.1"
                     /translation="MSLRDLSPGDMARVSGYREDDDYAAQLMRLGLIPGTEIRVQRRA
                     PLGDPMEIRFRGFSLALRPSEADALMLELI"
     gene            1958..>2299
                     /locus_tag="AC16UZ_04990"
     CDS             1958..>2299
                     /locus_tag="AC16UZ_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019485787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HIR6246071.1"
                     /translation="MTTVALVGNPNCGKTTLFNALTGTRQRVGNWPGVTVERKTGSYF
                     HGDGSVDVVDLPGTYSVHPVGDAVDERIAREFISAAEPSGPDVVVNVVDASSLARGLY
                     LTTELLQMGKPV"
BASE COUNT          403 a          780 c          726 g          390 t
ORIGIN      
        1 agcgtggcca tcagcgagaa catgtgggcc agcctggcct cggtgcgttc ctgcagccag
       61 aagtcgcggc tcttgcggta gtacaggttg ggcagcacga cgaagtagcc caccgacgcc
      121 agccgccgcg ccatgtcgtg cagctcctcg cgcttgcccg gcgcgtccat gtagaacacc
      181 accacgggga acggaccgcc cccctggggg tacgcgatga acgtgttcat ggacccgtcg
      241 ggcgtggcaa cgtccacgtg acgttccacc ggctgcacct cagctcgcct ccacgcggtc
      301 cgggtcggtg aaccggtact cggccgcgag gtcggccacc atgtagacgc cgcccgactt
      361 ctccatgaga tccgggtcca tggccagcgc ggccacgcac cgcccggtga accgcggcga
      421 ctgcgccttg gccatgtcga gcttgaccgc gccgctggcc accgcgtcca gaatgaattc
      481 ggtcttcacc gaggagggtc gcagggacac ggcagcgaca tggtagccgc gcagctcgtg
      541 ggccatgtcc cgagccatgc gatcgacccc cgccttgccc acgccgtagg aggtgctgaa
      601 cacgtactgg tcgccgcccc gtgaggagat gttcacgatg agaccgctgc gccgggcgac
      661 catcatcggc gcggcgaaca cggaggccac gtagtagcct cgaagcccca ccccgcactg
      721 atcgtcccag acggataccg ggtgctccca gaagccgcca tcgaaaaccg gcggatcagg
      781 aatcttgtag acgttgttga ccaggacgtc gagcctgccc tgctcgctca tgacgcgctc
      841 gaagagagct ttgacctcgg cgtcttcgtt gtgatctacc cgcacggcaa ttcccttgcc
      901 gccaagggca tcgacgctcg ccgcggtcgc ctgaatggtg cgctcctgag gcgggttgtc
      961 gtggctctcg ctgcgcccgg tgacgtagac ggtggcgccg gcctcggcca ggccttcggc
     1021 tacgccccgg cctaccccgc ggctcgcacc ggtaaccact gccaccttgc ctttcagatc
     1081 tgccacgtga tcgtcctccc gatgtcgtta ccccgtgccg acggcgattg taacccagcc
     1141 actccacgcc ccatggtcac gggtggacgg tcagccctgc atgggctagt gtccccaact
     1201 accgttgggg aaagccgttg cccggatccg acatcaaacg tgccggcctg aaggtcacgc
     1261 ttccgcgcct gaaggtgctg gaggttctgg agagcgccga gccgcaccat ttgagcgccg
     1321 aagacgtcta caagagtctc atcgattcag acgattccgt gggtctggcc accgtttacc
     1381 gggtgctgac ccagttcgag accgccggca tcgtatcccg ccacaacttc gatgacggcc
     1441 actcggtgta cgagctggcc ggcgacgagc atcacgatca catggtggac atggacaccg
     1501 gtcaggtcat cgagttcgtc aacgagcgga tcgaggcgct gcagcgggag atcgcccagg
     1561 agcacggatt cgagctcatc gaccacgagc tcgtacttta cgtgcgcaag cagggaaagt
     1621 cggagtaagc ggacgagccc tctgccgccc cacctgctgc caattgccaa tgagaatgat
     1681 ttttatttaa atggcgggcc gttccccacg gcccgaaccg gaggccaccg ttgtccgtgt
     1741 cactacgcga tctttcaccc ggagatatgg cccgcgtcag cggctaccgg gaggatgacg
     1801 actacgcagc ccagctgatg cggctcggcc tcattccggg caccgagatc agagtgcagc
     1861 gccgtgctcc cctgggggac ccgatggaga tccgcttccg cggcttctcc ctggcattgc
     1921 ggccatccga ggccgacgcg ctgatgctgg aactgatatg accaccgtcg ccctggtggg
     1981 caatcccaac tgcggcaaga cgacgctgtt caacgcgttg acgggcacgc gtcagcgcgt
     2041 aggcaactgg ccgggcgtta cggtggagcg caaaacgggc agctacttcc acggcgacgg
     2101 ttccgtggac gtggtcgacc tgcccggcac ctacagcgtg catcccgtcg gagacgcggt
     2161 ggacgagcgc atcgcccggg agttcatcag cgccgctgag ccatcgggcc cggatgtcgt
     2221 cgtcaacgtg gtggacgcat cgagtctcgc ccgcgggctc tacctgacca ccgagctgct
     2281 gcagatgggc aagccggtg
//
LOCUS       DBNHOX010000225         3178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1111393, whole genome shotgun sequence.
ACCESSION   DBNHOX010000225 DBNHOX010000000
VERSION     DBNHOX010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1111393"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="AC16UZ_04995"
     CDS             <1..375
                     /locus_tag="AC16UZ_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6246072.1"
                     /translation="RRVLELCRLRIAAIHDCAQEWGIRDAEVALTQPQMEALRSGDLS
                     NFDPPERAALELAERVPYQHHQLTDEEVVHAEGLLGGPGTVSLLTALAFFDVVCRLRL
                     TLELDEESALLASPPLADGAMV"
     gene            391..588
                     /locus_tag="AC16UZ_05000"
     CDS             391..588
                     /locus_tag="AC16UZ_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246073.1"
                     /translation="MSTKPRLAPAIPGSEADFASLMAQAPNSTALFFELYAEFWQNGV
                     VAEEIKEMTRMRNARITDCGY"
     gene            965..1450
                     /gene="ribH"
                     /locus_tag="AC16UZ_05005"
     CDS             965..1450
                     /gene="ribH"
                     /locus_tag="AC16UZ_05005"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR6246074.1"
                     /translation="MAQTAIDISQLPMIENAGRVAILKSKWYPELVDSMARCCEGVLR
                     DRGIDRVEVHTLPGSLELPLAAADLLAADSGGDIDAIVCFGIILKGETLHFEMITNEC
                     MRGLGEVMQRYRRPVIVEILPVLEMAHAESRAADDEFNKGIEAAAAAIEMIAWRRAVG
                     A"
     gene            complement(1447..1923)
                     /gene="smpB"
                     /locus_tag="AC16UZ_05010"
     CDS             complement(1447..1923)
                     /gene="smpB"
                     /locus_tag="AC16UZ_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR6246075.1"
                     /translation="MNSKTGKPAPKTIALNKRAKRDYHFEQTFEAGLVLEGWEVKSLR
                     AGKAQLVDSYVLVRDGEAWLLGANISPLPSASTHVVADPQRTRKLLLHTKEIAQIFTA
                     TRQKGYACIATALYWKGNRVKCELALGKGKKLHDKRATERERDWNRQKQRIMKQAG"
     gene            2018..>3178
                     /locus_tag="AC16UZ_05015"
     CDS             2018..>3178
                     /locus_tag="AC16UZ_05015"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR6246076.1"
                     /translation="MAPATESRHGMWSSRWLFVLAAAGSAVGLGNIWKFPYIAGENGG
                     GAFVIVYLVCIALVGVPIMMAEVMMGREGRQSPINTMRTLTEAHGRSPLWVLIGWMGV
                     LAGFLILSYYVVIAGWALHYIWKMADGTFAGATADVANQTFNDFLADPWALMFWQTVF
                     MAATVWIIARGVIKGLESAIRWFMPLLFVLLMVLLGYAVTSDGFSQGWDFMFDFNWSA
                     LDANAVLIAMGQAFFTLSLGMGAIMAYGAYLPSGTSITTTVATIAGLDTLVALAAGLA
                     IFPIVFANGLEPGQGPGLMFVTIPLAFGQMPLGLVFGTVFFVLVSFAAITSAISISEP
                     ALAYLVEQYNAKRSRVAITLGVIGWLLGVGTVLSFNHWADVHVVGELTYFEFV"
BASE COUNT          501 a         1030 c         1026 g          621 t
ORIGIN      
        1 cggcgggtcc tcgagctgtg caggctgcgc atcgcggcca ttcacgactg cgcccaggaa
       61 tgggggatcc gggatgccga ggtggccctg acgcagccgc agatggaagc gcttagaagc
      121 ggagacctct ccaacttcga cccccctgag cgggcggcat tggaactggc cgagcgcgtg
      181 ccttatcagc atcatcagct gactgatgaa gaggttgtgc atgcagaggg gctcctgggt
      241 gggccgggca cggtgtccct gctgacggcg ctcgctttct tcgacgtcgt ctgcaggctc
      301 aggctcacgc tggagctgga cgaggagagc gctttgctcg cgagcccgcc ccttgccgac
      361 ggcgccatgg tctgatcagg ggagcatccg atgtccacca agcctcgact ggcacccgca
      421 atccccggga gtgaagccga tttcgcatcg ctcatggctc aagcgcccaa ctccaccgcc
      481 ctttttttcg agctctacgc cgagttctgg cagaacggcg tcgtcgctga agagatcaag
      541 gagatgacgc gcatgcgcaa cgcgcgcatc accgactgcg gctactgaaa aaacgtacga
      601 ttcgactccg cccgtgcggc ggggctggac gaagcgaagg tcgagcagat cgcggacggg
      661 tacgagcgct cgctcgcgcc ggccgagacg gcggccctga agctgacgga cgccatcatc
      721 ggggcttctg gcccactcga tgcggatgcc aaggccgctc tcaaagctca cttctccgat
      781 gcgcagatcg tcgagatggc gctgggcgtc ggctgctttc tcggcatgtc caaggtgctc
      841 atcatgctcg ggctggagcc cgaggagatg ccggtgaccg tcgtgccgac accggggtcc
      901 tgaacgggcc cgcgtcggct cattcgggta ttcttcgccc tccgctgaca cagagcttgc
      961 cgacatggcg cagaccgcaa tagacatcag ccagctgccg atgatagaga acgccgggcg
     1021 ggtggcgatt ctcaagtcca agtggtatcc cgagctggtg gacagcatgg cgcgctgctg
     1081 cgagggggtc cttcgcgacc ggggcatcga ccgggtggag gttcacacgc tgccgggatc
     1141 cctggagctg cccctggccg ccgccgacct gcttgctgcc gactcgggcg gcgacatcga
     1201 cgcgatcgtc tgtttcggca tcatcctgaa gggcgaaacc ctgcacttcg agatgatcac
     1261 caacgagtgc atgcgcggcc tcggcgaggt catgcagcga taccggcggc cggtcatcgt
     1321 ggagatcctg cccgtactgg agatggccca tgcggaatca cgggccgccg atgacgagtt
     1381 caacaaaggg atcgaggcgg ctgcggccgc catcgagatg attgcctggc gccgggccgt
     1441 aggcgcttag cccgcctgct tcatgatccg ctgcttctgg cggttccagt cacgctcgcg
     1501 ctcggtggcc cgcttgtcat gcagcttttt gcccttgccc agcgctagct cgcacttgac
     1561 ccggttgccc ttccagtaca gcgctgtggc gatgcacgcg tagcccttct gccgggtggc
     1621 cgtgaagatc tgcgcgatct ctttcgtgtg gagcagcagc ttgcgggtgc gctgaggatc
     1681 cgcgaccaca tgggtcgatg cgctgggcag cggtgagatg ttggcgccca agagccacgc
     1741 ctcgccgtcg cgaacgagaa cgtagctgtc caccagctgc gccttgcccg cgcgcaggct
     1801 tttcacctcc cacccctcga gcaccaggcc ggcttcgaag gtctgctcga agtggtagtc
     1861 gcgcttcgcg cgcttgttca gcgcgatggt ttttggtgcg ggcttgcccg ttttgctgtt
     1921 caaatcgagg acctcatggc ctcggattat atgcggttga ggccccaaag acgttgggcc
     1981 acaatgccgg cccaacgcga tcgccttgga agtgctcatg gccccggcta ccgaatcccg
     2041 gcacggcatg tggtccagca gatggttgtt cgtgctggca gcagcgggat cggccgtggg
     2101 cctcgggaac atctggaagt tcccttacat agctggggag aacggcggcg gcgcgttcgt
     2161 catcgtctac ctcgtctgca tcgccctcgt cggcgtgccc atcatgatgg ccgaggtgat
     2221 gatgggcagg gaaggccgcc agagcccaat caacaccatg cgcacgctca ccgaagccca
     2281 tggccgctcg ccgctctggg tgctcatcgg ctggatgggc gtgctggccg ggtttctcat
     2341 tctcagctac tacgtggtca tcgccggatg ggccctgcac tacatctgga agatggccga
     2401 cggaacgttc gccggagcca ccgccgacgt tgccaaccag acattcaacg acttcctggc
     2461 cgatccctgg gcgctcatgt tctggcagac ggtcttcatg gcggccacgg tctggatcat
     2521 tgcccgcggc gtgatcaaag gtctcgagtc ggccatacgg tggttcatgc cgctgctgtt
     2581 cgtcctgctg atggtgctgc tcggttacgc ggtgacctcc gacgggttct cccagggctg
     2641 ggacttcatg ttcgacttca actggtccgc cctggacgcc aacgcggtgc tgatcgccat
     2701 gggccaggcc ttcttcacct tgagcctcgg gatgggcgcc atcatggcct acggggcgta
     2761 cctgccgagc ggcacgtcca tcaccaccac cgtggcgacc atcgcgggcc tggacaccct
     2821 ggtcgccctg gcggccgggc tggccatctt tcccatcgtt ttcgccaacg gcctggagcc
     2881 cggccagggc ccagggctca tgttcgtcac catcccgctg gcctttggcc agatgccgct
     2941 gggcctggtc ttcggtaccg tgttcttcgt tctggtgagc tttgccgcca tcacctccgc
     3001 catctccatc agcgagcccg cccttgccta cctcgtggag cagtacaacg ccaagcggtc
     3061 ccgcgtggcg atcacgctgg gcgtcatagg ctggctgctg ggcgtgggca cggtgctctc
     3121 cttcaaccat tgggcagacg tccatgtggt cggcgagctg acctacttcg agttcgtc
//
LOCUS       DBNHOX010000226         1951 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_211435, whole genome shotgun sequence.
ACCESSION   DBNHOX010000226 DBNHOX010000000
VERSION     DBNHOX010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_211435"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="AC16UZ_05020"
     CDS             complement(<1..233)
                     /locus_tag="AC16UZ_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6246077.1"
                     /translation="MATDAGHGGAAMEAQADEIGRLREEVARLGARLQHLEAVQAVRE
                     TLLDYAHYHDRGLSGELAELFAEDAVLDISGFG"
     gene            complement(237..>1951)
                     /locus_tag="AC16UZ_05025"
     CDS             complement(237..>1951)
                     /locus_tag="AC16UZ_05025"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6246078.1"
                     /translation="GGTVIDGTMIPRYRADVGIKDGRVARIGVGRKATAVRVIDAGDN
                     IVAPGYIDIHTHYDAQILWDPYCTISGWHGVTSVVLGNCGFGFAPVPPHLRERAMLMM
                     TRNEAIGFETMKEGMEWDRYGWETMPEWFEHLKRNVRLGVNATALVPLNPLYAYVMGG
                     IDEAKERRPTAAEKEEMIRLMYEALDHGACGFSYQRCGVPSVQPDWDGSPMITDMLPD
                     AEIIEFGRALGDYGRGFIEMFDAAPSDHDTVEAFMTSLARASGRPIVRNILLANDEYP
                     KAHRAFLEWLNEAHEEGLQVFGMGFTVRSPTVLTFEDWSLWDGFPGWNRVMNGPKEQR
                     AELMRNESMRVQLRKEMDTGRVGGLGTSGKPAKLIINDTAGYGELDHYLGRMVSEIAE
                     TENKHIVDVILDAVLASDFKVEFRGNAYDTSVETTMEVLNSPYVVPGISDGGAHTHFS
                     VQGAYPTDLLEWLVREERVMTAERAHYGLSRLPAHMCDLGDRGIIREGAPADLVIYNP
                     ETIRRTPSWDTLDKLRDLPANEWRLVQRAEGLNYTLVNGVVTFEGLECTGATPGELML
                     HGEV"
BASE COUNT          319 a          673 c          604 g          355 t
ORIGIN      
        1 tcgccaaatc cggagatgtc cagcacggca tcttcggcaa aaagctctgc cagctcgccg
       61 gaaaggcctc gatcgtggta gtgagcgtag tccagcaacg tctcccgcac cgcttgaacc
      121 gcttccaggt gctgcagccg cgcgcccagc cgggccacct cctcacgcag ccgaccgatt
      181 tcgtctgcct gcgcctccat cgccgctccc ccgtggccgg cgtccgtcgc caactctcag
      241 acctccccgt gcagcatcag ctcaccgggg gtcgcgcccg tgcactccag gccctcgaag
      301 gtgaccacac cgttcacgag ggtgtagttc aggccctccg cgcgctgcac caggcgccac
      361 tcgttggccg gcaggtcgcg cagcttgtcg agcgtgtccc aggagggcgt gcggcgaatg
      421 gtttccgggt tgtagatcac cagatccgca ggcgcgccct cgcgaatgat gccgcgatcg
      481 cccaaatcgc acatgtgcgc cggcaggcgg ctcagcccgt agtgggcacg ctcagcggtc
      541 atcacgcgct cctctcgcac gagccactcc agcagatcgg tgggatacgc cccctgcacg
      601 gagaagtggg tgtgggcgcc gccgtcggag atgccgggca cgacgtaggg gctgttgagc
      661 acctccatgg tggtctccac cgaggtgtcg taagcgttgc cgcgaaactc caccttgaag
      721 tcggatgcca gcaccgcgtc gaggatcacg tcgacgatgt gcttgttctc ggtctccgcg
      781 atctcggaca ccattcggcc cagatagtga tcgagctccc cgtacccggc cgtgtcgttg
      841 atgatcagct tcgccggttt gccgctggtc ccgagcccgc cgacgcggcc ggtgtccatc
      901 tccttgcgga gctgcacccg catgctctcg ttacgcatga gctcggcccg ctgctctttg
      961 gggccgttca tcaccctgtt ccagccaggg aagccgtccc agaggctcca gtcctcgaac
     1021 gtcagcacgg tgggcgagcg cacggtgaaa cccatgccga acacctgaag cccttcctcg
     1081 tgggcctcgt tcagccattc cagaaaggcc cggtgcgcct ttggatactc gtcgttggca
     1141 agcaggatgt tgcgaacgat gggacgaccg ctggcgcggg ccagggaggt catgaacgct
     1201 tcgacggtat cgtggtccga gggcgccgca tcgaacatct cgatgaaccc gcggccgtaa
     1261 tcgcccagcg ccctgccgaa ctcgatgatc tcggcgtccg gcagcatgtc cgtgatcatc
     1321 ggcgaaccgt cccagtcggg ctgaaccgag ggcaccccgc atcgctggta cgaaaagccg
     1381 cacgcaccgt gatcgagggc ttcgtacatg aggcggatca tctcctcctt ctctgccgcc
     1441 gtgggccggc gctccttggc ctcgtcgatt ccgcccatca cgtaggcgta aagcgggttg
     1501 aggggcacca gggcggtggc gttgacgccc aggcgcacat tgcgcttgag atgctcgaac
     1561 cactccggca tggtttccca gccgtacctg tcccactcca tcccctcttt catggtctcg
     1621 aagccgatgg cttcgttgcg agtcatcatc agcatggcgc gctcgcgcag atgcggcggg
     1681 accggcgcga agccgaaccc gcagtttccc agaaccaccg aggtgacccc gtgccagcca
     1741 gagatcgtgc agtacggatc ccacaagatc tgggcgtcgt agtgggtgtg gatgtcgatg
     1801 tatcccggag caacgatgtt gtcgccggcg tcgatcaccc ggactgcagt ggccttgcgg
     1861 ccgacgccga tcctggcaac cctgccgtcc ttgatgccga cgtccgcgcg atagcgcggg
     1921 atcatggtgc cgtctatgac ggtgccaccg c
//
LOCUS       DBNHOX010000227         8435 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_789772, whole genome shotgun sequence.
ACCESSION   DBNHOX010000227 DBNHOX010000000
VERSION     DBNHOX010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_789772"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /gene="argS"
                     /locus_tag="AC16UZ_05030"
     CDS             complement(<1..669)
                     /gene="argS"
                     /locus_tag="AC16UZ_05030"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase domain-containing protein"
                     /protein_id="HIR6246079.1"
                     /translation="MNVRALIGERIEAALAETGNPGAPALVQPASRPEFGDYQANGVM
                     GAAKRSGTKPRELADAVLKAAALDDVAAGVEVAGPGFLNITLADDFLSAQLLPEDRMS
                     AVEHPQTVVVDYSSPNLAKAMHVGHLRSTIIGDAVARVLEALGHTVIRQNHVGDWGTQ
                     FGMLLTHMTETGADSNTLSDLEDFYREAKARFDADPDFAERSRQTVVRLQSGDPDTRA
                     HWSRF"
     gene            complement(677..1183)
                     /locus_tag="AC16UZ_05035"
     CDS             complement(677..1183)
                     /locus_tag="AC16UZ_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR6246080.1"
                     /translation="MRTIVVAFATAIFFAAVIVELTDRLWPGSQVALLIVATIGLFLS
                     NLLALRLSGSPAAAAPAKRRSEAPGKQGQQKRPARQEKRPEPGTARSAAGPREPGKVK
                     WFNRNKGFGFIIRESGEEIFVHQRSIRPVGEGDNRRRPSLRDGQAVTFQVAERDKGIQ
                     AEDVVAED"
     gene            complement(1259..1834)
                     /locus_tag="AC16UZ_05040"
     CDS             complement(1259..1834)
                     /locus_tag="AC16UZ_05040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6246081.1"
                     /translation="MSNIARLLTTAILIGVAALAGPTAAQESGLPRVVVQTSVGSFEV
                     ELYPDRAPASVANFLSLVDEGFYDGLIFHRVIANFVVQTGGYDADMSYREPPRTVVNE
                     SANGLKNQRGYLAMARLNDPDSADAQFYINVRDNTHLDGKPGSPGYTVCGKVISGWEI
                     VVEIELSDPVVRAGMVGVPEVPIVIRRATRG"
     gene            complement(1856..2503)
                     /locus_tag="AC16UZ_05045"
     CDS             complement(1856..2503)
                     /locus_tag="AC16UZ_05045"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003626.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIR6246082.1"
                     /translation="MHPRPDQRHVRPDDVGDLADLLRAYPGIEYNIEGNPFAGPRDNG
                     YPGFDALIESARPHQATLVPDTDNQLTSDHGWDLTGDNGRLSDHINRYQAAGARVSLF
                     MDPVPEHIARARDVGADRIELYTGPFAQTVAEFGLDHEESLASLDRYQKAAACAAELG
                     LGVNAGHDLDTHNLRLFRQIEQVAEVSIGHALISDALEVGLTAAVRAYLDVLGHH"
     gene            complement(2571..3206)
                     /locus_tag="AC16UZ_05050"
     CDS             complement(2571..3206)
                     /locus_tag="AC16UZ_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HIR6246083.1"
                     /translation="MKSKSCLAALLMLAGLTLSARAETVYISDTFAVPLRSGPSNSHR
                     ILHRGLPSGTELTVLERDDEGQFARVRTTRGTEGWIPQQYLVAEPIARDRLDEASREI
                     ERLSSLVAEREAEIAQLRQAGARTWEEIEAGAQPGNRGPAGASAASPGERRLMELNER
                     LRHEFERLAVERDELKSSARQRWLLIGAGIMLAGIAIGVILKARPRRSGWS"
     gene            complement(3196..3756)
                     /locus_tag="AC16UZ_05055"
     CDS             complement(3196..3756)
                     /locus_tag="AC16UZ_05055"
                     /inference="COORDINATES: protein motif:HMM:NF016189.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane-spanning protein YciB"
                     /protein_id="HIR6246084.1"
                     /translation="MNQLLDFIPIVLFVAVFFLADIYYATAALMIAVTLQVLAYRLLK
                     KPIGRELALTFWASVIFGGLTLVFRNELFIQWKPTIVNWLLCASLLGSHFVGQRNLME
                     RMLGHQLTLVDQVWVRLNFGWAFGFFLAGALNLVVAYNFSMEFWVSYKLIGGFGLTLV
                     YIVITLVYLSKKGFLTDKPEGLGIEK"
     gene            3856..4467
                     /locus_tag="AC16UZ_05060"
     CDS             3856..4467
                     /locus_tag="AC16UZ_05060"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR6246085.1"
                     /translation="MSQYFVVHPSHPQRRLLVTAAQIISGGGLVVYPTDTAYALGCHI
                     GDKQSLESIRRIRRLDKRHHFTLTCRDLSEISLYARVSDANYRILKHLTPGPFTFLLP
                     ATREVPRRLVHPKRRTIGLRIPNNVIALELLAVLGEPMMTTTMRLADQDEPFSDPEQI
                     RESLEHEVDLIIDGGPGGVVPTTVVDLTGAVPEVIRQGLGVFE"
     gene            4531..5745
                     /locus_tag="AC16UZ_05065"
     CDS             4531..5745
                     /locus_tag="AC16UZ_05065"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR6246086.1"
                     /translation="MSSNESSHRVLSGMRPTGRLHLGQYHGVIRNWLELQHGYECFFF
                     VADWHALTTGYEDTGNIEQNTFDMVIDWLAAGVNPGTATVFVQSWVPEHAELHLLLSM
                     ITPVSWLERVPSYKDQQARLNDRDLSTYGFLGYPVLQSADILMYRAGNVPVGADQVAH
                     VELTREIARRFNHVYGREPGFEDLAIAAIGKMGKKAARLYKSLRRGYVEQGDGEALER
                     ARALLAEQSNLSIGDRERLFGYLEGGGRIILPEPQALLNKAAKVPGLDGEKMSKSYQN
                     TIAMREAPDEVEKKVRTMITDPARVRRDDPGDPANCPVFALHEIYSDAETRAWVTEGC
                     TTAGIGCIDCKKPLIDAINAEQEAMRERAQQFEDDRDLVHSILVEGSEKARDVARETL
                     DDVRAAIGLAHR"
     gene            5742..6578
                     /locus_tag="AC16UZ_05070"
     CDS             5742..6578
                     /locus_tag="AC16UZ_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR6246087.1"
                     /translation="MSIDTEGAAAPHLLKLREQGNDTLALVRGEAVTELPKDLYIPPQ
                     ALEVFLETFEGPLDLLLYLIRRENLDILDIRVAEITAQYMSYIELMTAMQLELAGEYL
                     VMAAMLAEIKSRMLLPRPAYADEDEDDPRAELVRRLQEYEQIKNAAEGIDALPRLERE
                     IFIASAERPEVVKRHAEPDVDLKEVLVSLAEVLRRAEMFQHHEVYLEPLSVRERMTAI
                     LDRVNSASDFVSFGELFRAEEGRRGVVVTFLALMELVRESLLDFVQNEPYAPIYVRAA
                     GE"
     gene            6583..7293
                     /gene="scpB"
                     /locus_tag="AC16UZ_05075"
     CDS             6583..7293
                     /gene="scpB"
                     /locus_tag="AC16UZ_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR6246088.1"
                     /translation="MNEAVDDARLKAVIDAVLLAAGEPVSVNQILALFGPGELEGEDV
                     RARVRACLRELESEAAGRGYQLKRVASGYRYQVRQELSEWVSRLWDEKPPRYSRALME
                     TLALVAYRQPLTRGDIEQVRGVSVSPNIMRTLLERGWVRVVGQREAPGRPSMYGTTRA
                     FLDYFNLKSLDQLPPLEEIRALIEPIVVNDEAEEELRPEITVEMPADMTELAPEREDA
                     ESPEPMTAEVVPLSSLPQ"
     gene            7336..8157
                     /locus_tag="AC16UZ_05080"
     CDS             7336..8157
                     /locus_tag="AC16UZ_05080"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR6246089.1"
                     /translation="MSPEKSEKLQKHLADLAYGSRREMEAWIRAGRVEVNGEAAHLGQ
                     RISSEDRICVDGRPVTGKRTDWARVLVYNKPAGEICTRRDPEGRSTVFDGLPRLQRGR
                     WIAIGRLDLQTSGLLLFTNDGALADRMMRPATGLDREYAVRVNGKLSESQERRLTDGV
                     MVDGERLQFSDIKYYDGRGTNHWYHVVLMEGRNREIRRLFESVGLSVSRLKRVRFGPV
                     ALPSTLPRGRYAELSYEDLKTLYGLLKLPLRARPAKGGRGPRTSFLLPYPDLPIV"
     gene            complement(8160..>8435)
                     /locus_tag="AC16UZ_05085"
     CDS             complement(8160..>8435)
                     /locus_tag="AC16UZ_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246090.1"
                     /translation="DDRVSGYLPWRETGNRVTARGDDLNKIWLTNQQQRDSQIMEDHN
                     VGAKIFEDVFEFEDDFETEAEANVEAFYVALKMALARLEAYATATPE"
BASE COUNT         1485 a         2639 c         2801 g         1510 t
ORIGIN      
        1 gaaccgggac cagtgggcgc gggtgtccgg gtctcccgac tgcagacgaa ccacggtctg
       61 gcggctgcgc tcggcgaagt cggggtcggc atcgaagcgt gctttggctt cccggtagaa
      121 gtcctccagg tccgaaaggg tgttcgagtc cgcgccggtt tcggtcatgt gtgtgagcag
      181 catgccgaac tgggtacccc agtcgcccac gtggttctgg cgaatgaccg tgtgcccgag
      241 ggcctcgaga acgcgcgcga cggcatcgcc tatgatcgtg ctgcgcagat ggccgacgtg
      301 catggccttg gcgagattgg ggctggagta gtccacgacc acggtttgcg ggtgctcgac
      361 ggccgacatg cgatcttccg gcagcagctg tgccgacagg aagtcgtctg ccagcgtgat
      421 gttcaggaag ccggggcccg ccacctccac gcccgccgca acgtcgtcca gcgctgcggc
      481 cttcaggacg gcgtcggcga gttccctcgg cttggtcccg gagcgctttg ccgctcccat
      541 caccccgttg gcctggtagt cgccgaactc gggacggctg gccggttgaa ccagagccgg
      601 ggcccccggg tttccggttt cggccagcgc cgcctcgatc cgttcgccga tcagtgcgcg
      661 gacgttcatg agcggatcag tcctcggcga cgacgtcttc tgcctgaata cccttgtcgc
      721 gctcggcaac ctggaaggtc accgcctgac cgtcgcgcag gctcggccgg cgtcggttgt
      781 ccccctcgcc cacaggtcgg attgatcgct ggtgaacgaa gatctcctcg ccggattcgc
      841 ggatgatgaa gccgaagccc ttgtttcggt tgaaccactt caccttgccc ggttcgcgcg
      901 gtcctgccgc gctgcgggcc gttccgggct ccggacgttt ttcctggcgg gccggtcgtt
      961 tctgctggcc ctgcttgccg ggcgcctcgg agcggcgctt tgcaggggcc gccgctgccg
     1021 ggctgccgga cagccgcagc gccagcaggt tactcaggaa caggccgatg gtggcaacga
     1081 tgagcagcgc cacctggctg ccaggccaca ggcgatcggt cagctcgacg atcacggcgg
     1141 caaagaagat ggcggtggcg aacgcgacga cgatggttct cataagcggt gtccaggcag
     1201 cgctgaagcg aaaaaacggt gaatcatacc caagcggatg tgcaattgct tgctggggtt
     1261 agcctcgcgt cgccctgcgg atgacgatgg gcacctcggg cacgccgacc atgccggcgc
     1321 gaaccaccgg atcggagagc tcgatttcta ccacgatctc ccagccggag atgaccttgc
     1381 cgcacaccgt gtagccgggc gaacctggct tcccatcgag gtgggtattg tcccgtacgt
     1441 tgatgtagaa ctgagcatcg gcggaatcag ggtcgttcaa gcgtgccatg gcgaggtagc
     1501 cgcgttgatt cttcagaccg ttggcggact cgttgaccac cgttcgcggc ggttcccggt
     1561 agctcatgtc tgcgtcgtag ccgccggtct ggacgacgaa gttcgcgatc acccggtgga
     1621 agatcagccc gtcgtagaaa ccctcgtcga cgagtgagag gaagttggct accgaggcgg
     1681 gagcgcggtc cggatagagc tctacctcga agctgcccac gctggtctga acgacgactc
     1741 tcggcagccc gctctcttgc gccgcggttg gaccggccaa ggcagcgacg ccgatcaata
     1801 tggcggtagt caggagtcgt gcgatgttgc tcatgatgcc caatcgtccg gcaggtcagt
     1861 ggtgtccgag gacgtccaga taagcgcgca cggctgccgt gagccccact tccagggcat
     1921 cgcttatcag cgcgtgtcct atggagacct ccgcgacctg ctcgatctgt cggaacagcc
     1981 gcaggttgtg ggtgtcgagg tcgtgcccgg cgttgacgcc gagcccgagc tcggcggcac
     2041 aggccgccgc tttctgatac ctgtccaggc tggcgaggct ttcctcatga tccagcccga
     2101 actctgccac ggtctgtgcg aacggaccgg tgtagagctc gatgcggtcc gccccgacgt
     2161 cgcgggcgcg ggcgatatgc tctggcaccg ggtccatgaa gagacttacc cgggcgccgg
     2221 cggcctggta gcgattgatg tggtcggata gtcggccgtt gtcgccggtg agatcccacc
     2281 cgtggtcgga ggtcagctgg ttgtcggtgt cgggcaccag ggtcgcctgg tggggccggg
     2341 cactttcgat gagcgcatca aaccccggat agccgttgtc tcggggaccg gcgaaggggt
     2401 tgccctcgat gttgtactcg ataccggggt atgcccgcag caggtcagcc aggtcgccca
     2461 cgtcgtcggg gcgcacgtga cgctggtccg ggcgtgggtg cacggtgctt gccgcgggcg
     2521 ttgcgcagca gggccacctt gttcaggttt acgctcagaa ccgccaagga tcagctccag
     2581 ccgctgcgac ggggacgggc tttgaggatc accccgatgg cgatgcctgc cagcatgatg
     2641 ccggctccga tcagcagcca gcgctggcgc gcgctgctct tcagctcgtc acgctcgacg
     2701 gccagccgtt cgaactcgtg gcgcagacgc tcgttcaact ccatcaggcg ccgctcgccg
     2761 gggctggcag cgcttgcgcc tgcagggccg cggttgccgg gctgggcgcc ggcctcgatc
     2821 tcctcccagg ttcgcgcccc tgcctggcgc agctgggcaa tctcggcttc ccgctccgcg
     2881 acgagactgc tcagccgctc gatctctcgg ctcgcctcgt cgagccgatc ccgcgcgatg
     2941 ggctcggcaa cgaggtattg ctgcgggatc cagccttccg ttcctcgggt ggtccgcacc
     3001 cgggcgaact ggccctcgtc gtcccgttcc agcacggtca gctccgttcc gctggggagc
     3061 ccccggtgga gtatccgatg cgagttcgac ggtccgcttc gaagcgggac cgcgaaggtg
     3121 tcggagatgt agaccgtctc ggcccgggcc gacagggtga ggccggcgag catgagcagg
     3181 gctgccaggc aggatttact tttcaatgcc aaggccctct ggcttatccg tgagaaaacc
     3241 tttcttgctc aggtatacca gcgtgatgac gatgtagacg agcgtgaggc cgaaaccccc
     3301 gatcagcttg tagctcaccc agaactccat ggagaagttg tacgccacca cgaggttgag
     3361 cgcgccggcg agaaagaagc cgaacgccca gccgaagttg agtctcaccc acacctgatc
     3421 caccaaggtc agctggtgac cgagcatgcg ctccatgagg ttgcgctgcc ccacgaagtg
     3481 gctccccagc agcgatgcgc acagaagcca gttgacgatg gtcggcttcc actgaatgaa
     3541 cagctcgttg cggaaaacca gggtgagccc gccgaatatg acgctcgccc agaaggtcag
     3601 cgccagctcg cggccaatgg gtttcttcaa aaggcgatag gcgagaacct gcagggtcac
     3661 ggcgatcatg agcgctgccg tggcgtagta gatgtcggca aggaagaaaa cggccacgaa
     3721 cagcacgatg gggatgaagt cgagcagctg attcatcgtt gattggccga atagccccgc
     3781 tgcgcgcggg gatcctggat aacgctaagc gtgtgataat acgagaaacc gagtgggcat
     3841 ggtaagcagg ggtctgtgag tcagtatttc gtcgtgcatc cttcgcaccc gcagcggcgg
     3901 cttctcgtca ccgcggctca gatcatctcg gggggcgggc tggtcgtcta tccgaccgat
     3961 acggcctacg cgctcggatg ccacatcggc gacaagcagt cgctggagag cattcgtcga
     4021 atccgccgcc tggacaagcg tcatcacttc accctgacct gccgggatct ctcggagatc
     4081 tcgctgtacg cccgcgtttc ggatgcgaac taccgcattc tcaagcacct caccccggga
     4141 ccgttcacgt tcctcctgcc ggcgacccgg gaggtcccac gtcggctggt tcacccgaag
     4201 cggcgcacga tcggcctgag aatcccgaac aacgtcatcg cgctcgaact gctcgccgtg
     4261 ctcggcgagc ccatgatgac gaccaccatg cggcttgccg atcaggacga gcccttttcg
     4321 gatccggagc agatccggga gagcctggag cacgaggtgg acctcatcat cgacggcggc
     4381 ccgggcggcg tggtgcccac cacagtcgtc gacctcaccg gagcggtgcc ggaggtgata
     4441 cggcagggct tgggcgtgtt cgagtgaacg ggtggttttg ccgggcccca gccctataat
     4501 gccgcctcag tttgtagttg agaggctgcg ttgagcagta acgagtcgag tcaccgggta
     4561 ctctcgggga tgcgtccgac cggccggctg cacctcggcc agtatcacgg cgtcatcagg
     4621 aactggctcg agctgcagca cggctacgaa tgtttcttct tcgtggcgga ctggcacgcg
     4681 ttgactaccg gctacgaaga caccggtaac atcgagcaga acaccttcga catggtgatc
     4741 gactggctcg ctgcgggtgt caacccgggt acggccaccg tcttcgtgca gtcctgggtg
     4801 cccgagcacg ccgagctgca tctgctgctg tcgatgatca ccccggtgag ctggctcgag
     4861 cgggtgccga gctacaagga ccagcaggcc cgcctgaacg accgcgacct ttcgacctac
     4921 ggctttctcg ggtaccccgt tctccagtct gcggacatac tgatgtacag agcgggcaac
     4981 gtgcccgtgg gtgccgatca ggtggcacac gtcgagctga cccgggagat cgcccgccgc
     5041 ttcaatcacg tctacgggcg ggagccgggg ttcgaagacc tcgcgatagc ggccattggc
     5101 aagatgggca agaaggccgc gcggctctac aaaagcctgc gacgaggata cgtcgagcag
     5161 ggggacggcg aggccctcga gcgagcgcga gccctgctgg cggagcagtc aaacctgtcc
     5221 attggcgatc gcgaacggct cttcggctac ctcgaaggtg gtgggcgtat catccttccc
     5281 gagcctcagg ccctgttgaa caaggcggcg aaggtgcccg ggctggacgg ggagaagatg
     5341 tccaagtcct accagaacac gatcgccatg agggaagctc ccgacgaggt ggaaaagaag
     5401 gtccgcacca tgattaccga tccggcgcgc gttcgccgtg acgatccggg cgatccggcg
     5461 aactgtcccg tattcgcgct gcacgagata tactcggacg ccgagacccg cgcctgggtg
     5521 accgaaggct gcaccaccgc ggggattggc tgcatcgact gcaagaagcc gctgatcgat
     5581 gccatcaacg cggagcagga agccatgcgc gaacgggcgc agcaattcga ggacgaccgc
     5641 gatctggtgc actccatcct cgtggaaggc tccgaaaagg cccgcgacgt cgcgcgggaa
     5701 accctcgacg acgtgcgcgc tgccatagga ctggcccacc gatgagcatc gacacggaag
     5761 gcgctgccgc tccccatctg ctgaagcttc gtgagcaggg caacgacacc ctggcgctcg
     5821 ttcggggcga agcggtaacc gagctgccca aggatctcta cattcctccg caggcgctcg
     5881 aggttttcct cgagacgttc gagggcccgc tcgatctgct tctgtacctg atccgcaggg
     5941 agaatctcga cattctggac atccgggtcg ccgagatcac cgcgcagtac atgagctaca
     6001 tcgagctgat gacggccatg cagctggagc tggccggaga gtacctggtg atggccgcca
     6061 tgctggcgga gatcaaatcg aggatgctgc ttccacgtcc ggcttatgcc gacgaagatg
     6121 aagacgaccc ccgtgcggag ctggtccgcc ggctgcagga gtacgagcag atcaagaacg
     6181 cggcagaggg catagatgcc ctgccgcgac tggaaagaga gatattcatc gccagtgccg
     6241 agcgcccgga agtcgtgaag cgccacgcgg agccggacgt cgacctcaag gaggtgctcg
     6301 tttcgctggc ggaggtgctg cggcgggccg agatgtttca gcatcacgag gtgtacctgg
     6361 agccgctatc ggtacgcgag cgcatgacgg ccatcctcga ccgggttaat tcggcaagcg
     6421 actttgtctc gttcggcgag ctgttcagag ccgaagaggg gcgcaggggt gtggtcgtca
     6481 ccttcctggc gctgatggag ctggtgcgcg aatccctgct ggatttcgtc cagaacgaac
     6541 cgtacgcgcc catttacgtg cgcgcggctg gagaatgagc ccatgaacga agctgtggac
     6601 gatgcgagac tcaaggccgt catcgatgcg gtcctgctcg cggcgggaga gccggtatcc
     6661 gtaaaccaga tcctcgcgct gtttggcccc ggcgagctcg aaggtgaaga cgtcagagcc
     6721 cgggtgcgcg cctgtctcag ggagctggag tccgaagccg ccggcagggg ctaccagctc
     6781 aagcgcgtgg ccagcggcta ccgctatcag gtccgtcagg agctcagcga gtgggtgagt
     6841 cgtctgtggg acgagaagcc gccgcgctat tcccgggcgc tgatggagac tctcgctctg
     6901 gtggcgtaca ggcagcccct gacccggggc gacatcgagc aggtccgcgg cgtcagcgtg
     6961 agccccaaca tcatgcgaac gctgctcgag cgtgggtggg ttcgcgttgt cggtcagcgg
     7021 gaagcgcccg ggaggccctc catgtacggg accacgagag cgtttctcga ctatttcaac
     7081 ctcaaaagcc tcgaccagct gccgccattg gaggagatca gggccctaat cgagcccatc
     7141 gtcgtcaacg acgaagccga agaggagctg cgcccagaga tcaccgtaga gatgccggcc
     7201 gacatgacgg agctcgcacc agagcgggaa gacgccgagt cgccagagcc catgaccgca
     7261 gaggtggtgc cgctttcgag cttgccgcaa taggggatcc ttccagccct taggggcgtt
     7321 ggcccccgga aaacgatgtc cccggaaaaa tcagagaaac tgcagaaaca ccttgccgac
     7381 ctggcttacg gttcgcggcg tgagatggaa gcgtggatcc gggccgggcg ggtcgaggtg
     7441 aacggcgagg cggcccatct cggccagcgg atctcgtctg aagaccgcat ctgcgtcgat
     7501 gggcggccgg tcacgggcaa gcgcaccgac tgggcgcgcg tgctcgtcta caacaagccc
     7561 gctggtgaga tctgcacccg ccgtgacccc gagggccgca gcaccgtctt cgacgggctg
     7621 ccccggctgc agcgcggccg ctggatcgcc atcggccggc tcgatctgca aacgagcggg
     7681 ctcctcctct tcaccaacga cggggccctt gccgatcgca tgatgcgccc ggcgacgggt
     7741 ctcgaccggg aatacgcggt ccgcgtcaac ggcaagctca gcgaaagcca ggagcgtcgg
     7801 ctcaccgatg gggtgatggt cgacggcgag cggctgcaat tctcggacat caagtactac
     7861 gacgggcgtg gcaccaatca ctggtatcac gtggttctga tggagggccg gaatcgtgag
     7921 atcaggcgac tgttcgagag tgtcggcctg tcggtcagcc ggctcaagcg ggtccggttc
     7981 ggtcccgtgg cgcttccttc taccctccct cgcggacgct atgccgagtt gagttacgag
     8041 gatctgaaaa ccctgtacgg cctgctcaag ctgccgctgc gggctcgccc cgccaagggc
     8101 gggcgtggtc cgcgcacctc ttttctgctg ccgtaccccg acctgccgat cgtctgaggc
     8161 tattccggcg tggcggtcgc gtacgcttcg agccgggcga gtgccatctt gagggcgacg
     8221 tagaacgctt ccacgtttgc ctctgcttcg gtttcgaaat cgtcctcaaa ctcaaagacg
     8281 tcttcgaaga tctttgcgcc gacgttgtga tcttccatga tctgactatc ccgttgttgt
     8341 tggttggtga gccagatctt attcaagtcg tccccccggg ctgtgacgcg gttcccagtt
     8401 tcgcgccatg gcaggtaccc gctgacccgg tcatc
//
LOCUS       DBNHOX010000228         1859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_853767, whole genome shotgun sequence.
ACCESSION   DBNHOX010000228 DBNHOX010000000
VERSION     DBNHOX010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_853767"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="AC16UZ_05090"
     CDS             complement(<1..242)
                     /locus_tag="AC16UZ_05090"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6246091.1"
                     /translation="MELIVFDLDGTLLNKSAEISDYTRDTLSMLADRGILYTVATGRT
                     LHASVEVLEGHGFRLPQAYKNGVMIWNPTLGDYTHS"
     gene            384..1283
                     /locus_tag="AC16UZ_05095"
     CDS             384..1283
                     /locus_tag="AC16UZ_05095"
                     /inference="COORDINATES: protein motif:HMM:NF006128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR6246092.1"
                     /translation="MSQKSYENIKYEVDNGRARISLNRPEKRNALSIELVEELRDALW
                     EADDDTAVHCVILKGEGPSFCAGYDLTAGRSKVDDGVKRRSGASFDDDAWRIERFQRS
                     LRTLAEMHKPSIAQIHGNCLAGGTDLALYCDILICADNANIGYSALRNMGAAPNQMWL
                     YHCGPQWTKRLLLTGDTLSGEDAARIGLVLKSVPEAYLESEVEGLADRLSWIDPEMLS
                     TNKRIINVGMELMGAQVLQRLAAENDARAHTTQAARNVFKQIATEGLRSALRDRDAPF
                     GDSRARVIGPETRDEQGRLVPWE"
     gene            complement(1314..>1859)
                     /locus_tag="AC16UZ_05100"
     CDS             complement(1314..>1859)
                     /locus_tag="AC16UZ_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012922.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIR6246093.1"
                     /translation="SGVVNAFIEITPFDTVKYEVDKESGYLRVDRPSRTSSLPPTLYG
                     FVPRTYCGDRVKVLMEGADSGDFDPLDICVISDRLVNRAEVIIPARVVGGLPMLDGGE
                     ADDKIIAVHANDEIWRDTNDIGDLPEQYVARLRHYFASYKTLPGESSTVHVGEPYGRE
                     RALRVLHASISDYQEQFPANP"
BASE COUNT          361 a          552 c          623 g          323 t
ORIGIN      
        1 ttgctgtggg tgtagtcccc aagcgtcggg ttccagatca tcacaccgtt cttgtacgcc
       61 tggggcaggc ggaagccgtg gccctcgagg acctcgacgg acgcgtgcag cgttcggccg
      121 gtggcgaccg tgtacaggat gccgcggtcc gccagcatcg aaagcgtgtc gcgggtgtaa
      181 tcggagatct ccgcggattt attcagcagg gtgccgtcga ggtcgaaaac gatcagttcc
      241 atccggcgca gtgttgcaga ctgagcggct gggtcaaacc tttcgggatt cgccgtgctc
      301 cgggaggttc caacggttgc tgctacccgt ggggcgcagg caatatccgg aactgccgct
      361 accgcgcatc cacaggagat cccatgagcc agaagtcata cgagaacatc aagtacgagg
      421 tcgacaacgg acgagcccgg atctctctga atcggccgga gaagcgcaac gcgctgtcca
      481 tcgagctggt cgaggagctc agagacgcgt tgtgggaagc cgacgacgat acggccgttc
      541 actgcgtcat tctgaaaggg gagggcccct cgttctgcgc gggctacgac ctgacagccg
      601 gtcggtcgaa ggtggatgac ggggtcaagc gccgttctgg cgccagcttc gacgacgatg
      661 cgtggaggat agaacggttt cagcgcagtc tgcgaacgct tgccgagatg cacaaaccgt
      721 ctattgccca gatacacgga aactgtctgg caggcggcac ggacctcgcg ctgtactgcg
      781 acattctgat ctgcgccgac aacgcgaaca ttggttactc ggccctgcgc aacatgggcg
      841 cggcgccgaa ccagatgtgg ctgtatcact gtggcccgca atggaccaag cgtcttctgc
      901 tgacagggga cacgctgagc ggcgaggatg cggccagaat agggctcgtg ctgaagtccg
      961 tgcccgaagc gtacctggag agcgaagtgg aagggcttgc ggaccggttg agctggatcg
     1021 atccggaaat gctctccacc aacaagcgga tcatcaatgt gggcatggag ctcatgggtg
     1081 cccaggtgct gcagcggctg gcggcggaga acgatgcccg ggctcacacc acgcaggcgg
     1141 cgcgaaacgt gttcaagcag atagccaccg aagggctgcg ctccgcgctt cgggaccggg
     1201 atgcgccgtt cggcgacagc cgtgccaggg tcatcgggcc ggaaaccagg gacgagcagg
     1261 gaaggctggt gccctgggag tgatcagagg gcccgcagcc gcacccggtg ggctcagggg
     1321 tttgcgggaa actgttcctg gtagtcggaa atcgacgcat gcagaacccg cagcgcccgc
     1381 tcgcgcccgt acggctcgcc cacgtggacg gtcgacgatt ccccgggaag ggtcttgtac
     1441 gaggcgaagt agtgccgcag gcgggcaacg tattgctcgg gcaggtcgcc aatgtcgttg
     1501 gtgtcgcgcc agatctcgtc gttcgcatga accgcgatga tcttgtcgtc ggcctccccg
     1561 ccatcgagca tcggtagccc gccaaccacc cgtgccggga tgatgacctc ggcgcggttg
     1621 accagccggt cactgatcac gcagatgtcc agagggtcga aatctcccga gtccgcccct
     1681 tccatcagca ctttcacacg gtctccgcaa tacgtgcgcg gtacgaaacc gtacagcgtc
     1741 ggtggcagcg aggaggtgcg gctcggccgg tccacccgca gataacccga ctccttgtcg
     1801 acctcgtatt tcaccgtgtc gaaaggcgtg atctcgatga aggcgttgac gacaccgga
//
LOCUS       DBNHOX010000229         2549 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1561525, whole genome shotgun sequence.
ACCESSION   DBNHOX010000229 DBNHOX010000000
VERSION     DBNHOX010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1561525"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..558
                     /locus_tag="AC16UZ_05105"
     CDS             88..558
                     /locus_tag="AC16UZ_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008367625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR6246094.1"
                     /translation="MPTYDRADRLILETLQNDASLSNAELAERIGLSANACWRRTRRL
                     EERGVIRKQVALLSQEKLNLKVTVFVGIRTNEHNESWLERFAEGVKAIPEVVEFYRMS
                     GDTDYLLKIVAEDIADYDRVYKHLISVANLHDVSSSFAMERIKSSTALPLTHLP"
     gene            complement(591..1208)
                     /locus_tag="AC16UZ_05110"
     CDS             complement(591..1208)
                     /locus_tag="AC16UZ_05110"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HIR6246095.1"
                     /translation="MADLAVVANVVLWACALALAVLVLALTRQVRSLHGRIAPAGALS
                     VNQALKVGMRAPSFNLETLGGGEVAIAAPGARQLLFFLAPDCPISRSMLPVLRSVHRA
                     EGGVALVLASDGDDPGEHLSFVAEMQLGEFPYVVSEELGRAYGVSKVPYAVLIDSDAR
                     IGALGIVNSREHLESLFEASERGVGSIQDYLAREGGGQPRYFEVR"
     gene            complement(1201..1722)
                     /locus_tag="AC16UZ_05115"
     CDS             complement(1201..1722)
                     /locus_tag="AC16UZ_05115"
                     /inference="COORDINATES: protein motif:HMM:NF018942.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HIR6246096.1"
                     /translation="MWEWVVHKVAAGTIACVFALALYHKGRDFPRFRASFEAYGIVPS
                     FLVPVCAPLLMGLEAGALVCLFLPDGPGSLLAFSVLGIYSVAIAVNLIRGRDYIDCGC
                     GDLPTPLSGWLILRNGLLMALAWPYGAAPEVAAVEPVTWLLVVMTVVVLMSLYLILEQ
                     LLANAGVGVANRG"
BASE COUNT          527 a          822 c          754 g          446 t
ORIGIN      
        1 tggagaggta aaaagtcccg ttatgtttca catattcctg gttatacgta atattattct
       61 acgattctta ctaatttcag acgaattatg cctacttacg atcgcgctga ccgacttatt
      121 ctggagaccc tgcagaacga cgcttctctt tccaatgccg agctcgccga gcgcatcggg
      181 ctgtccgcga acgcctgctg gcgccgcacc cgccgcctgg aggaacgggg ggtcattcgc
      241 aagcaggtcg cgctcttgag ccaggagaag ctgaacctca aggttaccgt tttcgtgggc
      301 atccgaacga acgagcacaa cgagagctgg ctggagcgat tcgcagaagg ggtcaaggcc
      361 atcccggagg tcgtggagtt ttaccgcatg agcggggata ccgactacct gctgaagatc
      421 gtggccgagg acatcgccga ttacgacagg gtctacaagc atctgatttc agtcgcaaac
      481 ctgcacgacg tgtcatcctc cttcgccatg gagcggatca agtcctccac ggccctgccg
      541 ctgacgcacc ttccatgagg atgtcctgac ctagcggggc ggcacgagca ttaacgaacc
      601 tcgaaatagc gcggctggcc tcccccttcc cgggccagat agtcctgaat ggacccgacc
      661 ccgcgctcgc tggcctcaaa caggctctca agatgctccc tggagttgac gatccccagc
      721 gcgccgatcc gagcgtcgga atcaatcagc acggcatagg gcaccttgct tacaccatag
      781 gctcgaccga gctcctcgga gaccacgtag ggaaactcgc cgagctgcat ttcggcgaca
      841 aaagacaggt gttcccccgg gtcatctcca tcgctggcaa gcaccagggc gacgccgcct
      901 tcggcacggt gcaccgaccg gagcacgggc agcatcgacc ggctgatggg acagtcaggg
      961 gcaaggaaga agagcagttg acgcgccccg ggcgcggcga tcgccacctc tcccccaccg
     1021 agggtttcca ggttgaacga cggggcccgc ataccaacct tgagcgcctg attcacgctg
     1081 agcgccccgg ccggcgcgat tcgcccgtgt agcgaacgca cctgccgagt cagcgcgagc
     1141 acgaggacgg caagggcaag agcgcaagcc cagagcacga cgttcgcgac aacggcaagg
     1201 tcagccacgg ttcgcgacgc ccacgcccgc gttggcgagc agctgctcca ggatcagata
     1261 caggctcatc agcacgacca cggtcatcac gaccagcagc caggtgacgg gctcgacagc
     1321 cgcgacttcc ggtgccgctc cgtaaggcca cgcaagggcc atcagcaggc cgttgcgaag
     1381 aatcagccac ccgctcaggg gagtggggag atcaccgcat ccgcaatcga tgtaatccct
     1441 gccgcgtatg aggttcacgg ctatagccac gctgtagatt ccgagcaccg agaatgcaag
     1501 cagcgagccg ggaccgtccg gcagaaagag gcagacgagg gcgccagcct ctagccccat
     1561 tagaagcggg gcgcagaccg gaacaaggaa actcgggaca attccgtagg cctcaaaaga
     1621 tgctcgaaac cgcggaaagt cccgcccctt gtgatacagc gcgagcgcaa acacgcaggc
     1681 tatggtgccc gcagccacct tgtgaacgac ccattcccac attgcagtcg cgcccggctt
     1741 cccctctgac tcaccgctct ctccggaccg taaagccgtc cacgtccgga aacccttcgc
     1801 cacggaggtt agcattgcat gtgggcgcgc gtacaatggc gcgctttcca gctgaggcgg
     1861 cattgtgagc gggcagagcc cacccctgtt cagtgaagcg acacgtcgct acgttctcgg
     1921 catcccaatt gcccgctggg catacctttc ggcatcctct ccggttttct ggcgggcggc
     1981 tggatgaagg agttcttcgg ctgacgggtc gcattcttcg tcgtcgggat accccgcatc
     2041 gtagcggctg tggttgttcg gctgaccgtc cgcgaaccgc ccagcggtat ggccgaggga
     2101 cgcgaaccgg ccatcgagca gcccaccatc atggcgacgt gccgaacgct gtggcgaaga
     2161 agggcattcc gggcggtatc ggaatcgccc tcggaggcca tctggcggac cggctcggcg
     2221 cccgcgatcg gtggcataag aaatactgat ttttgttctt aatatcatta ggttaggcct
     2281 gtaacctaaa accgtaggca ttatcagcga tctcctccaa cctgcctacg ggccggagtc
     2341 gctccgctac agccttctcg tctgctcgtt cgtgaatgtc tgggctgccg cccactatta
     2401 cccggcggga acatacctcc caggcgacct ggcggccgca gagcgcggca gcgactgacc
     2461 accgagccta tggtttcggt tcatcggcat gccacagatc gcgggtcccg atccgaagga
     2521 attccgccgg cagccgcgag gtaccctcg
//
LOCUS       DBNHOX010000230         1569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1432728, whole genome shotgun sequence.
ACCESSION   DBNHOX010000230 DBNHOX010000000
VERSION     DBNHOX010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1432728"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="AC16UZ_05120"
     CDS             <1..690
                     /locus_tag="AC16UZ_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6246097.1"
                     /translation="GSLGGMQAMRWATDYPDRLHAAVLIACAARLSAQNIAFNEIARQ
                     AIISDPEFHGGNYRQLGVNPDLGLMLARMVGHVTYLSDDGMRQRFGRQLKSGDIETAQ
                     DVEFQVESYLHHQGRSFSQRFDANSYVLMTRALDYFDPAREHDDDLVKALASATCRFC
                     VISFTTDWRFSVARSKELVDALIAAGKNVASAIIESEHGHDSFLLNVPRYFDVLRAYL
                     GRIATEAVDAP"
     gene            680..1288
                     /gene="metW"
                     /locus_tag="AC16UZ_05125"
     CDS             680..1288
                     /gene="metW"
                     /locus_tag="AC16UZ_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008364885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HIR6246098.1"
                     /translation="MRPDLEIIAEVIKPGARVLDLGCGEGELLAYLQTSKQVNGYGLE
                     IDPDNITTCLHNGVNVIEQDLDEGLDNFPDDSFDLVVMTETLQAVQNPDRMLDDMLRI
                     GGECIVTFPNFGYWRCRAYLALRGRMPVSEHLPHAWYDTPNIHLCTFKDFERLCHSKG
                     LPVIDRFVVDERHLARRLMQRFPNFFGHIAFYRLGRPNSGEA"
     gene            1288..>1569
                     /locus_tag="AC16UZ_05130"
     CDS             1288..>1569
                     /locus_tag="AC16UZ_05130"
                     /inference="COORDINATES: protein motif:HMM:NF025822.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4426 domain-containing protein"
                     /protein_id="HIR6246099.1"
                     /translation="MVKTGAGWICALILSWAGTPGHAEQRQSFGGWDVHYVVLPTGFL
                     KPAIAAQYGITRGRDRALVNISVLDPTGAPQKVSIEGTVKNLLGQLQPLA"
BASE COUNT          279 a          516 c          487 g          287 t
ORIGIN      
        1 ggcagcctgg ggggcatgca ggccatgcgc tgggcaaccg actatcccga tcgccttcac
       61 gccgccgtgc tgatcgcctg cgccgccagg ctgtccgctc agaacatcgc tttcaacgag
      121 atcgcacggc aggccatcat ctcggatccg gagtttcacg gcggcaacta ccggcagctg
      181 ggcgtcaacc cggacctggg cctcatgctc gcgcgcatgg taggtcacgt cacctatctg
      241 tccgacgatg gcatgcgcca gcgcttcggc cggcagctga agtcagggga tatcgaaacc
      301 gcccaggacg tggagtttca ggtggagagc tatctgcacc accagggccg ctcgttctct
      361 cagcgcttcg acgccaacag ctacgtgctg atgaccaggg cactggacta cttcgacccg
      421 gcccgggagc acgacgacga cctggtcaaa gcgctggcat ccgccacctg ccgcttctgc
      481 gtcatctcgt tcaccacgga ctggcgcttc tccgtggcgc gctcgaagga gctggtggac
      541 gcgctcatcg cggcgggcaa gaacgtggcc agcgccatca tcgagtcgga gcacggccac
      601 gactcgtttc tcctgaacgt gccgcgatac ttcgacgtgc ttcgggccta tctgggccgc
      661 atcgccacgg aggccgtcga tgcgccctga cctggagatc atcgcggagg tgatcaaacc
      721 tggagcccgg gtgctcgatc tcggttgcgg cgagggcgag ctgctcgctt atcttcagac
      781 ttcaaagcag gtcaacggct acgggctgga aatcgatccc gacaacatca ccacctgcct
      841 gcacaacggc gtcaacgtca tcgagcagga tctcgatgag gggctcgaca attttccgga
      901 tgacagcttc gacctggtgg tgatgaccga gacgctgcag gcggtgcaga accccgaccg
      961 catgctcgac gacatgctcc ggatcggcgg cgagtgcatc gtcacgtttc cgaatttcgg
     1021 ctactggcgt tgccgcgcgt atctggcgct gcgcggccgt atgcccgtct ccgagcacct
     1081 gcctcacgcc tggtacgaca cgcccaacat ccacctgtgc accttcaagg atttcgaacg
     1141 gctgtgccac agcaaaggcc taccggtcat cgatcgcttc gtcgtcgacg agcggcatct
     1201 cgcaagacgg ctgatgcagc gatttcccaa cttcttcggt cacatcgcct tctataggct
     1261 gggcagaccg aattcgggag aggcctgatg gtgaagacgg gcgctggctg gatctgcgcg
     1321 ctcatcctgt cgtgggcggg gacgccggga cacgccgagc agcggcagtc cttcggtggc
     1381 tgggacgtgc actacgtggt gctgccgacc ggcttcctga aaccggccat cgccgcgcaa
     1441 tacgggatca ctcggggccg cgaccgcgcc ctggtcaata tttccgtgct cgacccgacg
     1501 ggcgctcccc agaaggtctc catcgaaggc accgtgaaga atctgttggg tcagctgcag
     1561 ccgctggcg
//
LOCUS       DBNHOX010000231         1550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1176111, whole genome shotgun sequence.
ACCESSION   DBNHOX010000231 DBNHOX010000000
VERSION     DBNHOX010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1176111"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..825)
                     /gene="pgeF"
                     /locus_tag="AC16UZ_05135"
     CDS             complement(73..825)
                     /gene="pgeF"
                     /locus_tag="AC16UZ_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014619.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HIR6246100.1"
                     /translation="MVEANAWEWARWPDDAPGHRIAAVTTTRPGGFSKGNCAAFNLAC
                     HVHDDPAHVARNRRDLLKALGLAHVQWLEQVHGTGCVQATLASCDRVPVADAAWTSVP
                     GLALAVLTADCLPVVVAARDASTVAVIHAGWRGLVAGIIPSVVGSLPGRTTGYVGWIG
                     PGIGADAYEVGGDVAEAVRQSGPEAEAYLRPGGRADKYQLDLAGLAVRQLGDVGIGFV
                     ERSSACTWSDARFYSYRRDGETGRQATLVWIR"
     gene            complement(818..>1550)
                     /locus_tag="AC16UZ_05140"
     CDS             complement(818..>1550)
                     /locus_tag="AC16UZ_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR6246101.1"
                     /translation="TGEPVPFDVLYEDADLLVVNKPAGLVVHPGAGNPDGTLVNGLLH
                     HRASLALVPRAGIVHRLDKDTSGLLLVAANLEAHAALVRALQARQIQRGYRAVCEGVL
                     VSGLDIDAPMGRDPVRRTLQRVRADGRPALTRVRVVERFRAHTLVVAQLETGRTHQIR
                     VHLSSIGHPLVGDRAYGARGRLPPGAQDELIAALRGFSRQALHAAKLAFDHPVHGGRL
                     QFEAPDPDDLVDLLDALRQDVRHHG"
BASE COUNT          273 a          555 c          493 g          229 t
ORIGIN      
        1 ccagcagggc gttgcagaga actgcttgtc ctgaggaccc ggggcccgca gccgtgcggg
       61 cccactgcca gctcaccgta tccagaccag cgttgcctgg cgaccggtct cgccatcgcg
      121 ccgatacgaa taaaacctgg cgtcggacca ggtgcatgcc gaggatcgct cgacaaatcc
      181 gatccccacg tccccaagct gccgaacggc cagcccggcc aggtcaagct gatatttgtc
      241 cgcccgtccg cccggtctca ggtaggcttc cgcctcgggg cccgattgcc gcaccgcctc
      301 ggccacgtct ccgccaacct cataagcgtc ggctccgatt cccggaccaa tccagcccac
      361 gtatcccgtc gtcctgccag gtagcgagcc caccacgctg gggatgatgc cggcaaccag
      421 gccgcgccaa cccgcgtgga tcacggcaac cgtgctcgca tcgcgagcag caacgaccac
      481 cggcaggcaa tcggcggtaa ggacggccag cgcaagcccg ggaacagacg tccacgcggc
      541 atcggccacc ggcacccggt cgcagcttgc cagggtggcc tggacacagc ctgttccatg
      601 gacctgctcg agccattgca cgtgcgcaag accgagcgct ttgagcagat cgcgacggtt
      661 tcgggccaca tgggccgggt catcgtggac gtggcaggcg agattgaagg ctgcacaatt
      721 ccccttgctg aacccgcccg gccgggtcgt ggttaccgcg gcgattcggt ggccgggcgc
      781 gtcgtccggc cacctggccc actcccaagc gttcgcttca accatggtgc cgcacgtcct
      841 gccgcagggc atcgagcaaa tccaccagat cgtcggggtc cggagcttcg aactgcaacc
      901 tcccgccgtg aacggggtgg tcgaaggcga gcttcgcggc atgcagcgcc tggcgcgaga
      961 aaccgcggag cgcggcgatc agttcgtcct gagcacccgg tggcagacgt ccccgagccc
     1021 cgtaggctcg gtcccccacc agcggatgcc cgatactgct cagatgcact ctgatctggt
     1081 gggtgcgtcc ggtttccagc tgcgcgacca ccagggtgtg agccctgaaa cgctcgacca
     1141 cccggacccg ggtgagcgcg gggcgaccat cggcccgaac gcgctggagc gtcctgcgca
     1201 cgggatcgcg ccccatcggg gcatcgatat ccagcccgct gaccagcacc ccttcacaga
     1261 cggcgcgata gccccgctga atctgtctgg cctgcagcgc cctgacaaga gcggcgtgag
     1321 cttcgagatt ggccgcaacc aggagcagcc cggacgtgtc cttgtccaga cggtgcacga
     1381 tccccgcccg cggaaccagg gccagcgagg cccggtgatg gagcagcccg ttgacgagcg
     1441 tgccatcggg attgcccgct ccaggatgaa ccactagccc cgcaggcttg ttgacgacga
     1501 gcaggtcggc gtcttcatag aggacgtcaa acgggacggg ctccccagtg
//
LOCUS       DBNHOX010000232         1781 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1626123, whole genome shotgun sequence.
ACCESSION   DBNHOX010000232 DBNHOX010000000
VERSION     DBNHOX010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1626123"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="AC16UZ_05145"
     CDS             <1..1014
                     /locus_tag="AC16UZ_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6246102.1"
                     /translation="FHKYSVWDTGETILRTALMPRVREAYGFAYYHAWRPDLIAALTN
                     NLSAGVNMNRKVVRVGQDDGKAWAECEDGERVEGDVLIGADGIHSLVREQVFRPDSPR
                     ASGYVTWRGVVESETVADLDIPVSAYVDMGPRLSFVYYYVASGRLLNWLALGQANDDK
                     RESWSQTASKSEVLAAFDGWYERPRRLIEATEQPFVTALHDRNPLESWVSGRIAVMGD
                     AAHAMLPYHAQGAVQSIEDAWVLARTLELHGDDPHGALTRFESLRYQRANLLVQQSRN
                     AERWYHLDDPVEVAARNERFRRNNDRYGDDFSPQQHWLYSYDAEAAARGTDEEWRALP
                     TWS"
     gene            complement(1024..1581)
                     /locus_tag="AC16UZ_05150"
     CDS             complement(1024..1581)
                     /locus_tag="AC16UZ_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013053.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR6246103.1"
                     /translation="MQRLDPKSTAVIVIDVQERLAPAMPEAHLAELLRASRILLRGAA
                     LFGSPVLATEQYPRGLGATVPELGEQLGELNVAPREKLSFSACDDEAFMAELEASGAR
                     AAVVVGMESHVCVYQTVRDLVGAGLDVHVPIDGVCSRRDDHRQAGIDLCVRAGAVRTT
                     TESVAFDWLRVAGSPEFKELSGLIR"
     gene            complement(1602..>1781)
                     /locus_tag="AC16UZ_05155"
     CDS             complement(1602..>1781)
                     /locus_tag="AC16UZ_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246104.1"
                     /translation="EKMVADMYQRGKAVNMASHFELDEVIDPAESRRWIVAGLRSVPP
                     APRRSGKKRPNVDTW"
BASE COUNT          292 a          615 c          587 g          287 t
ORIGIN      
        1 ttccacaagt acagcgtctg ggacaccggc gagaccattc tgcggacggc gctgatgccc
       61 cgggtacggg aagcgtacgg ctttgcctac tatcacgcct ggcgcccgga cctcatcgcc
      121 gcgctgacca acaacctttc ggccggggtg aacatgaacc ggaaggtcgt ccgcgtgggt
      181 caggacgacg gcaaggcctg ggcggagtgc gaggacggcg agcgcgtcga gggcgacgtg
      241 ctcatcggcg cagacggcat tcactccctc gtccgggagc aggtcttccg gccggactcg
      301 ccgagagcgt ccggatacgt gacctggcgc ggggtcgtcg agtccgagac ggtggccgat
      361 ctggacattc ccgtgtccgc ctacgtggac atgggaccgc ggctgtcctt cgtctactac
      421 tacgtcgcct ctggccggct gctgaactgg ctcgccctgg gacaggccaa cgacgacaag
      481 cgcgagtcct ggtctcagac cgccagcaaa tccgaggtgc tggccgcttt cgacggctgg
      541 tacgagcggc ccaggcgcct gatagaggct accgagcagc ctttcgttac cgcgctgcac
      601 gaccgcaacc cgctggagag ctgggtgtcc gggcgcatcg ccgtcatggg cgatgccgcc
      661 cacgccatgc tcccctacca tgcccagggc gcggtgcagt ccatcgagga tgcctgggtg
      721 ctggcccgaa ccctcgagct tcacggcgac gacccccacg gggcgcttac gcggttcgaa
      781 tcgctgcgct accagcgcgc caacctgctg gtgcagcagt cgcgcaacgc cgagcgctgg
      841 tatcacctgg acgatcccgt cgaggtggcc gcacgcaacg agcgtttccg ccggaacaac
      901 gatcgctacg gtgacgactt ctcaccgcaa cagcactggt tgtacagcta cgatgccgag
      961 gccgctgcgc gcggaaccga cgaggagtgg cgcgcgctgc cgacttggtc ctgaccgggc
     1021 cgcctaccga atcagcccgg acagctcctt gaactctggg cttcccgcca cgcgcagcca
     1081 gtcgaaggcg accgactcgg tggtcgttct aacggctccc gcgcgcacgc acagatcgat
     1141 ccccgcctgc cgatgatcgt cgcggcgcga gcacacgccg tctatgggca cgtgaacgtc
     1201 gagaccggcg ccaacaaggt ctcgaacggt ctggtagacg cagacgtgcg attccatgcc
     1261 gaccaccacc gcggcgcgag cgccgctggc ttcgagctcc gccatgaaag cctcgtcgtc
     1321 gcaggcgctg aagctgagct tctcgcgcgg cgccacgttg agctcgccga gctgctcgcc
     1381 aagctccggg acggtggccc ccagcccgcg aggatactgc tcggtggcca gcacgggaga
     1441 gccgaacagc gccgcgccgc gaagcaggat gcggctggcg cgcaggagtt ccgccaggtg
     1501 cgcctccggc atggcgggcg cgaggcgctc ctgaacgtcg atgacgatga cggcagtgga
     1561 tttcgggtcc agacgctgca tatgctctcc ccttgcttgg gctaccaggt gtctacgttc
     1621 ggccgtttct tcccggaccg ccgcggtgcc ggaggcaccg agcgaagtcc tgcgacgatc
     1681 cacctgcgag attccgccgg gtcgatgacc tcgtcgagct cgaagtgcga ggccatgttc
     1741 accgccttgc cgcgctggta catgtcggcc accattttct c
//
LOCUS       DBNHOX010000233         5176 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_276449, whole genome shotgun sequence.
ACCESSION   DBNHOX010000233 DBNHOX010000000
VERSION     DBNHOX010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_276449"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="AC16UZ_05160"
     CDS             complement(<1..1419)
                     /locus_tag="AC16UZ_05160"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6246105.1"
                     /translation="MLVALFSSGVTLAADHASRIEAHVESSATPWTGLQANDAEEDFT
                     FVVVADRTRGHRPGVFESAMPKVNLLQPAFVVSVGDLIEGYTDDPARLTGEWDEVEAF
                     VDQLDAPFFYAPGNHDMSNAVMSEAWRARFGPSFYHFLYKDVLFLVVNSELFGMVTNP
                     WVSVPGPWTQEEQMSYIERVLSRHADVRWTIVITHQPLWDVAEVHPDWLKVEALLGER
                     PYTVFAGHAHRYQRSLRHDRKYITLATTGGASPLRGALYGEFDHVAMVHMSSRGPTIA
                     NLMLDGIQPEDVTTEDSLRALRQLSDAVQVETEYLDGDLFTSGAVRYRIVNSGSAPLE
                     ATPIVLGNDVLRAQATPQAISVPPGGERRVALNLKSTHALAFESLPPARVTWSLRSML
                     LDRPVFVEIETALLPQRHFPLERISRLVRLDGDLEEWHLPYSVTRQGDIASPQTDPAD
                     ISFRFGIGYDDENLYLAAEVIDD"
     gene            complement(1592..1855)
                     /locus_tag="AC16UZ_05165"
     CDS             complement(1592..1855)
                     /locus_tag="AC16UZ_05165"
                     /inference="COORDINATES: protein motif:HMM:NF014609.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR6246106.1"
                     /translation="MQLTIVDVFAERRYAGNQLAVVRDASGLSTAEMQDIAREMNFSE
                     TAFVLESGSGHARARYFTPPPVRLGGEFDRALAGASSSIRAWR"
     gene            1985..2437
                     /locus_tag="AC16UZ_05170"
     CDS             1985..2437
                     /locus_tag="AC16UZ_05170"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR6246107.1"
                     /translation="MTGTVEGIYLAAKHGDSQRAVPAARLTIGVGLEGDRHANSKGGV
                     VTLIEAEAVERFIAETGLDITAADTGRNIATRGIGLNDLVGRQFRVGGVLMEGFELCE
                     PCATLGARLASRDVSAAAIVAAFTHSGGIRAFVRGTGTVAPGSAVEPA"
     gene            complement(2447..3037)
                     /locus_tag="AC16UZ_05175"
     CDS             complement(2447..3037)
                     /locus_tag="AC16UZ_05175"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6246108.1"
                     /translation="MTRQSGADGYPKQVRLPGGEEIEIRLMTGEDRDAVCEFARGLAE
                     EDLMFLRVDLTEPAVIDQWIRNLETGHSTSIVAYDGTGLVGYATVHRDPARWTRRVGE
                     IRVNVSPAYRAKGLGRTLTSQIFDLARSLGLRKLMANMTVDQSGAQAAFRRLGFVPEA
                     LLADYVEDRSGTPRDLVIMSYDIEGHTDQVAEPLRV"
     gene            complement(3057..4562)
                     /locus_tag="AC16UZ_05180"
     CDS             complement(3057..4562)
                     /locus_tag="AC16UZ_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR6246109.1"
                     /translation="MRLTSHDASFLYSESASGPMHTASIFVLEGELPFERVFSHFQAR
                     MHLIARYMQKLAFVPFNLAHPKWVDDPDFDLANHVVPHSVPEGSTLEEAVDLLLAVNE
                     GLLDRNRPLWKTYVVDGVPGKTLMLQQVHHAMIDGVSGIQLTMVQFDFDRDAPDPPPG
                     EPRQPATPPSAMDLVGEVVRESAERLSVNPLAGVHTDRKSFELLRNAGRAFSRFFTEP
                     AITAPWNAGLVGPKRRLRWTSHPFSEFREIRRAFGGTINDVVLAMVSEGAARYLDRRR
                     EETEGKYLRIMCPVSVRTEDEAGSLGNRVSAIFPLLPAWSMDAAERLRAVIGETERIK
                     RDEEAQALTFLSETMPSVPAVAMAPTLLVGTRFDPTALAGRMPLPVVPNLGTRIPLYG
                     FNFTCTNVPGVQVPQYLAGHEISNIIGILMLTGTLGYGMAVVSYNQNMRFNFVCEPRL
                     MPDLDVMVEAVEQVYQELLEEARRVNEPPAPPSRGGRSEKASRKSYDSPAA"
     gene            4700..>5176
                     /locus_tag="AC16UZ_05185"
     CDS             4700..>5176
                     /locus_tag="AC16UZ_05185"
                     /inference="COORDINATES: protein motif:HMM:NF014795.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR6246110.1"
                     /translation="MRGGQVFMEGLVAHGADCIFGNPGTTENSLLDRLIDYPGLRYYV
                     ALHEGVAVGAASFYAQASGKTGIVNLHVAPGLGNGIGMIYGALKANSPLIITAGQQDT
                     RMRMREPLLAHDLVAMAEPVCKWSAEPRSADEIGPMLRRAFKIANEPPRGPVFMSLP"
BASE COUNT          904 a         1729 c         1681 g          862 t
ORIGIN      
        1 gtcgtcgatt acctcggcag caaggtaaag gttctcgtcg tcgtagccga tcccgaagcg
       61 aaacgagatg tccgccggat cagtctgcgg gctggcaatg tcaccctggc gcgtcaccga
      121 gtacggcaga tgccattcct cgaggtctcc gtccaggcga accagacggc tgatgcgctc
      181 cagcggaaag tggcgctggg gaagcagcgc ggtctcgatc tctacgaata ccggccgatc
      241 gagcagcatc gaccgcaacg accaggtgac cctcgccggg ggcaggctct cgaaggcgag
      301 cgcgtgcgtg gatttcagat tcagcgcgac ccgccgctcc cctccgggcg gcaccgagat
      361 tgcctgggga gtcgcctggg cgcgcagaac gtcgtttccc aggacgatgg gcgtcgcctc
      421 gaggggagcc gaccccgagt tgacgatccg gtagcgaacc gcgcccgagg tgaacaggtc
      481 tccgtcgaga tactcggttt cgacctgcac cgcgtccgac agctgcctca acgcacgcag
      541 cgagtcctct gtggtgacgt cctcgggttg aatcccatcg agcatcagat tcgcgatggt
      601 gggtccgcgc gaggacatgt gcaccatcgc cacatgatca aactcgccgt aaagagcgcc
      661 ccggagcggg ctggccccgc cggtggtcgc cagcgtgatg tacttgcgat cgtggcgtaa
      721 cgatcgctgg tagcggtgcg cgtgaccggc aaacaccgta tacgggcgtt caccgaggag
      781 cgcttcgacc tttagccagt cggggtgaac ctcggcgacg tcccacagcg gctgatgggt
      841 gatgacgatc gtccagcgga cgtcggcatg cctggagagc acgcgctcga tgtaactcat
      901 ctgctcttcc tgggtccagg gccccggcac ggacacccac ggattcgtca ccatgccgaa
      961 cagctcggag ttgacgacca ggaacagcac gtccttgtag aggaagtgat agaaggacgg
     1021 tccgaaacgg gcgcgccagg cctcggacat gaccgcgttg gacatgtcgt gattgcccgg
     1081 cgcgtagaag aagggcgcat cgagctggtc gacaaaggcc tcgacttcat cccactcccc
     1141 cgtgagccgg gccggatcgt ccgtgtagcc ttcaatgaga tctcccacgc tgaccacgaa
     1201 tgccggctgc agcagattga ccttgggcat ggcggactcg aacacgcctg gccgatgacc
     1261 gcgcgtgcgg tcggccacca cgacaaaggt gaaatcctct tccgcatcgt ttgcctggag
     1321 tcccgtccac ggggtggctg aggattcaac gtgagcttcg atgcggctcg cgtggtcggc
     1381 agccagggtg actcccgagg agaacaacgc gacgagcaca accaggcccc tcgcgggcca
     1441 gctcgccctg accggcgggc ggctcgcgga cggccgtcct gtcttctgcc gacgcgaatc
     1501 tttcaagcca gtcggccctc tgccaccagt tgcaccttgc cgccgacgcg gatctcgtct
     1561 cccacgtcga gatagagccg cgagggccgt ttcatctcca cgccctgatc gacgacgacg
     1621 cgcccgccag ggcccggtcg aactccccgc ccagccgcac gggcgggggc gtgaaatagc
     1681 gggcgcgggc gtggccgctg ccgctttcca ggacgaaggc ggtctccgag aaattcatct
     1741 cccgggcaat gtcctgcatc tctgcggtgg agaggccgct cgcatctcgc accaccgcca
     1801 gctgattacc ggcgtagcgg cgctcggcga acacgtcgac gatggtgagc tgcattcgag
     1861 aacccaaggc ggcacgtttc gccatcgaga ctagcacccg ctctctgcat ttcccaacct
     1921 ccgcccatca ggccgcgtga tgaacttgcg ctaaactcgc tcatcagcac cggagggctt
     1981 cccgatgaca ggcacagtcg agggcatata tctggccgca aagcacggcg attcccagcg
     2041 ggccgtgccc gcggctcgac tgaccatagg cgtcggcctc gagggtgatc gtcacgccaa
     2101 ctcgaaaggc ggggtggtca ccctgataga ggccgaggcg gtagaacggt tcatcgccga
     2161 gacgggcctg gacatcacgg cggcggacac cggtcgaaac atcgccaccc gcggcatcgg
     2221 cctgaacgac ctggtgggca ggcagtttcg cgtcggcggg gtgctgatgg aaggcttcga
     2281 gctatgcgag ccctgcgcca ccctgggcgc gcggctggcg agtcgggacg tgagcgcggc
     2341 ggccatcgtc gctgccttta cgcacagcgg gggcattcgc gccttcgttc ggggaaccgg
     2401 caccgtggca ccgggaagcg ccgtcgagcc ggcctgatcg catctctcac actcgcagcg
     2461 gttcggcaac ctgatccgtg tgaccctcga tgtcgtagga catgatcacc aggtcccgcg
     2521 gggttccgct acggtcctcc acgtagtccg ccagcagcgc ctcgggcaca aagcccagcc
     2581 gacgaaaggc cgcctgggca ccggactgat ccaccgtcat gttcgccatg agctttcgca
     2641 agccgagcga ccgggccagg tcgaaaatct gcgacgtcag cgtccggccc agccccttgg
     2701 cgcggtatgc ggggcttacg ttcacccgga tctcccccac tctgcgggtc catcgggcgg
     2761 ggtcgcgatg cacggtcgcg taccccacca ggccggtgcc gtcgtaagcc acgatcgaag
     2821 tcgagtgacc cgtctcgagg ttgcggatcc actgatcgat caccgcgggc tccgtcaggt
     2881 ccacgcgcag aaacatcagg tcttcctcgg caagcccccg ggcaaattcg caaaccgcgt
     2941 cccggtcctc tccggtcatc aggcggatct cgatctcttc gccgccgggc agccgcacct
     3001 gcttggggta cccgtccgca ccgctctgtc tggtcatctt tctacaccct cctcagtcag
     3061 gccgccggcg agtcgtagga cttccggctt gccttttccg accggccgcc ccggctgggc
     3121 ggggccggcg gctcgttgac ccggcgcgct tcctcgagca gctcctgata cacctgctcg
     3181 acagcctcca ccatcacgtc cagatcgggc atcaggcgcg gctcgcagac gaaattgaag
     3241 cgcatattct ggttgtagct cacgactgcc atgccgtacc cgagggtgcc ggtgagcatc
     3301 agtatgccga tgatgttgct gatctcgtga ccggccaggt actgcggcac ctgaacgccc
     3361 ggcacgttgg tgcaggtgaa gttgaacccg tagagcggga tccgggtgcc caggttgggc
     3421 acgaccggca acggcattcg tccagcgagg gcggttgggt cgaagcgggt tcccaccagc
     3481 agcgttggcg ccatggcgac cgcaggcacg gacggcatcg tctcgctgag gaacgtcagc
     3541 gcttgcgcct cctcgtcccg tttgatgcgt tcggtctctc caatcacagc gcgcagcctc
     3601 tcggcggcgt ccatggacca ggccggcagc agcgggaaaa tggcggacac acgattgccg
     3661 aggctgccgg cttcgtcctc ggtgcgcacg ctcaccgggc acatgattct caggtacttc
     3721 ccttcggttt cctcgcggcg gcgatccagg tagcgggcgg ccccctctga gaccatggcg
     3781 agaaccacat cattgatggt cccaccgaag gcccgtctga tctctctgaa ctcggagaac
     3841 gggtggctgg tccagcggag ccggcgcttc ggcccgacca ggccggcgtt ccaaggcgcc
     3901 gtgatggcgg gctcagtgaa gaagcgcgag aacgcgcgac cggcattgcg caggagctcg
     3961 aagctcttgc ggtcggtgtg cacgcctgcg agcgggttga ccgacagccg ctcggcgctc
     4021 tcccgcacca cctcgccaac gagatccatg gcagagggcg gcgttgcagg ctgccgcggt
     4081 tctccgggcg gaggatccgg ggcgtcccga tcaaagtcga actggaccat ggtgagctgg
     4141 atgccggaaa ccccgtcgat catggcgtga tgcacctgct gaagcatcag cgttttcccc
     4201 ggcacgccgt ccaccacata ggtcttccaa agcggtcggt tgcggtcgag cagcccctcg
     4261 ttcaccgcga gcaggagatc caccgcctcc tccagggtcg agccctcggg aaccgagtgc
     4321 ggcaccacgt gattggcgag atcgaagtcc gggtcgtcca cccacttcgg gtgcgccagg
     4381 ttgaatggta cgaaggccag cttctgcatg taacgcgcaa tgaggtgcat gcgggcctga
     4441 aagtgactga acacacgctc gaacggcagc tcgccctcca gcacgaagat ggaagcggtg
     4501 tgcatcgggc cgctggccga ttccgagtac agaaacgagg cgtcgtgcga agtcagtcgc
     4561 atgggttcat ccgatcaggg gcgcacacta tgcgcggaga cgaaccggcc cgtcaacgcg
     4621 acgccgcggc tgggcggcct ggctaccggc gctgcgctgc ctgtaacatt cgacgctcgg
     4681 tttacccagg gagaaggcga tgcgcggtgg tcaggtgttc atggaggggc tcgtcgccca
     4741 cggcgcggac tgcatcttcg ggaaccccgg caccaccgaa aattctctgc tcgaccggct
     4801 catcgactac ccggggctgc gctactacgt cgccctgcac gagggcgttg cggtgggcgc
     4861 cgcgagcttc tacgcccagg cgagcggcaa gacgggcatc gtgaacctgc acgtcgctcc
     4921 cgggctcggc aacggcatcg ggatgatcta cggcgcgctg aaagccaact cgccgctcat
     4981 catcaccgcg gggcagcagg acacccgcat gcgcatgcgg gaacctctgc tcgctcacga
     5041 cctcgtcgcc atggccgagc cggtctgcaa gtggagcgcc gagccacgca gcgccgacga
     5101 gatcggcccc atgcttcggc gggcgttcaa gatcgcgaac gagccgccgc ggggcccggt
     5161 tttcatgtcc ctgccg
//
LOCUS       DBNHOX010000234         3982 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1368983, whole genome shotgun sequence.
ACCESSION   DBNHOX010000234 DBNHOX010000000
VERSION     DBNHOX010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1368983"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="AC16UZ_05190"
     CDS             complement(<1..317)
                     /locus_tag="AC16UZ_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6246111.1"
                     /translation="MEVSDETLVQQVVRLKDLQAFERLVQRHEKRILHLQRRFIRDHA
                     LAEDLCQETFLRAWDRLETFSGRGSFAAWLTKLAYNIFLQHRRKHAARTSRETPYEEE
                     AEAG"
     gene            complement(336..698)
                     /locus_tag="AC16UZ_05195"
     CDS             complement(336..698)
                     /locus_tag="AC16UZ_05195"
                     /inference="COORDINATES: protein motif:HMM:NF039701.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HIR6246112.1"
                     /translation="MEDILVPISLFLVIGAVISLSFYFRYRTRQEIQSTVRAAIERGQ
                     ELTPEVLDGLSDALNSRHGDLRRGIISLAIGIALFLFAGLLGEPDAEGPLMAISAFPL
                     LLGIGYLAMWFFLRRKAG"
     gene            919..1494
                     /locus_tag="AC16UZ_05200"
     CDS             919..1494
                     /locus_tag="AC16UZ_05200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6246113.1"
                     /translation="MADQDRKKWDQRYAAGAYADRPHPSAILADWLPRLSAGDALDIA
                     CGAGRNALHMARAGFRVDAVDVSAEALARAASAAAEAGLEVNWMQRDLDTGLPVEGPY
                     DLIVMIRYVDESLLRALPALLRPGGVLIVEEHLTTEEPVIGPRDARFRVMPGALRAAV
                     PDLEVLEATDTVTDDPDGRRVALARLVARKP"
     gene            complement(1508..2362)
                     /locus_tag="AC16UZ_05205"
     CDS             complement(1508..2362)
                     /locus_tag="AC16UZ_05205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6246114.1"
                     /translation="MKIGLSNFPTEYSISAVALARAAEERGFESLWVVEHTHIPASRR
                     TPYPIGGDLPSIYWESYEPFTFLAQAAAVTTDLRVGTGICLVPEHHPIALAKRVASLD
                     ALSGGRFLFGVGAGWNAEELENHGVAFGDRWKVLREHVLAMKACWTEKDAEYHGRFVD
                     FDPVWVEPKPVQKPHPPIYIGASSTWAMERIVEYGDGWLPVPVPEFDERLALLNRLCE
                     ERGRDRSEIDVCLMTVVDSADTLAGIRDKGVNRVILSLPTLGEDEALRVLDGYANVVE
                     WAKEIGDG"
     gene            complement(2404..>3982)
                     /gene="ggt"
                     /locus_tag="AC16UZ_05210"
     CDS             complement(2404..>3982)
                     /gene="ggt"
                     /locus_tag="AC16UZ_05210"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR6246115.1"
                     /translation="AIASDVGARVLRAGGNAIDAAVATAFALAVTYPEAGNIGGGGFL
                     VYQPQEGGADAYDFRETAPTGSHPQMWLDEDGEYSPARQYVGPVAAGVPGTVAGLHKA
                     WRDHGSLPWAELVAPAIGLARDGFAIPEDLARSIAATLPQMEAYPASLAQFSRDGAPM
                     APGELLVQRDLARTLELIAADGPDAFYRGPIAEQIVALMASTGGLITAGDLASYRAVK
                     RKPIRGTYRGYEIVSMPPPSSGGVALVQTLNLLEGYDIAGEGFGSARTLHLITEALRR
                     TFADRARYLADPDFVKDMPVRRLTSKSYAAELRETISLNRAGTSSPETFEWPTASNDT
                     AHFSVVDAQRNAVSLTYTLNGRYGNQMVVPGAGFLLNNEMGDFNPVPGLTDERGRIGT
                     TPNLAAPGKRMLSSMSPTIVLKDGELFMVTGARGGRSIISTVLQTIMNVVDHGMNAQA
                     AVDARRIHHQWLPDRLRYEPQSFSRDTLNALRWLGHEIEQGGASWSNAEVIVVANDGW
                     LEGGDDRRRSRGSVAGY"
BASE COUNT          629 a         1302 c         1368 g          683 t
ORIGIN      
        1 cctgcctcag cctcttcctc gtatggggtt tcacggctcg ttctggcggc atgctttctg
       61 cggtgctgca gaaagatgtt gtaggccagc ttggtgagcc aggcagcgaa actgccccgc
      121 ccgctgaagg tttccagccg gtcccacgcc cggagaaacg tttcctggca gaggtcctcc
      181 gccagggcgt ggtccctgat gaagcggcgc tgaaggtgca ggatgcgctt ctcgtgccgc
      241 tggaccaggc gctcgaacgc ctgcagatcc ttcagccgaa cgacctgctg taccagggtt
      301 tcgtcgctga cctccatcac gccattagac cactgtcatc cggctttgcg gcgcaggaag
      361 aaccacatcg cgagatagcc gatccccagc agcagcggga aggcagaaat ggccatcagg
      421 ggaccctccg cgtccggctc gccgagaagc cctgcaaaaa ggaaaagcgc gatgccgatg
      481 gccagcgaaa tgattccgcg acgcaggtcg ccgtggcggg agttcagggc atcggagagg
      541 ccgtcgagca cctcgggcgt caactcctgg ccgcgctcga tggccgcccg gaccgtgctc
      601 tggatttcct ggcgggtccg gtatcggaag taaaaggaca gtgagatgac ggctcctatg
      661 acgaggaaca gcgagatggg cacgagaatg tcttccacga tctgtctccg taattttctg
      721 tggctctaac tggactgatg cacgttgtcc gcttccggtt gcatgcctgg agcgaaaagt
      781 cggagacggt ggggggagat gaaggatgcc ccgggaccga ggtggggaga ggggagggcc
      841 ggttcccggg gcgtccaggg tgaaatgtgc cccggacgct gtgaaaatca cgtgaacgcc
      901 cgatcacctg gagagcccgt ggcggatcag gaccggaaga agtgggatca gcgctatgcg
      961 gccggcgctt acgccgatcg tccgcacccc agcgccatcc tcgcggattg gctgccccgg
     1021 ctttccgccg gtgatgctct ggacatcgcc tgcggggcgg gccgcaacgc cctgcacatg
     1081 gctcgcgctg gttttcgggt cgatgccgtt gatgtctccg cagaggctct ggccagggca
     1141 gcctcggcgg ccgccgaggc cgggctcgag gtgaactgga tgcagcgcga cctggacacc
     1201 ggcctgcccg tggaggggcc ctacgacctc atcgtcatga ttcgctacgt cgacgagtcg
     1261 ctgctacgag cgctgccggc attgctgcgc cctggcgggg tcctgatcgt cgaggagcat
     1321 ctgaccaccg aagagccggt gattggtccg cgggatgctc gctttcgggt gatgccgggc
     1381 gcgctgcgcg ccgcggtgcc cgatctcgag gtgctcgagg caacggacac ggttaccgac
     1441 gaccccgatg ggcgcagggt ggccctcgcc cggctggttg ccagaaagcc ctgagcaacc
     1501 ggccggctca gccgtcgccg atctccttcg cccactcaac gacgttggca tagccgtcca
     1561 ggacgcgcag cgcctcgtcc tcgcccagcg tgggcagaga caggattacc cggttgacgc
     1621 ctttgtcccg gatgccggcc agggtgtcgg cgctgtccac cacggtcatc aggcagacgt
     1681 cgatctcgct gcggtcacgg ccgcgttcct cgcagagccg gttcagcaga gcaaggcgct
     1741 cgtcgaactc gggtaccggg accggcagcc agccgtcgcc gtattccacg atccgctcca
     1801 tggcccaggt ggaggacgcc ccgatgtata tcggcggatg gggcttctgc actggcttgg
     1861 gttccaccca gacgggatcg aagtcgacga acctgccgtg gtactcggca tctttctcgg
     1921 tccagcatgc cttcatggcc aggacgtgct ctcgcagcac cttccagcgg tcgccgaacg
     1981 ccacgccgtg gttctccagc tcctcggcgt tccatcccgc gcccacgccg aacaggaagc
     2041 gtccgcccga cagggcgtcc agggacgcca cccgttttgc cagggcgatg gggtgatgct
     2101 ccgggaccag gcagataccg gtgccgaccc gcaggtcggt ggtcacggcg gctgcctggg
     2161 cgaggaacgt gaacggctcg tagctttccc agtagatgct cggcaggtcg ccgcctattg
     2221 ggtagggcgt ccgccgggag gcgggtatat gggtgtgctc caccacccac aaggactcga
     2281 agccgcgttc ctcggccgct cgggcaaggg ccacggcgga gatggaatat tcggtgggaa
     2341 agttggacag cccgatcttc atgtcgttgc tcctcattga gttgaaacgg tcgccgcgtg
     2401 cgctcagtac ccggccacgg acccgcggga gcgtcggcgg tcgtcgccgc cctccagcca
     2461 gccgtcgttc gctaccacta tgacctcggc gttgctccag gatgccccgc cctgctctat
     2521 ctcgtggccc agccagcgca gcgcgttcag cgtgtcgcgc gaaaagctct gcggctcgta
     2581 gcgcagtcgg tccggcagcc actgatgatg aattcgacgg gcgtccaccg ccgcctgggc
     2641 gttcatgccg tggtcgacca cgttcatgat ggtctgcagc acggtgctga tgatggagcg
     2701 tccgccccgc gctcctgtca ccatgaagag ctcgccgtcc ttcaacacga tcgtcggcga
     2761 catgctcgag agcatgcgct tgccgggtgc cgcgaggttg ggtgtggtgc cgatgcgccc
     2821 gcgctcgtcg gtcagcccgg gcaccggatt gaaatcgccc atctcgttgt tcagcagaaa
     2881 gccggcgcct ggcaccacca tctggttgcc gtaccggccg ttgagcgtgt aggtcagcga
     2941 cacggcgttg cgctgggcgt ccacgactga gaaatgggcc gtatcgttgc tggccgtcgg
     3001 ccactcgaag gtctccggtg aggacgtgcc tgcgcggttc agcgagatcg tttcgcgcag
     3061 ctcggcggcg taggacttcg atgtcaggcg ccgcacgggc atgtccttga cgaagtccgg
     3121 gtcggccaga taccgcgcgc gatcggcgaa cgtgcggcgc aacgcctcgg tgatcaggtg
     3181 cagagtgcgg gcggaaccga agccctcccc ggcgatgtcg tagccctcca gcaggttgag
     3241 cgtctgcacc agcgccacgc cgcccgagct gggcggcggc atggagacga tctcgtagcc
     3301 ccggtacgtg ccgcggatcg gcttgcgctt gacggctcgg taggacgcca gatccccggc
     3361 ggtgatcagg cccccggtgg aggccatcaa cgcgacgatc tgctcggcga tcgggccccg
     3421 gtagaatgcg tcgggaccat cggcggcgat gagttccagg gttcgggcca gatcgcgctg
     3481 caccaggagc tcaccgggcg ccatgggcgc gccgtctcgg ctgaactgag ccagggaggc
     3541 tgggtaggcc tccatctgcg gcagggtggc cgctatgctg cgggccaggt cctcggggat
     3601 ggcgaaaccg tcgcgggcga gcccgatggc gggcgccacc agctctgccc agggcaggga
     3661 cccgtgatcg cgccatgcct tgtgcaggcc ggcgaccgtt cccggcacgc cggcggccac
     3721 ggggccgacg tactgccgcg ccggcgagta ctcgccgtct tcgtccagcc acatctgcgg
     3781 gtgtgatccg gtgggcgcgg tctcccgaaa gtcgtaggcg tccgcgccgc cctcttgggg
     3841 ttggtacacc aggaaaccgc cgccgccgat gttgccggct tccggatagg tcactgccag
     3901 cgcaaatgcg gtcgccacgg cagcatcgat ggcgttgccg cccgcccgca gcacccgggc
     3961 gccgacgtcc gaggcgatgg cg
//
LOCUS       DBNHOX010000235         3279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_470192, whole genome shotgun sequence.
ACCESSION   DBNHOX010000235 DBNHOX010000000
VERSION     DBNHOX010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_470192"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="AC16UZ_05215"
     CDS             complement(<1..358)
                     /locus_tag="AC16UZ_05215"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246116.1"
                     /translation="MSVSLRGKNAIVTGAAVGIGPAYAEALAAEGVNVAVCDIRGEIL
                     ELPARLEAHGVRSVGWKADVSVPEDVRKVVDGTVEAFGGIDILISNAGKCWVSVADDD
                     LDKTIADYEGMVGTNLK"
     gene            complement(468..1481)
                     /locus_tag="AC16UZ_05220"
     CDS             complement(468..1481)
                     /locus_tag="AC16UZ_05220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6246117.1"
                     /translation="MGGKTINRQWLINGNPRGRAVALSDFVENEAELAPLGEGDVRVK
                     VEYLSFDPSQKGQLENIAGYSRGGAMGEVMYARGIGEVVESRSPGVAEGTKVAGFVGW
                     QEYASLPARSLEIVPDDDLLTARLGPLGGTGLTAYFGLLRVGRPEPGDMVVVSGAAGA
                     VGSMVGQIAEIAGCHTVGIAGGPEKCAWLVDEVGYDAAIDYKNENIKARLKELCPDGI
                     NVFFDNVGGEALNDALARIAPHARVVICGGISRYEQETLPPGPANYFNVVFRQANIEG
                     FLLSGYEAEYDVARERITEWIRSGAIVYKEDVQHGFENIPATLLRLFSGKNFGKQLLR
                     MGD"
     gene            complement(1566..3248)
                     /locus_tag="AC16UZ_05225"
     CDS             complement(1566..3248)
                     /locus_tag="AC16UZ_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6246118.1"
                     /translation="MTQLDEADWDLTVDLVVVGSGAAGMACALTAKLEGLDVLMVEKT
                     DRYGGTTALSGGVLWVPNNSLMKQAGVEDSAERAVTYLKHNIGNRVSAARLEAFVENA
                     PKMLDFLRARDCLEVAVFEGFPDYRPEDPGGCQGGRSVEPKVFAGRRLGDALGDLRAR
                     AAMAPAGIVGTMTELRRLAAVRSNPLELLKAWKVFPRNLWNRLSGAHHLANGRALIAW
                     LRHAMERTDVPLWLNAPLARIVTDDDGAVRGVLVRRGEEPVRVEARRGVVITAGGFEH
                     NGEMRSEYFGENTAEYSSGSPGNTGDAIRAGLDAGAAVDLMDDAWWAPTFMPPGQGPQ
                     IVIFERGKPGNIIVNAAGQRFANEADPYNEFVKHMQEADRSGASAIPSFLIFDDRYRS
                     KYPLGGMLPGVTPRRYLENGFVVRADTLVELAEIMGIDPAGLEDTVARFNDMASKGSD
                     DDYHRGDSAFDRFAGDPGVRPNPCLAALDEPPFYGMRIYPGDLGTKGGLLTNEHAQVL
                     RGDGTVVRGLYAAGNSSASAMGNFYPGAGGTIGPAMTFGYIAARHAAAEGSQ"
BASE COUNT          538 a         1171 c          998 g          572 t
ORIGIN      
        1 ctttgaggtt ggtccccacc atcccctcgt agtcggcgat cgtcttgtcc aggtcgtcgt
       61 cggccacgct gacccagcac ttgccggcgt tgctgatcag gatgtcgatg ccgccgaagg
      121 cttcgaccgt cccgtccacc accttgcgca cgtcctcggg cacggacacg tcggccttcc
      181 agcctacgga ccggacgcca tgggcctcca ggcgggcggg caactcgaga atctctccac
      241 gaatgtcgca aacggcgacg ttgacccctt cagcggcaag cgcttccgca taggccggac
      301 caatgccgac ggcggcgccc gtaacgatgg cgttcttgcc acgcagtgaa actgacatca
      361 tcccccctca tcgagatgtg attgaactcc cgccacgaga atacacgaag gcttcagtct
      421 gcgtcagcgt gccccaaccg gggatcgatc cggccggaga cggcacctca gtcgcccatc
      481 ctgagcagct gcttgccgaa gttcttgccg gagaacagcc gcagcagcgt cgccggaatg
      541 ttctcgaagc cgtgctggac gtcttccttg tatacgatgg ccccggaacg gatccattcg
      601 gtgatgcgtt ccctggcgac gtcgtactcg gcttcgtagc cgctcagcag aaagccttcg
      661 atgttggcct gcctgaacac gacgttgaaa tagttggcag gacccggcgg cagggtttcc
      721 tgctcgtacc gggatatgcc gccgcagatg acgactctgg cgtggggcgc gatccgcgcc
      781 agcgcatcgt tgagcgcctc cccgccgacg ttgtcgaaga acacgttgat cccgtccggg
      841 cacagctcct tcaggcgggc tttgatgttc tcgttcttgt agtcgatggc ggcgtcgtag
      901 ccgacctcgt ccacgagcca tgcgcacttt tccgggccgc ccgcgatccc aacggtgtgg
      961 cagccggcaa tttcggctat ctgaccgacc atcgaaccca cggccccggc cgcacccgac
     1021 accaccacca tgtcgccggg ttccggccgg cccacccgaa gcagcccgaa gtaggcggtg
     1081 aggccggtgc cgcccagcgg accgagccgg gcggtgagca gatcatcgtc gggcacgatc
     1141 tccaggctgc gagccggcag cgacgcgtac tcctgccagc ctacgaaccc ggcaaccttc
     1201 gtgccctccg ccacgcccgg gctgcgcgac tccaccacct cgccaatgcc cctggcatac
     1261 atcacctcgc ccatggcgcc tccccgggaa tagccggcga tgttctccag ctgccccttc
     1321 tgggacgggt cgaagctcag gtactccacc tttacccgga cgtcgccttc tcccaggggc
     1381 gcaagctccg cctcgttctc cacgaagtcg ctcagcgcca cggctctgcc gcgcgggttt
     1441 ccattgatga gccactgtct gttgatggtc ttcccaccca tttgctttcc cctgacatca
     1501 aatccgtctg cgcgacgggc cccacccgca gcgcccccgc gccgtcacga tggtacttta
     1561 accgcctact ggctaccctc ggcggccgcg tgccgggcgg cgatgtaacc aaaggtcatc
     1621 gccggcccga tggtcccgcc ggcaccgggg tagaagttac ccatggccga ggccgagctg
     1681 ttccccgcgg catacaggcc gcgaaccacg gtgccgtcgc cacgcagaac ctgagcgtgc
     1741 tcgttggtga gcaacccgcc tttggtaccg agatcgcccg ggtaaatccg catgccgtag
     1801 aagggcggct cgtccagggc agccaggcag gggttgggtc gtaccccggg atcaccggcg
     1861 aagcggtcga acgcgctatc gccccggtga tagtcgtcgt cgctgccctt cgaggccatg
     1921 tcgttgaacc tggccaccgt atcttcgaga ccggccgggt cgatccccat gatttcggcc
     1981 agctcgacca gggtgtccgc ccgcacgacg aacccgtttt ccagatatcg ccgaggcgtg
     2041 acgccaggga gcatgccgcc caggggatac ttgctccggt aacggtcgtc gaagatgaga
     2101 aacgacggga tggcggaagc cccgctgcga tccgcctcct gcatgtgctt gacgaactcg
     2161 ttgtagggat cggcctcgtt ggcaaagcgc tggcccgccg cgttcacgat gatgttaccc
     2221 ggcttgccgc gctcgaaaat gacgatctgc ggtccctggc caggcggcat gaaggtgggc
     2281 gcccaccagg cgtcgtccat gaggtctacc gcggcgccgg cgtcgagacc tgcgcggatg
     2341 gcgtctcccg tgttgccagg cgagccggat gagtattcgg ccgtgttctc gccgaagtac
     2401 tccgagcgca tctcgccgtt gtgctcgaac cctcccgccg tgatcaccac gccgcgcctg
     2461 gcttccaccc gcacgggctc ttcgccacgc ctgaccagca cgcctcgaac ggcaccgtca
     2521 tcgtcggtga cgatgcgcgc aagcggcgcg ttcagccaca gcggaacgtc cgtgcgttcc
     2581 atggcgtggc gaagccatgc gatgagggcg cgcccgttgg caaggtgatg ggctccggag
     2641 agacggttcc acaggttccg cgggaagact ttccaggcct tgagcagctc cagcgggttg
     2701 gagcgcacgg cggcgaggcg cctcagctcc gtcatggtgc cgacgatgcc tgcgggcgcc
     2761 atggcggcgc gggcgcgcag gtcgcccagc gcatcgccca gcctgcggcc ggcgaagacc
     2821 ttcggctcta ccgaccggcc gccctgacag ccgcccggat cttccggccg gtaatcggga
     2881 aaaccttcga aaacggccac ttccagacaa tcgcgcgcgc ggagaaagtc gagcatcttc
     2941 ggcgcgttct caacgaacgc ctcgagcctg gccgcgctga cccggttgcc gatgttgtgc
     3001 ttcagatacg tcacggcacg ctcggccgag tcctcgacgc ccgcctgctt catcagcgag
     3061 ttgttgggca cccacagcac gccaccggaa agcgccgtgg tgccgccgta acggtccgtc
     3121 ttctcgacca tcagaacatc caggccctcg agctttgccg tcaaggcaca ggccatcccc
     3181 gccgccccgg accccaccac cacgagatcg acggtgaggt cccagtcagc ctcgtcgagt
     3241 tgcgtcatcg ctgcctccct ctatcgcccg ctggccgtc
//
LOCUS       DBNHOX010000236         2358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_919706, whole genome shotgun sequence.
ACCESSION   DBNHOX010000236 DBNHOX010000000
VERSION     DBNHOX010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_919706"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1504)
                     /locus_tag="AC16UZ_05230"
     CDS             complement(<1..1504)
                     /locus_tag="AC16UZ_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6246119.1"
                     /translation="MWLSDISVQRPVFASVISLALIAFGALSFNLLPLREYPDINPPL
                     VSISTAYVGASAEVIESRVTQVIEDQISGIEGIKSISSSSRDEASNINIEFELNRNID
                     EAANDVRDRVARIVDRLPDDVELPIVAKQDSDARPVMYISLTSSRMSVMELNDYAERY
                     IVDRFEIIPGVASAAMHGSGKPSMRIWLDRLALAARDLTVIDIEDALRRENLELPAGR
                     IESAEREFRVRLARGYQSDEDFRQLVLGVGGDGHLIRLGEVADVNLAPSNTRESFRAN
                     RQNTVGLAITKQSTANTLATLEAVKAEIARVNEALPDHMEFVPSSDDSLFIREAINAV
                     YQTIAITTVLVSLVIFVFLGSIRSMIIPAVTIPVCLTSAFIALALFGYSVNLVTILAL
                     VLCIGLVVDDAIVVLENIHRRVERGEPPLVAAYRGSRQVAFAVIATTVVLVAVFTPIM
                     FLRDNMGVIFSERAVTVSAAVVFSSVLALSLTPMMSSKLLRKTTAKNPVTR"
     gene            complement(1510..>2358)
                     /locus_tag="AC16UZ_05235"
     CDS             complement(1510..>2358)
                     /locus_tag="AC16UZ_05235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6246120.1"
                     /translation="TAQVTDSVSQVRFEDGDYVNAGDVLVELTNEEETALLAEAEANA
                     RDTRTQLDRLEDLVLQGSVPISQVDEARARHSAAVARYQSVVARLEDRLITAPFSGVL
                     GFRQVSTGTLITPGTPITTLDDVSVIKLDFSIPESHLSRLQPGQRIEAESPAYSGSVF
                     DATVSTIGSRVDPVTRAAVVRAHIDNGDMRLRPGMLLTARLTTSEREVLMIPEAALLQ
                     RGTQVRVFTEVDGTARSRLITHGVRDAGWVEVREGLTEGERVITDGLIKVRDGSPITT
                     VRAEQG"
BASE COUNT          413 a          732 c          791 g          422 t
ORIGIN      
        1 tccgggttac cgggttcttt gccgtcgttt tgcgtagaag cttggagctc atcatgggcg
       61 tcagcgacag ggccaggacg ctggagaaga ccacggcggc ggaaaccgtg acagcccgct
      121 cgctgaatat cacgcccatg ttgtcgcgca ggaacatgat gggcgtgaac acggccacca
      181 gcacgacggt ggtcgcgatc accgcaaagg ccacctgcct cgaaccccgg tacgcggcga
      241 cgagcggagg ttcgccacgc tccacccggc gatggatgtt ctccagcacg acgatggcat
      301 cgtccacgac cagcccgatg cacagcacca gggccaggat ggtgaccagg ttgaccgagt
      361 aaccgaacag ggccagggcg atgaatgccg acgtcagaca cacggggatg gtcacggcgg
      421 ggatgatcat cgagcggatg ctgccgagaa agacgaagat cacgaggctg accagcaccg
      481 tggtgatggc gatggtctgg tacacggcgt tgatcgcctc gcgaatgaac agcgaatcgt
      541 ccgagctagg cacgaactcc atgtgatcag gcagcgcctc gttcacgcgc gcgatctcgg
      601 ctttgacggc ttccagcgtg gcgagcgtgt tggctgtgga ttgcttggtg atggccaggc
      661 caacggtgtt ctggcgattg gcccggaacg actctctggt gttgctgggc gcgaggttca
      721 cgtcggcgac ctcacccagg cgaatcagat gcccgtctcc acccacgccc agcaccagct
      781 gtcgaaaatc ctcgtcgctt tggtaacccc gggccaggcg cactcggaac tcgcgctctg
      841 cggactcgat gcgtccggcg ggaagctcga ggttctctcg ccgcagagca tcttcgatat
      901 cgatcacggt caggtcgcgg gcggccagcg caagccggtc cagccatatc cgcatgctcg
      961 gtttgccgct gccgtgcatg gccgcgctgg cgacgccggg aatgatttcg aagcggtcga
     1021 cgatgtagcg ctccgcgtag tcgttgagct ccatcacgct cattcgcgag ctggtcaggc
     1081 tgatatacat gacgggacgg gcgtccgagt cctgtttggc gacgatgggc agctcgacgt
     1141 cgtcaggcag ccgatcgacg atgcgggcca ccctgtcccg gacgtcgttg gccgcctcgt
     1201 ctatgttgcg gttgagctcg aactcgatgt tgatgttgga ggcctcgtca cggctcgacg
     1261 agctgatgct ctttatgccc tcgatgccgc tgatctgatc ctcgatcacc tgagtgacgc
     1321 gggattcgat gacctcggcc gacgcgccta cgtaagcggt gctgatggaa accagcgggg
     1381 ggttgatgtc gggatactcg cgcagaggga gcaggttgaa ggacagagcg ccgaacgcga
     1441 tcagggccag gctgatcacg ctggcgaaca ccggcctctg aacggagatg tcagaaagcc
     1501 acatgatgct cagccctgtt cggcgcgaac cgtcgtgatg ggagagccat cgcgcacctt
     1561 gatgaggccg tcggtgatga cgcgctcacc ttccgtcaag ccctcccgaa cctccaccca
     1621 gccggcgtcg cgtacgccgt gagtgatcag cctgctgcgg gcggtgccgt cgacctcggt
     1681 gaagacgcgc acttgcgtac cgcgctgaag cagcgctgcc tcgggaatca tgagcacctc
     1741 ccgctcggag gtggtcagcc tggccgtgag cagcatgccc gggcgcaggc gcatgtcccc
     1801 gttgtcgata tgcgcccgca ccacggcagc gcgcgtgacg gggtcgaccc gggagccgat
     1861 ggtgctcacc gtcgcgtcga acaccgaccc ggaataggcg gggctttccg cctctattcg
     1921 ctggccgggt tggaggcggc tcaggtgact ctccgggatg gagaagtcga gcttgatgac
     1981 ggagacgtcg tccagggtcg tgatcggggt tccgggggtg atcagcgtgc cggtgcttac
     2041 ctggcgaaac cccagaacac cggagaaggg cgcggtgatc agccggtcct cgagacgtgc
     2101 gaccaccgac tggtagcgcg cgacggcggc cgagtggcgc gcgcgagcct cgtccacctg
     2161 agagatcggc acgctgccct gaaggaccag gtcttccagc cggtccagct gggttcgggt
     2221 atccctggcg ttggcctctg cttctgccag cagagccgtt tcctcctcgt tggtgagctc
     2281 caccagcacg tcgcccgcgt tgacgtagtc gccgtcctcg aaacgcacct ggctgacgct
     2341 gtccgtgacc tgggcggt
//
LOCUS       DBNHOX010000237         5672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1369168, whole genome shotgun sequence.
ACCESSION   DBNHOX010000237 DBNHOX010000000
VERSION     DBNHOX010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1369168"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..692)
                     /locus_tag="AC16UZ_05240"
     CDS             complement(117..692)
                     /locus_tag="AC16UZ_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6246121.1"
                     /translation="MDPLAQLTRDRAEARRLEDPCANLCTVASVDSAGNPQARTLVLR
                     DVNERLAIFANETSPKWREISLNERISVVVWLPVLNLQYRLRCGTERVPTHLVHDRWH
                     QRPDPPKRLDWFYTKHLPQSSPLPDRDTLLERLAQIDLPEPLAPPQTAAGLYLSPTTV
                     ERLDLNQSNGIHDRRHFELSEDGWVQSVLVP"
     gene            complement(742..933)
                     /locus_tag="AC16UZ_05245"
     CDS             complement(742..933)
                     /locus_tag="AC16UZ_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246122.1"
                     /translation="MVFLCFIVLLGIAYLVWRVTDQMPDLIFRLSEVQRDVAEIRRRL
                     TEESDAPAGAAQGGGDAEA"
     gene            complement(1171..1665)
                     /locus_tag="AC16UZ_05250"
     CDS             complement(1171..1665)
                     /locus_tag="AC16UZ_05250"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SixA phosphatase family protein"
                     /protein_id="HIR6246123.1"
                     /translation="MKIWIIRHAKSSWAQPGLSDFDRPLNDRGRADGPRMAAWLASQD
                     APAEWIWTSDAARARATAAFVERGFSAARPLVVEEHALYHASARGALDVLRGTPEAVA
                     SVALVAHNPGLTDLVNLLAGNLVTENLPTFGVARFDVAAPWHSVGEQRADLEILTAPK
                     EIPS"
     gene            complement(1677..2039)
                     /locus_tag="AC16UZ_05255"
     CDS             complement(1677..2039)
                     /locus_tag="AC16UZ_05255"
                     /inference="COORDINATES: protein motif:HMM:NF025178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR6246124.1"
                     /translation="MSEAKQINLGPLSDLEEGDKRSFDAEGTQVLVCRVRGELYAIED
                     LCSHANKPLCRGRLSGYMITCPVHSARFDIRDGAHQGPPAFTGIRTFPIEEGADGAIV
                     TVTPKPLPAGFGGPLMTR"
     gene            complement(2048..3382)
                     /locus_tag="AC16UZ_05260"
     CDS             complement(2048..3382)
                     /locus_tag="AC16UZ_05260"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6246125.1"
                     /translation="MSSIRQMRGIDRTKATEPELGSNPINGDRYRSREFAELEWEHVW
                     TRVWLIGGLVHQIPEPGDFFTTDIGRESILFVRGDDGSIRAFYNVCQHRGNQLVQAEQ
                     GSLSGGEFQCAYHGWRFDAQGVLTWVYCEEDFPQGNPCGKRNLIEIPCDTWAGFIWYN
                     MDPDCKPLADFLDPVARQLDNYRMEDMRRTHWVTLEGNFNWKCVQDNFNESYHLPYVH
                     PQSCISLDEHYSSCQFDLYPSGHCRMFMPGGGPNSNYQGSPEAVFERLGQDLAFWEID
                     PEPFRDDLAGLRTALQQQKRKLAQKKGYDFSRYSDEQLTDNYHYTLFPNLSFSMKPDG
                     CIFLRGNPHPTDPEKCLFDMWYLTLFPEGAKEYYSNSMRDWVSVETPAAHERGKVGEV
                     SCGPGIDQDVAIWSTQQKGLTSRGFRGEYMPTQERRIRFFHENIDRYINGQL"
     gene            complement(3485..4393)
                     /locus_tag="AC16UZ_05265"
     CDS             complement(3485..4393)
                     /locus_tag="AC16UZ_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6246126.1"
                     /translation="MSDRRPAVSLAAVPGRRNTIVELAAEIERRGFAGIYCPSMGDGL
                     ALCEAIAYNTRQIEFGTSITPIYTRHANDFASTAAFIHEISNGRFRFGLGVSHAPAME
                     RLGVRQGKPLSDMRQFVSDLRAAPRVGELPPVVLATLRDRMIGLAAEVAEGMVFANAA
                     RGHMQHSLNVLGDERRADPGFFIGNMIPTCISDDVEAAKAVNRRTLTGYAMLPNYRNY
                     WKEAGFVEEMGAVEKALEAGEPDRIPDCLSDAWLAETTLFGPANAVRDGIEAWFDAGV
                     RTPIIVPSSAVGNQLKAFEEFFEIWS"
     gene            complement(4502..5263)
                     /locus_tag="AC16UZ_05270"
     CDS             complement(4502..5263)
                     /locus_tag="AC16UZ_05270"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6246127.1"
                     /translation="MFPLRDENPRVHPPYATVAIIILNVLVWVAVQGFGNHYALAESL
                     CLYGLIPGDLLGNAVPGTSIPLGSNLVCQLDGTANPASLFTSMFMHGGWFHIIGNMWF
                     LWVFGDNVEDVMGPFRFVVFYVVCGLAAAFAQIATNTSSLVPMVGASGAIGGVMGAYA
                     LMFPRAHVHTLIFLGFYVTTVAVPAVFMLGYWFLLQLLQGLPAMGSSQGGVAFWAHIG
                     GFLAGVALIHLFRRPNHLAQHLAQPERRSSRHRWF"
     gene            complement(5383..>5672)
                     /locus_tag="AC16UZ_05275"
     CDS             complement(5383..>5672)
                     /locus_tag="AC16UZ_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246128.1"
                     /translation="LDLAGRGAVAFFDMVSRDGLPRYLDFSEQTLYRRQDEPFASVPL
                     GFSPFPMPVDGRDMHPVWQMVVGDIEAFKAAKREALEPGDLPTADKISLTF"
BASE COUNT          979 a         1760 c         1919 g         1014 t
ORIGIN      
        1 gccgccttca ttcgaggctt catcgacgtt gcgaagcaga cgtgcttcca gaacctggtc
       61 ggtatcgtca ccgcggacgt ctgacaggat cgacgacctg gtcgcggtgg cggtgatcag
      121 ggaaccagca ccgactgcac ccagccgtct tccgacagct cgaagtgccg gcggtcgtga
      181 atgccgttgc tctgattgag atccaggcgt tcgaccgtcg tcggcgacag gtagagtccg
      241 gccgccgtct gcggaggtgc cagcggctcg ggaagatcga tctgggccag gcgctccagc
      301 agcgtgtccc ggtcgggcag tggggaactc tgcggaaggt gtttggtgta gaaccagtcc
      361 agccgcttgg gcggatccgg gcgctggtgc cagcggtcat gcacgaggtg cgtcggcacg
      421 cgctcggtgc cgcaccgcag gcggtattgc aggttcagga ccggcagcca gaccaccacc
      481 gaaatccgct cgtttaagct gatttcccgc catttggggc tcgtctcgtt tgcgaaaatc
      541 gccaggcgtt cgttgacgtc gcgcagcacc agcgtccgtg cctggggatt tcctgcggag
      601 tcgacggaag ccaccgtgca gaggttggca caggggtctt ccaggcgcct cgcctccgcg
      661 cggtcgcggg tgagttgggc aagcgggtcc actaaaagcc cggtggtgct tcgttcgatg
      721 gcgggcgccg gcttgccgac gtcaggcctc ggcgtcgcct ccgccctgag cggccccggc
      781 cggtgcgtcg ctctcctcgg tcaggcggcg gcggatctcc gcgacgtcgc gctgcacttc
      841 gctcaggcgg aaaatcaggt cgggcatctg atcggtgacg cgccacacga ggtaggcgat
      901 gcccagcagc acgataaagc aaaggaagac catgcacatc tccggttggt tgggggactc
      961 gcgattaggt ataccgactc catcgccggg agtccagcgg atgaacgtga acgcggctgc
     1021 cctttgggtt tccagcgcaa ccgtttcacc gctcgtatgc agcgtgggct gattgcctcc
     1081 gcgtcgcggg tcatgaaatc tggtgcgcta tcgagtcgtt cccgtcgagc tctgcaacgg
     1141 ttgcttcctg ttcaggatcg acaaaccagg ctaggacgga atttcctttg gcgccgtcag
     1201 gatttccaga tctgcgcgct gctcccctac gctatgccat ggggccgcga cgtcgaagcg
     1261 cgccacgccg aaggtcggca gattctccgt caccaggttg ccggccagca ggttgaccag
     1321 gtcggtgaga ccagggttgt gggccaccag cgccacgctg gcaaccgctt ccggagtgcc
     1381 tctcagcacg tccaaggcgc cgcgcgcgct ggcgtgatac agggcgtgct cctctacgac
     1441 cagcgggcgt gcggccgaga agccccgctc gacgaaagct gcggtggctc gggcccttgc
     1501 ggcgtcgctg gtccagatcc actctgcggg cgcgtcctgg cttgcgagcc aggcggccat
     1561 gcggggcccg tcggcccggc cgcggtcatt gagtggccga tcgaagtcgg acagacctgg
     1621 ctgcgcccat gatgacttgg cgtgacggat gatccagatc ttcacggggc cggcgttcag
     1681 cgggtcatca aggggccgcc gaagccggca ggaagcggtt tcggcgtcac cgtcacgatc
     1741 gcgccgtccg ctccctcctc gatgggaaac gtgcgaatgc cggtgaacgc cggtggcccc
     1801 tgatgggcgc catctctgat gtcgaaacgg gcgctgtgga cggggcaggt gatcatgtag
     1861 cccgaaagcc tgccccggca gagcggcttg ttggcgtggc tgcagagatc ctcgatggcg
     1921 tagagctcgc ctcggacgcg gcagacgagg acctgcgtac cctccgcgtc gaaggagcgc
     1981 ttgtcgcctt cttccaggtc gctcagagga cccaggttga tttgctttgc ttcgctcatc
     2041 gcctcgctca cagctgcccg ttgatgtaac ggtcgatatt ctcgtggaaa aagcgtatgc
     2101 ggcgttcctg ggtgggcatg tactcgccgc ggaacccgcg agacgtcagg cctttctgct
     2161 gagtcgacca gatggcaacg tcctgatcga ttccgggccc gcaggacacc tcgccgacct
     2221 tgccgcgctc atgggcggcg ggggtctcga cggacaccca gtcgcgcatg gagttggagt
     2281 agtactcctt ggcgccctcc ggaaacaagg tcaggtacca catgtcgaac aggcacttct
     2341 ccggatcggt ggggtgtggg ttgccgcgca ggaagatgca cccgtcgggc ttcatgctga
     2401 acgagaggtt cggaaacagc gtgtagtggt agttgtccgt gagctgctcg tccgaatatc
     2461 gcgagaagtc gtagcccttc ttctgggcca gcttgcgctt ctgctgctgc agcgcggtcc
     2521 gcaaaccggc gaggtcgtct cggaagggtt ccgggtcgat ctcccagaac gccaggtcct
     2581 gaccgaggcg ctcgaacacg gcctccgggg agccctgata gttgctgttc ggcccgccac
     2641 cgggcatgaa catgcggcag tgaccgctgg ggtagagatc gaactggcag ctcgagtagt
     2701 gctcgtccag gctgatacac gattgcgggt gcacgtatgg caggtgatag ctctcgttga
     2761 aattgtcctg gacgcacttc cagttgaagt tgccctcgag ggtcacccag tgggttcggc
     2821 gcatgtcttc catacgatag ttgtcgagct gtcgggcaac cgggtcgaga aaatcggcga
     2881 gaggtttgca gtcggggtcc atgttgtacc agatgaaccc cgcccacgta tcgcacggga
     2941 tctcgatcag attgcgcttg ccgcaggggt tgccctgggg gaagtcctcc tcgcagtaga
     3001 cccatgtgag gacaccctga gcatcgaagc gccagccatg gtaggcgcac tggaactcgc
     3061 cgcctgacag ggatccctgc tccgcctgaa ccagctgatt gccgcgatgc tggcagacgt
     3121 tgtagaacgc gcggatgctg ccgtcgtcgc cgcgcacgaa caggatggat tcgcggccga
     3181 tgtccgtggt gaagaagtcg cccggctccg ggatctgatg gaccaggccg ccgatcagcc
     3241 agacgcgcgt ccacacgtgc tcccactcga gctcggcgaa ctccctggac cggtaacggt
     3301 cgccgttgat cgggttgctc ccaagctcgg gctccgtggc cttggtccga tcaattcctc
     3361 gcatctgcct tatgctgctc atggcatgct cctgtctggt tgaccgcacg gcgggtgcgc
     3421 acggacggct tattctgccc gcttgggggt ggcattcaag cccggtgcgc gggccggcga
     3481 acgctcagct ccagatctcg aaaaactcct cgaacgcctt gagctggttg cccacggcgg
     3541 acgagggcac gatgatgggc gtgcggacgc cggcgtcgaa ccaggcctcg atgccgtcgc
     3601 ggaccgcgtt cgcgggaccg aacaaagttg tctcggcgag ccacgcatcg gacaggcaat
     3661 cggggatcct gtcgggctcc cctgcctcga gcgccttctc cacggcaccc atctcctcca
     3721 cgaagccggc ttctttccag tagttgcggt agttcgggag catggcgtaa cccgtcaggg
     3781 tccggcggtt gacggctttt gccgcctcca cgtcgtcgct gatgcaggtg gggatcatgt
     3841 tgccgatgaa gaaaccgggg tcggcgcgcc gctcgtcacc gagcacgttc agcgagtgct
     3901 gcatatggcc gcgcgcggcg ttggcgaaca ccatgccctc ggcgacctcc gcggcaagcc
     3961 cgatcatcct gtcgcgcagc gtggccagca ccacgggcgg cagctcacct acccgcggcg
     4021 cggcgcgcag gtcgctcacg aactggcgca tgtccgacag gggcttgccc tgccggacgc
     4081 ccagacgctc catggccggt gcgtggctga cccccagccc gaagcggaac cggccattgg
     4141 agatctcgtg aatgaatgcc gcggtggaag cgaagtcgtt cgcgtggcgg gtgtagatcg
     4201 gcgtgatgct ggtcccgaac tcgatctgtc gggtgttgta ggcaatggcc tcgcacagcg
     4261 ccaggccgtc tcccatgctc gggcagtaga tgcccgcaaa accgcggcgc tcgatctctg
     4321 cggcgagctc gacgatggtg ttacggcggc ccgggaccgc ggcgaggctt actgcgggtc
     4381 gtcgatcgct catgctgagt tctccctggg aagtatgcgg aaagcgacac agagtggcgg
     4441 aacctcgtgc gcttcgcaac cgcgtttgcc gccatgtttt cggccgcggc ggccgcgcgt
     4501 ctcagaacca gcggtgtcgc gaggagcgcc tctcgggctg ggcgagatgc tgtgcgaggt
     4561 ggttcggccg gcggaacagg tgtatcagag ccaccccggc cagaaacccg ccgatgtgag
     4621 cccagaaggc gacgcccccc tggctcgagc ccattgccgg caaaccctga agcagctgca
     4681 ggagaaacca gtagcccagc atgaaaaccg cgggaacggc cacggtggtc acgtagaagc
     4741 cgagaaagat cagcgtgtgc acgtgggcgc gtgggaacat gagggcgtag gcacccatga
     4801 cgccgccgat ggcgcccgat gcgccgacca tcggcaccag gctcgacgtg ttcgtggcga
     4861 tctgggcgaa ggcggcggcc aggccgcaga cgacgtagaa gacgacgaac cggaacggtc
     4921 ccatgacgtc ctccacgttg tctccgaaaa cccacagaaa ccacatgttc ccgatgatgt
     4981 gaaaccagcc gccatgcatg aacatggacg tgaagaggga ggcaggattg gcggtcccgt
     5041 caagctggca gacgaggttg ctgcccagcg gaatgctcgt gccgggaacg gcgttgccca
     5101 gcaggtcgcc cggtatcagc ccgtacagac acagggactc ggccagcgcg tagtggttgc
     5161 caaatccctg gacggcgacc cagacgagga cgtttagaat gatgatggca acggtggcgt
     5221 acggcgggtg aacccgggga ttttcgtcgc gcagtggaaa catgatcggc tcgtatggac
     5281 ctcaggcacg gtgttggctc ggcgcgtcgg cagatgggcg catgaaaatc tagggtgccg
     5341 ggatgtcggc gccgccgcaa gaccgggcat ggtcttgaag gctcagaacg tcaggctgat
     5401 cttgtccgcc gtgggcaggt cgcccggctc gagcgcctcg cgcttggcgg ccttgaacgc
     5461 ttcgatatcg ccaacgacca tctgccagac cgggtgcatg tcccgcccgt ccaccggcat
     5521 gggaaagggg ctgaagccaa ggggcacgct ggcgaacggc tcgtcttggc gtcggtagag
     5581 agtctgctcg gaaaaatcca gatacctggg cagaccatcg cgcgagacca tgtcgaagaa
     5641 agcgacggca ccccgaccgg ccaggtccag ac
//
LOCUS       DBNHOX010000238         6431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_84246, whole genome shotgun sequence.
ACCESSION   DBNHOX010000238 DBNHOX010000000
VERSION     DBNHOX010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_84246"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="AC16UZ_05280"
     CDS             complement(<1..794)
                     /locus_tag="AC16UZ_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR6246129.1"
                     /translation="MIKWLSSRKRQVLAGFTAMALLLSGYTFADELEDRLREAREQLD
                     AAAERLAELHRQMYEAEVSEGRYGKRAMIGILLTSMGDDDGLVIAGVTPDGGAKQAGL
                     AAGDRIVAIGGVRLDTGGADKPGKLLSNVVKDLEPGDPVKVEYVRDGETLKADVVTQP
                     KRAFVMKSLAMELDQLDPEFTAELIKEFEDLGELKELKELGKLEALHELGDLGELIGN
                     VVDVGTSLKLEDMDGDLARYFGVDAGVLVVAPPSQEGELKSGDVLL"
     gene            complement(804..1421)
                     /locus_tag="AC16UZ_05285"
     CDS             complement(804..1421)
                     /locus_tag="AC16UZ_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246130.1"
                     /translation="MESKTPLADLLAYRDGEELPADVARGIEGDPAAQGELEKLRGIR
                     QQLNALPAVLPDPAVWEEISRRAQPPRSWTLRYPLATAATVFLAAALTIVAWNPLGNG
                     GAGAGPEPVVVADGALVDLMSRSQALESHLPVQGTPAGQLAWGSAEEALRYRIADIDA
                     QLMRIYETEPVDVERREALWRQRVDLLESLTQVQRGQAVVRPAIY"
     gene            complement(1378..1974)
                     /locus_tag="AC16UZ_05290"
     CDS             complement(1378..1974)
                     /locus_tag="AC16UZ_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6246131.1"
                     /translation="MASFAEIRLEPGVARGLKRGDRSAQGIAYRVLAPTVMGLACRIL
                     QDEGSAQEVVQDTFVDLIEHARTLKDAQAVVGWVRQVAVNHCLMRLRSPWNKRRDELD
                     ADPAGDVYVDMAEGAARTEGMQDIEKALSELEPETRLVIWLHDVEGYTHQEIAGLVGR
                     TASYSKSQMARGYQKLAKRFRGTEDGIENTASRSARVP"
     gene            complement(2033..3310)
                     /locus_tag="AC16UZ_05295"
     CDS             complement(2033..3310)
                     /locus_tag="AC16UZ_05295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6246132.1"
                     /translation="MKFGIFYEHQLPRPWDDGAEQRLFNEALDQVELADRLGIDYAWE
                     VEHHFLEEYSHSSAPEVFLAACSQRTKNIRLGQGIRQVIPQYNHPARTAEVIATLDLV
                     SQGRVEFGTGESSAELELGGFRIPVAKKRKIFMEATEQICNMLAMDPYPGYKGQYFEM
                     PCRNVVPKPVQRPHPPVWVACSQRETIRMAARLGIGALTFAFVDPMEAQSWVDEYYGI
                     IKSDQCVPIGHAVNANICMVSSFSCHHDRQTAVQRGLEGFEFFGYALGSLYGFGEHKP
                     GRTDLWTEFQTVRAPQVRNEVADFSEALLGARGGIGTPEDLRQHLRKFERCGVDQVTF
                     IQQAGMNRHEHICESLEIFAAQVMGEFKEREAEREERKMRELEPYIAAAMERKVRMKP
                     LADDEIPTFPALGRRITEEQEDAPSIYERPAAG"
     gene            3457..4059
                     /locus_tag="AC16UZ_05300"
     CDS             3457..4059
                     /locus_tag="AC16UZ_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6246133.1"
                     /translation="MTDRTFCYPHFVGNNDKPAANSRAELENRLARVFIEHGYEGASL
                     SRLATASGLGKASLYHHFPGGKAEMASVLLTRAIARLDKTAFSRLTKKQPAPVRLGRF
                     VDAFSSYVDHGERPCLIAVLDHGALRAVHGDTIRRRFLSWGERLAATFEETGMKPRRA
                     RRAASDLLAGLYGNLAMARMLDDASLFQRGTRRLKKDLPS"
     gene            complement(4056..5495)
                     /locus_tag="AC16UZ_05305"
     CDS             complement(4056..5495)
                     /locus_tag="AC16UZ_05305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase family protein"
                     /protein_id="HIR6246134.1"
                     /translation="MAKYDNNLIVIGAGSAGLIVALIAATVRARVTLIEQGAMGGDCL
                     NTGCVPSKTLIRSAKIAHYQATAQRFGVQAVTPQVDFQSVMRRVHEVIATIEPKDSMA
                     RYRELGVNCVAGAARLIDRHHVQVGDERLSARSIVLATGAEPVVPPIPGLADARPLTS
                     DNLWELESLPQRLLVMGGGPIGCELAQAFARLGSAVTLIDMAPGLMPREDDDVAGFVE
                     RRLRGEGIDVRTGHRAVRVVASADGAGVLHADSDGGEVQIGFDRVLVAVGRRANTAGL
                     GLEALGIATRSDGTLEVDAYLRTAVRNVYGCGDVVGPYQFTHMASHQAWYAAVNALFG
                     RFRKFKVDYSVVPWVTFTDPEVAHVGLSETEAAERGIRVEVTRYGLDDLDRALADGSA
                     SGWVKILTRPGSDRILGVSIVGAHAGELIAEYVLAMTHGIGLKKILGTIHVYPTLAEA
                     NKLAAGAWRRAHAPERLLRWVGRLHDLLR"
     gene            complement(5524..6189)
                     /locus_tag="AC16UZ_05310"
     CDS             complement(5524..6189)
                     /locus_tag="AC16UZ_05310"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR6246135.1"
                     /translation="MLVLVAGLLGAFFLTGLNDYLSLSFLKGVHHDAVVFVRENALVS
                     ILGFFLFYIAVTALSLPGAAIMTLAAGAVFGLFWGTLITSFASSAGATLAMLISRTLL
                     RDWVHARFAAQLRVVNDGLARDGAFYLFSVRMVPLFPFFIVNLVMGLTAIGTWQFYWV
                     SQVGMLAGTLVFVFAGTQLANVDTVADVLSPGLIAALSLLGLFPLIARKTMGWLERAR
                     AAR"
BASE COUNT         1119 a         2194 c         2047 g         1071 t
ORIGIN      
        1 tcgagcagca cgtccccact cttgagctcg ccttcctgtg aaggcggagc gaccacgagg
       61 acgcctgcgt cgactccgaa atacctggcc aggtcgccgt ccatgtcttc gagcttcaag
      121 gaggtgccca cgtcgacgac gttgccgatc agctcgccca gatctccgag ctcgtgaaga
      181 gcctccagct tgccgagttc tttgagttcc ttgagctctc cgaggtcctc gaactctttg
      241 atgagctcgg ccgtgaactc gggatcgagc tgatccagct ccatcgccag ggacttcatc
      301 acgaaggcac gtttgggctg cgtgaccacg tcggctttca gcgtctcgcc gtcgcgcacg
      361 tattccacct tcacggggtc gccgggctcg aggtccttga ccacgttgct gaggagcttg
      421 ccgggtttgt ccgcaccgcc ggtgtccagc cgcacgccgc cgatggcgac gatccgatcg
      481 cccgcggcca gtccagcctg cttcgcgccg ccatcgggcg tcacgccagc aatcaccagg
      541 ccgtcgtcgt cgcccatgct cgtcagaagg ataccgatca tcgcgcgttt cccgtagcga
      601 ccctctgaaa cctcggcttc atacatctgc cgatgcagct cggcgagccg ctcggcggcg
      661 gcatcgagct gttcgcgggc ctcgcgcagc cggtcctcca gctcgtcggc aaaggtatat
      721 ccggagagca gcagtgccat cgccgtgaac ccggcgagca cctgccgctt ccgcgagctc
      781 aaccacttga tcatcatcga ctcctagtaa attgcaggtc gcaccacggc ctggccgcgc
      841 tgcacctgag taagcgattc gagcaggtcg accctctgcc gccacagcgc ttcacgacgc
      901 tcgacgtcca ccggttcggt ctcgtagatc cgcatgagct gcgcatctat atcggcaatc
      961 cggtacctga gcgcctcctc ggcagagccc caggccagct gaccggcggg cgtgccctgc
     1021 acgggcaggt gcgactcgag agcctgggat cggctcatca ggtcaaccag ggcaccatcg
     1081 gccaccacga cgggctctgg accggcgccg gctccgccgt tgccaagggg gttccaggcg
     1141 acgatggtga gggccgccgc cagaaagacc gttgccgcgg tcgccagggg gtagcgcagc
     1201 gtccagctgc gcggcggttg cgccctgcgg ctgatctcct cccacaccgc cggatcggga
     1261 agaacggctg ggagcgcgtt gagctgctgc cttatgccac gcagcttctc cagctcgccc
     1321 tgggcggcag ggtcgccttc gatgccgcgc gccacgtcgg cgggcaactc ctcgccgtca
     1381 cggtacgcga gcagatcggc tagcggtgtt ttcgattcca tcttccgttc ctctgaatct
     1441 cttcgcgagc ttctggtagc ctcgcgccat ctgggacttc gagtagctgg cggtgcggcc
     1501 caccagccct gcgatctcct ggtgggtgta gccctccaca tcgtggagcc agatcaccag
     1561 ccgggtctcc ggctccagct cgctcagcgc tttctctatg tcctgcatgc cctccgttcg
     1621 ggccgcccct tcggccatgt cgacgtatac gtcccctgcc gggtccgcat cgagctcgtc
     1681 ccgccgcttg ttccaggggg aacgcagccg catcaggcag tggtttacgg caacctgccg
     1741 cacccagccg acgaccgcct gcgcgtcctt gagcgtgcgc gcgtgctcga tcagatccac
     1801 gaaggtgtcc tgcacgactt cctgagccga tccctcgtcc tggagaatgc ggcaggcgag
     1861 gcccatcaca gtgggcgcga gcacacggta ggcgatgccc tgggcactgc ggtcaccccg
     1921 cttgaggccc cgggcaacgc ctggctccag gcgaatttcg gcaaaactgg ccataccact
     1981 ctatagatgc gtcagacccg caaagggtcg cagcaacgga gatttttttc ggctacccgg
     2041 ccgccgggcg ctcgtagatg gacggggcgt cctcctgttc ctcggtgatc cgccgtccga
     2101 gcgccgggaa agtcggtatc tcgtcgtccg cgagcggctt catcctcacc ttgcgctcca
     2161 tggcggcagc gatgtaaggc tcgagctcac gcatcttgcg ctcctcccgc tccgcttccc
     2221 gctccttgaa ctctcccatg acctgcgcag cgaagatctc gagagactcg cagatgtgct
     2281 cgtgccggtt cattccggcc tgctggatga aggtcacctg atccacgccg cagcgctcga
     2341 acttgcgcag atgctggcgc agatcctcag gggtaccgat accgcctctg gcgcccagca
     2401 gagcctcgct gaagtcggcc acctcgttcc tgacctgagg cgcgcgcacg gtctggaact
     2461 ccgtccagag atccgtgcgg cccggtttgt gctcgccaaa gccgtagagg ctgccaaggg
     2521 cgtagccgaa gaactcgaag ccttccaggc cgcgctgcac ggcggtttgg cggtcgtggt
     2581 gacaggagaa gctggacacc atgcagatgt tggcattcac ggcgtgaccg atgggcacgc
     2641 actgatcgct cttgatgatg ccgtagtact cgtcgaccca ggactgagcc tccatggggt
     2701 cgacgaaggc gaaggtcagc gccccgatgc ccagccgggc ggccatccgg atggtctcgc
     2761 gctgagagca ggccacccag acgggcggat ggggccgctg cacgggcttg ggaaccacgt
     2821 tccggcaggg catctcgaaa tactggccct tgtacccggg atacgggtcc atggcgagca
     2881 tgttgcagat ctgctcggtg gcctccatga agatcttgcg cttcttggcc acgggaatcc
     2941 ggaagccgcc gagctcgagc tccgccgagg actcgcccgt gccgaactcc acgcggccct
     3001 gggagacgag atccagggtg gcgatcacct cggccgtgcg ggccggatga ttgtattgcg
     3061 ggatcacctg ccgaatgccc tgacccaggc ggatgttctt ggtgcgctgg gaacacgccg
     3121 ccaggaagac ctcgggcgcc gaggagtggg aatactcctc gagaaagtgg tgctcgacct
     3181 cccaggcata gtcgatgccc agcctgtccg cgagttccac ctggtccaac gcctcgttga
     3241 acagccgctg ctccgcgccg tcgtcccagg gccgcggcag ctgatgctcg tagaagatcc
     3301 caaatttcat gttcatgtcc ccactgtgtt gtgcagatcc ccggcggccg ggcggccagg
     3361 gccccgaatg gcgctttccc gctgcccgag tctcccctca aagggcacgt cgcatcaagg
     3421 ggcaccgcgc cagcggcgcg gatcagacgg cgccttttga ccgaccgaac gttctgttac
     3481 cctcatttcg tgggcaacaa cgacaagcca gctgcgaata gtcgcgccga gctcgaaaat
     3541 cggctcgcca gagtcttcat cgagcacggc tacgagggcg ccagcttgag ccgcctggcg
     3601 accgccagcg ggctggggaa agccagcctc taccatcact ttcccggcgg caaggcggag
     3661 atggcgtcgg tgctcctgac cagggcgatc gcccggctgg acaaaacggc cttttcccgg
     3721 ctgaccaaga agcagcctgc cccggttcgt ctcggccggt tcgtggacgc tttcagcagc
     3781 tacgtggatc acggcgagcg cccctgtctg atcgcagtgc tggaccacgg cgcgttgcgg
     3841 gccgtgcacg gtgacaccat ccgccggcgg tttctgagct ggggcgaacg gcttgccgcc
     3901 acgttcgagg aaaccggcat gaaaccgcgc cgggcgcgtc gggcggcttc cgatctgctg
     3961 gccggactgt acgggaacct ggcaatggcc aggatgctgg acgatgcgtc gctgttccag
     4021 cgcggcacca ggcgactgaa gaaggacctt ccttcctagc gcaacaggtc gtggagcctg
     4081 ccgacccagc gtagcagccg ttcgggcgcg tgggcgcgcc gccatgcccc cgcagcgagc
     4141 ttgttcgcct cggcgagggt cgggtagacg tgaatggtgc cgagaatctt cttcagcccg
     4201 atgccatggg tcatggccag cacgtactcg gcgatgagct cgcccgcatg ggctcccacg
     4261 atggaaacgc ccagaatgcg atccgagccg ggtctggtaa ggatcttcac ccaacccgag
     4321 gcgctgccgt cggcgagcgc ccgatccaga tcgtccagac cgtaccgagt gacctcgacc
     4381 cggatgcctc gctcggcggc ctcggtttcc gagagcccga cgtgggcgac ttccggatcc
     4441 gtgaacgtca cccagggcac caccgagtag tcgaccttga acttgcggaa ccggccgaac
     4501 agcgcgttga ccgccgcgta ccaggcctga tgagaggcca tgtgagtgaa ctggtaggga
     4561 cccaccacgt cgccgcagcc gtagacgtta cgcaccgccg tgcggagata ggcgtccacc
     4621 tccagcgttc cgtctgaccg ggtggcgatc cccagcgctt cgagccccag ccccgccgtg
     4681 ttggcgcggc gcccgacggc aacgagcacc cgatcgaagc cgatctgcac ctcgccgccg
     4741 tccgaatcgg catgtagcac cccggcgcca tccgccgagg ccaccacccg caccgcgcgg
     4801 tggccggtgc ggacgtcgat gccctcaccg cgcagcctgc gctcgacgaa gccggcgacg
     4861 tcgtcgtcct cccggggcat caggccgggc gccatgtcga tgagcgtgac cgccgagccc
     4921 agtcgggcga aagcctgagc cagctcgcag ccgatcggcc ctccacccat caccagaagc
     4981 cgctggggca ggctctcgag ctcccagaga ttatcggagg tgagcggccg ggcatcggca
     5041 agcccgggaa tgggcgggac aacgggctca gccccggtgg cgagaacgat gctgcgggcg
     5101 ctcaggcgct catcgccaac ctgcacgtga tgccggtcga tcaggcgcgc cgctccggcc
     5161 acgcagttga cgccgagttc ccggtagcgg gccatggagt ccttgggttc gatggtcgcg
     5221 atgacctcgt gtacccggcg catgaccgac tgaaagtcca cctggggagt caccgcctgc
     5281 accccgaagc gctgggcggt ggcctgataa tgagcaatct tggccgagcg gatcagcgtc
     5341 ttgctcggaa cgcaccccgt gttgaggcag tcgccgccca tggcgccctg ctcgataagg
     5401 gtgacccggg cccggaccgt ggccgcgatc agggcgacga tgaggccggc cgagcctgcg
     5461 ccgatcacga tcaggttgtt gtcgtacttc gccatgccct gtgaccccca cggaaactgc
     5521 agctcatcgc gccgcccggg cgcgctcgag ccatcccatg gtcttgcgcg cgatcagcgg
     5581 aaacaacccg agcaaactga gggctgcgat gagccccggg ctgagcacgt cggcaacggt
     5641 gtccacgttt gccagctgag tgcccgcaaa tacgaagacg agcgtgccgg ccagcattcc
     5701 cacctggctg acccagtaga actgccaggt accgatggcg gtgagcccca tgaccagatt
     5761 gacgatgaag aacggaaaca gcggcaccat gcgcacgctg aaaaggtaga acgcgccgtc
     5821 gcgtgccagg ccatcgttga ccacccgaag ctgggcggca aagcgcgcgt gcacccagtc
     5881 gcgcaggagc gtgcgggaga tgagcatggc cagggttgcg cccgcgctgc tggcaaagga
     5941 cgtgataagg gtgccccaga acaggccgaa cacggcccct gcagccagcg tcatgatcgc
     6001 agcgccaggc agcgagagcg ccgtgaccgc gatgtagaag agaaagaagc caaggatgct
     6061 caccagcgcg ttttctctca cgaaaacgac ggcatcgtgg tgaacgccct tgagaaagga
     6121 gaggctgaga tagtcgttca ggccggtcag gaaaaaggcg ccgagaagac ctgcgacgag
     6181 cacaagcacc aagatctttc gatggcggcc gcgggattcc gcccccgggg cgccggaacc
     6241 ggatccgctc atacgctgga gtccgcgtcg cgttgcatta ccatagccgc ccaggcacag
     6301 agaaagccag catcgcaaga gttaggacac gcatcatgcc gcgcagtttc cccgcgacgc
     6361 gcctgcgacg caatcgacag accgacttca tccggcgcct cgtgcgcgag accgagctga
     6421 cggtcaacga c
//
LOCUS       DBNHOX010000239         1811 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_276922, whole genome shotgun sequence.
ACCESSION   DBNHOX010000239 DBNHOX010000000
VERSION     DBNHOX010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_276922"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..668)
                     /locus_tag="AC16UZ_05315"
     CDS             complement(9..668)
                     /locus_tag="AC16UZ_05315"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6246136.1"
                     /translation="MECDERLLELISENLARFEASDADVDDAHHAAVAITVTDVGPGA
                     DLPGLSGTDGWQSQAGLLLTRRSAQLRKHAGQWAFPGGRLDPGETPAETALREMHEEV
                     RVDLGPDRILGRLDDFVTRSGFVMTPFVVWGGAGLQASPNPDEVQSIHRIPLTEFLRE
                     DAPVLEDADSSDRPVLKMPVGTEHIAAPTAALIYQFREVCLLGRHTRVAHYEQPAFAW
                     R"
     gene            777..1661
                     /locus_tag="AC16UZ_05320"
     CDS             777..1661
                     /locus_tag="AC16UZ_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6246137.1"
                     /translation="MSHDSERQALVAALAAVLLWSTVATGFKLGLAVMAPLQLLLAGT
                     TVSAIFFAAVTTARRSWSVTGRQLGEAAAFGIVNPALYYLVLFEAYDRLPAQLAQPLN
                     YTWAITLAVLAVPILKQRLSARTLAGILVSYLGVLVLLSQGRFDRLPAVDWTGVMLAL
                     GSTLLWAGYWLMNARSRSDSVNLMALSFLIATPLVAIACVLGPGLPTLDWNVAWFGAW
                     VGLVEMGVTFLLWQRAMGLTSHAARIGQLIFLSPFISLMLISTVLGETSHWTSVAGLV
                     VIVGGVLLTRSAAPGRAR"
BASE COUNT          250 a          643 c          594 g          324 t
ORIGIN      
        1 cgggcggatc agcgccaggc aaaggccggc tgctcgtagt gggcgacccg agtgtgccgg
       61 ccgagcaggc agacctcccg gaactggtag atcagcgccg cggtgggcgc tgcgatgtgc
      121 tcggttccca ccggcatctt cagcacggga cggtcgctgc tgtcggcatc ctccagcacc
      181 ggcgcgtcct cccgcagaaa ctcggtgagc ggtatgcggt ggatggactg cacctcgtca
      241 ggattcggcg atgcctgaag acccgccccg ccccagacga cgaagggcgt catcacgaac
      301 ccggagcgcg tcacgaagtc gtccagacga ccgaggatcc ggtcggggcc aagatcgacc
      361 cgcacctcct cgtgcatctc ccgcagcgcg gtctccgccg gcgtctcgcc ggggtcgagc
      421 cgcccgccgg gaaacgccca ctggccggca tgcttcctga gttgcgccga gcggcgggtg
      481 agcagcaggc ctgcctggga ttgccatcca tcggtgcctg aaagacccgg cagatcggca
      541 ccggggccga cgtcggtgac ggtaatggcc accgcggcgt ggtgggcgtc gtccacgtcc
      601 gcatcgctgg cttcgaagcg ggccaggttc tcgctgatca gttccagcag gcgctcatcg
      661 cattccatcg aagcagccgt caatttgggc ggtgtgtggc gagccccctt cggggcccgt
      721 tcgcgaatca tgcgccctgg tgtacgctga cgcaatttca cgccccggcg accccattga
      781 gccacgactc ggaaaggcag gcactcgtcg ccgcgctcgc ggccgtgctg ctgtggtcca
      841 cggtcgccac cggcttcaag ctggggcttg cggtgatggc gccgctgcag ctgctgctgg
      901 ccggaaccac ggtctccgcc atcttcttcg ccgccgtcac caccgcgcgc cgcagctggt
      961 cggtgaccgg ccgccagctg ggggaagcgg ccgcgttcgg catcgtcaat cccgcccttt
     1021 actacctggt tctgttcgag gcctacgacc ggctgccggc ccaactggcc caacccctga
     1081 actacacctg ggccatcacc ctggcggtgc tggccgtccc catcctgaag cagcgtctct
     1141 cggcgcgcac cctggccggt atcctcgtga gctatctggg ggtgctcgtc ctgctctccc
     1201 aagggcgctt cgaccgcctg cccgccgtgg actggaccgg cgtgatgctg gccctgggca
     1261 gcacgctgct gtgggccggc tactggctga tgaacgcccg ttcccggagc gattccgtta
     1321 acctgatggc tttgagcttt ctgatcgcga ctcccctggt agccatcgcc tgcgtgctgg
     1381 gccccggcct gccgaccctg gactggaacg tcgcgtggtt cggcgcgtgg gtgggtctgg
     1441 tggagatggg ggtgactttt cttctgtggc agcgggccat ggggctgacc tcccacgcgg
     1501 ctcgcatcgg ccagctcatc tttctctcgc ccttcatttc cctgatgctc atctcgacag
     1561 tgctgggtga aaccagccac tggacgtccg tcgccggcct cgtcgtcatc gtcggcggcg
     1621 tgctcctgac ccgatctgcg gcgcccgggc gggctaggtg attcccgcgg tatcgcgacg
     1681 ccgccaagtc gggcaccatc ggcccactgt cacaagtgga tgcaacccgt gatcaggacg
     1741 actaccgcgg tggtgaccgc cctcctcgca ctggccgccg ccccgaacct cacggcctcg
     1801 gagtcggaca c
//
LOCUS       DBNHOX010000240         4684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1305447, whole genome shotgun sequence.
ACCESSION   DBNHOX010000240 DBNHOX010000000
VERSION     DBNHOX010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1305447"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="AC16UZ_05325"
     CDS             <1..491
                     /locus_tag="AC16UZ_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-containing monooxygenase EthA"
                     /protein_id="HIR6246138.1"
                     /translation="NLEVMSGVEFWVDEAKVNFPDTFTYKGMMFSGVPNLVQTFGYIN
                     ASWTLRADLTSEYVCRLVNRMDELGVSQVAAQLREQDRDMPAKQWIDDFTPGYMQRSM
                     HLFPKQGEKDPWRNTQNYAQDKKAIRHAPLEDGVLTFCNPDSAAPRLAGRASVARDQD
                     AA"
     gene            510..1364
                     /gene="rfaP"
                     /locus_tag="AC16UZ_05330"
     CDS             510..1364
                     /gene="rfaP"
                     /locus_tag="AC16UZ_05330"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011703.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HIR6246139.1"
                     /translation="MSGRERIELRSDLGQRWHGLDVFDVVDDLRGESVREMAGRSTIR
                     FELDGAGFYAKRHFGTGWREIFKNLLTLRRPVIDAGNEYRATRALSAAGVSTLEIAGF
                     GLRGRNPARRRSFLITDELAPVKSLEAYVLRWPEEPPAPGLKRRLIEEVAAISRAMHG
                     AGINHRDYYLCHLLMLHPELFGPQAPDPRIFVVDLHRAEIRNAVPYRWLVKDLGSLYF
                     SAYEIALTRGDRLRFLAAYFQMPWRDVLARHSGLIADIAKRADRLYRKGRRKGILPRQ
                     VAGLAPTR"
     gene            complement(1372..1447)
                     /locus_tag="AC16UZ_05335"
     tRNA            complement(1372..1447)
                     /locus_tag="AC16UZ_05335"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1412..1414),aa:Lys,seq:ttt)
     gene            complement(1548..1961)
                     /locus_tag="AC16UZ_05340"
     CDS             complement(1548..1961)
                     /locus_tag="AC16UZ_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246140.1"
                     /translation="MIPNPSKALVDLAMKLSTGVLPEIGSPYAAANAGMTAVLLIMLS
                     QDSERAIANRMTDIDELKALFADAAAFPDRVGLASFVDAEPDSLLLADVDRLHADGMR
                     LLIDLHAWAESHDAGLDQEIWAFLGRHADRNRFDV"
     gene            complement(1958..3001)
                     /locus_tag="AC16UZ_05345"
     CDS             complement(1958..3001)
                     /locus_tag="AC16UZ_05345"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6246141.1"
                     /translation="MSAVLDTLGERFAAYLTKSWKQPATTLHIEQIPGGASRETYRVR
                     VSIGGQEQGLILRRDPVSSLIDTERSLEYRTYEAVFPTSIPVPEPLLLEEDAVHLERP
                     FSVMREIGGCESSAQSLAAPPYQAMRETIGRRKWEILGQLASLDLSELGVDAFMAVPD
                     DPARRELEHWSKVIADDALHPQPIAEAARRWLERNLPEPSGRFALVHGDYRSGNFLYD
                     EEGVIRGVLDWEMAHIGDPLEDLAWSLDPLWSWPERELAGSLLPREQAIGIWEQASGI
                     TVDRDAFRWWQVFASYKGLAIWISSTEDFVNGASKEPILAVAGWLMSDRQNRILVDRL
                     NPRSDHAYAEPIV"
     gene            complement(3021..4361)
                     /locus_tag="AC16UZ_05350"
     CDS             complement(3021..4361)
                     /locus_tag="AC16UZ_05350"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6246142.1"
                     /translation="MAEAYEFHDDDVIAPEVYGRHGHPHGAFAWLRANDPLRYVEPTE
                     YLPFWAVTKHADIVEIEKQPEIFASEPRPILVKEAQSPEVRQDVLMQIFERLKDSPKL
                     LEVLATIEQGGLIRSLVQMDPPDHPKYRTLVQPWFKPSNIKRLEDSLTRIIANILDEM
                     MGDGSERELEFVQDVAVWPPLKLITELLGVPEEDEWRILKLTNELFAGDDPEMKRAGD
                     DPLSIIDTIKDLYDYFTRLTDERRANPTEDLSSYIANGRIDGEYLPYKELISYYIIVA
                     TAGHETTRTAISGGLLALLENPEQMSLLRNNIDDEDLIKLAVEEMIRWTTPVSQFSRT
                     AMRDYELRGKQIRQGDALGLFYASANRDEEIFDDPFNFRVDRQPNRHLAFGTGPHICL
                     GILLARLEMRIFFKQFLPRIEKMELVDRGPEYLKASFVHGLKHLPIRFQLRPAR"
     gene            4506..>4684
                     /locus_tag="AC16UZ_05355"
     CDS             4506..>4684
                     /locus_tag="AC16UZ_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246143.1"
                     /translation="MKLSKLSRSVEGKVVIVTGAASGMGRATARVFADEGAKVAAMDI
                     NAEPLNEVVDEIAAAG"
BASE COUNT          816 a         1428 c         1567 g          873 t
ORIGIN      
        1 tgaatctcga ggtgatgtcg ggcgtcgagt tctgggttga cgaggccaaa gtcaacttcc
       61 cggacacgtt cacctacaag ggcatgatgt tctccggcgt gccgaacctg gttcagacgt
      121 tcggctacat caacgcctcc tggacgttgc gcgctgacct tacctcagaa tacgtgtgca
      181 ggcttgtcaa ccgcatggac gagctcggtg tttcccaggt cgctgcgcag ttgcgcgagc
      241 aggatcgtga catgccggcc aagcagtgga tcgacgactt cacgccggga tacatgcagc
      301 gctccatgca tctgtttccg aagcagggcg agaaagatcc ctggcgcaac acccagaatt
      361 atgcccagga caaaaaggcc atcaggcacg ctccactgga ggacggggtc cttactttct
      421 gcaatccgga ctcggccgcg ccgagactcg ccggaagggc gagcgtggct cgtgatcagg
      481 acgcggcctg accgcaccgc ccgatccgta tgagcgggcg cgaacggatt gagctcagat
      541 cggatctcgg ccagcgctgg catgggctgg acgtcttcga tgtcgtggac gacctgcgcg
      601 gtgagagcgt ccgggagatg gccggccgct ccaccatccg cttcgagctc gacggagccg
      661 ggttctacgc caagcggcat tttggaaccg gctggcggga aatattcaag aacctgctga
      721 cgctgcgtcg cccggtcata gacgccggga acgagtatcg tgccactcga gcgctctcgg
      781 ctgccggggt gagcaccctg gaaatcgccg gcttcgggct gcgtgggcgt aaccccgcga
      841 ggcgccggtc gtttctcatc accgacgagc ttgcaccggt aaagagcctt gaagcctacg
      901 tgctgcgctg gccggaggag ccgcccgcgc ctgggctgaa acgccggttg atcgaggagg
      961 tggccgccat ctcccgcgcc atgcacgggg cgggtatcaa tcacagggac tactacctgt
     1021 gtcaccttct gatgctgcat cccgagctct ttgggccgca ggccccggac ccccgcatct
     1081 tcgtggttga cctgcatcgc gccgagatcc ggaacgccgt gccgtaccgg tggctggtca
     1141 aggatctggg cagcctctat ttctccgctt acgagatcgc cctgacccgg ggggaccgcc
     1201 tgcgatttct ggcagcctac tttcagatgc cgtggcgcga cgttctcgct cggcattccg
     1261 gtctgatcgc cgacatcgca aagcgggccg atcgcttgta ccggaagggg cgaagaaagg
     1321 ggatcctgcc acgccaggtt gccgggctgg cgccgacgcg atgagtggag gtggtgggtc
     1381 gtctggggct cgaacccagg accaacgggt taaaagcccg gtgctctacc aactgagcta
     1441 acgaccctct tgccggatgg cggcaaaaaa gagcgcgaat tgtagtcagc gtaagggccc
     1501 atctcaaccc ttgccggtgg ggtcgcttcc atgggagcgg cgcccgttca gacgtcgaag
     1561 cggttccgat cggcgtggcg tcccaggaat gcccagatct cctgatcgag cccggcgtcg
     1621 tgggattccg cccaggcgtg caggtcgatg aggagcctca taccgtctgc gtgcagccgg
     1681 tccacgtctg ccagcaggag cgagtcgggc tcggcatcga cgaaggacgc gagcccgacc
     1741 cgatccggaa acgccgcggc gtccgcgaaa agagctttga gctcgtcgat gtcggtcatg
     1801 cggttcgcga tggcccgttc gctgtcctgt gagagcatga tcagcaggac ggccgtcatg
     1861 cccgcattcg ctgccgcgta aggtgagccg atttccggaa gcacgccggt ggaaagcttc
     1921 atggccaggt cgacgagagc cttcgagggg ttgggaatca tacgatgggc tccgcgtagg
     1981 cgtggtcgga gcgggggttc aagcggtcca ccagaatccg gttctgacgg tcgctcatca
     2041 gccagccggc cactgccagt atgggctcct tggacgcgcc gttgacgaag tcctcggtgg
     2101 aggagatcca gatcgccagc cccttgtagg aggcgaatac ctgccaccag cggaaggcgt
     2161 ctctgtccac ggtgatgccg ctggcttgtt cccaaatacc gatggcctgc tcccgcggca
     2221 gcagggagcc ggcaagctcg cgctcgggcc atgaccacag gggatcgagg gaccatgcca
     2281 gatcctccag gggatcgccg atgtgggcca tctcccagtc cagcacgccc cgaatgacgc
     2341 cttcttcatc gtagaggaag ttgcccgagc gatagtcgcc gtggaccagg gcaaagcggc
     2401 ccgacggctc gggcagattt cgctccagcc agcggcgggc agcttccgcg atgggctggg
     2461 gatgcagcgc gtcgtcggca atgaccttcg accagtgctc gagctcccgt cgtgcaggat
     2521 cgtccggcac cgccatgaag gcgtcgacgc ccagctcgct taagtcgagc gacgcgagct
     2581 ggccgagaat ttcccacttt cgccggccga tggtctcgcg catggcctgg tagggcggtg
     2641 ctgcaaggga ctgcgcgctg gactcgcagc cgccgatctc gcgcatcacg gaaaacggcc
     2701 gttccaggtg gacggcgtcc tcctcgagca gcagcggctc aggcacgggg atcgacgtcg
     2761 gaaatactgc ctcgtaggtt cggtactcga gggaacgctc agtgtcgatc aggctggaaa
     2821 cgggatcgcg ccgaagtatg agtccttgct cttgcccacc gattgaaacc ctgacgcggt
     2881 aggtttccct ggaagcgccg ccgggaatct gctcgatgtg cagcgtcgtt gccggctgct
     2941 tccagctctt tgtcaggtaa gcggcaaagc gctcgcccag ggtatcgaga accgcgctca
     3001 tgacccggtt gcccgtgtgc tcaccgcgcc ggtctcagct ggaaccgaat cggcaggtgc
     3061 ttgagcccgt gcacgaagct tgccttgagg tactccggac cccggtcgac cagttccatc
     3121 ttctcgatgc ggggcaggaa ctgcttgaag aatatccgca tctccagccg cgccagcagg
     3181 atgccgagac agatgtgagg cccggtcccg aaggcgaggt gacggttggg ctggcggtcg
     3241 accctgaaat tgaaggggtc gtcgaagatt tcctcgtccc ggttggcgga ggcgtagaac
     3301 aggcccagcg cgtcgccctg gcgaatctgc ttgccgcgca gctcgtagtc ccgcatggcg
     3361 gtgcgggaga actgactgac cggcgtcgtc cagcggatca tctcctccac agcgagcttg
     3421 atcaggtcct cgtcgtcgat gttgttccgc agcagactca tctgctccgg gttttcgagc
     3481 agggcgagca gaccaccgga aatggccgtg cgcgtggtct cgtggccggc cgtcgcgacg
     3541 atgatgtagt aggagatcag ctccttgtac ggcaggtact cgccgtcgat gcggccgttg
     3601 gcgatgtacg aggaaaggtc ctccgtgggg ttggcgcgcc gttcgtcagt caagcgggtg
     3661 aagtagtcgt acaggtcctt gatggtgtcg atgatggaga gcggatcgtc gcctgcccgt
     3721 ttcatctccg ggtcgtcgcc ggcaaacagc tcgttggtga gcttgaggat ccgccactcg
     3781 tcctcctcgg gcacgcccag cagctcggtg atcagcttga gcggcggcca gacggcaacg
     3841 tcctggacga actccagctc gcgctcggag ccgtcgccca tcatctcgtc gagtatgttt
     3901 gcgatgatgc gggtcaagct gtcctcgagg cgcttgatgt tggatggctt gaaccagggt
     3961 tggaccagcg tccggtactt cgggtggtct gggggatcca tctggaccag cgagcggatc
     4021 aacccgccct gctcgatggt ggccagcacc tccaggagct tcggcgagtc cttgagcctc
     4081 tcgaagatct gcatcaggac atcctggcgc acctccgggc tctgtgcctc cttgaccagg
     4141 atcggccttg gttcgcttgc gaagatctcc ggctgcttct cgatctcgac gatgtcggcg
     4201 tgcttggtca cggcccaaaa ggggaggtac tcggtgggct cgacgtagcg cagcgggtcg
     4261 ttggcgcgga gccaggcaaa cgcgccatgg ggatggccgt ggcgtccgta aacctccgga
     4321 gcgatcacgt cgtcgtcgtg gaattcgtag gcttctgcca tcttcgaggg aactcccgcc
     4381 ggtgggtgaa gtggtttacg ttagcgggat tccccggccg attgcaatgt gcgatggtgc
     4441 tccctagagt ggcacgggac cgcatcgaac gggcagaccg ctccggccgt cacgaggtac
     4501 acgatatgaa gctcagcaag ctcagccgat cggtggaagg caaggtggtc atcgtcaccg
     4561 gtgcggccag cggtatgggg cgcgccacgg cgcgggtctt tgccgacgag ggcgccaagg
     4621 tcgcggccat ggacatcaac gctgagccgc tcaacgaggt tgtcgacgag atcgctgccg
     4681 cggg
//
LOCUS       DBNHOX010000241         2930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_727037, whole genome shotgun sequence.
ACCESSION   DBNHOX010000241 DBNHOX010000000
VERSION     DBNHOX010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_727037"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="AC16UZ_05360"
     CDS             <1..469
                     /locus_tag="AC16UZ_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246144.1"
                     /translation="WMSEDRRTSKPADLDQIARASDRTGPKGRATSRLNLIWNVFVFQ
                     LKLAFDGIRDVLLVPASLIAALAGLLAGGDEPDRHFKRLLEFGRRTETWLNLFGHRQD
                     GETSDDLIKPIQERVFETARDNPWLSKTGGQISQQLDGVNARLARRSESDSPD"
     gene            complement(503..1114)
                     /locus_tag="AC16UZ_05365"
     CDS             complement(503..1114)
                     /locus_tag="AC16UZ_05365"
                     /inference="COORDINATES: protein motif:HMM:NF019410.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HIR6246145.1"
                     /translation="MGQQYDPRNVFTNVHHNATGELLHLIDGFYLNMEDGLFELAFGD
                     DAEREHRRCFELMREMRYRRGQLVQNFANRMEKNVEAWFSVMDPPSRNEDEFALLAHA
                     VAEKCTSHFNVLLKTLAERTSFAQEQDFAPAHLPISPYHISHCFVVSCRSLKFDARSV
                     EIVHDLFGRFVLDRMGGIYGQANRLLYEAGYLSTSEVSQVLNG"
     gene            complement(1219..1722)
                     /gene="gspM"
                     /locus_tag="AC16UZ_05370"
     CDS             complement(1219..1722)
                     /gene="gspM"
                     /locus_tag="AC16UZ_05370"
                     /inference="COORDINATES: protein motif:HMM:NF016493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HIR6246146.1"
                     /translation="MLENVNKAFARSSVGQWYSGREPNERVIIGWLVFAVVLTVLWAG
                     VWKPLSDWRAVEDNRYQNAHSLLEWMQANESRARSAAKGQQGNRAQRSLLPTVTTAAQ
                     AEGLVLSRLQPESNGAVSVVLQGQEFNAVIRWLHRLQETNAVSVQRISLDADGRPGYV
                     NAQLRLR"
     gene            complement(1722..>2930)
                     /gene="gspL"
                     /locus_tag="AC16UZ_05375"
     CDS             complement(1722..>2930)
                     /gene="gspL"
                     /locus_tag="AC16UZ_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HIR6246147.1"
                     /translation="ATRDDEGYHLEAEWLITEDDGSIRANGQTDLRGLAELVDPETDW
                     LQTADNLVVIVPGDYVLGVSCEVPGRSAGQIRRALPFVVEEFVATDIEAMHLAAGEIQ
                     RGEPVRCCLVDRSLLEDWLVCLSEIGLSPGHMTAEAELLPAAEGQVSVLLEARRALLK
                     TLDQSATVDRSNLMMALESLTADKLLLVNGDPAALDFELERLEGLTILTGDEDAEAGS
                     TLAYLANRWLADGDGVNLLQGAYLPKRPVNPVWARWQGVAALAAAWAGVGIIAMIVEG
                     LWAGAQADRLQAESEALYRDIYPQERRITNVRRQMQAKLGVRTGGDGTGFTEYLAELS
                     ASLGQGATVTGLNYNDARDELGADLIVRSYDDAESLQTAIKDRGLNAGITSAEQQEGG
                     VRVRLRVGGA"
BASE COUNT          533 a          974 c          900 g          523 t
ORIGIN      
        1 gtggatgagc gaggaccgtc gtacatccaa gccagcagac cttgaccaga ttgccagggc
       61 gtctgaccga accggcccca aaggacgcgc cacctcccgc ctcaacctca tctggaacgt
      121 cttcgtcttc cagctgaagc tcgccttcga cgggatccga gacgtcctgc tggttcccgc
      181 ctcactgatt gcggccttgg ccgggctcct cgccggcggc gacgagccgg accggcattt
      241 caagcgcctg ctcgaattcg gtcggcgaac ggaaacgtgg ctcaacctgt ttggtcatcg
      301 ccaggacggg gaaacgtccg acgatctcat caagccgatt caggagcggg ttttcgaaac
      361 cgcgcgagac aatccgtggc tcagcaaaac gggcggacag atcagtcagc agctcgacgg
      421 cgtgaacgcg cgattggctc gcaggtcgga aagcgacagc cccgattgag gcccgtagcc
      481 ccgcgcaatt gagcgcgagt catcagccgt tgagaacctg cgacacctcg ctggtagaaa
      541 gatagccggc ttcgtagagc aaacgattgg cctgcccgta aattccgccc atgcgatcca
      601 gcacgaagcg cccgaagaga tcgtggacga tctccacgga gcgggcgtcg aacttcaatg
      661 atcggcacga gaccacgaag cagtgggaaa tgtgatacgg gctgatgggc aggtgggcgg
      721 gagcgaagtc ctgctcctgc gcgaaggacg ttcgctcggc aagggtcttc aagagcacgt
      781 tgaagtggga cgtgcacttt tccgccacgg cgtgagccag gagcgcaaac tcatcttcgt
      841 tgcgagacgg cggatccatg acgctgaacc acgcctcgac gttcttctcc atgcggttgg
      901 caaagttctg cacgagctgt ccgcgccggt agcgcatctc cctcatgagc tcgaagcagc
      961 gcctgtgctc ccgttcggcg tcgtcaccga aggcgagctc gaaaagcccg tcctccatat
     1021 tgagatagaa gccgtctatg aggtgtagaa gctcgcctgt ggcgttgtga tgaacattgg
     1081 tgaatacgtt gcggggatcg tactgctgac ccatcgcgcc ctccggttag tgtccctgca
     1141 aggaagtcta tgcaggcggg atcgggaaaa cgttgaccca ctcacccgcc ttcatgtgat
     1201 ttggctcgta attccgcctc aacgcaagcg cagctgcgcg ttcacgtagc cgggccggcc
     1261 atcggcgtcc agggatatcc gctgaacgct gacagcattg gtctcctgca gccggtgcag
     1321 ccacctgatc acggcgttga attcctgacc ctgcagcacg acgctcacgg caccgtttga
     1381 ctcgggctgc aggcggctca gcaccagacc ttcggcctgg gccgcggtgg tgaccgtcgg
     1441 cagcagcgag cgctgggccc gattgccctg ctgccccttg gcggcagacc gtgcccgcga
     1501 ctcgttcgcc tgcatccact cgagcaggga atgggcgttc tggtagcggt tgtcctccac
     1561 cgcccgccag tcgctcagcg gcttccagac gccggcccag agcaccgtga gcaccacggc
     1621 aaagacgagc cagcctatga tcacgcgctc gttgggctca cgtcccgagt accattgtcc
     1681 gaccgacgat cgggcgaagg ccttgttcac gttctccagc attacgctcc tcctactctc
     1741 aggcgcactc gcaccccacc ttcctgctgc tcggcggacg tgatgcccgc gttcagccca
     1801 cgatccttga tcgccgtctg aagcgactcc gcatcgtcgt agctcctcac gatgaggtcg
     1861 gcgcccagct cgtcccgggc atcgttgtag ttcagccctg taacggttgc gccctgcccc
     1921 aaagatgctg aaagctcggc cagatactcg gtgaatccag taccgtcgcc gcccgtgcgc
     1981 acgccgagct ttgcctgcat ctgccgtcgc acattggtga tccggcgctc ctgggggtag
     2041 atgtccctgt aaagggcctc gctttcggcc tgcagccggt cggcctgggc gccggcccac
     2101 aaaccttcga cgatcatggc gatgatgccc acgccggccc aggcggccgc gagcgcggcc
     2161 acgccctgcc agcgcgccca aacggggttg acgggccttt tcgggaggta agcgccctgc
     2221 agcagattga ccccgtctcc gtcggccagc cagcggttcg cgaggtaagc aagggttgaa
     2281 ccggcttccg cgtcctcatc gccggtcagg atggtgaggc cttcgagccg ttcgagctcg
     2341 aaatccagtg cggccggatc gccgttcacg agcagcagct tgtctgccgt caggctttcg
     2401 agcgccatca tcagattcga gcggtcgacg gtggcggatt gatcgagcgt cttcagcaat
     2461 gcccgccgag cctcgaggag cacgctcacc tgtccctcag ccgccggcag cagctcggct
     2521 tccgcggtca tgtgcccggg agagaggccg atctccgaaa ggcagacgag ccagtcctcg
     2581 agcagagaac ggtccaccag acagcagcgg acgggctcgc cgcgctggat ctcaccggca
     2641 gccagatgca tggcctcgat gtccgtggcg acgaactcct ccaccacgaa gggcagcgcc
     2701 cggcggatct gccccgcgct ccggccaggc acctcacagg aaacgccgag cacgtaatcg
     2761 ccggggacga tgacgacgag attgtccgcc gtctggagcc agtctgtctc cggatcgacg
     2821 agctccgcga gcccacgcag atcggtctga ccgttcgctc ggatagagcc gtcgtcctcg
     2881 gtaatcagcc actcggcctc gaggtggtag ccctcgtcat cgcgcgttgc
//
LOCUS       DBNHOX010000242         3068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_19159, whole genome shotgun sequence.
ACCESSION   DBNHOX010000242 DBNHOX010000000
VERSION     DBNHOX010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_19159"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="AC16UZ_05380"
     CDS             <1..625
                     /locus_tag="AC16UZ_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6246148.1"
                     /translation="MAAEMVALMAGLGHERFAVAGHDRGGRVAYRMALDHPEVVSRLV
                     ILDIIPTLDQFEALGWRGGLFGYHWYFLAQPPPLPETLIAHEPELYLRTAMSSWAGDF
                     SALDAAFPAYLAAFTPETIRASCDDYRAGAYVDTEIDRADREAGRRIGCPVLALWGEI
                     NGPRPGVLEAWQRWADDVQGVGLPCGHFLPEEAPEAVLAAMLEFLKA"
     gene            641..1720
                     /locus_tag="AC16UZ_05385"
     CDS             641..1720
                     /locus_tag="AC16UZ_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015648740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HIR6246149.1"
                     /translation="MHGLQLLVNRNDLEDTRWQQAPVPAPDELAPGEVLLRIDEFALT
                     ANNITYAVAGEMMRYWNFFPAPDGWGRVPVWGFATVVASNHAEVATGERLYGYLPMGS
                     HLLIEAGRVRANQLTDVSAHRVDLPPVYNQFQRVDAASEPAADAAQMLYRPLFMTSFV
                     LDHFLHANGFFGAQSVILTSASSKTALGLALLLARRGDVRVIGLTGRANVGFVEGLGC
                     YDEVVAYEDLATLDAAREVVSVDMAGNGEVLAALHNHFADNLKYSCLVGATHWDHRAG
                     AREMAGPEPELFFAPSHIQDTDWGPGGLEARFADAWDMLVERMSGLVTVVRADDPAEV
                     EAVYQRLLAGEVGPDVGYVLGVDVG"
     gene            complement(1766..>3068)
                     /locus_tag="AC16UZ_05390"
     CDS             complement(1766..>3068)
                     /locus_tag="AC16UZ_05390"
                     /inference="COORDINATES: protein motif:HMM:NF014165.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246150.1"
                     /translation="LNRARGEGFELDDWQSANVTNMEREFVEATAIPSDLVRASRLAQ
                     MKCEQAWREARARNDWETVKPLLAEVIGLARQQADALADAMHLSRYDALLEVYEPELR
                     IADIEPVFEDLKAFLPGALETALDRQGEVAPLEGPFTVEEQRQLSLEVMGLLGFDFDR
                     GRLDVSHHPFCGGVPDDTRITTRYSESSFIEALMATCHETGHALYQQGLPRDWRQQPV
                     GDSLGAAVHESQSLLMELQVCRSREFVGFLAPMIRRHFDRSETEPAWSTDNLYGHVTR
                     VARGYIRVDADEITYPLHVILRFELERQLLDGTLAVADLPEAWNDAMQRYLGLSTEGN
                     NADGCMQDVHWYAGLFGYFPTYTLGALTAAQWFATARKVMPDLASDIAAGNLEPLVAW
                     LRENIHGRGRQTTMQPLLEEVTGSTLDAGFYKRHIQARYLG"
BASE COUNT          474 a         1004 c         1049 g          541 t
ORIGIN      
        1 gatggcggcg gagatggtgg cgctgatggc gggccttggt cacgagcgct tcgccgtggc
       61 cgggcacgac cgcggcggtc gcgtggccta ccgcatggcg ctggatcatc cggaagtggt
      121 gagccggctt gtcatcctgg acatcattcc gaccctggat cagttcgagg ccctcgggtg
      181 gcgcggcggt cttttcggct accactggta cttcctcgct cagccaccgc ccctgccgga
      241 gaccctcatc gcccacgagc cggagctcta cctgcgcacg gccatgagca gctgggctgg
      301 cgacttctct gccctcgatg cggcctttcc tgcgtacctc gcggccttca ccccggagac
      361 gatccgcgcc agctgcgacg attaccgggc cggagcctac gtcgacaccg aaatcgatcg
      421 ggctgatcgt gaggctggcc gtcggatcgg ctgcccggtg ctggcactgt ggggcgagat
      481 caacgggccg cggcctggag tgctcgaggc ctggcagcgc tgggcggacg acgtccaagg
      541 cgtcgggctg ccctgcggcc acttcctgcc cgaggaggca cccgaggcgg ttctggccgc
      601 gatgctagaa tttctcaaag cctgaatagg gagaggatga atgcacggtc ttcagctgct
      661 ggttaatcgg aacgatctcg aggacacccg ctggcagcag gcgccggtcc cggctcccga
      721 cgagctggcc ccgggcgagg tgctgctgcg catcgacgag tttgccctca ccgccaacaa
      781 cattacctat gcggtggctg gggagatgat gcgctactgg aacttctttc cagcgcccga
      841 cggctggggc cgcgtgccgg tctggggatt tgcaaccgtg gtcgcgtcga atcacgctga
      901 ggtggctacg ggcgagcgcc tctacggcta cctgcccatg ggcagccatc tgctcatcga
      961 agccggccgg gtgcgggcca accagcttac cgacgtcagc gcccatcgcg tcgacctgcc
     1021 cccggtctac aaccagttcc agcgcgtcga tgctgcttcc gagccggccg ctgacgcggc
     1081 ccagatgctc tatcggccgt tgttcatgac ctctttcgtg ctggaccatt ttctccatgc
     1141 aaacggcttc ttcggcgcgc agtcggtgat tctgaccagc gcgtccagca agacggctct
     1201 cgggcttgcg ctgctccttg cgcggcgcgg ggacgttcgc gtgatcggtc tcaccggccg
     1261 ggccaacgtc gggttcgtgg aagggctcgg ctgctacgac gaggtagtgg cctatgaaga
     1321 cttggccact ctggatgctg cacgggaagt ggtgagcgtc gacatggccg gtaacggtga
     1381 ggtgctggcc gccctgcaca accacttcgc ggacaacttg aagtacagct gcctggtggg
     1441 ggccacccac tgggatcacc gcgctggcgc gagggagatg gcgggccccg aaccggagct
     1501 gttcttcgcg ccgagccaca ttcaggacac ggactggggc cccggcgggc tcgaagcccg
     1561 cttcgccgat gcctgggaca tgcttgtgga gcgtatgtcg gggctcgtca ccgtggttcg
     1621 ggccgatgac ccggcagagg tcgaggccgt ctaccagcgg ctgcttgctg gagaggtggg
     1681 accagacgtg ggctacgtgc tgggtgtcga cgtgggttga atcgcgccgc cccttttggg
     1741 gcgatccgat cgtggcgagg cggcctcagc cgagataacg cgcctgaatg tgccgtttgt
     1801 agaacccggc gtccagcgtc gagccggtga cttcctccag gagcggctgc atggtcgtct
     1861 gccgaccgcg gccgtggatg ttctcccgca gccaggccac aagtggctcg aggtttccgg
     1921 cggcgatgtc cgaggcgaga tccggcatga cctttcgggc ggtggcgaac cattgcgcgg
     1981 cggtgagcgc gcccagggta taggtcggga agtaaccgaa aaggcccgcg taccagtgca
     2041 cgtcctgcat gcaaccgtct gcgttgttgc cctcggtgga cagccccaga tagcgctgca
     2101 tggcgtcgtt ccatgcctcg ggcaggtcgg ccacggcgag ggtgccgtcc agcagctggc
     2161 gctccagctc gaaacgcagg atgacgtgga gcgggtaggt gatctcgtca gcgtccacgc
     2221 ggatgtagcc gcgcgccacg cgggtgacgt ggccgtacag gttgtccgtc gaccacgccg
     2281 gctcggtctc gctgcggtcg aagtgccggc ggatcatggg cgccagaaag ccgacgaact
     2341 cgcggctgcg gcagacctgc agctccatga gcagcgactg gctttcgtgg acggccgcac
     2401 ccagtgagtc gccgaccggc tgctgccgcc agtccctggg cagaccctgc tggtagagcg
     2461 cgtgcccggt ttcgtggcag gtcgccatga gcgcctcgat gaagctcgac tcagagtaac
     2521 gggtggtgat gcgggtatcg tccggcacgc cgccgcagaa cgggtgatgg ctgacgtcta
     2581 gcctgccccg atcgaagtcg aagccgagca ggcccatgac ctccaaggac agctgacgct
     2641 gctcctccac cgtgaaaggc ccctcgaggg gcgccacctc gccctggcga tccagggccg
     2701 tctccagcgc gccggggaga aacgccttga gatcctcgaa gacgggctcg atgtcggcga
     2761 tgcgcagctc cggctcgtag acctctaaaa gcgcatcgta gcgggacaga tgcatggcat
     2821 cggccagggc atccgcctgc tgacgggcga gaccgatcac ctccgcgagg aggggcttca
     2881 ccgtctccca gtcgttccgc gcccgggcct cgcgccaggc ctgctcgcat ttcatctgcg
     2941 ccagccggct cgcccgcacc aggtccgaag ggattgccgt ggcttccacg aactcccgct
     3001 ccatgttggt cacgttggcc gattgccagt catcgagctc gaacccctcc ccacgcgcgc
     3061 gattcaac
//
LOCUS       DBNHOX010000243         3817 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1755468, whole genome shotgun sequence.
ACCESSION   DBNHOX010000243 DBNHOX010000000
VERSION     DBNHOX010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1755468"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="AC16UZ_05395"
     CDS             complement(<1..620)
                     /locus_tag="AC16UZ_05395"
                     /inference="COORDINATES: protein motif:HMM:NF017021.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR6246151.1"
                     /translation="MGWLRATLIAVLAVVVALGAMAVWLTQADLSFLKPQVERLIAEQ
                     TGRAFTIGGEFSLNAGRRTAIVAEDVRLGNAEWASYPEMVRIGRLRLELDLLSLLRGP
                     ARIHLIELEDASVLLENPADRDGNWVISADEPDAADSLTLPVLGDIVVDALSVTYLDP
                     ELEGPLSIEVVDARQHFDPETDGLSGSLQATVNGRSVVVSSHVSPLP"
     gene            743..1363
                     /locus_tag="AC16UZ_05400"
     CDS             743..1363
                     /locus_tag="AC16UZ_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_259446992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:785..787,aa:Sec)
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HIR6246152.1"
                     /translation="MTDQLKLEIFSDYIUPWCYLSTGRIEKLKSSFDLEVRWVAFPLH
                     PETPEEGITIEALFAGREIDLDAMYVRMKALMDEEGLPYGRRTHTFNSRLAQELGKWA
                     DSRPGGEAIHDALFRAYFVDGLNIGDAGVLVDIAKSAGLDPGGAREVIDQRTFSNAVD
                     EDWKRSRRYGITGVPTFVAGGYGVVGAQPYETLEHLVQRALTESRA"
     gene            complement(1382..1975)
                     /locus_tag="AC16UZ_05405"
     CDS             complement(1382..1975)
                     /locus_tag="AC16UZ_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6246153.1"
                     /translation="MPDPEQPGELPFRPADAILRPESFLPAADGTEVCAFLNPYDARG
                     AASAHSAGEAISMAAGRIGPGVRSAIHVHPALTQITYVTAGTLTARMRDPGESEPSRL
                     EVPTGAAVLTEAGTLLQLCNETDRTVEVLYVSTPAYVLETAADGAIRYEDALILGADW
                     DAPAVAHWNVETATQARSRATARRAEAAGRIAGRTRR"
     gene            complement(1968..2213)
                     /locus_tag="AC16UZ_05410"
     CDS             complement(1968..2213)
                     /locus_tag="AC16UZ_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246154.1"
                     /translation="MSLIRYPVVQYAYVVDDIEAAGYPAVTTLTSAARVAYMDTRPIL
                     GCFVELYEDNAPLRETFAHWKAVHDAWNGVSDPIRDA"
     gene            2344..3390
                     /locus_tag="AC16UZ_05415"
     CDS             2344..3390
                     /locus_tag="AC16UZ_05415"
                     /inference="COORDINATES: protein motif:HMM:NF053113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-dihydroxypyridine 5,6-dioxygenase NicX
                     family protein"
                     /protein_id="HIR6246155.1"
                     /translation="MLQERIEGKWIDCFARVFERCAVAENEEVAILSESQSRPANAQL
                     AELALLRLGARPYHLVIPTPPQKAPVPVRSTGATDVIGHSRSVVAALSSAGFVVDCTV
                     EGMLHAPELPEILKGGARVLMISNEHPEALERLEPDEALETRVKAGIRKLRGASQMKV
                     TSAAGTDLTVSLEGSVAGGGWGYVARPGIMSHWPGGLCLCFPAAGSVSGVLVLDRGDV
                     NLTFKRYLENPVALSIDADYVTEIDGAGVDAELMRSYFAAWGDQEAYAASHLGWGMNN
                     AARWDAMTMYDRNDFNGTELRAFGGNFLYSTGANEVAGRHTLGHFDLPLRNCSVHLDG
                     EAVVVDGALQRELA"
     gene            3387..>3817
                     /locus_tag="AC16UZ_05420"
     CDS             3387..>3817
                     /locus_tag="AC16UZ_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6246156.1"
                     /translation="MSVPGFKEAGSGEPVVFLHGIGGNRHAFDAQLAEPGRVRSLVLA
                     QTMARFGKPGSDWQQQFLDARLKPLEDGRAPADFARELITGMFGDPSRAEAIERAVAT
                     MSPLPANVYRQVLTCLVVFDQRGTLPEIDAPTLCLAAELDQT"
BASE COUNT          629 a         1257 c         1280 g          651 t
ORIGIN      
        1 ggcagaggac tgacatggga gctgacgacc accgagcgac cattgacggt cgcctggagg
       61 gacccgctga ggccgtcggt ctcagggtcg aaatgctggc gggcgtcgac cacctcgatg
      121 ctgagcggcc cctcgagttc cgggtccagg taggtaacgg acagggcgtc gacgacgatg
      181 tcccccagca cgggcagcgt caacgagtcg gccgcgtcgg gctcatctgc agagattacc
      241 cagttgccat cccggtcggc cgggttctcc aggagcacgg aggcgtcttc caactcgatg
      301 aggtggatgc gtgctggtcc gcgcagcagg gagagcagat ccaactccaa ccggagccgc
      361 ccgatgcgca ccatttctgg gtagctcgcc cattccgcat tgcccagccg tacatcctcg
      421 gcaacgatcg ccgtgcgccg ccccgcgttc agggaaaact caccgccgat ggtgaaagcg
      481 cggcccgtct gctccgcgat caggcgttcc acctggggct tcaagaaact cagatctgcc
      541 tgagtgagcc acacggccat cgccccgagg gcgacgacca cagcgagcac cgcaatcagt
      601 gtggcccgca gccaacccat gaacgcttcc cctcctcgaa cccaagcccg ccggatgacg
      661 ggacgaacga gggatttaac agagatgggc gtggcgggca acgacgggtt acgatggcgc
      721 aaagtcgaag cgggacgggc agatgaccga tcagttgaag ctggagatct tcagcgacta
      781 catatgaccc tggtgttacc tcagtaccgg gcgtattgaa aaactgaagt ccagcttcga
      841 tctggaggtt cgctgggttg ccttccccct gcatccggaa actcccgagg agggaatcac
      901 catcgaagcg ctcttcgccg gtcgcgagat cgatctggat gccatgtacg tgcggatgaa
      961 agcgctcatg gacgaggagg ggcttcctta cggccgtcgt acccacacgt tcaacagccg
     1021 gctcgctcag gaactcggga agtgggccga ctcccgcccc ggtggcgaag ccatacacga
     1081 cgccctgttt cgcgcctatt tcgttgacgg gctgaatatc ggtgatgccg gcgtgctcgt
     1141 cgacatcgcg aagtccgcag gtctcgatcc cggcggtgcc cgggaggtga tcgaccagcg
     1201 gactttctcc aatgccgtgg acgaggattg gaagcgctcg cggcgatacg ggatcaccgg
     1261 cgtgccgacg ttcgtcgccg gtggctacgg cgtcgtcggc gcgcaaccct acgagaccct
     1321 cgagcatctg gttcaacgcg cgctcaccga gtcgcgggct tagctggatc gtcgcggcgc
     1381 gtcagcgcct ggttcgcccg gctatccgcc cggccgcctc ggcgcgtcgc gccgtcgccc
     1441 ggctgcgcgc ctgtgttgcg gtctcgacgt tccagtgggc caccgcgggc gcgtcccagt
     1501 ccgcgcccag gatcaacgcg tcctcgtagc ggatggcgcc gtcagccgcg gtctccagaa
     1561 cgtatgcggg cgtggacacg tagagcactt ccacggtccg gtccgtttcg ttgcacagct
     1621 gcagcagggt gcctgcctcg gtcagcacgg cggctccagt gggcacctcg agtcgggacg
     1681 gttcgctctc ccccggatcc cgcatccgcg cggtgagcgt gcccgcggtg acgtaggtga
     1741 tctgcgtcag cgccggatgg acgtgaatgg cggaacgcac acctggccct atccgcccgg
     1801 cggccatgct gatcgcttcg cccgccgagt gcgcgctggc agcgccgcgc gcgtcgtagg
     1861 gattgaggaa agcgcagact tccgtgccgt cagcggccgg cagaaatgac tcgggtcgaa
     1921 ggatcgcgtc cgctggccgg aagggcagct cgccgggctg ttcagggtca ggcatcgcga
     1981 atcgggtcgc tgacgccgtt ccaggcgtcg tggacggcct tccagtgggc gaaggtctcc
     2041 cgcagcgggg cgttgtcctc gtagagctcc acgaagcagc ccaggatggg ccgagtgtcc
     2101 atgtaggcca cccgggcggc ggacgtcagg gtggtcacgg cgggataccc ggcggcttcg
     2161 atgtcgtcga cgacgtacgc gtactgaacc accgggtagc gtatcaggct cattgctctc
     2221 tcctcgcacg ggtggtgtct ttatacccca atacccccca cccgttaccc acaggggcgg
     2281 ctgccgggac cgctgcggac gccggcaacc cctgtacaat gcggcatttt ggggaaacgt
     2341 cgaatgctgc aggaacgtat cgagggcaag tggatcgact gcttcgcacg ggtgttcgag
     2401 cggtgcgccg ttgctgagaa cgaggaggtc gccatcctct ctgagagcca gagccggccg
     2461 gccaacgcac agctcgccga gctcgccctg ctcaggctcg gggccaggcc ctaccatctg
     2521 gtgatcccta cgccgccaca gaaggcgccg gtgcccgttc gttccacggg cgcaaccgac
     2581 gtgattggcc actcgcgttc cgtcgtcgcc gcgctgtcgt cggcgggatt cgtcgtcgac
     2641 tgcaccgtcg agggcatgct tcacgcgccc gagctgccgg aaatcctcaa gggcggcgcc
     2701 cgggtgctga tgatttccaa cgagcacccg gaggccctgg aacggctgga gcccgacgag
     2761 gccctggaga cccgggtcaa ggccggtatc aggaagctgc gcggcgcgag ccagatgaag
     2821 gtgacctcgg ccgcggggac ggacctcacc gtctctctcg aaggctccgt ggccggcggc
     2881 ggctggggct acgttgcgcg ccccggcatc atgagtcact ggcccggcgg gctttgcctg
     2941 tgtttccccg cggccggctc ggtgagcggc gttctcgttc tcgaccgcgg tgacgttaac
     3001 ctgacgttca agcgttacct ggagaacccc gtggcgctca gcatcgacgc cgactacgtc
     3061 acggagatcg acggggcggg ggtggacgcc gagctgatgc gcagctactt cgcggcgtgg
     3121 ggagaccagg aagcctacgc cgccagccat ctcggttggg gcatgaacaa cgccgcccgc
     3181 tgggacgcca tgaccatgta cgatcgcaac gacttcaacg gcacggagtt gcgagcgttc
     3241 ggcggcaatt tcctatactc caccggcgcc aacgaagtcg ccgggcgcca taccctcggc
     3301 cacttcgacc tgccgctgcg caactgctcc gttcacctgg acggcgaggc cgtggttgtg
     3361 gacggtgcct tgcagcgcga gcttgcttga gcgttccagg tttcaaggaa gcgggttccg
     3421 gcgagccggt ggtgtttctg catggcatcg gcggcaaccg gcatgccttc gacgcccagc
     3481 tggccgagcc agggcgcgtg cgttctctcg tcctcgccca gacgatggcg cgcttcggca
     3541 agccgggcag cgattggcag cagcagtttc tggatgcccg gctcaagccg ctcgaggatg
     3601 gccgtgcccc ggcggacttt gccagagaac tgatcaccgg catgttcggc gatccgtccc
     3661 gtgcggaagc catcgagcgg gccgttgcca ccatgagccc tctgcccgcg aacgtttacc
     3721 ggcaggtgct cacttgcctg gtcgtcttcg atcagcgggg tacccttcct gagatcgacg
     3781 ccccgacgct gtgcctggca gcggagctcg atcagac
//
LOCUS       DBNHOX010000244         2226 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1562879, whole genome shotgun sequence.
ACCESSION   DBNHOX010000244 DBNHOX010000000
VERSION     DBNHOX010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1562879"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="AC16UZ_05425"
     CDS             <1..794
                     /locus_tag="AC16UZ_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR6246157.1"
                     /translation="PAHRENFASMAALTAEKLSIRAGLPADGVLVVEDGVDCSGLSGD
                     IRLRRFGRSEGTAVRLLDCAGGRARYRIGERELEARVPGGGEHRALSLTAVLCVLDTL
                     GRDPASACALPDSLIAPGRGRELVAGGVTVIDDSYNANPESMKAALTALAERSGGRRF
                     ALLGEMLELGDESADYHRTLAPLVEPLDGVWCVGAGMGALRDALPRAKVLGYFDAAED
                     ALTAAVTGHLVTGDTLLVKGSNRVFWARDYVSTLEKSLALSGNNQ"
     gene            822..2054
                     /locus_tag="AC16UZ_05430"
     CDS             822..2054
                     /locus_tag="AC16UZ_05430"
                     /inference="COORDINATES: protein motif:HMM:NF016847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR6246158.1"
                     /translation="MNPQKKARPLDHVFPEVPVRQWVLSFPWPLRLLFASQPDALGRC
                     LSVIIRAIQTNLAHRAGLTASAGARTGVVTLIQRFGSALNLNVHLHMLILDGVYTLER
                     NGPRFHRVGAPDARSLERLLNRLVRRIVRRLTGDGLLIEDPEQPWLDLEPSDTLDHLN
                     AASIRYRIAIGQGAGGRTLTLKNPALVRTNSTPKPFTANQNGFSLNCAVSCQAHQRDR
                     LERLCRYITRPALCLERLSTNAAGQVVYQLKNPFRDGTTHILFSPQDFVARLAALVPR
                     PWVNLTRYHGVFAPSSPMRRAILPTPARARQRRKSKDPATAQATREQQPAATPTDSAD
                     PPTAPLTWAQRLKRVFELDITLCPLCGGQLRVIADITDPDLIRKILDHVQKRAPPGLP
                     PRRAPSDTTYPDLFAKRQ"
BASE COUNT          388 a          792 c          653 g          393 t
ORIGIN      
        1 atcctgcgca ccgggagaac ttcgccagca tggcggcgtt gaccgccgaa aagctcagca
       61 tccgtgccgg ccttccggcg gacggcgtgc tggtggtgga ggacggcgtg gactgcagcg
      121 ggctttccgg cgacatccgt ctgcgccggt tcggtcgcag tgagggtact gcggtgcggc
      181 tgctcgactg cgctggcggt cgggcgcgct accggatcgg cgagcgcgaa ctcgaggcgc
      241 gggtccccgg tggcggcgag catcgtgcgc tgtcgctgac ggccgtgctg tgcgttctgg
      301 atacgctggg ccgggaccct gcttcagcgt gcgcgctgcc ggactcgctg atcgcgccgg
      361 ggcggggccg tgagctggtg gccggcggcg tgaccgtgat cgatgacagt tacaacgcca
      421 atccggagag catgaaggcg gccctgacgg ccctcgcgga gcgttcgggt ggtcggcgat
      481 tcgcgcttct cggcgagatg ctggagctgg gggacgagag cgccgactat caccggacgc
      541 tggcgcccct ggtcgaaccc ctggacggcg tctggtgcgt cggggccggc atgggagcgc
      601 tgcgcgacgc gttgccgcga gccaaggtgc tcggctattt cgatgccgcg gaggatgctc
      661 tgacggccgc ggtaaccgga catctggtca ccggagacac gctgctggtg aaggggtcca
      721 accgcgtgtt ctgggcgcgg gactacgtca gcacgctcga aaaatctctc gccctttctg
      781 gaaataatca gtaaataatt gttatttatg gatttgtctt ggtgaatccc caaaaaaaag
      841 cccggcctct cgatcacgta tttcccgaag tgcccgtccg acaatgggtg ctcagcttcc
      901 cctggcctct gcgcctgctc ttcgccagcc agcctgatgc tctcggacgc tgtctctcag
      961 tcatcatccg agccatccag accaatctgg cccatcgggc gggactcacc gccagcgccg
     1021 gcgcacgcac cggggtcgtc accctgatcc agcgtttcgg cagcgccctg aacctcaatg
     1081 tccacttgca catgctgatt ctggacggtg tctatacgct ggaacggaac ggaccgcgat
     1141 tccatcgcgt cggtgcaccc gacgcgcgga gcctcgagcg tctcctcaat cggctcgtcc
     1201 ggcgcatcgt ccgcaggctc accggcgacg ggctcctcat cgaagatcct gaacagccct
     1261 ggctcgatct ggagccctca gacacgctgg atcatctgaa cgccgcctcg atccgttacc
     1321 ggatcgcgat cggccaaggc gccggcggca gaaccctcac cctcaagaac ccagccctgg
     1381 tccggaccaa ctcgacaccc aagcccttca ccgccaatca gaacggcttc tccctgaact
     1441 gtgccgtctc ctgtcaggcc caccagcgcg atcggctcga gcggctgtgc cgctatatca
     1501 cccggccggc gttgtgtctc gagcggctgt caacgaatgc cgccgggcag gtcgtctatc
     1561 aactcaagaa cccgttccgc gacggcacca cacacatcct tttcagcccg caagacttcg
     1621 tcgcccgatt agctgctctc gtgccacgcc cgtgggtcaa tctcactcgc taccacggag
     1681 tcttcgcccc gagctcgccc atgcgccgcg cgatcttgcc gactccagca cgagctcggc
     1741 agagacgaaa gtccaaagac cccgccactg ctcaggcaac ccgggagcag caacccgcag
     1801 ccactcccac cgactctgct gatccgccca ccgcgccgct gacctgggcg cagcgcctga
     1861 aacgagtgtt cgaactagac atcaccctct gcccgctctg cggcggacag cttcgcgtga
     1921 tcgcagatat caccgatcct gacctcatcc gcaagatcct cgaccacgtt caaaagcgtg
     1981 ccccaccagg gctaccgcca cgacgcgcac cttccgatac aacctatccc gacctgtttg
     2041 ccaaacgcca ataaatccga ggcgaccatc ccggctgcgc gtaccatctc tcggcttccc
     2101 cgttcacccc gacacccgta cgacaaccgc tactaccgcc gtgcgatctc caagccatcc
     2161 ttatagatga ggatcagctg gcccagcgcc gcccgctacg ccaactcctg ccatacgcct
     2221 ggcaac
//
LOCUS       DBNHOX010000245         6625 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_920408, whole genome shotgun sequence.
ACCESSION   DBNHOX010000245 DBNHOX010000000
VERSION     DBNHOX010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6625)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_920408"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="AC16UZ_05435"
     CDS             <1..195
                     /locus_tag="AC16UZ_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010797309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL13 family ribosomal protein"
                     /protein_id="HIR6246159.1"
                     /translation="GYPGGIKGISFEHLQQKHPERIIEKAVKGMLPRNTLGRAMFRKL
                     KVYAGPEHPHSAQQPEPINL"
     gene            230..622
                     /gene="rpsI"
                     /locus_tag="AC16UZ_05440"
     CDS             230..622
                     /gene="rpsI"
                     /locus_tag="AC16UZ_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR6246160.1"
                     /translation="MSANQFYGTGRRKTSAARVFISSGTGNIFVNNQSLDSYFGRETS
                     RMIVRQALFVADVADKVDIRVTVRGGGNSGQAGAIRHGIARALVAHDESLRGALRKAG
                     FLTRDAREVERKKVGLRKARKRPQFSKR"
     gene            753..1403
                     /gene="petA"
                     /locus_tag="AC16UZ_05445"
     CDS             753..1403
                     /gene="petA"
                     /locus_tag="AC16UZ_05445"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HIR6246161.1"
                     /translation="MSADKAATDSAAVPKSLDPSKVNKERRYFLIGASAVVGGIGIVG
                     AAVPFVASWQPSAKARALGAPVTVDISKLRPGEILGPTPAWRGNPVFVVYRDEDALRR
                     LEQENDRLADPDSDNAEQQPDYAKNPWRSKRPEIGVYLGLCTHLGCSPKYHGEVKPEP
                     FAADWEGGFFCPCHGSLFDLAGRVVKGVPAPDNLPVPPYQFVSDTVVVIGVDEETA"
     gene            1403..2707
                     /locus_tag="AC16UZ_05450"
     CDS             1403..2707
                     /locus_tag="AC16UZ_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017132656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR6246162.1"
                     /translation="MEQSQPGRIARGWSNFMEWVDERFPLTETFEYHMSRYYAPKNFN
                     VWYLFGVLSMVVLVIQLLTGIWLTMSYVPSGDGAFASVEYIMRDVEYGWLLRYLHTTG
                     ASAFFLVVYLHMFRAMMYGSYRKPRELLWLIGMAIFACLIVEGFLGYLLPWGNMSYWA
                     AQVIISLVGAIPVVGPDLMEWVRGDFLVSDITLNRFFALHVVALPLVLVMLVFVHIVA
                     LHHVGSNNPDGIEIKQNKDENGIPLDGVPFHPHYTIGHDLPAIVLFLFFFCIVVFYVP
                     EMFGYFIEKPNFEEANSLKTPEHIAPVWYYTPFYAMLRAATFPLFGIPAKFWGFVVMA
                     GAIVLPAVLPWLDRSPVKSIRYKGWLSKGMLALFVISFFILGYLGTIAPSKPATALAQ
                     ICTVVYFAYFVLMPWYTRVEKTKPVPDRVTTKATRAAKAEGA"
     gene            2711..3433
                     /locus_tag="AC16UZ_05455"
     CDS             2711..3433
                     /locus_tag="AC16UZ_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HIR6246163.1"
                     /translation="MKSRSSFLWALTAATLTFSAGAFPAGSGWPLDSIDPDLEDLPSL
                     QNGFKLYVNYCIGCHSVKYQRYERTADDLGIPHDIAMDNLVLTGQKIGELMTTSMDPE
                     LAKNWFGAPPPDLTMVARVRGTDWLYTYLRTFYQDEARPFGVNNLVFENVGMPNVLAG
                     LQGEQVLDDSGSPELVAGTGQLSPEEFDQVIYDIVNFLYYTGDPSRLERHRTGIYVLL
                     FLVVLYVFTYLLGREYHKEIVD"
     gene            3488..4120
                     /locus_tag="AC16UZ_05460"
     CDS             3488..4120
                     /locus_tag="AC16UZ_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6246164.1"
                     /translation="MSVVTKRSSMTFFSDPRDHYCHRVRMVLAEKGVTVDIVDVDPSR
                     KPEDLAEINPYNTLPTLLDRDLVLYEANVIMEYLDERFPHPPLLPVYPVQRALSRLWI
                     TRVEKEWSGRLDILMAGKGRETTITKARKELRESIISIAPIFSEKPFFMNDEFTLVDC
                     CVAPILWRLKYVGINLPERSTRPLQRYMQVMFERSSFRESLSEAELEMRD"
     gene            4139..4531
                     /locus_tag="AC16UZ_05465"
     CDS             4139..4531
                     /locus_tag="AC16UZ_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HIR6246165.1"
                     /translation="MKAKAKRPYLLRALYDWLVDSSLTPYVLVSAEDEAAMVPADYVN
                     DGKIVLNISPSAVRGLVIDDAGLSFDGRFGGAPYPVSAPISSVLALYAKETGEGMIFD
                     PEEFPSRTDDSPEAPKDRPSGPELKVIK"
     gene            complement(4584..4949)
                     /locus_tag="AC16UZ_05470"
     CDS             complement(4584..4949)
                     /locus_tag="AC16UZ_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR6246166.1"
                     /translation="MTPQARAGAGAESAALDHLRARGLELVARNFRCRLGELDIVMVD
                     GNELVFVEVRLRNHARFGDGAASITAAKRQKLARAARAFLQRHPRYQSCPCRFDVVAI
                     KGEGQRMEMRWLKNAFAVG"
     gene            complement(4946..>6625)
                     /locus_tag="AC16UZ_05475"
     CDS             complement(4946..>6625)
                     /locus_tag="AC16UZ_05475"
                     /inference="COORDINATES: protein motif:HMM:NF016251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HIR6246167.1"
                     /translation="SSPEALLQQADLANGREAARLYLSATESFIQANRLDEAARSFQR
                     IDQALLSGARTADYRYAAAEIALHDGEPDRAAALLGAGEPGGGSARRRGLWAATLPGR
                     ICHAAGDDQCAIRELTGLEVPGDQRQELHDVIWRYLGFAAGFSAPAQAGRTSGREQGW
                     WALKATVLQGISVEDQRQRVDDWRGAWPDHPAASVLPTALRAFDRDAPKVRRVGLLLP
                     LSGPLAVAGRAVRDGYIAAYLHESAQSEQEIIVYDTAAAPLPSLYEKALLDEVDVLIG
                     PLTKEEASALSGLAPSVPTLVLNYLEDGQRAPSNVYQLGLAIEDEADTMVSRLLEDGA
                     ERLLVVHNDQDWSRRAARRLTSSWPYPVTREFFPDVKTITESVGRAMQIDASLERRDE
                     LASLLGTDLEFLPRARGDLDAVVALVDTVEANALVPALRFHFAQDLPVYASSQTVRGA
                     TAQELGELAGFRVSELPWILFSDPIYLAMADVFLLQGNRFAALYALGVDAYRVSGRLT
                     LLASGGVHQLLGSTGLLTVDGSGRFRRELAWGLIRRGRLEPLPVMAGQSAL"
BASE COUNT         1183 a         2119 c         2105 g         1218 t
ORIGIN      
        1 ggctatcccg gcggcatcaa ggggatcagc ttcgagcatc tgcagcagaa gcacccggag
       61 cgcatcatcg agaaagccgt caaggggatg ctgcctcgca acacgctcgg ccgggccatg
      121 ttccgcaagc tcaaggtcta cgccggcccg gaacatccgc acagcgccca gcaacccgaa
      181 ccgatcaacc tatagccagt tagccgatca ggcccaggac gcacacagca tgtcagcaaa
      241 ccaattttac ggaacggggc gacgcaagac ttccgcagcc cgcgttttca tctcttcggg
      301 caccggcaac atcttcgtca acaatcagag cctcgacagc tatttcgggc gcgagacgtc
      361 ccgcatgatc gttcgccagg cgctctttgt cgccgacgtc gcagacaagg tggacattcg
      421 cgtgaccgtt cgcggtggag gcaactcggg ccaggctggc gcgatcaggc acggcatcgc
      481 ccgggcgctc gttgctcacg acgaaagcct tcgaggtgcg ttgcgcaagg ccgggttcct
      541 gacccgcgac gcgcgcgagg tggagcgcaa gaaggtcggt ctgcgcaagg cgcgtaaacg
      601 cccgcagttc tccaagcgat agaggcgtcg gcgcgccgtt cgggccccgt ccacaaggac
      661 ctccgcgcgg gctgcgcgag cctcctgcgg gcaaggatgt gcacccgcgt gtgctagaat
      721 gcggggctgt ttgaatatgc tgggagcgac ccttgagcgc tgataaagct gcaacggatt
      781 ctgccgccgt tcctaagagt ctcgaccctt ccaaggtcaa caaggaacgc cgctactttc
      841 tgatcggagc ctccgccgtg gtaggtggta taggcatcgt gggcgcagcg gtgcccttcg
      901 tggcgtcgtg gcaacccagc gcgaaagcgc gtgcgctcgg tgctccggtg accgtggata
      961 tcagcaagct tcggcccggt gaaatactgg gacccacccc ggcatggcgg ggtaacccgg
     1021 tgttcgtggt ctaccgcgac gaggacgcct tgcgcaggct cgagcaggag aacgaccggc
     1081 tcgccgaccc ggattccgac aacgccgagc agcagccaga ttacgcaaag aacccgtggc
     1141 ggtcgaagcg tccggagatc ggcgtctacc tggggctgtg cacgcacttg ggttgctcgc
     1201 cgaaatacca tggtgaggta aagccggaac cctttgccgc tgactgggaa ggcggcttct
     1261 tctgtccgtg tcacggatcc cttttcgatc tggcgggacg ggtcgtcaaa ggggtcccgg
     1321 cgccggacaa cctgccagtg cccccgtatc agttcgtgag cgacacggtg gtggtgatcg
     1381 gcgtggacga ggagacagcg tgatggagca atcgcaaccg ggaaggattg cgcgcggctg
     1441 gtccaacttc atggaatggg tcgacgagag gtttccgctc accgagacct tcgagtacca
     1501 catgtcccgg tactacgcgc cgaagaactt caatgtctgg tatctgttcg gcgttctctc
     1561 gatggtcgtt ctggtgatcc agctgctgac cggcatctgg ctgaccatga gctacgtgcc
     1621 cagcggcgac ggcgctttcg cttccgtcga gtacatcatg cgcgacgtcg agtacggctg
     1681 gctcctgcgc tatctgcaca ccaccggggc ctcggccttc ttcctggtcg tttacctgca
     1741 catgttccgg gccatgatgt acggctccta tcgcaagccg cgggaactgc tctggctcat
     1801 cggcatggcg atatttgcct gcctgatcgt ggaagggttt ctcggctacc tgctgccctg
     1861 gggcaacatg tcctactggg ccgcccaggt gatcatctcg ctagtcggcg cgattcccgt
     1921 ggtcgggcct gatctgatgg agtgggtccg cggcgacttt ctcgtctcgg acatcacgct
     1981 gaatcgcttt ttcgcccttc acgtggtggc cctgccgctc gtgctggtga tgctcgtctt
     2041 cgtgcacatc gtcgctctgc atcacgtcgg ctccaacaac cccgacggta tcgaaatcaa
     2101 gcagaacaaa gacgagaacg ggattcccct ggacggggtt cccttccatc cgcactacac
     2161 catcggacac gatcttccgg ccatcgtgct ctttctcttc ttcttctgca tcgtggtttt
     2221 ctacgtgcca gagatgttcg gctacttcat cgaaaagccg aacttcgagg aggccaactc
     2281 gctgaagaca cccgagcaca tcgcgccggt gtggtactac acgccgttct acgcgatgct
     2341 gcgcgcggcc acgttccctc tcttcgggat tcccgccaaa ttctggggat tcgtagtcat
     2401 ggccggcgcc atcgtgctgc ccgcagtcct tccgtggctc gaccgcagcc cggtgaaatc
     2461 catccgctac aaggggtggc tgtccaaggg gatgctggcg ctgttcgtga tcagcttctt
     2521 catactcgga tacctgggaa ccatcgcgcc gtcgaagcca gcaacggctc tcgcccagat
     2581 ctgcaccgtc gtctacttcg cgtacttcgt gctcatgccc tggtacacgc gggtggagaa
     2641 gacgaaaccg gtgccggaca gggtgactac caaggcgacg cgagcggcaa aggccgaggg
     2701 ggcgtaaggc atgaaatcac gcagctcgtt tctctgggcc ctgaccgccg cgacgctgac
     2761 attctctgca ggcgcgtttc ctgccggctc gggctggccg ctggacagca tcgatcctga
     2821 tctggaggac ctgccttccc tgcagaacgg cttcaagctt tacgtcaact actgtattgg
     2881 ctgccactcg gtgaagtatc agcgctacga gcggacggcg gatgatctgg ggattcctca
     2941 cgacatcgcg atggacaacc tggtcctgac cggccagaag atcggagagc tgatgaccac
     3001 cagcatggat ccggagctcg ccaagaactg gttcggcgcg cctcccccgg atctgaccat
     3061 ggtcgcgcgg gtgcgcggca cggactggct gtacacgtac ctgcgaacgt tctaccagga
     3121 cgaggcgcgg cccttcggag tcaacaacct ggtgttcgag aacgtgggta tgcccaacgt
     3181 tctggccggc ctccagggag agcaggtgct ggatgattcc ggttcacccg agctggttgc
     3241 aggcaccggg caactctcgc ccgaggagtt cgatcaggtg atctacgaca tcgtgaactt
     3301 cctctactac acgggggatc cgagccgtct ggaacgtcac cgtacgggaa tttacgtgct
     3361 gctgtttctg gtagtgcttt acgtgttcac gtatctcctc ggccgcgaat atcacaagga
     3421 aatcgtcgac tagatcgttg ggggccggca ggttcttgta acgcgaattt cagacgaaag
     3481 gtaaatcatg agcgtagtca caaagcggtc ctcaatgacg ttcttctccg acccccggga
     3541 ccactactgc caccgggtgc ggatggtgct cgccgagaaa ggcgtgaccg tggatatcgt
     3601 ggacgtggat ccgtcgcgca agccggagga cctggccgag atcaatccgt acaacacctt
     3661 gccgacgctg ctcgaccgcg acctcgtgct ctacgaggcc aacgtgatca tggaatatct
     3721 ggacgagcgg tttccgcacc cgccgctgct gcccgtctat cccgttcagc gcgctctgtc
     3781 gcggctttgg atcacgcgtg tggagaagga gtggagcggc cggctggata ttctgatggc
     3841 cggcaaaggt cgcgagacga ccatcaccaa ggcgcgcaag gaactgcgcg aaagcatcat
     3901 tagcatcgcg ccgattttca gcgagaagcc ttttttcatg aacgacgagt tcacgcttgt
     3961 cgactgctgc gtggcgccca tcctgtggcg attgaagtac gtcggcatca acctgccgga
     4021 acgctcgacg cgtcccctgc agcgctacat gcaggtcatg ttcgagcgca gctcgttccg
     4081 agaaagcctc tccgaggccg agctcgagat gcgggactga ccccgggggc ggtctggcgt
     4141 gaaagccaag gcgaaacgtc cttacctgct tcgagccctt tacgactggc tcgtcgactc
     4201 ttcgctcacc ccgtacgtgc tcgtctccgc ggaggacgaa gcggcgatgg tgccggccga
     4261 ctacgtcaac gacggcaaga tcgttctcaa catatcgccg tcggccgtgc gaggacttgt
     4321 catcgatgac gccgggctca gcttcgacgg gcggttcggt ggagcgccct atccggtgtc
     4381 cgcgcccatt tcgagtgttc tggccctcta cgccaaggag accggcgagg gcatgatctt
     4441 cgacccggag gagtttcctt cccgtaccga tgattccccg gaagcgccga aggaccgccc
     4501 gtccggtccc gagctgaagg tcatcaagta gcggctgcat ttctcattgc ttcaccgcgc
     4561 cagctggcgg caggatcgaa tcattacccg accgcgaacg cgttttttag ccatcgcatc
     4621 tccatgcgtt gaccctctcc tttgatggcc accacgtcga accggcaagg gcagctctgg
     4681 tatcgggggt ggcgttggag aaatgcccgg gcggcccttg ccagcttctg ccgctttgcg
     4741 gcggtgatgc tcgccgcgcc gtcgccgaag cgagcgtggt tgcgcagacg gacttcgacg
     4801 aaaaccagct cgttgccgtc caccatgacg atgtccagct cgcccagacg gcagcggaaa
     4861 ttgcgggcta ccagctcgag gccccgcgcg cggagatggt ccagcgcggc cgattctgcc
     4921 ccggcacctg cccgtgcctg gggcgtcaga gggccgattg cccggccatc accgggagcg
     4981 gttcgaggcg gcccctgcgg atcagtcccc acgccagctc ccgacggaac cgtccggagc
     5041 catccaccgt gagcaggccc gtgctgccca gcagctggtg gaccccgccg gaggccagca
     5101 gcgtcaggcg cccgctcacg cgataagcgt ccacgccaag cgcgtacaag gcggcgaagc
     5161 gattgccctg caggaggaac acgtcggcca tggccaggta gatggggtcg gagaacaata
     5221 tccagggcag ctcgctgacg cgaaacccgg caagctctcc gagttcctgt gccgttgctc
     5281 cacgcaccgt ctgcgagctc gcgtacacgg gcagatcctg ggcaaaatga aagcgcaacg
     5341 caggcacgag ggcattggcc tcgacggtat cgacgagcgc gaccacggcg tcgaggtccc
     5401 ccctggcgcg tggcagaaac tccaggtcgg tccccaggag actggccagc tcgtcgcggc
     5461 gctcgaggct cgcgtcgatc tgcatggccc gtccgacgga ttcggtgatg gttttgacgt
     5521 ccggaaagaa ctcgcgcgtg accggatagg gccagctgga agtaagtcgc cgcgccgctc
     5581 gacgcgacca atcctgatcg ttgtggacga ccagcaagcg ctctgcgcca tcctccagga
     5641 gcctgctcac catggtgtcg gcctcgtcct cgatggcaag cccgagctgg tagacgttcg
     5701 agggcgccct ctggccgtct tcgagatagt tgagcaccag ggtgggaacg gacggggcga
     5761 gcccgctgag ggcggacgcc tcttccttcg tcaggggccc tatgagcacg tccacctcgt
     5821 ccaggagggc cttctcgtac aagctcggca gcggtgcggc ggccgtgtcg tagacgatga
     5881 tctcctgctc gctctgggca gactcgtgca gataggcggc gatgtagccg tcccgaacgg
     5941 cgcgcccggc aacggcgagg gggccgctga gcggcagcag caacccgacc cgtcgcacct
     6001 tcggggcgtc ccggtcgaag gcgcggagag ccgtgggcaa gacggaagct gccgggtgat
     6061 cgggccaggc gccgcgccaa tcgtccacac gctgacgctg gtcttccacg gagatgccct
     6121 gcagcaccgt ggccttgaga gcccaccaac cctgctcgcg cccggaggtg cggccggcct
     6181 gagcgggcgc ggagaaaccg gctgcaaaac ccagatagcg ccagatgacg tcgtgcagct
     6241 cctggcgctg gtcgccgggt acctcgagtc cggtcagctc ccggatcgcg cattggtcat
     6301 cgccggcggc gtggcagatg cggccgggca gggtggccgc ccacagaccg cgccgtcgcg
     6361 ccgagccgcc gcccggctcc ccggcgccaa gcagcgcggc cgcccgatcc ggctcgccgt
     6421 cgtgcagcgc gatctcggcg gcggcatatc gatagtcggc cgtccgggcg ccggagagca
     6481 acgcctggtc gatcctctgg aaagatcggg cagcctcgtc gagccggttg gcctgaatga
     6541 agctctcggt ggccgacagg tacagccggg ctgcttcccg cccgttggcc agatccgcct
     6601 gttgcaggag agcttcggga gatga
//
LOCUS       DBNHOX010000246         1728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_341841, whole genome shotgun sequence.
ACCESSION   DBNHOX010000246 DBNHOX010000000
VERSION     DBNHOX010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_341841"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="AC16UZ_05480"
     CDS             <1..706
                     /locus_tag="AC16UZ_05480"
                     /inference="COORDINATES: protein motif:HMM:NF023358.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HIR6246168.1"
                     /translation="SAASLNDIFVVTDRLNTQAKRSQAKIDALTEETRKLLGEYKTVL
                     KEIEGLRVYNRQLEKQISSQEAEMAKLASSIDQVTLIERQITPLMLRMIDGLEQFVEL
                     DLPFLQDERAGRIEALREMMDRADVAVSEKFSQILRAYQIENEYGRTMEAYGDTINVA
                     GVDLKADVLKVGRVTLVYQTPDGASTGRWDPKAGQWVELGDEYTTPVRSGIRMARKQL
                     SVDMLTMPVAAPEAAE"
     gene            706..>1728
                     /locus_tag="AC16UZ_05485"
     CDS             706..>1728
                     /locus_tag="AC16UZ_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246169.1"
                     /translation="MNHVKIKIGAGILALGLNGAALAEEAAPDVVVNQADTLEELLRN
                     VEERRVTESREHTQREQVFVREKNQQARMLADAQAERRREEQRSERLETAFEENEIRI
                     GDLQEQLDRRLGSLRELFGVLQQVAGDTRGLFEGSIISAEFPNRGEWLGQLAAKMGTA
                     SELATIDEMETLWFELQREMIESGKVSKFPGTIIQLDGQRKDADIIRVGAFALISEGE
                     YIQFDVNDGSLTELARQPSGRYTGSAEDLQEAGAGDVVAFAVDPTRGSLLSLLIQAAT
                     VGERVGSPFGGIAKGECYLPFCDGQGGYVGAVIILGGLIGVLLAVERLVTLSMVGAKV
                     ASQKKSS"
BASE COUNT          357 a          488 c          576 g          307 t
ORIGIN      
        1 gtcggcggca tcgttgaacg acattttcgt ggtgaccgat cgcctcaaca ctcaggcgaa
       61 gcgatcccag gccaaaatcg acgcgctgac cgaggagact cgcaagctgc tcggtgagta
      121 caaaaccgtg ctcaaggaaa tcgaaggcct gagagtctac aaccgacagc tcgaaaagca
      181 gatttccagc caggaggccg agatggccaa gctggcaagc tccatcgacc aggtgacgct
      241 catcgagcgg cagatcacgc ctctgatgct gcgcatgatc gatgggctag agcagttcgt
      301 ggagctcgat ttgccgtttc tgcaggacga gcgtgcgggc cggatagaag ctctgcgcga
      361 gatgatggat cgggcggacg tggccgtatc cgaaaagttc agtcagatac ttcgcgccta
      421 tcagatcgag aacgagtacg ggcgcaccat ggaagcttat ggagacacga tcaacgtagc
      481 gggtgtggat ctcaaggcgg acgttctcaa ggtggggcgg gtgacgctcg tgtatcagac
      541 gcccgatggg gcctccaccg gccggtggga cccaaaggcc ggacagtggg tcgagttggg
      601 agacgagtac accaccccag tacgaagtgg tatccggatg gcgcgcaagc agctctccgt
      661 tgacatgttg acgatgcccg tcgcggctcc ggaggctgca gagtgatgaa tcacgtaaag
      721 atcaagatag gcgctggaat tctggcgctc gggctcaatg gcgctgctct tgccgaagag
      781 gccgcgccgg acgtggtcgt aaaccaggcc gatactctcg aagagctcct gaggaacgtc
      841 gaggagcgca gggtcacgga atcccgtgag catactcagc gcgagcaggt gttcgttcgc
      901 gaaaagaacc agcaggcgcg catgctcgcc gatgctcagg cggaacgccg acgtgaggag
      961 cagcgttcgg aaaggctgga aaccgccttc gaagagaacg agatacgtat cggcgacctc
     1021 caggaacagc tcgaccggcg cttaggctcc ctgcgcgagc tttttggcgt tctccagcag
     1081 gtagccggcg atacccgcgg gttgtttgaa ggctcgatca tctccgccga gttccccaac
     1141 cgtggagaat ggctcggcca gctcgcggcc aaaatgggca ccgcgagcga gctggcgacc
     1201 atcgacgaga tggaaaccct ctggttcgag ctccagcgtg aaatgatcga gtcgggcaag
     1261 gtctccaagt ttcccggaac catcatccag ctggacgggc agcgtaagga cgccgacatc
     1321 atccgcgtgg gtgcctttgc tctgatctct gaaggcgagt acatccaatt cgacgtcaac
     1381 gacggtagtc tgaccgagct ggctcggcag ccgtccggcc gctacacggg ttcagccgaa
     1441 gatctgcagg aggccggtgc cggcgacgtc gtggccttcg cggtggaccc gactcgtggc
     1501 tccctgctgt ctctgctgat tcaggccgcg accgtgggcg agcgggtggg ttctccgttt
     1561 ggcggcatcg ccaagggcga gtgctacctg ccattctgcg acggtcaggg tggctacgta
     1621 ggcgccgtga tcattctggg cgggctgatc ggcgtgctgc tcgccgtgga gcggctggtc
     1681 acgctctcaa tggtgggcgc gaaggtcgcc tcgcagaaga aatcgagc
//
LOCUS       DBNHOX010000247        10632 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1370080, whole genome shotgun sequence.
ACCESSION   DBNHOX010000247 DBNHOX010000000
VERSION     DBNHOX010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10632
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1370080"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1504)
                     /locus_tag="AC16UZ_05490"
     CDS             complement(<1..1504)
                     /locus_tag="AC16UZ_05490"
                     /inference="COORDINATES: protein motif:HMM:NF025114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6246170.1"
                     /translation="MNDEPDVLVVGAGFAGMYAVHRLGELGLTVKVLEAGGDVGGTWY
                     WNRYPGARCDVPSLEYSYGFSPELEQEWDWPEVFSSQEDILAYANHVADRFDLRRHMR
                     FDTRIVAVEYREDENVWRLTSEAGEVFEAPFCVMATGCLSVPNTPRLPGADSFAGLVL
                     HTGRWPHEAVDLAGKRVGIIGTGSSGVQAIPELARQSGHLVVFQRTPVYTVPANRKAM
                     RPDVQREFREHYRSIRGMQQRNAGGVSGYRPLRGGRAGPAVPGERALRTGILDLDPDQ
                     RRRIVAERGLETLLNFPDVYTDPEANEVANALYRDAVRAEVRDPETAEKLLPKDYGLG
                     CKRQVLDRDFFETFNRDDVTLVDLRETPMETLNPDGIRTSHGQYDLDVLIYATGFDAM
                     TGALTRANVRGRDGQALADKWRYGPVAYLGLQMRGFPNLFTVTGPGSPSVLCNMLVAI
                     EQHVNWIGECITWLRETNRTRIEPLAESETEWVAHVNEVAEGTMYTAPSCN"
     gene            1652..2260
                     /locus_tag="AC16UZ_05495"
     CDS             1652..2260
                     /locus_tag="AC16UZ_05495"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6246171.1"
                     /translation="MLILHHAPNSRAGRIVWLLEELGLDYELNRMDFHPRDLKSEAHR
                     ERHPLGRVPVLEDGEIMLYESGAIVEYIVARHSDGALKPSVDSPLFPEYLQWFHYCEG
                     MVMPPVNTIVVQTLLLPPERRNEEALGQAQRLLAKAVAPVDEALAGREYLIGDFSAAD
                     IMLGHALYMSRRLGAVSEEMTNLNAYIDRIEARPAFQKGIQA"
     gene            2292..3587
                     /locus_tag="AC16UZ_05500"
     CDS             2292..3587
                     /locus_tag="AC16UZ_05500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spinster family MFS transporter"
                     /protein_id="HIR6246172.1"
                     /translation="MSGARSHWSVGGRNYVLVLLTATAAFNFVDRQILGVLLEPIGQE
                     FDLPDSLLGLLSGIAFAVFYAVLGIPIAALADRLNRRNIIVVCIALFSTMTALCGAVV
                     NFWQLFLARIGVGVGEAGTMPASQSMLSDLFPVQERPWAMGLLATGGNIGLMLGLLIG
                     GWVNEWFGWRAAFVTAAVPGALIAAVIYFTVPEPVRRHAAAARSGWMREAFTGMRLVG
                     SIRSVRRFAMGGTLYGLSAYGIHTWMPVYYIRYHGLSTGEAGTVISIIVGVLGGIGAF
                     AGGVFCAKLSQRDLRWNGWLPALAIFTSVPLLIAMLITDNTWLAIGLFVLPGILSSVY
                     AGPTWSIIQELVPAQRRAMAASVYMLFFNLIGLGLGPLTVGVLSDLYIPAIGEASLRW
                     AMVSVLLVSLGGITAYVLAARSLIGDLDVLRSRAGGAQT"
     gene            3584..4585
                     /locus_tag="AC16UZ_05505"
     CDS             3584..4585
                     /locus_tag="AC16UZ_05505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6246173.1"
                     /translation="MTVNTQVLIDSLPQGKLTADNYRIAESPVPEIGDGEVLVQTTAF
                     AITAGTRAGLQGSASYAGAPQTGVVMNGTGVGEVTESRSEDFAAGDRVLGPTGWQAYS
                     VHKPRALTLIPEGHDPVDYLGPLGVNGLTAYFGLFEVGRPAAGETVMISAAAGSVGHL
                     VGQMATTVDARVVGVCGSDAKGRILTDELGFDAVVNYREDGFRQALKEATPDGVDVYF
                     DNTGGDILGAALRRMNVAGRIACCGVVSQYDTDNPEPGPRGIPGLLINKRLDMRGFLV
                     FDFADRYDEARGNIAAWLESGKLKALVDEVDGVEAAPAAFVDLLAGGNVGTRVVRVT"
     gene            4606..5952
                     /locus_tag="AC16UZ_05510"
     CDS             4606..5952
                     /locus_tag="AC16UZ_05510"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6246174.1"
                     /translation="MAETNIQSAPQESRAEKLDAGHPQFLRGDPITGDRYYSQDFARR
                     EWDRMWTRVWHIAGRESELPDAGDWIVHNFLRESIIATRQSDGSIRAFYNSCQHRGNR
                     LVWAGEGRGNFTCSYHGWEYGTDGVLVHAQDPEDFDGGSPCGRVSLVEVPCDTWGGFV
                     WYTMDPEAPSLAAFLDPIPELFANRDLDAAVRVVRRAFTVDANWKFASDNFNESYHLP
                     TVHPELATTTDEDYRNTIFEAYSTGHNRMIEQGQPSMRAPRPNEVEAPWDEILAAWDL
                     DPADFAARARDGRIALQQQRRKLGPARGYGHYAKYSDDELTDYFHHTLFPNVTLTATA
                     DGVHFFRTEPHPADPEKCTFEYWFFASPVEGQDEVVTICGPRPLQEAEPEQFEYGSGA
                     VIEDMMDSFLIQDLGVAVGQQQGFHSRGYRDAILSGQESRVRRFHEVLNDYLEGRR"
     gene            complement(6002..7321)
                     /locus_tag="AC16UZ_05515"
     CDS             complement(6002..7321)
                     /locus_tag="AC16UZ_05515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6246175.1"
                     /translation="MRDRFSQAYLWYVVAVLTIASTFSIIDRQILNVMIGPVKRDLGG
                     ISDFQVSLIMGFAFTFLYSVLSYPAGWVADRYNRKNLMAVGIAAWSAMTMLCGMVSQY
                     WHLFATRMGVGVGEATLQPAATSSLSDYFPPHMLPLAMGIVASAPFIGQGLANIAGGP
                     LIDYLEATPNYVLPLFGEVFSWQMVFLLVGAPGLLVALAMWGLREPSRHGKQTEEAGV
                     PLREVWAFVKDRGAFFFLVFTAYLCLATQGWSLFSWLVEFYVRNHEWTRTEIGLTYGF
                     IAMFVGMAGSIIGGAWAGRLIRRDSPDGTIRVVMYGSIVLLPVGAAITIVPDPWLGVA
                     LLVPATFCMAMPPGLIMATLQAIAPNELRGQMVAFYLIAVNFLAYTFAPSLPAVLSDF
                     VFGSEMALGKSISLLAVVNYTISSLCLALGLKHYRRALDRARAWASA"
     gene            complement(7339..8118)
                     /locus_tag="AC16UZ_05520"
     CDS             complement(7339..8118)
                     /locus_tag="AC16UZ_05520"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246176.1"
                     /translation="MNLKGQVAVISGAAVGIGRGIAVALAEQGAAVACLDIDGVGNQE
                     TAGLAEEAGGRGLALDCDVADRAQVRRAMSEALGALGRIDVLVNNAAIYVDTSLTVGS
                     YDAQTRAYERSIAICALGSYYCARAAVPAMRESGGGNIVNIITEHIKEGHLMTGPGAT
                     GYDGAKWVMWRQTESWAVELAEHNIRVNALCMGATDTPMLRAVSVPIAEAGMRPEDVG
                     QAVLNVLAHGRRGPTGQSYVFGTTGTSREQSLGEIAALAPR"
     gene            complement(8134..8547)
                     /locus_tag="AC16UZ_05525"
     CDS             complement(8134..8547)
                     /locus_tag="AC16UZ_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6246177.1"
                     /translation="METAVKLAIHDLLGRSAYALDERHVDMLEGTFTEDAAFEIDIQG
                     VDGTMSFAGREAIMGLMTDSMAEQTDQRRHVVTNVFFEEVGDERARLISNITITSVEH
                     GQIRLVTSGLYRDEVVLHNGDWRIARRRIELDMPY"
     gene            8733..10073
                     /locus_tag="AC16UZ_05530"
     CDS             8733..10073
                     /locus_tag="AC16UZ_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6246178.1"
                     /translation="MQRLKNSTLFYYSLTDLPIAMSMFPVAVFIPRFYASDVGVPLAV
                     VGTIILVVRIFDVVTDPLMGYLSDQTRTRFGRRKPWIFAATPIMMTSIYMLFMPPEDA
                     GWLHMFTWSMLLSIAITMMLIPYYAWGAELSPDYNERSRITGGRAMAGVFGSLSAQLI
                     PAGALLFFGLGGSDVVLTLVGTTMLVLMPICVLLTLLNTPEPEVRHKSTVPVGKGLKL
                     MWANGPFKQLVLAFMIGSIGLNITTPLYIFFIADVLGAEDQAIYMLTFFFLVSFGSVP
                     FWVKMARRIGKHRAYLTSFFLLAFAHPFYLLLGEGDFWWMLPVTFATGFASGGFSQTL
                     PNSMKADVIDLDTLESGENRAALFFSAWSFAQKATASVGSSIALFGLALFGFDAAPEA
                     ANGPDELFGLRFLFSTFPSLFFLTGAAIVWSYPITEKRHEEIRRGIEARLQAGR"
     gene            complement(10081..>10632)
                     /gene="pgaD"
                     /locus_tag="AC16UZ_05535"
     CDS             complement(10081..>10632)
                     /gene="pgaD"
                     /locus_tag="AC16UZ_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0043708 - cell adhesion involved in biofilm
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HIR6246179.1"
                     /translation="SGPSGRARTGESSSMLPQTEPGLIIERPELQTAVQRVGGVSLTL
                     ICWAIWVYLVVPLLSLVAWVVGAHTVHSLMLQNLGVDDLLEKVGYYGAGILALVTVYM
                     TWAMVSFFRYRNMSRRSAPEIPTASVLGATHGLTHLQIQLLRSARRYVVSTEDLERMF
                     SELDEPDALEKAPADSEQDPKAA"
BASE COUNT         1812 a         3575 c         3377 g         1868 t
ORIGIN      
        1 aattgcagct gggcgccgtg tacatggttc cttcggccac ttcgttgacg tgggcaaccc
       61 actcggtttc cgattcggcg agaggctcga tgcgcgtgcg gttggtctcg cgcagccacg
      121 tgatgcactc gccgatccag ttcacgtgct gctcgatggc caccagcatg ttgcagagca
      181 ccgaagggct gcccggcccc gtaacggtga acaggttcgg aaacccgcgc atctgcagac
      241 ccaggtacgc aaccggaccg tatcgccact tgtctgccag ggcctggcca tcgcggccgc
      301 gaacgttcgc cctggtcagc gcgcccgtca tggcatcgaa gccggtggcg tagatcagca
      361 cgtcaagatc gtactgaccg tggctggtcc tgatcccgtc gggattgagc gtttccatgg
      421 gtgtctcgcg caggtcgacc agcgtgacgt cgtcgcggtt gaaggtctcg aaaaagtcgc
      481 ggtccagcac ctggcgcttg catcccagcc catagtcttt cggcagcagc ttctccgcgg
      541 tctccggatc ccgcacctcc gcccggaccg cgtcccggta aagggcgttg gcaacctcgt
      601 tggcctccgg gtccgtgtag acgtcgggga agttgagcag cgtttccaga cctcgctcgg
      661 caacgatccg acggcgttga tcggggtcga gatcgaggat gcccgtgcgc aaggcgcgct
      721 cgccgggcac cgcgggacca gcccgccctc ctctgagcgg ccgatatccg gaaaccccac
      781 cggcattgcg ctgctgcatg ccgcggatgc tgcgatagtg ctcacggaac tcgcgctgca
      841 cgtcgggccg catcgccttg cggttcgccg ggaccgtgta gacgggcgtg cgctgaaaca
      901 ccaccaggtg tcccgactga cgagccagct cgggaatggc ctgaacgccg gaggatccgg
      961 tgccgatgat gcccacccgc ttgcccgcga gatccacggc ttcgtggggc cagcgtccgg
     1021 tatggagcac gagcccggcg aaggagtcgg cacccggcag gcggggcgtg ttggggacgg
     1081 acaggcaacc ggtggccatg acgcagaacg gcgcttcgaa gacctcgccc gcctcgctgg
     1141 tcaggcgcca gacgttctca tcctcccggt actcgacggc gacgatccgc gtatcgaacc
     1201 gcatgtggcg ccggagatcg aaccggtcgg ccacgtggtt cgcgtaggcg agaatgtcct
     1261 cctgtgagga aaatacctcg ggccagtccc actcctgctc cagctccggg gaaaagccgt
     1321 aggagtattc caggctcgga acgtcgcagc gggcgccggg gtagcggttc cagtaccacg
     1381 tgccgccgac gtctccaccc gcctccagca ccttcaccgt gaggccgagc tcgcctaagc
     1441 gatgcacggc gtacatcccc gcaaaacccg cacccaccac cagcacgtca ggttcgtcgt
     1501 tcatccgggc gccggattcc cctctttgac atgacctgat tctaagggca gccgatgctc
     1561 agcgtcaccg gccggccctg cagggaactc gcagcgatat tccatgcctg tcgggttaca
     1621 ctgccgacgt cgtctggagg gagagatttc agtgctgata ctgcaccacg cgcccaattc
     1681 acgggccgga cgcatcgtct ggcttctcga ggagcttggg ctggattacg agctcaaccg
     1741 catggatttc catcccaggg acctcaagtc cgaggcccac cgcgaacgcc acccgctcgg
     1801 ccgcgtaccc gttttagagg acggggagat catgctctac gagtcgggcg ccatcgtgga
     1861 gtacatcgtc gcgcggcaca gcgacggcgc tctcaagccc tcagtggact cgccgctgtt
     1921 ccccgagtac ctgcaatggt ttcactactg cgagggcatg gtcatgccgc cggtgaacac
     1981 catcgtcgtg cagacgctgc tgctgcctcc cgagcggcgc aacgaggagg ccctcggcca
     2041 ggcccagcga ctgctggcca aggcggtggc acccgttgac gaggccctgg cgggccgcga
     2101 atacctcatc ggcgactttt ccgccgcgga catcatgctg ggtcacgccc tttacatgtc
     2161 ccggcgcctg ggtgccgtct ccgaggagat gaccaacctc aacgcctaca tcgaccgcat
     2221 cgaagcccgg cccgcgttcc agaagggcat ccaggcctga tccggcgggc ggacagggcc
     2281 cggagcggaa aatgagcggc gcgcgatcac actggtccgt cggcggccgc aactacgtgc
     2341 tggtgctgct cacggcaacc gccgccttca acttcgtcga tcgacagatc ctcggggtgc
     2401 tgctcgagcc cataggtcag gagttcgacc tgcccgacag cctgctgggc ctgctttcgg
     2461 gcatcgcctt tgccgttttc tacgcggtgc tcggcattcc catcgctgcc ctcgccgatc
     2521 gtctcaaccg ccgcaacatc atcgtggtgt gcatcgcgct cttcagcacc atgaccgctc
     2581 tgtgcggggc ggtggtgaac ttctggcagc tgttcctcgc gcgcatcggc gtgggggtgg
     2641 gcgaagccgg caccatgccg gccagccaat cgatgctctc cgatctcttt ccggtccagg
     2701 agcgaccctg ggccatggga ctgctggcca cgggaggcaa catcggtctg atgctcggcc
     2761 tgctcatcgg cggctgggtg aacgagtggt tcggctggcg ggccgccttc gtgaccgcgg
     2821 cggtacccgg cgcgctcatc gccgcagtca tctacttcac cgtcccggag cccgtccgca
     2881 ggcacgcggc ggctgcgcgg tccggctgga tgcgcgaggc cttcaccggg atgcggctgg
     2941 tgggcagtat ccgctcggtc cgccgattcg ccatgggagg cacgctctac ggtctttcgg
     3001 cctacggcat ccacacctgg atgcccgttt actacatccg ctaccacggc ctttccaccg
     3061 gcgaagcggg aacggtgatc tccatcatcg tcggcgtgct cggcggcatc ggcgctttcg
     3121 ccggcggcgt cttctgcgcg aagctcagcc agcgcgatct gcgctggaac ggctggctgc
     3181 cggccctggc gatcttcacc agcgtgccgc tcctgatcgc catgctcatc accgacaata
     3241 cctggctcgc catcggcctg ttcgtgctcc ccgggatcct ctccagcgtc tatgccgggc
     3301 ccacctggtc catcattcag gaactggtgc ccgcgcagcg gcgggccatg gcggcgtcgg
     3361 tttacatgct tttcttcaac ctcatcggcc ttggcctggg gccgctgacg gtgggggttc
     3421 tcagcgacct gtacattccc gccattggcg aggcttcgct gcgctgggcc atggtgagcg
     3481 tgctgctcgt gagcctggga ggcatcaccg cctacgtgct ggcagcgagg tccctcatcg
     3541 gcgatctgga cgtgctacgc tcgcgtgccg gaggtgccca aacatgaccg tcaatactca
     3601 ggtgctcatc gactcgctgc cgcaggggaa gctcaccgct gacaactatc ggatcgccga
     3661 gagccccgtg cccgagatcg gcgacggcga ggtgctcgtg cagaccacgg ccttcgccat
     3721 cacggccggc acccgggcgg ggctccaggg aagcgccagc tatgccggcg cgccgcagac
     3781 cggggtggtc atgaacggca ccggcgtagg cgaggtgacg gaaagccgct ctgaagactt
     3841 cgccgcgggc gatcgggtgc tcgggcccac cggctggcag gcgtactccg ttcacaaacc
     3901 gcgcgcgctg acgctcatcc ccgagggcca cgatcccgtt gactatctgg ggcctctggg
     3961 cgtgaacggg ctcaccgcct acttcgggct cttcgaggtg ggccgcccgg cagccgggga
     4021 aacggtgatg atctcggccg ccgccggcag cgtcggccac ctggtggggc agatggccac
     4081 aacggtggac gcgcgggtcg tgggcgtttg cgggagcgac gccaagggcc gcattctcac
     4141 ggatgagctc ggcttcgacg ccgtggtcaa ctaccgggaa gacggcttcc gccaggcgct
     4201 caaggaagcg acacccgacg gtgtggacgt ctacttcgac aacacgggtg gcgacatcct
     4261 cggtgctgcc ctgcggcgca tgaacgtggc cggccgcatc gcctgctgcg gcgtggtctc
     4321 ccagtacgac accgacaatc ccgaacccgg cccgcgcggg atcccggggt tgctgatcaa
     4381 caagcgcctg gacatgcgcg gctttctcgt tttcgatttt gccgaccgct acgacgaggc
     4441 gcgcgggaat attgcagcct ggctcgagtc gggcaaactg aaggccctgg tagacgaagt
     4501 ggacggggtc gaggccgcgc ccgccgcgtt cgtcgatctg ttagccgggg gcaacgtggg
     4561 cacgcgcgtg gtccgcgtga cctgaacgtc gaggaggaac ctggaatggc tgaaaccaac
     4621 attcagtcgg ctccgcagga gagccgcgca gaaaagctgg acgccggaca cccgcaattt
     4681 ctgcgcggag atcccatcac cggcgatcgc tactacagcc aggactttgc ccgccgtgag
     4741 tgggaccgaa tgtggacgcg tgtgtggcac atcgccgggc gggaatcgga gctgcccgac
     4801 gccggcgact ggatcgtcca caattttctg cgcgagtcca tcatcgcgac ccgtcagagc
     4861 gacggatcga tccgcgcgtt ctacaacagc tgccagcacc gtggtaaccg gctggtgtgg
     4921 gcaggcgagg gtcgcggcaa cttcacctgc tcgtatcacg gctgggagta cggtaccgac
     4981 ggcgtgctcg tgcacgcgca ggaccccgaa gacttcgacg gcggcagccc gtgcggccgc
     5041 gtctccctgg tcgaggtgcc ctgcgacacc tggggcgggt tcgtctggta caccatggat
     5101 ccggaggcgc cctcactcgc ggcgttttta gaccccatcc cggagctgtt cgccaatcgc
     5161 gatctcgacg ccgccgttcg cgtggtgcgg cgcgccttca ccgtggacgc caactggaaa
     5221 tttgcctccg acaacttcaa cgagtcctac cacctgccca cggtgcaccc cgagctcgcg
     5281 acgacgacgg acgaggacta ccgcaacacg atcttcgaag cctactccac cggccacaac
     5341 cggatgatcg agcagggcca gccttccatg cgcgcgccac gccccaacga ggtggaggcc
     5401 ccgtgggatg agatcctggc cgcgtgggac ctggatcccg ccgattttgc cgcgcgtgcc
     5461 agggatgggc gcatcgcgct ccagcagcaa cggcgcaagc tcggccccgc gcgcggctac
     5521 ggacactacg cgaagtactc ggacgacgag ctcaccgact attttcatca caccctgttc
     5581 cccaacgtga cgctcacggc aacggcggac ggcgtgcact tttttcgtac ggagcctcat
     5641 cccgccgatc ctgagaaatg cacgttcgaa tactggtttt tcgcatcgcc cgtcgaggga
     5701 caggacgaag tggtcacgat ctgcggcccc cgtccactgc aggaggccga gcccgaacag
     5761 ttcgaatacg gctccggtgc ggtcatcgag gacatgatgg attcgtttct gatccaggat
     5821 ctcggggtgg cggtcggcca gcagcagggg tttcattccc gcggctatcg cgacgcgatc
     5881 ttaagcggtc aggagagccg ggtgcggcgc ttccacgagg ttttaaacga ctatctggaa
     5941 ggtcgccgtt agcatccgcc agctgcgtgg aaatcggcct gcttgagatg tcgtcggagc
     6001 gctaggcgct cgcccacgcc cgcgcgcggt ctaaagcgcg tcgatagtgc ttcagaccca
     6061 gggccaggca gaggctggag atcgtgtagt tcacgacggc aagcagcgag atggacttac
     6121 ccagcgccat ctcgctgccg aacacgaaat cgctcagcac ggccggcaat gacggcgcga
     6181 acgtgtacgc gagaaagttc accgcgatga gatagaaggc caccatctgg ccgcgcagct
     6241 cgttgggagc gatggcctgc agggtcgcca tgatgaggcc cggcggcatg gccatgcaga
     6301 acgtggcggg caccagcaga gccacgccca gccaggggtc ggggacgatg gtgatggcgg
     6361 ccccgactgg caggagcacg atggatccat acatgacgac ccggatcgtg ccgtccggcg
     6421 agtcgcggcg gatcagccgc cccgcccagg ccccgccgat gatgctgccc gccatgccga
     6481 cgaacatggc gatgaacccg taggtcagtc cgatctccgt gcgcgtccac tcgtggttgc
     6541 gcacgtagaa ctccacgagc caggagaaaa gtgaccagcc ctgggtagcc aggcacaggt
     6601 aggcggtgaa gaccaggaag aagaaagccc cccgatcctt gacgaaggcc cagacctcgc
     6661 ggagcggcac gccggcctct tcagtctgct tgccgtgtcg ggacggctcg cgcagccccc
     6721 acatggcgag cgccaccagc agcccgggcg cgccaaccag gaggaacacc atctgccagg
     6781 aaaatacctc accaaagagc ggcagcacgt agttgggtgt tgcctccagg tagtcgataa
     6841 gcggcccgcc ggcgatgttg gccaggccct gcccgatgaa tggcgccgag gccacgatgc
     6901 ccatggcgag cggcagcatg tgaggtggaa agtagtcgga aagcgaggac gtggccgccg
     6961 gttgcagagt cgcctcgccg accccgaccc ccatgcgcgt ggcgaagagg tgccagtact
     7021 ggctcaccat gccgcaaagc atggtcatcg cgctccaggc ggcaatgccc acggccatca
     7081 ggttcttccg gttgtagcgg tcggcaaccc agccggccgg ataggaaagc acgctgtaga
     7141 ggaaggtgaa ggcgaagccc atgatgagcg agacctggaa atccgagatg ccgcccaggt
     7201 cgcgcttcac gggtccgatc atcacgttca ggatctgacg atcgatgatg gagaaggtgg
     7261 aggcgatggt cagcaccgcc accacgtacc aaaggtatgc ctggctgaac ctgtcccgca
     7321 tgggtgcccg gggcgggctc agcgcggagc cagagcggcg atctcgccga gactctgctc
     7381 ccgggacgtg cccgtggtgc cgaagacgta ggattgcccc gtcggtccgc gccgaccgtg
     7441 ggcaagcacg ttcagaaccg cctggcccac gtcctccggc cgcatgccgg cctcggcgat
     7501 gggcaccgaa accgcccgca gcatcggcgt gtcggtagcg cccatgcaga gcgcgttgac
     7561 ccgtatgttg tgctcggcga gctccacggc ccaggattcg gtctgccgcc acattaccca
     7621 cttcgcaccg tcgtaacctg tggcgcccgg tccggtcatg aggtgccctt ccttgatgtg
     7681 ctcggtgatg atgttgacga tgttgccgcc gccggactcg cgcatggcgg gcacggcggc
     7741 ccgggcgcag tagtagctgc ccagggcgca gatggcgatg gatcgttcgt acgcgcgggt
     7801 ctgggcatcg tagctgccca ccgtgagaga ggtgtccacg tagatggcgg cgttgttcac
     7861 cagcacgtcg attcttccca gcgcgcccag cgcctcgctc atggcacgcc gcacctgggc
     7921 tcgatcggca acatcacagt cgagcgcgag cccgcgccca cccgcctcct ccgcgagccc
     7981 ggccgtctcc tggtttccca cgccgtcgat gtccaggcaa gcgaccgccg cgccctgttc
     8041 cgccagagcc acggcgatgc cgcgcccgat tcccacggcc gcaccactga tgacggcaac
     8101 ctgacctttg agattcacgc agcctccttg ggatcagtaa ggcatgtcca gctcgatccg
     8161 ccgccgggcg atgcgccaat caccgttgtg cagcaccacc tcgtcccgat acaagccgga
     8221 ggtaaccaga cggatctggc cgtgctccac cgaagtgatg gtgatgttcg agatcagcct
     8281 ggcgcgctcg tctccgacct cctcgaaaaa cacgttcgtg accacgtggc gtcgctgatc
     8341 ggtctgctcc gccatggaat cggtcatcag ccccatgatg gcctcgcgac ccgcgaagga
     8401 catcgtgccg tcgacgccct ggatatcgat ctcgaaggca gcgtcttccg taaacgttcc
     8461 ttccagcata tccacgtgac gttcgtccag ggcgtaagcg gagcgcccca acagatcgtg
     8521 aatggcgagc ttgactgcgg tttccatctt cccctcccag cggatccggc cgtcgatgca
     8581 gccccgacgt tatggcattc cttgcaggat tgccagccgc agcagcgcca ggaatgttga
     8641 ggcgatgatc ggcccgggcg ccaccgttgc gagcttcctt gacacgcaaa tgcgccgcca
     8701 agtccaatag gccgctccgg aaggaaatcc acgtgcagcg gctgaaaaac tccacgctgt
     8761 tttactacag ccttaccgac ctgcccattg ccatgtcgat gttccccgtg gccgtgttca
     8821 tcccgcgttt ttacgccagc gacgtcggcg ttcccctggc cgtggtcggc acgatcatcc
     8881 tggtggtacg gatcttcgac gtggtcaccg accctctgat gggctacctc agcgatcaaa
     8941 cccgcacccg gttcggccgg cgcaagccct ggatcttcgc tgcgacgccc atcatgatga
     9001 cctcgatcta catgctgttc atgccccctg aagacgcggg ctggctgcac atgttcacct
     9061 ggagcatgct gctgtccatc gcgatcacga tgatgctgat cccctactac gcgtggggcg
     9121 cggagctctc accagactac aacgagcgct cgaggataac gggcggacgg gccatggccg
     9181 gcgtgttcgg cagcctgagc gcgcagctga tccccgcggg cgccctgctc ttcttcggcc
     9241 tgggcggcag cgacgtggta ctgacgctgg tgggcacgac catgctcgtg ctgatgccca
     9301 tttgcgtgct cctcaccctg ctcaataccc cggagcccga ggtgcgccac aagtcaacgg
     9361 taccggtggg caaggggctc aagctgatgt gggcaaacgg ccctttcaaa cagctggtcc
     9421 tcgccttcat gatcggctcc atcggcctga acatcactac gccgctctac atcttcttca
     9481 tcgccgacgt cctcggtgcc gaggatcagg cgatctacat gctcaccttc ttttttctcg
     9541 tcagcttcgg ctccgtgccg ttctgggtga agatggcgcg gcgcatcggc aagcacaggg
     9601 cttacctgac gtccttcttt ctcctggcct tcgcccaccc cttctatctg ctgctgggcg
     9661 agggcgactt ctggtggatg ctcccggtca ccttcgcgac cggcttcgcc agcggcggct
     9721 tctcgcagac ccttcccaac tccatgaaag ccgacgtcat cgacctcgac accctggaga
     9781 gcggagagaa ccgggcggcc ctgttcttct ccgcctggtc cttcgctcag aaggcgaccg
     9841 cctccgtggg cagctcgatt gcgttgttcg ggctcgccct gttcggattc gatgccgcgc
     9901 ccgaggcggc caacgggccc gacgagctgt tcgggctgcg ttttctgttc tcgacgtttc
     9961 cttccctgtt cttcctgacg ggcgcagcca tcgtctggag ctaccccatc acggaaaagc
    10021 gtcacgagga gatcagacgg ggcatcgaag ccaggctgca ggcgggccgg tgacgcgggt
    10081 tcaggctgcc ttggggtcct gctcggaatc agcaggcgct ttctcgagcg cgtctggctc
    10141 atccagctcg ctgaacatcc gctcgagatc ctcggtggac accacgtagc gcctggcgct
    10201 tctcaacagc tggatctgca gatgcgtcag gccgtgggtg gcgccgagca cgctggcggt
    10261 gggtatttcg ggagccgatc gcctggacat gtttcggtag cggaagaagg agaccatggc
    10321 ccacgtcatg tataccgtga ccaacgccag tatacccgca ccgtagtagc cgaccttctc
    10381 aagcaggtcg tcgacaccca ggttctgcag catcaacgag tgcaccgtgt gagcgccgac
    10441 cacccaggcc acgaggctga gcaggggcac cacaagataa acccagatgg cccagcagat
    10501 gagggtcagg ctcacgcccc ccacacgctg cacggccgtc tgcagctccg gccgctcgat
    10561 gatcagaccg ggttcagtct gcggcagcat ggaactgctc tccccggtcc gggctcgtcc
    10621 agacggcccg ct
//
LOCUS       DBNHOX010000248         2572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_727672, whole genome shotgun sequence.
ACCESSION   DBNHOX010000248 DBNHOX010000000
VERSION     DBNHOX010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_727672"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..686)
                     /gene="idi"
                     /locus_tag="AC16UZ_05540"
     CDS             complement(117..686)
                     /gene="idi"
                     /locus_tag="AC16UZ_05540"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HIR6246180.1"
                     /translation="MSHPATKSEIVSSEEELLILVDGDDREVGFLEKSACHDGAGVLH
                     RAFSLFVLNSAGEVLIQQRASGKRLWPDYWSNSCCSHPRRGETMHEAARRRLHQELGM
                     TAPLEFLYKFEYQADYRGLGAEHELCSVYVGHSDATPVINTTEIGDWRWIARDDLKRE
                     LDTRPEQFTPWFLMEWERILRDHPAALHA"
     gene            816..1703
                     /locus_tag="AC16UZ_05545"
     CDS             816..1703
                     /locus_tag="AC16UZ_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6246181.1"
                     /translation="MSAYGHPREHRFQGSQVELAWYEWGEAPHPKVLLVHATGFHARC
                     WDQVVAHLPPGFHILAVDLRGHGRSTKRGPYVWSSFLADLEEFAAALSLAGAVGVGHS
                     LGGHCITQLAARRPGAFDRLVLVDPVILDPEAYARPRYADLQSAEDHPVARRRDHWAG
                     WEEMFERFSSRKPFSRWRPQVLEDYCRHGLEPRAEGGWRLACPPVVEASIYLGNKGSD
                     VYGMLHDVDVPVAVLRAPGRDPDDHDIMDFSKSPTWPELADRFPRGRDVFLPRLDHFI
                     PMQAPELVARFITDGDADP"
     gene            1700..1954
                     /locus_tag="AC16UZ_05550"
     CDS             1700..1954
                     /locus_tag="AC16UZ_05550"
                     /inference="COORDINATES: protein motif:HMM:NF016141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR6246182.1"
                     /translation="MNFLSWLLLGLVVGTLAKLIMPGPDPGGFFVTIGIGMAGAFIGG
                     YGAHLLGIGDVAGFDLASLGLATAGAVLLLGTYRVVRGGF"
     gene            complement(1927..>2572)
                     /locus_tag="AC16UZ_05555"
     CDS             complement(1927..>2572)
                     /locus_tag="AC16UZ_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6246183.1"
                     /translation="FAEGLRAVWETALIRNIIGLNFISSLFNAGAYIIGIPYIVKEVY
                     AGDAAFLANVMIVFTIGSIGSNVLLLAFMPLKHPGRLYLLLQLTRMFILGLLWLQPSL
                     WLFYVLIVAWGLNMGVTTTLVRTTVQELAPEHHRAQILSVLLLSFVVSSPISSILLGV
                     LIAETEPLTAMLPGIAISLLIFLIGTTRSGLWGYVSPATKTRAAGPQNPPRTTR"
BASE COUNT          410 a          803 c          875 g          484 t
ORIGIN      
        1 gggcaggtag agctgctttt cacgttcgcg gcctccatct ggttcttcgg cgaacgggtc
       61 ggctggcgcg aagcggtggg cgtcgccctg gtcgtgatcg gtatctgggt gttgctctag
      121 gcgtgcaggg ccgcagggtg gtcgcgcagg attcgttccc actccatgag gaaccagggc
      181 gtgaactgct ccggccgcgt gtcgagctcc cgtttcaggt cgtcccgggc gatccagcgc
      241 cagtcgccga tctcggtggt gttgatgacg ggtgttgcgt cgctgtggcc gacgtagacg
      301 gagcagagct cgtgctcggc gcccagcccg cggtagtccg cctggtattc gaacttgtag
      361 aggaactcga gcggcgcggt catgccgagc tcctgatgca gcctgcggcg cgcggcctcg
      421 tgcatggtct ctccgcgccg tggatggctg cagcagctgt tggaccagta gtcgggccac
      481 agccgcttgc cggaggcccg ctgctggatg agcacctctc cagcggagtt gagcacgaac
      541 agggagaacg ccctgtgcag cacgccggcc ccgtcgtggc aggcggactt ttccagaaaa
      601 ccgacctcac gatcatcgcc gtccacgagg atcagcagct cctcctctga ggagacgatc
      661 tcggattttg ttgctgggtg agacatgaag accggctcgg cgtgagaaag atcagtgtag
      721 cggaattggc gacggcggtc tgcgtgtctt gtctggcctg cccgctctcc gtagcatgat
      781 cacgttgcgg ccatcggccg cgcgatcagt ttttcatgag cgcatacgga catcccaggg
      841 agcaccgttt tcagggctct caggtagagc ttgcctggta cgaatggggc gaggcgccgc
      901 atcccaaggt gctgctcgtg catgccaccg gcttccacgc ccgctgctgg gatcaggtgg
      961 tcgctcatct ccccccgggt tttcacatcc tcgccgtgga cctgcgcggc catggccgca
     1021 gcaccaaacg gggtccttac gtgtggagca gtttcctggc agacctggaa gagtttgccg
     1081 ccgcgctgag cctggcgggt gccgtcggcg tcgggcactc tctgggcggt cactgcatca
     1141 cgcagctggc ggcgcggcgt ccgggcgcat tcgaccggct ggttctggtc gaccccgtca
     1201 tcctggatcc ggaagcttac gcgcgcccgc gatatgccga cctgcagtcg gcggaggatc
     1261 accccgttgc ccggcgtcgg gatcactggg ccggctggga agagatgttc gaaaggttct
     1321 cttcgcgcaa gccgttctcg cgatggaggc cgcaggtgct ggaggactac tgccgccacg
     1381 gcctcgagcc ccgtgcggaa ggcggctggc gtctggcctg cccgcccgtg gtggaagctt
     1441 cgatttacct cggaaacaag ggcagcgacg tctacggcat gctgcacgat gtcgacgtgc
     1501 cggtagccgt gctgcgagcg cccgggcgcg accccgatga ccacgacatc atggatttct
     1561 ccaagtcgcc gacctggccc gagctggcgg acaggtttcc gcgggggcgg gacgtctttc
     1621 tgccgcggct cgatcacttc attcccatgc aggcgccgga actggtggcc cgcttcatca
     1681 ccgacggaga tgcagacccg tgaacttcct atcctggctc cttctcgggc tcgtcgttgg
     1741 caccctcgcc aagctgatca tgcctggccc cgatccgggc ggctttttcg tcaccatcgg
     1801 catcgggatg gcgggcgcgt tcatcggggg ttacggcgcc cacctgctgg ggatcggcga
     1861 cgtggccgga ttcgatctgg ctagcctcgg ccttgccacc gccggcgcgg tgctgctcct
     1921 ggggacctac cgggtcgttc gcggcgggtt ctgaggccct gcggctcgag tcttggtcgc
     1981 gggggaaacg tagccccaca gcccgctgcg cgtggtcccg atcaggaaga tgagcagaga
     2041 gatggcgatg ccgggaagca tcgcggtcag cggctcggtc tcggcgatca acacgcccag
     2101 caggatcgag ctgatgggcg aggacaccac gaagctcaag agcagcacgg agaggatctg
     2161 cgctctgtga tgttccggcg ccagctcctg caccgtggtt ctgaccaggg tggtggtgac
     2221 gcccatgttc aggccccagg ccacgatcag cacgtagaac agccacaggc tcggttgcag
     2281 ccataaaagg cccaggatga acatccgggt tagctgcagt agcaggtaga gccttcccgg
     2341 atgcttcagc ggcatgaagg ccagcaggag cacgttggag ccgatgctgc cgatggtgaa
     2401 cacgatcatc acgttcgcca ggaacgcggc gtcccccgca tagacctcct tgacgatgta
     2461 ggggataccg atgatgtacg cgccggcatt gaacaggctg gagatgaagt tcagaccgat
     2521 gatgttgcgg atcagcgcgg tctcccacac ggcccgcagg ccttccgcga ag
//
LOCUS       DBNHOX010000249         1992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1177506, whole genome shotgun sequence.
ACCESSION   DBNHOX010000249 DBNHOX010000000
VERSION     DBNHOX010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1177506"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="AC16UZ_05560"
     CDS             <1..601
                     /locus_tag="AC16UZ_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6246184.1"
                     /translation="RRAITNQELASEISPIMINGDEVRDDEQPGRAKVERIPSLRPAF
                     KPDGTITAANSSSISDGASALLIAGESALEGAEPMARIIAHATHSRHPSEFTVAPADA
                     IRQVLDRAGWKADDVDVFEINEAFAMVTMLAIQTLNLDPSRVNVYGGACAQGHPVGSS
                     GSRLIVTLAHALERLNKKRGVAALCIGGGEATAIAIERP"
     gene            749..>1992
                     /locus_tag="AC16UZ_05565"
     CDS             749..>1992
                     /locus_tag="AC16UZ_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012337.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HIR6246185.1"
                     /translation="MIRLPVIVGFGGINPAGRASFDHAYRRLVIDALDEASRARTYRS
                     LASLMNIHDDPDEVSTRAYIDAHTLIRRIELFDPNEVPVQRTARLAHPEDEPIRFVLA
                     TRQLPERIPAGWQVSSLTEQQVLVTVDNELDVLLPDTRISRVSSAGQAPTGFDPGALY
                     QSRNHPRGLQLTVFGASDALRSTGIPIEELKQRVAPDQFAVYSGSAMGQLDADGYGGM
                     LQNPLMGKRPTSKNVALGLSEMPGDFVNAYVLGSVGDTAGIIGACATFLYNMQHGIEE
                     IRSGRKRVVMVGNSEAPVVPDVIEAYRVMGALAEDEALMALDSRESEPDHRRACRPFS
                     DNCGFTVAEASIFTVLMDDELALQLGARILGSVGGVYVNADGYKKSIPGPGIGNYLTV
                     AKAMSLSRKLLGYESLLHRTYML"
BASE COUNT          361 a          698 c          629 g          304 t
ORIGIN      
        1 acgacgggcc atcacgaacc aggaactcgc cagcgagatc agcccgatca tgatcaatgg
       61 cgacgaggtg cgcgacgacg agcagcccgg ccgggccaag gtcgagcgga ttccgagcct
      121 tcggcccgcc ttcaagcccg acggcaccat caccgcggca aactccagct ctatttcaga
      181 cggcgcgtcc gcgttgctga tagccggcga gtccgccctc gaaggggccg agccaatggc
      241 ccggatcatc gcccacgcca cgcattcccg gcatccgagc gagttcaccg tcgcccccgc
      301 cgacgccatc cggcaggtgc tcgaccgcgc aggatggaag gccgacgacg tggacgtctt
      361 cgagatcaac gaggctttcg ccatggtcac gatgctggcg atccagacgc tgaacctgga
      421 cccgtcgcgc gtgaacgtct acggcggcgc gtgtgcccag ggacaccccg taggatccag
      481 cggcagccgc ctgatcgtca cgctggcgca cgcgctggag cgcctgaaca agaagcgggg
      541 cgtggccgcg ctctgcatcg gcggcggcga agcgaccgcc atcgccatcg agaggccatg
      601 acgtcacact aatggaaatg gcgcaggcgt ctccctacaa ttctccggtg atctggcgag
      661 cgcgcgtcct cccgcaagcg ggcgcgctgc gcgagaagca tcctgcatcc ggcgacccca
      721 agcgcgttgc cagcaacgga gagtatgagt gatccggctt ccggtcatcg tggggttcgg
      781 cggcatcaac ccggcaggga gggcgtcctt cgaccacgcc tatcgacgcc tggtgatcga
      841 cgctctcgac gaggcctccc gggcccgcac ctaccgcagc ctggcgagcc tgatgaacat
      901 tcacgacgac ccggacgaag tctccacccg ggcgtacatc gacgcccaca cgctcatccg
      961 caggatagag ctgttcgatc ccaacgaagt tcccgtccag cgcactgcca ggctggccca
     1021 tccggaggac gagcccatcc gtttcgttct ggcaacgcgg cagctgcccg agcgcatccc
     1081 ggcgggctgg caggtctctt ccctcaccga gcagcaggtg ctcgtgacgg tagacaacga
     1141 gctcgacgtg ctgcttcccg acactcgaat ctccagggtc agcagcgcag gccaggcacc
     1201 taccggcttc gatcccggcg ctctgtacca gtcgcgaaac catccgcgcg ggctccagct
     1261 cacggtgttc ggcgcgtcgg acgccctgcg ctccacaggc atacccatcg aggagctaaa
     1321 gcaacgggtc gcgcctgatc agtttgccgt ctactcgggc agcgccatgg ggcagctcga
     1381 cgccgacggc tacggcggca tgttgcagaa cccgctcatg ggcaaacgcc ccacgtcgaa
     1441 gaacgtcgcg ctcgggctgt cggaaatgcc cggcgacttc gtcaatgcct acgtgctcgg
     1501 gtccgtcggc gataccgcgg gcatcatcgg cgcctgcgcg accttcctgt acaacatgca
     1561 gcacgggatc gaggaaattc gctccgggcg caagcgcgtg gtgatggtgg gcaactccga
     1621 ggcgcccgtg gtgccagacg tcatcgaggc gtaccgggtc atgggcgcgc tcgccgagga
     1681 cgaagcgttg atggcgctcg acagccggga gtccgagccc gatcaccggc gcgcctgtcg
     1741 accgttctcc gacaactgcg gattcaccgt ggcggaggcg agcatcttca ccgttctgat
     1801 ggacgacgag ctggcattgc agctcggcgc ccgcatcctc ggctcggtgg ggggcgtcta
     1861 cgtcaacgcc gacgggtaca agaaatcaat cccgggcccc ggcatcggca actatctcac
     1921 ggtggccaag gccatgagcc tctcccgcaa gctgctcggc tacgagtccc tgctgcaccg
     1981 cacctacatg ct
//
LOCUS       DBNHOX010000250         2443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_599498, whole genome shotgun sequence.
ACCESSION   DBNHOX010000250 DBNHOX010000000
VERSION     DBNHOX010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_599498"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="AC16UZ_05570"
     CDS             <1..1213
                     /locus_tag="AC16UZ_05570"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6246186.1"
                     /translation="TLSTSRGDAELRLQHVPSDVHEGMLIWKVSNRSVARLPELYDEF
                     RFSPPLEYLRTTLPDVRLLGVQLFKWVWSLAVGLAVWVILFGTSRLTTRGAIRRGTRW
                     GAPLRYFLSVPVSISASLLLAGAVLRSLGLGLEAQRISEMQTGNLILIAWVFFTGINF
                     ARDLYIGKLQSQGREGTGALIRPLAAALKSVLGIIFVITWLDNAGYNIGALLAGLGIG
                     GVAVALVLQKPLEDVFAAFTLFTQQPIRIGDFGRFGELTGTIEEISLRTTRIRTLENT
                     VVAVPNAKFAAEPIENISARRRILYRPYVRLEFGTTQEQVENILQGVGKLLAGHPNVL
                     EEGRRVRLLRFGEIGIEIEGFAYIDTTVYVDYLEIAEELNLAIMNIVESAGARFAQLS
                     GDSVKQMVATG"
     gene            1345..2322
                     /locus_tag="AC16UZ_05575"
     CDS             1345..2322
                     /locus_tag="AC16UZ_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6246187.1"
                     /translation="MPYEQIQALNERMQRSIIGQEAVVERLIIGLLANGNLLVEGLPG
                     LAKTRAIKSLAKNLEADFSRIQFTPDLLPSDVTGTEVYYQQEGRSEFRFEPGPIFANI
                     VLGDEINRAPAKVQAAMLEAMEERQVTVAGTTHPMPELFMVMATQNPVEQEGTYPLPE
                     AQMDRFLMHVNIEYPPVEDEVLIMDLVRAEEGATGSDGGSGSEEARIPQQAIFDARAE
                     IAAIHLSDAIKRYMADLVYATRTPQRYSDDLDRWIDVGASPRGSLALDKCGRAHAWLN
                     RRDFVDPGDVRAIVHDCLRHRLMLSYEAKGEGVTSDKVIDVLLEKVALP"
BASE COUNT          425 a          828 c          790 g          400 t
ORIGIN      
        1 caccctgtcg accagtcgcg gcgacgccga gcttaggctg cagcacgtcc cgtcggatgt
       61 gcacgagggc atgctgatct ggaaggtatc gaatcgcagc gtggccaggc tgccggagct
      121 ctacgacgag ttccgcttct ctccgcccct ggagtacctg cgcaccacgc tgcccgacgt
      181 gcgcctgctg ggcgtgcagc tgttcaagtg ggtctggagc ctggcggtgg ggctggccgt
      241 ctgggtgatc ctgttcggca cgtcgcgcct gacgacacgg ggggccatcc ggcgagggac
      301 acgttgggga gcgcctttgc gctacttcct gtcggtcccc gtctccatat ccgcaagcct
      361 gctcctggcc ggggccgtgc tccgctctct cgggctgggg ctcgaggccc agcgcatctc
      421 ggaaatgcag accggcaatc tcatcctgat tgcctgggtc ttcttcaccg gcatcaactt
      481 cgctcgggac ctgtacatcg gcaagctgca gagccagggc cgcgagggca cgggcgcgct
      541 gatccggccg ctggccgccg cactcaaatc ggtgctgggg atcatcttcg tcatcacctg
      601 gctcgacaac gccggctaca acatcggcgc cctcctggcc ggcctcggca tcggcggcgt
      661 agccgtagca ctcgtgcttc agaaacccct ggaggacgtg ttcgcagcct tcacgctgtt
      721 cacccagcaa cccatccgga tcggcgactt cggccgcttc ggagagctca ccggcaccat
      781 cgaggagatc agcctgcgaa ccacgcggat aaggacactg gagaacaccg tggtcgccgt
      841 gcccaatgcg aagttcgccg cggagcccat cgagaacatc tcggctcgcc gccggatcct
      901 gtaccggcct tacgtccggc tggagttcgg taccacccag gagcaggtgg agaacatcct
      961 gcagggagtc gggaagttgc tcgcgggtca tccgaacgtg ctcgaggagg gccgccgggt
     1021 aaggctgctg cgatttggcg agatcggcat cgagatcgaa ggctttgcct acatcgacac
     1081 caccgtgtac gtggactatc tggagatcgc cgaggagttg aacctcgcca tcatgaacat
     1141 cgtggagagc gcgggcgccc gattcgctca gctctccggc gacagcgtca agcagatggt
     1201 ggcgaccggc tgatccgtgt gcagatgggg gtcgcagccg cgctgtcgac acgtccgcgc
     1261 cgccgtcggc atactgctgc ctgacaccac cgggatcatt cgtcgcgccg ctgacccgga
     1321 cgcggccggg aagcacgaca caccatgccc tacgaacaga ttcaagcgct caacgagcgc
     1381 atgcagcgct ccatcatcgg ccaggaagcc gtggtggagc ggctcatcat cggcctgctc
     1441 gccaacggca acctgctcgt cgagggcctt cccggcctcg cgaagacccg ggcgatcaag
     1501 tccctggcca agaacctgga ggccgacttc agccgcatcc agttcacccc ggacctgctg
     1561 ccctcggacg tgacgggcac ggaggtctac taccagcagg aggggcgcag cgagttccgg
     1621 ttcgagcccg gcccgatttt cgccaacatc gtcctgggcg acgagatcaa ccgggcgccc
     1681 gcaaaggtcc aggccgccat gctggaagcg atggaggaac gccaggtcac cgtggcgggg
     1741 acgacccatc ccatgcccga gctcttcatg gtcatggcca cccagaaccc ggtggagcag
     1801 gaaggaacct acccgctgcc agaggcgcag atggaccgct ttctgatgca cgtcaacatc
     1861 gagtacccgc cggtggagga cgaggtgctc atcatggatc tggttcgggc ggaagaaggc
     1921 gccaccggca gcgatggcgg ctccggatcg gaggaggcgc gcatccccca gcaggcgatc
     1981 ttcgacgccc gggcggagat cgcggcgatt cacctctccg acgccatcaa gcggtacatg
     2041 gcggatctgg tctacgccac ccgcaccccg cagcgctact cggacgacct cgaccggtgg
     2101 atcgacgtcg gtgcgagccc ccgggggtcc ctggcgctgg acaagtgcgg gcgcgcccac
     2161 gcgtggctga atcgtcggga tttcgtcgat cccggcgacg tccgagcgat cgttcacgac
     2221 tgcctgcgcc accggctgat gctctcctac gaagccaagg gcgagggcgt cacctcagac
     2281 aaggtgatcg acgtgctgct ggagaaggtg gccctgccct gactcgagca tgagcgtcga
     2341 tccccgcatc catgtcaccc tgcagcacct cacctccatg cagggagcgg ccggcggcat
     2401 cacgttcctg ccgcgacagg cttcggccag cgtcctcaac ggc
//
LOCUS       DBNHOX010000251         2241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_406036, whole genome shotgun sequence.
ACCESSION   DBNHOX010000251 DBNHOX010000000
VERSION     DBNHOX010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_406036"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="AC16UZ_05580"
     CDS             <1..1127
                     /locus_tag="AC16UZ_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003116511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6246188.1"
                     /translation="LTEDQLAIKDLAERFTAEQITPNAAEWDEKHIFPRETIRAAAEL
                     GFGGIYVSEASEGIGLGRIEAALIIEAMAYGCPSTSAFISIHNMAAWMIDQFGSDDVR
                     KRFVPDLVTMEKAASYCLTEPGAGSDAASLKTRAVRDGDHYVINGSKAFISGGGENEI
                     YVCMLRTGDEGHRGISCIVVEKDTPGLSFGAQERKLGWHSQPTAMVNFEDVRVPVSNR
                     VGEEGEGFKIAMAGLDGGRLNIAACSIGGAQRCLDEAIGYVKERRQFGSAIADFQNTQ
                     FMLADMATQLAAARALTHMAAQKVTENAADKTEFAAMAKLIATDSGSKIANDALQLHG
                     GYGYLMDYPVERFVRDLRVHEILEGTNQIMRMVIGRSLVS"
     gene            complement(1186..1971)
                     /locus_tag="AC16UZ_05585"
     CDS             complement(1186..1971)
                     /locus_tag="AC16UZ_05585"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246189.1"
                     /translation="MAGSKTANEIFDLSEKVAIITGGSRGLGLNMAWAFAAAGARVIV
                     ASRKQEVCDAAAQEIQAGTGQECLGAAYHAGSWQDGDRLAELVYARFGRVDVLVNNAG
                     MSPLYRSLPDVSEALYDKVLDVNLRGPFRLSALIGARMAEGDGGSIINVSSTAAVQPT
                     PTELPYAMAKAGINAFTLGLARAYAPKVRVNCIMPGPFLTDISKAWDMDAFNERAKTT
                     IPLQRGGEPDEIVGAALYFASAASSYTTGSVLKVDGGSAYPAA"
     gene            complement(2062..>2241)
                     /locus_tag="AC16UZ_05590"
     CDS             complement(2062..>2241)
                     /locus_tag="AC16UZ_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246190.1"
                     /translation="AKESINQAQNEQGLFSALRSAMTLQQLSHANNNMVHGMGVVPEG
                     ARQVRDLIKKPPLDE"
BASE COUNT          420 a          743 c          685 g          393 t
ORIGIN      
        1 acctcaccga agatcaactc gccatcaagg atctggccga acgcttcacg gcggagcaga
       61 ttacgcccaa cgcggccgag tgggacgaaa agcacatctt cccgcgggaa accatccgcg
      121 cggccgccga gctagggttc ggaggaatct acgtatcgga agcctccgaa ggcattggcc
      181 ttggacgcat cgaggccgcc ctcatcatag aagcaatggc ctacggctgt ccctccacat
      241 cggctttcat ctcgatccac aacatggccg cctggatgat cgaccagttc ggctccgacg
      301 acgtcagaaa gcgcttcgtt cccgacctgg taaccatgga gaaggcggcg agctactgcc
      361 tcaccgaacc aggagccggg tcggacgccg catcgctgaa aacgagagcc gtgcgcgatg
      421 gcgatcacta cgtcatcaac ggcagcaagg ccttcatttc aggcggtggc gagaacgaaa
      481 tttacgtgtg catgctgcgc acgggagacg agggacatcg cgggatttcc tgcatcgtcg
      541 tcgagaagga cacgcccggc ctgagcttcg gcgcccagga gcggaagctc ggctggcatt
      601 ctcagccgac ggcgatggtg aacttcgagg acgtgcgagt gcctgtttcc aaccgggtgg
      661 gcgaagaggg cgaaggcttt aagatcgcca tggccgggct ggacggcggc cggctcaaca
      721 tcgccgcctg ctcgatcggt ggcgcgcagc gctgcctcga cgaggccatt ggctacgtga
      781 aagaacgccg acagttcggc tccgccatcg ccgactttca gaacactcag ttcatgctcg
      841 ccgacatggc cacccagctg gcggcagcac gtgcgctaac ccacatggcc gctcagaagg
      901 tgacggagaa cgcggccgac aagaccgagt ttgccgcgat ggcgaagctg atcgccacgg
      961 attcgggctc caagatcgcc aacgacgccc tgcagctcca cggcgggtac ggctacctca
     1021 tggactatcc ggtcgagcgc ttcgtccgag atctccgcgt ccatgagatt ctggaaggca
     1081 ccaaccagat catgcggatg gtgatcggac gatcgctggt gagctgacgg ccgccaggcc
     1141 ccgctgcggc aatcgccagg tcccgcaacc aaggcccgac cgcactcagg ctgcagggta
     1201 tgcgctgcca ccgtccacct tgagtacgga cccggtcgtg tagctggagg cagccgaggc
     1261 gaagtacagc gcagccccga cgatctcatc gggctccccg ccgcgctgca gcggaatcgt
     1321 cgtctttgcg cgctcgttga acgcatccat gtcccacgct ttgctgatgt ccgtcagaaa
     1381 gggccccggc atgatgcagt tgacccgcac cttcggcgcg tacgcccggg cgaggccaag
     1441 cgtgaacgcg ttgatccccg ccttggccat ggcgtagggc aattcggtcg gcgtcggctg
     1501 gacagcagcg gtgctgctga cgttgatgat cgatccgccg tcgccctcgg ccatgcgcgc
     1561 gccgatcagc gccgagagcc ggaaagggcc cctgagattc acgtcgagaa ccttatcgta
     1621 aagcgcttcg ctcacgtccg gcagggagcg atacagaggc gacatgccgg cattgttgac
     1681 gaggacgtcc acccgcccga aacgggcata taccagttct gccagccggt caccgtcctg
     1741 ccaggagccc gcgtggtagg ccgctcccag gcactcctga cccgtgccgg cctggatctc
     1801 ctgcgctgcc gcgtcgcaga cctcctgctt tcggctggca acgatcaccc gggcacccgc
     1861 cgcggcgaag gcccaggcca tgttcagacc cagtccccgg cttccgccag tgatgatcgc
     1921 caccttttcc gaaaggtcga aaatctcgtt cgccgttttc gatccggcca tggtcatctt
     1981 gctcctctaa cccgtttcca ctgtgccgcc gtcactcgtc agcgccaagc tgacgggttg
     2041 ccggctcctg cgccgccgcg gtcattcatc taaaggcggt ttcttgatga gatccctcac
     2101 ctgccgggca ccctcgggca ccacgcccat accgtgcacc atgttgttgt tggcatggga
     2161 cagctgctgg agagtcatgg cgctgcgcag agcgctgaac aggccttgtt cgttctgagc
     2221 ctgattgatc gactccttgg c
//
LOCUS       DBNHOX010000252         1679 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_85046, whole genome shotgun sequence.
ACCESSION   DBNHOX010000252 DBNHOX010000000
VERSION     DBNHOX010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_85046"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="AC16UZ_05595"
     CDS             <1..719
                     /locus_tag="AC16UZ_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR6246191.1"
                     /translation="IKRQMLDWWGPILHEYYSGTEGNGQTAITPEEWLAHPGSVGKPV
                     LGNVHILDEAGNEAPIGTEGCVYFGDGARFEYYKDPAKTAASYDERGWSTLGDIGYLD
                     QEGYLYLTDRASHMIITGGVNVYPREVEDVLVTHPAVQDAAVFGIPDPEFGEQVKAAI
                     ELVPGTTVNGDELIAFCRARIAHLKCPKSVDFHDTLPRHQTGKLYKEALKRPYWPEPS
                     ANKEPSANKEPSANKEPSAE"
     gene            complement(708..>1679)
                     /locus_tag="AC16UZ_05600"
     CDS             complement(708..>1679)
                     /locus_tag="AC16UZ_05600"
                     /inference="COORDINATES: protein motif:HMM:NF025670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HIR6246192.1"
                     /translation="RDESDVWTQQAYLKASNADNGDEFGRYLDLDGDTLVVGAWHEQS
                     AGAEDDNSLADAGAAYVFVRDESGEWSQTAYLKPDEIDTGDLFGGNVALSGDTLVVGA
                     TGEDGVAGDPADNSSNNSGAAYVFERDLAGGWSQSAYLKADNADPDDAFGANPDLEGD
                     VLMVAAIGEQSALPGDPDDNSGNNVGAVYEFARDIDGNWSQTGYFKASNAELGDEFGS
                     AVAYEGDTLVVGARKEAGLSGNENDNSGARVGAAYVFFREDGIWRQAAYVKASNPDNE
                     DEFGNAVSFDGNALVVTAEGEDSAETGLGGAGLDNSLNAAGAGYLFR"
BASE COUNT          284 a          582 c          529 g          284 t
ORIGIN      
        1 ccatcaagcg gcagatgctc gactggtggg gccccatcct ccacgagtac tactcgggca
       61 cggagggcaa cgggcagacg gccatcacgc ccgaagagtg gctcgcccac ccggggtccg
      121 tgggcaagcc cgtcctcggc aacgtgcaca tcctggacga ggctggcaac gaagcgccga
      181 tcggcaccga gggatgcgtt tacttcggcg acggagcgcg tttcgagtat tacaaggatc
      241 cggcgaagac ggcggcctcc tacgacgagc gcggatggtc cactctgggt gacatcggct
      301 atctggacca ggaaggctac ctgtacctca ccgatcgcgc ctcgcacatg atcataaccg
      361 gcggggtaaa cgtctatccg agggaggtgg aggacgtgct ggtgacccat ccggcggtgc
      421 aggacgcggc cgtgttcggc attccggacc cggaattcgg cgagcaggtc aaagccgcca
      481 tcgagctcgt ccccggcacc acagtgaacg gcgacgagct catcgccttc tgccgggcgc
      541 gcatcgccca cctgaagtgc ccgaagtcgg tggacttcca tgacaccctg ccgcgccacc
      601 agacgggcaa gctctacaaa gaagccctca agcggcccta ctggccggag ccttcagcga
      661 ataaagagcc ttcagcgaat aaagagcctt cagcgaacaa agagccttca gcggaatagg
      721 tagccggcgc ccgcggcgtt cagggagttg tcgagacccg ctccgccgag cccggtctcc
      781 gcgctgtcct cgccttccgc ggtgaccacc agagcgttgc cgtcaaagct cacagcattg
      841 ccgaactcgt cctcgttatc agggttggac gctttcacgt acgcggcctg gcgccagatg
      901 ccgtcctcac ggaagaaaac gtaggcggcc cccacacgcg cccccgaatt gtcgttctcg
      961 ttgccgctca aaccggcctc cttgcgggcg cccaccacca gggtgtcgcc ctcgtaggcc
     1021 acggccgagc cgaactcgtc gccaagctcc gcgttggacg ccttgaagta gcccgtctgg
     1081 ctccagttcc cgtcgatgtc tcgagcgaat tcgtagacgg cccccacgtt gttgcccgag
     1141 ttgtcgtccg ggtccccggg cagggcgctc tgctcgccga tggccgccac catgagcacg
     1201 tcgccctcga ggtccgggtt cgcgccgaag gcgtcgtccg ggtccgcgtt gtcagccttg
     1261 aggtatgcgc tctgggacca gccccccgcc aggtcgcgtt cgaacacgta ggcggcgccg
     1321 gagttgttcg agctgttgtc ggccgggtcg ccggccaccc cgtcctcgcc ggtagccccc
     1381 accaccagcg tgtcgccgga aagcgcgacg ttgccaccga agagatcgcc cgtgtcgatc
     1441 tcgtcgggct tcaggtaagc ggtctgagac cactcgccgg actcgtcgcg gacgaacacg
     1501 taggccgcgc ctgcgtcggc cagggagttg tcgtcctcgg ctcccgcgct ctgctcgtgc
     1561 catgcgccca ccaccagggt gtcgccgtcg aggtcgaggt aacggccaaa ctcatccccg
     1621 ttgtcggcgt tgctcgcctt gaggtaggcc tgctgggtcc agacgtcgct ttcatcgcg
//
LOCUS       DBNHOX010000253         4142 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_19672, whole genome shotgun sequence.
ACCESSION   DBNHOX010000253 DBNHOX010000000
VERSION     DBNHOX010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_19672"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="AC16UZ_05605"
     CDS             complement(<1..862)
                     /locus_tag="AC16UZ_05605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR6246193.1"
                     /translation="METAKSYCRYCHAYCAIDVDVEDGRPVAIRGDAKDPVYGGYTCI
                     KGRQLPELYDAPDRLHTALKRMPDGRFEEIPTARALDEIAARIERIIERDGPRAVASY
                     CGTYAFMESAALALARSFHQGIGSESFYTSVTIDQPGRPIAMSRFGTWGGGTHSFADA
                     DVVMSIGNNAVVSSFSVFGGIPPFNPYRRMNDAIKDGKKLIVIDPRRTEVARKAHLFL
                     QVRPGEDPTLLAGIIRVILDEDLHDQPFCDQYVDGLDAIKAAVDPFTLEYVARRAAVP
                     AEQIAEAARTF"
     gene            1011..2180
                     /locus_tag="AC16UZ_05610"
     CDS             1011..2180
                     /locus_tag="AC16UZ_05610"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6246194.1"
                     /translation="MTWSPFAPEVMADPANGHRELLDRCPVHHCEDFDPPFFTLSRYA
                     DVEEALRDIETFSSHYGQGPRFTEPQGMLCDPPQHTYMRKLVQQAFTPKAMEALRIQV
                     ADLTEKLIDDILAGEPEFDLHDDFAFPLPVIIIAGMLGVPTEDLDRFKHWSDVQVAAM
                     GSSDPTRFEADQAAFFEYMVNHYRKRHAAIVAGEAVPDDLLTMIAGARAEDGSFIAEA
                     DAQSMLTQLLVGGNETTTSLITNMVWRLLEDRERWQEVVADPVLVEAAVEESLRFDPP
                     VLGLYRNTTRDVVLHDTVIPTGSKVYINYAAANRDASVFERPDEFDLHREAKRHMSFG
                     LGVHFCLGAPMARLEAEIALKALARRMPDLKLLGPGQRIAPFFLWGRRNLPVAWR"
     gene            2190..2795
                     /locus_tag="AC16UZ_05615"
     CDS             2190..2795
                     /locus_tag="AC16UZ_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246195.1"
                     /translation="MSEALSPSPRPTLAGWIIRDVLITSGLAAMLLGLAAWHEAEGSL
                     AAALVAPALGFVAAYVLCYVYHEWGHLIGARLTGAAMPLAPYAGALIGHFDIQAHSRR
                     QFLWLSWGGVIAYVALMSIALSVYLHGGLGPAGAGFAVGGLAFVVQSLSVDVPQIVRV
                     MRGDDIARTSAEGANARVILRRTWQSWLVLAGALIVWNFAS"
     gene            2838..3641
                     /locus_tag="AC16UZ_05620"
     CDS             2838..3641
                     /locus_tag="AC16UZ_05620"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013692821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246196.1"
                     /translation="MTANGLLENKIAVVVGGGQTPGETIGNGRATAIQFAREGARVLV
                     GDRDVGSAEETVAMILDEGGEAASAEIDVTVEESVASFMAAAVDRWGRIDVLHNNVGV
                     GLAGADAGVEEISADVFDQLVRINLRGMVLTAKHVLPGMRERGSGVIINISSMAAWKP
                     YPFVGYKTTKAGVIALTEQLAQANARYGIRANVILPGLMNTPMAVEPRVSQTMTREEV
                     IADRDRRVPLGRKMGTAWDVAHAAAFLASDRARFITGVALPVDGGASVS"
     gene            complement(3681..>4142)
                     /locus_tag="AC16UZ_05625"
     CDS             complement(3681..>4142)
                     /locus_tag="AC16UZ_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6246197.1"
                     /translation="PPDQELLEHIPVEMFDTAQHISSRGPFLVAKEILPGMRERGSGS
                     FLITNNAASLRGRKRYTGQSLYYPRVMMRTLAQVLTEEYSEHGVHVANIIVDGLIDSP
                     GTRALPMAQQRPEIIMNPAAIADAYYYLHTQDRSCWTHELQLTPHSTKPSF"
BASE COUNT          652 a         1336 c         1409 g          745 t
ORIGIN      
        1 cgaatgtgcg ggcggcttcg gcgatctgct cggccggtac ggccgcgcgt ctggcaacgt
       61 actccagggt gaacgggtcg acggcggcct tgatggcatc caggccgtca acgtactggt
      121 cgcagaaagg ctgatcgtgc agatcctcgt cgagaatcac gcggatgatg cccgccagca
      181 gcgtcgggtc ctcgccgggc cgaacctgca ggaacaggtg ggccttgcgg gccacctcgg
      241 tgcgccgggg gtcgatgacg atcagcttct tgccgtcctt gatggcgtcg ttcatgcgcc
      301 ggtaggggtt gaaaggcggg atgcccccga agacggaaaa cgaggaaacc acggcgttgt
      361 tgccgatgct catcaccacg tcggcatcgg cgaagctgtg ggtgccgccg ccccaggtgc
      421 cgaagcggga catggcgatg ggccggccgg gctggtcgat ggtcaccgag gtgtagaagg
      481 actccgagcc gatcccctgg tggaagctgc gcgccagcgc cagtgcggcc gactccatga
      541 aggcgtaggt tccgcagtag ctggccacgg ctctcggccc gtcgcgctcg atgatccgct
      601 cgatgcgcgc ggcgatctcg tccagggccc gggccgtggg aatctcctcg aagcgcccgt
      661 cgggcatcct cttcaaggcg gtgtgcagcc ggtcgggggc gtcgtagagc tcgggcagct
      721 ggcgcccctt gatgcacgtg tacccgccgt agaccggatc ctttgcgtcg ccgcgtatgg
      781 cgacgggccg cccgtcctcc acgtccacgt cgatggcgca gtaagcgtgg caatagcggc
      841 agtagctctt ggcggtttcc atccgcgtcc tcccccgaat tcgccgaatc aagctttgcg
      901 cacggcgagg tgcgattcaa gtcccggcga gttgccggtc gctggcgcat gcggtgtcca
      961 attaggcaaa cgccgatata ctggcgagcc cgcacttcag gaaggtcgat atgacctgga
     1021 gtcccttcgc gcccgaggtc atggccgacc cggccaatgg ccatcgggag cttctcgacc
     1081 gatgccccgt ccatcattgc gaggattttg atcccccgtt cttcacgctt tcccgctatg
     1141 ccgacgtgga ggaagcgctg cgggacatcg agacgttttc cagccactac ggccagggcc
     1201 cgcggttcac ggaacctcag ggcatgctct gcgatccgcc tcagcacacc tacatgcgca
     1261 agctggtcca gcaggccttc acgcccaagg ccatggaagc gcttcgtatt caggtcgcag
     1321 atctcaccga aaagctcatc gacgacatcc ttgccgggga gccggaattc gacctgcacg
     1381 atgatttcgc cttccccctg ccggtgatca tcatcgccgg catgctgggg gtgcccaccg
     1441 aagacctcga tcggttcaag cactggtccg acgtccaggt agcggccatg ggatcgtcgg
     1501 atcccacgcg ttttgaggcg gatcaggccg ccttcttcga gtacatggtc aaccactatc
     1561 gcaagcgcca cgcggccatc gtggccgggg aagcggtacc ggatgatctg ctgacgatga
     1621 ttgccggcgc tcgcgccgag gacggctcgt tcatcgcgga ggcggatgcg caatccatgc
     1681 tcacccagct cctcgtgggc gggaacgaaa ccaccacgtc gctcatcacc aacatggtct
     1741 ggcggctcct cgaggaccgg gagcgctggc aggaagtggt ggcagacccc gtcctggtcg
     1801 aggccgccgt ggaggagagt ctgcgtttcg acccgcccgt gctcggcctc tatcgcaaca
     1861 ccacccgtga tgtcgtgctt cacgacaccg tgataccgac aggcagcaag gtctacatca
     1921 actacgccgc cgccaaccgc gatgcctctg tcttcgagcg tccggacgag ttcgacctgc
     1981 atcgggaagc caagcggcac atgtcgttcg ggctcggcgt gcacttctgc ctcggcgccc
     2041 ccatggcgag gctcgaagcg gagatcgcgc tcaaggcgct ggcgcggcgc atgccggatc
     2101 tgaagctcct cgggccgggt caacgcatag cgccattctt cctgtggggc cggcgcaacc
     2161 tgccggtggc ctggcgttag tccggcgcgg tgtccgaagc cctctcgcca tcgccgcggc
     2221 cgacgcttgc cggctggata attcgcgacg tcctcatcac ctcagggctc gccgcgatgc
     2281 tgctggggtt ggcggcctgg cacgaggcgg aaggttcgct ggctgctgcg ctcgtcgctc
     2341 cggccctcgg gttcgtagcg gcttacgtgc tctgctacgt ctatcacgag tgggggcacc
     2401 tgatcggcgc ccggctgacc ggcgctgcca tgccgctggc gccctacgcc ggcgccctca
     2461 tcggccactt cgacatccag gcgcacagcc gccgccagtt cctctggctg agctggggcg
     2521 gggtgatcgc ctatgttgcc ctgatgagca ttgccctctc cgtctatctg catggtgggc
     2581 tcggccccgc gggcgcgggt tttgccgtgg gcgggctcgc cttcgtcgtg caatcccttt
     2641 ccgtggacgt gccgcagatc gtcagggtga tgcggggaga cgacattgcg aggacgtcgg
     2701 ccgaaggcgc caacgcccgc gtcatcctgc gccggacctg gcagagctgg ctcgttctgg
     2761 cgggagcgct gatcgtctgg aacttcgcaa gctgagctga agacgacctc gcccgcgccg
     2821 ctacctggag aactgacatg acggccaacg ggctgctgga aaacaagatc gcggtggttg
     2881 tgggcggtgg ccagacgccg ggggagacca tcggcaacgg ccgcgccacg gcaatccagt
     2941 tcgctcggga gggcgcgcgg gtgctggtgg gcgaccgcga cgtgggctcg gcggaggaaa
     3001 ccgttgccat gatcctcgat gaaggcggcg aggccgcgtc cgccgaaatt gacgtgacgg
     3061 tggaagagag cgtcgcctcg ttcatggctg ccgccgtcga ccgttgggga cgcatcgacg
     3121 tgctccacaa caacgtcggc gtcgggcttg ccggggccga tgccggcgtg gaagagatct
     3181 cggcggacgt gttcgatcag ctggtgcgca tcaacctgcg cggcatggtg ctcaccgcca
     3241 agcacgttct gcccggcatg cgcgagcggg gctcaggagt gatcatcaac atctcttcca
     3301 tggccgcgtg gaagccgtat ccctttgtgg gttacaagac cacgaaggcc ggtgtcatcg
     3361 ccctaaccga gcagctcgcc caggccaacg cccgctatgg cattcgagcc aacgtgatcc
     3421 tgcctggcct gatgaacacg cccatggccg tggagccccg cgtctcccag accatgaccc
     3481 gggaggaggt catcgccgac cgtgaccggc gtgtgccgct ggggcgcaag atgggaacgg
     3541 cctgggacgt ggcccacgcg gcagcctttc ttgcctccga ccgcgcccgg ttcattacgg
     3601 gggtcgctct ccccgtggac ggcggcgcca gcgtcagctg aggctttggc tgggcgggcc
     3661 gcttgtcctg caggcgaggc tcagaagctt ggcttggtcg agtgcggcgt cagctgcagc
     3721 tcgtgggtcc agcatgatct gtcctgggtg tgcaggtagt agtaggcgtc ggcgatggcg
     3781 gccgggttca tgatgatctc cgggcgctgc tgcgccatgg gcagagcccg tgtgcccggc
     3841 gagtcgatga gcccgtcgac gatgatgttg gccacgtgga cgccatgttc cgagtactcc
     3901 tcggtcaaca cctgggccag cgtgcgcatc atcaccctcg ggtagtacag cgactgcccc
     3961 gtgtagcgct tacgaccccg cagtgaggcc gcgttgttgg tgatgaggaa cgatccggag
     4021 ccgcgttcgc gcatgcccgg caggatctcc ttggcgacga gaaaggggcc gcggctggag
     4081 atgtgctggg cggtgtcgaa catctccacg ggaatgtgtt ccaggagttc ctgatcgggc
     4141 gg
//
LOCUS       DBNHOX010000254         7680 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_728003, whole genome shotgun sequence.
ACCESSION   DBNHOX010000254 DBNHOX010000000
VERSION     DBNHOX010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_728003"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="AC16UZ_05630"
     CDS             <1..1163
                     /locus_tag="AC16UZ_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6246198.1"
                     /translation="GDTGYITFDQWIAGQPDDDPALPAAPGDTCYQLYTSGTTGLPKG
                     VELTHANFIHQVGSALAPLGIRSESVSLVCMPLFHIAGSGFGVLGMYWGADMVLLRDV
                     DLAAIVRVVPEQRVTHAMFVPAVLQFLLAQPGVRDADLSSFETILYGASPITEAVLVE
                     AMDVFGCDFIQGYGLTETTGGVSLLAAEDHDPGGPRAHLLRSCGKVIVNHEVRIVDPE
                     SRREMPDGEVGEIWVRGPQVMKGYWKNPEASAESLVEDGWFRTGDAGYLLDGYVFIHD
                     RVKDMIISGGENIYPAEIENVLMAHEGIADAAVIGVPSERWGETVKALVTRSDADLTE
                     QQIIEYCRAHLAGYKCPTSVDWIAEIPRNPSGKILKVELRKPYWEDRERFVS"
     gene            1358..3403
                     /gene="fusA"
                     /locus_tag="AC16UZ_05635"
     CDS             1358..3403
                     /gene="fusA"
                     /locus_tag="AC16UZ_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR6246199.1"
                     /translation="MAYTTADIRNVCLLGPAEAGKTQLAEALLYAGGAIAQCGQVAKG
                     NTVTDHSERERAIGHSLYPAVCHTDHAGIHVNVVDTPGYRDLYGRALAVLPAVETAVI
                     VINAEKGIEEMTRRMMEAAQAQHLCRMIVVNKIDSAEADLAGLVGEIQEAFGGECLPI
                     NLPASQGDGVVDCYFHPSGGETVFGSVEQAHTRLIDQVVEVDDELMELYLEQGDDLAP
                     EQLHEPFERALREGHLIPVCFVSARTGVGVADLLNVFERLMPNPEEGNPPEFLKGEGA
                     EAQPVSVVPDPEQHVVAHVVQVDVDPFKGRLAVFRIHQGSIRPGMQLYVGDARKPIKV
                     NQLLKLNGNGHSKVEVGLPGDICAIPRVDEAHYDAVLHDSHDEDHFHLRSEPLPPPMF
                     GLAIQPGNDADAQKTSDALHIMEAEDPSLQIEHVAALNEIVLRGLGELHLRSVLEEIE
                     ARHDLRVETALPSIAYRETITKPAQGHCRHKKQTGGAGQFGEVFLRIEPLPPGGGFEF
                     VSEVVGGAIPSQFIPAVETGVRLVLDGGAVAGYPLQDVKVTVYDGKHHSVDSKEIAFV
                     QAGRKAFVDAIRSASPIVMEPVVHATFTVPEDCVGGVSGDLAGMRGSVRGTSVLADGR
                     IEISGEAPLKDLQAYHSRLKSLTGGVGSFDMAFSRYAQVTPQTAQELMGAFAGSDEE"
     gene            complement(3456..4796)
                     /locus_tag="AC16UZ_05640"
     CDS             complement(3456..4796)
                     /locus_tag="AC16UZ_05640"
                     /inference="COORDINATES: protein motif:HMM:NF016150.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HIR6246200.1"
                     /translation="MSEVTTSVTITTVISGPVRRSDRIDSLDVMRGVALLGILLMNII
                     AFGLPFATYYNPSVAGIDNPANLWAYIVNWLFFEGTMRALFSMLFGAGMVLMTTRAEE
                     RGAGIEAADVYYRRLLWLILFGVIDAYLLLWVGDILFLYGIAGLFLFVFRGLAPRTLF
                     LIGVAMLVVLGIKNVNASNELAELRQVTAEVESIRAAGGEPSREQLAAIDHLEEIRTE
                     FEPDPEQIQEQVEARHSGYIDQVKYLGPLNIAAQSTLTYTLGIWDVLMMMFMGMALIK
                     WGVFSAARSWGFYGAMVVVGYGIGLPINWYEVRMAIESGFAIEWSGIQYVSTYQIGRA
                     GTAAGHIGLIMMICKSGLFGFITKPLAAVGRMALTNYLAHSVICMFLFTGVGLAWFGE
                     LERYELYYVVGGIWLFQLIVSPIWLRYFRFGPMEWLWRSLTYAQTQPMRRSPPG"
     gene            complement(4889..5272)
                     /locus_tag="AC16UZ_05645"
     CDS             complement(4889..5272)
                     /locus_tag="AC16UZ_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HIR6246201.1"
                     /translation="MLELNPESVAQIAEKAREFQSEEILLPPEPPPVPVDDTAFEYAA
                     EWHGDPGYNDLKDAIEDLEPDQQSVLVALMWLGRGDYGIEEWNEALAYAEEVHTDATA
                     DYLIATPLLADYLLGGLEMHGYEPE"
     gene            5405..6544
                     /locus_tag="AC16UZ_05650"
     CDS             5405..6544
                     /locus_tag="AC16UZ_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6246202.1"
                     /translation="MTENLRGLRLTSTVKSEGVLELALVESEVRSPGPDEVVVRIEAA
                     PINPSDLGLLFGLADMTTAEFSGSPERPVVQARIPGNFMKHMAGRLDEPMAVGNEGAG
                     VVVAAGASEAAQALMGRTVAVLGGAMYAQYRVLNVSMCLPLGEDTSPREGAACFVNPL
                     TALGMVETMRMEGHSALVHTAAASNLGQMLQKICSADGVQLVNIVRKPEQVEILRGIG
                     AAYVVNSAAENFKDDLTEALATTGATLGFDAIGGGRLASDILACMEAAANRAATEYSR
                     YGSTTHKQVYIYGGLDLGPTQLHRSFGMYWGVGGWLLTPFLQKIGPEATQRLKERVAD
                     EVKTTFASHYAEEVSLAGALEPEAISAYGQKATGQKYLINPNAGL"
     gene            complement(6563..7009)
                     /locus_tag="AC16UZ_05655"
     CDS             complement(6563..7009)
                     /locus_tag="AC16UZ_05655"
                     /inference="COORDINATES: protein motif:HMM:NF025050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6246203.1"
                     /translation="MTMPDNTSITQMGYVVADLDAAMERWKTTVGAGPFERLNHVEVV
                     DGLYRGTPTNVDISVAIAEVGGIQLELIEQHNDVPSCYRDLFAAGEEGLHHVAVQPED
                     FDAEVARYEALGCPQAFGGLYEGARFVYMDTSGSLGIMVELVERRE"
     gene            7066..>7680
                     /locus_tag="AC16UZ_05660"
     CDS             7066..>7680
                     /locus_tag="AC16UZ_05660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6246204.1"
                     /translation="MTAGSGTLHSDMAWPRQGYAWYVVIILFICSIFSFLDRQVIALM
                     VDDIKLDLGLNDFEIGLLQGPPFGIFYALMSIPIALAADRYSRRNIIIAGVAFWSAAT
                     AACGLAGNFWHLFLARVGVGSGEACLSPCAYSMISDYFRRQLLPLAMSIFTMGNLTGV
                     GLAMIIGGAVIGYAKSLDEIVIWGLGDVAPWQFAFIVIGLPGLLL"
BASE COUNT         1338 a         2446 c         2561 g         1335 t
ORIGIN      
        1 ccggcgatac cggttacatc acgttcgatc agtggatcgc cggtcagcca gatgatgacc
       61 ccgcgctgcc tgccgctccc ggggacacct gctaccagct ctacacctcg ggaaccacgg
      121 ggctgcccaa gggcgtggag ctgacccatg ccaacttcat tcaccaggtc ggcagcgccc
      181 ttgcgcccct gggcattcga tccgagtccg tcagcctggt ctgcatgccc ttgttccaca
      241 tcgccggcag cgggttcggc gtgctgggca tgtactgggg agccgacatg gtgctcctgc
      301 gggacgtcga tctggccgcc atcgttcggg tggtccccga gcaacgtgtt acccacgcga
      361 tgtttgtccc cgcggtgctg cagttcctgc tcgcccaacc cggggtacgc gatgctgatc
      421 tgtcgtcgtt cgaaaccatt ctgtacggcg cttcgcccat cacggaagcc gtgctggtgg
      481 aggccatgga cgtgttcggc tgcgatttca tccagggcta cggcctgacc gaaaccaccg
      541 gcggcgtgag cctgcttgct gctgaagacc acgatccggg tggcccgagg gcgcatctgc
      601 tgcgctcctg cggaaaggtc atcgtcaatc acgaggtacg catcgtcgat cccgagagca
      661 gaagggagat gcccgatggc gaggtgggcg agatctgggt gcgcggcccc caggtgatga
      721 agggctactg gaagaacccc gaggcgagcg ccgagagtct ggtcgaggac ggttggttca
      781 ggacggggga tgccggctat ctgctcgacg ggtacgtctt cattcatgac cgcgtgaagg
      841 acatgatcat ctcgggcggt gagaacatct accctgcgga gatcgagaac gtgctgatgg
      901 cccacgaggg gattgccgat gcggccgtga tcggcgtgcc cagcgagcgg tggggagaga
      961 cggtcaaagc gctggtgacg cgcagcgatg ccgacctcac cgagcagcag atcatcgagt
     1021 actgccgtgc ccatctggcg gggtacaaat gcccgacttc cgtggactgg atcgccgaga
     1081 ttccccgcaa cccgtcgggc aagatcctca aggtcgagct tcgcaagccg tactgggaag
     1141 accgggaacg tttcgtttct taagcccgcg cccaggtttc ctcgggcccg gccccagcgt
     1201 gaggcgggcg cgcaccgatt cggtgcgtgc gggcggcgtt tccacctggg tggcgtgctg
     1261 gggcgctccg tcaaccccct gttttctggc gggtgtgacc cgaccgtttc cgtgaggtaa
     1321 gcttcgaagg tgagcttgag gtagggggga agaccacatg gcttacacta ctgcggacat
     1381 ccgcaacgtc tgcctgcttg gaccggccga ggccggaaag acccagttgg ccgaagcgct
     1441 gctgtatgca gggggcgcca tcgcccaatg cgggcaggtc gccaagggca acaccgttac
     1501 cgaccacagc gaacgcgaac gcgccatcgg ccactctctg tatcccgcgg tgtgtcatac
     1561 ggatcacgcc ggcattcacg tgaacgtcgt ggacacgccc ggctaccggg acctttacgg
     1621 ccgagccctg gcggtgctcc cggcagtgga gaccgccgtc atcgtcatca acgcggagaa
     1681 gggcatcgag gagatgaccc ggcgcatgat ggaggcggca caagcccagc atctgtgccg
     1741 catgatcgtc gtcaacaaga tcgacagcgc cgaggccgat cttgccgggc tcgtcggcga
     1801 gattcaggag gctttcggtg gcgagtgcct gcctatcaac ctgcccgcct cccaaggcga
     1861 cggtgtggtg gactgctatt tccatccctc cggcggggaa accgtgttcg gaagcgtgga
     1921 acaagcgcac acccggctga tcgatcaggt ggtggaggtg gacgacgagc tcatggagct
     1981 ctacctggag cagggcgacg acctcgcgcc ggagcagctg catgagccct tcgagcgggc
     2041 gctgcgggaa ggtcacctga ttcccgtgtg cttcgtctcc gcccgcacgg gcgtgggcgt
     2101 cgccgatctg ctgaacgtct tcgagcggct catgccgaat ccggaagagg gtaacccgcc
     2161 cgagttcctg aagggcgagg gtgccgaggc ccagcccgtc agcgtggttc ccgatcccga
     2221 gcagcacgtg gtggcccacg tggtccaggt ggacgtcgac cccttcaagg gccgcctggc
     2281 ggtgttccgg atccatcagg gcagcatccg cccgggcatg cagctctacg tcggcgacgc
     2341 ccgcaagccc atcaaggtca accagctctt gaagctcaac ggcaacggcc actccaaggt
     2401 ggaggtgggt ctgccggggg atatctgcgc catcccgcga gtcgacgaag cacactacga
     2461 cgccgttctg cacgactccc acgacgagga ccactttcac ctgcgctccg agccgctgcc
     2521 gccgcccatg ttcggtcttg ccatccagcc cgggaacgac gccgacgccc agaaaacgtc
     2581 ggacgccctg cacatcatgg aagcggagga tccttcgctg cagatcgagc acgtcgccgc
     2641 gttgaacgag atcgtgctgc gcgggctggg cgagctgcat ctgcgctcgg tgctcgaaga
     2701 gatcgaagcg cgtcacgacc tcagggtcga aacggcgctg ccctccatcg cctaccggga
     2761 gaccatcacg aaaccggcgc aaggccattg ccggcacaaa aagcagacgg gtggcgcggg
     2821 ccagttcggc gaggtgtttc tgcggatcga gccgctgccg ccgggcggag gtttcgagtt
     2881 cgtcagcgag gtcgtcggcg gagccatccc gtcacagttc attcccgccg tggaaaccgg
     2941 cgtgcggctg gttctcgacg ggggtgccgt cgcgggctat cccctgcagg acgtcaaggt
     3001 gaccgtctac gacggcaagc accactcggt ggactccaag gagatcgcct tcgtgcaggc
     3061 gggcagaaaa gcgttcgtcg acgcgattcg cagcgcgtcg cccatcgtca tggagcccgt
     3121 cgtccatgcc accttcacgg tgccggaaga ttgcgtgggc ggtgtctcgg gagatctggc
     3181 cggcatgcgc ggctccgtgc gcggaaccag cgtcctggcg gacgggcgga tcgagatttc
     3241 cggcgaggcg ccgctgaagg acctgcaggc ctatcattcc aggctcaagt cgctcacggg
     3301 gggcgtgggc agcttcgaca tggcgttcag ccgctatgcc caggtgacgc cgcagacggc
     3361 ccaggagctg atgggcgcgt ttgcgggcag cgacgaggag tagccggcgg gcaacgtcga
     3421 gggcgccgcg gcccgcagcc cggggagagg ccgccttacc cggggggtga gcggcgcatg
     3481 ggctgggtct gcgcgtaggt cagcgaccgc cagagccact ccatggggcc gaagcggaag
     3541 tacctcagcc agatcgggct gacgatgagc tggaacagcc agatcccgcc caccacgtag
     3601 tagagctcgt agcgctcgag ctcgccgaac catgccaggc cgaccccggt gaacagaaac
     3661 atgcagatca cgctgtgtgc cagatagttg gtcagcgcca tgcgcccgac ggcggccagg
     3721 ggcttcgtga tgaagccgaa caggccggac ttgcagatca tcatgatcag accgatgtgc
     3781 ccggcggcgg tccccgcccg gcctatctga taggtggaga cgtactgaat ccccgaccac
     3841 tcgatggcga agcctgactc gatggccatg cggacttcgt accagttgat gggcaggccg
     3901 atcccgtagc cgaccaccac catggccccg taaaagcccc agctgcgcgc cgcggaaaat
     3961 acgccccact tgatcagcgc catgcccatg aacatcatca tcagcacgtc ccagatgccg
     4021 agcgtgtagg tcagcgtgga ctgcgcggcg atgttcaggg gaccgaggta tttcacctgg
     4081 tcgatgtagc cggagtgtct cgcctccacc tgctcctgga tctgctcggg gtccggctcg
     4141 aactccgtcc ggatctcctc taagtggtca atggcggcca gctgctcccg gcttggctcg
     4201 ccgccggctg ctcggatcga ctcgacctcc gccgtcacct ggcgaagctc agcgagctcg
     4261 ttgctggcgt tgacgttctt gatgccgagc acgacgagca tcgccacgcc gatgaggaac
     4321 agggtgcggg gcgcaaggcc gcggaagacg aagagaaaga ggccggcgat gccgtacagg
     4381 aagagaatgt cgccgaccca aagaagcagg taggcgtcga tgacgccgaa gaggatcagc
     4441 cacagcaggc gccgatagta cacgtcggcc gcttcgatgc cggcgccgcg ctcctcggcc
     4501 cgggtggtca tcagcaccat ccccgcgccg aacagcatgg agaacagcgc gcgcatcgtg
     4561 ccttcgaaga acagccagtt gacgatgtag gcccagaggt tggcggggtt gtcgatcccg
     4621 gcaacgctgg ggttgtagta ggtggcgaac ggcaatccga acgcgatgat gttcatgagc
     4681 aggatgccca gaagcgccac gccgcgcatg acgtccagcg agtcgatcct gtcgctgcgc
     4741 cgaaccgggc cggaaatcac ggtcgttatc gtgacggagg tggtgacctc gctcatgccc
     4801 ctctcctgaa acctcccgca gcctggagtt tcggcggatt gaacggcttt ggcaaccggg
     4861 tgaacccggc cgtcgtggga gcgccgcgct attcgggctc gtagccgtgc atctccaggc
     4921 ctcccagcag gtagtcggcc agaagagggg ttgcgatcag gtagtcggcg gtcgcgtcgg
     4981 tgtgcacctc ctcggcgtag gcgagcgctt cgttccactc ctcgatgccg tagtcgcccc
     5041 ggccgagcca catcagcgcc accagcaccg actgctggtc gggttcgaga tcctcgatgg
     5101 cgtccttcag atcgttgtag cccggatcgc cgtgccactc ggccgcatac tcgaaagccg
     5161 tgtcgtccac gggaacgggg ggcggctccg ggggcagcag gatctcctcg gattgaaact
     5221 cccgcgcttt ctcagcgatc tgcgccacgc tctcgggatt gagttcgagc ataccgcctg
     5281 cctcctcgtt tcattctgtc accaaccagc gcttattctt gccgttctac ggagcaagca
     5341 aatcaggggt tgtacatagg ccataacggt gtacggtctc cgggcgaaga aaggaggacg
     5401 catcatgacg gagaatctgc gaggcctgag gcttacctcg acggtaaaga gtgagggcgt
     5461 gctcgagctg gccctggttg aatcagaggt gcgaagcccc ggccctgacg aggtggtggt
     5521 acgcatagag gcagcgccca tcaacccgtc cgaccttggc ctgctcttcg gcctggcgga
     5581 catgaccacg gcggaattct cgggctcgcc ggagcgtccg gtggtgcagg cacggatccc
     5641 cgggaatttc atgaagcaca tggccggtcg gctggacgag cccatggcgg ttggcaatga
     5701 gggtgcgggc gtcgtggtgg cggcgggcgc ctccgaggct gctcaagcgc tcatgggccg
     5761 gaccgtggcc gtgctggggg gcgccatgta tgcccagtat cgggtgctga acgtgtccat
     5821 gtgcctgccc cttggcgagg acacttcccc gcgtgagggg gccgcctgct tcgtgaaccc
     5881 gctgacggct ctggggatgg tggagaccat gcgcatggag ggtcactcag ctctcgttca
     5941 tacggccgcg gcctccaacc tcggccagat gctgcagaag atctgctcag ccgacggcgt
     6001 gcaactggtc aacatcgtcc gcaagcccga gcaggtggag atcctgcggg ggattggtgc
     6061 cgcttacgtg gtgaactccg cggccgagaa cttcaaggac gatctcaccg aggcgctggc
     6121 gaccacgggg gcgaccctgg gattcgatgc catcggcggg ggccggctgg ccagcgacat
     6181 actcgcctgc atggaagccg ccgcgaaccg ggcggccact gagtacagtc gctacggatc
     6241 gacgacccac aagcaggtct acatctacgg cggtctcgat ctcggcccca cgcagctgca
     6301 tcgctccttc gggatgtact ggggcgtggg gggctggctc ctcacgccgt tcctgcagaa
     6361 gatcggtccc gaggcgacgc agcggctgaa ggagcgggtg gcggatgagg tgaagaccac
     6421 gttcgcgagc cactatgccg aggaggtctc gctggccggc gccctggaac ccgaagcgat
     6481 ctccgcctac gggcagaaag ccacgggcca gaaatatctg atcaatccca acgccgggct
     6541 ctgaaccacc ccggctggcg cgtcactccc gtctctccac gagctcgacc atgatgccca
     6601 gcgagccgga cgtgtccatg tagacgaagc gggcgccttc gtagaggccg ccgaaggcct
     6661 gggggcagcc gagcgcctcg tagcgcgcca cttccgcgtc gaaatcctcg ggctgcacgg
     6721 ccacgtggtg caggccctcc tcgcccgcgg caaagagatc ccggtagcag gagggcacgt
     6781 cgttgtgctg ctcgatgagc tcgagctgga tgccgcccac ctcggcgatg gccacggaga
     6841 tgtccacgtt cgtgggcgtt ccccggtaca ggccgtcgac gacctcgacg tgattcaggc
     6901 gctcgaacgg gcctgcgccg acggtggtct tccagcgctc catggcagcg tcgagatcgg
     6961 cgaccacgta gcccatctgg gtaatgctgg tgttatctgg catagtcatt ggctgtcccc
     7021 tgcgcagcga gagttaagga taaccttgtc cggaccccca tcaccatgac cgccggctcc
     7081 ggaacgctgc actccgacat ggcctggccg cggcagggct acgcctggta cgtggtcatc
     7141 atcctgttca tctgctcgat attctccttc ctcgatcggc aggtcatcgc gctgatggtg
     7201 gacgacatca agctggacct gggcttaaac gatttcgaga tcgggttgct gcaggggccg
     7261 ccgttcggca ttttctatgc gctgatgtcc attcccattg cgctggccgc cgaccgctac
     7321 agccggcgta acatcatcat cgccggcgtg gcgttctgga gcgcggccac cgccgcctgc
     7381 ggccttgccg gcaacttctg gcatctgttt ctcgcccggg tcggcgtggg cagcggagaa
     7441 gcgtgcctgt caccgtgcgc gtactccatg atcagcgact atttccggcg tcagcttctg
     7501 cccctggcca tgagcatctt caccatgggc aacctcaccg gcgtcgggct cgccatgatc
     7561 atcggcgggg cggtgatcgg ctatgccaag agcctggacg agatcgtgat ctggggtctc
     7621 ggcgacgtgg caccctggca gtttgccttc atcgtcatcg gcctgccggg gctgctgctc
//
LOCUS       DBNHOX010000255         6751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_855930, whole genome shotgun sequence.
ACCESSION   DBNHOX010000255 DBNHOX010000000
VERSION     DBNHOX010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_855930"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="AC16UZ_05665"
     CDS             <1..709
                     /locus_tag="AC16UZ_05665"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6246205.1"
                     /translation="QDATALAAREVFGDVLWQGYGQTEVVPVSMMGPRQWFAEVEGSS
                     PLRACGLPLPFAEVQIWDEDNQPVAAGEVGEIVARADGQMTGFWNNPEASAERIVDGW
                     VKTGDLGRLDANGYLYVVDRADDMIISGGYNIWPAELENVLASHPAVIEVAAFGVPSQ
                     KWGESPYAVCVVGNAEAVGEAELIDLVAEELGSYKKPVGVTITTDPLPKSPVGKIKRK
                     DLREPFWKGHERRVAGS"
     gene            complement(728..1144)
                     /gene="arfB"
                     /locus_tag="AC16UZ_05670"
     CDS             complement(728..1144)
                     /gene="arfB"
                     /locus_tag="AC16UZ_05670"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HIR6246206.1"
                     /translation="MSVSRIRQIPPEACSERFVRSPGAGGQNVNKVATTVQLRVDLDR
                     ANLPPAVRERLEKLAGRQVNQRGELVITASRFRTQARNRADAMSRLSELLEQAQRVPR
                     RRVATRTPAGERVRRKDTKRKRGTTKRLRARPPMDD"
     gene            complement(1193..2329)
                     /gene="epmB"
                     /locus_tag="AC16UZ_05675"
     CDS             complement(1193..2329)
                     /gene="epmB"
                     /locus_tag="AC16UZ_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HIR6246207.1"
                     /translation="MSRRPTRDRDRDRLRYETADDCAILVPDVNPMTQSAPEQSAARL
                     RTRTVADDPATGSWQEALRNAIRTPRELIEALELDPAWAPVMDGHDDDFPMLVPRSFA
                     ARMRKGDPHDPLLRQVLPDGAERITAPGFEADPLQEQRFARAGVVRKYSGRALLIASG
                     ACPVHCRYCFRRNFPYADQLASRQRWTGAVDALSADTQVTEVILSGGDPLTLTNEAIG
                     ELIRAMENVASVQTLRFHTRFPIIIPERVDAGLVDTLAATRLRTVFVVHCNHANEIDA
                     SVRRALADLKAAGAQLLNQSVLLKGVNDNEDDLAELSQRLFDAGVLPYYLHLLDPVAG
                     AAHFDVAPARGRALVAGLRRRLPGYLVPRLAREVPGESSKTILA"
     gene            complement(2326..3630)
                     /locus_tag="AC16UZ_05680"
     CDS             complement(2326..3630)
                     /locus_tag="AC16UZ_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6246208.1"
                     /translation="MKFSARTATAVIMANMIGTGVFTSLGFQLADIRSEFVLLMLWAV
                     GGLTALCGALSYAELGAALPRSGGEYNFLGRIYHPAVGFVSGWISATIGFAAPTALAA
                     ITFGIYLASVFPALSPVWLACGLVVVLSVAHATSRRSSGGVQRAFTAVKIILILGFCL
                     LAALLVDEPQGISLLPHEGDGALLTSGAFAVALIYVNYAYTGWNAATYLTGELDRPEQ
                     TLSRVLLQGTVVVMLLYVLLNATFLYVAPAEAMEGRLEIGYIAAEHAFGSNGAAIMAV
                     ILALLLISTASAMIMAGPRVLQVIGQDYPAFRFLARVSGDGVPVTAIVVQSVLALAFI
                     LTASFEAILVFAGFTLGVSTFFTVLGLFVLRIRQPDLPRPYRTTWYPLPPLVYLAITG
                     WTLGYIIAHRPKEGLWGLAIIAGGALLYLLIRRGRPTEEVSS"
     gene            complement(3674..4114)
                     /locus_tag="AC16UZ_05685"
     CDS             complement(3674..4114)
                     /locus_tag="AC16UZ_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246209.1"
                     /translation="MARLALTGAIIILLSGCAEPFIVFAGGALSGDEKVPPADWSFLE
                     AIDTVQIETDPNDPYSVNVWSVGIGADVYVATGEDGTNWTERIDGDPSVRLRTNGDIY
                     PLRATAVTEMAERRRVAQAYLNKYDVDDDEGWVDTGKIYRLDRR"
     gene            4244..5224
                     /locus_tag="AC16UZ_05690"
     CDS             4244..5224
                     /locus_tag="AC16UZ_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR6246210.1"
                     /translation="MRAMQLTEPGGDFRLVDLPKPRPAAGEVLLAVHACGVCRTDLHV
                     VDGDIVDGRYPVVPGHQIVGVVEQAPDGDLQPGQRVGVPWLGHTCGTCVFCLAGQENL
                     CDSAEFTGYHRQGGFAEYAVADARFCFPIPESYPDVQAAPLLCAGLIGYRALGMVGDA
                     RKLGFYGFGNAAHILTQIAVWEGREVSAFTRPGDAAGQRFARGLGAVWAGDASTPPPE
                     PLDAAIIFAPDGALVPAALKHVRKGGVVVCAGIHMSDIPSFPYADLWGERIIRSVANL
                     TREDGVRLLEVAPRVPVRTEVQVFPLERTADALDALREGRVEGGAVVQVR"
     gene            complement(5221..6267)
                     /locus_tag="AC16UZ_05695"
     CDS             complement(5221..6267)
                     /locus_tag="AC16UZ_05695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6246211.1"
                     /translation="MRISMLLSYAGGFKEAVKEVAELEKAGLDLVWVPEAYSFDAPSA
                     MGYLAANTERVTIASGILPIYSRTPSLLAMTAAGIDYLSDGRCMLGLGASGPQVIEGF
                     HGVPYVAPASRIREIIEICRKVWRRERLQHDGRYYQIPLPQNKGTGLGKPLKLINHPV
                     REEIPIAIAALGAKSVEMTAELADAWLPAFYTADAADAVWGDALRAGAAKRDPGRAPL
                     EVFAGGAVAIGEGLEDLRNLARPQAALYIGGMGARQKNFYNDIFSRSGYAQEAKLIQD
                     LYLSGDKKGAEAAIPESYLANNSLIGPEGFVRDRLQALSESGVTALNVGFMGRTSQER
                     IRSCEQLRKLVDTL"
     gene            6392..>6751
                     /locus_tag="AC16UZ_05700"
     CDS             6392..>6751
                     /locus_tag="AC16UZ_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HIR6246212.1"
                     /translation="MDREFDVIVWGASGFTGRLVAEYLNERYGLGGSVAWAIAGRNQG
                     KLDAVRQEIGAPGLSTILADGNDEASLAAMAERARVICTTVGPYALYGSRLVAACVSR
                     GTHYCDLTGEVHWMRRMI"
BASE COUNT         1099 a         2148 c         2355 g         1149 t
ORIGIN      
        1 ccaggacgcg accgcactgg cggcccgtga ggtcttcggt gatgtgctct ggcagggcta
       61 cggtcagacg gaggtggtgc cggtgtccat gatgggcccg cgccagtggt tcgccgaggt
      121 ggagggctcc agccctctgc gggcatgcgg cctgccgctg ccgtttgcag aggtgcagat
      181 ctgggacgag gacaaccagc cggtggctgc gggcgaggtg ggcgagatcg tggcccgcgc
      241 ggacgggcag atgacgggtt tctggaacaa tcccgaggcg agtgccgaac gtatcgtgga
      301 tggctgggtc aagacgggcg atctcggcag gctggatgcc aatggctatc tgtacgtggt
      361 ggaccgggcg gacgacatga tcatctcggg gggctacaac atctggccgg cggagctcga
      421 gaacgttctc gccagccatc cggccgtcat cgaggtggcg gcattcggcg tgcccagcca
      481 gaaatggggc gagtcgccgt atgccgtttg cgtggtcggg aatgccgagg cggtcggcga
      541 ggccgagctc atcgatctgg ttgccgagga gctaggcagc tacaagaaac ccgtcggcgt
      601 gaccatcacc acggatcccc tgccaaagag cccggtcggg aagatcaagc gcaaggatct
      661 tcgcgagccc ttctggaagg gacacgagcg gcgcgtcgcc ggcagctgac cggttcaggc
      721 ctggccctca gtcatccatg ggcggcctgg cccggaggcg cttggtggtc ccccgcttgc
      781 gcttggtgtc cttgcgtcgt acccgctccc cggctggcgt tctggtggca acccggcgtc
      841 ggggcacgcg ctgcgcctgt tccagcagct cgctcaaccg gctcatcgcg tcggcccggt
      901 tgcgcgcctg ggtccggaag cggctggccg tgatgacgag ctccccgcgc tgattcacct
      961 ggcgcccggc cagcttctcc agtcgttcgc ggacggccgg cgggaggttg gcgcgatcga
     1021 gatcgacacg gagctgcacc gtggtggcga ccttgttgac gttctgccca cccgcgcccg
     1081 ggctccggac gaaacgctcg gagcaagcct cgggcgggat ctgcctgata cgtgaaacgc
     1141 tcatcggcca atcatagcag cggcgccttc ttcccagacg cgggccggca cgctaggcga
     1201 ggatggtctt gctcgattcg ccgggcacct cgcgtgccag cctgggcacc agatagccgg
     1261 gcagcctgcg acgcagaccg gccaccaggg cacgcccgcg cgctggcgcc acgtcgaagt
     1321 gggcggcgcc ggccaccgga tccagaaggt gcaggtagta gggcagcacg ccggcatcga
     1381 acagccgttg actcagctcg gccaggtcat cctcgttgtc gttgactccc ttgaggagca
     1441 cggactggtt cagaagctgg gcgcccgcgg ccttgaggtc cgccaacgcc cgtcgcaccg
     1501 aggcgtcgat ctcgttggca tgattgcagt gcacgacgaa aaccgtccgc aagcgggttg
     1561 cggccagcgt atcgaccagg cccgcgtcga cccgttcggg aatgatgatg gggaagcggg
     1621 tgtggaaccg cagggtttgg acgcttgcga cgttctccat ggcgcgaatc aactcgccga
     1681 tcgcctcgtt ggtcagcgtg agtggatcgc cgcctgaaag tatgacttcc gtcacctgcg
     1741 tgtccgcgct cagcgcgtcg acggcgcccg tccagcgttg gcgtgacgca agctggtccg
     1801 cgtaggggaa attcctgcgg aagcagtagc ggcagtggac gggacacgcg ccggaggcga
     1861 tcagcagcgc gcggccggaa tactttcgca cgacgccggc tcgggcaaag cgctgctcct
     1921 gcagcgggtc ggcttcgaag cccggcgccg tgatgcgctc tgccccatcg gggagaacct
     1981 ggcgcagcag ggggtcgtgg ggatctccct tgcgcatgcg tgccgcaaag gatctcggaa
     2041 ccagcatggg aaagtcatcg tcgtgaccgt ccatcaccgg cgcccaggcc gggtcgagct
     2101 ccagggcctc gatcagctcc cggggcgttc tgatggcgtt gcgcaacgcc tcctgccagc
     2161 tgccggtcgc cggatcgtcg gcgaccgtgc gtgtccgcag acgggccgcg gattgctcgg
     2221 gtgcactttg agtcatgggg ttcacgtccg ggacgagtat cgcgcaatcg tctgcggtct
     2281 catagcgcag ccggtcccgg tcccggtccc gggtcggccg tcgactcaag aagagacctc
     2341 ctccgtcggc cgcccccgcc ggatcaggag gtagaggagc gcgcctcctg cgatgatggc
     2401 caggccccac aacccctctt tcggtcggtg ggcgatgatg tagccgaggg tccatcccgt
     2461 gatggccagg tagacgagcg gcggtagcgg gtaccacgtc gtgcgatagg ggcggggcag
     2521 gtcgggctgg cggatgcgca gcacgaacag gcccagcacc gtgaagaacg tgctgacccc
     2581 gagggtgaag ccggcaaaca cgagaatggc ttcgaaagac gctgtgagaa tgaaggccag
     2641 cgccagcacg gactgaacga cgatggccgt caccggtacc ccgtcgccgc tcacccgagc
     2701 cagaaaacga aacgcggggt agtcctggcc gatcacctgc aaaacccttg gacccgccat
     2761 gatcatggcg ctggcggtgg atatcagaag cagagccaga atgacggcca tgatggccgc
     2821 gccgttcgag ccgaaggcgt gctcggcggc gatgtagccg atctccagcc tgccctccat
     2881 ggcctccgcc ggcgcgacgt acagaaaggt tgcattcaaa agcacgtaca gcagcattac
     2941 gaccacggtt ccctggagca gcacccggga gagtgtctgc tcggggcggt ccagctcgcc
     3001 cgtgaggtag gtggccgcgt tccagcctgt gtaggcgtag ttgacgtaga tcaaagccac
     3061 ggcgaacgcg ccgctggtga gcagcgctcc gtcaccctcg tggggcagca gcgatatccc
     3121 ctgaggctcg tcgaccagca gggcggcgag caggcagaaa ccgagaatca ggatgatctt
     3181 cacggcagtg aacgcccgct ggacgccgcc gctcgagcgt cgcgaggtgg cgtgagcaac
     3241 ggaaagcacc acgaccagac cgcacgccag ccagaccggc gacagcgcgg gaaagaccga
     3301 ggccagatag atgccgaagg taatggcggc gagcgccgtg ggtgcggcaa acccgatggt
     3361 ggcggagatc cagccggaca cgaagcctac ggccggatga tagattcgtc ccaggaagtt
     3421 gtactcgcca cccgagcgcg gcagagccgc gccgagctcc gcgtaggaca gcgccccgca
     3481 gagcgccgtc aaccccccca ccgcccagag catcagcagg acgaactcgg atctgatgtc
     3541 ggccagctga aagcccaggc tcgtgaacac gccggtaccg atcatgttgg ccatgatcac
     3601 ggccgtggct gtgcgcgcgc tgaatttcat gagcccgggg cctcaggcac ccgtcacggt
     3661 gagccggtcg cgactagcgc cggtccagac gatagatctt gccggtatcg acccagcctt
     3721 cgtcatcgtc gacgtcgtac ttgttcaggt aggcctgagc gacgcgcctg cgctcggcca
     3781 tctcggtgac ggcagtcgcg cgcagcgggt agatgtcgcc gttggtcctg agccttacgc
     3841 tcgggtcacc gtcgattcgc tccgtccagt tggtgccgtc ctcgcccgtg gccacgtaca
     3901 cgtctgcgcc gatcccgacg gaccacacgt tcacggagta ggggtcgttc ggatccgtct
     3961 ctatctgcac ggtatcgatg gcttccagaa aggaccagtc ggccggcggc accttctcgt
     4021 cgccgctgag cgcccccccg gcgaagacga tgaacggctc tgcacagccg ctcaggagga
     4081 tgatgattgc gcctgtgagt gcgagtcgcg ccatggcctg accttcgaga agacggcaat
     4141 tagggcgaat tctttgccgc ctgtaaatag atttctcgcc gtgatcggcc cacggaccgt
     4201 atgatctgag ggtccggtcg cttgcgtatg ggagggcgtg gacgtgcgcg cgatgcagct
     4261 tacggagccc ggtggagatt tccgcctggt ggacttgccg aagcctcgac ccgcggcggg
     4321 cgaggttcta ctggccgtgc acgcctgcgg cgtctgccgc accgatctcc acgttgtgga
     4381 cggggacatc gtcgacggcc gctatcccgt cgtgcccggg caccagatcg tcggggtggt
     4441 agagcaggcg ccggacggcg atctgcaacc gggacagcgg gtgggcgtcc cgtggctcgg
     4501 ccatacctgc ggcacctgcg ttttctgcct tgcgggccag gagaacctgt gcgattcggc
     4561 cgaattcacc ggataccatc gccagggtgg attcgcggag tacgcggtcg ccgacgcccg
     4621 cttctgtttc cccatcccgg aaagctatcc ggacgttcag gcggcgccgc tgctgtgcgc
     4681 ggggctgatc ggctaccggg ccctgggaat ggttggggat gctcgaaagc tgggtttcta
     4741 cgggttcggg aatgccgccc acatcctcac ccagattgcg gtatgggagg ggcgcgaagt
     4801 gtccgcgttt acccgcccgg gggatgcggc cggccagcga ttcgcgaggg gtctgggtgc
     4861 tgtgtgggcg ggtgatgcgt cgacgccccc tccggagccc ctggacgccg ccatcatctt
     4921 cgcgccggac ggggcgctgg tgccggcggc cttgaagcat gtgcgcaagg gtggggtggt
     4981 ggtctgcgcg ggcattcaca tgtccgacat cccttcgttc ccctacgccg atctctgggg
     5041 cgagcgcatc atccgctcgg tggccaacct gacccgcgaa gacggcgtgc ggttgctgga
     5101 ggtggcgccc cgcgtcccgg tccgcaccga ggtgcaggtt ttcccgctcg agcggacggc
     5161 ggatgcgctc gatgctctgc gggaaggccg cgtcgaggga ggcgccgtcg tccaggtgcg
     5221 ctagagcgtg tccaccagct tgcgcagctg ctcgcagctg cgaatccgct cttgtgacgt
     5281 tctgcccatg aagcccacgt tcagggcggt cactcctgat tcgctcagcg cttgcaggcg
     5341 gtctcgaacg aaaccctcgg gcccgatgag cgagttgttg gccaggtagc tctcggggat
     5401 agcggcctcg gcccctttct tgtccccgga caggtacagg tcctggatca gcttggcctc
     5461 ttgtgcgtac ccggagcggc tgaagatgtc gttgtagaaa ttcttctgtc gcgcgcccat
     5521 gccgccgatg taaagcgctg cctgggggcg cgccagattc cgcaggtctt cgaggccctc
     5581 gccgatggcc accgcgccgc cggcgaacac ttccagcggc gccctgcccg gatcccgctt
     5641 cgcggctccg gcgcgaagcg catcgcccca caccgcgtcc gcggcatcgg cggtgtagaa
     5701 ggcgggaagc caggcatcgg cgagctccgc cgtcatctcc acgctcttgg cgcctagtgc
     5761 ggcgatggcg atgggaatct cttcgcggac cgggtggttg atgagcttca gcggcttgcc
     5821 caggccggtg cctttgttct gcggcagcgg aatctggtag tagcgcccgt cgtgctgcag
     5881 acgctcgcgg cgccaaacct tccggcagat ctcgatgatt tcccggattc gggaggcggg
     5941 tgccacgtag ggcacgccgt gaaagccttc gatgacctga ggcccagacg cgccgagccc
     6001 gagcatgcag cggccgtccg agaggtagtc tatgcctgct gccgtcatgg ccagcagaga
     6061 cggtgtccgg gagtagatgg gcaggatgcc ggaggcgatg gtgacgcgct cggtgtttgc
     6121 cgccaggtag cccatggcgc tgggcgcgtc gaaggagtac gcctcgggaa cccagaccag
     6181 atcgagaccg gctttctcca gctcggcgac ttctttgacc gcttccttga aaccgccggc
     6241 atagctcagc agcatggata tacgcatgtt caaccccttg agaaggtgat ggttcgttgg
     6301 tcgccggccc ggccgcggag tatgcttggc ggctgccgcc cctttgccac cggcgtggcg
     6361 gtcgatgtta acagaatgag gatcactgcc gttggatcgg gaatttgacg tcatcgtctg
     6421 gggggccagc ggtttcaccg ggcgcctcgt agccgagtac ctcaacgagc gctacggatt
     6481 gggcggaagc gtcgcctggg cgatcgccgg gcgaaatcag ggcaagctcg atgcggtgcg
     6541 tcaggagatc ggcgcgccgg gactgtccac gatcctggcc gacggcaacg acgaggcatc
     6601 gctcgccgcc atggccgagc gcgcccgcgt catctgcacg acggtggggc cctatgctct
     6661 gtatggcagc cggctcgttg ctgcgtgcgt cagccgggga acccactact gcgatctgac
     6721 cggcgaggtc cactggatgc gccgaatgat c
//
LOCUS       DBNHOX010000256         2357 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_663828, whole genome shotgun sequence.
ACCESSION   DBNHOX010000256 DBNHOX010000000
VERSION     DBNHOX010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_663828"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /gene="cofD"
                     /locus_tag="AC16UZ_05705"
     CDS             <1..983
                     /gene="cofD"
                     /locus_tag="AC16UZ_05705"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HIR6246213.1"
                     /translation="RRRPHGLPTSARGSVSIMSGYLAITGGVGGAKLGLGLAQILPPD
                     ELTFAVNTGDDFEHLGLHIAPDVDTLTYTLAGLSNTETGWGRADETWDFMDALDGLGG
                     ETWFRLGDRDLALHVMRTHLLSAGACLTEVTDHICRALGVAHRVLPMSDDPVRTTVHT
                     ERGPLPFQHYFVRERCEPAVTGFEFAGIGQARLNPAITSRLDGLRGIIICPSNPFVSI
                     DPMLNLRGARAALKESGVPIVAVSPIVGGAAIKGPTAKMMRELNVPATAAQVAEHYGD
                     LLCGLVLDEQDAHLEGTLSTPTIVAQTVMVTLQDRVDLGRTVMDFIEQLS"
     gene            1022..1693
                     /gene="cofC"
                     /locus_tag="AC16UZ_05710"
     CDS             1022..1693
                     /gene="cofC"
                     /locus_tag="AC16UZ_05710"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HIR6246214.1"
                     /translation="MWAVVPLKQLEQAKARLANVLTAEERRALMLAMARDVLTALGRS
                     KRLTGILIVSRTPEADALAQAFGTERFAEGPGANLSQALVQASEHLASRLEARGVMIV
                     PADVPLITPAEIDAILEQQDRVTVVPDDENVGTNCLICSPPNCIPYVFDGSSFKPHVD
                     AAFSLGITPTIMPSAGFALDIDTPDDLRTLLDRDPACQTGSYLAKSGIAERLSDADNG
                     SLTSR"
     gene            1697..>2357
                     /gene="cofG"
                     /locus_tag="AC16UZ_05715"
     CDS             1697..>2357
                     /gene="cofG"
                     /locus_tag="AC16UZ_05715"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:TIGR03550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HIR6246215.1"
                     /translation="MALKTLIDDIRALALAGGLPSPAQALALADEPDTAALAAIAAEV
                     RDRGFQNVVTYSRKLFIPLTHLCRDVCHYCTFAQVPRKLKAPYMSVDEVLEIARHGAA
                     MGCKEALFTLGEKPELRYRAAREALAELGFETTLDYLAHVAEIVYRETGLLPHLNPGT
                     MTPEELARLRPLSPSMGIMLESTAERLGEKGMPHHGSPDKVPAVRLETLDHAGAARVP
                     FT"
BASE COUNT          408 a          836 c          725 g          388 t
ORIGIN      
        1 accgacgacg cccgcatggt ctgccgacct ctgcacgagg atctgtttcg ataatgtccg
       61 ggtatctcgc catcacgggt ggcgtgggcg gggcgaaact ggggctcggt ctcgcgcaga
      121 tcctgccgcc ggacgagctc accttcgccg tgaatacggg cgatgacttc gagcacctgg
      181 gtctgcacat cgcgccggac gtcgacaccc tcacctatac cctggcgggg ctgagcaaca
      241 ccgagaccgg ctgggggcgc gccgacgaga cctgggattt catggatgcc ctggacggcc
      301 tcggcggtga gacctggttc cgtctcggcg atcgggacct ggcgctgcac gtgatgcgaa
      361 cccacctgct gtcggccggt gcttgcctca ccgaagtcac cgaccacatc tgccgagccc
      421 tgggcgtcgc ccaccgggtg ctgccgatgt ccgacgatcc tgtgcgcacg accgtgcaca
      481 ctgaacgggg gcccctgcct ttccagcatt acttcgtgcg ggaacggtgc gagccggcgg
      541 tgaccgggtt cgagttcgcc gggatcgggc aggcgcggct caacccggcg atcacgtcgc
      601 gcctggacgg ccttcgcggc atcatcatct gcccctccaa cccgttcgtc tccatcgacc
      661 ccatgctgaa cctgcgcggc gcccgcgccg ctctaaaaga aagcggcgtg cccatcgtgg
      721 cggtctctcc catcgttggc ggagccgcca tcaaggggcc caccgcgaag atgatgcgcg
      781 agctcaacgt cccggcgacc gcggctcagg tggccgagca ctacggcgat ctgctctgtg
      841 gactcgtgct cgacgagcag gatgcgcatc tggaaggcac gctgagtacc ccgactatcg
      901 tggcccagac cgtcatggta acgttgcagg accgtgtgga tctcggccgc acggtcatgg
      961 atttcataga acaactcagc tgattcaagc tgaaaccaag caagacggag agaacctccc
     1021 catgtgggcc gtggtaccgt tgaaacagct cgaacaggcg aaggcccggc tggccaacgt
     1081 tctgacggca gaggagcgcc gggcgctcat gctcgccatg gcgcgcgacg tgctcacggc
     1141 cctcggccgc tccaagcggc tgacgggcat cctcatcgta tcccgtacac cagaagccga
     1201 cgccctggcc caggccttcg gcacggagcg cttcgcggaa ggccccggcg cgaatctttc
     1261 ccaggccctc gtccaggcga gcgagcacct cgcctcgcgg ttggaagccc gcggcgtgat
     1321 gatcgtgccc gccgacgtgc cgctgatcac gccggcggag atcgacgcga tcctcgagca
     1381 gcaagatcgg gtcaccgtgg tcccggacga cgagaacgtc ggcacgaact gcctgatctg
     1441 ctcaccaccc aactgcatcc cctacgtctt cgacggttcc agtttcaagc cccatgtgga
     1501 cgctgcgttc tccctgggca tcacgcccac catcatgccc tccgccggct tcgccctgga
     1561 catcgatacc cccgacgacc tgcgcacttt gctggaccgc gatccggcct gccaaacggg
     1621 ctcgtatctt gccaaatcag gtatagccga gcggcttagc gatgccgata atggctcgct
     1681 gacctcgagg taggaaatgg ccctgaagac gctcatcgac gacattcgcg ccctggccct
     1741 ggccggcggg cttcccagcc cggcacaggc cctcgcgctg gctgacgagc cggacaccgc
     1801 cgccctggcg gcgatcgccg ctgaagtacg cgaccgcggc ttccagaacg tggtgaccta
     1861 ttccagaaag ctattcattc cgctcaccca tctgtgtcgg gacgtctgcc actactgcac
     1921 gttcgcccag gtcccgcgga agctcaaagc gccctacatg agcgtggacg aggtgctcga
     1981 aatcgcccgt cacggcgccg ccatgggttg caaggaagcc ctgttcaccc taggcgagaa
     2041 acccgagctg cgttaccgag cagcccggga agcgcttgcc gaactgggtt tcgagaccac
     2101 gctcgactat ctggcccacg tggcggagat cgtctatcgg gagaccggcc tgctgccgca
     2161 cctcaacccc ggcaccatga cccccgagga gctcgcccgg ctgcgccccc tctcgccatc
     2221 catgggcatc atgctggaat ccacagccga gcggctgggc gagaaaggga tgcctcacca
     2281 cggctcgccg gacaaggtgc ccgccgtgcg tctcgagacc ctcgaccacg ccggcgccgc
     2341 gcgggttccg ttcacca
//
LOCUS       DBNHOX010000257         1991 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_791960, whole genome shotgun sequence.
ACCESSION   DBNHOX010000257 DBNHOX010000000
VERSION     DBNHOX010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_791960"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1276)
                     /locus_tag="AC16UZ_05720"
     CDS             complement(236..1276)
                     /locus_tag="AC16UZ_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR6246216.1"
                     /translation="MFKNIRGLFSRDLSIDLGTANTLIYVRDQGIVLNEPSVVAIRVQ
                     GNQKSVAAVGLDAKRMLGRTPGNITAIRPLKDGVIADFLVTEKMLKYFIAKVHENSFI
                     RPSPRVLVCVPCKSTEVERRAIRESALSAGARDVRLLEEPMAAAIGAGLPVQEAVGTM
                     VVDIGGGTTEIAIISLNGVVYSDTVRVGGDRFDESIVAYVRRTQGSLIGEATAERIKQ
                     EIGAAYPQKEVREIDVRGRNLAEGVPRSFTLNSNEILEALQEPLSMIVQALKSALEQC
                     PPELASDIAETGLVLTGGGALLRDLDTLLAEETGLPVIVAEDPLTCVARGGGKALELM
                     DKAGNADLLSTE"
     gene            1446..1727
                     /gene="gatC"
                     /locus_tag="AC16UZ_05725"
     CDS             1446..1727
                     /gene="gatC"
                     /locus_tag="AC16UZ_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003120927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR6246217.1"
                     /translation="MASIEIAHLCRLAQLALTESEQAAALADLQRIIAMIDEMQAMDT
                     DGVEPLAHPIESDQRLRPDTVSETVDRDHYQRGAPATRDGLYLVPRVVE"
     gene            1729..>1991
                     /locus_tag="AC16UZ_05730"
     CDS             1729..>1991
                     /locus_tag="AC16UZ_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR6246218.1"
                     /translation="MDPFASIGQHAARLRDGSYSSVELTRFFVDRAREHADLNAFICV
                     DEEGALAAAEAADARLRAGTATALTGIPLAHKDIFCTRGTITS"
BASE COUNT          350 a          667 c          631 g          343 t
ORIGIN      
        1 gagcagcgcg gagcgggtcg cgaacacgtt gccgagctcc tctcccacca ggtacggcgt
       61 ttccgccacg tagtgaaggg gagcgagcat ggtgccgaac acctggcggg tgccgcgcat
      121 gaaagaggtg gtgaggtcca gggtgaccag tatcagggat acgaggacga gcccggtgag
      181 caggaagcca gcgccagatt ctcgaacaaa cagcgctttg atggcttgag tctccctatt
      241 cggtcgacag caggtccgcg ttgccagcct tgtccatgag ctcgagggcc ttgccgccgc
      301 cgcgggcgac gcaggtcagc ggatcctcgg cgacgatcac cggcaagccg gtctcctctg
      361 ccaggagggt gtcaaggtca cggagcagcg cgccgccgcc cgtcagcacc aggccggttt
      421 ccgcgatgtc cgaggcgagc tcgggcggac actgctccag ggcgctcttc agagcctgca
      481 cgatcatcga caggggttcc tgcagcgctt ccaagatctc gttgctgttc agcgtgaagc
      541 tgcgcggcac cccttccgct aagttgcggc cgcgaacgtc gatctcgcgc acctctttct
      601 gcgggtaggc ggccccgatc tcctgcttga tgcgctccgc cgtggcttcg ccgatgaggc
      661 tgccctgggt acggcgcacg taggccacga tggattcgtc aaagcggtct ccaccgaccc
      721 gcacggtatc cgagtacacc acgccgttca gcgagatgat ggcgatctcc gtcgtgccgc
      781 cgccgatgtc caccaccatg gtccccaccg cctcctgcac cggcaggcct gcgccgatgg
      841 cggccgccat gggctcctcg agaagacgca cgtcccgcgc gccggcggaa agcgcgcttt
      901 cgcggatggc gcgccgctcg acctcggtgg atttacaggg cacgcagacc agcacccggg
      961 gactgggacg gatgaacgag ttctcgtgga ccttggcaat gaagtacttg agcatcttct
     1021 cggtgacgag aaagtctgcg atgacgccgt ccttcagggg ccggatggcg gtgatgttgc
     1081 ccggtgtgcg tcccagcatg cgcttcgcat ccagccctac cgcggccacg ctcttctggt
     1141 tgccctgcac ccggatggcg acgacggaag gttcgttcag cacgatgccc tgatccctga
     1201 cgtagatgag cgtgttggcg gtgcccaggt cgatggacag gtcccgggag aacagcccgc
     1261 gaatattttt aaacatctgg aataaggact cgggttaatt gactgaacac ttgatcccct
     1321 gacaagaggg gggatctcgg cggacggtac tctatcaatt tgcaacggca acgggaactg
     1381 cccggcacac cctcccgggc accggaaaat ggcttcagtg accgctttcc attacgcttc
     1441 gcgccatggc cagcatcgaa atcgcccacc tctgccgcct tgcccagctg gcccttacgg
     1501 agtcggaaca ggccgccgcg ctcgccgatc tgcagcgcat catcgccatg atagacgaga
     1561 tgcaggccat ggacaccgac ggcgtcgagc cgttggccca tccgatcgag tcggatcagc
     1621 gtctgagacc cgacaccgtc tccgagaccg tcgaccgcga tcactaccag cgcggcgcgc
     1681 cggccacccg ggacggcctg tacctcgtac cccgagtcgt cgagtaacgt ggacccgttt
     1741 gcttctatcg ggcagcacgc ggcccggctg cgagacggca gctactcgag cgttgagctc
     1801 acccgtttct ttgtcgaccg cgcccgggaa catgccgacc tgaacgcctt catctgcgtc
     1861 gacgaggagg gcgcgctcgc cgcggccgaa gctgccgatg cgcggctgag agcgggcacg
     1921 gccacggccc tcaccggcat ccccctggcg cacaaggaca tcttctgcac ccggggcacg
     1981 atcacgtcct g
//
LOCUS       DBNHOX010000258         1757 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1306750, whole genome shotgun sequence.
ACCESSION   DBNHOX010000258 DBNHOX010000000
VERSION     DBNHOX010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1306750"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="AC16UZ_05735"
     CDS             <1..1468
                     /locus_tag="AC16UZ_05735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6246219.1"
                     /translation="AHGRDHITHAELGLDAEGNFTALKVQTSANLGAYLSTFGSSVPT
                     YLYGTLMAGQYRTPNIYVEVQGIYTNTAPVDAYRGAGRPEATYVVERIVETAARELGT
                     DPAELRRRNMIRPDQFPYETPVALVYDTGDYEASLDRALALVDYAGFPARRAASEAAG
                     KRRGIGLACYIEACGLAPSQLAIQLGAGVGLYESGEVRVNPTGSVTVFTGSHSHGQGH
                     ETTFAQVVSDRLGVPFESVEVVHGDTGRVEFGLGTYGSRSLAVGGSALIAATDKVIAK
                     GKRIAAHMLEAQESDVEFADGNFVVRDSNQSLSFGEVAFAAYVPANYPEDLEPGLSEK
                     AFYDPENFTYPAGTHIAEVEIEEETGVVRLVNFVAVDDFGHVINPMIVDGQVHGGLAQ
                     GAGQALLEHGVYDDYGQLQTGSYMDYCMPRADDVPDFVVDTTVTPCTHNPLGVKGCGE
                     AGAIGSPAAIMNAVTDALGVNDISMPATAEKVWRALRA"
     gene            1477..>1757
                     /locus_tag="AC16UZ_05740"
     CDS             1477..>1757
                     /locus_tag="AC16UZ_05740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6246220.1"
                     /translation="MYQFNYHRPASVEEAVGLFESMDDGIYMAGGQTLIPTMKQRLAA
                     PTDVIDLSGIEALSGIEVSADRVSIGAFTVHDAVASSSEIRATLPVLAFL"
BASE COUNT          305 a          565 c          591 g          296 t
ORIGIN      
        1 cgcccacggt cgggatcaca tcacccatgc ggagcttggc ctggacgcgg aaggtaactt
       61 cacggcgctg aaagtgcaga ccagcgcgaa tctcggcgcc tatctgtcca cgttcggctc
      121 ctccgtcccg acctatctct acgggacgct gatggccggc cagtaccgga ccccgaacat
      181 ctacgtcgag gtgcagggca tctacaccaa caccgcaccg gtagacgcct accgcggagc
      241 cgggcgtccg gaggccacct atgtggtcga gcggatcgtt gagactgccg ccagggagct
      301 cggcacggat ccggcggagc tgcggcggcg caacatgatc cggccggatc agttccccta
      361 cgagacgccg gtggcgctgg tctacgacac cggcgactac gaagccagtc tggacagagc
      421 gcttgcgctc gtcgactacg ccggcttccc ggctcgccgc gctgcttccg aagcggcagg
      481 caagcggcgt ggcattggcc ttgcgtgcta catcgaagcc tgcggcctcg cgccctcgca
      541 gctggccatc cagctcggcg caggcgtcgg gctctacgag agcggcgagg tgagggtgaa
      601 cccgacgggt tccgtcaccg tcttcaccgg atctcacagc cacggccagg gtcacgagac
      661 cacctttgcc caggtggtgt ctgaccggct cggcgtgccg tttgagagcg tcgaggtggt
      721 gcatggcgac accggccgcg tcgagttcgg cctcggtacc tacgggtcga ggtcgttggc
      781 ggttggtggt tcggcgttga tcgcggccac ggacaaggtc attgccaagg gtaaacgcat
      841 cgccgcgcac atgctcgaag cccaggagag cgacgtggag ttcgccgacg gcaatttcgt
      901 cgtcagggac agcaaccagt cgctgtcctt cggggaggtt gcctttgcgg cgtacgtgcc
      961 ggccaattac cccgaggatc tcgagccggg actttccgag aaggctttct acgatcccga
     1021 gaacttcact tacccggcgg gaacgcacat tgcggaggtg gagatcgaag aggagacggg
     1081 cgtcgtccgg ctcgtcaact tcgtcgccgt cgacgacttc gggcacgtca tcaatccgat
     1141 gatcgtcgac gggcaggttc acggagggct tgctcagggt gccggacagg cgctgctgga
     1201 gcacggcgtg tacgacgact acggccagct gcagacaggc agctacatgg actactgcat
     1261 gccgcgcgcg gacgacgtgc cggacttcgt ggtcgacacc acggtgacgc cgtgcaccca
     1321 caaccctctc ggcgtcaagg gctgcgggga agcgggcgcc atcggctccc cggcggcgat
     1381 catgaacgcc gtcaccgacg ccctcggcgt caacgacatt tccatgccag ccacggccga
     1441 gaaggtgtgg cgggcgctgc gggcctagga gaagccatgt atcagttcaa ctaccaccgc
     1501 ccggcgtcgg tagaggaagc cgtcgggctg ttcgaatcta tggacgacgg gatctacatg
     1561 gccggggggc agacgctcat cccgacgatg aagcagcggc tggccgcgcc taccgacgtc
     1621 atcgacctgt cgggtatcga agcgctcagc ggtatcgagg tgagcgccga ccgggtcagc
     1681 atcggcgcgt tcaccgttca cgatgcggtg gcgagctcgt cggagattcg cgcgaccctt
     1741 cctgtgctgg ctttcct
//
LOCUS       DBNHOX010000259         2108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1178146, whole genome shotgun sequence.
ACCESSION   DBNHOX010000259 DBNHOX010000000
VERSION     DBNHOX010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1178146"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="AC16UZ_05745"
     CDS             complement(<1..564)
                     /locus_tag="AC16UZ_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6246221.1"
                     /translation="MTGFAIVLAKECRDNIRDRRTIISSLSLAVLGPALFVGLMAFVL
                     DSAVGESSDPVEITVVGADHAPGLMAFLETQNTRIERVDLEDPRGAVAGGSRKLVLSI
                     PDDYSERFAAGQVTTVSLIYDSSDMGMSRRNFRMAQQFILAHARMIGLLRLQLRGVDP
                     AIASPVQVQAVDTASPAARALAILATLP"
     gene            complement(561..1286)
                     /locus_tag="AC16UZ_05750"
     CDS             complement(561..1286)
                     /locus_tag="AC16UZ_05750"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6246222.1"
                     /translation="MIRAEGLDKSFGEVTAIRHVSFDAADGKVTALLGPNGAGKSTSL
                     RILATVLQPDGGKAEVDGVDVVADPMAVRRQIGVLPHAAGLYARLTARENVAYFGSLH
                     GIAKSRLSERIDELIELLEMGEFAERRVAGFSQGQRIKVALARALVHDPRNVLLDEPT
                     NGLDVMATRALRDIIIRLRDEGRCVLFSSHIMQEVANVCDDVIVIGHGRVLFQGTLDG
                     LRDHAGEADLEEAFIRVIDEEDV"
     gene            complement(1283..>2108)
                     /locus_tag="AC16UZ_05755"
     CDS             complement(1283..>2108)
                     /locus_tag="AC16UZ_05755"
                     /inference="COORDINATES: protein motif:HMM:NF019982.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6246223.1"
                     /translation="GVVPPGLALGVNVAENAQRRLDELFAQCDRAPECSEAFPDLQAS
                     FTQLQSNLNAEPVSLRFPNPITGLEESLAFGYDHLALVVRLLSYAPETASLLPLIIDQ
                     AAGGNYLPAASQALRITDALSGTMSFGMHNSVTCTEDLPFIDFNQVNWDALEATYIGA
                     DQVRALRAICEIWPRGAMDPDLKDPVQADTPTLILSGELDPITPPAYGDQIKPGFGNS
                     MHIIGAGQGHGVVSRGCLPGLVTQMVEAGHLGDLDAGCVERLSAAPFFIDLMGPAP"
BASE COUNT          371 a          677 c          719 g          341 t
ORIGIN      
        1 gggcaaggtg gctaggatgg ccagcgcgcg ggcggccggc gaggccgtgt cgactgcctg
       61 aacctgaacg ggggacgcga tggccgggtc gaccccgcga agctggagcc ggagaagccc
      121 gatcatcctg gcgtgcgcca ggatgaactg ctgcgccatc cgaaagttgc ggcgggacat
      181 gcccatgtcc gagctgtcgt agatgaggga cacggtggtc acctggcccg ccgcgaagcg
      241 ctcggaataa tcatcgggaa tcgacagaac cagctttcgg gatccgccgg ccaccgcacc
      301 ccgggggtct tcgagatcca cccgctcgat gcgcgtgttc tgggtttcca ggaaggccat
      361 cagcccgggc gcgtggtcgg cgccgacgac ggtgatctcc accggatcgc tggactctcc
      421 caccgcggag tccagaacga aggccatcag cccgacgaac agcgcgggtc ccaggacggc
      481 gagagacagc gacgagatga tcgttcgacg gtcgcgaatg ttgtcgcggc attccttggc
      541 aaggacgatg gcgaaaccgg tcacacgtcc tcctcgtcga tcacgcggat gaaggcctcc
      601 tccagatcgg cttcgccggc gtggtcgcgg agcccgtcca gggtcccctg gaaaagcacg
      661 cgtccatggc cgatgacgat gacgtcgtca cagacgttgg cgacctcctg catgatgtgg
      721 ctcgagaaca ggacgcagcg cccctcgtcg cgaagccgga tgatgatgtc tcggagcgcc
      781 cgcgttgcca tgacgtcgag cccgttggtg ggctcgtcca gcaggacgtt gcgcgggtcg
      841 tggacgagcg cgcgggcaag agcgaccttg atccgctgac cctgggaaaa accggcgacc
      901 cggcgttcgg cgaactcgcc catttctaaa agctcgatca gctcatcgat gcgttcgctc
      961 agccgtgact tcgcgatgcc gtgcagagac ccgaagtacg cgacgttctc ccgggccgtg
     1021 aggcgggcgt acagccctgc ggcgtgcggc agcacgccga tctgacgccg caccgccatg
     1081 gggtcggcga cgacgtcgac gccgtcgacc tcggctttcc ccccatcggg ttgcagcacg
     1141 gtggccagaa tcctgagcga ggtggacttc ccggcgccgt tggggccgag cagggcagtg
     1201 actttgccgt cggccgcatc gaacgagacg tgacgaatgg cggtgacctc gccaaacgat
     1261 ttgtcgagcc cctcggcgcg gatcatgggg ctggacccat caggtcgatg aagaatggtg
     1321 ccgcggacag gcgctcgacg cagccggcgt ccaggtcccc caggtgccct gcctccacca
     1381 tctgcgtgac gagacccggc agacatcccc tggatacgac gccgtgaccc tgacccgcac
     1441 caatgatgtg catcgagttg ccgaagcccg gctttatctg gtccccgtat gcgggcggtg
     1501 tgatggggtc cagctcgccg gacaggatga gcgtgggcgt gtcggcctga acgggatcct
     1561 tcaggtccgg atccattgcc ccgcgcggcc agatctcgca gatcgcgcga agggcccgga
     1621 cctgatcggc gccgatatag gtcgcctcca gagcatccca gttcacctgg ttgaaatcga
     1681 tgaagggcag atcctcggtg caggtgaccg agttgtgcat gccgaagctc atggtgccgc
     1741 tcagcgcgtc cgtgattcgc agtgcctggg acgccgccgg cagatagttg ccgccagccg
     1801 cctggtcgat gatcaggggc aaaagcgatg cggtttccgg ggcgtagctc aacaggcgca
     1861 ccaccagggc gagatggtcg tagccgaacg ccaggctttc ttccaggccg gttatcggat
     1921 tggggaaccg caagctgacc ggctcggcgt tcagattgct ctgcagctgg gtgaaactgg
     1981 cctgaaggtc aggaaacgcc tcgctgcact ccggtgcccg gtcgcactgg gcgaacagct
     2041 cgtccaggcg ccgctgcgcg ttctcggcca cgttgacgcc gagagccagc ccgggaggca
     2101 ccacgccg
//
LOCUS       DBNHOX010000260         3128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1371044, whole genome shotgun sequence.
ACCESSION   DBNHOX010000260 DBNHOX010000000
VERSION     DBNHOX010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1371044"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="AC16UZ_05760"
     CDS             complement(<1..245)
                     /locus_tag="AC16UZ_05760"
                     /inference="COORDINATES: protein motif:HMM:NF022853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HIR6246224.1"
                     /translation="MRRFWPGLGAAAIGFTLLLAAPGAAASALDDISTGAAGWLSEQP
                     TFLPVDEAFAMSAEVGPDGAILVRWDIADGYYLYRHR"
     gene            459..1079
                     /locus_tag="AC16UZ_05765"
     CDS             459..1079
                     /locus_tag="AC16UZ_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246225.1"
                     /translation="MPEGARADPLTEPAPAPAPHAAEIDALLLQANDAMERGQYINPA
                     RGSALGLYDRVLALNPGNAEARRGLELIAQHYLDLASAAVRRRQLTRARSMVDWARIV
                     DAEHPGIAAAEAQIRLLAEARRVRLPVAGSQLRDRSDALARSLAELGSRARNGNCLAI
                     IRSPSDASGRWIYQQMSRAPGTARIRADIEIGSPPMVELLCFENDA"
     gene            1097..1537
                     /locus_tag="AC16UZ_05770"
     CDS             1097..1537
                     /locus_tag="AC16UZ_05770"
                     /inference="COORDINATES: protein motif:HMM:NF016221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HIR6246226.1"
                     /translation="MAAALAAALAAAPVAIPASSAPALAGSELVVTDARVRALLPGQR
                     HTVAYFAIENRGPASVTLVSAESAAAGAMEIHESRRDGDMYRMRRRAEVEIPARTTVR
                     FDPGGLHLMLFDVQALGEQMEIELRTSDGRRLKAIFNRIAAGAE"
     gene            1534..2580
                     /locus_tag="AC16UZ_05775"
     CDS             1534..2580
                     /locus_tag="AC16UZ_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009041985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HIR6246227.1"
                     /translation="MKINWRFWASSNDADVTDGRLATAGRIGLGAVAAYLLVCVLLGW
                     WWDKEPDVFAVRENASSRAELGNEPLVTGYITTATLIRLGETLLDKRGGYLSNDVFPP
                     GVWMDNIPNWEFGVLTHIRDATRTYRNELSRSQSQSAEDPDIVRAEGKFFIDNASWFI
                     PQAESEYRDGIKALSSYLQRLADPTQPDAQFYARADNLQQWLQIVEARLGSLSQRLSA
                     SVGKRQLDVSLAGDAAASQATESPREQEVKTPWLEIDDVFYEARGQTWALLHLLRAME
                     QDFGDVLDKKNARVSLQQIIRELEPTQDTVWSPIILNGDGLGLLANHSLVMASYVSRA
                     NAAIIDLRALLSQG"
     gene            2763..>3128
                     /locus_tag="AC16UZ_05780"
     CDS             2763..>3128
                     /locus_tag="AC16UZ_05780"
                     /inference="COORDINATES: protein motif:HMM:NF015019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HIR6246228.1"
                     /translation="MNNIALIPPIFGIVGLIVAFFIFSLVKRYDEGTEEVKRIGDAIH
                     EGAMVFMRREYTMLAGFAAALIVILYIALGTETTIAFIVGALASATAGWIGMYTATKA
                     NVRTTTAAHTAGAAQALSVA"
BASE COUNT          563 a         1086 c          991 g          488 t
ORIGIN      
        1 aatcggtgcc ggtagaggta gtagccgtcc gcgatgtccc agcgaacgag tatcgcgccg
       61 tcgggcccga cctcggcgct catggcaaac gcctcgtcca ccgggagaaa cgtcggctgc
      121 tcggatagcc acccggcggc ccccgtggag atgtcgtcca gggccgacgc ggccgcaccg
      181 ggcgcggcca gcagcaaagt aaaaccaatc gccgcggcgc caagccccgg ccagaatcgc
      241 ctcattgttc gacggactct catcacctca attctatccg acacctcgac cgcattggag
      301 ttcgcccgca gacccggcaa tatagccgtt cccttggccc gcagaagtga actggtcggt
      361 tgcttcccag tgaacgaaca ctcccagcgc tctggctcgc cctgatgctc tgggtggtgg
      421 acgccgctgg actctcggcg caggccggag gtgattcttt gcccgaagga gcgcgggccg
      481 accctctcac cgagcccgcg cccgccccgg cccctcacgc ggccgagata gacgcgctgc
      541 tgctgcaggc caacgacgca atggaacggg gacagtacat caaccccgcc cgcggaagcg
      601 ccctgggcct ctacgaccgg gtactggcgc tcaatccggg caatgccgag gcgcgcaggg
      661 gcctggagct catcgctcag cattatctcg acctcgccag cgccgcagta aggcggcgtc
      721 agctgacccg cgcccggtcc atggtcgact gggccagaat cgtggacgcc gaacatcccg
      781 gcatcgccgc ggccgaggcg cagatccgcc tgctcgcaga ggcgaggcgc gtgcgcctgc
      841 cggtggcagg cagccagctg cgcgatcgca gcgacgcgct ggctcggtcg ttggcggagc
      901 tcggatcacg cgcgcgcaac ggaaactgcc tggcgatcat ccggtcgccc agcgacgcct
      961 cgggacgctg gatataccag cagatgagcc gcgctccggg aactgcgcgc atccgggccg
     1021 acatcgagat cggctcaccg cccatggtgg aactgctttg cttcgaaaac gacgcctgat
     1081 gcccgatgcc gccatattgg ccgccgcgct tgccgccgcg ctggccgccg cgccggttgc
     1141 catcccggcc agcagcgcgc ccgccctggc gggttccgag ctggtcgtga ccgatgcgcg
     1201 ggtccgggcg ctgctgcccg gccagcgcca tacggttgcc tacttcgcga tcgaaaaccg
     1261 cggccccgca agcgttaccc tggtgagcgc cgagagcgcg gccgccgggg ccatggagat
     1321 tcacgagtct cgccgcgacg gcgacatgta ccggatgcgg cgccgagcag aggttgagat
     1381 tccagcccgg accaccgtac gcttcgaccc gggcgggctg catctgatgc tgttcgacgt
     1441 ccaagcgctc ggcgagcaga tggaaatcga gctgcgcacg agcgacgggc gccgcctgaa
     1501 ggcaatcttt aatcgcatag cagcgggagc ggaatgaaga tcaactggcg attctgggct
     1561 tcttcgaacg atgcggacgt caccgacggg aggctcgcaa ctgccggtcg catcgggctg
     1621 ggcgccgttg ccgcctacct gctcgtctgc gtgctgctgg gctggtggtg ggacaaggaa
     1681 cccgacgtgt ttgccgttcg cgagaacgca tcgagccgcg cggagctcgg caacgagccg
     1741 ctggtcaccg gttacatcac caccgccacc ctgatccgcc tgggcgaaac cctgctcgac
     1801 aaacgcggtg gctatctttc caacgacgtc tttccgccgg gcgtgtggat ggacaacatt
     1861 ccgaactggg aattcggggt gctcacccac atacgggacg ccacccgcac ctaccgcaac
     1921 gagctttcgc gctcccagag ccagtcggca gaagaccccg acatcgtcag agcggagggc
     1981 aagttcttca tcgacaacgc gagctggttc atccctcaag cggagtcgga atatcgcgac
     2041 ggcatcaaag cgctctcctc ttacctgcag cgcctggccg accccaccca gcccgatgcc
     2101 cagttctacg cgcgggcaga caacctccag cagtggctgc agatcgtcga agcccggctc
     2161 ggcagcctgt cccagcgctt gtccgccagc gtgggcaaac ggcagctcga cgtctccctg
     2221 gccggagatg ctgcggccag ccaggccact gagtccccgc gggagcagga ggtcaagacg
     2281 ccgtggctgg aaatcgacga cgtcttctac gaggcccgag gccagacctg ggcgctgctc
     2341 cacctgctcc gggccatgga gcaggacttt ggcgacgtgc tggacaagaa gaacgcccga
     2401 gtcagcctgc agcagatcat ccgggagctc gagcccaccc aggacacggt ctggagcccc
     2461 atcatcctca acggcgacgg gctcgggttg ctggccaacc actcgctggt catggcgagc
     2521 tacgtctccc gggcgaatgc cgccatcatc gacctgcggg cgcttctctc ccagggctga
     2581 ccggcggcgg gtcccataca aattcagaca aatcccgccg cgcgctaggt tcttattcgc
     2641 agaccgctcc gttagaattc gcgccgaacc gagcgctgcc tccgcgcgta tcgtgcagca
     2701 acaagcacgg ggtggagcag atcgcccgcg ttccacacat ggtcatcgag ttggggaatc
     2761 caatgaacaa tattgcgctg ataccgccaa tattcggtat cgtcggcctg atcgtagcct
     2821 tcttcatctt cagcctcgtg aagcggtacg acgagggcac cgaggaagtc aagcggatcg
     2881 gggacgccat tcacgagggg gccatggtct tcatgcgccg ggagtacacg atgctggcgg
     2941 ggttcgcggc cgcgctgatc gtcatcctct acatcgccct cggaacagaa accaccatcg
     3001 ccttcatcgt cggcgcgctg gcctccgcga cggcaggctg gatcggcatg tacacggcga
     3061 ccaaagccaa cgtcagaacg actacggccg cccacaccgc cggcgcggcc caggcgctgt
     3121 ccgtggcc
//
LOCUS       DBNHOX010000261         2081 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_150301, whole genome shotgun sequence.
ACCESSION   DBNHOX010000261 DBNHOX010000000
VERSION     DBNHOX010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_150301"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..582)
                     /locus_tag="AC16UZ_05785"
     CDS             complement(262..582)
                     /locus_tag="AC16UZ_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6246229.1"
                     /translation="MIEQMTPAEFVRRRDAGEHLVLLDVREPHELARASVKGALHIPM
                     AQIPERLADLDRDRPIVVMCHSGGRSQAVVGFLLHRGFERVANLSGGISRWSREVDPS
                     VPLY"
     gene            complement(589..1245)
                     /locus_tag="AC16UZ_05790"
     CDS             complement(589..1245)
                     /locus_tag="AC16UZ_05790"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF013315.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase
                     family protein"
                     /protein_id="HIR6246230.1"
                     /translation="MNFDVESARRQMIDQQVRAWDVLDDRVLETLAAVPREHFVPEAY
                     REVAFADTAIPIGHGQTMFSPQVEGRCLQTLAIRREDDILEIGTGSGYFAACLARMGR
                     QVRTLEIFADLAQAADRVLTAHSVTNVTVESADASRLPVEPRYDVIALTASLPVYDAR
                     FERALKPGGRLFVVVGPGPVMEARLVTRVAEEQWARESLFETAIPPMINAVRPDAFNF
                     "
     gene            1463..>2081
                     /locus_tag="AC16UZ_05795"
     CDS             1463..>2081
                     /locus_tag="AC16UZ_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR6246231.1"
                     /translation="MTGLEFATGGLVLLVAILLAMVARQRRELARAAEVAEAVSAAAL
                     ARNGELPADEAIDDTLPSRFARAARQLAERGEADSGRSMLRALVASVHEPVLVHRERI
                     DAANPALCTLLGLEPGEVIGRTLAELVSPEYAGLVGSSVARRLAGEEAPEVTEIEVAD
                     AHGQVTRLELKGTTLVIGQDRVIVFSAIEMLPVAAGNGTPRTDGRA"
BASE COUNT          359 a          665 c          718 g          339 t
ORIGIN      
        1 ggttggcctc ggcctcgcgg atctggggat cgttctgcag ggcgcgggcg taaacctccg
       61 cgaggtcgct ggcctgcacc gatccggccg agaatagcgc agcgatgacg gcaatgtgga
      121 gggcaaaagt tagatttttc atgcacttac tctactgggt agtcgcccga ggaatactac
      181 tgttgtagta tggtatatca ctaactaacc cgatttcaac tgcttgtttt caacccggcc
      241 agaccccctc ccggggaagc gtcagtatag gggcacagag gggtccacct cccggctcca
      301 gcgggaaatc ccgccggaca ggttggcaac ccgctcgaag ccccggtgca gcaggaagcc
      361 gaccaccgcc tggctgcgcc cgcccgaatg gcacatgacg acgatcgggc gatcacgatc
      421 gagatccgcg agccgctcgg ggatctgcgc catcgggata tgcagggctc ccttcacgga
      481 ggccctggcc agttcatgcg gctcgcgaac atccagcagg acgagatgct cgcccgcgtc
      541 ccgtcgccgt acgaattctg cgggggtcat ctgctcgatc atgtggcctc agaagttgaa
      601 agcatcgggt cgaacggcgt tgatcatcgg cgggatggcc gtttcgaaca ggctctcccg
      661 cgcccactgt tcctccgcca cgcgggttac gaggcgcgcc tccatgaccg gcccggggcc
      721 caccacgacg aacaagcggc cgcccggttt cagcgcgcgt tcgaagcggg catcgtagac
      781 cggcaatgac gccgtcagcg cgatcacgtc atagcgcggc tcgacgggaa gcctggacgc
      841 atcggcagat tccacggtga cgttggtcac cgagtgggct gtcaggacgc gatcggcggc
      901 ctgggccagg tccgcaaaga tctcgagcgt ccgtacctgg cgacccatgc gggccaggca
      961 ggcggcgaag tagccgctgc cagtgccgat ctccaggatg tcatcttcgc gccggatggc
     1021 cagcgtctgc aggcatcgtc cctcgacctg tggggagaac atggtctgtc catgcccgat
     1081 ggggatcgcg gtgtccgcaa aggcgacctc gcggtacgct tcgggcacga aatgctcgcg
     1141 cggcacggcg gccagggttt cgaggacccg gtcgtccagt acgtcccagg cgcggacctg
     1201 ctggtcgatc atctggcgac gcgcggattc gacatcgaaa ttcatgggcg aactcgtggc
     1261 agggcggcat ccgggaggca gggaaggtta aggccaacgt ccgggaggaa caagccggtc
     1321 ttgaacacca ttatggtgaa tcgccacggg tctctccacc accttacgat atgctccgaa
     1381 atgtcgtgta ctgacaacat acaagggcca ggtgagctgc gggtcttccg catgacctgc
     1441 gccccagggg aaacgcgacg gcatgaccgg gcttgaattc gccaccgggg ggctggtgtt
     1501 gcttgtggcc atcctgctgg cgatggtggc caggcagcgg cgcgagcttg cccgcgccgc
     1561 cgaggtggcc gaggccgtct ccgcggccgc gctcgcccgg aatggagagc tgcccgccga
     1621 cgaagccatt gacgacacgc tgccctcgcg ctttgcacgg gcggcccggc agcttgcgga
     1681 gcgtggcgag gcggattccg ggcgctcgat gctccgcgcc ctggtggcgt cggtgcacga
     1741 accggtcctc gtgcaccgcg agcgaatcga tgccgcaaac ccggccctgt gcaccctgct
     1801 cgggctcgaa cccggggagg tgatcggcag gacgctcgcg gagctggtct ccccggagta
     1861 cgcgggactg gtgggctcca gcgtggcgcg ccgcctggcc ggtgaggaag cgcccgaggt
     1921 cacggagatc gaggttgccg atgcccacgg gcaggtcacc aggctggagc tgaaggggac
     1981 gaccctggtc atcggccagg accgcgtcat cgtcttctcg gcgatcgaga tgctgccggt
     2041 ggcagcgggg aacgggacgc cgcgcacgga cgggcgcgcc c
//
LOCUS       DBNHOX010000262         2162 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1114748, whole genome shotgun sequence.
ACCESSION   DBNHOX010000262 DBNHOX010000000
VERSION     DBNHOX010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1114748"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="AC16UZ_05800"
     CDS             <1..421
                     /locus_tag="AC16UZ_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6246232.1"
                     /translation="AIDYKNEDVNARLRELAPDGVNVFFDNVGGPILDAVLDNIAMRA
                     RVVICGAISQYDDLDNVTGPSLYLRLAERQSTMEGFAFFHFPESIGPATEELARWLAD
                     GALVFDEEILEGIERYPEALGFMFEGGNIGKLLVKAR"
     gene            481..1098
                     /locus_tag="AC16UZ_05805"
     CDS             481..1098
                     /locus_tag="AC16UZ_05805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6246233.1"
                     /translation="MSEDFSLREAIYNCRAMRRIRPDPVPEELLVELIDAANQAPSGS
                     NSQRARWIVVRDPAVKRQLADQNRRHAEPYIAADVSNPATPKLKRMLDAVVWQMEHMH
                     EIPALIVACFDYPEKVEGMGVYRSAGSVWPGIQNLLLTARALGLGAAPTTLALRDQDA
                     VRKILKLPETFAALCLIPVGYPMGKFGPVTRKPVSEVMRFDAWND"
     gene            1140..1517
                     /locus_tag="AC16UZ_05810"
     CDS             1140..1517
                     /locus_tag="AC16UZ_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HIR6246234.1"
                     /translation="MTRWIAVLLMLLWCRPALGGDIGGTWKNDADPVWIEVVHDDGVA
                     TGTVVRNDRNPAAAGRLLLKDLKPGDEANVWRGQVFAVRLGEFRDAQVRLTGDDRMEI
                     LVKVGFMSRTVTWTRADQAVGAE"
     gene            complement(1548..>2162)
                     /locus_tag="AC16UZ_05815"
     CDS             complement(1548..>2162)
                     /locus_tag="AC16UZ_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246235.1"
                     /translation="PALTIDPATLAGDGSAIDYCDLPLLDGSGKAAADIPKGNTPGCG
                     YDHFPLPILASCTEQLTEGAADIRGLWVGVEGGFVGHVERVEQCGSRTVVTSSGIIHD
                     SGPNSTAGLNTNDTEGAVLFIIGEKEYCPRTSASMNWNEGVLEFHAFGWGPVVVRRYL
                     EGERLVWEYLDGSVTRMERICRLPEGQKVPNPRGPRFSIFASKE"
BASE COUNT          370 a          674 c          714 g          404 t
ORIGIN      
        1 tgcgatcgac tacaagaacg aggacgtcaa tgcccgcctg cgcgagctgg cgcccgacgg
       61 ggtgaacgtc ttcttcgaca acgtgggcgg ccccatcctg gatgccgtgc tggacaacat
      121 cgcgatgcgg gcgagggtcg tcatctgtgg agccatctcg caatacgacg atctggacaa
      181 cgtcaccggc ccctcgctct accttcggct ggccgagcgc cagtccacca tggaagggtt
      241 tgcgttcttt cacttccctg agagcattgg accggcgacc gaggagcttg cgcggtggct
      301 ggccgacggc gcgctcgttt tcgacgagga gatcctggaa ggtatagagc gctaccccga
      361 ggcgcttggg ttcatgttcg aaggcggcaa cataggcaag ctgctcgtga aggcgcgttg
      421 accaacgggg tcgccgcact ttgcaccctc ttttgattcg taaactcaca ggaagccaag
      481 gtgtctgaag acttctcact tcgcgaggcg atctacaact gccgggccat gcgccggatc
      541 aggcccgacc ccgttccgga ggaactcctc gtcgagctga tcgatgcggc gaaccaggcg
      601 ccgtcgggat cgaactccca gagggcgcga tggatcgtcg tcagagaccc cgcggtcaag
      661 cgtcagctgg cggaccagaa ccgcaggcac gcagagcctt acattgctgc cgacgtctct
      721 aacccggcca cgccgaagct gaagaggatg ctcgatgccg tcgtatggca gatggagcac
      781 atgcacgaga taccggcgct gatcgtcgcc tgtttcgatt accccgagaa ggtggagggc
      841 atgggcgtgt accgcagcgc cgggtcggta tggcctggca tccagaacct gctgctgaca
      901 gctcgcgctc tgggccttgg cgcggcgccc accaccctcg cgctccgcga tcaggatgcc
      961 gttcggaaaa ttctgaagct gcctgagacc tttgccgcgc tctgcctgat cccggtcggc
     1021 tatccgatgg ggaagttcgg gcccgtcacc cgaaagcccg tttctgaggt catgcggttc
     1081 gacgcctgga acgactagcg gccggtattt tgcccgccgg aaggaggaga aaccgacccg
     1141 tgacgagatg gattgctgtt ctgctgatgc tgctgtggtg ccggccggca ctcggtggtg
     1201 acatcggcgg aacctggaaa aacgacgccg atcccgtgtg gatagaggtc gttcatgacg
     1261 acggcgtggc caccggcacc gtggtccgca acgaccgcaa tcccgcggcg gccggccggc
     1321 tgctgctcaa agatctgaag ccgggcgacg aagcgaacgt ctggcggggt caggtcttcg
     1381 cggtgcggct gggcgagttc agggacgccc aggtgcgctt gaccggcgat gaccgcatgg
     1441 aaatcctggt caaggtcggt ttcatgagcc gtacagtgac ctggacgcgg gccgaccagg
     1501 ccgtgggcgc ggagtaagta gcctttgggg gcgggtcgcc gggcccatta ctccttgctg
     1561 gcgaagatag agaaccgcgg gcctcgcggg ttcggcactt tctggccttc cggtaatcgg
     1621 cagattcttt ccattctggt cacgctgccg tccaggtatt cccagaccag ccgctcgccc
     1681 tccaggtagc gacgcaccac gacgggtccc cagccgaagg cgtggaactc gagtaccccc
     1741 tcgttccagt tcatgctggc cgacgtgcgc gggcagtatt ccttttcacc gatgatgaag
     1801 agcacggcgc cctcggtgtc gttggtgttc aggcctgccg tgctgttcgg tccggagtcg
     1861 tgaatgatgc cgctgctggt cacgaccgtg cgggagccgc actgctccac gcgttccacg
     1921 tgcccgacga acccgccttc caccccgacc cacaggcctc gaatgtcggc tgcaccctcc
     1981 gtcagttgct cggtacagct tgccagtatc ggcaggggaa agtgatcgta gccgcagccg
     2041 ggcgtgttgc ctttgggaat gtcggcggct gccttcccgc tgccgtcgag gagcggcaga
     2101 tcgcagtagt cgatggcgct gccgtccccg gccagggttg ccgggtcgat ggtcagggct
     2161 gg
//
LOCUS       DBNHOX010000263         3924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1307491, whole genome shotgun sequence.
ACCESSION   DBNHOX010000263 DBNHOX010000000
VERSION     DBNHOX010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1307491"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="AC16UZ_05820"
     CDS             <1..777
                     /locus_tag="AC16UZ_05820"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR6246236.1"
                     /translation="SADLIATIEGFSREDVDGYAVESQTRAARSIAEGRFEKALIPIF
                     NDDGSPALDRDEHPRPGTTLESLADLKPSFAAMGSQQVEGFPKPFDEMARDRYPQVEE
                     INHVHHAGNSSGVVDGASAVLVVSPDFAKAHGLRPRARIRMTATAGTEPLIMLTAPAP
                     AAQRCLQKAGMTRDDIDLWEINEAFASVVLKFMKEMGVDHDVVNIDGGAIALGHPIGA
                     TGGMLIGTAVDQLEREDKETALVAMCTGGGMGTATILERI"
     gene            complement(811..3375)
                     /locus_tag="AC16UZ_05825"
     CDS             complement(811..3375)
                     /locus_tag="AC16UZ_05825"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6246237.1"
                     /translation="MRAFSSFTAPLLMLLALSAFAQPTTDYLTPDLRARVERLKADVQ
                     QVPTNTTNVAERSRVLWNWANALSLDGGVLPVNLTQAVSFVFAYPDLMADRHDVIDGF
                     IQELTLLDEEPGALGTLRADSGPFQAASFVTLRQTFTVGSKAIQAGGGILVARHFQPN
                     YGAWQTGDPTADNYLSITTSAADASFTTETAPMIGMHGGFRRPADALVFRVASGTLRP
                     GDTVTLTYGDRSQGSRGFLMADFSSDMMPLPLYVAFTGSGRFFSLPIQPVAVIGGPAA
                     GVHAFAPSIVKAGEPFTLAVRAEDRFYNRASDGHPDWLVSLNGEPFREVPASEEPITL
                     LRNIEIAEPGVYRFTIASRDGQISGRGNPILVKQTAEERIFWGDTHGHSGFAEGIGTP
                     DFFMRWARDDARLDFVTHSEHDIWLDDGEWEQLRNNARSYTREGEFVAFLGYEWTVRN
                     THGGHHNVLFRTPAGRNRLAAPFYPSLSKLYAGLRGDAQPEDVLVIPHAHQAGDYRTS
                     DPELEHLVEVNSQHGNFEWFGQAYLRHGHQVGFTAASDNHLSQPGYGAPKNGTLAQRG
                     GLGAVIARARTTDALFDAMKQARAYATSGERIILEFSVNGTPMGQRGPFSETRTIEGR
                     VIGTAPIDDISLLRNGEEIWRRELLTDAADNTSSPGAFLLTFASDSDPRQPGDNPRGW
                     RHWRGTIEVRDATLIDASGMDFNNQLLQQIEADAGDPDLLRFSTITRGDTSSIRLDLE
                     DVSRATQVRVTLEPTQETGGAPATYGRHEQLPAAEVKLEIRDFENGETRAQLPAGPRE
                     DTITLRRIITQGSMDEAFTIDDVSGRQGDYYYVRVRQADDSLAWSSPVWIGGHAPR"
     gene            complement(3458..>3924)
                     /locus_tag="AC16UZ_05830"
     CDS             complement(3458..>3924)
                     /locus_tag="AC16UZ_05830"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6246238.1"
                     /translation="YADGYAAYEALPATLRDEISELTAIHSARRPYSHQGFLAGRGPE
                     RSMTILPSDEALQTQDHPLVRTHPETGRKALWVNGVYTIGIRNMAEEDAKNLLKRLFD
                     HAIRDEFVYRHRWAPDMLTMWDNRSVQHCARGGYDGYRRVMHRTTVAGDSPV"
BASE COUNT          640 a         1229 c         1361 g          694 t
ORIGIN      
        1 tccgcggatc tgatcgccac catcgagggg ttcagccggg aagacgtgga cggctacgcg
       61 gtggagagcc agacccgtgc cgctcgctcc attgccgagg gtcgattcga gaaggccctg
      121 atccccatct tcaacgacga cggttcgccc gcgctggacc gggacgagca tccccggcct
      181 gggacgacgc tggagagcct ggctgatctg aagccctcgt ttgccgccat gggctctcag
      241 caggtggaag gatttcccaa gccgttcgac gagatggccc gcgaccgcta ccctcaggtg
      301 gaggagatca accacgtgca ccacgccggg aactcctcgg gcgtggttga cggcgccagc
      361 gccgtgctcg tcgtgagccc ggattttgca aaggctcacg gtctgcgacc ccgagcccgc
      421 atccgcatga cggccaccgc cggaaccgag ccgctcatca tgttgaccgc accggctcct
      481 gcggcccagc gttgcctgca gaaggcgggc atgacgcggg acgacatcga tctgtgggaa
      541 atcaacgagg cgtttgcttc cgtcgtgctc aagttcatga aggagatggg ggtcgatcac
      601 gacgtggtca acatagacgg tggcgccatc gcgttgggtc atcccatcgg cgcgacgggc
      661 ggcatgctca tcggcacggc ggtggatcag ctcgagcgcg aggacaagga aaccgcgctg
      721 gttgcgatgt gcacaggcgg aggaatgggt acggctacga tcctcgagcg gatctgaggt
      781 cggctccggc ggggtatccc aaggcgccgc tcagcgcggc gcgtgcccgc cgatccacac
      841 cgggctagac cacgccaggc tgtcgtcggc ctggcggacg cgcacgtagt agtagtcacc
      901 ctggcgcccg ctgacgtcgt cgatggtgaa agcttcgtcc atgcttccct gggtgatgat
      961 ccggcgaagg gtgatggtgt cctcccgtgg accggcgggc agctgcgccc gggtctcgcc
     1021 gttctcgaaa tctcttatct ccagcttcac ctccgcggcc ggcagctgct catggcggcc
     1081 gtacgtggcc ggcgcgccgc cggtttcctg ggtgggttcc agggtaacgc ggacctgcgt
     1141 ggctctggat acgtcctcca ggtcgagccg aatgctgctc gtgtccccgc gggtgatggt
     1201 cgagaaacga agcaggtccg gatcccctgc gtccgcttcg atctgctgga gaagctgatt
     1261 gttgaaatcc atgccgctgg catcgatgag cgtcgcgtcc ctcacctcga tggttccacg
     1321 ccagtggcgc caccctcgcg gattgtctcc cggctgtcgc ggatcggagt cggaggcgaa
     1381 ggtcagcagg aaagctcccg gagagctcgt attgtcggca gcgtcggtca gcagttcccg
     1441 gcgccagatc tcctcgccgt ttcgcagcaa cgagatgtcg tcgattggcg ccgtgccgat
     1501 cacccgacct tcgatcgtgc gggtttcgga gaagggtccg cgctgcccca tgggcgtccc
     1561 gttcaccgag aactccagga tgatgcgctc gccggaagtc gcataggcgc gagcctgctt
     1621 catggcgtcg aacagcgcat cggtggtcct ggcccgggca atgacggcgc ccagaccgcc
     1681 tcgctgggca agtgtcccgt tcttgggggc accgtatccc ggctggctga gatggttgtc
     1741 tgacgcggcg gtgaagccca cctggtgccc gtggcgcaga taggcctgac cgaaccattc
     1801 gaagttgccg tgctgggagt tgacctccac gaggtgctcg agttccgggt cgctggtgcg
     1861 gtagtcgccg gcctggtgcg cgtgggggat gacgagcacg tcctctggct gggcatcacc
     1921 gcgcaggcct gcgtaaagct ttgacaggga cgggtagaag ggcgctgcca gccgattgcg
     1981 gccggcgggc gtgcggaaca gcacgttgtg gtgtccgccg tgggtattcc ggaccgtcca
     2041 ctcgtagccg aggaaggcga cgaactcgcc ttcccgggtg tagctgcggg cgttgttgcg
     2101 cagctgctcc cactcgccat cgtccagcca gatgtcgtgc tccgaatggg tgacgaagtc
     2161 caggcgcgca tcgtcgcgcg cccagcgcat gaagaagtcg ggcgtgccga taccctccgc
     2221 gaatcccgag tggccgtggg tatcgcccca gaatatccgc tcctcggccg tctgcttgac
     2281 caggattgga tttccacgtc cgctgatctg gccgtcccgg ctcgcgatgg tgaaccgata
     2341 tacgcctggt tcggctatct cgatgttgcg caacagggtg atcggctcct cgctggccgg
     2401 cacctcccgg aacggttcgc cattgaggga aaccagccag tccggatgtc cgtccgaggc
     2461 gcggttgtag aagcggtcct cggcccggac cgccagggtg aagggctctc cggccttcac
     2521 gatagacggc gcgaaggcgt gcacgccggc cgccgggccg ccgatcaccg ccacgggttg
     2581 aatgggtagc gagaagaagc gtccggatcc ggtgaacgcc acgtacaggg gcaacggcat
     2641 catgtcggag gaaaaatcgg ccatcaggaa tccgcggctc ccctggctgc ggtcaccgta
     2701 ggtcagggtc acggtatcgc ccgggcgcag cgtcccggag gcaacgcgaa agacgagcgc
     2761 gtcagccgga cggcggaaac cgccgtgcat gccgatcatc ggggcggttt cggtggtgaa
     2821 actcgcgtcg gcagcgctgg tggttattga caggtagttg tccgcggttg gatcgcccgt
     2881 ctgccacgcg ccgtagttgg gctggaaatg cctggccacc agaatgcctc cgcccgcctg
     2941 tatcgccttg gaaccgacgg tgaacgtctg cctcagcgtg acgaaggacg ctgcctggaa
     3001 gggtcccgaa tcggctcgaa gggttccaag cgccccgggc tcctcgtcga gcagggtcaa
     3061 ctcctggatg aagccgtcga tgacgtcgtg ccgatcggcc atcaggtccg gataggcgaa
     3121 cacgaaagag acggcctggg tcaggttgac cggcagcaca ccaccgtcga gagacaaggc
     3181 attggcccag ttccaaagca cgcgggatcg ctccgcgacg ttggtggtgt tggtcggtac
     3241 ctgctggacg tcggccttca gccgttcgac gcgcgcgcgc agatcgggcg tcaggtaatc
     3301 cgtggtgggc tgcgcgaaag ccgacagggc gagcagcatg agcaggggcg cggtgaacga
     3361 actaaaggcg cgcaagcgtc agggctccac ggtcactgga ccggggcgag cttctcacat
     3421 ggcggcgtgg gagggtagcg caatcgccgg ccgggcctca gaccggcgag tcgccggcca
     3481 cggtggtgcg gtgcatgacc cggcgatacc cgtcgtatcc gcccctggcg caatgctgaa
     3541 ccgagcggtt gtcccacatg gtcagcatgt cgggcgccca gcggtgacgg tagacgaact
     3601 cgtccctaat ggcgtgatcg aacaaccgct tcagcagatt ctttgcgtct tcctccgcca
     3661 tgttccgaat gccgatggtg tagacgccgt tcacccacag ggccttgcgg ccggtttcgg
     3721 ggtgcgtgcg aacgagcgga tggtcctggg tctgcagtgc ttcgtcgctg ggcaggatgg
     3781 tcatgctccg ctccgggcct ctcccggcca ggaatccctg gtggctgtag gggcggcggg
     3841 cgctgtggat cgcggtgagc tcggagatct cgtcgcgcag ggttgccggc agggcctcgt
     3901 aagccgcgta gccatccgcg tacc
//
LOCUS       DBNHOX010000264         3083 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_536145, whole genome shotgun sequence.
ACCESSION   DBNHOX010000264 DBNHOX010000000
VERSION     DBNHOX010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_536145"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="AC16UZ_05835"
     CDS             <1..710
                     /locus_tag="AC16UZ_05835"
                     /inference="COORDINATES: protein motif:HMM:NF037144.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR6246239.1"
                     /translation="AGNDAFTYSVGDGRGGSDSATVTVTVTGMNDAPSAVNDAAATAS
                     DTPVSVAVLSNDTDVDGDALSVVGVGAAGNGTVSHDGSAVQYTPAAGFSGTDTFGYTV
                     SDGQATASATVTVTVSAPAPATPGSFAATDGANGTAALTWTDSANETGYEVERESLHR
                     KRGVWVGTSLVATPGQDATSHTDASGTGNYRYRIRAVNGAGASSWTPWQEVAVTETSG
                     SGGGGGGTKGGGNGRKK"
     gene            complement(717..2150)
                     /locus_tag="AC16UZ_05840"
     CDS             complement(717..2150)
                     /locus_tag="AC16UZ_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6246240.1"
                     /translation="MSRKRTGHRGSQPYSSDPQTERSLRHSIKDGVYFSAMTGGAESY
                     FSAFAIHLNASTTIIGLLASLPPLLASFMQMLSAWLGRRTGRRKPIIVAGALLQAALL
                     VPIAAVPLLWPDAAAVLLIPLVFVYLCGPNLGAPQWNSLIGDLLPEARRGRFFALRTR
                     LSSVASLAALVIAGLVLEAFDKSGDAYWGFVVIFLLAGAARCVSAYHLNAMTEPTRNV
                     AGLLAPDDLSVWRRVRHSSLLRFSLFFAAMQFAVAISGPYFTLYMLRDLALSYVEFMV
                     LSFVSVTIQFLTLNRWGRLADLFGNRLILITTGSIITIIPSLWLFSTHYGWLLVVQAV
                     SGLAWSGFTLSATAFVFDLTPSEHRATLFAVHNVLAAVAVFLGAGAGALIAAHMPCSL
                     SGFGMEVPLLTPLYGVFFVSCLMRLTVAALFLRTINEVRRVRPMSPGGLIFRVTRMHP
                     ISGLIYEIVGRSRPRSAAPTSRDPAER"
     gene            2438..>3083
                     /locus_tag="AC16UZ_05845"
     CDS             2438..>3083
                     /locus_tag="AC16UZ_05845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR6246241.1"
                     /translation="MLVAAIAATFAGSAALAGLVFWPAGTFAWPRAWGYLALVGANFV
                     VNLVYLQRVNPTLIAARARFGAGTKRWDVIWSVCFAPAFCSIYVVAGLDAVRYEWTAM
                     SDRLWWLGLAVFVPGTSLFSWSMGVNPFFEKTVRIQTERGHRVVDAGPYQIIRHPGYV
                     GFFGWCFSAPLLLGSWWAFVPATISALGMVVRTALEDRTLQRELPGYADYARRVR"
BASE COUNT          575 a         1010 c         1054 g          444 t
ORIGIN      
        1 tcgccggcaa cgacgccttt acctactcgg tcggcgacgg ccggggcggc tcggactccg
       61 caacggtgac cgtgaccgtg accgggatga acgacgcgcc gagcgcggtg aacgacgctg
      121 ccgccacggc gtccgacacc ccggtatccg tcgcggtgct gagcaacgac acggacgtcg
      181 acggcgacgc cttgagcgtg gtcggggtcg gagcggccgg caacggcacg gtcagccacg
      241 acggctccgc cgtgcagtac acgcccgcag ccgggttctc cggaacggat acgttcggct
      301 acaccgtcag cgacggtcag gcaaccgcat ccgccacggt gacggtcacc gtctccgccc
      361 cggcaccggc aacgccggga agcttcgccg ccacggacgg cgcgaacggc acggcagcgc
      421 tgacctggac cgacagtgcg aacgagaccg gctacgaggt ggaacgcgaa tcgctgcaca
      481 ggaagcgcgg cgtttgggtc ggcacctccc tggtggcaac gccggggcag gacgccacca
      541 gccacacgga cgcctcggga accggaaact accgctaccg gatacgggcg gttaacggcg
      601 ccggcgcttc ctcctggacc ccatggcagg aggtcgccgt tacggagacc agcggcagtg
      661 gtggcggagg tggcggcacc aagggcggcg gcaacggccg caagaagtag ccaccgtcag
      721 cgctcggcgg ggtccctgga cgtgggcgcc gccgagcgcg gccgggatcg gccgacaatc
      781 tcgtagatca accccgagat cggatgcatg cgggtgaccc ggaagatgag ccccccgggg
      841 gacatcggcc tcacccgccg aacctcgttg atggttcgca gaaacagcgc ggcaaccgtg
      901 aggcgcatca ggcacgatac gaagaagacg ccgtaaagcg gcgttagcag aggcacctcc
      961 ataccgaaac cgctcaggga acaaggcatg tgcgcggcga tcagggcgcc ggctcctgcc
     1021 ccgaggaaca ccgccaccgc agcaaggacg ttgtgcaccg cgaacagggt cgcgcggtgt
     1081 tcggatgggg tcaggtcgaa cacgaaagcg gtggcgctca gcgtgaaccc cgaccaggcg
     1141 agcccgctga ccgcctgaac aaccagaagc cacccgtagt gggtagaaaa caaccacagc
     1201 gacgggatga tggtgatgat gctgccggtg gtaatcagga tcagccggtt gccgaaaagg
     1261 tccgccagcc gcccccaacg gttcagggtg aggaactgga tcgtgaccga cacgaagctc
     1321 agcaccatga actcgacgta gctcagcgcc agatccctga gcatgtagag cgtgaagtac
     1381 ggccctgata tcgccaccgc gaactgcatc gcggcgaaga acagcgagaa acgcagcaga
     1441 ctagagtgcc ggacccgccg ccacaccgaa aggtcgtccg gcgccagaag ccccgccacg
     1501 ttgcgcgtcg gctctgtcat ggcattgaga tggtaggccg agacgcagcg cgcagcgccc
     1561 gcgagcagga agatcaccac gaacccccag taggcgtctc cggacttgtc gaacgcctcg
     1621 aggacgagac cggcgatcac cagcgcggcc agggaggcaa cgctggacaa ccgggtgcgc
     1681 agggcaaaga aacggccgcg gcgggcctcc ggaagcagat caccgatgag gctgttccat
     1741 tggggtgcgc cgaggtttgg tccacacagg tagacgaaca ccagaggaat cagcagcacc
     1801 gcggcggcat cgggccagag cagcgggacc gccgcgatgg gcacaagcag cgccgcctgc
     1861 aggagggcac ccgctacgat gatcggcttg cggcggccgg tgcggcggcc gagccaggcg
     1921 gaaagcatct gcatgaatga tgcgagcagc ggtggcagcg aggcgagcaa cccgatgatc
     1981 gtcgtgctgg cattgagatg aatggcgaat gccgagaagt agctctccgc ccccccggtc
     2041 atggcggaga agtaaacgcc gtccttgatg gaatggcgca gcgaccgctc ggtctgggga
     2101 tcggacgagt aaggttggct cccgcggtgg ccggttcttt ttcgtgacaa tgtccgcttc
     2161 ccgccaaccg gccctaaacc cggagctccc gggcgaacaa tgcgcgaacc atcacgatac
     2221 tctgaaactg gcgcggcgca gattacccca gcgctgctcg cgacaccatc atcgcgcgtc
     2281 ggcgggacgt cctcgcacgg gccgtcgact tccagggaga tgggtttggc tatggccggc
     2341 agctgggtta acgtcgggcc cgatcgatca ccggcttgat atcgagcgct tggcaaacca
     2401 tccagttcca gcagggtccc cagggcagat cgggaaagtg ctcgtcgccg cgatcgccgc
     2461 cacgttcgcc ggcagcgcgg cgctggcggg cctcgtcttc tggcctgccg gcaccttcgc
     2521 ctggccccgg gcgtggggct accttgcgct cgtgggcgcc aatttcgtcg tcaatctcgt
     2581 gtatctgcag cgcgtgaatc ccaccctgat cgcggcgcgc gcgcgtttcg gcgccggcac
     2641 aaaacgctgg gacgtgatct ggagcgtgtg cttcgcgcct gcgttctgct cgatctacgt
     2701 cgtcgcgggc ttggacgccg ttcgctacga gtggacggcc atgagcgacc ggctgtggtg
     2761 gctgggtctc gccgtgtttg tgccggggac gtcactgttc agctggtcaa tgggcgtcaa
     2821 ccctttcttc gagaagaccg tccggatcca gacggagcga ggccaccggg tcgtcgacgc
     2881 cggcccttac cagatcatcc ggcatcccgg ctacgtcggc ttcttcggct ggtgcttctc
     2941 ggcccccctg ctgctgggtt cctggtgggc gttcgttccc gccaccatct cagcactcgg
     3001 catggtggtg cgaacggccc tggaggaccg gacgctgcag cgggagcttc ccggctacgc
     3061 cgactacgcc cggcgcgtcc gtt
//
LOCUS       DBNHOX010000265         4375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_986710, whole genome shotgun sequence.
ACCESSION   DBNHOX010000265 DBNHOX010000000
VERSION     DBNHOX010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_986710"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="AC16UZ_05850"
     CDS             complement(<1..624)
                     /locus_tag="AC16UZ_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450 family protein"
                     /protein_id="HIR6246242.1"
                     /translation="MSLVTVDSYQAANAALKQKDLRQALYDEGAVIMREVLVNLHGDE
                     HRTRRNVETQVFRRDFFRYYEHEVFPRTLEETIAPFLAAGKMDIVDFGYRVMVNLTAD
                     FTGIDRPKKSPEETEQLIKMLRLFGRTATLAHSTEDREPIRRAALDALADFQRDYVDP
                     SVARRQALIASVNRGDLSEEDLPRDILTVLLKNQDTVQLADDVRVREM"
     gene            complement(621..1925)
                     /locus_tag="AC16UZ_05855"
     CDS             complement(621..1925)
                     /locus_tag="AC16UZ_05855"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase family protein"
                     /protein_id="HIR6246243.1"
                     /translation="MKTLLKNANIIDGLGNAPFAGDVLIDEELILAVGAADGEGATVI
                     DVAGKTVMPGLIDAHCHVTLDETHSNDEMFFHRREGLGALVAAYNVQKLLRAGVTGFL
                     DADCIFDIGVDLRDAIEAGIVPGPRMSTGGNALLTSVGGTAGNLIPAEGRVGYAKVVR
                     SRDEIVTEVRRQIKLGVDWIKVHVTGLVPRQKHRGEIQAWSFDELKTVCDTAHELGIP
                     VVGHCRNAKSIADSVRAGMDMILHATFMDDAALEAVIERKVPLVPTFTFQANLADFGD
                     LVGADPNLREIFRREIAESADMIRRAYEAGVPVLCGTETGFSLTPYGEWHYREMEVFV
                     RDMGFTPLEAIRAATSECGRAIGLQDRVGAVEAGRLADLLVVDGDPSKDVTVLGDKTK
                     IERVFLNGAQLDLPPLPARKPIPGWRVSAYSSSQLFWADAHK"
     gene            complement(1922..4345)
                     /locus_tag="AC16UZ_05860"
     CDS             complement(1922..4345)
                     /locus_tag="AC16UZ_05860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6246244.1"
                     /translation="MSTAKEPHDPAGGVSRPDEQAVPSALPRAFGASVKRKEDRRLIT
                     GRGNYTDDLLLPDQAFAAFVRSPHAHAAINSIDAEAAREMPGVVAVLTGEEFAASGLG
                     PLMCGWMIHSRDGEPMKVGEHPALASDKVRYVGDAVALVVADSLQAARDAAECVYVDY
                     DDLPAVADVVSAGSVDAPELHSGIAGNEAFDWEIGDADAVAAAFARAAHITRLRLINN
                     RLVPNAMEPRSLNAQFDVGTGQHTLHITSQNPHGLRMTLAAVIGIAPEHKIRVISEDV
                     GGGFGSKAFNYAEEVACLWAARETGRPVKWAADRSEAFLTDAHGRDHVTTAELALDAD
                     GRFLALKVHTQANVGAYLSTFGSLIPTYVYAPLLSGQYEIPAIHANVIARYTNTAPVD
                     AYRGAGRPEAGYVVERLVEEAARELGIDSAELRRRNFVRSFPYVTPVEMEYDTGDFQA
                     TLDQALQLIDRDGFPARRAASEEQGKLRGLGIGAYIEAAGIGPSAKLGKLGSGAGLWE
                     SAEVRVNPTGSVEVLTGCHSHGQSHETTYAQLVADRFGIAVEDVDIVHGDTDKVQFGM
                     GTFGSRSGPVGLSAIAISCDKVVAKAKLIAAQVLDVPTQSVSFADGEFSSPQSNQRLS
                     FAEVALNAYTAHHFDTTQIEPGLKEGCYFDPPNFNFPAGCHVCEVEIDRETGVVEIVD
                     FVAVDDFGTLVNPMIVEGQVHGGVAQGIGQALLENTVYDDGGQLLSGSFMDYCMPRAD
                     DLPDIRVGFTNTPSPSNPLGIKGCGEAGAIGAPPAVINAITDALGVRELDMPATSEKI
                     WRVIKEQAL"
BASE COUNT          703 a         1463 c         1435 g          774 t
ORIGIN      
        1 catctccctt acccgcacgt cgtcggccag ctgcacggtg tcctggttct tcaggagcac
       61 cgtgaggatg tcccggggca ggtcctcctc ggacagatcg cctcggttca cgctggcgat
      121 gagcgcctgg cgacgagcca ccgaagggtc aacgtagtcg cgctgaaaat ccgcgagcgc
      181 gtccagcgcg gcacggcgaa tcggttcgcg atcctcggta gagtgggcga gggtggccgt
      241 gcggccgaac aacctcagca tctttatgag ctgctcggtt tcctccggcg acttcttcgg
      301 ccggtcgatg ccggtgaagt ccgcggtgag gttgaccatg acgcggtagc cgaagtccac
      361 gatgtccatc tttccggcgg cgaggaaggg ggcgatggtc tcctcgaggg tgcgcggaaa
      421 aacctcgtgc tcgtagtagc ggaagaagtc gcgacggaag acctgggttt ccacgttgcg
      481 ccgggtcctg tgctcgtcgc cgtgcaggtt gacgagcacc tcgcgcatga tcacggcgcc
      541 ctcgtcgtag agcgcctgac gcagatcttt ctgtttcagc gccgcgttgg cggcttggta
      601 cgagtcgacg gtgaccaggc tcatttatgt gcgtccgccc agaaaagctg gcttgaggag
      661 taggcggaga cccgccagcc gggaatgggc ttgcgtgcgg gcaggggggg caggtccagc
      721 tgcgcaccgt tcaagaacac ccgttcgatt ttcgtcttgt cgccgaggac cgtcacgtcc
      781 ttggacgggt cgccgtcgac taccaggagg tcggccagcc gcccggcctc caccgcgccg
      841 acgcgatcct gcaggccgat ggcgcgaccg cactccgacg tggccgcgcg tatcgcctcg
      901 agcggggtga aacccatgtc gcggacgaac acctccattt cccggtagtg ccactcgccg
      961 taaggcgtca gcgagaaacc ggtttccgtg ccgcacagca cgggcacgcc ggcctcgtag
     1021 gcgcggcgga tcatgtcggc gctctcggcg atctcccgcc ggaagatctc tcgcaggttg
     1081 gggtccgccc ccaccagatc gccgaaatca gcgaggttgg cctggaacgt gaaagtcggc
     1141 accagcggga ccttccgctc gatgaccgcc tcgagcgcgg cgtcgtccat gaacgtggcg
     1201 tgcaggatca tgtccatgcc cgcccggacg gagtccgcga tgctcttcgc gttgcggcag
     1261 tggccgacca cgggtatgcc gagctcgtgg gcggtgtcgc agacggtctt cagctcgtcg
     1321 aaggaccagg cctgaatctc accgcggtgc ttctgccgcg gaaccagccc cgtcacgtga
     1381 accttgatcc agtccacgcc gagctttatc tggcggcgca cctcggtgac gatctcgtcg
     1441 cggctgcgca ctaccttggc gtagccgacg cggccctccg ccggaatcag attaccggcg
     1501 gtgccgccca cggacgtcag cagagcgttc ccgccggtcg acatccgcgg cccgggcacg
     1561 atgcccgcct cgatggcatc cctcaggtcc acgccgatat cgaagatgca gtccgcgtcg
     1621 aggaaaccgg tgaccccggc acggagcagc ttctgcacgt tgtacgcggc caccagggcg
     1681 ccgaggcctt cccggcggtg gaagaacatc tcgtcgttcg aatgggtctc gtccagcgtc
     1741 acgtggcagt gcgcgtcgat gaggcctggc atgacggtct tgcccgcgac gtcgatgacg
     1801 gtcgcccctt ccccgtcggc tgcgcccacc gccaagatga gctcttcgtc gatgagcacg
     1861 tcgccggcaa acggcgcgtt gccgagcccg tcgatgatgt tggcgttctt cagcagtgtc
     1921 ttcacagcgc ctgctccttg atcacccgcc agatcttctc ggacgtggca ggcatgtcaa
     1981 gctcccttac cccgagagcg tcggtgattg cgttgatgac ggccggtggc gctccgatgg
     2041 cgccggcctc gccgcagccc ttgatgccga gcggattcga aggcgagggt gtattcgtga
     2101 acccgacccg gatatccggc aggtcgtccg cgcgtggcat gcaatagtcc atgaaggacc
     2161 cggacagtaa ctggccgccg tcgtcgtaga cggtgttctc cagcagcgcc tggccgattc
     2221 cctgagcgac cccgccgtgc acctggcctt ccacgatcat cgggtttacc aacgtgccga
     2281 agtcgtccac ggcgacgaag tccacgatct ccaccacgcc ggtctcacga tcgatctcca
     2341 cctcgcagac gtgacaaccg gcgggaaagt tgaagttggg cgggtcgaag tagcaaccct
     2401 ctttcagccc cggctcgatc tgtgtggtgt cgaagtgatg ggccgtatac gcgttgaggg
     2461 cgacttccgc gaaggaaagg cgctgattcg actgggggct cgagaactct ccgtcagcga
     2521 agctcacgct ctgcgttgga acatccagga cctgggcggc gatgagcttg gctttggcaa
     2581 ccaccttatc gcaggagatg gcgatggcgg acaggcccac cggacccgaa cgcgagccga
     2641 aggtccccat cccgaactgc accttgtccg tatcgccgtg gacgatgtcc acgtcctcta
     2701 cggcgatgcc gaaccgatcg gccacgagtt gcgcataggt cgtctcgtgg ctctggccgt
     2761 ggctgtggca gcccgtcagc acctcgacgc tgccggtggg attcaccctc acctccgccg
     2821 actcccacag ccccgcgccc gatcccagct tgcccagctt ggccgaggga ccgatcccgg
     2881 ctgcctcgat gtaggcgccg atgcccaggc cgcgcagctt gccctgctct tcggatgcag
     2941 cccgccgggc aggaaaacca tcacggtcga tgagctgcag cgcctggtcc agggtcgcct
     3001 ggaagtcgcc ggtgtcgtac tccatctcca ccggggtgac gtaaggaaac gaccgcacga
     3061 agttgcggcg ccgcagctcg gcagaatcga tgccgagttc ccgtgccgcc tcctccacca
     3121 gccgctcgac cacgtagccc gcctccgggc ggccggcgcc gcggtaggcg tccacgggtg
     3181 ccgtattcgt gtagcgggcg atgacgttgg cgtgaattgc cgggatctcg tactgccccg
     3241 acaacaaggg ggcgtagacg taggtcggga tcagcgagcc gaacgtggac aggtaggcgc
     3301 cgacattggc ctgagtgtgc accttcaagg ccagaaaacg gccgtcggca tccagggcga
     3361 gctcggcggt ggtgacgtga tcgcggccgt gggcgtcggt cagaaacgcc tctgaccgat
     3421 cggccgccca cttcacgggc ctgccggtct cgcgggccgc ccaaaggcac gccacttctt
     3481 ccgcgtagtt gaatgctttg gatccgaagc cgccgcccac gtcctcggag atgaccctga
     3541 tcttgtgctc gggggcgatg ccgatcacgg ctgccagcgt cattctcagg ccgtgggggt
     3601 tctggctggt aatgtgcagc gtgtgctggc cggttccaac atcgaactgc gcgttgaggg
     3661 agcgcggctc catggcgttc ggcaccaggc ggttgtttat cagccgcagc cgggtgatgt
     3721 gggcagccct ggcgaaagcg gctgctaccg cgtcggcatc gcctatttcc cagtcgaacg
     3781 cttcgttgcc tgcaatcccc gaatgaagct ccggcgcatc gacggaccct gccgaaacca
     3841 cgtcggcgac cgccggcagg tcgtcgtagt cgacgtacac gcactcagcg gcgtcccgtg
     3901 cggcttgaag cgagtcggca accaccaggg cgaccgcgtc gccgacgtag cggaccttgt
     3961 ccgacgccag cgccgggtgc tcgcctacct tcatgggctc gccgtcgcgg gagtggatca
     4021 tccagccgca catcagcggg ccgagaccgc tggccgcgaa ttcctcgccc gtcagcacgg
     4081 cgaccacgcc gggcatctcc ctggccgctt ccgcgtcgat ggaattgata gcagcgtgag
     4141 cgtgggggga tcgcacgaag gcggcgaagg cctgatcggg caggagcagg tcgtcggtgt
     4201 agttcccgcg cccggtgatc aggcgccggt cttccttgcg cttcaccgac gcgccgaacg
     4261 cccgcggaag ggcgctcgga acggcttgct catcgggtcg cgataccccg cccgccgggt
     4321 cgtgcggctc cttcgcggtg ctcatctttg gctctcccca cccgcaaatc ccggt
//
LOCUS       DBNHOX010000266         2691 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1242789, whole genome shotgun sequence.
ACCESSION   DBNHOX010000266 DBNHOX010000000
VERSION     DBNHOX010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1242789"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="AC16UZ_05865"
     CDS             <1..648
                     /locus_tag="AC16UZ_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246245.1"
                     /translation="FDPMLVTGLGDFNAEEIERYNRLHLLPFNPAIGRDCEDRPDPQL
                     PGRSYTSCTTVRERPAHPYSELGDEVLLSLAEHDAAAALMLGRRVAGEDERLYWYLRA
                     AALSEKSGPLMALAEQRYRDSMDLRLVRGELRRVPRPDRIAVRVALDTVAGRMGDPRA
                     NPDRWRGVLRSLAADRAPTYLAQADALVAEFLGAMARTQREITGSVQIQEIIDNA"
     gene            641..1228
                     /locus_tag="AC16UZ_05870"
     CDS             641..1228
                     /locus_tag="AC16UZ_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246246.1"
                     /translation="MRRRLLMAAALVFPGMAVAEALRTECAGCETLRDFGNYGAAQLY
                     RAVGPMGPAVLNDRIWVVNPDTGRQVFVDLDTPTTLVYFFGAPIPVPDLLRTEINATW
                     GDGSVMETWILPNEVIGSMAEALISVDSAGTPPVNAELLDRLPGFGYGGGWYHAGSVG
                     SMTSTHYGAWSFLVRYDSYGRAVVTVYECAYASVC"
     gene            complement(1248..2570)
                     /locus_tag="AC16UZ_05875"
     CDS             complement(1248..2570)
                     /locus_tag="AC16UZ_05875"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6246247.1"
                     /translation="MPEKTPNHTLSPMKCPVALADVDLFGEGAQEHWYEAYDILHRDA
                     PVHKIPGEGFQPGTDAYILTKYDDISMVVKDPVRFPPTLSLGVDALIDSGVDPNDMEY
                     TNAMMISMMTLRPTIELWRAHRQELTDPWVGPGADRNTEMITRCADRLIDNWIDRGEV
                     EFISEFARPLPQLVMANVLGFPEEDIPRLAEWGNAQVAPFVYGKGHRNILTDEQIKEQ
                     FELLDGFKEYVFDKVQEKRKDPQDDMISFLTQVEYKALGRKLTDMEVNGVVYAMVIGG
                     LETTQYALEEQAQLLCEREGIFDAIRQDREKLRQFTEEAMRLRSPTQGLSTRITSQDE
                     VFQGVKVPKGSMLHLRWAAANIDEQEFECPYELQLDRKAASRHLTFSAGPRVCPGAGI
                     SRLEQIIAWNRLLDRIDSLEYAPDNTFLHQPGIMLGTLELHLRFTKAQ"
BASE COUNT          440 a          819 c          879 g          553 t
ORIGIN      
        1 ttcgatccga tgctggtgac gggtctcggt gacttcaacg ccgaagagat cgaacggtat
       61 aaccgccttc acctgctccc attcaatcca gccatcgggc gtgattgcga ggatcgtccc
      121 gacccgcagt tgcccgggcg ctcctacacc agttgcacaa ccgtccgcga acgacccgct
      181 cacccttaca gcgagctggg cgacgaggtg ctgcttagcc tcgccgagca cgatgctgcc
      241 gcagccctga tgcttggccg ccgcgtcgcc ggagaagacg agcgccttta ctggtacctg
      301 cgtgctgcag cgctgtcaga aaagagcggg ccgctcatgg ccctggccga gcagcgttac
      361 cgtgactcga tggaccttcg gctcgtgcga ggggagttgc gccgcgttcc ccgacccgac
      421 cgcatagccg ttcgcgttgc cctggacacg gtggctgggc gcatgggaga ccccagggcg
      481 aacccggacc ggtggcgagg ggtgctgcgc tcccttgccg ccgatagagc gccaacctat
      541 ctcgcccagg ccgatgccct ggtggccgag tttctcggcg ccatggcgcg aacccagcgc
      601 gagatcacgg gcagcgtgca gatccaggag atcatcgaca atgcgtagac gtctcctgat
      661 ggccgctgcg cttgtgttcc ctggaatggc tgtcgccgag gccctgcgca ccgaatgcgc
      721 gggatgcgaa acgctgcgcg acttcggtaa ctacggcgcg gcgcagctgt atcgcgccgt
      781 aggccccatg ggcccggccg ttctgaacga tcggatctgg gtggtgaatc ctgacactgg
      841 caggcaggtt tttgtggacc tggatacgcc cactacgctc gtttacttct ttggtgcacc
      901 catccccgtg ccggatctcc tgcgcacgga gatcaacgcc acctggggcg atggctccgt
      961 gatggagacc tggatcctgc ccaacgaggt gatcggttcc atggccgaag cgctgatttc
     1021 ggtagactcc gcggggacgc cgccggtcaa tgcggaactg ctggaccgcc tgccaggctt
     1081 cgggtacggg ggagggtggt atcacgcggg cagcgtcggg agcatgacga gtactcatta
     1141 cggcgcgtgg tctttcttgg tgcgctacga cagttatgga cgggccgtgg tgaccgtcta
     1201 cgagtgtgcc tacgcgtccg tttgctgaga gagcgcgtcc tctttgccta ctgggccttg
     1261 gtgaatctga ggtgcagttc gagggtgccc agcatgatgc ccggctggtg caggaacgtg
     1321 ttgtcaggcg cgtactccag gctgtcgata cgatccagca gacggttcca ggcgatgatc
     1381 tgctccagcc gggagatgcc cgcgccgggg cagacgcgtg ggcctgccga gaaggtgagg
     1441 tgccggctcg ccgccttgcg gtccagctgc agctcgtagg ggcactcgaa ctcttgctcg
     1501 tcgatgttgg cggcagccca acgaaggtga agcattgagc cttttggtac cttgacgccc
     1561 tgaaagactt cgtcctggct ggtaatgcgt gtggaaaggc cctgggtggg agagcgcagg
     1621 cgcatggctt cctcggtgaa ctgacgcagc ttttcccggt cctgcctgat ggcgtcgaag
     1681 atgccctcgc gctcgcacag cagctgggcc tgctcttcca acgcgtactg ggtggtctcc
     1741 agcccgccga tcaccatggc gtaaacgacc ccgttgacct ccatgtcggt aagcttgcgg
     1801 ccgagcgctt tgtactccac ctgggtgagg aaggagatca tgtcgtcctg gggatccttt
     1861 cgtttctcct gaactttatc gaagacgtac tctttgaagc cgtcgagcag ttcgaactgc
     1921 tccttgatct gctcgtccgt caggatgttg cggtggcctt tgccgtaaac gaagggagcg
     1981 acctgggcat taccccattc cgccaggcgg gggatgtcct cctctgggaa tccgagcacg
     2041 ttggccatca ccaactgggg aagggggcgg gcgaactcgg agatgaactc cacctcgccc
     2101 ctgtcgatcc agttgtcgat caggcggtcg gcgcagcggg tgatcatctc cgtgttccgg
     2161 tctgcccccg gtcccaccca cgggtccgtc aactcctgcc gatgagcccg ccacagctcg
     2221 atggtcggcc gcagcgtcat catcgaaatc atcatggcgt tggtgtattc catgtcattc
     2281 gggtcaacgc ctgagtcgat gagcgcgtcg acgcctagcg acagggtcgg cgggaaccgc
     2341 accgggtcct tgaccaccat ggagatgtcg tcgtacttgg tcaggatgta ggcatccgtg
     2401 ccgggctgga agccctcgcc aggaatctta tggacgggcg cgtcccggtg caggatgtcg
     2461 taggcctcgt accagtgttc ctgggcgcct tcgccaaaca ggtccacgtc tgccagggcg
     2521 accgggcatt tcatggggct cagggtgtgg ttgggtgtct tttcaggcat gtcgtgtcgt
     2581 tcgtttttct agggaaacgc tgtgtccagg gacgctcagc atagcgtgag cggagggtca
     2641 aatcgatcgt ctgtcattcc cgccggtgcg ggcgtatatt cacgcggtca c
//
LOCUS       DBNHOX010000267         3974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1308005, whole genome shotgun sequence.
ACCESSION   DBNHOX010000267 DBNHOX010000000
VERSION     DBNHOX010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1308005"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="AC16UZ_05880"
     CDS             <1..242
                     /locus_tag="AC16UZ_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR6246248.1"
                     /translation="PTKKDYELGDEVFMLLNLMDEAEKIPVAGKVVWVTPSGAQGNRA
                     AGIGVQFNDQDDVARSKIETYLAGALQSDRHTHTM"
     gene            249..1010
                     /locus_tag="AC16UZ_05885"
     CDS             249..1010
                     /locus_tag="AC16UZ_05885"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009406818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR6246249.1"
                     /translation="MLVDSHCHLNYLDDPDSRLQAARLAGVDGFLCIGVDKDGIAAVL
                     DIAERHLDVWASVGQHPEAAESPPDWVSDYLTHPRVVAVGETGLDYHHGRQAHIQRLQ
                     RERFAWHLEIAGVAGLPVVVHTRDAVADTADLLRAHRDVGGVLHCFTETWDLAKSALD
                     LGYYISISGIVTFRNGENVREVASRVPRDRLLVETDCPWLAPAPHRGRTNEPAFVRDT
                     ATFLADHLNWDRDALAAQTSENFFRLFACGPQTAA"
     gene            complement(1007..1474)
                     /locus_tag="AC16UZ_05890"
     CDS             complement(1007..1474)
                     /locus_tag="AC16UZ_05890"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6246250.1"
                     /translation="MSERTTNVSVRSAGSGDASLLIEFNLAMAMESEDKGLDRATLAA
                     GVEHLIEHPLDGFYLVAELAGVPAGSLMVTYEWSDWRAGRFWWIQSVYVKPEYRRKGV
                     YSALHQSVRERALSAPDTCGIRLYVERENAGAQATYASLGMSETAYRLYEEEF"
     gene            complement(1471..3597)
                     /locus_tag="AC16UZ_05895"
     CDS             complement(1471..3597)
                     /locus_tag="AC16UZ_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6246251.1"
                     /translation="MKLTVERLFSDPPLTGSVPAAGQFSPDGRRAAFLRTAEDDRERQ
                     DLWRLDCGNGDLACWLDARQIDDQGSSPTAAEKAERERRRQFQGGISGFAFSPGGDKV
                     LVTAAGAGYLMDTANGRVDRFTPAGTRQTDLRFSPDGRHITYVRDGNLLCWDLAASSE
                     RAITNDGGGTVSYGAAEFIAQEEMHRFDGYWWSPDGTRIAYTRVDESPIPVTNRLEID
                     ADQIRTVEQRYPFTGGANAAVALLVMDWASGAVTEISYLQTPSDYLARVNWAGSELAV
                     QTQSRDQRELRLRFFDPVSGNSRDGLEERSATWINLHDNFRYLEGERFLWTSERDGHS
                     HLYLCDGSRELPLTRGEGRVNRVLHADEHRILFTGWFGNPQEQHLYSVSASAASGPEE
                     PVRLTAEPGWHEVTVDLRGEQFLDRFSAVDRQPSCRVRKLEGPQVNTIGQELADGLSN
                     YAPYLTHHVTPQLGTVVCEDGQTLHYRLTRPSNGSRQKSPVIVHVYGGPGVQRVRNEW
                     APALLQLFVQHGFGVFELDNRGSGNRDRGFEEPIHRQLGKVEVADQVLGADFLRSLDW
                     VDPERIGVFGHSYGGYMTIMCLAQAPASFKAGVSVAPVTDWHLYDTHYTERYLGHPED
                     NASGYAASSVFPHLDGIKGRLLLIHGMADDNVLYTNSTMLYRALQARHFPFEMMAYPG
                     SKHSLQERDVSIHRFNLILDFFERAL"
     gene            complement(3594..>3974)
                     /locus_tag="AC16UZ_05900"
     CDS             complement(3594..>3974)
                     /locus_tag="AC16UZ_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR6246252.1"
                     /translation="SAEAAFELGITRTFLPHGLGHLLGIQTHDVGGQQTSPTGGLNAP
                     PDVYPALRLTRTVESGQVFTIEPGIYFIPKLLKELSTSEHAHVVNWDRVDAFLPHGGI
                     RIEDNVLVGTNGVENLTRNAFANL"
BASE COUNT          685 a         1275 c         1278 g          736 t
ORIGIN      
        1 ttcccaccaa gaaggactac gagctcggcg atgaggtttt catgcttctc aatctcatgg
       61 acgaagcaga aaagattccc gtggccggca aggtcgtctg ggtgacgccc tcgggcgcgc
      121 agggaaatcg cgcggccgga ataggcgtgc agttcaacga tcaggacgat gttgcccgct
      181 ccaagatcga aacctacctc gccggtgcgc tgcagtccga cagacacact cacaccatgt
      241 gaacggcctt gctggtcgat tctcactgtc acctgaacta tctcgatgac ccggactccc
      301 gtctgcaggc ggcgcggctt gccggcgtcg atgggtttct gtgcatcggg gtggataaag
      361 acggcatcgc cgccgtgctc gacattgccg agcgccacct tgatgtctgg gcgagcgtcg
      421 gacagcatcc tgaagccgcc gagagcccgc cggactgggt cagcgactac ctgactcatc
      481 cccgggtcgt cgccgtgggg gaaacgggcc tggactacca ccatggtcgc caggcgcata
      541 tccagcgcct gcagcgggaa cggtttgcct ggcatctcga gattgcgggc gtcgctggac
      601 tgcccgtcgt cgtgcatacc cgcgatgcgg tggcggacac ggccgacctc ctgcgggcgc
      661 atcgggacgt gggaggcgtt ctgcactgct ttacggagac ctgggacctc gccaagagcg
      721 cgctcgacct cgggtactac atctcgatat ccggcatcgt gacgtttcga aatggtgaga
      781 acgtccgcga ggtggcgagc agggtgcccc gtgaccgtct gctcgtagaa accgactgcc
      841 cgtggttggc gccggcaccg caccgtggcc gaaccaacga gcccgccttc gttcgtgaca
      901 cggcgacgtt tctcgcggac cacctgaact gggatcggga cgcgctggca gcgcagacca
      961 gcgaaaactt ctttcgcctc ttcgcctgtg ggccgcagac ggcggcctag aattcttcct
     1021 cgtaaagccg gtaggcggtc tctgacatgc cgaggctggc ataggttgcc tgggcgcctg
     1081 cgttttcccg ctcgacgtag agccggatgc cgcacgtgtc gggtgctgaa agcgcccgtt
     1141 cgcgcacgct ctgatgcagc gccgagtaga cgcccttgcg gcgatactcc ggtttcacgt
     1201 acacgctctg gatccaccag aatcgtcccg ctctccagtc gctccactcg taggtgacca
     1261 tcagggagcc ggccggcacc ccggcaagct ctgctaccag ataaaacccg tcgagcgggt
     1321 gctcgatgag gtgctccacg cccgcggcca gcgtcgcgcg atccaggccc ttgtcctcgc
     1381 tctccatcgc catggccagg ttgaactcga tcagcaggct tgcgtcgccg ctgcccgccg
     1441 accttacgct gacgttggtt gttctttcgc tcacagcgcc cgctcgaaga aatcgagaat
     1501 cagattgaaa cgatgaatgg agacatcgcg ctcctgaagt gaatgcttgg accccgggta
     1561 ggccatcatc tcaaagggga agtgccgggc ctgaagcgcc cggtagagca tggtgctgtt
     1621 cgtgtagaga acgttgtcat cggccatccc gtgaatgagc agcagcctgc ctttgatccc
     1681 gtcgagatgg ggaaatacgc tgctggcagc gtacccggaa gcattgtctt cggggtgccc
     1741 gagataacgt tccgtgtagt gagtgtcgta aagatgccag tcggtcaccg gtgcgaccga
     1801 aacgccggct ttgaacgacg caggggcctg ggccaggcac atgatggtca tgtagccccc
     1861 gtagctgtgc ccgaaaaccc caatgcgttc cggatcaacc cagtcgagcg agcgaaggaa
     1921 atcggcgccg agcacctggt ctgccacctc gaccttgccc agctggcggt gaatgggctc
     1981 ttcgaacccg cggtcgcggt tcccggaacc ccggttgtcc agctcgaaca cgccgaaccc
     2041 gtgctggacg aaaagctgca gcagcgcagg cgcccattcg ttacgcaccc gctgcacgcc
     2101 tggcccgccg tacacatgca cgatcaccgg actcttctgt cggctcccgt ttgatggtcg
     2161 cgtgagccgg taatgcaggg tctggccgtc ctcgcagacg acggtgccga gctgcggtgt
     2221 cacgtggtgg gtgaggtagg gcgcgtagtt cgaaagcccg tccgcgagct cctgcccgat
     2281 ggtgttcacc tggggcccct caagcttcct gacccgacag gacggctgcc ggtcgacggc
     2341 gctgaagcgg tcgagaaact gctcgcccct gaggtcgacg gtaacctcat gccagcctgg
     2401 ctccgccgtc aacctgacag gctcctccgg ccccgaagca gcggacgcgc tgaccgagta
     2461 gagatgctgc tcctgggggt tgccgaacca gccggtgaac aggatgcggt gctcatccgc
     2521 gtgaagcacg cggtttaccc ttccctcgcc gcgagtcagc ggcagctccc ggctcccgtc
     2581 acagaggtag agatggctgt ggccgtcgcg ctccgacgtc cagagaaagc gctcgccttc
     2641 gaggtagcgg aagttgtcgt gcaggtttat ccaggtggcc gatctttctt ccagcccgtc
     2701 tcgggagttc cccgataccg ggtcgaagaa gcgcaaccgc agttcccgtt gatcccggct
     2761 ctgtgtctgc accgccagct cgctcccggc ccagttcact ctcgccaggt agtcgctcgg
     2821 cgtctgcagg tacgaaattt cggtaaccgc gccggacgcc cagtccatta ccagcagcgc
     2881 gacggccgcg ttggcgccgc ctgtaaacgg atagcgctgc tccacggtgc ggatctgatc
     2941 ggcatcgatc tccaaccggt tggtgacggg gatgggcgac tcgtccactc gggtgtaggc
     3001 gatcctggtt ccgtcgggcg accaccagta gccgtcgaat cggtgcatct cctcctgggc
     3061 gatgaactct gccgctccgt aggagacggt gcccccgcca tcgttggtga tggcgcgctc
     3121 tgacgaagct gcgagatccc agcagagcag attgccgtct ctgacatagg tgatgtggcg
     3181 cccgtccggc gagaaacgca ggtccgtctg gcgagttccg gcaggcgtga accgatcgac
     3241 gcggccgttg gccgtatcca tcagatagcc ggcgccggca gccgtgacca gcaccttgtc
     3301 accgccgggg ctgaacgcaa agccggagat cccgccctga aactggcggc ggcgttcgcg
     3361 ttcggctttt tcggccgccg tcgggctcga tccctggtcg tcaatctgtc ttgcatccag
     3421 ccagcaggcc aggtcaccgt tgccgcaatc caggcgccac aggtcctggc gctcgcgatc
     3481 gtcttcggcg gtgcgcagaa atgccgcccg gcggccgtca ggagagaact gacccgcggc
     3541 gggcacgctg cccgtcaacg gcggatcgct gaaaagccgc tcgacggtca gcttcataga
     3601 ttggcgaagg cgtttcgcgt caggttctcg actccgttcg tgcccaccaa cacgttgtct
     3661 tcgatgcgaa taccgccgtg gggcagaaag gcgtccacgc ggtcccagtt gacgacgtgg
     3721 gcgtgctcgc ttgtggagag ttccttcaac agcttgggaa tgaagtagat gcccggctcg
     3781 atggtgaaga cctggccgga ttcgacggtg cgtgtcaggc gcagcgccgg gtagacgtct
     3841 ggcggtgcgt tgagtccccc ggtcgggctg gtctgctggc cgcccacatc gtgggtctga
     3901 atcccgagca ggtggccgag gccgtgaggc agaaaggtcc gggtgatgcc cagctcgaag
     3961 gcggcctccg cgga
//
LOCUS       DBNHOX010000268         1776 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1115632, whole genome shotgun sequence.
ACCESSION   DBNHOX010000268 DBNHOX010000000
VERSION     DBNHOX010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1115632"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1257)
                     /locus_tag="AC16UZ_05905"
     CDS             complement(148..1257)
                     /locus_tag="AC16UZ_05905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6246253.1"
                     /translation="MHFGLSEEQTLLQDMLRRCLDEHLDIETLKRKAGQPCDPDLWGA
                     LVELGVPGVLIPEDHGGSGLGMMEAEVIAESMGRSVAPVPFLGTTVLAPLAWQLAGTP
                     EQQERHLGPIATGEHRYGVGLAEVAGAPRQFSNLTLKDDRAWGRRHFVLDANDASHFL
                     LTANGGTLIVIERDAEGVSIELLNDVDRTRSFAVVGLERTPFETVGAPGRAAQVIETL
                     LTAGRLMLAADTFGAAEIMLLRARDYSMERFQFDRPIGSFQGVKHLLADMVTDLEPCR
                     SLIWYAAHVFDAEPAERVLHACHAKSLASEVGKFIARSSIEIHGGMGFTEELGLHLWY
                     KRIEANRQLLGGPETVRHAAAVDQGWVEPLTTTVT"
     gene            complement(1303..>1776)
                     /locus_tag="AC16UZ_05910"
     CDS             complement(1303..>1776)
                     /locus_tag="AC16UZ_05910"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6246254.1"
                     /translation="PRINSFLSIIRGNQDIMGTLIFGGVFERYPDLKVVCVEADAGWV
                     PHYMYRMDHAYKRHRNWLAPGITLSKLPSEFFADHIYTTFQDDWVAFRSADQMNWRRL
                     MWANDFPHSDSTWPWSQEMLAEHTARLTDEQKRAILCDNVAELYGIDLRTLKAAA"
BASE COUNT          328 a          558 c          566 g          324 t
ORIGIN      
        1 gccagtacgc cggggcccgg acgctccgtg accccgggaa tgccgcggct gcgctgttct
       61 gccgggcgat ggcttcggca gaacttctgc acttcagcgc gaaagtcgtc gtattccggg
      121 ccgtagctca ggtccatgaa tgactcctca ggtaacggtg gtagtgagtg gttcgaccca
      181 gccctgatcg accgctgcag cgtggcggac ggtctcgggt cccccgagca gctggcggtt
      241 ggcttcgatg cgcttgtacc acagatgcag gcccagctct tcggtgaagc ccatcccacc
      301 gtgaatctca atcgaagagc gggcgatgaa cttgccgacc tcggatgcca gggacttggc
      361 gtggcaggcg tgcaggaccc gctccgccgg ctccgcgtcg aagacgtggg cagcgtacca
      421 gatcagtgat cggcagggct cgagatcggt gaccatgtcg gccaggagat gcttgacgcc
      481 ctggaacgag ccgatggggc gatcgaactg gaagcgctcc atcgagtaat cccgggctcg
      541 gagcagcatg atttcagcgg cgccaaaggt atcggccgcg agcatcagcc gccccgcggt
      601 gagcagagtc tcgatgacct gtgcggcacg ccccggcgca ccgactgtct cgaacggcgt
      661 gcgttcgaga ccgacgacgg caaagctgcg ggtacggtcg acgtcgttca gcaactcgat
      721 cgacacccct tccgcgtcgc gctcgatgac gatcagcgtg cctccgttgg cagtcagcag
      781 aaagtggctg gcgtcgtttg catcgagaac gaagtggcgg cgtccccagg cgcgatcgtc
      841 cttcagggtc aggttgctga actgacgtgg cgctccggca acctcagcga ggccgacccc
      901 gtagcgatgt tcgccggtgg cgatcggtcc gagatgtctc tcctgctgct cgggagtgcc
      961 ggcaagctgc caagccagtg gcgcaagcac cgtggtgccg agaaagggta caggcgccac
     1021 cgaccgtccc atggactcgg caatcacctc tgcctccatc atgcccagtc ccgagccacc
     1081 gtggtcctca ggaatcagca cccccgggac gccgagctcc accagcgctc cccacaggtc
     1141 cgggtcgcat ggctggccgg ccttgcgctt cagagtctcg atatcgagat gctcatccag
     1201 gcagcggcgc agcatgtcct ggagcaatgt ctgctcttca ctcagtccaa aatgcatcag
     1261 tcctccaaca ggcctttttc gactactgaa ccgggctctg actcaggctg cggccttcag
     1321 tgtgcgcaga tctatgccgt agagttcggc gacgttgtcg caaaggatgg cgcgcttctg
     1381 ctcgtcggta agtcgagctg tatgctcggc cagcatctcc tgcgaccagg gccaggtcga
     1441 atccgagtgg gggaagtcgt tggcccacat cagccggcgc cagttcatct gatcggcgga
     1501 gcgaaaagcc acccagtcgt cctggaacgt ggtgtagatg tgatcggcaa agaactcgct
     1561 cggcagcttc gacagcgtga tgccgggtgc gagccagttg cggtgccgct tgtaggcatg
     1621 atccatgcga tacatgtagt gcggaaccca gcctgcgtcc gcttccacgc agacgacctt
     1681 cagatcgggg taacgctcga acacaccccc gaagatcaga gtccccatga tgtcctggtt
     1741 accgcggatg atggacagaa agctgttgat acgcgg
//
LOCUS       DBNHOX010000269         3773 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_279694, whole genome shotgun sequence.
ACCESSION   DBNHOX010000269 DBNHOX010000000
VERSION     DBNHOX010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_279694"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="AC16UZ_05915"
     CDS             complement(<1..914)
                     /locus_tag="AC16UZ_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HIR6246255.1"
                     /translation="MLTALVLTAVLTAVVAALGAALVWADRRYPPDTRTLVESIDGLL
                     PQTQCAQCGYPGCRPYAEAVAAGAAINLCPPGGPETHRALARLLGRSEEDPPQEASKL
                     KARIREDGCIGCYLCVEACPVDAIVGAPRYLHTVLEAACTGCELCVPACPVDCIDMTP
                     AREASPISHQPRRRWPDRQSSPCIRCGACQPACPVGLSPQELLWYAGGSSSGRPTGQA
                     AAATRGLDRCIECGLCNPVCPSNIDLVGVFRQARRAQAARHEAERDAADAKARHARRT
                     ERLEETTAKRAEDREQRIARRSGRQWRA"
     gene            complement(934..1512)
                     /gene="rsxA"
                     /locus_tag="AC16UZ_05920"
     CDS             complement(934..1512)
                     /gene="rsxA"
                     /locus_tag="AC16UZ_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HIR6246256.1"
                     /translation="MAELALIVVGALLVNNFVLAQFLGLCPFIGVSGNYDTAVAMGFA
                     TTFVLTLAAAVSYMAYHWLLLPLGLEFLRVIVFIVVIAALVQLTEVFLRNTSPVLHNA
                     LGIYLPLITTNCAVLGVALLAINHSLSLLGTVAYAIGAAVGFTMVLVVFAAMRERLAH
                     AEVPAAFRGAPITLISAGILSLAFLGFAGLGN"
     gene            complement(1519..3549)
                     /gene="metG"
                     /locus_tag="AC16UZ_05925"
     CDS             complement(1519..3549)
                     /gene="metG"
                     /locus_tag="AC16UZ_05925"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR6246257.1"
                     /translation="MSSKPRKIMVSSALPYANGYIHMGHLVEYIQSDIWARFQKLRGN
                     ICTYVCAADAHGTPIMIKARQENLAPEAMVTRVAAEQHEDLKNFDVAFDNFHTTHSEE
                     NEYLTCRMFEALRDAGHIYTKTIEQAFDEQEKMFLPDRFVKGSCPRCESEDQYGDACE
                     NCGATYTPNDLLKPVSVLSGTTPIWKESEHYFFRLSAFEERLREWMKSADLHQNITSK
                     LEEWFEAGLQDWDISRDAPYFGFRIPGTEDKYFYVWLDAPVGYLASFLHYCNSHEGFD
                     FDEYWEPGHETEVYHFIGKDIVYFHTLFWPAVLEGAGFRKPTRLHVHGFVTVEGRKMS
                     KSRGTFVNASTYLEHLNPEFLRYYYATKLNGTIDDMDINLDDFVQRVNSDLVGKVVNI
                     ASRCAGFITKQFDGRLAGTLHDAELQGRFVDAAAPIAELFEAGDTSKAVREITALADQ
                     ANQYIAHHAPWSMIKEAGREAEVQAVCSQGINLFRILAIYLKPILPRMASEAEAFLEI
                     EPLAWKDVDAPLLDHPIGAFRPLFTRMERKAVDRMIEAGREAGSEPVPDSAPSSEAPA
                     ESQQISIDDFARIDLRVAKISAAEPVAGADKLLRLTLDLGDSNRQVFAGIKSAYEAQS
                     LQGRLCVVVANLAPRKMRFGVSEGMVLAAGPGGKDIFLLSPDSGAEPGMIVR"
BASE COUNT          640 a         1200 c         1255 g          678 t
ORIGIN      
        1 caggcccgcc attgcctgcc gctccgtcgg gcaatgcgct gctcgcgatc ctcagcgcgc
       61 ttggcggtcg tctcctcgag gcgttcggtg cgtcttgcat gccgcgcctt ggcatcggcg
      121 gcgtcccgtt cggcttcgtg ccttgcggcc tgcgctcgtc gcgcttggcg aaagacgccc
      181 acgaggtcga tgttgctggg acagaccggg ttgcaaagcc cgcactcgat gcagcgatcc
      241 aggccgcggg tcgcggcggc cgcctgaccg gtaggcctcc cgctggagga gcccccggcg
      301 taccagagca gctcctgggg gctcagcccc accgggcagg cgggctggca ggctccgcac
      361 ctgatgcatg gtgagctctg ccggtccggc catcgccggc gcggctgatg gctgatgggg
      421 gaagcctccc gggcgggcgt catgtcgatg cagtccaccg ggcacgccgg gacgcaaagc
      481 tcgcagcccg tgcacgccgc ctcgaggacc gtgtgaagat agcgcggcgc gcccacgatg
      541 gcgtccacgg ggcatgcctc cacgcacagg taacagccga tgcagccgtc ctctcggatg
      601 cgggctttga gcttggaagc ctcctgcggt gggtcttctt cgctgcggcc gagcaaccgg
      661 gcgagggccc ggtgtgtctc gggaccgcct ggagggcaca ggttgatggc cgcgcccgcg
      721 gcgacggcct cggcgtaggg tcggcagccg gggtatccgc actgcgcgca ctgggtctgg
      781 gggagcagac cgtcgatcga ctccaccagc gtccgcgtat ccggcgggta gcgccggtcc
      841 gcccagacga gcgcggcccc cagggcggcc accacggcgg tcagcacggc agtcagtacc
      901 aaagcggtca gcatggggga cctgcgctgc ccatcagttg ccgagccccg cgaacccgag
      961 gaacgcaaga gagagaattc ccgcgctgat cagggtaatg ggcgcaccgc gaaatgccgc
     1021 cggcacttcg gcgtgggcga ggcgttcccg catggccgcg aagaccacca gaaccatcgt
     1081 gaaacccacc gccgccccga tggcgtacgc cacggttccc agcagggaga ggctgtggtt
     1141 gatggccagc agggcgacgc ccagaaccgc gcagttcgtg gtgatcagcg gcaggtagat
     1201 gcccagggcg ttgtgcagaa cggggctcgt attgcgcaga aaaacctccg tcagctggac
     1261 gagggcagcg atgaccacga tgaacacgat gacccgcaga aactccagcc ccaaaggcag
     1321 cagcagccag tgataggcca tgtagctcac ggcggccgcc agggtcagca cgaaggtggt
     1381 ggcgaacccc atggccaccg ccgtgtcgta gttgccggac acgccgatga acgggcacag
     1441 gccgaggaac tgggccagca cgaaattgtt gacgagcaga gcgccgacga caatgagagc
     1501 gagctcagcc atggcggctc agcgcacgat catgcccggc tcggcgcccg agtccgggct
     1561 cagcaggaag atgtcctttc cgcccgggcc ggcggcgagc accatcccct cggatacccc
     1621 gaagcgcatc ttgcgcggag cgaggttggc aaccacgacg cacagcctgc cctgcagact
     1681 ctgtgcttcg taggcagact tgataccggc gaagacctgg cggttggaat cgccgagatc
     1741 cagagtcagc cgcagaagct tgtcggcgcc tgccacgggc tccgcggcgc tgatcttcgc
     1801 cactctcagg tcgatcctgg caaagtcgtc gatgctgatc tgctgtgatt cggcgggcgc
     1861 ctcgctggat ggggcggagt ccgggacggg ctcggagccc gcctcccggc ccgcctcgat
     1921 catccggtcc acggccttcc tctccattct cgtgaacaga ggcctgaacg cgccgatcgg
     1981 gtgatcgagc agaggcgcat ccacatcctt ccaggcgagc ggttcgatct cgaggaaggc
     2041 ttcggcttcg ctcgccatcc gcggcaggat cggcttgagg tagatcgcca ggatgcggaa
     2101 caggttgatg ccctggctgc acaccgcctg gacctcggct tctcggcccg cttccttgat
     2161 catggaccag ggcgcgtggt gggcgatgta ctggttcgcc tggtcggcca gcgccgtgat
     2221 ttccctcacg gccttgctgg tgtcgccggc ttcgaagagc tcggcgatgg gcgcggcggc
     2281 gtcgacgaac cgcccctgca gctcggcgtc gtgcagcgtg cccgccaggc gcccgtcgaa
     2341 ctgcttggtg atgaacccgg cacagcggct ggcgatgttg acgaccttac cgaccagatc
     2401 ggagtttacg cgctggacga agtcgtccag attgatgtcc atgtcgtcga tggttccgtt
     2461 cagcttggtc gcgtagtagt agcgcaggaa ctcggggttg aggtgctcga ggtaggtgga
     2521 cgcgttgacg aacgtgcctc gcgatttcga catcttgcgc ccttcgacgg tgacaaagcc
     2581 gtgaacgtgc agacgggtgg gcttgcggaa cccggcgccc tccagcaccg ccggccagaa
     2641 cagcgtatgg aaatagacga tgtccttgcc tatgaagtga tacacctcgg tttcgtgccc
     2701 cggttcccag tactcgtcga agtcgaatcc ctcgtgggag ttacagtaat gcaggaagct
     2761 ggcgaggtag cccaccgggg catcgagcca gacgtagaag tacttgtcct cggtaccggg
     2821 aatccggaat ccgaaatagg gcgcgtcgcg cgagatgtcc cagtcctgca ggcctgcctc
     2881 gaaccattcc tccagcttgc tggtaatgtt ctgatgaaga tccgccgact tcatccactc
     2941 tcgcagtcgc tcctcgaagg cgctcagacg aaagaagtaa tgctccgatt ccttccagat
     3001 cggggttgta cccgaaagta ccgagaccgg cttcagcaaa tcattcggcg tgtaggtcgc
     3061 gccgcagttt tcgcaggcgt ctccgtactg gtcctcggat tcgcatcgcg ggcacgagcc
     3121 cttcacgaag cggtccggca ggaacatttt ctcctgctcg tcgaaggcct gttcgatcgt
     3181 cttggtgtat atgtgcccgg cgtcgcggag cgcctcgaac atgcggcagg tcaggtattc
     3241 gttttcctcc gaatgcgtgg tgtgaaagtt atcgaaggcg acgtcgaagt tcttcaggtc
     3301 ctcgtgctgc tcggcggcga cgcgtgtcac catcgcctct ggcgccaggt tttcctgccg
     3361 cgccttgatc atgatcggcg tgccgtgcgc atcggccgcg cagacgtagg tgcagatatt
     3421 gccgcgaagc ttctggaacc gggcccagat gtccgactgg atgtattcga ccaggtgacc
     3481 catgtggata tagccgttgg cgtacggcag ggcactcgac accattatct tgcgcggttt
     3541 ggaactcata ggcctgggtc aatgatctgg agcggcgaac gcggcgcatt atgccacgcc
     3601 gaggcgaagc gggggcgatt cagtgtgccg cgccggcgtc ggcggcggcc tgttccagga
     3661 tccgctcctc ctcgacgagt atcttgaact tttgcttgtt gttggcctgc tggtatgcct
     3721 cgacggccga gaccttgcct ttctgcacaa gctccatgag cgccgaatcc atc
//
LOCUS       DBNHOX010000270         2056 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1501065, whole genome shotgun sequence.
ACCESSION   DBNHOX010000270 DBNHOX010000000
VERSION     DBNHOX010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1501065"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="AC16UZ_05930"
     CDS             <1..959
                     /locus_tag="AC16UZ_05930"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6246258.1"
                     /translation="SQLISSRPLGKIVSVTVWVYVALLVLGLTDDAAVLLEVVGFEVG
                     NFRLSLLLIVKMVVFVGVLLWVAIGLGNFIDSRLQGSEELTPSLAVLIGKVAKIVLIV
                     TATLIGLSGLGIDLTVFAVFSGAIGVGLGFGLQKVVSNFISGIIILMDRSIKPGDTIT
                     LGETFGWVRELRARFVSVVTRDGKEYLIPNEDFITHEVINWSFTDSLVRLDVEFGVSY
                     DSNPHEVSALAIEAAAACPRVYGRKTPVCWMTGFGDSSLDFVLRFWIDDPRQGLTNIR
                     GIVLLALWDAFSEHGIRIPYPHREILMKTPVVVARDEEPGEG"
     gene            1041..1796
                     /locus_tag="AC16UZ_05935"
     CDS             1041..1796
                     /locus_tag="AC16UZ_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246259.1"
                     /translation="MRTLNYLPCFLAVTMAMASPAFAQTAPGGGSQWHNTVEIYLMGP
                     NLDGSVGLGPVDGDVDVDPGTIFEALDGAFLGSYVGEAERWGVLADLAYMDLKAGGTG
                     PLGVVDYDVNVKQTIFGLVGLYRLTDTLQLTFGGRYVDVTNRLNLKGPGGERGLKASE
                     NWIDPTVGLRYVAPLGEKWVFNGMADIGGAGVGSDLTWVLSANIAYRITGRTQFYAGY
                     RYIYFDYEDGKGRDRFKFDMAQHGFLAGFRFEF"
BASE COUNT          344 a          618 c          654 g          440 t
ORIGIN      
        1 tgtcccagtt gatcagcagc cggccgctcg gcaagatcgt ctcggtcacc gtctgggttt
       61 acgtggccct gctggtgctg gggctgacgg acgatgccgc ggtcctgctc gaggtggtgg
      121 gcttcgaagt cggcaacttc aggctctcgt tgctgctgat cgtcaagatg gtggtctttg
      181 tcggcgtttt gctgtgggtt gccatcggcc tggggaactt catcgatagc cgcctgcagg
      241 ggtcggagga gctgaccccg tcgctggccg tgctgatcgg caaggtcgcg aagatcgtgc
      301 tgatcgtgac ggccacgctg atcggcctgt ccggcctcgg catcgacctg accgtgttcg
      361 cggtgttttc gggcgccatc ggcgtcggcc tgggcttcgg gctgcagaaa gtcgtctcca
      421 acttcatctc gggcatcatc atcctgatgg accgttccat caaacccggt gacaccatca
      481 ccctgggcga gacctttggt tgggttcgag agttgcgcgc ccgcttcgtt tccgtcgtga
      541 cccgagacgg caaggaatac ctgattccga atgaggactt catcacccac gaggtcatca
      601 actggtcctt tacggattcg ctggtgcgtc tcgacgtgga attcggcgtc tcctacgact
      661 ccaatccgca cgaggtcagc gcgctggcga tcgaggcggc cgctgcctgt ccccgcgtgt
      721 acggaaggaa gacgccggtc tgctggatga ccggattcgg cgattcttca ctggatttcg
      781 tcctgcggtt ctggattgac gatccacgcc aggggttgac gaatatccgc ggcatcgtgc
      841 tgctggccct ctgggacgcc ttcagtgaac acggcatccg catcccgtac ccgcaccgtg
      901 agatcctcat gaaaacgccg gtcgtcgtgg cccgggacga agagcccggc gagggataac
      961 cgtagccttc accccgccgc cggcagaaca gccaacggcg tataatgtga gtcctccctt
     1021 gacgattcca gagccctgca atgcgcactt tgaactatct gccttgcttc cttgccgtaa
     1081 cgatggccat ggcttccccc gcctttgcac agacggcgcc gggtggcggt tcccagtggc
     1141 acaacaccgt cgaaatctac ctgatgggcc cgaatctcga cggttcggtc ggcctcggcc
     1201 cggtggatgg cgacgtggac gtggaccccg gaacgatttt cgaggccctc gatggcgcct
     1261 ttctgggcag ctacgtcggt gaagcggagc gctggggcgt gcttgccgac ctggcttaca
     1321 tggacctcaa ggcggggggc acgggcccgc tgggcgtggt cgactacgac gtgaacgtca
     1381 agcagacgat tttcggactg gtcggcctgt accggctgac cgatacgctg cagttgacct
     1441 tcggcggccg ctacgtggac gtgaccaacc ggctgaacct gaaggggccg ggcggggagc
     1501 gtggcttgaa ggcctccgag aactggatcg acccgaccgt cggcctgcgt tacgtggcgc
     1561 cgctgggcga gaagtgggtt ttcaatggaa tggcggacat cgggggcgcc ggcgtcggtt
     1621 ccgatctcac ctgggtgctg tccgcaaaca tcgcctaccg gatcacgggg cgcacgcagt
     1681 tctacgccgg ctatcgttat atctatttcg attacgagga cggcaaaggg cgcgatcgct
     1741 tcaaattcga catggctcag catggcttcc tggcgggctt ccgtttcgag ttctgagtgc
     1801 gaaaaaagct cgcctgccgg gcaatatagt agacttatct aatgtagtaa aatctatatt
     1861 agtatatact catgttttta acgagagcca ggcccatgac cagcatattg tttgccgccc
     1921 caccgcatga tgcgggcggc ttggccaggc aattgccgtt aacgatgatc ctcggtgcga
     1981 ccctgatgct cgccttctcc ctggcgagcg cagccgggct gccggtcgcg ccggcggtac
     2041 ggtccgccgt gccgca
//
LOCUS       DBNHOX010000271         1857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_857646, whole genome shotgun sequence.
ACCESSION   DBNHOX010000271 DBNHOX010000000
VERSION     DBNHOX010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_857646"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1827
                     /locus_tag="AC16UZ_05940"
     CDS             <1..1827
                     /locus_tag="AC16UZ_05940"
                     /inference="COORDINATES: protein motif:HMM:NF013665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HIR6246260.1"
                     /translation="QHGTAIITGAAVLNALQIVGKKIEEVKLAASGAGAAGIACLDLL
                     VKLGMRRENILVCDRDGLIYKGRFEDEDSEKLPYARDTDMRTLAEAVVGADIFLGVSV
                     GGVLTRAMVSAMARRPIILALANPDPEILPEDAREAAPDAIIATGRSDYPNQVNNVLC
                     FPYIFRGALDAGATCINDEMQMACVRALAELTHREVQQKEAFSYQDEALVFGPDYIIP
                     RPFDPRLLASVAPAVAQAAMDSGVAARPIPDMAAYRQALDAFVYRSGMLMKPVFDAAR
                     RSPRRVVFAEGEEPVVLRAVQSIVDNGLAYPIVIGRPAVVEHRIEKFGLSIRPTVDFE
                     LVNPEQDDRYRDYWTTYHQLMQRRGVSPDIAKNVIRTRASVIAAIMVHKGEADAMLAG
                     VVGQFRTKLEHVLDVIGLAPGVKVPGSMSALTSDENTLFVCDTHVNADPDASQIAEVT
                     LMAAEKLRIFGMRPKVALLSHSAFGSEQDRLALKMRAALAILRERAPELEVEGEMTAD
                     MALDEDFRKQVFPNSKLSGRANLLVAPDIASAHISFNLARMLSNSVTVGPILLGVAKP
                     AHVLTSSATVRRVVNMTAIAVVDAQRLERDKAGTSVARGVVG"
BASE COUNT          345 a          570 c          628 g          314 t
ORIGIN      
        1 cagcacggaa ccgccatcat caccggcgcc gccgtgctga acgcgctgca gatcgtcggc
       61 aagaaaatcg aggaagtgaa gctggccgcc tcgggcgccg gggccgcggg catcgcctgc
      121 ctggacctgc tggtcaagct cggcatgcgc cgggagaaca tcctggtctg cgaccgggac
      181 gggctgatct acaagggccg cttcgaggac gaggattccg agaaactgcc gtatgccagg
      241 gatacggaca tgcggaccct ggcggaggcg gtggtcggcg ccgatatttt cctcggcgtc
      301 tcggtgggcg gtgtgctgac cagggcgatg gtgtctgcga tggcgcgcag gccgatcatc
      361 ctcgcgctgg ccaatccgga ccccgagatt ctgcccgagg acgcccgcga ggcggccccg
      421 gacgcgatta ttgccaccgg ccggtccgac taccccaacc aggtgaacaa cgtcctgtgt
      481 tttccataca tcttccgcgg cgccctcgat gccggggcaa cctgcatcaa cgacgaaatg
      541 cagatggcct gtgtccgcgc cctggctgaa ctgacccaca gggaagtgca gcagaaagag
      601 gctttttcat accaggacga agccctggtg ttcggcccgg actacatcat tccgcgaccc
      661 ttcgatccga ggctcctggc cagcgtcgca ccggcggtgg cgcaggcggc catggatagc
      721 ggtgtggcgg cccggcccat cccggacatg gccgcctacc gccaggccct ggatgcattc
      781 gtgtaccgga gcggcatgct gatgaagccg gtattcgatg ctgcgaggcg cagcccgagg
      841 cgtgtcgtct tcgccgaagg cgaggagccc gtggtcttgc gggcggtcca gtccatcgtc
      901 gacaacgggc tggcctaccc gatagtcatc ggccgcccgg cggtcgtgga gcaccggatc
      961 gagaagttcg gcctgagcat caggcccact gtcgatttcg aattggtcaa cccggagcaa
     1021 gacgaccgct accgcgacta ctggacgacc taccaccagc tcatgcagcg gcgcggggta
     1081 tccccggaca tcgccaagaa cgtcatccgg accagggctt cggtgattgc cgcgatcatg
     1141 gttcacaagg gcgaggcgga cgccatgctg gcgggcgtcg tgggccagtt ccggacgaag
     1201 ctggagcatg tactggacgt catcgggctg gcgcccggcg tcaaggtgcc gggctccatg
     1261 agcgcgctca cttcggatga gaatacgctt tttgtgtgcg acacccatgt caacgccgat
     1321 ccggacgcat cccagattgc cgaggtcacc ctgatggcgg cggagaaact caggattttc
     1381 ggcatgcggc ccaaagtggc gttgctctcg cactcggcgt tcggcagcga gcaggaccgc
     1441 ctcgcgctca agatgagagc ggccctcgcg atattgcggg aacgggcccc ggaactggag
     1501 gtggagggcg aaatgactgc tgacatggcg ctggacgaag atttccgcaa gcaggtattt
     1561 cccaattcga aactgagcgg ccgggccaac ctgctggtgg cgccggatat cgcttcggcc
     1621 catatcagtt tcaatctcgc gcgcatgctg agcaacagcg tgacggtcgg cccgatcctg
     1681 cttggtgtgg ccaagcccgc gcacgtgctg acgtcgtcag cgaccgtgag gcgggtcgtg
     1741 aacatgacgg cgatcgcggt cgtggatgcg cagcgtctcg aaagggacaa agcggggacg
     1801 agtgtcgccc ggggagtggt tggatgaacc agaaggacga aaaaatcttg caggatt
//
LOCUS       DBNHOX010000272        15369 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1308403, whole genome shotgun sequence.
ACCESSION   DBNHOX010000272 DBNHOX010000000
VERSION     DBNHOX010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 15369)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15369)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15369
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1308403"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="AC16UZ_05945"
     CDS             complement(<1..419)
                     /locus_tag="AC16UZ_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR6246261.1"
                     /translation="MDWQAKLNEGGIVLLDGGTGSELQRRGVPMSGAAWSGAAAHTHP
                     DVLRAVHHDYIAAGAEVIITNTFGTSRATLRAAGLGDAFASINRRAVALAMEARELAG
                     RDVAIGGSISNLPPNMNIGDYPDPAEELADLRDQAAIL"
     gene            514..1275
                     /locus_tag="AC16UZ_05950"
     CDS             514..1275
                     /locus_tag="AC16UZ_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246262.1"
                     /translation="MRTLVIQSASELDRPGWLSSCLQSVEGWARERGYDYRFRGDDAL
                     ELTPGWYRAKLIGRSPIVADLARLLLIRDALAMGYERACWIDADVLLYAPERLDLPDD
                     DECAFGREYWVQKNRDGRWQVRRNVHNALCSFVAGCPVLPFLIHATQRIISRADPQHI
                     APQMVGPKLLSALHNLTGFGLIDTIGALSPAVLDDVVQGGGEALRAMLAATPEPLAGV
                     NLCASVNGHRDLAALCRRLTDCSGLHLSGASHPAS"
     gene            1312..2631
                     /locus_tag="AC16UZ_05955"
     CDS             1312..2631
                     /locus_tag="AC16UZ_05955"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6246263.1"
                     /translation="MKEQSRYDHLREKAVLAPTTPTRRRQRIPFDPQLDPYEIPLQDL
                     NVANPELFRAEAFWPYFRRLREEAPVHYCSESQYGPYWSITRYDDIMQIEKNHAVFSS
                     EFSLGGVTLMGTPSSGNDVPMFIQMDPPKHDAQRKAVAPKFTQRNLGDLEATVRERAG
                     RILDGLPRNETFNWVEHVSIELTAQMLATLFGVPQEERHQLIRWSNIISNADNPEVVK
                     EKSSYFEALAQCGDYFDALWRDRKSSAPTGDLVSMLAHGPDTADMNSQELLGNAVLLT
                     VGGNDTTRNSISGGLLALNQFPEQYDKLLENADLVRTMVPEIIRWQTPLTHMRRTALA
                     DTEFRGQLIGKGDKVVMWYISGNRDPDAVERPDEFIIDRRRPREHLSFGFGIHRCLGN
                     RLAELQLRVLWEEILKRCPRIEVVGEPERASSVLFRAIQSLPVRIAA"
     gene            2704..3900
                     /locus_tag="AC16UZ_05960"
     CDS             2704..3900
                     /locus_tag="AC16UZ_05960"
                     /inference="COORDINATES: protein motif:HMM:NF047238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR6246264.1"
                     /translation="MAGQARSAAIVGIHEYPSRDVEGSVSPLQIKAESAARALADAGL
                     SWQDVDAIYDAGEAGMMSGLTIAEYLGLKPNVIDTTNVGGSSYEYHAAHAARDIAAGK
                     ARVALLTYGSTAHSNARAIGVGGRGGGGMHPADNMDAFAGMTLVANYAMVAQRHMYEY
                     GTTSEQLAEISVATRCHAMRNPEAVKAMEDLEFLDIRETTVEDVVGSRMIADPLHLLE
                     CCMISDGGGAVVIASPEVARNCARPPVWILGTGEATKYPENGGDITVSAAAQSGPQAF
                     AEAGVSPAEMDIAMIYDSFSITVLTILEDLGFCAKGEGGPWAAGGRLRFDRPADGPAL
                     NTDGGGLSSNHPGMRGIFLLVEAARQLRGDSCSQVDGAKLAVAHGNGGMLGSRHCAGT
                     VILGRD"
     gene            3912..4316
                     /locus_tag="AC16UZ_05965"
     CDS             3912..4316
                     /locus_tag="AC16UZ_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6246265.1"
                     /translation="MSEAKRPLPNLHEMDSRAFWEATREHELRYQECADCDTVVFYPR
                     RHCTGCLGDALAWKTAKGEGTVYTFSVIRQSYHPFFRALVPYAVAWIDLDEGPRLLSN
                     VIGPVEDIRVGQRVRVAWEDHEELAVPLFEPI"
     gene            complement(4370..4783)
                     /locus_tag="AC16UZ_05970"
     CDS             complement(4370..4783)
                     /locus_tag="AC16UZ_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6246266.1"
                     /translation="MTVNVIGDVEFGEELPVFEPDTSLANVKRFTEAAGWQGPRFTDH
                     EAARREGLPGALVPGIMSQGFLAAMIHRWAPESQIESVDTVFRAPVLVDERHSISGVV
                     TDIDEDAGTVEIDLTVTNEANETRVFGTATVRLPA"
     gene            complement(4780..5223)
                     /locus_tag="AC16UZ_05975"
     CDS             complement(4780..5223)
                     /locus_tag="AC16UZ_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAS1-like dehydratase domain-containing protein"
                     /protein_id="HIR6246267.1"
                     /translation="MLDLEALRANFLNEDFDEQHFEVNAEQCVAYALACGETAPRYTD
                     PAHPDFQAPPTLPSSFHPGRRLPDGFPKLPGLGMDAGKAVAPKRPIRPGVPLVGRTHL
                     HDVYAKTGRSGRMTFMVTRMEIYDPDGEQLATADTRIVIRERPDQ"
     gene            complement(5233..5862)
                     /locus_tag="AC16UZ_05980"
     CDS             complement(5233..5862)
                     /locus_tag="AC16UZ_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HIR6246268.1"
                     /translation="MILDLHTHSIKSDDGRAKVQNYCQWIRTREIPIDGLVLTEHRQF
                     DAESDYSPLAQQFGITILKGSEVETEYGHVLVFGVTEALTRAFDFADIHLPLARVIEA
                     CALHGAVPVPCHPGRRRVGMSAHLEEFGTPEGVRIVETHNGGSRGDEDAVAQAMAAEL
                     GYLGIGGSDAHIVSHVGRCATRFPGTISNEQELVEALLAEDFEAVTNRE"
     gene            complement(5943..7883)
                     /locus_tag="AC16UZ_05985"
     CDS             complement(5943..7883)
                     /locus_tag="AC16UZ_05985"
                     /inference="COORDINATES: protein motif:HMM:NF025114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6246269.1"
                     /translation="MGQLKFDHLQPIVDDDATIEAALADAHIPSLMAAMVHVSGDTSW
                     IRGAIRPVNQFFGDSQGGIAPNDQAEVRRRALEALIAYRDGKATPPPPDPATVSEMVN
                     FVIGQEVTSDYGEFLHAELALDGADPYASEGFDEISEAARREFKVIIVGAGMSGLLAA
                     IRLKEAGISWEIVEKHADVGGTWYQNGYPGCRVDSPNHIYSYSFRPADWPQYYSSQEV
                     LREYFDKVATEYGLRDQIRFSTEVQSMAFDDASGTWQVEVTNGTGARETLTANAVISA
                     VGQLNRPKWPDLAGQDRFRGVSFHSTEWEHEHDLSGKRIIVIGTGASAFQFAPEIAKV
                     AAEVEIFQRTPNWMAPVATYHDHIPLGKHWLLNHVPYYAKWYRFAMFWNMAEGVLSAV
                     RKDPAWNQTEDAISAENDTLRQMFTDWLEELCEGDEELLEKVVPKFPVGGKRVLLDNG
                     NWIRALRRDNVHLLTDPIAEINETGVVTEDGKQHDADVLIYGTGFQASRFLWPMKVTG
                     RDGIDLNEQWQGDARAYLGITIPNFPNLFCCYGPNTNIVVNGSIVFFSECEVRYILSC
                     IRQLLAGGHKALDCRADVHDAYNEWIDEGNANMAWGAARVPSWYKNEKGRVSQNWPFT
                     LLEYWNHTRDVNAADYEFLSDA"
     gene            8037..9122
                     /locus_tag="AC16UZ_05990"
     CDS             8037..9122
                     /locus_tag="AC16UZ_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6246270.1"
                     /translation="MDIGICVASHIGDLDYVVRAEALGYSHAWLADSQMLWSDCYATL
                     ALAADRTQKIRLGTGVAVSGTRPAPVNAAAIATINAIAPGRTFLGVGAGNTAMRVMGQ
                     PPHRIAEFDRYLSTLVPLVRGEEAQMGPLTVGARGEAPIRHIMPDKGFVNFDDRIPVY
                     VSGFGPRSLGLAGKHGDGAVLAMPANPAVMANIWRMLEEGAAAAGRTLDRSDFYTTGL
                     TAIVVLDPGEASDSPRVRAECGAMAMASVHYAYDQWRNFGHQPPGYLGGIWDDYCALL
                     ESYPQERRHQRIHAGHNCWVLPEEERFLTREVLETTCMIGTRDQLIDRLAALSEAGLD
                     QVMILPNFDTRFDVLERVGRELVGRIP"
     gene            complement(9184..10290)
                     /locus_tag="AC16UZ_05995"
     CDS             complement(9184..10290)
                     /locus_tag="AC16UZ_05995"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR6246271.1"
                     /translation="MIATFDNVDELGPEKVIEIYDPSTGLKAIVVVDNVAAGPAIGGV
                     RMAVDATREECGRLARAMTLKNAMAGLAHGGAKSVIVADPAMADEAKEVLIRSFAHAI
                     ADLRDYIPGPDMGTDETSMALVRDVTGRSAGLPRELGGIPLDEIGATGLGLSVSLEVA
                     EAFTDLSLDGARIVVQGFGSVGQHAARFLARRGARLVGVSDSRGARAAAEGFDLDLLL
                     ASKQEGVSVGAFDEGEAIDPEDLVELDCDVWIPAARPDVLNGANAGRIRAKVVAQGAN
                     IPATPEAEQTLAARGVLVIPDFVANAGGVICAAVEYHGGSESQALATIQDKIHGNVKE
                     VLSRATTTGELPRKVAMDIARERVQAAMRYRKTY"
     gene            complement(10287..11267)
                     /locus_tag="AC16UZ_06000"
     CDS             complement(10287..11267)
                     /locus_tag="AC16UZ_06000"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6246272.1"
                     /translation="MAEHTLLEAVNLALRRAMADDDRVLVLGEDVGVVGGVFRATDGL
                     LQAFGPHRVFDTPLAETGIAGVAVGLAVQGFRPVAEIQFMGFIYAAIDQLANHASRMR
                     TRTRGRQSCPLVLRAPFGGGIHAPEHHSESAEAMLAHIPGLRVVIPSSPRTAYGLLLA
                     AIRDPDPVVFLEPKRLYRSAKEAFEDDGAALPLDRCHIVRPGKDLTLVTWGAMVQETL
                     AAAEQLSGEGVAAEVIDVATIKPLDGETICESVGRTGRLVIVHEAPITGGFGGEIAAC
                     VAERALYSLQAPILRVAGYDTVMPLARLEHHYMPSVSRIVGAARETLEAS"
     gene            complement(11260..12336)
                     /gene="pdhA"
                     /locus_tag="AC16UZ_06005"
     CDS             complement(11260..12336)
                     /gene="pdhA"
                     /locus_tag="AC16UZ_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIR6246273.1"
                     /translation="MSDSLPFGVHPSTIIGPDGNLLRPIPEPLVDPATIRALYGWMHR
                     TRAFDAKAIELQRTGRLGTYASCLGQEAASVGIGHAMAPEDVLVPSFRDQGTQLYRGV
                     SMVELLLYWGGDERGSDYAVPRDDLPISITVGGHAPHAAGVALALKLRAEPRVAVCVF
                     GDGATSKGDVYEAMNIAGVWRLPVVFVVNNNQWAISTPRADQSAATTLAQKAVAAGFE
                     GSQVDGNDVLAVSEAVGDAIGQARKGDGPRLVECLTYRLGDHTTVDDASRYRSEAEVA
                     RWRQLDPLARVGAYLAQHEGWSDADEDALVERCDDEVASAATAYNGTTPQPPEAIFDH
                     LFASLPRELERQRATLLASAPADG"
     gene            complement(12362..14221)
                     /locus_tag="AC16UZ_06010"
     CDS             complement(12362..14221)
                     /locus_tag="AC16UZ_06010"
                     /inference="COORDINATES: protein motif:HMM:NF023282.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR6246274.1"
                     /translation="MTARLLIVFTLLIAGAASTRSAAAATFTGHVSVGYREVDVSGDE
                     GKYQQHVNLDDGARLFDLGMVFQPDRDANSPAGMPDRVELNASGLGGDPYESVRVRVQ
                     RYGSYRLTYDHRSSDYFYEDLLLAPEEASVEQSTGGDFHHFDFERNRDALALDLDLSS
                     RAKLNLGFARYEKRGESTTVLDVEREEFELDKPIDESLDRYELGFTYAWDHVTVALGQ
                     TWQEYDNDSQIFLPGFSPGSEADAPTSLDLFFLDQPYGFTSSDQRLSVQVRPSSQWIV
                     ELDARAGNLDLDMTATERAQGTDYLGRPFDREDAGAADVDRDTLLLDASVAYLVNDRL
                     QLNAGVRRYDLEQDGAVLFGDTAGASAWDIETTGFELGVDMAVTTTVTLSGGWSTERR
                     KSVVSHAIEGAGHTEAVDTDRDGFFGRLSYRPSRRAQVTLSFADDRIDDPYTLASATD
                     ARRYKARASYRWDNGLKVSASYRRTDYENDNTGWISESRQSDIRLTHAGDRLTVSVGA
                     AFVELQREVDQLVTGGSRQDLFAISYGADADFWDGAVRWHVTDRVDLTASYRAYENEG
                     SFRVDRDDVRAGVEFLLPADYALLLSYRNVDYNEDELESFDADVWELALKFRW"
     gene            complement(14229..15077)
                     /locus_tag="AC16UZ_06015"
     CDS             complement(14229..15077)
                     /locus_tag="AC16UZ_06015"
                     /inference="COORDINATES: protein motif:HMM:NF025890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR6246275.1"
                     /translation="MRLFSMAVGTLFLLATGQVGGNPTCAACHGELVADFHGTPHGAR
                     TEDDEASGCRSCHGDPATHLAAPAEPGSLLAFDDEPSATINAACGSCHADAHTARANP
                     HSQAGLACTDCHSIHPSSAAEIALPAEFDRVDAASATCFECHQETFSQFSFNERHRLQ
                     EGSVSCTSCHDPHAPASSGHLGAFGQRVCAECHADVDGPFVFEHDASRVEGCGACHEP
                     HGSPNRHMLTHQQVGELCYSCHAIVPQFHLGFSPAGPPRFDEGTVCTNCHATIHGSNL
                     DPAFLR"
     gene            15167..>15369
                     /locus_tag="AC16UZ_06020"
     CDS             15167..>15369
                     /locus_tag="AC16UZ_06020"
                     /inference="COORDINATES: protein motif:HMM:NF017689.7"
                     /note="uses the energy from reduction of ubiquinone-1 to
                     ubiquinol to move Na(+) ions from the cytoplasm to the
                     periplasm; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HIR6246276.1"
                     /translation="MLIEITTGLDVEVSGRPEQTIDTGPEVGQVALLGGDYLNLRPSL
                     EVDEGARVKAGDTIFTDRARPEIR"
BASE COUNT         2487 a         4987 c         5183 g         2712 t
ORIGIN      
        1 aggatggcag cctgatcgcg cagatcggcc agttcctcgg ccgggtcggg atagtccccg
       61 atgttcatgt tgggcgggag gttggatatc gacccgccga tggcgacgtc gcgaccggcg
      121 agctctctcg cttccatggc cagggccacg gcgcgccggt tgatggacgc gaacgcatcg
      181 cccaggccgg cggctcgaag ggtggcccgg gatgttccga aggtgttggt gatgatcacc
      241 tcagcgccgg ctgcaatgta gtcgtgatgc acggcgcgca gcacgtcggg atgggtgtgc
      301 gcggccgccc ccgaccaggc tgcgccgctc atgggcacgc ccctgcgctg gagctcggag
      361 ccggtgcctc cgtccagcag gacgatgccg ccctcgttga gcttcgcttg ccagtccact
      421 cgcttcacgt ttctaatgag acgaaaggat tttagctgac gtgagcgttt ccgtgccggc
      481 agcacccatc ggccgcgtgc gcaactgata cgcgtgcgga cacttgtcat ccagagcgcc
      541 agcgagctgg accggccggg ttggctgtcg tcgtgcctcc aaagcgtgga aggctgggcg
      601 cgggagcgtg gctacgacta tcgcttccgc ggcgacgatg cgctggagct cacgccgggg
      661 tggtatcgcg ccaagctcat cgggcgctct cccatcgtcg cggacctggc gaggctgctg
      721 ctgattcgcg acgcgttggc catgggctat gagcgtgcct gctggataga tgccgacgtg
      781 ctcctgtacg caccggaacg cctcgacctg cccgatgacg acgaatgcgc cttcggccgg
      841 gagtactggg tccagaagaa tcgcgatggg cggtggcagg tccgtcgcaa cgtgcacaac
      901 gccttgtgct ccttcgttgc cggctgcccc gttctgccct tcctgattca cgcgacccag
      961 aggatcatct cccgtgcgga tccccagcac atcgccccgc agatggtggg ccccaagctg
     1021 ctttccgccc tgcacaacct gaccggattc ggcctgatcg ataccatcgg tgctctgtct
     1081 cccgccgtcc tggacgacgt ggtgcagggc ggaggagaag cgctgcgagc catgctcgcg
     1141 gccacccccg agccccttgc aggcgtcaat ctctgcgcgt cggtgaacgg ccaccgggac
     1201 ctcgccgcgc tttgccgccg cctgaccgat tgcagcgggc tccatctctc gggcgcaagc
     1261 catccggcct cctgatatga tggcggggtc ggcgattggt cagcgaaggt tatgaaagag
     1321 caatctcggt acgatcatct gcgggaaaag gcggtgctgg cgccaacgac gcccacgcga
     1381 cgccggcagc ggattccctt cgatccgcag ctcgacccct acgagatccc gctccaggat
     1441 ctcaacgttg ccaaccccga gctgttcagg gcggaggcgt tctggcccta cttccgacgg
     1501 ctgcgggaag aggcgcccgt gcactactgt tccgaaagcc agtacggacc gtactggtcc
     1561 ataacccgct acgacgacat catgcagatc gagaagaacc acgcggtgtt ctcctccgag
     1621 ttctcgctgg gcggggtaac gctcatgggc acgccgtcct ccggcaacga cgtgccgatg
     1681 ttcatccaga tggatccgcc caaacacgac gcgcaacgca aagcggtagc cccgaagttc
     1741 actcagcgca accttggcga cctcgaggcc accgtgcggg agcgcgcggg caggattctc
     1801 gacggcctgc cccgcaacga gacgttcaac tgggtggagc acgtttccat cgagctgacc
     1861 gcgcagatgc tcgcaacgct gttcggcgtg ccccaggaag agcgccatca gctgatccgc
     1921 tggtcgaaca tcatcagcaa cgcggacaac cccgaggtgg tcaaggagaa gtcgtcgtat
     1981 ttcgaagccc tcgcgcagtg tggcgattac ttcgacgccc tctggcgcga ccgcaagtcg
     2041 tcagcgccca cgggcgatct ggtgtccatg ctggcccacg ggccggacac tgccgacatg
     2101 aattcccagg agctgctggg caacgcagtg ctgctgaccg tgggcggcaa cgacaccacc
     2161 cgcaactcca tcagcggcgg cctgctcgcc ctgaaccagt tcccggagca gtacgacaag
     2221 ctcctggaga acgccgatct cgtccgcacg atggtgccgg agatcatccg ctggcagacc
     2281 ccgctcacgc acatgcgccg cacggcgctg gcggacacgg agtttcgtgg ccagctgatc
     2341 ggcaagggtg acaaggtggt catgtggtac atatcgggga accgcgatcc ggacgccgtg
     2401 gagcggccgg acgagttcat catcgaccgc aggcggcccc gtgagcacct ttccttcggg
     2461 ttcggcatcc accgctgtct ggggaaccgt ctggccgagc ttcagctgag ggtgctctgg
     2521 gaggaaatcc tcaaacgatg ccctcgaatc gaggttgtcg gggagcctga gcgtgccagc
     2581 tcggtgctgt tccgcgccat tcagagcctg cccgtgcgca tcgccgccta gcccgcaagg
     2641 accggccttg gaccgtcggc ccgcacggct tagactgcgg gcatcaacct gggggagata
     2701 gccgtggctg gtcaagccag aagcgccgcc atcgtcggca ttcacgagta cccttcgcgc
     2761 gacgtcgagg gatccgtcag cccgttgcag atcaaggcgg aaagcgctgc ccgggcgctc
     2821 gccgatgccg ggctgagctg gcaggacgtt gacgccatct acgacgcggg tgaggccggg
     2881 atgatgagcg gtctcaccat cgccgagtac ctcgggctta aacccaacgt catcgacacc
     2941 accaatgtgg gcggcagctc ctacgagtac cacgccgccc acgcggccag ggacatcgcg
     3001 gcgggcaagg cgagggtggc gctgctgacc tacggatcga cagcgcacag caacgcacgg
     3061 gccatcggcg ttggcgggcg cggtgggggc ggcatgcatc cggcggacaa catggatgcc
     3121 tttgccggca tgaccctggt ggccaactac gcgatggtgg cgcagcggca catgtatgag
     3181 tacggcacca cgagcgagca gctggcagaa atatccgtgg cgacccgctg tcacgccatg
     3241 cgtaatcccg aggccgtcaa agccatggag gatctcgagt tcctcgacat tcgcgaaacg
     3301 acggtggagg acgtggtcgg ctcgaggatg atcgccgacc cgctgcatct gctggagtgc
     3361 tgcatgatct cggacggcgg cggagccgtg gtcatcgcct cgccggaagt cgcccgtaac
     3421 tgcgcgcgtc cgccggtgtg gatcctgggc accggagaag ccaccaaata ccctgaaaat
     3481 ggcggcgaca taacggtgag cgccgcggcc cagtccgggc cgcaggcctt tgctgaggcg
     3541 ggcgtctctc cggccgagat ggacatcgcc atgatctacg attcgttcag catcacggtg
     3601 ctcacgatcc tggaggatct gggattctgc gcgaaggggg agggcgggcc ctgggccgcg
     3661 ggagggcgtt tgcgcttcga tcgccccgcg gatggcccgg ccctgaacac cgatggcggc
     3721 gggctctcgt cgaaccatcc gggcatgcgg ggcatatttc ttctggtcga agccgcgcgc
     3781 cagctgcgcg gtgattcctg ctctcaggtg gacggtgcga agctggccgt ggcccacggc
     3841 aacggcggca tgctcggcag ccggcattgc gcgggtaccg tgatacttgg gcgggattga
     3901 ggagtagcac catgagcgaa gcgaagagac cgctgccgaa cctgcacgaa atggacagcc
     3961 gggcattctg ggaagccacc cgggaacacg agctgcgcta tcaggagtgc gcggactgcg
     4021 acaccgtcgt cttctacccg cggcggcatt gcacgggttg tcttggcgac gcgctggcct
     4081 ggaaaacggc caagggggag ggcaccgtct acacgttcag cgtgatcagg cagagctatc
     4141 accccttctt ccgcgctctg gtcccctacg cggtggcctg gatcgacctg gacgaggggc
     4201 cgcggctact gtccaacgtg atcggccccg tggaggacat tcgggtgggc caacgggtgc
     4261 gcgtcgcgtg ggaagaccac gaagagctgg ctgtgcccct tttcgagccg atctgaacga
     4321 ccggttgctg ccgcctttgc cggttgtgat cggcggatcc ggcgggagat caggcgggga
     4381 ggcgaacggt ggcggtgccg aagacccggg tctcgttggc ctcgttcgtc acggtcagat
     4441 caatttcgac cgtgcccgca tcttcgtcga tgtcggtgac gacgccgctg atgctgtgcc
     4501 tttcgtcaac cagcacgggc gccctgaaca cggtgtccac gctttcgatc tgactctccg
     4561 gcgcccagcg gtggatcatg gccgcgagaa aaccctgact catgatgcct ggcaccagcg
     4621 ctccggggag cccctcgcgc ctggcggcct catggtccgt gaaccggggt ccctgccagc
     4681 cggcggcttc ggtgaatcgc ttgacgttgg ccaggctcgt gtccggctcg aagaccggta
     4741 gctcttcgcc gaactcgacg tcgccgatga cgttgacggt cattgatccg gcctctctcg
     4801 aatgacgatt cgggtgtcag cggttgccag ctgctcgccg tcgggatcgt agatctccat
     4861 gcgggtaacc atgaacgtca tgcggcccga tcgtccggtt ttggcgtaaa cgtcgtggag
     4921 atgcgtgcgc ccgaccagcg gcaccccggg ccgtatgggc cgcttcggcg caacagcctt
     4981 gcctgcgtcc atgcccagac cgggtagttt gggaaagccg tcaggcaggc ggcggccggg
     5041 gtgaaagctc gacggcagcg tgggcggcgc ctggaagtca ggatgggccg ggtcggtgta
     5101 gcgcggcgcc gtctcgccgc aggccagggc gtaggcaacg cactgctcgg cgttcacctc
     5161 gaagtgctgc tcgtcgaagt cttcgttgag gaagtttgcc cgtaacgcct ctaggtcaag
     5221 catgggtgtc tttcactccc gattggtaac ggcttcgaag tcttcggcca gcagcgcttc
     5281 caccagttcc tgctcgttgg agatcgtgcc tgggaaacgg gtagcgcagc gtcccacgtg
     5341 gctgacgatg tgcgcgtcgc tgccgccaat gcccaggtag ccgagctcgg cggccatggc
     5401 ctgggccacg gcgtcctcgt ctccacggct gccgccattg tgcgtctcca cgatcctcac
     5461 cccttccggc gtgccgaatt cctcgagatg agcgctcatg cccacgcgcc ggcgcccggg
     5521 atgacaggga acgggaaccg cgccgtgcag cgcacaggct tcgatgaccc gggccaaagg
     5581 aaggtggatg tccgcgaaat cgaacgcccg ggtcagcgct tcggtcacgc cgaacaccag
     5641 gacgtggccg tactcggtct cgacctcgct gcccttgagg attgtgatcc cgaactgctg
     5701 agccagcggg gagtagtcgg actcggcgtc gaactggcga tgctcggtga gcacgaggcc
     5761 gtcgatgggg atctcgcgtg tgcggatcca ctggcagtag ttctgcacct tggcacgacc
     5821 atcatcggac ttgatggaat gtgtgtgcag atcgagaatc accgaacctc cttgaacgac
     5881 ccgaagcgcc gcgagcgcgc aatcttaacg tcgcgcgccg gcttctgcga gcgcggggcg
     5941 cgtcacgcgt cggagaggaa ctcgtagtcc gccgcgttga cgtccctggt gtggttccag
     6001 tactcgagca gcgtgaaggg ccagttctgg gacacccggc ctttctcgtt cttgtaccag
     6061 gagggtaccc gggccgcgcc ccaggccatg ttggcgttgc cctcgtcgat ccactcgttg
     6121 tacgcatcgt gaacgtctgc gcggcaatcc agggccttgt ggccgccggc cagcagctgg
     6181 cggatacagc tcaggatgta gcgcacctcg cattcggaga agaagacgat gctgccgttg
     6241 acgacgatgt tggtgttggg cccgtagcag cagaaaagat tggggaagtt ggggatggtg
     6301 atgccgaggt aggcgcgggc gtctccctgc cactgctcgt tcaggtcgat cccgtcgcga
     6361 ccggtgacct tcatcggcca tagaaaccgg cttgcctgga agccggtgcc gtagatcagc
     6421 acgtcggcgt cgtgctgttt gccatcctcg gtgacgacgc cggtctcgtt gatctcggcg
     6481 atgggatcgg tgagcaggtg cacgttgtcg cgcctcaagg cgcgaatcca gttgccgttg
     6541 tcgagcagca cccgcttgcc gcccaccggg aacttgggca ccaccttttc gagcagttcc
     6601 tcgtcgcctt cacacagctc ttcgagccag tcggtgaaca tctgccggag cgtgtcgttt
     6661 tcggcgctga tggcgtcttc agtctggttc caggccggat ccttgcgcac cgcggagagc
     6721 acgccctcgg ccatgttcca gaacatggcg aagcggtacc acttcgcgta gtagggaacg
     6781 tggttgagga gccagtgctt ccccagcggt atgtggtcgt ggtaggtggc caccggggcc
     6841 atccagttgg gtgtgcgctg aaaaatctcg acctcggctg ccaccttggc gatttcggga
     6901 gcgaactgaa acgcgctggc accggtgcct atgacgatga tgcgcttgcc ggacagatcg
     6961 tgctcgtgtt cccactcggt ggagtgaaag gacacgccgc gaaaacgatc ctggccggcg
     7021 aggtcgggcc atttcggtcg gttcagctgg cccacggcgc tgatgacggc gttcgcggtg
     7081 agggtctccc tggcgccggt accgttcgtg acttccacct gccacgtccc ggacgcatcg
     7141 tcgaacgcca tggactgcac ttccgtgctg aaacggatct ggtcgcgcag gccgtactcc
     7201 gtcgccacct tgtcgaaata ctcccgcaga acttcctggc tcgagtagta ctggggccag
     7261 tcggcgggcc ggaagctgta ggagtagatg tgattggggc tgtcgacccg acaccccgga
     7321 tagccgttct ggtaccaggt cccgcccacg tcggcgtgct tctccacgat ctcccaggag
     7381 atgccagcct ccttgagcct gatcgccgcg agcagccccg acatccctgc gccgacgatg
     7441 atgaccttga actcccgccg tgccgcctcg gagatctcgt cgaagccctc cgaagcgtag
     7501 gggtcggcgc cgtccagggc gagctcggcg tgcagaaact ccccgtagtc cgaggtgact
     7561 tcctggccga tgacgaagtt caccatctcg ctgacggttg cggggtcggg aggcgggggc
     7621 gtcgctttgc catcgcgata ggcaatcagc gcctcgagcg cccgtctgcg gacctctgcc
     7681 tgatcattgg gcgcgatgcc tccctgggag tcgccgaaga actggttgac cgggcgaatg
     7741 gctccgcgga tccagctggt atcaccgctg acgtgcacca tggcggccat cagcgacggg
     7801 atgtgggcat cggcgagggc ggcctcgatg gtcgcgtcgt cgtcgacgat gggctgcagg
     7861 tggtcgaact tgagctgccc catggctgat ttcccggctg gatatgccga tccatttagc
     7921 gcaggtagcc gttcggatgc aaggggacgg ctggggcgtc ccggccagga tcgtctggca
     7981 agcgctggcg cccttaagta cgctaatcgg ccaggttcac tatcccggag atcaggatgg
     8041 acatcggaat ctgcgtcgcc tctcacatcg gcgatctcga ctacgtcgtt cgcgccgagg
     8101 ccctcggata ttcccacgcc tggctcgccg acagccagat gctctggtcc gactgttacg
     8161 ccactctggc gctggccgcg gaccggaccc agaagatccg cctcggcacc ggcgtggccg
     8221 tctcgggcac ccggccggca cccgtcaacg cggcggccat tgcgaccatc aacgccatcg
     8281 cgcccgggcg gacgttcctc ggggtcggcg ccggcaacac ggcaatgcgc gtcatggggc
     8341 agccgcctca tcgaattgcc gagttcgacc ggtatctgag caccctcgtg ccgctggtcc
     8401 gtggcgagga ggcgcagatg ggacctttga ccgtcggcgc ccgcggcgag gcgcccattc
     8461 ggcacatcat gccggacaag gggttcgtca atttcgacga tcgaatcccc gtctacgttt
     8521 ccggcttcgg accgcggtct ctgggtctgg cgggcaagca cggcgacggc gcggtgctgg
     8581 ccatgccggc gaacccggcg gtgatggcca acatctggcg catgctggaa gagggcgcgg
     8641 cggcagcggg ccggacgctg gatcgatcag acttctacac gaccggactg accgccatcg
     8701 tcgtgctgga cccgggggaa gcgagcgatt cgccccgggt gcgagccgaa tgcggcgcca
     8761 tggccatggc ctccgttcac tacgcctacg atcagtggcg caatttcggt caccagccgc
     8821 ccggctatct tggcggcata tgggacgact actgcgctct gctcgaatct tatccgcagg
     8881 aaaggcgcca ccagcggatc catgccggcc acaactgctg ggtgttgccg gaagaggagc
     8941 ggtttctgac ccgcgaggtg ctcgagacca cctgcatgat aggcactcgc gaccagctca
     9001 tcgaccggct tgccgccctc agcgaagcgg gtctggatca ggtgatgatc ctgcccaatt
     9061 tcgatacccg gttcgacgtg ctcgagcggg tcggccggga gctggtgggc cgtattccct
     9121 gagccagggg cgcgctgggt cttcgccggg gcgaggcgct gccgtccggg gcatcactcc
     9181 gggtcagtag gttttacgat agcgcatggc cgcttgcacc cgttcgcgag caatgtccat
     9241 ggcgactttt ctcggcagct cgcccgtggt ggtcgccctg gagagcacct ctttgacgtt
     9301 gccgtgaatc ttgtcctgga tcgtggccag ggcctggctt tcactgccgc cgtgatactc
     9361 cacagccgcg cagatgaccc cgccggcgtt ggcgacgaag tccgggatca ccagaacgcc
     9421 gcgcgccgcc agggtctgct ccgcctccgg ggtggcgggg atgttcgctc cctgggcaac
     9481 taccttggct cggatccggc cggcgtttgc cccgttgagc acgtccggcc gggcggccgg
     9541 aatccacacg tcgcaatcga gctcgacgag atcctcggga tcgatggcct cgccttcgtc
     9601 gaaggcgccg acgctgacgc cctcctgctt ggatgcgagc aggagatcga gatcgaaacc
     9661 ctctgcggcc gcccgcgctc cccggctgtc ggagacgccc accagccggg cgccgcgtcg
     9721 ggcgagaaag cgggccgcgt gctggcccac ggagccgaat ccctggacca cgatcctggc
     9781 gccatcgagg ctgagatccg taaaggcctc ggcgacctcc agggaaacgc tcaagccaag
     9841 acccgtcgcg ccgatctcgt ccaagggaat gccccccagc tcccgcggca ggccggcgga
     9901 ccggccggtg acatccctga ccagtgccat ggaggtctcg tccgtaccca tgtcgggacc
     9961 gggtatgtag tctcgcaggt cggcgatggc gtgggcgaag cttcgaatca ggacttcctt
    10021 tgcttcgtcg gccatggccg ggtcggcgac gatcacagac ttggcgccgc cgtgagcgag
    10081 ccccgccatg gcgttcttca gcgtcattgc cctggcaagc cgcccacact cctcgcgcgt
    10141 ggcgtcgacg gccatcctga ccccgccgat ggccggcccg gcggcgacgt tgtccaccac
    10201 cacgatggcc ttcaggcccg tgctcgggtc gtaaatctcg atgaccttct cgggcccgag
    10261 ctcgtcgacg ttgtcgaaag tagcgatcat gaagcctcca gcgtttcccg agcggcgccg
    10321 acgattctcg acacgctcgg catgtaatga tgttcgagcc tggccagcgg catcacggtg
    10381 tcgtagccgg ccaccctcag gatgggtgcc tgcaggctgt acagcgcgcg ttcggccacg
    10441 catgcggcaa tttctccgcc gaaaccgccc gtgatcggcg cttcgtggac gatgacgaga
    10501 cggcccgttc gccccaccga ttcgcagatc gtctcgccgt ccaggggttt gatcgtggcg
    10561 acgtcgatga cctctgcggc gacgccttcg ccggacagct gctcggcggc ggccagggtt
    10621 tcctggacca tggctcccca ggtgacgagg gtgagatcct ttccgggacg gacgatgtgg
    10681 caacggtcga gcgggagggc tgcgccgtcg tcttcgaaag cctctttggc cgaccggtac
    10741 aggcgcttcg gctccaggaa gaccaccggg tccgggtccc ggatggctgc cagaagcagc
    10801 ccgtaggcgg tccgcggcga cgagggaatg accacccgca gccccggaat gtgagccagc
    10861 atggcctcgg cgctctcgga gtgatgttcc ggcgcgtgga tgccgccacc gaacggcgcg
    10921 cgcagcacca gcgggcagct ctggcgcccg cgggtgcggg tccgcattct cgaagcgtgg
    10981 ttcgcgagct gatcgatcgc ggcgtagatg aaccccatga actggatctc cgccacgggc
    11041 cggaacccct gcacggcgag gccgacggcc acgccggcga tgccggtctc ggccaggggg
    11101 gtatcgaaga cccggtgcgg cccgaaggcc tgcagcaggc cgtccgttgc cctgaacacg
    11161 ccgcccacca ccccaacgtc ctcgccgaga acgagaacac ggtcgtcatc ggccatggcg
    11221 cggcgcaacg ccaggttcac cgcctccagc agggtgtgct cagccatcgg ccggggcact
    11281 tgctagcagg gtcgctctct gtcgttccaa ctcccgggga aggctggcga agagatggtc
    11341 gaagatggcc tcgggtggct ggggcgtcgt cccgttgtaa gcggtggctg ccgacgccac
    11401 ctcgtcgtcg caccgctcga ccagggcatc ctcgtcggcg tcgctccatc cctcatgctg
    11461 ggcgagatag gcacccacgc gggccaaggg atccagctgc cgccagcgcg ccacctcggc
    11521 ctcgcttcgg tagcggctgg cgtcgtccac ggtggtgtga tcgcccagcc ggtaggtcag
    11581 gcactcgacc agtcgtggcc cgtcaccctt gcgggcttgg ccgatggcgt cgcccaccgc
    11641 ctcgctgacg gcaagcacat cgttgccgtc cacctggctg ccctcgaaac cggcggccac
    11701 cgctttctgc gccagggtgg tggccgccga ctgatcggct cggggcgtgg agatggccca
    11761 ctggttgttg ttcacgacga agacgacggg cagccgccag acgccggcga tgttcatcgc
    11821 ctcgtacaca tcgcccttgg acgtagcgcc gtcgccgaac acgcacacgg ccacccgggg
    11881 ttcggctcgg agcttcagcg ccagcgcgac gccggccgca tggggagcgt ggccgcccac
    11941 ggtaatgctg atgggcagat cgtccctcgg cacggcgtag tcggagccgc gctcgtcgcc
    12001 gccccagtag agcagcagct cgaccatgct cacaccccgg tacagctggg tgccctgatc
    12061 tctgaacgaa ggcaccagca cgtcctcggg cgccatggcg tgaccgatgc cgacgctggc
    12121 cgcctcctgg ccgagacagg aggcgtaggt tcccaggcgt ccggtccgct ggagctcgat
    12181 ggccttggcg tcgaaggcgc gggtgcggtg catccacccg tagagggctc ggatggtggc
    12241 cgggtccacc aggggctccg ggatcggccg caggagattg ccgtcgggcc cgatgatggt
    12301 gctggggtgg actccgaatg gaaggctgtc gctcatggtc ggctccggca cgtcgtgcgc
    12361 gtcaccagcg gaacttcagg gccagctccc acacgtcggc atcgaaagat tcaagctcgt
    12421 cctcattgta gtcgacgttg cggtagctga gcagcagggc gtagtccgcg ggcagcagaa
    12481 actcgacgcc cgcgcggacg tcgtcccggt cgaccctgaa gctgccctcg ttctcgtaag
    12541 ctcggtagct ggcggtcagg tccacccggt cggtgacatg ccaacgaacg gcgccgtccc
    12601 agaaatccgc atccgcgccg taggagatgg cgaacagatc ctgccgggag cccccggtca
    12661 ccagctggtc cacttcccgt tggagctcga cgaaggcggc tcccaccgac accgtcaggc
    12721 ggtcgcctgc gtgggtcagg cggatgtcgg actgacggct ttcggagatc cagcccgtat
    12781 tgtcgttttc gtagtccgtg cgccggtagg acgcggaaac cttcaggccg ttgtcccacc
    12841 ggtaggatgc gcgcgccttg tagcgtcggg catcggtagc cgatgccagc gtgtagggat
    12901 cgtcgatccg gtcgtccgcg aagctcaagg tgacctgggc tcttcggctt ggccggtagc
    12961 tgagccgccc gaagaagccg tcccggtcgg tatccacggc ttcggtgtgg ccggcgcctt
    13021 cgatggcgtg gctgacgacg ctcttccgcc gctcggtgga ccatccgccg ctgagtgtca
    13081 ccgtggtcgt cacggccatg tctaccccga gctcgaaccc ggtggtctcg atgtcccacg
    13141 cgctcgcgcc ggcggtgtcg ccgaacagca ccgcgccgtc ctgctccaga tcgtagcggc
    13201 gcacgccggc gttgagctgg agacgatcgt tgaccaggta ggcgaccgag gcgtcgagca
    13261 gcagggtgtc cctgtccacg tctgctgccc ccgcgtcctc gcggtcgaac ggacgcccga
    13321 ggtagtcggt gccctgggcc cgctccgttg ccgtcatgtc caggtccagg ttgccggccc
    13381 gcgcgtcgag ctccacgatc cactgggatg atggccgcac ctgcacgctc agacgctgat
    13441 cgctggacgt gaagccgtag ggctggtcca gaaaaaagag atccagcgag gtgggtgcgt
    13501 ccgcctcgct gcccggcgag aacccgggca gaaagatctg cgagtcgttg tcgtactcct
    13561 gccaggtctg gcccagggcc acggtgacgt gatcccaagc gtaagtgaag ccgagttcgt
    13621 atctgtccag ggactcgtcg atgggcttgt cgagctcgaa ttcctcccgc tccacgtcca
    13681 gcacggtggt gctctcgccg cgtttctcgt atcgggcgaa tcccaggttc agcttggccc
    13741 ggctgctcag atccagatcc agcgcgaggg cgtcgcgatt tctctcgaag tcgaaatgat
    13801 ggaagtcgcc gcctgtggac tgctcgacgc tggcttcctc gggtgcgagc agcaggtcct
    13861 cgtagaaata gtccgagctg cgatgatcat aggtgagccg gtagcttccg taacgttgaa
    13921 cgcgcacccg cacggattcg taggggtccc cgccgagccc gctggcgttg agctctaccc
    13981 ggtcgggcat gccggccggg ctattcgcgt cccggtcggg ctggaagacc atgccgagat
    14041 cgaacaggcg ggccccatca tcgaggttga cgtgctgttg gtacttgccc tcgtccccgg
    14101 acacgtcgac ctcgcggtag ccgacgctca cgtgaccggt gaaggtcgcg gcggcggcgc
    14161 tgcgggtact tgccgcaccg gcgatcagca gcgtgaaaac gatcaggagc cgagccgtca
    14221 tgagcccgtc acctcagaaa ggccgggtcc aggttggacc cgtgaatggt ggcgtggcag
    14281 ttcgtgcaca cggtgccttc gtcgaatcgg ggcgggcccg cggggctgaa gccgaggtgg
    14341 aactggggga cgatggcgtg acacgagtag cagagctcgc ccacctgctg gtgcgtgagc
    14401 atgtgccgat tcgggctgcc gtggggctcg tggcaggcgc cgcatccttc cacacgggac
    14461 gcgtcatgct cgaacacgaa cgggccgtcg acgtcggcgt ggcactcggc gcacaccctc
    14521 tggccgaagg cgccaagatg cccggagctc gccggcgcgt gggggtcgtg gcaggacgtg
    14581 caggaaacgc tgccttcctg taggcgatga cgctcgttga aggagaactg gctgaatgtc
    14641 tcctggtggc attcgaagca cgtggccgag gccgcgtcga cgcgatcgaa ctcggcaggc
    14701 agagcgattt cggctgcgct cgatgggtga atgctgtggc aatccgtgca agcgagcccg
    14761 gcctgggagt gcgggttggc gcgagcggtg tgagcgtccg cgtgacagct gccacaggcg
    14821 gcattgatcg tcgcgctggg ctcgtcatcg aatgcgagca gggatcccgg ctccgcgggt
    14881 gcggcgaggt gagttgccgg atcgccgtgg caactcctgc atccggacgc ttcgtcgtcc
    14941 tccgtccggg ccccgtgggg agtaccgtgg aagtccgcca cgagctcgcc gtggcaagcg
    15001 gcgcaggtcg ggtttccgcc gacctggccg gttgccagca gaaacagagt gcccaccgcc
    15061 attgaaaaca aacgcatggg tttagggtcc agcagcacgt gtcgctctgc tattgtgagt
    15121 tctacgtaga catactaccc gagggtcgtg tcagtttggt gccggcatgc tgatcgagat
    15181 caccacaggc cttgacgtcg aagtctccgg tcggccggag cagaccatcg atacgggtcc
    15241 ggaggtcggc caggttgccc tgcttggtgg ggactacctg aaccttcgcc cgtcgctcga
    15301 ggtggacgaa ggtgcccggg tcaaagcggg tgatacgatc ttcaccgacc gggcgcgtcc
    15361 ggaaattcg
//
LOCUS       DBNHOX010000273         1950 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_857809, whole genome shotgun sequence.
ACCESSION   DBNHOX010000273 DBNHOX010000000
VERSION     DBNHOX010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_857809"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..931
                     /locus_tag="AC16UZ_06025"
     CDS             101..931
                     /locus_tag="AC16UZ_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043711 - pilus organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis chaperone"
                     /protein_id="HIR6246277.1"
                     /translation="MTTCRWVWAVLLAAAAALLSPTALAGAVLFIHPTLVVFDEDTRS
                     ARITLTNRGDETGTFECGWMELTMNPEGALRRLEDQPGWSVAPHLRFSPRRVTLKPSQ
                     SQVIKIALRPDAKLPEGEYYSHFRILTLNSEAPRGPDIESRDEAPESSGVSVQARTAV
                     AIPIVWRNGDAVMAARIQSLSLNREEKQLTVEVEREGPLSSRGFVHVILAPPGEDAEP
                     FGDPAPLVIYPNLAGRSVTLDLPDLPDGNPTGELTVVYSTEGSSERKGRVLATRSMSI
                     "
     gene            948..1442
                     /locus_tag="AC16UZ_06030"
     CDS             948..1442
                     /locus_tag="AC16UZ_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246278.1"
                     /translation="MAPVRAAPALTACLLGCLLVQQTAIAGPAPRPIEILEVFSPSFG
                     TWLAGPSGRQLVLFTDDTVGGADAADYLFGARSGEFVISGSANQAINILVDNTFATGG
                     IVVNQVICQYHRDLPARCDGAGVDARSGNNRTLKIGIDITTSQAHTGGDAPTINMDVA
                     IIYL"
     gene            complement(1458..>1950)
                     /locus_tag="AC16UZ_06035"
     CDS             complement(1458..>1950)
                     /locus_tag="AC16UZ_06035"
                     /inference="COORDINATES: protein motif:HMM:NF028519.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HIR6246279.1"
                     /translation="QDEQPVPWASYRDTETIGSAPGTLRLRAMPRYQPVRIETRHLKF
                     DDPFLIPRESGYEIYTHAGSELSVDVAVVPTGDVEGYVFASAEDAGVSRAARDLAVSL
                     RSPDGRIVATARTEFDGFYCFTGVPPGHYQVHVGDASGRTVIESVVLDARVGLAQVDG
                     IHL"
BASE COUNT          354 a          661 c          591 g          344 t
ORIGIN      
        1 gcatccccaa cgaagtacgc gaccaccggg actcatggta tattgccttc atcagtcgta
       61 tttccctgtg ctaatgccga agatcacctg caaagccagc gtgacgacgt gccgatgggt
      121 ctgggccgtg ctgctcgccg ccgccgcagc gcttctttcc ccgacggcac tcgcaggcgc
      181 cgtgctgttc atccacccta ccctggtcgt ctttgacgaa gatacccgca gcgcccggat
      241 taccctgacc aaccgcggtg acgagaccgg cactttcgag tgcggctgga tggagctcac
      301 catgaatccg gaaggcgcgc ttcgcaggct ggaggaccaa cccggatggt cggttgctcc
      361 acacctgcgc ttctcgccgc gccgggtaac tctgaaaccg tcgcagtccc aggtcatcaa
      421 gattgccctg cgcccggatg cgaagcttcc cgagggcgag tactactcgc acttccggat
      481 tctgaccctc aacagcgaag ccccacgggg gccggacatc gaaagcaggg acgaggcacc
      541 cgaaagctca ggcgtcagcg tgcaggcccg aacggccgtc gcgattccga tcgtatggcg
      601 caacggggac gctgtcatgg ccgcgcgtat ccagtccttg agcctgaacc gggaagagaa
      661 acagctcacg gtggaagtcg aacgcgaggg tcctctttcc tcccgcggct ttgttcatgt
      721 gatattggcg ccgcccgggg aagacgccga gcccttcggc gacccggcgc ctctcgtgat
      781 ctacccgaac cttgcagggc gctcggtaac gctggacctc ccggatctgc cggacggaaa
      841 cccaacgggg gagctgacgg tggtctactc caccgagggc agttccgagc gcaagggccg
      901 ggtcctggct acccgttcca tgagcatctg agagcgcttg ggctggagtg gcacccgtcc
      961 gcgccgcgcc cgcactgacc gcgtgcctgc tgggctgcct gctcgtgcag cagacggcca
     1021 tcgccgggcc ggcgccgcgc ccgatcgaaa tactcgaggt tttctcgcca agcttcggga
     1081 cctggcttgc aggccccagc ggtcgccagc tcgtgctgtt cacggacgat accgttggcg
     1141 gcgctgacgc ggccgactat ctcttcggcg caagatccgg agagttcgtg atttccggct
     1201 ctgctaacca ggcgatcaac atcctcgtcg acaatacgtt cgctaccggc ggtatcgtcg
     1261 tcaatcaggt catctgccag taccatcgcg acttgccggc acgctgcgat ggggccggcg
     1321 tcgacgcccg atccggaaac aaccgaacgc tgaagatcgg catagacatc acgacgtccc
     1381 aggcacacac cggcggagac gcgccgacca tcaacatgga cgtcgccatc atctatctct
     1441 gatgggcggg cgcaccctca caggtggatg ccgtctacct gtgcaaggcc aactctggca
     1501 tcgagcacca cggactcgat caccgttctg cctgaggcat ccccgacgtg gacttgatag
     1561 tgcccgggtg ggacacccgt gaaacagtag aagccatcga actcggttcg cgcggtcgcg
     1621 acgattctgc catcaggcga tcgcagcgag acggcgaggt ctctggccgc cctggagaca
     1681 cccgcgtcct cagccgatgc gaacacgtag ccctccacat ctccggtcgg caccacggcc
     1741 acatccacgc tcagctcact ccctgcgtgg gtgtagattt catagccgct ttcccgcggg
     1801 atcaggaacg ggtcgtcgaa cttgaggtgg cgcgtctcga tccgaaccgg ctgatacctt
     1861 ggcattgccc gcaggcgaag ggtgcctggc gccgagccga tggtctccgt gtcgcgataa
     1921 gacgcccacg gcaccggctg ctcgtcctga
//
LOCUS       DBNHOX010000274         3322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1501388, whole genome shotgun sequence.
ACCESSION   DBNHOX010000274 DBNHOX010000000
VERSION     DBNHOX010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1501388"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="AC16UZ_06040"
     CDS             <1..615
                     /locus_tag="AC16UZ_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246280.1"
                     /translation="MEQEAAVADMGDYRLEIPAFMKEIVAEITHHARRSPDINQRSGV
                     SVRTSIADYEAMLANAFRRALRLGEDEVVPRISDLAFVLPSFQGKVEFEAMEEGQEDK
                     ITQRIMQAAIKSVFDRYYDVNDLESIALAFNDGLTVDTGEATPAADYNAIGERIPGLM
                     DVFPDREGVSPGVRAAIIEFILEGLHLHKLLNKFDNRGAATYSG"
     gene            646..2739
                     /locus_tag="AC16UZ_06045"
     CDS             646..2739
                     /locus_tag="AC16UZ_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6246281.1"
                     /translation="MRHFPSGYRFSRWDGSQKLPLDADEIMAALADDLMEYGDLRWAM
                     SNLMSRGMQIPQGGYMQGLRDMLRQLRERKREQLERFDLSSVFEDFRERLDEILAMER
                     KRIDEWLDPKPATDSEAPAGNGPSGEPEQSTSADQPAAQGDGPDFSGDVLKQIAQRNR
                     DELDGLPPDLAGQVRTLEKYEFLNPDAQRKFLELLNELRRAMTNTFFNDIENMVNQLS
                     DGDVERMKEMIKALNEMLVKRIAGEDPGFEDFMAQYGDMFGDDPPQSLDDLLERMAQQ
                     MAAAQSLLNSMSPEQQQQLQSLLSDRFGDPELNSELRKLAKEMDFLNPDGGKYRFSGD
                     ERLDLETAMKLMSEMQDLDELISQVQEAERGGGTDRIDRDLLSDLLGDEAADDLEQLQ
                     QMLKALEEAGYIRPTDDDGWEITPRGSRMIGQKALGEIYARLKRQNLGNHAVPEEGRF
                     GERLEQTKAYEFGDPFHLHMPRTLRNAIDREGPQTPVSLKPEDFEIYRSELITSTATA
                     MLVDLSWSMALRGSFQAAKKVALALHNLITSQYPKDTFYIIGFAAYAKELKPKDLPYL
                     QWDEYVLGTNMQHALLLAERLLAKHQAGSKQIIMISDGEPTAHLENGQAQFAYPPTPE
                     TIRATFRAVKHCTARGVAINTFMLDSSYYLKAFMDEIAKINGGRVFYTTPEQLGEYIL
                     VDYVQNKKKRLARRA"
     gene            complement(2740..>3322)
                     /locus_tag="AC16UZ_06050"
     CDS             complement(2740..>3322)
                     /locus_tag="AC16UZ_06050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6246282.1"
                     /translation="PEVTISAIDKAGRNAVASCEVVYDDLPVAVTDRVGEEGKGFRYL
                     LDGLNAERILVASEALGIGRAAMRRAVDYANDRVVFNRPIGQNQGVAFPLGEAQMRLD
                     AAELMIRKASWLLDKGSPCGAEANMAKWLAADAAYQAADQAMQTHGGFGYAKEYHVER
                     YWREARLMRIAPISQEMILNYVTEHVLGLPRSY"
BASE COUNT          663 a         1092 c         1038 g          529 t
ORIGIN      
        1 atggagcagg aagcagccgt ggccgacatg ggcgattacc ggctcgaaat ccccgccttc
       61 atgaaggaga tcgtcgcgga gatcacccat cacgcccggc gctcgcccga catcaaccag
      121 cgatcgggcg tttccgtgcg aacgtccatc gccgattacg aagcgatgct ggcgaacgcc
      181 ttccggcggg cgctgcgcct cggcgaggac gaggtggtgc cgcgcatctc cgatctggcg
      241 ttcgtgctcc cgtcgtttca gggcaaggtc gagttcgagg ccatggagga aggccaggag
      301 gacaagatca cccagcggat catgcaggcc gccatcaagt cggttttcga tcgctactac
      361 gacgtgaacg atctggagtc cattgccctg gctttcaacg acgggctcac ggtggatacc
      421 ggcgaggcca cacccgcagc ggactacaac gccatcggcg agcgcatacc cggcctgatg
      481 gacgtgtttc ccgacaggga gggcgtaagc cccggcgtgc gggcggccat catcgagttc
      541 atcctggagg ggctgcacct gcacaagctt ctcaacaagt tcgacaacag gggagctgcc
      601 acctactcgg ggtgagcggc aacccacacc acagctgagg ccgtcatgcg tcactttccc
      661 tcaggctacc gattctcccg ctgggacggc tcccagaagc tgccgctgga cgccgacgag
      721 atcatggccg cgctggccga cgacctgatg gaatacggcg accttcgctg ggccatgagc
      781 aacctcatgt cacgcggcat gcagatacct cagggcggct acatgcaggg cttgcgggac
      841 atgctccggc agctgaggga acgcaagcgg gagcagctcg agcgcttcga cctgtcgagc
      901 gtgttcgagg acttccggga gcggctcgac gagatcctcg ccatggagcg aaagcgcatc
      961 gacgagtggc tcgatccgaa gcccgcaacg gattccgaag ctcccgctgg caacgggccg
     1021 agcggcgaac ccgagcagag cacgtctgcc gaccagcctg ccgcccaggg ggacggtccc
     1081 gatttctccg gcgacgtgct caagcagatc gcccagcgca accgggacga gctcgacggg
     1141 ctgccgccgg atctcgccgg gcaggtccga accctggaga agtacgagtt tctgaaccct
     1201 gacgcccagc gaaagttcct ggagctgctc aacgagctgc gccgggccat gacgaacacg
     1261 ttcttcaacg acatcgagaa catggtcaac cagctctccg acggcgacgt cgagcgcatg
     1321 aaggagatga tcaaagcatt gaacgagatg ctggtgaagc ggatcgccgg ggaggatccg
     1381 ggattcgagg atttcatggc ccagtacggg gacatgttcg gtgacgatcc gccccagtcc
     1441 cttgacgatc ttctggagcg catggcccag cagatggcgg ccgctcaatc cctgctgaac
     1501 tccatgtcgc ccgagcagca gcaacagctg cagtccctgc tcagtgatcg cttcggcgat
     1561 ccggagctga actctgagct tcgcaagctc gccaaggaga tggacttcct caacccggac
     1621 ggcggcaagt accggttctc cggagacgag cggctcgatc tcgaaacggc catgaagctg
     1681 atgagcgaga tgcaggacct cgatgagctg atctcccagg tccaggaagc agagcgcggt
     1741 gggggcacgg accgtatcga tcgggacctg ttgagcgacc tgctcgggga cgaagcggcc
     1801 gacgacctcg agcagctgca gcagatgctc aaggccctgg aggaagcggg ctacatccgt
     1861 cccaccgacg acgacggctg ggaaatcacg ccaaggggtt cccgcatgat cggccagaag
     1921 gcccttggcg agatctacgc caggttgaag cggcagaacc tcggcaatca tgccgtgccc
     1981 gaggaggggc gtttcggcga gcggctggag cagaccaaag cctacgagtt cggcgacccg
     2041 ttccatctgc acatgccccg aaccctgcgc aacgccatcg atcgggaggg cccgcagacg
     2101 ccggtgagcc tcaagcccga ggacttcgag atctaccgca gcgagctcat cacgtccacg
     2161 gccacggcca tgctggtgga cctgtcctgg tccatggcgc tcaggggctc tttccaggcg
     2221 gcaaaaaagg tggcgctggc gctgcacaac ctcatcacct cgcaatatcc caaggacacc
     2281 ttctacatca tcggctttgc cgcttacgcc aaggagctga agccgaagga tctaccctac
     2341 cttcagtggg acgagtacgt gctggggacc aacatgcagc acgccctgct gctcgcggag
     2401 cgtctcctgg ccaagcacca ggcaggcagc aagcagatca tcatgatctc tgacggggaa
     2461 cccaccgccc atctggagaa cggccaggcc cagttcgcct acccgcccac acccgagacc
     2521 attcgcgcca cctttcgcgc cgtgaagcac tgcaccgcgc ggggcgtggc gatcaacacg
     2581 ttcatgctgg attcgagcta ctacctcaaa gccttcatgg acgagatcgc caagatcaac
     2641 ggcggccgcg ttttctacac gacgcccgag cagctcggcg agtacatcct cgtcgactac
     2701 gtgcagaaca agaagaagcg gctcgcgcgc agggcctgat cagtaagagc gcggcagccc
     2761 gagcacgtgc tcggtgacgt agttcaggat catctcctgg gagatcggcg cgatccgcat
     2821 gagcctcgct tcccgccagt agcgctccac gtggtactcc ttcgcgtaac cgaacccgcc
     2881 gtgggtctgc atggcctgat ccgccgcctg gtaagccgcg tcggcggcca gccacttggc
     2941 catgttggcc tccgccccgc agggcgaacc tttgtccagc agccaggacg ccttgcggat
     3001 cataagctcc gccgcgtcca ggcgcatctg cgcttcgccc agaggaaagg ccacgccctg
     3061 gttctggccg atcggccgat tgaagaccac gcgatcgttg gcgtagtcca ccgcccgacg
     3121 catggccgcg cggccgatgc cgagcgcttc cgaggccacc agaatacgct cggcgttgag
     3181 gccatcgagc aggtaacgga aacccttgcc ctcctcgccg acgcggtcgg tgaccgccac
     3241 aggcaggtcg tcgtagacca cctcgcagct ggccaccgcg ttgcgccccg ccttgtcgat
     3301 ggctgagatg gtgacctcgg gt
//
LOCUS       DBNHOX010000275         3036 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1243459, whole genome shotgun sequence.
ACCESSION   DBNHOX010000275 DBNHOX010000000
VERSION     DBNHOX010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1243459"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="AC16UZ_06055"
     CDS             complement(<1..362)
                     /locus_tag="AC16UZ_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine nucleotide alpha hydrolase family
                     protein"
                     /protein_id="HIR6246283.1"
                     /translation="MAETRKVFVVLDPTTMEQASLQWGEKIALDNRAHRSQDTHLHVY
                     CCVNFKSVTAVAADTFEHVEAATLERVREWVNRIVTHTRSLGVPVDVEVEWDADWRKA
                     IVGAVRRQKPDVVIKNLT"
     gene            460..1674
                     /locus_tag="AC16UZ_06060"
     CDS             460..1674
                     /locus_tag="AC16UZ_06060"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase family protein"
                     /protein_id="HIR6246284.1"
                     /translation="MERICEISPLAHLPDDRMPNADPFAPEIPEPVLEDLRRRLTTIR
                     WPHVIGEDDWVYGVPQAWLKDMADYWAHEWNWNSTATAMAAFDHYLADMDGIPVHFLH
                     VPGKGPAPKPLILTHGWPWTFWDYKDVIGPLSDPAAFGGDAADAFDVVVPSLPGFGFS
                     TPLTSAGIDVAEVARLWVRLMQDVCGYERFAAHGGDWGALVTAHLGHAHAQSLIGAHL
                     ALPLIPGVDRASVPREAYAEDEQWMLERAAQSGPLIRSHVSVHTTDPQTLAYAMSDSP
                     IGTAAWIWERRRNWSDCDGDVERAFDRDHLCTTAALYWCTGAFTSSLRLYFEHFNKPW
                     PLVHDRKPTVEAPTGFAIFPKDVVQMPRRVAAEHTDLRRWSVMPRGGHFGAAEAPDLI
                     VDEIRSFFRDLS"
     gene            1691..>3036
                     /locus_tag="AC16UZ_06065"
     CDS             1691..>3036
                     /locus_tag="AC16UZ_06065"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6246285.1"
                     /translation="MAKHHAHDLSRPSDPNLAPGAARCPGPSFQDIVRTEDSARSTAL
                     TTQSYDFLGDEDVPFSRYTSREMYELEIERVWSRTWQWACREEHIPSPGDYHVYDLGP
                     YSVLVIRSDPNTVRAFVNSCPHRGMQFCDSGTSGRGKQFLRCPFHGMSWHLDGSLREI
                     PCRWDFPHVEEENFGLTPVACETWGGFVFISLDPEPEPLATYLGVLPEHFSEWPLEER
                     YVALHMQKVLPGNWKMCMEGFMEAYHVMATHPEGLKTSAWATTQYDVFGRHVSRFMQP
                     RAMPSPYYGRPVSEREAFAALGHDPEDFPEGSSARQAHADSVRRSMGESLGVDLTGYT
                     NSQLLDSIEYHLFPNACLFPGIIVPFIYRFRPLTWDSCLHDILVLHPVPDSGPRPPPA
                     EPIRLGIDDSYTSVPGFSENRLALVLDQDTDNFRRQWAGMQASRKPGETLANYQEVR"
BASE COUNT          527 a         1018 c          925 g          566 t
ORIGIN      
        1 tgggtgaggt tcttgatcac cacgtcgggc ttctgccgcc ggacggcccc cacgatggcc
       61 tttcgccaat ccgcgtccca ctccacttcc acatcgacgg gcacgccaag cgaccgggtg
      121 tgcgtgacga tcctgttcac ccattcgcga acccgctcaa gggtggccgc ttcgacgtgc
      181 tcgaaggtgt cggctgcaac cgcagtcacc gacttgaagt tgacgcaaca gtaaacgtga
      241 agatgggtgt cctgggaccg gtgagcccgg ttgtccagag ctatcttctc gccccattga
      301 agcgacgcct gctccatcgt cgtgggatcg agcaccacga acacttttct cgtctctgcc
      361 atcgactgct gactccgcgt tgatctcgat tggtcaagtt tcaccgcatc ggcgcggcgg
      421 gcatatcggc acttccccgt agacgccacg gttgtctaga tggagcggat ctgcgagatt
      481 tccccactgg cccatcttcc ggacgatcga atgcccaatg ccgatccgtt tgcgccggag
      541 attcccgagc ccgtgctcga ggacctgcga cggcgcctca cgactatccg atggccccat
      601 gtcatcggcg aggacgactg ggtttacgga gttccccagg cctggctgaa ggacatggcc
      661 gactactggg cccacgaatg gaactggaac tcgaccgcta ccgccatggc ggccttcgac
      721 cactacctgg ccgacatgga cggcataccc gttcactttc ttcacgtccc gggcaaaggc
      781 ccggcgccaa agccgctgat cctgactcac ggatggccct ggaccttctg ggactacaag
      841 gatgtcatcg gcccgctgag cgacccggcc gcgtttggcg gcgatgcagc tgacgccttc
      901 gacgtggtgg tgccctcttt gcccggtttc ggattttcca ccccgctcac cagtgccggt
      961 atcgacgtcg ccgaggtagc ccggctctgg gttcggctca tgcaggatgt gtgcgggtac
     1021 gagcgcttcg ccgcccacgg cggtgactgg ggcgccctgg tgacggctca tctgggccat
     1081 gcgcacgcgc agagcctgat cggcgcgcac ctggcgctgc cgctgattcc cggcgtcgac
     1141 cgagcctccg tacccaggga ggcctacgcg gaggacgagc aatggatgct cgaacgcgca
     1201 gcgcaatccg gccctctcat tcgcagccac gtgagcgtgc acacgacgga tccgcaaacc
     1261 ctggcgtacg cgatgtccga ctcgcccatc ggaacggcgg cgtggatctg ggagcggcgc
     1321 agaaactgga gcgactgcga cggcgacgtg gagcgcgcct tcgaccgcga tcacctgtgc
     1381 accaccgcag cgctgtattg gtgcacgggc gctttcacca gctcgctgcg actgtatttc
     1441 gagcacttca acaagccctg gccgctggtg cacgacagga agcccaccgt agaagcgccc
     1501 acgggcttcg ccatatttcc caaggacgtg gtgcagatgc cacggcgggt tgccgccgag
     1561 cacacggatc tccgccgctg gagcgtcatg ccgcgaggcg gacacttcgg agcggcagaa
     1621 gctcccgatc tgatcgtcga tgagattcgc agctttttcc gcgacttgag ctgacaggag
     1681 cgccgagatc atggccaagc accacgcaca cgacctgagc cgcccctccg acccgaacct
     1741 ggcgcccggc gcagcgcgct gccccgggcc gagctttcag gacatcgtca ggaccgagga
     1801 cagcgctcgg tccacggctt tgaccacgca gtcctatgat tttctgggcg acgaggacgt
     1861 gcccttctct cgctatacgt ccagggaaat gtacgagctg gagatcgagc gtgtctggag
     1921 ccgcacatgg cagtgggcct gcagagagga gcacattccc tcgcccggtg attaccacgt
     1981 ctacgacttg gggccttact cggttctcgt gatccgcagc gatcccaaca ccgtccgcgc
     2041 gtttgtcaat tcctgtcccc accgcggcat gcagttctgc gactcgggta cgtccggacg
     2101 gggcaagcag tttctgcgct gcccgttcca cggcatgtcc tggcacctgg acggttcgct
     2161 gcgagagatt ccctgccgct gggacttccc ccacgtcgaa gaggaaaatt tcggtctgac
     2221 gcctgtcgcg tgcgagacct ggggcggttt cgtgttcatc agcctggatc ctgagccgga
     2281 gccgttagcg acctatctcg gcgtgctgcc ggagcacttc agcgagtggc cgctcgagga
     2341 gcgctacgtc gcgctgcaca tgcagaaggt tctgcccggc aactggaaga tgtgcatgga
     2401 aggattcatg gaggcctatc atgtcatggc tacgcatccg gagggcttga agacatctgc
     2461 ctgggctacg acgcagtacg atgtcttcgg gcgccacgtg agccgcttca tgcagccccg
     2521 ggcgatgccg agcccctact acgggcggcc cgtcagcgag agagaggcgt ttgccgcgct
     2581 cggacacgac ccggaagatt tcccggaggg ttcaagcgcc cggcaggcgc acgcggactc
     2641 ggttcgtcgc agcatgggcg agtctctcgg cgtcgacctg acgggataca ccaacagcca
     2701 gctgctcgac tccatcgagt atcacctgtt ccccaacgcc tgcctcttcc ccggcatcat
     2761 cgtcccgttc atctaccgct ttcgtccgct cacgtgggac agctgtctgc acgacatcct
     2821 cgttctgcat ccggtgcccg atagcggtcc gcgtccccca cccgccgagc ccattcgcct
     2881 gggaatcgac gactcgtaca catcggttcc cggattcagc gagaatcgac tggcactcgt
     2941 gctggatcag gacaccgaca acttccgccg ccagtgggcg ggcatgcagg cctccagaaa
     3001 gccgggggaa acccttgcca actaccagga ggtgcg
//
LOCUS       DBNHOX010000276         3527 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_87482, whole genome shotgun sequence.
ACCESSION   DBNHOX010000276 DBNHOX010000000
VERSION     DBNHOX010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_87482"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /gene="htpG"
                     /locus_tag="AC16UZ_06070"
     CDS             complement(<1..1170)
                     /gene="htpG"
                     /locus_tag="AC16UZ_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HIR6246286.1"
                     /translation="MSEQPAQETFGFQTEAKQLLKLMIHSLYSNREIFLRELISNASD
                     AIDKLRFRSISDPSLAGDYAEFAITVSFDEDAGTVTVADNGIGMTRDEVIEHLGTIAK
                     SGTAEFFAQLSGDQKKDAALIGQFGVGFYSAFIVADEVEVLTRGAGTEQGTRWVSKGE
                     EDFSVADAPDVPRGTRVTLHLKEDAKEFADSFRLQSVVRRYSDHIGVPVRMPKVGDGE
                     DAGPEYETVNSAKALWTRSRSEVEDDEYQEFYKHVSHDFDDALGWSHNRVEGKLEYTS
                     LLYIPKHAPFDLWNREAPRGLKLYVQRVFIMDEAEQFLPLYLRFVKGVVDSNDLPLNV
                     SREILQQDDRVTTIRNALTKRVLNVLDAMAANEPEKYQSFWDEFGEVLKEGAGEDF"
     gene            complement(1228..2103)
                     /gene="sucD"
                     /locus_tag="AC16UZ_06075"
     CDS             complement(1228..2103)
                     /gene="sucD"
                     /locus_tag="AC16UZ_06075"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR6246287.1"
                     /translation="MSILVDSSTRVICQGFTGAQGTLHSEQAIAYGTQVVGGVTPGKG
                     GSEHLGRPVFNTVADAVEATGATASVIYVPAPFCKDSILEALDAGIELVVCITEGIPT
                     LDMLAVKAKLDTTSSRVIGPNCPGVITPGECKIGIMPGEIHLPGRVGIVSRSGTLTYE
                     AVKQTTDRGFGQSSCVGIGGDPIPGSHFIDILALFEADPATEAIVMVGEIGGTAEEEA
                     AEFIRSNVSKPVVSYIAGVTAPPGKRMGHAGAIIAGGKGTAAEKFAALEAAGVRTVRS
                     LAEIGDAIAEVTGWS"
     gene            complement(2100..3269)
                     /gene="sucC"
                     /locus_tag="AC16UZ_06080"
     CDS             complement(2100..3269)
                     /gene="sucC"
                     /locus_tag="AC16UZ_06080"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR6246288.1"
                     /translation="MNLHEYQAKQLFREYGLPVSEGYAAGTAQEAVEAASKIGGEKWV
                     VKAQVHAGGRGKAGGVKLVSTKQEIEDFAHQWLGKNLVTYQTDANGQPVNKILVESCT
                     DIDRELYLGAVVDRGTRRVVFMASTEGGVEIEKVAEETPEKILRVAIDPFVGPQPYQG
                     RQLAFRLGLEGAQIREFTNLFMGLSRLFQELDCSLLEINPLVITTAGNIHCLDAKVNI
                     DGNAMFRQPALAAMRDVSQEDEREAEAAEYDLNYVALDGNIGCMVNGAGLAMGTMDLV
                     KIHGGNPANFLDVGGGATKEAVSEAFKIILSDENVKAVLINIFGGIVSCAIIAEGIVG
                     AVQEVGVEVPVVVRFEGNNAPRGREVLAASGLNIIAAESLADAARKAVAAAGGRP"
BASE COUNT          621 a         1177 c         1033 g          696 t
ORIGIN      
        1 gaaatcctcg ccggcgccct ccttgagcac ctcgccaaac tcgtcccaga acgattggta
       61 tttctcgggc tcgttggccg ccattgcgtc cagcacgttc agcacgcgct tggtcagggc
      121 attgcgaatc gtcgtgacgc gatcgtcctg ctgaagaatt tcccgcgaca cgttcagggg
      181 taggtcgttg gagtccacca cgcccttgac gaagcgcagg tagagcggaa ggaactgctc
      241 ggcctcgtcc atgatgaaca cccgctgcac gtagagcttg agcccccgcg gcgcttcccg
      301 attccaaaga tcgaacgggg cgtgcttggg tatgtagagc aggctggtgt actcgagctt
      361 gccctccacc cggttgtgac tccagccaag ggcatcatcg aagtcgtgag aaacgtgctt
      421 gtagaactcc tggtactcgt cgtcctcgac ctcgctgcgc gaccgggtcc acagcgcctt
      481 ggccgagttg acggtttcgt actccgggcc ggcgtcctct ccatcaccga ccttgggcat
      541 ccggacggga accccgatat gatccgagta ccggcggacg acgctctgca gccggaaaga
      601 atcggcgaac tccttggcat cttccttgag gtgaagggtc acgcgggtcc ctctcggcac
      661 gtcaggcgca tccgcgacgg agaagtcctc ctcgcctttg gagacccagc gggttccctg
      721 ctcggtgccg gcgcctcggg tcagcacctc gacctcgtcg gcaacgatga aagcgctgta
      781 gaaaccaacg ccgaactgcc cgatcagggc cgcatccttc ttctgatctc cgctgagctg
      841 agcaaagaac tccgccgtac cggacttggc aatggtgccg aggtgctcga tcacctcgtc
      901 gcgcgtcatc ccgatgccgt tgtcggcaac cgtcacggtg cccgcgtcct catcaaaaga
      961 cacggtaatg gcaaactcgg cgtagtcacc tgccaggctg gggtccgaaa tggaccggaa
     1021 gcgaagcttg tcgatggcgt ccgaggcgtt ggatatgagc tcgcgcagaa agatttccct
     1081 gttcgagtac agagagtgaa tcatcagctt gagcagttgc ttggcttccg tctggaagcc
     1141 gaaggtctcc tgggctggtt gctcagacat ggctacccgt cacgtgatcc ccgcgttggc
     1201 atctatatgg ctgctaaagc cggaatttca agaccagccg gtcacctcgg caatggcgtc
     1261 gccaatttct gccaggcttc gcaccgtgcg cacgcccgca gcctcaagag ccgcaaattt
     1321 ctcggccgcc gtgcccttgc ctccggcgat gatcgccccg gcatgaccca tacgcttgcc
     1381 tggcggggcg gtaaccccgg cgatgtagga aaccacgggt ttggagacat tcgatcgaat
     1441 gaactcggcc gcttcctcct ccgccgttcc accgatctcc cccaccatca cgatggcttc
     1501 ggtcgccggg tctgcttcga acagcgccag gatgtcgatg aagtggctcc caggaatggg
     1561 atcaccgccg atgccgacgc agctgctctg gccgaatccg cggtccgtag tctgctttac
     1621 cgcttcgtaa gtgagggttc ccgagcggga cacgatgcct acgcggcccg gcagatggat
     1681 ctcgcccggc atgatgccga tcttgcactc gcccggcgtg atgactccag ggcagttggg
     1741 cccgatgacg cggctgctcg tcgtgtcgag cttggctttc accgcgagca tgtccagggt
     1801 cggtatgcct tcggtgatgc agaccacgag ctcgatgccg gcgtccagcg cctcgaggat
     1861 ggagtccttg cagaacggag cgggcacgta gatcaccgac gcggtggcgc cggtggcttc
     1921 cacggcatcc gccaccgtat tgaacaccgg tcgtccaagg tgctcggaac cacctttgcc
     1981 gggcgtgacg ccgccgacta cctgagtgcc gtaggcaatg gcctgctcgc tgtgcagggt
     2041 gccctgggcg cccgtgaagc cctgacagat gacccttgtg ctgctgtcga ccagaatgct
     2101 catggccgtc ctcctgccgc tgccacggct ttccgggcgg cgtcggccag actctccgca
     2161 gcgatgatgt tcaaaccgct ggcggcgagc acctcgcgtc cccgcggggc gttgttgccc
     2221 tcgaaacgca ccaccacggg cacctcgacc cccacttcct ggacggctcc cacgatccct
     2281 tcggcgatga tggcgcacga gacgatgccg ccgaatatgt tgatcaggac ggccttcaca
     2341 ttctcgtccg agagaatgat cttgaaggcc tccgaaaccg cctccttggt cgcgccgccg
     2401 ccaacgtcga ggaagttggc cgggttgccg ccatgaatct tcaccagatc catggtgccc
     2461 atggccaggc ccgcgccgtt caccatgcaa ccgatgttgc cgtcgagcgc gacgtagttc
     2521 agatcgtact cggcagcttc ggcttcccgt tcatcctcct gggagacgtc gcgcatggcc
     2581 gcaagggctg gctggcgaaa catcgcgttg ccgtcgatgt tgaccttcgc atcaaggcaa
     2641 tgaatattcc ccgccgtggt aatcaccagc gggttgatct ccagaagcga gcaatccagt
     2701 tcctgaaata gcctagataa acccatgaaa agatttgtaa attcccttat ctgagcgccc
     2761 tctagaccaa gccggaaggc cagctgacgg ccctgatagg gttgtggccc tacaaaggga
     2821 tcgatggcaa cgcgaaggat cttctccggc gtctcctctg ccaccttctc gatctcgacg
     2881 ccaccctcgg tggaggccat gaacaccacc cggcgcgtgc cgcgatcgac cacggcaccg
     2941 aggtacagct cgcggtcgat gtcggtgcac gactcgacca ggatcttgtt gaccggctga
     3001 ccgttggcgt cggtctggta ggtgaccagg ttcttgccca gccactggtg ggcgaaatcc
     3061 tcgatctcct gcttcgtgct gacgagcttg acgccgcccg ccttgccgcg accgccggcg
     3121 tggacctggg ccttgaccac ccacttctcg ccgccgatct tgctcgccgc ctcgaccgcc
     3181 tcctgtgcgg tgcccgccgc gtacccctcc gagaccggca gcccgtattc cctgaacagc
     3241 tgcttcgcct ggtattcatg cagattcatg tcgtttcccc acgtaacgaa aacatgtgat
     3301 tatcggcaca taaaatatca ttatacctgc gtaccctcgc cccagtacac ccgtatcacg
     3361 gcgcagaccc ggagcgactg gcccggcggc gaggccgggg cctacaatcg cccatcatgt
     3421 ggcggttcct gctcgtgttg ctggcgatct ggatcgccct ggtgctctac cgcgacgccc
     3481 gccggcgccg tgcccggcgt cgtaagcccc tgccccccgt gaccacg
//
LOCUS       DBNHOX010000277         2322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_87636, whole genome shotgun sequence.
ACCESSION   DBNHOX010000277 DBNHOX010000000
VERSION     DBNHOX010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_87636"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="AC16UZ_06085"
     CDS             <1..494
                     /locus_tag="AC16UZ_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246289.1"
                     /translation="ERVAPWQAGSRTLFQSQPGQAPADAAGLVDQLCARLGNRACHGI
                     RPVDQHAPEQAWRRSRPAMNGRGGRRSRSRSGKHGNGRATDGEEALLPAPDERPIWLL
                     SRPRPINRSELKLLKGPERIRTAWWQRGISRDYYVARDRRGAECWAFVDAGNNWYLHG
                     YFA"
     gene            478..>2322
                     /locus_tag="AC16UZ_06090"
     CDS             478..>2322
                     /locus_tag="AC16UZ_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR6246290.1"
                     /translation="MATSPDKPYDGGFAELHCISNYSFLRGASHPEELVKRAAELGYR
                     ALALTDECTYAGLVKAHVAARELGIKLIVGAEFRLEEGIHLILLAPDRSAYGQLSALI
                     TKLRRRSPKGAYEVCLDDFRWGVDECLALWVAGDGDIETLMAQGRRLSERWRELWIAL
                     ELFREDKDLDKMANALTLSMRLDLPIVASNDVHAHLPARQPLQDVLTAIRLKTTVAEL
                     GCAAHRNGERHLRSLSELVSLYPPEMLEASCQIADRCRFSMDELRYEYPEELVPPHLT
                     PGEYLRQLTLAGAAERWPDGVPADTLALIERELTLIREMRYEYYFLTVQDIVQFARSQ
                     QILCQGRGSAANSAVCYCLHITEVDPSRMHLLFERFVSKERNEPPDIDVDFEHERREE
                     VIQYIYKRYGRDRAALAATLITYRPKSAVRDVGKALGMDLDLVDLLAKSLAWWDRREA
                     LNERFAAAGLDPDSPVVGHFLRFVAEILGFPRHLSQHVGGFVISQGPLAQLVPVENAA
                     MADRTIIQWDKDDLEALGLLKVDVLGLGMLTAIRKALASLNAYRGTDLAVARIPPEDP
                     ETYRMLQRGDSVGVFQVESRAQMAMLPRLKPQHFYDLVIEVAIVRPGPI"
BASE COUNT          382 a          825 c          746 g          369 t
ORIGIN      
        1 ccgaacgcgt ggcgccctgg caggccggca gccggacact ttttcaatcc cagcccgggc
       61 aggcccccgc agacgctgcc gggctcgtcg atcagctttg cgcccggctg ggcaaccggg
      121 cctgccacgg catccggccg gtggatcagc acgcgcccga gcaggcctgg cgtcgttcac
      181 ggcctgccat gaacgggcgc gggggcaggc gcagtcgcag tcgcagcggc aagcatggca
      241 acggcagggc gacggacggg gaagaagcgc tcctgcccgc gcccgacgag cggccgatct
      301 ggctgctcag ccggccgcgc ccgatcaaca ggagcgagct gaaactgctc aagggccccg
      361 agcgtatccg cactgcctgg tggcagcgcg gcatcagccg ggattactac gtcgcccggg
      421 accgccgggg cgctgaatgc tgggccttcg tcgacgcggg gaacaactgg tacctgcatg
      481 gctacttcgc ctgacaagcc gtacgacggt ggcttcgcgg agctgcactg cataagcaac
      541 tacagctttc tgcgcggagc ctcccatccg gaggagctcg tcaaacgggc cgccgagctc
      601 ggctaccgcg cgcttgcgct gaccgacgag tgcacctatg cgggactcgt caaggctcac
      661 gtagcggcgc gcgagctcgg catcaagctc atcgtgggcg cggagttccg cctcgaggaa
      721 ggcattcacc tcatcctgct ggcgccggac cgcagcgcct acgggcagct ctccgcgctg
      781 atcaccaagc tgcgcagacg ctcccccaag ggcgcctacg aggtctgcct ggacgacttc
      841 cgctggggcg tggatgaatg ccttgcgctg tgggtagccg gcgacggcga catcgaaacc
      901 ctcatggccc agggcagacg gctcagcgaa cgctggcgcg agctctggat tgctctcgaa
      961 ctcttccggg aggataagga cctcgacaag atggccaacg ccctgaccct gtccatgcgc
     1021 ctggatctgc ccatcgtggc cagcaacgac gtccacgcgc acctccccgc ccggcagccc
     1081 ctgcaggacg tcctcaccgc cattcgcctg aagaccacgg tcgccgagct cggctgcgcg
     1141 gcccatcgca acggcgagcg ccacctgcgc tccctgagcg agctcgtctc tctctaccca
     1201 ccggaaatgc tcgaggcgtc ctgccagatc gccgaccgct gccgcttctc catggacgag
     1261 cttcgctacg agtatcccga agagctggtg cccccccacc tgactccggg tgagtacctg
     1321 cggcagctca cccttgccgg tgcggccgaa cgctggccgg acggcgtgcc cgcagacacc
     1381 ctcgcgctca tcgagcggga gctcacactc atccgcgaga tgcgctacga gtactacttc
     1441 ctcacggtgc aggacatcgt gcagtttgcc cgcagccagc agatcctctg ccagggccgc
     1501 ggctcggcgg ccaactcggc cgtctgctac tgcctgcaca tcaccgaggt ggatccctcc
     1561 cgcatgcacc tgctgttcga gcgcttcgtc tccaaggagc gcaacgagcc gccggatatc
     1621 gacgtggatt tcgagcacga gcgccgcgag gaggtcatcc agtacatcta caagcgttac
     1681 gggcgcgatc gcgccgctct ggccgcgacc ctgatcacct accgcccgaa aagcgccgtg
     1741 cgggacgtgg gcaaggcgct gggcatggac ctggatctgg tggacctgct ggccaaatcc
     1801 ctggcctggt gggaccgccg ggaggcgttg aacgagcgtt tcgccgccgc cgggctcgat
     1861 cccgacagcc ccgtggtcgg gcactttctc cgcttcgtcg cggagatcct cggtttcccc
     1921 aggcacctct cccagcacgt gggaggattc gttatctccc aggggccgct tgctcagctg
     1981 gtgccggtgg agaacgcggc catggcggat cgaaccatca ttcagtggga caaggacgat
     2041 ctggaagccc tggggctgct caaggtggac gtgctcggcc tcggcatgct caccgccatt
     2101 cgcaaggctc tggcgtcact gaacgcctac cggggcacgg acctggccgt cgccagaatt
     2161 ccccccgagg accccgagac ctaccgcatg ctgcagcggg gcgacagcgt aggcgtgttt
     2221 caggtggagt cccgggccca gatggccatg cttccgcggc tcaagcccca gcatttctac
     2281 gacctggtga tcgaggtggc catcgttcgg cccggcccca tt
//
LOCUS       DBNHOX010000278         5509 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1243664, whole genome shotgun sequence.
ACCESSION   DBNHOX010000278 DBNHOX010000000
VERSION     DBNHOX010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1243664"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="AC16UZ_06095"
     CDS             complement(<1..301)
                     /locus_tag="AC16UZ_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246291.1"
                     /translation="MSQELRPSELDELPENLRLAFLPLHKRAFGLAVGLTLGGFLFLV
                     TVVATLAPGPAPLLPLLGQYFPGYTVSWVGAVVGFAWATFAFFVAGWFSAWVRNLV"
     gene            complement(630..1688)
                     /locus_tag="AC16UZ_06100"
     CDS             complement(630..1688)
                     /locus_tag="AC16UZ_06100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6246292.1"
                     /translation="MSDRPELSVIVPVLDGRDVLRRSIAALLQSDLPRDRWELIVVDD
                     GSTDGSGECAEAHADQVLRIDGGPRGPGFARNAAAEVASGDILVFVDADVCVHREVLG
                     KFLRAFDGREDLGAVFGAYDDQPTVAGLLSQYRNLFHRYVHLKGAGETETFWAGCGAV
                     RRDLFRELGGFDVERYARPQIEDIELGYRIRSRGYRILLDPSIQGTHMKRWTLGGMVK
                     TDLLDRGVPWMLLMLEDGPGGAREATLNVGIAEKVKIALLGVATLLLAAAAALRDGRL
                     AALAIGCVAIIALLNLPVYGWFAERRSVWFAVRVLPLNLLYYFISGLAVLIALVRHVM
                     TSTSRQSETVLVGRGVDS"
     gene            complement(1738..3231)
                     /locus_tag="AC16UZ_06105"
     CDS             complement(1738..3231)
                     /locus_tag="AC16UZ_06105"
                     /inference="COORDINATES: protein motif:HMM:NF005548.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6246293.1"
                     /translation="MEGLETVPDVGDGRQVDRIRPGDHVVVVGGGPAGLTAAYVLSKK
                     GFKVTVFEGEHILGGISQTARYKGFRFDIGGHRFFTKIEPVERLWHEILGDEFIAVPR
                     LSRIHYRGTFFHYPLKPLNALFGLGLFNSIAVVLSYLKWHFWPYEREENFEEWVTNRF
                     GRRLYEIFFKTYTEKVWGIPCTEIRAEWAAQRIKGLSLARALLSAASVTKRPAKIKSL
                     ITHFQYPRLGPGQMWETCARHIREMGNEVHLEHSVDEVLCENGRVHAVRVRHQGGTTD
                     VSCDHVISTMPLRSLVRSLRPEAPERARRSAEGLRYRDFLVVALILDREDLFPDNWIY
                     VHTPGVKVGRIQNFNNWSRAMVPEEGRTCLGLEYFCFEGDGLWSAPDGELIDLARREL
                     AVLDVTGAAQVLDGAVVRMPKAYPIYDAQYSACVEGVRAHIEGIPNLHTVGRNGMHKY
                     NNQDHSMLTALMTVWNMEGAAHDVWAVNTDFEYHEEQRLESRAPVAG"
     gene            complement(3637..5430)
                     /locus_tag="AC16UZ_06110"
     CDS             complement(3637..5430)
                     /locus_tag="AC16UZ_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HIR6246294.1"
                     /translation="MAGILARFVVLDSDPRYRPWSGHLVDEGRWTEQARSLVLFGELS
                     LESTLARIHLLLAPLFQASSAGMFALFGVGTATARILGAASGGLIIVITLFMLRRRVS
                     GPALLVSLLILAFQPDLLSLSRVAIPEVPSLLFELLAFMVLVSTPRSARRALIGGLLF
                     AVAVGIKGTLVLVAPVFACVIWVASAPEDPVGPARRLVAFAAGLCAPALLVLGASLLV
                     EGSGTWSRLGGVAPSVLALLGPASAYGMVANMLYGALAGELAALYLPMWMVACLLLSL
                     GRVQGRCRELYLGSAAWAVGYLLLGCLLMYFPGRYRVHGFVPIAINLAAGLTLLREAG
                     WREIRESLEALPPPRRAALAVGLALPLAVLLASPGVATAGALGITLERLTHHVPLVVA
                     LLLALAALASRFWRLPSVPASLIALPFLVLAVQGSAVAAGVPHAGHWSPGGVSGISSW
                     AGFMLVAVMANWLLMRRVGPIKAVQAAALAYAIVLASTWVVALGAKVVAPSYTIVSAG
                     RALPEVIPEGSEIATLGAASLFLENDLRYRDGVDHQAQYLVVAFANAEDVLRHTDHFP
                     ERVRSFDLNLGENHAALVANWNEITVFRRAP"
BASE COUNT         1054 a         1906 c         1757 g          792 t
ORIGIN      
        1 tcacgaggtt ccgcacccag gcgctgaacc acccggccac gaagaacgcg aaggtcgccc
       61 aggcgaaccc gacgaccgcg ccgacccaag acacggtgta tcccggaaaa tactgcccca
      121 gcagaggtag gagcggcgcc ggaccgggag cgagcgtggc gacgaccgtc acaaggaaga
      181 ggaagccacc gagcgtcaac ccgacggcca ggccgaacgc cctcttgtgc agcggtagga
      241 acgcaaggcg gagattctcc ggcagctcgt cgagctcgga tgggcgaagc tcttgactca
      301 tggtttctcc cgatacgtac ccgcgtgcgt tcgccccatc gggatgcgcc cgttgcttcg
      361 cccaccccag cgcgcgcgat ctgggaccga gatctgtctg accctacatc tgactccgac
      421 gccacccgaa tcacatggcg ggcgccgcct cgtgccgccg agcccggagt cgggcccgcg
      481 gaacctatcc gtcgggtcaa gcgctgtcac ctgaccgact cccctgccga tgccgtcgga
      541 tcgaacgcgg aggccgcagc cggcgcacgc gggacggcgt ctcttctcag cacggatcct
      601 gtctctcgct tctccggcag gacgtcggat cacgagtcca ccccgcgacc gaccagcacg
      661 gtctccgact gtcggctcgt cgacgtcatg acgtgccgca cgagggcaat cagcaccgcg
      721 agaccgctga tgaagtagta aaggaggttg agcggcaaga cgcgtaccgc gaaccatacg
      781 ctgcgacgct cagcgaacca gccgtacaca gggaggttga gcagcgcgat gatcgccacg
      841 cacccgatcg cgagcgccgc gagacgcccg tccctcaagg ccgccgcagc cgcgaggagc
      901 agggtcgcga cgcccagcag cgcgatcttc accttctcgg cgatcccgac gttcagggtc
      961 gcctcacgtg cccccccggg gccgtcttcc agcatgagga gcatccacgg cacgcctcga
     1021 tcgaggaggt cggtcttcac catgccgccc agtgtccacc gcttcatgtg ggtcccctgg
     1081 atgctcggat ccaacaggat gcggtagccg cggctcctga ttcggtaccc gagctcgatg
     1141 tcctcgatct gcgggcgcgc atagcgctcg acatcgaagc cgccgagctc gcggaagaga
     1201 tcgcgccgga cggcgccgca tcccgcccag aacgtctccg tctcgcccgc ccccttcagg
     1261 tggacgtaac gatggaacag gttccggtac tgcgagagga ggccggccac cgtcggctga
     1321 tcatcgtagg cgccgaatac cgctcccagg tcctcgcgcc cgtcgaaggc gcgaaggaac
     1381 ttcccaagga cttcgcggtg cacgcagacg tcggcatcca cgaagacgag aatgtccccg
     1441 ctcgcgacct cggcggctgc attccgcgcg aagccgggtc cgcgcgggcc gccgtcgatc
     1501 ctcagcacct gatcggcgtg agcctcggca cattcccccg aaccgtcggt gctcccatcg
     1561 tccacgacga tcagctccca acggtctctc ggcaggtccg actggaggag ggccgcgatg
     1621 ctgcggcgaa ggacgtcgcg gccgtcgagg accggcacga tgaccgaaag ctcgggacga
     1681 tcactcaacg tcgcaccctc ccgactacgg gtggcgactc cggtctccgc tccggtctca
     1741 accggccacc ggcgcccggg actccagtcg ctgctcctca tgatactcga agtcggtgtt
     1801 cacggcccat acgtcgtggg cggcgccctc catgttccaa acggtcatca aggcagtgag
     1861 catggagtga tcctgattgt tgtacttgtg cattccgttc cttccgaccg tgtggaggtt
     1921 cgggattcct tcgatgtgcg cacgcacgcc ctccacgcac gcgctgtatt gcgcgtcata
     1981 gatgggatac gccttgggca ttcggacgac cgcaccgtcg agcacctgtg cggctcccgt
     2041 aacgtcgagg acagcgagct cgcgccgggc cagatcgatg agctctccgt ccggcgctga
     2101 ccagaggccg tcgccctcga agcagaagta ctccagcccc aggcaggtcc ggccctcttc
     2161 gggcaccatc gccctactcc aattgttgaa gttctggatc cgaccgacct tcacacccgg
     2221 agtgtgcacg tagatccagt tgtcggggaa tagatcttcg cggtccagga tcagggcaac
     2281 gacgaggaaa tcacggtagc gcagcccttc cgccgacctt cgagcgcgct cgggcgcctc
     2341 agggcgcagc gagcggacga gggaacggag cggcatcgtc gagatcacgt gatcgcagct
     2401 cacgtcggtc gtcccgccct ggtgccggac gcgcaccgca tgtacccgtc cattctcgca
     2461 caagacctcg tccacgctgt gctccaggtg gacctcgttg cccatctcac ggatgtgccg
     2521 tgcgcaggtc tcccacatct ggccggggcc gaggcgagga tactgaaagt gcgtgatcag
     2581 agacttgatc ttggccgggc gcttggtgac cgatgcggcc gagagcagcg cccgcgccag
     2641 agagagtccc ttgatgcgtt gggcggccca ctccgccctg atctccgtgc acgggatgcc
     2701 ccagactttt tcggtatacg tcttgaagaa gatctcgtag agccggcgac cgaagcgatt
     2761 cgtcacccac tcctcgaagt tctcctcacg ctcgtacggc cagaagtgcc acttcaagta
     2821 ggagaggacc acggcgatcg agttgaagag gccgaggccg aagagggcgt tcagcggctt
     2881 gagcgggtag tggaaaaagg tgcctcggta gtggatgcgc gagagccggg gcacagcgat
     2941 gaactcgtcc cccaggatct cgtgccagag ccgctcgacg ggctcgatct tcgtgaagaa
     3001 gcggtgcccg ccgatgtcga accggaagcc tttgtagcgc gcggtctgcg agattccacc
     3061 caggatgtgc tcgccctcga acaccgtgac cttgaagccc ttcttggaga gcacgtacgc
     3121 cgccgtcagc ccggcggggc cgccgcccac gaccacgacg tggtcgcccg ggcggatgcg
     3181 atcgacctgc ctgccgtcgc ccacgtccgg gacggtttcg agaccctcca tcggctcaac
     3241 cgtgcgcaca ggactcagca tgcgccggtg cccccgttgg aggccgcgcg ctctgcgagt
     3301 gggtgggtcg cggcatttac ggtccgatct cccatcagaa gtcgcagcct cgtcgcctcg
     3361 cggaagtccg caagctcgaa ccacagcggc atcgagctct ccccccctcc ctttcgaggg
     3421 gcgccatatc gagtgcgggc atgccggccc ggctactcgc accgtggagc ctgaccaaca
     3481 atgtgctgcg cccgctcatc gccgggggcg tcgcggaagc tcggatgcac tcgtcgtgac
     3541 cgctcgtcgg gctcgatcgg cccgcgccgg cacaatccga tagacatacg tacgtggcaa
     3601 gaatgatacc ctcacgaagg gtacacgacg aaaggctcac ggagccctcc ggaacacggt
     3661 gatctcgttc cagtttgcca ccaaggcggc gtggttctcg ccgaggttca ggtcgaagga
     3721 ccgcacgcgc tccgggaaat ggtcggtatg ccgcaagaca tcttcggcgt tcgcgaacgc
     3781 gaccaccaga tactgcgcct gatggtcgac cccgtcccga tacctgaggt cgttctccag
     3841 gaacagggag gcggcaccca gcgtggcaat ttccgatccc tcagggatca cctccggaag
     3901 cgcacggccc gctgatacga tcgtgtagct cggcgccacg actttggcac ccaaggccac
     3961 cacccaggtc gacgccaaaa cgatcgcgta ggcaagcgcg gcagcttgga ccgccttgat
     4021 cggtccgacg cgccgcatca gcagccagtt cgccatcacc gcgaccagca tgaacccggc
     4081 ccaggacgaa atcccggaca ccccaccagg actccagtga cccgcgtgcg gaacgccggc
     4141 cgcgaccgcg gacccctgca cggccagcac cagaaagggg agggcgatca ggctcgctgg
     4201 gacgctcgga aggcgccaga acctcgaggc aagggccgca agtgccaaga gaagcgccac
     4261 gacgagaggg acgtggtggg taagacgttc gagtgtgatg cccagagcgc cagccgtcgc
     4321 aaccccggga gaagcgagca ggacggcgag tgggagggcc aagcccaccg ccaacgctgc
     4381 gcgccgagga ggcggaagag cctcgagcga ctctcggatc tcccgccaac cggcctcccg
     4441 gagcagagtg aggccagccg cgaggttgat cgcgatcggc acgaagccgt ggacccggta
     4501 gcgaccggga aagtacatga gcaggcagcc gagcaagaga tacccgacag cccacgcggc
     4561 ggagcccagg taaagctccc tgcaccgtcc ctgcacccgc cccagggaga ggaggaggca
     4621 ggccaccatc cacatcggca ggtacagcgc cgccaactca ccggcgagag cgccgtagag
     4681 catgttcgcc accattccgt acgcgctggc cgggcccagc agggccagca cgctcggggc
     4741 cacgccgccc agccgggacc aggtcccgga gccttcgacg agcagcgacg ccccgaggac
     4801 caggagcgcc ggagcgcaca ggcctgcggc aaaggccacg agtcgtcggg cgggacccac
     4861 cggatcttcg ggggcactgg cgacccagat cacacaagcg aagaccgggg caaccaacac
     4921 caacgtgccc ttgatgccca ctgcgaccgc gaacagcagg ccgccgatca gtgcccttct
     4981 cgccgagcgc ggcgtggaaa ccaggaccat gaacgccaag agctcgaaaa ggagcgacgg
     5041 cacctccgga atcgccacgc gactcaggct gagcaggtcg ggctggaagg ccagaatcag
     5101 cagcgacacg agaagcgccg gaccgctcac ccgacgccgc agcatgaaga gcgtgatcac
     5161 gatgatcagg ccgccgctcg cggcgccgag gatccgggcc gtggccgtgc cgactccgaa
     5221 gagggcgaac atgccggccg aggacgcctg aaagagtggc gcgagcagca gatggatgcg
     5281 cgcgagcgtg ctctccaggc tgagctcacc gaacagcacg aggcttctag cctgctccgt
     5341 ccagcgcccc tcgtccacca ggtgacccga ccagggccga taccgggggt ccgagtcgag
     5401 taccacgaag cgggccagga tgccggccat gaccaatgcg acgacgacga tgtctcgtgc
     5461 gcgtacggag cgatcaagga gtggatcagc tttctccaat ccaatctgc
//
LOCUS       DBNHOX010000279         3266 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_602490, whole genome shotgun sequence.
ACCESSION   DBNHOX010000279 DBNHOX010000000
VERSION     DBNHOX010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_602490"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="AC16UZ_06115"
     CDS             complement(<1..819)
                     /locus_tag="AC16UZ_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR6246295.1"
                     /translation="MSVLVVADHDNAELKPVTLVVVAAATAIGGDIDILVAGEGCGAV
                     AETAAKIPGVNKVLLADNAAYGHQLAENVAPLVAEIGAGYSHILTAHTTVGKNFLPRV
                     AAVLDVAQISDIQGVVGPDTFERPIYAGNAVATVKSNDAIKVLSVRGTAFDPVAAEGG
                     SASIESVASAHDAGVSSFIGEELAKSERPELTAASIIISGGRGMQSGENFVLLEGIAD
                     KLGAAIGASRAAVDAGFVPNDMQVGQTGKIVAPDLYIAVGISGAIQHLAGMKDSK"
     gene            complement(816..1568)
                     /locus_tag="AC16UZ_06120"
     CDS             complement(816..1568)
                     /locus_tag="AC16UZ_06120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR6246296.1"
                     /translation="MKVLVPVKRVVDYNVKIRVKPDNTGVDLNNVKMSLNPFCEISVE
                     EAVRLKEAGTATEVVAVAVGGTVCQEQLRTCLALGADRAVLIETDQEPQPLGIAKALK
                     ALQERENADIVIFGKQSIDGDNAQTGQMFAALTGMPQGTFASELTVADGKAQIVREVD
                     GGLQTVSVTLPCVVTTDLRLNEPRYASLPNIMKAKKKPLDVVTPDELGVNVAPTVETL
                     SVKPPAERQAGIKVGSVEELVDKLKNEAKVIS"
     gene            1830..>3266
                     /locus_tag="AC16UZ_06125"
     CDS             1830..>3266
                     /locus_tag="AC16UZ_06125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017527398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6246297.1"
                     /translation="MERESMDFDVVIVGGGPSGLAAACRLMQLAESSGQELSVCLVEK
                     GSEIGAHILSGAVFEPTALNELFPDWEERGAPLDNPVTLDEVYYLVNEENRLKMPGFA
                     VPKVTHNHGNHAISLGNLCRWLGEQAENMGVNVFPGFAAAEILYGEDGAVAGVATGDM
                     GVGADGEHKATYAPGYELRGKYTIFAEGCRGHLGKQLMAKFDLQRDSATQHYAIGFKE
                     LWDVTPENHVPGKVVHTMGWPGRHLKGMFTGSYLYHLPNQQVSLGLIVDLSYSNPHVS
                     PFDEFQRWKHHPLIAETLSGGKRVSYGARAIVKGGLSALPKLSVPGALLVGDDAGFLN
                     MTKIKGSHTAMKSGMLSAEAVFESLSGGSAGGEDLSSFQEKFDSSWLHEELSAARNSQ
                     QAIHKFGTLAGAAFIWVDQTLFRGRLPFTLKDPTPDHATLKPAAQSRRIDYPKPDGVL
                     SFDKLSSVFLSNTNHEEDQPCHLVLADPA"
BASE COUNT          578 a         1086 c         1000 g          602 t
ORIGIN      
        1 cttgctgtcc ttcatgcctg caagatgctg gatggcgccg gaaatgccca cggcaatgta
       61 gagatcgggt gccacgatct tgccggtctg gcccacctgc atgtcgttgg ggacgaagcc
      121 ggcgtccact gcggctcggg acgcgccgat ggcagcgccg agcttgtcgg ctatcccttc
      181 gagcagcacg aagttctccc cgctctgcat gccgcgcccg ccggagatga tgatgctcgc
      241 cgccgtcagc tcaggacgct ccgatttggc cagctcttct ccaataaagc tggacacccc
      301 ggcatcgtgg gcgcttgcca cgctctcgat gctggccgag ccgccctcgg cggccacggg
      361 gtcgaaggca gtgccgcgca cggacagcac cttgatggcg tcgttggact tcaccgtcgc
      421 cacagcgttg cccgcgtaga tcggccgctc gaaggtatcc ggcccgacca caccctggat
      481 gtcggatatc tgcgccacgt cgagcacggc tgccacccgg ggcagaaagt tcttgccgac
      541 cgtggtatgg gcagtgagga tgtgggagta cccggcgcca atttccgcca ccagcggcgc
      601 gacgttctcc gcaagctgat gcccgtaggc agcattgtct gccaggagca ccttgttgac
      661 cccgggaatc ttggcggcgg tttcagccac cgcgccgcag ccttcacctg cgaccaggat
      721 gtcgatatcg ccgccgatgg ccgtcgcggc cgccaccacc accagggtga ccggcttgag
      781 ttccgcgttg tcgtggtctg caactaccaa tacgctcatg agattacctt tgcctcgttc
      841 ttcagcttgt cgaccagctc ctcgacgctc cccaccttga tacccgcctg ccgttcggcc
      901 ggcggtttga cggaaagcgt ctccacggtg ggagcaacat tcacccccag ctcgtccggc
      961 gtgacgacgt ccagaggttt cttcttggcc ttcatgatgt tggggagaga cgcgtagcgc
     1021 ggctcgttca gccgcaggtc cgtggtgacc acgcagggca gcgtgacgga gaccgtctgc
     1081 agaccgccat ccacctcccg cacgatctgc gctttgccat cggctacggt gagctccgag
     1141 gcgaacgtgc cctgaggcat cccggtcaga gcggcgaaca tctggccggt ctgggcgttg
     1201 tcaccatcga tgctctgctt gccgaagatc acgatgtcgg cgttttcccg ctcctgcagc
     1261 gccttgagcg ccttggcaat gccaagaggc tgcggctcct gatccgtctc gatgagcacc
     1321 gcacgatcgg ccccgagcgc caggcaggtt ctgagctgct cctgacaaac ggtgccaccg
     1381 accgccacgg cgaccacctc ggtagccgtt ccggcttcct tgaggcggac ggcttcttcc
     1441 acggaaatct cgcagaatgg gttcaacgac atcttgacgt tgttcaggtc gacgccagtg
     1501 ttgtcgggct tgacccggat cttgacgttg tagtcgacca cgcgttttac cggaaccagt
     1561 accttcatga attcctcgaa cggctaacaa gttaaacaga acagcaggtg ccgacgtttc
     1621 gacggctacc cggaggggcg cgtattctcc cttgggtcct caccgaggtc aagacgaagg
     1681 gggcccgagg atcggcagcg cagaaccggt gcgcgggtga aggccctccc ctataatatt
     1741 tgagctaata tttgagccca gcagcccgcc cggtcggggc ctttgaagct gcgacgcaaa
     1801 cccgcaaatc aaagcagtgg agagtcatcg tggaaagaga atccatggat ttcgacgtcg
     1861 tcatcgtggg cggtggtccc tccggcctgg cagctgcctg ccggctgatg cagctggccg
     1921 aatcatcggg tcaggagctg agcgtctgcc tggtggagaa aggctcggag atcggggcgc
     1981 acattctttc cggcgccgtt ttcgagccca ccgcgttgaa cgagttgttt cccgactggg
     2041 aggagcgcgg cgcgccgctg gacaaccccg tgacccttga cgaggtctac tatctcgtca
     2101 acgaggaaaa ccgcctgaag atgccgggct tcgccgtgcc caaagtcacc cacaatcacg
     2161 gcaaccacgc gatcagtctg ggcaacctct gccgctggct cggggagcag gccgagaaca
     2221 tgggcgtgaa cgtctttccc ggatttgccg ccgccgagat cctctatggg gaagacgggg
     2281 ccgtggccgg cgtcgccacg ggcgacatgg gcgtcggcgc ggacggcgaa cacaaggcaa
     2341 cttacgcgcc cggatacgag ctcaggggca agtacaccat cttcgccgag ggctgccgcg
     2401 gccatctcgg caagcagctg atggccaagt tcgatctgca gcgggattcg gccacccagc
     2461 actacgccat cggcttcaag gaactatggg acgtgacccc cgagaatcac gtgccgggca
     2521 aggtggtgca caccatgggc tggcctggca ggcacttgaa aggcatgttc accgggtcct
     2581 acctctacca cctgcccaac cagcaggtgt ctctcgggct gatcgtggac ctgtcctaca
     2641 gcaatcccca cgtgagcccg ttcgacgagt tccagcgctg gaagcaccat ccgctcatcg
     2701 ccgagaccct cagcgggggc aagcgcgtct cctacggtgc ccgggccatc gtcaagggcg
     2761 gcctgtccgc gctgcccaag ctgtcggtgc ccggcgcgct gctggtgggt gacgatgccg
     2821 gctttctcaa catgaccaag atcaagggca gccacaccgc catgaaatcg ggcatgctct
     2881 cggccgaagc cgtcttcgag tccctttccg gcggttccgc gggcggcgag gacctgtcca
     2941 gcttccagga gaagttcgac tcgtcctggc tgcacgagga gctttcagcg gcgcggaatt
     3001 cccagcaggc catccacaag ttcggcaccc tcgccggggc cgcgttcatc tgggtggacc
     3061 agaccctgtt tcgcggcagg ctgccgttca ctctgaagga tcccacgccc gaccacgcca
     3121 cgcttaagcc ggccgcccag agcaggcgga tcgactaccc caagcccgac ggcgtgcttt
     3181 ccttcgacaa gctgtcctcc gtctttctct cgaacaccaa tcacgaggaa gaccagccct
     3241 gccacctggt gctggccgac ccggcc
//
LOCUS       DBNHOX010000280         2944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1694861, whole genome shotgun sequence.
ACCESSION   DBNHOX010000280 DBNHOX010000000
VERSION     DBNHOX010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1694861"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="AC16UZ_06130"
     CDS             <1..1010
                     /locus_tag="AC16UZ_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HIR6246298.1"
                     /translation="GAPFMYDIYGLGNALVDMEYRVDDGFLRRHAITKGHMTLVEEDR
                     LDALIADLDDYDPERMSGGSAANTVIAAQCFGVSTFYSCKVAGDATGHYFLRDMGTAG
                     VNTNANANDGPGKSGRCLVLVTPDAERSMNTFLGVSSQLSTAEIDEAALAKAGFFYVE
                     GYLSSSPGSLEAAVECRQLAEDSGVKTAVSLSDPSMVEFFRDGMEQILGNGVDHLFCN
                     EEEALAWSRTDRLDIAIRELAEVARAVNITLGARGSLAVNGREQRRAPGYPVTAVDTN
                     GAGDIYAGACLFGWAAGMSSEQAAAFGNFAAANLVQRYGARFRHAGDYQSVLGDFRKV
                     S"
     gene            complement(1003..1704)
                     /gene="sfsA"
                     /locus_tag="AC16UZ_06135"
     CDS             complement(1003..1704)
                     /gene="sfsA"
                     /locus_tag="AC16UZ_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HIR6246299.1"
                     /translation="MNFHPPLEVARLERRYKRFLADLLTDQGPLTVHCPNTGAMLGCS
                     DPGSEVWYSDSGNERRKYRHTLEVVCTPTGRVGINTARANRLVEEAIARGGVAELDGY
                     GQIRREAAIPESKGRFDFLLEGARGRCFVEVKSMTHNLGRGRGAFPDTVSERALRHVE
                     ALIGRKRAGDRAVLLFCVQHTGIDLATTADAIYPEYGEAVRRAVSEGVEVIAYGCRID
                     RDALAVGEALPVELS"
     gene            complement(1701..2873)
                     /locus_tag="AC16UZ_06140"
     CDS             complement(1701..2873)
                     /locus_tag="AC16UZ_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007159703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6246300.1"
                     /translation="MYAQRLDRIAPFRVMQLMERAKAIEASGHRVVHFEVGEPDFVTA
                     DPIVRAGIDALEAGETKYTQALGIPELRERIAQHYCQELGVSLSADRVVVTSGASGGL
                     LLLSGLLLNPGDRLLLTDPGYPCNEVFAGLVNAEPERIPLSADRGFQLHADDLEAHWG
                     PRARGLLLASPANPTGAVISRAHLAELVAALARRGGWLILDEIYQGLIYGDGPGYRTG
                     LEITDEAFILNSFSKYYGMTGWRLGWMVVPEAALEPVARLAQNLFISPSSIAQRAALS
                     AFGPEARAIHEQRRAAFEGRRDALREGLTELGLRVPVVPGGAFYLYVDVSPIGIDSAE
                     FCWRLMDDYHVAVTPGIDFGDHLADQYVRFAYTTDEDSIALGLERIETALKDMGAL"
BASE COUNT          547 a          979 c          942 g          476 t
ORIGIN      
        1 ggggagcccc gttcatgtat gacatctacg gcctgggaaa tgccctggtg gacatggagt
       61 atcgcgtgga tgacggtttt ctgcgccgtc atgccatcac caaaggacac atgacgctgg
      121 tggaggaaga ccggctcgat gctctgatcg ccgacctgga cgactacgac ccagaacgca
      181 tgagcggtgg atccgcggca aacacggtca tcgcagccca gtgcttcggc gtaagcacct
      241 tctactcctg caaggtcgcc ggcgacgcca ccggccacta ctttctgcgg gacatgggca
      301 cggcgggcgt gaacaccaac gccaatgcca acgacggacc gggcaagtcg gggcgttgcc
      361 tggtgctggt cacacccgac gccgagcgat ccatgaacac ctttctcggc gtgtcgagcc
      421 agctcagcac agccgagatc gacgaggcgg cactggccaa agcaggcttc ttctacgtcg
      481 agggctacct gagctccagt ccgggaagcc tggaggcggc cgtggagtgc cggcagcttg
      541 ccgaggactc gggcgtgaaa accgcggtaa gcctctccga tcccagcatg gtcgagttct
      601 tcagggacgg catggagcag attctgggca acggcgtgga ccatttgttc tgcaacgagg
      661 aagaagcact ggcgtggtcg cgaacggacc ggctggacat cgccatccgc gagctcgccg
      721 aggtggcccg ggcagtgaac atcaccctgg gcgcccgggg cagcctggcg gtgaacggcc
      781 gtgagcagcg ccgcgcgccg ggctacccgg tgacggccgt cgacaccaac ggtgccggag
      841 acatctacgc cggtgcctgc ctgttcggct gggcggccgg catgtcctcg gagcaggcgg
      901 ccgccttcgg aaacttcgcg gcggctaacc tggtacagcg gtacggtgcc cgattcaggc
      961 acgcaggcga ctaccagagc gtgcttggcg acttccggaa ggtcagctga gctccacggg
     1021 gagcgcctcg cccaccgcaa gggcatcccg atcgatccga cacccgtagg cgatcacctc
     1081 gacaccttcg gagacggccc gacgcaccgc ttcaccgtac tcgggataga tcgcatccgc
     1141 cgtggtcgcc agatcgatgc cggtgtgctg gacgcagaac agcagcacgg cgcggtcacc
     1201 ggcccgcttc ctgccgatca gcgcttcgac gtggcgcaac gcccgttcgc tcaccgtatc
     1261 cgggaaggcg ccgcgccccc ggccgagatt gtgggtcatg ctcttgacct cgacgaagca
     1321 gcgccccctg gcgccctcga ggaggaaatc gaagcgaccc ttgctttcag ggatggccgc
     1381 ttcgcgcctg atctgcccgt acccgtcaag ctcggccacg ccgccccggg caatggcttc
     1441 ttcgaccagc cgattggctc ttgccgtgtt gatccccacc cggccggtcg gcgtgcacac
     1501 cacctcgagc gtgtgacgat acttgcggcg ctcgttcccg gagtctgaat accagacctc
     1561 gctgccggga tctgagcatc caagcatggc gccggtgttg gggcagtgca cggtcagcgg
     1621 accctggtcg gtcaacaggt cggcgagaaa tcttttgtag cgacgctcca ggcgagcaac
     1681 ttccagcggc gggtggaaat tcacagggcg cccatgtcct tgagcgccgt ctcaatccgc
     1741 tcgagcccga gggcgatgga gtcttcgtcg gtggtgtacg cgaaccgcac gtactgatcg
     1801 gccagatggt caccaaaatc gatccccggc gtaaccgcta cgtggtaatc gtccatcagg
     1861 cgccagcaga actcggccga gtcgatgccg atgggcgaga catccacgta gagatagaac
     1921 gccccgcccg gcaccacggg gactcgcaga ccgagctccg tcaggccctc gcgcagcgcg
     1981 tctctgcgcc cctcgaaggc agcacgtcgt tgttcgtgaa tcgcccgcgc ctccggcccg
     2041 aaggccgaca atgccgcccg ctgggcgatg gacgacggcg agatgaagag attctgggcg
     2101 agcctggcaa cgggctccag cgcggcctcc ggaaccacca tccacccgag ccgccagccg
     2161 gtcattccgt agtacttgga aaagctgttg agaatgaatg cctcgtcggt gatctcaaga
     2221 ccggtgcggt atccgggccc gtcaccgtag atcaggccct ggtagatctc gtcgaggatc
     2281 agccagccgc ctctacgagc cagggcagca acgagctccg cgagatgcgc ccgggagatc
     2341 accgcccccg tggggtttgc cggcgaagcc agcagcaaac cccgcgctct gggcccccaa
     2401 tgcgcctcca gatcgtcagc gtgcagctga aagccacggt cagcggacag cggaattcgc
     2461 tcgggctcgg cgttgacgag cccggcgaac acctcgttgc acggatatcc ggggtcggtg
     2521 agcagcaggc ggtcacccgg gttcaacagc aacccgctca gcaggagcag accaccgctg
     2581 gcgcccgagg tgaccacgac ccggtcggcg gagagagaaa caccaagttc ctgacagtaa
     2641 tgctgggcga tgcgctcacg cagctccggg atgcccagcg cctgggtgta ctttgtttcc
     2701 cctgcctcca gggcatcgat gcccgcccga acgatggggt cggcggtcac gaagtccggc
     2761 tcgcctacct cgaagtgcac gacgcggtgc cccgaagcct cgatggcctt cgcccgttcc
     2821 atcaattgca tgacccgaaa cggggctatt cggtccagcc gctgcgcgta catgtgccct
     2881 gaattctccc tttgagccgt gcgtcagaaa tcacgaaaaa cctggacgga ttatatggtc
     2941 aacg
//
LOCUS       DBNHOX010000281         2671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1501958, whole genome shotgun sequence.
ACCESSION   DBNHOX010000281 DBNHOX010000000
VERSION     DBNHOX010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1501958"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="AC16UZ_06145"
     CDS             complement(<1..443)
                     /locus_tag="AC16UZ_06145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR6246301.1"
                     /translation="MEITPGSRILFVLPGGPLGDLSVSATIVNTWIVMAILVIGGRIA
                     TLGIGPATPTSHWRLAVELLIEMIRNQIRAIGGGDPSRYLPFVGTLFLFTLVSNVLGV
                     VPGFISPTGSLSTAVALALCVLVAVPMYGIAETGLFNYLRRYIRPT"
     gene            complement(448..723)
                     /locus_tag="AC16UZ_06150"
     CDS             complement(448..723)
                     /locus_tag="AC16UZ_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246302.1"
                     /translation="MSGDLMLGLLSGLVLGALHLLWLQRASARLGAGAGVGALLAGAA
                     LRLAVVLAGFAAVAWVAMQPGLALIAALGGFALARTLGLWHVRNRAR"
     gene            complement(732..1040)
                     /locus_tag="AC16UZ_06155"
     CDS             complement(732..1040)
                     /locus_tag="AC16UZ_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR6246303.1"
                     /translation="MSGPDKDLEETVGRKAERRDRARREGRHTVWFGFGMLGLVGWAV
                     AVPTLAGVALGHWLDARYPAGPSWTITGLVAGVALGCLNAWWWVQRAGGRGDGDADAR
                     "
     gene            complement(1037..1441)
                     /locus_tag="AC16UZ_06160"
     CDS             complement(1037..1441)
                     /locus_tag="AC16UZ_06160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HIR6246304.1"
                     /translation="MSAPSEHMRLEVFTPTALLVDDAAVKVIAEGPEGYFAVLPRHID
                     LAAALTPGLLVYVTAAGEERLLGIDEGVLVKRGAEVRVSVLDAFESDDLASLRKQVAR
                     RFLSLDEHERSARTALARLEAGAIRHVLEIER"
     gene            complement(1438..>2671)
                     /gene="atpD"
                     /locus_tag="AC16UZ_06165"
     CDS             complement(1438..>2671)
                     /gene="atpD"
                     /locus_tag="AC16UZ_06165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014869554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR6246305.1"
                     /translation="GLARGLVLSTGQPVTLGARLRATGGGIEMPVGRTTLGRMLNLFG
                     EPVDGGPPLTDAPRRSIPHAPPRLSERRTRSEIFETGIKAIDLLCPIERGGKAGLFGG
                     AGVGKTVLIAEMIYNMLGVYQGVSLFCGIGERSREAEEFHREMEEAGVLANTVMVFGQ
                     MNERPAVRFRVGHAAMTIAEHFRDELNTDVLVLIDNIYRFVQAGAEVSGLLGQIPSRV
                     GYQPSLATEIAELEERICSTPSGAMTSIQAVYVPADDFTDPAATHVFGHLSASVVLSR
                     KRAAQGLYPALDPLESASKMLTPGVATAQHESVARDVRRTLAEFESLKDVIAMLGLEQ
                     LSVQDRRTVAVARRLERFLTQPFHATQQFTNAPGRRVSLEETIEGCALILSGAFNDRP
                     ERHLYMIGAIDEAGKAEE"
BASE COUNT          512 a          941 c          810 g          408 t
ORIGIN      
        1 gttgggcgga tatagcggcg cagatagttg aacaggccgg tttcggcaat cccatacata
       61 ggaactgcca ccaggacgca gagagcgagc gcgacggcgg tggacagcga gcccgttggc
      121 gaaatgaagc cgggaacgac gcccagcacg ttcgacacaa gcgtgaacaa gaacagggtt
      181 ccaacgaagg ggagatagcg cgacggatca ccgccgccga tagcgcggat ctggttgcgg
      241 atcatctcaa tcaacagttc gaccgccagc cgccagtgcg acgtcggcgt ggcggggcca
      301 atcccgagcg tcgcgatgcg accgccgatc accaggattg ccatcacgat ccatgtgttt
      361 acgatcgtcg ccgagacaga cagatcgcca agcgggccgc ctggcagcac gaacaggatc
      421 ctgctgcccg gggttatctc catgccctca cctcgcccta ttccgaacgt gccacagtcc
      481 cagcgttctc gccagggcaa agccgccaag cgcggcgatt agggccagac ccggctgcat
      541 cgccacccac gcaacggctg caaagcccgc cagcacgaca gccaagcgca atgcagcccc
      601 ggcgagcaga gcgcctacgc cggccccagc gccgagccgc gccgaggcac gctggagcca
      661 cagcagatgc agcgcgccga gcaccagccc cgaaagtaac cccaacatga gatcgccgct
      721 catacccgcg gtcatcgggc gtctgcgtcg ccatcgccgc ggcccccggc tcgctgcacc
      781 caccaccagg cattcagaca gccaagcgcc acgccggcca cgagccccgt tatcgtccaa
      841 gaggggcccg cggggtagcg cgcatccagc caatgcccca gcgccacacc cgcgagcgtg
      901 gggaccgcca ccgcccatcc caccagccct aacatgccga aaccgaacca gaccgtgtgc
      961 cggccctctc tgcgcgcccg gtcccgccgt tcggccttgc ggccgaccgt ctcttccagg
     1021 tccttgtctg gcccgctcat cgttcaatct ccaggacgtg gcggattgct ccggcttcga
     1081 gcctggcgag cgcggtgcgg gctgagcgct cgtgttcgtc gaggctaagg aaccgacgtg
     1141 ccacttgttt ccgcaacgac gcgagatcat cgctctcaaa ggcatcgagg accgaaacgc
     1201 gcacctccgc cccacgcttg accagcacgc cctcgtcgat gcccagcagg cgctcttctc
     1261 ctgcagcagt gacatatacg agcaggcccg gtgtcagcgc cgcggccagg tcgatgtgac
     1321 ggggcagaac agcgaagtac ccctcaggcc cctcggcgat caccttgacg gccgcgtcat
     1381 ccaccaacag cgcggtcgga gtgaacacct ccaggcgcat gtgctccgac ggcgcgctca
     1441 ttcctcggcc ttcccggctt cgtcgatcgc accgatcatg tatagatgac gctccggccg
     1501 atcgttgaac gcgccggaca gaattagcgc gcagccctcg atcgtctcct ccaggctcac
     1561 ccgacggccc ggcgcgtttg tgaactgctg ggtggcgtga aagggctgag tcaggaaccg
     1621 ctccagccga cgcgcaacgg cgaccgttcg gcgatcctgg accgacagct gctcgagtcc
     1681 cagcatggcg atcacgtcct tgagactctc aaattccgcc agcgtgcggc gcacgtcgcg
     1741 ggcgacactt tcgtgctggg cggtcgcaac gccgggggtc agcatcttcg aggcggattc
     1801 cagaggatcg agggccgggt aaagcccctg cgcagcccgc ttgcgcgaca gcacgaccga
     1861 agccgaaaga tggccgaaga catgggttgc agcgggatcg gtgaagtcgt cggccggcac
     1921 gtagaccgcc tgaattgaag tcatggcacc cgagggcgtg gagcagattc gctcctccaa
     1981 ttccgctatc tcggtagcga gcgagggctg atagccgacc ctcgatggaa tctggcctag
     2041 gagacccgag acctctgccc ccgcctggac gaagcgataa atgttgtcga tcaacaccag
     2101 tacgtcagtg ttgagctcat cgcgaaaatg ctccgcaatc gtcatggcgg cgtgaccaac
     2161 gcgaaagcgt accgcggggc gttcgttcat ctggccgaat accatgacgg tgttagccag
     2221 gacgccagcc tcttccattt cgcggtggaa ctcttccgcc tcgcgcgagc gttcgccaat
     2281 gccgcagaac aggctcacgc cctgatacac gcccaacatg ttatagatca tctcggcgat
     2341 cagcacggtc ttgccaacgc cagccccgcc gaagagaccc gccttgccac ctcgctcgat
     2401 gggacagagt aggtcgatcg ctttgatgcc ggtctcgaat atctcggacc gcgtccgtct
     2461 ttcgctcagc cggggcgggg cgtgcgggat cgagcgccgc ggcgcgtccg taagcggcgg
     2521 gccgccatcg accggctcgc cgaacagatt cagcattcgg ccgagcgtcg tccgccccac
     2581 cggcatctcg attcctcccc cggttgcgcg cagtcgggct ccgagcgtca ccggctgccc
     2641 ggtagacagc acaagccctc gggccagccc c
//
LOCUS       DBNHOX010000282         5558 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_409124, whole genome shotgun sequence.
ACCESSION   DBNHOX010000282 DBNHOX010000000
VERSION     DBNHOX010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_409124"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..2069)
                     /gene="parE"
                     /locus_tag="AC16UZ_06170"
     CDS             complement(177..2069)
                     /gene="parE"
                     /locus_tag="AC16UZ_06170"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIR6246306.1"
                     /translation="MADASKYSAESLQVLSGLEPVRRRPGMYTDTSRPNHLVQEVVDN
                     SVDEALAGYAREIEVTLFKDGSVEVVDDGRGMPVDVHPKHKVSGVELILTRLHAGGKF
                     DNENYSFSGGLHGVGVSVVNALSEWLEVEVKREGRVFRQRYKGGKPTGKLKPEGTVGQ
                     RNTGTRVTFKPDAGYFDSVTVAVGRLKHLLRAKAVLCPGLRVSLNVEGKESESWHYEN
                     GLEGYLVNALAGAEVIPEKPFMHAATGNQEGVDWAVQWVLDGGELITESYVNLIPTAQ
                     GGTHVNGLRSGLIDALKEFCEFRDLLPRGVKLTGDDLWDQCSYVLSAKLKDPQFSGQT
                     KEKLSSRQSAVFIGGVAKDAFSLWLNEHPQDGERLAELAISNAQKRVQAAKKVARKKV
                     TAGPALPGKLADCSGQDAERAELFLVEGDSAGGSAKQARDREFQAVMPLRGKILNTWE
                     VDVSQVLDSQEVHDIALAIGVDPGSPDLSGLRYAKVCILADADSDGLHIATLLCALFL
                     RHFRALVDAGHVFVAMPPLYRIDVGKEVFYALDEAEKEGVLDRIAAEKKRGQIQVQRF
                     KGLGEMNPMQLRETTMNPDTRRLVRLELDSPTKTDEVMDMLLAKKRSPDRRAWLEKKG
                     DQASLA"
     gene            complement(2087..2884)
                     /locus_tag="AC16UZ_06175"
     CDS             complement(2087..2884)
                     /locus_tag="AC16UZ_06175"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HIR6246307.1"
                     /translation="MKTVLHITDLHLQPRESDRLLGVDTADSLRAVLARALAEGRPDA
                     LLVTGDVSHEGDEASYRRFAAIVADHYEGPVLCLPGNHDKSAAMREVLGETWALDLQE
                     WSLIGLDSHVDGQVGAGLSETDMAALEGRLAASRGRHVLVATHHPPVDVGCPWLDKDR
                     IQNGAELLEWLSEHAGVKGMVFGHAHQVIESAYENLALLGTPATCFQFAPRTERFAVD
                     ERKPGYRWLRLDAQGGLQTEVGRVDDYPLTLDLSERSRSAGRAPKPN"
     gene            complement(2943..3347)
                     /locus_tag="AC16UZ_06180"
     CDS             complement(2943..3347)
                     /locus_tag="AC16UZ_06180"
                     /inference="COORDINATES: protein motif:HMM:NF018552.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HIR6246308.1"
                     /translation="MAECDANYLRLMKLFPSLREEDVSDFAVCIGDMHYEVCIEVQDR
                     GPYTTLVRLSQLPEAPWSTRPALTVRVYHDARSAEVVEYQRGGHFQAVYDYPNRDMRQ
                     PDEKAQVNRFLGEFLAMCLTHGVSVREPEFAS"
     gene            3629..5017
                     /locus_tag="AC16UZ_06185"
     CDS             3629..5017
                     /locus_tag="AC16UZ_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HIR6246309.1"
                     /translation="MNKPRLIIALLGLAWASAASATDILDAYRDAQSNDPVLGAARAG
                     LDATQELIPQSRSALLPRVAIGGQTSWNERSFPSPVFIEDIGTRVRIPDQEFNEHGWQ
                     AELRQPLFDLEAWFNFRSSQAGVEGAEWGFVSVEQDLIVRTVVAYLNVLRAQDLLDSA
                     KAAEDAVKRQLEQVQQRFDVGLVAITDVLEAQAVYDSAQVVRIQAAGDHDIFFETLRT
                     LTGQPYDSIARISEALPVVDPTPGNEEEWVNTALTGNPTILAANSQLDAAERTLKARR
                     SGHMPTVDATVSTNHFVTGGASFLGGKIDQDVYALSITLPIYQGGLTSSRAREARALA
                     EQARQELLDRQLTVSRDTRNLFRAVATDVVRVRARKKAIESSKSALEATQTGYEVGTR
                     NIVDVLDAQNRLYASEFDYADSRYNYIIDLMQLRQTAGTLNGEDVEQLNVYADSNDPV
                     ERLNSLKARVIN"
     gene            complement(5010..>5558)
                     /locus_tag="AC16UZ_06190"
     CDS             complement(5010..>5558)
                     /locus_tag="AC16UZ_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the transfer of
                     2-keto-3-deoxy-D-manno-octulosonic acid to lipid A;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HIR6246310.1"
                     /translation="AHDAVRSACPGACLLLVPRHPSWAADLEKMVQEAGFSVVRQSVA
                     AGAEAGVTVDVVIGDVMGQLLYLYGLSQAAFIGGSLVPVGGHNPIEPALCGQPLLTGP
                     HTFNFPDVVAAFEDSAALLRVGDAAGLAAEVARCLGDAELRERMAASARRVVAANTGA
                     TERLLALLREHVGRVSGGGPLS"
BASE COUNT          982 a         1890 c         1726 g          960 t
ORIGIN      
        1 ggcttgagcc cgtcggcaat gtggggcaac gcgcgatcgt tgatgacata catggcgtag
       61 tcgagatacg cccgctccgt ataggcccgc aggggtatgg tttctatgcc gggattttcc
      121 gtttccatgc tttccatggc gccatctggc gcctccaaat tgcggttgtt cgaaggctac
      181 gcgaggctcg cctggtcgcc cttcttctcc agccaggcgc ggcgatcggg cgaacgcttc
      241 ttggccagaa gcatgtccat gacctcgtcg gtcttcgtcg gtgagtcgag ttcgagccgg
      301 accaatcgac gggtgtcggg attcatcgtc gtctcgcgca gctgcatcgg gttcatctcg
      361 ccaagcccct tgaatcgctg cacctgaatc tgcccgcgtt tcttctccgc cgcgatccgg
      421 tccagcacgc cctctttctc cgcctcgtcc agggcgtaga agacttcctt gcccacgtcg
      481 atccggtaaa gcggcggcat ggcgacgaac acgtgtccgg cgtcgaccag cgcgcggaag
      541 tggcgcagaa acagcgcgca gagaagcgta gcaatgtgca ggccgtccga gtccgcgtcc
      601 gcgaggatgc agaccttcgc gtagcgcagg ccgctcaggt ccggcgagcc cggatccacc
      661 ccgatggcca gggcgatgtc gtgcacctcc tgggagtcga ggacctgact gacatccact
      721 tcccaggtat tgagtatctt gccgcgcagc ggcatgaccg cctggaactc tcgatcccgc
      781 gcctgcttgg cggagccgcc ggccgaatct ccttcgacaa gaaagagctc ggcccgctcg
      841 gcgtcctgac cggagcagtc cgccagcttc ccgggcagag cgggacccgc agtgaccttc
      901 ttgcgcgcga ccttctttgc cgcctggacc cgcttctgcg cgttggagat ggccagctcc
      961 gccagccgct cgccgtcctg cgggtgctcg ttgagccaga gactgaaggc gtccttggcc
     1021 acgccgccaa tgaaaacggc cgactggcgg gaagagagct tttccttggt ctgcccggaa
     1081 aactgcgggt ctttgagctt ggcggagagc acgtagctgc actgatccca gagatcgtcg
     1141 ccggtgagtt tgaccccccg aggaagaaga tcccggaact cgcagaattc cttgagcgca
     1201 tcgatgaggc ccgaacgcag accgttgacg tgcgtccctc cctgagccgt ggggatcagg
     1261 ttgacgtagg actcggtaat cagctcgccg ccatcgagca cccactgcac ggcccagtcc
     1321 acaccttcct ggttgccagt ggcggcgtgc atgaacggct tctcgggaat tacctcggca
     1381 ccggcgagcg cgttaaccag gtaaccttcc agaccgttct cgtagtgcca ggactcggat
     1441 tccttgcctt ccacgtttag gctcacgcgc agacccggac agagcacggc cttggcccgc
     1501 agcagatgct tgagcctgcc aacggcgaca gtgacggagt cgaagtatcc ggcatcgggc
     1561 ttgaacgtga cccgggtccc cgtgttgcgc tgacccacgg tcccctcggg tttcagcttg
     1621 ccggtgggct tgccgccctt gtagcgctgg cgaaagaccc tgccttcgcg ctttacttcc
     1681 acctccagcc attcggagag agcgttcacc acggaaacgc ccacgccatg caggccgccg
     1741 gagaagctgt agttctcgtt gtcgaatttg ccgccggcgt gaaggcgggt gaggatcagc
     1801 tccaccccgg aaaccttgtg cttcggatgg acgtccacgg gcatgccgcg gccatcgtcg
     1861 accacctcca cggagccatc cttgaacagc gtgacctcga tttcccgcgc atatccggcg
     1921 agcgcctcgt ccacgctgtt gtccaccact tcctggacca gatggttcgg ccgggaggta
     1981 tcggtgtaca tccccgggcg gcggcgcacg ggctcgagcc cggagagcac ctgcagcgat
     2041 tcagcggaat acttgctggc gtcagccatg aacggattgg acctcttcag ttgggtttgg
     2101 gggcccggcc agcgcttcgg ctgcgctcgg acaggtcgag tgtgagcggg taatcgtcga
     2161 cccgacccac ctccgtctgc aggcctccct gtgcatccag gcgcagccag cggtatcccg
     2221 gcttgcgctc gtccacggcg aacctctccg tgcgcggagc aaactgaaag catgtggccg
     2281 gcgtgcccag cagagcgagg ttttcgtagg cagactcgat gacctgatgt gcgtggccga
     2341 acaccatgcc ttttacaccg gcgtgctcgg acagccattc tagcagctcg gcgccgtttt
     2401 ggatgcggtc tttatcgagc cagggacatc ccacgtccac cggaggatgg tgcgtggcga
     2461 cgagcacgtg gcgccctcgc gatgccgcaa gccggccctc gagcgccgcc atgtccgtct
     2521 ctgaaagccc cgcgcccacc tgtccgtcca catgactgtc cagcccgatg agcgaccact
     2581 cctgcagatc gagcgcccac gtctcgccca gcacctcccg catcgcagcg cttttgtcat
     2641 ggttaccggg caggcagagc accgggccct cgtagtgatc ggcgacgatc gccgcgaagc
     2701 gccggtagct ggcctcatcc ccttcgtggc tgacgtcgcc cgtgaccagc agagcgtccg
     2761 ggcggccctc cgccagggct cttgccagga cggcacgaag gctgtccgcg gtgtccacgc
     2821 ccagcaaccg gtcgctttcc cggggctgca gatgcaggtc ggtaatgtgg agaacggttt
     2881 tcaggtttcg atggccgcgc ggccgacgtc gggctccggg ggggtgcgac ttccggagcc
     2941 gctcagctgg cgaactccgg ttcgcgcacg gaaaccccgt gagtcaggca catggcgagg
     3001 aactcaccca ggaatcgatt cacctgcgcc ttctcgtcgg gctggcgcat gtccctgttc
     3061 ggataatcgt agaccgcctg aaagtggccg ccgcgctgat actccaccac ctccgcgctg
     3121 cgggcatcgt gatagacacg aacggtgagc gccggccgcg tgctccaggg cgcttcgggt
     3181 agctggctga gccgcacgag cgtggtgtac gggcctcgat cctgtacctc gatgcaaacc
     3241 tcgtagtgca tgtcgccgat gcagacggca aagtcgctca cgtcttcctc gcgcagggag
     3301 gggaagagct tcatcagccg cagatagttg gcatcgcatt cagccatgtg tgcgggcaaa
     3361 tcaatgctgt aacgtttgtc gggcactgtc cacgaaaagc cggccatttc acctgccaat
     3421 ataccgacgc cgcgcggagg cgtcaccgtt gacagcgctg cgacggctaa tttaaggcaa
     3481 cggcccacaa ttccaggggc agatggctca gggatgggaa ccagttcaac gccccgccca
     3541 accgtccgac gatcagctcc gcggtgcttt gcagggtgcg tttgctaata tgcgcgctct
     3601 cttcctaaca gcaaagacgg acattgcctt gaacaaaccc agattgatca tcgccctgct
     3661 gggcctggcg tgggcctctg ccgcgagcgc taccgatatc ctggacgcgt accgggacgc
     3721 acagagcaac gaccccgttc tgggcgctgc ccgtgccggc ctggatgcaa cccaggaact
     3781 gataccccag agccgttcgg cgctcctgcc cagagtcgcc atcggcgggc agacgtcctg
     3841 gaacgagcgg agcttcccgt cacccgtctt tatcgaggac attggcaccc gggtacggat
     3901 tcccgatcag gaattcaacg agcacggctg gcaggccgag ctgcgccagc cccttttcga
     3961 cctggaagcc tggttcaact tccgcagctc tcaggccggc gtcgagggcg ccgaatgggg
     4021 cttcgtgtcg gtggagcagg atctgatcgt gcgcaccgtg gtcgcctacc tcaacgtcct
     4081 gcgggcccag gatctgctgg actccgcgaa agcggcagag gacgcggtga agcgccagct
     4141 ggagcaggtt caacagcgct tcgacgtggg cctggtggcc atcacagacg tgctggaagc
     4201 acaggccgtc tacgacagcg cccaggtggt ccggatccag gccgcgggcg atcacgacat
     4261 cttcttcgaa accctgcgca ccctcaccgg acagccttac gacagcatcg cgcgcatctc
     4321 ggaagcgctg cccgtggtcg acccgacgcc gggcaacgag gaggagtggg tgaacaccgc
     4381 attgaccggc aacccgacca tcctcgcggc caactcccag ctcgacgccg cggagcgaac
     4441 cctcaaggcg cggcgctcgg ggcacatgcc cacggtggat gccacggtga gcaccaacca
     4501 tttcgtcacc ggcggtgctt cctttctcgg gggcaaaatc gatcaggacg tctacgcgct
     4561 gtccatcacc ctgcccatct accagggcgg cctgacctcg tcgcgtgcgc gggaggctcg
     4621 ggctctggcc gagcaggcgc gtcaggagct gctggaccgg cagctgaccg tgagccggga
     4681 tacccgcaac ctgttccgcg cggtcgccac cgacgtggtc cgggtccggg cgcgcaagaa
     4741 ggccatcgag tccagcaagt cggccctcga agccacccag accggctacg aggtaggcac
     4801 ccgcaacatc gtcgacgtgc tggatgcgca gaaccgactc tacgcatccg agttcgacta
     4861 cgccgactcc cgctacaact acatcatcga tctcatgcag ctcaggcaaa cggccggcac
     4921 cctcaacggg gaggacgtcg agcagctgaa cgtgtacgcg gactccaatg accccgtgga
     4981 gcggctcaac tcgctgaaag cccgagtcat caactgagcg gaccgcctcc gctaacgcgg
     5041 ccgacgtgct cgcgcagaag cgcgagcaac cgctcggtcg ctccggtatt cgcggcgacg
     5101 acccggcgcg cggacgcggc catcctctcc cgcaactcgg catcgcccag gcagcgcgcg
     5161 acctcagcgg caaggccggc cgcgtcgccg acccgcaaga gagctgccga gtcctcgaac
     5221 gcggccacca cgtcagggaa attgaacgtg tgggggcccg tcagcagagg ctgaccgcac
     5281 agggccggct caatggggtt gtgtcccccg acaggcacca ggctgccgcc gatgaaagcg
     5341 gcctgggaca aaccgtacag gtagagcagc tgccccatga cgtcgccgat caccacgtcc
     5401 accgtcaccc ctgcttccgc gccggcagcg acgctctgcc tgaccacgga aaatcccgcc
     5461 tcctgaacca tcttctccag gtccgccgcc cagctcgggt gccgcggcac cagcagcagg
     5521 cacgcaccag gacaagcgct gcgaaccgca tcgtgcgc
//
LOCUS       DBNHOX010000283         6717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1116729, whole genome shotgun sequence.
ACCESSION   DBNHOX010000283 DBNHOX010000000
VERSION     DBNHOX010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1116729"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /gene="sppA"
                     /locus_tag="AC16UZ_06195"
     CDS             complement(<1..1529)
                     /gene="sppA"
                     /locus_tag="AC16UZ_06195"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR6246311.1"
                     /translation="MSEPGLMSRIGSWLTRARNFTANLIFVLLVVLFVTVILTSGERI
                     RVPEGAALVLNPTGIVVDKETLGDAIAELLAPESTRRETELAGLLTALETGAGDDRIK
                     TLVLDLTELDFVSTAHAATLGEAIRTFSDAGKEVIAYAHYFTQPQYLVASYADAVYMH
                     PDGQILPIGYEVNRLFFSEMLNKLDINVHIFRVGKYKEFVEPYTRSDMSDEAREANQK
                     LVDGLWRHYSETVTANREIEAQSFESYAEQYSPLLKAAAGDMGRLALEFHLVDELLTP
                     DQTRARIAATVGYAREGEFNGIGYRDYLTARDSDPARSGGANVGVITTQGPIVMGSDG
                     RGVIAADSTVDLIRRARMDDSVSALVLRVNSPGGSAFASELIRQELELTQLAGKPVVV
                     SMSSVAASGGYWIASTANRIFAHPTTITGSIGIFAVIPTFEESLARIGVTSDGVGSSA
                     LSGSLDLSSGIAEPMAAVLQASVERGYEQFLNLVARGRDMTPEAVDEIAQGRVWIGST
                     AL"
     gene            1637..3262
                     /locus_tag="AC16UZ_06200"
     CDS             1637..3262
                     /locus_tag="AC16UZ_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6246312.1"
                     /translation="MANLYENDLDANAANFAALTPLTFLARARDIYPHRTAVIYGERR
                     YTWSEHYERCVRVSDALRRRGVGSGDTVAILAGNIPEMLEAHFAVPMAGAVLNAINVR
                     LDAPTVRFILEHGQAAVLLVDEAFGELASHALEGMATAPLVVGIEDSSLEDGRLIGAA
                     TWNELVAEGSSEATWALPGDEWAAIALNYTSGTTGNPKGVVYHHRGAYLNAVSNLLTW
                     NMGDHPTYLWTLPMFHCNGWCFPWTLAVNAGTSVCVRQVRADEIFDAIARYGVSHFCG
                     APVVLNTLLSADDGLKAKVTHQVNVMTAGAAPPAAVIEGMEAMGMSVTHVYGLTETYG
                     PVTLCAWQGERWDDLSPSERAALKARQGVRGHMLEGLMVADPETLVPVPMDGATLGEV
                     MMRGNNVMKGYLKNPDATAQSFEGGWFHSGDLAVWHPDGYIQIKDRSKDIIISGGENI
                     SSIEIEDTLYRHPKVREAAVVAKSDARWGETPCAFVELKPDVAASAEEIIDFCRENMA
                     RFKAPKHVVFGELPKTSTGKVQKFVLRERAEQI"
     gene            3264..4646
                     /locus_tag="AC16UZ_06205"
     CDS             3264..4646
                     /locus_tag="AC16UZ_06205"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR6246313.1"
                     /translation="MSVDASPPLWTWPELCRALSLEVCEGPGVSGIVIDSRKAGPGDL
                     FIALTGDPGPRFNPSSRSERDGHDFIDAAVAAGAVGVLAHDGAARDVPQLQVADTLDG
                     LWALGAAGRARLRCPVVAITGSSGKTTTKGMLAAALGAFSTEGSLNNHLGVPLSLALA
                     PANASAAVFEIGTNHPGEIAPLSALVRPHVAVVLNVHPAHAENFRDLDELRIEKLSIY
                     SGLKTGSHLIVEDTVSVHDLPDHLSLVRFGRGDGSDVQLLEVHGQTADYLIGGEPFTA
                     HVPGGGEHRAASLAAVLATLKVLDRPVRDGLQLGDDLVPKGRGNETLINGVTVVDDSY
                     NANPESMKAALRTLAAVPGRKVAVLGDMLELGAEGPAHHRGLAEACSGIDRVYCVGEA
                     MRELFDALPEGQRGSWLPDAGGEGLIDEILEDLRSGDRVLFKGSNRIFWAKGLVNRFS
                     ERLHTFTMSD"
     gene            4712..4900
                     /locus_tag="AC16UZ_06210"
     CDS             4712..4900
                     /locus_tag="AC16UZ_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246314.1"
                     /translation="MANTHLNGRTFNYEGHCYLIVEDNDWEGSELKVKRLDGTRAQTS
                     LPTENVMRCVFPQKPDDR"
     gene            5170..6135
                     /locus_tag="AC16UZ_06215"
     CDS             5170..6135
                     /locus_tag="AC16UZ_06215"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010208395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990140 - molybdopterin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061798 - GTP 3',8'-cyclase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR6246315.1"
                     /translation="MIVDALGRRFRNLRVSLTAACNYACSYCVPDGKRLQSAQHELDG
                     EELAYAVELVVDAAGIEQLRITGGEPLLSPKFDEVLPRLMALPLNDVSITTNGQLVPR
                     KIDLLLESGLKRINVSLDTLDPLRFRDIARGGDLETVLGGIGALAEGGLKVKINMVPM
                     RTANLDQLVPMLEFCLERGFELRYIELMNMGHLRQGNQYLRDFVGMEEILETIGKRYE
                     FTRTDAPYDSTAVRYEIPDRGFFGIIANETEPFCSSCTRLRLSSNGFIYGCLSNTARH
                     DVKPLLALPRHQAIAQLQGVLVEALADKQDLGFTGEVTVMKFIGG"
     gene            6193..>6717
                     /locus_tag="AC16UZ_06220"
     CDS             6193..>6717
                     /locus_tag="AC16UZ_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HIR6246316.1"
                     /translation="MTDTSSIFEAIVSAQRDRRLPPVASWNPARVGHIDIRIASDGTW
                     YHEGEPIRRKNMVDLFSTVLRRDSDGYCLVTPAEKLLITVEDAPFVAVDFEARGESGG
                     QDLVFVINTGDAVVADSAHPVRVEDHGGVPRPYVMVRDGLEALIARPVFYRLVELAVE
                     EAGELCLYSRGERFV"
BASE COUNT         1198 a         2087 c         2215 g         1217 t
ORIGIN      
        1 agcgccgtgc tgccgatcca tacccgtccc tgggcaattt catccaccgc ctccggcgtc
       61 atgtctctgc ctctggccac cagattgaga aactgctcgt atccgcgctc cacgctcgcc
      121 tggagcacgg cggccatggg ttctgcgatg ccgctcgata ggtcgagcga gcccgacaga
      181 gccgagctgc ccacgccgtc ggacgtcacg ccgatgcgcg ccaggctttc ctcgaaggtt
      241 gggatcacgg cgaatatgcc gatggatccg gtaatggtgg tggggtgagc gaagatgcgg
      301 ttggcggtgg aggcgatcca gtagccgccg gacgccgcca cgctgctcat ggagaccacc
      361 accggcttgc cggcaagctg ggtaagctcg agctcctggc ggatgagctc tgaggcgaag
      421 gccgatccgc cgggcgagtt cacgcggaga accagcgcag acacgctgtc gtccatcctg
      481 gcccgacgaa tcaggtccac ggtgctgtcg gcggcgatga cgccgcggcc gtccgacccc
      541 atcacgatgg ggccctgggt cgtgatcacg cctacgttgg ccccgccgga tctggcgggg
      601 tcgctgtccc gggccgtcag atagtcccga tacccgatgc cgttgaactc gccttcgcgg
      661 gcatagccga cggtggccgc gatacgggca cgggtctgat ccggagtgag gagctcatcg
      721 acgagatgga actccagcgc cagtcgtccc atgtcaccgg cagccgcctt cagcaggggg
      781 ctgtattgct cagcgtagct ctcgaagctc tgagcctcga tctcccggtt ggcggtcacc
      841 gtttccgagt agtggcgcca caaaccgtcc accagtttct ggttggcttc gcgggcttcg
      901 tcggacatgt cgctgcgggt atacggctcc acgaactctt tatactttcc aacgcggaat
      961 atatgaacgt ttatatcaag tttgttcagc atctcactga aaaacaatcg gtttacttca
     1021 tagccaattg ggagtatctg accatcggga tgcatgtaaa ccgcatcggc gtagctggcg
     1081 accaggtact ggggctgggt gaagtagtgc gcgtaggcga tcacttcctt gccagcgtcg
     1141 ctgaacgtcc tgatcgcctc acccagggtc gcggcgtggg ccgtggagac gaagtcgagc
     1201 tcggtgagat ccagcaccag ggttttgatc cggtcgtccc cggcgcctgt ttccagggcg
     1261 gtcagcaggc cggcgagctc cgtttcccgg cgcgtcgatt cgggtgcgag cagctcggca
     1321 atcgcatcgc ccagcgtctc cttgtccacg acgatgccgg tgggattgag caccagagcg
     1381 gccccttccg gtacgcggat gcgctctccg gaggtgagga tcaccgtcac aaagagaacg
     1441 acgaggagca cgaatatcag gttggcagtg aagttgcgcg cgcgggtcaa ccagctgccg
     1501 atgcgggaca tcaggcccgg ttcgctcatg gggtggctcc ggattgctta gatctttcaa
     1561 gcatctcatg ctggacgtgc ttgagctagg cggtgccctg tgctgaagtg agaatttcct
     1621 gccggcgccc acgcacatgg ccaatctcta cgaaaacgat ctggatgcga atgcggccaa
     1681 ctttgcggcg ctcaccccgc tgacgttcct ggcacgggca cgcgatatct atccccaccg
     1741 gacggccgtc atctacggcg agcggcgtta cacctggtcc gagcactacg agcgctgcgt
     1801 gcgggtgtca gacgccctga gacggcgtgg cgtgggctcc ggcgatacgg tcgcgatcct
     1861 ggcgggcaac attcccgaga tgctggaggc gcacttcgcg gttcccatgg cgggcgcggt
     1921 gctcaatgcc atcaacgtca ggctcgatgc gccgacggtg cgcttcatcc tcgagcacgg
     1981 ccaggccgcc gtgctgctgg tggacgaggc gttcggcgag ctggcttccc acgcgctgga
     2041 gggcatggcg accgcgcccc tggtggtggg cattgaagac tcgagcctcg aggacggccg
     2101 gctcattggt gcagcgacgt ggaacgagct cgtggccgag ggttcgtctg aggcgacctg
     2161 ggcgctgcct ggtgacgagt gggcggccat cgccctcaac tacacatccg gcaccacggg
     2221 caatcccaaa ggggtggtgt atcaccaccg cggcgcatac ctgaacgccg tttccaattt
     2281 gctcacgtgg aacatggggg accatccgac ctatctgtgg acgctgccga tgtttcattg
     2341 caacggctgg tgtttcccct ggaccctggc ggtcaatgcc ggcacctcgg tctgcgtgag
     2401 acaggtacgc gcggatgaga tattcgacgc cattgcccgc tacggggtct cgcacttctg
     2461 cggcgcgccg gtggtgctga acacgttgtt gagcgccgat gacgggctca aagcgaaggt
     2521 cacgcaccag gtcaatgtca tgaccgccgg ggcggcgcct ccggccgcgg taatcgaagg
     2581 catggaggcc atgggcatgt cggtgaccca cgtgtacggc ctcaccgaga cctacgggcc
     2641 cgtcaccctt tgtgcctggc agggcgagcg ctgggacgac ctgtccccat ccgagcgggc
     2701 ggccctgaaa gcccggcagg gcgtgcgcgg gcacatgctg gaagggctga tggtggcaga
     2761 tccggaaacc ctggtgccgg tgcctatgga cggcgcgacc ctgggcgagg tgatgatgcg
     2821 gggcaacaac gtcatgaagg gttacctgaa gaaccccgat gccaccgctc aatccttcga
     2881 gggaggttgg tttcactccg gtgacctggc cgtctggcac ccggacggct acatccagat
     2941 caaagaccgc tcgaaggaca tcatcatctc cgggggcgag aacatctcca gcatcgagat
     3001 tgaggacacg ctttaccgcc acccgaaggt gcgcgaggcg gcggtggtgg caaaaagtga
     3061 cgccaggtgg ggcgagacgc cgtgcgcgtt cgtggagctc aagcccgatg tggcagcaag
     3121 cgccgaggag atcatcgatt tctgccggga gaacatggcg cggttcaagg cccccaagca
     3181 cgtggtgttt ggcgagctgc ccaagacgtc cacgggcaag gtccagaaat tcgtgctgcg
     3241 ggaacgggcg gagcagatct gaggtgagcg tggacgcatc gcccccgctt tggacctggc
     3301 cggagctgtg cagggccctg agcctggagg tttgcgaagg tcccggcgtg tcgggcatcg
     3361 tgatcgattc gcgcaaggcg ggtccgggag atcttttcat cgcgctcacc ggggatcccg
     3421 gacctcgatt caatcccagc agccgctcgg agcgtgatgg ccacgacttc atcgatgcgg
     3481 ccgtcgccgc cggcgcggtg ggcgtcctgg cccatgacgg cgcagcgcgc gatgtgccgc
     3541 aactgcaggt ggcggacacg ctggacggct tgtgggctct gggtgcggct ggacgagcgc
     3601 gcctgagatg ccccgtggtc gccatcaccg gcagcagcgg caagaccact accaagggca
     3661 tgctggcggc cgctctggga gcattctcca ccgagggcag tctcaacaat cacctgggcg
     3721 tgccgctgtc tcttgcgttg gctcccgcga atgccagcgc ggccgtgttc gagatcggga
     3781 cgaaccatcc cggagagatc gcgccgcttt ctgctctggt gcggcctcac gttgccgtcg
     3841 tgctcaacgt tcacccggcc cacgcggaaa atttccggga tctggacgag ctgaggattg
     3901 aaaagctgag catttatagt gggttaaaga cagggtctca cttgatagtt gaagataccg
     3961 tctccgttca cgacctgccc gaccatctct cgctggtgcg ttttggccgt ggagacgggt
     4021 ctgacgtgca gctgctcgag gtgcacggac agacggccga ctacctgatc ggaggcgagc
     4081 ccttcaccgc tcacgtgccc ggcggtggcg agcatcgcgc ggcgtccctt gccgccgtgc
     4141 tggccactct gaaagttctg gatcgcccgg tgcgcgatgg cctgcagctg ggtgacgacc
     4201 tggtgccgaa gggccgtggc aacgaaacgc tcatcaacgg cgtgacggtg gtcgacgaca
     4261 gctacaacgc caaccccgag agcatgaagg cggctctgcg gacgcttgcc gccgtacccg
     4321 gtcgtaaggt cgccgtgctt ggcgacatgc tcgagttggg tgccgagggg ccggcccatc
     4381 accgggggct cgctgaagcc tgcagcggaa ttgatcgcgt ctactgcgtg ggagaggcca
     4441 tgcgcgagct cttcgatgcg ctgcccgagg gtcagcgtgg ctcgtggctc cccgacgccg
     4501 gcggtgaagg gctcatcgat gagatcctcg aggacctcag atccggcgac cgggtactct
     4561 tcaagggctc caatcgcatt ttctgggcta aggggctcgt caatcgtttc agcgaacggc
     4621 tgcacacgtt tacaatgtcg gattaaacct cacacaactg gccggaactg ctttgccatc
     4681 atgggcgcag cagtaaccaa ggggatcgac gatggcgaat acgcatctca acggccggac
     4741 tttcaattac gagggccatt gttacctcat cgtcgaggac aacgactggg aaggctctga
     4801 gctgaaggtc aaacgcctgg acggcacccg cgcgcaaacc agcctgccga cggagaacgt
     4861 catgcgctgc gtattccccc agaagccgga cgatcgttga aggcaaaaaa aacccggcgc
     4921 ctggggagga cgccgggtac gcaccataaa ggtccgtagg tcacacatac tggaacggca
     4981 aggccgttcc acagccgctg ccctggtcgt tgggggggga gacatggcag cagccgggaa
     5041 caagtaaacc aatcggctgc tcagatttcc ggaactggtt ctcatttacg cgcgcccgct
     5101 agcacatttg cctgagcatg gcgccgtgtc atactccgcc ccaatccacg gggtccgcgg
     5161 ggccagcaga tgatcgtaga cgcgctgggg cgtcgcttcc gcaatttgag ggtcagcctc
     5221 accgccgcat gcaactacgc ctgcagctac tgcgttccgg acggaaaacg cctgcagtcg
     5281 gcccagcacg agctggacgg cgaggagctc gcgtacgcgg tcgagctcgt ggtcgatgcg
     5341 gccggcatcg agcagctgcg catcaccggc ggagaaccgc tgctctcgcc caagttcgac
     5401 gaggtgctgc cccggctcat ggctctgccg ctgaacgacg tctcgatcac caccaacggc
     5461 cagctcgtgc ctcgcaagat cgacctgctg ctcgaatcgg gcctcaagcg gatcaacgtc
     5521 agcctcgaca cgctcgaccc tctccggttt cgggacattg cccggggcgg cgacctcgag
     5581 accgtgctcg gcggcatcgg agcgcttgcc gagggtgggc tcaaggtcaa gatcaacatg
     5641 gtcccaatgc gcacggccaa tctcgatcag ttggtgccga tgctggagtt ctgtttggag
     5701 cgtgggttcg agctgcgcta catcgagctc atgaacatgg gtcatcttcg ccagggcaac
     5761 cagtacctgc gcgatttcgt ggggatggag gagatactcg agaccattgg caagcgctac
     5821 gagttcaccc gaaccgacgc cccttacgac tccacggccg tgcgctacga gatacccgat
     5881 cggggtttct tcgggatcat cgccaacgag accgagccgt tctgcagctc ctgtacccgc
     5941 ctgcggctgt cgtccaacgg cttcatctac gggtgcctct ccaacaccgc ccggcacgac
     6001 gtgaagcccc tgctggcgct gccccgtcat caggccatcg cgcagctgca gggcgtgctc
     6061 gtggaggcat tggccgacaa gcaggacctc ggcttcaccg gtgaggtcac cgtgatgaaa
     6121 ttcatcggcg ggtgagcggg taagaccgat ctgccggtcg gcgccccatc aaagcaagcc
     6181 gttctttgcg acatgacgga caccagcagc atcttcgaag ccatcgtctc ggcgcagcgg
     6241 gaccgccggc tgccgccggt ggcgagctgg aatcctgccc gggtgggcca catcgacatc
     6301 cgaattgcct ccgacgggac ctggtatcac gaaggcgagc ccatccggcg caagaacatg
     6361 gtggatcttt tcagcaccgt gctgcgccgg gactccgacg gctattgcct ggtgaccccg
     6421 gcggaaaagc tcctgatcac ggtcgaggac gcgccgttcg tggccgtgga tttcgaagct
     6481 cgaggggaga gcggcgggca ggacctggtc ttcgtcatca atacgggcga tgccgtcgtt
     6541 gccgacagcg cacatcccgt gcgcgtggag gaccacgggg gcgtgccaag gccctacgtg
     6601 atggtgcgtg acgggctcga agcgctcatt gcccggccgg tcttctaccg gctggtggag
     6661 cttgccgtgg aggaagccgg cgagctctgt ctttacagcc gcggcgagcg gttcgtc
//
LOCUS       DBNHOX010000284         3283 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1694946, whole genome shotgun sequence.
ACCESSION   DBNHOX010000284 DBNHOX010000000
VERSION     DBNHOX010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1694946"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="AC16UZ_06225"
     CDS             <1..339
                     /locus_tag="AC16UZ_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR6246317.1"
                     /translation="NQVQIGHNCHIGAHTVICGCVGIAGSTRIGRHCVLAGKCGIGGG
                     GPVELADGVVLSGGTNVMVSIDRPGVYSGSVLHETHPRWKRNAMRLRRLDDLFRRVAD
                     LEKKVKDQTG"
     gene            400..837
                     /gene="fabZ"
                     /locus_tag="AC16UZ_06230"
     CDS             400..837
                     /gene="fabZ"
                     /locus_tag="AC16UZ_06230"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR6246318.1"
                     /translation="MKDITEIFDYLPHRYPLLLVDRVLEIVPGERIRGFKNVTFNEDF
                     FNGHFPGNPVMPGVLIVEAMAQLSGVLAFETHDRRPSDGYLYYLASIDNTRFKRPVVP
                     GDRLMMESELVADRRGLMKFKLAAFVDDDLACTAEIACMERKL"
     gene            839..1639
                     /gene="lpxA"
                     /locus_tag="AC16UZ_06235"
     CDS             839..1639
                     /gene="lpxA"
                     /locus_tag="AC16UZ_06235"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIR6246319.1"
                     /translation="MIDPRAIIDPSAVIGEGVTIGPWTFIGPDVVIGDGCWVAPHVII
                     RGPTRIGRNNKIYQFATVGEDTPAMAYHGEPTTLEIGDDNVIREGVTIHRGMMQDLGR
                     TVIGSHCLLMAYVHVGHDCIVGDHVIMANNSSISGHVRVGDHANFGGYSGVPQFRRIG
                     AYSHISGMSLVLKDVPAYMTVMGNPASAIGLNIEGMRRRGYSKELIDALRSAHRVVYR
                     EGRTIQDACRRLEPAARDWPEVELFLESIRDSRWGIVRPRGRGGETRN"
     gene            1636..2853
                     /gene="lpxB"
                     /locus_tag="AC16UZ_06240"
     CDS             1636..2853
                     /gene="lpxB"
                     /locus_tag="AC16UZ_06240"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIR6246320.1"
                     /translation="MSWSAGAADTDPAAPRGDICLGILAGEASGDSLGAGLMAALKRR
                     YPRARFVGIGGPRMLAQDLDPVAPMERLQVNGLVDPVKRLPDLVRLLRQLVRCYEASR
                     PAVFIGVDFNVFNFLLERMLKARGIPTVHYVSPSVYAWRRGRVGRIARSADLLLTLFP
                     FEPALYDDAGLEAVFVGHPRADAIDIDAGGPEGRARARRELQIPPDRFCMAVLPGSRL
                     SEVRLMGAPFLDAAGIVARRLPGTLVIVPCLRPEIRTLLEALLAARPELDAVLYDGDA
                     ELALTASDGALVKSGTSTLEAMLLRRPMVVSYKLGPLTYRLIRLLVRTPHIALPNILA
                     GEELVPEVLQDEATPEALAEKFLDQLDKARRDPEYLARFSDLHRQLRMNADERAADAV
                     SRLLDGRAAHTEA"
     gene            2853..>3283
                     /locus_tag="AC16UZ_06245"
     CDS             2853..>3283
                     /locus_tag="AC16UZ_06245"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008566891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR6246321.1"
                     /translation="MGLFDDLAGTNLTAGVDEVGRGPLAGPVVAGAVVLDPLRPIDGL
                     KDSKRLNARRRAELAEEIRAKALAWGLGRAEVEEIDALNILRASHLAMQRAVAALSVE
                     PEMILVDGNLLPVFSVPAVAVVKGDARVPEISAGAILAKVAR"
BASE COUNT          558 a         1028 c         1107 g          590 t
ORIGIN      
        1 aaccaggtgc agatcggcca caactgccac atcggcgccc acaccgtgat ctgcgggtgc
       61 gtggggattg cgggaagcac acgaatcggc agacactgtg tgctcgccgg caaatgcggc
      121 atcggcggag gcgggcctgt ggagctggcc gatggggtag tgctcagtgg cgggaccaac
      181 gtgatggttt ccatcgatcg gcccggtgtg tattcgggct cggtgctgca cgagacccat
      241 ccgcgctgga agcggaacgc catgcggctg cgccggctgg acgatctgtt ccggcgagtg
      301 gcggacctcg agaagaaggt aaaggaccag acaggctgat cggcagtttt ggctttcggg
      361 tcgatcagcg caaacccgac aaagaacgtg gtcatcggca tgaaagacat caccgaaatc
      421 ttcgactatc tcccccatcg ctacccgctg cttctcgtgg accgcgtgct ggaaatcgtc
      481 ccgggggagc ggattcgggg attcaagaac gtgaccttca acgaagactt cttcaacggt
      541 cactttccag gcaaccccgt gatgccgggc gttctcatcg tcgaagccat ggcgcagctc
      601 tcgggcgtgc tggccttcga gacccatgac cggcgccctt cggatggcta cctctactat
      661 ctggcgagca tcgacaacac ccgattcaag cgtccggtgg tgcccggtga ccgcctgatg
      721 atggagagcg agctggtggc agaccggcgg gggctgatga aattcaagct cgcggcgttc
      781 gtggacgacg atcttgcctg cactgcggag atcgcctgca tggaacggaa gctctgaggt
      841 gatcgacccg cgcgccatca tcgacccgag cgcggtcatc ggggaaggcg tcactatcgg
      901 tccgtggacg ttcatcggcc ccgacgtggt gatcggcgac ggctgctggg tcgcgcccca
      961 tgtgatcatc aggggcccaa cccgaatcgg ccgcaacaac aagatttacc agttcgccac
     1021 ggtgggcgag gacacgccgg ccatggcgta tcacggggag cccaccaccc tggagatcgg
     1081 cgacgacaac gtgatccgcg agggcgtcac cattcatcgc ggcatgatgc aggatctcgg
     1141 cagaaccgtg atcggcagcc attgtctgct catggcatac gtgcacgtgg gccatgactg
     1201 catcgtgggc gatcacgtga tcatggcgaa caactcgtcc atctccgggc atgtgagggt
     1261 aggagatcat gccaatttcg gcggttactc gggcgtcccg cagtttcgcc ggatcggcgc
     1321 ctactcccac atttcaggca tgagcctggt gctgaaggac gtgccggcct acatgacggt
     1381 gatgggcaat ccggcctctg ccattggcct gaacatcgaa gggatgcggc ggcgcggtta
     1441 ctccaaggag ctcatcgacg cgcttcgcag cgcgcacagg gtcgtctatc gcgagggcag
     1501 gaccattcag gatgcctgtc gacgcctgga gccggcggcc cgggactggc ccgaggtcga
     1561 gttgttcctg gaatccattc gggactcgcg atggggcatc gtccgtcccc ggggtcgcgg
     1621 cggcgaaacc cgaaattgag ctggtcggcg ggagctgccg acaccgatcc ggcggcgccg
     1681 cgcggcgaca tctgtctggg tattctcgcc ggagaggcat ccggggacag cctcggagcg
     1741 gggctcatgg ccgcgctcaa gagacgctac ccgcgcgctc gtttcgtcgg catcggtggc
     1801 ccgcggatgc tggcgcagga tctagatccg gtcgcgccca tggagcgcct gcaggtcaac
     1861 gggctggttg accccgtaaa gcgccttcct gatctggtcc gccttctgcg gcagctcgtg
     1921 cgttgctacg aggcctcccg tccggctgtg ttcatcggcg tcgacttcaa cgtgttcaac
     1981 tttctgctgg agcggatgct caaggctcga ggcatcccga ccgtgcacta tgtcagcccc
     2041 tcggtttacg cctggcgccg cgggcgcgtt gggcgcatcg cccgatctgc cgatctgctc
     2101 ctcaccctgt ttccgttcga gccggcgttg tatgacgatg ccggtctcga ggcggtgttc
     2161 gtgggccatc cccgggcaga cgccatcgac atcgacgcgg ggggacccga agggcgggcg
     2221 cgggctcgtc gcgaactcca gatccctcct gaccggttct gcatggcggt tctgcccggc
     2281 agccggctct ccgaggtccg gctcatgggc gcgccgtttc tcgacgcggc aggcatcgtc
     2341 gcccggcgtc tgcccggcac cctggtgatc gtgccgtgcc tccgcccgga gatccgcaca
     2401 ttgctggagg cattgctggc cgcccggccg gaactggacg cggtgctcta cgacggcgat
     2461 gccgagctgg cactgacggc ctcggacggc gcgctggtca agtccggcac cagcacgctg
     2521 gaggccatgc tgctgcgccg gccaatggtg gtgagctaca agcttggccc cttgacctat
     2581 cggttgatcc ggctgctggt gagaactccc catattgcgc tgccgaacat ccttgcaggg
     2641 gaggaactgg tgccggaagt gcttcaggat gaggccaccc ccgaggcgct cgcggaaaaa
     2701 ttcctcgatc aactggacaa agccagacgc gaccctgaat atttagcccg gttctcagac
     2761 ctgcaccgac aactcagaat gaacgccgat gaaagggcgg cagatgccgt gagccggctg
     2821 ctggacggcc gcgccgcgca taccgaggct tgatgggact attcgacgat ctcgccggta
     2881 ctaacctcac cgcgggcgta gacgaggtag ggcgtgggcc cctcgccggt ccggtcgtgg
     2941 ctggcgccgt ggtgctcgat ccgctgaggc ccattgacgg cctcaaggac tccaagcggc
     3001 tgaacgccag gcggcgggcc gagctggctg aggaaatccg ggctaaggcc cttgcgtggg
     3061 ggctcggacg cgcggaggtc gaggagatcg atgcgctcaa catactccgg gccagccatc
     3121 ttgcgatgca gcgggcggta gcggctcttt ccgtggagcc ggagatgatc ctcgtcgatg
     3181 gaaacctgct gccggtcttc tcggttcccg ccgtcgcggt ggtcaaaggg gacgcccgcg
     3241 tcccggagat cagcgccgga gcaatcctgg ccaaggtggc gcg
//
LOCUS       DBNHOX010000285         4263 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1566287, whole genome shotgun sequence.
ACCESSION   DBNHOX010000285 DBNHOX010000000
VERSION     DBNHOX010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1566287"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="AC16UZ_06250"
     CDS             <1..720
                     /locus_tag="AC16UZ_06250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6246322.1"
                     /translation="EAIDVIERVLAAREPISHEGTFWRFENRRLQVPPFQAQPKLAIA
                     GLTGTHNFTTCGQRGYAALSVYFTPVLIEANPGVPDLVDHGRALAQAAEEAGRDPDEA
                     RDDWRISREVYVAESRSQALDDIRDGVRASYDYLMRLGLGPLMKRDAAMPDADVTFEW
                     MVDNIPWIIGSPEECVDQIGSLYEAVGGFGTLLINCRDWVDDAQFHRSMELFARQVVP
                     AFREREHQDLRRSLARDALGE"
     gene            complement(805..2130)
                     /locus_tag="AC16UZ_06255"
     CDS             complement(805..2130)
                     /locus_tag="AC16UZ_06255"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6246323.1"
                     /translation="MDLDGPKESLEAKQPPIDNGLDVVHPSRYTSREFMEREWQHLWP
                     RVWLLAGVVSDIPQPGDYFTYDHGHESFVVVRQQDMGIKAFYNVCPHRGNRIALAEFN
                     STDSFTCAFHGWNFATDGKLTEITEENTFDPKLIAHRPGLNEIRCDVAGGLIFINMDP
                     QAPALRDSIGLPPGYLESYEMEKMHVVRHVRSEWRANWKTGIDAFYETYHLPYVHPQT
                     QTVMEDYSQIDLYPNGAHRMIVPFGTKAHAIADDESVDDGLRFMMIDAGMSPEEFPQK
                     ASEVRGAIQAAKRKRAQRLGLDHYDNFTDGQLIDSWTTGLFPNVQLGMHPEGVFIMRF
                     MPHPTDPERFYYDNMILFRHVDDPDYTVPAWMGLPKDTDVSGAVRPEITHVPVDERPD
                     LGEVLDQDIDLVPVVQKGMRSRGFRGPLWSEMEGRLRHYHKELDRYLER"
     gene            complement(2250..2340)
                     /locus_tag="AC16UZ_06260"
     tRNA            complement(2250..2340)
                     /locus_tag="AC16UZ_06260"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2304..2306),aa:Ser,seq:gct)
     gene            complement(2387..2578)
                     /gene="csrA"
                     /locus_tag="AC16UZ_06265"
     CDS             complement(2387..2578)
                     /gene="csrA"
                     /locus_tag="AC16UZ_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HIR6246324.1"
                     /translation="MLILTRREGESLMIGDEISVTILSVKGNQVRLGVKAPKDVVVHR
                     EEVYQRPESRVSQGGRNNQ"
     gene            complement(2704..3936)
                     /locus_tag="AC16UZ_06270"
     CDS             complement(2704..3936)
                     /locus_tag="AC16UZ_06270"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR6246325.1"
                     /translation="MALIVQKYGGTSVGDVERIRAVAERVTKLRREGHDVVVVVSAMS
                     GETNRLEALGRSLAGRPNPREFDVLLASGEQVTVALLAIVLGEMRCPAVSYLGSQVQI
                     RTDNHFGKARIEHIDTHRLRQDLDAGRVPVIAGFQGIDLAGNLTTLGRGGSDTTAVAV
                     AASLGVDECQICTDVDGVYTADPRVVDDARRLDHVTFEEMLELASLGSKVLHPRSVEF
                     AGKYHVPLRVVSTFEDGPGTLISTELKSMEQPIVSGIAHARDEAKITMSGVPDVPGVA
                     SRILGPIGRAPIEVDMIVQNTGADGLTDFTFTVKRSDFEHALELLEPVRDELGARELA
                     GDNKIAKVSVVGVGMRSHAGVAMRMFDALAEENINIQMISTSEIKISIVVAERYLELA
                     VRCIHDEFGLGEPDASAE"
     gene            complement(3940..>4263)
                     /locus_tag="AC16UZ_06275"
     CDS             complement(3940..>4263)
                     /locus_tag="AC16UZ_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HIR6246326.1"
                     /translation="KGVKVIAAQLDGDPKTMLQTLDTLKSKLGSCVVVLGNVSGGKAN
                     LIVGVSKDLTDRITAPSILEVVGTKAGARGGGRPDMARAGGGDPDALPGALGSVADLI
                     AQRLG"
BASE COUNT          773 a         1386 c         1266 g          838 t
ORIGIN      
        1 gaagccatcg acgtcatcga gcgcgtcctg gccgcgcggg aacccatctc ccacgagggg
       61 acgttctggc gatttgagaa ccggcgactg caggtacctc cctttcaggc ccaacccaag
      121 ctggccatcg ccgggttgac gggtacccac aacttcacta cctgcggcca acgcggctac
      181 gcagcgctca gcgtttactt cactccggtc ctcatcgaag ccaatccggg cgtcccggat
      241 ctggtggacc acggcagggc actggcgcaa gcggctgagg aggccgggcg tgatccggac
      301 gaagccaggg atgactggcg catcagccgg gaggtctacg tcgctgagag ccgcagccag
      361 gctctggacg acatccgcga cggagtgcgc gcctcttacg actatctgat gaggctgggg
      421 ctgggcccgc tgatgaagcg ggacgcggcc atgcccgatg cggacgtaac cttcgagtgg
      481 atggtggaca acattccctg gatcatcggc tcgcccgagg agtgcgtaga tcagatcggt
      541 tcgctctacg aggcggtcgg agggttcggc actctgctga tcaactgccg ggactgggtg
      601 gacgatgccc agttccatcg ctccatggag ctgttcgctc ggcaggtcgt tccagctttt
      661 cgagagcgcg agcatcagga tcttcgtcgc tccctcgcgc gggacgccct cggcgagtaa
      721 gcgcatcaga cagttggctg gcggtggctg cggtcggaag cttaaccagc cccccgaccg
      781 caagctggca acggcgggcg cagcctagcg ctccaggtag cggtccagct ccttgtggta
      841 gtgccgcagg cgcccttcca tctccgacca aagcggtccc cggaaccccc gggagcgcat
      901 gcccttttgg actaccggca ctagatcgat atcctggtcg agcacttccc caagatcggg
      961 ccgctcgtcc accggcacgt gtgtgatctc cggtcgcacc gcacccgaca cgtcggtgtc
     1021 ctttggtagc cccatccacg ctggcaccgt gtagtcggga tcgtccacgt ggcgaaacaa
     1081 gatcatattg tcgtaataga agcgctccgg gtcggtcgga tgcggcatga accgcattat
     1141 gaagacacct tcgggatgca tgccaagctg gacattcggg aacaggcccg tcgtccaact
     1201 gtcgatgagc tgtccatcgg taaaattgtc atagtgatca agccccaggc gctgggcgcg
     1261 ctttcgcttc gctgcctgaa tggcgccgcg cacttcgctc gccttctgcg gaaactcttc
     1321 aggcgacata ccggcatcga tcatcatgaa tcgcagcccg tcgtcgacac tctcatcgtc
     1381 ggcgatcgcg tgcgccttgg tgccgaaggg gacgatcatt cggtgagcgc cgttcgggta
     1441 caggtcgatc tggctgtagt cttccatgac cgtttgcgtc tgcgggtgca cgtagggcag
     1501 gtggtaagtc tcgtagaacg cgtcgattcc ggtcttccag tttgcccgcc attcgctgcg
     1561 cacatggcgg accacgtgca ttttttccat ctcatagctt tcgagatagc ccgggggcaa
     1621 gccgatcgag tctctcagcg ccggggcctg cggatccatg ttgatgaaga tcaagccgcc
     1681 ggctacgtca caacggatct cgttgagccc gggccgatga gcaatgagct tgggatcgaa
     1741 cgtgttctcc tctgtgattt cagtgagctt gccgtcagtc gcaaaattcc agccatgaaa
     1801 agcgcacgtg aagctgtcgg tgctgttgaa ctccgcaagc gcaattcgat tgccccggtg
     1861 tgggcagacg ttgtagaagg ccttgatacc catgtcctgc tggcgcacca caacgaacga
     1921 ctcgtgaccg tggtcgtagg tgaagtagtc accgggctgg ggtatgtcgg acaccacgcc
     1981 ggcgagcagc cacacccgcg gccacaggtg ctgccactcg cgctccatga actcccggga
     2041 tgtgtagcgg gatggatgga ccacatccag cccgttgtcg atcggcggct gtttggcttc
     2101 gagcgactct tttggcccat cgagatccat cggccaccgg acgttcaggc gcgagctcat
     2161 gggtttcctc ctcaaccgtg gaatcggacc actatcctag cacggagccg ttgtctccaa
     2221 gacaccggga gcgaccgcgg ttgtagttct ggcggagagg gagggattcg aaccctcgat
     2281 gagctattaa cccatactcc cttagcaggg gagcgccttc agccactcgg ccacctctcc
     2341 gggagggccg aagtttaccg gccgacggcc accgtagcca gcgatgctac tggttgttgc
     2401 gcccgccttg ggacacgcgt gattccggcc gctggtagac ttcttcgcgg tgaaccacca
     2461 cgtctttcgg cgctttgacg ccgagacgga cctgattacc tttgacgctg agaatcgtca
     2521 ccgaaatctc atcgccgatc atcaggcttt caccttcccg tcgggtcagt atcagcatgc
     2581 ctgtgctccg ctgcccggcg tgctgtcctt tccctcgctc gcggcttatc aattgaggct
     2641 gatcgggccc gctccatcga gccgtgctaa ccgtttcgcc ttatcgtttc gcgattcgac
     2701 gccctattcg gcgctcgcgt caggctcgcc cagtccgaac tcatcatgaa tgcagcgaac
     2761 ggcaagctca agataccgct ccgctacgac aatggaaatc tttatctcgg aagtcgatat
     2821 catctggatg tttatgttct cctcggccag agcatcgaac atccgcatgg cgacgccggc
     2881 atgggagcgc atgccgacac ccaccacgga caccttggcg atcttgttgt caccggcaag
     2941 ttcccgcgcg ccgagctcgt cgcgcaccgg ctccagaagc tccagagcgt gctcgaagtc
     3001 ggaccgcttg acggtgaacg tgaagtcggt gaggccatca gcgcccgtgt tctgaacgat
     3061 catgtcgacc tcgatggggg cacggccgat gggcccgaga attctggagg cgacgccggg
     3121 cacgtccggc accccggaca tggtgatctt cgcttcatcc cgagcatgcg cgatccccga
     3181 tacgatgggc tgttccatgc ttttcaactc cgtactaatc agcgttcccg gtccatcttc
     3241 gaaagtggag acaacccgca gaggaacgtg gtacttgccg gcaaactcca ccgagcgcgg
     3301 atgcagaact ttcgagccga ggctcgcaag ctccagcatc tcctcgaaag tcacgtggtc
     3361 gagtcggcgg gcatcgtcga cgaccctggg atcagccgtg tagacaccgt ccacgtcagt
     3421 gcagatctgg cattcgtcca cgcccagcga tgccgccacg gccaccgccg tcgtgtcaga
     3481 cccgccccgg ccgagggtcg tgaggttacc ggcaagatcg atgccctgga atcccgcaat
     3541 cacgggcacc cttccagcgt cgaggtcctg cctgagccga tgcgtatcga tgtgctcgat
     3601 gcgcgcctta ccgaaatgat tgtccgtccg gatctgcacc tggctaccca ggtacgagac
     3661 ggcaggacac ctcatctcac cgagcacgat ggcaagcagc gcaacggtca cctgctcgcc
     3721 tgaagccagc agaacgtcga actcccgagg attcggtcgg cccgctaggc tccggccgag
     3781 ggcttccaga cgattggttt ccccgctcat tgcggacacg acgacgacca cgtcgtggcc
     3841 ctcccgccgc agcttcgtaa cccgctcggc gacggcccgg atgcgctcca cgtcacccac
     3901 actggtgccg ccgtatttct gaacgatcag agccatggtt cagcccagtc gttgcgcgat
     3961 cagatcggca acgctcccaa gcgccccagg cagagcgtct gggtcaccac cgcccgctct
     4021 ggccatatcg ggccgcccgc caccccgggc gccagccttg gtccccacca cctccagaat
     4081 gctcggggcg gtgatgcgat ccgtcagatc cttgctgacc ccgacgatga ggtttgcctt
     4141 gccgccagac acgttgccaa gcaccaccac gcagcttccg agcttggatt tgagggtgtc
     4201 cagggtctgc agcatcgtct tcgggtcacc gtcgagctgc gcggcgatca ctttcactcc
     4261 ctt
//
LOCUS       DBNHOX010000286         4684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1309334, whole genome shotgun sequence.
ACCESSION   DBNHOX010000286 DBNHOX010000000
VERSION     DBNHOX010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1309334"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="AC16UZ_06280"
     CDS             complement(<1..621)
                     /locus_tag="AC16UZ_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HIR6246327.1"
                     /translation="MKTGIVLIAIAAVLGGLVGTLVVRDPGYVLLAYDDMAVETSLWF
                     ALVALVVIYFVVRLLVFAFSRLAATGGSFGAWNRQRQSKKAREQTIRGLLLMAEGEWA
                     EARRLLVLAAPRVDSPLINYLSAARGAHATGDVEGRDELLRKAHQSTPGARFAVGLTQ
                     AQLQASAEQWEQCLASLLQLQSESPRHQLVLSMLAECYEALQDWRAL"
     gene            complement(618..1721)
                     /locus_tag="AC16UZ_06285"
     CDS             complement(618..1721)
                     /locus_tag="AC16UZ_06285"
                     /inference="COORDINATES: protein motif:HMM:NF016275.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR6246328.1"
                     /translation="MDDDTSKGPAGDDAIGEPAPEATESASRTSGEAADQAAKAASAK
                     PRGRLLGGLGLLLALGALCGVGYLYYLLVFLGPSDELDARLGRLEASLPEIRRDLAAA
                     RDAQAEEIESAVTAQQASLDETRRALLGALNEVTAAAAPAPREWKIAEVEYLLRIANH
                     RVLMEGDVEAALQLMGAADVILEELDDFAFFQVRARLADEMLALKGVRGGDLQGVYLR
                     LEAIKTSIGELPVRLPDYFRPESSADASVEAEGFWSALAAELSGYFQLRRFDGATKPL
                     MAPEENAYLELNLRLAMERAQLAVLRRNQLVFERSLSSAREWILELLDPDDPSVEQVA
                     EELDALSTLKLERDLPDVSGSLTALLELRRGNP"
     gene            complement(1721..2422)
                     /locus_tag="AC16UZ_06290"
     CDS             complement(1721..2422)
                     /locus_tag="AC16UZ_06290"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR6246329.1"
                     /translation="MRVWVTRTQPGADRQARRLRAAGHEPVVGPALRIVEVPGGAPRG
                     PFTDVVYLSEHAVRFGIARLTNQAWFSGARAHAVGAATAEHLAVAGIRAGFPEQASSE
                     GLLSLPEFADARGLTVLIVCGEGGRDLLETTLTARGARVEKLVVYRRRAVDEAPPDVG
                     SVDAIAVSSGDGFAAVARLWFAAGGRGDVPVLAPSPRVAGLGSALGFSNVYTCAGAGA
                     DAVLKALESVSSTGR"
     gene            complement(2424..3341)
                     /gene="hemC"
                     /locus_tag="AC16UZ_06295"
     CDS             complement(2424..3341)
                     /gene="hemC"
                     /locus_tag="AC16UZ_06295"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR6246330.1"
                     /translation="MSALRLVIATRQSQLALWQANFVSARLREAHPGLTVELLGITTR
                     GDRWLSSPLAEVGGKGLFIKELEEALASGTADVAVHSVKDVPAELPEGFAMPVIAYRE
                     DPRDVLVSRDGVTLAGLHAGARVGSSSLRRQAQLLARRPDLLIEPIRGNVDTRLRKLD
                     DGDYDALVLAAAGLARLGLAGRASEYLATDDCLPAAGQGALGIECRAGDGRVRALLEP
                     LEDAQVAACVCAERTVSAVLGADCTAPLAAHATPLGQGVMLQSRLATTDGRKILEARG
                     EGADPVAVGKSVAESLLAQGAAEVLGGGD"
     gene            complement(3338..4066)
                     /locus_tag="AC16UZ_06300"
     CDS             complement(3338..4066)
                     /locus_tag="AC16UZ_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003157680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR6246331.1"
                     /translation="MRVLIVDDEQLARDRLSRMVAELDDYEVAGEAGTGQDAVRLVAD
                     LQPEIILLDIRMPGMDGLEAARHLESVDEPPAVIFCTAYEEHAIEAFNLQAVGYLLKP
                     VRRENLRQALGNAQRVNKAQLAALSDDGSRRRSHISARTRKGIELVPLEAVRYFQADQ
                     KYVTVRHGEGELIIDETLRELEREFGELFIRVHRNALVAARHIIALDRLGDGHYQIRL
                     NDVDEHIDISRRHVASVRKFVRNL"
     gene            complement(4152..>4684)
                     /locus_tag="AC16UZ_06305"
     CDS             complement(4152..>4684)
                     /locus_tag="AC16UZ_06305"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6246332.1"
                     /translation="IIASLIATDPETAESVVEDLSELFRASLNDAGNQVPLQDELELC
                     ERYVRIESLRLGDRLRVEWNVDDSGQNLKIPLLTLQPLLENAIYHGVQPLPEGGTITV
                     HLWLEGNDVNVRITNPIPEGEASNSSAGNRMAVNNIRSRLMVLYGTRAELRAAPEEGL
                     FVTSLKYPVSNMPLQT"
BASE COUNT          788 a         1638 c         1522 g          736 t
ORIGIN      
        1 gagagcccgc cagtcctgca gcgcctcgta acactccgca agcatagaca gcaccagctg
       61 atgacgcggc gactcggact gcagctggag aagcgaagcc aggcattgct cccactgctc
      121 agccgaagcc tgcagctgcg cctgggtgag ccccacggcg aatcgcgccc ccggtgtcga
      181 ctggtgggcc ttgcgaagca gctcgtcgcg gccttcgacg tcgcccgtgg cgtgggcgcc
      241 ccgagcggcg ctcaagtagt tgatcagggg cgaatccacc cgtggggcgg ccagaacgag
      301 cagccgccgc gcttcggccc actctccctc ggccatgagc agcagcccgc gaatcgtctg
      361 ttcacgcgcc ttcttcgact gccgctggcg gttccatgcg ccgaagctgc cgcccgtggc
      421 ggccagccgt gagaatgcga aaacaagcag acgcacgacg aaatagatga cgaccagagc
      481 aacaagcgcg aaccagagac tggtctcgac ggccatgtcg tcataagcca gcagcacgta
      541 ccccgggtca cgcaccacca gcgtgccgac cagcccgccc agcacggcgg cgatggcgat
      601 cagcacaatg cccgtcttca cgggttgccc cggcggagct cgagcagcgc ggtgagggag
      661 cccgacacgt cgggcagatc ccgctcgagc ttgagcgtgc tcagcgcgtc cagctcctcg
      721 gcaacctgct cgacgctcgg atcgtcaggg tcgagtaact cgagaatcca ttcgcgggcg
      781 ctggagaggc tccgctcgaa gacgagctga ttgcggcgca gaacggcgag ttgggcacgc
      841 tccatggcca gccgcaggtt gagctctaag taagcgttct cttccggcgc catgagcggc
      901 ttggtcgcgc cgtcgaatcg gcgcagctga aaatagccgg aaagctccgc ggccagcgcg
      961 ctccagaagc cttcagcctc cacagacgcg tctgcagagc tctccggtcg gaagtagtcg
     1021 ggcagccgga cgggcaactc gccgatgctg gtcttaatgg cttccaggcg aaggtaaacg
     1081 ccctgcaggt cgccgccgcg gacgcccttg agcgccagca tctcgtcggc cagccgggcg
     1141 cgaacctgaa agaaggcaaa atcgtcgagc tcctcgagaa tgacatcagc agcccccatg
     1201 agctgcaggg cggcctccac gtcaccctcc atgagcaccc gatggttggc aatcctgagc
     1261 aggtactcca cctcggcgat cttccattcc cggggggcgg gggccgcggc ggcggtgacc
     1321 tcgttgagcg ccccgaggag cgctctacgg gtctcgtcca gtgacgcctg ctgagcggtg
     1381 acggcgctct cgatctcctc ggcctgcgcg tcgcgcgccg ccgcgagatc tcggcggatc
     1441 tctggaagag acgcctccag gcgcccgagc cgggcatcga gctcatccga cggtcccagg
     1501 aagacgagga ggtaatagag atagccgacc ccgcaaagcg cgcccagggc gagcaggagg
     1561 ccaagcccgc cgagaaggcg gccgcgcggt tttgccgacg ctgccttcgc ggcctggtca
     1621 gctgcctccc ctgaggtcct ggaagcggat tctgtcgcct ccggggcggg ctcaccgatg
     1681 gcgtcgtcgc cggccggacc tttgctggta tcgtcgtcca tcacctaccc gtcgaactca
     1741 cggactcgag cgcctttagc acggcatcgg cgccggctcc cgcacatgtg tacacgttac
     1801 tgaaccccaa tgcggaaccc agcccggcca cccgtgggga cggcgccaac accggcacgt
     1861 ccccgcgacc gcctgcggca aaccataacc gcgccaccgc cgcgaatcca tcgccgctgg
     1921 agacggcgat ggcgtccacc gagccgacat ccggaggggc ctcgtccacg gcgcggcgcc
     1981 ggtagacgac gagcttttcc acccgggcgc cccgtgccgt caacgtcgtc tcgagcagat
     2041 cccgccctcc ttctccgcag acgatgagca ccgtcaggcc ccgggcatcg gcaaattccg
     2101 gcagcgagag cagcccctcg gagcttgcct gctcagggaa gcccgcacga atcccggcaa
     2161 cggccaggtg ctcggcggtc gctgctccca cggcatgggc gcgcgcaccg gagaaccacg
     2221 cctggttggt caggcgcgca atgccaaacc gaacggcatg ctcggaaaga tagaccacgt
     2281 cggtaaaagg tccgcgaggc gcgccgccgg gcacctccac gattctcagc gccgggccca
     2341 cgaccggctc gtggccggcc gctctcagcc ttcgggcctg gcggtcggcg ccgggttggg
     2401 ttcgggtcac ccagacgcgc aactcaatct ccgccgccga gcacctcggc cgcgccctgc
     2461 gccaaaagcg actcggcgac cgatttgccc acggctaccg gatcggcccc ctcgccacgg
     2521 gcttccagaa tcttccggcc gtcggtggtc gccagacggg attgcagcat cacgccttgg
     2581 ccgagcggcg tggcgtgtgc ggccagcggg gccgtgcaat ccgctcccag cacggcgctc
     2641 accgtgcgct cggcgcaaac gcaggcagca acctgagcat cttcgagggg ttcgagcagt
     2701 gcccgcacgc gcccgtcgcc cgcccggcac tcgatgccca gcgctccctg gccggcggcg
     2761 ggcaggcagt cgtcggttgc caggtactcg ctggcgcgcc ccgccagtcc cagccgggcg
     2821 agacccgctg cggccagcac cagggcgtcg taatcgccgt cgtccagctt gcgcagccgg
     2881 gtgtccacgt tgccccggat gggctcgatc agcagatcgg ggcgccgggc caggagctga
     2941 gcctgcctgc gcaggctcga tgagccgacc cgggcaccgg cgtgcaaccc ggccagagtc
     3001 accccatccc ggctaaccag cacgtcacgg ggatcctcac gataggcgat gaccggcatg
     3061 gcgaatcctt cgggaagctc cgccggcacg tcctttaccg agtgcacggc gacgtcggcc
     3121 gtgcccgagg caagcgcttc ttcgagctcc ttgatgaaca ggcccttgcc gccgacctcg
     3181 gcgaggggcg aagaaagcca tcggtcccca cgggtggtga tgccgaggag ttccacggtg
     3241 aggcccggat gggcctcgcg aagacgcgcg ctgacgaagt tggcctgcca caaggcaagc
     3301 tgactctgcc gcgtggctat gacgagccgt aatgcgctca caggttcctg acaaacttgc
     3361 gcacggaggc cacgtggcgc cggctgatgt cgatgtgctc gtcgacgtcg ttgaggcgga
     3421 tctggtagtg gccgtcgccc agacgatcga gcgcgatgat gtggcgcgcg gccaccaggg
     3481 cgttgcgatg gacgcggatg aacagctcgc cgaactcgcg ctctagttcg cgaagggttt
     3541 cgtcgatgat cagctcgccc tcgccgtggc gcacggtgac gtacttctga tcggcctgaa
     3601 agtagcgcac ggcttcgagg ggtacgagct cgatgccctt gcgcgtgcgc gcggagatat
     3661 gggagcggcg tcggctgccg tcatccgaga gcgctgccag ctgagctttg ttgacccgct
     3721 gagcgttgcc gagcgcttgg cgcaggttct cccggcgcac gggtttcaga aggtaaccga
     3781 cggcctggag gttgaacgcc tcgatggcgt gctcctcgta ggccgtgcag aagatgacgg
     3841 ccgggggctc gtcgaccgac tccagatgac gcgctgcttc cagcccgtcc atgcccggca
     3901 ttcggatatc gagcaggatg atctccggct gcaggtcagc caccaggcgc acggcgtcct
     3961 gcccggtgcc agcctcaccg gctacctcgt aatcgtccag ctcggccacc attcgcgaca
     4021 ggcgatctcg ggccagctgt tcgtcgtcca cgatgagaac cctgatgctc atatcccctc
     4081 cctgagcgtg ctggtctcca ggctcgccgc cgcgctcgcc gccgcgcttg ccgccgcgct
     4141 cgccgccgcg ctcatgtctg caacggcatg ttcgacaccg ggtacttcag cgacgtgacg
     4201 aacagccctt cctcgggcgc cgccctcaat tccgcacggg taccgtacaa caccatcagc
     4261 ctgctgcgaa tgttgttgac tgccattcgg tttcccgcgc tgctgttgct cgcctcgccc
     4321 tccggtatcg ggttggtgat gcgaacgttg acgtcattcc cctccagcca cagatgaacg
     4381 gtgatcgtgc cgccttccgg cagcggctgg accccgtggt agatggcatt ttccagaagt
     4441 ggctgcagag tcagcagagg aatcttgaga ttctggcccg agtcgtccac gttccattcg
     4501 acgcgcagcc gatcaccgag ccgcagggac tcgatgcgca cgtaacgctc gcaaagctcc
     4561 agctcgtcct gcagcggcac ctgatttccc gcgtcgttga ggctggcacg aaagagctcg
     4621 gagaggtcct ccacgacaga ctccgcggtc tcggggtcgg tggcgatgag gcttgcgatg
     4681 atgt
//
LOCUS       DBNHOX010000287         2522 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_280890, whole genome shotgun sequence.
ACCESSION   DBNHOX010000287 DBNHOX010000000
VERSION     DBNHOX010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_280890"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="AC16UZ_06310"
     CDS             <1..1060
                     /locus_tag="AC16UZ_06310"
                     /inference="COORDINATES: protein motif:HMM:NF018657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HIR6246333.1"
                     /translation="RGSFAVVTKIHHACIDGVSGVDMVEAIHDVEPYVDRSPGPVTPW
                     KGEQEPSAIELMVRAQVHNMTQPYRLAEVLARTVPALGRLGQRATGGDAERASLAPPR
                     TRFNHVVSAHRVIEGRRFDLAEIRAIKDTVDGATVNDVVLAVCGGALRRYLLAKHELP
                     GVPMIAMAPISVRSSDEKGAMGNRISAMTISLGTHIEDPIGRLSAIHEAAVASKGMSN
                     AVGAKLLTDYSQFIPSTTAAMAARLYVQLGLSERVNIPFNCVVTNVPGPQFPLYSAGA
                     KLLAQYGLGPIFDGMGLIFPVFSYNGKLFLSVTSCREMMPDPEFFSECMQAAFDELKA
                     AALVIPEAADASLAGGTP"
     gene            complement(1081..1755)
                     /locus_tag="AC16UZ_06315"
     CDS             complement(1081..1755)
                     /locus_tag="AC16UZ_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246334.1"
                     /translation="MGQTDSTSRSDVIDQLVASYLKIPLKIYWRETRGLPFPDTFIDA
                     RLEFAGMTTSWLRLDSVIWRAGEVRFVPGFPARLYVRSPSVEFVVGQAELDRWQSRFS
                     LPYRLELTESGITVHTELAGFPLAEFETGLTVVDGWFVLEPKRATVLGVPGYVSSLFR
                     GYLPVPPLSGEIRLAGIEHAAGILRVRFDIDDFDEEVTPGMLSRLQKRLFSFNSPLGP
                     TRRDDA"
     gene            1810..>2522
                     /locus_tag="AC16UZ_06320"
     CDS             1810..>2522
                     /locus_tag="AC16UZ_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246335.1"
                     /translation="MSGQTNSVGGEKVPLVIGVTGHRDLLAEELPVIRRRVQDLFAEL
                     QRQFPDLPILVMTPLAEGADRLVAEVARDMGMPVEVLMPMPRELYQADFSGESLVEFD
                     EMLRLGEIVELPLVPGNTEKTVAAGGRPRDLQYAQLGAYLAAHSHVLLAIWDGKPPEA
                     VGGTAHVVQFHQHDVIELLAEGQHRSPIDFAEDESDLVFHIACSRLADGAPREPLQPG
                     QAEWLTRDDVQPRTPQMPP"
BASE COUNT          449 a          777 c          866 g          430 t
ORIGIN      
        1 ccggggaagt ttcgcggtgg tgaccaagat ccaccacgcc tgcatcgatg gcgtgtccgg
       61 cgtggacatg gtggaggcca tccacgacgt cgagccctac gtcgaccggt cgcccggacc
      121 ggtcacgccg tggaagggcg agcaggagcc cagtgccatc gagctcatgg tccgtgccca
      181 ggtccacaac atgacgcagc cctaccgcct tgcggaggtg ctggcccgta cggtccccgc
      241 cctgggcagg ctcggccagc gggcgacggg gggcgacgcg gaacgggctt ccctcgcgcc
      301 gccgcgaacc aggttcaacc acgtggtatc ggctcatcgt gtgatcgagg ggcggcgatt
      361 cgacctggcg gagataaggg ccatcaagga caccgtggat ggggcgaccg tcaacgatgt
      421 ggtgctggcc gtctgcggcg gcgctctgcg gcgctacctg ctggcaaagc acgagctgcc
      481 cggcgtgccc atgatcgcca tggcccccat ttctgttcgc agctccgacg agaaaggcgc
      541 catgggcaac cggatctccg ccatgacgat ctcgctgggc actcacatag aggaccccat
      601 cgggcggctt agcgccattc acgaagcggc ggtggcctcc aaggggatga gcaacgcggt
      661 gggcgcgaag ctgttgaccg actattcgca gttcatcccg tcgacgacgg cagccatggc
      721 ggcccgcctg tacgttcagc tggggttgag cgagcgggtc aacattccgt tcaactgcgt
      781 cgttacaaac gtgccggggc ctcagtttcc cctgtacagc gcgggagcga agctgctggc
      841 gcagtatggg ctgggcccga tattcgacgg catggggctg atatttcccg tgttcagcta
      901 caacgggaag ctctttctgt ccgtcacctc ctgccgggag atgatgcccg atccggagtt
      961 tttctcggaa tgcatgcagg cggctttcga cgagctcaag gcggctgcgc tggtcattcc
     1021 ggaagccgct gacgcctcgc tggccggtgg aacgccctga tgctcgggag ccgttcgcgg
     1081 ctacgcgtcg tccctgcgcg tcggccccag tggcgagttg aaggagaaca gccgtttctg
     1141 gagccgggac agcatgcccg gggtgacctc ctcgtcgaag tcgtcgatgt cgaatcgcac
     1201 gcgcagaatg ccggcggcgt gctcgatgcc ggccagacgt atctcgccgg acaggggcgg
     1261 gaccggcaga tagccgcgga aaagggatga cacgtagccg ggcacaccga gcacggtggc
     1321 tcgtttgggt tccagaacga accacccgtc cacgaccgtg aggcccgtct cgaactcggc
     1381 tagaggaaat ccggccagct ccgtatgcac ggtgatcccg gactccgtga gctcgagccg
     1441 atacggcagc gagaaccgcg actgccagcg gtcgagctct gcctggccca cgacgaactc
     1501 cacgctgggc gaccgcacgt agagccgagc cggaaacccc gggacgaatc gcacttcacc
     1561 ggcacgccag atcacgctgt ccaggcgcag ccaggaagtc gtcatgcccg cgaattcgag
     1621 tcgtgcgtcg atgaaggtgt cggggaaagg gagcccgcgg gtctcgcgcc agtagatctt
     1681 cagcggaatc ttaaggtagg aagcgaccag ctggtcgatt acgtctgaac ggctcgtcga
     1741 atcggtctgg cccatgagcg gagtttaccc aggctttgcc tgggaacaaa tacgggttaa
     1801 gtttccgcca tgtccggcca gaccaacagc gttggtgggg aaaaggtgcc gctcgtcatc
     1861 ggcgtgacgg ggcatcggga tcttctcgct gaggagctgc cggtgatccg gcggcgggtc
     1921 caggatctgt ttgccgagct gcagcggcag ttccccgatc tgccgatcct ggtcatgacg
     1981 cctctagccg agggggcgga ccggctcgtt gccgaggtgg ccagggacat gggcatgccg
     2041 gtcgaggttc tcatgcccat gcccagagag ctttaccagg cggatttctc gggcgaatcg
     2101 ctagtcgagt tcgacgagat gctgaggctg ggagaaattg tggagttgcc cctcgtgccg
     2161 ggcaacactg agaagacggt ggcggccggg gggcggcccc gtgacctgca atatgctcag
     2221 ctgggagcat acctcgcggc tcacagtcat gttctgctgg ccatctggga tggcaagccg
     2281 cccgaagcgg tagggggcac ggcgcacgtg gtgcagtttc atcagcacga cgtcatcgag
     2341 cttctggcgg aaggacagca ccgctcgccc atcgatttcg cggaagacga gagcgatctc
     2401 gtgtttcaca tagcatgctc gcgtctggcc gacggggcgc cgcgagagcc gctgcaaccc
     2461 ggtcaggcgg agtggctcac gcgtgacgac gtgcaaccgc ggacgccgca gatgccgccg
     2521 ag
//
LOCUS       DBNHOX010000288         2247 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1437193, whole genome shotgun sequence.
ACCESSION   DBNHOX010000288 DBNHOX010000000
VERSION     DBNHOX010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1437193"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="AC16UZ_06325"
     CDS             complement(<1..1206)
                     /locus_tag="AC16UZ_06325"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6246336.1"
                     /translation="MSKSALTSALTKVPFARSVMAKSVSLASAGLVAMPVVSVDAATG
                     SSVIEEVIVTSSRREQSVQDIPINITAVSDTMIKELRLDGIAEIARYVPGLTVVDRGP
                     RDEQPDILVRGLNTTGLGPGFISDTVAVYFGEIPLNVDFKPVDMERVEVLIGPQGTLY
                     GQGTLGGAIRYIPKRADTSALSIDARAKAMSNKESDGLSTDIGVTLNIPLVEDVLAAR
                     VNFDRLNDKGFIDYPFVVQESGVSNPEPDFSDPADVAANLREVEDANGEEATSIRANL
                     RFTPTDGIDVNLWYLNQENETEGRQIANRLAIGTGKYESGLRYEEPNDYENELWAIEA
                     TADLRFAEATLIYGETQYDELGQRDQTDLLLNFEYGYEFFPTFSSFTREILEEESDTL
                     EVRLTSQHGG"
     gene            complement(1385..>2247)
                     /locus_tag="AC16UZ_06330"
     CDS             complement(1385..>2247)
                     /locus_tag="AC16UZ_06330"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6246337.1"
                     /translation="KPFFAERDYSLAPATVAPTGVAEPVEGGYRLAGRWSWGTGVMHG
                     DWVIVSAVVPGDDPVPDMRFFALPIEEITVDDVWHIEGMLGTGSNDILIDGRVIPEER
                     AVSLNAMRDGRAPGAEIHDGPLYRTPMMPVLVMAAAMPAVGQARAVVDAFKQSLQDRF
                     MPMWGITQAERPAAQMRVARAELEMRQTEMLMGEVVEDVMARRNEATLADRARWMASL
                     ALAVDQSKQIVASVATASGASAHFSSHPLQRAARDLSTLSCHAVFDLDARLETAGKAM
                     LGLDPGPIMI"
BASE COUNT          352 a          741 c          724 g          430 t
ORIGIN      
        1 gccgccatgc tgggaggtga gccgcacttc cagggtgtcg ctttcctctt ccaaaatctc
       61 ccgggtgaag gaagagaagg tggggaagaa ctcgtagccg tactcgaaat taagcagcag
      121 gtccgtctga tcgcgctgcc cgagctcgtc gtactgggtt tccccgtaga tgagggttgc
      181 ttcggcgaat cgcaggtcgg cggtcgcttc gatggcccac agctcgttct cgtagtcgtt
      241 gggctcctcg tagcgcagtc ccgattcgta cttgccggta ccgatcgcca gacggttggc
      301 gatctgccgt ccctccgtct cgttctcctg gttcaggtac cagaggttca cgtcgatccc
      361 gtcggtgggg gtgaatcgca ggttggcgcg aatggaggtg gcttcctcac cgtttgcgtc
      421 ctccacctca cgcaggttgg ctgcgacgtc cgccgggtcg ctgaagtcgg gctccgggtt
      481 cgacacgccc gattcctgaa cgacgaacgg gtagtcgatg aagcccttgt cgttgagccg
      541 gtcgaagttg acgcgtgccg ccagcacgtc ctccaccagc gggatgttca acgtcacgcc
      601 gatgtcggtg ctcaggccgt cgctctcctt gttggacatc gccttggcgc gggcgtcgat
      661 gctcagcgcg gaggtatccg cccgcttggg tatgtagcga atggcgccgc cgagggtgcc
      721 ctggccgtac agcgtaccct ggggaccgat caggacctcg acccgctcca tgtcgacggg
      781 cttgaagtcg acgttcagcg ggatctcgcc gaagtagacg gccacggtgt cgctgatgaa
      841 tccgggcccc agccccgtgg tgttcaggcc gcgcaccaga atgtcgggct gttcgtcccg
      901 cggcccccgg tcgaccaccg tcagcccggg aacatatctg gcgatctcgg caatgccgtc
      961 taatcgcagc tccttgatca tcgtgtcgct caccgcggtg atgttgatgg gaatgtcctg
     1021 gacgctctgc tcccggcggc tggaggtgac gatcacttcc tcgatgacgg acgagcccgt
     1081 ggccgcatcg accgagacaa ccggcatggc gacgagcccg gccgaagcca gagacaccga
     1141 tttcgccatg accgatcggg cgaacggcac ctttgtcagt gcagatgtca gtgcagactt
     1201 tgacatgttt cccccaagtt ccggccgggt ggccgaggat cttgtttttt gttgtggaca
     1261 gcttgttttt gaccgcgcag cctatgaact ttgcggcgtt cgtacaaccc gaaccgcaat
     1321 gaaattgagc cgtgagcgcc gatccccagg gtccccgccg agcccgccgc cggcctcgga
     1381 cttttcagat catgatcggt ccggggtcaa gaccgagcat ggcctttccg gcagtctcga
     1441 gccttgcgtc cagatcgaag accgcgtggc acgaaagcgt gctcagatcc cgcgcggcgc
     1501 gctgcagcgg gtggctcgag aaatgcgcgc tggcgccgct ggccgtcgca acggaggcaa
     1561 cgatctgctt gctctggtcc accgcgaggg cgaggctcgc catccaccgc gcccggtctg
     1621 ccagggtcgc ctcgttgcgc cgcgccatga cgtcctcgac cacctcgccc atgagcatct
     1681 cggtctggcg catctccagc tccgccctgg caacgcgcat ctgggccgcc ggccgctctg
     1741 cctgggtgat gccccacatg ggcatgaagc gatcctgaag cgactgcttg aacgcatcga
     1801 ccaccgctct cgcctggccc acggccggca tggcggccgc catgacgagc acgggcatca
     1861 tcggcgtccg gtacagcggt ccgtcgtgga tttcggcacc cggcgcacgg ccgtcgcgca
     1921 tggcgttcag ggataccgcc cgctcttccg ggatcacccg gccgtcgatg agaatgtcgt
     1981 tgctgccggt gccgagcatg ccttcgatgt gccagacgtc gtccacggtg atctcctcga
     2041 tgggcagcgc gaagaagcgc atgtcgggca cgggatcgtc gccgggcacc acggcgctca
     2101 cgatgaccca gtcgccgtgc atgacgcccg tcccccacga ccagcgtccg gcaagccggt
     2161 agccgccttc caccggctcg gcgaccccgg tgggcgccac ggtggccggg gccagggagt
     2221 agtcgcgttc ggcaaagaag ggtttct
//
LOCUS       DBNHOX010000289         1706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1695098, whole genome shotgun sequence.
ACCESSION   DBNHOX010000289 DBNHOX010000000
VERSION     DBNHOX010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1695098"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1706)
                     /locus_tag="AC16UZ_06335"
     CDS             complement(<1..>1706)
                     /locus_tag="AC16UZ_06335"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR6246338.1"
                     /translation="IDTGELSLDERIAIAVELSRALQSVHDEGVIHRDVNPGNVLVSE
                     DPMRVHLIDFGLATLAAREYPEVEPLGQVTGTLPYLSPEQTGRVNRVVDYRTDLYSLG
                     ATLYELLAGNPPFTNTDPLELIHAHIASTPRPLTAIGEGIPRWLSEIVQKLLAKQPED
                     RYQSAAAVRDDLIRGQTQANVVPFRLGQTDSPGLLAMPKRLYGRTAQIGIIQEYLERA
                     ARGEVLFLQITGAQGRGKSSLCAELARQAAGTKAMVARTNALAFDFRDTDAVWIELTR
                     QLLRQALADAKAGDLLARIAALNSPNVRSLTELIPELRDLVPAGSEGAGLPGVGIQEV
                     LKALQPQPLCLIVEEVDNVPSESLETLLSMALDSRHLLMTVTRANADSDAFRTPRLAT
                     KAATLELPAFARADVRSLLSDMLSTSEVRVRELASELHAKTDGLPADVLQLIEELHHL
                     GHIFRDANAAEWEWDMAAIRAHYFSNNSADRIRRLISELSEETTHALQVGAAVGEQFS
                     SELAATLLDVEQAELAGRLRPALTKGLLTTATGSKEAPGDQHQLLYRFAHPRVRSLVY
                     AE"
BASE COUNT          254 a          546 c          590 g          316 t
ORIGIN      
        1 ctccgcgtac accagcgacc tcacccttgg atgggcgaac cggtagagca gctggtgctg
       61 atctcccggc gcttccttgc tgcccgtcgc ggtggtgagc aggcctttgg tcagcgccgg
      121 ccgcagccgc ccggcgagct cggcctgctc gacgtcgagc aaggtggcgg ccagctcgct
      181 cgagaattgc tctcccacgg ccgcaccgac ctgcagcgcg tgcgtcgtct cctcggaaag
      241 ctcgctgatc agccggcgta ttcggtctgc ggagttgttg ctgaagtagt gggcgcggat
      301 ggccgccatg tcccactccc actcggccgc attggcgtca cggaagatgt ggccgaggtg
      361 atgcagctcc tcgatcagct gaaggacgtc ggcgggcagg ccgtcggtct tggcgtgcag
      421 ctcgcttgcg agctcgcgaa cccgaacctc gctggtcgag agcatgtccg acagaaggct
      481 tctcacgtcg gcccgcgcaa atgccggcag ctccagcgtg gccgccttgg tcgccagtcg
      541 gggcgtcctg aacgcgtccg aatcggcgtt cgcccgggta accgtcatca gcaagtgccg
      601 cgagtcgagc gccatgctca gcagggtttc caggctttcc gaagggacgt tgtcgacttc
      661 ctccacgatc aggcaaagcg gttggggctg cagcgctttc aggacttcct ggatgcccac
      721 cccgggcagc cccgcgccct cactgcccgc aggcaccaga tctctcagct ccgggatgag
      781 ctccgtcagg ctgcgaacgt tcggcgagtt cagcgccgcg atgcgcgcca gaaggtcacc
      841 ggctttagcg tcggccagcg cctgtcgcag gagctggcgg gtcagctcga tccagaccgc
      901 gtcggtgtcg cgaaagtcga aggcaagcgc attggtccgg gcgaccatgg cttttgtgcc
      961 ggctgcctgc ctggcgagct ccgcgcatag ggaggacttt cctctgcctt gcgccccggt
     1021 gatctgcagg aagagaactt cccctcgtgc ggcgcgctcc agatattcct gaatgatgcc
     1081 gatctgggcg gtgcgaccgt acaggcgttt gggcatggcc agcagcccgg gcgagtcggt
     1141 ctgtccgagc cggaacggta ccacgttggc ctgggtctgc cccctgatca gatcgtcgcg
     1201 gacggctgcg gcgctctggt agcggtcttc cggttgcttg gcgagcagtt tctggacgat
     1261 ttccgacagc cagcgcggaa ttccctcgcc gatggcggtt aggggccgcg gcgtgctcgc
     1321 gatgtgcgcg tggatgagct ccagcgggtc cgtgttggtg aacggggggt tgccggccag
     1381 aagctcgtag agcgtcgcgc ccagcgagta caggtccgtg cggtagtcca ccactcggtt
     1441 cacgcggccg gtctgctcgg gtgaaaggta gggcagggtc ccggtcacct ggccaagggg
     1501 ttccacctcg ggatattccc gggctgccag cgtagccaag ccgaagtcga tgaggtgcac
     1561 gcgcataggg tcttcggaga cgagcacgtt gccggggttg acgtccctgt ggatcacgcc
     1621 ctcgtcgtga acggactgca gcgcgcggct gagctccacg gcgatcgcga tgcgttcgtc
     1681 cagggaaagc tcgccggtgt cgatga
//
LOCUS       DBNHOX010000290         3100 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1630965, whole genome shotgun sequence.
ACCESSION   DBNHOX010000290 DBNHOX010000000
VERSION     DBNHOX010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1630965"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="AC16UZ_06340"
     CDS             complement(<1..342)
                     /locus_tag="AC16UZ_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246339.1"
                     /translation="MRELTRNNRQSLLSDFADAFERARNFRIAREDQALENYKLYMRK
                     EATAEAARAERNEPPMSGSKLRSPLVYWGVETLLPRLGVQPPTVTATAATPEALPYAA
                     AKQYRLQYYVRM"
     gene            complement(368..685)
                     /locus_tag="AC16UZ_06345"
     CDS             complement(368..685)
                     /locus_tag="AC16UZ_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246340.1"
                     /translation="MAEQGVGAGADPAAAVDNPLGTPAEPSPAETAAMLGMQIGQQAI
                     EAHRMVDQELAQVAEQFNAAHQQIDMGAQQAIQQMQAAIVAGAQAGSGVASGPQAVGA
                     DAV"
     gene            complement(749..2290)
                     /locus_tag="AC16UZ_06350"
     CDS             complement(749..2290)
                     /locus_tag="AC16UZ_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HIR6246341.1"
                     /translation="MGADPAKLQRAAVLIAQHPANLAAFTKLPGERGPIDYRLWPDQR
                     IAVESALTYHQSIILKARQLGMTWTLGALLGLWDALRPIDGETLVLSQTEDDARAVIQ
                     RARYLYGHLPKELREMVFPLKVDRADRLEVDHGDVVSSLISLSSSSAAGRGRTFRRVI
                     CDEQAHWEHADERMAAIMPTVADTGQLVVVSTAKGHNQFRQRYLGAVDPQGQSADEAE
                     ALGNGFVRHFFGALSHPNRDHEWVARKRTQLGDLGPQEYPLTAGEAFVASGACVFDTN
                     ALAIYLDEVCEQPGHRRKAYQRETLLEEAGGQWQIWQTPQEGRTYVITADPCGGGAGR
                     DSAAAVVCDDRSWDQVAAFHGKVEPLELARQMMAAGTYYNTALLAPESNTHGAGVVAT
                     LIDNHYPNLYRRTPTDRFGAGLTAHVGFETTAKSRAEALGFLKDCVRYRDLGIVDAAA
                     VDEMMEFVTLPSGREQAEEGAHDDRVMAWAIMAFVLSNSRLTTGTEYEPMKPYVPRVS
                     SRTGY"
     gene            complement(2280..2771)
                     /locus_tag="AC16UZ_06355"
     CDS             complement(2280..2771)
                     /locus_tag="AC16UZ_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246342.1"
                     /translation="MEAISDVPATTPEERHDVAMSGLGWPQERVAEMKAWIIRATAIG
                     YSLARCRRALQLGDPKMIRKWLTADPAFADAYADAEMELRENMQDEAYRRAFQGYEAG
                     DGKIKYSDRMMELALKAEMPEKFSREATGGGRIVINIEQVERAEIVEGEVEEVVAELT
                     DGG"
BASE COUNT          491 a         1012 c         1021 g          576 t
ORIGIN      
        1 catccgaacg tagtactgca gccggtactg cttggcggcg gcgtagggca gggcttccgg
       61 ggtcgccgcg gtggccgtga ccgtcggggg ctgcactccg aggcggggca gcagggtctc
      121 gactccccag tacacgaggg gcgagcgcag cttggagccg gacatcggcg gctcgttgcg
      181 ctctgcgcgg gcggcttcgg cggtcgcctc cttgcgcatg tacagcttgt agttctcgag
      241 cgcctgatcc tctcgagcga tgcggaagtt gcgcgcccgc tcgaacgcat cggcgaagtc
      301 ggacaggagg ctttggcggt tgttccgcgt cagctcccgc atgtgtggcg cgtcgacgcg
      361 caccagatca gaccgcgtcc gcgccgaccg cctgtggccc ggaggcgact ccagaacccg
      421 cctgtgcgcc agcgacgatc gcagcttgca tctgctgaat ggcctgctgt gcgcccatgt
      481 cgatctgctg atgcgcggcg ttgaactgct ccgcgacctg ggcaagctct tggtcgacca
      541 ttcggtgcgc ctcgatggcc tgttggccga tctgcatacc gagcatggcg gcggtttctg
      601 ccggagaagg ttccgccggt gtcccgagcg gattgtcaac tgcggctgca gggtcggccc
      661 cggctccgac gccctgctcc gccaccggac ccgcctgggg tacgagtggc atctgcgtga
      721 ctcctcgatt gactggcgcg agcatacctc aatacccggt gcgcgacgag acgcgcggca
      781 cgtagggctt catcggctcg tactcggtgc ccgtcgtgag ccggctgttg gacagaacga
      841 aggccatgat cgcccacgcc atcactcggt catcgtgcgc gccctcctcg gcctgctccc
      901 ggccggaagg cagcgtcacg aactccatca tctcgtcgac ggcagcagcg tcgacgatgc
      961 ccaggtcccg atagcgtacg cagtccttca agaagcccag cgcctcagcc cgtgacttgg
     1021 cggtcgtctc gaacccgacg tgagccgtca gccccgcgcc aaagcgatcg gtgggcgtac
     1081 ggcgatagag gttcgggtaa tggttgtcga tcagagtggc gacaactcca gcgccatgag
     1141 tgttcgactc tggcgcaagg agagccgtgt tgtagtacgt gcccgccgcc atcatctgac
     1201 gggccagctc aagcggctct accttgccgt ggaaggcggc gacctggtcc caactgcggt
     1261 catcacagac gacagcggcc gccgaatccc ggcctgcgcc gccgccgcac ggatcggccg
     1321 taatgacata ggtgcggccc tcctgggggg tctgccagat ttgccactgg cctcccgcct
     1381 cctccagcag ggtctcgcgc tgatacgcct ttcgccgatg acctggctgt tcgcacacct
     1441 cgtcgaggta gatggcgagc gcattggtat cgaagacgca ggcaccggag gcgacgaacg
     1501 cctcccccgc ggtcagtggg tactcctgcg ggccgaggtc ccccagctgt gtgcgcttac
     1561 gcgcgaccca ttcgtggtca cggttggggt gcgagagcgc gccaaagaag tgacggacga
     1621 acccgttgcc caacgcttcg gcctcgtcgg cactctgacc ctgtgggtca accgccccga
     1681 ggtagcgttg ccggaactgg ttgtggcctt tcgccgtgga gacgacgaca agctgccccg
     1741 tgtcagcgac cgttggcatt atggcggcca tacgctcgtc tgcgtgctcc cagtgggcct
     1801 gctcgtcaca aatcacccgg cgaaacgtgc gaccccggcc cgcggcggac gacgacgaca
     1861 gcgatatgag cgaggagact acgtccccgt ggtcgacctc gaggcggtcg gcgcggtcaa
     1921 ccttcaacgg gaagaccatc tcgcggagtt ccttgggtag gtgtccgtac aggtagcggg
     1981 cgcgctggat gacggctcga gcgtcgtcct ctgtctgcga gagcaccagc gtctccccgt
     2041 cgatcggccg gagtgcgtcc cacaatccga gcagcgcccc cagcgtccag gtcatcccga
     2101 gctggcgggc cttcaagatg atcgactggt ggtacgtgag ggcggattcg acggcgatcc
     2161 gctggtcggg ccagaggcgg tagtcgatcg gcccccgttc cccggggagc ttggtgaacg
     2221 ccgccaggtt ggcggggtgc tgcgcgatca gaacggccgc gcgctggagc tttgccgggt
     2281 cagcccccat ccgtcagttc ggccacgacc tcttcgacct caccctcgac gatctcggcg
     2341 cgttcgacct gctcgatgtt gatgacgatg cgcccgcccc cggtggcctc gcgggagaac
     2401 ttctcgggca tctcagcttt cagagccagc tccatcatcc ggtcggagta cttgatcttc
     2461 ccgtcgccgg cctcgtagcc ctggaacgcg cggcgatacg cctcgtcctg catgttctcg
     2521 cgcagctcca tctccgcgtc ggcgtaggcg tccgcgaatg ccgggtcggc ggtcagccat
     2581 ttccgaatca tcttcgggtc gcccagctgg agagcgcgcc ggcatcgagc gagcgagtag
     2641 ccgatcgccg tggcgcgaat gatccacgcc ttcatctcag cgacacgctc ctgcggccag
     2701 ccgagcccgc tcatggcgac gtcgtgtcgt tcctcggggg tcgtcgccgg cacgtcagag
     2761 atggcttcca tctcttgccg aatctggttt gtcggatcga cgatggcgat ggccgcgagg
     2821 gtagcggata agggcctgcc cgataaagag cgcgagcgaa gcgagcgcgc tgtcaaccct
     2881 catatggggg tgtcgtacct gtgtactggg aaccgtgtac ccgtgtacct gtgtaccgag
     2941 tacaccccct tcacaaggta taggaaaggg tatctactaa gtattagtaa gttttagttc
     3001 tctgtatgta cgcgcagtag cgcacttggg tacacaggtc cacaggtaca caggtgggcg
     3061 tcttgagtac cgatatggag gcttcggtac tcaggtcagg
//
LOCUS       DBNHOX010000291         5031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_603209, whole genome shotgun sequence.
ACCESSION   DBNHOX010000291 DBNHOX010000000
VERSION     DBNHOX010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_603209"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..692)
                     /locus_tag="AC16UZ_06360"
     CDS             complement(249..692)
                     /locus_tag="AC16UZ_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009404568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HIR6246343.1"
                     /translation="MTRHFSLYELTASEVAERQGIVNVPPPSVVPNMERLMRTLEDVR
                     TAVDNRPVIITSGYRSERLNRHIGGSRTSAHMDGRAADLKVVGLTPHEVAIKIAEAGV
                     VLDQLIYEGSWVHVGIARTHETPRQQLLTARFVQGRPSYQQGILL"
     gene            complement(689..1036)
                     /locus_tag="AC16UZ_06365"
     CDS             complement(689..1036)
                     /locus_tag="AC16UZ_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246344.1"
                     /translation="MNSIEVTLLMVGLVSTSLVILWQNLTAVNLMGPQTHNGVRWAYV
                     LKSAGHFGMILAVFDYLHGDPRAWPWLLLGSVVTTNTGRVLLQIVTRRECHCPECPVR
                     RNRVACQRGRAPE"
     gene            complement(1033..1362)
                     /locus_tag="AC16UZ_06370"
     CDS             complement(1033..1362)
                     /locus_tag="AC16UZ_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246345.1"
                     /translation="MDAPWHKAIPPAMGFFGAVLMMSYLGAMPVRMWLIALASGPVMA
                     YLATDPAVSYLIATFDWIPRTESALVKLGGLVGCIIGLLSIHLVGAAASTGRRFSETP
                     YDPRDRK"
     gene            complement(1377..2003)
                     /locus_tag="AC16UZ_06375"
     CDS             complement(1377..2003)
                     /locus_tag="AC16UZ_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012829.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPRY domain-containing protein"
                     /protein_id="HIR6246346.1"
                     /translation="MIPGIVAAQLAPPAGPGVTYATFDPANKHAGILLSNGDRTATKT
                     TDEALRSVRSTLGVATGKHYWEVTMVTGSVSPFMLLGVGTSAVDLASFVGNSASGWGY
                     YEDTGQKYTNNVGATFGAPWATNGDIIGIALDMDSGKVWFSKNGVWQASGDPAAGTNP
                     AFSGLTGTIYAFASLYRGTPPSHQVTANFGAAAFAHAVPVGFRAGLYT"
     gene            complement(2003..3382)
                     /locus_tag="AC16UZ_06380"
     CDS             complement(2003..3382)
                     /locus_tag="AC16UZ_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246347.1"
                     /translation="MAIVLRSVKGSELTHAELDGNFTELAARALPVFDLATYATVSGS
                     YYAGWQSAMAALEAAGGGILQLPAGVIDIDGALQDTGRANAQLLLPSRHAVNAEQITV
                     VIRGVRPPPTTFSVIGTTPLPDNHSVIRSTLTAGSGGNLLGCRGPAGSFSDFSNVNVV
                     LRDVTFRLPANPTHSGVDLSHVTGCNVDEVVIDTGSYWVQSLTQPTTATSYGIKFPKN
                     NNGADTRIGCLNVFGFYTGAQIAEHTNAEQMNFWGCLRALESAAMTNHASHIKRLMTV
                     HCARGIVASGGAHYLDVEQFNIEHAASGWWAPVYDIDDASNLLRGRVRWHVVLAGVGV
                     DGTFLVNGAEGVEQTRVGFPASPATKSPTTSYTAIITDAGKLIRMNAAAANTFTVPPN
                     SSAAFPVGTELHIQQAGVGATALGAGAGVTISRPASQTLTLKEQYAVATLKKVGTDAW
                     IAFGMLGAV"
     gene            complement(3384..4241)
                     /locus_tag="AC16UZ_06385"
     CDS             complement(3384..4241)
                     /locus_tag="AC16UZ_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246348.1"
                     /translation="MSYTPPASNAVNASWVGDSAYTPPAANAVDASWAPIVPTLVVGS
                     TTHAHAADQPTLEMQVGNYVAPGYVAPGYVEGGSIPSLTIADGTHAHAADAPTLSTEV
                     VLAIADATHAHAAETPALSTAQVLAIGASTHAHTADALALSTDSFLVVSDAAHGHFSD
                     TPALALGFNPQVDGATHAHVAETPTFNLGYQLAIEAALHAHAADSLQLDTNSALAVED
                     ALHTHTADEIGWPAFSVPELLLNHSVALRMAPSDARLRHNTDRGHNLRVYNRAEAVRI
                     RTPGARVRT"
     gene            complement(4238..4531)
                     /locus_tag="AC16UZ_06390"
     CDS             complement(4238..4531)
                     /locus_tag="AC16UZ_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246349.1"
                     /translation="MADLGAIGDEVPVPGVNNAYVFSGTVLDDAGAPAQRLVRLHRRS
                     DGGVIREVMSRADGAYTLPSTLPDEANDQYIVFFDDDAGTQYNHKILGRLTLP"
     gene            complement(4535..>5031)
                     /locus_tag="AC16UZ_06395"
     CDS             complement(4535..>5031)
                     /locus_tag="AC16UZ_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246350.1"
                     /translation="SYQSAHFFGDIVSRVPADQYPFLCVLNTGTSVTNSGHASLSSTI
                     ATSLVGHYIMRPYTAAAGAVAAGKTANHILSAQQSTIGSGGLTYPDPVAGGMILAPLQ
                     LVESSLWRGTIPGMWAPAHALPGDNGDTISGRGAMTGKQFILLDTPSSVSRGRVAIEI
                     SDTW"
BASE COUNT          902 a         1588 c         1610 g          931 t
ORIGIN      
        1 ttccgcacga gtagtagctt gttcgcgttc gcgtacgact cgggtgattc gggcctcggc
       61 cttctcggat tgctcacgtg caaactcagt aacggtgtcc gaatgcgagg cataggcttg
      121 tgcgacctca agctctcggg cggcgcactc gccaccctta aggcggtagc caacccatcc
      181 agatagtgcg cccaccacag acatggcgat agcaacagcg gcgacaacgg catagctcgt
      241 cctcccaatc acaacagaat cccctgctgg tacgacgggc ggccctgcac gaagcgcgca
      301 gtcagtagct gctggcgcgg cgtctcgtgt gtgcgggcga tcccgacgtg gacccacgag
      361 ccctcgtaga tcagctgatc cagtacgacg ccggcctcgg cgatcttgat cgcaacctca
      421 tgcggcgtga gccccaccac cttgaggtcg gcagcgcgac cgtccatgtg ggccgacgtg
      481 cgcgacccac ctatgtggcg gttgagccgc tcgctgcggt agccgctggt gatgatgacc
      541 gggcggttgt ccacggcggt gcggacatcc tccaacgtac gcatgagccg ctccatgttg
      601 gggaccacgc tcggcggcgg tacgttgacg atgccctgcc gctcggcaac ttcggaggcg
      661 gtcagctcgt acagggagaa gtggcgcgtc attcaggcgc ccgcccacgc tggcacgcca
      721 cgcggttgcg acgaaccggg cactcggggc agtgacactc acggcgggtc acgatctgca
      781 gcagcacgcg cccggtgttg gtggtcacga cggagcccag cagcagccac ggccaagcac
      841 gggggtcacc atgcaagtaa tcgaacaccg ccaagatcat gccgaagtgg cccgccgact
      901 tcagaacgta ggcccaccgc acgccattgt gcgtttgcgg gcccatgagg ttcaccgctg
      961 taaggttctg ccacaggatc accagtgaag tactaaccag cccaaccatc agcagggtaa
     1021 cttcgatgct gttcatttgc ggtctcgggg atcgtagggg gtttcggaga agcgccgacc
     1081 cgtcgaagcg gcggcgccga caaggtggat ggagagcagc ccgatgatac agccgacaag
     1141 gccgcccagt ttgaccagtg ctgactccgt gcgcggaatc cagtcgaagg tggcgatcag
     1201 gtaactcacc gccgggtcgg tggcgaggta cgccattacc gggcccgagg ccagcgcgat
     1261 cagccacata cgtacaggca tcgcgccgag gtagctcatc atcagcacgg cgccgaaaaa
     1321 gcccatcgca ggcgggatgg ctttgtgcca aggtgcgtcc attacgcgat tcctatttac
     1381 gtgtaaagac ctgcacggaa gcccaccggc accgcgtggg cgaacgcggc ggcgccgaag
     1441 ttcgccgtca cctgatgcga gggtggagtg ccgcgataga gcgatgcgaa cgcgtagatc
     1501 gtccccgtca gtcctgaaaa tgccggattc gtgccggcgg cggggtcgcc agacgcctgc
     1561 caaaccccat tcttactgaa ccacaccttg ccggaatcca tgtcgagcgc gatgccgatg
     1621 atgtcgccgt ttgtcgccca cggtgccccg aatgtggcac ccacattgtt tgtgtatttc
     1681 tggcctgtgt cttcgtagta accccacccc gatgcgctgt tgcctacgaa cgatgctaga
     1741 tcgaccgcgc tggtgccgac gccaagcagc atgaaaggcg aaacggagcc ggtcaccatt
     1801 gtgacctccc agtagtgctt acccgtcgcc actccaagcg tcgagcgcac cgaccgcagc
     1861 gcctcgtccg tcgttttcgt cgctgtccgg tcaccgttgc tgagtaggat gcccgcgtgc
     1921 ttgttcgccg gatcgaaagt cgcataggtg acccccggtc cggcaggggg tgccagttga
     1981 gccgcgacga tgccggggat cattacactg cccccagcat gccgaaggcg atccacgcat
     2041 ctgtgccgac tttcttgagc gttgctaccg cgtactgctc cttgagcgtg agcgtctgac
     2101 tggctggccg gctgatcgtg acccccgcgc ccgcacctag cgccgttgca ccaacccctg
     2161 cttgttggat gtgcagctcc gtacctaccg ggaatgcagc ggagctgttt ggtggcactg
     2221 taaacgtgtt cgccgcagcg gcgttcatgc ggatcagttt gccggcgtcg gtgatgatgg
     2281 cggtgtagct tgtcgtcggc gacttagtcg ctggggaggc tgggaatccc acccgcgttt
     2341 gctcgacccc ttccgcgccg tttacaagga acgtaccatc cacgccgacg cctgccagta
     2401 cgacatgcca gcgaacgcgc ccgcgcagca gattgctcgc gtcatcaatg tcatacactg
     2461 gcgcccacca acccgacgcg gcatgctcaa tgttgaactg ctcgacatct agatagtgcg
     2521 ccccaccgga ggcgacgatg ccgcgcgcgc aatgcactgt catgaggcgc ttgatgtgcg
     2581 aggcgtggtt ggtcattgcg gcggattcca gcgcgcgcag gcagccccaa aaattcatct
     2641 gctccgcgtt ggtgtgctcg gcaatttggg cgccggtata gaacccgaac acgttcaggc
     2701 atccaattct ggtgtccgcg ccgttgttat tcttcgggaa cttgatgccg tagctcgttg
     2761 ccgtcgtggg ctgtgtcaag ctctgcaccc agtacgaccc ggtgtcaatc acaacctcgt
     2821 caacattgca gccagttaca tgcgaaagat caacccccga gtgcgttgga tttgcgggaa
     2881 gacggaaagt aacgtcgcgc agcacgacat ttacattact gaaatcagag aacgatccag
     2941 cagggccgcg acatcccagc aggtttccgc ccgagcccgc tgtgagtgtg ctcctaataa
     3001 ctgagtggtt atccggtaga ggggtcgtac caataacaga aaatgtcgtc ggcggcgggc
     3061 gcacgccacg gatgacgacg gtaatctgct ctgcgttcac agcgtgtcgt gacggtagca
     3121 gcaactgcgc gttcgctcgc cccgtgtcct gtagggcgcc atctatgtca atcacgcctg
     3181 ccggaagttg caaaataccg ccgcctgctg cttcgagcgc cgccattgcg gactgccagc
     3241 cggcgtagta actcccacta acagtggcat atgttgcgag atcgaacacc ggcagcgccc
     3301 gcgcggcaag ctcggtgaag ttgccatcca gctcggcgtg ggtcagttcg ctgcccttta
     3361 ctgatcggag tacgatcgcc atatcaagtc ctcactcgcg cgcccggcgt gcggatacgc
     3421 actgcctcgg cgcgattata tacccgcagg ttatgcccgc gatcggtgtt atgccgcaat
     3481 cttgcatccg acggcgccat gcggagcgcc acggagtggt tcagtagcag ctcgggcacg
     3541 ctgaacgccg gccagccgat ctcatccgcc gtatgcgtat gtagggcgtc ctcgacggcc
     3601 aaggcgctgt tcgtatcgag ctgcaggctg tccgccgcgt gggcgtgcag ggccgcctcg
     3661 atggccagct ggtagccgag gttgaaggtg ggcgtctctg ccacatgggc gtgcgtggcc
     3721 ccatccacct gcgggttgaa ccccaaggcg agggcgggcg tatcgctgaa gtgcccgtgc
     3781 gcggcgtcgg agacgacgag gaagctgtcc gtcgacaggg ccagtgcatc cgccgtgtgc
     3841 gcgtgcgtac tggcgccgat cgccagtacc tgcgcggtcg acagcgccgg cgtctctgcc
     3901 gcgtgcgcgt gcgtggcgtc tgcgatggcc agcaccacct cggtcgacag cgtcggggcg
     3961 tcggcggcgt gcgcgtgagt gccatcagcg atggtgaggc tggggatgct gccgccctcg
     4021 acgtacccgg gcgcaacata cccgggcgcg acgtagttgc cgacctgcat ctctagggtc
     4081 ggctggtcag cagcgtgcgc atgcgtggta ctgcccacga ccagcgtggg tacgatcggg
     4141 gcccatgagg catcaaccgc gttcgcggcg ggcggcgtat aggcggagtc ccccacccac
     4201 gaggcgttaa ccgcgttcga ggccgggggc gtgtagctca tggcagcgtc aggcggccaa
     4261 ggattttgtg gttgtactgc gtgccggcgt catcgtcgaa gaacacgatg tactggtcgt
     4321 tcgcctcgtc agggagcgtt gagggaagtg tgtaggcgcc atcggcgcgc gacatcacct
     4381 cgcggattac cccaccgtcc gaacggcgat gcaggcgcac gagtcgctgc gcaggcgccc
     4441 ccgcatcatc cagcacggtg ccggagaaga cgtacgcgtt attgactccc ggtacgggca
     4501 cctcgtcgcc gattgctccc aagtcagcca tgtctcacca cgtgtccgag atttcgatgg
     4561 caacccgccc gcgcgacacg ctgctcgggg tgtcgagcag gatgaactgc ttgccggtca
     4621 ttgccccacg gcctgatatg gtgtcgccat tgtcccccgg cagtgcgtgt gcaggcgccc
     4681 acatgccggg gatggtgccc cgccacaggc tggactcgac aagctgcaat ggggccagaa
     4741 tcatgccgcc agcgacaggg tccgggtatg tcaggccgcc ggacccgatg gttgactgct
     4801 gcgcgctcaa aatgtggttg gctgttttcc ccgcagccac tgcgcccgcc gccgccgtgt
     4861 aggggcgcat gatgtagtgc cccacaagtg aggttgcgat tgtgctgctc aagctggcgt
     4921 gccccgagtt cgtgactgag gtgccggtgt tgaggacgca caggaagggg tactgatccg
     4981 ccggcacacg actgacgatg tcgccgaaga aatgggcgct ctggtacgaa g
//
LOCUS       DBNHOX010000292         1709 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1117212, whole genome shotgun sequence.
ACCESSION   DBNHOX010000292 DBNHOX010000000
VERSION     DBNHOX010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1117212"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="AC16UZ_06400"
     CDS             <1..336
                     /locus_tag="AC16UZ_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246351.1"
                     /translation="ANYERPFGRNLVFGMTANLQFRSKTGLSANDPSATYKGYETLDA
                     NVRIGTQDGKWQLAFIGKNLTDKLAIRGATNVPGTGGNTGTAEGFRGDLTGFAIRPRQ
                     YELELTYRY"
     gene            353..850
                     /locus_tag="AC16UZ_06405"
     CDS             353..850
                     /locus_tag="AC16UZ_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6246352.1"
                     /translation="MSVRRVNHTGISVADMERSLTFYRDLLGLELIFDSDVDDVPDLN
                     AVVGMKHARGRVAWLRAGDTMIELWQWDHPRGRPLPKDYRPADRGVTHFALEVDDVDQ
                     LCERIVDAGYHANTTPKDLGLHKTTYVRGPDDEIIEILEDRATDEWLARLTAEAESRR
                     REGDA"
     gene            complement(877..>1709)
                     /locus_tag="AC16UZ_06410"
     CDS             complement(877..>1709)
                     /locus_tag="AC16UZ_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR6246353.1"
                     /translation="SPVSVPTNEGDGLTMSMRIGAALGNMREAWWMPAVEIPGDRGDG
                     RPHQHLFASERARPRSIMVNRAGRRFTNEAANYNAFGAAFHEQDVARFDYANLPCWFI
                     FDQKHLDTYGFIGTPPGAPAANWVANAPTLRALAAQLGIDADGLDKTVAQWNGNVAEG
                     RDPDFHRGESAHDTWWGDPWEKGTPAATLGPVDTGPFYAVEVRSGALGTKGGPRTDGL
                     ARVKNVDGEIISGLYAAGNVMASAMGMTYGGAGGTLAPGMVFGFLAGRDAARRAVGKS
                     "
BASE COUNT          289 a          558 c          580 g          282 t
ORIGIN      
        1 gccaactacg agcggccctt cggtcgcaac ctggtgttcg ggatgacggc caacctgcag
       61 ttccgcagca agacggggct cagcgcaaac gacccgagcg caacgtacaa aggctacgaa
      121 accctcgatg ccaacgttcg gatcgggacc caggacggca agtggcagct ggccttcatt
      181 ggcaagaacc tgaccgacaa gttggccatc cgtggtgcga ccaacgtgcc gggtacgggg
      241 ggcaacactg gcacggccga aggatttcgc ggcgacctga cgggcttcgc gatcaggcct
      301 cgccagtacg agctcgagct gacctaccgc tactaaggtc tcgggagcgt tcatgtctgt
      361 caggcgcgtc aaccataccg ggatcagcgt ggcggacatg gagcgctccc tcacgttcta
      421 ccgcgatctg ctggggctcg agctgatatt cgactcggac gtggacgacg tccccgatct
      481 caacgccgtg gtgggcatga agcatgctcg aggcagggtg gcctggctgc gcgcgggcga
      541 caccatgatc gagctgtggc aatgggatca cccgcgaggc cggccgctgc ccaaagacta
      601 ccggccggcg gacagaggcg tgacccattt cgcgctggag gtggacgacg tcgatcagct
      661 ctgcgagcgc atagtggatg ccggatatca cgccaatacc acgccgaaag atctgggcct
      721 gcacaagacg acgtacgtgc gggggccgga cgatgagatc atcgagatac tcgaagatcg
      781 tgctaccgat gagtggctgg cgcggctgac ggccgaggcc gagtcaaggc gtcgcgaggg
      841 tgacgcgtag gccgaatgtc cgccctgacc ggggcgtcag ctcttcccga cagcccgcct
      901 tgctgcatcg cgtcccgcga gaaatccgaa gaccatgccg ggcgccagcg ttccgccggc
      961 gcctccgtag gtcatgccca tggccgaggc catgacgttg ccggcggcgt acagcccgct
     1021 gatgatttcg ccgtccacgt ttttcaccct ggcgaggccg tcggttctcg ggccgccctt
     1081 ggtgcccagc gcgccgctcc tgacctccac ggcgtaaaat gggccggtgt caacagggcc
     1141 cagcgtcgcg gccggcgtgc ctttctccca gggatcgccc caccaggtgt cgtgggcact
     1201 ctcgccgcgg tggaagtccg gatcgcggcc ttccgccacg ttgccgttcc actgtgccac
     1261 cgtcttgtcc agcccgtcgg catcgatgcc gagctgggca gccagcgctc gcagagtggg
     1321 cgcgttggct acccagttgg cggcgggcgc ccccggcggg gtgccgatga agccgtaggt
     1381 gtccagatgt ttctggtcga agatgaacca gcagggcagg ttggcgtagt cgaagcgcgc
     1441 cacgtcctgc tcgtgaaagg cggccccgaa cgcgttgtag ttggccgcct cgttggtgaa
     1501 acggcggccg gcccgattga ccattatcga ccggggccgc gcgcgctcgg atgcgaacag
     1561 gtgctggtgc gggcggccgt cgccgcggtc accggggatc tccaccgcgg gcatccacca
     1621 ggcctcgcgc atgttgccca gcgcagcgcc gatgcgcatg ctcatggtca acccgtcacc
     1681 ctcgttggtc ggcacggata ccgggctgg
//
LOCUS       DBNHOX010000293         2819 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_538380, whole genome shotgun sequence.
ACCESSION   DBNHOX010000293 DBNHOX010000000
VERSION     DBNHOX010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_538380"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            351..1850
                     /gene="istA"
                     /locus_tag="AC16UZ_06415"
     CDS             351..1850
                     /gene="istA"
                     /locus_tag="AC16UZ_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HIR6246354.1"
                     /translation="MYSVDLYARVRRACRIDGMTKREAARVFGIDRKTVNKMLAFSVP
                     PGYRRQRPPRRPKLDGFTGIIDRILDEDGGVHRKQRHTAKRIFERLRDEHGFTGGYTI
                     VKDYVRERRRRSREMFVPLSHPPGHAQVDFGEALAVIGGVLRKIHFFAFDLPHSDAGF
                     VKAYPAETTEAFCDGHVAAFAFFGGVPLSILYDNTTLAVARILGDGTRQRTRVFSELQ
                     SHYLFGDRFGRPGKGNDKGKVEGLVGSIRRNYLVPVPHVESLDALNDDLATRCRRRLG
                     DRLRRHTETIGERLACDLEAFADLPPVPYDPCDRRPGRVSSLSLVRYRSNDYSVPVAY
                     GHREVLIRGYVDRVTICCGAEEIARHRRSYEREDLIFDPLHYLPLIEQKIGALDQAAP
                     LQGWDLPETFATLRRLLEARMGKAGKREYVQVLRLMETFPLAEVEAAIHDALKLGAIG
                     FDAVKHLVLCRIERRPPRLDLDLYPYLPRARVATTSAKSYMSLLSGAGP"
     gene            1847..2611
                     /gene="istB"
                     /locus_tag="AC16UZ_06420"
     CDS             1847..2611
                     /gene="istB"
                     /locus_tag="AC16UZ_06420"
                     /inference="COORDINATES: protein motif:HMM:NF013828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HIR6246355.1"
                     /translation="MTDAPQLLLNHHLKTLKLPTFVREYDKLARQCASEGVDHVRYLL
                     RLAELELIDRERRMIERRIKAARFPVVKSLDGFDFKAIPSLNKTLVLELARGEYIERR
                     ENVIALGNSGTGKTHIALGLGLAACQKGLGVGFTTAAALVHELMEARDEKRLLRLQKQ
                     LAGYKLLIIDELGFVPLSKTGAELLFEIFSQRYERGSTLVTSNLPFDEWTEIFGSERL
                     TGALLDRLTHHVHILEMNGESYRLNQSRGKKRSAAG"
BASE COUNT          516 a          934 c          885 g          484 t
ORIGIN      
        1 gtcgggggac cagacagacc acatccgggt tactcccaaa aacggaaatc agcgcaccgt
       61 gggcactttt cctccagcca gcagataaag tcccgcaacc accagcacga tcccagccac
      121 gcgtgacagc tctacccgga gattgcgtaa atgcgcgttt tattttccaa acacccacgt
      181 ccgatcagaa aaacgctccc gcgctgtaat cggataaacc ggaacctgag cctcctgaga
      241 agggcggcgc aataatcccc cagtgaggcg gccggatttc cgggctgcgg cggaccaaaa
      301 tggggccgcc tattagcctt cggcaggatt gaactgatcg aaggcggggg atgtattcag
      361 tggatttgta tgcgcgggtt cgacgggcgt gccgtattga tgggatgacc aagcgtgagg
      421 cggcacgggt cttcgggatc gatcgcaaga cggtgaacaa gatgctggcg ttctcggttc
      481 cgccgggcta ccggcggcag cgtccgccgc gacggccgaa gctggatggc tttacgggca
      541 tcatcgaccg catcctggat gaagatggcg gggttcaccg caagcagcgg cacacggcga
      601 aacggatttt cgagcggctt cgggacgagc acggcttcac cggcggctac acgatcgtga
      661 aggactatgt ccgcgagcgc cggcgtcggt cgcgggagat gttcgtgccg ctgtctcatc
      721 cgccgggtca cgcccaggtg gacttcggcg aggcgctggc ggtcatcggc ggagtactcc
      781 ggaaaatcca ctttttcgcc tttgacctgc cgcacagcga cgccggcttc gtgaaggcct
      841 atccggcgga gacgacggag gcgttctgcg acgggcacgt ggcggcgttc gccttcttcg
      901 gcggtgtacc cctctcgatc ctgtacgaca acaccacgct ggcggtggcg cggatcctgg
      961 gtgacggcac gcgacagcgc acccgggtgt tcagcgagct gcagtcccat tacctgttcg
     1021 gggaccgctt cggccgtccc ggcaaaggca acgacaaggg caaggtggag ggtctggtcg
     1081 gctccatccg gcgcaactac ctggtgccgg tgccccatgt cgagagcctg gacgctctga
     1141 acgacgatct cgcgacgcga tgccgtcggc ggctcggcga ccgattacgc cgtcacaccg
     1201 agacgatcgg cgaacggctc gcgtgtgatc tcgaagcgtt cgcggatttg ccgccggttc
     1261 cctacgatcc ctgcgatcgc cgtcccggac gggtgagctc gctgtcgctg gtccgctaca
     1321 ggagcaacga ctactccgtg cccgtcgcct acggtcaccg tgaggttctt atccggggct
     1381 acgtcgaccg ggtgaccatc tgctgcggcg cagaggagat tgcccggcat cggcgctcct
     1441 atgagcgcga agatctgatc ttcgatccac tgcactacct gccgctgatc gaacagaaga
     1501 tcggcgcgct ggaccaggcc gcgccgctcc aaggctggga cctgcccgag accttcgcca
     1561 cattgcgccg cctgctggag gcgcgcatgg gcaaggccgg caagcgggag tacgtccagg
     1621 tgctgcgcct gatggagacc ttcccgctgg ccgaggtcga ggcggcgatc cacgatgccc
     1681 tgaagttggg cgccatcggc ttcgatgccg tcaagcacct ggtgctgtgc cgcatcgagc
     1741 gccggccgcc gcggctcgac ctcgacctct atccctatct cccgagggcc agggtcgcga
     1801 ccacgtcggc caagagctac atgagcctgc tgtcaggggc cggcccatga ccgacgcgcc
     1861 acagctgttg ctgaaccacc acctcaagac gctcaagctg ccgacgttcg tccgcgagta
     1921 cgacaagctc gcccggcagt gcgcttcgga aggcgtcgat cacgtgcgct atctgctgcg
     1981 tctcgccgag ctcgagctga tcgaccgcga gcgccgcatg atcgagcgcc gcatcaaggc
     2041 cgcccgcttc cccgtggtca agagcctgga cggcttcgac ttcaaggcca tcccctcgct
     2101 caacaagacg ctggtgctcg agctcgcccg tggcgagtac atcgagcgcc gcgagaacgt
     2161 catcgcgctc ggcaacagcg ggaccggcaa gacccacatc gccctcgggc tcggcctggc
     2221 ggcctgccag aagggcctcg gcgtcggctt caccaccgcc gccgccctgg tgcacgagct
     2281 catggaggcg cgcgatgaga agcgactgct gcgcctgcag aagcagctcg ccggctacaa
     2341 gctcctgatc atcgacgaac tcggcttcgt gccgctgtcc aagaccggcg ccgagctcct
     2401 gttcgagatc ttcagccagc gctacgagcg cggctcaact ttggtgacct cgaacctgcc
     2461 gttcgacgaa tggaccgaga tattcggctc cgagcgcctg accggcgcgc tgctcgaccg
     2521 cctcacccat cacgtccata tcctcgagat gaacggcgag agctaccggc tcaatcagag
     2581 ccgggggaaa aagcggtccg ccgccggctg acccaccacc tcgcctctgc ttgtccccgt
     2641 ggactacggg ggcaagccta aaacgccgcg tggcccaatc ttacgccgcc caatggcccc
     2701 tttttactcc gccgttgaca acacgcattt gaacccgaga cgcatcaggc gaaagttcgt
     2761 tgagatcgtg gacgctctcg aatcccaaga ttacttagag ggcgttaaag ggttcaacg
//
LOCUS       DBNHOX010000294         2204 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_795127, whole genome shotgun sequence.
ACCESSION   DBNHOX010000294 DBNHOX010000000
VERSION     DBNHOX010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_795127"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1436)
                     /locus_tag="AC16UZ_06425"
     CDS             complement(<1..1436)
                     /locus_tag="AC16UZ_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024973.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HIR6246356.1"
                     /translation="MSRRLIVLLTCLFAPWAWSAIQTSVEPRLLDEMDTLRLTLRSTG
                     QSRADTLDLSPLDEDFEVLGTNTQSQYRSVNGRIDSWVEHQIDLRPRRTGELLVPAIS
                     IGGEQSEAIRIVVRPMDPGVRRAIDRMVFFETELSSNPVYVQGETVFTRRLYYSSGVQ
                     IYSDLPGAPEVPDAVVIPVGETRSSSTLVGGQRYGIVEQQFAIFPERSGAVVIPETSI
                     TSSVRMQTGGRYRRSGIRVSTEPIELEVLPIPPAYPQHEPWLPATDVQLSEAWSPSTT
                     EITVGDPVERRFTLVATGNIGSAIPPIDAKFPEGRLKSYPQSPEILDDTRGSSVVGTR
                     QEVYSLIAVAGGEVAFPPVEVTWWDTGTERVRKSRLDGRMVRIAGGRPALPQKPLAED
                     PVSDASGVGAEPAAAAGSDLEPGLGLNPLHAAAILFAFLLLAGFWALSGRFRSSAPDA
                     GPTSRQAWASLRRSCRNQEPQVLRAALL"
     gene            complement(1433..>2204)
                     /locus_tag="AC16UZ_06430"
     CDS             complement(1433..>2204)
                     /locus_tag="AC16UZ_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246357.1"
                     /translation="LIPLALLGFRRGLLAAVCIGILPLPAEANLWDDLWQRRDQQAVE
                     MLEQGDPAAAANLFEDPDWRAAARYRAGDYPGAAEAFAAQLSATGIYNLGNALAHQGD
                     LQTAIAAYSEVLAQQPGHEDAAFNKALLEELLEQQQSQQQQDQNQQDDGQGSEDDQST
                     PSGSGEQQDQTAEQDPGEQSDRPSGQSASGDEPSEPAPGSETELADARDEQQDALEQW
                     LRRVPDDPGGLLRRKFRYETNQRLRRGEYQSNPSEQIW"
BASE COUNT          344 a          723 c          750 g          387 t
ORIGIN      
        1 tgcagcagcg cggcgcgcag gacctggggt tcctggttcc ggcagctgcg gcgcagcgac
       61 gcccaggcct gcctcgaggt cgggcccgcg tcgggcgcgc tggatcggaa acgtccggag
      121 agagcccaaa acccggccag cagcaggaac gcgaagagga tggcggctgc gtgaagcggg
      181 ttcaacccaa ggccgggctc gagatccgag ccggctgctg ccgcgggctc ggcgccgacg
      241 cctgacgcat cggaaacagg gtcttcagcg agaggtttct ggggcaaggc gggtcttccg
      301 ccggcaatgc gcaccatgcg gccatccagc cggctcttcc tcacgcgttc ggtcccggta
      361 tcccaccacg ttacctctac cggaggaaat gccacctcgc cgccggcgac ggcaatcagc
      421 gagtaaacct cctggcgggt gccgacgacg ctgctgccgc gggtgtcgtc caggatctcc
      481 ggagattgag gataggattt caggcggcct tcagggaact tcgcgtcgat cggcgggatc
      541 gcggagccga tgttgcccgt ggcaaccagg gtgaagcggc gttccaccgg gtcgccgacg
      601 gtaatctcgg tggtgctggg cgaccaggct tcggaaagct ggacgtcggt tgcgggcagc
      661 cacggctcgt gctgggggta ggccggcggg atgggaagca cttccagctc gatgggttcg
      721 gtgctgaccc ggataccgga gcggcggtag cgcccgccgg tctgcatgcg gacgctggac
      781 gtgatggacg tctccgggat gacgaccgcg ccagagcgct cgggaaatat ggcgaattgc
      841 tgctctacga tgccgtagcg ctgcccgccc acgagagtgc tgctcgatcg cgtctcgccc
      901 accggtatga ccaccgcgtc cggcacttcc ggggccccgg gcaggtcgct gtatatctgc
      961 acgccgctcg agtagtagag cctgcgcgtg aacacggtct cgccctgaac gtataccggg
     1021 ttgctggaca gctcggtctc gaagaagacc atgcgatcga tggcacgcct cacgcccggg
     1081 tccatgggac gcaccacgat tcggatcgcc tcgctctgct cgccgccgat ggagatcgcc
     1141 ggcaccagca actcgcccgt tcgacgtggc cgtagatcga tctggtgctc gacccagctg
     1201 tctatgcgtc cgtttacgga gcggtactgg ctctgggtat tggtgccgag cacctcgaaa
     1261 tcctcatcga gcggcgacag gtccaatgtg tccgcccggc tctggcctgt ggagcgcaaa
     1321 gtcagccgaa gggtatccat ctcgtcgagc agccgaggct ccacggaagt ctggatggct
     1381 gaccatgccc aaggtgcgaa caggcaggtg agcaggacga tgagtcgccg gctcaccata
     1441 tctgctccga ggggttgctt tggtactcgc ctctgcgcag tcgctgattg gtttcgtagc
     1501 ggaacttccg gcgcagaagg ccgcccggat cgtcgggcac ccggcgcagc cactgctcca
     1561 gcgcatcctg ctgctcatcg cgagcgtcag ccagttccgt ctccgacccc ggagccggct
     1621 cgctgggctc gtcgccggac gccgattgcc ccgaaggccg atcgctctgc tccccaggat
     1681 cctgctctgc ggtctgatcc tgctgctcac cggaaccgga gggcgtggac tggtcatctt
     1741 ctgatccttg cccgtcgtct tgctggttct gatcctgctg ctgttgcgat tgctgctgct
     1801 ccagcaactc ctcgaggagg gctttattga aggccgcatc ttcatggccg ggctgctgcg
     1861 ccagcacctc gctgtaggcg gcgatggcgg tctgcagatc cccctggtgc gccagggcgt
     1921 tgcccaggtt gtagatgccc gttgccgaca gctgggcggc aaacgcctcc gccgcccctg
     1981 ggtagtcgcc tgcccggtat cgggcggcgg cgcgccagtc gggatcctcg aacaggttcg
     2041 cagcggccgc cgggtcgccc tgctcgagca tctccacggc ctgctgatcc cgccgttgcc
     2101 acaaatcgtc ccagaggttt gcctcggcgg gcagcgggag gatcccaatg cacacggcgg
     2161 ctagcagacc gcgccggaaa ccgagcagcg cgagcgggat gagc
//
LOCUS       DBNHOX010000295         3262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_795135, whole genome shotgun sequence.
ACCESSION   DBNHOX010000295 DBNHOX010000000
VERSION     DBNHOX010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_795135"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="AC16UZ_06435"
     CDS             <1..634
                     /locus_tag="AC16UZ_06435"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR6246358.1"
                     /translation="DLLNARDYDVVYARNEGCCGSLNLHLAEKERALADARRLLDVVA
                     EDLEEVEAVISTASGCGVTLKDYPRLLADDPAYADRARALAGKLVDAAEFFASRELEL
                     ERAFDGLRVAWQAPCTLQHGQKITGMVEKVLGAAGYELVSVVDPHLCCGSAGTYSILQ
                     PEMAGELRRRKLATLCAQTPDVIATANVGCQTHLAAESPVPVRHWLELLR"
     gene            716..1117
                     /locus_tag="AC16UZ_06440"
     CDS             716..1117
                     /locus_tag="AC16UZ_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6246359.1"
                     /translation="MKITRINHAAVNVTGKVEEAHDFYVKLLGLPQVPIQLPGAPPVM
                     ESALGFWVEQDGVQLHVIGREPFGGTPDPTQNHVSWFVEDLDVAVGELEQNGIEMRVM
                     GEGSGRIIWVLDPGGNIVEFQQDPDLAPPPA"
     gene            1139..2290
                     /locus_tag="AC16UZ_06445"
     CDS             1139..2290
                     /locus_tag="AC16UZ_06445"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6246360.1"
                     /translation="MRFESRRESFAELESARGLVPGIRTSQCEIDKQQALPADLAARL
                     AADGMFRLLLPVCIGGSGLSLPRFVRVVEVIAEADGSTGWCVSQAAVFASVADLLHAE
                     TAAEIWGTNRDAAVATGIPFGAEARAVDGGYLLDGRWRFASGCTHADWFAPLASVVGD
                     VGSEPSRTFLVPREEVRLGEGWNVRGMQGTGSREYRLHALHVPSRRSVADAYTSHPGA
                     STGLHVMLLFACGFAAVGLGVARRAVDEFIALAAGKTPVFANRKLAEDDLVQSGLGEA
                     EALLGATRAFLLDAAEECEHRHATTGAVDFELRSRMRLAATHAMRRSADVVDRVYAMA
                     GTDCIFDDHPLQRCFQDIHALTQQVQGRASHYRSVGRVLLGRQKETFAF"
     gene            complement(2307..2768)
                     /locus_tag="AC16UZ_06450"
     CDS             complement(2307..2768)
                     /locus_tag="AC16UZ_06450"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6246361.1"
                     /translation="MTAGCATSSVLPPVVDTNGIEDAEPAPQQEVPIPPASTPEARQP
                     DTAGATLALLEQSERAASAGDADAAVAYVERAIRLNPRSADLWLQLARLQLANEDPEA
                     AVQFANKAISLAGDRLDWVRDAWLLIADARAAQGDGDGAREIRERWRGYRG"
     gene            complement(2792..>3262)
                     /locus_tag="AC16UZ_06455"
     CDS             complement(2792..>3262)
                     /locus_tag="AC16UZ_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR6246362.1"
                     /translation="EQRIDTKTARALDRALQVVMARGTGRSSRFARVGVAGKTGTSDD
                     YRDSWFAGYDDAALTVVWVGRDDNAPTGMTGASGALKVWDRIMADRDVIAIPPDPDSR
                     KVDVEFATGLVANPLCADVVTIPVPEGAALQEKPGCGIKPPSLGERIRNWLRND"
BASE COUNT          490 a         1043 c         1133 g          596 t
ORIGIN      
        1 cgatctgctc aatgccaggg actacgacgt cgtctacgcc cgcaacgaag gctgctgcgg
       61 cagcctgaat ctgcatcttg cggaaaagga acgggccctg gcggacgccc gccgcctgct
      121 cgatgtcgtt gcagaggacc ttgaagaggt ggaagcggtc atatccacgg ccagcggctg
      181 cggcgtgacg ctcaaagact acccccggtt gctggcggac gaccccgctt acgctgatcg
      241 cgcgcgggct ctggcgggaa agctcgtcga cgccgccgag ttctttgcct cccgggagct
      301 ggagctcgaa cgtgccttcg atggcttgcg agtggcatgg caggcgccgt gcaccctgca
      361 gcacgggcag aagatcacgg ggatggtgga gaaggtcctc ggcgcggccg gctacgaact
      421 cgtctccgtt gtggaccccc acttgtgctg cggctcggcc gggacctact ccattctgca
      481 gccggagatg gccggcgagc ttcgccggcg caagctcgcc acgctgtgcg cgcagacgcc
      541 cgacgtgatc gcgacggcca acgtgggttg ccagacccac ctggccgcgg agagccccgt
      601 gcccgttcgc cactggctgg agctcctgcg ctagggcccg tccatgggag cggggattgc
      661 ggtggtaggc gcagtccact taaatgaaac gcgaggtggc ataaagggga gattcatgaa
      721 gattacgcgt atcaaccatg cggcggtgaa cgtcaccggc aaggtggaag aggctcacga
      781 tttttacgtc aagctgctcg ggttgcccca ggtgcccatt cagctgccgg gcgcgccgcc
      841 ggtcatggag tctgcgctcg gtttctgggt ggagcaggat ggcgtgcagc tgcacgtcat
      901 cggccgcgag cccttcggtg ggacgcccga tccgacccag aaccacgttt cctggttcgt
      961 ggaggatctg gatgtggcgg ttggcgagct cgagcagaac ggcatcgaga tgcgggtgat
     1021 gggcgagggt tcaggtcgga tcatctgggt tctcgacccc ggcgggaaca tcgtggagtt
     1081 ccagcaggac ccggaccttg ctcctccccc ggcctgatcg tactggcgga aagtcctcgt
     1141 gaggttcgaa agccgccggg agtcgtttgc cgagctcgag agcgccaggg gattggtgcc
     1201 cggcatacgg acctcgcagt gcgagatcga caagcagcag gcgctgccgg ctgacctcgc
     1261 cgctcggctt gccgctgacg gcatgttccg actgcttctg ccggtctgta tcggcggttc
     1321 cgggttgtcg ttgccgcggt tcgtgcgcgt ggtggaagtg attgccgagg cggacggcag
     1381 cacgggctgg tgcgtttctc aggcggcggt gtttgccagc gttgccgatc tgctgcatgc
     1441 ggaaacggcc gctgagatct gggggaccaa ccgcgacgct gccgttgcca caggcattcc
     1501 gttcggtgcc gaggcccgag cggtcgatgg cggctacttg ctcgatggcc gctggcgttt
     1561 tgccagtggc tgcacccatg ccgactggtt tgcacccctg gcctccgtgg ttggcgatgt
     1621 cgggagcgag ccgtcgcgca cttttctcgt cccccgcgag gaggttcgtc tcggcgaagg
     1681 atggaacgtg cgcgggatgc aaggaacggg cagtcgtgag taccggttgc atgcgctgca
     1741 cgtgccgagc cggcgatccg ttgcggatgc ctacaccagt catcccggtg cctccaccgg
     1801 gctgcacgtc atgctcctgt tcgcctgcgg gttcgcggcg gtggggctgg gcgttgctcg
     1861 acgggcagtg gatgagttca tcgccctggc cgccggaaag acgcccgtgt ttgcgaaccg
     1921 gaagctggcc gaggacgacc tcgtgcagag cgggctcggc gaggcggaag cgctgctcgg
     1981 cgccacccgg gctttcctgc tggatgccgc cgaggagtgc gagcatcgcc atgcaaccac
     2041 cggagccgtc gatttcgaac tcagatcccg gatgcgcctt gccgcgaccc acgccatgcg
     2101 tcgctcggcg gacgtggtcg atcgggttta cgccatggca ggcacggact gcatcttcga
     2161 cgaccacccg ctgcagcgct gctttcagga cattcacgcg ctgacccagc aggttcaggg
     2221 cagggcttct cactatcgtt cggtgggtcg tgtgctgctt ggcaggcaga aagagacatt
     2281 cgctttctga ggacccaggg ccctgctcaa ccgcgatagc ctctccagcg ctcgcggatc
     2341 tccctggcgc catccccatc gccctgagcc gctcgggcgt ctgcgatcag cagccaggca
     2401 tcgcgtaccc agtcgaggcg atcgccggcc aggctgatgg ctttgttggc gaactgcacg
     2461 gccgcttccg gatcctcgtt ggcgagctgc aagcgggcga gctgcagcca gaggtcggcg
     2521 ctgcggggat tcaggcgaat ggccctctcc acgtaggcga cggcggcatc cgcgtcgccg
     2581 gccgatgcgg cgcgctcgct ctgctcgagc agcgccaggg tggccccggc cgtgtccggc
     2641 tgccgggcct cgggggtgct cgccggtggg atcgggactt cctgctgggg cgccggctcc
     2701 gcgtcctcga tgccgttggt gtccaccacg ggaggcagca cggaggacgt ggcgcaacct
     2761 gcagtcacga tcagcgtggc caggcctggg atcagtcgtt tcgtagccaa ttgcgaatgc
     2821 gctcacccaa agacggcggc ttgataccac agcccggttt ttcctgtaaa gcagctccct
     2881 cgggaacggg tatggtgacc acgtccgcgc agagcgggtt ggccacgagg ccggttgcga
     2941 attccacgtc caccttccgc gaatccggat ccggcggaat ggcgatcacg tcgcgatccg
     3001 ccatgatccg gtcccagacc ttgagcgcgc cggacgcgcc ggtcatgccg gtcggcgcgt
     3061 tgtcgtcgcg gcccacccat accactgtca gcgcggcgtc atcgtatccg gcgaaccagc
     3121 tgtcacgata gtcgtctgag gtgcccgtct tgcccgctac gccgaccctg gcgaagcgtg
     3181 agctacgccc tgttccgcgc gccatgacga cttgcagcgc ccggtcgagg gctcgcgccg
     3241 ttttcgtgtc gatgcgctgc tc
//
LOCUS       DBNHOX010000296         6744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1053249, whole genome shotgun sequence.
ACCESSION   DBNHOX010000296 DBNHOX010000000
VERSION     DBNHOX010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1053249"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="AC16UZ_06460"
     CDS             <1..750
                     /locus_tag="AC16UZ_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HIR6246363.1"
                     /translation="TGGTVISEEVGLTLEGATLDDLGTAKRISSTKENTTIVDGAGAK
                     GDIEGRIKQIRSEIEDTSSDYDREKLQERLAKLAGGVAVIKVGAPTEVEMKEKKARVE
                     DALHSTRAAVEEGVVAGGGVALIRALSAIEGLKGDNEDQNVGIAIAPRAMTAPLRQIA
                     ANSGDEGAVVLDKVRALTGNEGYNAATGEYGDMLAMGILDPAKVTRTALQAAASVAGL
                     MITTEAMVAEAPDDSPSPGMPGGMPDMGGMM"
     gene            910..1581
                     /locus_tag="AC16UZ_06465"
     CDS             910..1581
                     /locus_tag="AC16UZ_06465"
                     /inference="COORDINATES: protein motif:HMM:NF017945.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HIR6246364.1"
                     /translation="MDQAFIDFLARWSHVVFGITWIGLLYYFNFVQTEYFKEADPDAR
                     TDAFSKLAPRALWWFRWGAMFTFLTGLIMLGVRGAGVTLSITLGSVLGTLMFLNVWLI
                     IWPNQKIVIASNQQVKAGGEADPAAAGAAPKAAIASRTNTLFSIPMLFFMVSSAHLAT
                     NTGIGFGMIGDASMTGLVVSLLIIAALELNGIFGKQGPMTTVRGVIGSSLVLTAVLWA
                     VAALL"
     gene            1716..2462
                     /locus_tag="AC16UZ_06470"
     CDS             1716..2462
                     /locus_tag="AC16UZ_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246365.1"
                     /translation="MADRPPSEAISATPDDASGYRRGASGRRRGAVAEGPRMIGMSFI
                     MAVLVAGLVVAGWFIANQHQLLAAEQRALDDAEKRLVVLEDRLRVTDEAMTETGQDTR
                     EQIGFWESEIRKLWAVANERNRKWIKDNQVALGRLQKTLDELKGSNAELAGAVGRHET
                     AFSQQQAIIDQLAGIDLQMQRMVAGQRDLADKINVAQQTVAGLQSGLTGRVSENEQAV
                     AAIDAYRLQLNSRLADIERQLNQLMSSANL"
     gene            complement(2489..3121)
                     /locus_tag="AC16UZ_06475"
     CDS             complement(2489..3121)
                     /locus_tag="AC16UZ_06475"
                     /inference="COORDINATES: protein motif:HMM:NF027851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HIR6246366.1"
                     /translation="MSYILDALKKSEIENDPAAAASLALTQQRESARNRTIAALVVVA
                     LLANAAVLLWIFRPDPGVQQEPVGPSVAAAQAEPVTVPGQRSEPVTPAPTPPEVSTPG
                     EVATTDVPVINPSPPQPPAREPARRPPARIRTQLADLPRDPKSRFPGLAFSTHVYAED
                     ALLRAVVANGTRLQEGDRLNEVTVEEITEDGVIVAFENYLVEIPVIESWQ"
     gene            complement(3124..4908)
                     /locus_tag="AC16UZ_06480"
     CDS             complement(3124..4908)
                     /locus_tag="AC16UZ_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6246367.1"
                     /translation="MYQDYFQLNQAPFSIAPDPNFLYLSAGHSEALAHLMYGFSHGGF
                     VLITGEVGTGKTTLLRNLIKQTPPDLDVAFILNPRLTVKELLETLCDELGVRYPEGSN
                     PTVKQYIDVLNRHLLAAHEKGRSTVVIIDEAQNLSPAVLEQIRLLTNLETDERKLLRI
                     ILLGQPELGALLDRAELRQLAQRITARYHLGALNREDTYAYIVHRLARAGGNPKLFSR
                     GALSRLFRISKGVPRLINVVADRALLGAYVEGRHQVTGRIVSRAAREVLGRRPRYRLW
                     IGVGAASVSAAGVAWAYLTLPEVVDLPTTIPAVSSPAVQAPSEPVPVPETTLAEPVPA
                     AITPPARAPSLTSRADGVDGAGSQAASPSETEPAPVPVPEIAPEIAPEIAASVAVEPD
                     IRRPPMSSFQSQRRAYAAVFDAWGADYRTAPPSMIPCNFAPSAGLQCLGLRGTWSDIE
                     AMNLPVVLELWDSERAPYFAAVTHIHDGNMTLHLGDTVLTVTPKALRDVWFGQYVVLW
                     QTPPQYRGSISRGNTHPTVSWLRVQLEALVSDDLSSGRPEYFDPRLDEAVRDFQGSEG
                     LLTDGIVGPATWIRLAARLDLPAPKLDG"
     gene            complement(4908..5789)
                     /locus_tag="AC16UZ_06485"
     CDS             complement(4908..5789)
                     /locus_tag="AC16UZ_06485"
                     /inference="COORDINATES: protein motif:HMM:NF010621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIR6246368.1"
                     /translation="MAFLKGILATTLVALNTIVLCIPLFLVSAVRALSPAAIKRWLSR
                     KLDRWIIDGWVATNRACFGWLGLCDVEVQWQGAERLDRDRWYLVISNHQSWTDILLLQ
                     TSLYGHIPPIKFFTKRELIWIPFIGLAMRLLGFPFVRRMTREQIAKKPELVRADRDAT
                     LAACQEFRNHPTTVLNFLEGTRFTPAKQARQDARFKHLLNPKIGGLSYVLSGLSKELD
                     RLVDVTITYPRGTPTFWEFMQGKCPAAHLLVQCREIPQDVRAPGNDHEQREVVSPWIE
                     ALWREKDARLGAQTVAL"
     gene            5883..>6744
                     /locus_tag="AC16UZ_06490"
     CDS             5883..>6744
                     /locus_tag="AC16UZ_06490"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR6246369.1"
                     /translation="MTIGRLLAAAAAASFLSGCASLGYYTQAMSGQLQISSSGQPVGE
                     LLARLEAVEDPDALQAGLLSGLRSSQSVLAFAEAELGLEAGGSYSSYVELDRPYVVWN
                     VFAAPELSLEARSWCYLVVGCAPYRGYFEQGRAERFAAGLKDRGLDVYVGGVPAYSTL
                     GWFEDPLLSTFIRWPQGRLAELLIHELAHSRVWVAGDVGFNEAFASFVGREGARSWIE
                     QRHGAQAADEFLERGRSWPALMRLLLEIRRALEELYISGLSEGEKRAGKSKVIDRGKA
                     CYQRNISRYGS"
BASE COUNT         1144 a         2109 c         2281 g         1210 t
ORIGIN      
        1 accggcggca cggtgatctc cgaggaggtt ggcctgacgc tggaaggcgc gacgctggac
       61 gacctcggga ccgccaagcg catctcctcc accaaggaga acacgaccat cgtcgacggt
      121 gcgggcgcga agggcgatat cgaaggacgc atcaagcaga tccgctccga gatcgaggac
      181 accagctccg actacgatcg cgagaagctg caggagcgtc tggccaagct ggccggcggt
      241 gtggcagtga tcaaggttgg cgcgcccacg gaagtggaaa tgaaggagaa gaaagcccgt
      301 gtcgaggacg cgcttcactc cacgcgcgcc gccgtcgagg aaggtgtggt tgcaggcgga
      361 ggcgtcgcgc tcatccgcgc tctgtcagcc atcgaaggcc tcaagggcga taacgaagac
      421 cagaacgtgg gcattgccat tgccccgcgt gccatgaccg cgccgctgcg ccagattgcc
      481 gccaactccg gcgacgaggg tgccgtggtg ctcgacaagg tccgcgcact caccggcaac
      541 gaaggctaca acgccgccac cggtgagtac ggcgacatgc ttgccatggg tatcctggat
      601 ccggccaaag tgacccggac ggccctgcag gctgccgcgt ccgtagccgg cctcatgatc
      661 accaccgagg caatggtggc cgaagcccct gacgacagcc cgtctccggg gatgcccggc
      721 gggatgcccg acatgggtgg catgatgtaa agttccgagc tggaacgaaa gcccctcccc
      781 aggaggggct tttttttgcc tggctccagg gaccggggag cctcatggtc aaggtgattt
      841 ctggaggggg tttcgctata ttcgagctca gagagacgct aaatccatcg cttttgggga
      901 gaaaacaaaa tggatcaggc ctttatcgac tttctcgcgc gctggagcca cgtcgtgttc
      961 ggcattacgt ggatcggctt gttgtactac ttcaacttcg tccagacgga atacttcaag
     1021 gaggcggatc ctgacgcgcg cacggatgcc ttttccaagc tggcccccag ggcactgtgg
     1081 tggttccggt ggggcgccat gttcaccttc ctcacgggtc tgatcatgct gggcgttcgc
     1141 ggcgccggcg tgaccctctc gattaccctc gggtcggttc tcggaacgct tatgttcctc
     1201 aacgtctggc tgatcatctg gcccaaccag aagatcgtca tcgcctccaa ccagcaggtc
     1261 aaagccgggg gcgaggcaga cccggcggcc gcgggggcgg cgcccaaggc ggcgatagca
     1321 tcccggacca acacgctgtt ctcgatcccc atgctcttct tcatggtttc gagcgcgcac
     1381 ctcgccacca acaccggcat cggcttcggg atgatcggtg atgcgagcat gaccggtctt
     1441 gtggtttccc tgctcatcat cgccgcgctg gagctcaacg ggatcttcgg caagcagggt
     1501 ccgatgacca cggttcgcgg cgtgatcggc tcgagcctgg tgctgaccgc cgttctctgg
     1561 gcggtggccg cactgttgta ggcggcccga cggcccgtcg ggtgaaagat cggagagcct
     1621 gcacttgcag gctctttttt ttgctgtaac ttccgtgccc tgaaccgccg tccggcctgg
     1681 ccgattggcc tcagcgtatc tggagacaca ccaacatggc agacagaccc ccatcggagg
     1741 ccatatcagc gaccccggac gatgcatccg gataccgtcg cggcgcttcg ggaaggcggc
     1801 ggggtgcggt ggccgaaggc cccaggatga tcggcatgag cttcatcatg gcggttctgg
     1861 tggccggcct cgtcgtggcg ggatggttca tcgccaacca gcatcagctg ctcgccgccg
     1921 agcagcgggc cctcgatgac gcagagaaac ggctcgtggt cttagaagac cgtctcaggg
     1981 tgaccgacga agcgatgacg gaaaccggcc aggatacccg ggagcagatc ggtttctggg
     2041 aatctgagat acgtaagctc tgggccgtag ccaacgagcg caaccgcaag tggatcaagg
     2101 acaaccaggt cgcgctcggg cgcctgcaga aaacgctcga tgagctcaaa gggtcgaacg
     2161 ccgagcttgc cggtgcggtt gggcgccacg agacggcctt ctctcagcaa caggccatca
     2221 tcgaccaact cgccggcatc gatctccaga tgcagcgcat ggtcgccgga cagcgagacc
     2281 tggcggacaa gatcaatgtt gcgcagcaga cggtggccgg cctgcagtcg gggcttaccg
     2341 gccgggtcag cgagaacgag caggcggtcg ccgccatcga cgcgtatcgg ctgcagctga
     2401 attcccggct cgccgacata gagcgccagc tcaaccagct catgtcctcg gcaaacctgt
     2461 gacgttctga gagggccttt cgccgcccct actgccagga ctcgatgacg gggatctcca
     2521 cgaggtagtt ttcgaacgcc acgatgacgc cgtcctcggt gatctcctcc accgtcacct
     2581 cgtttagccg gtcgccttcc tgcaggcggg tgccgttggc gaccacggca cgcagaaggg
     2641 catcctcggc gtagacgtgg gtcgagaacg ccagtcccgg aaagcggctc ttcggatctc
     2701 tcggcaggtc ggccagctgc gtcctgattc ttgcgggtgg ccgccgtgcg ggctcccggg
     2761 cgggcggctg gggtggcgac gggttgatga cgggaacgtc ggtggtagca acctcgccgg
     2821 gggtgctgac ctcgggtggg gtgggcgcgg gcgttaccgg ctcgctgcgt tgccccggca
     2881 cggtcacggg ctccgcctgg gcggcggcca ccgatggacc gaccggctct tgctggaccc
     2941 cggggtcggg tcgaaagatc cacagcagca cggctgcgtt ggccagcagg gcaactacga
     3001 cgagggccgc gatggttcgg ttgcgcgcgg attcccgctg ctgggtgagc gccaggctcg
     3061 ccgctgctgc cgggtcgttc tcgatctcgg attttttcag cgcatcgaga atgtaagaca
     3121 ctgtcagccg tccagcttcg gagccggaag gtccaggcgc gcggccaggc ggatccacgt
     3181 ggcaggcccg acgatgccgt cggtcagcag gccctcggaa ccctgaaagt cgcgcaccgc
     3241 ttcgtcaagc cgggggtcga agtattcggg tcgcccgctc gacagatcgt cgcttaccag
     3301 ggcctccagc tgaaccctga gccagctgac agttgggtgg gtattgcctc ggctgatgct
     3361 gccccggtac tgaggcggcg tctgccagag caccacgtac tggccgaacc agacatcgcg
     3421 cagcgctttg ggcgtcaccg tgaggacggt gtcccccagg tgcagggtca tgttgccgtc
     3481 gtgtatgtgc gttaccgcag cgaagtaggg cgccctctcg gaatcccaca actccagcac
     3541 cacgggcagg ttcatggcct cgatatcgct ccaggtgccg cgcaggccca ggcattgcag
     3601 gcccgctgac ggcgcgaagt tgcatgggat catgcttggc ggggctgtgc ggtaatcggc
     3661 gccccaggcg tcgaacacgg ccgcgtaggc cctgcgctgg gattgaaagc tgctcatcgg
     3721 cggccgccgg atatcgggct cgacggctac gctggccgcg atctccggcg cgatctccgg
     3781 cgcgatctcc ggcacgggca ccggcgcggg ctcggtctcg ctgggcgacg cggcttggga
     3841 gccggcgcca tcgacgccat cagcccgcga cgtcaggctt ggggcccgtg ctggtggcgt
     3901 aatcgctgcc ggtacgggct cggcaagggt ggtttcgggc accggcaccg gctcgctcgg
     3961 ggcctgcacg gccggtgacg agacggccgg aatcgtcgtc ggcagatcga caacctctgg
     4021 cagggtgagg taggcccagg cgacgccggc ggccgagacc gaggcggcac cgacgccgat
     4081 ccagaggcga tagcgcggcc ggcggccgag cacctccctg gccgcgcggc tgacgattcg
     4141 tccggtgacc tggtgtctgc cctccacgta ggcgccgagc agcgcccggt cggccaccac
     4201 gttgatcagc ctgggaacgc ccttcgagat cctgaacagg cgtgaaagcg cgccccggga
     4261 aaacagcttc gggtttccgc ccgcgcgggc caggcggtgc actatgtacg cgtaggtgtc
     4321 ctcccggttg agcgccccca ggtgatagcg tgcggtgatg cgctgggcga gctggcgaag
     4381 ctcggcccgg tccagcaggg cgccgagttc gggctggccc agcaggatga ttcgcagcag
     4441 ctttctctcg tccgtctcca gattggtcag caggcggatc tgttccagca ccgccggtga
     4501 gaggttctgg gcttcgtcga tgattaccac cgtgctgcgc cccttctcgt gggccgccag
     4561 aaggtgccgg ttgagcacgt cgatgtattg cttgaccgtc gggttcgatc cctccggata
     4621 gcgcaccccg agctcgtcgc aaagggtttc cagcagctcc ttgaccgtca gacgcgggtt
     4681 gagaatgaac gcgacgtcta gatccggggg cgtctgcttg atcaggttgc gcagcagcgt
     4741 cgtcttgccg gttcccacct cgcccgtgat cagcacgaag ccgccgtgcg agaagccgta
     4801 catcaggtgg gcgagcgcct cgctgtggcc cgcgctcaga tacagaaaat tgggatccgg
     4861 cgcgatggaa aagggcgcct ggttgagctg aaagtaatcc tggtacatca cagcgcgacc
     4921 gtctgggccc ccaggcgcgc gtccttttcg cgccacaagg cctctatcca gggcgaaacg
     4981 acctcacgct gctcgtggtc gttcccgggc gccctcacgt cctgtgggat ctcccggcat
     5041 tgcaccagca ggtgcgctgc cgggcacttg ccctgcatga actcccagaa cgtgggggtt
     5101 cctcgggggt aggtgatggt gacgtcgacg agcctgtcca gctccttgct caggccgctc
     5161 aggacgtagg acaacccgcc gattttcggg ttgaggaggt gcttgaagcg agcgtcctgc
     5221 cgggcctgtt tggcgggtgt gaagcgcgtg ccctccagga aattcagaac cgtggtgggg
     5281 tggttgcgga actcctgaca tgcggccagg gtggcgtcgc gatctgcgcg cacgagctct
     5341 ggcttcttcg caatctgttc cctcgtcatg cgccgcacga acggaaatcc gagcagccgc
     5401 atggccagac cgatgaacgg gatccagatg agctcgcgtt tggtgaagaa cttgatgggt
     5461 ggtatgtgtc catacagtga tgtctgcagc aggagtatgt cggtccagct ctgatggttg
     5521 ctgatcacca gataccacct gtcgcggtcg agccgttcgg cgccctgcca ttgcacctcg
     5581 acgtcgcaca ggcccagcca gccgaagcag gcgcgattgg tagcgaccca gccgtcgatg
     5641 atccagcgat cgagcttccg gctgagccaa cgtttgatgg ccgccgggct cagtgcacgc
     5701 acggctgaaa ccaggaacag cgggatgcac agcacgatgg tgttcaacgc caccagcgtc
     5761 gttgccagta tgcctttcag gaacgccacg aaaccctctt atcatacgtg ctgacctagc
     5821 gctactgcct gggggatccc ggcttgagaa tgcagacgac cacagcaggc gtacggccgc
     5881 atatgacgat cgggcgcctg ctggcggcgg ccgcggccgc ctcgtttctc tccggctgcg
     5941 cgagcctcgg ctactacacc caggccatga gcggtcagct gcagatctcg tcttccgggc
     6001 agcccgtggg cgaattgctt gcccgtctcg aagccgttga ggaccccgat gcgcttcagg
     6061 ccggtctcct ctcgggcctg cgctccagcc agtcggtgct cgctttcgca gaagccgaac
     6121 tcgggctcga agcaggcggt agctactcct cctacgtcga gctggatcgg ccttacgtgg
     6181 tctggaacgt tttcgcggct ccggagctca gcctcgaggc gcggtcctgg tgttatctcg
     6241 tcgtcggctg cgctccgtac cgtggctact tcgagcaggg ccgtgccgag cggtttgcgg
     6301 caggtctcaa ggacaggggc ctggacgtct acgtgggggg cgtgcccgcg tactccacgc
     6361 tcggctggtt cgaagacccg ctgctgagca cgttcattcg ctggccccaa ggaaggctgg
     6421 ccgagttgct gattcacgag ctggctcact ccagggtctg ggtggcgggt gacgtggggt
     6481 tcaacgaagc gtttgcgagc ttcgtgggcc gagagggcgc ccggagctgg atcgagcagc
     6541 gccatggggc ccaagcggcc gatgagtttc tcgagcgcgg caggagctgg ccggcactga
     6601 tgcggcttct gctcgagatc cggcgggctc tggaggagct ttacatcagc ggtcttagcg
     6661 aaggggaaaa acgggcgggc aagtcgaagg tgatcgatcg tgggaaagcc tgttaccaga
     6721 ggaacatttc ccgatacgga tccg
//
LOCUS       DBNHOX010000297         7310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1117439, whole genome shotgun sequence.
ACCESSION   DBNHOX010000297 DBNHOX010000000
VERSION     DBNHOX010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1117439"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2182)
                     /locus_tag="AC16UZ_06495"
     CDS             complement(<1..2182)
                     /locus_tag="AC16UZ_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246370.1"
                     /translation="MVTLLLLSGFLTACGGGGGSRSSESSTQTPQTSEPPPSPALSLA
                     ASKSVVVAGSAVTLSWSSEDADQCNASGDWSGTKSLSGSESVGPVNDDVTFVLDCSGE
                     GGAVEKAVSVNVAPPTLALDASESVVTMGAAVTLTWSSTGATSCEASGGWSGTKDASG
                     SETVGPVEADSEYALSCTGPGGGIDASASVTVAPPTLELNQSESLVMKNAVVTLTWST
                     TSATECEASGDWTGTKAANGSETVGPVQTDSQYLLNCTGPGGMIEETGLVSVAVPPKV
                     ELNASSTFVDPGSPVTLSWNAANADACSASGDWTGQRAVSGSEVVGPLEDTERFSISC
                     TGPFGTSSADVSVVVTGKTLVWQAPSENLDGTPLQDLAGFKVHWGERSRQYTGSRLID
                     SPTTTSWEPDIEPGTYYFAVTAIDEDGTSSWYSNEVVKGVRSAGIPEISLELSDTVVD
                     AGAFVTLSWTARQVESCDASGAWVGAREAAGVEVVGPLVADQDFTLTCTGPAGTVTRR
                     VEVDVTMEEGSPALAYAAVPGVALSLEASRHLVAEGDRVSVSWVASNADACTASGGWA
                     GERPPAGAFESEPLAASTVFSLSCSGPGGNVVREVSVDVVPAPSASLTVSETLVVAGE
                     STTLTWSSENAASCEAFGDWSGVRSVSGSADTGPLLEDSSFGLICSGPLDRAYASVEV
                     RVDRAARAHWTFDEGAGDVLADSRGSSDGQIVGAVWAQGVSGGAL"
     gene            2607..3362
                     /locus_tag="AC16UZ_06500"
     CDS             2607..3362
                     /locus_tag="AC16UZ_06500"
                     /inference="COORDINATES: protein motif:HMM:NF013183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR6246371.1"
                     /translation="MSNRTVTACVLIIGNEILSGRTQDTNLNHLATTLGGWGIQVREA
                     RVIPDIESVIVDAVNTARAAFDYVFTTGGIGPTHDDITAESIAKAFGVGLVTSPEIAA
                     IIEQRPAPPDVMAARMRMARIPDGAKLVDNLTGGPQGFCMDNVYVMAGIPLVMQAMLK
                     SLEGKLRCGPVVRSVSVTAYLGESQIATELGEIQARHPDVDIGSYPFFRNDRYGTSLV
                     IRGTDERELEDVLAKVEAAIVSEGETPQDVHRA"
     gene            3478..4194
                     /locus_tag="AC16UZ_06505"
     CDS             3478..4194
                     /locus_tag="AC16UZ_06505"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246372.1"
                     /translation="MNPSGKKAVVLGGTSGIGLATARQLMEAGAHVVVVSRSDSNAAG
                     ARETLGDSADIRQLDVLDREGLARLFEQLAPFDILVNAATGGERATGPFLQMDLDGFQ
                     GSFRKLWGYVNSVRLGAEHMAEDGCITLVSGSPARKCPPGFAAISSVGNAVEGFVRAV
                     AAEIVPRRINAVSPGFIDTPMFPVEGEAREKFLAQATAGNLIKRPGTAEEVAQAILFT
                     IRNDFVTGTTVDVDGGALLP"
     gene            4191..5213
                     /locus_tag="AC16UZ_06510"
     CDS             4191..5213
                     /locus_tag="AC16UZ_06510"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008126 - acetylesterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HIR6246373.1"
                     /translation="MRFAGEQPFRPPRLLSNGHVQTVLGNRVRQSWVERKAQPMKATS
                     DALVLCTSEGVRLQALASLRDGAPTVVIIHGWLGHADSSYVLSAAGELWAQGFSVIRL
                     NLRDHGGTAHLNEEMFHSARTREVVEAVTQLSTDFATGPVGVLGYSLGGNFALRVARA
                     TGLPTLAVCPAVDPDRTMQAIDQGWMGYRLYFVRKWQQALASKQQAFPARYDFTRALE
                     LRSVSALTDHFVRDHTDYTSTRAYFDRYTLTGPALSGTEATVVYAADDPVIPVAGFAD
                     LAPGIELVPVARGGHCAFLDHPRRASWIDRYAADHFARRLGPAQITPDAPSSARRSGK
                     RPSSSP"
     gene            complement(5147..6334)
                     /locus_tag="AC16UZ_06515"
     CDS             complement(5147..6334)
                     /locus_tag="AC16UZ_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6246374.1"
                     /translation="MSSEGPLHGIRVVELGQLIAGPFCGQLLGDFGAEVIKVEPPGAG
                     DPMRGWGQGAPVWWPVIARNKKSVTLNLREAEGQELLKQLAVKADVLIENFRPGTMEK
                     WGLDYGTLAALNPGLIMVRVSGFGQTGPYAHRAGYGSIGEAMGGIRYLAGDPDRPPSR
                     VGLSIGDSLAATFACLGTLAALHSRHATGRGQVVDSAIYEAVLAMMESTIPEFTEAGV
                     IRERSGAILPKIAPSNVYPTADGEMILIGANQDSVFARLCRAMERLELTEDARYRDHA
                     ARGEHQQELDELIARWTRTLAADALLDVLESNGVPAGRIYRAPEMLADPQYAARESIV
                     AVAHRAFPNLKMQNVAPKLSETPGRIRWHGPELGEHNEEVYGELLGLLPERRAELGAS
                     GVI"
     gene            complement(6324..7259)
                     /locus_tag="AC16UZ_06520"
     CDS             complement(6324..7259)
                     /locus_tag="AC16UZ_06520"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HIR6246375.1"
                     /translation="MSEADQRAVTVMEVSPRDGLQNEAEVLSTQNKLALIDLALEAGC
                     RRLEAVSFAHPARVPQMADAEAVCLGLPQRGDVIYTGLVMNQRGYERLLATERLDEAG
                     LVVPATDTFGQRNQGMDVAANVAMARSVLRDARLRGLRAQATIAVAFGCPFEGEVPVQ
                     RVVAIASAIAEEGPVEIGLADTVGVGVPGQVLEAFSAVAEAVGPNIPLRAHFHDTRNT
                     GIANAFAALQAGVATLDASIGGIGGCPFAPNATGNIATEDLVYMLDRMGVDHGLSLER
                     LLAGAEWLQGVLGRPVPSSLLRAGGFPQPEPVDVV"
BASE COUNT         1284 a         2553 c         2315 g         1158 t
ORIGIN      
        1 caagggcacc gccgctaacc ccctgcgccc agacggcgcc aacgatctgc ccgtcgctgc
       61 tgcccctgga atcggcgagc acgtcacccg cgccttcgtc gaacgtccag tgggcccgag
      121 cggcccggtc aacgcggacc tccacgctgg cataggcgcg gtcaagaggc ccggagcaga
      181 tcaggccaaa gcttgagtct tcgagcagcg ggcccgtgtc cgccgatccg gatacggagc
      241 gtacccccga ccagtcgccg aacgcttcgc agcttgcagc attttccgac gaccacgtca
      301 gggtggtgga ctccccggcg acgaccaggg tttcagagac ggtcagcgaa gcggacggag
      361 ccggaaccac atccaccgag acttcccgca cgacgttgcc ccctggtccc gagcagctca
      421 gggagaagac ggtcgacgcc gcaagcggct cggactcgaa agcgccggcc ggcggtcgtt
      481 cacccgccca ccctcctgac gccgtgcagg catcggcatt ggatgccacc caggagacgc
      541 tgacccgatc accctccgcg acgagatggc gggacgcctc cagagacagg gcgacgccgg
      601 ggaccgccgc gtacgccagg gccggtgagc cttcttccat ggtgacgtcc acctcgacgc
      661 gacgggtcac ggtgccggcc ggccccgtgc aggtcagggt aaagtcctga tcggcgacca
      721 ggggtcccac gacctctacg cccgccgcct cgcgggcacc gacccaggcg cccgacgcgt
      781 cacaagactc cacctgccgg gccgtccagc tgagcgtgac gaacgcaccc gcatcgacga
      841 cggtatcgga aagctcgaga gagatttccg gaattcccgc ggagcggacg cccttgacca
      901 cctcgttgga ataccagctc gaagtaccgt cctcatcgat cgcggtgacc gcgaaatagt
      961 aggtgcccgg ctcgatgtca ggctcccagg aggtcgtggt cggcgagtcg atcagccgag
     1021 aacccgtgta ctgccggctg cgttcccccc agtgaacttt gaagcctgcc aggtcctgaa
     1081 ggggggtgcc gtcgaggttt tccgaaggcg cctgccatac gagcgttttg cccgtcacca
     1141 ccacgcttac gtccgcgctg gacgtgccaa agggcccggt gcaggaaatt gaaaatcgtt
     1201 cagtgtcttc cagcggtccc accacctcgc tgcccgagac ggcgcgctgc ccagtccagt
     1261 cacccgacgc ggagcaggcg tctgcattcg cggcgttcca gctgagggtg accgggctgc
     1321 ccggatcgac gaacgtgctc gatgcgttga gctccacctt cggcggcacg gctacgctca
     1381 cgagccctgt ttcctcgatc atcccgccgg ggcccgtgca gttgagcagg tactgcgaat
     1441 ccgtctgcac ggggcccacc gtctctgaac cattcgccgc cttggtcccc gtccagtcac
     1501 ccgacgcctc gcactcggtg gcggacgtgg tcgaccacgt caacgtcacc accgcgttct
     1561 tcatgacgag actttccgac tggttcagct ccagcgtcgg cggcgccacc gtcaccgaag
     1621 ccgatgcatc gataccaccc ccgggacccg tgcagctcag cgcgtactcc gagtccgcct
     1681 caaccggacc caccgtctcg gatccggacg cgtccttggt ccccgaccag ccacccgacg
     1741 cctcacagct cgtcgccccc gtgctgctcc aggtcagggt taccgcggcg cccatcgtca
     1801 ccaccgactc cgatgcatcc agcgccagcg tcggcggcgc gacattgacg gacaccgcct
     1861 tctcgacggc accgccctcg ccgctgcagt ccaggacgaa ggtgacgtcg tcgttgaccg
     1921 gacccaccga ctcgctgccc gagaggctct tggtgccgga ccagtcgccg gaagcgttgc
     1981 actgatcggc gtcttcagac gaccaggaca gcgtcacggc gctgcccgcc accaccactg
     2041 acttggatgc cgccagagaa agggcggggg aaggcggcgg ttcgctggtc tggggcgtct
     2101 gggtgctgct ttcggacgaa cgggaacccc ctcccccacc acaggcggtg aggaagcccg
     2161 acagcaaaag caatgtgacc atccgcatcg aaatcatggg atggctagac cgatcgcatc
     2221 cagtgccccc ttggatgtga gccaaatgtg tcccgaggta atcagtatat gtggaccagc
     2281 acaggaattc ggcccttccg acacgcccga gccgtcatct atgtaaatga taagtaagga
     2341 aaaagtttgc tctcagcgtg tcgctcccca ggtagacgct cggggattca caactaattt
     2401 caatccattc aaggggttat atatgtcata taggctgaca ttaaaactat ctcatatcga
     2461 ctttacaagc gggcccttcc ctcggtgccc cgccgctcga ggcaacctgt ctgaccaaag
     2521 ggcggcggac tgccggaaag tgttaggatg cgctccgccc gggcggggtg cccggagaac
     2581 cgaacagctg aaacggaacc cctcccatga gcaaccgtac ggtcacggcc tgtgtgctga
     2641 tcatcggcaa cgagatcctc tccggacgca ctcaggacac caatctgaat cacctggcaa
     2701 cgaccttggg gggttggggc attcaggtac gcgaggcccg cgtcattccg gacatcgagt
     2761 cggtcatcgt ggatgccgtg aacacggcgc gagcggcctt cgactacgtt ttcaccaccg
     2821 gcggcatcgg gcccacccac gacgacatca cggccgaatc catcgccaag gcattcggcg
     2881 tgggcctcgt cacgtcaccg gagatcgccg ccatcatcga gcagcgcccc gcgccgccgg
     2941 acgtgatggc cgcccggatg cgcatggcac gcatccccga cggcgccaag ctggtggaca
     3001 acctgaccgg cgggccccag gggttctgca tggacaacgt ctacgtcatg gccggcatac
     3061 ccctggtcat gcaggcgatg ctgaagagcc tggaaggcaa gctgcgctgc gggcccgtgg
     3121 tgcgctccgt ctccgtgacc gcctatctgg gcgagagcca gatcgccacg gagctgggcg
     3181 agattcaggc gcgccacccc gacgtggaca tcggcagcta cccgttcttc cgaaacgacc
     3241 ggtacggcac ctcgctggtg atccgcggaa ccgacgagcg tgagctggag gacgttctcg
     3301 cgaaggtcga ggccgccatc gtctcggaag gcgagacgcc tcaggacgtg caccgggctt
     3361 gagcccgccc gctccttccg cgccaggacg cctgcggcca ggtgccggcg ccagagcgac
     3421 ttcgtccgcg gtatcgcgcg cgaaaacagg attagaaaca caaggagaca gttgaagatg
     3481 aacccgtcag gaaagaaagc cgtcgtgctc ggcggcacgt cgggcatcgg gctcgcgaca
     3541 gctcgccagc tgatggaagc cggcgcccac gtggtggtcg tcagccgatc tgacagcaac
     3601 gccgccggcg cccgggaaac cctgggggac tcggccgaca tccgtcagct ggacgtcctg
     3661 gatcgggagg gcctggcccg gctgttcgag caactcgcgc cgttcgacat tctcgtcaac
     3721 gcggcaaccg gcggcgaacg ggccacgggc ccgtttctgc agatggacct ggacgggttc
     3781 cagggctcct tccgcaagct gtggggctac gtcaactccg ttcggctcgg cgccgagcac
     3841 atggccgagg acggctgcat taccctggtg agcggctcgc cggcacgaaa gtgtccgccc
     3901 ggctttgcgg cgatatccag cgtcggcaac gcggtagagg ggttcgtacg agccgtcgcc
     3961 gccgagatcg ttccgcgacg gatcaacgcg gtgtcacccg gcttcatcga cacgcccatg
     4021 tttccggtag aaggcgaggc gcgcgagaaa ttcctcgctc aggcgaccgc cggcaacctg
     4081 atcaaacggc cgggcaccgc agaagaggtc gcccaggcca tcctcttcac catccgcaac
     4141 gacttcgtga cgggcacgac ggtggacgtc gacggaggcg ccctgctgcc gtgagattcg
     4201 ccggggagca accatttcga ccgccccgcc tgctgagcaa cgggcacgtc cagaccgttc
     4261 tcggcaaccg ggtgcgacag tcctgggtcg agcgtaaggc gcagccgatg aaggcgacca
     4321 gcgatgcact cgtgctctgc acgtccgagg gcgttcggct ccaggccctt gccagcttgc
     4381 gagatggcgc gcccacggtg gtcatcattc acggctggct gggtcacgcc gattcgagct
     4441 acgtcctttc cgccgccggc gagctctggg cacagggttt ttcggtgatc cggctgaacc
     4501 tgcgcgatca cggcgggacg gctcatctga acgaggagat gtttcattcc gcacgaaccc
     4561 gcgaggtcgt ggaagcggta acacagctga gcaccgactt cgcaaccggc cccgtcggcg
     4621 tgctgggtta ttccctgggc ggcaacttcg cgctgcgggt ggcgcgcgcc accggtctgc
     4681 cgacccttgc agtctgtccg gccgtggacc cggatcggac catgcaagcc atcgatcagg
     4741 gctggatggg ctaccgcctg tacttcgtcc gcaagtggca acaggcgctg gcttccaagc
     4801 agcaggcatt ccccgcacgc tacgacttca cccgcgctct ggagctgagg tccgtcagcg
     4861 cgctgaccga tcacttcgta cgcgaccata ccgactacac gagcacgcgc gcctacttcg
     4921 accgctacac gctgaccggg ccggcgctgt ccggcaccga ggcaaccgtc gtctacgcgg
     4981 cggacgatcc cgtcatcccg gtcgcgggct tcgccgatct ggcgccggga atcgaactcg
     5041 tgccggtggc gaggggcggt cactgcgcct tcctcgacca ccccaggcgc gccagctgga
     5101 tcgaccgcta cgcggccgat cacttcgccc gacgactcgg cccggctcag atcacccctg
     5161 acgcgccgag ctccgcacgc cgttccggta aaaggcccag cagctcgccg tagacctctt
     5221 cgttgtgctc gccgagctcc gggccgtgcc agcggatacg gccaggcgtt tccgagagct
     5281 tcggcgcgac gttctgcatc ttgagattgg gaaatgcccg gtgcgctacg gccacgatgg
     5341 attcccgcgc cgcgtactga ggatccgcga gcatctccgg cgcccggtag attctcccgg
     5401 ccggcacgcc gttcgactcc agaacgtcga gcaacgcatc cgccgccagc gtccgggtcc
     5461 agcgggcaat gagctcgtcg agctcctgct ggtgctcgcc ccgggccgcg tggtctcgat
     5521 agcgcgcgtc ttccgtaagc tcaagccgct ccatggcgcg gcaaaggcgg gcgaacacgg
     5581 aatcctggtt ggccccgatg aggatcatct ccccgtcggc agtggggtag acgttggaag
     5641 gcgcgatctt cggaaggatg gcgccgctgc gctcacggat gacgccggct tcggtgaact
     5701 cgggaatcgt cgactccatc atggccagca cggcttcgta gatggccgaa tccaccacct
     5761 gccctcgtcc ggtcgcatgc cggctgtgga gggccgccag ggtgcccagg caggcgaagg
     5821 tggctgccag ggagtcgccg atggaaagcc ccacgcggct cggcggccgg tccgggtcac
     5881 ccgccagata gcgtataccg cccatggcct cgccgatcga gccgtagccc gcccggtgag
     5941 cgtatgggcc cgtctgtccg aatcccgaca ccctgaccat gatgaggccc ggattcagcg
     6001 cggcaagagt gccgtagtca aggccccact tctccatggt gccgggccgg aagttctcga
     6061 tcagcacgtc cgccttgacc gcgagctgct tgagcagctc ctgcccttct gcctcgcgca
     6121 ggttcagcgt caccgatttc ttgttgcgcg cgataaccgg ccaccagacg ggcgcgccct
     6181 ggccccaccc gcgcatggga tcccccgcac cgggaggttc caccttgatc acttccgcac
     6241 cgaagtcacc cagcagctga ccgcagaaag gcccggcaat cagctggccc agctcgacga
     6301 cgcgtatgcc gtgcaggggc ccttcagacg acatcgacgg gctcgggctg cggaaaaccg
     6361 ccggcacgca gaaggctcga cggcaccgga cgaccaagca ccccctgcaa ccattccgcc
     6421 ccggccagca ggcgttcgag ggacaacccg tggtctacgc ccatgcgatc gagcatgtaa
     6481 acgagatcct cggtggcgat gttgccggtg gcgttggggg cgaaggggca accgccgata
     6541 ccgccgatgc tggcgtccag ggtggcaacc cccgcctgca gggcggcgaa ggcgttggca
     6601 atgccggtgt tacgggtgtc gtgaaagtgg gccctcaacg ggatgttcgg gccgacggct
     6661 tctgccaccg cgctgaacgc ctcgagcacc tgaccgggaa cgccgacccc caccgtgtcg
     6721 gccaggccga tctccaccgg tccctcttcg gcaatggccg acgcgatcgc cacgacgcgc
     6781 tggacgggca cctcgccctc gaaggggcaa ccgaacgcca cggcaatggt cgcctgggcg
     6841 cgcagccctc tcaaacgcgc gtcccgcagc accgaacggg ccatggcgac gttggcggca
     6901 acgtccatgc cctgattgcg ctggccgaac gtgtcggtgg ccggaacgac cagcccggcc
     6961 tcgtcgagac gctcagtggc caaaagacgc tcgtagccgc gctggttcat cacgagaccg
     7021 gtgtagatca cgtctccccg ctgaggcagg cccaggcaca cggcttccgc atcggccatc
     7081 tgtggaaccc gagcagggtg ggcaaagctg accgcctcca gccggcgaca gcccgcctcg
     7141 agggccaggt cgatgagggc cagcttgttc tgcgtgctga gcacctcggc ttcgttctgc
     7201 agcccgtcgc gggggctcac ctccatgacg gtcacggccc gctggtcggc ttctgacatt
     7261 tacgtgacct cctggttatg ccctgaacgg accaccgcga cggcctaagg
//
LOCUS       DBNHOX010000298         3280 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_345929, whole genome shotgun sequence.
ACCESSION   DBNHOX010000298 DBNHOX010000000
VERSION     DBNHOX010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_345929"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..771
                     /locus_tag="AC16UZ_06525"
     CDS             76..771
                     /locus_tag="AC16UZ_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HIR6246376.1"
                     /translation="MSSRKKSDKKVTRVRMLIVGFTILVGIVVLGYGTLYSTGLGEGE
                     FLAGEHYRVIENPPRRRAGAPIMVREYFSYGCIHCRNFEPLLDEWRATQPERTAFERS
                     PVAFSPAWQLLAQTYFTLKRLDVLEQNHMRLFRAIHDNARQFATPESMADFVDGNGVT
                     RAEFLRMFNSPEVRGDLREADAIQRNIVISSVPTLVVDDRYVVNMDLGRKRALDVVDY
                     LISQQLAETAAAP"
     gene            complement(798..2894)
                     /locus_tag="AC16UZ_06530"
     CDS             complement(798..2894)
                     /locus_tag="AC16UZ_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6246377.1"
                     /translation="MNINTRGLLAGGLLLLAPVVSAQADEFVLIEEIVVTSEFRPTAL
                     EDTAASVSVISLADQKAGALNHLEEVVGWAPNVNLASGGGRARFVQIRGIGERGQFAE
                     PLNSSVGLLLDGVDMSGVGTVATLFDVEQVEILRGPQGTLYGANALAGLINVVSADPS
                     DELYTRLSLDAGNYGAFGVGGVISGPLGDNVGYRLAVQSYGDDGFMDNRFLDRDDTDD
                     HDELTIRGKLHWQVGPETDLRLTLGHVDVDNGYDAFSLDNDRNTLSDAPGQDRQQSTF
                     GSAQLNTALGEAVTLQATVGYADSDIDYGYDEDWTYAGFDPIGYTSEDRYRRDRQTLN
                     FDVRLLSGQAARMPGSVDWVLGVYGLHQEVDLERDYTFFFGEPFASRFEVDRLAVYGE
                     LGVQMSQRIRASLGLRFEHHESDYSDSEAVAADPKDDMVGGRLVLEADVADDAMVYFS
                     ASRGYKAGGFNTSGTLERELRDFDPEKLWNYEVGVKGTSADGRLQARVAAFLMKRDDV
                     QVSTFIAVPREDGSTEFIDLIDNAAEGTNAGLEAEVLWMPTEQLEVFASFGWLDTEFD
                     DFINAAGEDLDGEEQAHAPGYQFHVWGEYRFADGWFLRLAVEGRDAFYFTDGRRFAPK
                     AEDLKSDSYELVHASAGYEADRWRVSAWGRNLTDEDYAVRGFYFGNDPRDFYTARGFT
                     QLGEPRRYGITLQLEL"
     regulatory      complement(2946..3057)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          599 a         1075 c         1032 g          574 t
ORIGIN      
        1 aacgggcgcg ctacccgagt ttccacacca gcgtcgctgt gggtaaactc cggcgcaaac
       61 gacaggggag gccccatgtc cagtcgcaaa aagtcggaca agaaagtgac ccgggtgcgc
      121 atgctgatcg tcggcttcac catcctggtc ggcatcgtgg tgctggggta cggcacgctc
      181 tacagcaccg gcctgggcga aggcgagttt ctggccggcg agcattaccg ggtcatcgag
      241 aacccgccca gacggcgggc gggcgcgccc atcatggtgc gcgagtattt ctcctacggc
      301 tgcatccact gcaggaattt cgagccgctg ctcgacgagt ggcgagcgac gcagccggaa
      361 cggaccgcct tcgagcgctc accagtggct ttctcgccgg cctggcagct gctggcgcag
      421 acctatttca cgttgaagcg cctggacgtg ctggagcaga accacatgcg tctgttccga
      481 gccattcacg ataacgctcg gcagttcgca acgccggaat ccatggccga tttcgtagac
      541 ggcaatggcg ttacccgagc cgaatttctc cggatgttca actcaccgga agtgcgcggc
      601 gacctgcgcg aagcagacgc catccagcgc aacatcgtca tctccagcgt ccccaccctg
      661 gtcgtggacg acaggtacgt ggtgaacatg gacctggggc gcaaaagagc gctcgacgtg
      721 gtcgactatc tgatctccca gcagcttgca gagactgccg ccgcgccctg acgcggcctg
      781 cacgggattg acgacggtca gagctccagc tggagggtga ttccgtaacg gcgaggctcg
      841 ccgagctggg tgaagccgcg cgccgtgtag aaatcccgcg gatcgttgcc gaaatagaag
      901 cctcgtacgg cgtagtcctc gtccgtcaga ttgcggcccc aggcggacac ccgccaccgg
      961 tctgcttcgt aacccgcaga ggcatgcacc agctcgtagc tgtccgactt cagatcttcg
     1021 gccttgggcg cgaaccggcg gccgtcggtg aagtaaaaag cgtccctgcc ctccaccgcc
     1081 aggcgcagga accagccgtc cgcgaatcga tactcgcccc agacgtgaaa ctggtagccg
     1141 ggcgcgtgag cctgctcctc gccatcgaga tcctctcccg cggcgttgat gaaatcgtcg
     1201 aactcggtat cgagccagcc gaagctggca aacacctcta gctgctcggt gggcatccag
     1261 aggacctcgg cttccagccc ggcgttggtg ccttcggccg cgttgtcgat gaggtcgatg
     1321 aactcggtgc tgccgtcttc gcggggcacg gcgatgaaag tggacacctg aacgtcgtcc
     1381 cttttcatca gaaacgccgc gacgcgcgct tggagccggc cgtcggccga cgtccccttc
     1441 acgcccacct cgtagttcca gagcttttcg ggatcgaaat cccgcagctc gcgttccagg
     1501 gtgccgctgg tattgaagcc gccggccttg tagccgcggc tggcgctgaa atagaccatg
     1561 gcgtcgtccg cgacgtccgc ttccagcacc aaccgaccgc cgaccatgtc gtccttcgga
     1621 tcggccgcca ccgcctcgga atccgagtag tcggactcgt ggtgctcgaa ccgaagcccc
     1681 aaagaggccc ggatgcgctg cgacatctgc acgccgagct cgccgtagac ggccagccgg
     1741 tccacctcga aacggctggc gaacggctcg ccgaagaaga aggtgtagtc gcgctcgagg
     1801 tccacttcct gatgcaggcc gtacacgccc agcacccagt cgacggaccc gggcattcgg
     1861 gctgcctgcc cggaaagcag ccggacatcg aagttgagcg tctggcgatc gcgccggtag
     1921 cggtcttcgg aggtgtagcc gatggggtcg aaacccgcgt aggtccagtc ctcgtcgtaa
     1981 ccgtagtcga tgtcgctgtc cgcatagccc acggtagcct gtagggtgac ggcctcgccc
     2041 agcgcggtgt tcagctgagc gctgccgaac gtgctctgct ggcggtcctg gcccggggca
     2101 tcggaaagcg tgttgcggtc gttgtcgagc gagaacgcat cgtagccgtt gtccacgtcc
     2161 acgtggccca gcgtgagtcg cagatcggtt tccgggccaa cctgccaatg gagcttgccg
     2221 cggatggtca gctcgtcgtg gtcatccgtg tcgtcccgat ccagaaagcg gttgtccatg
     2281 aagccatcgt caccgtagct ctgcacggcc aggcgatagc cgacgttgtc ccccagggga
     2341 ccggagatca cgccacccac cccgaacgcc ccgtagttgc cggcatccag ggagagccgc
     2401 gtgtagagct cgtcgctggg gtcggcgctg accacgttga tgaggccagc cagggcattc
     2461 gccccgtaga gcgtcccctg gggcccgcgc agaatttcca cctgttccac atcgaagagc
     2521 gtggcgacgg tgccgacccc gctcatgtct acgccgtcga gcagaagccc taccgacgag
     2581 ttgagcggct cggcgaactg gcccctctca ccgatgccgc ggatctgcac gaatcgggcc
     2641 cggccgccgc cgctggccag attcacgttg ggcgcccacc ccacaacctc ctcgagatgg
     2701 ttgagagccc ctgctttctg gtcggccagg gagatcaccg acacgctcgc cgcggtgtcc
     2761 tccagcgccg tggggcggaa ctcgctggtt acgacgatct cctcgatgag aacgaactcg
     2821 tcggcctgtg ccgaaaccac cggtgccagc aacagcaagc cgccagccag cagtcctcgg
     2881 gtattgatgt tcatggtcca cccactcagg ttgccttgtt gcgaccgcga aggggtgaaa
     2941 tgacggagaa gagaagtgcc tattcctacg ccggaattaa ccgaatcagg ttcaaagggt
     3001 ccaccgacga gccgcgctcg accggtgtct caggcagtac cgccccttag gtctgcagag
     3061 attctcgccc ggggtccttg cgggcgcaag cgcgtgcttc agttagggtt cagattcgcc
     3121 acgtagagtg acgctgagaa accgcagatc cgaagcgaaa tggaagaata tcaagcagtt
     3181 gtgcagacca tcgccctgac catgggcgtg gcgtgggcga gcgggctgaa cctctacgcg
     3241 gccttggccg tgctcggcct taccggatca ctgggctacg
//
LOCUS       DBNHOX010000299         3491 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1631724, whole genome shotgun sequence.
ACCESSION   DBNHOX010000299 DBNHOX010000000
VERSION     DBNHOX010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1631724"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="AC16UZ_06535"
     CDS             <1..433
                     /locus_tag="AC16UZ_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR6246378.1"
                     /translation="KTVVDQQFEIGRRIVAAGFVPIIEPEVDIHCPDKAGAEQILKGA
                     LLAGLDALRPEERVMFKLTLPEADNFYAECIDHPNLVRMVALSGGYSREEANARLARN
                     NRMVASFSRALTEGLSAQQTQAEFDAALDASIQSIFEASLT"
     gene            483..1565
                     /gene="hppD"
                     /locus_tag="AC16UZ_06540"
     CDS             483..1565
                     /gene="hppD"
                     /locus_tag="AC16UZ_06540"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HIR6246379.1"
                     /translation="MTNRVDNPMGLDGFEFVEFSATQPGQIEPVFAMLGFQEVARHRS
                     KDVRLWRQGGINLVVNYEPRSVAWYYAEEHGPSACGLAFRVRDAHVAYKRALELGAQP
                     VEIPTGPMELRLPAIRGIGGAPLYLIDRYRPGSSIYDIDFEYLDAVDRFPEGIGLEII
                     DHLTHNVYRGRMDHWARFYETLFNFQEIRHFDIRGEQTGLSSRAMTAPDGKIRIPLNE
                     EARGAGDGQVEEFLLQYNGEGIQHIAFACQDLPSKLDRLAAAGVPLMEPPPDAYYDML
                     EDRLPGHGEPVGELKQRGILLDGSTGEGEPRLLLQIFSEPLIGPIFFEFIERKRDEGF
                     GEGNFQALFESMERDQIRRGAIEGPS"
     gene            1562..2432
                     /locus_tag="AC16UZ_06545"
                     /pseudo
     CDS             1562..2432
                     /locus_tag="AC16UZ_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003200383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
     gene            2535..3053
                     /locus_tag="AC16UZ_06550"
     CDS             2535..3053
                     /locus_tag="AC16UZ_06550"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015094380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6246380.1"
                     /translation="MVAEAKPVGPKRFRESYGRYLEDFAVGDVYEHRPGRTITETDNT
                     WFTLLTMNQHPLHFDKEYGANSEFGRVLVNSCLTLSLVTGMSVSDVSQKTVANLGWNN
                     VRLSGPVFVGDTIYAESEVLEIRESKSRPNQGIVTIRSRGIKQDGTEVISFERAMLIP
                     ARGHAVDDAIDY"
     gene            3064..>3491
                     /locus_tag="AC16UZ_06555"
     CDS             3064..>3491
                     /locus_tag="AC16UZ_06555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6246381.1"
                     /translation="MAELSEQDRLVLDSVDRFLDRDVRPVARALEQEDAYPHELVEKM
                     KALGLFGATIDERHGGLGLTASVYALIVERVSAVWMSVSGIFNSHLIMCAAVQRFGTE
                     AMKERLLPAMATGELRGGIALTEPDCGTDLQAIRCRARRDG"
BASE COUNT          640 a         1035 c         1154 g          662 t
ORIGIN      
        1 caagaccgtc gtggatcagc agttcgagat cggtcgccga atcgtcgccg cgggattcgt
       61 gcccatcatc gagcccgagg tggacattca ctgtccggac aaggctggag ccgagcagat
      121 tctcaaaggc gctctcttgg ccggcctgga tgccctgagg cccgaggaga gggtaatgtt
      181 caagctcacc ttgcccgagg cggacaactt ttacgcggag tgcatcgatc atccgaatct
      241 ggtgcggatg gtggcgctct ccggcggcta ctcacgggag gaggccaatg cgcggctcgc
      301 ccgcaacaac cggatggtcg ccagcttctc gcgggcgctg acggagggac tttccgcgca
      361 gcagacccag gcagaattcg acgcggcgct cgatgcctcc attcaaagca tcttcgaggc
      421 ctctctaacc tgaagcgcca gcgcggccag ctgcctgaac ccgtcacggc gatgcgataa
      481 ggatgacgaa ccgcgttgac aatcccatgg gcctcgacgg tttcgagttc gtcgagtttt
      541 ccgcaacgca gccgggtcag atcgagccgg tcttcgcgat gctcgggttt caagaagtgg
      601 cccggcatcg ctccaaggac gtccgcctct ggcggcaggg cgggatcaac ctcgtcgtca
      661 actacgagcc gcgaagcgtt gcctggtact acgccgagga gcacgggcct tcggcatgcg
      721 gtctcgcatt cagggtccgc gatgcccacg ttgcctacaa gcgggcgctg gagctcggcg
      781 cccagcccgt cgagattccc acaggcccga tggagctgcg cctgccggca atccgcggaa
      841 tcggcggcgc accgctgtac ctgatcgatc gctatcggcc gggcagcagc atctacgata
      901 tcgacttcga atatctcgac gcagtggatc ggtttcccga gggtatcggt ctcgaaatca
      961 tcgatcacct tacgcataac gtctaccggg ggcggatgga tcattgggcc cgtttctacg
     1021 aaacgctttt caactttcag gagatccgac atttcgacat tcggggtgag cagacgggct
     1081 tgagctcccg ggccatgacc gccccggacg gcaagatccg gataccgctc aatgaggaag
     1141 cccgtggagc cggcgacggg caggtggaag agtttcttct gcagtacaac ggtgagggaa
     1201 tccagcacat cgcttttgcc tgccaggacc tgccgtcgaa gctggatcgt ctggcagcgg
     1261 ccggggtgcc attgatggag cctccgccgg acgcctacta cgacatgctg gaagatcgcc
     1321 ttccgggtca cggggagccc gtgggcgaac tcaagcagcg tggaatcctg ttggatggga
     1381 gtacggggga gggtgagccg aggctgctgc tgcagatctt ctccgagccg ttgatcggcc
     1441 cgatattttt cgagttcatc gagcgcaaac gcgacgaagg gttcggagaa ggcaacttcc
     1501 aggcgctgtt cgaatccatg gaacgggatc agattcgtcg cggggccatc gaaggaccat
     1561 cgtgaagctt gcaacgcgag gggaggatcg ggatggtgag ctcctcgtcg tctcgcggga
     1621 tctgagccgg gcggttcgcg tgggggaaat cgctcccacg ttgcagggcg ccctcgaccg
     1681 atggggtacg gttgctgaaa agcttgcctc cgtctacgaa cgactcaacg acggtgccct
     1741 ggaagcctcg tttccattcg tccccgcacg ttgcgcggcg ccgctgccgc gcgcttacca
     1801 atgggcggac ggcagtgcct acgtcaatca cgtggagctg gtccgtcgcg cgcggggcgc
     1861 cgacatgccc gcgagcttct ggcgtgaacc tttgatttat cagggcggat ccgacgtcat
     1921 gctggggccc accgatgaca tcgtcgtgag gagcagcgac tgggggctcg atttcgaggc
     1981 cgagatcgcc gtcatcgtcg atgacgtcga gatgggcacc tcgacggtcg atgccggtga
     2041 ccacataaag cttctcatgc tcgtcaatga cgtctcgctg cgcgggctga tacccgggga
     2101 gctggacaaa ggcttcggct tctatcaatc caagccggcc acggcgtttt cgccggtggc
     2161 cgtgtctccc gacgagcttt gcggcgactg gcgtgagaac aaggtgcaac gccccctgtt
     2221 gagctatgtc aacgggcggc tcttcggtcg cggccggcgg cgtgggctac tcgtgcattg
     2281 cagagctgcg catgatcgag accattcgcg atggggcgcc gcagacgtcc ttcctgcagg
     2341 cgggggatcg ggtcaccatc gaaatgctcg accgggacgg ctccagcatc ttcggctcca
     2401 tcgaccagcg ggtggtggtc gggaccggct gaattgtcat tcggggtcgt gaatgtgttc
     2461 tacttttgtc ccgaagcgag gccgggcaaa cgcgcgcttc gggagcgggc gacgcaaccg
     2521 aggaggtggc aaacatggtg gcagaggcaa agccggtggg gcctaagcgg ttccgagagt
     2581 cctacgggcg atatctggaa gatttcgccg tgggcgacgt gtacgaacat cgaccgggac
     2641 ggacgatcac tgaaactgac aacacctggt tcacgctgtt gaccatgaac cagcatccgc
     2701 ttcatttcga caaggagtac ggggcgaatt ccgagttcgg gagggtgctg gtcaactcct
     2761 gcctgacgct gtctctggtg actggtatgt cggtctctga cgtcagccag aagacggtgg
     2821 ccaatctggg ctggaacaac gtcaggctga gcgggccggt gttcgtcggc gacacgattt
     2881 acgccgagtc agaggttttg gagatccggg aatccaaatc ccgcccgaat cagggcatcg
     2941 tgacgatccg ttctcgcggc atcaagcagg acggcacgga ggtcatcagc ttcgagcgcg
     3001 ccatgctcat tccggcccgg ggccacgccg tggacgatgc catcgactat tgaggcttgt
     3061 gtcatggccg aactctcaga acaggaccgg ctcgtgctgg actccgtcga tcggtttctc
     3121 gatcgggacg tacgaccagt ggcgcgcgcg ctggagcagg aagacgccta tcctcacgag
     3181 ctagtggaga agatgaaggc gctcggcctg ttcggcgcca ccatcgacga gcgtcatggt
     3241 gggctgggac tgaccgcatc cgtgtacgcg ctcatcgtcg agcgcgtgtc cgccgtctgg
     3301 atgtctgtgt cgggcatctt caactctcat ctgatcatgt gtgccgccgt gcaacgtttc
     3361 ggcaccgagg cgatgaagga acgccttctc ccggcaatgg ccacgggcga gctgcgaggg
     3421 gggatcgccc tcaccgagcc ggactgtgga accgacctgc aggccattcg ttgccgtgcc
     3481 cgccgcgacg g
//
LOCUS       DBNHOX010000300         9642 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_603815, whole genome shotgun sequence.
ACCESSION   DBNHOX010000300 DBNHOX010000000
VERSION     DBNHOX010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9642)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9642
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_603815"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..688
                     /locus_tag="AC16UZ_06560"
     CDS             125..688
                     /locus_tag="AC16UZ_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246382.1"
                     /translation="MRIEPLKGWHRYTELMNAETDPRRKAMLDNMRHHLKYECLGDPA
                     IFDSMVPDPEYRFYASFDNAVLTGMEEVREFYYNIWNTRSSLVELAIHRCAAADWGVA
                     CEGEWYQQVPGETLIANGQQVEDRDAWYLSHAHLSWFFPFREIDGRMLLEGEICYIDE
                     PGSTLQKLDPADVITLEEAKASWAALP"
     gene            685..1206
                     /locus_tag="AC16UZ_06565"
     CDS             685..1206
                     /locus_tag="AC16UZ_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6246383.1"
                     /translation="MTLPISATFHHVGITVSNLERAVEWYSKVFGLKPGVALEVSGPT
                     GEQVLRLPPHIHRAALLPVGDFAIELLEFEPTRRQMDMRQDDVGYVYICFAVDDLDDV
                     YARLSAEGIDFHTPPMLAEEGQIAGSKFCVMRDPDGKTIELVEPGPGMKVPALHAAAR
                     RGVALDDPLIMDG"
     gene            complement(1234..2412)
                     /locus_tag="AC16UZ_06570"
     CDS             complement(1234..2412)
                     /locus_tag="AC16UZ_06570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase family protein"
                     /protein_id="HIR6246384.1"
                     /translation="MKPQPFRIEVPEETLRDLADRLDRSGWPDDFANDDWSYGTNGAY
                     LRDLVGFWRRDYDWRAVERKINAFDHYRVEIEGMPVHFLREPGRGPNPVPLILSHGWP
                     WTFWDLNEVIRPLADPASFGGDPEDAFEVIVPSLPGFGFSTPLTTPGINFWRTADLWH
                     TLMTEVLGFQRFGAQGGDWGALVTAQLGHKYAESLFGIHLTNLIPLTLFNHQRPWDIT
                     AGVMVPDDVPADERDRQLAQQRRIASHVAVQVLDPQTLAYGLHDSPVGLLAWLLERRY
                     FWGDCNGDVESRFSREHLITTAMIYWVTSSFATSARYYAEAARNPWRPSHDRSPQVEA
                     PAGATYLGGDVGRGTIPAVREAFDLRYENSHPVGGHFAPMEEPEVVINDIRETFRPLR
                     "
     gene            complement(2419..2982)
                     /locus_tag="AC16UZ_06575"
     CDS             complement(2419..2982)
                     /locus_tag="AC16UZ_06575"
                     /inference="COORDINATES: protein motif:HMM:NF025050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6246385.1"
                     /translation="MSNGTMPIFQHAYFVNDIEEAAHRWNRLYGAGPFVLVPHHKTDK
                     FQYRGTDQEADVSYGFGYLGENQIQFIQQHDETPSIYRDMYQAGEEGFHHVGCLVNDF
                     AAARQRMLDLGFVNACELWADDVNAAYFDTRSVNGGFTEIHGDPVHILGTFATWKRAH
                     QNLKPGDSPIMDMSAPFPDGRDPGYLD"
     gene            complement(3025..4062)
                     /locus_tag="AC16UZ_06580"
     CDS             complement(3025..4062)
                     /locus_tag="AC16UZ_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6246386.1"
                     /translation="MKLGILGGNIGRKTGINIEAIRKAESLGYDSVWTAEAWGGDAVT
                     PATWILAQTEKIKVGTAIMQMPARTPAMAAMTAMSLAELSGGRFIIGLGASGPQVVEG
                     WHGVPYGKPMTRLREYVAIMRKIFAREDKLTFDGEMYQIPYTGPGSTGLGKPLKSILH
                     CEEDIRIFAASITPKGVETAAEVADGFFPIWMDPEKFSVFEEPIRKGFAAAGDKDLSS
                     FEVSPFVTVVIGDDVEQCMAPIRGNMALYIGGMGARNKNFYNDYAKALGFEDAAVEIQ
                     DHFLAGRREEAMRAVPAELIDACHLVGPADRIRERLQRWKAAGGNGQVASMLLGTQQP
                     EALELVASEML"
     gene            complement(4097..5773)
                     /locus_tag="AC16UZ_06585"
     CDS             complement(4097..5773)
                     /locus_tag="AC16UZ_06585"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6246387.1"
                     /translation="MLSRSEALEALTAPGQPYELVDVELYGRSCRAFRNAPPLLRDLY
                     ASTASDLPFIVYGGERLSFADAWAESCRIAILLMEQYGVAKGDRVAISMRNYPEWMTA
                     FAAVTSIGAIAVAMNALWTPEEMEYGLKDSGAKVLLADEERLARLALCADPPAGLAVL
                     AVRAADDAGTGARDLREALAGVPAGNMPAVDVAPEDDATILYTSGSTGHPKGAVSCHR
                     SLVTALLSWELDAHAGAYMQGVDLPAPESQPATLLAVPLFHATGSHAVYLASYRSQRR
                     VVSMYKWDVEEAAELIERERISSFVAPPAMTGDLVQFAARTNRDLSCLDRVGGGGAAR
                     APEQVRGIRRTFAKAMPSTGWGMTETNAIGTVISGQEYLDRPESSGRCSAVLELRVVD
                     PEGRELPCGERGELQVRGTSLIRGYWNRPEATAEAIVDGWLRTGDVAYLDDDGYLFIV
                     DRIKDLVIRGGENIGCGAVEAALQEHPDVIEACVYAVPDERLGEEVGATVYICRPVDE
                     EEIRNFLAPRLARFEIPRYLSFSTQPLPRTASGKIFKRQLKQEAADRLTG"
     gene            5911..6699
                     /locus_tag="AC16UZ_06590"
     CDS             5911..6699
                     /locus_tag="AC16UZ_06590"
                     /inference="COORDINATES: protein motif:HMM:NF023358.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HIR6246388.1"
                     /translation="MKPYGKRHASWVWLTLFLLTPNPSRSEAPTATAGDALDQAQSYQ
                     TIAGRSQARVEDLDDAALAMLSQYNGEVDRYEDLRTYNDNLRELLASQRAEKERLNAE
                     LAGIEIVRQEIVPLMVQMIDVLDSFVALDQPLLIDERTSRVESLRRNLTRSDVDLAER
                     YRRIIEAYQIEAEYGQTIEAYEGPIVIDDRELTVDFLRVGRIGLYYLSLDRAEGGIWN
                     PAEKRWTPLAENYLDDLDLAVRMARKQAPPNLMDLPMWTTGGES"
     gene            6696..8048
                     /locus_tag="AC16UZ_06595"
     CDS             6696..8048
                     /locus_tag="AC16UZ_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6246389.1"
                     /translation="MRRTAITLLALLAPASWAAAPTTLDELIDSLKDSSALQAKRLQE
                     REARFVAARNERERLLEDARDRRREAEREADRLRADFEGSEQELADLETRLDENAGDL
                     RELFTVVRQITGDVAPMVESSLVSAEQGPRNALLRRLAEPGAIPAAEELRELWMILLE
                     EMNHSGRVSRFEAPVISADGAERTRRVTRIGTFTALADGSYLRYLPETGRLLALGRQP
                     AGVDVGDVLAFEEAREPLQRVAIDPSRGSILSLIVQTPDVRERIEQGGVIGYIILALG
                     ALGLLLGVERLVTIGVARQRTRRVLADSAAAGDHVIVELRELARDEAYLADAEALSAK
                     MDEVVTIASEKLRRGLPTLAIFAAVSPLLGLLGTVTGMIETFQVITLFGAGDPRLMSG
                     GISQALITTQLGLAVAIPLLLIQSFVQGRANALIATLDETAAELFAQGRTHRGSDHAG
                     "
     gene            8038..8577
                     /locus_tag="AC16UZ_06600"
     CDS             8038..8577
                     /locus_tag="AC16UZ_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6246390.1"
                     /translation="MLGDLFGNSDLIMAGGWIIVLIMALTFVMWMMILDRYVYLWRDR
                     GRAVSRKFALWQQYSSPDPLNNKRLRAALTGQFLEDSRRWIGTIQVITAVLPLLGLLG
                     TVTGMIKTFDVLTAFGTGNIRGMAEGISQALITTMAGLIAALTGIYFANDLEERIATE
                     ADHLAGRFNEGFADRGNGR"
     gene            8574..8984
                     /locus_tag="AC16UZ_06605"
     CDS             8574..8984
                     /locus_tag="AC16UZ_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR6246391.1"
                     /translation="MKLHTREEGGSAAINLTPLIDMVFILLIFFAVNATFVKITGVSV
                     DQPTAKTTSLQPASTILIAVTDAGEIWIDKKKVDKRRVRGVVERMFIEAPDAAVVVLS
                     DEESRTGLVVEVIDQVRLAGAGRVALAAAEPEEQ"
     gene            8984..9628
                     /locus_tag="AC16UZ_06610"
     CDS             8984..9628
                     /locus_tag="AC16UZ_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6246392.1"
                     /translation="MALRQGFIFLGALAVTLSLYVMMDRMVGRDRTRLLSPVDAQSIE
                     FVRTEMEDRSRTKDRRRKPPPKPQEMKRVRADLQTNLNRAADLPTETLAFNIKSLLGE
                     GGGVALGERLVQGSGAALELVMASDLTPLSMLPPQYPPSALMRGVEGWVDMVFIVPEE
                     GGVVDPTILESRPGTTFDAAAKAAAMRWRFRPVVRNGQPTPIRARIRIDFELPE"
BASE COUNT         1684 a         3198 c         3144 g         1616 t
ORIGIN      
        1 tccgttaccg cctgctcccg ggcgtctggt gaggaccatc gcccgacgcc ggcctccggc
       61 aggcagagac gccgacaaat cgtgataact taagcagctc gatcgttaag aatggggaga
      121 cgagatgcgc atcgaaccgc tgaagggctg gcaccgctac acggagctga tgaacgcgga
      181 gaccgacccg cgccgcaagg ccatgctcga caacatgcgg caccacctga aatacgaatg
      241 cctcggcgac cctgcgatct tcgattccat ggttcccgac cccgaatacc gtttctacgc
      301 gagcttcgac aacgccgtgc tcaccggcat ggaagaggtc cgggagttct actacaacat
      361 ctggaacacg cgcagcagcc tggtggagct cgccatccac cggtgcgcgg ccgcggactg
      421 gggcgtggcc tgcgagggcg agtggtacca gcaggtgccg ggtgaaacgc tcatcgccaa
      481 cggccagcag gtggaagatc gtgacgcctg gtatctgtct cacgcgcacc tttcctggtt
      541 ctttccgttc cgtgagatcg acgggcgcat gctgctggaa ggcgagatct gctacatcga
      601 cgagccgggc agcacgctgc agaaactcga tcccgccgac gtgatcacgc tggaagaggc
      661 aaaggcctcc tgggccgcgc tcccatgacc ctgccgatat cagcaacctt tcaccacgtc
      721 ggcatcacgg tgagcaatct ggagcgcgcc gttgagtggt acagcaaggt gttcgggctg
      781 aagcccgggg tagcgctgga agtttcaggc cccaccggcg agcaggtgct gcggcttccg
      841 ccccacatac atcgcgccgc gctgctgccc gtcggcgatt tcgccataga gctgctggag
      901 ttcgaaccga cccgccggca gatggacatg cgccaggatg acgtcggcta tgtttacatc
      961 tgcttcgccg tggacgatct cgacgatgtc tacgcccgtc tcagcgccga gggcatcgac
     1021 tttcacacgc cgcccatgct ggccgaggaa ggtcagatcg ccggctccaa gttctgcgtg
     1081 atgcgcgacc ccgacggcaa gaccatcgag ctggtggagc ccgggcccgg catgaaggtt
     1141 cccgccctgc acgcagccgc ccggcgaggc gtcgccctgg acgacccgct gatcatggac
     1201 ggctagccgt ccccgccatc gaccgcgcga aagtcaccgc aacgggcgga acgtctcccg
     1261 gatgtcgttg atcacgacct ccggctcctc catcggggcg aagtggccgc ccacgggatg
     1321 tgagttctcg tagcgcaggt cgaacgcctc ccgcacggcc ggaatcgttc cacgacccac
     1381 gtcccctccc aggtaggtgg cccccgccgg cgcctccacc tggggcgagc ggtcgtggct
     1441 cggccgccac ggattgcgcg cggcctcggc gtagtatcgg gcagacgtgg cgaagctgga
     1501 cgttacccag tagatcatcg ccgtggtgat caggtgctcg cggctgaagc gggattccac
     1561 gtcgccgttg cagtcgcccc agaagtagcg gcgttccagc agccacgcca gcagccccac
     1621 gggggagtcg tgcagcccgt acgccagcgt ctgcggatcg agcacctgca ccgccacgtg
     1681 gctggcgatg cgccgctgct gagcgagctg ccggtcgcgc tcgtccgcgg gaacgtcatc
     1741 gggaaccatc acgcccgcgg tgatgtccca cggacgctga tgattgaaga gcgtcagcgg
     1801 gatcaggttc gtcaggtgga tcccgaagag gctttcggcg tacttgtggc ccagctgggc
     1861 agtcaccagc gcgccccagt cgccgccctg ggcgccgaag cgctgaaagc cgagcacctc
     1921 ggtcatcagc gtgtgccaga gatcggccgt cctccagaag ttgatcccag gggtcgtgag
     1981 cggggtcgag aagccgaagc cgggcagcga tggcacgatg acctcgaacg catcctcggg
     2041 atctccgcca aacgacgccg gatcggccag ggggcggatc acctcgttga ggtcccagaa
     2101 cgtccagggc cagccgtggc tgagtatcag gggcaccggg ttcgggccgc gccccggctc
     2161 gcgcagaaaa tgcacgggca tgccctcgat ctctacccgg tagtgatcga aagcattgat
     2221 cttgcgctcc accgcgcgcc agtcgtagtc ccgtcgccag aacccgacca ggtcccgcag
     2281 gtaggctccg ttggttccgt aggaccagtc atcgttcgcg aagtcgtcgg gccagccgga
     2341 acgatcgagc cggtcggcca gatcgcgcag cgtctcttcc gggacctcga tgcggaaagg
     2401 ttggggcttc acggcgcttc agtccagata cccgggatcc ctgccgtccg ggaacggtgc
     2461 cgacatgtcc atgatggggc tgtcgccggg cttcaggttc tgatgcgccc gcttccaggt
     2521 cgcaaaagta cccaggatgt gcaccggatc tccgtggatc tcggtgaagc cgccgttcac
     2581 cgagcgcgta tcgaaatacg cggcgttaac gtcgtccgcc cagagctcgc aggcgttcac
     2641 gaaacccaga tccagcatgc gctgacgggc ggcagcgaag tcgttgacaa gacagccgac
     2701 gtgatggaag ccctcttccc ccgcctgata catgtcccga tagatcgacg gggtttcgtc
     2761 gtgctgctgg atgaactgga tctggttttc gcccaggtag ccgaacccgt aggatacgtc
     2821 cgcttcctgg tccgtgcccc gatactggaa cttgtccgtt ttgtggtggg gcacgagcac
     2881 gaaaggcccg gctccgtaga gccggttcca ccggtgagcg gcttcctcga tgtcgttgac
     2941 gaagtacgcg tgctgaaaga tcggcatggt gccgttactc attgctgttg tctccttttc
     3001 ctgtgaaggt cgtcctgagc gcccctagag catctccgag gcgaccagtt ccagcgcttc
     3061 gggctgctgg gtgccgagca gcatgctcgc cacctgaccg ttgccgcccg ccgccttcca
     3121 tctctgcagc ctttcccgaa tgcggtcggc cggacccacc aggtggcagg cgtcgatcag
     3181 ctccgccggc accgcgcgca tggcctcttc gcgccgtccg gccaggaaat gatcctggat
     3241 ctcgacggcg gcgtcttcga acccgagcgc tttggcgtag tcgttgtaga agttcttgtt
     3301 gcgggctccc atcccgccga tgtagagcgc catgtttcca cggatgggcg ccatgcactg
     3361 ctccacgtcg tccccgatga ccacggtgac gaacggcgac acctcgaaac tgctcagatc
     3421 tttgtcgcca gctgcggcaa aacctttccg tatgggctcc tcgaacacgg agaacttttc
     3481 cggatccatc cagatcggga agaacccgtc ggcaacctcc gcggcagtct ccacaccctt
     3541 cggcgtgatg ctcgcggcga agattctgat gtcctcctcg cagtgcagga tgctcttcag
     3601 cggtttgccc agacccgtgg agccggggcc cgtgtaaggg atctggtaca tctcgccatc
     3661 gaaggtgagc ttgtcctcac gggcgaaaat cttgcgcatg atggccacgt actctctcag
     3721 ccgcgtcatg ggcttgccgt acggcacgcc gtgccagcct tccactacct ggggccccga
     3781 agcaccgagg ccgatgatga accggccacc ggagagctcc gccaggctca tggccgtcat
     3841 ggcggccatg gccggcgtcc gagcaggcat ctgcatgatg gcggtgccca ccttgatctt
     3901 ctcggtctgc gcgagaatcc aggtggcggg cgtgaccgcg tcgcctcccc aggcttcggc
     3961 cgtccagacc gagtcgtagc caagggactc ggccttgcga atcgcctcga tgttgatgcc
     4021 cgtcttgcgg ccgatgttgc cgccgagaat gccgagtttc atgtgatatg cctcctttgc
     4081 gagtaatgcg atgagttcag ccagtgagcc ggtccgctgc ctcctgcttg agttggcgct
     4141 tgaagatctt gcccgatgcg gtgcgcggca gcggctgggt ggaaaaagac agatagcggg
     4201 ggatctcgaa gcgtgccagg cggggagcca gaaagttcct gatctcctcc tcgtcgaccg
     4261 ggcggcagat gtagacggtc gcgccgactt cctcccccag gcgctcgtcg ggcaccgcgt
     4321 aaacgcacgc ctctatcacg tccgggtgtt cctgcaacgc cgcctccacg gcgccgcagc
     4381 cgatgttctc accgccccgt atgaccaggt ccttgatacg gtcgacgatg aagaggtaac
     4441 cgtcgtcatc cagataggcc acatccccgg tgcgaagcca cccgtcgacg atcgcctccg
     4501 cagtggcctc cgggcgattc cagtaaccgc ggatgagcga ggtgccgcga acctgcagct
     4561 cgccgcgctc tccgcagggc agctcgcgac cctcgggatc taccacccgc aattccagca
     4621 cggcgctgca gcggcccgag ctttccgggc gatccaggta ttcctgaccg gagatgacag
     4681 tgccgatggc gttggtctcc gtcatgcccc agcccgtgct cggcatggcc ttggcaaagg
     4741 tgcgtcggat cccgcgcacc tgctcgggcg cacgggccgc accgccgccg ccgacccgat
     4801 ccaggcagct cagatcccgg ttggtgcgtg cggcaaactg gacgagatcc cccgtcatgg
     4861 ccggcggagc gacgaaagac gagatgcgct cgcgctcgat gagctcggca gcctcctcga
     4921 cgtcccactt gtacatggaa acgacccgcc gctgagaccg gtaggaggca agatagaccg
     4981 catgggagcc cgtcgcgtga aacagcggca cggcgagcag cgtggccggc tgagactcgg
     5041 gagccggcag atcgacgccc tgcatgtagg cgcccgcgtg cgcgtcgagt tcccaggaaa
     5101 ggagcgcggt gacaaggctg cggtgacagg agaccgcgcc tttggggtgt cccgtggagc
     5161 cggaggtata caaaatggtg gcatcgtcct ccggggcgac gtccaccgcc ggcatgttgc
     5221 ctgccgggac cccggcgagg gcctctcgca gatcccgggc gccggtcccc gcgtcatcgg
     5281 cggcccgcac cgccagaacg gctagacccg ccggcggatc ggcacaaagc gccaatcggg
     5341 caaggcgttc ttcgtctgcc aggaggacct tggcgccgct gtccttcagg ccgtactcca
     5401 tttcctccgg cgtccagagc gcgttcatgg ccaccgcgat ggcgccgatg gacgtgaccg
     5461 cggcaaaggc cgtcatccac tccgggtagt tgcgcatgga gatggcgacc ctatcgccct
     5521 tggcgacccc gtactgttcc atcagcagga tggcgattcg gcaggactct gcccaggcgt
     5581 ccgcaaagga aaggcgctcg ccgccataga cgatgaacgg caggtcgctg gcggtggacg
     5641 cgtatagatc ccgaagaagg ggcggcgcgt tgcgaaaggc acggcagctg cgaccgtaca
     5701 gctcgacgtc cacgagctcg tagggttgac ccggcgcggt cagcgcctcc aaggcctcgg
     5761 atctgctcaa catgtgcctt ccacctctcc cgctgcaaac gatacccgcg ctcgctatgc
     5821 gccgacaact cgccattacg gggattcacg gataaccaaa gcattcgcgc cctatatcct
     5881 caaaatcgtg taaacgtggg aggggtcctc gtgaagccat acggcaaacg acacgcgagc
     5941 tgggtctggc tgaccctttt tctgctgacg ccgaatccgt cgcggagcga ggcgccgacc
     6001 gctaccgcag gcgacgctct ggaccaggcg cagtcctatc agaccattgc aggccgcagc
     6061 caggcccggg tcgaggatct cgacgacgcg gccctggcca tgctctccca gtacaacggc
     6121 gaggtcgatc gctacgagga ccttcgcacg tacaacgaca acctgcggga gctgctggcc
     6181 agccagcgcg ccgagaagga acgactcaat gccgagcttg ccgggataga gatcgtccgt
     6241 caggagatcg tccccctaat ggtgcagatg atcgacgtcc tcgacagctt cgtcgcgctg
     6301 gaccagccgc tcctgatcga cgagcgcacg tcccgggtgg agtcgctgcg aaggaacctc
     6361 acgcgttccg acgtagacct ggccgagcgc taccgccgta tcatcgaggc gtatcagatc
     6421 gaggcggaat acgggcagac catcgaggcg tacgaaggac ccatcgtcat tgacgaccgg
     6481 gaactgaccg tcgactttct tcgggtcggc cgcatcgggt tgtactacct ttccctggac
     6541 cgggccgagg gcggcatctg gaacccagcc gagaagcgct ggacgcccct ggcggaaaat
     6601 tatctggacg atctcgacct cgccgtccgg atggcacgca agcaggctcc gccaaacctg
     6661 atggaccttc ccatgtggac gacgggaggc gaatcatgag gcggacggcg atcacgctgc
     6721 tggccctgct cgcgcccgcg agctgggccg cagcccccac caccctggac gagctcatcg
     6781 actccctgaa ggactcgtcc gccctgcagg ccaagcgact gcaggagcgg gaggcacggt
     6841 tcgtggcagc ccgcaacgag cgcgagcgcc tgctggagga tgcccgcgac cgccgccgcg
     6901 aagccgagcg ggaagcagac cggctgcggg ctgatttcga gggctcagag caggagctgg
     6961 ccgatctgga aacccgtctc gacgagaacg ccggcgatct ccgggagctg ttcacggtgg
     7021 tgcgccagat caccggggac gtggctccaa tggtcgaaag ctcactggtc tcggcagagc
     7081 agggtccgcg caacgctctg ctgaggaggc tggcggagcc cggagccatc cccgccgccg
     7141 aagagctgcg cgagctctgg atgatcctgc tggaggagat gaatcacagc ggccgggtgt
     7201 cccggttcga agcgcccgtg atctccgccg acggcgcgga gcgcacgcgc cgcgtcacgc
     7261 gcatcggcac tttcaccgcc ctggccgacg gcagctatct gcgctacctg ccggagaccg
     7321 ggcgcctcct ggccctcggc cgccaaccgg cgggggtcga cgtgggcgac gtgctggcat
     7381 tcgaggaagc ccgggaaccg ctgcagagag ttgccatcga cccttcccgg ggctccattc
     7441 tctcgctgat cgtgcagacg cccgacgtgc gcgagcgcat cgagcagggc ggcgtcatcg
     7501 gctacatcat actcgcgctc ggcgcgctgg gcctgctgct gggcgtggag cgcctggtga
     7561 ccatcggggt cgccaggcag cggacgcgaa gagtgctcgc ggattccgct gcggccggcg
     7621 accacgtgat cgtggagctc agagagctgg caagggacga ggcgtatctg gccgacgccg
     7681 aggcgctcag cgcaaagatg gacgaggtgg tcaccattgc cagcgaaaag ctgcgccggg
     7741 ggttgcccac cctcgcgatc tttgcagccg tgagccctct tctggggctg ctgggcacgg
     7801 tgaccggcat gatcgagacc tttcaggtca tcaccctgtt cggcgccggc gatccgcgcc
     7861 tgatgtcggg cggcatatcc caggccctga tcaccactca gctgggtctc gccgtcgcga
     7921 tcccgctgct gctgatccag agcttcgtcc aggggcgggc aaacgcgctg atcgccaccc
     7981 tggatgaaac cgcggccgag ctctttgccc agggccgcac gcatcggggc agcgaccatg
     8041 ctgggtgatc ttttcggcaa cagcgacctg atcatggccg gcggctggat catcgtgctg
     8101 atcatggcgc tcacgttcgt catgtggatg atgatcctgg atcgctacgt gtacctgtgg
     8161 cgcgaccgcg gacgagccgt ctcgcgcaaa ttcgcgctgt ggcagcaata ctcgagcccg
     8221 gatcccctga acaacaagcg cctgcgagcc gcgctgaccg ggcagtttct cgaggattcc
     8281 cgtcgctgga tcggcaccat tcaggtcatc accgccgtgc tgccgctgct gggactcctg
     8341 gggacggtca ccggcatgat caagaccttc gacgtgctca ccgcgttcgg cacgggcaac
     8401 attcgcggca tggccgaggg catatcccag gcgctgatca ccaccatggc ggggctgatc
     8461 gcggcgctca ccggcatcta cttcgccaac gatctggaag agcgcatcgc caccgaagcg
     8521 gatcatctcg ccggccggtt caacgagggc tttgccgacc gggggaacgg gaggtgaagc
     8581 tgcacacgcg ggaagaaggc ggcagcgcgg ccatcaacct gacgccgctg atcgacatgg
     8641 tgttcatcct gctcatcttt ttcgcggtga acgccacgtt cgtgaagatc accggcgtgt
     8701 ccgtggacca gcccacggcc aagaccacga gcctgcagcc ggcgagcacg atcctcatcg
     8761 ccgtcaccga tgccggcgag atctggatcg acaagaagaa agtggacaag cggcgcgttc
     8821 gcggcgtggt ggagcgcatg ttcatcgagg ctccggacgc ggccgtggtg gtgctgtcgg
     8881 acgaggagtc acgcacgggc ctggtcgtgg aggtcatcga ccaggtgcgc ctggccgggg
     8941 caggcagggt ggcgctggcg gctgcagagc ccgaggagca gtgatggcgc tgcgccaggg
     9001 tttcatcttc ctcggcgcgc tggccgtcac cctgtcgctc tacgtcatga tggaccgcat
     9061 ggtggggcgt gaccgcaccc gtctgctcag ccccgtggac gcccagtcca tcgagttcgt
     9121 ccgcaccgag atggaggacc gctcacgtac caaggaccgg cggcgcaaac cgccgccaaa
     9181 gccccaggaa atgaagcgcg ttcgcgccga cctgcagacc aacctcaacc gtgcggccga
     9241 cctgcctacg gaaacgctgg cattcaacat caagagcctg ctgggcgaag gcggcggggt
     9301 ggcgctgggt gagcgcctgg tccagggcag cggcgccgct ttggagctgg tcatggccag
     9361 cgacctgacg cccttgagca tgctgccgcc ccagtacccg ccttcggcgc tgatgcgcgg
     9421 cgtcgagggg tgggtggata tggtcttcat cgtccccgaa gagggcggcg tggtggaccc
     9481 gaccatcctc gagtccagac cgggaaccac gttcgacgcg gccgccaagg ccgcggccat
     9541 gaggtggcgt ttccgccccg tcgtccgcaa tggccagccc acgcctatcc gagcccgcat
     9601 ccgcatcgac ttcgagctgc cggagtgaag ggcgcggcgt tg
//
LOCUS       DBNHOX010000301         2376 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1053887, whole genome shotgun sequence.
ACCESSION   DBNHOX010000301 DBNHOX010000000
VERSION     DBNHOX010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1053887"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2376
                     /locus_tag="AC16UZ_06615"
     CDS             <1..>2376
                     /locus_tag="AC16UZ_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR6246393.1"
                     /translation="LAGRYLVLMPNNPRAGGISRRIEGDDRDQLRDALGQLNVPEGMG
                     VIVRTVGVGRSPEEMQWDLDYLLQLWEAIQKADEQERAPKLIYQENNVILRAIRDNLR
                     KDIGEVLIDGKEAFEEAKDFIEQVMPHYSQRVKYYSDPIPLFNRFQIESQIEMAFQHS
                     VRLPSGGSLVIDPTEALASIDINSARATKGADIEETALNTNLEAADEIARQLRLRDMG
                     GLIVIDFIDMTSVKNQRAVENRMREALDADRARVQISRISRFGLMEMSRQRLRPSLEE
                     LTTQVCPRCSGQGRIRDTKSLALGVLRLVEEEALKERSSIVRALVPLSIASYLLNEKR
                     ADVAQIEQRTGTHLVIVPSVNMDTPHFEIHRVRDDHAREVGIVPSYELAESAAAEEAM
                     PLQEAPRPPAPQAAVQAIQPSAPKPAPAPAQAPQPAARKPQSPSQSNRPGLFSRLRDA
                     LFTSGESQAEGSTSTERQAQGERAEDRGKKDDGRRNRQRGNGGQRKDTARHNDGAESR
                     RGQPEEAKGRKRGPKKDSEEGSNARKAKGGERGGERGGERGKGSEKAQDKAKEKGKDK
                     GKDKDKGKDKAAGAGTPPTEEEITRSKRRPRRDRSQVEKSEPANGRPTAAAGGEAAPV
                     ATTTAQPAARAGAATAAPVTETPEAVSSETSAAAVEPAAEQQPQESAKVASGSSTPQG
                     RAANDPRERNIADQPTPAAREKAPASDAAPPTPPAEPVAQAPDVPAASPDKPAAEEPA
                     VAKRPEAKGQPKAAYVEPAPEAPTPEPRPSRAFNDPREVRRREREAELKAEG"
BASE COUNT          533 a          785 c          781 g          277 t
ORIGIN      
        1 ctcgccggcc ggtatctcgt cctgatgccc aacaacccca gggcgggcgg catctcccgc
       61 cgcatcgaag gcgacgaccg agatcagctc cgagatgcgc tcggtcagct caacgtcccc
      121 gagggcatgg gcgtcatcgt gcgcaccgtc ggggtaggtc gaagccccga ggagatgcaa
      181 tgggacctcg actacctgct gcagctctgg gaggcaatcc agaaggccga cgagcaggaa
      241 cgcgctccaa aactcatcta tcaggagaac aacgtcatcc tgcgcgcaat ccgcgacaac
      301 ctgcgcaagg acatcggtga agtgctgatc gacggtaagg aagctttcga ggaagccaaa
      361 gacttcatcg agcaggtgat gccccactac agccagcggg tgaagtacta ctcggatccg
      421 atccctctct tcaaccgttt tcagatcgaa agccagatcg agatggcttt ccagcattcg
      481 gtgaggctgc cgtccggcgg cagccttgtc atcgacccca cggaagccct cgcatccatc
      541 gacatcaact cggcccgtgc aaccaagggc gccgatatcg aagagactgc gctgaacacc
      601 aatctcgaag ccgccgacga gattgcccgg cagctccgtc tgcgggacat gggcggcctg
      661 atcgtcatcg acttcatcga catgaccagc gtgaagaacc agcgagcggt cgagaatcgc
      721 atgcgggagg ccctcgatgc ggacagggcc cgggttcaga tcagcaggat ttcgcgattc
      781 ggtctgatgg agatgtctcg ccagcgcctg cgtccttccc tcgaagagct aactacccag
      841 gtgtgtccgc gctgcagcgg gcagggtcga attcgagaca ccaagtcgct ggctctcggt
      901 gtgctgaggc tcgttgaaga ggaagcactc aaggaacgca gctccatcgt tcgcgcgctg
      961 gtgccgctga gcatcgcctc gtacctgctc aacgaaaagc gggcggacgt cgcgcagatc
     1021 gagcagcgca ccggcaccca tctggtcatc gtgccgagcg tgaacatgga caccccgcac
     1081 ttcgagattc atagggtgcg ggacgaccat gcccgggagg tgggcatcgt gccaagctac
     1141 gagctggccg agtcagcggc agctgaggaa gcgatgcctt tacaggaagc accccgcccc
     1201 ccggcccctc aggctgcggt tcaggccatt cagccttccg cccccaaacc ggctcccgcc
     1261 cccgcgcagg cgccgcaacc cgccgcccgc aaaccgcagt cgccgagtca gagcaacaga
     1321 cccggcttgt tctcccggct gagggatgcg ctgttcacca gtggagagtc ccaggcagaa
     1381 ggctcgacca gcacggagcg ccaggcccaa ggcgaacgcg ccgaggatcg tggcaaaaag
     1441 gacgacggca gacgcaatag gcaaaggggc aacggcgggc agcgcaagga caccgcccgg
     1501 cacaatgacg gcgctgagag ccgacgcggg cagcccgagg aggccaaggg caggaagcga
     1561 gggccaaaga aggattctga agagggctcc aacgccagga aggcgaaggg cggcgagcgc
     1621 ggcggcgagc gcggcggcga gcgcggcaag ggctccgaga aggcacagga caaggcgaag
     1681 gagaaaggca aggacaaggg caaagacaag gacaagggca aggacaaagc ggccggagca
     1741 ggaactccgc ccaccgagga ggagatcaca aggagcaaac gtcgccccag gcgcgaccgc
     1801 agtcaggtcg agaagtccga acctgccaac ggcaggccca ccgcagccgc cggtggcgag
     1861 gcagcgcccg tggcgaccac cactgcgcag cccgcagcga gggcaggcgc cgcgaccgcg
     1921 gcaccggtca ccgaaacgcc tgaggccgtc tcatccgaga cctccgccgc ggcagtcgaa
     1981 ccggccgccg agcagcagcc acaggagagc gctaaggtcg cttccggctc gtcgacgccg
     2041 caaggccgtg cagcaaacga tccacgtgag cggaacatag ccgatcaacc gacgcccgcg
     2101 gcgcgtgaga aagcgccggc ttcggacgcg gcgccaccaa cgccgccggc tgagcccgtg
     2161 gcacaggctc cggacgtacc ggcagcgagc ccagacaagc cagcggccga agaaccggcg
     2221 gtcgcgaaac gacccgaggc aaaaggccag ccgaaggctg cctacgtcga gcccgcaccc
     2281 gaggcgccaa cgccggagcc gcgcccgtcc cgggccttca acgatccgag agaagtgcgc
     2341 cgacgtgagc gggaagcgga actcaaagcc gagggc
//
LOCUS       DBNHOX010000302         4011 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_795953, whole genome shotgun sequence.
ACCESSION   DBNHOX010000302 DBNHOX010000000
VERSION     DBNHOX010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_795953"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="AC16UZ_06620"
     CDS             complement(<1..872)
                     /locus_tag="AC16UZ_06620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6246394.1"
                     /translation="MAAARSLPYPNRAYAWFVLAVLVVAALIAFIDRQVVAIVVDPMK
                     ADLGVGDAQIGWLYGVFALFYAVAALPIAWLADRKSRKHIIAAGIFLWSLMTIACGLS
                     RSYWNVFLARIGVGIGEATLTPATTSMVGDYFPRDQIPLALSIFQTGAIMGSGIAFII
                     GGVVLNIVEQAEPMVLPLVGELRPWQQTFVYVGAPGMILALLLLLLREPVRRSAGGTG
                     TQQEATFAEIVAFYRRNARTLACHHIGFLSFALMGYAFVFWTVSFFVRVHGYDAADAS
                     QVFGWIFFLTGPLGP"
     gene            complement(905..1681)
                     /locus_tag="AC16UZ_06625"
     CDS             complement(905..1681)
                     /locus_tag="AC16UZ_06625"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246395.1"
                     /translation="MKLEGKVAAITGGTRGIGRGIAEAFLAEGANVVVNGRTPEKGQR
                     ALEEMGVGDAAHFIAGDVRVQADVEAFVDGTIERYGKIDILVNNAGGSSGFAPISELS
                     DEAWNEALDWMLNATFWGTRRALPDMLSRKWGRIINISSVEGLLINKTTVGHYIVNKH
                     AINALTKVVAYENGPDGITSNAILPGAVETDLMTITGAEAAKSLGMTYEQYIDGYAQE
                     AAIKRINTVEEVAAVALLLASDLGGGITGALMEVHGGTAL"
     gene            complement(1746..2357)
                     /locus_tag="AC16UZ_06630"
     CDS             complement(1746..2357)
                     /locus_tag="AC16UZ_06630"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6246396.1"
                     /translation="MRLHESPSPNARRVHVFLKEKGIEAPPTVRVDIRGGENLSDAYR
                     KLNPFGRVPVLELDDGTCLAESVAICRYFEGLHPEPSLFGREPLEMAIIEMWNRRAEI
                     NFMLQATGAFRNLTGFFKDREKVSKEWGAIALGNAADALGRFDTALGTSRFLAGETFS
                     IADITFAVVLQFAQMVKLDLPFDLPNIERYLAEVGSRPSFQES"
     gene            2437..3405
                     /locus_tag="AC16UZ_06635"
     CDS             2437..3405
                     /locus_tag="AC16UZ_06635"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR6246397.1"
                     /translation="MAQTVTLTRRWPAQVEAHLQSLYDLSVDTTDRPRTRDELAAALA
                     SADALCPTVTDRIDADLLGSAEVRTRIIASFGVGHEHIDLDAARARGIVVTNTPGVLT
                     DCTADLTLALLLGIARRIGEGEREVRGGSWTGWRPTHMMGTRVTGKTLGIVGLGRIGV
                     AVARRARLGFGMRVLFHNPTPPPPERIAAAGAEPCSTLVELLQRSDFVSLHCPATVDT
                     HHLINAERLAQMPAHAYLINTARGTVVDSEALATALRERRIRGAGLDVYEGEPEVPAG
                     LLELDNVILLPHLGSATEETRTAMGMRVAENLAAFFAGSEPPDRLV"
     gene            3447..3860
                     /locus_tag="AC16UZ_06640"
     CDS             3447..3860
                     /locus_tag="AC16UZ_06640"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR6246398.1"
                     /translation="MTSSADDIAAIEKLQADWNAAVEAGSVDGYAAVLDDDIELLPTD
                     AETIRGIGNYRAMLDGVFTTDDFRIEIAVPATIEVDGDMAWSRYDYVIHRTAKDGSGT
                     FSSYRKFLDVMRRQDDGTWRVYKHIWNYNDPDVVP"
BASE COUNT          747 a         1337 c         1277 g          650 t
ORIGIN      
        1 gggcccaggg ggcccgtcag aaagaatatc cacccgaaca cctgggacgc atccgcggcg
       61 tcgtaaccgt gaacgcgaac gaagaacgag accgtccaga acacgaacgc gtagcccatc
      121 agcgcgaacg acaggaaccc gatgtgatga cacgcgagcg tgcgagcatt gcgccggtag
      181 aaggcgacga tctcggcaaa ggtcgcttcc tgctgggtcc cggtgcctcc cgccgaacgg
      241 cgcaccggct ccctgagcag cagcaacagc aaagccagga tcatgcccgg cgcccccacg
      301 taaacgaacg tctgctgcca agggcgcagc tctccgacca aagggagcac catgggctcc
      361 gcctgctcga cgatgttcag gaccacgccg ccgatgatga aggcaatccc cgatcccatg
      421 atcgcccctg tctgaaagat gctgagcgcc aggggaatct gatcccgcgg aaagtagtcg
      481 ccgaccatcg aggtggtggc cggagtgagt gtcgcctcgc cgatgcccac cccgatgcgg
      541 gccaggaaca cgttccagta gctgcgcgac agcccgcagg caatggtcat cagggaccaa
      601 agaaagatgc cggccgcgat gatgtgcttg cggctcttgc ggtcggccag ccaggcgatg
      661 gggagcgcgg caaccgcgta gaagagcgcg aaaacgccgt acagccaacc gatctgggcg
      721 tctcccacgc ccaggtccgc cttcatggga tccaccacga tggcgaccac ctgccggtcg
      781 atgaaggcaa tgagcgcggc gaccaccaga acggccagca cgaaccacgc gtaggcccgg
      841 ttcggatacg gcagagaacg cgcggcggcc aatgcgccgc gttccccggc tcccgagggc
      901 gtgctcagag cgcggtgccc ccatgcactt ccatgagcgc accggtgatg ccgccgccca
      961 ggtcggaggc cagcagaagg gccaccgccg cgacttcctc cacggtattg atccgcttga
     1021 tggccgcctc ctgggcgtag ccgtcgatgt actgctcgta ggtcatgccg agggacttgg
     1081 cggcttcggc accggtgatg gtcatcaggt cggtttccac agcgcccggc aggatggcgt
     1141 tggaggtgat cccgtcggga ccgttctcgt aggccaccac cttggtgagc gcgttgatgg
     1201 cgtgcttgtt gacgatgtag tggcccaccg tcgtcttgtt gatgagcagc ccctccacgg
     1261 aggagatgtt gatgatccgg ccccacttgc gggacagcat gtcgggcagc gcccgtcgcg
     1321 tgccccagaa agtcgcgttg agcatccagt ccagcgcttc gttccacgcc tcgtcgctga
     1381 gctcggaaat cggtgcaaag ccggatgagc cgccggcgtt gttcaccaga atgtcgatct
     1441 tgccgtagcg ctcgatggtg ccgtccacga aggcttccac atcggcctga acgcgaacgt
     1501 cgccggcgat gaagtgcgcg gcgtctccga ctcccatctc ctccagggca cgctggccct
     1561 tttcgggcgt tcgcccattc acgacgacat tcgccccctc ggccagaaag gcctccgcga
     1621 tgccgcggcc gatgcccctc gtcccgccag tgatggcggc cactttgcct tccagtttca
     1681 tccgtcggtt tcctcccgtc gtttgaccat acccctctgg ccgccgctag ggcgacgcgc
     1741 tgagatcagc tctcctggaa gctgggtcgc gatcccacct ctgccagata tcgctcgatg
     1801 ttcggcagat caaaaggcag gtccagcttg accatctggg caaactgcag caccaccgca
     1861 aaggtgatgt cggcgatgct gaacgtctcg ccggccagga accgggaagt gccgagcgcg
     1921 gtgtcgaagc gcccgagcgc atctgcggcg ttgcccagcg cgatggcgcc ccattccttg
     1981 gagaccttct cacggtcctt gaagaagccg gtcaggtttc ggaacgcgcc ggttgcctga
     2041 agcatgaagt tgatctcggc ccggcggttc cacatctcga tgatcgccat ctccagaggc
     2101 tcccggccga acagcgaggg ctccggatgc aacccctcga agtagcgaca gatggcaacg
     2161 gattccgcca ggcaggtgcc gtcatccagc tccagcacgg gaacccggcc gaaggggttg
     2221 agcttgcggt aggcgtcgct gagattttcg cccccccgga tgtccaccct caccgtgggc
     2281 ggcgcttcga tgcccttctc tttcaggaaa acgtggaccc ggcgcgcatt tggcgatggc
     2341 gactcgtgca gcctcatcag aactctcccc gcttcagcga tgcggacaat tgtacgcgat
     2401 gtgcaatacc atgattcgct tccgaggaca aagcatatgg cccagacagt cacgctcacc
     2461 cggcgctggc ctgcgcaggt ggaggcccat ctccagtcgc tttacgacct ctcggtagac
     2521 actaccgacc gaccgcgcac ccgcgacgag ctggctgccg cgcttgctag cgcggacgcg
     2581 ctctgcccca ccgtgacgga ccggatcgat gccgacctgc tgggctcagc tgaggtgcga
     2641 acgagaatca tcgcgagctt cggcgtcggt cacgagcaca tagacctgga tgccgcccgg
     2701 gcccggggga tcgtggtcac caacacgccc ggagtgctga ccgactgcac ggccgatctc
     2761 accctggcgc tgctgctcgg tatcgcccgc cgtatcggcg agggcgaacg ggaggttcga
     2821 ggcgggtcct ggacgggctg gcgtccgaca cacatgatgg gcacgcgggt gacgggcaag
     2881 acgctgggca tcgtcgggct cggcagaatc ggcgtagccg tggcccggcg cgcccggctg
     2941 ggtttcggga tgcgcgtgct cttccacaac ccgacgccgc caccaccgga acgaatcgcg
     3001 gctgccggcg ccgagccatg ctccaccctt gtcgagttgc tgcaacgctc cgacttcgtg
     3061 tcgctgcact gcccagccac cgtcgacacc caccatctga tcaacgctga gcggcttgca
     3121 cagatgccgg cccacgccta tctcatcaac acggcccgag gcacggtggt ggattccgaa
     3181 gccctggcga cggcgctgcg cgagcgccga atccgcggcg ccgggctcga cgtctacgag
     3241 ggcgaaccgg aggtacccgc cggcctgctc gagctcgaca acgtcatcct gctgccgcat
     3301 ctgggcagcg ccaccgagga gacgcgcacc gccatgggca tgcgggtcgc cgaaaatctg
     3361 gccgcgttct tcgcagggag cgagcctccg gatcggcttg tctgataaac tttcgcgctt
     3421 cgcacaggca acgtgttggg ggaaccatga cgtcatcagc ggacgacatc gccgccattg
     3481 agaagctcca ggccgactgg aacgcggccg tcgaggccgg cagcgtcgac ggttacgccg
     3541 ccgtgctgga cgacgacatc gagctgctgc ccaccgatgc ggaaaccatc aggggcatcg
     3601 gcaactaccg ggccatgctc gacggggtgt tcaccaccga cgacttccgc atcgagatcg
     3661 cggtccccgc caccatcgag gtggacggcg acatggcctg gtcccgctac gactacgtca
     3721 ttcaccgcac cgccaaggac ggcagcggga cgttcagcag ctatcggaag ttcctggacg
     3781 tcatgcgacg ccaggacgac ggcacctggc gggtgtacaa acatatctgg aactacaacg
     3841 atcccgatgt agtaccctag ggaaattcga agagggagga ctcaaaaatg tcgagagtac
     3901 tgataagcgg cgcgagccgg ggaattggac tggagctggt gaagcagtac gcggcggccg
     3961 gcgacgaggt gatcgcgtgc gtgcgggaca cgggcaatgc cgccctgctt g
//
LOCUS       DBNHOX010000303         2038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_603910, whole genome shotgun sequence.
ACCESSION   DBNHOX010000303 DBNHOX010000000
VERSION     DBNHOX010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_603910"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="AC16UZ_06645"
     CDS             <1..180
                     /locus_tag="AC16UZ_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246399.1"
                     /translation="MGHLISDDVLEAFAVVGEPQQIAGQIISRYGDIVDRTSAAYAHI
                     SKEHRQEIIAELRLS"
     gene            438..1091
                     /locus_tag="AC16UZ_06650"
     CDS             438..1091
                     /locus_tag="AC16UZ_06650"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR6246400.1"
                     /translation="MPTVATARQNENRKEAKRAKVRWYTPFLFVLHSLAIVGPVLLAA
                     GFAHRVEDAAYRTFAFAVPAPENSYVTWNSEVSEFGTKVSQAFGVRPSTATEFAGWIL
                     EASTRQELEPELLASLVLTESSFRKNVRSHVGAIGPAQVRPEYWSNFCGIANLHDPAE
                     NIYCGAQVLSHLRDRCGDEGCALHAYNVGLYAPRHKAGHRYVAKIDHYREQLLNLPL"
     gene            1160..>2038
                     /locus_tag="AC16UZ_06655"
     CDS             1160..>2038
                     /locus_tag="AC16UZ_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6246401.1"
                     /translation="MLEVPVLIVGGGPVGLAAALECAYFGVPAMLVERHSGTTWHPKA
                     RNLNSRTMEIMRRLGLEPELQAVGLPEAWTRQIVYADTLSGAELGRMGTGGFESRSSA
                     ASPCAAVLSSQDVFEPIWRGRAEQQPLLDVRYGHQAEILDVDAAGAVVRLTGARGGDV
                     RARYLLAADGAGGAIRQGLGIGLEGPENLGHFINVYYRANLDRVVAHRPAVLYFTANE
                     AGRGVFQPLDGRKRWLSQIAYDGAADTRAGFDGERCAQWIRNAAGEPDLKVEILGISH
                     WTMNATVAERYRQGAAF"
BASE COUNT          377 a          626 c          664 g          371 t
ORIGIN      
        1 atggggcacc tcatcagcga cgacgtgctc gaggcgttcg ccgtggtggg cgagccgcag
       61 cagatcgccg gtcagatcat ctctcgctac ggcgacatcg tcgatcgcac ctcggctgcc
      121 tatgcgcaca tttccaaaga acaccgtcag gagatcatcg ccgagctgag attgtcataa
      181 tgttctaaaa aatgaaaaat tgattacttt taggtataaa aagtaatcga gcggtgttct
      241 gattcgcgat tcaagcgtga cggggcgtgg acggtaagga attcctcaca aaagtccgtg
      301 cacggcccgc atttttgcat tctcccaacg tttcggtggc aacctgccaa cgacttcgca
      361 cgaaatttgg gttcattgtg gcatttcagg tctttagaat gcgcagccgt acacagtcga
      421 tatgaacacg agacgaggtg ccaacggtgg caacggcaag gcagaacgag aaccggaagg
      481 aggcgaagcg cgcaaaagtg cgctggtaca cgcccttcct cttcgtgctg cacagcctgg
      541 cgatcgttgg cccggtgctg ctggccgcag gctttgcgca ccgcgtcgag gacgccgcct
      601 accggacttt cgcgtttgcg gtgccagcgc cggaaaactc ctacgtgacc tggaattccg
      661 aggtgagcga gttcggcacc aaggtgtccc aggcgtttgg cgttcggccg agcacggcca
      721 cggagtttgc agggtggatc ctggaggcat cgacacggca ggaactcgag cctgaattgc
      781 tggccagcct ggtgctgacc gagagctcgt ttcgaaagaa cgtccgctcc cacgtggggg
      841 ccatcggccc ggcccaggtg cggccggagt actggagcaa cttctgcggc attgccaacc
      901 tccacgaccc cgcggagaac atctactgtg gtgcccaggt gctttcccat ctgcgggacc
      961 gctgcggtga cgaagggtgc gcgctgcatg cctacaacgt tggcctctac gccccgcggc
     1021 acaaggccgg acaccgctac gtcgccaaga tcgaccacta tcgcgagcag ctcctcaacc
     1081 tgcctctttg atctgacgcc tctagcctag cccgcccgga agggcgtacc ctcttggctg
     1141 gcgccccagg ggatgcgcgt tgctcgaagt tcccgttctc attgtcggag gtggccccgt
     1201 ggggctggcc gcagcgctcg agtgcgccta tttcggcgtg ccggcgatgc tcgtcgagcg
     1261 gcattccggc accacctggc atcccaaggc gcgtaacctc aactcccgga ccatggagat
     1321 catgcgtcgg cttggcttgg agcccgagtt gcaggccgtt ggccttcccg aggcctggac
     1381 gcgccagatc gtctatgccg atacgctcag cggtgccgag ctcggacgca tgggcaccgg
     1441 ggggttcgag agccgaagct cggcagccag cccgtgcgcg gcggttttga gctcacagga
     1501 cgtgttcgag cccatatggc gagggcgggc ggagcagcaa ccgctcctgg acgtgcgcta
     1561 cgggcatcag gcggaaatcc tcgacgtgga cgccgcagga gccgtcgtgc gcctcaccgg
     1621 agccagaggg ggcgatgttc gcgcccgcta cctcctggcg gcggacgggg ccggtggtgc
     1681 gatccggcaa gggctgggca tcgggctgga agggcccgag aatctcggcc acttcatcaa
     1741 cgtctactat cgagccaatc tcgaccgagt ggtggctcat cgcccggcgg tgctttactt
     1801 cacggccaac gaagcggggc gcggcgtgtt tcagcccctg gacgggcgca agcgctggct
     1861 gtcgcagatt gcctacgacg gcgcggcgga cactcgcgcc gggttcgatg gcgagcgatg
     1921 cgcacaatgg attaggaacg ccgcggggga acccgacctc aaggtcgaga tccttggcat
     1981 atcccactgg accatgaacg cgacggtggc cgaacgatac cggcagggcg cggcgttt
//
LOCUS       DBNHOX010000304         1505 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_539212, whole genome shotgun sequence.
ACCESSION   DBNHOX010000304 DBNHOX010000000
VERSION     DBNHOX010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_539212"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="AC16UZ_06660"
     CDS             <1..1156
                     /locus_tag="AC16UZ_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR6246402.1"
                     /translation="DRVAGADIEVRMALIGSALAMYAEYPATGIGLFVFPLLYRFQRP
                     VVDQDSAGLYVHNDYLQLLLEGGPLLFCAMVTFAVVVVIVFFRRLAALPGDEAFARLG
                     LTMGVGAALAHALVNFVVYSPALGFVLGLTVAASFRDSKGSGSVDSTQARPIRVGVTL
                     AVLFGWTAWLYLGLDTATAGILQNQRYVPFTEEVRKSPERMLRFAGMARSLNADRGVP
                     LLAEAVLLDNKLRQNPESRYLGEETLNAFRRAGRADPWNTLTLLLMADFIMRNPSVLP
                     LLENGEAPEELLLRTLVMDPQFVPGIDALMDKLGDEGRHAEAEALLRLRIYPWLRLLA
                     RQNKPAAARYAEALLGMARARGDSEFVSELEARQQEVENTQPVIAQRWFF"
     gene            complement(1163..>1505)
                     /locus_tag="AC16UZ_06665"
     CDS             complement(1163..>1505)
                     /locus_tag="AC16UZ_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246403.1"
                     /translation="PKWVTVEIPHDPWISQEIRMSAMRKGTPLRTLADRYAPFSVEDV
                     DLEREAYPLSELVDRDQLTQQIPLWLDEHGGTLEDYAFVRLATRYRYVFLGIDRRDGA
                     IAGVLGTPAPL"
BASE COUNT          238 a          448 c          490 g          329 t
ORIGIN      
        1 ggaccgtgtt gcaggtgccg atattgaggt ccgaatggct ttgattggct cggcgctggc
       61 catgtatgcc gagtatcctg cgaccggcat cgggttgttc gtctttcccc tgctctatcg
      121 gttccagcgc ccggtcgtag accaggattc ggccggacta tatgttcaca acgattatct
      181 gcagctgctg ctggaaggcg gcccgttact gttctgcgcc atggtgacct tcgcggtcgt
      241 cgtggtcatc gtgttttttc gccgccttgc cgccttgcct ggagatgagg ctttcgccag
      301 gctcgggctg accatgggcg ttggtgccgc gctcgcgcac gccctggtga acttcgtcgt
      361 ctactcaccc gctctcggct tcgtgctggg tcttactgtg gcagcgtcgt ttcgtgattc
      421 gaaaggatcc gggtccgttg actcgactca ggcgcgaccg attcgtgttg gggttacgtt
      481 ggcggttctg tttggatgga cggcctggct ctatctcggt ctggataccg ccacagccgg
      541 catactgcag aaccagcgct acgtgccctt cacggaggag gtgcgcaaga gtccagagcg
      601 catgcttcgc tttgcgggta tggcccggtc cctcaacgca gatcgggggg tgcccctgct
      661 ggcggaagcc gtgctgctgg acaacaaact gcgacagaac ccggagtcca ggtatctggg
      721 cgaggagacg ctgaatgctt ttcgccgggc ggggcgggct gacccgtgga acacgctgac
      781 gctgctcttg atggccgact tcatcatgcg caacccgtcg gtattgcccc tgcttgaaaa
      841 cggcgaggcg ccagaggagc tgctgctgag gacgctggtc atggaccctc agttcgtgcc
      901 gggtatcgac gcgctcatgg acaagctcgg cgatgagggg cgtcatgccg aagccgaggc
      961 cctgctgcgc ctgcgcatat atccctggct tcgtttgctg gctcgtcaaa acaagccggc
     1021 ggccgctcgt tacgccgagg cgttgctcgg catggcccgg gcacggggcg atagtgagtt
     1081 tgtcagcgag ctcgaggcgc gtcagcagga agtggaaaac acccagcccg tgatcgcaca
     1141 gcgctggttt ttttgagtcg gctcacagcg gagcgggcgt tcccaacacg ccggcgatcg
     1201 ccccgtcccg cctgtcgatc ccgaggaaaa cgtaccggta tcgggtcgcc aatcgaacga
     1261 acgcgtagtc ctcgagggtg ccgccgtgct cgtcaagcca gagggggatc tgctgagtca
     1321 gctgatcgcg gtcgacgagt tcgctcaggg gatacgcttc cctctccagg tcgacatcct
     1381 ctacggaaaa cggtgcgtac cgatcggcaa gcgtgcgcag aggtgttccc ttcctcatgg
     1441 cgctcatgcg tatttcctgc gagatccacg ggtcgtgtgg aatttccacc gtgacccact
     1501 tgggg
//
LOCUS       DBNHOX010000305         1954 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_604022, whole genome shotgun sequence.
ACCESSION   DBNHOX010000305 DBNHOX010000000
VERSION     DBNHOX010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_604022"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="AC16UZ_06670"
     CDS             <1..189
                     /locus_tag="AC16UZ_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246404.1"
                     /translation="PETHTTYRYPHDQHLQALIRQCGIIEGSESWEVAGRYNIPRVSS
                     KQRALLARYAVEDQGDQE"
     gene            340..>1954
                     /locus_tag="AC16UZ_06675"
     CDS             340..>1954
                     /locus_tag="AC16UZ_06675"
                     /inference="COORDINATES: protein motif:HMM:NF014825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HIR6246405.1"
                     /translation="MKTLFLPENGFEDLVGRAVRMVLSAESFDDFIDWLRAELVRESA
                     AGAHPLDAQGARALGTALGRAIWGATPAPHNDFRPRALPEPGRNEPCFCGSGLKFKRC
                     CGDGPRLPALRAEELWPIVLEMLPHSGRKAALDSGRVPLAGLALLAVQAIDEGRPKFA
                     IGLLEPLFTGQIAGVDELHDDALNTLCNAYDDMGYHRKKLALLERIRSEAPRSPLRSG
                     ACQRLAAMAIDRGDSTQAWARFRQAQRDDPDSPSVGLLEIQLLMSEGRVDEARERARF
                     WRRRLLRSGDAAFEGPIAFLSAIIDDPHAALADFMIDVNEGAGQRLHEALRELPDRPL
                     PLYRIVPDAEIEPSASSPDALAARLLGMGLPRDQIDALMGDLLAQLENMEPAESAGGE
                     GAEGGVPEGDDAYQLVPPPTIGVLEADWHAVYPVDKPFSVQLAPYADEDPWEPAIEDR
                     WMAFLQRHPEAFDSIDILDDLASAIELHPAGDMLGVDVSLQQPLVERAERILCRALDA
                     AEAPENPQLPWLDPVNRPALRCLFRALVLA"
BASE COUNT          341 a          636 c          646 g          331 t
ORIGIN      
        1 ccggagaccc acacaaccta tcgatatccc cacgatcagc atctgcaggc tttgatacgc
       61 cagtgcggca tcatcgaggg aagcgaatcc tgggaggtgg ccggccgata taacatccca
      121 agagtcagct cgaagcaaag ggcattgctc gccagatacg cagttgagga tcaaggcgac
      181 caggaatgac ccgaagcacg tcatctccgc gttgaacggc gacaacagtc gccagggagc
      241 ggcctgaaca aaagggctgg tgcgcacctt tccaacacgc gtgggtcacg tggcacaatg
      301 tacgtctcga gtctccaacc aagtcgggac ttgaatgccg tgaagaccct gttcctacca
      361 gagaacggtt tcgaagacct ggtcggtcgt gccgtgagga tggtcctctc cgcggagtcg
      421 ttcgacgact tcatcgactg gcttcgggcc gagcttgttc gcgagagcgc cgccggggcg
      481 cacccgctgg acgcgcaagg cgctcgcgcg ctcgggaccg ccctcgggcg ggcgatctgg
      541 ggcgcgacgc ctgccccgca caacgatttc aggcccaggg ccctgccgga accaggccgc
      601 aacgagccgt gtttttgcgg ctccggtttg aaattcaagc gttgctgcgg cgatggcccc
      661 cggctgcccg cgctgcgtgc cgaggaactc tggccgatcg tgctcgagat gctgccgcat
      721 tcgggacgca aggcggcgct cgactccggg cgggttcccc tcgcgggcct cgccctgctc
      781 gccgttcagg ccatcgacga ggggcgcccg aaatttgcga tcggcttgct cgaaccgctt
      841 ttcacgggcc agatcgctgg cgtcgacgaa ctgcacgacg acgccctcaa cacgctgtgc
      901 aacgcgtacg atgacatggg ttaccaccgc aagaaacttg cgctgctgga acggatcaga
      961 tctgaggcgc cgcgctcgcc gttgcgctcg ggcgcctgcc agcgactggc ggcgatggcc
     1021 atcgaccgcg gcgactccac tcaggcctgg gcgaggtttc gccaggcaca gcgggacgat
     1081 cccgactcgc cttccgtcgg gctcctggag atccagttgc tgatgtcaga gggccgggtg
     1141 gacgaggccc gggaacgtgc ccgcttctgg cgtcggcgcc tgctgcgcag cggcgatgct
     1201 gcgttcgaag gaccaatcgc gttcctgtcc gcgattatcg acgacccgca tgcggcactg
     1261 gccgatttta tgatcgacgt caacgagggc gccggtcagc gcctgcacga agccctgcgt
     1321 gagctgcctg accggccgct gccgctgtac cgaattgtgc ctgatgcaga gattgagcca
     1381 agcgcttcga gcccggacgc gctcgcggcc cggctattgg gcatgggact gccgcgggac
     1441 cagatcgacg cgctgatggg cgatctgctg gcgcagctcg agaatatgga gcccgccgaa
     1501 tccgcaggcg gggaaggggc cgaaggcggc gttcccgagg gcgacgatgc gtatcaattg
     1561 gtgccgcccc ccaccatcgg tgtgcttgaa gccgactggc atgcggtcta tccggtggac
     1621 aagccttttt cggtgcagct ggcgccgtac gccgacgagg acccgtggga accggcaatc
     1681 gaggatcggt ggatggcctt tctccagcgg cacccggaag cgttcgacag catcgacatc
     1741 ctggacgacc tggcgagcgc catcgaattg catccggcgg gtgacatgct tggagtcgac
     1801 gtcagcctgc agcaaccgct cgtcgagcgc gccgaacgca tactgtgcag ggccctcgac
     1861 gcggcagaag ccccggagaa tccccagctc ccctggctcg atcccgtcaa ccgcccggcc
     1921 ctgcggtgtc tgttccgggc attggtgctg gctg
//
LOCUS       DBNHOX010000306         3221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1118205, whole genome shotgun sequence.
ACCESSION   DBNHOX010000306 DBNHOX010000000
VERSION     DBNHOX010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1118205"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="AC16UZ_06680"
     CDS             <1..435
                     /locus_tag="AC16UZ_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246406.1"
                     /translation="LEIPFVFGSFSGGLGLGYLYHESEGRHALAESITSYWTEFAYNG
                     DPGTGRDGGEVRWLPWDRNGATSIVLDTLEDKGIYMTTERVTTDSVKTAIATDPDVPD
                     QRERCLLYYRAFRWGSQFDVDEFAGFGDGGCAAYDPAALGDF"
     gene            complement(458..1690)
                     /locus_tag="AC16UZ_06685"
     CDS             complement(458..1690)
                     /locus_tag="AC16UZ_06685"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6246407.1"
                     /translation="MARGPTTVSTTFQAGWRSPLMLLMLMSVAMPVAFNAWSALLNNF
                     VVERAAFTGVEIGILQSLREVPGFLAFTTIFVLLVVREQTFAVLSLAMLGLGVALTGF
                     LPSEYGLYFTTVVMSTGFHYFETLKQSLSLQWLHRDEAPGMLGRLLAVGSLTSLVTYG
                     VLWVLLELFRLDYVWNFLISGGVCVGLAVVMWLWFPHFETKTPQTKRLVLRRRYWLYY
                     ALTFFSGARRQIFVVFAAFLMVEKFGYSAAQVTLLFLINYAFNWAFAERIGRLIGRFG
                     ERAALTFEYIGLIGVFVGYAFVSDPYVAAGLYVVDHMFFALAIAMSTYFQKVADPADI
                     ASSAGVSFSINHIAAVVVPAALGLLWIHSNALVFLIGAGFAVCSLLLSQLIPERPAPG
                     NEIARGRLFPQPADSAGR"
     gene            1890..2255
                     /locus_tag="AC16UZ_06690"
     CDS             1890..2255
                     /locus_tag="AC16UZ_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR6246408.1"
                     /translation="MPDWKDNEHIYLQLKDLVLRQIMLGTLLEGEPVPSVRQVASQER
                     INPLTVSRAYQMLVDEGLLEKRRGVGMFVCEGAREQALKQERTRFLEEDWPEVVERIA
                     LLGLTRDELLNTAAEEGRQ"
     gene            2252..3106
                     /locus_tag="AC16UZ_06695"
     CDS             2252..3106
                     /locus_tag="AC16UZ_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6246409.1"
                     /translation="MSLITARGLAKSFGDVRALDGLDLTVEAGRIVGLIGPNGSGKTT
                     ALKAILGLSHLDAGTLDVLGMNPATSRKALMQRTAYIADVGILPRWMRVSQLLDFVAG
                     VHPGFRREVAEAHLESTDVRPDKRINGLSKGMNVQLHLALVLAIDAELLVLDEPTLGL
                     DIIYRQKFYDALLGDYFDGNRSILVTTHEVREIEHILTDVVFIHHGRSLLSLSMESIH
                     DEFAKVVAQPTQAGAVRSLGPIAERTTLSGAEFVFRGADRDALGSYGEVSTPSLPELF
                     IAIMGDNA"
BASE COUNT          642 a         1055 c         1046 g          478 t
ORIGIN      
        1 ctggagatcc ccttcgtctt cggttccttc tcgggcgggc tggggctcgg ctacctctac
       61 cacgaaagcg aaggccgcca cgcgcttgcc gagagcatca cttcctactg gacggagttc
      121 gcctacaacg gcgacccggg cacgggacgg gacggcggtg aggtccgctg gttgccctgg
      181 gaccggaacg gcgccacgtc catcgtgctg gacaccctcg aggataaggg catctacatg
      241 accaccgagc gcgtgaccac agactcggtg aaaaccgcca tcgcgacgga cccggacgtc
      301 cccgatcagc gcgagcgctg cctgctctac taccgggctt tccgatgggg atcgcagttc
      361 gacgtcgatg agttcgccgg attcggcgac ggcggctgcg ccgcctacga ccccgctgcc
      421 ctgggcgact tctgaccgat ctccagggga ggcggcgtca gcggccggcg gaatcggccg
      481 gctgcggaaa cagccggccc cgggcgatct cgttgccggg ggccgggcgc tcggggatca
      541 gctgggagag cagcagcgag cagacggcga aaccggcgcc gatgagaaag accagggcgt
      601 tggagtggat ccagagcagg cccagagcgg ccggcaccac caccgcggcg atgtggttga
      661 tggagaagga caccccggcg gagctggcaa tatccgccgg atcggccacc ttctggaagt
      721 aggtgctcat ggcgatggcc agcgcgaaga acatgtggtc caccacgtac agaccggctg
      781 ccacgtacgg gtccgacacg aaggcgtagc ccacgaacac gccgatgagg ccgatgtact
      841 cgaaggtgag cgccgcccgc tcgccgaagc ggccgatgag ccggccgatg cgctcggcga
      901 acgcccagtt gaaggcgtag ttgatcagga acagcagcgt cacctgggcc gccgagtagc
      961 cgaacttctc gaccatcaga aacgccgcga agaccacgaa gatctgccgc cgggcgccgg
     1021 agaagaacgt cagcgcgtag tagagccagt agcggcgccg cagcaccagg cgcttcgtct
     1081 ggggcgtttt cgtctcgaag tggggaaacc acagccacat gaccacggcc agccccacgc
     1141 agacgccacc cgagatcaga aagttccaga cgtagtccag ccggaacagc tcgagcaaca
     1201 cccatagcac gccgtaggtc accagcgagg tgagcgaccc cacggcaagc agccgtccca
     1261 gcatgccggg cgcctcgtcc cggtgcagcc actgcaggga aagggactgc ttgagcgtct
     1321 cgaagtaatg aaagccggtg gacatgacca cggtggtgaa ataaagaccg tactccgaag
     1381 gcagaaagcc ggtaagcgcg acgccgagcc ccagcatggc cagtgacagc acggcaaacg
     1441 tctgctcgcg caccacgagg agtacgaaga tggtggtgaa cgccaggaaa cccggcacct
     1501 cgcgcaggct ctgcagaatg ccgatctcca cgccggtgaa ggccgcacgc tccaccacga
     1561 agttgttgag cagggcagac caggcgttga acgcgacagg catggccacc gacatcagca
     1621 tcagcagcat cagcgggctg cgccatcctg cctggaacgt ggtggagacg gttgtgggtc
     1681 ctcgggccaa tgcggtgcgg tccagtgata tgcccggctg ggcgaagggg cgattctaaa
     1741 ggaggcgccg ccggaactcg acgccatgaa cgggcccaga aaccgggccc tgatggccgc
     1801 aggggcccga ataagccggg gttgcccccg gaatcgttat ggtgttatgg ttatgtataa
     1861 cactactgca cataaagagc gacggccccg tgcccgactg gaaggacaac gagcacatct
     1921 atcttcagct gaaggacctg gtcctgcggc agatcatgct gggcacgctg ctcgaaggcg
     1981 agccggtccc ctcggtgcgc caggtcgcaa gccaggagcg catcaatccc ctaaccgtct
     2041 cccgcgccta ccagatgctg gtggacgagg gccttttaga aaaacgccgc ggcgtgggca
     2101 tgttcgtctg cgagggtgcc agggagcagg ccctgaaaca ggagagaacg cgttttctcg
     2161 aagaggattg gcccgaggtg gtcgagcgca tcgccctgct gggcctgacc cgggacgagc
     2221 tcctgaacac cgctgccgag gagggccgcc aatgagcctc atcaccgccc gcggtcttgc
     2281 caaatccttc ggcgacgtgc gcgccctgga cggcctcgac ctcaccgtgg aggccgggcg
     2341 catcgtcggc ctgatcggcc ccaacggctc cggcaagacc accgccttga aagccatcct
     2401 cggcctgtct cacctcgacg ccggcaccct cgacgtactc ggcatgaacc cggcgaccag
     2461 ccggaaagcg ctgatgcagc gaacggccta catcgccgac gtgggcatat tgccccgctg
     2521 gatgcgcgtc tcccagctgc tggacttcgt agctggcgtg catcccggct tccgccgcga
     2581 ggtagcggaa gcccatctcg aatccaccga cgtccgtccg gacaaacgaa taaacgggct
     2641 gtccaagggc atgaacgtcc agctgcacct ggccctggtg ctggccatcg acgccgagct
     2701 gctcgtgctg gacgagccga ccctggggct cgacatcatc taccggcaga aattctacga
     2761 cgcgctgctg ggcgactact tcgacggcaa tcgcagcatt ctggtcacca cccacgaggt
     2821 ccgcgagatc gagcacatcc tcacggacgt ggtgttcatt caccacggca ggagcctgtt
     2881 gagcctctcg atggaaagca tccacgacga gttcgccaag gtggtcgctc aaccgacgca
     2941 agccggcgcg gtgaggtccc tcgggcccat cgcggagcgc accaccttga gcggtgccga
     3001 gttcgtgttt cgcggcgccg acagggacgc cctcggcagc tacggcgagg tcagcacgcc
     3061 gtctcttccc gagctcttca tcgccatcat gggagataac gcatgagcac catggttgcc
     3121 ctgatcagac gcgaatggca ggagcatcag gtgggcctcg gctggtcccc ggtggccatc
     3181 ctggccgtgc tcgcgctcat caccttcatg gccctggcga c
//
LOCUS       DBNHOX010000307         4603 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_153888, whole genome shotgun sequence.
ACCESSION   DBNHOX010000307 DBNHOX010000000
VERSION     DBNHOX010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_153888"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="AC16UZ_06700"
     CDS             complement(<1..365)
                     /locus_tag="AC16UZ_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246410.1"
                     /translation="MKVVATITLAAAFGLSSNASVAAALDERIDAAGAEVVEVVNRSG
                     SVTVTGGSDDVVVVSGSLGSNALGLDLDRDGDRIVVAVRYPEGDSGRNMASTLNISMP
                     RAAGLVVRSISADLEIGGI"
     gene            complement(362..910)
                     /locus_tag="AC16UZ_06705"
     CDS             complement(362..910)
                     /locus_tag="AC16UZ_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246411.1"
                     /translation="MSDDRDDLEFLRRVRGLSGEVEPERDLWPEIRAALDEPGPRAKR
                     WRLPSALAASLLLGAVAASLLFRAGTAPEPAGVPAARPALLEVGLAPELMRSRAQLIA
                     SLDAELARMSPESQAAVAESLKEIEASLAKIHDELEHNPDSRLLLDLLVSTYLRQIAV
                     LGEMDGLARSVARSAQIERTET"
     gene            complement(910..1470)
                     /locus_tag="AC16UZ_06710"
     CDS             complement(910..1470)
                     /locus_tag="AC16UZ_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6246412.1"
                     /translation="MSMHEASSSQDTRADEALALLCQRAGAGDHGAFEELYRASVGRV
                     YGLCLRMTANVTAAEDATQATFVQAWRNLGRFRGEARVTTWLHRIAVNEVLGQQRREA
                     RHQHEPEEAWNEQASETAGASKDLDLERAIAGLPEQARNVFVLCGVYGYGHREAAGLL
                     GIAEGTSKAHYHTARRKLREALAGAE"
     gene            1544..2122
                     /locus_tag="AC16UZ_06715"
     CDS             1544..2122
                     /locus_tag="AC16UZ_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013346.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HIR6246413.1"
                     /translation="MTLDSFPPALTTFVLIGMAELGDKSQLVCMALAARHRAAPVLLG
                     ASLAFLILNALAVFLGAGLAVWLPDRLMAGLVALAFAGFGIHALTAGPGDDPEEHREH
                     RARGIFLTTLSLIFLAELGDKTQIAVAALGATLDPLQVWLGATLALFGLSALGVWIGR
                     SYLKRLPTIWLHRVSGLVFLGVGSLAAWRALT"
     gene            complement(2168..2359)
                     /locus_tag="AC16UZ_06720"
     CDS             complement(2168..2359)
                     /locus_tag="AC16UZ_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246414.1"
                     /translation="MVNNDLVQCLRSGARQRELDPGILDAAADALETLHRRNAELSEM
                     LVQLLPYAAADRLRHKTLE"
     gene            2649..4061
                     /locus_tag="AC16UZ_06725"
     CDS             2649..4061
                     /locus_tag="AC16UZ_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR6246415.1"
                     /translation="MPADREITAEYLIIGGGALGMAIADTLIGESDSSVIMVDRRHKP
                     GGHWHDAYPFVRLHGPSANYGVNSRQLGSDRIDEAGLNRGLFELATGSEIRTYFDEAM
                     RHGLLPTGRMHYLPMHEYNGDGQAVSLISGQATKVSARKKIVDATIAETQVPSRTSPN
                     FSVAEGATLIPPNGLAELGGSASTYVIIGAGKTAIDAVTWLMENDVDPGRLKWIRPRD
                     PWLLNRKMIQPSYDFFENTIGWLIASMEAAANADTVDDIFLHLESAGCMHRIDTSVIP
                     TMYRCAIVSEMELDQLRLVKDVVRMGHVRAIADNRVVLDDGELDLSPGTLFVNCSADG
                     IPRKPVQPIFQPGKIVPQYVRACSPTFSGALVAKVELLFGDDEEKNALTRPTPIPDSP
                     ADWVQIELNQALNSAAWRENKALSQWLAGSRLDQFTAMMLRAFSEGDEAKIGLLDRYR
                     TAIRPAVERMGKMVAASTSA"
     gene            4241..>4603
                     /locus_tag="AC16UZ_06730"
     CDS             4241..>4603
                     /locus_tag="AC16UZ_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024833.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HIR6246416.1"
                     /translation="MKISCLGRMGAISICVAVGLNACTTNPYTGEREASNTAKGAGIG
                     AAIGAVVGVLSGDDADERRKNALIGAGVGALAGGAVGAYMDAQEAKLREQLANTGVSV
                     SRLGDDIVLNMPGNVTFDV"
BASE COUNT          862 a         1523 c         1401 g          817 t
ORIGIN      
        1 tcaatcccgc cgatctccag atcggcgctg atcgatcgca cgacgagacc ggccgccctc
       61 ggcatcgaga tgttcaacgt gctcgccatg ttcctgcccg agtcaccctc cgggtatcga
      121 acggcgacga cgatccgatc gccgtcccga tccagatcca ggcccagggc gttgctgcca
      181 agggagcccg agaccaccac cacgtcatcg gagccacccg taacggtgac cgatccagac
      241 cggttgacga cctcgacgac ctcggcgccg gctgcgtcga tacgttcgtc cagagcggcc
      301 gccactgacg cgtttgagct cagaccgaac gcggcggcca atgtgatcgt tgcgacgact
      361 ttcatgtttc tgtcctctca atctgggccg agcgggccac ggagcgggca agcccgtcca
      421 tttcgcccag cacggctatc tgccgaagat aggtggagac gagcaggtcg agcagcagtc
      481 ggctatcggg gttgtgctcg agctcgtcgt gaattttcgc aagagacgct tcgatctcct
      541 tgaggctctc cgcgaccgcg gcctgggact ccggcgacat ccgcgccagc tccgcatcca
      601 gggacgcgat cagctgcgcc cgggaccgca tgagctctgg cgcgagcccg acctccagaa
      661 gtgccggacg ggcagccggc acgccggccg gctcgggagc cgtcccggcg cggaatagca
      721 gcgacgcggc aacagcgcct agaagcagcg acgcggcaag cgccgaaggc agacgccagc
      781 gcttcgcgcg gggccccggc tcatccagcg ctgcgcggat ttccggccag agatcccgct
      841 cgggctcgac ctccccggaa agcccgcgaa cgcgccgcag gaattccagg tcgtcgcgat
      901 cgtcactcat cattcagctc ccgccagcgc ttctctcaac ttgcgcctgg ccgtgtggta
      961 atgcgccttg ctcgtgcctt ccgcgatacc cagcaggcca gccgcttcac gatggccgta
     1021 cccgtagacg ccgcatagca cgaatacgtt gcgggcctgc tccggcaagc cggcgattgc
     1081 ccgttccaga tccagatctt tcgacgctcc cgcagtttcc gacgcctgct cgttccaggc
     1141 ttcctccggt tcgtgctgat gccgtgcttc ccgacgctgt tgacccagca cctcgttcac
     1201 ggcgatccga tgcagccaag tggtcacgcg ggcctcacca cggaagcggc caagattgcg
     1261 ccaggcctgg acaaaggtcg cctgggtagc gtcttccgcc gcggttacgt tcgccgtcat
     1321 gcgcaggcac aacccgtaga cccggccaac cgatgcccgg taaagctcct cgaaagcccc
     1381 atgatcgccg gcccccgccc gctggcaaag cagcgcaagc gcttcatccg cgcgggtgtc
     1441 ctgtgatgaa ctagcctcat gcatactcat cattatcatc cctgcaagat aagatgcagc
     1501 ctgcgcgaaa agggtttagt cgcgtttgcg gatccaggat ctgatgactc tcgattcttt
     1561 ccccccagcc ctgaccacct tcgtgctcat cggcatggcg gagctgggcg acaagagcca
     1621 gctcgtctgc atggcgctgg cggcacgcca tcgagccgct cccgtgctgc tgggcgcttc
     1681 cctggctttt ctgatcctga acgccctcgc cgtcttcctc ggcgccggcc tggccgtctg
     1741 gctgcccgat cgcttgatgg cgggcctggt ggctctggcc ttcgccgggt tcgggattca
     1801 cgccctgacg gctgggcccg gtgacgaccc cgaggaacac cgggagcacc gtgcccgagg
     1861 aattttcctg actacgctga gcttgatatt cctggcagag ctgggagaca aaacccagat
     1921 tgccgtcgcc gcgctcggcg ccacgctcga cccgctacag gtgtggctgg gagctaccct
     1981 cgcgctcttc gggctgtcgg cgctcggtgt atggatcggg cgcagctatc tcaagcgcct
     2041 gccgacgatc tggcttcacc gtgtgagcgg gttggtcttc ctgggggttg gttcgctcgc
     2101 ggcgtggcgg gcgctgacct gaacggatca aagctcgaga cccacgcccg ctgctgccag
     2161 cagcgcatca ctcgagcgtc ttgtggcgca ggcggtcggc ggccgcgtac ggcaggagct
     2221 gaacgagcat ctcggacagt tccgcattac gcctgtgcag cgtctccaga gcgtccgcgg
     2281 ccgcgtccag aatgccaggg tcgagttctc tttgacgcgc gccgctgcgc aagcactgaa
     2341 cgagatcgtt gttaaccacg gtcgagtctc caaataaaac ggcacgcatc agtccacgca
     2401 cgccagatga aacgtatagg cgcgtccggt tcgccatggt cggggttaac agaaagttac
     2461 ccacttacca cacgctcagt ccgtgatgca gcgcacatag tggcgccgac gtaatccgca
     2521 gcgaaggtga gaacccggcg cgatcaacct tcgcgaccca accccggctt gagttccttt
     2581 cgtccacttg cgggagaccg acgatccatg caacctgccg cgggatagtc tgggcaagga
     2641 gcgaggagat gcctgcagac agagaaatta cagccgagta tctcatcatc ggcggaggcg
     2701 ccctcggcat ggccatcgcc gatacgttga tcggcgaaag cgacagcagc gtcatcatgg
     2761 tcgatcgacg acataagccc ggcgggcact ggcacgacgc ctaccccttc gtgcggttgc
     2821 acggcccctc tgccaactac ggcgtgaact cgcggcaact gggcagcgac cggatcgacg
     2881 aagccggcct gaatcggggc ctcttcgagc tggccacggg ctcagagatc cgaacctatt
     2941 tcgacgaagc gatgcgccac gggttgctcc cgaccggccg gatgcactat ctgcccatgc
     3001 acgaatacaa cggcgacggc caggccgtat ccctgatcag cggccaagct accaaggtga
     3061 gcgcgcgaaa gaaaatcgtc gacgcgacca ttgccgagac gcaggtgcct tcccgaacgt
     3121 ccccgaattt ttccgtcgct gaaggcgcca cgctgatacc tccgaacggc ctggcggaac
     3181 tcggcggctc ggcgagcacc tacgtgatca ttggagcggg aaagacggct atcgacgccg
     3241 tgacctggct gatggaaaac gacgtcgatc ccggccggct gaagtggatc cgccccaggg
     3301 acccctggtt gctcaacaga aagatgattc agccaagcta cgatttcttc gagaacacga
     3361 ttggctggct gatagccagc atggaagcag ccgcaaacgc cgacacggtc gacgacatct
     3421 tcctccatct ggaaagtgcc gggtgcatgc atcgcatcga cacatccgta attccaacga
     3481 tgtaccgttg cgcgatcgtc agcgagatgg agctcgatca attgaggctc gtgaaggacg
     3541 tcgtgaggat gggccatgtg cgagcgattg cagataaccg cgtcgttctc gacgacggcg
     3601 aactcgacct gtcgccgggg accctcttcg tcaactgcag cgcggacggc atccctcgca
     3661 agcccgtgca accgattttc caacccggga aaatcgtgcc tcaatacgtg cgcgcctgct
     3721 cgcccacgtt cagcggcgcg ctcgtcgcaa aggtcgagct ccttttcggc gacgacgaag
     3781 aaaagaacgc cctcaccagg cccacaccca ttccggattc cccggcagac tgggttcaga
     3841 tcgagctcaa tcaagcgctg aattccgccg cctggcgcga gaacaaggcg ttgtcgcagt
     3901 ggctggcagg ctcccgcctt gatcagttca cagcgatgat gttgcgcgca ttctccgagg
     3961 gcgacgaggc gaagataggg ttgctggaca ggtaccgcac ggccatccgg cccgcggtgg
     4021 agcgaatggg caagatggtt gcggccagca cgtcggcctg acggaacccg cctctattgc
     4081 cccgatattt attgccccga tattgccccg atccgtcgtc acctctcgtg tcttgaaagc
     4141 cacaacactc ggtttcccga ctcccgttca cgctttcttc aggattgcag gtttacactt
     4201 ccccgcgtta aaggcgccta cagccgcaga ggagcgattc atgaagataa gttgcctggg
     4261 aagaatgggg gccatttcaa tctgtgtcgc tgtcgggttg aacgcttgca ccaccaatcc
     4321 gtataccggg gaacgtgaag cgtccaatac tgccaagggc gcaggcatcg gcgcggccat
     4381 cggggccgtg gttggcgtgc tgagcggcga cgacgcggac gagcgtcgca agaatgcgct
     4441 tatcggcgcc ggtgtcggcg cgctcgcggg cggcgccgtg ggagcttaca tggacgccca
     4501 ggaagccaag ctccgggagc aactcgccaa caccggcgtc agcgtgagtc ggctcggcga
     4561 cgacatcgtg ctgaacatgc ccggcaacgt gacgttcgac gtg
//
LOCUS       DBNHOX010000308         2908 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_989716, whole genome shotgun sequence.
ACCESSION   DBNHOX010000308 DBNHOX010000000
VERSION     DBNHOX010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_989716"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="AC16UZ_06735"
     CDS             <1..452
                     /locus_tag="AC16UZ_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012271164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR6246417.1"
                     /translation="MSADAFHITSPAEDGAGAAACMQNALADAGLRSDQIDYINAHGT
                     STPLGDVAETVAIKRVFGDDTRVAVNSTKSMIGHLLGAAGSVEAIFTILAIRDQASPP
                     TINLDNPGEGCDLDYVPNVARQMEIRAGLSNSFGFGGTNGTLIFASV"
     gene            482..1468
                     /gene="mltG"
                     /locus_tag="AC16UZ_06740"
     CDS             482..1468
                     /gene="mltG"
                     /locus_tag="AC16UZ_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR6246418.1"
                     /translation="MRLFLVSFAALILLSGSAAFYVVRWNDAPLALTEPVVVVLEAGE
                     PFSKFAARISASGYLTHPRLWSRLARLRGLARQAKAGEYALQPGDTPERLLQRIVRGE
                     VVTYEVRLLEGWTVMQVLLALQENQVLEHELVGANVTTLLEALGLPGGHAEGLFYPDT
                     YQFVRGSSDRDILRRAYDRMQMVLEGAWDQRASGLPYRTPYEALIVASLVEKETGRDE
                     DREHISQVFVSRLNANMRLQTDPSVIYGLGERFDGNLKRVHLRESTPYNTYVIRGLPP
                     TPIALPGGKSIDAALHPGPGDYLYFVSRGDGTSQFSRSHAEHLAAVRKYQLK"
     gene            1465..2106
                     /gene="tmk"
                     /locus_tag="AC16UZ_06745"
     CDS             1465..2106
                     /gene="tmk"
                     /locus_tag="AC16UZ_06745"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR6246419.1"
                     /translation="MSVDSGSRGRFITLEGTEGVGKTSNLEHVCHLLQRAGYEVVATR
                     EPGGTPLAESIRSLLLEVREEPVDPTVELLMVFAARAQHVARVIEPALAAGRWVVCDR
                     FTDATFAYQGGGRGISMEDIATLESLVQGDLRPDLTLYLDVPVEVAAKRIAGRDHDRF
                     EREQMDFFERVRKTYLERAADEPQRVRVVDARQDLATVQAEIDRVVAEFLDRS"
     gene            2103..>2908
                     /locus_tag="AC16UZ_06750"
     CDS             2103..>2908
                     /locus_tag="AC16UZ_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246420.1"
                     /translation="MTALLPWHVTLADRIEGLMSAGRLPHGILITGTEGWGDSILAAW
                     LALRILAEDTDQNPRELAHPNLRWIVPDGAEIKVSAVRELGEFAFSTSQGGGAKVAVL
                     ENAHLLNRNAANALLKTLEEPPANTFLVLATSHPGRLLATIRSRCQRFVIQPDAQAAR
                     GWLQGRWSEAELASRLPETGGAPLLVDAALTEGSTALRECLEVALSSPRPLDSIAELL
                     ERDAGQVTSGWYRTLRDLIAGREEIGALRQTDSRALFEFADELLWVRHQLV"
BASE COUNT          503 a          909 c          972 g          524 t
ORIGIN      
        1 ggatgagcgc cgatgccttt cacatcacca gccccgcgga ggatggcgca ggcgccgctg
       61 cgtgcatgca gaacgcgctt gcagacgcgg gcctgcgttc ggatcagatc gactacataa
      121 acgctcacgg aacatccacg ccgctcggcg acgtggcgga gacggtggcc atcaaacgcg
      181 tcttcggaga cgacacccgg gtggccgtca attccaccaa gtccatgatc gggcatctgc
      241 ttggcgcggc gggcagcgtg gaagcgatct ttacgattct cgccatccgc gatcaggcgt
      301 ctccgccgac catcaacctg gacaatcctg gcgaaggctg cgatctggac tacgtcccga
      361 acgtggccag acagatggag atcagggctg gtctgagcaa ttcgttcgga ttcggcggaa
      421 ccaacgggac cttgatcttc gcatcggtct gaacgacgcg cctgaggtag aatccggcgc
      481 gatgaggctc ttcctggtca gcttcgccgc cttgatcctt ctctccgggt ccgcagcatt
      541 ctatgtggtt cggtggaatg atgccccgct cgccctgacc gagccggtgg tggtcgtgct
      601 cgaggcgggc gaaccgtttt ccaagttcgc agcccggata agcgcgtcgg ggtacctcac
      661 acacccaagg ctctggtccc ggttggctcg tctgcgcggt cttgccaggc aggccaaggc
      721 cggagagtac gcgttgcagc ctggggatac gcccgaacgg ctgctgcagc ggatcgtccg
      781 cggtgaggtc gttacttatg aagtccggct gctcgagggc tggaccgtga tgcaggtgct
      841 gctcgcgttg caggagaacc aggtgctcga gcacgaactc gtcggcgcga acgtcaccac
      901 gctgctggaa gccctgggac tgccaggagg tcatgcggag gggctcttct atccggacac
      961 ctatcagttc gtgcgcggtt cgtctgatcg ggacatcctg cgcagagcct acgaccggat
     1021 gcagatggtt ctcgagggcg cctgggacca gcgcgcgtcg ggccttccct accgaactcc
     1081 atacgaagcg ctcatcgtgg catccctcgt ggagaaggag acgggtcgcg acgaggatcg
     1141 cgagcacatc agccaggtgt tcgtcagccg actgaatgcc aacatgcgcc tgcagacgga
     1201 cccgtctgtg atttacggtc tgggcgagcg gttcgacgga aacctgaaac gggttcatct
     1261 gcgcgagtcg accccttaca acacatacgt gatcaggggg ctcccgccaa cgcccattgc
     1321 tcttcccggt ggcaaatcga tcgacgcggc gttgcatccg ggccccgggg attacctgta
     1381 tttcgtctcc cggggggacg gtaccagcca gttttcccga agccacgccg agcatctcgc
     1441 cgcggtgcgt aagtatcagc tgaaatgagc gtcgacagcg gcagcagggg tcggttcatc
     1501 accctcgaag ggactgaggg cgtcggcaag acgagcaatc tggagcacgt ctgccatctt
     1561 ctgcagcgtg ccggatacga agtcgtcgcg acccgtgagc ctggcggcac gccgctggcc
     1621 gagagcattc gatcgttgct gctcgaggtg cgcgaggagc cggtagatcc caccgtggag
     1681 cttctgatgg ttttcgccgc ccgcgcccag cacgtcgccc gcgtcatcga accggcgctg
     1741 gcggccgggc gctgggtggt gtgcgaccgg tttacggacg ccaccttcgc ttatcagggc
     1801 ggcgggagag gcatctcgat ggaagacatc gccaccctgg aatccctggt tcagggggat
     1861 ctgcgtcctg atctgacgct ctatctcgac gtgcccgtcg aggtggctgc caagcgtatt
     1921 gccgggcggg atcatgaccg cttcgagcgc gagcagatgg acttcttcga gcgcgtgagg
     1981 aaaacctacc tcgagcgggc agcggacgaa ccgcagcgcg tgcgggtcgt ggatgcccgg
     2041 caggacctcg cgacggtgca ggcggaaatc gaccgcgtcg tcgccgaatt cctggatcgc
     2101 tcatgacggc actactgccc tggcatgtga cgttggccga tcgtatcgag ggcctcatgt
     2161 ctgccggccg gcttccgcac ggcatcctca tcaccggcac cgaaggatgg ggcgacagta
     2221 ttctggcggc ctggctggca ctgcggattc ttgcagagga taccgatcag aacccccggg
     2281 agctggcgca tccgaatctg cgctggatcg ttcccgacgg tgcggagatc aaggtcagcg
     2341 cagtgagaga actcggcgag ttcgcttttt ccaccagcca gggcggcggt gccaaggtcg
     2401 ccgttctgga aaacgctcac ctgttgaatc gcaacgcggc gaacgcgctt ctgaagaccc
     2461 tggaggaacc gccggccaac acgtttctcg tcctggcgac gagccatcct ggacggttgc
     2521 tcgcgacgat ccgcagccgt tgtcagcggt tcgtcatcca gccggatgcg caggctgccc
     2581 gtggctggct gcagggtcgg tggagcgagg ccgagctggc ctcgcggctg ccggaaaccg
     2641 gcggcgcgcc gctgctcgtg gacgcggcgc tcacagaggg ctcgacagcc ctgcgcgaat
     2701 gcctggaggt ggcgctgtcc tcaccccggc ccttggacag catcgccgag ctgctcgagc
     2761 gggatgcagg ccaggtcacc agcggctggt accgcacgct acgcgacctg atcgccggcc
     2821 gggaggagat cggcgctttg cggcagacgg acagcagggc gctgttcgaa ttcgccgacg
     2881 agcttctatg ggtgcgccac cagctcgt
//
LOCUS       DBNHOX010000309         2561 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_89566, whole genome shotgun sequence.
ACCESSION   DBNHOX010000309 DBNHOX010000000
VERSION     DBNHOX010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_89566"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="AC16UZ_06755"
     CDS             complement(<1..1373)
                     /locus_tag="AC16UZ_06755"
                     /inference="COORDINATES: protein motif:HMM:NF023165.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HIR6246421.1"
                     /translation="MITGFHCSTSWVRQRARVVALLGLLTLLVAGNTARGEPAMEAVA
                     ADGSTYPVEPVALLDPAALEELVGPIALYPDDLLAIALPAATYPLQVVQASRFLQSRE
                     ENPSVQPAEGWDDSVVALLNYPEVVELLNEDLDWTWKLGEAVLAQQEDVLEAVAAFRE
                     RARLAGNLRSDDFQLVESADEGIRIRPRDPEVIYVPYYEPSRVTAYHSRPVYHYYPAR
                     YPVYYYPYAPGHRFASGFWGVTSAFSIGWTTRRLHVHYVTYRDHPYYGHHYYDRYFYR
                     RPPSFRVDDHWQWHGSFAGLGRHHAGNYWYPRAHRHGPSPRHHHRRSHHEPNQDEGTD
                     REPRRHRERERERAADRPGGSEEHLADRRDPRERLVPELGPRRAGRLLARGSVVNGIP
                     VPESSATLAPRPARPPAAADSPGSAQRRPGSPIEAQQAIARARSALGARSAARRGALR
                     AQRSAGSP"
     gene            complement(1547..>2561)
                     /locus_tag="AC16UZ_06760"
     CDS             complement(1547..>2561)
                     /locus_tag="AC16UZ_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR6246422.1"
                     /translation="DVANETENADPRSGYRLAEGWPHYPADMQFEMGSGVAVDEAGVI
                     YLFTRDIEHWAAHPLAMKDKMGKSSISMFDRAGNYLGKWGPSDERGFALGAHTLYIEK
                     DGMFWTTDRDGHTVRKYHPDGTLLLTLGEFGQWGAGTDHFNGPTAVLVQENGNIVVSD
                     GYWNSRLVWFSPEGEFIREVGTWGTGPAEFGTPHAIAQDAQGRILVADLAGGNFHPYM
                     TLPGQIAEHRTRQIPDAKSRIQILDPDGAYIGEWDHIMPLSLAVYGDHVYASDKMSNL
                     AILDAATGKVEERVDNIAIYIHQLAMDAHGDLYTASVYPEHAGEKRGPEGPSHRRWTR
                     DGA"
BASE COUNT          418 a          841 c          864 g          438 t
ORIGIN      
        1 ggagagccgg ccgacctctg cgctctcaac gccccgcgcc tggcggcgct tctggcgccg
       61 agagccgaac gggcacgcgc gatcgcctgc tgcgcctcaa tgggtgagcc gggccggcgc
      121 tgggccgacc ccggggaatc ggcagccgcg ggcggccgcg cgggtcgcgg cgccagcgtg
      181 gccgacgact cgggcaccgg aatgccgttc accaccgatc ccctcgccaa cagacggcca
      241 gcgcggcggg gtccaagctc cgggacgagc cgttcccgcg ggtcgcgccg gtcggcgagg
      301 tgctcctcgg aacccccggg gcggtcagcg gctctttccc gctcccgctc ccgatgtcgt
      361 cgaggctcgc gatccgtccc ctcatcctgg ttcggttcgt gatggcttcg tcggtgatgg
      421 tgccgcgggc tcgggccgtg acgatgtgca cgcggatacc agtagttgcc tgcatgatgg
      481 cgtcccagcc cggcaaagct gccgtgccac tgccagtggt cgtcgacccg gaaggacggt
      541 ggccgacggt agaagtatcg atcgtaataa tgatggccgt agtagggatg gtcccggtag
      601 gtcacgtaat gcacgtgaag ccgcctggtg gtccagccga tgctgaatgc cgaggtcacg
      661 ccccagaacc cgctcgcaaa tcgatgtccg ggcgcatagg ggtagtagta aacgggataa
      721 cgagccgggt agtagtgata caccgggcgg gagtggtagg cggtcaccct ggaaggttcg
      781 tagtagggaa cgtagatcac ctcgggatcc cgcggccgga tccggatacc ctcgtccgcg
      841 ctctccacca gctggaagtc gtcgctgcgc aggttgccgg caagccgggc gcgttcccga
      901 aacgccgcga ccgcctcaag cacatcctcc tgctgggcga gcacggcctc gccgagcttc
      961 caggtccaat ccagatcttc gttcagcagc tccaccacct cggggtagtt gaggagcgcc
     1021 accaccgagt cgtcccaccc ctcagccggc tgcacggaag ggttctcctc gcgcgattgc
     1081 agaaaccgag aggcctgcac gacctgcagc ggataggtcg cggcgggcag ggcaatagcc
     1141 agaaggtcgt cgggatagag ggcgatgggc ccgaccagtt cctccagggc cgccggatcc
     1201 aggagcgcta ccggctccac cgggtaggtg cttccgtccg cggccacggc ttccatggcc
     1261 ggctccccac gggccgtgtt gcctgccacg agcagggtga gcaacccgag caaggcgacc
     1321 acccgggccc gctgccggac ccaggacgtt gagcaatgga accctgtaat catccgatgc
     1381 ctctcagctt cctgatccgc gtggcgaggc agtccctggc gttctttcca ctcatatcgg
     1441 acgagactat ccccgtcaac ctgaacggga catgaagggc agccggtcaa ccagggggcg
     1501 gggcggacgg gcggtccgcg caggaaagga tcagattcct tcgggatcag gcgccgtcgc
     1561 gggtccagcg ccggtgggac ggaccctcgg ggccgcgctt ttctccggcg tgctccgggt
     1621 agacggatgc cgtgtagaga tcaccgtggg cgtccatcgc cagctgatgg atgtagatgg
     1681 cgatgttgtc gacgcgctct tccaccttgc cggtggccgc atccagaatc gcgaggttgc
     1741 tcatcttgtc gcttgcgtaa acgtgatcgc cgtagacggc gaggctcagc ggcatgatgt
     1801 gatcccactc gccgatgtac gcgccgtccg ggtcgaggat ctgaatacgg ctcttggcgt
     1861 cgggaatctg ccgggtccga tgctcggcga tctggccggg cagcgtcatg tagggatgaa
     1921 agttgccgcc ggcgaggtcg gccaccagta tccgaccctg ggcgtcctga gcgatggcgt
     1981 gaggcgtgcc gaactccgcc ggcccagtgc cccaggtgcc cacctcgcgg atgaactcgc
     2041 cctcgggcga aaaccacacc agccgggagt tccagtagcc gtccgacacc acgatgttgc
     2101 cgttttcctg cacgagcacg gctgtggggc cgttgaaatg gtcggtcccc gcaccccact
     2161 ggccgaactc gccgagggtc agcagcaggg ttccgtcggg atggtattta cgcaccgtgt
     2221 ggccgtcgcg gtccgtggtc cagaacatgc cgtccttttc gatgtagagc gtgtgggctc
     2281 cgagggcgaa gccacgctca tcggaggggc cccacttgcc gaggtagttt ccggcccgat
     2341 cgaacatgga gatgctgctc ttgcccatct tgtccttcat ggccagagga tgagcggccc
     2401 agtgctcgat gtcccgagtg aacagataga tcaccccggc ctcatccacc gccaccccgg
     2461 agcccatctc gaactgcatg tcggccgggt aatggggcca accttccgcc aggcggtaac
     2521 cgctgcgtgg atcggcgttt tcggtctcgt tggctacgtc g
//
LOCUS       DBNHOX010000310         2652 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_860036, whole genome shotgun sequence.
ACCESSION   DBNHOX010000310 DBNHOX010000000
VERSION     DBNHOX010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_860036"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="AC16UZ_06765"
     CDS             <1..276
                     /locus_tag="AC16UZ_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007975857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR6246423.1"
                     /translation="TTIAQMKMFLTRIGFGSTAVITGDITQIDLPKGQKSGLVNVRNV
                     LSGIDGISFTHFGSKDVVRHPLVQKIVDAYEAYQEPHASPDSDRDAG"
     gene            266..721
                     /gene="ybeY"
                     /locus_tag="AC16UZ_06770"
     CDS             266..721
                     /gene="ybeY"
                     /locus_tag="AC16UZ_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017903146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR6246424.1"
                     /translation="MPVEVQIASVQAGLPSVARIEGWASAALDELGDEGEPPELCIRV
                     VDGEESRELNARFRGHDKPTNVLSFPADVDLPEAKLLGDVVICAPVVGVEAAEQGKSP
                     EDHFAHMVVHGVLHLSGYDHEQAGEAELMEAIERRILERVGIADPYAER"
     gene            728..1563
                     /locus_tag="AC16UZ_06775"
                     /pseudo
     CDS             728..1563
                     /locus_tag="AC16UZ_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798742.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
     gene            1665..>2652
                     /gene="lnt"
                     /locus_tag="AC16UZ_06780"
     CDS             1665..>2652
                     /gene="lnt"
                     /locus_tag="AC16UZ_06780"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR6246425.1"
                     /translation="MLQSSDRGGLLLGWLFGLGKYAAGVSWIYVSINVYGNAPPPLAA
                     FLVALFAGGLALFPMVNAWVFQRLRTKGAPLVNAWLFACLFVAFEWLLTWFLTGFPWL
                     YPAYGHVETVLGGLAPAGGVMLVSLGLAGSACSVAAAHQCARRLPVLTAALAPWVVGA
                     VLGAVSWVEPGAVRTVALVQGNVDQAVKWLPENRRPIIDRYRGLSEDHWGVDVMVWPE
                     AAITVMAHDAGEVLGDLEREGERSGTAVVLGLPAVERLADDRIVLRNTAIALGDGSGR
                     YVKRRLVPFGEYVPLEGWLRGLIEFFDLPMSRAAPGDWRQPLLRVGQEQAALA"
BASE COUNT          427 a          815 c          911 g          499 t
ORIGIN      
        1 accaccatcg cccagatgaa gatgtttctc acgcgaatcg ggttcggctc cactgcggtg
       61 atcaccggcg acatcaccca gatcgacctg cccaaagggc agaagtcggg gctggtcaac
      121 gtgcgcaacg tgctcagcgg catcgacggc atcagcttca cccacttcgg ctccaaggac
      181 gtggtgcgcc accccctggt tcagaagatc gtcgacgctt acgaggcata tcaggagcct
      241 catgcaagcc cggacagcga ccgtgatgcc ggttgaagtg cagatcgcat ccgtccaggc
      301 ggggcttccg tccgtcgcgc gcatcgaggg ctgggcgtcc gccgcgctgg acgagctcgg
      361 ggacgaaggc gagccgccgg agctgtgtat ccgggtcgtc gacggagagg agtcgcggga
      421 gctcaatgcc cgtttccgcg gtcatgacaa accaaccaat gtgctgagct ttccggccga
      481 cgtcgatctg ccggaagcca agctgctcgg agacgtcgtc atctgcgcgc ccgtggtagg
      541 cgtcgaggcg gccgaacagg gcaagtcgcc ggaggatcac ttcgcgcaca tggtcgtgca
      601 cggggtgctt catctgagcg gctacgatca tgagcaggcg ggggaagcgg agctcatgga
      661 agcgatcgaa agacgaatac tggagcgggt cggcatagcc gacccctacg ccgagcgatg
      721 agcgaggtcg gaggatcgga gcagcagcgg cgttccttgc tggactggct cgcagacctt
      781 ttcaacgacg agcccacgga ccgttccgag ctgatggaga cgctgcgcga tgctgccgac
      841 cggcagatca tggatagcga ggcgctcaac atcattttcg gggcccggca ggtgtcggac
      901 atgcaggcgc gggacatcat gattccgcgg acccagctcg tgtatctgga ggactccaag
      961 gcccccgagg agctgctcgc caccatcatc gagtctcagc attcgcgctt tccggttgtc
     1021 ggcgatgatc tggacgacgt caaaggcatt ctgcacgcca aggatctgct gccgctggcg
     1081 ctctccagcg gctcgggcag caatggcttc gacatcaagg actgcatccg gccggcaacg
     1141 atcgttccgg aaagcaagcg gttgaacgtc ctgctgcagg agttcagaag cacccgcaac
     1201 catatggcgg tcgtggtgga cgagtacggt cacgtttccg gcgtggtgac catcgaggac
     1261 gtgctggagc agatcgtggg cgagatcgag gacgagcacg acgtcatcga cgacagcttc
     1321 gtcaagcagc tcgattcccg aacgttcaac gtgaaggcaa cgactaccat cgaggatttc
     1381 aacgagcact tcgacctcga gctcgacgac gacgagttcg acaccattgg cggcctcgtg
     1441 ctcaaggcgt tcggccacct gcccgatcgc ggagagtcgg tgcggatcgg agatctcgac
     1501 ttcaaagtgc tcaatgccga ttcgcgaaga gtcaggctgc tgcaggtcaa gctgcgcaaa
     1561 tgaagcccct cctcgcgctc gcctgcggcg cgctggtccc tctctctctt gcacccttcg
     1621 gctggtagcc gcttgggctc gtcgccgtag gtggctggtt tgctttgctg cagtcgagtg
     1681 atcggggcgg gctgcttctg ggctggctgt ttggtttagg caagtacgcg gccggcgtat
     1741 cctggatcta cgtcagcatc aacgtttacg gcaacgcgcc gccgccgctg gccgccttcc
     1801 tcgtcgctct gtttgccggt gggctcgcgc tgttccccat ggtaaatgcc tgggtgttcc
     1861 agcgcctgcg gaccaagggc gcgccgctcg tcaacgcctg gctgttcgcc tgcctgttcg
     1921 tggcgttcga gtggctgctc acctggtttc tgaccggctt cccctggctc tatcccgcct
     1981 atggtcacgt cgagacggtc ctcggcgggt tggcccccgc gggcggggtg atgctggtga
     2041 gcctcgggct tgccgggtcg gcctgctccg tcgccgcggc ccatcagtgc gcgcgacggc
     2101 tgcccgtgct cacggcggct ctggcgccct gggttgtcgg tgccgttctg ggcgcggttt
     2161 cctgggtcga gcccggggcc gttcgcaccg tagcgctcgt gcagggcaac gtggatcagg
     2221 ccgtgaagtg gctgcccgag aaccggcggc ccatcatcga tcgctaccgg gggctgtcgg
     2281 aggaccattg gggcgtggac gtcatggtct ggccggaagc cgccatcacg gtgatggccc
     2341 atgacgccgg ggaggttctg ggtgatctgg agcgcgaggg cgagcggtcg ggcaccgctg
     2401 tcgtgctggg gctgccggcg gtggagcggc ttgccgacga tcggatcgtc ctgcgcaaca
     2461 cggccattgc cttaggggat ggatcggggc gttacgtcaa gcggcggctc gttcccttcg
     2521 gcgagtacgt cccgttggag gggtggctgc gggggctcat cgagttcttc gatctgccca
     2581 tgtcgcgcgc tgcgcccggc gattggcgac agcctctgct cagggtcggt caggagcagg
     2641 cggcgctggc ca
//
LOCUS       DBNHOX010000311         1705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_346756, whole genome shotgun sequence.
ACCESSION   DBNHOX010000311 DBNHOX010000000
VERSION     DBNHOX010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_346756"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..562)
                     /locus_tag="AC16UZ_06785"
     CDS             complement(125..562)
                     /locus_tag="AC16UZ_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6246426.1"
                     /translation="MTANSPTGDYVQMDSVDWIPFPEGLCEGPITWKLLHVSPESGAW
                     TAIFDCPAGTSFAKHIHMGPGEYFLTKGRMNVRGGSAEGGATAVAPGYGYEECNAQHD
                     HTEFPVDSEFYMTFLGPLNFIDDDGNTIALVGCKEVLDAWNAT"
     gene            complement(727..>1705)
                     /locus_tag="AC16UZ_06790"
     CDS             complement(727..>1705)
                     /locus_tag="AC16UZ_06790"
                     /EC_number="1.1.1.1"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HIR6246427.1"
                     /translation="YTLDGLDSEGVFPSILGHEGAGVVVETGPGVDSVQPGDHVIPLY
                     TPECRQCKSCLSRKTNLCTAIRATQGQGLMPDGTSRFSFEGKPIHHYMGCSTFSNYTV
                     VPEIAVAKIRADAPFDKACYVGCGVTTGVGAVVNTAKVEPGANCIVFGLGGIGLNVIQ
                     GLRMVGASRIIGVDVNPRKEAWGRQFGMTHFVDPGTVDGDLVAHLVELTDGGADYTFD
                     CTGNTDVMRTALEACHRGWGTSVIIGVAEAGQEIATRPFQLVTGRVWKGTAFGGARGR
                     TDTPKVVDWYMDGKIEIDPMITHTLPLERINEAFDLMHRGESIRSVVVY"
BASE COUNT          274 a          550 c          555 g          326 t
ORIGIN      
        1 aagcctgacc taccggttct aattcggcgt aggatcggtc gtgagaatac gggcgctcgt
       61 gtaatcacac gagcgcccgg ctggtttcag cggtttgccc ggcgccgggg ccgggcaatg
      121 acgactaagt ggcgttccag gcgtccagaa cctctttgca ccccaccagc gcgatggtgt
      181 tgccatcgtc gtcgatgaag ttgagtggcc cgagaaaggt catgtagaac tcgctgtcca
      241 cggggaactc ggtgtggtcg tgctgagcgt tgcactcctc gtagccgtag ccgggcgcaa
      301 cggcagtggc accgccttcg gccgagcctc cgcgcacgtt catcctgccc ttggtgagga
      361 agtactcgcc ggggcccatg tgaatgtgct tggcgaacga cgtgcctgcg gggcagtcga
      421 agatggccgt ccacgcgccg gattccggtg agacgtggag cagtttccag gtgatggggc
      481 cttcgcaaag gccttccgga aaaggaatcc agtccacgga atccatctgg acgtaatccc
      541 cagtggggct gtttgctgtc atgtcgatcc tcccaacctg aacgcagagc ttctgcgttc
      601 tttgctgact cgtccgatga agactcggac accctccact gcagtttgtc ccgcgctcag
      661 gtgggcgtca atgctttagc accgcgttgg cggcgcccgt ctcccttcgc gatcagagct
      721 gtccggtcag taaacgacca cggagcggat gctttcaccc cggtgcatca ggtcgaacgc
      781 ctcgttgatc cgttccagcg gcagggtgtg ggtgatcatg ggatcgatct cgatcttgcc
      841 gtccatgtac cagtccacca ccttcggcgt gtcggtccgc cctctggcgc cgccgaaggc
      901 ggtgcctttc cagacccgcc cggtgaccag ctgaaacgga cgcgtggcga tctcctggcc
      961 cgcttcggcc accccgatga ttacgctggt tccccagccc cggtggcagg cttccagcgc
     1021 cgtgcgcatg acgtctgtgt tgcccgtgca gtcgaaggtg tagtccgcgc cgccgtcggt
     1081 gagctccacc aggtgggcca ccagatcgcc gtccaccgtc cccgggtcca cgaagtgcgt
     1141 catgccgaac tggcgtcccc acgcctcctt gcgcgggttg acgtccaccc cgatgatccg
     1201 gctggcgccc accatgcgca ggccctgaat cacgttgagg ccgatgccgc cgaggccgaa
     1261 gacgatgcag ttggcgccgg gctcgacctt ggccgtgttc accacggccc ccaccccggt
     1321 ggtgacgccg cagcccacgt agcaggcctt gtcgaagggg gcgtcggcgc ggatcttcgc
     1381 caccgcgatc tcggggacca ccgtgtagtt ggagaacgtc gagcagccca tgtagtgatg
     1441 gatgggcttg ccctcgaacg agaatcggga ggtgccgtcg ggcatcaggc cttggccttg
     1501 cgtggcgcgg atggccgtgc acagattcgt ctttcgggac aggcaggatt tgcactgccg
     1561 gcactcgggc gtgtacaggg gaatgacgtg atcgccgggc tgcaccgagt ctaccccggg
     1621 gcccgtctcg acgaccacgc cggcgccctc gtggccgagg atggacggaa agaccccttc
     1681 ggaatcgagc ccgtcgaggg tgtag
//
LOCUS       DBNHOX010000312         3574 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_154194, whole genome shotgun sequence.
ACCESSION   DBNHOX010000312 DBNHOX010000000
VERSION     DBNHOX010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_154194"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1634)
                     /gene="nadB"
                     /locus_tag="AC16UZ_06795"
     CDS             complement(15..1634)
                     /gene="nadB"
                     /locus_tag="AC16UZ_06795"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016568828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HIR6246428.1"
                     /translation="MGQRFSTDILVIGGGAAGLSAALHLAEHARVTVLCKGDVESAST
                     HWAQGGIAAVTDPDDTYEAHIQDTLNAGAGLCDHSVVAQTVERAPQVISQLVQWGIEF
                     DRSENEYHLTREGGHSHRRILHVADATGKALTQTLTDKVLDHPNIELFTDRVAIDLIN
                     LSKFGRHPNRCAGAYVLNRTTNHVEVFQAHFVILATGGASKVYLYTSNPDSSTGDGIA
                     MAWRAGCRVANMEFNQFHPTCLFHPHAKSFLISEAVRGEGGVLCLPSGERFMERFDPR
                     LELAPRDIVARAIDHEMKRLGLDCVYLDISHRPRSFIKSHFPTIAQRCADFGIDMANE
                     PIPVVPAAHYTCGGVVIDGNGASDVPGLYAVGEVSCSGLHGANRLASNSLLECLVFGE
                     AAARDIKRRLDEPRWDVNVPQWDESQVVDSDEDVIIAHNWEELRRFMWDYVGIVRTDK
                     RLQRAQRRIDLLKQEVHEYYSNYRVSNDLIELRNLIHVAELIVRSALVRKESRGLHYN
                     LDHPETMSRADDTILFPGSMDRLDVLQPVFS"
     gene            1733..2314
                     /gene="rpoE"
                     /locus_tag="AC16UZ_06800"
     CDS             1733..2314
                     /gene="rpoE"
                     /locus_tag="AC16UZ_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HIR6246429.1"
                     /translation="MAKDTDKELVRRVQKGDRYAFDLLFSRYQHKILNLVSRYLKDPQ
                     DVEDVTQEAFIKAFRALPRFRGESAFYTWLYRIAINTAKNHLVARGRRPPGVDVDMED
                     AEIMDGADALRVSESPEAVLERDELSAAIDVAISQLPDDLRSAVTLREFDGLSYEQIA
                     HIMDCPVGTVRSRIFRARESIDAHIEPLLPRRR"
     gene            2438..3007
                     /locus_tag="AC16UZ_06805"
     CDS             2438..3007
                     /locus_tag="AC16UZ_06805"
                     /inference="COORDINATES: protein motif:HMM:NF015810.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HIR6246430.1"
                     /translation="MSEKLRESLSATMDGEADEFELRRVLDEMQKDGGLREVWSRYHI
                     AGMLMRGERVHFSSDIRERVWSQLQLGTDDEAVESAALPAAQAGPADGPRSRLGRLAG
                     LAVAASVAFAAVIGVIGLWDEGAGEPPATAGTVTIEGPRVSNPAFTSEISPRDLQRAN
                     ASLMHHFQHQGMNQPGVSSFVKMVTYERR"
     gene            3004..>3574
                     /locus_tag="AC16UZ_06810"
     CDS             3004..>3574
                     /locus_tag="AC16UZ_06810"
                     /inference="COORDINATES: protein motif:HMM:NF015825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HIR6246431.1"
                     /translation="MRPARFILLVFGVLVSAISVADEDPHAWLADMNRAFSELSYDGI
                     FTYFSGDDLDTLRVVHMVVDEVQRERLVHLNGAPREIVRTGEEVACILMPGDELLELE
                     GSIPSGPFARAFVRRYDRISSSYGLSLYGEDRIAGRQAVRMAVSPLDDDRFGYRLWLD
                     KETRLLLRSELIDADGNRLEIFQFNQIVFG"
BASE COUNT          648 a         1048 c         1208 g          670 t
ORIGIN      
        1 cgagaccgga cggatcagct gaagacgggt tgaagcacgt ccagacggtc catcgatccc
       61 gggaacagga tggtgtcgtc cgcccgactc atcgtctcgg gatggtcgag gttgtagtgc
      121 aggcccctgg actcctttcg gaccagcgcc gagcgcacga tgagctcggc gacatggatc
      181 agatttctca actcgatcag gtcgttgctg acgcgatagt tgctgtagta ctcgtgcacc
      241 tcctgcttga ggaggtcgat ccgccgctgc gcgcgctgca ggcgcttgtc cgtcctgacg
      301 atgccgacgt agtcccacat gaagcgcctg agttcctccc agttgtgggc gatgatgacg
      361 tcctcgtcgg aatcgaccac ctggctctca tcccactgcg gcacgttgac gtcccaccgc
      421 ggctcgtcga ggcggcgctt gatgtcccgg gcggcggctt ccccgaaaac caggcattcg
      481 agcagcgagt tgctcgcaag ccggtttgct ccgtgcagcc cggagcagct cacctcgccc
      541 acggcgtata gcccgggcac gtcgctggcg ccgttgccat cgatgaccac gcccccgcag
      601 gtgtagtgag cggccggcac gacggggatg ggttcgtttg ccatgtcgat gccgaagtcg
      661 gcgcaacgct gggcgatggt aggaaagtgg ctctttatga acgagcgcgg ccggtgactg
      721 atgtccaggt agacgcagtc cagcccaagc cgcttcatct cgtggtcgat ggctctggcg
      781 acgatgtcgc gcggggcgag ttccagccgg ggatcgaaac gctccatgaa tctctccccg
      841 gagggcaggc acagcacgcc gccttcccca cggaccgcct cggagatgag gaacgatttg
      901 gcgtgggggt gaaacaggca ggtggggtgg aactggttga actccatgtt cgccacgcgg
      961 cacccggccc gccacgccat ggcgatgccg tcgcccgtgg agctgtccgg gttgctcgta
     1021 tacagataca ccttgctcgc gccgccggtc gccagaatca cgaagtgggc ctggaaaacc
     1081 tccacgtggt tggtggttcg gttgagcacg taggcgccgg cgcagcggtt tgggtggcgc
     1141 ccgaacttgc tcaggttgat caggtcgatg gcgacccgat ccgtgaagag ctcgatgttc
     1201 gggtggtcga ggaccttgtc ggtgagggtt tgggtgagcg ctttgccggt ggcgtcggcg
     1261 acatgaagaa tgcggcgatg agagtggcca ccttcgcggg tcaggtggta ctcgttctcg
     1321 ctgcgatcga actcgatgcc ccactgcacc agctggctga ttacctgggg cgcgcgctcc
     1381 actgtctgcg ccacgacgct gtgatcgcac agaccggcgc cggcgttcag cgtgtcctgt
     1441 atgtgggcct cgtaggtgtc gtccggatcc gtcaccgcgg ctatgccgcc ctgagcccag
     1501 tgagtggaag cgctctcgac gtcacccttg cacagaacgg taacacgagc atgctcggcg
     1561 aggtgcagag cggccgacag gccggccgcg ccgccgccga tgacgagaat gtctgtggag
     1621 aaccgctggc ccatgtccac cgcccggaat cagttagcca actagtataa acgggacgca
     1681 atcggcagaa tatcgggcct cttggggaac accggggtcg aaccgggtct aagtggcaaa
     1741 agataccgac aaggaactgg taaggagagt ccagaagggt gaccgctacg cgttcgatct
     1801 tttgttcagc cgctatcagc acaagatcct caacctcgtt tcccgctacc tcaaggatcc
     1861 ccaggatgtg gaggacgtta cacaggaagc tttcataaag gcgtttcggg ccttgccgcg
     1921 atttcgaggc gagagcgcgt tctatacctg gctgtaccgc atcgcgatca acaccgcgaa
     1981 gaaccacctc gtcgcacgcg ggcggcgccc gcccggagta gacgtggaca tggaagatgc
     2041 ggaaatcatg gacggtgccg acgccctgcg ggtgagcgaa agcccggaag cggtgctgga
     2101 acgggatgag ctttccgccg ccatcgatgt ggcgatatct caattgcccg acgacctgcg
     2161 cagcgcggta actcttcggg aattcgatgg tctaagctac gagcagatcg ctcacatcat
     2221 ggattgcccg gtgggtacgg tacgttcaag gatattcagg gccagagaat ccatcgatgc
     2281 tcacatcgag ccactgttgc cgcgcaggcg gtgacggcga ggttgctggg tcagcggttg
     2341 gtggtcgata cggctgcgcg gggagagacc gcgcaggaca gggtaaggat agggtaaaga
     2401 aggtgctcgt tgaagacgag agcaggggaa gcaggagatg tcggaaaaac tgagagagtc
     2461 gctttcggcg accatggacg gcgaggctga cgagttcgaa ctgcgtcgcg tcctcgacga
     2521 gatgcagaaa gatggtgggc tgcgtgaggt ttggagccgc taccacatcg caggaatgtt
     2581 gatgcggggc gaacgcgtgc atttcagcag cgatatccgg gaaagggtct ggagccagtt
     2641 gcagctcggt accgatgacg aagcggttga atcggccgcg ctgccggcag ctcaggccgg
     2701 gcccgcggat ggcccgcggt ccagactggg cagattggcc gggctcgcag tggcggcgtc
     2761 ggtcgccttt gcggcggtga tcggcgtgat cggtctgtgg gacgaaggag cgggcgagcc
     2821 cccggcgact gcgggcacgg tgaccatcga agggccccgg gtcagcaatc ctgcgtttac
     2881 cagcgagatc tcgccccgcg atctgcagcg agccaacgcg tccctgatgc atcactttca
     2941 gcatcagggg atgaaccagc ccggcgtctc ctcgttcgtg aagatggtga cctacgagcg
     3001 tcgatgagac ctgcacgctt cattctgctg gtgttcggcg tgttggtttc ggccatttcc
     3061 gtggccgacg aggatcccca cgcgtggctg gccgatatga accgtgcctt cagcgagctc
     3121 agctacgacg gcatattcac ctatttcagt ggcgatgatc tcgacaccct ccgggtggtg
     3181 cacatggtgg tcgacgaggt tcagcgggaa cggctcgtgc acctcaacgg agcgccgcgg
     3241 gagatcgtgc gtacgggtga ggaggtggcc tgcatcctga tgccgggtga cgagctgctg
     3301 gagctcgaag gcagcatccc gtccgggccc ttcgcgcgcg ccttcgtccg gcgctacgac
     3361 cgcatttcga gttcctatgg gctctcgctc tacggcgagg accgcatcgc ggggcgtcag
     3421 gcggttcgca tggccgtttc cccgctagac gacgatcgct tcggctatcg cctgtggctg
     3481 gacaaggaga cccggctgct gctgcgttcc gagctgatcg acgccgacgg caaccgcctg
     3541 gagatctttc agttcaacca gatcgtcttc ggcg
//
LOCUS       DBNHOX010000313         7469 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_796367, whole genome shotgun sequence.
ACCESSION   DBNHOX010000313 DBNHOX010000000
VERSION     DBNHOX010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7469)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_796367"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1357)
                     /locus_tag="AC16UZ_06815"
     CDS             complement(68..1357)
                     /locus_tag="AC16UZ_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR6246432.1"
                     /translation="MSLGDENAPLAERLRPNCLEEFVGQAHLLGAGKPLRTLAESGRL
                     HSMLLWGPPGTGKTTLAKLLAARAGARWISLSAVLSGVKDVRAAVAEAEALRGLGETT
                     VLFVDEVHRFNKAQQDAFLPHVEKGTVTFIGATTENPSFEVNAALLSRARVYVLKPLG
                     EEDLNRVIDRAAEQALNLPVTPDARALLTSLADGDARRMLNLLEVAAQLAEKEVDEAV
                     VTEAAGSRYRRFDKGGDAFYDQISALHKSVRGSAPDAALYWLCRMLDGGCDPLYVARR
                     VVRMATEDVGNADPRALQIALAAWDTYERLGSPEGELALAQAVLYLASVPKSNAAYVA
                     YGRAMGFVREGASEAVPMHLRNAPTRLMKDLGHGRDYRYAHDEPEHYAAGETYWPDGL
                     DPQAFYEPSDQGLEAKIRERLSRLRRLDQNTSGRAGR"
     gene            complement(1341..1970)
                     /gene="lolA"
                     /locus_tag="AC16UZ_06820"
     CDS             complement(1341..1970)
                     /gene="lolA"
                     /locus_tag="AC16UZ_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein periplasmic chaperone
                     LolA"
                     /protein_id="HIR6246433.1"
                     /translation="MKSWFRIATDPVVWLVAALSCASAAADPRQALSERLAAMDVFVA
                     EFSQEIRGSQGQVLERSTGRFFLDRPQFKWEVDHPYPQTVVTDGDRLKVYDADLEQLT
                     IRPLAEALKDTPVSLLTREGVALGNEFQVVEVTDELGASYILTPNAPDTLYAQIRLHF
                     SDNALAALAITDHLGQFTEILFSTSAEPPVIQSSEFQLVVPPGTDVIGG"
     gene            complement(1942..4194)
                     /locus_tag="AC16UZ_06825"
     CDS             complement(1942..4194)
                     /locus_tag="AC16UZ_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR6246434.1"
                     /translation="MTTSTIPVREIGLIGFTAAALYTMLAMVSYSPADPAFSFTGTGG
                     SVDNLVGRSGAWFADFTLYLFGRVAYILPVALVVIGVRLFRERSETFSWWLLALRCAG
                     WLAVLIFGCILARIHFAPAAALPGGAGGILGEALVALGLPVFGWIGLTLIALTAVMIG
                     AQAAAGFSWLSVAELTGRWVHAFTANAVLWLDRQLDRWRRFREARSGAKEMMRAREEA
                     LGDERAKRGSRAKPTIKPAAAQKASTVAVQEPLFESTPSGDLPGLDLFDERQPGDDRG
                     YSDESLEAISRLLELKLKDFGVEAEVVSVLPGPVVTRFEIQPAAGVKVQKISSLVKDL
                     ARSLAVISVRIVEVIPGKSVVGIEIPNEHREVVRLKEVFSSAVFQDSKSPLTLALGKD
                     IAGESVVADISRMPHLLVAGTTGSGKSVGVNAMLLSILCKATPEDVRMILVDPKMLEL
                     SVYEGIPHLLTPVVTDMKEAAHALNWCVAEMERRYKLMAALGVRNLAGYNRQIADAAK
                     KGKTISDPLWDADSEGEPAALGKLPLIVVFIDEFADMMMIVGKKVEQLIARIAQKARA
                     AGIHLVLASQRPSVDVITGLIKANIPSRISFQVSSKVDSRTILDQGGAEQLLGHGDML
                     YLPPGSGLPTRVHGAFVSDEEVHRVVSDWKQRGEPDYDNGVLSGGDDEPFLSIEPDSG
                     SAEQEDALYDEAVAFVLESRRASISSVQRKLRIGYNRAARLIEAMEAAGVVSAMNSNG
                     SREVLVPNRD"
     gene            4360..4659
                     /locus_tag="AC16UZ_06830"
     CDS             4360..4659
                     /locus_tag="AC16UZ_06830"
                     /inference="COORDINATES: protein motif:HMM:NF038106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4642 family protein"
                     /protein_id="HIR6246435.1"
                     /translation="MRPDKTKVVDEVWDDDRIEGFLHKAPLGDERSADYSALLYAYRS
                     MRPDDFATFIRLFVDAGRDVNAKSNGGETLLATIRDHRHGAPFRAILEKAGGSAS"
     gene            4678..5382
                     /gene="aat"
                     /locus_tag="AC16UZ_06835"
     CDS             4678..5382
                     /gene="aat"
                     /locus_tag="AC16UZ_06835"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011533432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HIR6246436.1"
                     /translation="MSGLSVLSSHRVGFPPPESALAEPNGLLAVGGSLTPEWLLAAYA
                     RGIFPWFDDDASPLLWWSPDPRAVLLPEELRVTRSLAKRIRNAGFNVTMDQAFADVVA
                     ACAAPRGAEAGTWITADMARAYAELHEMGFAHSVETRLDGELVGGLYGVSLGRMFFGE
                     SMFARQRDASKVALYHLTLRARDWNFSLIDCQIMNEHLRSLGAREMPRAEFLRLVHEN
                     RAEPTRRGSWADETAR"
     gene            5379..6089
                     /locus_tag="AC16UZ_06840"
     CDS             5379..6089
                     /locus_tag="AC16UZ_06840"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003108766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HIR6246437.1"
                     /translation="MKDETADKQFFITPAHPCSYLPKRNAQTLFLDPRETITPQTYQQ
                     LTEQGFRRSGGHLYRPHCKTCQACVPTRLPVAEFVARRSQRRTLARNTDLAVRVEPAA
                     YSPAHYSLYASYIAGRHGDGDMYPPSPDQFRSFLLSQWSDTLFVNSFLDGKLVAVAVT
                     DKLDDGLSAIYTFFDPALEQRSLGVQSILQQIELARELGLSYLYLGYWIRDAEKMRYK
                     TDYRPSELFVNGRWVRIT"
     gene            6171..6386
                     /gene="infA"
                     /locus_tag="AC16UZ_06845"
     CDS             6171..6386
                     /gene="infA"
                     /locus_tag="AC16UZ_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR6246438.1"
                     /translation="MAKEEQIEMEGTVIDTLPNTMFRVELDNGHVVTAHISGKMRKNY
                     IRILTGDKVKVELTPYDLSKGRITFRK"
     gene            complement(6390..>7469)
                     /locus_tag="AC16UZ_06850"
     CDS             complement(6390..>7469)
                     /locus_tag="AC16UZ_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012724940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6246439.1"
                     /translation="IDEAGAYQRLQPESRRRKLIGVREIEDIVAKIARIPPRNVSASD
                     KEALRNLDQDLKQVVFGQEPAMDALATAIKLSRAGLKVTEKPIGCFLFAGPTGVGKTE
                     ASRQLAKTLGLELIRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAVTKHPHSIV
                     LLDEIEKAHPEVFNLLLQVMDHGTLTDNNGRKADFRNVVLVMTTNAGAQDMSRPSIGF
                     TQQDHTSDAMEAIKKLFTPEFRNRLDGIIQFHSLPKEVIKTVVDKFLIELQAQLDDKK
                     VTLEVDDAAREWLVVEGYDEKMGARPMQRLIQERIKRQLAEDVLFGALSKNGGTVYVT
                     VKDGDLHLRCEAPEGSADKTPAATT"
BASE COUNT         1377 a         2511 c         2293 g         1288 t
ORIGIN      
        1 ccgatcagga accgccccac ggcgcccagc gcgccgccga gagcaaccca gaccagagcg
       61 ttcgcggtca ccttcccgcc cggccggacg tgttttgatc gagccggcgc aggcggctca
      121 gccgctcgcg gatcttcgct tccaggccct gatcggaagg ctcgtagaat gcctggggat
      181 ccaacccgtc gggccagtag gtctctccgg ctgcgtagtg ctcgggctcg tcgtgggcat
      241 aacggtagtc acggccgtga cccagatctt tcatcagtcg ggtgggggca ttgcgaaggt
      301 gcatgggcac cgcttcagac gccccctcgc gcacgaagcc catagcccga ccgtaagcga
      361 cgtaagcggc gttgctcttg ggcacgctgg cgagatacag caccgcctgg gcgagcgcca
      421 gctccccctc aggcgagccc aggcgttcat aggtatccca cgctgcaagg gcgatctgca
      481 gggcccgagg gtccgcattg ccgacgtcct ccgtcgccat gcgcaccacc ctgcgggcga
      541 cgtagagcgg atcgcaacca ccgtccagca tgcggcacag ccagtagagc gcggcgtccg
      601 gagcactgcc ccgcacggat ttgtgcagcg cggagatctg gtcgtagaag gcatcgcccc
      661 ccttgtcgaa ccgccggtag cggctgccgg cggcctccgt caccacagcc tcgtccacct
      721 ccttctcggc cagctgagcg gccacctcga gcaggttcaa catccgccgc gcgtcgccgt
      781 cggccagcga ggtcagaagc gcgcgagcat caggggtgac cggcaggttc agcgcctgct
      841 ccgccgcccg atcgatgacc cggttgagat cttcctcacc caaaggcttg agcacgtaaa
      901 cccttgcccg ggacaagagc gcagcattca cctcgaaaga tgggttctcc gtggtcgccc
      961 cgataaaggt cacggtgccc ttttccacat ggggcagaaa ggcatcctgc tgggccttgt
     1021 tgaaccggtg cacctcgtcg acgaacagca ccgtggtctc gcccaatccc cgcaaggctt
     1081 ccgcctcagc cacggcggcc cgaacgtctt tcaccccgct cagcacggca gagaggctga
     1141 tccatcgagc accggcccgg gcggccagca gcttcgccag ggtggtcttg ccggtacccg
     1201 gcggccccca gaggagcatg gaatgaaggc ggccggactc tgccagggtc cgcaggggct
     1261 ttccggcgcc cagaagatgc gcctgaccca cgaactcctc caggcagttc ggcctgaggc
     1321 gctcggctag cggtgcgttc tcatccccca atgacatcgg ttcccggcgg cacgacgagc
     1381 tgaaactcgc tcgattgtat caccgggggt tcggccgacg tgctgaagag gatctccgtg
     1441 aactgaccga gatgatccgt gatggccagg gcggccaaag cgttgtcgga gaaatgcagg
     1501 cgaatctgcg catacagggt gtcgggcgcg ttgggcgtga ggatgtagga agcaccaagc
     1561 tcgtcggtga cctccaccac ctgaaactcg ttgcctaggg ctacgccctc gcgggtaagc
     1621 agcgacaccg gcgtgtcctt cagcgcctcc gccagaggac ggatcgtcag ctgctcgaga
     1681 tccgcatcgt aaaccttcag ccgatctccg tcggtcacca ccgtctgcgg gtaagggtgg
     1741 tcgacttccc acttgaactg cggccggtcg agaaagaacc gtccggtgga gcgctccagc
     1801 acctggccct gagaaccgcg aatctcctgg ctgaactccg ccacgaacac gtccattgcg
     1861 gccagccgtt cggatagcgc ctgccggggg tcggccgccg ccgaggcgca ggatagcgcg
     1921 gcgacgagcc agacgacggg gtcagtcgcg attcggaacc aggacttcac gactgccgtt
     1981 gctgttcatg gcgcttacca cgccggctgc ttccatggct tctatgagcc gcgcggcacg
     2041 gttgtagccg atccggagct tgcgctgcac ggatgagatg gacgcgcgcc gcgactcgag
     2101 gacgaaggcc accgcctcgt cgtacagggc gtcctcctgc tccgctgacc cggaatccgg
     2161 ctcgatggag agaaaaggct cgtcgtcacc gccggacagt acgccgttgt cgtaatcggg
     2221 ttcgccccgc tgtttccagt cggagactac ccggtgcacc tcctcgtcgg agacgaacgc
     2281 accgtgcaca cgagtcggca gtccagagcc cggtggcagg tagagcatgt ccccatgacc
     2341 cagaagctgc tcggccccgc cctgatcgag gatcgtgcgc gagtccacct tggacgacac
     2401 ctgaaagctg atgcgcgaag gaatgttggc cttgatgaga cccgtgatca cgtccaccga
     2461 cggacgctgc gacgccagga ccagatggat cccggccgct cgcgccttct gggcgatgcg
     2521 ggcgatcagc tgctccacct tcttgccgac gatcatcatc atgtcggcga actcgtcgat
     2581 gaacacgacg atgagcggca gcttgccaag ggccgccggc tcgccttccg aatcggcgtc
     2641 ccacagcgga tccgagatcg tcttaccctt cttggcggcg tctgcaatct gacggttgta
     2701 gccggccagg ttgcgcacgc cgagcgccgc catgagcttg tagcgtcgtt ccatctcggc
     2761 cacgcaccag ttgagcgcgt gcgccgcctc cttcatgtcc gtcaccaccg gcgtgaggag
     2821 gtgaggaatc ccctcgtaca ccgacagctc cagcatcttg ggatccacca ggatcatccg
     2881 gacgtcttcc ggggtggcct tgcagaggat gctcagcagc atggcgttga ccccgacgga
     2941 cttacccgaa cccgtggtgc cggccaccag caggtgaggc atgcgggaga tgtcggccac
     3001 caccgactcg ccggcgatgt ccttgcccag ggccagggtg agcggcgatt tcgagtcctg
     3061 aaagacggcc gagctgaaaa cctccttcaa ccgcaccacc tcgcggtgct cgttggggat
     3121 ctcgatgccc accacggatt tgccgggaat cacctccacg atccgcacgc tgatcaccgc
     3181 cagggaacgg gccaggtcct tcaccaggga gctgattttc tggaccttga cccccgcagc
     3241 cggctgtatc tcgaaccgcg tcaccacggg gccgggcagc acggagacga cctcggcctc
     3301 tacgccgaag tccttgagct tgagctcgag cagtcgcgag atcgcctcga gggattcatc
     3361 cgagtacccg cgatcgtccc cgggctgacg ctcgtcgaaa aggtccaatc ccggcagatc
     3421 gccgctgggc gtggattcga aaagcggttc ctgcacggca accgtcgacg ctttctgcgc
     3481 ggcggcgggt ttgatggtcg gcttcgcgcg gctcccgcgc ttcgcgcgct cgtcgcccag
     3541 ggcttcctcc cgggcacgca tcatctcctt agcgcccgag cgggcttcgc gaaaacgacg
     3601 ccagcggtcc agctgtcgat cgagccagag cacggcgttt gcggtaaagg cgtgcaccca
     3661 ccttcccgtc agctccgcga cgctcagcca ggagaaaccg gcggccgcct gggcgccgat
     3721 catcaccgcg gtcagcgcga tgagcgtaag cccgatccag ccgaacacgg gcagcccgag
     3781 ggcgaccagg gcttccccca agatgccacc cgcaccgcca ggcaaggccg ccgccggagc
     3841 aaagtgaatg cgcgcgagga tgcagccgaa gatcagaacc gccagccagc cggcgcagcg
     3901 cagggcgagc agccaccacg aaaacgtttc gctgcgttcc ctgaagagcc gaacgccaat
     3961 gaccaccagc gcaacgggca ggatgtaggc aactcttccg aacagataca aggtgaagtc
     4021 ggcgaaccag gcgccactgc gacccaccag attgtcgacg ctcccgccgg tgcccgtgaa
     4081 cgagaacgcg ggatcagcgg gtgagtagga aaccatggcg agcatggtgt acagggcggc
     4141 ggccgtgaat ccaatcaggc cgatttcccg gacaggaatc gtcgatgtgg tcacgaattt
     4201 ctccttaaat tcggcgcgaa gtgtagcggc aaaaccatct gcgaacaaat ctctatttaa
     4261 ttcaattcgt tacccgaact acctcaatca gaacttgccg acttaccggc agtccaacca
     4321 atcgttatca tagccaccga acctgaacac gggccaaaca tgcgaccaga caaaacgaaa
     4381 gtcgtcgacg aggtgtggga tgacgaccgc atcgaagggt tcctgcacaa agcgccgctg
     4441 ggcgatgagc gcagcgccga ctactcagcg ctgctgtacg cctaccgcag catgcggccc
     4501 gacgatttcg ccacgttcat tcgcttgttc gttgacgccg gacgggacgt caacgcgaaa
     4561 agcaacggcg gagaaaccct gcttgccacc atccgcgacc accgccatgg cgcgcctttc
     4621 cgcgccattc tggaaaaggc cggcggtagc gcgagctgaa gcgaagcgcc tagaacgatg
     4681 tccgggctaa gcgtgctatc aagccaccgt gtggggtttc ccccaccaga gtccgccctc
     4741 gccgaaccca acggcctgct ggccgtgggc ggcagcctga cgccggaatg gctgctggca
     4801 gcttacgcgc gcggcatctt tccctggttc gacgacgatg catcacccct tctctggtgg
     4861 agccccgacc cccgggccgt gctcttgccg gaggaactgc gggtaacgcg cagccttgcc
     4921 aaacgaattc gcaatgcagg tttcaatgtg accatggacc aggcgtttgc ggatgtggtc
     4981 gcagcatgcg ccgcgccccg cggcgcggag gccggcacct ggatcaccgc ggacatggcc
     5041 cgcgcctacg ccgaacttca cgagatgggc tttgcccact cggtggagac ccggctggac
     5101 ggcgagctgg tgggggggct ctacggcgtt tctctggggc gcatgttttt cggagagtcc
     5161 atgtttgcca ggcagcggga cgcttcgaag gtcgccctgt accatctgac cctgcgcgcc
     5221 cgggactgga atttctctct catcgactgt cagatcatga acgagcacct gcgctcccta
     5281 ggcgcccggg aaatgccccg agccgagttc ctccggctag tccatgaaaa ccgggccgag
     5341 cctacgcgcc gcggatcgtg ggcggacgag acggcccgat gaaagacgag accgccgaca
     5401 agcagttctt catcacgccc gcccacccct gcagctacct gcctaagcgc aacgcccaga
     5461 cgctctttct cgatccccgg gagacgatca cgccgcaaac ctaccagcag ctcaccgagc
     5521 agggttttcg gcgcagtggc ggccatcttt accggcctca ctgcaagacc tgccaggcct
     5581 gcgtgcccac gcgcctcccc gtcgccgagt tcgttgcgcg ccggagccag aggcgaaccc
     5641 tggcacgcaa tacggacctg gccgttcggg tggaacccgc cgcgtacagc cccgcccatt
     5701 actcgcttta cgccagctac atcgcaggcc gccatgggga tggcgacatg tacccaccct
     5761 cccccgacca gttccgttct tttctcttga gtcagtggtc ggacaccctg ttcgtcaaca
     5821 gcttcttgga tggcaagctc gttgccgtgg cggtcacgga caagctggat gatgggcttt
     5881 ctgccatcta caccttcttc gatccggcgc tagagcagcg cagcctgggc gtgcagagca
     5941 tcctccagca gatcgagctc gcccgggaac tcgggctgtc ctatctgtat ctgggctact
     6001 ggatccgcga cgcggaaaaa atgcgctaca agaccgacta ccgccccagc gagctgttcg
     6061 tgaacggacg gtgggtgcgt atcacctgag ctttgcatgt gcccggacca tgaggcagaa
     6121 tgccgcacgc aaaattcggg ccgcccgtaa acggacggcg tcaggagtat atggctaaag
     6181 aagaacagat cgagatggag ggaacggtga tcgacaccct ccccaacacc atgttccgag
     6241 ttgagctgga caacgggcac gtggtcaccg cgcacatctc cggcaagatg cgcaaaaatt
     6301 acattcgcat cctcaccggc gacaaggtca aggtagagct taccccctac gacctcagca
     6361 aaggccgcat caccttccgc aaatagactt caagtggtcg ccgccggcgt cttgtcggcg
     6421 gacccttcgg gcgcttcgca gcgcaggtgc aggtcgccgt ctttgaccgt gacgtaaacg
     6481 gtcccgccat tcttcgacag cgcaccgaac agcacgtctt cggccagctg gcgcttgatg
     6541 cgctcctgaa tcaaccgctg catgggccgg gcgcccatct tctcgtcgta gccctcgacg
     6601 acgagccact ccctcgcagc atcgtccacc tccagggtga ctttcttgtc gtccagctgg
     6661 gcctggagct cgatgaggaa cttgtccacc accgtcttga tgacctcttt gggcagggag
     6721 tggaactgaa tgatgccgtc gagccgattg cggaattccg gcgtgaacag cttcttgatg
     6781 gcctccatcg cgtccgaggt gtggtcctgc tgagtgaagc cgatggaggg ccggctcatg
     6841 tcctgggccc ccgcgttggt ggtcatgacc agcaccacgt tgcggaaatc ggccttccgg
     6901 ccgttgttgt cggtgagcgt tccgtggtcc atgacctgca gcagcaggtt gaagacttcc
     6961 ggatgggcct tctcgatctc gtccagcagc acgatgctgt ggggatgctt ggtaaccgcc
     7021 tcggtaagca ggccgccctg gtcgaagccc acgtatcccg gcggcgcccc gatgagtctg
     7081 gagaccgtgt gccgctccat gtactccgac atgtcgaagc ggataagctc cagccccagc
     7141 gttttcgcca gctgtcggct ggcctcggtc ttgccgacgc cggtcggacc ggcgaacagg
     7201 aagcagccga tcggcttctc cgtcaccttg agcccggcgc gcgagagctt gatcgcggtc
     7261 gccagcgcgt ccatcgccgg ttcctggccg aagacgacct gcttgaggtc ctggtcgagg
     7321 ttgcgcagcg cctccttgtc cgaggccgag acgttgcgcg gcggaatgcg cgcgatcttg
     7381 gccacgatat cctcgatctc gcgcacaccg atcagtttgc gccggcgcga ttccggctgc
     7441 aggcgctggt aggcacccgc ctcatcgat
//
LOCUS       DBNHOX010000314         1742 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_346963, whole genome shotgun sequence.
ACCESSION   DBNHOX010000314 DBNHOX010000000
VERSION     DBNHOX010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_346963"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..1680)
                     /gene="tnpC"
                     /locus_tag="AC16UZ_06855"
     CDS             complement(253..1680)
                     /gene="tnpC"
                     /locus_tag="AC16UZ_06855"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HIR6246440.1"
                     /translation="MAFKSSAIRPGSNASPPSSDASSSCNRLLLSQAGKDQPPPTRRL
                     RTQGRRDIGELEHLPLIEQVYELTDELCRCPSCQAEREKIGSAISYTVEYIPGSLVRI
                     KHMQHKYACRRCEQDGHNPNITLAPKTNGSPIDKGMPGPGLLAYVATAKFGDYLPLHR
                     LQTIFAREGFELDRSTMCVWMADVARLVGPLYDRMVQRVLQSHVLATDDTVMPMQHPG
                     RTRKSRMWIYQGDESQPYNVFDFTVSHSRDGPARFLTDFRNTLLADAYGGYDGIVLTR
                     ELPRAGCWAHARRKFTDCQAAAPQVTQAILHLIKHLFSLESRVKNVDAEERLRRRQAE
                     AVPLLESLHALLLEEKAKLLPKHPLSEAIQYTLNQWPALTLFASDPAVPIHNNLAEQE
                     MKRIALLRKNALFVATPRGGQTAAILSSLTSTCRRHDINPQAYLTQLLANLPDTPIDR
                     VDEWLPDEWKRRMADRPLQTRLEQS"
BASE COUNT          289 a          520 c          595 g          338 t
ORIGIN      
        1 tgcctcgcaa agaatcccga tgatcggatt caaaccgccc atgacgcggc gctcaatttg
       61 cgctggatac aggaaggtgg atctcggctt ggggtttcgg cggccactag ttcgcacagg
      121 cgcagcaaag aaagaattaa gtggacacta gcgataatcg tctgtgttct ttcgtgtccg
      181 cttaatgaat cgcaccccca agttgcgcga ggcagcgagc ctgccagaac gtgaaggcgg
      241 cgcttttttt gatcaggatt gttcgaggcg ggtttgcagc ggtcgatcgg ccatccgccg
      301 cttccactca tccggtagcc actcgtcgac gcggtcgatc ggcgtatcgg gcaggttggc
      361 caggagctgg gtgaggtagg cctgcgggtt gatgtcatgc cggcggcagg tgctggtcag
      421 gctgctcagg atggcggccg tctggccgcc gcggggtgtg gccacgaaca acgcgttctt
      481 tcgcaggagg gcgattcgct tcatctcctg ctcggccagg ttgttgtgga tcggcacggc
      541 cggatcgctc gcgaacagcg tcaacgccgg ccactggttc agcgtgtact ggatcgcctc
      601 gctgagcgga tgcttcggca gcagcttggc cttctcttcg agcagcagcg catgcagtga
      661 ctccagcaac ggcacggcct cggcctgtcg ccggcgcaga cgttcctcgg cgtctacatt
      721 cttgaccctc gactccagcg agaacagatg cttgatcaga tgcaggatcg cctgcgtcac
      781 ctgcggggcg gctgcctgac aatcggtgaa cttgcggcgg gcatgggccc agcatccggc
      841 gcggggaagc tcccgcgtca gtacgatccc gtcataaccg ccataggcgt ccgccagcaa
      901 agtgttccgg aagtccgtga ggaaccgagc cggcccgtca cgcgagtggc tgacggtgaa
      961 gtcgaagacg ttgtacggct gcgactcgtc gccctggtag atccacatcc gcgacttcct
     1021 ggtcctgccc ggatgctgca tcggcatcac ggtgtcgtcc gtcgccagaa cgtgcgattg
     1081 cagcacccgc tgcaccattc ggtcgtagag cggccccacc agccgggcca cgtccgccat
     1141 ccacacgcac atcgtcgatc ggtcgagctc gaagccctcc cgggcgaaga tggtctgcag
     1201 tcggtgcaag ggcagatagt cgccgaactt cgccgtggcc acgtaggcca gcaggcccgg
     1261 gcccggcatg cccttgtcga tcggggagcc gttcgtcttg ggcgccaggg tgatgttggg
     1321 gttgtggccg tcctgctcgc agcggcggca ggcgtacttg tgctgcatgt gcttgatgcg
     1381 gaccagcgag cccgggatgt actcgacggt gtagctgatg gccgagccga tcttctcccg
     1441 ctcggcctgg cagctcgggc aacggcacag ttcgtccgtc aattcgtaca cctgctcaat
     1501 gagcggcagg tgctcgagtt cgccgatgtc ccgccggcct tgcgtgcgca gccggcgcgt
     1561 gggcggaggc tgatccttgc cggcctgcga aaggaggagc ctattgcatg acgagctggc
     1621 gtcggagctt ggcggggagg cgttggagcc gggtcggatc gcagaggact tgaatgccat
     1681 gagcgaggag gtgtggcggg actttggact ggtgcacggc tggtatgagg cggtcaagca
     1741 ag
//
LOCUS       DBNHOX010000315         4283 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1182593, whole genome shotgun sequence.
ACCESSION   DBNHOX010000315 DBNHOX010000000
VERSION     DBNHOX010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1182593"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="AC16UZ_06860"
     CDS             complement(<1..1213)
                     /locus_tag="AC16UZ_06860"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR6246441.1"
                     /translation="MTRFIFVTGGVVSSLGKGIASSSLAALLEARQLNVNVLKMDPYI
                     NVDPGTMSPFQHGEVFVTADGAETDLDLGNYERFTHARMSRRNNFTTGSVYAEVLRRE
                     RRGDYLGGTVQVIPHITDEIKRRINEAGQGFDVLIVECGGTVGDIESQPFLEAIRQMR
                     LEVGAANAIIIHLVLVPFVPSAGEIKTKPTQHSVKELRSIGLQPDLLVCRSDRPLPAS
                     AAGKIALFTNVEERAVISLPDAESIYQVPQALRDGGIDDIILDKMGLDCPPADLSEWG
                     RVVDAQLHPERAVRIAIVGKYLDLLDAYASLIEAITHAGIQTRIRVDIDFIDAEDLER
                     SGTGALERVNAILVPGGFGQRGFEGKIAAARYARENGLPYLGICYGLHAAVIDYARHV
                     CGLDGAHSTEID"
     gene            complement(1295..2572)
                     /gene="tilS"
                     /locus_tag="AC16UZ_06865"
     CDS             complement(1295..2572)
                     /gene="tilS"
                     /locus_tag="AC16UZ_06865"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR6246442.1"
                     /translation="MDAGQLVEEALRRALRGAVAPGRVFVGYSGGMDSTVLLHAAIAA
                     SPVPVTAVHANHGLDPQAGRWQAHCERVCAQWGVGLESREVSVGHGNVEAAARAARYG
                     FFESVLSASDLLLLAHHQDDQAETVMLRMVQGRGLIGIPSARRLGAGELRRPFLELPR
                     QVLAEYADGLALDWVEDPGNADEGLDRNYLRSRVLPSLRRRWPGFAGQMQVLLDQRAS
                     TEQMLLARVDGDFESIAVGELLCGDGGSGAELLGIWLSSLGAALPSRGALASFVAQLG
                     SAADRQPELKLAQGSLRRYRGRVYRVADPPALAPSYELIPPGELRLPHGTVTVCEAHG
                     GGFQLHGPLKVMFRGPEGAGHIRCRGHRRSIKKLLQDAGLPPWKRLTYPLLEDADGVA
                     AVPGIAERDPDPDPDPPSGGTRWVARWTAHSIR"
     gene            complement(2577..>4283)
                     /gene="dnaE"
                     /locus_tag="AC16UZ_06870"
     CDS             complement(2577..>4283)
                     /gene="dnaE"
                     /locus_tag="AC16UZ_06870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR6246443.1"
                     /translation="FLRTAETTGVFQLESSGMKDLIRRLKPDSIDDIIALVALFRPGP
                     LQSGAVDDYIDRKHGQEPITYPHPEMKAVLQNTYGVMLYQEQVMQTAQVLAGFSLGQA
                     DLLRRAMGKKKPEEMAKVREQFLSGCSERGVDAALAGQIFDLMEKFSGYAFNKSHSAT
                     YALVSFQTAWLKTHHPAEFMAATLSVDMQNTDKVVTLVDETRNMGLPLSPPSVNRSEY
                     RFSAREGAVVYGLGAVRGVGEGPVQALVEARASGGAFESLPDFCRRVDSRKANRRVLE
                     ALIRAGAMDDFAEPGEAVDCLRARLMAELGDALHGAEQAARDVALGMSDMFGGLEEPT
                     LGNGNGRGAPPALGKRERLEWEKEALGLYLSGHPIEDYLGEIREFCGRPIADLEAGKR
                     TQVVAGLVFSARTMRGRRGEMGFVVLDDRSKRIEVSLFSEVFQAGKEKVAKDAVLVVE
                     GEVQPDDYTGELKMRADNVYTIEEARRRFARELVIDCCDASPTADFSSRLKTCLEPYR
                     DGSQGCSIAVMYQSPATSGPGARGRIVLGRDWAVIPCDDLLASLRSEFGDDRVAFRYA
                     GN"
BASE COUNT          751 a         1462 c         1367 g          703 t
ORIGIN      
        1 cgtctatctc ggtggaatga gcaccgtcca atccgcagac gtggcgggcg tagtcgatca
       61 cggcggcatg tagaccgtag cagattccca gatagggcag accgttctcg cgcgcgtagc
      121 gggcggctgc gatcttgccc tcgaagcccc gctggccgaa accgcccgga acgaggatgg
      181 cgttcacgcg ttccagggcc ccggtgccgg aacgctcgag gtcctcggca tcgatgaagt
      241 cgatgtccac ccggatcctc gtctgaatac cggcgtgagt gatggcctcg atgagcgagg
      301 cgtacgcgtc cagcaggtcc aggtatttgc cgacgatggc tatcctcaca gcccgctcgg
      361 gatggagctg ggcatccacc acacgccccc actcgctgag gtcggcgggc gggcagtcca
      421 agcccatctt gtcgaggatg atgtcgtcga tacccccgtc gcgcagggcc tggggcacct
      481 gatagatcga ctcggcgtcc ggcagcgaga tgaccgcgcg ttcctccacg ttcgtgaata
      541 gggcaatctt gccggcggca gacgccggca ggggtcggtc ggaacggcag accaggagat
      601 cgggttgcag gccgatggat ctgagctcct tcaccgagtg ctgggtgggc ttggtcttga
      661 tctccccggc ggacggcacg aaggggacga gcaccagatg aatgatgatg gcgttcgccg
      721 cgcccacttc cagccgcatc tggcgaatgg cctccaggaa cggctgcgac tcgatgtcac
      781 caaccgtccc cccacactct acgatcagca catcgaagcc ctgcccggct tcgttgatcc
      841 tgcgtttgat ctcgtcggtg atgtgcggga tcacctgaac cgttccgccc aggtagtctc
      901 cacggcgctc ccgccgcagc acctcggcgt agacgctgcc agtggtgaag ttgttccgcc
      961 gcgacatgcg ggcgtgggtg aaacgctcgt agttgccgag atccagatcc gtttccgccc
     1021 cgtcggcggt cacgaagact tcaccgtgct gaaacgggct catcgtcccg ggatccacat
     1081 tgatgtaggg atccatcttc aacacgttta cgttgagttg ccttgcctcg aggagcgctg
     1141 cgagggacga cgaggcgata cctttaccca gagaggatac gacaccgcca gtaacgaaaa
     1201 tgaatcgtgt catctattcc atcttgaagc ccgaggtccg cgattgctgc gaacgcaaga
     1261 tggccttgaa agataacaaa agcccccggc aacctcaacg aatcgaatgc gccgtccatc
     1321 gggcgaccca gcgcgtcccc ccggacggcg ggtccgggtc cgggtccggg tcccgctccg
     1381 caatgcccgg aaccgcggct accccgtctg cgtcctcgag cagcggatag gtgagccttt
     1441 tccagggcgg aagacccgca tcctggagca gtttcttgat ggagcggcga tggccgcggc
     1501 agcggatgtg tcctgctcct tcagggccgc ggaacatcac cttgagcggg ccgtgtaact
     1561 gaaagccccc gccgtgcgcc tcgcacacgg tgacggtgcc gtggggcagc cgcagctccc
     1621 ccggcgggat cagctcgtag ctcggcgcca gagcgggcgg atccgcgacc cgatagacgc
     1681 ggccccggta gcggcgcaga gacccctgcg ccagcttgag ctccggttgc cggtcggcgg
     1741 cgcttccgag ctgcgccacg aacgaggcga gggcgcctcg ggaggggagc gcggccccga
     1801 gagagctcag ccagatgccc aacagctccg ccccggagcc gccgtccccg caaaggagct
     1861 cgccgacggc aatggactcg aagtcgccgt ccacccttgc cagcagcatc tgctcggtgc
     1921 tcgcccgctg atccagcaga acctgcatct gcccggcaaa ccccggccag cgccggcgca
     1981 ggctcggcag gacgcgactg cgcagatagt tccggtccag accttcgtcg gcattgccgg
     2041 gatcctctac ccaatcgagg gcgagcccgt cggcatactc cgccagcacc tgccgcggca
     2101 gttccagaaa tggtcgccgc agctcgccag cgcccagccg acgggcagac gggataccaa
     2161 tcagcccccg gccctgaacc attcgcagca tcacggtctc cgcctggtcg tcctggtgat
     2221 gagccagcag cagcaagtcg gatgcggaga gaaccgattc gaagaagccg taacgggcgg
     2281 cccgggcagc ggcttccacg ttgccgtggc ctaccgacac ttcgcgggat tcaagaccca
     2341 ccccccactg cgcgcagacc cgctcgcaat gtgcctgcca tcgcccggcc tggggatcca
     2401 ggccgtgatt ggcatgcaca gcggtgacgg gcactggaga cgcggcgatc gccgcgtgca
     2461 gcagaaccgt ggagtccatg ccgccgctgt agccgacgaa gactcggccc ggtgcgacgg
     2521 caccgcgcaa tgcccttcgc agcgcctctt cgaccagctg cccggcgtcc acgggatcag
     2581 ttcccggcgt atctgaaagc cacgcgatcg tcgccaaatt ccgagcggag gctcgcaaga
     2641 agatcatcgc aggggatgac ggcccagtcg cgaccaagca cgatgcggcc ccgggccccg
     2701 ggcccggagg tcgccggcga ctggtacatg accgctatgg agcatccctg ggaaccatcg
     2761 cgataaggct ccaggcaggt cttcagacgg gaggagaaat cggctgtcgg ggacgcgtcg
     2821 cagcagtcga tgacgagctc gcgggcgaag cgccgccgtg cctcctcgat cgtgtagacg
     2881 ttgtcggccc gcattttgag ctctccggtg tagtcgtccg gctgaacctc cccctccacg
     2941 acgagcacgg cgtccttggc gaccttctcc ttgcccgcct ggaagacctc cgagaacagc
     3001 gagacttcga tccgcttgct gcgatcgtcc agaaccacga aacccatctc gccccggcgg
     3061 ccgcgcatcg tgcgcgcgga aaaaaccagt ccggcgacga cctgggtgcg cttgccggcc
     3121 tcgaggtccg cgatggggcg gccgcagaac tccctgatct ccccaaggta gtcttcgatg
     3181 gggtggccgc tgaggtagag gccgagcgct tccttctccc actccaggcg ctcccgcttg
     3241 ccgagcgctg gcggcgcccc acggccgttg ccgttgccga gggtgggctc ctccagcccg
     3301 ccgaacatgt cggacatgcc cagagcgaca tcccgcgcgg cctgctcggc cccgtgcaac
     3361 gcgtcgccaa gctcggccat gaggcgcgcc cgcaagcagt ctacggcttc tccgggctcg
     3421 gcaaagtcgt ccatggcccc cgcgcggatc agcgcttcca gaacccgccg gttggctttg
     3481 cgagagtcga cgcggcggca gaagtcggga aggctttcga acgcgccgcc cgacgcgcgc
     3541 gcctcgacga gtgcctgcac ggggccctcg cccacgcccc gcacggcgcc aagaccgtaa
     3601 actacggcgc cctcccttgc cgagaaccgg tactcgctgc gattgacgct cggcgggctg
     3661 agcggcagcc ccatgtttct cgtctcgtcc accagagtca ccaccttgtc ggtgttctgc
     3721 atgtccacag acagggtggc cgccatgaac tccgccggat ggtgggtctt cagccacgcg
     3781 gtctgaaagg aaacgagggc gtaggtcgcg ctgtgcgact tgttgaaggc gtaaccggag
     3841 aacttctcca taaggtcgaa gatctggccg gcgagagcgg catcaacgcc acgctcgctg
     3901 cagccggaga gaaactgctc gcgcaccttg gccatctctt caggcttctt cttccccatg
     3961 gcgcgccgca acaggtcggc ctgaccgagg ctgaacccgg cgagcacctg ggcggtctgc
     4021 atgacctgct cctgatagag catcacgccg taggtgttct gcagcacggc tttcatctcg
     4081 gggtgcggat acgttatggg ttcctgacca tgcttgcgat cgatgtagtc gtccaccgca
     4141 ccggactgga gcggaccggg ccggaacagg gccaccagcg cgatgatgtc gtcgatgctg
     4201 tccggcttca gccgcctgat cagatccttc atcccggagg attccagctg aaacaccccg
     4261 gtggtttcgg ccgtacgcag gaa
//
LOCUS       DBNHOX010000316        20267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1696875, whole genome shotgun sequence.
ACCESSION   DBNHOX010000316 DBNHOX010000000
VERSION     DBNHOX010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 20267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20267
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1696875"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1629)
                     /locus_tag="AC16UZ_06875"
     CDS             complement(196..1629)
                     /locus_tag="AC16UZ_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR6246444.1"
                     /translation="MSDAIAPFVNELWDRSIIPTLSDYVRIPNKSPAFDADWEAHGYM
                     QAAVELLRGWCSVPDIGGLTTEIVTIPGRTPVLFCEVPATAPDAGTVLLYGHYDKQPE
                     FDGWEPGLGPWEPVLRDGKLYGRGGADDGYALFGSLAAIAALQAEGIPHGRCVLLIEG
                     CEESGSFDLPAYVELLEERIGQPDLVICLDAECGNYEQLWTTTSLRGMLPGVLTVKVL
                     SEGQHSGAAGGIVPSSFRILRALYDRVEDADTGSLHDCLYVPIPDDARRQLADVAQVL
                     GATTIDRFPWLDGVTPDGDDLQDLLLANAWEPSLATVGLAGAPDVGNAGNTLRPGTSA
                     KLVFRLPPTLDADEAARTIKAELERSPPNGAHVRFELETPQTGWCAPPVAPWLESALM
                     RASQQYFGKPAMYMGMGGTIPFMKMLGDSFPGVQFVVTGVLGPRSNAHGPNEFLEIAT
                     GKRITACVAAVLAEHARRHARRQARQN"
     gene            complement(1626..3473)
                     /locus_tag="AC16UZ_06880"
     CDS             complement(1626..3473)
                     /locus_tag="AC16UZ_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6246445.1"
                     /translation="MPMVLATVLALLVPSLSFAEPDPELETITVTAHRIRTDRPVLPV
                     LTRTTGTGPAVGADALRSLPSLAISQAGSLGSVTQVRVRGSEANHLMVLMDGVELADP
                     ATDASFNFANLSLTGTTRVELLAGAHSAIWGSDAVAGVLNFITKPTERRRRLSLEGGS
                     FDTRQAALQLADTTGSYHYNLALSEFRTDGTNIATTGEEDDGFDQSSYRLSGGYRSES
                     WTIEALVHHLKSDADFDPIPFPAFQPRDGDRVSKHDEYLGSVAATWHGDERFEQQIRL
                     SYFDTDNRSFADGARESRTAAARFKATSLTRLRASSRHAVTLMLEHEKERFEQRGAPS
                     IFGDPNQNQSVRTNSLALEYLWAPLDDLSLALSARTDDNSDFENKESYRAAARYRLTG
                     AVELWANWGTGIKNPSFVERFGFTPDTFLGNPDLDPETNRHLSAGAVIGHRQWTLRAT
                     AFRDRLEDEINGFYFDGAAGAFTSINEPGTSKRDGVEVETTLAIPTGVLSMGASWLDA
                     AEPDAAREIRRPQWQAYATVSHERGPFSAELSGYYVGNREDLDFSGFPAERVRLGDYV
                     LLRAHLAYRLGRHLKLALRGENLLDESYQDQLGYASPGRAVYLQVAIDL"
     regulatory      complement(3514..3723)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3726..4868)
                     /locus_tag="AC16UZ_06885"
     CDS             complement(3726..4868)
                     /locus_tag="AC16UZ_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6246446.1"
                     /translation="MGAGIGGLTLAAALGEAGFRNVTVYDRGEPGSPEPSDLVLSPNG
                     TRVLRALHLGPFLSRAHKPDAYVQRSWRSGFVLAHRPLGAFMEARYGAPEVVVARQAL
                     LEALATRCAHHGVAVRTGQPCARIAQDARSVTLTFGSQEICHDVVIGCDGEASAVRAG
                     MGYAETTQTTQDVVWTGTCPGTDLPIGLRANAVTTWLGPRAYLTQWPSADGCAARFLA
                     FARNPGAGAKLAGAFRNWHEDVFRLVDSATGVTRSAPVEREPIAQWYDRRVALLGDAC
                     HPVTPHLQQGASLAMEDAWVLSRMLERWEDEPAKGFPDYERYRKVRLGRFRQGQRHHL
                     AELTTDPGGKAWRRNLRLSLTSRFLPEIAMQRMDWLYGYDCIKGFE"
     gene            complement(4888..6285)
                     /gene="cysS"
                     /locus_tag="AC16UZ_06890"
     CDS             complement(4888..6285)
                     /gene="cysS"
                     /locus_tag="AC16UZ_06890"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR6246447.1"
                     /translation="MDIRLHNTKSGRKEAFDPVDPQRITMYVCGPTVYGLVHIGNGRP
                     AVVFDVLYRLLRAAHPEVVYARNITDIDDKINAAARDNDEPIQALADRFSSAYREDVA
                     ALGVLEPTVEPRATHHIAEIIAMIETLIAKGNAYEAQGHVLFHVPSDPEYGSLSHRSL
                     EDMLDGARVEVAPYKQDPKDFVLWKPSTTDLPGWDSPWGRGRPGWHIECSAMISKHLG
                     QTIDIHGGGSDLAFPHHENEAAQSRCANDHPEYVRYWLHNGMLTMGQEKMSKSVGNIV
                     TIRDLLDRFPGEVLRYALLSGQYRSQLAWSEDLLDQAGSSLERLYQALRDAGVEDETS
                     SDYRDLPAEEYPTVVVAALADDLNTPQALAAMHQLASAIHRTSDASEKVELARRLLAG
                     GWLLGILIESAKAHFTAGRDIDAAEIDRLIEERNAARKAKDFARADEIREELTDLGIE
                     LEDTRDGTRWKLRDG"
     gene            complement(6304..6705)
                     /gene="hisI"
                     /locus_tag="AC16UZ_06895"
     CDS             complement(6304..6705)
                     /gene="hisI"
                     /locus_tag="AC16UZ_06895"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015637550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HIR6246448.1"
                     /translation="MFEYLEDAELGHEVALDALLDAVKFDSDGLVPVIAQDADSRDVL
                     MLAWMNREAVERTLKDGYACYWSRSRGAFWRKGESSGHLQALKEMRFDCDGDAILLIV
                     DQTGPACHTNRPSCFYLSVSDGTARVSSEPV"
     gene            complement(6707..8071)
                     /locus_tag="AC16UZ_06900"
     CDS             complement(6707..8071)
                     /locus_tag="AC16UZ_06900"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013079.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HIR6246449.1"
                     /translation="MAEALIVSSDAAATPLRLIRTSDFEAFASELSEAERSWIRRAQF
                     AAKSGEFAWLPGADGHPAELVAGWDGRDDLASLGGLPTRIPEGVYELKTEVSDLQLLG
                     WALGSYQFVRYRERDRAPAQLLIPARNDADLVANMDAATTLVRDLINIPAGDMLPSHL
                     ADEAIQVADANQANCRVLVGEQLLEGGFDAIHAVGRAAADAPRLIDITWGDPDHPLIV
                     LVGKGVCFDSGGLDIKPAASMRLMKKDMGGAANALGLAQLIMASNLPVRLRVLIAAVE
                     NAVGANAYRPGDVVPTYKGISVEIDNTDAEGRMVMCDCLALGAEDEPALMVDFATLTG
                     SARSAVGAEIAAMFCNDDEVAAGLSAHGDRWDDPVWRLPLHKPYAYLLDSKVADTVNS
                     ASSPFAGSITAALFLEKFVGSAPWVHFDIMAFNTRPRPGRPEGAEAMAIRAVFGYLQE
                     RFGG"
     gene            8140..8700
                     /locus_tag="AC16UZ_06905"
     CDS             8140..8700
                     /locus_tag="AC16UZ_06905"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6246450.1"
                     /translation="MLTEVTTHHLELTDPDQFKPAATGPAYALKQVQRPTAELNRFLY
                     STVGAAWFWYERLSWSRARWLEFLDRPEVVTWVAYVDGSPAGYFELEDQPGGSVEICY
                     FGLLPAYIGQGMGGALLTDAIRAAWARGASRVWLHTCSLDHPFALANYLARGFSLFDI
                     VTTTKNLPDPMPEPRPGDAEAGRSSD"
     gene            complement(8692..9684)
                     /gene="lipA"
                     /locus_tag="AC16UZ_06910"
     CDS             complement(8692..9684)
                     /gene="lipA"
                     /locus_tag="AC16UZ_06910"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR6246451.1"
                     /translation="MSKPVVSGKKFRTPEGFTAIKDGMKANGQAEPATGRKPPWLRVR
                     LGGGEKYQAVHRTVATHRLATVCEESHCPNIGECWNNGTATIMLMGSVCTRACRFCAV
                     DTGNPGGRLDPDEPANAAESVRLMGLRYVVLTSVDRDDLADGGAGHYAKCIQAIKAVN
                     PETAVEALTPDFSGSAEAVAVVVGSGLDVFAQNLETVERLTGDVRDPRAGYDQTLGVL
                     AEAKQVRRDLLTKSSLMLGLGEQDDEICQAMDDLREHGVDILTLGQYLRPTRNHLAVA
                     RWVHPDEFERYREWGLERGFTEVASGPLVRSSYRADRILEGNNLGLGRIPVKSI"
     gene            9733..11169
                     /locus_tag="AC16UZ_06915"
     CDS             9733..11169
                     /locus_tag="AC16UZ_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6246452.1"
                     /translation="MQERPAVTRRVRWLYGAGAAAYGVKENGFSYFLLFYYNQVLDLP
                     GAYAGTAILIAMIFDAISDPLVGVWSDNTRSRWGRRHPFMYSAALPVAVIYFFLWNPP
                     ELTEFQLFVYLTVGCVLVRFFVTLYEIPSSAIVAELTDDYDERTRLLSYRYMMGWFGG
                     LGMAVLNWGLFMVVWGEAGETTFRVYGAVGAVIMFVAIVGSSLGLHRYIPYLHPPPER
                     TSYRVRQLTGDLIETISNRNFAALFFAGLFAAVGAGVATNFDTYIITHFWEFEAEQWR
                     WVIISLFVSALIPMYLAPLVTRRWDKKRSALGIYAFQIVFAALPILLRLAGWFPDNDS
                     PWTYPLIWAHAVVNVVVIVMFGVIQSSMLADVVEHSQASTGRREEGLFFAARSFAQKA
                     TSGVGAFIAGLALDWIAFPRGATPGTVPDAVIWDLGFIYGPALMIFYVLALVSIGFYR
                     ISRQGHDQRVGALRAGATAPSGSMEESR"
     gene            11166..11888
                     /locus_tag="AC16UZ_06920"
     CDS             11166..11888
                     /locus_tag="AC16UZ_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6246453.1"
                     /translation="MNDRPVIVTGGAGGIGSAIVARLVADGHPVAVLDLHPASATPSG
                     SLFSYPCDIADLDAVERAVAEFEAAVGAPFGLVNNAGWDQALPFLETGPDLWEKVIDV
                     NLRGPVNVTYAVLRRLAGAGRGRVVSIASDAGRVGSSGEAVYSACKGGIIAFMKTLAR
                     EHARQGITFNSVCPGPTDTPLLDSFDESGRLAQALERAIPMRRLGRPEDFPGIVAFLL
                     SDEASFITGQTVSVSGGLSMQG"
     gene            complement(11890..13767)
                     /locus_tag="AC16UZ_06925"
     CDS             complement(11890..13767)
                     /locus_tag="AC16UZ_06925"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6246454.1"
                     /translation="MTAASQPGAPERRNYSMFDAEISGAVLNLALLRRLLKWLRPYRV
                     TFGISAVLVLLASTLQVLMPIIISLVVIDHLIRGETDSLAPDLGMVELTEWLADILAL
                     HPLMAACLLYAGLQIAWAAAGHAHRMTLISSVINGLRDLRLDLFRHLETRPSSFYDRV
                     AVGRVMTRVTNDIEALYELLRGLGSLIGEFMPFFVALAIMLAIDVELTLLLLLALPVM
                     GSVTFYFRRATRRLFRMVRMSLSALNQNMQENLAGLQVVQLSERQQYNLDRYTKINEE
                     NRGHELRTTRFETLYGAFNDSMAHLALGVIIWYAGGAAVQEEMTLGAVILFTRFIDML
                     FHPIVVLGEQTNILFRAMASGERIFQALDWDEKIHEPADPVTLPPRLQGEVTFRHLNF
                     AYDPDVQILHDVSFEIGAGEKLAIVGPTGSGKSTLIRLLGRFYEFDDGQIFLDGIDLN
                     RIHTRDLRQRIGVVLQDFHIFSGTVFDNIALGDPRITRERAEEAARRVNAHGFISDLP
                     RGYDTELSERGQNLSQGQRQLLAFARVLAADPEILVLDEATASIDTETELLIQDALRK
                     LTAGRTSIIIAHRLQTIQEADRVLVLQGGRVVELGTHEELIASEGLYHTLHSLQFQDE
                     G"
     gene            complement(13764..15524)
                     /locus_tag="AC16UZ_06930"
     CDS             complement(13764..15524)
                     /locus_tag="AC16UZ_06930"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6246455.1"
                     /translation="MIRSLIRLKSFLAPYRLRLGAGVGAFGIARFFEAMVPIFVAIGI
                     DRMSAGNADMTTPLLGIAGAVIGRYVVVTYARYSVRCVGQHVAFDLRQALYESLQQQG
                     VRFFTRYTIGDMMTRAVADIGLIQRLIAMGTILMVILVYATLVGFGFMLYLSPSLTLL
                     LLPPLPLVFFYASWSARQMGVASRDVQNRLSDLGAHAQENLSGIRTIQAMVQEENEIR
                     RFGRTNQQYANAFYRQSQINSLMAAWMPSLAAICSVTILGYGGYLVLDGAMTVGELVA
                     FFMYVNMVVQPFRVAGFIVNLFQRAAVASDRLFEVITLSPEIADDPSGTTPATIRGDV
                     EIRHLTFAYDEGIRPALDDVSLTVARGESVTIMGRVGSGKTTLLKQLVRLLDTPPGAV
                     LIDGHDVRDYPLAQLRSQVAMVPQDAFLFGEPLGDNLTYDDPGRAVEKIWEAATSADL
                     KETIEEFPEQLDTIVGERGVTLSGGQKQRATLARGLIRGAPVLILDDCFSSVDTETEE
                     HILRELRRLRRRQTTILVSHRVSTARHSDRIVVMDAGRIVESGTHEELLARAGQYAEL
                     ERIQREGAVDTDYDLIGATT"
     gene            complement(15549..16940)
                     /gene="asnS"
                     /locus_tag="AC16UZ_06935"
     CDS             complement(15549..16940)
                     /gene="asnS"
                     /locus_tag="AC16UZ_06935"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIR6246456.1"
                     /translation="MTDTIKDILAHRLPVGAEVTIEGWVRSRRTSKGGFSFVHVSDGS
                     CFDTIQAVADSQLANYEHDVEHLGTGCSVRIRGTVVESQGRGQDREIQATEVVVVGWV
                     DDPETYPIAKKRHTFEYLREVAHLRPRTNTFGAIARVRNAIAQAVHGYFNDNGFVWVN
                     TPIITASDCEGAGQLFRVSTLDIANRALTRGDSPFDDDFFGRETFLTVSGQLNLESYC
                     LALSKVYTFGPTFRAENSNTSRHLAEFWMIEPEIAFADLNANAELAEDFLKAVCRRVL
                     DACPDDMSFFADRVDEQAISRLEHVIATPFERIDYTDAVSILEKAGQNFEYPVQWGLD
                     LQSEHERYLTEVHVGGPVVVVNYPKTIKAFYMRLNDDDATVAAMDVLVPGVGEIIGGS
                     QREERLDVLDARMAEQDIGEDLWWYRDLRRYGTAPHAGFGLGLERLVLYMTGMDNIRD
                     AIPFPRVPHNASF"
     gene            17038..17814
                     /locus_tag="AC16UZ_06940"
     CDS             17038..17814
                     /locus_tag="AC16UZ_06940"
                     /inference="COORDINATES: protein motif:HMM:NF022665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HIR6246457.1"
                     /translation="MRNLIIVIALVLVLLGGLWLASDRSAGDREVPLPGAEPVPVPGP
                     RPAEPARRPQPMPVAEPAIVLPDLDASDPFLRERLAGVELPQNWIDRDDLVRRLAVVI
                     ENAARGEYPRRQLGFLAPSGRFRVVERQGRLFVDPDNYRRFDGYLDVLEGLDPRTAAE
                     LLSLIEPLLAVAMGELGSDGSVSDQIDAAVAEVMAVPDVVGEVELAQPKVLYEYADPA
                     LEALSPLQKQVLRMGPENAARLKRYVAEMAVALQPDPSDG"
     gene            complement(17840..19048)
                     /locus_tag="AC16UZ_06945"
     CDS             complement(17840..19048)
                     /locus_tag="AC16UZ_06945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6246458.1"
                     /translation="MTSRTARILHSIDEIPAEDWNRCAMPGGEIPHDPFLRHEFLAAL
                     EHSGSAAARTGWQPFHLVLEEADRILGVVPMYVKNHSQGEYVFDYAWADAWHRAGGDY
                     YPKLQVSVPFTPATGRRVLAAGQDEVSRPEIERALLGACAQVAAEIKVSSLHVTFLPE
                     AQWQIAGELGFLRRMDQQFHWHNAGYGSFEEFLGDLSSKKRKNLRRERRDALATGIRI
                     EWLTGSDIQESHWDAFYRFYMDTGSRKWGSPYLTRQFFSLVTETMAEDVLLIMCSRDG
                     RYIAGALNFIGGEALFGRNWGCIEHHPFLHFEACYYQAIDFAIERGLKRVEAGAQGPH
                     KVARGYLPCPTYSAHWIADAGFRNAVARYLDEEREHVREDIEYIEERSPFKSTVSLDK
                     FHNTRTLDIG"
     gene            complement(19115..19576)
                     /locus_tag="AC16UZ_06950"
     CDS             complement(19115..19576)
                     /locus_tag="AC16UZ_06950"
                     /inference="COORDINATES: protein motif:HMM:NF015325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR6246459.1"
                     /translation="MKHLLIVYHTQTGRTGQLAAAVHRGATNELVSDVDARLLTAQNA
                     GADDLLWADGLLLGTPENFGYMSGGMKDFLDRTFYPVEGKILSLPYAIFISAGNDGSG
                     ALRAIRRIANGYPFKEVQEPVIARGEITNDHIDRCEELGLTLAAGLEAGIV"
     gene            19645..>20267
                     /locus_tag="AC16UZ_06955"
     CDS             19645..>20267
                     /locus_tag="AC16UZ_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR6246460.1"
                     /translation="MTQLDDITQARQRICGQIELTACSRSATLSAMTGADVHLKFENL
                     QFTASFKERGALNRLLQLTGEQQRFGVVAMSAGNHAQALAYHGKRLGVPTCIVMPRFT
                     PNAKVEQTRVHGAEVLLHGAEFDETLAFTEALAEERGLTLIHPFDDPAVIAGQGTLGL
                     ELVEQVPGLEAVLVPVGGGGLIAGVATAVKSVKPEVELLGVQVERFAS"
BASE COUNT         3216 a         6466 c         6922 g         3663 t
ORIGIN      
        1 gctgcaatca tcacaagggc agccggctgg ccgaggagtg cgggatggaa ctgctggcgc
       61 tgccctacgt tccgaacttt gccgagtacc tggccctgat caacagcggc cgtatcctcg
      121 gcgaccagat ggactttctg cgaaagagct tcggctcgaa cagccggctg ctgcaatagg
      181 cgtcggggcg tccggctagt tctgccgcgc ctgccgccgc gcatgccgcc gcgcatgttc
      241 ggccagcacg gcggcaacgc aggcggtaat gcgcttgccc gtggcgattt ctagaaactc
      301 gttcggcccg tgggcgtttg agcgtggccc gagaacgccg gtcacgacga actgcacgcc
      361 cgggaagctg tcgcccagca tcttcatgaa cgggatggtg ccgcccatgc ccatgtacat
      421 ggccggcttg ccgaagtact gctgggatgc gcgcatgagc gcgctttcca gccacggcgc
      481 gaccggcggc gcgcaccatc cagtttgcgg agtctcgagc tcgaagcgga cgtgggcgcc
      541 gttgggcggg ctccgctcca gttccgcctt gatggtccgc gcggcctcgt ctgcatccag
      601 ggttggcggc agccggaaga ccagcttggc gctggtgccc ggtctgaggg tgttgcccgc
      661 gttccccacg tccggagcgc cggcaaggcc cacggtggcg aggctaggct cccatgcatt
      721 ggccagcagc aggtcctgca ggtcgtcgcc gtcgggagtg acgccgtcca gccaggggaa
      781 gcggtcgatg gtcgtggcac cgagcacctg ggcgacgtct gcgagctgac ggcgcgcatc
      841 gtcggggatg ggcacataca ggcaatcgtg cagggaaccg gtgtcggcat cctccacccg
      901 atcgtagagc gcgcgcagga tccggaagct cgagggaacg atgccgccgg cggctccgga
      961 gtgctgcccc tcgctgagca ccttcacggt cagcacgccg gggagcatgc cgcgcagcga
     1021 ggtggtggtc cacagctgct cgtagttgcc gcactcggcg tcgaggcaga tgacgagatc
     1081 gggctggccg attcgctcct cgagcagttc cacgtaggcg ggcagatcga agctgccgct
     1141 ttcctcgcag ccttcgatga gcagcacgca tcggccgtgg ggaatgcctt ccgcctgcag
     1201 ggcggcaatg gcggccagcg agccgaacag ggcgtagccg tcgtcggcgc cgccgcgccc
     1261 gtacagcttg ccgtctctga gcaccggctc ccaagggccg aggccgggct cccagccgtc
     1321 aaactccggc tgcttgtcgt agtgaccgta cagcaggacg gtcccggcgt cgggcgccgt
     1381 ggccggcacc tcgcagaaca acaccggcgt gcggccgggg atcgtgacga tttcggtggt
     1441 gaggccgccg atgtcgggca cggagcacca ccctcgcagg agctcgacgg cggcctgcat
     1501 gtagccgtgg gcttcccagt cggcgtcgaa agccggcgac ttgttgggaa tacgaacgta
     1561 gtcgctcagc gtcgggatga tgcttcgatc ccacagctcg ttcacgaacg gggcgatggc
     1621 gtcgctcata ggtctatggc cacttgcagg tacacggcac gtcctggaga tgcataacct
     1681 agttggtcct gatagctctc gtccagaagg ttctcgccgc gaagcgcgag cttcagatgc
     1741 cggccgaggc gataagccag atgggcgcgc agcagcacgt agtcgcccag ccgaacccgc
     1801 tcggcgggga agccgctgaa gtcgagatcc tcccggttgc ccacgtagta gcccgacagc
     1861 tcggccgaga acggcccacg ttcgtggctc acggtggcgt aagcctgcca ctgcggcctg
     1921 cgaatctctc tcgctgcgtc cggctcggca gcatccagcc agctcgcgcc catgctcaag
     1981 acgcccgtgg gtatcgccag cgtggtctct acctctacgc cgtcgcgttt gctggtgccc
     2041 ggctcgttga tggaggtgaa cgcgccggcg gcgccgtcga agtagaagcc gtttatctcg
     2101 tcttccaggc gatcgcggaa ggcggtggcg cgcagggtcc attgccggtg cccgatcacc
     2161 gcccccgccg acagatgcct gttggtctcg gggtccagat ccggattgcc gagaaacgtg
     2221 tccggggtga acccgaagcg ttccacgaag ctcgggttct tgatgccggt tccccagttg
     2281 gcccagagtt ccactgcccc cgtcaggcga taacgggcag ccgcgcggta gctctccttg
     2341 ttctcgaagt cggagttgtc gtcggtgcgt gcggacagcg ccagggagag atcgtccagg
     2401 ggcgcccaaa ggtactccag ggcgaggctg ttcgttcgca cggactgatt ctgattcgga
     2461 tcaccgaaga tcgagggcgc gccgcgctgc tcgaatcgct ccttttcgtg ctccagcatc
     2521 agcgtgaccg cgtgacgcga gctcgcccgc agccgggtga gggaagtggc tttgaatcgg
     2581 gccgcggccg ttctgctttc ccgggcaccg tcggcgaagc tgcgattgtc ggtatcgaaa
     2641 taggagaggc gaatctgctg ctcgaaccgc tcgtcgccgt gccaggtcgc cgcgacgctg
     2701 ccgaggtact cgtcgtgctt cgagacgcga tccccgtcgc gcggctgaaa cgcaggaaac
     2761 gggatgggat cgaagtccgc gtcgctcttc aggtgatgaa cgagcgcctc gatggtccag
     2821 gattcgctgc ggtagccgcc gctcaagcgg tagctgctct ggtcgaagcc gtcgtcctcc
     2881 tcgccggtgg tggcgatgtt cgttccgtcg gttctgaatt cgctgagcgc gaggttgtag
     2941 tgatacgagc ccgtcgtgtc cgcgagctgc agcgccgcct ggcgcgtgtc gaacgacccg
     3001 ccttccaggg acaacctgcg ccggcgctcg gtgggcttcg tgatgaagtt cagcacgccg
     3061 gccaccgcgt cgctccccca gatggcgctg tgggcaccgg ccagcagctc gacgcgcgtc
     3121 gtgcccgtga gcgagaggtt ggcgaagttg aagctcgcat cggtggcagg gtctgccagc
     3181 tcgacgccgt ccatgaggac catgagatgg ttggcttcgg atcctcgaac gcgaacctgg
     3241 gtcacggagc ccaggctgcc ggcctggctg atggcgagac tcggcaggct gcgcaaggcg
     3301 tcggcgccca cggcgggccc ggtcccggtc gtgcgagtca ggacggggag caccggccgg
     3361 tccgtgcgta ttcgatgcgc ggttacggtg atggtctcca gctcggggtc gggctcggca
     3421 aaggagaggc tcgggacgag cagggccagc actgtggcca ggaccatggg catgacggca
     3481 gactccggcg gcctgcctca ccccgggcag ggttgctgat accgggatcg ggccggtctc
     3541 cggactcgcg agcatcaatg gcgtgcgccg ccttcccaac gtttcagcgt cagtggctga
     3601 gcctgctcag tggcggctcc ggcgcggccg tctcgattac cgttgcgggg gcagtgtcgg
     3661 atttccaccg acttcccgtt cacccgatcc caccgcgcgg caaccgcgca gcggcgccag
     3721 tatagctact cgaacccttt gatgcagtcg tatccgtaga gccagtccat gcgctgcatg
     3781 gcgatctccg gcaggaagcg gctggtcagc gacagtcgca gattgcggcg ccaggctttc
     3841 ccgccggggt ccgtggtcag ctctgccagg tgatgccgct gtccctggcg aaagcggccg
     3901 aggcgcacct tccggtagcg ctcgtagtcg gggaacccct tggccggctc gtcctcccag
     3961 cgctcgagca tgcgcgacag cacccaggcg tcttccatgg caagcgacgc gccctgctgc
     4021 aggtgcgggg tgacggggtg gcaggcgtcg cccaggagcg cgacccgccg gtcgtaccac
     4081 tgggcgatgg gttcccgttc caccggggca gaccgggtga cgccggttgc cgagtccacg
     4141 agacggaaga cgtcctcatg ccagttccgg aaggcgccgg ccagcttcgc ccctgctccc
     4201 gggtttcggg caaaggcaag gaaacgcgcg gcgcagccgt cggcagacgg ccactgggtg
     4261 aggtaggcgc ggggtccgag ccaggtggtg accgcattgg cgcgcagacc gattggcagg
     4321 tcggtgcccg ggcaggtgcc cgtccagacc acgtcctggg tcgtctgcgt cgtctcggcg
     4381 tatcccatgc ccgcgcgaac ggctgaggcc tcgccatcgc agccgatgac gacgtcgtga
     4441 cagatctcct gggagccgaa cgtcagcgtt acgcttcgcg catcctgagc gatgcgggca
     4501 cagggttggc ccgtgcgcac ggcgacgccg tgatgcgcgc atcgcgtcgc cagtgcttcc
     4561 agcagcgcct ggcgggcgac gacgacctcc ggcgctccgt accgggcctc catgaacgcg
     4621 ccgaggggtc ggtgcgccag cacgaagccg ctgcgccagc ttctctgcac gtaggcatcc
     4681 ggcttgtggg cacgagatag aaacgggccc aggtgcagcg cgcgcagcac gcgggtgccg
     4741 ttgggcgaca ggaccaggtc ggatggctca ggggagcccg gctcgccccg gtcgtagacg
     4801 gtgacgttgc gaaatccagc ttcgccgagg gcggctgcaa gggtcaggcc gccaatgccg
     4861 gcacccacga cccccaccga tggcgattca gccgtcccgc agcttccagc gtgtgccgtc
     4921 gcgggtgtcc tccagttcga tgccgagatc cgtcagctcc tcgcgaatct cgtccgcgcg
     4981 ggcgaagtct ttcgcctttc gggccgcgtt gcgttcctcg atcaagcgat cgatctcggc
     5041 ggcgtcaatg tcccgcccgg ccgtgaagtg cgctttcgcg gactcgatga gaatgcccag
     5101 cagccagccg ccggccagca gccgtcgtgc cagctcgacc ttctcggaag cgtccgacgt
     5161 gcgatgaatg gcgcttgcca gctgatgcat ggcggccagg gcctggggcg tgttcaggtc
     5221 gtcggcaagc gctgccacca cgacggtcgg gtactcttcg gcgggcaggt cgcggtagtc
     5281 gctggatgtc tcgtcctcca cgccggcatc acgcagcgcc tgatacagac gctccaggct
     5341 gctacccgcc tggtcgagga gatcctccga ccaggccagc tgggaacggt actgacccga
     5401 aagcagcgcg tagcgcagga cttcccctgg aaagcgatcc agaaggtcgc ggatggtgac
     5461 aatgttcccc acggacttgg acatcttctc ctggcccatg gtgagcatgc cgttgtgcag
     5521 ccagtagcgg acgtactcgg gatggtcgtt cgcgcagcgg ctctgagcag cttcgttctc
     5581 gtggtgagga aacgccagat ccgagccgcc gccgtggatg tcgatggtct ggccgagatg
     5641 cttgctgatc atcgccgagc actcgatgtg ccagccgggc cgcccccgtc cccaggggct
     5701 gtcccagccc ggaagatccg tcgtggaagg cttccacagc acgaaatcct tggggtcctg
     5761 cttgtacggc gctacctcta cccgcgcgcc gtcgagcatg tcctcgaggc tgcggtggga
     5821 aaggctgccg tattcgggat cggagggaac gtggaagaga acgtgcccct gagcctcgta
     5881 ggcgttgccc ttggcgatta gggtctcgat catcgcgatg atctctgcga tgtggtgtgt
     5941 tgccctcggt tccaccgtgg gctcgagcac gcccagcgcg gccacgtctt cccggtaggc
     6001 gctgctgaaa cggtcggcca gggcctggat gggctcgtcg ttgtcccggg cggcggcgtt
     6061 gatcttgtcg tcgatgtccg tgatgttgcg cgcgtagacc acctcggggt gcgcggcgcg
     6121 gagcagtcgg tagagcacgt cgaacaccac ggccgggcgc ccgttgccga tgtgcaccag
     6181 gccgtagacg gtaggcccgc agacgtacat cgttatgcgc tggggatcga cgggatcgaa
     6241 ggcttccttg cggccggatt tcgtgttgtg gagtcggatg tccataagcg gctcgccgtc
     6301 tgctcagacc ggttcggacg acacgcgcgc ggtgccgtcg ctgacgctaa ggtaaaagca
     6361 cgagggccgg ttcgtgtggc aggccggccc cgtctggtcc acgatgagca ggatggcgtc
     6421 gccatcgcag tcgaagcgca tctccttgag cgcctgcaga tgtccggaac tctcgccctt
     6481 gcgccagaag gccccgcggc tgcgagacca gtagcaggcg tagccgtctt tcagggtgcg
     6541 ttcgaccgcc tcgcggttca tccaggcgag catgagaacg tccctgctgt cggcgtcctg
     6601 ggcgatgacg ggcaccaggc cgtccgagtc gaacttgacc gcgtccagca gcgcgtcgag
     6661 ggcgacctcg tggccgagct ccgcgtcctc gaggtattcg aacatctcag cctccgaatc
     6721 gttcctgcag gtagccgaaa acggccctga tcgccatggc ttccgcaccc tcgggtcggc
     6781 ccgggcgggg gcgtgtgttg aaggccatga tgtcgaagtg cacccacggc gccgagccca
     6841 cgaacttctc caggaagagg gcggccgtta tggatccggc gaacggcgag cttgccgagt
     6901 tgacggtgtc ggccaccttg ctgtcgagca ggtaggcgta gggtttgtgc agcggcagcc
     6961 gccacacagg gtcgtcccag cggtcgccgt gggcgctcag cccggcggcg acctcgtcat
     7021 cgttgcagaa catcgccgcg atctctgcgc ccacggccga tcgcgcggat ccggtcagcg
     7081 tggcgaaatc gaccatgagg gcgggttcgt cctcggcacc cagcgccagg cagtcacaca
     7141 tgaccatgcg cccttcggcg tcggtattgt cgatttccac cgagatgccc ttgtaggtcg
     7201 gcacgacgtc gccaggccgg taagcgttcg cgccgacggc gttctccact gccgcaatga
     7261 gcacgcgcag acgaacgggc agattgctcg ccatgatgag ctgggcgagg ccgagagcgt
     7321 tggctgcccc gcccatgtcc tttttcatga gccgcatact ggcggccggc ttgatgtcga
     7381 gcccgccgct gtcgaagcac acccccttgc ccaccagcac gatcagcgga tggtccgggt
     7441 ctccccaggt gatgtcgatc aggcgcggcg cgtcggccgc cgcccgccca acggcgtgaa
     7501 tcgcgtcgaa gccgccctcc agcagctgct cgcccaccag cacccggcag tttgcctggt
     7561 tggcgtccgc cacctggatg gcctcgtctg cgagatggct cggcagcatg tcgcctgcgg
     7621 ggatgtttat gagatcgcgc accagggtag tggccgcgtc catgttcgca acgaggtccg
     7681 cgtcgttgcg tgcaggaatc aggagctggg cgggagctct gtcgcgctcc cggtagcgga
     7741 cgaactgata ggaaccgagc gcccagccga gcagctgcag gtcggagacc tcggttttca
     7801 gctcgtacac gccttcgggt atgcgggtgg gaaggccgcc caggctggcc aggtcgtccc
     7861 tgccgtccca tcctgccacc agctcggccg ggtggccgtc ggctccgggc agccaggcga
     7921 actcgccgct cttcgccgcg aactgagcac gccggatcca gctccgttcg gcttccgaaa
     7981 gctcgctggc aaacgcctcg aagtccgagg ttctgatcag cctgagcggc gttgcggcgg
     8041 cgtcggatga aacgatgagc gcttctgcca ttggggctcc ggttggttga agtccgagat
     8101 ggtctatgat ggcctcccgg ttcgcaagcg gtctgagccg tgctcacgga agtcaccacg
     8161 catcacctcg agctcaccga tccggatcag ttcaagccgg cggccaccgg tccggcctac
     8221 gcgctcaagc aggtccagcg tcccaccgcg gagctgaacc gctttctcta ctcgacggtc
     8281 ggggcggcgt ggttctggta cgagcgcttg agctggtccc gggcccgctg gctcgagttt
     8341 ctcgaccggc cggaggtcgt cacttgggtc gcctatgtgg atggcagccc cgccggctat
     8401 ttcgagctgg aggatcagcc gggcgggtcg gtggagatct gctatttcgg cctcctgccg
     8461 gcctacatcg gtcagggcat gggcggcgct ctgctgacgg atgccatccg cgccgcctgg
     8521 gccaggggcg cgtcccgcgt ctggcttcac acctgctcgc tggatcaccc ttttgcgctc
     8581 gccaactatc tggcgcgggg cttttccctg ttcgacatcg tcacgaccac gaaaaacctg
     8641 cccgatccca tgccggagcc ccggcccggg gatgccgagg cgggccggtc gtcagattga
     8701 tttgaccggg atccgcccga gcccgaggtt gttgccttcg agtatgcggt cggcccggta
     8761 gctcgacctt accagcgggc ccgacgcgac ttccgtgaag ccacgctcca agccccattc
     8821 ccgataccgt tcgaactcgt ccgggtgcac ccagcgcgcc actgccagat ggttgcgggt
     8881 gggacgcagg tactggccca gggtgaggat gtcgacgccg tgctcgcgca gatcgtccat
     8941 ggcctggcaa atctcgtcat cctgctctcc caggccgagc atgaggctcg atttggtgag
     9001 caagtcccga cggacctgct tggcctcggc gagaacgccc agcgtctgat cgtatcccgc
     9061 ccggggatcg cgaacgtcgc cggtcaggcg ctccacggtt tccagattct gggcgaagac
     9121 gtccagcccc gagcccacca cgacggcaac ggcctcggcg gatccgctga aatccggcgt
     9181 gagcgcctcg acggcggtct cggggttgac cgccttgatg gcctggatgc acttcgcgta
     9241 gtggccggcg ccgccgtccg ccaggtcgtc ccggtcgacg gaggtcagca cgacgtagcg
     9301 cagccccatc agacgcacgg actccgctgc gtttgcgggc tcgtccggat cgagacgccc
     9361 tccgggattg cccgtgtcaa ccgcgcagaa ccggcaggcc cgggtgcaca cggagcccat
     9421 cagcatgatg gtcgccgtgc cgttgttcca gcactcaccg atgttcgggc agtgagattc
     9481 ctcgcacacg gtggccagac ggtgggtggc caccgtcctg tgcactgcct ggtacttttc
     9541 gccgccgccg agccggacgc gcagccacgg gggcttgcgg ccggtagccg gctctgcctg
     9601 cccgttcgcc ttcatgccgt ccttgatggc ggtgaaacct tcgggcgtgc ggaatttctt
     9661 gccgctgacg actggtttgc tcatggcccg tcattgtacc cataatgccg cgtcgttgag
     9721 gtgcggtaac caatgcagga acgtccggca gtcacccggc gggtgcgatg gctatacggg
     9781 gccggagccg ccgcctacgg cgtgaaggaa aacggcttct cctatttcct gctcttctac
     9841 tacaaccagg tgctcgatct tccgggcgcc tatgccggca cggcgatcct gatcgccatg
     9901 atcttcgacg ccatctcgga ccctctggtg ggcgtctggt cggacaatac caggtcgcgc
     9961 tggggacgcc gccacccctt catgtacagc gccgccctgc ccgtggcggt gatctacttc
    10021 tttctctgga acccgccgga gctaacggag tttcagctct ttgtctatct caccgtaggg
    10081 tgcgtgctcg tgcgtttctt tgtcacgctg tacgagatac cgtccagcgc catcgtcgca
    10141 gagctcacgg acgactacga cgaacggacc cggttgctca gctaccgcta catgatgggc
    10201 tggttcggcg gtctcgggat ggcggtgctg aactggggtt tgttcatggt ggtctgggga
    10261 gaggcgggcg aaacgacgtt ccgcgtctac ggagcggtgg gtgcggtgat catgttcgtg
    10321 gccatcgtgg gatcctccct cggcctgcac cgctacatac cctacctgca cccgccgccg
    10381 gaaaggacct cctaccgggt ccggcagctt accggcgatc tcatcgagac catcagcaac
    10441 aggaacttcg ccgcgctgtt cttcgccggc ctcttcgccg cggtgggcgc cggcgtggcg
    10501 acgaatttcg acacctacat catcacccac ttctgggagt tcgaggccga gcagtggcgc
    10561 tgggtgatca tctcgttgtt cgtctcggca ctcattccca tgtatctggc gccgctggtt
    10621 acccggcgct gggacaagaa gcgaagcgcg ctgggtatct acgctttcca gatcgtcttt
    10681 gccgccctgc ctatcctgtt gcgcctggcc ggctggtttc cggataacga cagcccctgg
    10741 acctacccgc tgatctgggc tcacgccgtc gtcaacgtgg tcgtgatcgt gatgttcggc
    10801 gtcatccaga gctcgatgct ggcggacgtg gtggagcaca gccaggcgtc caccggccgg
    10861 cgtgaggagg ggctgttctt cgccgcgcga tcgtttgccc agaaggcgac ctcgggggtc
    10921 ggcgcgttca tcgccgggct ggcgctggac tggatcgcgt tcccgagggg cgcgacgccg
    10981 gggacggtgc cggacgccgt catatgggat cttggtttca tttacggccc cgcgctcatg
    11041 attttctacg tgctggcgtt ggtttccatc gggttctata ggatctcgcg gcagggacac
    11101 gatcagcggg tgggcgcgct gcgcgcgggc gccacggccc cgtcgggaag catggaggaa
    11161 tcgcgatgaa cgaccgaccc gtgatcgtca ccgggggcgc cgggggcatc ggcagcgcca
    11221 tcgttgcccg tcttgttgcc gacggccatc cggtggccgt gctcgatctg caccccgcgt
    11281 ctgctacccc ttcgggctcc ctgttttcct acccgtgcga catcgccgat ctcgacgcgg
    11341 tggagcgggc ggtcgccgag ttcgaggcgg cggtgggcgc gccgttcggg ctcgtcaaca
    11401 atgccggctg ggaccaggcc ctgccgtttc tggagacggg gccggatctg tgggagaagg
    11461 tgatcgacgt gaacctgcgc ggccccgtga acgtcaccta cgcggtgctg cgcaggctgg
    11521 cgggcgcggg ccgcggccgg gtggtcagca tcgcctcgga tgccggccgt gtgggctcgt
    11581 cgggagaggc cgtctactca gcgtgcaaag gcggcatcat cgccttcatg aagacgctgg
    11641 ctcgtgagca cgcgcgtcag ggcatcacgt tcaactccgt ctgccctggc cccacggaca
    11701 cgcctctgct cgattccttc gacgagtcgg gtcggctggc ccaggcgctc gagcgggcca
    11761 tacccatgcg gcggctggga agaccggagg actttccggg gatcgttgcc ttcctgttga
    11821 gtgacgaggc gtcgttcatc accggccaga ccgtgagcgt ctctggcggg ttgtcgatgc
    11881 aggggtgacc tacccctcgt cctgaaactg cagcgagtgc agggtgtgat agagcccctc
    11941 gctggcgatc agctcctcat gggtgccgag ctcgaccacc cttcctccct gaagaaccag
    12001 cacccggtcg gcttcctgaa tggtctgcag gcgatgggcg atgatgatcg aagtccgccc
    12061 ggcggtgagc tttcgcagcg cgtcctggat gaggagctcg gtttcggtgt cgatgctcgc
    12121 ggttgcctcg tcgagcacga ggatctcggg atccgcggcc agcacccggg cgaaggccag
    12181 cagctgccgc tgcccctggg acaggttctg accgcgctcg gagagctcgg tgtcgtatcc
    12241 gcggggcagg tcgctgatga agccgtgggc gttgacgcgg cgcgcggcct cctcggcgcg
    12301 ctcccgggtg atccggggat cgccgagggc gatgttgtcg aagacggtcc ctgagaagat
    12361 gtggaagtcc tggagcacca cgccgatgcg ctggcgcagg tcgcgcgtat ggatgcggtt
    12421 gagatcgatg ccgtcgagga aaatctggcc gtcgtcgaat tcgtagaacc ttccgagcag
    12481 gcggatcagc gtgctcttgc cggaccccgt cgggcccacg atggcgagct tctccccggc
    12541 cccgatctcg aaggagacgt cgtggagaat ctgaacgtcc gggtcgtagg cgaagttcag
    12601 gtgcctgaag gtgacctcgc cctgcagccg gggcggcagc gtgacgggat cggccggctc
    12661 gtgaatcttc tcgtcccagt ccagcgcctg aaagatccgc tcgccgctcg ccatggcccg
    12721 gaagagaatg ttcgtctgct cgccgagaac cacgatggga tggaagagca tgtcgatgaa
    12781 gcgggtgaac agaatcacgg cgcccagggt catttcttcc tgcactgcag cgccgccggc
    12841 gtaccagatg atcaccccga gggccaggtg cgccatgctg tcgttgaagg cgccgtagag
    12901 ggtctcgaat cgcgtcgttc tgagctcgtg gccgcggttt tcttcgttga tcttcgtgta
    12961 acggtccagg ttgtattgct gtcgctcgga gagctgcacc acctgaaggc ccgccaggtt
    13021 ttcctgcatg ttctgattca gagccgacag cgacatgcgc accatccgga acaggcggcg
    13081 cgtcgcccgg cggaaataga aggtcacgct gcccatgacg ggcaacgcga gaagcagaag
    13141 cagggttagc tcgacgtcga tggccagcat gatggcgagc gccacgaaga agggcatgaa
    13201 ctcgccgatg agcgagccga gaccgcgaag cagctcgtag agcgcttcga tgtcgttggt
    13261 cacccgcgtc atgacccgtc ctacggcgac tcggtcgtag aacgaggacg gtctggtctc
    13321 gaggtgtcga aacagatcca gccgcaggtc cctcagaccg ttgatgaccg acgagatcag
    13381 ggtcatgcgg tgagcgtgac cggcagccgc ccaggcaatc tgcagccccg catacaggag
    13441 acaggccgcc atgagagggt gaagtgccag gatgtccgcc agccactcgg tcagctccac
    13501 catgccgagg tccggtgcca gggaatccgt ctcgccccgg atcaggtggt cgatcaccac
    13561 cagcgagatg atgatgggca tgagaacctg cagcgtggac gccagcagca ccagcacggc
    13621 gctgattccg aaggtcaccc ggtagggccg caaccatttc aggaggcgtc gcaggagcgc
    13681 aaggttcaga accgcgccgg agatctcggc gtcgaacatg gagtagttgc gccgctcggg
    13741 agcgccaggc tggctggccg ccgtcacgtc gtcgccccga tcaggtcgta gtcggtatca
    13801 acagcgccct cgcgctgaat ccgttcgagc tcggcgtact gtcctgccct ggccagaagc
    13861 tcttcgtggg taccggactc gacgatgcgg ccggcgtcca tgaccacgat gcggtcggag
    13921 tggcgggcgg tcgaaacgcg gtgggacacc aaaatggtgg tctggcggcg ccgcagccgg
    13981 cgcagctccc ggagaatgtg ctcttccgtc tcggtgtcga cgctggaaaa gcagtcgtcg
    14041 aggatcagca cgggcgcccc gcgaatgagg ccgcgggcca gggtggctcg ctgcttctgg
    14101 cctccggaga gcgtcacgcc gcgctcgccg acgatggtgt cgagctgctc cgggaactcc
    14161 tcgatggttt ccttgagatc tgcggacgtt gccgcctccc agatcttctc caccgcccgg
    14221 cccgggtcgt cgtaggtcag gttgtcgccc aggggctcgc cgaacagaaa agcatcctgg
    14281 ggcaccatgg caacctggga cctcagctgg gcgagtgggt agtcgcggac gtcgtggccg
    14341 tcgatgagaa ccgcgcctgg cggcgtgtcc agcagccgca cgagctgctt gagcagcgtc
    14401 gtcttgcccg atccgaccct gcccatgatg gtcacggact cgccccgggc gacggtcagc
    14461 gagacgtcat cgagagccgg acgaatgccc tcgtcgtaag cgaaggtcag gtggcgtatc
    14521 tcgacgtcgc cccggatcgt cgccggcgtc gtcccgcttg gatcgtcggc gatctctggg
    14581 gacagggtga tgacctcgaa cagccggtcg ctggccacgg ccgcccgctg gaagaggttg
    14641 acgatgaagc cggccacccg aaacggctgg acgaccatgt tcacgtacat gaagaaggca
    14701 acgagctcgc ccaccgtcat ggcgccgtcc aggaccaggt agccgccata ccccaggatg
    14761 gtgacggagc agatggcggc cagcgaaggc atccaggccg ccatgagcga gttgatctga
    14821 gactggcggt agaacgcgtt ggcgtactgc tggttggtcc tgccgaaccg gcggatctca
    14881 ttctcttcct ggaccatggc ctggatggtg cgaatcccgg agaggttttc ctgggcgtgg
    14941 gcgcccaggt cggacaatcg gttctgaacg tccctcgagg ccacgcccat ctggcgcgcg
    15001 gaccagcttg cgtagaagaa caccaggggc aggggtggca gcagcagcag cgtgagtgac
    15061 ggggacagat agagcatgaa gccgaagccc accagcgtcg cgtagacgag gatgaccatc
    15121 aggatcgtgc ccatggcgat gagtctctgg atcaggccga tgtcggctac cgcccgggtc
    15181 atcatgtcgc cgatggtgta gcgcgtgaag aaacgaacgc cctgttgctg cagggattcg
    15241 tacagagcct gccgcagatc gaacgccacg tgctggccga cgcagcgcac ggagtagcgg
    15301 gcgtaggtga ccacgacgta gcgcccgatc accgctccgg caattccgag cagtggggtc
    15361 gtcatgtccg cgttgccggc ggacatccga tcgatgccga tggccacgaa gataggcacc
    15421 atggcttcga agaatcttgc gattccgaac gcgccgacgc cggcccccag tcgcagccga
    15481 tacggggcga ggaacgattt cagacggatc agagagcgaa tcatcggtgg ggcgcagccg
    15541 ctgccgcgtc agaaactggc gttgtgcggc actctgggga acgggatggc gtcacggatg
    15601 ttgtccatcc ccgtcatgta aagcaccagg cgttccagac cgagcccgaa ccccgcgtgg
    15661 ggtgcggtgc cgtaccggcg aaggtcccgg taccaccaga gatcctcgcc gatgtcctgt
    15721 tccgccatgc gggcgtcgag cacgtccagg cgctcttccc gctggctgcc gccgatgatc
    15781 tcgccgacgc cggggacgag cacgtccatg gcggccaccg tcgcgtcgtc gtcgttcagg
    15841 cgcatgtaga acgctttgat ggtcttgggg tagttgacca ccaccaccgg cccgcccacg
    15901 tgcacctcgg tgagatagcg ctcgtgctcg gattgcaggt cgagtcccca ctgcaccggg
    15961 tactcgaagt tctgacccgc cttctcgagg atgctgacgg cgtccgtgta gtcgatccgt
    16021 tcgaacggtg tggcgatgac gtgttccagg cggctgatgg cctgctcgtc tacccggtcg
    16081 gcgaagaaac tcatgtcgtc gggacaggcg tccagcaccc tgcggcagac ggctttcagg
    16141 aagtcctcgg caagctccgc gttggcgttc aggtcggcga aggcgatttc cggttcgatc
    16201 atccagaact cggccagatg tcggctggta ttcgagttct ccgcgcggaa ggtcgggccg
    16261 aacgtgtaca ccttgctcag agcaaggcag tagctttcca ggttcagttg ccccgagacg
    16321 gtcagaaagg tctcgcgacc gaagaagtcg tcgtcgaagg ggctgtcccc gcgggtcagt
    16381 gcccggttgg cgatgtccag cgtggagacg cgaaacagct gcccggcgcc ctcgcaatcg
    16441 ctggccgtga tgatcggcgt gttgacccag acgaaaccgt tgtcgttgaa atagccgtgc
    16501 accgcctggg cgatggcgtt acgcacgcga gcgatggcgc cgaaggtgtt cgtgcgggga
    16561 cgcagatggg cgacttctct gaggtattcg aaggtgtggc gtttttttgc gatcgggtaa
    16621 gtttcagggt cgtccaccca tcccaccacc acgacctcgg tggcctgaat ctcgcggtcc
    16681 tggccgcggc cttgagattc caccacggtt cccctgatcc gcacggagca gcctgtgccg
    16741 agatgctcga catcgtgctc gtagttggcg agctgcgagt cggccaccgc ctgaatggtg
    16801 tcgaagcagg agccgtcgct gacgtgcacg aaggagaagc cgcctttcga cgttcgcctc
    16861 gatctgaccc aaccttcgat ggtgacctct gctccgacgg gaaggcggtg ggcgaggatg
    16921 tctttgatgg tgtcggtcat cggcgcggct gctcaggcag gaatgaggga ttgtggaatg
    16981 ataatggaat tggcccggcc ttggataatt ccccgcgggt cgtggtcata atgagacatg
    17041 cgcaacctca tcatcgtcat cgccctcgtg ctggttctgc tcggcggcct ctggctggct
    17101 tcggatcgca gtgccggtga tcgggaagtc ccgctcccgg gcgccgagcc ggtgccggtt
    17161 cccggaccgc ggcccgctga acccgcgcgg cgaccgcagc cgatgcccgt cgctgagccg
    17221 gctatcgtgc tgcccgacct cgacgcgagc gatccgttcc tgcgggaacg gctggctggg
    17281 gtcgagttgc cccagaactg gatcgatcgt gatgatctcg tccgccgcct ggcggtggtg
    17341 atcgaaaacg cggctcgcgg agagtatcca aggcgtcagc tgggttttct tgcgccgtcc
    17401 ggccgtttcc gcgtggtgga aagacagggc aggctcttcg tggaccccga caactaccga
    17461 cgcttcgacg ggtatctcga cgtgctcgaa ggcctggacc cccggacggc cgccgagctg
    17521 ctgtctctca tcgagcctct gctggccgtc gccatgggcg agctcggcag cgacgggtcg
    17581 gtttccgatc agatcgatgc tgccgtcgcg gaggtgatgg cggtgcccga cgttgtcggc
    17641 gaggtggagc tggctcagcc gaaggtgctc tacgagtacg cggatccggc gctggaagcg
    17701 ctgtcgccgc tgcagaagca ggtgcttcga atgggtccgg aaaatgcggc gcggctcaag
    17761 cgctacgttg ccgagatggc cgtcgcgttg cagcctgacc cctccgacgg ctaggcgggc
    17821 gcccttcggc gcaagccgtt cagccgatgt ccagggtgcg ggtattgtgg aacttgtcca
    17881 ggctgacggt ggacttgaag gggctgcgtt cctcgatgta ctcgatgtcc tcgcggacgt
    17941 gctcgcgctc ctcgtccaga tagcgggcaa cggcattacg aaagcccgcg tcggctatcc
    18001 agtgggcgct gtaggtaggg cagggtagat acccccgggc gaccttgtgc gggccctggg
    18061 cgccggcttc cacccttttc agtccccgct cgatagcgaa gtcgatggcc tgatagtagc
    18121 aggcttcgaa gtgcagaaag gggtggtgct cgatgcaacc ccagttgcga ccgaagaggg
    18181 cttccccgcc gatgaagttg agcgcgcccg cgatgtagcg cccgtcccgg ctgcacatga
    18241 tcagcagcac gtcctcggcc atggtctcgg tgacgaggct gaaaaactgc cgagtgaggt
    18301 atggggaccc ccattttcgg gatcccgtgt ccatgtagaa gcggtagaag gcgtcccagt
    18361 gcgattcctg gatgtcgctc cccgtgagcc attcgatcct gatgccggtg gccagagcgt
    18421 cccgccgctc gcggcggagg ttcttgcgct tcttcgagga gagatcgccg agaaactcct
    18481 cgaagctccc gtagcctgcg ttgtgccagt ggaactgctg atccatgcgc cgaagaaagc
    18541 cgagctcgcc ggcgatctgc cactgggcct ccggcaggaa ggtgacgtgc agggacgaga
    18601 ctttgatctc tgccgctacc tgggcacacg cccccagcag cgcccgctcg atctccggcc
    18661 gcgagacctc atcttggccg gcggcaagca cgcgccgacc ggttgccggc gtgaacggaa
    18721 cgctgacctg cagcttgggg tagtagtcgc cgccggcccg atgccaggcg tcagcccagg
    18781 cgtagtcgaa cacgtactcg ccctgggagt ggttcttcac gtacatgggc accacgccca
    18841 gaatacggtc ggcttcttca aggaccaggt gaaagggttg ccagcccgtc ctggccgcgg
    18901 cgctgccgct gtgctcgagg gcagccagaa actcgtggcg caggaacggg tcgtggggaa
    18961 tctcgccgcc tggcatcgcg caccggttcc agtcttcggc cggtatctcg tcgatgctgt
    19021 gcagaattct cgcggtgcgg gaagtcaatc ggttctcctt cgttccgagt ttaaccgctc
    19081 gccgcaaggt gcgctgccct ggcaggcgaa cgcttcagac aataccggcc tcgaggccgg
    19141 cggccagggt caggccgagc tcttcgcagc ggtcgatgtg gtcgttggtg atctctcccc
    19201 gggcgatgac gggctcctgg acttccttga acggatatcc gttggcgatg cgtcggatgg
    19261 cgcgcagcgc cccgctgccg tcgtttccgg cgctgatgaa gatcgcgtag ggcagggaaa
    19321 gaatcttccc ctccacgggg tagaacgtcc ggtcgagaaa gtccttcatg ccgccggaca
    19381 tgtagccgaa gttctcgggc gtgccgagca ggaggccgtc ggcccagagg agatcgtctg
    19441 cccccgcgtt ctgcgccgtc agcaggcggg cgtccacgtc gctcacgagc tcgttggtgg
    19501 cacctctgtg gacggccgcc gccagctggc cggtgcggcc ggtctgcgtg tggtagacga
    19561 tgagaagatg tttcatctgc tggcgggagg tgggcgaggc tggtgggtcg tcgagtatac
    19621 tgacgccttt tcagaagttc gatcgtgacc cagctcgacg acattactca ggcccggcag
    19681 cgcatctgcg gccagattga gctgacggcc tgcagccgct cagcgacgct gtcggcaatg
    19741 acgggcgcgg acgtccatct caagttcgag aacctgcagt tcacggcgtc gttcaaggag
    19801 cgcggagcgc tcaaccggtt gctccagctg acgggcgagc agcagcgctt cggcgtggtg
    19861 gccatgtcgg cgggcaatca cgcccaggcc ctggcctatc acggcaagcg gctgggcgta
    19921 cccacctgca tagtcatgcc ccggttcact ccgaacgcga aggtcgagca gacgcgcgtc
    19981 cacggcgcgg aagtgctgct gcacggcgcc gagttcgacg agacgctggc gttcaccgaa
    20041 gccctggccg aggagcgcgg cctcacactc attcacccct tcgacgatcc ggcggtcatt
    20101 gctggccagg gcaccctggg gctggagctc gtggagcagg tcccgggact cgaggcggtg
    20161 ctggttccgg tggggggcgg gggcctgatc gccggggtgg cgacggcggt gaaatcggtg
    20221 aagccggagg tggagttgct cggcgtgcag gtggaacgct tcgcttc
//
LOCUS       DBNHOX010000317         2106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1439162, whole genome shotgun sequence.
ACCESSION   DBNHOX010000317 DBNHOX010000000
VERSION     DBNHOX010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1439162"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /gene="spt"
                     /locus_tag="AC16UZ_06960"
     CDS             <1..1121
                     /gene="spt"
                     /locus_tag="AC16UZ_06960"
                     /EC_number="2.3.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004758 - serine C-palmitoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046513 - ceramide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine palmitoyltransferase"
                     /protein_id="HIR6246461.1"
                     /translation="PTDVIIERVISATESVIGGKPTIMAGTNNYLGLTFDPECIAAGK
                     RALDESGTGTTGSRMANGNYASHGELEREIADFYGYPYAMVFSTGYAANIGTMAALVG
                     PGDAVMLDGDAHASLFDGSKLSGATVYRFKHNDADSLDKRLRRLGDVASRTLVIVEGL
                     YSILGDRAPLKELAEVTRAHDACLFVDEAHSLGVLGETGRGRVEEDQVEADFVVGTFS
                     KSLGSTGGFCVSPHRALTLFRYASRPYMFTASPCPSVIATTRAALAQMRKRPELRASL
                     WRNAETLYSGLAQLGFTLGPEPSPVVAVRMTSREQALANWQQLLDNGIYINLILPPAA
                     PDGTALLRCSVSAAHTPEQIQRIIDAFRQLPPDPGQPA"
     gene            complement(1084..>2106)
                     /locus_tag="AC16UZ_06965"
     CDS             complement(1084..>2106)
                     /locus_tag="AC16UZ_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR6246462.1"
                     /translation="LDVATASLAALCMKIVMLLAGPALGLPEELAMPAVLYGLVLLAS
                     GSGTARGVLRVAGRFHAMGVQQNVGPLIRLAGVAAVYLAEGGLLGYLMVWAVAFAAEH
                     LFMNVRGWLELKRRGVRPGRPTLAGAKRRFPGLWGFVGTLYWQSNLDLAQRHGLILLA
                     GVLLGPAGAGMFRVAREFADVLAKPVIVVRQAVFPDFARLWRERDPRFKTLYWRLGLY
                     GGLIAAVVVGLVGLYAGELLEALVGEEYVHGAALLVWLMVAGALELIGAALRPAAYAM
                     GAATGALRVQIVTAVCHVSAFAVLVPWLGLAGAGIAAACSGAIALAGMVALVLTRADQ
                     DPGAAA"
BASE COUNT          409 a          761 c          654 g          282 t
ORIGIN      
        1 acccgaccga cgtgatcatc gagagggtca tctcggcaac ggaatccgtc atcggcggca
       61 agcccacgat catggcgggg acgaacaact acctcggcct gactttcgac ccggaatgca
      121 tcgccgccgg caagcgggcg ctggatgaat cgggcaccgg caccacggga tcccgcatgg
      181 ccaacggcaa ttacgcttcc catggggagc tggagcggga gatcgccgac ttctacggct
      241 acccctacgc catggtgttc tcgaccggct acgcggcaaa catcgggacc atggcagctc
      301 tggtgggtcc gggagacgcc gtgatgctcg atggcgacgc ccacgccagc ctgttcgacg
      361 gcagcaaact ttccggcgca accgtttacc ggttcaagca caacgatgcc gatagcctgg
      421 acaaacgcct gcgccggcta ggtgacgtcg ccagccgcac gctggtcatc gtcgaggggc
      481 tctacagcat tctgggagac cgcgctccgc tcaaagagct ggcggaggtg acccgcgccc
      541 acgatgcctg cctgttcgtc gacgaggctc actcgcttgg cgtgctcgga gaaaccggac
      601 gcggacgggt ggaggaagac caggtggaag ccgacttcgt cgtcggcacc ttcagcaaga
      661 gcctgggttc cacgggcggc ttctgcgtga gcccccaccg agcgctgacc ctgtttcgct
      721 atgccagccg cccctacatg ttcacggcgt ccccctgccc gtcagtcatc gccaccacgc
      781 gggcggccct tgctcagatg cgcaaacggc ccgagctgcg cgcgtcgttg tggaggaacg
      841 ctgaaacgct ctacagcggc ctggcccagc tgggattcac cctcggcccc gagccgagcc
      901 ccgtggtcgc cgttcgcatg acctcccggg aacaggcact ggcgaactgg cagcagctgc
      961 tggacaacgg tatctacata aacctcatcc tgcccccggc cgcgccggac ggcaccgcgc
     1021 tgcttcgctg cagcgtcagt gcagcccaca cgccggaaca gattcaacgc atcatcgacg
     1081 cgttcaggca gctgcccccg gatcctggtc agcccgcgta agcaccagcg cgaccatccc
     1141 tgccagcgca atcgctcccg agcacgcagc ggcgatgccg gcacccgcca gccccagcca
     1201 gggcacgagg acggcaaaag cagaaacgtg gcatacggcg gtgacgatct ggacccgcag
     1261 cgcgccggtc gccgcgccca tggcgtaagc cgccggcctg agagccgcac cgatcagctc
     1321 caacgcaccc gcgaccatca gccacacgag cagcgccgcg ccgtgcacgt attcctcgcc
     1381 cacgagcgct tcgagaagct cgccggcata taggcccacc aggccgacga ccacggcggc
     1441 gatcagcccg ccgtaaaggc cgaggcgcca gtagagcgtc ttgaaccgcg ggtcgcgctc
     1501 ccgccacaga cgggcgaagt ccgggaacac ggcctgacgc accacgatca cgggcttggc
     1561 cagcacatcg gcaaactcgc gggcgacgcg aaacatcccg gcacccgcgg ggccaagcag
     1621 aacgcctgcc agcaggatca ggccgtgccg ctgcgcaaga tccaggttcg actgccagta
     1681 gagcgtgccg acgaagcccc acaaccctgg aaatcgtcgt ttcgcgccgg caagggtggg
     1741 acgtccgggc cggactcctc gccttttgag ctccagccag ccacgcacgt tcatgaagag
     1801 atgctcggcg gcaaacgcga cggcccaaac catcaggtaa ccaagcaagc ctccctcagc
     1861 caggtacacg gcggctacgc cggccagccg gatgagcggc cccacgttct gctggacgcc
     1921 catggcatga aatcgccctg ctacccggag cacgcctcgc gcggtccccg agcccgatgc
     1981 cagcagaacc aggccgtaga gaacggccgg cattgccagc tcctccggga gccccagggc
     2041 ggggcccgcg agcagcatga cgatcttcat gcacagcgcg gcaaggctgg cagtggcgac
     2101 atccag
//
LOCUS       DBNHOX010000318         2797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1182692, whole genome shotgun sequence.
ACCESSION   DBNHOX010000318 DBNHOX010000000
VERSION     DBNHOX010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1182692"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="AC16UZ_06970"
     CDS             complement(<1..145)
                     /locus_tag="AC16UZ_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HIR6246463.1"
                     /translation="MVEARLPCPVCLGSMMEKEAPDGRRDLVLDLCPRCGGIWFDLGE
                     VQLL"
     gene            complement(138..581)
                     /locus_tag="AC16UZ_06975"
     CDS             complement(138..581)
                     /locus_tag="AC16UZ_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246464.1"
                     /translation="MGKTIGGVLLCLIALFMGVGFLQVGALSWASLLALVIGAGIPGG
                     LGLTLLVRQFREPRRVAAREALRRQTIDSELVRLAGELGGKLTVVEVVARLALPAEEA
                     GAALDELARSGVCEIQVSDSGVLVYDFADLRHLGEKEQARGLLDG"
     gene            839..1555
                     /locus_tag="AC16UZ_06980"
     CDS             839..1555
                     /locus_tag="AC16UZ_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6246465.1"
                     /translation="MTITLDRPEVKNALTHEMYAAVADALAKAEGSDEVRCVVITGSG
                     DVFTAGNDLGDFAKPLPEGTWPAGRFLEVLRDMDTPVVAAVNGAAIGVGLTMLLHCDL
                     AFAAESATFSAPFTRVGVVPEAGSSLLLPRALGNAWANDILLAGRVLTAADALVAGLV
                     SRVVPADQLQDVARGVARQVATLAPNSTRESKRLIRADRDQIAERMERETKAFRAQLS
                     SAEVAESLLAIREKRQPIFD"
     gene            complement(1613..2260)
                     /locus_tag="AC16UZ_06985"
     CDS             complement(1613..2260)
                     /locus_tag="AC16UZ_06985"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6246466.1"
                     /translation="MPADTPCSPEHLLASFGQIDIYLFDQLLRGRITPRMRVLDAGCG
                     SGRNAHYLLRCGADVFGIDADRAQVDEMRATAAQHAPGLCQDNFLVARLDDIPFPDAF
                     FDSVICCAVLHFAADEADFEAMMKEMWRVLRPGGVFFARLASSIGIEDRVDRLRGRWH
                     HLPDGSQRFLVDERYLTALTSKLGASLLDPLKTTNVQNMRAMTTWVLAKPVDGAS"
BASE COUNT          501 a          869 c          973 g          454 t
ORIGIN      
        1 gcagcagctg gacctctccg agatcgaacc agattccacc acagcgcggg cagaggtcca
       61 gaaccagatc gcgtcggccg tccggagcct ccttctccat catgctcccg agacagactg
      121 ggcacggcag ccgggcttca accatcgagt agcccgcgtg cctgctcctt ctcgccgaga
      181 tgccgcaggt ccgcgaagtc gtacacgaga actccagagt ccgagacctg gatctcgcag
      241 actcccgagc gagccagctc gtcgagtgcg gccccggctt cctcggcagg aagtgcgagc
      301 cgagcgacca cttcgacgac ggtcagcttg ccaccgagct caccggcgag ccttaccagc
      361 tccgagtcga tcgtctgacg ccggagcgct tcgcgggcgg cgacccgccg cggttcgcgg
      421 aactgtcgca caaggagcgt caggcccagg ccgccgggga tgccggctcc aatgacgagg
      481 gcgagaagtg acgcccacga aagggctccg acctgcagga atccaacacc catgaagagg
      541 gcaatcaggc acaggagtac cccgccgatc gtctttccca accgttacct cttgggttcc
      601 gcagcaatgt ccatgaccga accaattgat tcgagacgcg gaggcaggcg caaggccagg
      661 tgtcagtgac atccaccgcg agcgtccttg cgacccgacc ttggtgcccc cttcgcggcg
      721 gccgctgtag gttggcaggt gctggcgccg gactcggaga gcgccgcccg agctcgctcc
      781 ctctttcacg gatcggctga tgtcgcaatt cgttcgcgtt gaagtgcacg agaggttgat
      841 gacgatcacg ctcgatcggc ccgaggtcaa aaacgcgctg acccatgaga tgtatgcggc
      901 ggtggcggac gctctggcca aggccgaagg cagtgacgag gtgcgctgcg tggtcatcac
      961 gggatctggg gatgtcttca cggctgggaa cgacctgggt gacttcgcga agccgctgcc
     1021 cgaggggacc tggcccgcgg gtcgcttcct cgaggtgctc cgcgacatgg acacaccggt
     1081 ggtcgccgcc gtcaacggag cagcgatcgg cgttggcctg accatgctgc tgcactgcga
     1141 cctggctttc gcggccgaga gcgcgacgtt cagcgctccg tttacccgag taggtgtggt
     1201 gcccgaggca ggatcctcac tgctgctgcc cagagcgttg ggcaatgcct gggccaacga
     1261 catccttctc gccgggaggg tgctgaccgc cgcggacgcg ctggtggcgg gcctcgtctc
     1321 tcgcgtcgtt cctgcggacc agctgcagga cgtggcacgg ggcgtcgcga gacaggtcgc
     1381 tactctggcg cccaactcga cgcgcgagtc gaagcgattg atcagggcgg accgggatca
     1441 gatcgctgaa cggatggaac gagaaacgaa ggcgttccga gcgcagttga gctccgcgga
     1501 ggtcgccgag tcgctcctgg caattcgaga gaagcgtcag ccgatcttcg actgagcggt
     1561 gacgcctcgg tgcccgccac agccgggggc gagccgccgc gacccgaggt ccctagctag
     1621 cgccgtcgac cggtttggcg aggacccagg tcgtcatggc ccgcatgttt tggacgttcg
     1681 tggtcttgag agggtcgagg agcgaagcac cgagcttcga ggtgagggcc gtcaggtagc
     1741 gctcgtccac cagaaagcgc tgtgagccgt cgggcagatg gtgccagcga ccgcgcagcc
     1801 ggtcgacgcg gtcttcgatg ccgatgctcg acgcgagtcg cgcgaagaag acgcccccag
     1861 gtcgaagcac cctccacatc tccttcatca ttgcctcgaa atccgcctcg tcggcggcga
     1921 agtgaagaac cgcgcaacag atgacggagt cgaagaaggc gtcggggaag gggatgtcgt
     1981 cgagccgggc caccaggaag ttgtcttggc acagccccgg agcatgctga gcggcggtgg
     2041 ctcgcatctc gtcgacctgc gcacgatcgg cgtcgatgcc gaagacgtcg gctccgcagc
     2101 gtaggaggta gtgtgcgttg cggccgctac cgcaccctgc gtcgaggacg cgcatccggg
     2161 gtgtgatgcg accgcgcagc agctggtcga agagatagat gtcgatctgg ccgaaggacg
     2221 ccagcaagtg ctcgggagag cagggtgtgt cagctggcac gtcggctcac tggggctgct
     2281 tgctcctggg cacagatccg acgacccgag gtcatgtcac gtgacggatc aattgattca
     2341 cgacgtgcag gcgggcgcaa gacggatggc tggagtcgct ggcgcgtgag ctgcgcctcc
     2401 ctgcgctggt agagactacc gcggacctga gagtctgacg gagcgcccgc gcagcggccg
     2461 agccgtaggg gtgtcccgag ctgctgcgcg ggccaaggcc ccgaatcgct gaaggcgggc
     2521 aggggcgacg gcttacgggc cgaccagtgt accggctttg ggtcgtgccg cagtgacccg
     2581 aagttcccgc ctgatcccct gtacgtcgtg tgtgagctga ggcgagagcg tgagtcaggt
     2641 gttgtgcgac atgatgtggt gaccgagggt ccgacgaatc cgagcgtcaa cgggatccga
     2701 tacgaaactc ctcgttgggc gggggaatca cagctgtctc gagaggacgc gatagctggg
     2761 gcacccactc gtagggtagg cgatgtgccc ggaagat
//
LOCUS       DBNHOX010000319         2294 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_154717, whole genome shotgun sequence.
ACCESSION   DBNHOX010000319 DBNHOX010000000
VERSION     DBNHOX010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_154717"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="AC16UZ_06990"
     CDS             complement(<1..649)
                     /locus_tag="AC16UZ_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HIR6246467.1"
                     /translation="MISDVTWMPGQKPFWWACCLIVCWLIQPAVADDDGRGRPYFKGS
                     PANPTALMRRMEADAHSAQALSAASSVGCVDGFADGFPCHNVHLQALVPLSTAGLSSA
                     SANDVWGWTDSISGREFALIGLNDGTGFVEITDPANPVVLGKLPTRNWNSTWRDIKTY
                     SDHAFIVSEADGHGMQVFDLTRLLDASPDITEAAHYDAFGSAHNIVINEDSGYAFA"
     gene            complement(810..1167)
                     /gene="ssrA"
                     /locus_tag="AC16UZ_06995"
     tmRNA           complement(810..1167)
                     /gene="ssrA"
                     /locus_tag="AC16UZ_06995"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1288..>2294
                     /locus_tag="AC16UZ_07000"
     CDS             1288..>2294
                     /locus_tag="AC16UZ_07000"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6246468.1"
                     /translation="MLAAALAAALAATLAATPAAAQAPAQEPPLDSRSFIERSVTLLK
                     EEEYALARTYLAPALIDYRLTPGERSRAYYLRGFTFFAEGFYVSAGKDYARALEFNPD
                     NPVALAAMADLYFRGLGVEADYVIAYQLYSKAAADGHANAQFHTGYLELEGIGIEKNL
                     DSARGWLHKAADQDVVPAMIHMARSYRTEATDSPDPDQARAWYERAFAAGSADALVAL
                     AFMHQDGDFGAPDPEKALELFTRAAQEGSGAAQVSLAHMHITGELLPQDYAKALELLQ
                     QAARQGVPASYLGLGHIYDTGLGVKPDRATAENWYRLAAEAELLPAQLRLVRLLLRED
                     D"
BASE COUNT          438 a          750 c          693 g          413 t
ORIGIN      
        1 cggcaaacgc gtagccgctg tcctcgttga tgacgatgtt gtgggcgctg ccgaaggcgt
       61 cgtaatgggc cgcctcggtg atgtcgggcg aggcgtcgag cagcctcgtc agatcgaaca
      121 cctgcatgcc atgcccgtcc gcctcgctta cgatgaacgc gtgatccgag taggtcttga
      181 tatcgcgcca ggtcgaattc cagttcctgg tgggaagctt tcccagaacg accggattgg
      241 cggggtcggt aatctccacg aatccggtgc cgtcgtttag accaatcagg gcgaactcgc
      301 gtcccgagat cgagtcggtc cagccccaga cgtcgtttgc gctggcgctg gagagcccgg
      361 ccgtactcag cggaacaagc gcctgtaggt ggacgttgtg gcagggaaag ccgtcggcaa
      421 acccgtccac gcacccaacg ctgctcgccg ccgacagcgc ctgggcgctg tgcgcatcgg
      481 cctccatgcg cctcatcagc gccgtcgggt tggccggaga acccttgaag tacgggcgtc
      541 cgcgaccgtc gtcgtcggca acggcgggct ggatcagcca gcaaacgatg aggcaacagg
      601 cccaccagaa cggcttctgc cccggcatcc aggtgacatc gctgatcatg cttctcatct
      661 ccaccccgac cttacagcga tcactcagat atataagaaa ctaaatttac aacatgttcc
      721 gatcagccac aatccgttcg cgagaggcac aaaaaagccc gcactgggcg ggctttgcgg
      781 acgatcctgg acttgcccgg atcgcgtatt ggtggaggcg gcgggagttg aacccgcgtc
      841 cgttggcact cggctgttgg ctctacatgc ttagcttccg tctattgatt tagcctcgag
      901 cgacccgacg gtcagggtgc tgtcagcgag cccgattggt tttaacgctt cagccccggg
      961 cgagacgtcc acgcgatcct gcaaactttg ccgtcgactt cgccttgcag gccaagctcg
     1021 tcgacgtaag cgggtttttt aggccgctag tgcgtagttt tcgtcgttgg caactatatt
     1081 aagttacagc gattgtttaa cgagagtcac taccctctcg gcatgcaccg caagctccgt
     1141 tacccccgtc gaagccaaat cgcccccatt tacgaggtga cgcgaattgt atcacgcgtc
     1201 aaatataacg ttactgcata ataactgact gatttcacga gaattggttc ccctatcaac
     1261 atgccccgtt ttggtgtttt gaaacctgtg ctggccgccg cactcgccgc cgcactcgcc
     1321 gccacactcg ccgccacacc ggccgcagcg caggcgccag cccaggagcc gccgctggat
     1381 tcccgcagct tcatagagcg gtcggtaacg ctgctgaaag aagaagagta cgcgctggcc
     1441 agaacctatc tcgcgccggc actcatcgac tatcgcctga caccgggcga gcgctcccgc
     1501 gcctactacc tgcgcggctt caccttcttc gccgagggct tctacgtctc cgcaggcaaa
     1561 gactacgcgc gggccctgga gttcaacccg gacaaccccg tggccctggc cgccatggcc
     1621 gacctctact tccggggcct gggcgtggag gcggattacg tcatcgccta ccagctctac
     1681 tccaaagcgg ccgccgacgg ccacgcgaac gcccagttcc acaccggtta cctggaactc
     1741 gaaggtatcg gcatcgagaa gaacctggac agtgccaggg gatggctgca caaagccgca
     1801 gatcaggacg tggtgcccgc catgattcac atggcacgca gctaccggac agaggccacc
     1861 gacagcccgg atccggacca ggcccgagcc tggtacgaga gggctttcgc cgcgggaagc
     1921 gcagacgcgc tggtggctct ggccttcatg caccaggacg gggacttcgg cgcgccggac
     1981 cctgaaaagg cgctggagct cttcacccgg gctgcccagg aaggctccgg ggccgcccag
     2041 gtcagcctgg cgcacatgca catcaccggc gagctgctgc cacaggacta cgccaaggcc
     2101 ctggagctgc ttcagcaggc ggcaaggcag ggggtgccgg ccagctacct ggggctcggg
     2161 cacatttacg acacgggcct gggcgtgaag cccgatcgcg cgacggccga gaactggtac
     2221 cgactggcgg ctgaggccga gctgctcccg gcgcagctcc gcctggtcag gctcctgctc
     2281 cgggaggacg acag
//
LOCUS       DBNHOX010000320         1501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1311415, whole genome shotgun sequence.
ACCESSION   DBNHOX010000320 DBNHOX010000000
VERSION     DBNHOX010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1311415"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1501
                     /locus_tag="AC16UZ_07005"
     CDS             <1..>1501
                     /locus_tag="AC16UZ_07005"
                     /inference="COORDINATES: protein motif:HMM:NF019410.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HIR6246469.1"
                     /translation="FELIEEDLREKTLASLGDGLGEIYDSGRELIEKYRNQIRNDFIG
                     GVLRQISNVAQLEDIAPLRSRREEAIRREHEQAGSDKFDGWLDVAEVVAKAESRYRDD
                     LRDLRARLGLVEPPWRHKEVVPVGPAAITWAFDGALKDLELTPRIRTHVFEGFQAALL
                     PALNDFYHGLGELLSECEIFPTEEALEAISALQEPARDDDLTLHEYVPGSARHGEASG
                     DEPDAEEEAAVGVKLATHDEPEAEQVGPAPREVYATARSIVKLERVARQQLEGEGGEP
                     RQAAGGGAANPFGSEEILRVLGALGPGGQGDRATVCQRLVQELGAADGDRSLGESDLD
                     RFRVLESLIDSICEDRFLSQGSLNWVRRLEVTLYKLAVHNPGFPGGDPDRPHPAEIML
                     NQLARLSGIADSREAKARELSGRVDALVERVADGYSGDGGIFEEAVAELNPLVDEQIR
                     NYRGNMERIVRASEGQQRLTRARKAVVNALAPKMAGKEVPEPILKLLNPG"
BASE COUNT          276 a          459 c          535 g          231 t
ORIGIN      
        1 ttcgagctga tcgaggaaga tctgcgcgag aagacgctgg cgagcctcgg cgacggcctg
       61 ggcgagatct acgattcggg ccgggaattg atcgagaagt accgcaacca gatccgcaac
      121 gacttcattg gcggcgtgct tcggcagata tccaacgtcg cgcagctcga ggacattgcc
      181 cccctgcgct cgcgcaggga ggaggcgatc cggcgtgagc acgagcaggc cggaagtgac
      241 aagttcgacg gttggctgga cgtcgccgag gtcgttgcca aagccgaaag ccgctaccgg
      301 gacgacctgc gcgatcttcg cgcacgtctc gggctcgtcg agccgccctg gcggcacaag
      361 gaagtggtgc ccgtgggacc tgctgccatt acctgggcgt tcgacggcgc gctgaaggat
      421 ctggagctga ccccgcggat ccgaacccac gtcttcgaag gctttcaggc ggcgctgctt
      481 ccggcgctca acgacttcta tcacggcctt ggcgagctgc tttccgaatg cgagatcttt
      541 cctacggaag aagccctcga agcgatcagc gctctccagg agccggcccg cgacgacgac
      601 ctgacgcttc acgaatacgt accggggtcc gcccggcacg gcgaggcttc cggcgacgag
      661 ccggacgccg aggaggaagc cgcggtgggc gtgaagctgg ctacgcacga tgagccggaa
      721 gcggagcagg ttggccccgc gccgcgcgag gtctacgcca ctgcccgcag cattgtgaaa
      781 ctcgagcgcg tcgccaggca gcagcttgaa ggcgaggggg gcgaaccgcg gcaggcagcc
      841 gggggcggcg cagccaaccc gttcggcagc gaggagatcc tccgggtgct gggcgcgctg
      901 gggccgggag gtcaaggcga ccgggccacc gtttgtcagc gcctggtgca ggagctgggt
      961 gcggccgacg gcgacagaag cctcggcgag agtgacctgg accgttttcg ggtgctcgaa
     1021 agcctcatcg actccatctg tgaggaccgg ttcttgagcc aaggcagctt gaactgggtc
     1081 cggcgcctgg aagtgactct ctacaagctg gcggtacaca accctggttt ccctggaggg
     1141 gatcccgacc ggccccaccc ggccgagatc atgctcaatc agcttgcccg tctcagcggc
     1201 atcgcggatt cccgggaagc gaaggcgcgt gagctgagcg gtcgcgtgga tgcgctcgtg
     1261 gagcgtgtcg cagacggcta ctccggggac ggcggcatct ttgaagaggc ggtcgccgag
     1321 ctgaatccgc tggtggatga gcagatccgg aactaccgtg ggaacatgga gcgcatcgtg
     1381 cgcgccagcg agggacagca acgcctgacc cgggctcgaa aagcggtcgt gaatgccttg
     1441 gctccgaaga tggcgggcaa agaggtcccg gagccgatac tcaagctgct gaatcccggg
     1501 t
//
LOCUS       DBNHOX010000321         2989 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_604764, whole genome shotgun sequence.
ACCESSION   DBNHOX010000321 DBNHOX010000000
VERSION     DBNHOX010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_604764"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..465
                     /locus_tag="AC16UZ_07010"
     CDS             262..465
                     /locus_tag="AC16UZ_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246470.1"
                     /translation="MNEIHLLLGLVLSAIGIGYLVYGRRQQAPVFFIAGLGLVSIPFF
                     VGSTSTLMVAGAVVGGAPFLIRL"
     gene            473..796
                     /locus_tag="AC16UZ_07015"
     CDS             473..796
                     /locus_tag="AC16UZ_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246471.1"
                     /translation="MRAVAIIIAAALLAVSPVVTGDRSAAGIRVSESQRYCQAARQCT
                     KVQTRCGGCDCGTAINESFAAEHRDRLAALCGSHEQVECERSCPETRPICVIGVCALE
                     PLRGS"
     gene            801..1247
                     /locus_tag="AC16UZ_07020"
     CDS             801..1247
                     /locus_tag="AC16UZ_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246472.1"
                     /translation="MSFLPAGFHVPELLVTPTFSARPLTVDDVVRDYDAVMSSRERLV
                     GTFGSESSWPAADLSLKQNLIDLGWHQKEFQKRRSFAYTLVDPDDTLTIGCFYVYPEA
                     PDGFDASVYLWVRTSHSDMDEAVFSAVKQWLEEAWPFDRLACPGRN"
     gene            complement(1270..1731)
                     /locus_tag="AC16UZ_07025"
     CDS             complement(1270..1731)
                     /locus_tag="AC16UZ_07025"
                     /inference="COORDINATES: protein motif:HMM:NF021525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HIR6246473.1"
                     /translation="MLRFGGVCWLLAIALLLGACDPQDRRPGLWLTGEVADVAPGDWA
                     FSDEYAEIALEVATPYLIPHSVTIWCAQVDGTLYVAAAAPEEKNWPGWVSDDPDVRLK
                     IGDTVYAATLAELEDETEIAAVRSAYSGKYEVANLGSGASLRYWRVGPRGA"
     gene            1969..2229
                     /locus_tag="AC16UZ_07030"
     CDS             1969..2229
                     /locus_tag="AC16UZ_07030"
                     /inference="COORDINATES: protein motif:HMM:NF025715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HIR6246474.1"
                     /translation="MSDEDIRRALQALHTELENADPIDPELVSLLKEVDSDIHRVLEA
                     STPALEGLGDRLEWLAAKLAVSHPRVEGFLRELVDALGKLGI"
     gene            2239..2610
                     /locus_tag="AC16UZ_07035"
     CDS             2239..2610
                     /locus_tag="AC16UZ_07035"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6246475.1"
                     /translation="MIDGGCFCGEVRYRIEDGDYPAAHCHCTMCRRTSGAPFVSWLVV
                     PKEKFSFTAGTPKQLQSSDRGRRFFCSSCGTPMTCIVDDHPDNVDVTLGSLDEPERFV
                     PRFEIHTDTRLGWVQTDLKQA"
     gene            2628..>2989
                     /locus_tag="AC16UZ_07040"
     CDS             2628..>2989
                     /locus_tag="AC16UZ_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HIR6246476.1"
                     /translation="MYALLADLLVVLHLLFILFVVLGGFLTIHWPRLAWAHLPAVAWA
                     VVVEWAGWLCPLTPWEARLRSLAGEAGYQGDFVAHYLLPLLYPQAMTRTTQVLLGALA
                     LAVNAAIYGGWWYLRRRR"
BASE COUNT          511 a          976 c          946 g          556 t
ORIGIN      
        1 ggcccggtta tcacatgaac aaggccctct ggaacacggt cacgctcgac ggatccattc
       61 ctgcgggcga gctggagcgc atgatggacc gatcatacgc tctggtcgtc cgaggcctca
      121 gaaaagcaga gagaacgggg ctggagatac gccacggcaa agaggccgtc taccgttagc
      181 ttcaggcatc gatcccgggg cgtctgcaaa ggtgttcagc acgctgttat agtcctcccg
      241 ccagcaatcg ctgaggagcg gatgaacgaa attcaccttc tcttgggcct ggtgctcagc
      301 gccatcggca taggctatct ggtatacgga cgccgccagc aggcgcccgt ttttttcatt
      361 gccggtctgg ggctcgtttc gatcccattt ttcgtcggct ccacgtcaac gcttatggtc
      421 gccggggccg tcgtcggtgg ggcgcctttc ctgatcaggc tgtgagcccg ccatgagggc
      481 cgttgcaatc atcatcgccg ctgcgctgct cgcggtctca cccgtggtga ccggtgaccg
      541 atcggctgcc ggcattcgcg tcagcgaatc ccaacgctac tgccaggcgg cacgccaatg
      601 caccaaggtc cagacccgct gcggcggctg cgattgcggc accgccatca acgagtcgtt
      661 tgccgccgag caccgcgatc ggctggccgc gctgtgcggc agtcatgagc aggtagaatg
      721 cgagcggtcg tgccctgaaa cccggccgat ctgtgttatc ggggtgtgcg ccctggagcc
      781 gctgagggga tcatagtctc gtgagctttc tgccggcggg ttttcacgtt ccggaacttc
      841 tggtcacgcc caccttcagc gccagaccgc tgaccgtgga cgacgtggtt cgcgactacg
      901 acgcggtgat gagcagccgt gaacggctgg tcggcacgtt cggctcggaa tcgagttggc
      961 ccgccgccga tctttccctc aagcagaatc tcatcgacct cggctggcac cagaaggagt
     1021 tccagaagcg ccgctcgttc gcctacacgc tcgtcgaccc ggacgacacc ctgaccatcg
     1081 gctgcttcta cgtttatccc gaggcgccgg acggattcga tgcctccgtc tacctgtggg
     1141 tgcgcaccag ccactcagac atggacgagg cggttttctc tgctgtgaag cagtggctgg
     1201 aggaggcctg gccctttgat cggctggcct gtccggggcg caactgaccc gctcgagcag
     1261 ttgccccggt cacgcgcctc gcgggccaac ccgccaataa cgcagcgacg cgcccgagcc
     1321 caggttcgcc acttcgtact ttccggaata ggcgctgcga acggctgcga tctccgtctc
     1381 atcctcgagc tcggcaaggg tcgcggcgta gacggtgtcg ccgatcttca gcctgacgtc
     1441 agggtcatcg gatacccagc ccggccagtt cttctcctcg ggggccgctg cggcgacgta
     1501 cagggtaccg tccacctggg cacaccagat cgtcaccgaa tgtgggatca gatacggtgt
     1561 cgccacttcc agcgctatct cggcatactc atccgaaaat gcccagtcac cgggcgcaac
     1621 gtctgccacc tcgccggtga gccacagccc cggccggcgg tcctgagggt cgcacgcgcc
     1681 cagcagcaac gcgatggcaa gcagccaaca gactcctccg aagcgcaaca ttttcgctcc
     1741 ccaaacagca gacaacgcga cgatactagg agcgaaagcg cggcttgtca ccacgactcg
     1801 aaaagcgcga cgggccggcg gagcgcgttt gcatttcagg gcgatctatt ggcagattgg
     1861 ctcttgagat ggcaacgggc cgacgttgcc gcagcggcca gcccgcagga acggggtcag
     1921 cggccaaccc ggcaaccgcc gaacgaaccc aacgggagga gacagcaaat gagcgacgag
     1981 gatatccgca gagcgctgca ggcgcttcac accgagctcg agaacgcaga ccccatcgat
     2041 cccgagctcg tgtcgctgct caaggaagtg gactcggaca tccatcgggt gctggaggcg
     2101 tccacgcccg cgctagaggg gctgggcgac aggctggagt ggctggccgc gaagctggcg
     2161 gtctctcatc cccgggtgga ggggttcctc cgcgagctcg tggacgctct tggcaagctg
     2221 ggaatctagg aggcgaagat gatcgacggc ggatgtttct gcggcgaggt tcgctaccgc
     2281 atcgaggacg gtgattaccc cgcggctcac tgccactgca ccatgtgtcg tcgcaccagc
     2341 ggcgcgccgt tcgtcagctg gctcgtggtg cccaaggaaa agttctcatt cacggcgggc
     2401 acaccgaaac agctgcaatc ttccgatcgc gggcggcggt tcttctgctc gagctgcggc
     2461 acgcccatga cctgcatcgt cgacgaccac ccggacaacg tggacgtcac gctcggcagc
     2521 ctggacgagc cggagaggtt cgtacccagg ttcgagatac ataccgacac ccggctcggc
     2581 tgggttcaga ccgacctgaa gcaggcctga gcgctatctt gtctgcgctg tacgcgctgt
     2641 tggccgacct gttggtcgtg ctgcatctgc tgttcatcct gttcgtcgtt ctcggtggct
     2701 tcctgaccat ccactggcct cggctcgcgt gggcacacct gcccgcagtt gcatgggccg
     2761 ttgtggttga gtgggcgggc tggctgtgcc ccctgactcc gtgggaagcc cggctgcggt
     2821 ccctggccgg cgaagccggc taccaaggtg acttcgtcgc ccactacctc ctgccgcttc
     2881 tttatcctca ggcgatgacc cgcacgaccc aggttctgct gggcgccctt gcgctggccg
     2941 tgaacgccgc gatctacggg ggctggtggt atctccgccg gcggcgttg
//
LOCUS       DBNHOX010000322         2064 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_668319, whole genome shotgun sequence.
ACCESSION   DBNHOX010000322 DBNHOX010000000
VERSION     DBNHOX010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_668319"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="AC16UZ_07045"
     CDS             complement(<1..362)
                     /locus_tag="AC16UZ_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR6246477.1"
                     /translation="MSIIKASDQLIRSMTPPAQHSLAIETTAALGQKLEGKSLPRYIV
                     IEGPIGVGKTTLANKLAEAFHYPILLEPAAQNPFLNSFYRDGRRNALPTQLFFLLHRA
                     QQIANLDHADLLGPTLVA"
     gene            complement(377..1645)
                     /gene="pcnB"
                     /locus_tag="AC16UZ_07050"
     CDS             complement(377..1645)
                     /gene="pcnB"
                     /locus_tag="AC16UZ_07050"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0043633 - polyadenylation-dependent RNA
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HIR6246478.1"
                     /translation="MSEPVRLDATEHGLLRDQVDPEALDVTRRLSEAGYQALLVGGCV
                     RDLLLGAEPKDFDVATDATPEEVRQLFRRSRLVGRRFRIAHVRYGRKVIEVSTFRRSH
                     TEDEDERRHSEEGVILRDNLYGSLEEDAFRRDFTINALYYDPETEEILDYVGGLEDLE
                     RADLRFIGEAEVRLREDPVRLLRAVRFQAKLNFQLDESIETALPDAAERLSVIPSARL
                     FDEVAKLMMSGAAGAAWKAIAGTPLRAALFPGTPPDSRLVELAMANTDQRIASDLPVT
                     PGFLLAVLMWHDFQARCNALTDERKPAEARHQAASETLAAQQAVTSMPRRFSQFVRDV
                     WSLQPRLEARLPRQIDRIVQHPRFRAAYDFLVLRAESGEQATDSEPLDDAAQWWTTFQ
                     QADQATRADMTEARLAVQPPRKRRRRRRRG"
     gene            complement(1642..>2064)
                     /locus_tag="AC16UZ_07055"
     CDS             complement(1642..>2064)
                     /locus_tag="AC16UZ_07055"
                     /inference="COORDINATES: protein motif:HMM:NF052145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-type ATPase lid domain-containing protein"
                     /protein_id="HIR6246479.1"
                     /translation="LQLAESILRRTCKRLSRTCPGFTRSAQEAMQQYAWPGNVRELEN
                     AVERAVILCSESAIEPHLLAIDLTPPASVEPPPSMNDRSSLEDYFVRFVTAHQDQLTE
                     TELAEKLGISRKSLWERRQRLNIPRKKTRKRGPRRDST"
BASE COUNT          327 a          640 c          701 g          396 t
ORIGIN      
        1 tcggcgacca gggtgggccc gagcaggtca gcgtggtcga ggttggcaat ctgctgcgcc
       61 ctgtggagca ggaagaacag ctgggtgggc aacgcgttgc gccggccgtc tcggtagaag
      121 ctgttgagaa acgggttctg cgcggcgggc tccaggagaa tcgggtagtg aaaggcctcg
      181 gccagcttgt tggcaagggt ggtcttgccg accccgatgg ggccctcgat gacgatgtaa
      241 cgcggtagag attttccttc cagtttctgc ccgagcgcgg ccgtagtttc gatggctaga
      301 gagtgctggg ccggtggcgt catggagcgg atcagctggt cggatgcctt aataatggac
      361 aaggcatccc ttcgcttcaa ccacggcggc gccttctcct ccgctttctt ggtggctgga
      421 ccgccagtcg cgcctcggtc atatcggccc gcgtggcctg gtctgcctgc tggaaggtag
      481 tccaccactg cgcagcatca tcgagcggtt cgctgtcggt ggcctgctcg cccgactccg
      541 cccgcagcac gagaaagtcg taggcggccc ggaatctggg gtgctgcacg atccggtcga
      601 tctgcctcgg tagcctggcc tccagacgtg gctgcaggct ccagacgtcg cgtacgaact
      661 gagagaaccg gcgcggcatg gacgtaaccg cctgttgagc ggccagggtc tcggacgccg
      721 cctggtgccg ggcctcggcg ggctttcgct cgtcggtcag agcgttgcat cgggcctgga
      781 agtcgtgcca catgagcacc gcgagcagaa agcccggggt gacgggcaga tcgctggcga
      841 tacgctggtc ggtattggcc atggccagct cgaccagccg ggaatcggga ggtgtgccgg
      901 ggaaaagcgc ggcccgcagc ggcgtgcccg caatggcttt ccaggcggct cccgcagctc
      961 cggacatcat cagcttggcg acctcgtcga acagccgtgc ggagggtatg acgctgagcc
     1021 gttcggcggc atcgggcagg gcggtctcga tgctctcatc gagctggaaa ttgagtttcg
     1081 cttggaacct caccgcgcgc agcagccgca cggggtcctc gcgcagccgg acctcggcct
     1141 cgccgatgaa gcggaggtcc gcccgctcca gatcctccag accacccacg tagtccagaa
     1201 tttcctcggt ttcgggatcg tagtagagcg cgtttatcgt gaagtccctg cggaaggcgt
     1261 cctcttccag gctgccgtac aggttgtcgc ggagaatgac cccttcctcc gagtgacgac
     1321 gctcgtcctc gtcttcggtg tgagagcggc ggaaggtcga tacctcgatg accttgcggc
     1381 cgtagcgcac gtgagcaatg cgaaatctgc ggccgaccag ccgggagcgt cgaaagagct
     1441 ggcgaacctc ctccggcgtt gcatccgttg ccacgtcgaa atcctttggc tctgcgccca
     1501 ggagcaggtc tctgacgcac ccgcccacga gaagggcctg gtagccggcc tcggacagcc
     1561 gcctggtgac atccagagcc tcgggatcca cctggtctct aaggagaccg tgctccgtcg
     1621 cgtctagcct gaccggctcg ctcaagtgct gtccctgcgc ggaccccgct tgcgggtctt
     1681 cttacgagga atgttcagcc gctgacgcct ctcccacagg ctcttgcggc tgatccccag
     1741 cttttccgcg agctcggtct ccgttagctg gtcctggtgg gcggtcacga accgcacgaa
     1801 atagtcctcc aggctggatc ggtcgttcat ggagggcggc ggctcgacgg aagcgggcgg
     1861 agtcaggtcg atggccagca gatgcggttc gattgcggac tccgagcaca ggatgacggc
     1921 cctttccaca gcgttctcga gctctcggac gttgccgggc caggcgtact gctgcatggc
     1981 ttcctgggcc gatcgggtga atcccgggca ggtcctcgag agtcgtttgc acgttcgcct
     2041 gaggatgctc tctgcgagct gtag
//
LOCUS       DBNHOX010000323         2474 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_990583, whole genome shotgun sequence.
ACCESSION   DBNHOX010000323 DBNHOX010000000
VERSION     DBNHOX010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_990583"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="AC16UZ_07060"
     CDS             complement(<1..262)
                     /locus_tag="AC16UZ_07060"
                     /inference="COORDINATES: protein motif:HMM:NF028042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR6246480.1"
                     /translation="MNGFTLVELMVAVAIMAIIAAVALPFYNQYSQRTYRTEAQADLL
                     ACAQAIERMAAVSFSYENAADTDADGVGDGNAGPVATVLCDPL"
     gene            complement(440..886)
                     /locus_tag="AC16UZ_07065"
     CDS             complement(440..886)
                     /locus_tag="AC16UZ_07065"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR6246481.1"
                     /translation="MVSSSDRRIDDNCRVTLHFAVLLESGEEVDTTRRSRPATFVIGD
                     GSLLPGFERALMGMKAGDDAQIPLEPGEAFGERQEGNVRRLPAKAFSGIDLEPGLLVS
                     FAAPEGELPGLVKCVDGSTVQVDFNHPLAGRRIIFDVSILRVEDVT"
     gene            complement(892..1416)
                     /gene="lspA"
                     /locus_tag="AC16UZ_07070"
     CDS             complement(892..1416)
                     /gene="lspA"
                     /locus_tag="AC16UZ_07070"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR6246482.1"
                     /translation="MNEKMGRAGMLKWLGLSAIVVVLDQATKWWIVQEFRLGERMDWL
                     PFLSWVRWHNDGAAFSLLSGAGGRWFFVALAVAFAVFIIWELRRLPVGGRLMGWAYAL
                     ILGGALGNGADRLFSGYVVDFVLVHYGSWHFPAFNVADSALTVGAAIWIGSILLDYLA
                     DRRDGQDPDEGPTA"
     gene            complement(1409..2335)
                     /gene="ribF"
                     /locus_tag="AC16UZ_07075"
     CDS             complement(1409..2335)
                     /gene="ribF"
                     /locus_tag="AC16UZ_07075"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR6246483.1"
                     /translation="MEIIRGLHNVKPRHRGCVLTIGNFDGVHHGHQVLLAHLIAKSEE
                     LGVPSMLMTLEPAPREFFAGETVPARLTRFREKVTLLEPTGLHRILCLPFNERIASIP
                     AEWIVEELLHNQLGIRYIVVGDDFRFGKGAAGDYRMLQAAGERYGFEVSHIGTLTFDH
                     ERVSSTRIRECLSAGDFALAEKLLGHPYFIMGRVVYGRQLGRKLGIPTANIQLQRYRA
                     ALEGVFAVSVRGLEKEYQGVANVGIRPTVDGREPLLEVHIFDFDRDIYGTLLTVEFRH
                     KIRDEIAFDGLDALKAQIQEDQRTARAWFANE"
BASE COUNT          492 a          836 c          728 g          418 t
ORIGIN      
        1 acagcgggtc gcacaggacg gtcgcgacag ggcctgcgtt gccgtccccg acgccgtcag
       61 cgtccgtatc cgccgcgttc tcgtaggaga acgacaccgc ggccatccgt tcgatggcct
      121 gggcgcaggc gagcagatcc gcctgagctt cggtgcggta cgtgcgctgc gagtactggt
      181 tgtagaaagg cagggcaacg gccgcgatga tcgccatgat cgccaccgcg accatcagct
      241 ccaccagagt gaagccgttc atacgtgatc gatcgtctgg tgtttccacg ggaccggatg
      301 aacgcatttt cggatcctga tcgttcgggc ccatcaatca taggcgcccg cgacgtcgac
      361 aaactatcca acggcgctga gcggtaggat tggagcgatg atctgtagga tacgcgtccg
      421 gcccagaaac gacggcggat caggtcacat cttcgacccg gagaatggac acgtcgaaga
      481 tgatcctgcg gcctgccagc ggatgattga agtccacctg aacggtgctg ccgtcaacac
      541 atttgacgag ccccggcaat tctccctccg gagccgcgaa cgaaaccagc agcccaggct
      601 ccagatcgat tccggaaaac gccttcgccg gcagccgacg gacattgcct tcctggcgct
      661 ctccaaacgc ttcgccaggt tccaggggga tctgcgcatc gtcccctgcc ttcatgccca
      721 tgagcgcccg ctcgaatccg ggcagcaggc taccgtcgcc gatgacgaag gttgccggtc
      781 gggaccgacg ggtggtgtcc acttcctcgc cggattccag caacacggca aagtgcagcg
      841 ttacccgaca gttgtcgtcg atgcgccgat ccgacgagct gacaatgagg ctcacgcggt
      901 cggcccttca tcgggatcct gaccgtcacg ccggtctgcc aggtaatcca gaagtatcga
      961 cccaatccag atggcggcac ccaccgtgag ggcggagtcc gccacgttga acgcgggaaa
     1021 atgccatgag ccgtagtgaa ccagcacgaa gtcgacgacg tagccggaaa aaaggcgatc
     1081 ggcgccgttg ccgagcgcgc cgccgagaat cagcgcgtag gcccagccca tcaaccgccc
     1141 gccgaccgga aggcgcctga gctcccagat gatgaacacg gcgaacgcga cggcgagggc
     1201 aacgaagaac cagcgtccgc cggcgccgct gagcaacgag aaagccgccc cgtcgttgtg
     1261 ccatcgcacc cagctcaaga acggaagcca gtccatgcgc tcacccagac gaaactcctg
     1321 gacgatccac cacttggtcg cctgatccag caccaccacg atcgcgctga gccccagcca
     1381 cttcagcatc ccggcacgtc ccatcttttc attcattggc aaaccacgcc cttgccgtac
     1441 gctggtcctc ttgaatctgc gccttgagag catcaaggcc gtcaaaggca atctcgtccc
     1501 tgatcttgtg acggaactcg acggtgagca gcgttccgta tatgtccctg tcaaagtcga
     1561 atatgtgcac ctccagcagc ggctcgcgcc cgtccacggt cggacgaatg ccaacgtttg
     1621 cgacgccctg atactccttc tccaggcctc gcacgctcac cgcgaaaacg ccctccaggg
     1681 cggcccgata gcgctgcagc tgaatgttcg cggtcggaat ccccagcttg cgcccgagct
     1741 gccgcccgta taccacccgt cccatgatga agtaagggtg gcccagcagc ttttccgcca
     1801 gcgcgaagtc tcccgccgac agacactccc tgatccgcgt gctgctgact cgttcatgat
     1861 cgaacgtcaa cgtgccgatg tgactgacct cgaacccgta acgctccccg gctgcctgaa
     1921 gcatccgata gtcgcccgcg gcgcccttcc cgaacctgaa gtcgtcaccc accacgatgt
     1981 agcggatgcc cagctggttg tgcagcagct cctccacgat ccattcggcc gggatgctgg
     2041 cgatgcgctc gttgaaggga aggcatagga tcctgtggag ccctgtgggt tccagcagcg
     2101 tgaccttctc gcgaaaccgg gtcagccggg cgggaaccgt ttccccggcg aaaaactctc
     2161 tgggcgcggg ctcgagcgtc atgagcatgc tcggcacgcc cagctcctcg cttttcgcga
     2221 tgagatgggc gagcaggacc tggtggccgt ggtgcacacc gtcgaagttg ccgatcgtga
     2281 gcacgcagcc acgatggcgg ggtttgacgt tgtggaggcc tctgatgatt tccatggcgc
     2341 accattatag gcgcctcacg cccgatggag caggttccgc ggccgctcgc cgaaaacgat
     2401 gaggcaaagc aggtacacgc tgccaccgcc cgccaccacg cccgccagcc agacgacgcg
     2461 ctcgccatcg ggcg
//
LOCUS       DBNHOX010000324         3002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1182944, whole genome shotgun sequence.
ACCESSION   DBNHOX010000324 DBNHOX010000000
VERSION     DBNHOX010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1182944"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="AC16UZ_07080"
     CDS             <1..471
                     /locus_tag="AC16UZ_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6246484.1"
                     /translation="IKERLAVPVADRLHQWISDLRELSGRIPRRYATLEEAYERMQTE
                     NPHLSEEQARHLTIHGSNQNEDGTYSWKFDNYVRAFPPIGIDFEEQYKLHGRITCPTL
                     LIRGTESWASDPSEDGRAARFQNATVRNIERAGHWVHHDQLDEFLEIVDGFLAD"
     gene            474..1535
                     /locus_tag="AC16UZ_07085"
     CDS             474..1535
                     /locus_tag="AC16UZ_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246485.1"
                     /translation="MSQRAVILHRSPWQGVFHVTGGGSGLLAELLTTPGASGTVLEAV
                     VPYAQAALADLLGRAPDQACSPSTARSLAMSAFDRARDLGAREPFGLGCTASLATERM
                     KRGKHRACIAVQTTTSTYSATLQLEGDRASEERDLVELIWHALSRTLELHLESAPPDT
                     FDGGLTSALEHWRALVLGEETAHATAPHDGRLLLPGSFNPLHRAHRRMLELAEHLTGL
                     EGAYELSIVNVDKPLLDYTEIEERLSQFEAPVWLTRLPTFVDKARYFPGAHFAVGVDT
                     IIRITEAGYYGSQADRDAALDELATLGTRFLVFGRILNDAFLTLSDLDLPATFRALCR
                     EVTEAEFNEPISSTEIRRR"
     gene            complement(1550..2440)
                     /locus_tag="AC16UZ_07090"
     CDS             complement(1550..2440)
                     /locus_tag="AC16UZ_07090"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF018533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fpg/Nei family DNA glycosylase"
                     /protein_id="HIR6246486.1"
                     /translation="MPELPDLTVYVEHLERRLVGQPLEAIRIANPFVLRSVEPTVDEV
                     CGRRILGCHRLAKQLVMELEGEHFVVVHLMIAGRLKWQKPSAPLPKPNALAAFDVPAG
                     SLLLTEASSKKRASLRLVRGAHALGALNPGGLEVLSADLAAFASRLRESNHTLKRALT
                     DQHVFAGIGNAYSDEILLRARLSPFKRPRDMADGEIQRLHEATKGVLGEWTDRLRAAA
                     GDDFPSKVTAFHPEMSVHGKYRQPCPVCGAPVQRIVYAENEANYCARCQTGGRLLADR
                     SLSRLLKENWPKRVEELEGG"
     gene            complement(2433..>3002)
                     /locus_tag="AC16UZ_07095"
     CDS             complement(2433..>3002)
                     /locus_tag="AC16UZ_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR6246487.1"
                     /translation="GTEAFAAYNIGANMLMVCMTVGFGFSIAGATLVGQHLGANDPDG
                     AARSGWRSTGLAVLAMGGLGGVVCFYAEELAVFFIGDEPLTVDYTVQFTYILGAMMPL
                     LAVEFTIGGALRGAGDTRFPLMATFLGLIGMRCGLAALATYLGVPVFWVYAALIGDYL
                     LKGVLLISRFRGGRWKTAIRYREPEALGA"
BASE COUNT          532 a         1042 c          978 g          450 t
ORIGIN      
        1 atcaaggaac ggctcgccgt accagtggcg gaccggctgc accagtggat ctccgatctg
       61 cgcgagcttt cgggaaggat cccccgtcgc tacgcgaccc tggaggaagc ctacgagcgc
      121 atgcagacgg aaaatcctca cttgagcgag gagcaggcac ggcacctgac gatacatggc
      181 agcaaccaga acgaggacgg cacctacagc tggaagttcg acaactacgt acgggctttc
      241 ccgcccatcg gcatcgactt cgaggagcag tacaagctcc atggccgcat cacctgcccc
      301 accctgctga ttcgcggcac cgagtcctgg gcgtccgacc ccagcgagga cggccgcgcc
      361 gcgcgcttcc agaacgccac cgtgcgcaac atcgaaaggg ccggacactg ggtccaccac
      421 gatcagctcg acgagtttct ggaaatcgtg gacggttttc tcgccgactg agaatgagtc
      481 agcgcgcggt cattctgcac cgctccccct ggcagggcgt gttccacgta accggcggcg
      541 gatcgggtct gctggccgag ctgctgacca cgcccggcgc ctcgggtacc gtcctggagg
      601 cggtggttcc gtacgcccag gcagcactgg ccgacctcct cggccgcgcg cccgaccagg
      661 cctgctcgcc gtccaccgcc cgctcccttg ccatgagcgc cttcgaccgc gcccgggatc
      721 tcggggcccg cgagcccttc gggctcggtt gcacggcgag cctggcaacc gaacggatga
      781 agcgcgggaa acaccgcgcc tgcatagccg tgcagacgac gaccagcacc tacagcgcca
      841 cgctgcagct ggaaggcgac cgggccagcg aggagcgcga tctggtggag ctcatctggc
      901 acgcgctcag ccggacgcta gagctgcacc tggagtcggc accgcccgat actttcgacg
      961 gcggtctgac cagcgcgctg gaacactggc gcgcgctggt gctcggtgag gagaccgccc
     1021 acgccaccgc accgcacgac ggccgtctgc tgctgcccgg ctcgttcaat ccgctccatc
     1081 gggcgcaccg ccggatgctc gagctggccg agcatctcac cggcctcgag ggggcatacg
     1141 agctgtcgat cgtgaacgtg gacaaaccgc tgctggacta cacggaaatc gaggagcggc
     1201 tcagccagtt cgaggcgccc gtctggctga cccgactgcc gactttcgtc gacaaggccc
     1261 gttacttccc gggcgctcat tttgccgtcg gcgtggacac gatcatccgc atcaccgaag
     1321 ccggctacta cggcagccag gccgaccggg acgcggccct tgacgagctt gccactctcg
     1381 gtacgcgctt tctcgtcttc ggcagaattc tgaacgacgc cttcctgacg ctctccgatc
     1441 tggacctgcc ggcgacgttc cgggcgctgt gccgcgaggt gaccgaggcg gagttcaacg
     1501 agccgatctc ctcaacggag atccggcgac gctagcccaa cggggccgct cagccgccct
     1561 cgagctcttc cacccgcttc ggccagttct ccttgagcag gcgggacagg gaccggtccg
     1621 ccagcaggcg cccgcccgtc tggcatcgag cgcagtagtt ggcctcgttc tcggcataca
     1681 cgatgcgctg cacgggagcg ccgcaaacgg gacacggctg gcgatacttt ccgtgcacgg
     1741 acatctccgg atggaaagcg gtcaccttcg acgggaagtc gtccccggcc gccgcccgga
     1801 gacggtccgt ccactcgcca agcacgccct tggttgcctc atggagccgc tggatctcac
     1861 cgtccgccat gtcccgcggg cgcttgaacg gcgacagcct tgcccgcagc aggatctcgt
     1921 cggagtaggc gttgccgatg cccgcaaaga cgtgctgatc cgtcagagcc cgcttcagcg
     1981 tgtggttgga ttcgcgcagt cgagacgcaa aggcggcaag atcggccgag aggacctcga
     2041 gaccgcccgg attcagcgct ccaagggcgt gggcgccgcg caccagccgc agcgacgccc
     2101 gcttcttgct gctggcttcc gtgagcagca ggctgcccgc gggaacgtcg aaggcggcca
     2161 gcgcgttggg ttttggcagg ggcgcgctcg gcttctgcca cttcagacgg cccgcgatca
     2221 tgaggtgaac gaccacgaag tgctcgccct ccagctccat gaccagctgc ttggcgagcc
     2281 gatggcaacc caagatacgc cggccgcaga cctcgtccac cgtcggttcg accgagcgga
     2341 gcacgaaagg gttggcaatg cggatggcct cgagcggctg gcccaccagt cgcctctcga
     2401 gatgctcgac gtagacggtc aggtcgggca gctcaggcac cgagggcctc cggttcccgg
     2461 tagcggatgg ccgttttcca gcggccgccg cggaaccgcg agatgagcag gacgcccttg
     2521 agcaggtagt cgccgatcag tgctgcgtac acccagaaca cgggcacgcc gagataggtg
     2581 gccagggccg cgagcccgca gcgcatgccg atgagcccga gaaaggtcgc catgagcggg
     2641 aagcgggtgt cgccagcacc cctgagcgcg ccgccgatgg tgaactcgac ggccagcagc
     2701 ggcatcatgg cgccgaggat gtaggtgaac tgcaccgtgt agtccacggt aaggggctcg
     2761 tcacctatga aaaagacggc cagctcctct gcatagaagc agacgacccc ccccaagcca
     2821 cccatggcga gcaccgcaag gcccgtggag cgccagccgc tgcgggcggc gccgtccgga
     2881 tcgttcgcgc cgaggtgctg gcccaccagc gtcgcgccgg cgatggagaa gccgaacccg
     2941 acggtcatgc acaccatgag catgttggcg ccgatgttgt aggcggcgaa agcttcggta
     3001 cc
//
LOCUS       DBNHOX010000325         2787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1119195, whole genome shotgun sequence.
ACCESSION   DBNHOX010000325 DBNHOX010000000
VERSION     DBNHOX010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1119195"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="AC16UZ_07100"
     CDS             complement(<1..516)
                     /locus_tag="AC16UZ_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246488.1"
                     /translation="MTQAEDKTGDQRGRAGRSQLVVILLVAGFSMAGAYLVFFLASSG
                     GVWGTTNNGEFVTPAMTMEALDLRDAAGTSATADGHWWLWTVAPGGCGEQCRFAVERL
                     RQVHALLNKDASRVRRALVTSTPGLELPPGLDVSDLERLVGPADGLASGIYIVDPIGN
                     LVLRYPLSDAGE"
     gene            complement(513..1205)
                     /locus_tag="AC16UZ_07105"
     CDS             complement(513..1205)
                     /locus_tag="AC16UZ_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial respiratory chain
                     complex IV assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HIR6246489.1"
                     /translation="MTLLVPLMLGLGFWQLDRADQKRAHQDTLFSRLASAPVAGRDTL
                     APEPFQRVRLEGRFEADRYFLVDNQVADGVVGYWVVQSFSTPQGVRWLVNRGWIAGGD
                     RRDQLPRVSAPTGTVVTVGVAWPDLGLVPLLDEDDWPLAWPVRVQRLDVARMAERLED
                     AVPVEIRLETAQPGALRAAPLTVGLQAERHEGYAAQWFALAGVLIVGYAVYGLRGAQR
                     ARRLNGRTDESR"
     gene            1317..1556
                     /locus_tag="AC16UZ_07110"
     CDS             1317..1556
                     /locus_tag="AC16UZ_07110"
                     /inference="COORDINATES: protein motif:HMM:NF022587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2909 domain-containing protein"
                     /protein_id="HIR6246490.1"
                     /translation="MIKIVIIFLLAGVVLSLFSSLAFLFRDSERPESKRALYALGIRI
                     TLAGALLLTVLYGFATGELSMGSNAPWHQSQTSGD"
     gene            complement(1563..2477)
                     /locus_tag="AC16UZ_07115"
     CDS             complement(1563..2477)
                     /locus_tag="AC16UZ_07115"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR6246491.1"
                     /translation="MASSSDQATYYIPHSGWYPIWVAVGSALLLFGLGSWLNDLKAET
                     EPSRALFYTGAVILSVVLFAWFAKVVNENLEGLTSPQLHRSYIWGISWFIFSEVMFFF
                     AFFGALLYARVLSVQWLGGEGAKGFTGEYLWPEFEAVWPVISNPDPAVFENPRGDMAS
                     PGLLSWGGYLPFWNTVILLTSSVTVHFAHTAIKNADRTRLLRWLGLTVALGVVFLFLQ
                     VEEYVHAYQELGLTLSSGIYGSTFFLLTGFHGFHVTLGTFMLFVQLVRAFRGHFQPKD
                     CFGFEASSWYWHFVDVVWVCLFLFVYVF"
     gene            complement(2506..>2787)
                     /locus_tag="AC16UZ_07120"
     CDS             complement(2506..>2787)
                     /locus_tag="AC16UZ_07120"
                     /inference="COORDINATES: protein motif:HMM:NF016337.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HIR6246492.1"
                     /translation="RVHPGEAKDVKFYVKNTTDRRMVGQAVPSVVPITATDYFHKTEC
                     FCFESQVLEPGEEMELPLRFIVDAGLPKNVQTISLSYALFDATGFAAAE"
BASE COUNT          554 a          953 c          854 g          426 t
ORIGIN      
        1 ttcgccagcg tcggacagcg ggtaacggag cacgaggttt ccgataggat ccacgatgta
       61 gataccggaa gccagcccgt ccgccgggcc gacgagcctc tcgagatcgg agacatccag
      121 ccccggcggc agctcgagcc ccggcgtcga ggtgaccagc gcgcgcctga ccctcgatgc
      181 atccttgttg agcagcgcgt gcacctgccg cagccgctcc accgcgaacc ggcactgctc
      241 gccgcacccg ccgggcgcga ccgtccacag ccaccaatgc ccgtctgccg tcgcggaagt
      301 gcccgcggcg tcgcgcaggt cgagggcctc catggtcatc gccggggtca cgaactcgcc
      361 gttgttcgtg gtgccccaga cgccaccgct gctggcgaga aagaagacca gatacgcccc
      421 cgccatggaa aagccggcca ccagcaggat caccaccagc tggctgcgcc cggcgcggcc
      481 tctctgatcc ccggttttgt cttcagcttg ggtcatcgac tctcatccgt tcgtccgttc
      541 aatcgcctgg cgcgctgcgc gccccgcagc ccgtagaccg cgtacccgac gatcagcacg
      601 cctgccaggg caaaccactg cgccgcgtaa ccttcgtggc gctccgcctg gaggccgacc
      661 gtcagcggcg ccgcacgcag ggcgccgggc tgggccgttt ccagccggat ttcaacgggc
      721 acggcatcct ccagccgttc agccattctt gcgacatcga gccgctgcac gcggacgggc
      781 caggccaggg gccagtcatc ctcgtccaga agcgggacca ggcccaggtc cggccaggcc
      841 acccccaccg tgaccaccgt gcccgtgggc gccgacaccc ggggcagttg gtcgcgacga
      901 tcgccgccgg caatccagcc gcggttgacc agccagcgca cgccctgcgg agttgaaaaa
      961 ctctggacca cccagtagcc caccacgccg tcagccacct ggttatccac cagaaaatag
     1021 cgatcggcct cgaaccgccc ttccagccga acccgctgga acggctccgg ggcgagggta
     1081 tcacggcccg ccacgggagc tgaggcaagg cgcgagaaca gcgtgtcctg atgtgcgcgc
     1141 ttctgatcgg cgcgatcgag ctgccaaaag cccagcccga gcatcagcgg gacgagcagc
     1201 gtcacggcca acgacatgcg ccaaccgggc gcccatctgc gcgcagcccc caacgcggtt
     1261 aatcccgccg gatggatgcg ttactctggc agcttctcgc gccgccggca tcctccatga
     1321 taaagatcgt gatcatcttc ctcctggcag gggtggtgct gagcctcttc agcagcctgg
     1381 ccttcctgtt cagggacagc gaacgcccgg aatcaaagcg agcgctttat gccctgggca
     1441 taagaataac cctggcgggc gcgctgctac tcaccgtcct gtacggcttt gccacgggag
     1501 agctctcgat gggcagcaac gccccgtggc atcagtccca gacctccggc gactgagcgg
     1561 cttcagaata cgtagacgaa gaggaacagg cagacccaga caacgtccac gaaatgccag
     1621 taccaggaag acgcttcgaa gccgaagcag tccttgggtt gaaagtgccc cctgaaggca
     1681 cgaaccagct gcacgaagag catgaacgtg ccgagcgtga cgtggaaccc atggaagccg
     1741 gtcagcagga agaaggtcga gccgtagatc ccgctggaca aagtcaggcc cagctcctga
     1801 tacgcgtgca cgtactcttc cacctgcaga aacagaaaga ccacgcccag cgcgacggtc
     1861 agcccgagcc acctcagcag cctggtccgg tcagcgttct tgatcgccgt gtgggcgaaa
     1921 tgcaccgtga cggaagaggt gagcaggatg accgtgttcc agaacggcag gtagccgccc
     1981 cagctcagga gcccggggga ggccatatca ccccgggggt tctcgaacac cgccgggtcg
     2041 ggattgctga tcaccggcca gacggcctcg aattcgggcc agaggtactc cccggtgaat
     2101 cctttcgcgc cctcgccgcc gagccactga accgagagca cccgggcata gagcagcgcg
     2161 ccgaagaagg cgaagaagaa catcacctcg gagaagatga accagctgat gccccagatg
     2221 taagaacgat gaagctgggg gctcgtgagg ccctcgagat tctcgttgac cacctttgca
     2281 aaccaggcga acagcaccac cgacaggatg acggccccgg tatagaacag cgcgcggctc
     2341 ggctccgtct cggccttgag atcgttcagc caggagccca gcccgaacag aagcagggcc
     2401 gacccgacgg ccacccagat cgggtaccac ccgctgtggg ggatgtaata agtggcttgg
     2461 tctgatgagc tggccatggc cgttctctcc cttttaggtg tcgggttact ccgccgccgc
     2521 gaaaccggtg gcgtcgaaca gggcgtagct gagcgatatg gtctgcacgt tcttcggcag
     2581 ccccgcatcg acgatgaatc gcagcggcag ctccatttcc tcgccaggct ctaagacctg
     2641 gctttcgaaa cagaagcatt cagttttgtg aaaatagtcg gttgccgtga tcgggaccac
     2701 cgaggggacc gcctggccca ccatgcgccg atcggtggtg ttcttgacgt agaacttcac
     2761 gtccttggcc tcaccaggat gcacgcg
//
LOCUS       DBNHOX010000326         4483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1311755, whole genome shotgun sequence.
ACCESSION   DBNHOX010000326 DBNHOX010000000
VERSION     DBNHOX010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1311755"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1118
                     /locus_tag="AC16UZ_07125"
     CDS             228..1118
                     /locus_tag="AC16UZ_07125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6246493.1"
                     /translation="MVIVNWNSGDMLATCLDGLAAHCANVIGQVIIVDNASTDSSLDF
                     PMPTSLEVDLIRSGCNLGFAAACNLGASRSGADFLLFANPDLVIGQDILTQPLRRLRG
                     DATIGAVGIGLYDAGSGQRQRTCCRLPTPISMLARSLGIDRLWPRLGYRLSGWDHSSS
                     RKVDHVIGACYLTPRAVFDRLGGFDERFFLYLEDLDFSARIQQAGMDILYLANPCASH
                     VGGGSSRQIKDERLFYMLRSRLQYVHKHMGVWGLIIVGFSTLAIEPWLRILASVLAGS
                     VTGCGEVLRGYGRLFRWLGS"
     gene            1115..2185
                     /locus_tag="AC16UZ_07130"
     CDS             1115..2185
                     /locus_tag="AC16UZ_07130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6246494.1"
                     /translation="MIRVLALTRYGALGASTRLRTLQYVPLLREDGIDIDVSPLLVDA
                     YLEGLYRRGRGPLLASLAGYTRRLAALTNQRAYDLVWLEKELLPWLPAAFERMLRRPY
                     LVDYDDAIFHRYDQHPRALVRWLLGNKIDLVMRRAQVVVVGNRYLADAARRAGARELE
                     IVPTSVDTQRYRPLAKDSGRNASGDFNVGWIGTPQTAPFLKTLTPVFERLHGSGGFRF
                     TFIGVPAGLDVGVPYEAFPWTESDEVSLLQRLDCGIMPLPDLPFERGKSGYKLIQYMA
                     CGLPVVASPVGENQHIVREGVNGFLASQTDEWIAALQGLRDAADRGRALGMAGREIVE
                     ATYDCRRNAKRIAALLRAAVAQ"
     gene            complement(2289..4031)
                     /locus_tag="AC16UZ_07135"
     CDS             complement(2289..4031)
                     /locus_tag="AC16UZ_07135"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6246495.1"
                     /translation="MYDHLIRNATIVDGLGGKPCTGDIAIKDGVIAAVGEVSGNARET
                     LNAEGAYATPGWVDVHTHYDGQVSWDDTIEPSFSQGVTSIVMGNCGVGFAPCRPGDEK
                     RVIELMEGVEDIPGTALYEGIEWGNWESFPEYIEYLSTKRYTMDIGTQIPHSALRNYV
                     MGERALTHEDATAEDLEAMCRLVQDGLAAGALGFSTSRTIGHRSVDGEPIPGTFAEKD
                     ELLAIARAMREAGRGVFQAVPAGVVGDIAGPERWTTEQEVELFAEVARASGRKCTFTL
                     IQNGNRPDQWRNCLNIVEQANAEGIELTPQIATRPIGIVTSLDSYHMFMRRETYLKYA
                     DLPLSSRLAELRKPEVRAAILADRDVPPDQPGVMANVFALMQMAAPAMFPIDIPIDYE
                     PEPSANMGARAAAAGMDIQAFMYDFLTAADGDQFAILLGANFADGTFSVIEEMIPHPN
                     TTIGLSDAGAHVNLIFDAVGPTYQLTHWVRDRSRGRRFPIELMVHKQTYSNARLFGLN
                     DRGSIEVGKRADINVIDLERLGLGKLHVHRDLPAGGSRILQPAHGYVGTFVAGVKTRE
                     NDRDTGARPGRVIR"
     gene            4150..>4483
                     /locus_tag="AC16UZ_07140"
     CDS             4150..>4483
                     /locus_tag="AC16UZ_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246496.1"
                     /translation="MTSSIQTGAVLVVFGLAIGAASASAEVCDVDQVLQRLQSEVLGA
                     LSEEQQTRAREVLGEFCGGMISESPRRVVSTADPTEEAASKAPGELQEAEESEGTLKV
                     LGVEFQKAD"
BASE COUNT          734 a         1344 c         1490 g          915 t
ORIGIN      
        1 gaagcggtcc ggcgctggcg gctggcatgt ctggccctct cccggtcgcc tggcgacgag
       61 cgggtcgcaa atcaggatga caacgctatc atttatccat ggcgattgct gaggaaaagg
      121 tcgggagagg gtgcgcgccc gggagtgcgt gtcgctagaa tgagctgagt cttgtggggt
      181 tgcgcctgtc gttggatacc tgctttgcaa cagatcggag cgttgacgtg gtcatcgtca
      241 actggaattc cggtgacatg ctcgccacct gcctcgatgg cctggcagcc cactgcgcga
      301 acgtaattgg tcaggtcatc atcgtggaca atgcatccac ggattcttct ctggactttc
      361 cgatgccgac ctcattggag gtcgatctga tccgctccgg gtgcaacctt ggattcgcag
      421 ccgcctgcaa tctcggagcg tcgcggtctg gggcggattt cctgctgttc gcgaatccgg
      481 atctggtcat cggccaggac atattgaccc aaccgctgcg ccggctccgt ggggacgcca
      541 ccattggcgc ggtgggcatt ggtttgtatg acgctggttc aggccagcgc cagcgaacgt
      601 gctgccggtt accgacgcca atcagcatgc tcgcgcgttc tcttggcata gatcgtctct
      661 ggccgcgact gggctatcgt ctgagtggat gggatcattc cagctcgcgc aaagtcgacc
      721 acgtgatcgg ggcctgctac ctgacgccac gcgctgtatt tgatcgcctc ggcggattcg
      781 acgagcgatt tttcctctac ctcgaggatc tggacttttc ggcgcgaatt cagcaggccg
      841 ggatggacat tctctatcta gccaatccct gcgccagcca cgtcggaggt ggttcatccc
      901 gacagatcaa ggacgagagg cttttctaca tgttgaggtc gcggttgcag tatgtgcaca
      961 agcatatggg cgtgtggggc ctgatcatcg ttgggttcag cacgctcgcg atcgagccgt
     1021 ggttgcggat tctggccagc gtgctggctg gttcggtaac gggttgtggc gaggtcctgc
     1081 gtggctacgg gcgactattt cgttggctgg gctcgtgatc cgcgttctcg ccttgacgag
     1141 atatggcgcg cttggagcca gcacccggct gcgaacgctg caatacgttc cgcttctgcg
     1201 tgaggacggc attgacatag atgtttcgcc tctcttggtc gacgcctatc tcgaggggct
     1261 gtatcgccgc gggcgggggc cgcttctggc ttccctcgcc ggctacacgc gccggctcgc
     1321 tgcgttgacc aaccagcgtg cctacgacct ggtatggctg gagaaggaac tgctgccctg
     1381 gctccccgca gcgttcgaac ggatgttacg gcggccgtac ctggtcgact acgacgacgc
     1441 gatttttcac cgctacgatc agcacccgcg ggcgctggta cggtggctgc tcggaaacaa
     1501 gatcgatctc gtgatgcgtc gcgcgcaagt cgtggtggtt ggcaatcgtt accttgccga
     1561 cgccgcgcgc cgtgccggtg cccgtgaact cgagatcgtc ccgacctcgg tggatacgca
     1621 gcgataccgg ccgctggcga aggattctgg aagaaacgca tcgggtgact tcaacgtggg
     1681 ttggatcggg acgccgcaga cggcaccgtt tctgaagaca ttgacccccg tgttcgaacg
     1741 gctgcacgga tcgggcgggt tccggtttac gtttatcggc gtgccagccg gtcttgacgt
     1801 cggggtgccc tacgaagcgt tcccctggac ggagagtgac gaagtcagcc tgctgcaacg
     1861 gcttgattgc ggaatcatgc cgcttccgga cctgcccttc gagcgcggca agtcgggtta
     1921 caaactgatc cagtacatgg cctgtgggct cccggtggtt gcaagtccgg taggggaaaa
     1981 ccagcatatc gtccgcgaag gtgtgaacgg ctttcttgcc tcgcaaaccg acgagtggat
     2041 cgcggctttg cagggtctgc gcgatgcggc tgatcggggt cgcgccctcg ggatggcagg
     2101 cagggaaatt gtggaggcga catacgactg ccgacgcaac gcaaagcgga tagcggcctt
     2161 gttgcgtgct gccgttgctc aatgaatccg gtatcgagcc gctttgcggg tctcaacccg
     2221 ggtttttcct gtcgcacgcc tgctctattg gcaggaccag cagctccgga agaccaccgc
     2281 tagcgtagct atcggatcac ccggccaggc cgcgcgccgg tgtcgcgatc gttttcccgg
     2341 gttttcactc cagccacgaa cgtgccgacg tagccgtggg cgggctgcag gatgcggctg
     2401 ccgccggccg gcagatctcg atgcacgtgc agcttgccga ggcccaaccg ctccaggtcg
     2461 atgacgttga tgtcggcccg cttgccgacc tcgatgctgc cccggtcgtt caggccgaac
     2521 agcctggcat tgctgtaggt ctgcttgtgc accatcagct cgatgggaaa ccgtctgccc
     2581 cggctgcggt cgcgcaccca gtgtgtgagc tggtaggtgg gccccacggc gtcgaagatg
     2641 agattgacgt gcgcacccgc atcggaaagg ccgatggtcg tgttggggtg gggaatcatc
     2701 tcctcgatca cgctgaaggt accgtcggcg aagttcgcgc cgagcagaat ggcgaactgg
     2761 tcgccgtctg cggcggtgag aaaatcgtac atgaaggctt ggatgtccat gcccgcagcg
     2821 gccgcacgcg cgcccatgtt tgccgacggc tccggctcgt agtcgatggg gatgtcgatg
     2881 ggaaacatgg ctggtgcggc catctgcatc agggcgaaga cgttggccat cacgcccggc
     2941 tggtccggcg ggacgtccct gtctgccagg atcgcggccc tcacctcagg ctttctcagc
     3001 tccgcgagcc gcgaagacag gggcaggtcc gcatacttca gataggtctc ccggcgcatg
     3061 aacatgtgat aggagtccag gctcgtgacg atgccgatgg ggcgtgtcgc gatttgaggc
     3121 gtgagctcga tgccctcggc gttggcctgc tccacgatgt tcaggcagtt gcgccactga
     3181 tcgggcctgt tgccgttctg aatcagggtg aacgtgcact tgcggccgct ggctcgggcc
     3241 acctcggcga aaagctcgac ttcctgctcc gtcgtccatc gctccggccc tgcgatgtcg
     3301 ccgacgaccc cggccggcac ggcctggaag acgccgcgtc cggcctccct catggcccgg
     3361 gcgatggcca gcagctcgtc tttctcggcg aacgtcccgg ggatgggctc gccgtcaacg
     3421 gaccggtggc cgatggttct cgaggtagag aatcccaggg ctccggcggc gagaccgtcc
     3481 tggaccagcc ggcacatggc ttcgaggtcc tcggcggtgg catcctcgtg ggtgagtgcc
     3541 cgctcgccca tcacgtagtt acgcagagcg ctgtgcggaa tctgggtgcc gatatccatg
     3601 gtgtaccgct tggtcgaaag gtactcgatg tactcgggga acgattccca gttgccccac
     3661 tcgatgccct cgtacagtgc ggtgccgggg atgtcctcga ccccctccat cagctcgatc
     3721 acgcgctttt cgtcccccgg gcggcacggc gcgaagccga cgccgcagtt gcccatgacg
     3781 atgctggtca caccctggct gaacgacggc tcgatggtat cgtcccagct gacctgaccg
     3841 tcgtagtggg tatgcacgtc cacccagccg ggagtggcgt aggcgccctc cgcgttcagg
     3901 gtctccctgg cgtttccgga gacttcgccc accgcggcga tgacgccatc cttgatggca
     3961 atgtcgcccg tgcatggctt tccgccgaga ccgtcgacga tggtggcgtt cctgatcagg
     4021 tggtcgtaca tggtggctcc tcgagcaata ccgtgcccgt cgtggtgact gtgggcgtaa
     4081 cgaagaaaag ggtggcctct cgtgcacgaa cggcagcacg tctgagccgc ggtggtgcat
     4141 aatgccgcca tgaccagctc gattcaaacc ggggccgttc tcgtcgtctt cgggctcgcg
     4201 atcggcgcgg cttcggcaag cgcagaggtt tgcgatgtcg atcaggtact acagcgcctg
     4261 caatccgaag tgctgggcgc gctgtcggaa gaacagcaga cgcgagcccg cgaggtgctt
     4321 ggggaattct gcggcggcat gatttcggaa agccccagac gggtggtcag cacggccgac
     4381 ccgaccgaag aggctgcctc caaagcgccc ggggagttgc aggaagccga ggagtcggag
     4441 gggacgttga aggtgctcgg ggtggaattt cagaaggccg acg
//
LOCUS       DBNHOX010000327         2042 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_990680, whole genome shotgun sequence.
ACCESSION   DBNHOX010000327 DBNHOX010000000
VERSION     DBNHOX010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_990680"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2008
                     /locus_tag="AC16UZ_07145"
     CDS             <1..2008
                     /locus_tag="AC16UZ_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246497.1"
                     /translation="HQVEHWMLDRQRNISDLWQCIDPANDAATTSSALGVVEEPVDPE
                     APALPEIPEELAAPAEEEVPEDDELAAEELPAEEPEEEEKAASVKLAIRGLAFSPGGH
                     KREAALDRAKEEELLLRAAAAQAKGTAGYLGDEGELHPQKGLTVTPSRIADVNALTGA
                     RVMARRAALHEKGENPINPFGGMLTPHEAEGPGGSWWGHGRVQKTAAPITNQAELDAA
                     VRDQVLKGQLSAVQGAVRGQSMARHQRGERMGDVAGRLGGLAAGGLVGKRLGGGLGAL
                     GGAGLGMLAGGKLGKEVGKEVDVVREHKLASVAERMAQRVKLAQEPAAAEAPMASPTD
                     NTELQPANYLQAEQMGQAAQSANEINFFRQKAQAAMQQAMQAVQQSQMQGEQLQGQMM
                     ELQQRADAADASVREALSEATSARDEALSQTQQAANMRMGMQKLRQQMIEVASQDPAA
                     VAAAEMDAVAQGSKNQVAMQAQEQAAAEAADAGMVPPPGPEGPAGQAPSPATPPGAAP
                     AAGAPDMNANAGGPPGAAAGGGPGGAQEPQSQVAPKQASVKTAGLLGAGAGAAIGGGL
                     GLKRSAELAGSDPQALRDRVQVLEETQDGSYGQAANLAKAKKTLADSELAQAHPRGFG
                     LRATARGAALGGIRGSAVETAVRQGVNPAAVLNTQRQAGRLANP"
BASE COUNT          363 a          643 c          786 g          250 t
ORIGIN      
        1 ccaccaggtg gagcactgga tgctcgaccg gcagaggaac atctctgacc tctggcagtg
       61 catcgacccg gccaacgatg cggcgaccac gagcagcgcg ctcggggtgg tcgaggagcc
      121 tgtcgaccca gaggcccccg cgctccccga gattccggag gagctcgccg caccggcgga
      181 ggaggaggtc ccggaggacg acgagctcgc cgccgaggag ctccctgcag aagagccgga
      241 agaggaggag aaggctgctt cggtgaagct ggctattcgg ggattggcgt tctcccctgg
      301 tggccataag agggaagcag ctctcgatag ggcaaaagag gaggaattgc tgctgcgtgc
      361 tgccgctgcg caggccaagg ggactgcggg gtacttgggc gacgagggtg agttgcaccc
      421 tcagaagggg cttacggtta ctccctcccg tatcgctgac gtgaacgccc tcacgggggc
      481 cagggtgatg gcacgtcgag ccgctcttca cgagaagggt gagaatccaa tcaatccatt
      541 cggcggtatg ctcactccgc atgaagcgga gggccctggg ggtagttggt ggggccatgg
      601 gcgcgtgcag aagacagccg cgcccatcac gaaccaggcc gagctcgacg cagcagtccg
      661 ggaccaggtc ctgaagggtc agctctcggc cgtacagggc gccgtgcgcg ggcagagcat
      721 ggcccggcac cagcgcggcg agcgcatggg agacgttgca gggaggctcg gaggcctcgc
      781 tgcgggcggc ctcgtcggca agcgcctggg tggcggcctt ggcgcgctcg ggggtgcggg
      841 gctcggcatg ctggctggcg gaaagctggg caaggaggtc ggcaaggagg tcgacgtcgt
      901 tcgggagcac aagctggcgt ccgtcgccga gcggatggcg cagcgcgtga agctcgccca
      961 ggagccggcg gcagccgagg ccccgatggc ctccccaacg gacaacaccg agctccagcc
     1021 tgcgaactat cttcaggctg agcagatggg tcaggcggcc caaagtgcca acgagatcaa
     1081 cttcttcagg cagaaggctc aagcggccat gcagcaagcc atgcaggccg tgcagcagag
     1141 ccagatgcag ggagagcagc ttcagggcca gatgatggag ctccagcagc gtgcggacgc
     1201 ggccgacgcc agcgtgcgcg aggccttgag cgaggcgacc tcggcgcgcg acgaagcgct
     1261 ttcgcagact cagcaggcgg ccaacatgcg gatgggcatg cagaagctcc gtcagcagat
     1321 gatcgaggtc gcgagccagg acccggcggc cgtggcggcg gcggagatgg acgctgtggc
     1381 ccagggcagc aagaaccagg tcgcgatgca ggcccaggag caggcggcgg cagaggcggc
     1441 ggacgccggc atggtgcccc ctcctggtcc cgaagggccc gcggggcagg ctccgtctcc
     1501 cgctacgcct ccgggcgcgg cgccggcggc gggcgccccc gacatgaacg cgaatgccgg
     1561 ggggccgcca ggggcggcgg caggcggcgg tcccggcggg gcccaggagc cccagagtca
     1621 ggtggccccc aagcaggcct cggtcaagac ggcgggcctc ctcggtgcgg gcgcgggcgc
     1681 tgccatcggt gggggtctcg ggttgaagcg gtcggccgag ctcgccggct cagaccccca
     1741 ggctctccgg gatcgagtcc aggtgctcga ggagactcag gacgggtcgt acgggcaggc
     1801 cgccaacctc gccaaggcga agaagaccct cgcggactcc gagctcgccc aggcgcaccc
     1861 ccgggggttc gggctgcgcg cgacggcgcg cggagcggcg ctcggaggca tccgagggag
     1921 cgccgtcgaa acggctgtgc ggcaaggagt caacccggct gctgtgctca acacccagag
     1981 gcaggccggg cgcctggcca acccgtagga gaagaacatg ctcgacgcat ttgcatcggt
     2041 cc
//
LOCUS       DBNHOX010000328         4057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1697380, whole genome shotgun sequence.
ACCESSION   DBNHOX010000328 DBNHOX010000000
VERSION     DBNHOX010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1697380"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..941
                     /gene="rsmI"
                     /locus_tag="AC16UZ_07150"
     CDS             123..941
                     /gene="rsmI"
                     /locus_tag="AC16UZ_07150"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR6246498.1"
                     /translation="MSGVLYVVATPIGNLDDITRRGADVLASVQIVAAEDTRRSQVLL
                     AAIGATQAELVSFHAHNEQNVARRLMAALQAGGDVALVSDAGTPLVSDPGFDLIRRCW
                     EEGVRVVPVPGASSVLAALSVCPLPAGRFFFEGFLPARAGQRRERLRHLAGRTEAVVF
                     FEAPHRVAACLADLEQLVPQRRLMVGREMTKRHESLHCGTPGELREALSSTLRGEFVF
                     VMEAAAGAGLDADAAEVMEILCAELAPAQAARLGSRLLKMDRSVLYRIALEQRS"
     gene            982..1360
                     /gene="rnpB"
                     /locus_tag="AC16UZ_07155"
     ncRNA           982..1360
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC16UZ_07155"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1677..2135
                     /gene="mraZ"
                     /locus_tag="AC16UZ_07160"
     CDS             1677..2135
                     /gene="mraZ"
                     /locus_tag="AC16UZ_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR6246499.1"
                     /translation="MFRGINSATLDGKGRMALPTRHRESVVAVSEGRLVVTIDMREKC
                     LLMYPLPEWEVVQRKLESLSNIGEQARLLQRLLIGHATDLELDGQGRVLLPQMLRGYA
                     ALEKKLVLVGQGNKIEIWSDSLWQSRMNTWLSDGAGELLANGDEFTGLSV"
     gene            2132..3064
                     /gene="rsmH"
                     /locus_tag="AC16UZ_07165"
     CDS             2132..3064
                     /gene="rsmH"
                     /locus_tag="AC16UZ_07165"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR6246500.1"
                     /translation="MSNTHEPVLCQETVATLTGMGNSTVRQGVYVDATFGRGGHSRAL
                     LGVLGDESRVVALDRDPAAVAAGREMASSEPRLTICHARFSDLRRALASLDIESVQGV
                     LMDLGVSSPQLDDPQRGFSFLRSGPLDMRMDPGAGMSAAQWLNSASEQEIAGVLRSLG
                     EERYARRIAAAIAAARPISDTGELVALVRAAQPRATPGKHEATRVFQAVRMHVNDELG
                     ELESGLRQAFEMLSAGGRLAVIGFHSLEDRMVKRFFKSLTEPPPLPRRLPVRAAAQVV
                     PARSVAGPVRAGAAERARNPRSRSAVLRVVEKIS"
     gene            3061..3327
                     /gene="ftsL"
                     /locus_tag="AC16UZ_07170"
     CDS             3061..3327
                     /gene="ftsL"
                     /locus_tag="AC16UZ_07170"
                     /inference="COORDINATES: protein motif:HMM:NF016860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HIR6246501.1"
                     /translation="MSRLYGWIVATLLVAVTVSGVQVAFSSQKVRQLHADLQAAQRVQ
                     DEELAEHSRLLLERSAQTSYVNVERVAEAELGMIFPDRVEPIEP"
     gene            3324..>4057
                     /locus_tag="AC16UZ_07175"
     CDS             3324..>4057
                     /locus_tag="AC16UZ_07175"
                     /inference="COORDINATES: protein motif:HMM:NF015662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR6246502.1"
                     /translation="MKGWRHYTLVGGFALGCVAVIVRLGYLNWTEREFLQEQGDARSV
                     RSEVLPAYRGVVYDRHGEPLAVSTPVFAVWLDPSRAGLTDESLGALAELLELDPARLS
                     ASVQSNGHREFLYLKRRVPWDRAQAVRELGISGVHLQSEYRRYYPASETTSHVVGTTG
                     IEDSGLEGIELSFDGRLRGEHGRKIVLKDQRGETIKDLDYVASPKFGDDLNLSLDLRL
                     QFLAYRELKSAVVGHRATSGSLVMLDA"
BASE COUNT          663 a         1229 c         1406 g          759 t
ORIGIN      
        1 cgggagatga gggggagact tcgggcacgt tcgcttcgat tgtctcgagc ggcgtgcttt
       61 gacaggcgga cactgcgaga atagcgaacg cccaagccca ggcccttatc cggtttgctg
      121 ccatgtctgg tgttctctac gtcgttgcca cgccaattgg aaacctcgac gacatcactc
      181 gtcgaggcgc cgatgtcctg gcgtcggtgc agatagtcgc cgccgaggat accagacgct
      241 ctcaggtgct gttagcagca atcggcgcaa cccaggccga gctggtctcg ttccacgcgc
      301 acaacgagca gaacgtggcg cggcgcctca tggccgcgct gcaggcgggt ggcgatgtgg
      361 cactggtctc cgacgccggc acgcccctgg tatccgaccc gggcttcgat ctcatccggc
      421 gctgctggga ggaaggcgtc cgggtggttc ccgtccccgg cgcgagctcg gtcctggctg
      481 ctctgtccgt ctgtcctctt ccggccggcc gctttttctt cgagggcttt ctgccggcaa
      541 gggcggggca gcgccgcgag cggctgcggc atcttgccgg acgcacggaa gccgtcgtct
      601 tcttcgaggc gccccaccgg gtcgccgcct gccttgcgga tctggagcag ctggtcccgc
      661 agcggcgact catggtcggg cgggaaatga ccaaacgtca cgagagtctg cactgcggca
      721 ccccgggaga gctgcgcgag gcgctgtcga gcacccttcg gggggagttc gtcttcgtga
      781 tggaggccgc cgcgggagct ggcctcgacg cggacgccgc cgaggtcatg gagatccttt
      841 gtgccgagct ggctccggct caagccgcac ggctcggctc gcgcctgctc aagatggacc
      901 gctccgtgct ctaccggatc gccctggagc agcgctcctg agggcctggc aatccgccgg
      961 tccggggatt agtcttgccc tcgagtcggc cagacagtcg ctggcagcat cagctgcgag
     1021 aggaaagtcc gggctccaca ggacgaggtg ccaggtaacg cctggggggc gcgagcccac
     1081 ggaaagtgca gcagaaagca aaccgcctaa ggcgcggcgc tccggcgctg cgaacggtaa
     1141 gggtgaaagg gtgcggtaag agcgcaccgc gcgcgtggta acactgcgcg gctaggtaaa
     1201 ccccgcctgg agcaaggcca aataggaacc ccatggtgtg gtccgcaccg ggttcgggta
     1261 ggtcgcacga ggcgttcggt gacgaacgcc ctagatgaat gactgtcggc tgcgttctcg
     1321 gacgcggcaa cagaacccgg cttatcggcc gactcgtttc ttttcgcacc taaaacggga
     1381 tttttaatgc cgattgggtc tagaagttaa ggtgaattct agatacctgc ttctacctga
     1441 ggttaactaa gaagataaag ctgcccgctg ccttctgaca gcctctctgc gccgaaaaaa
     1501 tccataaatt tccgtaggtt atcgacgttt tcctcgcttg acaggcaagc ctgtgggtcg
     1561 atatcgtggc tggtggtgtg ccaatttggg atttagtggg agattgtggg agattccgtc
     1621 tgaatcggtc gtgacctggt tcattttgac cgattcgcgc ggcccccagg gcagtcatgt
     1681 ttcgaggcat caacagcgcc actctggatg gcaaggggcg catggctctg ccgacccgtc
     1741 atcgcgagtc ggtggtcgcc gtgagcgagg ggcgccttgt cgtgaccatc gacatgcggg
     1801 aaaagtgcct gctgatgtac ccgcttccgg aatgggaggt ggtgcagcgc aagctcgaaa
     1861 gcctttcgaa catcggtgag caggcccggt tgctgcagcg gctgctgatc ggccatgcca
     1921 cggacctcga gctggacggc cagggtcggg tgctgctgcc gcagatgctc agaggctatg
     1981 cggcattgga aaagaagctg gtgctggtcg gtcagggcaa caagatagaa atttggagcg
     2041 attcgctgtg gcagagccgg atgaacacct ggctctcgga tggggctggg gaactgctgg
     2101 ccaacggcga cgagttcacc ggattgtcgg tttgagcaat acgcacgagc cggtgctttg
     2161 ccaggagacg gtggcgacat tgacggggat ggggaactcg acagtcaggc agggcgtgta
     2221 cgtcgacgcc acgttcgggc gcggtggcca ttcgcgcgcg ctcctggggg ttcttggtga
     2281 cgagagccgc gtggttgccc tggatcggga cccggcggcc gtcgcggcgg gaagggagat
     2341 ggccagctcc gagccccgtt tgaccatttg tcacgcgcgc ttttctgatc tgcgccgggc
     2401 gcttgcctcg ctcgacatcg agtcggtgca gggcgtgctc atggatctcg gcgtttcctc
     2461 tccccagctg gatgatcctc agcgaggttt cagctttctg cggtccgggc ccctggacat
     2521 gcgcatggac ccgggcgcgg gaatgtctgc cgcgcagtgg ctcaattcgg cttcggagca
     2581 ggagatcgcc ggcgtgctgc gatcgctcgg agaggagcgc tatgccaggc gcatcgccgc
     2641 cgccatcgcg gcggcccggc caatctccga taccggtgag ctggtggcgc tggtgcgcgc
     2701 ggcgcagccg cgagccacac ccggcaagca tgaggccacc cgcgtgttcc aggcggtgcg
     2761 gatgcacgtc aacgatgagc ttggcgagct cgagagcggg ttgcgacagg cattcgagat
     2821 gctatctgcc ggggggcgcc tggcggtgat cggctttcac tctctggaag accggatggt
     2881 caagcgcttc ttcaagagct taacggagcc cccgccgttg ccgcgccggt taccggtaag
     2941 ggcggcggct caggtcgtcc cggcgcgctc ggtggcaggg cccgtccgcg ctggcgctgc
     3001 cgaacgggcg cgcaacccgc gctcgcgcag cgccgtcctt cgggtggtgg agaaaatctc
     3061 atgagtcggc tttacggctg gatcgtggca accctgctgg tcgcggtcac ggtgtccggc
     3121 gtgcaggttg cgttcagcag ccagaaagtg cggcagcttc acgccgatct tcaggccgcg
     3181 cagcgtgttc aggacgagga actcgccgag cacagccggc tgctcctgga gcgcagcgcg
     3241 cagacctcat acgtgaacgt cgaacgggtg gcggaagccg agcttggcat gatcttcccc
     3301 gaccgggtgg agcccatcga gccatgaagg gctggaggca ctacacgctg gtgggaggct
     3361 tcgctctggg ctgcgtggcc gtgatcgttc ggctcggtta cctcaactgg accgaacggg
     3421 agttcctgca ggagcagggc gatgccagat ccgttcgcag cgaggtgctg ccggcctatc
     3481 gcggtgtcgt ctacgaccgc catggcgagc cgctggcagt cagcaccccc gttttcgcgg
     3541 tctggttgga tccaagccgt gctggcctga cggacgagtc gctgggcgcg cttgccgagt
     3601 tgctcgaact ggaccccgct cgcctgtccg cgtccgtgca gagcaacggc catcgggagt
     3661 ttctctacct caagcgccgg gtcccctggg accgggcgca ggccgtcagg gagctgggca
     3721 tctcgggcgt tcacctgcag tccgagtatc gccgctacta cccggccagc gagacgacgt
     3781 ctcacgtggt cggcaccacc ggcatcgagg attccgggct ggaggggatc gagctctcct
     3841 tcgacggccg gcttcgcggc gagcacggac ggaagatcgt cctgaaggat cagcgcggcg
     3901 agaccatcaa ggatctcgat tacgtcgcct cgccgaaatt cggcgacgac ctgaacctga
     3961 gcctcgatct gaggctgcag tttctcgcct atcgggagct caagtccgcg gtggtgggtc
     4021 atcgtgccac gtcgggctcc ctcgtcatgc tcgatgc
//
LOCUS       DBNHOX010000329         7921 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_797213, whole genome shotgun sequence.
ACCESSION   DBNHOX010000329 DBNHOX010000000
VERSION     DBNHOX010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7921)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7921)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7921
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_797213"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="AC16UZ_07180"
     CDS             complement(<1..703)
                     /locus_tag="AC16UZ_07180"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013179.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR6246503.1"
                     /translation="MTQPHGNTAYNMPSLAKSDILDQLTGAVIVCDGSLRVCYMNPAA
                     ESLLNTSEHQSTGVQLAELLFDAAASPFADLAGIFTTEQSITKRAAQFQLRDGTEVIA
                     DLTASLEPNSGHLVIELQPLNRLLRINRDEHSFFSQQTTRTLIRGLAHEVKNPLGGLR
                     GAAQLLQRELDASELTEYTRIIIEEADRLTQLVDDMLGSNRQPEYADVNIHELLEHVV
                     RLVDAELGGRVKFARD"
     gene            complement(960..1577)
                     /locus_tag="AC16UZ_07185"
     CDS             complement(960..1577)
                     /locus_tag="AC16UZ_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR6246504.1"
                     /translation="MQLLATLKRIAFLSIISTPWLMLSTQSMAGTTIYKTVDEDGNVV
                     FTDTPPRADGPSETITLTSPNSFDPAEAGTEGSGRQLWIVDPDAEGEASGEFYGALTV
                     TSPADDESIRENAGNVTVTVKLEPPNLQPGHRIRLLLDGSPTGGTNTTGRFALSNVDR
                     GTHSLKAEVVDDAGKLIYAGPSSTFHLQRYSVLNAPKRPVNPINN"
     gene            complement(1691..3103)
                     /gene="glnA"
                     /locus_tag="AC16UZ_07190"
     CDS             complement(1691..3103)
                     /gene="glnA"
                     /locus_tag="AC16UZ_07190"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR6246505.1"
                     /translation="MAENTLKLIKDNDVKWVDLRFTDPKGKEQHTTIPAHRLDADALN
                     DGFMFDGSSISGWKSINESDMILMPDDSTPVIDPFRDDTTLNLRCDIIEPSTMQPYDR
                     DPRSLAKRAEAYLTSTGIGDTAYFGPENEFFVFDDVRWKVEMSGASYSIGAEEAAWES
                     HSEFEGGNLGHRPSVKGGYFPVPPVDSAADLRSAMCSAMMAMGLDVEVHHHEVATACQ
                     NEIGARFNTLVRKADEVQIYKYCVHNVADAYGKTATFMPKPLVGDNGNGMHVHQSIAK
                     GDTNVFHGDEYAGLSKTALYYIGGIIKHARAINAFANASTNSYKRLVPGFEAPVLLAY
                     SARNRSASIRVPYIQGGNPRAYRVEVRFPDSTGNPYLTFAAMLMAGLDGIQNEIHPGD
                     SFDKDLYDLPAEELEGVPTVCSSLEQALQALDEDRDFLKAGGVFSDGMIDGYLALKQQ
                     EVDYLNSTTHPAEFEMYYSV"
     gene            complement(3303..3587)
                     /locus_tag="AC16UZ_07195"
     CDS             complement(3303..3587)
                     /locus_tag="AC16UZ_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR6246506.1"
                     /translation="MSELETLDARGLLCPLPVIRTQEKVKGLPPGTVVEIVATDPGVM
                     QDIPAWCRVHGHELISMTEGEEEAAAEGKADADRLIRGGREIRVRIKVRQ"
     gene            complement(3574..4656)
                     /locus_tag="AC16UZ_07200"
     CDS             complement(3574..4656)
                     /locus_tag="AC16UZ_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246507.1"
                     /translation="MPRPKRWRIMAEQRRAVALISGGLDSMLAAKVVMEQGVHVEGVN
                     FFTGFCVEGHTHAIREKDRNKPKRNNALWVAEQLGIRLHIIDVIDEYKDVLLNPKHGY
                     GAHLNPCLDCKVFMVRKALAWSFMEENGFDFIVTGEVIGQRPKSQRKETMPIIARESG
                     ADDRLLRPLCAKHLPPTLPEREGWVDREALYDFHGRNRKPQFALARRFGFEDWSQPAG
                     GCCFLTNAQYSAKLTDLWQAKGSRDYELDDIMLLKVGRHLRPRPHFKLIIGRELGENR
                     FLQGYRHQFTSLTAISHKGPLALIDGDAGADDIELAARILARYGQGRDAARVEVIAAE
                     PGGAETRLSVAPLAEADIGTDWHVGA"
     gene            4757..6559
                     /gene="typA"
                     /locus_tag="AC16UZ_07205"
     CDS             4757..6559
                     /gene="typA"
                     /locus_tag="AC16UZ_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR6246508.1"
                     /translation="MNTDHLRNVAIIAHVDHGKTTLVDRLLQQTGSLDRDQPERVMDS
                     NELERERGITILSKNTAIEYEGYRINIVDTPGHADFGGEVERILSMVDSVLLLVDAVE
                     GPMPQTRFVTEKAFAAGLSPIVVVNKVDRPGADPYRVIDQVFDLFDSLGASDEQLDFP
                     VVYTSALLGQAGPEPDALVDDMTPLLQSIIAHVPPPSVDADGPLQMQISSLDYSSYVG
                     VMGVGRIRRGRLARNTPVVVVDRNGRTRKARVLAVLGYHGLERIEREEAGAGDIVCVT
                     GVEEIGISDTICAPEHVEALPPLTVDEPTLTMMFCVNTSPFAGRDGSFLTSRQLRERL
                     LREASHNVALAVAETDDPDRFEVSGRGELHLSVLIETMRREGYEVAVSRPRVIMKEED
                     GRLVEPYETITIDVDDEHQGKVMESLGERRGELQEMHPDGHGRVRLRYRIPTRGLMGY
                     RPQFLSMTSGTGIMTYASAGYGPRFDGEIGRRTNGVLVSNAKGKAVAYALFNLQNRGR
                     LMIGPNDEVYEGMVVGVHSRDNDLTVNPMKEKKLTNIRASGSDENILLTTPIRLGLEQ
                     ALEFIDDDELVEITPTAIRVRKQWLKEHERKRAG"
     gene            6588..7025
                     /gene="dtd"
                     /locus_tag="AC16UZ_07210"
     CDS             6588..7025
                     /gene="dtd"
                     /locus_tag="AC16UZ_07210"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003103447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR6246509.1"
                     /translation="MRVVVQRVSSASVTVDDEAVASISTGLLVFLGVFPDDGDAQLHW
                     MADKVLGLRVFPGDDKPMNQSVVDVAGEVLVVSQFTLAADTSRGKRPGFSTAAPPDLA
                     ETIYERFVARLAALYPRVASGRFGADMKVALVNDGPVTFLLDR"
     gene            complement(7085..7864)
                     /gene="tatC"
                     /locus_tag="AC16UZ_07215"
     CDS             complement(7085..7864)
                     /gene="tatC"
                     /locus_tag="AC16UZ_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR6246510.1"
                     /translation="MAGAEDSDNRLSHDEQVDADEQPFLDHVIELRSRILRGLLVVLL
                     LFFPTYYYANDLYELVAAPLLAHLPGGGKMIATEVASPFLTPFKLSVFTAVFIGMPFL
                     LHQMWAFISPGLYLHEKKFAVPVLLSSMVLFYGGVAFAYFLVFPLVFQFMAAVTPEGV
                     TMMTDINRYLDFVLKIFFAFGFAFEIPIATFMLAWSGIATADGMANKRPYVIVGCFIV
                     GMLLTPPDVISQLLLALPTWVLFEVGIVFARLAERRSNEPG"
BASE COUNT         1404 a         2384 c         2597 g         1536 t
ORIGIN      
        1 agtccctggc aaatttgacc cggccgccga gctccgcatc caccagccgc accacgtgct
       61 cgagaagctc gtgaatgttc acgtccgcgt actcgggctg acggttcgat cccagcatgt
      121 cgtccacgag ctgggtgagg cggtccgcct cctcgatgat gatgcgcgtg tactcggtca
      181 gctcgctggc gtcgagctcg cgctggagca gctgggcagc gccccgcagg ccgccgagcg
      241 ggttcttcac ctcgtgagcg agccccctga tcagagtacg ggtagtctgc tgggagaaga
      301 acgagtgctc gtcgcggttg atccgaagca ggcggttcaa gggctgcagc tcgatgacca
      361 ggtgaccgga gttcggctcc agggaggcgg tcaggtcggc aatcacctcg gtgccgtcgc
      421 gcagctggaa ttgcgccgct cttttggtga tggactgctc tgtggtgaag atgcccgcca
      481 gatccgcgaa cggcgaagcg gcggcgtcga agagcagttc cgcgagctgc accccggtgc
      541 tctgatgctc gctcgtgttc agcagagact ccgcggcggg gttcatgtag cagaccctca
      601 gagagccatc gcagacgatc accgcgcccg ttagctggtc caggatgtcg gatttggcca
      661 gagacggcat gttgtaggca gtgttcccgt gtggctgagt catggggcgt tgacggaatg
      721 cgtgctcctg acgcaagaaa cggtccaact cctcgcgatc aggctgcaag acgtgagtaa
      781 gtatttactt cggtctcatt gcctgaataa agggccgatc agggcgggca cccccttgcg
      841 ttagcggcgc cgtgccccag gcagtatgca ccaatacggt gcaaaggtcg attgtggtgc
      901 ggccggttga cggccgccct cgcgatccgg cgtggtcgaa tgggccctgc ggtgtcgggt
      961 cagttgttga tcgggttcac cgggcgcttg ggcgcgttga gaaccgaata gcgctgcagg
     1021 tggaaggtgg acgacgggcc cgcgtagatc agctttccgg cgtcgtccac gacctctgcc
     1081 ttcagcgagt gcgtaccgcg gtccacgttg ctgagggcga aacgcccggt cgtgttggtg
     1141 ccgcccgtgg gggagccatc gagcaggagg cgtattcggt gaccaggctg caggttcggc
     1201 ggctccagct tcaccgtaac cgtcacgttt cctgcgtttt cccggatgct ctcgtcgtct
     1261 gctggggaag tgaccgtcaa tgccccgtag aactcgccag aggcttcccc ttcggcgtct
     1321 ggatcaacta tccagagctg gcggccggag ccctcggtgc ctgcttctgc gggatcgaaa
     1381 gagttggggg atgtcagggt gatcgtctcg gaaggcccat cggctctggg cggcgtgtcc
     1441 gtgaacacca cgttgccgtc ctcgtcgacg gttttgtaga tcgtcgttcc ggccatggac
     1501 tgcgtgctca gcatcagcca gggcgtggat atgatgctca gaaatgcaat tcttttcagt
     1561 gttgccagta gctgcatgtt gcccgccttg ggttggattc agccattttg gaccacgccg
     1621 aggcaaaaaa aagccccaat tcgtaaacga gttggggctt tgccgcgggc gaaacccgcg
     1681 cgagggcgtc ttacacgctg taatacatct cgaactcagc ggggtgggtc gtggaattca
     1741 ggtagtccac ttcctgctgc ttgagggcga gatagccgtc gatcatgccg tcggagaaca
     1801 cgccgccggc cttgaggaag tcgcggtcct cgtcgagagc ctgcagcgcc tgctccaaag
     1861 aagagcagac ggtcggcacg ccctccagct cttcggcggg caggtcatag agatccttgt
     1921 cgaacgagtc gccaggatgg atctcgttct ggatgccgtc gagcccggcc atgagcatgg
     1981 cggcgaaggt caggtacggg tttccggtgc tgtccgggaa gcggacttcg acccgatagg
     2041 cccgggggtt tccgccctga atgtagggaa cccggatcga tgcggatcgg ttgcgggcgg
     2101 agtaagccag gagcacgggg gcctcgaagc ctggcaccag ccgcttgtag gagttggtgc
     2161 tggcgttcgc aaacgcgttg atggcccttg catgcttgat gatcccgccg atgtagtaca
     2221 gagccgtctt tgagagcccc gcgtactcgt cgccgtggaa tacgttggtg tcccccttgg
     2281 cgatggactg gtgaacgtgc atgccgttgc cgttgtcgcc aacgagcggc ttgggcatga
     2341 aggtcgccgt cttgccgtaa gcgtccgcca cgttgtgcac gcaatatttg tatatctgca
     2401 cctcgtcggc tttacgcacc agagtgttga agcgtgcgcc gatctcgttc tgacaggcgg
     2461 ttgccacttc gtggtggtgc acctcgacgt ccaggcccat ggccatcatc gccgagcaca
     2521 tggccgagcg cagatcggcg gcggaatcca ccgggggcac ggggaagtag ccgcccttca
     2581 cggacgggcg gtggcccaga ttgccacctt cgaactcgga atgggattcc caggctgcct
     2641 cctcagcgcc gatgctgtag gacgcgccgc tcatttccac cttccagcgc acgtcgtcga
     2701 acacgaaaaa ctcgttctcg ggcccgaagt aggcggtatc gccgattccg gtggaagtca
     2761 ggtaagcctc cgcccgcttg gccagagagc gcggatcccg gtcgtagggc tgcatggtgg
     2821 agggctcgat gatgtcgcag cgcaggttca gggtcgtgtc gtcccggaac gggtcgatga
     2881 cgggagtgga gtcgtcgggc atgaggatca tgtccgactc gttgatggac ttccagccgg
     2941 agatggacga gccgtcgaac atgaaaccgt cattgagcgc gtcggcgtcg agccggtgcg
     3001 ccggtatggt ggtgtgctgt tcctttccct tcgggtcggt gaagcgcaga tccacccact
     3061 tgacgtcgtt gtccttgatg agtttcagag tattttcggc cattgtgtgt acgtgctcct
     3121 ttctagggtg tgcgtagtaa tgtcttttgc ggtatccgcg ccgtgcgacc gcggaatgca
     3181 gtaagcgtgc cacggccatt ttcttgatat cagaggttag tgccggctca caggcgctca
     3241 gaaatagtgc gaatggatgc gagaaagtgg tgcttacgca ccattttggt gcactatcag
     3301 cattactggc gcactttgat cctgactctt atttcacgtc ctccccggat caatctgtct
     3361 gcgtcggcct tcccttcggc ggccgcttct tcttcacctt ccgtcatcga gatgagctcg
     3421 tgcccgtgga cccggcacca ggccggaatg tcctgcatca cgcctgggtc ggtggcaacg
     3481 atctcgacga cggtgcccgg tgggagccct ttgacctttt cctgggttct tatcaccggc
     3541 agggggcaca gtaacccccg agcatccaat gtttcaagct ccgacatgcc aatcggtccc
     3601 aatatcggcc tcggcgagcg gcgcaacgct caggcgggtt tccgcgccgc ctggctccgc
     3661 ggcgatcacc tcaaccctgg ctgcatctct gccttgaccg tatcgggcca ggatacgtgc
     3721 tgccagctcg atgtcatcgg cgccggcgtc tccgtcgatg agggccagcg gccccttgtg
     3781 gctgatggcg gtcaggctcg tgaactggtg gcggtagccc tgcagaaacc ggttctcgcc
     3841 gagctcgcgg ccgatgatca acttgaaatg gggccgcggc cgcaggtggc ggccgacctt
     3901 cagcagcatg atgtcgtcca gctcgtagtc tcgcgatccc tttgcctgcc agagatcggt
     3961 cagcttggcc gagtactgcg cgttcgtgag gaaacagcac cctccagccg gctgggacca
     4021 gtcctcgaag ccgaagcggc gcgccagagc gaactgcggc ttgcggttgc gtccatggaa
     4081 gtcgtagagg gcttcgcgat cgacccagcc ctcgcgttca ggcagcgtcg ggggaaggtg
     4141 cttggcgcat agcggtctga gcagccggtc gtctgctccg gactcccgcg caatgatcgg
     4201 catggtttcc ttgcgctggc ttttcggacg ctgaccgatg acctccccgg tgacgatgaa
     4261 gtcaaagccg ttttcctcca tgaagctcca ggccagcgcc ttgcgcacca tgaatacttt
     4321 gcagtccagg caggggttca gatgagcccc gtagccgtgc ttcggattga gcaggacgtc
     4381 cttgtactcg tcgatgacgt cgatgatgtg cagcctgata ccgagctgct cggcaaccca
     4441 cagcgcgttg ttgcgcttgg gcttgttgcg gtccttttcc cggatggcgt gagtgtggcc
     4501 ctccacgcag aagcccgtga agaagttgac gccctccacg tggacgccct gctccatgac
     4561 gacctttgcc gccagcatgg agtcgagacc gcccgaaatt aacgcgacag cccttcgctg
     4621 ttccgccata attcgccacc tttttggccg cggcataacc gctgccgctg ctgcccgatt
     4681 gatgctcccc cgccgcaggc gccgttcatg ccgtctgtcg gctttggtgc ttcaagccgc
     4741 gcattgtaat ggatcaatga acactgacca cctgcgcaac gtcgccatca tcgcccatgt
     4801 cgaccatggc aagaccaccc ttgtagaccg gctgctgcag cagaccggct cgctcgaccg
     4861 cgaccagccc gagcgggtta tggacagcaa cgagctcgaa cgggagcgcg gcatcaccat
     4921 tctgtcgaag aacacggcca tcgagtacga aggctaccgg atcaatatcg tcgatacccc
     4981 gggtcatgcg gatttcggcg gcgaagtcga acgcatcctc tccatggtgg actcggtcct
     5041 cctgctggta gacgccgtcg aagggcccat gccccagacc cgcttcgtga cggagaaggc
     5101 gttcgccgcg gggttgagcc ccatcgtcgt ggtcaacaag gtggatcgtc ccggcgccga
     5161 tccttaccgg gtaatcgatc aggtgttcga cctgttcgac agcctcgggg cgagcgacga
     5221 gcagctcgac tttcccgtgg tctacacctc ggcgctgctg gggcaggcgg ggcccgagcc
     5281 cgacgcgctg gtagacgaca tgaccccgct gctgcagagc atcatcgccc acgttccgcc
     5341 tcccagcgtg gacgcggacg ggccgctgca gatgcagatc agctccctcg actactccag
     5401 ctacgtcggc gtgatgggcg tgggacgcat acgtcgcgga cggctcgcgc gcaacacccc
     5461 ggtggtcgtt gtggatcgca atggccggac gcgtaaagca cgggtgctgg ccgttctcgg
     5521 ctaccacggc ttggagcgga tcgagaggga ggaggcaggc gcgggcgaca tcgtgtgcgt
     5581 gacgggcgtc gaggagatcg gcatttcgga cacgatctgc gcgcccgagc acgtcgaagc
     5641 gcttcctccg ctgactgtgg acgagccgac cctgaccatg atgttctgcg tgaacacctc
     5701 ccccttcgcc gggcgcgacg ggtcgttcct cacgagccgc cagctgcgcg agcgattgct
     5761 cagggaagcc tctcacaacg ttgcgcttgc ggtggccgaa accgacgacc cggatcggtt
     5821 cgaggtctcg ggccgcggcg agctccacct ctcggtgctg atcgagacca tgcgcaggga
     5881 aggctacgag gtggcggtct cgagaccccg ggtcatcatg aaggaagagg atggtcggtt
     5941 ggtcgaaccc tacgagacga tcaccatcga cgtggacgat gagcaccagg gcaaggtgat
     6001 ggagagcctt ggtgagcgcc gcggcgagct gcaggagatg cacccggacg gccatgggcg
     6061 ggtgcggctg cgctaccgga ttcccacgcg cgggctgatg ggctatcgcc ctcagtttct
     6121 gtccatgacg tcgggcaccg gcatcatgac gtacgcctcc gccgggtacg gtccgcgctt
     6181 cgacggagag atcggccgcc gcacgaacgg ggtgctcgtc tccaacgcca agggcaaggc
     6241 ggtggcctac gcgctgttca acctgcagaa ccgcggccgg ctgatgatcg gtccgaacga
     6301 tgaggtttac gaaggcatgg tggtcggcgt gcacagccgc gacaacgacc tgacggtcaa
     6361 ccccatgaag gagaagaagc tgaccaatat tcgcgcatcg ggcagcgacg agaatattct
     6421 gctcaccacg ccgatcaggc tcgggctgga gcaggcgctc gagttcatag acgatgacga
     6481 gctggtcgag atcacgccaa ccgcgatacg cgtccgcaag cagtggctga aagaacatga
     6541 gcgcaagcgc gctggctgaa accgttcccg atcgggggcg tgtttagatg cgcgtcgtcg
     6601 ttcagcgggt ttccagcgcg tcggtgaccg tcgacgacga ggccgtcgcg tctatctcaa
     6661 cggggttgct ggtcttcctg ggggtgttcc ccgacgatgg tgacgcgcag ctccactgga
     6721 tggccgacaa ggttctcgga ctgcgcgtct ttcccggtga tgacaagccg atgaaccagt
     6781 ctgtggtcga tgtcgccggc gaggtgctcg tcgtgtccca gttcaccctc gcggcagaca
     6841 cgtcccgggg caaacgcccc ggattcagca cggccgcgcc accggacctg gcggagacca
     6901 tctatgagcg cttcgtggct cggctggcgg cgttgtaccc gcgggtagcg tccgggcggt
     6961 tcggtgcgga catgaaggtg gcgctcgtca acgacggccc ggtgaccttc cttctcgacc
     7021 gctgatccgc ggattcagac tggtcccatg ggtcgctcct ggatccggcg ggagggcgac
     7081 gatctcaacc cggctcgttg ctccggcgct cggccagccg ggcaaagacg atcccgacct
     7141 cgaagagcac ccaggtcggc agggccagca gcagctggga gatcacgtcg ggcggtgtga
     7201 gaagcatacc gactatgaag cagccgacga tcacgtatgg acgtttgtta gccatgccgt
     7261 cggcggtggc gatgcccgac caggccagca tgaaagtggc aatgggaatt tcgaacgcga
     7321 agccgaacgc gaagaatatc ttgagcacga aatccagata tcgattgatg tcggtcatca
     7381 tggtcacgcc ctccggcgtg acagcggcca tgaactgaaa gaccagggga aagaccagaa
     7441 aatacgcgaa cgcgacgccg ccgtagaaaa gcaccatgct ggacagcagc acggggacgg
     7501 cgaacttctt ctcgtgcagg tacagccccg gcgagatgaa cgcccacatc tggtgcagca
     7561 gaaacggcat gccgatgaac acggcggtga agaccgacag cttgaagggc gtgaggaatg
     7621 gcgacgccac ctcggtagcg atcatcttgc cgcctccggg caggtgcgcc aacagcggag
     7681 ctgcgaccag ctcgtacagg tcgtttgcgt agtagtaggt agggaagaag agcagcagga
     7741 ccaccagaag gccgcgcagg atgcgcgatc tgagctcgat gacgtgatcg agaaacggct
     7801 gctcgtcggc gtctacctgc tcgtcgtggc tgagccggtt gtcggaatct tcagcgcccg
     7861 ccattgtccg cagtggccga ggtttcgttt gaaggcgggt ttccggcagc ctcggtctga
     7921 g
//
LOCUS       DBNHOX010000330         2213 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1504859, whole genome shotgun sequence.
ACCESSION   DBNHOX010000330 DBNHOX010000000
VERSION     DBNHOX010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1504859"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="AC16UZ_07220"
     CDS             <1..1488
                     /locus_tag="AC16UZ_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6246511.1"
                     /translation="KGLDLQFHGAANLPVALVGDPRRLGQVLAVFIGNAIKFTDRGSI
                     RVEVGLASATDDTASLEFSVTDSGPGMSAEEQKHLFDPFSQADESHTRTHGGTGLGLA
                     VASKLAALMDARIDVSSEPGRGSTFSLVLTLPLATDDAGDGPDSGTDTVLDLGPIRGA
                     RVLLVDDSEINLQVASEILRQIPVRVEVAHDGQEAVTMTESHHYDCVLMDVQMPVMDG
                     YAATEAIRAGPLGEDLPILAMTANVAPEDQARAKAAGMNAHIAKPIDPDRLFRELLTW
                     IEPGEREPIEPELAVEPASLAELPDELPGIRVGEGLARVGGNVDLYVSLLKDLARDYA
                     DCHTQLGELLGAGATQEAAALAHKLKGIANNLGVVGVGTQAAALESALRSAEPVSESA
                     LQNLAEALAESSTAIEQLVALQSAAEGGGEESGGNSLELLDRLEKEIGENDPAASDTA
                     EELLKSLEADSAAVTAATAARDALELFDFAGAGEQLDQIRAGLGS"
     gene            complement(1540..>2213)
                     /locus_tag="AC16UZ_07225"
     CDS             complement(1540..>2213)
                     /locus_tag="AC16UZ_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR6246512.1"
                     /translation="IGAGQLVVDYGCGPGGLAVELARRVGSTGKVIGLDINAEFLERA
                     RARAADEGVAERVETRLVTGDRFPMDTHAVDRVICKNVLEYVPDPQATINEFHRILRP
                     GGIAHVSDSDWGAVVFEPAREPFARIMSAAAVAFRTPLIGRSLYGMFRRAGFDDVRVQ
                     VLAAADTTGALRGVLHNMAGYALTSGQVDRADVDAFISDIDRALDDKTYLAVLPQFLV
                     TGRVP"
BASE COUNT          381 a          709 c          753 g          370 t
ORIGIN      
        1 aaggggctcg accttcagtt tcatggggcg gcgaaccttc ccgttgcgct ggtgggcgac
       61 ccccggcggc tgggccaggt gctggccgtc ttcatcggca atgccatcaa gtttacggat
      121 cggggcagca tccgtgttga ggtcggcctt gcgtccgcaa ccgacgacac cgccagcctt
      181 gaattcagcg tcacggactc ggggccgggc atgagcgccg aggagcaaaa gcatctgttc
      241 gatccgtttt cccaggcgga cgagagccat acgcggaccc acggaggcac agggttaggt
      301 cttgccgtcg cgtcgaagct agcggcgctc atggatgcgc gcatcgacgt cagcagcgaa
      361 ccgggccggg gcagcacgtt ttcgcttgtg ctgacgctgc ccctggcaac tgacgatgct
      421 ggtgacgggc cggattcggg taccgacacg gtgctcgatc tgggcccgat tcggggcgcc
      481 cgggtgctcc tcgtggatga ctcggagatc aaccttcagg tggcctcgga gattcttcgc
      541 cagattccgg ttcgggtgga ggtggcccac gacggccagg aagccgtgac catgaccgag
      601 agccaccatt acgactgcgt gctgatggac gtgcagatgc cggttatgga cggttacgcc
      661 gccacggaag cgatccgtgc cgggccgctc ggcgaggatc tgccgatact ggccatgacg
      721 gccaacgtcg cacccgagga ccaggccagg gcgaaagccg ccgggatgaa cgcgcacatc
      781 gcgaaaccca tcgatcccga caggcttttc cgggagctgc tgacctggat cgagccgggg
      841 gaaagggaac ccattgagcc ggagttggcc gtcgagcccg cctccctcgc tgagcttccg
      901 gacgagttgc ccggcattcg ggtcggcgaa ggccttgccc gcgtcggagg gaacgtcgat
      961 ctgtacgtct cgctgttgaa agatctcgcc agagactatg ccgactgcca cacgcagctc
     1021 ggcgagctgc ttggcgctgg cgcaacgcag gaagcggccg cgctcgccca caagctcaag
     1081 ggcatcgcca acaacctcgg tgtggtaggc gtcgggaccc aggcagcggc gttggagtct
     1141 gccctgcgat ccgccgagcc cgtatcggaa tcagcgctgc agaatctggc tgaagcgctg
     1201 gcagaatcct ccacggccat cgagcaactc gtggcattgc agtccgcggc ggaaggcggt
     1261 ggcgaagagt ctggcgggaa ttcgctggag ctcctggacc gcctcgaaaa ggaaattggc
     1321 gagaacgatc cggcagcctc tgacaccgcc gaggaactcc tcaagagcct cgaggcggac
     1381 tctgccgcgg tcacggcggc caccgccgct cgggatgccc tggagctgtt cgactttgcc
     1441 ggtgccggcg agcagctgga ccagattcgc gcgggtcttg gatcctgaat tcttagcggc
     1501 ctcaccggct gtgctggcag cccggccact cgccgccggt tacggaacgc gaccggttac
     1561 gagaaactgg ggcaatacgg ccaggtaggt tttgtcgtcc agcgcgcggt cgatgtcgga
     1621 gatgaacgca tcgacgtccg ctctgtcgac ctggccggat gtgagcgcgt aaccggccat
     1681 gttgtgcaga acgccgcgca gggcgccggt cgtgtcggcg gcggcgagga cctgaacgcg
     1741 cacatcgtcg aatccggcgc ggcggaacat gccgtacagg ctgcggccga tcaggggtgt
     1801 ccgaaaagcc accgcggctg cggacatgat ccgggcgaac ggctcgcggg ccggttcgaa
     1861 gaccactgca ccccagtcgc tgtcgctgac gtgggcaatg ccgccgggcc gcagaattcg
     1921 gtggaactcg ttgatggtgg cctgtggatc cggcacgtat tcgagcacgt tcttgcagat
     1981 cacccgatcg accgcgtgcg tgtccatggg gaacctgtcg cccgtgacga gccgagtctc
     2041 gacgcgctct gctaccccct cgtctgccgc ccgggcccgg gcgcgctcga ggaattcggc
     2101 attgatgtcg agcccgatga ccttgcccgt gcttcccacc cgacgcgcca gctctacggc
     2161 tagccccccg ggaccacatc cgtagtccac gacgagctgg cccgcgccga tag
//
LOCUS       DBNHOX010000331         5931 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_797321, whole genome shotgun sequence.
ACCESSION   DBNHOX010000331 DBNHOX010000000
VERSION     DBNHOX010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_797321"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1315
                     /locus_tag="AC16UZ_07230"
     CDS             101..1315
                     /locus_tag="AC16UZ_07230"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6246513.1"
                     /translation="MSFDVDRLRLDFPILGREVNGAPLVYLDNAATSQKPEAVLHAID
                     AYYRTCNANVHRAAHALSDEATRAFEDARERVRQFINATSTSEIVFTRGTTEAINLVA
                     SCFTQRLTAGDEILISHLEHHSNIVPWQMLAQRTGARLVAAEITESGDIDLEDFRAKL
                     SERTQMVAIGHVSNALGTINPVRQIVELARSAGAATLIDGAQAVAHLPVDVRALDCDF
                     YAFSGHKMFGPTGTGVLFGRESLLDAMPPWQGGGEMIEHVSLEESTYNRLPYKFEAGT
                     PNIAGAIGLSAAVDYLSNVPRDDARLHEDHLVDLTLSQLRQMDGVRLIGEPAERSSVV
                     SFLVDNSHPHDIGTLLDQQGVAVRTGHHCAMPLMQRLGIPGTIRASLGLYNSEADVER
                     FVAALRKALSFV"
     gene            1347..1703
                     /locus_tag="AC16UZ_07235"
     CDS             1347..1703
                     /locus_tag="AC16UZ_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR6246514.1"
                     /translation="MSVRTFEPTQIAVTPAAAAHIRSQIATSGHKYLRLGVKESGCNG
                     YMYTLDYLDEPAENDRAFEVADSLSLYVSTDDLPMVSGTEVDYVTEGLNSSLKFKNPN
                     AESHCGCGESFSIAGS"
     gene            1718..2242
                     /gene="sufT"
                     /locus_tag="AC16UZ_07240"
     CDS             1718..2242
                     /gene="sufT"
                     /locus_tag="AC16UZ_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HIR6246515.1"
                     /translation="MEKRIIATTRECPARLVPTGDPITIPSNTFVTLTQNLGGTYTVI
                     MNGNMARVDSADADALGFEPQTLEFEPPADGKVDMEQVKAALATVYDPEIPVNVVDLG
                     LIYDCRLTDRHVHIAMTLTAPGCGMGPVLVEDIKSRVGRVPYVESVDVELVFDPPWSR
                     DMMTAEAQLELGLF"
     gene            2246..2662
                     /locus_tag="AC16UZ_07245"
     CDS             2246..2662
                     /locus_tag="AC16UZ_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HIR6246516.1"
                     /translation="MDIGDIRDSFAFFDSWEDKYRFVIEIGRDLPPLDAALKTEDNLV
                     RGCQSQVWLVAAYDPVADRLTFAIDSDAHIVRGLIAIILAAFDKRSPAEILAFDVEAL
                     FEELELLQHLSPSRGNGLRAMVGRIRGIAAENAAGA"
     gene            complement(2694..3308)
                     /locus_tag="AC16UZ_07250"
     CDS             complement(2694..3308)
                     /locus_tag="AC16UZ_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR6246517.1"
                     /translation="MSHLGTLCIFARAPERGAVKTRLAARIGADAALAAYQRLVDLAL
                     SRYAALPDLRSELWVSGDRCLPEVRGWSADWGLPLFEQRGGDLGERMAAAMAHGCARD
                     VATLIVGIDCPCIDGAYVRAAAQTLLTTDLVLAPAEDGGYGLIGLRRPMPELFRGVAW
                     GSSSVLAETLVKAERLRLRYELLETVWDVDDAADWERFLESGYG"
     gene            complement(3305..3985)
                     /locus_tag="AC16UZ_07255"
     CDS             complement(3305..3985)
                     /locus_tag="AC16UZ_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR6246518.1"
                     /translation="MRSISVLIPVLNDAAALRRLLDRLEALVSESIIGCEIVVIDGGS
                     TDGSQNVCAERDCLLIQSAGGRGRQLQAGFEASSGDLIWMLHADTDPDSEALKYLVTL
                     DAPAWGRFDVRFADGPPFLSVVATLMNWRSRMTGIATGDQAIFAHRSLLEAIGGVPRQ
                     ALMEDIELSKRLKTRMRPLCSRHIVETSGRRWEARGVIRTILSMWRFRLRYWIGADPE
                     RLAAEYYS"
     gene            complement(3993..5414)
                     /locus_tag="AC16UZ_07260"
     CDS             complement(3993..5414)
                     /locus_tag="AC16UZ_07260"
                     /inference="COORDINATES: protein motif:HMM:NF005687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR6246519.1"
                     /translation="MSGELWRLSAVELAEGIRQGEYSSRDVVASVSARIADRNPELNA
                     IVYDCRDEALAAAERADATLAEGSEVGPLHGVPVTIKENVDQEGKPTPNGVRAFEQII
                     APADAPLVTNLRNAGAIVIGRTNTPELSMRATTDNPLHGRTYNPWDPEASAGGSSGGA
                     GAAAAAGFGPIHHGNDIGGSLRFPSFCNGVTSVKPTFGRIPSYNPSMTGERGMLAQLM
                     SVQGAICREVRDVRLATRVMAHGDPRDPWWVPAPFEGEPAPRRVALTKENYGYPIHPE
                     LLSALDRAAAHLVDAGYQVEEVTTPSIAEPAQGWFDVAIYEMKETIGPVVEQYGSETL
                     KQIFHWYYDFGDLVDADGYREAIAARTTLTRRWNLFLDEYPLVLSPFLMRPTYPWNYD
                     AVSYEQAHDLFNSGIYSFGINYLGLPAGVVPVGLVEGLPAGVQIVGRRFREDLILDAM
                     EAVEARVGVLAHELWRSEAPAAS"
     gene            complement(5556..5855)
                     /locus_tag="AC16UZ_07265"
     CDS             complement(5556..5855)
                     /locus_tag="AC16UZ_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246520.1"
                     /translation="MRKILLPLALMLTGTAWAECACFCADGALTTMCTTVAEAKANAG
                     MCAARDPGSCPVGPLQTERVRYASPEEGAVNCRGARVWDAARGTFADVRVCDVAP"
BASE COUNT         1130 a         2007 c         1812 g          982 t
ORIGIN      
        1 aggcccaggc acggctgctg ctgtgcaacg gattcctccg ggagtgcatc aaggggccat
       61 tcgctgatgc cgtcatggcg cggatccggg aagccctcca atgagcttcg acgtcgaccg
      121 actgcggctg gactttccca tcctcggacg cgaggtcaac ggggcacctt tggtgtatct
      181 cgacaatgcc gcgacttccc agaagccgga agccgtgctc catgccatcg acgcctacta
      241 tcggacctgc aacgcaaacg tccaccgagc cgcgcacgcg ctctccgacg aggcaacccg
      301 agcgttcgag gatgcacggg aacgcgtgcg tcagttcatc aacgcgacgt cgaccagcga
      361 gatcgtcttc acacggggca ccaccgaggc catcaacctc gtggccagct gcttcaccca
      421 acgcttgacc gccggcgatg agattctgat ctctcacctc gagcaccact ccaacatcgt
      481 cccctggcag atgctcgcgc agcggacggg cgcgcgcctc gtggccgcgg agatcaccga
      541 atccggagac atcgacctcg aggactttcg ggcaaaactc agcgaacgta cgcagatggt
      601 cgccatagga cacgtctcca acgcgctcgg gacaattaac cccgtcaggc agatcgtcga
      661 gcttgccagg agcgcgggcg ccgcaacgct gatcgacggc gcccaggcag tcgcccacct
      721 gccggtggac gtccgcgcgc tggattgcga cttttacgcg ttctcaggcc acaagatgtt
      781 cggccccacc ggcaccggcg tgctcttcgg acgcgaatcg ctgctcgatg cgatgccccc
      841 atggcaggga ggcggcgaga tgatcgagca cgtctccctc gaagagagca cctacaaccg
      901 gctgccctac aagttcgaag caggcacccc caacatcgcc ggcgccatcg gcctctcggc
      961 cgccgtggac tacctgagca acgtgccacg ggatgacgcc cgccttcacg aggatcacct
     1021 ggtcgatctg acgcttagcc agctgcggca gatggacggc gtccggctca tcggcgagcc
     1081 cgccgaacga tcgtcggtgg tgtccttcct ggtggacaac agccatccgc acgacatcgg
     1141 cacgctgctc gaccagcagg gtgtcgccgt gcgtacgggt caccattgcg ccatgccgtt
     1201 gatgcagcgt ctggggattc ccggcaccat ccgagcttct ctgggtctgt ataattcgga
     1261 agccgacgtg gaacgattcg ttgccgcgtt gcgcaaagct ctgtctttcg tgtgacccaa
     1321 gcaagaggag gagcaagagg agaatcatga gcgttcgcac cttcgaaccc acccagatcg
     1381 cggtgacgcc cgccgcggct gcccacatcc ggagccagat cgccacgtcc ggccacaaat
     1441 atctgcggct gggcgtcaag gaaagcggct gcaacggcta catgtacact ctggattacc
     1501 tggacgagcc tgcagagaac gaccgggcgt tcgaggttgc cgattctctg agcctctacg
     1561 tgagcaccga cgacctgccc atggtgagcg gcacggaggt cgattacgtg accgagggcc
     1621 tgaactcgag cctcaagttc aagaacccca acgccgagtc tcactgcggt tgcggggaaa
     1681 gcttctccat cgcaggttcc tagagagcga ccggccggtg gaaaagcgca tcatcgccac
     1741 caccagggag tgcccggcaa ggctcgtccc gacaggtgat cccatcacga tcccatccaa
     1801 cacctttgtg acgttgaccc agaatctcgg cgggacgtac accgtgatca tgaacggcaa
     1861 catggctcgt gtggacagtg ccgatgctga cgctttgggg ttcgagcccc agaccctcga
     1921 attcgagcct cctgcggatg gcaaggtcga catggagcag gtcaaggccg ccttggcaac
     1981 cgtttacgat ccggagatcc ccgtcaacgt cgtcgatctc gggctgattt acgattgcag
     2041 gctgaccgac cggcacgttc acatcgccat gaccctcacc gcaccgggtt gcgggatggg
     2101 acccgtgctg gtcgaggaca tcaaatccag ggtcggacgg gttccctacg tcgaatccgt
     2161 ggacgtggag ctcgtcttcg atccgccctg gtcccgggac atgatgaccg cggaagccca
     2221 gctggagctc ggcctgttct gatccatgga catcggcgac atccgcgaca gctttgcgtt
     2281 tttcgattcc tgggaggaca aataccgctt cgtcatcgag atcgggcgtg accttccacc
     2341 gctggacgcc gccctgaaga ccgaggacaa cctggtgcgc ggctgccaga gccaggtgtg
     2401 gctggtagcc gcctacgacc cggttgccga caggctcacc ttcgccatcg acagtgacgc
     2461 gcacatcgtc cggggactga tcgcgatcat ccttgctgct ttcgacaaac gctcgcccgc
     2521 cgagattctg gcattcgacg tggaagccct cttcgaggag ctggagctgc tccagcatct
     2581 cagccccagc cggggaaacg ggttgcgtgc gatggtcggc aggatcagag gcatcgctgc
     2641 cgagaacgcg gcaggcgcct gatcgaagca cgcatcgccc ggctccccaa gattcacccg
     2701 tagccggact cgagaaatcg ctcccagtcc gccgcgtcat ccacgtccca gacggtttcg
     2761 agcagctcgt atcgaagccg cagcctctcg gctttcacca gcgtttcggc gaggacgctg
     2821 ctgctgcccc acgccacgcc ccggaacagc tcgggcattg gccgccgcag cccgatcagc
     2881 ccgtaaccgc cgtcctccgc gggtgccaga acgagatccg tcgtgagaag cgtttgggcc
     2941 gcagcgcgca cataggcgcc gtcaatgcat ggacagtcga ttccaacgat cagcgttgca
     3001 acgtcccggg cacagccgtg ggccatggct gcggccatgc gctctcccag atcgccacca
     3061 cgctgctcga acaacggcag gccccagtcc gccgaccacc cgcgcacttc cggcaagcaa
     3121 cgatcgccgc ttacccagag ctcggagcgg agatcgggga gtgcagcgta gcgggagagt
     3181 gcaaggtcaa cgaggcgctg ataggcggcc aacgcagcat cggcaccgat tcgcgcagcc
     3241 aggcgcgtct tcaccgcgcc tcgctccggc gcgcgcgcga agatgcacaa cgttcccaga
     3301 tggctcacga gtagtactcg gcggccaggc gctcgggatc ggctccgatc cagtagcgca
     3361 gacgaaaccg ccacatgctc aatatggtgc ggatcacgcc gcgggcttcc cagcgacgcc
     3421 ccgaggtctc cacgatatgg cgcgagcaga gcgggcgcat acgggttttg agacgtttcg
     3481 acagctcgat gtcctccatc agcgcctggc gtggcactcc gccaatcgct tcgagcaacg
     3541 agcggtgggc gaagatggcc tgatccccag tggctatacc ggtcatccgg gagcgccagt
     3601 tcatcaacgt cgccaccact gagagaaagg gcggcccgtc cgcaaaccgg acgtcaaagc
     3661 gcccccacgc cggagcatcg agggtaacaa ggtatttaag cgcttcagaa tccggatcag
     3721 tgtcggcatg cagcatccag atcagatcgc cggagctcgc ctcgaaaccc gcttgcagtt
     3781 gccggcccct gccgcccgcg gattgaatga ggaggcaatc gcgctcggcg cagacgttct
     3841 gcgagccgtc ggtgctgccg ccatcgatca cgacgatctc gcacccgatg atcgactccg
     3901 aaaccagggc ctccaggcgg tcgagcaatc ggcgcaaggc ggctgcgtcg ttcaggacgg
     3961 gtatcagaac gctgatgctt cgcatccggg tatcagctgg cggctggggc ctcggaccgc
     4021 cacagctcgt gggccagcac gccaacccgg gcctccacgg cctccatggc atcgagaatc
     4081 agatcttccc ggaaacgccg accaacgatc tgcacccccg ccggcagacc ttccaccaga
     4141 ccaacgggaa caacccccgc ggggagcccc agatagttga tgccgaaact gtagatgccc
     4201 gagttgaaaa gatcgtgcgc ctgctcatag ctgaccgcat cgtagttcca cggataggtt
     4261 ggccgcatga gaaacgggct gagaacgagc gggtactcgt ccagaaacag gttccagcgc
     4321 cgcgtcaacg tggtgcgagc ggcgatcgcc tcgcgatagc cgtccgcgtc caccagatcg
     4381 ccgaagtcgt agtaccagtg gaagatctgc ttgagcgttt cgctgccata ctgctccacc
     4441 accgggccga tggtttcctt catctcgtat atcgcgacgt cgaaccagcc ctgggcgggt
     4501 tccgcaatgg acggcgttgt cacctcctcc acctggtagc cggcatcgac gaggtgagcc
     4561 gccgcccgat ccagagccga gagcagctcc gggtgaatcg gatagccgta attctccttg
     4621 gtgagcgcga cgcggcgagg tgccggctca ccctcaaacg gggcgggcac ccaccacgga
     4681 tctctgggat ctccatgggc catgactcgc gtggccaggc gcacgtcgcg aacctcccgg
     4741 caaatcgccc cctgcacgga catcagctgt gccagcattc cccgttcccc cgtcatcgag
     4801 gggttatagc tcggaatcct gccaaatgtc ggtttgacgc tggtgacgcc gttgcagaat
     4861 gacggaaagc gcagggagcc gccgatgtcg ttgccgtgat ggatggggcc gaaaccggcc
     4921 gctgccgcgg cgcctgcacc acccgatgag cctcccgcgc tggcttccgg atcccaggga
     4981 ttgtacgtcc gcccgtgcag cgggttgtcc gtggtcgccc gcatggagag ctcgggcgtg
     5041 ttcgtgcgcc cgatgacgat ggcccccgcg ttacgcaggt tggtgacgag gggagcgtcg
     5101 gcgggtgcga tgatctgctc gaaggcgcgg accccgttag gcgtcggctt gccctcctgg
     5161 tccacgttct ccttgatggt caccggcaca ccgtgaagcg gccccacctc ggagccttca
     5221 gccagcgtcg catcggcccg ctcggcggcc gccagcgctt cgtcgcggca gtcatagacg
     5281 atggcgttca attccgggtt gcgatcggcg atccgcgcag aaacgctcgc caccacatcc
     5341 cggctggaat actcaccctg gcggattccc tccgccagct cgacggcact caatcgccac
     5401 agctcaccgc tcattaccgc gccctcccgt gaataaagcg catccgcgtg aaggacaccc
     5461 accgccgcgg ccgctgaacc actataccgg caagccggac cagccgaaaa cggcgccaat
     5521 tcagcgcgag ggaggcaggg cccggtaagg ctcggctacg gcgctacgtc gcagacccgt
     5581 acgtccgcga aggtgccccg cgcagcatcc cagactcgcg cgccgcgaca gttgacggct
     5641 ccttcctcgg gggacgcgta acggacccgt tccgtctgca gcgggccgac ggggcagctt
     5701 cccggatccc gcgccgcgca cattccagca ttggccttgg cctccgccac ggtggtgcac
     5761 atggtggtga gggcgccatc ggcacagaaa caggcacact cggcccaggc ggtcccggtc
     5821 agcatcaatg caagcggcag cagaatcttt ctcatgttcg atttcctgaa gtgggggcct
     5881 caaccatagg ctaggccgat cgaacgcgat gccagcggaa gtcgccaatg a
//
LOCUS       DBNHOX010000332         1954 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1246897, whole genome shotgun sequence.
ACCESSION   DBNHOX010000332 DBNHOX010000000
VERSION     DBNHOX010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1246897"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="AC16UZ_07270"
     CDS             complement(<1..342)
                     /locus_tag="AC16UZ_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246521.1"
                     /translation="MSQERRRVVICGAAGRDFHNFNVAFRDDPQVQVVAFTAAQIPGI
                     DRRRYPAELAGSRYPEGIPIIPEAELESVCRQQSVSEVIFAYSDVSYAAVMEVASRVL
                     ATGAGFELLGPD"
     gene            complement(344..1138)
                     /locus_tag="AC16UZ_07275"
     CDS             complement(344..1138)
                     /locus_tag="AC16UZ_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246522.1"
                     /translation="MTERVTRVLLTLECDRLDADAMKAVVRLAGDGELEMLGLFVEDE
                     DLLRAARLPGLTEVSVNTGEVKTVSVEQIQEQVAGQARRAREVFEASARGLKVKHSFR
                     IARGRAVDAVAEAALTSDIVVVSRALRASGLRSRRGAHFESLVKKHGNILFVNEPWAS
                     GASVVVLCESTPTDCERPLNVARRIADAENIELVLAVPPGAQSVERSPADRVVHLDAW
                     SEDAVAALCDQEDARLLVLPPTEKLDWRALLMNIVDRVPCSLLRLD"
     gene            complement(1131..>1954)
                     /locus_tag="AC16UZ_07280"
     CDS             complement(1131..>1954)
                     /locus_tag="AC16UZ_07280"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HIR6246523.1"
                     /translation="YRLDRFRELVRENVARGVILVESSGSRVGQINGLSVVNLGQLSF
                     GQPMRITATARLGRGELIDIERESKLGGNIHSKAVMIVSSFLGSRYAREMPLSLHASL
                     VFEQSYGGVEGDSASIAEVCALISAISERPIRQCLAITGSMDQHGVAQAIGGVNEKIE
                     GFFDICVEQGLSGDQGVIIPVSNCDHLMLREDVVDAVREGKFHVYSMRTVDDAMQRLF
                     AADGETLADVETIDAAVRDRLTQWHRIWRRAAAEAGAAEGAEGGERGMGKDAGDD"
BASE COUNT          348 a          704 c          593 g          309 t
ORIGIN      
        1 gtctggcccg agaagttcga acccggcacc cgtggccaga acccgcgagg ccacttccat
       61 gaccgcggcg tagctcacgt cgctgtaggc aaagatcacc tcgctcaccg attgctgccg
      121 gcagacggac tcgagctcag cctccggaat gatgggtatg ccctcgggat accgcgaacc
      181 cgcgagctcg gccgggtagc ggcgcctgtc gatgcccgga atctgggcag ccgtgaaggc
      241 cacgacctgc acctggggat catcgcgaaa cgcgacgttg aagttgtgaa aatcacgccc
      301 ggcggctccg cagatgacca cccggcggcg ctcctggctc acttcagtcg agcctcaaca
      361 gcgagcaggg cacccggtcc acgatattca tgagcaatgc ccgccaatcg agtttctcgg
      421 tgggcgggag gaccagcagc ctggcatcct cctggtcgca gagcgcggcg acggcatcct
      481 ccgaccaggc gtccagatga acgacccgat cggccggtga tcgctccacg ctctgggcgc
      541 cgggcgggac ggcaagcacg agttctatgt tctcggcatc cgcaatccga cgggctacgt
      601 tcagcggccg ctcgcaatcg gtcggcgtgg actcgcagag cacgaccacg cttgcgccgg
      661 acgcccaagg ctcgttgacg aacaggatgt tgccatgctt tttaaccagc gattcgaaat
      721 gagcgccgcg ccgcgagcgc agtccagacg cccgcagtgc ccggctcacg acgacgatgt
      781 cggaggtcaa tgctgcctcc gccacggcgt ccacggcccg cccgcgggcg atgcggaacg
      841 agtgcttgac cttgagcccc cgggcagaag cctcgaagac ctcccgggcc cgccgggcct
      901 ggccggccac ctgttcctgt atctgctcga cggacacggt ctttacctcg cccgtgttca
      961 ccgacacctc ggtgagcccc ggcagccggg cagcgcgcag gaggtcctcg tcctccacga
     1021 acagcccgag catctcgagc tcgccgtctc cggccagccg caccaccgcc ttcatcgcgt
     1081 cggcgtccag acggtcgcat tccagggtga gcagcaccct ggtaacccgc tcagtcatcg
     1141 ccggcatcct tccccatgcc cctctccccg ccttccgcgc cttccgccgc gccggcttcc
     1201 gcagcggcgc gacgccagat ccgatgccat tgcgtgaggc ggtcccgcac ggcggcatcg
     1261 atggtttcga cgtcggcgag cgtctcgccg tccgcggcaa acagcctctg catggcatcg
     1321 tctacagtgc gcatggagta aacgtggaac ttcccttcac gaacggcatc caccacgtcc
     1381 tcccgcagca tcaggtggtc acagttcgac acggggatga tgaccccctg gtcaccgctc
     1441 aagccctgct cgacgcagat gtcgaaaaat ccctcgatct tctcgttgac gccgccgatc
     1501 gcctgggcga cgccatgctg gtccatggac cccgtgatcg ccaggcactg cctgatcggg
     1561 cgctcgctga tcgcggagat cagcgcgcag acctcggcaa tggacgcgct gtcgccttcc
     1621 accccgccgt aagactgctc gaacaccagg ctggcgtgca gagacagcgg catctcacgc
     1681 gcgtaccggg agccgagaaa ggacgagacg atcataaccg ccttggaatg gatgttgccc
     1741 ccaagcttgg attcacgctc gatgtcgatc agctcgcccc ggccaagccg ggcggtcgcc
     1801 gtgatccgca tgggctggcc gaagctcagc tgaccgagat tgaccaccga caggccgttg
     1861 atctggccga cgcgagaacc ggacgactcg accaggatca ccccgcgcgc cacgttctcc
     1921 cgcaccagct cgcgaaaccg gtcgaggcgg taga
//
LOCUS       DBNHOX010000333         2760 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_861345, whole genome shotgun sequence.
ACCESSION   DBNHOX010000333 DBNHOX010000000
VERSION     DBNHOX010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_861345"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="AC16UZ_07285"
     CDS             <1..1283
                     /locus_tag="AC16UZ_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003379714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIR6246524.1"
                     /translation="LTAVDSAIMVIDGSKGVEPRTIKLMEVCRLRDTPILTFINKLDR
                     EIRDPVEVMDEIEDILQIQCAPMTWPIGMGRQFKGIYDLYADEIVCFVQGHGHHIFDY
                     PRIKGLESNAARELLGSDHDDVVEEIELVRGASHAFDVDDYLGATLSPVYFGTALGNF
                     GVKEMLDGFVTYAPPPQARHADARDVKPGEEQFSGFVFKIQANMDPKHRDRIAFLRVC
                     SGRYAQGMRMRHVRLGKDVKVADAVTFLAGDRAQTVEAFPGDIIGLHNHGTIQIGDSF
                     TQGEVIKFTGIPNFAPELFRRVRVKDPLRSKQLERGLDELSEEGASQVFKPLIRNELI
                     VGAVGMLQFDVVAFRLREEYGADCVYDSTNIFAARWVVSDDKKKLEDFTRKHRDQLAV
                     DGGGHLAYLAPTRANLQLVEERWPEIRFLKTREH"
     gene            1284..2075
                     /locus_tag="AC16UZ_07290"
     CDS             1284..2075
                     /locus_tag="AC16UZ_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024762.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HIR6246525.1"
                     /translation="MAGLAEQELIQAVRAFASDPEADELEAAVLVNRALEPSLDADTL
                     RGRIAELAAACPETQTPWDYLRELGFVGNAANYDALDNSRLDRLLDTRRGIPIALGVL
                     LIEVARASGRMAVGVNFPGHFLVRVGESLVDPFRMTQTSEDECVAGLGQARGNPGAGG
                     DLFPSATAAAVLLRMLNNIKYRYAQAGEWHRSLDMVDLQLAAAGESAHLLLERGELWL
                     RLGARGPARADLERALALAGDGDPRLAAAARRLLDGLGSGPELLH"
     gene            complement(2093..>2760)
                     /locus_tag="AC16UZ_07295"
     CDS             complement(2093..>2760)
                     /locus_tag="AC16UZ_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HIR6246526.1"
                     /translation="SLSRKLLGDESLQHRTYMLAHGTGTPQNRVTESHILNELAKVFG
                     IERWPVAAIKAYVGHSMAPAGGDQLAAALGLWQHGWLPGITTIDHIADDVHDSNLNLP
                     LEHLELGAENVDAAFVNSKGFGGNNATGLFLSPAKTREMLRKRWGKKALAEHGRSNES
                     VEQAALDYDEAMVRGEAKPIYQFGEGVVHGEDLTISRDSIHIPGFGMPVDLDLENPFP
                     DMT"
BASE COUNT          466 a          852 c          936 g          506 t
ORIGIN      
        1 cgctcaccgc ggtcgattcc gccatcatgg tcatcgacgg ctccaagggc gtcgagccgc
       61 gcacgatcaa gctcatggaa gtctgccggc tgcgcgatac gccgattctc acgttcatca
      121 acaaactcga ccgggagatc cgggacccgg tggaggtgat ggacgagata gaggacatcc
      181 tgcagattca gtgtgcgccc atgacctggc cgatcggcat gggccgccag ttcaagggca
      241 tctacgatct gtacgccgat gagatcgtct gcttcgttca ggggcacggc catcacattt
      301 tcgactaccc gcggatcaag ggcctggaga gcaacgccgc gcgcgagctg ctgggttcgg
      361 accacgatga tgtggtcgaa gagatcgagc tggtacgcgg ggccagccac gcgttcgacg
      421 tcgacgacta tctcggcgcg acgctgtcgc ccgtctactt cgggaccgcg ctcggcaact
      481 tcggagtcaa agagatgctc gacgggttcg tcacgtacgc cccgcccccc caggcccgac
      541 acgccgacgc ccgcgacgtg aagccggggg aggaacagtt cagcgggttc gtgttcaaga
      601 tccaggcgaa catggacccg aagcaccgcg atcgcatcgc cttcctccgg gtgtgctcgg
      661 gccgttatgc ccagggcatg cggatgcgcc acgtgcggct cggaaaggac gtcaaggttg
      721 ccgatgcggt gacctttctc gccggtgacc gggcccagac ggtggaggcg tttcctggcg
      781 acatcatcgg gcttcacaat cacggcacca ttcagatcgg cgattccttc acccagggtg
      841 aggtgatcaa gttcaccggg atcccgaact ttgcgcccga gctgttccga cgggtgcggg
      901 taaaggatcc gctcaggagc aaacagctgg agcgggggct ggacgagctg tcggaggagg
      961 gagccagtca ggttttcaag cccttgatcc gcaacgagct catcgttggt gcggtgggca
     1021 tgctgcagtt cgacgtggtg gcgttccgtc tgcgcgaaga gtacggggcc gactgtgtct
     1081 acgattcgac caacatcttt gctgcgcgct gggtggtctc ggacgacaag aaaaagctcg
     1141 aagacttcac gcgcaagcac cgcgatcagc tggccgtgga tggcggagga caccttgctt
     1201 accttgctcc gacccgggcg aacctgcagc tggtggagga gcgctggccc gagatccgct
     1261 ttctcaagac ccgggagcat tgattggccg gcctcgcaga gcaggaactg attcaagcgg
     1321 tcagagcgtt tgcctcggac cctgaagccg acgagctcga agcggccgtg ctcgtcaatc
     1381 gcgctctgga gccgtctctg gatgcggaca ccctgcgtgg gcgcattgcc gagcttgcag
     1441 cggcctgtcc ggaaacgcag acaccctggg attacctccg ggagcttgga ttcgtcggca
     1501 atgccgcgaa ttacgacgcg ctcgacaaca gccggctgga ccgtctgctc gacacccggc
     1561 ggggcatacc catcgccctg ggcgtcctgc tgatcgaggt ggcgcgcgcc agcggccgca
     1621 tggccgtcgg cgtcaacttt cccgggcact ttctggttcg ggtgggagaa agtctggtag
     1681 atccgttccg gatgacgcag acctccgagg acgaatgcgt tgccggtctc ggccaggcgc
     1741 gagggaaccc gggcgcgggc ggcgatctct ttccttcggc caccgcggcg gccgtcctgc
     1801 tgcgtatgct caacaacatc aagtaccgct acgcccaagc gggggagtgg catcgcagcc
     1861 tcgacatggt ggatctgcag ctagcggccg cgggggagag cgcgcatctg cttctcgaac
     1921 gcggtgagct gtggttgcgg ctaggcgcca ggggaccggc acgtgccgat ctggagcgcg
     1981 cgttggctct tgctggggac ggcgatcccc gcttggccgc cgcggctcga cggttgctcg
     2041 acgggctggg gtccggcccg gagctgctgc actgattgcg gggctcagcg cctcaggtca
     2101 tgtccgggaa aggattttcc agatcgaggt ccacgggcat tccgaagccg ggaatgtgga
     2161 tgctgtcgcg actgatcgtc aggtcctcgc cgtgaacgac gccttcgccg aactgataga
     2221 tcggcttggc ttcgccgcga accatggcct cgtcgtagtc cagggcggcc tgctcgacgc
     2281 tttcgttgct gcggccgtgc tcggccaacg ccttcttgcc ccagcgcttg cgaagcatct
     2341 cccgggtctt cgccggtgac aggaacagcc cggtggcatt gttgccaccg aaacctttgg
     2401 agttgacgaa ggcggcatcc acgttctcgg cgccgagctc caggtgctcc agcggcaggt
     2461 tcagattgct gtcgtgaacg tcgtcggcga tgtgatcaat ggtggtgatg cctggcagcc
     2521 agccgtgctg ccacaggccc agggcggcgg ccagctgatc gcctcccgcg ggcgccatgg
     2581 agtggcccac gtaggccttg atggcagcta cgggccagcg ctcgataccg aagaccttgg
     2641 ccagctcgtt gagaatgtgg gactcggtca cccggttctg tggcgtcccg gtcccgtggg
     2701 ccagcatgta ggtgcggtgc tgcagggact cgtcgccgag cagcttgcgg gagaggctca
//
LOCUS       DBNHOX010000334         4094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1119719, whole genome shotgun sequence.
ACCESSION   DBNHOX010000334 DBNHOX010000000
VERSION     DBNHOX010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1119719"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="AC16UZ_07300"
     CDS             complement(<1..1398)
                     /locus_tag="AC16UZ_07300"
                     /inference="COORDINATES: protein motif:HMM:NF016032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HIR6246527.1"
                     /translation="MGDEIDRRYYDAEDFSDFRRRLDDETALLSDLFRRSEFSRRGDV
                     AGFELEVWLVDDEENPAPINDEYLKCLNNPLVVPELAEYNVELNGSPSALRGRVFSRL
                     HDELSATWRACRQAADRMGCALVTIGILPTVTEADLSSRYMSHMVRYQTLNDRVMALR
                     DGRPLELEIEGEGRLSTKHSDVMLEAAATSFQIHLQCRPDWAVRDFNASIVASAPIVA
                     ASANSPFLFGQSLWDETRIPLFEQAVDVGPNNPSRVSFGQGYIRESLLEIFEENRRDH
                     LILIPAVQDAPASKFSHVRFHNGTLWRWNRPLIGFDFDGQVHLRIEHRVVPAGPTIKD
                     CIANSAFYFGLIRGFGLQDEPVESRLPFEVARDNFYNCARYGLNGRVRWIRDGREEEL
                     LVRSLIMEDLLPLARRGLQSQDIPGEEIEAYLDVVAARVDSGQNGAAWQRRWVSLNGP
                     DLHGMVGAYRQQQDAG"
     gene            complement(1472..1723)
                     /locus_tag="AC16UZ_07305"
     CDS             complement(1472..1723)
                     /locus_tag="AC16UZ_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246528.1"
                     /translation="MDIDEVVSALRTERDELRVRLHLLKAEARDEFEELEKKWQHLES
                     RAAQVKEGAKESAEEIGAAAKTLAEEIGAAYTRIKKTLS"
     gene            complement(1756..2190)
                     /locus_tag="AC16UZ_07310"
     CDS             complement(1756..2190)
                     /locus_tag="AC16UZ_07310"
                     /inference="COORDINATES: protein motif:HMM:NF037928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HIR6246529.1"
                     /translation="MAQTWLIVADGARARFFERRSASDLSEFDVMISPEGRSEERSLH
                     SDRPGRSFDSRGDGRHAMEPAHSARDHEAEAFAKRVCKRLDEARLAGSVDKLVLIAPP
                     RFLGHLRASLSKGTQGIVAHSIDKELTQASPDEIAEHIPGVL"
     gene            complement(2266..3684)
                     /locus_tag="AC16UZ_07315"
     CDS             complement(2266..3684)
                     /locus_tag="AC16UZ_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR6246530.1"
                     /translation="MSDRAGSGTDVAELFSRFGVPGGAVVLVSPRGEESLTYGSVSVA
                     RPGPVGANTLFNVGSVAKTIVAAATGLLVAERRMQLDDRLVDWVPEFTFPEPHLRTRI
                     TLRDCLAHRLGLAREGFWEWGARESLELTTLLGRLRHASMARPYRSGYAYSNVGYAAV
                     ALAASRAAGMPFLDYLRLWLDLPGDACLTGGRAARESERLSACHVLTPGPRAVRPHFA
                     DNVLGGSGIWASADFARVWLRLQLGATPTSGRCRQLPGRIRRELRLPHQRVPLADRPF
                     GVGGEACSAVHYALGWFVTRYRGHRVLRHSGAEVGSSAHLAIAPDAGIGVFVMANAES
                     PAADVIGLTLIDHLLGLRPLPWATRAPMGTRAPNGSQPRLNVRSTRAHPDGLRGLTGT
                     FRSPANGPVRITVQHGRLQMAFRDAPRFDGELVPLAGGWFRPRPADPGVRSLMEEPVR
                     FEDRSDGSCALRLGGLGTWQRR"
     gene            complement(3686..>4094)
                     /locus_tag="AC16UZ_07320"
     CDS             complement(3686..>4094)
                     /locus_tag="AC16UZ_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246531.1"
                     /translation="ARNFLVAIALTFLFLGNAGCQSMDREEIAWQALHAVDVAQTMSA
                     ARDPCYREAAWLTRNIIGEQPSEVEVLAWGVGTAIIHRWVGQVLERRDAPRWVRVAWD
                     LGTIGYTGHTVVDNHRNGVRIWGDNVEHDGCSA"
BASE COUNT          716 a         1391 c         1306 g          681 t
ORIGIN      
        1 gccggcatcc tgctgctggc gataagcacc gaccatgccg tgcaggtccg ggccgttgag
       61 ggaaacccag cgacgctgcc aggcggctcc gttctgcccc gagtcgaccc gagccgccac
      121 cacgtccaga taggcttcga tctcctcccc cgggatgtcc tgtgactgca ggccacggcg
      181 cgccaagggg aggaggtctt ccatgatcag gcttcgaacc agcagctcct cctcacgtcc
      241 atcccgaatc cagcgcactc tgccgttgag cccatagcga gcgcaattgt agaagttgtc
      301 tctggcaacc tcgaacggca gccgcgactc caccggctca tcctgcaggc caaagccgcg
      361 aatcaggccg aaatagaatg cgctgttggc gatgcagtcc ttgatggtcg gtcctgccgg
      421 gaccacccgg tgctcgatgc gtaggtggac ctggccgtcg aagtcgaagc cgatgagcgg
      481 gcgattccac cgccacaggg tgccgttgtg gaaacgcacg tgactgaact tgctggccgg
      541 ggcgtcctga accgcgggga tcaggatgag gtggtcgcgg cggttctcct cgaagatctc
      601 cagcagggac tcgcggatgt aaccctgacc gaagctcacc cgggaggggt tgttgggtcc
      661 cacgtcgacg gcctgctcga agagcgggat gcgggtctcg tcccaaagag actgaccgaa
      721 aagaaacggc gagttggcgc ttgccgcgac gatgggcgca gaagccacga tgctggcgtt
      781 gaagtccctg acggcccagt ccggccggca ctgcaggtga atctggaaag acgtggcggc
      841 cgcttcgagc atcacgtccg agtgtttcgt ggaaagccgc ccttcaccct cgatttccag
      901 ctcgagggga cgcccgtcgc gcagcgccat gacccgatcg ttgagtgtct gatagcggac
      961 catgtgcgac atgtaccggg agctgaggtc ggcctcggtc accgtaggca ggatgccgat
     1021 ggtcaccaga gcgcagccca tccgatccgc ggcctgcctg caggcgcgcc aggtagcgct
     1081 cagctcgtcg tgcagccggg agaagacccg gccccggagc gcggacgggc tgccgttgag
     1141 ctcgacgttg tactcggcaa gctcaggaac gacgagcggg ttgttcagac acttgagata
     1201 ctcgtcgttg atgggcgcgg gattttcctc gtcgtccacc agccagactt cgagctcgaa
     1261 cccggccacg tcgccccggc ggctgaactc gctgcgacga aacagatcgc tcaacagcgc
     1321 ggtttcatca tcgagtcggc ggcggaaatc gctgaagtcc tcagcatcgt agtagcgtct
     1381 atcgatctcg tcgcccatgt aaatcccgcg tctcgagctc agcctgacgg caaccgctcc
     1441 gccgggccgg ggcggcaggt ccgtctatgt cctaggaaag cgtctttttg attcgcgtgt
     1501 aggcggcacc gatctcctcg gcaagcgtct tagctgcggc gccgatttcc tcggcggact
     1561 ccttcgcgcc ttcctttacc tgagccgcac ggctctcgag gtgctgccac ttcttctcca
     1621 gctcctcgaa ctcgtcgcga gcttcggcct tgagcaggtg caaccgcacc ctcaactcgt
     1681 cccgttccgt gcgcagggct gaaaccactt catcaatatc catgatcact cctaatagag
     1741 ttgttgtcag gcgccctaga ggacgcccgg tatgtgctcg gcaatctcgt cgggcgaggc
     1801 ctgcgtcaat tccttgtcga tgctgtgcgc gactataccc tgagtaccct tgctgaggct
     1861 cgctcgcagg tgccccagaa aacggggggg ggcgatgaga acgagcttgt ccaccgatcc
     1921 cgcaaggcgc gcttcgtcga gccttttgca gacgcgcttt gcgaacgcct ccgcttcgtg
     1981 atcccgggcg ctgtgggcgg gctccatggc atgccttccg tcaccgcggg aatcaaacga
     2041 tcggccggga cggtccgagt gcagcgagcg ttcttcggat cgcccctcgg gcgaaatcat
     2101 gacgtcgaac tcgctgagat ccgaagcgct cctgcgttcg aaaaaccgag cccgggcgcc
     2161 atccgcaacg atcaaccaag tctgtgccac gtaagcctcc cgtctttgaa cctgacgaga
     2221 ggttgcctgc gtcgtgccgc agtgtcttta aggatttccg cgtactcagc gccgctgcca
     2281 ggtaccgagc ccgccaaggc gcaacgcaca ggatccatca cttcgatctt cgaagcggac
     2341 cggttcctcc atgagcgagc gcacgcccgg atccgccggg cggggccgaa accacccgcc
     2401 ggccagcggc acgagctcgc catcgaatcg cggcgcgtca cggaaggcca tctgcagccg
     2461 cccgtgctgc acggtgatcc gcacgggccc gttggccggg ctccggaacg ttcccgtcag
     2521 cccgcgaaga ccatccggat gggcccgcgt ggagcgcacg ttcaggcgcg gctgcgagcc
     2581 gttcggcgct cgagttccca tgggcgcgcg ggtcgcccat ggcagagggc gcagacccag
     2641 cagatggtcg atgagcgtca gcccgatcac gtccgcggcc ggagattcgg cattggccat
     2701 cacgaacacg ccgatgcccg catcgggcgc gatggcgaga tgggcgctcg acccgacttc
     2761 agccccgctg tggcggagca cgcgatggcc gcgatagcgc gtgacaaacc accccagggc
     2821 gtaatgcacc gctgagcacg cttcgccacc gaccccgaat ggtctgtctg ccagcggcac
     2881 ccgctggtga ggcaggcgca gctcccgtct gatgcggccg ggcagctgcc gacacctgcc
     2941 gctcgtcggc gtcgcgccca gctgcaagcg cagccacacc cgggcgaagt ccgcggatgc
     3001 ccagattccg gagcccccca gaacgttgtc ggcgaagtgc gggcggaccg cacgaggacc
     3061 cggcgtcaga acgtggcagg cggagagcct ttcgctctcg cgagccgcgc ggcccccggt
     3121 caggcaagcg tctcccggaa gatccagcca gagccgaagg tagtccagaa acggcatccc
     3181 ggccgcccgg gaggcggcca gggccacggc tgcatagccg acgttcgagt aggcatatcc
     3241 ggaacgatag ggccgggcca tggaggcatg tcgcagtcgc ccgagcaggg tcgtgagctc
     3301 gagggactcc cgagcacccc actcccagaa cccttccctg gccagaccca gcctgtgggc
     3361 caggcagtcg cgcagcgtga tccgggtgcg aaggtgcggt tcgggaaacg tgaactcggg
     3421 aacccagtcc accagccgat catcgagctg cattcggcgc tcggccacca gcaagccggt
     3481 ggcagcggcc acgatggtct tggccaccga gccgacgttg aacagggtgt tcgccccgac
     3541 cgggccgggt cgggcgacgg atacgcttcc gtaggtgagc gactcctccc ccctcgggga
     3601 caccagcacg acggccccgc cgggaacgcc gaaccggctg aacagctcgg ctacgtctgt
     3661 cccgctccct gccctgtcgg acacgtcacg cgctgcaccc atcgtgctcg acgttgtcgc
     3721 cccagatgcg cacaccgttg cggtggttgt ccaccaccgt gtggccggtg tagccgatcg
     3781 ttcccagatc ccaggccacc cgcacccatc tcggcgcgtc gcggcgttcg agcacttgac
     3841 cgacccagcg gtgaatgatc gccgtgccca cgccccaggc cagcacctcg acctcgctgg
     3901 gctgctcgcc aatgatgttc cgggtcagcc atgcggcttc ccgatagcag gggtcgcggg
     3961 cggcgctcat ggtctgcgcc acgtctaccg cgtggagtgc ctgccaggca atctcttcac
     4021 gatccatgga ctggcagccc gcgtttccca gaaagagaaa cgtcagggcg atggcgacga
     4081 gaaaatttct cgca
//
LOCUS       DBNHOX010000335         1820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1440222, whole genome shotgun sequence.
ACCESSION   DBNHOX010000335 DBNHOX010000000
VERSION     DBNHOX010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1440222"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="AC16UZ_07325"
     CDS             complement(<1..623)
                     /locus_tag="AC16UZ_07325"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR6246532.1"
                     /translation="MRENALLAAWREGRQTIGCWLSMGNAYSAEALGQLGFDYVCIDL
                     QHGLVDYQDLTYMLPAVSRGTSTPLVRVPWNEPYEIMKALDAGAYGVIVPMVNNAEDA
                     ARAVDACRYPPLGSRSFGPIRGALYGGRGYAAQANEQMACIVMVETREGLDKLEEIVT
                     TPGVDGVYIGPADLALALGLPALGDTDVPEHVAEVTRILEVCHRHELP"
     gene            741..1793
                     /locus_tag="AC16UZ_07330"
     CDS             741..1793
                     /locus_tag="AC16UZ_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0003714 - transcription corepressor
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_function: GO:0140297 - DNA-binding transcription factor
                     binding [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HIR6246533.1"
                     /translation="MDRKKVVVQLPAISKAKAVEDRYALELEALAPLADIVEVDAQTP
                     AEFGEGASDADAIITSWGVKIDREVIGRLERCVVIGVGSVGVDMVDVDAATKAGIVVT
                     NVPDVFIEEVADHAMMLLLAAVRRTRLMGDLVRSDDWLQARPMLSKVPRLMGRTLGLF
                     SFGNVARCTARRARAFGLHVIAFDPYVSELAISDASVEPVSFAELLERSDYLSVHAPL
                     NDETRHVLDGDAFARMKPGAVIVNTARGPIIKEDDLIKALENGQIGAAGLDVLEREPP
                     DPANPLLDMANVIITPHVASASTRMRPETRRRVGREVALVLRGKWPMSCVNPTVLPRA
                     SLERWQPYPTQRGPNR"
BASE COUNT          316 a          614 c          589 g          301 t
ORIGIN      
        1 ggcagctcat ggcgatgaca gacctccaga atgcgggtga cttccgccac gtgctcgggc
       61 acgtccgtgt cgccaagcgc cggaaggccc agcgcaagcg ccagatcggc cggtccgatg
      121 tagaccccgt ccacgcccgg cgtggtcacg atctcctcga gcttgtccag gccctcccgg
      181 gtctccacca tcacgatgca ggccatctgc tcgttggcct gggccgcata gccacgcccg
      241 ccgtaaagcg ccccgcgaat gggcccgaaa gagcggctgc ccagcggcgg gtagcgacag
      301 gcgtcgacgg cccgcgccgc gtcttcggcg ttgttgacca tgggcacgat gacgccgtaa
      361 gcgccggcgt ccagcgcctt catgatctcg tagggctcgt tccagggtac gcgcaccagc
      421 ggcgtcgatg tgcctcggga cacggcgggc agcatgtagg tcagatcctg gtagtcgacc
      481 aggccgtgct gcagatcgat gcacacgtag tcgaagccga gctgtcccag cgcctctgcg
      541 gagtacgcgt ttcccatgga gagccagcag ccaatggtct gccggccttc ccgccacgct
      601 gccagcaatg cgttttcgcg cacgttcttc cctccccgga tctaccccca aaccttatca
      661 caggtaagga atgctgctga cactcgaaac gccggcgtgc gaaaatcggc gttctcattt
      721 gcacccggcg gagctcgacg atggaccgga aaaaggtggt cgttcagctt cccgccatct
      781 cgaaggcaaa ggcggttgaa gaccgctacg cgctcgagct cgaggccctg gcacctctgg
      841 cggacatcgt cgaggtggac gcgcaaacgc ccgccgagtt cggcgaagga gcgagcgatg
      901 cggatgccat catcacctcg tggggcgtga agatcgaccg ggaagtgatc ggccgtctcg
      961 agcgctgcgt ggtgatcggg gtgggaagcg tgggcgtcga catggtcgac gtggacgctg
     1021 ccacgaaggc cggcatcgtg gtgaccaacg ttcccgacgt gttcatcgag gaggtggccg
     1081 accatgccat gatgctcctt ctcgccgcgg tgcggcgcac gcgcctgatg ggcgacctgg
     1141 tacgaagcga cgactggctt caggcacgcc cgatgctgag caaggtgcct cggctcatgg
     1201 gccggacgct gggcctgttc tctttcggca acgttgcccg ttgcacggcg cgaagagccc
     1261 gggcgttcgg cctccacgtc atcgccttcg acccgtacgt gagcgagctg gcaatcagcg
     1321 acgcgagcgt ggagcccgtc tctttcgccg agctgctgga acgctccgac tacctgtccg
     1381 ttcacgcgcc gctgaacgac gagacgcgcc acgttctcga cggcgatgcg tttgctcgaa
     1441 tgaagcccgg cgcggtgatc gtgaacaccg cccgaggccc catcataaag gaagacgatc
     1501 tgatcaaagc cctcgagaac gggcagatcg gagccgccgg tctggacgtg ctcgaacgcg
     1561 agccgccaga tcccgccaac ccgcttctgg acatggccaa cgtcatcatc actcctcacg
     1621 tcgcctccgc ttctacccgg atgcgtccgg agacccgccg ccgtgtgggc agggaggtgg
     1681 ccctggtgct gcgaggaaag tggcccatga gctgcgtcaa ccccacggtg ctgccccggg
     1741 cgtctctcga gcgatggcaa ccctacccca cccagcgggg ccccaatcgg tgaggcggca
     1801 atcaccccgg acggctggct
//
LOCUS       DBNHOX010000336         3071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_797629, whole genome shotgun sequence.
ACCESSION   DBNHOX010000336 DBNHOX010000000
VERSION     DBNHOX010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_797629"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="AC16UZ_07335"
     CDS             complement(<1..663)
                     /locus_tag="AC16UZ_07335"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HIR6246534.1"
                     /translation="MSGACLQRYCETSLNYGDGDSARTVQVTIRNARTTPDLEFEECG
                     FSRLDHVSEVHDWSDAAHLADVHMAEVRELAQGFLGCDHALAYPPIVRSPASARAVAD
                     YAPIEFVHSDFTDDYLDMIRDPDRAYGAFLAPALETIGVRQDEIAKAERVVMLQFWRN
                     VGARRPDRPLALCDAASVPRSDLFSILVPEYGGQRLEFETFGVRCPKDPDRHRWYTYP
                     DLT"
     gene            complement(674..1360)
                     /locus_tag="AC16UZ_07340"
     CDS             complement(674..1360)
                     /locus_tag="AC16UZ_07340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR6246535.1"
                     /translation="MQKLYTWFAVIGFGSVAASLMVGFRHDPAAPAVNYLLNAGLFLA
                     YMVVHYVMMTPGFKKLVSSNPAGSPAERRLYMVVAIASWIAVYLIHYPMPGPALALPG
                     WVTFLGGCAFMMSFLAFTEGTTFEGIRAFAGVPGHEQTHGASAEAPLQTEGSYASVRH
                     PMYRGACYMGLTSLLLHPNAAQLAWAGIMGLTFLTFIPIEEKQLLKGRGDEYREYMKQ
                     TPYRIFRGVW"
     gene            1494..2834
                     /locus_tag="AC16UZ_07345"
     CDS             1494..2834
                     /locus_tag="AC16UZ_07345"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6246536.1"
                     /translation="MTIEIVNHTPEPAVIANYSTARYVDRDYMEREWEHVWRRSWLLA
                     GLESDVQKPGDYFVFDMGREQILVTRTASGNVQGFYNVCQHRGNLLVAGERGHADSFR
                     CAYHAWTYDTEGSLKIIPYQERFARGVPCAERSLRKVHTDVWDGFVFVSLAEEPEPLL
                     DFLGPVVEMLAPYRFRNMVLVEDQTVHHKCNWKAVVDNFSELYHVDFLHPQHKRMVDC
                     CNDTVHLFPRGHTGLAVPGATVNPRFPIPDEPTDLQSAQLSSIGLDPADFRGRVMDVR
                     EAVQKRKREVGAEMGFDYSVFSEDQLSDVWQYNLFPNIIFSFTPEHCWVMRPRPHPTD
                     PQQCYFDKLSLLLFADPEINSKATSIVGPGRPEAISGTHLPDDYVRPERDVFDHQAII
                     SGEKTMTITIDQDIELLGGVQTGMASAGFDTVWLNDDEMRVQHFHNELDRLIGS"
     gene            complement(2878..>3071)
                     /locus_tag="AC16UZ_07350"
     CDS             complement(2878..>3071)
                     /locus_tag="AC16UZ_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246537.1"
                     /translation="NRKIKDWFYPTFAKKIRAEQGDDRVQQFVEFLDSPRRVVVEFVP
                     TKKIVYDGAKMAAATPAIR"
BASE COUNT          599 a          979 c          938 g          555 t
ORIGIN      
        1 agtcaggtcc gggtacgtgt accagcgatg ccggtccggg tccttcggac agcgcactcc
       61 gaaggtttcg aactccagcc gctggccgcc gtactccggc accaggatgg aaaacagatc
      121 cgagcggggc acgctggccg cgtcgcacag ggcgagcggt cggtccggtc gtcgggcccc
      181 cacgttgcgc cagaactgca gcatgaccac gcgttccgct ttcgcgatct catcctgacg
      241 gacaccgatg gtctccagag ccggcgcgag aaaggcgccg taggctcgat ccgggtcccg
      301 gatcatgtcg agatagtcgt cggtgaagtc cgaatgaacg aactcgatgg gcgcgtagtc
      361 ggccactgcg cgggcactgg cagggctgcg gacgatcggc ggatacgcca gcgcgtgatc
      421 gcaaccgagg aacccctgcg ccagttctcg cacttcggcc atgtgaacgt cggcaaggtg
      481 ggcagcgtcg ctccagtcgt ggacctccga gacatgatcc agtcgggaaa aaccgcactc
      541 ctcgaattcg agatccggcg tcgttcttgc attgcggatc gtaacctgga ccgttcgggc
      601 cgaatcgccg tcaccgtagt tcagcgatgt ttcgcagtag cgctgcagac aagcgccgga
      661 catcgcgcgg cgcctaccag acgcctcgga atatgcggta aggcgtctgc ttcatgtact
      721 ccctgtactc atcgccacgt cctttgagca actgcttctc ctcgatgggt atgaacgtca
      781 ggaacgtgag ccccataatc cccgcccagg ccagctgcgc cgcgttggga tgaagcagca
      841 gggaggtcag ccccatgtaa cacgcgcccc ggtacattgg atgtctgacg ctggcgtagg
      901 aaccctccgt ctgcagcggc gcttcggcag aagcgccatg cgtctgctcg tgtcccggta
      961 ctcccgcaaa ggcccgaatc ccttcgaacg tggtcccttc tgtgaacgcg agaaaagaca
     1021 tcatgaacgc gcaacctcct agaaacgtca cccagcccgg aagcgccagc gcggggcccg
     1081 gcatcggata gtgaatcaga tacacggcga tccaggaggc gatggccacc accatgtaga
     1141 gcctgcgctc cgcggggctt cccgccggat tgctcgaaac cagcttcttg aacccgggcg
     1201 tcatcatcac gtagtggacg accatgtagg ccaagaacaa gccggcgttg agaaggtagt
     1261 tgacggccgg agctgcggga tcatggcgaa accccaccat gagagaggcg gcgaccgaac
     1321 cgaagccaat gaccgcaaac caggtataca gcttctgcat gctctcatcc cctcagaaaa
     1381 gagtttcccg aatatagccg aaggcgccgc gcagcgcctc cccgcaaggg aggcgtttcc
     1441 agccgcgacc gcggctggca tcattgggcg caggagaagc cagcggatcc gtcatgacca
     1501 tcgagatcgt caaccacacc cccgaacctg ccgtcatcgc caactactcg acggcccgct
     1561 acgtggaccg cgactacatg gagcgggagt gggagcacgt ctggcgcagg tcctggctgc
     1621 ttgccgggtt ggagagcgac gtgcagaagc ccggcgacta tttcgtcttc gacatggggc
     1681 gcgagcagat tctggtgacc cgaacggctt ccggcaacgt gcagggcttc tacaacgtct
     1741 gtcagcatcg aggcaacctg ctggtggccg gcgagcgcgg tcacgccgac agcttccgct
     1801 gcgcctacca cgcgtggacg tacgataccg aaggctctct gaagatcatt ccctaccagg
     1861 agcggttcgc cagaggtgtc ccgtgcgccg agcgatcgct gcggaaggtg cacacggacg
     1921 tatgggacgg tttcgtgttc gtctccctcg ccgaggagcc tgagccgctg ctcgattttc
     1981 tgggtccggt ggtggagatg ctcgcgcctt accgctttcg caacatggtt ctggttgagg
     2041 accagacggt acaccacaag tgcaactgga aggccgtcgt cgacaatttc agcgagcttt
     2101 accacgtgga ctttctgcat ccccagcaca aacgaatggt ggactgctgc aacgacacgg
     2161 tgcatctgtt tccccgcggg cacacgggcc tcgccgtgcc gggcgcgacg gtgaacccgc
     2221 gattcccgat ccccgacgag ccgacggacc ttcagtccgc gcagctatcg agcatcggcc
     2281 ttgatccggc cgatttccga ggaagggtga tggacgtgcg ggaggcggtg cagaagcgta
     2341 agcgcgaggt gggcgcggag atgggtttcg actactccgt tttctcggaa gatcagctct
     2401 ccgatgtctg gcagtacaac ctgttcccaa acatcatttt ctccttcacg ccggagcact
     2461 gctgggtaat gcgcccgcgg ccccacccga ccgatccgca gcagtgctat ttcgacaagc
     2521 tctcactgct gcttttcgcc gatcccgaga tcaactccaa agccacgtcg atcgtgggtc
     2581 ccgggaggcc ggaggcaata tccggcacgc acctgcctga cgactacgtc cgcccggagc
     2641 gcgacgtgtt cgatcatcag gccatcatca gcggcgagaa gaccatgacc atcaccatcg
     2701 atcaggacat cgaactgcta ggcggcgttc agaccggtat ggcgtctgcg ggtttcgata
     2761 cggtctggct gaacgacgac gagatgcgcg tgcagcactt tcacaacgag ctggaccgac
     2821 tcatcggcag ctgacatcgc ccggcgtggg ccttccgggg gcatctccga tggccgctta
     2881 ccgaatcgcc ggggttgccg ccgccatctt ggcaccgtcg tagacgatct tcttcgttgg
     2941 gacgaactcc acgacgaccc gccggggact atcgaggaac tccacgaact gctgcacgcg
     3001 gtcatcgccc tgttctgccc gaatcttctt tgcaaaggtc gggtagaacc agtccttgat
     3061 cttgcggttg c
//
LOCUS       DBNHOX010000337         4382 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_733832, whole genome shotgun sequence.
ACCESSION   DBNHOX010000337 DBNHOX010000000
VERSION     DBNHOX010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_733832"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="AC16UZ_07355"
     CDS             <1..940
                     /locus_tag="AC16UZ_07355"
                     /EC_number="2.7.-.-"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase enzyme family protein"
                     /protein_id="HIR6246538.1"
                     /translation="AGHINDTYLVTDGVDRWVLQRINDQVFTDPALLMRNLGRVVEHV
                     CARAPGFVPRLLSCRDGSNVFVDEEGGSWRLTEYVSGTESHRRLESVDQATSAARAFA
                     RYQNLLADLPAPPLDDPIPGFMRLDGYLAALDAATARAAGEHGAEDLLGFVEGRRDLA
                     QVLQDPGKTGGYIHGDCKINNLLFRENSAEVVCVVDLDTTMHGHWAWDFGDLARSGAV
                     TDAGALSLELFGALVEGFAGEKEAALGADELVLAPRYVCFMLGVRFLTDHLDGDRYFK
                     IAARGDNLRRARAQFRLLEEMEARESEMAEVAASRR"
     gene            complement(937..1182)
                     /locus_tag="AC16UZ_07360"
     CDS             complement(937..1182)
                     /locus_tag="AC16UZ_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HIR6246539.1"
                     /translation="MSIPTEDDHDPDPPERGPLTLTQTVASALAAAFGVQSSRNRTRD
                     FSRGRPAQFIAVGVVLTALLILGIAALVHLVLAGIES"
     gene            1262..1657
                     /locus_tag="AC16UZ_07365"
     CDS             1262..1657
                     /locus_tag="AC16UZ_07365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6246540.1"
                     /translation="MLAGLRESPSGFAMTRCAALILVAILLCGCAVSSNRPTQLVSGS
                     AAYPEDARAQGIEGLVVVRYDVAVDGRVINARVVRAEPPDVFDDAALASVRSWRFNPA
                     LADGVPRATQDIESTVTFKLDGGDEYADY"
     gene            complement(1742..2380)
                     /locus_tag="AC16UZ_07370"
     CDS             complement(1742..2380)
                     /locus_tag="AC16UZ_07370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6246541.1"
                     /translation="MEFDIAATDDLLATTRAVRKRLDLTRPVPREIINECLELAVQAP
                     TGSNSQGWRWVVVDDAAKRKALADLYREAAGGYLSGAEEQANESGNQQTQRVFSSAIY
                     LMEHLHEVPVHVIPCLEGRPPEDAPPITYAGVYGSIYPAVWSFQLALRARGLGSTLTT
                     LHLFKEREAAELLNIPDNVMQVALLPVGYTVGTDFKRADRPPVAGITHWNEW"
     gene            2482..3771
                     /locus_tag="AC16UZ_07375"
     CDS             2482..3771
                     /locus_tag="AC16UZ_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016982861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6246542.1"
                     /translation="MTLRRLAPLLLVLNAAGSLAEVSPEIRRGAEAVEAQVIAWRRDI
                     HAHPELSNREFRTAALVAEHLDSLGMEVTTGTAHTGVVGVLRGGLPGPVVALRADMDG
                     LPVEERTGLPYASKVRSTYNGLDVGVMHACGHDNHEAILMGAATVLSGMRERLPGTVK
                     FVFQPAEEGPPAGEDGGARMMVQEGVLEDPDVEAIFGLHISQADTVGFANYRPGGAMA
                     SAQRFEITITGRQTHGARPWQGVDPIVAGAHVVTALQTIVSRQVDISKAPAVVTVGSF
                     HGGVRNNIVPDSVKLEGTIRTFDADMRADIHSRIRRIATTVAESMGATADVYINDGVP
                     VTYNDPALTEAMLPTLHKVYGADRVRYALPITGAEDFSFFQEQVPGFYFFIGGRPADM
                     AAEDAIPNHSPLFFVDEGALKLGVQAMSQLAVDYLNR"
     gene            complement(3801..>4382)
                     /locus_tag="AC16UZ_07380"
     CDS             complement(3801..>4382)
                     /locus_tag="AC16UZ_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HIR6246543.1"
                     /translation="GPLEAGFADAAHRERWFAPSRSFDAALAESFAPLVATAAAGGLD
                     EWQQTPRGRLAYVLVCDQLPRNIYRGTARAFATDRRAVAAAREAVRIGVDLALALDER
                     AFLYLPFQHSEDILDQHLAVGLFTALRDASPKGYRDLTGGYLRSAQQHRDLIERFGRF
                     PHRNAALGRASTTEEAAYLEDGKTFGQAADAKG"
BASE COUNT          681 a         1437 c         1522 g          742 t
ORIGIN      
        1 tgccggacac atcaacgaca cgtacctggt gaccgacggc gtcgaccgct gggtcctgca
       61 gagaatcaac gaccaggtgt ttacggaccc ggccctcctg atgcgcaacc tgggccgggt
      121 agtggaacac gtttgcgctc gcgcgccggg attcgtgccc cggctgctgt cgtgccgcga
      181 tggctcgaac gtcttcgtgg acgaggaagg cggcagctgg cgcctgacgg agtacgtgag
      241 cggaaccgaa agccaccgtc gtctcgaaag cgtcgaccag gcaacgtcag cggcgcgtgc
      301 gttcgcccgt taccagaatc ttctcgccga tcttccggcc ccgccgctcg atgatccgat
      361 acccggcttc atgcggctcg acggctacct cgcagcgctc gatgccgcca cagcacgcgc
      421 ggcaggcgag cacggtgcgg aggaccttct cggattcgtg gaggggcgac gagatctggc
      481 ccaggtgctg caggatccgg gcaagacggg tggttacatt catggcgact gcaagatcaa
      541 caacctgctc tttcgtgaga acagcgcgga ggtggtctgc gtcgtcgatc tggacaccac
      601 catgcacggc cactgggcct gggacttcgg cgatctggcg cgctcaggcg cggtgacgga
      661 cgccggggcg ctgtctctcg agctcttcgg agcgctggtc gaggggtttg ccggtgagaa
      721 ggaagccgcg ctcggcgcgg acgagcttgt ccttgctccg cgttacgtct gcttcatgct
      781 gggcgttcgc ttcctcacgg atcatctgga cggcgaccgc tacttcaaga tcgccgcccg
      841 cggcgacaac ctccgccgcg cccgggcgca gtttcgcctg ctcgaggaga tggaggcgcg
      901 ggagtcggag atggccgagg tcgccgcgag tcgtcgctag ctctcgatgc cggcgaggac
      961 gagatgaacg agcgccgcga tgccgaggat cagcagggca gtgagcacca cgccgacggc
     1021 gatgaactgc gcgggccggc ctctgctgaa gtcgcgcgtc cggttgcggc ttgactgcac
     1081 gccgaaggcg gcggccagag cgctggccac ggtctgcgtc agggtcaggg ggccgcgctc
     1141 cggcgggtcg ggatcgtggt cgtcctctgt ggggatgctc atggggggag tatagcgccg
     1201 ggcgagcggg cggcagcgtc gtcgaccggc gcggtcttgc cagggccgga gcgaaggtgc
     1261 catgctcgcc ggcttgcggg aatcaccaag cggctttgcg atgactcgat gtgcggccct
     1321 cattcttgtc gcgatcctcc tctgcggatg cgcggtatcc tcgaaccggc cgacccagct
     1381 cgtctcgggc agcgcggcct acccggaaga tgcccgggcc cagggaatcg aggggctcgt
     1441 ggtggtccgc tacgacgtgg cggtggacgg ccgagtgatc aatgcccggg tggttcgcgc
     1501 cgagccgccg gacgttttcg atgatgcggc gttggcttcc gtgcgcagct ggcgtttcaa
     1561 ccccgctctt gccgatggcg tgccccgggc gacccaggac atcgagagca cggtgacctt
     1621 caagctcgac ggcggggacg agtacgccga ttactgatgg cgttcgggac caccgcctga
     1681 ccccgaagat cctttcaggg taaccgcgct cgtggtgctc gggctgggcg ccgcccggtc
     1741 gctaccactc gttccagtgg gtgatgcccg ccaccggcgg ccgatcggcg cgcttgaagt
     1801 cggtgccgac ggtgtacccc accggaagca gcgcaacctg catcacgttg tccggaatgt
     1861 tcagcagctc ggcggcctcc cgctccttga agagatgcag cgtcgtcagc gtggagccga
     1921 gcccccgcgc acgcagagcc agctgaaagc tccacacggc cgggtagatg gacccgtaga
     1981 cgcccgcgta ggtaatgggc ggagcgtctt ccggaggacg tccttccagg cacggtatga
     2041 cgtggacggg cacctcgtgg aggtgttcca tgaggtagat ggccgagctg aacacccgct
     2101 gggtctgctg gttgccggat tcgtttgcct gctcctccgc gcccgacagg tagccgcccg
     2161 cggcctcccg gtacaggtcc gccaatgcct tgcgcttcgc cgcgtcgtcg accacgaccc
     2221 agcgccagcc ctgggagttc gaccccgtcg gcgcctggac ggccagctcc aggcactcgt
     2281 tgatgatctc gcgcggcacg ggccgcgtca ggtcgagccg cttgcgcacg gcccgggtcg
     2341 tggccagcag gtcgtctgtg gcggcgatat cgaattccat gcttgttccc cctctgaatg
     2401 cccgggaagt ttaactcact ttgtgcgcat gcttgaggcg cattaagctc gcccaaaccg
     2461 gggacggctg aggaatccca gatgacgctg cgcagactcg ctcctctact tctcgtgctc
     2521 aacgctgccg gctccctcgc cgaggtctcg ccggagattc gccggggagc ggaagccgtg
     2581 gaggcccagg tcattgcctg gcgccgggac atacatgccc atccagagct ttccaaccgc
     2641 gagttccgca cggcggccct cgttgccgag cacctggaca gtctcggcat ggaggtgacc
     2701 accggcactg cccatacggg ggtggtgggc gtgctgcgag gcggactgcc aggtcccgtg
     2761 gtggccctgc gcgccgacat ggacggtctt cccgtagagg agcgcacggg attgccctat
     2821 gcctccaagg tccgctcgac ctacaacggc ctggacgtcg gggtgatgca cgcttgtggg
     2881 cacgacaatc acgaggccat cctcatgggc gcggctacgg ttctcagcgg catgcgcgag
     2941 cggctgcccg gcacggtcaa gttcgttttt cagccggccg aggagggccc gcctgccggt
     3001 gaggacggtg gggcgaggat gatggtgcaa gagggcgttc tcgaggatcc cgacgtggag
     3061 gccatcttcg gtctgcacat cagccaggcg gataccgtgg gttttgccaa ctatcggccc
     3121 ggcggcgcca tggccagcgc ccagcgcttc gagatcacca tcaccggccg ccagacccac
     3181 ggggcccggc cctggcaggg cgtcgacccc atcgtcgccg gtgctcacgt cgtcacggcg
     3241 ctgcagacca tcgtcagccg tcaggtggac atcagcaagg cgcccgcggt ggtcaccgtc
     3301 ggctcgttcc acggtggcgt gcgcaacaac atcgtcccgg actccgtcaa gctcgagggc
     3361 accatccgta ccttcgatgc cgacatgcgg gcggacattc attcaaggat ccgccgcatc
     3421 gcgaccaccg tggcggaatc catgggcgcg acggcggacg tctacatcaa cgacggcgtc
     3481 ccggtgacct acaacgatcc tgcgctaacc gaggccatgc tgcccaccct gcacaaggtc
     3541 tacggtgccg atcgggttcg ctacgcattg cccatcaccg gggccgagga cttctcgttc
     3601 ttccaggagc aggtgcccgg gttctatttc ttcatcggag ggcgccccgc tgacatggcg
     3661 gcggaggacg cgattcccaa tcactcgccg ctgttcttcg tggacgaagg cgcgctgaag
     3721 ctcggcgtcc aggccatgag ccagctcgcg gtggattatc tgaatcgctg atttacggca
     3781 aaggacaggc tgcaagagcg tcagcccttg gcgtcagcgg cttgtccgaa cgtcttgccg
     3841 tcctcgaggt aggccgcctc ttcggtggtg gaagcgcgtc cgagggcggc attgcggtgc
     3901 ggaaagcggc cgaatctctc gatgaggtcg cggtgctgct gagcggaccg cagatatccg
     3961 cccgtcagat cgcggtagcc cttggggctt gcgtccctca gggcggtgaa aaggcccacg
     4021 gccaggtgct ggtccaggat gtcctcggag tgctggaagg gcaggtacag gaaggcccgc
     4081 tcgtccaggg cgagggcgag atccacgccg atgcgaactg cctcccgggc ggcggccacg
     4141 gcccggcggt cggtggcgaa ggcccgggcc gtgcccctgt agatgttgcg cggcagctga
     4201 tcgcagacca gcacgtaggc caggcggccc ctcggcgtct gctgccactc atcgagcccg
     4261 cccgcggcgg cggtggccac cagcggcgcg aagctttcgg cgagggcggc gtcgaacgag
     4321 cggctcgggg caaaccagcg ctcccgatgg gccgcgtccg cgaagcccgc ctcgagcggt
     4381 cc
//
LOCUS       DBNHOX010000338        12031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_348294, whole genome shotgun sequence.
ACCESSION   DBNHOX010000338 DBNHOX010000000
VERSION     DBNHOX010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12031
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_348294"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="AC16UZ_07385"
     CDS             complement(<1..196)
                     /locus_tag="AC16UZ_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246544.1"
                     /translation="MEILRSALIGGVLAVAVLMPEFAVAEGEGAMIEELVVTARRRAE
                     SVQDVPGTVTAIGQSTIEGAG"
     gene            complement(268..816)
                     /locus_tag="AC16UZ_07390"
     CDS             complement(268..816)
                     /locus_tag="AC16UZ_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6246545.1"
                     /translation="MAETAQTVPTLGVSSETMAAARQDIEYLRRWYAKATDLLGENTP
                     ESIAEGTKIYHRIFTADAYIRTSGATTQPFEATGPDAWAEVANTALESYVATQHLIGT
                     QIVDIHELTQDAGGTITSGRASMTSYLQAWHAGEENLWLFIGTYMDEVRFTPGVGWQI
                     HDMNLVQVSGEHRALGGPRPTP"
     gene            complement(890..1375)
                     /locus_tag="AC16UZ_07395"
     CDS             complement(890..1375)
                     /locus_tag="AC16UZ_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6246546.1"
                     /translation="MIRGIHHVAVSTPDLDRIVRFYCDVMGAEQVYEGGWEKGSQLID
                     GIVGLKDSSCRQAMLKLGNAYLEFFEYQTPEPRPGDPARPVCDHGYTHFAIDVTDIDS
                     EYERLKAAGVKFNCPPPDFEGGPIRATYGRDPDGNVIEIQEIKDTAHEFYLDAWSGGR
                     S"
     gene            1469..2275
                     /locus_tag="AC16UZ_07400"
     CDS             1469..2275
                     /locus_tag="AC16UZ_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6246547.1"
                     /translation="MATRHYDTIEVESEGQVDWLTLNRPESLNAINTPMVTELRDYFG
                     GLQENADTRIVVMRGAGRAFCAGLDIKQARSGEHDNPFGAGMGFQGYLAEVYIRMRRC
                     PQPIISLIQGPACGGGFAFVLASDIRIAGESARMNAAFIRIGLSACDMGVSYFLPRLV
                     GTSVASELMLTGRFIHAARALATGLVSEVVPDAELVDAARPYIDEMLTTSPMGLRLTK
                     EGLNMAVDAASLEAAMAIENRNQVLCSKTSDAREGMQAFLEKRAPEYTGA"
     gene            2280..2903
                     /locus_tag="AC16UZ_07405"
     CDS             2280..2903
                     /locus_tag="AC16UZ_07405"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF036807.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6246548.1"
                     /translation="MALSVIGAGYGRTGTLSLKAALEQLGYLKCHHMIEVLQSPAQIE
                     GWMAAARGERVNWDELFAGYLASVDWPSCHFYRELADYYPQAKVVLTVRDPHAWYESM
                     NATTLRVIRRSMAEHPDRPSLGTELVVKGAFGGNIDSAAHAVEIFEAHTAEVMANIPS
                     ERLLVFDVREGWEPLCNFLQRDVPAAAFPRVNSREEFDGIFFGNRAD"
     gene            2932..3759
                     /locus_tag="AC16UZ_07410"
     CDS             2932..3759
                     /locus_tag="AC16UZ_07410"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246549.1"
                     /translation="MSEKIVLVTGGAQGIGKGLARACLQRGASVVITNLDSEIAATTV
                     AELSEHGPIRAIRCDATDRNAVDALFDDVWATEGPVDLAFCNAGAGGMGQILETPMAD
                     VHHQFSTNLYSAIHLAQSYIPRLVEANRAGHVMFTGSENSLVLPRANADLAMGIYGGT
                     KHALLAVAEWLRYELRDTAVTVSLLMPGPVLTERLAATFDALADDPDNPEIRGRFPEH
                     VERTLRERFISTERCAEIALRGLAQGLFFIPTQAYIREDVDARYRELTAAFDALDLS"
     gene            complement(3823..4044)
                     /locus_tag="AC16UZ_07415"
     CDS             complement(3823..4044)
                     /locus_tag="AC16UZ_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246550.1"
                     /translation="MTIIEHPFTGAIYRMNDEGNILVTQGDRQGVFRPDGRWVSGEIR
                     EADPQMCVWVSNVPASPGESDWHVTSDKT"
     gene            complement(4041..5402)
                     /locus_tag="AC16UZ_07420"
     CDS             complement(4041..5402)
                     /locus_tag="AC16UZ_07420"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6246551.1"
                     /translation="MAEAVKDLDPDNMVRSPGLTYQQLLDQDTHPVPDVLRLQSPKDF
                     GDADISIERYISKEWHDREVERLWKRVWQFACREEDIPDPGDHYRYDIAGMSFLVVRT
                     ESGAIRTYPNACLHRGRMLKEFDGHASELRCPFHGFAWHLDGSLKDVPAKWDFPHIDD
                     DQFGLPEIPTAVWAGFVFINPDQQCAPFEDFISDMASQFERWDLGSLYKQAHVAKIMP
                     ANWKIAQEAFCEAYHVNGTHPQILRSLGDINSQVDVWENCARVITPSLTPSPLLEYDL
                     TDGDVVRAMLDIPEGQPVPELPEGMSPRAWSAAMARETLRPDAGDRVDEYCDAELVDN
                     LDYTVFPNFHPWGAFNRIVYRFRPNGNDHRSAIMECIMLAPFQGERPPPAKIRWLAED
                     EPWSSALGFLGRVFDQDAFNMPKVQQGLEATYKPGITLANYQESKVRWLHHKLTEWVE
                     DGE"
     gene            complement(5497..8583)
                     /locus_tag="AC16UZ_07425"
     CDS             complement(5497..8583)
                     /locus_tag="AC16UZ_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6246552.1"
                     /translation="MSRIAQWAIERPLYPWLIMAACFFGGLYGIETVGRLEDPNFPIK
                     TAYIITSYPGASAVEVEQEVTDVIEEALLELPYIDHIISKSVPGRSEVQVDLLEHYGE
                     GDVPQIWDELRRRVVEAAERMPPGAGLPLVEDDFGDVYGLLYAIAAPDYSPADIRDMS
                     RQISTTLKRVPGVAKVQTSGEIGEAIFVELNHERLVRLGLPVDAVFGSIAQENSVVEA
                     GSVSYGQRRIRIAPELAFDSVAAVGDMRVGRPGSVEIIRLGDIADISRGPVEVPLDII
                     RHNGRRVFTVGVSVVDGRNVVEVGEAVDARLEQMLGDLPLGVEVIPIYRQHVVVSQAI
                     DEFGRNLMLSVVTVVLALCVFMGWRAGTVVGSVLLLTVMGTICVMSFLGIELQRISLG
                     ALMIAMGMLVDNAIVVAEGMVIGVRRGFTPKEAAALSVRRTMFPLLGATVIGILAFAP
                     IGLSPDKSGYYLRSLFEVVAISLLLSWLLAITVVPLLGNYLLVGRETARPEELYSGWG
                     YAPYRALLGFGLRRAWLAVLVLLAVTFGCIWGFQYVKQGFFPGTNAPLFYVDYYLPQG
                     TDIHATSGGIENVERILRTYPEVTDISAFIGKGPSRFTSIVRPEQPNPAYAHLVVRIT
                     DVNLMDAVIRRARQAVAEQIFDADIQIRRGEFTSSGTSKIEARFSGPDPDVLRHLAEQ
                     ALEVYLAHDLVERKTDWRQPELQIVPRFDEDRARVAGVSRTDVSRALALATLGVEIGL
                     FRDGNKLLPIIARAPHEERLDVGALSDRLVWSPAQQAHIPMRQIVSSFELVAENNTIY
                     RRDRERTIQARANPPPGHNPTMVFNSIRPGVEAIELPPGYILEWGGEHESSEDAKRLL
                     SSKIPLTFGLMLFISILMFGKLRQPLVIWLTVPMTVCGVVVSLLVTDLTFTFTAFLGF
                     LSLSGMLIKNCIVLVDEIDKRMLEGAHSLTTIVEASVSRLRPVMLAAGTTIAGMSPLL
                     ADAFFREMAVCIMGGLTFATLLTLVAVPVFYRIAFRRQLSSAQQASPGVLAGQAGG"
     gene            complement(8587..9648)
                     /locus_tag="AC16UZ_07430"
     CDS             complement(8587..9648)
                     /locus_tag="AC16UZ_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6246553.1"
                     /translation="MRRFLILLVLVCAGCEPAAEIVVEEQAVRPARIFRVSSEGVTVE
                     NQFVARVEAAQTVDVSFEVSGPLAELPVREGQTIDKGTLVAALDPTDFQLAVREAEVQ
                     LKLARQDLQRKRKLLADRGISQSVVDDAQALHDLRSVRLAQAREALEDARIVAPFDAY
                     VARRYTDNYANVQAGDKIVRLNDLNEIHVVASVPESLAATGSADQLIGAHAVFPFRPD
                     QRYPLTFRENLGEADAVAQTYEVTFAMPPPPNLQILPGMTATLELEMMAAEGEGRTMN
                     IPTSALIADSDKRFIVWIYDPETQAVSKRTVEVGPAVGRGVAVVSGLANGELIVATGA
                     SQLQNGMKIRVLGDPLTEL"
     gene            complement(9645..10193)
                     /locus_tag="AC16UZ_07435"
     CDS             complement(9645..10193)
                     /locus_tag="AC16UZ_07435"
                     /inference="COORDINATES: protein motif:HMM:NF037679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6246554.1"
                     /translation="MSAAGLAKTKILSAAAAVVGRSGAAHLTIDAVAAEAKLSKGGVL
                     YHFPSKRAILEGMLDRLISRYSERISRFEAELGDEPDAAIRAVILADRGQDAEERSMA
                     LALLAAAAEDPELLAPARAVIGQWFEAVEQSSGNVQLGILLLLATEGLRFLDMLDLLP
                     AGGTRREQLGQTLLNVAEAGRL"
     gene            10320..11495
                     /locus_tag="AC16UZ_07440"
     CDS             10320..11495
                     /locus_tag="AC16UZ_07440"
                     /inference="COORDINATES: protein motif:HMM:NF015963.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HIR6246555.1"
                     /translation="MPKWNNWSGRLAAEPDQIAFVRSEDDAAALVAAAKRKGHAVRTR
                     GACHSHAPLLVTDGTLVDLSGLSGVISANMETRQAWVWAGTPIYALGRALHDSGLALG
                     NQGDIDRQAIAGATATGTHGTGAALASLSASVAGARMVLADGSILDCGPDAERELWEA
                     ARLNLGAMGIITRLQLTLREPYRLREHGWTEPFDSLLPQIPHLAAGNRHFEFFWFPHN
                     DGAIVKTINETDDDPEYPLAEEGSRCAWSYEVLPNSRVQPHTEMEYSVPADRGPECLR
                     AIRELILSRFPDVRWPVEYRLLAEDDVWLSTAYGRPTATVSVHQDVALDETPYFRACE
                     EIFRSFDGRPHWGKVHYLEPDVLASIHPRWQDWWQARDRADPDGLFLNDYLAAWRPR"
     gene            complement(11509..>12031)
                     /locus_tag="AC16UZ_07445"
     CDS             complement(11509..>12031)
                     /locus_tag="AC16UZ_07445"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246556.1"
                     /translation="VEEALGTVTILVNNAGIPDAQRAHKMSLDLINAVIDTNLRGPYI
                     LSCEVARRLIDAKRPGRMVNISSVGGFRYDGNGAALYSITKSAIARMSECLAVEWSRF
                     NINVNCIAPGAFRSEMMDGMMERMGDFSGSFPRKRLCDPAQLDSTLLYLCAPSSECVT
                     GTIIKVDDGQGSR"
BASE COUNT         2210 a         3973 c         3749 g         2099 t
ORIGIN      
        1 ccccggcccc ttcgatggtc gactgcccga tggccgttac cgtcccgggc acgtcctgaa
       61 cggactccgc ccggcgccgg gcagtcacca ccagctcctc gatcatcgcc ccttcgccct
      121 cggcgacagc gaactcaggc atgagcacgg ctaccgccag caccccgccg atcaacgcgc
      181 ttcttaagat ttccatcgtt tcttaccccc tattgaccct tgtgattctg accctcgtca
      241 ttcgggatca ggaacaccgc tcctgaccta cggtgttgga cgcggccccc cgagcgcacg
      301 atgctccccc gaaacctgaa ccagattcat gtcgtgtatc tgccacccca cgcccggcgt
      361 gaaccgaact tcgtccatgt aggttccgat gaacagccac agattctcct cacccgcatg
      421 ccacgcctgc aggtagctgg tcatcgacgc ccggccggac gtgatcgtcc cgcccgcatc
      481 ctgcgtcagc tcgtggatgt ccacgatctg cgtgccgatc aggtgctgcg ttgccacata
      541 gctctccaga gcggtgttgg ccacttccgc ccaggcatca ggccccgtcg cctcgaacgg
      601 ctgggtggtg gcgcccgacg tcctaatgta ggcgtcagcc gtgaaaatcc ggtgataaat
      661 cttggtgcct tcagcgattg actccggcgt gttctcacca agcaggtcgg ttgccttggc
      721 ataccagcgc ctcaagtact cgatgtcctg ccgcgcagcc gccatcgtct cgctggacac
      781 cccgagcgtc gggacggtct gagccgtttc cgccatcaca cttcctccca tcaacgtagc
      841 cgcgacaata gcgcgaacgc ccgcaacttg gctagcaacg acatcgcctt caacttcgcc
      901 ctcccgacca ggcgtccagg tagaactcgt gcgccgtatc cttgatttcc tgaatctcga
      961 tgacgttgcc gtccggatcg cgtccgtagg ttgcgcgaat cggaccgcct tcgaaatcgg
     1021 ggggcgggca gttgaacttg accccggccg ctttgaggcg ctcgtactcc gaatcgatgt
     1081 cggtgacgtc gattgcgaaa tgggtatagc cgtgatcgca aaccggtctc gcaggatctc
     1141 ccggtcgggg ctcgggcgtc tgatactcga agaactccag ataggcgttg cccagcttca
     1201 gcatggcctg acggcaggac gagtctttca agccgacgat cccgtcgatg agctgggaac
     1261 ccttttccca acccccctca tagacctgct ctgcccccat gacgtcacag tagaaacgaa
     1321 cgatcctgtc cagatctggg gtcgacacgg cgacgtggtg aatgcctcga atcaattttc
     1381 atccccttca agtcgagatt cacggacatt aacattgaaa ggcgaggtcg gagaacatcc
     1441 gcaccggcct atcagcaggg agtcgagcat ggccacacga cactacgaca ccatcgaggt
     1501 cgaaagcgaa ggccaggtcg actggctgac gctgaaccgg ccggagtctc tgaacgccat
     1561 aaacacgccc atggtgactg agctgcggga ttatttcggc ggtcttcagg agaacgccga
     1621 cactcgtatc gtggtgatgc ggggcgccgg gcgcgccttc tgcgccgggc tggacatcaa
     1681 gcaggccaga tccggcgagc atgacaaccc cttcggggcc ggcatgggct tccagggtta
     1741 cctggccgag gtctacatcc gaatgcgccg ctgcccccag cccatcatct cgctgatcca
     1801 ggggccggcc tgcggcggcg gctttgcttt cgtgctggcc tctgacatcc gcatcgccgg
     1861 cgagagcgca cgcatgaacg ccgccttcat tcgcatcggc ctttcggcct gcgacatggg
     1921 cgtgagctac tttctgccac gactcgtcgg cacctcggtg gcctcagagt tgatgctgac
     1981 cggccggttc atccacgctg caagggcgct ggccacgggg ctggtatccg aggtggtccc
     2041 cgacgcggag ctcgtggacg cggccaggcc gtacatcgac gagatgctga ccacatcacc
     2101 catgggcctg cgcctgacca aggagggcct caacatggcg gtggatgcag ccagcctgga
     2161 agccgccatg gccatagaga accgtaacca ggtgctctgc tcgaagacca gcgacgccag
     2221 ggaaggtatg caggcatttc tggagaaacg ggcgcctgag tacaccggcg cttaggcggg
     2281 tggctctgtc tgtcatcggc gccggttacg gccggacggg aaccctgtcg ctgaaagccg
     2341 ctttggaaca gctcgggtac ctgaagtgcc accacatgat cgaggtgctg caaagcccgg
     2401 cgcagattga gggctggatg gccgcggcgc gcggcgagcg ggtcaattgg gacgagctct
     2461 tcgccggcta cctggccagc gtcgactggc cctcctgcca cttctaccgg gagctcgccg
     2521 actactaccc gcaggccaag gtggtgctga ccgtgcgcga cccccatgca tggtacgaga
     2581 gcatgaacgc gacgacgcta cgggtcattc gtcgttcgat ggccgagcac ccggaccgcc
     2641 ccagcctggg caccgagctg gttgtgaaag gcgctttcgg cggaaacatc gacagcgccg
     2701 cccacgccgt cgagatcttc gaagcccaca ccgccgaggt gatggccaac attccctccg
     2761 agcggctgct ggtgttcgac gtccgcgaag gatgggagcc actgtgcaac tttctgcagc
     2821 gcgacgtgcc cgccgctgcc ttcccacggg tcaattcccg ggaagagttc gacggcattt
     2881 tcttcggcaa tcgcgccgat tgactgcaac cgaggtatga aaatggaatc gttgagcgaa
     2941 aaaatcgtcc tggtcaccgg cggcgcccag ggtatcggca aaggcctggc gcgcgcctgc
     3001 ctgcaacgcg gtgcgagcgt ggtgatcacg aacctggact ccgagatcgc ggcgacgacg
     3061 gtagcggagc tgtccgagca cggccccatt cgggccattc gctgcgatgc caccgaccga
     3121 aatgccgtgg atgccctctt cgacgacgtc tgggccaccg agggccccgt cgacctggcg
     3181 ttctgcaacg ccggcgcagg gggcatgggc cagattctcg agacccccat ggcggacgtg
     3241 caccaccagt tctccaccaa tctctactcg gccattcacc tggcgcagag ctacatccca
     3301 cgcctggtcg aggcgaaccg cgccggccac gtcatgttca ccgggtcgga gaactccctc
     3361 gtcctgccgc gggccaatgc ggatctggcc atgggtatat atggagggac caagcacgcc
     3421 ctgctggccg tcgcggaatg gcttcgctac gagttgaggg acacggccgt caccgtcagc
     3481 ctgctcatgc cggggccggt gctgaccgaa cggctcgcgg ccaccttcga cgccctggcc
     3541 gacgacccgg acaacccgga gatccggggc cggtttcccg agcacgtgga gcgcaccctg
     3601 cgggagcgtt tcatctcaac cgagagatgc gcggagatcg ctttgcgcgg cctcgctcag
     3661 ggactcttct tcatcccgac ccaggcgtac attcgagagg acgtggacgc acgctaccgc
     3721 gagctcactg ccgcgttcga cgccctggat ctgtcctgat ccgccctctt ctcacccgcc
     3781 atgcgcctgc gccacggggc gcggcggcga ttgctccggc ggtcaggtct tgtccgaggt
     3841 aacgtgccag tccgactccc cggggctggc cggcacgttg gacacccaga cacacatctg
     3901 cggatcggcc tcgcggatct ccccggagac ccatcgtccg tcaggccgga aaacaccctg
     3961 gcgatcgcct tgcgtgacca ggatgttacc ttcgtcgttc atccggtaga tggcaccggt
     4021 gaacgggtgc tcgatgatgg tcactcgccg tcctccaccc actcggtgag cttatggtgc
     4081 agccagcgca ccttggactc ctggtagttc gcaagggtga tgccgggctt gtaggtcgcc
     4141 tcgaggccct gctgcacctt gggcatgttg aacgcgtcct gatcgaagac ccggccgaga
     4201 aagccgagcg ccgatgacca gggctcgtct tcggccagcc agcggatctt cgccggaggc
     4261 ggtcgttctc cctggaacgg ggcgagcatg atgcactcca tgatggccga cctgtggtcg
     4321 ttgccgttcg gccgaaagcg atagacgatg cggttgaacg cgccccacgg gtgaaagttg
     4381 ggaaacaccg tgtagtccag gttgtctacc agctccgcat cgcagtactc gtctaccctg
     4441 tcgcctgcgt ccggacgcaa ggtctcccga gccatggccg cgctccaggc cctcggcgac
     4501 ataccttccg gcagctccgg gacgggctgg ccttccggga tatcgagcat ggcgcgcacc
     4561 acgtcgccgt cggtcaggtc atactcgagc aaggggctgg gagtcaggga gggcgtgatg
     4621 acgcgggcgc agttctccca gacgtccacc tggctgttga tgtcgcccag gctcctcaag
     4681 atctgcggat gcgttccgtt gacgtggtat gcctcgcaga aagcttcctg ggcgatcttc
     4741 cagttagccg gcatgatctt ggccacgtgg gcctgcttgt aaaggctgcc caggtcccag
     4801 cgctcgaact ggctggccat gtcgctgatg aaatcctcga acggcgcgca ttgctggtcc
     4861 gggttgatga agacgaaacc ggcccagacc gccgtcggta tctccggcag gccgaactga
     4921 tcgtcatcga tgtggggaaa gtcccatttc gcgggaacgt ccttgagcga cccatccaga
     4981 tgccaggcga agccgtgaaa cgggcagcgc agttccgagg catggccatc gaactccttg
     5041 agcatgcgcc cccggtgcag gcaggcattg ggatacgtcc tgatggcgcc gctttccgtc
     5101 cggaccacga gaaacgacat tcctgcgatg tcgtaacgat agtgatcgcc ggggtcggga
     5161 atgtcttcct cccgacaagc gaactgccac acgcgcttcc acagccgctc cacctcacgg
     5221 tcatgccact ctttggagat atatcgctcg attgagatgt cggcatcgcc gaaatccttc
     5281 ggcgactgca gcctcagcac gtcgggaacc gggtgggtat cctgatcgag cagctgctga
     5341 tatgtgaggc ccggcgagcg gaccatgttg tccggatcca gatctttgac ggcttcggcc
     5401 attgcgtttc cccaagaaga gtaagcgatc gggcgatgcg catcgccctc gcgctacaga
     5461 ttcgccgtgc tcaccacgag tgtcaagacg ctcccgtcag cctcctgcct ggccggccag
     5521 gacacccgga gaggcttgct gcgcggagga aagctgacgc ctgaaagcga tgcggtagaa
     5581 caccggcacg gccaccaacg tcaacaacgt agcaaacgta agccccccca tgatgcagac
     5641 cgccatctcc cggaagaagg cgtcggccag caacggcgac atgcccgcta tagtggtccc
     5701 ggcggcgagc atcaccggcc gcagccggct cacgctggcc tcgacgatgg tcgtcaggct
     5761 atgggcgccc tccagcatcc gcttgtcgat ctcgtccacg aggacgatgc agttcttgat
     5821 cagcatgccg gacagactca gaaaaccgag gaacgccgtg aacgtgaagg tgagatcggt
     5881 aaccagcagg ctgacgacga ccccgcagac ggtcatgggg acggtaagcc agatcaccag
     5941 gggctgtcga agcttgccga acatgagaat gctgatgaac agcatgaggc cgaaggtgag
     6001 cggaatcttg ctggacagca gtcgcttcgc gtcctcgctc gattcgtgct caccgcccca
     6061 ctccaggata tatccgggcg gcagctcgat ggcttcaacg ccgggccgta tggagttgaa
     6121 caccatggtg gggttgtgtc ccggcggcgg gttcgcccgc gcctggatcg ttcgctcacg
     6181 gtcgcgtcgg taaatcgtgt tgttctccgc caccagctcg aagctggaga cgatctggcg
     6241 catgggtatg tgggcttgct gcgcggggct ccacacgaga cggtccgaca gcgctccaac
     6301 gtccagcctc tcttcgtggg gcgcccgggc aatgatgggc agcagcttgt ttccgtcccg
     6361 gaagaggccg atctcgacgc ccagcgtggc cagcgcgagc gcgcgggaaa cgtccgtccg
     6421 ggagacgccc gccacccgcg cgcgatcctc gtcgaagcgc ggaacgatct gcagctccgg
     6481 ttgccgccag tccgtcttcc gttcaaccag gtcgtgggcg aggtatacct ccagcgcctg
     6541 ctctgccaaa tgcctgagca cgtccggatc cgggccggag aaccgcgctt cgatcttcga
     6601 ggtcccgctg gaggtgaact cgccgcgccg tatctggatg tcggcatcga aaatctgttc
     6661 tgccaccgcc tggcgggcac gtcgaatcac cgcgtccatc aagttgacgt cggtgatccg
     6721 caccaccaga tgagcgtacg ccggatttgg ttgctcaggg cgcacaatcg acgtaaagcg
     6781 ggatggtccc ttacctataa aagctgatat atcagtaact tctggatatg ttctaagtat
     6841 cctttcgacg ttttcaatac cgccggaagt ggcatgaatg tccgttccct gaggcaggta
     6901 gtagtccacg tagaacaggg gcgcgttggt gccggggaag aagccctgct tgacgtactg
     6961 gaatccccag atgcagccaa aggtcaccgc caggagtacc agcaccgcca accaggcacg
     7021 gcgcaggccg aagccgagaa gggcccggta cggcgcgtac ccccagcccg agtagagctc
     7081 ctccggccga gcagtttccc ggccgaccag gaggtagttg cccagcagag gcaccacggt
     7141 tatcgccagc agccagctca gcagcagcga gatggcaacc acctcgaaca gggagcgcag
     7201 gtagtagccg gacttgtcgg gcgagagccc gatgggagcg aaggcgagta tgccgatgac
     7261 ggtggcgccg agcagaggaa acatggttcg acggaccgac agcgcagcgg cttccttggg
     7321 ggtaaagccc cgacggacac cgatgaccat gccctcggcg accacgatgg cgttgtccac
     7381 cagcatcccc atggcgatca tgagcgcgcc cagggagatg cgctgcagct cgatgccgag
     7441 aaacgacatg acgcagatgg tgcccatgac cgtgagcagc agcacgctac ccaccaccgt
     7501 tcccgcgcgc catcccatga agacgcagag cgcgagcacc accgtgacga ccgacagcat
     7561 caggttgcgg ccaaactcgt cgatggcttg gctgaccacc acgtgctggc ggtatatggg
     7621 aatcacctcc accccgaggg gaagatcgcc cagcatctgc tccagccggg cgtccaccgc
     7681 ttcgcccacc tcgaccacgt tgcgcccgtc caccacggag acgccgacgg tgaatacccg
     7741 gcggccgttg tggcggatga tgtccagtgg aacctctacc ggtccccggg aaatgtctgc
     7801 gatgtcgccg agccggatga tctccacgga gcccggccgg cccacccgca tgtcgccgac
     7861 ggcggccacg gaatcgaagg cgagttccgg cgcgatgcgg attcggcgtt ggccgtagga
     7921 aacggatccg gcttccacca cgctgttctc ctgggcgatg ctgccgaaca ccgcatcgac
     7981 cggaaggccg agccggacca ggcgctcgtg attcagctcc acgaagatgg cctcgccaat
     8041 ctcgccgctg gtctgcacct tggccacacc cgggacgcgc ttgagcgtgg tgctgatctg
     8101 gcgggacatg tcacggatgt cggccgggct gtagtcgggc gctgcgatgg cgtagagcag
     8161 gccatagacg tcaccgaaat cgtcttctac gagcggcagc cccgcacccg gcggcatgcg
     8221 ctcggccgcc tcaacgactc ggcgcctgag ctcgtcccag atctgcggca cgtctccctc
     8281 gccgtaatgc tcgagcaggt ctacctgcac ttccgaccgg ccggggacgg acttcgagat
     8341 tatgtggtcg atgtagggaa gctccagcag ggcttcctcg atgacgtcgg tgacctcctg
     8401 ctccacctcc actgccgagg ccccggggta ggacgtgatg atgtaggcgg tcttgatcgg
     8461 gaaattcgga tcttcaagcc tcccaacggt ctctatgccg tagaggccac cgaagaagca
     8521 ggcggccatg atcaaccagg ggtagagcgg tcgttcgatg gcccattgcg cgatgcggct
     8581 catgattcag agctccgtca gcggatcgcc cagcacgcgg atcttcatgc cgttctggag
     8641 ctggcttgcg ccggtggcaa cgatcagctc gccgttggcc agcccggaaa ccaccgcaac
     8701 tcccctgccc acggccggac ccacctccac cgtccgtttc gacacggcct gggtttcggg
     8761 atcgtagatc cagacgatga agcgcttgtc ggaatccgcg atcagcgcag aggtgggaat
     8821 gttcatcgtc cgaccttcgc cctctgctgc catcatctcc agctcgagcg tggccgtcat
     8881 gcccggcagg atctgaagat tcggtggggg cggcatggca aacgtcacct cgtaggtctg
     8941 cgcaaccgca tcggcttcgc cgaggttttc cctgaaggtc agcggatagc gctgatccgg
     9001 acggaagggg aaaacggcgt gagcgccaat cagctgatcg gccgaaccgg tcgcagccag
     9061 gctttccgga acgctggcaa ccacatgaat ctcgttcagg tcattgagcc ggacaatttt
     9121 gtcccctgcc tggacgttgg cgtaattgtc cgtataccgg cgcgcgacgt acgcatcgaa
     9181 gggcgcgacg atgcgggcgt cctccaacgc ctcccgtgcc tgggcaagcc ttacgctgcg
     9241 cagatcgtgg agtgcctgag catcgtcgac cacggactga gagataccgc ggtccgccag
     9301 cagcttccgc ttgcgctgca gatcctggcg cgcgagcttg agctggactt cggcttcccg
     9361 caccgcaagc tgaaagtccg tggggtccag ggcagcgacc agggtgcctt tgtcgatggt
     9421 ctgtccttcc cgaacgggga gctcggcgag cggccccgac acctcgaagg acacgtccac
     9481 cgtctgcgcg gcttccaccc gagccacgaa ctgattctcc acggtgacgc cttcgctgga
     9541 aacccgaaaa atccgggccg gccgaacggc ctgttcctcg acgacgatct cggcagccgg
     9601 ttcgcaacca gcgcagacca gcaccagcag gatcaggaag cgcctcacag ccgtccggcc
     9661 tcggcgacgt tgagcaacgt ctgcccgagt tgctctcggc gggtgccgcc cgcgggcagg
     9721 aggtcgagca tgtccaggaa tcgcagacct tccgtggcaa gcagcaggag aatgcccagc
     9781 tggacgttgc cggacgattg ctccacggcc tcgaaccatt gcccgatgac ggcccgcgcc
     9841 ggcgccagga gctccgggtc ctcggcggcg gcagccagca gcgccagcgc catggagcgt
     9901 tcctcggcgt cctgcccgcg gtcggccagg atgaccgccc ggatggccgc atccggctcg
     9961 tcgcccagct cggcctcgaa acggctgatc cgctccgaat agcgggatat gagacggtcc
    10021 agcatgccct ccagaatcgc gcgcttgctg ggaaaatgat agagcacgcc gcctttggag
    10081 agcttcgcct cggcggcgac ggcatcgatg gtcagatggg cagcaccgct gcggcccacc
    10141 accgccgcgg cggcgctgag tatcttcgtt ttcgccagac ctgcagcact catggccgca
    10201 aacgtaccgc aaaaaaacta taccgtccag acggtatagt ttcgttgcgt agaatgcggc
    10261 gctaccgtat gcgcagatcc agccccacga cgggcagagg acacgcaaag gacacgcata
    10321 tgccgaagtg gaacaactgg tccggccgtc tcgcagccga gcccgatcag atcgcgttcg
    10381 ttcgcagcga agacgatgcc gccgccttgg ttgccgcagc aaagcggaag ggtcacgcgg
    10441 tgcgcacgcg gggcgcctgc cacagccacg ccccattgct ggtcacggac gggaccctgg
    10501 tggacctgtc gggtttgagc ggcgtgatct cggccaacat ggagacgcgc caggcgtggg
    10561 tgtgggcggg caccccgatc tacgccctcg ggcgagcgct gcacgactcg ggacttgccc
    10621 tgggcaatca gggcgacatc gaccgtcagg ccatcgccgg agccaccgca acgggcactc
    10681 acggcacggg ggccgctctc gccagcctgt ctgccagcgt ggcgggagca cgcatggttc
    10741 tcgccgacgg gagcatccta gattgcggac ccgatgccga gcgggagctg tgggaggcgg
    10801 cccgcctgaa tctgggcgcc atgggcatca tcacccggtt acagctgacg ctgcgcgagc
    10861 cttaccggct gcgggagcac gggtggacag agccgttcga ctcgctgctg ccgcagatcc
    10921 cgcaccttgc agcaggaaac cgccacttcg agtttttctg gtttccccat aacgatgggg
    10981 cgatcgtcaa gaccatcaac gagacggacg acgaccccga gtacccgctg gcagaggaag
    11041 ggtcccgctg cgcctggagc tacgaggtgc tgccgaactc acgcgtgcag ccgcacaccg
    11101 aaatggagta cagcgtcccc gcagaccggg gccccgagtg cctgcgcgcc atacgggagc
    11161 tcattctcag caggttcccg gacgtgcgct ggcccgtgga gtaccgcctc ctcgccgagg
    11221 acgacgtgtg gctgtccacg gcttacggcc gcccgactgc caccgtgtcc gttcaccagg
    11281 acgtcgcgtt ggacgagaca ccctacttcc gcgcctgcga ggaaatattc cgcagcttcg
    11341 acggtcgtcc ccattggggc aaggttcact acctggaacc cgacgtgctg gcgagcatcc
    11401 acccgcgctg gcaggactgg tggcaggccc gcgaccgagc cgatccggac ggcctgttcc
    11461 tgaacgacta cctcgccgct tggcggcccc gatagcgaag cgatccggtc agcgcgagcc
    11521 ctggccgtcg tccaccttga tgatggtgcc ggtgacgcac tccgatgacg gcgcgcacag
    11581 ataaagcagg gtgctgtcca gctgagccgg atcgcacagc cgcttgcgcg ggaagctgcc
    11641 ggagaagtcg cccatccgct ccatcatccc gtccatcatc tccgacctga aagcgcccgg
    11701 cgcgatgcag ttgacgttga tgttgaatcg agaccactcc acggccaggc actcgctcat
    11761 gcgcgcgatg gccgatttgg taatggagta aagggcagcc ccgttaccgt cgtagcgaaa
    11821 cccgcccacg gagctgatgt tgaccatgcg gccgggacgc ttggcgtcga tgagtcgacg
    11881 cgccacctcg caggaaagta tgtacggacc ccgcaggttc gtatcgatga cggcgttgat
    11941 cagatccagg ctcatcttgt gcgcacgctg ggcatccggt atgccggcgt tgttcacgag
    12001 tatcgtcacg gtgccgagcg cttcctccac c
//
LOCUS       DBNHOX010000339         3916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_91253, whole genome shotgun sequence.
ACCESSION   DBNHOX010000339 DBNHOX010000000
VERSION     DBNHOX010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_91253"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="AC16UZ_07450"
     CDS             <1..1098
                     /locus_tag="AC16UZ_07450"
                     /inference="COORDINATES: protein motif:HMM:NF048350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7507 domain-containing protein"
                     /protein_id="HIR6246557.1"
                     /translation="ALATVTIVTVNPTLEVTKTASTDSVSENGEEVTFTVTVKNTSVS
                     SDPVTIGSLGDSVYGDLTAMGCADPGTIQPGDTYTCEFTVWLDAGVCTSGAPCSETNT
                     VTASGSDDDGTPVSDSASATVTITNDPSSIDVSKTASRASVPESGANVTFTFTVTNTS
                     FSDTVTINSISDDVFGDLNGQGDCVTPGDLAPRGGSWSCSVTEWIGGAPDDEHTNVFT
                     ASGVDDDGENVQGNASATVNYDDVPPAATVALKVSALVTYDVEVRNDSAADELTIGSI
                     VDDHYKDLTDVNNLMIQSSTCASGIVLAKKGEAGDTYQCSFTAEPGCSPLANSATASV
                     TDDDSAVAVTATSGTVSVSVSGLGACEAPTQ"
     gene            complement(1179..2804)
                     /locus_tag="AC16UZ_07455"
     CDS             complement(1179..2804)
                     /locus_tag="AC16UZ_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6246558.1"
                     /translation="MSALLKVDHLVTRFESGGEATDAVRDVSFEIKPGETVCLVGESG
                     SGKSVTGLSILGLLPDSAVHPNGTITLSTSGRPEVSLLEAKEETLQKVRGARISMIFQ
                     EPMTCLNPVLTIGEQLVEPLELHLGLMGEAARNRAVDLLELVEMPEPERRLDEYPHRL
                     SGGQRQRVMIAMALACEPDLLIADEPTTALDVTIQAQILKLMADLQRKRNTALLFITH
                     DLSVVSEIADRVLVMQAGRIVESGTRDQVLFEPQHPYTKELLAALPANLERLAPPRER
                     TSEPLIRVQNLCVYFPVRKGVLQRHVDDFKAVDHVSLDINKGRVLAVVGESGSGKTTL
                     GRAILGLIKPTAGSVLFAGVDLAGLSRRALKPYRRELQIVFQDPQSSLNPRLSIASIL
                     TEPMAAHGIGGNQRERLERAADLMQKVDLDPMMLGRFPHEFSGGQRQRIGIARALALE
                     PKFIVCDEVTSALDVSVQARILKLLDQLKQELDLTYLFITHNIDVVRYFADDIAVMYQ
                     GTVVEQGLANEVCENPRHPYTKRLLAAVPRFER"
     gene            complement(2801..>3916)
                     /locus_tag="AC16UZ_07460"
     CDS             complement(2801..>3916)
                     /locus_tag="AC16UZ_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6246559.1"
                     /translation="DTLRNTGAKIGIAWVATVAVLAVFAPFIANSHPYLMRVEGMWSS
                     PLLAHLSWADVTMLVTFLAVVAAWRLRRRFGPGQLLVGVGVISLVTLLAAQQLVQVPR
                     AVIYDQYRVGLAEGGIETAYFAPVPFSPTDRSRDNPAPLHEPDSVHWFGTDEDGADVL
                     SRMIHACRIALGIGLVATGIALALGIVYGGIMGFFSGWLDIAMMRFLEIFESIPSLFL
                     LLTIVAFFGRNIYLIMVVIGLTSWPGYARFLRAEFLKLRNQDYVQAAEACGLPLASIL
                     FRHMLPNGVAPLLVAASFGIASAILAEATLSFLGLGLIDEPSWGAMLNQAVGPTGTFA
                     LWLATYPGLAIFFTVFAYNLIGEALRDAIDPHLEQRP"
BASE COUNT          700 a         1254 c         1344 g          618 t
ORIGIN      
        1 gccctggcga cggtgaccat tgtcaccgtc aatccgaccc tcgaggtgac gaagacggcg
       61 tccacggaca gcgtcagcga gaatggcgag gaagtcacgt tcaccgtaac ggtgaagaac
      121 accagcgttt cgagcgaccc ggtgaccatc ggatcgcttg gggacagcgt ttacggcgat
      181 ctgaccgcca tgggttgcgc cgatcccggc acgatccagc cgggcgatac ctacacctgc
      241 gagttcaccg tctggctgga cgcgggcgtc tgcaccagcg gcgcgccctg ctccgagacg
      301 aacacggtga ccgcctccgg cagcgacgat gacggcacgc cggtctcgga cagcgcgtcg
      361 gccacggtca cgatcaccaa cgacccgtcg tccatcgacg tgagcaagac ggcaagccgc
      421 gcatcggttc cggagagcgg cgccaatgtg actttcacgt tcacggtgac caacacgtcc
      481 ttctcggaca cggtgacgat caactcgatc tccgacgacg tcttcggcga cctcaatggc
      541 caaggcgact gcgtgacccc cggtgacctc gcgccgcgcg gcggatcctg gtcctgctcc
      601 gtgactgagt ggatcggtgg agcgcccgac gacgagcaca ccaacgtctt caccgccagc
      661 ggtgttgatg acgacggcga aaacgttcag ggcaacgcct cggcgacggt caactacgac
      721 gacgtcccgc cggcagcgac ggttgccttg aaagtctccg ccctcgtcac ctacgacgtc
      781 gaggtccgaa acgactccgc ggcagatgag ctgaccatcg gctccatcgt ggacgatcac
      841 tacaaggatc tgacggacgt gaacaacctg atgatccaga gcagcacttg cgcctccggc
      901 atcgtgctag cgaagaaggg cgaagccggc gacacctacc agtgctcgtt cacggccgag
      961 ccgggctgct cgccgctggc gaactcggcg acggcaagcg tcaccgacga cgacagcgcc
     1021 gtggcggtta ccgccaccag cggcacggtc tcggtgtcgg tctccggcct gggcgcctgc
     1081 gaggccccga cccagtaacc accaaagggg acagcacctc cctttgcttt tgggagcgga
     1141 tgagaatccg ctcccttttt tttggcccgg tgtaggaact atcgctcgaa tctgggaacg
     1201 gccgccagca gccgcttggt gtagggatgc cggggatttt cacagacctc gttcgcaagg
     1261 ccctgctcca ccacggtgcc ctggtacatc acggcgatgt cgtcggcgaa gtagcgcacc
     1321 acgtcgatgt tgtgggtgat gaacagatag gtgaggtcca gctcctgctt gagctggtcc
     1381 agcagcttca ggatgcgcgc ctggacgctg acgtccagcg cgctggtgac ctcgtcgcag
     1441 acgatgaact tcggttccag cgccagcgcc cgcgcaatgc cgatccgctg gcgctggccg
     1501 ccggagaact cgtgggggaa gcggccgagc atcatgggat ccaggtccac cttctgcatg
     1561 agatctgctg cgcgttccag ccgctccctc tggttgcccc ctatgccgtg ggcggccatg
     1621 ggctcggtga ggatggaggc gatggacagg cgcgggttca gcgacgactg tgggtcctgg
     1681 aagacgatct gcagctcccg gcggtagggc ttgagcgccc ggcgcgagag accggccaga
     1741 tcgacgccgg cgaagagtac ggaccccgct gtgggcttga tgagcccgag gatggctctg
     1801 cccagggtgg tcttgcccga gcccgactcg cccaccacgg cgagcacccg gcctttgttg
     1861 atgtccaggc tgacgtggtc cacggccttg aagtcgtcga cgtggcgctg cagcacgcct
     1921 ttacggaccg ggaagtacac gcagaggttc tgcacccgga tgagcggctc ggacgtgcgt
     1981 tcccggggcg gtgcgagccg ctcgaggttt gcgggcaggg ccgccagcag ctccttggtg
     2041 tagggatgtt gcggctcgaa aagcacctga tcccgagtgc ctgactccac gatgcggccc
     2101 gcctgcatga ccagcacgcg gtcggcgatc tccgagacca cgctgagatc gtgggtgatg
     2161 aagagcagcg cggtgttgcg tttgcgctgc aggtcggcca tgagcttcag gatctgggcc
     2221 tggatggtca cgtccagagc cgtggtgggc tcgtcggcga tgagcagatc cggctcgcag
     2281 gccagcgcca tggcaatcat cacgcgctgg cgctgaccgc cggagagccg gtgcgggtac
     2341 tcgtccaggc gccgctcggg ctcgggcatt tccaccagct ccagcagatc cacggcccgg
     2401 ttgcgggccg cctcgcccat gaggccgaga tgcagctcca ggggttccac cagctgctcg
     2461 ccgatggtga gcaccgggtt caggcacgtc atgggctcct gaaagatcat ggagatgcgc
     2521 gcgccgcgca ccttctgcaa cgtctcctcc ttggcctcca gcagcgagac ctcgggcctg
     2581 ccgctggtgc tgagcgtgat ggtgccgttc gggtgcacgg cggaatccgg cagcaggccc
     2641 aggatggaga ggccggtgac gctcttgccg gagccggatt cccccaccag gcagaccgtc
     2701 tcccccggct tgatctcgaa cgagacgtcg cgaaccgcgt cggtggcctc gccccccgac
     2761 tcgaagcgcg tgaccagatg gtcgaccttg agcagcgcgc tcacggccgc tgctcgagat
     2821 gggggtcgat ggcgtcgcgc agggcttcgc cgatcaggtt gtaggcgaac acggtgaaga
     2881 agatggcgag cccgggatag gtggcgagcc acagcgcaaa ggtgccggtg ggcccgacgg
     2941 cctggttgag catggcgccc cagctcggct cgtcgatgag cccgagaccg agaaagctca
     3001 aggtggcttc ggccaggatg gccgaggcga tgccgaagct ggcggcaacc agcagcggcg
     3061 ccacgccgtt gggcagcatg tggcggaaca ggatcgaagc cagcggcaac ccgcaggctt
     3121 ccgccgcctg cacgtagtcc tggttgcgca gcttcaggaa ctcggcacgc aggaagcggg
     3181 cgtagccggg ccacgacgtc aggccgatga ccaccatgat cagatagatg ttgcggccga
     3241 agaacgccac gatggtaagc agcaggaaca gtgacgggat ggactcgaat atttcgagaa
     3301 agcgcatcat ggcgatgtcc agccagccgc tgaagaagcc catgatgccg ccgtaaacga
     3361 tgccgagggc gagcgcgatg ccggtggcca ccaggccgat gccaagggcg atgcggcagg
     3421 cgtgaatcat ccggctgagc acgtcggcgc cgtcctcgtc ggtgccgaac cagtgcacgc
     3481 tgtccggttc gtgcaggggg gcggggttgt cccgggaccg gtcggttggc gagaacggca
     3541 cgggtgcgaa gtaggcggtt tcgatcccgc cttcggccag gccgacccgg tactggtcgt
     3601 agatcaccgc acggggcacc tggacgagct gctgggcagc cagcagcgtc accaggctga
     3661 tcaccccgac gccgacgagc agctggccgg gcccgaatcg gcggcgcagg cgccaggcgg
     3721 cgacgacggc caagaaggtg acgagcatgg tgacgtctgc ccagctcagg tgggcgagca
     3781 gggggctgga ccacatgcct tccacgcgca tcagataggg gtgcgagttg gcgatgaacg
     3841 gcgcgaatac cgccagaacg gccaccgtgg cgacccaggc gatccctatc ttcgcgcccg
     3901 tgttgcgcag ggtgtc
//
LOCUS       DBNHOX010000340         1780 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_605823, whole genome shotgun sequence.
ACCESSION   DBNHOX010000340 DBNHOX010000000
VERSION     DBNHOX010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_605823"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="AC16UZ_07465"
     CDS             complement(<1..443)
                     /locus_tag="AC16UZ_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease family protein"
                     /protein_id="HIR6246560.1"
                     /translation="MSPVNQRRLANFRANRRGYWSLWIFLTLFVVSLAAELIANDKPI
                     LFVKGGELYVPVLFTYTEAEIGGELGTEAEYRDPYVQQLIEQDGWMFWPLIRYAHDTI
                     DWAVEVAPAPPSNEHWLGTDVFGSDMVAKLLYGFRLSVLFGLSLTL"
     gene            complement(440..1513)
                     /locus_tag="AC16UZ_07470"
     CDS             complement(440..1513)
                     /locus_tag="AC16UZ_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042884 - microcin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HIR6246561.1"
                     /translation="MGRYVLRRVLLILPTLFGIMVINFVIVQFAPGGPVEQALAQASG
                     FGMSSTASVSAGGAMGPAGDTGYTGGSGLDPALVERLEKQFGFDRPAPERFALMMWNY
                     VRLDFGSSYFQDRPVVDLVLERLPVSVSLGLWSLLLIYSISIPLGIAKAVRDGQRFDL
                     WTSAVVFTAYAVPGFLFAILLIVVFAGGSYLDWFPLRGLTSDDFSELSWPAKIADYFW
                     HLALPLTALTIGGFATLTMLTKNSFLEELNKQFVLTARAKGLGERRVLYGHVFRNAML
                     IVIGGFPAAFVGILFTGALLIEVIFSLDGIGLLGYEAVIKRDYPILFGTLFSFTLIGL
                     IMQLVGDLTYVLVDPRIDFEGRG"
     gene            complement(1514..>1780)
                     /locus_tag="AC16UZ_07475"
     CDS             complement(1514..>1780)
                     /locus_tag="AC16UZ_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6246562.1"
                     /translation="PAVDALIAHMYKAKTWEAFIAATRALDRVLLWNFYWVPGMSKTR
                     YALAYWNKFGQPAHGPLVRDTGFVDLWWWDQTRAARVAEFARSK"
BASE COUNT          363 a          568 c          587 g          262 t
ORIGIN      
        1 agggtcagcg acagaccgaa gagcaccgac agccggaagc cgtagagcag cttggccacc
       61 atgtcggagc cgaagacgtc ggtacccagc cagtgctcgt tgctcggtgg tgccggcgca
      121 acttccaccg cccagtcgat ggtgtcgtgg gcatagcgga tcaacggcca gaacatccag
      181 ccgtcctgct cgatgagctg ctggacgtag ggatcccgat actcggcttc ggttccgagc
      241 tcgccaccga tctccgcctc ggtgtaggta aagagcacgg gcacgtagag ctcgccgcct
      301 ttcacgaaca ggatgggctt gtcgttggcg atcagctcgg cagccagcga aaccacgaac
      361 agcgtgagaa atatccacag cgaccagtag ccgcgccggt tcgcccggaa gttggcgagc
      421 cgcctctggt tcacgggact catccgcggc cctcgaagtc gatgcgcggg tccaccagta
      481 cgtaggtgag atcgcccacc agctgcatga tgagcccgat cagggtgaac gagaacagcg
      541 tgccgaacag gatgggatag tcacgcttga tcacggcctc gtagccgagc agcccgatgc
      601 catccagcga gaaaatcacc tcgatcagca gcgcgccggt gaacaggatg ccgacgaacg
      661 cggcggggaa gccgccgatc acgatgagca tggcattgcg gaagacgtgg ccgtagagca
      721 cgcgccgctc gccgagcccc ttggcccgcg cggtgaggac gaactgcttg ttcagctcct
      781 cgagaaaact gttcttggtg agcatggtga gcgtggcgaa gccgccgatg gtcagcgccg
      841 taaggggcag cgccaggtgc cagaagtagt cggcgatctt cgccggccag gagagctcgg
      901 agaaatcgtc ggaggtgagt ccgcgcaggg ggaaccagtc gaggtaggag ccccccgcga
      961 agacgacgat cagcagtatg gcgaacagga acccgggtac ggcgtaggcc gtgaacacga
     1021 ccgcgctcgt ccacagatcg aagcgctggc catcgcggac cgctttggcg atgccgagcg
     1081 gtatggagat gctgtagatc agcagcagcg accagaggcc gagcgaaacg gacaccggca
     1141 gccgctccag gaccagatcc acgaccggac gatcctggaa gtagctggaa ccgaagtcca
     1201 gccgcacgta gttccacatc atgagcgcga agcgctccgg agcgggccgg tcgaagccga
     1261 actgtttctc gagccgctcg accagcgcgg gatccaggcc ggagcctccc gtgtagccgg
     1321 tgtcgcctgc cggtcccatg gcccctccgg cggacacgct ggcggtgctc gacatgccga
     1381 agcccgacgc ctgggccagc gcctgctcca ccgggccgcc gggcgcgaac tgcacgatca
     1441 cgaagttgat gaccatgatg ccgaacaggg tcggcaggat gagcagcact cgacgcagta
     1501 cgtagcggcc cacctacttg ctcctcgcga attcggcgac ccgcgctgcc ctggtctggt
     1561 cccaccacca gagatcgacg aagcccgtat cgcgcaccag cgggccgtgc gcgggttgcc
     1621 cgaacttgtt ccagtaggcg agcgcgtagc gggtcttaga catgcccggt acccagtaga
     1681 aattccacag cagcacccgg tccaatgccc gggtcgcggc gatgaacgcc tcccaggtct
     1741 tggccttgta catgtgagcg atcagcgcgt cgaccgccgg
//
LOCUS       DBNHOX010000341         4080 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1440655, whole genome shotgun sequence.
ACCESSION   DBNHOX010000341 DBNHOX010000000
VERSION     DBNHOX010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1440655"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="AC16UZ_07480"
     CDS             <1..309
                     /locus_tag="AC16UZ_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6246563.1"
                     /translation="AKTGYLVLRHMIPNTMGVILVTLTFAVPSAIFTEAFLSFIGMGV
                     APPTPSWGSMCNEGIRTMLTTPHELIFPALFISVTVLAFNLLGDGLRDALDARMRSTE
                     "
     gene            306..1331
                     /locus_tag="AC16UZ_07485"
     CDS             306..1331
                     /locus_tag="AC16UZ_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020289227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6246564.1"
                     /translation="MSTPRLLEVEDLKVEFDTYGGVVQAVRGVSFGVDEGETLAIVGE
                     SGCGKSVTVQSIMGLTPMPPGRITSGSARLRGTDIIGRKEIGGEDIRGGQIGMIFQDP
                     MTSLNPTMNIGSQIAETLEVHRGYSKKQAFQRAVELLDMSRIPEAASRARQYPFEFSG
                     GMLQRAMIAMAIACEPAILIADEPTTALDVTIQAQILDLMLDLQRETGMAIILITHDL
                     GVVARMADEVAVMYAGQIAEQGTVDDVFYRSSHPYTLGLRAAMPNNDPARQHELKPID
                     GSPPDLFHPPKGCAYAARCPYAMRICESEDPHAYQMTERHFARCWQHHEGAAARVEGL
                     YYREARA"
     gene            1328..2299
                     /locus_tag="AC16UZ_07490"
     CDS             1328..2299
                     /locus_tag="AC16UZ_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020357784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6246565.1"
                     /translation="MSERPVLLETDHLTKHFLIGRGQEVHAVDGVSLAIHEQEIVGLV
                     GESGSGKSTYGKTVVGLHDKTSGTVTYRGERLPQRYGAADFQKHARHMQMIFQDPYSS
                     LNPRMTVGEIIGEGLRLHANLDVREVRERVADWLKHVGLEPDHMSRDPHEFSGGQRQR
                     IGVARALILEPEFVVCDEPISALDVSVQAQVINLLDDLKRSMGLTMLFIAHDLSMVRY
                     VSDRMAVMYLGSVVEIGPANDVFFNPKHPYTEILVGSNPEPDPEAERHRASTAIVGEI
                     PSPVNVPEGCRFANRCPKVMDVCRSVTPELIPLKNAEAVREVACHLY"
     gene            complement(2524..>4080)
                     /locus_tag="AC16UZ_07495"
     CDS             complement(2524..>4080)
                     /locus_tag="AC16UZ_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6246566.1"
                     /translation="PNGANTAEFLTAIGAGALVPFIVEAAPGLGIAAYAGNPALGAYA
                     QGVPLLMPDGSYQSFDYSYGYDISSSDPEQFTQELRLASDFAGDLNFMLGGFYMDYET
                     ETHYVVRSTSLALIGQILPANPFLFPAPFGNPDDPQDEVNPYMQGYDNDTRKYELESW
                     AIFGELYWDITDSLSSTIGLRYSDEKKSSKQRTCYVTFLQCGPFDPEGENGYFFPKYD
                     DNEVTWKVNLTYDVNDDLMVYGTVSSSYKSGGFNPISDSDQLVIDDPSNATFEPETID
                     AFEVGFKSTLLDRTMQLNGSFFYYDYKDLQVSKIVDVTSLNKNTDAKILGAEVEVLWA
                     PIENLQFDLTGSWLDSEIKDFEDYDTADPKGDGSTDGLIVCLASVFLDTSPNGCGPGR
                     IPGVLQSQDGNQLAQSPEFSFNIGARYRVPMRGMGLTLATNFYWQDSFYVRNNNALHD
                     KVESWDVWNARARFAPNDERWYAEAWIKNILDNDYVVQQYLSTQVSGLFTNQFLLDPQ
                     TFGLTVGFNW"
BASE COUNT          753 a         1331 c         1282 g          714 t
ORIGIN      
        1 gcgaagaccg gctacctggt tctgcgccac atgattccca acaccatggg cgtcatcctg
       61 gtcacgctga ccttcgcggt gcccagcgcc atcttcacgg aggcgttcct gtccttcatc
      121 ggcatgggcg tcgccccccc gacgccgtcc tggggcagca tgtgcaacga gggcatccgc
      181 accatgctga ccacgcccca cgagctgatc tttcccgcgc tcttcatcag cgtgaccgtg
      241 ctggccttca acctgctcgg cgacggcctg cgcgatgccc ttgacgcccg catgaggtcc
      301 acggagtgag cacgccgcgg cttctggagg tggaggatct gaaggtggag ttcgacacct
      361 acggcggcgt ggtgcaggcc gtccgcgggg tgagcttcgg ggtggacgag ggcgagacgc
      421 tcgccatcgt cggcgagtcc ggctgcggca agtcggtgac ggtgcagagc atcatgggcc
      481 tgacccccat gccgcccggc cgcatcacct cgggcagcgc ccggctgcgc ggcacggaca
      541 tcatcgggcg caaggagatc ggcggcgagg acatccgcgg cggccagatc ggcatgatct
      601 tccaggaccc catgacgtcc ctgaacccca ccatgaacat cggctcccag attgcggaga
      661 ccctggaggt tcaccggggc tactccaaga aacaggcatt ccagcgggcc gtggagctcc
      721 tggacatgag ccggatcccc gaggcggcca gccgggcgcg ccagtacccg ttcgagttct
      781 ccggcggcat gctgcagcgg gcgatgatcg ccatggccat cgcctgcgag ccggccatcc
      841 tcattgccga tgagcccacc accgcgctcg acgtgaccat ccaggcgcag attctcgatc
      901 tcatgctcga cctgcagcgc gagaccggca tggccatcat cctcatcacc cacgatctgg
      961 gggtggtggc gcggatggcg gacgaggtgg cggtgatgta cgcgggccag atcgccgagc
     1021 aaggcaccgt ggacgacgtc ttctaccgct cctcccaccc ctacaccctg gggctccggg
     1081 cagccatgcc caacaacgac ccggcccgcc agcacgagct gaagcccatc gacggcagcc
     1141 cgcccgacct cttccacccg ccaaagggat gcgcctacgc cgcgcgctgc ccctacgcca
     1201 tgcgcatctg cgaaagcgaa gatccgcacg cctaccagat gacggagcgc cacttcgccc
     1261 gctgctggca gcaccacgaa ggcgccgccg cgcgcgtcga gggcctgtac taccgggagg
     1321 cgcgggcgtg agcgaacggc cggtgcttct cgagacggac catctcacca agcatttcct
     1381 gatcgggcga ggacaggagg tgcacgccgt ggacggcgtg agcctggcca ttcacgagca
     1441 ggagatcgtc gggctcgtcg gcgagagcgg atccggcaag tccacctacg ggaagaccgt
     1501 ggtggggctc cacgacaaga ccagcggcac cgtcacctac cggggcgaac ggctacccca
     1561 gcgctacggc gctgccgatt tccagaagca cgcccgccac atgcagatga tcttccagga
     1621 cccctactcg tcgctgaacc cgcgcatgac ggtgggcgag atcatcggcg agggcttgcg
     1681 gctccacgcc aacctggacg ttcgcgaagt gcgcgagcgg gtggccgact ggctcaagca
     1741 cgtggggctc gagcccgacc acatgtcccg cgacccccac gagttctcag gcggccagcg
     1801 ccagcgcatc ggcgtcgccc gggcactgat cctggaaccc gagttcgtcg tctgcgacga
     1861 gcccatctcc gcgctcgacg tctcggtgca ggcgcaggtc atcaacctgc tcgacgacct
     1921 gaagcgctcc atggggctca ccatgctctt catcgcccac gatctgtcca tggtgcgcta
     1981 cgtgagcgac cgcatggcgg tgatgtacct gggctccgtg gtggagatcg gccccgccaa
     2041 cgacgttttc ttcaacccga agcatccgta cacggagatc ctcgttggct ccaacccgga
     2101 gccggacccg gaggccgaac gccatcgggc gtcaacggcc atcgtcggcg agatcccttc
     2161 accggtgaac gtccccgagg ggtgccgctt cgccaaccgc tgcccgaaag tgatggacgt
     2221 gtgccgaagc gtcacgccgg agctgatccc gttaaagaac gcggaagccg tccgtgaagt
     2281 ggcctgtcac ctgtactgat agccgcctat ggacgccatc ttcatgatgc aagggccatc
     2341 aacatccgag gcaagcaaag gcccgcacaa gttgcgggct cgaatcgcgg gccgatgcag
     2401 cgggcgctgg gcacgatcgg ctttcgcaag tcgcgggcac aaaaaaaccc cgctggggct
     2461 cttaagccca gcggggtttc ggatcagcga gtgcccgtca tccaggcacc ctgaagatcg
     2521 tccctaccag ttgaacccga cggtcagacc gaaggtctgc ggatccagca ggaactggtt
     2581 ggtgaacagc cctgacacct gcgtggacag gtactgctgc accacgtagt cgttgtcgag
     2641 gatgttcttg atccaggctt ccgcgtacca gcgctcgtcg ttgggcgcga accgagcccg
     2701 cgcattccac acgtcccagg actccacctt gtcgtgcagg gcgttgttgt tgcgcacgta
     2761 gaaggagtcc tgccagtaga agttcgtcgc taacgtaagg cccatgcccc gcatgggcac
     2821 gcggtagcgg gcgccgatgt tgaagctgaa ctcgggcgac tgggcgagct gattgccatc
     2881 ctggctctgc agaacacccg gaatccggcc cggaccgcag ccgttggggc tcgtgtccag
     2941 gaacacgctg gcaagacaga cgatcaatcc atccgtgctg ccatctcctt tcgggtcggc
     3001 cgtgtcgtaa tcctcgaagt ccttgatctc ggaatcgagc caagagcccg tcaaatcgaa
     3061 ctgcaggttc tcgataggcg cccagagcac ctcgacctcg gccccgagga tctttgcgtc
     3121 ggtgtttttg ttgagcgagg tgacgtcgac gatcttggac acctgaaggt ctttgtaatc
     3181 atagtaaaag aatgagccgt tcagctgcat ggtcctgtcc agcagcgtcg acttgaagcc
     3241 aacctcgaaa gcgtcgatgg tctcgggctc gaaggtggcg ttggagggat cgtcgataac
     3301 cagctggtcg ctgtcgctga tggggttgaa gccgccgctc ttgtagctgg acgacacggt
     3361 tccatacacc atcaggtcgt cgttgacgtc ataggtgaga ttgaccttcc aggtgacctc
     3421 gttgtcgtcg tatttcggga agaagtagcc gttctcgccc tccgggtcga agggcccgca
     3481 ctgcaggaac gtgacgtagc acgtccgctg cttggagctc ttcttctcgt cggagtaacg
     3541 cagaccgatg gtcgagctca ggctgtcggt gatgtcccag tagagctcac cgaagatggc
     3601 ccagctctcc agctcgtact ttcgggtgtc gttgtcgtag ccctgcatgt aggggtttac
     3661 ttcatcctgt ggatcgtcgg ggttaccgaa gggcgcggga aacagaaacg gattcgcggg
     3721 cagtatctgg ccgatcagag ccagtgacgt gctgcgaacc acgtagtgcg tctccgtttc
     3781 gtaatccatg tagaagccgc cgagcatgaa attgagatcg ccggcgaaat ccgacgcgag
     3841 gcgaagctcc tgggtgaact gctcgggatc cgagctggag atgtcgtagc cgtaggagta
     3901 gtcaaagctc tgataacttc catccggcat cagcagcggc acgccctgag cgtaggcacc
     3961 cagtgccggg ttccccgcat aggctgcaat ccccagacct ggggcggctt caacgatgaa
     4021 cggcacgagc gcccctgccc cgatcgcggt cagaaattcc gccgtgttgg caccgttcgg
//
LOCUS       DBNHOX010000342         3053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_991656, whole genome shotgun sequence.
ACCESSION   DBNHOX010000342 DBNHOX010000000
VERSION     DBNHOX010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_991656"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="AC16UZ_07500"
     CDS             <1..689
                     /locus_tag="AC16UZ_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6246567.1"
                     /translation="KHDDLESIPAYEGIAWSYRLERFPPYFMSGGETRASEFSSAYEE
                     SISINNFPAGERALQQIIRIYRNTDSINPVVVSEAILELADWYLLFDKTKRAYPLYEY
                     VYDALEELEPGLAGTYFGEPKLLYFPVPQDPRPPDLQERGDARTGYVELAYEISDSGY
                     PRSLKTVASEPPGLMDFRVRKSLRLSRYRPMIVEGQALATQDFTYRYEFDYFEELEPA
                     QEVVEEEQDE"
     gene            complement(837..1043)
                     /locus_tag="AC16UZ_07505"
     CDS             complement(837..1043)
                     /locus_tag="AC16UZ_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246568.1"
                     /translation="MLLPLVGGAPPGWLGEDWGLCLPGFDGELGWLGELLGWLGELLG
                     EEGGVDGLGMLGGFGGWGGVWLLD"
     gene            1159..1857
                     /locus_tag="AC16UZ_07510"
     CDS             1159..1857
                     /locus_tag="AC16UZ_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246569.1"
                     /translation="MPGSPEGPDSGEPGEAGPGEKEPGVAGTEEAGSGQEQPGASGAG
                     DPGEDSPGWVDGDEAGEGGWETSNQVPAQTAEMPPMPSGSGGGPAEQAEPGQSEGDGE
                     LDKALESFDGEIMSEREVIMARSNENAGKTGSSIPMPGSGSGSGSQTGGSEGVPGAEQ
                     GGSDVPGGPAGMPRASSAPPPPMASAGDIPDDIPDAKDDDIIARQLREAAMKETDPEL
                     KEKLWDEYRRYKGA"
     gene            1903..>3053
                     /locus_tag="AC16UZ_07515"
     CDS             1903..>3053
                     /locus_tag="AC16UZ_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246570.1"
                     /translation="MKLLAVALLAGLCAGCVQEVVRTVDMTPPEQLDRVQTEDELLDI
                     GVAILDPNVPESYDEQIKQLVQPEIRRAEANFIPYVAKNLLQSTGNWGAVRVVPRPTH
                     AVDVVVEGKILHSTGENLAVEMSVHDATGRHWFSRRYEALASKYAYEEAIPQSIDPFQ
                     AIYKQLADDMLDYRKGLSYDEVMVIRRTAEMKFAQDFAPDAFADHITTTSEGEFELKR
                     LPAENDPMLSRVQRVREREYLFIDTLDEYYESFHRDMFESYQSWRRATYDEAIAYKAL
                     RAQSRARTIGGATAIIAGVAAMSESSDPYVDAGGIVSVIGGAMTIKGAIEKRAEAQIH
                     AEVLQEVGVAAEAEIVPYTIELENQTVRLQGSVDEQYEELRRILRRIYF"
BASE COUNT          623 a         1000 c          971 g          459 t
ORIGIN      
        1 gaaaacacga cgacctcgag tctatacccg cctatgaggg catcgcgtgg tcatatcgac
       61 tcgaacgttt tccgccctac ttcatgtccg gcggtgaaac cagggccagc gagttctcct
      121 ctgcctacga ggagtcgatc tccatcaaca atttccctgc cggcgaacgg gcgctgcagc
      181 agataatccg gatttaccgg aacaccgaca gcattaaccc cgtcgtggtc agtgaggcca
      241 ttctcgaact cgccgactgg tatctgctct tcgacaagac caaacgcgct tatccactct
      301 acgagtacgt ttacgatgct ctggaggagc tcgagcccgg tttggcgggg acctatttcg
      361 gcgagcccaa gctgctctat ttcccggtac cgcaggatcc ccggccgccc gatctgcagg
      421 agcgcggcga cgcccggacc ggctacgtcg agttagccta tgaaatctcg gacagcggct
      481 acccccgctc gctcaagacg gtggcatccg agcccccggg cctgatggat ttccgcgtcc
      541 gtaagagcct gcgcttgtcc agataccggc cgatgatcgt ggagggacag gctctggcga
      601 cccaggactt cacgtatcgc tacgagttcg actatttcga ggagctcgag cctgcccagg
      661 aggtcgttga ggaggagcag gatgagtaaa ccggctcgta cagccaccgt tcagcttcct
      721 cgcggtaggc tccccgcgtc tgcgcagcgg gatgtcggaa aggtgagcat gtccagatcg
      781 caacggcgtt cggcccgcac cctcgcgctg gccgtcgtgc ttgccttggc gggctgtcag
      841 tccagcagcc agacgccgcc ccagccccca aagccaccca gcatgccgag cccgtctaca
      901 ccaccctctt cgccgagcag ctcgccaagc cagcccagca gctcgccgag ccagccgagt
      961 tccccatcga aacccggcaa acagagcccc cagtcctctc ccagccagcc aggcggcgct
     1021 ccgccgacca gcggcagcag caccccgccc ccgggaggtc agcccagagc cggcgaggca
     1081 gacattcctc cgcctcccga cagcactgcg ggcaccggcg aagataccgg caccgagcag
     1141 ctgcccgaaa tgccgggaat gccgggatcc ccagaaggcc cggattccgg tgagcctggg
     1201 gaagccggac ccggcgaaaa ggagccgggc gtcgcgggca ccgaggaagc aggtagcgga
     1261 caggagcagc ctggcgcatc cggtgccggc gatcccggag aggattcgcc cggttgggtg
     1321 gatggcgacg aggcgggtga agggggttgg gagaccagca accaggtgcc agcgcagacg
     1381 gccgagatgc cgcccatgcc gtcaggttcc ggaggcggcc ccgcagagca ggccgaaccg
     1441 ggccaatccg agggtgacgg agagctcgac aaggcgctgg agagtttcga cggcgagatc
     1501 atgtcggagc gggaggtcat catggcacgc tccaacgaaa acgccggaaa aaccggctcg
     1561 tcgattccca tgccgggttc cgggagcggc agcggaagcc agaccggcgg gtcggaaggc
     1621 gttcccggcg cggagcaggg aggctccgac gtcccgggcg gccccgcggg tatgcccagg
     1681 gcgagctctg caccgccgcc tcccatggcc tcggcaggcg acatccccga cgacatcccc
     1741 gacgcgaagg acgacgacat cattgcacgc cagcttagag aggccgcgat gaaggagacg
     1801 gacccggagc tgaaggaaaa actatgggac gagtatcggc gctacaaggg cgcttgaacc
     1861 agcagtaggc aaatgcgtgt gcagccagac tcgaaagaaa gattgaagtt actcgcagtg
     1921 gcgttgctcg ccggtctctg cgccggctgc gttcaggagg tcgtccgaac cgtggacatg
     1981 acccctcccg agcagctcga tcgcgttcag accgaggacg agctgctcga catcggcgtg
     2041 gccattctcg acccgaacgt gccggagagc tacgacgagc agatcaagca gctcgttcag
     2101 ccggagatcc gccgcgcaga ggccaacttc attccctacg tcgcaaagaa cctgctgcag
     2161 agcacgggaa actggggggc cgtacgcgtc gtgccgcgcc cgacccacgc cgtcgacgtc
     2221 gtcgttgagg gcaagattct ccattccaca ggcgagaacc tggccgtgga gatgtcggtg
     2281 cacgatgcga ccggccgcca ctggttttcc cggcgctacg aagcgctggc cagcaaatac
     2341 gcctacgaag aggcgattcc gcagtccata gacccgtttc aggccatcta caagcagctg
     2401 gccgacgaca tgctcgacta tcgcaagggc ctgagctacg acgaggtcat ggtgatccgc
     2461 cgtaccgcgg agatgaagtt cgcgcaggat ttcgcgccgg acgcgttcgc cgaccacatc
     2521 accaccacct ccgagggaga gttcgagctc aaacgtcttc ccgctgagaa cgaccccatg
     2581 ctgagccgcg tccaacgcgt tcgggagcgc gagtacctct tcatagacac gctggacgag
     2641 tactacgaga gtttccaccg cgacatgttc gagtcctacc agagctggcg ccgagcgacc
     2701 tacgacgaag ccatcgccta caaggcgctg agggcgcagt cgcgcgctcg gaccatcggc
     2761 ggcgccacgg ccatcattgc cggggtcgcc gccatgagcg aaagcagcga cccatacgtg
     2821 gacgccggcg gcatcgtgag cgtcattggc ggcgccatga ccatcaaagg tgccatcgag
     2881 aaacgcgccg aggcgcagat tcacgcggag gtgctgcagg aagtcggtgt cgccgcggaa
     2941 gctgagatcg tcccctacac catcgagctg gagaaccaga ccgtgaggct ccaggggtcg
     3001 gtggacgagc agtacgaaga gctgcgtagg attctccgcc gcatttactt tga
//
LOCUS       DBNHOX010000343         1740 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1762257, whole genome shotgun sequence.
ACCESSION   DBNHOX010000343 DBNHOX010000000
VERSION     DBNHOX010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1762257"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1672)
                     /locus_tag="AC16UZ_07520"
     CDS             complement(<1..1672)
                     /locus_tag="AC16UZ_07520"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6246571.1"
                     /translation="MADAKQLLWGDTHLHTSYSFDAFLNNNLTADPDTAYRFAKGEPV
                     IHPYNRARVQLQKPLDFLVVSDHAEFIGGMLHVYRNGLDLDDAGIITQIEGWYRERQL
                     REAIDAGEGAPVFRSLLPAGSDDPRTAAATFGQSQAQQLPVSPEVFRSAWQEIVDTAE
                     AHYQPGKFTSLIGWEWSSVPGGANLHRIVVTDADAQTAKTFQPFSSLESPYPDDLWAW
                     LEKTSAATSAAFIAIPHNSNISKGWMFPRETLRGGPVDAAYGTTRMRWEPLVEATQIK
                     GDSETHPLLSPNDEFADFEEYSFYIEQQPGEYKPSEGDYVRPALKTGLELGEETGVNP
                     YQFGLIGSTDAHTGLSTAAEDNFWGKMATDSTPETKINTLITGGSRGWTMSASGMAAV
                     WAEENTREAIVDAMRRREVYATTGPRLRVRFFGGWEYDQADLEVEDLAAAGYAKGVPM
                     GGELTAAPENNGPRFMVVALKEPGGANLDRIQIVKGWLNAEGEAEERVYDVVWSDGRQ
                     PDADGKLTPVGNTVDLMTVRYTNDIGAVELASVWQDPDFDPGQSTFYYA"
BASE COUNT          277 a          558 c          581 g          324 t
ORIGIN      
        1 gagcgtagta gaaggtcgac tgccccgggt cgaaatccgg atcctgccag acggaggcaa
       61 gctcgaccgc accgatgtcg ttggtgtagc ggacggtcat cagatccacg gtgttgccta
      121 cgggcgtcag cttgccgtcc gcgtcgggct ggcgaccgtc agaccagacc acgtcgtaga
      181 cgcgttcctc ggcttcgccc tcggcgttca gccatccctt aacgatctga atgcggtcca
      241 ggttggcgcc gcccggctcc ttgagcgcta ccaccatgaa ccgcggccca ttgttctctg
      301 gcgcagcggt cagctccccg cccatgggaa cccccttggc gtacccggcg gccgccaggt
      361 cctcgacctc gaggtctgcc tggtcgtact cccagccgcc gaaaaaccgc acgcgcaggc
      421 gcgggccggt ggtggcgtag acttcccggc gccgcatggc gtcgacgatg gcctcgcggg
      481 tgttctcttc ggcccagacc gcggccatgc cggaggcgga catggtccaa ccccgggaac
      541 cgccggtgat cagcgtgttg atcttggtct ccggcgtgga gtccgttgcc atcttgcccc
      601 agaagttgtc ctctgccgcg gtggaaaggc ccgtgtgggc gtcggtggag ccgatcagcc
      661 cgaactggta ggggttcacg ccggtctctt cgccgagctc cagccccgtc ttcagcgcgg
      721 ggcgtacgta gtcgccctca ctgggcttgt actcgcccgg ctgctgctcg atgtagaagc
      781 tgtactcctc gaagtcggcg aactcgtcgt tgggcgagag cagagggtgg gtctccgagt
      841 cgcccttgat ctgggtggct tccaccaagg gctcccagcg catccgggtg gtgccgtacg
      901 ccgcgtcaac cgggcctccg cgcagggttt cccgggggaa catccagcct ttcgagatgt
      961 tcgagttgtg gggaatggcg atgaacgccg ccgaggtcgc agcactcgtc ttctccagcc
     1021 acgcccagag gtcgtccgga taagggctct ccagggagct gaaaggctga aaggtctttg
     1081 ccgtctgcgc atcggcgtcc gtcaccacga tacggtggag gttggcgccg ccgggcaccg
     1141 agctccattc ccagccgatc aggctggtga acttgcccgg ctggtaatgt gcctccgcgg
     1201 tgtcgacgat ctcctgccag gcgctgcgga acacctcggg ggatacgggc agctgctgag
     1261 cctggctctg gccgaacgtg gccgctgcgg tgcgcggatc atcggatccg gctggcagaa
     1321 gcgagcggaa aacgggcgcg ccctcgccgg catcaatggc ctcccgcaac tgccgttcgc
     1381 ggtaccagcc ctcgatctgc gtgatgatgc ccgcgtcatc caggtccagg ccattgcgat
     1441 acacgtggag catcccgccg atgaactcgg cgtggtcgga gacgacgagg aaatcgagcg
     1501 gtttctgaag ctgcacccgt gcccggttgt agggatgaat gaccggttcg cccttcgcga
     1561 accggtaggc cgtatccggg tcggccgtga ggttgttgtt caggaacgcg tcgaacgagt
     1621 acgacgtatg gaggtgggtg tcgccccaga gcagctgctt cgcgtcggcc acacctgggt
     1681 tgcacaaaag cccgatcagt gccatcaggg cggagatcag agtaatgaaa tttcgctgca
//
LOCUS       DBNHOX010000344         5410 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1056150, whole genome shotgun sequence.
ACCESSION   DBNHOX010000344 DBNHOX010000000
VERSION     DBNHOX010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1056150"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="AC16UZ_07525"
     CDS             <1..246
                     /locus_tag="AC16UZ_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246572.1"
                     /translation="GVDLDDVETVLRDGFVLNYLFACAIGHQASKYADPDRTQRFVMS
                     AALTLGNVDMLLEEHETRLRTSGYEEFVNRVKLELAN"
     gene            788..1852
                     /locus_tag="AC16UZ_07530"
     CDS             788..1852
                     /locus_tag="AC16UZ_07530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6246573.1"
                     /translation="MKAETLLPLGKLDPGLAAPEAALDLHAVAEDARTVDALGYWGLL
                     MEETKDDPFQVLAIAAAATSRVRLGTSVAIAFARSPFVTAMSAWTLQKLSNGRFELGL
                     GSQVRGHIRRRFGLQWHPPGPWMRDYVRAVRAIWASWQNGTKLDFSSQHYEIDLTVPL
                     FTPTPIDHPDIPIFVAAVNAYMCGVAAEVADGVRLHPVCSPRYIRDVVAPAVAEKRPA
                     GKPFEVCLKPLIAAADDGKTLSERAEVARQRLAFYVSTPAYRRPFENAGLGDLAAELS
                     RLSKEQRWHAMAERISDDVLEEWAVVATYDELAATIRSRFDGLVDRVEVGIPVASPED
                     RESLSRIVAELQSGGGSRAS"
     gene            1972..2988
                     /locus_tag="AC16UZ_07535"
     CDS             1972..2988
                     /locus_tag="AC16UZ_07535"
                     /inference="COORDINATES: protein motif:HMM:NF016241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HIR6246574.1"
                     /translation="MRHFPLLIAAMLTGILSSHAHATDFVMMKNGDRITGEIKQIWNG
                     EVFIEPEYGDTYPIDLDYVAYVHTDEDFEVEFVEGRRSRTVTGRLGLDDLGRPSVIVG
                     DAAETYPLAKIDNMQEIEDFFEWELATDISVNLATGNSDTRSSRFNVVGKMKLGEHRH
                     RLEYTRDQASAEGDTTKDQTYVAYEDVWTFRDDWFVRGAVNWTRDPIRDLASRSQIFL
                     GPGFHFWDDSKRTLNLSVGPNYLSEDIGGENKQSVAAQLVFRYEQHFLDDDLVVFQET
                     DYQTIVSGRKNKILNTSTGVRWDVLEDVYIKMQVDFDYETNPASAREKEDVTYLVGVG
                     LELD"
     gene            complement(3005..3877)
                     /locus_tag="AC16UZ_07540"
     CDS             complement(3005..3877)
                     /locus_tag="AC16UZ_07540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6246575.1"
                     /translation="MKVGLAFASSIGIDGATALDICRRAEAAGFESVWGGEHVIIPDA
                     IASKYPYTPDGKVPVEADTPIPDPLMWLAFAAAAAPTLRLGTCILIVPQRNPLILAKE
                     LATLDQLSGGRVELGLGVGWLKEEFEALGVPWERRGARNDEYIAAMRALWAGPHAEYH
                     GDFVDFAPVTCSPRPVNGSIPVIVGGDTDAAIARAVRLADGYFPGEGDAERLGALLGR
                     LRAAAEQAGRDPASIEVNAMFGVQMADPAAGIEQMAGLGVDRIMIPAFFFAGPDGLDR
                     LSEFGERVMPLTRG"
     gene            4027..4335
                     /locus_tag="AC16UZ_07545"
     CDS             4027..4335
                     /locus_tag="AC16UZ_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HIR6246576.1"
                     /translation="MTHVVTEACIGVKGEGCMEVCPEFCILSEPADHMSYIDPARCTD
                     CGVCVAACVVGAIFADKDVPRASREFININESWFRHKTGVRRRVRAMASEIGHWLPPE
                     "
     gene            complement(4384..5154)
                     /locus_tag="AC16UZ_07550"
     CDS             complement(4384..5154)
                     /locus_tag="AC16UZ_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246577.1"
                     /translation="MTMRNKILYTLGFALFVLVWLGVMDARADSHAAVAPSVLESYAC
                     SYKPGKDREDLDKLRDFYLKQAEKAGIAAPPAWLWTLNKGAAPVNFVWLNVHESLTAF
                     GGTDDAIAASPEMAEAFERADTVMTCQAGLGTFRQTYGGEMQESDSSVVSFGCGFREG
                     SGPATMDALHAQIADLNEMAGDLAVNSIFEVTPLTQGPGGSDIVMVVITDGTSGWARH
                     MDYINSIPAGLAAQSHFNAALDCNMNLWRLEQIVGGGD"
BASE COUNT         1075 a         1760 c         1632 g          943 t
ORIGIN      
        1 ggcgttgatc tggacgacgt agagaccgtt ttgcgggacg gcttcgtgtt gaattaccta
       61 tttgcctgcg ccatcggtca tcaggcttcg aagtacgctg atccggaccg tacccaacgg
      121 ttcgtaatga gtgcggcgct tacactgggg aacgtcgata tgctgcttga ggagcatgag
      181 actcgccttc gcacatcagg gtacgaggag ttcgtcaacc gagtgaagct cgaactggcc
      241 aactgacgac atcagcagat gccgcaaccg gtcagcgggt gcgcgcctat cttgatgagt
      301 tgcccgaaat tgaaacagcg ccggtcgcat tgaagctaag ttgatccgac cactgctggc
      361 gttggactaa accccgtcac ccacaaccgg agacgtccaa tgggtattca ggatctcggg
      421 gccatcgggg agttcatcag ctcgctcgtc atcgtcgtca cgctcttggt gctgatatcg
      481 cggcgcgaag aacgcgactc ttcaagctaa cgcacaggag cggcagccca agctagacgc
      541 cgtagatacc gatctctccc ggcagtccca ccagtcaact tctgaagtct acaaaagcgg
      601 gtggcgaggt tgttcagact attgaggtaa gagcaaaccc gccagatggg gggagatcac
      661 gggcgggcgc gtattaccgc cggcgccagg cgggaaggat cagccaatca gggcgtttcg
      721 acgacccccc accggttcgt tagactctcc ccgccttcac cacgtcgacc gggacgggga
      781 aaccgacatg aaagccgaga cccttctgcc gctgggcaag ctggatccag ggctcgcggc
      841 gcccgaggca gcgctcgacc tccacgccgt tgcagaagat gcccgcaccg tggacgcgct
      901 cggctactgg gggcttctca tggaggaaac caaggacgat ccgttccagg tgctcgccat
      961 agcggccgcg gcaaccagcc gcgtgcgcct gggcacctcc gtggccatcg ccttcgcccg
     1021 cagtccattc gtcaccgcca tgtcggcgtg gacattgcag aaactgtcca acggccgctt
     1081 cgagctcggc ctgggttctc aggtccgcgg ccacatccgc agacgcttcg gcctgcagtg
     1141 gcatcctccg ggaccgtgga tgcgcgacta cgtgagggcg gtgcgagcca tctgggcgag
     1201 ctggcagaac ggcacgaagc tcgacttttc gagccaacat tacgaaatcg acctgaccgt
     1261 accccttttt acgccgaccc ccatcgatca tcccgacatt cccatattcg ttgccgcagt
     1321 caacgcctac atgtgtggcg tcgccgcgga ggtggccgac ggggtgcgcc tgcatcccgt
     1381 ctgctcgccc cgctacatcc gggacgtggt ggcacccgcc gtggccgaga aacggcccgc
     1441 gggcaagccg ttcgaagtct gcctgaagcc gctgatcgcg gccgccgacg acgggaagac
     1501 gctcagcgag cgggcagagg tcgcccggca gcgcctggcc ttttacgtga gcacacccgc
     1561 ataccgccgt ccgttcgaga acgccggcct gggcgacctc gctgccgagc tcagccgcct
     1621 gtctaaggaa cagcgatggc atgcgatggc cgagcgcata agcgacgacg tgctggagga
     1681 gtgggcggtg gtcgccacct acgacgagct cgccgcaacg attcggtccc gcttcgacgg
     1741 cctcgttgac agggtggagg tcggcatccc ggtcgcaagc cccgaggaca gggagtcgct
     1801 gtcgaggatc gtcgccgagt tgcaaagcgg tggcgggagc cgcgcgagct gagccggggc
     1861 tcgtccaccg cacgagaatt ggacctttcc agcgaattgt ggcagaacac ccgcccctga
     1921 agagcgcgcc actcatcgcg ccgctgaatt cgtcgagctc gaggacccga catgcgacat
     1981 ttcccattgc tcatcgcagc aatgcttacc ggcattctga gcagccatgc ccacgcgacc
     2041 gacttcgtca tgatgaagaa cggcgaccga atcacgggcg agataaaaca gatctggaac
     2101 ggcgaggtct tcatcgagcc cgagtacggc gatacgtatc ccatcgacct cgactacgtc
     2161 gcttacgtgc acacggacga agacttcgag gtcgagttcg tcgagggccg gcgcagccgc
     2221 acggtgacgg gacggctggg gctggacgat ttgggccgtc cgtccgtcat cgtcggcgat
     2281 gcggcggaga cctacccgct ggccaagata gacaacatgc aggaaatcga ggacttcttc
     2341 gaatgggaat tggcgaccga catcagcgtc aacctcgcaa ccggaaactc ggacacccgc
     2401 tcctcccgct tcaacgtggt gggaaaaatg aagctggggg agcatcgaca ccggctcgaa
     2461 tacacccggg accaggcgag cgccgaaggc gacactacca aggaccagac atacgttgcc
     2521 tatgaggacg tctggacgtt ccgcgacgac tggttcgtcc gcggcgccgt caactggacg
     2581 cgcgacccga ttcgggacct ggcgagccga agccagatct tcctcggccc cggcttccac
     2641 ttctgggacg actccaagcg cacgctgaac ctcagcgtcg gccccaacta cctctcggag
     2701 gacatcggcg gggagaacaa acaatccgtg gcagctcagc tggtcttccg ctacgagcag
     2761 cacttcctgg atgacgatct cgtcgttttt caggaaaccg actaccagac catcgtgagc
     2821 ggccggaaga acaagattct caacaccagc acgggcgtgc gatgggacgt gctcgaagac
     2881 gtctacatca agatgcaggt ggacttcgac tacgagacca acccggcgag cgcccgcgag
     2941 aaagaagacg tcacctatct ggtcggcgtc gggctggagc tcgattgagt ccgctgaggc
     3001 gcgctcaacc ccgggtcagg ggcatcaccc gctcgccgaa ctcgctgaga cggtcaagac
     3061 catccggtcc ggcaaagaag aaggccggaa tcatgattct gtccacgccc agccccgcca
     3121 tctgctcgat gccggccgcc gggtctgcca tctgcacgcc gaacatggcg ttgacctcga
     3181 tgctggcggg gtcccgaccc gcctgctctg ccgcggcacg cagccgtccc agcaaggcac
     3241 cgagccgctc cgcgtccccc tcgccaggga aatagccgtc cgccaggcga accgcgcgag
     3301 cgatggccgc atcggtgtcg ccgccgacga tcaccggaat cgagccgttc acgggacgcg
     3361 ggctgcaggt cacgggcgcg aaatccacga aatccccgtg atactccgca tgtggtccgg
     3421 cccaaagcgc gcgcatggcc gcgatgtact cgtcgttgcg cgctccgcgc cgctcccagg
     3481 gcacgcccag ggcttcgaac tcctccttca gccacccgac gccgagacca agctcgaccc
     3541 ggccgccgga gagctggtcc agcgtcgcca gctccttcgc gagaatcagg gggttgcgct
     3601 ggggcacgat cagaatgcag gtgccgagac gcaacgtggg cgccgccgcg gctgcaaaag
     3661 ccagccacat gagggggtcg gggatcgggg tatccgcctc caccggaacc ttgccgtccg
     3721 gcgtgtaggg atatttggaa gcgatggcat cggggatgat gacgtgctca ccgccccaaa
     3781 ccgactcgaa tcccgccgcc tcggctcggc ggcagatatc cagagcagta gcgccatcga
     3841 tgccgatgct gctcgcgaag gctagtccga ctttcaccgt ttgattcccc tgctggtttg
     3901 aaaggcacat cctacaccgc ctcgttcgcc aggacccaat ctcttccggg tcccgatcgg
     3961 aaacccgcag ggtagccccg ggcttggggt aagatcggct gaccgcagac gaacggaaca
     4021 gaattgatga cccacgtcgt gactgaagcc tgcatcggcg tcaagggcga ggggtgcatg
     4081 gaggtgtgcc cggagttctg catcctcagc gagccggccg atcacatgtc gtacatcgat
     4141 cccgcccgct gcacggactg cggggtctgc gtcgccgcct gcgtggtcgg cgccatattc
     4201 gccgacaagg acgttcccag agcatcgcgg gagttcatca acatcaacga atcctggttt
     4261 cgccacaaga cgggcgtgcg caggcgcgtc agagcaatgg cttccgaaat tggccactgg
     4321 ctgccgccgg aatgaggcgc tccgcgagga gccgacccgc atggggtcgg ccccgcgtcg
     4381 agattaatct ccgccgccga cgatctgctc gagacgccag aggttcatgt tgcaatccag
     4441 agcggcattg aagtggctct gggctgcgag cccggcaggt atggagttga tgtagtccat
     4501 gtgacgggcc catccggacg tgccgtccgt gattaccacc atcacgatgt cggatccgcc
     4561 cggtccctgc gtcagcggcg tgacttcaaa gatcgagttg accgccagat cgccggccat
     4621 ctcattcaga tcagcgatct gggcgtgaag ggcatccatg gtcgccggac cggatccctc
     4681 tctgaatccg cacccgaatg aaacaacgct gctgtcactt tcctgcatct ctccaccata
     4741 cgtctgcctg aacgttccca gtccagcttg acaggtcatg acggtatctg cccgctcgaa
     4801 agcttccgcc atctccggcg atgcggcgat ggcatcatcg gtccccccga aagcggtaag
     4861 gctttcatgc acgttcaacc aaacgaagtt taccggtgca gcgcccttgt tgagggtcca
     4921 caaccatgca ggcggcgcgg caatgccggc cttttccgcc tgcttgagat agaagtccct
     4981 cagcttgtcc agatcttccc tgtctttccc tggcttgtag ctgcaggcat agctttccag
     5041 aacactcggc gccaccgccg catgggagtc agcccgagca tccatcactc ccagccagac
     5101 caacacgaac aaagcaaatc ccaacgtgta gagaattttg ttcctcatag tcaactgaac
     5161 ctcctgtgca cctcgaccat ccagccccgg ctacagtctc ccgtgacaac acctgcaggg
     5221 ggcaccctcc aggcaattta cgttagccgt cggccaacag cctgatgttc ttctgatggt
     5281 tcactgattt cgcgcccttc gctaagctca gcattcagcg ctgccatagc ggatcgacaa
     5341 gctacctgag acgggggaga cgggtgtcgg cggaatttca atttggcgac tggaccattg
     5401 agaccagcct
//
LOCUS       DBNHOX010000345         7224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_26293, whole genome shotgun sequence.
ACCESSION   DBNHOX010000345 DBNHOX010000000
VERSION     DBNHOX010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_26293"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="AC16UZ_07555"
     CDS             <1..668
                     /locus_tag="AC16UZ_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6246578.1"
                     /translation="QVAGCSLDEARAVLSTIAQFHADGWQPSPDRFGNVISHNNPAQR
                     DGMIGGWQVGWPVVKAQFPDLIPSAALEAGDRMPDAVGRLLEEMCREPVCLIHTDVRL
                     DNVFFGADEIMLVDWQAVCTSAPEQDVAYFVTQSVAPEVRAQDDLVKFYHGELVSHGI
                     DYGLERCRERYRVSALYLLCFAVVIAGTLDLANERGAQLGRTLLLNAFAALDEMDAFD
                     LLN"
     gene            691..1473
                     /locus_tag="AC16UZ_07560"
     CDS             691..1473
                     /locus_tag="AC16UZ_07560"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246579.1"
                     /translation="MSRLDGRVAVVTGGSRGIGAAIVRAFVDEGAMVAFNGRSAESGA
                     ALMEALGEPERVWFCQGSVTVTADLEKLIDGAVDRFGKLDILVNNAGGSNAMQPVAEL
                     DEAAWESDLRFNLTSTFLGTKLALKHMLPAGYGTVINISSVEGKMGVPVMAAYTACKH
                     GVNGFTKAVAAEVGRAGITVNAICPGLILTDAVTGGGPALAESMGMTFDEMVENVFKA
                     KTLTGELNTVEQIGETAVFLASPAGRGITGAQISVDGGMTPY"
     gene            complement(1506..2093)
                     /locus_tag="AC16UZ_07565"
     CDS             complement(1506..2093)
                     /locus_tag="AC16UZ_07565"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR6246580.1"
                     /translation="MSSEAGLRHLPNLISSLRLLCAPVLIWLTVQGLSDVFAWILIAA
                     WLSDLADGWLARSFGWVSRIGAMLDSVADIALTLATLYGIWVFHNDVFAVHGWLIWLV
                     LGLWVTVNSIALVRYGRPASFHARLAQVGMALFGVFVLTLFFYDFVGWIYYLCGAVCL
                     AAGVENLAMIYLLDDWTPNVRGGLMAALRRRQGDR"
     gene            complement(2090..5176)
                     /locus_tag="AC16UZ_07570"
     CDS             complement(2090..5176)
                     /locus_tag="AC16UZ_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6246581.1"
                     /translation="MNFTDTFVRRPVLASVISLLVLLVGIRAAGLLELREYPESNRAT
                     ITVTTAYPGADADLVQSFITVPLQRAISEAEGIDYLTSDSRQGVSIIEAFMELNYDPN
                     AAIAEIQAKVASQRNLLPQESMDPVIDSRTSLGWALMYLAFTSQEMSPAQVTDYLHRS
                     VIPRLQAIPGVAKAQINGNPTLAMRIWLNPHRMAALGVTATDVRAALQRNNFQAGVGE
                     TKGDYVRVTLSSTTGITAREDFARLIIRRDGETLIRLRDVAEVNLDSKNYDQTSWYNG
                     QPAILLALETSPGANPLTVSQAVRVLLPDIESQLPSNMEMQLAHDGSQYIDDSINEVL
                     RTLLEAMLIVLLVIFLSLGSWHAAVVPAVSVPLSLVGACALMLAFGFSLNLLTLLAML
                     LAIGLVVDDAIVVVENVHRHIAMNKTPFQAAIDGARELGLPILSMTTTLLAVYAPIAF
                     MGGLVGILFTEFAFSLAGAVLISGLVALTFSPMLSSKVLTSGEEPGRFESAVERFFAR
                     LAERYRARLSRALDYQPVTYLFAIVVMGSIYFMFTTSNTELAPTEDREVLNVQITAPE
                     TATLEYAVAHVRPAVKMFETFPEQRRTFLMIGGGGTPATSFGGFRFTPVAERERSQME
                     MQPILQRMLNEIAGVQIAVFSFPSLPGAARGTPLQFVIASDQDYRQLDTLADDVVQAA
                     YRSGNFLFLNKSVEFALPQTQIVIDRDRAGDLGIEMNDIGVALATLLGGNYVNRFSLA
                     GRSYEVIPQVERRFRASHEMLNDNYLRAASGELVPLSSFISFEHSIEPTRRSQFQQLN
                     AVTINGVMAPGVSLGDAITFMERTAAELFPREFSYDYVGESRQFKQQGSALVVTFFLS
                     IIVIYLVLAAQFESWRDPLIILISVPMSIAGALAFVMLGFASINLYTQVGLITLIGLI
                     AKNGILIVDFANRQQLVAGLSKRAAVEQAAAVRLRPILMTTVAMLMAMVPLLIATGPG
                     AVSRFQIGLVIATGLGIGTLFTLFVVPSVYLLLARDRTDPAQRDAATPRPTPGLEP"
     gene            complement(5173..6273)
                     /locus_tag="AC16UZ_07575"
     CDS             complement(5173..6273)
                     /locus_tag="AC16UZ_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6246582.1"
                     /translation="MAKRLIVMAGLLALVFGSLFGWKAFERHKTAEYLANARPPAVVV
                     SSTRVEEDRWRQQVESVGSLSAIQGVDVSSEVPGTVADISFQSGQQISAGELLVQLDA
                     TAERAELRSLEAQLVLARLDYERALGLLERTMLSQAQLDRAKSVMDSLQAQVEEQAAF
                     IAKKAIRAPFAGELGIRQVNLGEYVSPGTPIVTLQALDPIYANFALPERFLRELAVDQ
                     PLRVVVAAYPEDEFLGTVTAISPKVEETTRNVVVQATIANADRRLRPGMFARITVQTG
                     GDHRVLTVPRTAITFNPYGESVFLINEQDGALLVDRRQVITGEIRNERVEVVSGLLAD
                     DLVVATGQLKLRAGQRVKIDNSIDLSRPVTGP"
     gene            6405..6884
                     /locus_tag="AC16UZ_07580"
     CDS             6405..6884
                     /locus_tag="AC16UZ_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6246583.1"
                     /translation="MERTVKISQGRHAIEDIMYGYAEGIDTGNLETVAPLFAHGVMVM
                     PDGSELNGPEEVLRTFSDVIIFYDDEENVVPYQRGACSPRTKHVVTNSIFEFDNAVRN
                     ADVRSYFTVYQSLGGANEIIVGGRYIDQYELMIQGWVLVRREVYLENVGDLSRHLNG"
     gene            complement(6894..>7224)
                     /locus_tag="AC16UZ_07585"
     CDS             complement(6894..>7224)
                     /locus_tag="AC16UZ_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR6246584.1"
                     /translation="MVAEEQRRLGGNYRRVVLQGDISHPEVWRSLAASVDLSKDSPTV
                     ILGTGRAEDNLRTALWLKQSYPNAFVFARTNDKSELAVEVGSEHGIRNISIRQLVEDN
                     IPEEWLA"
BASE COUNT         1282 a         2252 c         2456 g         1234 t
ORIGIN      
        1 atcaggtggc gggttgcagc ctcgacgagg cgcgggcggt gctttccacg atagcgcagt
       61 ttcatgccga cgggtggcag ccctccccgg accggttcgg caacgtcatc tcccacaata
      121 accccgccca gcgcgacggg atgatcggcg gctggcaggt gggttggccc gtggtcaagg
      181 cccagtttcc ggatctgatc ccgtcggccg ccctcgaggc gggggacagg atgcccgacg
      241 ccgtggggcg gctgctggag gaaatgtgcc gggaacccgt ttgcctcatc cacaccgacg
      301 tgcgtctgga caacgtcttc ttcggcgctg acgagatcat gctggtggac tggcaggctg
      361 tgtgcacctc tgcccccgag caggacgttg cctacttcgt cacccaaagc gttgcgcccg
      421 aggtgcgggc ccaggacgat ctggtgaagt tctatcacgg cgagctcgtc agccacggca
      481 tcgactacgg cctcgagcgc tgccgggagc gttaccgggt ttctgccctg tatctgctct
      541 gcttcgccgt ggtcatcgcg gggaccctcg atctcgccaa cgaaaggggc gcccagcttg
      601 gccgaacgct gctgcttaac gcctttgcag cgctggacga gatggacgcc ttcgacctgc
      661 tgaactaacc gcgtttgccc gggaggtaac atgagtcgat tggacggccg ggtggccgtg
      721 gtcacgggcg gcagtcgcgg catcggcgcc gcgatcgttc gggccttcgt cgacgagggc
      781 gccatggtgg cgttcaacgg ccgctccgct gagtctggcg ctgcgctgat ggaagccctt
      841 ggtgagcccg agcgggtctg gttctgtcag ggctccgtga ccgtcacggc ggatctcgag
      901 aagctcatcg acggcgcggt cgatcgcttc ggcaagctgg acatcctggt gaacaatgcc
      961 gggggcagca acgccatgca gccggttgcg gagctggacg aggccgcctg ggagagcgat
     1021 ctgagattca acctcacctc gacgttcctg ggcaccaagc tggcgctcaa gcacatgctt
     1081 ccggccgggt acggcacggt catcaacatc tcctccgtgg agggcaagat gggggtgccg
     1141 gtcatggccg cctacacggc ctgcaagcac ggcgtgaacg gcttcaccaa ggccgtggca
     1201 gcggaggtgg ggcgtgcggg catcaccgtg aacgccatct gcccgggcct gatcctcacc
     1261 gacgcggtaa ccggcggcgg ccctgctctg gcggaatcca tgggcatgac gttcgatgag
     1321 atggtggaaa acgtcttcaa ggcgaaaacg ctgacgggtg agctgaacac ggtggagcag
     1381 atcggcgaga ccgccgtttt tctggcctca ccggccggcc gcgggatcac gggtgcccag
     1441 atctccgtcg acgggggcat gacgccctac tagcagcggc atcgggcggt tgccggtgcc
     1501 tgcaatcagc ggtcgccctg gcggcgccgc agcgcggcca tgaggccgcc tcgcacgttc
     1561 ggcgtccagt cgtcgagcag gtagatcatg gcgaggtttt ccacgcccgc agccaggcag
     1621 acggcgccgc acaggtagta gatccagccg acgaagtcgt agaagaagag cgtcaaaacg
     1681 aaaaccccga agagggccat gccgacctgt gcgagcctcg cgtggaagct ggccggtcgt
     1741 ccgtaccgga cgagcgcgat ggaattgact gtcacccaca ggccgagaac cagccagatc
     1801 agccagccgt gcacggcgaa gacgtcgttg tgaaagaccc agatgccgta aagcgtggcc
     1861 agcgtcaatg cgatgtcggc gacggaatcc agcatggcgc caatcctgct gacccagccg
     1921 aacgagcggg ccagccaccc gtctgcaaga tcgctgagcc aggctgcgat caggatccag
     1981 gcgaatacgt ccgacagccc ctgcaccgtg agccagatca gcacgggcgc gcagagaaga
     2041 cgcaggctcg agatcaggtt gggcaggtga cgcaggcctg cctctgaact catggctcga
     2101 gccccggcgt cgggcggggc gttgctgcat cgcgctgcgc aggatcggtt cggtcccttg
     2161 ccagcaaaag atagacgctc ggcacgacga acagcgtgaa caaggtgccg atgccgagcc
     2221 ccgtggcgat cacgaggccg atctggaagc ggctcaccgc ccctggcccg gtggcgatga
     2281 gcagcggcac catggccatg agcatggcta ccgtggtcat caggatcggc cgcaaccgga
     2341 ccgccgcggc ctgctccacc gctgcccgct tgctgagccc ggcgacgagc tgctggcggt
     2401 tggcgaagtc gacgatgagt atgccgttct tcgcgatcag gccgatgagc gtgatcagcc
     2461 ccacctgggt gtagaggttg atggaggcga atccgagcat gacgaacgcc agcgcgccgg
     2521 cgatggacat gggcaccgag atgaggatga tcagcggatc ccgccagctc tcgaactggg
     2581 ccgccagcac cagatagatg acgatgatcg acagaaagaa cgtcaccacc agagcgctgc
     2641 cctgctgctt gaactgacgg gactcgccca cgtagtcgta ggagaactcg cggggaaaca
     2701 gctctgcggc ggtgcgctcc atgaaggtga tggcatcccc gagggatacc cccggcgcca
     2761 tgacgccgtt gatggtcacg gcgttcagct gctgaaactg gctgcgacgg gtgggctcga
     2821 tggagtgctc gaagctgatg aagctcgaca ggggaacgag ctcgccggac gctgctcgca
     2881 ggtagttgtc gttcagcatc tcgtggctgg cgcggaagcg gcgctcgacc tgggggatca
     2941 cctcgtagct ccggcctgcg aggctgaacc ggttgacgta gttgccgccc agcagcgtcg
     3001 cgagcgcgac gccgatgtcg ttcatctcga taccgagatc acctgcccgg tcccgatcga
     3061 tcacgatctg cgtctggggc agcgcgaact ccaccgattt gttgagaaac aggaagttgc
     3121 cgctccgata agcggcctgc accacgtcgt cggccagggt gtccagctgc cggtagtcct
     3181 gatccgacgc gatcacgaac tgcagcggcg tacctctggc agcgcccggc agggagggga
     3241 acgagaacac ggcgatctgc acgccggcga tctcgttgag catcctctgc aggatgggct
     3301 gcatctccat ctgggaccgc tcgcgctccg cgacgggcgt gaagcgaaac ccgccgaagc
     3361 tggtggccgg cgtgccgccg ccgccgatca tcaggaaggt tcgccgctgc tctggaaacg
     3421 tctcgaacat cttcactgca ggccggacgt gtgccacggc gtattccagc gtggcggttt
     3481 ccggcgcggt gatctgaacg ttcagcacct cccggtcttc ggtgggcgca agctcggtgt
     3541 tgctggtggt gaacatgaag tagatgctgc ccatgacgac gatggcgaat aggtacgtga
     3601 cgggctggta gtcgagggct ctcgagagcc tcgcccgata gcgctcggcg agtcgggcga
     3661 agaaacgctc tacggcgctt tcgaatcgcc ccggctcctc gccgctggta agcaccttgg
     3721 aggagagcat gggcgagaac gtcaaagcca ccagccccga gatgagcacg gctccggcca
     3781 gggagaacgc gaactccgtg aagaggatgc ccaccagccc gcccatgaag gcgatggggg
     3841 cgtagacggc gagcagggtg gtcgtcatgg agagaatggg cagtcccagc tcccgggccc
     3901 cgtctatggc cgcctgaaac ggcgtcttgt tcatggcgat gtggcggtga acgttctcca
     3961 ccaccacgat ggcgtcgtcc accaccagcc cgatggcgag cagcatggcc agcagggtga
     4021 gcaggttcag cgagaagccg aaggccagca tgagcgcgca ggcgccgacc agggagagcg
     4081 gcacggagac ggcgggtacc acggccgcat gccaggagcc gagggaaagg aagatcacca
     4141 gcagcacgat gagcatggct tccagcaggg ttcgcagcac ctcgttgatg gagtcgtcga
     4201 tgtactggct gccgtcgtgg gcgagctgca tctccatgtt cgatggcagc tggctttcga
     4261 tgtccggcag caggacgcgg acggcctggg aaacggtcag aggattggcg cctggactgg
     4321 tctccagggc caggaggatg gccggttggc cgttgtacca gctggtctgg tcgtagttct
     4381 tcgaatccag attcacctcg gcgacgtcgc gcagccggat cagcgtctcg ccgtccctgc
     4441 ggatgatcag cctggcgaaa tcttccctgg cggtgatgcc ggtggtggag ctcagggtga
     4501 cgcgaacgta gtcgcccttg gtctcgccga cgccggcctg gaagttgttg cgttgcagcg
     4561 ccgcccgcac gtcggtcgcc gtgacgccca gggctgccat gcggtgcgga ttcagccaga
     4621 tgcgcatggc aagggtcggg tttccgttga tctgtgcctt ggcgacgccg gggattgcct
     4681 gcagccgcgg gatgacgctg cggtgcaggt agtcggtcac ctgagcgggg gacatctcct
     4741 ggctggtgaa ggcgaggtac atgagcgccc aacccaggct cgtgcgcgaa tcgatcaccg
     4801 ggtccatgga ttcctggggc aggaggttgc gctggctggc caccttggcc tggatctcgg
     4861 cgatggctgc gttcgggtcg tagttgagct ccatgaaggc ttcgatgatc gaaaccccct
     4921 gacgggaatc ggatgtcagg tagtcgatcc cctcggcttc tgagatggcg cgctgcaggg
     4981 gcacggtgat gaagctctgc accaggtccg cgtccgcgcc ggggtaggcg gtggtgaccg
     5041 tgatcgtggc gcggttgctt tccggatact cccgcagctc gagcagcccg gcggcccgga
     5101 tgccgaccag cagcacgagc aggctgatca cgctggccag caccggacgc cggacgaacg
     5161 tatcggtgaa gttcaagggc ccgtcaccgg cctggaaaga tcgatgctgt tgtcgatctt
     5221 cacgcgttgt ccggctctca gcttcagctg cccggtggcc accacgagat cgtcggccag
     5281 caggccggaa acgacctcca cgcgctcgtt gcggatctct ccggtgatga cctggcggcg
     5341 gtcgaccagc agagcgccgt cctgctcgtt gatgaggaag acggactcgc cgtagggatt
     5401 gaaggtgatc gccgttcgcg gaaccgtcag cacccggtgg tccccgccgg tctgcacggt
     5461 gatgcgcgcg aacattcctg gacggagcct gcgatcggcg ttggcaatgg tagcctggac
     5521 cacgacgttg cgcgtggtct cctcgacctt ggggctgatg gcggtcaccg tgccgaggaa
     5581 ctcatcctcc gggtaggcgg ccacgaccac gcgcagcggc tggtccacgg cgagctcgcg
     5641 caggaaccgt tccgggagcg cgaagttcgc gtagatggga tcaagcgcct gcagagtgac
     5701 gatgggcgtg cccggggaga cgtactcgcc aaggttgact tgccggatac cgagctcgcc
     5761 ggcgaagggt gcccgtatgg ccttcttggc aatgaaggcg gcctgctcct caacctgcgc
     5821 ctgcaggctg tccatcacgc tcttggcccg atcgagctgc gcctgtgaca acatcgtgcg
     5881 ctcaaggaga cctaaagcgc gctcgtagtc cagtcgcgcc agcaccagct gggcttccag
     5941 cgagcgcagc tcggcccgtt cggcggtggc gtcgagctgg acgagcagct cgcctgcgct
     6001 gatctgctgc ccggattgaa aactgatgtc cgcgacggtg ccgggcacct cggagctgac
     6061 gtccacgccc tggatggcgc tcaacgagcc gacggattcc acctgctgcc gccagcggtc
     6121 ctcttccacc ctcgttgacg agacgaccac cgccggcggc cgggcgtttg ccagatactc
     6181 ggccgttttg tggcgctcga acgccttcca gccgaacagg ctgccgaaga ccagcgccag
     6241 cagcccggcc atcacgatca gtcgcttggc catggcgcct cgatccttgc tctgcacatc
     6301 gatctggatc cccccggaag acgctgcatt atagcgccga ggcggcccgt ggctgtctcc
     6361 atggcgggtg aatgggcata atgtcgcctg gcacggaggg tgagatggaa cgcacggtca
     6421 agatttctca ggggcggcac gccatcgagg acatcatgta cggctacgcc gagggcatcg
     6481 acacgggaaa tctagaaacc gtggcgcctc tgtttgccca tggcgtcatg gtcatgccgg
     6541 acggcagcga gctgaacggt ccggaagagg tgctgcggac gttttccgac gtcatcatct
     6601 tctacgatga cgaggagaac gttgttccgt atcagcgcgg cgcctgctcg ccgcgaacca
     6661 agcacgtggt caccaacagc atcttcgagt tcgacaacgc ggttcgcaac gccgacgtgc
     6721 gcagctactt caccgtctat cagtctctgg gcggggccaa cgagatcatc gtcggcgggc
     6781 gctacatcga tcagtacgag ctgatgatcc agggctgggt gctcgtcaga cgcgaggtgt
     6841 acctggaaaa cgtcggagat ctgtcccgcc acctcaacgg ctgacccgat cagtcaggca
     6901 agccactcct cgggaatgtt gtcctccacg agctgccgga tgctgatgtt cctgatcccg
     6961 tgctcgctgc ccacctcgac cgcgagctcg gacttgtcgt tcgtgcgggc aaacacgaac
     7021 gcgttcggat agctctgctt gagccacagc gccgtgcgca gattgtcctc ggcccggccg
     7081 gtgccgagga tcaccgtcgg cgagtccttg gagagatcga cggaggcggc cagggaccgc
     7141 cagacctccg ggtgggagat gtccccctgc aataccaccc gtcggtagtt gccgcccagc
     7201 cgacgctgct cttcggcgac catc
//
LOCUS       DBNHOX010000346         6825 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1377121, whole genome shotgun sequence.
ACCESSION   DBNHOX010000346 DBNHOX010000000
VERSION     DBNHOX010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1377121"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="AC16UZ_07590"
     CDS             complement(<1..269)
                     /locus_tag="AC16UZ_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246585.1"
                     /translation="MPAVRDYALPLLLALVLHALAFLSLRAGWNPETAEVRAIKPQIV
                     RSTLVMMEPKAKPVPRPKAAAKASPLPARQLEEPKPKAEARPADPP"
     gene            complement(276..713)
                     /gene="tolR"
                     /locus_tag="AC16UZ_07595"
     CDS             complement(276..713)
                     /gene="tolR"
                     /locus_tag="AC16UZ_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HIR6246586.1"
                     /translation="MAKRRKPMSEINVVPYIDVMLVLLVIFMATAPLLTQGVIVDLPK
                     APSEPITDRQDDPLVVSIREDGAMFVNLGMQNADDESTRVTIYSLEDQAGKILRARPE
                     VPVYVKADHKLDYGAVVRVMTVLQRVGAQSVGLITDPPELAGE"
     gene            complement(724..1407)
                     /gene="tolQ"
                     /locus_tag="AC16UZ_07600"
     CDS             complement(724..1407)
                     /gene="tolQ"
                     /locus_tag="AC16UZ_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HIR6246587.1"
                     /translation="MPEQLSIFQLIANASLLVQVVMLLLALASVASWVMIFQRWFFLR
                     RVLASIADFEEHFWSGIDLRALYNELEAQDDLTGIESIFVAGFKEYTRLSEQSGADAD
                     SIMQGAQRAMRVALNREEENLETHLAFLATVGSTSPYVGLFGTVWGIMNSFRSLASMS
                     QATLASVAPGISEALVATAMGLFAAIPAVIGYNRFSARVEVMMSRYEAFSDELTGILH
                     RAAHARGGR"
     gene            complement(1419..2468)
                     /gene="ruvB"
                     /locus_tag="AC16UZ_07605"
     CDS             complement(1419..2468)
                     /gene="ruvB"
                     /locus_tag="AC16UZ_07605"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR6246588.1"
                     /translation="MSVEPDRLVSPREDGREGGFDRALRPERLDDYVGQCAVREQMEI
                     FIQAARARDEALDHTLIFGPPGLGKTTLANIIANEMGVAIKSTSGPVLERPAELAAML
                     TNLEEGDVLFVDEIHRLSPVVEEILYPALEDFQLDILIGEGPAARSLKLELPRFTLVG
                     ATTRAGLLTSPLRDRFGIVQRLEFYTVEELTQIVSRSAAKLGLEIHPEGATEVARRSR
                     GTPRIANRLLRRVRDWAEVKADGVINRKIADAALNMLKVDAQGFDSMDRRLLSAIIEK
                     FSGGPVGLDSLAAAISEERDTIEDVLEPFLIQSGYLMRTPRGRVATEQAYLHFGLSVP
                     EASEPSGGQPRLDLR"
     gene            complement(2465..3073)
                     /gene="ruvA"
                     /locus_tag="AC16UZ_07610"
     CDS             complement(2465..3073)
                     /gene="ruvA"
                     /locus_tag="AC16UZ_07610"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR6246589.1"
                     /translation="MIGRIQGQLVEVAENVILVNAGGICYEVELTSTALGRLPGADAP
                     VDLFTHFLVREDAQLLYGFASRDERDLFRALIRITGVGPKLALALISSIPLADLARAV
                     QTNDVALLTRVPGVGRKTAERLLVELKDRLADIVVVAEAGIPARGGKSAEEAEEVLVA
                     LGYKPQEAQRVVSDVLAEGSASTEEIVRAALRHIAMRKEVSG"
     gene            complement(3070..3543)
                     /gene="ruvC"
                     /locus_tag="AC16UZ_07615"
     CDS             complement(3070..3543)
                     /gene="ruvC"
                     /locus_tag="AC16UZ_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR6246590.1"
                     /translation="MALRILGIDPGSRITGYGLVEVSGGRIRYLASGCVRPRAKAFPE
                     RLAEIYTGIRSVISEHGPEVLAIEQVFMARNPDSALKLGQARGVAIAAAVQCGLPVHE
                     YAARTVKQAVVGSGRAGKEQVQHMIRAILDLPGAPAPDAADALAIAICHVNTSRP"
     gene            complement(3551..4291)
                     /locus_tag="AC16UZ_07620"
     CDS             complement(3551..4291)
                     /locus_tag="AC16UZ_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6246591.1"
                     /translation="MAGHSKWANIKHRKAAQDVKRGKLYTKIIREITVAARLGGGEVA
                     DNPRLRAAMDKALGANMPKDTIERAIARGAGGQDGSDVEELVYEGYGPAGVAILVEAM
                     TDNRNRTVAEVRHAISKHGGNLGTDGSVAYLFNRQGVISFAPGADEEQIMEVVLEAGA
                     EDIETEGDGSVSVYTPWESLSEVVGALKAEQLEPDNAEVTMVASTYSACEPEDAEKVL
                     QLIDALEELDDVQNVYSNGDFPDSVLGA"
     gene            complement(4311..6068)
                     /gene="aspS"
                     /locus_tag="AC16UZ_07625"
     CDS             complement(4311..6068)
                     /gene="aspS"
                     /locus_tag="AC16UZ_07625"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR6246592.1"
                     /translation="MRSHYCGELNAGNVGETVELYGWVNRRRDHGGVIFLDVRDRTGI
                     AQVVFDPDAPESFAVADSVRNEYVLRLTGRVRPRPEGTVNAEMATGEVEVLGAELEVL
                     NVSETPPFQLDEHSEAGEDVRLKYRYLDLRRPEMQSRIFARARIASAVRRFLEDEGYW
                     EVETPTLTRATPEGARDYLVPSRTHPGEFFALPQSPQVFKQLLMISGMDRYFQIARCY
                     RDEDLRHDRQPEFTQIDIEASFVTAEDVMALTERMLKQVFSQALDVELPEFPVLTWAE
                     AMSRYGSDKPDLRNPLELVDVADLMRDVEFKVFNAPANDPGGRVVALRAPGGGSLSRK
                     VIDDYADFVARYGAKGLAYIKVNDLGAGRAGLQSPILKFLPDEVVSEVLARVGAGDND
                     VIFFGADTAGVVADAMGALRDALAGDLDLLEDRWAPCWVINWPMFEMGRDGVPTPHHH
                     PFTRPTCTPEEMLAEPMVAEAAAYDVVLNGYELGGGSLRIHDREMQQAVFDVLRLGEE
                     AEVRFGFLLDALKFGCPPHGGIALGLDRLVMLMTGSHAIRDVIAFPKTQTASCPMTDA
                     PGPVDEHQLRELHIRVRTN"
     gene            complement(6262..6474)
                     /locus_tag="AC16UZ_07630"
                     /pseudo
     CDS             complement(6262..6474)
                     /locus_tag="AC16UZ_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017139808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
BASE COUNT         1177 a         2283 c         2145 g         1220 t
ORIGIN      
        1 ggggggtccg caggtcgcgc ttccgccttg ggcttcggct cttcgagctg ccgggcaggc
       61 aggggtgagg ccttggcggc ggctttcggt ctcgggacgg gttttgcctt gggctccatc
      121 atgaccagcg tcgaccgcac gatctgcggc ttgatggcgc gcacctcggc ggtctcgggg
      181 ttccagccgg cccggagaga gagaaaggcc agagcgtgca gtaccagcgc cagcagcagc
      241 ggcagcgcgt agtcacggac ggccggcatt gctcgtcact ccccggcaag ctcgggcggg
      301 tcggtaatca gaccgacgct ctgcgcgccc acccgctgca ggacggtcat cacccggacc
      361 acggctccgt agtccagctt gtgatcggcc ttcacgtaga cggggacctc gggacgcgca
      421 cgcaggatct tgccggcctg gtcctccagc gagtagatcg tcacccgggt gctctcgtcg
      481 tccgcattct gcatgccgag gttgacgaac atggcgccgt cctcacggat ggagaccact
      541 aggggatcat cctgtcggtc cgtgatgggc tcggaaggtg ctttcggcag gtcgacgatg
      601 acgccctgcg tcagcagagg cgcggtggcc atgaagatca cgagcagcac gagcatcacg
      661 tcgatgtacg gcacgacgtt gatctccgac atgggcttcc tgcgcttggc catgaacctg
      721 ccttcacctc cccccgcggg catgcgccgc gcggtggagg atgccggtca actcgtcgct
      781 gaacgcctcg taacggctca tcatgacttc cacccgggcg gaaaaccggt tgtaaccgat
      841 caccgccggt atcgccgcga agagccccat ggccgtcgct accagagctt ccgaaatgcc
      901 cggcgccacc gaggcgagcg tcgcctgact catgctggcc aggcttcgga aggaattcat
      961 gatgccccac acggtcccga acaggccgac gtacgggctc gtcgatccca ccgtagcgag
     1021 aaaggccaga tgggtttcga ggttttcctc ttcccggttc aaggccaccc gcatggcccg
     1081 ctgggctccc tgcatgatgg aatccgcatc cgcaccggac tgctccgaca gccgggtgta
     1141 ctccttgaag ccggccacga agatggattc gatacccgtg aggtcgtctt gggcttccag
     1201 ctcgttgtac aacgcccgca ggtcgatccc cgaccagaag tgctcctcga agtcggcgat
     1261 ggaagccagc acccggcgca ggaagaacca tctctgaaaa atcatgaccc acgaggcgac
     1321 ggacgcgagc gcgagcagca gcatgaccac ctgaaccagc aggctggcgt tggcgatcag
     1381 ctgaaatatg gacaactgct caggcacgat cgatcaactc acctcagatc gaggcgcggc
     1441 tggccaccgg agggctccga ggcttccggc acgctcaggc cgaagtgcag gtacgcctgc
     1501 tccgtggcca ccctgccgcg gggggttcgc atgaggtaac cgctctggat cagaaacggc
     1561 tcgaggacat cctcgatggt atcgcgctct tcgctgatgg cggccgccag ggagtcgagg
     1621 cccaccggcc cgccggagaa tttctcgatg atggccgaga gcaaccgtcg gtccatgctg
     1681 tcgaatccct gagcgtccac tttcaacatg ttcagagccg cgtccgcgat cttccggttg
     1741 atcaccccgt ccgccttgac ctccgcccag tcgcgcaccc ggcggagcag ccggttcgcg
     1801 atgcggggcg ttccacgaga tcttcgcgcc acctcggtcg cgccctccgg atgaatctcc
     1861 aacccgagct tcgccgcgga tcgcgaaacg atctgcgtga gttcttcgac cgtgtaaaac
     1921 tcgagtcgtt gcacgatgcc gaagcgatct cgaagcggcg acgtcagcag accggcgcgg
     1981 gtggtcgcgc cgaccagcgt gaatcgcggc agctcgagct tgagcgaccg ggccgccggg
     2041 ccttcgccga tgaggatgtc gagctgaaaa tcctccagcg cgggataaag gatttcctcc
     2101 accaccgggc tgaggcggtg aatctcgtcg acgaagagca cgtctccctc ctcgagattg
     2161 gtcagcatgg cggcaagctc tgccggacgc tcgagcaccg gcccggaggt ggacttgatg
     2221 gccacgccca tctcgttggc gatgatgttt gccaacgtgg tcttgccgag gccgggcggc
     2281 ccgaagatga gcgtgtgatc cagcgcttcg tctcgggcgc gggctgcttg gatgaagatc
     2341 tccatctgct ccctgaccgc gcactgcccg acgtagtcgt cgagccgctc gggccgcagc
     2401 gcacgatcga acccgccctc acggccatct tcccgggggc tgaccaaccg atccggctcg
     2461 acgctcaccc gctcacctcc ttgcgcatgg caatgtgcct cagggcggca cgcacgatct
     2521 cttccgtgct ggcggaccct tcggccagca cgtcgctcac gacgcgctgc gcctcctggg
     2581 gtttgtagcc cagcgccacc aggacctcct cggcttcctc agccgacttg cctcctcggg
     2641 ccgggattcc agcctcagcg accacgacga tgtcggcgag ccggtccttg agttccacca
     2701 gaagccgctc ggccgttttc ctgcccacgc cgggaacccg ggtcagcagg gcgacgtcat
     2761 tggtctgcac ggcgcgcgcc agatcggcca gcggaatgct cgatatcagg gccaacgcca
     2821 gcttggggcc cacgccggta atccggatca gggccctgaa gaggtcacgc tcgtcccggc
     2881 tggcaaagcc gtagagcagc tgagcgtcct cgcgaaccag aaagtgcgtg aagaggtcga
     2941 cgggggcgtc cgccccgggc aaccggccca gcgcggtgct ggtcaactcc acctcgtagc
     3001 agataccacc ggcgttgacg aggatcacgt tttcggcgac ctcgacgagc tgaccttgga
     3061 ttcgcccgat catgggcggg aagtattgac gtgacaaatg gcaatcgcaa gcgcatccgc
     3121 cgcgtcgggt gccggcgccc cgggaaggtc gaggatcgcc ctgatcatgt gctggacctg
     3181 ctcctttccc gcgcgccccg agccgaccac cgcctgcttg accgtgcggg ccgcgtactc
     3241 gtggacgggc aacccgcatt ggacggcggc ggctatcgcc acgccgcggg cctgtcccag
     3301 cttcagcgcc gagtcgggat tccgggccat gaatacctgc tcgatggcca gcacctcggg
     3361 gccgtgctcc gagatcacgc tccggatgcc ggtgtagatc tccgccagac gctcgggaaa
     3421 cgctttcgcg cggggccgga cgcagccgct ggcgagatag cggatccggc cgccggacac
     3481 ctcgaccaag ccgtagccgg taatgcggga acccggatcg ataccaagga tccggagcgc
     3541 catcctcgcc tcaggcgccc agcacgctgt ccggaaagtc gccgttgctg tagacgttct
     3601 gcacgtcgtc cagctcttcc agcgcgtcga tgagctgcag caccttctcg gcgtcctccg
     3661 gctcgcacgc gctgtaggtc gaggccacca tcgtcacctc ggcgttgtcc ggctcgagct
     3721 gctcggcctt gagcgctccg accacctcgc tcagcgactc ccagggggtg tagacggaaa
     3781 ccgagccgtc tccctcggtc tctatgtcct cggcaccggc ctcgagcacc acctccatga
     3841 tctgctcctc gtcagcgccc ggggcgaagc tgatgacgcc ctgacgattg aagagatacg
     3901 ccacggaccc gtccgtgccc aggttccctc cgtgcttgct gatcgcatgt cggacctccg
     3961 caaccgtgcg gttgcggttg tcggtcatgg cctcgacgag aatcgcgacg cccgctgggc
     4021 cgtacccttc atagacgagc tcttcgacgt cgctgccatc ctgccccccg gcgccccggg
     4081 cgatggcgcg ctctatggtg tccttcggca tgtttgcgcc cagggctttg tccatggcgg
     4141 cgcgcaggcg cgggttgtcg gccacctcgc cgcccccgag ccgggcggct accgtgatct
     4201 cacgaatgat cttcgtgtaa agcttgccgc gcttgacatc ctgcgcggct ttcctgtgct
     4261 tgatgttcgc ccacttgctg tgaccggcca tgcccgcctc cgattgatcc tcagttcgtg
     4321 cgcacgcgga tgtggagctc tcgcaactga tgctcgtcta caggtccggg cgcatcggtc
     4381 atgggacacg acgccgtctg ggtcttggga aacgcgatca cgtcgcggat cgcgtgcgaa
     4441 ccggtcatga gcatcaccag acgatcgagc ccgagcgcga tgcctccgtg aggcggacac
     4501 ccgaacttca gcgcatcgag cagaaaaccg aagcgcacct ccgcttcctc gccgagcctg
     4561 agtacgtcga agacggcctg ctgcatttct cgatcatgaa tccggaggga gcccccgccc
     4621 agctcgtagc cgttgagcac cacgtcgtat gcggcagctt cggcgaccat gggctcggcc
     4681 agcatctcct cgggggtgca cgtaggccgg gtgaaaggat ggtggtgcgg cgtcgggacg
     4741 ccgtcgcgcc ccatctcgaa catgggccag ttgatcaccc agcacggcgc ccatctgtcc
     4801 tccagaaggt cgagatcgcc ggccagcgcg tcgcgcagcg cgcccatggc gtcggcaacc
     4861 acgccggcgg tgtcggcgcc gaagaagatc acatcgttgt caccggctcc gacgcgcgcg
     4921 agcacctcgg acaccacctc gtcggggagg aacttgagga tcggcgactg caggccggcc
     4981 ctgccggctc cgaggtcgtt caccttgatg taggcgagcc ccttcgcgcc gtagcgcgcg
     5041 acgaaatcgg catagtcgtc gatgaccttg cggctgagcg agcctccgcc gggggcccgt
     5101 agcgccacca cacgaccgcc agggtcgttg gccggagcgt tgaagacctt gaactcgacg
     5161 tcgcgcatca ggtcggcgac gtcgacgagt tccagcgggt ttctcaggtc cggcttgtcg
     5221 ctgccgtaac ggctcatggc ttcggcccag gtgagcacgg gaaactcggg cagctcgacg
     5281 tcgagcgcct ggctgaacac ctgcttgagc atgcgctcgg tcaatgccat gacgtcctcg
     5341 gccgtgacga acgaggcctc gatgtcgatc tgcgtgaact cgggctgcct gtcgtggcga
     5401 agatcctcgt cccggtaaca gcgggcgatc tggaagtagc gatccatacc cgagatcatg
     5461 agcagctgct tgaacacctg cggcgactgc ggaagcgcga agaactcgcc cgggtgggta
     5521 cggctgggaa ccagataatc gcgcgcgccc tcgggcgtgg cgcgcgtcag cgtcggggtt
     5581 tcgacttccc agtacccttc gtcctccagg aagcggcgga ccgcgctggc aatgcgtgcc
     5641 cgcgcgaaaa tgcggctctg catttccggg cggcgcaggt cgaggtagcg gtacttgaga
     5701 cggacgtcct cgcccgcttc cgaatgctcg tcgagctgaa acggcggggt ctcggagacg
     5761 ttgagcactt cgagttccgc tccgaggacc tccacctcgc cggtggccat ctcggcattc
     5821 accgtgccct cgggtcgcgg ccggacccgc ccggtcagcc ggagaacgta ctcgttgcga
     5881 acgctgtcgg ccactgcgaa gctctccggg gcatccgggt cgaacaccac ctgggcaatc
     5941 cccgtccggt cacgaacatc gagaaagatc acgccgccat gatcacggcg gcgattcacc
     6001 cagccgtaaa gctctaccgt ctcgccaacg ttgccggcgt tgagttcccc gcagtagtga
     6061 ctacgcatgg ctgcttcctg ctcgcatgaa tccagagcgt cccttggttc cgatgggttg
     6121 tgctaagact tattccgatt tgctcgtggt cgagcccgag gtggtcgagc tgccgggctt
     6181 ggcaccgcca tcggccggct tgctcgaact cgctgacgac gactccgaag tggcgcccgc
     6241 gctcgacttg tccgcgcttt cctttttgtc ttcggtgagg ttgcgcttgt ttccggactt
     6301 gaaatcggtc tcgtaccagc cgccaccctt caaccggaat ccgggcgccg acaccttctt
     6361 gaacagagcg tcctgcgcac actcgggaca cgtctgcaaa ggcgcttcgc ttatcttctg
     6421 gatgacttcc agctcgtggc cgcagttcga gcagcgatat tcgtagatgg gcatgatcga
     6481 aaatctctga ttctgcaaac gtcagttgag cgagcggcgc atcatcttaa aaaaaccagc
     6541 aaaaatacag cgatatcgca ggctttttgt tgcttggccg cttccgatcg gctataaacc
     6601 gcggccatcg agagtgtcgg agtgacggcg agctagccag gaatatcagg cgtttccgtg
     6661 caagccgcga tctcagccgc cgcagtcttt cggtaaatgg ggacccgcta cgcaaccggg
     6721 tatcggagag cgaagctggt tcagctaaat aatccagctc aaggaatatt gaatggcacg
     6781 caaaaaggcc cccgccaaga aaaaggctcc ggcaaagaag aaggc
//
LOCUS       DBNHOX010000347         3605 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_606408, whole genome shotgun sequence.
ACCESSION   DBNHOX010000347 DBNHOX010000000
VERSION     DBNHOX010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_606408"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="AC16UZ_07635"
     CDS             complement(<1..266)
                     /locus_tag="AC16UZ_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246593.1"
                     /translation="MLDRTDRRRFVRQLGLLGVAGQTLLRSGSLRAAVGEARDSAALD
                     SAWPEMAYRRLGRTGFEGSRLIFGCGAALMDRPRDDLLERAFEAG"
     gene            369..1148
                     /locus_tag="AC16UZ_07640"
     CDS             369..1148
                     /locus_tag="AC16UZ_07640"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246594.1"
                     /translation="MANILDQFKLDGQVAVVTGGGRGIGEGIALGMAEAGADIVVAAR
                     RTEEIEAVAEKVRALGRRALAVTTDMMQIEQVQALAQRAFEEMGKLTCWVSNAGGADD
                     RVPRTLLEMPERQWDFQLNLNLKAVWTGAQAAANIMKDHGGGTIINISSTAANRASPF
                     NGPYGVAKSGVNNLTQTLGAELAQFGIRVNGVSPGPIPTEVFMEFFGITEDDIPKLGE
                     RFEIPLGRVGTPEDIATAVIFLASRASSWMTGETFLVNGGR"
     gene            1198..2865
                     /locus_tag="AC16UZ_07645"
     CDS             1198..2865
                     /locus_tag="AC16UZ_07645"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6246595.1"
                     /translation="MSKADLVIRNATVVDGTGNPAVTGDVAVKDGRILAVGDFGGEAT
                     REIDASGLVLAPGFIDIHTHFDPQLCWDGLATPSIEHGITTVVIGNCSLSLAPVKGDG
                     RDKIVNMFGVIEDIKKPTFDAAVPFSWESFEEYLDFIRPNLGINVAALIGHSALRFYV
                     MGAQSQERAATADEIEEMCRLVEEAMAAGAAGISSSYVDIDENMNPVPSRLADLDEKI
                     ALAKAMAKSGRGIWQVVPYFPDMQQQLANIRELGEISLAAGVPCSIQPVLSSPTDPTA
                     SEILTALEAERERGARVYGQVMPRCFDMNMRLSETSMLLYALPKWKAIMDMPRSERRA
                     RFEDPAMRTELVEEMRNAQGMSMALPMLTVGRVQAEANQPYQGRMLAEIAQAEGKDLG
                     EVILDLSLADDLDAEFRLQNVLNADKAAVADLIDHPLLHFGASDAGAHITQFCGTGDT
                     THLLEHFVRDSGRLTLERGIHRMTGEVAADWGLADRGTIEAGKAADLVLFDLNRIHCR
                     DEEFVDDFPGEARRYIRRADGYTAVLVNGEVVLENGTYTEARSGSIV"
     gene            2956..>3605
                     /locus_tag="AC16UZ_07650"
     CDS             2956..>3605
                     /locus_tag="AC16UZ_07650"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004576843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6246596.1"
                     /translation="MQYTNLGPTGVRVSRLCLGMMTYGSKSWRDWVLDYDEAKPFVEQ
                     ALEAGINFFDTADVYSNGASEEVLGRALTELSVDREDVVIATKCFNGTHERRRNRWGL
                     SRKHILSACDASLRRLGTDYIDLYQIHRFDYRTPIEETIDALSHLVRVGKVRYLGASS
                     MFAWQMAKYLFTADAMGGVRFVTMQNHYNLIYREEEREMIPLCIDQGVGLIPWSPIA"
BASE COUNT          669 a         1204 c         1159 g          573 t
ORIGIN      
        1 cccgcctcga aggcgcgctc gagcaggtcg tcccgggggc ggtccatgag cgccgccccg
       61 catccgaata tgaggcggct cccctcgaag cccgtgcggc cgagcctgcg gtaggccatc
      121 tccggccagg ccgagtcgag cgcggccgaa tcgcgcgctt cgcccaccgc cgcgcgcaag
      181 ctccccgagc gcagcagcgt ctggccagcc acgccaagca gcccaagctg gcgaacgaat
      241 ctccgacggt cagtgcggtc gagcatgtca cggctcccct gccgattagc gatccgctca
      301 ttctaggcga tcgcccggac aatttgggta tactgctgcg ctttgacttt tcccgggggc
      361 aaggggcaat ggcgaacata ctcgaccagt tcaagctaga cggtcaggtt gcggtggtaa
      421 ccggcggcgg acgaggcatc ggcgaaggca tcgcgctggg catggcggaa gcgggtgcgg
      481 acatcgtggt cgcggcacgt cgcaccgagg agatcgaagc ggtggccgag aaggtcaggg
      541 ctcttggccg acgggctctg gcggtcacca cggacatgat gcagatcgag caggttcagg
      601 ctctggctca gcgtgcgttc gaggagatgg gcaagctgac ctgctgggtc agcaacgcgg
      661 gcggcgccga cgaccgggtg ccgcgcacgc ttttggagat gccggaacgg caatgggact
      721 ttcagctgaa cctgaacctc aaggcagtct ggaccggtgc ccaggccgca gcgaacatca
      781 tgaaggacca cggcggcggg acgatcatca acatctcgtc cacggccgcc aaccgcgcct
      841 cgcccttcaa cggcccctat ggggtcgcga agtcgggcgt gaacaatctc acccagaccc
      901 tcggcgcgga gctcgcgcag ttcggcatcc gcgtcaacgg ggtctcgcca ggccccatcc
      961 ccaccgaggt gttcatggag ttcttcggca tcaccgaaga cgacatcccg aaactgggcg
     1021 agcgtttcga gataccgctc ggccgcgtag gcacccctga agacatcgcc accgccgtga
     1081 tctttctggc gtcccgtgcc tcctcctgga tgacgggaga aactttcctg gtgaacggcg
     1141 gccgttgatc caaggagccc ggtcgactga gcgatcgcta atcatgagga aattcggatg
     1201 agcaaagctg atctggtcat acgcaacgct accgtcgtag acggcaccgg caacccggca
     1261 gtcaccgggg acgttgccgt gaaggacggc aggatactcg ccgttggcga cttcgggggc
     1321 gaggccaccc gcgagatcga cgcctccggc ctggttctgg ctcccgggtt catcgacatc
     1381 cacacccact tcgacccgca gctttgctgg gatggcctcg ccacgccatc catcgagcac
     1441 ggcatcacca ccgtggtcat cggcaactgc tcgttaagcc tcgcccccgt caagggcgac
     1501 ggccgcgaca agatcgtcaa catgttcggg gtgatcgagg acatcaagaa gccaacattc
     1561 gacgcggcgg tgccgttcag ctgggagagc ttcgaggagt acctggactt catccggccc
     1621 aatctcggca tcaacgtggc ggcgctcatc ggccactccg ccctgcgctt ctacgtgatg
     1681 ggcgcacaga gccaggagcg ggcggcaacc gccgacgaga tcgaggagat gtgccggctg
     1741 gtggaagagg ccatggccgc cggcgcggcc ggcatctcct cgtcttacgt ggacatcgat
     1801 gagaacatga acccggtccc cagccggctg gccgacctgg acgagaagat tgcgctggcc
     1861 aaggccatgg ccaagagtgg tcggggcatc tggcaggtgg tgccctactt ccccgacatg
     1921 cagcagcagc tggccaacat tcgagagctc ggcgagatca gcctggccgc cggcgtgccc
     1981 tgttccattc agcccgtgct ttcctcgccg acagacccca cggccagcga gatcctcacg
     2041 gcactggagg cggaacggga gcgcggcgcc cgcgtctacg gccaggtcat gccccgctgc
     2101 ttcgacatga acatgcgtct ttcagagacg agcatgctgc tgtatgccct gcccaagtgg
     2161 aaggccatca tggacatgcc tcgcagcgag cgccgcgcac gtttcgagga tcccgccatg
     2221 cgtaccgagc tggtggagga gatgcgcaac gcccaaggca tgagcatggc cttgccgatg
     2281 ctcaccgtgg gccgggtgca ggccgaggcc aaccagcctt atcaaggcag gatgctggca
     2341 gagatcgcgc aagccgaggg caaggatctc ggcgaggtga tcctggatct gtccctggca
     2401 gacgatctgg acgcggagtt ccgcttgcag aacgtgctca acgccgacaa ggcagccgtc
     2461 gccgacctca tcgaccatcc gctgctgcac ttcggcgcct ccgacgccgg cgcccacatc
     2521 acccagttct gcggtaccgg cgacacgacc catctcctgg aacacttcgt ccgggacagc
     2581 ggcaggctca ccctggagcg cggcattcac cgcatgacgg gagaggtggc cgccgactgg
     2641 ggccttgccg accgcggcac catcgaggca ggcaaggcgg ccgatctggt gctgttcgac
     2701 ctgaaccgca ttcactgccg ggacgaggag ttcgtggacg acttccccgg cgaggcgcgc
     2761 cgctacatcc gccgggcgga cggctacacg gcggtgctcg tcaacggcga ggtcgttctg
     2821 gagaacggca cctacaccga ggcccgcagc ggcagcatcg tctaggcgcg accgcgcacg
     2881 cgccggcccc acggccggcg ccctttaccg agcccgcgtc tctgcctaca gttgcagagt
     2941 caccgggaga gaacgatgca atacaccaat ctgggtccca cgggcgtgcg cgtgtcgcgc
     3001 ctctgcctcg gcatgatgac ctacggctcg aaatcctggc gcgactgggt gctcgactac
     3061 gacgaggcga agcctttcgt cgagcaggcc ctggaggccg gcatcaactt cttcgacacc
     3121 gccgacgtct actccaacgg cgcatccgag gaggtgctgg gccgcgccct tacggagttg
     3181 agcgtggacc gcgaggacgt cgtcatcgcc accaagtgct tcaacggcac ccacgagcgg
     3241 cgtcgcaacc ggtggggact ctcacgcaag cacattctct cggcctgcga tgcctcactg
     3301 agacgactgg gcaccgacta catcgacctg tatcagatac accgtttcga ctaccggacc
     3361 cccatcgagg agaccatcga cgccctctcc catctcgtgc gggtgggcaa ggtgcgctac
     3421 ctgggcgcat cgagcatgtt tgcctggcag atggccaagt acctcttcac cgcggacgcc
     3481 atgggcggcg tgcgcttcgt gaccatgcag aaccactaca acctgatcta cagagaggaa
     3541 gagcgggaga tgatcccgct gtgcatcgat cagggcgtcg gcctgatccc gtggagcccc
     3601 atcgc
//
LOCUS       DBNHOX010000348         3559 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1120604, whole genome shotgun sequence.
ACCESSION   DBNHOX010000348 DBNHOX010000000
VERSION     DBNHOX010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1120604"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..892)
                     /locus_tag="AC16UZ_07655"
     CDS             complement(47..892)
                     /locus_tag="AC16UZ_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018928949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6246597.1"
                     /translation="MRWLTFLRDGRATFGYVTSNGQGVVDVGARSETADLRSAIAAGA
                     LADLAGSCGDEADVPLDGLTYLPTIPNPDKILCVGLNYKAHQEETGRGGEGFPTIFVR
                     FATAQIGHEQPMIRPTESATLDYEGEIALVIGIPGRRISRSDALDHVAGVSIYNDGSV
                     REYQRQTSQFTAGKNFVGTGGFGPWMMTPDEVGDLNSMEITTRLNGEVMQNATADLLV
                     HGFAELIEYCSTFIELLPGDVIVTGTPGGVGVARNPPVFMDEGDVVEVEVKPVGVLRH
                     RVVVG"
     gene            complement(901..1983)
                     /locus_tag="AC16UZ_07660"
     CDS             complement(901..1983)
                     /locus_tag="AC16UZ_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HIR6246598.1"
                     /translation="MKAAVFREINQPMEIEEVALSKPGPREVLVRTAAAGVCHSDLHF
                     FNGTYPGLLPMVLGHESAGVVEAVGSDVHYVKPGDHVITCLSVFCGHCEFCLTGHLSL
                     CQEPETRRAPDQEPRISHDGAPVQQFANLGSFAEMMLVHEHAVAKIREDMPLDRAALI
                     GCGVTTGVGAVIHTAKVEPGSTVAVIGCGGVGLSCINGAAIAGASRIIAVDMVASKLD
                     LARKFGATDVVNAAEMDAINEVKALTGGGVHYSFEAIGLKATAEQAFGMLRNGGTATV
                     IGMIPPGDMVSLHGPDFLFEKKIQGSMMGSNRFRVDMPRFVDLYLQGKLHLDDLVSSR
                     IRLEDVNDAMAQLETGEIARNVIVFD"
     gene            complement(2055..3062)
                     /gene="pdxA"
                     /locus_tag="AC16UZ_07665"
     CDS             complement(2055..3062)
                     /gene="pdxA"
                     /locus_tag="AC16UZ_07665"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR6246599.1"
                     /translation="MTVGSDDDRPIAMTLGDPAGIGPEVAARLIAGGSLEGKLLLVGS
                     AWALGKGAEAAGVRLPDVEVVDDAGAIRGQVGLIDLGGEPESFRFGEVNAACGALAVR
                     AVEGAARLCLAGKVAAMVTCPINKAAVHAAGYTDDIGHQEILARLTGASWTATMLMTP
                     GLKVVHLSTHKSLIEAARFVTRRNVEEKLALLDETLGRWGLRNPRIAVAALNPHGGEQ
                     GLLGREELDEIEPAVAAARTAGIDAVGPLPADSVFNRAIDGEFDVVLAMYHDQGHIAI
                     KVHNFHESTTATLGIPFVRTSVDHGTAFDIAGSGRADERGLAAALDAARALSQGQLAR
                     L"
     gene            3136..3372
                     /locus_tag="AC16UZ_07670"
     CDS             3136..3372
                     /locus_tag="AC16UZ_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246600.1"
                     /translation="MLTLDDIARSFQVYAVCAPCQRTQPLPLAALIARFGGDLPVTAL
                     RTRLRCETCGQRSADMRIVYVGPCGSAAGFHYRR"
     gene            complement(3384..>3559)
                     /locus_tag="AC16UZ_07675"
     CDS             complement(3384..>3559)
                     /locus_tag="AC16UZ_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246601.1"
                     /translation="QSKEDERRAQWLEIGVGAQILRDLGLSRIRLIAGREVDYVGVEG
                     FGLVLESTELLND"
BASE COUNT          598 a         1255 c         1114 g          592 t
ORIGIN      
        1 aatcacggat ggaccgcggc cactcgccgg tgcggtggcg accggctcag cccacaacca
       61 cccgatgccg cagcacgcca acgggtttga cctccacctc gaccacgtcg ccctcgtcca
      121 tgaaaaccgg cgggttgcgg gcaacaccca ccccgcccgg cgtgccggtg acgatcacgt
      181 ccccgggcag aagctcgatg aaggtggagc agtactcgat gagctccgcg aacccgtgca
      241 cgagcagatc ggccgttgcg ttctgcatga cctccccgtt caggcgcgtg gtgatctcca
      301 tgctgttcag atcgccgacc tcgtccggtg tcatcatcca ggggccgaac ccgcccgtcc
      361 cgacaaagtt cttgcctgcg gtgaactggg acgtctgacg ctggtactcg cgcacgctgc
      421 catcattgta gatgctgacc cccgccacgt gatcgagggc atccgagcgg gatatgcgtc
      481 gaccgggtat tccgatcacg agggcgatct ccccttcgta gtccagcgtt gccgattcgg
      541 tcggacggat catcggctgc tcgtgcccga tctgggcggt cgcgaagcgc acgaagatcg
      601 tggggaatcc ttcgccgccc cgacccgtct cctcctgatg cgctttgtag ttcagcccaa
      661 cgcagagaat cttgtccggg ttcgggatgg tcggcaggta ggtcagcccg tcgaggggga
      721 cgtccgcctc gtcgccgcag gagccggcca gatcggccag ggcaccggcc gcgatggccg
      781 atctcagatc ggcggtttcg ctgcgggcgc cgacatcgac caccccctgg ccgttcgagg
      841 tcacgtagcc gaacgtggcg cgcccatcgc ggagaaaagt cagccagcgc atagttcccc
      901 ctagtcgaag acgatcacgt ttcgggcgat ctcgccggtc tcgagctggg ccatggcgtc
      961 gttgacgtcc tccagccgga tgcggctgga gaccagatcg tccagatgca gcttgccctg
     1021 caggtagaga tccacgaacc gcggcatgtc cacccggaac cggttggacc ccatcatgga
     1081 gccctggatc ttcttctcga acagaaaatc gggcccatgc agggagacca tgtcaccggg
     1141 aggaatcatc ccgatgacgg tggccgtccc gccgttgcgc agcatcccga atgcctgctc
     1201 ggccgttgcc ttcaggccga tcgcttcaaa ggagtagtgc acgccacccc cggtgagagc
     1261 tttcacctcg ttgatggcat ccatctccgc ggcgttgacc acgtcggtcg caccgaactt
     1321 gcgggccaga tccagcttgg aggccaccat gtccaccgcg atgatccggc tcgcgcccgc
     1381 gatggccgcg ccgttgatgc aggacaatcc caccccgccg cagccgatga cggccaccgt
     1441 ggagcccggc tccactttgg cggtgtgaat gacggccccg acaccggtgg tcaccccgca
     1501 gccgatgagc gcggcacggt ccagcggcat gtcctcgcgg atcttcgcca cggcgtgctc
     1561 gtggacgagc atcatctccg cgaacgagcc caggttggcg aactgctgca ccggcgcccc
     1621 gtcgtggctg atgcgcggtt cctgatccgg agcgcgccgg gtctcgggct cctggcacag
     1681 ggacagatga ccggtgaggc agaactcgca atgcccgcag aagaccgaaa ggcaggtaat
     1741 tacgtggtcg ccgggcttca cgtagtgaac gtccgagccc accgcctcca ccacaccggc
     1801 gctctcgtgg cccagcacca tcggcaagag gccgggatag gtcccgttga aaaagtgcag
     1861 gtcgctgtgg cagacgcccg ccgcggcggt gcgcacgagg acctcccgcg ggccgggttt
     1921 ggatagcgcc acctcctcga tctccatggg ctggttgatc tcgcgaaata ctgccgcctt
     1981 catgctgcct cccctgctgt gtgacgcgga cgcgacgccg cttcgattaa gcctcgcgag
     2041 tttgccccac ggccctagag ccgcgcaagc tgaccctggc tcaacgccct cgccgcatcc
     2101 agcgccgccg ccaggccgcg ctcgtcggcc cggcccgagc cggcaatgtc gaaggccgtt
     2161 ccgtgatcca ccgaggttcg cacgaagggg atgcccaggg tcgccgtggt gctctcgtga
     2221 aagttgtgca ccttgatcgc gatgtgaccc tgatcgtgat acatggccag gaccacgtcg
     2281 aactccccgt cgatggcccg gttgaacacg gaatccgccg gcagcggccc cacggcgtcg
     2341 ataccggcgg ttctggccgc cgccacggcg ggctcgatct cgtcgagctc ctcgcggccc
     2401 agcagaccct gctcgccgcc gtgagggttg agcgctgcca ccgcgatgcg cggattacgc
     2461 agcccccagc gccccagagt ctcatcgaga agggcgagct tctcctccac gttgcgccgg
     2521 gtaacgaagc gggcggcctc gatgagagat ttatgggtgg acagatgcac gaccttgagc
     2581 cccggcgtca tgagcatcgt ggccgtccac gatgcccccg tcagccgcgc cagaatctcc
     2641 tgatggccga tgtcgtccgt gtaccccgcc gcgtgcacgg ccgccttgtt gatcggacag
     2701 gtcaccatgg cggccacctt gccggccaga cagagccggg cggcgccttc gaccgcgcgc
     2761 acggcaaggg cgccgcaggc cgcgttgacc tctccgaagc ggaaggattc gggttcgccg
     2821 ccaagatcga tgagtccgac ctgaccccgg atggcgcccg cgtcgtccac gacctcgacg
     2881 tcggggagcc tcacgcccgc cgcttcggcg cccttgccaa gcgcccaggc gctcccgacc
     2941 aggaggagct ttccctccaa cgacccgccc gcaatcagcc tggcggcaac ctcgggaccg
     3001 atacccgccg ggtcgccgag ggtcatggcg attggccgat catcatcaga tcccacggtc
     3061 atggcatccc tccccgggga aaatactgta tatatataca gtactcatgg tcattagaac
     3121 gtcgacgccc ggaccgtgct cacgctagat gacatcgctc gcagtttcca ggtctacgcc
     3181 gtgtgcgcgc cgtgccagcg cacgcagccg ctgccgttgg cagcgctgat cgcccgattc
     3241 ggcggggacc tgccggtgac ggccctgcga acgcggcttc gatgtgagac gtgcgggcaa
     3301 cgaagcgcag acatgcgcat cgtgtacgtc ggcccctgcg ggtccgccgc cggcttccac
     3361 taccgccgct gacgagggga cggtcagtcg ttgagaagct cggtggattc gagaaccagg
     3421 ccgaaccctt ctacgcccac gtagtcgacc tccctgcccg cgatcagccg gatgcgcgac
     3481 aggcccagat cacgcaggat ctgcgcgccc acgccgatct ccagccactg agcccgacgc
     3541 tcgtcctcct tggattgat
//
LOCUS       DBNHOX010000349         4480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1569795, whole genome shotgun sequence.
ACCESSION   DBNHOX010000349 DBNHOX010000000
VERSION     DBNHOX010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1569795"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="AC16UZ_07680"
     CDS             <1..635
                     /locus_tag="AC16UZ_07680"
                     /inference="COORDINATES: protein motif:HMM:NF044751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic-ring hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6246602.1"
                     /translation="VHNLVWKLAYDLRDWSDAGLVDTYEAERKPVARFNADRAMDNSR
                     AVARIQRAVADGEAAAGEVARSRRYGNFRGLELGYSYRSAAVIDDGSPAPEPEDAVED
                     YVPCARPGHRMPHMWLEGGALSTLDLVQRRFLVLSDSARWLEAASQHNADCRVLPGNG
                     GFLQLFGLEPGGAVLVRPDGHVAARFAEPPGFPGGALASALRDLLNTTTT"
     gene            651..1523
                     /locus_tag="AC16UZ_07685"
     CDS             651..1523
                     /locus_tag="AC16UZ_07685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6246603.1"
                     /translation="MTDFGVLIFPTAYSISPSDLARAAEARGFESIFFPEHTHIPACR
                     TTPWPGGRELPREYWNTYDPYVGLAMAAAVTKTLKVGTGITLVTERDPIVLAKQVASL
                     DRLSGGRVLLGIGAGWNVEEMRHHGVAFDERWAVLRERVLAMRAIWTEQAPEFHGEHV
                     DFEPLWSEPKPLQAGGPKILMGASSRWTWARIAEYADGWFPIHQDPARASAQGAVDYP
                     QGIQATREAWQAAGRQGEPDFSIFGMGPDRGRTEEMIRLGFNRVIFGLPSADADTVLP
                     LLDRYAEIGHAVNS"
     gene            complement(1518..2138)
                     /locus_tag="AC16UZ_07690"
     CDS             complement(1518..2138)
                     /locus_tag="AC16UZ_07690"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6246604.1"
                     /translation="MKRALLLDLGNVVLRVDFRRTFDYWAEAARVPAERLYRRWQLDE
                     AYELHEVGALAFEEYIAALSRRLMIDLPMEEWRAGWNAVFAGAFQDVQARLPTVRQSM
                     PVYLFTNTNATHQAAWSHLYADTLSHFDALYVSSEIGHRKPNVSAFRHVAEDMGYAPE
                     QIVFVDDTKENVLGARAAGMDARMVGSEADVVRILDELTGTEPGDS"
     gene            2465..3529
                     /locus_tag="AC16UZ_07695"
     CDS             2465..3529
                     /locus_tag="AC16UZ_07695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6246605.1"
                     /translation="MELWTLTHSRADNVLAVARRAEAAGWAGVMVVDSQNLAADSYVG
                     LAVAATGTQRIGLGTGVTNTVTRHPAVTASAAMAVQDVSGGRMVLGIGRGDSALAHLG
                     RSPARLPGFERYLDVLQRYLKGEAVPFEDIPMDDHIAAPVADLDLADHPSASRIRWFK
                     DDVAKVPVEVAATGPKVIEMAARLADRVMFTLGAVEERLSWAMETAMAARTGAGIPDD
                     PAFGAYVNLVCHRDRAVARDLVRGGLTTFARFSVMHGQIAGPADDHDRATLTSLHDGY
                     DMKAHTRADSSQAALLDDDFVDRFAIAGPPDYCVERLNRLASMGIEKVAISGPTAGTD
                     PVEARRALALLDEAVVPQLA"
     gene            complement(3537..>4480)
                     /locus_tag="AC16UZ_07700"
     CDS             complement(3537..>4480)
                     /locus_tag="AC16UZ_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR6246606.1"
                     /translation="CLIYIGELCRYLMNTPAQPDDHDNPLVTMMGNGLRPDIWPAFKS
                     RFGIKRITEFYGASEGNVSFVNLLNKDCTIGMTGSKVALVRYDVDEDEIVRDENGHCM
                     EVAPGEPGLLLGHISEDAVFEGYTNAEATESKVVRNAFEDGDAWFNSGDLIRQVDVGY
                     SLGYPHYQFVDRVGDTFRWKSENVSTNEVGELINGFPDVKFCNVYGVEIPGADGRAGM
                     VAITLADGVTELDADALSRHVSDTLPPYARPVFVRVQPDIDVTGTFKMVKGDLRKQGY
                     DLDQITDPLFVMKPGESAYVPLDREFAGKIASGAAGY"
BASE COUNT          697 a         1446 c         1546 g          791 t
ORIGIN      
        1 acgtgcacaa cctcgtatgg aaactggcct acgacttgag ggactggtcg gacgccggtc
       61 tcgtcgacac ctacgaagcg gagcgaaagc cggtggcccg gttcaacgcg gatcgggcca
      121 tggacaactc gcgcgccgtg gcacgcatcc agcgggcggt cgccgacggt gaggccgcgg
      181 cgggcgaggt ggcgcggtcg cggcgctatg gcaatttccg gggtctggag ctggggtaca
      241 gctaccgatc ggcagcggtg atcgacgacg gcagcccggc gcccgagccg gaggatgccg
      301 tggaggacta cgtgccctgt gcccgccccg gtcaccgcat gccccacatg tggctggagg
      361 gaggcgccct gtccaccctg gacctggtgc aacggcgctt tctggtgctt tcggactcgg
      421 cgcgctggct ggaagcggcc tctcagcaca acgccgactg tcgggtgctc cccggcaacg
      481 gcggttttct ccagctgttc gggctcgagc cgggtggcgc ggtgctggtg cgcccggacg
      541 gccacgtggc ggcccgattc gccgagcctc ccggctttcc cggcggggcg ctggcgtcgg
      601 cgctcagaga tctgctcaac accaccacaa cctgataagg agattgccag atgacggact
      661 tcggcgtgct catcttcccc accgcctact ccatctcccc ttcggacctg gcgcgcgcgg
      721 ccgaggcccg cggtttcgag tcgatcttct ttccggagca tacccacata ccggcctgcc
      781 ggaccacgcc ctggccggga ggcagggagc tgccgcgcga gtactggaat acctacgacc
      841 cctatgtggg gctcgccatg gccgcggcgg tcacgaagac gttgaaggtg ggtacgggga
      901 tcacgctggt taccgaacgg gatcccatcg tgctcgccaa gcaggtggct tctctggatc
      961 gtctgtccgg gggccgggtg ctgctcggca tcggtgccgg ttggaacgtt gaggagatgc
     1021 gtcatcacgg ggtcgctttc gacgagcgtt gggccgtgct cagggagcgg gtgcttgcca
     1081 tgcgggccat atggacggag caggcgccgg agtttcatgg ggagcacgtc gacttcgagc
     1141 ctctgtggtc cgagccgaag ccgctgcagg cgggtggccc gaagatcctg atgggcgctt
     1201 cctcccggtg gacgtgggcg cggatcgcag agtacgcgga cggctggttc cccattcacc
     1261 aggatcccgc ccgggcgtcg gcccagggag ccgtggacta cccgcagggg atacaggcca
     1321 cccgcgaggc gtggcaggcg gccggccggc agggggagcc cgacttcagc atcttcggaa
     1381 tggggcctga ccgggggcgc accgaagaga tgatccggct gggtttcaac cgggtgatat
     1441 tcgggttgcc ctcggcggac gcggatacgg tgttgccgct gctcgaccgc tacgccgaga
     1501 tcgggcacgc cgtcaactca tgaatccccg ggctccgtgc cggtgagctc gtcgagaatg
     1561 cgcacgacgt cggcttcgct gccgaccatg cgcgcgtcca tgcccgcggc ccgggcgccg
     1621 agcacgttct ccttggtgtc gtctacgaag acgatctgct cgggcgcgta gcccatgtcc
     1681 tcggccacgt ggcggaacgc agagacgttc ggcttgcggt ggccgatctc cgacgagacg
     1741 tagagcgcgt cgaagtggct cagggtgtct gcataaagat ggctccaggc agcctggtgc
     1801 gtggcgttgg tgttggtgaa caggtacacg ggcatgctct gccgcacggt gggcagacgc
     1861 gcctggacgt cctgaaaagc gccggcgaag acggcgttcc agccggcgcg ccactcttcc
     1921 atgggcaggt cgatcatgag ccggcgactc aacgcggcga tgtactcttc gaaggcgagg
     1981 gctcccacct cgtgcagctc gtatgcctca tccagctgcc agcggcggta aaggcgctca
     2041 gcgggcacgc gggccgcttc ggcccagtag tcgaaggtac gccgaaagtc cacgcgcagg
     2101 acgacgttgc ccagatccag cagcagggcg cgcttcatcg cccggcccgg atggtcgccg
     2161 gtggtgggcg ccgccgtatt ttcgggtctt tctgcattca gttggcgttc agcgaatcgg
     2221 ctctaaggtc tgcctcaagg tctcggtcgt tgcctggcag gtccggcacg gaggtgcgca
     2281 gtcgtcagga cccgcatggt attcggccgg tgcccagttc gctacggcat gcccccagcg
     2341 ggcccatgct ccctcccttc cccctggctg taggcggacg gccccgggcg ccagcccggg
     2401 gtttcccttt ttccaggggt tgaaccatca tggtggagaa ccatcgacga tggatgcccc
     2461 gccaatggaa ctgtggaccc tgactcactc ccgtgcggac aacgtgctgg ccgtcgcccg
     2521 ccgcgcagag gcggccggtt gggccggcgt gatggtggtg gactcgcaga acctggcggc
     2581 cgactcctac gtgggcctgg ccgtggcagc caccgggaca cagcggatcg gcctgggaac
     2641 cggagtcacg aacacggtca cgcgacatcc cgccgtgacc gcctctgccg ccatggccgt
     2701 tcaggacgtc tccggcgggc gcatggttct gggcatcggg cgcggggact ccgcgctggc
     2761 gcatctgggc agatcgccgg ctagactgcc cgggttcgaa cgctatctgg acgtgctgca
     2821 gcgctacctg aagggcgaag ccgtgccgtt cgaagacatt cccatggacg accacatcgc
     2881 cgctccggtt gccgatctcg atcttgccga tcacccgagc gccagccgca tccggtggtt
     2941 caaggatgac gtcgccaagg tgccggtgga agttgcggcc acggggccca aggtgatcga
     3001 gatggctgcc cggctcgccg accgtgtgat gttcaccctt ggcgcggtgg aggagcgact
     3061 gagctgggca atggaaacgg ccatggcggc cagaacgggc gccggcattc ccgatgatcc
     3121 ggcattcggc gcgtacgtga atctggtttg ccatcgtgac cgcgcggttg cccgggacct
     3181 ggtccggggc gggctcacga ccttcgcacg attttccgtc atgcacggcc agattgccgg
     3241 tcccgccgat gatcatgacc gggcaacgtt gaccagcctg cacgacggct atgacatgaa
     3301 agcgcacacg cgggcggact cgagccaggc agctctgctg gacgacgact tcgtcgatcg
     3361 tttcgccatc gcgggccctc ccgattactg cgtcgagcgc ctgaaccggc tcgcgtcgat
     3421 gggcatcgag aaggtcgcga tcagcggccc caccgccggc accgatccgg ttgaggcgcg
     3481 ccgggcactg gcgctgctcg atgaagcggt tgtgcctcag ctcgcgtgaa cgcgcctcag
     3541 tagccggctg cgcctgaagc gatcttcccg gcgaattccc ggtccagggg cacgtaggca
     3601 ctttcacccg gcttcatgac gaacagcgga tcagtgatct ggtccaggtc gtaaccctgc
     3661 ttgcgcagat cgcccttgac cattttgaat gtgccggtga cgtcgatgtc cggctgcacc
     3721 ctgacgaaga cgggcctggc gtaggggggc agcgtgtcgc tcacgtgacg ggacagggcg
     3781 tccgcgtcga gttctgtcac gccgtccgcc agggtgatgg cgaccatgcc ggcacggccg
     3841 tccgcccccg gaatctcgac gccgtaaacg ttgcagaact tcacgtcggg gaaaccgttg
     3901 atcagctcgc ccacctcgtt ggtggagacg ttctcggact tccagcggaa ggtgtccccc
     3961 acgcggtcca cgaactggta gtggggatag cccaggctgt agcctacgtc tacctggcgg
     4021 atgagatcgc ccgagttgaa ccaggcgtcg ccgtcctcga aggcgttgcg aacgaccttg
     4081 ctttccgtcg cttccgcgtt ggtatagccc tcgaaaacgg cgtcctcact gatgtggccg
     4141 agcagcagcc cgggctcgcc cggcgccacc tccatgcagt ggccgttctc gtcccggacg
     4201 atctcgtcct cgtccacgtc gtagcggacg agcgcaacct tggatcccgt catgccgatg
     4261 gtgcagtcct tgttcagcag gttcacgaac gagacgttgc cctcgctggc gccgtagaac
     4321 tcggtgatgc gcttgatccc gaagcgggat ttgaatgcgg gccagatgtc gggccgcagg
     4381 ccgttgccca tcatggtgac cagcgggttg tcgtgatcgt cgggctgggc gggcgtgttc
     4441 attagatagc gacacagctc gccgatgtag atcaggcagc
//
LOCUS       DBNHOX010000350         3775 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_862371, whole genome shotgun sequence.
ACCESSION   DBNHOX010000350 DBNHOX010000000
VERSION     DBNHOX010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_862371"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..128
                     /locus_tag="AC16UZ_07705"
     CDS             15..128
                     /locus_tag="AC16UZ_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR6246607.1"
                     /translation="MKMEHRITAPRDGVVAKVHVNAGDQVENGQLLVTLAE"
     gene            144..932
                     /locus_tag="AC16UZ_07710"
     CDS             144..932
                     /locus_tag="AC16UZ_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6246608.1"
                     /translation="MAGTQATLYETRKGAAWITLNRPANRNALSAPLVNELYDHLLVA
                     NDDAAVRSIVITGTGPAFCAGADLKEPPGQAIDGGRAVPYADVLSAILDSPKPVIAAV
                     NGAAFAGGLGLIGAADIVVTAEDVQFSFSEVRIGVIPAVISVVCLPKLGVHHGMKLIL
                     TGERFSGRRAVEMGFAHRAVPADELVDAVQEEIDMINLGGPNAMVECKKLVRRVPRLT
                     VEDGFREAAEWSSRMFRSDEAAEGMAAFREKRKPAWAVGEGEDE"
     gene            925..2709
                     /locus_tag="AC16UZ_07715"
     CDS             925..2709
                     /locus_tag="AC16UZ_07715"
                     /inference="COORDINATES: protein motif:HMM:NF018938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HIR6246609.1"
                     /translation="MSDVIRIGNCSGFYGDRLEAAREMVEGGPIDVLTGDYLAELTMS
                     ILYSQIQSRGEDMGYVGTFLKQVRDVVAICIERGIKIVSNAGGLNPRSMAAEVDKILA
                     ELGLEATVAYIDGDDLLPDLERLQAEGEPFTNLDTGMALAETDEKALTANVYFGGWGI
                     KEALDRGADIVICPRVTDAAVVLGPAAWKFGWARNDYDALAGALAAGHIIECGAQCCG
                     GNYSFFEEVPSFRNVGYPIAEIEADGSFTITKHPGTGGLVSVGTVTAQLLYEISTPAY
                     FNPDVVAHFDTMTIEQTGEDRVRVSGCRGSSPPPTHKVCFNTLGPCKQSLEVLLTGLD
                     IEKKAEIYVDQVFHNLGGREQFDDVDVQLIRSDKEDPATNEEAHAALRITVTTRDPAK
                     LGRLLQAKVTELALAAIPGNTGRGTAGFNGSPVTVHWPALVDSRHVTERVHVGGATTD
                     VLPTQRLGLEEIYYQEVPVDVAPAPEGDRVTIPFGRLFGTRSGDKGGNANCGVWARTD
                     AAYGFLHRYLTVDQLKKLLPDMAGYEIERYDLPNLRAMNFYIRGVLGTGAASNHRIDK
                     QAKSLGEYLRAKLIEVPDALVAELARAE"
     gene            2762..3487
                     /locus_tag="AC16UZ_07720"
     CDS             2762..3487
                     /locus_tag="AC16UZ_07720"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246610.1"
                     /translation="MSRLRDKVCLITGGARGQGAAEARAFAREGAKVWIADVLEDEGS
                     KLAAETGGEFRVLDVTSESGWQALVDEIIAVDGRIDVLVNNAGIYRANRLIHTTLDEY
                     EQVMAVNATGVFLGLRAVGREMKKAQAGSIVNISSYAGMAAAAGSFAYGASKWAVRGM
                     TKTAAVELARSGVRVNSIHPGAIDTEMLSEAGDPAKLVRGTPLGRVADADEVASMALF
                     LASDESSYSTGAEFLVDGGLFAI"
     gene            complement(3499..>3775)
                     /locus_tag="AC16UZ_07725"
     CDS             complement(3499..>3775)
                     /locus_tag="AC16UZ_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6246611.1"
                     /translation="TAGDAGQLDLPPAGTAAALQSLVSESGAGSLTELAYRFCRHEPG
                     VHVVLTGTGKLDHLRANIAAIDNDPLPAPVSAGLEGLFGSVDCLSGE"
BASE COUNT          669 a         1182 c         1325 g          599 t
ORIGIN      
        1 tggtcctgga agcaatgaag atggaacatc gcatcacggc gccgcgggat ggggtcgtgg
       61 cgaaggttca cgttaacgcg ggcgatcagg tggaaaacgg gcagctgctc gtgaccctgg
      121 cggaataagg agggctgaac ccagtggccg gcacccaagc aacactttac gagacgcgca
      181 agggggcggc ctggatcacc ctgaaccggc ccgcgaaccg caacgcgctc tcggctcccc
      241 tggtgaacga gctctacgat caccttctgg tcgccaatga cgacgccgcc gtgcgttcga
      301 ttgtcattac cgggaccggc ccggccttct gtgctggagc ggatctgaag gagccgcccg
      361 gtcaggccat cgatggcggt cgcgcggtgc cgtatgcgga cgttctcagc gccatcctcg
      421 acagcccgaa gcccgtcatt gccgccgtga acggcgcggc gtttgccggg ggcctcggtc
      481 tcatcggggc cgccgatatc gtggtgaccg cggaagacgt tcagttcagc ttcagcgagg
      541 tccgcatcgg cgtcatcccg gcagtcatct ccgtggtctg cctgcccaag ctcggcgtgc
      601 accacgggat gaagctgata ctcaccggcg agcggttctc ggggcgccgc gcggtggaga
      661 tgggcttcgc ccatcgagcg gtcccggcag acgagctggt cgacgcggtg caggaagaga
      721 tcgacatgat caatctcggc ggtccgaacg cgatggtcga gtgcaagaag ctcgtgcgcc
      781 gcgtgccccg gctgaccgtg gaagacggtt tccgcgaggc cgccgagtgg agctcccgca
      841 tgttccgatc cgacgaggca gccgagggca tggcggcgtt tcgcgagaag cgcaagccgg
      901 cctgggccgt gggagaaggc gaagatgagt gacgtgatac gtatcggcaa ctgcagcggc
      961 ttctacggcg accggctgga agccgcccgt gaaatggtgg aaggcggacc catcgacgtg
     1021 ctcaccggcg actacctcgc tgagctgacc atgtcgatcc tctactcgca gattcagtcg
     1081 cgcggcgagg acatgggcta cgtcggcacc ttcctcaagc aggtgcggga cgtagtcgcc
     1141 atctgcatcg agcggggaat caagatcgtc agcaatgcgg gcggcttgaa tccgcgcagc
     1201 atggcggccg aggttgacaa gatcctcgcc gagctggggc tggaggccac cgtcgcgtac
     1261 atcgacggtg acgatctgct ccctgatctg gagcggcttc aggccgaagg cgagcccttc
     1321 accaacctcg acacgggcat ggcgctcgcg gaaaccgacg agaaagccct cacggccaac
     1381 gtgtatttcg gcggctgggg tatcaaggaa gctctcgatc ggggcgccga catcgtcatc
     1441 tgcccgcgcg tgacggacgc ggccgtggtc ctcggtcctg ccgcgtggaa attcggctgg
     1501 gcccgtaacg actacgacgc gctggccggc gcgctggccg ccggtcacat catcgagtgc
     1561 ggcgcccagt gctgcggcgg aaactactcg ttcttcgagg aggttcccag ctttcgaaac
     1621 gtcggctacc ccattgcgga gatcgaagcc gacggcagct tcacgatcac gaaacatccc
     1681 gggaccggcg gcctggtctc ggtgggcacg gtgacggctc agctgctcta cgagatctca
     1741 acgcccgcct acttcaatcc ggatgtggtg gcccacttcg acacgatgac catagagcag
     1801 acgggtgagg atcgcgttcg tgtcagcggg tgccggggca gcagcccgcc cccgacccac
     1861 aaggtctgct tcaacacgct gggtccttgc aagcagagcc tggaggtgct cctcaccggg
     1921 ctcgacatcg agaagaaggc cgaaatctac gtcgaccagg tatttcacaa ccttggcggg
     1981 cgcgagcagt tcgacgacgt ggacgttcag ctgatccgga gcgacaagga agatcccgct
     2041 accaacgagg aagcccacgc tgcgctcagg atcacggtga cgacgaggga cccggcgaag
     2101 ctcgggcgcc tgctgcaggc taaggtcacg gagcttgcgc ttgccgccat ccccggcaac
     2161 accgggcgcg gaacggcggg cttcaacggc tcgccggtaa ccgttcactg gccggccctc
     2221 gtcgacagcc gccacgtgac ggagcgcgtg cacgttggag gagcgaccac cgacgtcctg
     2281 cccacccagc gtctgggctt ggaggagatc tactatcagg aggtccccgt cgacgtcgcg
     2341 ccggcgcccg agggggacag ggtcacgata ccgttcggcc gcctgttcgg gacccgttcg
     2401 ggtgacaagg ggggtaacgc gaactgcggc gtctgggctc gcaccgatgc cgcgtacggc
     2461 tttctgcacc gttacctgac cgtcgaccag ctgaagaagc tgctgccgga catggccggc
     2521 tacgagatcg agcgctacga cctgccgaat ctgcgggcca tgaacttcta cattcgcggg
     2581 gtgctgggca ccggagccgc gtcgaatcac cggatcgaca agcaggctaa atccctgggc
     2641 gagtaccttc gtgccaagct catcgaggtg cccgatgcgc tggtggcgga gctggcccgg
     2701 gcggaatgag tcccgcaccg atgcacggcg gggccagatg ggaaaacgaa ggagaagcca
     2761 agtgagccga cttcgggaca aggtctgtct catcacgggc ggcgcccgcg gtcagggcgc
     2821 ggcggaagcg cgcgcgttcg cccgggaagg cgcgaaggtc tggatcgccg acgttctgga
     2881 ggacgaaggc tcgaagctgg cggccgagac cggcggcgag ttccgggtgc tcgacgtcac
     2941 ctcggagagc ggttggcagg ccttggttga cgagatcatc gccgtcgacg ggcgaatcga
     3001 cgtgctcgtc aacaatgccg ggatctaccg ggcgaaccgc ctcattcaca cgacgctcga
     3061 cgagtacgag caggtgatgg ccgtgaacgc gaccggtgtc tttctggggc tgcgagcggt
     3121 gggccgggag atgaagaagg cgcaggcggg atccatcgtc aacatctcct cgtacgccgg
     3181 catggcggcg gcagccggct cgttcgccta tggcgcgtcc aagtgggccg taaggggcat
     3241 gaccaagacc gccgccgtgg agctggcccg ctccggcgtg cgggtgaact ctatccatcc
     3301 cggagccatc gacacggaga tgctgagcga ggcgggagat ccggcgaagc tcgtgcgagg
     3361 cacgcccctc ggtcgggtcg ccgacgcgga cgaggttgcc agcatggcgc tgtttctcgc
     3421 cagtgacgag agctcctact cgacgggcgc ggagtttctc gtcgacggcg gtctgtttgc
     3481 catatagccg ggtccggctc actcgccgga caggcagtcg acggatccga agaggccttc
     3541 caggccggcg gagaccggcg cgggcagggg gtcgttgtcg atggcggcga tgttcgcccg
     3601 cagatggtcg agcttgcccg tgcccgtcag caccacgtgc acgccgggct cgtggcgaca
     3661 gaaacggtag gcgagttcgg tcagcgagcc ggcgccggat tcgctcacca ggctttggag
     3721 cgcggcggcg gtgccggccg ggggcagatc gagctgtccc gcgtccccgg cggtc
//
LOCUS       DBNHOX010000351         2094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1247876, whole genome shotgun sequence.
ACCESSION   DBNHOX010000351 DBNHOX010000000
VERSION     DBNHOX010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1247876"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="AC16UZ_07730"
     CDS             <1..784
                     /locus_tag="AC16UZ_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005085 - guanyl-nucleotide exchange
                     factor activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HIR6246612.1"
                     /translation="PMPVMGDRTYTDIAVGGDHACAIATGGAAYCWGHNERGQLGTGN
                     FVNSAAPVAVSGGLTFTSITTHQMVSCGITLDGSAHCWGNGSAGNLGSGSKSNSNVPV
                     EIRGGLRFSSLRLGLWTSCGVTSTGDGYCWGYNGSGELGIGSKSPEESLEPVMVAGGH
                     RWTSISPGVAVTCGVTTAGGGFCWGGNAWGERGTGPFPAADVTSPTPVVGDLAFDSID
                     ADWCTCGVAGNVAYCWGPGDMGCVGDGALVHRGVPTKVAGQP"
     gene            1310..>2094
                     /locus_tag="AC16UZ_07735"
     CDS             1310..>2094
                     /locus_tag="AC16UZ_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6246613.1"
                     /translation="MGDDSIASLIERLSSAAAGKAWAEFLDEYAPLIRHIVRRHEHEP
                     AAADECFAHVCGALSDDGFLRLRRFHCKGPASFRTWLMAVVANLCRDWHRRQRGRFRP
                     VRAVAKLPELDQHVYRLMFVHGVSRTECVATLVPRFPGLTDSVVAEISARVFDLLTPQ
                     QRWQLGARRHAPVRSGLRDGGGDDDPMGRVPTADPGPDEQAEATQQRQQLRDALAKLT
                     PEQRLLLRLRYEQGLTLAEVARLTRQPDPFRANRQVQAALEALA"
BASE COUNT          326 a          659 c          749 g          360 t
ORIGIN      
        1 accgatgcct gtcatgggag atcgaacgta caccgacatc gctgtggggg gcgatcacgc
       61 atgtgcgatc gcgacaggtg gcgccgccta ttgttggggc cacaatgagc gcggacagct
      121 tggcaccggc aatttcgtga actcagcagc acctgtagca gtgtcgggcg gcctgacttt
      181 cacgtcgatc acgacgcacc agatggtgag ttgcggaatc acgcttgacg gatccgcaca
      241 ttgctggggt aacggtagcg ccggcaatct gggcagtggc tcgaagtcca acagtaatgt
      301 gccggtcgag atcaggggcg ggctcaggtt ctcatcgctg cggcttgggc tctggacgtc
      361 ctgtggcgtg acgtccaccg gtgacgggta ttgctggggc tacaacggca gcggagaact
      421 cggcatcggc tccaagagtc ccgaggagtc cttggagcct gtcatggtcg cgggcgggca
      481 tagatggaca tccatcagcc caggagtcgc ggtgacgtgt ggcgtcacga cggcgggcgg
      541 gggtttctgc tggggtggca atgcctgggg cgaacgaggg accggtccgt ttccggccgc
      601 ggacgtcacg tcacccaccc cggtcgtcgg cgacctggca ttcgattcga tcgatgccga
      661 ctggtgtacc tgcggcgtcg ccggcaatgt cgcgtactgc tggggtccgg gcgacatggg
      721 ttgcgtcggg gacggggcgc tggtgcatcg cggcgttcca acaaaggtcg cgggccagcc
      781 atgaccggcg atcggtagcg tgccgcgtgc catcttccac gcaagtcaaa gcaggaggct
      841 gcggtctggc cgctctcggt tgggcgcctt gaggatccga atcagcttgt cttctcctgg
      901 ggacgcgccc cgagagggcc ggtggccgag gccgattctc gttcctgctg caaacctttc
      961 aagcgacccc ggcctcgcgc cggggtttct gctcctatgg ctaatgatgt ctgcttccgg
     1021 ccggcagcag actcctggcg ccgcacctgc cagccgggtc ggtcgggtct gctgacggtc
     1081 gaaggcagcc agcggccgtc cggccgccgg aggtcccgac atccggtcgg aggcctcgat
     1141 cagcggctga tggcctcgtt tcggtaaatc ccgcttgcag gccgccccaa gccggagtaa
     1201 gcgtcgatcc ataatcagca tttcggatgg tacccgggcc atcggtgcaa aaaggggggc
     1261 ggctgttgcc cgtccgtcca cggggaccgg gcgcggcggt catgccagga tgggcgacga
     1321 ctcgatcgca agtctgatcg aacggctgtc ttcagccgca gccgggaagg catgggctga
     1381 attcctcgac gagtacgcgc cgctgatccg ccacatcgtc cggcgccacg agcacgaacc
     1441 cgccgccgcc gacgagtgct tcgcccatgt ttgcggggcg ctcagcgatg acggctttct
     1501 caggctgcgc agattccact gcaaggggcc ggcgagcttc cggacctggc tcatggcggt
     1561 ggtcgcgaat ctgtgtcgcg actggcatcg ccggcaacgc ggacgtttcc gcccggtccg
     1621 cgcggtcgcg aagctgcccg agctggacca gcacgtttac cggctgatgt tcgtgcacgg
     1681 cgtttcccgt acggagtgcg tagcaacact ggtgccgcgg ttccccggcc tgacggattc
     1741 ggtcgtggcc gagatcagtg cccgggtgtt cgacctgctg acgccgcagc agcgctggca
     1801 gctgggcgcg agacgccacg ctccggtccg gtccgggctg cgtgacgggg gcggggacga
     1861 cgatccgatg gggcgggtgc cgacggcgga tccgggcccc gacgagcagg cggaagccac
     1921 gcagcagcga cagcagttgc gcgacgccct ggcgaagctg acgccggagc aacgcctgct
     1981 gctgcggctc cgctatgagc agggcctgac actggccgag gtagcccgcc tgacccgcca
     2041 gccggatcca ttcagggcca accggcaggt ccaggcggcg ctcgaggcgc tggc
//
LOCUS       DBNHOX010000352         2088 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_927916, whole genome shotgun sequence.
ACCESSION   DBNHOX010000352 DBNHOX010000000
VERSION     DBNHOX010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_927916"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="AC16UZ_07740"
     CDS             complement(<1..313)
                     /locus_tag="AC16UZ_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6246614.1"
                     /translation="MTYTRMNVSAENGVGRIELNQPDEYNRMPLAFWRELPLALRKLD
                     AGGDVRALVLSSTGKHFSAGMDLSVFTSSARRDLDRGRAGEAGRRHIDRMQNVFNGLE
                     TV"
     gene            629..>2088
                     /locus_tag="AC16UZ_07745"
     CDS             629..>2088
                     /locus_tag="AC16UZ_07745"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6246615.1"
                     /translation="MLTFADPLRRAVQIAAEDTAVIHDGARYSYAEFNRRCGQLVAVL
                     RELGIQRGDRVAILAGNCNQYLETYVGVPAGGMVVVPLNTRHAQPELEYALKDSGARV
                     LLTDRDPGALGGIVDHVISIPDAYESHLAHAEPAGLGEGVSEADLAGLFYTGGTTGLS
                     KGVMLTHRNLIANTFNWLACSPHYSSDRTLIMAPLFHAAGSNGVLGAIWNGGCQVTLG
                     AFDPASALDLIEAHAITETLGVPTMLAAIAEEQHARPRKTDSLRLIAHGGSPIATEVI
                     RRTMSAFPTASMMEVYGATELSPLATVLVGEDKLVDDPRARSCGQALIGNEIRILNPD
                     GQTVDTGEVGEVVVRGPNVMEGYWNKAEQTAAVLKDGGYWTGDLGYMDDRGYLFLVDR
                     SKDMIVSGGENVYCTEVEEILYQHPAVLEAAAFGVPDEKWGEAVHAVVVPRDEFADLN
                     PSEIIDFCREHIAGYKVPKAVDIRSEPLPKSGPGKV"
BASE COUNT          362 a          631 c          707 g          388 t
ORIGIN      
        1 ggaccgtctc caggccgttg aagacgttct gcatccggtc gatgtggcgc cgtcccgcct
       61 cgccggcacg accgcggtcc agatctcgcc gggcggagct cgtgaagacg gagagatcca
      121 tgccggcact gaaatgcttg ccagtggagg agagaacgag cgcccgaacg tcgcctccgg
      181 catcgagttt gcgcagcgcg agaggcagct ctctccagaa ggccagcggc atgcggttgt
      241 actcgtccgg ctggttgagc tcgattcggc cgaccccgtt ttcggccgag acgttcattc
      301 gagtgtacgt catgacagtc tcgttgcggt taaccaagca tgttaccgat tctgcccgcg
      361 ggcggacacc gtcggctgtt ggaagatggc attgccagcg ccctggaagc cgctgataat
      421 cggccccgtg tcgggcaaca gataaggagg tgatcagtgt atctatctgc aacctatgca
      481 gaggaggggc tggtctcctg atcggcttct cgagaacggg cccctcaacg gggcccgttt
      541 tttttggcct gccaggtcgg gcgtatttcc acggccggac ctttctggga gactccggcg
      601 atctgcggga aatgtttccg ggggaaagat gctgacattc gcggatccgt tgcgtagggc
      661 cgtccagatc gccgccgagg acacggcggt gatacacgat ggcgcgcgct acagctacgc
      721 agagttcaat cgccgctgtg gccagctcgt tgctgttctg cgcgagctcg gaattcagcg
      781 cggtgaccgg gtggcgatcc tggcgggcaa ctgcaatcag tatctggaaa cctacgtcgg
      841 ggtccccgcg ggcggcatgg ttgtggtgcc gctgaatacc cgtcatgccc agccggagct
      901 cgaatacgcg ctgaaggatt ccggtgcccg cgtcctgctg accgaccgcg atccgggggc
      961 gctcggcggc atcgtcgacc atgtgatcag catccccgat gcttacgagt cgcatctcgc
     1021 ccatgccgag ccggccgggc tcggagaggg cgtgagcgag gcggatctgg ccggtctgtt
     1081 ttacaccgga ggcacgacgg gactgtccaa gggggtgatg ctcacccatc ggaacctcat
     1141 cgccaacacg ttcaactggc tggcctgctc gccgcattac tcctcggatc gcaccctcat
     1201 catggcgccg ctgtttcacg cggcggggtc caacggcgtt ctcggggcaa tctggaacgg
     1261 gggatgccag gttacgctgg gtgccttcga tccggcgtcg gcgctggatc tgatagaagc
     1321 ccatgccatc acggaaaccc tcggggtgcc gaccatgctg gccgccatcg ccgaggagca
     1381 gcatgctcgt ccccgcaaaa cggactccct gaggctgatc gcccacggcg gttcgcccat
     1441 cgccaccgag gtgatccggc gcaccatgag cgcgttccct acggcgtcca tgatggaggt
     1501 gtacggcgcc acggagctct ctccgctggc gacggtgctg gtgggagagg ataagctggt
     1561 cgatgatccc cgggcgcgct cgtgcgggca ggccctgatc ggcaacgaga tccgcattct
     1621 caaccccgat gggcagacgg tggacacagg ggaggtcggc gaggtcgtcg ttcgcgggcc
     1681 caacgtcatg gaaggttact ggaacaaggc cgagcagacg gcggcggtgc tcaaggacgg
     1741 cggttactgg acgggcgacc tcggctacat ggacgatcgg ggctatctct ttctggtgga
     1801 ccgcagcaag gacatgatcg tctcaggggg cgagaacgtt tactgcacgg aggttgaaga
     1861 gatcctctac cagcatccgg cggtgctgga ggccgccgcc ttcggcgtgc cggacgagaa
     1921 gtggggcgaa gcggtgcacg cggtggtggt gccgcgcgat gagttcgctg acttgaaccc
     1981 ctccgagatc atcgatttct gtcgtgagca cattgccggg tacaaggtcc ccaaggccgt
     2041 cgacatccgc tcggagccgc tgccgaaatc aggccctgga aaggtttt
//
LOCUS       DBNHOX010000353         3225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_26683, whole genome shotgun sequence.
ACCESSION   DBNHOX010000353 DBNHOX010000000
VERSION     DBNHOX010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_26683"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="AC16UZ_07750"
     CDS             complement(<1..724)
                     /locus_tag="AC16UZ_07750"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6246616.1"
                     /translation="MSQATPVNLTARYPELDNGPVRADVYWQPEIFDQEIERIFRPTW
                     HCVGREEQITATGEFFVKELPTFEFSVIVARGDDGAVRAFLNACPHRGNQVELTACGR
                     RSAFTCKFHAWSYDLKGRLKSVPDEAGFPGLDKAAIGLTEFPCETWQGFVFVNLNENP
                     PVSLIDYLGEQGADLEGYPFDQGTARFQFAGEVETNWKFLVDSFCETYHVPFLHRRTI
                     NSTMAGPDNPHGRVVDVRLKGDH"
     gene            894..1715
                     /locus_tag="AC16UZ_07755"
     CDS             894..1715
                     /locus_tag="AC16UZ_07755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase family protein"
                     /protein_id="HIR6246617.1"
                     /translation="MLDDYLDRIGHSGPLNADLATLTALHRAHITAIPYENLEIQLGR
                     ENTLDEKDIFTKLVARRRGGWCYEMNGLLTRALAQIGFQTQRVSGAVHRDEQGSVVEG
                     NHLVGLVELDRIWVVDVGLADGPLEPFPLEPRQWKEGALEFGLERLDSDWWRFHNHAH
                     GLARTFDFTKENRELAHFESMCRFLQTDPASPFVAFALASRRRENGFDALRDTTWFHV
                     EDGRLEKRKIEDEAEYSEVLEQILGNDLGEEAGVLWHRAEERSAAREAAQSATQD"
     gene            complement(1790..>3225)
                     /locus_tag="AC16UZ_07760"
     CDS             complement(1790..>3225)
                     /locus_tag="AC16UZ_07760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6246618.1"
                     /translation="LKSGKQCNHRKDADMTGSSGGVDEAAWDELGSGVRGALLREGDA
                     GYDEARSVWNGMMDRHPAAVLQCLGVSDVMAGVNFARNHGLPLAVKGGGHNVAGKGVC
                     DDGLTLDMSLMRSVRVDPGTLRARAEAGALWRDFDHEAQTFGLTTTGGVVSSTGVAGL
                     TLGGGIGYLTRTHGLACDNLLAADVVTADGRLVQASENENPDLLWALRGGGGNFGVVT
                     SLEFQLHEVGPDVAAAIVFYPLAAGADVLHAYREYAEGAPDAVACYAMVVRGPADLPS
                     EYAGQPVLAIIACYSGAVAEGLRALDPVGSFATPIAATIEGTPYRAMQQMFDPGSPHG
                     VRYYWKSQYLSGLPDEFLETVVRFAREMPGAYTAIGIEPLGGAMERVDPAGTAYPHRR
                     FPFNLGIWSGWDEPSEDSGVISWTREFFEAVVPFGAGAYVNYLSDDEGGRVDEAYGDN
                     YARLRQVKAQWDPGNLFRVNQNIAPRG"
BASE COUNT          584 a         1081 c         1014 g          546 t
ORIGIN      
        1 ggtgatcgcc cttcaggcgc acgtccacga cgcggccgtg ggggttgtcc ggccccgcca
       61 tggtgctgtt gatggttcgc cggtgcagaa acgggacgtg gtaggtttcg cagaacgaat
      121 ccaccagaaa cttccagttg gtctccacct cgcccgcgaa ctgaaagcgt gccgtgccct
      181 ggtcaaacgg ataaccctcg agatcggcgc cctgctcgcc cagatagtcg atgaggctca
      241 cgggcggatt ctcgttgaga ttgacgaaga cgaagccctg ccaggtctcg cagggaaact
      301 cggtcagccc gatggccgcc ttgtccagac cgggaaagcc cgcctcgtcg gggacggact
      361 tgagccggcc tttcaggtcg tagctccacg cgtgaaactt gcaggtgaac gcgctgcggc
      421 gaccgcacgc cgtcagctcc acctggttgc cccggtgggg acaggcattg agaaacgccc
      481 ggacggcgcc gtcgtcgcct cgggcaacga tgaccgaaaa ctcgaacgtc ggcagttcct
      541 tgacgaagaa ctcccccgtc gcggtgatct gctcctccct gccgacgcag tgccaggtcg
      601 gcctgaagat acgctctatt tcctgatcga agatctcggg ctgccagtag acgtccgctc
      661 ggaccggccc gttatcgagt tccgggtaac gggccgtcag gttgaccggc gtggcttgcg
      721 acactatttc catctcccct cggcgacggc tcgatactag tacaatcgcc ctccggattg
      781 cagcgccggc ggtcagggca cccgttcggc tgcggcgccc ctttgagcgg aactggttcc
      841 aaccgggtcc gcatgctagc ttgactgtcc catcggcgct cccatcgaaa gacatgctag
      901 acgactatct cgaccggatc ggccactcag gcccgctgaa tgccgacctc gcgacgctta
      961 cggcccttca tcgtgcgcac atcactgcca ttccctacga gaatctggaa attcagctgg
     1021 gccgcgaaaa cacgctcgac gagaaagaca tcttcacgaa gctcgtggcg cgccgccgcg
     1081 gcggctggtg ctacgagatg aacggtctgc tgacacgagc gctagcccag atcggctttc
     1141 agactcagcg cgtcagcgga gccgtgcatc gcgacgagca gggcagcgtc gtggaaggaa
     1201 atcacctggt cggcctcgtc gagctggacc gcatctgggt agtggacgtg ggtctggcgg
     1261 acggcccgct ggaacccttt cctttagaac cgcgccagtg gaaggagggc gctctcgagt
     1321 tcggcctgga gcggctggat tcggactggt ggcgctttca caatcacgcc cacgggctcg
     1381 cccgcacctt cgacttcaca aaagaaaacc gtgagctggc ccacttcgaa agcatgtgcc
     1441 gcttcctcca gaccgatcca gcctccccgt tcgtggcgtt cgcgttggcg agccggcggc
     1501 gggagaatgg gttcgatgcc ttgagggaca cgacctggtt tcacgtggaa gacggtcgcc
     1561 tggagaagcg caagatcgag gacgaagcgg aatactccga ggtgctggag cagattctcg
     1621 gaaacgatct gggcgaggaa gcgggcgtgc tttggcatcg ggccgaggag cggtctgcgg
     1681 cgcgcgaggc agcccagagc gcgacgcagg attgatggcc gccgtccgcg accaggccag
     1741 gtccttagac cggggcctgg acgaaaatcc tgccgccgac tcgagctgat cagccccggg
     1801 gcgcgatgtt ctggttgacc cggaacaggt tgccggggtc ccattgcgcc ttcacctggc
     1861 gcaggcgcgc gtagttgtcc ccgtacgcct catcgaccct tccaccttca tcatcgctga
     1921 ggtagttgac gtaggcgccc gcaccgaaag gcaccaccgc ctcgaagaac tctctggtcc
     1981 agctgatgac gccagaatcc tctgacggct catcccagcc cgaccagatc cccagattga
     2041 atgggaaccg gcgatggggg taggcggtgc ccgccgggtc cacgcgctcc attgccccgc
     2101 cgaggggttc gatgccgatg gccgtgtaag cgccgggcat ctcccgagcg aaacgcacca
     2161 ccgtctcgag gaactcatca ggcaaccccg agagatactg ggacttccag taatagcgca
     2221 cgccgtgggg gctgccggga tcgaacatct gctgcatggc tctatagggc gtcccttcga
     2281 tcgtggcggc aatgggcgtg gcgaatgacc cgaccgggtc cagcgcgcga aggccttcag
     2341 ccaccgctcc ggagtagcac gcgatgatcg ccaacacggg ctgccctgca tattccgagg
     2401 gaagatcggc gggacctctg accaccatgg cgtagcaggc gaccgcatcc ggcgcccctt
     2461 cggcatactc ccgatacgcg tgcaggacgt ccgcaccagc ggctaacggg tagaagacga
     2521 tggccgccgc gacatcggga cctacctcgt gcagctgaaa ctccagcgag gtgaccacgc
     2581 cgaagttgcc cccgcccccg cgcagcgccc acagcaggtc cggattctcg ttctcgctcg
     2641 cctggacgag cctgccgtcg gccgtgacga cgtccgcggc gagcaggttg tcgcaggcca
     2701 gcccatgggt tcgcgtcagg taaccgatgc cgccacctaa ggtcagaccg gcgacgcccg
     2761 tgctggacac cacgccgccc gtggtggtga ggccgaacgt ctgcgcctcg tgatcgaagt
     2821 cgcgccacag cgcgcccgcc tcggcgcggg cgcggagcgt cccgggatcc actcgcaccg
     2881 agcgcatgag cgacatgtcc agggtcagac catcgtcgca gacgccttta ccggcaacgt
     2941 tgtgaccacc gcctttcact gccagcggca ggccatggtt tcgggcaaag ttcacgccgg
     3001 ccatgacgtc gctgacccca aggcactgca gaacggcggc cggatgccga tccatcatgc
     3061 cgttccagac gctgcgcgcc tcgtcgtagc ccgcatcccc ctcgcgaagc agggcaccgc
     3121 gcacgcccga cccaagctcg tcccaggccg cttcgtcgac tcctccgctg gaacccgtca
     3181 tatcagcatc ctttcgatgg ttgcactgct tgccggactt tagag
//
LOCUS       DBNHOX010000354         4074 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1377545, whole genome shotgun sequence.
ACCESSION   DBNHOX010000354 DBNHOX010000000
VERSION     DBNHOX010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1377545"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1523)
                     /gene="ahcY"
                     /locus_tag="AC16UZ_07765"
                     /pseudo
     CDS             complement(162..1523)
                     /gene="ahcY"
                     /locus_tag="AC16UZ_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003414325.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
     gene            complement(1584..2540)
                     /locus_tag="AC16UZ_07770"
     CDS             complement(1584..2540)
                     /locus_tag="AC16UZ_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HIR6246619.1"
                     /translation="MGQTAGQQANLDEVVEVAKAVGDHLRASILRVLSDGSYSVTELC
                     HLFQIPQPALSHHLKVLLTANLVAKRREGNNVYYRRCTEPDDGLRQRLFETVDGTPLG
                     AALAERAAEIHAERSQRSNNFFAENAHRFADQQALICEAQVYVPAIVELLDHAGVDSG
                     PIIEVGPGGGQLLATLAGSFDDLIGIDSSSTMLAQAAERLDGLPNVKLRQKDFTELPA
                     IRRYRAVIAAMVVHHLASPQRFFRHASRILRHGGLLIVAELDRHEQEWVTDACGDLWL
                     GFEPGELEQWAAQAGLAPVESQYLAQKNGFQIQIHGYARNGT"
     gene            2649..3809
                     /locus_tag="AC16UZ_07775"
     CDS             2649..3809
                     /locus_tag="AC16UZ_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6246620.1"
                     /translation="MSWSSPDLVSKLPDVQTTIFTVMSALAREEGALNLSQGFPDFDG
                     PPELLERVQYYLTHGHNQYAPMTGVAPLREATAGKIADLYGCQLDPEQEVTVTSGATE
                     ALFCAIAAVVNAGDEVIVFDPAYDSYAPVVKLQGGVTRHVPLHPPDYRVDWDRVADNL
                     SDRTRLIIINTPHNPTGTVWGEDDIEALRAVIDGRDLFLLGDEVYEHIIFDGRQHASL
                     CRYPDLFARSFVVSSFGKTYHTTGWKIGYCAAPAPLSAEFRRIHQFVTFTSITPVQLG
                     LADFMTRHPEHHLGLGAFYEAKRDLFCSLLEGSRFGLTKSAGTYFQLLDYGDYSNEAD
                     TDLAVRLTREAKIASIPLSVFYADDPGHRMLRFCFAKDDATLRAGAEILLGL"
     gene            3879..>4074
                     /locus_tag="AC16UZ_07780"
     CDS             3879..>4074
                     /locus_tag="AC16UZ_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246621.1"
                     /translation="MKRPLLALLVCCAFGCSESSNETPASTAAAGDGPAPAAHPGEET
                     YNRFCFSCHAAGIAGAPRTGD"
BASE COUNT          680 a         1302 c         1284 g          808 t
ORIGIN      
        1 cttgagccgg cacccgtcaa cgacttgatc tgctctcggg ggtcgtcgtt gtccagcgtg
       61 ccgagctgct tgtttcggaa gtaaccgccg acgccggcca gaacgatggg catgagcatc
      121 acgatcagca ggcaccaaag tggggtggtc atgtctgtct cctagtactt gtacgattcc
      181 ggtttgaatg gaccgctttc gtccacaccg atgtattttg cctgctcggc cgtcagtctg
      241 gtaaccaccc cgccgaaccc ggcgaccatg agctcggcga cctcctcgtc cagcttcttc
      301 ggcagcacct ccacggtgat gggcgcgcgc tggttctccg gctggtcggc ccagcgctgc
      361 tcgtagaggt gcatctgagc cagcacctga ttggcgaagg agccgtccat gacgcgcgac
      421 gggtggccca tggcgttgcc cagatttacc aggcggccct cggacagcag gatcaggtag
      481 tcgtggcgat cgtcgcttcg gaaaacctga tgcacctggt ccttgaccat ctcccagcgc
      541 cagtgctcac gcatgaaggc ggtatcgatc tcgttgtcga agtgcccgat gttgcagacc
      601 acggcgcctg ccttgacggt gctgagcatg ctggcatcgc agacgtcggc gttgccggtg
      661 caggtgacga tgagatcggt gtcgtgcagc aggcgggtat cgatgttctc gatgcggccg
      721 gtgttctcgc cgcccaggta gggagaaacc acctcgaacc cgtccatgca agcctgcatg
      781 gcgcagatgg gatcgatctc cacgacgcgc acgatcattc cctcctgacg gaggctctgg
      841 gccgagccct tgccgacgtc accgtagccg atgaccagcg cgcgcctgcc ggccatcagc
      901 atgtcggtgc cgcgcttgat ggcgtcgttc aggctgtggc ggcagccgta acggttgtcg
      961 ttcttggact tggtgaccga atcgttgacg ttgatggccg ggaccttcag ctcaccccta
     1021 tcgatcatct cgtagagccg gtgcacgccg gtggtggtct cctcgctgac gccatggatg
     1081 gagtcgagca tctcggggta cttctcgtgg atcacctcgg taaggtcgcc gccatcgtcg
     1141 agaagcaggt tggcgtccca cggcttgccg tccttgagaa tcgtctgctc gatgcaccac
     1201 cagtattact cctccgtctc gcccttccag gcaaaaacgg gtatgcccgc cgccgccatg
     1261 gccgcggcgg catggtcctg ggtggagaag atgttgcatg acgaccaacg gacttctgcg
     1321 cccagggcga ccagggtctc gatgagcacc gcagtctgca ccgtcatgtg aatgcagccg
     1381 atgaccttcg cgccggccag cggttgctgg tcggcgtagc ggttgcgaag cgccatgagc
     1441 gccggcatct cggcctcggc gaggttgatc tcgcgcctgc cgaaatcggc caggctcatg
     1501 tcggccactt tgtagtcagt catctgctct ttccttttcg atgtgttgtc tgaaatggtt
     1561 cgatgggttt ccgtatcccg gggctacgtg ccgttgcggg cgtagccgtg gatctgtatc
     1621 tgaaagccgt ttttctgggc caggtactgg gactcgaccg gcgccaggcc cgcctgggcc
     1681 gcccactgct ccagctcgcc gggctcgaag cccagccaca ggtcgccgca cgcgtcggtg
     1741 acccattcct gctcgtggcg atcgagctcc gccacgatga gcagaccgcc gtgccgcagg
     1801 atccgcgaag cgtggcggaa gaatctctgg ggcgacgcca ggtgatgcac gaccatggcc
     1861 gcgatcacgg cgcggtatcg gcgaatcgcc ggcagctcgg tgaaatcctt ctggcgcagc
     1921 ttgacgttgg gcagcccgtc cagacgctcg gccgcctggg cgagcatggt ggaggagctg
     1981 tcgattccga tcaggtcgtc gaagctccct gcaagcgtcg caagcagctg ccccccaccg
     2041 gggccgacct cgatgatcgg cccggaatcg acccctgcat ggtcgagcag ctcgacgatg
     2101 gctggaacgt atacctgcgc ttcgcagatg agcgcctgct gatcggcaaa acggtgtgcg
     2161 ttttcggcaa agaagttgtt gctgcgctgg cttcgctcgg cgtgaatctc cgccgcccgc
     2221 tctgccaggg cggcacccag gggggtgccg tcgacggttt cgaaaaggcg ctggcgcaat
     2281 ccgtcgtcgg gttcggtgca ccgccggtag tagacgttgt tgccctctcg acgtttcgcg
     2341 accaggtttg ccgtcagcag gactttcagg tgatggctga gcgccggttg gggaatctga
     2401 aagaggtggc acagctccgt gacgctgtag gatccgtcac tgagcacccg caggatgctc
     2461 gcccgcaggt gatcgcccac ggctttggcg acctcgacga cctcgtcgag gttggcttgc
     2521 tggcctgccg tctggcccat gggcggtggt gcctctacgc aacgagaggc gatcctatca
     2581 gcgccttcgc acaatatcaa aaaattttga tattgatggc ggaaagatcg ccgtgtcaga
     2641 atgcggcgat gagttggagc tcgccggatc tcgtcagcaa gctgccggac gtgcagacca
     2701 cgatcttcac ggtcatgtcc gcgctcgcca gggaggaggg cgcgctgaac ctttcccagg
     2761 gtttccccga tttcgatggc ccgcccgagc tcctcgaacg ggttcagtat tacctcaccc
     2821 acggtcacaa ccagtacgcg ccgatgacgg gcgtggcgcc cctgcgcgaa gccacggcgg
     2881 gcaagatcgc ggatctttac ggctgccagc tggatccgga gcaggaagtg accgtcacct
     2941 ccggcgccac cgaggcgctc ttctgcgcca tagcggcggt ggtgaatgcc ggtgacgagg
     3001 tgatcgtctt cgatcccgcc tacgacagct acgcgcccgt ggtgaagctc cagggcggcg
     3061 tgacccgcca cgttcccttg catccgccgg actatcgggt cgactgggat cgggtggcgg
     3121 acaacttgag cgatcggacc aggctgatca tcatcaacac gcctcacaac cccaccggaa
     3181 cggtctgggg cgaggacgac atcgaggcgt tgcgcgccgt gatcgacggc cgcgacctgt
     3241 ttcttctcgg cgacgaggtc tacgagcaca tcattttcga cgggcgccag cacgcgagcc
     3301 tgtgccgtta cccggatctt ttcgcccgca gcttcgtggt ctcgtcgttc ggcaagacct
     3361 atcacaccac gggctggaaa atcggctact gcgcggcccc cgcgccgctt tccgccgagt
     3421 tccgccggat tcatcagttc gtcaccttca cctccatcac gcccgttcag ctcggtctcg
     3481 ccgatttcat gacccggcat ccggagcacc atctcgggct cggcgcgttc tacgaggcca
     3541 agcgcgattt gttctgcagc ctgctggaag gctcccgttt cggccttacg aagagtgccg
     3601 gcacctactt ccagctgctc gactacggcg actactccaa cgaggccgat acggatctgg
     3661 ccgtgcgcct cacccgggag gcgaagatcg catccattcc gttgtcggtc ttctatgctg
     3721 acgatcccgg ccaccgcatg ctgcgtttct gctttgccaa ggatgacgct accttgcgag
     3781 ccggtgcgga gatactctta ggcctgtgaa gccgacgccg ccggatgagc gcgcgcgaac
     3841 gggatgaatc cgatgaccga catgaggaga aaaacggggt gaaacgtcca cttctggcat
     3901 tgctggtctg ctgcgcattt ggctgctcgg aatcatccaa cgagacgccc gcgtcgacgg
     3961 cggccgcagg tgacgggccg gccccagccg cacatccggg agaagagacc tacaaccgct
     4021 tctgcttttc ctgtcacgcc gccgggattg ccggagcgcc tcgaaccggc gacg
//
LOCUS       DBNHOX010000355         1727 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_670445, whole genome shotgun sequence.
ACCESSION   DBNHOX010000355 DBNHOX010000000
VERSION     DBNHOX010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_670445"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="AC16UZ_07785"
     CDS             <1..845
                     /locus_tag="AC16UZ_07785"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR6246622.1"
                     /translation="HNALEQEMFLRIAPELYLKRLLVGGFERVFEINRNFRNEGLSTR
                     HTPEFTMLEFYWAYADFEDLIALTEELLQLLARDVLGTGTLTYQGTEIDLARPAERYT
                     MVEAIERHTGIASGQLKDPDALAAILKGMNVQPEADWGIGKMQLELFEARVEDRLVQP
                     TFITHYPTEVSPLSRRNDEDPEVADRFELFIAGREIANGFSELNDPEDQAERFRAQAL
                     LRSGGDVEAMHFDDDYITALEYGLPPTAGEGLGIDRLVMLLTDSPTIRDVLLFPLMRP
                     VQDG"
     gene            838..1554
                     /gene="ung"
                     /locus_tag="AC16UZ_07790"
     CDS             838..1554
                     /gene="ung"
                     /locus_tag="AC16UZ_07790"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR6246623.1"
                     /translation="MVEAARRSEERVRLEPSWKAVLGGEFDKPYMNKLREFLLREKRA
                     GKVIYPRGDNIFAALDLTPLEDVKVVIIGQDPYHGPDQAHGLCFSVQPGIPQPPSLVN
                     IFQEINEELGGPAKRLDDTNGCLEPWARQGVLLLNAVLTVERGRAGSHQGMGWEIFTD
                     KVVDVLNRERDGLVFLLWGSYAQKKGAILDVQRHCVLKAPHPSPLSAHRGFFGCGHFA
                     RANEYLQQRGVAGIDWFDVA"
     gene            1610..>1727
                     /locus_tag="AC16UZ_07795"
     CDS             1610..>1727
                     /locus_tag="AC16UZ_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246624.1"
                     /translation="MIESPTEKLLVEQRGHTLLVTINTPPANTWDAESLQGLK"
BASE COUNT          331 a          527 c          556 g          313 t
ORIGIN      
        1 accacaacgc gctagaacaa gagatgtttt tgcgcattgc acccgagctg tacctcaagc
       61 gcttgttggt gggtgggttt gaacgcgtct tcgagatcaa ccgcaacttc cgcaacgaag
      121 ggttgtcgac gcggcacacc ccggagttca ccatgctgga gttctactgg gcctacgccg
      181 atttcgaaga cctcatcgct ctgaccgagg agttgctgca gcttctcgcc cgggacgttc
      241 tcgggacggg gacgctcacc tatcagggga cggaaatcga tctggcgcgg ccggcggaac
      301 gctacaccat ggtcgaagcc atcgaacggc acacgggcat tgcctcgggg cagcttaagg
      361 accccgatgc cctggccgcc attctcaagg gcatgaacgt tcagcccgag gcggactggg
      421 gtatcggcaa gatgcagctc gaactcttcg aggccagggt ggaagaccgc ctggtccagc
      481 ccacgttcat cacccactac cccaccgagg tatcgccgct gtctcgccgt aacgacgagg
      541 atcctgaggt ggccgaccgt ttcgagctct tcatcgccgg gcgcgagatc gccaacggat
      601 tttccgagct caatgacccg gaggatcagg ccgagcgctt ccgcgctcag gcgctgcttc
      661 gaagcggcgg cgacgtggag gccatgcatt tcgacgacga ttacatcacc gcgctcgagt
      721 acggtctgcc gcccacggcc ggcgaggggc tgggcatcga tcggctggtg atgctgctca
      781 cggactcccc gaccatccgg gacgtgctgc tgtttccgct gatgcggccg gtgcaagatg
      841 gttgaggccg cccgccggag cgaggagcgc gtccgcctgg agccttcctg gaaagcggtg
      901 cttggcggtg agttcgacaa gccctacatg aacaagctgc gggagttcct gctccgggag
      961 aagcgggcgg gcaaggtgat ctatccccgg ggcgataaca tcttcgccgc cctggacctg
     1021 acgcccttgg aggacgtcaa agtcgtgatc atcggtcagg atccctatca cggccccgac
     1081 caggcacacg gcctgtgctt ctcggtgcag cccggcatcc cgcaaccgcc atccctcgtc
     1141 aacatctttc aggagatcaa cgaggagctc ggcggtcccg caaagcgtct ggatgacacc
     1201 aacggctgtc tggagccgtg ggccaggcag ggcgtgctgc tgctcaacgc ggtgctgacg
     1261 gtggagcgtg gtcgcgccgg ctcgcatcag ggcatgggct gggagatctt taccgacaag
     1321 gtggtcgacg ttctcaaccg ggagcgggac gggctggtct ttctgctgtg gggaagctac
     1381 gcgcagaaga aaggagccat cctggacgtg caacggcatt gcgtcctgaa ggcgccccac
     1441 ccttcgccgc tgtctgccca ccgtggtttt ttcggctgcg ggcattttgc ccgggccaac
     1501 gagtaccttc agcaacgggg cgtggccggg atagactggt tcgacgttgc gtgatgcgac
     1561 tatctggaag aaaacaccga actgatgatc gactcaagag aggaagctga tgattgaatc
     1621 gccgactgaa aagctgctgg tcgagcagcg agggcacacg ctgctcgtca ccatcaacac
     1681 gccgcccgca aatacctggg atgcggaaag tctgcagggg ctcaaga
//
LOCUS       DBNHOX010000356         3220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_798973, whole genome shotgun sequence.
ACCESSION   DBNHOX010000356 DBNHOX010000000
VERSION     DBNHOX010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_798973"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1702)
                     /locus_tag="AC16UZ_07800"
     CDS             complement(155..1702)
                     /locus_tag="AC16UZ_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6246625.1"
                     /translation="MYPGKHVTEHAERPAIIMASTGEVITYKELEARTNQLAHLLRDQ
                     GLKRLDHYAIFMENNSRYVEACGAGERSGLYYTCINSYLTPEEVAYIVNNSESRVLIT
                     SREKAEVAKAALADCPRVALCLVVDGDDDARVVNYESAIERFPSTPIPDESLGTAMLY
                     SSGTTGRPKGIIRPLPEAPPEQDLPLFQFINGIWRFREGMIYLSPAPLYHSAPQGSVN
                     LTIRRGGTAVIMERFDPEQFLELVERYRITHSQLVPTMFSRMLKLPEEIRARADVSSL
                     EIAIHAAAPCPVQVKEAMIDWWGPIIYEYYGATEGLGFTGCTTEEWLAHKGTVGRVLL
                     GELHILDEDGNPSPKGEPGEVWFKTASAFEYFNDPEKTAQATSADGSMTTVGDVGYVD
                     DDGYLYLTDRATFMIISGGVNVYPQECENLLITHPKVADAAVFGVPNVDLGEEVKAVV
                     QVMPDVEPGEALEAELIAFCHEHLARQKCPRSVDFEEQLPRLPTGKLYKRHLRDRYWG
                     DKKSRIV"
     gene            complement(1722..2495)
                     /locus_tag="AC16UZ_07805"
     CDS             complement(1722..2495)
                     /locus_tag="AC16UZ_07805"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246626.1"
                     /translation="MSRTVQDLFDMSGHVAVVTGGGTGIGAGIARAFHESGAAVILAA
                     RRTENIERIAKEIRDDGGSAIAVTTDVTDDDAVDALARAAVEEFGRLTDWVNNAGGSP
                     MRMPLSELPREEWDRTIALNLTSIYVGCVTAARHMEHGSIINITSGAGTGPVPGSAHY
                     GASKAGTNSLTWTLAAEFAPRIRVNAVAPGAIPTEVMLKAIGRTEDELDKVLEEWNIP
                     LGRLGTPQDIGAACVYLASDAASWVSGEIIRVGGGARPR"
     gene            2614..3063
                     /locus_tag="AC16UZ_07810"
     CDS             2614..3063
                     /locus_tag="AC16UZ_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6246627.1"
                     /translation="MSENNAVEETKAVLGRRGHVTTSWDELETLQLSTGIETTMFRIG
                     MPDDPSAPTVFKVYYPPGCKVEAHTHECDYSEIVLEGSQKVGNEWLHPGDIRVGMANR
                     GYGPLVAGDEGATVLVIFADGQWPAVTIGRNDGSSLGVDELLKTYGR"
     gene            complement(3068..3205)
                     /locus_tag="AC16UZ_07815"
     CDS             complement(3068..3205)
                     /locus_tag="AC16UZ_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6246628.1"
                     /translation="MFDRTPLRREQTVEDIGNAAVFLASDDAMNITGQSLMVDGGMVK
                     V"
BASE COUNT          499 a         1027 c         1098 g          596 t
ORIGIN      
        1 cagcagcctc gacgccgttg tgggagagct ggtcgcaggc ggcagcctgg atcgcggcag
       61 cctgctggcg ttgctggagc cgaagctcac cgagccatca ggaattcttg ccgaactccc
      121 ccgttgaggc gggcgcccgc ctgcctgttt cagctcagac gatgcggctt tttttgtcgc
      181 cccagtaccg gtcgcgcagg tgacgcttat aaagcttgcc cgtaggcagg cggggcagct
      241 gttcctcgaa gtcgacggag cgggggcact tctggcgggc gaggtgctcg tggcagaagg
      301 cgatgagctc cgcttccagc gcttcccccg gttccacgtc cggcatgacc tggaccacgg
      361 ctttcacctc ttcgccgaga tccacgttgg gcacgccgaa caccgcggcg tcggcaacct
      421 tcggatgggt gatcagcagg ttctcgcatt cctggggata gacgttcacc ccgccggaga
      481 tgatcatgaa cgtcgcccgg tcggtgaggt acaggtagcc gtcgtcatcc acgtagccga
      541 cgtcgcccac ggtggtcatg ctgccgtctg ccgaggtggc ctgcgccgtc ttctcgggat
      601 cgttgaagta ctcgaaggcc gacgcggtct tgaaccagac ctcgcctggc tcgcctttcg
      661 gggaagggtt gccatcctcg tcgaggatgt gcagctctcc cagcagcacc cggcccacgg
      721 tgcccttgtg cgcaagccac tcctcggtgg tgcagcccgt gaagcccagg ccttccgttg
      781 cgccgtagta ctcgtagatg atggggcccc accagtcgat catcgcttcc ttgacctgca
      841 ccgggcatgg cgctgccgcg tggatggcga tctccaggga cgagacatcg gcccgggcgc
      901 ggatctcctc gggcagcttc agcatgcggc tgaacatcgt cggcacgagc tggctgtggg
      961 ttatgcggta acgttcgacc agttccagga actgctcggg gtcgaagcgc tccatgatca
     1021 cggcggtgcc gccccgccgg atggttaggt tgaccgagcc ctggggggcc gagtggtaga
     1081 ggggtgcggg cgagaggtag atcatgcctt cgcgaaagcg ccagatgccg ttgatgaact
     1141 ggaagagcgg cagatcctgc tccggcggtg cttccggcag gggccggatg atgcccttgg
     1201 gacgcccggt ggtgccggag gagtagagca tggccgtgcc gaggctctcg tcgggaatcg
     1261 gcgtcgaagg aaatctttcg attgcgcttt cgtagttgac caccctggcg tcgtcgtcgc
     1321 cgtcgacgac caggcacagc gctacccggg ggcagtccgc gagagccgcc ttcgccacct
     1381 ccgccttctc cctggaagtg atgagaacgc gggattcgct gttgttgacg atgtaggcca
     1441 cttcctccgg cgtgaggtac gagttgatgc acgtgtagta gaggcccgat cgctcgccgg
     1501 cgccgcaggc ttccacgtag cggctgttgt tctccatgaa gatggcgtag tggtcgagcc
     1561 ttttgaggcc ttggtcgcgc aggagatggg ccagctggtt ggtacgtgct tcgagctcct
     1621 tgtacgtgat gacctctccg gtcgaggcca tgatgatggc gggcctttcg gcgtgctccg
     1681 tgacgtgctt gcccgggtac atgacgaacc tccctcgacg gctagcgcgg gcgggcgccg
     1741 ccgcccacgc gaatgatctc gccgctcacc cagctggccg cgtccgaggc caggtagacg
     1801 caggcggcgc cgatgtcctg gggggtaccc agacgcccca gcggaatgtt ccactcctcc
     1861 agcaccttgt cgagctcgtc ctcggtcctg ccgatggcct tgagcatgac ctcggtgggg
     1921 atggcccccg gcgctaccgc gttgacccgg atgcggggcg cgaactcggc ggccagcgtc
     1981 caggtgagcg agttggttcc ggccttggag gccccgtagt gggcgctgcc cggcaccggg
     2041 ccggttccgg ccccagaggt gatgttgatg atcgacccat gctccatgtg ccgcgctgcc
     2101 gtgacgcaac ccacgtagat cgaggtgaga ttcaaggcga tggtcctgtc ccactcctcc
     2161 cgcggcagct cgctgagcgg catgcgcatg ggcgagcccc cggcgttgtt gacccagtcg
     2221 gtgagccggc cgaactcctc cacggcagcg cgggccagcg cgtcgacggc atcgtcgtcg
     2281 gtgacgtcgg tggtgacggc gatggcgctg ccgccgtcgt cacgaatctc cttcgcgatg
     2341 cgctcgatgt tctcggtccg gcgcgcggcg aggatcacgg cagcgccgga ttcgtggaag
     2401 gcccgggcga tgccggcacc aatgccggtg ccgcccccgg tgacgacggc cacgtgcccg
     2461 ctcatgtcga acaggtcctg aacggttctg ctcatgccca cgctctcctc ccccttggtg
     2521 cctgggatta tcacacaccg gccgcttcag gcgatcgttg agtgcccgtg ccggtcttgc
     2581 taacgttcgg gaattcttga gctggagcga tcagtgagcg agaacaacgc cgttgaggaa
     2641 accaaggccg tgctgggacg ccggggccac gtcaccacgt cctgggacga gctggaaacg
     2701 ctgcagcttt ccacggggat cgaaaccacg atgttcagga tcgggatgcc tgacgatccg
     2761 tccgccccga cggtattcaa ggtctactac ccgccgggct gcaaggtcga ggcgcacacc
     2821 cacgagtgtg actattccga gatcgtgctg gagggatctc agaaggtggg caacgagtgg
     2881 ctgcacccgg gggacatccg ggtaggcatg gcaaaccggg gctacggccc gctggtggcc
     2941 ggggacgagg gcgccacggt gctggtgata tttgccgacg ggcagtggcc ggcggtcacc
     3001 atcggccgca acgacggcag ctccctggga gtcgacgagc tcctgaaaac ctacggccgc
     3061 tgagccctca gaccttgacc atgccgccgt ccaccatcag ggactggccg gtgatgttca
     3121 tcgcgtcgtc cgatgccagg aagacggcgg cgttgccgat gtcctcgacc gtctgctccc
     3181 ggcgcagcgg cgttcggtcg aagatctccg gatagtcgcc
//
LOCUS       DBNHOX010000357         3757 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_799048, whole genome shotgun sequence.
ACCESSION   DBNHOX010000357 DBNHOX010000000
VERSION     DBNHOX010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_799048"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="AC16UZ_07820"
     CDS             <1..1309
                     /locus_tag="AC16UZ_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR6246629.1"
                     /translation="CGSRHHHVAVPVDRDAETVRRFKTLSPDLYRLADWLEACDIETV
                     AMEATGVYWIPLYEILEERGFEVVLVNARHVKNVPGRKTDVVDCQWIQELHSLGLLRG
                     SFRPSAEIASLRAYLRHRDKLVQDAASHIRRMQKALVLMNIQLHNVISDITGKTGMRI
                     VRDIASGATDPKVLASHRHDRIRASEEEIAASLTGNYRREHVFVLRQNLELYDSFQRQ
                     IEVCDGEIESLLQDLAAKRQPRKTALPAARRRHRIQNNEPRFEIRSPLHRLSGADITQ
                     IDALGPYTALRLISEIGTDMSRWPTEKHFTSWLTLAPKNKITGGRLISSKTQPSANRA
                     AAMLRMCAMSVGKTSTALGAYYRRIAYRIGKAKAITATARKLAILVYRVLRRDIDYAD
                     PGADAYEAQHRARTLRNLRNRAQRLGLGLIDLETGELMEAGVS"
     gene            1432..2502
                     /locus_tag="AC16UZ_07825"
     CDS             1432..2502
                     /locus_tag="AC16UZ_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013698.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIR6246630.1"
                     /translation="MERYIGLDPHGTSCTFGVIGPSGRKLRHDVVETNGAALVNYLRS
                     VPGRKHLCLEEGTQSAWLYEILSPHVEEIVVASIGESRGQKSDALDAFRRAEELRTGA
                     ITKPVFKAPRLYSRLRELARVHSMLVRDVVRAQARLKVMYRARGIATPGKSVYGPRQR
                     DRWLRQLPVSSRWATEKLHEEYDLLVELKREAEHELVVESRKHRISRVLATAPGLGPI
                     RVAQLLPIVVTPQRFRTRSQFWAYCGLGIVMRSSSDWVQSPNGGWVRAPVQRTRGLSR
                     NHNSNLKNIFKGAANTVVTRLHTDPLYEHYLRLTSAGTKPNLARLTIARRVAAIVLRM
                     WKDEQEYDPGKLKQATVAPAAR"
     gene            complement(3145..>3757)
                     /locus_tag="AC16UZ_07830"
     CDS             complement(3145..>3757)
                     /locus_tag="AC16UZ_07830"
                     /inference="COORDINATES: protein motif:HMM:NF023270.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HIR6246631.1"
                     /translation="DDFEKFGEVFLSESKQAGKQQYLVPYFIASHPGSDLHAMIGLAV
                     FLKRSGYRPDQVQDFIPAPFDIAAAMYHTGIDPFTRKPVFVARHLRDRKLQRALMQFF
                     KPENYFEVRKALLQAGRSDLIGGGCDALIPANPPREAILGRRRKANPRPSGEYVHAMP
                     RAGRTDPALGRNRKKGKKKQSRHEAEAGYRPQRKSTTRPRTRE"
BASE COUNT          719 a         1186 c         1203 g          649 t
ORIGIN      
        1 ctgcggatcg cggcaccacc atgtggccgt tcccgtcgat cgtgatgcgg aaacggtacg
       61 ccgattcaaa acgttgagcc cggacctgta ccgcttggcg gactggctcg aggcctgcga
      121 catcgagacc gtggcgatgg aggcgacggg cgtgtactgg attccgctct acgagatcct
      181 cgaggagcgt ggctttgagg ttgttctggt caacgcccgg cacgtgaaga acgtcccggg
      241 gcgcaagacc gatgtggtgg actgccagtg gatccaagaa ctgcatagtc tcgggctgtt
      301 gcgcggtagc ttccgcccct cggcggaaat tgcctcgctg cgcgcctacc ttcggcaccg
      361 ggacaagctg gttcaggacg ctgccagtca catccgacgc atgcaaaaag ccctggtcct
      421 gatgaacatc caactgcaca acgtgatcag cgacatcacg ggcaagacgg ggatgcggat
      481 cgtacgcgac atcgccagcg gagcgaccga tccaaaggtg ttggcgtccc atcgccacga
      541 tcgcattcga gcttccgagg aagagatcgc ggcctcgctc accgggaatt accgccgaga
      601 gcatgtcttc gtgctgcgac aaaacctcga gctgtatgac agctttcagc gccagatcga
      661 agtctgcgat ggcgagatcg agtccctgct tcaggatttg gcggcgaagc gacagccccg
      721 taagacagcg cttcccgcgg cgcgccggcg gcacaggatc cagaacaacg agccacgctt
      781 cgagatccgc agccctcttc accggttatc cggggcggac atcactcaga tcgatgctct
      841 cgggccgtac accgccctgc gactgatctc cgagatcgga accgacatga gccgttggcc
      901 caccgaaaag cacttcacgt cctggctcac gctggcgccc aagaacaaga ttaccggcgg
      961 ccgactgatc agctcgaaga cccagccctc cgcaaaccgt gctgccgcga tgctacgcat
     1021 gtgcgccatg tccgtcggta agacatccac agcgctcggc gcctactacc gccgcatcgc
     1081 ctaccgcatc ggcaaggcca aggccatcac cgccaccgct cgaaagctcg ccatccttgt
     1141 ctatcgcgtt ttgcgccgcg acatcgacta cgctgatccc ggagccgatg cctacgaggc
     1201 ccagcaccgc gcacgtacac tccgcaacct acgtaaccgc gcacaacgac tcggcttggg
     1261 gctgatcgat ctcgaaaccg gcgagctcat ggaagcgggg gtttcttaag agggtggcgt
     1321 cgacgagaca attcctgagc tgcggagcca tctcgggatg gcgtcctccg cgctattcgg
     1381 cgatcgttgg actgaacacc tcaacgccgc cacgagcgga ggaggacgcc agtggaaaga
     1441 tacatcggac tggatccgca tggtacgagt tgcaccttcg gcgtgatcgg gcccagtggt
     1501 cggaagttgc gtcacgacgt ggtggagacc aacggcgcag cgctggtgaa ctacctgcgg
     1561 agcgtccccg gccggaagca tctgtgcctg gaggagggga cgcagagcgc ttggctgtac
     1621 gagatcctct caccgcacgt cgaagagatc gtggtagcca gcatcggcga gagccgtggt
     1681 cagaagagcg acgcgctcga tgcgtttcga cgtgccgagg agcttcggac gggtgcgatc
     1741 acgaagccgg tgttcaaggc gccgcggctg tacagtcggt tgcgcgagct cgcgcgggtg
     1801 cactcgatgc tggtgcggga cgtggtacgg gcgcaggctc ggttgaaagt gatgtatcgg
     1861 gcccgaggta tcgccacacc gggcaagagt gtctacggtc cgagacagcg ggatcggtgg
     1921 ctgaggcagt tacccgtgtc ctcgcgttgg gctacggaga agctacacga ggaatacgac
     1981 ctgcttgtgg agctcaagcg agaggcggag cacgagttgg tggtcgagtc gcgcaagcat
     2041 cggatctcga gagttttggc cacggccccg gggttgggtc cgatccgggt ggcccagttg
     2101 ttgccgatcg tagtcacacc ccagcgattc cgcacgcgca gtcagttctg ggcttactgc
     2161 ggcctgggga tcgtcatgcg ctcgtcgtcg gactgggtcc agtcaccgaa cggagggtgg
     2221 gtgcgggccc cggtccagcg aacgcgtgga ctgagccgca accacaactc gaacctcaag
     2281 aacatcttca agggcgcggc caacaccgtc gtcacgagac tgcacaccga tcccctgtac
     2341 gagcactacc tacgactgac gtccgcgggt acgaagccca atctggccag gctgacgatc
     2401 gcccggcggg ttgccgcgat cgtcctgcgg atgtggaagg acgaacagga gtacgatcca
     2461 ggcaaactga agcaagcaac ggtcgctccg gcggcgcgct gatcgttcaa cccgcacgtg
     2521 ggcgtggtca gcgcaacagc ccgaggcgac acaaggtcgg aggtcagcat ccgagttctt
     2581 cctggactcg tcctgaatgg acgaagcccc cacggctagg ctatgcgcct ccacagtacc
     2641 gaacgaagca atggcccgaa gaggccctga tggaaggatg gtcccggtct cagaccggag
     2701 cacgcaactg cttgccgttc gccaaggctg ctgaacgtcc aacgcgcgaa gacgaacgat
     2761 caacacaaaa gacgcggggc agactcagcc cacgtaacgc tctgccgaat gcgggggtcg
     2821 acgccggatg caatacatcg gtggaaggag aagtgtctct tgacaccctc ctccatggaa
     2881 ggaagttcta tccgctcttc tcgattcagc gtccgataat ggccgatatg tcaacgcaga
     2941 ccaccccacc ccaatggctc gaatcggcca ttttctaccc cggcaggtgc accaggccag
     3001 cttcaacgat gtcagaatcc cgctctgacc tctgccggcg cggtgcgccc atgaggaaag
     3061 tacgactgaa accggcggcc tgaactcggt ggccaagttc acagaccccg actctgccgt
     3121 ttccgtggtc aaggccgtcg cttttcattc ccgcgtgcgc ggacgcgtcg tgctcttgcg
     3181 ctgcgggcgg tagccggcct cggcctcgtg ccgcgactgc ttcttcttac ccttcttcct
     3241 gttcctgccg agggcggggt ccgtacggcc ggcgcgcggc atggcgtgga cgtactcgcc
     3301 gctggggcgc gggttggcct tcctgcgccg gccgaggatc gcctcgcgcg gcgggttggc
     3361 cgggatcagc gcgtcgcagc cgccgccgat caggtcggag cggcccgcct ggagcagcgc
     3421 cttgcgcacc tcgaagtagt tctccggctt gaagaactgc atcagcgccc gctgcagctt
     3481 gcggtcgcgc aggtgcctgg cgacgaacac gggcttccgg gtgaacgggt cgatgccggt
     3541 gtggtacatc gccgccgcga tgtcgaacgg cgccgggatg aaatcctgca cctggtcggg
     3601 gcggtagccg ctccgcttga ggaagaccgc cagaccgatc atcgcgtgca ggtcgcttcc
     3661 cgggtggctg gcgatgaagt agggcaccag atactgctgc ttgcccgcct gcttcgattc
     3721 ggacaggaag acctcgccga acttctcgaa gtcgtcc
//
LOCUS       DBNHOX010000358         1945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_221238, whole genome shotgun sequence.
ACCESSION   DBNHOX010000358 DBNHOX010000000
VERSION     DBNHOX010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_221238"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="AC16UZ_07835"
     CDS             <1..662
                     /locus_tag="AC16UZ_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR6246632.1"
                     /translation="LERVVPGPVNLALWAALAIGAGGYLGLRAWSPRGMVSGAVRAAG
                     LLGVVYGALLLVGAAGGGNDPLRPLAGLTAWDPAHQPGEALEWQPVDDVAGLRAAVLA
                     ASREGKPVMLDLYADWCISCKVLERSVFPKPEVAARLSRFNLLRADVTANDDHHKALL
                     DELGVFGPPSMVFFAEDGRKLAEVTVQGEIGADALAAHLGAVLERVGSNNFSEIAVNF
                     R"
     gene            750..1181
                     /gene="aroQ"
                     /locus_tag="AC16UZ_07840"
     CDS             750..1181
                     /gene="aroQ"
                     /locus_tag="AC16UZ_07840"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR6246633.1"
                     /translation="MARFLVLHGPNLNLLGTREPEIYGTDTLEDINSRLEALAREAGN
                     EATCLQSNAEHELVDAVHGAKTQGVEGIVINPGAFTHTSVALRDALLGVGIPFIEVHL
                     SNVHAREEFRRQSYLSDVARGVITGLGAKGYEFALLAAMAR"
     gene            1194..1592
                     /locus_tag="AC16UZ_07845"
     CDS             1194..1592
                     /locus_tag="AC16UZ_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010795424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR6246634.1"
                     /translation="MDLRKIKKLIELVEESGIAELEVRDGEEAIRIARPVAGTVPAPV
                     AQVRAAQPGAVAGTITAPMAGTFYAASEPGAEPYLKVGQQVEVGDVVCIIESMKMMNE
                     IVADQSGTCVSVAVGNGQAVASGQVLFNIA"
     gene            1597..>1945
                     /locus_tag="AC16UZ_07850"
     CDS             1597..>1945
                     /locus_tag="AC16UZ_07850"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR6246635.1"
                     /translation="MSWLKFELDVEIDAADALSDRLLELGAVSVSLAPLSDELLLEPA
                     PGETPLWRRSRLSALFSADADVEALREFLAAHGDVDEGFVEDQDWVDAWRSSAVAECF
                     GGRLWVVPRDSEAR"
BASE COUNT          297 a          596 c          693 g          359 t
ORIGIN      
        1 tgctggagcg agtggtgccg ggcccggtga acctcgcgct gtgggcggcg ctggccattg
       61 gcgccggcgg ctacctgggc ctgcgtgcct ggagcccccg gggcatggtg tcgggcgccg
      121 tcagggccgc ggggctgctg ggcgtcgtct acggcgcttt gctgctcgtc ggggcggctg
      181 gcggcggcaa cgacccgctc aggcccctgg cgggcctcac cgcatgggat ccggcccatc
      241 agcctggcga ggcgctcgag tggcagcccg tggatgacgt ggcggggctg cgcgccgccg
      301 tgctggctgc cagccgcgag ggcaagccgg tgatgctcga tctctacgcc gactggtgca
      361 tctcctgcaa agtgctggag cgcagcgttt tcccgaagcc ggaggtggca gccaggttgt
      421 cgcggttcaa cctgctgcgg gcggacgtga cggcgaatga cgatcaccac aaagcgctcc
      481 tggacgagct tggcgtcttc gggcctccca gcatggtctt tttcgctgaa gatggtcgca
      541 aattagccga agttaccgtg cagggcgaaa tcggcgcgga cgcgctggcg gcccatctgg
      601 gggcggtcct ggaacgggtt ggatcaaata acttctctga aatagcggtt aacttccgtt
      661 aattaacgtc caaattcaca ttttatccgt attgtctttc cgcgtgggtt tctgcaaact
      721 cggcgccctg tgacgtctgg agtcctgcga tggcccgatt cctcgtgctt catggcccca
      781 acctgaacct gcttggcacc cgggaacccg agatctacgg caccgacacc ctcgaggaca
      841 tcaattcgag gctggaggcc ctggcccggg aggcgggcaa cgaggccacg tgcctgcaga
      901 gcaacgccga gcacgagctc gttgacgccg tgcacggggc caagacccag ggcgtggagg
      961 gtatcgtcat caatcccggc gccttcaccc ataccagcgt ggccttgcgg gacgccctgc
     1021 tcggggtggg cattcccttc atcgaggtgc acctttccaa cgtgcatgcc cgagaggagt
     1081 ttcgacgtca atcctacctg tcggacgttg cgcgcggcgt gatcaccggg ctcggggcga
     1141 agggatacga gttcgccctc ctggctgcca tggcgcgata ggagaggctg atcgtggatc
     1201 tcaggaagat caagaagctc atagagctgg tggaggagtc gggcatcgcg gagctcgagg
     1261 tgcgcgacgg ggaagaggcc atccgcatcg cccggccggt cgcgggcacc gtgccggcgc
     1321 cggttgccca ggtgcgggcc gctcagcccg gggccgttgc ggggaccatc acggcgccga
     1381 tggccgggac cttctatgcc gccagcgaac ccggcgctga gccttatcta aaagtgggcc
     1441 agcaggtcga ggtcggagac gtggtctgca tcatcgagag catgaagatg atgaacgaga
     1501 tcgttgccga ccagtccggt acctgcgtgt cggtcgcggt cggcaacgga caggccgtgg
     1561 catcgggcca ggtgctgttc aacatcgcct gacccggtga gctggctaaa gttcgagctc
     1621 gatgtcgaga tcgacgccgc cgatgccctg agcgatcgcc tgctggagct gggcgcggtg
     1681 tccgtgagcc tggcgcccct gtccgatgag ctgctcctcg agcctgcccc cggtgaaacc
     1741 cctctgtggc gtcgtagccg cctgtcggcc ctgttctccg cggatgccga cgtggaggcg
     1801 cttcgtgagt tcctggcggc ccatggcgac gtcgacgagg gcttcgtcga ggatcaggat
     1861 tgggtggatg cctggcgctc gagcgcggtg gctgaatgtt tcggcggccg tttgtgggtg
     1921 gtcccgcggg attctgaggc gcgca
//
LOCUS       DBNHOX010000359         1700 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_157309, whole genome shotgun sequence.
ACCESSION   DBNHOX010000359 DBNHOX010000000
VERSION     DBNHOX010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_157309"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="AC16UZ_07855"
     CDS             complement(<1..816)
                     /locus_tag="AC16UZ_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246636.1"
                     /translation="MSETGPAPQERGKSNILLRIATWLITAGCFYLVYGRTAEAAARD
                     GLTVLEYLARFFGEANWLAWLAVMVPYSVFFFVVDAHVTWRVIRWFNAPEIRLGNVLP
                     ISASAYILSLVNEQVGKGAMSLYLYRRYKVPALAAISSMLLLGLMEIYQLLVFSSVGV
                     FLGFEVVQAASTELPLDKILPGVFAVAAIYLPLHIAYFRGHLLKGSTVREKSLVRAFR
                     EARPIHYLLILLFKAPLLIGAIVVYTIALNLFNVDVSFAQMLAFLPVIFLAAAL"
     gene            complement(860..>1700)
                     /locus_tag="AC16UZ_07860"
     CDS             complement(860..>1700)
                     /locus_tag="AC16UZ_07860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR6246637.1"
                     /translation="AQQAGVRIVHTCGFDSIPSDIGVLCVQREMKSRHGEVATEVKSR
                     VRGMRGGFSGGTVASMLNMMEESEMDPGVREVMNDPYGLNPAGERHGLDGPEHSVPEY
                     DPDFGAWTTPFVMAAINTKVVRRSNALSGYAYGRDFRYDEAMLTGFGPWGFPLAAAMS
                     VGSTAFNAAAGVSALRGLLARVLPEPGEGPSAAARERGFFDIEMLGKSRRSGARVKVR
                     IRGDRDPGYGSTSRMLGESAVCLARDDLETPTGVLTPAVAMGEALIGRLGNNAGVTFE
                     VMP"
BASE COUNT          336 a          554 c          548 g          262 t
ORIGIN      
        1 cagcgccgcg gccagaaaga tcacgggaag gaacgcgagc atctgagcga agctgacgtc
       61 gacgttgaag aggttcaagg cgatggtgta gacgacgatc gcaccgatca gaagcggcgc
      121 cttgaacagc aaaatgagca ggtagtgaat gggccgggct tcacggaatg cccggaccag
      181 gctcttctcc cgaaccgtcg atcccttcag tagatgtccc cggaagtagg cgatgtgcag
      241 cggcagatag atggccgcca cggcaaacac gccgggcagg atcttgtcga gcggcagctc
      301 cgtggaggcc gcctggacca cctcgaatcc gagaaagacc ccgaccgagg agaacaccag
      361 caactggtag atttccatca gcccgagcag cagcatgctg gagatggccg ccagagcagg
      421 gactttgtaa cggcgataga ggtagaggga catcgcgccc ttgcccacct gctcgttgac
      481 cagggacagg atgtaggcgc tggcactgat cggcagcacg ttgcccagcc ggatttcggg
      541 ggcattgaac cagcggatga ctcgccaggt cacgtgagcg tcgacaacga agaagaaaac
      601 ggagtagggg accatgaccg cgagccaggc aagccagttg gcctcgccga agaagcgggc
      661 caggtattcc aacacggtca ggccgtcacg tgccgccgct tcggccgtcc ggccgtaaac
      721 gagatagaag cagccggcag taatcagcca ggtcgcaatc cgaaggagga tgttggattt
      781 gccgcgttcc tggggagccg gcccggtctc gctcacgccc acgcgctcct ttcgttagat
      841 gagcgacgaa gtgagcgcct cacggcatca cctcgaacgt cacgccggcg ttgttgccaa
      901 gacgcccgat cagcgcttcg cccatggcca cggccggggt aagcacgccc gtgggtgtct
      961 ccagatcgtc tctggccagg cagacggcgc tctcgcccag catccgcgag gtcgagccgt
     1021 agcccggatc acgatccccg cggatcctga ccttgacccg cgcaccgctg cggcggctct
     1081 tgccgagcat ctcgatgtcg aagaatcccc gctcgcgggc agcggccgag ggcccctccc
     1141 cgggctcggg cagaacccgc gccagcaatc cacgcagcgc agaaaccccg gcggcggcgt
     1201 tgaaagccgt cgagccgacg ctcatcgccg cggccagcgg gaagccccag gggccgaagc
     1261 ccgtcagcat cgcctcgtcg taccgaaagt cgcgcccgta ggcgtagccg gacaacgcgt
     1321 tgctgcggcg aacgaccttg gtgttgatgg ctgccatgac gaacggtgtg gtccatgcgc
     1381 cgaaatccgg gtcgtattcg ggcaccgagt gctcgggccc atcgaggccg tgacgctcgc
     1441 ccgcggggtt gaggccatac ggatcgttca ttacctcccg aacgcccggg tccatctcgc
     1501 tttcttccat catgttgagc atgctggcca cggtcccgcc gctgaagccg ccgcgcattc
     1561 cgcgcacgcg ggacttgacc tcggtggcca cctcaccgtg ccgggatttc atttcccgtt
     1621 gcacgcagag cacgccaatg tccgacggta tgctgtcgaa gccgcacgtg tggacgatcc
     1681 gaaccccggc ctgctgggca
//
LOCUS       DBNHOX010000360        13772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_27384, whole genome shotgun sequence.
ACCESSION   DBNHOX010000360 DBNHOX010000000
VERSION     DBNHOX010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13772
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_27384"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="AC16UZ_07865"
     CDS             <1..1244
                     /locus_tag="AC16UZ_07865"
                     /inference="COORDINATES: protein motif:HMM:NF020260.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair MutL family protein"
                     /protein_id="HIR6246638.1"
                     /translation="ISLCRFDVAFELRHGGTRSVLSLPAGDPELRLARVIGEAFVKRS
                     VALDETRGELRLWGWVGLPTYSRSQADQQYFYVNGRVVRDKLVAHAVRQAYRDVLFGG
                     RQPVFSLYLELPADAVDVNVHPTKHEVRFREARSVHDFIFGTLARALREVRPDAVAAP
                     VAGEAAATGSRGSWTQGRQAGLGLPHAVQDRPFPPAPRSLQEAFAEHGAPITVTESDQ
                     PIPPLGYAVAQLHGVYILAQNADGLVVVDVHAAHERVTYEKLKRQMDTEGVPQQRLLV
                     PVPVSVSARDADLLEQFEDVVSATGLSLDRSGTQSLMVRAVPALLAGANAEELVRDVL
                     ADLAELGSSEQVRNRQHDLLATMACHGSVRANRELTLAEMNALLREMEVTDNAGQCNH
                     GRPTYMVQSMAELDRLFLRGQ"
     gene            1258..2232
                     /gene="miaA"
                     /locus_tag="AC16UZ_07870"
     CDS             1258..2232
                     /gene="miaA"
                     /locus_tag="AC16UZ_07870"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014860414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR6246639.1"
                     /translation="MTVPASGSVALVITGPTAVGKTELAIEIARRVPASLISMDSALV
                     YRGMDVGTAKPSPESLIEHPQALIDVRDPKEPYSAAEFVADADSLVQEAWAAGRVPVV
                     VGGTMMYLRAFREGLADLPQADPQLRRAIAAEAESRGWEALHEQLRAVDPAAAQVIHP
                     NNPQRLQRALEVHRLTGRPISDFWSEQRNADFAARLGGRMETIALMPDRRDRLHARIA
                     RRFAGMLERGLVEEVRQLRARGDLHADLPSMRAVGYRQVWQHLEGELDAAEMRERACA
                     ATRQLAKRQLTWLRGWSWVREVEWGDPVALAEQALLRADQPHGSARRA"
     gene            2344..2592
                     /gene="hfq"
                     /locus_tag="AC16UZ_07875"
     CDS             2344..2592
                     /gene="hfq"
                     /locus_tag="AC16UZ_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HIR6246640.1"
                     /translation="MSKGHSLQDPYLNTLRKERIPVSVYLVNGIKLQGQIESFDQFVV
                     LLRNSVSQMVYKHAISTVVPSRNVRLSGPGGSEEAAEE"
     gene            2647..3996
                     /gene="hflX"
                     /locus_tag="AC16UZ_07880"
     CDS             2647..3996
                     /gene="hflX"
                     /locus_tag="AC16UZ_07880"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HIR6246641.1"
                     /translation="MFFERPEAGRRSVLLQVHLKSAAAPDAAVLAESELEAIELARSA
                     GVDVCQVVSARRDNPHPKWFVGPGKVAELAEVLASQEADLLIVNNELNAGQQRDLERD
                     LLCRVLTRTELILHIFADRARTYEGQLQVELAQLRHAQTRLVRGWTHLDRQKGGIGLR
                     GAGETQIEMDQRALGEHIKRVQRKLEQVARRRDQNRRRRRRTRTRTVSLVGYTNAGKS
                     TLFNALTRAGVLVEDKLFATLDPTMRRLTVPGAGDVVLSDTVGFINQLPHDLVDAFKA
                     TLEEVSNADLLLHVVDAGCRDIAERIGQVREVLAEIGASDLPTILVFNKIDQLTPAER
                     RAVLAADVWSAGDGNAMAVAVSAVTGEGLDELTEAIGRRLGVAPEPAKVVLPADDGKT
                     RAWLYRMGAVLGEEITEDGRVALRVRADADLLERLGRTPRVLLQVNDAVPRILPLPN"
     gene            4018..5154
                     /gene="hflK"
                     /locus_tag="AC16UZ_07885"
     CDS             4018..5154
                     /gene="hflK"
                     /locus_tag="AC16UZ_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003380712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIR6246642.1"
                     /translation="MAWNEPGGGDKDPWGSRGDQGPPDLDEAFKKLQAQLASIFGGGR
                     GGSGGDGSGLNAGMLGIVLVVAAVAYGLFGLYQLDEQERGVVFRFGAAKEDIVLPGLH
                     WNPPIIDVVRKVNVTRVNSKNHQALMLTEDENIVDVSLTVQYVVNDPLKFLVMVRDPE
                     NSLDHAVESALRHVVGSSLMDDVITAGRAAIAAEVQDRLQTYLDTYGTGILVSKVNID
                     ESAPPNQVRDAFNDVQKAKEDEQRVINEANAYAESIIPEARGEAQKQIEQANAYRDQV
                     IARAEGEAERFEKLLTEYSLAKEVTRDRLYIDAMESVLSRSSKIMIDVEGGNNLLYLP
                     LDRLGQTGSPVAGLATDESVRSIADAILREVNERIASGRIRESR"
     gene            5163..6029
                     /gene="hflC"
                     /locus_tag="AC16UZ_07890"
     CDS             5163..6029
                     /gene="hflC"
                     /locus_tag="AC16UZ_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HIR6246643.1"
                     /translation="MNLKNLIIVVIAIIAVLLLMNSVYTVQETERAILLRFGAVSQAD
                     VPPGLHFKMPIAEEVKKADGRVLTLDSVPETYYTLEKKPLIVDSFAKWRIVDVQRYYT
                     GTSFDERRGQRVLQERVNEGLRNQISRRDMHEVVSGERDQLMQELLGDLNAVMSGAVG
                     IEVIDVRVKRIDLPPDVSSSVYDRMNSERQIEARQYRAQGRELALGIRADAERQTVII
                     EAEAYRSSEQIRGDGDARSANIYASAYNRDPEFYQFYRSINAYAKVFKDKSDILVLDP
                     TSEFFKYLANDS"
     gene            6081..7295
                     /locus_tag="AC16UZ_07895"
     CDS             6081..7295
                     /locus_tag="AC16UZ_07895"
                     /inference="COORDINATES: protein motif:HMM:NF009086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR6246644.1"
                     /translation="MVNRKWRLPDGVDELLPPEAWALEQVRRQVLDVFNSWGFDYVEP
                     PVVEYLDALLVGAGPDGGLGHQDDLDLQTLKVVDQRSGRLMGVRADMTAQAVRIDAHS
                     RPSEGVQRLCYAGPVVLANPAAALDSRVPLKAGAEVFGAPGLAADVEVICLLLEILER
                     TGVEAPVLVLGHMGIYLSLVAPLGLDAFTERQLFAAVQSKSEADIGELIGERSDGALL
                     ERLPTLMGGREILQEARAALAGGPDGAVAAVDALEELAEAVLVRNPAVEVRYDLAELA
                     GYGYHNGPVFSAYQADQGRAIARGGRYDGIGAPFGRSRPATGFDVNLRQLIGLAGAGT
                     VGQAVWAPWTGSLGGDVAAALAAEVRRLRAAGERVVVALDDEDGAPSGCDRQLSYVSG
                     VWKVERLGRNEG"
     gene            7297..8592
                     /locus_tag="AC16UZ_07900"
     CDS             7297..8592
                     /locus_tag="AC16UZ_07900"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR6246645.1"
                     /translation="MGRNVVVMGTQWGDEGKGKIVDLLTDQVAAVVRFQGGHNAGHTL
                     VIEGEKTVLHLIPSGILRREVQCFIGNGVVLEPNALLSEVSGLEARGVPVRERLRISP
                     ACPLILPYHVKLDLAREEARGNQKIGTTGRGIGPAYEDKAARRGVRLGDLFYWQEFAA
                     KLAEVMEYHNFMLERYYGSETVDYAQTLDECAAVAEQIRPMVADIIPLLHEIRESGAN
                     LLFEGAQGALLDIDLGTYPFVTSSNTTAGGTAVGSGFGPRYLDYILGITKAYSTRVGS
                     GPFPTELFDEVGERLATRGNEFGSTTGRPRRCGWFDAVALRQAVQINSISGLCLTKLD
                     VLDGLESICICVGYEGPDGTSGTPRFASEYYSEIRPVYEEVDGWSESTLGIRRLDDLP
                     ANARAYLARIEETVGAPIDIISTGPDRTETIVLKDPFTA"
     gene            8620..8811
                     /locus_tag="AC16UZ_07905"
     CDS             8620..8811
                     /locus_tag="AC16UZ_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008375064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR6246646.1"
                     /translation="MDSKLLELLVCPVTKASLTYNKDKDELWCRASGLAYPIRDGIPV
                     MLEEEARELSAEERESLRE"
     gene            8948..9898
                     /gene="folE2"
                     /locus_tag="AC16UZ_07910"
     CDS             8948..9898
                     /gene="folE2"
                     /locus_tag="AC16UZ_07910"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HIR6246647.1"
                     /translation="MNKGELRSEKELPDVASHELHQPHVTLDWVGMADIHQPLLVRDG
                     NQTRHVQSRVQIYVDLGDELAKGIHMSRLYLILDEHAETRPLSAAGLKLLLRSVLESH
                     RELSSRAYVQFDFDYYLRRQALVSDNSGWNSYPVSIRGTLIDGEITLEVALNVQYSST
                     CPCSAALARQLIQDQFDEDFGTSGSVAVGDVRAWLGTEQGIVATPHSQRSDARIRVKL
                     REGLIDFPITDLIDEVEATLKTPVQTAVKREDEQEFARLNGQNLMFIEDAGRKLKDQL
                     DADDRLADFWVRVEHHESLHAHDAVGVFTKGVGDGYQAVP"
     gene            9899..11032
                     /locus_tag="AC16UZ_07915"
     CDS             9899..11032
                     /locus_tag="AC16UZ_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011332951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR6246648.1"
                     /translation="MSKPVVISGAGLWKPDHVITNAELVAAYNAYADRFNAENDAAIG
                     AGDVAPMEHSSEEFIEKASGIKQRYAYVKDGILEVGRMRPRIPHREEDELSHQAEIAL
                     HAARGAMAAAGKAASDIDMVIVSCAYTERSYPAIAIEVQHALGIDGFAFDMLVACSAA
                     TFALQRAHDAIAAGSARCVLVVNPELTSPQVNYRDRDGHFIFGDVSTAVVLESAETCT
                     AAHAYEILGSKALTSYSNNIRSNFGYLAYAEDVDPFCAANLFHQEGRRVFKEVCPMAA
                     EHLKSQVEAVGLDVADVKRWWLHQANLNMNLLISKRLLGRDASAKEAPIVLDEYANTA
                     SAGSIIAFCLHHEDLAAGDHGVICSFGAGYSIGSLVLRKRAAA"
     gene            complement(11095..11181)
                     /locus_tag="AC16UZ_07920"
     tRNA            complement(11095..11181)
                     /locus_tag="AC16UZ_07920"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11145..11147),aa:Leu,seq:cag)
     gene            11228..13429
                     /gene="rnr"
                     /locus_tag="AC16UZ_07925"
     CDS             11228..13429
                     /gene="rnr"
                     /locus_tag="AC16UZ_07925"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIR6246649.1"
                     /translation="MATRTKKTNSSSKITARDVRESLGRGRGPLAWRELVAATVGDSA
                     SGRRELRRVLRGLVSAGEVVQIPDGRYQLEEPAGVERGVVALHRGRLCVGEIPLETGR
                     RVRLRPGDVVEMQIGQGADGDIARVLRVIEYSAEPIIGQLNGRGRHPYVDSLSPHYKG
                     RVSLVDPPAFGNHGDTVSVRIVDEDRRGLVGYVEALVSDRRGAAHAAETMLASHGVPR
                     QWPSAVLSAAQRLPKAVVPARHPDRRDLAGLPLVTIDGETARDFDDAVYAARRRGGWR
                     LVVAIADVAHYVKPKGALDAEALERGNSVYLPDRVVPMLPEAISNGLCSLKPREPRLA
                     MVCDMQVSNAGNVTRFEFYEAVIRSWQRLTYKRVQEFLDSGALDVEPEVCRSLADLQG
                     TFSALRVARDQRGALDFDTHEATLELSGDHVAAIHPFIRTDAHRLIEEAMIAANVCAA
                     TFVEKHGVSALYRVHEGPSGERDQRVRQAFAYAGIRLPRGRLDPAMLRQALDGLGERD
                     DRWVFEMLVLRSLTQAVYRPENKGHFGLALTRYMHFTSPIRRYADLVVHRAIKAALHG
                     APPPAADDWLVACGEQVSMTERRADEVSWAVDGWLKCEYVSSRIGEVFPGVVMGVTDF
                     GLFVELTGYYVQGLLHVSDLGSDYFQYQPATLALVGERSNRRFGLGDHLTVRLAGVQP
                     ALGRLDLELVEPAAGSASPRTNRRRPAADRETGRGGARGGARGGPRGRKRR"
     gene            13426..>13772
                     /locus_tag="AC16UZ_07930"
     CDS             13426..>13772
                     /locus_tag="AC16UZ_07930"
                     /inference="COORDINATES: protein motif:HMM:NF019644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR6246650.1"
                     /translation="MTDGSKVHGIHAVTAVLRDDPARVRVVYCITGRVDARMREVLEE
                     ARAAGVRVERVDRRQLDRLAGSGHQGVAAHCHELALADERAFAEHLDTLPEPWLMLAL
                     DGVTDPRNLGACLR"
BASE COUNT         2361 a         4277 c         4761 g         2373 t
ORIGIN      
        1 gcatcagcct gtgccggttc gacgtcgcct tcgagctgcg ccatggcggc acccggtcgg
       61 ttctgtcgct gccggcagga gaccccgagc tgcgacttgc ccgggtaatt ggcgaggcct
      121 tcgtgaagcg ctcggtggcc ctggacgaga cgcgcgggga gctcaggttg tgggggtggg
      181 tcgggttgcc cacctattcc cgcagccagg cggatcagca gtatttctat gtgaacgggc
      241 gcgtggtgcg agacaagctc gtcgcccacg ccgttcgcca ggcttaccgg gacgtccttt
      301 tcggtggccg gcagccggtc ttttcccttt acctggagct gccggcggac gccgttgacg
      361 tgaacgtgca cccgacgaag cacgaggtcc ggtttcgcga ggcgcggtcg gttcacgatt
      421 tcatcttcgg tactctggcc agagcgcttc gcgaggtgcg gccggatgcc gtggccgcgc
      481 ctgttgcggg cgaagcggcg gcgaccggtt cccgggggtc gtggactcag ggtcgccagg
      541 ccgggctggg tctgcctcac gcggtccagg atcggccttt tccgccggcc ccgcggagcc
      601 tgcaggaagc gttcgccgag cacggcgcgc cgatcacggt cacagaatcc gaccagccga
      661 ttccgccgct cggctatgcc gtcgctcagc tgcacggtgt ctacatcctg gctcagaacg
      721 cggacgggct ggtcgttgtg gacgtgcacg cggctcacga gcgcgtgacc tacgaaaagc
      781 tcaagcgaca gatggatacg gaaggcgtgc cgcagcaacg tctgctggtt cccgtgccgg
      841 tgagcgtcag cgcgcgcgat gcggatctgc tcgagcagtt cgaggacgtc gtctcggcca
      901 ctggtctttc cttagacaga tccgggacgc agagcctgat ggtgcgcgcg gtccccgcgc
      961 ttctggccgg cgccaatgct gaggagctcg ttcgcgacgt cctggcggac ctggccgagc
     1021 tcggttcaag cgagcaggtc cggaaccggc agcatgatct gctggctacc atggcctgcc
     1081 acgggtccgt gcgtgcgaac cgtgagctca ctctggctga gatgaacgcg ctgctgcggg
     1141 agatggaagt cacggacaac gccgggcagt gcaatcacgg caggcccacg tacatggttc
     1201 agtccatggc ggagctggac cggctattcc ttcgcgggca gtagacccat cgatcccatg
     1261 acagtaccgg catcgggttc cgtggcgctc gtgatcaccg ggccaacggc ggtgggcaag
     1321 accgagcttg ccatcgagat cgcccggcga gtgccggcca gcctgatcag catggattcc
     1381 gccctcgttt accgtggcat ggacgtcggc acggccaagc cttcaccgga gagcttgatc
     1441 gagcaccccc aggctctgat cgacgtccgt gacccgaaag agccatactc ggctgcggag
     1501 ttcgtcgccg atgccgacag cctcgttcag gaggcctggg cggcgggtcg cgtgcccgtc
     1561 gtggtgggcg gcaccatgat gtatctgcga gcatttcggg aaggtctcgc cgatctgccc
     1621 caggccgatc cgcagctgcg cagggccatc gctgccgagg cggagtcccg gggatgggag
     1681 gctcttcacg agcagctgcg cgccgtggat ccggccgcgg cgcaggtcat tcatccgaac
     1741 aacccccaac gcctgcagcg ggcgctggag gttcaccgcc tcaccggccg gcccatatcc
     1801 gacttctggt cggagcagcg caacgccgat tttgcggcgc gccttggcgg gaggatggag
     1861 accattgccc tgatgcccga ccgtcgtgac cggcttcacg ctcggatcgc ccgtcgattt
     1921 gcagggatgc tggagcgggg gctggtggaa gaggtgcggc aattgcgcgc tcgcggcgat
     1981 cttcacgcgg accttccgtc catgcgggca gtgggttatc gccaggtctg gcagcatctc
     2041 gagggcgagc tcgacgcagc cgagatgcgc gagcgtgcgt gcgcggcgac gcggcagctc
     2101 gccaagcgtc agctcacgtg gctgcggggg tggtcgtggg tcagggaggt cgagtggggc
     2161 gacccggttg cgctggcgga gcaagccctg ctgcgcgcgg accagcccca cggaagtgct
     2221 cgacgcgctt gaaatatggg caggctgtag ccatagtgaa tgtggcggta aaaggacgag
     2281 cgctgcgtcc ccgaaaagtc ctgcaaagcc gtcgattggg gggcgaactt gaaggagctg
     2341 caaatgtcaa aagggcattc gttacaagac ccttatctca acaccctgcg caaggagcgc
     2401 attcccgtat cagtttatct cgtcaacggt atcaagctgc agggccagat cgagtcgttc
     2461 gatcagttcg tggtcctgct gcggaactct gtgagccaga tggtctacaa gcacgccatc
     2521 tcgaccgttg tgccttcgcg caacgttcgc ctgtcggggc ccggcggctc cgaagaggct
     2581 gcggaggaat agaccggttc aggccggctc ttcccttggc ggaaggcggg cctgaaagga
     2641 gcacacttgt tttttgaacg acccgaagca ggccgtagat cggtcctgtt gcaggttcac
     2701 ctgaagagcg ctgcggcgcc cgatgcagcc gtccttgccg aatcggagct cgaagccatc
     2761 gagcttgccc gttccgctgg agtcgacgtc tgtcaggtgg tctcggcgcg gcgggataac
     2821 cctcacccga agtggttcgt cggccccggc aaagtcgccg agctcgccga ggtgcttgcc
     2881 tcgcaggaag ccgatctcct tatcgtcaac aacgagctga acgccggcca gcagcgcgat
     2941 ctcgagcgcg atctgctctg ccgggtgctc acgcgtaccg agcttatcct gcacatcttt
     3001 gcggatcgtg cccgaaccta cgagggtcag ctgcaggtgg agctggcgca gctcagacat
     3061 gcccagaccc gcctcgtccg gggttggacg catctggatc gtcagaaggg tggcatcggc
     3121 ctgcggggtg cgggcgaaac ccagatcgag atggaccagc gggcgctggg ggagcacatc
     3181 aagcgggttc agcgcaagct ggagcaggtg gcacggcgtc gcgatcagaa ccggcgccgt
     3241 cggcgccgca cccgcacccg cacggtctcg ctggtgggat acacgaacgc cggcaagtcg
     3301 accctgttca acgcgctcac cagagccggg gtgctcgtcg aggacaagct gttcgccact
     3361 ctggatccaa ccatgcggcg actgacggtt cccggcgcgg gcgacgtcgt gctgagcgac
     3421 accgtcggct tcatcaatca gctccctcac gacctggtcg atgccttcaa ggcgacgctc
     3481 gaagaggtca gcaatgccga cctgctgctg catgtggtgg acgccgggtg cagggacatc
     3541 gccgagcgga tcgggcaggt ccgggaggtt ctcgccgaga tcggagcgtc cgacctgccg
     3601 acgatcctgg ttttcaacaa gatcgatcag ctcacgccgg ccgagcgccg ggcggtgctg
     3661 gccgccgatg tctggtcggc cggggacggg aacgccatgg cggtagcggt cagcgcggtc
     3721 actggggagg gcctggacga gctcaccgaa gccatcggaa gacgcctcgg cgtcgccccg
     3781 gagcccgcca aggtggttct gcccgccgat gatggcaaga cgcgcgcctg gctgtatcga
     3841 atgggcgccg tgcttggcga agagatcacc gaggacggac gggttgcgct gcgggttcgt
     3901 gcggacgccg atctgctgga gcgcctgggc cgcacgccca gggttctgtt gcaggtcaat
     3961 gacgccgtcc ctagaatctt gcccctgccc aattaatctt taacggagca ccacgagatg
     4021 gcctggaacg agccgggtgg aggtgacaag gacccgtggg gaagccgcgg agaccagggc
     4081 ccacctgatc tggatgaagc tttcaagaag ctgcaggccc agcttgcctc catttttggg
     4141 ggtgggcgcg gcggcagtgg tggcgatggc agcggtttga atgcggggat gctgggcatt
     4201 gtgctcgtcg tcgcggccgt agcctacggt ctgttcggcc tgtatcagct cgacgagcag
     4261 gagcgagggg tggtgttccg gttcggggcg gccaaggagg acatcgtcct cccgggactg
     4321 cactggaatc cccccatcat cgacgtggtc cgcaaggtca acgtcaccag ggtgaactcg
     4381 aagaatcatc aggccctgat gctgaccgag gacgagaaca tcgtcgacgt cagcctgacc
     4441 gttcagtacg tggtcaacga cccgctgaaa tttctggtca tggtccgcga ccccgagaac
     4501 agcctcgatc atgccgtgga aagcgcgctg cgccacgtcg tgggaagctc cttgatggac
     4561 gacgtcatca ccgcgggccg ggcggccatc gcggccgagg tccaggacag gcttcagacc
     4621 tatctggata cctacggcac gggcattctc gtgagcaagg tgaacatcga cgagagcgcg
     4681 ccgcccaacc aggtgcgcga cgcgttcaac gacgtccaga aggccaagga ggacgagcag
     4741 cgcgtcatca acgaagccaa cgcttacgcg gaaagcatca ttcccgaggc caggggtgaa
     4801 gcgcagaagc agatcgagca ggccaacgcc taccgcgatc aggtgattgc tcgcgccgag
     4861 ggtgaggcgg agcggttcga gaagctgctc accgagtaca gcctcgccaa ggaagtgacc
     4921 cgcgaccggc tctacattga tgccatggag tcggtgctca gccgatcgag caagatcatg
     4981 atcgacgtcg agggaggcaa caatctcctg tatctgcctc tcgacaggct cgggcagacc
     5041 gggagcccgg tggccggcct ggccacggac gaatcggtca gaagcatcgc agacgccatt
     5101 cttcgtgaag tcaatgaacg catcgccagc gggcggattc gggaaagccg ataggatccg
     5161 acatgaatct caagaatctc attatcgtcg tcatagccat catcgccgtg ctgctgctga
     5221 tgaacagcgt ctacacggtg caggagaccg aacgcgccat cctgctccgc ttcggcgcgg
     5281 tcagccaggc ggatgtcccg cccgggctgc acttcaagat gcccatcgcc gaggaggtca
     5341 aaaaggccga cggacgcgtg ctgacgctgg actccgtgcc agaaacctac tacacgctgg
     5401 agaagaagcc cctcatcgtc gactccttcg ccaagtggcg catcgtcgac gtgcagcgtt
     5461 actacacggg cacctcgttc gacgagcggc gcggccagcg agtgctccag gagcgggtca
     5521 acgaagggct gcgaaaccag atcagccgtc gcgacatgca cgaggtggtc tccggcgagc
     5581 gcgaccagct catgcaggag ctgctggggg acctcaatgc ggtcatgagc ggagccgtgg
     5641 gcatcgaggt gatcgacgtg cgggtcaagc gcatcgacct ccccccggac gtctcgagct
     5701 ccgtgtacga tcgaatgaac tcggaacggc agatcgaggc gcgccagtat cgcgctcagg
     5761 gtcgcgagct cgcgctcggc attcgtgctg acgctgagcg tcagacggtg atcatcgaag
     5821 ccgaggccta ccggagttcg gagcagatac ggggtgacgg ggacgcgcgc tcggctaaca
     5881 tatatgccag tgcgtacaat cgggatcccg agttctacca gttctatcgc agcatcaatg
     5941 cctacgcgaa ggtgttcaag gacaagagcg acatcctggt gctggatccc acgagcgagt
     6001 tcttcaagta tctcgccaac gattcctaag ccgggctaaa cgttccggct gaaatggtcc
     6061 gctgattgga cgtcagatcc atggtcaacc gaaaatggcg cttgcccgac ggggtggacg
     6121 agctactgcc gcctgaggcg tgggcgctgg agcaggtgcg ccgtcaggtg ctcgacgtgt
     6181 tcaacagctg gggcttcgac tacgtcgagc ctccggtggt cgagtacctc gatgcccttc
     6241 tcgtcggcgc aggtccggat ggggggctcg gtcaccagga cgatctcgat ctgcagacgc
     6301 tgaaggtggt cgaccagcgc agcggacgcc tgatgggcgt tcgcgcagac atgacggccc
     6361 aggcggttcg aatcgacgcc cacagccgtc cgagcgaggg ggtgcagagg ctttgctacg
     6421 cgggccccgt ggttctcgcc aacccggcgg cggcgctgga ttcccgggtt ccgctcaagg
     6481 caggcgccga ggtgttcggc gcgcccgggc tcgcggccga cgtggaagtg atctgcctgc
     6541 tgctggaaat tctcgagcgc acgggcgtcg aagccccggt gctcgtgctc ggacacatgg
     6601 ggatctacct gagtctggtc gcaccgctgg gtctggatgc gttcaccgag cggcagctgt
     6661 tcgctgccgt gcagagcaag tccgaggcag acatcggtga gctgatcggc gagcgctcgg
     6721 acggggcgct tttagagcgg ctgccgaccc tcatgggcgg ccgcgagata ctccaggagg
     6781 cgcgcgcggc gttggctggc gggccggatg gcgcggtggc cgcggtggat gctctggagg
     6841 agctggcgga ggcggtgctg gtccgcaacc cggcggtgga agtgcgctac gatctggcgg
     6901 agctcgccgg gtacggttat cacaacgggc cggtgttctc cgcctaccag gcggatcagg
     6961 gccgcgcgat cgccaggggc ggacgctacg acggaatcgg cgcgccgttc ggccgcagcc
     7021 ggcccgcgac cggcttcgac gtgaacctgc gccagctcat cgggcttgcc ggggcaggga
     7081 ccgtcggcca ggccgtctgg gcgccctgga ccggcagcct cggcggcgat gtcgctgccg
     7141 cgctggctgc ggaggtccgg cgcctgcgtg cggctggaga gagggtggtg gtggcgctgg
     7201 acgacgaaga cggggcgcct tcgggctgcg atcgtcagct ttcgtacgtc tctggggtat
     7261 ggaaagtgga aaggctcggg cggaacgagg gctgatatgg gccgcaacgt agtcgtcatg
     7321 ggcacccagt ggggggacga gggcaaaggc aagatcgtcg atctcctcac cgatcaggta
     7381 gcggccgtgg tccgtttcca gggtggtcac aacgccggcc acaccctggt catagagggc
     7441 gagaagacgg tcctgcacct gattccctcg ggcattctgc gtcgcgaggt gcagtgcttc
     7501 atcggtaacg gcgtcgtcct ggagcccaac gcgctgctca gcgaggtgtc cggtctcgag
     7561 gcccgtggcg tgcccgtgcg cgagcggctc cggataagcc ccgcctgccc gctcatcctg
     7621 ccgtaccacg tcaagctcga tctcgcccgg gaggaagcca ggggtaatca gaagatcggc
     7681 acgaccgggc gcggcatagg ccctgcctac gaggacaaag cggcgcgacg cggcgttcgt
     7741 cttggcgatc ttttctactg gcaggagttc gcggccaagc tggccgaggt catggagtat
     7801 cacaacttca tgctggagcg gtactacggc tcggagaccg tggactacgc gcagaccctg
     7861 gatgagtgcg cggcggttgc cgagcagatt cgtcccatgg tggcggacat cattccgctg
     7921 ctgcacgaga ttcgcgaaag cggagccaat ctgctgttcg agggagccca gggcgcgctc
     7981 ctcgacattg atctgggcac gtatccgttc gtgacctcct ccaacacgac cgcgggcggg
     8041 accgcggtgg gcagtggctt cggacctcga tatctggatt acatcctggg catcacgaag
     8101 gcatattcca cccgcgtggg ctcgggaccg tttccgacgg aactcttcga tgaggttggc
     8161 gagcggcttg cgacccgcgg caacgagttc gggtccacca cgggcaggcc gcgccggtgc
     8221 ggctggttcg atgccgtggc cctgcggcag gccgtgcaga tcaacagcat ctccgggctc
     8281 tgcctgacca agctggacgt gctcgacggt ctggagagta tctgcatctg cgtgggttac
     8341 gagggcccgg acggcacgag cggcacgccg cggtttgcca gcgagtatta ctcggaaatc
     8401 aggcccgtgt acgaagaggt ggacggctgg tcggagtcca ccctgggcat ccggcgcctg
     8461 gatgacctgc ctgccaatgc gcgggcgtat ctggcccgca tcgaggagac ggtgggtgct
     8521 cccatcgaca tcatctcgac gggtccggac cggaccgaaa ccattgttct gaaggatccg
     8581 ttcactgcct gagatcggtg gaggcgcaac gtcatgtcga ttgactcgaa gctgctcgaa
     8641 ctgctggtct gccccgtcac caaggcgtcg ctgacctaca ataaagacaa ggacgagcta
     8701 tggtgccgcg ccagcggcct cgcctacccc atccgagacg gcatccccgt gatgctcgag
     8761 gaggaagccc gcgagctcag cgccgaagag cgggaaagcc tgagggagtg atgctccggc
     8821 ccccggaacc gggctccgcg ccggccggcg gcaccttctg tgccgttgca ggccgattgg
     8881 gtacaatccg gggccgcccg tttcacgggg gggggtagct ggaggaagaa cacatgcagg
     8941 tagacccatg aacaagggcg agcttcgcag cgaaaaggaa ctgcccgatg tggccagtca
     9001 cgagctgcat cagccgcacg tgaccctgga ttgggtcggc atggccgaca tccatcagcc
     9061 tcttctcgtg cgtgatggaa accagaccag gcacgtccag tcccgtgttc agatctacgt
     9121 ggacctgggt gacgagctgg ccaagggcat tcacatgtcg cgcctctacc tgatcctcga
     9181 cgagcatgcc gagacccggc ccttgagcgc ggcgggcttg aagctgctgc tgcgcagcgt
     9241 gctcgagtcc catcgcgagc tgagctcccg ggcatacgtg cagttcgatt tcgattacta
     9301 cctgcgccgg caggctctgg tgagcgacaa ctccggctgg aattcgtatc cggtgtccat
     9361 caggggcacg ctgatcgacg gagagattac cctggaggtc gccctcaacg tgcagtactc
     9421 gtcgacgtgc ccgtgctccg cggccctggc gcgtcagctg attcaggatc agttcgacga
     9481 ggacttcggc acctccggga gcgtggccgt gggcgatgtt cgtgcctggc tgggcaccga
     9541 gcagggcatc gtggcgacgc cgcacagcca gcgcagcgac gcgcgaattc gcgtcaagct
     9601 gcgcgaaggc ctcatcgact ttcccataac cgatctgatc gacgaggtgg aagccacgct
     9661 gaagacgccg gtgcagaccg ccgtcaagcg ggaggacgag caggagttcg ccaggctcaa
     9721 cggccagaat ctcatgttca tcgaggatgc cggacgcaag ctcaaggatc agctggatgc
     9781 ggacgaccgt ctcgcggact tctgggtgcg cgtggagcac cacgagagcc tccatgccca
     9841 cgacgccgtg ggggtgttca cgaagggtgt cggggacgga taccaggcag ttccctaggt
     9901 gtcgaaaccc gtcgtcatca gtggcgccgg gctgtggaag cccgaccacg tcatcaccaa
     9961 tgcagaactc gtcgccgcct acaacgcgta cgcggacaga ttcaatgcgg agaacgacgc
    10021 tgccatcggg gctggcgacg tcgcgccgat ggagcactcg agcgaggagt tcatcgagaa
    10081 ggcctctggt atcaagcagc gttacgccta cgtgaaggac gggatcctgg aggtggggcg
    10141 aatgcgtccg cgcattcctc accgcgagga ggacgagctt tcccaccagg ctgaaatcgc
    10201 tctccacgcc gcccggggcg ccatggctgc ggcaggtaag gcggcgtcgg acatcgacat
    10261 ggtcatcgtc tcctgcgcct acactgagcg ctcctaccct gctatcgcca tcgaggttca
    10321 gcacgccctg gggattgacg gctttgcctt cgatatgctc gtcgcgtgct cagcggcgac
    10381 gtttgcgctg cagcgtgctc atgacgccat agcggccggg tccgcgcgat gcgtattggt
    10441 ggtgaacccg gaactcacct cgcctcaggt gaactatcgc gacagggacg gccacttcat
    10501 cttcggtgac gtgagcacgg cggttgtgct cgagtccgca gaaacctgca ccgccgcgca
    10561 cgcgtacgaa attctcggca gcaaagcgct gaccagctac tcgaacaaca tccggtccaa
    10621 tttcggctac ctggcatatg ccgaggacgt ggaccccttc tgcgccgcca acctctttca
    10681 ccaggaaggc cgtcgtgtgt tcaaggaggt ctgccccatg gcggccgagc acctgaaatc
    10741 ccaggtggag gcggtgggac tggacgtggc cgatgtgaaa cgctggtggt tgcatcaggc
    10801 caatctgaac atgaacctgc tgatcagcaa gcggctgctt ggccgcgatg cctcggcgaa
    10861 ggaagcgccc atcgtgctgg acgagtatgc caacacggcg tcggcgggct cgatcatcgc
    10921 gttctgtctg catcacgagg acctcgccgc cggagaccat ggcgtgatct gctcgtttgg
    10981 tgcgggttac tcgatcggca gcctggtgtt gagaaagcgg gcggcggcgt gatgtgaggg
    11041 cgcgccgctc aaagagaatt cctgtttcgg ggtcgccggg cacgctctaa aatgtggtgc
    11101 cgaggagagg acttgaacct ccacggggtt gcccccacta gcacctgaag ctagcgtgtc
    11161 taccaatttc accacctcgg cacgaggggg gcgcaattta ctgaagtgcc ccgctccttg
    11221 tcaaacgatg gcgacgcgaa cgaaaaagac gaacagttcc tccaagatca ccgcccgcga
    11281 tgtccgggaa tccctgggcc gcggccgggg tccgctcgcc tggcgggagc tggtggcggc
    11341 caccgtcggc gactcggcat ccggccgccg ggagcttcgc cgcgtgctgc gtgggctcgt
    11401 cagcgccggc gaggtggtgc agatacccga tgggcgctac cagctcgagg agcccgctgg
    11461 cgtggaaagg ggtgtcgtgg ctctgcaccg cggccggctg tgcgtgggcg agattccctt
    11521 ggagacgggc cgccgggttc gcctcagacc gggcgacgtc gtcgagatgc agatcggtca
    11581 gggggcggac ggcgacatcg cccgtgtcct gcgcgttatc gagtactcgg cggaaccgat
    11641 catcggccag ctcaacgggc gcgggcgaca tccctatgtc gactccctga gcccgcacta
    11701 caaggggcgc gtctccctgg tcgatccgcc tgcttttggc aaccacggcg acaccgtctc
    11761 ggtgcgcatc gtcgatgagg atcgacgtgg actggtggga tatgtcgagg cgctcgtttc
    11821 ggatcgccgc ggcgccgccc acgccgcgga aaccatgctt gccagccacg gtgtgcccag
    11881 acagtggccg tcggcggtgc tcagtgccgc ccagaggctg cccaaggcgg tggttccggc
    11941 ccgtcatccg gatcggcgcg acctggccgg tctgccgctg gtgaccatcg atggtgagac
    12001 cgcgcgggat ttcgacgatg cggtatacgc cgcccggcgt cgcggcgggt ggcgtctggt
    12061 cgtcgcgatc gccgatgttg ctcactacgt gaagccgaaa ggtgcgctgg acgcggaggc
    12121 gctggagcgg ggcaactcgg tttacctgcc cgaccgggtg gtgccgatgc tgccggaagc
    12181 gatctccaac gggctctgct cgctgaaacc tcgagagccc cggcttgcca tggtttgcga
    12241 catgcaggtt tcgaacgcgg gcaacgtcac gcgtttcgag ttctacgaag cggttatccg
    12301 ctcctggcag cggctcacct acaagcgggt acaggaattc ctggattccg gcgcgctgga
    12361 cgtggagccc gaggtctgcc ggtcgcttgc cgacctgcag gggacgttca gcgccctgcg
    12421 ggtggcccga gatcaacgcg gggcgctgga tttcgacact cacgaggcga cgctggagct
    12481 ttccggagat cacgttgcgg ccattcaccc cttcatccgc acggacgccc accggctcat
    12541 cgaggaagcc atgatcgccg ccaacgtgtg cgcggcgacg tttgtcgaaa agcacggagt
    12601 ctccgctctg tatcgggtgc acgaagggcc gtcgggagaa agggaccagc gggtacggca
    12661 ggcgttcgcc tacgccggga ttcgcctgcc gcgggggcgg cttgatccgg cgatgctgcg
    12721 tcaggccctg gatggcctgg gggaacggga cgaccgctgg gtgttcgaga tgctcgtgct
    12781 gcggtcgctg acccaggccg tctaccgccc cgagaacaag gggcacttcg gcctcgcgct
    12841 gacgcggtac atgcatttca cctctcccat ccgacgctat gcggacctgg tggttcacag
    12901 ggcgatcaag gccgcgctcc atggcgcccc gcccccggca gcggacgatt ggctggtggc
    12961 ctgcggcgag caggtctcca tgacggaacg gcgtgcggac gaggtcagct gggccgtgga
    13021 cggatggctg aaatgcgagt acgtctcctc gcgtatcggc gaggtgttcc ccggcgtggt
    13081 catgggtgtg accgatttcg gtcttttcgt ggagctgacc ggctactacg tgcagggatt
    13141 gctgcacgtg tcggatctgg gaagcgacta ctttcagtat cagccggcga cgctggcgct
    13201 ggtgggcgag cgctcgaacc ggcgtttcgg tctgggcgac cacctcaccg tccggctcgc
    13261 gggcgtgcag ccggccctgg gccggctcga tctcgagctt gtcgagcccg ctgccggctc
    13321 tgccagtccg cggacgaacc gacgccgccc cgccgctgac cgtgagactg gccgcggcgg
    13381 cgcccgcggc ggcgcccgcg gcggtccccg aggcaggaag cgtcgatgac ggatggttcc
    13441 aaggtacacg ggatccatgc cgtgaccgcc gtgctgcgcg acgacccggc gcgggtccgg
    13501 gtggtttact gcatcaccgg tcgcgtcgat gcccggatgc gcgaggtgct ggaggaagcc
    13561 cgcgcagccg gggtacgggt cgagcgggtc gaccgacgtc agctcgatcg cctggcaggc
    13621 tcgggccacc agggcgtcgc cgcgcactgc cacgagctgg ccctggccga cgagcgtgcg
    13681 tttgccgagc atctcgacac ccttcccgag ccttggctca tgctggcgct cgacggggtc
    13741 acggatccgc gcaatctcgg cgcctgcctg cg
//
LOCUS       DBNHOX010000361         8457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_992985, whole genome shotgun sequence.
ACCESSION   DBNHOX010000361 DBNHOX010000000
VERSION     DBNHOX010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8457
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_992985"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="AC16UZ_07935"
     CDS             <1..439
                     /locus_tag="AC16UZ_07935"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6246651.1"
                     /translation="QRALRADPSLTRTFVEEALRLEAPVQGLPRLVTKDTELGGVELE
                     AGKLIMIRYGAANRDGRQFENPDELDLHRKKAGMQLAFGSGVHHCIGAPLARQELNLG
                     FPTLLRHMSEIRLSADHPPPEAEASFILRNLPELHIDYDLIEP"
     gene            461..1483
                     /locus_tag="AC16UZ_07940"
     CDS             461..1483
                     /locus_tag="AC16UZ_07940"
                     /inference="COORDINATES: protein motif:HMM:NF028222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6246652.1"
                     /translation="MKAALMENGRIWVDTVPDPEPRLGEVLVKSAGCGICGSDLHAYR
                     HTEEFVATSRESGGAFRLTTMNPVVLGHEFCAEVVDYGPGTQRRLPVGTLVCAIPALP
                     RGGGFEPIGYSEDAPGGFAEYFLLNERLMMPVPPGLSWELAALTEPMAVGHHAINKAR
                     LDGEAVLVIGAGPVGLAVIANLKQRGVGPVVVAELSPGRRALAERLGADVVVDPAAGS
                     PYAVPEVSRAKGLVIFECVGVPGMIGQIFLDAPREAKIVVVGVCLQQDVSRPLIAINK
                     ELNVQYVLGYSIDEFTESLNMIADGTLDVEAMITHRIALEDVPRAFDDLGSPEVQGKV
                     LVQPWA"
     gene            1584..1904
                     /locus_tag="AC16UZ_07945"
     CDS             1584..1904
                     /locus_tag="AC16UZ_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR6246653.1"
                     /translation="MPKITFITHDGVEHVVDASNGASVMNAAIDNLVPGIDADCGGEC
                     SCATCHVYVDPSWTDVVGGPGDREEEMLDLNPERESNSRLSCQIPVRDELEGLVVRVP
                     EFQM"
     gene            1916..3163
                     /locus_tag="AC16UZ_07950"
     CDS             1916..3163
                     /locus_tag="AC16UZ_07950"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6246654.1"
                     /translation="MSEAIYENPNVLEQDPWQLPLEDINPAQAELFENDCHWAYFERL
                     RRDNPVHYCRDSIFGPYWSVTKYADIKYVDTNHEIFSSEPTIVLDDQDEDFPLPMFIA
                     MDPPKHDRQRKEVAGVAGPRNLAALEPTIRQRVCSILDSLPVREKFNWVDLVSIELTT
                     QMLATLFDFPFEDRRKLTRWSDVATAPIGVLVDTDEARQAELLECLEYFTGLWNERVN
                     QPPGNDLVSMLAHGESTKNMEPMEYLGNLVLLIVGGNDTTRNSISGGVLALNQNPDQY
                     EKLRDDLSLIPNMCSEIIRWQTPLAYMRRVAVEDTDLGGQRIKAGDKVAMWYVSGNRD
                     PDMFDDPDAFIIDRANARKHIAFGFGIHRCMGNRVAEMQLRILWEEILRRFHTVELVG
                     EPVRVKSSFVKGYLDLPVILHPN"
     gene            3243..3692
                     /locus_tag="AC16UZ_07955"
     CDS             3243..3692
                     /locus_tag="AC16UZ_07955"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6246655.1"
                     /translation="MSEPDTSCSLVVSRELFVPRELVFDVWTHADHLENWYSPGPDFD
                     RHAEVDAREGGAYLLTWTGLDGRAYGQRGRFDSFHVPDKLSYTSIFEVDGEERISTRT
                     HVSFADLGGGTRIDIVVDGYPDGGSRDAHQASWALFLDQLEAYFSVI"
     gene            complement(3705..4034)
                     /locus_tag="AC16UZ_07960"
     CDS             complement(3705..4034)
                     /locus_tag="AC16UZ_07960"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6246656.1"
                     /translation="MNTSAWQVFHVDDLKSKLKGGAVEYKEFLRVPSVSCGLYHLAAG
                     SQDMQTPHDEDEMYFVVDGRARLKVGDEIYEVRPGSVLYVQATEEHSFFEIEEDMTLL
                     VFFASTS"
     gene            complement(4093..5598)
                     /locus_tag="AC16UZ_07965"
     CDS             complement(4093..5598)
                     /locus_tag="AC16UZ_07965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6246657.1"
                     /translation="MGQSIAIVGSGICGMCTAMTLAGQGHDITVFDKDVSPPAGSAEE
                     AFFSWERRGAAQFRHPHAFLGLMCNLIEERYPELLDAFYAAGARRVDLQEMLPAELRE
                     SYRPVRGDEKLWVLLCRRATIETIIRQYVGALPGIRIVNPCKVTDLITDSEGGRLQVK
                     GLMIQREHEGEVTPFQADVVIDASGRRTRFPRWFAQRGCTIPEEKHDAELVYYTRHYR
                     LKEGRSEPARSDRSGAGDLGYLKFGVFPGDNGHFAIILCVPTPETAIRKAVRSGDLFD
                     RICLSIPGLEPWLADGRAEATTDPFGIGDINAVWRNFVRDGQPLAANFFAIGDAAVRT
                     NPLYGRGCSTGLLHASILTDVLAETSDPVERALAFHQRTVSELRPIYDASLGEDRRGI
                     KRATASMAGGLLEPPRTLRRWFALAFGDALSAASREQAHVLRGALRSFHLLEKPGEFL
                     NDWRIRRTVLRYMLRGRKRNATMRLQPGPERVELHELLGLQAPEPTAEPAP"
     gene            complement(5611..6492)
                     /locus_tag="AC16UZ_07970"
     CDS             complement(5611..6492)
                     /locus_tag="AC16UZ_07970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6246658.1"
                     /translation="MIEKIPFGRTGHESTRTLFGAAALGSMSPERAEGVLATLLEHGV
                     NHIDTAASYGDSELRLAPWLAANRASFFLATKTDQRTGSAARAQLERSLERLGVDQVD
                     LIQMHNLVDEQEWETAMAPGGALEALIDARAEGLVRFIGVTGHGTRVAAMHLKSLEQF
                     DFDSVLLPYNFTMMQQSDYAADVEALLKICKSRNVAVQTIKSVARRRWTDDDADKRFS
                     WYMPLRDRESLQRAVHFVLSRPGLFLNTSSDATILPLILEAASQPAEAPDPDAMRCDV
                     NALGMEPLFVRGVSDTI"
     gene            complement(6566..7393)
                     /gene="larE"
                     /locus_tag="AC16UZ_07975"
     CDS             complement(6566..7393)
                     /gene="larE"
                     /locus_tag="AC16UZ_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HIR6246659.1"
                     /translation="MDAKLGAKYEKLKRRIAAFDSAIVAFSGGVDSSLVAYVTAEVLG
                     PRALAVTSGSKSLKRSDLHLAVELADKWGMQHRVIVTDELSKPDYRANPIDRCFHCKS
                     SLYAALTAIAREEGYAMIFSGTNCDDLGDHRPGLKAADDHGVTAPLVEAGFGKADIRA
                     LADELGLDNAQKPQSACLSSRFPYGTAIDENRLAQVEQAEDVLASFGFTQFRIRHHGD
                     VARLELLPDEIPAALDRAADIESAIRACGYRFVAVDLRGFRSGSLNEGMVKVVNLPA"
     gene            complement(7404..8156)
                     /gene="larB"
                     /locus_tag="AC16UZ_07980"
     CDS             complement(7404..8156)
                     /gene="larB"
                     /locus_tag="AC16UZ_07980"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HIR6246660.1"
                     /translation="MSAQHDELAALLEAYREGTVALDEALSGIRAAFFQDLQHSTIDH
                     DRRRRTGAAEVIYGQHKTPQQIADTFAALAETGGDVLATRVSAEAYELLEARVPHARY
                     HRDAELVMLKQSEPDPAPTKIAVVTAGTSDYRVAEEAALTAEFYDSPVIRISDVGVAG
                     LHRLLSRIDAIRAARVVVVVAGMEGALPSVIGGLVDKPVIAVPTSVGYGAAFDGVAAL
                     LGMLTSCASGVGVVNIDNGFGAGFLANMINRL"
     gene            complement(8153..>8457)
                     /gene="larC2"
                     /locus_tag="AC16UZ_07985"
     CDS             complement(8153..>8457)
                     /gene="larC2"
                     /locus_tag="AC16UZ_07985"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC2"
                     /protein_id="HIR6246661.1"
                     /translation="VTVLVGEERFDATLEALFAGSTTIGARVHRVEKRMLPREARRMT
                     TSLGEVRIKLVTRPDGRRTWKVEHDDLVALAERHAMSYLDVRHMIEVEARRELGDS"
BASE COUNT         1519 a         2651 c         2703 g         1584 t
ORIGIN      
        1 ccagcgggcg ctgcgcgccg atccgtcact cacgcggacc ttcgtggagg aggccctgcg
       61 gctggaagca cccgtccagg ggctcccgcg cctggtgacg aaggataccg aactcggcgg
      121 cgtcgagctg gaggcaggca agctgatcat gatccgctac ggtgcggcca atcgcgacgg
      181 tcgtcagttc gagaatccgg acgagctcga cctgcaccgc aagaaagcgg gcatgcagct
      241 ggcgttcggt tccggggttc atcactgcat cggcgccccg ctggcccggc aggagctgaa
      301 tctgggtttt ccgaccctgc tacggcacat gagcgagatt cgtctatcgg ccgatcatcc
      361 gcccccggag gcggaggcca gcttcattct ccgcaacctg ccggagctgc acatcgacta
      421 cgacttgatc gagccctgat cgccgagacg ccggatcatc atgaaagcgg cgctcatgga
      481 aaatggtcgg atctgggtag acaccgtccc cgatcccgag cctcgactcg gagaggtgct
      541 ggtcaagtcc gcgggttgcg ggatatgcgg atctgacctc catgcctacc gtcacacgga
      601 ggagttcgtg gcgaccagcc gcgagtccgg gggcgctttc cgcctgacca ccatgaatcc
      661 ggtggtcctg ggccacgagt tctgcgctga ggtcgttgac tacggacccg ggacgcagcg
      721 ccgccttccc gttggcacct tggtctgtgc cattcccgcg cttccgcggg gcggcggttt
      781 cgagcccatc ggttactcgg aggacgcgcc gggggggttc gccgaatact ttctgctcaa
      841 cgagcgattg atgatgcccg taccccctgg gctttcctgg gagcttgccg cgctgacgga
      901 gcccatggcc gtcggccacc acgccatcaa caaagcaagg ctcgatggcg aagcggttct
      961 cgtcatcggc gccggtcccg taggtcttgc agtgatcgca aacctcaagc agaggggcgt
     1021 cgggccggtg gtggtcgccg agctctcacc gggtcggcga gctctggccg agcgtctggg
     1081 ggccgacgtc gtggtggatc ccgccgcggg ctcgccttac gcggtcccgg aggtctcccg
     1141 cgcgaagggc ctggtgatct tcgagtgcgt gggcgtgccg ggcatgatcg ggcagatatt
     1201 cctcgacgct ccgcgggaag ccaagatcgt ggtggtaggc gtctgccttc agcaggacgt
     1261 cagtcgtccc ctgatcgcaa tcaacaagga gctgaacgtt cagtacgtgc ttgggtacag
     1321 cattgacgag ttcacggaat ccctgaacat gatcgccgac ggcacgctgg acgtggaggc
     1381 gatgatcacg caccggatcg ctctggagga tgtgcctcgg gccttcgacg atctgggcag
     1441 tccggaagtc cagggcaaag tgctcgttca gccctgggcc tgagctcgat gcctgcgaac
     1501 taggggtaaa ccgaggctgt tatcccgacg ggattctagc tatcgttgcg gtttttccac
     1561 tcggagcacc tagagggaga cgtatgccca agatcacctt catcacccac gacggcgtcg
     1621 agcacgtcgt cgacgccagc aacggggcgt ctgtcatgaa tgcagccatc gacaatctcg
     1681 tccccggcat cgatgcggac tgcgggggtg agtgctcctg cgcgacgtgt cacgtctatg
     1741 tcgacccgag ctggacggac gtggtcggcg gccccggcga tcgcgaggaa gaaatgctcg
     1801 atctcaatcc cgagcgcgag tccaactcca gactgtcttg tcagatcccg gtccgcgacg
     1861 agctcgaagg gctggtcgtc cgggtacccg agtttcagat gtaaaacggt ggcgtatgag
     1921 tgaagcaatc tacgaaaatc caaacgttct agagcaggac ccgtggcagc tccccctgga
     1981 ggacatcaat cccgcccagg ctgagctctt cgagaacgac tgccactggg catattttga
     2041 gcggcttcgc cgcgataacc ccgttcatta ctgccgggac agtattttcg gaccttactg
     2101 gtccgtgacg aagtacgccg acatcaagta cgtcgatacg aatcacgaga tcttttcctc
     2161 ggagcctacg atcgttctgg acgatcagga cgaggacttt ccgctgccca tgttcatcgc
     2221 catggatccg ccgaagcacg atcgccagcg aaaagaagtt gccggggttg cggggccgcg
     2281 aaaccttgct gctctggaac cgaccatccg ccaacgcgtg tgctcgatcc tcgattccct
     2341 gccggtacgc gaaaagttca actgggtgga cctggtctcc atcgagctga cgacccagat
     2401 gctggcaacg ttgtttgatt ttccgttcga ggatcgacgc aagctgaccc gctggtcgga
     2461 tgtggcgact gcccccatcg gggttctggt ggataccgac gaggcccgcc aggccgagtt
     2521 gctcgaatgc ctggagtatt tcacgggatt gtggaacgag cgggtcaacc agccgcccgg
     2581 caacgatctg gtctcgatgc tcgcccatgg agagtccacc aagaacatgg agccgatgga
     2641 gtacctcggc aacctggtcc tgctgatcgt cggaggcaac gacacgacgc gaaactccat
     2701 cagcggcggc gtgctggctc tcaatcagaa tcctgatcag tacgagaaac tgcgcgacga
     2761 cttgagcctc attccgaaca tgtgctccga gatcattcgc tggcagacgc cgctggccta
     2821 tatgcgtcga gttgccgttg aagatacgga ccttggcggc cagcggatca aggcgggaga
     2881 caaggtggcc atgtggtacg tgtcgggcaa ccgcgacccg gacatgttcg acgacccgga
     2941 tgccttcatc atcgatcggg cgaacgcgcg caagcacatc gcgttcggtt tcggcatcca
     3001 tcggtgcatg ggcaaccggg tggcggagat gcagctgcgc attctctggg aagagatact
     3061 gcgccggttc cacaccgtcg agctcgtggg cgagccggtt agggtcaagt cgtcgttcgt
     3121 caaggggtat ctcgacctgc cggtgattct ccaccccaat tgagagcccg ttccccagga
     3181 gagcacggtc gccgacttcg aaaagttgcc aaggattgca ggtgattgag gacgacagcc
     3241 agatgagtga acccgacacg tcctgctcgc tggtcgttag ccgtgagctc ttcgtgcccc
     3301 gcgagctcgt tttcgacgtc tggacccatg ccgatcacct ggagaactgg tactcgccgg
     3361 gccccgattt cgacaggcat gccgaggtcg acgcccgtga gggcggcgct tatctgctga
     3421 cctggaccgg gttggacggg cgcgcttacg ggcaacgagg ccgtttcgat agctttcacg
     3481 tcccggacaa gctcagttac acctcgatat tcgaagtcga cggcgaggag cggatcagca
     3541 cgcggaccca cgtcagcttt gccgaccttg gtggcggtac ccggatcgac atcgtggtcg
     3601 atggctaccc ggacggcggc tcgagagacg cgcaccaggc aagctgggcg cttttcctcg
     3661 atcagctgga ggcgtacttt tcagtcatct gaggcccgcc gggatcagct ggtggaggcg
     3721 aagaagacca aaagggtcat gtcctcctca atctcgaaaa atgaatgttc ctccgtcgcc
     3781 tgaacgtaga ggacggatcc cggccgcacc tcgtatatct cgtcgcctac cttcagccgg
     3841 gctcggccgt cgacgacgaa gtacatctcg tcctcgtcgt gaggggtctg catgtcctga
     3901 gagcctgcgg ccaggtgata cagaccacaa gagaccgagg gcacccgcag aaactctttg
     3961 tactccaccg cgccgccctt gagtttcgac ttgagatcgt cgacgtgaaa gacctgccag
     4021 gcgctcgtgt tcatgtttcc cccgttgaag gcattccgga tgctcgaaga cttcagggca
     4081 attgtacagg gtttacggtg caggttcggc tgtgggctcg ggggcctgca aaccgagcag
     4141 ctcgtggagc tcgacacgct ccggtccagg ctgcagacgc atggtcgcgt tacgcttgcg
     4201 cccgcgcagc atgtagcgca gcacggtgcg ccggatacgc cagtcgttca ggaactctcc
     4261 gggcttttcg agcagatgga acgaacgcaa ggcgccgcgc agcacgtggg cctgctcccg
     4321 ggaagcggcg ctcagcgcgt cgccgaatgc cagcgcgaac cagcggcgca aggtccgcgg
     4381 cggttcgagc agcccgcccg ccatgctcgc cgttgcgcgt ttgatgccgc ggcgatcctc
     4441 gcccaggcta gcgtcgtaaa tgggccttag ctcgctcacc gtgcgttgat gaaaggccag
     4501 cgctcgctcg accgggtctg atgtctcggc gagcacgtca gtaagaatgc tggcatgcag
     4561 gagcccggtg ctgcatccgc gtccgtacaa agggttcgtg cgcacggcgg cgtcgccaat
     4621 ggcgaaaaaa ttcgcggcca gcggttggcc gtcgcggacg aagtttcgcc atacggcgtt
     4681 gatgtcgccg atgccgaatg gatcggtggt ggcctctgcg cggccatcgg ccagccaggg
     4741 ttcgaggcct ggaatgctca agcagatcct gtcgaacagg tcgccgctgc ggacggcctt
     4801 tctgatggcg gtctcgggcg tgggcacgca cagaatgata gcgaagtggc cgttgtcgcc
     4861 agggaacacg ccgaacttga ggtaccccag atcgcccgcc ccggaacggt cgcttcgggc
     4921 aggctccgat cggccctcct tcaggcggta atgccgcgtg tagtagacga gctccgcatc
     4981 atgtttttcc tcggggatgg tgcaaccccg ttgtgcgaac caccgcggaa agcgggttcg
     5041 ccgtccgctc gcgtcgatga cgacgtctgc ttgaaacggt gtcacctcgc cttcgtgctc
     5101 acgctgaatc atgagcccct taacctgcag acggccccct tccgagtctg taatgaggtc
     5161 cgtgacctta cagggattca cgatcctgat cccgggaaga gcgcccacgt actggcgaat
     5221 gatggtctct atggtggctc ggcgacagag caggacccag agcttctcgt cacccctcac
     5281 gggtcggtaa ctctctcgca gctccgccgg gagcatctcc tgtaaatcga cccgtctcgc
     5341 tccagcagcg taaaaggcat cgagaagctc agggtagcgc tcctcgatca ggttgcacat
     5401 caggcccaga aacgcatgcg ggtgtcggaa ctgcgcggcg ccccgacgct cccagctgaa
     5461 aaaagcctcc tctgcgctgc ctgcaggtgg cgagacgtcc ttgtcgaaca cggtgatgtc
     5521 gtgcccttgc cccgcgagcg tcatggccgt gcacatcccg cagatgcccg aaccgacgat
     5581 ggcgatgctc tggcccacag ggttgttgcg tcagatcgtg tcagagacgc cgcggacgaa
     5641 cagcggttcc atccctaggg cgttcacgtc gcaacgcatg gcgtccggat ccggcgcctc
     5701 ggcaggctgg ctcgcggcct ccaggatcag cggaagtatg gtcgcgtcgc tggaggtgtt
     5761 gaggaagagg ccggggcggg agaggacgaa gtgcacggct cgctgaaggc tttcccggtc
     5821 gcgcaggggc atgtaccagc tgaaacgctt gtccgcgtcg tcgtccgtcc accgccggcg
     5881 cgccaccgac ttgatcgtct gcaccgcgac gtttcgggat ttgcagatct tcagcagcgc
     5941 ttccacgtct gccgcgtagt cagattgctg catcatcgtg aagttgtagg gcaggagcac
     6001 ggagtcgaag tcgaactgct cgaggctttt cagatgcatc gcggcgacgc gggtaccgtg
     6061 accggtgacg ccgatgaagc ggaccaggcc ttcggctctg gcgtctatca gtgcctccag
     6121 ggcgcccccg ggggccattg cggtttccca ctcctgctcg tcgaccagat tgtgcatctg
     6181 aatgagatcg acctgatcca cgcctaagcg ctccagcgac ctttcgagct gtgcacgggc
     6241 tgcggagccc gtccgctggt cggttttggt ggcgagaaag aagctcgccc gatttgctgc
     6301 cagccagggc gccagccgca gctcggaatc gccgtagctt gccgccgtgt cgatgtgatt
     6361 gaccccatgc tccagcaggg tggccaggac gccctcggcg cgctccgggc tcatgcttcc
     6421 cagcgcggcc gcgccgaata gggtcctggt gctctcgtgg cccgtgcggc caaaaggaat
     6481 tttctcgatc atgtcgatcc gctgcttgct cgagtgactg taatcatggc ccaacggtcc
     6541 aggacggtgc aacgagccct ccgggtcagg cgggcaggtt gaccaccttg accatgcctt
     6601 cgttcagaga tccggaccgg aaacctcgca ggtccacggc aacgaaccgg tagccgcagg
     6661 cgcgaatggc cgactcgatg tccgccgcac gatcgagcgc ggcgggtatc tcgtcgggca
     6721 gcagttcgag ccgcgccacg tccccgtgat gccggatacg gaactgcgta aaaccgaaag
     6781 acgccagcac atcctcggcc tgttcgacct gtgccaatcg gttctcgtcg atggccgttc
     6841 cgtagggaaa acgactcgaa aggcaggcgg attggggttt ctgagcattg tccaatccga
     6901 gctcgtcggc taaagcccgg atatcggcct ttccgaaacc ggcctcgacc agcggggcgg
     6961 tcacgccgtg atcgtctgcc gctttgaggc ccggccggtg atcgcccaga tcgtcgcaat
     7021 tcgtcccgct gaagatcatg gcgtaaccct cttcccgggc gatggccgtc agcgcggcgt
     7081 acagagacga tttgcagtgg aagcagcggt cgatgggatt ggcccgataa tccggcttgc
     7141 tgagctcatc ggtgacgatg acgcgatgct gcatacccca tttgtcggcc agctcgacgg
     7201 caagatgaag gtcgctccgc ttcaggctct ttgagccgga agttaccgcc agcgccctgg
     7261 gccccagaac ttcagcggta acgtacgcga cgagcgagct gtcgacgccg cccgagaacg
     7321 cgacgatcgc gctatcaaac gccgcgattc gacgtttcag cttctcgtat ttcgcaccaa
     7381 gtttggcgtc cacgtgcagt ctctcagagg cggttgatca tgttggcgag gaaacccgcg
     7441 ccaaagccgt tgtcgatgtt aacgacgccg acgccggatg cacagctcgt cagcattccg
     7501 agcagggcgg caaccccgtc gaacgcggcg ccgtaaccga cgctcgtggg cacggcgatc
     7561 acgggtttat ccaccagccc tccgatcacg ctgggcagag cgccctccat ccccgcaacc
     7621 acaacgacca cccgagcggc gcggatggcg tcgatccggc tcagcaacct gtgcaggccg
     7681 gccacgccga cgtcgctgat gcggataacc gggctgtcgt agaactcggc ggtcagcgcg
     7741 gcttcctcgg ccactcggta gtcggacgtg ccggcggtga cgacggcgat cttcgtgggc
     7801 gcggggtcgg gctcgctctg cttcagcatc accagctccg catcacggtg gtagcgtgcg
     7861 tgcggcactc gggcctcgag gagctcgtaa gcctcggcgc tcacccgggt ggcgagaacg
     7921 tcaccgcccg tctcggccag ggcggcgaag gtgtcggcaa tctgctgcgg ggtcttgtgc
     7981 tggccgtata tgacctctgc cgcgccggtt ctacgccgac ggtcatggtc gatggtgctg
     8041 tgctgcaggt cctgaaagaa cgccgcgcga atgcccgaca acgcttcatc cagcgcaacc
     8101 gtgccctcgc gatatgcctc cagcagagcc gcaagctcgt cgtgctgcgc gctcatgagt
     8161 caccgagctc ccgccgggct tcgacctcta tcatatgcct gacgtccagg tagctcatgg
     8221 cgtggcgttc cgccagggca accagatcgt cgtgctcgac tttccaggtc cgacgtccgt
     8281 cggggcgggt gacgagcttg atgcggacct cgcccagaga ggtggtcatc cgccgggcct
     8341 cccgggggag catccgcttc tcgacgcggt gaacgcgggc accgatggtg gtagagccgg
     8401 cgaacagggc ttccaacgtg gcgtcgaagc gctcctcgcc gaccagcacg gtgactt
//
LOCUS       DBNHOX010000362         3341 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_671031, whole genome shotgun sequence.
ACCESSION   DBNHOX010000362 DBNHOX010000000
VERSION     DBNHOX010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_671031"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..1493
                     /locus_tag="AC16UZ_07990"
     CDS             177..1493
                     /locus_tag="AC16UZ_07990"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6246662.1"
                     /translation="MSKSPNHDLSAMRCPAHLEDVDLFSPGAPEHWYEAYDILHAEAP
                     VHRIPGEGFEPGTDAFILSKHEDIALVVRDEERFPVVGSIFLKQVLAGGEEALAQPNV
                     SAVLASMASLRPNPEMWRAHRQELTDPWVGPGAGRNEPMVRRLAGELIDKWIDRGTVE
                     FVSEFAQPLPQMVMASILGWPMEDLGLLKYFGDGTVKPFVYGRRHNNILNDDEIKDQF
                     AVLEEFKQYTANLIQEKRKSPQEDMITFLTQVEYSPLARRLNDIEINGIVYAMVIGGL
                     ETTQYALAEQVQLLIEREAVWDELKADRDKVRAFIEEGMRLRSPTQGLSTRITSQDEV
                     FQGVKVPKGSYLHLRWAAANLDPEEWECPKDLLLDRKAGTRHLVFSQGPRVCPGASLS
                     RVEQMVAWNALLDRVERFSYGENNTFMHQPGIMLGTLELNLEFEKA"
     gene            1593..1668
                     /locus_tag="AC16UZ_07995"
     tRNA            1593..1668
                     /locus_tag="AC16UZ_07995"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1626..1628,aa:Asn,seq:gtt)
     gene            complement(2073..2201)
                     /locus_tag="AC16UZ_08000"
                     /pseudo
     CDS             complement(2073..2201)
                     /locus_tag="AC16UZ_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            2311..>3341
                     /locus_tag="AC16UZ_08005"
     CDS             2311..>3341
                     /locus_tag="AC16UZ_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6246663.1"
                     /translation="MPMISRCAALGAVCVFALHTALAVAEDSSAARYRDSAPGRDWPG
                     YGRTYGEGHYSPLDEIDTGNVGRLGLAWSLELGPENSVTQPIAVDGVLYFATGYSIVQ
                     AVDAKTGKRLWSYDPKAAEAAGINLRLGWGSRGISWWNGKIYTATHDGRLIAIDARDG
                     KPVWTAQTLDRRNALYISAAPRVFDGKVIIGNASDYGAVRGYVTAYDAETGQQLWRFH
                     TVPGDPADGFENDAMAMAAKTWFGEWWKHGGGGTVWNSIAYDPDTDTLFLGTGNGYPW
                     NRRARSAGQGDNLFLCSIVALNGATGDYKWHYQINPGETWDYNAAMDVQLAELPIDGK
                     PRKVVMNAP"
BASE COUNT          611 a         1062 c         1072 g          596 t
ORIGIN      
        1 ctgagacgtg gccgcgatgg cggctcatga gaagcgggca cttggggtgc ccgctttttt
       61 ttggcgcggc cgcggcgcac cgtcgggctc gtccccttcg tgaaacgaat gatgaccacg
      121 cagggaatcc gtaagatcat taacaggctt gcggtcaatc gggaggaggc atcatgatgt
      181 ccaaatcacc caaccacgat ctgagcgcca tgcgctgccc cgcgcacctc gaggacgtcg
      241 atctgttctc gccgggtgcc ccggagcact ggtacgaggc ctacgacatc ctgcatgccg
      301 aggcgcccgt gcaccggatt cccggcgagg ggttcgagcc gggcacggat gctttcatcc
      361 tctccaagca cgaggacatc gcgctggtgg ttcgggacga ggagcgcttt cccgtcgtcg
      421 gcagcatctt cttgaagcag gtgctggcgg gtggggagga ggcgctggcg cagcccaacg
      481 tttccgccgt gctggcctcc atggcctcgc tgcggcccaa tccggagatg tggcgggcgc
      541 accggcagga gctgacggat ccctgggttg gtcccggcgc cggccgcaac gagcccatgg
      601 ttcgccgcct tgcaggggag ctgatcgaca agtggatcga ccgcggcacc gtggagttcg
      661 tctcggagtt tgcccagccg ctgcctcaga tggtgatggc cagcatcctg ggctggccca
      721 tggaggacct gggcctgctc aaatacttcg gcgacgggac cgtcaagccc ttcgtttacg
      781 gccgacgcca taacaacatt ctcaacgacg acgagatcaa ggatcagttt gccgtcctgg
      841 aggagttcaa gcagtacacg gcgaacctga ttcaggagaa gcggaagagc ccacaggagg
      901 acatgatcac cttcctcacc caggtggagt actcgccgct cgcgcgtcgc ctcaacgaca
      961 tcgagatcaa cggcatcgtc tatgccatgg tcatcggtgg tctcgagacc acccagtacg
     1021 cgctggcgga gcaggtgcag ctcctcatcg agcgtgaagc ggtctgggac gagctgaagg
     1081 cggatcggga caaggttcgc gccttcatcg aggagggcat gcgcctgcgc tcaccgaccc
     1141 aggggctctc gacccggatc accagccagg acgaggtgtt ccagggggtg aaggtgccga
     1201 agggctccta tctgcacctg cgctgggcgg cggccaatct cgatccggag gaatgggagt
     1261 gcccgaaaga tctgctcctg gaccgcaagg ccggcacgcg ccacctggtc ttctcccagg
     1321 gccccagagt gtgtcccggc gcgagcctct cccgggtgga gcagatggtg gcctggaacg
     1381 ctctgctgga ccgcgtcgag cggttctcct acggcgagaa caacacgttc atgcaccagc
     1441 cgggcatcat gctggggacg ctggagctca atctggagtt cgagaaggcc tgacgcgcgc
     1501 gccgcggagc acgggctggc ggtatttgca tttgcccagc ggccaattaa actagccgac
     1561 ctcgcgtgga gtgacccacc gcgcgcaccc gttccgcctt agctcagttg gtagagcagc
     1621 tgactgttaa tcagcgggtc gctggttcga gcccagcagg cggagccata tctttcaatg
     1681 atttacacgc ttttcaactg acctcgagat tccccttgtg gggattttgt gggggcgatc
     1741 ctgcgcgtcc cgcgcatgat caccagtcct ggcctcacac cgttccggtc gcagaccgat
     1801 tctgccgcgt cgatgaggcg ttcgagctca ggcgcgaagt agtgcgtggt catacgtgcc
     1861 gacttgtggc ctagcaggtc ctggcggtcc tcaaagctca cccctggcgc acgtagccgt
     1921 cgattggacc cccgaatggt cccgggtggg ccatgtgcca gtcaccgttc ggttccatcc
     1981 tctgatccgc tcctctagac agggcaagtt gggcggattc catgaagacc agtttacgag
     2041 accggacgat gttcaccgcc gcggtccacc acacccagga cctggctcag gatttggggt
     2101 tgcgcggcgc tctctaccat tcgcctggcg ccacacgagg ggcagaatcc gcgtcgcttg
     2161 caggaaaacg ccaccaggcg ctcgtgactg caggactcgc actgaagccc atcacgtagt
     2221 cagctccgcg ctatgcggaa ctccgtttcg ctgttacggt tgaacactca ggacaattct
     2281 ccggcgagtg caaccagaag gccaacatca atgccaatga tctcccgttg cgcggccctc
     2341 ggcgccgtat gcgtgttcgc cctgcatacg gccctcgcgg ttgccgagga ttcctcggcc
     2401 gcgcgttatc gagacagcgc gccggggcgc gactggcccg gctacggccg cacctacggc
     2461 gagggccact acagcccgct cgatgaaatc gataccggca acgtcgggcg gcttgggctc
     2521 gcctggtcgc tcgagctcgg gccagagaat tcggtgacgc aaccgatcgc cgtcgacggt
     2581 gtgctctatt ttgccaccgg ctacagcatc gtgcaggccg tggatgcgaa gaccggcaag
     2641 cgtctctggt cctacgatcc gaaggccgcc gaggcggccg gcatcaacct gcgcctgggc
     2701 tggggaagcc gcggtatctc gtggtggaac ggcaagatat acaccgccac gcacgatggc
     2761 cgcctgatcg ccatcgatgc ccgcgacggc aagccggtgt ggacggcgca gacgctggac
     2821 aggcgcaacg cgctgtacat ttccgccgca ccacgcgttt tcgacggcaa agtcatcatc
     2881 ggcaatgcca gtgactacgg cgcggtgcgc ggctacgtga cggcctacga cgccgagacc
     2941 ggccaacagc tgtggcgttt ccacaccgtg cccggcgatc ccgctgacgg cttcgaaaac
     3001 gatgccatgg cgatggccgc gaagacctgg ttcggcgaat ggtggaagca cggcggcggc
     3061 ggcacggtgt ggaactccat cgcctacgat cccgataccg atacgctgtt tctcggtacc
     3121 ggcaatggct acccctggaa ccgccgcgcg cgtagcgcgg gccagggcga caacctgttc
     3181 ctgtgctcta tcgtcgcgct caatggcgcc actggcgact ataagtggca ctatcagatc
     3241 aatcccggcg aaacctggga ttacaacgcg gcgatggacg tacagctggc cgaactgccg
     3301 atcgacggca agccacgtaa agtggtgatg aatgcgccga a
//
LOCUS       DBNHOX010000363         3703 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_607777, whole genome shotgun sequence.
ACCESSION   DBNHOX010000363 DBNHOX010000000
VERSION     DBNHOX010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_607777"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="AC16UZ_08010"
     CDS             <1..1143
                     /locus_tag="AC16UZ_08010"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6246664.1"
                     /translation="SAVRLLADWGADVIKIEPVPGAGQSVTGARRGPDEQNLHRNKRS
                     LCLDLKHPDGHGLFLELAAVADVAVENFKVDVKHRLRVDYETLKQVNESIIYASISGF
                     GQEGPYADRGGVDQIVQGMSGLMSVTGEPGGGPMRVGVAISDTSAGMFLGQGILLALL
                     HRERTGEGQWVHTSLLEAMMCKLDFQGARYTMNGEVPTQQGNDHPAYTPMGTFLASDG
                     QVNIAASMPRLWQRFCEALGADDLLAHPDYQTTADRIARKDQLKEAINAITSQRTSAD
                     LVGVLNEAGVPCGAINTIGEGFEDPQVRHLGMAKPAPHPDMGSVNLIRSPINLSRFPQ
                     ESAFRNAAPDPGFDTRAVLTELGIPSSRIDALAAAGAIACTDPASA"
     gene            1151..2479
                     /locus_tag="AC16UZ_08015"
     CDS             1151..2479
                     /locus_tag="AC16UZ_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HIR6246665.1"
                     /translation="MNVQPLSVRERMGYGVGDFGVNLFFIPAMTYLLYFYTDVFGLSA
                     AAAGGVFLVARVVDAVTDPLMGGIAERTSSRWGRLRPHILFGALPLGAIAVLTFTTPD
                     LNDAGKLWWAAVTYVLFGIAYTVVSIPYSALTASITADHHERTLLTTVRMGCAFAGGY
                     VVSVGMLPLVGLFETQAAGFAWAMVIFATFATGLLWLTVANTRERVQPPATQKLTVRD
                     SFRAVFANPPLLVVIVLFTGGMLSFTIRQSVTLYYFKYNLGREDLIALFFGITLPVMM
                     VGLLGVPRLAAAVGKAGGIVVGALVTIVGCAGLYLTPYDSVTWVFVWSCVIALGGTPI
                     AVLGWAMIPDTVEYAQWRMGLRADGAIFGFASFFQKLAKAVGGAGVAGALALAGYVAN
                     VEQSEASLRAIHNMMTLAPALIMLVLIIAARAYRLDAATHRRIVQELSGA"
     gene            complement(2495..3199)
                     /locus_tag="AC16UZ_08020"
     CDS             complement(2495..3199)
                     /locus_tag="AC16UZ_08020"
                     /inference="COORDINATES: protein motif:HMM:NF015682.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR6246666.1"
                     /translation="MTELTADLIAELQRLDTPTVCNALEVVAPERRGYGYTVRPLVCT
                     RPELPPLVAVARTATIRAAHPGHLRGSDAQALRDAYYAYVDEGPKPSVMVIQDMDGDQ
                     RGYGSWWGEVNSNVHKGFGCLGVITDGSVRDIPDIAEGFQMLADRIGPSHAYVHPVDF
                     GRTVNVAGMRVNGGDLIHADQHGAVVIPWEVAAEVAAAADTIARRERVIIEAARQPGF
                     DMASLRDAWGDAADIH"
     gene            complement(3397..>3703)
                     /locus_tag="AC16UZ_08025"
     CDS             complement(3397..>3703)
                     /locus_tag="AC16UZ_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6246667.1"
                     /translation="QQSGAELSLCHVYMGYDVDTQQMERELLDSLGELYEVPEDRRYS
                     VPGSVSGAIHRLASRIGADLVVLGTHGRSGPSLAVGSTANAVLHGATCDQLSVRIGA"
BASE COUNT          557 a         1222 c         1259 g          665 t
ORIGIN      
        1 agtgccgtac ggctgctggc ggactggggc gccgacgtga tcaagatcga gcctgtgccg
       61 ggcgcagggc aatcggtgac cggggccagg cgcgggccgg acgagcagaa cctgcaccgc
      121 aacaagcgca gcctctgtct cgatctcaag cacccggatg gccacgggct gtttctcgag
      181 ctcgcagccg tggccgacgt ggcggtcgag aacttcaagg tggacgtcaa gcaccgcctg
      241 agggtggact acgagacgct caagcaggtt aacgaaagca tcatctacgc gagcatctcg
      301 ggcttcggtc aggagggccc ttacgcggac cggggcggcg tggatcagat cgtccagggc
      361 atgagcgggc tcatgtcggt gacgggggag ccgggaggcg gccccatgcg ggtgggcgtg
      421 gccatcagcg acacgtccgc cggcatgttt ctagggcagg gcatcctgct ggcgctgctg
      481 catcgggagc ggacgggcga ggggcagtgg gttcacacgt cgctgctcga agccatgatg
      541 tgcaagctgg attttcaggg cgcgcgctac accatgaacg gcgaggtccc gacccagcag
      601 ggcaacgacc acccggccta cacccccatg ggcacgttcc tggccagcga cggccaggtg
      661 aacatcgctg cctccatgcc gcgcctgtgg cagcggttct gcgaggcgct gggggcggac
      721 gatctgctgg cccatcccga ttatcagacc acggcggatc gcatcgcgcg caaggaccag
      781 ctcaaggagg cgatcaacgc catcacaagc cagcgcacgt cggccgatct ggtgggcgtc
      841 ctgaacgagg cgggcgtgcc gtgcggggcc atcaacacca ttggcgaagg tttcgaggat
      901 cctcaggtgc gccacctggg catggcgaag ccggcgccgc accctgatat gggttcggtg
      961 aacctcatcc gctcccccat caacctgtcg cgctttcctc aggagtcggc cttccgcaac
     1021 gcggctccgg accccggttt tgacacccgc gccgtgctga ccgagctcgg tattcccagc
     1081 tcccgcatcg acgcgctggc ggcggcgggc gccatcgcct gcaccgaccc ggcatcggcc
     1141 tgaagccgcc gtgaacgtcc aaccgctttc ggttcgggag cgcatgggct acggcgtggg
     1201 cgatttcggc gtcaatctgt tcttcatccc ggccatgacg tacctgctgt acttctacac
     1261 ggacgtgttc gggctgagcg cagcggccgc cggaggcgtg ttcctcgtgg cccgggtggt
     1321 ggacgcggtg accgacccgc tcatgggcgg gattgccgag cgcacgagca gccgctgggg
     1381 gcggcttcgg ccccacattc tcttcggcgc gctgcctctg ggggcgatcg ccgtgctcac
     1441 cttcaccacc ccggacctga acgatgccgg caagctctgg tgggcggccg tcacgtacgt
     1501 gctgttcggc atcgcctaca cggtggtctc cattccctac tcggcgctga ccgcgtcgat
     1561 cacggccgat catcacgagc ggacgctgct gaccaccgtg cgcatgggtt gcgcgttcgc
     1621 tggtggctac gtggtctccg tgggcatgct gcccctggtg ggtcttttcg agacccaagc
     1681 cgcgggtttt gcctgggcca tggtgatctt cgctacgttt gccacggggc tgctgtggct
     1741 gaccgtggcg aacacgcgcg agcgcgtgca gccgccggct acccagaagc tgacggttcg
     1801 tgacagcttc agggccgtgt tcgcaaaccc gccgctgctc gtcgtgatcg tgcttttcac
     1861 cggcggcatg ctctctttca ccatccgcca gtcggtcacc ctctactact tcaagtacaa
     1921 cctcggccgg gaggatctca tcgcgctttt cttcggcatc actcttcccg tgatgatggt
     1981 cggcctgctc ggggtgcccc ggctggcggc cgccgttggc aaagcgggcg gcatcgtcgt
     2041 gggggcgctt gtcactatcg tcggatgcgc gggtctgtac ctgacgccct acgactcggt
     2101 gacctgggtg ttcgtctggt cgtgcgtcat cgcccttggc gggacgccca tcgccgtgct
     2161 gggctgggcc atgatcccgg acacggtgga gtacgcccaa tggcgcatgg ggctcagagc
     2221 cgatggagcc atcttcggtt tcgcgagctt ctttcagaag ctggccaagg ccgtgggcgg
     2281 agcaggcgtc gcgggagctc tggccctggc cggctacgtg gccaacgtgg agcagtccga
     2341 ggcgtcgctc agagccattc acaacatgat gaccctggcg ccggcgctca tcatgctggt
     2401 gctgatcatc gccgcccggg cctaccgcct ggacgccgcg actcatcggc gaatcgtgca
     2461 ggagctctcc ggcgcgtgac gcgcccgggt aattctagtg aatgtccgcc gcgtcccccc
     2521 aggcgtcgcg caggctcgcc atgtcgaagc cgggctgtcg ggctgcctcg atgatgaccc
     2581 gctcgcggcg cgcgatggtg tccgccgccg cggcgacctc ggctgcgact tcccagggga
     2641 tcaccaccgc gccgtgctgg tcggcgtgaa tcaggtcgcc gccgttcacg cgcatgccgg
     2701 ccacgttcac cgtgcggccg aagtccaccg ggtgaacgta ggcgtgtgac gggccgatgc
     2761 ggtcggccag catctgaaaa ccttcggcga tgtccgggat gtcgcgcacg ctgccatcgg
     2821 tgatcacgcc gaggcagccg aaccccttgt gcacgttgct gttcacctcg ccccaccagg
     2881 agccgtagcc gcgctggtcg ccgtccatgt cctggatgac catgacgctg ggcttgggcc
     2941 cctcgtccac gtaggcgtag taggcgtcac gcagtgcctg tgcatcgctg ccgcgcaggt
     3001 gtccggggtg cgcggcacgg atggtggccg tgcgcgccac cgccaccagg ggcggcagct
     3061 ccgggcgggt gcagacaagg gggcgaacgg tgtagccgta gccgcgccgc tccggggcaa
     3121 ccacctccag cgcgttgcag acggtgggcg tatccagacg ctgaagctcg gcgatgaggt
     3181 ccgcggtgag ttcggtcatg ggctcttcct gacggttcca tacgctgaaa gtatcacgcc
     3241 cgcgcgcgga tgggccaagc gctgcgtcgg agctctttca ccggcgtgca cgttgcgaac
     3301 ggaatgctgt ttatcgtctt atcgtccggt taatcaacgt gcgcaagttc gccgactgag
     3361 cccggcctga cgaggtgccc gccgacgtgc cgggtgtcag gcgccgattc gcaccgacag
     3421 ctgatcgcac gttgcgccgt gcaggaccgc attggcggtc gaacccaccg caagactcgg
     3481 gcctgagcgt ccgtgcgtgc cgagcacgac gagatctgct ccgatgcgac tggccagccg
     3541 gtgaattgca cctgagaccg atccgggcac cgagtaccgc ctatcctcgg gaacctcgta
     3601 gagctcaccg aggctgtcga gcagctcgcg ttccatttgt tgggtatcga cgtcgtagcc
     3661 catgtacacg tggcacagag agagctcggc tccgctttgc tga
//
LOCUS       DBNHOX010000364         4512 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1571226, whole genome shotgun sequence.
ACCESSION   DBNHOX010000364 DBNHOX010000000
VERSION     DBNHOX010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1571226"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="AC16UZ_08030"
     CDS             complement(<1..679)
                     /locus_tag="AC16UZ_08030"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246668.1"
                     /translation="MSGGRLEGKVAIVTAAGRGIGRGIALRLAEAGADLVVNSYGEDT
                     TRSTAEAVEATGRKALQVPGDITDPEKMLELTERALETYGQIDVLVNNVGAGPKSAIE
                     PEPGPLGPVAALWDALYQQNLRPVVLMTEAVLPHLIARKSGRIIHISSIAGRTSLSAP
                     MLTHFVHNSYGAMKAGLINYTQTLAELVGPHNINVNAICPGIVWTDAWRANAERAVTA
                     LPEFAGQD"
     gene            869..1225
                     /locus_tag="AC16UZ_08035"
     CDS             869..1225
                     /locus_tag="AC16UZ_08035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6246669.1"
                     /translation="MTVIIAGIMELEDPSQVKEMLLAARPHIEGALQEEGCIEYAWTE
                     DHLNPGRVHVYEEWTSSETLEAHLQSHWYRDMGAHLRQYPRKPPTRVIKKYRVDLEEP
                     VYDSEGVARGHFFTES"
     gene            complement(1311..2192)
                     /locus_tag="AC16UZ_08040"
     CDS             complement(1311..2192)
                     /locus_tag="AC16UZ_08040"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6246670.1"
                     /translation="MVQLTTLPPTATTEEIMEVIHADGAVIVKDMLTGDEVGAVVDEV
                     RPYMDTTPVGADEFAGFKTTRTGALVARSPKCRDLIMNQTVLDATKAFLAPYCLRFQL
                     HLGQIIRLMPGQPAQQIHRDRWAWGKYLKDVEPQFNTIWALSDFTQANGATQVVPGSI
                     DWPDDRVAKPDEICQAVMSAGSALLYTGSVFHGGGENRSDGDRIGMNLTYSLGWLRQE
                     ENQYLSCPPEIARELPEEMQNLLGYQLGSYALGYYTPPLPPGEGPEAVGPQWAVGRRD
                     SHEALGDAELLEAVINM"
     gene            complement(2266..2988)
                     /locus_tag="AC16UZ_08045"
     CDS             complement(2266..2988)
                     /locus_tag="AC16UZ_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246671.1"
                     /translation="MKALKYVLLVVLVAVIGAVAYVVVNAGSIVKQVIETVGSDVLGV
                     PVTVRSVDLSLDGGSIGGLQISNPEGFETPYALRIEKAAVKINAQASTAKLIVLDQIL
                     VDQAEVNAEFVGRNGNLQTLQKNIEASVGPGEPETEASDVKVIIERFDFTNANTRLSS
                     DVLGGGQISVPDLHLTGIGRKESGVTAAEAAKQLFGPVIGAIVKAAVAQQTGIDQDKI
                     KGTISDKIGSGLKSLTGSKSDD"
     gene            3095..4207
                     /locus_tag="AC16UZ_08050"
     CDS             3095..4207
                     /locus_tag="AC16UZ_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6246672.1"
                     /translation="MANINRAALFFEPGKPFEIVELPIPEPAAGGLTLKVTRANICGS
                     ELHMWRGDGRFAQVITPNGRILGHEATGVVHALGEGVTTDWAGNALAEGDRIAYQYFQ
                     PCGRCRNCTRGMSEACRDSFSIQRGSLEEWPYTRGSFADYLYLHPRQAIFKVPDHVTD
                     TMVVSANCALAQVVMGLERVNVQLGDRVVVQGCGGLGVYACAIAKERGAELVIAIDGI
                     DERLELAQRMGADHVIDLRQVEDSKARVGAVKDLTGGLGADVVVEVVGFASVINEGLR
                     MLAWGGRYLEMGTFYTGTSFECDPGRLVGQNQRIEAVASYDAASLQRAIEFLARNADR
                     LPLDEVVVDYPLDQIERAFADQDSGLVRRASLVMGD"
     gene            4215..>4512
                     /locus_tag="AC16UZ_08055"
     CDS             4215..>4512
                     /locus_tag="AC16UZ_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR6246673.1"
                     /translation="MTEYDVIIIGAGMSGMYQLYRLRQLGLSVRVYEAGSGVGGTWYW
                     NRYPGARFDSESYSYGYSFSQELLDEWDWSEHFSAQPETLRYLNHVADKFDLRRD"
BASE COUNT          715 a         1431 c         1537 g          829 t
ORIGIN      
        1 gatcctggcc ggcgaactcg ggcagcgccg tgacggcccg ctccgcgttg gcccgccagg
       61 cgtccgtcca gacgatgccg ggacagatgg cgttgacgtt gatgttgtgt ggaccgacga
      121 gctcggccag ggtctgggtg tagttgatga gcccggcctt catggcgccg taggagttgt
      181 gcacgaagtg ggtcagcatc ggcgccgaca gcgaggtgcg cccggcgatg gacgagatgt
      241 gaatgatcct gccgctcttc cgggcgatga ggtgaggtag aaccgcctcg gtcatcagca
      301 ccacgggccg aagattctgc tgatagaggg cgtcccacag cgcggccacc gggcccaggg
      361 ggccgggctc gggctcgatg gccgacttgg gcccggcgcc cacgttgttg accagcacgt
      421 cgatttgacc gtaggtctcc agggcccgct cggtgagctc gagcatcttt tccggatcgg
      481 tgatgtcccc cgggacctgc aacgccttgc ggccggtggc ctccacggcc tcggccgttg
      541 atcgcgtggt gtcctcaccg tatgagttca ccaccagatc ggcgccggcc tcggccaggc
      601 gcagggcgat gcctcgcccg attcccctgc cggcggcggt gacgatggca actttgcctt
      661 ccagcctgcc accgctcatc gcgcggtccc cccgtcccgt cccggcgtct ggcggctcga
      721 ctctatgatt gcccgctgtc tgcgttgacc gttcagctcc tctcccatca gcctttgctc
      781 cctggtggcg gttccctcgc atgccgaggt gattgagata gactttgcat ggcaatttcg
      841 ggatcagtca actgcgggag aacgatcgat gacggtgatc attgcgggga tcatggagct
      901 cgaggatccg agccaggtga aggagatgct gctggccgcg cgcccgcaca ttgaaggcgc
      961 gctgcaggag gagggctgca tcgagtacgc gtggaccgag gaccatctga accccgggcg
     1021 cgtgcacgtc tacgaggagt ggacgtcctc tgagacgctg gaggcccatc tgcagagtca
     1081 ctggtatcgt gatatggggg ctcatctgcg ccagtatccg cgcaagccgc ccacccgggt
     1141 catcaagaaa taccgcgtcg acctggaaga gcccgtctac gattccgaag gcgtcgcgcg
     1201 cgggcacttc ttcaccgaaa gctaggatcg ggaattgatc tggttccaga cctgatcggg
     1261 agggcgtgcg cccgtttttc ccagcgcacc gccgtcactg gcggccgacg ctacatgttg
     1321 atgaccgctt ccagcagctc cgcgtcgccc agggcctcgt ggctgtcgcg ccgccccacg
     1381 gcccactgcg ggccaacggc ctcgggccct tcaccgggag gcagcggcgg ggtgtagtac
     1441 cccagggcgt agctgccgag ctggtagccc agcaggttct gcatctcctc gggcagttcc
     1501 cgggcgatct cgggtgggca ggagagatac tgattctcct cctgtcgcag ccaccccagg
     1561 gagtaggtca ggttcatgcc gatgcgatcc ccgtcggagc ggttctcgcc gccgccgtgg
     1621 aagaccgatc ccgtgtagag cagggccgat ccggcgctca tgacggcctg acagatctca
     1681 tcgggtttcg ccacccgatc gtccggccag tcgatgctgc ccggcaccac ctgggttgcg
     1741 ccgttggctt gggtgaagtc ggacagcgcc cagatggtgt tgaactgggg ctccacgtcc
     1801 ttgaggtact tgccccaggc ccagcggtca cggtgaatct gctgggccgg ctgcccgggc
     1861 atgaggcgga tgatctgccc caggtgaagc tggaaccgca ggcagtaagg tgccaggaag
     1921 gccttggtgg cgtccagcac ggtctggttc atgatcagat cccggcattt cggggatcgg
     1981 gcgaccagcg cgccagtgcg cgtagtcttg aagccggcaa actcgtccgc tccgaccggg
     2041 gtcgtgtcca tgtacggccg cacctcgtcg accaccgcgc cgacctcgtc gcccgtgagc
     2101 atgtccttga cgatcaccgc gccgtcggcg tgaatgacct ccatgatctc ctcggtggtc
     2161 gccgtggggg gcagggtggt gagttgaacc atgacgtcgc gcctctctct gcttcagcgg
     2221 gatgattgaa tcccaagacc gtccggccgg caagccgtgc cgggctcagt cgtcggactt
     2281 gctccccgtc aggcttttca ggccagagcc gatcttgtcc gagatggtgc ccttgatctt
     2341 gtcctgatcg atgcccgtct gctgagcgac ggctgccttc acgatggcgc cgatcaccgg
     2401 gccgaacagc tgcttggcgg cctccgccgc ggtgacgccg ctctccttgc ggccgatccc
     2461 ggtcaggtgc aggtcgggga cggagatctg cccgcctccc agcacgtcgg aggaaaggcg
     2521 cgtgttggca tttgtgaagt caaagcgttc gatgatcacc ttgacgtcgg atgcttccgt
     2581 ctccggctcg ccggggccca cggaggcttc gatgttcttc tgcagggtct gcaggttgcc
     2641 gttcctgccg acgaactcgg cgttcacctc ggcctggtcc acgagaatct gatccaggac
     2701 gatgagcttg gcggtgctgg cctgggcgtt gatcttcacc gccgccttct cgatcctgag
     2761 ggcgtaaggg gtttcgaacc cctcggggtt ggagatctgc aagccgccga tgctgccgcc
     2821 gtccagggag aggtcgacgg accggacggt gaccggaacg ccgagcacgt ccgaccccac
     2881 cgtctcgatg acctgcttga cgatgcttcc cgcgttgacc accacgtagg cgacggcgcc
     2941 gatcactgca accagcacga ccagcaggac gtacttcaat gccttcatcg atcctcccct
     3001 ttgctctagg atgcgccaag tggggcgcgt ttctgggggc acgttgccct gaaacgctgg
     3061 cggattcaag gtcttcggac ggggggaggt acacatggca aacatcaaca gagcggcgct
     3121 gtttttcgag ccgggcaaac cgttcgagat cgtcgagctg ccgattcccg agccggcggc
     3181 cgggggactg acgctcaagg taacccgggc caacatctgc ggatccgagc tgcacatgtg
     3241 gcggggtgac ggccgcttcg cccaggtcat cacgcccaac ggacgcattc tgggccacga
     3301 agccaccggt gtggttcacg cccttggcga gggggtcacg accgactggg cgggcaacgc
     3361 gctcgcagaa ggggaccgga tcgcctatca gtactttcag ccctgcggtc gctgccgcaa
     3421 ctgcacccgg ggcatgagcg aggcctgccg ggacagcttc agcatccagc gcggcagcct
     3481 ggaggagtgg ccttacaccc gcgggtcgtt tgccgactac ctgtacctgc atccgcgcca
     3541 ggccatcttc aaggtgccgg accacgtcac cgacaccatg gtggtgagtg cgaactgcgc
     3601 gctggcccag gtggtcatgg gtctggaacg tgtcaacgtg cagctgggcg accgggtggt
     3661 ggttcagggc tgcggggggc tgggcgttta tgcctgcgcg atcgccaagg agcggggcgc
     3721 ggagctcgtg atcgccatcg acggcatcga cgagcgtctg gagctcgctc agcgcatggg
     3781 cgcggatcac gtcatcgacc tgcgtcaggt ggaggactcc aaagccaggg tgggcgccgt
     3841 caaggacctc accggcgggc tcggcgcgga cgtggtcgtc gaggtggtgg ggtttgccag
     3901 cgtgatcaac gaaggcttga ggatgctcgc ctggggcggc cgttacctgg aaatgggcac
     3961 gttctacaca ggcacgagct tcgagtgcga cccgggcagg ctcgtcgggc agaaccagcg
     4021 catcgaggcg gtggcctctt acgatgcggc cagcctgcag cgcgccatcg agttcctcgc
     4081 ccgcaacgcg gaccgcctgc ccctggacga ggtggtggtg gactacccgc tggatcagat
     4141 cgagcgggca tttgcggacc aggactcagg actcgtgcgg cgtgcctccc tcgtgatggg
     4201 ggattgatgc gacgatgacc gagtacgacg tcatcatcat cggcgccggc atgtccggga
     4261 tgtaccagct ctaccggctg cggcagctgg gtctgtcggt gcgcgtctac gaggcgggca
     4321 gcggcgtggg cggcacgtgg tactggaacc gctatccggg tgcgcggttc gactcggaga
     4381 gctacagcta cgggtactcg ttctcccagg agctcctgga cgagtgggac tggagcgagc
     4441 acttctcggc ccagcccgag accctgcgct acctgaatca cgttgcggac aagttcgacc
     4501 tgcggcgtga ca
//
LOCUS       DBNHOX010000365         3155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_286323, whole genome shotgun sequence.
ACCESSION   DBNHOX010000365 DBNHOX010000000
VERSION     DBNHOX010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_286323"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="AC16UZ_08060"
     CDS             <1..207
                     /locus_tag="AC16UZ_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246674.1"
                     /translation="LIAATQEEIGRAALMEQLNQSFGLITPEMTEVVDGVKGILFGEA
                     STDSPIAAPPYAIGESEFVSPLAM"
     gene            232..768
                     /locus_tag="AC16UZ_08065"
     CDS             232..768
                     /locus_tag="AC16UZ_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246675.1"
                     /translation="MFRRIFVPAFFISLVAGCTSTETVDACAVRFQDDLERSMSLAED
                     RLENGCEYRFDGYFQSLLAIAAENPDSRNPMLFSDHLMRVNDMGVISKRQAELLYNRY
                     FNVKFVSLRGDYNTCSQTCPMRDQVMSDMRAELHDKELGLMRASADRASFYRADTLLK
                     EADLVLEATCRACGADGR"
     gene            769..1377
                     /locus_tag="AC16UZ_08070"
     CDS             769..1377
                     /locus_tag="AC16UZ_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246676.1"
                     /translation="MKGVAEFVCGAGAGAGLALVALGLSGGAVAADAAPVSDWLQLNL
                     GHSAWLFALVLTVYAANLHRLSRLLAGGPDLKVVVKLDQLSDVWINLFVGIGVIWTAV
                     GMRSALQAALGDPGGALSGSAGTVLHKLVDGGILLALTTTIVGGVGGYVMRLVKTMVV
                     GARLQTFYSGVSRADLRELVGLGERIEARLARLDPQEPAVVA"
     gene            1374..2084
                     /locus_tag="AC16UZ_08075"
     CDS             1374..2084
                     /locus_tag="AC16UZ_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246677.1"
                     /translation="MTFEPVNHAADGDGLQTDVMRFMAIIAFCLIAVLALVKNAEPAV
                     DPQPAPSVQQPVGMAQASELAAPEVEPRAAVRPARPQPVPPVATGTPQERPAEVEDVE
                     DVEDVEKVPGEPAAPHGLALRFASDRDFLRLIETGRVRVFAFQPDDPGGTALGLGRNL
                     EFFASPPPGRVYELQADPIPSRVTASLARAAGNPGGFSWGVRLPVELEASIRSWIDQD
                     VIGELIIDRYAQVRHVGS"
     gene            2071..>3155
                     /locus_tag="AC16UZ_08080"
     CDS             2071..>3155
                     /locus_tag="AC16UZ_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246678.1"
                     /translation="MSAARCLATWLLLATLPGLASAAPGGELLGGSLGRWLDETAGPE
                     LAETLARHPKFKGETVRFVPMLDGRAEPAGNRLVQAVEQRLTRHLIREGGVRIAWERD
                     RQPCAASEEVPYLLGIEIQQRGPDIYHLNIAMVDVDEGVWVSGVSLAWKGRLLPTERT
                     ALGTRANGGPPGSMQSPLPLSDSSVIGELMLAGLGCAIPAGLDGSLYVAMPVESDLSG
                     VAQALTRQLSVRPLAALTIDRAQASWEMALTARPLGNGTDELVATLRDLRREGSAQQI
                     ASGVISRPRSERPGPVTPLGMPAALLSGLSYETARSRGVCQSSTGPGGCIEVSFDLLR
                     PAYLLVVSSRDERLESQVCGRSLRIAEP"
BASE COUNT          473 a         1045 c         1090 g          547 t
ORIGIN      
        1 ctcatcgccg cgacccagga ggaaatcgga cgcgcggcgc tgatggagca gctcaaccag
       61 tcgttcgggc tgatcacgcc ggagatgacc gaagtggtcg acggcgtgaa gggcatcctg
      121 ttcggcgagg cttccaccga cagccccatt gcggcgcccc cctacgccat cggcgagtcc
      181 gagttcgtca gcccgctggc catgtagcgc cgattcgatc aggagaaaga catgtttcgt
      241 aggatatttg ttccggcctt tttcatctcg ctcgtcgctg gctgcaccag cacggagacc
      301 gtggatgcct gtgcggtgcg atttcaggac gacctcgagc gcagcatgag cctcgccgag
      361 gatcggctgg agaacggatg cgagtaccgc ttcgatggct actttcagtc cctgctggcg
      421 atcgccgcgg aaaatccgga ctcgaggaat cccatgctct tcagcgatca cctgatgcgc
      481 gtgaacgaca tgggcgtcat cagcaagcgc caggcggagt tgctctacaa ccgttacttc
      541 aacgtcaagt tcgtctccct gcgcggcgac tacaacacgt gctcgcagac ctgccccatg
      601 cgcgatcagg tcatgtccga catgcgcgcg gagctgcacg acaaggagct tggcctgatg
      661 cgggccagcg cggaccgggc gagcttctac cgggcggaca ccctgctcaa ggaagccgat
      721 ctcgtcctgg aagcgacctg tcgggcttgc ggcgcggacg ggcgctgaat gaaaggcgtc
      781 gcggagttcg tgtgcggagc gggcgcgggc gcggggctcg cgctggtggc cctgggcctc
      841 tcgggcgggg ctgttgccgc ggacgctgca cccgtgagcg actggctgca gctcaacctg
      901 gggcactccg cctggctctt cgctctggtg ctgacggtct atgccgccaa cctgcaccgg
      961 ctgagccgac tgctcgccgg cggcccggac ctgaaggtgg tcgtcaagct cgatcagctc
     1021 tccgacgtct ggatcaacct gttcgtcggt atcggggtca tatggacggc cgtcggcatg
     1081 cgcagcgcgc tgcaggcggc ccttggtgat cccggcggcg cgctgtcggg ctccgcaggc
     1141 acggtccttc acaagctcgt cgacggaggt attctgctgg cgttgacgac gaccatcgtc
     1201 ggcggggtcg gaggctacgt catgcgtctg gtcaagacga tggtggtcgg cgcccgcctg
     1261 cagaccttct actccggcgt tagccgtgca gaccttcgcg agctggtggg ccttggcgag
     1321 cggatcgagg cccgtctggc ccgtctcgac ccccaggaac cggcggtggt ggcatgacgt
     1381 tcgagccggt caatcacgcc gccgacggcg atggcctgca gactgacgtc atgcggttca
     1441 tggcgatcat tgccttttgc ctcatagccg tgctcgccct tgtcaaaaac gccgagccgg
     1501 cggttgatcc gcagcccgcg ccgtcggtgc agcaacccgt cgggatggcg caggcgtcgg
     1561 aacttgcggc cccggaggtc gaacctcgcg ctgccgtgcg ccccgcgcgc ccgcaaccgg
     1621 tgccgccggt ggcgacggga acgccccagg agaggccggc agaagttgag gacgttgagg
     1681 acgttgagga cgttgagaag gtaccgggcg aacctgccgc tccccatggg ctcgcgttgc
     1741 gctttgcctc ggatcgggat ttcctgcgcc tcatcgagac gggacgcgtc cgtgtattcg
     1801 cctttcagcc ggacgatccg ggcggcaccg cgctcggcct cggccggaac ctggagttct
     1861 tcgcctcccc gcccccaggc cgcgtctacg agctccaggc agaccccata ccgtcgcgcg
     1921 tgaccgcttc cctcgcccgg gcagccggga acccgggcgg cttttcctgg ggcgtgcgcc
     1981 tgccggtcga gctggaagcg tcgatccgca gctggatcga ccaggacgtg attggggagc
     2041 tgatcatcga ccgatatgcg caggtgcgac atgtcggcag ctaggtgcct ggcgacgtgg
     2101 ctactgctgg cgactctgcc gggtctggcg tccgcggcgc ctggcggcga gctgctcggc
     2161 ggatccctgg gcagatggct cgacgaaacg gcgggtccgg agctggccga gaccctcgcc
     2221 cgccacccca agttcaaggg agagaccgta cgcttcgtgc ccatgctgga cggtcgcgcg
     2281 gaaccggccg gcaaccggct cgttcaggcg gtcgagcagc ggttgacccg tcacctcatc
     2341 cgcgaaggag gggtgcgaat cgcctgggag cgcgaccggc agccctgtgc tgcgtctgag
     2401 gaggttccct atctgctggg catagagatt cagcagcggg gcccggacat ctatcacctc
     2461 aacatcgcca tggtcgacgt ggacgaaggc gtctgggtca gcggcgtcag cctcgcctgg
     2521 aagggccgcc tgctcccaac ggagcggacg gcgctcggta cccgagcgaa cggcggtccg
     2581 cccgggtcga tgcagagccc gctgcctttg agcgacagca gcgtcatcgg cgagctcatg
     2641 ctggcgggcc tgggctgcgc gattcctgcc gggctcgacg gctcgctcta cgtggccatg
     2701 cccgtggagt ccgatctgtc cggcgtcgct caggcactca cccggcagct cagcgtccgg
     2761 cctctggccg cgttgaccat tgatcgcgcc caggcgagct gggagatggc gctgacggcg
     2821 agaccgctgg gcaacggaac agacgagctc gtcgcgaccc ttcgtgacct gcgccgggag
     2881 ggctccgctc agcagatcgc ttccggggtg atatccaggc cgcgcagcga gcgcccgggc
     2941 ccggtgacgc cgctgggcat gccggctgcg ctgctttccg gattgagcta cgaaaccgcg
     3001 cgcagccggg gcgtctgcca gtcgtcgacc ggacccggcg gctgcatcga ggtgtctttc
     3061 gatctgctgc gtccggccta cctgctggtc gtctccagtc gcgatgagcg tctggaatct
     3121 caggtctgcg gacgcagcct ccgtatcgcc gagcc
//
LOCUS       DBNHOX010000366         3042 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1057827, whole genome shotgun sequence.
ACCESSION   DBNHOX010000366 DBNHOX010000000
VERSION     DBNHOX010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1057827"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /locus_tag="AC16UZ_08085"
     CDS             complement(<1..1784)
                     /locus_tag="AC16UZ_08085"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HIR6246679.1"
                     /translation="MNTSASDAAARSLAVIEETRQHGSIGHLPVWENITAAHVEAGVR
                     ALLDESEEAFTALETDVEASWAGLMEPLERLQTRIGQVIGAVTHLISVKYSDELQAAY
                     DAIRPIYVSLLNRMSQSLGVYGGLVALRDGPDWPALSQARQRIVTESIRAMERSGVHL
                     EGPPKERFKEIQERLSELGNDFSVNLVKEEKASRVRVQAPEEVAGVPAAVLGLAAAKA
                     REDGEDGATDASGPWHFVVNGVNFVAVMEHALDRGLRERFYRAFRARGTSRDFDNRCV
                     LTEILRLRQEQSALVGFECYADRSLDAKMAPDTDAVWQLFNELEAAARPAAEREFETL
                     KAYMRDEGGLDEGEQPAPWDLAFWSEKMRESLYAYDGERLREYFQLPRVLDGLFSLVG
                     RLYEVEVRRVAAGAVPVWDPAVEFFEVIGAGEVIAGFYVDPYARPGEKRGGAWMNTVV
                     SRSRVLAAPGKSSSLPVALFVMNARPPAEGKVALLSLDEVRTLFHEFGHATQHMFTTI
                     EDGGASGMNLVEWDAVELASQFNEYWMEHKPFLRDLTAHFDTGEPLDDETLDRIVDSR
                     NFMVGTATLRQLFFGKVDMTLHQRFGVAG"
     gene            complement(1912..2790)
                     /locus_tag="AC16UZ_08090"
     CDS             complement(1912..2790)
                     /locus_tag="AC16UZ_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR6246680.1"
                     /translation="MARENEDRNLALFCDFENVALGVRDAKYARFDIQLVLERLLVKG
                     SIVMKKAYCDWDRYKEFKPVMHEAAFELIEIPHTRQSGKNSADIRMVVDALDLCYTKE
                     HIDTFVIISGDSDFSPLVSKLRENAKTVIGIGVKNSTSDLLIGNCDEFIFYDDLVRHQ
                     KATRKPRKKPAAKKAPEKKDATDATDAMDGKDAKEAKRAEPARSEEGNTDEGIALVLE
                     TAEALYGERGEREKLWGSMVKQTLKRRRPGFNESFYGFRSFSDLLEEAESRGLLELEM
                     DDRSGGYLIRRLTREE"
BASE COUNT          511 a         1020 c          952 g          559 t
ORIGIN      
        1 cccgcgacgc cgaatctctg gtgcagggtc atatcgacct tgccgaaaaa gagctgccgc
       61 agggtggccg tccccaccat gaagttgcgg ctgtcgacga tccgatcgag cgtctcgtcg
      121 tccagcggct ccccggtatc gaagtgggcg gtgagatcgc gaagaaaggg tttgtgctcc
      181 atccagtact cgttgaactg gctggcgagc tccacggcgt cccactcgac gaggttcatg
      241 cccgatgcgc cgccatcctc gatggtcgtg aacatgtgct gggtggcgtg gccgaactcg
      301 tgaaagagcg tgcgcacctc gtccagcgac agcaacgcca ccttgccctc tgccgggggg
      361 cgcgcgttca tgacaaacag ggccacgggc agcgacgacg acttaccagg cgcggcgagc
      421 acccggctgc gcgacaccac ggtgttcatc cacgcgccgc cgcgcttctc gccaggccgg
      481 gcgtagggat ccacgtaaaa gccagcgatg acctcgccgg ccccgatcac ctcgaagaac
      541 tcgaccgcgg gatcccagac gggcaccgca ccggccgcca cgcgacgcac ctcgacctcg
      601 tatagccgac caaccaggga aaacaggccg tcgagaacgc ggggaagctg aaaatactcg
      661 cgcaggcgct caccgtcgta agcgtagagc gactcgcgca tcttctcgga ccagaatgcc
      721 agatcccagg gcgcgggctg ctccccttcg tcgagaccgc cctcatcgcg catgtacgcc
      781 ttgagcgttt cgaactcgcg ctccgcagcg ggccgcgcgg ctgcctccag ctcgttgaaa
      841 agctgccaga cggcgtccgt gtccggtgcc atcttggcgt ccagactgcg gtcggcatag
      901 cactcgaacc ccacgagggc ggactgctcc tggcgaagcc ggaggatttc cgtgagcacg
      961 caccggttgt cgaagtccct ggatgtgcca cgggcgcgaa acgcccggta gaaccgctca
     1021 cgcagccccc gatccagcgc gtgctccatg acggcgacga agttcacgcc gttgacgacg
     1081 aagtgccacg gaccggaggc gtccgtagcg ccgtcctccc cgtcttcccg ggccttggcg
     1141 gctgccagcc cgaggacggc cgcgggcact ccggcaacct cttcaggcgc ctgcactcgg
     1201 acccgcgacg ctttctcctc tttgaccagg ttcacggaga aatcgttgcc gagctcggac
     1261 aggcgctcct ggatctcctt gaagcgctcc ttggggggcc cttccagatg cacgcccgaa
     1321 cgttccatgg ccctgatgga ctccgtgacg attcgctgcc gagcctggct cagtgccggc
     1381 cagtcggggc cgtcccgcaa cgcgaccaga cccccgtata cgcccaggct ctggctcatc
     1441 cgattgagca gcgacacgta gatggggcga atcgcgtcgt aggccgcctg gagctcgtcg
     1501 gagtacttga cgctgatcag gtgggtcacc gcgccgatca cctgaccgat gcgcgtctgc
     1561 aggcgttcca ggggctccat gagcccggcc caggacgcct cgacgtcggt ttccaatgcg
     1621 gtgaaagctt cttcgctctc atcgagcagg gcgcgaacgc cggcctccac atgagccgcc
     1681 gtgatgttct cccatacggg caggtgcccg atggatccgt gctgccgggt ttcctcgatg
     1741 acggccaggc tgcgggcagc cgcgtcagat gccgatgtgt tcacgatgat ctccttggca
     1801 agcctctcgg ggccggcgtg ctttgagcag cgaggtttgt ccacacgatg aggcggtttc
     1861 tcccctcgtc cgtccggcgg gtcccggcga ggggaagcgc attctaaggc gtcattcttc
     1921 ccgggtcagc cggcggatca ggtatccgcc ggagcggtcg tccatttcca gttccagcag
     1981 gccccggctt tccgcttcct cgaggagatc gctgaacgag cggaagccgt agaaactctc
     2041 gttgaacccc gggcgccgcc gcttgagcgt ctgcttcacc atggagcccc agagtttttc
     2101 ccgctcgcca cgctcgccgt acagcgcttc agcggtctcc agcaccagcg cgatcccctc
     2161 atcggtgttg ccctcttccg agcgggcggg ctcggccctc ttcgcctctt tggcgtcctt
     2221 gccgtccatg gcgtccgtgg cgtccgtggc gtcctttttc tcaggcgcct tctttgcggc
     2281 cggcttcttg cgtggcttgc gggtcgcctt ctgatggcga accagatcgt cgtaaaagat
     2341 gaactcgtcg cagttgccga tcaggaggtc cgacgtcgag ttcttcacgc cgatcccgat
     2401 gacggtcttc gcattctccc tgagcttgct gaccagcggc gagaaatcgg agtcgccgct
     2461 gatgatcacg aacgtatcga tgtgttcctt ggtgtagcag agatccaggg cgtccacgac
     2521 cattctgatg tccgccgagt ttttaccgga ctggcgcgtg tggggaatct cgatcagctc
     2581 gaacgccgcc tcgtgcatga cgggcttgaa ctccttgtag cgatcccagt cgcaataggc
     2641 tttcttcatc acgatgctgc ccttgaccag caggcgctcg agcaccagct ggatgtcgaa
     2701 cctggcgtac ttcgcatcac gcacgccgag ggcgacgttc tcgaagtcac agaagagcgc
     2761 aaggtttcgg tcttcgtttt cgcgggccat gaggtatgcc gcagctgagg ttgcgcctac
     2821 gttaacgggg ccgcgtgcgg ccgtccacaa gcaaacggtc acgcaaggcg gcgaccaggg
     2881 gatcgcaggg ctcagatcga cgcaagcgga agtgacagcg cctccccgac ccgacggggc
     2941 cgcccccact gggcgtgatc gggttcacga tttcggggca caagcgtgcg gatcggcgag
     3001 ggcgccggtg cctgctgaga cacaggtaac tgaatggccc aa
//
LOCUS       DBNHOX010000367         1603 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_158311, whole genome shotgun sequence.
ACCESSION   DBNHOX010000367 DBNHOX010000000
VERSION     DBNHOX010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_158311"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="AC16UZ_08095"
     CDS             <1..938
                     /locus_tag="AC16UZ_08095"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR6246681.1"
                     /translation="LVMLDARSGEILALVNQPSYNPNELGSVEVGNMRNRAVTDVYEP
                     GSTVKPFTVLAALESGRFAADTVIDTGPGYFRVGGKLVQDPVNRGAITVGEALQVSSQ
                     VGIAKIALDLEERAVYDVLSRAGVGEPVNTGLPGEAWASLDDAGLSSLVGRATLAYGY
                     GLAISPLQLAQAYLTLATGGVRVPVSIVKQEVVRPGTRVFDADMIADVVGMMEGVTDN
                     DGTAPKARVVGYRVAGKTGTARKVGASGYDDERHVALFAGIAPVSDPRLVIVVVVNEP
                     KGDARGGGSVAAPVFGRVAARSMRLLGVRPDGESA"
     gene            935..>1603
                     /locus_tag="AC16UZ_08100"
     CDS             935..>1603
                     /locus_tag="AC16UZ_08100"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF019850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR6246682.1"
                     /translation="MSVSLRQLLEDPDAPEVPIRGLAVDSREVRPGDAFVAISGDRSD
                     GQDYVAEACRNGAVAVLSERPLAAGLELEGCPAVVVPDLKLRRGALAARVHEWPSRRL
                     RCIGVTGTNGKTSIAHFIADLAGRLGRTAGYMGTIGWGRPGALERTRLTTEDGVTVQR
                     RLGQLLDAGCDWVAMEVSSHALSQGRVAEVEFDVAVLSNLSRDHLDYHQTEAAYADAK
                     ASLFR"
BASE COUNT          232 a          519 c          588 g          264 t
ORIGIN      
        1 ccctcgtcat gctcgatgcg cgcagcggcg agattctcgc cctggtcaat cagccgtcct
       61 acaaccccaa cgagctcggc tcggtggaag tggggaacat gcgcaaccgc gcggtcacgg
      121 acgtgtacga gcccgggtcc accgtaaagc cgttcacggt gctcgccgcc ctcgagtccg
      181 gtcgcttcgc cgccgatacg gtcatcgata ccgggccggg ttacttccgg gttggcggca
      241 agctggttca ggacccggtc aaccggggtg ccatcaccgt cggcgaagcg ctgcaggtat
      301 ccagccaggt gggtatcgcg aagatcgcgc tggatctgga ggagcgggcc gtgtacgacg
      361 ttctcagtcg tgccggtgtg ggtgagcccg tcaataccgg cctgccgggc gaggcctggg
      421 cgtcactcga cgacgcgggc ctctccagcc tggtgggccg tgccacgctg gcctacggtt
      481 acggtctcgc catctcgccc ctgcagctcg cacaagccta cctcacgctt gcaaccggtg
      541 gcgtccgggt gccggtgtcc atcgtgaagc aggaagtcgt gcgtccgggc acgcgcgtgt
      601 tcgatgcgga catgattgcg gacgtcgtcg ggatgatgga gggcgtcacc gacaacgacg
      661 gcaccgcgcc caaggcccgc gtggtgggct accgggtggc aggcaagacg ggcactgccc
      721 gcaaggtggg cgcctcgggc tacgacgacg agcgccacgt cgcgctgttt gccggaatag
      781 cgccggtatc ggatccgcga ctggtcatcg tcgtggtggt caacgaaccc aagggcgatg
      841 cccgcggcgg cggcagcgtt gccgcgccgg tgtttggtcg ggtggccgcg cgctccatgc
      901 gtcttctggg cgtacgcccg gacggggagt cagcgtgagc gtatccctgc ggcagctgct
      961 cgaagacccc gacgcccccg aggtgccgat cagggggctg gcggtggact cccgggaggt
     1021 tcgtccgggg gatgcgttcg tggcgatctc cggggatcgt tcggacgggc aggattacgt
     1081 cgccgaagcc tgccgtaacg gcgcggtggc cgtgctgtcg gagcgaccgc tggcggcggg
     1141 gctggagctg gaaggctgcc cggccgtggt cgtgcccgat ctcaagctgc gccggggcgc
     1201 cctggcagct cgcgtgcacg agtggccgag ccgccggctg aggtgtatcg gggtgacggg
     1261 gacaaacggc aagacgtcca ttgcccactt catcgccgat ctggccggac gcctcggccg
     1321 gaccgccggc tacatgggca ccatcggctg gggcaggccc ggggcgttgg aacgcacgag
     1381 gctcaccacc gaggatgggg tgaccgttca gcgccgtctg ggccagcttc tggatgcggg
     1441 ctgcgattgg gttgccatgg aagtcagctc ccacgccctc agtcaggggc gggttgccga
     1501 ggtggagttc gacgtcgccg tgctgtccaa tctgtcccgc gaccacctcg actaccacca
     1561 gaccgaggcg gcctacgcgg atgccaaggc atccctgttc cgg
//
LOCUS       DBNHOX010000368         3040 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1122614, whole genome shotgun sequence.
ACCESSION   DBNHOX010000368 DBNHOX010000000
VERSION     DBNHOX010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1122614"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..767
                     /locus_tag="AC16UZ_08105"
     CDS             195..767
                     /locus_tag="AC16UZ_08105"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007998187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR6246683.1"
                     /translation="MVLASGSPRRAELLRQIGLPFVVRPPDVDESLRPGESPERFVER
                     LAIGKARAVVAEDGPALGADTAVVVDEEILGKPADRASAIDMILRLSGRTHRVLTGVA
                     VTDGRAVRSCVVAADVTFRAITRDEAAAYWETGEPRDKAGGYGIQGIGGIFAQSINGS
                     YSAVVGLPLAETEALLHAIGVNTWELRASV"
     gene            760..2253
                     /locus_tag="AC16UZ_08110"
     CDS             760..2253
                     /locus_tag="AC16UZ_08110"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR6246684.1"
                     /translation="MSEELLINVNDFESRVALVSGSVVEEVQVARAGADSLTGNIYLG
                     RVQRIIPGMQAAFVDCGLERPGFLHVSDIAGPRLMLGEEPDGAVDIRELLHDGQQLMV
                     QVAKDPIASKGARLTTQLTIASRYLVLMPFSDHIGISQRVDEEDERERLRLLVDEVRG
                     GQDAGVGFIVRTAADGVDREPLEADVRVLLHIWDRIMARRAECGCPGIVYEELPLHTR
                     VIRDMATAELECIRIDHPPTLERVRKFVAEFLPELTDKVVLYDEPRPIFERFGVDDEL
                     TRALGSRVELKSGGHLVIEQTEAMTTVDVNTGAYLGKHNLEETVFRTNLEAAQVLPRQ
                     LRLRNLGGIIVVDFIDMEDEEHRRQVLRALEKACEGDPARIRIEGFSSLGLVQMSRKR
                     TRESLVQQLCEPCSHCHGAGVVKTAQSTCIEIFRSIMQDAESGCGAHSQGDYVIRAAD
                     NVVDRLLDEDADRLAAMAARVGRRVRIELEPSYGAGEFDIVLVGDTQ"
     gene            2392..>3040
                     /locus_tag="AC16UZ_08115"
     CDS             2392..>3040
                     /locus_tag="AC16UZ_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246685.1"
                     /translation="MHRIVLRPFLVVVTLTMLFVALMQGTGRLGFMFLPDLELVANQW
                     LSGQRVRVSGLEGSWRYLNPVVRAARVDLPAGHLREVVVELDWLESLVRNRFVARRVL
                     VGDGAVRLEKTPAGKWRLAGTSGDGEFDASGIVYHADELRFSGVIAFGLAGEEEAQSV
                     QVDYLATNRGGLHRHRLHLDNQDADCAADCGLHLEYQARDGRWPFWDERIQLSLDG"
BASE COUNT          498 a          926 c         1083 g          533 t
ORIGIN      
        1 ctttccgccg atcgcggctg gagctgggcc gtgctggtcc ccgcgctcac gagcatgctg
       61 gtctggccgt tcgtctccct gtttctgcag cgcctgcggc tgcaattcgg cgtcgagtga
      121 gtccggcgtc ggtgccagcc ggccgttcgg cgagccattc ggcgagccat tcggcaagcc
      181 attcggcgag ccacatcgtg ctggcttccg ggtcgccgcg ccgcgccgag ctgcttcgtc
      241 agatcggcct tcccttcgtc gtccgcccgc cggacgtgga cgagtcgctt cgcccgggtg
      301 agtcccccga gcgattcgtg gagcgcctcg cgatcggcaa ggcgcgggcg gtggtggcag
      361 aggacgggcc ggccctcggg gccgataccg ccgtggtcgt ggatgaggag atcctcggca
      421 agccggcgga tcgggcatcg gccatcgaca tgatcctgcg cctctccgga cgcacccatc
      481 gggtgctcac gggggtggcg gtgaccgacg ggcgggccgt gcggtcctgc gtggtggccg
      541 ctgacgtgac gtttcgggcc atcacccgcg atgaggctgc cgcgtactgg gagacgggcg
      601 agccgcgcga caaggcgggc ggttacggta ttcaggggat cgggggtata tttgcgcaaa
      661 gcatcaacgg cagctacagc gcggtggtcg gcttgcccct ggcggagacc gaagcgctgc
      721 ttcacgccat tggcgtgaac acgtgggagc tgcgggcaag tgtctgaaga actgctcatc
      781 aacgtcaacg acttcgagtc ccgggttgcg ctggtgtcgg ggtccgtcgt cgaggaggtg
      841 caggtcgccc gggccggcgc agacagcctg accggcaaca tctatctggg tcgcgtccag
      901 cgcatcattc cggggatgca ggccgcgttc gtcgactgtg gcctggaacg ccccggcttc
      961 ctgcacgtca gcgacatcgc aggcccccgg ctgatgctcg gcgaagagcc ggacggggcg
     1021 gtcgacattc gcgagctgct gcacgacggc cagcagctga tggtccaggt ggcgaaagat
     1081 cccatcgcca gcaagggtgc ccgcctgacc acccagctca ccatcgcatc gcgctatctg
     1141 gtgctcatgc ccttcagcga ccacatcggg atttcccagc gagtcgacga ggaagacgaa
     1201 cgtgagcggt tgcgcttgct cgtcgacgag gtgcgcggcg ggcaagacgc cggcgtgggc
     1261 ttcatcgtgc gcaccgccgc ggatggcgtc gaccgggaac cgctggaagc cgacgtgcgc
     1321 gtcctcctgc atatctggga ccggatcatg gccaggcgag ccgagtgcgg ctgccccggg
     1381 atcgtttacg aggagttgcc gctgcacacc cgggtgattc gcgacatggc gacggcggag
     1441 ctggaatgca tacgaatcga tcacccgccg acgttggagc gggtgcggaa attcgtcgcc
     1501 gagttcctgc cggagttgac ggacaaggta gtgctctacg acgagccgag acccattttc
     1561 gagcgcttcg gcgttgacga cgagctgacc cgagcgctgg gcagccgcgt ggaactcaaa
     1621 tccggcggtc acctggtgat cgagcagacg gaagcgatga cgacggtgga cgtcaacacg
     1681 ggcgcctatc tcggcaagca caacctagag gaaaccgttt tccgcacgaa tctggaagca
     1741 gcccaggtgc tcccgcgaca gcttcgcctg cgcaacctcg gcggcatcat cgtcgtggac
     1801 ttcatcgaca tggaagacga agagcatcgc cgtcaggtgc tgcgcgcgct ggagaaggcc
     1861 tgcgagggcg acccggcgcg gattcgcatc gaggggttct catcgctcgg gctggtgcag
     1921 atgagccgga agcgcaccag ggagagcctg gttcagcagt tgtgcgagcc gtgctcgcac
     1981 tgtcacggcg ccggggtggt gaagacggcc cagagcacgt gcatcgagat tttccgctcg
     2041 atcatgcagg atgcggaaag cgggtgcggc gctcactcgc agggcgacta cgtcatccgg
     2101 gcagcggaca atgttgtaga caggttactg gacgaagacg ccgatcggct ggccgcgatg
     2161 gcggcgcgcg tgggtcgccg ggtgcgtatc gaattggaac caagctacgg cgcgggcgag
     2221 ttcgatatcg tcctcgtcgg cgatacgcag tgaaaatgtt gatctgaccg gacgaacggc
     2281 acggaaattt aaggtgaaat gcggggtcga aaggtagtag gggctgaacc gggccttgag
     2341 gccgattccg ggccgacttc cgtaaactaa tctcacatag gaccccatta cttgcaccga
     2401 atcgtactca ggccgttcct ggtcgtggtc acgctgacga tgctcttcgt cgcgctgatg
     2461 cagggcaccg ggcggttggg gttcatgttc ctgccggatc tcgaactggt cgcgaaccag
     2521 tggttgtccg gccagcgcgt tcgcgtctcg ggtctggaag gttcgtggcg ctacctcaat
     2581 ccggtggttc gagcggcgcg cgtggacctc cccgccggtc atctccggga agtcgtcgtg
     2641 gagctcgact ggttggaaag cctggtccgc aaccgcttcg tggcgcggcg ggtgctcgtc
     2701 ggggatggtg ccgtgcggct cgagaagacg ccggccggga aatggcgcct ggcggggaca
     2761 tcgggggatg gcgagttcga cgcgagcggc atcgtctatc acgccgacga gctgcgcttc
     2821 tccggcgtca tcgcttttgg ccttgccggt gaggaagagg cacagtcggt gcaggtggac
     2881 tacctggcca cgaaccgcgg tggcctgcat cgccatcgac tgcatctcga caaccaggac
     2941 gccgactgcg ccgcggactg cgggctccac ctggagtacc aggcccgcga cggccgctgg
     3001 ccgttctggg acgagcggat tcagctttcg ttggacggtg
//
LOCUS       DBNHOX010000369         4654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_736508, whole genome shotgun sequence.
ACCESSION   DBNHOX010000369 DBNHOX010000000
VERSION     DBNHOX010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_736508"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="AC16UZ_08120"
     CDS             complement(<1..307)
                     /locus_tag="AC16UZ_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6246686.1"
                     /translation="MPLREEKKRQVRKDILDAARELIEQRGYDSSTMRDIAGTARVSY
                     QTLYNYFPTKAAILAALLNDVVEQAVADIEALIEGYDGRLVETLNAINRARIDLVARR
                     "
     gene            380..1153
                     /locus_tag="AC16UZ_08125"
     CDS             380..1153
                     /locus_tag="AC16UZ_08125"
                     /inference="COORDINATES: protein motif:HMM:NF028441.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIR6246687.1"
                     /translation="MHSVKRLLLTAAHSMLVIFPAGVLAAEEPTARELVAAAIDLTRG
                     QSSYAEFAMVIHRPDWQRTSKLVAWTRGRKDALIRFTAPARDAGNATLKQGEKMWTYN
                     PKLNRTIRLPFSLMSQSWAGSDFSYNDLSRTDKLLEEYELAITATRRSEGHTLYTIEA
                     VPREEAPVVWGKEEIVLRDDHVLISQTFFDQAMRPLKRMETLEIGEMGGRIFGTRMRM
                     SKLDEPENYTELSYARVDFDIELEDRIFTLFSLQSGQRP"
     gene            1150..2418
                     /locus_tag="AC16UZ_08130"
     CDS             1150..2418
                     /locus_tag="AC16UZ_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6246688.1"
                     /translation="MTQARTPAAGGRARPALLLRIGWRNLARNRRRTWLTAGGIAFAV
                     LLVVFSMAMQLGQYDIMMENATSLLTGHIQIQTADYVDSARFEDTMGSAGALRRVIEA
                     TAGVEAVAPRAEAFALASAGERSFGARIIGVDPDAERDTVRLLKMVREGRAIAAPDDG
                     LLGSLLARNLGVTLGDEVVLLGSEKHGGVAAMVITVAGILETGVADLDRVTLITPLAS
                     VQSAFGLADEVHTFAIRTEDLDRSEAVAESLRHSLTDRPALVVRHWSEVMPELRQAIE
                     IDRLGGELFFYVVEVLVVFSIVNTFIMTVFERTREFGMLKAIGMKPWRIVAMMQWEGF
                     FIWLLGASLGLGLAAIIVAWLQDVGIYMGETLEEYAEQFYMPARLYPAFSLQAFLSAP
                     LVMLMGTQIAALLPSLRIFRLNAAAALRGE"
     gene            2507..3715
                     /locus_tag="AC16UZ_08135"
     CDS             2507..3715
                     /locus_tag="AC16UZ_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6246689.1"
                     /translation="MLKLLFQLSARNLLRHKRRNLMLLMAVVVAVGGVTLANALIRGF
                     QHDMREAAVQNLTGHLKVLAPGYLDDPGIQRSFTLVQGWSPDIGPRLLQGWAARIRIP
                     AVIMSERETRGVQFVGVDPAQETISFLGDASYRGEPLSGAADRRVVIGQALAEQLDTD
                     VGRRLVLITQGADGLNREAGFRIAGVYDADGQGLEKMFVFTGVTRLQELVDANVFTEV
                     SIKLREEPRGDGLKQGLAETFTDLDVMDWKELEPQTAAMFLFADTAIFIWFCIMMGAL
                     IFGLVNALITAVMERIREFGMLRAVGMRPGSVVVQVVLESTLIMAAGVALGLGLGFLL
                     VVWLSDGIDLTEWAAGVELFGMRSVLVPRLLLADVVLVAAMSIVFGLIASLYPAWRAV
                     QIGPLDALRR"
     gene            3723..4424
                     /locus_tag="AC16UZ_08140"
     CDS             3723..4424
                     /locus_tag="AC16UZ_08140"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6246690.1"
                     /translation="MAERVVRCIGVSRTYQDDSVPVHALRDVDFEVGKGEFVTLAGPS
                     GSGKSTLLNMIGGLDRPSSGCVEVDGVDLGSLSESRLSDLRLRKIGFVFQSYNLIPVL
                     SARENVEFIMQLQGVSAAERAARARAALEALGIGELEKRRPGELSGGQQQRVAVARAI
                     VTEPVLLLADEPSANLDSATTRELLEYLRRLNQDRGVTIVAATHDPMVMGYAQRQVGL
                     RDGAIVKDSADVAPE"
     gene            4408..>4654
                     /locus_tag="AC16UZ_08145"
     CDS             4408..>4654
                     /locus_tag="AC16UZ_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246691.1"
                     /translation="MWRRNRSWLIGPFLGLWATGSAAQSDFSARVKWFGTATALPEHD
                     LQRNLSGTPAYDQNYDLRLMFQKTISAVTLQVEHSTTL"
BASE COUNT          757 a         1410 c         1585 g          902 t
ORIGIN      
        1 cccgtcgcgc gacgagatcg atccgggccc ggttgatggc gttcagggtt tccaccagcc
       61 tgccatcgta gccctcgatg agcgcttcga tgtcggcgac cgcctgctcc acgacgtcgt
      121 tgagcagggc ggcgaggatg gccgccttgg ttggaaaata gttgtacagg gtctggtaac
      181 tcacccgtgc ggttccggca atgtcgcgca tcgtcgagct gtcgtatccg cgttgctcga
      241 tcagctctcg cgcagcgtct agtatgtcct tccggacctg tcgtttcttc tcttctctca
      301 gcggcatggg gacaataatt gttgatagag tcaaacgttg aggccgatcc ggatattgcc
      361 tgctcgtgga ggcttgtcaa tgcatagcgt caagcggctg ctgctcaccg ctgcgcactc
      421 gatgctcgtc atcttcccgg ccggcgttct cgccgccgaa gagcctactg ctcgggagct
      481 ggtcgccgcc gccatcgatc tcacccgggg gcaatcgtct tacgcggagt tcgccatggt
      541 cattcaccgg ccggattggc aacggacctc gaagctggtc gcctggaccc gcggccgcaa
      601 ggacgccctg atccggttca cggcgccggc cagggatgcg ggcaacgcga ccctcaagca
      661 gggcgagaag atgtggacct acaacccgaa gctgaaccgc acgatccggt tgcccttcag
      721 cctgatgtcg cagagctggg caggctcgga cttctcatac aacgatctct cccgcacgga
      781 caagctgttg gaagaatacg agctggccat cacggcgacc cggcgatccg agggccacac
      841 cctttacacc atcgaggcgg ttccccgcga agaggcgccg gtggtgtggg gcaaagagga
      901 gatcgtgctg cgcgacgacc acgttctgat ctcgcagacg ttcttcgatc aggccatgcg
      961 tccgctcaag cggatggaaa ccctggagat cggcgagatg ggcgggcgga tcttcggtac
     1021 ccgaatgcgc atgtccaagc tcgatgagcc ggaaaactac accgagctca gctacgcccg
     1081 ggtcgacttc gacatcgagc tggaagaccg aatcttcacg ctcttctcgc tgcagtcggg
     1141 ccagcggccg tgacccaggc gcggacgccg gcggccgggg ggcgtgcccg cccggcgctg
     1201 ctgctccgaa tcggttggcg caacctggcg cgcaaccgca ggcggacgtg gctgacggcg
     1261 ggtggcatcg cgtttgcggt gctgctggtg gttttctcca tggcgatgca gctcggtcag
     1321 tacgacatca tgatggagaa cgccacctcc ctgctgacgg gtcacatcca gattcaaacg
     1381 gccgactacg tcgacagcgc ccgcttcgag gacaccatgg ggagcgccgg tgcgctccgg
     1441 cgggtgatcg aagccaccgc cggcgttgag gccgtcgcgc cgcgcgcgga agcgttcgcg
     1501 ctggcttccg cgggagagcg gtcatttggt gcccggatca tcggcgtaga tcccgacgcc
     1561 gagcgcgata ccgtgcgcct gctgaagatg gttcgcgagg gtagagccat tgccgccccg
     1621 gatgacggcc tgctgggctc tttgctggcg cgcaacctcg gcgtgactct cggcgatgag
     1681 gtggtgctct tgggatctga gaagcacggc ggtgtcgccg ccatggtgat caccgtggcg
     1741 ggcatcctgg agaccggggt agccgatctg gatcgggtca ccctcatcac cccgctcgcg
     1801 tcagtccaga gcgcattcgg gcttgccgac gaggtgcaca ctttcgccat ccgcacggag
     1861 gatctggatc gcagcgaggc cgtcgccgag agtctgaggc acagtttgac cgaccggccc
     1921 gcgctcgtgg tcaggcattg gagcgaggta atgccggagc tgcggcaagc catcgagata
     1981 gaccggctcg ggggtgagct cttcttctac gtcgtcgagg tgctcgtcgt gttcagcatc
     2041 gtgaacacct tcatcatgac cgtcttcgag cggaccaggg aattcgggat gctcaaagcc
     2101 atcggcatga agccctggcg catcgtcgcg atgatgcagt gggaggggtt cttcatctgg
     2161 ctgctcgggg cttcgctggg gctgggcctc gccgccatca tcgtggcatg gcttcaggac
     2221 gtgggcatat acatgggcga gacgctcgag gagtatgccg agcagttcta catgccggcc
     2281 cggctttacc cggcgttttc gcttcaggcg tttctctccg cgccgctggt catgctgatg
     2341 ggaacccaga tcgccgcgct ccttccgtct ctgcgcattt tccggctgaa cgccgccgca
     2401 gcgctgagag gcgaatgatg tcgggtgacg ctgcttcgaa cgcgggcggt gcgatggctg
     2461 cccggaggac agggagggtg gtcgggtcgc cgcgccggtg tcgtccatgt tgaagcttct
     2521 ctttcagctc tctgctcgaa acctgcttcg gcacaagcgc cggaacctga tgctgctcat
     2581 ggcggtagtg gttgcggttg gcggcgtgac cctggccaac gcgctgattc gcggttttca
     2641 gcacgacatg agagaagccg ctgtgcagaa cctcaccggt catctgaaag tgcttgcgcc
     2701 cggctatctg gacgacccgg gaattcagag aagcttcact ctggtgcagg gctggtcgcc
     2761 cgacatcggc ccccggctgc ttcagggatg ggcggcacgc atcaggatcc cggccgtcat
     2821 catgagcgaa cgggaaaccc gcggcgtgca gttcgtcggc gtggacccgg cgcaggagac
     2881 gatctccttt ctgggggatg ccagctaccg cggcgagccg ctatccggtg cagccgatcg
     2941 gcgggtcgtg atcgggcagg cgctggccga gcagctcgat accgacgtgg gcaggcgcct
     3001 ggtgctgatc acacagggcg cggacgggct caatcgcgaa gccggattcc ggattgcggg
     3061 cgtttacgac gccgatggtc aggggctgga gaagatgttc gtcttcaccg gcgtgacgcg
     3121 ccttcaggag ctcgtggacg ccaacgtctt caccgaggtg tcgatcaagc tgcgcgaaga
     3181 gcctcgaggc gatggcctca agcaggggct ggcggaaacc tttaccgacc tggacgtgat
     3241 ggactggaag gaactcgaac cgcagacggc ggccatgttc ctgttcgcgg atacggcgat
     3301 cttcatctgg ttctgcatca tgatgggcgc tttgatattc gggctcgtca acgccctgat
     3361 caccgccgtg atggagcgga tcagagagtt cggcatgctg cgagccgtcg gcatgcgtcc
     3421 cggctcggtg gtggtgcagg tcgtgctgga atccaccctc atcatggccg ctggggtggc
     3481 cttggggctc ggcttgggtt ttctgctggt cgtgtggctg agcgacggaa tcgacctgac
     3541 ggaatgggcg gccggcgtgg agctcttcgg tatgcgcagc gtgctcgttc cgagacttct
     3601 cctcgcggac gtcgtgctgg tcgcggccat gagcattgtc tttggcctga ttgcgagtct
     3661 ctacccggca tggcgtgccg tgcagatcgg ccccctggat gctttgcgga gatgatgaag
     3721 aaatggccga acgggtagtg cgctgtattg gggtctcccg gacctaccag gacgattcgg
     3781 tgcccgtgca tgctcttcgg gatgtggact tcgaggtcgg caagggcgag ttcgtgacgc
     3841 tggcgggacc gtccggatcg ggaaagtcca cgttgctcaa catgatcggc gggctggacc
     3901 gccccagctc cggatgcgtc gaggtggatg gcgttgacct cggttcgcta agcgagagtc
     3961 ggctgtcgga tctgcggctg cgcaagatcg gcttcgtttt ccagtcctat aacctcattc
     4021 ccgttctgtc cgcaagggag aacgtggagt tcatcatgca gctgcagggc gtgtcggccg
     4081 cggagcgtgc cgctcgggct cgcgccgcgc tcgaggccct gggcatcggc gagctggaga
     4141 agcgacgtcc gggggagctg tccggaggtc agcagcagcg ggtggcggtt gcgagggcca
     4201 tcgtcactga gccggttctg ctgttggccg acgagccgag cgccaatctc gattcggcca
     4261 ccaccaggga gctgctcgag tatctgcgtc gcctcaacca ggaccgcggc gtgaccatcg
     4321 ttgccgccac ccacgatccc atggtcatgg gctacgctca gcgtcaggtc gggctgcgcg
     4381 acggcgcgat cgtgaaggac agcgccgatg tggcgccgga atagaagctg gctgatcggg
     4441 ccgtttctcg gtctgtgggc gacgggcagc gcggcgcagt cggacttttc cgctcgggtc
     4501 aaatggttcg gcacggctac cgccctgccg gagcacgatc ttcagcgcaa cctcagcggc
     4561 acccccgcct acgaccagaa ttacgacctg cgcctgatgt ttcagaagac gatctcggcg
     4621 gtaacgctgc aggtggagca ctcgaccacg ctgg
//
LOCUS       DBNHOX010000370         3405 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_93851, whole genome shotgun sequence.
ACCESSION   DBNHOX010000370 DBNHOX010000000
VERSION     DBNHOX010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_93851"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="AC16UZ_08150"
     CDS             <1..689
                     /locus_tag="AC16UZ_08150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6246692.1"
                     /translation="SCHLDFLTHGPALFAVPLFAAELSKVAERWGIDVHFQTNLIRVD
                     GPARQATFEKVGGEGEGEEVTMTFDMLHATPYQSPPAAVKESPLANEAGWVDVHQHSM
                     QHTTYANVFGLGDACSTPNSKTAAAVRKQAPVVVRNLLHLLDGGSVEEAYDGYGSCPL
                     TTAYGKVLLAEFTYGGKVTPTFPLDPRKERRSMWWVKKTALPIMYWDYMLRGHEAFPG
                     HDTEYEAPPV"
     gene            complement(706..1593)
                     /locus_tag="AC16UZ_08155"
     CDS             complement(706..1593)
                     /locus_tag="AC16UZ_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6246693.1"
                     /translation="MARWDRDSGASIPDWFFEAVETEYATRSVEVDECDVVYQLWGDE
                     DKPGILLIHGMNAHSHWWDFIAPQLLDGYRVAAMNLTGMGDSDYRYEYDGATYAQEIR
                     AVCDHAGFDERAALVAHSFGGYMAVKAAVLFPDRFGALVLVDSGIRHPEEPVPDRPAM
                     GGRSKVYPDRETALGRFRVQPPQSCDNAYILEYIARHSLMPVDGGWAWKFDEDLPSTL
                     KDVERTPEDYRHLDVPTGLIYGAESELFSARTEDYMRELIAQDFPAVALPEAQHHLFL
                     DQPLAFVAELRKMLATLLA"
     gene            complement(1597..2727)
                     /locus_tag="AC16UZ_08160"
     CDS             complement(1597..2727)
                     /locus_tag="AC16UZ_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6246694.1"
                     /translation="MDFSFSDEQTLLQDSIEKFIQNDYAFDARQRVVREEQGFSSDNW
                     RTFAELGWLGVPFPEADGGFGGTAIESMIMSEQFGKGLVVEPFLATVVLAGGALKIGG
                     SASQKEKYLAGVMDGSLQGALAYAEPQGRFNLADLTTTASPEGDGYVINGYKAVVMNG
                     PSADFLVVSCRTGGGQRDADGVSLFVLPADTEGVARRDYPTVDAFRASEVTLENVRVG
                     SDALIGEEGKGLAILEQVVDEAILAVGAEAVGCMEVLYKTTVEYCKTREQFGQPIGKF
                     QVLQHRMVDMFMEHEQAKSLMYMAAMRLDEGYGPEAQKAVSAFKVQVGKSGRFVGQNA
                     VQLHGGMGMTEELNVGHYFKRLTMIDTLFGNVDFHLKRFGSL"
     gene            complement(2737..>3405)
                     /locus_tag="AC16UZ_08165"
     CDS             complement(2737..>3405)
                     /locus_tag="AC16UZ_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6246695.1"
                     /translation="TSSTGKPQEGISFLLIDMRSPGVEVHPIEILGGQHSVNSVRLDG
                     VRVPVAHRVGEENQGWTYAKALLSHERTGVARVATSGVQLNRVREIAEGQEPSADGLW
                     QDAGFQRRYRELEIELIGLEMLELRILADLEAGQVPGPESSILKIKGTEIGQRINELM
                     VEALGYYALPYPDELLIDNEGPVGHDHALAALKGWLFSRAWSIYGGSNEVQKNIIARR
                     ALGL"
BASE COUNT          619 a         1147 c         1042 g          597 t
ORIGIN      
        1 gaagctgcca tctggacttt ctcacccacg gccccgctct gtttgcggtg ccgctgttcg
       61 cagcggagct gagcaaggtg gccgagcgct ggggcatcga cgtccatttt cagaccaacc
      121 tgatcagggt ggacgggccc gcacggcagg ccaccttcga aaaggtcggc ggcgaaggag
      181 agggagaaga ggtcaccatg accttcgaca tgctgcacgc aaccccgtat cagagcccgc
      241 ccgcggccgt gaaggagagc cccctggcca acgaggcagg ctgggtcgac gtgcaccagc
      301 acagcatgca gcacaccacc tacgccaacg ttttcgggct gggggacgcc tgctcgaccc
      361 caaactccaa gacggcggct gccgtgcgca agcaggcgcc ggtggtggtg cgcaatctgc
      421 tccacctgct cgacggcggc agcgtcgagg aagcttacga cggctacggc tcgtgccccc
      481 tgaccaccgc gtacggcaag gtcctgctgg ccgagttcac ctacggggga aaggttacgc
      541 cgaccttccc gctggacccg cgcaaggagc gaagaagcat gtggtgggtg aagaagacgg
      601 cgctgccgat catgtactgg gactacatgc tgcgaggtca cgaggcgttc ccgggccacg
      661 acacggagta cgaagcgccg ccggtgtgag cgggctaggg cgagttcagg cgagaagcgt
      721 cgccagcatc ttcctcagct cggcgacgaa cgccagcggc tggtccagga acagatggtg
      781 ctgtgcttcc ggcagcgcca cggccgggaa gtcctgggca atgagctcgc gcatgtagtc
      841 ttccgtgcga gccgagaaca gctcgctttc cgctccgtag atgaggcccg tgggcacgtc
      901 cagatgccgg tagtcttccg gcgtgcgctc gacatccttt agagtcgacg gcaggtcctc
      961 gtcgaacttc caggcccatc cgccatccac gggcatgagc gaatgacgtg cgatgtactc
     1021 gagaatatag gcgttgtcgc agctctgcgg gggctgcacg cgaaagcggc cgagcgcggt
     1081 ctcccggtcg ggatacacct ttgaccgccc tcccatcgcc ggcctgtccg gaaccggctc
     1141 ctccggatga cggattccgg aatccaccag gaccagtgcc ccgaaacgat cgggaaagag
     1201 cacggccgct ttgacggcca tgtaaccgcc gaagctgtgc gccaccaggg ctgcacgctc
     1261 gtcgaacccc gcatggtcac agacagccct gatctcctgg gcgtaggtgg cgccgtcgta
     1321 ctcgtagcgg tagtcggagt cgcccatgcc cgtgaggttc atggcggcga cccgatagcc
     1381 gtcgagcagc tgaggcgcga tgaagtccca ccagtgggag tgggcgttca tgccgtggat
     1441 cagcaggatc ccgggtttat cctcgtcgcc ccatagctgg tacaccacgt cgcactcatc
     1501 gacctcgact gaccgggtcg cgtactcggt ctccaccgcc tcgaagaacc agtcgggtat
     1561 ggaggccccg gagtcacgat cccagcgagc cacgatctaa agcgacccga accgcttgag
     1621 gtggaagtcc acgttgccga acagcgtgtc gatcatggtg aggcgcttga aatagtggcc
     1681 tacgttgagc tcctcggtca ttcccatccc gccgtgcagc tggacggcgt tctggccgac
     1741 gaacctgccc gacttgccca cctgaacctt gaacgccgat acggctttct gcgcttccgg
     1801 gccatagccc tcgtccaggc gcatggccgc catgtacatc agagacttgg cctgctcgtg
     1861 ctccatgaac atgtccacca tgcgatgctg cagcacctga aacttgccga tgggctgacc
     1921 gaactgctcg cgggttttgc agtactccac cgtcgtcttg tagagcacct ccatgcagcc
     1981 cacggcttcc gcgccgacgg ccaggatggc ctcgtcgacg acctgctcga gaatggcgag
     2041 cccctttcct tcctcgccga tcaacgcatc gcttcccacc cggacgttct ccagcgtcac
     2101 ctcggacgcc cggaaggcat ctaccgtggg gtagtcgcgc ctggccacgc cttcggtatc
     2161 cgcaggcagc acgaagaggc tgacgccatc cgcatcccgc tggccgccgc ccgtgcggca
     2221 ggatacgacc agaaagtcgg cggacgggcc gttcataacc acggctttgt aaccgttgat
     2281 cacgtagcca tcaccctccg gggacgccgt ggtggtgaga tcggcaaggt tgaatcgtcc
     2341 ctggggctcc gcgtacgcca gggcaccctg cagcgatccg tccatgacgc cggcgaggta
     2401 tttctccttc tgcgacgcgc tgccgccgat cttcagcgcg cccccggcga gaaccaccgt
     2461 cgccaggaag ggctccacga ccagcccttt gccgaactgc tccgacatga tcatcgactc
     2521 gatggccgtt ccgccgaagc cgccgtcggc ttccgggaac gggaccccga gccagccgag
     2581 ctcggcaaac gtccgccagt tgtcgctgga gaaaccctgc tcctcccgaa ccacccgctg
     2641 gcgcgcgtcg aacgcgtagt cgttctggat gaacttctcg atgctgtcct gcagcagcgt
     2701 ctgctcgtcg gaaaaagaaa aatccataac ttcccctcaa agacccagcg cccgccgggc
     2761 gatgatgttt ttctgaactt cgttcgaccc gccgtaaatg ctccaggcgc ggctgaacag
     2821 ccaacccttg agcgcggcga gcgcgtgatc gtgacctacc gggccttcgt tgtcgatcag
     2881 caactcatcg ggatagggca gcgcgtagta gccgagggcc tccaccatca actcgttgat
     2941 gcgctggccg atctccgtgc ccttgatctt gagaatggac gactccggcc ccgggacctg
     3001 gcccgcctcg aggtctgcaa gaatgcgcaa ctccagcatc tcgaggccaa tcagctcgat
     3061 ttcgagttcc cgatagcgac gctggaagcc tgcgtcttgc cagagaccgt ctgcgctcgg
     3121 ttcctgccct tcggcaatct cccgtacgcg attgagctgc actccagacg tcgcgacccg
     3181 ggccacccct gtccgctcat ggctcagaag agctttggcg taggtccagc cctggttttc
     3241 ctcgcccaca cgatgcgcga ccgggacgcg gacaccgtcc agccggacgc tgttcacgct
     3301 gtgctggccg ccgaggatct cgatgggatg aacctcgaca cccggcgagc gcatgtcgat
     3361 gagcaggaag ctgatgccct cctggggctt gcccgtcgaa gaggt
//
LOCUS       DBNHOX010000371         4071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1122682, whole genome shotgun sequence.
ACCESSION   DBNHOX010000371 DBNHOX010000000
VERSION     DBNHOX010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1122682"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(348..629)
                     /locus_tag="AC16UZ_08170"
     CDS             complement(348..629)
                     /locus_tag="AC16UZ_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR6246696.1"
                     /translation="MTMTIKRAMGAALLLAGVLAGPVLAAPESVNINSANAETLAEVL
                     NGVGLTKAEAIVEYREQHGAFRDAYELANIKGIGERTVEKNEARIRLQD"
     gene            complement(755..1927)
                     /gene="lapB"
                     /locus_tag="AC16UZ_08175"
     CDS             complement(755..1927)
                     /gene="lapB"
                     /locus_tag="AC16UZ_08175"
                     /inference="COORDINATES: protein motif:HMM:NF008757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HIR6246697.1"
                     /translation="MPENLVVYLLLVLALGIGFLLGRRERKRKQSSPGMVAQEYFRGL
                     NHLLNERHDLAIDTFVEAMAVDSDTVDNHLALGSLVRRRGEVDRAIRIHQNLLARPVL
                     SPAHRAQTELELARDYLSAGLLDRAENLLLELVKKPGDDRDVARTMLLEIYQREREWE
                     RAVEVARELSRADRSYRTPLAHFQCELAEKALADGDLRRARADLERAGDFDPGCARVA
                     LVGAKVASAGGRHRDVKRLLRKARSLEPELSGETVAMFRAASEELGDRADFVRYLREC
                     LERAAPLEVVQALTREIEADEGDEVAREFLIEQLLRNPNLAGFVSLLEQLDRGGAALE
                     ADQLALVRRFSSSLLQKQPSHRCKDCGFSGHTLIWQCPSCKTWGSIKPIVRYDGDV"
     gene            complement(1934..2227)
                     /locus_tag="AC16UZ_08180"
     CDS             complement(1934..2227)
                     /locus_tag="AC16UZ_08180"
                     /inference="COORDINATES: protein motif:HMM:NF018058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIR6246698.1"
                     /translation="MAWLSRFFWALLALLVFCLAGLAVNQEPISLKFVTWQTPQLSVF
                     WWLLAAFSTGVLLGLVGISVVRARLGLKNRSLVKRLEASERELRRLRSVSYHE"
     gene            complement(2237..2563)
                     /gene="ihfB"
                     /locus_tag="AC16UZ_08185"
     CDS             complement(2237..2563)
                     /gene="ihfB"
                     /locus_tag="AC16UZ_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HIR6246699.1"
                     /translation="MTKSELIERIAARQTQLSAKDVELAVKTLIEQMSETLAAGHRIE
                     IRGFGSFSLHYRAPRIGRNPKTGESVGLAGKYVPHFKPGKELRDRVNQGLERERAEQE
                     SAGLPS"
     gene            complement(2684..>4071)
                     /gene="rpsA"
                     /locus_tag="AC16UZ_08190"
     CDS             complement(2684..>4071)
                     /gene="rpsA"
                     /locus_tag="AC16UZ_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR6246700.1"
                     /translation="DAFNKGEVITGAINGKVKGGFTVDISDIRAFLPGSLVDIRPLRE
                     TTHLEGKPLEFKVIKLDQKRNNVVVSRRAVLEQENSAEREALLESLQEGQEVKGIVKN
                     LTDYGAFVDLGGVDGLLHITDMAWRRIRHPSEMVNVGDEVNVKILKFDREKNRVSLGM
                     KQLGDDPWVELTRRYPEGSRVRATVTNLTDYGCFAEIEEGVEGLVHVSEMDWTNKNIH
                     PSKVVNVGDEVEVMVLDIDEERRRISLGIKQCRANPWEEFSAHHAKSDRIKGSIKSIT
                     DFGIFIGLDGGIDGLVHLSDISWNEPGETAVRRYSKGEEIETVILSVDPERERISLGI
                     KQLDEDPFTDYTAVNDRGSVVTGVIVEVEPKEAVIRLAEDVTGVLKASEISIDRIEDA
                     RNVLTAGEEVEVKIISVDRKNRNLALSIKAKAVDDEREAVREHRRQESERVGPSTLGD
                     LIKAQMDQEES"
BASE COUNT          705 a         1378 c         1205 g          783 t
ORIGIN      
        1 gtgagagaga aagaaccgtc cttcgttgac cacgtagatg atcttcatca cccgatcatc
       61 catatgagca ggtcggcgcc ggtacccgcg ccggcagagg tgggcattag gggcaaacgc
      121 tgcaaagaat ccatacttcg tcgcgacgcc atcggcggct ttcgtgctcc atcttaccgc
      181 ccgcgccccg gcaatgagag gtgctccgga caatgcgctt gaccggcgtc acaaaaacac
      241 cgacgagcgg ccaggctccg gggcaagcct gccacccgga gcctggcctg gcgaactcag
      301 gacgacgccc ggcgaacgcc gcatcgaagg tgcctcaagc cgcagggtca atcctgcagg
      361 cggatccgcg cctcgttctt ctcgacggtc cgttcgccga tgcccttgat gttcgcaagc
      421 tcatatgcgt cgcggaacgc gccgtgctgc tcacggtact cgacgatggc ctcggccttg
      481 gtgagcccaa cgccgttcag cacctcggca agcgtctccg cattcgcgct gttgatgttc
      541 acactctccg gggccgcgag caccggcccg gcgagcacac cggccagcag cagcgcagcg
      601 cccatcgcac gtttgatcgt catcgtcatg ggtaactccg ttttcagtgg tgaggaggac
      661 gataatgcta cgaggcgccc aggcgtccgc gtaagccgca aagagccgcc gtttccgtaa
      721 gttcggaccc ggcgaggacg cgggccaatg gagttcagac gtcgccgtcg taacgcacga
      781 tgggcttgat gctgccccag gtcttgcagc tcggacactg ccagatgagc gtgtgcccgc
      841 tgaagccaca gtccttgcag cgatggctcg gctgcttctg gagcagcgag ctcgagaacc
      901 tccgaaccag cgccagctgg tcggcctcga gagccgctcc gccacggtcg agctgctcga
      961 gcagggagac gaagcccgcc agattggggt tgcgcagcag ctgctcgatc agaaactccc
     1021 gcgccacctc gtcgccttcg tcagcctcga tctcccgggt gagcgcctga acgacctcca
     1081 gcggggccgc cctctccaga cactccctca gatagcgcac gaaatcggca cgatccccga
     1141 gctcttcact ggcggcccgg aacatcgcca cggtttcccc gctgagctcg ggctcgaggc
     1201 tgcgagcctt ccgcagcagc cgcttgacgt cgcggtggcg gccaccggcg gaggcgactt
     1261 tcgcgccgac cagggcgacc cgagcgcaac ccggatcaaa gtccccggct cgctcgagat
     1321 cggcgcgggc gcgccgcaga tccccatcgg ccagagcctt ctccgcaagt tcgcactgaa
     1381 agtgagccag cggcgtgcga taggatcgat cggcacggga gagctcccgg gccacctcca
     1441 cggcgcgctc ccactcccgc tcccgctgat agatctcgag cagcatcgtg cgcgccacgt
     1501 cgcgatcgtc gccgggtttc ttgaccagtt ccagcagcag attttccgcc cggtccagca
     1561 gcccggcgga cagatagtcg cgcgccagct cgagctcggt ctgagcgcgg tgcgcaggag
     1621 aaaggaccgg ccgggcgagc aggttctggt gaatgcggat ggctcggtcc acctctccac
     1681 gtcgccggac gagacttccg agcgccaggt ggttgtcgac ggtgtcgctg tccactgcca
     1741 tggcttccac gaacgtgtcg atggccaggt catgacgctc gttgaggaga tggttcaggc
     1801 cccggaagta ctcctgagcc accatgccgg gactggactg tttgcgtttg cgctcgcgtc
     1861 gcccgagaag aaagccgatg ccgagtgcca gcacgagaag caggtagacg acaaggttct
     1921 cgggcatgcg cgtttactcg tggtaggaca cgctgcgcaa tctgcgcagc tcacgttcgc
     1981 ttgcctcgag ccgtttaacc agcgaccggt tcttcagccc gagccgggcc cgaacgaccg
     2041 agatgccaac cagacccaac agcacaccgg tgctgaacgc ggctagcagc caccagaata
     2101 cgcttagttg cggggtctgc caggtgacga acttgagcga aatcggttcc tgattgaccg
     2161 ccagccctgc caggcagaac accagcagcg caagcagcgc ccagaagaat cgtgacaacc
     2221 aagccatggg cttaggctag ctcggaagcc cggcggactc ctgctccgct cgttcccgct
     2281 ccagaccctg gttcacccga tctcgcagct ccttgcccgg cttgaagtgg ggaacgtact
     2341 tgcccgccag ccccacggac tcgccggtct ttggattacg gccgattcgc ggcgcccggt
     2401 agtgcagcga aaagctgccg aagcctctga tctcgatccg gtgtcccgca gcaagcgttt
     2461 cagacatctg ctcgatgagc gtctttacgg cgagctcgac gtccttcgca gacagctgcg
     2521 tctgcctggc cgcgattcgc tcgatcagtt ccgatttggt catgtctgcc tgcttgtcag
     2581 gcccgaccta cgcccgattg accggcgtct tccggacccc ctcccccgtt catcgcgttg
     2641 tcacgggcgg tcctttgatc gggccgcccg ctctttttga aggctagctc tcttcctgat
     2701 ccatctgcgc cttgatgaga tctccaagcg tgctcggtcc cacgcgctcg ctttcctggc
     2761 gccggtgctc cctcaccgcc tcgcgctcgt cgtccaccgc tttggccttg attgacaggg
     2821 caagattgcg gttcttgcgg tccacgctga tgatcttgac ctccacctct tcacccgccg
     2881 tcagcacgtt gcgagcatcc tctatgcggt cgatgctgat ctccgacgcc ttgagaacac
     2941 cggtcacgtc ttcggctaag cgaatgacgg cttccttggg ttcgacttcc acaatcacgc
     3001 ccgtgaccac cgagccgcga tcgttgaccg cggtgtagtc ggtgaacgga tcctcgtcga
     3061 gctgcttgat gcccagggag attcgctcac gctcgggatc gacggacaga atgaccgtct
     3121 cgatctcctc acccttgctg taacgacgca cggcggtttc gccgggctcg ttccaggaga
     3181 tgtcggaaag gtgcaccaga ccatcgatgc cgccatccag accgatgaag atcccgaagt
     3241 cggtgatgga cttgatgctg cccttgatcc gatcactctt ggcgtggtgg gcgctgaact
     3301 cctcccacgg gttcgcacgg cactgcttga tgcccagcga gatgcgacgg cgttcctcgt
     3361 cgatgtccag gaccatcacc tcgacctcat cgcccacgtt gacgaccttg gaggggtgga
     3421 tgttcttgtt ggtccaatcc atctcggaaa cgtgcacgag accctcgaca ccttcctcga
     3481 tctcggcaaa acagccgtaa tcggtaaggt tggtgaccgt ggcccggacc cgcgaacctt
     3541 ccggatagcg ccgggtcagc tccacccacg gatcgtcccc cagctgcttc atgccgagac
     3601 tgacgcggtt cttctccctg tcgaacttca gaatcttgac gttgacctcg tcgcctacgt
     3661 tgaccatttc gctgggatgt cgaatccggc gccaggccat gtcggtgatg tgcagcagcc
     3721 cgtctacgcc gccgaggtcc acgaacgcgc cgtagtccgt gaggttcttc acgatgcctt
     3781 tgacttcctg accttcctgc agggattcca ggagcgcctc gcgctcggca ctgttctcct
     3841 gctctagcac ggctcgccgt gacaccacga cgttgttccg cttctggtcg agcttgatca
     3901 ccttgaactc gaggggcttg ccctcgagat gggtggtctc gcgcagcggc ctgatgtcca
     3961 ccagggaccc gggaaggaac gcccggatgt cgctgatgtc caccgtgaac ccacccttga
     4021 ccttgccgtt gatggcaccg gtgatgacct cgcccttgtt gaaggcgtcc t
//
LOCUS       DBNHOX010000372         1683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1186420, whole genome shotgun sequence.
ACCESSION   DBNHOX010000372 DBNHOX010000000
VERSION     DBNHOX010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1186420"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="AC16UZ_08195"
     CDS             <1..722
                     /locus_tag="AC16UZ_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HIR6246701.1"
                     /translation="YATYRHVNDSRELQARLTYSNAALLEMMRVHLVEEDPQDEPVAR
                     SAQAPAATGAQTETFRIGEDGAALMPYETLINPPSVASDALHWPWRTVKANLDRLEAL
                     GADYVGRRLYLLFNPRTERFNGTTPNFFATITIRPPGIVDKPHRHVSSAINYYFNGSG
                     YSRVEGKRYDWKAGDLMVSAPGWAVHNHASNEGDPVYELTIQDQPLNIYMESLLWQED
                     LAEPARILGSHEGFSTNRSTL"
     gene            743..1363
                     /locus_tag="AC16UZ_08200"
     CDS             743..1363
                     /locus_tag="AC16UZ_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR6246702.1"
                     /translation="MFYDPREDPQPEPLGHNPINALVAPRPIGWIASLSADGAPNLAP
                     FSYFNAFSADPPVVAFAPNEKSGAGGPKDTLANVRSVPEFTASLVSEPLARRMNESSR
                     VLPHGVSEFIEAGLTPAASRLVAPPYVGEALAALECRVFDIIELPGRPNGRRSHLVIG
                     EVVGIHIDDGVIVDGRIDSLALAQLARLGYHDYTVVRELIEIPRPD"
     gene            1365..>1683
                     /locus_tag="AC16UZ_08205"
     CDS             1365..>1683
                     /locus_tag="AC16UZ_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6246703.1"
                     /translation="MAPSTSDPDRSYGYQSRATHRAFDRLLQQHLRQHGLTNGYWYVL
                     RVLWEEEGMSQRELARASNLTESSAVIMLDGMQRAGLVARRKDPADGRRQRVFLTAKA
                     KRLK"
BASE COUNT          307 a          585 c          527 g          264 t
ORIGIN      
        1 cctacgccac ctaccggcac gtcaacgaca gccgcgagct ccaggcaagg ctgacctact
       61 ctaacgccgc tctgctggag atgatgcgcg tgcatctggt ggaggaggac ccgcaggacg
      121 agccggtggc aaggagcgcc caggcgcccg cggccaccgg ggcgcaaacg gagaccttcc
      181 gcatcggtga ggacggcgcg gcgctgatgc cctacgagac cctcatcaac ccgccgtcgg
      241 tagcctccga cgcgctgcac tggccctggc ggacggtgaa agcaaatctg gacaggctgg
      301 aagcgctggg agccgactac gtgggacgcc gcctctacct gctgttcaat ccccgaaccg
      361 agaggttcaa cggcaccacg ccgaacttct ttgccaccat cacgattcga ccgccgggaa
      421 tcgtggacaa accccatcgc cacgtgtcct ccgccatcaa ctattacttc aacggttcgg
      481 gttacagccg cgtcgagggc aaacgctacg actggaaggc aggtgatctc atggtttccg
      541 cgccgggctg ggccgtgcac aaccacgcct ccaacgaagg cgacccggtg tacgagctga
      601 ccattcagga tcagccgctg aacatctaca tggagtcgct gctgtggcag gaggacctgg
      661 ccgagcctgc ccgaatactc ggatctcacg agggattctc caccaaccgt tcgacgctct
      721 gatcgctgct gacgcccgaa acatgtttta cgatccgcga gaagatcccc agcccgagcc
      781 gctgggccac aatccgatca acgccctcgt cgcgccaagg cccatcgggt ggatcgcctc
      841 cctctccgcc gacggcgcgc cgaatctggc accgttctcc tacttcaacg cgttctcggc
      901 agacccgccc gtcgtggcct tcgcccccaa cgagaagtcc ggcgcggggg gtcccaagga
      961 tacgctggcc aacgtcagga gcgtgcccga gttcaccgcg agcctcgtct ccgaacctct
     1021 ggcccggcgg atgaacgaat cgtcccgcgt gctgccccac ggcgtcagcg agttcatcga
     1081 agccggattg accccggcgg cgtcgcgcct ggtggccccg ccgtacgtgg gcgaagcgct
     1141 ggccgcgctg gaatgccgcg tgttcgacat catcgagctg ccgggccgcc cgaacggacg
     1201 ccgcagccac ctggtcatcg gcgaggtggt tgggatccac atcgacgacg gggtgatcgt
     1261 cgacgggcgc atcgactctc tcgccctcgc tcagctcgcc cgcctgggtt accacgacta
     1321 caccgtggtg cgggagctga tcgaaattcc gaggcccgac tgagatggcg ccgtctacca
     1381 gcgacccgga tcgcagctac ggttaccagt cgcgggccac ccaccgcgcc ttcgaccggc
     1441 tgctgcagca gcacctgcgc cagcacggtc tcaccaacgg ctactggtac gtgctgcgcg
     1501 tgttgtggga ggaggagggc atgtcacagc gggagctggc tcgagcgtcc aacctgaccg
     1561 agtcctccgc ggtgatcatg ctggacggca tgcagcgcgc gggcctggtg gcgcgcagaa
     1621 aggatcctgc ggatggtcgg cgccagcgcg tcttcctgac ggcaaaggcg aaacgactga
     1681 aac
//
LOCUS       DBNHOX010000373         3493 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_93955, whole genome shotgun sequence.
ACCESSION   DBNHOX010000373 DBNHOX010000000
VERSION     DBNHOX010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_93955"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="AC16UZ_08210"
     CDS             <1..442
                     /locus_tag="AC16UZ_08210"
                     /inference="COORDINATES: protein motif:HMM:NF021438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HIR6246704.1"
                     /translation="TPVASANQTVDTDAKASRVEGVWASKGAVFAIERSGDTLQATVV
                     ALRKPRPDRKNAKAELRDRPLIGLQLLSDYRFKRGRWRGDFYDPGSGRTYSSYIRLDD
                     EGNLRLRAWVLMFGHTEVMEPVGNCTERIIAMLEVSGVAGLCPP"
     gene            462..1598
                     /locus_tag="AC16UZ_08215"
     CDS             462..1598
                     /locus_tag="AC16UZ_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HIR6246705.1"
                     /translation="MSVVPVSGRSGLNDFIEVPWVVYADDPCWVPPLKMERRDALSPK
                     HPFFEHARWQPFVAYRDGRAVGRISAQIDELYQQRHDPKGGFFGMFESIPDDAVIDGL
                     LASAEAWLAERGCEHVVGPFNLNITQEVGLLVDGFDSSPFFMMGHGMPYYGEALARRG
                     YAEVQRMLAYEMPVLFERPRLMSIMMKQLGPRVTMRTLDRRKLVEELEILRDIFNDAW
                     SENWKFVPWTSREFLAVGKEMMMLIPPDLVQIAEVDGEPAAFIVMLPNLNEVIGDLDG
                     RLLPFGWAKLLWRIKVRYPKSGRVPLMGVRRRFHRTRLGPGLAMFCISALREPAHRAG
                     LERAELSWILEDNQGMRSILEAVGGEVTRTYAMFEKSLSRGPGS"
     gene            1595..2386
                     /locus_tag="AC16UZ_08220"
     CDS             1595..2386
                     /locus_tag="AC16UZ_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR6246706.1"
                     /translation="MSEVSVCGAVVLAGQRAGPTPLCAQAGVDVEVLVPVGGVPAIER
                     VLGALSRAELVRPAVVVGPSPEVLSAHPEVKAMIDAAGAAWVAPAGDPASSALAGLQG
                     QAPPTLVTSGDHALLCASIVDAFCEAAHRVQADLIVGLVPYQAVRAAYPESRRTVLKF
                     ADGARCGANLYFLRTEEARRGIEFWVAVQRDRKRPWRIARRLGWRLLLRYLTGRLTLG
                     AALGALSERAGCRLGSVIVDEPRAAVDVDSVDDWRLAQRIVGEAS"
     gene            2383..3123
                     /locus_tag="AC16UZ_08225"
     CDS             2383..3123
                     /locus_tag="AC16UZ_08225"
                     /inference="COORDINATES: protein motif:HMM:NF016974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HIR6246707.1"
                     /translation="MSGTASLPRRWVIKGGKRLLRAIGRFQGRHSLVGTGPFLENAEF
                     PWVPSLEGRWEEIRSELATVMEHPEDIPAFHQLSPDQKRISKGDNWKTFAFYVFGIRL
                     EENCRRCPVTAATIECLPGLQNAWFSILAPRYHIPPHKGPTRAVIRCHLGLAVPRDGE
                     RCWLRVDDQIRHWEAGGCLLFDDTFEHEVRNDTDETRIVLFLDVDRPSDRLGTWFNRL
                     IVTLIRGSAYVKDPLKNLADWNRARREA"
     gene            3126..>3493
                     /locus_tag="AC16UZ_08230"
     CDS             3126..>3493
                     /locus_tag="AC16UZ_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6246708.1"
                     /translation="MLLDLEKLPQGERFQADVCIAGAGAAGVALARRLAELGHSICLL
                     ESGGLDFEQSTQDLYAGTSDGMTYYDLVDARLRFLGGTTNIWGGRCALLDDIDFRRRP
                     WVPHSGWPITNAELTPYYAAV"
BASE COUNT          541 a         1122 c         1195 g          635 t
ORIGIN      
        1 gacacccgtc gcctcggcca accaaaccgt agacacggac gccaaagcca gccgcgtaga
       61 gggggtctgg gcctccaagg gtgccgtgtt tgccatcgag cgctccgggg acaccctgca
      121 agccaccgtc gtcgctctgc gcaaaccccg acccgaccgc aaaaacgcta aagccgagct
      181 gcgcgaccgg ccgctgatcg ggctgcagct gttgagcgac tatcgtttca agaggggccg
      241 ctggcgcggg gatttctacg acccggggtc aggcaggacc tattccagtt acatccggct
      301 ggatgatgag ggcaacctta ggcttcgcgc ctgggtgctg atgttcggtc acaccgaagt
      361 gatggagccg gtcggcaact gcaccgaaag gatcatcgcc atgctcgaag tgtcgggggt
      421 cgccggcttg tgtcccccat aacgttcgct tcggcatgtc attgagcgtc gtacccgtgt
      481 ccggacggtc cgggcttaac gacttcatcg aggtgccctg ggtcgtgtat gcggatgacc
      541 cgtgctgggt tccgccgctg aagatggagc gccgggacgc gttgtccccc aagcatccct
      601 tcttcgagca cgcccgctgg cagccgttcg tcgcgtaccg cgacggccgg gccgtcggcc
      661 gcatctccgc ccagatcgac gagctgtatc agcaacgcca cgaccccaag ggcgggttct
      721 tcggcatgtt cgaaagcatc ccggacgacg ccgtcatcga cggcctgctc gcaagcgccg
      781 aggcgtggct ggccgagcgt ggctgcgagc acgtggtggg tccgttcaac ctgaacatca
      841 cccaggaggt gggcctgctc gttgacggct tcgattcgtc gcccttcttc atgatggggc
      901 atggaatgcc ctattacggc gaagcgctgg cgcggcgtgg ttacgccgag gtgcagcgca
      961 tgcttgccta cgagatgccc gtgctcttcg aaaggccgcg gctgatgagc atcatgatga
     1021 agcagttggg cccgcgggtg acaatgcgca cgctcgaccg gcgcaagctc gtcgaagagc
     1081 tcgaaatact gcgcgacatc ttcaatgatg cctggtccga gaactggaag ttcgtcccct
     1141 ggacgtccag agagtttctt gccgtgggta aggaaatgat gatgctgatc ccgccggatc
     1201 tcgtgcagat agccgaggtc gacggcgagc cggcagcgtt catcgtcatg ctgccgaacc
     1261 tcaacgaagt catcggtgat ctcgacggcc ggctgcttcc gttcggctgg gccaagctgc
     1321 tctggcgcat caaggtccgc tatccgaagt ccggccgggt gccgctcatg ggcgttcgcc
     1381 ggcgctttca ccgcacgcgg ctcggtccgg gcctggccat gttctgcatc agcgccctgc
     1441 gtgagcccgc ccatcgtgcc ggtctcgagc gggcggaact ttcttggatc ctggaagaca
     1501 atcagggcat gcgcagcatt ctcgaggcgg tcggggggga agtcacccgg acctatgcca
     1561 tgttcgagaa gtcgctttct cgaggccccg ggtcgtgagc gaggtgagtg tttgcggcgc
     1621 ggtggtgctc gccggccagc gtgccggccc gacgcctttg tgcgcccagg cgggcgtcga
     1681 cgtagaagtt ctcgttccgg tgggcggcgt gccggccatc gagcgcgttc tgggagccct
     1741 gtcgcgcgcg gaactcgtgc gcccggctgt ggtggtgggc ccgtcccccg aggtgctgtc
     1801 ggcccatcct gaggttaagg caatgatcga cgctgccggg gcggcctggg ttgctccggc
     1861 cggcgacccg gcctccagcg cacttgccgg cctgcaaggc caggcaccgc cgacgctggt
     1921 caccagcggc gatcacgcgt tgctctgcgc atccatcgtc gacgctttct gcgaggctgc
     1981 gcaccgggtg caggcggacc tgatcgtcgg cctggttccc taccaggcgg tgcgggccgc
     2041 ctatccggaa agccgcagaa cggtgctcaa gttcgccgac ggtgccagat gcggcgccaa
     2101 cctctatttt ctgcgcaccg aggaggcgcg gcggggcatc gagttctggg tggccgtgca
     2161 gagggatcgc aagcgtccct ggcgcatcgc gcgtcggctc ggatggcgcc tgctgctgcg
     2221 ctacctgacg ggacggctga ctctgggggc cgcgctcggg gcgttgtcgg agcgcgccgg
     2281 ctgccggttg ggatcggtga tcgtggacga gccgcgcgcc gcggtggatg tggacagcgt
     2341 cgacgactgg cggctcgcgc agcggatcgt cggagaggcg tcgtgagcgg gaccgcttcg
     2401 cttcccaggc gctgggtgat caagggcggc aaacgtctgc tgcgggccat cggccggttc
     2461 cagggccgcc actctctggt gggcacggga ccgtttctgg agaatgccga atttccctgg
     2521 gtaccctcgc tggaggggcg ctgggaggag atccgatccg agctggctac ggtcatggaa
     2581 cacccggagg acatccccgc gtttcatcag ctgtcgccgg accagaagcg catctccaag
     2641 ggcgacaact ggaagacgtt cgccttctac gtgttcggta tccgcctgga agaaaactgc
     2701 cggcgatgcc cggtcacggc tgcgaccatc gaatgccttc cgggtctgca gaacgcctgg
     2761 ttctccatcc tcgcgccccg ctaccacatc ccgccgcaca aggggcctac ccgtgcggtg
     2821 attcgttgcc acctgggtct tgccgtgccc cgtgacggag aacggtgctg gctgcgcgtc
     2881 gacgaccaga tccgccactg ggaggcgggc ggctgtctgc ttttcgatga tacgttcgag
     2941 cacgaggtgc gaaacgatac cgacgagacg cgcatcgtgc tgtttctgga tgtggaccgg
     3001 ccgtcggacc gcctgggcac gtggttcaac cgactgatcg tcacgctcat ccgcggcagc
     3061 gcctacgtga aggatccgct gaagaacctg gccgactgga accgggcgag gcgcgaggct
     3121 tagtcgtgct gctcgatctc gaaaagctgc cccagggcga gcgtttccag gccgacgtct
     3181 gcattgccgg cgccggcgca gccggtgtgg cgctggcccg gcgcctggcg gagctcggtc
     3241 actccatctg cctgctggag agcggcggcc tggatttcga gcagtccacc caggatctct
     3301 acgccgggac cagcgacggc atgacctact atgacctggt cgatgcccgc ctgcgctttc
     3361 tggggggcac caccaacatc tggggcgggc gctgcgcctt gctcgacgac atcgatttcc
     3421 ggcgcaggcc gtgggtgccg cacagcggct ggcccatcac caatgcggag ctcacgcctt
     3481 actacgcggc cgt
//
LOCUS       DBNHOX010000374         2052 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_480268, whole genome shotgun sequence.
ACCESSION   DBNHOX010000374 DBNHOX010000000
VERSION     DBNHOX010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_480268"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="AC16UZ_08235"
     CDS             <1..973
                     /locus_tag="AC16UZ_08235"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6246709.1"
                     /translation="AVDRALADLSGEALRAYPQPTADRLRDQIAELHNLKRENIVVTN
                     GGDEGLRLAFTTFLEPGGTFGMADPSYSLYPVLAAIQDTRVLSIALDENWDLPRDFAH
                     QLNDAGAGLACLVNPHAPSGSLLDTSRISQLANDFNGVLLIDEAYVDFVDPALRYDAV
                     RLTEAFDNLLILRTFSKGYSLAGMRLGYLVGCASLIDPVIGKTRDSYNVDYVSQRVGE
                     AALSDPAYAEETWANVRAGRRTLREGLLEHGFRVGASQTNFLLTEVPAASPCSAKQLY
                     EALKTRGVLVRYFDTPSLRDTLRITVGTPEQNQTLLTWVGDILSRGE"
     gene            1068..>2052
                     /locus_tag="AC16UZ_08240"
     CDS             1068..>2052
                     /locus_tag="AC16UZ_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR6246710.1"
                     /translation="MTPKAGSYFPSQAASTLYAMAGDDCSLHFDLNTLTEKQLADHVS
                     NIELTAMLQVALLYVGKPVDHVALFDEFQRPPWRETLDLYRRAFTEMGVPPVPESNCH
                     YYPRDGFYEAVAADRVDADLTTLYLVSGSNAVVHRSDAALSVSQRVNSKMQLAADAAQ
                     WGIPVPETLVCRKRDLDGPEVSGFFDTHHNQIMLKLKGLAGARNVTSVAGVPECMDYV
                     AEYPNDMEIVLQRKLSADEFTEMTVDLIVRDDSIEIANVRRILFADGLWVGNLIGGGV
                     TVGPAHERALLRVGEYVRDLGYRSPEGFNCGIDYFVSADELLVTEINARWTG"
BASE COUNT          366 a          695 c          647 g          344 t
ORIGIN      
        1 ggccgtggac cgggcgctcg ccgatctcag cggcgaggcc ctgcgcgcct acccccagcc
       61 cacggccgat cgcctgcgcg atcagatcgc cgagctgcac aacctgaagc gcgagaacat
      121 cgtggtcacc aacggcggcg acgaaggctt gaggctggcc ttcaccacgt tcctggagcc
      181 gggcggcacc ttcggtatgg ccgatcccag ctactcgctc tatcccgttc tggcggccat
      241 tcaggacacc cgggtcctga gcatcgcgct ggacgagaac tgggacctgc cgagggactt
      301 cgcccaccag ctcaacgacg cgggggcggg actcgcctgc ctggtcaatc cccatgcccc
      361 atccggctcc ctgctcgaca cgtcgcggat cagccagctg gccaatgatt tcaacggcgt
      421 tctgctgatc gacgaggctt atgtcgattt cgtcgacccg gcccttcgct acgatgcggt
      481 gcgcctcacc gaggcgttcg acaatctgct catcctgcgc acgttcagca aggggtacag
      541 cctggccgga atgcgtctcg gctacctcgt cggctgcgcc tcgctgatcg accccgtcat
      601 cggcaaaacg cgcgacagct acaacgttga ctacgtgagc cagcgggtcg gcgaggccgc
      661 gctgtccgat ccggcctatg ccgaagagac ctgggcaaac gtacgcgccg gccgccgcac
      721 gcttcgcgag ggcctgctcg aacacggttt ccgcgttggc gcttcgcaga cgaattttct
      781 gctgaccgag gtgcccgcgg cgtctccgtg cagcgcaaag cagctctacg aggcgctcaa
      841 gacccgggga gttctggtgc ggtacttcga cacccccagc ttgcgggaca ccctgcgcat
      901 caccgtgggc acgcccgagc agaaccagac cctgctgacc tgggtggggg acatcctgag
      961 ccgcggagag tagggattag tatgcacggc ttcgcaatca ccgctgcgcg cccgctatcg
     1021 tccgcagctt cggccaacac atcgcaaccc cgacggagag tcagaacatg acaccaaagg
     1081 cgggttcgta tttccccagc caggcggcat ccacgctcta cgccatggcc ggcgacgact
     1141 gctctttgca cttcgacctg aacacgctga ccgagaagca gctggccgat cacgtatcga
     1201 acatagagct cacggccatg cttcaggtgg ctctgctcta cgtcggcaag cccgtggatc
     1261 acgtcgcctt gttcgacgag tttcagcgac cgccgtggcg cgagacgctg gacttgtatc
     1321 gtcgcgcgtt caccgagatg ggcgtgccgc ccgtgcccga gtccaactgt cactactatc
     1381 cacgggacgg gttctacgag gcggtggcgg cggaccgggt cgacgcggat ctgaccaccc
     1441 tctacctggt gagcggatcc aacgccgtgg tgcaccgcag cgacgccgcg ctttccgtca
     1501 gccaacgcgt gaactcgaag atgcagctgg cggcggacgc tgcgcaatgg ggcataccgg
     1561 tgcccgaaac cctggtctgc cggaagcgcg acctcgacgg ccccgaggta tccggctttt
     1621 tcgataccca ccacaaccag atcatgctga agctgaaggg tctggccggc gcgcgcaacg
     1681 tcaccagcgt ggctggcgtg ccggagtgca tggactacgt cgccgagtac ccgaacgaca
     1741 tggagatcgt gctgcaacgg aagctgagcg cggacgagtt caccgagatg accgtggacc
     1801 tgatcgtgcg ggacgacagc atagagatcg ccaacgtccg gcgcatcctg ttcgccgacg
     1861 ggctctgggt gggaaatctg atcggcggcg gcgtgacggt gggccccgcc cacgagcgcg
     1921 cgctccttcg cgtcggggag tacgtgagag acctcggcta ccgctccccg gaaggattca
     1981 actgcgggat cgactacttc gtcagtgccg atgagctcct ggtcaccgag atcaacgccc
     2041 gctggacggg cg
//
LOCUS       DBNHOX010000375        13270 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_994031, whole genome shotgun sequence.
ACCESSION   DBNHOX010000375 DBNHOX010000000
VERSION     DBNHOX010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13270)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13270)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13270
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_994031"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5616)
                     /locus_tag="AC16UZ_08245"
     CDS             complement(<1..5616)
                     /locus_tag="AC16UZ_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246711.1"
                     /translation="MKRKLALAVMSTLAMWVLAAAPAWAEKCSDYVDGIIDGNAVPAP
                     GQLTVDRDCYISNYDPLDTNITFIHNLGSFLVVFDNVRFRGNMSCDDVGDIHKVWFSD
                     SSSYPGRLPKCYRLWEPVDAVVKENPPGQTFVVIGVPFTYTLTMPVMPDGGPDVQDLY
                     DLRIVDDLTAGGADLRLVGFTSSIPGLVNTGTDTNLVLEHPRVPAGAQLVVELTVVAG
                     DTPRNAPGTTFTNFADWTFYKDLRSDGSPVFLPGDSSETPPLTIAGPELTLTKTGPAT
                     LNLGTSGEFTLDIQNVGNSDAWQVNVVDLLPDGASGGMCASSPTVTSARVFGPNGNSR
                     VQGKGWLVEGVDYFVSFTGSPRCQFTFETLSPAGVIAPDERLIITYEAELDPDTQDGA
                     VLTNVAGTPSWYDSDGSVSDRHLYTGSLTNGTVGVLDSEDAHTLTAELSGIFFEKTVA
                     NLSTGANPTATAAPGDRLRYTLRVQTTDSALNAFSIGDDLGALNANAVFEPGSLALVA
                     SSIPAGASTTNTNPNGGTNGTGLVDVRSLSLAAGSELTIQYDVTLADALLDGSVVSNQ
                     AILSGGSLQVDSDDPNVNGQSDPDVSGDEDPTRVLIEVAPPAALVKSTTQPTVSIGET
                     FTYRITVPSAPHGTPLFDVRITDNLAAVAADVDYVDVSRVSGPGSWTPVNTGSGNTLV
                     IEDTGAGIDIPAGEQVVIDITLRLTDSATNVAGLTFGNTAAYTYNQLNGDASSELDGL
                     SGTSADMTIVEPQLTVTKTGPGLMRVGPPATFTLDVQNTGGGRAHSVELTDALPNTAE
                     GGMCDAAPAAFTAQIFQADGATPVGPALAQGADFSVSFSGAPACTFNVDLLSAAATIG
                     PGERLIMTYQALLDADSLDDITLTNVAGATAWFSAATSQARGYAPELTDGSAGILDHE
                     DEHTILTFSPLLSFEKTVINVTTGESPAMTASPGDRLSYSLRVINLGPEPLDDFSVVD
                     ELDRLNRNPAFQPGTLQLTVVPDGATTGGTSASGGAAGTGYLEVSGLSLAGSGGTIDI
                     GFEVELAPVLANASLVRNQSQIIAQGFVVQDSDDPNKNDVADPVIVGDEDPTEVQVES
                     APAFDVDKTSTYLDGDPNVLMAGETLRYTITVQNVGDDNATNAVLRDQIPANTAYVAG
                     STTLNGASVADVGGTTPLAAGMTISSPADSAAGAMTAIASGSRSNVAVIEFDVVVDAS
                     AVDGTVLTNQAFLTATAGIIDQPSDDPDTPVADDPTRDVVGNLPLIYAEKSATLQTDG
                     GSRGVIDPGDVLRYTISIYNNGSADATDATLTDAVPVNTTYVANTVTLNGQPVGRPDG
                     GSFPLAGGLPVSSADVNPPVPGPGAGTITAGEVAVVQFDLRVDDDLPTGTLISNQAVV
                     STAEVVDQLTDGDGDPATGPEPTVVVVGPAQQLSITKQAAVVGGGAALAGSTLEYLVR
                     VRNVGPVPAIGVSLVDDLDYPTANQLQMVPGSATVNGQSVAATGSVLDIAFGDLDVDG
                     TITLRFQATLSAGLDAGTRVTNRAVATWNVNQQVEASVAVDVGGIVGVGVLNGSVWHD
                     ASYDDQLDSNERVLAGWTVELMRNGQVVHTTQTGTDGTYRITGLGPNAASSDSLSLTF
                     TAPGAGPGTALLGQASSASFTNGLQRIDDIVVQSGNNLMNLDLPIDPNGVVYDSVSRT
                     PVSGATLTLVDDVTRAPLPATCFDDTAQQNQVTRADGYYKFDLNFSDGACGSGASYTI
                     EVAAPGSAYTTGYSVLIPPTDLNAPTPFSVPGCPGSLDDAVPATGEHCEAGPSEFAPP
                     TSVQAQSSGTAYRVTGLVFDTRRLPGSAQIFNNHIPLDPILDGAVAISKTTPMVNVNR
                     GQLVPYTITVTNTFGVSLQDVAVIDRFPPG"
     gene            complement(5968..7419)
                     /locus_tag="AC16UZ_08250"
     CDS             complement(5968..7419)
                     /locus_tag="AC16UZ_08250"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HIR6246712.1"
                     /translation="MSDRKTPGNPATSKDQSADRPFEERSTGDALSERRRSITAQKEE
                     AQHRLDQLEAEATAARRELSELAARKEHFKLLEQAVAGLEALSESGAADLFWGADAPS
                     SMLRVEDARARIARFSSEVEQVEAKHQKFVSDVAMQQQAQEDLEYDLFQIEQQEESRR
                     QEWQVERDETVLPVRRIAMPWMRGFEEDERFKKTLAGSTAAALLLALIIPLIDLPMPE
                     KEELVEVPERFAKLIRQPQKPVTPPVQEQIAEVEPVKPKEVKPEKVVKPQPKREVAVQ
                     AKPAPKKNVNAVGILAFRKNLSDLRQNRPSANLGSKARVSNAGDTALDRPQRNMVTAQ
                     GTGSSGGINLADVSRDIGGGGVGLSEGVMLTQAESSIGGGGPDGRPGGTGGFAGRTDE
                     EIQIVFDRYKAALYRLYNRELRKDPTLRGQMVLRLTIEPDGSVSLCRVQSSDMDAPNL
                     AERVVSSVSRFDFGPKDVPPVTILYPIDFLPTA"
     gene            complement(7441..7953)
                     /locus_tag="AC16UZ_08255"
     CDS             complement(7441..7953)
                     /locus_tag="AC16UZ_08255"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR6246713.1"
                     /translation="MKSSRRIKRMDKTRKRSKFVTKMNLTSLMDVFTILVFFLLVNSG
                     AADPLESPKDVKLPESRAESKPKETVVIYISPETVVVQGQPVAMVADILAASGEDVVA
                     IRDRLAAIKGQIIGPNTQQVAGSQEVTIMADKSIPFSVVKRIMSTCTSEGYGNISLAV
                     VQKDAQVAAI"
     gene            complement(7950..8471)
                     /locus_tag="AC16UZ_08260"
     CDS             complement(7950..8471)
                     /locus_tag="AC16UZ_08260"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR6246714.1"
                     /translation="MARTHHYRRRVTEPTELDVTTFLNLMVVLVPFLLITAVFSRITI
                     VELDLPTSASSPSPNDPTFRVEVIVRDSGLEITNGRMVIAAIPKVEEEYDLQTLGGHM
                     LALKHDHPDIDEASVLLEPEIPYDYLIQVMDVVRSAEIQPEATEGNDGDGEVEQQPTQ
                     LALFTQIAIGDAP"
     gene            complement(8513..9175)
                     /locus_tag="AC16UZ_08265"
     CDS             complement(8513..9175)
                     /locus_tag="AC16UZ_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6246715.1"
                     /translation="MDFVYSIVEFFATGGAFMYPILLVFAVGAAIAIERFVTLTRVTT
                     KNQTAWSDIQPVLSQGDFERARKMTGQDQSPISRLLSMGLALQGAVRRREDVEIAMEE
                     GMMEIIPQLEKRTPYVALAASIATLLGLLGTIMGLIQAFTAVANANPAEKADLLSASI
                     SVAMNTTAFGLMVAIPLMVVHAFLSTKTNEIVDGMEMASVKVLTAISMRARRGEVTAK
                     AA"
     gene            complement(9313..9645)
                     /locus_tag="AC16UZ_08270"
     CDS             complement(9313..9645)
                     /locus_tag="AC16UZ_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246716.1"
                     /translation="MNRLLKLPLYFCLSALPAAASVAQEGDKAMSGMSILGNNDAPKA
                     LVIVPWKSSEIGDGIDVANLLDGRARAVDKVVFARELGYYELRVDAPEKSLSIREGAQ
                     MVNPVANQ"
     gene            complement(9651..10355)
                     /locus_tag="AC16UZ_08275"
     CDS             complement(9651..10355)
                     /locus_tag="AC16UZ_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6246717.1"
                     /translation="MYRPHSEPGPRIVLGGLILLAALLVGGCASTGPATRPALSKEAG
                     PTPTRERPARPARVKVEPNIGFTVTEVVALSSDVREMYHEALALLARNDLEAGIALLV
                     TVTERSPDATAPFIDLGIAHRRAGERDKAIASFTEALASTPDHPVAHNELGITYRELG
                     RFDEARRSYEQALTIYPGFHYARRNLAVLCDIYLADLACALEHYERYSQDIPDDREVE
                     IWVGDVRNRMPVTDEE"
     gene            complement(10336..>13270)
                     /locus_tag="AC16UZ_08280"
     CDS             complement(10336..>13270)
                     /locus_tag="AC16UZ_08280"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6246718.1"
                     /translation="VIGDGSGVTLAAPARLAMEDAVGASTEGDAAIADHSEPEAEFER
                     RATAPLALPSANWARAAEAGLPDGLSAAATAGPLEAIATYQKILDRYPYYERNDQVLY
                     QMARAYDELGEPDKAMEVMDWFINDYRYSHYADEVYFRRAEYFFVRKQYLEAEEAYSA
                     VVSIGSGSQFYELALYKLGWTFYKQDFYEEALHRFMALLDYKQSMGYDFDRVLAEQTS
                     DPDGAGDEEAAEDGDLDGQTGAAVAAVDVPGADATGEAEPDEDDDRRVADTFRVVSLS
                     FSNLGGPQVINEYYATYGNRSYEDRIYSNLGEFFLSKLRYNDAAKVYRSFTELHPFHR
                     IAPHFSMRVVEIYAKGDFGQLVVEAKKDFARRYGLNAEYWQHAEIEKSPDVLSYLKTN
                     LKDLASHYHALYQMEELEKERPGNYAEALTWYRELLASFPTDDDSAATNYQLADLQFE
                     NGDFTEAAVEYERTAYDYPAHEKASAAGYAAVYAHREHLKSIEAPPGSELRVAAVDST
                     MRFVDAFPAHDQAATVLGAATEDLYQMKRFEPAITAGRRLIESYPSAGEPLHLSAWTV
                     VAHASFDLAVYPDAEQAYAEVLALTPEADEGRQELFENLAASIYKQGEAASAMDDHRA
                     AADHFLRVRTAAPTSAFRPAAEYDAASALMKLESWAEASAVFRDFRAAHEDHELQPEV
                     TKQLAFVYRQAGELTRSAEEYERVAADALADGDETLRRESTLLAAELYEEAGSPKALA
                     AFQHYVETFPEPMELAVEVRFKIAGMHKADGDEAAYSGELRQIVAIDADAGAARTPRI
                     RYLAAQSALVLTEERFTHFTELRLSQPFEKSLAEKRKRMDAAMSAYEALVDYEVADVT
                     AAATYYMAEMYGHFGESLLSSERPKDLNEEELAEYELILEEEAFPFEESAIEVHEKNI
                     ELMVAGTFNTWIEQSLGQLSQLMPGRYAKSEISTGFVGPVEAYAYRTPVPIPAEETED
                     VSAAF"
BASE COUNT         2013 a         4200 c         4535 g         2522 t
ORIGIN      
        1 gccgggaggg aagcggtcga tcaccgccac gtcctgcagg gaaacgccga aggtgttggt
       61 caccgtgatg gtgtagggca cgagctggcc ccggttgacg ttcaccatgg gcgtggtctt
      121 gctgatcgcc acggcgccgt cgagtatcgg atcgagcggt atgtggttgt tgaagatctg
      181 cgccgagccg ggtaggcgcc tggtgtcgaa cacaagcccg gtcacccggt acgcggtgcc
      241 cgagctctgg gcctgcaccg aggtgggcgg tgcgaattcg gatggtccag cctcgcagtg
      301 ctcgcccgtg gcggggaccg cgtcgtcgag ggagcccggg caaccgggca cgctgaacgg
      361 cgtcggcgcg ttcaggtccg ttggcgggat gagcaccgag tagcccgtgg tgtacgcgct
      421 gccgggtgcg gccacttcga tggtgtagct cgcgccgctg ccgcaggcgc cgtccgagaa
      481 attcagatcg aatttgtagt agccgtccgc acgggtaacc tggttctgct gggccgtgtc
      541 gtcgaagcag gtggccggca gcggcgcgcg ggtgacgtcg tccaccagtg tcagggtcgc
      601 gccggaaacg ggggtgcgcg acacggagtc gtaaaccacc ccgttgggat cgatggggag
      661 gtccaggttc atcaggttgt tgccggactg cacgacgatg tcgtcgatgc gctgcaggcc
      721 gttggtgaag cttgccgagc tggcctgacc cagcagggcc gtgccgggcc ccgcgccggg
      781 cgcggtgaac gtcagggaca ggctgtcgct ggatgcggcg ttgggcccga ggcccgtgat
      841 ccggtacgtg ccgtcggtgc cggtctgggt agtgtgcacg acctgaccgt tgcgcatcag
      901 ctccacggtc catccggcca gcacgcgctc gttgctgtcg agctgatcgt cgtagctcgc
      961 atcgtgccag acgctaccgt tgaggacccc gacgccgacg atgccgccga cgtccacggc
     1021 cacgctggcc tcgacctgct gattcacgtt ccaggttgcc acggcgcggt tggtcacccg
     1081 ggtgccggcg tctagccctg cgctgagcgt cgcctggaat cgcagggtga tggtcccgtc
     1141 cacgtccagg tcgccgaagg cgatgtccag caccgagccg gtcgcggcca cggactgccc
     1201 gttgacggtt gccgagccgg gcaccatctg gagctggttc gccgtggggt agtccaggtc
     1261 gtcgacgagg ctgacgccga tcgcgggcac cgggcccacg ttgcgaacgc ggaccagata
     1321 ttcgagcgtg gaacccgcca gcgccgcgcc gcctcccacc acggccgcct gcttggtgat
     1381 gctgagctgc tgcgcgggac cgacgacgac cacggtgggc tcgggccccg tagcgggatc
     1441 accgtcgccg tccgtgagct gatcgacgac ctccgcggta gacacgacgg cctggttgga
     1501 gatgagcgtc cccgtgggga ggtcgtcgtc cacgcgaagg tcgaactgca ccacggcgac
     1561 ctcgccggcg gtaatcgttc cggcgccggg gcctggcacc ggtgggttca cgtcggctga
     1621 gctgacgggc aggccgccgg ccagcgggaa cgagccccca tcgggtcgac cgacgggctg
     1681 gccgttcagc gtgacggtgt tggccacgta ggtcgtgttg accggcaccg cgtcggtgag
     1741 cgttgcatcc gtcgcgtccg ccgagccgtt gttgtagatg ctgatggtgt agcggagcac
     1801 gtcaccggga tcgatgacgc cgcgagatcc gccgtcggtt tgcagcgtcg ccgatttctc
     1861 cgcgtagatg agcggcaggt tgccgaccac gtcccgggtg gggtcgtccg cgaccggtgt
     1921 gtccggatcg tcggagggct ggtcgatgat cccggcggtg gcggtaagga aggcctggtt
     1981 ggtgagcacg gtgccgtcga cggcggacgc gtccaccacc acgtcgaact cgatgacggc
     2041 tacgttgctc cggctgccgg atgcgatcgc cgtcatggcg cccgctgcgc tgtcggcagg
     2101 cgagctgatg gtcatgccgg cggccagggg cgtcgttccg cccacgtccg ccacggacgc
     2161 gccgttcagc gtggtgctgc ccgccacgta ggcggtgttt gcaggaatct gatcgcgcag
     2221 caccgcgttg gtcgcgttgt cgtcaccgac gttctgcacg gtgatggtgt agcgcagcgt
     2281 ttcgccggcc atcagcacgt tggggtcgcc gtccaggtag gtcgaggtct tgtcgacgtc
     2341 gaaggcgggt gcggactcca cctgcacctc ggtgggatcc tcgtccccga cgatcaccgg
     2401 gtctgccacg tcgttcttgt tcgggtcgtc gctgtcctgg acgacgaatc cctgagcgat
     2461 gatctgcgac tggttcctca ccaggctggc attggcgagc acgggcgcga gctcgacctc
     2521 gaagccgatg tcgatggtgc ccccggaccc ggcaaggctc agtccggaca cctccaggta
     2581 tcccgttccc gcagcgcccc cgctggcgct ggtgcccccg gtcgttgccc cgtcaggcac
     2641 gaccgtgagc tgcagggtgc ccggctgaaa cgccgggttc cggttcagcc ggtccagctc
     2701 gtcgacgacg ctgaaatcgt ccagcggttc cggacccagg ttgatcacgc gcaggctgta
     2761 gctcagccga tctccggggc tggccgtcat cgccgggctt tcccccgtgg tgacgttgat
     2821 gaccgtcttc tcgaaggaga gcaggggaga gaaggtaaga atcgtgtgct cgtcctcgtg
     2881 gtcgaggatc cccgccgagc cgtcggtcag ctcgggcgcg tagccgcggg cctggctggt
     2941 ggccgcgctg aaccaggccg tggcgcccgc cacgttggtc agggtgatgt cgtccaggct
     3001 gtcagcgtcc aggagcgcct ggtaggtcat gatcagccgc tcaccgggac cgatggtggc
     3061 tgcggcgctg agcaggtcca cgttgaacgt gcacgctggc gcgccggaga agctcacgct
     3121 gaaatccgcg ccctgggcga gagccgggcc caccggggtg gcgccgtctg cctgaaatat
     3181 ctgggcggtg aaggctgcgg gtgctgcgtc gcacatgccg ccctcggcgg tgttcggcag
     3241 ggcgtcggtg agctcgacgg aatgcgcacg gcctccgccg gtgttctgaa cgtccagggt
     3301 gaaggttgcg ggcggcccga cgcgcatcag ccccggtccg gtcttggtca ccgtcagctg
     3361 cggctcgacg atggtcatgt ccgcgctggt gcctgaaagc ccgtcgagct cgctgctcgc
     3421 atcgccgttg agctggttgt acgtgtaggc cgccgtgttg ccgaacgtca ggccggcgac
     3481 gttggtagcg ctgtccgtca atctgagggt aatgtcgatc accacctgct caccggccgg
     3541 gatgtcgatg ccggcgccgg tatcctcgat caccagggtg tttccggaac cggtgtttac
     3601 cggggtccag gatccggggc cggagacgcg ggaaacgtcc acgtagtcca cgtcggcagc
     3661 aacggctgcc aggttgtcgg tgatccggac gtcgaagagc ggcgtgccat ggggtgcgga
     3721 cggaacggtg atccggtacg tgaacgtctc gccgatgctc acggtgggct gggtcgtcga
     3781 tttcaccagg gcggcgggcg gcgcgacctc gatgagcacg cgggtgggat cctcgtcgcc
     3841 ggagacgtcg gggtccgact ggccgttcac gttgggatcg tcgctgtcga cctgaagtga
     3901 cccgccggaa agtatggcct ggttggacac cacggagccg tcgagcagcg cgtccgccag
     3961 ggtgacgtcg tactgaatgg tcagctcgct gccggcggcg aggctcaggc ttctgacgtc
     4021 gaccagcccg gtgccgttgg tcccgccgtt gggattggtg ttcgtggtgc ttgccccggc
     4081 aggaatcgag ctggccacca gagccagcga gccgggctcg aagacggcgt tggcgttcag
     4141 cgcgcccagg tcgtctccga tggagaacgc gttgagggcg ctgtccgtcg tctgcacgcg
     4201 cagggtgtag cgcagccgat cgccaggggc cgccgtggcc gtagggttcg cgccggtgct
     4261 gaggttggct accgtcttct cgaagaagat gccggaaagc tcggccgtca gcgtgtgggc
     4321 gtcctcgctg tcaagcacgc cgacggtccc gttggtcagg gagccggtgt agaggtgccg
     4381 gtctgaaacg ctgccgtcgg agtcgtacca ggagggggtg cccgccacgt tggtgagcac
     4441 cgcgccgtcc tgagtgtccg ggtcgagctc ggcttcgtag gtgatgatca ggcgctcgtc
     4501 cggggcgatg acgccggccg gcgagagggt ctcgaaggtg aactggcagc gcggggagcc
     4561 ggtgaacgag acgaagtagt cgacgccctc caccagccag cctttgccct ggacccgcga
     4621 gttgccgttg gggccgaata cccgcgcgct cgtcaccgtg ggggacgatg cgcacatgcc
     4681 gccgctggcg ccgtcgggca gcaggtcaac gacgttcacc tgccaggcgt cgctgttgcc
     4741 gacgttctgg atgtccaggg tgaactcgcc cgacgtgccc aggttgagcg tggcggggcc
     4801 cgtcttcgtc aacgtgagct cggggccggc gatggtcagc ggcggcgtct cgctcgagtc
     4861 gccgggcaga aagacggggc tgccgtcgct tcgcagatcc ttgtagaagg tccagtcagc
     4921 gaagttggtg aaggtggttc cgggcgcgtt gcgcggcgtg tcccccgcga ccacggtgag
     4981 ctcgaccacc agctgcgccc cggcgggcac gcgagggtgt tccagaacca ggttcgtatc
     5041 cgtgcccgtg ttcaccagac caggtatgga gctcgtgaag ccgaccagcc gcaggtcggc
     5101 gccgcccgcg gtgaggtcgt cgacgatccg aaggtcgtag agatcctgga cgtccgggcc
     5161 tccgtccggc atgacgggca tggtcagggt gtaggtgaac ggaacgccga tgacgacgaa
     5221 cgtctggcct ggcgggttct ctttgacgac cgcgtccacc ggttcccaca ggcgatagca
     5281 tttgggcagc cggccggggt aggaggacga gtcggagaac cagactttgt gaatgtcgcc
     5341 cacgtcgtcg cacgacatgt tgccgcggaa gcggacgttg tcgaacacga ccaggaagct
     5401 gcccaggttg tgaatgaagg tgatgttggt gtcgaggggg tcgtagttgg aaatgtagca
     5461 gtcacggtcc acggtgagct gtccgggggc cgggacggcg tttccgtcga ttatcccatc
     5521 gacgtaatca gagcacttct ccgcccacgc cggtgcagcg gcgagcaccc acatggcgag
     5581 ggtgctcatc accgcaagag cgagcttgcg cttcacgttt tgtttcgaac ttgccgacat
     5641 ctctccacga caccgcttgg tttggctgca ggtatctttc ccgcacgttg ccgtgcgctt
     5701 cgaccttgaa agcctcaccg tcgcgagcct cacgctggct tacgttcgtt gccgcaccgc
     5761 ttagcggtgc gctgtcccga aaccgcacta ttgccgacca gggctgcgcc cgtggtgatc
     5821 gccttcacac ccctgagcgt gtttccaagc ccgcgtgtcg aagccggagc agagtccacc
     5881 tgcgccatgc ggcattcgtc acaagtcata cgactgctgg attgccgcgc gctgctggcg
     5941 ggcccgcgct gagcgggcgc tcgccggtca cgcggtgggc aggaaatcga tggggtagag
     6001 aatggtgact ggcgggacat ccttgggccc gaagtcgaag cggctaacgc ttgagacgac
     6061 ccgctctgcc agattcggcg catccatgtc agaggactgc acgcggcaca gcgacaccga
     6121 gccgtcgggc tcgatagtca gtcgcaagac catctgaccg cgcagcgtcg ggtccttccg
     6181 gagctcgcgg ttgtataggc gataaagggc tgctttgtag cggtcgaaga cgatctggat
     6241 ttcctcatcc gtgcgtcctg cgaagccacc ggtgcctccc ggccggccgt cgggcccgcc
     6301 gccgccgatg gaactctccg cctgggtgag catcacgccc tcggacaggc cgaccccgcc
     6361 cccgccgatg tcccggctca cgtcggccag gttgatgccg ccgctggaac cggtgccctg
     6421 ggcggtgacc atgttgcgct ggggacgatc cagggcggtg tcgccggcgt tgctgacccg
     6481 ggccttggat ccgagattgg ccgaaggccg attctgacgc agatccgaga ggttcttccg
     6541 gaaagccaga atgcccacgg cgttcacgtt cttcttgggc gccggctttg cctgcaccgc
     6601 cacctctcgc ttgggctgag gcttgacgac cttctcgggc tttacctctt tgggcttgac
     6661 cggctcgacc tcggcgatct gctcctgaac cggaggcgtc acgggtttct gtggctgccg
     6721 gatcagcttg gcgaagcgct cggggacctc cacgagctct tccttctccg gcatgggcag
     6781 gtcgatgaga ggaatgatga gcgcgagcag cagcgccgct gcggtggacc cggccagggt
     6841 cttcttgaag cgctcgtctt cttcgaagcc gcgcatccac ggcatggcga tgcggcgcac
     6901 gggaaggacg gtttcgtcgc gctcgacctg ccactcctgg cgccggctct cttcctgctg
     6961 ctcgatctgg aagagatcgt attcgagatc ctcctgagcc tgttgctgca tcgcgacgtc
     7021 gctgacgaat ttctgatgct tggcttcgac ctgctcgacc tcggagctga atctggcgat
     7081 gcgcgctctg gcgtcctcga cgcgcagcat cgaggatggc gcgtcggccc cccagaagag
     7141 atcggcagca ccggattccg agagcgcttc caggcccgcc accgcctgct cgagaagctt
     7201 gaaatgctct ttgcgggcgg caagctcgct cagctcgcgc cgggcggccg tggcctcggc
     7261 ctccagctgg tcgagcctgt gctgcgcctc ttccttctgc gccgtgattg atcggcgcct
     7321 ttcgctgagc gcgtcgcccg tcgaccgttc ctcgaacggg cgatcagcgc tctgatcctt
     7381 cgaggtcgcc gggttgccgg gtgttttacg atcagacatg actaccttcg ctcatcccga
     7441 tcagatggcc gcgacctggg cgtccttctg caccacggcg agagagatgt tcccgtatcc
     7501 ctcgctggtg caggtggaca tgattcgttt caccacgctg aacggaatgg acttgtcggc
     7561 catgatcgtc acttcctggc tgcccgctac ctgctgcgtg ttcggaccga tgatctggcc
     7621 cttgatggcc gccagcctgt ccctgatagc aacgacgtcc tcgccggatg ctgcaagaat
     7681 gtcggcaacc atggccacgg gctgtccctg caccaccacg gtttccgggc ttatgtagat
     7741 gaccacggtc tccttgggct tggactcggc gcgcgactcc ggcagcttga cgtctttcgg
     7801 agactccagc gggtcggctg cgcccgagtt caccagcagg aagaaaacca ggatggtgaa
     7861 gacgtccatg agcgacgtca ggttcatctt cgttacgaac ttggagcgct ttcgcgtctt
     7921 gtccatacgc ttgatgcgtc gggagctctt catggcgcgt ctccaatggc gatctgggtg
     7981 aacagcgcga gctgggtcgg ctgctgctcg acttcgccgt caccgtcatt accttcggta
     8041 gcttccggct ggatctcggc gctgcgcacc acgtccatca cctgaatcag atagtcgtag
     8101 ggaatttccg gttccagcag aacggaggcc tcgtcgatgt cagggtgatc gtgcttcagc
     8161 gcgagcatgt gcccgccaag ggtctgaagg tcatactcct cttccacctt gggtatggcc
     8221 gcaatgacca tccgcccgtt ggtgatctcc agcccgctgt cacgcacgat gacctccacc
     8281 cggaaggtcg gatcgttcgg cgacggcgag ctggccgagg tgggcagatc cagctctacg
     8341 atggtgatcc tcgagaacac cgccgtgatg agcagaaacg gcaccagcac gaccatcagg
     8401 ttgagaaagg tggtcacgtc gagctccgta ggctccgtca cgcgccgccg atagtgatgg
     8461 gttctggcca tctgtccctt agcctccgtc taccccgttg gaatgcgtcg cttcaggctg
     8521 ctttcgcggt tacctcgccg cgacgcgcgc gcatcgagat ggcggtgagc actttgaccg
     8581 acgccatttc catgccgtcg acgatctcgt tcgtcttggt cgacaggaag gcgtgcacca
     8641 ccatcagggg gatggcgacc atcagcccga acgccgtcgt gttcatggcg accgagatgc
     8701 tggcagacag caggtcggct ttctccgccg ggttggcgtt ggccactgcc gtgaatgcct
     8761 ggatcaggcc catgatcgtg cccagcagtc cgagcagcgt tgcgatgctc gcggctaggg
     8821 cgacgtaggg cgtgcgcttt tccagctgag gaatgatttc catcatgccc tcttccatgg
     8881 cgatctcgac gtcctctcgg cgtcgcaccg caccctgaag agccagaccc atggacagca
     8941 gacgcgagat ggggctctgg tcctggcccg tcatcttgcg tgccctctcg aagtcaccct
     9001 gggacagcac cggctgaatg tcgctccatg ccgtctggtt cttggtggtc acccgggtca
     9061 gcgtcacgaa ccgctcgatg gcgatcgccg cgcctaccgc aaagaccagc aggatcgggt
     9121 acatgaaagc accgccagtg gcaaagaatt caacgattga gtacacgaaa tccattgcgt
     9181 aaacctctcc gcaaaacgcc ttgaatcaga cgcaacttaa agtgttgaga tgattgtgaa
     9241 cgtgatgggg cgcacccttc gccgttcggt tgttctgcca gttgatgagc ctcacgtttc
     9301 gacgccggcc gttcactggt tggcgaccgg gttcaccatt tgggcgcctt cccgaatgga
     9361 aagcgatttc tcgggcgcgt cgacgcgaag ctcgtagtag ccgagctctc ttgcgaacac
     9421 gactttgtcc actgctcttg cccggccgtc gagcaggttg gcaacgtcga tgccgtcgcc
     9481 tatctccgag ctcttccagg gcacgatgac cagcgccttg ggagcatcgt tgttgccgag
     9541 gatcgacatg cccgacatgg ctttgtcgcc ttcctgggcc acagaggccg ccgcgggaag
     9601 cgcgctcaga cagaaataca ggggcagttt caggagacgg ttcatcttgg tcactcctcg
     9661 tccgtcacgg gcatccggtt gcggacgtcg ccgacccaga tctcgacttc ccgatcgtcg
     9721 ggaatgtcct ggctgtatcg ctcgtagtgc tcgagagcgc aggcgaggtc ggccaggtag
     9781 atgtcgcaga gcacggcgag gttccgtcgc gcgtaatgga agccgggata gatggtcagg
     9841 gcctgctcgt agctgcgtcg cgcctcgtcg aagcggccga gctcacggta ggtgatgccc
     9901 agctcgttat gggccacagg atgatcgggt gtcgaggcca gggcctcggt gaagctcgcg
     9961 atcgccttgt cgcgctcgcc cgccctgcga tgggcgatgc cgagatcgat gaagggggcg
    10021 gttgcgtcgg gagacctttc cgtgacggtg acaagcagcg cgatgccggc ctccagatcg
    10081 ttccgcgcca gcagggccag cgcctcgtgg tacatctccc ggacgtcgct cgacagggcg
    10141 acgacctccg tcacggtgaa accgatgttg ggctccactt tcactcgtgc cggccgggcg
    10201 ggacgttccc gggtgggcgt cgggcctgcc tccttcgaca gggctggcct ggtggcgggc
    10261 cccgtgctgg cgcagccgcc gaccagcagc gcggccagca ggatcaggcc gcccagaacg
    10321 atgcgggggc ccggctcaga atgcggccga tacatcttct gtttcctctg ctggtatcgg
    10381 aacgggcgtc cggtaggcgt aggcttcgac gggccccacg aagccggtgc tgatttcgct
    10441 cttcgcgtag cgcccgggca tcagctgcga aagctggccg aggctctgct cgatccaggt
    10501 gttgaaagtc cccgcaacca tgagctcgat gttcttctcg tgcacctcga tggcgctctc
    10561 ttcgaacgga aacgcctctt cttccaggat gagctcgtac tccgcgagct cctcctcgtt
    10621 gaggtccttg ggccgctcgg agctcagcag cgactcgccg aagtggccgt acatctctgc
    10681 catgtagtag gttgccgcgg cggtcacgtc tgccacctcg tagtcgacca gcgcctcgta
    10741 ggcgctcatg gccgcgtcca tgcgcttgcg cttctccgcc aggctcttct cgaacggctg
    10801 gctcagccgc agctcggtga agtgggtgaa gcgctcctcg gtcagcacca gcgccgactg
    10861 cgcggcgaga taacgaatgc gtggcgtgcg tgcagcaccg gcgtccgcgt cgatggcgac
    10921 gatctggcgc agctcgcctg agtaggcggc ttcgtcgccg tcggccttgt gcatgccggc
    10981 gatcttgaag cggacctcca ccgcgagctc catgggctcg gggaaggtct cgacgtagtg
    11041 ctggaatgcc gcgagtgctt tcgggctgcc cgcctcctcg tagagctctg ccgccagcag
    11101 ggtggactcc cggcgcagcg tctcgtcgcc gtcggctaga gcatccgccg cgacccgctc
    11161 gtactcttcg gcggatcggg tcagctcgcc ggcctgccga tagacgaagg ccagctgctt
    11221 ggtcacctcg ggctgaagct cgtgatcttc gtgcgccgcg cggaagtccc tgaacacggc
    11281 gcttgcctcg gcccagctct ccagcttcat cagggcgctt gctgcgtcgt actctgcggc
    11341 gggtcggaac gccgaggtgg gcgctgcggt gcgcacgcgc aggaagtgat cggccgccgc
    11401 ccggtgatcg tccatggcgc tggctgcttc gccctgcttg tagatggagg cggccaggtt
    11461 ctcgaacagc tcctggcgcc cctcgtccgc ttcaggcgtc aaagccagca cctccgcgta
    11521 ggcctgctcg gcgtcgggat agacggccag gtcgaacgaa gcgtgcgcaa ccaccgtcca
    11581 cgcggaaaga tgcagcggtt cgccggccga cgggtaggac tcgatgagcc gcctgcccgc
    11641 ggtgatggcc ggctcgaagc gcttcatttg gtagaggtct tccgtcgcgg cgccgagcac
    11701 ggtggccgcc tggtcatggg cggggaaggc gtcgacgaag cgcatggtgc tgtccacggc
    11761 ggcgactcgc agctcgctgc ccggcggtgc ctcgatgctc ttcaggtgct ctcggtgggc
    11821 gtagacggca gcgtagccgg cggccgaagc cttctcgtga gccgggtagt cgtatgccgt
    11881 gcgctcatac tccacggcgg cttcggtaaa gtcgccgttt tcgaactgca gatcggcaag
    11941 ctggtagttg gtggccgcgg agtcgtcgtc ggtggggaag gatgccagca gctcccggta
    12001 ccaggtgagc gcctcggcgt agttcccggg ccgctccttt tcgagctctt ccatctggta
    12061 gagggcgtga tagtggctgg cgaggtcctt gaggttcgtc ttcaggtagc tcaggacgtc
    12121 gggcgacttc tcgatctccg cgtgctgcca gtactcggcg ttgaggccgt agcgacgggc
    12181 gaagtctttc ttggcttcca ctaccagctg gccgaaatcg cccttggcgt aaatctccac
    12241 cacgcgcatg ctgaaatggg gcgcgatcct gtggaaggga tgcagctcag tgaaggaacg
    12301 atagaccttg gccgcgtcgt tgtagcgcag cttgctcagg aagaactcgc cgaggttgct
    12361 gtagatccgg tcttcgtagc tgcggttgcc gtaggtggcg taatactcgt tgatgacttg
    12421 cggtccgccc aggttggaga aggacaggct caccacgcgg aaggtgtccg ccacgcggcg
    12481 gtcatcgtcc tcgtcgggct cggcttcccc ggtggcgtcg gcgcccggca cgtcgacggc
    12541 cgcgaccgcg gcgcccgtct gcccgtcgag atcgccatcc tcggcggcct cttcatcgcc
    12601 ggccccgtcg ggatccgatg tctgctcggc cagcacccgg tcgaagtcgt agcccatgga
    12661 ctgcttgtag tccaggagcg ccatgaaccg gtgcagggct tcctcgtaga agtcctgctt
    12721 gtagaacgtc cagcccagct tgtacaaagc cagctcgtag aactgcgagc cggaaccgat
    12781 ggagaccacg gccgagtagg cctcttcggc ctccagatac tgcttcctca cgaagaagta
    12841 ctcggcgcgg cggaagtaca cctcgtcagc gtagtgagag tagcggtagt cgttgatgaa
    12901 ccagtccatg acctccatgg ccttgtccgg ctcgccaagc tcgtcgtagg cccgcgccat
    12961 ctggtagaga acctgatcgt tgcgctcgta gtacgggtag cggtcgagga tcttctgata
    13021 ggtggcgatt gcttccagcg ggcctgcggt ggctgctgca gacagcccgt cgggcagacc
    13081 agcctcggcc gcacgcgccc agtttgcgga cggcagcgcc aacggcgcgg tcgcgcggcg
    13141 ctcgaattcc gcctccggct cggagtgatc ggcgatggcg gcatcacctt cggtgctggc
    13201 gcccaccgca tcctccatcg ccagacgcgc gggtgcggcg agcgtcacgc cgctgccgtc
    13261 gccgatgacg
//
LOCUS       DBNHOX010000376         6291 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_94093, whole genome shotgun sequence.
ACCESSION   DBNHOX010000376 DBNHOX010000000
VERSION     DBNHOX010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_94093"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="AC16UZ_08285"
     CDS             <1..714
                     /locus_tag="AC16UZ_08285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6246719.1"
                     /translation="YVIDGTKQWITNAVEGNVLAVLVKTDPDAVPAHRGMSLLIVEKS
                     AGYKATKLKKLGYRGVDTGEVQFDGVRVPAANLVGDGEGRGLQQILSGLALGRINVAA
                     RGVGVADASLQAALRYAQERQTFGVPIHDHQAIQIKLADMATRVEAARLLTEKAARIY
                     DTGQRCDLEAGMAKLFASEAALSNSLEAMRIHGAYGYSPDNDVERYYRDAPLMAIGEG
                     TNELQRIIIARQLIERNPI"
     gene            complement(740..925)
                     /locus_tag="AC16UZ_08290"
     CDS             complement(740..925)
                     /locus_tag="AC16UZ_08290"
                     /inference="COORDINATES: protein motif:HMM:NF016230.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6246720.1"
                     /translation="MYVCLCKAVTDKQIHEAVDAGVQTFTQLEESCGAGTGCGRCREV
                     AQELIDQRLADSAYYAA"
     gene            1132..1605
                     /gene="bfr"
                     /locus_tag="AC16UZ_08295"
     CDS             1132..1605
                     /gene="bfr"
                     /locus_tag="AC16UZ_08295"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HIR6246721.1"
                     /translation="MKPIDSDVIVHLNQVLRNELTAINQYFLHAKMFEDWGMHRIAAH
                     EKEESIDEMKHADALMERILFLEGLPNLQDLGKLYIGQDVKEILECDLKLELEAIPML
                     REAIAHCEHVGDYVSRQLFRDILSSEEEHVDWIETQLDLIAKTGVENYIQSQMQG"
     gene            1626..1919
                     /locus_tag="AC16UZ_08300"
     CDS             1626..1919
                     /locus_tag="AC16UZ_08300"
                     /inference="COORDINATES: protein motif:HMM:NF046888.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7009 family protein"
                     /protein_id="HIR6246722.1"
                     /translation="MSRAGLRLRLNGADLSALADGGTVTEPTPFPNGMFECSLTAVAD
                     GIGACLTERGVAVTIPTASLGDLEAGNGLRAELQTSGEALHVLIETDRRPEGR"
     gene            complement(1935..2504)
                     /gene="mobA"
                     /locus_tag="AC16UZ_08305"
     CDS             complement(1935..2504)
                     /gene="mobA"
                     /locus_tag="AC16UZ_08305"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HIR6246723.1"
                     /translation="MSLPPESLTALVLCGGAGRRMSGRDKPLLDLKGRSFVAWIAESV
                     KDRVSQVIISANRNLQAYLEVGEVLPDDGAGQGPLDGIATCARRCRTPFLFVCPGDAP
                     HLDAAVIDRLAEAMEKTDAPVVVASDGEQRQNLHLLMKSDESARILSYLESGRRSVSG
                     WLDEVGAVVVEMPELAGSFKDYDSSDDLA"
     gene            complement(2501..4348)
                     /locus_tag="AC16UZ_08310"
     CDS             complement(2501..4348)
                     /locus_tag="AC16UZ_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6246724.1"
                     /translation="MTTSNLLDGFTVGDFQVFPREEKIVGGDSSHHVEPKVMQVLVTL
                     ASNAGHTVSRDQILEEVWSDAVVGDEVLSRAISLLRGYFGDERTSPRYIRTVPRKGYE
                     LIAEVGAPPAKSTGAISRGRAYALVALVAALVVATAAVVRWWPATVDADLTLAVMPLA
                     ISGEASRLGYVADGLADHLITHLTRSPELHVVARRSSFLIRDTDANVRAIGEQLGARY
                     IVEGSLAERGANLLVTMYVVDTELGTNIWTTQLLGPANDLASLQVQAERAINEALRAR
                     LGVKLRTSRSAAGAISEDAYLKYHEARYQWSLRGELRIARAIALLQEAIDLAPDFAEA
                     HMALAQTLAVQPFYTDDPVAERFVKARASAARALELDASLAAEASALEGFLLWRERRW
                     QQAEIQLKKALRLDPDQVNAHYWYSWLLSALGKYDEAMREMQIAWRLNPVSAVLNDRL
                     AVAYLWVNDNAEAAARYQAAADLGYLESTQPESYILFLRRAGRFSEIAELLVRMGYSA
                     AWVEPFVRGLEHPDTRQEGAVAIEAASAAGDVPFELLFGIWVLFEDGDRAFANFDYGL
                     KTKYIEFLWADETEFLRDDARFADLIARLNLSDVIDLDEIRSANQDRAK"
     gene            4834..>6291
                     /locus_tag="AC16UZ_08315"
     CDS             4834..>6291
                     /locus_tag="AC16UZ_08315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR6246725.1"
                     /translation="MRKDKGNVTPLFGERRRPPESPRPQVVVLDSADFVDAGELGREL
                     DSGADLDRPRLVLLERNELAVLRPHLEADPNLEFILKPVDQDTLQARIFKLVATSEAF
                     RDRQQQLSRVQDHVDRLAYYDRITDLPNNEFFRRHVDVQIRHARRFTRQLAVLAVDLQ
                     SFERVRRRAGEAGTEALMSLSARRIIEAIRDYDVVGQSPNVLPFGDERTMARMEGDRF
                     LLLLPELRHIEEAASVAERVVAAVEVPIQIGGAAFITRPKVGISVYPTDGDDSTSLIR
                     CAQRALLSSSDLRGGRVHFHCEAMNDVIIDRLINEAALRRAFENGGFHLVYQPKVELS
                     TGRTTGVEALLRLVDPELGELPPEEIVPLAEQVGLISELTRWVLAEVCGQIRRWRQAG
                     LPRVPVSVNVSATDMLTPDFADFLTGLLLDNGVAPQALELEIPCRSFDGANDGMAAMV
                     AELRTVGVTIAVGDFGADGSALDCLDHLPVDALKID"
BASE COUNT         1175 a         1911 c         2029 g         1176 t
ORIGIN      
        1 tacgtgatcg acgggacgaa gcagtggatc accaacgcgg tggaaggcaa cgtcctggcc
       61 gtgctggtga aaacggatcc ggatgccgtt ccggctcaca gaggcatgag cctgctcatc
      121 gtcgaaaaat ccgcgggtta caaagcgacc aagctcaaga agctcggcta tcgcggcgtg
      181 gacaccggcg aggtgcagtt tgatggggtg cgggtgcccg cggccaacct ggtcggcgat
      241 ggtgagggcc ggggtctgca gcagatcctc tcgggactgg cgttgggccg gatcaacgtt
      301 gcggcccggg gggtgggggt ggccgatgct tcgcttcagg ctgcccttcg ctacgcccag
      361 gagcgccaga cgtttggcgt gccgatccac gatcatcagg ccattcagat caagctggcc
      421 gacatggcca cccgtgtgga ggctgcccgg ctgctcacgg agaaggctgc ccgaatttac
      481 gatacgggac agcgctgcga tctcgaggca ggcatggcga agctcttcgc atcggaggcc
      541 gcgctcagca actccctgga agcgatgcgc atccatggtg cttacgggta ctcgcccgac
      601 aacgatgtcg agcgctacta tcgagacgcg ccgctcatgg cgataggcga gggaaccaac
      661 gagctgcaga gaatcatcat cgcccgccag ctcatcgagc ggaatcccat ctgagaatgc
      721 ggtcgggccg gcggatcagt caggcagcgt agtacgccga gtcggcaagg cgctgatcga
      781 tgagttcctg tgccacctcg cggcagcggc cgcagccggt gccggccccg caggattcct
      841 cgagctgagt gaaggtctga acgccggcgt ccacggcttc gtggatttgc ttatcggtaa
      901 ctgctttaca gaggcagacg tacatctcaa ttgagcgatc ggatgaactg ctgttgattt
      961 aaatgcaaat cattctcggt tgcaatagtt tttggttctc gtttgtaacc ggatggtatt
     1021 ttcagatggg aatgcttttc aatataaatc agtcacttag agagaggcgg ccttgctaag
     1081 tccgactcgc tcccgcatag tccgtctttc gaaccggttc aggatcattc catgaagccc
     1141 atcgacagcg acgtcatcgt tcacctcaat caggtattga gaaacgagct cacggccatc
     1201 aatcagtact ttctccacgc aaagatgttc gaagactggg ggatgcatcg gatcgccgct
     1261 catgagaaag aggagtccat agacgagatg aagcatgccg atgcgctgat ggagcgcatc
     1321 ctgttcctgg aagggctgcc gaacctgcag gacctcggca agctctacat cggtcaggac
     1381 gtgaaggaga tcctcgagtg cgacctaaaa ctcgaactcg aagcgattcc catgctgcgt
     1441 gaggccatcg ctcactgcga gcacgttggc gactacgtca gccgacagct ctttcgcgac
     1501 atcctgtcat cggaggaaga gcacgtggac tggatcgaaa cccagctcga cctgatcgcc
     1561 aagaccggcg tcgagaatta catccagtcc cagatgcagg gctgagatca tcggtgaagc
     1621 tccggttgag cagagcgggc cttcgtcttc ggctcaacgg cgccgacctg tccgcactcg
     1681 ctgacggggg gacggtgacg gagccaacac cgtttcccaa cggcatgttc gagtgcagcc
     1741 tgacggcggt ggcggacggc atcggcgcgt gcctgaccga gcgcggcgtt gccgtgacga
     1801 taccgacggc ctcgcttggt gatctggaag ccgggaacgg cttgagggcg gagctgcaaa
     1861 cgtccggcga ggcgctccac gtgctcatcg agacagaccg ccggcccgag ggccgctgag
     1921 gcgctcctgc ccggtcaggc gaggtcgtcg ctcgaatcgt aatccttgaa agacccggcg
     1981 agttcgggca tctccacaac gacggcgccg acctcatcaa gccagcccga cacgctgcga
     2041 cggccagatt ccaggtagct tagaatgcgc gcgctctcat ccgacttcat gagcaggtgg
     2101 aggttctggc gctgctcgcc gtcgcttgcc acaaccacag gagcgtccgt cttctccatc
     2161 gcctccgcga gccgatcgat cacggcggca tccagatgcg gcgcatcgcc aggacacacg
     2221 aaaaggaacg gagttcggca tcgccgggcg caggtggcta tgccgtcgag agggccctga
     2281 cccgcgccgt cgtctgggag cacttccccg acttcgaggt aggcttgcag atttctgttg
     2341 gcgctgatga tcacctggct cacgcgatct ttgacgctct cggcaatcca cgccacgaag
     2401 gacctgccct tgaggtccag gagcggcttg tcgcgcccgc tcatccgacg ccccgcgccg
     2461 ccgcaaagaa cgagggccgt cagcgattcc gggggcaggc tcactttgcg cgatcttggt
     2521 tcgcgcttcg aatctcgtcg aggtcgatca cgtcggagag gttcaggcgg gcgatcaggt
     2581 cggcaaagcg ggcatcgtcg cgaagaaact cggtttcgtc cgcccagaga aactcgatgt
     2641 acttcgtctt tagtccgtag tcgaagtttg cgaatgcccg gtcgccatct tcgaacagca
     2701 cccagatgcc gaagagaagc tcgaagggta cgtcgcccgc cgcgctggcg gcctctatgg
     2761 ccaccgcgcc ttcctgtcgg gtatcgggat gttcgagccc gcgaacgaac ggctccaccc
     2821 acgctgccga gtatcccatt cgcacaagca gttcggcgat ttcgctgaag cggccggctc
     2881 gacgaagaaa caggatgtag ctctcaggct gggtgctctc caggtagccg agatcggcgg
     2941 cggcctgata tcgagcggct gcttccgcgt tgtcgttgac ccataggtac gcaacggcca
     3001 gtcggtcgtt tagcacggcc gatacgggat tcaaacgcca tgcgatctgc atttcgcgca
     3061 tcgcttcatc gtacttgccg agcgcggaca gcagccagga gtaccagtag tgggcattga
     3121 cctgatccgg gtcgaggcga agcgctttct tcagctgaat ctcggcttgc tgccagcgtc
     3181 tttcgcgcca gagcaggaac ccttccagcg ccgaggcttc tgccgccagg gaggcgtcca
     3241 gctcgagcgc ccttgctgcg ctcgcccggg ccttcacgaa ccgctcagcc accgggtcgt
     3301 cggtgtagaa aggctgtacc gccagggtct gggccagggc catgtgcgcc tcggcgaaat
     3361 cgggggccag gtcgattgcc tcctggagca gggcgattgc cctggctatt cgaagctcgc
     3421 cccgcaggct ccattggtag cgagcttcgt gatatttcag ataggcgtcc tcggagatcg
     3481 cgccggccgc cgaccggctc gttcgaagct tcacgccgag tcgggcccgc agagcctcgt
     3541 tgatggcccg ctcggcctgg acctgcagcg acgccagatc gttcgccggg ccgagcagct
     3601 gcgtggtcca gatgttggtg ccgagttcgg tatccaccac gtacatcgtc accagcagat
     3661 tggccccacg ctcggccaga gagccttcaa cgatgtagcg ggcgccgagc tgctcgccga
     3721 tggcgcggac gtttgcgtcg gtgtcccgga tcaggaagga cgatcgacgc gcgacgacgt
     3781 ggagctctgg ggatcgggtc aagtgagtga tcaggtggtc ggccagtccg tcagccacgt
     3841 accccaatcg actcgcttcc cctgagatgg ccagaggcat cacggccagg gtgaggtccg
     3901 cgtcaaccgt ggccggccac cagcgcacga cggctgcggt ggcgaccacc agtgcggcta
     3961 ccagggccac caacgcgtac gctcggcccc ggctgatcgc gcccgtgctc ttcgctgggg
     4021 gcgcgcccac ttcggcaatc agctcgtaac ctttgcgtgg gaccgtacgg atgtaacgcg
     4081 ggctcgttct ctcgtcgccg aagtagcccc gcagcaacga tatggcccgg gaaagcacct
     4141 cgtcgccgac cacggcgtcc gaccagactt cctctaagat ctggtcacgg gatacggtgt
     4201 ggccggcatt ggacgccagc gtcacgagca cctgcatgac tttcggctcg acatggtggc
     4261 tggaatcgcc gccgacgatt ttctcctccc ttggaaaaac ctggaagtcg ccgacggtaa
     4321 aaccgtcgag cagattgctt gttgtcattg ggcggcgtcc atggacgagg aggtcgatcg
     4381 acgactgccg ttggtgtgcg gggcgtgcgg gcagcgacgc cggctgcatc tgccaacgtt
     4441 gcccggcttt agggactcgt gctggttcat agcctgaaga taccgtcctc gggggctgcg
     4501 cgcatccgcg ttgttgtcac gccagcgccc ctgcgatcac cccgaggtta catgtcgctt
     4561 acaccgcgct gttatcaccc taacaggtaa ttgggtaggg tttacaaaac ggccgttatg
     4621 gcaacgaaat tgccaaatgt cgtcagtcta gctgcgctgc aggaaggctg atcgggccag
     4681 acactcggcg ggatgacctc gctgaagatt atagaaaagc cactcgatca tctgcgtgga
     4741 ttcgagttgg gggaaagcag aattcggtgc ttgttgccgc ggcgaacggg agggggtcgc
     4801 ggcaggcacc gggtctgcga tagtgagcac tggatgagaa aagataaagg caacgtgact
     4861 ccgctgttcg gggagaggcg gcgtccaccg gaatcgccca ggccccaggt ggtcgtgttg
     4921 gacagtgccg actttgtgga tgcgggcgaa ctcggccggg aactcgacag cggcgcggac
     4981 ctcgaccgcc cgcgtctggt attgctcgag cgaaacgaac tggccgttct gcgtccccat
     5041 ctggaagcgg acccgaatct ggaattcatc ctcaagccgg tagaccagga caccttgcag
     5101 gctaggatat tcaagctggt ggccacctcc gaagccttca gggaccgcca gcagcagttg
     5161 agccgggtgc aggatcacgt ggaccggctg gcctactacg acaggattac cgacctgccg
     5221 aacaacgagt ttttccgccg tcacgtggat gtgcagattc gccacgcgcg gcgttttacg
     5281 cgacagctgg cggtgcttgc ggtggatctg cagagcttcg agcgagtcag gcgacgcgcc
     5341 ggtgaagcgg gtaccgaggc cctgatgtcc ctgtctgccc gtcgcatcat cgaggctatc
     5401 cgggactacg acgttgtggg tcagtctccc aacgtgctcc cattcggaga cgagcgcacc
     5461 atggcgcgca tggaaggcga tcgctttctg ctgctgctgc cggaactgcg ccacatcgag
     5521 gaagccgcca gcgtcgcaga acgggtggtt gccgctgtgg aagtgcctat ccagatcggc
     5581 ggcgcggcgt tcatcacgcg accgaaggtc gggatcagcg tttacccgac ggacggcgac
     5641 gactccacat cgctcatccg ctgtgcccag cgcgcgttgc tgtcatcgag cgatttgcgc
     5701 ggtggccgag tccatttcca ctgcgaagcc atgaacgacg tgatcatcga ccggctgatc
     5761 aacgaggcgg cgctgcgtcg agcattcgaa aacggcggat tccacctggt gtaccagccg
     5821 aaggtggaac tctcgaccgg ccgcaccacc ggcgtggaag ctctgctgcg cctggtcgac
     5881 ccggagctgg gcgagctgcc tccggaggag atcgtcccgc ttgcggagca ggtgggcctg
     5941 atcagcgagc tcacccgatg ggtgcttgcc gaggtgtgcg ggcagattcg acgctggcga
     6001 caggccgggc tcccacgagt acccgtgtcc gtcaacgtgt ccgccacgga tatgctgacg
     6061 cccgatttcg ccgacttcct gaccggcctg ctgctcgaca acggcgtcgc gccccaggct
     6121 ctggagttgg agataccctg ccggagtttc gacggggcga atgacgggat ggcggctatg
     6181 gtcgccgagc tgagaactgt cggtgtgacc atcgccgtgg gcgactttgg cgccgacgga
     6241 tcggcgttgg attgcctgga tcaccttccg gtggacgctt tgaagataga c
//
LOCUS       DBNHOX010000377         2992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_864490, whole genome shotgun sequence.
ACCESSION   DBNHOX010000377 DBNHOX010000000
VERSION     DBNHOX010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_864490"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="AC16UZ_08320"
     CDS             complement(<1..855)
                     /locus_tag="AC16UZ_08320"
                     /inference="COORDINATES: protein motif:HMM:NF017039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutS N-terminal domain-containing protein"
                     /protein_id="HIR6246726.1"
                     /translation="MNTGSRTSHTPMMQQYLSIKARHPDDLLFYRMGDFYELFYEDAE
                     RAAELLDITLTARGQSAGKPIPMCGVPYHAADGYLARLVRQGVRVAICEQIGDPATSK
                     GPVAREVQRIVTPGTLTEEALQDARRDSILMAINPGPDEVYGLALLNLAAGRLQVAEV
                     RTRPLLAAEVARSDPSEILAVDELPEAVTGQRHLTRRDPLEFDTDLGRAKLTRHFATR
                     SLEGFDVPEDSPMVGAAAAVLEYAKSSQCRDLAFIDAMHRVAADDVVVLDPHSRRNLE
                     IDRRVDGSE"
     gene            989..2077
                     /gene="recA"
                     /locus_tag="AC16UZ_08325"
     CDS             989..2077
                     /gene="recA"
                     /locus_tag="AC16UZ_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR6246727.1"
                     /translation="MANEQSNRKDSQADSQQDPQRSRALSAALAQIERQFGKGSMMRL
                     GDRELVAIPAIPTGSLGLDIALGIGGLPRGRVVEIYGPESSGKTTLTLQVIAEAQKGG
                     GTCAFIDAEHALDPIYADALGVDTDNLLVSQPDTGEQALEICDMIVRSGAVDVVVIDS
                     VAALTPKAEIEGEMGDAHVGLQARLMSQALRKMTGNIKQSNTLVIFINQIRMKIGVMF
                     GSPETTTGGNALKFYSSVRLDIRRIGAVKEGEEVVGNETRVKVVKNKVAPPFRQVEFQ
                     ILYGEGINHLGEVLELGVQQGIVEKSGAWYAYKSDRIGQGRKNACDYLESNPEIAAEI
                     EAQIREQLLPVRERSEDLVIPEPAQETL"
     gene            2110..2598
                     /locus_tag="AC16UZ_08330"
     CDS             2110..2598
                     /locus_tag="AC16UZ_08330"
                     /inference="COORDINATES: protein motif:HMM:NF046475.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR6246728.1"
                     /translation="MLIEHKAVQEPDSAGDEQFQVAFARGLKLLATREHSAAELRRKL
                     RVKGFSEALAGDAVAALQDQGLQSDRRFAEVFVRSRLSKGYGPVRIRRDLYQRGIGDD
                     VIDEELTFPAERWVELATAVRAKRFPESEPTEHGVWNVQARFLSRKGFPADIIYRVLG
                     QV"
     gene            2716..>2992
                     /locus_tag="AC16UZ_08335"
     CDS             2716..>2992
                     /locus_tag="AC16UZ_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HIR6246729.1"
                     /translation="MNTAEIRSAFLSFFAEREHRVVPSSSLVPANDPTLLFTNAGMNQ
                     FKDALLGREDLGYRRAATAQRCVRAGGKHNDLENVGYTARHHTFFEML"
BASE COUNT          551 a          908 c          983 g          550 t
ORIGIN      
        1 ctcgctgccg tccacgcgcc gatcgatttc cagattgcgc cgtgagtgcg gatccagcac
       61 gaccacatcg tccgcagcca cgcgatgcat ggcgtcgatg aacgccagat cgcgacactg
      121 cgaggacttt gcgtactcga gcacggcggc cgcggcgccc accatgggcg agtcctcggg
      181 gacgtcgaag ccctccaggg agcgcgtcgc gaagtgtctg gtcagcttgg cccggccgag
      241 atcggtgtcg aactccagcg gatcgcgccg cgtcaggtgg cgctgtccgg tgaccgcttc
      301 gggcaactcg tcgacggcca agatctcgct cggatcgctg cgggccacct cggctgccag
      361 cagcgggcgg gtccgcacct ctgcgacctg cagcctgccg gccgcaaggt tcaacagcgc
      421 caggccgtag acctcgtccg ggcccggatt gatggccatg aggatgctgt cgcgtcgggc
      481 gtcctggaga gcctcctcgg tgagggtgcc gggggtgact attctctgga cctctctggc
      541 caccggtccc ttgctggtgg ccgggtcgcc gatctgctcg cagatggcca cccgcacgcc
      601 ctggcgcacc aaccgggcga ggtacccgtc agccgcgtgg tagggcacgc cacacatggg
      661 tatcggcttg cccgccgact ggccacgggc agtgagcgtg atgtccagca gctcggccgc
      721 gcgctcggca tcctcgtaga aaagctcgta gaaatcgccc atgcggtaga acagcaggtc
      781 gtccggatgc cgggccttga tgctcaggta ctgctgcatc atcggcgtgt ggctggttct
      841 ggatcccgtg ttcatgaacc tattattctc tgtggctccg agatgtctca agagcatgtg
      901 cgagaacgta tagaagggcc cttcgagcgg ttgccatcaa tactgattat atatacagta
      961 ccggccgtta accccgcagg agagattcat ggccaacgag cagtccaacc gcaaagattc
     1021 gcaggcggat tcccagcagg atccgcagag atcccgggcg ctgtccgcgg cgctggcgca
     1081 gatcgagcgc cagttcggca aaggctcgat gatgcggctt ggagatcggg agctggtcgc
     1141 cataccggcg atcccaacgg gctcgctggg gctagacatt gctctgggta tcggcggtct
     1201 gccaaggggc cgggtggtcg agatctacgg tccggagtcg tcaggtaaga cgacgctcac
     1261 cctccaggtc attgccgagg ctcaaaaggg cggcggcacc tgcgctttca tcgacgcgga
     1321 gcacgccctc gaccccatct acgccgatgc gctcggtgtc gatacggaca atctgctggt
     1381 atcccagccg gataccggcg agcaggctct ggaaatctgc gacatgatcg tccgttccgg
     1441 agccgtggac gtggtggtca tcgactccgt ggcggcactg acccccaagg cggagatcga
     1501 aggggaaatg ggggatgccc acgtcggcct gcaggctcgt ctgatgagcc aggcgcttcg
     1561 aaagatgacg ggcaacatca agcagtcgaa caccctggtc atcttcatca atcagattcg
     1621 tatgaagatc ggggtcatgt tcggttctcc ggaaaccacc accggcggga acgcgctgaa
     1681 attttactcg tcggtgcggc tggacatccg ccggatcggc gccgtgaagg aaggcgaaga
     1741 ggtggtcggc aacgagactc gggtcaaagt ggtcaaaaac aaggtggctc cgcccttcag
     1801 gcaggtcgag ttccagatcc tttacgggga gggcatcaat cacctcggtg aggtcctgga
     1861 gttgggtgtg cagcagggca tcgtcgagaa atcgggtgcc tggtatgcct acaagtccga
     1921 tcggatcggg cagggacgga agaacgcctg cgactacctc gagtccaatc ctgaaatcgc
     1981 ggccgagatc gaagcccaga ttcgcgagca gctcctgccc gttagagagc gttccgagga
     2041 tctcgtcatt cccgagccgg cccaggaaac gctgtagacg aatcaggccc cggggagcgc
     2101 ggtggtcgca tgttgattga gcacaaggcg gttcaggagc cggacagcgc gggcgatgag
     2161 cagttccagg tggcgtttgc ccgaggcctc aagctgctcg ccacccggga gcattcggcg
     2221 gccgagcttc gacgcaagct tcgcgtcaaa ggattttctg aggcgttggc cggagacgcc
     2281 gtcgctgcgc tgcaggacca ggggctgcaa tccgaccggc gcttcgcgga ggtgtttgtt
     2341 cgcagtcgtc tcagcaaggg gtacgggccc gtccgaattc gtcgggatct atatcaacgc
     2401 ggcatcggcg atgacgtgat tgacgaggag ctgaccttcc cggcggaacg ctgggtcgaa
     2461 ctcgccactg cggttcgggc caaacggttt ccggaaagcg agcccacaga gcacggcgtc
     2521 tggaatgtgc aggcgcgttt tctgtcccgc aaggggtttc cggccgacat catttaccgg
     2581 gtgctggggc aggtctaggc gcccaatctc gccgccgcgg aggccctgtc cgtcaccccg
     2641 tggcagacca ggcggccatg tcaattggac gcggtttccg tatactgcgc gcctcctaat
     2701 tggttgattc caaagatgaa caccgctgaa atccgttcag cattcctgtc gttcttcgcc
     2761 gagcgggagc accgcgtcgt gccgtcgagt tccctggtgc cggccaacga tccgaccttg
     2821 cttttcacca acgccggaat gaaccagttc aaggacgcgc tgctcggtcg cgaggatcta
     2881 ggctatcgtc gcgcggcgac ggctcagcgt tgcgtcaggg cgggtggtaa gcacaacgat
     2941 ctggaaaacg tcggatacac ggctcgccac cacacgtttt tcgagatgct cg
//
LOCUS       DBNHOX010000378         4231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1508590, whole genome shotgun sequence.
ACCESSION   DBNHOX010000378 DBNHOX010000000
VERSION     DBNHOX010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1508590"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="AC16UZ_08340"
     CDS             complement(<1..259)
                     /locus_tag="AC16UZ_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HIR6246730.1"
                     /translation="MLSKDLEVTLNEAFRAAKAKRHEFMTVEHLLLALLDNPTVIELF
                     DSCAVDRERLRRDLDEFLDETSPLLAPNDTRDIQPTVGFQRV"
     gene            complement(293..631)
                     /gene="clpS"
                     /locus_tag="AC16UZ_08345"
     CDS             complement(293..631)
                     /gene="clpS"
                     /locus_tag="AC16UZ_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017641761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HIR6246731.1"
                     /translation="MQAQSGEGDADDSSDSGLATVTAKPELKRPPMYKVVLLNDDYTP
                     MEFVVHVLEIFFAMDREKATQIMLTVHTQGAAVVGIYPRDIAETKSEQVNQYAQENQH
                     PLISTIEMTD"
     gene            922..1242
                     /gene="cspD"
                     /locus_tag="AC16UZ_08350"
     CDS             922..1242
                     /gene="cspD"
                     /locus_tag="AC16UZ_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HIR6246732.1"
                     /translation="MAMGKVKWFNNAKGYGFILPDGGGDDLFAHYSSIQMDGYKTLKA
                     GQDVEFDILEGPKGLHAINIRAEAIESEDSGEDAPVDESAAADPGPAEASPPARETSR
                     EATS"
     gene            complement(1232..2488)
                     /gene="icd"
                     /locus_tag="AC16UZ_08355"
     CDS             complement(1232..2488)
                     /gene="icd"
                     /locus_tag="AC16UZ_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR6246733.1"
                     /translation="MTYQHIEVPADGEKITVNADDSLNIPDNPIIPFIEGDGIGVDIT
                     PVMKDVVDAAVAKAYGGSRRIHWMEIYSGEKSLEVYGEDEWLPAETLDAMREFIIGIK
                     GPMTTPVGGGIRSLNVALRQELDLYVCQRPVRWFQGVPSPVVAPEKTDMVIFRENTED
                     IYAGVEWEADSEGADKLIAFLRDEMGVNKIRFPQHCGIGIKPVSVEGSKRLIRKALQY
                     VIEEDRDSLTFVHKGNIMKFTEGAFKDWGYELAAEEFGATPLDGGPWHELTNPRTGKR
                     VIIKDVIADAMLQQILTRPDEYDVIATMNLNGDYLSDALAAQVGGIGIAPGANIGDTI
                     ALFEATHGTAPKYAGQDKVNPGSLVLSAEMMLRHMGWREAADLIIQGVEGAIGERTVT
                     YDFERLMSGATLLKCSEFGQAIVAKM"
     gene            complement(2614..3093)
                     /locus_tag="AC16UZ_08360"
     CDS             complement(2614..3093)
                     /locus_tag="AC16UZ_08360"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003316924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030366 - molybdopterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HIR6246734.1"
                     /translation="MHSEVRIQEADFSVAAQWEALRGRASGEVGAVASFVGLVRDRDP
                     DAPIQGLYLEHYPGMTEASIGKILDETASRWPILDIVVIHRVGGLEPGDQIVFVQVAA
                     AHRPAAFAACEFVMDYLKTDAVLWKRERLMEGERWIEATGDDRRRVARWKADPVGDS"
     gene            complement(3096..3353)
                     /locus_tag="AC16UZ_08365"
     CDS             complement(3096..3353)
                     /locus_tag="AC16UZ_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR6246735.1"
                     /translation="MIPVRVLFFASLREAVGREEVFLELPDSARIDDLLASLDDILSA
                     EAMAALRAENVRLAVNQEMLTAPGRLRPNDEVAFLPPVTGG"
     gene            complement(3350..3826)
                     /gene="moaC"
                     /locus_tag="AC16UZ_08370"
     CDS             complement(3350..3826)
                     /gene="moaC"
                     /locus_tag="AC16UZ_08370"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019821998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR6246736.1"
                     /translation="MSKLTHLNAAGEASMVDVSGKSETLRRAVARGIVRMAPETLREI
                     LQSGVPKGDVIAVARVAGIQAAKRCADLIPLCHPLPLTKVALDFSPAGDDGLEVTAEC
                     VVTGQTGVEMEALTAVSVAALTVYDMCKAIDRGMVIESVCLVSKEGGRSGTWERTP"
     gene            3964..>4231
                     /gene="mnmA"
                     /locus_tag="AC16UZ_08375"
     CDS             3964..>4231
                     /gene="mnmA"
                     /locus_tag="AC16UZ_08375"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853241.1"
                     /note="catalyzes a sulfuration reaction to synthesize
                     2-thiouridine at the U34 position of tRNAs; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR6246737.1"
                     /translation="MSGGVDSSVAALLLLQEGYAVEGLFMKNWEEDDGTEYCTAKEDL
                     ADAQRVAERLGITLHLANFAAEYWDNVFEHFLAEYDAGRTPNPDV"
BASE COUNT          773 a         1348 c         1314 g          796 t
ORIGIN      
        1 gcacgcgctg gaagccgacc gtcggctgga tgtcgcgggt gtcgttgggt gccagcagcg
       61 ggctggtctc gtccaggaac tcgtcgaggt cgcggcggag ccgctcgcgg tcgacggcgc
      121 aggagtcgaa cagctcgatc acggtggggt tgtcgagcag cgccagcagc aggtgctcga
      181 cggtcatgaa ctcgtgacgc ttggctttgg cagcgcggaa cgcttcgttc agggtaactt
      241 ctaaatcttt actcagcatg aggtgtgtct ggcttcgtcc tgtgttcggt gatcaatcgg
      301 tcatctctat cgtcgagatc agcggatgct gattctcctg agcatattga ttcacttgct
      361 cggatttagt ttcagcaatg tcgcggggat agatccccac caccgccgcg ccctgggtgt
      421 gcaccgtgag catgatctgc gtcgctttct cgcgatccat ggcaaaaaag atctccagca
      481 cgtgcacgac gaactccatg ggcgtgtaat cgtcgttcag cagcaccacc ttgtacatcg
      541 gcgggcgctt gagctccggc ttggcggtga ccgtcgcgag ccccgaatcg gagctgtcgt
      601 ccgcgtcacc ctcgccggat tgcgcttgca acgggtcgct cttcagaacc ctgggcggta
      661 tctgggaacg atttgttggc tccattgctc ctcgttcatg tgggcggcag cacgatgata
      721 caagcatccc cgggccttga ctaagcccga aaatccatgg aaagtcgggc tcatagggga
      781 gctgcaactt gtgaccgttc gattatacgc ggcgggacgg gtacaaagat gcgcaacact
      841 gcgcgcttcg cgcagtttgg gcggcagggc cgaggtacgc acaaggatgg gcgttgtgtt
      901 cgtggctgct agggggtctc catggcgatg ggaaaagtaa agtggtttaa caatgccaag
      961 gggtacggat ttatcctgcc cgacggcggt ggcgacgatc tgtttgcgca ctactcttcc
     1021 attcagatgg acggctacaa gactctcaag gccggccagg acgtggagtt cgacattctg
     1081 gagggcccta aaggcctgca cgccatcaac atacgcgcgg aggcgattga atccgaggat
     1141 tcaggcgagg acgcccccgt cgacgagagc gcggcagctg atcccggacc ggcagaggcc
     1201 tctccaccgg cccgagaaac ctctcgcgag gctacatctt agcaacgatg gcctgaccga
     1261 attccgagca cttcaacaac gtcgcgccgc tcatcaggcg ctcgaaatcg taggtcaccg
     1321 tccgctcgcc aatggcgcct tccacaccct gaatgatcag gtcggcggcc tcgcgccacc
     1381 ccatgtggcg gagcatcatc tctgccgaga gcaccagcga gccgggattc accttgtcct
     1441 gcccggcgta cttcggcgcg gtgccgtggg tcgcttcgaa cagcgcgatg gtgtcgccga
     1501 tgttggcacc aggcgcgata ccgataccgc cgacctgagc ggccagggca tcggacaggt
     1561 agtcgccgtt cagattcatg gtggcaatca cgtcgtactc gtctggccgg gtgaggatct
     1621 gctgcagcat ggcatcggcg atcacgtctt tgatgatgac ccgcttgccc gtgcgggggt
     1681 tggtcagctc atgccagggg ccgccgtcca gcggggtcgc gccgaactcc tccgcggcta
     1741 gctcgtagcc ccaatccttg aaggcgccct cggtgaactt catgatgttg cccttgtgca
     1801 cgaaggtcag cgagtcccgg tcctcctcga tgacgtactg aagggctttg cggatcaggc
     1861 gcttgctgcc ctcgacggaa acgggcttga tgcctatgcc gcagtgctga ggaaagcgga
     1921 tcttgttgac gcccatctcg tcgcgcagga aagcgatgag cttatcggcc ccttccgaat
     1981 ccgcctccca ttccacgccc gcgtagatgt cctcggtgtt ttctctgaag atgaccatgt
     2041 ccgtcttctc tggggccacc accgggctgg ggacaccctg gaaccagcgc accggacgct
     2101 gacagacata caggtccagc tcctgtcgca gagcgacgtt caaagaccgg atgccgccgc
     2161 ccaccggcgt ggtcatgggg cccttgatgc cgatgatgaa ctcccgcatg gcgtcgaggg
     2221 tctccgccgg cagccactcg tcttccccgt agacctccag ggatttctcg ccggagtaga
     2281 tctccatcca gtggatcctg cggctgccgc cgtaggcctt ggccaccgcg gcgtccacca
     2341 cgtccttcat gaccggggtg atgtcgaccc cgatgccgtc cccctcaatg aaagggatga
     2401 tcgggttgtc ggggatgttc agtgaatcat ctgcgttgac ggtgatcttc tcgccgtcag
     2461 cgggcacttc gatgtgctga taggtcatct aaagctccgt ttggaaatcc gtttgggtaa
     2521 cagccttgac ccgtcgcgat aggtcgcgat ggaaaggcgc accggaaaaa ggcgcgagat
     2581 tatagccctt ccgacgaggc tgtgcagtgg gtatcaggaa tcgccgaccg ggtcggcctt
     2641 ccatcgggcg acacggcgac ggtcgtcccc ggtcgcctcg atccagcgct cgccttccat
     2701 cagccgctcc cgcttccaca gcacggcgtc ggttttcaga tagtccatga cgaactcgca
     2761 ggcagcgaag gctgccgggc ggtgcgcggc cgccacctgc acgaacacga tctggtcgcc
     2821 gggttcgagg ccacccacgc ggtgaatgac cacgatgtcc aggatgggcc agcgtgatgc
     2881 ggtctcgtcg agaatcttac cgatgctcgc ttccgtcatg ccgggataat gctcgagata
     2941 cagaccctgg atgggcgcat caggatcgcg gtccctcacg agcccgacaa agctcgctac
     3001 cgcgccaacc tcgcccgacg cgcgcccgcg cagcgcctcc cactgtgccg ccacgctgaa
     3061 atccgcttcc tgaattcgaa cctcggaatg cacggtcagc cccccgttac cggcgggagg
     3121 aaggcgacct catcgttggg acgaagccgc ccaggggccg tgagcatctc ctggttgacg
     3181 gccaggcgaa cgttttccgc ccgcagcgct gccatcgcct cggcgctgag aatgtcatcc
     3241 agcgaggcga gcaggtcgtc gatccgcgcc gaatccggca gctcgaggaa gacctcctcg
     3301 cgaccgacgg cttccctgag agaagcgaaa aacagaacac ggacgggaat cacggcgtgc
     3361 gttcccaggt gccgctgcgg ccgccttctt tgctcaccag gcacacggac tcgatgacca
     3421 tgccccggtc gatggctttg cacatgtcat agaccgtcag cgcagccacg ctgacggcgg
     3481 tcagggcttc catctcgacc ccggtctggc ccgtgaccac gcactcggcg gtaacctcga
     3541 gaccgtcgtc gccggccggg ctgaaatcca gagccacctt ggtgagcggc aacggatggc
     3601 aaagcggaat gagatccgcg caccgtttgg cggcctgaat gccggctacg cgggcgacgg
     3661 caatgacgtc acccttggga acccccgact gcaggatctc gcgaagggtc tccggcgcca
     3721 tccgcacgat cccccgggcc acggcacgcc gcagcgtctc ggacttgccg gagacgtcga
     3781 ccatgctcgc ctcgcccgcg gcattgagat gggtaagctt gctcatggtc gcattatagc
     3841 ccccaacggg ctgtcgggcg ggcaagaacc cctgcttggg ggtagaatgg ccatccttca
     3901 cacagcagtg ctgcacgcac ccctgaagtg gcaactccca ggcaaaccaa agtcatcgtc
     3961 ggcatgtcgg gcggtgtcga ctcctccgtc gccgccctcc tgctcctgca ggaggggtac
     4021 gccgtggagg gcctgttcat gaagaactgg gaagaggacg acggcaccga gtactgcacg
     4081 gccaaagagg atctggccga cgcccaacgg gtcgccgaac gcctgggcat aactctgcac
     4141 ctggcgaact tcgctgccga gtactgggac aacgtcttcg agcactttct cgccgagtac
     4201 gacgcgggcc gcacgccgaa tcccgacgtg c
//
LOCUS       DBNHOX010000379         1780 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_930267, whole genome shotgun sequence.
ACCESSION   DBNHOX010000379 DBNHOX010000000
VERSION     DBNHOX010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_930267"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..692)
                     /locus_tag="AC16UZ_08380"
     CDS             complement(3..692)
                     /locus_tag="AC16UZ_08380"
                     /inference="COORDINATES: protein motif:HMM:NF041238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylhydroperoxidase-related (seleno)protein"
                     /protein_id="HIR6246738.1"
                     /translation="MYPDDLPLSEALRNTHQKTLAKIVSPGPHWSGSERRAMAESARA
                     AEHCGLCKTRKTALSPLGVEGHHDGATTLAPTLLDAIHRIRTDPGRLTKRWFEAVAAE
                     IGEGPYVEMLSVVNSLVIVDTLHRCLGQSPPALPDPVAGGPTGEPAGDVVEAGAWVPI
                     ARAPRDMADTGLPVVPNIVRAMGLVPKAVELFFGTFRPHYALKDVNLSLSQAQAEYVA
                     SRVSAMNECFY"
     gene            complement(814..1581)
                     /locus_tag="AC16UZ_08385"
     CDS             complement(814..1581)
                     /locus_tag="AC16UZ_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR6246739.1"
                     /translation="MSARYKPPRRRNLYEPGSAKPFKISRSKIDRFVECPRCFYIDRR
                     LGTDRPPGFPFNINSAVDTLLKTEFDVHRSEGSQHPLQAAYGIDAIPAAHANIDVWRE
                     NFKGVQHLHEPTNLIITGAIDDLWIDGDGSYIVVDYKATAKKEPVTSMEAAWMDGYKR
                     QMEIYQWLLRRNGLDVSDTGYFVYCTGRPDAEAFDAKIDFDVHLIPYQGNDDWVEPTI
                     VALHACLNQPEIPQASWRCDYCAFVGAVNDVTGRSET"
     gene            complement(1591..>1780)
                     /locus_tag="AC16UZ_08390"
     CDS             complement(1591..>1780)
                     /locus_tag="AC16UZ_08390"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HIR6246740.1"
                     /translation="FLTAFSDSDPVTKGQEQRFIDSVPGAQGQKHTIIRGGGHFLQDD
                     VGEELAAVVIEFMQDNPL"
BASE COUNT          314 a          561 c          572 g          333 t
ORIGIN      
        1 gttcagtaga agcactcgtt catggccgac actcgggacg ccacgtactc cgcctgtgcc
       61 tgagagagag aaagattgac gtccttcaga gcgtaatgcg gccggaacgt gccaaagaag
      121 agctctaccg ctttgggcac cagccccatg gctcggacga tgttcggcac cacgggcagg
      181 cccgtatccg ccatgtccct cggcgcgcgg gcgatcggta cccaggcgcc ggcttccaca
      241 acgtccccgg cgggctcccc cgtgggccca ccggccaccg ggtccggcaa agcgggcggt
      301 gattgaccaa gacaccggtg cagtgtgtcg acgatcacca ggctattgac cacgctgagc
      361 atctccacgt aggggccctc gccgatctcc gccgcgaccg cctcgaacca gcgtttggtc
      421 agccgcccgg gatccgtgcg gatacgatgg atggcatcca ggagcgtcgg cgcgagagtc
      481 gtggcgccat cgtggtggcc ttcgaccccc aaaggagaga gggccgtctt gcgcgtcttg
      541 cacaacccac agtgttcggc ggctctggcg gattccgcca tggcacgccg ttcactgccc
      601 gaccagtgag ggccggggct gacgatcttg gcgagcgtct tctggtgcgt gtttctcagc
      661 gcttcgctca gcggcaggtc gtctggatac atggcatcac tctcccccgg attcgagacc
      721 ttccgacaat tggaacggcc ggccgcagac aggccagagc gtaacggctc ggcaaggaga
      781 ccaccagctg agcgaagcgg gccggtcgtc gcctcaagtc tctgaccgcc cggtgacgtc
      841 gttgacggca ccgacgaaag cgcagtaatc gcaccgccag ctcgcttgcg gtatctccgg
      901 ctggttcagg caggcgtgca gcgccacgat ggtgggctcc acccaatcgt cgttgccctg
      961 gtagggaatc aggtgaacgt cgaagtcgat ctttgcatca aacgcctctg cgtccgggcg
     1021 gccggtgcag tagacgaagt agccggtatc ggacacatcg agcccgttac gccgcaggag
     1081 ccactggtag atctccatct gacgcttgta gccatccatc caggcggctt ccatggaggt
     1141 gacgggctct ttcttggccg tcgccttgta gtccaccacg atgtaggagc catcgccgtc
     1201 aatccacaga tcgtctatgg cgccggtgat gatcaggttg gtgggctcgt ggaggtgctg
     1261 aacccccttg aagttctcgc gccagacgtc gatgttggcg tgcgcggcgg gaatcgcgtc
     1321 gataccgtag gccgcctgca gcggatgctg actaccctct gaccggtgaa cgtcgaactc
     1381 ggttttcagc agcgtgtcca cggcgctgtt gatgttgaaa gggaagccgg gaggacggtc
     1441 ggtgcccagg cgcctgtcga tgtaaaagca acgcgggcac tcaacgaaac ggtcgatctt
     1501 cgaccgggag atcttgaacg gcttcgcgct tcccggctcg tagagattgc ggcgtcgcgg
     1561 cggcttgtat ctcgcgctca tcgcaacggg tcagagcggg ttgtcttgca tgaactcgat
     1621 caccaccgcg gcaagctcct cgccaacgtc gtcctgcagg aagtggccac cgcctctgat
     1681 gatcgtgtgc ttctggccct gcgcgcccgg cacggagtcg atgaagcgtt gctcctggcc
     1741 tttggtcacg ggatccgaat cgctgaaggc ggtcaggaac
//
LOCUS       DBNHOX010000380         7254 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1572492, whole genome shotgun sequence.
ACCESSION   DBNHOX010000380 DBNHOX010000000
VERSION     DBNHOX010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7254)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1572492"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="AC16UZ_08395"
     CDS             complement(<1..744)
                     /locus_tag="AC16UZ_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6246741.1"
                     /translation="MRRVEIEIEGEYDGRSSKRRDYELGADMQDMTLEQMLALLERQR
                     EDYLGEGTVSLAKRVDRLERAVGLLKTHEKRLVEAMTADFGHRSEHQSMLTDVAGSIG
                     PLRHAQKHLARWMRPERRKVGPFPLNVLGAKARVEYQPLGVVGVISPWNFPVNLTFTP
                     LAGILGAGNRCMIKPSEYTPATSEVMAEIFPTAFDELEIAVVTGGPQVGADFSGLPFD
                     HLLFTGATSIARHVMRAASENLVPVTLELG"
     gene            complement(776..1036)
                     /locus_tag="AC16UZ_08400"
     CDS             complement(776..1036)
                     /locus_tag="AC16UZ_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246742.1"
                     /translation="MPDVVALSHRGKEFEIRLADDGALELHVDGCLRKRREPTGREPQ
                     YVWTNVELQWEEHHYIEARYWAGNRRLKVTVNGDGIYDRSLS"
     gene            1129..2499
                     /locus_tag="AC16UZ_08405"
     CDS             1129..2499
                     /locus_tag="AC16UZ_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6246743.1"
                     /translation="MPASPDQPRFARVLKSREVITLALGAMIGWSWVLMTGFWVQTAG
                     SLGTLIAFAAGGLAIALIGLTYSELASALPRAGGEHVYTHRALGHRWSFVCTWALLFA
                     YVNVCLFESIALPTAVEYLLPGIRMGTLWSVLGADVDVGFVIVGAGSALLVTVVNVLG
                     IKAAARLQSAAIGLILLAGVILVSGAVVFGETDNARPWIATPATGILSVLIMVPAMLV
                     GFDVIPQSAEEINLQPARIGKLLIVSVLLAVAWYGLIAFAVATALSKPELATSSMATG
                     DAATALWGDPIAGRLLVLGGVAGILTSWNAFVIGGSRVMFALAESGSIPAIFARLHPR
                     YRTPYVGVITIGVMCCFAPLFGRTVLVWLIDAGSFAVVVAYLFVPLAFLALRRQEPDL
                     PRPFKVSHPRLVGVGAFVLAIGLLTAYLPGSPSALIWPYEWAIVLAWTIVGAVLFLAY
                     GAKHPR"
     gene            complement(2464..4032)
                     /locus_tag="AC16UZ_08410"
     CDS             complement(2464..4032)
                     /locus_tag="AC16UZ_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR6246744.1"
                     /translation="MDLIGWSWLFLVLYLALMLAFGFIGRRRIKSADDFATARESYGP
                     VFLAFAFAATTASGATFVGFPGIAYDAGLPAVWSVFLYPAGVYLGVLICLKVVSNTGH
                     EFGSRSIPEYLGTRYQSDWMRVLVSLFSLLLFFYLAGQLVSGIVMFEIMLGVSPNWAL
                     AITAAVLMVYVVLGGAHADILTDGVQGLIMVAIAVGLVFLFVSGFGVDGGFTGMIDSL
                     ERQDANLVGWLNPDYVLYHSWWSVAAVLLAHIPLGMLPHIGNKLWALEQPSQRRRFIR
                     LAFTFGLALGMLGLGGLLARAILGDALLQPGESSNTALSMLFVELFPGWLAALLGIGI
                     LAAVMSTADGLVVSSSQIIANDLYRCTIVPRFARELPERTVDRQVLIISRIATVAVMA
                     ICTAMAWALADRNVALIVWIGTGGMMAAFAGPLVVGAVWRGVTKAGAFAGLVGGVTVF
                     SIAHGGLVDPGWFEPGALRDAAAWLLREAPNPWSCAAMGEIVSIALTWGVSKLTQPLP
                     SDHLTRMFGPVGQE"
     gene            complement(4065..5183)
                     /locus_tag="AC16UZ_08415"
     CDS             complement(4065..5183)
                     /locus_tag="AC16UZ_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HIR6246745.1"
                     /translation="MAERNRAQNPPPPSRSDNGWLKGRGATKNEASRYLARHSVPVDD
                     GWSANEVDEPDGSRVATELYPDRTVTLITRNASPDIPFDRSINPYKGCEHGCVYCFAR
                     PTHAYLDLSPGLDFETKIFFKTNVRERLVDELGKRRYQCRTIAMGTNTDPYQPAERHR
                     RITRSVLETLLEARHPVSIVTKSQLILRDLDLLQELARQQLATVAVSLTTLSNDLKTR
                     LEPRTAGPGARLRVIRELRGAGVPVGVMVAPVIPFVNDHEIEDIVAAATDAGARTANY
                     ILLRLPLEVSGLFEAWLGVHFPDRAERVMNAIRSARGGKTYDATWGKRMRGEGEVAEL
                     IAARFKVALRRNGILGATQPPLRTDLFRPPGGSQLSLI"
     gene            5333..6115
                     /locus_tag="AC16UZ_08420"
     CDS             5333..6115
                     /locus_tag="AC16UZ_08420"
                     /inference="COORDINATES: protein motif:HMM:NF017434.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease family protein"
                     /protein_id="HIR6246746.1"
                     /translation="MAHRAGPGRALLRAPLLALLMSGCAVTTGSQDNPNAALVSRVQA
                     LEYQVLATQVAVRELAEDQQSGRADLSRQMGDLNTELGRVPEAVGAICASAETAAAAA
                     CDSDQPIQTVVMSGDKMVVGEMERVWLEPPGTTLIARVDTGAHSSSLHAENLVEFERD
                     GEEWVRFDLILDDEIKTLERRVVRYVRVYQQADPEGTRRPVVTMRLRIGDVHDSFEFT
                     LADRSHLDFQLLLGRNFLADMALVDVGKQFVQPPYQPPSTSR"
     gene            6112..>7254
                     /locus_tag="AC16UZ_08425"
     CDS             6112..>7254
                     /locus_tag="AC16UZ_08425"
                     /inference="COORDINATES: protein motif:HMM:NF025758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UUP1 family membrane protein"
                     /protein_id="HIR6246747.1"
                     /translation="MSQKGPFLFLVALLCIAGVGTAAFRHAEYRIPWLPGEQQNVWEI
                     EARIEYMAQGVPTQAFLTLPPNQDGFRVVNETGASSGFGFEVETQRRQRRAHWSKREA
                     NGPQTLFFKLELVRDAEYRVDGPPPAAPPASALDEPYQTAADQIVQAALPVSANARTL
                     AQQLINRMLLVPADQTVSLLRDRFDEATLLANLLVTAGANARTVQVLELEDGRRRQSL
                     KDYVQVWDDGRWYLFNPTVGPVTDLDNLLLWQTDTPSVLEVVGGTRSRVSFSMISQPR
                     SMLALAEPSTGTGFSLHSLPIAEQGMFKLIMLLPVGALVVVFMRIIVGVRTSGTFMPV
                     LIALAFLQTQLLPGLSSFVLVVSIGLMIRSYLSSLNLLLVARIATLV"
BASE COUNT         1224 a         2452 c         2337 g         1241 t
ORIGIN      
        1 cccgagctcc agggtcacgg gcaccaggtt ttccgacgcc gcccgcatca cgtgcctggc
       61 gatggatgtg gctcccgtga acagaagatg atcgaacggc agccccgaga aatccgcgcc
      121 cacctgaggg ccaccggtga ccaccgctat ctccagctcg tcgaaggccg tcgggaagat
      181 ctctgccata acctccgagg tggccggcgt gtactcggac ggcttgatca tgcaccggtt
      241 gccggcgccg aggatgccgg ccaggggcgt aaacgtcagg ttgaccggaa aattccacgg
      301 gctgatgacg cccaccacgc cgaggggctg gtactccacc ctcgccttcg cgccgagcac
      361 gttcagcggg aacggtccaa ccttgcggcg ctccgggcgc atccagcggg ccaggtgctt
      421 ctgagcatgc cgcagcggtc cgatggagcc ggcaacgtcg gtaagcatcg actgatgctc
      481 gctgcgatgg ccgaagtccg ccgtcatggc ctctaccagc cgcttctcgt gcgtcttgag
      541 gagacccaca gcccgctcga ggcgatccac gcgcttggcc aacgaaacgg ttccttcccc
      601 aaggtaatcc tctcgctggc gctcgagaag cgccagcatc tgctcaaggg tcatgtcctg
      661 catgtctgct cccagttcgt aatctcggcg tttcgaggac cggccatcat actcgccttc
      721 aatctcgatc tcgacgcgcc tcatcggccc gtctgcaggg ggcggcgctc atccctcaag
      781 acagcgatcg gtcgtaaatc ccgtcaccat tgaccgtcac cttcaggcgt cggttccctg
      841 cccagtaacg ggcctctatg taatggtgct cctcccactg gagctcgacg ttggtccaga
      901 cgtactgagg ctcccggccc gtcggctcgc gacgcttgcg caggcacccg tcgacatgga
      961 gttccagggc gccgtcgtcc gccaggcgga tctcgaactc cttgccgcga tggctcaatg
     1021 cgacgacgtc tggcaaacca ggctccaggt gttgaaatcg agaggtcgag ggaggcggca
     1081 attaccgcta agcttgcggg ctcgaattct gcccgcaggc gccacgcgat gccagcaagc
     1141 cctgaccaac cccgctttgc ccgagtgctg aagagccggg aagtcatcac cctggcgctg
     1201 ggcgccatga tcggctggag ctgggttctg atgaccgggt tctgggtgca gacagccggc
     1261 agcctgggca cgctcatcgc cttcgccgcg ggcggtctgg ccatcgcgct gatcgggctg
     1321 acctacagcg agctcgcctc cgccttgccg cgcgcgggcg gcgagcacgt ctacacccat
     1381 cgcgcgctgg gacaccgctg gtcgttcgtt tgcacctggg ccctcctgtt tgcctacgtg
     1441 aacgtgtgcc tgttcgaatc catcgctttg cccacggcgg tggagtatct gctgcccggt
     1501 attcgcatgg gtaccctctg gagcgttctc ggtgcggacg ttgacgtcgg cttcgtgatc
     1561 gtgggcgcgg gcagcgcgct tctggtcacc gtggtcaacg tgctgggcat caaggccgcc
     1621 gcgcggctgc agagcgcggc catcgggctg atcctgctgg ccggcgtgat tctcgtcagc
     1681 ggtgcggtgg tcttcgggga gaccgacaat gccaggcctt ggatcgccac gccggccacc
     1741 gggattctca gcgtgctgat catggtgccc gccatgctgg tgggctttga cgtgattccc
     1801 cagtccgccg aggagatcaa cctccaaccc gcccgcatcg gcaagctgct catcgtatcc
     1861 gtgctcctcg cagtagcctg gtacgggctg atcgccttcg ccgtcgccac ggcgctaagc
     1921 aaacccgagc tggccacgag ctccatggcc acgggcgacg ccgccaccgc tctctggggt
     1981 gaccccatcg ccgggcgcct cctcgttctc ggcggcgtgg ccggcatcct caccagctgg
     2041 aacgccttcg tgatcggggg cagccgcgtg atgttcgctc tggcggagtc aggctccatc
     2101 ccggccatat tcgcccggct gcatccccgc taccggacgc cctacgtcgg ggtcatcacc
     2161 atcggcgtga tgtgctgctt tgcgcccctc ttcggacgca ccgtgctggt ctggctcatc
     2221 gatgcgggga gcttcgccgt ggtggtggct tacctgttcg tgcccctggc ctttctggcc
     2281 ctgcgacgcc aggagcccga cctgccccgg ccgttcaagg tctcccatcc gcgcctggtg
     2341 ggtgtcggcg ccttcgtgct cgccatcggc ctgctcaccg cctacctgcc cggcagcccg
     2401 tctgcgctca tctggcccta cgagtgggcc atcgtgctcg cctggaccat tgtcggcgcg
     2461 gtgctattcc tggcctacgg ggccaaacat ccgcgttaga tgatctgacg gcaggggttg
     2521 ggtgagcttg gacacgcccc aggtcagcgc gatggacacg atctccccca tggccgcgca
     2581 ggaccagggg ttgggcgctt cccggagaag ccaggcggcg gcatcgcgca gcgccccggg
     2641 ctcgaaccag ccggggtcga cgaggccgcc gtgggcaatg gagaaaacgg ttaccccgcc
     2701 caccagaccg gcgaacgcgc ccgccttggt cacgccgcgc cagacggccc caaccaccag
     2761 cgggcccgcg aatgccgcca tcatgccccc cgttccgatc cacacgatca gggcaacgtt
     2821 tctgtcggcc agggcccagg ccatggcggt gcagatggcc attaccgcca cggtcgctat
     2881 gcggctgatg atgagaacct gcctgtccac agtgcgctcg ggcagctcgc gcgcaaaccg
     2941 gggaacgatg gtgcagcggt aaaggtcgtt ggcgatgatc tgcgacgacg aaaccaccag
     3001 gccgtcggcc gtggacatca ccgccgcgag gatgccgata cccagcaggg cggcgagcca
     3061 tcccggaaaa agctccacga agagcatgga cagcgccgtg ttgctcgact cgccgggctg
     3121 cagcagggcg tctcccagaa ttgcccgcgc gagcaggccg cccagcccca gcatccccag
     3181 cgcgaggccg aaggtgaatg ccagccggat gaatcggcgg cgctggctgg gctgctccag
     3241 cgcccacagc ttgttgccga tatgcggcag catgcccaag gggatgtggg cgagcagcac
     3301 cgcagcgaca gaccaccagg agtgatagag gacgtagtcc gggttcagcc agcccaccag
     3361 gttcgcatcc tggcgctcga ggctgtcaat catgcccgtg aagccgccgt cgactccgaa
     3421 gcccgagacg aagaggaaga cgagccccac cgcgatggcc accatgatca gtccctgcac
     3481 cccgtccgtg aggatgtcgg catgagcccc gcccagcacg acgtagacca tcagcaccgc
     3541 cgcggtgatc gccagcgccc agttcgggga gacgccgagc atgatctcga acatcacgat
     3601 cccggagacc agctgacctg ccaggtagaa gaagagcaga agggaaaata gagagaccag
     3661 aacccgcatc cagtccgact ggtatcgggt gccgaggtac tccggaatgc ttctgctgcc
     3721 gaactcgtgc ccggtgttgc tgacgacctt gaggcagatc aggaccccga ggtagacgcc
     3781 tgcggggtaa agaaagacgg accatacggc aggcagcccc gcgtcatagg cgatacccgg
     3841 gaagccgacg aaggtcgcgc cgcttgccgt ggtggccgca aaggcgaaag cgagaaacac
     3901 gggcccgtag gattcccgcg cggtcgcgaa gtcgtcggcg cttttgatcc gccttctgcc
     3961 gatgaagccg aaggcgagca tcagcgccag gtagagcacc agaaataacc atgaccagcc
     4021 gatcagatcc atgctcaccc tccccgccga gcggttaaag cttatcagat caggctcagc
     4081 tggctgcctc cggggggtcg gaagagatct gtgcgaagag gcggctgggt ggcgccgaga
     4141 atgccattgc gcctgagcgc gaccttgaag cgcgcggcga tgagctcggc aacttctccc
     4201 tcgccccgca ttcgcttgcc ccacgtggcg tcgtaggtct tgccgcctcg ggccgagcgt
     4261 atcgcgttca tgacacgctc ggcgcgatcc ggaaagtgca cgcccagcca ggcttcgaag
     4321 aggccgctca cctccagcgg caggcgcagc aggatgtaat tcgccgtgcg agcaccggca
     4381 tcggtagccg cggccacgat gtcctcgatc tcgtgatcgt taacaaaggg gatgacgggc
     4441 gccaccatga ccccgacggg aacgcctgcg ccccgcagct ccctgatgac cctgagcctg
     4501 gccccaggcc ccgccgtgcg cggctcaagg cgggtcttca gatcgttgct cagcgtcgtg
     4561 aggctcaccg ccacggttgc cagctgttgg cgagccagtt cctgaaggag gtcgaggtcg
     4621 cgcagtatga gctggctctt ggtcacgatg ctcaccggat gccgggcttc cagcagcgtc
     4681 tccagcacgc tccgggtgat ccgccggtgc cgctcggcgg gctggtacgg atccgtgttg
     4741 gtccccatgg cgatcgtgcg gcactggtag cggcgctttc ccagctcgtc gacgagccgc
     4801 tcgcggacgt tcgtcttgaa gaagatcttc gtctcgaaat ccagccccgg cgagagatcc
     4861 aggtaggcgt gagtgggccg ggcgaagcaa tagacgcagc cgtgctcaca gcccttgtag
     4921 ggattgatgg aacggtcgaa ggggatgtcc ggcgacgcgt tgcgggtgat cagcgttact
     4981 gttctgtccg gatagagctc cgtggccacc ctggacccgt ctggctcatc gacttcgttg
     5041 gccgaccagc cgtcgtccac gggcacgctg tgccgggcga gatagcggct cgcctcgttt
     5101 ttggtagcgc cgcggccctt caaccagccg ttgtccgacc ggctgggcgg cggcggattc
     5161 tgcgcgcgat ttctctctgc cattgaaggg ccttccaaca gacaggcaga ataactgtat
     5221 atatgtacag tatcaagcgt cggataccga cagtagactt cgacgccgaa gcccaaactg
     5281 actgcagatg aaccgaccga gaacggcctt tgaagacgtt cggcgcggcg ctatggctca
     5341 ccgcgccggt ccgggccgcg ccctgctgcg ggcgccgctg ctggcgctgc tgatgagcgg
     5401 atgcgcagtc accaccggca gccaggataa tcccaacgcg gcgctggtca gccgcgtgca
     5461 ggcgctggag taccaggtgc tggcgaccca ggtcgcggtc agggagctgg ccgaggatca
     5521 gcagtcgggc cgggccgatc tctccaggca gatgggcgac ctcaacacgg agcttggccg
     5581 cgtgcccgag gccgtcggcg cgatctgcgc cagcgcggag accgccgcgg cggcggcctg
     5641 cgattccgac cagcccatcc agaccgtggt catgagcggc gacaagatgg tggtcggcga
     5701 gatggagcgg gtttggctgg agccgcccgg cacgaccctc atcgcccgtg tcgacaccgg
     5761 tgcccattcc agctccctgc atgcagagaa cctggtggaa ttcgagcgcg acggcgagga
     5821 atgggtgcgc ttcgacctga ttctggacga cgagatcaag actctggaac gccgggtggt
     5881 ccgctacgtt cgcgtctatc agcaggccga tcccgaaggc acccgccgac cggtggtcac
     5941 catgcgcttg cgtatcggcg acgtgcacga ctccttcgag ttcaccctgg cggaccggtc
     6001 gcacctagat tttcagctgc tgctgggacg taacttcctc gccgacatgg cgctggtgga
     6061 cgtcggcaag cagttcgtcc agccgcccta ccagcccccg tcaacctcca gatgagccaa
     6121 aaggggccat ttctcttcct ggtggccctc ctgtgcatcg ccggcgtcgg cacggccgcg
     6181 ttccggcacg ccgagtaccg gataccctgg ctgcctggcg agcagcagaa cgtctgggag
     6241 attgaggcgc gcatcgagta catggcccag ggcgtcccga cccaggcctt tctgacattg
     6301 ccccccaatc aggacggctt cagggtggtc aacgaaaccg gcgcatcgtc aggcttcggc
     6361 ttcgaggtag agacccagcg ccggcagcgc agggcacact ggtccaagcg ggaggcgaac
     6421 gggccgcaaa cccttttctt caagctggag ctcgtgcgcg atgcggaata ccgggtggac
     6481 ggccccccgc cggcagcgcc gccggccagc gccctggacg agccgtacca gacggcggcc
     6541 gatcagatcg tccaggccgc cctgccagtc agcgccaacg cgcgcaccct ggcacaacag
     6601 ctgatcaacc gcatgctgct ggtgcccgcc gaccagaccg tcagccttct gcgcgaccgc
     6661 ttcgacgagg cgaccctgct ggccaacctg ctggtcactg ccggcgcgaa cgcgcggacg
     6721 gtgcaggtgc tggagctcga ggatggacga cgccgacaga gcctcaaaga ctacgttcag
     6781 gtatgggacg acggccgctg gtacctcttc aaccccacgg tagggccggt gacggacctg
     6841 gacaatctcc tgctatggca gaccgacacg ccctcggtgc tggaggtggt tggcgggacg
     6901 cgaagccgcg tgtctttctc catgatcagc cagccccgct ccatgctggc cctggccgag
     6961 ccttccacag ggaccggctt ctcgctgcac agcctgccca tcgccgagca gggcatgttc
     7021 aagctgatca tgctgctgcc ggtcggggcg ctggtcgtgg tcttcatgcg catcatcgtg
     7081 ggggtgcgca cgtcgggcac cttcatgccc gtgctgatcg ccctggcatt cctgcagaca
     7141 caactgctgc ccgggctgtc gagcttcgtc ctggtcgtgt ccatcggcct catgatccgc
     7201 tcttacctct ccagcctgaa cctgctgctc gttgccagga tcgcaacgct ggta
//
LOCUS       DBNHOX010000381         2601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1058851, whole genome shotgun sequence.
ACCESSION   DBNHOX010000381 DBNHOX010000000
VERSION     DBNHOX010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1058851"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="AC16UZ_08430"
     CDS             <1..896
                     /locus_tag="AC16UZ_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6246748.1"
                     /translation="EQQKEEWLRPLLEGKIRSAFAMTEPQVASSDAANISLEARLEGD
                     EWVLNGEKWWTSGIGDPRCKIMIVMCVTEPDAPRHSRQSQILVPVETDGVEVLKMMHV
                     FGYADAPHGHGHVRFTDVRVPKDNIILGSGRGFEIAQGRLGPGRIHHCMRSIGVAERA
                     LELMCRRALSKEAFGKPIADLGGNYDKIADARINIEMARLLTLKAAYMMDTVGNKIAR
                     SEIAQIKVAVPNIALKVIDDAIQIHGGAGVSQVFPLAKMWAGQRTLRLADGPDEVHRR
                     AVARLELGKYPDKDPIRTDVW"
     gene            909..1823
                     /locus_tag="AC16UZ_08435"
     CDS             909..1823
                     /locus_tag="AC16UZ_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246749.1"
                     /translation="MGRLDDKVAVITGAGRGIGREIALMMAGEGAKIVVNDLGGNMDG
                     TGTGMIADDVVAEIRAAGGEAVSNTASVAEVAGGESLLQSALGAFGAMDILVNNAGIL
                     RDRTIFKMEEADWDAVQAVHLKGHYCCTRPFARYIRENNRTGCRVLNFSSVSGLYGNF
                     GQANYGAAKAGIAGFSRVLALELAKYGCTVNTISPGALTRMTIPLREARGEEVKDEEI
                     SESGPQHVAPICVWLATEAAAGITSQIFHTARGGVGIMQQPRVIKQYHKSSGLWTLDE
                     LDGIVPELVAARAANEAAAKDSGQPIEV"
     gene            1973..2311
                     /locus_tag="AC16UZ_08440"
     CDS             1973..2311
                     /locus_tag="AC16UZ_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR6246750.1"
                     /translation="MGIQRAAIMIIVGALAWATANGAYAGKIVSWVDEKGVTHFTDAG
                     LANGQTNAVTVATSDTPVRAAPEDIVPERKSTPTVAQSGSGKSEGQKSGLRGYRSSWA
                     RDPDRFKNLN"
     gene            complement(2363..>2601)
                     /locus_tag="AC16UZ_08445"
     CDS             complement(2363..>2601)
                     /locus_tag="AC16UZ_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246751.1"
                     /translation="LLVQTVTLIGNLNDGSLLLQTQINFDPGDNQRLELGVVEPLGGS
                     GDEFGGVPVVADPAGSRLTVGGGSRLYARWVYYF"
BASE COUNT          529 a          822 c          858 g          392 t
ORIGIN      
        1 ccgagcagca gaaagaggaa tggttacggc cgctgctgga aggaaagatc cgctcggcgt
       61 tcgcgatgac cgagccgcag gtcgcttcct cggatgccgc caacatatcc ctggaagcga
      121 ggctcgaagg ggacgagtgg gtgctcaacg gcgagaagtg gtggacctcg gggatcggcg
      181 acccccgctg caagatcatg atcgtgatgt gcgttaccga gccggacgcg ccacgacact
      241 cgcggcagtc gcaaatcctg gtgccggtgg aaaccgacgg cgtcgaggtc ctgaagatga
      301 tgcacgtctt cggctacgcc gacgcgcccc acggacacgg gcacgtccga ttcaccgacg
      361 tccgagtacc aaaggacaac atcattctcg ggtccggtcg cggtttcgag atcgcccagg
      421 gccgactggg tccggggcgc atccaccact gcatgcgttc tatcggcgtc gccgagcggg
      481 ccttagaact gatgtgccgc cgcgcgctct ccaaagaagc gttcggcaag cccatcgccg
      541 acctaggcgg caactacgac aagattgccg atgcgcgcat caacatcgag atggccaggc
      601 tgctgacgct gaaggctgcc tacatgatgg ataccgtggg caacaagatc gccaggagcg
      661 agatcgccca gatcaaggtg gcggtaccca acatcgcgct gaaagtcatc gacgacgcca
      721 ttcagatcca tggtggcgcc ggcgtatccc aggtatttcc gctggccaag atgtgggcag
      781 gacagagaac tctacgcctt gccgacggcc ccgacgaagt gcaccggcgc gcggtggcca
      841 ggctggagct gggcaaatac ccggacaaag atccgatccg aacggacgtt tggtaggagg
      901 cgatcgtcat gggccgactg gatgacaagg tggccgtcat caccggggcg ggtcgcggga
      961 tcggccgcga gattgcgctg atgatggccg gtgaaggtgc gaaaatcgtc gtcaacgatc
     1021 tcggcggcaa catggacggg accggaaccg gaatgatcgc cgacgacgtg gtggccgaga
     1081 ttcgcgcggc cggcggcgag gccgtctcga acaccgcatc cgtcgccgag gtggcgggcg
     1141 gcgagagcct tctgcagtcg gccctgggcg cgtttggcgc catggacatc ctggtcaaca
     1201 acgcggggat attgagggac cggaccatct tcaagatgga ggaggcggac tgggacgcgg
     1261 ttcaagccgt gcacctgaaa ggccactact gctgcacccg ccccttcgcg cgctacatcc
     1321 gggaaaacaa ccgcaccggc tgcagggtcc tcaatttttc cagcgtgtcc ggtctctacg
     1381 gcaacttcgg acaggccaac tacggagcag ccaaggcagg catcgcaggg ttttcccggg
     1441 tgctggcact ggaacttgcg aagtacggct gcacggtgaa caccatctcg cccggggcgc
     1501 tgacccgcat gaccatcccg ttgcgggagg cgcgcggcga ggaggtgaag gacgaggaga
     1561 tcagcgagag cgggccgcag cacgtcgccc cgatctgcgt gtggctcgca acagaggcgg
     1621 ccgccggcat cacgtcccag atattccaca ccgcgcgcgg cggggtcggc atcatgcagc
     1681 agccccgggt catcaagcag taccacaagt cgagcggcct gtggacgctc gatgagctcg
     1741 acggcatcgt tcccgagctg gtcgccgcgc gggcggccaa cgaggccgcc gccaaggatt
     1801 ccgggcaacc catcgaggtc tgagggaaaa atcccctgag cggctggctc accggccgct
     1861 cacaactatc ctcgaacccc cgacaaagcg gggacaactc gccggcgcgc ctgtgacaac
     1921 ataggcgctg actctctaaa cccagactgt agtgaccgag gagggttttc ccatgggaat
     1981 tcagcgagca gccatcatga tcatcgtcgg cgccctggcg tgggcgacgg caaacggcgc
     2041 ctacgccggc aagatcgttt cctgggtgga cgaaaaaggt gtgacgcact tcacggatgc
     2101 cggccttgcc aacggccaga cgaatgcggt gaccgtggct acctccgaca cgcccgtccg
     2161 cgccgccccg gaggacatcg tccccgagcg gaagagcacg ccaaccgttg cccaatcggg
     2221 gtccggcaag tcagagggcc agaaaagcgg cctgcgcggc taccgcagca gctgggcgcg
     2281 cgatccggac cgcttcaaga atctcaactg aggatcatcc gtttttgtga ggcgccagcg
     2341 ggtcagaccg tctgcccgcg cctcagaaat agtagaccca ccgcgcgtaa aggcgcgacc
     2401 cgccccctac ggtgagacgg gagcccgccg gatccgcgac cacgggcacc ccgccgaact
     2461 cgtctcctga accgccgagc ggttcgacca ctccgagctc gagacgctgg ttgtcgccag
     2521 ggtcgaagtt gatctgcgtc tgcagaagca ggctgccgtc gttgagattg ccaatgagcg
     2581 tcacggtctg aaccagcaga g
//
LOCUS       DBNHOX010000382        16134 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_801209, whole genome shotgun sequence.
ACCESSION   DBNHOX010000382 DBNHOX010000000
VERSION     DBNHOX010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 16134)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16134)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16134
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_801209"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="AC16UZ_08450"
     CDS             <1..330
                     /locus_tag="AC16UZ_08450"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine aminopeptidase domain-containing protein"
                     /protein_id="HIR6246752.1"
                     /translation="VNAENPEFIEFLKHHDPLQARVLPVQWVTAMVAWKKRFVSRAPS
                     DLGLLVVQGTADRTVDHRYNLKIIEQLFDAKVVYVPEARHHLVNESVVLRQQIFQAVD
                     AQLDAAG"
     gene            338..1714
                     /locus_tag="AC16UZ_08455"
     CDS             338..1714
                     /locus_tag="AC16UZ_08455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013974677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6246753.1"
                     /translation="MTSAHGQLTSALSPSQVRTDPDSLHEWGRDWTRTAEPAPAAVVF
                     PETTADVVAIVRLANSEGLKLVPSGGRTGLSGGAVAADGEVVVSFDRMNRVLEFSAAD
                     RLLSCQAGVVTRTVQDFARERGLSYPVDFASSGSSQIGGNIATNAGGIKVIRYGLTRD
                     WIAGLRVVTGAGELLDCNRGLVKNATGYDLRHLFVGSEGTLGFIVEADVRLAPAPAPQ
                     TVMLLGVTEFPRLIDVLSVLQSTVVLSAFEFFSELALQKVLSHRKLRRPLAETAPFYA
                     LVEFDTADLEPATAAYEAAVGGGWVCDGVVSQSEGQAAALWSLREGISESIAQFTPYK
                     NDLSVRVSVLPEFLAQVDGLVAQSYPGFEVCWYGHIGDGNLHLNILKPGGLSLAEFYA
                     RCHAISPSLFELVARHGGSVSAEHGVGLLKRDFIGYSRSAAEIEIMRALKGVLDPNQI
                     MNPGKLLS"
     gene            complement(1749..2414)
                     /gene="rpiA"
                     /locus_tag="AC16UZ_08460"
     CDS             complement(1749..2414)
                     /gene="rpiA"
                     /locus_tag="AC16UZ_08460"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIR6246754.1"
                     /translation="MDQDQLKRSAAEAALAYIEPKLGSASIVGVGTGSTTNHFIDALG
                     AIRHKFDAAVASSEASAARLKAVGIPVIDLNAAAAVAVYVDGADEVNAARQLIKGGGG
                     ALTREKIVAASADEFVCIVDESKVVQTLGAFPLPVEVIPMARGLAARAIVGLGGAPEL
                     REGFVTDNGNIILDVYELTITDPAAMESAVNNIVGVVCNGVFAAQAADLVIVAGASGV
                     TSF"
     gene            2487..3998
                     /gene="ilvA"
                     /locus_tag="AC16UZ_08465"
     CDS             2487..3998
                     /gene="ilvA"
                     /locus_tag="AC16UZ_08465"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HIR6246755.1"
                     /translation="MEKYVKKILSSRVYEAAVETPLQPARNLSIRLGNHVRLKREDMQ
                     PVFSFKIRGAYNRIVQLTEEERGRGVIAASAGNHAQGVAYSAQQLGIKALIVMGRNTP
                     SIKVNAVRALGAKVVLHGDAYDEAAAHALKLAERNGYVFVHPYDDPDVIAGQGTVGVE
                     ILNQHVAVPDAIFVPVGGGGLIAGIAAYVKYLAPDTRIIGVEAEGSACLAAAMAAGRR
                     VRLPFESLDLFADGVSVAQIGKETFKVARRCVDEVITANTDEICAAIKDLFDDTRSIA
                     EPAGALAVAGLKKFVEREGVRNLNLVAVVSGANVNFDRLRHISERAEVGEHREVVLGV
                     TIPEKPGSFLKFCKMLGNRPVSEFNYRFATDREAQVYVGLQVSAADDRAELMKSLSTG
                     YVVHDMTDNEAAKLHVRHMVGGRTFGSKPELLYRFEFPERPGALLRFLTGLGSRWNIT
                     MFHYRNHGAAWGRVLVGFEAQPRDRRSLGEYLKRIGYRYWEETDNPAYQLFLR"
     gene            complement(4005..4538)
                     /gene="def"
                     /locus_tag="AC16UZ_08470"
     CDS             complement(4005..4538)
                     /gene="def"
                     /locus_tag="AC16UZ_08470"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR6246756.1"
                     /translation="MAVRKIIRMGHPTLRRVARPLAGEEIRSEAVGRLIGDMIDTLHD
                     YGGIGLAAPQINESVRLAIIEIPGGPTRYGEIPALPLTVFINPSVEVVDPATAGYWEG
                     CLSVPGLRGFVERPQHVRVNALDQEGKPLDLELKGFLATVFQHEFDHLDGRLYVDHLK
                     DPALLAFDEEFERYLAE"
     gene            complement(4558..5142)
                     /locus_tag="AC16UZ_08475"
     CDS             complement(4558..5142)
                     /locus_tag="AC16UZ_08475"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR6246757.1"
                     /translation="MTSTLERQELRRFFRERRRALGSHQQREHALRVARHLLNNPVSW
                     RSRRIGAYWANDGEVSLAPLLGELEYRNRQLALPVVSTAGRMAFYRYHRAAPMLVNRY
                     GIPEPGPGSAFVDGRSLDLILLPLVAFDEYGVRLGMGAGYYDRFLGALPEPLRPRIVG
                     IAHEVQRSADPLPFQDWDIPLDGIVTERGWQAFT"
     gene            complement(5164..5374)
                     /gene="ssrS"
                     /locus_tag="AC16UZ_08480"
     ncRNA           complement(5164..5374)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC16UZ_08480"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(5447..5761)
                     /locus_tag="AC16UZ_08485"
     CDS             complement(5447..5761)
                     /locus_tag="AC16UZ_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR6246758.1"
                     /translation="MSEEATTVSVKILDKEYQVSCPAEEVDALSRSARYLDEQMRSIR
                     ESGKIFGMDRIAVMAALNIANEFINSSKSLESTHGVVSQKVTELTERVGRALSEQKQL
                     NL"
     gene            complement(5775..5981)
                     /locus_tag="AC16UZ_08490"
     CDS             complement(5775..5981)
                     /locus_tag="AC16UZ_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HIR6246759.1"
                     /translation="MADTEWTALESKVDELIELCSVLTKENRALRAQQQNWTTERAKL
                     IEKNELAKARVESMITRLKALEQD"
     gene            6103..6645
                     /locus_tag="AC16UZ_08495"
     CDS             6103..6645
                     /locus_tag="AC16UZ_08495"
                     /inference="COORDINATES: protein motif:HMM:NF015642.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HIR6246760.1"
                     /translation="MADLTALALGAVESLSVAEIHGAVCGLAVASEGGIELGSLVELV
                     GIEALRDEHSVGEFVTAAVDALHAEDLSFAPLLPDDHVDLALRLEALGEWCGAFLAGF
                     ASGSRFQELGEEQIPEEAREIIGDFTYIANVDGSEAGDGEDPESAERDYAEVHEYVKV
                     GALLLRTLLGGDAPEETARP"
     gene            6642..7970
                     /gene="pepP"
                     /locus_tag="AC16UZ_08500"
     CDS             6642..7970
                     /gene="pepP"
                     /locus_tag="AC16UZ_08500"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HIR6246761.1"
                     /translation="MIAAAEYRRRRQRLMSLMDEGSIAVLPAAGQRFRNRNTEYPFRQ
                     DSDFYYLTGFAEPDAVLVLAPGRAHGQVILFCREREARAERYDGERVGPERAAERIGV
                     DDAFPVNDLEEILPGMLEGRERIYITLGEYPEFDNRLMHWVAGIRAREAGGAIPPGEF
                     VALKHLLHEQRLYKSAAELRAMREAARITCSAHRRAMAACRPGMTETQLEAELLHEFM
                     VNGARSPAYPSIVGAGANACVLHYVDNHDVLKKGDLVLIDAGCEYQHYASDVTRTFPV
                     AGKFSAAQRALYEVVLEANRQGIEACRPGATFIEPHSTALKVMVEGLIELNLIQGDVE
                     EILQSERYREFCPHNSSHWLGSDVHDVGDYRVEGAWRPLEPGMVLTVEPGIYIPADDS
                     TRHLAPRWRGVGIRVEDDVAITRQGHEVLSASAPKSVDDIERLMRGGADG"
     gene            7963..9144
                     /locus_tag="AC16UZ_08505"
     CDS             7963..9144
                     /locus_tag="AC16UZ_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6246762.1"
                     /translation="MADVVVVGGGLIGALAALGVADGGRRVTVLERREPERQRGRLGV
                     DIRNVAVSPASRELLEAVGVWSSLEPAAYQRMEVWEERGTRGMHFDAAEVGRAELGWI
                     LESSQVVRSLWDLLQRHRNVRLEVGADLASLRLAASGAVLETDRGDIRARLVVAADGA
                     RSRVRELLAVPAEEAPTGHHALATVVRTEQEHEGTCLQRFLLEGPLALLPTADAHLSS
                     VVWSQPAEEAERRAKESDGDFCLELERCLEARFGKVEAVDARVVFPLAQTLVSTFNPH
                     HRVLLIGDAARVVHPLAGMGANIGFEDVRDLLAELAGIGGDQDPGAPGIWRRFARQRR
                     LRSQMVLALMSGFRRAYSADGPLASWVRNTGVGWLDRAQPLKAQIMREALGVGPLARR
                     W"
     gene            9233..10300
                     /gene="gcvT"
                     /locus_tag="AC16UZ_08510"
     CDS             9233..10300
                     /gene="gcvT"
                     /locus_tag="AC16UZ_08510"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017530883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR6246763.1"
                     /translation="MTRRTPLYELHVGLGAKMVDFAGWEMPLHYGSQLDEHHAVRRHV
                     GLFDVSHMTIVDVSGARSLDFLQRLVANDVARLEAPGQALYGVLLNPAGGVIDDLIVY
                     RRDADFRLVVNAATREKVLNWLAGSNEEAVNILERDLAMLAIQGPAAVEMYQSLPDSL
                     EVRGLKAFTAAVRGEEMIARTGYTGEDGLEIMAPGDRARQLWLELIGSGAQPAGLGAR
                     DTLRLEAGLNLYGQDMDDETSPLVSNLAWTIAWEPSGRNFIGRQALEDERARGIAERL
                     TGIVLEDKGVIRRGCRVVTAAGDGHVTSGIFSPTLGYSIGLARVPKGAKGPCEVEMRG
                     RLKQARIVRPPFVRNGLQVFE"
     gene            10297..10692
                     /gene="gcvH"
                     /locus_tag="AC16UZ_08515"
     CDS             10297..10692
                     /gene="gcvH"
                     /locus_tag="AC16UZ_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR6246764.1"
                     /translation="MSDVPQELKYARTHEWARLEEDGTITVGISDHAQDALGDVVYVE
                     HPEPGQPVAAGDEAGVVESVKAASDIYAPVSGSVIAVNEALDDAPETVNQDPYGDGWF
                     FKIQPDDVAELDELLDSEAYAELCENEEH"
     gene            10724..12082
                     /gene="gcvPA"
                     /locus_tag="AC16UZ_08520"
     CDS             10724..12082
                     /gene="gcvPA"
                     /locus_tag="AC16UZ_08520"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HIR6246765.1"
                     /translation="MPFIPHTAEEVQVMLSQIGVARTEDLFDEIPQNLRAGALSSVPA
                     GASELEMLRKLGARAALDESVVCFAGAGSYDHHIPAAVWDLTSRGEFMTAYTPYQAEA
                     SQGTLQLIYEYQSMMTALTGLDVSNASVYDGASGLAEAVLMAVRSNKKARSRTVLLPD
                     SLHPHYAAAARNICRHQSVEFVGMALDHDGRVDLEALETDGPDEVCAVVVQQPNFFGM
                     LEDVDALTDWAAERGGLVIAVVNPLALAVLKPPGEWGGSGADIACGEGQPLGVPMSSG
                     GPAFGFICCRSALVRQLPGRVIGRTEDLTGKPGFTLTLQAREQHIRRGKATSNICTNQ
                     GLLVTAATIHMSLLGPVGLARIAEHCHANTRALVEALTAIDGVEARHQGPFFHERVLQ
                     LPRRADEVVAGLLERGILGGVALGAYHESMNHDLLVCATEKRTDDEIGRYAAALREVI
                     AR"
     gene            12079..13542
                     /gene="gcvPB"
                     /locus_tag="AC16UZ_08525"
     CDS             12079..13542
                     /gene="gcvPB"
                     /locus_tag="AC16UZ_08525"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIR6246766.1"
                     /translation="MSDVRTIFELSAQNRGAAAQHLSERPGAMDDIPASLRRERAPGL
                     PEVSELQVVRHYTNLSRKNFSIDTNFYPLGSCTMKYNPRGAHRAASLPGFLNRHPLAP
                     DSASQGFMACLHELQEILKDVTGMQGVSLTPMAGAQGEFAGVAMIRAYHEARGDHARN
                     EILVPSAAHGTNPATAAMCGYRVAEVPVNASGDVDLEALKLAVGPQTAGLMMTNPSTC
                     GVFERGIESIAAVVHDAGGLLYYDGANLNAILGKVKPGSMGFDVLHMNLHKTFATPHG
                     GGGPGAGPVGVGERLLPYLPVPFVAQDAKGAYRWVTDAELPDSIGRLSAYAGNAGILL
                     RALAYALMLGREGMHRVGEYSTLNANYLARRLEDAGFELAYPDRRATHEFILTFSESA
                     RSRDVTAMDFAKRLLEYGIHSPTTYFPLLIPECFLIEPTETETKAELDAFVDALVAIQ
                     REAEADPEKVKGAPYDLPVRRLDDVKAARELDLAWRG"
     gene            complement(13539..15614)
                     /locus_tag="AC16UZ_08530"
     CDS             complement(13539..15614)
                     /locus_tag="AC16UZ_08530"
                     /inference="COORDINATES: protein motif:HMM:NF015168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR6246767.1"
                     /translation="MSNRLADETSPYLRQHADNPVDWHPWDEAALALASEQQKPILLS
                     IGYSACHWCHVMAHESFEDPATARVMNEHFVNIKVDREERPDLDKVYQLAHQLLTQQG
                     GGWPLTMFLDPDTRLPFFGGTYFPKTPRYQLPGFVDLLLRIAETFASQRDELRDHGTR
                     VADVLGRLAGGDSQAGELGDAELLAAAREQLGAQADEDEGGFGGAPKFPMPETVTYLL
                     RHWAYAQRSGEADRQGLEFVMTTLTRMARGGIYDHLGGGFCRYATDRRWMIPHFEKML
                     YDNGQLLALYSDALAIGPDELFSQAVSDTADWLLREMRHPQGGFYAALDADSEGEEGK
                     FYLWRRDQVKRLLDEDEYLVVETLYGLDKPANFEGKWNLHRYDAWRSVVERLSMDRAA
                     ANDLLASARRKMLAARELRVRPGLDDKVLTAWNGLAIKGLARAGSRLGQSRWLDAAAQ
                     AADFARARLWRDGMLYATWQGAPRYPAYLDDYANLLDGLIALLGARWREEDAAFACKL
                     ADGALERFYDADRGGFFFTAHQHETLIHRPKPTMDDALPPGNGVMVQALTALGHLFGE
                     TRYLEAAAATLAWARAAMEQYPAGHCCLLSGLQDEIMPPELVVVRGAAATFPDWRRAA
                     VSGYTPWRAVYCIPFEGVSTLPAYLPGPVSDDPHSQTVAYVCSGLSCSLPIGTPEDLG
                     EALRSRPRV"
     gene            15756..>16134
                     /locus_tag="AC16UZ_08535"
     CDS             15756..>16134
                     /locus_tag="AC16UZ_08535"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HIR6246768.1"
                     /translation="MVDADGFRPNVGIVLSNSIGQVLWARRVGGQDAWQFPQGGIREN
                     ESVQAALFRELFEEVGLVADDVEVLASTRGWLRYRLPRRMRRYNSSPGFQGQKQKWFL
                     LRMLGDDARVQMDGSAKPEFDSWR"
BASE COUNT         2693 a         5104 c         5513 g         2824 t
ORIGIN      
        1 gtcaacgccg agaatccgga gttcatcgag ttcctcaagc accacgaccc gctgcaggcg
       61 cgcgtgctgc ccgttcagtg ggtcaccgcc atggtggctt ggaagaagcg cttcgtcagc
      121 cgcgcgccaa gcgatctggg gctgctggtg gtgcagggca ccgccgaccg cacggtcgac
      181 catcgctaca acctgaagat catcgagcag ctcttcgatg ccaaggtggt ctatgtcccc
      241 gaggcgcgac accacctggt caacgagtcg gtggtcctcc gccaacagat ttttcaggcg
      301 gtggatgcac agctcgatgc cgccggatga gcgggggatg acatctgcgc acgggcagct
      361 gacctccgcg ctatcgcctt cgcaggtccg cactgacccc gacagcctgc acgagtgggg
      421 gcgggactgg acccggactg ccgagcctgc gcctgccgcc gtcgtttttc ccgagactac
      481 cgctgacgtg gtcgccatcg tccgcctggc caactccgag ggcctgaagc tggtgccgtc
      541 gggtgggcgc acgggtcttt ccgggggtgc ggtggccgcg gacggagagg tggtggtttc
      601 cttcgaccgg atgaaccggg tgctcgagtt cagcgccgca gaccgcctgc tcagctgtca
      661 ggccggcgtc gtcacgcgga ccgtccagga ctttgcccgg gagcgtgggc tgtcttaccc
      721 ggtggatttc gcgtcctccg ggtcgagcca gatcggcggc aacattgcga ccaacgcggg
      781 gggcatcaag gtgatccgct acgggctcac ccgggactgg atagccgggc tcagggtcgt
      841 gacgggcgcc ggcgagctgt tggactgcaa tcgcggattg gtcaagaacg cgaccggata
      901 cgatctcaga cacctgttcg tgggctccga gggcacgctg ggtttcatcg tggaggcgga
      961 tgtccggctg gctcccgctc cggctccgca aacggtcatg ctgctcggcg tgaccgagtt
     1021 cccgaggctg atcgacgtgc tgtccgtgct gcagagcacc gtcgtgctct ccgcgttcga
     1081 gttcttctcc gaacttgcgc tgcagaaggt tctctcgcat cggaagctca ggcgacccct
     1141 ggccgagacg gcgcccttct atgcgctggt ggagttcgac acggcggact tagagcccgc
     1201 gacggcggcc tatgaggccg cggtgggcgg cggctgggtg tgtgacggcg tcgtcagcca
     1261 gtccgagggc caggcggcgg cgctgtggtc gctcagggag ggcatctcgg agagcatcgc
     1321 gcagttcacg ccttacaaga acgacctgag cgttcgcgtc tcggtgttgc cggagttcct
     1381 cgctcaggtc gacggcctgg ttgcccagag ctatcccggc ttcgaggtgt gctggtacgg
     1441 gcacatcggc gacggcaacc tccatctcaa catcctcaag cccggcgggc tctcgcttgc
     1501 ggagttctac gctcgctgcc acgccatcag ccccagcctc ttcgagctcg tggcccgcca
     1561 cggcggcagc gtatctgccg agcacggcgt tggcctgctg aaacgcgact tcatcggcta
     1621 ctcccgcagc gcggccgaga tagagatcat gcgcgcgctc aagggcgtgc tcgatccgaa
     1681 tcagatcatg aacccgggca agctcctctc ctgaggctca tgcgccgcag ggcgcggatc
     1741 ccggctcctc agaacgacgt cacgccggat gcgccggcga cgatgaccag gtcggcggcc
     1801 tgggcggcaa acacgccgtt gcataccacg ccgacgatgt tgttcacggc cgactccatg
     1861 gcggccgggt cggtgatggt cagctcgtac acgtcgagga tgatgttgcc gttgtcggtg
     1921 acgaaaccct cgcgcagctc gggagcgccg ccgaggccga cgatggcgcg cgcggccaga
     1981 cctctcgcca tggggatgac ctccacgggc agagggaacg cgcccagtgt ctgaacgacc
     2041 tttgattcgt cgacgatgca aacgaactcg tcggcggacg ccgccacgat cttttccctg
     2101 gtgagcgccc cgccgccgcc cttgatgagc tgccttgccg cgttcacctc gtcggcgccg
     2161 tccacgtaaa ccgccacggc cgccgcagcg ttcagatcga tgaccggaat gccgacagcc
     2221 ttgagccggg ccgcgctcgc ttcgctgctg gcaacggcgg catcgaattt gtgcctgatg
     2281 gcgccgagcg cgtcgatgaa gtggttggtg gtggagccgg tacccacgcc gacgatgctc
     2341 gcgctgccga gcttgggctc gatgtaggcc agcgctgcct ctgcggcact gcgcttgagc
     2401 tgatcttggt ccatacttac gtccccgaag ccgtgctcga tgcgcggcgc ccacaaaccg
     2461 ctgcgaccct aactcatacg attgccgtgg aaaagtacgt taaaaagatt ctctcctcgc
     2521 gtgtgtacga agcggccgta gagaccccgc tgcagcccgc gcgcaatctg tcgatccgcc
     2581 tgggtaacca cgtgcggctc aagcgggaag acatgcagcc cgtcttctcg ttcaagattc
     2641 gcggtgccta caacaggatc gttcagctca ccgaggagga gcggggccgg ggagtcatcg
     2701 cggcgtccgc cggcaatcac gcccagggcg tcgcctactc tgcccagcag ctcggaatca
     2761 aggcgctcat agtgatgggg cgcaacacgc cgagcatcaa ggtgaacgcg gtgcgcgccc
     2821 tgggcgccaa ggtcgtgctg cacggggacg cctacgacga ggcggccgcc cacgcgttga
     2881 agctggccga gcgcaacggc tacgtgttcg tccatcccta cgacgacccc gacgtcatcg
     2941 cgggtcaggg caccgtgggc gtggagattc tcaaccagca cgtcgccgtg ccggacgcca
     3001 tcttcgttcc ggtgggcggc ggcgggctga tcgccggtat cgccgcctac gtcaagtacc
     3061 tcgcgcccga tacgcgcatc atcggtgttg aggcggaagg ctcggcgtgc ctggcggcgg
     3121 ccatggcggc cggtcggcgg gtgaggctgc ccttcgagag ccttgatctt ttcgccgacg
     3181 gcgtgtctgt ggctcagatc ggcaaggaaa ccttcaaggt ggccaggcgg tgcgtcgatg
     3241 aagtcatcac cgccaacaca gacgagatct gcgccgcgat aaaagacctc ttcgacgata
     3301 cccgctcaat cgcggagccg gcgggcgccc tggcggtggc cgggctgaag aagttcgtcg
     3361 agcgggaggg cgttcggaac ctgaacctgg ttgccgtggt cagcggcgcc aacgtgaact
     3421 tcgaccggct gcggcacatc tccgagcggg cggaggtggg ggagcaccgc gaagtcgtcc
     3481 tgggcgtcac gatcccggag aagccgggca gcttccttaa gttctgcaag atgctcggca
     3541 accgccctgt cagcgagttc aactaccgat tcgccacgga ccgggaagca caggtctacg
     3601 tcggcctgca ggtatcggcg gccgacgata gggccgagct catgaaaagc ctctccacag
     3661 gctacgtcgt gcacgacatg acggacaacg aggctgcaaa gctgcacgtc cgacacatgg
     3721 tgggcgggcg caccttcggc agcaagccgg agctcctcta tcgattcgag tttcccgagc
     3781 ggccgggtgc gctgcttcgc tttctgaccg gcctggggtc tcgctggaac atcacgatgt
     3841 ttcattatcg taatcatggg gccgcatggg ggcgcgttct ggtgggcttc gaggctcagc
     3901 cacgcgaccg gcgctccctg ggcgagtacc tcaagcgcat cggctaccgc tactgggaag
     3961 agacggacaa cccggcctac cagctgtttc tgcgctaggg gccgttactc ggccagatag
     4021 cgctcgaact cctcgtcgaa tgccagcagg gctggatcct tgagatggtc cacgtacaac
     4081 cgaccgtcca gatggtcgaa ctcgtgctga aacaccgtgg cgaggaaccc tttcagctcc
     4141 agatcgagcg gtttgccctc ctggtcgagc gcgttcacgc gaacatgctg gggccgttcc
     4201 acgaatcctc gcagccccgg aaccgacagg cacccctccc agtagcccgc ggtggcagga
     4261 tccacgacct ccacggacgg gttgatgaac accgtcagcg gcagagcggg gatctcgccg
     4321 tagcgggtgg ggccgccggg aatctcgatg atggccagcc gcacggactc gttgatctgc
     4381 ggggccgcga gacctatgcc gccgtagtcg tgaagggtgt cgatcatgtc gccgatcaga
     4441 cggcccaccg cctcgctgcg gatctcttcg ccggccagcg gcctggcgac ccttcggagg
     4501 gtggggtgtc ccatgcggat gattttgcga acggccatgg gcggagacga tacggggtca
     4561 ggtgaacgct tgccagccgc gctcggtcac gatgccgtcc agagggatgt cccagtcctg
     4621 gaacggcagt gggtcggcgc ttcgctgcac ttcgtgggcg atgccgacga tccggggccg
     4681 aagcggttcg ggcagcgcgc ccaggaaccg gtcgtagtag cccgcaccca tgcccaggcg
     4741 aacaccgtac tcgtcgaagg cgaccagcgg gagaaggatc aggtccaggc ttctgccgtc
     4801 cacgaaagcc gagccggggc ccggctcggg aatcccgtag cggttcacca gcatgggcgc
     4861 ggccctatgg taacggtaaa aggccattct ccctgcggtg ctgactaccg gcagagcgag
     4921 ctggcggttt cggtactcca gctcgccgag caggggcgcg aggctcactt ctccatcgtt
     4981 ggcccagtac gcgccgattc gcctgctgcg ccatgacacg gggttgttga gcaggtgtcg
     5041 ggctacccgg agcgcgtgct ctcgctgctg atgagagccg agggcccgcc tgcgctcacg
     5101 gaagaaacgg cgaagttcct gacgttcgag ggtgctggtc atggcaggga accgggttgg
     5161 aaagagtctc ccgaaaatgc cgctgtttgg tttgccctga accgttgtgg ttcaaggcgg
     5221 ggacccgggc gacgtctaag gcttcccgat ctgcaccacc gtttggataa ccgcgggcgg
     5281 tatcggacag gctcaccggc gtcagcgccg aggtctccta tagtttatct aacggctcaa
     5341 ggacgacacc aactggcgaa cattccagga gacagcacgg agtatagccc aattgacgtc
     5401 gttgccacgg gttgacaacc ggtcacgatc gagaactgtg aaaccgttaa aggttcagct
     5461 gtttctgttc cgagagcgcg cggccgaccc gctcggtgag ctcggtcacc ttctggctga
     5521 cgacgccgtg agtggattcc aaagacttgg agctgttgat gaactcgttg gcgatgttca
     5581 gcgcggccat cacggcgatc cggtccatcc cgaaaatctt gccggactcg cgtatggaac
     5641 gcatctgctc gtcaaggtag cgggcagacc tcgagagcgc gtccacctct tcggcgggac
     5701 agctgacctg atactccttg tcgaggatct tcacgcttac ggtggtggct tcttcgctca
     5761 tgtcccctgc ttcgctaatc ctgctcgagc gccttcagtc gggtgatcat cgattcgacc
     5821 cgggctttgg ccagctcgtt tttctcaatg agcttcgccc gctccgtggt ccagttctgc
     5881 tgttgtgctc gcagcgcccg attctccttg gtcagcacgc tgcatagctc tatgagctca
     5941 tccactttgc tctcgagcgc cgtccattct gtgtcggcca tgccaggctt cacgcgtatt
     6001 ctgcttatcg caggatagtg tcgcctgaat gggctggcat gtgaagcatg taacttctgc
     6061 tagcattagg cctatcccgg ggcacgaagc tcgataggcg acatggcgga cctgacggcg
     6121 ctcgcacttg gtgcggtgga atctctttcg gttgccgaga tccacggcgc cgtgtgcggg
     6181 ctggcagtcg cctccgaggg gggaatcgag ctgggctctc tcgtcgagct ggtgggcatc
     6241 gaggcccttc gggacgaaca ctccgttgga gaatttgtta ccgccgccgt ggacgcgctc
     6301 cacgccgagg atctcagctt tgctccgctc ctcccggacg accatgtgga cctggcgctg
     6361 cgcctggaag cgctgggcga atggtgcggc gcgttcctgg ccggctttgc ttcaggatcc
     6421 cgatttcagg aactcggaga agagcagatt ccggaggagg cccgggaaat catcggcgac
     6481 ttcacctata tcgccaacgt tgacggctcg gaggccggcg acggggaaga tccggaatcc
     6541 gcggagaggg actatgccga ggtgcacgag tacgtgaagg tcggcgcgtt gcttctgcgc
     6601 accctcctgg gtggcgacgc gcctgaagag acggcgcggc cgtgattgcc gctgcggagt
     6661 accggcgccg ccgtcagcgc ctcatgagcc tgatggacga agggtccatc gccgttctgc
     6721 cggcggccgg ccagcgtttc cgtaaccgca acacggagta tccgttccgc caggacagtg
     6781 acttctacta tctgacgggt tttgcggagc ccgacgccgt tctggtgctg gcgcccgggc
     6841 gtgcgcacgg tcaggtcatc ctcttctgca gagagcgcga ggcgcgcgcc gagcgctacg
     6901 acggcgagcg ggtagggccc gagcgcgccg cggagcgcat tggcgtggac gacgctttcc
     6961 ccgtcaacga cctggaggag attctgcccg gcatgctcga gggacgggag cggatctaca
     7021 tcaccctggg cgaatacccg gagttcgaca accgcctgat gcactgggtc gccggcatcc
     7081 gcgcgcggga agcgggcggg gccattccgc cgggtgagtt cgtcgcgctc aagcatctgc
     7141 tgcacgagca gcgtctctac aaatcggccg ccgagctcag ggcgatgcgg gaggcggccc
     7201 gtatcacctg ctccgcccat cgccgcgcca tggcggcctg tcggcccgga atgaccgaga
     7261 cgcagctgga agccgagctg ctgcacgagt tcatggtcaa cggtgctcgc agcccggcct
     7321 atccgagcat cgtgggggcc ggcgcgaacg cctgcgttct gcactacgtg gacaaccacg
     7381 acgtgctgaa gaagggcgat ctcgttctca tcgacgccgg ctgcgagtat cagcactacg
     7441 cctcggacgt aacccgcacg tttcccgttg ccggtaagtt ttctgctgcc cagcgggctc
     7501 tctacgaggt cgtcctcgaa gccaatcgtc agggcatcga ggcctgtcgc cccggtgcga
     7561 cgttcatcga gcctcactct acggcgctca aggtcatggt ggaggggctc atcgagctga
     7621 atctcataca aggcgatgtg gaggagattc tccagagcga acgctatcgt gaattctgtc
     7681 cccacaactc gtctcactgg ctgggcagcg acgtccacga cgtgggcgac taccgggtcg
     7741 aaggtgcctg gcggccgctg gagccgggca tggtgctcac cgtggagccc ggcatctaca
     7801 tcccggccga cgactctacc cgacacttgg cgccgcggtg gcgcggcgtg ggcattcgtg
     7861 tggaagacga cgtggccatc accaggcagg gccatgaagt gctttcggcg tcggccccca
     7921 agtcggtgga cgacatcgag cggctgatgc gtgggggcgc cgatggctga cgtggtcgtg
     7981 gtgggcggtg ggctgatcgg agcgctggcc gccctcggcg tcgccgatgg gggacgacgg
     8041 gtcacggtgc tcgaacggcg cgagccggaa aggcagcggg gcaggctggg cgtcgacatc
     8101 cgcaacgtgg ccgtatcgcc agcgtcccgc gagcttctgg aagccgtcgg tgtgtggtca
     8161 tcgctcgagc cggctgctta ccaacgcatg gaggtatggg aagagcgagg cactcgcggc
     8221 atgcacttcg acgcggcaga ggtaggccgg gccgagctcg gctggatcct ggaatcgagc
     8281 caggtcgtgc gctcgctctg ggatctgctg cagcggcacc ggaacgtccg gctggaggtc
     8341 ggcgcggatc tggcgagctt gcgcctggcg gcttcgggcg ccgtcttgga aaccgaccgc
     8401 ggagatatcc gggcgcgcct ggtggtggcc gctgacggtg cacgttccag ggtgcgggag
     8461 ctgctcgcgg tgcccgccga agaagctccc accggtcatc atgccctcgc cacggtggtg
     8521 cgcaccgagc aagagcatga gggcacctgc ctgcagcgat tcctgctcga gggcccgctt
     8581 gcgctgctgc cgacggcgga cgcccacctt tcatccgtgg tctggtcgca gcccgcggag
     8641 gaggccgagc ggcgtgcgaa agagtccgac ggcgacttct gcctcgagct ggagcggtgt
     8701 ctggaagcac gatttggaaa ggtggaagcg gtggatgccc gggtcgtgtt tcccctggcg
     8761 cagacgctgg tgtcgacctt caatccccac cacagggtgc tgctgattgg cgacgccgcc
     8821 agggtcgtgc accccctcgc gggcatgggc gcgaacatcg gcttcgagga cgtgcgcgat
     8881 ctgctggccg aactcgccgg gataggcggc gatcaggacc cgggagcgcc gggaatctgg
     8941 cggcgctttg cgcggcagcg acggctacga tcccagatgg tgctggcgct gatgtccggc
     9001 ttccggcgcg cctattctgc ggacggcccg ttggccagct gggttcggaa cacgggcgtt
     9061 ggatggctcg accgtgcaca gcctttgaaa gcacagatca tgagagaggc tttgggggtc
     9121 gggccgctcg ccagaaggtg gtgacgccca ccgtttccgg ggccacccgg ttcgcgtcat
     9181 cccatgcgtc atcgcataca atgtggtgcg tttttccaat ggtggtctga gcatgacgcg
     9241 gcgaaccccg ctatatgagc tgcatgtcgg gctgggcgcg aagatggtcg acttcgccgg
     9301 ctgggaaatg ccgctccatt acggatccca gctggatgag catcacgccg tgcgtcggca
     9361 cgttggcctg ttcgacgtct cccacatgac catcgtggat gtcagcggtg cccgcagtct
     9421 cgacttcctc cagcggctgg tggccaacga cgttgccagg ctggaagcgc ccggtcaggc
     9481 gctgtacggg gtgctgctca atccggcagg cggggtgatc gacgacctca tcgtctatcg
     9541 ccgcgatgcc gacttccggc tagtggtcaa tgccgccact cgagaaaagg tgctcaactg
     9601 gctcgcgggc agcaacgagg aagcggtcaa cattctggag cgggacctgg ccatgctggc
     9661 catccagggc cccgcggccg tggagatgta tcagtccctg cctgacagcc tcgaggtccg
     9721 gggcttgaag gcgtttaccg cggccgtgcg cggggaggag atgattgccc gaacggggta
     9781 cacgggggag gacggtctgg agatcatggc cccgggcgat cgcgcccgtc agctttggct
     9841 tgagctcatc gggtccgggg ctcagccagc ggggcttggg gctcgcgaca ctctgcgcct
     9901 ggaggctggc ctgaacctct acggccagga catggacgat gaaacctcgc ccctggtgtc
     9961 caatctggcc tggaccatcg cctgggagcc atccggacgg aacttcatcg gccgccaggc
    10021 cctcgaggac gaacgcgcac gcgggatcgc ggagaggctc accggtatcg tgctcgagga
    10081 caagggggtg attcggcggg ggtgccgcgt agtgaccgcg gccggtgatg ggcacgtcac
    10141 cagtggtata ttctccccga cgttgggata cagtattggg ctcgcgcggg tgccgaaagg
    10201 cgcgaagggg ccgtgcgagg tagaaatgcg gggaaggctg aaacaggcgc gaatcgtccg
    10261 gcctcccttt gtgcgtaatg gtttgcaggt attcgaatga gcgacgttcc acaggagctt
    10321 aaatatgcgc gcacccacga gtgggcgcgg ctggaggagg acggcaccat caccgtcggg
    10381 atttccgacc acgcccagga tgcgcttggc gatgtggtct acgtggagca tccggagccg
    10441 ggtcagccgg ttgcggccgg cgacgaggcg ggcgtcgtcg agtcggtgaa ggcggcctcc
    10501 gacatctacg cgccggtaag cgggagcgtc attgccgtga acgaggcgct ggacgacgcc
    10561 ccggagaccg tgaaccagga tccctacggt gacggctggt tcttcaagat acagccggac
    10621 gacgttgccg agctggacga gctgctcgat tcggaagcct acgccgaact atgcgagaac
    10681 gaagagcact gagtccttga tacccatcta aacggagccc ccgatgccct tcataccgca
    10741 caccgcggaa gaagtgcagg tcatgctgtc ccagatcggc gtggcccgca cggaagacct
    10801 gttcgacgag attcctcaga atttgagagc aggcgcgctg agctcggtcc ccgcgggggc
    10861 cagcgagctg gagatgctgc gcaagctcgg cgcgcgcgcg gcgctcgacg agtcggtagt
    10921 ctgcttcgcc ggggcgggca gttacgacca tcacattccc gcggcggtct gggatctgac
    10981 gagccgcggc gagttcatga cggcgtacac gccctatcag gcggaagcga gccagggcac
    11041 gctgcagctg atctacgagt accagtcgat gatgaccgcg ctcacgggtc tggacgtctc
    11101 caacgcgtcg gtttacgacg gcgcgtcggg cctggcggag gccgtgctca tggcggtgcg
    11161 aagcaacaag aaggcccggt cgcgaaccgt tctgctgccg gactccctgc atccgcacta
    11221 cgcggccgcg gcccgcaaca tctgccggca tcagagcgtg gagttcgtcg gcatggccct
    11281 ggaccacgac ggccgcgtgg atctcgaggc cctggagacc gatggtccgg acgaggtctg
    11341 cgcggtggtg gtgcaacagc cgaacttctt cgggatgctc gaggacgtcg acgccttaac
    11401 ggattgggcg gccgagcggg ggggcctcgt gatcgcggtg gtcaacccgc tggctctggc
    11461 tgtgctgaag cctcccggtg agtggggggg cagcggggcc gacatcgcct gcggcgaagg
    11521 ccagccgctc ggcgtgccca tgtcttcggg ggggccggcg ttcggcttca tctgttgccg
    11581 ctcggccctg gtgcgtcagc ttccgggaag ggtgatcggt cggacggagg acctgacggg
    11641 gaagccgggc ttcactttga ccctgcaggc cagggagcag cacatccgcc gaggcaaggc
    11701 cacgtctaac atctgcacga accagggctt gctggtcacg gcggccacca ttcacatgag
    11761 cctcttgggg ccggtcgggc tcgccaggat tgccgagcat tgccacgcca atacccgcgc
    11821 tctggtggag gccctgaccg ccatcgacgg ggtggaggcg cggcatcagg ggccgttctt
    11881 tcacgagcgg gtgttgcagc tgcccaggcg ggccgacgag gtcgtagccg gcctgctcga
    11941 gcgcgggata ctcggcggcg tggcgctcgg cgcgtatcac gagagcatga atcacgacct
    12001 gctcgtctgc gccacagaaa agcggacgga cgacgagatc ggccgctatg cggccgccct
    12061 gagggaggtg atcgcccgat gagcgatgtg cgaacgatat tcgagctcag cgcgcagaac
    12121 cggggtgccg ccgctcagca cttgagcgag cggccgggag ccatggacga cattcccgcg
    12181 agcctgcgtc gtgagcgggc tcccggcctg cccgaagtat cggagcttca ggtggtgcgg
    12241 cactacacca acctgtcgcg caagaatttc tccatcgata ccaatttcta tccgctcggc
    12301 tcatgcacca tgaagtacaa cccgcggggc gcgcatcgcg ctgccagcct gccgggtttc
    12361 ctgaaccggc acccgcttgc cccggactcg gccagtcagg ggttcatggc ctgcctgcac
    12421 gagcttcagg agatcctcaa ggacgtcacg ggcatgcagg gagtgtccct cacccccatg
    12481 gccggggcac aaggcgaatt cgccggcgtg gccatgatcc gcgcctatca cgaggcccgg
    12541 ggcgatcatg caagaaacga aatcctggtg cccagcgccg cccacggcac caacccggcg
    12601 acggcggcga tgtgcggcta ccgggtagcg gaagtgcccg tcaacgcctc gggcgacgtc
    12661 gacctcgaag ccctcaagct tgcggtagga ccccagacgg cggggctgat gatgaccaac
    12721 ccgtccacct gcggggtctt tgagcgtggc atcgagagca tcgcggcggt ggtgcacgat
    12781 gccggcggcc tgctctacta cgacggagcc aacctgaacg ccattctcgg caaggtgaag
    12841 ccggggtcca tgggcttcga cgtgcttcac atgaacctgc acaagacgtt cgcgaccccc
    12901 cacggcggcg gcgggccggg cgcgggcccg gtgggcgtcg gcgaacggct gctgccctac
    12961 ctgccggtgc cgttcgtcgc ccaggatgca aaaggcgcat accggtgggt aaccgacgcc
    13021 gagctgcccg acagcatcgg ccgtctgtcg gcctacgccg gcaatgctgg aattctgctg
    13081 cgcgccctcg cttacgcgct gatgctcggg cgggaaggca tgcaccgggt gggcgagtac
    13141 tccacgctca atgccaacta cctggcgcga cgtcttgaag atgcaggctt cgagctggcc
    13201 tatccggacc ggcgggcgac ccatgagttc atcttgacct tttcggagtc ggcacgcagc
    13261 cgggacgtga cggccatgga tttcgccaag cgactcctcg agtacggcat tcactctccg
    13321 accacctatt tcccgctgct gatacccgag tgctttctga tcgaacccac cgagacggag
    13381 accaaggcgg agctggacgc gttcgtggat gccctggtgg cgattcagcg cgaagccgaa
    13441 gccgatcccg aaaaagtcaa aggcgcgcct tacgatctgc ccgtcaggcg gttggatgac
    13501 gtcaaggccg ctcgcgaact ggacctggcc tggcgcggct agacacgggg ccgggatctc
    13561 aacgcttcgc cgagatcctc cggcgtgccg atgggcagcg agcaggacag cccgctgcac
    13621 acgtatgcca ccgtctggct gtgcggatcg tcgctcacgg gtccgggaag gtaggcgggc
    13681 agcgtgctga cgccctcaaa gggtatgcag tagacggccc gccatggcgt gtatcccgac
    13741 accgcggccc ggcgccagtc gggaaacgtc gcggcggcac cgcggacgac gacgagctcc
    13801 gggggcatga tctcgtcttg caggcccgag agcagacagc agtggccggc cggatactgc
    13861 tccatggccg cgcgcgccca cgccagagtg gccgccgcag cctcgaggta gcgcgtctct
    13921 ccgaaaaggt ggcccagcgc cgtgagcgcc tgaaccatca cgccgttgcc cggcggcagc
    13981 gcgtcgtcca tggtgggctt tggccgatga atcagggtct cgtgctggtg ggcggtaaag
    14041 aaaaagccgc ccctgtcggc atcgtagaac cgctccaggg caccgtcagc cagcttgcag
    14101 gcgaaggcgg cgtcctcttc gcgccaccgc gccccaagaa gggcaatcag gccgtcgagc
    14161 aggttggcgt agtcgtccag gtaggccgga tagcggggcg ccccctgcca ggtcgcgtag
    14221 agcatgccgt cccgccagag ccgggcgcgc gcgaaatcgg ccgcctgagc ggccgcatcg
    14281 agccatcgcg actgccccag gcgtgacccg gccctggcca ggcccttgat ggccaggccg
    14341 ttccaggcgg tgagcacctt gtcgtcgagg ccggggcgaa cccgcagctc ccgggctgcc
    14401 agcatcttgc ggcgcgcgct ggccagcagg tcgttcgcag cggcgcgatc catggacagt
    14461 cgctcgacca ccgagcgcca ggcatcgtat cggtgcagat tccacttgcc ctcgaagttg
    14521 gccggtttgt ccaggccgta gagcgtttcg acgaccagat actcgtcctc gtcgagcagc
    14581 cgtttcacct ggtcgcgccg ccacaggtag aacttcccct cctcgccctc ggagtccgcg
    14641 tccagcgcgg cgtagaagcc gccctggggg tgacgcatct cgcgcaggag ccagtcggcg
    14701 gtatcgctca ccgcctggga gaagagctcg tcgggaccaa tggcaagcgc gtcgctgtag
    14761 agagccagga gttggccatt gtcgtaaagc atcttctcga agtgaggaat catccagcga
    14821 cggtccgtgg cgtagcggca gaagccgccg cccaggtggt cgtatatccc gcctcgcgcc
    14881 atgcgcgtca gggtggtcat gacgaactct agcccctgcc ggtccgcctc gcccgacctc
    14941 tgggcgtagg cccagtggcg cagcaggtag gtcacggtct ccggcatggg aaatttgggt
    15001 gccccgccga acccgccctc atcctcgtcg gcctgcgcgc cgagctgctc tctggcagcc
    15061 gccagcagct cggcgtcacc gagctcgccg gcctgtgaat ctccgcctgc cagccggccg
    15121 agcacgtcgg cgacccgcgt gccgtgatct ctcagctcgt cgcgctggct ggcaaacgtc
    15181 tcggcgatcc tcagcagaag atcgacgaat ccgggcagct gatagcgggg cgttttcggg
    15241 aaataggtgc cgccgaagaa tggcagcctc gtgtcggggt ccagaaacat ggtcagcggc
    15301 cagccgccac cctgttgggt gaggagctga tgggcgagct ggtagacctt gtccagatcg
    15361 ggcctctcct cccggtccac cttgatgttg acgaagtgct cgttcatgac ccgggcagtg
    15421 gccgggtcct cgaaggattc gtgggccatc acgtggcacc agtgacaggc cgagtagccg
    15481 atggagagca ggatgggttt ctgctgctcg ctggccaacg cgagcgcggc ttcatcccag
    15541 gggtgccaat ctaccgggtt atcggcgtgt tgccgcaggt aggggcttgt ctcgtcggcc
    15601 aggcggttgc tcaaggttcg aggctctttt cggctgtgca gggtcagcat cctgacacgg
    15661 aacacgaccg ggtcaactcg gtcggcccgt cggggccgca gttggtaatt cggcccatat
    15721 tgaggttcaa tcgagggaaa ttcagaggaa tgccgatggt tgatgcggat ggtttccgcc
    15781 caaacgtcgg aatcgttctg tcgaacagca ttggccaggt gctttgggcc cgccgggttg
    15841 gcggccagga cgcctggcag tttccgcaag gcggcatccg tgagaacgag tcagtacaag
    15901 ccgcgctttt tcgcgagctg ttcgaggaag tgggcctcgt ggcggacgac gtcgaggtgc
    15961 tggcaagcac ccgcggctgg ctccgctacc ggctgccgcg caggatgcgt cgttacaact
    16021 ccagccccgg tttccagggg cagaagcaga agtggtttct gcttcgaatg ctgggcgacg
    16081 acgcgcgcgt gcagatggac gggagcgcca agcccgagtt cgattcctgg cgtt
//
LOCUS       DBNHOX010000383         2656 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_801214, whole genome shotgun sequence.
ACCESSION   DBNHOX010000383 DBNHOX010000000
VERSION     DBNHOX010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_801214"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..2581)
                     /gene="ppsA"
                     /locus_tag="AC16UZ_08540"
     CDS             complement(152..2581)
                     /gene="ppsA"
                     /locus_tag="AC16UZ_08540"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HIR6246769.1"
                     /translation="MAAAPGYVRWFSEIASADLALVGGKNASLGEMYQELTSEGLRVP
                     NGFALTTRLYRDALAEAGIDGALREVLDALDVNDVESLQLGGQRCRELVYDAGVPVGG
                     REQILGAFAELRAQYGADLRVAVRSSATAEDLPHASFAGQHDSYLNISGGEALIDACR
                     RCLASVFTDRAIRYRADHGFDHLEVALSVGIMKMVRADRGSSGVIFTLDTESGFRDVI
                     LINSSWGLGENIVQGTVDPDEFYVHKPTLRQGHRCVLRRKLGEKQMRMLSGSRRGGGS
                     TYNVPTDPADRARYSLSDEDVLALADQALIIEAHYSRRAGADVPMDIEWARDAEDGEL
                     YIVQARPETVASRANPNRLVHYRLSGSAPELARGRAVGSGVATGTARVIRDPREIRDF
                     VEGDVLVADTTAPDWGAVIKKASAIVTNRGGRTCHAAIVARELGIPAVVGSGTATEQI
                     PSGDVVTVSCAEGEVGRVLAGTVAFETETMDLESLPRTRTQMMLNVGNPDVAFKMAML
                     PNDGVGLARLEFIITEYVRAHPMALLHPERIQDPGIERELRELCRGYADPAEFFVARL
                     AEGVGTIAAAFYPKPVVVRLSDFKSNEYADLLGGRAFEPVEENPMLGFRGASRYAHPA
                     YEAGFALECAALKRVRETMGLHNVRVMVPFCRRVEEAARVLATMAAHGLARGENGLEV
                     YMMCEIPNNVLLIDEFSAYFDGFSIGSNDLTQLTLGVDRDSEMVAFEFDERDPGVLRM
                     LELAVAGAKRNGKHSGICGQAPSDYPEIAEFLVRRGIDSLSLNPDSVVAATELVAQVE
                     AKIAAEGADGA"
BASE COUNT          460 a          926 c          834 g          436 t
ORIGIN      
        1 ggataccgcg caacgctctg caatggtcag gtgatcctgg agaacgacga gcataccggc
       61 gcgcgcagcg gcgaggtgat ccgccgctga gccgggcggg caacctttac gctcgccgcc
      121 cagggcatcg cccgctccgg ggtcgacgcg ctcaggcgcc gtcggccccc tcggcggcaa
      181 tcttcgcctc cacctgggcg accagctcgg tggccgctac caccgaatcg ggattcaggc
      241 tcagcgaatc gatgccccgg cgcacgagga actcggcaat ctcgggatag tctgagggcg
      301 cctgcccgca gatgcccgag tgcttgccat tacgcttggc accggcaacc gcgagttcca
      361 gcatcctgag cacaccggga tcccgctcgt cgaactcgaa agccaccatc tccgagtccc
      421 ggtcgacgcc aagggtaagc tgagtcaggt cgttgctgcc gatggaaaag ccgtcgaaat
      481 acgcggagaa ctcgtcgatc agcaggacgt tgtttgggat ctcgcacatc atgtagacct
      541 ccagaccgtt ttcgccccgt gccaggccgt gggcggccat ggtggcgagc acccgcgctg
      601 cctcctcgac gcgccggcag aacggcacca tcacgcggac gttgtgaaga cccatggtct
      661 cgcggacccg tttcagcgca gcgcactcca gcgcaaaccc cgcctcgtag gcgggatggg
      721 cataccggga agcgccacgg aaacccagca tcgggttttc ctcgacgggt tcgaaggccc
      781 ggccacccag cagatcggcg tactcgttgc tcttgaagtc ggacaggcgc accacgaccg
      841 gtttcggata gaaagcggcg gcgatggttc cgaccccctc cgccaggcga gccacgaaaa
      901 actcggcggg gtcggcgtaa ccgcggcaca gctctctgag ctcccgctcg atacccggat
      961 cttggatgcg ttccgggtgc agcagcgcca tggggtgcgc gcggacgtac tcggtgatga
     1021 tgaactccag ccgggcaaga ccgacgccgt cgttcggcag catggccatc ttgaaggcga
     1081 cgtcgggatt tccgacgttg agcatcatct gggttctcgt cctgggcagg gattccaggt
     1141 ccatggtctc ggtctcgaag gcgaccgtac ctgccagaac acgtcccacc tcgccttccg
     1201 cgcaggaaac ggtcaccacg tcgccggatg ggatctgctc ggtagccgtt cccgacccca
     1261 ccacggcggg gatgcccagc tcccgggcaa cgatggccgc atgacacgtt cgcccgccac
     1321 gattggtgac gatggccgag gctttcttga tcaccgctcc ccaatccggc gcggtggtgt
     1381 ccgcgacgag cacgtcgccc tcgacaaagt cgcgtatctc ccgtggatcc cggatcacgc
     1441 gggcggtgcc ggttgccacc ccgctgccaa cggcccgccc gcgcgccagc tccggcgcag
     1501 agcccgacaa ccggtaatgc acaagccggt tcgggttcgc gcgcgaagcc acggtttccg
     1561 gtcttgcctg cacgatgtag agctcgccgt cctcggcgtc cctggcccac tcgatgtcca
     1621 tgggcacgtc cgcacccgca cggcgcgagt aatgcgcttc gatgatcagg gcctggtcgg
     1681 caagggcgag cacgtcctcg tcggagagcg agtaccgcgc gcgatcggcc ggatccgtcg
     1741 gcacgttgta ggtcgatccc ccaccccgcc ggctccccga caacatgcgc atctgcttct
     1801 cgccgagctt ccggcgcagg acgcatcggt gcccctggcg cagggtgggc ttgtggacat
     1861 agaactcgtc gggatcgacg gttccctgga cgatgttctc gcccaggccc caggaggaat
     1921 tgatgagtat gacgtcgcga aaccccgact cggtatccag ggtgaagatc accccgctgc
     1981 tgccccgatc ggcgcggacc atcttcatga tccctacgga cagggccacc tccagatgat
     2041 cgaagccgtg gtctgcccgg taccggatgg cgcgatcggt gaaaacgctg gccagacagc
     2101 gccgacaggc gtcgataagc gcttcgcccc ccgagatgtt gaggtaggaa tcatgctgac
     2161 cggcgaagct ggcgtggggt aggtcctcgg cggtggcgga gcttctcacc gccacccgca
     2221 ggtccgcccc gtactgagct ctcagctcgg cgaacgcgcc gagaatctgc tcccggcccc
     2281 ccaccggcac cccggcgtcg tagacgagct cgcggcagcg ctgaccgccg agctgcagcg
     2341 attccacgtc gttgacgtcc agggcgtcga gcacctcgcg cagagcgccg tcgatcccgg
     2401 cctcggcaag cgcgtcccga tacagcctcg tggtcagggc aaacccgttg ggcacacgaa
     2461 gcccctcgct ggtgagctcc tgatacatct cgccgagcga cgcgttcttg ccgcccacca
     2521 gggcaagatc ggccgaggcg atttccgaga accatcgaac ataaccgggc gctgccgcca
     2581 tgagccctcc ctcgcattgc cgtttctgct ccctcaggcc ccgacgttat ctaggggtaa
     2641 ttccgtaatc aatggc
//
LOCUS       DBNHOX010000384         2988 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_159136, whole genome shotgun sequence.
ACCESSION   DBNHOX010000384 DBNHOX010000000
VERSION     DBNHOX010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_159136"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="AC16UZ_08545"
     CDS             <1..579
                     /locus_tag="AC16UZ_08545"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR6246770.1"
                     /translation="QGTIYPDVIESAAGKYGKAHVIKSHHNVGGLPERMHLKLVEPIR
                     ELFKDEVRKIGMHLGLPRELVYRHPFPGPGLGVRVLGEVTAARADVLREADAIYLEEL
                     RAAGMYDAVSQAFAVLLPVKSVGVVGDARRYADVIALRAVETTDFMTARCARLPYELI
                     ERVSNRIINEIPHVSRVVYDVSSKPPATIEWE"
     gene            604..1050
                     /gene="tadA"
                     /locus_tag="AC16UZ_08550"
     CDS             604..1050
                     /gene="tadA"
                     /locus_tag="AC16UZ_08550"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017131735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR6246771.1"
                     /translation="MSHVDWMRLALEEAGRAAAAGEVPVGAVVVRDGELLGSGFNCPI
                     SSSDPTAHAEIVALRAAAKRSGNYRLAGAAMYVTVEPCTMCAGALVHARIATLVYGAL
                     EPRSGAVTSTATVLENPLLNHRVEVVGGVLARECAEVLRAFFAERR"
     gene            1078..>2988
                     /gene="purL"
                     /locus_tag="AC16UZ_08555"
                     /gene_synonym="purI"
     CDS             1078..>2988
                     /gene="purL"
                     /locus_tag="AC16UZ_08555"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIR6246772.1"
                     /translation="MLTLEGPPARSSFRLNKLSGQLGLPEGALYAQYVHLLAVHQPLA
                     GQALERAQALLRYGPETGLPAPTGDLVATVVPRAGTISPWSSKATDIFAICGLDQVTR
                     VERGVRWFADRGLSDVQLALLYDRMTERAVGEADFPSVFANLPPKPMSTVALQAGGLA
                     ALEEANRALGLALSADEIEYLASAYLELGRDPNDVELMMFAQANSEHCRHKIFNASWS
                     IAGEPQPLSLFEMIRNTYRSINGAGILSAYSDNAAVIEGHRMERFGVDSASHEYGYDE
                     AAVHVLMKVETHNHPTAIAPYPGAATGSGGEIRDEGAVGRGSKPKAGLTGFTTSHLNI
                     PGYGQPWELETGKPEHMVSALDIMVEGPVGAAAFNNEFGRPALAGYFRTFEQTVAGDP
                     RQVRGYHKPVMIAGGVGSIRPEHVQPTAFPAATALIVLGGPAMLIGLGGGAASSMGSG
                     ESSSDLDFASVQRDNAEIERRCQEVIDRCCALGERNPILLIHDVGAGGLSNALPELVK
                     DAGVGGHFQLRDVPNADPGMTPLEIWCNEAQERYVLAIAEEHLPGFAEICHRERCPFA
                     VVGRAVADPHLTVDDAEFANSPVDLPLSVLFGKPPKMQRSFAPRALTPPGFSHNGLVL
                     AEAIDRVLRFPCV"
BASE COUNT          478 a          943 c         1036 g          531 t
ORIGIN      
        1 cagggcacca tttatcccga cgtcatcgag tcggcagcgg gcaagtacgg caaggctcat
       61 gtcatcaagt cccatcacaa cgtcggcggc ctccccgagc ggatgcatct gaagctggtg
      121 gagccgattc gcgagctgtt caaggacgag gtccgcaaga tcgggatgca tctggggctg
      181 cctcgggagc tggtctaccg gcacccgttt cccggtccgg gcctgggcgt gcgggtgctc
      241 ggcgaggtga ccgccgcccg tgccgacgtc ctgcgcgaag cggacgccat ctacctcgaa
      301 gagctccgag ctgcgggaat gtacgacgcc gtcagccagg cgtttgccgt gctgctgccg
      361 gtgaagtcgg tgggcgtggt aggcgacgca cggcgttacg cggacgtcat cgccctgagg
      421 gcggtggaga ccacggactt catgaccgcc cgctgtgcac ggttgcccta cgagctgatc
      481 gagcgtgtat ccaaccggat catcaatgag atcccccacg tttcgcgggt ggtttacgac
      541 gtctccagca aaccgccagc gaccatcgag tgggagtgac cgtgtggttg gcacgggagg
      601 gcgatgagcc acgttgactg gatgcgcctg gctctagaag aggcgggtcg cgcagccgcc
      661 gcgggagagg tgcccgtggg cgcggtggtg gttcgggacg gggagctgct ggggagtggc
      721 ttcaactgtc ccatcagcag cagcgacccc acggcacatg cggagatcgt tgcgctgcgt
      781 gctgccgcta aacggtccgg caactaccgg cttgcgggtg ctgcgatgta cgtgacggtg
      841 gaaccgtgca ccatgtgtgc cggagccctc gtccacgcgc ggatcgcgac cctggtctac
      901 ggcgcgttgg agccgcgatc cggtgcggtg accagcacgg cgaccgttct ggaaaacccg
      961 ttgctgaatc accgggtcga ggtggtgggc ggcgtgcttg cgcgcgagtg cgcggaggtt
     1021 ttgcgcgcgt tctttgccga gcggcggtga gcatcggatg aggatcggtt catggagatt
     1081 ttgacgctcg aagggccgcc ggcccgctcc tcctttcgcc tgaacaagct gagcggacag
     1141 ctgggcctgc cggaaggtgc gctgtacgct cagtacgtcc atctcctggc cgtccatcag
     1201 ccgcttgcgg gacaggcgct ggagcgcgcg caggcgctgc ttcgctacgg tccggaaacg
     1261 gggctgccgg cgccgacggg tgatctggtc gcaaccgtgg ttcctcgggc cggcacgatc
     1321 tcgccgtggt ccagcaaggc gaccgacatc ttcgccatct gcggtctgga ccaggtgacc
     1381 cgggtcgaac ggggcgtgcg gtggtttgcg gacaggggcc tgagcgatgt ccagcttgcg
     1441 ctgctttacg accgcatgac cgagcgggcg gttggcgagg cggacttccc ctcggttttc
     1501 gcgaacctgc cgccaaagcc catgagcacg gttgctctgc aggcgggcgg cctggcggcg
     1561 ctggaagagg ccaaccgggc gttgggcctt gcgctgtccg cggatgagat cgaatacctc
     1621 gcgagcgcct atctggagct cgggcgtgac ccgaacgacg tcgaattgat gatgttcgct
     1681 caggcgaact cggagcactg ccgccacaag atattcaacg cgtcgtggag catcgccggc
     1741 gaacctcagc cgctttcgct gttcgagatg atcaggaata cctaccggtc gatcaacggg
     1801 gccgggattc tcagcgccta ttccgacaac gcagccgtca tcgaggggca tcgcatggag
     1861 cgtttcggtg tggactccgc gtctcacgag tacggctacg acgaagcagc cgtccacgtg
     1921 ttgatgaagg tagagaccca caatcacccg acggccatcg ccccgtaccc gggggccgcc
     1981 accgggtccg gcggcgagat ccgcgatgag ggcgccgtgg gccgagggtc gaagccgaag
     2041 gcgggcctta cgggcttcac gacctcgcac ctcaacatcc ccggctatgg ccagccctgg
     2101 gagctcgaga ccggcaaacc ggaacacatg gtgagcgcgc tggacatcat ggtggaaggc
     2161 cccgtgggtg cggccgcctt caacaacgag ttcggaagac cggctctggc cggctatttc
     2221 cgtactttcg agcagaccgt tgccggcgac ccccgccagg ttcgcggcta tcacaagccc
     2281 gtcatgatcg ccggcggcgt cggcagcatc cgcccggagc acgtccaacc caccgctttc
     2341 ccggcggcga ccgcgctgat cgtgctcggt ggcccggcca tgctcatcgg gctcggcggg
     2401 ggcgccgcct cgagcatggg atcgggagag agctcgtccg acctcgattt tgcctcggtt
     2461 cagcgggaca acgcggagat cgagcgccgc tgccaggagg tgatcgatcg ctgctgcgct
     2521 ttaggagagc gaaatcccat tctcctcatt cacgatgtcg gcgccggcgg gttgtccaat
     2581 gcccttccgg agctggtcaa ggacgcgggc gtcggcgggc attttcagct gcgtgacgtg
     2641 cccaacgcgg acccgggcat gacgcctctg gaaatctggt gcaacgaggc acaggaacgc
     2701 tacgtgctgg cgattgccga agagcatctg ccggggttcg ccgagatctg ccatcgcgag
     2761 cgttgcccct ttgccgtcgt gggccgggcc gtggccgacc cgcacctgac ggtcgatgat
     2821 gccgagttcg ccaactcgcc cgtggacctg ccgctttccg tgctgttcgg caagccgcct
     2881 aagatgcagc gttcgttcgc accgcgggcg ctcaccccgc ctgggttcag ccacaatggt
     2941 ctcgttctcg cagaggccat cgaccgggtt ttgcgctttc cgtgtgtg
//
LOCUS       DBNHOX010000385         5757 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_159357, whole genome shotgun sequence.
ACCESSION   DBNHOX010000385 DBNHOX010000000
VERSION     DBNHOX010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_159357"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="AC16UZ_08560"
     CDS             <1..420
                     /locus_tag="AC16UZ_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6246773.1"
                     /translation="LMEGPAHQVLSGALAFGEETAQYTVVHVVEPQYVQYSFDPTFTG
                     SLTRELEQQAIENATDRMSELCASMEMTPQEQQIVLGRAADQIHELAKHRDADLIVIG
                     SHGRHGWRRLLGSTANAVLHGAPVDVAVIRIPENQDT"
     gene            417..869
                     /locus_tag="AC16UZ_08565"
     CDS             417..869
                     /locus_tag="AC16UZ_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246774.1"
                     /translation="MTDNNSEGRISAFAKDVQRVAEEVEHLGEDTKLVGEEVVGAEIL
                     LTKELTEKIGIAWETAERAGAIGADQVRHLIEDYIATAREFSETPGLHNFLDLTLNHL
                     GRRITHVSEGARAVADLVAEESGRLEDAVLGVWKPFLAVLNRDWESRG"
     gene            919..2343
                     /locus_tag="AC16UZ_08570"
     CDS             919..2343
                     /locus_tag="AC16UZ_08570"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR6246775.1"
                     /translation="MKLLAIDKLQLPAFASTPDGRLVFVNAAWRERFAAGVGDPWHDP
                     FPKVEIDTIGEAWQACHLAHQPFMLEASSPSERYRILCQPVLENGQVLVLGTMADITQ
                     QSRQEAETEAILDTAVDAIIIIDETGRIETFNKAATQLFGYTAAETLGKPVSMLMPEP
                     HRSQHDHYMSRYLTTGEAKIIGIGRDLEAVDQSGRRIPIHLSVSEVQSESGRRFTGII
                     RDLSEQQAARQALAEQREKLAHVGRLSSMGEMTASIAHEINQPLTAISMYAQAGIKLI
                     DRGEPDLARLRDALEKLNTQSLRAGAVIERIQRFARAQKSERIMLDVNHLLDDLGKLA
                     ESDARLYNVELELELAPGLPEVFGDPIQVQQVALNLIRNAIDAMRDIRFVHGRRISLS
                     TSLRSDRMVEIAVSDLGPGVAEDQKELLFTPFHTTKKEGMGMGLSICRSIMTEHGGAL
                     SYRDNDGPGATFFFTLPTWRADDE"
     gene            2336..2959
                     /locus_tag="AC16UZ_08575"
     CDS             2336..2959
                     /locus_tag="AC16UZ_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6246776.1"
                     /translation="MSEHETTVFVVDDDEPVRDAIGLLLETVEIPYESFPTAQAFLDA
                     YDPSRTGCLVLDIRMPGMSGLELQERLLEDKAPIPIVFITGHGDIPMAVEAMKRGAID
                     FIRKPFRDQELLDRIHAALNADEDQRGAYDNLTTIRERVAALTPREHEVFERVAQGQA
                     NKVVAIELGISERTVEIHRSQVMQKTEARTLADLVRMKLTLDQADGP"
     gene            complement(3037..4077)
                     /locus_tag="AC16UZ_08580"
     CDS             complement(3037..4077)
                     /locus_tag="AC16UZ_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024779.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein N-acyltransferase Lnb
                     domain-containing protein"
                     /protein_id="HIR6246777.1"
                     /translation="MHSTGKQNFDDLDAEGRIGLVLRRGIRWTVVGFASLWAAGAVYV
                     DGPLGMDDGNIWLAVAWLAVAAACLALIRIAWKKYLVWLVWFLVVLIPWTAKNPSNER
                     PWKPEWAKTGWVEFSDGALEFHNFRNFDYGADGSVVESWETRTVSIRNLRGMDYFHDA
                     FGGDLIAHPMLSFDFGADGYVALSVETRREVDEAYSKLGGLYKMFELQYLWGDERDFV
                     RVRTNVRDEPVYLYRTSFPREQALFVLLDSVRETNLLARQPRFYNVISANCTTSLLAQ
                     TVEFRNAPFDIRMLANGRFDELIYETGGILGDGLPFEELRERALINPVALYAHGDAAF
                     SQRIRADRVGFD"
     gene            4276..4626
                     /locus_tag="AC16UZ_08585"
     CDS             4276..4626
                     /locus_tag="AC16UZ_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6246778.1"
                     /translation="MNANVCVVDGDPAVRDSLATLMRLSGHEVSTFATGQEFLDAAEA
                     DTVGCVICEADLPDTTGLAVFGALTEKSLAAPFALLISQSDPARVAQAYRAGIRNVFP
                     KPLVHRRILSFVSR"
     gene            4673..5569
                     /locus_tag="AC16UZ_08590"
     CDS             4673..5569
                     /locus_tag="AC16UZ_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6246779.1"
                     /translation="MELKNILVALHDRESMGSAMRKAARLEHFTGANITVVQTCWDVV
                     SEEPTQHFPQEEIDTIASRMKEAELNNLSAELARYSERVAELNAEILWSKHHDDAVAR
                     FAIQSGSDLIVAPRHARGGVKRLLLPEELKLAARAHAPLLLTSASTWREPTNVLVALD
                     AGDRQHAALNSLLIEWGRTLAETLGGELHLVAASPPPSSSAPNQTLEEFQREAEARRM
                     DALRALADAHPHDYGSLQVKTGDVCDVIDEVASACEVDIVVMGTAARTGLKRLFIGNS
                     AESVLSQLTEIDILTVPDSQQS"
BASE COUNT         1165 a         1877 c         1767 g          948 t
ORIGIN      
        1 ctcatggaag gtcctgccca tcaggtgctg tccggcgcgc tggcgttcgg agaggaaacc
       61 gctcaataca cggtcgttca cgtcgtcgag cctcaatacg tgcagtacag cttcgacccg
      121 acgttcaccg gatcactcac ccgcgagctc gagcagcagg ccatcgaaaa cgcaaccgat
      181 cggatgtccg agctgtgcgc ctccatggag atgacgcctc aggagcagca gatcgtgctg
      241 ggacgagccg cggatcagat ccacgagctg gcaaagcacc gcgatgccga tctcatcgtc
      301 atcggctctc acggaaggca cggctggcgc cgcctgctcg ggtccacggc caatgcggtg
      361 ctgcacggtg cgcccgtgga cgtggccgtc atccgcatcc cggagaatca agacacatga
      421 cggacaacaa cagtgaaggt cgcatatcag cgttcgcaaa ggacgtgcag cgtgtcgccg
      481 aggaggtgga acatctcggc gaggatacga aactggtcgg cgaggaagtc gttggcgcgg
      541 aaatcctgct gaccaaggag ctgaccgaga agatcggcat tgcctgggag acggccgagc
      601 gcgccggggc cattggcgcc gatcaggtcc ggcacctcat cgaggactac atcgcaaccg
      661 cccgggaatt ttcggaaact cccgggctcc acaacttcct ggatctcacc ctcaaccacc
      721 tgggaagacg gattacccat gtaagcgaag gcgccagggc cgtcgccgat cttgttgccg
      781 aggaatcggg acgcctggag gacgcggtgc tgggcgtgtg gaagccgttt ctcgcggtgc
      841 tcaatcggga ctgggagtcg cgcggctagg cggatccgac atgggcagca ccgcaaagct
      901 agccatgtcc gcggcaacgt gaagctgctg gcaatcgaca agctccagct tccggcgttc
      961 gcgagcacgc ccgatggccg cctggtgttc gtgaacgccg cgtggcggga acggttcgcc
     1021 gcgggcgtgg gagatccgtg gcacgatccg ttccccaagg ttgagatcga caccatcggc
     1081 gaagcgtggc aggcatgcca tcttgctcat cagcccttca tgctcgaggc cagctcgcct
     1141 tcggagcgct atcgcatcct ttgccagccg gtcctggaga acgggcaggt tctcgttctc
     1201 ggcaccatgg cggacattac ccagcagtcc cgccaggaag cggagacgga ggcgattctc
     1261 gacaccgccg tcgacgcgat catcatcatc gacgagacgg gccggataga gaccttcaac
     1321 aaggcggcca cgcagctgtt cggctacacg gccgctgaga ccctcggcaa gcccgtcagc
     1381 atgctgatgc ccgagcccca tcgatcgcag cacgatcact acatgagccg gtacctgacg
     1441 acgggcgagg cgaagatcat cggcatcggg cgtgatctgg aagccgtgga ccaaagcggc
     1501 aggcggatac ccattcacct ctcggtgtcg gaggtgcaat ctgagagcgg caggaggttc
     1561 accggcatca tccgcgatct ttccgagcag caggcggcgc gtcaggcgct cgccgagcag
     1621 cgcgaaaagc tggcgcacgt cggtcggctc agctccatgg gggagatgac ggcaagcatc
     1681 gcccatgaga tcaaccaacc gctcaccgcg atctcgatgt acgcccaggc aggcatcaag
     1741 ctcatcgacc ggggagagcc ggacctcgcc cggctgcgcg acgctctcga gaagctcaac
     1801 acccagtcct tgagagccgg agccgtcatc gaacgcattc aacggttcgc acgcgcgcag
     1861 aaaagcgagc ggatcatgct ggacgtcaat catctgctcg acgatctcgg caagcttgcc
     1921 gagagcgacg ccagactcta caacgtcgag ctggagctcg agcttgctcc cggtctgccc
     1981 gaagtcttcg gcgatccgat tcaggttcag caggtcgcct tgaacctcat ccggaacgcc
     2041 atcgacgcca tgcgggacat tcggttcgtt cacggccggc gcataagcct gagcacgtcg
     2101 cttcgaagcg atcgcatggt ggagatcgcc gtgtcagacc tcggcccagg ggttgcagaa
     2161 gaccagaagg aactgctatt tactcccttc catacgacca agaaggaagg aatgggtatg
     2221 ggtttatcga tctgccggtc gatcatgacc gagcacggtg gcgccctgag ctatcgcgac
     2281 aacgacggac ccggagcgac cttcttcttc acgctgccca catggcgcgc ggacgatgag
     2341 tgagcacgaa accacggttt tcgtcgtcga cgacgacgag ccggttcgcg acgccatcgg
     2401 gttgctgctc gaaaccgtcg aaattcccta cgaatccttc cctacggccc aggctttcct
     2461 ggacgcctac gatccgtccc gaaccggttg cctggtgctc gacatccgca tgcccggcat
     2521 gagcgggctg gagctccagg agcgcctgct ggaggacaaa gccccgatcc cgatcgtttt
     2581 catcaccggc cacggcgaca ttcccatggc cgtcgaggcc atgaagcggg gcgcgatcga
     2641 tttcattcgc aaaccctttc gggaccagga gctgctcgat cgcatacatg cggcgctcaa
     2701 cgcggacgaa gatcagcgcg gcgcgtacga caacctgacg accatccggg agagggtcgc
     2761 ggcgctcact ccgcgcgagc acgaggtatt cgaacgggtg gcccagggcc aggcaaacaa
     2821 ggtcgtcgcc atcgagctgg gcatcagcga gcgaaccgtg gagattcatc gaagccaggt
     2881 catgcagaag accgaagctc gaaccctggc cgacctggtg cgtatgaagc tgaccctgga
     2941 ccaggccgac gggccgtagc cggcagcagc tgaccggaga tcccggcccg gcgaccatcg
     3001 cggagagccg ttggagcgcg gggttccccg aagggactag tcgaacccga cccgatctgc
     3061 tcggatgcgc tgcgagaacg ccgcatcgcc gtgcgcgtag agcgccaccg ggttgatcag
     3121 cgcacgctcc ctgagctctt caaacggcag gccgtctccc agaataccgc cggtttcgta
     3181 tatcagctcg tcaaagcgtc cattggcgag cattcggatg tcgaacgggg cattgcgaaa
     3241 ctccacggtc tgcgcgagca ggctggtggt gcagttggcg ctgatcacgt tgtagaagcg
     3301 cggctggcgt gcgagcaggt tggtctcccg aaccgagtcg agaagaacga acagcgcctg
     3361 ctcgcgcggg aagctggtgc ggtacagata cacgggctca tcacgtacgt tggtccgtac
     3421 ccggacgaag tcgcgctcat cgccccacag gtactgcagc tcgaacatct tgtagagccc
     3481 gccgagcttc gagtaggcct catcgacctc gcggcgcgtt tccacggaca gcgcaacgta
     3541 accgtcagct ccgaaatcga aggagagcat ggggtgggct atcaggtcac cgccgaacgc
     3601 atcgtgaaag taatccatgc cgcgaagatt gcgaatactg accgtgcggg tttcccagga
     3661 ctccaccacc gaaccgtcgg cgccgtagtc gaagtttcgg aaattgtgaa actcgagcgc
     3721 accgtcgctg aactccaccc atccggtctt ggcccactcg ggtttccacg gcctctcgtt
     3781 ggaagggttc ttggccgtcc agggaatcag caccactaga aaccagacca gccagacgag
     3841 atatttcttc caggcaatcc ggatcagagc caggcacgcc gcggcgaccg caagccacgc
     3901 aacagccagc cagatgttgc cgtcgtccat gcctaacggt ccgtccacgt aaacggcgcc
     3961 cgcagcccac aggctggcaa acccgaccac cgtccacctg atgccccttc tgagcacgag
     4021 cccgatacgg ccctcggcat ccaaatcatc gaagttctgc ttaccggtcg aatgcatctg
     4081 ttcggaaaga ctcaccatct cgaaaacaat gcgtccctcg cgggaaggac accaagccgc
     4141 cgcgccggat tctaccaggg tcaacggccg ggcccgaacg cctccgggcg atgcgttagg
     4201 tacatatccg tatttgcagg tatcggcccg cgccataagt tatcggccca gagtcattct
     4261 tcgtttcgga ttgtcatgaa cgctaacgtc tgcgtcgtcg acggcgaccc cgccgtacgt
     4321 gacagcctgg ccacattgat gcggttgagt ggtcacgaag tgtcgacctt tgccaccggg
     4381 caggagtttc tggacgccgc agaagccgat accgtgggct gcgtcatctg cgaggccgac
     4441 ctcccggaca ccacggggct ggccgtgttc ggcgcgctga ccgaaaagag cctggcagca
     4501 ccctttgccc tgctgatcag ccagagcgac cccgcccgag tagcacaagc ctatcgcgcg
     4561 ggcattcgca acgtgtttcc gaagccgctc gtccatcgcc ggatactgtc cttcgtctcg
     4621 agatgatggg ctggcacaat tgtcttccaa ctcgaccacg gtggattcgt ccatggagct
     4681 gaagaacata ctggtggccc tgcacgatcg ggaatcgatg ggaagcgcga tgaggaaggc
     4741 cgcccgcctg gagcacttca ccggggcaaa catcaccgtg gttcagacct gctgggacgt
     4801 ggtcagcgag gagcccaccc agcacttccc gcaggaagag atcgacacca tcgcctcgcg
     4861 catgaaggag gcggagctca acaacctgtc ggccgagctc gcgcgctaca gcgaacgggt
     4921 cgcggagctg aatgcggaga tcctgtggtc aaagcaccat gacgatgccg tggcccgctt
     4981 cgccattcaa tcgggatccg acctcatcgt cgctccccga cacgcccgcg gcggtgtgaa
     5041 gcgcctgctg ttgcccgaag agctcaagct cgccgcgcgc gctcatgctc cgctcctgct
     5101 gacttccgct tcgacctggc gcgaaccgac caacgtgctg gtcgcgctgg acgccgggga
     5161 ccgtcagcac gcagccctga acagcctgct catcgagtgg ggcaggaccc tcgcggaaac
     5221 tctggggggc gagcttcacc tggtggcggc gagcccgccg ccgagctcga gcgcgcccaa
     5281 ccagacgctg gaggagttcc agcgagaggc ggaagcacga cggatggacg cgctgcgcgc
     5341 cctggccgat gctcatcccc acgactacgg gtccctgcag gtcaaaaccg gagacgtctg
     5401 cgacgtcatc gacgaggttg ccagcgcatg cgaggtggac atcgtcgtga tgggcaccgc
     5461 cgcgcgcacg ggattgaagc ggctgttcat cggcaactcg gcggaatccg tgctcagcca
     5521 gctgaccgaa atcgacatcc tcacggtgcc cgattcccag cagagctgat ccgcgcgcct
     5581 gctaaatccc aggcaaacga agcgcgatca ccttctcgcc ctccccgggc gcgacaacgg
     5641 tcaccgccga ctccacatcg ccctcctcga gctttgcgcc tcccagcagg ctcaccctgg
     5701 ccacgatctc gagctccgca cccgcctcat ggcggaacac cacgtccacc ggcagac
//
LOCUS       DBNHOX010000386         4746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_930795, whole genome shotgun sequence.
ACCESSION   DBNHOX010000386 DBNHOX010000000
VERSION     DBNHOX010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_930795"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="AC16UZ_08595"
     CDS             complement(<1..229)
                     /locus_tag="AC16UZ_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246780.1"
                     /translation="MPVVLAAMLLLALLAGCASRPDGGKTIEQLRGVKPDMTEVAIDD
                     SLNKALASYRHFLNETPVNNKTPDAMRRLADL"
     gene            complement(229..2109)
                     /locus_tag="AC16UZ_08600"
     CDS             complement(229..2109)
                     /locus_tag="AC16UZ_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6246781.1"
                     /translation="MTRPLAWTIGLQLALMLLCSPARADDVRDLYFGEALFQAGQGKY
                     FEALERLDAELGQHYGLDEPELDTLHHHIREAEFFVGDFELNYRMHHRAGRAISAVLN
                     GDVDQSVRNEAAYRLARIHFQKAQMADALEALARIEGSMPARVRDDVEFLRANVYLAV
                     GRSAEAAEVLRELQGTKGLTGFSEFNLAVALLQDGRTAEALQQLDRAGQVDARDAMTH
                     SIRDKANLVLGGMLLESGRFEEARAPLERVRLEGPMSNEALLGAGWAELSGENFERAV
                     VPWSILADRDPTDAAVQEAKLALPYAYSRLNVHGRAAVLYGKALDTFGRELNKLDASM
                     GSIENGSFLEALVREEIHQDKDWVVRMRSLPETPETFYLVELLASHDFQTALQNYLDL
                     EDLRKKLATWQASFDAFDSVIAARADYFEPLLPEVDQTFRELDSRFRVRVEQYELLVK
                     RMQDLLVMPRPEFLATTEEKVLSTGLTAMDEALGGVGRDGNDELRQRIRRLEGIITWT
                     LHTQYDDRLTRFYENLQDMQGSVALLKEHYQAFVRTRQAATLSYQGYDAPIKRLRGRI
                     HQASAKVDQLMARQGRMLEQVAMAELESRQRRLVNYQHQARYALADSYDRATQAQYAE
                     EP"
     gene            complement(2112..2618)
                     /locus_tag="AC16UZ_08605"
     CDS             complement(2112..2618)
                     /locus_tag="AC16UZ_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246782.1"
                     /translation="MLTALACLCWSPVSADDVSRKEMRSLDEQVQEIKSDVLSIAAEL
                     DQLEERLLFPSNTQLTVFVAIGDGTESFRLDSIRLGINDALATHHIYSFKELEALQQG
                     GVQRLYMGNLPIGTHALEVSVLGKLPSGKDFAHAERFTFDKGIDPALIGITVDPGSGK
                     APIALKDW"
     gene            complement(2856..3857)
                     /gene="gap"
                     /locus_tag="AC16UZ_08610"
     CDS             complement(2856..3857)
                     /gene="gap"
                     /locus_tag="AC16UZ_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR6246783.1"
                     /translation="MRVAINGFGRIGRSVFRIAETLGDIEIVAINDLFDDEALRYLLT
                     YDTVMGRFEGDVRIEDGHMITPKSRARMLALADPTQLPWAELEIDAVVESTGVFRSRE
                     QVSQHLEAGAGRALLTVPAKDDIDYTVVLGVNGEGLKAEHRVVSNASCTTNCLAPLAK
                     VLHDAFGIREGVINTVHAYTNDQRLADVPHSDWRRSRAAAENIIPTSTGAAKAVGVVL
                     PELAGRLDGIASRVPVPDGSVVDLFVELEKNVSVDDVHEVLEAAAGSEPMSGVLEFTR
                     EPIVSSDIIGNPHSSIYDASFTQVAGGRFLKALSWYDNEWGYSNRVCDVLRMMSALD"
     gene            complement(3857..>4746)
                     /locus_tag="AC16UZ_08615"
     CDS             complement(3857..>4746)
                     /locus_tag="AC16UZ_08615"
                     /inference="COORDINATES: protein motif:HMM:NF014818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246784.1"
                     /translation="HLLTRTDDDVGVTLSGNYLESRGVRTRLMPADHPTEIPWDVFGV
                     DVVIEATGRFHRADDLRPHLHNGAPRVVLSALPQDDIDRVVLVGVNDCEASAADVMVS
                     AGSASTTATALACKTITEVWPVEHATMTSVHAYTSDQSLQDYAGPDYRRSRSGAENII
                     PNATPALKWVQRAVPAMAGRMTGYALNVPVQVGSMLDLTFSFRESGIGADDVNDLFGN
                     AARVRPDLIATTRDPIVSSDVRGSSESLLVDMEGTMAAGSRMVKVLAWHETLGHARRI
                     LDVVGLYADLDDATAKEAS"
BASE COUNT          778 a         1499 c         1598 g          871 t
ORIGIN      
        1 gcagatccgc aaggcgccgc atcgcgtccg gcgtcttgtt gttgacgggc gtctcgttga
       61 gaaagtgccg gtagctggcc agcgccttgt tgaggctgtc gtcgatggcc acctcggtca
      121 tgtcgggctt cacgccgcgc agctgctcga tggtctttcc gccatcgggg cggctcgcgc
      181 aaccggcgag cagcgcaagg agcagcatgg cggccagaac gacgggcatc acggctcctc
      241 cgcgtactgc gcctgggtgg cgcggtcata gctgtctgcc agggcgtatc gtgcctggtg
      301 ctggtagttc accaggcgcc gctgccgtga ctccagctcc gccatggcaa cctgctcgag
      361 catccggccc tgacgggcca tgagctggtc cacctttgcg gaagcctgat gaatccgccc
      421 gcgaaggcgc ttgatgggcg cgtcgtatcc ctggtagctg agcgttgcgg cctggcgggt
      481 gcggacgaac gcctggtagt gttctttcag cagggcgacg gatccctgca tgtcctggag
      541 attctcgtag aagcgggtca gccgatcgtc gtactgcgtg tgcagcgtcc aggtgatgat
      601 cccttccaga cggcggatgc gctgacgtag ctcgtcgttc ccgtcgcggc ccaccccgcc
      661 aagagcctcg tccatggcgg tcagacccgt gctcaggacc ttctcctcgg tggtggccag
      721 aaactcggga cggggcatga ccaggaggtc ctgcatgcgt ttcaccagca gctcgtactg
      781 ctccacgcga acgcgaaagc gggagtcgag ctcgcggaac gtctggtcga cctcggggag
      841 caggggctcg aagtagtcgg cccgggcggc tatgacgctg tcgaaggcgt cgaagctcgc
      901 ctgccaggtg gcgagtttct ttcgcaggtc ctccagatcc aggtagttct gcagagcggt
      961 ctggaaatcg tgggaggcga gcagctctac caggtagaag gtttcggggg tctcgggcag
     1021 gctgcgcatg cgaaccaccc aatccttgtc ctggtggatc tcctcgcgca cgagcgcctc
     1081 gaggaacgac ccgttctcga tgctacccat ggaggcgtcg agcttgttga gctcccggcc
     1141 gaaggtgtcg agcgccttcc cgtagagaac ggccgctcgt ccgtgaacgt tcaggcggct
     1201 gtaggcatat ggcagtgcca gcttcgcttc ctgcacggct gcgtccgtcg ggtcgcgatc
     1261 ggccagaatg gaccagggca ccacagcgcg ctcgaagttc tcgccggata gctcggccca
     1321 gccggcccct aagagcgcct cgttggacat gggcccttcc aggcgaacgc gctccagagg
     1381 cgcccgggcc tcctcgaacc gccccgactc cagcagcatg ccgccgagca ccagattcgc
     1441 cttgtctcga atggagtggg tcatggcgtc cctggcgtcg acctgacctg cccggtcgag
     1501 ctgctgcagg gcctcggccg tgcggccgtc ctgaagcaga gcaaccgcca ggttgaactc
     1561 ggaaaagccg gtgagcccct tcgttccctg cagctcccgg agcacctcgg cggcttcggc
     1621 cgagcggccg acggcgaggt agacgttggc gcggagaaac tccacgtcgt ctcgaacgcg
     1681 cgcaggcatg gatccctcga tgcgcgcaag cgcttccagg gcatcggcca tctgggcttt
     1741 ctgaaagtga atgcgtgcca gcctgtaggc ggcttcgttg cgcacggact gatccacgtc
     1801 gccgttcaag accgcgctga tggcgcggcc cgcgcgatga tgcatgcggt agttgagctc
     1861 gaagtcgccg acgaagaact cggcttccct gatgtggtgg tgcagggtgt ccagttccgg
     1921 ctcgtcgaga ccgtagtgct ggccgagttc cgcgtcgagt cgttccagcg cttcgaagta
     1981 cttgccttga ccggcctgga acaacgcctc gccaaaatac aggtcgcgga cgtcatcggc
     2041 gcgcgccggg ctgcaaagca gcatcaaggc cagctgcaac ccgatggtcc aggcgagggg
     2101 tctggtcatg gctaccagtc tttcagggcg atcggggcct tgccggagcc cgggtcaacg
     2161 gtgatgccga tcagagccgg gtctatgccc ttgtcgaagg tgaagcgctc ggcgtgggca
     2221 aagtccttgc cgctcggcag cttgcccagc acggaaacct ccagcgcgtg agtgccgatg
     2281 ggcaggttgc ccatgtagag acgctgaacg cctccctgct gcagcgcctc gagttccttg
     2341 aagctgtaga tgtggtgagt ggcgagcgca tcgttgatgc cgagccggat cgagtcgagc
     2401 cggaaagact ccgtcccgtc gccgatggcg acgaagacgg tcagctgggt gttggacgga
     2461 aagagcagcc tctcctcgag ctggtcgagt tccgcggcga tgctcagcac gtcggatttg
     2521 atttcctgaa cctgctcgtc gaggctgcgc atctccttgc gggaaacgtc gtcggccgaa
     2581 accggcgacc agcacaggca cgccagcgca gtcagcaatg cgattattgg ggtctttggc
     2641 ttctcgaaca cctaatgacg cggtgcgtgg aatgcctctc gcgtctccac agtccacccg
     2701 cgtttgtccc tgttccggcg caagtcttga cgaatcgtgc cgcgcggtgt gtgactgagc
     2761 gctcaaagca ggccgcgggc ccgggaacgg gcccgtaagc ttgcgctatt cagcgtttga
     2821 agggtgtaga cgtctcagat tttcgaggac gcgggtcagt ccagggcgga catcatccgc
     2881 aggacgtcgc acacccggtt cgagtaaccc cactcgttgt cgtaccagga aagcgccttt
     2941 aagaaccgcc ccccggccac ctgagtaaac gacgcatcgt agatcgacga gtgcgggttg
     3001 ccgatgatgt ccgaagagac gatgggctcc ctcgtgaact ccagcacgcc gctcatcggc
     3061 tcggaaccgg cggcagcctc cagcacctcg tgaacgtcgt ccacgctcac gttcttctcg
     3121 agctccacga aaagatccac caccgagccg tccgggacgg gaacgcggga agcgatgccg
     3181 tcgagcctgc ctgcaagctc cggcaggacg acgcccaccg ccttggctgc gccggtggag
     3241 gtcgggatga tgttctcggc tgcggcccgg ctgcggcgcc agtcggagtg gggcacgtcg
     3301 gccagccgct ggtcgttggt gtaggcgtgc acggtgttga tgacgccttc gcggatgccg
     3361 aaggcgtcgt gcaggacctt cgccagaggt gcgaggcagt tggtggtgca gctggcgttg
     3421 gaaacgaccc ggtgctcggc cttcaagcct tccccgttga cccccagcac cacggtgtag
     3481 tcgatatcgt cctttgccgg cacggtcagc agcgcgcgcc cggcaccggc ctcgagatgc
     3541 tgggaaacct gctcgcggct gcggaacacg cccgtggact ccaccaccgc gtcgatctcg
     3601 agctccgccc atggcagctg tgtgggatct gcaagcgcga gcattcgtgc ccggctcttg
     3661 ggcgtgatca tgtgcccgtc ctcgatgcgc acgtcgcctt cgaatcgacc catcacggtg
     3721 tcgtaggtga gcaggtagcg aagcgcctcg tcgtcgaaga ggtcgttgat ggcgacgatc
     3781 tcgatgtccc ccagcgtttc cgcgatgcgg aagaccgagc ggccgatgcg gccgaagccg
     3841 ttgatggcga ctcgcatcag gaagcctcct ttgctgttgc gtcgtccagg tcggcgtaga
     3901 ggccgacgac gtccaggatg cgccgggcat gcccgagggt ttcgtgccag gcgagcacct
     3961 tcaccatgcg actgccggcc gccatggttc cctccatgtc gacgaggaga gactcggagg
     4021 agcccctgac gtcggatgaa acgatggggt cccgggtggt ggcgatcaga tccggccgta
     4081 cccgtgcagc gttgccgaac aggtcgttca cgtcgtcggc gccgatgccg gattcccgga
     4141 acgagaaggt gaggtcgagc atcgagccga cctggacggg cacgttgagc gcgtacccgg
     4201 tcattctgcc tgccatcgcc ggcacggccc gctgaaccca cttcagggcc ggggtcgcgt
     4261 tggggatgat gttttccgct ccggagcggc tgcgccgata gtccggtccg gcgtagtcct
     4321 gcaggctctg atcgctggtg taggcgtgca cggacgtcat ggtggcatgt tccacgggcc
     4381 agacttcggt gatcgtcttg cacgccagcg cggttgcggt ggtggaagcc gacccggcgg
     4441 acaccatgac gtcggccgcg cttgcctcgc agtcgttcac gccgacgagc accacgcgat
     4501 cgatgtcatc ctggggaagt gccgacagga cgacgcgcgg agcgccgttg tgcaggtgag
     4561 gccgcagatc gtcggcgcga tgaaacctgc cggtcgcctc gatgaccacg tcgaccccga
     4621 agacgtccca gggaatctcc gtgggatgat cggccggcat caggcgggtg cgaacgcccc
     4681 tggattcgag gtagttgccg cttagcgtca cgcccacgtc gtcatcggtc ctggtcagca
     4741 gatggt
//
LOCUS       DBNHOX010000387         1844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_352240, whole genome shotgun sequence.
ACCESSION   DBNHOX010000387 DBNHOX010000000
VERSION     DBNHOX010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_352240"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="AC16UZ_08620"
     CDS             <1..282
                     /locus_tag="AC16UZ_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246785.1"
                     /translation="YGPVAGDAPIGEGEVFYKTRGARRGESRLIDAPPREQRRVTLIA
                     GPHEGATILYTAYGGPLAPREPFDVLDQPEEVQRESNTFWAEHALSAQV"
     gene            complement(279..1097)
                     /locus_tag="AC16UZ_08625"
     CDS             complement(279..1097)
                     /locus_tag="AC16UZ_08625"
                     /inference="COORDINATES: protein motif:HMM:NF037897.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyltransferase-containing protein"
                     /protein_id="HIR6246786.1"
                     /translation="MPKSDEEVWYEWRSLVNMTAGELQRFLDEWGDVAGLSRAEAKAQ
                     GIRSGRDSARAILRMLPKGGRSYPQAERNWTTTDWEWARRQVGFIKRMRGARGALYDA
                     KGEPTRKLLALLVWGHDPEKMEPNPIAYHGTPHMFAPSDARIGWGMFGPGLYLTSDRK
                     TAEGYATGEAWGDEEWVREHGQPAVLVCDVELRKPIEWSWDRFLRDGFGWVDGRVRRI
                     MEDNEDLRAEVAIAKLQKLGYDGVIVPPAEPDGHVEYVVFDPTRLVCVREERIA"
     gene            complement(1522..>1844)
                     /locus_tag="AC16UZ_08630"
     CDS             complement(1522..>1844)
                     /locus_tag="AC16UZ_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246787.1"
                     /translation="IPHAALGMVFQASGAPPWLAIGSHVVFEFVEDGIKEAVQPIWPD
                     TTPDAWENHVGDVASFTGGYYAATALRHSDSGKALLTGFVAVAAGIWMWNLVQRHSWA
                     RTST"
BASE COUNT          323 a          630 c          542 g          349 t
ORIGIN      
        1 tacgggcccg tcgctggcga cgcgccgatc ggggagggcg aggtcttcta caagacacgc
       61 ggcgctcgac gtggcgagag ccggctcatc gacgctcctc cccgggagca gcgcagggtg
      121 acgctcatcg ccggacccca cgagggcgcg accatcctct acaccgccta cggcggcccg
      181 ttggcgcctc gtgagccgtt cgacgtgctc gaccaaccgg aggaggtcca gcgagagtcg
      241 aacacattct gggccgagca cgcgctcagc gctcaggtct aggcaatgcg ctcctcgcgc
      301 acgcaaacga gtcgcgtcgg atcgaagacc acgtactcca cgtgaccatc aggttccgcc
      361 ggcggaacta tcacgccgtc gtagcctagc ttttgcagct ttgcgatggc gacctcggcc
      421 ctcaagtcct cattgtcctc catgatgcgg cggacacgac catccaccca tccaaagccg
      481 tcccgcagaa accggtccca ggaccattcg atcggcttgc gaagctcaac gtcgcaaaca
      541 agcaccgcgg gctgcccgtg ctctcgtacc cactcctcat ctccccatgc ctcccctgtt
      601 gcatagccct ccgccgtctt acggtccgac gtcaggtaca gccccggacc aaacataccc
      661 cagccgatgc gcgcgtcgct cggtgcgaac atgtgtggcg tcccgtggta ggcgatcggg
      721 ttcggttcca tcttctctgg atcatgtccc cacacgagca gtgccagcag ctttcgcgtc
      781 ggctcgccct tcgcgtcgta gagcgctcct ctcgcgccgc gcatccgctt gataaagccc
      841 acctgacgtc gagcccactc ccagtctgtc gtcgtccagt tgcgctccgc ctgcgggtac
      901 gagcggccac ccttcggcag catgcgcagg atggcccgcg cgctgtcgcg gccggagcgg
      961 atgccttggg cctttgcttc tgcgcgcgat aggcccgcca cgtcacccca ttcgtcgaga
     1021 aagcgttgta gctctcccgc tgtcatgttc accagcgagc gccactcgta ccagacttcc
     1081 tcgtcgctct tgggcaccgg cgcctctggt tatgcgagcc taacacgtaa aaaggcccgt
     1141 caccacccat ggtggcgcaa ggaaacgata gaggaaaccc ctcggttgat caagaccgag
     1201 gggctccagt tcccaccgcc gatgccgctt accgggccgg cgatgtcgac gtgtgccggt
     1261 gaccagcttt ggcgaaggac aggcgggtga cggtaagtat cgcctggggt aaacttcatc
     1321 gtcaagcggc tttgcaaatg gaacctcact gcattagcga cgagcagcga gcgacgcaca
     1381 tcatattcga ggctgccgcg attttgatcg tgaccccgtt cctggcgtgg ctggcgacgc
     1441 gacgagagct tccgcctgca gccagggcgc tcagcggcgg catagctgtc gtcacggtca
     1501 ttgtggacgg cgctctgctg tctaggtact tgtccgtgcc cacgagtgtc tctggactag
     1561 gttccacatc cagattccag cagccacggc aacaaatcct gtaagcaacg cttttccact
     1621 gtcgctatgt ctgagcgcgg tggctgcgta gtatccgccg gtaaagctgg ccacgtcgcc
     1681 gacatgattt tcccatgcgt ccggggttgt gtctggccag atcggctgga ctgcctcttt
     1741 gatgccgtcc tcgacgaact cgaagacgac gtgcgatccg atcgccaacc acgggggcgc
     1801 tccggacgcc tggaatacca ttccgagggc tgcatgcgga atgg
//
LOCUS       DBNHOX010000388         1872 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1059525, whole genome shotgun sequence.
ACCESSION   DBNHOX010000388 DBNHOX010000000
VERSION     DBNHOX010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1059525"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="AC16UZ_08635"
     CDS             <1..1195
                     /locus_tag="AC16UZ_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR6246788.1"
                     /translation="ALPSFIDMKAFDALPGDSIADLGMPPSEVTVAEVLQQAGYYTAM
                     VGKWHLGNVGGMQPHEQGFDEALELAGMLYLPEDHPDAVNAKRMDEPIERMVWASARY
                     AARFNGGERFEPDGYLTDYYTAEALQVIESNRHRPFFLYLAHWGIHNPLQATRADYDA
                     YSHIEDHHLRVYSAMIRAVDRSVGLISGKLDELGLADNTLILFTSDNGGASYIQLPEV
                     NKPYRGWKLTHFEGGTHVPFMARWPARIAPGSSFDAPVHHVDLFHTIVAAAGAAIPRD
                     RKLDGVDLVPYVTGQAAGVPHETLFWREGHHQSVQHRGWKLIRSARPDKRWLFDLAND
                     PTEQVDLAGARPDKVEELVALLDAHNAEQSDPMWPSVLDSPMLVDKHGGQPYEEGDEY
                     IYWPN"
     gene            1225..>1872
                     /locus_tag="AC16UZ_08640"
     CDS             1225..>1872
                     /locus_tag="AC16UZ_08640"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HIR6246789.1"
                     /translation="MDAVRTPDDRFENLADYPFAPDYVNVDDGQGGALRMHYVDEGEG
                     EVVLCLHGQPSWSYLYRKMIPLLVDAGYRAVAPDLIGFGKSDKPTSRANYTYAAHVHW
                     LNSFVEALDLKQVTVIGQDWGSLIGLRVVAENPDRFARIVLANGALPDAKGIPEAMAP
                     QLKAMLADTPALPIEEMFAKLSGPMEDRPPFMYWVRYCDENPDFHPADVMKGSLAH"
BASE COUNT          365 a          600 c          588 g          319 t
ORIGIN      
        1 cgcattgccg agcttcatcg acatgaaggc tttcgatgcg ctgcccgggg acagcatcgc
       61 ggacctgggc atgccgccca gcgaggtcac cgtcgccgag gtgctgcagc aggccggcta
      121 ctacacggcc atggtgggca agtggcacct gggcaacgtc ggcggcatgc agccccacga
      181 gcagggcttt gacgaggctc tggagcttgc cgggatgctt tacctgccgg aagatcatcc
      241 cgatgcggta aacgccaagc gtatggacga gccgatagag cggatggtct gggcgagtgc
      301 gagatatgcc gcgcgcttca atggcggcga gcgcttcgag cccgacgggt acctgaccga
      361 ctactatact gccgaagcac tccaggtcat cgagagcaac cgacaccgac cgttctttct
      421 ctatctcgcc cactggggaa tccacaaccc gctgcaggcg accagagcag actacgatgc
      481 gtactcgcac atcgaagacc atcatctgcg cgtctactcg gcgatgatcc gggccgtgga
      541 ccgcagcgtg ggtctgatat ccggaaagct ggacgagctc gggcttgccg ataacacgct
      601 gatcctgttc accagcgaca acggcggtgc ttcctacata cagctgcccg aagtcaacaa
      661 gccctatcgc ggctggaagc tgacccattt cgaagggggc acccacgtgc ccttcatggc
      721 tcgatggccg gctcgcatcg cgccgggatc gagtttcgat gcgcccgtcc atcacgtgga
      781 cctgttccac accatcgtcg ccgcggcggg cgcggccatt ccgagggacc gaaagctcga
      841 cggcgtggac ctcgttccct acgtcaccgg tcaggcagcc ggcgtgcccc acgaaaccct
      901 gttctggcgg gaagggcacc accagtcggt tcagcatcgg ggctggaagc tgatccgctc
      961 ggcccggccc gacaagcgct ggctgttcga tctggccaac gatcctacgg agcaggtcga
     1021 tctggccggg gcacggccgg acaaggtcga ggagctcgtg gcgctgctgg atgcccacaa
     1081 tgccgagcag tccgacccca tgtggccatc ggtgctggat tcgccgatgc tcgtcgacaa
     1141 gcatggcggc cagccttacg aggaaggcga cgagtacatc tactggccca actgaaaccc
     1201 gcaaacagat caggaataag aacgatggac gccgtcagaa ccccagacga tcgatttgaa
     1261 aatctcgccg actacccctt tgcccctgac tacgtgaacg tcgacgacgg ccaaggcggg
     1321 gcgttgcgca tgcactacgt tgacgaaggt gaaggagagg tcgtcctgtg cctgcatggc
     1381 cagccgagct ggtcctatct gtaccgcaag atgatcccgc tgctcgtcga tgccggatat
     1441 cgggccgttg cgcccgatct gatcggcttc ggcaagtccg acaagcccac gagccgcgcg
     1501 aactacacct acgcagcgca cgtgcactgg ttgaatagct tcgtcgaggc cctggacctg
     1561 aagcaggtca cggtcatcgg ccaggactgg ggcagcctca tcgggttgcg ggtcgtggct
     1621 gaaaaccccg accgttttgc ccgcatcgtg ctggcgaacg gcgcgctgcc cgacgccaag
     1681 gggatcccgg aggccatggc gcctcagttg aaggccatgc tggccgacac gcccgcgctg
     1741 ccgatcgagg agatgtttgc gaagctctcc gggcccatgg aagaccgccc gccgttcatg
     1801 tactgggtga gatactgcga cgagaatccg gactttcacc cggctgacgt catgaagggt
     1861 tcgctggccc ac
//
LOCUS       DBNHOX010000389         5131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1765804, whole genome shotgun sequence.
ACCESSION   DBNHOX010000389 DBNHOX010000000
VERSION     DBNHOX010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1765804"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="AC16UZ_08645"
     CDS             <1..223
                     /locus_tag="AC16UZ_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019690203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR6246790.1"
                     /translation="GVTCGITFTFGGLHTDSRARVIDQGGEPIEGLFAAGELVGGLFY
                     FNYPGGTGLTSGSVFGRIAGREAAGGRSA"
     gene            complement(245..2155)
                     /locus_tag="AC16UZ_08650"
     CDS             complement(245..2155)
                     /locus_tag="AC16UZ_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012271055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR6246791.1"
                     /translation="MSRRKRQAIAVVTDFAVLPIALWTAIALRLGQVNPEVAQFWPAF
                     VVSSLLCIPVFGALGLYRHVVRHTGSEAIWAIVKGATIIALVVAAVAYMIPLPGFPRS
                     VPIIFWLLTLLYVSGTRFSMRAYFQWLQNRVRLRSPAVIYGAGSKGVELARVLSQQGD
                     YLPIAFIDDDRALHKRVIDGLNVYPPKSLGRVLRDSGARDVMVAIGSAEPDERRRIIE
                     FLEPFPARVRLIPDLVELESRRQTIANIRDVEIEDLIGRNTVSPLPHLLRGSVEGRSV
                     LVTGAGGSIGSELCRQIVRQGPQRMVLLDQSEFGLFEIQRELTQLIVSEGLPVDVVAV
                     LGSVTNGTLMRRNMESYAVETVYHAAAYKHVDLVEHNIIQGLRNNTFGTLYTAQAAMD
                     AGVREFILISTDKAVRTTSVMGASKRLAEMVLQALQEISDSTRFSMVRFGNVLGSSGS
                     VVPLFAEQIAQGGPVTVTHPEVTRYFMTIPEAAELVLQAASMAEGGDVFLLDMGEPVK
                     IRELAERMIRLKGLTVRTSSQSGGDVEIRITGLKPGEKLHEELLVGDEVIGTEHAKIM
                     RAEETFLPWPALRGALNTLEQACDAYDYDAIKQFIEGLVEGASLESQLGHLNAGPRVL
                     QLDPNRESPSQP"
     gene            complement(2184..3167)
                     /locus_tag="AC16UZ_08655"
     CDS             complement(2184..3167)
                     /locus_tag="AC16UZ_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIR6246792.1"
                     /translation="MVSGTATGYLIAGFAASTVLVFLMLRVGRALNLMAVPNARSSHV
                     VPTPTAGGLGFVIPLLVFFGMLYLDGVDVALGLLLSLVLVAAASLWDDFAELSAGLRF
                     TCQTAAVALIVWQLDLPWAWYLLGLTGLALLWHVNLFNFMDGIDGIAAGQCLLYVVGV
                     QVLTLGAPGWMGELCWLTAGAMLGFLAYNWPPAKIFMGDVGSAVLGLLLALLALLLAR
                     DGVLPLIASLILLAGFWFDATYTLCVRMATGQAFTQAHRSHLYQKLAAFKGHLWTTGV
                     FIAFGLFWLLPLAWLTLRVADLAWVWLVAAVVPIGVASMRFGAGLPAQDEG"
     gene            complement(3137..4066)
                     /locus_tag="AC16UZ_08660"
     CDS             complement(3137..4066)
                     /locus_tag="AC16UZ_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6246793.1"
                     /translation="MRRAVVTGAGGFIGTHLCRALRHEGWEVAVITRAHALPGCERLP
                     VTLASADVELSRALGGAQVVFHLAGLAHGRASGAAEDELHRVNVESSARLLAASEAAG
                     VPAMIWLSSIKVLGDTSERPLRPEDAYRAGDAYARSKVEGERALLDREGFTRAAIVRP
                     PLVYGPGVAGNFASLARWVGSGVPLPLGAASAPRSMVGVDNLCSLMLRLAEEGSGVFH
                     VADARDRSVAQLIRAMAVLLGVPDRSWRVSPRLLAAVASALGQRARYTRLFEPLQVDQ
                     SMTRERLGWQPPHDPDQQLKETMRWFRERRQAT"
     gene            complement(4063..>5131)
                     /locus_tag="AC16UZ_08665"
     CDS             complement(4063..>5131)
                     /locus_tag="AC16UZ_08665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6246794.1"
                     /translation="ALAVEAKRHEVEVVVVCGANTGEQGLADAGLRYVTVPMSRSGFN
                     PFKELRTAAALLRIYRAEKPDLVHHVTIKPVLYGTSMARWSGVPAVVNAVPGMGFVFT
                     RRGPLARIRRSVVQLLYRLCMFHPNMRVIFQNNEDMQAFTQRGIVDRQHVALIRGSGV
                     DLDVFRCAPEPPGPVTFVLVGRMLRHKGVYEYVEAARRVRQRRPDWRFQLVGDVDPGN
                     PASLRPDELIAWQQAGEIEWLGQRRDVPEVLAAAHVVCLPSYREGLPKTLLEAAACGR
                     AMIASDIAGCREVVRPNVTGLLVPPRVSEPLADAMLRLGEDDALRHRFARAARSAAEE
                     VFSLEDVVRHTFRVYDELLLP"
BASE COUNT          991 a         1734 c         1639 g          767 t
ORIGIN      
        1 cggcgtcacc tgcggcatca ccttcacctt cggcggcctg cataccgact cgcgagcccg
       61 ggtcatcgat caggggggcg agcccatcga agggctgttt gccgccggcg agctggttgg
      121 cgggctgttc tatttcaact acccgggtgg taccgggctg acgtcaggtt cggtgttcgg
      181 gaggatcgcc ggccgcgagg cggccggcgg gcgttctgct tgaggccgtg gccagatcgc
      241 cggttcaggg ttgcgacggc gactcgcggt tggggtccag ctgcaggacg cgcggtccgg
      301 cattcaggtg cccgagctgg ctctccaggc tcgccccttc cacgaggccc tcgatgaact
      361 gcttgatggc atcgtaatca taggcatcgc aggcctgttc gagggtgttc agcgcgcccc
      421 tgagcgccgg ccacggcagg aacgtctcct cggcgcgcat tatcttggcg tgctcggtgc
      481 cgatcacctc gtctcccacc agcagctcct cgtggagctt ctcgccaggc ttgagtccgg
      541 tgatcctgat ctccacgtct ccacccgatt gagaggaagt gcgcaccgtc agccctttga
      601 gcctgatcat ccgctccgcg agctcccgga tcttgacggg ttcgcccatg tcgagaagga
      661 aaacgtctcc accttcggcc atgctggcgg cctgcaggac gagctccgcc gcctccggaa
      721 tggtcatgaa gtagcgggtg acctcgggat gggtgacggt gaccggcccg ccctgggcga
      781 tctgctcggc aaacagcggc accacggatc ccgacgagcc caggacattg ccgaagcgca
      841 ccatggagaa gcgggtactg tcggatattt cctgcagcgc ctgaaggacc atctcggcca
      901 gccgcttgct cgcgcccatg acgctggtgg tgcgaacggc tttgtcggtg gagatgagaa
      961 tgaactcgcg cacgccggcg tccatggccg cctgcgccgt gtaaagggtg ccgaaggtgt
     1021 tgtttctgag cccctggatg atgttgtgct cgacgagatc cacgtgcttg taagccgcgg
     1081 cgtggtaaac ggtttctacg gcgtagctct ccatgttgcg ccgcatgagc gtgccgttgg
     1141 tgacagagcc gagcaccgcc acgacgtcca cgggcagccc ttccgatacg atcagctgcg
     1201 tcagctcgcg ctggatctcg aacagcccga actcggactg atcgagcagc accatgcgtt
     1261 gtgggccctg acgaacgatc tgccgacaca gctccgagcc gatagacccg ccggctccgg
     1321 taacgagaac gctgcgccct tccaccgaac cgcgaagaag gtgcggcaac ggagataccg
     1381 tgttgcgacc gatcaggtcc tcgatctcca cgtcccggat gttggcgatg gtctgccggc
     1441 gtgactccag ctccacgaga tcgggaatca agcgcacgcg agcgggaaac ggctccagaa
     1501 actcgatgat gcggcgccgc tcgtccggct ccgcagaccc tatcgccacc atgacgtcgc
     1561 gcgctccgga atcacgcagc acccttccga gcgatttagg cgggtagacg ttcaacccgt
     1621 cgatgacgcg cttgtggagc gcacgatcgt catcgatgaa ggctatcggg aggtagtcgc
     1681 cctgctggct cagcacccgc gccagctcca cgcccttcga cccggctccg tagatgacgg
     1741 cgggactgcg caggcggacc cgattctgca gccattggaa gtaagcgcgc atggagaaac
     1801 gggtgccgct gacgtagagg agggtgagca gccagaagat gatgggaacg gatcggggga
     1861 atccgggcag cgggatcatg taggccacgg cggccacaac cagggcaatg atggtcgcgc
     1921 ccttgacgat ggcccagatc gcctcgctgc ccgtatggcg gaccacgtgg cgatacaagc
     1981 caagagcacc gaaaaccggg atgcacagca ggctgctgac gacgaaggcc ggccagaact
     2041 gcgccacctc cggattgacc tgccccaggc gcagggcgat cgctgtccag agcgcgatgg
     2101 gaagcacggc aaagtccgtg accacggcaa tagcctgccg cttgcgccgg gacaactgat
     2161 tgatgaaatt ctcgatgatc ttctcagccc tcgtcctggg cgggcagccc cgcgccgaaa
     2221 cgcatggacg ccacgccgat gggaaccaca gcggccacaa gccagaccca tgccagatcc
     2281 gcaacgcgca acgtcagcca ggcaaggggc agcaaccaga acaagccgaa ggcgatgaac
     2341 acaccggtag tccagagatg gcccttgaat gccgcaagct tctgatacag gtggctgcga
     2401 tgcgcctgag tgaacgcctg accggtcgcc attctgacac acagcgtata cgttgcgtca
     2461 aaccagaacc cagcaagcag tatgagagag gcgatgagcg gcagcacgcc gtcgcgcgcc
     2521 agaaggagcg cgagcagagc aagcagcagg cccagcaccg cgctcccgac atctcccatg
     2581 aagatttttg ccggcggcca gttgtaggcc aagaatccca gcatggcgcc ggccgtaagc
     2641 cagcaaagct cgcccatcca gccgggcgct cccagggtga ggacctggac acccaccacg
     2701 taaagcaggc attgccccgc cgcgatcccg tcgatgccat ccatgaagtt gaacaggttg
     2761 acgtgccaga gcagcgccag ccccgtcaat cccagtagat accaggccca gggcagatcg
     2821 agctgccaga cgatgagcgc aacggcagcc gtctgacagg taaaccgcag accggccgaa
     2881 agctcggcaa aatcgtccca gaggctggcc gccgcgacga gaaccaggct gagcagcaag
     2941 ccgagggcca cgtcgacgcc gtcaaggtaa agcatcccga aaaaaaccag gagcggtatc
     3001 acgaacccca acccccccgc cgtcggcgtg gggacgacgt gggagctgcg cgcattggga
     3061 acggccatta ggttcaacgc ccggccgacc cggagcatca ggaagacgag caccgtcgag
     3121 gccgcgaacc ccgcgatcag gtagcctgtc gccgttcccg aaaccatcgc atcgtttcct
     3181 tcaactgctg atcgggatcg tggggcggct gccagccaag gcgctcccgg gtcatgctct
     3241 gatctacctg caggggctcg aacagccgag tgtagcgtgc gcgctgcccc aatgccgagg
     3301 cgacggccgc cagcagccgc ggcgaaaccc gccacgagcg gtcgggaacc cccagcagca
     3361 ccgccatggc acgaatcagc tgggcgacgc tgcggtcgcg agcatccgca acgtggaaca
     3421 cgccggagcc ttcctcggca agacgaagca tcaggctgca cagattgtcg accccgacca
     3481 tgctgcgcgg cgcagacgcc gctccgagcg gcaacgggac gccgctgccg acccaccgcg
     3541 cgagactcgc gaagtttccg gccacgccgg gcccgtaaac cagaggtggg cgcacgatgg
     3601 ccgcgcgcgt gaagccctcc cggtccagaa gcgcgcgttc cccctcgact ttgctgcgcg
     3661 cgtaggcatc cccagcccga taggcgtcct ctggtcgaag cggccgctcg ctcgtatctc
     3721 cgagcacctt gatcgagctc agccagatca tcgccggcac cccggcggct tccgacgcgg
     3781 cgaggaggcg ggcggaagac tcgacgttta cccgatgcag ctcgtcctca gccgcgcccg
     3841 atgcccggcc gtgagcaagt ccggcgagat gaaacaccac ctgtgcgccg ccgagcgccc
     3901 gcgacagctc gacgtcggct gaggccagcg ttacgggaag ccgctcgcat ccggggagag
     3961 cgtgagcgcg ggtaatgacc gccacctccc aaccctcatg acgaagcgcg cggcacaggt
     4021 gggtaccgat gaagccgccg gcgccggtga cgacggctct tctcacggca gcagcaactc
     4081 gtcgtagacc cggaacgtat gtcgaaccac gtcctcgagg gagaagacct cctccgccgc
     4141 gctgcgcgcc gcgcgggcaa agcgatgacg aagcgcatcg tcctcgccca ggcgcagcat
     4201 ggcgtcggca agcggctcgc tgacccgggg cgggaccagc agtcccgtca cgttcgggcg
     4261 gaccacctcc cggcatccgg cgatgtccga ggcgatcatg gcccgcccgc aggcggccgc
     4321 ttcgagaagc gtcttgggca gcccctcccg ataggacggc aggcagacca cgtgagccgc
     4381 agccaggacc tcgggaacat cgcgccgttg tccgagccac tcaatctcgc cggcctgctg
     4441 ccaggcgatc agctcatcgg gccgcagcga tgcaggattt ccaggatcca cgtcgccgac
     4501 gagctggaat cgccagtcgg ggcgccgttg cctgacccgg cgggcggcct cgacgtactc
     4561 gtaaacccct ttgtgccgga gcatccgtcc aacgagcacg aacgtcacgg ggccgggcgg
     4621 ctcgggagcg caccggaaca cgtccagatc gacgcccgat ccccgaatca gcgcaacgtg
     4681 ctgccggtct acgatgcccc gctgggtgaa cgcctgcatg tcctcgttgt tctggaatat
     4741 cacgcgcatg ttgggatgga acatgcacaa acggtaaagc agctgcacga cgcttcgtcg
     4801 gatgcgggca agcggcccgc gccgggtaaa cacaaacccc atccccggca cggcgttcac
     4861 cacggcgggc acgccgctcc agcgcgccat ggaagtgccg taaagaaccg gcttgatggt
     4921 cacgtgatga accaggtcgg gcttctcggc acggtagatt cgcagcagcg cggcggcggt
     4981 gcgcagttcc ttgaacggat tgaaaccgct tcgcgacatg ggcaccgtaa cgtagcgcag
     5041 ccccgcgtcc gccagtccct gctccccggt attggcgccg caaaccacca ccacctccac
     5101 ctcgtgacgt tttgcttcca cggccaaagc c
//
LOCUS       DBNHOX010000390         2417 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1124078, whole genome shotgun sequence.
ACCESSION   DBNHOX010000390 DBNHOX010000000
VERSION     DBNHOX010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1124078"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..468)
                     /locus_tag="AC16UZ_08670"
     CDS             complement(175..468)
                     /locus_tag="AC16UZ_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR6246795.1"
                     /translation="MKAVGIKVLKNKLSEYVRLAAGGETVLVTDRDRVVAQITAPGPT
                     VSPLLADAALADAVRNGHIRAPLLRTGQPPVAPPPAVKLSDVLEGLEQDRGER"
     gene            complement(665..2056)
                     /locus_tag="AC16UZ_08675"
     CDS             complement(665..2056)
                     /locus_tag="AC16UZ_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6246796.1"
                     /translation="MVKHASTDSAGRDAVPPLSRRTLFYYGLADMPIQMAAVPVAAFV
                     PNYYGQDLGVSLAAVGLVLLLSRSFDAITDPLIGWLSDRTDTRWGRRRVWMLASVPIL
                     MLAIYKLFMPDPGDASAVYLLIWLVVLWLGWTMLFIPYYAWAAELSPEYNERSRITGW
                     RSSIGLAANVLSKLVPVVAIFCCAYGGTQETLFLVGTMTLILLPLTVGLTVAMVPEGK
                     DFIPARMPLVPGLKIMWRNGPFKRLVAAFFVNQLGTSISTALIVFYVRSVLQDDDKTI
                     LQLLVYYGFNLCGIPFFVRYSGIVGKHRAWCIALLLFAFFMLGYLLLGAGDFYWMLPI
                     TAITGFLAGSFWVIPNSMKADVIDIDRLESGEDRTAWYFAVWSLATKVALSVGPAIAL
                     GLLSLTGYVPAAEAGAYEGSTLGLKLLFVFGPSTGLLLTALIAWRYPLTETKHRELRQ
                     ALARSRSRPRPAG"
     gene            complement(2040..>2417)
                     /locus_tag="AC16UZ_08680"
     CDS             complement(2040..>2417)
                     /locus_tag="AC16UZ_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6246797.1"
                     /translation="ALTGFLGGSFWVLPNSMKADVIDVDTLNTGEDRAAWYFAVWSFT
                     TKVGQSIGPWLALTLLAFVGYDPASQPEPTAGGLLGLKLLYVFGPACGFWLAALIAWN
                     HPLTESRHREIRASLEKVRGQAR"
BASE COUNT          474 a          766 c          822 g          355 t
ORIGIN      
        1 ccagccccct ggcggcttcg ccgtgggatg ccgcaaaccc catgacgtgc agccggttcc
       61 acagctcgta ctccatgagt cggctggcca ccagggtctc ctgccagatc cagtcagggg
      121 ggcggcgatc ctcggcgagc aggtgggcca gggcgacgga cgtgtccagg taaatcaccg
      181 ctcgccgcga tcttgctcga ggccttccaa aacgtcgctc agctttaccg ccgggggagg
      241 ggccactggc ggctggccgg tgcgcagaag cggggcgcgg atgtggccgt ttcgaactgc
      301 gtccgccagg gcggcgtctg cgagcaatgg gctgacagtg ggccccggcg ccgtgatctg
      361 cgccacgacg cgatcgcgat cggtcaccag aacggtttcg ccgcccgctg ccaggcgcac
      421 gtactcgctc agcttgttct tgagtacttt gatgcctacg gccttcattt cacaaaagta
      481 gctactagta gctaccaatg caaccgggat tttcgtctgc ccgaaaaaac tactcgagcg
      541 cggctccctg cggaaccggc agcgcttgta cgagtatgta gtctgtcgcc ggaggcgagc
      601 ggcagccgaa gccgcctcta gcctctccac cgctggacgg ggcctttaca ggagcgcgct
      661 gggttcaacc cgccggccgc ggcctgctgc gcgagcgggc gagagcctgt ctcagttccc
      721 ggtgcttggt ctcggtgagc gggtagcgcc acgcgatcag cgcggtgagg aggagcccgg
      781 tggacggccc gaagacgaac agcagcttga gccccagcgt cgagccttcg taagcgcctg
      841 cctcggcagc cggcacgtag ccggtgaggg agagcagccc cagagcaata gccggcccga
      901 ctgacagggc gaccttggtg gccagggacc agacggcgaa gtaccaggcg gtgcggtcct
      961 cgccgctctc cagccggtcg atgtcgatca cgtcggcctt catggagttg gggatcaccc
     1021 agaaagaccc ggcgagaaaa cccgtgatcg ccgtgatggg cagcatccag tagaagtcgc
     1081 cggctcccag cagcaggtag cccagcatga agaaggcgaa cagcaacaga gcgatgcacc
     1141 aggctcgatg cttgccgacg atgccggagt agcgcacgaa aaacgggatg ccgcagaggt
     1201 tgaacccgta gtacaccagc agctgcagga tggtcttgtc gtcgtcctgc agcacgctgc
     1261 gcacgtagaa gacgatgagc gccgtggaga tggaggtccc cagctggttg acgaagaacg
     1321 cggcgaccag acgtttgaag ggcccattgc gccacatgat cttcagcccc ggcaccaggg
     1381 gcatgcgggc cgggatgaag tccttgcctt cgggaaccat ggccaccgtc agccccacgg
     1441 tcagtggcaa aagaatcagc gtcatggtgc cgacgaggaa cagggtctcc tgggtgcccc
     1501 cgtaggcaca gcagaagatg gccaccacgg gtaccagctt gctgagcacg ttggcggcga
     1561 gcccgatgct ggaccgccag cccgtgatgc gggaacgctc gttgtactcc ggagacagct
     1621 cggccgccca ggcgtagtag ggaatgaaca gcattgtcca gccgagccac agaaccacca
     1681 gccagatcag caggtagacg gcggaggcgt cgccgggatc aggcatgaag agcttgtaga
     1741 tggcgagcat caggataggc acggaagcca gcatccagac ccggcgacgg ccccagcgcg
     1801 tatccgtgcg gtccgacagc cagccgatca gcggatcggt gatggcgtcg aacgatcgcg
     1861 agagcaggag caccagcccg acggccgcca ggctcacgcc cagatcctgg ccgtagtagt
     1921 tgggcacgaa ggcggccacc ggcaccgcgg ccatctggat gggcatgtcg gccaggccgt
     1981 agtagaacag cgtacgccgc gaaagcggcg gcacggcgtc ccggccggct gagtcggtgc
     2041 tagcgtgctt gaccacggac cttctccagg ctggcgcgta tctcccggtg gcgcgactcg
     2101 gtgagcggat gattccaggc aatgagggca gccagccaga agccgcaggc cggcccgaac
     2161 acgtaaagca gcttgagtcc cagcaggcca cccgccgtgg gctccggctg actggccgga
     2221 tcgtagccca cgaaggccag aagcgtcagc gcgagccagg ggccgatgga ttgcccgacc
     2281 ttggtggtaa aggaccagac ggcgaagtac cacgccgcgc ggtcctcgcc ggtgttgagc
     2341 gtgtccacgt cgatcacgtc cgccttcatg gagttcggga gcacccagaa agagccaccg
     2401 agaaacccgg tgagcgc
//
LOCUS       DBNHOX010000391         7631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_159982, whole genome shotgun sequence.
ACCESSION   DBNHOX010000391 DBNHOX010000000
VERSION     DBNHOX010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_159982"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..574)
                     /locus_tag="AC16UZ_08685"
                     /pseudo
     CDS             complement(140..574)
                     /locus_tag="AC16UZ_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(625..1581)
                     /locus_tag="AC16UZ_08690"
     CDS             complement(625..1581)
                     /locus_tag="AC16UZ_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6246798.1"
                     /translation="MQQLPDPVRIPTNDIELEVFEQGAGAPVVLCHGWPEHAYSWRHQ
                     MSPLAEAGYHVIVPNQRGYGHSSRPQKVTDYDITHLAADLTGLLDHFGYDRAVFCGHD
                     WGAIAVWNLAMLHPDRVAGVINLSVPLLRRGPTDWVSHWESLLGEDFYIVHFNRRPGV
                     ADAVFEENPRRFLGNLYRTHQWQEPAAEPRSGMPLINLARANRPRGRPLMSDAELDVF
                     VRAFEHSGFSGGINWYRNFARNWKLTADVPEHVPHPAMMIYGRYDIVPVFDKLRDFVP
                     LVETHVLDCGHWIQQELPGETNRLMLDWLERNYPSNRRRSSQ"
     gene            1658..1873
                     /locus_tag="AC16UZ_08695"
     CDS             1658..1873
                     /locus_tag="AC16UZ_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246799.1"
                     /translation="MRISQLVLKSPIPSYLNRSFYPEGFARQIAGIAIAAAGNRWPAP
                     EQLDVAAFLVHGSDDPLVPVEGGVDTH"
     gene            2048..2443
                     /locus_tag="AC16UZ_08700"
     CDS             2048..2443
                     /locus_tag="AC16UZ_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HIR6246800.1"
                     /translation="MNNTHSLAVGYILWIFGFLGAHRFYFGRPVSGTIYFFTLGLFLI
                     GWLIDLILMPNLEASADRRYQQGEKSYTVAWVLLTFLGFFGVHRFYQGKWMTGIIYLF
                     TVGLFGIGVIYDYWTLNEQVSALNHERAG"
     gene            2519..3283
                     /locus_tag="AC16UZ_08705"
     CDS             2519..3283
                     /locus_tag="AC16UZ_08705"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HIR6246801.1"
                     /translation="MDRVVDLTGAVNFRDFGGYAAADGATVRRGLLFRCGQLASLSQE
                     ALAQFAELGIDVICDLRRPDEREVDPTPVPHHVSRRVEIPMDPGSATQLRDSLRAGYV
                     DVSQRVDFMRTITRELARDHVEGYSSMFAALLEHAPGGFLVHCTAGKDRTGVGVALIL
                     LALGVPRETVIHDYMLTNDVMDFEAFILPRIRQNLGHDNIDVEGARVLSGVREEYIMA
                     ALEEMESACGSLEAYLERAIGLTRGDVDALRAHYLS"
     gene            3365..4690
                     /locus_tag="AC16UZ_08710"
     CDS             3365..4690
                     /locus_tag="AC16UZ_08710"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HIR6246802.1"
                     /translation="MAGTKHQAPRPTGFDTLALHAGARPDPATGARATPIYQSAAFVF
                     PDSDFAAGLFNIERAGHVYSRLSNPTNAVLEERIAALDGGVGAIATASGQAAMHLAVA
                     TLLGAGDHIVASRSLYGGTHNLLAYTLPRFGISTTFVDPRDPQAFADAVREETRLVFG
                     EILGNPGLEVMNVPAVADAAHAAGLPLMIDATFTTPYLFRPLDHGADLVVHSATKFLS
                     GHGVVIGGLIVDGGTFDWEASARFSTLSQPYDGFHGLVFAEEFGPEAFIMRARKEGLR
                     DFGACMAPMTAFQILQGLETLPLRMDRHVANARAIADFLAGHPMVTAVNHPDLAGHPD
                     HDLAKTLLPRGAGAVMGFDIKGGREAGRAFIESLQLFSHLANVGDAKSLVIHPASTTH
                     HRMDAKALEAAGIGEGLIRVSVGLEDPADLIDDLRGALKRAERAAGVST"
     gene            4687..5493
                     /locus_tag="AC16UZ_08715"
     CDS             4687..5493
                     /locus_tag="AC16UZ_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6246803.1"
                     /translation="MRVEVGGHCAFAGTGSVPFDARRPCVVFLHGAGFDHSVWVMPSR
                     YFARHGFAVVAPDFPAHGRSKGAPLASVPALASWLAELLDALGVAEAVVVGHSLGSLV
                     GQAFARLFPDRCLGLALLGTSAPMAVTEQLLGASRDDDHAAIDMANTWSHSPQGRLGA
                     SDNPGIWMLGAEERLLERAAPGVYHADFTACNECDLDQLPGLVSCPVLVIAGGSDSMT
                     PARSGQAVANALSARYVSLPGCGHSMLAERPNQVLNALAEFIGQLPPADH"
     gene            complement(5487..6320)
                     /locus_tag="AC16UZ_08720"
     CDS             complement(5487..6320)
                     /locus_tag="AC16UZ_08720"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246804.1"
                     /translation="MDRLAGRTALITGAARGLGAQIAKRFAEEGAMVIVNDLNLEAAE
                     ATAAAIGGRALACDVSDSSAVNAMFEEVASIADRLDVLVNNAGISGVEGDSNAQLRIQ
                     RAFERADAAARGEVPDLDPPILEVSDEAWGRMLAVHLNGTFYCSRAAVALLNRNTDRP
                     EGGAAIINMGSIMGTSGGAGASAYCAAKAGILGLTRSLARELAPYDIRVNAIAPGYIH
                     TDMTSPMEAMYPLLKAQTPQGRIGEPDDIAWAAVYLASLEAKFVTGQTLSPNGGWHMS
                     Q"
     gene            6468..7160
                     /locus_tag="AC16UZ_08725"
     CDS             6468..7160
                     /locus_tag="AC16UZ_08725"
                     /inference="COORDINATES: protein motif:HMM:NF018949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0090471 - 9,15,9'-tri-cis-zeta-carotene
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0016120 - carotene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HIR6246805.1"
                     /translation="MLQTLLAGLIFVGAHLGISSTSLRPRLVAAVGERAYQGIFSLVA
                     LVTISLLIWTYGEAPRYEYLWLPDPRLYWVTKVVMVVAFISMIGGFMVKNPTAVGMEG
                     ILKDPGQREHAATGMLRITRHPFQWSVVLWSASHIVANGDTVSVVFFAAFGVLSLLGT
                     LAIDRKKAAALGDDWEHFAAVTSNLPFAAVLSGRNRLVLKELVGPGAAGLVVYALVFW
                     GHQWVSGGVRLL"
     gene            complement(7157..>7631)
                     /locus_tag="AC16UZ_08730"
     CDS             complement(7157..>7631)
                     /locus_tag="AC16UZ_08730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6246806.1"
                     /translation="AVLSVLGRALAGAGVGCLRFNFRGVGGSAGAHDGKGGEVEDLLA
                     VERWLRQEHSPAHLILGGYSFGAAVAWQALTSLAPPDQMILIAPPVSFMPSSRRPVGF
                     RLDVFAGDADQFVDAAALNVLGDAGIHIIPGADHFFGGKWGDLAAGIEQVLQPAG"
BASE COUNT         1182 a         2482 c         2585 g         1382 t
ORIGIN      
        1 atcatggaag gcaaagtgat tcgcacggac accgaggagg tggtcgcgga ggccagcggg
       61 agcttcatga tcccgtcctg acgcggcgcc ggcctgcagc ggtctcgagc cgcgtgaacg
      121 gcaagcggca tcgggaaagc tagggccgtc gtgcctccca ctcgtcgaga acctgccggg
      181 cgcgcagccg ccgcccatcc gcgctagcgc cgcttcgcag ctccacgggt gccgccagct
      241 cgaccacgta gccgttcgga tcgcggaagt agatcgagtg aatgaacccg tgatctgaga
      301 tgccgcgcgt ctcgatgccc tgcgcctttc cccgctcgaa catctcgtgg agggtctcca
      361 tgtcgacggt cagtgccaga tgcagatcga agtcgcgctg atccttgaag tcgaacgccg
      421 cgtccggctc ctcgaaaaag gcgacgcagg agccgtcgtc catggcgaag aacgtgtgca
      481 gcacctgggc ggcgcggccg gtcttggtag tgccgatggg cagggcgtcg accaggggca
      541 atcccaggaa atcctcgtag aactgccggg tctcctcaga gttgcggcat cgttaggcgg
      601 cgtggttcaa gcccttgatg gcgttcattg gctgctccgt cgtcggttcg aaggatagtt
      661 tcgctcgagc cagtccagca tcaggcgatt ggtctcgccg ggcagttcct gctggatcca
      721 gtggccgcag tcgagcacgt gggtttccac cagcggaacg aagtccctga gcttgtcgaa
      781 cacgggcacg atgtcgtagc ggccatagat catcatcgcc gggtgcggaa cgtgctcggg
      841 cacgtcagcg gtcagcttcc agttgcgcgc gaagttccgg taccagttga tgccgccgga
      901 gaacccggag tgttcgaagg cacgtacgaa aacgtccagc tcggcgtcgc tcatcagcgg
      961 cctgccgcgc ggccggtttg cccgtgcgag gttgatcagc ggcatgccgg atcggggctc
     1021 ggctgctggc tcctgccatt gatgggtacg gtacaggttg ccgaggaagc gtcgcgggtt
     1081 ttcctcgaag accgcgtctg ccacgccggg ccggcggttg aagtgaacga tgtagaaatc
     1141 ttcgccgagg aggctttccc agtgcgagac ccagtccgta ggcccccggc gcagcagagg
     1201 tacgctcaag ttgatcaccc cggccaccct gtccgggtgc agcatggcca ggttccatac
     1261 cgcgatggcg ccccagtcat ggccgcagaa taccgcccgg tcgtacccga aatgatcgag
     1321 caggccggtg aggtctgcgg cgagatgggt gatgtcgtag tcggtcacct tctgcggtcg
     1381 gctcgagtgc ccgtaaccgc gctggttggg cacgatcacg tggtagccgg cctcagccag
     1441 ggggctcatc tggtgtcgcc aggagtaggc gtgctccggc cacccgtggc acagcaccac
     1501 tggcgcgccc gcgccttgct cgaaaacctc gagctcgatg tcgttggtgg ggatccggac
     1561 tgggtcgggc agctgctgca acgggacgtt cctggtcggg tggagaaaac cctactccga
     1621 gagcgtcccg gcgtgcaatc cgatgttgtg aggcagcttg cgaatctcgc agctggttct
     1681 aaaatcgccg atcccctctt acctaaaccg cagcttctac cccgaaggtt ttgcgcgcca
     1741 gatcgccggc atcgccatcg ccgcggccgg taaccggtgg ccggcgccgg agcagctcga
     1801 cgtcgcggct ttcctcgttc acggcagcga cgaccccctc gtgcccgtgg agggcggcgt
     1861 ggatacccac taggcgctcg agcggtccag actcctggtg ctcgagggca tcgggcacga
     1921 tctgccggag ccggtcgggc ccgaggtcat ccggggcatt gcggagctca cggcacgtca
     1981 ccgttgacgg gcggcgccgg ttcggggcaa cgtgaagcat ctttcgcctg atctgctgga
     2041 ggcccgcatg aacaacacgc acagccttgc ggtcggatac atcctctgga tattcgggtt
     2101 cctgggcgcg caccgcttct atttcggccg gcccgtaagc gggaccatct acttcttcac
     2161 cctgggcctg ttcctcatcg gctggctcat cgacctgatc ctcatgccca acctggaggc
     2221 gtccgccgac cggcgctatc agcagggcga gaagagctac acggtggcct gggtgctgct
     2281 gacgtttctg ggtttcttcg gcgtccaccg cttttatcag ggcaagtgga tgaccgggat
     2341 catctatctc tttaccgtgg gcctgttcgg gatcggcgtg atctacgact actggacgct
     2401 caacgagcag gtgagcgcgc tgaatcacga acgggcgggc tgagcggcgg caggctgcca
     2461 tctcggcttg cgcttctggt aagctcgccg cctcttcacg ctgccacggg aaccttgcat
     2521 ggatagggtc gtcgacctca cgggcgcggt gaactttcgc gatttcggtg gctacgccgc
     2581 cgccgacggc gcgacggttc gtcgtgggtt gctgttccgt tgcggtcagc ttgccagcct
     2641 gagccaggaa gcgctggcgc agttcgcgga gctgggcatc gacgtgatct gcgatctcag
     2701 gcggcccgac gagcgggagg tggatccgac gcccgtgccg catcacgtct cccgccgggt
     2761 cgagattccc atggacccgg gcagcgcgac ccagctgcgg gactcgctgc gggctggtta
     2821 cgtggacgtg agccaacggg tcgacttcat gcgcaccatc acgcgggagc tcgcccgcga
     2881 tcacgtcgag ggttactcgt cgatgtttgc cgcgctgctc gagcatgccc cgggcgggtt
     2941 cctcgtgcac tgcaccgccg gaaaggaccg cacgggggtc ggggtggcgc tgatcctgct
     3001 ggcgctcggc gtgccccggg aaaccgtcat ccacgactac atgctcacca acgatgtgat
     3061 ggatttcgag gcgttcatac tgccgcgcat ccgccagaat ctgggtcacg acaacatcga
     3121 cgtcgaaggg gcccgggtgc tgtcgggggt tcgcgaagag tacatcatgg ccgctctgga
     3181 ggagatggag agcgcctgcg gaagcctgga agcctacctg gagcgcgcca tcgggctcac
     3241 ccgcggtgat gtcgacgcgc tgcgcgcgca ctacctgagc tagggtccgg gcccggagaa
     3301 cggattgcca ctgcccgtgg ggctcggtta gcctcgtcgc cctcctatgc ggaaggcgcc
     3361 catcatggca ggcacaaaac accaggctcc ccgacctacc ggattcgata ctctggcgct
     3421 gcatgccggc gcgcgaccgg atcctgcaac gggcgcccgg gcgacgccca tctatcagag
     3481 cgccgctttc gtcttccccg actcggactt cgccgccggg cttttcaaca tcgagcgcgc
     3541 cgggcacgtt tactcgaggc tttcgaatcc caccaatgcc gtgctggagg agcgcatcgc
     3601 tgcgctggac ggtggcgtag gcgccatcgc cacggccagc ggccaggccg ccatgcatct
     3661 tgcggtggcc acgctgctgg gcgcaggaga tcacatcgtt gcttcgcgat ctctctatgg
     3721 cggcacccac aatcttctgg cgtataccct gccgcgattc ggtatcagca ccaccttcgt
     3781 ggatcctcgc gacccccagg cgtttgctga cgccgtgcgg gaagagaccc gcctggtgtt
     3841 cggtgagatc ctcggcaacc cggggctcga ggtgatgaac gtccccgccg ttgccgacgc
     3901 ggcgcacgca gcggggctcc cgctgatgat cgacgccacg ttcaccaccc cgtacctgtt
     3961 ccgccctctc gatcacggcg cggacctggt tgtgcattcc gcgaccaagt tcttaagcgg
     4021 ccacggagtg gttatcggcg gcctgatcgt cgacggcgga accttcgact gggaggcctc
     4081 ggcgaggttt tcaaccctgt cccaacccta cgacggtttc cacgggctgg tatttgccga
     4141 agagttcggc ccggaagcgt tcatcatgcg cgcccggaag gagggtctga gagatttcgg
     4201 tgcgtgcatg gcgccgatga cggcctttca gatcctccag ggcctcgaaa cgctgccgct
     4261 gcgcatggac cggcacgtgg ccaatgctcg agcaattgcg gattttctag ccgggcatcc
     4321 catggtgacc gccgtgaacc atcccgatct tgccgggcac cccgatcacg acctggcgaa
     4381 gacgctgctg ccgcgcggag cgggtgcggt gatgggattc gacatcaagg gcgggcggga
     4441 agccgggcgc gcgttcatcg aaagcctgca gctcttctcg cacctggcga acgttgggga
     4501 cgcgaagtcg ctggtcatcc atccggccag cacgacccac catcgcatgg acgcaaaggc
     4561 cctcgaggcg gccgggatcg gcgaagggct cattcgggta tcggtcgggc tcgaagatcc
     4621 ggccgacctg atcgacgatc tgcggggggc gttgaagcgg gccgagcggg cggccggagt
     4681 gtccacatga gggtggaagt cggcggtcac tgcgccttcg cgggcacggg cagcgtgccg
     4741 ttcgacgcac ggcggccctg cgtggtgttt cttcacgggg cgggcttcga tcacagcgtc
     4801 tgggtcatgc ccagccgtta cttcgcgcga cacggtttcg cggtggtggc gccggacttt
     4861 cccgcccacg ggcgatcgaa gggggcgccg ttggcttctg taccagccct tgcatcctgg
     4921 cttgccgagc tgctcgacgc gctgggcgtg gcggaggccg tcgtcgtcgg tcacagcctg
     4981 ggctccctgg ttgggcaggc ctttgcccgg ctctttcccg accgctgcct ggggctcgcg
     5041 ctgctgggca cgtccgcgcc catggcggtt accgagcagc tgctgggagc gtcccgggac
     5101 gacgatcacg cagccatcga catggcgaat acctggagtc acagtcctca gggtcggctc
     5161 ggggccagcg acaacccagg catctggatg ctgggcgccg aggagcgtct gctggagcgc
     5221 gcggctcccg gcgtctatca cgccgacttc accgcctgca acgagtgcga tctggatcag
     5281 ctgcccgggc ttgtgagctg ccccgtgctg gtgatcgccg gaggttcaga cagcatgacg
     5341 cccgcgcgct cggggcaggc ggtggccaat gccctttcag cgcgctacgt aagccttcct
     5401 ggctgcggtc actccatgct cgccgagcgg cccaaccagg tgctcaacgc cctggcggag
     5461 ttcatcggcc agctgccccc cgccgatcac tgactcatgt gccacccgcc gttcggcgac
     5521 agggtctgac cggtgacgaa cttcgcctcg aggcttgcca ggtagacggc ggcccaggcg
     5581 atgtcgtctg gctcgccgat gcggccctgg ggcgtctgag ccttgagcag cgggtacatg
     5641 gcttccattg ggctcgtcat gtccgtgtgt atgtatcccg gcgcgatggc gttcaccctg
     5701 atgtcgtaag gcgcaagctc ccgggccagg gaccgggtga gcccgaggat cccggccttg
     5761 gcggcgcagt aggcggaagc cccggcgcca cccgaggtgc ccatgatgct gcccatgttg
     5821 atgatcgcgg cgccgccctc cggccggtct gtgttgcggt tgagcaacgc cacggccgcg
     5881 cggctgcagt agaacgtgcc gttgaggtgg acggcgagca tgcgccccca ggcttcgtcg
     5941 ctcacttcca ggatcggcgg atcgaggtcg ggcacctcac cacgcgcggc ggcgtccgcc
     6001 cgctcgaatg cccgctggat gcgcagctga gcgttgctgt cgccctcgac gccgctgatg
     6061 ccggcgttgt tgacgagaac gtcgaggcgg tcagcaatgg aggcgacctc ctcgaacatc
     6121 gcgttgacgg cggacgagtc gctcacgtca caggccaacg cccgcccccc gatggcggca
     6181 gccgtggctt cggccgcttc gaggttgaga tcgttcacga tcaccatggc gccttcctcc
     6241 gcgaaccgct tggcgatctg cgcgccgaga cccctggcgg cgcccgtgat cagcgccgtc
     6301 ctgcctgcca gtctgtccaa tgtcttccct ccttcccggg tgcccgacga tcgaccttgt
     6361 catcaagtgt ggccgaacac ctcggcgaag cgcaacgagc aggcagcgcg aataagccgt
     6421 ctttcgagta gtatccgtcc gaagctggtc aggaagaccc agagaccatg ttgcagacgc
     6481 tgttggccgg cctgattttc gtgggcgccc atctgggtat cagcagcacg tcgctccggc
     6541 cgcggctggt ggccgcggtg ggcgagcgtg cctaccaggg aatattctct ctcgttgccc
     6601 tcgttacgat ttctctgctg atctggacct acggggaagc tccccgatac gagtacctgt
     6661 ggttgccgga cccgcgcctg tactgggtga ccaaggtggt catggtcgtg gcgttcatct
     6721 ccatgatcgg cgggttcatg gtcaagaatc ccacggccgt gggcatggag ggtatcctga
     6781 aggatcccgg gcagcgcgag cacgcggcca cgggcatgct tcgcatcacc cgccatccgt
     6841 ttcagtggag cgtcgtcctg tggtcggcat cgcacatcgt cgcgaacgga gataccgtgt
     6901 cggtggtgtt cttcgccgcc ttcggcgtcc tgagcctgct ggggaccctg gccatcgacc
     6961 gcaagaaggc ggccgcgctc ggtgacgact gggagcactt tgctgcggtc acctcgaacc
     7021 tgcctttcgc cgcggtgctg tccggccgca accggctcgt gctcaaggag ctggtggggc
     7081 ccggtgccgc ggggctggtg gtctacgccc tggtgttctg gggccaccag tgggtttcgg
     7141 ggggcgtgcg cctgctctag ccggccggct gcagcacctg ttcgatcccg gcggcgagat
     7201 cgccccactt gccaccgaag aagtgatctg cgccggggat gatgtggatc ccggcatctc
     7261 cgagcacgtt cagcgcggcg gcgtcgacga actggtcggc gtcgccggcg aaaacgtcca
     7321 gcctgaagcc gacgggccgg cgggatgaag gcatgaagct cacgggaggg gcgatgagaa
     7381 tcatctggtc gggtggcgcg aggctggtga gcgcctgcca ggcgaccgcg gcaccgaagg
     7441 aatagccgcc caggatcaga tgagcggggc tgtgctcctg gcgcagccag cgctcgacgg
     7501 caagcagatc ctcgacctcg ccgcccttgc cgtcgtgggc gcccgcgctc ccgcctacgc
     7561 cgcggaaatt gaagcgcagg cagcctacgc cggcgcccgc gagcgcgcgg cccaggaccg
     7621 agagaacggc g
//
LOCUS       DBNHOX010000392         5841 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1251368, whole genome shotgun sequence.
ACCESSION   DBNHOX010000392 DBNHOX010000000
VERSION     DBNHOX010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1251368"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="AC16UZ_08735"
     CDS             complement(<1..894)
                     /locus_tag="AC16UZ_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HIR6246807.1"
                     /translation="MSEEEREPKGPGATLREARERMQISTRQVAEALNLPVHTIEAIE
                     AEDPERLPAPVFARGYTRAYARLLELDADAIVAQYPQGEDNVEQVHLIREPRSSASVI
                     RFGLAALLWILGLGVVAVVVWVVWQWSGKDTELAEPVTEARGLLEEVADQPVPSDVGT
                     DPSFSGVPQQEDAALTGREAAEIAGFSAEPEPEPESVAQREVITPRRPGTRPETEPTP
                     SDAGAMATQAAPVEDLAALREAGRRITSAGDDVLRMEFFEDCWVEVMSSTGSLLYGDL
                     NRSGRVLVLVGEAPFRVLLGYA"
     gene            complement(920..1669)
                     /gene="pilW"
                     /locus_tag="AC16UZ_08740"
     CDS             complement(920..1669)
                     /gene="pilW"
                     /locus_tag="AC16UZ_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HIR6246808.1"
                     /translation="MRLGALAILWSVLLSGCVTETTGGLPEPAVAEKRVQAQLDLARG
                     YLENRDYSRARAPLNRALEIDPRSVEGHVLMAVLLQGEGEMALAEQHYKLALRHEPND
                     SQALNNYASFLYRQQRYDEALGLLRELVKDTEYPARSQAYENLGLAELIAGDREAARQ
                     AFIRALNLNFAQPRSSLELARLSYSDGNFELAHEYYEAFRVRARQSPRSLCLGMKISE
                     ALGNSDQTASYGMALQNLYPESAEADECEVP"
     gene            complement(1666..2766)
                     /gene="rlmN"
                     /locus_tag="AC16UZ_08745"
     CDS             complement(1666..2766)
                     /gene="rlmN"
                     /locus_tag="AC16UZ_08745"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR6246809.1"
                     /translation="MEAGLNNLFGYSRAQLEAFVGELDGRPYRARQIMKWAYHQGVVD
                     FSAMTDLSKSFRAALAERSGFWAPEVVARRQSRDGTVKWLVRAENGNCVETVLIPDRG
                     RYTLCVSSQVGCMLDCSFCSTGKQGFNGNLSAADIVGQVWLADRELAQAGQSVTNVVL
                     MGMGEPLLNFDAVVAATSLMMDDLAFGISKRRVTVSTAGVVPGIYELAKVSDVSLAIS
                     LHAPNDELRDVLVPINRKYPIAELLDACRHYLSGLGERRSLTVEYTLLKGVNDQMTHA
                     RQLAMLLQGLKCKINLIPFNPFPASGYERPDARAVRAFQTHLMNSGYATMLRTTRGED
                     INAACGQLVGQVSDRTKRQERYLARVRAQEVA"
     gene            complement(2775..3200)
                     /gene="ndk"
                     /locus_tag="AC16UZ_08750"
     CDS             complement(2775..3200)
                     /gene="ndk"
                     /locus_tag="AC16UZ_08750"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIR6246810.1"
                     /translation="MAVERTLSIVKPDAVARDLIGEIYTRFEKNGLKVVAAKMIRMTQ
                     AQAQGFYAEHDGKPFFADLVAYMTSGPVMVQVLEGDDAVAKNRRLMGATNPKEAEPGT
                     IRADFAESLEANAVHGSDSTTSAAREVAYFFSDEEICVR"
     gene            complement(3305..4468)
                     /locus_tag="AC16UZ_08755"
     CDS             complement(3305..4468)
                     /locus_tag="AC16UZ_08755"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HIR6246811.1"
                     /translation="MRKAVYLDYAATTPVDPRVAQKMSACLLVEGNFGNPASRSHVYG
                     WQAEEAVEHARTQVAKLINADPREIVWTSGATESDNLAIKGVADGAVKRHIVTSAIEH
                     KAVLDTCKYLETKGFEVTYLDPGPDGIVEPGQVHDVLRPDTLIVSIMHVNNEIGVIND
                     IASIGAVCREAGVLMHSDAAQSGGKVAIDVRAMPVDMISLSAHKMYGPKGMGALYVRR
                     EPPLKLTPLIHGGGHERGMRSGTLATHQIVGMGEASAILMRDMERENARITSLRQRLW
                     ERLGKIPGTRLNGDETRRVPGILNVSFPGVDGETLLMALDDVAVSSGSACTSASVEPS
                     YVLRALRVPDELAHSSLRFTVGRYTTEEEVDFAAQRVAEVVAALRGEQSAASQ"
     gene            complement(4468..4926)
                     /locus_tag="AC16UZ_08760"
     CDS             complement(4468..4926)
                     /locus_tag="AC16UZ_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HIR6246812.1"
                     /translation="MRLTTKGRYAVTAMLDIALHRDAGPVSVVDIAHRQDISAAYLEQ
                     LFSKLKRAGLISSIRGPGGGYQLALDAEAINVSRIIAAVGEGVDATRCHGAGDCQDGL
                     TCLTHDLWTELSQEIDSFLGGITLASLVRKRGVRSIASRQDSQLIEARLI"
     gene            complement(4985..5722)
                     /locus_tag="AC16UZ_08765"
     CDS             complement(4985..5722)
                     /locus_tag="AC16UZ_08765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005760054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HIR6246813.1"
                     /translation="MSEVLKGIRIVLVETSHPGNIGGAARAMKTMGLSDLVLVNPKRY
                     PDPQAQWRAAGAQDVLEAARVCADLTEAIEGCYWVVGTSTRSRRIPWPVLSPEEVARQ
                     AVERAGSSIAILFGREANGLSNEELQRCHCHLQIPAAPDYPSLNVAMAVQVVCYELHK
                     LAGEAGDEIGAWDRPTATVAQLDGLIEHLEEVMVASGFLDAANPGQTMTRLRRLFSRM
                     EPDTTEVQILRGILTHLHPLRRPGPGE"
BASE COUNT         1057 a         1959 c         1762 g         1063 t
ORIGIN      
        1 cgcgtacccg agcagcacgc ggaaaggcgc ttctcccacc agcacgagga cgcgtccgga
       61 tcgattgaga tcgccgtaga ggagacttcc agtggaagac atgacttcca cccagcaatc
      121 ctcgaaaaac tccatgcgaa gcacgtcgtc acctgcggac gtgatccgcc gaccagcctc
      181 ccggagcgca gccaggtctt cgaccggggc cgcctgagtc gccatggcgc cggcatctga
      241 cggcgtcggt tcggtttccg gcctcgtccc cggccgccgc ggcgtgatca cctctcgctg
      301 cgccacagac tccggctccg gttcgggctc cgctgaaaac ccggcgattt ccgccgcttc
      361 cctgcccgta agagcggcgt cttcctgctg cgggacgcct gagaatgagg ggtcggtgcc
      421 gacgtcggac ggtaccggct ggtccgcgac ctcttcgagc aggcccctcg cttcggtcac
      481 gggttccgca agctccgtat ctttaccgct ccactgccag acgacccaga cgacgaccgc
      541 gacgacgccc agtcccagaa tccagagcag cgctgcgagg ccgaatcgga ttaccgatgc
      601 gctgctccga ggctcgcgaa tcaagtgaac ctgctcgacg ttgtcttcgc cctggggata
      661 ctgcgccacg atggcgtccg catcgagctc gagaagccgc gcgtaggcac gcgtgtagcc
      721 gcgggcaaag accggcgccg gcaatcgctc cggatcttcg gcctcgatcg cctcgatggt
      781 gtgaacgggc aggttcaggg cttccgccac ctgccgggtg gagatctgca tgcgctcgcg
      841 ggcttcgcga agcgtcgcgc cgggcccctt cggctcgcgc tcttcctcgc tcaagtcgtc
      901 accccggctc ggcccatcat cagggcacct cgcactcgtc cgcttcggca gattcgggat
      961 agaggttctg cagggccatc ccatagctcg ccgtctggtc gctgttgcct agcgcctcgc
     1021 tgatcttcat gcccagacac aggctgcgcg gcgactggcg ggcacgcacc cggaaggcct
     1081 cgtagtattc gtgggcaagc tcgaagttcc catcggaata ggacaaccgg gcaagctcca
     1141 ggctcgagcg gggctgagcg aaattcaggt tcagcgcgcg gatgaacgcc tgccgcgccg
     1201 cctcacggtc tccggctatc aactcggcga ggcccaggtt ttcgtaggcc tgagagcgcg
     1261 ccggatactc tgtgtctttg acgagctcgc gaagcagccc gagcgcttca tcgtaacgct
     1321 gctgacggta gagaaagctc gcatagttgt tcagggcctg agagtcgttc ggctcgtggc
     1381 gcagagcgag cttataatgc tgctcggcca acgccatctc gccctcaccc tgcaagagca
     1441 cggccatcag gacatgacct tccaccgatc gcgggtcgat ctccagcgcg cgattcagcg
     1501 gtgcccgggc tcggctgtaa tcgcggtttt ccaggtagcc acgagccagg tcgagctgcg
     1561 cctgaacgcg tttttcggca accgccggct ctggcagccc acccgtggtt tccgtcacgc
     1621 agccgctgag cagcaccgac cacaagatcg ccaacgcccc tagtctcatg ccacctcctg
     1681 tgcacgcacg cgggccagat aacgctcctg acgtttggtc cggtcgctga cctgaccaac
     1741 cagctgtccg cacgcggcgt tgatatcctc gccgcgagtg gtcctgagca tcgtcgcata
     1801 gcccgaattc atcagatgag tctgaaatgc ccgcacggcg cgagcgtcgg gacgttcata
     1861 tcccgaagcg ggaaacgggt tgaatggaat cagattgatc ttgcacttca gcccttggag
     1921 cagcatagcg agctggcggg cgtgggtcat ctgatcgttg acccccttga gcagcgtgta
     1981 ctccactgtc agagagcgcc gctcgcccaa cccgctgaga tagtggcgac aggcgtcgag
     2041 cagctccgca atgggatatt tccggttgat cggcacgagc acgtcgcgca gctcgtcgtt
     2101 cggcgcgtgc agagagatcg ccagggacac atccgagacc ttcgcgagct cgtagatgcc
     2161 gggaacgacc ccggccgtgc tgaccgtcac ccggcgcttg gatattccga aagcgaggtc
     2221 gtccatcatc agactggtgg cggcgaccac ggcgtcgaag ttgagcagtg gctcgcccat
     2281 ccccatcagc accacgttgg tgacgctttg cccggcctga gcgagctcgc ggtctgcaag
     2341 ccagacctgt ccgacgatgt cggccgcgga cagattcccg ttgaaaccct gcttacccgt
     2401 agagcagaag ctgcagtcga gcatgcagcc gacctgggac gacacgcaca gcgtgtagcg
     2461 gccgcggtcc ggaatcagaa ccgtctccac gcagttgccg ttctcggcac gaaccagcca
     2521 cttgacggtg ccgtcacggg attgccgcct ggcaaccacc tcgggcgccc agaagccgct
     2581 gcgttcggca agcgccgcgc gaaacgactt cgacaggtcg gtcatggctg aaaagtcgac
     2641 cacgccctga tgataggccc acttcattat ctgacgggcg cggtaaggcc tgccgtccaa
     2701 ctctcccacg aaggcctcga gttgggcgcg actgtatccg aaaaggttgt tcagccccgc
     2761 ttccatgtcc agcgctatct cacgcagatt tcctcgtcgc tgaagaagta tgccacctca
     2821 cgggctgcgg aggtggtcga atccgatccg tggacggcgt tggcttccag ggactcggcg
     2881 aaatccgcgc ggatagtgcc gggttccgcc tcctttgggt tcgtcgcgcc catgagccgg
     2941 cgattcttcg ccacggcatc gtcaccttca agcacctgga ccattacggg gccggaggtc
     3001 atgtaggcga cgaggtcggc gaaaaacggc ttgccatcgt gctcggcata aaagccctga
     3061 gcctgcgcct gcgtcatgcg gatcatcttg gccgccacca ctttcagccc gttcttttcg
     3121 aagcgggtgt agatctcgcc aatgagatct cgcgccaccg cgtcgggctt gacgatggat
     3181 aaagtacgct caactgccat caaggagtcc ccttagaatc ttcgaaaagc gcctaggtaa
     3241 ttgaaaaaac gaagaaatga agcgcctcag cgggcgcgta ttatacgcgc cccctgcgca
     3301 gttactactg cgacgccgcg gattgttcgc ccctcaaggc agccaccacc tcggcgaccc
     3361 gctgggcggc gaagtccact tcctcttcgg tcgtgtaccg gccaaccgtg aatcgcagcg
     3421 aggagtgggc gagttcgtct ggcacccgca gcgcccgcag cacgtagctt ggctcgacgc
     3481 tggccgaagt gcacgcggac ccgctcgaga cggccacgtc atccagtgcc atcaaaaggg
     3541 tctcaccatc cacgcccggg aagctcacgt tgaggattcc cggcacccgg cgcgtctcat
     3601 cgccattcag acgggttccc ggaatcttac cgagccgctc ccacaaccgc tgacgcaggg
     3661 acgttatgcg ggcgttctcg cgttccatgt cgcgcatgag aatggcgcta gcttctccca
     3721 tgccgacgat ctggtgggtc gccagggtac cggagcgcat tccgcgctcg tgaccgccgc
     3781 catgaatcag cggcgtcagc ttcagcggcg gctcgcgacg cacgtagagc gcacccatcc
     3841 ctttggggcc gtacatcttg tgcgccgaca gggaaatcat gtccaccggc atggcgcgaa
     3901 cgtcgatcgc aaccttgccg ccgctctggg cggcatcgct gtgcatgagc acgcccgcct
     3961 cccgacagac cgcaccgatc gaagcaatgt cattgatcac gccgatctcg ttgttgacgt
     4021 gcatgatgct gacgatgagc gtgtcgggac gcagcacatc gtgcacctgc cccggctcga
     4081 cgatgccatc tgggcccgga tccagatacg tcacctcgaa acccttggtc tcgagatatt
     4141 tgcaggtgtc cagcacggcc ttgtgctcga tggcgctggt cacgatgtgc cgtttcaccg
     4201 cgccgtcggc aactcctttg attgccaggt tgtcggactc cgtcgcgccg gaggtccaca
     4261 cgatttcccg cggatcggcg ttgatgagct ttgcgacctg ggtcctggca tgctccaccg
     4321 cctcctcggc ctgccagccg tagacgtggg agcgagacgc cggattgccg aaatttccct
     4381 ccaccagcag gcaggcactc attttctggg cgacccgcgg atccaccggg gtggtcgcgg
     4441 cgtaatccag atagacggcc ttgcgcatca gatcagccgc gcttcgatca gctggctgtc
     4501 ctgacgcgaa gcgatgctgc gcacacctcg tttccgcacc aggctggcca gggtgatgcc
     4561 gccgagaaac gagtcgatct cctgagaaag ctccgtccaa agatcgtgcg tcaggcaggt
     4621 caacccatcc tgacagtccc ccgcgccgtg acagcgtgtg gcgtccacgc cttcccccac
     4681 cgccgcgatg atccgggaca cgttgatcgc ttcggcgtcg agagcgagct gatacccgcc
     4741 gcccggaccg cgaatgctgg agatgagccc ggcgcgctta agcttgctga acagctgctc
     4801 gagataggcg gcggagatat cctgacgatg agcgatgtcc acgacgctca ccgggccggc
     4861 atcgcgatgc agcgcaatgt ccagcatagc cgtgaccgca tatctgcctt ttgtcgtcag
     4921 ccgcatggat ggcccaccat cgatacaagc caggcagtat gcaatacccg actaaaatgg
     4981 tccactattc cccggggccg ggacgccgca agggatgcag gtgggtaagg atcccgcgca
     5041 gtatctggac ctccgtcgta tccggttcca tcctggagaa aagccggcgc aggcgggtca
     5101 tggtctgtcc cggattcgcg gcgtccagaa agccgctggc gaccatcacc tcttcgagat
     5161 gctcgatcag cccgtcgagc tgagcaaccg tcgcggtggg gcgatcccag gcgccgattt
     5221 cgtctccggc ctcgccggcc agcttgtgca gctcgtagca caccacctgc accgccatgg
     5281 cgacgttgag cgaggggtaa tcgggggccg cgggaatctg caggtgacag tgacatcgct
     5341 gcagctcctc gttgctgagc ccattggcct cccggccgaa caggatggca atggagctgc
     5401 ccgcccgctc gaccgcctgc ctggcgacct cctccgggga cagcacgggc cagggaatgc
     5461 gccggctgcg ggtgctcgtg ccaaccaccc agtagcaccc ctcgatagcc tcggtgagat
     5521 cggcgcagac acgagcggcc tccagcacgt cctgggcacc tgccgcgcgc cactgcgcct
     5581 gcggatcagg gtagcgcttc gggttgacga gaacgaggtc ggaaagaccc atggtcttca
     5641 tcgcccgcgc agcgccgccg atgttgcccg gatgactcgt ctccacgagg acgatgcgga
     5701 ttcccttcaa tacctcagac acaatcggct tccagtgctc aacagcaatc ggccattttc
     5761 cgacattccc tacctgcaag cacgttgtta ggatagccgc cctcctcttt ggtaccgctt
     5821 tcaacaccat gcaggcaatg g
//
LOCUS       DBNHOX010000393         2543 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1316825, whole genome shotgun sequence.
ACCESSION   DBNHOX010000393 DBNHOX010000000
VERSION     DBNHOX010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1316825"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..865
                     /locus_tag="AC16UZ_08770"
     CDS             74..865
                     /locus_tag="AC16UZ_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR6246814.1"
                     /translation="MARLISLASGTLPEFGPAEVTRAAAAGGFNGCGIWFDAATWTDE
                     TTRGVAAAFADTGLTPLEIEVIDIRPGAPDPEHQRLLESGAAVGATEAIVVSSDPDEV
                     AVCRRFAHLCELGEQLGVNVCLEFLPIYAVRDLPCALRIIRDVGHPRGKLLIDPLHVA
                     RSGASVADIAAVPGELLSFAQFCDGAKEPPGEPTLDVLREEAVNGRFTPGAGQLPLRD
                     LLDVLPDDLPLSLELRSRPLRERFPDPAERARHVYRETARFLGGA"
     gene            935..1969
                     /locus_tag="AC16UZ_08775"
     CDS             935..1969
                     /locus_tag="AC16UZ_08775"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR6246815.1"
                     /translation="MQVGLVTGKETVDLVEMPDPRPEPGKAVVDVSYCGICGTDVHAY
                     QSGEPYNPAICGHEWMGHVSAAGAGVQVKEGDRVAIGMATACGQCATCVRGDPSHCEM
                     AFAGMVGIGPMAAPHGGFASAIAIEAARLYHVGPEISDEAAAILEPVTIAVHAVRRTG
                     IRLGDSVVVVGAGPIGLLVLQCARAAGAGVCVLVEPQATRRETGAALGADHTIDPRET
                     PDVAAAINSIIGKSGADVVFECAGLARTVEEVPAYVRRGGTVALVGYPTGLSQISAAQ
                     WLAQELKVSTALGYVRDDFEVSKGLVADGRVDCGPLHTSTVSLAGMAGAFAELASEPE
                     QIKILVDPRA"
     gene            complement(1992..>2543)
                     /locus_tag="AC16UZ_08780"
     CDS             complement(1992..>2543)
                     /locus_tag="AC16UZ_08780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR6246816.1"
                     /translation="RDQAAILAEGGVDFIALEMMQDVNHAPRAMRAAVATGLPVWLGV
                     SCRRSTQNASLVAFDFADTPFEAPLDALIAMRPAVVNVMHSEVEATVPAIEAVRERWP
                     GPIGAYPEIGYFAAPDRRFGDDHPPDRLVSEARRWVAAGARLIGGCCGTTPDHIRALH
                     AALPELERLAGETETPGRSPTVR"
BASE COUNT          383 a          864 c          875 g          421 t
ORIGIN      
        1 ggggtagcgg gcctgctcgc cagaacgctg caaatgaagc acactggccc aggcagcaat
       61 caggacgatc atcgtggcgc gtctcatatc tctagcatcg ggaaccctgc ccgagttcgg
      121 tcctgccgag gtcacccggg cggctgccgc aggggggttc aacggctgcg gtatctggtt
      181 cgacgccgcc acgtggaccg atgagaccac gcgcggcgtg gcggccgcct ttgcggacac
      241 cggtctgacg ccgctggaaa tcgaggtcat cgacatccgg ccgggagcac cggacccgga
      301 acatcagcgt ttgctggaga gcggcgctgc ggttggggcc accgaggcca tcgtggtctc
      361 gagcgatccc gacgaggtgg ccgtctgccg ccgattcgcc catctctgcg agctcggcga
      421 gcagctgggc gtcaacgtct gcctcgaatt tctgccgatc tacgcggttc gggatctgcc
      481 ttgcgccctg cggatcatcc gggacgtggg acatccgcgc ggcaagctcc tcattgatcc
      541 tctgcacgtc gccaggtcgg gcgccagcgt tgcggacatc gccgctgtgc ccggcgagct
      601 cctcagcttt gctcagttct gcgatggggc caaagagcct ccaggcgagc ctaccctgga
      661 cgttcttcgg gaggaggccg tcaacggccg gttcaccccg ggggccggtc aactgcccct
      721 gcgcgatctt ctggacgtcc tgcccgacga cctgccactc agcctggagc tgcgctcccg
      781 acccctgcgc gagcgtttcc cggatcccgc ggagcgcgcc cgacacgtat atcgcgaaac
      841 ggcccggttc ctcggcggcg cctgaaccgt ccaaggatgc cgctgcgcgc cgactggcgt
      901 atcctttggc gcaattcgaa acgggggtaa ggccatgcag gttgggttag ttacgggtaa
      961 ggaaacggtc gatctcgtgg agatgcccga tccccgcccg gagccgggca aggcggtggt
     1021 ggacgtttcc tattgcggca tctgcgggac ggacgttcat gcctatcagt cgggcgagcc
     1081 ttacaacccg gccatctgcg ggcacgagtg gatgggccat gtctcggcgg caggcgccgg
     1141 cgtgcaggtc aaggaaggcg atcgcgtggc catcggcatg gcaaccgcct gtggccagtg
     1201 cgccacctgc gtgcgcggag acccgtccca ctgcgagatg gcctttgccg gcatggtcgg
     1261 aatcggccca atggcggccc cccacggggg cttcgcctcg gcgattgcca tcgaggcggc
     1321 tcgtctctac cacgtaggcc cggaaataag cgacgaggcc gctgccattc tcgagccggt
     1381 caccattgcc gttcatgcgg tccgccgcac cggtatccgg ctcggcgaca gcgtggtggt
     1441 ggtgggtgcc ggccccatcg gcctgctggt gctgcagtgt gcccgggccg ccggcgcggg
     1501 tgtctgcgtg ctggtggaac cccaggctac ccggcgcgaa accggggcag cgctgggcgc
     1561 cgaccacacc atcgatcccc gggagacccc cgacgtggcg gctgccatca actcgatcat
     1621 cggcaagtcg ggcgccgacg tcgtgttcga gtgtgcgggg ctggcgcgca ccgtggagga
     1681 agtccccgcc tacgttcgcc ggggcggcac cgtggcgctg gtgggttacc ccaccgggtt
     1741 gtcgcagatt tccgcggcgc aatggctcgc acaggagctc aaggtatcca cggcgctcgg
     1801 ctacgtgcgg gacgacttcg aggtttccaa gggcctcgtc gcggacggcc gcgtcgactg
     1861 cggtcccctg catacctcga cggtcagcct cgcaggcatg gcgggagcct ttgccgagct
     1921 cgccagcgag ccggagcaga tcaagattct ggtcgacccc cgcgcctgac cgttctgcgt
     1981 tggagagacc atcaccggac ggtgggcgaa cggcccggcg tctcggtctc cccggcgagc
     2041 ctttcgagct caggcagcgc agcgtgcaac gcccggatgt gatccggtgt ggttccgcag
     2101 cagccgccaa tcaatcgagc gcccgccgcc acccacctgc gagcctcgga caccagacgg
     2161 tccggcgggt gatcgtcgcc gaagcgccgg tcgggggccg cgaagtagcc gatctccgga
     2221 taggcgccga tggggccggg ccatctttcg cggaccgcct cgatggcggg cacggtcgcc
     2281 tcgacctcgc tgtgcatgac gttgaccacc gcaggacgca tggcgatcag cgcgtccagc
     2341 ggcgcttcga acggcgtgtc agcgaagtcg aaagccacga ggctcgcgtt ttgcgtcgat
     2401 ctccggcagc tgacgcccaa ccagacggga agcccggttg ccaccgcggc gcgcatcgct
     2461 cttggcgcgt ggttcacgtc ctgcatcatc tccagggcga tgaaatcgac gccgccctcc
     2521 gccaggatgg cagcctgatc gcg
//
LOCUS       DBNHOX010000394         9847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_95578, whole genome shotgun sequence.
ACCESSION   DBNHOX010000394 DBNHOX010000000
VERSION     DBNHOX010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9847
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_95578"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="AC16UZ_08785"
     CDS             <1..727
                     /locus_tag="AC16UZ_08785"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348656.1"
                     /note="catalyzes the formation of 3-phospho-D-glyceroyl
                     phosphate from D-glyceraldehyde 3-phosphate; involved in
                     growth under gluconeogenic conditions and in glycolytic
                     activity at high ATP concentrations in Corynebacterium;
                     NAD and NADP dependent; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR6246817.1"
                     /translation="LHLKSKGASKVVLTAPGKGSIKNVVSGINSSIIEPTDTILAAAS
                     CTTNAIVPPLKAMNDQFGIVHGHMETVHAYTNDQNLIDNFHKKNRRGRSAALNMVITE
                     TGASTAVTKLLPELDGKLTGNAIRVPTPNVSLAILNLTLSRDTSVAEVNEHLRNVSLH
                     SSMQRQVDFTEAPDVVSSDFVGSRHACVIDGGATLVKDNRAVLYVWYDNEFGYACQVV
                     RVVQKWAGISYPLIPKDVIKQGF"
     gene            926..2269
                     /locus_tag="AC16UZ_08790"
     CDS             926..2269
                     /locus_tag="AC16UZ_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HIR6246818.1"
                     /translation="MIKIKRGLNLPISGSPEQTIETARPARSVALVGGDYPGMRPTML
                     VREGDEVRCGQPLFEDKKNPGVCFTAPASGRVASINRGAKRALQSVVIDVGSAPALEF
                     TAYGPDGMAGLTRSQVVDNLVASGLWTALRTRPFSKVPSPDSEPHSLFVTAIDTNPLA
                     ADPGVVIPEYATEFAAGLDVLAHLTSGAVHVCQESGAFLPAGNDPKLSVQEFAGIHPA
                     GLPGTHIHFLDPVGPSKTVWFIGYQDVAAVGRLFLTGEIMIDRVVALGGPGVEKPRLL
                     KTVLGVNLEELTAGELAEGDQRIISGSVLSGRACAGPVGYLGRYHLQVSVVPEDKERR
                     LFGYLTPGLKKHSVFPIYLSKWLGGADVEFSTTTNGSPRGMVPIGTFESVMPLDVLPT
                     QLLRALLVGDLETAISLGCLELDEDDIALCTYVCPGKYEYGPVLREVLTQIEKEG"
     gene            2270..3454
                     /locus_tag="AC16UZ_08795"
     CDS             2270..3454
                     /locus_tag="AC16UZ_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HIR6246819.1"
                     /translation="MSLRSFLDDVAPHFEKGGRFAWAFPAYEAVDTALYTPGRVTGGP
                     SHVRDGLDLKRIMITVWLCAFIPVFAGSYVVGLQANEVMQSLGIESVDNWRGLFLDNL
                     TSYDPTSIWDCVLHGLAYFLPLYVTVFVAGIVFEIWFASVRGHEVNEGFFVTSILFTL
                     TLPATTPLWMAAVGIVFGVVIGKEVFGGTGKNFLNPALTGRAFLYFAYPAQMSGDAVW
                     VAVDGYTRATALGNAALGESYAVDWLAAFLGNMAGSLGETSTLAILLAGVILVASRIA
                     SWRIMAGVMAGMVAMSVVLNLIESNNPMFAMPFYWHFVLGGFAFGMVFMATDPVSAAM
                     TNAGRWIYGGLIGVMCVLIRVVNPAFPEGMMLAILFGNLFAPLIDHFVVQRNVNRRLA
                     RA"
     gene            3447..4247
                     /locus_tag="AC16UZ_08800"
     CDS             3447..4247
                     /locus_tag="AC16UZ_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HIR6246820.1"
                     /translation="MPDFDKEGLRNVLLVAVSVCLVCSVIVSSAAVILKPRQLINQEL
                     DEKQNILRAAGMLPQGSNQDMQGRGIDELFDQFTVHAVDLETGQFAELDEIDAYDPIK
                     AAKQASRSRELSADEDIATIGRRENVSLVYLRRDSDGVLDKVVIPVRGYGLWGTLYGY
                     LALEGDLNTVAGLGFYSQKETPGLGGEVDNPSWKGQWPGVELFNAAGQPAVRLVKSRS
                     PSASAAAVHEVDALAGATLTTRGVENLVRFWTGELGFGPFLASLKANS"
     gene            4265..4927
                     /locus_tag="AC16UZ_08805"
     CDS             4265..4927
                     /locus_tag="AC16UZ_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HIR6246821.1"
                     /translation="MATTKEVLVDPIFNNNPIALQVLGICSALAVTVSMSTTLVMCAA
                     VVFVTTMSNVAVSLVRNRVPTNIRIIVQMTIIASLVIVVDEVLKAVAYDISKSLSVFV
                     GLIITNCIVMGRAEAFAMQNGPLMSALDGLGNALGYSVILIGVALIREPLGSGTLLGF
                     EILPRVMDGGWYHANGMMLLAPSAFFIIGLFIWAIRSWKKGQVEESEYEIAPNVQYGE
                     AI"
     gene            4930..5538
                     /gene="nqrE"
                     /locus_tag="AC16UZ_08810"
     CDS             4930..5538
                     /gene="nqrE"
                     /locus_tag="AC16UZ_08810"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HIR6246822.1"
                     /translation="MEQYLGLFVRAVFVENMALTFFLGMCTFIAISKKIATAIGLGVA
                     VIVVLAITTPVNNLIFNYLLRDGALAWAGFPDVDLSFLGFLSYIGVIAAMVQILEMFL
                     DRFVPVLYNALGVFLPLITVNCAILGASLFMVERDYTFAESVVFGLGAGVGWALAITA
                     LAGVREKLKYSDVPEGLRGLGITFISVGLISLCFMSFGGIDI"
     gene            5549..6778
                     /gene="nqrF"
                     /locus_tag="AC16UZ_08815"
     CDS             5549..6778
                     /gene="nqrF"
                     /locus_tag="AC16UZ_08815"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HIR6246823.1"
                     /translation="MLDTTIILGVVMFTVVVLFLVMVILFARSRLVSTGEITIEINED
                     PENTIVVPAGGKLLSTLASKGIFLASACGGGGTCAQCRCQVLDGGGSILSTEEGHFTR
                     GEIRDHFRLSCQVAVKQDMKIHIPEDAIGVQRWECEVTSNPNVATFIKELNLKLPEGA
                     NVDFRAGGYVQLEIPPYEMDYKDIDVEDEYQEDWDRFDVWKYHSKVTEPTIRAYSMAN
                     YPEEKGILKFNIRIASPPPGMDVPPGNMSSYVFGLKPGDKLTVFGPYGDFFVKDTPAE
                     KIWIGGGAGMAPLRSQIFDELKRRKQSYTMSYWYGARSLREMFYTEEFDDLASEYDNF
                     EWHVALSDPLPEDEWTGHTGFIHQVVYDNYLKDHPNPEDCEYYLCGPPPMVAAVLQML
                     EDLGVEPENILLDDFGG"
     gene            complement(6828..7454)
                     /locus_tag="AC16UZ_08820"
     CDS             complement(6828..7454)
                     /locus_tag="AC16UZ_08820"
                     /inference="COORDINATES: protein motif:HMM:NF022426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HIR6246824.1"
                     /translation="MPRTLATILLAGTAALGGCASLSESQCLAGDWQTVGYRDGSSGQ
                     NRSALLEHQNACVKHGVTPDREAYLAGWDQGILQYCQPRNGFALGERGAAFPTLCPGA
                     VVDPFEAAYHDGRRLYLARAEIRRLEREINAREHRLEHLDEAIASAESRLVEDGLSSA
                     QRRELLEETKHMSREQGEIEVELKELRIDVALEHQRLAQLRHALASAS"
     gene            complement(7593..7994)
                     /locus_tag="AC16UZ_08825"
     CDS             complement(7593..7994)
                     /locus_tag="AC16UZ_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246825.1"
                     /translation="MESRGIPTICMSSAYSITAAVNPPRAVYLDFPLGHTAGKAGDKA
                     LQRRVMIDTLSALDGILAPGTIRELPYEWSSDDAWKDQAMRPKEGSRPSDDRVERHDT
                     PQYQTAEDRDAAEAQLATGQCPGCVWLSETT"
     gene            complement(8034..8522)
                     /locus_tag="AC16UZ_08830"
     CDS             complement(8034..8522)
                     /locus_tag="AC16UZ_08830"
                     /inference="COORDINATES: protein motif:HMM:NF019003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HIR6246826.1"
                     /translation="MSRPYVDYIQRTREQYDGLGYPPYQWVSNDDEPPFQPLAKPLAD
                     CRVGLIASGGIYRHGQVAFHFRDDLSFREIPRDTEVSELRATHFAYDLTDARKDPNVV
                     FPMETLRDLARTGRIRELSANAYTFMGGIYSARKVRDILAPAIASRLQKDEVDVAILV
                     PV"
     gene            8619..9473
                     /locus_tag="AC16UZ_08835"
     CDS             8619..9473
                     /locus_tag="AC16UZ_08835"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017905872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246827.1"
                     /translation="MREFEDFRDKVAAITGAASGMGRALAVALAERGCHVAIADVHAD
                     GLDETRRRVQAVAGVRCSTHLVDVADRAAVEAFAADTVREHGGVDLIFNNAGVDVTAT
                     VEEMSYADFEWLVNINFWGVVHGTKAFLPYLRQAGQGHVVNTSSLFGLMSVPTQSAYH
                     AAKFAVKGFTDSLRLELADTDIGVSCVMPGGVRTNIVRNARFRISDNQAPTQDEVVER
                     FESRAGLSSEQAAEWILRGVSRNKARILVGRDAQIAAFLLRVFPVSYLKFMTWMRRRN
                     ERVDRRND"
     gene            9490..>9847
                     /locus_tag="AC16UZ_08840"
     CDS             9490..>9847
                     /locus_tag="AC16UZ_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6246828.1"
                     /translation="MSDEVVLYEESENVGVVTLNRPEAMNSLTYQSYLELEDRVRECS
                     ARVLVITGQGRAFCAGDDVKHILGSSQPAPPEFAERARRSGGLTPAADALLHTNIPVI
                     AAVNGPAVGWGMELALM"
BASE COUNT         1735 a         3015 c         3157 g         1940 t
ORIGIN      
        1 cctgcacctg aaatccaagg gcgcgtcgaa ggtcgtcctc acggcgccgg gcaagggcag
       61 catcaaaaac gtcgtctccg ggatcaactc gtccatcatc gagcccacgg acaccatact
      121 ggctgccgcc tcctgcacga ccaacgcgat cgtcccgcct ctgaaagcca tgaacgatca
      181 attcgggatc gttcacgggc acatggagac cgtgcacgct tacacgaacg accagaacct
      241 gatcgataac tttcacaaga agaaccggcg tggtcgcagc gccgctctga acatggtgat
      301 caccgagacg ggagcgtcca cggcggtgac caagcttctg cccgagctcg acggcaagct
      361 gaccggtaac gccattcggg ttccgactcc gaacgtttct ctggccatcc tgaacctgac
      421 gctgtcccgc gacacctcgg tggcagaggt caacgagcac ctgcgcaacg tctcgctgca
      481 ttcttcaatg cagcgccagg tggactttac cgaagcgccc gacgtcgtct cgtcggactt
      541 cgttggcagt cggcacgcct gcgtgatcga cggcggcgcc accttggtca aggacaatcg
      601 ggccgtgctg tacgtctggt acgacaacga gttcggatac gcctgccagg tggtgcgtgt
      661 ggtccagaag tgggcgggta tttcctaccc cctcataccc aaggacgtga tcaaacaggg
      721 attctgaagg gaaaattccc ctaagaatgc gtatcaggtc cgcccaggcg ttggcatgcg
      781 gacacgaccc cactataatt cgcgcggcct gccgaggcgt tcctttcggt gcctccaaca
      841 gccgtatttt cccgctgccc tccggtgagt gggccgagcg cagtccgcga aagcgcaaga
      901 accctggcca ttgttggttc ggaacatgat caagattaaa aggggattga accttcccat
      961 ttcaggaagc cccgagcaga ccatagagac cgctcggccc gcccggagcg tggccttggt
     1021 cggtggcgac tacccgggca tgcgccccac catgctggtg cgcgaaggcg acgaggtccg
     1081 ctgcgggcag ccgcttttcg aggacaagaa gaatccgggc gtgtgtttca cggcgccggc
     1141 ctcgggtcgg gtggccagca tcaatcgcgg cgcgaaacgt gcgctgcagt ccgtcgtgat
     1201 cgatgtaggg tccgccccgg ccctcgagtt cactgcttac ggccccgacg gaatggctgg
     1261 gctcacccgc tctcaggtag tggacaatct cgtcgcgagc gggttgtgga cggccctccg
     1321 gaccaggccg ttctccaagg tccccagccc ggattccgag ccccactccc ttttcgtgac
     1381 cgccatcgac accaacccgc tggcggcgga tcccggggtg gtgatccccg agtacgccac
     1441 ggagttcgct gcaggcctcg atgtcctggc gcacctcacc tctggcgccg tgcatgtctg
     1501 tcaggagtcg ggcgcgtttc tgccagccgg caacgaccct aagctcagcg tccaggaatt
     1561 cgcgggcatc catcccgctg gccttcccgg cactcacatt cattttctgg atccggtcgg
     1621 accgtcgaaa accgtctggt tcatcggcta ccaggacgtc gctgcggtgg gcaggctgtt
     1681 cctgaccggt gagatcatga tcgatcgcgt ggtggccctg ggtgggccgg gcgtcgagaa
     1741 gccgcggctg ctcaaaaccg tgctcggcgt aaatctcgag gaactcacgg ccggcgagct
     1801 cgccgagggc gaccagcgca tcatctccgg ttccgtgctt tccgggcggg cctgcgccgg
     1861 tccggtgggc tacctcggtc gttaccacct tcaggtgtcg gtagtaccgg aagacaagga
     1921 gcgccggctt ttcggttacc tcacgccggg tttgaaaaag cactccgttt ttcccatcta
     1981 cctgtcgaag tggctcggcg gcgcagacgt ggaattctcc accaccacga acggcagccc
     2041 gcggggcatg gttcccatcg gtaccttcga atccgtaatg ccgctggacg tgctgccgac
     2101 gcaactgctt cgcgctctgc tcgtaggcga tctggaaacg gccatcagtc tgggctgtct
     2161 ggaactcgac gaggacgaca tcgcgctgtg cacgtacgtg tgtcccggca aatacgagta
     2221 cggtcccgtt cttcgcgaag tgctcacgca gatcgagaag gagggctaga tgagcttgcg
     2281 atctttcctc gacgatgttg ccccacactt cgagaaaggc ggccggttcg cctgggcatt
     2341 tccggcctac gaggccgtcg acacggctct gtatacccct gggagagtga cgggcggccc
     2401 ctcccatgtc cgtgacgggc tggatctcaa gcggatcatg atcacggtgt ggctgtgcgc
     2461 gtttattccg gtttttgccg gtagctacgt cgtggggctg caggccaacg aggtgatgca
     2521 gtccctgggg atcgagtccg tggacaactg gcgcggcctc ttcctcgaca atctgacgag
     2581 ctacgatccg accagcatct gggactgcgt cctgcatggc ctggcgtact tcctgcctct
     2641 gtacgtgacc gtcttcgtgg cgggaatcgt gttcgagatc tggttcgcct ccgttcgcgg
     2701 gcacgaggtc aacgaaggct tcttcgtcac gagcatactg ttcaccctga cgttgcccgc
     2761 cacgacgccg ctatggatgg ccgccgtcgg gatcgttttc ggagtggtca tcggcaagga
     2821 ggtattcggt ggtaccggca agaacttcct gaatcccgct ctcacggggc gggcgttcct
     2881 ttacttcgcc tacccggcgc agatgtccgg tgatgcggtg tgggttgccg tagacgggta
     2941 cacgcgggcg accgcgctgg gcaacgcggc cctgggagaa tcttacgcag tggactggct
     3001 cgccgccttc ctcggcaaca tggcgggctc cctgggcgag acatcgaccc ttgcgattct
     3061 gcttgccggg gtcattctcg tggcgtcccg aattgcctcc tggcgcatca tggccggcgt
     3121 gatggcggga atggtggcca tgtccgtcgt cttgaacctg atcgaatcga acaaccccat
     3181 gttcgccatg ccgttctact ggcacttcgt gctcgggggc ttcgccttcg gcatggtgtt
     3241 catggccacg gacccggtct ccgccgcgat gaccaatgca gggcggtgga tttacggtgg
     3301 gctcatcggg gtgatgtgcg tgctcatccg cgtggtcaac ccggctttcc cggaaggcat
     3361 gatgctcgcc atcctctttg gcaacctatt tgccccgctc atcgaccatt tcgtagtgca
     3421 gcgcaacgtc aaccggagac tggctcgtgc ctgatttcga caaggaagga ctgcgaaacg
     3481 tgctgctggt ggcggtatcg gtctgcctgg tgtgctcggt gatcgtgtcc tcggcggccg
     3541 tgatcctcaa accccggcag ctgatcaacc aggaactcga cgagaaacag aacatcctgc
     3601 gggccgccgg catgctgccg cagggcagca atcaggatat gcagggccgc ggcatcgacg
     3661 agcttttcga tcagttcacc gtgcatgccg tggatctcga gaccggtcag ttcgctgagc
     3721 tcgacgagat cgacgcatac gaccccatca aggccgcaaa gcaggcgagc cgctcgcgtg
     3781 agctgtccgc agacgaggac atcgcaacca tcggccggcg cgagaacgtc tctctcgtct
     3841 acctgcgtcg agactcggac ggcgtgttgg acaaggtggt gatcccggtt cgcggatacg
     3901 gactgtgggg aacgctttac ggctatctcg ccctggaggg cgacctcaac accgttgccg
     3961 gccttggctt ctactcccag aaggaaacgc cggggctcgg cggcgaagtg gacaacccga
     4021 gctggaaggg gcaatggccg ggcgtggagc tattcaatgc cgccggtcaa ccggccgtgc
     4081 gcctggtcaa gtcgcgctcg ccgtcggcca gcgccgccgc cgtccacgaa gtcgacgctc
     4141 tggccggtgc gaccctgact acccgcggcg tcgagaacct cgtccggttc tggaccggcg
     4201 agctgggctt cggcccattt ctggcaagtc tgaaagcgaa ctcatgaggc gagggtaagg
     4261 aaagatggcg acaacgaaag aagtcctcgt agatccgatc ttcaacaaca atcccattgc
     4321 cctgcaggtg ctcggcatct gctccgcgct tgcggtcacg gtcagcatgt ccaccacgct
     4381 ggtgatgtgc gccgccgtcg tgttcgtgac gaccatgtcc aacgtggccg tgtcgttggt
     4441 gcgaaaccgg gttccgacca acatccggat catcgtgcag atgaccatca tcgcctctct
     4501 ggtgatcgtg gtcgacgagg tgctcaaggc ggtggcttac gacatctcca agagtttgtc
     4561 cgtcttcgtg ggcctgatca tcaccaactg catcgtcatg ggccgagcgg aggcattcgc
     4621 gatgcagaac gggccgctca tgtccgccct cgacgggctg ggcaacgcgc tgggttacag
     4681 cgtcatcctc atcggcgtcg cgctcattcg tgagcctctg ggctcgggaa ctctgctcgg
     4741 gttcgagata ctgccgaggg tgatggacgg cgggtggtat cacgccaacg gcatgatgct
     4801 gctggccccc agcgcatttt tcatcatcgg ccttttcatc tgggccattc gcagctggaa
     4861 aaaggggcag gtggaggaaa gcgagtacga aatcgcgccg aacgttcagt atggggaggc
     4921 catctgatgc tggaacagta tctggggctt ttcgttcgcg cggtcttcgt cgagaacatg
     4981 gccctgacgt tttttctagg catgtgcacg ttcatcgcca tctccaaaaa gatcgcgacg
     5041 gcaatcggcc tcggcgttgc cgtcatcgtg gtgcttgcca tcacgacgcc ggtcaacaac
     5101 ctgatcttca actatcttct gcgtgacgga gcgctggcct gggccggttt ccccgacgtg
     5161 gacttgagct ttctcggctt tctctcgtac atcggcgtga tcgcggccat ggtgcagata
     5221 ctggaaatgt tcctggaccg tttcgtgccc gtgctctaca acgcgctcgg cgtgtttctg
     5281 ccgctgatca cggtgaactg tgccattctc ggagcgtccc tgttcatggt ggagcgcgac
     5341 tacacctttg ccgaaagcgt cgttttcggc cttggggccg gcgtcgggtg ggcgcttgcc
     5401 atcacggcgc tcgccggcgt tcgggagaag ctcaagtaca gcgacgtgcc ggaggggctg
     5461 cgtggtctgg gtatcacatt catctccgtg gggctgatca gtctgtgctt catgtccttc
     5521 ggtggcatcg acatttaagg acggttagat gttagacacg acgatcatcc ttggtgtggt
     5581 catgttcacg gtggttgtcc ttttcctggt catggtgatc ctgttcgccc gctcgcgcct
     5641 ggtcagcacc ggcgagatca ccatagagat caacgaggat cctgagaaca ccatcgtggt
     5701 gccggcgggc ggcaagctgc tcagcaccct ggcctccaag gggattttcc tcgccagcgc
     5761 ctgcggtggc ggcgggacct gcgcccagtg tcgttgccag gttcttgatg gtggcgggag
     5821 catcctctcc accgaggagg gtcacttcac ccgcggcgag attcgtgatc acttccggct
     5881 ttcgtgtcag gtggcggtga agcaggacat gaagattcac attccggagg acgccatcgg
     5941 cgtccagcga tgggagtgtg aagtgacctc gaaccccaac gtggcgacct tcatcaaaga
     6001 gctcaacctg aagctcccgg agggcgccaa cgttgatttc cgagccggcg gttacgtcca
     6061 gctggaaatt ccgccttacg agatggacta caaggacatc gacgtggagg acgagtacca
     6121 ggaggactgg gatcggttcg acgtctggaa gtatcacagc aaggtgaccg aacccaccat
     6181 ccgggcatac tccatggcca attacccgga agagaagggg atcctaaagt tcaacattcg
     6241 tattgcctcg ccgccgcccg ggatggacgt cccgcccggc aatatgtctt cctacgtctt
     6301 cgggctcaag cccggggaca agctcacggt attcgggccc tacggtgact tcttcgtcaa
     6361 agacacgcct gccgagaaga tctggatcgg cgggggcgcc ggcatggcgc cgctgcgctc
     6421 gcagattttc gacgagctca agcggcgcaa gcagagctac acgatgagct actggtacgg
     6481 agcccgatca ctgcgggaga tgttctacac cgaggagttc gacgatcttg ccagcgagta
     6541 cgacaacttc gagtggcacg tcgccctctc ggatccgttg cccgaggacg agtggacggg
     6601 tcatacggga ttcatccatc aggtggtgta cgacaattac ctcaaggacc atcccaatcc
     6661 ggaagattgc gagtactacc tctgcgggcc tccgcccatg gtggcggccg tcctgcagat
     6721 gctcgaggac ctcggcgtcg agccggagaa tattctgctc gacgatttcg ggggttagtc
     6781 gggttttgta gcgcgacgcg aaagcacccg ggcgccgacg tgctggatca gctcgctgat
     6841 gcgagggcgt gacgaagctg cgcgaggcgc tggtgctcga gagcgacgtc gatgcgcagt
     6901 tccttgagct cgacttcgat ctcgccctgc tcgcgggaca tgtgcttggt ttcctccagc
     6961 agttcccgcc gctgcgcgct gctcaaccca tcttccacca gacggctctc agctgacgcg
     7021 atggcctcat cgaggtgctc gaggcgatgc tcgcgggcat tgatctcgcg ctcaagccgc
     7081 cggatttccg cccgcgccag ataaagccgg cgaccgtcgt ggtaggcggc ttcaaacggg
     7141 tcgaccacgg cgcccggaca aagggtcgga aaggcggcgc cgcgctcacc taaagcaaac
     7201 ccgttgcgcg gctggcagta ctgaagaatt ccctggtccc aaccggccag gtaggcttcg
     7261 cgatcggggg tcaccccatg cttgacgcat gcgttctggt gctcgagaag cgccgagcgg
     7321 ttctggccgc ttgacccgtc ccggtagccg acggtctgcc agtcgccggc gaggcactgg
     7381 ctttcgctca ggctggcgca cccacccagg gcggccgtcc cggcgaggag aatggttgct
     7441 agggtccggg gcataggacc tccgttgtca catgcttcct gagggtcagc ggggaaagat
     7501 ggcccaaacg cgcgagccgg accgccaatc agttcacact ttggcagccc gcccggaggt
     7561 gcagggcggc cggaaccggg ccgcggtcgg aatcaggtcg tttcggacaa ccagacgcag
     7621 ccggggcatt ggccggtggc gagctgcgcc tcggcggcgt cccggtcctc ggcggtctga
     7681 tattgaggcg tatcgtgccg ctcgacgcgg tcgtccgaag gccgcgaccc ctccttcggg
     7741 cgcattgcct ggtctttcca ggcgtcgtcg gacgaccact cgtacggcag ctcgcgaatg
     7801 gtgccggggg cgagaatgcc gtccagggcc gacagggtgt cgatcatcac gcggcgctgc
     7861 agggccttgt cgccggcctt tcctgccgtg tgacccagcg gaaagtccag gtacacggct
     7921 ctcggcggat tgacggctgc ggtgatggag taggcgctgg acatgcagat ggtgggaatg
     7981 ccccggcttt ccacctctct tgctatcagc ccgacggact gatggcagac gggtcagacc
     8041 ggcaccagaa tggccacgtc cacctcatcc ttctgcagtc tggaggctat ggccggcgcc
     8101 agaatatcgc ggaccttacg ggccgagtaa atcccgccca tgaacgtgta ggcattggcg
     8161 gagagctccc ttatgcgtcc ggttcgggcg agatcgcgaa gcgtctccat cggaaacacg
     8221 acgttggggt ctttgcgcgc gtcggtgaga tcgtaggcga agtgggtggc tcgcagctcg
     8281 gatacctcgg tatcacgagg gatttccctg aagctcaagt cgtcccggaa gtgaaaggcc
     8341 acctgaccat gccggtagat gccgcctgag gcgatcaggc cgacccggca atctgccagg
     8401 ggctttgcca gcggctggaa ggggggctcg tcgtcgttgc ttacccactg atacggcggg
     8461 tagcccaggc cgtcgtactg ctcccgggtg cgctggatgt aatcgacgta tggtctcgac
     8521 atcgttttct catcatacgg cctttgcccg ccattgcatc ccttttgccg cgatcattaa
     8581 atgcaaaggt gaacgcgaac cacactgcgt cgaggtcaat gcgcgagttt gaggacttca
     8641 gggataaggt ggcggcaatc accggggcgg catccggcat gggacgcgct cttgcggtgg
     8701 cgcttgccga gcgcggctgc cacgtcgcca tcgccgacgt gcacgccgac gggcttgacg
     8761 agacgcgcag gcgggtgcag gccgttgccg gcgtgcgatg ctccacccac ctcgtcgatg
     8821 ttgccgatcg tgctgcggtg gaggcgttcg cagcggatac ggtgcgcgag cacggcggcg
     8881 ttgacttgat attcaacaac gcgggggtag acgtgaccgc caccgtggag gaaatgagct
     8941 acgcagactt cgagtggctc gtgaacatca atttctgggg ggtcgtgcac ggcaccaagg
     9001 cgttccttcc ctacctccgg caggctgggc aagggcacgt ggtcaacacg tcgagcctgt
     9061 tcgggctcat gtccgtgccg acgcagtcgg cctaccatgc cgccaagttc gccgtgaagg
     9121 gctttaccga ctctctgagg ctcgagctgg ctgacacgga catcggcgtg agctgcgtga
     9181 tgcccggagg ggtccggacg aatatcgtgc gcaatgcgag gttccggata tctgacaacc
     9241 aggcgcccac ccaggacgaa gtggtcgagc ggttcgagag ccgggcgggg ctgagctcgg
     9301 agcaggccgc agagtggata ctgcgaggtg tttcgcgcaa caaggctcga atcctcgtgg
     9361 ggcgtgatgc tcagatcgcg gcgttcctgc tcagggtgtt tccggtaagc tatctgaaat
     9421 tcatgacgtg gatgcgacgc cgaaacgagc gggtcgaccg tcgcaacgac tgaacgaggg
     9481 gctgagaaca tgagtgacga ggtagtgctg tacgaggaga gcgaaaacgt cggcgtcgtc
     9541 acgctcaatc gcccggaagc catgaattct cttacctacc agtcttatct cgagctcgag
     9601 gaccgggtgc gcgagtgctc ggcccgggtg ctcgtcatca ccgggcaggg gcgcgcgttc
     9661 tgtgccggtg acgacgtcaa gcacatcctc ggcagcagcc agccggcgcc gcccgagttc
     9721 gcggagcgcg cccgccgcag tggtggtctg acccccgcgg cggacgcgtt gctccacacc
     9781 aacattcccg tcatcgccgc ggtcaacggg ccggccgtgg gctggggcat ggaactcgcg
     9841 ttgatgg
//
LOCUS       DBNHOX010000395         4610 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1188204, whole genome shotgun sequence.
ACCESSION   DBNHOX010000395 DBNHOX010000000
VERSION     DBNHOX010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1188204"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="AC16UZ_08845"
     CDS             <1..381
                     /locus_tag="AC16UZ_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6246829.1"
                     /translation="AERVLRAGARGYVAKEEPPEVVEEALRAVMAGEVYVSKRIAGTI
                     LERLGWADRAAQPSGVDLLSDRELEVFRLVGRGLNSREIAEQVNLGPSTVDSHRARIK
                     KKLGIRNAAELYQRAAQWAAEPGR"
     gene            829..>4610
                     /locus_tag="AC16UZ_08850"
     CDS             829..>4610
                     /locus_tag="AC16UZ_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HIR6246830.1"
                     /translation="MGLSPADAHNLDQRNTSIIFDDETMDQIRQRAQTNQPLVQVGDE
                     LSVVLKSTPGPGTDTGAGGYITFYIPEGTQLAGAEYVRPDPSNPGQFIASPMKGAAIN
                     AIGTGPIGGKATPELIGLTLGPNVLGNSGLAVDAAGLANGTIAGVYADTGIFYSTDPR
                     TAFGSWVRSGGYDGNPATADNLVVNNRGELITPVTQWDGEQLLAYGSKAPAAPIVDYG
                     DGRGNAPWGLANVVAGPESGYAWNFDWSTWKDTAAIPDPAARMKAAVSGVGPWQRIRY
                     PGSQAANDAPGLVSAELGFAGVDASVLGHDLAAAPLPTTNDWTDSTSPKAVRFSVGAL
                     VIDRAEYARVRIKVVSDPGTHATTWDPNGCFIMHTDAFGGDAGGEEGGKDHEWRYYDP
                     TVLTANPCTWVQKVADEPVIGVGGELTYKISIFNLGESALTNVTVTETLPAGVTFVSA
                     FPPQTSGPNPLVWNLGTVLPQTVYEFSITVMPTSAGVVWNDAVVDTDQGEFGSSESVV
                     VGSLAVLELSKSVTPDTVAPGGTVRYSLTIQNDGTGANGLPLIVTDNLPEGFTYAGQL
                     AIELNGAPLSSGFYVADTSDPTQPKFTIKQSIQPGQSAVISFDAVVSSGQDNGTYYNF
                     GWYSDAGMIVGPTPQAPVTVGGGKIGDTIWRDWDGDGVQDAGEEGIEGIVVELYLDAD
                     LDGTFSAGDTLVATDTTDESGNYLFSGLTEGQYVVRVATPPGGYSLTGDPDGTLNGQT
                     SVVLATSESVYRDADFGYAPGGTGSISGFTFNDLNKNAAFGGSDVGIGGATVRLYEDT
                     NGDGLLDAGADLLVGTTTTAADGSYSFSGLDPSLSYLVVVEDDPGSAIDAYFADPYVT
                     TTETPHSVDLAAAGGVYVSANFGYFGVEPASLSGLAYFDADGDGVYGAGDSPLPLVTV
                     SLYLDEDGDGAPGPGELVGTTETDGTGAYSFGDLPPGDYLVVIDASDADIPEGYESTA
                     DTQAVSLNAGDAVPGVDFRFVELLSKSVNASYAEAGQTLTYSLRPYVPGVVSIDNLRI
                     FDPIPSGTTYVPGSANAGGTFGPYAPRAAVPGHDDGPPVLDTAITRDRDFALVGELIT
                     VTLNVRSGVAINDVSPTELRVVGGPIIVVSGPVPLSANVPSGATGVNFVWTVRALEPG
                     AYVFSAGAEDAGGTTTWPDAQSASVLVGVTGGPDVVTWSLGSTTPGSPGETIISGRTA
                     GVFGLAGANKKDFSRYGISNDTWVAKAQPTNGIEKGGALTFDGNDSLYASEGNANI"
BASE COUNT          780 a         1558 c         1539 g          733 t
ORIGIN      
        1 gcggaacggg tgctgcgcgc gggcgcgagg ggctatgtcg cgaaggagga gccgccggag
       61 gtggtggagg aagccctccg cgcggtcatg gcgggcgagg tgtacgtcag caagcgcatc
      121 gccgggacca tcctggagcg gctgggctgg gcggaccggg cggcgcagcc gtcgggggtg
      181 gacctgctgt ccgaccggga gctcgaggtc tttcggctcg tcgggcgcgg cctgaattcg
      241 cgcgagattg ccgagcaggt caacctcggg cccagcacgg tggacagtca ccgcgcgcgc
      301 atcaagaaga agttggggat caggaacgcg gcggaactgt atcagcgcgc ggcgcaatgg
      361 gcggccgagc ccgggcgctg agtccgcgtg tccccttcaa gcctgcgctc ccggccgatt
      421 ccttttcatt acttaatgtt cccgcttcgc tagtagcggt ttttgctaca gcaagagcct
      481 gttgcacgcc acgcgacgcc aaatctaggg tgtcggcagc gtagccaaga gtcggtgaag
      541 cccgtcgctt ggtggccaat gggcatcggg attgaacaca aacaagtgag tactgccgcg
      601 aacctttcgg acaaatcacc gggaccgcgt gacgccaccg cagggtggct tcgccggttg
      661 tggtgtcatt gccgggcccg gagcgggccc cggccgtcca acgcggccgg tgactcgatg
      721 cgatggaagc gccccggtca cggggcttgc agggccttga acgctcccgc aggcgctcat
      781 cgttcgcggc cggtttgggt cgccctcttg gcggtggcgg ggctcctcgt gggcctttcg
      841 ccggccgacg cccacaacct cgaccaacgc aacacgtcca tcatcttcga cgacgagacg
      901 atggaccaga tccgccagcg cgcgcagacc aaccagccct tggtccaggt tggggacgag
      961 ctgtcggtgg tgctgaagag cacccccggc cccggcaccg acaccggcgc cggcggctac
     1021 atcaccttct acattcccga aggcacgcaa ctggccgggg ctgagtacgt ccggcccgac
     1081 ccgtcgaatc ccgggcagtt catcgcttcg ccgatgaagg gcgcggccat caacgcgatc
     1141 ggcaccggcc cgatcggcgg caaggctacc cctgagctca tcggtttgac gctcggaccg
     1201 aacgtgctcg gcaactcggg attggccgtc gatgctgcag gcctggccaa cggcacgatc
     1261 gccggggttt acgcggacac cggaatcttc tactccaccg atccccggac ggcgttcggc
     1321 tcgtgggtca ggagcggcgg ctacgacggc aatcccgcca ccgcagacaa cctggtcgtc
     1381 aacaaccggg gcgaactcat cacgccggtc acccagtggg acggcgagca gctcctcgcg
     1441 tatggatcca aggctccggc tgctcccatc gtcgactacg gcgacggtcg cggcaatgct
     1501 ccgtgggggc tggcgaacgt cgtggccgga ccggaaagcg ggtacgcctg gaacttcgac
     1561 tggtccacct ggaaggacac cgcagcgatc cccgaccccg ccgcccgcat gaaggcggcg
     1621 gtgtccggtg tcggcccgtg gcagcgcatc aggtatcccg gcagccaggc ggccaacgac
     1681 gcgcctggac tggtctctgc agagctcggg ttcgccggag ttgatgcgag tgtcttgggg
     1741 cacgacctgg ctgcggcgcc cctgccaacg accaatgatt ggaccgattc gaccagcccc
     1801 aaggcggtcc gcttctcggt gggcgccctg gtcatcgacc gtgccgaata tgcgcgggtc
     1861 cgcatcaagg tcgtgtcgga ccccgggacc catgcgacga cctgggaccc gaacggctgc
     1921 ttcatcatgc atacggacgc cttcggtggt gacgccggcg gcgaggaggg gggcaaggac
     1981 cacgagtggc gttactacga tcccacggtg ctgacggcca acccctgcac ctgggtccag
     2041 aaggtggcgg atgagccggt catcggcgtc ggcggtgaac tcacctacaa gatctcgatc
     2101 ttcaacctcg gcgagagcgc cctgaccaac gtgacggtca ccgaaacgct gccagcgggc
     2161 gtcacgttcg tcagcgcctt cccgccgcag acgagcggcc ccaatccact ggtctggaac
     2221 ctcggcaccg tcctgccgca gaccgtgtac gagttctcca tcacggtgat gcccacctcg
     2281 gccggcgtcg tctggaacga cgccgtggtc gatacggacc agggggaatt cggctccagc
     2341 gaatcggtcg tcgtcggcag cctcgccgtc ctggagttga gcaagtcggt aacgcccgac
     2401 acggtcgccc ccgggggcac ggttcgctac tcgctgacca tccagaacga cgggaccggc
     2461 gccaacgggc tgccgctgat cgtcaccgac aatctgccgg aggggttcac ctacgcgggc
     2521 cagctggcca tcgagctcaa cggcgctccg ctctcgtcgg gtttctacgt ggcagacacg
     2581 tcggatccga cgcagcccaa gttcaccatc aagcagtcca tacagccggg ccagagtgcc
     2641 gtcatcagct tcgacgccgt cgtcagttca gggcaggaca acggcacgta ctacaacttc
     2701 ggatggtact cggacgccgg catgatcgtc gggccgacgc cgcaggcccc ggtcaccgtc
     2761 ggcggcggca agatcggcga caccatctgg cgcgactggg atggcgacgg ggtccaggac
     2821 gcgggcgagg aagggatcga aggcatcgtt gtcgagctct atctggacgc tgacctcgac
     2881 ggcacgttca gcgccggcga cacgctggtt gccaccgata cgaccgacga gtcgggcaac
     2941 tacctgttca gcggactgac ggaaggccag tacgtcgtgc gggtcgccac tcccccgggc
     3001 ggctactcgc tcaccggcga ccccgacgga accctgaacg gccagaccag cgtggtcctc
     3061 gccacttcgg agagcgtcta ccgggacgcc gatttcggct atgcgcccgg cggcaccggg
     3121 tcgatctccg gcttcacgtt caacgacctg aacaagaacg ccgccttcgg cggaagcgac
     3181 gtgggcatcg gtggggccac ggtaaggctc tacgaggaca ccaatggcga cggcctgctc
     3241 gacgcgggtg ccgacttgct cgtcggcacc accaccaccg cggcggacgg cagctacagt
     3301 ttctcgggcc tcgacccgtc cctgtcctac ctggtcgtcg tcgaggacga cccgggcagc
     3361 gccatcgatg catactttgc cgacccctac gtcacgacca ccgaaacccc gcactccgtc
     3421 gacctcgcgg ccgccggcgg cgtctatgtg tcggcgaact tcggctactt cggcgtcgag
     3481 ccggcgtcgc tgagtggcct ggcctatttc gacgcggacg gcgacggcgt ctacggcgcc
     3541 ggcgactcac cgctgccgct ggtcacggtt tccctctacc tggatgaaga cggcgatggc
     3601 gcgcccgggc ccggcgagct ggtgggcacg accgaaacgg acggaaccgg cgcgtactcc
     3661 ttcggggatc ttccgccggg cgactacctg gtggtgatcg acgccagcga tgccgacatt
     3721 ccggaaggct atgaaagcac cgccgacacc caggcggtgt ccctcaacgc cggcgacgct
     3781 gtccctggcg tcgacttccg gttcgtagag ctcctgagca agtcggtcaa cgcgagttat
     3841 gccgaggccg gtcaaacgct gacctacagc ctgcggccct atgtccccgg ggtcgtctcg
     3901 atagacaacc tgcggatctt cgaccccatt ccctcgggaa ccacctatgt ccccggcagc
     3961 gccaatgcgg gcggcacatt tggcccctat gcgccccggg cggcggttcc cggacacgat
     4021 gacggtcctc ccgtactcga taccgccatc acgcgcgacc gcgatttcgc gctcgtcgga
     4081 gagttgatta cggtgaccct gaatgtcagg tcgggggtcg ccatcaatga cgtttcgccg
     4141 accgagctac gcgttgtcgg tggcccgatc atcgtagtca gtgggccggt gccattgagc
     4201 gccaacgtcc cgtcgggtgc cacgggcgtg aactttgtct ggaccgtacg agcgctcgag
     4261 cccggggcgt acgtgttctc cgcgggcgcc gaggacgccg ggggcacgac cacgtggccc
     4321 gatgcacagt cggccagcgt gctggtcggg gtcacgggtg gccccgacgt ggtcacctgg
     4381 tcgctgggca gcacgacgcc gggcagtccc ggcgagacga tcatcagcgg ccggaccgct
     4441 ggcgtgttcg ggcttgccgg ggccaacaag aaggacttct cgcgctacgg catctccaac
     4501 gacacctggg tcgccaaggc ccagccgacc aacggcatcg aaaagggcgg cgcgctgacc
     4561 ttcgacggaa acgacagcct gtatgcgagc gagggaaatg ccaacatttt
//
LOCUS       DBNHOX010000396         3897 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1510006, whole genome shotgun sequence.
ACCESSION   DBNHOX010000396 DBNHOX010000000
VERSION     DBNHOX010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1510006"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..>3897
                     /locus_tag="AC16UZ_08855"
     CDS             16..>3897
                     /locus_tag="AC16UZ_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246831.1"
                     /translation="MASNGLGASRPLASNETTDGRALNRRVEVEFWYDDPLQDLPDEP
                     QLCPGEPGAETVTRVYDPPWGGIGNLELEGGRPVVPAGYTQALARALGDVADKRNPRL
                     RILGYTRNERLDRRTASVYGDDIGLSAARARRAMETLSAEMGLAPEQAEFEGRGYVHS
                     ADVVNAGFVQDERSYVAVQVVYDDLAVVDDTEGVEVTPLTRELETKNPYALNLMRITV
                     DGEPIDDPNRSSADVQRCTDVAMKGADIRFGFDNLSSKPRLSVSASTAILGVTEAAED
                     SLSASGLQFSMYANYSHFIDRSEIRIFDRDASTQTEPMAVIAVGPDGQATWEPVLDSF
                     AGPVRELKYLLRAYDAQDRFDETTTQPLWLVRETGSAAAAEGPVAEAEEGEPTDPLQP
                     ELLSGYGENALAMHNIRLSSGTVRVSGSEVPEGHRVYVAGREVPVDAKGNFVSEEILP
                     EGAHTVEVAVLDDQGSGESYLRDLAFEPSDWFYVGMADLTFSHNETNGPMELLMGDDA
                     PYDYDSSADGRLAFFVSGKFGNKWRLTASADTREDSLDKLFTNFLSKTPDSLFRRMEP
                     DDHYPTFGDDGTVEELAPTLGKFYVKLSQRENHVMWGNFKVGYMDNELAQIDRGLYGA
                     NFHYQAADTTSFGEQKFSVDAFAAEPGTVASRQEFRGTGGSLYFLRHQDLMVGSERVR
                     VEVRDRSSGLVTGVVNLNPGSDYDVDYLQGSIMLAEPLASTVGDDLLVRSGALGGDEA
                     YLVVRYEYTPGFDEIDALSVGGQMHYWINDHIKVGLTANANEEGGTDSGLHGADLTLR
                     KSADSWVKLQVSQSEGLVTDTLRSSDGGFEFAGYDPSSFSDASAGGYRADVSVGLDDF
                     SSRLDGRITLYAQDLDAGYSGPGLTTVGGARNFGGTFTMPVTRKLEVSAKSDLRKQRQ
                     GVETTAHELDLVYDLTQRWDISAGVRHDELTDRSPVVAPTQDQGQRIDAVMQVGYDSL
                     GRWKAYGFVQDTVSSDGDRPDNARLGLGGQVRVTEKMTIDSEFSTGDLGTGGKLGTTY
                     MHSERTSLYLNYAMDNERTEPMLRAPRGSEGNLVSGIKTRLSDATSVYVESRYRHGDT
                     ANGLTYASGVNFTASNSFNIGANTDIGTLKDLSTGAETDRKAAGVRVAFGTERLQLSS
                     GIEYRSDDTEQLDDTTSNRKTWLFRNTLKYQLNESSRLVGKLNHADSTSSLGNFFAGG
                     YTEAVFGYAYRPVRHDRLNTLMKYTYFYNVPTTDQVSLTQTAADFVQKSHIASVDVTY
                     DLTSRWSIGGKYAYRLGEISYDREDPEFFSNT"
BASE COUNT          721 a         1350 c         1250 g          576 t
ORIGIN      
        1 ctggagacgg cggccgtggc gagcaacggg ctcggcgcat ccaggccgct ggcgtccaac
       61 gagaccacag acgggcgcgc cctgaaccgg cgggtggagg tcgagttctg gtacgacgat
      121 ccgctgcagg atctgccaga cgagccccag ctgtgtccgg gcgagccggg cgcagagacg
      181 gtgacgcggg tatacgatcc gccctggggc ggcatcggca atctcgagct cgaaggcggc
      241 cgtcccgtcg tgcccgccgg ctacacccag gccctggccc gcgccctcgg cgacgtggcc
      301 gacaagcgca acccccgcct gcgcatcctc ggctacacgc gcaacgagcg cctggaccgc
      361 cgcaccgcct cggtgtacgg cgacgacatc ggcctgtccg ctgccagagc gcgacgggcc
      421 atggaaacgc tttcagcgga gatgggcctg gctccagagc aggccgagtt cgaaggccgc
      481 ggctacgtgc actcggcaga cgtggtcaac gccgggttcg tccaggacga acgctcctac
      541 gttgccgtcc aggtcgtcta cgacgatctc gccgtggtcg acgacaccga gggagtggag
      601 gtcacgccgc tcacccggga gctggaaacg aagaacccct atgcgctcaa tctcatgcgt
      661 atcaccgtgg acggcgagcc catcgacgac ccgaaccgaa gctccgccga cgtccagcgc
      721 tgcacggacg ttgccatgaa aggcgccgac atccgcttcg gcttcgacaa cttgagctcg
      781 aagccgcggc tgagcgtgtc cgccagcacc gcgatcctgg gggtcacgga agctgccgaa
      841 gattccctct ccgccagcgg gctgcagttc agcatgtacg cgaactactc ccacttcatc
      901 gaccgctcgg agatccgcat cttcgaccgg gacgcctcca cccagaccga gcccatggcc
      961 gtgatcgccg tgggtccgga cggccaggcg acctgggagc ccgtgttgga ttcgtttgcc
     1021 ggcccggtgc gggagctgaa gtacctgctg cgcgcctacg atgcgcagga ccgcttcgac
     1081 gagaccacca cgcagccctt gtggctggtg cgtgagaccg gcagcgcggc ggctgccgaa
     1141 ggcccggtcg cagaggcaga ggaaggcgag cccaccgacc cgctccagcc ggagttgctc
     1201 tccggctacg gcgagaacgc tctcgccatg cacaacatcc gtctttcgag cggcacggtt
     1261 cgggtcagcg gcagcgaggt tcccgaaggc caccgcgttt acgtcgcggg tcgggaagtg
     1321 cccgtggacg ccaaaggcaa cttcgtcagc gaggaaatcc tgccggaggg cgcccacacc
     1381 gtggaggtgg ccgttctcga cgatcagggc agcggggagt cctatctgcg ggatctggcg
     1441 ttcgagccca gcgactggtt ctacgtgggc atggcggatc tgacgttctc ccacaacgag
     1501 accaacggcc ccatggaact gctcatgggg gacgatgcac cgtacgacta cgactcgtcc
     1561 gccgacggcc ggctggcctt cttcgtcagc gggaagttcg gcaacaagtg gcgcctcacg
     1621 gccagcgccg acacccgtga ggattccctg gacaagctct tcacgaactt cctgagcaag
     1681 acgcccgact ccctcttccg ccgcatggag ccggacgatc actatcccac cttcggggac
     1741 gacggcacgg tggaggagct cgccccgacc ctcggcaagt tctacgtgaa gctcagccag
     1801 cgcgaaaacc acgtcatgtg gggcaatttc aaggtcggct acatggacaa cgagctcgcg
     1861 cagatcgacc gggggctgta cggcgcgaac ttccactacc aggccgccga caccaccagc
     1921 ttcggcgagc agaagttcag cgtggatgct ttcgccgccg agcccggcac ggtggcgagc
     1981 cgccaggagt tccgcggcac cggcgggtcc ctctacttcc tgcgccatca ggatctgatg
     2041 gtgggctccg agcgggttcg ggtcgaggtg cgggaccgat cctccgggct cgtcaccggc
     2101 gtggtgaacc tcaaccccgg ctccgactac gacgtcgact acctccaggg cagcatcatg
     2161 ctggccgagc ccctggcttc cacggtgggc gacgacctgc tcgtgcgcag cggcgcgctg
     2221 ggcggcgacg aggcctatct ggtggttcgc tacgagtaca cgcccggctt cgacgagatc
     2281 gacgccctgt ccgtgggcgg ccagatgcac tactggatca acgaccacat caaggtgggg
     2341 ctcaccgcaa acgccaacga ggaaggcggc acggacagcg ggctgcacgg tgccgacctc
     2401 accctgcgca agagcgcgga ctcctgggtc aagctgcagg tgtcgcagag cgaggggctc
     2461 gtcaccgaca ccctgcgctc gagcgacggc ggcttcgagt tcgccggcta cgacccctcg
     2521 tcgttctccg atgccagcgc cggcggctac cgggccgacg tgagcgttgg cctcgacgac
     2581 ttctcgtccc gcctggacgg ccgaatcacc ctgtacgccc aggatctgga tgccggctac
     2641 tccggccccg gtctgaccac cgttggcggc gcccgcaact tcggcggcac cttcaccatg
     2701 cccgtgacgc gcaagctgga agtcagcgcc aagagcgatc tgagaaagca gcgtcagggc
     2761 gtggagacga cggcccacga gctcgacctg gtatacgacc tgacgcagcg atgggacatc
     2821 agcgccggcg tgcgacacga cgagctcacc gaccgctctc ccgtggtggc gcccacccag
     2881 gatcagggcc agcgcatcga cgccgtgatg caggtgggct acgactccct cggccgctgg
     2941 aaggcctacg gtttcgttca ggatacggtt tcctctgacg gcgacaggcc agacaacgca
     3001 cgcctaggcc tcggcggcca ggtgcgcgtc acggagaaga tgaccatcga ctccgagttt
     3061 tccaccggcg atctcggcac gggcggaaag ctcggcacga cgtacatgca ttcggagcgg
     3121 accagccttt acctgaacta cgccatggac aacgagcgaa ccgagcccat gctgcgcgcg
     3181 ccccggggca gcgagggcaa cctggtttca gggatcaaga cgcggctgtc cgacgccacg
     3241 agcgtgtacg tggagagccg ctaccggcac ggcgatacgg ccaacggcct cacctacgcc
     3301 tccggcgtga acttcaccgc gtccaacagc ttcaacatcg gcgcgaacac ggacatcggc
     3361 acgctgaagg atctgtccac gggagcggag accgaccgca aggccgccgg cgtccgggtc
     3421 gctttcggca ccgagcgcct gcagctctcc agcggcatcg agtaccggag cgacgacacc
     3481 gagcagctgg acgacaccac ctccaaccgc aagacctggc tgttcaggaa cacgctgaag
     3541 taccagctga acgaaagctc ccgtctcgtg ggcaagctga accacgccga cagcacgagc
     3601 tcgctgggca acttcttcgc cggcggctac accgaggccg tgttcggcta tgcgtaccga
     3661 ccggtgcgtc acgacaggct caacacgctg atgaagtaca cgtacttcta caacgtcccg
     3721 accacggatc aggtctcgct cacccagacc gcggcggact tcgtccagaa gagccacatc
     3781 gcgtccgtgg acgtcaccta cgatctcacc tcacgctggt ccatcggcgg caagtacgca
     3841 taccgcctcg gcgagatcag ctacgatcgg gaagaccccg agttcttcag caacacc
//
LOCUS       DBNHOX010000397         2918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_160297, whole genome shotgun sequence.
ACCESSION   DBNHOX010000397 DBNHOX010000000
VERSION     DBNHOX010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_160297"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="AC16UZ_08860"
     CDS             <1..1263
                     /locus_tag="AC16UZ_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6246832.1"
                     /translation="PINTAYTGSEVEYFLRDAEPSLFVCRPSVQPALAEIAGSVPVLT
                     LGADGDGSLLEGALKAEARDEAEPRDPADLAAILYTSGTTGRSKGAMLSQGNLLSNAE
                     SLLECWVWRDDDVLLHALPIFHVHGLFIALHCAFLSGTPVTFLAKFDPEAIVECLPES
                     TVLMGVPTFYTRLLDFPGFGADVCRNMRLFISGSAPLTEATFHAWRERTGFEILERYG
                     MSETIIIASNPLNGERLAGTVGFALPGMEVRIADEEGKPLSGDDVGMIEVRGSSVFSG
                     YWRMPEKTAEEIRPDGFFVTGDLGRMDADGRVTIVGRSKDLVISGGYNVYPKEVERLI
                     DELPGVVECAVIGVPHPDFGEAVVAVVVPENEEVSERSVAAGLDGQLARFKQPKRVIN
                     IGELPRNAMGKVQKNELRERFAHLFAQC"
     gene            complement(1260..>2918)
                     /locus_tag="AC16UZ_08865"
     CDS             complement(1260..>2918)
                     /locus_tag="AC16UZ_08865"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR6246833.1"
                     /translation="SLFLFSMSIIERALEDVSGRAFRRFLRRNTDHPIRGIGSGTLAT
                     AILQSSSLVGVMMLALVGAGVIQMRNALSVIFGANLGTTMTGWIVATIGFKLDLDALA
                     LPLIAAGGLAAMGIRDHRASQVARVVFGVGLLLMGLDLMKGAVGNISDAIDVAVLGEL
                     GAVEYLLFGLVFTAIVQSSSATMMVTLSALHGGIIDLPAAAAVVIGADLGTTGTVLLA
                     ALRGSAAKKRVAMGHFLFNLITDLTAFALRTPLLAVILMIGLSDLLALVAFHSAFNLI
                     GILMFLPLIGRFAGWLEGRFTDTETSVSRHLADVAVDLPEAAVEAIRLETNHLMQRVI
                     CQNLKVPDPEISLPAGSLPVGVHEAGAIAAALEPEFEAAYAASKRLEGEIITFAADAQ
                     AASLEKSESDLITRCQFAAREAVHASKSLKDARGDLRAFALSHRPILNGYGNRFREIM
                     SEFYTRLFLLRHGPTAERADGGPELEDLIVLSQLAHQRHEELHTAIYEDVRADRLDED
                     EISSLLNANRELFNSNRALLSAIATITLPPEQAATFDLLPTSVV"
BASE COUNT          528 a          888 c          974 g          528 t
ORIGIN      
        1 cccatcaaca ccgcctacac cggctcggaa gtggaatact ttctgcgcga tgcggagccc
       61 tcgctcttcg tctgccggcc ctcggtccag ccggcgctgg ccgaaattgc cggcagcgtg
      121 ccggttctga cgctcggagc cgatggagac gggtcgctgc tcgagggcgc gctgaaagct
      181 gaagcgcgtg acgaggcgga gccgcgtgat cctgcagacc tggcggccat tctctacacc
      241 tctggcacca ccggccgctc caaaggtgcg atgctcagcc agggcaacct gctgtccaac
      301 gccgagagcc tgctcgagtg ctgggtctgg cgcgacgacg acgtgctgct ccacgcgctg
      361 cccatctttc acgtgcacgg tctgttcatt gccctccact gcgcgtttct ctccggcacg
      421 ccggtaacct tccttgcgaa gttcgatccg gaagccatcg tcgagtgcct gccggagtcc
      481 acggtgctca tgggggtccc gaccttctat acgaggttgc tggacttccc gggtttcggt
      541 gcggacgtct gccgcaacat gaggctcttc atctccggct ctgcgccgct caccgaagcg
      601 acctttcacg cgtggcgtga gcggacgggt ttcgagattc tcgaacgcta tgggatgagc
      661 gaaaccatca tcatcgcgtc gaacccgctg aatggcgaac ggcttgccgg cacagtgggt
      721 tttgccctgc cgggcatgga ggtgaggatc gccgacgagg agggtaagcc gctgtctgga
      781 gacgacgtgg gtatgataga ggttcgcgga tcgagcgtgt tttcgggtta ctggcgcatg
      841 cccgagaaaa cggcggagga gatccgtccg gacgggtttt tcgttaccgg ggatcttggc
      901 cggatggacg cggacggtcg cgtcacgatc gtgggccgat ccaaggacct cgtcatctcc
      961 ggtggctaca acgtctatcc caaggaggtc gagcgtctga tagacgagct tccaggcgtg
     1021 gtcgagtgcg ccgtcatcgg cgtgccgcat ccagactttg gcgaagccgt ggtcgccgtg
     1081 gtcgttccgg agaacgaaga ggtgagcgag cggtcggtag cggcggggct ggatggccag
     1141 ctcgctcgat tcaagcagcc caaacgcgtg atcaacatcg gcgaacttcc ccgcaacgcc
     1201 atgggcaagg tgcagaagaa cgagctccga gagcgtttcg cccacctctt cgcgcaatgc
     1261 tagaccacgc tggtcggcag caggtcgaac gtggccgcct gctccggcgg cagggtaatg
     1321 gtggcgatgg ccgaaagcag cgcccggttc gagttgaaca gctcacggtt ggcattgagc
     1381 agcgaggaga tctcgtcctc gtccagccgg tccgcccgga cgtcctcgta gattgccgtg
     1441 tgcagctcct cgtggcgttg atgagcgagc tggctgagga cgatcagatc ttcgagctcc
     1501 ggccctccgt ccgcccgttc tgccgtcggc ccgtgacgaa gcaggaagag tcgtgtgtag
     1561 aactcgctca tgatttcacg aaagcggttg ccgtagccgt tcagaatggg ccgatgggag
     1621 agggcgaacg ctctgaggtc gcctctcgcg tccttgagac tcttcgatgc gtggacggct
     1681 tcgcgggcgg caaactggca tcgcgtgatg aggtcgcttt cgctcttctc cagtgatgcc
     1741 gcctgggcgt ccgcggcaaa cgtgatgatc tcgccctcga ggcgtttgct tgccgcatat
     1801 gccgcttcga actcgggctc cagcgcggcg gcgatggcgc cggcttcgtg cactcccacc
     1861 ggtagcgaac ccgcgggcaa cgatatctcg ggatccggca cctttaggtt ctggcagatc
     1921 acccgttgca tgaggtgatt ggtctccagg cggatggcct ctactgcggc ctcgggcaga
     1981 tcgacggcga catcggcgag gtggcggctc accgaggtct cggtgtcggt gaaacgtcct
     2041 tcgagccaac cggcgaaccg accgatcaac ggcaggaaca tcaggattcc gatcagattg
     2101 aacgcgctgt ggaaggcgac cagagcgagc agatctgaca acccgatcat gaggatcacg
     2161 gccagcaacg gcgttcgcag cgcgaaggcc gtgagatcgg tgatcagatt gaacaggaag
     2221 tggcccatgg caacccgttt tttggcggcg ctgcccctca aggccgccag cagcacggta
     2281 cccgtggtgc cgaggtcggc cccgatgacc actgcagctg ccgcagggag gtcgatgatg
     2341 ccgccgtgca gcgcgctcaa ggtcaccatc atggttgccg agctggactg gacgatggcg
     2401 gtgaagacga gcccgaagag gagatactcc accgcgccga gctccccgag aacggcaacg
     2461 tcaatggcat ccgatatgtt gcccacggcg cccttcatca ggtcgaggcc catgagcagc
     2521 agacccaccc cgaagacgac gcgggcgacc tgggatgcgc gatgatcgcg aatgcccatg
     2581 gcagcaagac cgccggcggc gatgaggggc agcgccagcg cgtcgaggtc gagcttgaag
     2641 ccgatggttg ccacgatcca cccggtcatg gtggtgccga gattggcacc gaatatcaca
     2701 gacagggcgt tgcgcatctg gatcacgccg gcgcccacca gggcgagcat catcacgccg
     2761 accagggagc tcgactgaag gatggccgtt gccagcgtgc cgctgccgat gcccctgatg
     2821 ggatggtctg tgttgcggcg gagaaatctg cggaaggctc ggccggagac gtcctctaaa
     2881 gcgcgctcga tgatgctcat ggagaacagg aacaggct
//
LOCUS       DBNHOX010000398         3726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_931614, whole genome shotgun sequence.
ACCESSION   DBNHOX010000398 DBNHOX010000000
VERSION     DBNHOX010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_931614"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1362)
                     /locus_tag="AC16UZ_08870"
     CDS             complement(184..1362)
                     /locus_tag="AC16UZ_08870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6246834.1"
                     /translation="MKFGIFYELQLPKPWEADSEYRLFNNALDQIELADRLGFDYAWE
                     VEHHFLEEYSHSSAPEVFLAAASQRTRNIRLGHGIVQLPTNHPARVAERVSTLDLISH
                     GRVELGLGEGSSVTELHPFGRRFREKRAVWEDAVRATLPMFWEEGWEYHGEFFDFPLR
                     NVIPKPRQQPHPPLWVACSQMDTIRMAGRRGMGALGFSFVSPESAQAWCHAYYNEFVK
                     RQDRLCDYQPNANLAVVSPFMCCETDEEAQEKMDGWSFFQFALILYNKEGPFEPGTVN
                     LWDRYQEWKQSGKGRDLTPNSGLVGSPETIRTRLQQFQDVHIDQVILLNQAGKNRHED
                     ICASLELFAREVMPEFHAAEAGHQEWKAAVLDGSLELEDIDTDQYNIYSLQTPNSKAS
                     "
     gene            complement(1393..1683)
                     /locus_tag="AC16UZ_08875"
                     /pseudo
     CDS             complement(1393..1683)
                     /locus_tag="AC16UZ_08875"
                     /inference="COORDINATES: protein motif:HMM:NF041106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1821..1955)
                     /locus_tag="AC16UZ_08880"
     CDS             complement(1821..1955)
                     /locus_tag="AC16UZ_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246835.1"
                     /translation="MTTGMTVMIVMTTLAVTVGVVALRVSWRIVNRLSEERPQNEEER
                     "
     gene            2081..>3726
                     /locus_tag="AC16UZ_08885"
     CDS             2081..>3726
                     /locus_tag="AC16UZ_08885"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6246836.1"
                     /translation="MYDRATTYGSDRVHEGPCHEHGPHGCPCCAATLGELLRRVGLDV
                     EAQAGRSVPAAPRAPVPTLFCNARVLTMDDAGSEFDTLLISDGRIQWVGNADADAVPE
                     VGDDTVRVDCNGRTILPGFVEPHMHLSAIAMLRRFENVGPFRYPTVSGALARLKEVAA
                     DTDPGAWIIGRQFDPSLQEGPGTLTASMLDEVSTTHPIFVYNASLHFGYCNSLALEIA
                     GITRDTPDPEGSPFGRLPDGTPDGVLKGGVSQAMVARHNPMLREHDLVEGCLDVFRHA
                     NSVGFTTICDQGTGAMQGAKELALYEALRESNRMTARFRYSLFNTLAADWEASDVSFG
                     TGDSWVRASAWKIVSDGSNQGLSGLQREPFMNADSHGIAYIEPEALNEAVKRRLGQGW
                     PVAVHANGDLAIDRVLDAFEAARAAGLDPARARCRIEHCSILHDEQIERIADLGLSPS
                     FLIGHVYWWGKAFRDEIFGLEKASLLDRTAACEAKGVRWTLHSDEPVTEMGPLRCIEN
                     AVTRRLWRTDDEVLAPHECVGVEQALRAMTRDAAWQCHSD"
BASE COUNT          600 a         1224 c         1214 g          688 t
ORIGIN      
        1 gcagctgatt gccaatgggt agcagcgcgt tttccaggcc gaagtgggcg acgccgggat
       61 cgcgatagca gacccgtatg ccaagaaccg actccagctg cgcctcgatg ggctctagct
      121 ttgcagccac cagggcgatc tggcgcagtc gtaaccacat gtttcctccg ttaacggtaa
      181 tgctcaggac gctttgctgt tcggcgtctg aagcgagtag atgttgtact ggtccgtgtc
      241 gatgtcctcc agctccaggg agccgtctag gacggcggcc ttccactcct ggtgcccggc
      301 ttcggcggcg tggaactcgg gcatcacctc cctggcgaag agctccaggc tggcgcagat
      361 gtcctcgtgg cggttcttgc cggcctgatt gagcaggatg acctggtcga tgtgcacgtc
      421 ctgaaactgc tgcagacgtg tccggatggt ttccggtgag cccacgagcc ccgagttcgg
      481 tgtcaggtca cggcctttgc cggattgctt ccactcctgg tatcggtccc acagattcac
      541 ggtgcccggc tcgaagggtc cttccttgtt gtagaggatg agcgcgaact ggaagaacga
      601 ccagccgtcc atcttctcct gggcttcctc gtcggtttcg cagcacatga aaggagagac
      661 cacggccagg ttggcgttgg gctggtagtc gcagagccgg tcctgacgtt tgacgaactc
      721 gttgtagtag gcgtggcacc aggcctgggc ggactccggg ctcacgaacg aaaagcccag
      781 cgcgcccatg cctcggcggc cggccatgcg gatggtgtcc atctgggagc aggcgaccca
      841 cagcggcggg tggggctgct gtcggggctt gggaatcacg ttgcgcaggg gaaagtcgaa
      901 gaactcgccg tgatattccc agccctcctc ccagaacatg ggcagagtgg cccttaccgc
      961 gtcctcccag accgcccgct tttcgcggaa acgacggccg aaggggtgca gctccgtgac
     1021 gctggagcct tcgcccagcc cgagctccac gcggccgtgg ctgatcaggt ccagggtcga
     1081 gacccgctcg gccaccctgg cggggtgatt ggtgggcagc tgcacgatgc cgtggcccag
     1141 gcggatgttg cgcgtgcgct ggctggccgc ggccagaaag acctctggtg cggaggagtg
     1201 cgagtactcc tcgaggaagt ggtgctccac ctcccacgcg tagtcgaagc ccagccggtc
     1261 ggcgagctcg atctgatcca gcgcgttgtt gaacagccgg tactcgctgt ccgcttccca
     1321 gggttttggc agctgcagct cgtagaagat gccgaatttc atcctcgcct ccctctccgg
     1381 ccgctgccgg tcctacccgc cgaacacgcc gccgtaggcc tcctcgttga agccgaccag
     1441 cagcgtcttg cccactttca gcgtaggggc acgcagattg ccggtcggtc cgagcatggc
     1501 cgccaccgcg tcgtcggtgg ccgctttacc accgccgaac tcgctgattt tcttaccctt
     1561 cgcgacgatc attttcgacg ctttgccgag cagctcgcgg gcgtccggtt ccgtcagctt
     1621 ctggctggcc ggcgtgcggt ccttgatctc tattttgctt gcctccaaga acttggatgc
     1681 tctggtacag ctggttcagc cgttgcggta gtaataccag tcgacgcgct ttgccacggt
     1741 cgtccctccg ttgattcaga ctcgttgcgg tatgggggat cgcgccgacg ggcccctgat
     1801 ggccgtcgct gggatagcgg tcagcgctcc tcctcgttct gcggccgctc ttccgacagc
     1861 cggttcacga tacgccacga cacgcgcaga gcgacgacgc cgacggtcac cgccagcgtg
     1921 gtcatgacga tcatcaccgt catcccggta gtcatggtgc ttcctcccga taccgtggtt
     1981 gctacggtgc ctcattgcgc gctccgggtc cagtgttccc aatgtgggct ccatgcaaat
     2041 agcgtaatct atgcgcttgt ggcctcgggg ggagatgcgc gtgtacgaca gggcaactac
     2101 ctacggatca gaccgcgttc acgagggtcc ctgccacgag cacggcccgc acggctgtcc
     2161 ctgctgcgcg gcgactctgg gcgagctcct gcgccgggtg gggctggacg tcgaggccca
     2221 ggcggggcgg tccgtgccgg ccgcaccgag ggcgcccgtg cccacgctgt tctgcaacgc
     2281 cagggttctg accatggacg atgcgggctc ggagttcgac acgctgctga tctccgacgg
     2341 gcgcattcaa tgggtcggca atgccgatgc cgatgccgtg cccgaggtcg gtgacgacac
     2401 cgtgcgcgtg gactgcaacg gcagaaccat cctgccgggg ttcgtcgaac cccacatgca
     2461 cctctccgcc atcgccatgc tcagacggtt cgagaacgtg gggcccttcc gctacccgac
     2521 cgtgagcgga gcgctggcca ggctcaagga agtcgcagcc gataccgacc ctggcgcgtg
     2581 gatcatcggc cgacagttcg acccctcgct gcaggagggg cctggcaccc tgaccgcctc
     2641 catgctggac gaagtcagca caacgcaccc gatcttcgtc tacaacgcgt cgcttcactt
     2701 cggctactgc aactccctcg cgctggagat cgccgggatc acccgggata cgccggaccc
     2761 ggaaggttcg cccttcggac gactgcccga cggcaccccg gacggcgtcc tcaagggcgg
     2821 cgtctcccag gccatggtgg cgcggcacaa ccccatgctg cgcgagcacg atctggtcga
     2881 ggggtgcctg gacgtgttcc gccatgccaa cagcgtcggc ttcaccacca tctgcgatca
     2941 gggcaccggc gcgatgcagg gcgccaagga gctcgctctt tacgaagcgc tgcgcgaaag
     3001 caaccggatg acggcgcgct ttcgttactc cctgttcaat acccttgccg ccgactggga
     3061 ggcctccgac gtctcgttcg gcacgggaga cagctgggtt cgcgccagcg cctggaagat
     3121 cgtcagcgac ggttccaatc aggggttgag cgggctgcag cgggagccgt tcatgaacgc
     3181 cgactcccac ggcatcgcct acatcgaacc cgaggcgctc aacgaggccg tgaaacgaag
     3241 actcgggcag ggctggccgg ttgccgtgca tgccaatggc gatcttgcca tcgacagggt
     3301 gctggatgcc ttcgaggccg cccgggccgc ggggctcgat ccggcgcgag cgcgctgccg
     3361 catagagcac tgcagcatcc tccacgacga gcagatcgag cggatcgccg acctcgggct
     3421 ctcgcccagc tttctgatcg gccacgtcta ctggtggggc aaggcctttc gagacgagat
     3481 attcgggctg gagaaagcca gtctcctgga ccggaccgcg gcctgcgagg ccaagggcgt
     3541 gcgctggacc ctgcactccg acgagcccgt caccgagatg ggtccgctgc gctgcatcga
     3601 gaacgccgtg acccgccggc tgtggcggac cgacgacgag gtgctcgccc cccatgaatg
     3661 cgtgggcgtg gagcaggcgc tgcgggcgat gacccgggat gcggcctggc agtgtcactc
     3721 ggatca
//
LOCUS       DBNHOX010000399         2694 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1381725, whole genome shotgun sequence.
ACCESSION   DBNHOX010000399 DBNHOX010000000
VERSION     DBNHOX010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1381725"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="AC16UZ_08890"
     CDS             <1..350
                     /locus_tag="AC16UZ_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6246837.1"
                     /translation="ARLTAPLWGVYGNNDQERDSLEQAVAAHGFSFTEPPLHLTWHGR
                     KIVVVHDPLEFDGVLNDGHELALHGHTHLYRYEERASQVIFNPGECAGHMAGFNAVGV
                     VDLSDLSAELLRF"
     gene            complement(358..1098)
                     /locus_tag="AC16UZ_08895"
     CDS             complement(358..1098)
                     /locus_tag="AC16UZ_08895"
                     /inference="COORDINATES: protein motif:HMM:NF019032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HIR6246838.1"
                     /translation="MAADVNSWWPRAILIGAVIAAVLLVAGALGTRIGLWDFQVGFLG
                     LAGGTILAAIGVIVGAVGLIASVKRELPQNRPPIYLGLAISVVILIVMGMQFDKAYSV
                     PPIHNISTDLEDPPAFDQVVALRGEESNPLALNAQEIGPMQREAYPWVVPLVTPTARD
                     EMFSRALAVVDDMGFELVNADPAAGIIEATDTTFWFGFKDDVAIRVRSAEAGTVVDVR
                     SVSRVGLSDLGANAKRIGDILAALGAGA"
     gene            complement(1138..1917)
                     /locus_tag="AC16UZ_08900"
     CDS             complement(1138..1917)
                     /locus_tag="AC16UZ_08900"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246839.1"
                     /translation="MASDLVDLTGKVALITGSSKGIGRAIAEEMARAGAQVVISSRKA
                     DLCDEVAAAINAELASEAGGAVAIPAHIGHKEELERLVTQTREKLGRIDVLVCNAAVN
                     PYFGSMTELPDSAFDKILDVNIKSNHWLIQMVLPEMLEREDGSIIVVSSVGGLRGSTH
                     LGAYAISKAADAQLVRNLAAEHGPRNIRVNTISPGLVRTDFARALWENPDILKARTSG
                     DPLRRIGEPREIAGIAVYLASRAGAFTTGQNFVIDGGATIV"
     gene            complement(1921..>2694)
                     /locus_tag="AC16UZ_08905"
     CDS             complement(1921..>2694)
                     /locus_tag="AC16UZ_08905"
                     /inference="COORDINATES: protein motif:HMM:NF048962.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7064 domain-containing protein"
                     /protein_id="HIR6246840.1"
                     /translation="GGMAFNVVEPFKHLTVRYQGKLCVLADPQQMADPANAFKTNPVV
                     ESEIELDFHGISPMFGGEPVNEDGSAIEMKAEESFARGHTEQHTRGAGFIRVGDERFE
                     IDGLGLRDHSWGPRFWQAIYWYRWLPMVFSEDFAMMVSITCRPDAEPRAGGMVLRDNE
                     YVMIRDARIETVYDANDCQETFKVWARTDEREYEVEGKVMSLIPLRNRRRTPDGEDLM
                     TRITEGMTEYRCDGRIGYGLSEYLDQVVDGAPVGVKAGM"
BASE COUNT          434 a          946 c          850 g          464 t
ORIGIN      
        1 tcgcgcggct caccgcgccc ttgtggggcg tctacggaaa caacgatcag gaacgggact
       61 ccctcgagca ggccgtggcg gcccacggct tctcattcac ggagccgccg ctacatctca
      121 cctggcacgg ccggaagatc gtggtggtgc acgaccccct ggagttcgac ggcgttctga
      181 acgacgggca cgagctggcg ctgcacggcc acacccacct gtaccgctac gaggagcgcg
      241 ccagccaggt catcttcaat cccggcgagt gcgcgggcca catggccggg ttcaacgccg
      301 tgggcgtggt tgacctgtcg gatctgagcg ccgagctgct gcgcttctag ccccggttta
      361 cgccccggcc cccagggctg cgaggatgtc tccgatccgc ttggcgtttg cgcccagatc
      421 gctcaatccc acccgggata cgctgcgaac gtccaccacg gtgcccgcct cggccgatcg
      481 cacgcggatg gcgacgtcgt ccttgaagcc gaaccagaag gtggtgtccg tcgcctcgat
      541 gatgcccgcg gcaggatcgg cgttcacgag ctcgaagccc atgtcgtcca ccacggccag
      601 agcccgggaa aacatctcgt cccgcgctgt cggggtcacc aggggcacga cccacggata
      661 ggcttcacgc tgcatgggcc cgatctcctg ggcgttcagc gccagcgggt tggactcctc
      721 gccgcgcagc gcgaccacct ggtcgaaggc cggcgggtct tccagatccg tggagatgtt
      781 gtggatcggc ggcacggagt aggccttgtc gaactgcatg cccatgacga tcagtatcac
      841 aacgctgatc gcgaggccca agtagatggg cggtcggttc tggggaagct cccgcttcac
      901 cgaggcgatg aggcccacgg caccgacgat gacgccgatg gcggccagga tcgttccgcc
      961 cgcgagaccc aggaagccca cctgaaaatc ccacagcccg atgcgggtgc ccagcgcgcc
     1021 cgccaccagc aggacggcgg cgatcaccgc gccgatgagt attgcccgcg gccaccagct
     1081 gttaacgtct gcagccatgt cactcctccc tgaattcggt tacgcgcggg agcataccta
     1141 gacgatggtc gcgccgccat cgatgacgaa attctgcccc gttgtaaaag cgccagccct
     1201 ggacgctagg tacacggcga tgcccgcgat ctcccggggc tcgccgatcc gccgcagcgg
     1261 atccccggac gtgcgggctt tcaggatgtc cggattttcc cagagggccc gggcgaagtc
     1321 cgtgcgcacg agcccgggcg agatggtgtt caccctgatg ttcctcggcc cgtgctcggc
     1381 cgccaggttc ctcaccagct gcgcgtccgc cgccttgctg atggcatagg cgccgaggtg
     1441 ggtgctgccg cgcaatccgc ccacggagga gacgacgatg atggatccgt cctcccgctc
     1501 cagcatctcc ggcaggacca tctggatcag ccagtggttg gacttgatgt tcacgtccag
     1561 gatcttgtcg aaggctgaat ccggcagctc ggtcatggag ccgaagtacg ggttgacggc
     1621 cgcgttgcag accagcacgt cgatcctgcc cagcttctcc cgcgtctggg tcacgagcct
     1681 ctccagctcc tccttatggc cgatgtgggc cggtatggcc accgcgccgc cggcctcact
     1741 ggccagctcc gcgttgatgg ccgcagccac ctcgtcgcaa aggtcggcct tgcggctgga
     1801 gatcaccacc tgggcccccg cccgggccat ctcctcggca atggcccggc cgatgccctt
     1861 gctggacccc gtgatcaggg ccaccttgcc tgtcagatcg actagatcac tcgccatggg
     1921 tcacatcccc gccttgacgc caaccggagc gccgtccacc acctgatcca gatactccga
     1981 aagcccgtag ccgatccggc cgtcgcagcg gtattccgtc atgccctcgg tgatgcgggt
     2041 catgaggtcc tcgccatcgg gcgtgcgccg ccggttgcgc agcgggatca gcgacatgac
     2101 cttgccctcg acctcgtatt cccgctcgtc ggtgcgcgcc cagaccttga aggtctcctg
     2161 gcagtcgttg gcgtcgtaga cggtctcgat gcgggcatcg cggatcatca cgtactcgtt
     2221 gtcccgcagc accatgcccc ccgcccgggg ctctgcgtcc ggccgacagg tgatggagac
     2281 catcatggcg aagtcctcgc tgaacaccat gggcagccag cggtaccagt agatggcctg
     2341 ccagaagcgc ggcccccagg agtggtcccg caggcccagc ccgtcgatct cgaaacgctc
     2401 gtcgcccacc cggatgaagc ccgcgccccg ggtgtgctgc tcggtgtggc cccgggcgaa
     2461 ggattcctcg gccttcatct cgatggcgct gccgtcctcg ttgacgggct cgccgccgaa
     2521 catgggcgag atgccgtgaa agtcgagctc gatctcgctt tccaccacgg ggttggtctt
     2581 gaaggcgttg gccggatccg ccatctgctg cggatcggcc agcacgcaaa gcttgccctg
     2641 gtagcggacg gtcaggtgct tgaagggctc caccacattg aacgccatcc cgcc
//
LOCUS       DBNHOX010000400         6091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_803094, whole genome shotgun sequence.
ACCESSION   DBNHOX010000400 DBNHOX010000000
VERSION     DBNHOX010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_803094"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="AC16UZ_08910"
     CDS             <1..849
                     /locus_tag="AC16UZ_08910"
                     /inference="COORDINATES: protein motif:HMM:NF039581.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR6246841.1"
                     /translation="EAAAEGIVKIVNENMFGALRLVSVEKGYDPRNFSLIAFGGAGPL
                     HANALGKLTQAWPVIVPPGPGVLCAYGDATTRLRDEASRTLISKLDDIEPAAIADELK
                     QLEALARQAMLAEGVGDAAAQVTDADITAQFQVDVRYSGQGLVLTVDTSEEDLLSDGF
                     AKVSEAFDRLHDQLFTFALDADKEFVNLRVVVEGPEAHVSASELEPGAADPAAASLGE
                     HQIYADGQTHAGTLYDRARLHPGNVVPGPAVIMEMDSTTLILPGHHAEVDRIGNLLIR
                     PDGHDD"
     gene            861..2744
                     /locus_tag="AC16UZ_08915"
     CDS             861..2744
                     /locus_tag="AC16UZ_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR6246842.1"
                     /translation="MTGRILQTNDAPFTRLDVDPVTLDIIENALRNARVEMDATLFRT
                     AMSPGIREQGDAFPMIANVEGKMVVGQFGSFIHGFMEAFDGVLEEGDMIFTNDPYSCG
                     AAVSHLNDWLVLKPIFKDGRHLCWAAHFGHMTDIGGKVPGSLPIDAREIFEEGVAVPP
                     VKIFRKGELQEDIISIVLRNSRMPHWNKGDFNGVIAACNMAERRCIEIAERFGDDVFY
                     SAMDELLARNCRAMTRLIQDTVPETKQVFEDYICDDGMGMGPYKIRCAMWREGDKVIF
                     DFDGTDPQSSGSINFLLNEEMFKMFCGTFMISVFDPQILFNDGFYDFMEVRIPEGCML
                     KPRRPAALSCRTHALGRIFDVISGLLGQGNPEFLCAAGFSDSPHLMYSGYDKEGEWYQ
                     LFQIGFGGIPGRPMGDGPDGHSLWPGFTNVPNEYIEAYFPLRIETYETIPDSGGPGQN
                     RGGNGIRMGYRFLEDGQISIHDDRWLTYPWGVNGGLPGGRGSKVLVRVDGTQEQLPSK
                     CDRIEVAEGDILYYNTWGGGGAGDPFEREPERVGKDVERGLVTAEGARRYGVVCDAAG
                     VVDADATEQLRSQLRAARQEIPLFDFGGTVDEIKRRCREETGLEPPDAPVFARRGSGL
                     AAG"
     gene            2751..3332
                     /locus_tag="AC16UZ_08920"
     CDS             2751..3332
                     /locus_tag="AC16UZ_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR6246843.1"
                     /translation="MPALDKTSVGLGERPALLVVDASVAFTDPACALGADFSAEIGVI
                     AQLMALAGESGWPRFFSTVWYESDDEARVFREKVPALNLLDAGSPLVAIDPRLPVDAS
                     DRVFRKTHASCFFGTDLYRWLREASVDGLVIAGFTTSGCVRASAVDALQNDYRTTVIE
                     DAVGDRDPAAHAANLYDIEAKYGDVRSLVSLLA"
     gene            complement(3337..5046)
                     /locus_tag="AC16UZ_08925"
     CDS             complement(3337..5046)
                     /locus_tag="AC16UZ_08925"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6246844.1"
                     /translation="MLDLIIRNGRIIDGTGAPAFSGDLGIVGGRIAAVGSVADDARRE
                     IDAAGRVVAPGFVDVHTHYDAQVFWDGTLSPSCYHGVTTIFGGHCGFSIAPLSTEAAP
                     YLLHMLARVEGMPVASLEAGVPWDWQSFAEYLGKLDGRLGINAGFMAGHSAIRRVVMG
                     ERAVGHQASDAEIRSMADLLDRCLAEGAMGFSSTLSPTHNDADGQPVPSRHASREELI
                     ELARVCGLHEGTSIEFLPEVGTFSEETKQLMSDLSTTAGRPVNWNVLTPGNPEVVASQ
                     LDASDVARRNGGKVVALTVPQPVLLRINLYGGMVFDAMPGWAPLFELPVPERIEALKD
                     PARRRALDASANSGGPFKELAEWRKVLVHATFRPENKQLEGRTIGSIADEQGKEPIDA
                     LFDLAISEGLRTSFMPPAFAGSGDESLWAVRGELWQDERTVIGASDAGAHLDMIDTFA
                     FSTQVLGNGVRKYGVISLEEAVHQITQVPAELFGLRERGRLESGWHADVVGFDPETVA
                     TGPTHVRSDLPAGEPRVYADAVGVDHVIVNGVEIVRDGTHTGALPGTTLRSGLDTQTP
                     RMD"
     gene            5136..>6091
                     /locus_tag="AC16UZ_08930"
     CDS             5136..>6091
                     /locus_tag="AC16UZ_08930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spinster family MFS transporter"
                     /protein_id="HIR6246845.1"
                     /translation="MQSANDDAAEWPSAARGWYATIILLIAFTFSFVDRQVLNLLVEP
                     IRDHFGISDTEVSILQGFAFAVTYVLLSVPVGRMVDRFNRVAIMIGGVLVWSATTISC
                     GLSRTYTQILVSRLGVGAGEASLAPAAWSVLSDYFRPERLSRPISVYLMGPYLGAGIA
                     MIAGAQVLDWTRDVDFVEVPLLGQLAPWQLTFIAVGLPGVLIAALVGTVREPARHGRK
                     DAELIAPPWRDVLGYLWRHRGIYTSLHLGVPCIVVMLYGLQAWTPTVLVRVYEWDLAQ
                     AGRVYGFIALFAGSAGVLSGPTVARVLERRGYPDYPLRLAVIA"
BASE COUNT          989 a         1975 c         2042 g         1085 t
ORIGIN      
        1 gaggcagcgg cggaaggcat cgtcaagatc gtcaacgaga acatgtttgg cgccctgcgg
       61 ctggtctcgg tggagaaggg ctacgatcca cgcaacttct cgctcatcgc gttcggcggc
      121 gcggggccgc tgcacgccaa cgcgctgggc aagctgaccc aggcctggcc cgtgatcgtc
      181 ccgccagggc ccggcgtgct gtgtgcctat ggcgacgcga ccaccaggct gcgcgacgaa
      241 gcttcccgga cgctgatctc caagctggac gacatcgagc cggcggccat tgccgacgag
      301 ctcaagcagc tcgaagcgct cgcccggcag gccatgctgg ccgaaggcgt cggggacgct
      361 gcggctcagg tcaccgatgc cgacatcacg gcgcagttcc aggtcgacgt tcgctacagc
      421 ggccagggcc tcgtgctcac cgtggatacc agcgaagagg atctgctctc agatgggttc
      481 gcgaaggtgt cggaggcgtt cgaccggctc cacgaccagc tcttcacctt tgcgctggat
      541 gcggacaagg agttcgtcaa tctgcgtgtg gtggtggaag ggccggaggc gcacgtttcc
      601 gcgtccgagt tggagccggg cgcggccgat cccgcggccg cctccctggg ggagcaccag
      661 atatatgccg acgggcagac ccacgcggga acgctttacg accgggcgcg actccacccg
      721 ggtaacgtgg tgccggggcc ggcggtgatc atggagatgg actcgaccac tcttatcctt
      781 ccggggcatc acgccgaggt cgaccgtata ggcaatctgc tcatccgccc ggacgggcac
      841 gacgactgag gggcgctcac atgacaggac gaatactaca gaccaacgac gctccgttca
      901 cgcggctgga cgtggacccg gtgaccctgg acatcatcga gaacgcgctt cgcaatgctc
      961 gggtggaaat ggacgccacg ctgttccgga cggccatgtc gccgggcatc cgggagcagg
     1021 gggatgcgtt ccccatgatc gccaacgtcg aagggaagat ggtggtgggc cagttcggct
     1081 cgttcatcca cgggttcatg gaagccttcg acggggtgct cgaagaaggg gacatgatat
     1141 tcaccaacga cccgtacagc tgcggcgccg cggtgagcca tctcaacgac tggctggtac
     1201 tgaagccgat cttcaaagac ggccggcacc tctgctgggc cgcccacttc ggccacatga
     1261 ccgacatcgg cggcaaagtg ccgggctctc tgcccatcga cgcccgggag atcttcgagg
     1321 aaggcgtcgc ggtgccgccg gtgaagatat tccgcaaagg cgagctgcag gaagacatca
     1381 tcagcatcgt cctgcgcaac tcgcgcatgc cccactggaa caaaggggac ttcaacgggg
     1441 tgatcgccgc ctgcaacatg gcggagcggc gctgcatcga gatcgccgag cgtttcggcg
     1501 acgacgtgtt ctactctgcc atggacgagt tgctggcccg taactgtcgg gcaatgacgc
     1561 ggctgatcca ggataccgtt ccggagacca agcaggtttt cgaggactac atctgcgacg
     1621 acggcatggg catggggccg tacaagatcc gctgtgcgat gtggcgcgaa ggggacaagg
     1681 tgatcttcga tttcgatggc accgatcccc agagctcggg ctccatcaat ttcctgctca
     1741 acgaagagat gttcaagatg ttctgcggga cgttcatgat ctcggtgttc gaccctcaga
     1801 ttctcttcaa cgacggcttc tacgacttca tggaggtgcg catccccgag ggctgcatgc
     1861 tcaagccgcg ccgacccgcg gcgctgtctt gccgaacgca cgctctcgga cgcatcttcg
     1921 acgtgatctc gggtttgctc gggcagggca atcccgagtt cctctgcgcc gccgggttct
     1981 ccgacagccc gcacctgatg tactcgggct acgacaagga gggcgagtgg taccagctct
     2041 tccagatcgg cttcggaggc attccgggcc ggcccatggg tgacgggccc gacggccact
     2101 cgctgtggcc cggcttcacg aatgttccca acgagtacat cgaggcctac ttcccgctgc
     2161 gcatcgagac ctacgagacg atccctgact ccggagggcc cggccagaac aggggcggca
     2221 acgggatccg gatgggctac cgtttcctgg aagacggcca gatctccata cacgacgatc
     2281 gctggctcac ctatccctgg ggagtcaacg gcgggctgcc cggcggccgg ggcagcaagg
     2341 tgctggtcag ggtcgacggc acccaggaac agctgccatc caagtgcgac cggatcgagg
     2401 tcgccgaagg agacatcctc tactacaaca cctggggcgg cggcggcgcg ggtgatcctt
     2461 tcgagcgtga gcccgagcgg gtgggcaagg acgtcgagcg cggcctggtc accgcggaag
     2521 gtgcccgccg ctacggcgtg gtctgcgacg ctgcgggggt cgtcgatgcg gacgccaccg
     2581 agcagctcag gtcgcagctg cgggcggcgc gccaggagat tcccctgttc gactttggcg
     2641 gcacggtgga tgagatcaag cgccgatgcc gcgaggagac cggtctggag ccgccggatg
     2701 caccggtatt tgcccgccgg ggaagcggtc tggcggcggg gtagcaggtc gtgcctgccc
     2761 tggacaagac gtcggtcggt ctgggcgagc gtccggccct tctcgtggtg gatgcgagcg
     2821 tggctttcac tgaccccgcg tgcgcgctcg gcgccgactt ctcggctgag attggcgtca
     2881 tcgcgcagct gatggcgctg gccggcgaat cgggctggcc ccggtttttc tccaccgtct
     2941 ggtacgagtc cgatgacgaa gcccgcgtgt tccgggaaaa ggtccccgct ctgaatctgc
     3001 tcgatgcggg cagccccctg gtcgccatcg acccgaggtt gcccgtggat gcctccgaca
     3061 gggtgttccg caaaacccac gcatcctgct tcttcggcac cgatctgtac cgctggcttc
     3121 gggaggcttc ggtggacggc cttgtcatag cgggattcac caccagcggt tgcgtgcgcg
     3181 cctcggcggt ggacgctctg cagaacgact atcgaacgac ggtgattgaa gacgccgtgg
     3241 gcgaccggga ccccgccgcc cacgcggcca acctctacga catcgaggcg aagtacggcg
     3301 acgttcgctc tctggtcagc ctgctggcct gaacgatcag tccattcgcg gcgtctgggt
     3361 atccagaccc gatctcaggg tggtgccggg caaggcgccg gtatgggtcc catcccggac
     3421 gatctcgaca ccgttcacga tcacgtgatc cacgcccaca gcgtcggcat agacccgggg
     3481 ctctcccgcc gggaggtccg atcgcacgtg cgttgggcca gtggccacgg tttccgggtc
     3541 gaacccgacc acgtccgcat gccagccgct ctccaggcgg ccgcgctccc gcagtccgaa
     3601 cagctctgcc ggcacctggg tgatctggtg gacggcttct tccagagaga tcacgccgta
     3661 tttgcgaacg ccgttgccga gcacctgggt cgaaaaggca aaggtgtcga tcatgtccag
     3721 atgggcgccc gcgtccgagg cgccgatgac cgttcgctca tcctgccaga gctcgccgcg
     3781 gacggcccac agggactcgt cgccgctgcc cgcgaaagcg gggggcatga acgaggtccg
     3841 caggccctcg gagatggcga ggtcgaagag ggcgtcgatg ggctctttgc cctgctcgtc
     3901 ggcaatcgag ccgatggtgc ggccctccaa ttgtttgttc tcgggtctga acgtggcgtg
     3961 cacgagcact ttgcgccact cggccagctc cttgaagggg ccgccggagt tggcgctggc
     4021 atcgagcgcg cggcgccggg cgggatcctt cagcgcttcg atgcgctcgg gaacgggcag
     4081 ctcgaacagc ggcgcccagc cgggcatcgc gtcgaacacc atgccgccgt agaggttgat
     4141 tctgagcagc accggctggg gaaccgtgag cgccaccacc ttgcctccgt tgcgtctggc
     4201 gacgtcgctg gcgtcgagct ggcttgccac gacttccgga ttgcccggtg tgagcacgtt
     4261 ccagttgacc gggcggcctg cggttgtcga aaggtccgac atcagctgct tggtttcctc
     4321 ggagaacgtc cctacctcgg ggagaaactc gatgctggtc ccctcgtgca ggccgcacac
     4381 gcgcgccagc tcgatgagct cttcgcgact ggcgtgccgg gagggaacgg gctgaccgtc
     4441 cgcgtcgttg tgggttgggg agagcgtgga ggagaagccc atggcacctt cagccagaca
     4501 gcgatccagc aggtcggcca tgctccggat ctctgcgtcg ctggcttgat ggccgaccgc
     4561 gcgctcaccc atgacgaccc ggcggatggc gctgtggccg gccatgaaac ccgcgttgat
     4621 ccccagccgc ccatcgagct tgccgagata ctcggcaaag ctctgccagt cccagggcac
     4681 tccggcctcc aggctcgcca ccggcattcc ttccacgcgg gccagcatgt ggagcaggta
     4741 gggggccgcc tcggtgctca gcggcgcgat gctgaaaccg caatggccgc cgaatatggt
     4801 ggtcacgccg tggtaacacg acgggctcag cgtgccgtcc cagaagacct gggcatcgta
     4861 atgagtgtgc acgtccacga agccgggggc gaccacccga cccgccgcgt ctatttcccg
     4921 ccgcgcgtcg tcggcgaccg agccaaccgc ggcgatgcgt ccgccgacta tgccgagatc
     4981 gccgctgaac gccggcgcgc cggtgccgtc gatgatgcgg ccgttcctga tgatcagatc
     5041 gagcatctcg ctcctcccgt tgttagcccg taagatatag ctttccggcg cgttaccaaa
     5101 gcccggtgat cagctcaagc ccgggagggg cccctttgca gtctgccaat gacgacgcgg
     5161 ccgaatggcc gtccgccgcg cggggatggt acgccaccat catcctgctc atcgccttca
     5221 ctttctcttt cgtggatcgg caggtactga acctcctggt cgagcccatt cgggatcact
     5281 tcgggatctc cgatacggaa gtcagcatcc tccagggctt tgcgttcgca gtcacctacg
     5341 tgcttctgag cgttcccgtg gggcgcatgg tggatcggtt caaccgcgtg gccatcatga
     5401 tcggcggcgt gctggtctgg agcgcgacca ccatctcctg cgggctgtcg cgcacctaca
     5461 cccagatact cgtctcgcgc ctgggcgtgg gggctggcga ggcttcgctg gctcccgccg
     5521 cttggtccgt gctgtcggac tactttcgcc cggagcgtct ttcgcgcccc atcagcgtct
     5581 acctgatggg tccgtacctc ggcgccggta ttgccatgat cgcaggcgca caggtgctcg
     5641 actggacccg cgacgtcgac ttcgtggagg tgcctttgct gggccagctg gcgccatggc
     5701 agctgacctt catcgcggta gggctgcccg gcgtgctcat cgcggcgctg gtcggcacgg
     5761 tgcgtgaacc agcccgtcat ggtcgcaagg acgccgagct catcgccccg ccctggcggg
     5821 acgtgcttgg ctacctgtgg cgacacagag gcatctacac ctccctgcat ctcggggtgc
     5881 cctgcatcgt ggtgatgctt tacgggctgc aggcgtggac gcccacggtg cttgtgaggg
     5941 tctacgagtg ggatctggct caggcggggc gggtctacgg cttcatcgcg ctctttgccg
     6001 gttccgcggg ggtgctgtcg gggcccaccg tggcacgtgt tctggaacgg cgcggatacc
     6061 ccgactaccc gctgcggctt gccgtgatcg c
//
LOCUS       DBNHOX010000401         3465 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_417455, whole genome shotgun sequence.
ACCESSION   DBNHOX010000401 DBNHOX010000000
VERSION     DBNHOX010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_417455"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="AC16UZ_08935"
     CDS             complement(<1..608)
                     /locus_tag="AC16UZ_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246846.1"
                     /translation="MKDTLSVRFLAEFVHRRGDLHARLDGRARAEEGIQAQRKLQRGR
                     GPAYERERRVALTREVGGRAHLISGRVDGADLTARLVEEYKTTRADPELAQQKLGSAH
                     WAQVKLYGGMLVEELDGASDSQWTLRLVYCHPDTLATREFDQQLSGHELATFLDETLD
                     SYAGWVAMQLAHERNRNEAIARRDFPFPRYRPHQQAVARRVYR"
     gene            complement(605..2221)
                     /locus_tag="AC16UZ_08940"
     CDS             complement(605..2221)
                     /locus_tag="AC16UZ_08940"
                     /inference="COORDINATES: protein motif:HMM:NF020354.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HIR6246847.1"
                     /translation="MASSIVPQLAANSPPPPDYYAENLRQLITHVHAHYGDVLRREEI
                     GYGRRIVACSVPAQRLYARLLSRRGPWIRIDKLRYREVSDVTAAVGELEEAGLVVRNG
                     RAPADALLGLLSTAERRELFAAIGDAPKARWINQCVARYSDAHVLSRLAGVHPWVSIR
                     DFPTFWLHQLLFFGDTRQDLSAFVLQDLGLVRYEPYELDPRHRQFANRERLDEYLRYL
                     ELSSLSYRLEEQPVLASWLSGAMWTEPASRWAQRSRDKTANRLGRWHERRGEYDQALA
                     SYARSTSHPARERTARILDRLGDAEGVQDVLHAMLSEPRSAEEEDFARRFGPNGRRKR
                     HAHRLERLPDGDGVRVEQLAVDLLTEGGGRGWHLENRFSLGLAGLAFWPVVFADVPGA
                     FVNPFQQGPVDLFWPDFVEQRAGILEVQLDKLATQGGLASVVMDTWRRKFGVANRLVS
                     WAHFDEDLLEAVLGQLPQGDLLRLADFTLRNLHRLRKGFPDLLVIRAPGDYEFVEVKG
                     PTDQLQPVQRIWLDALEELDLPARVLRFKA"
     gene            2294..2740
                     /locus_tag="AC16UZ_08945"
     CDS             2294..2740
                     /locus_tag="AC16UZ_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246848.1"
                     /translation="MPAFSYCICTTVDARALQALLEASGQGSYKDEQPWLIAREILEE
                     AASQSARLPILFASGHPSEFSHWGFIESIDVVELHRGTWESRCAFSPLQPVNPIWTSI
                     DSVFLKPTGEQLRREALEGIAHHRYPLNEGELHPYAICETPAFILA"
     gene            complement(2771..2995)
                     /gene="csrA"
                     /locus_tag="AC16UZ_08950"
     CDS             complement(2771..2995)
                     /gene="csrA"
                     /locus_tag="AC16UZ_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HIR6246849.1"
                     /translation="MLVLTRRAGESIMIDDDIELKVLKIRGSQVHLGIDAPKRSAVHR
                     KEVWLRINGEPKAAGREEKEDSSDPSRGVA"
     gene            complement(3109..>3465)
                     /gene="rmuC"
                     /locus_tag="AC16UZ_08955"
     CDS             complement(3109..>3465)
                     /gene="rmuC"
                     /locus_tag="AC16UZ_08955"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR6246850.1"
                     /translation="FIDAFDKRIVIVSPTTLMMTLRIIHNVWRFEKQNRNAQDIARRA
                     GALYDKLRGLIEDMDLLGKQLGTVEKTYQAAYGKLASGKGNLVRQVERFRELGAQVKR
                     PLPKSLLDEATDRSDD"
BASE COUNT          627 a         1133 c         1076 g          629 t
ORIGIN      
        1 cgatacaccc gccgggccac cgcctgctga tggggacgat agcgtgggaa cgggaagtcg
       61 cgacgcgcga tcgcctcgtt ccggttccgc tcgtgggcga gctgcatcgc cacccatccg
      121 gcgtagctgt cgagggtctc atcgaggaac gtggcgagct catgcccgga caattgctga
      181 tcgaactcgc gggtggcgag cgtgtcggga tggcagtaca ccagccgcaa cgtccattgg
      241 ctgtccgatg cgccatcgag ttcctccacc agcatcccgc cgtaaagctt gacctgcgcc
      301 caatgggcgc ttccgagttt ttgctgggca agttccgggt cggcccgggt ggtcttgtac
      361 tcctcgacga gcctggcggt gagatcggcg ccgtccacgc gcccggatat caggtgcgct
      421 cgacccccga cctcccgcgt gagcgcaacg cgacgttcgc gctcgtatgc cggcccgcgt
      481 ccgcgctgca gcttccgctg agcctgaatc ccctcctccg cccgggcccg cccatccagg
      541 cgagcgtgca gatcaccgcg ccgatgcacg aactccgcga gaaaacgcac tgaaagcgtg
      601 tctttcatgc cttgaaccgc agcacgcgag cgggcaggtc aagttcttcc agagcatcga
      661 gccagatccg ctgtaccggc tgcagctgat cggtgggccc tttgacctcc acgaactcgt
      721 aatcccccgg ggcgcggatc acgagcaggt ccgggaaccc cttacgcaga cgatgcaggt
      781 tgcgcagggt gaaatccgcc aggcgcagca gatcgccctg aggcagctga ccgagcaccg
      841 cttccagcag atcctcgtcg aagtgcgccc agctcaccag ccggttggca acgccgaact
      901 tgcggcgcca ggtgtccatg acgacgctgg ccagcccgcc ctgagtggct agcttgtcca
      961 gctgaacctc gaggatgccg gcgcgctgct ccacgaaatc cggccagaag aggtccaccg
     1021 ggccttgctg gaacgggttg acgaaggcgc cgggaacgtc ggcgaacacc accggccaga
     1081 acgccaggcc ggccaggccc agagagaatc gattttccag atgccagccc cgcccaccgc
     1141 cctcggtcag caggtccacg gccagctgct cgacccggac accatccccg tcgggtaggc
     1201 gctcgagccg atgagcgtgg cgcttgcgcc ggccgttcgg cccgaaccgt cgggcaaagt
     1261 cctcttcctc cgccgagcgc ggctcgctga gcatggcatg gagaacgtcc tgcacgccct
     1321 cggcatcgcc gagacggtcg agtatacgtg ccgttcgctc ccgagccgga tgactggtgc
     1381 tgcgcgcata gctggcgagc gcctggtcgt actcgccccg tcgttcgtgc cagcgaccca
     1441 gccggttggc cgtcttgtcc cgcgatcgct gagcccatcg cgacgccggc tcggtccaca
     1501 tcgccccgga gagccatgat gcgagcacag gctgctcttc caggcgatag gaaaggctgc
     1561 tgagctcgag ataccgcagg tattcgtcga gccgttcacg gttggcgaac tgccgatgcc
     1621 ggggatcgag ctcgtagggc tcgtaccgga cgaggcccag gtcctggaga acgaaggcgc
     1681 tgagatcctg gcgggtatcc ccgaagaaca gcagctgatg cagccagaag gtgggaaagt
     1741 cgcgaatgct cacccatgga tgcacaccgg cgagccgaga gagaacgtga gcatcgctgt
     1801 aacgcgctac gcattggttg atccagcgag ccttcggcgc atcgccaatg gcggcaaaga
     1861 gctcacgtct ttccgccgta gagagcagac cgagcagcgc gtcggccggt gcccggccgt
     1921 tgcggacgac gagcccggct tcctccagct cgccaaccgc ggccgtcaca tcgctcactt
     1981 cgcgatagcg aagcttgtcg atacgaatcc acggcccacg tcggctcaga aggcgggcat
     2041 acagccgctg ggcgggaacc gagcaggcca cgatgcggcg cccgtagccg atctcctctc
     2101 tgcgcagcac gtcaccatag tgggcgtgca cgtgggtgat cagctggcgc aggttctcag
     2161 cgtagtagtc gggcggcggc gggctgttgg cggcaagctg aggcacgatg cttgaagcca
     2221 tccggttgct tcgaacgagg cctgtggatt ataaagaggt ctgtatacat gtacagtact
     2281 cgccagctcc ccaatgcctg ccttttccta ctgcatctgc accaccgtcg acgcccgcgc
     2341 cctgcaggcg ctgctggaag caagcgggca aggcagctac aaggacgagc agccctggct
     2401 catcgctcga gagattctgg aagaagccgc gtctcagtca gcccgcctgc ccatcctttt
     2461 cgccagcggg catccttccg agttctcgca ctggggattc atcgaatcca tcgacgtggt
     2521 cgagctgcac cgcggaacct gggaatcacg atgcgcgttc agcccgctgc aacccgtaaa
     2581 ccccatctgg acgagcatag acagcgtctt cctgaagccc acgggcgagc agctcagacg
     2641 agaggcgctg gaaggaattg cgcaccatcg atatccgctc aacgaaggcg agctgcatcc
     2701 gtacgcgatc tgcgagacgc cagcgttcat actggcttaa aggagagtga tcgggttgag
     2761 ctgaggccgc tcaggcgacg ccgcggctgg gatctgacga gtcttctttt tcttcgcggc
     2821 ctgccgcttt cggctcgccg ttgatgcgca gccatacttc ctttcggtgc acggcagaac
     2881 gtttcggcgc gtcgattccc agatgcacct ggctaccgcg aatcttgagc actttgagct
     2941 ctatatcgtc gtcgatcatg atcgactccc cagcacgacg ggtgaggacc agcatccagg
     3001 tttccttgca atccttttct ccgtagaaat ctactgacat tgactgatcc gttctgtgac
     3061 gtgcacgcgg ctttggccgg gcagcggcga gaaaggagaa ctggttggct aatcgtctga
     3121 gcggtcggtc gcttcatcca gaagggactt gggcagcggc ctcttgacct gggcgcccag
     3181 ctcacggaag cgctccacct gcctgacgag gttgcccttg ccgctggcga gctttccgta
     3241 ggctgcctgg taggttttct cgacggtgcc aagctgcttg ccgagcagat ccatgtcctc
     3301 gatcagtcct ctgagcttgt cgtaaagagc ccccgcccgc cgcgcgatgt cctgggcatt
     3361 ccggttctgc ttttcgaacc gccagacgtt gtgaatgata cgcagcgtca tcatcaaggt
     3421 agtggggctt acaatcacga tgcgtttgtc gaacgcgtct atgaa
//
LOCUS       DBNHOX010000402         4121 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_739291, whole genome shotgun sequence.
ACCESSION   DBNHOX010000402 DBNHOX010000000
VERSION     DBNHOX010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_739291"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="AC16UZ_08960"
     CDS             complement(<1..232)
                     /locus_tag="AC16UZ_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246851.1"
                     /translation="MKAPRRPTAQAAIRAAAVAGLLLAGCDTGSGAEPASPELLMARP
                     PEGWKVWSATQDPGLRVAQYVPADTDADNWEQM"
     gene            complement(232..1167)
                     /locus_tag="AC16UZ_08965"
     CDS             complement(232..1167)
                     /locus_tag="AC16UZ_08965"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003157311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6246852.1"
                     /translation="MQSRPACFVAALLLAFVPAANAADWYPSRYGADDTLGAINNLSP
                     EKVVQAARLVTEGKTYPLGVETGPDSPAYPPRSYSLTVLQPGDGTGSLMGTNRASGND
                     DLLSFWMGIGSQIDGLGHMGIEHVYYNGNRAQDFVSATGLSRLSIDRLPPIVGRGVLI
                     DMAAHMGKAILPAGTAFNSREIKAAAKAQGVTIGTGDVVLFHTGWLNVADDKARFMAG
                     EPGLGVDGARYLAGLGVVAVGSDTWGLEVLPSEVEGEAFPVHPELLAKNGVYILENMD
                     TRALAADGVHEFLFVLGQPRFVGAVQAVINPVAIK"
     gene            complement(1212..2615)
                     /gene="mpl"
                     /locus_tag="AC16UZ_08970"
     CDS             complement(1212..2615)
                     /gene="mpl"
                     /locus_tag="AC16UZ_08970"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HIR6246853.1"
                     /translation="MNAERRIYFLGIGGTLMGSLAQLAREMGYRVSGSDVAIYPPMSD
                     QLAAADIEVFEGFDPAHLDPEPELVVIGNAGLPRGHPGVEHIIENGLRYVSGAEWLGS
                     QVMPGRWVLAVTGTHGKTTTASMLAWILDQAGLKPGYLIGGVPKNFPRSSRLGDDPFF
                     VVEADEYDCSYFDRRSKFVHYHPRTLIINNLEYDHADIFPNLAAIQTQFNHLLRTVPG
                     HGLVIAPSNDDNVNETLQQGCWTPIARVGAPGVKQPLERDNGERWSASRGDGTGAEFD
                     VCLGEENLGTVRWPIFGDFNVRNALAAIAAARHAGVPPEVAIDALGSFEGVKRRLELI
                     AEIGATLIYDDFAHHPTAIRATLQGLRNKVGSEEIIAVIEPRSHTMSLGTLRNELATC
                     CSPADEVYWFRGENIQWDLSELLKSCVIPAHINFELDKLIEALAQLPQKRRHIVIMSN
                     GSFGGIYQKLPTRLRSG"
     gene            complement(2612..3112)
                     /locus_tag="AC16UZ_08975"
     CDS             complement(2612..3112)
                     /locus_tag="AC16UZ_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246854.1"
                     /translation="MKAVLQLFWQICRLKQSPEYVPTQTWFVLLVIAANLICSAAVSL
                     SAEGAPGLLTTINSIVVGQTTTAALVWVALNLRDMGGRFLATITALFGCDLIITAVFA
                     LILPLTLLAPTMAPVLFLLFVAWSVSVAGFILHRALQVPLALGIVVALGMSVLSLALS
                     QLAIGA"
     gene            3185..>4121
                     /locus_tag="AC16UZ_08980"
     CDS             3185..>4121
                     /locus_tag="AC16UZ_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018404836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR6246855.1"
                     /translation="MKRANAFYAQSGGVTAVINASACGVIQTARKHRDRIGKVLAGRN
                     GIIGALAEELIDTGRESAKDIAGLRTTPSGAFGSCRYKLKSLEENEREYRRLIDVFRA
                     HDIGYFFYNGGGDSQDTAHKVSQLGGRLGYPITCIGIPKTVDNDLPFTDCCPGFGSVA
                     KYVAVSIREAAMDVASMCETSTKVFILEVMGRHAGWIAAAGALAQEGPRDAPHIILLP
                     EVPFDRKRFLAKVRETVDGCGYCVIVVSEGAQYPDGTFLADAGSKDAFGHTQLGGVAP
                     TLAAMIKDELGYKYHWAVADYLQRAARHVASATDVE"
BASE COUNT          706 a         1301 c         1410 g          704 t
ORIGIN      
        1 gcatctgctc ccagttgtcg gcgtcggtgt cggccgggac gtattgggcc actcgcagcc
       61 ccggatcctg ggtggcagac cataccttcc agccctccgg tggtcgggcc atgagcagct
      121 ccggggaagc cggctcggcg ccggagccgg tgtcgcatcc ggcaagcagc aggccggcca
      181 cggcggccgc ccggatcgcg gcctgggcgg tcgggcgcct cggggccttc atcacttgat
      241 cgccaccgga ttgatcaccg cctggacggc gcccacgaag cgaggctgtc cgagcacgaa
      301 gaggaactcg tgcacgccgt ccgcggccag ggcccgggtg tccatgttct ccaggatgta
      361 gacgccgttc ttggcgagca gctcgggatg aaccggaaag gcttcgcctt cgacctcgga
      421 aggcagcacc tcgagccccc aggtgtccga ccccaccgcc acgacgccga gaccggccag
      481 atagcgtgcc ccgtctacgc ccagaccggg ctcgccggcc atgaagcgcg ctttgtcatc
      541 ggcgacgttc agccagccgg tgtggaaaag caccacgtct cccgtgccga tggttacgcc
      601 ctgggccttg gctgcggcct tgatctctcg gctgttgaag gcggtgccgg cggggagaat
      661 ggccttgccc atgtgggcgg ccatgtcgat cagcacgccg cggccgacga tgggcggcag
      721 gcggtcgatg ctcagcctcg acaggccggt tgcgctcacg aaatcctggg cccggttgcc
      781 gttgtagtac acgtgctcga tgcccatgtg gccgagcccg tcgatctggc tgccgatgcc
      841 catccagaaa ctcagcagat cgtcgttgcc ggacgccctg ttggtaccca tcagggatcc
      901 ggtgccgtcg ccgggctgca gcacggtgag cgaatagctt cgcggcgggt aggcgggcga
      961 gtcggggccg gtctccaccc ccagcgggta ggtcttgcct tccgtgacca gacgggccgc
     1021 ctgaacgacc ttctcaggag acaggttgtt gatggccccc agggtgtcgt cggcgccgta
     1081 gcgcgacggg taccaatccg ctgcgttggc agcagggacg aatgcgagca gcagcgctgc
     1141 gacgaagcat gccggtctgg attgcatggc ggggcctcct ggcgaaagtg ggctggaagt
     1201 ggtgcccgag tttagccgct gcgcagacgg gttgggagct tttggtagat cccgccgaag
     1261 ctgccgttgc tcatgatcac gatgtggcgg cgcttctggg gcagctgggc cagggcctcg
     1321 atgagcttgt cgagctcgaa attgatgtgc gcagggatca cgcagctctt cagcagctcc
     1381 gagaggtccc actgaatgtt ctccccgcga aaccagtaga cctcgtccgc cggcgagcag
     1441 cacgtggcga gctcgttacg cagggtcccc agtgacatgg tgtgggagcg gggctcgatg
     1501 acggcgatga tctcctcgct gcctaccttg ttgcgcaagc cctgcagcgt tgcgcggatg
     1561 gcggtggggt ggtgagcaaa gtcgtcgtag atcaacgttg cgccgatctc ggcaatgagc
     1621 tccaggcgcc tcttgacgcc ttcgaagctg cccagggcgt ctatggcgac ctccggcggc
     1681 acgccggcgt gccgggcggc agctatggcg gccagggcgt tgcggacgtt gaagtcgccg
     1741 aagatgggcc agcgcaccgt gcccagattt tcctcgccca ggcagacgtc gaactcagca
     1801 cctgtgccgt ctcccctgct ggcgctccac cgctcaccgt tgtcgcgttc cagcggttgc
     1861 ttcaccccgg gggcgcccac gcgggcgatg ggcgtccagc aaccctgctg gagggtctcg
     1921 ttgacgttgt cgtcgttgga cggcgcgatg acgagcccgt ggccgggcac ggtgcgcagc
     1981 aggtgattga actgggtctg gatggcggcg aggttgggga agatgtccgc gtggtcgtac
     2041 tccagattgt tgatgatcag ggtgcgcgga tggtagtgaa cgaacttgga tctgcggtcg
     2101 aagtacgagc agtcgtactc gtcggcctcc accacgaaaa aggggtcgtc gcccagccgg
     2161 gacgagcgcg gaaagttctt gggcacgccg ccgatcaggt agccgggctt cagccccgcc
     2221 tggtcgagaa tccaggccag catgctggcc gtcgtggtct tgccgtgggt gcccgtgacg
     2281 gccagcaccc agcggccggg catcacttgg ctgccgagcc attcggcacc ggacacgtag
     2341 cgcagcccgt tctcgatgat gtgctcgacg cccggatgac ccctgggcag gcccgcgttg
     2401 ccgatcacca ccagctccgg ctcgggatcc aggtgggcgg gatcgaagcc ttcgaacacc
     2461 tcgatgtcgg cggccgccag ctgatcgctc atgggcgggt agatggccac gtccgacccg
     2521 gagacccggt atcccatctc cctggccagc tgggccaggc tccccatcag ggtgccgccg
     2581 atgcccagaa aatagattcg gcgttctgcg ttcatgcgcc gatggccagc tggctcaatg
     2641 ccaggctgag aaccgacatg cccagggcca cgacgatccc gagcgcaagc gggacctgca
     2701 acgcgcgatg cagaatgaag ccggcaacgc tcacgctcca ggcgacgaag agcaggaaca
     2761 gcacaggcgc catggtcggt gcgagcaggg tgagcggcag gatgagggcg aagactgccg
     2821 tgatgatgag gtcgcagccg aacagcgccg tgatggttgc aaggaaccgc ccacccatgt
     2881 cgcgcaggtt caacgccacc cacaccaggg ccgccgtcgt cgtctggccc acgacgatgc
     2941 tgttgatggt ggtcagcagg ccgggcgcgc cttcggcgga cagagaaacg gcagcgctgc
     3001 agatcaggtt tgcggcgatg accagcagca cgaaccacgt ctgggtggga acgtactcgg
     3061 gactctgctt cagccggcat atctgccaga aaagctgcaa gacggctttc atcggcgaaa
     3121 ttgtcgcaca aaactttctc ttcacggaca atgaccgctc gttttccgga cgagacgtcg
     3181 agggatgaag agagccaatg cgttctacgc tcagtcgggc ggggtcacgg ccgtcatcaa
     3241 cgcatccgca tgcggggtca tccagaccgc gcgcaagcat cgcgatcgca tcggtaaggt
     3301 cctcgccgga cgcaacggga tcatcggcgc gctcgccgag gagctgatcg acacgggccg
     3361 ggagagcgcc aaggacattg ccgggctgcg cacgacgccg agcggggctt tcggatcctg
     3421 ccggtacaag ttgaagtcct tagaggagaa cgagcgcgag taccggcgcc tgatcgacgt
     3481 ctttcgagcc cacgacattg gttacttttt ctacaacggc ggcggggatt cccaggacac
     3541 tgcccacaag gtgtcccagc tgggcgggcg gctcggatat ccgatcacct gcatcgggat
     3601 tcccaagacg gtcgacaacg atcttccgtt cacggactgc tgtcccggct tcggatccgt
     3661 tgccaagtac gtcgcggtat ccatccgcga agcggccatg gacgtcgcgt cgatgtgcga
     3721 gacctccacc aaggtcttca ttctggaggt catggggcgt cacgcgggtt ggatcgcggc
     3781 ggcgggagcc ctggcccagg aagggccgcg cgatgcgcct cacatcatcc tgctgccgga
     3841 ggtgcccttc gatcggaaac gttttctcgc caaggttcgg gagaccgtgg acgggtgcgg
     3901 ttactgcgta atcgtcgtct ccgaaggagc tcagtacccc gacggcacct tcctggccga
     3961 tgccggaagc aaggatgcct tcggccacac ccagctgggt ggagtggcgc cgacgctggc
     4021 ggccatgatc aaagacgagc tcggctacaa ataccattgg gcggtggccg actacctgca
     4081 gcgggcggcc aggcacgtgg cctcggccac cgatgtggag c
//
LOCUS       DBNHOX010000403         2023 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_161434, whole genome shotgun sequence.
ACCESSION   DBNHOX010000403 DBNHOX010000000
VERSION     DBNHOX010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_161434"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1451)
                     /gene="aceA"
                     /locus_tag="AC16UZ_08985"
     CDS             complement(147..1451)
                     /gene="aceA"
                     /locus_tag="AC16UZ_08985"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019361792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HIR6246856.1"
                     /translation="MALHFIPRKPREEQVRELQTEWDTNPRWKDVKRGYTAADVVNLR
                     GTIPHRSMLAAHGADKLWDYLQNEDFINSLGAVTGGQAVQQVKAGIKAIYCSGWQVAA
                     DANDSETMYPDQSLYAVNSVPTLVNRINNAFRRADEIQWSKGIDDGIDYFAPIIADAE
                     AGFGGILNAFELMKSMIGAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLIAAR
                     LAADVCDVPTILLARTDANAADMVTSDIDPWDKDFLTGERTPEGFYKTHAGLDQAISR
                     GLAYAPYADLLWCETAVPDLDEAKRFAEAIKKEYPDQMLSYNCSPSFNWRANLDDATI
                     AKFQKELAAMGYKYQFITLAGIHSMWFNMFNLAQGYASRQMSAYVELQEEEFAAADRG
                     YTFVSHQQEVGTGYFDQVATVIQGGQSSVTALTGSTEEAQFH"
     gene            complement(1591..>2023)
                     /locus_tag="AC16UZ_08990"
     CDS             complement(1591..>2023)
                     /locus_tag="AC16UZ_08990"
                     /inference="COORDINATES: protein motif:HMM:NF051921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF7931 domain-containing protein"
                     /protein_id="HIR6246857.1"
                     /translation="LAACVAEPTRALLIYSQYLDHSLFDRPEIVDAVSRFVRSAPPAT
                     LRILIHSSQPMVSRGHKLLELARRIDSKIEIRKVPDGLATDVHTYLVWDQRGYWLMPD
                     YREYDGLTNLYDPVQASKLTERFDYLWNLSSPDPELRILRI"
BASE COUNT          345 a          604 c          660 g          414 t
ORIGIN      
        1 tgctggcgga gtatccgcag tacccgtgcg cggggtctta cggggagctg caggcgcttt
       61 taaaggctgc ggcctgacgg cccgatcgct tcaacgggtg gcgcgctgcg ccgccgggca
      121 ttgcgcccgg cagcgacggc cgtgtgtcag tggaactgag cttcttccgt tgaaccggtg
      181 agagcggtca ccgagctctg cccgccctga atgacggtgg cgacctggtc gaagtagccg
      241 gtgccgactt cctgctggtg gctgacgaac gtgtaccccc tgtcggcggc ggcaaactct
      301 tcctcctgga gctccacgta ggcggacatc tgcctgctcg catacccctg cgccaggttg
      361 aacatgttga accacatgct gtgaatgcct gccagcgtga tgaactgata cttgtaaccc
      421 atggccgcca gctctttctg gaacttggcg atggtcgcgt cgtccaggtt ggccctccag
      481 ttgaaggacg gcgagcagtt gtacgacaac atctggtcgg ggtattcctt ctttatcgcc
      541 tcggcgaacc gcttggcctc gtccagatcc ggtaccgcgg tttcgcacca cagcagatcg
      601 gcgtacggcg cgtaggccag gccgcgagag atggcctgat caaggcccgc gtgggtcttg
      661 tagaaccctt ccggcgtgcg ctctcctgtc agaaaatcct tgtcccaggg gtcgatgtcg
      721 ctggttacca tgtcggcggc gtttgcgtcc gtgcgcgcga gcagtatggt cggcacgtcg
      781 cacacgtcgg cggccagacg tgcggcgatc agcttctgca ccgcctcctg ggtgggtacc
      841 agcaccttgc cgcccatgtg gccgcacttc ttcaccgagg ccagctggtc ctcgaagtgc
      901 acgccggcag ccccggcgcc aatcatggat ttcatgagct cgaaggcgtt gaggatgccg
      961 ccgaagcccg cctcggcatc ggcgatgatc ggtgcgaagt agtcgatgcc gtcgtcgatt
     1021 cctttcgacc attgaatctc gtcggcccgc cggaacgcgt tgttgatccg gttgaccagg
     1081 gtaggaacgg agttgaccgc gtagagggat tggtccgggt acatggtctc cgagtcgttg
     1141 gcgtccgcgg ccacctgcca gcctgaacag tagatggcct tgatgcccgc cttgacctgc
     1201 tgaacggcct ggccgccggt gacggcgccg agagagttga tgaagtcctc gttctgcagg
     1261 tagtcccaga gcttgtccgc gccgtgagcg gccagcatgc tgcggtgggg tatggtgccc
     1321 ctcagattga cgacgtcggc cgccgtgtaa ccgcgcttga cgtctttcca gcgcggattg
     1381 gtgtcccact ccgtctgcaa ttctctgacc tgctcttcgc ggggcttccg cggaatgaaa
     1441 tgtagcgcca tggatcgttc gcccgtctca aggaatgtcg gggccgggat tgtagagacg
     1501 cagattgaaa agaatagcga aaggaaaaca attaatgctt tcccaatggg aatatataag
     1561 cgacgtgaac gacggcgccg gttgcccgtc tcagatacgc aggatacgca gctccggatc
     1621 ggggctgctg aggttccaca ggtagtcgaa ccgctcggtg agctttgatg cctggacggg
     1681 atcgtagagg ttggtcagac cgtcatactc ccggtagtcg ggcatcagcc agtagccgcg
     1741 ctggtcccac accagatagg tgtgaacgtc cgtggcgagc ccgtctggca ccttacggat
     1801 ttcaatcttg ctgtcgatcc gtcgggcgag ctccaggagc ttgtggccgc ggctcaccat
     1861 gggctggctg gaatggatga ggattctcaa cgtggccggc ggcgcgctgc gaacgaacct
     1921 cgagaccgca tcgacgatct cgggccggtc gaacagcgag tggtccagat actggctgta
     1981 gatcagcagc gccctggtcg gctcggccac gcacgcggcg agg
//
LOCUS       DBNHOX010000404         3020 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1061352, whole genome shotgun sequence.
ACCESSION   DBNHOX010000404 DBNHOX010000000
VERSION     DBNHOX010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1061352"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="AC16UZ_08995"
     CDS             <1..236
                     /locus_tag="AC16UZ_08995"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6246858.1"
                     /translation="LKVFALIAELSPQAALRTATSSAARALGVHGMTGRLVPGLAADI
                     LVVDGNPLDDLDALANPVGVWARGRRVVDPHGA"
     gene            complement(277..1116)
                     /locus_tag="AC16UZ_09000"
     CDS             complement(277..1116)
                     /locus_tag="AC16UZ_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6246859.1"
                     /translation="MELKATEYSVASGIATVRLNRPHRLNAWTGRMHSEYRWCIDRAE
                     RDPAVRAIVVTGNGRGFCVGGDAEALEGHARKGGYDPGTPARLAAPGYGRAPEFDASF
                     AYHYGLSKPVIAAVNGPAAGVGLALACFADLRFAAAGVKLTTAHGKLNLPAEYGLSWL
                     LPRMIGLTRANDLLLSSRVFLAEEALAMGLVNAVYPQAELMDEVYAYTRELIRTVSPA
                     SLAQTRWQIYRDLHRSVAESVRASEQLLDEMMRQEDYREGVAAFLEKRAPSWSGSAGE
                     TGE"
     gene            complement(1150..2235)
                     /locus_tag="AC16UZ_09005"
     CDS             complement(1150..2235)
                     /locus_tag="AC16UZ_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6246860.1"
                     /translation="MMVMRILALVLMFGLAGCGAPQQTAPEAVPDPDKVYRWKLITTW
                     PKNLPALGMAPERLAENLRVMSRGRLDIRVYGAGELVGAFEVFDTVRQGTAEMGHGAA
                     YYWRGKLPVAAMFATVPFGMMAQEMNGWLRYGGGMELWREAYEPFGLVPLAAGNSGVQ
                     MAGWFNKEINSLEDLQGLKMRIPGLGGEVLKRAGGVPVSMAGSEIYTAMQTGVIDATE
                     WVGPYNDLAMGLQDVARYYYYPGWHEPGPTLEAIVNKAAWDSLPEDLQRMVEVAAAAA
                     NEDLLSEFTARNASALKTLLSKHNVELRRLPDDVLAKLKVESEAVVVEATGDSDLARR
                     IRESYGRFLSDVREYHAISEQAYINAR"
     gene            complement(2253..>3020)
                     /locus_tag="AC16UZ_09010"
     CDS             complement(2253..>3020)
                     /locus_tag="AC16UZ_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR6246861.1"
                     /translation="ESLELAELCERMLTRTGLLEFHGREAGERGAARVDNLEELVSAC
                     RQFSGEQVFPLLADAAEQTSILEEFLDQVALDAGDRQAEAGPAVQMMTLHSAKGLEFP
                     MVFLAGMEEGLFPHRMSAEEPGRMEEERRLCYVGITRAKQTLYLTHAETRRLHGTDTY
                     NRPSRFLLEIPTELVHEVRMGGAVQRPYGRPAEAGHAVGAEGGLRMGQRVLHAKFGEG
                     VVLQAEGSGERARVQVNFAGAGAKWLMLGYANLQALD"
BASE COUNT          486 a         1059 c          962 g          513 t
ORIGIN      
        1 cattgaaggt cttcgccctg attgccgagc tcagccccca ggccgctttg cgaaccgcga
       61 cgtcatccgc agctcgagcc ctgggcgttc acgggatgac ggggcgcctc gtgccgggtc
      121 ttgccgccga cattctggtg gtggatggaa atcctctcga cgacctcgac gccctcgcaa
      181 acccggtggg ggtctgggcc cgcggccgga gggttgtcga cccgcatggc gcctgagcaa
      241 cggggcgcct gggtcgaacg gccccgagcc aaacgattac tcgccagtct ctccagctga
      301 tccggaccag ctgggggcgc gtttctccag aaacgccgcc accccttcgc gatagtcttc
      361 ctgccgcatc atctcatcga gcagctgctc agaggctctg acggactcgg caacgcttcg
      421 atgcaggtcc cggtatatct gccagcgggt ctgggccagg gacgccgggg acaccgtgcg
      481 gatgagctcc ctggtgtaag cgtagacctc gtccatgagc tccgcctggg ggtacacggc
      541 gttgacgagc cccatggcga gggcttcctc ggcgaggaag acacggctcg acagcagcag
      601 gtcgttcgcc cgggtgaggc cgatcatccg tggcaacagc caggacagcc cgtactccgc
      661 ggggaggttc agcttgccgt gggcggtggt gagcttgacg cccgcagcgg cgaaacgcag
      721 gtctgcgaag caggcgagcg ccagaccgac ccccgcggcc gggccgttca cggctgcgat
      781 caccggtttg gaaagcccgt agtgataggc aaagctggcg tcgaattccg gcgcccgacc
      841 gtatcccggc gctgcgagcc gggcaggcgt tcccggatcg tagccgccct tgcgcgcgtg
      901 cccttccagc gcttcggcgt ctcccccgac gcagaaaccc ctcccgttgc ccgtgacgac
      961 aatcgcgcgg accgctgggt cacgctccgc gcgatcgatg caccagcggt actcgctgtg
     1021 cattcgaccc gtccacgcgt tgaggcgatg aggccggttc aggcgcacgg tggcgatgcc
     1081 ggacgcgacc gagtactccg tcgccttcag ctccaacgcg acctccccgc cggccggtcg
     1141 cgcagaggct cagcgggcat tgatgtaggc ctgctcgctg atggcgtggt actcgcgcac
     1201 gtcgctgaga aaccgcccgt aggattcacg aatacgacgg gcaagatcgg agtcaccggt
     1261 agcctcgacc accaccgcct cggactcgac tttaagcttt gccagcacgt cgtccggcaa
     1321 gcgcctgagc tccacgttgt gcttcgaaag aagcgttttg agggcgctgg cattgcgggc
     1381 ggtgaactcg ctcaggagat cttcgttggc ggcagccgcg gcgacctcca ccattctctg
     1441 cagatcctcg ggcaacgaat cccacgccgc tttgttgacg atggcttcca gggtggggcc
     1501 gggctcgtgc cagcccgggt agtagtagta gcgtgccacg tcctgaagcc ccatggccag
     1561 gtcgttgtag gggcccaccc attccgtggc gtcgatgacg cccgtctgca tggccgtgta
     1621 aatctccgac cccgccatgg acaccggcac gcccccggca cgcttgagca cctcgccgcc
     1681 gagccccggg atgcgcatct tcaatccctg aagatcctcc agggagttga tttccttgtt
     1741 gaaccagccc gccatctgca cgccgctgtt accggcggcg aggggcacga ggccgaacgg
     1801 ttcgtaggcc tcgcgccaca gctccatgcc gccgccgtag cgcagccagc cgttcatctc
     1861 ctgggccatc atgccgaaag gaaccgtggc gaacatggcg gctaccggga gcttgccgcg
     1921 ccagtagtag gcggctccat ggcccatctc cgccgtgccc tggcgtaccg tatcgaacac
     1981 ctcgaacgcg ccgaccagct ccccggcgcc gtagacccgg atgtctagcc gaccccgact
     2041 catgacccgc agattttcgg ccaggcgctc cggcgccatg ccgagcgccg gaaggttttt
     2101 cggccaggtc gtaatgagct tccagcgata gaccttatcg ggatcgggaa ccgcttcggg
     2161 cgccgtctgc tgcggcgcgc cgcagccggc gaggccaaac atgagcacca gggccaggat
     2221 ccgcatcacc atcatgtcgc tcttctccag tttcaatcga gggcctgcag gtttgcatag
     2281 cccagcatca accatttcgc gccggcgccg gcgaagttga cctgcacccg ggcgcgctca
     2341 ccggaaccct cggcctggag caccacgccc tcgccgaact tggcgtgtag cacccgctga
     2401 cccattcgca ggccgccctc agccccgact gcgtgcccgg cttctgcagg ccggccgtag
     2461 ggtcgctgca ccgcgccgcc catgcgcacc tcgtgaacga gctccgtcgg aatctccagc
     2521 aggaagcgcg acggccggtt gtaagtatcc gttccgtgca ggcgccgagt ctccgcatgg
     2581 gtgaggtaga gcgtctgctt ggcgcgggtg atgcctacgt agcacagccg cctttcctct
     2641 tccatgcgcc cgggctcttc ggcggacatg cggtggggga agaggccttc ctccatcccc
     2701 gcgagaaata ccatggggaa ctccagcccc ttggccgagt gcagggtcat catctgcacc
     2761 gcaggacccg cttccgcctg gcgatcaccc gcgtccagag ctacctggtc gagaaactcc
     2821 tcgagtatcg acgtctgctc ggcggcgtcg gccaggagag ggaatacctg ctcgccgctg
     2881 aactggcggc aggcgctgac cagctcctcc aggttgtcga cccgggcggc gccccgctcg
     2941 ccggcctcgc ggccgtggaa ttcgagcagc ccggtgcgcg tgagcatgcg ctcgcagagt
     3001 tcggccagct ccagcgactc
//
LOCUS       DBNHOX010000405         2474 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1767765, whole genome shotgun sequence.
ACCESSION   DBNHOX010000405 DBNHOX010000000
VERSION     DBNHOX010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1767765"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..336)
                     /locus_tag="AC16UZ_09015"
     CDS             complement(43..336)
                     /locus_tag="AC16UZ_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246862.1"
                     /translation="MKSQIDQFPKYFPGNSRSLLSNFDQHDIDSDSVRTGLHFLPSDR
                     LEINLTANYSTVDQAGPTRKTAGPGGLYYTPDAALLPNYENAIHDNLVEDPGF"
     gene            627..1934
                     /gene="aptA"
                     /locus_tag="AC16UZ_09020"
     CDS             627..1934
                     /gene="aptA"
                     /locus_tag="AC16UZ_09020"
                     /EC_number="2.6.1.-"
                     /EC_number="2.6.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     S-adenosyl-L-methionine:8-amino-7-oxononanoate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0016223 - beta-alanine:pyruvate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omega-aminotransferase AptA"
                     /protein_id="HIR6246863.1"
                     /translation="MSRADLENHWLPFTDNKTFKDDPRLVVRGEGAYLWNQRGEKLLD
                     GSAGLFTCALGHCRPEITEAVSAQLAELDYIPSFLRSHPRAFEAANLLTRILPDPINH
                     VFFCNSGSEAVDTAMKIVLQYQLARGQAQRSVFVTRDRAYHGVNLGGTALSGIARNRQ
                     AFGSLVPGIVHMRDTWDADQCFSRGQPARGGVDLADDLHRAVKTHGPNRIAACFVEPI
                     AGSTGVLVPPAGYLERLREICDEHDILLVFDEVITGFGRTGAAFGADSFGVTPDIMTM
                     AKALTNGAQPMGAVAVRDEIYQTVIDASPDDAVEFAHGYTASAHPAACAAAIATLGIY
                     EREKIFERAREMSEYFLDSVFALQNLPAVADIRGFGMLAGFDVAAEGTPGLRGMRLQR
                     ALFDAGLHVKTTGDAAILAPPLVIEKSQIDEMCETLRDVLKSI"
     gene            1984..>2474
                     /locus_tag="AC16UZ_09025"
     CDS             1984..>2474
                     /locus_tag="AC16UZ_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR6246864.1"
                     /translation="MSEEAEGVRVYRGLKGVHFDRSPTTYIDGRAGELRYWGYSIHEL
                     ADYSTFEETAYLLLHGELPTAAELAAFDGELKAARRLPDGLVDIIRAVQQAHPMDVLR
                     TAVSALAAFEPDVADTSRDGVLRKGIRLAAQVPAIVAAHHRLRSGLAPVEPDDDLGHA
                     AHFL"
BASE COUNT          411 a          760 c          837 g          466 t
ORIGIN      
        1 atcccagtcg atgcgccccg tgacgcccca gatgtcgctc tttcagaagc cggggtcctc
       61 caccaggttg tcgtggatcg cgttctcgta gttcggcagc agcgcagcgt cgggagtgta
      121 gtaaagcccg ccgggcccgg cggtcttgcg ggtcgggccg gcctggtcca ccgtgctgta
      181 gttggcggtc aggttgatct ccaggcggtc gctagggaga aagtgcagac cggtgcggac
      241 gctgtcggag tcaatgtcgt gctgatcgaa attgctcagc agcgatcggc tgttgcctgg
      301 aaagtacttc gggaactggt cgatctgcga cttcatgtag ccgtcacgtt tgcggctgga
      361 gaaggttgct gctatcgaac attgaacgtt gtttttagcg agccggtcct ccggctcctg
      421 tcgatgcggc gcactctgcc gggcttgacc ccgttcgtcc agttgccctg ttgcggtcct
      481 tgaagcggca ggttccgctg tacagaccag cgcaagggtc attatatttc gcggttcgat
      541 gggcctggct ggtgagtttc cgggggaagc gataagtgag cgaattactg aatcagtccg
      601 gggccggtgc tcggccaggt ccggcgttgt cgagagccga tctcgagaac cactggctgc
      661 cgttcacgga caacaagacg ttcaaggacg acccgcgtct ggtggtgcgg ggcgaaggcg
      721 cctacctgtg gaaccagcgg ggcgagaagc tgctggacgg ctccgcgggg ctgttcacct
      781 gcgcgctcgg tcactgccga cctgagatca cggaggcggt cagcgctcag ctggccgagc
      841 tcgattacat cccgtcgttc ctgcgcagcc atcctcgggc gttcgaagcg gccaacctgc
      901 tcacccggat cctgcctgac cccatcaacc atgttttctt ctgcaactcg ggttcggaag
      961 ccgtggatac ggccatgaag atcgtgctgc agtaccagct ggctagaggc caggcccagc
     1021 gtagcgtctt cgtcaccaga gatcgggcct accacggggt gaacctgggg ggcacggcgt
     1081 tgagcggcat cgcccgcaac cgtcaggcgt tcggatccct cgttccgggc atcgtgcaca
     1141 tgcgtgacac gtgggatgcg gaccagtgct tcagcagggg ccagccggcc cgtggcggtg
     1201 tggatcttgc cgacgatctg catcgagcgg tgaagaccca tggaccgaac cgcatcgccg
     1261 cctgcttcgt agagcccatc gcgggctcca ccggcgttct cgttccgcct gcgggttatc
     1321 tcgagcgcct gcgggagatc tgcgacgagc acgacatcct gctggtgttc gacgaggtga
     1381 tcaccgggtt cggccgaacg ggggcggcct ttggtgcgga ttccttcggc gtcacgccag
     1441 acatcatgac catggccaag gccctgacca acggtgccca gcccatgggc gccgtcgcgg
     1501 tgagagacga gatttaccag acggtgatcg atgccagtcc ggacgacgcg gtggagtttg
     1561 cccacggcta cacggcctcg gcccaccctg cggcctgcgc cgccgccata gcgaccctgg
     1621 ggatctacga gcgggagaag atcttcgagc gtgcccggga gatgtccgag tactttctgg
     1681 attcggtgtt cgccctgcag aacctgccgg cggtggcgga cattcgggga ttcggcatgc
     1741 tggccggctt cgacgtggcc gcggaaggca cgccgggact gcgtggcatg cgtctgcagc
     1801 gggcgctgtt cgacgcgggc ctgcatgtca agaccactgg ggacgcggcg atactggcgc
     1861 cgccgctcgt catcgagaag tcccagatcg acgagatgtg cgagacattg cgagacgtgc
     1921 tcaagtcgat ctgatgaggc caagcgggat cgcctggaca actcatgagc cgggggagga
     1981 cggatgagcg aagaagcgga aggcgtacgc gtataccgcg gcctgaaggg cgtgcacttt
     2041 gatcgctcgc caaccactta catcgacggg cgagcgggcg agctgcgcta ctggggttac
     2101 tccatacacg agctcgccga ttattcgacg ttcgaggaga cggcctacct gctgctccac
     2161 ggggagttgc ccaccgccgc cgagctggcc gccttcgatg gggagctcaa ggcggcgcga
     2221 cggcttcctg atggtctcgt cgacatcatc cgcgccgtgc agcaggcaca ccccatggac
     2281 gtgctccgca ctgccgtgtc ggcgctggca gcattcgaac cggacgtggc ggacacatcg
     2341 agggacggcg tcctgcgcaa aggcattcgg ctcgccgccc aggttccggc aatcgtggcc
     2401 gcgcaccatc gcctccgatc gggcttggca cccgtggagc ccgacgacga tcttggccat
     2461 gcggctcatt ttct
//
LOCUS       DBNHOX010000406         4107 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1126038, whole genome shotgun sequence.
ACCESSION   DBNHOX010000406 DBNHOX010000000
VERSION     DBNHOX010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1126038"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..901
                     /locus_tag="AC16UZ_09030"
     CDS             41..901
                     /locus_tag="AC16UZ_09030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6246865.1"
                     /translation="MRYESPASTKEAVALLAQEKGSAFVLAGGTDLLVRMKAELIEPD
                     LVVDIKRIPAMTDIRVTEKGFRIGAAVSAAQLGRHKALCKAWPGVVEAANLIGSDQIQ
                     GRCTVVGNLCNASPAADSVPALVAAGAKAVIVGRNGRRTVPVEKVITGPGRTSLEKGE
                     VIEALTLPKPASRTGDAYLRFTPRTEMDIAVVGVGISLALERGTVKKARVCLGAVAPT
                     PILVPAAARALIGSKLDDVALARLATACSAACNPIDDKRGTAAYRIRVAGVLARRAAT
                     IAFQRAGGKA"
     gene            898..1386
                     /locus_tag="AC16UZ_09035"
     CDS             898..1386
                     /locus_tag="AC16UZ_09035"
                     /inference="COORDINATES: protein motif:HMM:NF013923.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6246866.1"
                     /translation="MSNAVLVSTTINGDTVEFACPADEVLLDSLRDRVGLTGVKEGCG
                     TGDCGACSVTLDGELVCSCLVLGVEADGRAIGTVEGLTENGDLHPLQQNFIDHAALQC
                     GICTPGFLVAAKALLDRNPNPTETEIRYALAGNLCRCTGYDKIVRAVQASAKDLRKAK
                     KR"
     gene            1414..3699
                     /locus_tag="AC16UZ_09040"
     CDS             1414..3699
                     /locus_tag="AC16UZ_09040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6246867.1"
                     /translation="MAHDNSYTGQKFQVIGNRFARPDGVDKVTGRARYGADANAPGQL
                     VARVLRSPHAHARIKKIDTSKAEKLSGVKAVITSDDLPDLTGGNRGMTDILENCMARG
                     RALYDGHAVAAVAAIDEQTARKALKLIKVTYQMLPHVTDVDEAMQPEAPVIHDWVFTG
                     GVEPKPTKPSNVANRTEMGHGDVDAGFAAADVIVERSYKTEQTHQGYIEPHACLASVN
                     PDGSAELWVTTQGPFSYRNVCAGLLGLDIAKLKVTASEIGGGFGGKTHVWAEPLALAL
                     SRKANRPVKLVMTREEVFRATGPTASTSIDVKIGAKKNGEITAATLDMRYQGGAFPGI
                     WGMLGCMTGFACYDIKNQRSVGWDVIVNRPKVAAYRAPSAPMAAFAVESTMDIVAGEV
                     GMDAVDFRLKNVAREGSQAPYGPIFGPIGIESALQEVKKHPQMKARLKKNQGRGVACG
                     FWFNIGGQTCVDLNIGEDGTVNLAVGTVDVGGARSSLGMIAAEELGISYDDVKVHVAD
                     TASLGHNDTTEGSRGTFSSGMAAIFAARDAIEVLKDRAAQMLDVPRDAIVWEKGEARA
                     KGTQYSNLPKLSLREIAAKAARTGGPIAGHNEVTADGAGVAFAAHICDVEVDPETGLT
                     RVLRYTVIQDAGKAIHPDYVEGQFQGAAAQGIGWGLNEEYVYGEDGRLQNPGFLDYRI
                     PVCSDLPFIDTKILEVPNPNHPYGIRGVGEASIVPPLGAIGNAIANATGVRMHHVPMS
                     PVRVLDALDQAEASESQVSAG"
     gene            3689..3931
                     /locus_tag="AC16UZ_09045"
     CDS             3689..3931
                     /locus_tag="AC16UZ_09045"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR6246868.1"
                     /translation="MLVSLNGALRAAAGGQGSVNIEASTIRQLIERLVDRYPAMAGEV
                     DKGIAVAIDGVVYRDDWTQRIPENAEVFLMPRIAGG"
BASE COUNT          801 a         1296 c         1380 g          630 t
ORIGIN      
        1 cgtctgatcc ctcaccgact tcagccacag gacactcacg atgagatacg aatccccagc
       61 ctcgacgaaa gaagccgtcg cgctgctggc ccaggaaaaa ggctcagcct tcgtgctggc
      121 gggcggcacc gacctgctgg tcaggatgaa ggccgagctg atcgaacccg acctcgttgt
      181 cgacatcaaa cgcattccag caatgaccga catccgggtg accgagaagg ggtttcggat
      241 cggagcggcc gtttccgctg cccagctcgg ccggcacaaa gccctgtgca aggcgtggcc
      301 gggcgtggtg gaggcggcca atctcatcgg ctcggatcag attcaggggc gctgcacggt
      361 ggtcggaaac ctgtgcaacg catcgcccgc ggccgacagc gtgccggccc tggtcgccgc
      421 cggggcaaag gccgtcatcg tgggccggaa cggacgaaga acggtaccgg tggagaaggt
      481 gatcaccggg ccgggcagaa cctccctgga aaaaggcgag gtcatcgaag ccctcacgct
      541 cccgaaaccg gccagccgca cgggcgacgc ctacttgcgt ttcacccctc gaaccgagat
      601 ggatatcgcc gtggtgggcg tcgggatcag cctggcgctg gagcgaggca cggtcaaaaa
      661 agcgcgcgtc tgcctggggg cggtggcgcc cacgccgatt ctggtgccgg cggcggcaag
      721 agcactgatc ggatccaagc tcgacgacgt cgcgctggcc aggctggcca cagcatgctc
      781 cgcggcctgc aaccccatcg acgacaaacg cggcacggcg gcttaccgca tccgcgtggc
      841 gggagtgctg gcgcggcgcg cggcgacgat cgcgtttcaa cgcgcaggag gtaaggcatg
      901 agcaacgcgg ttttagtatc cacgaccatc aacggcgaca cggtcgagtt cgcctgcccg
      961 gcagacgagg tgctgctgga ctccctgcgc gaccgggtcg gtctgaccgg cgtcaaggag
     1021 ggctgcggga cgggcgactg cggtgcctgc agcgtgacgc tggacggtga gctggtgtgc
     1081 tcctgcctgg tgctcggcgt ggaagccgac ggccgcgcca tcggcacggt ggaaggtctc
     1141 accgagaacg gcgacctgca ccccttacag cagaacttca tcgatcatgc cgctctgcag
     1201 tgcggcatct gcactccggg gttcctcgtc gcggcgaaag cgctgctcga cagaaatccg
     1261 aacccgacgg agacggaaat tcgctatgcc ctggcgggca atctgtgccg ctgcaccggc
     1321 tacgacaaga tcgttcgcgc ggtgcaggcc tccgccaagg atctgcgcaa agccaagaaa
     1381 cgttaaccac tgacgcgacg ataggaattc gacatggccc acgacaacag ctacaccgga
     1441 cagaaattcc aggtgatcgg caaccggttc gcccggcccg acggcgtcga caaggtaacc
     1501 ggccgggctc gttacggtgc ggacgccaat gcaccgggtc aactggtcgc ccgggttctc
     1561 cgctcacctc atgcccacgc caggatcaag aagatcgaca cgtccaaggc ggagaaatta
     1621 tccggcgtca aagccgtcat cacgagcgac gatctgccgg acctgaccgg cgggaatcgc
     1681 gggatgacgg acatcctgga gaactgcatg gcccgcgggc gggcgctcta cgacggtcac
     1741 gcagtggccg cggtggccgc catagacgag cagacggccc gcaaggcgct gaagctgatc
     1801 aaggtgacct atcagatgct tccccacgtc accgacgtgg acgaagcgat gcaaccggag
     1861 gcgcccgtga ttcacgactg ggtgttcacc ggcggcgtgg agcccaagcc gacaaaaccc
     1921 tcgaacgtcg ccaaccgcac ggaaatgggc cacggcgacg tggatgccgg gttcgcggca
     1981 gccgacgtga tcgtggagcg gtcgtacaaa accgagcaga cccaccaggg ctacatcgag
     2041 ccccacgctt gcctggcatc ggtcaacccg gatggctcgg cggagctgtg ggtaaccacc
     2101 cagggaccgt tctcgtaccg gaacgtgtgc gccggcctgc tgggcctgga catcgccaag
     2161 ctgaaggtga cggcctcgga gatcggcggc ggcttcggcg gtaagaccca cgtctgggct
     2221 gagcccctgg ctctggccct ttcccgcaag gccaaccggc ccgtcaagct ggtgatgacc
     2281 cgcgaagagg tctttcgcgc caccggccca acggcgtcca cgtccatcga cgtgaagatc
     2341 ggcgccaaga agaacggcga gatcaccgca gcgaccctgg acatgcgcta tcagggcggc
     2401 gcgtttcccg gcatctgggg catgctggga tgcatgaccg gtttcgcctg ttacgacatc
     2461 aagaaccagc gatccgtggg ctgggacgtc atcgtcaacc ggccgaaggt ggccgcctac
     2521 cgggcgccct cggcacccat ggcggcgttc gcggtggaaa gcactatgga catcgtagcc
     2581 ggcgaggtcg gcatggatgc ggtggatttc cgtctcaaaa acgtcgccag ggagggctcg
     2641 caggcacctt acggtccgat cttcggtccc atcggcatcg agtccgcgtt gcaggaggtg
     2701 aagaaacacc cgcagatgaa ggcccgcctg aagaagaacc agggaagggg cgtcgcctgc
     2761 ggcttctggt tcaacatcgg tggtcagacc tgtgtggatc tcaacatcgg agaagacggc
     2821 accgtcaatc tggcagttgg aaccgtggac gtgggcggcg cgcgctcgtc cctgggaatg
     2881 atcgccgcgg aggaactggg aatttcctat gacgacgtca aggtgcacgt ggccgacacc
     2941 gcatcccttg gccacaacga caccacggaa ggcagccggg gcaccttctc ctcgggcatg
     3001 gcggctatat ttgccgcccg ggacgccatc gaggtgttga aggatcgcgc cgcgcagatg
     3061 ctggacgtgc cccgggacgc catcgtctgg gagaagggcg aggcgcgtgc aaagggtacc
     3121 caatacagca acctgcccaa gctctctctc agggagatcg ccgccaaggc cgcacgcacg
     3181 ggtggcccca tcgcgggcca caacgaggtg acggcggacg gggcgggtgt cgctttcgcc
     3241 gctcacatct gcgacgtcga ggtggatccc gagaccggac tgacccgtgt gctgcggtac
     3301 acggtcattc aggacgcggg caaggccatt catcccgact acgtggaggg ccagttccag
     3361 ggcgccgcgg cccagggcat tggctggggg ctaaacgagg aatacgtcta cggcgaagat
     3421 ggtcgcctgc agaatccggg cttcctggac taccggatcc ccgtctgctc agacctgccc
     3481 ttcatcgaca cgaagattct ggaagtcccg aaccccaacc atccctacgg tatccgtggg
     3541 gtgggtgaag cgtccatcgt tccaccgctg ggcgccatcg ggaacgcgat cgccaacgca
     3601 accggtgtgc gcatgcatca cgtgccgatg tcgccggtac gcgtgctcga cgccctggat
     3661 caggcagagg cgtcggaaag ccaggtcagt gctggttagc ctcaacgggg cgctgcgtgc
     3721 tgccgccggg ggccagggct cggtgaacat cgaggcgtca accattcggc agctgatcga
     3781 gaggttggtg gatcgctatc ccgccatggc cggagaggtg gacaaaggca tcgccgtcgc
     3841 catcgacggc gtggtctacc gtgacgactg gacccagcgg attccggaga acgccgaggt
     3901 ctttctcatg ccgcgcattg cgggtggttg agccagaaaa aaccgagcag aggtaagcca
     3961 tggctgactg ggacgagcgc aaacggcgcg cgagggaatc gtaccaacgc ttcgtaccgg
     4021 acgcagaccc cgagcgcgtg ctggcatcca tggagcgcag cctcggcgcg ctgggaacgt
     4081 ttgcttttgc tgcggtcggc gagatgt
//
LOCUS       DBNHOX010000407         5276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_803899, whole genome shotgun sequence.
ACCESSION   DBNHOX010000407 DBNHOX010000000
VERSION     DBNHOX010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_803899"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1740)
                     /locus_tag="AC16UZ_09050"
     CDS             complement(<1..1740)
                     /locus_tag="AC16UZ_09050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR6246869.1"
                     /translation="MTKRTIVNDFVIRFANVNGSGSASANGMFAKALFRMGIPVATRN
                     IFPSNIQGLPTWFEVRVSERGYLGRREGVDIMVAMNAETFEEDVASLNPGGYLVYDNT
                     KALPASLRRSDVHEVGIPISSMLLSEFSDPKQRSLFKNITYLGALTALLNIEFDVITG
                     MVSTQYKGKDALIGPNIHALELGRDYARDYLECPLPFQVKRADLNGDKIMVDGNTAAA
                     LGALYGGATVCAWYPITPSTSVAEEFERFANRMRIDGQSGQRKFAIIQAEDELSAIGI
                     VIGAAWNGARSFTATSGPGISLMTEFLGLAYFAEIPAVVFDIQRSGPSTGMPTRTQQG
                     DLISSAYASHGDTKHVLLFPSTPRECFDHAALALDLADRLQTPVIVMSDLELGMNDSI
                     SEPFEWDDERRYDRGKVLDREQLDSLERFGRYLDVDGDGIGYRTLPGTHPDKGAFFTR
                     GTSRDEFAAYTESPDVYVANMERLSAKWRTAARLVPSAEIESNGERAGIIYYGTSVAP
                     MREALDMLEEEGIRLDTMRITAFPFGAEVYDFIAAHDTVFVVDQNRDAQMKVLLVTEG
                     ELDPAGLVSVLYYG"
     gene            complement(1737..3521)
                     /locus_tag="AC16UZ_09055"
     CDS             complement(1737..3521)
                     /locus_tag="AC16UZ_09055"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6246870.1"
                     /translation="MKPTNILDPNYFHKVVDCQWACPAHTPVPEYIRLIAQQRYTEAY
                     MLNWDSNVFPGILGRTCDRPCEPACRRVRTEEKPVAICRLKRVAADHKGDISAYLPKV
                     PERKNGKRIALLGAGPASLAVARDLLPFGYEVHLFEKEPMGGGMMRSQIPAFRLPVGV
                     LEEEVDVILDMGVECHFGHEVSSMKEMLDEDFDAYFIGTGAPRGRDLELPGRAEVAEH
                     ISIGIDWLSSVAFGHTHSISGKVIVMGGGNTAMDCCRTAKRLGAESVSVVVRSAFEVM
                     KASEWEKEDAMSEGIPIINNRPPKEFVHENGKLKGVVFECVKPVKGDDGRLRYEPSGE
                     PDVFMEADHILMAIGQENHCPWIERDIGIQFDKWDCPMLDEETLQSTHPKVFFGGDSA
                     FGPENIIWASAHAHKAAISIHLMCQGKDLKADRPPEGVNLISQKMGVHEWSYDSEHDP
                     ARRYLVSHADKRLALADIKMEVELGFDEELGAKEAQRCLNCDVQTVFVDQLCIECDAC
                     VDICPMDCITFTENAEEPDLRRRLTAPAGNVEQDLYVSDELNTGRVMVKDEDVCVHCG
                     LCAERCPTNAWDMQKSIIHIPQLGSYAE"
     gene            3861..>5276
                     /locus_tag="AC16UZ_09060"
     CDS             3861..>5276
                     /locus_tag="AC16UZ_09060"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF037626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor family
                     protein"
                     /protein_id="HIR6246871.1"
                     /translation="MANELSPFQPPTPPAVGSRIAWNELHDGAAGLAVGRAAAAHEGL
                     TLMVAESTRQAQRWLDELRFFAPDVHLLHFPDGETLAYDAFSPHQDIISERLSTLRDL
                     PSQRTEREPTLLVVPVQTLLQRVAPRTYLDGHSLSLRTGQHFDLDAQRGRLNAAGYNA
                     AETVSAHGEYAVRGSLMDIYPMGADLPIRIDLFDDEVESLRTFDPDTQRTVEKIDAIR
                     LLPAKEFPLDGNAIARFRSNWHNTFNVDVRRCSVYQDISEGISPNGIEYYLAFFFEEL
                     ATLFDYLPANTVVIREAGVREAAERFLHDVGVRYESLRHDVERPILPPSDVYLRREEL
                     NAALKAWPQIVINDASARHAVDFATQACGDLEANPRLRDPARGLCEFLDGITQPVLFV
                     ADSAGRREVFLEFLNAAGIRPRETADLPAFRDSRLDCAIAVAPVEQGLWTDELVIVTE
                     SQVFGHQLEERRARSARVLDPE"
BASE COUNT          944 a         1726 c         1621 g          985 t
ORIGIN      
        1 gccgtagtaa aggaccgaga cgaggccggc gggatcgagc tcaccctcgg tcaccagcag
       61 aaccttcatc tgcgcatcgc gattctgatc cacgacgaaa accgtatcgt gggccgcgat
      121 gaaatcgtac acctccgcgc cgaaggggaa cgcggtgatg cgcatggtat ccaggcggat
      181 tccctcctcc tccagcatgt ccagggcctc gcgcatgggg gccacggagg ttccgtagta
      241 gataatgccc gccctttcgc cgttcgactc gatctccgcc gagggcacca gtctggccgc
      301 cgttcgccac tttgcgctca gccgctccat gttggcgacg tagacatccg gggattccgt
      361 gtacgcggcg aactcgtcac gcgacgtgcc cctggtgaag aatgctcctt tgtccggatg
      421 ggttccggga agcgtccggt acccgatgcc gtccccgtcc acatccaggt aacggccgaa
      481 ccgctccagc gaatccagct gttcccgatc cagcaccttg ccccggtcgt agcgacgttc
      541 gtcatcccac tcgaaaggct cggagatgct gtcgttcatc ccgagctcca gatcactcat
      601 gacgataacg ggcgtctgga gccggtcggc caggtctagc gccagcgcgg catggtcgaa
      661 gcactccctc ggcgtcgagg gaaacagcag cacgtgcttg gtgtccccgt gagaagcgta
      721 ggccgaggaa atcagatccc cctgctgcgt gcgggtgggc atgcccgtgg acgggccgga
      781 acgctggata tcgaacacga cggcgggaat ctcggcaaag taggcaagcc cgagaaactc
      841 cgtcatcagc gagatgcccg ggccgctggt cgcggtgaag gaacgggcgc cgttccacgc
      901 cgcaccgatg acgatgccga tggccgacag ctcgtcctct gcctgaatga tcgcgaactt
      961 gcgctggccg gattggccgt cgatacgcat tcggttggca aagcgctcga actcctccgc
     1021 caccgaggtc gatggcgtga tcgggtacca ggcgcacacc gtcgcgccgc cgtagagggc
     1081 tccgagcgcg gcggccgtgt tgccgtccac catgatcttg tcgccgttca agtcagcgcg
     1141 tttgacctgg aacggcaacg gacactccag atagtcccgg gcatagtccc ggccgagctc
     1201 gagcgcgtgg atgttcgggc cgatgagcgc gtctttgccc ttgtactggg tggacaccat
     1261 gccggtgatc acgtcgaact cgatgttcag gagcgccgtc agcgccccca ggtaggtgat
     1321 gttcttgaac aagctgcgct gcttcgggtc gctgaactcg ctgagcagca tgctggatat
     1381 gggaatcccc acctcgtgca cgtcgctgcg ccgaagcgat gccggcagcg ccttggtatt
     1441 gtcgtacacc aggtaaccgc cggggttcag gctcgcaacg tcttcttcga aggtctcggc
     1501 attcatggcg accatgatgt ccacaccttc gcggcgaccc aggtagccac gctcgctcac
     1561 cctgacctcg aaccaggtcg gcaggccctg aatgttcgac ggaaatatgt tccgcgtggc
     1621 aaccgggatg cccatccgga acagggcctt tgcaaacatg ccgttggcgc tggccgagcc
     1681 cgacccgttg acgttggcga aacgaatgac gaagtcgttg actatggtgc gtttggtcat
     1741 tctgcgtacg agcccagctg cggaatgtgg atgatggact tctgcatgtc ccaggcgttc
     1801 gtggggcagc gttccgcaca gagcccgcag tgcacgcaga cgtcctcgtc tttcaccatg
     1861 acacgaccgg tgttgagctc atcagatacg tagaggtcct gctcgacgtt gccggccggc
     1921 gcggtcagcc ggcggcgcag atcgggctcc tcggcgtttt ccgtgaaggt gatgcagtcc
     1981 atcgggcaga tgtccacgca cgcgtcgcac tcgatgcaca gctggtcgac aaacacggtc
     2041 tggacgtcgc agttgaggca gcgttgcgct tcctttgcgc cgagctcctc gtcgaacccg
     2101 agctcgacct ccatcttgat gtcggccagc gccagacgct tgtccgcatg ggacaccagg
     2161 tagcgccggg ccggatcgtg ctcgctgtcg tagctccact cgtgaacgcc cattttctgg
     2221 ctgatgaggt ttacgccttc cggcggacgg tcggccttga gatccttgcc ctggcacatg
     2281 agatggatgg agatcgccgc cttgtgagca tgggcagagg cccagatgat gttctccggc
     2341 ccgaacgccg agtcgccgcc aaagaagacc ttggggtgag tggactgcag cgtttcctcg
     2401 tccagcatgg ggcaatccca tttgtcgaac tggataccga tgtcccgctc gatccaggga
     2461 cagtggttct cctggccgat ggccatgagg atatggtccg cctccatgaa gacgtcgggc
     2521 tccccgctgg gctcgtagcg caggcgaccg tcgtcaccct taaccggctt gacgcactcg
     2581 aacaccacgc ccttgagctt gccgttttcg tgaacgaact ccttgggcgg ccgattgttg
     2641 atgatcggta tgccctcgct catggcgtct tctttttccc actccgaggc cttcatcacc
     2701 tcgaacgcgg aacgcaccac taccgagacc gactcggccc ccaggcgctt ggccgtgcgg
     2761 cagcaatcca tggcggtgtt gccgccgccc atgacgatga ccttgccgga aatcgagtgg
     2821 gtgtgcccga acgcgacgct ggagagccaa tcgatgccga tggagatatg ctcggctact
     2881 tccgctctgc ccggcagctc gagatctcga ccccgaggcg cgccggtacc aatgaagtag
     2941 gcgtcgaaat cctcatcgag catctctttc atgctcgaga cctcgtgacc gaagtggcac
     3001 tcgacgccca tgtcgagaat cacatcgacc tcttcttcga gcacgcccac cgggaggcga
     3061 aacgcgggta tctggctgcg catcatgccc ccgcccatgg gctctttctc gaacagatgc
     3121 acctcgtagc cgaaaggcaa cagatctctg gccaccgcca gggaagcagg gcccgcgccg
     3181 agcagggcaa tacgctttcc gtttttgcgc tccgggacct tcggcagata ggcgctgatg
     3241 tcgcccttgt ggtccgcggc gacgcgcttg aggcggcata tggcgacagg tttttcctcg
     3301 gtgcgcaccc gccggcacgc cggttcgcag ggacggtcgc aggtgcggcc aagaattcct
     3361 gggaacacgt tggagtccca gttgagcatg taggcttcgg tgtatcgctg ctgcgcgatc
     3421 agccggatgt actccggcac cggcgtgtgt gccgggcaag cccactgaca gtcaactacc
     3481 ttgtggaaat agttggggtc gaggatgttt gtaggtttca tgcgtacgac gactcgccgg
     3541 caggcatgca cggctacgcc tcccagctcc tttggttacg ctaatttgcg gcgacagggc
     3601 aaaccgttca tccggccgac tgctgggcca tccatatagc caggccgagc ctggacagtc
     3661 gcgaactcta accttatttt tcgtggcttg ccaaccaaat tggccgcctg gtgtgacacg
     3721 ttgcccagca cgtctgaggc cgcgtagcga ccagcgccgg cgtcagcgac gacctcgagg
     3781 cgtcggaagg gggaaaccaa ccggaaccgc caattcccga gtagaatacg cgccgctatg
     3841 gtcaaccgcc cagaactctg atggccaacg agctgtcgcc attccaaccg ccaacgccgc
     3901 cagccgtggg ttcccgcatc gcctggaacg aacttcacga cggtgctgcg gggcttgccg
     3961 taggccgcgc tgccgccgcc cacgaggggc tgacgctgat ggtcgccgag agcacgcggc
     4021 aggcccagcg ctggctggac gagcttcgat tcttcgcccc ggacgtccac ctgctgcatt
     4081 ttcccgacgg cgagaccctg gcttacgatg ccttctcgcc gcaccaggac atcatctccg
     4141 aacgtctgtc cacgctgcgc gacctgccgt cccagcgcac cgaacgggag cccaccctgc
     4201 tcgtcgtgcc tgtccagacc cttctccagc gggtagcccc gcggacctac ctcgacggcc
     4261 acagcctttc acttcgcacg ggccagcact tcgacctgga tgcgcaacgc ggccggctca
     4321 acgcggcggg gtacaacgcg gcagagacgg taagcgctca cggggagtac gccgtgcgcg
     4381 gttcgcttat ggacatctac cccatgggag cggatctgcc catccgtatc gacctgttcg
     4441 acgacgaggt ggaaagcctg cggacgttcg accccgacac ccagcgcacc gtcgagaaga
     4501 tagacgcgat tcggctgctg cccgcaaagg aatttccctt ggatggcaac gccatcgcac
     4561 gattccggag caactggcac aacacgttca acgtcgacgt gcggcgatgc agcgtctacc
     4621 aggacatcag cgagggcatc tccccaaacg gcatcgagta ctacctggcc ttcttcttcg
     4681 aagagttggc tacgctcttc gactacctgc cagccaacac cgtggtcatc cgggaggccg
     4741 gcgttcggga agccgccgag cggtttcttc acgacgttgg cgttcgctac gagagcctgc
     4801 gtcacgacgt ggagcgaccc attcttcccc cgtccgacgt ctacctgcgc agagaggagc
     4861 tcaacgccgc gttgaaggcc tggccgcaga tcgtcatcaa cgacgccagc gcccgtcacg
     4921 cggtcgactt cgcaactcag gcctgcggag atctggaagc caatccccgg ctgcgggacc
     4981 ccgcgcgcgg cctgtgcgag tttctcgacg gcatcaccca gccggtgctt ttcgtggccg
     5041 actcggccgg gcgccgcgag gtgttcctgg aattcctgaa cgcggccggc atacgtccgc
     5101 gagagaccgc cgacctgccc gcgttccgag attcgaggct cgactgcgca atcgccgtcg
     5161 cccccgtgga gcagggtctg tggaccgacg agctggtgat cgttaccgag agccaggttt
     5221 tcgggcatca gctcgaggag agacgcgccc ggagcgctcg cgtgctggac ccggaa
//
LOCUS       DBNHOX010000408         2799 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_483754, whole genome shotgun sequence.
ACCESSION   DBNHOX010000408 DBNHOX010000000
VERSION     DBNHOX010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_483754"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="AC16UZ_09065"
     CDS             complement(<1..1287)
                     /locus_tag="AC16UZ_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR6246872.1"
                     /translation="MPVLESSLDTRAEAFVRNKADMQQALEEFRALLDEAAEGGGAEA
                     AARLASRGKMPIRERISFVLDRDSPFLEISPLAAWRSAYAVGSGFVVGIGVIEGVECV
                     ILGHDPSVRAGAFNPFNSKKLMRGLEIARINRMPYVQFVESAGADLRGETGEDPEAAI
                     RREVDHFAESGRLFYEITELSKMRIPTISVVFGASTAGGAYQPGMSDYNIFIKNQSRV
                     FLGGPPLVKMATGEDANEESLGGADMHTQVSGLGDYLALDELDGIRMCREVVKHLNWR
                     KLGPEPRADFAEPLHDPEDLLGMVSKDLRSPFDMREVIARIVDASYFEEFKPLYGPTL
                     VCGWASIHGYPVGILGNNGALFSESSEKAAQFIQLCNQIDVPLLFLHNITGFIVGTDF
                     EQGGITKNGSKMINAVANSTVPHITVIVGASYGAGNY"
     gene            1402..2601
                     /locus_tag="AC16UZ_09070"
     CDS             1402..2601
                     /locus_tag="AC16UZ_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6246873.1"
                     /translation="MTTDQQLADFEHQVDGWFAENKPADPGFLLPETFMEVGTDQQFE
                     FLRDWQRKVYEAGYVGMAWPKAYGGGGLPQIYQDIATRAMARHRVPFLPNTIGLNWAG
                     PLILHMGSEEDKQRYIKGILSGDDIWCQGFSEPDHGSDLGNAQCRATRDGDEYVINGS
                     KIWTSLGTYAKYMILLARTATGGSSKYAGLSFFLCPMDAPGIEPRPIQKLTGEYGFCQ
                     TFFDEARIPATCLMGQEGDGWRIAMITLTFERGATGGQAGGLSAMDLNINDVVELARR
                     ARRDGGPALADPLIQDELVQLMIESRGNALMADRTTIPALASDWPEAIAMSGKLRATE
                     LKRRMCRFALAMQGGNGARYVSADAVDGGMWQRSYLNAFSGTIGGGTSEIQRNILGER
                     VLGLPKD"
     gene            2610..>2799
                     /locus_tag="AC16UZ_09075"
     CDS             2610..>2799
                     /locus_tag="AC16UZ_09075"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6246874.1"
                     /translation="MASIEFSDEQSLLLETAASFCRDRSPIERVRAGLGDENGCDAAV
                     WQEMVDLGWLGIAAPLEHG"
BASE COUNT          524 a          908 c          901 g          466 t
ORIGIN      
        1 gtaattgccc gccccgtacg aggcgccgac gatgacggtg atgtgcggaa ccgtggagtt
       61 ggcgacggca ttgatcatct tcgaaccgtt cttcgtgatg ccgccctgct cgaaatccgt
      121 gcccacgatg aagccggtga tgttgtggag gaacagcagc ggcacgtcga tctggttgca
      181 cagctgaatg aactgggccg ccttctccga agattccgag aagagcgcgc cgttgttgcc
      241 caggatgccg acggggtagc catgaatgct ggcccagcca cacaccaggg tgggcccgta
      301 caagggcttg aactcctcga agtaggaggc gtcgacgatg cgggcgatga cttcgcgcat
      361 gtcgaagggc gagcgcagat ccttggagac catgcccagc agatcctcgg gatcgtgaag
      421 gggctcggcg aagtcggctc tcggctcagg gccgagcttg cgccaattga gatgtttgac
      481 cacttcccgg cacatacgga tgccgtccag ctcgtcgagc gccaggtaat cgcccaaccc
      541 ggatacctgg gtgtgcatgt cagcgccgcc gaggctctcc tcgttcgcat cctcgccggt
      601 ggccatcttc accaggggcg gtccgccgag aaagacccgc gactggttct tgatgaagat
      661 gttgtagtca ctcattcccg gctggtaagc gcccccggcc gtggacgcgc caaagaccac
      721 cgatatggtg gggatgcgca tcttggaaag ctcagtgatc tcgtagaaca gccgcccgga
      781 ctccgcgaag tgatccacct cccggcggat ggccgcctcc gggtcctccc cggtctcgcc
      841 gcgcaggtcg gcacccgcag actccacgaa ctgcacatag ggcatgcgat tgatccgcgc
      901 gatctccagc ccgcgcatga gcttcttcga gttgaacggg ttgaaggcac cggcgcgcac
      961 ggaagggtcg tggccgagaa tgacgcactc cacgccctcg atgacgccga tgcccaccac
     1021 gaaaccggac ccgacggcgt acgcggagcg ccaggccgcc agcgggctga tctccagaaa
     1081 cggcgagtca cgatcgagaa cgaacgaaat ccgctcgcga atcggcatct ttccgcgtga
     1141 ggccagccga gccgcagctt ccgctccgcc gccctcggcg gcttcgtcca gcagagcccg
     1201 gaactcttcg agagcctgct gcatgtcggc cttgttgcgc acgaacgcct cggccctggt
     1261 gtccaggctc gattccaata ccggcatgcg gtccccccga tccgtcgagg ccactttatg
     1321 ccagaacttt ctcgtcagaa caaaacgtgc cgggagaggc gtttcgttgt gctagattga
     1381 gcccgcgacg ccggaggaga gatgacgacg gaccagcaac tcgccgattt cgagcaccag
     1441 gtggacggct ggtttgccga gaacaagccc gccgacccgg gctttctgct cccagagacc
     1501 ttcatggagg tcggcaccga tcagcagttc gagtttctcc gcgactggca gcgaaaagtc
     1561 tacgaggccg gatacgtggg gatggcctgg cctaaggcct acggcggggg cggcctcccg
     1621 cagatctacc aggacatcgc gacccgggcc atggcccgcc accgggttcc cttcctgccg
     1681 aacaccatcg gcctgaactg ggcagggccg ctgatccttc acatgggatc ggaagaggac
     1741 aagcagcgct acatcaaagg cattctcagc ggggacgaca tctggtgcca ggggttctcg
     1801 gaacccgacc acggctcgga tctgggcaac gcccagtgtc gggcgacgcg cgacggcgat
     1861 gagtacgtga tcaacggctc caagatctgg accagtctcg gcacctatgc caaatacatg
     1921 atcctgctcg cccgaacggc caccggcgga agcagcaagt acgcgggcct gagctttttc
     1981 ctttgcccga tggatgcgcc cggcatcgag ccgagaccca tacagaaact caccggcgag
     2041 tacggtttct gccagacgtt cttcgacgag gctcgaatac ccgccacctg cctgatgggg
     2101 caggaaggcg atggctggcg catcgccatg atcacgctca cgttcgagcg cggcgcgacg
     2161 ggcggccagg ccggcgggct gtccgccatg gatctcaaca tcaacgacgt ggtggagctg
     2221 gccaggcgcg ctcgtcgcga cggcggtccg gcgctggcgg atccgctgat tcaggacgag
     2281 ctggtgcagc tgatgatcga gtcccggggg aatgccctga tggcagaccg cacaaccatc
     2341 cccgcgctcg cctccgactg gcccgaagcc attgccatgt cgggcaagct gcgcgccacc
     2401 gagctcaaac gccgcatgtg ccgattcgcg ctggccatgc agggcggcaa cggcgcccgc
     2461 tacgtgagcg cggatgccgt ggatggcggc atgtggcagc gctcctacct gaacgcgttc
     2521 tcgggcacca tcggcggggg cacgtctgaa attcagcgca acatcctcgg agagcgggta
     2581 ctcggcctgc cgaaggattg agcaccaccg tggcgtccat cgaattctca gacgagcagt
     2641 cgctgctcct ggaaaccgcc gcctcgttct gtcgcgatcg atccccgata gagcgggtgc
     2701 gcgccggcct cggcgacgaa aacggctgcg acgccgccgt gtggcaggag atggtagacc
     2761 tcggctggct ggggatcgct gccccgctgg agcacggcg
//
LOCUS       DBNHOX010000409        11246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_740125, whole genome shotgun sequence.
ACCESSION   DBNHOX010000409 DBNHOX010000000
VERSION     DBNHOX010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11246
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_740125"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="AC16UZ_09080"
     CDS             complement(<1..224)
                     /locus_tag="AC16UZ_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6246875.1"
                     /translation="MNGPIAWFARNHIAANLLLAVMVLAGLLSLPIMPQKDFPDLDYK
                     LITVSVRYLGAAPEEVEDGVCIRIEEAIEGL"
     gene            complement(221..1384)
                     /locus_tag="AC16UZ_09085"
     CDS             complement(221..1384)
                     /locus_tag="AC16UZ_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6246876.1"
                     /translation="MNRRLQAALVLLGGFAVAVLVFLSGPKMQPQAPSAIPPLVRVIE
                     AEPREVQMTSHTHGTVVPRTESDLVPEVDGRVTAVSPSLVSGGFFNQGDVLLEIEPVD
                     YEVALEHARAGLARAESDLENARKSLTRIQDLVRRGATSDSDRDDAANRERIAQATLR
                     EARARLSRAERDLDRTRLVAPYQGRVRNERVDVGQFVKRGNPVATIYAVDFAEVRLPI
                     QDEELAFLDLPLGYTGDESSIPIPVTLRARFAGVEHEWQGEVVRTEGELDPATRMVHV
                     VARVPEPYVSRDGRPPLSVGLFVDADIQGKTLSDVVILPRSALRGGDRVLVVDQDERL
                     RFRPVDVYRVAEEEVYIVGGLARGERVCISPLQSTSDGMLVRVADVEQRAAPS"
     gene            complement(1381..2070)
                     /locus_tag="AC16UZ_09090"
     CDS             complement(1381..2070)
                     /locus_tag="AC16UZ_09090"
                     /inference="COORDINATES: protein motif:HMM:NF037008.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6246877.1"
                     /translation="MAQASRPLRADSAATRESILEASVKLFGERGYRNTSLRAITDAA
                     DVNLAAANYHFGSKAQLLDAAFERCIAPINDERMRRLRQLQASSSSPSVEALVRAFVD
                     LEFALTDNEHLDQFLAHLFAEPKSVSVPLLEKTFAPVVQAYFAALHAALPDVPVEVLR
                     WRFHLMIGSMIQLARFRHPLNFFQADSPPAEGSLQAGIDQLVEFVVAGLTQPGTSGDG
                     ASGDGASGDGE"
     gene            2219..2593
                     /locus_tag="AC16UZ_09095"
     CDS             2219..2593
                     /locus_tag="AC16UZ_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6246878.1"
                     /translation="MLGYATIGVNDMDRAVAFYDALLAELGAKQLMGMDRIKMYGTSP
                     DAGMLAICIPYDEEPQSPGNGNMVAISAGSREAVDRLYAKAMELGAADEGEPGERNPV
                     FYGAYVRDLDGNKLCFYEMKLG"
     gene            2690..3154
                     /locus_tag="AC16UZ_09100"
     CDS             2690..3154
                     /locus_tag="AC16UZ_09100"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6246879.1"
                     /translation="MFPRAVVASVDIQAPAREVWAVLVDFARYPEWNPFTPEVEAELA
                     VGAPVRLRVSMPGRRELERVEQINRVEPERALCWGMHMGHRALLCANRWQVLEPVRAA
                     TRYTTADRFSGLLVPLVMALYGGPMQLGFQQMADALKVRVEGTLPAGGAAGR"
     gene            3162..4343
                     /locus_tag="AC16UZ_09105"
     CDS             3162..4343
                     /locus_tag="AC16UZ_09105"
                     /inference="COORDINATES: protein motif:HMM:NF018452.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HIR6246880.1"
                     /translation="MRKLAVGLLGLFRRGALLWHKMRGRTLDDVAAERIVSGRSWEEF
                     CDTLKAAGAALTFPGAPRDPFNQAEGYRYLTRLTRAGLEAFVEYADAAAPVIHRVAHE
                     TVKLGSDNPDNYYQTARINGAWDYRITGRRNTIHYLSFGTQIGHYGQGGGMPPTGFID
                     DKDLEIDPDGSFTILLSPRRQPGNWLPMTEETGTLIVRQTYLDRAAEVPADLSIERVD
                     GGGTPGQVTPALMDAGLKDASTLVAGASLLFAKWARDFQSHTNRLPMFDQEVSNRAGG
                     DPNITYYHSHWTLAPDEALVIEALPPACEHWNFQLNNYWMESLDYRYHTIHTNKHLAT
                     YRPDGSVRIVVAHQDPGVPNWISTVGHASGTMSFRWIRAEATPEPTTRVVRLADVASL
                     A"
     gene            4336..5580
                     /locus_tag="AC16UZ_09110"
     CDS             4336..5580
                     /locus_tag="AC16UZ_09110"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6246881.1"
                     /translation="MPDFRPPRLPWPVRAFNRMGPALGATGLLPALDAEHMMAEARRR
                     AGLSDFGEDWFRAPLDALLGSINGEARLSALGRTIIRKRIVDALTTRLRARALLDAYP
                     EIESFDPGRVLVIAGLQRTGTTLLHRLLGADPRIRAVLSWEALNPVPLSGPAERERRI
                     RQALRAERGLKYIAPEFFAIHPVEHDMPEEDVLLLDVSFMSQSAEATMHVPTYARWLE
                     EHDHAPAYRYLRQLLSVLLWQRHAPSLVLKSPHHLEHLDTVLDVFPNATIIQTHRDPQ
                     QTVPSFASMVCHGASIFSDRVDVPRLTDHWLRKIRRVVERSLTARDRHGTARFVDVSY
                     YDLIGDPMAELARVYEAAGLAFDPQAQQGARETLRRQARDRFGRHRYRREDFGLTREL
                     IEGELGFYRRRFDIPFEEEAAS"
     gene            5577..6593
                     /locus_tag="AC16UZ_09115"
     CDS             5577..6593
                     /locus_tag="AC16UZ_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246882.1"
                     /translation="MNVKPTTDAPNAVGHRGVVSALVTALSDLVGRRDQLEPMLPDER
                     LDGRTYLVTGASSGLGKAAAVRLASAGAEVIMACRSGIPEAGDDVMRASGSGQVRMLE
                     VDLFDLESASAMCDRLRDDKQHLDGVVFNAGLIPLSARRGPQGFEEMFAVHFLSSRLI
                     VERLLADGVVAPSVDPATRPRLVFVSSEAHRSARPIDFEQFGAFVDYGIRGGMAEYGY
                     SKLHLCTYAVELARRLSSSDGPGVAVHALCPGPVASNIAREAPPWLKPVVGPIIRWLF
                     APSERAVEPVLLLAAGRAVEGRTGVYLHMMREKTFSSLAADPDNGRRLWEASGALIEP
                     FLRR"
     gene            complement(6583..8220)
                     /locus_tag="AC16UZ_09120"
     CDS             complement(6583..8220)
                     /locus_tag="AC16UZ_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007944983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6246883.1"
                     /translation="MPDNDDRISEPAADGELDVAIVGAGFSGLYMLHRVRDVLKLKTR
                     VFEAADGVGGTWYWNRYPGARCDSESYFYCYSFSKDLEQEWTWTSKFPEQPEILSYLN
                     HVADRFDLRRDIQLNTRVTGALFEDRSNRWIISTDTGETVSARFLISAVGCLSTRNLP
                     DIPGIESFAGEHYHTGNWPHEGVDFRGKRVGLIGTGSTGIQAAPVIAEEAEHLYVFQR
                     TPNFSVPARNTPLTAERTAEIKANYDEIRRISRTSGFGFPYETPEHAAMEVTPEERQC
                     VYEQRWHEGGTSFGFRSFSDILSDQAANDSASDFIRSKIRETVKDPEVAELLIPHDHP
                     YGTKRPPLDTNYFETYNRDNVTLVDVRSAPIKEITPRGVRTARDEYELDVIVFATGFD
                     AMTGALLAMNIEGSGGLRLSDKWANGPVTYLGLQVAGFPNLFTITGPGSPSVLANMPV
                     AIEQHVEWVSDCLEHLYANGISRIETTQAAEKGWVRHVAEEGDATLFPLANSWYVGAN
                     IPGKTRVFMPYVGGMGKYRDHCDRSAKHGYEGFVLSA"
     gene            8344..10362
                     /locus_tag="AC16UZ_09125"
     CDS             8344..10362
                     /locus_tag="AC16UZ_09125"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6246884.1"
                     /translation="MSGNDFVPKQAAAGLLLLAVVGCGSEEAPPADAGREQALPAGTN
                     SGHLVAYSEVREPCANADPLRIPLFGDVHVHTSFSFDAAANSVGTTPADAHKFALGEP
                     IPFFPVDASGTPTGTIRIDRPLDFLAVTDHGEFLGERSLCRDPRSPKYSSAFCEEYRT
                     GERHGMRMLGRVITSETPRRVPELCGDDGALCLEYAEGPWRRIVEAAEAAYDRSPGCT
                     FTSFVGYEYTGTPGTSNYHRNVIFRNASVPPRPVSYIDAPVDSLLWNALDAACGAVDG
                     CDYLTIPHNSNLANGRMGPYRGIELTEANRRAYAEQRQRREPVMEMFQHKGASECING
                     LTTVLGEPDELCDVEAVRVIGRAETYQTARMVDGELVLGETTEVTQECAPGESSSNGM
                     LGAGCVSATDYMRSGLLVGLAEEQEIGVNPIKLGMIAATDTHTSTPGAVAEGDWRGHV
                     TGEATPAERLEPGLLTSGIDGNPGGLAGVWAVENSRDAIFDAMLRREVFGTSGPRIVP
                     RFFAGWGYDAEMCGSADLVARGYGGGVPMGGDLPRGAPGDRPAFIAWAARDPAPSAQP
                     LESLQLVKGWIDAEGQARNRVIPIAGGEGQVARDTLCAVYRDDDFDPAVPAYYYLRVV
                     EPPTPRWSTFDCQRLPESDRPAVCSDGSYPQTIREMAWTSPIWYRPEG"
     gene            10370..10795
                     /locus_tag="AC16UZ_09130"
     CDS             10370..10795
                     /locus_tag="AC16UZ_09130"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA];
                     GO_process: GO:0045947 - negative regulation of
                     translational initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HIR6246885.1"
                     /translation="MPRVERSIEIAAPVSRVQQVIVDFERYPEFVPFMERAELLGIEG
                     ERRRVRFTLNLVARISYTLDLVPGTDSGLTWTLVDGPFVRNNGSWRLKATNDDGTLAT
                     YTADVELKGFLPGFLERRLVAESLPATLRAFRDRAESDA"
     gene            complement(10856..>11246)
                     /locus_tag="AC16UZ_09135"
     CDS             complement(10856..>11246)
                     /locus_tag="AC16UZ_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6246886.1"
                     /translation="FHYTLFPNFAVSIWADGFHFLRARPHPTDPERCVFDNWWYASQP
                     EGETELVSTMIGPVDRDAVVEHEVFDYGEQTMGMTIDQDMAVFIRQQKGFRSRGFKGV
                     YLSKQESRVRRYHELIDDYIEGRLPKR"
BASE COUNT         1850 a         3664 c         3777 g         1955 t
ORIGIN      
        1 aggccctcga tggcttcctc gatgcggatg cacacgccgt cctcgacctc ttcgggagcg
       61 gcacccaggt agcgcaccga caccgtgatc agtttgtagt ccaggtccgg gaagtccttt
      121 tgaggcatga tcggcaggga tagcaggccg gccagaacca tgaccgcaag cagaaggttg
      181 gctgcgatgt ggttgcgggc aaaccaggcg atggggccgt tcatgagggc gcggctcgct
      241 gctcgacatc ggcaacgcga accagcatgc catcgctggt gctctgcagc ggcgagatgc
      301 agacccgctc gccccgggcc aggccgccga cgatgtagac ctcctcctct gccacccggt
      361 agacgtcaac gggcctgaag cgcagcctct cgtcctgatc cacgacgagc acccggtcgc
      421 ctcccctcag tgccgatcgg ggcagaatga ccacgtcact gagcgttttg ccttgaatgt
      481 ccgcatcgac gaagagcccg accgacagag gcggccgccc gtcccggctg acgtagggct
      541 cgggaactct tgcgactacg tgcaccatgc gggtcgccgg gtccagctca ccttccgtac
      601 gcacgacctc gccctgccac tcatgctcca cgccggcgaa acgggcacgc agggtcaccg
      661 gtatggggat cgaagactcg tcgcccgtat acccgagcgg cagatcgaga aacgcgagct
      721 cctcgtcttg aatgggtagc cggacctccg cgaagtccac ggcgtagatc gtcgccaccg
      781 ggttgccccg cttgacgaac tggccgacgt ccaccctttc gttgcgaacc cgcccctggt
      841 agggcgcgac caggcgggtc ctgtccaggt cgcgttccgc tctggagagc cgcgcccgcg
      901 cttcgcgcag cgtggcctga gcaatgcgtt cccggtttgc cgcgtcgtcc cgatccgagt
      961 cgctggtcgc tccgcggcgg acgagatcct gaatgcgggt cagtgacttc ctggcgttct
     1021 ccagatcact ctctgccctg gccaaacctg cccgggcatg ctcgagggcc acctcgtagt
     1081 cgacgggttc gatctccagc agcacgtcgc cctggttgaa gaaaccgccg gagacgaggg
     1141 acggcgagac tgccgtgaca cggccgtcca cctccggcac cagatcgctt tccgtgcgcg
     1201 ggaccaccgt gccgtgggtg tggctcgtca tctgcacctc acggggctcg gcctcgatga
     1261 cccgtacgag cggaggaatg gcgcttggcg cctggggctg cattttcggc ccgctcagaa
     1321 acaccagcac ggccacggca aagcccccta ggagcaccag cgcggcctga agccttcgat
     1381 tcattcaccg tctccgctgg caccgtctcc gctggcaccg tctccgctgg ttcccggctg
     1441 ggtgagcccc gcaacgacga actccacgag ctgatcgatg cctgcttgca gggagccttc
     1501 tgcggggggc gagtctgcct gaaagaagtt caatgggtgt ctgaaccgcg ccagctggat
     1561 catcgatccg atcatgaggt gaaagcgcca gcgcaatacc tccacgggga cgtcgggcag
     1621 cgcggcgtgc agggcggcga agtaggcctg cacaaccggc gcgaaggtct tctcgagcag
     1681 cggcacggag acggatttcg gttccgcgaa cagatgggcg aggaactggt cgaggtgctc
     1741 gttgtccgtg agcgcgaatt ccagatcgac gaaggcgcga acgagcgctt ccaccgaagg
     1801 cgagctggac gacgcctgaa gctggcgcag gcgccgcatg cgctcgtcgt tgattggcgc
     1861 gatgcagcgc tcgaacgctg catcgagcag ctgagccttt gacccgaagt gataattggc
     1921 ggcggccagg ttcacgtcgg cggcgtcggt aatggccctc agcgacgtgt tgcggtagcc
     1981 gcgctcgccg aaaagcttca ccgaggcttc gagaatactt tcccgggtgg ctgccgagtc
     2041 tgcgcgcaac ggtctggatg cctgagccac gcggcattcc tagttcaaac ggtcgtttaa
     2101 attagcacgc ggtgctcggg actgtcacgc gctgattgcg aactcggctg gcacgcgggg
     2161 cgcggcggcg cgacaatgcc gctcgctatc tctcaacgag ccaaaaggag ggcctctcat
     2221 gctgggctac gcaaccattg gggtgaatga catggatcgg gcggtcgcct tctacgatgc
     2281 gctgctcgcg gagctgggtg caaagcagct catgggaatg gaccgcatca agatgtacgg
     2341 aaccagtccg gatgccggca tgctggccat ctgcatccct tacgatgaag aacctcaaag
     2401 ccccggcaac ggcaacatgg tggccatatc cgccggatcc cgggaggccg tggatcggct
     2461 ctacgccaag gcgatggagc tcggtgctgc cgacgaggga gaacccggcg agcgcaatcc
     2521 cgtcttctat ggcgcctacg tcagggatct ggacggcaac aagctctgct tctacgagat
     2581 gaagctgggg tagccgccag gcggcgattc ggtcatgcga tccgctcagg cggcgcccgc
     2641 gacggacggt gaaagcgagc gacttcatcg gcggtaccgg caggggcgcg tgtttcctcg
     2701 cgccgtggtt gccagcgtgg acatccaggc gccggcccgg gaggtctggg cagtgctggt
     2761 ggattttgcc cgctatccgg aatggaaccc gttcacgcct gaggtagagg cggagctggc
     2821 tgtgggcgcg ccggttcgcc tgcgggtgag catgccgggt cggcgcgagc tagagcgggt
     2881 ggagcagatc aatcgggtgg aaccggagcg cgcgctctgc tggggcatgc acatgggtca
     2941 tcgggccctg ctgtgcgcca accgctggca ggttctcgag ccggtgaggg cggccacgcg
     3001 atacaccacc gcggaccggt tcagcggtct cctggtgccg ctggtcatgg cgctctacgg
     3061 gggcccgatg cagcttggct tccagcagat ggccgacgcg ctgaaggtgc gtgtagaagg
     3121 cacgctgccc gccggcggag ctgccggccg atgaggagat catgagaaaa ctcgcggtgg
     3181 ggttgctcgg cctgtttcgc aggggtgcgc tgctctggca caagatgcgc gggcggaccc
     3241 tggacgacgt ggctgccgag cgcatcgtca gcggccgttc ctgggaggag ttctgcgaca
     3301 ccctcaaggc cgctggcgcg gcgctgacct ttccgggcgc tccgcgcgat cccttcaacc
     3361 aggccgaagg ctaccgctac ctcacccggc tgacccgggc ggggctcgaa gctttcgtgg
     3421 agtatgccga cgccgcggcg ccggtcatcc atcgcgtcgc ccacgagacg gtcaagctcg
     3481 gctccgacaa tcccgacaac tactaccaga cggcgcgcat caacggcgcc tgggattacc
     3541 gcatcaccgg caggcgaaac acgatccact acctgagctt cggcacccag atcggccact
     3601 acggccaggg gggcggcatg ccgccaacgg gcttcatcga cgacaaagac ctggagatcg
     3661 atccggacgg ctcgttcacc atcttgctca gccctcgccg gcagcccggt aactggttac
     3721 ccatgaccga agagacggga accctcatcg tccgtcagac ctacctcgac cgtgcggccg
     3781 aggtgcccgc cgatctgtct atcgagcggg tggacggcgg cggcacgccg ggtcaggtga
     3841 ccccggcact gatggacgcg ggcctcaagg atgcgagcac gctggtggca ggcgcatcgc
     3901 tcctgttcgc caagtgggcg agggatttcc agtcccacac caaccggttg cccatgttcg
     3961 atcaggaggt ctccaaccgg gcgggcgggg accccaacat aacttactat cacagtcact
     4021 ggacgctcgc gcccgacgag gcgctggtca tcgaggccct gccgccggcc tgcgagcact
     4081 ggaacttcca gctgaacaac tactggatgg aaagcctcga ctaccgctac cacacgattc
     4141 acacgaacaa gcacctggcc acctaccgcc cggacggctc ggtgcggatc gtggtcgctc
     4201 accaggatcc gggggttccg aactggatca gcaccgtggg tcacgcttcg ggaaccatga
     4261 gcttccgctg gattcgggcg gaggcgacgc cggagccgac cacccgggtc gtcaggctag
     4321 ccgacgtggc gagccttgcc tgacttccgc cctccgcggc tcccgtggcc cgtgcgcgcc
     4381 ttcaaccgga tgggccccgc gcttggcgcg acggggctcc tgccagcatt ggatgccgag
     4441 cacatgatgg cggaagcgcg tcggcgcgcg ggcctttcgg actttggcga agactggttc
     4501 cgcgcgccgc tggacgccct gttgggctcg atcaacggcg aggcccggct tagcgctctg
     4561 ggcagaacca tcatccgcaa gcgcatcgtc gacgctctca ccacgcgtct gcgcgcccgg
     4621 gcgctcctgg acgcgtatcc ggaaattgag tccttcgacc cgggcagggt gctggtcatc
     4681 gccgggctgc agcgcacggg aaccaccctt ctgcatcgcc tcctcggcgc cgatccgcgc
     4741 atccgggcgg tgctctcgtg ggaagcgctc aacccggtgc ccctgagcgg cccggcagag
     4801 cgtgagcgca ggatccgcca ggccctgcgc gccgaacggg gactgaagta catcgcgccg
     4861 gagttctttg ccattcaccc cgtcgagcac gacatgcccg aggaagacgt gctgctgctc
     4921 gatgtctcgt tcatgagcca gtccgccgag gcgaccatgc acgttcccac ctacgctcgc
     4981 tggctggagg agcacgacca cgcgcccgcc tatcggtatc tcaggcagct tctgagcgtg
     5041 ctgctgtggc agcgccacgc acccagcctg gtgctcaagt cacctcacca tctggagcat
     5101 ctggataccg tgctcgacgt ttttccgaac gcgacgatca ttcaaaccca ccgcgacccg
     5161 cagcagacgg tgccgtcgtt tgccagcatg gtctgtcacg gggcttcgat attcagcgat
     5221 cgggtggacg tccccaggct caccgatcac tggctccgca agatccgtcg ggtggtggag
     5281 cgctccctga cggcccggga tcgacacggg acggcgcgct tcgtggacgt ctcctactac
     5341 gacctcatcg gcgatccgat ggctgaactc gctcgagtct atgaggctgc tggcctggcc
     5401 tttgaccctc aggcgcagca gggtgcccgc gagacgttgc gccggcaggc ccgcgaccgc
     5461 ttcggccggc atcgctatcg ccgtgaggac ttcggtctga cccgggaact gatcgagggc
     5521 gagctggggt tctaccgcag gcgtttcgac atccccttcg aggaagaggc cgcatcatga
     5581 acgtcaagcc gaccacggac gcgcccaacg ccgtcgggca ccgcggcgtc gtgagcgcgc
     5641 ttgtcacggc gctgagcgat ctggtcggac gaagagatca gctcgagccg atgctgcctg
     5701 acgagcggct ggacggtcgg acatatctgg tcaccggcgc cagcagcggc ctgggcaaag
     5761 cggcggccgt gcgcctggcg agcgcgggcg cggaagtgat catggcctgc cggagcggga
     5821 ttcccgaagc cggcgacgac gtcatgcgcg cgtcgggcag cgggcaggtc aggatgctgg
     5881 aggtggatct tttcgacctc gagagcgcct cggccatgtg cgaccgtctt cgggatgaca
     5941 agcagcacct cgacggtgtc gttttcaacg ccgggctcat accgctttct gcgcggcgcg
     6001 ggccccaggg cttcgaggag atgttcgccg tgcactttct gtccagccgg ctcatcgtcg
     6061 agcggctgct ggcggacggt gtggttgcgc cgagtgtcga cccggcgacc cggccacggc
     6121 tcgtgttcgt ctcctccgag gcgcatcggt ctgccaggcc catcgacttc gagcagtttg
     6181 gcgcgttcgt cgactacgga attcgcggcg gcatggccga gtacggctac agcaaactgc
     6241 atctgtgcac gtatgccgtg gagctggccc gcaggctgag ctcgagcgac gggccggggg
     6301 tagccgtgca cgccctgtgc ccgggcccgg tggcctcgaa catcgccagg gaagcgccgc
     6361 cctggttgaa gccggtggtt gggcctatca tccgatggct gttcgccccg tccgagcgcg
     6421 cggtggagcc tgtgcttctg ctcgccgccg ggcgtgcagt ggagggtcgt accggcgttt
     6481 accttcacat gatgcgcgaa aagaccttct catcgctggc cgccgatccc gataacggcc
     6541 gccgcctgtg ggaggcgagc ggcgccctga tcgagccgtt cctcaggcgc tgaggacgaa
     6601 tccctcgtag ccgtgcttgg ctgagcggtc gcagtgatcg cggtatttgc ccatgccgcc
     6661 cacgtaaggc atgaaaacgc gggttttgcc cgggatgttc gcgccgacgt accaggagtt
     6721 ggccagtgga aacaacgtcg cgtcgccttc ctcggcgacg tgccggaccc agcccttctc
     6781 ggcggcctgc gtcgtctcga tgcgtgagat gccgttcgcg tagagatgct cgaggcagtc
     6841 agacacccac tcgacgtgct gctcgatggc caccggcatg ttggccagaa ccgaagggct
     6901 gccgggcccg gtgatggtga acaggttcgg aaatccggcc acctgcagac ccaggtaggt
     6961 gaccggtccg ttggcccact tgtcagaaag acgcagcccc ccggatccct cgatgttcat
     7021 tgcgaggagt gccccggtca tcgcgtcgaa gccggtggcg aagacgatga cgtcgagctc
     7081 gtactcatcc cgtgcggtcc tgaccccgcg gggagtgatc tccttgatgg gggccgatcg
     7141 gacgtcgacc agggtgacgt tgtcgcggtt gtaggtctcg aagtagttcg tatccagtgg
     7201 tggacgtttg gtgccgtagg ggtgatcgtg gggaatgagg agctccgcga cctcagggtc
     7261 cttcacggtc tcacggatct tgctccggat gaagtccgac gcgctgtcgt tggccgcctg
     7321 gtcagacaga atgtcgctga acgaccgaaa gccgaagctg gtacctccct cgtgccaccg
     7381 ctgctcgtag acgcactgac gctcctcggg tgtaacctcc atggctgcgt gttccggcgt
     7441 ctcgtaagga aagccgaaac ccgaagtgcg cgagatccgt cggatctcgt cgtagttggc
     7501 cttgatctct gccgttcgct cggccgtcag cggggtgttg cgggcgggga cgctgaaatt
     7561 cggcgtgcgc tgaaagacgt agaggtgctc ggcttcctcg gcgatcaccg gtgccgcctg
     7621 gattccggtg gagcccgtgc cgatgaggcc cacgcgcttt ccccggaagt cgacgccttc
     7681 atgaggccag ttgcccgtgt ggtagtgctc gccggcaaag ctctcgatgc cgggaatatc
     7741 cggcaggttc cgggtcgaga ggcaacccac agcgctgatc agaaagcggg cggacacggt
     7801 ctcgccggtg tcggtggaga ttatccagcg gttgctccga tcttcgaaaa gggcgccggt
     7861 gactcgtgtg ttgagctgga tgtcacggcg cagatcgaac cggtctgcca cgtgattgag
     7921 gtagctgaga atttccggtt gctcggggaa cttgctcgtc cacgtccact cctgctcgag
     7981 atccttggag aacgagtagc agtagaagta gctttccgaa tcgcagcggg cacctgggta
     8041 ccggttccag taccacgtgc cgccgacccc gtccgccgcc tcgaagacgc gcgtcttcag
     8101 cttcagcacg tcgcgcaccc ggtgcagcat gtagaggccg gaaaagcccg cgccgacgat
     8161 ggcaacgtcg agctcgccgt ccgccgctgg ctcggaaatc ctgtcgtcgt tgtctggcat
     8221 gcttctctcc ctccgacctt ctttctagtt tgccggtttc gccgtgacct gcaagtcgtg
     8281 accggcgcgg ccgatcggct ccatgctaag gttgacggtc gcgaggatcg ggagggattt
     8341 gacgtgagcg gaaacgattt cgttcccaag caggcggcag ccggcttgct gctgctcgcc
     8401 gtcgtcggct gcggaagtga agaggcgccc ccggcggacg ccgggcggga acaggcgttg
     8461 cctgccggca cgaactcggg gcatctcgtt gcctacagcg aagtgcgcga gccttgcgcc
     8521 aacgccgatc cgctgcgcat acccctgttc ggcgacgttc acgttcacac cagcttctcg
     8581 ttcgacgccg cggcgaacag cgtaggtacc acgcccgcgg atgcgcacaa gttcgcgctt
     8641 ggcgagccca tacccttctt tccggtagac gcgagcggca cgccgacggg caccatccgc
     8701 atcgatcggc cgctggattt cctcgcggtg accgaccacg gggagttcct gggcgagcgg
     8761 tctctgtgcc gggacccgcg ctcgccgaag tactcgtccg ctttctgcga ggagtaccgg
     8821 actggcgagc gacacggcat gcgcatgctg ggcagggtaa tcacctcgga gacgccgcga
     8881 cgggtgcccg agctgtgtgg agacgacggc gcgctgtgtc tggaatacgc tgagggtccg
     8941 tggcgccgga tcgtcgaggc cgctgaggcc gcctacgatc gctcgcccgg ctgcacgttc
     9001 accagcttcg tcggctacga gtacacgggc acgcctggca cctcgaacta ccaccgcaac
     9061 gtaatcttcc gcaatgccag cgtcccgccg cggccggtgt cttacatcga tgcgcccgtg
     9121 gacagtctgc tctggaacgc gctggatgcg gcctgcggtg ccgtcgatgg ttgtgactat
     9181 ctgaccattc cgcacaactc gaacctggcc aacggacgga tgggccctta tcggggcatc
     9241 gagctcaccg aggcgaatcg gcgtgcttac gcggagcagc gtcagcgccg ggagcccgtc
     9301 atggagatgt tccagcacaa gggggcatcg gagtgcatca acgggctgac caccgtgctc
     9361 ggcgagccgg acgagctgtg cgatgtggag gccgtccggg tgatcggccg cgcggagacg
     9421 taccagacgg cgcggatggt cgacggtgag ctggtgctgg gggagaccac cgaggtcacc
     9481 caggaatgcg cgcccgggga gtcttccagc aacggcatgc tgggcgccgg ctgcgtgtct
     9541 gccacggatt acatgcgctc cgggctcctg gtaggcctgg ccgaggagca ggaaatcggg
     9601 gtcaatccca taaagctcgg catgatcgcc gccacggaca cccacacctc cactcccggg
     9661 gccgtggcgg aaggcgactg gcgcggccac gtgaccggcg aagcgacgcc ggccgagcgc
     9721 ctcgagccgg ggctgctgac cagcgggatc gacggcaatc ctggcggcct ggcaggcgtc
     9781 tgggccgtgg aaaattcgcg cgatgccata ttcgacgcca tgctccggcg ggaggtgttc
     9841 ggcacctccg ggccccgcat cgtgccgcgc ttttttgcgg gctggggcta cgacgccgag
     9901 atgtgcggca gcgccgacct ggtggctcga ggctatgggg gcggggtgcc catgggcggc
     9961 gatctccccc ggggtgcccc gggtgaccgg cccgctttca tcgcctgggc ggcgcgggat
    10021 ccggcgccct cggcccagcc gctcgagtcg ctgcagctgg tcaaaggatg gatcgacgcc
    10081 gagggtcagg cgcgcaaccg ggtcattccc attgctggcg gagaggggca ggtagcgcgg
    10141 gacacccttt gcgccgttta tcgcgatgac gacttcgatc ccgctgtgcc cgcgtattac
    10201 tacctgcgcg tggtggagcc gccgacgccg cgttggagca ccttcgactg tcagcgtctg
    10261 cctgaatccg accggccggc cgtgtgcagc gacggctcgt atccccagac gatccgggaa
    10321 atggcctgga cgtctcccat ctggtaccgg cccgagggct gagcggcaga tgcccagggt
    10381 cgagcgcagc atcgagatcg cggcgcctgt atcgcgcgtg cagcaggtca tcgtcgactt
    10441 cgaacgctat ccggagttcg ttcccttcat ggaacgcgcg gagctgctgg gtatcgaggg
    10501 cgaacgacgc cgcgttcgct tcacgctgaa tctggtagcg cggatcagct ataccctgga
    10561 cctcgtgccc ggcaccgatt cgggcctgac atggaccctg gtagacgggc ccttcgtgcg
    10621 caacaacggc agctggcgtc ttaaggccac gaacgacgac gggaccctgg cgacctacac
    10681 cgccgacgtc gagctcaagg gttttctgcc gggattcttg gaacgacggc tcgtcgccga
    10741 aagccttccc gccaccctgc gcgcgttccg cgacagagcc gagtcagacg cctaggcagc
    10801 cggtcgcccc gcggctgccg cccccggagg gttcggcttc ttcaggccgc ctggatcagc
    10861 gcttgggcag gcgcccctcg atgtagtcgt cgatgagctc gtgatagcgg cggacccgac
    10921 tctcctgctt ggacaggtag acgcccttga aaccccggga ccggaatccc ttctgctggc
    10981 ggatgaacac ggccatgtcc tggtcgatgg tcatgcccat ggtctgctcg ccgtagtcga
    11041 acacctcgtg ctccaccacg gcgtctcggt ccaccgggcc gatcatggtg ctcacgagct
    11101 ccgtctcgcc ctcgggctgg ctggcgtacc accagttgtc gaagacgcac cgttccggat
    11161 cggtgggatg gggccgcgcc cgcagaaagt gaaagccgtc ggcccagatc gacaccgcga
    11221 agttcggaaa cagcgtgtag tgaaac
//
LOCUS       DBNHOX010000410         2173 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1126478, whole genome shotgun sequence.
ACCESSION   DBNHOX010000410 DBNHOX010000000
VERSION     DBNHOX010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1126478"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="AC16UZ_09140"
     CDS             <1..680
                     /locus_tag="AC16UZ_09140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6246887.1"
                     /translation="PVVHGGTYAELVTVPAGFVAPAPANLDDVETSTIPLAGLTVWQS
                     LFEFAGLTAGQTILIHAAAGGVGSLAVQLAAHAGARVFATTSAANAAYVRELGAHRII
                     DYRQENLGEVMGSEVPAGFDVVFDTVGGEVLGESYDLVRPGGALPALNDAPDEALCAQ
                     RGIRGLRVFSEPNGQHLSRLTGLIEAGALRPAQIESFPLEQAALAMDRSQAGHVRGKL
                     ALAVGQR"
     gene            complement(723..1121)
                     /locus_tag="AC16UZ_09145"
     CDS             complement(723..1121)
                     /locus_tag="AC16UZ_09145"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6246888.1"
                     /translation="MQYQSSLSRSGLETLAIESYFGNVDAKNLDAVLDCFHDNAMITV
                     QTAFTTHVGKDGIRRMFVDFMGAYKTIIHRDFTCTIDEKNGRIAACFTAELEDHDGNW
                     TTLQNTNFWRVRGDRFQEVYIYMSGENPLV"
     gene            complement(1149..1544)
                     /locus_tag="AC16UZ_09150"
     CDS             complement(1149..1544)
                     /locus_tag="AC16UZ_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6246889.1"
                     /translation="MADIPYEYYVDIVTKRYFHGVDKCDMDMVLDCFHEDAVLTEVTS
                     MTVHEGRDEGIRRMFEGLFDAHSRIWHGNFVHTADVDGEAICSQFSVEVTPKETGTEL
                     RYENCNRFYLDNGKFSRVFVYMSGENLLK"
     gene            complement(1578..>2173)
                     /locus_tag="AC16UZ_09155"
     CDS             complement(1578..>2173)
                     /locus_tag="AC16UZ_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009868041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR6246890.1"
                     /translation="YDTMLMDFSVFEAGLKAQEEGFAAICIDTVSDSGLYALRSRLDI
                     PVFGPGQAALHAACMLGHKFSIITMWDEWFHLYTKILKEYVLEQRCASLRSIKTRPDL
                     EELLAGKEEVIFKKLEEESLRAIEEDGADVIVLGSTTMHQSHAHLESVLPVPVINPGL
                     LCYKFCEVFLELGLSHSKRAFPSPEAPNDASVLGRHH"
BASE COUNT          378 a          708 c          672 g          415 t
ORIGIN      
        1 agcccgtggt ccacggcggc acgtatgccg agctcgtcac ggtgccggct ggcttcgtcg
       61 cgcccgcgcc cgcaaacctc gatgacgttg agacctccac catcccgctg gcgggcctga
      121 ccgtctggca atcgctcttc gagttcgcgg ggctgaccgc tggccagacc atcctgatcc
      181 acgccgcagc cggcggcgtg ggcagcctcg cggtgcagct ggccgcccat gcgggcgcgc
      241 gggttttcgc caccaccagc gcggcaaacg cagcctatgt ccgcgagctc ggcgcacacc
      301 ggatcatcga ctaccggcag gaaaatctgg gcgaggtcat ggggtccgaa gtgccagcgg
      361 gcttcgatgt ggtcttcgac accgttggcg gcgaggtcct gggcgagagc tacgacctgg
      421 tcaggcccgg cggcgccttg cccgcgctga acgacgcgcc cgacgaggcc ctatgcgccc
      481 agcgcggcat acgcgggctg cgcgtcttct cggagccgaa cggccagcac ctcagccgcc
      541 tgacggggct catcgaagcc ggcgccctgc ggccggctca gattgaatcg tttcccctcg
      601 agcaagcagc actcgccatg gatcgctctc aggccgggca cgtgcgcgga aagctcgcgc
      661 tcgccgtcgg gcaacggtag cttggctctt ccgccgtcga tgggctcccg ggcgccaggg
      721 gctcacacca gcggattctc cccgctcatg tagatgtaga cctcctggaa cctgtcgccg
      781 cggacacgcc agaagttggt gttctgcagg gtggtccagt tgccgtcgtg atcctccagc
      841 tccgccgtga agcaggcggc gatccggccg ttcttctcgt cgatggtgca ggtgaaatcc
      901 cgatggatga tggttttgta ggctcccatg aaatccacga acatccggcg gatcccatcc
      961 ttgccgacgt gggtggtgaa cgcggtctgc acggtgatca tggcgttgtc gtgaaaacaa
     1021 tcgagcacgg catccaggtt cttggcgtcc acgttgccga agtagctctc gatcgccagg
     1081 gtctcgagcc ccgaccggct cagcgaagac tgatattgca ctgggttatc ctcctttccc
     1141 gccccggctc acttgagcag gttctcgccg ctcatgtaaa cgaacactct ggagaacttg
     1201 ccgttgtcca gatagaagcg gttgcagttc tcgtagcgca gctccgttcc ggtctctttg
     1261 ggggtgacct ccacggagaa ctgggagcag atggcttcgc cgtcgacgtc cgccgtgtgg
     1321 acgaaattac cgtgccagat gcggctgtgg gcgtcgaaga ggccctcgaa catcctgcgg
     1381 ataccctcgt cccgcccttc gtggaccgtc atcgaggtca cttcggtgag caccgcgtcc
     1441 tcgtgaaagc agtccagcac catatccatg tcgcatttgt cgacaccatg aaagtatcgc
     1501 ttggtcacga tatcgacgta gtactcgtaa ggaatgtcgg ccatggggtt cacctccctt
     1561 ggttttaccg agctcgttca gtggtgccgg ccaagcaccg aggcgtcgtt gggcgcctcc
     1621 ggcgacggaa aggcccgctt gctgtggctg aggccgagct ccagaaagac ctcgcagaac
     1681 ttatagcaga gcaggccggg gttgatcacc ggcaccggca gcacgctttc gaggtgtgcg
     1741 tgagactgat gcatggtcgt ggatccaagc acgatcacgt cggcgccgtc ctcctcgatg
     1801 gcccgcaggc tctcctcctc cagcttcttg aagatcactt cctccttccc ggccagaagc
     1861 tcctccagat cgggacgggt ctttatcgaa cgcagcgagg cacacctctg ctcgagcacg
     1921 tactccttga ggatcttcgt gtagagatga aaccactcgt cccacatcgt gatgatcgag
     1981 aacttgtggc ccagcatgca ggccgcatga agcgccgcct ggccggggcc aaagaccgga
     2041 atgtcgagcc gcgagcgcag cgcataaagg ccggagtcgc tcaccgtatc gatgcagatg
     2101 gcggcgaagc cctcctcctg agccttcagg cccgcctcga acacgctgaa atccatgagc
     2161 atcgtgtcgt agt
//
LOCUS       DBNHOX010000411         6447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_740277, whole genome shotgun sequence.
ACCESSION   DBNHOX010000411 DBNHOX010000000
VERSION     DBNHOX010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_740277"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="AC16UZ_09160"
     CDS             complement(<1..1411)
                     /locus_tag="AC16UZ_09160"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017700408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR6246891.1"
                     /translation="MPFQKYRAFQAIDLPDRQWPTRTLTTAPRWCSVDLRDGNQALID
                     PMNVAEKLRMFDLLVGVGFAEIEVGFPAASQPDFDFIRRIIDEGRIPDGVAIQVLCQA
                     REELIARTVEALRGAPKVIFHLYNSTSELQRRVVFEMDRSGIIDLAVRGTRLVKAGLE
                     TLDSDITFEYSPESFTGTELDFAVDICTAVAEEWGATPDDPMIINLPSTVEMATPNIY
                     ADQIEWFVRNFSRRDSTVISLHTHNDRGTGIAATELALMAGAERVEGTLFGNGERTGN
                     CDIVTMAMNLFSQGVDPELDFRDMPRIRATVEEINKLPVHERHPYAGDLVFTAFSGSH
                     QDAIRKGMAKVDRDHWEVPYLPIDPADVGSSYKETVRVNSQSGKGGVGFLLEEHFGIA
                     LPREMLVEFSAVVQQLTESQGREIKTDEILEALKKEYVVDSGPYRLVDFDLLTGRTSD
                     QRCIARVEVNEQQVTIDGEG"
     gene            complement(1706..2893)
                     /gene="purT"
                     /locus_tag="AC16UZ_09165"
     CDS             complement(1706..2893)
                     /gene="purT"
                     /locus_tag="AC16UZ_09165"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010220253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HIR6246892.1"
                     /translation="MTSIGTPLAGNATRVMMLGAGELGKEVVIELQRLGVEVIAVDRY
                     ANAPGMQVAHRSHVISMLDGDALREVIALEKPDMVVPEIEAIATEVLVELESEGLTVI
                     PTARAARLTMDREGIRRLASETLGVRTSPYRFAATREEYDEAIQEIGVPCVVKPVMSS
                     SGKGQSTVRTAADAAAAWDYAQAGARGGADKVIVEGFVDFDYEITLLTVRHVGGTSFC
                     APIGHRQAGGDYQESWQPQPMAEAALAECKRIGELVTGELGGRGLFGMEFFIAGDQVY
                     FSEVSPRPHDTGMVTLLSQDLSEFALHARAILGLPIPHIQQHGPSASSVVLVEGESRA
                     VRFEGFEAALAEPGTDLRLFGKPEVAGERRMGVALARGRDVDDARKKANAAAAAIRWS
                     FDD"
     gene            complement(2890..3429)
                     /locus_tag="AC16UZ_09170"
     CDS             complement(2890..3429)
                     /locus_tag="AC16UZ_09170"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR6246893.1"
                     /translation="MLYQLGERRVCAEGAHFIAPGAMLTGSVTLKANANVWFNAVIRG
                     DVEPILVGANSNIQDCAVLHTDAGCPLTIGDYVTVGHKAVLHGCDVGDNTLIGINAVV
                     LSGARIGRNCIIGANALVTEGKVIPDNSMVLGSPGKVVRELVDEQIAGLRVSAEGYVE
                     RARWYLAELSEQESVDITS"
     gene            3604..3942
                     /locus_tag="AC16UZ_09175"
     CDS             3604..3942
                     /locus_tag="AC16UZ_09175"
                     /inference="COORDINATES: protein motif:HMM:NF022777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3135 domain-containing protein"
                     /protein_id="HIR6246894.1"
                     /translation="MKSRLPGFDTLVDMARNDPDRLESLRRSLTDAVIAEASCDDTRR
                     RLQGLQFRVDMERRRAATPLAATIRISEMMARSLADLHRSLVAPETLEEDLTRKPPRR
                     SDNVIEFPPS"
     gene            3994..4545
                     /locus_tag="AC16UZ_09180"
     CDS             3994..4545
                     /locus_tag="AC16UZ_09180"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003293638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6246895.1"
                     /translation="MKYCSACGFELVLRIPEGDNRERFLCEQCDTIHYQNPRIITGCL
                     AIWEEKVLLCRRSINPRRGFWTLPAGFLENGETAQAGAARETWEEARARVVIDDLYTM
                     FSLPHISQVYLFYKARLLDGSYAAGAETAEAGLFDEEEIPWDDLAFPVVRDTLQHYFQ
                     DRIHDHFRVHTGDIIVQRDRRSD"
     gene            complement(4556..>6447)
                     /gene="purL"
                     /locus_tag="AC16UZ_09185"
                     /gene_synonym="purI"
     CDS             complement(4556..>6447)
                     /gene="purL"
                     /locus_tag="AC16UZ_09185"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIR6246896.1"
                     /translation="DRSITGLVAREQMVGPWQVPCADVAVTLSGYRSYAGEAFAMGER
                     SPTALIDPAASARMAVGEALTNLCAAPVGDLGRVVLSANWMAAAGADAEEQALFEGVR
                     AVGMELCPALGIAIPVGKDSLSMRTRWDDREVTSPLTLIVSAFAPVTDARRTLTPELK
                     TTPDTRLLLVDLGQGRNRLGGSVLAQTYGQLGAEAPDVNSPDMLGAFFRAIQELNEQG
                     MLLAYHDRSDGGLIACVLEMAFAGRCGLDLRVDGEDVFGALFAEELGAVVQVAEKDLE
                     AVRAAFGEIPCAVIGCPRDDGVVVINGARAELYRSDRAALQEAWAETSYQIQRLRDNP
                     DCADEERSGIRAADPGFSSRVTFDVDEDVCAPYVGSSRPRIAVLREQGVNGQIEMAAA
                     FERAGFLPVDVHMSDVLEGRTRLLDFPALAACGGFSYGDVLGGGGGWAKSILFDDRAR
                     DQFVEFFARDRLALGVCNGCQMMSALKEIVPDAGSWPRFVRNRSEQFEGRTVLVRVNE
                     VDSPWLEGMAGSVLPIAVAHGEGRAEFADAGTELDFWESRRDQVCLQYVDNHHRPTEL
                     YPANPNGAGRGLAGLTGAFGRVLIMMPHPERVFRAYQNAWRDPSWQEDGPWMRLFRNA
                     RVALR"
BASE COUNT         1196 a         2201 c         1918 g         1132 t
ORIGIN      
        1 acccttcacc gtcgatggtc acctgctgct cgttgacctc tacccgcgca atacagcgct
       61 gatcagacgt ccggccggtg agcaggtcga agtcgaccag ccgataggga ccggaatcga
      121 ccacatactc tttcttcagg gcctcgagga tctcgtcggt cttgatctcc cggccctgac
      181 tctcggtcag ctgctgcacc acggcgctga actcgacgag catttcccgc ggtaaggcta
      241 tgccgaagtg ctcctcgagc agaaaaccca ccccgccctt gccggactgg ctgttcaccc
      301 ggaccgtttc cttgtaggag gaaccgacgt cggcgggatc gatgggcagg tagggcactt
      361 cccagtgatc cctgtccacc ttggccatgc ctttgcggat cgcatcctgg tgggagccgg
      421 agaaggcggt gaacaccaga tctcccgcat agggatggcg ctcgtgaacc ggcagcttgt
      481 tgatctcctc caccgtcgcc cggatacgcg gcatgtcacg gaagtcgagc tcggggtcca
      541 cgccctggct gaagagattc atcgccatgg tgacgatgtc gcagtttccc gtgcgctcgc
      601 cgttaccgaa cagcgtccct tcaacccgct cggcgcccgc catcagcgca agctccgtag
      661 cggcgatccc ggtgccgcga tcgttgtggg tatgaaggct gatcaccgtg ctgtcccgcc
      721 tcgagaaatt ccgtacgaac cactcgatct gatcggcgta gatgttgggt gtggccatct
      781 ccaccgtcga cggcaggttg atgatcatcg gatcgtccgg ggtggcgccc cactcctcgg
      841 ccacggcggt gcagatgtcc acggcaaagt cgagctccgt accggtaaag ctttccggcg
      901 agtactcgaa cgtgatatcg gaatccaggg tctcgaggcc cgccttgacc aggcgcgttc
      961 cccgcaccgc caggtcgatg atcccggagc ggtccatctc gaacaccacg cggcgctgca
     1021 attccgaagt cgagttgtat aggtgaaaga tcaccttcgg cgcgccacga agcgcctcca
     1081 cggttctggc aatcagctcc tcgcgagcct ggcagagcac ctgaatggcg acgccgtccg
     1141 ggatcctgcc ctcgtcgatg atgcggcgaa tgaaatcgaa atcgggctgc gaggcggccg
     1201 gaaaacccac ttcgatctca gcaaacccga caccgaccag caggtcgaac attcgcagtt
     1261 tctcggccac gttcatcggg tcgatcagcg cctgattgcc gtcgcgcaga tccacgctgc
     1321 accaccgcgg agccgtggtc agggtccggg tcggccattg ccggtccggc aggtcgatgg
     1381 cctgaaaggc acggtacttt tggaaaggca tggtgttcgc cgcgcgaccg ccggttggct
     1441 ggcgctgggc ccggaagggg gattgggttt gctcactcat tgttcttcgc ctgcgaggtc
     1501 tttctcgctg ctgctggtcc cggaggatcg ttaaggtttc gtatggggtg ggcggaatgc
     1561 ccttttgcgt aagtattggc cggctacgcc ggcagtcgca gcagcgccag aagagacaga
     1621 tttggacggt gatgagagtt caattcagct cccgaatcgt ggccgcgact atagccagcc
     1681 ggggaggatt tgccaaccct gctgatcagt cgtcaaacga ccaccgaatg gctgcggctg
     1741 ccgcgttggc cttcttgcgg gcatcgtcca cgtcccgccc ccgggccagc gcgacgccca
     1801 tgcgccgctc gcccgctacc tcgggctttc cgaacagacg caaatccgtg cccggctccg
     1861 cgagcgcggc ttcgaacccc tcgaacctga cggcgcgcga ttctccctcc accagcacga
     1921 cgctggatgc cgagggcccg tgctgctgga tgtgaggaat cggcaatccg agaatggccc
     1981 gggcgtgcag cgcaaactcc gagagatcct ggcttaggag cgtcaccatt ccggtgtcgt
     2041 gggggcgcgg gctcacctcg ctgaaataga cctggtcgcc tgcgatgaaa aactccatac
     2101 cgaagagacc acgacctccg agctcaccgg tgaccagctc gcctatgcgc ttgcattccg
     2161 ccaacgccgc ttcggccatg ggctgaggct gccaggactc ctgatagtcg ccgccggcct
     2221 gacgatgccc gatgggcgca cagaacgacg tgcctccgac atggcggacg gtgaggagcg
     2281 tgatctcgta atcgaaatcc acgaaaccct ccacgatcac cttgtcggca ccacccctgg
     2341 ccccggcctg cgcgtagtcc caggcagcgg cagcgtcggc cgccgtgcga accgtggact
     2401 ggcccttgcc cgaggagctc atcacgggct tgaccacaca gggaacgccg atctcctgta
     2461 tggcctcgtc gtactcttcg cgcgtcgccg caaatcggta gggcgaggtg cgcacgccca
     2521 gggtctcgga agccaggcgc cggatgccct cgcggtccat cgtgagccgg gctgccctgg
     2581 ccgtcgggat gaccgtcagg ccctcggatt ccaactccac caggacctcg gtggcgatgg
     2641 cttcgatctc ggggaccacc atgtcgggct tctccagcgc gatcacctca cgaagcgcgt
     2701 cgccgtcgag catgctgatc acgtggctgc gatgggcgac ctgcattccc ggcgcgttgg
     2761 cgtatcggtc gacggcaatg acctccacgc cgagcctctg cagctcgatg accacttcct
     2821 taccgagctc gccggcaccg agcatcatta ctcgcgttgc gttgcccgcc agcggcgtcc
     2881 ctatgctcgt catgacgtga tgtccacact ttcctgttcg gataactcgg caagatacca
     2941 ccgggcgcgc tcgacatatc cttcagcgct cactcgcagc cccgcaatct gctcatcaac
     3001 cagctccctg accaccttgc cgggcgaccc cagcaccatg ctgttgtcag gaatgacctt
     3061 gccctcggtt accagcgcat tcgccccgat gatgcagttg cgtccgatcc gggccccgct
     3121 cagcacgacg gcgttgatgc cgatgagggt gttgtcgcca acgtcgcaac cgtgcagaac
     3181 ggccttgtgc cccacggtga cgtagtcccc gatcgtcagc gggcagcccg catcggtgtg
     3241 caaaaccgcg cagtcctgga tgttgctgtt ggcaccgacc aagatcggtt ccacgtcacc
     3301 gcgaatgacc gcgttgaacc agacgttcgc gttggctttc aaggtcaccg atcctgtgag
     3361 catggcccct ggtgcaatga aatgcgcacc ctcggcgcat acacggcgtt caccgagctg
     3421 ataaagcact gctgcgtcac ccaaccccaa agcggcagag tgcagagtaa tgcaccaacc
     3481 gccggttcac aaagccctta cacaaattac gtggcgataa cgcccaccca caggcatagt
     3541 tttctgcggg gaacctccag cacgaccttg agccacaacg ataactagcg agtagggtcg
     3601 cttatgaaat cccggctgcc gggcttcgat acgctggtgg atatggctcg caacgatcca
     3661 gaccgactgg aatcgttgcg acgctcactg actgacgccg tcattgctga agccagctgc
     3721 gacgacacca gacgccgact acagggtctg cagttccgtg tggacatgga acggcgccgt
     3781 gcggcaacac ctctcgccgc gaccatccgc atctcggaga tgatggctcg ctcgctcgcc
     3841 gacctgcaca gatcgctggt ggcgccggaa acgctcgagg aagatctcac cagaaaaccg
     3901 ccccgacgga gcgacaacgt catcgagttt cctccgagct gagttgcccg aagccgcctg
     3961 cgacgggccg cgcagtcgcg ttatactcgc tgcatgaagt actgcagcgc ctgcgggttc
     4021 gagttggtct tacgcattcc cgagggagac aaccgggaaa ggttcctgtg cgagcagtgc
     4081 gacaccattc actaccagaa cccacggatc attacgggct gccttgccat ctgggaagag
     4141 aaggtcctgc tgtgcagacg ctcgatcaac ccgcgccggg gtttctggac gctgccggcc
     4201 ggttttctgg agaacggcga gaccgcccag gccggggccg cgcgtgaaac ctgggaggaa
     4261 gcgcgggccc gggtcgtaat cgacgacctc tacacgatgt tcagcctccc gcacatatcc
     4321 caggtctacc tcttctacaa ggcgcggctt ctcgatggat cctacgctgc cggggcggag
     4381 accgccgagg cgggcttgtt cgacgaggag gaaataccct gggacgacct ggcgttcccg
     4441 gtggtgcgcg acacccttca gcattacttc caggaccgca tccacgatca ctttcgggtt
     4501 cacaccggcg acatcatcgt tcagcgggat cggcgctcgg actgaaccga gcgccctagc
     4561 gcagcgccac ccgagcgttg cgaaacaagc gcatccacgg cccgtcttcc tgccacgacg
     4621 gatcccgcca ggcattctgg taggcgcgaa atacccgctc gggatgcggc atcatgatca
     4681 ataccctgcc aaaggcgccg gtcagccccg ccaggccgcg accggcacca ttgggattgg
     4741 ccggatacag ctcggtcggc cgatgatgat tgtccacata ctgcagacag acctgatccc
     4801 gccggctctc ccagaaatcc agctccgtcc cggcatcggc aaactcggcc cggccttcgc
     4861 catgggcgac cgcgatgggc agcacgcttc cggccatgcc ctcgagccat ggagagtcca
     4921 cctcgttgac gcgaaccaga accgtgcgac cttcgaactg ctctgagcgg ttgcggacga
     4981 agcgaggcca gcttcccgcg tccggaacga tctccttcag ggcagacatc atctggcaac
     5041 cgttgcagac acccagcgcc aagcggtcgc gcgcaaagaa ctcgacgaac tggtcgcgag
     5101 cccgatcgtc gaacaggatg ctcttcgccc aaccgccccc gccgccgagc acgtcgccat
     5161 aggaaaaccc tccgcatgcc gcgagagccg gaaagtcgag aagccgggtc ctcccttcca
     5221 gaacgtcgct catgtgcaca tccacaggca ggaaaccggc ccgctcgaaa gccgccgcca
     5281 tctcgatctg cccgttcacg ccctgctcgc gcagcacggc gatgcgcggg cgcgagctgc
     5341 ccacataggg agcgcagacg tcctcgtcga cgtcgaaggt cacgcggctg gagaatccag
     5401 gatccgctgc ccgaatgccg ctgcgttcct cgtcggcgca atcgggattg tcgcgcaggc
     5461 gctgtatctg gtagctggtt tccgcccagg cttcctggag cgccgcgcga tccgatcggt
     5521 acagctccgc ccgggcgccg ttgatgacga ccaccccgtc gtcgcgaggg catccgatga
     5581 ccgcgcacgg tatctcgccg aatgccgcgc gaaccgcttc cagatctttt tccgccacct
     5641 gaaccaccgc cccgagctct tcggcaaaca aggcgccaaa cacgtcttcg ccatcgacgc
     5701 gcagatccag cccgcagcgc ccggcgaagg ccatttccag cacgcaggca atgagaccgc
     5761 catccgaacg gtcgtgatag gccagcagca tgccctgctc gttcagttct tggatcgcac
     5821 ggaaaaaggc cccaagcata tcgggcgagt tcacatcggg agcttccgcc ccgagctgac
     5881 cataggtctg ggcaagcacg ctgccgccca gccggtttct gccctgaccg agatccacca
     5941 gcaggaggcg ggtatcgggg gtggtcttga gctcgggcgt gagggtacgg cgcgcatccg
     6001 tcaccggcgc aaacgcagag acgatgagcg tcagcggcga cgtcacttcc ctgtcgtccc
     6061 aacgagtgcg catggaaagg ctgtccttgc ccaccggtat ggcaattccg agggccggac
     6121 agagttccat ccccaccgcc cgtacgcctt cgaacagcgc ctgctcttct gcatcggccc
     6181 ccgcggcggc catccagttt gccgacagca ccaccctgcc aagatcgccg acaggcgccg
     6241 cacacaggtt ggtcaaggct tcgcccacgg ccattcgagc ggatgcggcg ggatcgatga
     6301 gcgcagtcgg tgaccgctcg cccatggcaa acgcctcgcc ggcatagctc cggtatccgg
     6361 atagcgtgac cgcaacatcc gcgcacggca cctgccacgg gcccaccatc tgctcccgcg
     6421 ccaccagccc ggtgatgctc cgatcgc
//
LOCUS       DBNHOX010000412         2969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_354926, whole genome shotgun sequence.
ACCESSION   DBNHOX010000412 DBNHOX010000000
VERSION     DBNHOX010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_354926"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..>2969
                     /locus_tag="AC16UZ_09190"
     CDS             117..>2969
                     /locus_tag="AC16UZ_09190"
                     /inference="COORDINATES: protein motif:HMM:NF016260.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HIR6246897.1"
                     /translation="MIAAVAVLMLSEPGSRFLVERATRLLPLEVDGVTGSLVGEMRFQ
                     RLRYELEGQIVEAQGLEVAFDPMAFLLKDEVRFRYVRAVRLRVETRGGEATSAGLGRF
                     ELDAPPITLVIDSLAVQVAEILDLPPSSMTLAGRWGARELSLAQVAVASEVLELRLSG
                     SLANGREPALDLVGSWSLPGTSWSGSGTLSGPLAALNVRHRLEGPVTLEAQGTVDLSS
                     LPDPRFELEVAMPEFSSEGFLLSDVLATVSGGRERVEGVVEGLVSTPWLTEPVPARLS
                     GSADLPPGEVRIAVDGFQAEVLGRPVEGRLVARQLAAGWRLEDVELDLSGSRVSGSAE
                     LDESGARASVHISAPDLASLDLGVAGDASGSVDFAAEAGSLSVSVSVSGSELSSGTAR
                     LLDYSVSGESRGREMNFVVRGDRAAQAGIEARMPSLQVDGPIDAPAWQLEWQGGAANG
                     IATREGEVLNLRVEALRFVALESSWRLSAPVKASADAAGVRVSAFCIEGPGSSGCVDE
                     LEMSGGKLVTAGRLVELPADLVSQWLPLPLVEGAVLEGQWRVVRDDGAWSGSAGLAAR
                     RLAYAVAGPGSVPIELPDVTVAAVFDDQVLRYTAEASDTDFELALDGQLSPIAANGSL
                     AGTLRLQADDVGPLRAFYQHVRELTGRLEGSVEFGGTLEEPRGAGSISLRDGSLSLER
                     PDLELRELQALLTLTEAGSVEVTGSASQADGRIELRGSGQDLFAAARAFDVELTGSDL
                     AVEHPDWTVRVSPDLRFTYTAGRGHLAGLIELPQAEIKVETLPAGAPRVSDDVVVVGR
                     DEQADTRRTRVTVDVTLVLGDQVALNAAGIAAQLEGRLNARVDAEGRQSLHGALQIEG
                     GAVLTQGQTLTIESGNVVYNGPVDNPYIDLRATRTIDTESPSVTVGVHIRGSADNLTS
                     TIFSDPAMSETRALSYLILGRDIGDPSDTDTSQLAAA"
BASE COUNT          422 a          929 c         1083 g          535 t
ORIGIN      
        1 gcccatcccc tcgacgatcc ggacaacgat ttccgcattc acatcagctt cgggccagat
       61 ctgtgaaacg tcttgttgca ggtattgtcg gcgttctcct ggcaaccgtt ttcctagtga
      121 ttgccgccgt cgccgtgctg atgctgagcg agccggggag ccggtttctg gtcgagcgcg
      181 caacccgcct gcttccgctc gaggtagacg gcgttacggg aagcctggtt ggcgagatgc
      241 gcttccagcg tctgcgctac gagctcgaag ggcagatcgt cgaggcccag ggactcgagg
      301 tcgccttcga tccgatggcc ttcctgctca aagacgaagt gcgtttccga tacgtgcggg
      361 ccgtgcgctt gcgcgttgaa acccgtgggg gagaagcgac atcggcaggc cttggccggt
      421 tcgagctcga cgcgccgccg attaccctgg tgattgactc gctggccgtg caggtggcgg
      481 aaattctgga tctgccgccg tcgagcatga cgctggccgg gcgctggggc gccagagagc
      541 tgagcctcgc tcaggtggcg gtggcctcgg aagtgctgga gctccgactt tccggatcac
      601 tggcaaacgg ccgcgaaccg gcgctcgacc tggtcggttc gtggtcgctg cccggcacat
      661 cctggagtgg ctctggcacg ctgagcggcc cgcttgccgc cctgaatgtt cgacatcgcc
      721 tggaaggccc ggtcacgctg gaagcgcagg gtacggtaga cctttcgagc ctcccggatc
      781 cccgtttcga gctcgaggtc gcgatgcccg aattttcgag tgaaggcttt ctgctgtcgg
      841 acgtcctggc caccgtgagc ggcggccgcg aacgggtgga aggcgtggtc gaggggctcg
      901 tctcgacacc gtggctcacg gagcccgtgc ctgctcgcct gtccggcagt gcggaccttc
      961 cgcccggcga ggttcgcatt gccgtggacg gtttccaggc cgaggtgcta ggcaggcccg
     1021 tggagggtcg gctggtggcg cggcagctgg cagcgggatg gcggctcgag gacgtggagc
     1081 tggacttgag cggcagccgt gtgtccgggt ctgcggagct cgacgagtcc ggcgcgcgtg
     1141 cctcggtgca catcagcgca ccggatctgg cctctctgga tctcggggtc gccggtgacg
     1201 cgtccggctc cgtcgatttt gccgcggagg cggggtcgct ctcggtgtcc gtgagtgtct
     1261 cgggaagcga gctctccagc ggtacggcca ggctgctgga ctactcggtg tctggggaga
     1321 gtcgcggccg cgagatgaac ttcgtcgttc gtggcgatcg cgcggcgcag gcggggatcg
     1381 aggcccgcat gccgagcctg caggtcgacg gcccgatcga cgcgccagcc tggcagctcg
     1441 agtggcaggg cggcgcagcc aacgggatcg ccacccgtga gggagaggtt ctgaatctgc
     1501 gcgttgaggc gttgcggttc gtcgctctgg agtcgagctg gcggctgagc gcgccggtta
     1561 aggcctcagc ggacgcggcc ggggtgcggg tgtctgcctt ctgcatcgaa gggccgggct
     1621 cgagtggctg cgtcgacgag ctcgagatga gcggcggcaa actcgtcacg gccggccgcc
     1681 tggtggagct gcctgcggat ctcgtgtctc aatggctgcc cctgccgctg gtggaaggcg
     1741 cggtcctgga gggccagtgg cgggtcgtcc gggatgatgg ggcctggtcg ggcagtgccg
     1801 gcctggcagc ccggcggctg gcgtatgcgg ttgccggtcc gggcagcgtg cccatcgagc
     1861 tgcccgacgt gacggtcgcg gcggtcttcg atgatcaggt cctgcgttac accgccgagg
     1921 cctcggatac ggacttcgag ctggccttgg atggtcagct gtcgcccatc gcggcaaacg
     1981 gctcgttggc gggcaccctg cgcctgcagg ccgacgacgt cgggcctttg cgggccttct
     2041 accagcatgt ccgcgagctc accgggcgac ttgaaggctc ggtagagttc ggcggaacgc
     2101 tggaagaacc tcggggtgca ggctcgatct ctttgcgcga tggaagtctc agcctggagc
     2161 gtccggacct cgagctcagg gaactgcaag cgttgttgac gctgaccgag gccggatccg
     2221 tcgaggtgac gggcagcgcc tcccaggccg atggccgaat cgagctgcga ggctcggggc
     2281 aggacctctt cgctgccgcc cgggcgttcg acgtcgagct gacggggtca gacctcgccg
     2341 tggagcatcc cgactggacg gtgcgggtgt cgcccgacct gcggttcacc tacacggccg
     2401 gccgcggaca tcttgcgggg ctcatcgagc tgccccaggc cgagatcaag gtagagaccc
     2461 ttcccgccgg cgcgccgcgt gtttccgacg acgtggtggt cgttggtcgg gacgagcagg
     2521 ctgacacgcg ccgcacccgc gtgacggtgg acgtgacgct cgtcctggga gaccaggtag
     2581 cgctcaacgc agcggggatc gccgctcagc tggaaggccg tctcaacgct cgggtggatg
     2641 ccgaggggcg gcaatcgttg cacggtgccc tgcagatcga gggcggggcc gtgcttaccc
     2701 agggtcagac gctgaccatc gagtccggca acgtcgtcta taacggcccg gtggacaatc
     2761 cctacatcga cctgcgcgct acccggacca tcgacaccga gagtccgtct gtcacggttg
     2821 gcgtgcacat ccgcggcagc gccgacaatc tgacttcaac gatattctcg gaccccgcga
     2881 tgtccgagac gcgggcgctt tcctatctga tcctgggccg cgacatcggc gacccgtcgg
     2941 atacggatac cagccagctt gcggccgca
//
LOCUS       DBNHOX010000413         6595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_226116, whole genome shotgun sequence.
ACCESSION   DBNHOX010000413 DBNHOX010000000
VERSION     DBNHOX010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_226116"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="AC16UZ_09195"
     CDS             <1..272
                     /locus_tag="AC16UZ_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246898.1"
                     /translation="APALDLWGIDGAERWSNGGLVRYRSRRDLMDQVVRTSRLDIHEF
                     KIAAMEKTIAYPLDPWWHLGDPRLLLALVLGCIGFGLQARGARGR"
     gene            complement(282..1163)
                     /locus_tag="AC16UZ_09200"
     CDS             complement(282..1163)
                     /locus_tag="AC16UZ_09200"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246899.1"
                     /translation="MARLAGKNIVVTGSSRGLGRAFAIAMAAEGASVVINGTNREALA
                     EVEKTISSAGGNVLAVPGSVTEETTCAQLVSGCVETFGSIDVMINNAGIVRDRTLLKM
                     TVEEWDDVLAVNLRGAFACTKHAALAMREAGGGHIVQVISASGLSGGFGQGNYAAAKA
                     GMMGLLRTAVLELSRFNIRNNALWPVAETDMTQVVFERAAAMSREQGAEPPTPAEMGF
                     GKPEEVAQGLVWLVSDGAAHFDGQCMSFNGRKTAIWRHPAEEHVSFHDEPWSFDELND
                     YYRDVKPLPIYRPVRAE"
     gene            complement(1249..2112)
                     /locus_tag="AC16UZ_09205"
     CDS             complement(1249..2112)
                     /locus_tag="AC16UZ_09205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6246900.1"
                     /translation="MKIGVFATFMSPLGTAEMIQDFGRRAEAAGLDSIWMGEHVVLFD
                     EMEFPYPGSRDGRIPVPEGGGLLDTVATFGLLAGSTSRLRFGTGICLVPQRNPIYTAK
                     EFATLDWLSGGRIDFGIGVGWCKEEVIACGYGWEDRGERCDEFLTLMRKLWSEPVVSY
                     SGKHVNVQGCRMDPKPIQNPHIPIIVGGHTPRAMRRAAEFGAGWYGFGITPDQTSAVL
                     TELDAALGAAGRSRDGFEIIITPASEDADSMAAFADLGVDRLILHLGSQRPEKLAARV
                     DQLASLVQAAA"
     gene            2212..3501
                     /locus_tag="AC16UZ_09210"
     CDS             2212..3501
                     /locus_tag="AC16UZ_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6246901.1"
                     /translation="MSDPASYNLMEDETRECPFPYFNAIRHEQPVYFMEELGAWYVSR
                     YEDVRHVKKHPEIFSNNIFEHGGHRGAERNIAEEYKSEHGWRRVSTLQRTDPPVHSKY
                     RKLINHAFTVSRIRKMTGYVETVVNDLIDQFIDKGEVDLVWEFAIPLPCTVIADQLGV
                     PRDKIWRLKEWSDAMLAPGGGFVDEAAALKCGQLVVEAQKFFAEVMEARRAEPRDDII
                     TDLVQAKVVDEPGGPERPLDMFELQDLLDQLLTGGNETTTNAIGSAMMLLLQRPGLME
                     KMRDDQKLIRNFLEESLRFETPVLHLWRITTQDTDLGGAFIPKGSMVAIGYASANRDE
                     DVFQDSEVFDIERQKAGAHLAFGSGPHHCPGAALARQEMFSAFTILLHRLDNIRLADP
                     GDRFLHVPSSFLRGLEHLNLKFDVRDRERYLPGASAY"
     gene            complement(3516..4337)
                     /locus_tag="AC16UZ_09215"
     CDS             complement(3516..4337)
                     /locus_tag="AC16UZ_09215"
                     /inference="COORDINATES: protein motif:HMM:NF006123.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6246902.1"
                     /translation="MSGDEQKSIFITGGAGGMGMATGRLFAQHGWFVGLFDLDESALE
                     LARAAFDDSQSLVRRLDVTSETDFADAMAAFSERTGGRMDVMFNNAGIAPGAWFDEMP
                     LDTIRQVIDINVMGVIIGIRAALPLLRATPGSLCMSTSSSVATYGHAMRAAYTASKFA
                     VKGLTEALSLEFERFGIRTADVLPGCIDTPMLRNALSARTGEPFDESLFETLPKEGAY
                     RLVPVSAIADAVWNAYHSDVIHWYVPEEVGEIDRLKGADFDAARAETRAFLFGEG"
     gene            complement(4374..5144)
                     /locus_tag="AC16UZ_09220"
     CDS             complement(4374..5144)
                     /locus_tag="AC16UZ_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6246903.1"
                     /translation="MQTILFESSGGVGTLTLNRPDRLNGMTNQMLIETHDCLLEVAQR
                     DDVRVLVLTGAGRGFCPGADLKHYAAGEADVASQPRHFQVPVLLHEMPQITIAAINGP
                     CAGAGLGWACGCDLRIAARSANFNTAFLKVASAGDMGGPWSLPRLIGAAKARELYFLA
                     EKFGAEEALAMGLVSRVFEDEQFAEKAAAMAGALASSSPHALRTMKQNFLDAERLNFA
                     DYVELETGRHLEVMAHPDAREAFRAFVEKRPPRFESSE"
     gene            complement(5160..5975)
                     /locus_tag="AC16UZ_09225"
     CDS             complement(5160..5975)
                     /locus_tag="AC16UZ_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6246904.1"
                     /translation="MSGYTDIDVQIEDPACLIRLNRPDKLNAFTYHTLREIRQAIDAA
                     AADSRVVGIIITGNGRGFSAGLDAQVLAEVTSRGGSAADDSAGEDDLPGIFSYLLEVP
                     KPVIAAVNGVAAGGGLILALMSDLRIASRDASFITVFLKRGLVAEHGSSWLLPRLVGT
                     GRALDLLWMSDRIDAQTAADIGLVERLAEPDELIPSALDYVTRLAEGSAPKAIAATKQ
                     LVYRHLGMGYADALKEADRVQRRFVAAADAAEGARALLEKRSPRFERLGTTDD"
     gene            complement(5972..>6595)
                     /locus_tag="AC16UZ_09230"
     CDS             complement(5972..>6595)
                     /locus_tag="AC16UZ_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246905.1"
                     /translation="GNQLLEVVAPIEEGTAGGRYLERRGGDGGYMVITQCDDHGPRRR
                     RVEALGIRIVNQYDSGEFRNMQMHPKDTGGSFFEIDEQLGGGAHDPDGPWEPAGPDWQ
                     QGRQLDRVSGIAAAEIQADDPARVAARWSEIAEIPLTTTADGHPALELDNATVRFVPC
                     TDGRPEGLGGIDVLATNAAAIMATARDQGLATGERQIYLCGMRINLV"
BASE COUNT         1056 a         2198 c         2200 g         1141 t
ORIGIN      
        1 cggcccccgc gttggatcta tggggaatcg acggcgcgga gcgctggagc aacggcgggc
       61 tcgtgcgcta tcgcagccgc cgggatctca tggatcaggt ggttcgaacc agtcgcctgg
      121 acatccacga attcaagatc gccgccatgg agaagaccat cgcctacccg ctggacccct
      181 ggtggcacct gggcgatccg cgtctgctgc tcgccctggt gctcgggtgc attggcttcg
      241 ggctgcaggc gcggggcgcc cggggccgct gagcgatccg cctactcggc gcgcaccggt
      301 cggtagatgg gtagcggttt gacgtcccgg tagtagtcgt tgagctcgtc gaagctccag
      361 ggctcgtcgt gaaacgacac gtgctcctcg gccgggtggc gccagatggc cgtcttgcgg
      421 ccgttgaacg acatgcactg gccgtcgaaa tgagcggccc cgtcgctgac cagccacacg
      481 agcccctggg cgacttcctc aggcttgccg aagcccatct ccgccggtgt ggggggctcg
      541 gcgccctgct cgcgggacat ggcggccgcg cgctcgaaca ccacctgggt catgtcggtc
      601 tccgccaccg gccacagcgc gttgttgcgg atgttgaagc gggagagctc cagcacggcc
      661 gtgcgcagca ggcccatcat gcccgccttg gccgcggcgt agttgccctg accgaacccg
      721 ccggagaggc ccgacgcgga gatgacctgg acgatgtggc caccgccggc ctcgcgcatg
      781 gcgagcgccg cgtgcttggt gcaggcaaac gcgccgcgaa ggttgacggc cagcacgtcg
      841 tcccactcct cgacggtcat cttcaggagc gtccggtcgc gcacgatgcc ggcgttgttg
      901 atcatgacgt cgatgctccc gaaggtttcc acgcagccgg acaccagctg tgcgcaggtg
      961 gtctcctcgg tgacggatcc ggggaccgcc agaacgttgc cgcccgcgga ggatatggtc
     1021 ttctcgacct cggccagggc ctcccgattg gtgccgttga tgaccacgct ggctccttcc
     1081 gcggccatgg cgatggcgaa agcgcgccct aagcccctcg acgagccggt gacgacgatg
     1141 tttttccctg caaggcgtgc catgagcaaa tgtctccagt ctggttgagg atccccccgc
     1201 ggccccattg tgccacgaga gcgctcacca ccggccggca cggccggttc aggcggccgc
     1261 ttggaccagt gacgccagct gatctacccg cgcggcgagc ttctccgggc gctgtgagcc
     1321 caggtgcaga atcaggcgat caacgcccag gtcggcaaag gcggccatgc tgtcggcgtc
     1381 ctccgacgcc ggggtgatga tgatctcgaa gccgtcccgt gaccggcccg ctgcccccag
     1441 cgccgcgtcc agctcggtca gcacggcaga tgtctgatcc ggggtgatcc cgaaaccata
     1501 ccagcccgcg ccgaactcgg cggcccggcg catggcgcga ggggtgtggc cgccgacgat
     1561 gatggggatg tgtgggttct ggatcggctt ggggtccatc cgacatccct gcacgttcac
     1621 atgcttgccg gagtagctga ccaccggctc gctccacagc ttgcgcatga gcgtgaggaa
     1681 ctcgtcgcat cgctcgcccc ggtcttccca gccgtacccg caggcaatta cctcctcctt
     1741 gcaccatccc accccgatgc caaagtcgat ccgtccgccg ctcagccagt ccagggtggc
     1801 gaactccttt gcggtgtaga tggggttgcg ctgggggacc aggcagatac cagtgccgaa
     1861 gcgcagccgg gaagtgcttc ccgccagcag cccgaaggtg gcgacggtgt cgagcagccc
     1921 gccgccctcg ggaaccggga tgcgaccgtc ccgggatccc gggtagggaa actccatctc
     1981 gtcgaataga accacgtgct cccccatcca gatggaatcg agacccgccg cctcggcccg
     2041 acggccgaag tcctggatca tctccgccgt gcccagcggc gacatgaagg ttgcgaacac
     2101 tccgatcttc atagcagttg cctccctgtt ctgttctaca gattctaggg gttcgccgcc
     2161 cgctttgctg gtaggattca gcggttgaat gtgtgtttgg tgggagagcg catgtcagac
     2221 ccggcgagtt acaacctgat ggaggacgag acgcgagagt gtccgtttcc ctatttcaac
     2281 gcgataaggc acgagcagcc cgtctacttc atggaagagc tcggcgcgtg gtacgtcagc
     2341 cgctacgaag acgtccgcca cgtgaagaag caccccgaga tcttttcgaa caacattttc
     2401 gagcacggcg gccaccgcgg cgcggagcgg aacatcgccg aagagtacaa aagcgagcat
     2461 ggctggcggc gcgtgtccac gctgcagcgg acggatccgc cggtgcattc gaagtatcgc
     2521 aagctgatca accacgcctt caccgtcagc cggatccgca agatgacggg atacgtggag
     2581 accgtggtca acgatctcat cgatcagttc atcgacaagg gggaggtgga tctggtctgg
     2641 gaattcgcca tcccgctgcc ctgcacggtg atcgccgacc agctcggcgt tccccgtgac
     2701 aagatctggc gcctcaagga atggtccgac gccatgctcg cacctggcgg tgggttcgtg
     2761 gacgaggcgg ctgcgctgaa gtgcgggcag ctggtggtcg aggcccagaa gttctttgcc
     2821 gaggtcatgg aggcgcgccg cgccgagcct cgcgacgaca tcatcaccga tctcgtgcag
     2881 gccaaggttg tcgacgagcc cggtggtccc gagcgtccgc tggacatgtt cgagctccag
     2941 gatctgctcg atcagctcct gacgggcggc aacgagacca ccaccaacgc catcgggtcc
     3001 gccatgatgc ttctgcttca gcggccgggg ctgatggaga agatgcggga cgaccagaag
     3061 cttatccgca acttcctcga agagtcgctg cgctttgaga cacccgtgct ccatctgtgg
     3121 cggatcacca cccaggatac cgatcttggc ggggcgttta ttcccaaagg atccatggtg
     3181 gccatcggtt acgcttcagc gaatcgtgac gaggacgttt tccaggattc cgaggtcttc
     3241 gacatcgagc gtcagaaagc gggcgcccat ctggcgttcg gctccggtcc gcaccactgc
     3301 cccggcgcgg ccctggccag gcaggagatg ttctcggcat tcaccatcct gctgcaccgg
     3361 ctggacaaca tccgcctggc ggatccgggc gaccgattcc tgcacgtccc cagcagcttc
     3421 ctgcggggcc tcgagcacct caacctgaaa ttcgacgtcc gcgaccggga gcgttatctg
     3481 ccaggggcgt ccgcttacta gcgacccccg cgcgctcagc cctcgccgaa caggaaagcc
     3541 cgggtctccg cccgggcggc gtcgaagtcg gcgcccttga ggcgatcaat ctcgcccacc
     3601 tcctcgggca cgtaccagtg gatcacgtcg gagtggtagg cgttccagac ggcgtccgcg
     3661 atggcggaaa ccggcacgag ccggtaggcg ccctccttgg ggagggtctc gaacagggac
     3721 tcgtcgaagg gctcgcccgt tcgcgccgac agggcattgc gcagcatggg cgtgtcgatg
     3781 cagcctggca gcacgtcggc ggtgcggatg ccgaagcgtt cgaactccag gctgagcgct
     3841 tcggtcaggc ccttcaccgc gaacttggag gccgtgtagg cggcgcgcat ggcgtgtccg
     3901 taagttgcca ccgaggagga cgtgctcatg cacagcgagc ccggcgtggc tcgcagcagg
     3961 ggcagggcgg cgcggatgcc gatgatcacc cccatgacgt tgatgtcgat gacctgacga
     4021 atggtatcca gcggcatctc gtcgaaccac gcccccggcg ctatccctgc gttgttgaac
     4081 atcacgtcca tgcgcccccc cgtgcgctcg gagaacgcgg ccatcgcatc ggcgaagtcg
     4141 gtctcgctgg tgacgtcgag ccgccggacg aggctctgcg agtcgtcgaa agccgcccgc
     4201 gcaagctcca gggcgctttc gtcgaggtcg aagaggccga cgaaccagcc atgctgggcg
     4261 aagaggcgtc cggttgccat gcccatgccg ccggccccgc cggtaatgaa aatcgatttc
     4321 tgctcgtccc cgctcacgtt gcgtctcctc gtaatggttt gtgcttggtc ggattactcg
     4381 ctggactcga aacgcggcgg gcgcttctcg acgaaagccc gaaacgcctc ccgggcgtcc
     4441 gggtgcgcca tgacctccag atggcggccg gtttcgagct ccacgtagtc ggcgaaattc
     4501 aggcgctcgg cgtcgagaaa gttctgcttc atggttcgca gcgcgtgggg ggacgagctc
     4561 gccagcgccc cggccatggc agctgctttc tcggcgaatt gctcgtcctc gaagacccgg
     4621 ctcaccaggc ccatcgccag agcctcctcg gccccgaact tctctgccag gaaatacagc
     4681 tcccgcgctt tcgcggcgcc gatgagacgg ggcagggacc agggcccgcc catgtcgccg
     4741 gcggacgcca ccttgaggaa cgccgtgttg aagttggcgg agcgggcggc gatgcgcagg
     4801 tcgcagccgc aggcccaacc gagaccggcg ccggcgcagg ggccattgat cgcggcgatg
     4861 gtgatctggg gcatctcgtg cagcagcacg ggaacctgga agtggcgtgg ctgggaagcc
     4921 acgtccgctt cgccggcagc gtagtgcttc aggtccgccc cggggcagaa accccggccc
     4981 gctccggtga gcaccagcac gcgcacgtcg tcccgttgcg ccacttcgag gaggcagtcg
     5041 tgggtctcga tcagcatctg gttggtcatg ccgttcagcc gatccgggcg gttcagggtc
     5101 agcgtgccca cgccgcccga tgattcgaag aggatggtct gcatgggctt cggctccgtt
     5161 cagtcgtctg tcgtgcccag gcgttcgaag cggggagagc gcttctctag cagggcgcgc
     5221 gcgccctcgg cggcgtctgc ggccgccacg aagcggcgct gcacccggtc ggcttccttc
     5281 agcgcgtcgg catagcccat gcccaggtgc cggtagacga gttgcttggt tgcggcaatg
     5341 gccttgggcg ccgagccctc cgccaggcgc gtgacgtaat cgagggcgct tggaatcagc
     5401 tcgtcgggtt ccgcgagcct ctccaccagg ccgatgtcgg ccgccgtctg cgcatcgatc
     5461 cggtcgctca tccagaggag gtccagcgcg cggccggtgc cgacgaggcg cggcaacagc
     5521 cagctcgacc cgtgctcggc cacgagcccg cgtttcagaa agacggtgat gaagcttgcg
     5581 tccctggaag cgatccgcag gtcgctcatc agcgccagaa tgagcccgcc gccggccgcc
     5641 acgccgttca cggcggcgat gacgggtttg ggcacttcca gcaggtagct gaaaattccc
     5701 ggcaggtcgt cttcacccgc ggaatcgtcg gccgcgctcc cgcctctcga ggtgacctct
     5761 gccagcacct gggcgtcgag gccggcggag aacccgcggc cgttgccggt aatgatgatg
     5821 ccgaccaccc gggaatcggc tgccgcggca tcaatggcct ggcggatctc ccggagcgtg
     5881 tggtaggtga aagcgttcag cttgtccggg cggttcagcc ggatcaggca ggcggggtcc
     5941 tcgatctgga catcgatgtc cgtgtagccg ctcatactag gttgatgcgc atgccgcaca
     6001 ggtagatctg ccgctcgccg gtggccaggc cctgatcgcg tgcggtggcc atgatggctg
     6061 cggcgttagt ggcgagcacg tcgatgccgc cgaggccctc cgggcgcccg tcggtgcagg
     6121 gcacgaagcg taccgtcgcg ttgtccagct ccagcgccgg gtggccgtcc gccgtggtgg
     6181 tgagcgggat ctccgctatc tcggaccagc gcgcggccac cctggcgggg tcgtctgcct
     6241 ggatctcggc tgcggcgatt ccgctcacgc ggtcgagctg ccggccctgc tgccaatcgg
     6301 ggcccgctgg ctcccagggg ccgtccgggt cgtgagcccc gccaccgagc tgctcgtcga
     6361 tctcgaagaa ggatcctccg gtgtccttgg ggtgcatctg catgttgcgg aactcgccgc
     6421 tgtcgtactg attgacgatg cggatgccga gcgcctctac ccggcgccga cgaggcccgt
     6481 gatcgtcgca ctgggtgatg accatgtacc cgccgtcacc gccgcgccgc tccaggtagc
     6541 ggccgcctgc cgtgccttct tcaatgggcg ccacgacctc cagcagctga ttgcc
//
LOCUS       DBNHOX010000414         3321 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_290896, whole genome shotgun sequence.
ACCESSION   DBNHOX010000414 DBNHOX010000000
VERSION     DBNHOX010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_290896"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="AC16UZ_09235"
     CDS             complement(<1..691)
                     /locus_tag="AC16UZ_09235"
                     /inference="COORDINATES: protein motif:HMM:NF018649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR6246906.1"
                     /translation="MAQPTQTARGGPGRGSAFGWIWIAYLAAVVVGVAGTMIVADPAT
                     DHPVYLAGIADVLATVVIFAFSVAFRNSSFYDPYWSVVPPVLGLYWWWVVGSVDDPRV
                     WLAMLVVSCWAVRLTYNWAFGWTGLGHEDWRYRDLQNRLGILYWPVSFLGIHMMPTVL
                     VFLGCVPLFLIMGRESGPLNGIDLLALAVGAAAVWIETRADLELHRFRARRGSPAEAL
                     TTGVWGWCRHPN"
     gene            complement(695..2461)
                     /locus_tag="AC16UZ_09240"
     CDS             complement(695..2461)
                     /locus_tag="AC16UZ_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012271558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6246907.1"
                     /translation="MRGIVLFLGAATLLLAGCDGSAPDAPGVDRHAAAKRWITQEFQP
                     STLTPDEQAAEMAWFIEAAAPFRGMEISVVSETLTTHEYEAGVLARAFAEITGIRVSH
                     DLIQEGDVIEKLQTQMQSGRNVYDAYVNDSDLIGTHARYGYVVALSDFMAGEGADVTL
                     PTLDVDDFIGRSFTTGPDGKLYQLPDQQFANLYWFRYDWFQREDLRARFRELYGYDLG
                     VPVNWSAYEDIAEFFSEYVRTIDGQRIYGHMDYGKKDPSLGWRFTDAWLSMAGAGDPG
                     IPNGLPVDEWGIRVDGCRPVGASVSRGGAANGPAAVYALEKYIDWLKRFAPPQAPGMT
                     FSEAGPVPAQGHIAQQIFWYTTFTADMIDGDLAVVDEDGLPRWRMAPSPHGPYWEEGM
                     KLGYQDAGAWTLLASTPLERRKAAWLYAQFVVSKTVSLKKTLVGLTPIRDSDLRSAAM
                     TEAAPRLGGLVEFYRSPARTLWTPTGTNVPDYPKLAQLWWPNVANAVSGAVSAQEAMD
                     TLARDQDRVLERLQRSGIQGDCGPELNPEVDPEEWLARPGAPKPRLADEKPPGKTVPY
                     DELVADWGALPRPGEPLGRRSH"
     gene            complement(2527..2805)
                     /locus_tag="AC16UZ_09245"
     CDS             complement(2527..2805)
                     /locus_tag="AC16UZ_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007946969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2160 domain-containing protein"
                     /protein_id="HIR6246908.1"
                     /translation="MSFSWMAWTLPTAIFFGAILCLLVVMTVLEIRWPTRTRRGLLPL
                     ATTRGDRLFIGLLVAAFVHILWLAALDAPVLLATGISLGLLALLIRWG"
     gene            complement(2825..>3321)
                     /locus_tag="AC16UZ_09250"
     CDS             complement(2825..>3321)
                     /locus_tag="AC16UZ_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018925489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6246909.1"
                     /translation="LLTNRMAPPAVFLLPFFQLYSAVGLFDTPLAVALAHCLFTVPLA
                     VWILEGFMSAVPRSLDEMARIDGHSLPYFFFRIFLPVIRGGVGVTVFFCFMFSWVELL
                     LARTLTASAAKPIVVVMTRTVSASGMDWGVLAAAGVLTIVPGVAVVWFVRRHLVKGFA
                     LGRV"
BASE COUNT          649 a         1129 c         1054 g          489 t
ORIGIN      
        1 agttgggatg gcggcaccaa ccccagaccc cggttgtgag ggcctcggca ggtgagccgc
       61 gacgggcccg aaagcgatgc agctcgagat cggcgcgggt ttcgatccag accgccgcgg
      121 cccccaccgc cagggccagc aggtcgatgc cgttcagggg ccccgattcc cgacccatga
      181 tcaggaaaag cggcacgcac ccgaggaaga ccagcaccgt gggcatcatg tgtatcccaa
      241 ggaagctcac gggccagtag aggattccca gcctgttctg caggtcgcga taccgccagt
      301 cttcgtggcc aaggcccgtc cagccgaaag cccagttgta ggtcaggcgt accgcccagc
      361 agctgacgac gagcatggca agccagaccc gcggatcgtc tacggagccc acaacccacc
      421 accagtaaag gcccagcacc ggtggcacca cgctccagta cggatcgtag aaactggaat
      481 ttcggaaagc gacgctgaat gcgaagatca cgacggtggc cagaacgtcg gcaatgccgg
      541 ccagataaac ggggtgatcg gtggccgggt cggcaacgat catcgtgccg gccacaccca
      601 ccacgacagc tgccagatag gcgatccaga tccagccaaa cgcgctgccc cggcccgggc
      661 cgcccctggc agtctgtgtt ggttgtgcca tgtttcagtg actccttcgg ccgagcggct
      721 cgcccggccg cggcaaagcc ccccagtctg ccaccagctc atcgtaggga accgtcttgc
      781 cgggcggctt ctcgtcggcc aggcggggct tgggcgcacc gggccgtgcg agccactctt
      841 cgggatccac ctccggattc agctccggtc cgcaatcgcc ctggatgccc gaccgctgaa
      901 ggcgctccag cactctgtcc tgatcccgcg ccagggtatc catggcctcc tgagcgctga
      961 cggcgccgct caccgcattg gccacgttgg gccaccacag ctgtgccagc ttcggatagt
     1021 cgggcacgtt cgtccccgtg ggcgtccaga gcgtccgcgc cgggctcctg tagaactcca
     1081 ccagcccgcc cagacgcggc gcggcctcgg tcatggccgc cgaccgcagg tcggaatcac
     1141 gaatgggcgt cagccccacc agggttttct tgagggagac ggtcttggaa accacgaact
     1201 gcgcgtacag ccaggcagcc ttcctgcgct ccagcggcgt gctggcaagc agcgtccagg
     1261 cgccggcatc ctgataaccg agcttcatgc cttcctccca gtaaggtcca tggggggacg
     1321 gcgccattcg ccatcgcggc aggccgtctt cgtcgaccac ggccaggtcg ccgtcgatca
     1381 tgtccgcggt gaaggtcgtg taccagaaaa tctgctgggc gatgtgaccc tgggccggca
     1441 ccggcccggc ttcggagaaa gtcatccccg gggcctgcgg gggcgcgaag cgcttcagcc
     1501 aatcgatgta cttctccagc gcgtagacgg ccgcgggccc gttggccgcc ccgccgcgac
     1561 tgacggaggc gccgaccgga cggcagccgt ccacccggat tccccattcg tccacgggca
     1621 ggccgttcgg gatccccggg tctccggcac cggccatgga cagccaggca tcggtgaagc
     1681 gccagcccag cgacgggtcc ttcttcccgt aatccatgtg gccgtagatc cgctggccgt
     1741 cgatagtccg gacgtattcg gagaagaact ccgcgatgtc ctcgtatgcc gaccagttca
     1801 cgggcacgcc gaggtcgtag ccgtagagct cgcggaatcg ggcacgaaga tcctcgcgct
     1861 ggaaccagtc gtatcgaaac cagtagaggt tggcgaactg ctggtccggc agctggtaga
     1921 gcttgccatc cggacccgtg gtaaagctcc ggccgatgaa atcgtccacg tccagggtgg
     1981 gaagggtgac gtcggcgccc tcgccggcca tgaagtcgga cagcgcgacc acgtaaccgt
     2041 aacgcgcgtg ggtgccgatg aggtcggagt cgttgacgta ggcgtcgtag acgttgcggc
     2101 cggactgcat ctgggtctgc agcttctcga tgacgtcgcc ttcctgaatg agatcgtggg
     2161 acacccggat acccgtgatc tcggcgaagg cccgagcgag cacccccgct tcatactcgt
     2221 gagtggtcag ggtttccgag acgacgctga tttccatgcc cctgaacggc gcggcagcct
     2281 caatgaacca cgccatttcc gccgcctgct cgtctggcgt gagcgtggag ggttgaaact
     2341 cctgggttat ccagcgcttc gccgccgcgt gccggtcaac cccgggcgcg tccggcgcgg
     2401 acccatcgca accggcgagc agcaaagtcg ccgccccaag aaaaagaacg atcccccgca
     2461 tccgcatccc ccctggccga acggcgcata ccttagcagg aaacgccagg aaccgtgctc
     2521 gaccaatcag ccccagcgga tgagcagcgc gagaagcccc aggctgatgc cggtcgccaa
     2581 gagcacgggc gcgtcgagcg cggccagcca gaggatgtga acgaaggccg ccaccagcaa
     2641 accgatgaac agacgatcac cgcgcgtggt tgccagcggc agaagacccc ggcgcgtcct
     2701 cgtcggccat cgaatctcga gcaccgtcat gaccacgagc aggcagagaa tcgcgccaaa
     2761 gaagatcgcg gtggggagcg tccacgccat ccaggaaaaa ctcattgccc tgcggccccc
     2821 gctttcacac ccgccccagg gcgaagccct tcacgagatg ccttctgaca aaccacacca
     2881 cggcaacgcc cgggacgatg gtcagcacgc cagcggccgc cagcacgccc cagtccatgc
     2941 cggaagcgct gaccgttcgg gtcatgacca cgacgatggg tttcgccgcg ctcgctgtga
     3001 gcgttcttgc cagcagcagc tcgacccagc tgaacatgaa gcagaagaac accgtcacgc
     3061 caacgccgcc tcggatgacc ggcagaaaaa ttctgaagaa gaagtagggg aggctgtggc
     3121 catcgatgcg cgccatctcg tccaggctgc gggggaccgc cgacatgaaa ccttccagaa
     3181 tccacaccgc cagaggcacc gtgaagaggc agtgggcgag cgccactgcc agcggcgtgt
     3241 cgaagagccc gacggcggaa tagagctgaa agaacggcag cagaaagacg gcaggcggag
     3301 ccatccggtt ggtgagcagc c
//
LOCUS       DBNHOX010000415         3052 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1319170, whole genome shotgun sequence.
ACCESSION   DBNHOX010000415 DBNHOX010000000
VERSION     DBNHOX010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1319170"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="AC16UZ_09255"
     CDS             complement(<1..209)
                     /locus_tag="AC16UZ_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246910.1"
                     /translation="MKIFSRIKGPRLGTKIMLLGLTLLIVPWFSYRQLVEMERLLIQG
                     QSQAQLLTAEGISTLFNGREDLFNDL"
     gene            complement(225..947)
                     /locus_tag="AC16UZ_09260"
     CDS             complement(225..947)
                     /locus_tag="AC16UZ_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6246911.1"
                     /translation="MPPRRHTGRAKTRAIVEDDPDQRANYADAITMKGYKVSAYGSRQ
                     EALEGFDQALPDLVILDIILGEEVDAGFQLCRDLLARAPSLPVIFLTERINEIDKISG
                     LRLGAWDYLPKPISLDYLAERISSLLRINEVRAEQQETESATAKRIGDLMLDQDALLV
                     SWKGHRIDLSGTEFRMLAKLVRMPGHAVSYETLMNATMQSLVTNNTINTHMRNIRKKF
                     EKVDKDFSSIKSEYGFGYRWSA"
     gene            1139..1321
                     /locus_tag="AC16UZ_09265"
     CDS             1139..1321
                     /locus_tag="AC16UZ_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246912.1"
                     /translation="MKVFIPFSEALVEESGFPAGELVPFQLEYECWRHLGENADCESA
                     GAASGQGTAQTVELPA"
     gene            1368..1976
                     /gene="pnuC"
                     /locus_tag="AC16UZ_09270"
     CDS             1368..1976
                     /gene="pnuC"
                     /locus_tag="AC16UZ_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HIR6246913.1"
                     /translation="MPEIFEQIIAMAWLESVGLGSGLLCVWLLIRQNIWTFPIGLVYS
                     VVSVGVFAHERLYADVLLSVYYVGMNAYGWYYWLYGGRRTVADLLPVTHTPRPTLLWL
                     AGVTALATFVMGWFFDTQTNASLPYWDSATTCMSFAAMWMTARKYIENWVVWLVVDVM
                     ATVIYLVKGIELYAVLYGIYLAMAIMGWRAWRRSLNLASSSA"
     gene            1937..2482
                     /locus_tag="AC16UZ_09275"
     CDS             1937..2482
                     /locus_tag="AC16UZ_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6246914.1"
                     /translation="MATEFKPGLVVCLTGPESTGKSTIAEQLASALAVPLVPEVAREY
                     LAGRPDYEQSDLLAIARAQLAAEQDALAEGTGLVVCDTDLQVIQIWWEERYAELDPWL
                     AQALVDRSPRRYLLAVPDLPWEPDPLRESPHDRERLFRRYRQVLREGAFPYAEVRGLG
                     RSRFDSALVTVQRWLAEPQQD"
     gene            complement(2484..>3052)
                     /locus_tag="AC16UZ_09280"
     CDS             complement(2484..>3052)
                     /locus_tag="AC16UZ_09280"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6246915.1"
                     /translation="WLGSRWVEGPLLVDAPPSAQTIVGFIRRLHESLRVRAAPYDPAA
                     RSRAYLETPGDSSPDARVMAIAHSLNWRLDDTVACHNDLNPWNVIVSASGDWVTLDWQ
                     SVGANDPLFDLVTLHQGLSLPDAGLTDLCEALLERPVAPERVERVLVGFWLREYAWAF
                     AALQLGNRRQEILDQLALSRDRLASLAG"
BASE COUNT          512 a          953 c          989 g          598 t
ORIGIN      
        1 agatcgttga acagatcctc ccggccgttg aagagggtgg agatgccttc ggcggtgagc
       61 agctgggcct gtgactggcc ctgaatgagc agccgctcca tctccaccag ctggcggtag
      121 ctgaaccagg gaacgatgag cagggtgagc ccgagcagca tgatcttggt gccgagccgg
      181 ggacccttga tgcgactgaa gatcttcatg ggcgttggcg agcgtcaggc cgaccagcga
      241 tatccgaacc cgtactcgct cttgatgctc gagaagtcct tgtcgacctt ttcgaacttc
      301 ttgcggatgt tgcgcatgtg ggtgttgatg gtgttgttgg tgacgaggct ctgcatggtc
      361 gcgttcatga gcgtttcgta gctcacggca tggcccggca tgcgaacgag ctttgccagc
      421 atccggaact cggtgccgga cagatcgatg cggtgacctt tccaggacac cagcagcgcg
      481 tcctggtcga gcatgaggtc gccgatccgc tttgccgtgg cgctttccgt ctcctgctgc
      541 tcggcccgca cctcgttgat gcgcagcagc gaggagatcc gctcggccag gtagtccagg
      601 ctgatgggct tgggcaggta gtcccaggcg cccagacgaa gccccgagat cttgtcgatc
      661 tcgttgatcc gctcggtgag gaagatgacc ggcaacgatg gcgcgcgggc gagcaggtcc
      721 cggcaaagct gaaagccagc gtccacctcc tcgccgagaa tgatgtccaa gatgaccaga
      781 tcgggcagcg cctgatcgaa gccctccagt gcttcctgcc ggctcccgta ggcggaaacc
      841 ttgtagccct tcatggtgat cgcgtcggcg tagttggccc gctgatcggg gtcgtcctcg
      901 acgatcgctc tggttttcgc cctacctgtg tgcctgcgcg gcggcaaaat ccgggtggat
      961 tctagcccgt tttggaagtt ttagcgcgag gagtgatcca tgcacggtca ctggtccaca
     1021 gtttctccat gatcatcaat cacgactcca cgttttctgg cggaagcgtg ggttaagggc
     1081 aggtttaatg ctttagacct gcgaagagca gcacagcaag gatgttttgc gcttagtcat
     1141 gaaagtcttc attcccttca gcgaggcgct cgtcgaggaa tccgggtttc ccgccggcga
     1201 gctggtgccc ttccagctcg agtacgagtg ctggcggcat ctgggcgaga acgcggactg
     1261 cgaatccgcg ggggccgcgt ccggtcaggg caccgcccag accgttgaat tgccagccta
     1321 gaacctagtc tctatcgttg ggcgctcctt taccgagcgt ccatccgttg ccggaaatct
     1381 ttgagcagat catcgccatg gcgtggctcg aatccgtagg tctgggttcg gggctgttgt
     1441 gcgtgtggct tctcatccgt cagaacatct ggacctttcc catcgggctc gtctactccg
     1501 tcgtctccgt cggcgttttt gctcacgagc ggctctacgc cgacgtgctg ctcagcgtct
     1561 actacgtcgg gatgaacgcc tatggctggt actactggct ttacggcggc aggcgcaccg
     1621 tggccgatct gcttccggtg acccacacgc cgcggcccac gctgctgtgg ctcgccgggg
     1681 tgaccgcgct cgccaccttc gtcatgggct ggttcttcga cacccagacc aacgccagcc
     1741 tcccctactg ggacagcgcc accacctgca tgagcttcgc cgccatgtgg atgacggccc
     1801 ggaaatacat cgagaactgg gtcgtctggc tcgtcgtgga cgtcatggcc acggtcatct
     1861 atctggtcaa gggcattgag ctgtatgctg ttctctatgg catttatctg gcaatggcca
     1921 tcatgggctg gcgcgcgtgg cgacggagtt taaacctggc ctcgtcgtct gcctgacggg
     1981 ccccgagagc accggcaaga gcaccatcgc ggagcaattg gccagcgccc tggccgtccc
     2041 gctggtgccg gaggtcgcgc gggagtatct cgccggaaga cccgactacg aacagtccga
     2101 cctgctggcc atcgcccggg cgcagctcgc cgcggagcag gacgccctgg ccgaaggcac
     2161 cggtctcgtc gtgtgcgata ccgacctgca ggtcatccag atctggtggg aggaacgcta
     2221 cgcggagctg gacccgtggc tggcccaggc gctcgtcgac cgttcgcccc gtcgctacct
     2281 gctcgccgtt ccggatctac cctgggagcc cgaccccctg cgcgagtcgc cccacgaccg
     2341 ggagcggctc tttcgacgct accggcaagt cctgagggaa ggcgcttttc cgtacgccga
     2401 agtccgcggt ctggggcggt cgcggttcga ttccgcgctg gttaccgtgc agcgctggct
     2461 ggccgaaccc cagcaggatt gaatcagccg gccagagaag ccagccggtc acgggacagg
     2521 gcgagctgat cgagaatttc ctgacgccgg ttaccaagct gaagggcggc aaaggcccag
     2581 gcgtactcgc gcagccagaa tcccacgagc actcgctcca cccgctcggg cgcgacgggc
     2641 cgctccagca gcgcttcgca caggtccgtc agcccggcgt ccggcaggct caaaccctga
     2701 tgcagggtca ccagatcgaa cagcgggtcg ttggcgccga cggactgcca gtcgagggtc
     2761 acccagtcac cgctggccga gacgatgacg ttccacgggt tgagatcgtt gtggcaggcg
     2821 acggtgtcgt ccaggcgcca attaaggctg tgggcgatgg ccatcacccg ggcgtccggt
     2881 gacgagtctc cgggcgtttc gagataggca cggctgcgcg ccgcaggatc gtaaggcgct
     2941 gccctcacgc ggagcgattc gtgcagccgc ctgatgaaac cgacgatggt ctgcgctgac
     3001 ggcggggcgt cgacgagtaa ggggccttcg acccagcggg atcccagcca gc
//
LOCUS       DBNHOX010000416         3217 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_933602, whole genome shotgun sequence.
ACCESSION   DBNHOX010000416 DBNHOX010000000
VERSION     DBNHOX010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_933602"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2797)
                     /locus_tag="AC16UZ_09285"
     CDS             complement(<1..2797)
                     /locus_tag="AC16UZ_09285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HIR6246916.1"
                     /translation="MGIYHHNPLTVGTLWALALLWAAVPLLMARRANRSTLDEAARNA
                     LESSPCGSAVIDATGRIVWVNAAFAAQIDRGSDSMAGTLLSDVAREDLWAELESRREE
                     LDRGVVIDVSSCLVRADGEEVWVAGHVRAMEPESGRLCLQLVDVSSALGAQDSLVKSV
                     AEFRQALESSNDLILMVSKHGKINYANPVATSCLAGDADLNEAVITDLVELNDRERFI
                     TALKNSRKKGEVAELSGLHLLRPDRDSDFPVAARIAGLRTPDGVGGAIVTCRLMDDQI
                     ASERELRSSEARFSRIFHSSPDAILIVRNADSTILDFNAGFIRLLGYTREDAIGQAES
                     DMQLWVHEAERERILNNLQTDQEVTAQETKLRRKNGAVVYVELSLRYIEIEGELCILW
                     IGRDVSERAIAEIARRQSEEKFVEVFNRSPDGIAIVRQEDMLICDINEAFVNSSGHQR
                     EDLIGESLFELDVVSESALADAALRLEEDDHFSNLEMTLHTKSGEEIPALVSATTLEV
                     EEEPCFLIIAKDLRDLRATEEQLRASEQRFRGAFENAPIGILLVDMDGKVFQANRFAA
                     ELLSYRNASMRNLHISRLVPDDDRDKYNDLMQRLARGHQATLRTEQRMLCADGAEIWT
                     NMHVVLLRSQDGEPAYGIVQIADITEMKHSQQRMERMAFYDILTDLANRRLFYDRLSQ
                     AIEYTTRNNRLAALLYLDLDQFKRVNDTLGHEAGDTLLRTVAERLSHNVRQEDTVARP
                     GGDEFTVLLYNIGKPEDAGHVAEKILNELRRPITVAGEQLVMTTSIGISIIPSDGTDP
                     KQLMSNADLAMYRAKEQGRNQFQFYSKEMNTNAAHRLKTENELRAALERQEFELYYQP
                     KVRLSDLQIVGVECLLRWHHPERGLLLPGEFIEVAEETGTIVDLGKWVIETACSAATQ
                     RAHEAGEPICTAVNISPR"
     gene            2942..>3217
                     /locus_tag="AC16UZ_09290"
     CDS             2942..>3217
                     /locus_tag="AC16UZ_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR6246917.1"
                     /translation="MNYTLSVSTGRQGLYDITHKVVECLGRSGISEGLVTVFVRHTSA
                     SLLIQENADPSAREDLESWLNRLVPEVDELYTHRAEGPDDMPAHIKSA"
BASE COUNT          521 a         1004 c         1066 g          626 t
ORIGIN      
        1 ggcgcggcga gatgttcacc gccgtgcaga tcggctcgcc tgcctcatga gcccgctggg
       61 tggcggcgga gcaggccgtc tcgatgaccc acttgccgag atcgacgatc gtgccggttt
      121 cctcggcgac ctcgatgaac tctcccggca gcagcaggcc gcgctcgggg tgatgccagc
      181 gcagcaggca ctccacgccc acgatctgca gatcggacag gcgcaccttg ggctggtagt
      241 agagctcgaa ctcctgccgc tcgagagcgg cccgcagctc gttctcggtc ttcagacggt
      301 gggcggcgtt ggtgttcatc tccttgctgt agaactggaa ctgattgcgc ccctgttcct
      361 ttgcccggta catcgcgagg tcggcgttgg acatgagctg cttgggatcc gtgccgtcgc
      421 tggggatgat cgatatgccg atgctggtgg tcatcaccag ctgctccccg gccaccgtga
      481 tcggtcgtct cagctcgttg agaatcttct cggcgacgtg ccccgcgtct tccggcttgc
      541 cgatgttgta gagcagaacg gtgaactcat cgcccccggg cctggcgacc gtgtcctcct
      601 ggcgcacgtt gtggctcagc cgctcggcca ccgttcgcag gagggtgtct cccgcctcgt
      661 ggccgagggt gtcgttcacc cgcttgaact ggtcgaggtc caggtacagc agagcggcaa
      721 ggcggttgtt gcgcgttgtg tactcgatgg cctgggagag ccggtcatag aacagacgcc
      781 ggttggcgag gtcggtgagg atgtcgtaga aggccatgcg ctccatgcgc tgctggctgt
      841 gcttcatctc cgttatgtcg gcaatctgca cgatgccgta agccggctcc ccgtcctgag
      901 atcgcaggag caccacgtgc atgttggtcc agatctctgc gccgtccgca caaagcatgc
      961 gctgctcggt gcgcagcgtg gcctgatgcc ctctggcgag ccgctgcatc aggtcgttgt
     1021 acttgtcgcg atcatcatcg ggaaccagac gcgagatgtg caggttcctc atggaggcgt
     1081 tgcgatacga caggagctcc gccgcgaacc ggttcgcctg aaagaccttg ccgtccatgt
     1141 cgaccagcag aatgccgatg ggcgcgttct cgaaagcacc gcggaagcgc tgctcactgg
     1201 ccctcagctg ttcctcggta gcgcgcagat cgcgaaggtc cttggcaatg atgaggaagc
     1261 agggttcctc ttcgacctcg agcgtggtgg ccgacaccag ggcgggaatc tcctcgccgg
     1321 acttggtgtg aagggtcatc tccaggttgg agaagtggtc gtcctcctcc aggcgcaggg
     1381 cggcatctgc gagggccgac tcgctcacca cgtccagctc gaaaagggac tccccgatca
     1441 gatcctcgcg ctggtggcct gaggaattca cgaacgcttc gttgatgtcg cagatcagca
     1501 tgtcttcctg gcggacgatg gcgatgccgt ccgggctgcg gttgaagacc tcgacgaatt
     1561 tctcctcgct ctgccgtcgg gcgatctcgg cgatggcccg ctcggacacg tcccgaccga
     1621 tccacaggat gcacagctcc ccctcgatct cgatgtagcg cagggacagc tccacgtaca
     1681 ccaccgctcc gttcttgcgg cgcagcttcg tctcctgggc ggttacttcc tggtcggtct
     1741 ggaggttgtt gagaatgcgt tcccgctccg cctcgtgcac ccacagctgc atgtccgatt
     1801 ccgcctggcc gatggcgtct tcgcgcgtgt agccgagcag gcggatgaag cccgcgttga
     1861 aatcgaggat ggtggagtcc gcgttgcgga cgatgaggat ggcgtccggg ctcgagtgaa
     1921 agattcggga gaaccgggct tcggagctgc gcagctcgcg ctccgatgcg atctggtcgt
     1981 ccatcagccg gcaggtcacg atggctccgc caacgccgtc gggcgtgcgc aggccggcga
     2041 tccgcgccgc caccgggaag tcgctgtcgc ggtcggggcg cagcaggtga agaccggaga
     2101 gctctgccac ctcgcccttc ttgcgggagt tcttcagggc ggtgatgaat cgctcgcggt
     2161 cgttcagctc gacgaggtcg gtgatcacgg cctcgttgag gtccgcgtcc ccggcaaggc
     2221 aggaagtggc caccgggttc gcgtagttga tcttgccgtg cttggagacc atcaggatga
     2281 ggtcgttgct cgattccagg gcctgacgga actcggccac ggacttcacc agggagtcct
     2341 gggcgccgag ggcggaagag acgtccacca gttgcaggca gagccggccg ctttcgggct
     2401 ccatggcccg gacgtggccg gcgacccaga cctcctcccc gtcggcgcgg acaaggcagg
     2461 aggagacgtc gatcacgacg ccgcggtcga gctcctcgcg gcggctttcc agctcggccc
     2521 agaggtcttc ccgggcaacg tcgctcagga gggtgcctgc catggagtcg ctgcctcggt
     2581 cgatctgggc ggcgaacgcc gcgtttaccc agacgatacg gcccgttgcg tcgatgaccg
     2641 cgctgccgca gggcgaggac tccagggcgt tgcgagctgc ctcgtccagc gtcgatcggt
     2701 ttgcgcggcg cgccatgagc aggggcacgg cggcccatag cagggcgagc gcccagagcg
     2761 tgccgacggt cagcggattg tgatggtaaa tacccaccac aagccctgcc gagatgcata
     2821 cgagtggtcc tagggcgacc agaagctgtc gtacacgttt gccctgcatg tctttgttgt
     2881 tcccggatcc cggcgatcgc tatgcttagc ggctgtccgc gcgtggtctg agatttcttc
     2941 catgaattac acgctgagcg tatccaccgg tcgtcaaggc ctctatgaca tcacgcacaa
     3001 agttgtcgaa tgtctaggca ggtccggcat aagtgagggc ttggtcactg tgttcgtccg
     3061 ccacacctcc gcgagcctgc tgattcagga aaatgcggac ccgagcgccc gcgaggatct
     3121 ggaatcgtgg ctgaaccggc tcgtaccgga ggtggacgag ttgtataccc atcgggcaga
     3181 gggaccggac gacatgcccg cccacatcaa atcggca
//
LOCUS       DBNHOX010000417         2175 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_547454, whole genome shotgun sequence.
ACCESSION   DBNHOX010000417 DBNHOX010000000
VERSION     DBNHOX010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_547454"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="AC16UZ_09295"
     CDS             <1..394
                     /locus_tag="AC16UZ_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin reductase domain-containing protein"
                     /protein_id="HIR6246918.1"
                     /translation="IDLVMGAPYTTDLLYDDLFRRLADEHDNFRYHTAVSREGADGGR
                     GLYVDDLLRQRLEEFRPLLGGDRTLIYVCGLTGMRISLYRLLVEENLADGYLTVDASL
                     QDTDPGGWSAVDVRRGIKHSDRCLIEVY"
     gene            complement(420..1181)
                     /locus_tag="AC16UZ_09300"
     CDS             complement(420..1181)
                     /locus_tag="AC16UZ_09300"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246919.1"
                     /translation="MELGLEGKRAIILGGSRGIGWYTARALAVEGCSVALCARDADGV
                     QAAVDALSEDPGGSHFGAAADLTDGDGTRAFVREAITALGGLDLLIHNASGFDRSGDE
                     EGWQRSFQVDVMAGVRAVEEALPALKESDAASITFIGSMASKFYFGRRSSYGPAKAAM
                     RAYANELAQSFGRDGIRANTISPGAVHFPGGSWDRVKEAQPDFYRNVEKAIPLGRLGT
                     GEEIARIVAFVASPAGIWINAAHIAADGGQVAAVD"
     gene            complement(1221..>2175)
                     /locus_tag="AC16UZ_09305"
     CDS             complement(1221..>2175)
                     /locus_tag="AC16UZ_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR6246920.1"
                     /translation="YQAMKALELNVCLDIKMSATAKLAHFVLPPRLGLESPAISLPNE
                     NIWFYGFSTGFPEPYAMYQPQLVEPPAGSDLLEEWEVFYGVAQRMGLGLTFAGEGLDM
                     NRKPTTDELFEIMCKGSRIPLEEVKRYPHGHIFSDPSITVLPKEEGCEDRLDVGHELM
                     MRELAEVRAEPLSGHGGYAGERAFTHRLVSRRMNDVYNSSGRDIPRLVRNHRYNPAYM
                     NPDDIEALGLHDGDVVEIASDHAKILGIVEAAPDIRPGVVSMAHAFGDAPEHDEDVFY
                     IGSNTGRLTDVERNYDPITGMPRMSAIPVNVRRGDQSLASR"
BASE COUNT          326 a          703 c          751 g          395 t
ORIGIN      
        1 catcgatctg gtcatggggg cgccgtacac gaccgatctg ctctacgacg atctgtttcg
       61 ccgcttagcg gacgagcacg acaatttccg ctatcacacg gcggtaagcc gggagggcgc
      121 cgatggcggt cggggtctct acgtggacga cctgctgcgg cagcgtctcg aggagttccg
      181 gcctctgctc ggcggggatc gcacccttat ctacgtgtgc gggctgaccg gcatgcgcat
      241 cagcctctac cgcttgctgg tggaggagaa cctggcagac ggctatctca ccgtggacgc
      301 gtcgctgcag gataccgatc ccggcggctg gtcagcggtg gacgtccgcc ggggcatcaa
      361 gcactcggat cgctgcctga tcgaggtcta ttgacggcgg aacccgcgtc cgtcggggct
      421 cagtcgacgg cggccacctg accgccgtcg gcggcgatgt gggcggcgtt gatccagatg
      481 ccggcggggc tcgccacgaa ggcgacgatc cgggcgatct cttccccggt gccgagcctt
      541 cccaggggaa tggccttctc cacgttgcgg tagaagtcag gctgcgcttc cttcacccgg
      601 tcccaggaac cgccgggaaa gtgcacggcg ccgggcgaga ttgtgttggc gcgaatgccg
      661 tccctgccga agctctgggc cagctcgttg gcataggcgc gcatggcggc cttggcgggc
      721 ccgtaggacg agcgccggcc gaaatagaac ttgctggcca tggagccgat gaaggtgatg
      781 ctggcagcgt cgctttcctt gagcgcgggc agtgcttcct ccaccgcccg cacgccggcc
      841 atcacgtcca cctgaaagct gcgctgccag ccctcctcat cgccgctgcg gtcgaagccg
      901 ctggcgttgt ggatcagcag gtccagccca ccgagggccg tgatggcttc gcgcacgaag
      961 gcccgggtcc cgtccccgtc ggtcaggtcc gccgccgcgc cgaagtggga tccgccagga
     1021 tcttcggaaa gggcgtccac cgcggcctgc acgccgtcgg catcgcgcgc gcagagggcc
     1081 accgagcagc cctcgaccgc cagcgccctc gccgtgtacc agccgatgcc gcggcttccg
     1141 cccagaatga tggcgcgctt accttcgagt cccagttcca tgcgttttcc tttgttcgtg
     1201 cccttggtcg tgcggtcgcc tcagcgggaa gcgaggctct ggtcgccgcg ccggacgttc
     1261 accggaatcg cgctcatgcg cggcatgccg gtgatggggt cgtagttgcg ctccacgtcc
     1321 gtgaggcggc cggtgttcga gccgatgtag aaaacgtcct cgtcgtgctc gggggcgtcg
     1381 ccgaaggcgt gagccatgga caccacgccg gggcgtatgt cgggcgctgc ctccacgatg
     1441 ccgagaatct tcgcgtggtc cgaggcgatc tcaacgacgt ccccatcgtg cagccccagg
     1501 gcctcgatgt cgtcgggatt catgtacgcc gggttgtagc ggtggttgcg caccagccgg
     1561 gggatgtcgc gcccggagga gttgtagacg tcgttcatcc tgcggctgac gaggcggtgg
     1621 gtgaatgccc gctcgccggc gtagcccccg tggcccgaca ggggttcggc ccgcacctcg
     1681 gcgagttccc gcatcatcag ctcgtgtccc acatccagac ggtcctcgca gccttcttcc
     1741 tttggcagca cggtgatgga tggatccgag aaaatgtggc cgtgggggta tcgtttgacc
     1801 tcctcgagcg gtatccggga acctttgcac atgatctcga acagctcgtc cgtggtgggc
     1861 ttgcggttca tgtccagccc ctccccggcg aaggtgagcc caagccccat gcgctgggcg
     1921 acgccgtaga agacctccca ctcttccagc aggtcggagc cggctggcgg ctcgacgagc
     1981 tggggctggt acatggcgta aggttcgggg aacccggtgg agaagccgta gaaccagatg
     2041 ttctcattcg gcaacgagat ggcgggcgat tccaggccga gccggggcgg cagcacgaag
     2101 tgggcgagct tggcggtggc cgacatcttg atgtccaggc agacgttgag ctcgagtgcc
     2161 ttcatggcct ggtag
//
LOCUS       DBNHOX010000418         2052 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_997849, whole genome shotgun sequence.
ACCESSION   DBNHOX010000418 DBNHOX010000000
VERSION     DBNHOX010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_997849"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="AC16UZ_09310"
     CDS             <1..569
                     /locus_tag="AC16UZ_09310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6246921.1"
                     /translation="TETTVESWDYTMDVLAKGVFLGIKHAARIMREQGEGGAIINTAS
                     IAGLSGDAGPMIYSAAKAAVISLTKSAAVELAPDRIRVNAICPGFIATPLADGGRRDE
                     TRERFATSQPWPEHGDGTHIAGAAVFLAGADSRFVTGEALIVDGGLTAAGPELSKRFP
                     RASGRHARVSGVTKGSTGEAPELKRLEE"
     gene            648..>2052
                     /locus_tag="AC16UZ_09315"
     CDS             648..>2052
                     /locus_tag="AC16UZ_09315"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6246922.1"
                     /translation="MAHDLVIRGGTIADGSGADPVPGDVAVNGTTITEVGNVEGAGRR
                     EIDARGLLVTPGFVDTHTHLDAQIGWDPMLTPVSWHGVTTALLGNCGVTFAPCHASDR
                     DFLAGMMETVEDIPKDAILGGLPWSWEDYGGYLDALQDLNPAINVAGLVGHCAVRYYV
                     MGERSIDDNSTAEERQRMAEVVADAVRAGAVGFSTSRFLGHYIPDGRHVPGTHADHDE
                     LVQIAAEVGKLGGLMQNVLNLGGDFEGEISLLAAEARASGQRVLFSNTAGKTQAWGDK
                     LNARISALRAEGLDINGIAIPRGSGFVTGLQAMLVWQGPTWGQLAKQDLSGRLQAIAD
                     PEFRARLIAEAEENPLRFDTRQMFWLGDGERPDYTCDGRNSLQALADANDELPVETFL
                     RLSRETAGKALFTIRFFNQNVDALAELISTDWCLPGLGDAGAHVSQIMDSGWATFVLS
                     HWARDAGLYTVGEAVRRI"
BASE COUNT          358 a          689 c          691 g          314 t
ORIGIN      
        1 tgaccgagac cacggtagag tcatgggatt acaccatgga cgtgcttgcc aaaggcgtct
       61 tcctggggat caagcacgcc gcacggatca tgcgtgagca gggcgaaggc ggtgccatca
      121 tcaacaccgc gtccattgca gggttgagcg gcgatgccgg cccgatgatc tactctgcgg
      181 ccaaggccgc cgtcatcagc ctcaccaagt ccgccgcggt ggagctggca ccggaccgga
      241 ttcgcgtgaa cgccatctgc ccgggcttca tcgccacgcc gctggccgac ggcggccggc
      301 gggatgaaac cagggagcgc tttgccacgt cacagccctg gcccgagcac ggcgatggca
      361 cccacattgc cggggcggcc gtgttcctgg ccggggctga ttcgcgattc gtcaccggtg
      421 aagcgctcat cgtcgatggc gggctgacag ccgcgggacc cgagctctcc aagcgctttc
      481 caagggcctc cggccgccac gcccgggtga gcggcgtcac caagggcagc acgggagaag
      541 caccggagct caagcgtctg gaggagtaga gctcggccat ccttgcccgc agcaagccgg
      601 ttgatctagc atcgatggtt tgcgtttgac gataacggag gcaacccatg gcgcacgacc
      661 tggtaatacg cggcggcacc atagccgacg gcagcggagc ggacccggtc cccggtgacg
      721 tggcggtgaa cggcacaacg atcaccgagg ttggcaacgt ggagggcgcc ggccgccgcg
      781 agatcgacgc cagggggctt ctcgtgacgc ccggtttcgt agacacccac actcacctgg
      841 atgcccagat cggctgggat cccatgctca ctccggtaag ctggcacggc gtcaccaccg
      901 cccttctcgg caactgcggc gtcaccttcg ctccctgcca cgcatcggac cgcgacttcc
      961 tggccgggat gatggagacc gtggaagaca tccccaagga tgccatcctc ggcgggctgc
     1021 cctggtcgtg ggaagattac ggcggttatc tggacgccct gcaggatctg aacccggcca
     1081 tcaacgtggc cggcctcgtc ggccactgcg ccgtgcgcta ctacgtgatg ggcgagagat
     1141 ccatcgacga taactccacc gccgaggaga ggcagcggat ggccgaagtg gtggccgacg
     1201 cggtgcgggc gggagccgtc ggcttctcca cgtcccggtt cctcgggcac tacatcccgg
     1261 acggccgcca cgtcccgggc acccacgccg accacgacga gctcgtgcag attgcggcag
     1321 aggtcggcaa gctgggcggg ctcatgcaga acgtgctgaa cctcggcggt gatttcgagg
     1381 gagagatttc cctgctggcc gccgaggcgc gggcgagcgg gcaacgggtg ctgttcagca
     1441 acacggcggg caagacccag gcctggggcg acaagctcaa tgcccgcatc agcgcgctgc
     1501 gcgccgaggg gctggacatc aacggcatcg ccataccccg cgggtccggc ttcgttaccg
     1561 ggcttcaggc catgctcgtc tggcaggggc ccacctgggg gcaactcgcc aagcaggacc
     1621 tctcaggccg actgcaggcc atcgccgacc ccgagtttcg cgcccggctg atcgcggaag
     1681 ccgaggaaaa ccccttgcga ttcgacacgc gccagatgtt ctggctgggc gacggagaac
     1741 gtcccgacta cacctgtgac ggtcgcaaca gcctgcaggc cctggcggac gccaacgacg
     1801 agctgcccgt ggagacgttc ctgcgcctga gccgggagac tgcgggcaag gcccttttca
     1861 ccatccgctt cttcaatcag aacgtcgacg cgctggccga gctgatctca acggactggt
     1921 gcctgcccgg cctgggcgac gcgggcgccc acgtgagcca gatcatggac tccggctggg
     1981 ctaccttcgt gctctcccac tgggcgcgtg acgctggcct gtacacggtg ggtgaagccg
     2041 tgcgccggat ca
//
LOCUS       DBNHOX010000419         2466 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_804568, whole genome shotgun sequence.
ACCESSION   DBNHOX010000419 DBNHOX010000000
VERSION     DBNHOX010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_804568"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="AC16UZ_09320"
     CDS             complement(<1..461)
                     /locus_tag="AC16UZ_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIR6246923.1"
                     /translation="MLKLIPLALLSLVLGSCANEPARPPAPDPGAPVVIAKSPNDERS
                     YRYLVLDNELRVLLVSDPAADMAAASLVVLRGSFHEPAEFAGLAHFLEHMLFIGTEKY
                     PEVDSFKQFVAAQGGSSNAYTANDHTNYFMEVRTEAFREALDRFAQFFISPL"
     gene            complement(499..825)
                     /locus_tag="AC16UZ_09325"
     CDS             complement(499..825)
                     /locus_tag="AC16UZ_09325"
                     /inference="COORDINATES: protein motif:HMM:NF019301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HIR6246924.1"
                     /translation="MTVSPPLPLGSTASWFLASWTEFLGGILLLFGLGTRLVAKAKDI
                     LREHGDYRYLNGSGSIVKLNGGIEFAAIYIAMLLALLVIGGGRYLSVDYDVGLLGRTR
                     ASAQRE"
     gene            958..1839
                     /locus_tag="AC16UZ_09330"
     CDS             958..1839
                     /locus_tag="AC16UZ_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246925.1"
                     /translation="MAGLTLDDLAQALLAGSEPWTDPGALAARLDPDIPEDWRNLAAV
                     VVAAGATGTIGVGGGQGAGKSTLAAAMVEASRLAGRKAQACSLDDFYLTRQERLALAR
                     TVHPLFVTRGVPGTHDVDLALSVLRGLEGVGVTRVPVFDKGRDDRAPGPEWRAVEGPV
                     ESIVFEGWCLGAPPEPAERLIEPMNSLEREEDANRTWRSHVNDVLGESYQRLWSAMDR
                     LLYLQVPDMAAVHDWRAQQELALPPSRRMSGGQLDRFLAHYERLTRWMLETLPERADV
                     VVELGRDHRISGVRRNS"
     gene            1920..2447
                     /locus_tag="AC16UZ_09335"
     CDS             1920..2447
                     /locus_tag="AC16UZ_09335"
                     /inference="COORDINATES: protein motif:HMM:NF017269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HIR6246926.1"
                     /translation="MYASHTAKTLLVVALVAPALAVAGTSYDRARVVSVEPLYETVSY
                     EVPREECRMEEVRHTRSGSHRSYTAPIVGAIVGGAIGNAVGHKKRNKQIGTVVGAVLG
                     GSIGADIARRGHHHGHGPEYGLERVCRTVSDWHEEQRLLGYRVTYRYAGETYITQLDE
                     APGSSLPVRVQVTPV"
BASE COUNT          431 a          759 c          844 g          432 t
ORIGIN      
        1 agcgggctga tgaagaactg cgcgaatcga tcgagggctt cccggaaggc ctcggtcctg
       61 acctccatga agtagtttgt atggtcgttg gccgtgtacg cgttggacga ccctccctgg
      121 gcggcgacga actgcttgaa cgagtccacc tccggatact tctcggtgcc gatgaacagc
      181 atgtgctcca gaaagtgggc gaggccggcg aactcggcgg gctcgtgaaa gctccctcgc
      241 agcacgacca gcgaggcagc ggccatgtcc gctgccgggt cggatacgag cagcacccgc
      301 agttcgttgt cgagaacgag gtagcggtag ctcctttcgt cgttcggact cttggcgatg
      361 acgaccggag ccccgggatc gggggccggc gggcgcgccg gttcgttggc gcacgatccc
      421 agcaccagtg aaagcagcgc gagcggtatg agtttcagca cgtttcggtt tccagttgtt
      481 gagcggtccg ggcggcgatc attcccgttg tgcggacgcg cgagttctcc cgagcagccc
      541 gacatcgtag tcgacgctga gataacggcc acctccgatc accagcaacg ccaggagcat
      601 ggcgatgtag atggcggcaa attcgatgcc gccgttcagc ttgacgatgc tgccgctgcc
      661 gttgagatac cgatagtcgc cgtgttctcg caggatgtcc tttgctttgg cgaccagccg
      721 ggtgccaagt ccgaacagca gcaggatgcc tcccaggaac tcggtccagg aagccaggaa
      781 ccacgaagcc gtcgagccga gcggcaatgg tggcgagacg gtcatgccag tcagctacgg
      841 ttgctatgat tggctggatg aggcttctcc catgtttttc agaattagac tcgaaggcgg
      901 agcagggttc cccggagggc ggtccatgat gcacaaaccg tcaggtgatg gaaaaccatg
      961 gcgggtctga cactcgacga cctggcccag gcgctgctcg caggttccga gccatggacg
     1021 gatcccgggg cgctggcggc ccgcctcgat cccgacattc ccgaagactg gcgcaatctg
     1081 gcggccgtcg tcgtcgctgc gggagcgacg ggcaccatcg gcgtcggcgg gggccaggga
     1141 gcgggcaagt cgactctggc tgcggcgatg gtcgaagcca gccgcctggc cggacgaaag
     1201 gcgcaggcct gctcgctcga tgatttctat ctcaccaggc aagagcgtct ggctctggca
     1261 cgaaccgttc atccgctctt cgtgacccgg ggcgttcccg gtacccatga cgtcgatctg
     1321 gcgctgtccg ttctgcgcgg gctcgagggt gtcggcgtca ccagggtgcc cgtattcgac
     1381 aaaggccggg acgatcgtgc gcccggcccg gagtggcgtg cggtcgaggg cccggtagag
     1441 agcatcgtct tcgagggctg gtgcctgggc gccccgcccg agcctgccga acgtctcatc
     1501 gagccgatga actcgctcga gcgcgaagag gatgcgaacc ggacctggcg ctcccacgtg
     1561 aacgatgttc tgggcgagag ctatcaacgg ctttggagcg cgatggatcg cctgctctac
     1621 ctccaggttc cggacatggc cgccgtgcac gactggcgcg cacagcagga gctggcgctg
     1681 cccccgtcca ggcgcatgtc gggcggtcag ctcgaccggt tccttgccca ctacgagcgg
     1741 ctgacgcgct ggatgctcga aaccctgccc gaacgggcag acgtggtcgt cgagctcggt
     1801 cgcgaccatc ggatctcggg cgttcgcagg aattcctgac ccgtaggccg tattcatgat
     1861 ccgttcagtc gtcggtcctt aatctggcgg ccgtcattgc agtcgaatgg agaatgacga
     1921 tgtatgcatc ccacacagcg aaaactctgc tggtcgtcgc cctggtggcg ccggcactgg
     1981 cagtggcggg cacgtcctac gatcgggcca gggtggtttc cgtcgagccc ctttacgaaa
     2041 ccgtctccta cgaggtcccg cgcgaggagt gccggatgga ggaagtgcgg cacacccgaa
     2101 gcgggtcgca tcgttcgtac acggcgccca tcgtcggcgc catcgttggc ggcgccatcg
     2161 gcaacgccgt gggccacaag aagcgcaaca agcagatcgg caccgtggtg ggagccgtgc
     2221 tcggcggcag tatcggcgcg gacatcgcaa ggcgcggtca ccaccacggg cacggaccgg
     2281 aatacggtct ggagcgggtt tgccggaccg tcagcgactg gcacgaggag cagcggttgc
     2341 tcggttaccg tgtcacctac cgctacgcgg gggagaccta catcacgcaa ctggacgagg
     2401 cgccgggttc gagccttccc gtgcgcgtgc aggtcacgcc ggtgtgaccg gggcaacgtt
     2461 agaagg
//
LOCUS       DBNHOX010000420         1598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_98043, whole genome shotgun sequence.
ACCESSION   DBNHOX010000420 DBNHOX010000000
VERSION     DBNHOX010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_98043"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..648
                     /gene="rfbC"
                     /locus_tag="AC16UZ_09340"
     CDS             106..648
                     /gene="rfbC"
                     /locus_tag="AC16UZ_09340"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003173486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIR6246927.1"
                     /translation="MRITQTRLPGVLVIEPNIYGDERGFFVETFQAARYRDLAGIELP
                     FVQDNHSRSRYGVLRGMHAQRNHPQGKLVRVARGEVFDVALDIDPESPTFGQWAGATL
                     SDTNGQQMWIPPGYAHGFVVLSDVADFEYKCTDYYHADDEIGVLWNDPDAAIDWPIDD
                     PVLSDKDKALPTLAELRSAR"
     gene            645..1520
                     /gene="rfbD"
                     /locus_tag="AC16UZ_09345"
     CDS             645..1520
                     /gene="rfbD"
                     /locus_tag="AC16UZ_09345"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR6246928.1"
                     /translation="MTVLVLGANGQVGTHLRDCLPDAVFWTRDKADLSRPTQLEDAVL
                     AQKPSVIVNAAAYTAVDRAEEETDLAWTVNAEAPAALARAARELGAILVHFSTDYVFD
                     GTKAGPYLDTEPTRPQSAYGATKLAGELAVATLCPTHWIFRTSWVFSEHGGNFVKTML
                     RLGVERDELGVVDDQTGRPTYAGHIASAVAEVIGTGKGQALPWGIYHLTGGKPVSWCT
                     FAREIFRRAGDRGLMSSRPSVKAITTDEYPTPAKRPANSVLQPSAALREQLSTLPDWE
                     AGLDTVLAMIEAERS"
BASE COUNT          280 a          540 c          535 g          243 t
ORIGIN      
        1 ctggcgggcc ggctggatca gcgacgatgc gctcgcttcc ctcgcagcgc cgcttgcgaa
       61 gagcggctac ggcgattacc tgacgggttt gctggagacg ggggcatgag gattacgcag
      121 accaggctgc ccggcgtgct cgtcatcgag cccaacatct acggagatga gcggggcttc
      181 ttcgtggaga cgtttcaggc cgcgcggtac cgggacctcg ccggtatcga gctgcccttc
      241 gtgcaggaca accactcccg gtcccgctac ggcgtgctgc gcggcatgca cgcccagcgc
      301 aaccatcccc agggcaagct ggtgcgcgtg gcccggggcg aggtgttcga cgtggctctc
      361 gacatcgatc ccgaatcccc gacgttcggc cagtgggcgg gcgcgacctt atccgatacg
      421 aacggccagc agatgtggat cccgcccggc tacgctcatg gctttgtagt gctctcggac
      481 gtggccgact tcgagtacaa gtgcacggac tactatcacg ccgacgacga gatcggcgtg
      541 ctctggaacg accccgatgc ggccatcgac tggcccatcg acgaccccgt tctctccgat
      601 aaagacaagg ccctgcccac gctggccgag ctgcggtcgg ccagatgacc gttttagtac
      661 tcggcgccaa tggccaggtg ggcacgcacc tgcgcgactg cctgccggat gccgtcttct
      721 ggacaagaga caaagccgat ctctcccggc cgacccagct ggaggacgcg gtgttggcgc
      781 agaagccgtc cgtcatcgtc aacgccgccg cctacaccgc cgtggaccga gccgaagagg
      841 aaacggacct cgcctggacg gtcaatgcgg aggcgccggc cgcgttggcc cgcgccgccc
      901 gggagctggg cgccatcctt gtgcacttct ccaccgacta cgtcttcgac ggcacaaaag
      961 cggggcccta cctggacacg gagccaacga ggccccagag cgcgtacggc gccaccaagc
     1021 tcgccggcga gctggccgtg gcgacgctgt gcccgacgca ctggatcttc cgaaccagct
     1081 gggtgttcag cgagcacggt ggcaacttcg tgaagaccat gctgcggctg ggcgtcgagc
     1141 gggacgagct cggcgtggtg gacgaccaga ccggccgccc cacctacgcc ggccacatcg
     1201 cctcggccgt ggccgaggtc atcgggaccg gcaaggggca ggcgctgccc tggggcatct
     1261 atcacctgac aggcggcaag ccggtctcct ggtgcacgtt cgcgcgggag atcttccgcc
     1321 gcgccggcga ccgcgggttg atgtctagcc ggccgtcagt gaaggcgatc accaccgacg
     1381 agtacccgac cccggcgaag cggccggcca actcggtcct gcagccaagc gctgcgttac
     1441 gcgagcagct gtctaccctg ccggactggg aagcggggct ggacacggtg ctcgcgatga
     1501 tcgaggctga gcgctcgtag ccgcgatccg gaatcgtaac cggctcagcg ttaatctcgg
     1561 caaatccagg gagcaaaagg aagagggcca aatggcac
//
LOCUS       DBNHOX010000421         3662 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1062318, whole genome shotgun sequence.
ACCESSION   DBNHOX010000421 DBNHOX010000000
VERSION     DBNHOX010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1062318"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..986)
                     /locus_tag="AC16UZ_09350"
     CDS             complement(63..986)
                     /locus_tag="AC16UZ_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR6246929.1"
                     /translation="MARASVFDNQEKQAIVRRILVGMREAGCNEVAYLDDGHGIVRTA
                     LDDVREPFIRKPVATTETANALDTINAARALRAEGCDVVLTLGGDGTNRAFALGWPDP
                     PLIPISTGTNNVFPVLVEATIAGAAAGLIASGQISLADVATQHKAIHVTIVGERDDLA
                     LIDAVLSSERFVGARALLAPETLQTALVTRADPAAVGITSIGGLLKPVADAEDGGLLM
                     QLGGTGGSVVNAPIAPGYYRRVRVSELRRVGFAETIEVTGPGVLAFDGERERSLKPGQ
                     RATMSVHRSGPWVVDVTKTLMLAAEKGCFRR"
     gene            complement(1049..2047)
                     /locus_tag="AC16UZ_09355"
     CDS             complement(1049..2047)
                     /locus_tag="AC16UZ_09355"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020300037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6246930.1"
                     /translation="MAEAQQKPRELPYVSAITDGVRQVLLEQPNSFVAGEDVGGVGGV
                     YGYYQGLQKEFGPERVYDTPISEEGIVGLGVGAAATGCRPIIDLMFMDFLGECMDEVS
                     NQLAKMRYMFGGHATLPVTMLTMAGAGASLAAQHSQSLEAWICHLPGLKVCMPSSPYD
                     AKGLIISAARDDNPVIVVLNKMSLAITGEVPEEPYAIPLGEANIVRPGGNFTLIATGR
                     MVYEAMTAAEKLKDLGIDTEVIDPRTIQPFDTQTVIESVKKTHRVMIVHEAVRFGGIG
                     GEIAAQIQELAFDYLDAPVARVGAPFSPVPFSPALEAVYVPNADRIVAEAKKLLGL"
     gene            complement(2061..3032)
                     /locus_tag="AC16UZ_09360"
     CDS             complement(2061..3032)
                     /locus_tag="AC16UZ_09360"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019097910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR6246931.1"
                     /translation="MTTQISNETLLDIHRRMVRIRIFEETAGKMMEDGKIPGALHLYV
                     GEEAVAAGVTVHLSPSDWVTSTHRGHGHLIAKGGEFDKMMAELFGRATGYNKGKGGSM
                     HICNMELGMLGANGIVGGGPPIAMGAGFSNKFRKTKDVSISFFGDGASNEGAFHEAAN
                     MAALYKLPVIFVCENNGYGEYTPQAKHQAIVDVADRAPGYGMPGVIVDGMDAVAVYEA
                     AGEAIARARAGDGPTLLECKTYRFYDHVGVRGMGMNYRTEEEVEQWKQRDPIVLLEQR
                     LAEQDILSKEAAQAVHDQVTREVDAAVKFANESPMPDPSALLEDVYA"
     gene            3207..>3662
                     /locus_tag="AC16UZ_09365"
     CDS             3207..>3662
                     /locus_tag="AC16UZ_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6246932.1"
                     /translation="MTFAKRLADERPDEIALRDPRNAYTWGEVDATLNRVANGLLAMD
                     LGPDHRVAVFAENAAETAFANLGGLLAGASVVPVNFHLTADEVAYILNDSDARVLFAD
                     ARTAERALAAASQSNVHTVIAWDVPAEDGLTPWADWLAGNSADDPPDDMA"
BASE COUNT          576 a         1204 c         1216 g          666 t
ORIGIN      
        1 gtgggcattg cgaaaccttc ctttcgttgt tcttcggcgt cacccgcgcc gaacgcgaca
       61 tcctagcgcc tgaaacagcc tttttcagcc gcgagcataa gggtttttgt gacatcgacc
      121 acccacgggc cggagcggtg gacggacatc gtcgctcgct ggccgggttt gagggaacgt
      181 tcgcgctcgc cgtcgaaggc cagcacgccg ggtccggtca cctcgatcgt ctctgcgaac
      241 cccacccgac gcagctcgct cacccggacc cgccggtagt agccgggggc gatgggcgcg
      301 ttgaccacgc tgccgcccgt gccgccgagc tgcatcagca ggccgccgtc ctcggcgtcg
      361 gccacgggct tcaggaggcc gccgatggac gtgatgccca ccgccgccgg atcggcccgg
      421 gtaaccagcg ccgtctgcag cgtctccggc gcaaggagcg cacgggcgcc gacgaagcgc
      481 tcgctgctca gcacggcgtc gatgagggcg aggtcgtcgc gctcgcccac gatggtcacg
      541 tgtatggcct tgtgctgggt tgccacgtcg gcgagcgaga tctggccgga ggcgatgagc
      601 cccgcagcgg cgcctgcgat ggtggcctcc accagaaccg gaaagacgtt gttggttccc
      661 gtggagatag gaatcagggg cggatccggc cagccgagcg cgaaagctcg attggttccg
      721 tcgccgccga gcgtgagcac cacgtcgcaa ccttccgctc gcagtgcccg ggccgcgttg
      781 atggtatcca gggcgttggc ggtttccgtg gtggccaccg gcttgcggat gaagggctcg
      841 cgcacgtcgt ccagggccgt gcgaacgatg ccgtggccgt cgtccagata ggcgacctcg
      901 ttgcagcccg cctcgcgcat gcccaccagg atgcggcgga cgatggcctg cttttcctgg
      961 ttgtcgaaga cggacgcacg cgccaccagg cgccggacat ctttgcccga cgccgggttc
     1021 gcgatgatgc ctatggtgcc cagggtcgct ataagcccag cagcttcttc gcttcggcta
     1081 cgatgcgatc ggcgttgggc acgtaaacgg cttccagcgc cgggctgaac ggcaccggcg
     1141 agaagggcgc gccaacccgc gcaacaggcg catcgaggta gtcgaacgct agctcctgga
     1201 tctgcgcggc gatctcgccg ccgatgccgc cgaagcgcac ggcctcgtgc acgatcatca
     1261 cccgatgggt tttcttgacc gactcgatga ccgtctgggt gtcgaatggc tgaatggtgc
     1321 gtggatcgat gacctcggtg tcgattccga gatccttcag cttctccgcc gcggtcatgg
     1381 cttcgtagac catgcgcccg gtggcgatga gcgtgaagtt cccgcccggc ctgacgatgt
     1441 tcgcctcccc gagggggatg gcgtacggct cctcgggtac ctcgcccgtg atggccaggg
     1501 acatcttgtt cagcaccacg atgaccggat tgtcgtcccg cgctgcagag atgatcagcc
     1561 ccttggcgtc gtaggggctg gagggcatgc agaccttcag gcccggcagg tggcagatcc
     1621 acgcctccag gctctgggaa tgctgggctg ccaggctcgc gccggcgccg gccatggtga
     1681 gcatggtgac gggaagggtc gcgtggcctc cgaacatgta gcgcatcttg gcgagctggt
     1741 tgctcacctc gtccatgcac tccccgagga agtccatgaa catcaggtcg atgatgggcc
     1801 ggcagcccgt cgcggcggcg ccgacgccga gcccgacgat gccctcctcg gagatgggtg
     1861 tgtcgtaaac ccgctcgggt ccgaactcct tctgcaggcc ctggtagtag ccgtaaacgc
     1921 cgccgacccc gccgacgtcc tcgccggcga cgaaagagtt gggttgctcc agcagtacct
     1981 gccggacgcc atcggtgatg gcggagacgt aaggcagttc cctcggtttc tgttgtgctt
     2041 cagccatggt ttttctccgc ttaagcgtac acgtcttcga gcagcgcgct cgggtccggc
     2101 atggggcttt cgttggcgaa cttgaccgcg gcatcgactt cacgggttac ctggtcgtgc
     2161 acggcttggg cggcctcttt ggagagaatg tcctgttccg ccaggcgctg ctccagcagc
     2221 acgatgggat cgcgctgctt ccactgctcc acttcttcct cggtgcggta gttcatcccc
     2281 atgccgcgca cgccgacgtg gtcgtagaag cggtaggtct tgcactccag cagggtgggg
     2341 ccatcaccgg cgcgggcccg ggcgatggct tcgccggcgg cctcgtaaac ggccaccgcg
     2401 tccatgccgt ccacgatcac gcctggcatc ccgtagccgg gcgcccggtc ggccacgtcc
     2461 acgatggctt ggtgtttggc ctgaggcgtg tactcgccgt aaccgttgtt ctcgcagacg
     2521 aagatcacgg gcagcttgta gagcgcggcc atgttggccg cctcgtgaaa ggcgccctcg
     2581 ttgctcgcgc cgtcgccgaa gaaggagatg gagacgtctt tcgtcttgcg gaacttgttg
     2641 gagaagcccg cgcccatggc gatgggcggg ccgccgccga cgatgccgtt ggcgcccagc
     2701 atgccgagct ccatgttgca gatgtgcatg ctgccgccct tgcccttgtt gtaaccggtc
     2761 gcgcgaccga acagctcagc catcatcttg tcgaactcgc cgcccttggc gatcagatga
     2821 ccatggcccc ggtgggtgct ggtcacccag tcgctgggag acaggtgcac ggtgacgccg
     2881 gcggccacgg cctcctcgcc aacatagagg tgcagagcgc cggggatctt gccgtcctcc
     2941 atcatcttcc ctgcggtctc ttcgaatatg cgtatccgga ccatccgccg atggatgtcc
     3001 agcagcgtct cgttggaaat ctgcgtcgtc aaatatgcct cccctccaga gcgaccggag
     3061 taaaaagtct tcttggaccc gggtcctgcc cgcggccggg gcagtgacaa atcatagggt
     3121 cgtcctttat aacggaacat tcggcaggct taaacccgca aatcgcgctc cggtgggaaa
     3181 ggcgtgcggg cggagaggga gaatacatga cttttgccaa gcgcctcgca gacgagaggc
     3241 cggatgagat cgcgcttcgc gacccgcgca atgcctacac ctggggcgag gtcgacgcca
     3301 cgctcaaccg cgtcgccaac gggctgctcg ccatggatct ggggcccgat caccgcgtgg
     3361 cggtgtttgc cgagaacgcg gcggagacgg cctttgccaa cttaggcggc ctcctggcgg
     3421 gagcctcggt ggtgccggtc aactttcacc tcaccgcgga cgaggtcgcc tacattctca
     3481 acgactccga tgcccgcgtg ctgttcgcgg acgccagaac ggcggaacgg gcgctcgccg
     3541 cggcttccca gtccaacgtg cacacggtca tcgcctggga cgtgccggcc gaggacgggc
     3601 tcacgccctg ggcggactgg ctggcgggga actcggccga cgacccgccc gacgacatgg
     3661 ca
//
LOCUS       DBNHOX010000422         2172 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_868249, whole genome shotgun sequence.
ACCESSION   DBNHOX010000422 DBNHOX010000000
VERSION     DBNHOX010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_868249"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="AC16UZ_09370"
     CDS             complement(<1..1000)
                     /locus_tag="AC16UZ_09370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR6246933.1"
                     /translation="MLWLGLHLPNLGLEIFEAAAPGTPQVLVEDNRVILQNPAAVAAG
                     ITPGSTLATAHSIEPRLRHCNRDEIRERERLELLAGALYGFSSHVSTQPPSSLVLEAG
                     GSLELFGDLAKLEQAVRETCEALGHVTIMRTARTPSAAMTLARARQDDLKHVPLELTE
                     LATSMVERFANMGMHALGALIALPEDELAHRFGPELVNYLKRLTGRMPDPREAVTPAE
                     AFSSRLHLLEAVTGKDALLFPMQRLLGELQIWLVSRQLGAEQIVWTLDDHGSKDTAEL
                     TIRFARAQQQREAFLDITRLKLESAELPGDVLGIGLRAERLVPWQAGSRTLFQSQPG"
     gene            complement(1000..1794)
                     /gene="imuA"
                     /locus_tag="AC16UZ_09375"
     CDS             complement(1000..1794)
                     /gene="imuA"
                     /locus_tag="AC16UZ_09375"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HIR6246934.1"
                     /translation="MAEPSGKASRLLEHPALWRAGQLNQATEALSTGFPELDAHLPGR
                     GWPRAGLSEFLLDTTGAGELKLLAPLLKDLSRSEARWIAWVNPPFIPYAPALTALGID
                     VDRVLLIHPRDHKDALWAFERAARSGGCSVVLGWLDEKQLKVKDTRRLQLAARQGRAF
                     SCLFRPVRAAAESSMAELRLKMKALGSGKLGVSITKRRGGWPVTGLNLTVAEPRRPAE
                     IREQLSLWRSLQHPQDAGNEAELPVLGNPAPAGATHAPSSPGRLVH"
     gene            complement(1876..>2172)
                     /locus_tag="AC16UZ_09380"
     CDS             complement(1876..>2172)
                     /locus_tag="AC16UZ_09380"
                     /inference="COORDINATES: protein motif:HMM:NF015665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HIR6246935.1"
                     /translation="AGARVRAYDPAAMAEAGHLYDGQDGLTLCEARDGTLEGADALVV
                     VTEWNEFRSPDFLDVKARLKQPVIFDGRNLYDPDYLAQLGFDYYAIGRGKPSQT"
BASE COUNT          327 a          723 c          755 g          367 t
ORIGIN      
        1 gcccgggctg ggattgaaaa agcgtccggc tgccggcctg ccagggcacc aggcgctcgg
       61 cacgcaggcc gatgcccagc acgtcgccgg gcaattccgc gctttcgagc ttcagccggg
      121 tgatgtcgag aaaggcctct cgctgctgct gggccctggc aaagcggatt gtcagctcgg
      181 ccgtgtcctt cgatccgtga tcgtcgagcg tccagacgat ctgctcggca cccagctgcc
      241 ggctcaccag ccagatctgc agctcgccga gcagccgctg catggggaag agcagggcgt
      301 ccttgcccgt caccgcctcc agcaggtgca gccggctaga gaacgcctct gccggcgtca
      361 cggcctcccg gggatccggc atccggccgg tcagccgctt gagatagttc acgagctccg
      421 ggccgaagcg atgggcgagc tcgtcctccg gcagggcgat cagcgctccc agggcgtgca
      481 tgcccatgtt ggcgaagcgc tccaccatcg acgtggccag ctccgtgagc tccaggggga
      541 cgtgtttcag atcgtcctgt cgggcacggg ctaaggtcat ggccgccgag ggggttcggg
      601 ccgttcgcat gattgtgacg tgaccgagag cttcgcaggt ctcgcggact gcctgctcca
      661 gtttggccag atcgccgaac agctccaggc tgccgcctgc ctccagcacc agggagctcg
      721 gcggctgggt ggagacgtga gaggagaaac cgtagagcgc tccggccagg agctccagcc
      781 gctcccgttc ccgaatctcg tcccgattgc agtgccgcag ccgcggctcg atgctgtgag
      841 cggtggccag ggtgctgcct ggggtgatgc cggcggcgac cgcggccggg ttctgcagga
      901 tgacccggtt gtcctccacc agcacctggg gcgtgccggg cgctgctgct tcgaagattt
      961 cgagccccag gttggggagg tgcaggccga gccagagcat cagtggacga ggcggccggg
     1021 ggaagagggc gcgtgggttg cacccgccgg cgccgggttg ccgagaacgg gcagctccgc
     1081 ctcgttaccg gcgtcctgcg gatgctgcag gctgcgccag agcgacagct gctcgcggat
     1141 ctctgcgggc cggcggggtt cggccacggt caggttcagc cccgtgaccg gccagcctcc
     1201 gcgccgtttg gtgatcgaca ctcccagctt tccggatccg agagccttca tcttcagcct
     1261 cagctcggcc atggagctct ccgccgcggc ccttaccggg cggaacaggc aggagaacgc
     1321 acgcccctgc cgggcggcga gctgcagcct ccgggtgtct ttgaccttta gctgcttctc
     1381 gtcgagccag cccagcacca cgctgcaccc gcccgatcgc gccgcccgct cgaaggccca
     1441 cagagcgtcc ttgtggtccc gcggatggat gagcagcacc ctgtccacgt cgatgccgag
     1501 cgcagtgagg gccggggcgt aagggatgaa cggcgggttg acccaggcga tccagcgcgc
     1561 ctcgctgcgg ctcaagtcct tgagcagagg tgcgagcagc ttcagctcgc cggcgcccgt
     1621 ggtgtccagg agaaactcgc tcaggcccgc tctcggccag ccccggccgg gcagatgagc
     1681 atcgagctcg ggaaaacccg tgctgagcgc ctcggttgcc tggttcagct gcccggcacg
     1741 ccagagtgcg gggtgttcaa gaagtcggga ggcttttccg gacggctcgg ccatgctcgc
     1801 gctcgatacg gtcttttcat acaggggtcg gccagattac ctgtatggat atacagtatc
     1861 aagaggttcg aagcgtcagg tctggctcgg cttgccccgt ccgatggcgt agtagtcgaa
     1921 gcccagctgg gcgagatagt ccgggtcgta gaggttgcgg ccatcgaaga tcacgggctg
     1981 cttgagccgc gccttgacgt ccaggaagtc ggggctgcgg aactcgttcc actccgtgac
     2041 cacgaccagt gcgtccgcgc cttccagggt gccgtcccgc gcttcgcaca gggtgaggcc
     2101 atcctgcccg tcgtaaagat gcccggcttc cgccatggcg gcggggtcgt aggccctcac
     2161 cctggcaccg gc
//
LOCUS       DBNHOX010000423         2628 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_740598, whole genome shotgun sequence.
ACCESSION   DBNHOX010000423 DBNHOX010000000
VERSION     DBNHOX010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_740598"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="AC16UZ_09385"
     CDS             complement(<1..1442)
                     /locus_tag="AC16UZ_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015635058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR6246936.1"
                     /translation="MNHASALRQLRAALPEGAVLTDRHQLKPYETDGLTALRQMPWAV
                     ALPQTEAEVREVLRICAEHEVPLVPRGAGTGLSGGARPVSDGLVLGVSRLDRILEIDV
                     ENGVARVEPGVRNLAISEAASAYGLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGLT
                     VHNVLGLKVLTVEGDELQIGGKRLDSPGYDLLALMSGSEGMLAVITEITVRLLPIPPE
                     KRVLLGAFRTVAAAAEAVNQVISEGLVPAGLEMMDRLAIEAAEDFVHAGYPREAEAIL
                     LCEVDGVTEEVDNDVDLVRRVFETLGAFDVRIATDPAEQANLWKGRKAAFPAVGRISP
                     DYYCMDGTIPRYELARVLAEIASLATEFGLRVANVFHAGDGNLHPLILYDAGREGELA
                     KAEALGGKILELCVEVGGTVTGEHGVGVEKLDQMCAQFSPEELAQFFGVKACFDPGQT
                     LNPGKAIPTLARCSEVGGMHVHGGRLPYGNL"
     gene            1577..>2628
                     /locus_tag="AC16UZ_09390"
     CDS             1577..>2628
                     /locus_tag="AC16UZ_09390"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011534274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR6246937.1"
                     /translation="MTNSYLISNARIVNEGTITEGDLLVENGRISGIGKPAPAGTDVF
                     DANGAWLLPGMIDDQVHFREPGFEHKGTIATESRAAIAGGITSYMEMPNCNPLTVTHE
                     ALADKHVRAKDHSAANFAFYFGATNDNLEAIKTVDPALTCGIKVFMGASTGNMLVDDA
                     DTLEGIFAGTPLIVATHCEDTPTILANEERAREAFGDAIPFSEHPRIRSEEACWLSSS
                     MAVGLARRHGTRLHVLHLTTAREMALFEPGDLMGKQITAEACVHHLFFNESWYPDKGA
                     DIKCNPAIKGAADQEALLRAVAEDRIDVIATDHAPHTREEKDREYLRAPAGLPLVQHA
                     LSSLVEQVHRGVFDIET"
BASE COUNT          506 a          906 c          801 g          415 t
ORIGIN      
        1 agattgccgt acgggagacg accgccgtgg acgtgcatgc ccccgacttc tgagcagcgc
       61 gccaaagtcg ggatcgcctt gcccggattg agggtctggc cgggatcgaa gcaggccttc
      121 accccaaaga actgggcgag ttcctcgggc gagaactgcg cgcacatctg atcgagcttc
      181 tcgacgccaa caccgtgctc tccggtcacc gtgccgccca cctcgacgca aagctcgagg
      241 atcttgccgc caagggcctc ggctttcgcg agctcacctt cgcgccccgc gtcgtaaaga
      301 atcaatgggt gcagattgcc gtcgccggca tggaagacgt tggcgactcg aagcccgaac
      361 tccgttgcca gcgaagcgat ctcagccagc acgcgcgcga gctcatagcg gggtatggtt
      421 ccgtccatgc agtagtagtc cggagagata cgccccaccg cgggaaaggc ggctttgcgg
      481 cccttccaca gattggcctg ctcggccgga tcagtggcga tgcgcacgtc gaaagcacca
      541 agcgtttcga acaccctgcg aaccaggtcg acgtcgttgt ccacctcctc ggtcacgccg
      601 tcgacctcgc acagcagaat ggcttccgcc tcccgcgggt aaccggcgtg cacgaagtcc
      661 tcggccgcct cgatggcgag ccggtccatc atctccaatc ctgccggcac cagaccctct
      721 gagataacct gattcacggc ctccgccgcc gctgcgaccg tgcggaacgc cccgagcagc
      781 acccgctttt ccggcggtat cggcaggagc cgcacggtaa tctcggtgat caccgcgagc
      841 atgccttccg agcccgacat cagcgcgagc agatcgtaac cgggagagtc taggcgctta
      901 ccgccgatct gcagctcgtc gccttcgacg gtgagcacct tcagccccaa aacgttgtga
      961 acggtgaggc cgtacttcag gcaatgaacg ccgccggagt tttccgccac gttgccgccg
     1021 atgctgcagg cgatctggga ggaagggtcg ggcgcgtagt aaagcccgta agcggatgct
     1081 gcctcgctga tggccaggtt gcggacgcct ggctcgaccc gggccacacc gttttccacg
     1141 tcgatttcga gaattcgatc caaccgggaa acccccagca ccaggccgtc gctgacgggc
     1201 ctcgccccgc cggagagccc ggtcccggcc ccccgcggaa ccaaaggcac ctcgtgctcg
     1261 gcgcagattc gcagcacctc ccgcacctct gcctcggtct gtggaagcgc cacggcccag
     1321 ggcatctgcc tgagcgcggt gagcccgtcc gtttcgtagg gcttgagctg gtgccgatcg
     1381 gtgagaacgg ctccctcggg cagggcggca cgcagctgtc ggagagcgct ggcatggttc
     1441 atggtggagg agtatacgcc atggaagacg gcgtctttca cgtcggcgag cgcatcctgc
     1501 ccgccaaatc ggctaaagta accccaacgc agaaaccgcc cacgggaagc accgcgcggc
     1561 gaacggacca gacttcatga ccaacagcta tctcatcagc aacgcccgga tcgtcaatga
     1621 ggggaccatc acggaaggtg atctcctcgt cgagaacggc cgaatctccg gcatcggcaa
     1681 accggcaccg gcgggcaccg acgttttcga cgccaacggc gcctggctcc tgcccgggat
     1741 gatcgacgac caggtgcact ttcgagagcc cggtttcgag cacaagggaa ccatcgccac
     1801 ggaatcccgc gcggccattg ccggaggcat aacgagctac atggagatgc cgaactgcaa
     1861 cccgctgacg gttactcatg aggcgctggc cgacaagcac gttcgcgcaa aggaccattc
     1921 cgccgcgaat ttcgccttct actttggcgc caccaacgac aacctggagg ccatcaagac
     1981 cgtggatccc gccctcacct gcggaatcaa ggtcttcatg ggggcgagca ccggcaacat
     2041 gctggtggac gacgccgaca ccttagaggg cattttcgcc ggcacgccgc tgatcgtggc
     2101 cacccactgc gaggacacgc ccaccatcct ggccaacgag gaacgcgcca gggaagcgtt
     2161 cggcgacgcc atcccgttct cggagcaccc tcgaataagg tccgaggaag cctgctggct
     2221 gtcatcgtcc atggccgtcg gtctcgcccg cagacacggc acccggctcc acgtgctgca
     2281 cctgaccacg gcgcgcgaga tggcgctttt cgagccggga gacctgatgg gcaagcagat
     2341 cacggccgaa gcctgcgtgc accacctctt tttcaacgag agctggtacc ccgacaaggg
     2401 tgccgacatc aagtgcaacc ctgccatcaa gggcgccgct gaccaggaag ccctcctgcg
     2461 cgcggtggcg gaagaccgca tcgacgtgat cgccaccgat cacgcccccc acacccggga
     2521 ggagaaggat cgcgagtacc tgcgcgcccc ggccgggctg ccgctggtgc agcacgccct
     2581 ttcatcgctg gtggagcagg tccaccgcgg cgtgttcgac atcgagac
//
LOCUS       DBNHOX010000424         2803 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_998041, whole genome shotgun sequence.
ACCESSION   DBNHOX010000424 DBNHOX010000000
VERSION     DBNHOX010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_998041"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="AC16UZ_09395"
     CDS             <1..332
                     /locus_tag="AC16UZ_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HIR6246938.1"
                     /translation="IYANEALFLAGLRPTARAGRVTRLGYDRLVAAIKQVLTEAIDMG
                     GTTLRDFVNQDGNPGYFKQSLNVYGRLGLPCRRCATPLKGTRTGQRATVFCPNCQGAQ
                     SFSGRNR"
     gene            395..706
                     /locus_tag="AC16UZ_09400"
     CDS             395..706
                     /locus_tag="AC16UZ_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246939.1"
                     /translation="MKRMKRAILAFTLALFMATPALAAPEDSPSAFTMTGDLVVARPL
                     GVAMTAIGAAVFVVSLPFSALGGNVGESADALVMGPVNETFVRCLGCPSAGRRQAPPA
                     N"
     gene            complement(755..1228)
                     /gene="coaD"
                     /locus_tag="AC16UZ_09405"
     CDS             complement(755..1228)
                     /gene="coaD"
                     /locus_tag="AC16UZ_09405"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR6246940.1"
                     /translation="MRTVVYPGSFNPITNGHTDLVKRALNLFDHVIVAIGTSAQKDPS
                     VALKNRIDLCRLVLAEFGDKVTVEGFNSLLVNYVRERNARFILRGLRTVTDFDYEFQM
                     AEMNQSLAPEIEYVFLPTAQNCSFISSTLVREIASLGGDISQFVHPAVVEAYHRD"
     gene            complement(1271..1858)
                     /gene="rsmD"
                     /locus_tag="AC16UZ_09410"
     CDS             complement(1271..1858)
                     /gene="rsmD"
                     /locus_tag="AC16UZ_09410"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR6246941.1"
                     /translation="MSDWHGSLTILRMASNQVRIIGGKWRGRKLAFPDAATLRPTLGR
                     VRETLFNWLAGRIAGACCLDLYAGTGALGFEALSRGAAEADMVERARTAVAALRRNAQ
                     LLKAPARVHGIPAERFLQATDRTWDVIFLDPPFADDALPRALEVIAERGLLRPGGCVY
                     FEQAERGGEEPAGPGWRLLKKSRAGASEFGLLVRD"
     gene            1899..>2803
                     /gene="ftsY"
                     /locus_tag="AC16UZ_09415"
     CDS             1899..>2803
                     /gene="ftsY"
                     /locus_tag="AC16UZ_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR6246942.1"
                     /translation="MSEQDQSSAEGGGLWSRLKRGLGQTRNQLAEGVGNLLLGEKEID
                     DEVLSELETALLLTDVGIDTTREIMDKLAVRVRRKELNDTVALHQALGSLLKELLLPV
                     EKPFTIAQDKRPFVIFMVGVNGAGKTTTIGKLAKRLRDDGLNVVLAAGDTFRAAAVEQ
                     LQTWGERIGVPVIAQGQGSDSASVVYDAVAAARARGHDGVLADTAGRLQAQANLMEEL
                     AKVKRVVNRMDPDAPHEVLLVLDAGVGQNALSQVREFDAAGGVTGLIFTKLDGTAKAG
                     VLFGIARQVMKPVYFVGVGEGADDL"
BASE COUNT          505 a          880 c          945 g          473 t
ORIGIN      
        1 acatctatgc caacgaggca ctgttcctgg ccggcctgcg gcctacggca cgggcaggcc
       61 gtgtcaccag gcttggctac gatcggctgg tggcggccat caagcaggtg ctgacggaag
      121 ccatcgacat gggcggcacc acattgagag attttgtcaa ccaggatggc aatccgggtt
      181 atttcaagca gtcgctcaac gtctatggcc ggctcggatt gccctgccgt cgctgcgcga
      241 cgccgctcaa agggacccgc accgggcagc gagctaccgt tttttgtcca aattgccaag
      301 gcgcccaaag ttttagcgga cgcaatcgct aaagtaggcg cggtgcgctc ggcaccagga
      361 aaagggtcga gggcggttga ctagcaaaaa ggacatgaag cgaatgaaac gcgcaattct
      421 ggcattcacc ctggccctgt tcatggccac tccggcactt gctgcgccgg aggactcacc
      481 ctctgcattc acgatgacgg gcgatcttgt cgtcgccagg ccccttggcg tcgccatgac
      541 ggcaatcggc gccgcagtat tcgtcgtcag cttgccgttc tcggcacttg gcggaaacgt
      601 cggcgagtcc gcggacgcgc tggtcatggg ccccgtcaac gaaacgtttg tgcgttgcct
      661 cggttgcccc agcgccggtc gccgccaggc gccgcccgcc aactgatttc cccctgatgc
      721 cggggctctt cgataggggc cccggtgctg gtggctagtc ccggtggtag gcctcgacca
      781 ccgcgggatg gacgaattgg gagatgtctc cgcccaggga ggcgatctcg cgcaccagcg
      841 tggacgaaat gaaagagcag ttctgcgctg tgggcagaaa cacgtactcg atctccggtg
      901 ctagcgactg gttcatctcc gccatctgaa actcgtagtc gaagtcggtc accgtgcgca
      961 ggccgcgcag aatgaaccgc gcgttgcgtt cccgcacgta gttcaccagc agggaattga
     1021 atccttccac ggtgactttg tcgccgaact ccgccagcac caagcggcag agatcgatcc
     1081 ggttcttcag cgccactgaa ggatctttct gtgccgacgt gccgatggcc acgatgacat
     1141 ggtcgaagag gttgagcgcc cgcttgacca gatccgtgtg tccgttagtg atcgggttga
     1201 acgaacccgg gtatacgacc gtgcgcattg tccccacccc actgagaagc ccgcgatggt
     1261 atcggggcgg tcaatccctg acaagcaggc cgaactcgct ggcgccggcg cggctcttct
     1321 tcagcaggcg ccagccaggg ccggcgggct cctcgccccc cctttcggcc tgctcgaagt
     1381 aaacgcagcc gccgggccga agcaggccgc gctcggcgat cacctctagg gcgcggggca
     1441 gcgcatcgtc ggcgaatggc ggatccaaga agatcacgtc ccaggtgcgg tccgtggctt
     1501 gcaggaaacg ttcggctgga atgccgtgaa cgcgggctgg cgccttcagc agctgcgcgt
     1561 tgcgtcgcag cgcggccacg gcggtacgcg ccctctccac catgtctgcc tccgcggcgc
     1621 cgcgggacag cgcttcgaaa cccagcgcgc cggtgccggc gtagagatcg aggcagcagg
     1681 cgccggcgat ccggcccgca agccagttga acagagtttc gcggacgcga cccagggtgg
     1741 gcctgagcgt ggcggcatcc gggaaggcga gctttcgtcc cctccactta ccgccgataa
     1801 tacgcacctg gttgctggcc atccgaagga tggtgagcga accgtgccag tcagacaagc
     1861 cgtagaccgg cgagcgcatc gccgggaaaa ggatcatcgt gagcgagcag gatcagtcga
     1921 gtgcggaggg aggagggctg tggagccggc tgaagcgggg gctcggccag acgcgcaacc
     1981 agctggccga aggcgtcggc aatctgcttc tcggcgagaa ggagatcgac gacgaagttc
     2041 tctcggagct ggagaccgcg ttgctgctca ccgacgtcgg catcgacacc acccgggaga
     2101 tcatggacaa gctcgccgtg cgcgtccgcc gcaaggagct caacgacacc gtcgcccttc
     2161 accaggcatt ggggagtctg ctcaaggagc ttttactgcc cgtcgagaag cccttcacca
     2221 ttgcacagga caaacgtccg tttgtgattt tcatggtggg cgtcaacggc gcgggaaaga
     2281 cgacgaccat cggcaagctg gccaaacgac ttcgcgacga cggcctgaac gtggtgctgg
     2341 ccgcgggcga cacctttcgc gctgcggccg tggagcagct tcagacctgg ggcgagcgga
     2401 ttggcgtgcc ggtcatcgcc cagggtcagg ggtccgacag cgcctcggtg gtctatgacg
     2461 ccgtggcggc ggcccgggct cgcggccacg acggcgtgct ggcggacacc gcggggcgcc
     2521 tgcaggcgca ggccaatctc atggaggaac tcgccaaggt gaagcgggtc gtgaatcgca
     2581 tggacccgga cgctccccac gaggtcctgc tcgtgctcga tgccggcgtg gggcagaacg
     2641 ccctcagcca ggtccgcgag ttcgacgcgg ccggtggggt gacggggctg atcttcacga
     2701 agctggacgg aacggccaag gccggggtgc tgttcgggat cgcgcgtcag gtcatgaagc
     2761 ccgtctactt cgtcggcgtg ggcgagggtg cggacgacct cag
//
LOCUS       DBNHOX010000425         2934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_998107, whole genome shotgun sequence.
ACCESSION   DBNHOX010000425 DBNHOX010000000
VERSION     DBNHOX010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_998107"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="AC16UZ_09420"
     CDS             <1..605
                     /locus_tag="AC16UZ_09420"
                     /inference="COORDINATES: protein motif:HMM:NF016269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HIR6246943.1"
                     /translation="GEPTMRMAIGFATLCIVLTLPSEANSAFGKKKSPEERRENITEM
                     REDVLNRLYDERASAKAQVQDSVGYAVFSNTGVNLFLLSTANGAGVAHDNGAGKDIFM
                     NMFSAGGGIGLGVKKFSAVFIFHTRDAFDQFVEEGWNFTGQADAAATTDAEEDEQVGA
                     AGGTVGINDGVTVYQMTEKGLALQATLQGTKYWKNEDLNN"
     gene            complement(617..1201)
                     /locus_tag="AC16UZ_09425"
     CDS             complement(617..1201)
                     /locus_tag="AC16UZ_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246944.1"
                     /translation="MKVIRLLLGVIVISGANVALGDGHGGDGLVTFSATQKETLTAIV
                     EAIDHETRVVTVRGPRGNAITFTASDEVRNLDQVAAGDLIDAEYVESLDVEVLPGDGS
                     EPGHGVMGAVARTDEGEMPGVAAAEVNVITAKIVAIDLEASTATLEGPDGALEEIEPM
                     NPSNLERVSVGDIVVFTVTQSVALSVREQRADAE"
     gene            1461..1829
                     /locus_tag="AC16UZ_09430"
     CDS             1461..1829
                     /locus_tag="AC16UZ_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246945.1"
                     /translation="MRIPKYLALCALVALGGCASWSQETGVDNRWRGAEVPVWVTGET
                     TAEDVMTALGPPSQIIALKDQVVYYYLKETMAGSAYVLILYNNAKMRAEYDRAIFFFD
                     DGGKLTRYAYSNETLSHRGG"
     gene            1829..2458
                     /locus_tag="AC16UZ_09435"
     CDS             1829..2458
                     /locus_tag="AC16UZ_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246946.1"
                     /translation="MPIVAIATLAILLAASSGCSMIHQDVGKKPPREASRALADRADA
                     TVDDVLEALGPPHAMSAINDGYAFLYHWYAVEERQLGLSSNWPVLRWFRLAIARADID
                     ATALIVHFDDQGGVRAYGLDQRSIDLGGGTGFQPIFVVTPLVDADEYLEDIWPAHRWG
                     MSMLREAPATLNAHAGVDSGMQGIEMRSTSLKVGQRTLEMQKEQSSSSR"
     gene            complement(2469..>2934)
                     /locus_tag="AC16UZ_09440"
     CDS             complement(2469..>2934)
                     /locus_tag="AC16UZ_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246947.1"
                     /translation="DGDTKIYRDDFGSDFEAETYKLALNHYRAMRTRDALALRAVIKA
                     TGGNAPFFLLSSFGSGPDLRGYPAGRYRDKLMYAVQGEYRWHLHDSWILTGFAGVGEV
                     ADEIGDFGDDFLPAAGIGARYVLSKKHRVSISADLAVGKHGAEFYIGIGEAF"
BASE COUNT          566 a          965 c          913 g          490 t
ORIGIN      
        1 gaggggaacc aaccatgcgc atggccatcg gatttgcaac gctctgcatc gtcctgaccc
       61 tgccgtcgga ggccaactcc gccttcggca agaagaagag ccccgaggag cgtcgggaaa
      121 acatcaccga gatgcgcgaa gacgtcctga acaggctcta cgacgaaaga gcatcggcca
      181 aggcacaggt tcaggattcg gtgggttacg ccgtgttctc caacacgggc gtcaatctgt
      241 tcctgctctc cacggccaac ggcgcaggcg tcgcacatga caacggggcc ggcaaagaca
      301 tcttcatgaa catgttctcc gccggtggcg ggatcggtct cggcgtcaag aagttcagcg
      361 ccgtgttcat ttttcacact cgcgacgcct tcgatcagtt cgttgaggag ggctggaact
      421 tcaccggcca ggcggatgcc gcggcgacca ctgacgccga ggaagacgag caggtcggcg
      481 ccgccggcgg caccgtcggg atcaacgacg gcgtcaccgt gtaccagatg accgagaaag
      541 gcctcgcgtt acaggcgacc cttcagggca ccaagtactg gaagaacgaa gacctcaaca
      601 actgacggtt gaacgcctac tcggcgtcag cccgctgctc gcgtaccgag agcgcaacgg
      661 actgggtgac cgtgaagacc acgatgtcac ccaccgagac ccgctccaga ttggacgggt
      721 tcatcggctc gatctcctct agcgccccat cgggaccttc gagggtagcg gtgctggcct
      781 cgaggtcaat ggcaacgatt ttcgccgtga tcacgttgac ctcggctgcc gccacgccgg
      841 gcatctcgcc ctcatcggtc cgagcaacgg cgcccatcac gccgtgccct ggctcgctgc
      901 catctcccgg cagcacctcc acgtccagag actccacgta ctccgcgtcg atgagatccc
      961 ccgccgccac ctgatcgaga ttgcggacct cgtctgacgc ggtgaaggtg atggcgttgc
     1021 cccggggtcc cctgacagtg accacccgag tttcgtggtc gatggcctcc acaatcgccg
     1081 tgagcgtttc cttctgggtg gccgagaacg taaccaggcc atcgcccccg tgcccgtcgc
     1141 caagtgcgac gttagccccc gagatgacga taacccccag caacagccga ataaccttca
     1201 tggcagtgga ccccttgaat tgagcgcggc tgatgatatc ggcgacggac attcacgcgc
     1261 aacgcggacg gcgcggcgaa agcgaggagg ggcgagcacg agtgactcca gcgcttccgg
     1321 tcacccctcc ggtcactatc gaatccgagt ggaggccgct atcccctcct gctatggtct
     1381 ggaaaccaat tgcgccgggc cggcaggata agatcgacaa gcgcgctgcc tgcgccggta
     1441 tggcggaacc agaggacatc atgagaattc caaagtatct ggcgctgtgc gcccttgtgg
     1501 ctctgggcgg ctgcgcgagc tggagtcagg agaccggtgt ggacaaccgc tggcgcggag
     1561 ccgaggtgcc cgtctgggtc accggcgaaa ccacggcaga ggacgtcatg accgcactcg
     1621 ggccgccttc ccagatcatc gccctcaagg atcaggtggt ctactactac ctgaaggaga
     1681 caatggccgg ctcggcctat gtgctgattc tctacaacaa cgcgaagatg agggccgagt
     1741 acgatcgggc catcttcttc ttcgacgacg ggggcaagct aacccgttac gcgtacagca
     1801 acgagacgct ttcccaccgg ggcggctgat gcccatcgtc gccatcgcga ccctcgcgat
     1861 cctgctcgcc gcatcgagcg gctgctcgat gattcaccag gacgtcggca agaagccgcc
     1921 acgcgaggcg tcccgagcgc tggccgaccg tgcggacgcc accgtggacg atgtgctgga
     1981 ggcgctgggc ccaccgcacg ccatgagcgc gatcaacgac ggttacgcct tcctgtacca
     2041 ctggtacgcg gttgaagaac gccagctcgg gctctcgtcc aactggcccg tgttgcgctg
     2101 gttccggctg gccatcgccc gagccgacat tgacgccacc gcgttgatcg tgcacttcga
     2161 cgaccagggt ggcgtccggg cctatggcct ggatcagcga tccatcgatc tgggcggggg
     2221 cacgggcttc cagccaattt tcgtggtgac gcccctggtg gacgcggacg agtatctcga
     2281 ggacatctgg cccgcccacc gctggggcat gtccatgctg cgcgaagccc ccgccacgct
     2341 gaacgcccat gcgggcgtgg attccggcat gcaggggatt gaaatgcgct cgacgtcgct
     2401 gaaggtgggg cagcgcaccc tggagatgca gaaagagcaa agcagctcca gccgctgaac
     2461 ggccgcggtc agaacgcctc gccgatgccg atgtagaact cggcgccgtg cttgccgacg
     2521 gccaggtccg cagaaatact cacccggtgc ttcttcgaca gcacgtagcg cgcaccaatg
     2581 cccgccgccg gcaggaagtc gtcgccgaag tcgccgatct cgtcagccac ctcacccacc
     2641 ccggcaaagc ccgtcaggat ccagctgtcg tgaaggtgcc agcggtactc accctgcacg
     2701 gcgtacatga gcttgtcacg gtagcgtccg gccgggtaac cgcgcagatc ggggccgctg
     2761 ccgaagctgc tgagcagaaa gaacggcgcg ttaccaccgg tggccttgat caccgcacgc
     2821 agggccagag cgtcccgggt gcgcatggcc cgatagtgat tcagagcgag cttgtaggtc
     2881 tccgcttcga aatcgctgcc aaagtcgtcg cggtagatct tggtgtcccc atcg
//
LOCUS       DBNHOX010000426         3996 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1447520, whole genome shotgun sequence.
ACCESSION   DBNHOX010000426 DBNHOX010000000
VERSION     DBNHOX010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1447520"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="AC16UZ_09445"
     CDS             complement(<1..770)
                     /locus_tag="AC16UZ_09445"
                     /inference="COORDINATES: protein motif:HMM:NF039989.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR6246948.1"
                     /translation="MPHIVDTLIEERARGLMQHPLLWRLVTGLLYPVLGYRAAVRMAD
                     TVADMGGVEIFEMLSAQLKLDIDLGGTEHIPETGSAVVTANHPTGIADGVAVFDVLRT
                     VRSDITFFANRDAIRFAPGLADVIVPVEWVEDKRTHARTRETVSHMVRAFRSERLIVI
                     FPSGRLARPTLRGLVERGWTPTAVNLAQKYRCPIVPMHIRGRNSWLYYLLYAINTELK
                     DMTLFRELLNKTGQPYRIRIAEPFDPVGDARMLTIALR"
     gene            complement(758..1534)
                     /locus_tag="AC16UZ_09450"
     CDS             complement(758..1534)
                     /locus_tag="AC16UZ_09450"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6246949.1"
                     /translation="MHLDWQIHDGKGPHLLLVHGFLCSRAQWLWNLTVLAEVCRPVTV
                     ELWGHGRSPAPSDPACYAPDRIVEEFESIRTALGAEQWFVLGYSLGAGLTIRYALTHP
                     HRVMGHVFTNSTSGLADAEQIRRWQDEADETAQRIIAAGQAALERIPVHPRHATKLPD
                     PLHQALMADAARLDPTGVANVLRHTNPNVSVRADVHRNTRPALLVCGSRERRFQPHRA
                     FAQATMPCLEIVDIPAGHGVNMEAHEPFNAAVSSFVRCHT"
     gene            1650..3341
                     /locus_tag="AC16UZ_09455"
     CDS             1650..3341
                     /locus_tag="AC16UZ_09455"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6246950.1"
                     /translation="MAYDILIRNGTVVDGSGGAPYPADVGIRNGRIASIGRIREKART
                     TIDAEGHVVSPGFVDGHTHMDAQVFWDPIGSSSCYHGVTSVVMGNCGFTLAPCRRDEA
                     DLVFRNLERAEDLSRDAMLTGIDWQWETFPQFLDAVDGLPKGINYAGYIGHSALRTYV
                     MGERAFTDPATDGDLAAMCRQVQEAVRAGAIGFSTSRTFNHITADDRPVASRLAEWDE
                     VRAIVNAMGETGKGIFEIAGEAPGRDPARIRDYHERLRDLAVESGVTQTWGMFSVRIA
                     PDFWRPYFDLLDETAAAGGRMYAQVHSRALNVLLSFKTSTPFDKWDVWNDVRSLPLDE
                     QKAKLRDPALRARLVEAANREYQGPRVVGAEARPPNWEWVYPMADMSYDQPSMAELAA
                     ERGVDPVELMIDMSLAHDFDIFFRQPIANENQDDVLEMMKHPRAMVTFSDSGAHVSQI
                     MDSSLQTHLLSYWVREKQAFTLPEAVRRITYDTATMWGLHDRGLVRAGMAADLVIFDP
                     ETIGPRMPTVEHDLPAGARRLKQTADGIMHTIVNGEVLLTRNEYGGATPGRLLRA"
     gene            complement(3389..3465)
                     /locus_tag="AC16UZ_09460"
     tRNA            complement(3389..3465)
                     /locus_tag="AC16UZ_09460"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3429..3431),aa:Arg,seq:tcg)
     gene            3590..>3996
                     /locus_tag="AC16UZ_09465"
     CDS             3590..>3996
                     /locus_tag="AC16UZ_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR6246951.1"
                     /translation="MNELPLVGVTVLDFGQVYNGPYCGFLLAQAGARVIKVESPRGET
                     LRSRGETTGASYPFAMLNGNKECISLNFRSARGQALLRDLVMHVDVVLENFAPGTLER
                     YGVGARTLRGLNPELIYASSTGYGRSGAHRDYRG"
BASE COUNT          675 a         1270 c         1390 g          661 t
ORIGIN      
        1 tcacgcagcg cgatggtcaa catcctcgca tcgcccacgg ggtcgaacgg ctcggcgatg
       61 cggatccggt acggctggcc ggttttgttg agcagctcgc gaaacagcgt catgtccttc
      121 agctcggtgt tgatggcgta cagcagatag tagagccagg agttcctgcc gcggatgtgc
      181 atggggacga tggggcatcg gtacttctgc gccaggttca ccgccgtagg cgtccacccg
      241 cgctccacca ggcctcggag agtgggccgg gccaggcgcc cggaaggaaa tatgacgatc
      301 agcctctcgg accgaaacgc gcggaccatg tggctgacgg tctcccgggt ccgtgcgtgc
      361 gttcgcttgt cttccaccca ctcgacgggc acgatcacgt cggcgaggcc cggcgcgaag
      421 cgaatggcat cccgattggc gaagaacgtg atgtccgacc tcaccgtgcg cagcacgtcg
      481 aacacggcga cgccgtcggc gatgccggtg gggtgatttg ctgtgaccac cgcgctgccc
      541 gtttcgggga tgtgctcggt gccgccaagg tcgatgtcga gcttcagctg cgcgctgagc
      601 atctcgaaga tctccacccc gcccatgtcg gccaccgtat cggccatgcg gaccgccgcg
      661 cgatacccca gcacgggata cagcaggccg gtcaccagcc gccacagcag ggggtgctgc
      721 atcagtcccc gggcccgttc ctcgatgagc gtgtcgacta tgtgtggcat cggacgaacg
      781 agctgaccgc cgcgttgaac ggttcgtggg cttccatgtt gacgccgtgg ccggctggaa
      841 tgtcgacgat ctcgaggcag ggcatggtcg cctgggcgaa cgcgcgatgg ggctgaaagc
      901 gccgttctct cgagccgcag acgagcagag cgggacgggt gttgcgatgc acgtccgcgc
      961 gcacggacac gttcgggttg gtgtggcgta gaacgttcgc gacgcccgtc gggtcgagac
     1021 gtgcggcgtc cgccatgagg gcctggtgca gaggatcagg gagcttggtc gcgtgacggg
     1081 gatgtacggg gatgcgttcc agggcggcct gacctgcggc gatgatccgc tgcgcggtct
     1141 cgtccgcctc gtcctgccag cgccgtatct gctcggcgtc cgccaggccg gaggtcgagt
     1201 tggtgaagac gtgtcccatg acccgatggg gatgggtcag cgcgtagcgg atggtcaggc
     1261 cggcaccgag ggagtagccg agcacgaacc attgctcggc gccgagggcc gtgcggatgg
     1321 actcgaactc ttccacgatc cgatccggcg cgtagcacgc cggatcggac ggtgccggcg
     1381 atcgaccgtg gccccagagc tccacggtga cggggcggca gacctcggcc aggacggtca
     1441 ggttccacag ccattgggcg cggctgcaca gaaagccgtg gaccagcagc aggtgcggtc
     1501 ccttgccatc gtgtatctgc cagtcgagat gcatgtaaac ggctgccctt gggatgacgc
     1561 aaaataagac attaaccgat ggcccgccat acggacggtg tcgctccgtc gcggacgccg
     1621 gtgatagact gccacgcaag gagggcggca tggcttacga tattctgatc agaaacggaa
     1681 cggtggtgga tggttccggt ggggcgcctt acccggccga cgtgggcatt cgcaacgggc
     1741 gtatcgcgtc catcggcagg atccgggaaa aggcccggac taccatcgat gccgaggggc
     1801 acgtggtcag cccgggattc gtggacggcc atacgcacat ggacgcccag gtgttctggg
     1861 atcccatcgg gtccagctcc tgctatcacg gcgtgacgag cgtggtcatg ggcaactgcg
     1921 gcttcaccct ggcaccgtgc cggcgggacg aggcggacct ggtcttccgc aatctcgaac
     1981 gtgccgaaga tctctcccgg gacgccatgc tcacggggat cgactggcag tgggagacct
     2041 ttccgcagtt tctcgatgcg gtggacggcc tgcccaaggg gatcaactac gccggctaca
     2101 tcggtcattc ggcccttcgg acctacgtca tgggcgagcg cgctttcacc gacccggcca
     2161 ccgacgggga tctggcggcc atgtgccggc aggtgcagga agccgtgcgc gcgggcgcca
     2221 tcggcttctc gacgtcgcgc acgttcaacc acatcaccgc cgacgaccgt cccgtggcca
     2281 gccgtctggc agagtgggac gaggtgcgcg ccatcgtcaa cgccatgggc gagacgggca
     2341 aaggcatctt cgagatcgcc ggcgaagcgc cgggccgtga tccggctcga attcgcgact
     2401 accacgaacg gcttcgcgac ctggccgtgg agtccggggt gacccagacc tggggcatgt
     2461 tcagcgtgcg catcgcgccc gatttctggc gaccctattt cgatctcttg gacgagaccg
     2521 cggctgcggg agggcgaatg tacgcccagg tgcacagccg ggcgctcaac gtgctgctgt
     2581 cgttcaagac ctcgacgccg ttcgacaagt gggacgtctg gaatgacgtc cgctcgctcc
     2641 ccctggacga gcagaaggcg aagcttcgcg atcccgccct gcgcgcccgg ctggtcgagg
     2701 ccgccaatcg cgagtaccag ggccctcgtg tggtgggcgc cgaagccagg ccgccgaact
     2761 gggagtgggt ctaccccatg gcggacatga gctacgatca gcccagcatg gccgagctgg
     2821 ccgccgagcg cggcgtggat cccgtggagc tcatgatcga catgtcgctg gcgcacgact
     2881 tcgacatctt cttccgtcag cccatcgcca acgaaaatca ggacgacgtg ctggaaatga
     2941 tgaagcatcc gcgggcgatg gtgaccttct ccgattccgg cgcccacgtc tctcagatca
     3001 tggacagctc cctgcagacc cacctcttga gctactgggt gcgggagaag caggcgttca
     3061 ccctgcccga ggcggtccgg aggatcacct acgatacggc caccatgtgg ggcctgcacg
     3121 atcgcggtct ggttcgggca ggcatggcgg cggacctggt gattttcgac cccgaaacca
     3181 tcggcccgcg gatgcccacc gtggagcacg acctgccggc gggcgctcgc cggctcaagc
     3241 agacggccga cgggatcatg cacaccatcg tcaacggcga ggtgctgctg acccgcaacg
     3301 agtacggcgg agcgaccccg ggcaggctgc tgcgcgcctg agctcgcacg ggcaggcggc
     3361 cgctcggggc tgccaggaag aagggatctg gcgcggctgg gaggactcga acccccgaca
     3421 cctgggttcg aagcccagtg ctctatccag ctgagctaca gccgctctgg aagagtcggt
     3481 cagtataacc gtatccacct gggcgggcgc tgccttttcc cgtgagcagt ggtggctggc
     3541 cagcaaaccg gctaccgttc gtgcagagac gatgctggtg gggtgcgcga tgaacgagct
     3601 gccgctggtc ggtgtgaccg tgctggattt cggccaggtc tacaacggac cgtactgcgg
     3661 gttcctgctt gcccaggcgg gcgcgcgggt catcaaggtc gagtcgccgc ggggcgagac
     3721 cctgcgcagc cggggcgaga cgaccggcgc gagctacccg ttcgccatgc tcaacgggaa
     3781 caaggaatgc atctcgctga acttccgctc cgctcgcggc caggcgctgc tgcgggacct
     3841 ggtcatgcac gtcgacgtgg tgctggagaa tttcgcgccc ggtacgctgg agcgctacgg
     3901 ggtgggggcc agaaccctgc gtggcctcaa ccccgagctc atctacgcgt cgagcacggg
     3961 ctacggccgg tcgggcgcgc accgcgacta ccgcgg
//
LOCUS       DBNHOX010000427         2006 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1190716, whole genome shotgun sequence.
ACCESSION   DBNHOX010000427 DBNHOX010000000
VERSION     DBNHOX010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1190716"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="AC16UZ_09470"
     CDS             complement(<1..453)
                     /locus_tag="AC16UZ_09470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019750117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR6246952.1"
                     /translation="MIIGAGTAGCVLANRLSADKDADVLLLEAGGKDDYFWIDIPVGY
                     LYTINNPRTDWCYQVEPEPGLNGRRINYARGKVLGGCSSINAMIYMRGQQSDYDYWAS
                     LGNRGWSWRDVLPVFMRSEDYQHGASDLHGVGGELRVEERRVNWEILDA"
     gene            complement(476..1873)
                     /locus_tag="AC16UZ_09475"
     CDS             complement(476..1873)
                     /locus_tag="AC16UZ_09475"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6246953.1"
                     /translation="MDYDLTKPADPTLAPGDARCPGPSTRDIILKEQDGAPAALTAES
                     WSFLGDEDIPFSRYTSQPFYDREIETIWRKTWQWACREEHIPNPGDWTVYDVGPYSVV
                     VIRDEGGAVKAYVNSCPHRGMQFCDAGSSGRGKQFLRCPFHGMSWHLDGSLREVPCRW
                     DFPHIEDENFGLTEVPTGTWAGFVFINLDPDCEPLESYLEVLPEHFKNWKLEDRFVAL
                     HTRKILPGNWKMCMEAFLEAYHVLATHPEGIHSAGDANADYDIFGRHVTRFVHTIGYP
                     SPHVASEVDERKLFAALGHNPDDLPDGMSARQRHADIMREQLGREWDVDLSGLSNSEM
                     LDSIEYFVFPNACFFPGINIRLVYRFRPIDVDHTMHEILLLQPVPEHGERPKPAEPID
                     LTIDESYEKANEVSLGGFALAMVLDQDTENFHRQRAGIKAAQRAGVRTAAVLANYQES
                     RIRRLHMTVDEYMAG"
BASE COUNT          327 a          618 c          683 g          378 t
ORIGIN      
        1 ggcgtcgagg atttcccagt tcacgcggcg ctcctccacg cgcagctcgc cgcccacgcc
       61 atgtaaatcg ctcgcgccgt gttggtaatc ctcggagcgc atgaacaccg gcagcacgtc
      121 ccgccagctc cagccacgat tgcccaggct cgcccagtaa tcgtaatcgc tctgctggcc
      181 gcgcatgtag atcatggcgt tgatggacga gcagccgccg aggaccttgc cgcgggcgta
      241 gttgatgcgg cggccgttga ggccgggctc gggctccacc tgatagcacc agtccgtgcg
      301 cgggttgttg atggtgtaca ggtagccgac cgggatgtcg atccagaagt agtcgtcctt
      361 gccgccggct tccagcagca ggacgtcggc atctttgtca gcgctcagac ggttcgccag
      421 cacgcagccg gcggtgcccg ctccgatgat gatgtagtcg aactcgcgaa cctgactaac
      481 ccgccatgta ctcgtccacc gtcatgtgca gccggcggat ccgggactcc tggtaattgg
      541 ccaggacggc ggccgttcgc acacccgccc tctgtgcggc cttgatgccc gcgcgctgac
      601 ggtgaaagtt ctccgtgtcc tggtccaaga ccatggccag cgcaaacccg ccgagggaga
      661 cctcgttggc cttctcgtat gactcgtcga tggtcaggtc gatgggctcg gccggcttgg
      721 gccgctcgcc gtgctccggc acgggctgca gcagcaggat ctcgtgcatg gtgtggtcca
      781 cgtcgatggg ccggaagcgg tacaccaggc ggatgttgat gccggggaag aagcaggcgt
      841 tggggaagac gaagtactcg atggaatcca gcatctcgct gttggagagc ccgctcagat
      901 ccacgtccca ctcgcgccca agctgctcac gcatgatgtc cgcgtggcgc tgccgggcgc
      961 tcatgccatc gggcaggtcg tccgggttgt ggccgagggc cgcgaacagc ttgcgctcgt
     1021 ccacctccga ggcgacgtgg ggggacgggt agccgatggt gtgcacgaag cgggtgacgt
     1081 gacggccgaa gatgtcgtag tcggcgttgg cgtcgccggc gctgtgaatg ccttcgggat
     1141 gagtggcgag cacgtggtag gcctccagga acgcctccat gcacatcttc cagttgcccg
     1201 gcaggatctt gcgggtgtgc agggccacga agcggtcctc gagcttccag ttcttgaagt
     1261 gctcgggaag aacttccagg tagctttcca gcggctcgca gtcgggatcg aggttgatga
     1321 agacgaagcc ggcccacgtg cctgtgggca cctcggtcag gccgaagttc tcgtcttcga
     1381 tgtgggggaa gtcccagcgg cagggaacct ccctgagcga gccgtccagg tgccacgaca
     1441 tgccgtgaaa gggacagcgc aggaactgct tgccgcgccc ggatgagccc gcgtcgcaga
     1501 actgcatgcc ccggtgcggg caggaattga cgtaggcttt gaccgcgccg ccctcgtcgc
     1561 ggatgacgac caccgagtat ggtcctacgt cgtaaacggt ccagtcaccc gggttgggga
     1621 tgtgctcctc gcggcaagcc cactgccagg tcttgcgcca gatggtttcg atttcccggt
     1681 cgtagaacgg ctgagacgtg tagcgcgaga acggaatgtc ctcgtctccg aggaacgacc
     1741 agctttcggc cgtgagcgcg gcgggcgccc cgtcctgctc cttcaagatg atgtcgcggg
     1801 tgctcggtcc gggacagcgc gcgtctcccg gagccaacgt cgggtcggct ggtttggtga
     1861 ggtcgtagtc catcttggta ttccccgggt aggtgctgca ctgatgatac atggttcggc
     1921 cagcgagcca accggagcgc ggtgacgtca tcgggttacc tggcgggctc cgcgcgagcc
     1981 gtattccttg tgaccagatc ggggta
//
LOCUS       DBNHOX010000428         1625 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1190777, whole genome shotgun sequence.
ACCESSION   DBNHOX010000428 DBNHOX010000000
VERSION     DBNHOX010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1190777"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /gene="ligA"
                     /locus_tag="AC16UZ_09480"
     CDS             complement(<1..1165)
                     /gene="ligA"
                     /locus_tag="AC16UZ_09480"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR6246954.1"
                     /translation="MTITRAGAGSRISELRRLVRHHNHRYYVLDSPEIADAEYDALYD
                     ELVALEGDHPDLVTDDSPTQRVGGAPLEQFNSVTHEVAMLSLDKCTTHEELADWIERC
                     RGRLEEGEALDFVCEPKVDGVAVALTYENGRLVLGATRGDGQTGEDITANVRTVGAIP
                     LVMRDDAGHDFPPRIEVRGEIYMPVADFEAFNEQARQRDEKTLVNPRNGAAGSLRQLD
                     PKVSASRPLTMYCYSIGWVEGDWQPRTHMEALERLADWGFRVNPDARAVSGLAGCLRY
                     VEEMLERRDSLGYDIDGVVIKVNDLDQQRRLGAVTRKPRWSIAFKYPAEEATTTLIGV
                     EFQVGRTGAITPVARLAPVFVGGVTVSNATLHNMDEVRRLDIRVGDTVMVRRAG"
     gene            complement(1162..>1625)
                     /gene="zipA"
                     /locus_tag="AC16UZ_09485"
     CDS             complement(1162..>1625)
                     /gene="zipA"
                     /locus_tag="AC16UZ_09485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HIR6246955.1"
                     /translation="ETAPEPALEQPPVEELVVMNVLAPRGQPFAGDELFASLRGRGLK
                     FGNMNIFHRLDAGSKKVQFSVANIVEPGTFEMSDMETIRSPGLCFFLQLPGPENPLEA
                     FETMLSVAHGVAEELNGELKDEQRSFMTPQTEEHYRQRILEFSRRRMSKRA"
BASE COUNT          268 a          558 c          503 g          296 t
ORIGIN      
        1 cgcccgcccg cctcaccatg acggtgtcgc ccacgcggat gtccaggcgc cggacctcgt
       61 ccatgttgtg gagcgtcgcg ttgctcaccg tcacgccgcc cacgaagacg ggcgccagac
      121 gagccaccgg ggtaatggct ccggtacgac ccacctgaaa ctccacgcca atcagggtcg
      181 tggtcgcttc ttccgccggg tacttgaagg cgatggacca tcgcggcttg cgggtgacgg
      241 cccccaggcg ccgctgctga tcgaggtcgt tcaccttgat cacgacacca tcgatgtcgt
      301 agcccagcga gtcgcggcgc tccagcattt cctccacgta ccgcaggcat ccggcgagcc
      361 cgctcaccgc acgggcgtcg ggattgacac gaaaacccca gtcggcgaga cgctccaggg
      421 cttccatgtg cgtccggggc tgccagtctc cttccaccca gccgatgctg tagcagtaca
      481 tggtcaacgg gcggcttgcg ctcaccttcg gatccagctg gcgaaggctg cccgcggctc
      541 cgttgcgcgg gttgaccagc gtcttctcgt cgcgctgacg cgcttgctcg ttgaacgcct
      601 cgaaatcggc gacgggcatg tagatctcgc cgcgcacctc gatgcgcggc ggaaagtcgt
      661 gccccgcatc gtcccgcatt accaggggaa tcgcgccgac ggtgcgaacg ttggcggtga
      721 tgtcctctcc ggtctgcccg tccccccggg tggctccgag aaccagccga ccgttctcgt
      781 aggtcagggc gaccgcaacg ccatccacct tgggctcgca gacgaagtcg agcgcttctc
      841 cctcttccag acgtccgcga caacgctcga tccagtccgc gagctcctcg tgggtcgtgc
      901 acttgtccag ggagagcatg gccacctcgt gggtgaccga gttgaactgc tcgagcggcg
      961 cccccccgac ccgctgggtg ggcgaatcgt cggtcacgag atcgggatga tcgccctcca
     1021 gagcaacgag ctcgtcgtac aacgcgtcgt actccgcgtc ggcgatctct ggagaatcga
     1081 gcacgtagta acgatggtta tggtggcgta ccagccgacg gagctcgctg atgcggctcc
     1141 cggctccggc cctggtgatg gtcatgcccg cttggacatg cgccgccgcg agaactccag
     1201 gatccgctgg cggtagtgct cctcggtctg gggcgtcatg aagctgcgct gctcgtcttt
     1261 cagctcgccg ttgagctctt cggcgacgcc gtgggcaaca gacagcatgg tctcaaacgc
     1321 ctcgagcgga ttttcaggtc ccggcagctg cagaaagaag cagagtcccg gcgagcggat
     1381 ggtttccatg tccgacatct cgaacgtccc cggctcgacg atgttcgcca cgctgaactg
     1441 aactttcttg gagcccgcat ccaaacgatg aaagatgttc atgttgccga acttcaggcc
     1501 gcgtcctcgc agcgacgcga agagctcgtc gcccgcaaac ggctgcccac ggggcgcgag
     1561 cacgttcatc accacgagtt cttccaccgg cggctgctcg agcgcaggct caggagccgt
     1621 ttcct
//
LOCUS       DBNHOX010000429         2799 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1127175, whole genome shotgun sequence.
ACCESSION   DBNHOX010000429 DBNHOX010000000
VERSION     DBNHOX010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1127175"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="AC16UZ_09490"
     CDS             <1..730
                     /locus_tag="AC16UZ_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR6246956.1"
                     /translation="QPIETLFVVDAMTGQDAANTAKAFNDALALTGVVLAKADGDARG
                     GAALSVRAITGKPIKFIGMGERVDALEPFYPDRMASRILGMGDVLSLIEEAERKVDKR
                     KAQKLAKKIQRGRKFDLEDFREQLDQMSNMGGVSSMLEKLPGMGQLPPAAQAQLDDGM
                     FRKMAVIIDSMTPHERRFPDVITGSRKQRVARGSGTQVPDVNRLLKQHKQMQRVMKKM
                     SRKGGMQRMMRGMQGMRPPPGVRR"
     gene            829..1101
                     /gene="rpsP"
                     /locus_tag="AC16UZ_09495"
     CDS             829..1101
                     /gene="rpsP"
                     /locus_tag="AC16UZ_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR6246957.1"
                     /translation="MLTIRLARHGSKKNPFYHITVADRTAKRDGRFVERVGFYNPVAK
                     GQAEELRVDLERIDYWLSVGAIASDPVTRLIRRARRQEAAVEATAD"
     gene            1158..1661
                     /gene="rimM"
                     /locus_tag="AC16UZ_09500"
     CDS             1158..1661
                     /gene="rimM"
                     /locus_tag="AC16UZ_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR6246958.1"
                     /translation="MHSQEYVIVGRFGGPYGVHGWIRVTSFTDPPANLLLYGPWHVER
                     DGQWHAYHPVQVRPHQQGLIACYADIPDRSVAAALTGRFIAVPRDCLPVLDAEEYYWR
                     DLEGLEVWNRGARLGVVDHLIDTGANSVLAVRGDSTQTLIPFVSRFVTGVDLAAGRID
                     VDWEEVD"
     gene            1664..2437
                     /gene="trmD"
                     /locus_tag="AC16UZ_09505"
     CDS             1664..2437
                     /gene="trmD"
                     /locus_tag="AC16UZ_09505"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR6246959.1"
                     /translation="MWCGIVSLFPEMFRRLSELGVLGRAIDRGIVTLELFNPREFARD
                     RHRTVDDRPYGGGPGMVMQVEPVVAAVSAAREAARAALGESVPVIYLSPQGRRLTQQR
                     VSELSQQRGVILLAGRYEGIDERAVSLAVDEEVSIGDYVLSGGELPALVVLDAVTRLL
                     PGTLGNSASIEAESHLDGLLDYPHYTRPENTHGLGVPEVLLSGDHEAVSRWWRKMALL
                     RTWQRRPDLLVGRQLDDEDSALLRECFEELAARTESGEE"
     gene            2444..2794
                     /gene="rplS"
                     /locus_tag="AC16UZ_09510"
     CDS             2444..2794
                     /gene="rplS"
                     /locus_tag="AC16UZ_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010220244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR6246960.1"
                     /translation="MRRNKVIEELESEQLKSDLPEFGPGDTVVVQVKVREGNRERLQA
                     FEGVVIGKRNRGLNSAFTVRKMSHGVGVERTFQSHSPMIASVSVKRRGDVRQAKLYYL
                     RERSGRSARIKEKV"
BASE COUNT          500 a          850 c          973 g          476 t
ORIGIN      
        1 ccagcccatc gagacgcttt tcgtcgttga cgccatgacc ggtcaggacg cggccaacac
       61 ggccaaggct ttcaacgatg cgttggcgct aaccggcgtc gtgctggcaa aggccgacgg
      121 ggatgcccgg ggcggtgccg ccctgtcggt ccgcgccatc acgggcaagc ccatcaagtt
      181 catcggcatg ggcgaacggg tcgatgccct ggagcctttc tatccggacc gcatggcatc
      241 gcgaatcctc ggcatgggcg atgtcctcag cctcatcgag gaagccgagc gcaaagtcga
      301 caagcgcaag gcacagaaac tcgccaagaa gattcagcgt ggtcgcaagt tcgacctgga
      361 ggacttccgc gagcagctcg atcagatgtc caatatggga ggcgtttcga gcatgctgga
      421 gaagctgccg ggcatggggc agctgccgcc cgcggcgcag gctcagctgg acgacgggat
      481 gtttcgcaag atggcggtga tcatcgactc catgaccccc cacgaacgcc gcttcccgga
      541 cgtgatcacc ggatcccgca agcagcgggt cgcgcggggc agcggcactc aggtgccgga
      601 tgtgaaccgc ctgctcaaac agcacaagca gatgcagcgg gtgatgaaga agatgtcgcg
      661 caaaggcggc atgcagcgaa tgatgcgggg catgcagggg atgcggccgc cgcccggcgt
      721 gcgtcggtga tggagcctca ccggccttgc atgagccggg cgttccatta aaataccgcg
      781 cctttcaacg attgggcccg cctggcgggc ccgcagcagg gtcatcacat gctaacgatt
      841 cgcctggcac gccacggatc caagaagaac cccttctatc acatcacggt cgcggatcgc
      901 acggcgaagc gagacggcag attcgtcgag cgggtcggct tctacaatcc ggtggccaag
      961 ggtcaggccg aggaactgcg cgttgatctc gagcggatcg actactggct gtccgttggc
     1021 gcgatcgcgt ctgatccggt cacccggctg attcgtcggg cacggcggca ggaagccgcc
     1081 gtggaagcca ccgccgactg acgggccgcg tcgggcgccc cacggcgggc gttgccgggg
     1141 atcccggagt cttccgcatg cattcccagg aatacgtcat cgtcggccgc ttcggcggtc
     1201 cttacggtgt gcacggctgg atccgggtca cctcgttcac ggatccgccc gccaaccttc
     1261 tcctctacgg gccctggcac gtggagcgtg acgggcaatg gcacgcctac cacccggtcc
     1321 aagtgcggcc ccaccagcag ggcttgatcg cctgctacgc agacatcccg gaccgcagcg
     1381 tggcggccgc gctcaccggg cggttcatcg ccgtaccccg ggactgtctg ccggtgctgg
     1441 acgcggaaga gtattactgg cgagacctgg aaggtctcga ggtatggaac cgcggggcgc
     1501 ggctcggcgt ggtggatcat ctcatcgaca ctggcgcgaa ctcggtgctg gcggttcgag
     1561 gcgactcgac gcagacgctg attcctttcg tcagccggtt tgtaaccggg gtcgatcttg
     1621 ccgcgggcag gatcgacgtc gactgggaag aggtcgacta gacgtgtggt gcggcatcgt
     1681 cagtcttttt ccggagatgt tccgacggct ctccgagctg ggcgtgctcg gcagagcgat
     1741 cgatcgaggc atcgtgaccc tcgagctctt caacccgcgc gaattcgccc gggaccgaca
     1801 ccgcacggtg gatgaccggc catacggcgg cgggcccggg atggtcatgc aggtagagcc
     1861 ggtcgttgcg gccgtcagcg cggcgcggga ggcggcccga gccgccctgg gtgagagcgt
     1921 gccggtgatc tacctgagcc cccagggtcg gcggctgacg cagcagagag tcagtgaatt
     1981 gtctcagcag cgcggggtga tactgctggc gggtcgttac gagggcatcg acgagagggc
     2041 cgtctcgctg gcggtggacg aggaagtttc catcggcgac tacgttctca gcggcggcga
     2101 gttgcccgcg ctggtcgtgc tggacgccgt cacgcggctg ctgccgggga ctctcggcaa
     2161 ttcggcctca atagaggctg aatcacacct tgatggattg ctcgattacc ctcactatac
     2221 gcgccccgag aacacccacg gcctgggtgt gcccgaggtc ctgctcagcg gagaccacga
     2281 ggcggtaagc cgctggtggc ggaagatggc gttgctgcgg acctggcagc ggcgccccga
     2341 tctgctggtc gggcggcagc tggatgatga ggacagcgcg ctcttgcgcg agtgttttga
     2401 agaattggcc gctcggaccg agtcgggcga ggagtagaag atcgtgcgca gaaacaaagt
     2461 catcgaggag ctcgagtcgg agcagctgaa gtccgacctg ccggagttcg ggccgggaga
     2521 cacggtcgtc gtgcaggtca aggttcgtga gggtaaccga gaacggctgc aggccttcga
     2581 aggcgtggtg atcggcaagc gcaatcgcgg gctgaactcg gcattcacgg ttcgcaagat
     2641 gtcccatggg gtcggtgtcg agcgcacgtt tcagagtcac agcccgatga tcgcttcggt
     2701 cagtgtcaaa cgtcgcggcg acgtccgtca ggccaagctc tactacctgc gtgagcgctc
     2761 gggccggtct gcgcgcatca aggaaaaggt ctaacgcgt
//
LOCUS       DBNHOX010000430         3012 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1062832, whole genome shotgun sequence.
ACCESSION   DBNHOX010000430 DBNHOX010000000
VERSION     DBNHOX010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1062832"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1093
                     /gene="hisC"
                     /locus_tag="AC16UZ_09515"
     CDS             23..1093
                     /gene="hisC"
                     /locus_tag="AC16UZ_09515"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR6246961.1"
                     /translation="MKALKPYQPGKPVEEVTRELGLVDVIKLASNENPRGPGELVRQA
                     IDAACGDLARYPDGNAFALKQALARHFGVRESQLTVGNGSNDVLDLAARMSLSPGAEA
                     VISEHAFVVYRLATLSAGGELVEVPARNYGADLDGILDAITDATAIVFLANPNNPTGT
                     WVGEGALVRFLDEVPEHVWVVLDEAYHEYVEAAGYPNGMELQRRYPNLIVTRTFSKIH
                     GLAALRVGFSVSSEEAADLMNRARQPFNVNAPAMAAAVAALADQEFVQESRRLNREGM
                     GQMRDGIARLGLEQIPSVGNFLSFDVGGPCFPVYQALLEQGVIVRPIAEYGLPNHLRV
                     TVGLPEENARFLGALERVLGNS"
     gene            1098..1919
                     /locus_tag="AC16UZ_09520"
     CDS             1098..1919
                     /locus_tag="AC16UZ_09520"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR6246962.1"
                     /translation="MLLTVVGTGLIGGSFALGARTRNLFQTFVGVDPDPGNAQSALSL
                     GIVDEIADEVPAASDAVLIAAPSDAVAEWVVRLADHPGILFDAGSVKGAILDAIRGQL
                     GEIPERFVPCHPIAGREVSGPGAANEDLFAERLVVQTPVAGTVPEAREQVAYWWRSLG
                     ARVESMDPELHDRVYALTSHLPHLIAFAYMQQIEPAHLDHAGGGFRDFSRIGGSDPDM
                     WSAIFGLNKDAVLAALAGFEADLADLRRAIEADDEEAVRDCIARASERRRGYHGD"
     gene            1953..2627
                     /gene="cmk"
                     /locus_tag="AC16UZ_09525"
     CDS             1953..2627
                     /gene="cmk"
                     /locus_tag="AC16UZ_09525"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR6246963.1"
                     /translation="MSLPPVITVDGPSGSGKGTLAGLLAERLGWHLLDSGALYRIVGA
                     QAIARGLALDDEAAIAGMAAGLDIRFEDDRVLVDGEDLTRTIRTEAVSSAASQVAALE
                     GVRQAIFGLQRAMRRAPGLVADGRDMGTVVFPEADLKVYLEASPEVRAERRYNQLKNK
                     GLSVTFRALLASIRERDERDMRRAVSPLRPAQDAILVDSTELTIDQVLDRVLAEARTR
                     GLAKGI"
     gene            2706..>3012
                     /locus_tag="AC16UZ_09530"
     CDS             2706..>3012
                     /locus_tag="AC16UZ_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR6246964.1"
                     /translation="MSESFADLFEESLTTIEMEPGSIVTGTVVDIDNDWVIVHAGLKS
                     EGVIPRNQFINEEGEFTLSVGDQVQVAMEVVDDGWGETRLSREKAKRAESWMMLEDAF
                     "
BASE COUNT          501 a          938 c         1062 g          511 t
ORIGIN      
        1 gtcgccgcgc gttaacccca ggatcaaggc gctcaagccg tatcagcccg gcaagccggt
       61 agaagaagtc acccgggaac tcgggctggt cgacgtgatc aagctggcga gcaacgagaa
      121 ccctcgcggg cccggcgagc tggtgcggca ggccatcgat gcggcctgtg gagaccttgc
      181 ccgctacccg gacggcaacg cgttcgcgct caagcaggcg ctggcccggc atttcggggt
      241 gcgggagagc cagctcaccg ttggcaacgg ctccaacgac gtcctcgatc tggccgcgcg
      301 catgagcctg tccccggggg ccgaagcggt gatctcggaa cacgccttcg tcgtctaccg
      361 cctggccacc ctgtccgcgg gtggcgagct cgtcgaggtg ccggctcgga actacggcgc
      421 ggatctcgac ggaattctgg atgccatcac cgacgccacg gccatcgtct ttctcgccaa
      481 ccccaacaac cccaccggca cctgggtggg cgaaggggcg ttggtgcggt tcttggacga
      541 ggttcccgag cacgtctggg tcgttctgga cgaggcctat cacgaatacg tggaggcggc
      601 cggataccct aacggcatgg agcttcagcg gcgctatccg aacctgatcg tcacgcgcac
      661 gttctcgaag attcacgggc ttgccgccct tcgagtgggc ttcagcgtga gctccgagga
      721 agcggccgac ctgatgaacc gggcccgcca acccttcaac gtcaacgcgc cggccatggc
      781 tgctgccgtt gcggcgctcg cggatcagga gttcgtgcag gagagccgaa ggctcaaccg
      841 cgaggggatg gggcagatgc gcgacggcat tgcccggctt ggcctcgagc agattccgtc
      901 cgtgggcaat ttcctcagct tcgacgtggg cggcccctgt tttcccgtct accaggcgct
      961 gctcgagcag ggcgtcatcg tacggcccat cgccgagtac ggccttccca accatttgcg
     1021 cgtgaccgtc gggctgcccg aggagaacgc tcgctttctg ggggcgctgg aacgcgttct
     1081 cgggaattct taggccgatg ctgctcaccg tcgttgggac cggactcatc ggcggctcgt
     1141 tcgccctcgg cgcgcgcacc cgtaatcttt tccagacctt cgtcggggtc gatccggacc
     1201 cgggcaacgc gcagtctgcc ttgagcctcg gcatcgttga tgagatcgcc gacgaggtcc
     1261 ctgccgccag cgatgcggtg ctgatcgccg cgccgagcga cgcggtggcc gagtgggtgg
     1321 tgcggctggc cgatcacccc ggcattctgt tcgatgccgg cagcgtcaag ggggccatcc
     1381 tggatgcaat cagggggcag ctgggcgaga ttcccgaacg cttcgtgccc tgccatccca
     1441 ttgccggccg ggaggtcagc ggaccgggcg cggccaacga ggacctcttc gcggagcgcc
     1501 tggtggtgca gacgccggtg gcaggcacgg tcccggaggc gcgggagcag gtggcctact
     1561 ggtggcgcag cctgggcgct cgcgtggaaa gcatggaccc tgagctgcac gatcgcgtgt
     1621 acgcactgac cagccacctg ccgcatctca tcgcgttcgc ctacatgcag cagatcgagc
     1681 ctgcgcatct ggaccacgct ggtgggggct ttcgcgactt cagccgcatc ggcggttcgg
     1741 acccggacat gtggtcggcg atcttcgggc tgaacaagga cgcggtcctt gctgcgctgg
     1801 cggggttcga ggcggatctc gccgacctgc gtcgagccat cgaggccgac gacgaagagg
     1861 cagttcgcga ctgcatcgcc cgggcgagcg agcggcggcg gggctatcac ggtgactgag
     1921 gcggccgcgg tgatgattgg aggcgatggc cgttgagtct cccgccggtg atcacggtgg
     1981 atgggccgag cggctccgga aagggcacgt tggccggcct gcttgccgag cgtctcgggt
     2041 ggcacctgct ggatagcggg gcgctgtatc gcatcgtcgg ggcccaggcc attgcgcgag
     2101 ggctcgccct ggacgacgaa gcggccattg ccgggatggc ggcggggctc gacatccgtt
     2161 tcgaggacga ccgcgtgctg gtggatggcg aggatctcac ccggaccatc cgcaccgagg
     2221 ccgtcagctc ggcggcgtcg caggtggccg cgctcgaagg cgttcggcag gcgattttcg
     2281 gcctgcagcg cgccatgcgg cgcgctcctg gactggttgc cgacggccgg gacatgggca
     2341 cggtggtgtt cccggaggcg gatctgaagg tgtatctgga ggcctcgccg gaggtcaggg
     2401 cggagcgccg ctataatcaa ttgaaaaaca aagggttaag tgttaccttc cgcgcccttc
     2461 tcgcgagcat ccgtgaaaga gatgagcggg atatgaggcg tgccgtatcg ccgctgcgac
     2521 ctgcccagga cgcgatcctc gtggacagca ccgagctgac catcgatcag gtgctggaca
     2581 gggtgctggc cgaggcccgg acacggggcc tggcgaaagg catttgaaat gaaccaaccg
     2641 ggcggtatca ctggggtgct gctcgacaac acggagacgt cgcgccggcc ggcgcggcaa
     2701 attaaatgag cgaaagtttt gccgatctgt ttgaagaaag cctgacgacc atcgagatgg
     2761 aaccgggttc catcgtcacc ggcacggtgg tggatataga caacgactgg gtcatcgtcc
     2821 acgcggggct caagtccgag ggggtcatcc cccgcaacca gttcatcaac gaagagggtg
     2881 agttcaccct ctccgtgggc gaccaggtgc aggttgccat ggaggttgtc gacgacggct
     2941 ggggtgaaac ccgcctgtcc agggagaaag ccaagcgcgc ggaatcctgg atgatgctcg
     3001 aggacgcctt ca
//
LOCUS       DBNHOX010000431        14386 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_419526, whole genome shotgun sequence.
ACCESSION   DBNHOX010000431 DBNHOX010000000
VERSION     DBNHOX010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 14386)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14386)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14386
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_419526"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2601
                     /locus_tag="AC16UZ_09535"
     CDS             <1..2601
                     /locus_tag="AC16UZ_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246965.1"
                     /translation="AVIAGEDFDFELSLIAKALTLTASGHMDDLGRPRRPALKVNLMS
                     PDVDLITDALGAEEFGDGGLDLNIELTPGTDTVALTATGDLAGLRVRVDAAADDLSSM
                     RESSLNLSVQGPRFGRVMDVFGLEGLPDDPFQASGQVKRSGPVLTLSDVLLEVEGTRL
                     EIEGQTRGFPGIDAASLRLRLEGRELSRFQRFLSLPDWAVGPFTVAGSIEHSEGQTDT
                     LDLAAATSLANVSLRGPIGDPPEYGGTELSLTASGRDLRALVKVTESLEPTALEAVPA
                     KPFSSGGRLTVKRRVFELSAVYLEIEESRFDLEGSLSRRADLVGSRVTVTGRGGELES
                     LIADAAGLDFKPAPFEVTATLERTPETLQIQDLLLTNERGGEVRLNAELGLPFPASRA
                     ADVNLVARGPDVSALPVQPQRLELEPLPFEALLRASLRGDRLTVDKGSLVLGEAELDW
                     RGTFDLPPEFSATESTLSVNVPELAALGRLDGEPLPAAPLSLSARASGTSTTLRLEGI
                     EAELGASALSGDLQLDVGGTRPRLQAKIHIPTLDVPVSEDAQEARRPPPADGRLIPDV
                     ALPVDGLDVMDGALELTVDELRFGDLTFTQGFLSAVLEDGNLLVERLDIRPPSGRVQG
                     RLAVRPEDESGTIDLELRGDRVALNVAANLEPSAEPADQLVFDVDVYLRARGTDARAL
                     AASLEGEVSALSDGGRIHNRQLALIFGGFGRELLGNINPFIKHDPYTDLSCVVFRFSA
                     TDGILKADPYLAIRSDKLNLVSSGFANLATERIDFTFNALPRLRLSISATELINPYVR
                     VSGTLLKPTLSLDSRSAAVTGGAAALTGGLSLLAQATWKRTFGSRDACGKALEELRAE
                     EGAAAAVTPP"
     gene            complement(2588..3598)
                     /locus_tag="AC16UZ_09540"
     CDS             complement(2588..3598)
                     /locus_tag="AC16UZ_09540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6246966.1"
                     /translation="MDNGRWTVASRPEDQVEEANFQFVSESVGEPEDGEFLIGTRYLV
                     VSPPARMALTSGGIARRPVPIGATMRGTGLGQVVASRHPGFAEGELVIGDLGWQEYAI
                     SDGATRHPVRKVAARPGMPESTLLHVLGAGGATAYFGMIEYAKPHVGDTLVVSAAAGN
                     VGSLVCQLGRLQGCRVVGIAGSDEKCSWLTNDLGCAAAINYKREDVAAALDGACPDGI
                     DVYFDNVGGEILDAALARIAQGARVVLCGGTSQYNHDLDWYGPKNYFNLVYRQAEMAG
                     FYVYNFSHRFEEAFGRLESLLATGELKYAEDVLQGIGQTPAALIRVLKGENFGVQLVR
                     VA"
     gene            complement(3603..4493)
                     /locus_tag="AC16UZ_09545"
     CDS             complement(3603..4493)
                     /locus_tag="AC16UZ_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6246967.1"
                     /translation="MQRKDHIDTLAAVTLIVMNVLLGLNQSVVKLVNDGFAPVFQSGL
                     RSACAILPVLGYALVMRKRLSVTDGSLGLGMLNGLLFSGEFCLLFLALDYTSVARVSL
                     FFYVMPVWVTIGAHFLVPGERMTLLKAGGLVLALAGVALGLGIGSGSAGEQAWIGDLL
                     ALGGAIFWASIALLTRTTRLQRCSPEMNLLYQLAVSAVILLAVAPLFGDTVREPTPAI
                     LGMFAFQVIVIVAIGFVVWFWILSIYPVSHMASFSLLAPVFGVLFGWLIFDDQITPAF
                     VIALLLVGSGIVLINRSPAG"
     gene            4555..5586
                     /locus_tag="AC16UZ_09550"
     CDS             4555..5586
                     /locus_tag="AC16UZ_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HIR6246968.1"
                     /translation="MKRIVICADGTWNRPERNLAEDQPTNVLRLARAIRPSGSTGTAQ
                     QVFYDWGIGSYYDPFVGGVTGRGINKNIMDGYRYLVQNYAPGDQIFLFGFSRGAYTVR
                     SLCGLINNCGILKRPDARLIQAAFDHYKRPGSAHHPDGPRSREFRAEHAHPSRRIRFV
                     GVWDTVGALGIPFSFLGMLDREDEFYDTKIGPNIEIARHAMAIDERRSDFEPTIWQQR
                     TGTDISQVWFAGAHGNVGGGLYREGKGGRLSDIALAWMINEARGAGLSVEGHLGDRLR
                     PDPLAPVYGSRRHIYRSRPRADRPIDHGSAPVLVHESVRQRWDALPGYRPRNLVRYLE
                     INGWPTLVP"
     gene            complement(5601..6476)
                     /locus_tag="AC16UZ_09555"
     CDS             complement(5601..6476)
                     /locus_tag="AC16UZ_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007943000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR6246969.1"
                     /translation="MGAIEQPFEMIRYERPGEHVARIMLAREETRNAQNVQMLYEIDA
                     ALDAAMQDDDVRAVIIGADGPHFSSGHDLKGGGRIGDFGPPKMGVGGFGKPGQEGHMS
                     HEQEVFIGFCWRWRNMPKPTIAAVQGKAIAGGLMLVWPFDLIVASEDAQFSDPVVTFG
                     VNGHEYFVHAWEVGHRKAKEMLFTGESISAQEGKELGMVNRVVKREDLMSETLALAEK
                     IARQPSMGLKLSKLAVNQSLDAQGMWTAIQSAFNLHHLGHAHMRIMHGQAVEPAGAKK
                     IKTQARADKPKLTGP"
     gene            6611..7759
                     /locus_tag="AC16UZ_09560"
     CDS             6611..7759
                     /locus_tag="AC16UZ_09560"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HIR6246970.1"
                     /translation="MGTSGPARALRFGSATLTVTTMYTLLITFFTLAIVFSFLCSLWE
                     AVLLSITPAYAEIQKQHGSAIGRHLSTFKSNIDRPLAAILTLNTIAHTVGAIGVGGQA
                     AAIWAETSPWVTKAIIPVAMTLAILILSEIIPKTIGATFWKELAPFTVRSLLVVIAAL
                     GPLVWLSQRVTRILKREAGDSVLSRSDFLAMTKIGERAGVFEKSESEIIKNLLRFEQV
                     CAKDIMTPRTVVIAEPESRTVAEFYAAHDQLRFSRIPIFSDAKDHITGYVLKDELLSS
                     LVQQEGDRTLGDFRRDIMVAKEDQPLPDLFSRLVEKREHIALVVDEFGGMAGIVTMED
                     VIETLLGLEIVDESDQVYDMQAHARAQWARRAKAHGLTETDAVGPTSS"
     gene            complement(7778..8827)
                     /locus_tag="AC16UZ_09565"
     CDS             complement(7778..8827)
                     /locus_tag="AC16UZ_09565"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent amine dehydrogenase family
                     protein"
                     /protein_id="HIR6246971.1"
                     /translation="MALKVFQWASGTVGRHAALAVLERDGLELVGLHTLSAAKAGRDV
                     GELLGVAATGLRATSELEAVLESDADVVVHAPLASLVYGEDPGRDVEDICRLLASGKN
                     VITVVGYMYPKVYGPELVARLEDACRAGKSTFHSTGLNPGWMGDLVPLTMSALSRTID
                     RVHVLEISNFQGYPSPEIMFESMGFGSSPEEFERSGERRRNWLNGLFRESVQMVADGL
                     GLELDDVVEEMTLEFAPRDLETAAGTVRQGTVAGQHWEWAGVLGGDKRVIHETVWRMH
                     ADVAPDWPQGRHAVTVHGVPRIHLEFEADYVSDGLLATAMHAVNAIPYVCQSEPGIRT
                     FLDLPWIMYRGAGAP"
     gene            complement(8851..9960)
                     /locus_tag="AC16UZ_09570"
     CDS             complement(8851..9960)
                     /locus_tag="AC16UZ_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR6246972.1"
                     /translation="MSTTTALAAVAWAPDDPFAFEEVELGPLGADEVLVRIVASGICH
                     TDVAVKEQSVKLPLPMVLGHEGSGIVEAVGSAVSHLMPGDHVVLSSDSCGVCRRCHAG
                     LPSYCDEFVERNLTGFRTDGTSPLACRGEPLRGRFCGQSSFATAAISPGRSAVKVPQD
                     LPLELLGPLGCGLTTGVGTVMNALNPGPGSSIAVLGVGTVGLSAVMGAVLSGCEAIIA
                     VDPNLARLEVARELGATECIDAGREDVAADIVELTRGGADFSVECSGHPAAIDQAVAC
                     LGRPGWCAQVGAPTAGTRFPLDMDHVGFGRGIRGVVLGEANVHTFVPYLASLYRDGRL
                     PYDRLVRYYDFAEIDRAVHDSAVTGEVIKPILRMS"
     gene            complement(9957..11060)
                     /locus_tag="AC16UZ_09575"
     CDS             complement(9957..11060)
                     /locus_tag="AC16UZ_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6246973.1"
                     /translation="MEMRVLGRTGVSVSKLCFGAGMFGYFGNPDERECARMVDAAIDA
                     GVNYFDTSDVYSHGESESILGKALVGKRDDVILATKFSLPMSDDPNHRGNSRRWIARE
                     VEASLKRLQTDYIDLYQVHRPDVTADIDETLGALSDLVRSGKVRMLGSSTFPAEQIVE
                     AQWVAEKRHRERFVVEQPPYSIFARRIEADILPTCQRYGMGVVAWSPLSQGWLTGKWR
                     RDAPPNDTNRQKLQPHLYDMARADNQRKLDLVEQLAAVASEAGISLMHMAIAFVCAHP
                     AVTSAIIGPRTPEQLQGLLDGADVTLGDDVLDAIDAIVAPGTTLSRDDDGYVAPEIAN
                     KALRRRFVAEEVHEAARETLRNIEAYREQQGKK"
     gene            complement(11066..11587)
                     /locus_tag="AC16UZ_09580"
     CDS             complement(11066..11587)
                     /locus_tag="AC16UZ_09580"
                     /inference="COORDINATES: protein motif:HMM:NF025050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6246974.1"
                     /translation="MSIIRHPIWQYAYFVNDIDEACHKWNRMVGAGPFHVVRHHIAEG
                     FLYRGEHVEADVSYAFGQAGPAHIQFIAQHDDTPSIYRDMYAPGEQGFHHIGLLVHDV
                     DGEIKRFNEAGYETACTLWGGDYVAYMDCRQDMGCYVELHGDAPIIRDLFDGWKRQHE
                     EWDGVTDLIREQG"
     gene            11703..11882
                     /locus_tag="AC16UZ_09585"
     CDS             11703..11882
                     /locus_tag="AC16UZ_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246975.1"
                     /translation="MSVQNGERLIRFLRSDPQLREKVKDAGWEKFQDVTAEAGASCTT
                     YEVVAALIREIENDS"
     gene            11899..12240
                     /locus_tag="AC16UZ_09590"
     CDS             11899..12240
                     /locus_tag="AC16UZ_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246976.1"
                     /translation="MGNKLILVGLVEPQTDDLVEAFNQWYLGNHVEDTYNCPNITSVR
                     CFKAVRGFLGEPPSGYLTIYEFEGEDAEEAERILGQYQSDPDAWPEREPNNGSMKVVG
                     AGWYAEEIAFG"
     gene            12264..13091
                     /locus_tag="AC16UZ_09595"
     CDS             12264..13091
                     /locus_tag="AC16UZ_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6246977.1"
                     /translation="MPHVRINGARLWYEVRGSGEPLLLHHGYTASRVNWLPVAERLQD
                     RYQVVLMECRGTGDSEHTAEGYSLAQYAADVVGMLDHLDLERVTFAGHSMGGGIGYLL
                     GLDHAHRLDRLVLMAPIPAAGIAGLDPDARQKRLKLRRDQNRDALLAEYRAARFRPDV
                     ETDDWIESRVDHVLGVSDGHYEASAESMVELDVGHRLPELEVPTLMLAGAADELLRAN
                     LHDFLALPNATLEVLSRAGHEVAVHEPDAVARAIDAFMTHGPVTARHIRQQMEGESR"
     gene            13088..13987
                     /locus_tag="AC16UZ_09600"
     CDS             13088..13987
                     /locus_tag="AC16UZ_09600"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6246978.1"
                     /translation="MSAPPTRRRWQVADAFGDGNIEIAGLDWGGSGPLALLHHANGFC
                     AATWTLVARGLAGSHRVIAIDARGHGDSGTRPIPEGYQWDFFASDLTQVARQLLEETG
                     QPSVEFGIGSSMGGIVTAASEARNPGLFQRIAMLDPPLHPLPELRARLGLGVAAPNAD
                     LVAMTRKRKTIWPSRAIARQAWQDKPLFREWVPAAFDAYLEEGLGDRPDGTVALKCDP
                     GVEAAIFETTGSMDTFELAPRVSAPVLLVRALQGRAPGAAFEHLAGLLPRCTYREVDA
                     GHLLPMESPELTLALLAEFTEGG"
     gene            complement(14018..>14386)
                     /locus_tag="AC16UZ_09605"
     CDS             complement(14018..>14386)
                     /locus_tag="AC16UZ_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HIR6246979.1"
                     /translation="SNDGASPFEIERDIADSTLVTPRLDDESTLPAFGHLFAGGYAAG
                     YYSYKWAEVLAADAFAAFKEAGLDDGAAMKEIAGRFRDTVLGLGGSLPAGDVYRRFRG
                     RDASVRALLADQGLVAAEAG"
BASE COUNT         2598 a         4869 c         4602 g         2317 t
ORIGIN      
        1 gccgtgatcg ccggggagga cttcgacttc gagctctccc tgatcgccaa agcattgacc
       61 ctgaccgcct cggggcacat ggacgacctc gggagacccc gccgtccggc cctgaaggtg
      121 aacctgatga gccccgacgt cgacctgatc accgatgccc tgggcgcgga agagttcgga
      181 gatggcgggc tggatctgaa catcgagctg accccgggca ccgatacagt ggcgctgacg
      241 gcgaccggcg atctggccgg tctccgggtg agggtcgacg ccgcggccga cgacttgagc
      301 tcgatgcgcg aatcgagcct gaacctgagc gttcagggac cccgcttcgg gcgcgtgatg
      361 gacgttttcg gtctggaggg gctgccggac gatccgttcc aggcgtcggg tcaggtaaaa
      421 cgctccggcc ccgtattgac gctgagcgac gtgctgctgg aggtggaagg cacccgcctc
      481 gagatcgaag ggcagacgcg cggatttccg ggaatcgatg cagcgtccct gaggcttcgc
      541 ctcgagggtc gggaactcag ccggttccag cgtttcctct cccttccgga ctgggcggtg
      601 ggtcccttta cggttgcggg ttccatcgag cactcggaag gccagacgga caccctggac
      661 cttgccgccg ccacctcgct ggccaacgtc agcctgcgag gccccatcgg cgatccaccc
      721 gagtacggcg gcaccgagct gagcctgacc gccagcggca gggatctcag agcgctggtg
      781 aaggtgactg aaagcctgga accgactgct ctggaggcgg ttcctgccaa gccgttcagt
      841 tccggcggtc gcttgacggt aaagcgccgc gtcttcgagc tgagcgccgt ttacctcgaa
      901 atcgaggaat ccagattcga cctggaaggt tcgctctccc gcagggccga cctcgtcggc
      961 agccgtgtca ccgtcaccgg gcgcggcgga gagctcgaaa gcctgatcgc cgacgccgcg
     1021 ggcctcgatt tcaagccggc ccccttcgaa gtcaccgcca cgctggagcg cacgcccgaa
     1081 acgcttcaga tccaagacct tctgctcacg aacgagcgcg gcggcgaggt gcgcctgaac
     1141 gcggagctcg ggctaccttt cccggcgagc agggcggcag acgtcaatct ggtggcccgg
     1201 gggcccgacg tctctgcgct accggttcaa ccccaacgcc tggaactcga gccgctgccg
     1261 ttcgaggcgc ttctgcgcgc cagcctgcgc ggcgaccggc tcaccgtaga caaggggagc
     1321 ctggtcctcg gagaggccga gctcgactgg cgcggcacct tcgacctgcc cccggagttc
     1381 tccgccaccg aatcgaccct gtcagtgaac gtccccgagc tagcggcgct gggcaggctc
     1441 gatggcgagc cgttgccggc cgcgcctttg agcctgagcg cgcgcgccag cggcacgagc
     1501 accacgctga ggctcgaagg catcgaggcg gagctcggcg cctcagcgct ctccggcgat
     1561 cttcagctgg acgtcggcgg tacccggccg cgactgcagg caaagatcca catcccgaca
     1621 ttggacgtgc cggtgagcga agacgcgcag gaggcgcggc gcccgccgcc ggcggacggt
     1681 cggctgatcc ccgacgtcgc tctgccggtc gacggtctgg acgtcatgga tggggcgctc
     1741 gaactgaccg tggacgagct gagattcggc gacctgacgt ttacccaggg gttcctgtcc
     1801 gccgtgctgg aggacggcaa cttgctggtg gaacgcctcg acatccgccc acccagcggc
     1861 cgggtgcaag gccgtctagc ggtccggccg gaagacgaat ccggcaccat cgacctcgag
     1921 ctccgcggtg atcgggtggc gctgaacgtg gcggcaaacc tcgaaccgtc cgcggagcct
     1981 gccgaccagc tcgtgttcga cgtcgacgtg tatctgcgcg cccgaggcac cgatgcgcgg
     2041 gctctggcag catcgctgga aggcgaggtc agcgcgctga gcgacggggg ccggatccac
     2101 aaccgacagc tcgcgctgat cttcggcggg ttcgggcgcg agctgctcgg caacatcaat
     2161 cctttcatca aacatgatcc ttacacggat ttgtcctgtg tggtgtttcg attctccgca
     2221 acggacggca ttctgaaagc cgatccgtac ctcgcgatac gctccgacaa gctgaacctg
     2281 gtcagcagcg gattcgccaa cctggccacc gagcgcatcg atttcacctt caacgcgctg
     2341 ccaagactcc gactgagtat cagcgccacc gagctgatca acccctacgt gcgggtgagt
     2401 ggaaccctgc ttaagccgac cttgagcctg gacagccgaa gcgcggcggt gacgggcggc
     2461 gcggcggcat tgaccggagg tttgtccctg ctcgctcagg ccacctggaa gcgcactttc
     2521 ggctcccgcg acgcctgcgg caaggccctg gaggaactgc gcgcggaaga gggcgcagca
     2581 gcagccgtca cgccaccctg accagctgca cgccgaagtt ctcgcctttg aggacgcgaa
     2641 tcagcgctgc cggcgtctgc ccgattccct gcagcacgtc ctcggcatac ttgagctctc
     2701 ccgtcgcgag cagggactcc agccgcccaa aggcctcctc gaagcggtgg gagaagttgt
     2761 agacgtagaa tccggccatt tcagcctggc gatagaccag attgaagtag ttcttcggtc
     2821 cgtaccagtc caggtcgtgg ttgtactgag acgttccgcc acacagcacc accctggcgc
     2881 cctgggcaat gcgcgcaagc gcagcgtcga ggatctctcc cccgacgttg tcgaagtaga
     2941 cgtctatgcc gtcagggcag gctccgtcca gggctgcggc gacgtcttca cgtttgtagt
     3001 tgatcgccgc cgcgcagcct agatcgttgg tgagccagga acacttctcg tcgctgcctg
     3061 ctatcccgac tacccggcag ccctgcagcc ggcccagctg acagaccagc gagccgacgt
     3121 tgcccgccgc cgccgagacc accagcgtgt cgcccacgtg cggcttggca tactcgatca
     3181 tgccgaagta ggcggttgca cccccggccc ccagcacatg cagcagggtg ctttccggca
     3241 tccccggtct ggctgccacc ttccgcacgg ggtggcgcgt ggcgccgtcc gagatggcgt
     3301 actcctgcca gccgagatcc ccgatgacga gttcgccctc ggcaaacccc ggatggcggg
     3361 aggcgaccac ctggcccagc cccgttccgc gcatggtggc cccgatcggc actggacggc
     3421 gcgcgatgcc gccagaggtg agcgccatcc gggccggcgg gctcaccacc agatagcgcg
     3481 tgccgatgag aaactcgccg tcctccggct cgcccaccga ctcgctgacg aactggaaat
     3541 tggcctcttc cacctggtcc tccgggcgcg acgcgaccgt ccatctgccg ttgtccatcc
     3601 actcatcctg ccgggctgcg gttgatcagc acgataccag atccaaccag cagcaaggcg
     3661 atcacgaacg ctggcgtaat ctgatcatcg aagatcagcc atccgaacaa gaccccgaac
     3721 accggcgcca gcaggctgaa ggaagccatg tgggagacgg gatatatcga caggatccag
     3781 aaccagacca cgaagccgat ggcgacgatc acgatgacct gaaacgcaaa catcccgagg
     3841 atggccggtg tgggctcacg caccgtgtcg ccgaacagag gcgccaccgc cagcagaatc
     3901 acggcggata cggcgagctg gtagagcagg ttcatctccg gcgagcatcg ctgcagccgg
     3961 gtggtacggg tgagcagcgc tatgctcgcc cagaagatcg cgcctcccag cgccagcagg
     4021 tctccgatcc aggcctgctc gccggccgag ccggagccga tgccaagacc cagggctacg
     4081 ccggccagcg cgaggacaag gccgccggcc ttgagcagcg tcatccgctc gcccggaacg
     4141 agaaagtggg cgccgatggt cacccatacc ggcatgacgt agaaaaacag ggagaccctg
     4201 gccacgctgg tgtagtccaa agcgaggaag agcaggcaga actcgccgga gaaaagcagg
     4261 ccgttgagca tccccaggcc caggctgcca tcggtgaccg agagacgctt gcgcatcacg
     4321 agcgcgtagc cgagaacggg caggatggca cacgcggagc gcaatcccga ctgaaacacg
     4381 ggagcaaatc catcgttgac aagcttgacc acggactggt tcagtcccag caggacgttc
     4441 atgacgatga gcgttacggc cgccagagtg tctatgtggt ctttgcgctg catggggcag
     4501 catgatagcc gcagtgaacc gaacgggtat caacgaaacc cggggaggga gcaagtgaag
     4561 aggatcgtca tctgtgcgga tggcacatgg aaccgaccgg aacgcaacct ggccgaagat
     4621 cagcccacca acgtcctgcg gctggccagg gccatccggc catcgggcag cacgggcacc
     4681 gcccagcagg tgttttacga ctgggggatc ggctcttact acgatccctt cgtcggcggc
     4741 gtgacgggac ggggaatcaa caagaacatc atggacggat accgctacct ggtgcagaac
     4801 tacgcccccg gcgaccagat cttcctgttc ggcttcagcc gcggcgccta cacggtccgc
     4861 agcctgtgcg ggctgatcaa caactgcggc atcctcaagc gccctgacgc caggctcatc
     4921 caggcggcat tcgatcatta caaacgacca ggcagcgccc atcatcccga cggcccgcgc
     4981 tcccgggagt ttcgagcgga gcatgcgcac cccagccgca ggatccgctt cgtaggcgtc
     5041 tgggacaccg tcggtgcgct gggcattccg ttctccttcc tggggatgct cgatcgtgag
     5101 gatgagttct acgacaccaa gatcggtccc aacatcgaga tcgcccgcca cgccatggcc
     5161 atcgacgagc gccgctccga cttcgaacct acgatctggc agcagcgcac gggcaccgac
     5221 atctcacagg tttggtttgc aggcgcccac ggaaacgtcg ggggcgggct ctaccgggaa
     5281 ggcaagggtg gccggctttc ggacatcgcg ctggcatgga tgatcaacga agcccggggc
     5341 gccgggcttt cagtggaggg ccacctgggt gaccggctgc ggcccgatcc cctcgcgccc
     5401 gtgtacgggt cacgccgcca catctaccgc tcccggcccc gagcggatcg tcccatcgac
     5461 cacggcagcg ctccggtgct cgttcacgaa agcgttcgcc agcgctggga tgcgctgcca
     5521 ggctaccggc cgcgcaacct ggtccgctac cttgagatca acggctggcc gacgctggtt
     5581 ccctgagccg gcggctcgct tcaagggcca gtgagcttcg gcttgtccgc ccgggcctga
     5641 gtcttgatct tcttagcgcc ggcgggctcc acggcctggc cgtgcatgat gcgcatgtgg
     5701 gcgtggccga ggtgatgcag gttgaacgcc gattgaatgg ccgtccacat gccctgagca
     5761 tccagggact ggttgacggc gagcttggac agcttcagcc ccatgctggg ctgccgagcg
     5821 attttttctg caagggccag ggtctcgctc atcaggtctt cccgcttgac cacgcggttg
     5881 accatgccga gttccttacc ctcctgggcg gaaatcgact ccccggtaaa cagcatctcc
     5941 ttggccttgc ggtgcccgac ttcccaggcg tgcacgaaat actcatggcc gttgacgcca
     6001 aaggtgacca cggggtcgga gaactgcgca tcctcgctgg cgacgatgag atcgaaaggc
     6061 cataccagca tgagccctcc ggcgatggcc ttcccctgca cggcggcgat ggtcggcttg
     6121 ggcatgttgc gccagcgcca gcagaagccg atgaagacct cctgctcgtg agacatgtgg
     6181 ccttcctgcc cgggcttgcc gaaaccgccg acgcccattt tcgggggtcc gaagtcgccg
     6241 atgcggcccc cgcccttcag gtcgtggccc gacgagaaat gcgggccgtc ggcgccgatg
     6301 atcaccgccc gcacgtcgtc gtcctgcatg gccgcatcca gcgccgcgtc gatctcgtag
     6361 agcatctgga cgttctgcgc gttgcgggtc tcttcccgcg ccagcatgat gcgcgccacg
     6421 tgctcgccgg gccgctcgta gcggatcatc tcgaaaggct gctcaatggc acccatgaca
     6481 tccccccaat tcaaggcggg cccgcgcgcg tccgcggctc ggaccctgtt tgccggaagc
     6541 cgcgcccccg ggcacggcga ctgccgactc taatccctgg ccgatcagcc agcaacttcc
     6601 gcccggcacc atgggaacgt ccggtccggc cagggcgttg cgctttgggt ccgcaacgct
     6661 cacagtcacg accatgtaca cgctgctgat caccttcttt acgctggcca tcgttttctc
     6721 ttttctctgc tccctgtggg aggccgtgct gctgagcatt acgcccgcgt acgctgagat
     6781 tcagaaacag cacggctcgg caatcgggcg ccacctgagc actttcaaga gcaacatcga
     6841 ccggccgctg gccgccatcc tcacgctcaa caccatcgcc cataccgtcg gcgccatcgg
     6901 cgtgggtggt caggccgccg ccatctgggc ggaaaccagc ccctgggtca ccaaggccat
     6961 cattcccgtg gcgatgacgc tcgccattct catcctgtcg gagatcatcc cgaagaccat
     7021 cggcgccacc ttctggaagg agctggcgcc tttcacggtg cgctcgctgc tcgtggtgat
     7081 cgccgcgctg ggaccgctcg tctggctgag ccagcgggtc acccgaatcc tcaagcggga
     7141 ggccggagat agcgtcctct cgcgctccga ctttctcgcc atgaccaaga tcggcgagcg
     7201 ggccggcgtc ttcgagaagt cggagtcgga gatcatcaag aatctccttc gcttcgagca
     7261 ggtttgcgcc aaagacatca tgacgccgcg caccgtggtg attgccgagc cggaaagccg
     7321 caccgtggcc gagttctacg ccgcgcacga tcagctgcgc ttttcccgca tcccgatatt
     7381 cagcgatgcg aaagaccaca tcaccggcta cgtgctcaag gacgagctgc tgtccagcct
     7441 ggttcagcag gaaggtgaca ggaccctggg cgacttccgc cgcgacatca tggtggcgaa
     7501 ggaggaccag cccctgccgg acctgttctc gaggctcgtg gagaagcgcg agcacattgc
     7561 cctggtggtg gacgagttcg gcggcatggc cggcatcgtc accatggaag acgtgatcga
     7621 gacgctgctc ggcctggaga tcgtcgacga gtccgatcag gtctacgata tgcaggccca
     7681 cgcaagggcg cagtgggccc gacgcgcaaa ggcccatgga ctcacggaaa cggacgctgt
     7741 cgggccgacg tcttcctgac gacgcctggc ggcaggctca tggggcgcca gcaccacggt
     7801 acatgatcca gggcagatcc agaaacgtcc tgattcccgg ctcggactga caaacgtagg
     7861 gaatggcgtt caccgcatgc atggcggtgg cgagcagacc gtcgctgacg tagtcggctt
     7921 cgaactccag atgaatgcgc ggcacgccgt gcacggtgac ggcatggcgg ccctgaggcc
     7981 agtcgggcgc gacgtcggcg tgcatgcgcc agacagtttc gtgaatcacc cgcttatcgc
     8041 ccccgagcac gccggcccat tcccaatgct ggccggccac ggttccctgg cggacagtcc
     8101 cggctgcggt ttcgaggtcg cggggagcaa actcgagggt catctcttcc accacgtcgt
     8161 cgagctcgag cccaagaccg tcggccacca tctgcacgct ttcccggaag aggccgttga
     8221 gccagttgcg gcgccgctcc ccggatcgct cgaactcttc gggtgacgat ccgaacccca
     8281 tggactcgaa catgatctcc ggcgacgggt aaccctgaaa gttggagatc tccaggacgt
     8341 ggacgcgatc gatcgtccgc gacagcgcgc tcatggtcaa cggcaccagg tctcccatcc
     8401 agccgggatt caagcccgtg gagtggaagg tggacttacc cgcgcggcag gcatcctcca
     8461 gcctcgcgac cagctccggc ccgtagacct tcggatacat gtagcccacc acggtaatga
     8521 cgttcttgcc cgaggcgagc agcctgcaga tgtcctcgac gtcacgaccc ggatcctcgc
     8581 cgtaaacgag cgaggccagc ggcgcgtgaa cgaccacgtc ggcgtcggac tccagcacgg
     8641 cttcgagttc gctggttgcc cgcaggccgg tggcggcaac gcccagcagc tcacccacat
     8701 cccggcccgc cttggcagcg gacagggtgt gcaggccaac gagctccagg ccgtcacgtt
     8761 cgagcacggc cagggcagcg tgacgcccga cggtgccgct ggcccattga aaaaccttca
     8821 gggccatggg atcccctcca aatacctgcg ctaggacatt ctcagtatgg gcttgatcac
     8881 ctcaccggtg accgcgctgt cgtgaacggc acggtcgatc tcggcgaaat cgtagtaccg
     8941 caccagccga tcatagggca gccgcccgtc gcggtagagc gaagcgaggt agggcacgaa
     9001 agtgtgcacg ttggcctcgc cgaggaccac gccgcgaatg cccctgccga accccacatg
     9061 atccatgtcc agagggaaac gggttcccgc ggttggcgcg cccacctgag cgcaccagcc
     9121 cggacgcccg agacacgcca ccgcctgatc aatggccgca gggtgacccg agcactccac
     9181 ggagaaatcg gcgccgcccc gggtcagctc tacgatatct gcggcgacat cctcgcgccc
     9241 cgcgtcgatg cactccgtgg cgccgagctc gcgggccacc tccagccgcg ccaggttggg
     9301 atccacggcg atgatcgcct cgcagccgga gagcaccgcg cccatgacgg cggaaaggcc
     9361 caccgtgccg acgcctaata cggcgatgct cgaacccgga cccgggttca gagcattcat
     9421 cacggtgccg acccccgtgg tcagaccgca gccgagcggg ccgagaagct caagcggcag
     9481 atcctggggc accttcaccg cgctgcgtcc gggcgagata gcggcggtcg caaacgaaga
     9541 ttggccgcag aagcgtccgc gcagaggctc gccccggcag gccagcggac tcgttccatc
     9601 cgtgcgaaag ccggtgagat tgcgctccac gaactcgtcg cagtaggagg gaagcccggc
     9661 gtggcagcgc cgacagacgc cacatgagtc actgctgagg accacgtgat cgcccggcat
     9721 gaggtgcgag accgcgctgc caacggcctc gacgatcccg gatccttcgt ggccgagaac
     9781 catgggcaac ggcagcttga ccgactgctc cttgacggca acgtcggtgt ggcagatgcc
     9841 cgaggctacg atccggacca gcacctcgtc cgcgcccagc ggccctagct ccacctcctc
     9901 gaaggcaaac gggtcgtccg gcgcccaggc gacggctgca agagcggtgg tcgtgctcat
     9961 ttctttccct gctgttcgcg gtacgcctcg atgttcctga gggtttcccg cgcggcctcg
    10021 tgaacctcct cggccacgaa ccggcggcgc agggccttgt tggcgatctc cggggccaca
    10081 tagccgtcgt cgtcgcggct gagggtagtc cccggagcga cgatggcatc tatggcatcg
    10141 agcacgtcgt cgccgagggt cacgtcggcg ccatcgagca gcccctgaag ctgctcgggc
    10201 gtgcgcgggc cgatgatcgc cgaggtcacc gcgggatggg cgcagacgaa ggcgatggcc
    10261 atgtgcatca ggctgatgcc cgcttcgctc gcaaccgccg ccagttgctc caccaggtcg
    10321 agcttgcgct gattgtcggc tcgggccatg tcgtacaggt gcggctgcag cttctgccgg
    10381 ttggtgtcgt tgggcggcgc gtcgcggcgc cacttgcccg tgagccagcc ttgcgacaac
    10441 gggctccacg caacaacccc catgccgtaa cgctgacagg tgggcaggat gtccgcttcg
    10501 atgcgccggg cgaagatcga gtagggcggc tgctcgacga cgaaacgctc gcggtggcgt
    10561 ttctcggcaa cccactgggc ttccacgatc tgctctgcgg ggaacgtgga cgagcccagc
    10621 atgcggacct tgcccgagcg aaccagatcc gaaagggcgc ccagagtctc gtcgatgtcg
    10681 gcagtcacgt ccggccgatg aacctgatag agatcgatgt aatccgtctg cagacgtttc
    10741 aggctcgcct cgacctcgcg cgcgatccag cgccgcgagt tgccgcgatg gttcgggtcg
    10801 tcgctcatcg gcaggctgaa cttggtggcg agaatcacgt cgtcgcgctt gccgacgagc
    10861 gccttgccga gaatggattc cgactcccca tgggagtaca cgtcggacgt gtcgaaataa
    10921 ttgacgcctg cgtcgatggc ggcatccacc atgcgcgcgc attcgcgctc gtcgggattg
    10981 ccgaaatagc cgaacatgcc cgcaccgaag cagagcttgc tgacactgac gcccgtacgg
    11041 ccgagaaccc gcatctccat gacgctcagc cctgctcgcg aatcagatcc gtgacgccgt
    11101 cccactcctc gtgctggcgc ttccagccat cgaagagatc gcgaatgata ggcgcatcac
    11161 cgtgcagctc cacgtagcaa cccatgtcct gacggcaatc catataggcc acgtagtcgc
    11221 cgccccagag ggtgcaggcg gtctcgtagc ccgcctcgtt gaaccgcttg atctccccgt
    11281 cgacgtcgtg cacgagcagc ccgatgtggt gaaagccctg ctcgccgggc gcgtacatgt
    11341 cccggtagat cgagggcgtg tcgtcgtgct gagcaatgaa ctgaatgtgt gcagggccgg
    11401 cctggccgaa cgcgtagctc acgtccgcct cgacgtgctc gccgcgatac aggaaacctt
    11461 cggcgatatg atgccgcacg acgtggaacg gtcccgcgcc caccatgcga ttccacttgt
    11521 gacaggcttc atcgatgtcg ttgacgaagt aagcgtactg ccagatggga tgccggataa
    11581 tgctcatgat gtctcccctt tgaaatccac ctcaaacctt gccgaacgat agcagactaa
    11641 cccgacgccg ccgagtgctg tagactcggc tacgaaaccg acatctcgaa aagggggggg
    11701 acatgtccgt acagaacggc gagcggctca ttcgtttcct tagaagcgac ccgcagctgc
    11761 gggaaaaggt caaggacgct ggatgggaaa agtttcagga cgtcactgcc gaggccggtg
    11821 cctcctgcac gacctacgag gtcgtcgccg cgctcattcg cgagatcgag aacgacagct
    11881 gaacaggaaa gacgattcat gggcaacaaa ctgatcctcg tgggcctggt agagccgcag
    11941 acggatgacc tggtcgaggc cttcaatcag tggtacctgg gcaaccacgt ggaagacacc
    12001 tataactgcc cgaacatcac gtcggtgcgc tgcttcaagg ccgtgcgggg gttcctgggc
    12061 gagccgccct ccgggtacct gaccatctac gagttcgaag gcgaggacgc cgaggaggcg
    12121 gaacgcattc tcggtcagta tcagtccgat cccgacgcct ggcccgagcg cgagccgaac
    12181 aacggatcga tgaaggtcgt gggcgcaggc tggtacgcag aggagatcgc cttcggctga
    12241 gcccgagcgc ggcgaccgta aaaatgcccc atgtccgcat caacggggcc cgtctgtggt
    12301 acgaggtccg aggctccggc gagcccctgc tgctgcatca tggctacacg gcgtcacggg
    12361 tcaactggct gcccgtggcc gagcgtctgc aggaccgcta tcaggtcgtt ctcatggagt
    12421 gccggggtac cggagatagc gagcacaccg ctgagggata cagcctcgcc cagtatgcgg
    12481 ccgacgtcgt cggcatgctg gaccacctcg acctggaacg ggtgaccttt gccggccaca
    12541 gcatgggcgg cggcatcggc tacctgctgg gcctcgatca cgctcaccgg ctggatcgcc
    12601 tggtgctcat ggcgcccatt cccgccgcgg gcatcgccgg cctcgatccg gacgcgcggc
    12661 agaagcgcct gaagctgcgc cgcgaccaga accgcgatgc cctgctggca gagtaccggg
    12721 ccgcccgctt tcgcccggac gtggaaaccg acgactggat cgagagccgc gtggaccacg
    12781 tgctcggcgt cagcgacgga cactacgagg cgagcgcgga gtccatggtc gagctggacg
    12841 tgggccaccg actgccagag ctggaggtac ccaccctcat gctcgccggc gcagccgacg
    12901 agctgctgcg cgcaaacctt cacgacttcc tcgccctgcc caacgccacc ctggaggtgc
    12961 tctccagagc aggccacgag gtggccgtgc acgagccgga cgccgtggcc cgggccatcg
    13021 acgccttcat gacccacggc cccgtcacgg cccggcacat caggcagcaa atggaggggg
    13081 aatccagatg agcgcccctc ccacccgtcg cagatggcag gtagccgacg ccttcggcga
    13141 cgggaacatc gagatcgccg gtctggactg gggaggcagc gggccgctgg cgctgctgca
    13201 tcacgcgaac gggttctgcg ccgcaacctg gacgctcgtt gccagaggcc ttgccggcag
    13261 ccatcgggtc atcgccatcg atgcccgggg ccacggcgac tcgggaacac gccccatccc
    13321 cgaaggatac cagtgggact tcttcgcctc ggatctcacc caggtggcca ggcaactgct
    13381 cgaggaaaca gggcagccat ccgtcgagtt cggcatcggc agctccatgg gcggcatcgt
    13441 caccgccgcc tcggaagcac gcaacccggg gctcttccag cgcatagcca tgctggaccc
    13501 gccgctacat ccgctacccg agctgcgcgc caggctcggg ctcggcgtcg cggcgccaaa
    13561 cgcggacctg gtggcaatga cgagaaagag aaagacgatc tggccgtccc gcgcgatcgc
    13621 ccggcaggcg tggcaggaca agccgctttt ccgcgaatgg gtaccggccg cattcgacgc
    13681 ctacctcgaa gaggggctcg gcgaccgccc ggatgggacg gtggcgctga agtgcgaccc
    13741 cggcgtggaa gccgctatct tcgaaaccac gggttctatg gacaccttcg agctcgcccc
    13801 gcgtgtgagc gcgcccgtgc tgctcgtccg cgccctgcag ggccgggccc ccggagccgc
    13861 cttcgagcac ctcgcaggcc ttctgccgcg ctgcacctac agggaggtgg acgccggaca
    13921 cctgctgccc atggaatctc ccgagctgac actggcgctg cttgccgagt tcaccgaggg
    13981 cggctgagcc gtcggcaacc gcttaggctc gggcgcgtca gccagcctca gccgcgacca
    14041 ggccctgatc ggccagcagc gcacgtaccg aggcgtcccg cccccggaaa cgccggtaga
    14101 cgtcccccgc cgggaggctt ccgccaagac cgaggacggt gtcccgaaag cgcccggcaa
    14161 tttctttcat cgccgcgccg tcatcgagtc ccgcctcctt gaaggccgca aaggcatccg
    14221 ccgcaaggac ctcggcccac ttataggagt agtagccggc cgcgtagcca ccggcgaaga
    14281 gatgaccgaa ggcaggcagc gtactctcgt cgtcgagcct cggcgtgacg agggtgctgt
    14341 cggcgatgtc ccgctcgatc tcgaaggggc tcgcgccgtc gttgct
//
LOCUS       DBNHOX010000432         4848 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_741280, whole genome shotgun sequence.
ACCESSION   DBNHOX010000432 DBNHOX010000000
VERSION     DBNHOX010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_741280"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="AC16UZ_09610"
     CDS             complement(<1..963)
                     /locus_tag="AC16UZ_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014543.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HIR6246980.1"
                     /translation="MTRHLLFAAAGLLLAACSTVSIDGPTAPRGDPAAGRLTLPSGRT
                     VWVYPNDLAFTGQARYEWPDGRAYAGGWLSGRPHGTGLMRSDDGERYSGMWNEGRRHG
                     HGELTNPNGDHYVGSFVNGLREGAGTQKSQDGFYRGEWAGDLPNGEGRFNANDGSLYQ
                     GQWLDGERAGTGTYTDASGNRYEGEWLADVPHGFGILTEQDGSEYDGRWTAGSRDGYG
                     ASRNALDMAYEGTWNAGNYHGFGILLRPDGSSYEGEWHHGKRQGQGRETDPEGGFHDG
                     AWENNQPLGPGTRRDVAGVEIAGMWTGSRVRTGLVKLPTGLEYAG"
     gene            1042..2076
                     /locus_tag="AC16UZ_09615"
     CDS             1042..2076
                     /locus_tag="AC16UZ_09615"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6246981.1"
                     /translation="MTASKDDGSIEGVDIPRVTHWLAGRIAGLRPPLEFSLIAGGHSN
                     LTYRFADQAGAEYVLRRPPLGHVLESAHDMAREHRIVSALAGTEVPVAPTHGLCEDRD
                     VNGAPFYVMSYVRGAVLHDADAARELDPAERHALGEDVINVLARLHALDPDAVGLGTL
                     GRKEAYLARQLKRWKKQWEASKTHEIPEMEETARLLEENMPEQIGAAIVHGDYRLGNM
                     IVADGRILAVLDWELCTLGDPLADVGYLLNNWIQPEDGAGELVPTAAGGFPSREALCD
                     GYTAATGRDLSGINYYRAFSHWRLAAIGQGVYKRYLVGAMGERPDMDLDTYKASVQQR
                     AAAALELLTA"
     gene            complement(2073..2543)
                     /locus_tag="AC16UZ_09620"
     CDS             complement(2073..2543)
                     /locus_tag="AC16UZ_09620"
                     /inference="COORDINATES: protein motif:HMM:NF025701.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HIR6246982.1"
                     /translation="MTRSRTILGALLVAALYGCSGRDDVSLVEVLNHDQCRMSQAGTE
                     VVSIEDVARIRGGRLLSAPDEPATTPTDLLLVAVSRGQQPTPGYGFELVAGTMKGDAV
                     TVRLAWNQPPADAVLAQVITNPCIVIGLEGEGFSRLRVIDQDDTPLGEIVLPGG"
     gene            complement(2540..>4848)
                     /locus_tag="AC16UZ_09625"
     CDS             complement(2540..>4848)
                     /locus_tag="AC16UZ_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR6246983.1"
                     /translation="PERGKLFQDGDPGDFYAMTMVGNEFIYLNIADPENAEQELLLYD
                     SQQNVISADGGPGEVQVAEVPAAGDYFIEVRPIAGASNYVLTVGQDLVPERATTMIDP
                     FLPREIILRTTDDEIAAEYGLSMLAQSGPRMLLKAGPATAALRHIDRRAPFDASWTED
                     QQDRYATLAVIEALGRDPRVELAEPNLIRKPHREPGDSFYRSQWHYQAINLPLAWDIT
                     TGDGDVIVAVVDTGIRADHPDLDEKVIAGFDFIANLQRAADGDARDDDPTDPGDGAFG
                     GSSSFHGTHVAGTVAAETSASVTPASGVAGVAWEAKIMPLRVLGVGGGTSFDVIESMR
                     WAARLDNVSGTLPDRAADVINLSLGGGGFLESEQQAIDEIRAAGTIIVASAGNDASAS
                     PSYPAAYDGVVSVSATTIENNPAAYSNFGTTVDVAAPGGSNVTDVNGDGIGDGVISTM
                     ADDSDPSSLRFGYASLQGTSMAAPHVAGVVALMKAVHPGLTPIQFDNALASGRITDDL
                     GAPGRDDRYGWGLINAQKAVLAALDLASNPPGPTLAVSASSLTFGAALSSQSLRLENV
                     GSGTVTVQSIGTAASWLDVAPSSVLPGGMGSYTVTVDRTGLTPGSYTGAIEIVTDAVN
                     PTATVTATMRVFTTSPDANAGVHYVVLVDAAGETLPSPQNVLEATNGEYAYRLDGVAP
                     GDYRIFAGTDSDDDAFLCDAGEACGTYGTLDSPAVVTVDGEDLTGIDFTSEFRVNLTN
                     LSANADVSGQVAPEAVRLPGKADGGAPR"
BASE COUNT          745 a         1595 c         1667 g          841 t
ORIGIN      
        1 accggcgtac tccagcccgg tgggcagctt tacgagcccg gtccgcaccc ggctgccggt
       61 ccacatgccg gcaatctcca cgccggcgac gtcgcgacgc gtacccgggc cgaggggctg
      121 gttgttctcc caggccccgt cgtggaagcc tccttcgggg tccgtctcgc ggccctgacc
      181 ctgccgcttg ccgtgatgcc actcgccttc gtagctgctg ccgtccgggc gcagaagaat
      241 gccgaagccg tgataattgc ccgcgttcca cgtgccctcg taggccatat ccagggcgtt
      301 ccgcgaagct ccatagccgt cgcggcttcc ggccgtccat cggccgtcgt attcggatcc
      361 gtcttgctcg gtgagaatgc cgaagccgtg gggcacgtcg gcgagccatt ccccttcgta
      421 gcggttgccg ctcgcgtcgg tgtacgtccc ggtgccagca cgctcgccgt cgagccactg
      481 gccctggtag aggcttccat cgttggcatt gaagcggccc tcgccgttcg ggagatcgcc
      541 cgcccattcg ccccggtaga agccgtcctg ggatttttgg gttcctgcgc cttctcgcag
      601 gccgttgaca aagctgccca cgtaatgatc gccgttcggg ttcgtcagct cgccgtggcc
      661 gtggcgtctg ccctcgttcc acatgccgct gtagcgctca ccgtcgtcgc tgcgcatgag
      721 cccggtgccg tgtggcctgc cggacagcca gccgcccgca taggcgcgcc cgtcgggcca
      781 ttcgtagcga gcctgcccgg tgaatgccag atcgttcggg tagacccaca cggtgcgccc
      841 ggagggcagc gtgagcctgc cggcagcggg atcgcctcgg ggggcggtgg gaccgtctat
      901 ggagaccgta gagcatgccg ccaggagcag acctgcggca gcgaagagca gatgtcgcgt
      961 cactgggtta tggaaaacga tctcatgtca aaactcaaag gctttgggta accttgcccg
     1021 ggtctggaac gggagaattc cgtgaccgcg agcaaagacg acggcagcat cgaaggcgtc
     1081 gacatcccgc gcgtgaccca ttggctggca ggccggatcg ccggcctgcg gccgccgctg
     1141 gagttttcac tgattgcggg cggtcactcg aacctcacct accgctttgc cgatcaggct
     1201 ggcgccgagt acgtgctgcg tcgcccgccc ctgggccacg ttcttgaaag tgctcacgac
     1261 atggcccggg agcatcgtat cgtctccgcg ctcgcgggca ccgaggttcc cgtggcgccc
     1321 acccatggcc tttgtgagga ccgggacgtc aacggggcgc ccttctacgt gatgagctac
     1381 gtccgggggg ccgtgctgca cgatgcggac gccgcccgtg agctcgatcc ggccgagcgg
     1441 catgcccttg gcgaggatgt gatcaacgtg ctcgccaggc tccatgccct ggatcccgac
     1501 gcagtgggtc tcggcacgct ggggcgcaag gaagcctacc ttgcccggca gctcaagcgc
     1561 tggaagaagc agtgggaggc atcgaagacc cacgagattc ctgagatgga ggagacggcc
     1621 aggctgctcg aggaaaacat gcccgagcag atcggggcgg ccatcgtcca cggtgactat
     1681 cgcctgggca acatgatcgt cgccgacggc cgtattcttg ccgtcctcga ctgggagctc
     1741 tgcacgctgg gcgacccgct ggcggacgtc ggctaccttc tgaacaactg gattcagccc
     1801 gaggacggcg cgggtgagct cgtgccgacg gcggccgggg ggttcccgtc ccgggaggcc
     1861 ctctgtgacg gttacacggc ggcgacgggg cgggacctct cggggatcaa ctactaccgg
     1921 gctttttccc actggcgtct ggccgccatc ggtcagggcg tctacaagcg atacctggtg
     1981 ggcgccatgg gcgagcgccc ggacatggac ctggacacct acaaggcgag cgtccagcag
     2041 cgggcggccg ccgcgctcga gctgctgacg gcttagccgc cgggcagcac gatctcgccc
     2101 agcggggtgt cgtcctggtc gatgacgcgc agccggctga agccctcacc ttccagcccg
     2161 atgacgatgc agggattggt gatcacctga gcgagcacgg catccgcagg cggctggttc
     2221 caggccagcc gtaccgtgac cgcatcgcct ttcatggtcc ccgcgacgag ctcgaagccg
     2281 taacccggcg tgggctgctg gcctctggag accgcgacga gcagcaggtc cgtgggcgtg
     2341 gtggcgggct cgtcaggggc agacagcagc ctgcccccgc ggatgcgcgc cacatcctcg
     2401 atgctcacca cttccgttcc ggcctgggac atcctgcatt ggtcgtggtt gagcacttcc
     2461 accaaagaga cgtcgtcacg accgctgcag ccgtaaagcg cggcgacgag caacgcacct
     2521 agaattgtcc ttgagcgggt catctgggag ctcccccatc tgccttgccc ggcaagcgta
     2581 cggcttcggg tgcgacttgc ccagagacgt cggcgttcgc cgagaggttg gtcaggttga
     2641 cgcgaaactc agaggtgaag tcgatgccgg tcaggtcttc gccgtccacc gtgacgacgg
     2701 ccggagagtc cagagtcccg taggtgccgc acgcttctcc cgcgtcgcac agaaaggcgt
     2761 cgtcgtcgga gtccgtcccg gcgaatatcc ggtagtcgcc gggcgcgacg ccgtcgaggc
     2821 ggtaggcgta ctccccgttc gtggcctcca ggacgttctg aggtgacgga agggtctccc
     2881 ccgccgcatc gacgagcacg acgtagtgca cgccggcgtt ggcgtccggg ctggtggtga
     2941 aaacccgcat cgttgcggtg acggtggccg tggggttcac ggcatcggtc acgatttcga
     3001 tggcgccggt gtaggagccc ggggtaaggc ccgtgcggtc cacggtgacc gtgtaggaac
     3061 ccatgccgcc gggcagcacc gagcttggcg ccacgtccag ccaggatgcg gctgtgccga
     3121 tgctctgcac cgtcaccgtg ccggagccga cgttttccag tcgaagagac tgtgacgaca
     3181 gtgccgcccc gaaggtcagg gaactggctg acacggccag ggtcgggcca ggcgggttgc
     3241 tggccaggtc cagggcggcc agcaccgcct tctgggcgtt gatcaatccc cagccgtagc
     3301 ggtcgtcccg ccccggcgcg cccagatcgt cggtaatccg gcccgacgcg agcgcgttgt
     3361 cgaactggat gggggtcagg ccggggtgaa cggccttcat cagagcaacg acgccggcaa
     3421 cgtgaggcgc ggccatggac gtcccctgca gggatgcgta gccgaagcgc aggctcgagg
     3481 ggtcgctgtc gtcggccatg gtgctgatga ccccgtctcc gatcccgtcg ccgttgacgt
     3541 cggtgacgtt ggacccgccc ggtgccgcca cgtcgacggt ggtcccgaag ttcgaatacg
     3601 ccgccggatt gttctcgatg gtggtggcgc tgacggagac gaccccgtcg taagcggctg
     3661 gatagctggg cgaggcggac gcatcgttgc cggcgctggc aacgatgatg gtgcccgccg
     3721 cgcggatctc gtcgatggcc tgctgctcgc tttcgagaaa cccgccccct ccgagactca
     3781 ggttgatcac gtccgcggcg cggtctggaa gggtgccgga gacgttgtcg aggcgggcgg
     3841 cccatcgcat ggactcgatg acgtcgaagc tggtgccgcc gcccacaccg agaacccgca
     3901 ggggcatgat cttcgcctcc cacgcgacgc cggcaacgcc cgaggccggg gtgaccgagg
     3961 cggatgtttc cgccgccacg gttcccgcaa cgtgggtgcc gtgaaagctg ctggaaccgc
     4021 cgaacgcccc gtcgcccgga tcggtcggat cgtcgtcgcg agcatccccg tcggctgccc
     4081 gctgcagatt ggcgatgaag tcgaagccgg cgatgacctt ctcgtcgagg tcggggtgat
     4141 ccgcccggat gcccgtgtcg accaccgcga cgatcacgtc cccatcgccc gtggtgatgt
     4201 cccacgccag aggcaggttg atggcctggt agtgccactg ggaccggtag aaagagtcgc
     4261 ccggctcccg gtgtggcttc cgaatcaggt tgggttccgc aagctcgacg cggggatcgc
     4321 ggcccagcgc ctcgatgacg gccagggtgg cgtatcgatc ctgctgatct tcggtccacg
     4381 aggcatcgaa tggtgcccgc cgatcgatgt gccggagcgc ggccgttgcc gggccggctt
     4441 tcagtagcat ccgcgggccg gactgagcga gcatgctgag gccgtactcc gccgcgatct
     4501 catcatcggt cgtgcgcagg atgatctctc gtggcaggaa cggatcgatc atggtggtgg
     4561 cccgctcggg gacgaggtcc tgacccaccg tgagcacgta gttgctggcg ccggcgatgg
     4621 ggcgaacctc gatgaagtag tctccggcgg cgggaacctc ggcgacctga acctctcccg
     4681 gcccgccgtc ggcgctgatc acgttctgct gcgagtcgta aaggagcagc tcctgctccg
     4741 cgttctcggg atcggcgatg ttcaggtaga tgaactcgtt gccgaccatg gtcatggcgt
     4801 agaaatcgcc gggatcgccg tcctggaaga gctttcccct ttccggct
//
LOCUS       DBNHOX010000433         2930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1383981, whole genome shotgun sequence.
ACCESSION   DBNHOX010000433 DBNHOX010000000
VERSION     DBNHOX010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1383981"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="AC16UZ_09630"
     CDS             <1..305
                     /locus_tag="AC16UZ_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246984.1"
                     /translation="DPVGAMPGNLEQWTALARHLGDEQDRGRLRNDIRAGTAAMLYLA
                     LAALPALLPAHVRAILGSNDAAMLQGDLRKLLNPPAELHGGSGRPRVRMQPQVRPR"
     gene            complement(308..1585)
                     /gene="aroA"
                     /locus_tag="AC16UZ_09635"
     CDS             complement(308..1585)
                     /gene="aroA"
                     /locus_tag="AC16UZ_09635"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR6246985.1"
                     /translation="MGSLTIQPIQALEGDITLPGSKSISNRALLLAAEAQGQTRLVNL
                     LDSDDTAYMLRALGQLGISITENGGDHLVTGVAGPLVNDERHESLDLGLAGTALRPLT
                     AALTLGRGVFELDGTARMRERPVGHLVEGLRQLGASIRYTGAEGFPPIEVTGTGLLGG
                     RVTMQGNVSSQFLTSLLLAAPLAAGPVEVVVEGEQVSKPYLDITVDMMRRFGATVGHE
                     DHRLFRVEPGGYQSPGRYLIEGDASAASYFLAAGAIRGRGVRVHGIGAGSVQGDMAFV
                     EVLKAMGARVTVDAEWVRVEPGALTGVDLDLNHIPDAAMTLAVLALFAEGSTRIRNIY
                     NWRVKETDRLAAMATELKKLGAEVDECEDSLTVNPPKYMNAASIDTYDDHRMAMCFSL
                     AALGGVAVTINDPDCVSKTFPRYFAEFERLARR"
     gene            complement(1632..2222)
                     /locus_tag="AC16UZ_09640"
     CDS             complement(1632..2222)
                     /locus_tag="AC16UZ_09640"
                     /inference="COORDINATES: protein motif:HMM:NF018996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein Syd"
                     /protein_id="HIR6246986.1"
                     /translation="MSVQTALSQFVSHYEKSYPELTEIFDPEWRSPCETSEPYQDDDG
                     IVRVPWSPLARGLKVPEEHDFAGLENALETEIHPDIKAYYGSFWSGGLEAEAPQGHVS
                     LILLWNAEDASRLIENLIGHALAKRRARAPLSVFFACTDQDSELFLSVENTTGEVLIE
                     KPGHKPVDVVAGSLASFIDSLVPAPPELHPERSLLR"
     gene            complement(2261..2749)
                     /locus_tag="AC16UZ_09645"
     CDS             complement(2261..2749)
                     /locus_tag="AC16UZ_09645"
                     /inference="COORDINATES: protein motif:HMM:NF025173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HIR6246987.1"
                     /translation="MPRSKWLVLLLVLSLGANLAMAGFLAGRATGDGGGPRLLDPNLR
                     VTRMLLQQLPEERREALRPLVRDQVRSIRPSVGGIREAQRDIEAALVAEPFHPEKLEE
                     ALGAFRTHLSRSQETSHAAFVGLARSLSPEERRLLGKALAGSGRHRPHGPPPPGMPAV
                     RP"
     gene            complement(2739..>2930)
                     /locus_tag="AC16UZ_09650"
     CDS             complement(2739..>2930)
                     /locus_tag="AC16UZ_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6246988.1"
                     /translation="DIWQRAADWFASAFWPPALTGACALLLGFATGTLLPAANEEALI
                     DDVAGLAFTETYLEFEDAP"
BASE COUNT          489 a          932 c         1014 g          495 t
ORIGIN      
        1 tggatccggt cggggcgatg ccgggaaacc tggagcagtg gacggcgctg gccaggcacc
       61 tgggtgatga gcaggacagg ggccggttgc gcaacgatat ccgggccggt accgcggcga
      121 tgctttatct cgccttggcc gccttgcccg cgctgttgcc ggctcacgtg cgggccatct
      181 taggctccaa cgatgccgcg atgctgcagg gggaccttag aaagcttctg aatccgcccg
      241 ccgagctcca cggcggcagc gggcgtccca gggtacgcat gcagccccag gttcgtccgc
      301 gctgaggtca gcggcgtgcg agacgctcga actccgcgaa gtagcgcgga aacgtcttgc
      361 tgacgcagtc cgggtcgttg atcgtgaccg ccacgccgcc caacgcggcc agcgagaaac
      421 acatggccat gcggtggtcg tcgtaggtgt cgatagaggc tgcgttcatg tattttggag
      481 gattgactgt aagtgagtcc tcacattcat ctacctcggc acccagcttc ttaagctccg
      541 tcgccatggc ggcaaggcgg tccgtctcct tgacccgcca gttgtatatg ttccggattc
      601 gtgtggaacc ctcggcgaag agcgcgagca cggccagcgt catggcggca tcggggatgt
      661 ggttcaggtc cagatcgacg cccgtgagtg ctcccggctc gacccgcacc cattcggcgt
      721 ctacggtcac ccgcgccccc atggccttta ggacctcgac gaaggccatg tcgccctgga
      781 cggaacctgc tccgatgccg tgcacgcgga cgccccggcc acggatggcg cccgcggcga
      841 ggaaatagga agcggcggat gcgtcgcctt cgatgaggta gcgcccgggc gattggtagc
      901 cgccgggctc gacgcgaaac aggcgatgat cctcgtggcc gacggtcgcg ccgaagcggc
      961 gcatcatgtc tacggtgatg tcgagatagg gcttggacac ctgctcgccc tccacgacga
     1021 cctccaccgg tcccgcggcc aggggggcgg cgagcagcag ggaggtcagg aactggctgg
     1081 acacgttgcc ctgcatggtc acgcgcccgc cgagaaggcc ggtgccggtc acctcgatgg
     1141 gcgggaagcc ctccgcgccg gtgtagcgga tgctcgcgcc gagctggcgc agcccttcca
     1201 ccaggtgccc gacgggccgt tcgcgcattc tcgcggtgcc gtccagctcg aatacgcctc
     1261 ggcccagggt cagtgctgcg gtgagtggcc ggagagcggt gccggccagg ccgagatcca
     1321 gagactcgtg gcgctcgtcg ttgaccaggg gcccggccac gccggtcacg agatggtcgc
     1381 cgccgttctc ggttatggat attcccagct gccccagggc gcgaagcatg taggccgtgt
     1441 cgtcgctgtc gagcaggttc acaagacggg tctggccttg ggcttcagcg gccaggagca
     1501 gggcgcggtt cgaaatgctc ttggagcccg gcagcgtgat gtcgccttcc agagcctgga
     1561 tgggttgaat ggtcagagat cccatggcgt gggattaaac gacaaaccgg gtgcgggcgc
     1621 gaacctgcgc gttaccgcag cagactgcgc tcagggtgca gctccggagg tgcgggcacc
     1681 agcgagtcga tgaagctggc gaggcttccg gccaccacgt ccaccggctt gtgaccgggc
     1741 ttctcgatca gcacctcacc ggtggtgttc tccacggaga gaaagagctc cgagtcctga
     1801 tcggtgcagg cgaagaagac gctgagaggc gccctggctc gccgtttggc cagcgcgtgc
     1861 ccgatgaggt tctcgatcag ccgggaagca tcctctgcgt tccacagaag aatgaggctt
     1921 acgtgaccct ggggcgcctc tgcctcgagc cctcccgacc agaagctgcc gtagtaagcc
     1981 ttgatgtccg ggtgtatttc agtctccagg gcgttctcca ggccggcgaa gtcgtgctcc
     2041 tcgggaactt tcagcccgcg ggcaagcggg ctccagggta cccggacgat gccgtcgtcg
     2101 tcctggtagg gctcgctcgt ctcgcagggc gagcgccatt cgggatcgaa tatctccgta
     2161 agctcggggt aggacttctc gtagtgggag acgaattggg aaagcgcggt ctggacggac
     2221 aacgaagatc tcgcttggaa tcgaggcggt attttacctg ctacgggcgc acggcaggca
     2281 tgccgggggg cgggggcccg tgaggccgat ggcggcctga gcccgccagc gccttcccga
     2341 gcaggcgacg ctcctcgggt gagagagatc gcgcaagccc gacgaaggcg gcgtgactgg
     2401 tttcctggct gcggctcaga tgggtacgga acgccccaag cgcctcctcg agcttttcgg
     2461 ggtggaacgg ctcggccacc agcgcggctt cgatatcccg ctgagcctca cggatcccgc
     2521 cgacgcttgg ccggatcgac ctgacctggt cgcgcacgag cgggcgcagc gcctcgcggc
     2581 gctcctccgg aagctgttgc agaagcatcc gggtcaccct cagattggga tcgagcagac
     2641 gaggtccgcc cccatcaccg gttgcgcgtc ccgccaggaa tccggccatg gcgaggtttg
     2701 cgcccagcga caggaccagc agaagaacga gccatttgct acggggcatc ttcgaactcc
     2761 agataggtct cggtaaacgc aagccctgcg acgtcatcga tcagggcttc ttcgtttgcc
     2821 gccggcagca gcgtgcccgt ggcgaagccc agcagcagcg cgcaagcgcc ggtcagcgcc
     2881 ggtggccaga acgcggaggc gaaccagtcg gccgcgcgct gccagatgtc
//
LOCUS       DBNHOX010000434         3346 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1448230, whole genome shotgun sequence.
ACCESSION   DBNHOX010000434 DBNHOX010000000
VERSION     DBNHOX010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1448230"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="AC16UZ_09655"
     CDS             <1..315
                     /locus_tag="AC16UZ_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6246989.1"
                     /translation="FETKIAAMKYNGLRYLTKQIKGEPLSSETSVNKLHRASLEIEMD
                     DFAVELTGQAGLLLSGSEEAVSGGSWAKSSLSWPNVVIGGGTPNIQRNIIAERILGQP
                     KD"
     gene            complement(342..941)
                     /locus_tag="AC16UZ_09660"
     CDS             complement(342..941)
                     /locus_tag="AC16UZ_09660"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6246990.1"
                     /translation="MSVDVRIGLISDTHIPEARKTLWPQVFTAFDGVDMIMHAGDIHE
                     LYVLDALEEVAPVVAARGNGEDGGGGRPVQPDDPRVKYAWLLELGGLWVGLTHYVPVP
                     ERPPQFTLARWVERYFPERTPDIIVSGDTHREIIAEVQGVLCVNPGSPTYPHNYDTQY
                     GTLGFLDLVDGEARPSVWQITDGGIEPFDWDAVPPWKRR"
     gene            complement(938..2206)
                     /locus_tag="AC16UZ_09665"
     CDS             complement(938..2206)
                     /locus_tag="AC16UZ_09665"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6246991.1"
                     /translation="MSSRNIYQALEVPDFRWLWVGSLASSFAMNMQIVARGWLVYTLT
                     SSALDLAWVTLSFMVPMVAFSLWGGVVADRLPKRTIIAIAQALNTLATIVMGVIIFSD
                     LVSFWDFVWFGFFNGTVLALSMPARQAFAPELLPDRLIFTAMALNTTSWNLSRILGPA
                     FAGFLIAWVAAGDTYSTYGVGIVYVVIAALYFLSMATVLLVRKPGIVSSREQRKSPLT
                     DMADAFRYVWANPPVFGLIVLSIVPFLFGMPINTLLPAFNEDILGGGPDDLGLLMSTM
                     GMGAIVGSLLLAGVNDFRHRAAWLIATCVGWGAFTCAFGFSQTLMLSAGLLAVIGFLS
                     SWNMSLNRGLLQLQVDDHMRGRILSVDMMSHGLMPLGVIPISLIAEQFSVATALVVSG
                     AIFIVVVLLLLVVSPSIKRVDDDLQPVSGG"
     gene            complement(2309..3151)
                     /locus_tag="AC16UZ_09670"
     CDS             complement(2309..3151)
                     /locus_tag="AC16UZ_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6246992.1"
                     /translation="MLKWQIGEVSVTQIVELSTASLGPYLLPQADPGALLEVPWLRPF
                     IDENGRIVLSVHALVVESEGERLIVDTCIGNDKERTYPRWNRMQTDFLDRLAQAGYPP
                     ETIDTVMCTHMHVDHVGWNTRLTDDGWVPTFTRARYLFAEDEWAYWQREPQEFGPVVE
                     DSVQPIFDAGLAEVVGQTHRVTDEVWLEPTPGHTPGHVSVHIASAGEEAIITGDMIHH
                     PCQIAHPEWSSSADWNQEASAETRDAFLERYADRPVLVIGTHFAAPTAGHIVRDGESF
                     RLDY"
BASE COUNT          683 a         1086 c         1068 g          509 t
ORIGIN      
        1 ttcgaaacca agatcgccgc gatgaagtac aacggtttgc gttacctgac caagcagatc
       61 aagggcgagc cgttgtccag cgagacatcg gtgaacaagc tgcatcgcgc aagcctcgag
      121 atcgagatgg acgacttcgc ggtggagctc accggtcagg ccgggctcct gctctccggc
      181 agcgaggaag ccgtgtcagg aggaagctgg gcaaagtcga gcctttcctg gccgaacgtg
      241 gtgatcggcg gcggcacgcc caatatccag cgcaacatca tcgccgagcg catcctcggg
      301 cagcccaagg actgacctag ccgggccaac gggcccgccg gtcagcgacg cttccagggc
      361 ggcacggcgt cccagtcgaa gggctcgatg ccgccgtcgg tgatctgcca gacgctgggc
      421 cttgcctccc cgtcgacgag gtcgagaaac cccagcgtgc cgtactgggt atcgtagttg
      481 tggggatagg tgggcgagcc cggattcacg cagagcacgc cctgcacctc cgcgatgatc
      541 tcccggtggg tgtcccccga gacgatgata tcgggcgtcc gctcgggaaa gtaccgttca
      601 acccacctcg ccagagtgaa ctgtggcggg cgttcgggca ccggcacgta atgagtcagg
      661 cccacccaga gacccccgag ttccagcagc caggcatact tcacgcgcgg atcatccggc
      721 tgcacgggtc gaccgccgcc accatcctcc ccgttgcccc gggcggcaac caccggggct
      781 acctcctcca gggcatccag gacgtacagc tcgtgaatgt cccccgcgtg catgatcatg
      841 tcgacgccgt cgaaggcggt gaagacctgc ggccagaggg ttttgcgcgc ctccggaatg
      901 tgtgtgtctg atatcaggcc gatgcgcacg tcgacgctca cccgcccgag acgggctgca
      961 ggtcatcgtc cacccgcttg atcgaagggg acaccaccag aagcaacagc accaccacga
     1021 tgaagatcgc tccgctgacc accagcgccg tggccacgct gaactgttcg gcaatgaggc
     1081 tgatgggaat gacgcccagc ggcatgagcc cgtgggacat catgtcgacg ctgagtatcc
     1141 gcccccgcat gtggtcgtcc acctgaagct ggagcagccc gcggttgagc gacatgttcc
     1201 aggagctcag aaagccgatc accgcgagca gcccggcgga gagcatcagg gtctgggaga
     1261 agccgaacgc gcaggtgaag gccccccagc ctacgcaggt agcaatgagc caggccgcgc
     1321 ggtgccggaa gtcgttcacg ccggccagca gcagggatcc gacgatggcg cccatgccca
     1381 tggtcgacat cagcagcccc agatcgtccg gccccccgcc cagaatgtcc tcgttgaacg
     1441 ccggcagcag ggtgttgatg ggcatgccga acagaaacgg cacgatggag agaacgatca
     1501 ggccgaatac cggcgggttg gcccagacgt aacgaaaggc gtccgccata tcggtgagcg
     1561 gggacttgcg ctgctcgcgg gaactcacga tgccgggctt gcgtaccagc aggacggtcg
     1621 ccatggaaag gaagtagagc gcggcaatca cgacgtagac gatgcctacc ccgtaggtgg
     1681 agtacgtgtc gcccgccgcg acccacgcga tgagaaagcc tgcaaacgcg gggccgagaa
     1741 tgcgtgagag gttccagctg gtggtgttca gcgccatggc cgtgaagatc agcctatccg
     1801 gcaggagctc gggcgcgaac gcctggcggg cgggcatgga aagcgcgaga acggttccat
     1861 tgaagaaacc gaaccagacg aaatcccaga aagacaccag atcggagaag atgatcacac
     1921 ccatgacgat ggtcgccagg gtgttcaacg cctgcgcgat ggcgatgatg gtgcgcttcg
     1981 ggagccggtc ggccaccacg ccaccccaca gcgagaaggc caccatgggc accatgaacg
     2041 acagggtcac ccaggccagg tcaagagccg acgacgtcaa cgtgtagacc agccagcccc
     2101 gggcgacgat ctgcatgttc atggcgaaag acgaagccag gctgcccacc caaagccagc
     2161 ggaagtcggg cacttccaga gcttggtaga tgtttcgtga cgacaacggc ggatccggta
     2221 gggtcgggca agcccgtcac tttagcacag ccgcggggcg tcaccgtccc agcccgaacc
     2281 ccgcgggagg gccggccggg caataaaatc agtagtcgag ccggaagctt tccccgtcac
     2341 gcacgatgtg cccggcagtc ggcgcggcaa aatgggtccc gatcacgagc acgggacggt
     2401 cggcatatcg ctcgagaaac gcatcgcgcg tctcggcgct ggcctcctga ttccagtccg
     2461 cgctgctcga ccattccgga tgagcgatct ggcagggatg atggatcatg tcccccgtga
     2521 tgatggcttc ctcgccggcg gaagcaatgt gcacgctcac gtgaccgggc gtgtgcccgg
     2581 gcgtcggctc gagccagacc tcgtccgtga cccgatgggt ctgccccacc acctcggcga
     2641 gcccggcatc gaagatcggc tgcaccgagt cctccaccac ggggccgaac tcctgcggct
     2701 cgcgctgcca gtaggcccac tcgtcctcgg cgaagagata gcgcgcccgg gtgaaagtgg
     2761 gtacccaccc atcgtccgtc agccgcgtgt tccagccaac gtgatcgacg tgcatgtgcg
     2821 tgcacatcac cgtgtctatg gtctccgggg gatacccggc ctgagcgagc cgatccagaa
     2881 aatcggtctg catgcggttc cagcgcgggt aggtgcgctc cttgtcgttg cctatgcagg
     2941 tatcgacgat cagccgctcc ccttcggact ccaccacgag ggcatggacg ctcaacacta
     3001 tccggccgtt ctcatcgatg aacggccgaa gccagggtac ctcgagaagc gcacccggat
     3061 cggcctgggg cagcaggtac gggccgagcg aggctgtgga gagctccacg atctgcgtga
     3121 ccgaaacctc accaatctgc catttcagca tggaatcccc ctgacctcac cccgacccgg
     3181 cgagccttgc agatcggctg acggcggtca aatcccgccg ttccccggcc ttgggcgcgg
     3241 catgccatgt cataatgtcg ctcgtcagct acataccccg ggggagagat catgaccaaa
     3301 ttcggcgaca tcgcggtaga tcggaagagc acacgtctga actcca
//
LOCUS       DBNHOX010000435         6225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_741409, whole genome shotgun sequence.
ACCESSION   DBNHOX010000435 DBNHOX010000000
VERSION     DBNHOX010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_741409"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="AC16UZ_09675"
     CDS             complement(<1..220)
                     /locus_tag="AC16UZ_09675"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR6246993.1"
                     /translation="MREAWIIDAVRSPRGRGKPGVGSLSNIHPQRCMAQVLNGLRDKV
                     GFDASDIEDVVAGCGTGSGDHAHDIARMS"
     gene            complement(315..944)
                     /locus_tag="AC16UZ_09680"
     CDS             complement(315..944)
                     /locus_tag="AC16UZ_09680"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009394438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6246994.1"
                     /translation="MIDLYTWPTPNGRKISIALEEFGEPYRVHAVDIGAGDQHGSAFR
                     RISPNGKIPAIVEDSSGRSLMESGAILLYLAEKHDRFRGDDWWQTVEWLMLQMASVGP
                     MLGQVHHFARFNPGKAPYAEARYLDEARRLYGVLDRRLADREFLSGSYSIADMASWPW
                     ISRYEWQGIDLADYAHVRRWYVSIAGREAVQRGYHVPHFVNPVPMPDGA"
     gene            complement(946..1713)
                     /locus_tag="AC16UZ_09685"
     CDS             complement(946..1713)
                     /locus_tag="AC16UZ_09685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6246995.1"
                     /translation="MIARRNSQRPNSAELLEFGEYVKVLEASGEREDVVAFFDLDRTI
                     IAGYSITALMIERLRSGMSPRHLVAQVSRFISYGFGRAGYQELLEGTVAELVGLPEQE
                     LVELGERAYQRRLQRLIYPEARRLIGAHKELGHEVVMVTSATRYQAQPVARELAVDQI
                     CCTELEIRQGRITGRVSPCFGASKRAAAVHLGSARACSLADAYFYTDSCDDLPLLEAV
                     GRPVAANPKASLSEVATSRGWPQLAFRRQGEAGSVAA"
     gene            complement(1825..2793)
                     /locus_tag="AC16UZ_09690"
     CDS             complement(1825..2793)
                     /locus_tag="AC16UZ_09690"
                     /inference="COORDINATES: protein motif:HMM:NF025598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIR6246996.1"
                     /translation="MSARIGWLAALLAVQLLLTGALLLLEGRGADSSPAAFVEVAPDS
                     VDRVVVGGEETELEFTRAGEQWSLGSGAPADAEKIGEVLEKLTDIEPGWPVAATEASR
                     ERFRVTGDDHERRVALFAGEGKLAEIYLGTSPGFRRIHARRADSEEVYSIDFAVYELP
                     LSDSDWLDKDLLGADGEIASIIREEHWSLERGQEGWLLNLVPGMQDAEAGPGIGGADQ
                     DAAGRLVERVAELRVTGFAPLGPAYEPAAVFTVTDASGSYGLRIFKDPENDEYAVESD
                     RLSGRFGLAAYVAEQLVVEHADLRAAEPQDEAPAPGSEGFSDAGPG"
     gene            complement(2790..5702)
                     /locus_tag="AC16UZ_09695"
     CDS             complement(2790..5702)
                     /locus_tag="AC16UZ_09695"
                     /inference="COORDINATES: protein motif:HMM:NF021351.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HIR6246997.1"
                     /translation="MRPDVVKRIAGKELTLFFASPVGYLFLGAFLAFTLFVFFWVEAF
                     FARNVADVRPMFEWMPVLLVFLSAALTMRMWSEERRTGTLEFVATVPVSGWEFVVGKF
                     LACWVLLAIALLLTLPLPISVSLIADLDWGPVWAGYLAAMLLGGAYLAIGLFVSARTD
                     SQIVSLILASFACGAFYLLGSPVLTDLVGGGLADILRSVGSGSRFESITRGVLDLRDL
                     YYYASLLAVFLAANVHALEVQRWAADGGAGRHRRWLLGTGLLVANLLLANVWLAQLTG
                     LRIDVTEGRIYSVSDATRAYIGQLREPLLIRGYFSNRTHPLLAPLVPRMRDLLNEFEV
                     AGAGNIRVEIVDPADDPAAEDEANSKYGIRPVPFQVADRYESSLVNSYFDVLLSYGDE
                     YEVLGFRDLIEVKVVGEADLDVQLRNPEFDLTRAIKKVLYGFQGGGSLFANIQDPVRF
                     VGYVSSADRLPPALADYRAVLVEALDALARESGGRFTSEFIDPDAGDGSVAAQIAVDF
                     GFQPMAASLFDSNVFYFYLTLGDANTLIQLPLPEAFSVEATRRVLEEGLKRFASGLLK
                     TVALAAPEAPPYMGMGQPPQGNQYEQLKDVLRSDFEVETTDLAEGVVPDSADLLMIVD
                     PEGFNRRQLFAMDQFLMKGGTVVVATAPFSASLSQQSLMAAPRTSGLEAWLEHMGVTL
                     EPKLVMDAQNAAFPAPVTRQVGGFSFQELVMLDYPYFVDVRGEGMNAESPIVAGLPQV
                     TLTWASPIEIDAEQQEGRTVTELLTSSPRSWLSADTNVMPRLDEQGLSGFAPEGAQAP
                     RLLGALIEGRFESFFAGQTSPLLEPADGDDDNDAGADGDDETDPAATVGVVSSVIARS
                     ADSARLFLFSSDGFLTDQNLRMLGSAEGTIYTNTVQMMVNVVDWSLEDQSLLSIRSRG
                     HFNRTLPPLVDEEQLFWEYLNYGLALLGIGVVFLVHRGRKRRSDRMYASWLAGGAS"
     gene            complement(5692..>6225)
                     /locus_tag="AC16UZ_09700"
     CDS             complement(5692..>6225)
                     /locus_tag="AC16UZ_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6246998.1"
                     /translation="ISTLSRGFKQRVGVAQAILHEPRFVILDEPTNGLDPSQTQHMRE
                     LIQRLAATATVVLSTHIMQEVDAICDRALILRDGRLVLDERLADLKRSDQLRLRCAGG
                     GPVADLLAGVSGVSRAESVAEGAWQLTVDGPLDAAAERLARALVDAGIALHELGPVQR
                     DLETVFREVNEGVSDAA"
BASE COUNT         1141 a         2143 c         1979 g          962 t
ORIGIN      
        1 ccgacatgcg ggcgatgtcg tgggcgtgat cgccgctgcc ggtcccgcag ccggcgacga
       61 cgtcctcgat gtcggacgca tcgaaaccga ccttgtccct cagcccgttg agcacctgcg
      121 ccatgcagcg ctgagggtga atgttgctca ggctgccgac ccccggctta ccccgccccc
      181 gcggtgaccg caccgcgtcg atgatccagg cttcccgcat ggcgcctctc cgttgaatgt
      241 cagtggagcc gcaattctac accgggccaa ccgaggcttc accggcgccc gcggccacgc
      301 cggcggcgtg acgctcaggc gccgtccggc atggggacgg gattgacgaa gtgcggcacg
      361 tgataaccgc gctggacggc ctcgcggccg gcgatcgaga cgtaccagcg gcgcacgtgg
      421 gcgtagtccg caaggtcgat tccctgccat tcgtaacgcg atatccacgg ccagctcgcc
      481 atgtccgcga tagagtagct gcccgacagg aattcccgat ccgccaggcg ccgatcgagc
      541 acgccgtata ggcgccgcgc ttcgtcgagg tagcgcgctt cggcgtaggg ggctttaccg
      601 ggattgaatc tggcgaagtg atgaacctgc ccgagcatgg ggccgacact tgccatctgc
      661 agcatcaacc actccaccgt ctgccaccag tcgtcgcccc ggaagcggtc gtgcttctca
      721 gccaggtaga gcaggatggc cccggactcc atcaggctgc gcccggacga atcctcgacg
      781 atggccggga tttttccgtt cgggctgatt cgtcggaagg ccgagccgtg ctgatcgccc
      841 gcgccgatgt ccacagcgtg gacccggtag ggctcgccga actcttccag agcaatcgag
      901 atcttgcggc cgttgggtgt cggccaggtg taaaggtcga tcacctcagg cggccactga
      961 gcccgcctcg ccctgcctgc ggaacgcgag ctgcggccag ccgcgcgacg ttgcgacctc
     1021 ggaaagcgaa gccttggggt tggcagccac gggccgtccg acggcttcga gaagcggcag
     1081 gtcgtcacag gaatcggtgt agaaataggc gtccgccagg ctgcaggccc gagcggaccc
     1141 caagtgaacg gctgccgccc gtttgctcgc accgaaacac gggctgaccc tgccggtgat
     1201 cctgccctgg cggatttcca gctccgtgca gcagatctgg tcgacggcca gctcccgggc
     1261 gacgggctgc gcctgatacc gggtagccga agtgaccatg acgacttcgt gacccagctc
     1321 cttgtgcgcg ccgatcaggc ggcgcgcctc cgggtagatc agcctctgca gacggcgctg
     1381 gtaggcccgc tcccccagct ccaccagctc ctgctccggc agccctacga gctcggccac
     1441 ggttccttcg agcagttcct ggtaaccggc ccggccaaag ccataggaga tgaagcgact
     1501 gacctgagcg acgagatgcc gtggcgacat gccgcttcga agccgctcga tcatgagcgc
     1561 ggtgatggaa tagccggcga tgatggttcg atccagatcg aaaaacgcga ccacgtcctc
     1621 gcgctcgcca gacgcctcca gaactttgac gtactcgccg aactccagca gctccgcgga
     1681 gttcggcctt tgactgtttc gccgggcaat catggcaggc cctggtcgcc gttggtcggt
     1741 ttagctctca cgcgccgcgc cagcgcggtg cgctccgcac gtcggcgacg cctgaacaat
     1801 gagaccatcg aaacacgagt cgattcaacc ggggcccgca tcggaaaacc cttcggaccc
     1861 gggcgccggc gcttcgtcct gcggttcggc ggcacgcagg tcggcatgct ctacaaccag
     1921 ctgctcggct acgtaagcgg ccagaccgaa gcggccggac agacgatcgg attccacggc
     1981 gtactcgtcg ttctccggat ccttgaaaat ccgcagcccg taagacccgg atgcgtccgt
     2041 gacggtgaag accgcagcag gctcgtacgc ggggcccaac ggcgcaaatc cggtcacccg
     2101 aagctcggcg acccgctcga cgagccggcc cgcggcgtcc tgatcggcgc cgccgattcc
     2161 ggggccggct tcggcatcct gcatccccgg caccaggttc aggagccagc cttcctgacc
     2221 acgctccagg gaccagtgtt cctcccggat gatcgaggcg atctccccat ctgcccccag
     2281 caggtccttg tcgagccaat ccgaatcgct caacggcagc tcgtagacgg cgaaatcgat
     2341 gctgtatacc tcctcggaat ccgcccgtcg ggcatgaatg cggcggaatc ccggcgacgt
     2401 tcccagatag atctcggcca gctttccttc ccccgcgaag agggccaccc gtcgctcgtg
     2461 atcatcgccg gtaacgcgaa accgctcccg cgacgcttcg gttgccgcca cgggccagcc
     2521 gggctcgatg tcggtgagtt tttccagaac ctcgccgatc ttctctgcgt cggcaggggc
     2581 gccggagccc aacgaccatt gttcccccgc tcgggtgaac tccagctcgg tttcctcacc
     2641 gccgacgacc acccggtcca cgctgtccgg ggcgacctcc acaaacgccg ccggcgagct
     2701 gtccgcgcca cgaccttcga ggagcagtag ggcgccggtc agcaggagct gaacggcgag
     2761 cagcgcggca agccagccga tacgcgcgct catgaggcac ctcccgccag ccagctcgcg
     2821 tacatccggt cggaccggcg tttcctgccc cggtggacga gaaagaccac gccgatgccc
     2881 agcagcgcaa gcccgtagtt gaggtactcc caaaacagct gctcctcgtc gacgagcggc
     2941 ggcagcgtcc ggttgaagtg tccgcgtgag cggatgctca acaggctctg atcttctaag
     3001 gaccagtcca cgacgttgac catcatctgc accgtgttgg tatagatggt tccctccgca
     3061 gagcccagca tgcgaaggtt ctggtcggtc aggaagccgt cggaggagaa caagaacagt
     3121 cgggcagagt ccgccgagcg ggctatgacg ctgctcacca ccccgaccgt ggccgccggg
     3181 tcggtctcgt cgtcaccgtc cgcccccgcg tcgttgtcat cgtccccgtc agcaggttcg
     3241 agaaggggcg atgtctggcc ggcaaagaaa gactcgaagc gcccttctat caacgcgccg
     3301 agcagccgcg gcgcctgggc accttccggg gcaaacccgg agagcccctg ctcgtcgagt
     3361 ctcggcatga cgttggtgtc tgccgacagc cagctgcgcg gtgagctggt gagcagctcg
     3421 gtgaccgttc gcccctcctg ctgctcggca tcaatctcga tgggcgaggc ccaggtgagc
     3481 gtaacctgag gaagcccggc cacgatgggc gactcggcat tcattccttc accgcgcacg
     3541 tccacgaagt acgggtagtc gagcatcacc agctcctgaa agctgaaccc gccaacctgt
     3601 cgcgtcaccg gcgccgggaa cgccgcgttc tgagcatcca ttaccagctt gggctcgagc
     3661 gtcaccccca tgtgctcgag ccacgcttcc aggcccgacg tgcgcggcgc cgccatcagg
     3721 ctctgctggg acagagacgc ggaaaagggc gccgtggcga ccaccaccgt gccgcctttc
     3781 atcagaaact gatccatcgc aaagagctgt cgccggttga acccttcagg atccacgatc
     3841 atcaggagat cggcgctgtc gggcacgaca ccctcggcca ggtccgtggt ctccacttcg
     3901 aaatcgctgc gcaggacgtc cttgagctgc tcgtactggt tgccttgggg tggctggccc
     3961 atacccatgt acggcggcgc ttcgggagcc gccagggcca cggtcttcag caacccgctg
     4021 gcaaagcgct tgagcccctc ctcgagcacc cgccgggtcg cttcgaccga gaacgcctcc
     4081 ggcagcggaa gctggatgag cgtgtttgcg tcgcccaggg tcaggtagaa atagaacacg
     4141 ttggaatcga aaaggctggc ggccatgggc tggaaaccga agtccacggc aatctgcgcg
     4201 gccaccgagc catcaccagc gtccgggtcg atgaactccg aagtgaaccg gccgccggac
     4261 tcccgggcaa gcgcatccag ggcctccacg agaacggccc ggtagtcggc caatgcgggt
     4321 ggcagccggt cggcgctgga gacgtagcct acgaagcgca cgggatcctg aatgttggcg
     4381 aagaggctgc cgccgccctg aaagccgtaa aggaccttct tgatggcgcg ggtcagatcg
     4441 aactcggggt ttctaagctg aacgtcgagg tcggcctcgc ccaccacctt gacctcgatg
     4501 agatcgcgga agccgagcac ctcgtactcg tcgccgtagc tcagcaggac gtcgaaatag
     4561 gaattcacca ggctcgattc gtaacggtcc gcgacctgga acggcaccgg ccggattccg
     4621 tatttgctgt tggcctcgtc ctccgcggcg gggtcgtcgg ccggatcgac gatctccaca
     4681 cggatattgc cggcgccggc cacttcaaac tcgttgagca ggtcgcgcat ccggggaacc
     4741 aggggcgcga gcagcggatg cgttcgattg ctgaagtaac cgcgaatcag cagcggttcc
     4801 cgcagctgcc cgatgtacgc ccgggtcgcg tccgagaccg agtagatgcg gccttcggtg
     4861 acgtcgatgc gcaaaccggt cagctgcgcc agccagacgt ttgccagcag caggttggcg
     4921 accagcagcc cggttccgag cagccaacgg cggtgtcggc cggcgccgcc gtcggcagcc
     4981 cagcgctgca cctccagggc atgcacgttc gcggcgagga aaacggccag cagcgaggcg
     5041 tagtagtaga gatcccggag atccaggacg ccgcgggtga tggactcgaa gcgcgagccg
     5101 ctgccgacgc tgcgcaggat gtcggccagc ccgccgccga cgagatccgt gagcaccggg
     5161 ctgccgagca gatagaacgc accacaggca aacgaggcca ggatcaggct gacgatctgg
     5221 ctgtcggtgc gggcgctgac gaagagcccg atggccagat aggcgccgcc gagcagcatg
     5281 gcagccaggt agccggccca gaccgggccc cagtcgagat cggcaatcaa cgagaccgat
     5341 atgggcagag gcagggtcag cagcagggcg atggcaagca ggacccagca cgccaggaac
     5401 ttccccacca cgaactccca gccgctcacg ggcaccgtgg cgacaaactc cagcgtcccc
     5461 gtccggcgct cttcgctcca catgcgcatc gtcagcgccg cagaaaggaa aaccagcagc
     5521 acgggcatcc actcgaacat cggccgcacg tcggcgacgt tgcgggcgaa gaaagcctcc
     5581 acccagaaga acacgaacag cgtgaaggcc aggaaagcac cgagaaacag gtacccgacc
     5641 ggcgaggcaa agaagagcgt cagctccttg ccggcgatcc gcttgacgac atcaggccgc
     5701 atcgctcacc ccctcgttga cctcccggaa cacggtctcc aagtcccgct gcaccgggcc
     5761 gagctcgtga agcgcgatgc cggcgtccac cagcgcccgg gccagacgct ccgccgcggc
     5821 atccagcggc ccgtccacgg tcagctgcca ggccccttcc gccaccgatt ccgcgcggct
     5881 caccccggaa acgccggcga gcagatcggc cacagggccg ccgccggcgc aacgcaggcg
     5941 cagctgatcg ctgcgcttca agtcggccag gcgctcatcg aggaccaggc ggccgtcgcg
     6001 caggatcagc gctcgatcac agatcgcgtc cacctcctgc atgatgtgcg tcgagagcac
     6061 caccgtcgcg gtggccgcca gccgctggat cagctcgcgc atgtgctggg tctggctggg
     6121 atcgagcccg ttggtgggct catcgagaat cacgaacctc ggttcgtgaa gaatggcctg
     6181 agccaccccg acgcgctgct tgaagccgcg cgaaagcgtg ctgat
//
LOCUS       DBNHOX010000436         2633 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1127872, whole genome shotgun sequence.
ACCESSION   DBNHOX010000436 DBNHOX010000000
VERSION     DBNHOX010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1127872"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /gene="odhB"
                     /locus_tag="AC16UZ_09705"
     CDS             <1..877
                     /gene="odhB"
                     /locus_tag="AC16UZ_09705"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HIR6246999.1"
                     /translation="PAARKLATESGLDIQRIEGTGKAGRITREDVAQAASARPEPPAP
                     TPAAVDEASVDEGERVVRRVPMTRLRASIARRLVEAQQNAAMLTTFNEVDMGPIMTMR
                     RRYQDEFQARHNGTRLGFMSFFVRASVEALKRFPAVNASIDENDILYHGYYDIGVAVS
                     TERGLVVPVIREADALSLAQIEDQVNDFAARAQDGKLSIEDMSGGTFTISNGGVFGSL
                     LSTPILNPPQTAILGMHKIQDRPVAVDGEVVVRPMMYLALSYDHRLIDGQEAVRFLVT
                     IKGLIEDPARILLEL"
     gene            880..2319
                     /gene="lpdA"
                     /locus_tag="AC16UZ_09710"
     CDS             880..2319
                     /gene="lpdA"
                     /locus_tag="AC16UZ_09710"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR6247000.1"
                     /translation="MNDKYDVIVIGAGPAGYHAAIRAAQLGLSTACIDKSLDADSNPA
                     YGGTCLNWGCIPSKALLDASHKFVEAKEHFAEIGIRTGELSVDVGQMMARKRQVVQGL
                     TGGVAALFKSNGVTDLPGRGRLLASRQVEYTPHDGDAVVLQAEHVILAPGSVPMEIPP
                     APLTKDLIVDSTGALEFEEVPERLGVIGAGVIGLELGSVWSRLGSEVVVLEALDEFLP
                     MTDERIARDALKIFRNQGLDIRLGTRVMGAEVTDGEVTVTYQDQDGDQSLKVDKLIVA
                     VGRRPFTEGLLAPDCGVNLDERGFLYVNDLCATDAPHVYAVGDVVRGPMLAHKGMEEG
                     VMVAERIAGFKPLVNYECVPAVIYTHPEVAWVGKTEQELKASGDEYNVGSFPFTASGR
                     AMAANDVQGQVKVLADAETDRILGVHVLGPQASELIAQAVIAMEFDACAEDLGLTMFA
                     HPTLSEAVHEAALSVAGHAIHTVNRKKQK"
     gene            2332..>2633
                     /locus_tag="AC16UZ_09715"
     CDS             2332..>2633
                     /locus_tag="AC16UZ_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR6247001.1"
                     /translation="MNLHEYQAKGLFADYGLPVSQGYAVDTADEAVAAAERIGGGRWV
                     VKVQVHAGGRGKAGGVKLADSLDEVRAFADQWIGKNLVTFQTDEHGQPVSKIYVES"
BASE COUNT          496 a          781 c          892 g          464 t
ORIGIN      
        1 cccggcagcc aggaagctgg ccacggagag cggcctggac atccagcgaa tcgaaggcac
       61 gggcaaggct ggccggatca cgcgcgaaga cgtggcccag gccgcctcgg cccggcccga
      121 gccgccagca ccgacgccgg cagcagtcga cgaggcttcc gtcgacgagg gtgaacgtgt
      181 ggtgcgccgt gtgccgatga cgcgcctgcg cgcgagcatc gcccgccggc tggtcgaggc
      241 ccagcagaat gctgccatgc tgaccacgtt caacgaggtc gacatggggc ccatcatgac
      301 catgcggcgt cgctatcagg acgaatttca ggcgcgtcac aacgggaccc gcctcgggtt
      361 catgtctttc ttcgtgcgcg ccagcgttga agccctcaag cgcttccccg ccgtgaacgc
      421 ttccatcgat gaaaacgata tcctgtatca cggctactac gacatcggcg tagcggtgag
      481 caccgaacgc gggctcgtcg tgccggtaat ccgtgaagcc gacgcgctca gccttgcgca
      541 gatcgaagat caggtgaatg atttcgccgc gcgcgcgcag gacgggaagc tttccataga
      601 ggacatgtcg gggggtacgt tcaccatctc caatggcggg gtttttgggt cgctgctgtc
      661 gacgccgatt ctcaacccgc cgcagacggc cattctcggc atgcacaaaa ttcaggatcg
      721 cccggtggcg gtggacggtg aggtcgtggt cagaccgatg atgtacctcg cgctgtctta
      781 cgatcaccgg ctcatcgacg gtcaagaggc cgtgcgattc ctggttacca tcaagggcct
      841 gattgaagac cccgctagaa ttctgctgga actttaacaa tgaatgacaa gtacgacgtg
      901 atcgtcatcg gcgctggccc tgcgggctac catgccgcca tccgcgcagc gcagctgggg
      961 ctcagcaccg cctgcatcga caaatccctg gatgccgaca gcaaccccgc gtacgggggc
     1021 acctgcctga actggggttg tatcccgtcc aaggcattgc tcgatgcctc ccacaagttc
     1081 gtcgaggcca aggagcattt cgctgagatc ggcattcgaa ccggcgagtt gagcgtggat
     1141 gtcggtcaga tgatggcccg caagcggcag gtggtacagg ggctcaccgg tggcgttgcg
     1201 gcgctgttca aaagcaacgg cgtcaccgac ctgcctggaa gggggcgcct cctggccagc
     1261 cggcaggtgg agtacacgcc ccatgatggc gacgccgtgg tgctgcaggc cgagcacgtg
     1321 atccttgcgc cgggttccgt gcccatggag atcccgccgg cgccgctgac caaggatctg
     1381 atcgtcgact cgaccggtgc tctcgagttc gaagaggtgc ctgagcgact gggcgtgatc
     1441 ggtgccgggg tgatcggtct ggagctcggc agcgtctgga gtcgcctcgg ctccgaggtg
     1501 gtggttctgg aagcgctgga tgagtttctt cccatgaccg acgagcgaat tgcccgggat
     1561 gcgctgaaga tctttcgcaa tcaggggctc gatatccgcc tggggacccg ggtaatgggg
     1621 gcggaggtca ccgatggtga ggtaaccgtg acgtatcagg atcaggatgg ggatcagtcg
     1681 ctcaaggtgg acaagctcat cgtggcggtg ggcaggcggc ccttcaccga agggctcctc
     1741 gcgcccgatt gcggggtcaa tctggacgag cgcggcttcc tttacgtcaa cgatctctgc
     1801 gctaccgatg ccccccacgt ctacgccgtg ggcgacgtgg ttcgtggccc gatgctcgcg
     1861 cacaagggca tggaggaggg cgtcatggtc gccgagcgca tcgccggctt caaacccctc
     1921 gtcaactacg agtgcgtacc ggcggtgatc tacacacacc ccgaggttgc ctgggtgggc
     1981 aagaccgagc aggagctcaa agccagcggg gatgagtaca acgtgggcag tttcccgttc
     2041 acggccagtg gccgggcaat ggcagccaac gacgtgcagg gccaggtcaa ggtgcttgct
     2101 gacgccgaga ctgaccggat tctgggcgtg cacgtgctgg ggcctcaggc ttcggagctc
     2161 attgcccagg cagtcatcgc catggagttc gacgcgtgcg cggaggatct ggggcttacg
     2221 atgttcgctc atccgacatt gagcgaagcc gtccacgaag ccgcgctgag cgtcgccgga
     2281 catgccatac atacggtcaa tcgtaagaaa cagaaataga acggaagtct tatgaatcta
     2341 catgagtatc aggccaaggg gctgtttgcc gactatgggc tgccggtgtc gcaggggtat
     2401 gcggtagaca cggcggacga agccgtggcc gccgccgagc ggatcggcgg ggggcgttgg
     2461 gtggtcaagg tgcaggtgca cgcaggcggt cgcggcaagg cgggtggcgt caagctggca
     2521 gacagcctcg acgaggtccg cgccttcgcc gatcagtgga tcggcaagaa cctggtgact
     2581 ttccagaccg atgagcacgg ccaacccgtc agcaagatct acgtggaaag ctg
//
LOCUS       DBNHOX010000437         3188 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1513393, whole genome shotgun sequence.
ACCESSION   DBNHOX010000437 DBNHOX010000000
VERSION     DBNHOX010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1513393"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="AC16UZ_09720"
     CDS             complement(<1..1158)
                     /locus_tag="AC16UZ_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6247002.1"
                     /translation="MHPGIIAAANPDKVAYLMADSGETITYGELEKTSNQGAHLFRQL
                     GLKRGDHIALLLENHPRFFQICWAAQRAGLYYTAISWRLQQAEVEYIVNNCEAAVFIT
                     SAARREVVAPLADSMPGVSHRFVIDGAIEGFDDWAEAISAMPVTPVADESEGMAMLYS
                     SGTTGYPKGVKRPLPDGEYGAAAGINLLNILYGADDDSIYLSPAPLYHAAPLGFTMGC
                     LRYGIQVVVMEHFEAELALRYIQDYRITHSQWVPTMFVRMLKLPEEIRLRFDTSTLRC
                     AIHAAAPCPVPVKEQMIEWWGPVIYEYYAGTEGNGFVQLNATEWLEHKGSVGRPLNCE
                     IHICGDDGEELPVGESGTIYFGGGGEFEYHGDPQKTAGSRHPKGWSTLGDVG"
     gene            1301..2812
                     /locus_tag="AC16UZ_09725"
     CDS             1301..2812
                     /locus_tag="AC16UZ_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR6247003.1"
                     /translation="MASSQPLAVQAGLDAMRSGGNAVDAALATAIALTVVEPNNNGVG
                     SDAFSIVWDGSKLVGLNASGRSPAGWTPDRFAGQRTMPELGWDAVTVPGAVSAWSALS
                     ERYGRLAFPDLFDAAIRYAAEGFPVGPKTAYYWQFAERRYADFPDFANHFLPAPRAGE
                     RFRRPDLARTLQEIAATGGKAFYEGDIAERIETAAAIGGGAMTRDDLAAHRAEWVDPL
                     SQPYRSVDVHEIPPNGQGLAAQIALGIFAHFDAPPLETAESVHLQVEAMKIAIRAAAD
                     HFADADAMRLPPAELLDPANLRRAAGTIGRSASTLPPVSLPTSQDTVYLCAADASGMM
                     VSFIQSNFMGFGSGIVIPGTGISMQNRGSGFTLEPGHPNRVGPRKRPYHTIIPGFVTE
                     NGRPRLAFGVMGGHMQHQGHFQMVQRIFDHGQNPQAASDAPRWRVFPDYSLRVEPEFP
                     EKVIKELADRGHQIGVEDQEHQFGGAQLIYRNESEDAYIAGSDHRKEGMAAGF"
     gene            complement(2818..>3188)
                     /gene="msrB"
                     /locus_tag="AC16UZ_09730"
     CDS             complement(2818..>3188)
                     /gene="msrB"
                     /locus_tag="AC16UZ_09730"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR6247004.1"
                     /translation="ELDTHTYEIARKAGTERAFTGKYWDTKTPGIYHCTCCGASLFDS
                     DTKYDSGSGWPSFYEPVSHDAVSTREDNSLMMQRIEVLCAECDAHLGHLFPDGPEPTG
                     LRFCMNSASLDLQERDEDGS"
BASE COUNT          568 a         1077 c          983 g          560 t
ORIGIN      
        1 cccgacatcg cccagcgtgc tccacccctt gggatgacgg gagccggccg tcttctgcgg
       61 atctccgtgg tactcgaact cgccgccgcc gccgaagtag atcgtgcccg actcgccgac
      121 gggcagctct tcgccgtcgt cgccgcaaat gtggatctcg cagttgagcg gacgtccgac
      181 ggatcccttg tgctccagcc actcggtagc gttcagctgc acgaagccgt taccctccgt
      241 gccggcgtag tactcgtaga tgaccggacc ccaccactcg atcatctgct ccttgaccgg
      301 caccgggcag ggcgcggcgg cgtggatcgc gcaccgcagc gtcgacgtgt cgaagcgcag
      361 ccggatctcc tcgggcagct tgagcatgcg cacgaacatg gtcggcaccc actggctgtg
      421 ggtgattcgg tagtcctgga tgtaacggag cgcaagttcc gcctcgaaat gctccatcac
      481 gacgacctga atgccgtagc gcaggcatcc catggtgaag ccgagcgggg ccgcgtgata
      541 caggggtgcc ggcgaaagat atatggaatc atcgtccgcg ccgtagagaa tgttcagcag
      601 gttgatacca gccgccgccc catactctcc gtccggaagg ggtcttttta cccctttcgg
      661 gtaacccgtc gtccccgagg agtagagcat cgccatccct tcgctttcgt cggcaacggg
      721 ggtgaccggc atggcggaga tcgcctcggc ccagtcgtcg aaaccctcga tggctccgtc
      781 gatgacgaac cgatgggaga cacccggcat actgtccgcc agcggcgcaa ccacctcgcg
      841 ccgtgcagcc gaggtgatga acacggccgc ctcgcaattg ttgacgatgt attccacttc
      901 cgcctgctgc aggcgccagc ttatggccgt gtagtaaagg cccgcacgct gggccgccca
      961 gcagatctga aagaaccgcg ggtggttttc cagcagcaac gcgatgtgat cgcccctttt
     1021 cagcccgagc tgtcgaaaca ggtgcgcgcc ctgattcgaa gtcttctcca gctcgccgta
     1081 agtgatggtc tcgcccgagt cggccatgag gtaggcaacc ttgtctgggt tggccgccgc
     1141 gatgattccc ggatgcacgc tgtctccctt ggattgcgcc cgatatgcag gcagccaaag
     1201 ttaggggctt ttcccgattc acgcaacgca gtaacgtctg ctgatggatt gggaacagcc
     1261 gtatcgggcc gctcgctcgg ccgtttgcgc cgagaacatc gtggccagct ctcaaccgct
     1321 ggccgtgcag gccggcctcg acgccatgcg cagcggcggc aacgcggtgg acgcggccct
     1381 ggcaaccgcc atcgcgctga ccgtggtgga acccaacaac aacggcgtag gcagcgacgc
     1441 gttctccatc gtgtgggacg gttcaaaact cgtcggcctg aacgcttcgg gccgctctcc
     1501 cgccggctgg acgcccgacc gcttcgccgg ccagcgcaca atgcccgagc tcggatggga
     1561 cgccgtgacg gtcccgggcg cggtcagcgc ctggtcggcc ctgtcggagc gctacggccg
     1621 gctcgcgttt cccgatctgt tcgacgccgc catccgctat gccgcggaag ggtttcccgt
     1681 tggccccaaa acggcctact actggcagtt tgccgagcgc cgctacgcgg actttccgga
     1741 tttcgccaat cacttcctgc ccgcgcctcg cgccggtgag cggtttcggc gcccggatct
     1801 cgccagaacg cttcaggaaa tcgcagccac gggcgggaaa gccttctacg aaggcgatat
     1861 cgctgagcga atcgagaccg cggccgccat cggcggtggg gccatgaccc gggatgacct
     1921 ggctgcgcat cgcgccgagt gggtggaccc gctctcccag ccttaccgca gcgtcgacgt
     1981 ccacgagatt ccacccaacg gccagggcct tgccgctcag atcgccctgg gcatctttgc
     2041 ccatttcgac gcgcccccgc tcgaaacggc cgaaagcgtc cacctccagg tcgaagccat
     2101 gaagattgcg atccgggcgg cggccgatca ctttgccgac gccgacgcca tgcgcctgcc
     2161 gccggcagaa ctgctggacc cggcaaatct gcgccgcgcc gccgggacca tcggcaggag
     2221 cgcgtcgacg ctcccgcccg tgtcgctccc aaccagccag gataccgtct acctctgcgc
     2281 cgccgatgcg agcggcatga tggtctcgtt catccaatcc aacttcatgg ggttcggatc
     2341 cggcatcgtg attcccggca ccggcatttc catgcagaac cgcggatcag ggttcaccct
     2401 ggaacccggg cacccgaacc gtgtaggccc ccgaaaacgc ccgtaccaca ccatcatccc
     2461 gggattcgtg accgaaaacg ggcgcccccg gctggcgttc ggcgtcatgg gcggccacat
     2521 gcagcatcag ggacacttcc agatggtgca gcgaatattc gatcacggtc aaaaccccca
     2581 ggccgccagc gacgctcccc gctggcgggt ctttcccgac tacagcctgc gggtggagcc
     2641 ggaattcccg gagaaagtca tcaaagagct ggccgaccgc ggccatcaga tcggtgtcga
     2701 agatcaagaa caccagttcg gcggtgctca gctcatctac cgaaacgagt cggaagacgc
     2761 ctacattgcg gggtcagacc atcgcaagga aggcatggcc gccggcttct gacgaggtca
     2821 cgacccgtcc tcgtcgcgtt cctgaagatc gagcgaggcg gagttcatgc agaagcgcaa
     2881 tccggtcggc tcgggaccgt ccggaaatag gtgacccagg tgggcgtcgc attcggcaca
     2941 gagcacctcg atgcgctgca tcatcaggct gttgtcctcg cgcgtgctca ccgcatcgtg
     3001 ggagacgggt tcgtaaaagc tgggccaacc cgagccagag tcgtacttgg tgtcggaatc
     3061 gaacagagag gcgccgcagc aggtgcaatg atagataccc ggcgtcttgg tatcccagta
     3121 ctttccggta aacgcccgtt cggtgccggc cttccgggcg atctcgtacg tgtgcgtgtc
     3181 cagctccg
//
LOCUS       DBNHOX010000438         9090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1641494, whole genome shotgun sequence.
ACCESSION   DBNHOX010000438 DBNHOX010000000
VERSION     DBNHOX010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9090
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1641494"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="AC16UZ_09735"
     CDS             <1..259
                     /locus_tag="AC16UZ_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR6247005.1"
                     /translation="EAPEALAATRVHHQFPPDDLITYDPSGALPTEAVAGLEALGYRV
                     EPHSWAFGNVQLIWRNREGVLQAASDERFSGASEVLRTPPP"
     gene            complement(272..2623)
                     /locus_tag="AC16UZ_09740"
     CDS             complement(272..2623)
                     /locus_tag="AC16UZ_09740"
                     /inference="COORDINATES: protein motif:HMM:NF017197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HIR6247006.1"
                     /translation="MSRKIWIPAVIALLLISIGLAAPLAIKRYVEGWLRDRGVAEARI
                     TDVDLNPFTGRVRVEYLDAPGAADESGSFEMLELDLELLGLVTGRVMVSRAILRDAVL
                     DVRRMPDGRFSVGGLMFPLAQAEQEVESDGEGPAGSPRPRLGLAGLLVENVTLTYDDG
                     MLSDRLYIRSLTIDRMVTWEPERAARLVAELELNGAPVGLEAEVFAFADERRATISID
                     VDAFDVAAFAPLVADLGIESPGGSLSLSLGGQLRREASGRLFGNVAGTVGVDDIAFGL
                     HPLQIDLGKLSLDVDLAGEIEIQSGDSPPAISGQIGVAASDLLVDHRDWPARLLGLET
                     MEAPSVRVDGLERASSDTLSVRGLRLLEEEEVVGLSVGSASVEGIELDTSARALRIGE
                     VSLDDLVARLRRGEQGRLMLLGPAAAEEPSAQPEAAPGADDGAPSFELHVGAVSIGGN
                     SALGLEDLSVEPQVTMELVIDRFRLADLGIGPAAGPMVLDVALTQNGAQLSATGDLAL
                     FADARQVKADLEVVHFDLSQLTPYLPNYRVDRGRLSIQTQLGINGDEVAAENAVLLEK
                     LKVGVKRSGDPGLVEEGLGMPLDVALDLLRDGDDRIKFDLPISGSIHDPKFGTGDIVR
                     LAMQNALQRAALSVATNALQPLGTIMFVGKMAGKAARPRFDPVAFEPGARSLSELGTS
                     YLDKLAGLLLERPRLHLTFCGVAAVQDVAAAEAVEGDGSASADGAPAVVDEAALLELA
                     RARGSAVHDYLIDVRGIPAARLFRCHPEYEAESEDGPRVEIML"
     gene            complement(2649..3857)
                     /locus_tag="AC16UZ_09745"
     CDS             complement(2649..3857)
                     /locus_tag="AC16UZ_09745"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6247007.1"
                     /translation="MKLEDVDPLDPDIVECPYEFYRALRERGVYRVPQRGFFLVGNYD
                     TAMQVIKDTDTFSSKSGVAVPRSPDGSHPSNPREVRTLLTADPPEHRKYRELVNKAFS
                     AKRVASWEPRIREIADELIEGFAGTGTCELVHDFAVPLPLIVICEAMGLPREMLRTFK
                     RWSDGIASLGGMITPEQRRQIAAEQADFAAYVTEMIEERRSNLGDDFVSDLIRARFDG
                     ARPLNIDELTSLVIQFLVAGNETTTNTIASGMWLLLTHPEQLQAAREDPGLIPNLVEE
                     VLRIESPVQAHFRAAARDTELDGVPIPAGAGVGVIFGCCNRDDDKFADAAEFDVRRDN
                     ASRHLAFSQGIHFCPGAPLARLESAIAFERLLERLGNLRLEGDPNDLAHVPSFTHRGL
                     KKLVVRFDPT"
     gene            complement(4021..5181)
                     /locus_tag="AC16UZ_09750"
     CDS             complement(4021..5181)
                     /locus_tag="AC16UZ_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247008.1"
                     /translation="MKRIPKWLSGGGLAALLLVLGGQAFGKACADLIAGQHTLVGTVC
                     VGVADDSVQVEYTVIAGWSINSVHFFFDDKPTEEELSVNSCPLGPNFPMTDPKDPYDC
                     GNPKLGHFTYNTSGLNTSTYVFSLPLNGIDYLEHGDLCGSEFNIVAHADVTGPEGQTE
                     GAWAVSWGEMEIDGQMVSFPNYILVDPEDPHERPRGNWGFAGMFDMKVNCEGSFKCET
                     AFAKGDYTFETWPHLDFNSNRWGWLLGPFGKGFVANSESFPLYAGAGQNDISKGALAG
                     VVTVSWDTAYEANQVDFKHHNVAVSYNMNPEWFISSTAENDPTQLLPGSVHIFIGAEG
                     ELLTTAPGQYTYVLDQSEAEVYAYSMSPVTVDAEEFWVVAHAEVCSFVSGEE"
     gene            complement(5424..6731)
                     /locus_tag="AC16UZ_09755"
     CDS             complement(5424..6731)
                     /locus_tag="AC16UZ_09755"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6247009.1"
                     /translation="MEIRRKQVIERIDPKGQNVGSPADKAPDQDLGHELIPKQRYTSA
                     DFMRREWEGIWSRVWLLGCREEQIAEPGDFICTNIGKESVLLVRQADGGVRAFYNVCM
                     HRGNRLAEEGAGHAETFRCSYHDWQYGLDGRFVDIPDLNTFPQGAPPCAGLREIPCDT
                     WASFVWFSLNEDVEPLKDYMGDLVGHLEPYHFERMAMTRWVTAEWNCNWKASVDAFSE
                     SYHTARTHPQLLWYLDDYNVQIDLYDKHSRYLIPFGLLSPRVDSAPEIPPPLKVMLKN
                     AGIDPASYDGPVGGIRKAVQQHMRESQAALGKDYSELNDDQLTDDYNYLIFPNVTCNT
                     HADNLMLFRNRPHPEDPNRMLFDIWMFELLPEGEERPALPRHDFRPEGSTRSLGLVID
                     QDASNLASVQAGMNSAAYPGLWLSDQELRLRHFHKTISDFVGD"
     gene            complement(6745..7506)
                     /locus_tag="AC16UZ_09760"
     CDS             complement(6745..7506)
                     /locus_tag="AC16UZ_09760"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6247010.1"
                     /translation="MDLGLAGKRAIVPGSTKGILRRVVDQLVDEGCDVAICSRSEDTV
                     ARALRELSGGRGKAIGRACDVMDKEDYEAWIAEMAESMGGADIFIPGVSAGGGSDSER
                     NWWKNFEVDVLSTVRGCEAVLPFMRQSGGGAITFVTTTASVETFGGPQAYNALKGSLL
                     VYAKQLSQAVGRDKIRVNCVSPGPIYFEGGVWAMLEDTMAKWFEKTVRDHPQGRLGAP
                     EEVANCILFLSSPAASWVSGTNLIVDGGFTKRVQF"
     gene            complement(7518..8708)
                     /locus_tag="AC16UZ_09765"
     CDS             complement(7518..8708)
                     /locus_tag="AC16UZ_09765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6247011.1"
                     /translation="MTNDVYLAAGTRSALGNFGGSLKDVPMTELASHVARACIERSGA
                     NPDSVDHMVFTTTCPTDRDSLFSARVVGVKSGIPEMASALDVSRACASGLQAILSAGQ
                     QIESGQSRLALAGGSEMFSRVPYAVTTARWGIVRGNQQLEDMLEWCYRCPFSLEYMGE
                     TAENLTEEFQYDREAMDEYAVSSQARALAAIESGFLARQIVPVDVPDGRGTRTFEVDE
                     SPREGIDMERLARLKPAFREGGKVTAGNSSGVTDGAAAILVGSRGALESESVEPEARV
                     VDWAVVGVPPRIMGHGPVPAIQLLLERTGLSISDIDYFEVNEAFAVVNLHVERHLGIP
                     RDAHNLYGGGISVGHPPGVTGLRMAINAIQHLRETGGRYAILSMCMGGGQGMAMLIEN
                     AARH"
     gene            8837..>9090
                     /locus_tag="AC16UZ_09770"
     CDS             8837..>9090
                     /locus_tag="AC16UZ_09770"
                     /inference="COORDINATES: protein motif:HMM:NF015441.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HIR6247012.1"
                     /translation="MTVIGVVKEIWRFPVKSMQGSTIETCTISRTGIPGDRGWAMRDE
                     ERQEIQWGKRHPELMLCNASYRGEPEPGQTTPVDVTFPDG"
BASE COUNT         1641 a         3011 c         2871 g         1567 t
ORIGIN      
        1 ggaggcgccg gaagcgttgg cagcaacccg cgtgcaccac cagttccctc ccgacgatct
       61 gataacgtac gacccgtcgg gcgccctgcc gaccgaagcc gtcgcgggcc ttgaggcgct
      121 cggctatcgg gttgagcccc actcctgggc gttcggcaac gttcagctca tctggcgcaa
      181 ccgcgagggc gtgctgcagg cggcatcgga cgagcgcttc tcgggcgcga gcgaggtgct
      241 gcgaactcca ccgccttgac gctcacgcag ctcagagcat gatttccacc cgcggcccgt
      301 cctcggattc ggcctcgtat tccgggtgac agcgaaacag gcgcgcggcc ggtatgccgc
      361 gcacgtcgat gaggtagtcg tgcacggccg agccccgggc cctggcaagc tcgagcaggg
      421 ctgcctcatc aacgacggcc ggggcaccat ccgcgctcgc gctgccgtcc ccctccaccg
      481 cttccgccgc tgccacatcc tggacagcgg cgacgccaca gaacgtcagg tgcagacggg
      541 gccgttccag cagcaggccg gcgagcttgt ccagatagct cgtgcccagc tcgctgagcg
      601 accgcgcgcc gggctcgaaa gccaccgggt cgaaacgggg ccgtgccgcc tttcccgcca
      661 tcttgccaac gaacatgata gtgcccagcg gctgcagcgc gttcgttgcg acgctgagcg
      721 ccgcgcgctg cagcgcgttc tgcatggcca gccgcacgat gtcgccagtg ccgaatttcg
      781 gatcgtggat gctgccggag atgggcagat cgaacttgat ccgatcgtcc ccgtcccgaa
      841 gcagatccag agcaacgtcc agcggcatgc cgagaccctc ttccacgagc cccggatcgc
      901 ccgaccgttt gacgcccacc ttcagcttct ccagaagcac ggcgttctcg gctgcgacct
      961 cgtcgccgtt gattcccagc tgggtctgga tcgaaaggcg gccccggtcc acccggtagt
     1021 ttggcaggta gggggtcaac tggctgagat cgaaatggac gacttccagg tctgccttca
     1081 cctgccgggc gtcagcgaac aacgccagat ccccggtagc ggagagctgc gcgccattct
     1141 gggtgagcgc gacgtccagg accatgggcc cggcagccgg gccgataccc agatcggcaa
     1201 gccgaaatct gtcgatgacg agctccatgg tcacctgcgg ctcgacggat aagtcctcca
     1261 aacccagggc gctgtttccg ccgatgctca ctgcccccac atgaagctcg aacgacggcg
     1321 cgccgtcatc ggcacccggc gccgcttccg gctgagcaga cggctcctcg gcggcagccg
     1381 gcccaagcag catgagccgg ccttgctctc cacgccgtaa ccgggccacg aggtcgtcca
     1441 gggagacctc cccgattctc aacgcgcgag cgctggtatc gagttcgatg ccctccacgg
     1501 aggccgagcc caccgacagc ccgacgacct cttcctcttc caaaagcctg aggccgcgga
     1561 ccgagagggt gtcagacgac gcgcgctcga gcccgtcgac gcgcaccgac ggcgcctcca
     1621 tggtctccaa acccagcaac cgggcaggcc agtccctatg gtctacgagg aggtcgctcg
     1681 cggccacgcc gatctgcccg gagatcgcag ggggcgagtc acccgattga atttcgatct
     1741 ccccggccag atcgacgtcg agactcagct tacccagatc gatctgcagg ggatgcaaac
     1801 cgaaggctat gtcatcaacc cccaccgtcc cggccacgtt gccgaacagc cgcccggaag
     1861 cctctctgcg cagctgcccg cccaaagaaa gcgatagcga tccgcccggc gactcgatac
     1921 ctagatcggc caccagcgga gcaaacgcgg caacatcgaa agcatccacg tcgatcgata
     1981 tggtggcgcg ccgctcgtct gcgaacgcga acacctccgc ctccagccca accggcgccc
     2041 cgttcagctc gagctcggca accagccgag cggccctctc gggttcccag gtgaccatgc
     2101 gatcgatggt cagagaacgg atgtagagcc gatcgctgag catcccgtcg tcgtaggtaa
     2161 gggtcacgtt ctccaccagg agtccggcta agccgagcct gggacgcggt gatccggcgg
     2221 gaccctcgcc gtcgctctcc acttcctgtt ccgcctgggc cagggggaac atcaggccac
     2281 ccacggagaa gcgcccgtcc ggcatccgcc gcacgtccag gacggcatcc cgcaggatgg
     2341 cccgcgaaac catcacccgc ccggtaacga ggccgagcag ctccagatcc agctccagca
     2401 tctcgaagct gcccgactca tcggcggcgc cgggagcatc cagatactcg acccgtaccc
     2461 tgccggtgaa ggggttcaag tccacgtcgg tgatacgggc ctcggcaacg ccgcgatcac
     2521 gcagccagcc ttccacgtag cgcttgatgg ccaggggcgc ggccagcccg atgctgatca
     2581 gcagcagggc aataacggcc ggaatccaga tctttcggct cacgctgacg ggcaacgtct
     2641 gcatctcatc atgtcggatc gaagcgaact accagcttct tcaggccacg atgggtgaag
     2701 ctggggacgt gggcaagatc attcggatcc ccttcgagac gcaggttgcc gagccgttcg
     2761 aggaggcgct cgaaggcaat cgcgctttcc agacgggcca gcggcgcgcc cgggcagaag
     2821 tgaatgccct ggctgaaggc gaggtgacgc gaggcgttat cgcgccggac gtcgaattcc
     2881 gccgcgtcgg caaacttgtc atcgtcccga ttgcagcagc cgaagatgac gcccaccccg
     2941 gctcccgcgg ggatcggcac gccgtcgagc tccgtgtccc gggcggcagc tctgaagtgc
     3001 gcctgcacgg gcgactcgat tctcagcact tcctcgacga ggttggggat caggccgggg
     3061 tcctcgcgcg cggcctggag ctgctcagga tgagtgagca ggagccacat cccggacgcg
     3121 atggtgttgg tggtggtctc gttacctgcc accagaaact ggatgacgag gctcgtaagc
     3181 tcatcaatgt tcagcggccg tgcgccgtcg aagcgggctc ggatcagatc ggagacgaag
     3241 tcgtcgccga gattcgatct gcgctcctcg atcatctcgg tgacgtatgc ggcgaaatcc
     3301 gcctgctccg cggcgatctg ccggcgttgc tccggcgtga tcatgccacc gaggctcgcg
     3361 atcccgtccg accagcgctt gaaggtgcga agcatttccc ggggcaggcc catggcctcg
     3421 cagatgacga tgagcggcag gggcacggca aagtcgtgga cgagctcgca ggtgccggtg
     3481 ccggcaaagc cttcgatcag ctcgtcggcg atctcccgga tgcgcggttc ccaggaggcc
     3541 acccgcttgg cggagaaggc cttgttgacc agctcgcggt acttgcggtg ctcggggggg
     3601 tccgccgtga gcagcgtgcg cacctcgcgc gggttcgacg ggtggctgcc gtccggggat
     3661 ctcggcaccg ccacgccgga cttcgacgaa aaggtgtccg tatccttgat cacctgcatg
     3721 gcggtgtcgt agttgccgac aaggaagaat ccgcgctgcg gcacccggta gacgccgcgc
     3781 tcccgcagcg cccggtaaaa ctcgtacggg cactcgacga tgtcgggatc cagcggatcg
     3841 acgtcttcaa gcttcatctg cggttcactc ccaccccccg atgcgattct gcgcggagcg
     3901 ctgccggcgc gcaatcggcc cggacgtcag cctcgcccca caagtaaccg agcggccaaa
     3961 aaaagagggt gccgaagcac cctccaatcc gcctgttctt ctgaacagtg tcctgtcgat
     4021 ctactcctcg ccggagacga acgagcaaac ctcagcgtgc gccaccaccc agaattcctc
     4081 ggcatccacc gtcaccgggc tcatgctgta agcataaacc tcggcctcgc tctgatcgag
     4141 gacgtaggtg tactggccag gggccgtcgt gaggagttcc ccctcagcac cgatgaatat
     4201 gtgcacggat ccggggagca gctgggtcgg gtcgttctcc gcggtgctcg agatgaacca
     4261 ttccggattc atgttgtagc tgaccgcgac gttgtgatgc ttgaagtcca cctggttggc
     4321 ctcgtaggcg gtatcccagc tcacggtgac cacaccagcc agagcgcctt tggatatgtc
     4381 gttctgaccg gctcccgcgt agagcgggaa ggactccgag ttggcgacga accctttgcc
     4441 gaacggcccg agcagccagc cccaacgatt gctgttgaag tcaaggtggg gccaggtctc
     4501 aaaagtgtaa tcccctttgg cgaaggctgt ctcgcactta aagctacctt cgcagttgac
     4561 cttcatgtcg aacataccgg caaagcccca gttcccgcgc gggcgctcat gggggtcttc
     4621 aggatccacg aggatgtagt tggggaagct caccatctgc ccatcgatct ccatctctcc
     4681 ccagctgacg gcccaagcgc cttcggtttg gccttcgggg cccgtgacat cggcgtgggc
     4741 aacgatgttg aactcggacc cacagagatc accatgctcg agatagtcga tgccgttcaa
     4801 cggcaggctg aagacgtagg tcgaggtgtt caggccactc gtgttgtagg tgaagtgccc
     4861 gagcttcgga ttgccgcagt cgtaaggatc cttgggatcg gtcatgggaa aattcggacc
     4921 gagcggacac gaattcaccg aaagctcttc ttccgtcggc ttgtcgtcga agaagaaatg
     4981 aacagagttg atcgaccagc cggcgatcac cgtgtactcg acctgcacgc tgtcatcggc
     5041 aacgccgacg caaacggtac cgacgagggt atgctggcct gcaatcaggt cggcacaggc
     5101 cttgccgaag gcctgaccgc ctaacacgag aagcaaagcg gcgaggccgc ctccgctgag
     5161 ccacttcggg attctcttca cgagatgcac ctcctagttg tagagaacgc gcatccccct
     5221 cggcgcgacc ggctcctgtt tctatgtcgg attaccgatc tgaagattct gacgattggt
     5281 gctggcgttg tggtaaagga gtgtgtccgc tttgtccggg tgggccccgg ttacgtaggg
     5341 caaatgcaaa agtgtcgacc agttcacaga actggtcgga cgaccgtatc caagctgaaa
     5401 ggcccgccgt cgcgcggtgc tcgtcagtcg ccgacgaagt cgctgatggt cttgtgaaag
     5461 tggcgcagcc ggagctcctg gtcggagagc cagagcccgg gatacgcggc gctgttcatg
     5521 ccggcctgaa cggacgccag gttcgaggcg tcctgatcga tgacgagccc gagagaccgc
     5581 gtgctgcctt ccggccggaa gtcgtgcctg ggcagcgcag gccgctcttc gccctcgggc
     5641 agcagctcga acatccagat gtcgaatagc atccggttcg gatcctccgg gtggggccgg
     5701 ttgcggaaca gcatcagatt gtcggcgtgg gtattgcagg tgacgttggg gaagatcagg
     5761 tagttgtaat cgtcggtcag ctgatcgtcg ttcagctccg agtagtcctt tccaagggcc
     5821 gcctggctct cccgcatgtg ctgctggacg gccttgcgga tcccgcccac gggaccgtcg
     5881 taggaagcgg gatcgatccc ggcgttcttg agcattacct tcagcggggg cggtatctcc
     5941 ggcgcggagt ccacccgcgg gctcagcagg ccgaacggga tcaggtaccg gctgtgcttg
     6001 tcgtagaggt cgatctgcac gttgtaatcg tccagatacc aaagcagctg ggggtgggtg
     6061 cgcgccgtgt ggtagctctc ggagaaggcg tcgacggacg ccttccagtt gcagttccac
     6121 tccgcggtca cccagcgggt catggccatg cgctcgaagt ggtagggctc gaggtgcccc
     6181 accaggtcgc ccatgtagtc cttcagcggc tcgacgtcct cgttcaggct gaaccagacg
     6241 aagctcgccc aggtgtcgca ggggatctcg cgaagcccgg cacagggcgg ggcgccctgg
     6301 gggaacgtgt tcagatccgg aatgtccacg aagcggccgt ccaggccgta ctgccagtcg
     6361 tgatagctgc agcggaacgt ctcggcatgg ccggctccct cctcggccag ccgattaccg
     6421 cgatgcatgc agacgttgta gaaggcccgg acgccgccat cggcctggcg gaccagaagg
     6481 accgattcct tgccgatgtt cgtgcagatg aagtcgccgg gctcggcgat ctgctcctcc
     6541 cggcagccca gcagccagac cctgctccag atgccctccc actcccgccg catgaaatcg
     6601 gcggacgtgt agcgctgctt gggaatcagc tcgtggccga ggtcctgatc cggggcttta
     6661 tctgcgggcg agccgacgtt ctgtcctttt gggtcgatac gttcgatgac ctgtttgcgg
     6721 cgaatctcca tcagcaggga cgctctagaa ctgcacccgt ttggtgaagc cgccgtcgac
     6781 gatcaggttc gtgccggaca cccagctcgc cgccgggctg gacagaaaca ggatgcagtt
     6841 ggctacctcc tcgggcgcac cgagacgacc ctgggggtga tcgcgcacgg tcttctcgaa
     6901 ccacttggcc atggtgtcct ctagcatggc ccagaccccg ccctcgaaat agatcgggcc
     6961 gggggagacg cagttgacgc gtatcttgtc gcggcccact gcctgtgaga gctgcttggc
     7021 gtagacgagc agggagcctt tcagcgcgtt gtaggcctga gggccgccaa atgtttctac
     7081 tgaagccgtg gtggtaacga acgttatcgc tccgccgccg gactgccgca tgaagggcag
     7141 caccgcctcg cagccgcgaa cggtagagag gacgtccacc tcgaagttct tccaccagtt
     7201 gcgctcgctg tcgctgccgc caccggcgct gacgccagga atgaaaatgt ccgcaccacc
     7261 catggattcc gccatctcgg cgatccaggc ctcgtagtcc tccttgtcca tcacgtcgca
     7321 ggcccggccg atggccttgc ccctgccccc ggacagctcc cgcaaggcac gcgccacggt
     7381 atcttcggag cgcgagcaga tggccacatc gcagccctcg tccacgagct ggtccacgac
     7441 ccgccgcagg atgcccttgg tcgatccggg cacgatggcc cgcttgcctg ccaatcccag
     7501 gtccatcttc gtcgctccta atgccgggcc gcgttctcga tgagcatggc catgccctgg
     7561 ccacctccca tgcacatcga gagaatggcg tagcgcccgc cggtttccct gagatgctga
     7621 atggcgttga tggccatgcg caagccggtg acccccgggg gatggccaac ggagatgcca
     7681 ccgccgtaga ggttgtgagc atccctggga attcccaggt ggcgctccac gtgcaggttc
     7741 accacggcga acgcctcgtt cacctcgaaa tagtcgatgt ccgaaatgct cagcccggtc
     7801 cgttccagca gaagctgaat ggcaggcacc ggtccatgtc ccatgatccg cggcggtacg
     7861 cccaccaccg cccagtcgac gacgcgggct tccggttcca ccgactcgga ctcgagcgcc
     7921 cctcggctgc ccacgaggat ggcggcggca ccgtccgtga cgccggacga gttgccggcc
     7981 gtgaccttcc cgccctcccg gaacgcgggc ttcagacgtg caagacgctc catgtcgatg
     8041 ccctcgcggg gggattcgtc cacctcgaag gtgcgcgtgc cgcgtccgtc gggaacgtcc
     8101 acgggcacga tctgcctggc gagaaagccc gactcgatgg cagccagagc ccgcgcctgg
     8161 ctggacaccg cgtactcatc catggcttcc cggtcgtact gaaattcctc ggtgaggttc
     8221 tcggcggttt cgcccatgta ctcaaggctg aacgggcacc gataacacca ctcgagcatg
     8281 tcctccaact gctggttgcc ccggacgatg ccccagcggg cggtggtcac cgcgtacggc
     8341 acccggctga acatctccga cccgcctgcc agggccagcc ggctctgccc cgactcgatc
     8401 tgctgacccg cggagaggat ggcctgcaga ccggaggcac aggcgcggga gacgtccagg
     8461 gctgacgcca tctcaggtat gccgctcttc acgcctacca cgcgggcaga gaagagcgag
     8521 tcccgatccg tggggcaggt ggtggtgaag accatgtggt caacggaatc cgggttcgcg
     8581 ccggaacgtt cgatgcaggc gcgcgccacg tgggaggcaa gctccgtcat gggcacgtct
     8641 ttgagcgaac cgccgaaatt gcccagcgcc gagcgggtgc cggcggcgag atacacgtcg
     8701 tttgtcatca ctccctcccg tcagggcatt ctaggtcagc gccagagcgc ccgccagaac
     8761 gttcccgaga tccagattgg acgcgactac cccacagtgc gacgattgcg gtgcatccaa
     8821 agaggggaga tctcgagtga ctgtcatcgg cgtggtgaag gagatctggc gcttcccggt
     8881 caaatcgatg caggggagca cgatcgagac gtgcaccata tccaggacgg gcattcccgg
     8941 cgacaggggc tgggcgatgc gcgacgagga gcgccaggaa atccagtggg gcaagcgtca
     9001 tcccgagctc atgctgtgca acgcctccta tcgaggtgaa cccgagcccg ggcagaccac
     9061 tccggtcgac gtcacttttc ccgacggcga
//
LOCUS       DBNHOX010000439         3252 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_163752, whole genome shotgun sequence.
ACCESSION   DBNHOX010000439 DBNHOX010000000
VERSION     DBNHOX010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_163752"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="AC16UZ_09775"
     CDS             complement(<1..736)
                     /locus_tag="AC16UZ_09775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6247013.1"
                     /translation="MKIGLATPDGLWASDLDSRRELLAQVAAAGVDHVFMADHVSFRN
                     GSGTDGFVEIAGLSQLHPTLGVMISIYLLPLRHPVPVARQLATVNRLAPGRVLFGVGI
                     GGEDRHEIEVCGVDPATRGRRMNESLSVLRGLMGGEEVTFHGEFFDLDRALIRPAIEP
                     AIPIIVGGRSDAALVRAGRLGDGWIGAWCSVRRFSQALGIIDDAAEQAGRVAVDWQHG
                     YQPWVGVGDTRDEARGRVAEAMEAFYK"
     gene            complement(756..2228)
                     /locus_tag="AC16UZ_09780"
     CDS             complement(756..2228)
                     /locus_tag="AC16UZ_09780"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6247014.1"
                     /translation="MRVELIESFASGLPEEDDHPYRTGAWQPNVREYDAVDLTVTGRL
                     PDDLSGVYLRNTENPLHDAIGRYHPFDGDGMLHALFVDNGEAEYRNRFIRTDGFAAEL
                     AEGRPLWAGLMESPKKSLRDGCGARTRLKDASSTDVVVHGGRAVTSFYQCGDLYEFDP
                     RSLTQLGKADWVAGTTPGWGISAHTKVDEATGEMLFFNYAKEAPFMHYGVVNAARELV
                     HYVPVPLPGPRLPHDMAFTDNYAILNDFPLYWDPQALRAGAHAVRFFADQPSRFGIIP
                     RRGGSHEVRWFEAKPTFVLHWINAYEDGDEVVLDGFFEENPNPKLTIDPNDKRSMFRM
                     LDMHVIGAKPHRWRFNLRTGATREEYLDDAVSEFGMINPAHGGKPYRYVWAVTLKPGW
                     FLMDGLVRLDVQTGEKQVYQLPDGVFLSESPMAPRVGSTAEDDGYVVTFVTDMNRDAS
                     SAMIFDAADIEQGPVAQVQLPERICVGTHSCWSDKRNFAR"
     gene            complement(2263..3015)
                     /locus_tag="AC16UZ_09785"
     CDS             complement(2263..3015)
                     /locus_tag="AC16UZ_09785"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase-like domain-containing
                     protein"
                     /protein_id="HIR6247015.1"
                     /translation="MHRLLASCAEPQEEISPASPPPTIYAAGTLITMDPGLPRAEAVA
                     VADERILAVGTLAELRGRYPGAAVDETFAERVVMAGLIDQHLHPFLAALTMTAEVVSI
                     EEWVLPARMFPAARDRDAYLCLLGAAAANMEDPAEPLISWGYHHYLHGSLTRKDLDAI
                     SESRPIMVWHRSAHEFVLNTPALAAAGVTEALVAAEEEVIREQIDLADRHFWERGSFD
                     FLVPRLMPMLAAPDRFRAGLAALTGGCEIRWA"
BASE COUNT          549 a         1114 c         1070 g          519 t
ORIGIN      
        1 ccttatagaa cgcttccatg gcctcggcca ctcggccgcg cgcctcatcg cgggtgtccc
       61 ccacgcccac ccagggctgg tagccgtgtt gccagtccac ggcgacccgt ccggcctgct
      121 cagccgcgtc gtcgatgatc ccgagcgcct gggagaagcg tctcaccgag caccacgcac
      181 cgatccagcc atcgccgagg cgaccggccc ggacgagagc ggcatcggag cggccgccca
      241 cgatgatggg aatcgccggc tcgatggcgg gccgaatcag cgcccggtcg agatcgaaga
      301 actcgccatg aaaggtcacc tcctcgccac ccatgaggcc gcgcagcacg ctgaggcttt
      361 cgttcatgcg ccgcccccgt gtggcaggat cgacgccgca aacctcgatc tcatgcctgt
      421 cttcaccccc gatgccgacc ccgaagagta cccggcccgg cgccagacga ttcacggtgg
      481 cgagctggcg cgcaacggga acgggatgcc gcaggggcag caggtaaatg ctgatcatca
      541 cgcccagcgt cggatggagc tgcgaaaggc ccgcgatctc cacgaagccg tcggtgcccg
      601 agccgttgcg gaaagacacg tggtcggcca tgaagacgtg gtcgaccccg gcggccgcga
      661 cctgcgccag cagctcccgg cggctgtcga ggtcggacgc ccataggccg tcaggcgtgg
      721 caagaccgat cttcacgatg gttccggccg cccgttcagc gcgcgaagtt gcgtttgtcg
      781 gaccagcacg agtgggtgcc cacgcagatg cgctcgggca gctggacctg ggcgacggga
      841 ccctgctcga tgtcggcggc atcgaagatc atggcgctgg acgcgtctct gttcatgtcc
      901 gtgacaaagg tcaccacgta gccgtcgtcc tcggccgtcg acccaacccg cggggccatg
      961 gggctttcgc tgagaaaaac gccgtccggc agctggtaga cctgtttctc gccggtctga
     1021 acgtccagcc ggacaaggcc gtccatgaga aaccagcccg gcttcagcgt gacggcccag
     1081 acgtagcggt agggtttacc cccgtgggcc gggttgatca tgccgaactc ggacaccgcg
     1141 tcgtcgagat actcctcccg cgtagcgccg gtgcgcaggt tgaagcgcca gcgatgcggc
     1201 ttggcgccga tcacgtgcat gtccagcatc cggaacatcg agcgcttgtc gttcgggtcg
     1261 atggtcagct tgggattcgg gttctcctcg aagaagccgt cgaggaccac ctcgtcgccg
     1321 tcctcgtagg cgttgatcca atgcagcacg aacgtgggct tcgcctcgaa ccagcgcacc
     1381 tcgtgggaac caccgcggcg cgggatgatg ccgaagcggc tcggctgatc ggcaaagaat
     1441 cgcacggcgt gagcgcccgc tcgcagcgcc tgcgggtccc agtagagagg gaagtcgttg
     1501 aggatcgcgt agttgtcggt gaacgccatg tcgtgtggca ggcgcgggcc gggcaacgga
     1561 accggaacgt agtgcaccag ctcgcgggcg gcgttgacca cgccgtaatg catgaacgga
     1621 gcttccttgg cgtagttgaa aaagagcatc tcgcccgtgg cctcgtccac cttggtgtgg
     1681 gccgatatgc cccagccagg cgtggtgccg gccacccagt cggccttgcc aagctgggtg
     1741 agggagcggg gatcgaactc gtacaggtcg ccgcactggt agaagctggt gaccgcccgg
     1801 ccgccgtgga cgaccacatc cgtgctggaa gcatccttga ggcgcgtgcg cgcgccgcag
     1861 ccatcgcgca gggatttctt gggcgactcc atgagcccgg cccacaaggg ccggccttcg
     1921 gccagctcgg ctgcgaaccc gtcggtgcgg atgaaccggt tgcggtattc ggcctccccg
     1981 ttgtccacga acagcgcgtg cagcatgccg tcaccgtcaa aggggtggta gcgcccgatg
     2041 gcgtcatgca gcgggttctc cgtgttgcgc aggtacacgc ccgacaggtc atcggggagc
     2101 cggccggtga ccgtcaggtc cacggcgtcg tactcgcgaa cgttcggctg ccaggcaccc
     2161 gtccggtagg gatgatcgtc ctcctcggga aggcccgagg cgaaggattc gatcagctcc
     2221 actctcatct gccgctcccc ctttgtctca ccatgcacat attcaagccc atcgtatctc
     2281 acatccgccc gtcagcgccg cgagcccggc gcggaagcga tccggggcgg cgagcatggg
     2341 catcagtcgg ggcaccagaa aatcgaaact gccccgctcc cagaaatgcc tgtccgccag
     2401 gtcgatctgc tcgcgaatca cctcctcctc ggccgcgacc agcgcctcgg tcacccccgc
     2461 tgcagccagg gccggcgtgt tcaggacgaa ctcgtgggcc gaacggtgcc acaccatgat
     2521 gggccgcgac tcgctgatgg cgtcgaggtc ctttcgggtg aggcttccgt ggaggtagtg
     2581 gtggtatccc cacgagatca gcggctcggc cggatcctcc atgttcgccg ccgcggcgcc
     2641 cagcaggcaa aggtacgcat cacgatcccg ggccgcagga aacatccggg ccgggagaac
     2701 ccattcctcg atggagacca cctccgccgt catggtcaac gccgccagaa acgggtgcag
     2761 gtgctgatct atcaggccgg ccatgaccac gcgctcggca aaggtttcat ctaccgcggc
     2821 cccgggatag cggccgcgca gctccgccag cgtccccacg gcgaggatgc gctcatcggc
     2881 aacggccacc gcttcggccc gcggcagccc agggtccatg gtgatcagcg tgcccgccgc
     2941 gtagatcgtc ggcggcgggc tcgcgggcga gatttcttcc tgcggctccg cgcaggacgc
     3001 gagcaatcgg tgcaaacgcc tcgttgatgg tctcgtcgat cctcatggcg cgagcataag
     3061 ccggagccgt cggcgcttgt ccagtgcgac gccgcgccgt ggcgcgccgc caccgcgcat
     3121 cagaaccccg tggactgcca ggccagcatg gcgaaggcgc cgttcgcccg acgccacacg
     3181 ctcaagaact tgttgcgcag cgccttctcc gcgccatccc tgcttgcccg gccgttgagg
     3241 atgccgtggc ag
//
LOCUS       DBNHOX010000440         1723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1705897, whole genome shotgun sequence.
ACCESSION   DBNHOX010000440 DBNHOX010000000
VERSION     DBNHOX010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1705897"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="AC16UZ_09790"
     CDS             <1..635
                     /locus_tag="AC16UZ_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247016.1"
                     /translation="PFVAVARFATFQSAFQFRSVDPQTQEEATFQGRSFGRYRPIHSA
                     WDINDGQRGYQPTAGLAFPGTGAPIVASPSNFSNFRTMQFDGVIEFLNQGAAYPRSNQ
                     EVPSSFYTSSINSAFQLGSLDFFERGETLQWKVRPTHVNNPNAGNLSGYAAGGLYPFL
                     ASQYDVHEGVNDPLNPAATQDPISRLPDGILTIGLHGFRIVQPPGPVRIT"
     gene            646..1533
                     /locus_tag="AC16UZ_09795"
     CDS             646..1533
                     /locus_tag="AC16UZ_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247017.1"
                     /translation="MRPDLLLREREVRGLGIGAPQPPANVKTLVPGRSGYLGPSPSEG
                     EASTWGCDAAQRVQLAGGALNTLLVHPEITPFEQLYRRLPSEGMFLPGVSPERPFVFE
                     LGAFRVPDTFDLLLFDLRPDIYRFSGVDAGDTVPVEARRFSSIMGFEVTVDQRHLGNV
                     RFELDPIAIQTTTQAFAPEIRSTEFSQVLQTPPARFNIAAANSFANAAGAGLSLLPQR
                     PYRWGPLSVPFTLYVRSGQTVQVRCVVFRPIPSPIAFIEYDIGGLLIPDEWVSSMTEC
                     MKPMTHASGGRGSAGGGPR"
BASE COUNT          290 a          556 c          545 g          332 t
ORIGIN      
        1 gcccgttcgt tgccgtggct cggtttgcga cgtttcagtc ggcgtttcag ttcaggtccg
       61 tcgacccgca gacgcaggag gaggccacct tccagggtcg ctcattcgga aggtaccggc
      121 cgatccattc cgcctgggac atcaacgacg ggcagcgtgg gtatcagcca acggccggtc
      181 tcgcattccc ggggaccggg gcgcccatcg ttgcgagccc gagcaatttc tcgaacttcc
      241 gcacgatgca gttcgacggg gtcatagaat ttctgaacca gggagccgcc tacccgcgct
      301 ccaaccagga agtgccgtcc agcttctaca cgtcgtcgat caactcggcg ttccagctgg
      361 gtagcctcga cttcttcgag cgcggtgaga cgctgcagtg gaaggtacgg cccacgcacg
      421 tcaacaaccc gaacgccgga aacctctccg gctacgcggc aggcggccta tacccatttc
      481 tcgccagtca gtacgacgtg cacgaaggcg tcaacgaccc gctgaacccg gcggcgacgc
      541 aggacccgat ctcgcgtttg ccggacggca ttctcaccat tggccttcac ggcttccgga
      601 ttgttcagcc acccggacct gtgaggatta cgtaggaggt gccgcgtgag gcccgacctc
      661 ctgcttcgtg agcgcgaagt ccgcggcctg gggatcggag ccccgcagcc tcccgcgaac
      721 gtgaagacgc tcgttcctgg gcgctccggg tatctgggtc cgtcgccctc ggagggcgaa
      781 gcctcgacct ggggttgcga tgctgcgcag cgggtgcagc ttgccggggg cgcgctcaac
      841 acgctgctcg tgcatccgga gatcacgccg ttcgagcagc tgtaccgacg gctgccttcc
      901 gagggaatgt tcctgccggg cgtcagtccg gagcgaccat tcgtgtttga acttggcgca
      961 ttccgagtgc ccgacacatt cgatctcctg cttttcgatc tgcgtccgga catctacaga
     1021 ttcagcggag tcgatgcggg cgataccgtg ccagtcgagg ctcgccgctt ttcgtccatc
     1081 atgggtttcg aggtgaccgt cgatcaaagg cacctcggca acgtacggtt cgagttggat
     1141 ccaatcgcga tccagacgac cacacaggca tttgcccccg aaatccgctc aacggagttc
     1201 tcgcaggtac tgcaaacgcc gcctgcccgc ttcaacatcg cggcggcaaa cagctttgca
     1261 aacgcggccg gcgcggggct ttcgttgctg cctcagcgac cgtaccggtg gggcccgctg
     1321 tctgtgccct ttaccctgta tgtgcgctcg gggcagactg tccaggtgcg ctgcgtcgtc
     1381 tttcggccca tcccgtcccc gattgcgttc atcgagtacg acatcggcgg actgctcatc
     1441 ccggacgagt gggtgtccag catgaccgag tgcatgaagc cgatgacgca cgcgagcggt
     1501 ggacgcggct cggcaggcgg ggggccccga tgagtgtcgg aagctcaccc atagaagttg
     1561 gccgaccggt gtcgccaaaa ctccaccggc ccctggcgga gcggcgggta tcggggatcg
     1621 ctctcgtgtc ggacatgcgg gaagcctact cgtgctcggg aaacgtcggt cggtcatctt
     1681 ctgtgggtga gattccatga gcgtgatgga gagatacgtc ccc
//
LOCUS       DBNHOX010000441         5623 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_934720, whole genome shotgun sequence.
ACCESSION   DBNHOX010000441 DBNHOX010000000
VERSION     DBNHOX010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_934720"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1112)
                     /locus_tag="AC16UZ_09800"
     CDS             complement(96..1112)
                     /locus_tag="AC16UZ_09800"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF006046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR6247018.1"
                     /translation="MDFVGSHILSVDQFTREDIEHVFRVADDMQPYADRERVTRVLQG
                     AILSNMFFEPSTRTRISFGSAFNMLGGEVRETTEVAASSLTKGESLYDTARVLSGYSD
                     VIVMRHPEAGSVAEFAKASRVPVINAGDGPNEHPSQALLDLYTIRKEMESRGRAIDDL
                     RIALVGDLRYGRAVHSLCKLLALFEHVHYKLVSPPELSLPADIVASLRAAGHDVEEHH
                     ELETGIDHVDILYVTRTQEERFPDQEEADRYRGLFRVNQAIYTKNCEPNTVIMHPLPR
                     DSRAQAQELDDDLIENPNLAIFRQTDNGVLIRMALFALVLDVAGQVHNHAQPVTWYTA
                     KRFR"
     gene            1301..2821
                     /gene="gspE"
                     /locus_tag="AC16UZ_09805"
     CDS             1301..2821
                     /gene="gspE"
                     /locus_tag="AC16UZ_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HIR6247019.1"
                     /translation="MSDLAHDEPDADIAQVQDAPLPLPFAFARRFGVLITGETGSREQ
                     ALVACKVQPSLTTLAEIKRVVRRELKLKMVGQDEFERLLTATYARDGSAARQMVEDMG
                     DEMDLASLADSVPETEDLLEQEDDAPIIRLINALLAEAIRENASDVHIETFEKDLIVR
                     FRVDGVMREVVKPKRQLAPLLVSRIKVMARLDIAEKRVPQDGRISLRIGGREVDVRVS
                     TMPASNGERVVLRLLDKQAGRLRLENLGMSNEGLGLLRDIVHKPHGIFLVTGPTGSGK
                     TTTLYASLAELSTQTINILTVEDPIEYNLPGIGQTQVNTKADMTFARGLRAILRQDPD
                     VVMVGEIRDIETAEVAVQASLTGHLVMSTLHTNTAVGAITRLIDMGVEPFLLSSSLVG
                     MLAQRLVRVLCPKCKEHRSATASELEFLDQPSAFVYEARGCDACQGTGYRGRTGIYEL
                     VPVDDDMRQLIHDQVSEQELTAFARQRTPGIRDDGKMKVLSGVTSVQEILRVTLEE"
     gene            2829..4049
                     /gene="gspF"
                     /locus_tag="AC16UZ_09810"
     CDS             2829..4049
                     /gene="gspF"
                     /locus_tag="AC16UZ_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HIR6247020.1"
                     /translation="MAAFEYTALDPRGRQRKGVLEADSVRQIRQLLRDQGLVPLGVEA
                     AGERAVKKPGEGFSFKLPRRIGPLDRVLFTRQLATLIAAGLPVEEALQAVAQQSEKQH
                     IRSLVMGIRSRVLEGHSLASSLASYRSSFSEMYRSTVAAGEQSGHLDKVLDNLANYTE
                     RQFEARRDVEMAMLYPAVLTLLAFGIVGALMVYVVPDMVSVLENMGQGLPASTRFLIA
                     TSELARDYWWALIALVGLVVAGIQWALAQPPIRLAWDRQKLFMPLVSRITRSSNAARY
                     ANTLSILTSSGVPLVEAMNIASEVVSNAWLKRRLADSTQRVSEGTSLRAALEGVGHFP
                     PMLLHMVASGESSGELDSMLEKVAIYQQTEVERIVTTLVRLFEPLMLVVMGGLVMFIV
                     MAILLPILNMNQLV"
     gene            4058..4477
                     /gene="gspG"
                     /locus_tag="AC16UZ_09815"
     CDS             4058..4477
                     /gene="gspG"
                     /locus_tag="AC16UZ_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HIR6247021.1"
                     /translation="MKARIAGFTLIEVLVVVVIIGILGAVIVPNLLGRPDQARVTAAQ
                     SDIRSLANALDIYRLDNFQYPSTDQGLEALESKPTGFPEPKNYNPEGYIKKLPADPWG
                     SPYVYERTEEGFALYSYGADGQEGGEGLNADIFFEDL"
     gene            4449..4997
                     /gene="gspH"
                     /locus_tag="AC16UZ_09820"
     CDS             4449..4997
                     /gene="gspH"
                     /locus_tag="AC16UZ_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HIR6247022.1"
                     /translation="MPTSSSRIFSRPAVRQRGFTLLELLVVVSLVGILTGVVVLGFTG
                     ADTQQEIKGLAQTMAYRVELARQQALTRNREWGIYVDEQAYRFAEYRPEEGEWENQSR
                     RPFDVIDVPEHVSLRLETEGVGELPFADGERLPQILVFSSGEITPFTVYLEFGDARDP
                     WVVSSDGLARARASRSSEIGEA"
     gene            4997..5377
                     /gene="gspI"
                     /locus_tag="AC16UZ_09825"
     CDS             4997..5377
                     /gene="gspI"
                     /locus_tag="AC16UZ_09825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HIR6247023.1"
                     /translation="MGQRGFTLVEVLVALVVFAVMGFTITTRVGDVSSQMFGMERRTM
                     AHWVADNHLNRMRLAQLAADEVLATGLDRERVFMGDREWRLEVDVADTTHPWLRRVEI
                     TVFEATESGEVGPLDHVVAFLGRY"
     gene            5377..>5623
                     /locus_tag="AC16UZ_09830"
     CDS             5377..>5623
                     /locus_tag="AC16UZ_09830"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR6247024.1"
                     /translation="MSAWIAPLTARRQEGFTLLEMLVVFSVFAVLGVVASQIVGRVLG
                     NQQILSERGERLAEIQRAMEIIQRDVMQVSSRGVRDQL"
BASE COUNT          992 a         1763 c         1858 g         1010 t
ORIGIN      
        1 gcctggcgcc aggcgacccg ctttctagcg gagtgccgcg gctacaagac cgtgccgaag
       61 gcctggaccg ccagcgtgcc tcgtatcccc aaaggctagc ggaaccgctt ggccgtatac
      121 cacgtcaccg gctgagcgtg gttgtgcacc tggccggcca catccagcac cagcgcaaac
      181 aaggccatcc tgatcagaac cccgttgtcc gtctggcgga aaatggccag attggggttt
      241 tctatcagat cgtcgtccag ctcctgagcc tgggcgcggg agtcccgtgg cagcggatgc
      301 atgatcaccg tgttgggttc gcagttcttc gtgtagatgg cctggttgac ccggaacagc
      361 ccgcggtagc ggtcggcttc ttcctggtcg ggaaaccgtt cttcctgggt gcgcgtcacg
      421 tagaggatgt ccacgtgatc gatgccggtc tccagctcgt ggtgctcctc cacgtcgtgg
      481 ccggccgctc tgagcgacgc cacgatgtcc gccggcaggg aaagttcggg cggggacacc
      541 agcttgtagt ggacgtgctc gaaaagcgcg agcagcttgc agagggagtg cacggcccgt
      601 ccgtagcgca ggtcacccac cagcgcgatc cggagatcgt cgatggcgcg gccgcggctc
      661 tccatttcct tgcggatggt gtagagatcc agcagcgcct ggctggggtg ctcgttggga
      721 ccgtctccgg cgttgatgac gggcacccgg ctggccttgg cgaactccgc caccgatccg
      781 gcctcgggat ggcgcatcac gatgacgtcg ctgtagccgg agaggacgcg ggcggtgtcg
      841 taaagggatt cccccttcgt cagtgacgac gcggctacct cagtggtctc gcgtacctcg
      901 ccgccaagca tgttgaacgc cgagccgaac gagatgcggg tccgggtgct cggctcgaaa
      961 aacatgttgg agagaatggc gccctgcagg acccgggtga cccgttcccg atccgcataa
     1021 ggctgcatgt cgtccgcgac ccggaaaacg tgctcgatgt cctcacgagt gaactggtcc
     1081 accgacagta tatggctgcc aacgaagtcc aacggcgtcc cccggctgaa tttgcgcaag
     1141 tctacaccta ccgcggcgtc acgggaaaac gagccgccgt tcacgctttg tgatgaccgt
     1201 cacggaagca agggcagcgc cacacgggtt ggaatctttg ggtttagcgg tgcgtgcctg
     1261 ggttgctatc attgggctgc cccagtacga gtaccgccag atgagcgact tggcacatga
     1321 cgagccggac gccgacatag ctcaggtgca ggacgcgccg ttgcccctgc ctttcgcctt
     1381 tgcccgccga ttcggcgttc tgatcacggg agaaaccggc tcccgggagc aggccctggt
     1441 agcctgcaag gtccagccga gcctcaccac cttggccgag atcaagcgcg tggtgcggcg
     1501 cgagctgaag ctgaagatgg tgggtcagga cgagttcgaa agactgctca cggcgaccta
     1561 cgcccgggac ggctccgccg cccgccagat ggtcgaggac atgggcgacg agatggatct
     1621 cgcaagcctt gccgactcgg tcccggagac cgaagacctt ctcgagcagg aggacgatgc
     1681 gcccatcatc cggctcatca acgcgctgct ggccgaggcc attcgcgaaa acgcttccga
     1741 cgttcacatc gagacgttcg agaaggacct catcgtccgg ttccgggtcg acggggtgat
     1801 gcgggaagtg gtcaaaccca agcgccagct ggcgcctctg ctggtctccc ggatcaaggt
     1861 catggcccgc ctggacatcg ccgagaagcg ggttcctcag gacggccgaa tctccctgcg
     1921 cataggcggc cgggaagtcg acgtgcgggt ttccaccatg ccggccagca acggtgaacg
     1981 ggtggtgctt cggcttctgg acaagcaggc aggacggttg aggctggaaa atctgggcat
     2041 gtcgaacgaa gggctcgggt tgctgcgcga catcgttcac aagcctcacg gcatcttcct
     2101 ggtcaccggc cccaccgggt cgggtaagac caccacgctc tacgcctccc tggccgagct
     2161 gagcacccag accatcaaca tcctgacggt cgaggacccc atcgagtaca acctgcccgg
     2221 catcggccag acccaggtga ataccaaggc ggacatgacc ttcgcccgcg gccttcgcgc
     2281 cattctgcgt caggaccccg acgtggtgat ggttggcgag atccgggaca tcgagactgc
     2341 cgaggtcgcc gtccaggcaa gcctcacggg tcacctcgtg atgtccaccc tccacaccaa
     2401 cacggccgtg ggagccatta cccgcctgat cgacatgggc gtcgagccct tcctgctgtc
     2461 ctcgagcctg gtggggatgc tggcgcagcg gctcgtccgc gtgctctgtc cgaagtgcaa
     2521 ggaacaccgc agcgccacgg cctcggagct agagttcctc gaccagcctt ccgcgttcgt
     2581 ttacgaggcg cgcggctgcg atgcgtgcca gggcaccggc tatcgcggcc gcacgggcat
     2641 ctacgagctg gtccccgtgg acgacgacat gcgccagctc attcacgatc aggtctccga
     2701 gcaggagctc acggcctttg cgcgccagag aacgccaggc atccgtgacg acggcaagat
     2761 gaaggtgctc tccggcgtga cctcggtgca ggaaatcctg agggtcaccc tggaggaata
     2821 gccccgacat ggcggcattc gagtacacag cgcttgatcc cagagggcgc caacgcaagg
     2881 gcgtcctgga ggcggacagc gtccgccaga tccgtcagct gctgcgcgac cagggtctgg
     2941 ttcccctcgg cgtggaagcc gccggggaac gcgccgtcaa gaaaccggga gagggtttct
     3001 cctttaagct gccgcgtcgg atcggaccgc tggaccgcgt gctgttcacc cgtcagctcg
     3061 ccaccctcat cgcggccggg ctgccggtcg aagaggctct gcaggcggtg gcgcagcaat
     3121 ccgaaaagca gcacatacgc tccctggtca tgggtattcg ctcgagagtg ctggaaggcc
     3181 actcgctggc ctcgagcctc gccagctacc gtagcagctt ttccgagatg taccgctcca
     3241 cggttgccgc cggcgagcag tcggggcatc tcgacaaggt gctcgataac ctcgccaact
     3301 acacggagcg gcagttcgag gcccgccggg acgtggaaat ggccatgctc tatccggcgg
     3361 tgctgaccct tctggccttc ggcatcgtcg gcgcgctgat ggtctacgtg gtgccagaca
     3421 tggtcagcgt gctcgagaac atggggcagg ggctgccggc cagcacccgt ttcctcatcg
     3481 ctacctcaga actggcacgg gattactggt gggcgctcat cgccttggtg ggactggtgg
     3541 ttgctgggat tcaatgggcg ctcgcccagc cgcccatccg tctggcctgg gaccggcaga
     3601 agctcttcat gcccctcgtg agccgcatca cgaggagcag caacgcggcc cgttacgcaa
     3661 ataccttgag catcctgacc agcagcgggg tgccgctcgt agaggccatg aacatcgcca
     3721 gcgaggtggt ttccaatgcg tggctcaagc ggcggcttgc cgactcaacc cagcgtgtga
     3781 gcgaaggaac gagcctcagg gcggcgctgg agggtgtggg gcatttcccg cccatgctgc
     3841 tgcacatggt ggccagcggc gagtccagcg gcgagctcga cagcatgctc gaaaaggtgg
     3901 ccatttatca gcagactgag gttgagcgga tcgtcacaac ccttgtgaga cttttcgaac
     3961 cgctgatgct ggttgtgatg gggggccttg tcatgttcat cgtgatggct attctgctgc
     4021 ccatactcaa catgaaccag ctggtgtgac ggtgaagatg aaagcaagga ttgcgggttt
     4081 cacgctgatc gaggtgctgg tggtggtggt catcatcggc atcctgggcg ccgtcatcgt
     4141 cccgaacctg cttggccggc ccgaccaggc gcgcgtcacg gcggcccaga gcgacatccg
     4201 gtccctggcc aacgcgcttg acatctatcg gctggacaac ttccagtacc cgtccacaga
     4261 ccagggcctg gaagccctgg agtccaagcc cacggggttc ccggagccca agaactacaa
     4321 ccccgagggc tacatcaaga agcttcccgc ggatccctgg ggctctccct acgtctacga
     4381 gcgcaccgag gagggctttg cgctttactc gtacggcgca gacggccagg aaggcggaga
     4441 gggactcaat gccgacatct tcttcgagga tctttagccg gccggcggtc cgtcagcgcg
     4501 gcttcaccct gctggagctg ctcgtggtgg tgagcctggt cgggatcctc accggtgtgg
     4561 tcgtactggg tttcacgggc gcagacactc agcaggagat caaagggctc gcccagacaa
     4621 tggcttaccg cgtggagctc gcccgtcagc aggcgctgac ccgcaatcgc gaatggggca
     4681 tctacgtgga cgagcaggcg tatcggttcg ccgagtatcg ccccgaagag ggcgagtggg
     4741 aaaatcagtc ccggcgcccg ttcgacgtca tcgacgttcc cgagcacgtg agcctgcggc
     4801 tggaaaccga gggggtaggg gagttgccct tcgcggacgg agagaggctg ccgcagatcc
     4861 tggtgttctc ctccggcgag atcacgccgt tcaccgtcta tctcgagttc ggcgacgctc
     4921 gtgacccctg ggtcgtcagc tcagacgggc tcgcgcgtgc ccgcgcctcc aggtccagcg
     4981 agatcggaga ggcttgatgg ggcagcgcgg attcaccctg gtcgaggtcc tcgtggcgct
     5041 ggtggtcttc gccgtgatgg gattcaccat caccactcgc gtcggcgacg tctccagcca
     5101 gatgttcggc atggagcgac gcaccatggc ccattgggtg gcggacaatc atctgaaccg
     5161 catgcgtctg gcccagctgg ccgccgacga ggttctggcc accggccttg atcgcgaacg
     5221 ggtattcatg ggggatcggg agtggcgtct tgaagtcgac gtggccgaca ccacgcatcc
     5281 ctggctgcgt cgcgtggaga tcacggtgtt cgaggcgacg gagtcggggg aggtcggacc
     5341 tctggaccac gtcgtggcct tcctggggcg ctactgatgt cggcttggat agctccactc
     5401 acggcacggc ggcaggaggg cttcaccctc ctggaaatgc tggtggtatt cagtgttttc
     5461 gccgtgctgg gcgtggtggc cagccagatc gtcggtcggg tgctcggcaa ccagcagatt
     5521 ctgagcgagc gtggcgagcg cctggccgag atccagcgcg ccatggaaat cattcagcgc
     5581 gacgtgatgc aggtcagcag ccgcggcgtg cgcgatcagc tgg
//
LOCUS       DBNHOX010000442         2513 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1384431, whole genome shotgun sequence.
ACCESSION   DBNHOX010000442 DBNHOX010000000
VERSION     DBNHOX010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1384431"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="AC16UZ_09835"
     CDS             complement(<1..166)
                     /locus_tag="AC16UZ_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6247025.1"
                     /translation="MILTLITISRRGPDVAQQEEGKMQTNEKFEIRGINHIALVCRDM
                     KETVDFYSGVL"
     gene            230..886
                     /locus_tag="AC16UZ_09840"
     CDS             230..886
                     /locus_tag="AC16UZ_09840"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6247026.1"
                     /translation="MPRLKQVSRAEATENVRKYYDALFGERDPVAEPGTATGTPGNWW
                     TVFALVPYVFDHATSHFGMFGMFADKSISQLDGKVRELGIMRAGYTQGSQFVFSQHCK
                     AARRFGISDEQIEAIPHWEVSDAFDAKERAVLAWADALILQGGRASDALFEALHSHLS
                     DEDILELTYHINGYNLHAVCCKALRLEYDDVPERIREVPVPAEGETADWAGGAWRREE
                     "
     gene            891..1478
                     /locus_tag="AC16UZ_09845"
     CDS             891..1478
                     /locus_tag="AC16UZ_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6247027.1"
                     /translation="MSDSHRIHYGGLHHLALVCRDMERTVDFYTNVLDMKLKKGFDLD
                     GGYGQHFFFDMGGGNELAFFWFRDAKDAQPGVASAAHLVGSGEGSITSAHGSMNHVAF
                     DVAPDQIEAYREKLVSRGVNCTPLVNHDDVVTGEESRRGSGISAKTWLRSFYFYDPDG
                     IMLEFCATLNTGSPEVELPVNAAGIKANGEPITGA"
     gene            1475..1951
                     /locus_tag="AC16UZ_09850"
     CDS             1475..1951
                     /locus_tag="AC16UZ_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6247028.1"
                     /translation="MTNRDQTSTASPLEHASGPALLRELVHATYWLDDGLQAYMKEHA
                     GLSLPRAQSMMMVYIAEGVDSPSDLARRLRVSKQAIRQGLKELMAKDMVTLEPDPGNR
                     RQKVVRFTKHGRALRQVAWEGLQNLERELGRRLGGDRLAALHDALFLDWGDVPGEP"
     gene            complement(2044..2358)
                     /locus_tag="AC16UZ_09855"
     CDS             complement(2044..2358)
                     /locus_tag="AC16UZ_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247029.1"
                     /translation="MSAVNIIRIVALVLAVVGAFVTIPYAAAALVILGLVAGFMGVDE
                     ERRLMVLLMAVALAVVADGLGPIPAVGDYLTAILTNLSGVFDGLAIAVILTAIYERIT
                     QG"
BASE COUNT          513 a          794 c          764 g          442 t
ORIGIN      
        1 ccagaacgcc cgaatagaag tccaccgttt ccttcatgtc ccggcagacc agggcgatgt
       61 ggttgattcc ccgaatctcg aatttttcgt tcgtctgcat ctttccctcc tcctgttggg
      121 ctacgtccgg cccgcgccgg ctgatcgtaa tcagcgtcaa tatcattgac tatccgggga
      181 gttgtcaata gcatttttcc agcaaaaact gaatacaggg gaggatgaaa tgccaagact
      241 gaaacaggtt tcccgggccg aagcgaccga gaacgtgcgc aaatattacg acgcgctttt
      301 cggggagcgc gacccggtcg cggaacccgg tacggcaacg ggcacgccgg gcaactggtg
      361 gacggtcttc gccctggtac cctacgtgtt cgatcatgcc accagccatt tcggcatgtt
      421 cgggatgttc gccgacaaaa gcatcagcca gctcgacggc aaggtcaggg agctcggcat
      481 catgcgcgcc gggtacaccc agggcagcca gttcgtattt tctcagcact gcaaggcggc
      541 gcggcgcttc gggatcagcg acgagcagat cgaagccatt ccccattggg aggtctcgga
      601 tgccttcgac gccaaggagc gcgccgtgct ggcgtgggcc gacgcgctga ttcttcaggg
      661 cgggcgggcg tcggatgcgc tgttcgaggc cttgcactcc cacctctcgg acgaggacat
      721 cctcgagctc acctatcaca tcaacggcta caacctgcac gccgtctgct gcaaggctct
      781 gcggctggag tacgacgacg ttccggagcg cattcgcgag gtgccggttc ccgccgaggg
      841 agagacggcc gactgggccg gcggggcctg gcggcgcgaa gagtaagccc atgagcgaca
      901 gccacagaat tcactacggc ggtctgcatc atctggcact ggtctgccga gacatggagc
      961 ggacggtgga cttctacacc aacgtgctgg acatgaagct aaagaagggc ttcgacctgg
     1021 acggcggcta cggccagcac ttcttcttcg acatgggcgg cggcaacgag ctggcgttct
     1081 tctggttccg cgatgcaaag gacgctcaac ccggggtcgc cagcgccgca catctggtcg
     1141 gaagcggaga gggctcgatc acctcggctc acggctccat gaaccacgtc gctttcgacg
     1201 tggctccgga tcagatcgag gcttatcggg agaagctggt ttcgcgcggc gtgaactgca
     1261 cgcccctggt caaccacgac gacgtcgtca ccggagagga atctcgacgc gggtcgggca
     1321 tcagcgcgaa gacctggctt cgctcgttct acttctacga cccggacggc atcatgctgg
     1381 agttctgcgc gaccctgaac accggttccc cggaagtgga gctgccggta aacgctgcag
     1441 ggatcaaggc caacggcgag ccgatcaccg gcgcgtgacc aaccgcgatc agacatccac
     1501 ggcatcaccg ctcgagcacg cctcgggacc cgccctcctg cgcgagctgg tgcacgccac
     1561 ctactggctg gacgacgggc tgcaggccta catgaaggag cacgcgggcc tatcgctccc
     1621 gcgcgcccag tccatgatga tggtctacat tgccgagggc gtggatagcc cgtcagacct
     1681 cgcccgacga cttcgcgtga gcaagcaggc catccgccag ggcctcaagg agctgatggc
     1741 taaggacatg gtcacgctgg aacccgatcc cggcaaccgc agacagaagg tggtccgctt
     1801 caccaagcat ggccgcgcgc tgcgacaggt ggcctgggaa ggacttcaga atctggaacg
     1861 ggaactgggg cgacgactcg gcggggaccg cctggccgcc ctgcatgatg cccttttcct
     1921 ggattggggc gacgtccccg gagagccctg atcagggctc atccgggagc cgtctcattt
     1981 agtgaaggtt gccgcgggca atccaacggt catcgccaga cacccgcgaa accagttgcc
     2041 gcgttacccc tgggtgatgc gctcgtaaat ggccgtcagg atgaccgcaa tggccaggcc
     2101 atcgaacaca ccgctgaggt tggtcagtat ggcggtcagg taatcaccga cagccggaat
     2161 tggccccaaa ccgtcggcca ccactgccaa cgccacggcc atcaacagca ccatgaggcg
     2221 acgttcctcg tccacaccca tgaaccccgc taccaggccg agaatgacca gggcggcggc
     2281 ggcataggga atggtaacaa acgcgccgac aactgccaga accagcgcaa cgatccgaat
     2341 aatgtttaca gcactcatac tcattagcga gctcccctca ctgtgaattc acgtttactg
     2401 cattgttttc ctcgcgctcg gactgcaggc gcaactcgaa tttagcacaa cacgtcacac
     2461 ggccgcgaac agatcgttga actgctcgtt attcgacggc ggcgatgctt cgg
//
LOCUS       DBNHOX010000443         2447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_869504, whole genome shotgun sequence.
ACCESSION   DBNHOX010000443 DBNHOX010000000
VERSION     DBNHOX010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_869504"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1687)
                     /locus_tag="AC16UZ_09860"
     CDS             complement(<1..1687)
                     /locus_tag="AC16UZ_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6247030.1"
                     /translation="MTFLTLVEKAAEALRASQRISLRALRREFSLNDELLADLVDELV
                     NVRGVASEEGGVLVLQDAEMAPAKPAMPDRVPAEYTPKHLADRILKSRAALTGERKQV
                     TVLFADVVGSLDIAGRLDPEEWHRVLDRFFKVLTEGVHRFEGTVNQYTGDGIMALFGA
                     PLALEDHAQRACYAALALREPLRDLNDQVRREHGLNFVVRMGINSGEVIVGAIGDDLR
                     MDYTAQGHTVGLAQRMEQLAPADSACLSEHTARFVADQFDLRDLGSFELKGVGGETGV
                     YELLGVDAQRTRAQTPFVGRRSELATLSAAMDRALAGGGQVFGVVAEAGTGKSRLCGQ
                     FVEQCRQDGLTVYEAHCPSHGVSVPLLPILELLRDYFGIHTEDSAQAAREKIAGRLIL
                     LDAEFGEMLPLLFDFLGVTDPEDPLPEMHTEVKQRRMYEFMRRLVQLHAAREPLVVFV
                     DDLHWIDPASDRFMAELVSAVADTRTFLLINFRPEYSAEWTHRSYYQQLPLVPLGLDE
                     VSEMVGNLLGTDASLEGVAEKIFERTRGNPFFTEEVVQDLVESSTLASTPDGYR"
     gene            1808..2425
                     /locus_tag="AC16UZ_09865"
     CDS             1808..2425
                     /locus_tag="AC16UZ_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247031.1"
                     /translation="MDLVTTTQVLGNVGEFIGAIAVVATLFYLALQVREGKKATEANT
                     ESLDQSRQVAMAQVLQNRVRTNVEISRWVAESDHLLRIESEVRSAAATDGFISALNGL
                     EPIDRTRYTLYLRLAPWHMDSLFQLHAAGLLDDETYDCSFSGVEGVLETWTALDDAGM
                     AQTLRPSFRREIERRLAASRSGEKPSGIMGFNPFSTHRPEPTQPG"
BASE COUNT          487 a          806 c          728 g          426 t
ORIGIN      
        1 gccggtatcc gtcgggagtc gatgcgaggg tcgaagactc taccaggtcc tgaaccacct
       61 cttccgtgaa gaaggggttg ccccgcgtgc gctcgaagat cttctcggct accccctcga
      121 gggacgcatc cgtgccgagc agattcccca ccatctctga aacttcatcc agaccaagcg
      181 ggacgagcgg cagctgctgg tagtaggaac gatgcgtcca ttccgcagag tactcgggcc
      241 ggaagttgat cagtagaaac gtccgcgtat cagcaaccgc ggataccagc tccgccatga
      301 agcgatcgct ggcaggatcg atccaatgca gatcatctac gaagacgacg aggggctcac
      361 gagccgcgtg cagctgaacg aggcgccgca tgaactcgta catccgccgc tgcttcacct
      421 ccgtgtgcat ctccgggaga ggatcctcag ggtcggtaac gccgaggaag tcaaacagga
      481 gaggaagcat ctcgccaaac tcggcatcca gcaggatcag acgaccggca atcttctccc
      541 gcgcggcctg ggccgagtcc tcggtgtgaa taccgaagta gtcccgcagg agctccagga
      601 tgggaagcag gggaaccgag acaccgtgcg atgggcagtg cgcttcgtaa accgtcagcc
      661 cgtcctgccg gcactgctca acgaactggc cgcagagccg gcttttgcca gtgccggctt
      721 cggctaccac cccgaacacc tgcccgccgc ccgcgagcgc gcgatccatg gcagccgaaa
      781 gcgtcgccag ctcgctgcgg cgtccgacaa agggcgtttg cgctctcgtc cgctgggcat
      841 cgacgccaag cagctcatag acccccgtct ctccgccgac acccttcagc tcgaaactgc
      901 ccagatcccg cagatcgaac tggtccgcca cgaagcgtgc ggtgtgctca ctcagacacg
      961 cgctgtcggc cggcgccaac tgctccatgc gctgggcgag gccaacggtg tgaccctggg
     1021 ccgtgtaatc catgcgcaga tcgtcgccga tggccccgac gatgacctcg cccgagttga
     1081 tccccattct caccacgaag ttcaagccgt gctcgcgacg gacctggtcg ttgagatccc
     1141 gcaacggttc acgcagcgca agagccgcgt agcaggcgcg ctgagcatga tcttccaggg
     1201 cgaggggcgc tccgaacagc gccatgatcc cgtcgcccgt atattggttc accgtgcctt
     1261 cgaagcgatg aacgccttcc gtcaaaacct tgaagaaccg atcgagaaca cggtgccact
     1321 cctcgggatc cagccggccg gcaatgtcca gcgagcccac cacgtcggca aagagcaccg
     1381 tcacctgctt gcgctcgccc gtgagcgcgg cccgggattt caggatgcga tcggccagat
     1441 gcttgggggt gtactccgcg gggacccgat ccggcatggc aggctttgcc ggcgccatct
     1501 cggcgtcctg aagcaccaga acgcccccct cttcgctcgc gactccgcga acgttgacga
     1561 gctcgtccac caggtcggcg agtagctcgt cgtttaggct gaactcccgg cgcagcgccc
     1621 gcagcgaaat tctctgcgaa gcccgcaagg cctcagcggc tttctcgacc agtgtcagga
     1681 aggtcatgcg ccctctcctc ctacgctatg aaaacacagg tttccacgaa cgcaccagcc
     1741 gttcgccgcc actacgcccg cccggcgatc tacgctagcc tgtcgccgtt cacgggagga
     1801 gataatcatg gatctagtca ccaccactca ggttctgggc aatgtcggcg aattcatagg
     1861 cgcaattgct gtggtcgcga cgttgttcta tctggcgctg caagtgcgcg agggaaagaa
     1921 ggccaccgag gcaaacaccg agtcactgga ccagagccgg caggttgcga tggctcaggt
     1981 gctgcagaac cgggttcgca ccaacgtgga gatctccagg tgggtagcgg aaagcgatca
     2041 cctgctgcgc atcgagtcgg aagtccgaag cgcagcggcg actgacggtt tcatcagcgc
     2101 tttgaatggc ctcgagccca ttgatcgcac ccgatacacc ctctatctca ggctagctcc
     2161 gtggcatatg gactcgctgt ttcaacttca cgcagccggg ctgctggatg acgagaccta
     2221 cgattgctca ttcagcggcg ttgagggggt tcttgaaacc tggacggcat tggatgacgc
     2281 cggcatggcg cagacgcttc gccccagctt tcgccgcgag atcgagcgga ggctcgcagc
     2341 ctcgcgctca ggcgagaagc cttccgggat catgggtttc aacccattct ccacgcatcg
     2401 gccggaaccc actcaaccgg gttgagacgc gacgactaat ctgttgc
//
LOCUS       DBNHOX010000444         8109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1705995, whole genome shotgun sequence.
ACCESSION   DBNHOX010000444 DBNHOX010000000
VERSION     DBNHOX010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8109
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1705995"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="AC16UZ_09870"
     CDS             complement(<1..1068)
                     /locus_tag="AC16UZ_09870"
                     /inference="COORDINATES: protein motif:HMM:NF025114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6247032.1"
                     /translation="MPEGPERFDAVVVGAGFAGLYMLLRLRELGMRVVVFEAGDGVGG
                     TWYWNRYPGARCDAESMGYSYSFSPELEQEWEWTERYATQPEILRYADHVAERFDLKR
                     DIRFETRVVAARYREAGNVWAVATDRGDEVEARFCIMATGCLSVPQRPDIPGLDDFQG
                     PTYVTGEWPHEGVDFTGLRVGLVGTGSSAIQAVPVIAAQAEHLTVFQRTANFSVPAKN
                     RPLDPDWVREFKERYRDYREDSRHGLVSDFGALVLPDAERKRAGGSFRSVPAAERDAR
                     YEKLWEVGGAYFMAAFEDTLLDPEANESAAEFVREKIREIVEDPETAERLCPTSHPIG
                     TKRICVDTGYYETYNRPNVTLV"
     gene            complement(1061..1513)
                     /locus_tag="AC16UZ_09875"
     CDS             complement(1061..1513)
                     /locus_tag="AC16UZ_09875"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6247033.1"
                     /translation="MTLDELLEIEAIKQMRYLYSHYYDGNMLDELISLFTEDAVCEYG
                     EDFGGDWVGKAQIRERYHDFLYGSGRAVHSQMHAVTNPWIRLKSDTQALGRWYQLNLN
                     VAEGAENPLTLFGIYDDVYRKVDGEWKIHRTRIDFLWPRRHFYGIRDA"
     gene            complement(1530..2498)
                     /locus_tag="AC16UZ_09880"
     CDS             complement(1530..2498)
                     /locus_tag="AC16UZ_09880"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017904614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6247034.1"
                     /translation="MTVKTTTAAVAEAIDQEMARDDRVFVAGEDVRVGGPFGTDAGLH
                     AKYGDRRVVNTAISELALAGLGVGAAAVGLRPIIEIMIMDFMCVAMDQIVNQMAKMKY
                     MLGGKPTLPIVVTTHAGIDSSSAAQHSGSLEAWFCHVPGLKVVMPSNAYDAKGLLISA
                     IRDDSPVIFIHNKRCLQTSADVPDEAYTVPLGVADVKREGSDVTIVAIGRMVDEALEA
                     ADELQGDGVSAEVIDPRTLSPLDMETILDSVRKTTRAVVAHEAVRFCGIGAEIAATIS
                     DEAFDYLDAPVKRVAAPFTPIPFAPVLENLYLPTRESILAAVREIV"
     gene            complement(2516..3478)
                     /locus_tag="AC16UZ_09885"
     CDS             complement(2516..3478)
                     /locus_tag="AC16UZ_09885"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR6247035.1"
                     /translation="MDLPEEQMIRMLETMVRIREFDERAALLMEQARIHGSVHLYCGE
                     EAVGVGVCEALNPDDYITSTHRGHGHLIAKGGDPRLMYAELFAKSTGYNRGKGGSMHI
                     AALELGMIGANGIVGAGAPIALGVAFASAYQDNGRVTACFFGDGATNEGAFHEALNMA
                     AILKAPVVFVCENNLYGEWTPASQQTSVRDIADRSASYGIPGVIVDGMDIMAVHEAVV
                     EAVARARRGEGPTLLECKTYRFYDHVGRDFGILKRDPDEVAAWRARDPITLWRAELIA
                     QGVLDEAGADAITERAQAEMEDAIAYAEGSPDPEPEDLMRDIYS"
     gene            complement(3560..4447)
                     /locus_tag="AC16UZ_09890"
     CDS             complement(3560..4447)
                     /locus_tag="AC16UZ_09890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6247036.1"
                     /translation="MRLGFSIPNNQGVERVSDLVRLAVEAEELGYASVWVSEHLWHAG
                     YVARRLGEKPYHEPLTVLTAIAAATRQVRLGTSVLVLPWHHPVRLAKTIATLDDLSDG
                     RVSLGVGVAVTEDEFENLGVDFATRGRRADDALAAIKALWTQEFPRHEGPFYQFSGMR
                     FEPKPRQSPHPPILVGGGAPPALRRAARFADGWHALGQSPESLAESMKMLEESCRAVG
                     RDVADLDISIRCVIEIIGEPWDRPVTRRRTLKGTEREIAETIAAYAEAGVDEIVLDAN
                     LTDLEATRAVMHRGIRFQD"
     gene            4583..5839
                     /locus_tag="AC16UZ_09895"
     CDS             4583..5839
                     /locus_tag="AC16UZ_09895"
                     /inference="COORDINATES: protein motif:HMM:NF047238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR6247037.1"
                     /translation="MQADIDATAGRHLRGRYAVVGVGETGYVRGSGCTTRALGTRAVR
                     AALADAGLEPGDVDGMLSYSYNDSTFSPFIAGDLGIRLNFYMDVYGGGSSIEALIGIA
                     MGVIEAGMCRTVVIFRAMNGYSQVRIGGTGGRGAVAPLGGDALFNRAFGQMSAGQMFC
                     QSFMRHMYDYGTTPEQVAMVKVVHSEHASNNPKAYYRQRVSVDDVVNSRMIVKPLHLL
                     DCCVETDNGTAVVVTGIDRARDCRHTPAVIQSVVGRCSKRRSDMHYQSGPISTVAGHY
                     AKDILWPNAGVGPEDIDVTGSYDAFTFTTMLQLEDYGFCKKGEGGDYVSSGITRLGGA
                     RPNNTSGGHLCEGYTHGINMVIENVRQLRHDVDDFCPIGSDGRRVHTYDYTEGGCRQV
                     KDCELTANLGWATPGTGSAMVMARGG"
     gene            5842..6363
                     /locus_tag="AC16UZ_09900"
     CDS             5842..6363
                     /locus_tag="AC16UZ_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6247038.1"
                     /translation="MALEGEYLGMPVLVDDLDQENLAFFGHCGRGELCLQQCKACGLK
                     RYPPTTACPWCASPDATWEPVAGKGTVYSYGEIHHAIQPAFRQNSPYLLLLVELDEQR
                     DLPAPHDGLRMTGNLATEDGDLAPAELVRSVGIGSRVRVVFKQLGDGIALPLWCLDES
                     AEQPQTPWRYPQE"
     gene            6421..6834
                     /locus_tag="AC16UZ_09905"
     CDS             6421..6834
                     /locus_tag="AC16UZ_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIR6247039.1"
                     /translation="MLDPTTITIFGLVLIKLLLVASIGMHRLYIASTSDKAPNTFLPT
                     GEDVGRFAVRLARAHANCYEFLPFALAVLVYAAATESSALTDGLAYYFLIARIGQSAT
                     HLVSTSTTAVFIRFAFFLAQVVILLVWCLGFLGAL"
     gene            6923..7927
                     /locus_tag="AC16UZ_09910"
     CDS             6923..7927
                     /locus_tag="AC16UZ_09910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6247040.1"
                     /translation="MPELRVGVCYDFRNPPDSGVSDARLYAEVMEQVRWLDGIGADLV
                     WFTEHHFVDDGYLPSWTPVAGAMAAVTENVRFGTDICLMPFNHPVRLAEDLAVLDNLS
                     GGRVELGIGMGYAPHEFRGFGLPVSRRVSLMNEGIEVLQRCFTGERFSYHGKRYQLDD
                     VRITPGYVQPGGPPLWIAAMSEGGAARAARYEANFLPQGNRARVYDTWVDALESSGRS
                     PADHRIGIIRSILVTDDRETDWPVIRAAERYRMALYQRFFEESGEGFGDPREAIPQTW
                     IVGNVDQCVAELQQFISDFRITDIVTMAVPPGLRAEQMAPSLERLFTEVVPRLKGGRP
                     "
     gene            7924..>8109
                     /locus_tag="AC16UZ_09915"
     CDS             7924..>8109
                     /locus_tag="AC16UZ_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6247041.1"
                     /translation="MRVVVIGAGIGGSALALALDRLGIEHVVLEQAPEFTDVGAGIQL
                     SPNGVRILESLGLRDALK"
BASE COUNT         1341 a         2770 c         2626 g         1372 t
ORIGIN      
        1 caccagcgtc acgttggggc ggttgtaggt ctcgtaatag ccggtgtcca cgcagatccg
       61 tttggttccg atgggatgcg acgtggggca caggcgctct gcggtttccg gatcctcgac
      121 gatctcacgg atcttctcac gcacgaactc cgccgcgctc tcgttggcct cgggatccag
      181 cagggtgtct tcgaaggccg ccatgaaata cgcgccgcca acctcccaga gcttttcgta
      241 gcgggcatcg cgctcggccg ccggcacgga acgaaacgat ccgccggccc gcttgcgctc
      301 ggcgtcgggc agcacgaggg cgccgaagtc ggacaccagc ccgtgtcgcg aatcctcacg
      361 atagtcccgg tagcgctcct tgaactcgcg cacccagtcg ggatccagcg gacggttctt
      421 ggcgggaacg ctgaaattcg ccgtgcgctg aaataccgtc aggtgctcgg cctgggcggc
      481 gatgacgggc acggcctgga tggccgatga gcccgtgccc acaaggccga cccgcaggcc
      541 ggtgaaatcc accccctcgt gaggccattc tccggtcacg tacgtcgggc cctgaaagtc
      601 atccaggccc gggatgtcgg gacgctgggg aacggacagg cacccggtcg ccatgatgca
      661 gaagcgggct tccacctcgt ctccgcggtc cgtggccacg gcccagacgt tgccggcttc
      721 ccgatagcga gccgccacca cccgcgtctc gaagcgaatg tcccgcttga ggtcgaagcg
      781 ttcggcgaca tggtcggcgt agcgaagaat ctcgggctgg gtggcgtagc gctcggtcca
      841 ctcccactcc tgctcgagct cgggcgagaa cgagtacgag tagcccatgc tctccgcgtc
      901 gcagcgggcg cccggatacc ggttccagta ccaggtgccg ccgacgccgt cacccgcctc
      961 gaaaaccacc acccgcatgc ccagctcacg gagccgcagc agcatgtaaa gcccggcaaa
     1021 gccggcgccg acgacgacgg cgtcgaagcg ttccgggcct tcaggcatcc cggatcccgt
     1081 agaagtggcg ccggggccag aggaagtcga tgcgggtccg gtggatcttc cactccccgt
     1141 ccaccttgcg atacacgtcg tcgtagatgc cgaagagggt cagcgggttc tccgcgcctt
     1201 cggcgacgtt caggttcagc tggtaccagc ggccgagcgc ttgggtatcg ctcttcaaac
     1261 ggatccacgg attggtcacc gcgtgcatct gggaatgtac cgcgcgtccg ctgccataga
     1321 ggaagtcgtg gtaacgctcg cgaatctgcg ccttgcccac ccagtcgccg ccgaaatcct
     1381 cgccgtactc gcatacggcg tcctcggtga agaggctgat cagctcatcg agcatgttgc
     1441 cgtcgtagta gtgcgagtac aggtaacgca tctgcttgat ggcttcgatc tcgagcaact
     1501 cgtcaagggt cacggccggc tcctcctgtt cagactattt cccgaacggc cgccaggatg
     1561 gattcccggg tcggcagata gaggttttcc aggaccggcg cgaagggaat cggcgtgaac
     1621 ggcgcggcca cccgcttaac cggcgcgtcg aggtagtcga acgcctcgtc ggagatggtc
     1681 gctgcgatct cggcaccgat gccgcagaac ctgaccgcct cgtgggccac caccgcccgc
     1741 gtcgtcttgc gaacggagtc gagaatcgtc tccatgtcca gcggcgacag ggtccgcggg
     1801 tcgatcacct cggcgctgac accatcgccc tgcagctcgt cggccgcttc cagagcctcg
     1861 tcgaccatgc ggccgatggc cacgatggtg acgtcgctgc cctcccgctt gacgtcggcc
     1921 acgccgaggg gaaccgtgta ggcctcgtcg ggcacgtcgg ccgaggtctg caggcagcgc
     1981 ttgttgtgaa tgaagatcac cgggctgtcg tcccggatgg cggagatcag caggcccttg
     2041 gcgtcatagg cgttggaagg catgaccacc ttcaggccgg gcacgtggca gaaccacgcc
     2101 tcgaggctgc ccgagtgctg ggccgcgctg ctgctgtcga tacccgcgtg ggtcgtcacc
     2161 acgatgggca gcgtgggctt tccgccgagc atgtacttca tcttggccat ctggttcacg
     2221 atctggtcca tggccacgca catgaagtcc atgatcatga tctcgatgat gggtcgcaga
     2281 cccaccgccg cggccccgac cccgaggccc gcgagggcca gctccgatat cgccgtgttc
     2341 accacgcgcc gatcgccgta cttggcgtga agaccagcgt ccgtgccgaa cggcccgccc
     2401 acgcggacgt cctctccggc gacgaacacc ctgtcgtccc gcgccatttc ctggtcgatg
     2461 gcctctgcca ccgcggccgt cgtcgtcttt actgtcaccc tgcccccttc gatattcagc
     2521 tgtagatgtc gcgcatcagg tcctcgggct cgggatccgg gctgccctcc gcatacgcga
     2581 tggcgtcctc catctccgcc tgggcgcgct ccgtgatggc gtcggcgccg gcttcgtcga
     2641 gcaccccctg agcgatcagc tcggcccgcc agagcgtgat ggggtcccgg gcgcgccatg
     2701 cggccacctc gtcgggatcc cgcttgagaa tgccgaagtc gcgcccgacg tggtcgtaga
     2761 agcggtacgt cttgcattcc aggagcgttg gcccctcgcc cctgcgggct cttgccacgg
     2821 cttccaccac cgcctcgtga acggccatga tgtccatgcc gtcgacgatg acccccggaa
     2881 tgccgtatga ggccgaacga tcggcgatgt cccgcaccga cgtctgctgg gaagccggcg
     2941 tccactcgcc gtagaggttg ttctcgcaaa cgaagaccac cggcgccttg agaatggcag
     3001 ccatgttgag cgcttcgtga aaggcaccct cgttggtcgc gccgtcgccg aagaaacagg
     3061 ccgtgacccg gccgttgtcc tgataagcgc tggcgaaggc gacgcctagc gcgatgggcg
     3121 cccccgcgcc gacgatgccg ttggcaccga tcatgcccag ttccagcgcg gctatgtgca
     3181 tggagccgcc cttaccccgg ttgtagccgg tggacttggc gaacaactct gcatacatca
     3241 gccggggatc gccgcctttg gcgatgaggt gcccgtggcc ccggtgtgtc gaggtgatgt
     3301 agtcgtccgg gttcagcgct tcgcagacgc cgacgccaac ggcctcctcc ccgcaataca
     3361 gatgcaccga gccgtggatg cgggcctgct ccatgagcag cgcagcccgc tcgtcgaact
     3421 cgcggatccg gaccatggtc tcgagcatgc gaatcatctg ctcttcggga agatccatgg
     3481 cagcgctcct gagaataaga catcgaaggc taacgacgcc cgccaaccag gtcaaacagc
     3541 gccgcaagga gcggccggct cagtcctgga accggattcc ccggtgcatg acggcgcggg
     3601 tagcctccag gtcggtcagg ttcgcatcga gaacgatctc gtccacgccc gcctcggcgt
     3661 aggcggcgat ggtctccgcg atctctctct ccgtgccttt cagcgtccgg cgccgagtca
     3721 ccggtcgatc ccagggttcc cctatgatct cgatgacgca gcgaatgctg atgtcgagat
     3781 ccgccacgtc ccgacccacc gcgcggcacg attcctccag cattttcatc gactccgcca
     3841 gggactccgg gctctgcccg agcgcgtgcc agccatctgc gaaccgcgcg gcgcgccgga
     3901 gtgcgggcgg cgcgccgccg cccaccagga tgggcgggtg cggcgattgc cgtggtttcg
     3961 gctcgaaacg catcccggag aactgataga aggggccttc gtggcgggga aactcctggg
     4021 tccagagcgc cttgatggcg gccagcgcgt cgtccgcgcg ccggccccgg gtagcgaagt
     4081 cgacgccgag gttctcgaac tcgtcctcgg tgactgccac cccgaccccc aggctgaccc
     4141 tgccgtcgga gaggtcgtcc agcgtggcga tggtcttcgc cagccgaacc ggatgatgcc
     4201 agggcagcac gagcacggac gtgcccaggc ggacctgccg ggtggcggcg gcaatggcgg
     4261 tgagcaccgt caggggttca tgatagggct tctcgcccag gcgcctggcc acgtagcctg
     4321 cgtgccagag atgctcgctc acccacaccg acgcgtaacc gagctcctcc gcctcaaccg
     4381 ccagccgcac caggtcggag acgcgctcca cgccctggtt gttcggaatg ctgaatccaa
     4441 gccgcatagc tcgaaacggg cccccagccc tgcctccggt cctgcccgga ttggttcggc
     4501 gagtatttca cgatcgccgg tactcccgca cctcgattag tgctaaaaga aacggcaggc
     4561 agctttggga gaggagcagc ccatgcaggc tgacatcgac gcaaccgccg gacggcacct
     4621 gcgcggcagg tacgccgtgg tgggcgtggg cgagaccggt tacgttcgcg ggtccggctg
     4681 cacgacccgc gccctgggca cccgcgccgt ccgggccgcc ctcgccgatg cagggcttga
     4741 gccaggcgac gtcgacggca tgctgtccta cagctacaac gactcgacct tctctccctt
     4801 cattgccggc gacctgggga tccgtctgaa cttttacatg gacgtctacg gcggcggctc
     4861 gagcatcgag gcgctgatcg ggatcgccat gggcgtcatc gaggcgggca tgtgtcgcac
     4921 ggtggtcatc ttccgggcca tgaacgggta cagccaggta cgtatcggtg gcaccggcgg
     4981 ccgcggcgcg gtggcgccac tcggcggtga cgcgctgttc aaccgggcgt tcggccagat
     5041 gagcgcgggg cagatgttct gccagtcgtt catgcgccac atgtacgact acggcaccac
     5101 gccggagcag gtcgccatgg tcaaggtcgt tcactcggag catgcctcca acaaccccaa
     5161 ggcctactac aggcagcggg tgagcgtgga cgacgtcgtc aacagccgca tgatcgtcaa
     5221 gcccctgcac ctgctggact gctgcgtgga gacggacaac ggcacggccg tcgtggtcac
     5281 cggcatcgac cgcgccaggg actgccggca cacgccggcg gtgatccagt cggtggtggg
     5341 gcgctgcagc aagaggcggt ccgacatgca ctaccagtcg gggccgattt ccaccgtggc
     5401 cggccactac gcgaaggaca tcctctggcc caacgcgggc gtgggcccag aggacatcga
     5461 cgtcaccggt tcctacgatg ctttcacctt caccaccatg ctgcagctcg aggactacgg
     5521 cttctgcaag aagggcgagg gcggcgacta cgtgagctcc gggatcacgc gcctgggtgg
     5581 ggcccggccg aacaacacga gcggcggcca cctgtgcgag ggctataccc acggcatcaa
     5641 catggtgatc gagaacgtcc ggcagctgcg ccacgacgtc gacgacttct gcccgatcgg
     5701 ttccgacggg cgtcgcgtgc acacctacga ctacaccgag ggcggctgcc gccaggtgaa
     5761 ggactgcgag ctgacggcca acctcggctg ggcgacgccc ggtaccggct cggccatggt
     5821 catggcgaga ggaggctgag aatggcgctc gaaggcgagt atctcggcat gcccgtgctg
     5881 gtcgacgacc tggaccagga aaacctggcg ttcttcggcc attgcggccg gggcgagctg
     5941 tgcctgcagc agtgcaaggc ctgcgggctg aagcgttacc cacccaccac ggcgtgcccg
     6001 tggtgcgcga gcccggacgc cacctgggaa ccggtggccg gcaagggcac cgtgtactcc
     6061 tacggcgaga tccaccacgc catccagccc gcattccgcc agaactcccc gtacctgctg
     6121 ctcctggtgg agctcgacga gcagcgcgac ctgccggccc cacacgacgg gctgcgcatg
     6181 acgggcaacc tggcaaccga ggacggcgac ctggcgcccg ccgagctcgt tcgcagcgta
     6241 ggtatcggca gccgcgtgcg ggtagtgttc aaacagctgg gcgacggcat cgcgctgccg
     6301 ctttggtgcc tggacgagtc cgccgagcag ccacagaccc cctggcgtta cccccaggag
     6361 tgatcgccga catctgcccg acgcctccca cacgaaacaa caaggcaagg agagaattca
     6421 atgctcgacc ccaccaccat caccatcttc ggtctggttc tgatcaagct gctgctcgtc
     6481 gccagcatcg gcatgcaccg cctgtacatc gcatcgacgt ctgacaaggc gcccaacacc
     6541 tttctgccca ccggcgagga cgtcggccgg tttgccgtcc ggctggcccg ggcccacgcc
     6601 aactgctacg agtttctgcc cttcgctctg gccgtcctgg tctacgcggc ggccacggaa
     6661 agcagcgcgc tcaccgacgg gctcgcctac tactttctga tcgcacgcat cggacaatcg
     6721 gccacgcacc tggtttcgac gagcacgacg gccgtgttca tccgcttcgc gtttttcctg
     6781 gcgcaggtgg tgattctgct cgtctggtgc ctgggctttc tcggcgcgct ctgagctcgg
     6841 cgaaggcgtc gccggccggg cgcagcgttg agggcatggc acgcatgcta tgctcgggcc
     6901 catgccccag ccggatattc ccatgcctga actgcgagtc ggcgtctgct acgacttccg
     6961 caatccgcct gattccggag tgagcgacgc gcggctgtac gccgaggtca tggagcaggt
     7021 tcgctggctg gacggtatcg gcgccgacct ggtctggttc accgagcacc acttcgtcga
     7081 cgacggctac ctgcccagct ggacgccggt ggccggcgcc atggctgcag tcacggagaa
     7141 cgtgcgcttc ggcaccgaca tctgcctgat gcccttcaac catcccgtcc gcctggccga
     7201 ggatctggcc gtgctcgaca acctgtcggg ggggcgggtc gagctcggca taggcatggg
     7261 ctacgctccc cacgagttcc gcggcttcgg cctgccggta tcacgccggg tctccctgat
     7321 gaacgagggc atagaggtgc tgcaacgctg cttcaccggc gagcgtttca gctatcacgg
     7381 caagcgctac cagctcgacg acgtccgtat cacgccgggg tacgtgcagc ccggcggccc
     7441 gccgttgtgg atcgccgcca tgtccgaagg aggtgcggcg cgggcggccc ggtacgaagc
     7501 caatttcctg ccccagggga acagggcgcg cgtctacgac acgtgggtcg atgctctgga
     7561 aagctccggc cgcagcccgg ccgatcatcg gatcggcatc atccgcagca ttctcgtgac
     7621 cgacgacagg gaaacggact ggcccgtgat ccgtgccgcc gaacgctatc gcatggccct
     7681 gtaccagcgg ttcttcgagg aaagcggcga gggtttcggc gacccgcggg aagccatccc
     7741 ccagacctgg atcgtcggca acgtggatca gtgcgtcgcc gagctgcagc agttcatcag
     7801 cgatttccgg atcaccgaca tcgttaccat ggcagtcccg cccgggctac gcgccgagca
     7861 gatggcgccg agcctggagc gtctgttcac cgaggtggtt cctcgtttga agggaggtcg
     7921 cccatgaggg tcgtcgtcat cggcgcgggc atcggcggct ccgcgctggc gctggcactg
     7981 gatcggctcg gtatcgagca cgtggttctc gagcaggcgc ccgagttcac cgacgtgggc
     8041 gcgggcatcc agctcagccc caacggcgtt cggatcctgg aaagcctcgg cctgcgcgat
     8101 gcgctcaag
//
LOCUS       DBNHOX010000445         3346 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_356607, whole genome shotgun sequence.
ACCESSION   DBNHOX010000445 DBNHOX010000000
VERSION     DBNHOX010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_356607"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="AC16UZ_09920"
     CDS             complement(<1..583)
                     /locus_tag="AC16UZ_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009685946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6247042.1"
                     /translation="MSDTNRQFLLAARPVGMVKESDLQLHEAPVPEPGEGEVLVRVEY
                     ISLDPSMRGQMENRADYVAPLALGDVMRARGVGRVVRSNDPEFPVGAVVSGTFGMQEY
                     AIGSDRVDRFRRWDDAVDPKVVLGTLGGTGLTAYFGLLDVGEPKSGDMVVVSGAAGAT
                     GSVAGQIAKIKGCTVIGMAGTDEKCRWLTDELGF"
     gene            complement(688..1833)
                     /locus_tag="AC16UZ_09925"
     CDS             complement(688..1833)
                     /locus_tag="AC16UZ_09925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6247043.1"
                     /translation="MSDVAIVGIGMHPFGRHEGVTGMEQGASAVREACRDAGVEWKDI
                     QFAFGGSSAAGAPDTMVSQLGLTGLQFINVQNGCATGGSALFSAYNTIMSGAYDLGIA
                     IGFDKHERGAFRIQSGSGKGRDWYGGSGMALTTQFFGMKINHYMQQYGISQSTLAKVA
                     SKAFKNGSMNPMAWRRQALSEEEVLQSQMLSYPLTQYMFCSPGEGGVALILARADQAH
                     KYTKTPVYLKAAVVRSRRFGSFEVLAPSLALEHNAGPTIDASKAAFEIAGIGPEDVDL
                     AQLQDTESGAEVMHMAENGFCEHGEQEHIIQNGETEIGGRFPVNTDGGCLANGEPVGA
                     SGLRQVYENVLQLRGGAGERQVPGNPKVAYTHVYGAPGISGVNILTT"
     gene            complement(1845..2270)
                     /locus_tag="AC16UZ_09930"
     CDS             complement(1845..2270)
                     /locus_tag="AC16UZ_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6247044.1"
                     /translation="MTQVSINDNVLELSAEGPRLKGMRCKTCGNHVFPFQKGCPKCAG
                     TDVEPVDLDTRGTLWAWTIQGFPPKAPPYLGEADPEKFKPYGVGYVEIPGQTKVEARL
                     TESDPTKLKEGMEMELTVEPISKDEDGNDIVTYAFKPVE"
     gene            complement(2287..>3346)
                     /locus_tag="AC16UZ_09935"
     CDS             complement(2287..>3346)
                     /locus_tag="AC16UZ_09935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6247045.1"
                     /translation="DWLRKVREQGAAGFDRDVWRQMAELGWSAIPFADEVGGLGLGYA
                     EAGVVLEELGKALAVTPYLSSVVLGGGAVDLGGSAEQKAEILPGVCDGTTLLTLAYQE
                     TARHDPYAIETSAVKTDGQWVLNGRKQLVLDGGSADRIVVAARSSGAPGDRSGLTLFL
                     VDAGAAGLDVKPGLLLDSRSAAHVALNDVTVGEDAVIGEVDGGAEILDRVFERAAVAL
                     SAEMLGGIQEAFDRTVEYLKEREQFGAKIGSFQGLKHRAARWFCEVELTRSIVMQALR
                     AIDDGAENLAEIVSTCKARASDAFRLSGQEGIQMHGGMGVTDEADIGFFLKRARVSEL
                     LLGDSAFHRNQFAELKGY"
BASE COUNT          541 a         1195 c         1022 g          588 t
ORIGIN      
        1 cgaagccgag ctcgtcggtg agccatcggc atttctcgtc cgtgcccgcc atgccgatga
       61 cggtgcagcc tttgatcttc gcgatctggc ccgccacgct gccggtggcg ccggccgcgc
      121 cgctgacgac caccatgtct ccagacttcg gctcgcccac gtcaagcagg ccgaagtagg
      181 ccgtgagccc tgtgccaccg agggtcccca gcaccacctt ggggtccaca gcgtcgtccc
      241 agcggcggaa acggtccacc ctgtcactgc cgatggcgta ctcctgcatc ccgaacgtgc
      301 cgctcaccac agcgcccacc gggaactccg gatcgttgga ccgcaccacc cggccaaccc
      361 cccgggcccg catgacgtca ccgagagcga gcggggcgac gtagtcggcg cggttctcca
      421 tctgcccgcg catggagggg tccagagaga tgtactccac cctcaccagc acctccccct
      481 cgccgggctc cggaacgggt gcctcgtgga gctgcagatc cgactccttg accatgccca
      541 cgggacgggc ggccagcagg aactgccggt tcgtgtcact catcagcgcc ctcccacggt
      601 ttccagggca gacggtgctg ccccggatgg cccgtggcgc gggtgcttaa gagcgcgtcg
      661 tgcgcgttga ggacagtcat ctttcgacta cgtcgtgagg atgttcaccc cgctgatgcc
      721 gggcgcgccg tacacgtggg tataggcgac cttgggattg ccgggcacct gccgctcgcc
      781 tgcaccgccg cgcagctgaa gaacgttctc gtaaacctgg cgcagacccg aggcccccac
      841 cggctcgccg ttggccaggc agccgccgtc ggtgttcacc gggaaccgcc cgccgatctc
      901 cgtttcgccg ttctgaatga tgtgctcctg ctcgccatgc tcgcagaagc cgttttccgc
      961 catgtgcatg acctcggcgc cggactccgt gtcctgcagc tgggccaggt ccacgtcttc
     1021 cggaccgatg ccggcgatct cgaaggcggc tttggacgca tcgatggtcg gcccggcgtt
     1081 gtgctcgagc gcaagggacg gcgccagcac ctcgaagctg ccgaaacgcc gcgagcgcac
     1141 cacggccgcc ttcaggtaga cgggcgtctt cgtgtacttg tgagcctgat ccgcacgagc
     1201 cagtatcaat gccacgccac cctcgcccgg ggagcagaac atgtactggg tcagcgggta
     1261 ggagagcatc tgggactgca gcacctcctc ctcggacagc gcctggcgcc gccaggccat
     1321 ggggttcatg gagccgttct tgaaggcctt ggaagcgacc ttggcgagcg tgctctgcga
     1381 gatgccgtac tgctgcatgt agtggttgat cttcatgccg aagaactggg tcgtcagcgc
     1441 catgccgctg ccgccgtacc agtcacggcc tttcccggaa ccgctctgaa tcctgaaagc
     1501 cccgcgctcg tgcttgtcga agccgatggc gatcccgaga tcgtaagccc cggacatgat
     1561 ggtgttgtag gcggagaaga gcgccgaccc gccagtcgcg cagccgttct ggacgttgat
     1621 gaactgcaaa cccgtgagac cgagctgcga gaccatcgtg tccggcgcgc cggccgccga
     1681 gctgccgccg aaggcgaact gaatgtcctt ccattcaacg cccgcatccc tgcaagcttc
     1741 gcggaccgct gacgcaccct gctccatccc ggtcacgccc tcgtgccggc cgaatgggtg
     1801 catgcctatg ccaacaattg ctacgtcgct catggtttct ctccttactc gaccggtttg
     1861 aatgcgtagg tgacgatgtc gttaccgtct tcgtctttgc tgatgggctc caccgtcagc
     1921 tccatctcca tgccctcctt gagcttcgtc gggtccgact cggtcaaccg ggcctcgacc
     1981 ttggtctgcc cgggaatctc gacgtagccc acgccatagg gtttgaactt ttccggatcg
     2041 gcttcaccca gatagggagg ggccttgggc ggaaagccct gaatcgtcca ggcccacagc
     2101 gtgccgcggg tgtctagatc cacgggctcc acgtcggtgc ccgcgcactt cgggcagccc
     2161 ttctgaaacg gaaaaacgtg gttgccgcag gtcttgcagc gcatgccttt gagccgcggc
     2221 ccttccgccg acagctccag cacgttgtcg ttgatggaaa cctgggtcat gtcacctcca
     2281 gtcgggctaa tagcccttta actcagcaaa ttggttgcga tggaaggccg agtccccgag
     2341 cagcagctcc gaaacccggg cgcgcttcaa gaagaagccg atgtccgcct cgtccgtcac
     2401 gcccatgccg ccgtgcatct gtatcccttc ctggcccgaa agccggaagg catccgaagc
     2461 cctcgccttg caggtgctca cgatctcggc gaggttttca gcgccgtcat cgatggcgcg
     2521 cagcgcctgc atgacgatgg agcgggtcag ctccacctca cagaaccagc gggccgcgcg
     2581 gtgcttcaag ccctgaaagg aaccgatctt cgcgccgaac tgctcgcgtt ccttgagata
     2641 ctcgacggtc cggtcgaagg cttcctggat gcctccgagc atctcggccg agagcgccac
     2701 cgccgcgcgc tcgaagaccc ggtcgagaat ctcggcacca ccgtcgacct cgccgatcac
     2761 ggcatcctcg cccacggtca cgtcgttcag ggccacgtga gccgcgctgc ggctgtcgag
     2821 cagcaggccc ggcttcacgt cgagcccggc ggcgcccgcg tcgaccagga acagggtcag
     2881 ccccgaccga tcgccgggcg ccccgctcga ccttgccgcg accacgatcc ggtccgcgct
     2941 gccgccgtcc agaaccagct gcttgcggcc gttgagcacc cactggccat cggttttcac
     3001 ggcgctcgtc tcgatggcgt agggatcgtg acgggcggtc tcctggtagg cgagcgtcag
     3061 cagcgtcgtg ccatcgcaga cgccgggaag gatctcggcc ttctgctcgg cactgccgcc
     3121 gaggtctacg gcgccgccgc ctagcaccac gctggacagg tagggcgtca ccgccagcgc
     3181 cttgccgagc tcctcgagca ccacgccggc ctccgcgtag ccgaggccga gcccgccgac
     3241 ctcatcggca aacgggatcg ccgaccagcc gagctcggcc atctgccgcc agacgtcccg
     3301 atcgaaaccg gcggcgccct gttcccgcac tttccgcaac cagtcg
//
LOCUS       DBNHOX010000446         4567 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1384711, whole genome shotgun sequence.
ACCESSION   DBNHOX010000446 DBNHOX010000000
VERSION     DBNHOX010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1384711"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="AC16UZ_09940"
     CDS             complement(<1..338)
                     /locus_tag="AC16UZ_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247046.1"
                     /translation="MTVESFDPSAVDHTISDSRLARLLDAAGCLDEESFGLGTEEVRD
                     LAAVARHPESDWSERAGTLGDEEVLALIRLFTLAETRLSGWESGAASPVVPLAAALKR
                     RDAYPSDLNR"
     gene            450..1964
                     /locus_tag="AC16UZ_09945"
     CDS             450..1964
                     /locus_tag="AC16UZ_09945"
                     /inference="COORDINATES: protein motif:HMM:NF017021.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR6247047.1"
                     /translation="MVKRILMILLWIVLLGGGFVFYWIQDANRIKPELEQLIEEQAGI
                     PVTIGGDLSWRLFPPLTLQAEQIAASYEGQDWTAERLSLDIDVMTVLRTRDVNRWRVQ
                     SLTLTNTVGRDARTEIDIKSLTLREFELGKPSPLKISMVYTGEGAEPLPVKMTGLVTV
                     QSGPDRYEITNADLSTPYADGVCNLEASPASSSAPLPPATLEDLIPVATWRAFDWSGR
                     CDLSRLALDDLTFDSATVDLANTAANSATRITIPEFFGGTAILELQIDASGTPVRWIL
                     TPDLTGVDSQRLMAWLDQRLEWMAPLAYNGTLKFEGNTADALIASLSGETRFDGGRGA
                     ISIIKIKQPLLAVATMLQEPGRVSGWPDLWQYDRLEGVWRADRQHHTLDFALDNLSVI
                     ADGNYDPAADRLDMMAELTFRSLTEGKMFELNPVLMDLPIPVRCRGSLENPECRVDQK
                     AAQRIVASVLTSKEGNEAREKLEEKIDEEVPEEYRDAARGLLDLLGRSLQDKNQ"
     gene            1967..3034
                     /gene="mutY"
                     /locus_tag="AC16UZ_09950"
     CDS             1967..3034
                     /gene="mutY"
                     /locus_tag="AC16UZ_09950"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR6247048.1"
                     /translation="MRPDWFTKRVLKWFDLHGRHDLPWQEDNDPYRVWVSEIMLQQTQ
                     VTTVIPFYERFIARFPTVAALAGAELDEVLHHWTGLGYYARGRNLHKAARVIAHDLDG
                     RFPVGVEALSELPGIGRSTAGAISAIAQGIRAPILDGNVKRVLARFHAVAGYPGQTAT
                     QKSLWELAEAHTPNTRVADYTQAIMDLGATVCTRQPRCEVCPVAERCDALAQGATDDF
                     PGRKPKRSKPTRTARMFLIVAPDGACLLEQRPAKGLWGGLWIPPQRGPDCDVPAVLRE
                     FDIPESAVAESRVEPGFRHTFTHFHLDIEPVHVRLRARPLPGVRDGTDCLWYRREDDT
                     EVGLSAPAVALLDSLKEFELA"
     gene            3031..3303
                     /locus_tag="AC16UZ_09955"
     CDS             3031..3303
                     /locus_tag="AC16UZ_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HIR6247049.1"
                     /translation="MSRTVTCRKYGEELEGLDAPPMPGPKGQEIFETVSRRAWQEWQD
                     LQTMLINERALDMRDPEARKYLNAQRERFLDNEETDHAEGHVPRSS"
     gene            3284..4204
                     /locus_tag="AC16UZ_09960"
     CDS             3284..4204
                     /locus_tag="AC16UZ_09960"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR6247050.1"
                     /translation="MCPDLPEQQTMPMGRPQAERVVDEALARRLLEDQHPDLADLPLT
                     HLDSGWDNVIYRLGDKLTVRLPRREVAAALLHHEQTWLPRIADGLPLPVPSPVAMGRP
                     TAYYPWPWSVLPWFEGESADLSPPRAEQADRFADFLIALHRPAPADAPANPVRGVPLM
                     AREANTRERMGRLNSKSDALSPALESIWESALTAPQAGEACWLHGDLHAQNVLVNGTG
                     SLTAIIDWGDITRGDPATDLAGIWAVFPTRRARQSILQAYRPDTALLDRARGWAFVFG
                     VILTDSGNVNSPRHAAAGRRILERLEADSG"
     gene            4322..4396
                     /locus_tag="AC16UZ_09965"
     tRNA            4322..4396
                     /locus_tag="AC16UZ_09965"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4354..4356,aa:Phe,seq:gaa)
     gene            4416..4488
                     /locus_tag="AC16UZ_09970"
     tRNA            4416..4488
                     /locus_tag="AC16UZ_09970"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4449..4451,aa:Phe,seq:gaa)
BASE COUNT          833 a         1538 c         1437 g          759 t
ORIGIN      
        1 caccggttga gatctgacgg gtaggcatcg cggcgcttga gcgccgccgc cagcgggacc
       61 acgggcgatg ccgctccgga ctcccagccc gacaggcggg tttccgccag cgtgaacagc
      121 ctgatcaggg ccaggacttc ctcatctccc agcgtccccg cccgctcgga ccagtccgac
      181 tccggatggc gggcaacggc cgcgagatct ctcacctcct cggtaccgag accgaagctc
      241 tcctcgtcga ggcaaccggc cgcatcgagc aggcgcgcga gacgcgaatc ggaaatcgtg
      301 tggtccacgg ctgaggggtc gaaggattct acggtcatgg cggccagatg gtctgcgggg
      361 gcgagactat agcaaacgcg ccgttcagct gccggccagc ccgaaatcct tataatcgtc
      421 ctcacttccc agactcacgg caacaccaca tggtcaaacg cattctcatg atcctcctgt
      481 ggatcgtgct gctcggtggc ggcttcgtct tttactggat ccaggacgcc aaccggatca
      541 agccggagct ggagcagctc atcgaagagc aggccggcat ccccgtcacc atcggcgggg
      601 atctgagctg gcgtctgttc ccgcccctga cactgcaggc agagcagatc gccgcgagct
      661 acgagggtca ggactggacg gctgaaaggc tcagcctgga catcgacgtc atgaccgtgc
      721 tgcgtacccg ggatgtgaac cgctggcggg tgcagtcgct gactttgacg aacaccgtcg
      781 gccgcgacgc ccggacggag atagacatca aatccctcac cctgcgggaa ttcgagctgg
      841 ggaagccgtc gccgctgaag atctcgatgg tctataccgg ggaaggagcc gagccgctgc
      901 ccgtgaagat gaccgggctc gttaccgtcc agtccggacc cgaccgatac gagatcacta
      961 acgcggatct atccaccccc tacgccgacg gcgtctgcaa cctcgaggca agcccggcca
     1021 gcagcagcgc cccgctccca ccggcgaccc tcgaggatct cattcccgtc gccacatggc
     1081 gcgccttcga ctggtccggc cgatgcgacc tgtcgcgcct cgcgctcgac gacctcacct
     1141 tcgactccgc cacggtcgat ctcgccaaca ccgctgcaaa cagcgccacg cggatcacca
     1201 ttcccgagtt cttcggcggc acggccatcc tcgagctgca gatcgacgcc agcggcacgc
     1261 ccgttcgctg gatcctgacc cccgacctta ccggggtgga cagccaacgt ctcatggcct
     1321 ggctggatca gcgcctggag tggatggcgc cgctggccta caacggcacc ctgaagttcg
     1381 aaggcaacac ggctgacgcg ctcatcgcct cgctttccgg cgagacgcgt ttcgacggtg
     1441 ggcggggcgc catctccatc atcaagatca agcagccgct tctggctgtc gccaccatgc
     1501 tgcaggagcc gggccgggtc tccggatggc ccgatctgtg gcaatacgat cgtctcgagg
     1561 gcgtgtggcg ggcggaccgg cagcaccaca ccctggactt cgctctggac aatctgtcgg
     1621 tcatcgcaga cggcaactac gatcctgccg ccgatcggct cgacatgatg gcggagctga
     1681 ccttcaggtc gctgaccgaa ggaaagatgt tcgagctgaa cccggtcctg atggacctgc
     1741 ccattcccgt tcgctgtcgc ggcagcctgg aaaaccccga atgtcgggta gatcagaaag
     1801 ccgcccagcg catcgtggcc tccgtgctga cgagcaaaga gggcaacgag gcgagagaga
     1861 agctcgagga gaagatcgac gaagaggtcc ctgaggagta ccgggacgca gcccgcggcc
     1921 tgctggatct cctgggcaga tcattgcagg acaagaacca gtaacggtga ggcccgactg
     1981 gttcacgaaa cgcgttctga agtggttcga cctgcacggc cggcacgacc tgccgtggca
     2041 ggaggacaac gatccgtacc gggtatgggt ctccgagatc atgcttcagc agacccaggt
     2101 caccaccgtg atcccgttct acgagcgttt cattgcccgt ttcccgacgg tcgcagcact
     2161 cgctggggcg gagctggacg aggtcctgca ccactggacg gggctcggct actacgcaag
     2221 gggccgcaac ctccacaagg ccgcccgggt catcgcgcac gatctcgacg gccggtttcc
     2281 cgtcggggtc gaggcgctga gcgagctgcc gggaatcggt cgttctaccg ccggcgccat
     2341 ctccgcaatc gcccagggaa tccgcgcgcc gattctcgac ggcaacgtca aacgggtgct
     2401 agcccgcttc cacgccgtgg caggctaccc cggccagacg gccacgcaga agtcgctctg
     2461 ggagctcgcc gaggcccaca ccccgaacac gcgtgtcgcc gattacaccc aggcgatcat
     2521 ggacctcggc gctaccgtgt gcactcgcca gccccggtgc gaggtctgcc ccgtggccga
     2581 gcgctgcgat gccctcgccc agggcgcaac cgatgacttt cccgggcgca agccgaaacg
     2641 gtccaagccg acgcgcacgg cgagaatgtt tcttatcgtc gccccagacg gggcgtgcct
     2701 gttggagcag cgaccggcca agggcctgtg gggcggattg tggatcccgc cgcagcgggg
     2761 ccccgactgc gacgttccag cggtgctccg ggagttcgac atcccggaat ccgctgttgc
     2821 cgaaagccgg gtcgagcctg ggtttcgcca cacgttcacc cactttcatc tcgacatcga
     2881 gcccgtgcac gtccgccttc gcgcgagacc gcttcccggc gttcgcgacg gcacggactg
     2941 cctgtggtac cggagagaag acgacaccga ggttggactg tccgctcccg cggttgcact
     3001 attggattcc cttaaggagt ttgagctggc atgagccgca cggtcacctg ccgcaagtac
     3061 ggcgaagagc tcgagggtct cgacgcgccg cctatgcccg gacccaaggg ccaggagatc
     3121 ttcgaaaccg tctcgcgcag agcctggcag gagtggcagg acctgcagac gatgctgatc
     3181 aacgagcgag cgctggacat gcgcgacccc gaagcaagaa agtatctcaa cgcccagcgg
     3241 gaacggtttc tggacaacga ggagaccgac cacgccgagg gccatgtgcc ccgatcttcc
     3301 tgagcagcag accatgccca tggggcggcc ccaggcagag cgggtcgtgg acgaggcttt
     3361 ggcgcgacgg ctgctggagg accagcaccc ggacctcgcc gatctgccgc tcacgcatct
     3421 ggactccggc tgggacaacg tgatctaccg gctgggcgat aagctcacgg tgcgcctgcc
     3481 ccggcgggag gtggctgcgg cgctgctcca ccacgagcag acctggttgc cccgcatcgc
     3541 agacggcctt ccccttcccg tgccgagccc ggtcgccatg ggcagaccga cagcctacta
     3601 cccctggccc tggagcgttc tgccctggtt cgagggggag agcgccgatt tgtcgccgcc
     3661 gcgcgccgag caggccgacc gctttgccga ctttctcatc gccctgcacc gccccgctcc
     3721 ggcagacgct ccggctaatc cggtccgggg ggtaccgctc atggcgcgcg aggccaatac
     3781 ccgcgagcgt atggggcgcc tgaactcgaa aagcgacgcg ctctcccccg ccctcgaatc
     3841 gatctgggag tcggcgctca cagcgccgca ggccggcgag gcatgctggc ttcacggcga
     3901 cctgcacgct cagaacgtgc tggtgaacgg caccggcagc cttacggcaa tcatcgactg
     3961 gggagacatc acccggggcg atccggccac cgatctggcc ggcatctggg ccgttttccc
     4021 cacgcgccgt gcgcggcaga gcattctcca ggcatatcgg ccggataccg cactgctcga
     4081 ccgagctagg gggtgggcct tcgtattcgg cgtcatcctg accgattcgg ggaacgtcaa
     4141 cagcccccgg cacgctgccg ccggcaggcg aatcctcgag cggctggagg cggattccgg
     4201 ctagccgcga cgccttgcag tttaagggtc agatcggttt aataccgccc ctttgctggg
     4261 gaccaaacaa gaaagcctgt cccaggacgc agtgaaaggg cccgccccag caagctttcc
     4321 ggccaggtag ctcagttggt agagcagcgg actgaaaatc cgcgtgtcgg cagttcgatt
     4381 ctgccccttg gcaccatttt tttattcgat acttcgctgg agtagctcag tcggtagagc
     4441 agcggactga aaatccgcgt gtcggcagtt cgattctgtc cctcggcact caatcaaata
     4501 aagggtttag ctgatgccgg ttaaaccctt tatctgtttc aggcttgtca gaggggtatg
     4561 gttagta
//
LOCUS       DBNHOX010000447         3637 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1513950, whole genome shotgun sequence.
ACCESSION   DBNHOX010000447 DBNHOX010000000
VERSION     DBNHOX010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1513950"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1098)
                     /locus_tag="AC16UZ_09975"
     CDS             complement(160..1098)
                     /locus_tag="AC16UZ_09975"
                     /inference="COORDINATES: protein motif:HMM:NF016241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HIR6247051.1"
                     /translation="MHLKNGDRLSGTVDSIDGKRVVLDTDVAGKVVLKLSSVLNIETE
                     ESFDVKLKSGTKATGRFAVDDGAQTIEAQEEAPLPVALEEIRSAGQDRIAFVNLGSDW
                     STRLDLAAAISSGNSETESFNTVLESKLTRGRSEHGLYARISSEESENEKTKDQLDVK
                     YGYKRFFAEKWFGSGNVEYFEDGLKGIDHRITTGAGVGYQFWDDSFGALSVEAGGNWV
                     FEELFGEKEDNPAFRWALKFNRHLWSKRVEVFHNHSVLVIPDRGEVFDSSTGLRLAMT
                     DRVDSNFRVDLQHETDPPDGSEKTDVTYSIGVGVKF"
     gene            complement(1198..2085)
                     /locus_tag="AC16UZ_09980"
     CDS             complement(1198..2085)
                     /locus_tag="AC16UZ_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6247052.1"
                     /translation="MSRILEELVADARELARRALQLVPAAPSVDWENTLAASWRETSW
                     GSGFAAHSELDDIELDDLLCVDDQKRSLDLNTQQFLAGAPANNVLLWGARGTGKSSLI
                     HALLNHYHARGLRLVEVSKTRLSGLPEIVLRLAAEPYRFVLFCDDLSFEADDASYKEL
                     KSALEGSVFKASSNVLIYATSNRRHLLPEYMSDNAQSGFVEGELHQSEAVEEKISLSD
                     RFGLWLSFYPFKQDAYLDVVRHWLGRLASEHDAEFVWDDEVRAEALRWALARGVRSGR
                     TAQHFARHWVGQLIINSNQ"
     gene            2254..2400
                     /locus_tag="AC16UZ_09985"
     CDS             2254..2400
                     /locus_tag="AC16UZ_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247053.1"
                     /translation="MGERTGEDQRKGPKDRRTAAQDRRNAERMAEDLNPRRDPERRGR
                     RTGD"
     gene            complement(2421..2825)
                     /locus_tag="AC16UZ_09990"
     CDS             complement(2421..2825)
                     /locus_tag="AC16UZ_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247054.1"
                     /translation="MGSLLVVIAIACTTVLWVKGARKNRQRWLARLDLPGTWRWRPSD
                     SAESEVQGSLELSGGLSSGAYRLVEADVEEAGNWALEGHTLLLSPTGAASTGHYEIRF
                     FDEGKIGIDGPGRERRIYVKERTNVVPLRRRG"
     gene            complement(2829..>3637)
                     /locus_tag="AC16UZ_09995"
     CDS             complement(2829..>3637)
                     /locus_tag="AC16UZ_09995"
                     /inference="COORDINATES: protein motif:HMM:NF022723.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3080 family protein"
                     /protein_id="HIR6247055.1"
                     /translation="IDVLDFIELHDCDLAGVVGFRNSPLGRLQTPSQRLGYESEWLAG
                     ARSCASDGVGWLEPLIAKKEAALPAVFWNATFAGEEFARLLGRFEASETPGAGYLLRE
                     LGDAYATLATDRFDLGGFERVLGALGSIPWLGQARRAWDLERRHLDAVAGALTEGLEG
                     VCRNGAPTPKSRALVNVFQRYYVDALQPGIARRHGTDRDAVAAVAALVGLFGGDVPGE
                     FAVWYGQVLAPEPRRSEWSRLASVRLEHAAAWNRLFARCGVDPARGLAQH"
BASE COUNT          687 a         1243 c         1082 g          625 t
ORIGIN      
        1 taggggagtc gaacccctgt tgccaggatg aaatcctgga gtcctaggcc tctagacgaa
       61 ggggacttag aaggcgcgga attctaagaa gccgccctac cccagtcaag caatgccggc
      121 atgccaaggc aatctccccg ccggcaccca tctgcccgct cagaacttga ccccaacgcc
      181 gatgctgtag gtcacgtccg tcttttcgct tccgtccggc ggatcggttt cgtgctgaag
      241 atcgacccgg aagttgctgt ccacccggtc ggtcatcgcc aggcgcagtc ccgtggacga
      301 gtcgaagacc tcaccgcggt cggggatgac gagcaccgag tgattgtgga agacctccac
      361 ccgcttgctc cagaggtggc gattgaattt cagcgcccag cggaaggcgg ggttgtcctc
      421 tttctcgcca aacagctcct cgaacaccca gttgccgccg gcttccacgg agagcgcgcc
      481 gaagctgtcg tcccagaact ggtaacccac gccggcaccc gtggttatcc ggtgatcgat
      541 gcccttcaga ccatcctcga agtactccac gttgccggag ccgaaccact tctcggcaaa
      601 gaaccgcttg tagccgtatt tgacgtccag ctgatccttc gtcttctcgt tctccgactc
      661 ctcgctgctt atgcgggcat agaggccgtg ctcagagcgg ccgcgcgtca gcttggactc
      721 gagcaccgtg ttgaacgact cggtttccga gttaccggag gatatggcag ccgcgagatc
      781 gagccgggtg gaccagtcgc tgccgagatt gacgaacgcg atccgatcct gacccgcgga
      841 cctgatttcc tcgagagcca cgggcaaagg cgcctcctcc tgcgcctcga tggtctgggc
      901 cccgtcgtcc acggcgaagc gcccggtcgc tttcgtcccg ctcttgagct tcacgtcgaa
      961 gctctcctcg gtctcgatgt tgagcaccga gctcagcttg agcaccacct tgccggccac
     1021 gtcggtgtcc agaacgacgc gcttgccgtc gatgctgtct accgtcccgc tcagccggtc
     1081 gccatttttc agatgcacga cgtcggctcc cgccggtgcc gtccccagaa ggctgagtat
     1141 ccccaaggca atagctgccc ggatctttcg atcttctaga aggttcacac gatcccctta
     1201 ttgattgctg ttgatgatga gctggccgac ccagtggcgc gcaaagtgct gcgcggttcg
     1261 gccacttctc actccgcgag cgagagccca gcgcagggct tctgctctga cctcgtcgtc
     1321 ccacacgaat tcggcgtcgt gctcacttgc gagcctgccg agccagtgcc tgacgacatc
     1381 gagataggcg tcctgtttga aaggataaaa ggacaaccac aacccgaagc gatcggatag
     1441 agagattttt tcttcgaccg cctcggactg atgcagttcg ccttccacga aaccgctctg
     1501 ggcgttgtcc gacatgtact cgggaagcag gtgccggcgg ttggaggtgg cgtagatcag
     1561 gacgttgctc gacgccttga aaaccgatcc ttccagagcg ctcttgagtt ccttgtagct
     1621 ggcatcatcc gcctcgaagg agaggtcgtc acagaacagc acgaagcggt agggctctgc
     1681 agccagtcgc aggacgatct ccggcaggcc ggacaggcgc gtcttgctga cttcgacgag
     1741 cctcaatccc cgtgcgtgat agtgattcag gagcgcatgg atgagcgacg acttaccggt
     1801 gccgcgggca ccccacagca gcacgttgtt ggccggggca ccggcgagaa actgttgcgt
     1861 gttgagatcc agcgaccgct tctgatcgtc cacgcagaga agatcgtcca gctcaatgtc
     1921 gtccagctcg gaatgtgcag caaatcccga gccccatgac gtctcccgcc agctggcggc
     1981 cagggtgttt tcccaatcca cggacggcgc cgccgggacg agctgcagcg cccggcgagc
     2041 cagctcgcgg gcgtcggcga cgagttcctc caggattctg ctcacgaaaa gtccagggtt
     2101 tcaagcgggc gcaattgtaa ccagaaaccg accggaaact gaccacgtgc ggtgaggcat
     2161 gtatctatcc atgttagact tcttcggggt cgcgcccgga atgaacgggc ccattccaag
     2221 caagcacaac cagagccatc gaactgcgca aacatgggcg aacgcaccgg agaagatcag
     2281 cgtaagggac caaaggatcg acgcacggcg gcccaggacc gtcgcaacgc cgagcgcatg
     2341 gccgaggacc tcaatccgcg gcgcgacccg gagcggcggg gccgccggac gggcgactag
     2401 acgaacgggc ttgccttgct tcagccgcgc ctgcggagcg gcaccacgtt ggtccgctcc
     2461 ttgacgtaga ttcgccgctc ccggccgggc ccatcgattc cgatcttgcc ctcgtcgaaa
     2521 aagcggattt cgtagtgccc cgtgctcgcg gccccggtcg ggctcaaaag cagggtatgt
     2581 ccctctaaag cccagttccc ggcctcttcg acatcggctt ccaccagccg ataagctcca
     2641 gagctcaggc caccgctcag ctcgaggctg ccctgaacct ccgattcggc ggaatcagac
     2701 ggccgccagc gccacgtacc gggcagatcc aggcgcgcaa gccagcgctg ccggttcttc
     2761 cgggcacctt tcacccagag cacggtagtg caggcgatgg caatgacgac cagcagagaa
     2821 cccatggctc aatgctgcgc caagccccgc gccggatcaa ctccacagcg cgcaaagagc
     2881 ctgttccagg cagcggcgtg ctcaaggcgc accgacgcga gtcgggacca ttcactccgc
     2941 cgaggctcgg gggcgagcac ctgtccgtac cagacggcga attcgccggg cacgtcgcca
     3001 ccgaagagcc cgacgagcgc ggcaactgca gcaacggcat cccggtcggt cccatgccgc
     3061 cgcgcgatgc ccggctgcag ggcgtcgacg tagtaacgct ggaagacgtt tacgagtgcc
     3121 cgactcttcg gcgtcggggc gccgtttcga cacacccctt cgagcccctc ggtcagcgcc
     3181 ccggccaccg cgtccagatg acgcctctcc agatcccacg cccgtctggc ctggccaagc
     3241 caggggattg agccgagcgc tccgagcacc cgctcgaacc cgccgaggtc gaaccggtcg
     3301 gtggcgagcg tcgcgtaggc gtcccccagt tcccgcagca ggtagcccgc ccccggcgtc
     3361 tcggacgcct cgaacctacc cagcagccgg gcgaactcct cccccgcaaa cgtggcgttc
     3421 cagaagacgg ccggaagcgc agcctccttc ttcgcgatca gcggctccag ccaccccacc
     3481 ccgtcggacg cgcaggaccg agcgcccgca agccactcag actcgtagcc caggcgctgg
     3541 ctcggcgtct gcaaacgccc cagcgggctg ttgcgaaacc ccaccacccc ggccaggtcg
     3601 cagtcgtgca gctcgataaa gtccagaacg tcgatgc
//
LOCUS       DBNHOX010000448         1603 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_869889, whole genome shotgun sequence.
ACCESSION   DBNHOX010000448 DBNHOX010000000
VERSION     DBNHOX010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_869889"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="AC16UZ_10000"
     CDS             complement(<1..710)
                     /locus_tag="AC16UZ_10000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6247056.1"
                     /translation="MAGFAYVAPSSIDEAVAVLAEHAGQGLKAQVIAGGTDLLVQMRS
                     LNKAARTIVDIKKIGETGRLDIGADETFIGAGICCAALHESDELKALFPGLLESADLI
                     GSTQIQGRATLGGNLCNASPAGDTIPAMIANAGVCVIAGPGGRRELPVEDFVVGVGRN
                     ALQDGEFLVGLKFPNPKAATADAYLRFIPRTEMDIAVAGAGVRITLDADGTCTEARVA
                     IGAVAPTAILVPAAASALV"
     gene            811..>1603
                     /locus_tag="AC16UZ_10005"
     CDS             811..>1603
                     /locus_tag="AC16UZ_10005"
                     /inference="COORDINATES: protein motif:HMM:NF044430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR6247057.1"
                     /translation="MTVTEPSLDTPLFAEHVGELSRRWSDALTASAFDGALIAAGRAR
                     TYFLDDQAPTFRANPHLAQWLGDDDCEGSLILITADGQAQLFFHQPDDYWHQPPKAPD
                     HLNAFMSIEVFPDPDTLADAAMTRAQQLNRVAFIGEDLPGNLPVAESNPAQLVNRIHY
                     QRAYKTGFELACMREATRKAVLGHKAAGEAFHGGASEFEIHLRYLEASEQTEADVPYP
                     NIVALNEHAGVLHYQHYDRQPPAARHSFLIDAGARHRCYASDVTRT"
BASE COUNT          292 a          559 c          485 g          267 t
ORIGIN      
        1 accagggcgc tggccgcggc tggaaccaaa atggccgtgg gcgcgacggc acctatggcc
       61 actcgcgctt ccgtgcaggt cccgtcggcg tccagcgtga tccgcacgcc ggcccccgcc
      121 acggcgatgt ccatctcggt gcgcggaatg aaccgcaggt acgcatccgc agtggctgcc
      181 ttggggttgg gaaacttgag gcccaccaga aactcgccgt cctgcagcgc gttgcggccc
      241 acaccgacga cgaaatcctc gaccggcagc tcgcgacgcc caccgggtcc ggctatcacg
      301 cagacgcctg cgttggcgat cattgccgga atggtgtccc ccgcagggga ggcgttgcac
      361 aggttccccc cgagggtcgc gcgtccctgg atctgggtgg agccgatcag gtcggcggat
      421 tcgagcagcc cgggaaacag ggctttgagc tcgtcgcttt cgtgcagcgc tgcgcagcag
      481 atgccggcgc cgatgaacgt ctcgtcggcg ccaatgtcca ggcgtccggt ctcgccgatc
      541 ttcttgatat cgacgatcgt ccgagcggcc ttgttcagcg atcgcatctg cacgagcaga
      601 tcggtgccgc ccgctatgac ctgggccttc agcccctgcc ctgcatgctc ggccagcacg
      661 gccaccgcct cgtcaatgct ggacggcgcc acgtaggcaa atcctgccat gagtcctccg
      721 gaaagtgcgt ttgctcagca gagcaatcta tcacgagttc aactgcgggt ggagagcaat
      781 gcgagcagtt cacgtatact tgcgcccgca atgaccgtca cggaacccag cctggacact
      841 ccgctgttcg ccgagcatgt tggcgagctc tcgcggcgct ggtccgatgc gctgacggcg
      901 tccgcgttcg atggcgcgct catcgcagcc ggccgtgcaa ggacctattt cctcgacgac
      961 caggcaccga ctttccgggc caacccccat ctggcgcagt ggctgggcga cgacgactgc
     1021 gaggggagcc tgatcctgat caccgcggac ggacaagcgc agctcttctt tcatcaaccc
     1081 gacgactact ggcaccagcc tcccaaagcc cccgaccatc tgaacgcgtt catgtccatc
     1141 gaggtctttc ccgacccgga taccctcgcc gacgccgcca tgacccgggc gcagcagctc
     1201 aaccgcgtag ccttcatcgg cgaagatctg cccggaaacc tgccggtcgc cgagtcaaac
     1261 cccgcacagc tggttaatcg aattcattac cagcgcgcct acaagaccgg tttcgaactg
     1321 gcctgcatgc gcgaggcgac gcgaaaggcc gtattggggc acaaggcggc cggcgaagct
     1381 ttccatggcg gcgccagcga gttcgaaatt cacctgcgat acctcgaagc atccgagcag
     1441 acagaggcgg acgtgcccta tccgaacatc gtggcgctga acgagcacgc gggagtcctg
     1501 cactaccagc actacgaccg gcaaccaccg gctgcccgcc acagcttcct catcgatgcc
     1561 ggcgcccggc atcggtgtta cgccagcgac gtgacacgga cct
//
LOCUS       DBNHOX010000449         2093 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_228276, whole genome shotgun sequence.
ACCESSION   DBNHOX010000449 DBNHOX010000000
VERSION     DBNHOX010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_228276"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="AC16UZ_10010"
     CDS             <1..581
                     /locus_tag="AC16UZ_10010"
                     /inference="COORDINATES: protein motif:HMM:NF025134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HIR6247058.1"
                     /translation="AFEPSDGFESIADFNAALAAHIKTHPTLQGNVMSTEHGEHTGEL
                     LREPVGPMGAMEIRIHEAVRWYIGQLPDDPQHPAVRWVPKAWKLTTWGVVMFDRGHER
                     AHIHPNGWLSGVFYLELPDLIADPARKPEGWLEFGRPTTDLHVQSELTLRHYQPAYGQ
                     MFLFPSYFYHGTVPFRSDQERVCVAFDVEPLY"
     gene            complement(597..1238)
                     /locus_tag="AC16UZ_10015"
     CDS             complement(597..1238)
                     /locus_tag="AC16UZ_10015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6247059.1"
                     /translation="MSEEPEDVQIHLDSVDHVLSTARSVRRKLDFSRPVDPEVIFDCI
                     NVSTQAPTGLGGESWRFLVVTDLGRKREIAGLYRDVLVGLQQERGLVIKPTQQALVDR
                     LHEIPAMIFVCSMGAPPGPEIPTQVAYYGSVLPSAWSLMLSLRARNLGATWTTLLSSQ
                     QEALGRILDVPEDVVFTVMLPVGYMKGARLKKADRMDARRVTFWNRWGNDAPH"
     gene            1339..>2093
                     /locus_tag="AC16UZ_10020"
     CDS             1339..>2093
                     /locus_tag="AC16UZ_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR6247060.1"
                     /translation="MSELTHLFQPIQIGTMNLRNRIMMPPHGGFIGDLWGSEEAAEQN
                     LAYWARRARDGVAWIDGITGFVDNTLMVPGFLPTGLGAITRGVFRMPHFKERAGRYAD
                     VIHEGGACATSQLVLQGGMPHGPSARLANYTNNSVPHVLDKDEIRWLVEEYAYSARQI
                     ADAGLDGLELHANHEDLLELFLSPATNHREDEYGGDLNGRLRFIKEILEAIRAEVGNS
                     ITVGVRLNMDEFFEGGYDLDEGLEIAKGLDATG"
BASE COUNT          380 a          706 c          677 g          330 t
ORIGIN      
        1 acgcattcga gccgtcagac ggcttcgaga gcatcgctga cttcaacgcc gcgctggcag
       61 cccacatcaa aacccacccc accctgcagg ggaacgtgat gtcgaccgag cacggcgagc
      121 acacgggcga gctgctgcgc gagccggtgg ggcccatggg agccatggag atccggatcc
      181 acgaggccgt gcgctggtac atcggccagc tgccggacga tccgcagcat cccgccgtgc
      241 gctgggtgcc gaaagcctgg aagctgacca cctggggcgt cgtgatgttc gaccgcggcc
      301 acgagcgcgc ccacattcac cccaacggct ggctgagcgg cgtgttctac ctcgagctgc
      361 cggatctgat tgccgaccca gcccgaaagc ccgaaggctg gctggagttc ggccgcccta
      421 cgaccgatct gcacgtgcag tccgagctga ccctgcgcca ctaccagccg gcctacggcc
      481 agatgtttct gttcccgtcc tacttctatc acgggaccgt tccgttccgg agcgatcagg
      541 agcgcgtctg cgtggccttc gacgtggagc cgctctactg atccagcaac agcgcttcaa
      601 tgcggcgcgt cgttgcccca gcgattccag aaggtaaccc gtcgggcgtc catccggtcg
      661 gccttcttga ggcgagcccc cttcatgtag cccaccggca gcatgacggt gaagaccacg
      721 tcttcgggaa cgtcgaggat gcggccgagc gcttcctgct gcgaggagag cagcgtggtc
      781 caggtggcgc ccaggttgcg ggcgcgaaga gacagcatca gcgaccacgc cgagggcagc
      841 accgagccgt agtaggccac ctgggtaggt atttccggcc ccggcggcgc gcccatggag
      901 cagacgaaga tcatggccgg aatctcgtgc agccgatcca ccagggcctg ctgggtgggc
      961 ttgatgacga gcccccgctc ctgctgaagc cccaccagca cgtcccggta aaggccggcg
     1021 atttcccgct tccggcccaa gtccgtcacc accaggaagc gccagctttc gccgccaagg
     1081 ccggtgggcg cctgggtgga gacgttgatg cagtcgaaga tcacctccgg gtccaccgga
     1141 cgggaaaagt cgagcttgcg ccgcaccgag cgcgccgtgc tcagcacgtg atccacgctg
     1201 tcgagatgga tctgaacgtc ttcgggctct tcggacatga ggcgacctcc gcgatccatc
     1261 gtgccacgcg ccggggttgt aatcgagccg caattggtga ataacgaacc gctgggggat
     1321 acggtcgaag gggaggccat gagcgagctc acacacctgt ttcagccgat tcagatcggc
     1381 accatgaacc tgcgcaaccg catcatgatg ccaccgcacg gcgggttcat cggcgacctc
     1441 tggggcagcg aggaagccgc cgagcagaac ctggcctact gggcccgacg ggcccgcgac
     1501 ggggtcgcct ggatcgacgg gatcacgggt ttcgtggaca acacgctgat ggttcccggg
     1561 tttctgccca cgggcctcgg cgccatcacc cgcggcgtct tccgcatgcc ccacttcaag
     1621 gagcgcgccg gccgctacgc ggacgtcatt cacgagggcg gcgcctgtgc caccagccag
     1681 ctcgtcctgc agggcggcat gcctcacgga ccgtcggcca ggctcgccaa ctacaccaac
     1741 aactccgtgc cccacgtgct ggacaaggac gagatccgct ggctggtgga ggagtacgcc
     1801 tactcggccc ggcagattgc ggatgccggc ctggacggcc tggagctgca cgccaaccac
     1861 gaggacctgc tggagctgtt tctttccccg gccaccaatc atcgggagga cgagtacggc
     1921 ggcgatctga atggacgtct gcgcttcatc aaggagatcc tggaggccat ccgggccgag
     1981 gtgggcaact ccatcaccgt gggcgtgcgc ctgaacatgg acgagttctt cgaaggcggc
     2041 tacgacctcg acgaagggct cgagatcgcc aaaggcctcg atgccaccgg cca
//
LOCUS       DBNHOX010000450         2404 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_999975, whole genome shotgun sequence.
ACCESSION   DBNHOX010000450 DBNHOX010000000
VERSION     DBNHOX010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_999975"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="AC16UZ_10025"
     CDS             <1..1246
                     /locus_tag="AC16UZ_10025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6247061.1"
                     /translation="VGVGEASASPAAFSMLSDYFSPRVRATVLAIYSSGVYIGAGIGV
                     FLGGIIVDSWNDAFPDPVLAPFGLKAWQAAFFAVGLPGLLMAIWVWTLREPQRGVSEG
                     LVVPTHPHPFRETARSLVTVLPPFTVVSLWVSGGARTVAVNLGLALLIAAVGYGANLA
                     TGTPNQWIALGIGVYAVVSWAQGLKLSDPVAFGMIFGSRAMVLLTLGFPCLAFVTYGV
                     GFWSPPYLLRAHGVNLAEAGTLLGLGSALGGWIGVTLGGIVADRLRARSVNGRIYIGV
                     ASPFLAAPAAIWFVLTDNVWVAYFCAFVFSMFSPMWLGAAASTVNDLVLPRMRAVASA
                     YYILMLTFIGLALGPYLIGQVSDIYLASGLDDGQALGRAILWGCGMLGVSLLFLAGAM
                     RYLGAEEANRLDRARALGEPMD"
     gene            1306..1857
                     /locus_tag="AC16UZ_10030"
     CDS             1306..1857
                     /locus_tag="AC16UZ_10030"
                     /inference="COORDINATES: protein motif:HMM:NF017999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6247062.1"
                     /translation="MEQFGEYRISAARAMVWEGLNDPEVLGRCIQGCLSMERTSDEQF
                     DASVKAKVGPVSATFQAQLMLSEIDAPSSYTLTANVKGGAAGFGKGTAKVSLLEDGAS
                     TLLRYQVSANVGGKLAQVGSRLIDAAARKMADDFFAEFGREVGGAEPEALAAVAEAPV
                     AGGRWKIWVAVAAIVVIILLLMV"
     gene            1897..2355
                     /locus_tag="AC16UZ_10035"
     CDS             1897..2355
                     /locus_tag="AC16UZ_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6247063.1"
                     /translation="MKVALNVNGTDRELEVPDNTLLVDLLREHLRLTGTHVGCDTSQC
                     GACTVHLNGRSVKSCTVLAAHAEGAEVVTIEGIGAPDDLHPMQEAFRDCHALQCGFCT
                     PGMIMSAIDLVNQSDDLDNQAVREGLEGNICRCTGYHNIVRAVLQAAEKM"
BASE COUNT          365 a          738 c          830 g          471 t
ORIGIN      
        1 ggtcggcgtg ggtgaggcga gcgcgtcgcc ggccgctttc tccatgctct ccgattactt
       61 ctcgccgcgc gtgcgggcca ccgttcttgc catctattcc agtggggtct acatcggcgc
      121 cggtatcggc gtcttcctcg gtgggatcat cgtcgattcc tggaatgacg cctttccgga
      181 tcccgtcctg gcgccgttcg gcctcaaagc ctggcaggcg gccttctttg cggtaggcct
      241 acccggcctg ctcatggcca tctgggtctg gacgctgcgc gagccgcagc gcggggtcag
      301 cgaggggctt gtggtgccga cgcacccgca tcctttccgc gaaacggccc ggtcgctcgt
      361 caccgtgctt cccccgttca ccgtggtctc gctatgggtc agcggcggcg cccggaccgt
      421 cgccgtcaat ctcggactcg ccctgctcat cgcggccgtc ggctacggtg cgaacctggc
      481 gacgggcacg ccgaatcaat ggatcgctct gggaatcggc gtctacgcgg tggtctcgtg
      541 ggcccagggc ctgaagcttt cggatcctgt ggcgttcggc atgatcttcg gctcccgtgc
      601 catggtgctg ctgaccctgg gcttcccctg cctggctttc gtgacctacg gcgtgggttt
      661 ctggagcccg ccctatctgc ttcgcgccca tggcgtcaat ctcgccgagg ccggcacgct
      721 gctgggcttg ggttccgccc tgggcggctg gataggcgtc acgctgggcg gcatcgtggc
      781 ggaccggctg cgggcgcgtt cggtgaacgg ccgcatatac attggcgtgg cttcgccctt
      841 tctcgccgca cccgcggcca tctggttcgt gctgaccgac aacgtctggg tggcctattt
      901 ctgcgcgttt gtgttcagca tgttctcgcc catgtggctt ggtgccgcag cgagcacggt
      961 gaatgatctt gtcttgccgc gaatgagggc ggtggcgtcc gcctactaca tcctgatgct
     1021 caccttcatc gggctcgccc tcgggcccta tctgatcggc caggtgagcg acatctatct
     1081 ggcgtcgggc cttgatgatg gccaggcgct gggcagggcg attctctggg gctgcggcat
     1141 gctgggcgtc agcctcctgt tcctcgccgg cgccatgagg tacctcggag ccgaggaggc
     1201 caaccggctc gaccgcgcgc gggccctggg agagcccatg gactgaacgc gttgccggga
     1261 taaggcaatc gtgcgaaatt aggcgctcga gggaggaggt tccgagtgga gcagttcggc
     1321 gaatatcgca tttccgccgc acgggcaatg gtatgggaag gcctgaacga ccccgaggtg
     1381 ctcgggcgct gcatccaggg ctgcctgtcc atggagcgga cgtcggatga gcagttcgat
     1441 gcctcggtca aggccaaggt gggtccggtc agcgctacct ttcaggcgca gctcatgctc
     1501 agcgagatcg acgcgccctc cagctacacg ttgacggcca acgtcaaggg aggtgccgcc
     1561 gggttcggga aagggacggc gaaggtcagc ctcttagaag acggtgccag cacgttgctc
     1621 cgctatcagg tgagcgccaa cgtcggtggc aagctcgctc aggtgggatc ccggctgatc
     1681 gacgcggcag cccgaaagat ggcagacgat ttctttgccg agttcggacg cgaggtgggc
     1741 ggtgcggagc ccgaggctct ggcggctgtg gcagaggcgc ctgttgccgg cgggcgctgg
     1801 aagatatggg tggccgttgc ggccattgtg gtgatcatac tgctgctcat ggtctgagcg
     1861 gccgtcgtcg tgaatcaaag agtctaggat tcgaatatga aagtcgcgct gaacgtcaat
     1921 ggtaccgacc gcgaactcga ggtgccggac aacacgctcc tggtggacct cctgcgggag
     1981 cacctcaggc tcacgggcac ccacgtcggc tgcgatacga gccagtgtgg cgcgtgcacc
     2041 gtgcatctca atggccgctc ggtcaagtcg tgcacggtgc tcgccgcaca cgccgaaggc
     2101 gccgaggtgg tgaccatcga gggaatcggc gcgccagacg acctgcaccc catgcaggag
     2161 gcgttccgcg actgccacgc gcttcagtgc gggttctgca cgccggggat gatcatgagc
     2221 gccatcgatc tggtgaatca gtcggacgac ctggataacc aggcggtccg ggaaggcctg
     2281 gaaggcaaca tctgccggtg cacgggttac cacaacatcg tgcgtgccgt gctgcaagca
     2341 gccgagaaga tgtgaggatt cgatatgagt gaagcagcgg caacggggat cggagcgtcg
     2401 gtca
//
LOCUS       DBNHOX010000451         2350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_614754, whole genome shotgun sequence.
ACCESSION   DBNHOX010000451 DBNHOX010000000
VERSION     DBNHOX010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_614754"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="AC16UZ_10040"
     CDS             <1..1120
                     /locus_tag="AC16UZ_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6247064.1"
                     /translation="DGDTISVYGEVIWDITDQVQLTGGLRWIEEKKDSFFFQPYVNSF
                     LAPGGGLPLFVPFDPDNPDTLVEADQKFDKTVPEVTLRWEPTDSLTFYVAYKEGFKGG
                     GFSNSGILSNAVFGSGADPAADFIFDEETVDGWEAGMKAAFLDGAMTVEVEVYDYDFQ
                     DLQIDFFNSPTFAFITTNAGASTTTGGELNVTWAPSGVAGLTLQGAVMYNEGEYDDFV
                     APCYAGQRPSAGCDIFNPGEVPKQQLGGSRRGLAPKWAGMLAADYEIGLAENLMLGLS
                     ANLQFKGSHQLSEFGIPAAVQDSYETLDAAIRLGSQDGRWQVAVIGKNLTDEYALLFS
                     SDTPGTGGGTGTDQGFLADQRGNPILPRTIAAELVVRF"
     gene            complement(1227..>2350)
                     /locus_tag="AC16UZ_10045"
     CDS             complement(1227..>2350)
                     /locus_tag="AC16UZ_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6247065.1"
                     /translation="HTGLWPHEPVSFAGKLVAVIGTGASGVQLIPEVAKAAEHLTVFQ
                     RTPNWCAPLHNASIDDEGQREIHRHYPDIFQLCSETHGAFMHKADPRNALEVSPEERE
                     AFYEQLYAEPGFGIWMGNFRDVMLDEQANETISAFVADKIRSRVDDPEVAEKLIPTNH
                     GFGTRRVPMETGYYEVYNQDNVTLVDVNETPIERITESGIRTSAADCEFDMIIYATGF
                     DAVTGALDRIDVRGRGGVSLKEKWADGPRTFLGLLVRGFPNLLTLVGAHNAASFCNIP
                     RCIEQNVEWVTDLVQHMQAHGYRRVEAAEAAEDAWTEHVYESAERMLFSKVDSWFMGI
                     NRNLGKEKRTFLLYAGGAPAYRERCEEVAASGYEGMELS"
BASE COUNT          404 a          802 c          724 g          420 t
ORIGIN      
        1 cgacggcgac accatctccg tctacggcga ggtcatctgg gacatcaccg atcaggtgca
       61 gctcaccggc ggcctgcgct ggatcgagga gaagaaggac tccttcttct tccagcccta
      121 cgtgaactcg ttcctggcgc ctggtggagg ccttcctctc ttcgtgcctt tcgaccctga
      181 caatccagac actctcgtcg aggcagatca gaagttcgac aaaactgtcc ccgaggtgac
      241 gttgcgatgg gagccgacgg acagcctcac cttctacgtg gcctacaagg aaggcttcaa
      301 gggcggcggc ttctccaaca gcggtattct gtccaacgcc gtcttcggca gcggcgccga
      361 tccggccgcc gacttcatct tcgacgagga aaccgtggac ggctgggagg ccggcatgaa
      421 ggccgccttc ctggacggcg ccatgaccgt ggaggtcgag gtctacgact acgacttcca
      481 ggacctgcag atcgacttct tcaactcgcc caccttcgcg ttcatcacca ccaatgcggg
      541 cgcctccacc accacgggcg gcgagctgaa cgtgacctgg gcgccgagcg gcgtggcggg
      601 cctcaccctg cagggagccg tcatgtacaa cgaaggcgag tacgacgact tcgtcgcccc
      661 ctgctacgcc ggtcagcgcc cctcggccgg gtgcgacatc ttcaatccgg gcgaggtgcc
      721 caagcagcag ctgggcggtt cccgccgcgg tctcgcgccc aagtgggcgg gcatgctggc
      781 ggccgactac gagatcgggc tggccgaaaa cctcatgctg ggcctatcgg ccaacctgca
      841 gttcaagggc agccaccagc tgagcgagtt cggcatcccg gccgcggttc aggactccta
      901 cgagaccctg gacgccgcca tccgcctggg cagccaggat ggacgctggc aggtagccgt
      961 gatcggcaaa aacctgacgg acgagtacgc gttgctgttc tccagcgaca cgcccggcac
     1021 cggtggcggc accggaacgg atcagggctt cctcgcagac cagcgcggca accccatcct
     1081 accgcgcacc atcgcggccg agctcgtggt cagattctga tctgatttcg accggacggc
     1141 ggcgcccgcc gccgtccggc tcgatctgaa aaccccttcc tctaaggcat cggcccgaca
     1201 agccgggagc aacctcggct cggctctcag ctgagctcca tgccttcata gccgctggcc
     1261 gccacttcct cgcagcgctc gcgatatgcc ggcgcgccgc ccgcgtacag cagaaacgtg
     1321 cgcttctcct tgccgagatt gcggttgatc cccatgaacc aggaatccac cttgctgaac
     1381 agcatgcgct cggcgctctc gtagacgtgc tcggtccagg cgtcttcggc cgcctcggcg
     1441 gcctccaccc ggcggtagcc gtgggcctgc atgtgctgca ccagatccgt cacccactcc
     1501 acgttctgct cgatgcagcg aggaatgttg cagaagctcg cggcgttgtg agcacccacg
     1561 agggtgagca gattgggaaa gccgcgcact aaaaggccca gaaacgttct cgggccgtcc
     1621 gcccactttt ccttgagcga caccccaccg cgacccctca cgtcgatccg gtccagcgcg
     1681 ccggtcaccg cgtcgaagcc ggtggcgtag atgatcatgt cgaactcgca atcggctgcg
     1741 ctggtgcgga tgccggactc ggtgatccgc tcgatgggcg tttcgttgac gtccaccagg
     1801 gtcacgttgt cctggttgta gacctcgtag tagccggtct ccatgggcac ccggcgggtg
     1861 ccgaaaccgt ggttggtggg gatgagcttc tccgccactt ccggatcgtc aacgcggctg
     1921 cggatcttgt cggccacgaa cgcgctgatg gtctcgttcg cctgctcgtc gagcatcacg
     1981 tcgcggaagt tgcccatcca gatcccgaat ccgggctccg cgtagagctg ctcgtaaaac
     2041 gcctcacgct cctcgggcga gacctccaac gcgttgcggg ggtccgcctt gtgcatgaag
     2101 gccccgtggg tctcgctgca aagctgaaag atgtccgggt agtgccgatg aatctcacgc
     2161 tggccctcat cgtcaatgga agcgttgtgc agcggcgcgc accagttcgg ggtgcgttga
     2221 aacacggtga gatgctcagc cgctttggcc acctccggaa tgagctgcac gccgctggcc
     2281 ccggtgccga tcacggcaac cagcttgccg gcgaagctca ccggctcgtg gggccacagc
     2341 cccgtgtgat
//
LOCUS       DBNHOX010000452         4014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_486663, whole genome shotgun sequence.
ACCESSION   DBNHOX010000452 DBNHOX010000000
VERSION     DBNHOX010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_486663"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1409
                     /locus_tag="AC16UZ_10050"
     CDS             252..1409
                     /locus_tag="AC16UZ_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247066.1"
                     /translation="MAISAVVDFLEATSSDDALKAELVKTLGVGDGDVSDAAELDENE
                     SAALLGQAGVDACALAAGKGFDFSVNELQAVVGAFQSFRSGEITDEELMSMLGLPSVP
                     SPSAQEAVMLSYRGVTHARATSGAGEGKRRLDVVRFVEATAKDDRLRSELQSILQAGD
                     GDISDFSELDEGELQALRSARGALVADFAAKHGYTFTIADLYSIIDAFQRMKGGELSD
                     TAFEKFVDASSQSADYLPFIDSIATMTYKGFTYDRVKPAVAQGNSLEVVRFLQKSHDD
                     ADLRGKLQEIIGGDGDVSSPEELDSSEAGNLIGARSARVVALATSEGFRFTISDLSAV
                     IGAFQLVEQGTLSLESCKRILGFADSAASEAFGNAGTTAVRIYRGVPVAAK"
     gene            complement(1448..1690)
                     /locus_tag="AC16UZ_10055"
     CDS             complement(1448..1690)
                     /locus_tag="AC16UZ_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIR6247067.1"
                     /translation="MCEACVEEMALKVQAAEETARSCPVDGAKMTKEIAHMIVIDRCP
                     TCQGVWLDGGELDRIRGGVEQEALMAMTGRFTYPLA"
     gene            complement(1865..3076)
                     /locus_tag="AC16UZ_10060"
     CDS             complement(1865..3076)
                     /locus_tag="AC16UZ_10060"
                     /inference="COORDINATES: protein motif:HMM:NF015406.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HIR6247068.1"
                     /translation="MSWPANRSAGLSRLEDWVPRAGRAYQARRNYDCGPGDRQNVSGL
                     SPYIRHRLVDEREVLEAVLAAHAHSAADKFVQEVFWRTYWKGWLEHHPQAWARYRTEL
                     EAVLETLSLDASTSERYGRAVAGETGMEPFDGWVEELTANGYLHNHARMWFASIWVHT
                     LGLPWVLGADFFMRHLLDGDPASNTLSWRWVVGLHTAGKTYLARPDNITRYTEGRFQN
                     ISGLVGAAHALQEAELARETLRLPGFCLPSGARLGLLLTDEDLSLPELPTNPVAIKGV
                     SLTRMRSPLGVSGAVRAFADDAIQDALGRASERYGCAAVGEIGAEDQEGVLAWVREQS
                     LDGVLVNYPTVGPARQFLEALAAPLGDAHVGSFPLVSEYDRLCWPHADRGYFKLRKRI
                     PELLGALDIAA"
     gene            complement(3080..3394)
                     /locus_tag="AC16UZ_10065"
     CDS             complement(3080..3394)
                     /locus_tag="AC16UZ_10065"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6247069.1"
                     /translation="MRSLNRIFVASILVLSLSAGQTAAAEAGEGAALASEKGCVACHG
                     KDGVAIAPIYPNLAGQWKRYLREQLHAYRSGRRQNNVMQGFAAGLSDDDIRRLADHYG
                     SR"
     gene            complement(3407..3730)
                     /locus_tag="AC16UZ_10070"
     CDS             complement(3407..3730)
                     /locus_tag="AC16UZ_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6247070.1"
                     /translation="MIEPSKSPVRFARGWLLAGVLLAGAALADPGRDLAWKYHCVTCH
                     GENGRSTQARYPNIAGQNAPYLEARLKYFRDGVEHNNRMNGQAAPLSDEDIAGLASYF
                     SRMPR"
BASE COUNT          771 a         1349 c         1189 g          705 t
ORIGIN      
        1 tggcgggtac ccgcgggtcg aggtggtctg gagccgctgc cgcgcaacaa tggatccgcg
       61 cgggggttgc cagacaattc ttatgcccgc cgcacgcttc cggagcgccg caagtgcccg
      121 cccgccctgg tttggtgcgc ccaggccggg aagatcgcca gatctcacgc attacgcagc
      181 ggtggtttct ggcccgaaag ttgctataac tccaataacg acgttgcgtt cagctcgcag
      241 aaggagagga aatggcgatc agtgccgtgg tggatttctt ggaagcaacg agttccgacg
      301 atgccctgaa agcagaactc gtaaagacgc tcggcgtggg cgacggtgac gtgagcgacg
      361 cggcggagct cgacgagaac gaatccgcag cattgctcgg ccaggccggc gtggatgcct
      421 gcgctctggc ggccggcaaa ggcttcgatt tctccgtcaa cgagcttcag gcggtggtgg
      481 gcgctttcca gagcttccgg tccggcgaga ttaccgacga agagctcatg agcatgcttg
      541 gcctgccgag cgtcccttcg ccaagcgctc aggaggccgt gatgctctcc taccgggggg
      601 tgacccacgc cagagcgaca tccggagccg gcgaaggcaa acgaaggctc gatgtcgtcc
      661 gcttcgtgga ggccaccgcc aaggacgacc gactgcgttc agaactccag tccattctgc
      721 aggctggcga cggtgacatc agcgacttct ccgagctcga cgaaggcgag ctgcaggcac
      781 tgcgcagcgc caggggcgcc ctggtcgcgg acttcgccgc aaagcacggc tacacgttca
      841 ccatcgcgga tctgtactcg atcatcgacg ccttccagcg gatgaaaggc ggcgagcttt
      901 ccgacaccgc ttttgaaaag ttcgtcgatg ccagcagcca gtccgccgac tatctgccct
      961 tcatcgactc catcgcgacc atgacctaca aagggttcac ctacgaccgg gtgaagcccg
     1021 ccgtcgccca gggcaactcc ctggaggtcg tgcgcttcct gcagaagtcc cacgatgacg
     1081 cagaccttcg tggcaagctt caggaaatca tcggcggcga cggcgacgtc agctcacctg
     1141 aggaactcga cagttccgag gcgggaaacc tgattggcgc gcgcagcgcc cgggtggtgg
     1201 cactagccac ctccgaagga tttcgcttca cgatctccga tctgagcgcc gtgatcggcg
     1261 cttttcagct cgtcgagcaa ggcacactgt cactggagag ctgcaagcgc attctagggt
     1321 tcgcggactc cgcagcgagc gaagcattcg gcaacgcggg gaccacggcc gttcgcattt
     1381 atcgcggcgt tccggttgcc gccaagtgag gctaccgagc gctcctcaaa cctgcacgcc
     1441 ggcgcctcta ggctagcggg taggtgaacc gccccgtcat ggccatcagc gcttcctgct
     1501 cgaccccgcc gcgaattcgg tcgagctcgc cgccgtcgag ccagactccc tggcaggtgg
     1561 ggcagcgatc aatcacgatc atgtgagcaa tctctttggt catcttcgcg ccatccacag
     1621 gacacgaccg ggctgtctcc tcagcagcct gaaccttcag cgccatctct tccacgcagg
     1681 cttcacacat ggggcggccg tcctgcttat tcttcgttct cgtaccgcac cgatcgcact
     1741 tctcgccgaa cagcgacata gcatttctcc tcctccgagg cccctatgat aatgccccat
     1801 ctgcgcccac cacaatgaac tcctgcccct gccgagcccc tgccggtctc gtccacggac
     1861 gatttcaggc ggcaatgtcg agagcgccaa ggagctcggg aatccgtttg cgcagcttga
     1921 aatagccgcg atccgcgtga ggccagcaca gccgatcgta ttctgaaacg agcgggaacg
     1981 atcccacgtg cgcatctccc aggggcgcgg ccagagcctc caggaactga cgcgcaggtc
     2041 cgaccgtcgg gtaattgacc aggaccccgt ccagagactg ctcccgcacc caggcgagga
     2101 cgccctcctg gtcctcagct ccaatctccc caacggccgc gcatccgtac cgctcactcg
     2161 cccggcccag ggcgtcctgg atcgcatcat cggcaaacgc ccgaaccgcc ccagatacgc
     2221 caagcggaga ccgcatgcgc gtcaggctca cacctttgat cgccacaggg ttcgtgggca
     2281 gctcaggcag cgatagatcc tcatccgtga gcagaagccc aaggcgagcg cccgacggca
     2341 ggcagaaccc gggcaggcgc agcgtctcgc gcgcaagctc ggcctcctgc agggcgtgcg
     2401 cggccccgac gaggccggag atgttctgaa agcgcccttc agtgtaacga gtaatgttgt
     2461 ccggccgcgc aaggtaggtt tttccggccg tgtgcaaccc tacgacccag cgccaggaca
     2521 aggtgttcga cgccggatcc ccgtcaagga ggtgacgcat gaaaaaatcc gcacccagca
     2581 cccagggcag tccaagcgtg tggacccaga tgctggcgaa ccacatgcgg gcgtggttgt
     2641 gcaggtaacc gttcgccgtg agctcttcga cccagccatc gaacggctcc atgcccgttt
     2701 cccccgccac ggctcgcccg tagcgctccg acgtcgacgc atccaacgaa agcgtctcca
     2761 gaacggcttc cagctcggtg cggtagcgcg cccaggcctg gggatgatgc tccagccagc
     2821 ctttccagta agttcgccag aacacctcct gaacgaactt gtccgccgcc gagtgggcgt
     2881 gcgcggcgag cacggcctcg agcacctcgc gctcatcgac gagtcggtgt ctgatgtaag
     2941 gcgacaggcc cgagacgttc tggcgatccc cggggccaca gtcgtagttg cgacgcgcct
     3001 gataggcccg gccggctcgc ggaacccagt cttcgagcct gctcagcccg gcagaccggt
     3061 tggccggcca gctcatcgtc tacctggaac catagtgatc ggccagcctc cggatgtcat
     3121 cgtcgctaag cccggcggca aaaccctgca tcacgttgtt ctgacgcctg ccggaccggt
     3181 aggcgtgcag ctgctcgcga aggtaacgtt tccactgtcc ggccaggttg ggatagatgg
     3241 gcgcgatggc tacgccgtct ttcccatgac aggcaacgca ccccttctcc gaggcgagcg
     3301 ccgctccctc acctgcttct gcggcggccg tttgcccggc ggaaagactt aaaaccaaaa
     3361 tcgacgccac gaatatcctg ttcaaacttc tcacttccat ttcccgtcac cgcggcatgc
     3421 ggctgaaata actggcaaga cccgcgatgt cctcgtcgga gagcggagcc gcctgaccgt
     3481 tcatgcgatt gttgtgctcg acgccatcgc gaaagtactt caaccgggct tccagatacg
     3541 gcgcgttctg cccggcaatg ttcgggtacc gggcctgcgt cgaccggccg ttctccccgt
     3601 gacaggtcac gcagtggtac ttccaggcga ggtcccggcc cggatccgcg agagcagcgc
     3661 cggcgagcag aacaccggcg agcagccacc cccgggcgaa gcgcaccggg cttttcgacg
     3721 gttcgatcat ctttttcctt tttgtctaac ttaatacacg actgataata gacttaatat
     3781 agacattaat catccattcg cccaattaaa ttagacaaat ttgacagata cgggtcagag
     3841 ggcgcgcaac ccggcaggca cttcagtcgg agccttcacg ccggattgac ccccggcgcg
     3901 ataacttgcg acgccgtaaa agtcaccagg agagcgccat cagcgcagac atcgccatca
     3961 tcggagccgg cctggccggc tgcacctgcg catctgccct ggccaggcac ggcc
//
LOCUS       DBNHOX010000453         3361 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_487023, whole genome shotgun sequence.
ACCESSION   DBNHOX010000453 DBNHOX010000000
VERSION     DBNHOX010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_487023"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="AC16UZ_10075"
     CDS             <1..934
                     /locus_tag="AC16UZ_10075"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA modification GTPase"
                     /protein_id="HIR6247071.1"
                     /translation="TAAARGAVRSLQGVFSERVRKIDEAVLGVRMYVEAAIDFPEEEV
                     DFLSEGDIAARITAILADVDDLIRDSRQGVLLRDGVTIALLGAPNVGKSSLLNALAGE
                     DRAIVTDIPGTTRDLVRADLELAGIPVTVVDTAGLRTTDDPVEREGVRRAEDQARSAD
                     LILVVTDVTDPNECSIELPEASQEIPVIQVHNKIDRTSAPPGPLTGEPLPVVRVSALT
                     GDGIDELRAQILAAVGYTEHAGTFTARQRHLLALQAAAAGLARAAGLAASADGGELVA
                     EELRGVHTELGTLLGGITADDLLGEIFSSFCIGK"
     gene            1008..2945
                     /gene="mnmG"
                     /locus_tag="AC16UZ_10080"
     CDS             1008..2945
                     /gene="mnmG"
                     /locus_tag="AC16UZ_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR6247072.1"
                     /translation="MQFPESFDVIVIGAGHAGAEAAAAAARVGARTLLLTQSIETIGQ
                     MSCNPAIGGIGKSHLVREIDALDGIMAKAADRAGIQFRILNSSKGPAVRATRAQADRA
                     LYRQAIRELLEAQENLYLFQQSVVDLIVEDGSVRGVVTQIGLEFRAPCVVLTTGTFLG
                     GRIHIGLDNHPGGRAGDAPSNALAERLRALPFRVDRLKTGTPPRIDARSVDFSVMAEQ
                     PGDEPIPCLSFLGSPDDHPRQVSCHVTHTNDRTHSLIRAALDRSPLYTGVIEGVGPRY
                     CPSIEDKVVRFSERTEHQIFVEPEGLDTHELYPNGISTSLPFDVQVALVRSIAGFERA
                     HITRPGYAIEYDFFDPRDLHYSLETKALEGLFFAGQINGTTGYEEAAAQGLLAGTNAA
                     LKVAGRAPWCPRRDEAYLGVLVDDLVNMGTKEPYRMFTSRAEFRLRLREDNADHRLTP
                     VGRDLGLVGEKRWDAYTTKRERVERVHGRMATRNVIAGSEVADRLASLTGERMTRDQT
                     LITYLKRPGVGIAALVGLLGDVLDLQDGDLDGQVLAQVEVEAKYEGYIARQEEEIARI
                     RRHEGLKLDEGLDFAGIEGLSAELKQKLTEARPSTLARAARIPGMTAAGLSVLLVHAR
                     RARATGAVAAPEDEPARGSGG"
     gene            2896..>3361
                     /locus_tag="AC16UZ_10085"
     CDS             2896..>3361
                     /locus_tag="AC16UZ_10085"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070043 - rRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR6247073.1"
                     /translation="MLSPPRKTSRRVVPVAEAIAEELRGGAAELGMTLSACQVEKLVA
                     YADLLRRWNDKFNLISRRDVPRLVSRHLLDSLSISPWLGGETTLDLGSGAGLPGIPLA
                     IAHPEREFLLADRSARQARFVEQAAMRLGLSNVSVFAGDAAALEQSFACVVAG"
BASE COUNT          567 a         1066 c         1133 g          595 t
ORIGIN      
        1 cacggcagcc gctcgcgggg ccgtgcgcag cctccagggc gttttctccg agagggtccg
       61 gaagatagat gaagcggtac tcggtgtgcg gatgtacgtg gaagcggcca tcgactttcc
      121 ggaggaagaa gtggattttc tcagcgaagg cgacattgct gcccggatca cggccatcct
      181 ggctgacgtc gacgatctga tccgggattc ccggcagggc gttctgcttc gggacggagt
      241 caccatagcg cttctcggcg cgcccaacgt gggtaagtcg agtctgctga acgcgctggc
      301 cggagaggac cgggccatcg tgaccgacat tcccgggacc acgcgcgatc tcgtgcgcgc
      361 cgacctcgag ctcgcaggca taccggtcac ggtggtggac acggcgggtt tgcgaaccac
      421 cgatgacccg gtggaacgcg aaggcgttcg gcgggcagag gatcaggcgc gctcggcaga
      481 tctgatcctg gtggtcaccg acgtcaccga cccgaacgaa tgttcgattg agctgcccga
      541 agcgtcgcag gagatccccg tcattcaggt gcacaacaag attgaccgga caagcgcgcc
      601 acctgggcca ttgacgggcg aacccctgcc cgtggtcagg gtttctgcgc tcaccggaga
      661 cggtatcgac gagcttcgcg cccagattct cgctgccgtc ggctacaccg agcacgcggg
      721 aaccttcact gcccggcaaa ggcacctgct ggcgctgcaa gctgctgctg cgggcctggc
      781 gcgagccgct ggtctggctg cttcggccga cgggggcgag ctcgtggcgg aagagcttcg
      841 cggcgtgcac accgaactcg gcactctgct gggcggcatt acggccgacg acctgctcgg
      901 cgaaattttc tcgagcttct gcatcggcaa gtagccccgg ccgcgccctt ccaatcggaa
      961 tccgagtact tatacttcgc gggcttgcaa cctggcggat attagctttg cagtttcccg
     1021 agtcgttcga cgtgatcgtc attggtgccg gccatgccgg cgccgaagct gccgctgcgg
     1081 cagcccgggt gggggcccgg actctgctgc tcacccagag catcgaaacc atcgggcaga
     1141 tgtcgtgcaa tccggccatt ggtggaatag gcaagtccca cctcgtccgg gagattgacg
     1201 cgctggacgg gatcatggcc aaagccgccg atcgcgccgg catacagttc cgaatcctca
     1261 actccagcaa ggggccagcc gtcagggcca cccgcgccca ggcggacagg gccctttacc
     1321 gacaagccat ccgggagctg ttagaggccc aggagaacct ttacctcttc cagcagtcgg
     1381 tggtggatct gattgtcgaa gacggctcgg tccgtggtgt ggtcacccag atcggcctgg
     1441 aattccgggc gccctgtgtt gtgctgacga cggggacgtt tctcggcggc cgtattcaca
     1501 tcggcctgga caatcacccc ggcggccgag cgggagatgc accgtccaac gcgctggcgg
     1561 agcgtctgcg cgcgctgccg ttccgcgtgg accggctgaa gacgggtacc cccccgcgaa
     1621 tcgacgcccg ctccgtggat ttctcggtga tggccgagca gcctggggat gagccgatcc
     1681 cctgcctgtc ttttctcggc tcccccgacg atcacccccg tcaggtcagt tgccacgtca
     1741 cccataccaa cgatcggacc cactccctta ttcgcgcggc gctggatcgc tcacctctct
     1801 acaccggggt catcgaaggc gttgggccgc gatactgtcc gagtatcgag gacaaggtcg
     1861 tgcggttctc ggagcgaact gaacatcaga tattcgtcga gccggaaggg ctggacactc
     1921 acgagctcta ccccaacggg atctccacga gcctgccgtt cgatgtgcag gtcgccctgg
     1981 tgcgcagcat cgccggattc gagcgggccc acatcacgcg gccgggctat gccatcgagt
     2041 acgacttttt cgacccccgg gacctgcatt actcgctgga gacgaaggcg ctggaagggc
     2101 tgtttttcgc cggtcagatc aacggcacca ccggatacga ggaggcggcg gctcagggcc
     2161 tgctcgccgg aacgaacgcg gcgctcaagg tggccggccg ggcgccctgg tgtccccgcc
     2221 gcgacgaggc ctatctgggc gttctggtgg atgatctggt gaacatgggc accaaggagc
     2281 cctatcggat gttcaccagc cgggcggagt tccggctgag gcttcgcgaa gacaacgcgg
     2341 atcatcgcct caccccggta ggccgggatc tggggctcgt gggcgaaaag cgctgggatg
     2401 cgtacaccac gaagcgtgag cgggtcgagc gcgtccacgg gcggatggcg acccgcaacg
     2461 taatcgcggg gtcggaggtg gctgaccggc tcgccagcct caccggtgag cggatgactc
     2521 gagatcaaac gctcatcacc tatctcaagc gtccgggcgt gggcatcgcc gcgttggtcg
     2581 gtctccttgg ggatgttctg gacctgcagg atggggacct cgacggccag gtgctggcgc
     2641 aggtggaggt ggaagctaag tacgagggct acattgcgcg ccaggaagaa gagattgcca
     2701 gaatccgtcg gcacgagggc ctcaagctcg acgaaggcct ggacttcgcc ggtatcgaag
     2761 ggctttccgc agagctcaag cagaagctga ccgaggctcg accctcgacg cttgctcgcg
     2821 ctgcgcgtat ccccggaatg acggcggccg gcctgtccgt tctgctggtg cacgcccgaa
     2881 gagcccgggc tacaggtgct gtcgccgccc cggaagacga gccggcgcgt ggttccggtg
     2941 gctgaggcga tcgccgagga gctgcggggg ggcgccgccg agctgggaat gaccctatcg
     3001 gcctgccagg tggaaaagct cgttgcctac gccgatctcc tgcgccgatg gaatgacaag
     3061 ttcaacctga tctcgcggcg ggatgttcct cggttggtat cccgccacct cctcgacagt
     3121 ctgagcatct cgccatggct gggcggcgag actactctgg acctcggctc gggggccggc
     3181 ctgcctggaa tccctctggc catcgcccat ccggaacgcg aattcctgct ggctgaccgg
     3241 tcggcgcgcc aggcgcggtt tgtcgagcag gcggcgatgc gtctaggcct ttcgaacgtg
     3301 agcgtgtttg ccggggacgc cgccgccctg gagcaatcgt tcgcttgtgt ggttgccggg
     3361 c
//
LOCUS       DBNHOX010000454         2054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1643156, whole genome shotgun sequence.
ACCESSION   DBNHOX010000454 DBNHOX010000000
VERSION     DBNHOX010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1643156"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="AC16UZ_10090"
     CDS             complement(<1..339)
                     /locus_tag="AC16UZ_10090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014850970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HIR6247074.1"
                     /translation="MPRFSILDLAPITIGGSARQALHNARDLARHGEAWGFERFWLAE
                     HHNMPGIASSATAVAIGFVAEGTSTIRVGAGGIMLPNHAPLVIAEQFGTLAALYPGRI
                     DLGLGRAPGTD"
     gene            complement(355..747)
                     /locus_tag="AC16UZ_10095"
     CDS             complement(355..747)
                     /locus_tag="AC16UZ_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIR6247075.1"
                     /translation="MGDYVYPGILSSLALLVYYFTLFKAGMARGKYNIPAPSHEGPEE
                     YVRRVRVHQNTMEHLVLFLPGLWLFSYAVDPIWAAGIGVLWPVGRLWYAFAYYEAAEK
                     RGPGLYLSMPPIYIFVIGSLIGFMLELF"
     gene            complement(802..>2054)
                     /locus_tag="AC16UZ_10100"
     CDS             complement(802..>2054)
                     /locus_tag="AC16UZ_10100"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6247076.1"
                     /translation="GGRYTDSAFAAAEWDGMWSRVWLLLGRAREMPNPGDYQVEDVGP
                     ESFIMVRQDDGSIRAFYNVCQHRGSRLIFNAAGSVERISCPYHSWTYGRDGALLVVQD
                     PEDFSSGNPCERLSLVEVRCETFAGWVWVNMDPDCVSLREYLGPVWEDWQTWQPEDWR
                     RSLAISAWVPCNWKVIQDNFCESYHLLTVHPQLTANIEEGVPWTRFDMANEGHNRMIM
                     QAGMPSHRQAQGPKLVEPLLSQMAMWELDPDDFAGREHDTRAAIQQQMRDLGAPRGYA
                     HYDRLRDEQLTDPHHYNIFPNCSVTFGADSVLLQRMRPHPTDPQQCRFDHWTYVAPAA
                     IEHGVFRNSDGRVEFTGDAEIKEVEYGKESMGPIADQDIGITTGQQLGLRSRGYRGAN
                     LADQESRIARFHGVIDEYIAGERP"
BASE COUNT          356 a          673 c          676 g          349 t
ORIGIN      
        1 gtccgtgcct ggcgcccggc cgaggcccag gtcgattcgg cccgggtaca gggcggccag
       61 ggtgccgaac tgttcggcga tgaccagcgg cgcgtggttc ggcagcatga tgccgccggc
      121 tcccacgcgg atcgtggacg tgccttcggc cacgaaaccg atggcgacgg cggtggccga
      181 gctggcaatg ccgggcatgt tgtggtgctc ggccagccag aaccgctcga atccccaggc
      241 ttccccatga cgcgccaggt cgcgcgcgtt gtgcagcgcc tggcgtgcgg atccgccaat
      301 ggtgatgggg gccaggtcca gaatactgaa tcggggcatg ctcgctcccg cggtctaaaa
      361 cagctccagc ataaacccga tgagcgatcc gatcacgaat atgtagatgg ggggcatgct
      421 gagataaagc ccgggcccac gcttctcggc cgcctcgtag taggcaaacg cataccagag
      481 ccggccgacg ggccaaagaa ccccgatccc cgccgcccag atggggtcca cggcgtagga
      541 gaacagccac agcccgggca gaaacagcac caggtgctcc atggtgttct gatgcacgcg
      601 aaccctgcgg acgtactcct cagggccctc atgagacggc gccggtatgt tgtatttgcc
      661 ccgggccatg ccggccttga acagggtgaa gtagtaaacg agcagcgcca ggctgctcag
      721 aatgcccggg taaacgtagt cacccatgtc gctctcttcc gctcaccgaa gccggattgg
      781 cagccgtttc ccgatcgccg ctcaaggccg ctcgccggcg atgtactcgt cgatcacccc
      841 atggaaccgt gcgatgcgcg actcctggtc tgccagattg gcgccccggt aaccgcgcga
      901 gcgcagcccg agctgttgcc cggtggtaat gccgatgtcc tgatcggcga tgggacccat
      961 ggattccttc ccgtactcaa cttcctttat ctcggcgtcg cccgtgaatt cgacccggcc
     1021 gtctgagttg cggaacaccc cgtgctcgat ggcggctggt gccacgtagg tccagtgatc
     1081 gaaacggcac tgctgcggat cggtgggatg aggccgcatc cgctgcagca gcacagagtc
     1141 ggcaccgaag gtcaccgagc agttggggaa gatgttgtag tggtgcggat cggtgagctg
     1201 ctcgtcgcgc aggcggtcgt agtgagcata gccccgcgga gcgccgagat cgcgcatctg
     1261 ctgctggatc gccgcccggg tatcatgctc ccggccggcg aagtcgtcgg ggtcgagctc
     1321 ccacatggcc atctgggaga gcagcggttc cacgagcttt ggcccctggg cctggcgatg
     1381 ggacggcatg cccgcctgca tgatcatgcg gttgtggcct tcgttggcca tgtcgaagcg
     1441 cgtccacggc acgccttcct cgatgttcgc cgtgagctgc gggtgcacgg tcagcaggtg
     1501 gtaggactcg cagaagttgt cctgtatgac cttccagttg cagggtaccc aagcggagat
     1561 ggccagggac cggcgccagt cctcgggttg ccacgtctgc cagtcttccc acacgggacc
     1621 gaggtattcg cgcagcgaga cgcagtcggg gtccatgttg acccacaccc agccggcgaa
     1681 ggtctcgcag cgcacctcca ccaggctcag ccgctcgcag ggattcccgg agctgaaatc
     1741 ctcagggtcc tgcacgacca gcagcgcgcc gtcacggccg taggtccacg agtggtaagg
     1801 gcaggagatg cgctccacgg aaccggcggc attgaagatc agccgcgagc cccggtgctg
     1861 gcagacgttg tagaacgcgc ggatggaccc gtcgtcctgg cgcaccatga tgaaggactc
     1921 ggggcccacg tcctccacct ggtagtcgcc ggggttcggc atctcccggg cccgacccag
     1981 cagcagccag accctgctcc acatgccgtc ccactcggcc gccgcgaacg cgctgtcggt
     2041 atatcggcct ccct
//
LOCUS       DBNHOX010000455         1521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_936441, whole genome shotgun sequence.
ACCESSION   DBNHOX010000455 DBNHOX010000000
VERSION     DBNHOX010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_936441"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1395
                     /gene="hmgA"
                     /locus_tag="AC16UZ_10105"
     CDS             100..1395
                     /gene="hmgA"
                     /locus_tag="AC16UZ_10105"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003152943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HIR6247077.1"
                     /translation="MTGVLEYLAGFGNEHQTEALPGALPVGRFSPQRPALGLYAEQLS
                     STAFTVPRALNRRTWFYRIRPSVMHGDFRPMQTGLLRTSPVDQGAPLPDQMRWDPLEV
                     PEEPADFLDGLITMAVNGNAATQAGVGVHVYLATTSMGRRYLYDADGELLIVPQLGSL
                     LVRTECGALDVSPGEICVVPRGLKFAVDVPNPPVRGYVCENYGWPFLLPERGPVGANG
                     YANDRDFLYPTARFEDVDGHHELVCKLSGNLFVAEMDHSPLDVVAWTGNSAPYKYDLG
                     RFNVMGSVSFDHPDPSIYTVLTSPSETAGMANADFVIFPPRWIVAEDTFRPPWYHRNV
                     MSEFMGLVRGLYDAKETGFVPGGCSLHNSMAAHGPDVEAFEKASSADLKPQKLADTLA
                     FMFESRYPYTATAFAMESPALQSDYRACWAALPRMFEPQ"
BASE COUNT          244 a          492 c          497 g          288 t
ORIGIN      
        1 cagcaagacc ccgtcgttca aggccacacc ggtctgcata gaacggtctt tgactacgcc
       61 gctgcgggtg gagcccagtc gcggcgagaa cgccccctag tgacgggcgt cctcgagtat
      121 ctagcggggt tcggcaacga gcatcagacc gaggcgcttc ccggtgcgct ccccgtcggc
      181 cgcttcagtc cccagcggcc ggcgctcggc ctgtacgcgg agcagctgag ctcgacggcg
      241 ttcacggtgc cccgggccct caatcgccgc acgtggttct atcgaatccg gccatcggta
      301 atgcacggcg acttccgccc gatgcaaacc gggcttctgc gcacttcgcc cgttgaccag
      361 ggcgcgccgc tccccgatca gatgcgctgg gatccgctag aggtgccgga ggagccggcc
      421 gacttcctgg acggtctgat caccatggcg gtgaacggaa acgcggccac tcaggccggt
      481 gtgggcgtgc acgtctatct tgcgactacg tccatgggtc gtcgctacct ctacgatgcc
      541 gacggtgagt tgctgatcgt tccccagctg ggatcgcttt tggttcgcac ggagtgcggt
      601 gccctggatg tctccccggg cgagatctgc gtcgtcccgc gcggcttgaa gttcgccgtc
      661 gatgtgccga acccacccgt tcgcggctac gtctgcgaga actacggctg gccgtttctg
      721 ctccctgagc gcggtccggt gggcgcgaac ggatatgcca acgaccggga ctttctctac
      781 cccacggcac ggtttgagga cgtggacggt caccacgagc tcgtgtgcaa gttatcggga
      841 aatctgttcg tcgcggagat ggatcattcg cctctggacg tcgtggcctg gacaggcaac
      901 tcggcgccgt acaagtacga tctgggccga ttcaacgtca tgggctcggt gagcttcgat
      961 catcccgatc cgtccatcta cacggtgctc acctcgccct ccgagaccgc gggcatggcg
     1021 aacgcggact tcgtgatctt tccgccgcgc tggatcgtcg ccgaagacac ctttcgtccg
     1081 ccctggtacc accgcaacgt catgagcgag ttcatggggc tggtgcgggg tttgtacgac
     1141 gccaaagaga ccggcttcgt gcccggcggg tgcagcctgc acaacagcat ggcggctcac
     1201 ggtcccgatg tggaggcctt cgagaaggcg agctcggcgg atctgaagcc gcagaagtta
     1261 gccgacacgc tggcgttcat gttcgagtcc cgctatccct acacggctac cgcgttcgcc
     1321 atggaaagcc cggccctgca gagcgattac cgagcctgct gggcggcgct gcctcggatg
     1381 ttcgagccgc agtgatgcgc acggggatgg cggccggccc acgggcgggg tgagatgccg
     1441 atggtgccga cggagacgga aattcgtgaa ttcgacctgc gcaacccgcc ggccgggttc
     1501 gtagaggatc cctttttctg g
//
LOCUS       DBNHOX010000456         7858 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_550430, whole genome shotgun sequence.
ACCESSION   DBNHOX010000456 DBNHOX010000000
VERSION     DBNHOX010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7858)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_550430"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="AC16UZ_10110"
     CDS             complement(<1..1401)
                     /locus_tag="AC16UZ_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247078.1"
                     /translation="MRTRIALLSIAGLMASFAQAGPVDPSAITTFEAGQPARAAEVNA
                     NIDALITAIDDNASQLASSGQDVAIEVDCGDVREALQQAIDGSDPTKPLRINVLGTAT
                     CGPIAVRRDNVSVKGGNDTDTASRTIGTDGDGNAPVWVEGARHVTLSNLTIDAADGGT
                     SEVGVAVAKGALVNLDFVSVQNSTAEAVTVFVNSTAVLSGTNTFAGGSGRGLNIGGNA
                     TVFAENGETTISSTGGEPVLEMDANAVFGGGAAVNLSSTGVPANGTSINVAGNSDLGL
                     ENGSIELSGELEVRRSLVLIEPSGDGFTGPVFTLTAAAVDLLPGSRVVLASEDGASIE
                     STGSWFVNNDSSVVAVGAGTTLGGGSVFAQFGGTFGAGDGTDLNGMSGITARWNGQLI
                     LGGGMPTLTLLNDAIRVFDGGSLFSFDPALQTTDQVNCSRPPIFPAVTTTLSGTGSGT
                     VDGSVGPETVTVDNVTV"
     gene            complement(1503..2777)
                     /locus_tag="AC16UZ_10115"
     CDS             complement(1503..2777)
                     /locus_tag="AC16UZ_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247079.1"
                     /translation="MKPRLRLLLAACCVALLAGCDNDDDDDIVAPPAPEPVQTEPVNG
                     SGVKGPLAGAVVELYAIDLAQANIQGELIDDGSTGPDAAIQDLAVPADTTGKVLLLFV
                     VDDDTTDLTTGAAPLFDSLTTVVEAQRLIDGESVYASPLTTMAVDLAVAKGDSGSPYA
                     GNGDGTLDEAEFAAALDVAQGQVKSTLGFGMDSTIDIFTVPPLLTNDTDTADEQGDVA
                     AYRQAIEAVTAISAQIAADVEAAGGTASAQDAFNALTEDLTDGDLDGESEAGPIETVA
                     SLAEGELETIVTQDVTTLTIPGTETPIGEIESVLAAETADTGAETDTTDLESGAVSVD
                     PEPAETETDIDGDGVSDSADAFPSDPTETVDTDGDEVGDNSDAFPEDPTETADGDGDG
                     VGDNADQFPDDPTRTAPGGVWDESNWDEVDWQ"
     gene            2953..4230
                     /locus_tag="AC16UZ_10120"
     CDS             2953..4230
                     /locus_tag="AC16UZ_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6247080.1"
                     /translation="MSEATESIETCPIHAIRPMLPENLAYPVAMNRRLRKDAPVYQDP
                     DSGIWFESRYEDVINMSMDHKTFSSVMPRLSTGRADSAVDPEVAAAMAEGYPIPDTML
                     TQDPPLQRRYRKFVDGAFSPRSLKALEPFIEQTSNELIDAFIDQGHCEFLAEFGVQLP
                     LRVIASQIGCPMEDLPLLRKWTEAFIANLSQQLDQPGRVQAAKDMVECQHYFVERIEE
                     RRRAPTDDILSKLVNARFDDDKPLDNPECLSMLQQILVAGNETTSSSLTEGMWLLIRN
                     PDQYEMIRRDPSPEMISKLVEEVLRISSPSANMFRRTTRDVEVSGVTIPANSICFARF
                     AAANQDDEQFPDPLKFDITRPNLKEHVAFGKGVHHCLGAALSRREMNGGFKVIFERME
                     NFRLTPDTPEPVFMPNALLHGLARLDLSFDKIR"
     gene            4243..5874
                     /locus_tag="AC16UZ_10125"
     CDS             4243..5874
                     /locus_tag="AC16UZ_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6247081.1"
                     /translation="MGETVAEETVAADADAVVIGAGFAGLYMLHKLRQCGFSVRGFEA
                     GDDVGGTWYWNRYPGARCDVESLDYQFAFSDELQRGWTWSERYPTQAEILRYLGYVAD
                     TLDLRRDIRFETRVSAARFDEAHNLWRVTTDRGEELSARYVIAAVGCLSVAQIPDIPG
                     LQDFEGETYHTGNWPHQPVDFTGRTVGIIGTGSSGVQAIPVIAAQAKALVVFQRSPNF
                     SVPARNAPLPADRAERYKDEFLAAREAACRTDFGNTTWRSLPSALEADPDERAAEYER
                     RWQEGGAGFMVTYSDLMSDRAANDTAAEFLRGKIRAIVRDPETAELLSPRDHPIGAKR
                     ICVDTDYYETFNRENVKLVDVRSHPIERITASGISTTAGDYDLDCLIFATGFDAMTGP
                     LTSMDIRGRDGVSLRAAWQDGPRCYLGLGVAGFPNLFTITGPGSPSVLSNMVLSIEQH
                     VEWIADCLVKLRKDGAEAIEALPDAQDGWIEHVNEAADATLFPQGNSWYIGANVPGKP
                     RVFMPYTGGVRAYREKCEEVVGNSYEGFDIRGGAS"
     gene            5893..7137
                     /locus_tag="AC16UZ_10130"
     CDS             5893..7137
                     /locus_tag="AC16UZ_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HIR6247082.1"
                     /translation="MTSAARIAGLPSYFIVFVASMVAIGPFAIDTYLPAMPAMATNLG
                     VEIVSVNATLSTYLFGFAFGQMFGGPISDQIGRRRIGLLGLVVFSAASVLIALAESIE
                     VILWLRALQAVGGGFATVICMAMVRDAYEPMEAAKRFPVVMLVMLGAPLAAPAIGAAL
                     LSFGWPSIFIFLALYGLAVLVAFLPVPETASMRTGKLELGRILPQYAEVITRRVEGRL
                     VPARYILTQGLLMSGTFVFITNSSFIYLEYFGIAEEWFVVYFGVNILMMMVFTLITTR
                     LIHRVAPDRLWRAARGLQFLAVVMLALGSWLFDLNIWTLTGLLSVVIGANGMINPSAS
                     GLYLSYFDRLSGSAVSLMNVAVFLFGSVLGVVTGVFFDGTLKPIVFTMAVAITVGNLN
                     ALGIPPPKNLAPDASGESASAG"
     gene            complement(7185..>7858)
                     /locus_tag="AC16UZ_10135"
     CDS             complement(7185..>7858)
                     /locus_tag="AC16UZ_10135"
                     /inference="COORDINATES: protein motif:HMM:NF016071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HIR6247083.1"
                     /translation="ANQLYWSSGETFRTAAMGVQDAASDLGFSTADVEAAFSAVDVNL
                     GDVCPAEQVAELGNGASSGEFGASAGEWRCFRLSVTAGGTDASFVLAKTAKGRGGDAD
                     LYVQHAALPDTVRYDCRSVSSTSSESCAIATPLEGDWYVGVYAYSSYPGVTLTGSFTD
                     PSGSGGGGGDGGGSGSTDADSDGYTSDVDCDDNNPDVHPDHNDTKGRWGRDGVDNDCN
                     GTIDG"
BASE COUNT         1245 a         2620 c         2532 g         1461 t
ORIGIN      
        1 aaccgtgaca ttatcgacag taacagtctc ggggccaaca gagccatcta ctgtgcctga
       61 gcccgtgcct gaaagagtcg tcgtgactgc aggaaaaatt ggtggcctcg agcagttgac
      121 ctggtcggtc gtctgcaggg cggggtcaaa cgaaaataga ctgccgccat cgaatactct
      181 gatggcgtcg ttgagcagcg tcagcgtcgg catgccgccg ccgagaatta gctgaccgtt
      241 ccaacgagcc gtgatacccg acatgccgtt cagatcggtc ccgtctccgg caccgaacgt
      301 cccgccaaac tgggcgaata ctgatccgcc ccccagggtc gtgccagccc caacggccac
      361 cacgctcgag tcgttgttca cgaaccagct gccggtgctc tcgatgctgg ctccgtcctc
      421 agatgcgagc acaacgcgtg atcctgggag taggtccaca gcagccgcgg tcagggtaaa
      481 aacgggcccg gtgaagccgt cgccgctggg ctcgatcagc acgagggacc tacggacctc
      541 cagttcacca ctcagctcga tggagccgtt ctcgaggccc aggtcgctat tgccggccac
      601 gttgatgctg gtgccgtttg caggcacccc cgtgctgctc aggtttactg cagctccccc
      661 accgaacacc gcattggcgt ccatctccag gacgggctct ccgccggtgc tcgagatcgt
      721 cgtttcgcca ttttcagcga acacggtggc gtttccgccg atgttgaggc cgcggcctga
      781 gccgccggca aacgtgttgg ttccgctaag cacggcggtg ctgtttacga acacggtgac
      841 tgcctcagcg gtggagttct gcacgctgac gaagtccagg ttgaccagcg cgcctttggc
      901 caccgcgact ccgacctccg aggtgccacc gtcagcggcg tcaatggtca ggttgctcag
      961 cgtcacgtgt cgggcgccct ccacccatac cggagcgttg ccgtctccat cggtcccaat
     1021 cgttcttgag gccgtatccg tatcgttccc gccttttacg ctgacgttgt cccgccttac
     1081 ggcgatgggg ccgcacgtag cagttccgag cacgttgatc ctgagcggct tggtgggatc
     1141 ggagccgtcg atggcctgct gcagagcctc ccgcacatcg ccgcaatcca cctcgatggc
     1201 cacgtcctga ccggagctcg cgagctggga agcgttatcg tcgatggccg tgatcagcgc
     1261 atcaatgttg gcgttcacct cggcggctct ggcgggttgg ccggcctcaa aggtcgtgat
     1321 ggcggacgga tccaccgggc ccgcctgggc gaaagaggcc atcaggccgg caatggaaag
     1381 cagtgcgatg cgcgttctca tggtggactt gatcctcgtt tgtttgttca tctcttgact
     1441 cttggttcgc atgagggccg gtctgcgagc ggcccgccgg attgattgat tcgtgtaacc
     1501 gctcattgcc agtccacctc gtcccagttg ctctcatccc acacgccgcc gggtgcggtg
     1561 cgggtggggt cgtcggggaa ctggtcggcg ttgtcgccca cgccgtcgcc gtccccgtcg
     1621 gcggtctcgg tgggatcctc ggggaaggcg tcgctgttgt cgccgacttc atcgccgtcc
     1681 gtgtccacgg tctccgtggg atcgctcgga aacgcgtctg cgctgtcgct cacgccgtca
     1741 ccgtcgatgt cggtttcggt ctcggcgggt tccggatcca ccgagacggc cccgctctcc
     1801 aggtccgtgg tgtcggtctc cgcgcccgtg tccgcggttt cggcggcaag cacgctctcg
     1861 atctcgccga tgggcgtctc cgtgccggga atcgtcaggg tggtcacgtc ctgagtgacg
     1921 atggtctcaa gctcgccctc ggcgagggaa gcgaccgtct cgatgggtcc ggcctcgctc
     1981 tcgccgtcaa ggtcgccgtc cgtgaggtct tccgtgagcg cgttgaaggc gtcctgggcg
     2041 ctggccgtgc cgccggcagc ttccacgtct gcggcgatct gggcggagat ggcggtcacc
     2101 gcctcgatgg cctgacgata agcggcaacg tccccctgct catcggcggt gtccgtgtcg
     2161 ttggtcagta gcggcggcac cgtgaagatg tcgatggtgc tatccatgcc gaagccgagg
     2221 gtggacttca cctggccctg ggccacatcc agggcggcgg cgaactccgc ttcgtccagc
     2281 gtgccgtcgc cgttgccggc gtagggcgag cctgagtcgc ctttggccac ggccagatcc
     2341 acggccatgg tggtcagcgg ggaggcgtaa acgctttcgc cgtcgatgag ccgctgggct
     2401 tcgaccaccg tggtcaggga gtcgaagagc ggcgcggcgc cagtggtgag gtccgtggtg
     2461 tcgtcgtcga cgacgaatag gagcaggact ttgcccgtgg tgtccgcagg gaccgccagg
     2521 tcctggatgg cagcgtcggg acccgtggag ccgtcgtcga tcagctcgcc ctggatgttc
     2581 gcctgggcaa ggtcgatggc gtagagctcc accaccgcgc cggccagagg ccccttcacg
     2641 ccggaaccgt tgacgggttc ggtttgaacg ggctctggtg ctgggggcgc gacgatatcg
     2701 tcatcgtcgt cgttgtcaca tccggcgagc agcgcaacgc aacaggccgc cagcagtaat
     2761 cggagtcttg gcttcaagcg atctccccct atttgccagt tattagtcaa tcttgttgat
     2821 cagaaacttt ttttacctca tttagatcct gcgggcaata cgctggcaac gaaaggcaag
     2881 gtgcgctagt ttccccgctc cccgacgcag cgctgagggg cgcatcggcc tgccattcag
     2941 agacaaagac ccatgagcga agcaacagaa tccatcgaaa cctgcccgat ccacgccatc
     3001 cggcccatgc tgccggagaa cctggcctac ccggtggcca tgaaccggcg gctgcgcaag
     3061 gacgcacccg tctatcagga cccggacagc ggcatctggt tcgaatcccg atacgaggac
     3121 gtgatcaaca tgtccatgga tcacaagacg ttctccagcg tcatgccgcg cctgtcgacc
     3181 ggtcgggcgg acagcgccgt cgacccggag gtggcggcgg cgatggccga aggctacccg
     3241 atcccggaca ccatgctgac ccaggatccg cccctgcagc gccgctaccg caagttcgtg
     3301 gacggcgcgt tctcgccccg gagcctcaag gccctggagc ccttcatcga gcagacgtcc
     3361 aacgagctca tcgacgcctt catcgaccag ggccactgcg agttcctggc cgagttcggc
     3421 gtgcagctgc cgctgcgggt catcgcctcc cagatcggct gccccatgga ggacctgccg
     3481 ctgctcagga agtggacgga agccttcatc gcgaatctct cccagcagct cgaccagccg
     3541 ggccgggtgc aggcggcgaa ggacatggtg gagtgccagc actacttcgt ggagcgcatc
     3601 gaggagcgcc gccgcgcgcc gacggacgac atcctctcca agctcgtcaa cgcccgcttc
     3661 gatgacgaca agccgctgga taacccggaa tgcctgtcga tgctgcagca gattctggtg
     3721 gccggcaacg agaccacctc gtcgtccctc accgaaggca tgtggctgct gatccgcaat
     3781 cccgaccagt acgagatgat ccgccgggac ccgtcgccgg agatgatcag caagctggtg
     3841 gaggaggtgc tgcgcatctc cagcccgtcg gccaacatgt tccgccgcac cacgcgggac
     3901 gtggaagtga gcggcgtgac catccccgcc aactccatct gcttcgcccg ctttgcggcg
     3961 gccaaccagg acgacgagca gtttccggat ccgctgaagt tcgacatcac ccggcccaac
     4021 ctgaaggagc acgtagcgtt cggcaagggc gtgcaccact gtctcggcgc ggcgctgtcg
     4081 cgccgtgaga tgaacggtgg gttcaaggtc atcttcgagc gcatggagaa cttccgcctg
     4141 acgccggaca cgccggagcc ggtgttcatg ccgaacgcct tgctgcacgg gctggcgcgg
     4201 ctggatcttt ccttcgacaa gatccgttga tcgcaagcgg gcgtggggga gaccgttgca
     4261 gaggagaccg tcgctgctga cgccgatgcc gtcgtcatcg gtgcgggctt cgccggcctc
     4321 tacatgctgc acaagctgcg gcagtgcggc ttctcggtac gcggcttcga ggcaggcgac
     4381 gacgtgggcg gcacctggta ctggaaccgc tacccgggcg cgcgctgcga cgtggagagc
     4441 ctggactacc agttcgcctt ctccgacgag ctgcagcgcg gctggacgtg gtccgagcgc
     4501 taccccaccc aggccgagat cctgcgctac ctcggctacg tggcggacac cctcgatctg
     4561 cgtcgggata tccggttcga gacccgggtg agcgccgcgc gcttcgacga ggcgcacaac
     4621 ctttggcggg tgaccacgga ccggggcgag gagctcagcg cccgctacgt gatcgccgcc
     4681 gtgggctgcc tctccgtggc ccagatcccc gacatccccg ggctgcagga cttcgagggc
     4741 gagacctatc acacgggtaa ctggccccac cagccggtgg atttcaccgg gcgcaccgtc
     4801 ggcatcatcg gcacgggctc ctcgggcgtg caggccatac ccgtgatcgc cgcccaggca
     4861 aaggcgctcg tggtatttca gcgctcgccc aacttcagcg tgccggcccg caacgctccc
     4921 ctgccggccg atcgggccga gcgctacaag gacgagtttc tggccgcgcg cgaagcggcc
     4981 tgccggaccg acttcggcaa taccacgtgg agaagcctgc cgtcggccct ggaagccgat
     5041 ccggacgagc gcgcggcgga gtacgagcgc cgctggcagg aaggcggtgc cggcttcatg
     5101 gtcacctaca gcgacctcat gtccgaccgg gcggccaacg ataccgcggc cgaatttctc
     5161 agaggaaaga tccgcgccat cgtccgcgac cctgagacgg ccgagctgct ctcgccccgc
     5221 gaccacccca tcggggccaa gcgcatctgc gtggacaccg actactacga aaccttcaac
     5281 cgcgagaacg tgaagctcgt ggacgtacgt tctcatccca tcgagcgcat cacggcgagc
     5341 gggatcagca cgacggccgg cgactacgat ctcgactgcc tgatcttcgc taccggtttc
     5401 gatgccatga cgggcccgct cacgtccatg gacatccgcg gtcgcgatgg cgtcagcctg
     5461 agggcggcct ggcaggacgg gccgcgctgc tatctgggcc tgggcgttgc gggattcccc
     5521 aacctcttca ccatcaccgg gccgggcagc ccttccgtgc tgagcaacat ggtgctatcc
     5581 atcgagcagc acgtggagtg gattgccgac tgcctggtga agctgcgtaa ggacggcgcg
     5641 gaggccatcg aggctctccc cgacgcccag gacggctgga tcgagcacgt caacgaggcc
     5701 gccgacgcca cgctgttccc tcagggcaac tcctggtaca tcggcgccaa cgtccccggc
     5761 aaacccaggg ttttcatgcc ttataccggc ggcgtgcgcg cgtatcgcga gaagtgcgaa
     5821 gaggtggtcg gcaacagtta cgaaggcttc gacattcgcg gcggcgcgag ctgagcccgg
     5881 ccggcaactg acgtgacgag cgctgcccgg atcgcgggtc tccccagcta cttcatcgtg
     5941 ttcgtcgcgt ccatggtggc gatcgggccc tttgccatcg atacctacct gcccgccatg
     6001 ccggcgatgg cgacgaacct cggcgtggag atcgtctcgg tcaacgccac gctcagcacg
     6061 tacctcttcg gctttgcttt cgggcagatg ttcggcggcc cgatctccga tcagatcggc
     6121 cggcgccgca tcgggctgct gggcctagtc gtcttctctg ccgcgtccgt gctcatcgcc
     6181 ttggccgaat ccatagaagt catcctctgg ctgcgggcgt tgcaggccgt tggcggcggc
     6241 ttcgccacgg tgatctgcat ggccatggtg cgtgatgcct acgagcccat ggaggcggcc
     6301 aagcgctttc ccgtggtcat gcttgtcatg ctcggcgcgc ccctggcggc gcccgccatc
     6361 ggcgctgcgc tgctgagctt cggctggccg agcatcttca tcttcctcgc cctgtacggc
     6421 ctggccgtgc tcgtcgcctt cctgccggtg ccggaaaccg cttcgatgcg aacgggcaag
     6481 ctcgagctcg gccggatttt gccccagtac gccgaggtga tcacccggcg ggtcgagggc
     6541 aggcttgtgc cggcgcgcta catcctcacc cagggccttc tcatgtccgg gacgttcgtg
     6601 ttcatcacca actcctcgtt catctacctg gaatacttcg gcattgccga ggagtggttc
     6661 gtcgtctatt tcggcgtgaa catcctgatg atgatggtgt tcacattgat cactacccgg
     6721 ctgatccatc gggttgcgcc agaccgtctc tggcgcgcgg cgcgcgggct gcagtttctg
     6781 gccgtggtga tgcttgccct gggctcgtgg ttgttcgatc tgaacatctg gacgctgacc
     6841 ggcttgctgt ccgtcgtcat cggcgccaac gggatgatca acccgtccgc gtccggcctc
     6901 tacctgtcct acttcgatcg gctgagcggc agcgcggtgt cgctgatgaa cgtggccgtc
     6961 ttcctcttcg gcagcgtgct cggcgtggtc accggagtgt tcttcgacgg cacgttgaag
     7021 cccatcgtct tcaccatggc ggtggccatc accgtgggca atctgaacgc gctgggcatc
     7081 ccgccgccca agaatctggc gccggacgca agcggagagt cggcgtcggc aggctgatgg
     7141 aaaaaatcgg gcgcccgcgg gcgcccgtag tcgtgtgacc tgcgtcagcc gtcgatggtg
     7201 ccgttgcagt cgttgtcgac accgtcccgc ccccagcggc ccttggtgtc gttgtggtcg
     7261 gggtgcacgt ccgggttgtt gtcgtcacag tccacgtccg acgtgtagcc gtcggagtca
     7321 gcgtcggtcg accccgatcc gcccccgtct cctccaccgc cgcccgaccc gctggggtcg
     7381 gtaaagctgc cggtgagcgt cacgccgggg taggagctgt aggcgtagac gcccacgtac
     7441 cagtcgcctt cgagaggcgt ggcgatggcg cagctctcgc tgctggtgct gctcaccgag
     7501 cggcagtcgt agcgaacggt gtccggcagc gctgcgtgct ggacatagag atcggcgtcg
     7561 ccgcccctac ccttggccgt ctttgccagc acgaaggagg catcggtccc gcctgcggtt
     7621 acgctcagcc tgaagcagcg ccactcgccg gcgctggcgc cgaactcccc gctgctcgcg
     7681 ccgttgccca gctctgcgac ctgctcggcc gggcacacgt cacccagatt tacgtccacg
     7741 gcgctgaagg cagcctcgac gtcggcagtg gagaagccca gatccgaggc cgcatcctgc
     7801 acgcccatgg cggcggtgcg gaacgtttct ccggaggacc agtaaagctg gtttgcac
//
LOCUS       DBNHOX010000457         3725 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1322188, whole genome shotgun sequence.
ACCESSION   DBNHOX010000457 DBNHOX010000000
VERSION     DBNHOX010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1322188"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1707)
                     /locus_tag="AC16UZ_10140"
     CDS             complement(<1..1707)
                     /locus_tag="AC16UZ_10140"
                     /inference="COORDINATES: protein motif:HMM:NF023167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane phospholipid transport protein
                     YdbH family protein"
                     /protein_id="HIR6247084.1"
                     /translation="MKWLLAIVILLAGLLAGAYASLPWLAETLALREAAAVGIEVREL
                     DVRFPRWGEIAVDRLVASMQGNELRAEQLSVAYNWSGVRERRLAFVGAALLTVYVEDS
                     SGDAEPGALGEQIVVLPGLFLDAWQELPVESVQVDEVRLRTGELVFAGTFGFTPAAAS
                     LAGEVSGVEPLGALRLSMRADAAGSVGLAIQQLERPANSLAVSADARRLAGEAVIAWN
                     LELPVEDGELAVTLEGRGPIELSENGWRLGSGLQARVDGGAWRGGFAVAEAEGPWPED
                     EATELVRVSGAWQLEADLPHDVAARGTLSAGLDRSGAKLSIDPGMVVTGAAVSGASWS
                     LEGIRLDTLDGLALEVGIEPMTLTLGSPVRAALAADAARWENFALAPYVDLRIDSLAM
                     DPGGARGAFAATVPALQLSASGQIRADAESSTMVVELSARQRLRAPLLATFLAGWDQP
                     YDVDAGNVAVTGSLTLGDGALSGDVVVTLEGVQSHYGEVVAAGIAGEARVKLREDGWQ
                     ATADTLSVAVADPGFPLRDLRFAAELTPATALLSAVRGRALGGELFIDRLAYDMNDAT
                     AAF"
     gene            complement(1708..3075)
                     /locus_tag="AC16UZ_10145"
     CDS             complement(1708..3075)
                     /locus_tag="AC16UZ_10145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR6247085.1"
                     /translation="MSSGVDLSAGPVSRHLVNLTIPMFLGISSMILASMIDTVYVGWI
                     GTRELAAVSFSFPVVLAMATVSMGIGVGAASIIARVVGAGDMERVRRLSTNALYLVAM
                     LVLSLTLLGMLFVEPLFRVLGAGSDILPLSVRYMTVWFLGLPAFALPMVATNIMRAVG
                     SARLPGVIMAAGAGLQVLIAPPLIFGIPGVADGLGITGAAWSFVLSRLAMFGYTVRVM
                     QRMNLLSFAREPWSSRRESWGEVLKIGLPSMVGNLIGPVSMGVVVALLADYGHEVVGG
                     FGVASRIESLATMIIMALAASTGPVIGQNWGARRYERIYRAQSLGFRFSYLWSLLAFA
                     LLAGFGRPIVGFINQDPAVVDATYLYLLLLPATYGLLGVGMVASSTFIALGKPMPTLV
                     LSLLRMIVLYIPMALIMETLFGYAGIFAAAAVCNCLFGGLAYGWVRRMLHKEISRHGR
                     YEGAG"
     gene            complement(3093..>3725)
                     /locus_tag="AC16UZ_10150"
     CDS             complement(3093..>3725)
                     /locus_tag="AC16UZ_10150"
                     /inference="COORDINATES: protein motif:HMM:NF023284.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3376 domain-containing protein"
                     /protein_id="HIR6247086.1"
                     /translation="LERLHRLRRSQYLDGPLIEKLAVAARNLPLEPDEARDLLKDIFG
                     ALGLQSFDREFDALICTFLDRLTDDRLRDQMLTDYVGFPVFDVLLMSPASLEGGPDPL
                     TPISVERISPEDAPTLRSVFKGLKSRQFMGFLGFFNRAYREHDYLWGRLNAADRLVDL
                     LVQSSRDAISDPDRMRLKLFRAVVDRERQQLYRCDDELDRIDAFLKAAGV"
BASE COUNT          750 a         1298 c         1176 g          501 t
ORIGIN      
        1 aaaagcggcg gtggcgtcgt tcatgtcgta ggccagtcgg tcgatgaaga gctccccgcc
       61 aagcgctctg ccccgaaccg cgctgagcag cgccgtagcc ggcgtcagct ccgcagcaaa
      121 ccgcagatca cgcagaggaa agccggggtc tgcaaccgcc acgctcagcg tatcggccgt
      181 agcctgccag ccgtcttcgc gcagcttcac ccgggcctca ccggcaatgc ctgccgccac
      241 cacttcgccg tagtgggatt gaacgccttc gagggtcacg accacgtcgc ccgacagcgc
      301 cccgtcaccg agggtcaggc tgcccgtcac tgcgacgttt cccgcgtcga cgtcgtaagg
      361 ctgatcccag ccagcaagga acgtggccag cagcggcgct ctcagccgct ggcgagccga
      421 tagctcgacg accatggtcg acgactcggc gtccgcacga atctgcccag aggcggagag
      481 ctggagggcg gggaccgttg ccgcaaacgc gccccgcgcg ccgccggggt ccatggcgag
      541 ggaatcgatg cgaaggtcga cgtagggagc aagtgcgaag ttctcccagc gcgcggcatc
      601 cgcagccagc gcggcgcgaa ccgggctccc cagggtgagc gtcattggtt cgatcccgac
      661 ttcgagcgca aggccgtcga gggtatccag acggataccc tcgagactcc aagacgcacc
      721 cgaaacggcg gcaccggtca ccaccatgcc cgggtcgatg gacagcttcg cgccggatct
      781 gtccaacccg gccgacaggg ttccacgcgc cgcaacgtca tggggcaggt cagcttcgag
      841 ctgccaggcg ccggagacac ggacgagctc ggtagcctcg tcttcaggcc aggggccctc
      901 cgcttcggcc accgcgaagc ctccgcgcca agccccgccg tccacgcgcg cctgcagccc
      961 ggatcccaac cgccagccat tctcgctaag ctcgatgggg ccccggcctt ccagggtgac
     1021 ggcgagttcg ccatcctcaa cgggcagctc cagattccag gcaataaccg cctcgcccgc
     1081 cagacgccga gcatcggccg agacagccag ggagttggcc ggtcgctcta gctgctggat
     1141 ggccaggcca accgagcccg cggcatctgc gcgcatggaa agccggagcg cgccaagggg
     1201 ctcgacgccg ctcacctcgc ctgccaggct ggctgccgct ggcgtgaatc caaacgtacc
     1261 ggcaaagacg agctctcccg tgcgcaaacg gacctcatcc acctgaacgc tctccaccgg
     1321 cagttcctgc caggcgtcca ggaacaggcc cggcagcaca acgatctgct ctccaagcgc
     1381 cccgggttcc gcatcgccgc tggagtcctc gacgtacacc gtcagcagcg cagcgcccac
     1441 gaacgccagc cttcgctccc ggacgcccga ccagttataa gcgacggaca gttgctcggc
     1501 ccgcagctca ttgccctgca tgctcgcgac gagccgatcc acggcaattt caccccagcg
     1561 cggaaatcgc acgtccagct cccgcacctc gatgccgaca gcggccgcct cgcgcagggc
     1621 cagcgtctcg gccagccagg gcagcgacgc gtaagcgcct gccagcaggc cggccagaag
     1681 aatcacgatt gccaggagcc acttcaccta cccggcccct tcgtagcgcc catgacggga
     1741 aatctccttg tgcagcatcc ggcgcaccca accataggcc aggccgccga acaagcagtt
     1801 gcagacggcc gccgcggcaa agattcccgc atagccgaag agcgtttcca tgatcaaagc
     1861 cattggtatg tagagaacga tcattcgcaa cagcgaaagc accagggtgg gcatgggctt
     1921 gcccaacgcg atgaaggtcg agctcgccac catgcccaca ccgagaaggc cgtaggtagc
     1981 tggaagcaac aacaggtaga ggtaggttgc atcgacgacg gccgggtcct ggttgatgaa
     2041 gcccacgatg ggccggccga aaccggcgag gagggcgaaa gccagcagtg accacaggta
     2101 gctgaagcgg aaccccaggg attgcgcccg gtagatgcgc tcgtagcggc gagcacccca
     2161 gttctggcca atcaccggtc ccgtgctggc agccagggcc atgatgatca tggtagccag
     2221 ggactcgatg cgcgaggcga cgccaaaccc gcccaccacc tcgtgcccgt agtcggccag
     2281 cagggccacc accacgccca tggagaccgg gccgatgagg ttgccgacca tggacggcag
     2341 gccgatctta agaacttccc cccaggactc gcgacgcgaa gaccagggct cacgggcgaa
     2401 cgacaacagg ttcatgcgct gcatcaccct gaccgtgtag ccgaacatgg cgagccgaga
     2461 cagcacgaat gaccaagccg ccccggtgat tcccagcccg tccgccacgc cggggatacc
     2521 gaagatcagc ggcggcgcga tgagcacctg aaggccggca cccgcggcca tgatgacgcc
     2581 cggcaggcgc gcgctgccca ccgcgcgcat gatgttggtg gcaaccatgg gcagcgcaaa
     2641 agccggcagg ccgagaaacc acaccgtcat gtagcgcacc gacagaggca ggatgtcgga
     2701 ccctgcgccc agcaccctga agagcggctc gacgaaaagc atcccgagca gggtgaggct
     2761 caataccagc atggcgacca ggtagagcgc gttcgtcgac aggcgccgca cccgttccat
     2821 gtcgcccgcc cccaccaccc gggcgatgat ggacgccgcc ccgaccccga ttcccatgga
     2881 gaccgtcgcc atggccagga ccacgggaaa ggagaagctc accgccgcca gctcccgggt
     2941 tccgatccag ccgacgtaga ccgtatcgat catcgacgcg aggatcatcg acgagatccc
     3001 gaggaacatg gggatcgtca ggttgaccag gtggcgggat acggggcccg cggacaggtc
     3061 aacgcccgag ctcacgaatc cggtctcatc cgctagacgc ccgcggcttt cagaaacgca
     3121 tcaatcctgt cgagctcgtc atcgcaccgg tacagctgct gacgctcgcg gtcgacgacg
     3181 gcgcgaaaga gtttgaggcg catccgatcg gggtcgctga tggcatcacg agacgactga
     3241 acgagcagat cgaccagccg gtccgcggcg ttgagcctgc cccagaggta atcgtgctcg
     3301 cgataggccc ggttgaagaa accgagaaaa cccatgaact ggcgcgactt cagtcctttg
     3361 aagaccgacc tgagcgtcgg cgcgtcctcc gggctgatcc tctccacgct gatgggcgtg
     3421 agcgggtcgg ggccgccctc cagggacgcc ggcgacatga gcagcacgtc gaaaaccgga
     3481 aagccgacgt aatccgtgag catctggtcg cgcagccggt cgtcggtaag ccggtcgagg
     3541 aaggtgcaga tcagggcatc gaactcgcgg tcgaacgact gcagccccag cgcgccgaat
     3601 atgtccttga gtagatccct ggcctcgtcc ggctcgagcg gcaggttccg ggcggccacc
     3661 gcgagctttt cgatcagcgg gccgtcgagg tattgggatc gccgcagtcg atgcaggcgc
     3721 tcgag
//
LOCUS       DBNHOX010000458         2292 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1193234, whole genome shotgun sequence.
ACCESSION   DBNHOX010000458 DBNHOX010000000
VERSION     DBNHOX010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1193234"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="AC16UZ_10155"
     CDS             <1..253
                     /locus_tag="AC16UZ_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247087.1"
                     /translation="ATHEQVAGKLAKRLPPDLSVLIDLEGRWAGTHGLDTSVPNLLLF
                     AGNGQLIAREAGMFSASGFAEFATRVQDALDGGAEQAVP"
     gene            complement(250..1476)
                     /locus_tag="AC16UZ_10160"
     CDS             complement(250..1476)
                     /locus_tag="AC16UZ_10160"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6247088.1"
                     /translation="MAIFHAMVLGLLRDRGALLMSFFLPAVFFVIMAEIFTTTSGESL
                     QLNVVVKDELDDEVSGRLLSALQATPTLTLTSDPDLDRDAVRALVKSGAYDIGLIVRA
                     DGEPLDSAGGFGAAPLLIVSDPARGVAIPMLAGQIRRAYFSALPDVAMGNVIGELENQ
                     FLTLTEAQHEAVAEGLLEMRSEAEAGREVGWSFEDLLEQESVAGGKSVNLVAYSAGAV
                     AFLFLLFASVHGAVSLLEEREAGILDRVLAGPGGISIMVNGKLIYLVVQGIVQVGVIF
                     MAAWLIYDVDVPANLVEWLVVTLAASLAAAGLAMLLATACSTRRQAQTVANTVILVIS
                     ALGGSMVPRFFMPEALRDLGWLTPNTWALEAYSGIFWRQEPLTHLALPIGLLAGIGVL
                     GWQAALFLARRRAYGP"
     gene            complement(1479..>2292)
                     /locus_tag="AC16UZ_10165"
     CDS             complement(1479..>2292)
                     /locus_tag="AC16UZ_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6247089.1"
                     /translation="GKTTLLSLACGRRAPDAGRVRLAGHDPRTESPVRRQLGFVPQHV
                     ALYQHLSVVENLSVLGRMMGVARGDLAGRVAEALDWSGLADRARDRVGTLSGGMRRRL
                     NIVASLLHEPALLLLDEPTVGVDVAARERVHELLRRQRSQGLGILLTTHDLPQATELA
                     DRVVMLVDGSVRLSGVPGKLIQDTFADKRELTVTLAQPATDPQSRTLRDAGLTPGRGG
                     LTWYGAADNEHADARRWDDRLQEMALAIAEIRVRAPTLEGVYLRLTGEELDL"
BASE COUNT          417 a          765 c          762 g          348 t
ORIGIN      
        1 cgccactcac gagcaggtgg cgggcaagct cgcaaaacgt ttgccgcctg acctttcggt
       61 tctcatcgat ctcgagggcc gctgggccgg cacccacggg ctggatacca gcgtccccaa
      121 tctgctcctg ttcgccggaa acggacagct catcgcccgg gaagcaggca tgttcagcgc
      181 atccggcttc gccgagttcg ccacgcgcgt gcaggacgcg ttggacggag gcgcggagca
      241 ggccgtgccc taggggccgt aggcgcgacg ccgggcgaga aagagcgcgg cctgccaacc
      301 gaggactccg attcccgcca gaaggccgat gggcagcgcg aggtgtgtca gcggttcctg
      361 acgccagaaa atgccgctgt aggcttccag cgcccaggta ttgggggtga gccagccgag
      421 gtcacgcagc gcttcaggca tgaagaagcg gggcaccatg ctcccaccca gggcggaaat
      481 gaccaggatg acggtgttcg ccactgtctg agcctgacgg cgggtgctgc aggcagttgc
      541 gagcagcatg gctaagcccg cggccgccag ggaggccgcc aacgtgacga ccagccactc
      601 gaccaggttc gcgggcacat cgacgtcgta gatcagccag gccgccatga agattacgcc
      661 cacctgtacg atcccctgga cgacgaggta aatcagcttg ccgttcacca tgatgctgat
      721 gccgcctgga cccgcgagca cgcgatcgag gataccggcc tcgcgctcct cgagcagcga
      781 aacagcccca tggacggatg cgaagagcag gaagaggaac gccaccgcgc cggcggaata
      841 ggcgacgagg ttaacggatt tcccgccggc aacgctctcc tgttccagca ggtcttcgaa
      901 ggaccagccc acctctcggc cggcctcggc ctccgaacgc atttccagca gaccctctgc
      961 taccgcctcg tgctgggctt cagtgagtgt caggaactgg ttttccagct ccccgatgac
     1021 gttgcccatg gccacgtcgg gcagagccga gaaataggcg cgcctgatct gacccgccag
     1081 catggggatg gccacgccac gggccggatc gctgacgatc agaagcggcg ccgctccgaa
     1141 cccgccggcg gagtccagcg gctctccatc ggcacggacg atcaggccga tgtcgtacgc
     1201 gccggacttc acgagggctc tgaccgcgtc ccggtcgagg tcagggtcgc tggtcagagt
     1261 cagtgtgggg gttgcttgca gggcggagag aagcctgccg gacacttcat cgtccagctc
     1321 gtcctttacg acgacgttga gctgcaggct ctccccggac gtggtcgtga agatctcggc
     1381 catgatcaca aaaaaaaccg cgggcaggaa aaaactcatc agcagggccc ctcggtcacg
     1441 cagaaggccc aacaccatgg catgaaagat cgccatgctc acaggtcgag ctcctcgccc
     1501 gtcaggcgca gatagacgcc ctctagagtc ggggcgcgaa cgcggatctc cgcaatagcg
     1561 agcgccatct cctgcagtcg gtcgtcccaa cggcgggcat cggcgtgttc gttatctgca
     1621 gcaccgtacc aggtgagccc accgcggcct ggggtcaggc ccgcgtcgcg cagcgtccgg
     1681 gactgggggt ccgttgccgg ttgcgccagg gtgaccgtca gctcgcgttt gtcggcgaag
     1741 gtgtcttgga tcagctttcc cggcacgccg gacagacgaa cggatccatc gaccagcatg
     1801 acgacgcggt cggcgagctc ggtcgcctgg ggaagatcgt gagtggtcag cagaattccc
     1861 aggccctggg atctctgccg gcgcagcagt tcgtgcacgc gctcccgggc ggcaacatcc
     1921 acgcccacgg tgggctcgtc gagaagcagc agagccggct cgtggagcag gctggcgacg
     1981 atgttcagcc gccgacgcat cccgcccgac agggttccga ccctgtccct tgcgcgatcg
     2041 gcaagcccgc tccagtcgag ggcttcggcg acccggcccg ccaggtcgcc gcgcgccacc
     2101 cccatcatcc ggcctagaac gctgaggttt tccactacgg acaggtgctg gtacagcgcg
     2161 acgtgctggg gcacgaaccc gagctgccgg cgcaccggac tttcggttcg cgggtcgtgg
     2221 cccgccagcc gaacccggcc ggcatccgga gcgcggcggc cacaggccag ggagaggagg
     2281 gtggtcttgc cc
//
LOCUS       DBNHOX010000459         5878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1450182, whole genome shotgun sequence.
ACCESSION   DBNHOX010000459 DBNHOX010000000
VERSION     DBNHOX010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1450182"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="AC16UZ_10170"
     CDS             complement(<1..712)
                     /locus_tag="AC16UZ_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247090.1"
                     /translation="MFFSLLFVAPVAPSFANDDEYRTPYQQRTAYRAALSHLRVGNTQ
                     AFRRIESDLKGYSLHPYLEYHELRARLSSASASEVLAFRERHADLPVTPLLYQRWLER
                     LGQGREWKAFLDHWEESGDPELRCYHSRALYGTGQKEAAFAGVADLWIVGRSQPEACD
                     PLFKVWIDNDNLTEGMVWQRLDLALQANKRQLARYLQRFFSGSRKPWAQSFYNVHVTP
                     DLIARTDRFETDNELSRAV"
     gene            complement(795..1259)
                     /locus_tag="AC16UZ_10175"
     CDS             complement(795..1259)
                     /locus_tag="AC16UZ_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6247091.1"
                     /translation="MADYRRIMLAVDLTEESNHVAERAIAIAKAFDAELHLVHVIEPL
                     SLAYGGDIPMDLSSVQEQIHDQAKSHLAEFANRLGVPAERQNLIFGRPESEIHRVAES
                     SEADLIVVGSHGRHGLALLLGSTANGVLHGATCDVLAVRVGSIAEKSPDSDS"
     gene            1333..1905
                     /locus_tag="AC16UZ_10180"
     CDS             1333..1905
                     /locus_tag="AC16UZ_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247092.1"
                     /translation="MTWLIIGLILLAAFGPVLWLVPSRKDRHLSALREQARLEGLVVE
                     LRRLPKLQPSAHERVTAGGRVKKPVQECAAYMQMLRRRLVMLPGWRVHRGTGDLEARG
                     GWYFDFELRARGDAFDATMSALDPLFGGLPADVVAVELTDRTLLAYWLERGAATRETV
                     SEMALQMRAAEQALLDLDARFQSLAENDDS"
     gene            1970..4648
                     /gene="topA"
                     /locus_tag="AC16UZ_10185"
     CDS             1970..4648
                     /gene="topA"
                     /locus_tag="AC16UZ_10185"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR6247093.1"
                     /translation="MPPSLVIVESPAKAKTINRYLGSDYIVRSSVGHIRDLPVGGKSV
                     DPKARAREAAKTRKLSPEKKAEYKQERARQQLVRRMGVDPDKGWAAEYEILPGKEKVV
                     DELTKLAKNAEAIYLATDLDREGEAIAWHLRETIGGDPARYRRVTFNEITRRAIQEAF
                     SEPSELDMNQVHAQQARRFLDRVVGFELSPLLWGKIARGLSAGRVQSVAVRLVVERER
                     EIRAFIPEEYWEAFADLKRGADGDVLRFQLIGDGGSNFRPDNGEDAHEALGRLKDREL
                     VVRSREDKPTRTKPGAPFITSTLQQAASTRLGFSVKKTMTLAQRLYEAGFITYMRTDA
                     TNLSADAVAACRDYIGEQHGAEYLPDQPRTYASKEAAQEAHEAIRPSNVRTLAANLSG
                     VDDDAVRLYDLIWRQFVACQMPEARYLSTTVVVEAGELELRVRGRIMQFDGYTRVQPP
                     SSRREEDVNLPDYAIGEKLALVHAEAVQHFTKPPPRYGEASLVRELEKRGIGRPSTYA
                     AIISTIQDRGYVTLRNRRFHAEKIGELVTDRLLEKFENLMDYGFTAQMEGELDRIAEG
                     EIGWQAVLDEFYGDFSDKLVRAQSADDGMRRNEPSDTDIKCARCGRVMQVRTASTGVF
                     LGCSGYNLPPKERCTNTMNLIPGEEAVDVEQDAEAESKLLRQKLHCPRCGTAMTAYLI
                     DEARKLHVCGNNPDCAGFVVEAGQFRIKGYEGPVIECDKCGADMQLKSGRFGKYFGCS
                     SDECSNTRKLLRNGEPAPPKADPVPMPELRCEKVDDHYLLRDGASGIFLAASQFPKNR
                     ETRAPLVREIKPHGNELDPKFNYLLDAPEEDPEGRPTVIRYSRKSREQYVQSELDGKA
                     TGWKAFYRDGRWVEENQPSTRSRKSAARKSSKARAG"
     gene            4708..4932
                     /locus_tag="AC16UZ_10190"
     CDS             4708..4932
                     /locus_tag="AC16UZ_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247094.1"
                     /translation="MVFEITQLVDGDVVLREADDEGEPLVRIRFSAEVKDMLGDDAVV
                     VAEAMIDAAAAFLEADPEDQDLTAPAPVLH"
     gene            complement(4940..5539)
                     /gene="lexA"
                     /locus_tag="AC16UZ_10195"
     CDS             complement(4940..5539)
                     /gene="lexA"
                     /locus_tag="AC16UZ_10195"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR6247095.1"
                     /translation="MTNLTPRQAQVLDLIKQHISDTGYPPTRADIAQELGFKSPNAAE
                     EHLKALSRKGAIEMIPGTSRGIRLPEETGLPVIGRVAAGNPILAEENIEDHVDIPSGF
                     FHPRADYLLRVRGDSMIDAGILDGDLLAVHKTPEAQNGQIVVARVEDEVTVKRFQRGR
                     NRSRVTLLPENSNYAPIEVDLQAQPFAIEGLSVGVIRRS"
BASE COUNT         1117 a         1789 c         1963 g         1009 t
ORIGIN      
        1 tcacggctct ggaaagctcg ttgtccgtct cgaagcggtc agtccgcgcg atgaggtctg
       61 gcgtgacgtg cacgttgtag aacgattgag cccacggctt tcgtgaaccg gagaagaagc
      121 gctgaagata ccgggcgagc tgacgtttgt tggcctgtag cgccagatcc aggcgctgcc
      181 agaccatgcc ctccgtcaag ttgtcgttgt cgatccacac cttgaacaac ggatcgcacg
      241 cttccggctg ggacctgcct acgatccaca ggtccgcaac gcctgcgaaa gcagcctcct
      301 tctggccggt gccgtaaagg gcccggctgt gatagcaacg cagctccgga tcgccgcttt
      361 cctcccagtg gtcgagaaac gccttccact ctcgcccctg gccgagtcgc tccagccagc
      421 gctggtagag aagcggggtg accggaaggt cggcgtgccg ctcgcgaaat gccagcacct
      481 cgctcgccga agcgctggac aggcgagcgc ggagctcgtg gtactccaga tacgggtgta
      541 gcgagtaacc cttcaggtcc gactcgatgc ggcgaaacgc ctgcgtattg ccgacccgca
      601 ggtgcgaaag agcggcccga tacgcggtgc gctgctggta tggggtgcgg tactcgtcat
      661 cgttggcgaa cgacggcgcc accggcgcca cgaacaacag cgagaagaac atcaggtgcc
      721 agcgccgaca gtggccgggc agagcggtgc tgggtgacgg gagggtcagc cggcccctta
      781 gactggcaat cagatcagct atcggaatcc ggcgacttct ccgcgatgct gcccacgcga
      841 acggccagaa cgtcgcaggt ggcgccgtgc agcacgccgt tggcggtgga gccgagcagc
      901 agggcaaggc cgtgccggcc gtggcttccc accacgatga ggtcggcttc gctggattcg
      961 gcgacgcgat ggatctcact ctcggggcgt ccgaagatga ggttctggcg ctctgccgga
     1021 acgcccagac ggttggcgaa ctcggcgaga tgggatttag cctgatcgtg gatctgctcc
     1081 tgaacagacg acaggtccat gggaatgtcg ccaccgtagg caaggctcaa tggttcgatg
     1141 acatgcacga gatgaagttc cgcgtcgaag gccttcgcaa tagcgatagc ccgctccgcg
     1201 acgtgattgg actcctcggt gagatccact gccaacatga ttcgacggta gtcagccatg
     1261 ttgcctcccg gcacgaattg cggcgacgat acacacaatc caaaggcccc tcaacctctc
     1321 ggggagcgtc ccatgacgtg gttaatcatc ggtttgatcc tgctagccgc cttcggtccg
     1381 gtgctctggc tggtgcccag cagaaaagac cgccacctca gcgcgttgcg agagcaggcc
     1441 cgactcgagg ggctcgtggt ggaattgagg cgattgccca agctgcagcc gtccgcccat
     1501 gagcgggtca cggccggggg acgcgtcaag aaaccggtgc aggagtgcgc ggcgtacatg
     1561 cagatgctgc ggcgaagact cgtgatgctg cccggctggc gcgtccatcg tggcaccggg
     1621 gatttggaag cccgcggcgg ctggtatttc gacttcgagc tccgggcacg gggggatgca
     1681 ttcgacgcga cgatgtccgc tctggaccct cttttcgggg ggctccccgc tgacgtcgtc
     1741 gcggtggagt tgaccgaccg aacgctgctc gcctactggt tggaacgagg cgctgccacc
     1801 cgggaaaccg tgtccgagat ggccttgcag atgcgagcag cggagcaagc gctgctcgat
     1861 cttgatgccc gttttcagag cctggcggag aacgacgatt cttgacagcc gttttggcca
     1921 ggccgtataa cggcgcccga acccatcctc gggcgctgtt caggcacata tgcccccttc
     1981 tttagtcatc gttgaatcac cggccaaggc caagacgatc aatcgttacc ttggcagcga
     2041 ctacatcgtg cgctccagcg tgggtcacat tcgggatctg ccggtgggcg gcaagagcgt
     2101 cgatcccaag gcgcgagcca gggaggctgc caagacgcgc aagctgtctc cggagaaaaa
     2161 agcggagtac aaacaggagc gggcccgcca gcaactggtc agacgcatgg gcgtagaccc
     2221 ggacaagggc tgggcggccg aatacgagat cctgcccgga aaggaaaagg tcgttgacga
     2281 gctcaccaag ctcgccaaga acgccgaggc catctacctc gccaccgacc tggatcgcga
     2341 aggggaggcc atcgcctggc atctcagaga gaccatcggc ggcgatccgg ctcgttatcg
     2401 gcgggtgacg ttcaacgaaa taacccggcg ggccattcag gaggccttct ccgagccgtc
     2461 agagctcgac atgaaccagg tgcacgctca gcaggccagg cgattcctgg accgtgtcgt
     2521 cggcttcgag ctctcgccgc tgctgtgggg gaagattgcc cggggcctgt cagccggtcg
     2581 ggtgcagtcg gtggccgtgc ggctggtcgt ggagcgggag cgggaaatcc gggccttcat
     2641 ccccgaggag tactgggagg ctttcgccga tctcaagcgc ggggctgacg gcgatgtgct
     2701 cagattccag ctgataggcg atggcggcag caacttccgg ccggacaacg gcgaggatgc
     2761 ccacgaggcg ctggggcgtc tcaaagaccg ggagctggtg gtcaggagcc gtgaggacaa
     2821 gcctacccgc accaagcccg gcgcgccctt catcacgtcc acccttcagc aggcggccag
     2881 cacgcgcctc gggttcagcg tgaaaaagac catgacgctg gcccagaggc tctatgaggc
     2941 aggcttcatt acctacatgc gcacggacgc caccaatctg agcgccgatg ccgtggccgc
     3001 ctgtcgcgac tacatcggcg agcagcatgg cgccgaatat ctgcccgacc agccgcgcac
     3061 atatgcaagc aaggaggccg cgcaggaggc tcacgaagcc atccggccgt ccaacgtgcg
     3121 aacgctggct gccaatctca gtggagtcga tgacgacgcc gtgcgcctgt acgacctgat
     3181 ctggaggcag ttcgtcgcgt gccagatgcc ggaggcccgc tatctcagca ccacggttgt
     3241 cgtagaggcc ggcgagctgg agctgcgggt tcgtggccgg atcatgcagt tcgacggcta
     3301 caccagggtg caaccgccgt cttcgcgcag ggaggaggac gtcaatctgc ccgactacgc
     3361 gatcggggag aagctcgctc tggttcatgc ggaggccgtg cagcacttca ccaagccgcc
     3421 gccgcgatac ggcgaagcca gcctggtacg agagctcgaa aagcgtggga tcgggcggcc
     3481 ttccacctac gcggccatca tctccaccat tcaggatcgc gggtacgtga cgctgcggaa
     3541 ccgcaggttc catgccgaga agatcggcga gctcgtgacc gaccggttgc tggagaagtt
     3601 cgaaaacctg atggactacg gtttcaccgc gcagatggaa ggggagctcg accgcattgc
     3661 cgagggtgag attggctggc aggccgtgct ggacgagttc tacggggatt tcagcgacaa
     3721 gctcgtgcgg gctcagagcg cggacgacgg catgcgtcgg aacgagccgt cagacaccga
     3781 catcaagtgc gcgcgctgcg ggcgcgtgat gcaggttcga accgccagca cgggcgtgtt
     3841 tctgggctgc tcggggtaca acctgccgcc gaaggagcgc tgtaccaaca ccatgaacct
     3901 gattcccggc gaagaggccg tggacgtcga gcaggatgcc gaggccgaat ccaagctgct
     3961 ccggcagaag ctccattgcc ccaggtgcgg gaccgccatg accgcctacc tgatagacga
     4021 ggcccgcaag ctgcacgtgt gcggcaacaa tccggattgt gccggcttcg tggtggaggc
     4081 cggccagttc cgcataaagg gctacgaggg cccggtgatc gagtgcgaca agtgcggcgc
     4141 ggacatgcag ctcaagtcgg gccgtttcgg caagtacttc gggtgcagca gcgacgagtg
     4201 cagcaacacc cgcaagctgc tgcgcaacgg cgagccggcg ccgcccaagg cggatccggt
     4261 gcccatgccc gagctgcgct gcgagaaggt ggacgaccac tacctgctca gagacggagc
     4321 gtcgggcatt tttcttgccg ccagtcagtt tcccaaaaac cgggaaacgc gcgcgccgct
     4381 ggtacgggaa atcaagcccc acgggaacga gctcgatccg aagttcaatt atctgctcga
     4441 cgcgccggaa gaggatccgg aagggcgccc gaccgtcatc cgctacagcc gcaagagccg
     4501 cgagcagtac gttcagtccg agttggatgg caaagcgaca ggctggaaag ccttctatcg
     4561 cgatggtcgg tgggttgagg aaaaccagcc gtcgacccgg tcgcgcaagt cggccgcgcg
     4621 caagtcgagc aaagcccgcg caggttagct gcgttgagca tcgggcagag ttcgagcaaa
     4681 ttcagacgca cagggagcta atctggaatg gtgtttgaga tcacgcagct cgtcgatgga
     4741 gacgtggtcc tcagagaggc ggacgacgag ggagaacccc tcgtgcgaat ccggttctct
     4801 gccgaggtca aagacatgct gggggatgat gcggtggtgg ttgcggaggc gatgatcgat
     4861 gcggctgccg ctttcctgga ggcggatccc gaagatcagg acctcaccgc gccggctccc
     4921 gttctgcact gacgccgagt cagcttctgc ggatcacccc cacgctgagc ccttcgatgg
     4981 cgaaaggctg agcctgcaga tcaacctcga tgggcgcgta gttcgaattc tccggcagca
     5041 gcgtcaccct gctgcggttc cggccccgct ggaagcgctt caccgtgacc tcgtcctcca
     5101 ctcgggcgac gacgatctgg ccgttctggg cttccggcgt cttgtgcacc gccagcagat
     5161 ccccgtcgag aatgcccgca tcgatcatgc tgtcgccgcg cacgcgcagc agatagtcgg
     5221 cgcgggggtg gaagaacccg gacggaatgt ccacatggtc ctcgatgttc tcctcggcaa
     5281 gaatcgggtt gcccgccgca accctgccta tcacgggaag ccccgtctcc tccggcagcc
     5341 ggatcccgcg tgaggtgccg ggaatcatct cgatggcgcc cttgcgcgag agtgccttga
     5401 gatgctcttc ggcggcgttg ggggatttga agcccagttc ctgggcgata tccgcgcggg
     5461 tcggcgggta gccggtgtcc gagatgtgct gtttgatgag gtcgagaacc tgagcctgtc
     5521 ttggggtgag gttggtcatt tgcggtcaat cgagcctggt tgaatataca gtgggctgtg
     5581 agtatataca gtatccgagc ggtttgcacg gtccccgggg gttacttaat atccgagccg
     5641 ccgtccatcc cccggaagcc ccgtgggccg cgggggcctc ttgcgaagga gcggaagagg
     5701 agttcatgag ccgccccatc atcgtctgtc tagtcattct cattcttctg ccgtggggct
     5761 tgcgctccgc ggcggccgtg gatctgcgcc tcgacggtct gaccgccgag cagtccggcc
     5821 gagccatatt cgaggctgca gaccagctcg aggacgggta tgccgacatg caggtgga
//
LOCUS       DBNHOX010000460         5624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1322289, whole genome shotgun sequence.
ACCESSION   DBNHOX010000460 DBNHOX010000000
VERSION     DBNHOX010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1322289"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..100
                     /locus_tag="AC16UZ_10200"
     CDS             <1..100
                     /locus_tag="AC16UZ_10200"
                     /inference="COORDINATES: protein motif:HMM:NF024855.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HIR6247096.1"
                     /translation="GYAPGVRMSFNGEAVVLTPHTRNAVADLVLGQ"
     gene            103..1371
                     /gene="hisS"
                     /locus_tag="AC16UZ_10205"
     CDS             103..1371
                     /gene="hisS"
                     /locus_tag="AC16UZ_10205"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR6247097.1"
                     /translation="MVQSIRGMRDVLPEEGRRWRVVEDAIRAVLTTHAYEEVHLPLLE
                     ATELFSRGVGEATDIVEKEMYTLADRDGDSISLRPEGTAVCARALLQHGLLHNQTQRV
                     FYAGPMFRYERPQKGRYRQFYQIGAEAFGLAGPDVDAELILMCQLMWKALGISGETRL
                     EINTLGSGGARAAYRAALVGFLTPLAAGLDADSQRRLGSNPLRILDSKVEQTQDLLAG
                     APSLEDFIDDEGKAHFSGLCELLEQLDVPFEINPKLVRGLDYYTHTVFEWKTDALGAQ
                     DTICAGGRYDGLVELLGGRPTPGAGFALGVERVLLLHEQRFQGAGFAAAADVYCCVLD
                     DRRQSEVFAIAQKLRDALPGLRIRTHAGGGKLKSQMRRADASGARLAVLVGEDEIAKG
                     VVSLKYLRESREQESVPAADLETVLAEYFA"
     gene            1403..2104
                     /locus_tag="AC16UZ_10210"
     CDS             1403..2104
                     /locus_tag="AC16UZ_10210"
                     /inference="COORDINATES: protein motif:HMM:NF021492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0044877 - protein-containing complex
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HIR6247098.1"
                     /translation="MSDYLTDEEQVAKLKSWWDENGVSVIVTLVLVVAGVVGWRWYES
                     YHETRVARVSDLYEEFLVSEGDARDASAASIAEEAPDSAYQTLVMLRRSQESMESGDY
                     EAAEAFLVEAVEAAPEPVLADVARVRLARVQQQLDRLDDALETLSGVLGEGYRSQVAE
                     MKGDIHLARGERALAYEAYASALAAVGDNQSRPVLEMKAADTADAVTGAQPAAAPEAG
                     QGLESPGETAETPDA"
     gene            2097..3323
                     /gene="bamB"
                     /locus_tag="AC16UZ_10215"
                     /gene_synonym="yfgL"
     CDS             2097..3323
                     /gene="bamB"
                     /locus_tag="AC16UZ_10215"
                     /gene_synonym="yfgL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019408675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HIR6247099.1"
                     /translation="MRKILLLLLTFSLTGCSWFSWLPWVGDKDDEKKKLEPAKLVDFD
                     AEVNVDRVWRASIGEGLGKKYLRLDPLVLADRVYAADGYGIVEALDRFSGKRVWRSRI
                     GRVEEGLLGSINFFDREDPSFVSGGVGGGAGYVFMGTTGGDVVALSAADGSEAWRSHV
                     GSEVLAPPVGERDLVFVQTQDGRLLALESETGDIRWSFDNQVPVLTLRGTGTPVVAGN
                     VVFAGFSNGMLAALRAESGEPVWEQRIMLPEGRSELERMVDVDGTPLLLGPILYVVAY
                     QGKLKALRRTDGAMLWEMDVSSFLDLAEGYGQIYVVDDGDVVTAVDQQSAEIVWQQPG
                     LARRKLTSPVAFSNYVAVGDDQGYLHILAQSDGRFVGRRKLDGDGLRSSMAVVDGVLY
                     VLGNSGSLHALDIEVK"
     gene            3331..4737
                     /gene="der"
                     /locus_tag="AC16UZ_10220"
     CDS             3331..4737
                     /gene="der"
                     /locus_tag="AC16UZ_10220"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR6247100.1"
                     /translation="MVPTVALVGRPNVGKSTLFNKLTRRRDALVADVPGLTRDRRYGR
                     MPAGAGECMLIDTGGLFGEPGALQSALEQQAELALDEADAVLFVTDARDGLVPADEEI
                     AQRLRRLSKPVILVVNKMDGVQADVAMADFAALGFDAVAYVSAVHERGLGTVRELVEE
                     RCPHGPTGEEAADESDAVRVAVIGRPNVGKSTLVNRMLGQERQVVFDEPGTTRDAIEV
                     PFEKDGKRYVLVDTAGVRRKGRVEGVAEKFSVVKTLQAIDLAHVVILVLDAREGVVDQ
                     DLHLLSHAAEAGSGLVVAMNKWDGLSVEERTNARRVLDRKLSFAPWIPVMQISALHGT
                     GVGHLLEEVDRVYKAGELDVSTSVLTRLLLGMTGAHPPPAVRGRSIKLRYAHKAGSHP
                     PRIVIHGNQTEAVPDSYRRYLENGFRKALALVGNPVHIELRTGDNPYAGQRNELTPRQ
                     KARRKRMIQHRKRKAKKR"
     gene            complement(4775..5575)
                     /locus_tag="AC16UZ_10225"
     CDS             complement(4775..5575)
                     /locus_tag="AC16UZ_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR6247101.1"
                     /translation="MLARAALATLCWLTPMLLRADAVPGGLYAYGPVPPASEVRFDDR
                     RVLVIDETALVGIPLSASPGTHSLTITYASGESVSHQFEVVAKQYPEQRLTITDRKMV
                     NPDPEDLERIRTESARMREQYLSFTEAMQRLRPFHKPVNGVTSSPFGRRRILNDQPRN
                     PHSGLDIAADHGTPVSAPAPARVSLTGDFYFNGKTIFLDHGQGLVTMYCHLSEISVEP
                     GEAVERGATIGLVGATGRATGPHLHWSVSLNGNRVDPELVISALAVGD"
BASE COUNT          957 a         1714 c         1965 g          988 t
ORIGIN      
        1 cgggtacgcg cccggcgtgc gcatgagttt caacggcgag gccgtggtgc tcacgcccca
       61 cacccgcaac gccgttgcgg acctcgtgct tggtcagtag cgatggtcca gtcgatcaga
      121 ggcatgcgcg acgtgctgcc cgaagagggc aggcgctggc gggtcgtgga agacgccatc
      181 cgcgcggtgt tgaccacgca cgcctacgaa gaggttcacc tgcccctgct cgaggccacg
      241 gagctgttca gccgtggcgt gggcgaggcc acagacatcg tcgagaagga gatgtacacc
      301 cttgccgacc gggacggaga cagcatctct ctgcgccccg agggcacggc cgtctgcgcc
      361 cgcgcgttgc tccagcacgg tctgctccac aaccagaccc agcgcgtttt ctatgcaggc
      421 cccatgtttc gctacgaacg tccgcagaag ggccgctatc ggcagttcta ccagattggc
      481 gccgaggctt tcgggttggc ggggccggac gtggatgcag agctgatctt gatgtgccag
      541 ctcatgtgga aggctctggg aatctccggc gaaacccgcc tggagatcaa taccctcgga
      601 tcggggggtg cgcgggccgc ttatcgggca gctctggtgg gctttctaac gccgctggcg
      661 gccggtctgg atgctgacag ccagcgccgg ctcggctcga acccgcttcg cattctggac
      721 agcaaggtcg agcagaccca ggacctgctg gccggggcgc cctccctgga ggacttcatc
      781 gacgacgaag gaaaggcgca cttctccggt ctgtgcgagc tgctcgagca gctcgatgtt
      841 ccgtttgaaa tcaatccgaa gctggtccgt ggcctggatt actacaccca caccgtgttc
      901 gagtggaaaa ccgacgcgct cggagcccag gacacgatct gtgcgggtgg ccgttacgat
      961 gggctggtcg agctgctcgg cgggcggccg acgccgggcg cgggctttgc cctgggggtg
     1021 gagcgcgttc tgctccttca cgagcagcgg ttccaggggg ccggcttcgc ggctgcggcc
     1081 gacgtttact gctgcgtgct ggacgaccgg cgccaatcgg aggtattcgc catcgcgcag
     1141 aaattgcgtg atgcgctccc aggtctcaga atccgcaccc acgccggtgg cggcaagctg
     1201 aagagccaga tgcgacgcgc cgatgccagc ggcgcgcgcc tcgcggttct cgtcggcgag
     1261 gacgagatcg ccaagggcgt ggtgtcgctg aaataccttc gcgagagccg ggagcaggaa
     1321 agcgtcccgg ccgctgatct ggaaacggtg ctggccgagt acttcgcctg aacctggcga
     1381 gcgaatcgaa aaggagattg gggtgtcgga ttacctgacc gacgaagagc aggtcgcaaa
     1441 gctcaaatca tggtgggacg agaacggcgt gtccgtcatc gtgacgctcg ttctggtcgt
     1501 cgccggggtg gtcggttggc gatggtacga aagctaccat gagacgcggg tcgcgcgagt
     1561 ttcggatctc tacgaggagt ttctggtcag cgaaggggac gccagggacg caagcgccgc
     1621 gtccatcgcc gaagaggcac ccgacagtgc ctatcagacg ctcgtcatgc tgcgccgctc
     1681 gcaagagtcc atggagagcg gcgactacga agccgccgag gcgttcctcg tcgaggcggt
     1741 ggaagctgct ccggaacccg ttctggcgga cgttgcccgg gtgcggctcg cgcgggtgca
     1801 gcagcagctg gaccgtctcg atgatgcgct cgaaacgctt tccggggtgc ttggcgaagg
     1861 atatcggtca caggttgccg agatgaaggg ggatatccat ctggcccgcg gggagcgcgc
     1921 ccttgcttac gaagcatacg cttcggcgct tgcggcggtg ggtgacaacc agtcccgtcc
     1981 ggtgctggag atgaaagccg ccgataccgc cgatgccgtg accggagcgc agcccgctgc
     2041 agcgccggaa gccggacagg gcctcgaatc ccccggggag actgcagaaa cgcccgatgc
     2101 gtaaaattct actgctcctg ctgactttct cccttaccgg ctgcagctgg ttttcctggt
     2161 tgccgtgggt gggcgacaag gacgacgaga agaaaaagct cgagccggcc aagctggtgg
     2221 acttcgatgc cgaggtgaac gtcgaccggg tctggcgggc ctccatcggt gagggtctcg
     2281 gcaagaagta tctgcgactt gatcccctgg tcctcgccga ccgggtgtac gccgcggacg
     2341 gctatggcat cgtggaagcg ctcgaccgtt tttccggcaa gcgggtctgg cggtcgcgca
     2401 taggtcgcgt cgaagaaggt ctcctgggct caatcaactt cttcgatcgt gaagatccga
     2461 gtttcgtgag tggcggggtc ggcggtggtg caggctacgt tttcatgggc acgacgggcg
     2521 gtgatgtggt ggcgctgtcc gctgcggacg gttcggaagc ctggcggtcc cacgtgggca
     2581 gcgaggtgct cgcgccaccg gtgggcgagc gcgatctggt gttcgtgcag actcaggacg
     2641 gcaggctgct ggcgctggaa tccgagaccg gggacattcg ctggagcttc gacaaccagg
     2701 ttccggtgct gaccttgaga ggcaccggca ccccggtggt ggccggtaac gtggtgtttg
     2761 cgggcttttc caacgggatg ctcgccgctc tgcgcgccga aagcggcgag cccgtctggg
     2821 agcagcggat catgcttccg gaaggaaggt ccgagctgga acgcatggtg gacgtggacg
     2881 gcacgcccct gctcctgggt ccgatcctct acgtcgtggc ttaccagggt aagctcaagg
     2941 cgctgcgacg aaccgacgga gccatgctgt gggagatgga cgtgtccagc ttcctggatc
     3001 tcgccgaagg atacggccag atctacgtgg tggacgacgg ggacgtggtg acggccgtcg
     3061 accagcagtc ggcggagatc gtctggcagc agccgggcct tgcgcgtcgt aagctcacga
     3121 gccccgttgc tttcagcaac tatgtcgcgg tgggcgacga tcagggatat ctgcacatcc
     3181 ttgcgcagag tgacgggcgc ttcgtcgggc gccgcaagct cgacggggac gggctgcggt
     3241 cgtcgatggc ggtcgtggac ggcgtgcttt acgtgctcgg aaactccggt tccctccatg
     3301 cgctcgacat tgaggtcaaa taggcccggg atggttccca ccgtcgccct cgttggccgg
     3361 cccaacgtcg gtaagtccac actcttcaac aagctgacca ggcgccggga cgctctggtg
     3421 gccgacgttc ccggcctgac ccgggatcgg cgctacgggc gcatgccggc aggggccggt
     3481 gagtgcatgc tcatcgacac cggggggctc ttcggggaac cgggcgcgct gcagtcggcc
     3541 ctcgagcagc aggccgagtt ggcactcgac gaagccgacg cggtgctgtt cgtgaccgat
     3601 gcccgggacg gcctggtgcc cgctgacgag gagatcgccc aacgcctgag gcgtctttcc
     3661 aaaccggtga ttctggtggt caacaagatg gatggcgtgc aggcggacgt tgcgatggcc
     3721 gactttgccg ccctcggctt cgatgccgtg gcctatgtct ctgcggtgca cgagagggga
     3781 ttgggcaccg tacgcgagct ggtggaggag cggtgccccc acggcccgac tggggaggaa
     3841 gcagccgacg aatcggatgc cgtgcgcgtc gcggtgattg gccgccccaa cgtggggaaa
     3901 tccacgctgg tgaaccgcat gctcggccag gagcgccagg tggtgttcga tgaacccggc
     3961 accacgcgtg acgccatcga ggtgcctttc gagaaggacg gcaagcgcta cgttctggtg
     4021 gataccgccg gcgtgcggcg caaagggcgg gtcgaggggg tggccgagaa gttctcggtg
     4081 gtgaagaccc tgcaggccat cgatctggcc cacgtggtca tactcgtcct cgacgcccgg
     4141 gaaggggtgg tggatcagga tctgcacctg ctcagccatg ccgccgaggc gggctcgggg
     4201 ctggtggtgg ccatgaacaa gtgggacggg ctctcggtcg aggaaaggac gaatgccaga
     4261 cgggttctgg accggaagct gtcttttgca ccctggattc cggtgatgca gatatcggcc
     4321 ctgcacggca ccggcgtggg ccaccttctc gaagaggtcg accgggtcta caaagcggga
     4381 gagctcgacg tcagcacgtc tgtgctgacc cggctgctcc tcgggatgac gggcgcccac
     4441 ccgccacccg cagtccgtgg ccgttccatc aagctgcgct acgcccacaa ggccggatcg
     4501 catccccccc ggatcgtgat ccacggcaac cagaccgaag cggttccgga cagctatcga
     4561 agatacctgg aaaacggctt ccggaaggct ctcgccctgg tgggcaaccc cgtgcacatc
     4621 gagctgcgca ccggagacaa tccttatgcc ggccagcgta acgagctcac gccccgtcag
     4681 aaagcccgtc gcaaaaggat gattcagcat cgcaagcgca aagcaaaaaa acgctgaccg
     4741 gcgtcctctc ccgcccggtg gtgccgaagg tgggctagtc cccgacggcc agggcagaga
     4801 ttaccagctc gggatcgacc cggttaccgt ttaggctgac cgaccagtgc aggtggggtc
     4861 ccgtggcccg cccggtggcg cccaccagac ctatggtggc gcccctttcc accgcctcac
     4921 cgggctccac gctgatttcg ctcaggtggc agtacatggt caccagtccc tgtccgtgat
     4981 cgaggaagat cgtcttgccg ttgaaataga agtcgcccgt gagactgacc cttgcaggtg
     5041 ccggagcgct cacgggggtg ccatggtcgg cggcgatgtc cagaccgctg tgcgggttgc
     5101 ggggctggtc gttgaggatg cgccggcggc cgaaggggct tgaggtcact ccgttcaccg
     5161 gcttatggaa cggacgcagc ctctgcatgg cttcggtaaa gctcaggtac tgctcgcgca
     5221 tcctcgcgct ttctgtccgt atgcgctcga ggtcctccgg gtccggattg accattttcc
     5281 gatcggtgat agtcaggcgc tgctccgggt actgctttgc caccacctcg aactggtgcg
     5341 agaccgactc tccggacgca taggtgatgg tcagcgagtg cgtgccgggt gatgcagaca
     5401 gcgggatgcc cacgagagcg gtctcgtcga taaccagaac ccgacgatcg tcgaaacgca
     5461 cctccgaggc gggcggcacg ggcccgtagg cgtacaaacc gccaggcacc gcgtcggccc
     5521 gcaggagcat cggcgtcagc cagcacagcg tggccagcgc ggcgcgggcg agcatctagt
     5581 gctcctccac gtgggtttgc gccgcctgca gcgcgctttc ttcg
//
LOCUS       DBNHOX010000461         1513 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1515298, whole genome shotgun sequence.
ACCESSION   DBNHOX010000461 DBNHOX010000000
VERSION     DBNHOX010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1515298"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="AC16UZ_10230"
     CDS             <1..315
                     /locus_tag="AC16UZ_10230"
                     /inference="COORDINATES: protein motif:HMM:NF042896.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HIR6247102.1"
                     /translation="YHLLLTQVEARRDWIQGTVTLDVVGRRTPDPSRKDVELVLSLTE
                     IGQTDTYPLSFRFRYFQDLTGSMALPDGFLPEAVVVTARKRGARSEQLQRKFAWAMGE
                     AE"
     gene            377..826
                     /locus_tag="AC16UZ_10235"
     CDS             377..826
                     /locus_tag="AC16UZ_10235"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR6247103.1"
                     /translation="MARTKVRDGVTLIAANTEVTGNVKFNGQLYVSGRVNGNILAAEG
                     CEATVVVSEEGVVSGEIRVPNVVINGTVEGDISASERIELACKARVKGSVYYALVEMQ
                     LGAQVDGQLVHEHPQTAASEDAHKAGAKRRNPLGIIKQLPKAGATAS"
     gene            941..1285
                     /gene="erpA"
                     /locus_tag="AC16UZ_10240"
     CDS             941..1285
                     /gene="erpA"
                     /locus_tag="AC16UZ_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009408529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HIR6247104.1"
                     /translation="MTGQLDIDISFTERAAAKVRALIADEENADLKLRVFITGGGCSG
                     FSYGFTFDEEVAEDDAVVEREGVSMLVDSLSYQYLLGAEVDYKEGLQGAQFVVQNPNA
                     SATCGCGNSFAI"
     gene            complement(1304..>1513)
                     /locus_tag="AC16UZ_10245"
     CDS             complement(1304..>1513)
                     /locus_tag="AC16UZ_10245"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HIR6247105.1"
                     /translation="RHNRLLMERLAQLTPSPIETTDAHGVDGDALEAGAFAWLAHQTM
                     ASLPGNAPGVTGASGERILGGIYRP"
BASE COUNT          310 a          450 c          493 g          260 t
ORIGIN      
        1 tatcacctgc tgctgaccca ggtggaggcc cgccgggatt ggattcaggg caccgtgacc
       61 ctcgatgtgg tgggccgaag aacgccggat ccgtcacgga aagatgtaga actggtgctc
      121 tccctcacag aaatcgggca aaccgacaca tatcctctga gtttccggtt ccggtacttc
      181 caggacctca ccgggagcat ggcgttaccc gatggatttc tgccggaagc ggtggtggta
      241 accgcccgga aacgcggtgc gcgctccgag cagttgcagc gaaagtttgc ctgggccatg
      301 ggcgaagccg aatgagcgcc gtttcctaaa cggacagcga gagcgcgcca gggcccccca
      361 ggaacgaagg gtcatcatgg cgagaaccaa ggtccgagac ggcgtaaccc tgatcgcagc
      421 gaataccgaa gtcaccggga acgtgaagtt caacgggcag ctctacgtca gcggtcgggt
      481 gaacggcaac attctcgccg ccgaaggctg cgaagccacc gtggtggtga gcgaggaggg
      541 tgtggtttcc ggcgagatcc gcgttcccaa cgtggtcatc aacggcaccg tcgaaggcga
      601 catctctgct tccgagcgga tagaacttgc ctgcaaagcc agagtcaaag gaagcgtgta
      661 ttatgcgctg gtagagatgc agctcggggc ccaggtcgac ggccagctgg ttcacgaaca
      721 tccccagacg gccgcgtctg aggatgctca taaggccgga gcaaagcgca ggaatcccct
      781 ggggatcatc aagcaactgc ctaaggcggg cgcgaccgcg tcctgaaggg cacccttgac
      841 ccggtctggg ggtacttcga taattttccg ccctcgccgc ccctcgggcc tcgttcatct
      901 tgcggccggg gaaccagtga gagcgtcacc gggtaactca atgactggac agctcgacat
      961 cgacatcagc ttcaccgagc gcgcggccgc gaaagtgcgg gcactcatcg cggatgagga
     1021 gaatgcggat ctcaaactcc gggtcttcat taccggaggt ggatgcagcg gcttttccta
     1081 cggcttcaca ttcgacgagg aagtagccga agacgatgcc gtcgtcgagc gcgaaggcgt
     1141 tagcatgctc gtcgacagcc tcagctatca gtacctgctg ggcgcggagg tcgactacaa
     1201 agaaggtctg cagggcgcgc agttcgtggt gcagaatccc aacgcgtccg ctacctgcgg
     1261 atgcggcaac tccttcgcca tatgagaagg atccgggtag ccgtcacggc cggtagatcc
     1321 cgccgagaat gcgctctccc gaggcacccg tgacgccggg cgcgttgccc ggaaggcttg
     1381 ccatggtctg gtgagccagc caggcgaaag caccggcttc cagagcatcg ccgtccacgc
     1441 cgtgggcatc ggtcgtctcg atcggggagg gcgtcagctg ggccagcctt tccatgagca
     1501 gccggttgtg gcg
//
LOCUS       DBNHOX010000462         1669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_679146, whole genome shotgun sequence.
ACCESSION   DBNHOX010000462 DBNHOX010000000
VERSION     DBNHOX010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_679146"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /gene="pheA"
                     /locus_tag="AC16UZ_10250"
     CDS             complement(<1..850)
                     /gene="pheA"
                     /locus_tag="AC16UZ_10250"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017906408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIR6247106.1"
                     /translation="MSTRDDRLKAVRDRIDAIDRELQKLLNERAACALEVGEIKQADP
                     DAEAPVFYRPEREAQILSRLKQENQGPLSDDDVARLFREIISCCLNLEQPLTIAYLGP
                     AGTYTEAATVKQFGHFAGTAALASIDEVFREVETGGAHYGVVPVENSTEGMVNHTLDC
                     FIDSPLKICAEVELPIHHAFMVAPDASGEGIEEILSHSQSLAQCRTWLDAHYPGVPRT
                     PVNSNAEAARLVAARTNAAAVAGDMAAERYGLRVIASHIEDQTDNKTRFLVIGQQRVG
                     PSGRDKT"
     gene            complement(847..>1669)
                     /gene="serC"
                     /locus_tag="AC16UZ_10255"
     CDS             complement(847..>1669)
                     /gene="serC"
                     /locus_tag="AC16UZ_10255"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011335372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIR6247107.1"
                     /translation="DRGTADYVNTGSWSTKAIKEAARLCSVNVVASAEEHGFDRIPDP
                     AAWACSADAAYLHICSNETIGGVQFHSFPDRAGGPPLVADMSSDILSRPIDVSRFGLI
                     YAGAQKNVGPAGLTLVVVRKDLVGAARPGTPSMLDYAVHAEAGSMSNTPPTYAWYLAG
                     LVFAWIRSLGGVAALDELNRRKAGKLYAAIDGSNLFRAPVVVEDRSIMNVPFTLPDGE
                     LDAAFLAGAKDRGLLNLKGHRSVGGMRASLYNAVPESAVDALISYMQEFETDHFR"
BASE COUNT          275 a          550 c          552 g          292 t
ORIGIN      
        1 aggtcttgtc acgaccgctc ggacccacgc gctgctggcc gatgaccaga aagcgcgtct
       61 tgttgtccgt ctggtcctcg atgtgggagg cgatgacccg cagtccgtaa cgttcggccg
      121 ccatgtcgcc cgcaacggcg gcagcattgg tacgggcggc aaccagcctc gctgcctcgg
      181 cattgctgtt gacgggcgtg cggggaacgc cgggatagtg cgcgtccagc caggtgcggc
      241 actgagcgag cgactgggag tgggacagaa tctcctcgat gccctcgccg ctggcgtccg
      301 gcgccaccat gaaggcgtga tggatgggca gctccacctc cgcgcagatt ttcagcggcg
      361 agtcgatgaa gcagtcgagc gtgtggttca ccatgccttc cgtggagttc tccaccggca
      421 ccacgccgta atgggcgccg ccagtttcca cctcgcgaaa cacttcgtcg atggatgcga
      481 gcgccgccgt tcccgcaaag tgcccgaact gcttgacggt ggcggcttcg gtgtaggttc
      541 ccgccggccc gaggtaagcg atggtcagag gctgctcgag attcagacag caggaaatga
      601 tctcgcggaa aaggcgcgcg acgtcgtcgt cgctcagcgg cccctggttc tcctgcttga
      661 gccgcgaaag gatctgggct tcccgctccg gccgatagaa caccggcgcc tccgcatccg
      721 ggtccgcctg cttgatctca cccacctcca gcgcgcaggc agcccgctcg ttcaggagtt
      781 tctggagctc ccggtcgatg gcgtcgatac ggtcgcggac cgctttgagc ctgtcgtccc
      841 gggtgctcat ctgaagtgat cggtctcgaa ttcctgcatg tacgagatca gcgcatccac
      901 cgcggattcg ggcaccgcgt tgtaaaggct cgcccgcatc ccgcccacgc ttcggtgtcc
      961 cttcagattg agcaggccgc ggtccttggc gccggccagg aatgcagcat ccagctcgcc
     1021 gtcggggagc gtgaacggca cgttcatgat ggatcggtct tcaaccacca ccggcgcgcg
     1081 gaacagattg ctgccgtcga tggccgcgta gagcttgccc gccttgcggc ggttgagctc
     1141 atcgagggcg gcgaccccgc ccagggacct gatccaggcg aacaccagcc ccgccaggta
     1201 ccaggcgtag gtcggcgggg tgtttgacat ggaccccgcc tcggcgtgaa cggcgtagtc
     1261 cagcatgctg ggcgtgccgg gccgcgcggc cccaaccaga tccttgcgca cgacgacgag
     1321 cgtcagtccc gccggcccga cgttcttctg ggcgccggcg tagatgagac cgaaacggct
     1381 gacgtcgatg ggccgggaca ggatgtccga cgacatgtcc gcgaccagcg gcgggccgcc
     1441 ggcgcggtcg ggaaagctgt ggaactgaac ccctccgatg gtctcgttcg agcatatatg
     1501 gaggtaggcg gcatcagcgc tgcaggccca cgcagcagga tccggaatcc ggtcgaaacc
     1561 gtgctcctcg gcactcgcaa ccacgttgac cgagcacagg cgggcggctt ccttgatcgc
     1621 cttggtcgac caggagcccg tgttgacgta atccgccgtg ccacgatcg
//
LOCUS       DBNHOX010000463         2553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_550726, whole genome shotgun sequence.
ACCESSION   DBNHOX010000463 DBNHOX010000000
VERSION     DBNHOX010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_550726"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="AC16UZ_10260"
     CDS             <1..449
                     /locus_tag="AC16UZ_10260"
                     /inference="COORDINATES: protein motif:HMM:NF016121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR6247108.1"
                     /translation="GYDHWAGTTDDARLVPVYVSRRDGAATRLVLPVHGRGMWSTIYG
                     YVALDADLTTVAALVIHRHGETPGVGDRIQDPAWRAKWRGKRAFGPDGAVAIHVARGG
                     NGQQPGRVDTITGATVTSEAVGQLVAGWLAIYSPWLSRIAAPGARI"
     gene            466..1116
                     /locus_tag="AC16UZ_10265"
     CDS             466..1116
                     /locus_tag="AC16UZ_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HIR6247109.1"
                     /translation="MLRHLTHPLVSENPITVQILGLCSALAVSSTLKPAFIMSVSVVA
                     VLVFSNVSVSLLRNVFPRSIRLILEVTLIASAVIVVDEVLKAFAPETADILTVFVGLI
                     VTNCIILGRAEAFAMHHRVAPSLADALGNGLGYALVLLAVATARELFGHGTLLDVQIL
                     PLSEAEGGWYRPNQMMRFAPSAFFLIGLIIWGVRSVRAEQVEAGAYPPASPSYREG"
     gene            1116..1724
                     /gene="nqrE"
                     /locus_tag="AC16UZ_10270"
     CDS             1116..1724
                     /gene="nqrE"
                     /locus_tag="AC16UZ_10270"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HIR6247110.1"
                     /translation="MEHLLNIVVRAIFVENLALVFLLGMCTYIAVSKRVETAIGLGVA
                     VVVVQLITVPVNNLLYEYVLTPGALAWMGLGTLDLSFLKLITFIGVIAALVQMLELVI
                     ARFAPQLQQHLGIYLPLLTVNCAILGASLLMVQRSYDFAESVAYGLGVGIGWAMAIVL
                     FAAIRERLKYSDVPKGLEGLGLAFIVTGFLAFGFIGFTGVEF"
     gene            1727..>2553
                     /locus_tag="AC16UZ_10275"
     CDS             1727..>2553
                     /locus_tag="AC16UZ_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6247111.1"
                     /translation="MLEIVVPAVALTTIVFLLSLAVLLVRRGLVPGEDVEILINDQRA
                     LRGDAGEKLLWALADHDVYLPAACGGRGSCGQCRIEVTRGGGAITPAEENHIARRDAA
                     RGVRLACMVTLGDDVAVHLPPELLEIRRWSSKVLANRHVTTYLKELTLELPPGLELNF
                     NAGAYVQLEAPAGIVRFDGFDIAAEYDAEWQRHGLRDLVVEIPETTTRAYSLASHPGE
                     SGAIKLVVRIALPPPDAPVGTPPGRVSSYAFGLKPGDEVWLAGPFGEFRARDTDREMV
                     "
BASE COUNT          375 a          828 c          835 g          515 t
ORIGIN      
        1 acggctatga ccactgggcc ggcacgacgg acgacgcgcg gctcgttccc gtatacgtct
       61 cgaggagaga cggcgcagcg acccggctgg tacttccggt tcatggtcga gggatgtggt
      121 cgaccatata cggctacgtg gccctggatg cggatctgac gaccgtcgct gctctggtca
      181 tccatcgtca tggtgagacg ccgggcgtgg gcgacaggat tcaggatccc gcctggcggg
      241 cgaagtggcg tggcaaacga gcattcgggc ccgatggcgc ggtcgcgatt cacgtcgccc
      301 gcgggggcaa tgggcagcag ccgggcaggg tggatacgat cactggcgcg accgtgacga
      361 gcgaagccgt gggccagctg gtcgccggct ggttggccat ctactcgccc tggctttccc
      421 ggatcgcggc gcctggagca cgaatatgaa gcgtggacac cgttcatgtt gaggcatctc
      481 acccatccgc tcgtcagcga gaacccgatc accgtgcaga tcctcggtct ctgctccgcc
      541 cttgcggtgt ccagcaccct caagccggct ttcatcatga gcgtttccgt tgtcgcggtg
      601 ctggttttct cgaatgtgtc cgtcagcctg ttgagaaacg tctttccccg cagcattcgt
      661 ctgatcctgg aagtgacgct catcgcctcg gcggtgatcg tggtggacga ggtgctcaag
      721 gccttcgctc cggagacggc tgacatactg accgtgttcg ttggcctgat tgtcaccaac
      781 tgcatcattc tcggacgggc cgaagcgttt gccatgcacc accgggtggc ccccagtctt
      841 gccgacgccc ttgggaatgg tctgggctac gcgctggtgt tgctggccgt cgccacggct
      901 cgcgagctgt tcgggcacgg gacgttgctc gacgttcaga tactgcccct gagcgaggcg
      961 gaaggtggtt ggtatcgccc caaccagatg atgcgctttg cgcccagcgc tttttttctg
     1021 atcgggctca tcatctgggg cgttcgcagc gtgcgcgcgg aacaggtcga agccggagct
     1081 tacccgccgg cgagcccgag ctaccgggag ggctgatgga gcatctcctg aacatcgttg
     1141 tgcgcgccat cttcgtcgag aacctggctt tggtgttcct gctcggcatg tgcacgtaca
     1201 tcgcggtctc caagcgtgtg gagaccgcca tcgggctcgg cgtcgccgtc gtcgtcgtgc
     1261 agctcatcac cgtgcccgtc aacaacctcc tctacgaata cgtcctcact cccggggcgc
     1321 tggcctggat ggggctggga accctggatc tttcgttcct caagctgatc accttcatcg
     1381 gcgtcatcgc cgctcttgtc cagatgctgg agctcgtcat cgcgcggttc gcgccgcagc
     1441 tgcagcagca cctgggcatc tacctgccgc tgctcaccgt caactgcgcc atactcggcg
     1501 cttccctgct catggtgcag cgcagctatg acttcgccga aagcgtcgct tacgggctcg
     1561 gcgtcggcat cggctgggcg atggccatcg ttctctttgc cgccatccga gagcgcttga
     1621 agtacagcga tgtgccgaag gggctcgaag gccttggcct ggccttcatc gtgaccgggt
     1681 tcctcgcctt cggcttcatc ggcttcaccg gagtggagtt ctagacgtgc tggagatcgt
     1741 cgtgccagcc gtcgcgctga cgaccatcgt cttcctgctc tcgctggcgg tgctcctcgt
     1801 ccgccgcggc ctggtgcccg gcgaggacgt ggagattctg atcaacgatc agcgggctct
     1861 gcggggcgac gccggcgaga agctcctgtg ggcgctggcc gaccacgatg tctatcttcc
     1921 ggcggcctgc ggtggccggg gcagctgcgg gcaatgtcgc atcgaggtga cgcggggcgg
     1981 tggcgccatc acgccggcgg aagagaacca cattgcgcgc cgtgatgccg ctcgcggcgt
     2041 gcgcctcgcc tgcatggtca cccttggcga cgacgtcgcc gtgcatctgc ctcccgagtt
     2101 gctggagatc cgccgctgga gctcgaaggt tctggcaaac cgtcacgtga ccacctactt
     2161 gaaggagctc accctcgagc tgccgcctgg cctggagttg aatttcaacg ccggcgccta
     2221 cgtgcagctc gaggccccgg ccggcatcgt gcggttcgac ggcttcgaca tcgccgccga
     2281 gtacgacgcc gaatggcaac ggcacggcct tcgggatctc gtcgtggaaa ttccggagac
     2341 gaccacccgc gcgtattctc ttgcaagcca ccccggcgaa tccggcgcca tcaagctggt
     2401 cgttcgcatc gccctgccgc ccccggatgc cccggtgggg accccgcccg ggcgggtctc
     2461 gtcctacgcg tttggcctga agcccggtga cgaagtctgg ttggcgggcc cgttcggcga
     2521 gtttcgcgcg cgggataccg accgggaaat ggt
//
LOCUS       DBNHOX010000464         1552 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_679319, whole genome shotgun sequence.
ACCESSION   DBNHOX010000464 DBNHOX010000000
VERSION     DBNHOX010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_679319"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="AC16UZ_10280"
     CDS             <1..1194
                     /locus_tag="AC16UZ_10280"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6247112.1"
                     /translation="PALFEANAMLPYFERLRAEAPVHYCEHSQFGPYWSITRFEDVKY
                     VDTHEKLFSSDIRNGGIRLGQQPDPEPDPMFHLPMFIMEDPPKHDEQRKVIAPMFTAR
                     HLAGLADLIRERAGRILDGLPVGETFNWVREVSVELTGQMLATLFDVPQEDRHKLIHW
                     SDTVERIGDPDYFETPEQGFQELWKCWEYFDAVWKERQAQSEPGSDLISMLVHGESTK
                     DMPPNEYLGNILLLIVGGNDTTRNSITGGVLALNQNPAEYEKLLADPGLIPNMVPEII
                     RWQSPVAHMCRTALEDTEVGGRTIRKGDKVAMWYLSGNRDEDVIDDANRFIIDRANAR
                     QHLSFGFGIHRCVGNRLGEMQLGIIWEEILKRFARVEVVGEPQYLRSSFIRGIRELPV
                     RVHPA"
     gene            complement(1199..>1552)
                     /locus_tag="AC16UZ_10285"
     CDS             complement(1199..>1552)
                     /locus_tag="AC16UZ_10285"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6247113.1"
                     /translation="AARIGFGLLRTLVAAPARGQPPDLERARKAYHEDLPPIFTYLED
                     VIGDRPFLVGDRFSIADIALASQLAGMRVTRVPIDGGRWPNLADYADRLLARPSFVDR
                     LAEVASRMPAEDFVP"
BASE COUNT          294 a          518 c          493 g          247 t
ORIGIN      
        1 ccggccctgt tcgaggccaa cgccatgctg ccctacttcg agcggttgcg cgcggaagcg
       61 cccgtgcatt actgcgagca cagtcagttc ggcccctact ggtccatcac ccggtttgag
      121 gacgtgaagt acgtggatac ccacgagaaa ctcttctcgt cggacatacg caacggcggg
      181 atccgcctgg gccagcagcc ggacccggag ccggacccca tgttccatct gcccatgttc
      241 atcatggagg atccgccgaa gcacgacgag cagcgcaaag tgatagcacc catgttcacg
      301 gcgcggcatc tggccgggct ggcggatctg atccgtgagc gtgccggccg catcctggac
      361 gggctgccgg tgggggagac cttcaactgg gtccgcgaag tttccgtgga actcaccggg
      421 cagatgctcg caaccctctt cgacgtcccc caggaggatc gccacaagct catacactgg
      481 tcagacaccg tcgagcgcat cggcgatccg gactacttcg agacccccga gcagggcttt
      541 caggagctgt ggaagtgctg ggagtacttc gatgccgtct ggaaggagcg ccaggcccag
      601 agcgagccgg gcagcgatct gatctccatg ctcgttcacg gggaatccac gaaggacatg
      661 ccacccaacg agtacctggg caacatcctg ctgctcatcg tcggcggcaa cgacaccacc
      721 cgcaactcca tcaccggcgg cgtgcttgcg ctgaaccaga acccggccga gtacgagaag
      781 ctgctcgccg atcccgggct gataccgaac atggtcccgg agatcatccg ctggcagtcg
      841 ccagtggccc acatgtgccg gacggctctg gaagacacgg aagtcggcgg caggaccatc
      901 cgcaagggcg acaaggtggc catgtggtac ctctccggta accgggacga agatgtcatc
      961 gacgatgcca atcgcttcat catcgaccgg gccaacgcgc gtcagcactt gtccttcggc
     1021 ttcggcatcc accgctgcgt gggcaaccgg ctcggcgaga tgcagctggg catcatctgg
     1081 gaggagatac tcaagcgctt cgccagggta gaggtggtag gcgagcctca gtatctccgc
     1141 tcgagcttca tccgcggcat tcgcgagctg cccgtgcgcg tgcacccggc ctgatcgctc
     1201 agggcacgaa gtcctccgcg ggcatacgcg aagcaacctc tgccagccgg tctacgaagc
     1261 tcggccgggc gagcagacgg tcggcgtagt cggccagatt gggccagcga ccaccgtcga
     1321 tgggcacccg cgttaccctc atgcccgcca gctgcgaagc cagggcgatg tcggcaatag
     1381 agaagcggtc gcccaccagg aacggccgat cgccgatgac gtcttcgaga taggtgaaga
     1441 tcggcggcag gtcctcgtgg taggccttgc gcgcccgctc gagatccggc ggctgccccc
     1501 ttgccggggc cgccaccagc gtgcgcagca gcccgaaccc gatgcgcgcc gc
//
LOCUS       DBNHOX010000465         7423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1193487, whole genome shotgun sequence.
ACCESSION   DBNHOX010000465 DBNHOX010000000
VERSION     DBNHOX010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7423
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1193487"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="AC16UZ_10290"
     CDS             complement(<1..453)
                     /locus_tag="AC16UZ_10290"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR6247114.1"
                     /translation="MANDFANRLNPPTTRRPKARPPSATRRKERRFSGSSFASGLLIG
                     GACMAVALNLPDPQPERPPAAATAASAPPGSVTFEFDKDLRDAFVEADPGQYETDAGH
                     DGSRPVSYLIQAASFPVAADADSLQAELAQMGLAAQSVPARLNAGTWFR"
     gene            complement(531..2702)
                     /locus_tag="AC16UZ_10295"
     CDS             complement(531..2702)
                     /locus_tag="AC16UZ_10295"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004067.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIR6247115.1"
                     /translation="MNPLTQTNVDVVRVAVAKPLHQLFDYAVPPDMTPPVAGARVVVP
                     FGRQRLTAICVERNPDDAHAQLKPLLSVLDDTAAIPEELRALAGWLCDYYQHPLGEVL
                     ATMLPAEARRGHELTIVRPEQWTATGTRSDLARAPRQQALMEALAAAGGTLSASALAE
                     AGFSRALIRAAAGKGLITRTEAATGFTLGIDEQPPELSDEQAGVLSDLLARTDCFAPS
                     LLEGITGSGKTEIYLQLIDNVRKAGHQVLVLVPEIALTPQTVGRFERRFGSGGGVASL
                     HSNLGDQERLQTWLKCRSGEVSVLIGTRSAVFAPFAKLGLIVVDEEHDGSFKQQDGLR
                     YSARDVAVKRAQTLDIPLLLGSATPSLESLNNAMAGRYAHLRLRHRAGGARPPRFHLL
                     DIRGQSLDDGLSQDLLRIVGQHLNRGGQALVFLNRRGYAPTYLCPRCSWQAACGHCEM
                     RLTLHLSPRALLCHHCGHQRPLPSACPDCGHAELIAVGAGTQRTEAGLAKRFPGIPLY
                     RVDRDTTRSQRRMEAQFEAIHKGDPAILVGTQMLAKGHHFPKVTVVAVVNADGGFLSA
                     DYRAPERTAQLITQVAGRAGRAEDPGEVWIQTYQPDGVLLRSLIDEGYDGFARRELAI
                     RKSSGLPPFRPMALLRAESRDAESARNFLTSVRDRLGSPLEVLGPAPAPIARVANRLR
                     FQLMVMADSRRELHAGIRPIRDLTAPGTIRWSVDIDPYDTF"
     gene            2822..3037
                     /gene="rpmE"
                     /locus_tag="AC16UZ_10300"
     CDS             2822..3037
                     /gene="rpmE"
                     /locus_tag="AC16UZ_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR6247116.1"
                     /translation="MKADIHPEYNQIKATCSCGNVIETCSTLEGDIHLDVCSACHPFY
                     TGKQKILDAGGRVERFRKRFGNRSVSS"
     gene            3202..4686
                     /locus_tag="AC16UZ_10305"
     CDS             3202..4686
                     /locus_tag="AC16UZ_10305"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR6247117.1"
                     /translation="MRRRLLRSGYPAPVRQSLAQVGRLIARRRPQLRLQLCALLLPGL
                     VLSGMAVAGPGEKVYKEMLANDQIYQDQEWQAYVNQIGRRLLAVSPDAGKDYHFYILD
                     NPTVNASAFPDAYIFVNRGLIAYLRSEDELAGVIGHEIGHVVGRHARRSNMMGFFGNV
                     AGFIGSIMTGTGAIADMGNTVTQTLRSGYGREFELEADEYGGEFLARAGYNPLAMIDV
                     IHVLKDHSLFAKNVLGQPAVYHGLFSTHPKNDKRLHDAVQKSQHIFPDELREPERDFW
                     EMIDGMVYGDEAATGLIKGETYYHGGLRVVVQFPQGWDVTNTASEVLGRAPGGTPDAF
                     ITVQRLNPAESEQTPEEYVKETLKRDDVTDGESLEISGYSAFIGTVEVASGNAQARRI
                     AVIYKDNGVYLFKGEVGPVGDVNAFEPQWRATLESFRAMTAADLMVANSQRLQVVVAN
                     PGDSYDKLARKVSIKSYPAETLRVLNGDHPIGEPRAGDYIKIVQ"
     gene            complement(4702..7119)
                     /locus_tag="AC16UZ_10310"
     CDS             complement(4702..7119)
                     /locus_tag="AC16UZ_10310"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR6247118.1"
                     /translation="MLSLAGAFLYLDPQIPRAETYRQVKLETPLRVYSSDEKLIAEFG
                     ERRVIPLKLEEAPPLFVAALLDTEDKRFYDHSGIDYISLLNDTVELALNREIRSGAST
                     ITMQLARNISFSLEQTFIRKFKEMLLALQIEQELGKDEILELYMNVVPFGKRAYGAQA
                     AALTYYGKRLDELNLAQLAMLAGIPQAPTAGNPINGPARAVRRRNVVLRRMLDQGSIS
                     QAAFDEAIAAPITASLHDRALDVGAPFPAEWVRAELINRYSDLYTGGYQVYTTIVADL
                     QQTATRSLRAGRKSYDRRHGYRGPEQSLGALTEEEYLQALREARSYADLDPAVIVSVD
                     EQGADALLGDGSRGRIAMAEMSWARRYLDVDRRGPPPERPSDVVQVGDVVRVEAIAEP
                     AEAGEAESAGDDPEAEPAPAWRLAQLPDIQGALVSMDPRTGAVLALEGGLDFAMNQYN
                     HALQAARQPGSGFKPFVYSAALDRGVTPASIFMDAPLVFDDQNLESQYRPDNDNNRYN
                     GPTRLREALYRSINLVSMRVLLEVGAGSVMDHVRKFGFDTSTFPRNLQLAIGGGTMAV
                     TPLQMATAYAVFANGGWQIEPHIVDRVVALDGETVYQASHPRVCDPCDQDPATDLDSE
                     EPANLEDLLAEADDAPIKAERVVDERNTYIMNDMLKDVIRVGTGRRARTLERSDIAGK
                     TGTTNDAADTWFNGYSPDVVTTVWVGFPDHRPLGATEYGSNTPLPIWIEFMSQALANK
                     PERVPAQPAGVVTMKIDRDSGELAGSQQTDAIFELFLAEHTPQPPPSAAAGPSEPGTD
                     LKPMDIF"
     gene            7298..>7423
                     /locus_tag="AC16UZ_10315"
     CDS             7298..>7423
                     /locus_tag="AC16UZ_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HIR6247119.1"
                     /translation="MFGLFKNKSNVLLGLDISSTSVKLLELSKSNDRFRVESYAVE"
BASE COUNT         1279 a         2348 c         2485 g         1311 t
ORIGIN      
        1 ccggaaccag gtgccggcgt taagccgcgc cggaaccgac tgggccgcca ggcccatttg
       61 cgcaagctct gcctgaaggg agtcggcatc ggcggctacc ggaaacgacg ccgcctggat
      121 gagatagctg accggtcggg atccgtcgtg gccggcgtcc gtctcgtact gcccagggtc
      181 cgcctccaca aaggcgtcgc gcaaatcctt atcgaactcg aaggtcacgg atccgggcgg
      241 cgcgcttgcc gcggtagctg cggcgggcgg gcgttcgggc tgcgggtcag ggaggttcaa
      301 agccacggcc atgcacgcgc cgccgatcag cagaccggaa gcgaagctcg aaccgctgaa
      361 acgcctctcc ttgcgacgtg tcgcggaggg cggccgggcc ttggggcgcc gggtcgtcgg
      421 cggattgagt cggtttgcaa aatcgttggc catgggctgc gaatgatagg ctcgcggcgg
      481 gtcgtccgcc acgatgtccg gtgaacgccc gttggggcgg ccggtaaagg tcagaacgta
      541 tcgtagggat cgatgtccac agaccagcgg atggtgcctg gcgcggtgag gtctcgtatc
      601 ggccggatgc cagcatgcag ctcgcggcgc gagtctgcca tcaccatgag ctgaaaccgg
      661 aggcgattgg ctactcgggc gatgggcgct ggagccgggc cgagcacttc taaggggctt
      721 cccaggcggt cgcggacgct ggtcaaaaaa tttcgggcgc tctcggcgtc ccggctctcc
      781 gcccgcaaca acgccatggg ccggaagggc ggcaggccgc tggacttgcg aatggccagc
      841 tcccgccggg cgaacccgtc gtagccctca tcgatgagcg agcgcagcag cacgccgtcc
      901 ggctgatagg tctggatcca cacctcgccg ggatcctcgg cacgacccgc gcgcccggcg
      961 acctgggtga tgagctgggc cgtgcgctcg ggtgcgcggt agtcggcgct gaggaacccg
     1021 ccgtcggcgt tgaccacggc caccacggta accttcggaa agtggtgccc tttggccagc
     1081 atctgcgtgc cgacgaggat tgccggatcg cccttgtgga tggcctcgaa ctgggcctcc
     1141 atgcgacgct ggcttcgggt ggtatcgcgg tcgacacgat acagaggaat ccctgggaac
     1201 cgcttagcca gcccggcctc ggtgcgctga gtaccggcac cgacggcaat cagctccgcg
     1261 tgcccgcagt ccgggcaggc gctcggcagc ggtcgctgat ggccgcagtg gtggcagagc
     1321 agcgcccgcg gcgacaggtg cagcgtcagt cgcatctcgc agtgcccgca ggcagcctgc
     1381 cagctgcagc ggggacacag gtaggtgggc gcgtacccgc gtcggttcag aaagaccagg
     1441 gcctgcccgc cccggttgag atgctggccc acgatgcgca gcagatcctg gctgaggccg
     1501 tcatcgaggg attgaccgcg gatgtcgagc aggtggaacc ggggcggccg ggcgcccccc
     1561 gcccggtggc gcagccgaag gtgggcgtag cggccggcca tggcgttgtt gaggctttcc
     1621 agtgaaggcg tggcgctccc gagcagcagc ggtatgtcca atgtctgcgc acgtttcacc
     1681 gccacgtcac gagccgagta cctcagcccg tcctgctgct tgaaggagcc gtcgtgctcc
     1741 tcgtccacca cgatcaggcc cagcttcgcg aacggcgcga acacggcgga gcgcgtgccg
     1801 atgagcacgc tgacctcgcc gctgcggcac ttcaaccagg tctgtaacct ctcctgatca
     1861 cccaggttgg aatggaggct ggccaccccg ccgccggatc cgaagcggcg ttcgaagcgc
     1921 ccgacggtct gaggggtgag cgcgatctcg gggacgagca cgagaacctg gtggccagct
     1981 ttccgcacgt tatcgatcag ttgcaggtag atttcggtct tgccgctgcc cgttatcccc
     2041 tccagaagtg atggcgcaaa gcaatcggtg cgagcgagca ggtcggagag cacgccggcc
     2101 tgctcgtcgc tcagctccgg cggctgctcg tcgatgccca gggtaaagcc cgtggcggcc
     2161 tcggttcggg tgatcagccc cttgcctgcg gcagcgcgga tgagggcccg ggaaaaaccc
     2221 gcttcggcga gcgccgacgc ggacagggtg ccgcccgcgg cagcgagggc ttccatgagc
     2281 gcctgctggc ggggcgctcg cgccaggtcg gacctggtgc cggtcgccgt ccattgctcc
     2341 ggcctgacga tcgtcagctc gtgcccgcgg cgagcctctg ccggcagcat ggtggccagc
     2401 acctcgccga gagggtgctg gtagtagtcg caaagccacc cggcgagggc gcgcagctcc
     2461 tcgggaatgg cggcggtatc gtccaggacg ctgagcagag gcttgagctg ggcgtgggcg
     2521 tcgtcggggt tgcgctccac gcagatcgcg gtaagacgct gcctgccgaa cggcacgacg
     2581 acacgcgccc ccgcaaccgg tggcgtcatg tccggcggca ccgcgtagtc gaacagctgg
     2641 tgaagaggct ttgccacggc cacgcgcacc acgtccacgt tcgtctgggt caacgggttc
     2701 acagccctct cctcgggagc tggcattgta atgcttcgac cggcgcgcgg gttgccattc
     2761 accgcgcctc ttaataaact tcgcgccctt ttcccggaac gtgatccaag ggagcagtca
     2821 ggtgaaagcg gatatccatc ccgaatacaa ccaaatcaaa gccacttgca gctgtggaaa
     2881 cgtcatagag acctgctcga cgctggaagg tgacatccac ctggacgttt gctcggcttg
     2941 ccatccgttc tacaccggca agcagaagat cctggacgcc ggcggtcgcg tcgagcgatt
     3001 ccgcaagcgc ttcggcaacc gctccgtttc ttcctgaccg cacccctcac ggcaacccct
     3061 cagcgctgac gccgccccgt ggatcgcgga cggcgttgaa ccgtttcatg ctcctccagt
     3121 ctaaggttcg gtgtccctgg cggttttcgc agcgtctcgt aagatacggc tgacctgctc
     3181 gtgattcctg gatcccggcg aatgagaaga cgtctactcc gaagcggcta cccggcgcct
     3241 gtgcggcaat cgctggcgca ggtgggtcgg ctgatcgcgc gacggcgccc gcagctgcgc
     3301 ctgcagctct gcgcgctgct tctgcccggc ttggtcctct ccggcatggc ggtggctggc
     3361 ccgggagaga aggtctacaa ggagatgctg gccaacgatc agatctacca ggaccaagag
     3421 tggcaggcct acgtgaacca gattggccgg cgcctgcttg cggtgagccc tgacgcgggc
     3481 aaggattacc acttctatat cctcgataat cccacggtga acgcctcggc ttttcccgac
     3541 gcgtacatat tcgtgaaccg cgggctcatc gcctacctca ggtctgagga cgagctggcc
     3601 ggggtcatcg ggcacgagat cggccacgtc gtcggccgcc acgcccgccg cagcaacatg
     3661 atgggtttct tcggaaacgt tgccgggttc atcggatcga tcatgaccgg aaccggtgcc
     3721 atcgcagaca tgggcaacac ggtgacccag acgctgcgct ccggctatgg ccgcgagttc
     3781 gagctcgaag ctgacgagta cggaggagag tttctggccc gtgccggtta caacccgtta
     3841 gccatgatcg acgtgatcca cgtcctcaag gaccactcgc tgttcgccaa gaacgtgctc
     3901 ggccagccgg ccgtctatca cggtttgttc tcgacgcacc cgaagaacga caagcggctg
     3961 cacgacgccg tgcagaagtc tcagcacatc tttcccgacg agctgcgcga gccggagaga
     4021 gatttctggg agatgatcga cggcatggtc tacggggacg aggcggcgac ggggttgata
     4081 aagggcgaga cctactatca cggcgggttg cgggttgtcg ttcagttccc gcagggatgg
     4141 gacgtcacga acacggcctc ggaagttctc ggcagagctc ccggcgggac gcctgatgcc
     4201 ttcatcaccg tccagcgcct gaaccctgcc gaatccgagc agaccccgga ggagtacgtc
     4261 aaagaaaccc tcaaacggga cgacgtcacc gacggcgaga gcctcgagat cagcggctac
     4321 tcggccttca tcggcaccgt ggaggtggcc tcgggcaacg cgcaggcgag acgcatcgcg
     4381 gtgatctaca aggacaacgg ggtatatctc ttcaagggag aggtgggccc ggttggcgac
     4441 gtcaacgcct tcgagcccca atggcgggcg acgctggagt cgtttcgcgc gatgaccgcg
     4501 gcagatctga tggtggccaa cagccagcgt ctgcaggtcg tggtcgccaa cccgggtgac
     4561 agctacgaca agctcgcccg caaggtgtcc attaaatcct atcccgcaga aaccctgcgc
     4621 gtgttgaacg gcgaccaccc catcggggag ccaagggccg gcgactacat caagatcgtt
     4681 cagtagccct gcaggctcgg ctcagaagat gtccatcggc ttgaggtcag tgccgggttc
     4741 gctgggtccg gcagccgccg atggcggtgg ctggggcgtg tgctccgcaa gaaacagctc
     4801 gaatatcgca tccgtctgct gggagccggc gagctctccg ctgtccctgt cgattttcat
     4861 cgtgacgacc ccggccggct gagcgggaac ccgttccggc ttgttggcca gtgcctggga
     4921 catgaactcg atccagatcg gcaggggcgt gttggatccg tactccgttg cgcccagcgg
     4981 ccggtggtcg gggaaaccca cccacacggt cgtgacgacg tcggggctgt aaccgttgaa
     5041 ccaggtgtcg gcggcgtcgt tggtggtgcc ggtcttgcct gcgatgtcgc ttcgctcgag
     5101 agtacgcgcc cggcgaccgg tcccgacccg gatcacgtcc ttgagcatgt cgttcatgat
     5161 gtaggtattc cgctcgtcga ctacccgctc ggccttaatt ggcgcatcgt cggcctcggc
     5221 gagaagatcc tcgaggttcg cgggctcctc gctgtcgagg tccgtagcgg gatcctgatc
     5281 gcagggatcg caaaccctcg ggtggctggc ctggtagacg gtttcaccat ccagcgcgac
     5341 cacccgatcg acgatgtgcg gctcgatctg ccagccaccg ttggcaaaga cagcataggc
     5401 cgtggccatc tgcagcggcg tgaccgccat ggttccgccg ccgatggcaa gctgaaggtt
     5461 gcgcggaaac gtgctcgtgt cgaagccgaa tttgcgcacg tgatccatga cgctgccggc
     5521 gccgacctcg agcagcacgc gcattgagac caggttgatg gatcggtaga gggcttcccg
     5581 gagcctggtc gggccgttgt agcgattgtt gtcgttgtcg ggccggtact gcgactccag
     5641 attctgatcg tcgaacacta agggcgcgtc catgaatatg ctcgccggcg tgaccccgcg
     5701 gtcgagcgcg gccgagtaga cgaagggctt gaatccggag cccggctgcc gggcagcctg
     5761 aagcgcgtga ttgtactgat tcatcgcgaa gtccaggccg ccttccagag ctaaaacagc
     5821 gccggtacgg gggtccatgg acaccagggc gccctgaatg tcaggcagct gggccagccg
     5881 ccaagccggc gccggttcgg cttcgggatc gtctccggca ctttccgcct ctccagcctc
     5941 ggccggttcg gcaatagctt ccacccgcac cacgtcgccc acctggacca cgtccgaggg
     6001 ccgctcgggc ggggggccgc ggcgatccac gtccaggtag cgtcgcgccc atgacatctc
     6061 ggccatggcg atgcggccgc ggctgccgtc gccgagcagg gcgtccgcgc cctgctcgtc
     6121 cacggagacg atgacggccg gatcgaggtc ggcgtaggat ctcgcctcgc gcagcgcttg
     6181 gaggtattcc tcttcggtga gcgcgcccag tgactgctca gggcctcggt agccgtggcg
     6241 cctgtcgtag ctcttccggc cagcgcgcag ggaacgggtg gccgtctgct gcagatcggc
     6301 cacgatggtg gtgtagacct ggtacccgcc cgtatatagg tcgctgtagc gattgatcag
     6361 ctccgcgcgg acccactccg ccgggaacgg cgcgcccacg tccagggcgc gatcgtggag
     6421 gctggccgtg atcggggcgg cgattgcttc gtcgaaagct gcctgactta tcgagccctg
     6481 atcgagcata cgccgcagca ccacgttgcg acggcgcacc gcgcgcgccg gcccgttgat
     6541 gggattaccc gccgttgggg cctggggaat gcctgccagc atggcgagct gggcgagatt
     6601 cagctcgtcc agacgcttgc cgtagtaggt cagggcggcc gcctgcgcgc cgtaggcccg
     6661 cttgccgaac ggcaccacgt tcatatacag ctcgaggatc tcgtccttgc cgagctcctg
     6721 ctcgatctgc agcgcgagca gcatctcttt gaatttgcgg atgaacgtct gctccaggga
     6781 gaaggagatg ttgcgcgcga gctgcatggt gatggtgctt gcgcccgatc ggatctcccg
     6841 gttcagcgcg agctccacgg tgtcgttgag cagcgagatg tagtcgatgc cgctgtggtc
     6901 gtaaaaacgc ttgtcttcgg tgtccagaag ggcggcgacg aacagcggcg gcgcttcctc
     6961 cagcttcagg ggtatgaccc gccgctcgcc gaattccgca attagctttt cgtcgctcga
     7021 gtagacgcgc agcggagtct cgagcttgac ctgccgatag gtctccgcgc ggggtatctg
     7081 cggatccaaa tagagaaatg cgcctgcaag actgagcact gcggcacata aaccggcgat
     7141 gccgagccac ataatacggg tcatgacgcg tcgggaccgc ggtctcatga tggcggcata
     7201 gttacaggga agcgattgct ctcgtagcaa tcctggtatt gatcaaaaca ttgcatgaca
     7261 aggacgtaag tgcccgcgct gctaaggaaa aattaccgtg tttggcctct ttaagaacaa
     7321 gtccaacgtt ctgcttgggc tggatatcag ctccacttcc gtcaagttgc tagagctgtc
     7381 taaatcaaac gaccggtttc gcgtggaatc ctacgcggta gaa
//
LOCUS       DBNHOX010000466         1514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1643845, whole genome shotgun sequence.
ACCESSION   DBNHOX010000466 DBNHOX010000000
VERSION     DBNHOX010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1643845"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="AC16UZ_10320"
     CDS             <1..727
                     /locus_tag="AC16UZ_10320"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6247120.1"
                     /translation="TQGLDGKNREAGFRIAGLYDAEGTALEKQFVFTGVAALQQMVDA
                     DVVTEVSVKLEDEALVAHRSLERDVKSRLTSFFSTLDVMDWQQLEPQAAAMFVYVDSA
                     ILIWFMVIMGALIFGLVNTLITAVMERIREFGMLRAVGMKPSAVVMQVVLESTFIMLA
                     GVAVGIFVGWLLCTVWLGDGIDLSQWAEGIEMAGMRSRLTPKLMGQDVVLVTVLSLLF
                     GVIAALYPAWRAVKIKPLEAIRR"
     gene            724..1422
                     /locus_tag="AC16UZ_10325"
     CDS             724..1422
                     /locus_tag="AC16UZ_10325"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6247121.1"
                     /translation="MNDLVVRCQSISRTYQDDTVPVRALVDVDFEVARGEFVSLAGPS
                     GSGKSTLLNIIGGLDRPDSGTVEVDGVLLNELSESDLSDLRLRKLGFVFQAYNLIPVL
                     SARENVEFIMQLQGVPAGERRSRAEAMLEELGLEGMTDRRPGEMSGGQQQRVAVARAI
                     VTHPVLLLADEPSANLDSHTTEDLLKLLRRLNEEHGVTIVTATHDPLVMSYARRQVRL
                     RDGQIIEDSAREAA"
BASE COUNT          252 a          442 c          526 g          294 t
ORIGIN      
        1 tactcagggg ctggacggaa aaaaccggga agcgggcttc cggatcgctg gtctctacga
       61 cgcggagggg accgccctcg agaagcagtt cgtgttcacc ggtgtggcgg ccctccagca
      121 gatggtggac gcggacgtgg tgaccgaagt gtcggtcaag ctcgaggacg aggcgctggt
      181 tgcccaccgg tcgctggagc gcgacgtcaa gtcgcggctg acgagctttt tctccacgct
      241 ggacgtgatg gactggcagc agcttgagcc gcaggctgcc gcaatgttcg tgtacgtgga
      301 cagcgccata ctgatctggt tcatggtcat catgggcgcc ctgatcttcg gcctggtcaa
      361 cacgctgatc accgcggtca tggagcgtat ccgcgagttt ggcatgctgc gcgcggtcgg
      421 catgaagccg tctgcggtgg tgatgcaggt ggtgctcgag tccaccttca tcatgctggc
      481 cggcgtggcc gtgggcatct tcgtcggttg gctgctgtgc accgtctggc ttggcgacgg
      541 catcgacctc agccagtggg ccgaaggcat cgagatggca gggatgcgga gccggctgac
      601 accgaagctc atgggtcagg acgtcgttct ggtgaccgtg ctgtcgctgc tcttcggcgt
      661 cattgcggcg ctctatccgg cctggcgggc cgtcaagata aaacctttgg aggccattcg
      721 aagatgaacg acctggttgt gcgttgtcag tccatctcgc gaacctatca ggacgacacg
      781 gtgcctgttc gggcgcttgt ggatgtggat ttcgaggtgg cccgaggtga gttcgtcagc
      841 cttgccggcc cgtccggctc gggcaagtcc acgctgctga acatcatcgg cggcctcgac
      901 cggcctgatt ccggtaccgt ggaggtggat ggtgtgctgc tcaacgagct ctccgagagc
      961 gacctctcgg acttgcggct gagaaagctg ggattcgtct ttcaggctta caatctgatc
     1021 cccgtgctct cggcgcggga gaacgttgag ttcatcatgc agctgcaggg ggttcccgcc
     1081 ggggagcggc gatcgcgcgc agaagccatg ctggaggaac tcggtctcga aggtatgacc
     1141 gaccgtcgcc ctggcgagat gtccggcggt cagcagcagc gggtggcggt ggcgcgcgcc
     1201 atcgtcaccc atccggtgct gctgctggcg gacgaaccca gcgcgaacct ggattcccac
     1261 accacggaag acctgctcaa gctgctccgg cggctgaacg aagaacacgg ggtgacgatc
     1321 gtcacggcca cccacgatcc gctggtgatg agctatgccc gacgtcaggt aaggctcagg
     1381 gacggtcaga tcatcgagga cagcgcccgc gaagcggcat gaagactctg cagctgccga
     1441 ccgctctggc gctgaccgtg ctcgcgggta cggccgcagg gcttgagctc gatgtccgtg
     1501 gcaagctctt cggg
//
LOCUS       DBNHOX010000467         8044 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1643976, whole genome shotgun sequence.
ACCESSION   DBNHOX010000467 DBNHOX010000000
VERSION     DBNHOX010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8044)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8044)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8044
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1643976"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="AC16UZ_10330"
     CDS             complement(<1..677)
                     /locus_tag="AC16UZ_10330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6247122.1"
                     /translation="MRDSEILQRLEKRLGTAHARERLGVETAHESRLFSHGIRFFHPE
                     NWYSVHSIIRNSLKVSGLYARASRNALDHRVVENHVVLDALPAGLDGLRVLHLSDLHV
                     DVSDELLHAIIERVRELTYDVCVLTGDYRFRTYGAIDDALRGMARLRSNLDGSVYAVF
                     GNHDSVTMLPALEDMGIRMLMNESHAHRHGDDHLYLAGIDDAHFFGVHDIEKALAGVP
                     DTVPRILL"
     gene            complement(722..1522)
                     /locus_tag="AC16UZ_10335"
     CDS             complement(722..1522)
                     /locus_tag="AC16UZ_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6247123.1"
                     /translation="MSDSEIVLCEIDDGTATLILNRPEVLNAMNDELMGGICRAMERV
                     VEDESVRVVILTGAGRGFCAGADLARAAEGPADPDLQDAPDSGDLFNGAVSALMSCPV
                     PTIARVNGPAAGGGLGLALACDVAIAAESAIFVATFGPNLGIVPDLGSTWSIPMRVGR
                     ARALGMALLGDRITANQAEAWGLIWRSVPDEELDAEVGRVAMVLKRSSPSACTRIREC
                     VDAAIHHTFGDQLGLEMAHQGVLISRNMQEGARAFMEKRPPEFGGDRG"
     gene            complement(1644..2405)
                     /locus_tag="AC16UZ_10340"
     CDS             complement(1644..2405)
                     /locus_tag="AC16UZ_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247124.1"
                     /translation="MNLGWVSVGRHSVIFLMAALLMLGSSGLRAEQGDETWWSITPYI
                     WAMDTKLDLSWRDSPVGGTEIDFKDALGNLDAAFQVAVEGGRGRWSAFADVTFVKISD
                     ATGEDALRLKTKNKQVFLDAGVAFWPGGAGSPLSIYGGARYTGFDNTYRLQFQGDDVA
                     RRDSSADYFDALLGVRYRFDLSDRWSILTLGDVSFGESEGSWQAQALAAYTVGKRRMN
                     RIMFGYRYRDTEFDDAELTTEYGFHGPLVGFNFRF"
     gene            2660..3913
                     /locus_tag="AC16UZ_10345"
     CDS             2660..3913
                     /locus_tag="AC16UZ_10345"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6247125.1"
                     /translation="MLELMPAPTVYQDEDFSPFADDFSPESLPYRGMLYVTDRRPAGA
                     GKPPDPADPHYVGKRGEGLRAGVASIQLGEGGFTWEEAIRVSMASDRGEGYRLSVTGV
                     EELGFLEPSIEPFIEAPDFPLADEAFAALVNDKLAQSEQKDVYIYVHGYRVRFEYPVL
                     VATELWHFLGYEGAFIAFSWPSTPSYLAYFADAEVAVVSARQLRILLEFLAQSTDVRR
                     IHILGYSQGTRTVMQALHMLALKYEEDDRESLLERLRIGNVALVGSDIDRETMGMYVA
                     DGLMRVTERMTVYASAKDKALRISRRVLRRDRIGQLIPSDEITDELRGFLITTPEFEI
                     INVTDAEDATTENGHRYFRSSPWVSSDLLTSFRYGMTPGQRGLEILPETPIWQFPADY
                     PTRLRSALNDVVDDQASVLPATSGQ"
     gene            4005..4081
                     /locus_tag="AC16UZ_10350"
     tRNA            4005..4081
                     /locus_tag="AC16UZ_10350"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4039..4041,aa:Arg,seq:ccg)
     gene            complement(4108..4605)
                     /locus_tag="AC16UZ_10355"
     CDS             complement(4108..4605)
                     /locus_tag="AC16UZ_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247126.1"
                     /translation="MSIVTRHLERRQRGRVGSPMKTILIALAAVVTLTGCDFIPFSGG
                     KLEGMLAEVPGDWTTIAQPDVIQLETQPSDPYSVKLWIVGKGPLLYVHAGANRAAWVE
                     HIEANANVRVLIDGNLYELVAERVTDQGEFDAFGDLYEAKYGNRPRNEDVDEAYLFRL
                     GARSG"
     gene            4751..5383
                     /locus_tag="AC16UZ_10360"
     CDS             4751..5383
                     /locus_tag="AC16UZ_10360"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6247127.1"
                     /translation="MQLYTVPLAPNPTKVMLYIAEREDLGTEMGIEQVVVNPVKGQQK
                     APEHLARNPFGTLPVLELDDGSYLVESLAIVQYLEDKFPHGALLTGTPEEKGKARDVE
                     RIVDLRVAGPMAGYVHATKSPLGLPPDPERAEQLSSSIQTPLDYLEGLLSDGRPLLLG
                     EGVSIADCTLQAALQFLRYVEADLLGERSKLREWDLRYRARPAAKRVLRW"
     gene            complement(5510..6331)
                     /locus_tag="AC16UZ_10365"
     CDS             complement(5510..6331)
                     /locus_tag="AC16UZ_10365"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6247128.1"
                     /translation="MGRLEGKRAVIFGASMDGNIGQVMARRFRDEGAQVLVASRNAAA
                     SAAFAESIGGYSAACDITRRDDVFALASHAKDALGGVDVAVNSTGLAMGGPFLEFGED
                     DLDTMIALQFKGSFFFLQAMVGAMLDKPEPGGSIIQISSAVAHRSTTVDLGFDAYMGT
                     KAGIDHVVRCVANQFGRQGIRANSIAAGHTDTPMHHDAYGGDIPDWMKAAFSDATPMG
                     RYGTCDDVAAGAVWLASDECFMTGDALQINGGLTLRRNPLKEDLDRYEREWAGRG"
     gene            6429..7805
                     /locus_tag="AC16UZ_10370"
     CDS             6429..7805
                     /locus_tag="AC16UZ_10370"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6247129.1"
                     /translation="MEEPKPKPLARSSTPSAQELVHRDGRPVPPVFELESPRFLGDED
                     IPFERYTSQEFFNREMERMWTRTWQWACREEHVADPGDYYVYEIGPHSVLIVRDALGE
                     VRAYVNACLHRGTKFRSGGSDGAFSEIRCPFHGWTWELDGSLKRVPYAWDAPHVSERE
                     FSLPRVAVGLWGGFVFVNLDPDPPPFDECLAPIPEHLENFDLSKRFVELHIEKELNCN
                     WKAAIEAFIENYHTQETHPQLLYGSDDEGTQYDVFTDHVSRFFCAFGVPSPSIDPPPT
                     EQELVDMMLVGDRSVLGDAVTVKPGETARIVMARVLRQTLGEKYAADLGRYTDTEMID
                     VSQYGLFPNMVLFPQLSLPMVYRFRPIGENPDRTLFELLILRPAPDQGRPPAPARPHR
                     LEERDSFTTVPGFDTSLGEVYDQDTGNLRAQQEGFRASRKRGATLLNYQEVRVRHLHQ
                     TLDDYLQQ"
     gene            complement(7813..>8044)
                     /locus_tag="AC16UZ_10375"
     CDS             complement(7813..>8044)
                     /locus_tag="AC16UZ_10375"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6247130.1"
                     /translation="LPAGCYGSEPMFAAAEGATREDDGYLLEVVYDGYSHRSELQVYR
                     ADDVNERVCTLHLSHHLPHQFHGYFSPRTFLA"
BASE COUNT         1491 a         2663 c         2482 g         1408 t
ORIGIN      
        1 agcaggatcc tgggcaccgt gtcgggaacg ccggccagag ccttctcgat gtcgtgcacg
       61 ccgaagaagt gagcatcgtc gatcccggca aggtagaggt gatcgtcacc gtgccggtgg
      121 gcgtgacttt cgttcatgag catgcgaatg cccatgtcct ccagggcggg cagcatggtc
      181 acgctgtcgt ggttaccgaa gacggcgtag accgatccgt ccaggttgct gcgcagccgg
      241 gccatgccgc gcagggcatc gtcgatggcg ccgtaggtgc ggaaacggta gtcgccggtc
      301 agcacgcaga cgtcgtaggt cagctcccgg accctttcga tgatggcgtg cagcagctcg
      361 tcgctcacgt ccacgtgcag atccgagagg tgcaggaccc gcagcccgtc tagaccggcg
      421 ggaagagcgt cgagcaccac gtgattctcc accacccgat gatcgagagc gttcctgctg
      481 gcgcgggcat acaggcccga gaccttgagc gagttgcgaa tgatggaatg aaccgagtac
      541 cagttctccg ggtgaaaaaa gcggatccca tggctgaata gccgagactc gtgagccgtc
      601 tccacgccga ggcgctcacg ggcgtgggcg gtgcctaggc gtttttccag gcgttgcaga
      661 atctcgctgt cgcgcatctg aagattatat atgccacgcc cctgcccgct gacgtttccc
      721 gctatccccg atcgccgccg aactcggggg ggcgtttttc catgaacgcc ctggccccct
      781 cctgcatgtt acgcgagatg agcactccct ggtgcgccat ctccagccca agctgatccc
      841 caaaggtgtg gtgaatggct gcatcaacgc attcgcgaat gcgggtgcac gcgctcggcg
      901 aggagcgctt cagaaccatc gccacgcggc cgacttccgc gtcgagctcc tcatccggca
      961 ccgagcgcca gatgaggccc caggcttcgg cctggttcgc ggtgatgcga tcgcccagca
     1021 gcgccatccc cagggcacgg gcacgcccaa ccctcatcgg tatgctccag gtggagccga
     1081 gatcgggcac gatgccgaga ttggggccga aggtcgccac gaagattgcc gactccgcgg
     1141 cgatggcgac gtcgcaggcc agcgccagcc cgagcccgcc gccggccgca ggaccattga
     1201 cccgagcgat ggtcggcacc gggcagctca tcagcgcgct gaccgcgccg ttgaagagat
     1261 cgccgctgtc cggggcgtcc tgcaggtccg ggtctgccgg cccctccgcc gcgcgggcga
     1321 gatccgcgcc ggcgcaaaag ccgcgccccg cgcccgtcaa gatgaccacg cgcacgcttt
     1381 cgtcttcgac aacccgttcc atggcccggc agatgccgcc catgagctcg tcgttcatgg
     1441 cgttgaggac ctcgggccgg ttcagaatga gcgtggctgt gccgtcgtca atctcgcaga
     1501 ggacgatttc tgagtctgac ataggactcc cccatgaaga gcggatgaat cagctgcatc
     1561 ccatggaacc agaaacgtcc cggagcggac aagggctacg cgccatcgcg ccgggcgggg
     1621 aggtcaataa cccgttggcg gtctcagaac cggaagttga aaccgacgag gggcccgtgg
     1681 aacccgtact ccgtggtcag ctcggcatcg tcgaactcgg tgtcccgata gcgatacccg
     1741 aacatgatcc ggttcatgcg ccgcttgccc accgtgtagg cggccagtgc ctgagcctgc
     1801 cagctgcctt cggattcgcc gaagcttacg tccccgaggg tgaggatgga ccaccgatcc
     1861 gacagatcga aacggtagcg aacgcccaga agagcatcga agtagtcggc cgacgaatct
     1921 cgacgggcga catcgtcgcc ctggaattgc agacggtagg tgttgtcgaa cccggtgtag
     1981 cgcgctccgc catagatgct cagtggcgag ccggcgccgc ccggccagaa ggcaaccccg
     2041 gcgtccagga acacctgctt gttcttggtt ttcagccgca acgcatcctc accagtcgcg
     2101 tccgagatct tgacgaaggt cacatccgca aaggcgctcc agcgaccccg ccccccttcc
     2161 acggccacct ggaacgccgc atcgagattg cccagcgcgt ccttgaaatc gatctcggtg
     2221 ccgccgacgg gcgagtcccg ccagctgaga tccagcttcg tatccatggc ccagatgtag
     2281 ggcgtgatgg accaccaggt ctcgtcgcct tgttccgcgc gaagcccgct gctgccaagc
     2341 atcagcaatg cggccatcag gaagatgacc gaatgccgtc ccacgctgac ccatccaaga
     2401 ttcataactg caagtcctct cgctacgctc ggctagtccc aacaacgtgc cacgtacctg
     2461 aaatcagcag gccctcgacg acgcgcatca tactggacct gtgctgatgt ggcatcattg
     2521 acctcttcga gaatgaaaac gtcagccatc aagaggtcgc caagccttga ttactgagcg
     2581 agccaaccca tcgctacgct tgtttgctgc cgtggccatg ttggctgctc tgaccgcatg
     2641 cgctgcaaaa gggccctacg tgctggagct gatgcccgcg cccaccgttt accaggacga
     2701 ggacttcagc cccttcgccg acgacttcag cccggaatcg ttgccgtatc gcggcatgct
     2761 gtacgtaacg gaccgcaggc cggctggtgc cggaaagccg cccgatcccg cagacccgca
     2821 ctacgtcggc aagcgcggcg agggccttcg ggcaggcgtc gcctccatcc agctcggcga
     2881 gggcggcttc acctgggaag aagccattcg ggtgtcgatg gcgagcgatc gcggcgaggg
     2941 ataccggttg agcgtgaccg gcgtggaaga gctcgggttc ctcgagccga gcatcgagcc
     3001 attcatcgaa gcgcccgatt tcccgctggc ggacgaagcg ttcgcggcgc tggtcaacga
     3061 caagctcgcc cagtccgagc agaaggacgt ctacatctac gtgcacggct accgggtgcg
     3121 gttcgaatac ccggtgctcg tggccacgga gctctggcac ttcctgggct acgaaggggc
     3181 attcatcgct ttctcgtggc cttccacgcc gagctatctc gcctacttcg ccgacgccga
     3241 ggtggcggtg gtctccgccc gacagctgcg catcctgctg gaattccttg cgcagagcac
     3301 cgacgtgagg cggatccaca ttctgggcta cagccagggc acccgcacgg tcatgcaggc
     3361 cctgcacatg ctggcgctga agtacgaaga ggacgatcgc gagtcgctgc tcgagcggct
     3421 gcgtatcggc aacgtggccc tcgtcggcag cgacatcgat cgggagacaa tggggatgta
     3481 cgtagccgac ggcctgatgc gggtgacgga gcgtatgacc gtctacgcgt cagccaaaga
     3541 caaggctctg cgcatctcgc ggcgcgtatt gcggcgcgat cggatcgggc agctcattcc
     3601 ctcggacgag atcaccgacg agcttcgggg cttcctcatt accacaccgg aattcgagat
     3661 catcaacgtc accgatgccg aagacgccac caccgagaac ggccaccgtt actttcgaag
     3721 cagcccgtgg gtgagcagcg atctgctcac cagcttccgc tacggaatga cgccagggca
     3781 acgcggtctc gagatcctgc cggagactcc gatctggcag tttccggccg attacccgac
     3841 gcgcctgcgc tctgccctca acgacgttgt cgacgaccag gcatcggtgt tgccggcaac
     3901 gtccggccag tagctgccca gggcgatcac gtcaccagag acgtccagcg cctgccgccg
     3961 ggccctgtcg atgtggtcgg ttgtgcccta taatcgccgc ccccgcgccc gtagctcagc
     4021 tggatagagt actggcctcc gaagccaggg gtcgcgcgtt cgaatcgcgc cgggcgcgcc
     4081 aatcaccgcg ggtgctgggc aggcagatca gcctgatcga gcaccgagac gaaacaggta
     4141 agcctcgtcg acgtcctcgt ttctcggccg gtttccgtac ttggcctcgt acagatcccc
     4201 aaaggcgtcg aactcgccct ggtcggtcac gcgctcggcc accagctcgt agagatttcc
     4261 gtcgatgaga acccttacgt tggcgttcgc ctcgatgtgc tccacccagg cggccctatt
     4321 cgccccggcg tgcacgtaga gcagcgggcc cttgccgacg atccatagct tgaccgaata
     4381 ggggtcgctg ggctgagtct cgagctggat cacgtctggc tgcgcaatcg ttgtccaatc
     4441 cccaggtact tccgccaaca tcccctccag cttccctccc gagaaaggaa tgaagtcgca
     4501 gccggtcaac gtcaccacgg ccgccaacgc gatcaggatc gtcttcattg gggagccgac
     4561 gcgtccccgc tgccgtcttt cgagatgccg ggtgacaata ctcatctcac cgtcctcctg
     4621 tattgattgc cggagatcgc cgttcgcgat catttcaaac cattcgtgcc tggacaccga
     4681 acatcctatg gtggcatgtt cccgggcccg gcgcaggctg cggggccgag ccgtcattgg
     4741 aggatctttc atgcagcttt acacggttcc cctggccccc aacccgacca aggtgatgct
     4801 ctacatcgcc gagcgggagg atctcggcac cgaaatgggc atagagcagg tcgtcgtcaa
     4861 cccggtcaaa ggtcagcaaa aagcgccaga gcatctcgcc cgaaatccct tcggcacgct
     4921 gcccgtcctg gagctcgacg acggctcgta cctcgtcgaa tccctggcga tcgtccagta
     4981 tctggaggac aagtttcccc acggggcctt gctgacggga acccccgagg agaagggcaa
     5041 agcgcgcgac gtcgagcgca tcgtcgacct gcgtgtggcc ggccccatgg caggatacgt
     5101 gcacgccacg aaatccccgt tgggcctgcc gccggacccg gaacgagccg agcagctctc
     5161 atccagcatt cagacccccc tcgactatct ggagggattg ttgtcggacg gtcgtccgct
     5221 gctgctggga gagggcgtca gcatcgccga ctgcacgctg caggccgccc tccagtttct
     5281 tcgctatgtc gaagcagacc tgctgggtga gcgttcgaag ctccgcgaat gggacttacg
     5341 ctaccgagcc cggcccgcgg ccaagcgcgt gcttcgctgg tagcaaacgc catgatgttc
     5401 cagacacccc ggctgcattt ccgattgttc gaggtccagg acctcgagga gctggcgagc
     5461 atcaacgccg accaggagac atcccgccac gtcggcgacg gcagagcgct cagccgcgcc
     5521 cggcccactc acgctcgtag cggtcaagat cttccttgag ggggttgcgg cgcagggtga
     5581 ggccgccgtt gatctgcaga gcatctcccg tcatgaagca ctcgtcgctg gcgagccaga
     5641 cggcgccggc ggcaacgtcg tcacaggtgc cgtatcggcc catgggggtc gcatcggaaa
     5701 acgcggcctt catccagtcc gggatgtcgc caccgtaggc gtcatgatgc atgggcgtgt
     5761 ccgtatgacc ggccgctatg ctgttcgccc ttatgccctg gcgaccgaac tgattggcca
     5821 cgcaacggac cacgtgatcg atcccggcct tagtgcccat gtaggcatca aaaccgagat
     5881 cgacggtcgt ggagcgatga gcaactgcag agctgatctg gatgatcgat cccccaggct
     5941 ccggcttgtc gagcatggcg cccaccatgg cctgcaggaa gaagaacgag cctttgaact
     6001 gcagcgcgat catggtatcc agatcgtctt caccgaactc gaggaacggc ccgcccatag
     6061 ccaggccggt ggagttgacg gccacgtcga cgccacccag cgcgtctttg gcgtggctgg
     6121 ccagagcgaa gacgtcgtcg cgtctggtga tgtcgcatgc cgccgagtag ccgccgatgg
     6181 attccgcgaa tgcggccgac gctgcggcgt tgcggctggc caccagcacc tgggcaccct
     6241 cgtcacgaaa ccgcctggcc atcacctggc cgatgtttcc atccatcgat gcgccgaaga
     6301 tcacggcgcg cttgccttcg agtcgtccca tcgtcccctc cctgtccaac actggcgagc
     6361 tgacgttcat tgtacagggg ttcaaatgac cttaggctag ccgagccact ggtccgggtc
     6421 gacagacgat ggaagaaccc aagccgaagc cgctggctcg ttcgtccaca ccatccgcgc
     6481 aggagctggt gcaccgagac ggacgcccgg tgccgcctgt tttcgagctg gaaagccccc
     6541 gattcctggg cgacgaagac attcccttcg aacgttacac gagccaggaa ttcttcaatc
     6601 gggagatgga gcggatgtgg acccgaacgt ggcaatgggc ctgccgcgaa gagcacgtcg
     6661 ccgaccccgg cgactactac gtgtacgaga tcggtccgca ctcggttctg atcgttcggg
     6721 atgcgcttgg ggaggtcaga gcctacgtca acgcgtgcct tcaccgcgga accaagttca
     6781 ggtcaggcgg ctccgatggc gctttcagcg agatacgctg cccttttcac ggctggacct
     6841 gggagctgga cggcagcctg aagcgggtgc cctacgcctg ggacgcgccc cacgtcagcg
     6901 agcgcgagtt ctcgctgccc cgggtggccg ttgggctgtg gggcgggttc gtcttcgtca
     6961 acctcgatcc cgacccgccg ccttttgacg agtgtctcgc gccgattccc gagcatctcg
     7021 agaacttcga tctgtcgaag cgtttcgtgg agctgcacat cgagaaggag ctcaactgca
     7081 actggaaggc cgccatagag gccttcatcg agaactacca cacccaggag acccaccccc
     7141 agctgctcta cggcagcgac gacgagggca ctcagtacga cgtcttcacc gaccacgtgt
     7201 cccgcttctt ctgcgccttc ggagtcccaa gccccagtat cgacccgccc ccgacggagc
     7261 aggagctggt ggacatgatg ctggtcggtg accggtcggt gctcggcgac gccgtgacgg
     7321 taaagcccgg agaaaccgct cgaatcgtca tggcccgggt gctccgtcag accctgggcg
     7381 agaaatatgc agccgacctc ggccgttaca ccgacaccga gatgatcgac gtctctcagt
     7441 acggcctgtt tccgaacatg gtgctgtttc cgcagctgtc gctgccaatg gtctaccgat
     7501 tccgccccat cggcgagaac ccggaccgga cgctgttcga gctgctgatc ctccggccgg
     7561 cacccgacca gggtcgccca ccggctccgg cgcgccccca tcgccttgag gagcgcgaca
     7621 gcttcaccac ggtgcccggc ttcgacacca gcttaggcga ggtgtacgac caggacaccg
     7681 gcaacctccg cgctcagcag gaagggttcc gggcgtcacg caagcgcggc gcgaccctgc
     7741 tgaactatca ggaagtccgg gttcgccacc tgcaccagac cctcgacgac tatctgcagc
     7801 agtagaagcc gtctaggcga gaaacgtccg gggcgagaag tagccgtgaa actgatgggg
     7861 caggtgatgg ctaaggtgca gggtacagac ccgctcattg acgtcgtcgg cccggtagac
     7921 ctgcagctcg ctgcggtgtg agtagccgtc gtaaacgact tccagaaggt agccgtcgtc
     7981 ttcgcgggtc gcgccctctg cagccgcgaa catgggctcc gagccgtagc agccggccgg
     8041 cagg
//
LOCUS       DBNHOX010000468         2392 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1644051, whole genome shotgun sequence.
ACCESSION   DBNHOX010000468 DBNHOX010000000
VERSION     DBNHOX010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1644051"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="AC16UZ_10380"
     CDS             complement(<1..608)
                     /locus_tag="AC16UZ_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015275390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR6247131.1"
                     /translation="MEIFSTVIAVIIVGLLISAFRVLREYERGIVFMLGRFYKVKGPG
                     LIIIVPVIQQMVRVDLRTIVLDIPSQDLITRDNVSVKVNAVLYFRVVDPERSIINVED
                     YMEATGQLAQTTLRSVLGQHELDELLAERERLNSDVQAILDKQTDQWGIKVANVEIKH
                     VDIDESMIRAIAKQAEAERERRAKVIHAEGEAQAASKLVEAA"
     gene            complement(612..1976)
                     /locus_tag="AC16UZ_10385"
     CDS             complement(612..1976)
                     /locus_tag="AC16UZ_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR6247132.1"
                     /translation="MKAWLPIIIACAGLALGFGEARPATGAQPAADGVVWLVDLDGPL
                     GPATADLIIRSLDDAQEAGARALVIRMDTPGGLDKAMRGLIKEMLSADLPVVTFVGPD
                     GARAASAGTYIAYASHIAAMAPATNIGSSTPVSIAPTPQMPDPRDAGETEGDAGRAPS
                     GDAMTRKVVNDAVAYLQGLAELRGRNIDWAEETVRLGANLRASEALEANVVDLITRDV
                     SSLLADIDGWVVELPNRTVTLDVANARVHTVETDWQHELLEVITDPTIAYGLLIFGVY
                     GLILEFYNPGMVFPSVIGIICLLLGAYGLQMLPVNYAGLALIVVGIGMMIAEVFTPTM
                     GVLGLAGVVAFVAGSMILMDTEAPGYGIPITVVAAFAAASAGLTIFAIGAAMKARRAK
                     IVTGVEAMIGASGEALEDFQGTGHVRAFGEIWEARCSEPVAAHAKVRVTEVDGLILVV
                     QPED"
     gene            complement(2086..>2392)
                     /locus_tag="AC16UZ_10390"
     CDS             complement(2086..>2392)
                     /locus_tag="AC16UZ_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6247133.1"
                     /translation="ASFAATWPLREMEETITKDISSHVRERVARFGITPDRVAVLYGR
                     PAVEIRAYAAKIGADLIVIGSHGRHGVPGVLLGSTANAVLHGAECDVLTVRVRPAEG"
BASE COUNT          434 a          798 c          728 g          432 t
ORIGIN      
        1 tgagcagcct ccaccagctt cgaggcggcc tgcgcttcgc cctccgcgtg aatgaccttg
       61 gctcggcgtt ctctctcggc ttccgcctgc ttggcaattg cccggatcat gctctcgtcg
      121 atgtcgacgt gcttgatctc cacgttggcg accttgatgc cccactgatc ggtctgcttg
      181 tccaggatcg cctggacgtc cgagttgagc cgctcccgct ccgccagcag ctcgtcgagc
      241 tcatgctggc cgagcactga ccggagcgtg gtctgggcga gttgcccggt cgcttccatg
      301 tagtcttcga cgttgatgat cgaccgctcc ggatcgacga cccggaaata gagaacggcg
      361 ttgaccttga cggacacgtt gtcgcgggtg atgagatcct gggaggggat gtcgagcaca
      421 atggttctca agtccacccg aaccatctgc tgaatgacag gaacgatgat gatcagaccg
      481 ggccccttga ccttgtagaa gcgtccgagc atgaagacga tgccccgctc gtactcccgc
      541 aggacccgga aagcgctgat cagaagacca acgatgatga ccgcgatgac tgtcgaaaat
      601 atttccatta atcaatcctc cggttgaacg accagaatca gcccgtctac ttcggttacg
      661 cgcactttcg cgtgtgccgc cacgggctcg ctgcagcgtg cctcccagat ctcgccgaat
      721 gctcgaacgt ggcccgtccc ctgaaagtcc tccagcgctt ctcccgaggc gccgatcatt
      781 gcttccacgc cggttacgat cttcgcccga cgggccttca tcgcagcccc gatggcaaaa
      841 atcgtcaggc ccgcactggc tgccgcgaat gcggccacca cggtaatcgg gatgccgtag
      901 ccgggcgcct cggtgtccat caggatcatc gagcccgcga cgaacgcgac gaccccggcg
      961 aggcccagca cacccatggt tggcgtgaag acctccgcga tcatcatgcc gatgccgacc
     1021 acgatcagcg ccaggcccgc gtagttgacc ggaagcatct gcagaccgta tgcgcccaga
     1081 agcaggcaga tgattccgat caccgaggga aagaccatcc cgggattgta gaattccagt
     1141 atcaggccgt agacgccgaa gatcagcagg ccgtaggcaa tcgtcggatc cgtgatcact
     1201 tcgagaagct cgtgctgcca gtctgtctcc acggtgtgca cgcgcgcgtt tgccacatcg
     1261 agcgtgacgg tccgattggg aagctccacc acccacccgt cgatgtcggc aagaagcgag
     1321 gagacgtccc gcgtgatgag atccaccacg ttggcttcca gagcttccga ggcgcgcagg
     1381 ttggcgccaa gccgcaccgt ctcctcggcc caatcgatat tccggcccct gagctccgcc
     1441 agcccctgca gataggctac ggcgtcgttc acgaccttgc gtgtcatggc gtcccccgac
     1501 ggtgcccgcc cggcgtcgcc ttccgtttcg ccggcgtcac gcgggtcggg catctgaggc
     1561 gtcggcgcga tgctgacggg cgtagaggaa ccgatgttgg tagccggtgc catggctgcg
     1621 atgtggctgg cgtaagcaat gtaggttccg gcgcttgccg cacgggcgcc gtccggcccg
     1681 acgaaggtga ccacgggcag gtctgccgaa agcatctcct tgatcagccc gcgcatggcc
     1741 ttgtccagcc ctcccggtgt gtccattcga atcaccagcg cgcgggcccc ggcctcctgg
     1801 gcatcatcga gcgagcggat gatgagatcc gccgtggcag gaccgagggg accgtcgaga
     1861 tccaccagcc acaccacacc gtccgccgcg ggctgcgccc cggtggctgg acgggcttcc
     1921 ccgaagccca gggcaagtcc ggcacaagcg atgataatcg gtagccaggc cttcatggtg
     1981 ggagcagcaa tgtccaagtt cccgaacgtt gcagcattgt acgccttatt gccgggatcg
     2041 gctgatcgaa ctcgcccacg gcctagccgc gggcaatgag cggacctaac cttctgccgg
     2101 ccgcacccgc acggtcagca catcgcattc ggcgccgtgc agcactgcgt tggccgtgga
     2161 accaagcagc acgccgggca ccccgtggcg accgtgagac ccgatgacga tcaggtcggc
     2221 tccgatcttc gcggcgtaag cgcgaatttc gaccgccggc ctgccgtaga gtacggcaac
     2281 gcggtccggc gtgatgccga accgcgccac acgctcacgc acgtggctgg agatatcctt
     2341 tgtgatggtt tcctccatct cgcgcagcgg ccaggtggca gcgaacgatg cg
//
LOCUS       DBNHOX010000469         4341 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1772169, whole genome shotgun sequence.
ACCESSION   DBNHOX010000469 DBNHOX010000000
VERSION     DBNHOX010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1772169"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /gene="amrS"
                     /locus_tag="AC16UZ_10395"
     CDS             <1..1018
                     /gene="amrS"
                     /locus_tag="AC16UZ_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HIR6247134.1"
                     /translation="CDVCPRHCRLREGQRGLCYVRQCLDGEVKLVSYGRSSGFCVDPI
                     EKKPLNHFYPGSSVLSFGTAGCNLACKFCQNWDMSKSREMDTLADRAMPERLAEAAVD
                     LACRSVAFTYNDPVIFLEYAVDVADACREVGVKSVAVTAGYVDPEPRREFFSAVDAAN
                     VDLKAFSERFYHKICGGHLAPVLETLEFIRHETNTWLELTTLLIPGENDSDEELDEMT
                     GWVVEHLGPDVPMHFTAFHPDWKMRDVPSTPPGTLTRARSIARRNGVHFAYTGNVHDP
                     AGSSTYCSACDALLIERDWYQLGRWGLDGAGRCSACGEPLAGHLEGPPGQFGSRRIPV
                     RLDQ"
     gene            1109..1381
                     /locus_tag="AC16UZ_10400"
     CDS             1109..1381
                     /locus_tag="AC16UZ_10400"
                     /inference="COORDINATES: protein motif:HMM:NF040598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpp/OprI family alanine-zipper lipoprotein"
                     /protein_id="HIR6247135.1"
                     /translation="MKNLFFAMIATLLLAGCATTEQVEALQNRVSALENQIGSANDAA
                     GDAASAASRAASDAAAAQSTAERALDAANAANERAERISETCCSRK"
     gene            complement(1389..2285)
                     /locus_tag="AC16UZ_10405"
     CDS             complement(1389..2285)
                     /locus_tag="AC16UZ_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR6247136.1"
                     /translation="MSVPTSRTSAAALLACLLVAAAATEAASYRLPEGGEDVIGASIS
                     LTLAYEDTFAGVAQQFGVGYQELVDANPGVDPWLPGDGARIVMPTAYVLPSAPREGIV
                     INVAEYRLYYYPPDRGSVITFPVGVGRMDFPTPLISTKVVTRIENPSWTPTDAARREH
                     AEMGDPLPHVVPPGPDNPLGHLAIQLSAPGYFIHGTNKPFGVGQGVSHGCVRLYPGHI
                     ETLSELVPNGTPVYIVNEPYKVGWRDGELWIEAHRNLYGENQASELVALIVDATEKRD
                     ASVDWRRVDEVSAALTGIPVRL"
     gene            2433..3272
                     /locus_tag="AC16UZ_10410"
     CDS             2433..3272
                     /locus_tag="AC16UZ_10410"
                     /inference="COORDINATES: protein motif:HMM:NF046782.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR6247137.1"
                     /translation="MPINPDAVGAEGRPVRRSWSSKDALLYAVGVGSGTDELAFTTEN
                     TKDVAQRVLPTFAVIIGGGGVPMNAIGSFNPALMVHGEQGIELYDEIPPDGEIDSVGK
                     VTAIWDKGSAAVLEFESTSTNVATGKPLLKTRTSLFCRGEGGWGGDRGPSEKVEFPDR
                     EPDHQVTYATREDQALTYRLSGDRNPLHSDPSFAALGGFEKPILHGLCTWGFTGRALL
                     HTLCDGDPSRFKSMNSRFSKPVIPGDELTISIWVVDEGRALFRTTNQNGDAVIDQGVF
                     TYA"
     gene            3298..3690
                     /locus_tag="AC16UZ_10415"
     CDS             3298..3690
                     /locus_tag="AC16UZ_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247138.1"
                     /translation="MIELVLRLSLWISLPLNAGAAYILAFPSSWPGQLLGLPAAPDPV
                     YAYLAAYLVALLGLAYGWMALQPSIVRPLLWLGALGKGGAFAVAAALWLAGLARAPLA
                     AAVTVDLLLAAFWIAWLTGTRGKASADG"
     gene            complement(3744..>4341)
                     /locus_tag="AC16UZ_10420"
     CDS             complement(3744..>4341)
                     /locus_tag="AC16UZ_10420"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR6247139.1"
                     /translation="VSKGVDEPVVETIREALEIFRELGATVVDIEVPASAATLVRGWG
                     VTCGMECARAHRDFYPDRKAEYGPVLAAFIDYGRAVSREDYGLLETVRAEFRDGLNGC
                     LESADVIIAPCMPELPPPADALADGPRRSSDRADLITFTAPFNYSGHPTITLPAKLER
                     GSLPRAFQLIARPLGEPTLVRAGSAFESALGLDAHPIP"
BASE COUNT          760 a         1414 c         1402 g          765 t
ORIGIN      
        1 gtgcgacgtt tgtccgcgcc actgccgcct gcgcgagggc cagcggggac tgtgctacgt
       61 gcgccagtgt ctcgacggtg aggtgaagct cgtcagctac ggcagatcgt cggggttctg
      121 cgtggacccc atcgagaaga aaccgctgaa ccacttctac cccggcagca gcgtgctctc
      181 cttcggcacc gcgggctgca acctcgcctg caagttctgc cagaactggg acatgagcaa
      241 gtcccgcgag atggacaccc tggccgatcg agccatgccg gagcggctgg ccgaagccgc
      301 ggtggatctg gcgtgccgaa gcgtggcttt cacctacaac gacccggtca tcttcctgga
      361 gtacgccgtc gacgtcgccg acgcctgtcg cgaagtcggc gtgaagtccg tggccgtgac
      421 ggccggatac gtggaccccg agccccggcg cgagtttttc tccgcggtgg acgccgccaa
      481 cgtcgacctc aaggcgttca gcgagcgctt ctaccacaag atctgcggcg gccacctcgc
      541 gccggttctg gagacgctgg aattcatccg ccacgagacc aacacctggc tcgagctgac
      601 cacgctgttg ataccgggcg agaacgactc cgacgaggag ctcgacgaga tgacgggctg
      661 ggtcgtcgag cacttgggtc ccgacgtgcc catgcacttc accgcctttc acccggactg
      721 gaagatgcgc gacgtgccca gcacgccccc aggcaccctg acccgggctc gcagcattgc
      781 caggcgcaac ggggtgcact tcgcctacac cggcaacgtg cacgatcctg ccggctccag
      841 cacctactgc tcggcctgcg atgccctgct catcgagcgc gactggtatc agctgggccg
      901 ctggggactg gacggggccg gacgctgcag cgcctgcggc gagccgctgg ccggacatct
      961 cgaagggcct ccgggccagt tcggaagccg gcgcatcccg gtgcgactcg atcaatgagc
     1021 ataatttcag cgcaatcaac tgatgacaaa gccgctgact atggtagggt tcgcttccga
     1081 gaaaaacctg gctgagtgag gcccccaagt gaaaaatctt ttctttgcca tgatcgccac
     1141 cctgctgctc gcaggctgcg caactaccga acaggtcgag gctctccaga accgcgtcag
     1201 cgcgctggag aaccagattg gctccgctaa cgacgctgcc ggagatgccg cttctgctgc
     1261 ttcccgagcg gcgagcgacg ccgcggctgc tcagtccacc gcggagcgtg cgctggacgc
     1321 cgccaatgcg gccaacgagc gggcggagcg gatttcggag acctgctgct cccggaagta
     1381 gtgctccgtt aaagcctgac cggaatcccg gtcagtgctg cactgacctc gtcgacgcga
     1441 cgccagtcca cactggcgtc gcgtttttct gtggcatcga caatcagcgc aacgagttcg
     1501 gaagcctggt tttcgccata taggttgcgg tgcgcctcta tccagagctc gccatcgcgc
     1561 catcccacct tgtagggctc attgacgatg tatacgggcg tcccgttcgg aacgagctcc
     1621 gacagcgtct ctatgtggcc cggatacagg cggacgcagc cgtggctgac accctgacct
     1681 acgccgaacg gtttgttggt gccgtgaatg aaatatccag gcgcgctcag ctgaatggct
     1741 agatgcccca gagggttgtc aggccctggc ggcaccacgt ggggaagcgg atcacccatc
     1801 tctgcatgct cccgccgggc cgcatctgtt ggggtccagc tagggttctc tatcctggtg
     1861 accaccttgg tgctgatgag cggcgtgggg aagtccatcc gccctacccc caccgggaac
     1921 gtgatgaccg agccgcgatc aggcggatag tagtagagcc gatactcggc gacgtttatg
     1981 acgatccctt cccttggcgc ggagggcagc acgtaggcgg tgggcatgac gatacgggca
     2041 ccatctccgg gtagccaggg atccaccccg gggttggcat ccaccagttc ctgatagccc
     2101 acgccgaatt gctgagccac acctgcaaag gtgtcttcgt aggcgagcgt caggctgatg
     2161 ctggcgccga tgacgtcctc tccgccttcg ggcagccgat aggacgccgc ttcggtcgcg
     2221 gccgccgcaa caagcaggca ggccagcagg gctgcggcag aagtccgtga tgtcgggaca
     2281 gacaagtcgt tctcaaacca tcggccggga ggacgccatt gtccccatct ggattcccag
     2341 gtccagtccg ctgccgcggc tgcattcgcg ttgatcgcaa tggggcccac ggctatggta
     2401 gcgggcatcc atgtaagcgg gaggatcttc gcatgccaat caacccagat gccgtcggcg
     2461 ccgagggacg tccggtgcgc cgcagctggt cgtccaaaga tgctttgctc tacgctgtgg
     2521 gcgtcggttc aggcaccgat gagctcgctt tcaccaccga gaacacaaag gacgtggcgc
     2581 aaagggttct gcccaccttc gccgtgatca tcggcggcgg cggtgtgccg atgaacgcca
     2641 tcgggtcctt caaccccgcg ctcatggtcc atggcgagca gggcatcgag ctctacgacg
     2701 agatcccgcc ggacggagag atcgatagcg tgggtaaagt gaccgccatc tgggacaaag
     2761 gctccgcggc ggtgctggag ttcgaatcca catccacgaa cgtcgccacg ggcaagccgc
     2821 ttctgaagac acgaacgagc ctcttctgcc gcggcgaagg gggctggggc ggcgatcgag
     2881 ggcccagcga gaaagtggaa tttcccgacc gcgagccgga ccatcaggtc acctacgcga
     2941 ccagggagga tcaggcgctc acctaccggc tctcaggcga tcgcaacccg ctgcattcgg
     3001 atccttcctt tgcggcgctc ggcggcttcg agaagcccat actgcacggg ctgtgcacgt
     3061 ggggattcac aggtcgcgcc ctcctgcaca ccctttgcga cggagacccg agccgattca
     3121 agagcatgaa cagccggttt tccaagcccg tgatccccgg ggacgagttg acgatctcga
     3181 tatgggtggt ggacgaaggc agggctttgt tcaggacgac caatcagaac ggcgacgcgg
     3241 tcatcgatca gggcgtgttc acatacgcct gatagcgtgc gcctgcggcg agcggcaatg
     3301 atcgagcttg ttctgcggct gtcgctctgg ataagcctgc cgctcaacgc cggtgctgcc
     3361 tacatcctgg ctttcccctc gtcctggccg ggtcagctgc tgggcctgcc ggcagcgccc
     3421 gatcccgtgt acgcctacct tgcagcatat ctggtggccc tgctcgggct cgcctacggc
     3481 tggatggccc tgcaaccgag catcgtcagg cccctgctgt ggttgggcgc ccttggcaaa
     3541 gggggcgcct tcgcggtggc ggcagccctt tggcttgccg gtctggctcg cgctcccctc
     3601 gcggccgccg tgaccgtcga cctgctgctc gcggcgttct ggatcgcctg gctcacaggc
     3661 acgcggggaa aggcgagcgc tgacggttga tcgcttgatt tccgggtcag gcgtagccag
     3721 cgacgccggg cgtcggggcc cggtcaggga atgggatgcg cgtccaggcc gagagcgctt
     3781 tcgaacgcgc tgccggcacg aacgagggta ggctctccca ggggtcgcgc gatgagctga
     3841 aaggctctcg gcaggctgcc ccgttcgagc ttcgccggca gggtgatggt cgggtgaccg
     3901 gaataattga aaggcgcggt aaaggtgatg agatccgccc gatctgacga ccgtcgtggt
     3961 ccgtccgcca atgcgtcggc tgggggcggc aactcgggca tgcagggagc gatgatgacg
     4021 tcggcgcttt ccaggcaccc gttgagccca tcgcgaaact ccgcgcggac ggtttcgagc
     4081 aatccgtaat cctcccggga aaccgcccgc ccgtaatcga tgaaggccgc cagcacgggt
     4141 ccgtattccg ccttgcggtc gggatagaaa tcccggtggg cacgcgcgca ctccataccg
     4201 caggtgactc cccaccctcg cacgagcgtc gcggcactcg ccggcacctc gatatcgacc
     4261 accgtggcgc cgagctcccg aaagatctcc agggcttccc ggatcgtctc caccacgggc
     4321 tcgtcgacgc ctttggacac g
//
LOCUS       DBNHOX010000470         5073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_808665, whole genome shotgun sequence.
ACCESSION   DBNHOX010000470 DBNHOX010000000
VERSION     DBNHOX010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_808665"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4246
                     /locus_tag="AC16UZ_10425"
     CDS             <1..4246
                     /locus_tag="AC16UZ_10425"
                     /inference="COORDINATES: protein motif:HMM:NF014805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6247140.1"
                     /translation="MILSVRDGVFSTIQPKSLANIARRLNLSWQECAKYVGLSPYELY
                     AQGNIDGVIDYVPDDDVANLRLAIVSGVQQVEEKVKEFVAENPYILDHYRQSLQRYLN
                     PSEQLKVMQASASMKLTKNPTEYLNVFGVAYRYLRYLKVRQRIKATSTHQYGRLAVRE
                     LPQGELTQRAELERRMLFLKWLQDPDRVVYDDALSRAWKNYSDKKQAVHDERGRLAQF
                     LFGEPKKNYEDARALLISSVGVFLYNRWKSESLGNLRALKEMLTRHEDIRHILTVSDL
                     PDARGLIKALRSDYALLPRLREGFTHEGKKLIGEERLEDKSDLYLSSQLTAELNLVIT
                     GPLVAPEAGSPRSSDMARNRAYLVERYPGLVMATAKEGAPVPFSDMTVLDVLVSDDLR
                     RDFVTECENLLLFDSVYDQIIANLDSIAEEAQSRQALAKTSLAQLIDTTLGLAATGVT
                     LGGRGSGEPAETSETAKLRRQFIDWYLRIARMPKGADFFRAVEEWKKAVFPHLSDTLF
                     VVVTHLFDSLLLSFVQSEREGKAYNGRIAPKNIGRRKDFWNRLNIAYRDLLIQNVLRD
                     AKNRRTTGYKAFVDTFFSAFDERDADLLSSDPCEFPGFRLSIEEALNRGIPPCGVVTG
                     IGNFKTGDGDVRVGTVISNVDFQAGSFDMASAEKFCRLLVECAEQHLPVVCFISSGGM
                     QTKEGAGSLFSMAAVNDRITRFVRDHDLPVIVFGFGDCTGGAQASFVTHPLVQTYYLS
                     GTSMPFAGQIVVPSNLPLNSILSNYLSVVAGAMQGLVQHPFLPELDKELRHIDPEIPV
                     PQESVIDVVARVLSGVFSQQRPVVVAHRPKYTEVDLFRPVRRVLIHARGCTAAKLIRI
                     AQRNGIEVVLVQSDPDMESAAVDMLGEKDSVVCIGGNTPDESYLNAMSVLSVAEHEGT
                     DALHPGIGFLSENSQFAELVRSHGINFIGPPVSSMETMGNKSNAINTALRLNVPVVPG
                     SHGILTNVERAAEVAEEIGYPVLIKAVHGGGGKGIQVVESRDSFHELFHRVTVEARAA
                     FGNGDVYLEKYVTSLRHIEAQLLRDVHGNTRVLGIRDCSVQRDKQKVIEESDSTVLPD
                     RLREEVLQHTAALADEVGYVGAGTVEFIYDLKSDAVYFMEMNTRLQVEHPVTEWVSGV
                     DIVSQQFRIAAGESIADLDPSPRGYSIEARVTAERVQAAPGGELVFRPQPGQVAECSF
                     PEDEGVEIIAAAAPGKFISPYYDSMIAQVVVYADDRAQAIEKLAAYLRRVRVTGICTN
                     IPLLKHILDDEVFQQGVYDTGYLPGLLARLDADELIAEIEGGAGEVSGGIDMNAIRID
                     ESDELKVLSPATAIFYGTPTPTEPPYVSVGDRVKLSDTLCQLEAMKIFTPLSLADFNS
                     EFELYEAGREYEITRINMGTGQQVNAGDLLFVVRPV"
     gene            complement(4265..>5073)
                     /locus_tag="AC16UZ_10430"
     CDS             complement(4265..>5073)
                     /locus_tag="AC16UZ_10430"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR6247141.1"
                     /translation="SYSIHSLFSWLWLGHPEHILPGVIMWSTLLLASVFATSVLSGVL
                     GMAGGMILMAILVSLMSVPAAMIIHGVVQATSNGSRAWFLRAHIRWRVLPPYLLGAAV
                     TLAAFAALTLVPEAGLVLVLVGIFPWLARVVPRLQGLDVERPATAVACGATVTAAQLF
                     AGASGPLLDVFYLNSSMDRYQVIASKAFTQTIGHGLKLAYYGTIIGVAETLPWWMFGA
                     AMATAVAGTRVGTRLLGRLRDDVFRRVSGWVILAIAAVCVVKGGMELAGW"
BASE COUNT          965 a         1617 c         1608 g          883 t
ORIGIN      
        1 catgatcctg tcggtccgcg acggggtctt cagcaccatc cagccgaaaa gcctggccaa
       61 catcgcgcgt cggctgaacc tgtcctggca ggagtgcgcc aagtacgttg gcctgtcgcc
      121 ctacgagctc tatgcccagg gcaacatcga cggggtcatc gactatgtgc ccgacgacga
      181 cgtggccaac ctccgcctgg ccatcgtgtc cggggtgcag caggtcgaag agaaggtcaa
      241 ggagttcgtc gcggagaacc cctacatcct cgaccactac cggcagagcc tgcagcgcta
      301 cctgaaccct tcggagcagc tgaaggtcat gcaggccagc gcgtcgatga agctgacgaa
      361 gaaccccacg gagtatctca acgtcttcgg cgtggcgtac cgctatctgc gttacctcaa
      421 ggttcgacag cgtatcaagg ccaccagcac ccaccagtac gggcgtctgg cggtgaggga
      481 gctcccccag ggcgagctca cccagcgggc ggagctcgaa cggcgaatgc tgttcctcaa
      541 atggctgcag gatccggacc gggtcgtcta cgacgatgcc ctttcccgtg cctggaagaa
      601 ctacagcgac aagaagcagg cggtgcacga cgagcgcggc cggcttgctc aatttctctt
      661 cggtgagccc aagaagaact acgaggatgc ccgggcgctg ttgatctcct ccgtgggcgt
      721 cttcctctac aaccgctgga agtccgagtc ccttggcaac ctgcgcgctc tcaaggagat
      781 gctcacacgt cacgaggaca ttcgacacat cctaacggtc agcgacctgc ccgacgcccg
      841 cggcctgatc aaggcgctgc ggtccgacta cgcgctgctg ccgcgcctgc gtgaaggatt
      901 cacccacgaa ggcaagaagc tcatcggcga ggaacgcctc gaggacaaat cggatctgta
      961 tttgagctca cagctcaccg ccgagctgaa cctggtcatc accgggccgc tggtagcccc
     1021 ggaggcaggg agcccgagat catcggacat ggcgcgcaac cgcgcctacc tggtggagcg
     1081 ctatccgggc ctggtgatgg caacggcaaa ggaaggtgcg ccggtgccct tcagcgacat
     1141 gaccgtgctg gacgtgctcg tctcggacga tctccggcgc gacttcgtga cggagtgcga
     1201 aaatctcctg ctgttcgact ccgtctatga ccagatcatc gccaacctgg attccatagc
     1261 cgaggaggcc cagtctcggc aggcgctggc caagacatcg cttgcgcagc tcatcgacac
     1321 caccctcggc cttgccgcta ccggagtcac cctggggggc agaggcagtg gcgagccggc
     1381 ggagacgtcc gagacggcga agcttcgccg gcagttcatc gactggtatc tgcgcatcgc
     1441 gcggatgccc aagggtgccg atttcttcag ggcggtcgag gaatggaaga aggcggtctt
     1501 tccgcacctc tctgacaccc tgttcgtggt ggtcacgcac ctcttcgaca gcctgctgct
     1561 ttccttcgtg cagtcggaac gcgagggcaa ggcttacaac gggcggatcg cgccgaagaa
     1621 catcgggcgg cgcaaggatt tctggaaccg tctgaacatt gcgtaccgtg accttctcat
     1681 ccagaacgtg ctgcgcgatg ccaagaaccg ccgcacgacg ggttacaagg ccttcgtcga
     1741 cacgttcttc tccgccttcg acgagagaga cgccgatctt ctgagctcgg atccctgcga
     1801 gtttccggga ttccggttgt ccatcgagga agccctgaat cgcggcatcc cgccctgcgg
     1861 cgtggttacc ggcatcggta acttcaagac cggcgacgga gacgtccggg tgggaacggt
     1921 gatttccaac gtggatttcc aggccggctc cttcgacatg gccagtgccg agaaattctg
     1981 ccggctgctc gtggagtgcg ccgagcagca cttgccggtg gtctgcttca tctcctcggg
     2041 cggcatgcag acgaaggagg gcgcgggctc gcttttctcc atggccgccg tcaacgaccg
     2101 catcacgaga ttcgtccgcg accacgacct gccggtcatc gtgttcgggt tcggcgactg
     2161 cacgggggga gcccaggcga gtttcgtcac gcatcccctg gtgcagacct actacctttc
     2221 agggaccagc atgccgttcg ccggccagat cgtggtgccg tcgaacctgc cgctcaattc
     2281 gatcctctcc aactatcttt ccgtggtcgc cggcgccatg caggggctgg tccagcaccc
     2341 gtttcttccg gagctcgata aggagctgcg gcacatcgat cctgagatac ccgttcccca
     2401 ggagtccgtc atcgacgtgg tcgcgcgcgt gctctcgggc gtgttcagcc agcagcgtcc
     2461 ggtggtggtg gcgcaccggc ccaagtacac ggaggtcgat cttttccggc ccgtgcgccg
     2521 tgtgctcatt catgcccgcg ggtgcacggc cgccaagctg atccgtattg cccagcgtaa
     2581 cggcatcgag gtcgtgctgg tgcagtcgga cccggacatg gaatccgccg cggtggacat
     2641 gctgggcgaa aaggactcgg tggtctgcat tggtggcaac acccccgacg agagctacct
     2701 gaacgccatg agcgtcctga gcgtggccga gcatgagggc accgacgcgc tgcatccggg
     2761 catcggtttt ctttccgaga actcccagtt cgccgagctg gtgcgttccc acgggatcaa
     2821 cttcatcggg cctccggtgt ccagcatgga gaccatgggc aacaagtcca atgccatcaa
     2881 caccgcgctg cgcctaaacg tgccggtggt cccgggaagc cacgggatcc tcaccaacgt
     2941 cgagagggcg gccgaggtgg cggaggagat cggatacccc gtgctcatca aggccgtgca
     3001 tggcggaggc ggtaagggca tccaggtggt ggagtcgcgc gattctttcc acgagctctt
     3061 ccaccgggtc acggtagaag cgcgcgcggc gtttggaaac ggcgatgttt acctggagaa
     3121 gtacgtcacc tctctaaggc acatcgaggc ccagctgctc agggacgtgc acggcaatac
     3181 ccgcgtgctc ggcatccgcg actgcagcgt tcagcgcgac aagcagaagg tgatcgagga
     3241 gtccgactcc acggtgttgc ccgatcgcct tcgcgaggag gtcctgcagc acacggcggc
     3301 cctggcggac gaagtggggt atgtgggtgc aggcaccgtc gagttcatct acgacctgaa
     3361 gtccgatgcc gtgtatttca tggagatgaa cactcggttg caggttgagc atccggtgac
     3421 ggagtgggtc tccggcgtcg acatcgtttc ccagcagttt cgcattgccg ccggggaatc
     3481 catagcggat ctcgatccgt caccccgcgg gtactccatc gaggcgcgcg tgacggccga
     3541 gcgcgtccag gccgctcccg gcggcgagct ggtcttccgc ccgcagcccg ggcaggtggc
     3601 cgagtgcagc tttccggagg acgaaggggt cgagatcatc gccgctgcag cgcccggcaa
     3661 gttcatctcc ccctactacg acagcatgat tgctcaggtg gtcgtctacg cggatgaccg
     3721 tgcccaggcc attgagaagc tggcggccta tctgcgccgc gtcagggtta cggggatctg
     3781 caccaacatt cccctgctca agcacatcct cgatgacgag gtctttcagc agggcgtgta
     3841 tgacacgggc tacctgccgg ggcttctcgc ccgcctggat gccgacgagc tcatcgcgga
     3901 aatcgagggc ggtgccgggg aggtttccgg cggcatcgac atgaatgcca tacgcatcga
     3961 cgaaagcgac gagctcaagg tgctctcgcc cgcgacggcc attttctacg gaacgcccac
     4021 gcccaccgag ccgccctacg tgtcggttgg ggaccgggta aagctgtcgg acaccctctg
     4081 tcagctcgaa gccatgaaga tattcacgcc cctttcgctg gccgatttca actccgagtt
     4141 cgaactctat gaagcgggcc gggagtacga gatcacccga atcaacatgg gcaccggaca
     4201 acaggtgaac gcgggagatc tgctgttcgt ggtgcggccc gtttagggag ggcacgggca
     4261 gcgctcacca gccggccagt tccatcccgc ccttgacgac gcagacggcg gcgattgcca
     4321 ggatcaccca tccactcacg cggcggaaca catcatctcg caggcggccc agcagccggg
     4381 ttccgacgcg cgtgccggcc acggccgtcg ccatggcggc gccgaacatc caccagggca
     4441 gggtctcggc aacgccgatg atggtcccgt agtaagcgag cttcagcccg tggccgatgg
     4501 tctgagtgaa cgccttgctg gcgatcacct ggtagcggtc catggacgag ttcaggtaga
     4561 agacgtccag cagcggtccc gatgccccgg cgaatagttg cgccgcggta accgttgcac
     4621 cgcatgccac cgccgtcgcc ggcctttcca cgtccaggcc ctggagtcgc gggacgacgc
     4681 gagccagcca gggaaaaatc ccgacgagaa cgagcaccag gcctgcttcg gggacaagcg
     4741 tcagggctgc aaaagcggca agggtgacgg ccgcgcccag cagataaggg ggcagcaccc
     4801 gccaccggat gtgtgcgcgc agaaaccagg ccctggaccc gttcgaggtg gcctgaacca
     4861 cgccgtgaat gatcatggcg gcgggcacgg acatcaggga gaccaggatg gccatcagga
     4921 tcatgccgcc cgccatcccc agcacgccgg agagcacgct ggttgcgaag acgctcgcga
     4981 gcagaagggt cgaccacatg atcacgcccg gcaaaatgtg ctcagggtgc cccaaccata
     5041 accatgaaaa aagagaatgg atggaatatg atc
//
LOCUS       DBNHOX010000471         1713 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_102087, whole genome shotgun sequence.
ACCESSION   DBNHOX010000471 DBNHOX010000000
VERSION     DBNHOX010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_102087"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="AC16UZ_10435"
     CDS             <1..326
                     /locus_tag="AC16UZ_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR6247142.1"
                     /translation="INTAGEKVYPEEVEEALKHHESVADALVVGLPDERWGQAITAVI
                     TLHPGYSLDEIGLREFARRELAGYKLPKRILAKDDLERAPNGKANYAAIREFAQAELG
                     ISSGS"
     gene            462..818
                     /locus_tag="AC16UZ_10440"
     CDS             462..818
                     /locus_tag="AC16UZ_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247143.1"
                     /translation="MSERDEQEKLEEQACALLLRSPHLSVSQVLDLMDIGDSEFREML
                     RSNPRIASLMEQRREGALPRVESKMTECPACGEWFIPYASARCCSDTCAKIDQISNRP
                     NAARRFRLRNRQTGGS"
     gene            complement(843..1124)
                     /locus_tag="AC16UZ_10445"
     CDS             complement(843..1124)
                     /locus_tag="AC16UZ_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247144.1"
                     /translation="MISLTFYGLACLCGIAAVLILLVGFVDWLQTERWTNLSVLEFGY
                     DAHLIRARWFINNRWSWWLHDLMHLVPLYVFLLALAPLSWVVGAWFGRR"
     gene            1336..>1713
                     /locus_tag="AC16UZ_10450"
     CDS             1336..>1713
                     /locus_tag="AC16UZ_10450"
                     /inference="COORDINATES: protein motif:HMM:NF020856.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX-like domain-containing protein"
                     /protein_id="HIR6247145.1"
                     /translation="MSHVHIFAGNPLDRADALRRDPEWLDGARADPGSRYLPFARLNV
                     LLCGERPELGWLRSSEVQRLGISVPPIFLGLREGIAHFAIDVSELGDPAHELNLDDSW
                     RFEDCRAAGTVLSVPDSGIVAQSR"
BASE COUNT          341 a          537 c          551 g          284 t
ORIGIN      
        1 gcatcaacac cgccggggag aaggtatatc cggaggaagt cgaggaggcg ctgaagcatc
       61 acgagtccgt ggcggatgcc ctggtggtgg gactccccga cgagcgctgg ggccaggcga
      121 tcaccgccgt gatcacgctc catccgggct actccctgga cgagatcggt ctgcgcgagt
      181 tcgcccgtcg ggaactcgcc ggttacaagt tgcccaagcg cattctggcc aaggacgacc
      241 tcgaacgcgc acccaacggc aaggcaaact atgcggccat ccgggaattt gctcaggcgg
      301 aactgggcat ttcatcgggt tcgtaatacc gtctggagga cgggccgtag gcgatcgtca
      361 tttacccagg gcaacattgc gcggcgacat gagcgcacga ctggccgtga cccggccggc
      421 cgacaccgat tgagtcatct gccggcagga gtattggccg cgtgagcgaa agggatgagc
      481 aggagaaact cgaggagcag gcgtgcgccc tcctgctgcg gtctccgcat ctcagcgtca
      541 gccaggttct cgacctgatg gacatcggcg actccgaatt tcgcgaaatg ctcaggagca
      601 atccgcgcat cgcctcgctc atggagcagc gtcgggaggg cgctctgccg agggtggagt
      661 ccaagatgac ggagtgcccc gcctgcggag agtggttcat tccctacgcg tctgcgcgct
      721 gctgctcgga tacctgcgcc aagatcgacc agatcagcaa caggcccaac gcggcgcgac
      781 gctttcgtct gcgcaaccgg cagaccggcg gctcctgacg tcgaccacgc ccagtcgggg
      841 gttcagcgcc gaccgaacca ggctcccacc acccaggaca ggggggccag agcaagaaga
      901 aacacataga gcgggaccag gtgcatcagg tcgtggagcc accagctcca gcggttgttg
      961 atgaaccagc gcgccctgat gagatgagcg tcgtagccga actcaaggac gcttagattg
     1021 gtccagcgct ctgtctgcag ccagtcgacg aaaccgacca gaagaatcag caccgccgcg
     1081 atcccgcaga gacaagcgag cccgtagaaa gtcaaggaaa tcatgcgatc agccatggtg
     1141 ccagtcgatc cgtctgaaga tgaaccattg acccaagcgt agagttccgg atgcgcgaag
     1201 gcgatgccgt gacggctttc acgaatcccg ggaagccgaa cccgttccag aacggctcga
     1261 ggacggcggt ttcacaatgc gcgcaaggtc tttacccttc tcctttcatc cacctgaccc
     1321 ttacctggaa gatccgtgag ccacgttcat atctttgctg gcaatcccct ggaccgcgcg
     1381 gacgcgctgc ggcgcgatcc cgagtggctc gacggcgcga gagccgatcc tggctcgcgc
     1441 tatctgccct ttgcaaggct gaacgtgctg ctgtgcgggg agcggccgga gctcggctgg
     1501 ctgaggtcga gcgaggtgca acggctgggc atcagcgtgc cccccatctt cctgggcttg
     1561 cgggagggca ttgcccactt cgcgatcgac gtctccgagc tcggagaccc ggcccacgag
     1621 ctgaacctgg acgattcctg gcgtttcgag gactgccggg ccgcaggcac tgtgctgtcc
     1681 gtcccggaca gcggcatcgt ggctcagagt cga
//
LOCUS       DBNHOX010000472         1631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_359242, whole genome shotgun sequence.
ACCESSION   DBNHOX010000472 DBNHOX010000000
VERSION     DBNHOX010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_359242"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..81)
                     /locus_tag="AC16UZ_10455"
     CDS             complement(<1..81)
                     /locus_tag="AC16UZ_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011336008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR6247146.1"
                     /translation="MANSPQARKRARQAEKRRRQNASQRSM"
     gene            141..>1631
                     /gene="murJ"
                     /locus_tag="AC16UZ_10460"
     CDS             141..>1631
                     /gene="murJ"
                     /locus_tag="AC16UZ_10460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR6247147.1"
                     /translation="MSTGTEKPEQQAAALEGPQNVAAQGGIVSGMTLLSRITGLVRDM
                     ALSYFFGAQDVADAFFVAFRIPNFFRRLFAEGAFSQAFVPVLARYRGGGVGELRRFVA
                     VMQGNLIAALSVFVLLGVIFSPALVAVFAPGFFGDDERFALTSSMVRITFPYLGLISL
                     TAFAGALLNSHHRYAVPAFTPVLLNLSLIAAVLFAADVFATPVFALAWGVLVAGFAQL
                     AFQGPSLRRLGLLVAPRISRTDPGARQVGKLLIPAVLAASVNQINALIDTMLASTLVT
                     GSISWLYYSDRLLELPIGLVAVALGTVLLTTLSRLDSSGDRAGFAATVDWGMRMGAIL
                     GVPAAAALYVLAVPLVASIFLRGALTPLDARMASLSLQAFAVGLPALVLVKVLAPAYF
                     ARRDTATPFRIGAVAVGVNVALNLALFFWMGHVGLALATSASAWVNALMLLRGLVSGD
                     AYRPGRPFLVSTARALAASAVMVAAFAYWVPEPSAWMAAPDGERVVW"
BASE COUNT          184 a          546 c          547 g          354 t
ORIGIN      
        1 catggaacgc tggctggcat tttgtcgccg ccgtttctcg gcctggcgtg cgcgcttgcg
       61 tgcttgtgga ctgttggcca agcgtggctc ctgggggtcc ggattgcgaa ggcgcggtaa
      121 tatgccgccg aggtatggtg atgtcaaccg gcactgagaa acccgagcag caggccgcgg
      181 ccttggaagg tccccagaac gtggccgcac agggaggcat cgtttcgggc atgaccctgc
      241 tgtcccgaat caccgggctg gttcgcgaca tggcattgtc ctactttttc ggggcccagg
      301 acgtcgcgga tgcgttcttc gttgccttcc gcatccccaa ctttttccgt cggctcttcg
      361 ccgaaggcgc gttcagccag gcgttcgtgc cggttctggc caggtacaga gggggtggcg
      421 taggcgagct gcgacgcttc gtcgcggtga tgcaggggaa cctcatagcc gccttgtcag
      481 tctttgtgct gctgggcgtg attttctcgc ccgcgctggt cgccgtgttc gcgcccgggt
      541 tcttcggcga cgacgaacgc ttcgcgctca ccagcagcat ggttcgcatc acgttcccct
      601 atctcggcct catctcgctc accgcattcg ccggggcgct cctcaacagc caccaccgct
      661 acgccgtgcc ggcgttcacg cccgtcctgc tcaacctgag cctcattgcc gcggtgctct
      721 ttgctgccga cgtcttcgcg acccccgtgt tcgccctggc atggggtgtt ctggtcgccg
      781 ggttcgctca gctcgccttt cagggcccct cgctcagaag gctcggactg ctcgtggcgc
      841 cccggatcag ccggaccgac cccggggccc ggcaggtcgg caagctgctc attccggcag
      901 tgctcgcggc gtcggtcaat cagatcaacg cgctcatcga taccatgctc gcctcgacgc
      961 ttgttacggg gagcatctcg tggctgtact actccgaccg gctgctcgag ctccctatcg
     1021 ggctggtggc cgttgctctg ggcacggtgc tcctgacgac gctgtcgcgc ctggattcct
     1081 caggcgaccg ggccgggttc gccgcgaccg tggactgggg catgcgaatg ggggcgattc
     1141 tcggcgtgcc ggcggcagcc gcgctttacg tgcttgcggt tccgctggtt gcgagcattt
     1201 ttctgcgcgg cgcgttgacc cctctcgatg cccgcatggc ctcgctcagc ctgcaggcgt
     1261 ttgccgtcgg cctgccggcg ctcgtgctcg tcaaagtgct ggctccggcg tacttcgcgc
     1321 gccgggacac cgccacgccg ttccgaatcg gcgcggtcgc cgtgggcgtc aacgtggcgc
     1381 tgaatctggc gctcttcttc tggatggggc acgtgggcct ggcgctggcg acgtcggctt
     1441 ccgcgtgggt taatgcgctg atgctgctgc gcgggctcgt ctccggtgac gcttaccgcc
     1501 cgggccggcc gttcctggtg agtacagcaa gggctctggc cgcgtcggcc gtcatggtgg
     1561 ccgctttcgc ctactgggtt ccggagcctt cggcctggat ggccgcgccc gatggcgagc
     1621 gcgtcgtctg g
//
LOCUS       DBNHOX010000473         2162 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1644490, whole genome shotgun sequence.
ACCESSION   DBNHOX010000473 DBNHOX010000000
VERSION     DBNHOX010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1644490"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="AC16UZ_10465"
     CDS             complement(<1..1383)
                     /locus_tag="AC16UZ_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR6247148.1"
                     /translation="MTTDGAVTAGERSHLTFCRICESLCGLRVSTRDGVVTDVRPDGD
                     HVATGGFACPKGLRQHHLYGSPDRLLRPRKRVGGDWRDVSWNEALGDIGGELARIRDE
                     HGPDAIAMYVGTAAGFGVLHPVFAQGFMDGLGSRNMYSSATQDCANKFAVAERIYGFP
                     FTQPFPDLLNTKLLVIVGANPMVSKWSFLQVPNPRRHLNAIRERGGRVVVVDPRRTET
                     AKAAGEHVFIRPNSDLYFYLAFLHELIAAGGVREDRVDAHMNGFEEVRELVEPWTAER
                     AAQHTGVSAEVLRQLVADYVAADGAALYSSTGVNMGAHGSLCFWIQEVINAVSGNLDA
                     RGGTLVGNGIFDFARFGKKYGLLVKDDRSRVGGFRKTNDAFPGGILADEILTPGPGQI
                     RALIVTGGNPLLTMADSERLKQAFGELELLVTLDILPTETGAAAHYMLPCTSPLERPD
                     LPFVFPLVLGM"
     gene            complement(1400..1924)
                     /locus_tag="AC16UZ_10470"
     CDS             complement(1400..1924)
                     /locus_tag="AC16UZ_10470"
                     /inference="COORDINATES: protein motif:HMM:NF025050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6247149.1"
                     /translation="MLDKPTDRSIFQNAWVVDDVEAACMKWVEQMGVGPFFVNEYDNA
                     FEQIIYRGEPAKLDMIVAIAQAGPVQVEIIQPKVDRCAYRDSVPAGTDAFHHMCVWTD
                     DIDADSEYFESLGYPTANRGAVMDIEFAYYDTRPLMGCMLEVVTRGPGIIERFAQIAK
                     AAENWDGKDPIRYD"
BASE COUNT          383 a          704 c          710 g          365 t
ORIGIN      
        1 catcccgagc accagcggga acacgaacgg cagatccgga cgttccaggg gggacgtgca
       61 gggaagcatg taatgagcgg ccgcgccggt ctcggtgggc aggatatcca gcgtcaccag
      121 cagctcgagc tcgccaaagg cctgcttgag gcgttcgctg tcggccatgg tgagcagggg
      181 attgccgccc gtgacgatca gcgcccggat ctgcccgggg ccgggcgtca gaatctcgtc
      241 ggcgagaata ccgccgggaa aggcatcgtt cgtcttgcga aagccaccga cgcgcgagcg
      301 gtcgtccttc accagcagac cgtacttctt gccgaaccgg gcgaagtcga agatgccgtt
      361 gccaacgagg gtgccgccgc gcgcgtcgag gttgccgctg accgcgttga taacctcctg
      421 gatccagaag cacagggaac cgtgcgctcc catgttgacg ccggtggacg agtaaagggc
      481 ggccccatcg gcggcaacgt agtcggcgac gagctggcga agcacttccg cgctgacgcc
      541 agtgtgttgc gccgcacgct cggccgtcca gggctcgacc agctcgcgca cctcttcgaa
      601 cccgttcatg tgagcgtcga cccgatcctc gcgcaccccg cccgccgcga tcagctcatg
      661 gaggaaggcg aggtagaagt agagatcgct gttgggccgg atgaagacgt gctcgcctgc
      721 cgctttggcc gtctccgtgc gccgcggatc gaccaccacg accctgccgc cgcgctcccg
      781 gatggcgttg aggtgccggc gcgggttggg gacctgcaga aagctccact tggaaaccat
      841 gggattcgcg ccgacgatca cgagcagctt cgtgttgagc agatccggga acggctgcgt
      901 gaacggaaag ccgtatatgc gctcggccac cgcgaacttg ttggcgcagt cctgggtcgc
      961 ggaggagtac atgttccgcg agcccaaccc atccatgaag ccctgggcaa atacggggtg
     1021 aagcacgccg aaccccgccg cggtgcccac gtacatggcg atggcatcgg gcccgtgctc
     1081 gtcccgaatg cgcgcgagct cgccgccgat gtcgccgagg gcctcgttcc acgagacgtc
     1141 gcgccagtcg ccgcccaccc tcttgcgagg gcgcagcaaa cggtcggggg agccgtatag
     1201 gtgatgctgg cgaaggccct tcgggcaggc gaacccgccg gttgccacat gatcgccgtc
     1261 cggacggacg tccgtgacca cgccatcccg ggtgctcaca cgcaatccgc acagcgactc
     1321 gcaaatgcgg cagaaggtga ggtgcgagcg ctcgccggcg gtaaccgccc cgtctgtggt
     1381 catgagtcag cgctgccggt cagtcgtagc ggatcgggtc cttgccgtcc cagttctcgg
     1441 cggccttcgc gatctgcgcg aagcgctcga tgattcccgg tccccgcgtg accacctcga
     1501 gcatgcagcc catcaacggc cgcgtatcgt agtaggcgaa ctcgatgtcc attacggcgc
     1561 cgcgattggc tgtcgggtag ccgagactct cgaagtactc ggagtccgcg tcgatgtcgt
     1621 cggtccagac gcacatatgg tgaaacgcgt cggtcccggc aggcacgctg tcgcgatagg
     1681 cgcagcggtc gaccttgggc tggataattt ccacctgcac cgggcccgcc tgggcgatgg
     1741 cgacgatcat gtcgagcttg gccggctctc cccggtagat gatctgctcg aaggcgttgt
     1801 cgtactcgtt gacgaaaaac gggccgacgc ccatctgctc cacccatttc atgcaggcgg
     1861 cttcgacgtc gtcgaccacc cacgcattct gaaaaatgct gcggtccgtt ggcttgtcca
     1921 gcatctcctc cctccagatt cgagtgcgcg ctgagagtag cacatcgaag cgccgcacgc
     1981 ggcggccaag cgcgggggca cgtcatcgtg aaccaccgct gcagcgccag gtttgaaact
     2041 gccggcgtac gtagtagctt gggctcaatt gaacgggcaa tcctcgagct gaggaggacg
     2101 ggttccatga tcgacgtcgt tcgcacgccg gaagaacgtt tcagggggct ccccgacttt
     2161 cc
//
LOCUS       DBNHOX010000474         4627 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_680438, whole genome shotgun sequence.
ACCESSION   DBNHOX010000474 DBNHOX010000000
VERSION     DBNHOX010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_680438"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1379
                     /locus_tag="AC16UZ_10475"
     CDS             159..1379
                     /locus_tag="AC16UZ_10475"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6247150.1"
                     /translation="MAAPLEDITVVAIESWMAAPSASALLADLGANVIKVEPPSGDPM
                     RNLSRAPKVDGPLKALDCQFDVDNRGKRSVAVDLDTDQGKAVVHRLVARADVFMCNLL
                     IHRQERFGLDPDRVLALNPRIVHATLTGYGTTGPDAWRPGYDVTAFFGRSGLYDASRE
                     GDDGDVPMARPAQGDHTTGLALLGAILAALRSAERTGEGQVVETSLFETAIWTQASDF
                     GTTAIDRAPVRRRARHNMLSPTANRYPCGDGKWLVVNMLDAMAFDRFCRAIGREDWLE
                     DERFREARGRYDHMPELVTGIDATLATRSRDEWGRIFDEAGLIWGPVLTLDEAAGDGQ
                     AHAIGMFPELEHPEYGTYRTVNAPFRFRSGEVRPRGTSPGPGEHTREVLGEMDFDEAE
                     IDALMAAGVLPTHD"
     gene            complement(1376..1690)
                     /locus_tag="AC16UZ_10480"
     CDS             complement(1376..1690)
                     /locus_tag="AC16UZ_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247151.1"
                     /translation="MNHPVTQRSLRQQNQAFSGTNGVSQNNRSMRFLPAFRDEETGQV
                     ELARFDDGRVAPAHLIVGLPRRWAVAFASDGAILALKPSITAGFVRDDVFFTREEAAS
                     AD"
     gene            complement(2272..2922)
                     /locus_tag="AC16UZ_10485"
     CDS             complement(2272..2922)
                     /locus_tag="AC16UZ_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247152.1"
                     /translation="MTRPAAPRLPSGLLLALLLAPQVASGHGGVVLEEDQCVLKMDFL
                     LAHFTGYQPESRGSEEFCEDIPEVGESLFVLEYMHDFMKEMPVDFRIISDDTELGTFA
                     NWDDVHALGDLTPRTVFYQPPRKQADGVLTASHRFDQAGGYIGIVTASHPTQEKIYNA
                     VFFFRVGGPDLGYLPLFVGLIILVQLIYWGATGTLQRFLRRYLPNRRNYGEAAAAG"
     gene            3020..4003
                     /locus_tag="AC16UZ_10490"
     CDS             3020..4003
                     /locus_tag="AC16UZ_10490"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR6247153.1"
                     /translation="MATIAILGLGNFGTALARVWLLGGHDVRGWTVEEDVFDAIRSKG
                     SNSKYLDGIDLTGLQVSMELGESVPGAELVVLALPSHVVLHVADDVIPLLTPDQVVLD
                     LAKGIAGEELVSDAIQAKLAAAGKTNPLAVITGPTIAPELASGVLTTALVASEDESLA
                     SRLAEQLSTSTFSLKAASDPRGAELWGGFKNVIALGCGIADGLDEGSGDNLKAAIFTA
                     GFAEGNRLLTALGAAPETAFTPAGIGDLFVTASSPHGRNRSLGQKLGAGQTLEEAMAG
                     SVMVAEGVRATRMFAELAAARSVTTPFIAALTDLLDGNCTAPDCVRRLMAI"
     gene            4099..4398
                     /locus_tag="AC16UZ_10495"
     CDS             4099..4398
                     /locus_tag="AC16UZ_10495"
                     /inference="COORDINATES: protein motif:HMM:NF018717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR6247154.1"
                     /translation="MAAFFVAQYTVNNPALYAEYQQGAGPTLATYGAELVSFDVGAET
                     VEGNPPGPQTVIIKFESTDAAKAWYNSAEYQAVVGKRLEATDGFAVIAQSMNAGG"
     gene            complement(4432..>4627)
                     /locus_tag="AC16UZ_10500"
     CDS             complement(4432..>4627)
                     /locus_tag="AC16UZ_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247155.1"
                     /translation="GLGLGPQVTGILSDGFNAFGDLGTDSLRYALICVLLLNVVATVY
                     YWMAGRDLERDLARRGELDG"
BASE COUNT          828 a         1542 c         1500 g          757 t
ORIGIN      
        1 cgcccacggc cctcgcagat agtgcagttc ccgcatggcg cgcaaggtta ccgcgccgcc
       61 gaagctactg ccagttctcg cgcggaagct gaatttgccg cgaatatgcg gtacaaccgg
      121 gctttcacgc cccgtgagct caaagaggag gcgagccgtt ggctgcaccg ctggaagata
      181 tcaccgtcgt cgccatcgag agctggatgg cggcgccgag cgcgagcgcc ctgctggcag
      241 acctcggcgc aaacgtgatc aaggtcgagc cgccgagcgg cgatcccatg cgcaatctct
      301 cgcgcgcgcc caaggtcgac ggtccgctca aagcgctgga ctgtcagttc gacgtggaca
      361 accgcggcaa gcgatcggtg gccgtggacc tggacaccga ccagggcaag gccgtcgtgc
      421 atcgcctcgt cgcccgggcc gacgtgttca tgtgcaacct gctcattcat cggcaggaac
      481 gtttcgggct cgatcccgac cgcgtcctgg cgctgaaccc gcgcatcgtg cacgccacgc
      541 tgaccggcta cggcaccacc gggccagacg cctggcgccc cggctacgac gtgaccgcct
      601 tcttcggccg ctcgggtctc tacgatgcca gccgcgaggg cgacgacgga gacgtgccca
      661 tggcgcgccc ggctcagggc gaccacacca ccggcctggc gctgctcggg gccatcctcg
      721 ccgcgttgcg atcggccgag cgcacgggcg agggtcaggt ggtggaaact tctctgttcg
      781 agacggccat ctggacccag gcctcggatt tcggcaccac ggccatcgac cgtgcccccg
      841 tgcgtcggcg tgcgcgtcac aacatgctct ccccgaccgc aaaccgctat ccgtgcggcg
      901 acggcaagtg gctggtggtg aacatgctgg atgccatggc gttcgaccgc ttctgccggg
      961 caatcggcag agaagactgg ctggaggacg agcgtttccg tgaagcccgc ggccgctacg
     1021 accacatgcc cgagctggtt acgggcatag atgcgacctt ggcgacccgc agccgcgatg
     1081 agtggggccg gatcttcgac gaagccggcc tgatctgggg gccggtgctg accctcgacg
     1141 aggccgccgg cgacggccag gctcacgcca tcggcatgtt ccccgagctc gagcacccgg
     1201 agtacggcac ctaccggacg gtcaacgcgc cgttccgatt ccgatcgggg gaggtcaggc
     1261 cgagaggaac gtcaccgggt cccggtgagc acaccagaga agtgctcggc gagatggatt
     1321 tcgacgaggc ggagatcgac gcgttgatgg cggccggggt gttgccgacg cacgactagt
     1381 ccgcagaggc ggcttcctcg cgggtgaaga agacgtcgtc gcggacgaac ccggcggtga
     1441 tgctcggttt gagcgccaga atggcaccgt cgctcgcaaa tgcgaccgcc catcgacgag
     1501 ggagcccgac gatgaggtgc gcgggcgcga ccctgccgtc gtcgaagcgc gccagctcca
     1561 cctgtccggt ttcctcgtcg cggaaggccg ggaggaaacg catcgagcgg ttgttctggc
     1621 tgacaccgtt ggtgccggag aaggcctggt tctgctggcg cagcgacctt tgggtgaccg
     1681 gatggttcat gacgacgtga agctcctaca acttccctga agctccgcac atgccagtcg
     1741 agacctcagc tggaatggca gttgcgtttc agattcgctc attcgttacc taccgtttcg
     1801 tttagcatca accatgccaa actccagaat ttcagccgag gctccataac accctgtttt
     1861 ccatgaacaa atcgtgaacg aaacgccgta gcaggacact ttcgtgtgac gccgcttgtc
     1921 agttgcgcgc ccggctgccc caccgcgccc ggggcgcgat ggcgtgccgc ctgctcgagt
     1981 tcacatgaac ggcccgaacc agttccatcc gacgccagct cgcgcattcg gggcaacggg
     2041 ctaaagtcat cgttccgcgc cacatgcggc gtgggtctga acagcagata cggtgggccg
     2101 acctccgaag atggagatcg atcatggctg cttcggaacc gcgaagatgc cttgcgcgac
     2161 atgcttgcgc gacatggtgg aaaccggctt ctagccgacg acgtacgttt ccgcccgatc
     2221 tccccagctt ccccttgact ggcaggccgt cccgggcacg acgccggatt cctaaccggc
     2281 cgccgccgct tccccgtagt tcctccgatt gggaaggtag cggcggagaa accgctgcaa
     2341 agtgccggtt gccccccagt agatgagctg aacgagaatg atcaaaccga cgaagagggg
     2401 aaggtagccc agatccgggc cacccacgcg gaagaagaac accgcgttgt agatcttctc
     2461 ctgcgtcggg tggcttgccg tgacgatgcc gatgtagccg cccgcctggt cgaaccgatg
     2521 actggcggtc agcaccccgt cggcctgttt ccggggcggc tggtagaaga ccgtgcgcgg
     2581 cgtcagatcg ccaagggcat gcacgtcatc ccagttcgcg aacgtgccga gctccgtgtc
     2641 atcggagatg atgcggaagt ccaccggcat ctccttcatg aagtcgtgca tgtactcgag
     2701 cacgaacagg ctctccccca cctcgggaat gtcctcgcag aattcctcgg atcccctgga
     2761 ttcgggctgg tagccggtga agtgggcgag aaggaagtcc atcttcagaa cgcactggtc
     2821 ctcctcgagc actaccccgc catgacccga tgcgacctgg ggcgccagca gcagggcgag
     2881 cagcagcccg cttgggagac gaggagcggc cggtctggtc attgggagtt tgatgcgttc
     2941 ttcctccggc gtcttgcctg atcggctaac tttggcatac aacaggcaac gatccaacgg
     3001 tgaccaaggg gcggcagaca tggccacgat cgcgattctc gggctcggca acttcggcac
     3061 ggccctggcc cgggtctggc tgctaggcgg gcacgacgtc cgcggatgga cggtggagga
     3121 agacgtgttc gacgccatcc gctccaaagg cagcaacagc aaatacctcg acggcatcga
     3181 cctcaccggc ctgcaggtat ccatggagct tggcgagtcg gtccccgggg cggagctcgt
     3241 ggtgctcgcc ctgccctccc acgtggttct tcacgtcgcc gacgacgtca tacccctgct
     3301 gacgccggat caggtggtgc tggatctcgc caagggcatc gccggcgagg agctcgtttc
     3361 cgatgccatc caggccaagc tggcggccgc cggcaagacc aacccgctcg cggtgatcac
     3421 cggcccaacc atcgcgcccg agcttgccag cggcgtgctc accacggcgc tcgtggccag
     3481 cgaagacgaa tcgctggcga gccgcctcgc cgagcagcta tccacctcga ctttttcgct
     3541 gaaggcggcc agcgacccgc gcggcgcgga gttgtggggg ggcttcaaga acgtgatcgc
     3601 gctgggctgc ggaatcgccg acgggcttga cgagggaagc ggcgacaacc tgaaggcggc
     3661 gatcttcacg gccggttttg ccgaaggtaa tcgcctgctc accgcgctgg gggccgcgcc
     3721 ggagacggcg tttactccgg ccggcatcgg cgatcttttc gtcaccgcat cgtcacccca
     3781 cggccgcaac cggtccctgg gccagaagct gggcgccggg cagaccctgg aggaagccat
     3841 ggccggcagc gtcatggtcg cggagggcgt tcgcgccacg cgaatgtttg ccgagctcgc
     3901 cgcagcccgg agcgtgacca cgccgttcat cgcagcgctt acggacctcc tcgacggaaa
     3961 ctgcaccgcg cccgactgcg tccgccgact gatggcgatc tgaagcagac ggcccctctc
     4021 ggcaacggcc aattgtggct gcggggtaat cgggtaccat agccggtctc atcacatcgc
     4081 aaaggggagg agccaaacat ggccgcattc ttcgtcgctc agtacaccgt caacaacccg
     4141 gccctttacg ccgagtacca gcagggcgcc gggcctactc tggccaccta cggggctgag
     4201 ctggtctctt tcgacgtagg cgccgagacc gtcgagggca acccgcccgg cccgcagacg
     4261 gtgatcatca agttcgaatc cacggacgcc gccaaagcct ggtacaactc ggctgagtat
     4321 caggccgtgg tcggcaagcg cctggaagcc accgacggct ttgccgtcat cgctcagtcg
     4381 atgaacgcgg gcggctgatc gcgcaccgca cggcgcgggg ttgacggccg ctcagccgtc
     4441 cagctcaccg cgccgtgcca gatccctctc caggtcccga cccgccatcc agtagtacac
     4501 ggtggccacg acgttgagca gcagcacgca gatcagcgcg tagcgcagcg agtcggtgcc
     4561 gaggtccccg aaggcgttga acccatcgct gaggatgccc gtgacctggg gacccagccc
     4621 aagaccg
//
LOCUS       DBNHOX010000475         1548 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_423298, whole genome shotgun sequence.
ACCESSION   DBNHOX010000475 DBNHOX010000000
VERSION     DBNHOX010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_423298"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="AC16UZ_10505"
     CDS             complement(<1..862)
                     /locus_tag="AC16UZ_10505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR6247156.1"
                     /translation="MMAILAGMANAPSSRRVLAVSFAVDLFDVASNLVVGLLTGSAVV
                     FSEMAQGLADSTGSALLVIGERRSKRQSDDAHPFGYAREAFFWGLLSAVAMLVIGAGL
                     SAWRGYQQLVNPSPLEAPLLAIAVVGLAVVTNGYAVSLSLRKLVAENGSIGRVITGMS
                     QPLVKSALLRDSIGTFTSIIGLVALALYWWLDLLIFDALGALAAAGMMVAASLVLMVQ
                     ARALITGQSLPEDLLVRVRGEVLSTPGVEAVNRLDAVYSGTSQVLLDADLDLAEHLDT
                     AQIEALLDLVE"
     gene            951..1024
                     /locus_tag="AC16UZ_10510"
     tRNA            951..1024
                     /locus_tag="AC16UZ_10510"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:983..985,aa:Gly,seq:ccc)
     gene            complement(1035..>1548)
                     /locus_tag="AC16UZ_10515"
     CDS             complement(1035..>1548)
                     /locus_tag="AC16UZ_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HIR6247157.1"
                     /translation="VRDEFGLTNVKIMVPFCRRIPEAESVLASMAEEGLVRGQNGLEI
                     YVMCEIPNNVILIDEFAKHFDGFSIGSNDLTQLTLGVDRDSEIVAFDFDERDPGVMKM
                     LELAVLGAKRNARYSGICGQAPSDYPEVAEFLVRLGIDSLSLNPDSVLETTRQVLALE
                     QQADRGKAPR"
BASE COUNT          296 a          541 c          476 g          235 t
ORIGIN      
        1 gctcgacgag atccagcagc gcctcgatct gtgcggtgtc gaggtgctcg gcgaggtcca
       61 ggtccgcatc gagcagcacc tgtgacgtcc ccgagtacac ggcgtcgagc cggttcaccg
      121 cctccacacc cggcgtggag agcacctcac cacgcaccct gaccagcaga tcttcaggca
      181 gcgactgacc ggtgatgagc gcccgggcct gcaccatcag caccagcgac gcggcaacca
      241 tcatgccggc cgcagccagg gcgcccaggg catcgaagat cagcagatcg agccaccagt
      301 acagcgccag cgccacgagg ccgatgatgc tggtaaaagt gccgatcgag tcgcgcagca
      361 gggcgctctt cacgagcggc tggctcatgc cggtgatcac cctgccgatg ctgccgttct
      421 cggcgacgag cttgcgcagg ctgaggctca cggcatagcc gttcgtgacc accgccaggc
      481 ccaccacggc gatcgccagc aacggtgcct cgagcgggga cggattgacg agctgctgat
      541 aaccccgcca ggccgacagg cccgcgccga tcaccagcat ggcgacggca gacagcaatc
      601 cccagaagaa tgcctcccgc gcatagccga agggatgcgc gtcgtcgctc tgccgcttcg
      661 accggcgctc accgatgacg agcagcgccg agccggtgct gtccgccagt ccctgggcca
      721 tttccgagaa caccacggcg ctgccggtca acagcccgac caccaggttg ctcgccacat
      781 cgaacagatc gacggcgaac gagaccgcca gcacgcgacg ggaggaagga gcgttagcca
      841 tgcccgctag gatagccatc atcgtggttc acgccagcgc ggccccgcag gacgccacgc
      901 tcgacggcgc tgttgcgacc gccgggcgaa caggtaaact cgatcgccct gcgggtgtag
      961 ttcaatggta gaacctcagc ttcccaagct gatggcgtgg gttcgattcc catcacccgc
     1021 tccactcaga agcgctagcg gggtgccttg ccccggtcag cctgctgttc cagcgccagc
     1081 acctgcctcg tggtttccaa caccgagtca gggttcagcg acagcgagtc gatccccagg
     1141 cgcaccagaa actccgccac ttccggatag tcggacggcg cctggccgca gatgcccgag
     1201 tagcgtgcgt tgcgtttggc accgagcacc gccagctcca gcatcttcat cacccccgga
     1261 tcccgttcgt cgaagtcgaa ggcaacgatc tccgagtcgc ggtccacgcc gagggtgagc
     1321 tgggtgaggt cgttggaacc gatggagaag ccgtcgaagt gcttggcgaa ctcgtcgatc
     1381 aggatcacgt tgttcgggat ctcgcacatc acgtagatct cgagcccgtt ctgcccgcgc
     1441 acgaggcctt cctccgccat gctggcgagc acggactcgg cctcaggaat ccgccgacag
     1501 aagggcacca tgatcttcac gttcgtgagc ccgaactcgt cccggacg
//
LOCUS       DBNHOX010000476         2678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1580496, whole genome shotgun sequence.
ACCESSION   DBNHOX010000476 DBNHOX010000000
VERSION     DBNHOX010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1580496"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="AC16UZ_10520"
     CDS             complement(<1..1263)
                     /locus_tag="AC16UZ_10520"
                     /inference="COORDINATES: protein motif:HMM:NF025038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6247158.1"
                     /translation="MANELNPEQLLRRVDETGFPFATTADVVALEGPVAQTRAREAVD
                     FGVGIKFPGYNVYALGPAQTDKRALILARLKELAGGQDAPDDWCYLQNFEDPARPISL
                     RLPAGVGRRFRHHVQKFISSVRTMVPAALVSDEYRQKSQQLASSLQQRQSSDAAALEA
                     EARELELAMLPSPNGFIFAPVSDGKVMEEDAFMALDEGERQRIQKAIESMTDRLITRL
                     RDYPQFQQELVRQQRELVEKTAEQVITVLLARVRQRYQVYEEVMRYLARVQNELLANV
                     DKIVALERGQQTPMMPGVDPERFLERFNVNLIVDGSEADGVPVLYESNPSLDNLVGKL
                     EHRMELGTPVTDFNLIRPGALHRANGGYLLLDAERVIQKPFAWEALKRALADRSVRIE
                     SVSQLMSLTYSISLEPDPIPLDVKIVLLG"
     gene            complement(1317..>2678)
                     /locus_tag="AC16UZ_10525"
     CDS             complement(1317..>2678)
                     /locus_tag="AC16UZ_10525"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6247159.1"
                     /translation="RSELGDAAFAVGVRLPNHDYVPGGLTPEMNAEIAQRVDPLVDYV
                     SLHMGAYWRFHKLIAPSDDPLGVEMPSNEPITRVVSKPTIVVGRIMTLDHASHIVDTG
                     AADMVSMVRALIADPELVNKARRRDEHKIRPCIGSNMGCVGQLMTAGRISCVVNIAAA
                     REAKVTHDPETKAAAPKRIMVVGGGPAGLEFARTAAMRGHEVELHEAMKVLGGQVTMA
                     ASAPHRADLGAITSWLAEEIDGLGVTVHLNSMVEPDMIAERAPDEVVIATGTTPRADG
                     FQVSTPAASVPGYDLPHVYSSWDLLGFGDITHIEGPAVVFDDTGSFEAISVADVLLGA
                     GVPVTMVSRLNAIGETLPYPPVTVGAARERLMSGDFDFVGGHYLRSINPQEVEVGVLY
                     TERSRRIAARTVILVSFNAPNREIAEALTAQGHTPHLIGDVRARSSIMSAIHGAAELG
                     RRI"
BASE COUNT          439 a          907 c          862 g          470 t
ORIGIN      
        1 tccgagcaga acgatcttca cgtccagagg gatcggatcc ggctccaacg agatggaata
       61 ggtcaggctc atcagctggc tcaccgattc gatccgtaca gagcggtcgg ccagcgctcg
      121 cttgagcgct tcccaggcga acggcttctg gatcacgcgt tcggcatcca gcagcaggta
      181 gcccccgttg gcccggtgga gcgcgcccgg gcggatcagg ttgaaatccg tcaccggcgt
      241 cccgagctcc atccggtgct cgagcttgcc cacgaggttg tcgaggctcg gattgctctc
      301 gtagagcacc ggcacgccgt ccgcctcgga gccgtcgacg atcaggttga cgttgaacct
      361 ctccaggaag cgctccggat cgactcccgg catcatcggc gtctgctgcc ctcgctccag
      421 agcgacgatc ttgtccacgt tcgcaagcag ctcattctgc acccgagcga gatatcgcat
      481 cacctcttcg taaacctggt agcgctggcg gactctggcg agcagcaccg tgatgacctg
      541 ctcggccgtc ttctccacga gctcacgctg ctgccggacg agctcctgct gaaactgggg
      601 ataatcgcga aggcgcgtga tcagtcggtc cgtcattgat tcgatggcct tctggatgcg
      661 ctgacgctcg ccttcgtcga gcgccatgaa ggcatcctct tccatcacct tgccgtcact
      721 cacgggggca aagatgaagc cgttcggcga gggcagcatg gcgagttcca gctcccgcgc
      781 ctcggcttca agggcagcgg cgtcgctgct ctgacgctgc tgcagagagc ttgccagctg
      841 ctggctcttc tgacggtact cgtcggacac cagcgcagcc ggcaccatgg tccgcacgct
      901 gctgatgaac ttctggacgt ggtgccggaa acggcgcccc acaccagccg gcagacgcag
      961 gctgatcggt cgcgccgggt cttcgaagtt ttgcaggtag caccagtcgt cgggagcgtc
     1021 ctggccgccg gcaagctcct tcaaccgcgc aaggatcagc gccctcttgt cggtctgcgc
     1081 cggcccgagg gcgtacacgt tgtagccggg aaacttgatc ccgacgccga agtcgacggc
     1141 ctcccgggcc cgggtctgag ccaccgggcc ctccaacgcc acgacgtccg ccgtggtcgc
     1201 aaaggggaat ccggtttcgt ccacacggcg aagcagctgc tcgggattga gttcgtttgc
     1261 catgccgcct ttctagcctc ccctcccggc acccgctatc gtggattgcc gcagtatcag
     1321 atgcgccgcc ccagctcggc cgccccatga atggcggaca tgatgctgct gcgcgcccgc
     1381 acgtcgccaa tgaggtgagg cgtgtggccc tgggccgtga gcgcctcggc gatttctctg
     1441 ttgggtgcgt tgaaagacac caggatcacc gtgcgcgccg cgatgcgtcg gctgcgctcg
     1501 gtgtaaagca cccccacttc cacctcctgg gggttgatcg agcgcagata gtgcccgccg
     1561 acgaaatcga aatcaccgga catcaatctc tcccgggccg cgccaacggt aacgggcggg
     1621 taagggaggg tctcgccgat ggcgttcagg cgcgaaacca tggtcaccgg cacgccggcg
     1681 ccgagcagca cgtcggccac cgagatcgcc tcaaacgagc cggtgtcgtc gaacaccacc
     1741 gccggcccct cgatgtgggt gatgtcgcca aagccgagca ggtcccagga ggagtagacg
     1801 tggggcaggt cgtagccggg caccgaggct gcgggagtgg acacctgaaa cccgtccgcg
     1861 cggggcgtcg ttccggttgc gatcaccacc tcgtccggag cccgctcggc aatcatgtca
     1921 ggctccacca tggagttgag atgcacggtc acgccgagcc cgtcgatctc ctccgccagc
     1981 cagctcgtga tggcgccgag gtccgcccgg tgaggcgctg aagccgccat ggtcacctgc
     2041 ccgccgagca ccttcatggc ttcgtgaagc tccacctcgt gaccgcgcat ggcagccgtg
     2101 cgcgcgaact cgaggccggc cggcccgccg ccgaccacca tgatgcgttt gggtgccgcg
     2161 gccttggtct cgggatcgtg ggtcactttg gcctcgcggg ccgcggctat gttcaccacg
     2221 cagctgatgc gtccggcggt catgagctgg ccgacgcatc ccatgttcga cccgatgcac
     2281 ggccggatct tgtgctcgtc ccggcggcgc gccttgttaa ccagctccgg atcggcgatc
     2341 agcgcccgga ccatggaaac catgtcggcc gcgcccgtgt cgacgatgtg gctggcatgg
     2401 tcgagggtca tgatgcgccc gaccacgatg gtcggcttcg aaaccaccct cgtgatcggc
     2461 tcgttagagg gcatctccac cccgaggggg tcgtcggagg gcgcgatgag cttgtggaac
     2521 cgccagtagg cccccatgtg gagcgagacg tagtcgacaa gcggatcaac gcgctgggca
     2581 atctccgcgt tcatctccgg cgtcaggccg ccggggacgt agtcgtgatt cggcaggcgc
     2641 acgcccacgg cgaaggccgc gtccccaagc tcgctgcg
//
LOCUS       DBNHOX010000477         4143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_359924, whole genome shotgun sequence.
ACCESSION   DBNHOX010000477 DBNHOX010000000
VERSION     DBNHOX010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_359924"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /gene="glmM"
                     /locus_tag="AC16UZ_10530"
     CDS             complement(<1..427)
                     /gene="glmM"
                     /locus_tag="AC16UZ_10530"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439606.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR6247160.1"
                     /translation="MEKKYFGTDGIRGRVGDAPITPEFCLRLGWAAGRVFARSGKCHV
                     LIGKDTRISGYMLESVLESGLVSAGANVSLLTVMPTPAVAYLTRTLRADAGIVISASH
                     NPYCDNGIKFFDGSGNKLPDAVELEIEAELNREMACVDSD"
     gene            complement(475..1350)
                     /gene="folP"
                     /locus_tag="AC16UZ_10535"
     CDS             complement(475..1350)
                     /gene="folP"
                     /locus_tag="AC16UZ_10535"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR6247161.1"
                     /translation="MAIPPGSTDSPAAVFCRGRKLELEHPRIMGVLNVTPDSFSDGGR
                     LMSRGRVDVERVVAAAHAMIAEGAAILDVGGESTRPGSLPVAVADECSRVIPVIERLS
                     ELDTIVSVDTSKPEVARLALQAGCHLVNDVTGLRDPGMRKVVAEADAAACVMHMRGEP
                     RSMQNEPVYKDVVAEVRAFLEERVGACDEAGIDRQRLLLDPGIGFGKRLEHNLALLRN
                     LEGLRFAGLPVLVGVSRKSMLGALTERPVTDRDAMSAVAAALAVEHGASVVRTHDVGL
                     TADALKLVTAWHGHG"
     gene            complement(1325..3211)
                     /gene="ftsH"
                     /locus_tag="AC16UZ_10540"
     CDS             complement(1325..3211)
                     /gene="ftsH"
                     /locus_tag="AC16UZ_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR6247162.1"
                     /translation="MGKNLLLWLIIAAVLLTVFNNFNVKPEPESLSYSAFIDQVRNDQ
                     VDKVEIEGLIIRGVRDSGEEFQVTRPDLYDPKLLDDLYNHDVQIMGKLPEQQSIWAQL
                     LIASFPILIIIAVFMLFMRQMQGGAGGRGGPMAFGKSKARLLSEDQIKTTFADVAGVD
                     EAKEDVQELVEFLRDPGKFQRLGGRIPRGILMVGQPGTGKTLLAKAIAGEAKVPFFSI
                     SGSDFVEMFVGVGASRVRDMFEQAKKQAPCIIFIDEIDAVGRHRGAGLGGGHDEREQT
                     LNQLLVEMDGFEPNDGIIVIAATNRPDVLDPALLRPGRFDRQVVVSLPDIRGREQILK
                     VHMRKVPLADDVDPAIIARGSPGFSGADLAILVNEAALFAARGGKRLVEMEEFDKAKD
                     KIMMGAERKSMVMSEKEKRNTAYHEAGHAIVGRLMPDHDPVYKVSIIPRGRALGVTMF
                     LPVEDRYSLSRQAVISQICSLFGGRIAEELILGPEFVTTGASNDIERATALARNMVTK
                     WGLSEKLGPLKYDEDDGEVFLGMSAGTKTKPIGDKTKDAIDKEIRDIIDTSYTTAKQL
                     LEDNIGKLHKMAEALVEYETLSSDQIDDIMAGAKPRAPGNPSPPSGKAPSGESPIGDP
                     AGEH"
     gene            complement(3335..3874)
                     /locus_tag="AC16UZ_10545"
     CDS             complement(3335..3874)
                     /locus_tag="AC16UZ_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013856.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR6247163.1"
                     /translation="MSRAHFKLDQLDRRFRLISPGMWVLELGAAPGGWTAYLESRLGP
                     SGRLIVCDSRPVHAGADTVVVSGHFGDAVVDEAIAGALGDRGVDLVLSDMSPNITGIR
                     TRDQAETLDLAELAMAAAERWLKPGGVLVVKMFMGEGTDAWIRQIGKKFAKIRRAKPD
                     ASRQESREIYVVGQQFLAQ"
     gene            4035..>4143
                     /locus_tag="AC16UZ_10550"
     CDS             4035..>4143
                     /locus_tag="AC16UZ_10550"
                     /inference="COORDINATES: protein motif:HMM:NF014087.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HIR6247164.1"
                     /translation="MDAATRKRLKQIAHHLNPVVSVGGRGLSDELVAEIE"
BASE COUNT          768 a         1393 c         1208 g          774 t
ORIGIN      
        1 gatccgagtc cacgcacgcc atttcgcgat tcagttcggc ttctatctct agctcaacgg
       61 catccggcag cttgtttccc gacccgtcga aaaatttgat gccgttgtcg cagtagggat
      121 tgtgcgaagc gctgatgacg atgcccgcgt cggcccgcaa cgtgcgcgtc agatacgcaa
      181 cggctggcgt aggcatgacg gtgagcaggc tgacgttggc cccggcggaa acgagaccgg
      241 actccagaac ggactcgagc atgtaccccg atatgcgcgt gtccttgccg atgagcacgt
      301 ggcacttgcc gctgcgcgcg aacaccctgc ccgccgccca gccaagacgc agacagaact
      361 cgggtgttat gggagcgtcg ccaacccgac cacgaatgcc gtcagtcccg aagtactttt
      421 tttccatccc aagcgcgtcc ttaccaaaac caaagccaga ccaaggcccg gtcttcatcc
      481 gtgcccgtgc caggccgtca ccagcttcag cgcatcggcg gtgaggccga cgtcgtgggt
      541 cctcaccacg ctggcgccgt gctcaacggc gagcgcggct gccaccgcgc tcattgcgtc
      601 ccggtccgtc accggacgct cggtgagcgc accgagcatg ctcttgcgcg aaacgcccac
      661 gagaaccggc aaaccggcga accgcagccc ctcgaggttt ctgagaaggg ccagattgtg
      721 ctcgagacgc ttgccgaatc ctatccctgg atccagaagc aagcgctgtc tgtcgatgcc
      781 tgcttcatcg caagcgccta cgcgctcctc caggaatgcc cgcacttcgg caaccacatc
      841 cttatagacg ggctcgttct gcatggaccg cggttcgcct cgcatgtgca tgacgcaggc
      901 ggcagcatcg gcctcggcca ccaccttgcg catcccggga tcccgcagac cggtcacgtc
      961 gttcacaagg tgacatcccg cctgcagggc caggcgcgca acctccggct tgctcgtatc
     1021 tacggagacg atcgtatcaa gctcgctcag gcgttcgatg acggggatca cacgcgagca
     1081 ctcgtccgct accgccaccg gcagggagcc cgggcgggtc gattcgcccc ccacgtccag
     1141 gatggcggcg ccctcggcga tcatcgcgtg cgccgccgcg accacccgct ccacgtccac
     1201 tcggccacgg ctcatcagac gcccgccgtc ggagaaggaa tcaggggtga cgttgagcac
     1261 ccccatgatt cgggggtgct cgagttcgag ctttcggccg cggcagaata ccgcggccgg
     1321 gctgtcagtg ctccccggcg ggatcgccaa tgggcgattc cccggagggc gctttgcccg
     1381 agggcggaga cgggttcccc ggcgccctag gcttggcgcc ggccatgatg tcgtcgatct
     1441 gatcgctgga gagggtctcg tactccacca gcgcctcggc catcttgtgg agcttgccga
     1501 tgttgtcctc gagcagttgc ttggccgtcg tgtaggaggt gtcgatgata tcccgaatct
     1561 ctttgtcgat cgcgtccttc gtcttgtcgc cgatcggctt ggttttcgta cccgcggaca
     1621 tgccgaggaa cacctcgccg tcgtcctcgt cgtacttcag aggtcccagc ttctccgaca
     1681 ggccccactt ggtgaccatg ttgcgggcca gcgcagtggc acgttcgatg tcgttggacg
     1741 cgccggtggt cacaaattcc ggcccgagaa tgagctcttc ggctatccgc ccaccgaaca
     1801 ggctgcaaat ctggctgatg accgcctgcc gactcagact gtagcggtct tccacgggaa
     1861 gaaacatcgt tacacccagg gcccggccgc gcggaatgat actcaccttg tagaccggat
     1921 cgtggtcggg catgagccga cccacaatgg cgtgcccggc ttcgtgatag gcggtgtttc
     1981 gcttctcctt ctccgacatg accatggatt tgcgctcagc gcccatcatg atcttgtcct
     2041 tggccttgtc gaactcttcc atttcgacca gccgcttgcc ccctcgagcg gcaaacagcg
     2101 cggcttcgtt caccaggatc gccaggtcgg ccccggagaa accaggggag ccgcgggcga
     2161 tgatggccgg gtccacatcg tcagccaggg gtaccttgcg catgtgaacc ttcagaatct
     2221 gctcgcggcc cctgatatcc ggaaggctga ccaccacctg acgatcgaaa cggcccggac
     2281 gcagcaacgc cgggtccagc acgtccggcc ggtttgtcgc cgcgatcacg atgatgccgt
     2341 cgttcggctc gaacccgtcc atctcgacga gcagctggtt gagcgtctgc tcccgctcgt
     2401 cgtgaccgcc acccagacca gcaccccggt gccggccgac cgcatctatc tcgtcgatga
     2461 agatgatgca aggcgcctgt tttttggcct gctcgaacat gtctctgact cgggaagcac
     2521 ccacgcccac gaacatctcg acgaagtcag agcctgaaat cgagaagaac gggaccttcg
     2581 cttcaccggc gatcgccttg gcaagcaggg tcttacccgt tcccggctgg ccaaccatga
     2641 ggataccgcg gggaatgcgc ccaccaaggc gttggaactt gccgggatcc cgcagaaact
     2701 ccaccagctc ctgcacatcc tctttcgctt cgtccacacc ggcgacgtcg gcgaacgtgg
     2761 tcttgatctg atcctctgac agcaatcggg ccttgctctt gccgaaagcc atgggcccgc
     2821 cgcggccgcc ggcaccgccc tgcatctggc gcatgaacag catgaaaacc gcgatgatga
     2881 tgaggatggg gaagctcgcg atgagcagct gcgcccagat gctttgctgc tcgggcagct
     2941 tgcccatgat ctgaacgtcg tgattgtaaa ggtcgtcgag cagcttggga tcgtagaggt
     3001 cggggcgggt cacctggaac tcttccccgc tgtctcggac accgcgaata atcaggccct
     3061 cgatttcgac cttgtccacc tgatcgttgc gcacctgatc gatgaaggcg gagtaactca
     3121 gggactctgg ctccggcttc acgttgaagt tattgaacac cgtcagcagc acggccgcga
     3181 tgatcagcca gagcagcaaa ttttttccca tatctgtcaa gaacttatcc tcaaaactcg
     3241 gcgcagggga ctcgtatcgt gcgtccgtaa ccgcgggcgg caacagcagg caggcagacg
     3301 cgatcggcgc ccatctgaat agcctacacc actcctattg agcaaggaac tgctgcccaa
     3361 caacgtatat ctcgcgggac tcctgccggg acgcgtcggg tttcgctcgg cgaattttag
     3421 caaatttttt gcctatttgg cgaatccagg catccgtgcc ttcgcccatg aacatcttga
     3481 ccaccaaaac gccgccgggt ttcaaccagc gttccgcggc cgccatggcg agttccgcca
     3541 aatccagagt ctcggcctga tcccgcgtgc ggataccagt aatgttgggg gacatgtccg
     3601 aaagtacaag gtccacgccg cgatcaccca aggctcccgc gatggcttcg tccaccaccg
     3661 cgtcaccgaa atgcccgctc accactacag tatccgcccc agcgtgaacg ggtcgtgaat
     3721 cgcacacgat cagcctgccc gacggtccca gacgggactc gaggtaggcc gtccagccgc
     3781 cgggagcggc gccgagctcc aggacccaca tgccaggaga gatcagccgg aatcgtcggt
     3841 cgagctgatc gagcttgaag tgcgcgcggg acacgctccc ctcctgcacc gcacgccggg
     3901 catacggatc gttccgctgg cgctcaagcc aacggccgct ggagcccccc cgcttcttgg
     3961 atccgcgcgt actcatcgtt tcactatgtc acacgttggc cctagaatgc gcccccaccc
     4021 cgggggacac cgacatggac gccgcaacgc gcaagcgcct gaagcagatt gctcatcatt
     4081 tgaatccggt cgtcagcgtt ggcgggcgcg gattgagcga cgagctcgtc gccgagatcg
     4141 agc
//
LOCUS       DBNHOX010000478         3103 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_938245, whole genome shotgun sequence.
ACCESSION   DBNHOX010000478 DBNHOX010000000
VERSION     DBNHOX010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_938245"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2140
                     /gene="leuS"
                     /locus_tag="AC16UZ_10555"
     CDS             <1..2140
                     /gene="leuS"
                     /locus_tag="AC16UZ_10555"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR6247165.1"
                     /translation="RQLKAIGGMYDWDHTVDTTDPAYYKWTQWLFLQLFKAGLAERKE
                     APVNWCPSCMTVLANEQVIQGLCERCDSAVEQRHIAQWFFRITEYAQRLLDNLEHIDW
                     SESTRKAQQNWIGRSEGATLRFPVIEGGNGDPGTVDPLTVTADGDVIEVFTTRPDTLF
                     GATFMVLSPEHPLVASITTDAQQAAVAAYQAQAAKTDLVSRQKADKEKTGVFTGGYCR
                     NPANGAPIPVWIADYVLMEYGTGAIMAVPGGDERDFEFATAMGIEIVRVVAGEGDGAD
                     TPLAEAYTGDGVLVNSGPFDGLGVADGKRAVTAWLGERGLGELKVNYRLRDWCISRQR
                     YWGPPIPIVHCDACGAVAVPEDQLPVELPRVDDFKPDDTGVSPLARVASWYETTCPGC
                     DGPARRETDVSDTFLDSSWYFLRYPSTDRNDAPMDDGITARWLPVDSYIGGNEHAVLH
                     LMYSRFINMVLKDLGHIDFEEPFVKFRAHGLITRNGAKMSKSRGNVITPDAIIDQYGA
                     DTFRTYLMFMGPLEEGGDYRDEGIQGPFGFLHRLWDAVVTAKPDTAADEDVERKLHKT
                     IKQATEQIPELGYNTAIAAMMECLNAVRAGGRQPAVAEVEPLVVMIAPFAPHLAEELW
                     ERLGHGKSIFAGANWPAYDEAKVRDEVVSVAVQVNGKLRGTISLAPGTDQAEAEALAR
                     AEENVARHLDNAEVRRVIYVPNRLLNFVVG"
     gene            complement(2199..>3103)
                     /locus_tag="AC16UZ_10560"
     CDS             complement(2199..>3103)
                     /locus_tag="AC16UZ_10560"
                     /inference="COORDINATES: protein motif:HMM:NF023203.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobaltochelatase CobT-related protein"
                     /protein_id="HIR6247166.1"
                     /translation="EFDETVRAEELCDADEMIRLRALLDQQLVPLQHATSKLANRLQR
                     KLMAKQSRTWEFDLDEGMLDASRLSQVIIDPMNPLAYKQEKEMQFRDTVVTMLIDSSG
                     SMRGRSITIAAMCADILGRTLERCSVRVEVLGFTTRAWRGGQSREKWIASGKPSNPGR
                     LNDLRHIIYKAADDPWQRTRRNLGLMMREELLKENIDGEALIWAHNRLVVRHEERKVL
                     MVISDGLPVDNSTLLVNPSNYLEQHLKYAIDQIENYSSVELIAIGIGHDVTHHYQRAV
                     TITDAEQLGGAMTEQLAELFEAKR"
BASE COUNT          527 a         1040 c         1038 g          498 t
ORIGIN      
        1 ccggcagctg aaggctatcg gcggcatgta cgactgggac cacaccgtgg acaccacgga
       61 ccccgcctac tacaagtgga cccagtggct gttcctccag cttttcaagg cgggccttgc
      121 cgagcggaaa gaggcgccgg tgaactggtg cccctcgtgc atgacggtgc tcgccaacga
      181 gcaggtgatc caggggctgt gcgagcgctg cgacagcgcc gtggagcagc gccacatcgc
      241 ccagtggttc ttccgcatca ccgagtacgc ccagcgcctg ctggacaacc tggagcacat
      301 cgactggtcg gagagcaccc gtaaggctca gcagaactgg atcggccgca gcgagggggc
      361 aaccctgcgt tttccggtga tcgagggggg caacggggac ccgggcaccg tcgaccccct
      421 gaccgtaaca gctgacggcg acgtgatcga ggtcttcacc acacgcccgg acaccctgtt
      481 cggcgccacc ttcatggtgc tctccccgga gcacccgctg gtcgcctcaa tcaccacgga
      541 cgcgcagcag gcggccgtgg ccgcctacca ggcccaggcg gcgaagacgg atctggtgag
      601 ccggcagaaa gcggacaagg aaaagaccgg cgttttcacc ggcggctact gccgcaaccc
      661 ggccaacggg gcgcccattc ccgtgtggat cgccgactac gtgctcatgg agtacggcac
      721 cggcgccata atggcggtgc cgggcgggga cgagcgggat ttcgagtttg caaccgccat
      781 gggcatcgag atagtccgcg tggtggccgg ggaaggcgac ggggccgaca cgccgctggc
      841 cgaggcatac accggcgacg gcgtgctggt gaattccggc cccttcgacg ggctcggcgt
      901 ggctgacggc aagcgcgcgg tcaccgcgtg gctcggcgag cggggcctcg gcgagctcaa
      961 ggtcaactat cggctccggg actggtgcat ctcccgacag cgctactggg gaccgcccat
     1021 ccccatcgtg cactgcgatg cctgcggcgc ggtggcggtg cctgaggatc agctgccggt
     1081 ggagctgccc agagtcgacg acttcaagcc cgacgacacc ggcgtgagcc cgctcgcccg
     1141 ggtggccagc tggtacgaaa ccacctgccc cggctgcgat ggcccggcgc gccgcgaaac
     1201 cgacgtgtcc gacaccttcc tggactcctc ctggtacttc ctgcgctacc cgtccacgga
     1261 ccggaacgac gcgcccatgg acgacgggat caccgcccgc tggctgccgg tggacagcta
     1321 catcggcggc aacgagcacg ccgtgctgca cctgatgtac tcgcgtttca tcaacatggt
     1381 gctcaaggac ctcggccaca tcgatttcga ggaacccttc gtgaagttcc gcgcccacgg
     1441 gctcatcacc cgcaacggcg cgaagatgtc caaatcccgg ggcaacgtca tcaccccgga
     1501 cgccatcatc gaccagtacg gcgcggacac ctttcgcacc tacctcatgt tcatgggccc
     1561 cctggaggaa ggcggcgact accgggacga gggcatccag ggccccttcg ggttcctgca
     1621 ccggctgtgg gatgcggttg tgaccgccaa gcccgacacc gctgcggacg aggacgtgga
     1681 gcgcaagctg cacaagacca tcaagcaggc caccgagcag attcccgagc tcggttacaa
     1741 cacggccatc gccgccatga tggagtgcct gaacgccgtg cgcgccggcg gcaggcaacc
     1801 ggcagtggcg gaggtggagc cgctggtggt gatgatcgcg ccctttgccc cccacctggc
     1861 cgaggagctg tgggagcgcc tcggacacgg caagagcatc ttcgcgggcg caaactggcc
     1921 ggcctacgac gaggccaagg tccgcgacga ggtggtctcg gtggccgtgc aggtcaacgg
     1981 caagctgcgc ggcaccatca gccttgcacc cggcaccgac caggctgaag ccgaggcgct
     2041 ggcccgggcc gaggagaacg tggcccgcca tctggacaac gccgaagtgc gtcgcgtcat
     2101 ctacgtgccc aaccgcctgc tgaacttcgt ggtcggttag tcgtcgtgtc ggggaccgca
     2161 ggctcccgtt tgaagtcggc ttcccagggc ccggcccgtc agcgcttggc ctcgaagagc
     2221 tccgccagct gctcggtcat ggcaccgccg agctgctccg catccgtgat ggtgaccgcg
     2281 cgctggtaat ggtgagtcac gtcatgcccg atgccgatgg cgatgagctc caccgacgag
     2341 tagttctcga tctggtcgat ggcgtacttc aggtgctgct ctaggtagtt gctcgggttc
     2401 accagcagcg tggagttgtc caccggcagg ccgtcggaga tgaccatgag caccttgcgc
     2461 tcctcgtgac gcaccaccag gcgattgtgg gcccagatga gcgcctcgcc gtcgatgttc
     2521 tccttcaaca gctcctcgcg catcatgagg ccgagattgc ggcgcgtgcg ctgccagggg
     2581 tcgtcggccg ccttgtagat gatgtggcgg agatcgttca ggcgccccgg gttcgacggc
     2641 ttgcccgagg cgatccactt ctcccgggac tggccgcccc gccaggctcg cgtggtgaaa
     2701 ccgagcacct ccacccgcac ggagcagcgc tcgagggtgc ggcccagaat gtccgcgcac
     2761 atggcggcga tggtgatgga ccgtccgcgc atggatccgg agctgtcgat gagcatcgtg
     2821 acaaccgtgt cgcgaaactg catctccttt tcctgcttgt aggccagcgg gttcatggga
     2881 tcgatgatca cctgggatag ccgcgaggcg tccagcatcc cctcgtccag atcgaactcc
     2941 caggtccggc tctgcttggc catgagcttg cgctgcagcc ggttggcgag cttggaggtg
     3001 gcatgctgca gggggacgag ctgctggtcg agcagcgcgc ggagccggat catctcgtcg
     3061 gcgtcgcaca gctcctcggc gcggacggtc tcgtcgaact cct
//
LOCUS       DBNHOX010000479         1783 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_938375, whole genome shotgun sequence.
ACCESSION   DBNHOX010000479 DBNHOX010000000
VERSION     DBNHOX010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_938375"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="AC16UZ_10565"
     CDS             <1..424
                     /locus_tag="AC16UZ_10565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6247167.1"
                     /translation="LNLIYQFWVHTQAVDRLPAWVELIFNTPSHHRVHHASNVRYLDC
                     NHAGVLIVWDRLFGTFSEETAEDPPVYGLTRNVRSFNPAVVLFHEYAALWRDVRRAPG
                     WRQKLGYLLRAPGWSHDGEDRRSRTLRRCEAATAGTGP"
     gene            complement(402..1592)
                     /locus_tag="AC16UZ_10570"
     CDS             complement(402..1592)
                     /locus_tag="AC16UZ_10570"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6247168.1"
                     /translation="MRHALGAPRQGQEQTNMSYADGRLINDADSHLMELPDFLTAHAD
                     PGMRGRLPELNPGGQFDMSERTHQRGHPPEVVAELTALGNGLTRGPKWHDALGASSGH
                     ERRTALDLLGFQRQVVFSSFCARLIFEAEPLDIRFAAARAHNRAMAEFCSADERLIGV
                     AMVPLDDPNAAVREIAHAHELGLGAVWIAADAPGGRSPGHPEHDPIWATLSERRLPFI
                     LHVGSGVLGIPDSWMNDGRPESESARGGAEIIGSKDLTVIHHSAVRFLSVMVLDGVLE
                     RHPELRGGVIEIGAGWVPDMLRRLDHAAAIWSRSEPHLATMSRSPSRQIRDQVRFTPY
                     PFEDVAQLVRESYPELYLFSSDYPHAEGGRDPIGRFDAATATLTDSERAQFFSRNFTD
                     LYRQ"
     gene            1650..>1783
                     /locus_tag="AC16UZ_10575"
     CDS             1650..>1783
                     /locus_tag="AC16UZ_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016486814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6247169.1"
                     /translation="MSDAFRLDGQVAVVTGAGKGIGRGIALCLAQAGADVVVSARTLS
                     "
BASE COUNT          304 a          543 c          627 g          309 t
ORIGIN      
        1 gctcaacctg atctatcagt tctgggtgca cacgcaagcc gtggatcggc ttcccgcctg
       61 ggtggagctc atcttcaaca cgccttcgca tcaccgggtt catcacgcca gcaacgtccg
      121 ctacctggac tgcaaccatg cgggtgtgct gatcgtctgg gatcgactgt tcgggacgtt
      181 cagcgaggag acggcggagg atccgcccgt gtacgggctg acgcggaacg ttcgctcttt
      241 caatcctgcc gtggtcctgt tccacgagta cgcggccttg tggcgtgacg tccggcgcgc
      301 gcctggatgg cgccagaagc tcggctatct tctccgggct cccggctgga gccacgacgg
      361 cgaggaccgg cgatcccgga cgctgcggcg ttgcgaggcc gctactgccg gtacaggtcc
      421 gtgaagttgc gcgagaagaa ctgggctcgc tcggaatcgg tcagcgtggc ggtggccgcg
      481 tcgaagcggc cgatggggtc gcgtccgccc tcggcatgag ggtaatccga cgagaagaga
      541 tacagctcgg gataggactc acggacgagt tgggcgacgt cctcgaaagg atagggcgta
      601 aaccgcacct gatcgcgaat ctgccgggac ggcgatcggg acatggtggc caggtgcggc
      661 tcggatcggg accagatggc ggcggcgtgg tccagccttc tcagcatgtc gggcacccag
      721 cctgcgccta tctcgatcac gccaccgcgc aattccgggt gccgttccag gacgccgtcg
      781 agaaccatga cggacagaaa acggacggcg ctgtgatgga tcacggtgag gtcctttgac
      841 ccgatgatct cggcgccgcc gcgggccgac tcgctttccg gccggccatc gttcatccac
      901 gaatcgggga tgccgagcac gccgctgccc acgtgcagta tgaaaggcag acggcgctcg
      961 ctgagggtgg cccagatggg atcgtgctcg gggtgcccgg gagaacgtcc tccgggcgca
     1021 tcggcggcga tccacacggc gcccaggccg agctcgtgag cgtgggcaat ctcgcgcacg
     1081 gcggcgttgg ggtcgtcaag cggcaccatg gccacgccga tcagccgctc gtctgcactg
     1141 cagaattcag ccatcgctcg gttgtgcgcc cgggccgcgg cgaagcgtat gtccagaggt
     1201 tccgcctcga agatcagccg cgcgcagaac gaagagaaca cgacctgacg ctgaaaaccg
     1261 agcaggtcca gggcggtacg acgttcgtgc ccgctggaag cgccaagcgc gtcatgccat
     1321 ttcggcccgc gcgtcaggcc gttgccgagc gccgtaagct cggcgacgac ctcgggtggg
     1381 tgaccgcgct ggtgggttcg ctcgctcatg tcgaactggc ctcccggatt gagctcggga
     1441 agacggccac gcatgcccgg gtccgcatgg gcagtgagga agtccggcag ctccatcagg
     1501 tggctgtcgg catcgttgat cagacgccca tcggcgtagc tcatgttggt ctgctcctga
     1561 ccctggcgcg gcgcgcctag tgcgtgccgc atgaatcatg ccgggaaggg tttagtatag
     1621 tacgaagctt tcaaagagag ggtgtgacga tgtccgacgc gtttcgactg gacggccagg
     1681 tggcggtggt tacgggggct ggtaagggaa tcggccgggg aatagcgctt tgccttgcac
     1741 aagcaggggc agacgtggtg gtttcggccc gcacgctgag cga
//
LOCUS       DBNHOX010000480         3618 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1709800, whole genome shotgun sequence.
ACCESSION   DBNHOX010000480 DBNHOX010000000
VERSION     DBNHOX010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1709800"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="AC16UZ_10580"
     CDS             complement(<1..570)
                     /locus_tag="AC16UZ_10580"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017129865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6247170.1"
                     /translation="MTQDTDQSRRAARLLEVLDLECIEQNLFLGANEVRGEFRLFGGQ
                     VLSQSLRAACLTVEGRSAHSLHGYFMRAGNAGRPVLYEVDRIRDGRSFATRRVVAIQS
                     GEAIFSLDVSFQVDEAGFEHAHPMPNVPPPEELEGDIAVVSRLTGDDPHLSPMAGRPR
                     PFELRSVFKPGSDEWAANRHWNPVWIRFAG"
     gene            991..2820
                     /locus_tag="AC16UZ_10585"
     CDS             991..2820
                     /locus_tag="AC16UZ_10585"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6247171.1"
                     /translation="MAARQSINLWQLTRGQRLRYAGAIFAMGMTNLFIFGPVLVSGSA
                     IDVIADQDLSHAVPPLLWAASRFSEGAPFAAYLWVAAVVAVTMTAIGGAFLFARGRFA
                     AVASEAIVRNLRERLYDRLHDLEAGFFDTADTGDLVQRASSDVETLRVFLASDVVEIG
                     RAIMLIMCVAPILAWLDVRLAALSLCLMPLLAIGAYLFFARVKDVFQITDESEAAMTA
                     TLQENLTGIRVVRAFARQAFEIEKFGARNAEFRNNNRRLIQLMGIYWSTSDLFAMTQI
                     GIVLIAGGLFVLDGSITVGTLFEFMTMVSMVIWPVRQLGRVLTDTGKAVVSLGRINEV
                     LTEAEERAGTIPGEGRARGEIRIENLTFGYDPQRPVLEDVNLHVEVGETLAIVGPPGS
                     GKSSLIRVLLRMYPYQKGSVRIDGWEVAELDRLWLRHQIGVVLQDPFLYSRSIRANLA
                     LGRPGAPEKELIEACHDAAVHDAIIGFPEGFEAMVGERGVTLSGGQRQRMALARALLK
                     DPPILVLDDSLSAVDTGTERRILDAMRRRKGRQTTLIIAHRLSSVMHADRILVLQEGR
                     VVQLGNHATLAGEPGPYRTLCEIQGALDASIEADTNATGRGNG"
     gene            2813..>3618
                     /locus_tag="AC16UZ_10590"
     CDS             2813..>3618
                     /locus_tag="AC16UZ_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR6247172.1"
                     /translation="MDNYDDENFDDGFDDEALAARVDLKLWRKLFAYARRYPKDLVWL
                     AVCAFTTASMEVTYPLITKWVVDDVDAAYAAGTVISLWPHALAYLACTVTICIAIGGF
                     IWMGGKIRTHVSHDIRRDGFENLQRLSFAFYDYRPVGWLMARMTSDCERLSNILAWGF
                     LDLVWGSTMMLGIAVAMLVMNPKLALVVLAILPLLGWVSARFQRRILSSARRVRATNS
                     RITASFNEAIMGVLTSKAFVREQANLDEFRELTNTMHGASVLNLSQAAVYV"
BASE COUNT          612 a         1216 c         1158 g          632 t
ORIGIN      
        1 gcccgcgaag cggatccaca cggggttcca atgccggttt gccgcccact cgtcgctgcc
       61 cggcttgaac accgagcgaa gctcgaacgg ccgcggccgg ccggccatgg ggctcaagtg
      121 cggatcgtct cccgtcagcc ggctcaccac ggcgatgtcc ccctccagct cctcaggggg
      181 cggcacgttg ggcatggggt gcgcatgctc gaatcccgcc tcgtcgacct ggaaggagac
      241 gtccaggctg aagatcgcct cccccgattg gatggccacc acgcgtcggg tagcaaagct
      301 cctgccgtcg cgaatgcggt ccacctcgta gagcacgggt ctccccgcat tgcccgcgcg
      361 catgaagtaa ccgtgaagag agtgagccga gcggccctcg acggtcaggc aggcggcccg
      421 cagggactgc gacagaacct gtccgccgaa gaggcggaac tcgccgcgga cctcgttggc
      481 acccagaaag agattctgct cgatgcattc caggtccagc acttccagca ggcgcgccgc
      541 gcgtcgcgat tgatccgtgt cctgagtcat ggcgtgcatt gtagattcac gagggaatga
      601 cgaataccca cggccggtaa ccggatcctg cgggcgccgg ccgcgcgccc ggcggcggct
      661 cccgacgcgg tctcgtgggg cccaggcgtt gaacctgccc acggatgcca gtaagcttgc
      721 cgccctgttt gactcaccgt cccgtcagga ggccaccatg agctggatga acagccgacc
      781 gaccgttgcc cgatacggag acaacgcgcg aaagcggtga gcccgtcctt cgtctgaaat
      841 agacccccgc cggcatctgc cggcctgtga tgaggacccg ctcaccagcg agcggatccc
      901 cctgcagtca tcttcgaccc cgatccgctc cgcgcctgcc caaccgggcc caggacccat
      961 gcaaccgaga atctgcggga gcgattgacg atggcagcca gacaatcgat caacctatgg
     1021 cagctgacca gaggtcagcg gctccgctat gccggggcca tcttcgccat ggggatgacc
     1081 aacctgttca tattcggccc cgtgctggtg agcggctcgg cgatcgacgt catcgccgat
     1141 caggacctca gccacgccgt cccgcctctg ctctgggcgg ccagcaggtt ttccgagggc
     1201 gcgcccttcg ccgcctacct ctgggtggcc gccgtggtcg cggtcaccat gacggccatc
     1261 ggcggcgcct tcctgttcgc gcgcggccgg tttgcagcgg tggcgtcgga agccatcgtc
     1321 cgcaaccttc gcgagcggct gtacgaccgg ctgcacgatc tggaagccgg ctttttcgac
     1381 acggcagaca cgggcgatct cgttcagcgc gcgagctcgg acgtggaaac gctgcgcgtc
     1441 ttcctggcaa gcgacgtggt cgagatcggt cgggccatca tgctcatcat gtgcgtcgcc
     1501 cccatcctcg cgtggctgga cgtccggctt gccgcgctgt ctctgtgcct gatgcccctg
     1561 ctggccatcg gagcctacct cttcttcgcc cgggtgaaag acgtgttcca gataacggac
     1621 gagtccgaag ccgccatgac cgccacgctc caggagaacc tcaccggcat ccgcgtggta
     1681 cgggcgttcg cgcggcaggc gttcgagatc gagaaattcg gcgcgcggaa cgcagagttc
     1741 aggaacaaca atcgccggct gatccagctg atgggcatct actggagcac gagcgatctg
     1801 ttcgccatga cccagatcgg catcgtgctc attgcggggg ggctgttcgt gctcgacggt
     1861 tccatcaccg tcggcaccct tttcgagttc atgaccatgg tctccatggt gatctggccc
     1921 gtccgccagc tcggccgggt gcttacggat acgggcaagg cggtggtgtc gctgggcagg
     1981 atcaatgagg tgctcacgga agccgaggag cgggccggca ccatcccggg cgaggggcgg
     2041 gcacgcgggg agattcgcat cgagaacctg acgttcggct acgacccgca gcgtccggtg
     2101 ctcgaggatg tgaacctgca cgtggaggtc ggcgagacgc tggccatcgt cggccccccg
     2161 ggatccggca agtccagcct gatccgcgtg cttctgcgca tgtatccgta ccagaaggga
     2221 tccgtgcgga tcgatgggtg ggaggtcgcc gagctcgacc ggctctggct gcgccaccag
     2281 atcggagtgg tcctgcagga cccctttctc tactcgcgca gcattcgcgc caaccttgcg
     2341 ctggggcggc ccggcgcgcc cgagaaagag ctcatcgagg catgccacga cgcggccgtt
     2401 cacgacgcca tcatcggctt cccagaaggt ttcgaagcca tggtcggcga gcgcggcgtc
     2461 acgctttccg gcgggcagcg gcagcgcatg gccctggcac gcgccctgct gaaggacccg
     2521 cccatactgg ttctcgacga ttccctttcc gcagtggata cgggaactga gcgcaggatc
     2581 ctggatgcca tgcgccgccg caagggccgc cagacgaccc tgatcattgc ccaccgactg
     2641 tcgtcggtga tgcacgcgga ccgcattctc gttcttcagg aaggacgtgt ggttcagctc
     2701 ggcaatcacg ccacgctggc cggggagccc ggaccgtacc ggacgctctg cgagatccag
     2761 ggtgcgctgg atgcatccat cgaggcggac accaacgcca caggacgcgg aaatggataa
     2821 ctacgacgac gagaacttcg acgacggctt cgacgacgaa gcgctggcgg ccagggtcga
     2881 cctgaagctg tggcgcaagc tgttcgccta cgcccgacgc tacccgaagg acctcgtgtg
     2941 gctggccgtc tgcgctttca ccaccgcctc gatggaggtc acgtatcccc tgattaccaa
     3001 gtgggtggta gacgacgtcg acgccgctta cgcagccggc acggtgatct ccctgtggcc
     3061 tcatgcgctc gcctacctcg cgtgcacggt gaccatctgc atcgccatcg gcgggttcat
     3121 ctggatgggg ggaaaaatcc gcacccacgt gagccacgac atacgtcgcg acggcttcga
     3181 gaacctgcag cggctgtcct tcgccttcta cgactaccgt ccggttggct ggctcatggc
     3241 gcgcatgacg tcggactgcg aacggctctc caacatcctg gcctggggat tcctcgacct
     3301 ggtctgggga tcaaccatga tgctgggcat cgccgtggcc atgcttgtca tgaacccgaa
     3361 gctggccctc gtggtgctcg ctatcctgcc gcttctcggc tgggtctcgg cccgttttca
     3421 gcgccggatt ctgtccagcg cccgccgcgt acgcgcgacg aattcacgca tcacggcttc
     3481 cttcaacgaa gccatcatgg gcgtgctgac cagcaaggcg ttcgtgcgcg agcaggcgaa
     3541 tctggacgag ttccgggagc tcaccaatac catgcacggc gcatcggtgc tgaatctcag
     3601 ccaggcggcc gtctacgt
//
LOCUS       DBNHOX010000481         3744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_873252, whole genome shotgun sequence.
ACCESSION   DBNHOX010000481 DBNHOX010000000
VERSION     DBNHOX010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_873252"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="AC16UZ_10595"
     CDS             <1..416
                     /locus_tag="AC16UZ_10595"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIR6247173.1"
                     /translation="GQADEPPLALADGTTMKVDDWHGRWLLINYWAEWCAPCRKEIPE
                     LNRLQAEVGEERLLVIGVNFDGLTGQRLTELIEKMDVRFPVLVEDPRGRWDVEQPTVL
                     PSTLVIAPNGRLKEVLVGPHTFESFSRALGLTAST"
     gene            422..910
                     /locus_tag="AC16UZ_10600"
     CDS             422..910
                     /locus_tag="AC16UZ_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247174.1"
                     /translation="MRKLAIRDVLIVAVATAGWLGLLPLSEGRPLISDSLALLLGLGV
                     TVSAYLLHEWGHALAGLVVGSRIHAPASLRSVSLFSFDAEANSKTQFAAMSIGGFVVT
                     AAAMGFVYFVLPDGYMASRVARGGVTVLASITVFIEVPLLLYGLLARRLPGVVAVFRP
                     ET"
     gene            complement(957..1604)
                     /locus_tag="AC16UZ_10605"
     CDS             complement(957..1604)
                     /locus_tag="AC16UZ_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR6247175.1"
                     /translation="MGRFGLSVALLLCAICPLPTPSALADVRIDPVLLEKLAVSMADR
                     YEHTDRFDAEVWLVTSERRLARYMDDHDERLALLQTVYREADRNALDADLVLAVMQVE
                     SAFDRYAISRVGAQGLMQVMPFWRNEIGRPRDNLTDMETNIRYGTTILAHYIEVSDGD
                     LVDALARYNGSRGRLKYPEKVVGAWRRVWRHKSSDELPDLVSTCATYALHACRYR"
     gene            complement(1607..3307)
                     /locus_tag="AC16UZ_10610"
     CDS             complement(1607..3307)
                     /locus_tag="AC16UZ_10610"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR6247176.1"
                     /translation="MRQSMLLIPTMKENPADADVASHRLMLRAGLIRQVASGLYTWLP
                     LGLRVIRRIESIIREEMNQSGAQEVLMPVVQPAELWEESGRWQKMGPEMLRMRDRHER
                     DFCLAPTHEEVITDLFRREVHSYRQMPCNFYQIQTKFRDEIRPRFGVMRAREFTMKDG
                     YSFHLDQACFDDTYRTMYDCYSRILTRMELEFRAVEADTGNIGGANSHEFHVLAESGE
                     DTIAYATHGPYAANLDKARAAPPAAGRSPGDELRKVATPGVETIEAVARFLEVSPAQT
                     LKTLLVRGSSEPVVALILRGDHDLNELKAEKLDEVADPLSFATEAEIQAATGCRPGSL
                     GPIGLDVPAYVDVEAAALTNFVCGANEDGFHYTGANWDRDQTLDTARVVDFRNVVAGD
                     PAPDGQGTLQFLRGIEVGHIFQLGSVYSEPLGAAVLGPDGASITPIMGCYGMGVTRLV
                     AAIIEQNHDEVGIIWPDPVAPYDLHIIALNYGKSETVREAADRLYEACLSDGMEVLLD
                     DRDERPGVKFAEADLIGIPRRITVGERGLKDQTVEYRRRRDSENEVVTLDGMLDRLRS
                     "
     gene            complement(3439..>3744)
                     /locus_tag="AC16UZ_10615"
     CDS             complement(3439..>3744)
                     /locus_tag="AC16UZ_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR6247177.1"
                     /translation="KAKMSKTAMLSEIAEKTNLTRGQVGSVLDELEVLIERHVKKRSV
                     GEFTLPGLLKIRSVKRPASKARMGRNPATGEEIMIKAKPASIRVRVTPLKKLKDMVG"
BASE COUNT          613 a         1190 c         1216 g          725 t
ORIGIN      
        1 tcggccaggc agacgagccg cctctggctc tggccgacgg tacgaccatg aaggtcgacg
       61 actggcacgg gcgctggctg ctgatcaact actgggcgga gtggtgcgcg ccgtgccgca
      121 aggagattcc ggagctcaat cgcctgcagg cggaggtggg cgaggagcgg ctactggtga
      181 tcggcgtcaa cttcgacggc ctcaccgggc agcggctcac ggagctcatc gaaaagatgg
      241 acgtccggtt tccggtgctt gtagaggacc ctcggggcag atgggacgtg gagcagccca
      301 ccgtgctccc gagcacgctg gtcatcgcgc ccaacggtcg cctcaaggag gtgctggtag
      361 ggccgcatac cttcgagagc ttcagccggg ccctgggtct gaccgcgtcc acgtgacgat
      421 gttgcgcaag ctcgccatcc gggacgtgct gatcgtggcg gtggcgaccg ccggctggct
      481 cggcctgctg ccattgtccg aaggacgccc gctcatcagc gattcgctgg ccctgttgct
      541 cggtctcggc gtgacggtga gtgcctacct cctgcacgaa tggggtcacg cgctcgccgg
      601 tctcgtggtc ggcagccgca tccacgcgcc ggcctcgctg cgcagcgtgt cgctgttcag
      661 cttcgacgcc gaagccaaca gcaaaaccca gttcgccgcg atgtccatcg gtgggttcgt
      721 ggtcacggcg gccgcgatgg gctttgtcta ttttgtgctg cccgacggct atatggcttc
      781 ccgtgttgct cggggcgggg tgaccgtgct ggcctcgatc accgttttca tcgaggtgcc
      841 cctgcttttg tacggattgc tcgccaggcg cctgccaggc gtcgtggcgg tattccgccc
      901 ggaaacctga ccgctggacc gttgtttgcc ggaaccgtcg agatcgagac gatgccctat
      961 cggtagcggc aggcgtgcag ggcgtaggtt gcgcaggtgg atacgaggtc gggcagctcg
     1021 tcggacgatt tgtggcgcca gacgcggcgc caggcgccga cgaccttttc cggatacttc
     1081 aggcggccgc ggctgccgtt gtagcgtgcc agagcgtcta ccaggtcgcc gtccgatacc
     1141 tcgatgtagt gcgccagaat ggtcgtgccg tagcggatgt tggtttccat gtcggtgagg
     1201 ttgtcacggg gtcggccgat ctcgtttcgc cagaatggca ttacctgcat gagcccctga
     1261 gcaccgactc gcgagatcgc gtagcggtcg aaagcgctct cgacctgcat gacggcaagc
     1321 accagatccg catccagcgc gtttctgtcg gcttcgcggt agacggtctg aagcagtgcc
     1381 agccgctcgt cgtgatcgtc catgtatctg gcgaggcgtc gctcggatgt gaccagccag
     1441 acttccgcgt cgaaacggtc cgtgtgctcg tagcggtcgg ccatggaaac ggcgagcttt
     1501 tccagcaaga cggggtcgat gcgtacatct gccagcgcag atggcgtcgg cagcgggcag
     1561 atggcgcaca ggagcaacgc cacagacaag ccgaagcggc ccaagatcag ctcctcagcc
     1621 ggtccagcat cccgtcaagc gtcaccacct cgttctccga gtcgcggcgg cgacgatact
     1681 cgacggtctg gtccttcagt ccgcgttcgc ccacggtgat gcgccgtggg attccgatga
     1741 gatccgcctc ggcgaatttc acgcccggcc tctcgtcccg atcatcgagg agtacctcca
     1801 tgccgtcact caagcaggct tcgtagagcc tgtcggccgc ttcgcgaacc gtctccgact
     1861 tgccgtaatt gagcgcaatg atgtgaaggt cgtagggtgc gacgggatcg ggccagataa
     1921 tgccgacctc gtcatggttt tgctcgatga tggccgcgac caggcgcgtc acgcccattc
     1981 cgtagcagcc catgatgggt gtgatgctcg cgccgtccgg tcccagcacc gcggcgccca
     2041 gcggctcgct gtagacgctg ccgagctgga agatgtggcc gacctcgata ccgcgcagga
     2101 actgcagagt gccctggccg tcgggcgccg ggtcgccggc caccacgttc cggaagtcca
     2161 ccacgcgggc tgtatccaga gtctggtcgc gatcccagtt cgccccggtg tagtggaagc
     2221 cgtcttcgtt ggcgccgcaa acgaagttgg tcagggcagc cgcctccaca tccacatagg
     2281 ccggcacgtc gagcccgatc ggtccgaggg atccgggcct gcaccccgtg gctgcctgta
     2341 tctcagcctc cgtggcgaag ctgagcgggt cagccacctc gtccagcttc tccgccttga
     2401 gctcgttcag atcgtggtct ccgcgcagga tcagcgcgac cacgggttcc gaagaaccac
     2461 ggaccagcag ggtcttcagg gtctgggccg gcgagacctc cagaaaacgg gcgaccgcct
     2521 cgatggtctc gacgcccggg gtggcaacct tccggagctc atcacccggc gaacggccgg
     2581 ctgccggcgg cgcggcgcgc gccttgtcca ggttggccgc gtacggtccg tgggtggcgt
     2641 aggcgatggt gtcctcgccc gactccgcga ggacgtgaaa ctcatgggaa ttcgcgccgc
     2701 cgatgttgcc ggtatctgct tccacggcgc ggaactccaa ctccattcgc gtcaggatac
     2761 gggagtaaca gtcgtacatc gttcgatacg tatcgtcgaa gcaggcctgg tccagatgga
     2821 acgagtagcc gtccttcatg gtgaactccc gcgctctcat cacgccgaat ctggggcgga
     2881 tctcgtcacg gaacttggtc tgtatctggt agaagttgca cggcatctgc cggtagctgt
     2941 gtacctcgcg ccggaacaga tcggtgatca cttcctcgtg agtgggcgcc aggcagaagt
     3001 cgcgctcgtg acgatcccgc atgcgcagca tctcgggtcc catcttctgc cagcgtccgg
     3061 attcctccca cagctctgcc ggttgcacca ccggcatcag cacctcctgg gcgccacttt
     3121 gattcatctc ctcacgaatg atcgactcga ttcgacggat cacccgcagc cccaggggca
     3181 accaggtgta aagaccggag gcgacctgtc ggatgaggcc ggcccgaagc atgaggcgat
     3241 gactggccac gtcggcgtcg gccggatttt ccttcatggt gggaatgagc agcatggatt
     3301 gccgcatgtg cagtgccctc tggatcgatt gcaggacgtt tggcctagat tagcgaaatt
     3361 gcagcaaaaa aggggccgaa ggcccctttt ctgccgatga ccgcccgcat cgccagcgtt
     3421 cggtcacgga gccacgatct agccgaccat gtccttgagc ttcttcagcg gggtcacccg
     3481 aacacggatg ctcgctggct tggccttgat catgatctcc tcgccggtgg ccgggttgcg
     3541 gcccattcgc gctttgctcg cggggcgctt gacggaacgg atcttcagca ggcccggcag
     3601 agtgaactca ccgaccgaac gcttcttgac gtggcgctcg atgaggactt cgagctcatc
     3661 gagaaccgag cccacctggc cgcgggtcag gttggtcttc tctgcgatct ccgagagcat
     3721 tgccgtcttg gacatcttgg cctt
//
LOCUS       DBNHOX010000482         2483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_167817, whole genome shotgun sequence.
ACCESSION   DBNHOX010000482 DBNHOX010000000
VERSION     DBNHOX010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_167817"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="AC16UZ_10620"
     CDS             complement(<1..1081)
                     /locus_tag="AC16UZ_10620"
                     /inference="COORDINATES: protein motif:HMM:NF051540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR6247178.1"
                     /translation="MTLQHEDIRREVEQRRFEVNANLLRIYNYYRVFVGLALLAVFMT
                     SLMGSRLGGLDPTAFLWSILAYIAINLTAAVAVQFLPRSIVQGQTISAALVVFDILTL
                     TWLTYLSGGVASGLGLLILVSVSTGSIMVTSRATAVLAATASIAVLFEEFYLSLTAPA
                     LHDDYFQAGVLGVFYFAFSLSIHHLARRIREDDLRAMTQAAELADLERLNRQIVQSMR
                     TGIIVVDADNRVRMMNQSATALLGLAGDEALARLPEPLEERLEDWRENSRLRTPPFQI
                     EPDTPEVRANFSAVRAGGDVTIFLEDTSELQQQAQQLKLAGLGRLSANIAHEIRNPLG
                     AISHAAQLLRESQNLDEGETRLTQII"
     gene            complement(1176..2174)
                     /locus_tag="AC16UZ_10625"
     CDS             complement(1176..2174)
                     /locus_tag="AC16UZ_10625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR6247179.1"
                     /translation="MRKFTLMPFLLPLLLLCLASSGCSWMPWVGKDKDKAEEDLNATE
                     QQLYRDTQRALRAGNYDRAIQGLQQLEARFPFGRYAEQAQLEIIYAQYMSYDHDTARA
                     SADRFMRLHPQHANIDYAYYIKALAAFNKNRGLLDRIFATDLAKRDMTAARDAYADFA
                     QLLALYPDSQYAPDANLRMIYLRNLLAESEIHVADYYMRRGAYLAASNRARYVIETYS
                     KSQSVDDALAILVEANWRLGLEEAANDALRVLALNYPNYPAFDTDGNLTLASTVRNRD
                     RSWTNMVTFGLLDRPKVPPPITIKHPEGFEPPPVNPDAAPPPKKKGGIFSWLPFVG"
     gene            2262..>2483
                     /locus_tag="AC16UZ_10630"
     CDS             2262..>2483
                     /locus_tag="AC16UZ_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013633.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HIR6247180.1"
                     /translation="MAEPLEIQKRVTVPPDVAGERVDKAAARLFPEFSRAALTAWIQD
                     GALTVDGARVKPKVKLVGGETLVLDAVVAP"
BASE COUNT          462 a          695 c          859 g          467 t
ORIGIN      
        1 ggatgatctg ggtgaggcgg gtctcccctt cgtccaggtt ctgcgattcg cgcagcaact
       61 gggccgcatg gctgatggcg cctaggggat tgcggatctc gtgggcgatg tttgcggaga
      121 ggcgacccag accggcgagc ttgagctgtt gagcctgttg ctgaagctca ctggtgtcct
      181 cgagaaatat ggtgacgtcc ccgccggccc gcacagcaga gaagttggca cgcacttccg
      241 gggtatcggg ctcgatctgg aagggaggcg tccgaaggcg cgaattctct cgccagtcct
      301 cgagccgttc ctccagcggc tcgggaagac gcgccagcgc ctcgtcgccc gccaggccca
      361 gtagcgctgt tgcggactgg ttcatcatcc gcacacgatt gtctgcatcg accacgatga
      421 ttcccgtgcg catgctctga acgatctgcc ggttcaggcg ttccaggtct gcaagctccg
      481 ctgcctgggt catggctcga agatcgtcct ctcgaatgcg tctggcgaga tgatgaatgg
      541 aaagggaaaa ggcgaagtag aagacgccca ggacgccggc ctgaaagtag tcgtcgtgaa
      601 gggcgggtgc cgtaagcgag aggtagaact cctcaaatag cacggcaatg ctcgccgtgg
      661 cggccagcac cgcggtggcg cggctggtga ccatgatgga gcccgtcgag acagaaacga
      721 gaatcagcag gccgaggccg ctggcaacgc cgccgctcaa gtaagtgagc caggtgagcg
      781 tcaggatatc gaagaccacg agagcggcgc tgatggtctg cccctgaacg atgctgcggg
      841 gaagaaactg caccgccacg gcggcggtca ggttaatggc gatgtaagcg agaatgctcc
      901 agaggaaggc cgtgggatcg aggcctccca gacggctgcc catcaggctg gtcatgaata
      961 ctgcgagcag ggcgagaccc acgaagacgc ggtagtagtt gtagatgcgg agcaggttgg
     1021 cgttgacctc gaaccggcgc tgctcgactt cccgccttat atcctcgtgc tgaagcgtca
     1081 tccgtccgcc tgcgcgctgc ctgccgattt gcggcacgtg ctcctgagtc tagatgaccg
     1141 ccggccgttt gctgagaccg ggagcagctt tctgctcaac cgacgaaggg aagccagctg
     1201 aatatgccgc ctttcttctt cggtggcggg gcggcgtccg gattgacggg tggcggttca
     1261 aagccttcag gatgcttgat cgtgatcggc ggcgggacct tgggacggtc gagcagaccg
     1321 aacgtgacca tgttggtcca ggatcggtcg cggttgcgca cggtgctcgc cagggtcagg
     1381 ttgccgtcgg tgtcgaacgc cgggtagttc gggtagttga gcgccaatac ccgcagtgcg
     1441 tcgttggcgg cctcctccag ccccaggcgc cagttggcct caaccagtat ggccagcgca
     1501 tcgtctacgg attgggactt ggagtaggtc tcgatgacgt atctcgcccg gttgctggcc
     1561 gccagataag cgcccctgcg catgtagtag tcggccacgt gaatctccga ctctgccagg
     1621 aggttccgca gatagatcat ccgcaggttc gcgtcgggcg cgtactggct gtcggggtaa
     1681 agggccagca gctgagcgaa gtcggcgtaa gcgtcgcggg cggcggtcat gtcccgtttt
     1741 gcaagatcgg tcgcgaagat gcgatccagc aggccccggt tcttgttgaa ggcggccaga
     1801 gcctttatgt agtaggcgta gtcgatgttg gcatgctgag gatggaggcg catgaaccgg
     1861 tccgcggacg cgcgggccgt gtcgtgatcg taggacatgt actgagcgta gatgatctcc
     1921 agctgggcct gctccgcgta gcggccgaaa ggaaaccggg cctcgagctg ctggagaccc
     1981 tggatcgccc ggtcgtagtt gccggcccgg agcgcgcgtt gcgtgtcgcg gtagagctgc
     2041 tgctccgtcg cgttcaggtc ctcttcggct ttgtccttgt ccttgccgac ccagggcatc
     2101 caggagcagc cggaagacgc aaggcaaagc agtaggagag gcaaaagaaa gggcatgagg
     2161 gtgaatttgc gcatggatcc tgcagctttg gttcaggcgt gagccagaaa gtttcgctaa
     2221 tttaacgatt tagcgtacgg ttgcaaccag agcagagggc gatggcggag ccgctggaaa
     2281 tccagaaacg agtaacggtg ccacctgacg tggcgggaga gcgcgtcgac aaggcggcgg
     2341 cccggctgtt cccggaattc tcccgcgccg cgttgaccgc ctggattcag gatggggcgc
     2401 tcacggtgga cggtgcccgg gtcaaaccaa aagtgaagct cgtagggggc gaaacactcg
     2461 tgctcgatgc cgtcgtcgcg ccc
//
LOCUS       DBNHOX010000483         2617 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1195621, whole genome shotgun sequence.
ACCESSION   DBNHOX010000483 DBNHOX010000000
VERSION     DBNHOX010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1195621"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /locus_tag="AC16UZ_10635"
     CDS             <1..1469
                     /locus_tag="AC16UZ_10635"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6247181.1"
                     /translation="DQDLYNPDINRSVWGEIMEAADRHNEPGTFTAFMGWEWSSTPQG
                     QNLHRVIVMRGDKDNGDEFVPYTSLDSDRPEDLWAWLEETAEAAGTDFLAIPHNSNIS
                     GGLMFPLVNSDKEAVDAEYAKLRMRWEPVVEMTQIKGDSETHPMLSPNDEFADYETYE
                     HLIAAEGIEQQTMFTEGFLGTLSDADRAVLESQEKRIVKNGDYARTGLLRGLVLEERG
                     RGNPYKFGMIGSTDSHTAMASAEEDNFHGKMAIDSTPGTKREDIIPGTPGWDMGAAGL
                     AAVWATENTREGIFDAMKRKEVYATTGPRIELRFFGGFDFAAADAQARDLASVGYGKG
                     VPMGGDLTASPAGNAPSFLVSAVKDPVGANLDRVQIVKGWVDAEGMPQEKIFDVALAD
                     GRTVGTDGKVAPVGNTVDVSTGHYTNAIGDPQLTVVWRDPEFDPGKRAFYYVRVLQIP
                     TPRHTLYDAIALGISVEETGHPATIQERAYSSPIWYAP"
     gene            1541..2440
                     /locus_tag="AC16UZ_10640"
     CDS             1541..2440
                     /locus_tag="AC16UZ_10640"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR6247182.1"
                     /translation="MNKLLKEPLLHFLVLGLALFVLFDVVSDEKETSDSKLIRVDRET
                     LLTFIQFRTRAFEPSIAAQRLDGLSDQELKLLIDDYIREEALYREALALGMDKNDYVI
                     KRRMIQSIEFITRGFVTRAVEVSDDDVKAHYEANRNDYYVEPFVTFTHVFISGENRSE
                     AEVDALAAAELTELNARQIPFSAAPGRGERFPFFVNYVERDPEFVASHFGADMANEIF
                     ALEPDELVWRGPFLSTYGAHLVLLTRKAPGRFPPLGEIEESVRQDAERAALQKANETA
                     IQAIVDTYEVERQPEPRTRAGAG"
BASE COUNT          500 a          871 c          830 g          416 t
ORIGIN      
        1 cggaccagga cctctacaac ccggacatca accgctcggt ttggggcgag atcatggaag
       61 ccgccgaccg tcacaacgag cccggcacct tcaccgcgtt catgggctgg gagtggtcgt
      121 ccacgcctca ggggcagaac ctgcaccggg tgatcgtcat gcgcggcgac aaggacaacg
      181 gcgacgagtt cgttccctat accagcctgg acagcgaccg gcccgaggat ctgtgggcgt
      241 ggctggagga gactgccgag gcggccggca ccgactttct cgccatcccc cacaactcga
      301 acatctccgg tgggctcatg ttcccgctgg tgaacagcga caaagaagcc gtggacgcgg
      361 agtacgcgaa gctgcgcatg cgctgggagc cggtggtgga gatgacccag atcaagggcg
      421 actccgagac ccatcccatg ctctcgccga atgacgagtt cgccgactac gagacctacg
      481 agcacctcat cgctgccgaa ggcatcgagc agcagaccat gttcaccgag ggctttctcg
      541 gcacgttgag cgacgcggac cgagccgtgc tcgaaagcca ggagaagcgc atcgtcaaga
      601 atggcgacta cgcccgaacg gggctgctcc gcgggctggt gctcgaagag cgcgggcgcg
      661 gcaaccccta caagttcggc atgatcggct cgacggactc tcataccgcc atggcatccg
      721 ccgaagagga caacttccac ggcaagatgg ccattgactc cacgccggga accaagcgcg
      781 aagacatcat tcccggcacg ccgggctggg acatgggcgc ggcgggcttg gcagcggtgt
      841 gggccacgga gaacacccgc gaggggatct tcgacgccat gaagcgcaag gaggtctacg
      901 ccaccacggg gccgcgcatc gagctgcgct tcttcggggg tttcgacttc gccgccgctg
      961 acgcccaggc ccgggatctg gcctcggtgg ggtacgggaa aggggtcccc atgggcggcg
     1021 atctgaccgc gtcacccgcg ggcaacgcgc cttcgtttct ggtgagcgcc gtgaaggatc
     1081 cagtgggcgc caatctcgat cgggtccaga tcgtcaaggg ctgggtggac gccgagggga
     1141 tgccccagga gaagatcttc gacgtggcgc tggcagacgg ccgaacggta ggcacagatg
     1201 ggaaagtcgc ccccgtgggc aatacggtgg acgtctccac cggccattac accaacgcca
     1261 tcggggatcc ccagctcacc gtggtctgga gggacccgga gttcgacccc ggcaagcgcg
     1321 ccttctacta cgtgcgggtg ctgcagattc ccactccccg acacacgctc tacgacgcca
     1381 tcgcgctcgg gatatcggtg gaagagacgg ggcatccggc gaccattcag gaacgggcgt
     1441 actcctcgcc catctggtac gcaccctgac gacgtttgga ctctgacccc ccgctgccgc
     1501 ccacccggcg gcagcgagcg gcgtcatttt agagagagcc atgaacaagc ttctcaaaga
     1561 accccttctg cacttcctcg ttctggggct ggccctgttc gtgctgttcg acgtggtctc
     1621 cgacgagaag gaaacgtcag acagcaagct catcagggtg gatcgcgaga ccctgctgac
     1681 cttcattcag ttccggaccc gcgccttcga gccgagcatc gctgcccagc ggctcgacgg
     1741 cctttccgat caggagctca agctgctcat cgacgactac atccgcgagg aggccctgta
     1801 ccgcgaagcg ctcgccctgg gcatggacaa gaacgactac gtgatcaagc gccgcatgat
     1861 ccagtccatc gagttcatca ccaggggctt cgtcacccgg gccgtggagg tgtccgacga
     1921 cgacgtgaag gcccattacg aggccaatcg gaacgattac tacgtggaac cgtttgtcac
     1981 gttcacgcac gtcttcatat ccggtgagaa ccgcagcgaa gccgaggtcg acgcgctggc
     2041 tgccgccgag ctcaccgagc tcaatgcccg ccagataccg ttcagcgccg cccccgggcg
     2101 cggggagcgt ttcccctttt tcgtcaacta cgtggagcgc gacccggaat tcgtcgccag
     2161 ccacttcggg gctgacatgg cgaacgagat cttcgccctg gaacccgacg agctcgtctg
     2221 gcgtggacca tttctgtcaa cctacggcgc ccacctggtc ctgctgaccc gcaaagcgcc
     2281 cggccgcttt cccccgctcg gtgagatcga agaaagcgtt cggcaggacg ccgagcgagc
     2341 cgcgttgcag aaggcgaacg aaaccgccat tcaggccatc gtggacacct acgaggtgga
     2401 gcggcagccc gagccccgca cgcgcgccgg cgcaggatga gaactctccg ctcagccgtc
     2461 ctgggtctgt gggcgtgctg cgctgcgctg cccgccgtcg gccacgatgc gcggccgaac
     2521 tacgtccaga tcacggagac ggcgcccaat agcttcaacg tggcgtggaa ggttcctgcc
     2581 agcatgccgg gcaacgcgct gccccatcct ctgctgc
//
LOCUS       DBNHOX010000484         3687 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_745724, whole genome shotgun sequence.
ACCESSION   DBNHOX010000484 DBNHOX010000000
VERSION     DBNHOX010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_745724"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="AC16UZ_10645"
     CDS             <1..621
                     /locus_tag="AC16UZ_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247183.1"
                     /translation="VLISALHDDRRDRSVLFWKSMPVSDAETVLSKLVTVVWVAPVVV
                     IAGIVAAQLFLLIVISTLTLVEDVGGAGRLWWHSGLLTGTVELVVGYVIQGFWALPLY
                     AWLLAVSAAAPRLPILWAVLLPGLPAIAERMVLGTHGVSDFVRAHLEFAALPAAASED
                     DGIIQPAVGLAEQLSLVFSFQMWLGVVVGGLLLWLAVYLRNRNNDL"
     gene            708..2000
                     /locus_tag="AC16UZ_10650"
     CDS             708..2000
                     /locus_tag="AC16UZ_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6247184.1"
                     /translation="MAWDFSTEPEFQKELDWMDEFVREEIEPLDFIIGHPNDFSDPNR
                     AKLIPPLQEEVKKRGLWACHLGPELGGQGYGQVKLGLMNEILGRAKCAPVVFGCQAPD
                     SGNAEILAHYGTPEQKDRYLEPLLNNEICSCYSMTEPQGGADPKVFTCRAELIGDEWV
                     INGEKWFSSNARYASFLIVMAVTDPEAPPYQRMSMFIVPTETAGIEIVRHVAVGYESK
                     GAHAYIRYNDVRIPRDHMLGEPGQGFIVSQVRLGGGRIHHAMRTMGKARRAFDMMCER
                     ALSRETQGEVLAQKQLVQAMIADSWIDIEQFRLFVLRTAWKIDKLKNYKEVRADISAV
                     KAQMPKVYHDVYARALQVHGSLGLSTEMPFAAGVLDSFHMGLADGPTEVHKVTVAKQV
                     LGQYQGTDDLFPEYHLIRRKEAALAKYADIIEHEVGNA"
     gene            complement(2017..2793)
                     /locus_tag="AC16UZ_10655"
     CDS             complement(2017..2793)
                     /locus_tag="AC16UZ_10655"
                     /inference="COORDINATES: protein motif:HMM:NF018777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HIR6247185.1"
                     /translation="MIRITFLLRRKPELSAEEFYRYWREEHGPLVASHASRLNTLRYV
                     QVHTIDDPANQAMATARGGMEAPYDGVAEMWWESRAALVASLETKGGRRAGAELVEDE
                     ARFIDLPNSPMWLGHEYPQVNPTPENLVARERDTLVKLYFPLRPVQGLDERDAQSYWY
                     SNHGPIIRRQAAGVGIQRYVQVHRVADELEAGLRKARGTQVEGYMGHAELWFDRTVLG
                     VSTPERRAANERAIADESNFIDFRRSCMWLAKEHVFVDRR"
     gene            complement(2893..>3687)
                     /locus_tag="AC16UZ_10660"
     CDS             complement(2893..>3687)
                     /locus_tag="AC16UZ_10660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6247186.1"
                     /translation="IGISATGTNVGGIVFPLLIAGWLTDFGWRETFLWLAVTAAVVVG
                     PLSWFVLGRTPAGHHPATRTTPGGVSVWTTRAILSTRRFWIPGLAILPLNLAFGAVQY
                     NLGVLSRDLGLATGTAANLIALHAVCMIAGKFFFGSLGDRIDHRYLFWLSAGLMVAAL
                     LVLQGETPVWMLVTGIVMMGLAGGGILPMFGMVFGARFGVAAFGRVMGLVMTTMTIGA
                     MGPLLAGWAYDLTGSYDAAFLGMALLLLPAAVAMIWLPQPGTDTAS"
BASE COUNT          644 a         1259 c         1199 g          585 t
ORIGIN      
        1 gtcctgatct ccgccctgca cgacgaccgg cgcgaccgca gcgtcctgtt ctggaaatcc
       61 atgccggtga gcgatgccga gacggtgctc agcaagctcg tcaccgtggt ctgggtcgcc
      121 cccgtcgtcg tcatcgcggg catcgtggcc gcgcagctct tccttctcat cgtcatctcc
      181 accctgaccc tggtggagga cgtcggcggc gcggggcggc tgtggtggca ttccggcctg
      241 ctgaccggaa ccgtggagct cgtggtcggc tacgtgattc agggcttctg ggccctgccc
      301 ctgtacgcct ggctgctggc cgtgtccgcg gcggcgcccc ggctgccgat cctgtgggcc
      361 gtgctcctgc ccggcttgcc ggccattgcc gagcgcatgg tgctcggcac ccatggggta
      421 agcgacttcg ttcgcgccca tctggagttc gcagcacttc cggcggccgc cagcgaggac
      481 gacggaatca tccagcccgc cgtggggctg gccgagcagc tctcgctggt cttcagcttc
      541 cagatgtggc tgggggtcgt cgtgggcggc ctgcttctct ggctcgccgt gtacttgcgc
      601 aaccgcaaca acgacctctg aagacgacct ctgacgacga cctcggagaa cgacctgcaa
      661 agacgcctct ggtaagctcg ccttcttcac cagggaggac gcacgccatg gcatgggatt
      721 tctcgaccga gcccgagttt cagaaagagc tggactggat ggacgagttc gtccgcgagg
      781 agatcgaacc gctcgacttc atcatcggcc accccaacga cttctccgac cccaaccggg
      841 ccaagctcat tccgcccctg caggaagagg tgaagaagcg cggcctgtgg gcctgccatc
      901 tgggccccga gctcggcggc cagggctacg gccaggtgaa gctggggctg atgaacgaga
      961 tcctcggccg ggccaaatgc gcacccgtgg tcttcggctg ccaggcgccg gactcgggca
     1021 acgcggagat cctcgcccac tacggcacgc cggagcagaa ggaccgctac ctggagccgc
     1081 ttctgaacaa cgagatctgc tcctgttact ccatgacgga acctcagggc ggcgccgatc
     1141 ccaaggtgtt cacctgccgc gcggagctca tcggtgacga gtgggtgatc aacggggaga
     1201 agtggttttc ctccaacgcc cgctacgcgt cgttcctcat cgtgatggcg gtgaccgacc
     1261 cggaggcacc cccctatcag cgcatgtcca tgttcatcgt tcccacggag acggccggca
     1321 tcgagatcgt ccgccacgtg gccgtgggct acgagtccaa gggggcccac gcctacatcc
     1381 gctacaacga cgtgcgcatc ccccgggacc acatgctcgg ggagccgggc cagggattca
     1441 tcgtctctca ggtgcggctc ggcggcggcc gcattcacca cgccatgcgc accatgggca
     1501 aggcgcgccg cgctttcgac atgatgtgcg agcgggccct gtcgcgggaa acccaggggg
     1561 aagtgctggc gcagaagcag ctcgtccagg ccatgatcgc ggactcctgg atcgacatcg
     1621 agcagttccg cctgttcgtg ctgcgcaccg cgtggaagat cgacaagctc aagaactaca
     1681 aggaagtccg cgcggacatc tcagccgtca aggcccagat gcccaaggtc taccacgacg
     1741 tctatgcccg cgcgctgcag gtgcacggct ccctgggcct gtccacggag atgccctttg
     1801 ctgccggcgt gctggactcg ttccacatgg ggctggccga cgggcccacc gaggttcaca
     1861 aggtgaccgt ggccaagcag gtgctcgggc agtaccaggg taccgacgac ctgttcccgg
     1921 aatatcacct gatcaggcgc aaggaagcgg ccctggccaa gtacgcggac atcatcgagc
     1981 acgaggtggg caacgcctga gccacccgcg tcgccgtcac cggcggtcga cgaagacgtg
     2041 ctccttggcc agccacatgc acgagcgccg gaagtcgatg aagttgctct cgtcggcgat
     2101 ggcgcgctcg ttggccgccc gccgctccgg cgtggaaacg ccgagcacgg tgcggtcgaa
     2161 ccacagctcg gcgtggccca tgtaaccctc cacctgggta cccctggctt tgcgcagccc
     2221 ggcctccagc tcgtccgcca cccggtggac ctgaacgtag cgctggatgc cgacaccggc
     2281 ggcctgtcgc cggatgatgg gcccgtggtt gctgtaccag taactctggg cgtcgcgctc
     2341 gtccaggccc tgaaccggtc gtagcgggaa gtacagcttc accagggtgt cgcgctcccg
     2401 ggcgaccaga ttctccggcg tcgggttcac ctgggggtac tcgtggccga gccacatggg
     2461 cgagttgggc agatcgatga aacgcgcctc gtcctccacc agctcggcgc ccgcccgccg
     2521 gccgcctttg gtttccagag aggccacgag cgccgcacgg ctctcccacc acatctcggc
     2581 aacgccgtcg tacggcgctt ccatgccgcc ccgggcggtg gccatggcct gattggcggg
     2641 gtcgtcgatg gtgtggacct ggacgtagcg cagggtgttc agccggcttg cgtgactcgc
     2701 caccagcggg ccgtgctcct cgcgccagta acgatagaac tcctcggccg acagctccgg
     2761 cttcctgcgc agcagaaaag tgatgcgaat catgcttccc ccaagaaaaa tttcgacggc
     2821 ccctggccgc tgaaattaaa ccgcggggga tctcacatgc aaccggcacc ggccgaaatg
     2881 catgcgcacg ggtcaggacg ccgtgtcggt tccgggttgc ggcagccaga tcatggcgac
     2941 cgcagcggga agcagcagca gcgccatgcc cagaaaagcc gcgtcgtagc tgcccgtgag
     3001 gtcgtatgcc catcccgcca gcagcggacc catggcgccg atggtcatgg tggtcatgac
     3061 gagccccatc acacggccga aggcggccac gccgaaacgc gcgccgaaca ccatgccgaa
     3121 catgggcagg atcccgccac cggccaggcc catcatgacg atgccggtga caagcatcca
     3181 cacgggcgtc tcgccctgca gcaccagcag ggcggcgacc atgagcccgg cggacagcca
     3241 gaacagataa cggtgatcaa tgcggtcgcc caggctgccg aagaaaaact tgccggcgat
     3301 catgcagacg gcgtgcaagg cgatgaggtt cgccgccgtc ccggttgcca gccccagatc
     3361 gcggctgagc acccccaggt tgtactgcac ggccccgaag gccaggttca gcggcaggat
     3421 ggcgagcccg ggaatccaga agcgtcgggt tgaaagaatc gcccgggtgg tccagacgct
     3481 gacaccgccc ggggtcgtgc gggtggccgg atgatgtccg gccggcgttc gcccgagcac
     3541 gaaccagctc aggggaccca cgaccaccgc agcggtgacc gccagccaca ggaaggtctc
     3601 ccgccagccg aagtccgtca gccagccggc aatcagcaac gggaaaacga tgccgccgac
     3661 gttggtgccc gtggccgata tgccgat
//
LOCUS       DBNHOX010000485         2233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1581130, whole genome shotgun sequence.
ACCESSION   DBNHOX010000485 DBNHOX010000000
VERSION     DBNHOX010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1581130"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="AC16UZ_10665"
     CDS             <1..237
                     /locus_tag="AC16UZ_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247187.1"
                     /translation="ENEGSIWKNDRKEKDTHPDFRGEALINGVEYWVKAWRRQPGSNP
                     KAPALKFSFTEREKRQSTPAQEDEGSDPDDLIPF"
     gene            237..437
                     /locus_tag="AC16UZ_10670"
     CDS             237..437
                     /locus_tag="AC16UZ_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247188.1"
                     /translation="MNPHPRCPVCQAPVIYATSGTDYLVLDPVPVVGYQHVPEAWGIE
                     PREPWVPGVIHPEHKCPVIAEE"
     gene            437..700
                     /locus_tag="AC16UZ_10675"
     CDS             437..700
                     /locus_tag="AC16UZ_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247189.1"
                     /translation="MTDKDCPHGQQPSTCYRCELERENARLRAADQARMGDRWNLEAR
                     VAELEAAVREAASTLRNDLYAKRVSNALAILEEALARAALGEE"
     gene            complement(1462..1923)
                     /locus_tag="AC16UZ_10680"
     CDS             complement(1462..1923)
                     /locus_tag="AC16UZ_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247190.1"
                     /translation="MSDEREIPRASRVSVGFHALTLVLLGALLAIAISPQWQSLEEPV
                     IPHAQRFLETQAMAGQIVTLEIDRTRVRYCPGAVDEFWRDERFPHRVIQGHRVPASPG
                     TKLGRIVSELQKKIPETATAGRWCYTPIIRYECPDRVYEPVWTPACIEVVK"
     gene            complement(1920..2147)
                     /locus_tag="AC16UZ_10685"
     CDS             complement(1920..2147)
                     /locus_tag="AC16UZ_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247191.1"
                     /translation="MPDDTLSRTLIREVAQLQSQTEQLREEVHALRGQQAEIIAQMNR
                     WRGAVPMLLFIGGAIGTVVTSWGNIMDFFRR"
BASE COUNT          411 a          692 c          757 g          373 t
ORIGIN      
        1 gagaacgaag gctcgatctg gaagaacgac cggaaggaga aggacacgca ccccgacttc
       61 aggggggaag ccctcatcaa cggggtggag tattgggtaa aggcgtggag gcgacagcct
      121 ggaagtaacc ccaaggcgcc ggctctgaaa ttctctttca cggagagaga gaagcggcag
      181 agcactccgg ctcaagagga tgagggcagc gacccagacg atctaatccc cttctgatga
      241 acccacaccc ccgctgcccc gtctgccagg ctccggtcat ctacgccacg tctggtacgg
      301 actatctggt gctcgacccg gtgccggtgg tgggctacca gcacgtcccg gaggcgtggg
      361 gcatcgagcc tcgtgagcca tgggtgccgg gggtgatcca tccagagcac aaatgccctg
      421 tgattgcgga ggagtgatga cagacaaaga ctgcccccac ggccagcagc cgagcacctg
      481 ctaccgctgc gagctggagc gcgagaacgc acggctgcgt gccgctgacc aggcgaggat
      541 gggcgacagg tggaatctgg aggcccgcgt ggccgaactg gaggcggcgg tgcgggaggc
      601 ggctagcacg ctgcgtaacg acctatacgc caagcgcgta tcaaatgcgc tggcaatcct
      661 ggaagaggcg ctggcccggg cggcgctggg ggaggagtga tgcccctccg cgaatggcta
      721 gtcgccggca tcggcgccgc catcctgctc gggtggctgg tggtgcagtg gtgggcaggg
      781 tgatcaattg gcgtttcaag cgcttaggca agcctatggg ccggcggcct cgggtgtggg
      841 aaggcttctg cctgatgttt ctgctgaaaa cggggtatcg gctgtacggg cgcccagtcc
      901 gcagagcgct ggaaccgtgg cgttcacgac gacatcgccc acgacgacga ccaccaccgg
      961 cctatttgca attaacacga ccagtaggcc gacgacggtg gcgagagcga cgagacgcca
     1021 ggagatggac atggagccgg aggtccgaat catcggaaac gatgagctag agatcggcga
     1081 tgagttccgc gagtgcccca attgcggcgc ttgcgcgcag gtttggtctc gcggctcatg
     1141 gcgtcgaggt cgatggcatt cacaagcctg gcaatggatc tgtcctggct gcgaaagcgc
     1201 gacacggatc taccaccagc gcactctggc gatggtgatg gcgtggccga gcaaagccaa
     1261 gcaggttgga cggttcgcta tactgccatc gctctaccag aagtagtcgc cctcctccgc
     1321 cggccgggcc agccgtctgc cgctcggtgc cgctagtcgg ggtagatttg cggcaggcag
     1381 tgttcacgcg acgcgggcgt gcccgaacgg cgcgcctcgc cggcaatcga atgcggttgc
     1441 cgggtcagca ggacgggcac gttacttgac gacctcgatg caggccggcg tccacaccgg
     1501 ctcatacacc cggtccgggc attcgtaccg gattatcggc gtgtagcacc accgcccggc
     1561 ggtggcggtc tctgggattt tcttttgcag ctcgctgacg atccggccca gtttcgtccc
     1621 cgggctcgcc ggcacccgat gcccctggat cacccggtgc gggaagcgct cgtctcgcca
     1681 gaactcgtcc actgcccccg gacagtagcg cacccgcgtc ctgtcgatct cgagcgtgac
     1741 aatctgcccg gccatggctt gggtctccag gaagcgctgt gcgtgcggga tcaccggctc
     1801 ctcgaggctt tgccactgcg gcgagatcgc gatggcgagc agcgccccga gtaggaccaa
     1861 cgtcagggcg tgaaaaccca ccgagacacg tgacgcacgc gggatctcgc gctcgtcgct
     1921 catcgccgga agaagtccat gatgttgccc cacgacgtca ccaccgtgcc gatggccccg
     1981 ccgatgaaga ggagcatcgg caccgcgcct cgccagcggt tcatttgcgc gatgatctcc
     2041 gcttgttgcc cacgcagagc gtgtacctcc tcgcgtagct gctccgtctg ggactggagc
     2101 tgagccacct cgcggatgag cgtgcgggaa agggtgtcat ctggcacggc atcatggcct
     2161 gtcgtagtgc acggcacacc gcgtgccgtt gtacgtgagc tgtaagccgt ccgggagcgt
     2221 gccagacact gcc
//
LOCUS       DBNHOX010000486         2515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_231947, whole genome shotgun sequence.
ACCESSION   DBNHOX010000486 DBNHOX010000000
VERSION     DBNHOX010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_231947"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="AC16UZ_10690"
     CDS             <1..449
                     /locus_tag="AC16UZ_10690"
                     /inference="COORDINATES: protein motif:HMM:NF019159.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR6247192.1"
                     /translation="LDRLAADTHPLLNLEPRDTVLFSSRVIPGNEPAVEKLIRRLERF
                     GVTVIQDSELNMPIHASGHPARDELRDLYRWVRPDVALPVHGEEAHMQANAELAREVG
                     VPRQMTGRNGDLFMLAPVRGVRRDAARVGRLGLDNGRLVHLGPPRR"
     gene            457..1134
                     /locus_tag="AC16UZ_10695"
     CDS             457..1134
                     /locus_tag="AC16UZ_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6247193.1"
                     /translation="MKIHADLSRRAVVKSPELPWVPSPLPGVDRRMLERDGGEIARAT
                     SIVRYAPESFFAQHEHSGGEEFFVLDGVFSDESGDYPAGWYVRNPIGSRHRPHTQSGT
                     TILVKLRQFQIGDDEYVRTDTNSGSWSPGEAEGVTIMALHAFGTERVSLARLATGTRM
                     PERAYPEGAEMFVLEGEIRDEHGVYPEGTWIRSPAGFSHRLRSPSGARLLVKTGHLPA
                     APASDVR"
     gene            1334..1723
                     /locus_tag="AC16UZ_10700"
     CDS             1334..1723
                     /locus_tag="AC16UZ_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6247194.1"
                     /translation="MTRFRTALALAVTLSAAAGCSERPADGPAAVRADDSPASLQSPP
                     AGQAAYAMACASCHDTGVAGAPRIGDDQSWSNRSPLWTAVLAEHAEDGYLGMPAMGGA
                     EELSERAVQDAVEYMLSATFPEAPPAD"
     gene            complement(1746..>2515)
                     /locus_tag="AC16UZ_10705"
     CDS             complement(1746..>2515)
                     /locus_tag="AC16UZ_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR6247195.1"
                     /translation="GPDGLDSRQYGAREQAYSAGLEDGARGLRVGVLSEGFGREDSEA
                     DVDAKVRAAAERFGRLGADVSEVSVPLHAVASAICFSVIQSTVETIFGHDGALISRLD
                     PAVASLVAAQHAWRDRADELPANVKVMLMSAEYLRREHGWEYVAKGVNQIRRLRAAYD
                     AALAEVDLLLLPTTPMKATELPAPDADPATVLGLAFAPLSNTMAFNNTHHPAISVPCG
                     MSDGLPVGMMLVGGHFEESLLYRAAFAFEQHEDWREL"
BASE COUNT          415 a          877 c          826 g          397 t
ORIGIN      
        1 cgctggaccg gctggccgct gacacgcacc cgctgttgaa tctggaaccc cgggacaccg
       61 tcctcttcag ctcccgggtc attcccggca acgagccggc cgtggaaaag ctcatccgcc
      121 ggctcgaacg ttttggcgtc acggtgattc aggacagcga gctcaacatg ccgatccacg
      181 catcggggca tcctgcccgg gacgagctgc gcgacctcta ccgctgggtg cggccagacg
      241 ttgccctccc cgttcacggg gaggaggcgc acatgcaggc aaacgcggag ctggcgaggg
      301 aagtcggcgt gccgcgccag atgaccgggc gtaacggcga cctcttcatg ctcgcgcccg
      361 ttcggggcgt gcggcgggac gccgcccggg tgggccggct cggcctggac aacgggcgcc
      421 tggtccacct gggcccgccc cgccggtgat ggcgccatga agatccacgc ggatctgagc
      481 cgacgggcgg tggtaaaaag cccggaactg ccttgggtgc cttctccgct gccaggggtg
      541 gatcgacgca tgctcgagcg ggacggtggt gagatcgccc gggcgacgtc catcgtccgc
      601 tacgcgccgg aatctttctt cgcccagcac gagcactcag gcggggaaga gtttttcgtt
      661 ctcgacgggg tcttttcgga cgagtcgggc gactacccgg ccgggtggta cgtccgcaac
      721 cccattgggt ccagacaccg tccccacacg cagagcggca cgactatctt ggtgaagctg
      781 cggcagtttc agattggcga cgacgagtac gttcgcacag acaccaactc cggcagctgg
      841 tcgccaggag aggccgaggg cgtcacgatc atggcgctgc acgctttcgg caccgagcgc
      901 gtgagccttg cgcggctggc tacaggtacc cggatgccag agcgcgccta ccccgagggc
      961 gcggagatgt tcgtcctcga aggcgagatc cgcgacgagc acggcgtcta tcccgagggc
     1021 acgtggatcc gcagcccggc aggcttctct caccggctga ggtcgccctc gggcgcccgg
     1081 ctactcgtga agaccgggca tctgccggcc gcgccggcgt cagacgtcag gtaagccgcc
     1141 ttgcccacct gctccaccaa gacgtcccga gcgccttaga gatcggcgat gcctttgccg
     1201 tccttgaagt tgcaggcgta gaactttctg accagtctgg tttctgccgc gccaggaggc
     1261 tcggtcaaac gcccgcttgc gcgccggccg catgatactt agcctttact caacgaccag
     1321 tcgagaaacc gccgtgacca gatttcgcac cgcccttgct cttgccgtca cgctgtccgc
     1381 ggcggccggc tgctccgaac gtccggcaga cgggcccgcc gccgtccgag ccgacgactc
     1441 tccggcgtcg ctccaatcgc cgccggccgg tcaggccgcg tatgcgatgg catgtgcgag
     1501 ctgccacgac acaggggtcg cgggcgcgcc ccgcatcggg gacgatcaga gctggtcaaa
     1561 ccgctcaccg ctctggacgg ccgtgctggc cgagcacgcc gaggacggct atctgggaat
     1621 gcccgccatg ggcggtgccg aggaactcag cgagcgggca gtgcaggacg ccgtcgagta
     1681 catgctctcg gccacctttc cagaggcacc cccggcagat tgacccctgc gcctgaccgg
     1741 ggcgctcaaa gctcgcgcca gtcctcgtgc tgctcgaagg cgaaagcggc gcggtagagc
     1801 agggactcct cgaagtggcc gcccaccagc atcatgccca cgggcagccc gtcactcatg
     1861 ccgcagggca cggaaatcgc cgggtgatgg gtattgttga acgccatggt gttgctcagc
     1921 ggggcgaatg ccagcccgag cacggtggcc gggtcggcgt ccggtgccgg cagctcggtg
     1981 gctttcatgg gcgtggtcgg caacaacagc agatccactt cggccaacgc cgcatcgtag
     2041 gccgcccgca accggcggat ctgattgacg cctttggcca cgtattccca gccgtgctcg
     2101 cgacgcagat attcggccga catcaacatc accttgacgt ttgcaggcag ctcgtcggcc
     2161 cggtcgcgcc aggcgtgctg agcggccacc aggctggcaa cggcaggatc caggcggctt
     2221 atcagagcgc cgtcatgacc gaatatggtt tccacggtgg actgaatcac cgaaaagcag
     2281 atggccgaag ccaccgcgtg gagcggcacg gaaacctcac tgacgtccgc ccccaggcgg
     2341 ccgaagcgct cggcggccgc gcgcaccttg gcatccacgt ccgcttctga atcctcgcgg
     2401 ccgaacccct cgctcagcac gcccacgcgc aggccccgcg caccgtcttc cagcccagcc
     2461 gagtatgcct gctcccgcgc tccgtattgc cgactgtcca ggccgtccgg gccag
//
LOCUS       DBNHOX010000487         1826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_939056, whole genome shotgun sequence.
ACCESSION   DBNHOX010000487 DBNHOX010000000
VERSION     DBNHOX010000487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_939056"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="AC16UZ_10710"
     CDS             complement(<1..807)
                     /locus_tag="AC16UZ_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6247196.1"
                     /translation="MNHWMVGDVKITRVVEMEVAGGTRFILPDATRDACLPIEWLHPH
                     FMDEAGNLIMSIHALVVDTGSRRIIVDTCIGNDKQRSIPAWTNLQTSFLEDLGAAGYP
                     RETVDTVLCTHLHVDHVGWNTMLVDGEWVPTFPNARYLVAEKEFRYWDGEAEDEINQG
                     IMADSVRPIVAAGLMDFVDWEHQLCPEVALEPTPGHTPGHVSVHIRSNGKEALITGDC
                     IHHPCQMTRTDWCSSADYDRAAGEATRERLLESYVDSDVLIIGTHFATPTA"
     gene            929..>1826
                     /locus_tag="AC16UZ_10715"
     CDS             929..>1826
                     /locus_tag="AC16UZ_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6247197.1"
                     /translation="MGPIAKAVDVVRALSNVPTLLALKRGMKPRPVDEHDCFAANVER
                     NAAAFPDRTAIIFEGNSVTWAEFNAQCNRFASAFKAQGLRKGDAVSVMMENRIELLAT
                     IVALNKLGVVAGLINTNLRGRPLAHCITVTESRTCVFGEEVGEAVAEVKDELPLEEGS
                     GYLFVPDAGNGAAPNWAADLSRLAADADDADPPETGDVQLGDRALYIFTSGTTGLPKA
                     AVLSNRRFVATAALVHKAGLRCSEEDRIYICLPLYHGTGLLIGVGASFSSGASMFIRR
                     KFSASNFLREVREYQTSCLIYIG"
BASE COUNT          331 a          588 c          579 g          328 t
ORIGIN      
        1 ggcggtcggc gtcgcaaagt gggtcccgat gatcaggacg tcggagtcca cgtaggattc
       61 caggagccgc tcccgggtcg cttcaccagc agcacggtcg tagtcggccg aggagcacca
      121 gtcggtccgc gtcatctggc agggatggtg gatgcagtca ccggtgatga gcgcctcctt
      181 gccgttggat cgaatgtgca cgctgacgtg cccgggcgta tgaccgggcg tcggctccag
      241 agcgacctcg ggacacagct gatgctccca gtccacgaag tccatcaggc cggcggccac
      301 gatgggccgc acggaatctg ccatgatccc ctggttgatc tcgtcctcgg cttcgccgtc
      361 ccagtagcgg aactccttct cggccaccag gtagcgggcg ttcgggaacg tgggcaccca
      421 ctcaccgtcc acgagcatgg tgttccagcc cacgtgatcc acgtgcagat gcgtgcacag
      481 cacggtgtcc acggtttcgc gcggatagcc cgcagcgccg agatcttcga gaaagctggt
      541 ctgaaggttg gtccaggcag gtattgaccg ctgcttgtcg ttgccgatgc aggtgtcgac
      601 gatgatgcgg cgcgagcccg tatccacgac gagcgcgtgg atgctcatga tcaggttccc
      661 cgcttcgtcc atgaagtgcg ggtgaagcca ctcgatgggc aggcaggcat ctctggtcgc
      721 atccggcaga atgaagcggg tgccaccggc cacctccatt tccaccacac gcgtgatctt
      781 cacatcaccc accatccaat ggttcatcgc cgctccttgt ctgcccgggc cgaactgagc
      841 ctggttgagc cccgagcatg ccacaggtcg cttgatggtg gcatacccgc aggataaggt
      901 gccccacgta gccgaacgag gagaaagcat gggcccgatc gccaaggccg tggacgtcgt
      961 ccgggcgctg agtaacgtgc ctacgctgct tgctctcaag cgcggcatga agccgaggcc
     1021 cgtggacgag cacgactgct tcgccgcgaa cgtggagcgc aacgccgccg cgttcccgga
     1081 cagaaccgcc atcatcttcg aaggcaactc ggtaacctgg gccgagttca acgctcagtg
     1141 caaccgcttt gcgtcggcat tcaaggctca gggcctccgc aaaggcgacg cggtcagcgt
     1201 gatgatggag aaccgcatcg aactgctggc gaccatcgtg gcgctcaaca agctgggcgt
     1261 ggtggccggc ctgatcaaca ccaacctacg cggccgaccc ctggctcact gcattacggt
     1321 gacggagtcg cggacttgcg tgtttggaga agaggtgggc gaagccgtcg ctgaagtgaa
     1381 ggacgagttg cccctggaag agggctccgg ctacctgttc gttccggatg caggtaacgg
     1441 cgcggcacca aactgggcgg ccgatctcag ccgccttgcg gccgacgccg acgacgccga
     1501 ccccccggag acgggcgacg tgcagcttgg cgaccgggct ctgtacatct ttacctccgg
     1561 caccaccgga ttgccaaaag cggccgtact ttccaatcgc cggttcgtcg ccaccgccgc
     1621 gctcgtccac aaagccgggc tgcgctgcag cgaggaggac aggatctata tctgtcttcc
     1681 cctctaccac ggcacggggc tgctgatcgg cgtaggcgct tcgttctcgt ccggcgcaag
     1741 catgttcatt cgtcgaaagt tctcggccag caactttctg cgcgaggtcc gcgagtatca
     1801 gaccagctgc ctgatctaca tcggcg
//
LOCUS       DBNHOX010000488         1725 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_681661, whole genome shotgun sequence.
ACCESSION   DBNHOX010000488 DBNHOX010000000
VERSION     DBNHOX010000488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_681661"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1392
                     /gene="istA"
                     /locus_tag="AC16UZ_10720"
     CDS             205..1392
                     /gene="istA"
                     /locus_tag="AC16UZ_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HIR6247198.1"
                     /translation="MLTQEDYWMIQELHERGVYQIDIADRLGVYPKTVSRALKRGGAP
                     SRTRDREKYAKLRPFMARVDELLSKDVWNAVVIYRDIQERGYTGKIRSLRQYIEPKRP
                     LRQSKATVRFETEPGQQLQHDWGEKTVQVGGRPQKIFIAVNSLGYSRRFHVMAAPRAD
                     AEHTYESLVQAFAWFGGVTHEVWVDNQKSAVIKHVPGAVRFNERFKQLARHYGFVPKA
                     CRPYRARTKGKVERMVGYVKDHFFQRYRSFESLAHLNQLLEQWLLEEADQRVHGTVRE
                     VVAERFERERPALSPLPVHRFNTSYIETRQVGWYAYVDVRGNRYSVPAACCGQAVTVH
                     VSLDEALTVYHGDRCVARHRLSTADSGWQTVPEHHQRLWAEVSVQTRSLAAYEEVGYA
                     AAG"
     gene            1376..>1725
                     /locus_tag="AC16UZ_10725"
     CDS             1376..>1725
                     /locus_tag="AC16UZ_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6247199.1"
                     /translation="MQPLDSLFEALKLDHLAQQVDALLEQAAKKDLGYREMLTEALTC
                     EWQGRKQKGTETRLAHARLPWLKTLEQFDFTFQPSIDRKVVRELGTLGFVGRAENVVL
                     LGPPGVGKTHLAIGL"
BASE COUNT          323 a          517 c          575 g          310 t
ORIGIN      
        1 atagtcgttg agcccgttga ccttggtggt ctggatcggc gagtagaggt tggcgctggc
       61 tttgaccccg ccctggttgt cggagaacgg tcatgctttt cgttgtcacc ggcatatgta
      121 aattaccgga gaacggcatt ttgaggtgtc ccctgagcaa gtcatgcgac gattccaact
      181 ccttggcggg aactggagtc gagcatgctc actcaggagg actattggat gatacaagag
      241 ctccacgaac gaggggttta tcagatcgac attgctgacc gtcttggggt ataccccaag
      301 acggtcagcc gcgcgcttaa gcgcggcggg gcgccgtcgc gaactcggga ccgggagaag
      361 tacgcgaagc tgcggccctt catggcccgc gtcgatgaac tgctgagcaa ggatgtctgg
      421 aatgcggtag tgatctaccg cgacatccag gaacgcggat acacgggcaa gatccgcagt
      481 ctgcgccagt acatcgagcc gaagcgccca ttgcggcagt cgaaggccac ggtccggttc
      541 gagaccgagc cgggccagca gctgcagcac gactggggcg agaagactgt gcaggtgggc
      601 ggtcgtccgc agaagatctt cattgcagtc aacagcttag ggtactcacg gcgcttccac
      661 gtgatggccg caccgcgagc cgacgccgag cacacctacg agagcctggt gcaggccttc
      721 gcctggttcg ggggcgtcac ccacgaggtc tgggtcgaca atcagaagtc ggcggtcatc
      781 aagcacgtgc cgggcgcggt gcgcttcaac gagcgcttca agcagctcgc ccggcactac
      841 ggcttcgtgc ccaaagcgtg ccgtccgtat cgggcccgga ccaagggcaa ggtggagcgg
      901 atggtcggct acgtgaagga ccacttcttc cagcgctatc ggtcgttcga gagcctggcg
      961 cacctgaacc agctgcttga acagtggctg ctcgaggaag ccgatcagcg tgtccatggc
     1021 accgttcggg aggtggtggc ggagcgcttc gagcgagaac gccccgcact ctcaccgttg
     1081 ccggtccatc gcttcaatac cagctacatc gagacccgcc aggtaggctg gtatgcctat
     1141 gtcgacgtcc gcggcaatcg ctactcggtg ccggcagcct gctgcggcca ggcggtcacc
     1201 gtgcacgtca gcctggatga ggcgctgacc gtctaccacg gcgatcgttg cgtcgcccga
     1261 caccggttgt cgacagccga ctctggctgg cagacggtcc ctgagcatca ccagcgcctg
     1321 tgggccgagg tctcggtgca gacccggtct ctggctgcct acgaggaggt gggctatgca
     1381 gccgctggat agcctgttcg aggcgctgaa gctggatcac ctggctcagc aggtcgatgc
     1441 gctgctggaa caggcggcga agaaggatct gggctatcga gaaatgctga ccgaggcgtt
     1501 gacctgtgaa tggcagggcc gcaagcagaa gggtaccgag acgcggctgg ctcatgcacg
     1561 cctgccgtgg ctcaagacgc tggagcagtt cgacttcacc ttccagccct ccatcgaccg
     1621 caaggtggtg cgggaactcg gcacgctcgg cttcgtcggc cgggccgaga acgtggttct
     1681 gctcggcccg cccggggtag gcaaaaccca cctggccatc ggcct
//
LOCUS       DBNHOX010000489         1650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1773851, whole genome shotgun sequence.
ACCESSION   DBNHOX010000489 DBNHOX010000000
VERSION     DBNHOX010000489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1773851"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..673
                     /locus_tag="AC16UZ_10730"
     CDS             161..673
                     /locus_tag="AC16UZ_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247200.1"
                     /translation="MPALDDRSLGWLRYLHRKATTPDNWDRDGRPHPDRYRPIVPAGL
                     WGEYDVSGWTANGIEPYGMQPDPVAADGMLFYKGFFNLLMSLHRYVSGSTKWDQPFEM
                     IRDEANTFEWTHSGADLYYDPVADEHVGTGWSMQTPGRETVPLTGENNRVNVQLTTDN
                     RTVIVRRSVG"
     gene            773..1093
                     /locus_tag="AC16UZ_10735"
     CDS             773..1093
                     /locus_tag="AC16UZ_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247201.1"
                     /translation="MPLHIAKYERRDHTDRTDLALAALEACRSARATAGVNSSRFYWV
                     NPDEIAIVTDAEPGAWGQGSGNQPEPRAMKAMFELADLARNTSVETWADARSGEETFK
                     ISQS"
     gene            1126..>1650
                     /locus_tag="AC16UZ_10740"
     CDS             1126..>1650
                     /locus_tag="AC16UZ_10740"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6247202.1"
                     /translation="MNDIVKHPLAMDGKDPRNLRGDPITPERYYSRDYMQQEWDNLWT
                     KIWHIAGREKQLAEPGDFIVHDFMRESVIIVRQRDGSLRGFYNACGHRAQRLVWNHGH
                     QESFLCPYHGWQWGLDGSLEDLPDRDDYPQGDPVGKLGLIEVRVDTWAGLVWYTMDDE
                     APDLMKYLEPLPEIY"
BASE COUNT          334 a          554 c          507 g          255 t
ORIGIN      
        1 cggctcgacc cgacccggcg cctgatcaga aacatcatgc gcaccccgtc cgttgcggga
       61 acccggcctt cgtccgacat gagcatcctc cccacccgtc gcttggctgc agcgtagcac
      121 cattgctgcc agcttacccg cattggaccg ggagctgccg atgcctgcac tggacgaccg
      181 ctcgttaggc tggctgcgct atctgcaccg caaggccacc acacccgaca actgggatcg
      241 cgacggccga ccccacccgg accgttaccg tcccatcgtc ccggccgggt tgtgggggga
      301 gtacgacgtg tcgggctgga cggccaacgg catcgagccc tacggcatgc aaccggatcc
      361 cgtggccgca gacggcatgc tcttctacaa aggctttttc aacctgctga tgagcctgca
      421 ccgctacgtg tccggcagca cgaagtggga tcagccgttc gagatgatcc gcgacgaggc
      481 caacaccttc gagtggacgc actccggcgc cgacctctac tacgacccgg tggccgacga
      541 gcacgtgggg accggctgga gcatgcaaac cccaggccga gagaccgtgc ccctgacggg
      601 cgaaaacaat cgcgtgaacg ttcagctgac gaccgacaac cgcacggtga tcgtgcgccg
      661 gtctgtcggc tgaggtccga cctggccctg ccgggcgttc gccccggggt tccaaaccgc
      721 cttcattcgg gccattatcc ggagacggga cagaaaggat caggggaaaa ccatgccatt
      781 acacattgca aagtacgaac gccgagacca taccgaccgt accgacctgg cacttgccgc
      841 gctggaagcc tgccgcagcg cccgggccac ggcgggggtc aacagcagcc gcttttactg
      901 ggtaaacccg gacgagatcg ccatcgtcac cgacgccgaa ccgggcgcct ggggccaggg
      961 ttccggcaac cagcccgagc cgagagcgat gaaggccatg ttcgagctcg ccgatctggc
     1021 gcgcaacacg tcggtggaaa cctgggcaga cgcccgctcc ggcgaggaga cgttcaaaat
     1081 ctcacaatcc tgatcacacc cggagccaaa acgaggggat atccgatgaa cgacatcgtc
     1141 aagcatccac ttgccatgga cggaaaagat ccgcgcaacc tgcgcggcga tcccattacg
     1201 ccggaacgct actactcccg cgattacatg cagcaggaat gggacaacct gtggacgaaa
     1261 atctggcaca tcgccggacg cgagaagcag ctggcggagc ccggggactt catcgttcac
     1321 gacttcatgc gcgagtcggt gatcatcgtt cgccagcggg acggctccct gcgcgggttc
     1381 tacaacgcct gcggacaccg cgcccagcgg ctcgtgtgga accatggcca ccaggagagt
     1441 ttcttgtgcc cctatcacgg ctggcagtgg gggcttgacg ggtctctgga ggacctgccc
     1501 gaccgggacg actatcccca gggagacccc gtgggcaaac tgggcctgat cgaggtgcgc
     1561 gtggatacct gggccgggct cgtctggtac accatggacg acgaagcgcc ggacctgatg
     1621 aagtatctcg agccccttcc ggagatctac
//
LOCUS       DBNHOX010000490         2454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1067665, whole genome shotgun sequence.
ACCESSION   DBNHOX010000490 DBNHOX010000000
VERSION     DBNHOX010000490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1067665"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1836)
                     /locus_tag="AC16UZ_10745"
     CDS             complement(<1..1836)
                     /locus_tag="AC16UZ_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR6247203.1"
                     /translation="MAERISRRRKALGLLGLAVVLALAALTAYLVYLDRTITTTFEGR
                     RWTEPAVIYARPLELHPGAALTTAEVIAELDRLGYALQPSPPKPGAYHRSGDELDVYL
                     RAFDFPNGSRDAQRIMLKFSAGSLARVQDALGRPVPLIRLDPVTIGSFFPSHGEDRLV
                     LPPERVPDLLRETLKVVEDRNFDRHAGFDLRAIARAFWVNVTAGEVRQGGSTLTQQLV
                     KSYFLDNRRTIGRKLRELAMAIILDARFGKEDLLNAYVNEIYLGQDGRRAVHGFGLGA
                     QFYFNRPLTELDIQQLALLIAVIRGPSYYDPFRHPERARARRDLVLDKMLQGGLVDED
                     QHARAVRSPLTTVAGTRRGGAYYPAFLDLVRANLTALDRDALTTRGLAVFTTLEPQTQ
                     DDLEQAVAQALGDLESSRGLEPGSLQAAAVMTATQTGEVLALSGARSAGSDGFNRALN
                     ARRPVGSLLKPVVYLAALEQGLHLASLVDDAPVVLPLAGGTTWQPENFDHVTHGEVPM
                     IRALAESLNLATVNLGLEVGVGTVAARFETLIGRPPANTYPSLLLGAEAMTPVEVSAL
                     YGIFASGGFYQPPKAVISVLDESRSPTLRRPLTLEQRIDTKTARAL"
     gene            complement(1869..>2454)
                     /locus_tag="AC16UZ_10750"
     CDS             complement(1869..>2454)
                     /locus_tag="AC16UZ_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024937.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HIR6247204.1"
                     /translation="PAQLEIVQDPLVILAAGFMYCVEFMADKIPGVDTGWDALHTLVR
                     IPAGALLAAGALGDVSPALAVTGGILGGGVAAATHATKAGSRALINTSPEPFSNWGAS
                     ILEDLAVIGGLWAALTHPMIFLAAFTIFMLLLCWLLPRIWRGVRRVFRTVGGWLGLVD
                     ASPANLQAELMKLGEAGVLTAAEVHNARRRLHAA"
BASE COUNT          413 a          786 c          874 g          381 t
ORIGIN      
        1 gagggctcgc gcggttttcg tgtcgatgcg ctgctcgagc gtgagggggc gcctcaacgt
       61 ggggcttcgg gattcgtcga gcacgctgat cacggctttg ggcggctggt agaagccgcc
      121 gctggcgaag atgccgtaga gcgccgatac ctccaccggc gtcattgcct ccgcaccgag
      181 cagaagcgaa ggatacgtgt ttgcgggtgg gcggccgatg agagtctcga agcgggcggc
      241 caccgtcccc acgcccacct cgagtcccag gttgaccgtg gcgagattca gcgactcggc
      301 cagtgcccgg atcatgggga cttctccgtg ggtcacgtga tcgaagtttt cgggttgcca
      361 ggtggtgccg ccggcaagcg ggagcacgac gggcgcgtcg tcgaccaggc tggccagatg
      421 aagcccctgc tccagcgccg ccagatagac cacgggcttg agcagggacc cgaccggccg
      481 cctggcattg agcgcccggt tgaaaccgtc gcttcccgcg ctgcgcgcgc cgctcaaggc
      541 cagcacctcg ccggtctggg tggcggtcat gacggcggcc gcctgcaggg agccgggttc
      601 cagcccccgg ctggactcca gatccccaag agcctgagcc accgcttgct ccaggtcatc
      661 ctgggtctgg ggctcgagcg tcgtgaagac cgcaaggccc cgggtggtca acgcatcgcg
      721 gtcgagggcg gtcaggttgg cgcggaccag atccaggaag gccggatagt aggccccgcc
      781 gcggcgggtg cccgcgaccg tggtcagggg tgatcgcact gcccgtgcgt gctggtcttc
      841 gtcgacgagg ccgccctgaa gcatcttgtc gaggaccaga tcccggcgcg cccgcgcccg
      901 ctcgggatgt cgaaaagggt cgtagtagga ggggcctctg atcaccgcga tgagcagcgc
      961 aagctgttgg atgtccagct ctgtcagggg acggttgaag tagaactggg cgccgagccc
     1021 gaagccgtgg acggcgcggc gcccgtcctg acccaggtaa atctcgttga cgtaggcgtt
     1081 gaggagatct tccttgccga agcgggcgtc caggatgatc gccatggcga gctcacgcag
     1141 cttgcggccg atggtgcgcc ggttgtcgag gaaatagctc ttcaccagct gctgggtcag
     1201 cgtgctgccc ccctgtctga cctccccggc ggtgacgttt acccagaacg ctcgggcgat
     1261 ggcacgcagg tcgaaaccgg cgtggcggtc gaagttgcga tcctctacga ccttgagggt
     1321 ttcacgcagc agatcgggta cccgctcggg cgggagcacc aggcggtcct cgccgtggga
     1381 aggaaagaag ctgccgatgg tgaccgggtc cagccggatc aagggcaccg gacggccgag
     1441 ggcgtcctga acccgcgcca gggacccggc agagaatttg agcattatcc gctgggcgtc
     1501 ccgggatccg ttgggaaagt cgaaggcgcg caggtaaacg tccagctcgt ctccgcttcg
     1561 gtggtaagcg ccgggtttcg gcggggatgg ctgcaacgcg tagcccagcc ggtcgagttc
     1621 ggcgatcacc tcggcggtgg tgagcgcagc gccgggatgg agctccaggg gccgggcgta
     1681 gatgacggcc ggctccgtcc agcgtcgacc ttcgaaggtg gtggtgatcg tccggtccag
     1741 atagaccaga taggctgtga gtgccgccag cgccagcaca acggcgagac ccagcagacc
     1801 cagggctttg cgtcgacggg aaatccgttc agccatcgag gcgcgggatc acgaccggcg
     1861 gcccgatgtc acgcagcgtg aagcctgcgc cttgcgttgt gaacttccgc cgcggtgaga
     1921 acgcccgcct cgcccagctt catcagctcc gcctgcaggt tggcgggcga ggcgtcgacc
     1981 aggcccagcc agccgcccac cgtgcggaac acgcgccgca ccccacgcca tatcctgggt
     2041 aacaaccagc atagcaacaa catgaaaatc gtgaaggcgg ccaagaatat catggggtgc
     2101 gtgagcgccg cccagaggcc gccgatcacg gcaaggtcct cgaggatgga agcgccccag
     2161 ttgctgaacg gctcggggga ggtgttgatc agcgcccgcg accccgcctt ggtggcatgg
     2221 gtcgccgcgg ccaccccgcc accgaggatg cctccggtca cggccagtgc gggcgacacg
     2281 tcgccgagcg cgccggctgc caggagggcg ccggcgggaa tcctgaccag ggtgtgcagc
     2341 gcatcccacc ccgtgtccac gccggggatc ttgtcggcca tgaactccac gcagtacatg
     2401 aacccggcgg ccagtatcac cagggggtcc tggacgattt cgagctgcgc cgga
//
LOCUS       DBNHOX010000491         4074 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_810518, whole genome shotgun sequence.
ACCESSION   DBNHOX010000491 DBNHOX010000000
VERSION     DBNHOX010000491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_810518"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="AC16UZ_10755"
     CDS             <1..205
                     /locus_tag="AC16UZ_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247205.1"
                     /translation="AARFGASGLEQLLAHPQGREVWQQLNESLFSPQPGPRVTGQDVI
                     KAAALTRGRRKPSGPEALPELYA"
     gene            complement(221..2893)
                     /gene="pepN"
                     /locus_tag="AC16UZ_10760"
     CDS             complement(221..2893)
                     /gene="pepN"
                     /locus_tag="AC16UZ_10760"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HIR6247206.1"
                     /translation="MSSNVPQPVFLRDYEPPSYRTLDTRLSFDVRDGYTRVESQLSVE
                     RRAGGTDTVLRLDGQNLELLSVALDGRLLGGNEFVVDAESLTLFDLPERCEITIVTRI
                     EPEKNTALEGLYKSGSMYCTQCEAEGFRRITYYQDRPDVLARFTTTLEADASLYPVLL
                     ANGNPISDVPAGEGRRRVTWEDPFPKPSYLFALVAGDLAVMEDEFVTASGRRVALRIF
                     SEPQNIGQCGYAMEALKRAMRWDEETFGREYDLDILMIVAVEDFNMGAMENKGLNIFN
                     TKCVLASPDTATDAAHLRVESVVAHEYFHNWSGNRVTCRDWFQLSLKEGFTVFRDEEF
                     SSDMNFRAVKRIEDVNFLRSSQFPEDAGPMAHPVRPDSYIEISNFYTTTVYEKGSEVV
                     RMLRTLLGEERFRRGSDLFFDRYDGCAVTTDDFLGAMADVTALDLKQFRRWYEQAGTP
                     RLQVEEGWHDGELSLRIRQSCPPTPGQGSKKPFHLPVLIGLLDGAGRDLVDGGLDCSS
                     DAQVDVREGDRSLLVHVRDEITEIRIPGLPGRPAVSFLRGFSAPVRVEYPRDPETLAF
                     LALHDSDGFARWDALQTLVVREIDAMRSGGEPAAELVALLGNFIDPALAGGASAEQRF
                     LLSELLHVADEGYLFQALDDFEVEAVCDARDRLVVSVAANHAERWRALYEANAASGAY
                     APEPDGMSRRALRNTALGYLARVETGSALESLLEDHYRSADNLTDRSAALRAVADVDG
                     LSDSFRSEMLDEFHRRWRAHALIVDLWFSVQASSPRSSAGTIRELESHPDFDGLNPNK
                     LRALHGAFGQMNHRNFHAQDGSGYDLLADRVLELDRNNPQIAARLLTPLTRWRRYDTH
                     RQALMTAALRRIEDQEGLSKDVFEVVTKTLATED"
     gene            complement(2935..3813)
                     /locus_tag="AC16UZ_10765"
     CDS             complement(2935..3813)
                     /locus_tag="AC16UZ_10765"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6247207.1"
                     /translation="MLLPLWLCFMRVIETDLDEDLSVLSRLLWERRVRHRIYEERGRQ
                     IVELADGAQAALVRDVYEALASGSLVVEAQPLPQRRPGLLAQIRRYPGLAVLVALAVA
                     LFPFTMPLAEGRVTYVASWLTIVDLIRLGSAPGTLVELVAEAAVWRWFAPMLIHFNAV
                     HLLFNCAVVIEMGRRIESRLGALWFWAIVLVVSLVANLGQYAMNANPLFGGLSGVAFG
                     LLGFVLAAGRLAPAVPQWQVPTAVAGGLLFFLVLFSTGVTEYFGLHVANAAHWFGLAA
                     GAVLGIVTGRLVGSGR"
     gene            3812..>4074
                     /locus_tag="AC16UZ_10770"
     CDS             3812..>4074
                     /locus_tag="AC16UZ_10770"
                     /inference="COORDINATES: protein motif:HMM:NF014662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR6247208.1"
                     /translation="MQAYQIGATDEMHAVDEEVLNQVTEWLLADRPCWLATVVATWGS
                     SPRPVGSLLACNAEGQIVGSLSGGCVEDDLLEKLTSGELAAEHA"
BASE COUNT          785 a         1358 c         1293 g          638 t
ORIGIN      
        1 cgccgcgcgc ttcggagcct ccggacttga gcagctgctg gcgcaccccc aggggcggga
       61 agtctggcag cagctcaacg aatctctctt ctcgccgcaa cctggcccgc gagtcaccgg
      121 ccaggacgtc atcaaagccg ccgccctgac ccggggacgg cgcaaaccct ccgggcccga
      181 agcgcttccc gagctgtacg cctgaccggc ccggggcagg tcagtcttcg gtcgccagcg
      241 tcttggtgac cacctcgaat acgtccttgg acagcccttc ctgatcctcg atcctgcgca
      301 atgccgcagt catcagcgcc tgacggtgag tgtcgtagcg tcgccagcgc gtgagcggcg
      361 tcagcagccg cgcggcgatc tgcgggttgt tccggtcgag ttccagcacc cgatccgcca
      421 ggaggtcgta gccggatccg tcctgggcat ggaaatttcg atggttcatc tggccaaacg
      481 ccccatgcaa agcgcgcagc ttgttgggat tcaggccatc gaagtcaggg tgcgattcaa
      541 gctccctgat ggtgccggcg ctgctgcgcg gactgctcgc ctgaaccgaa aaccacaggt
      601 cgacgatgag cgcgtgtgcc cgccagcgcc gatgaaactc gtcgagcatt tcactgcgaa
      661 aagaatcgct gagaccatcg acgtcggcga cggcccggag ggcggcgctg cggtccgtga
      721 ggttgtcggc cgaccggtaa tgatcctcga gcaacgactc cagagcggac ccggtctcga
      781 cccgggcaag atacccgagg gccgtgttgc gcagggcccg gcgggacatg ccgtcgggct
      841 cgggcgcgta ggcacccgag gccgcgttgg cctcgtaaag cgcgcgccac cgctcggcgt
      901 gattggccgc caccgacacc acgagccggt ctcgggcatc gcaaacggct tctacctcga
      961 agtcgtcgag cgcctggaag agataaccct cgtccgcgac gtgcagtaac tccgacagca
     1021 gaaagcgctg ctcggcgctg gccccgccgg ccagcgcagg atcgatgaag ttgcccagca
     1081 gcgctacgag ctcggcggcg ggttcgccgc cggagcgcat ggcgtcgatc tccctgacca
     1141 ccagcgtctg cagcgcatcc cagcgcgcaa acccatcgga gtcgtgaaga gccagaaagg
     1201 caagcgtctc ggggtcacgg ggatattcca ctcgaaccgg agcggagaac ccccgcagga
     1261 aactcaccgc cggacgcccg ggcagaccgg ggatgcgaat ctcggtgatc tcatccctta
     1321 cgtgaacgag cagcgaccgg tcgccctcgc gcacatcgac ctgggcgtcg ctcgagcagt
     1381 ccaggccccc atcgaccagg tcgcggccgg caccatcgag cagcccgatc agaacgggaa
     1441 ggtggaaggg cttcttgctg ccctgcccgg gcgtcggcgg gcaggactgc cggatgcgca
     1501 ggctcagctc gccatcgtgc cagccctcct ccacctgcaa tcgtggcgtc ccggcctgct
     1561 cgtaccagcg ccggaactgt ttcagatcga gggcggtcac gtccgccatg gcgcccagaa
     1621 agtcgtcggt cgttaccgca cagccgtcgt agcgatcgaa gaagaggtcc gaaccacggc
     1681 ggaaccgttc ctctccgagg agggtccgca gcattcggac cacctcgctg cccttctcgt
     1741 agaccgtcgt ggtgtagaag ttggagatct cgatgtaaga gtcgggccgc accgggtgcg
     1801 ccatggggcc ggcgtcctcg ggaaactgcg acgaacgcag aaaattcacg tcctcgatgc
     1861 gcttgaccgc ccggaagttc atgtcggagg aaaactcctc gtcccggaaa acggtgaatc
     1921 cttccttcaa gctcagctga aaccagtcgc gacaggtgac gcggttgccg gaccagttgt
     1981 gaaaatattc gtgagccacg acgctctcga cacgcagatg ggcggcatcc gtggcggtgt
     2041 ccggcgaagc cagaacgcat ttggtgttga agatgttcag gcccttgttc tccatggcgc
     2101 ccatgttgaa atcttccacc gccacgatca tgaggatgtc gagatcgtac tccctgccga
     2161 acgtctcctc gtcccagcgc atggcgcgct tgagcgcttc catggcgtaa ccgcactggc
     2221 cgatgttctg tggctccgag aagatccgaa gcgccacgcg acgtccggaa gcggtcacga
     2281 actcgtcttc catgaccgcg aggtcgccgg ccacgagcgc aaagagatag ctcggcttgg
     2341 gaaacggatc ctcccaggtc acccggcggc gcccttcgcc cgccgggacg tcggagatgg
     2401 gatttccgtt ggcgagcagg accgggtaga gcgatgcgtc tgcttccagg gtcgtggtga
     2461 agcgcgcgag aacgtcggga cgatcctgat agtaagtgat ccggcggaac ccctcggctt
     2521 cgcattgggt gcagtacatg ctccctgatt tgtatagacc ttccaacgcc gtgttctttt
     2581 ccggctcgat acgggtcacg atcgtgatct cgcagcgctc cggcaggtcg aagagggtca
     2641 gcgactccgc atcgaccacg aactcgttgc cacccagcaa ccggccatcc agggccaccg
     2701 agagcagctc caggttctga ccatccagcc gtagcaccgt gtccgtgccg cctgcgcggc
     2761 gctccacgct cagctggctc tcgacgcgcg tgtagccatc gcgtacgtcg aagctcagcc
     2821 gggtatccag ggtccggtaa gacggcggct cgtagtcccg gaggaacacg ggttgaggga
     2881 cgttgctact catcggcaaa gctccatatg caaccgttga cggcctcgcg ccgatcagcg
     2941 accggagccg accagacgac ccgtgacgat gccgagaact gctccggcgg cgagtccgaa
     3001 ccaatgggcc gcgttggcca cgtgaagacc gaaatactcg gtcacgcccg tgctgaacag
     3061 caccaggaag aacagcagcc cacccgcgac agcggttggc acctgccatt gaggcacggc
     3121 gggcgccaga cggccggccg ccaggacaaa gcccaacagg ccgaaggcga cgccggaaag
     3181 cccgccgaac aggggattcg cgttcatcgc gtactgcccg agattggcca cgagcgaaac
     3241 cacgaggacg attgcccaga accagagtgc cccaagccga ctttcgatcc gccggcccat
     3301 ctcgatgacg acagcgcagt tgaacagaag atgcacggcg ttgaagtgga tcagcatggg
     3361 cgcgaaccag cgccagaccg cggcctccgc caccagctcg accagcgtcc cgggagcgga
     3421 tccaagccga atcagatcca cgatcgtcag ccagctggca acatacgtta cgcggccttc
     3481 ggccagcggc atggtaaacg gaaacagcgc cacggccagg gcgaccagga ccgcaagccc
     3541 cgggtacctg cggatctgcg cgagcagccc cggacggcgc tgcggcagag gctgtgcctc
     3601 gacgaccaga gaacccgaag ccagcgcctc gtagacgtcc cgaaccaatg cggcctgggc
     3661 accgtccgcc agctcgacga tctggcgccc gcgttcctcg tagatgcgat gacgcaccct
     3721 gcgttcccag agcaggcggg acagcaccga gagatcttcg tccagatccg tctcgatcac
     3781 gcgcatgaag cacagccaca gtggtagtaa catgcaagcc taccaaatcg gcgcaacgga
     3841 cgagatgcac gcagtagacg aggaagttct caatcaggtg accgagtggc tgctggccga
     3901 tcgcccctgc tggctggcga ccgtcgtggc cacctgggga tcctctcccc gccccgtcgg
     3961 gtcgttgctc gcgtgcaatg ccgaagggca aatcgtggga tcgctgtcgg gcggttgcgt
     4021 cgaggacgac cttctcgaga agctcacgag cggggagctc gctgccgagc acgc
//
LOCUS       DBNHOX010000492         8944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1259772, whole genome shotgun sequence.
ACCESSION   DBNHOX010000492 DBNHOX010000000
VERSION     DBNHOX010000492.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1259772"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="AC16UZ_10775"
     CDS             complement(<1..627)
                     /locus_tag="AC16UZ_10775"
                     /inference="COORDINATES: protein motif:HMM:NF023326.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HIR6247209.1"
                     /translation="MREWHALHRQLLLACRELALKPNDQPADYSAVHRALLQGSLSLV
                     GLHEEKGSYLGPRNLRFRIFPGSALARRGPKWLMAGEITETRRVYARCVAGVEARWIE
                     EAAGDLVKRRYSEPRWSVRRGEVIALETVTLYGLPLADGRRVSYRKVDPDACRELFIT
                     EALVRGGLEGPFEFLLHNRGLIREIVDEESKGRRRDLLIGDAAQADLYA"
     gene            complement(784..2334)
                     /gene="hrpA"
                     /locus_tag="AC16UZ_10780"
     CDS             complement(784..2334)
                     /gene="hrpA"
                     /locus_tag="AC16UZ_10780"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HIR6247210.1"
                     /translation="MASRRADTSFDPAAVMRRDVHRLKSLRRSNPSAWQALREQSEAR
                     LLARRGQRLEIAYPPELPISQHADEITRLLADHQVIVVAGETGSGKTTQLPKMCLEAG
                     FGYRGMIGHTQPRRLAARAVAARVAEELGARVGEAVGYAVRFSDQVGEHTLVKLVTDG
                     LLLNEIRTDRFLDAYEVIIVDEAHERSLNVDFLLGYLRRLVDRRHDLRVIITSATIDV
                     ASFSEHFGGAPVVEVGGRGFPVETRYADEEALADRSAEELIVDRIRSIEKTPVAGSAR
                     DILVFQSGEREIFDTAHALRQALDERYEILPLYARLSARDQQRIFKPSGYRRVVIATN
                     VAETSLTVPNIGFVIDPGFARVSRYSFRSKLQRLPVEPISQASADQRLGRCGRVAPGV
                     CYRLYTENDYLSRPPYTDPEIKRTNLAQVVLQMRSLGLGKVAGFPFLDPPDPRAVRDA
                     ERLLHELGALEGGGLSKTGRMMARLPVDPRLARMIIAGHEHRSLSEVLVIVSALAAQD
                     PRERPLER"
     gene            complement(2354..3526)
                     /locus_tag="AC16UZ_10785"
     CDS             complement(2354..3526)
                     /locus_tag="AC16UZ_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012722828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6247211.1"
                     /translation="MLINFFLTLRKYKVPVSIRELMDLLEALSLRLVYANIDDFYLLS
                     RTCMVKDEKHYDKFDRAFAAYFKGLEDLHGMLESLIPDEWLRREFEKSLTAEELEKIQ
                     SLGGLEKLIEAFQEARAEEERRQAEQARQGEEGEEGENPDRQGQKGPGGLGKGKKKKA
                     KKVWDQRQYKNLDDSVELGTRNIKMALRRLRKFARSGKEEELDIDTTIRSTAHNGGML
                     DIRMRPERKNTVKVLLFLDIGGSMDSHVKVCEELFSATRTEFKHMESFYFHNFIYESV
                     WKDNRRRMNERLSTWEILNKYGQDYKVVFVGDATMAPYEITHAGGSVEHWNEEPGAEW
                     MNRFTEIYDRLVWINPTPQETWEYSSSVGMTQQLVNGQMYPLTIRGLEEGMSFLSK"
     gene            complement(3568..4416)
                     /locus_tag="AC16UZ_10790"
     CDS             complement(3568..4416)
                     /locus_tag="AC16UZ_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6247212.1"
                     /translation="MIFDSTDTYISTDELSMAVDAAVKLERPLLVKGEPGTGKTMLAE
                     EIAKSLGLRLIQWHIKSSTKAINGLYEYDAVSRLRDSQLGDERVQDIRNYIKKGKLWE
                     AFEADERVVLLIDEIDKADIEFPNDLLQELDRMEFFVYELGETIKAKQRPVVVITSNN
                     EKELPDAFLRRCFFHYINFPDRETMQQIVEVHHPSIKSELVKEAMEIFFDVRKVPGLK
                     KKPSTSELIDWLKLLMADDIPDEILTNRDTSKAIPPLYGALVKNEQDVHLLERLAFMN
                     RRDSRG"
     gene            complement(4667..5029)
                     /locus_tag="AC16UZ_10795"
     CDS             complement(4667..5029)
                     /locus_tag="AC16UZ_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247213.1"
                     /translation="MGNAWVWAAIVVGGLGAAAGLHMALSGLLAREIRLAVVFVLLCL
                     LITPAPVPEYAGSYAPALIILAFEGLFQRGGDPFTALKILLAVAALASGAAVLMVLGW
                     RRWASTSLPNAESADSSL"
     gene            complement(5029..5469)
                     /locus_tag="AC16UZ_10800"
     CDS             complement(5029..5469)
                     /locus_tag="AC16UZ_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HIR6247214.1"
                     /translation="MDRFWEHKTLGELSAEEWESLCDGCARCCLVKLEEEGSGCVHYT
                     GLVCDLLDLETCRCTRYPDRHRLVKDCIDFSAEVMASLSWLPRSCAYRRVAEGRGLAD
                     WHPLVSGSPDSVHRAGISVLGRVIPAGSVHEDEFEEHLIQWVET"
     gene            complement(5469..5720)
                     /locus_tag="AC16UZ_10805"
     CDS             complement(5469..5720)
                     /locus_tag="AC16UZ_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017526690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HIR6247215.1"
                     /translation="MSRLVQIYRSSRRNGMYLYVDAGEDLARVPEALMKSFGRPVAAM
                     SLALTRDRQLARADAAAVLKNIEECGFYLQMPPSVDELS"
     gene            complement(5717..6829)
                     /locus_tag="AC16UZ_10810"
     CDS             complement(5717..6829)
                     /locus_tag="AC16UZ_10810"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:NF013755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HIR6247216.1"
                     /translation="MPCRADAASVSWIDDTGALVDAVASWGDVIGLDTEFQRTDTFFP
                     VPGLYQVISGEDVYFLDPLSIDDFSAFADVLHDPAIVKVMHACSEDLELIRHHLGVEP
                     QGIFDTQLAYAFQNSRYSVSYANLVAALLGHDLGKHATRSNWLRRPLTEEQLHYAWED
                     VRYLPEVYEALTARLDELGRRSWFADAMAEKGRVQGAHPDEYYRGVKKAWRLDALELG
                     VLKRLCAWRERRAMAEDVPRNRVVWDEHLVEFARLGSLSETDVHERLPGPVARRYASE
                     LTAEHREGVSAGLAEPLLARPLSPSESKVSRTLRDLAWHKADTMEFARELLARKRDVE
                     ACIRHWLDTGELSPAYSGWRAGLIGEEFLAVLEHAP"
     gene            complement(6798..7397)
                     /gene="recR"
                     /locus_tag="AC16UZ_10815"
     CDS             complement(6798..7397)
                     /gene="recR"
                     /locus_tag="AC16UZ_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR6247217.1"
                     /translation="MGISPLIDRLIGAFQVLPGVGPKGAQRMVLHLLQRDREGARQLA
                     ETLLESLDRVSRCSRCRNLSEEAVCSICRDPRRDDDVLCVVESPADILAIEAAGGFRG
                     RYFVLHGRLSPIDGVGPEELGVDTLQRRVAELEPKEVILATNPTVEGEATAHYISKLL
                     KADVPNISRIAHGVPVGGELEYIDGGTIVHALQGRRSIS"
     gene            complement(7397..7714)
                     /locus_tag="AC16UZ_10820"
     CDS             complement(7397..7714)
                     /locus_tag="AC16UZ_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR6247218.1"
                     /translation="MKGLGDFMKQAQAMQERMQQVQEEIAKLEVVGESGAGLVKVTMN
                     GRHEVRRVHLDDSLLAEAKDVLEDLVAAACNDAVRKVEAAQRDKMAGMASGLGLPLDK
                     FPF"
     gene            complement(7711..>8944)
                     /locus_tag="AC16UZ_10825"
     CDS             complement(7711..>8944)
                     /locus_tag="AC16UZ_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HIR6247219.1"
                     /translation="RELLENAQYLPTRGRYKVYLIDEVHMLSTPAFNALLKTLEEPPE
                     HVKFLLATTDPKKVPVTVLSRCLQFQLKNISSERIAAYLAEVLQAEEISFEPEALETI
                     AAAALGSMRDALSITDQAIAYGGGSLGHADVVEMLGLVGRDEVHSLLEALASGDAERV
                     LAMTAELAERGADFEEVLSGVIAAFHRMAVEQATGSGANALFGPEVVQLYYQIALMGY
                     RDMHIVPDPRSGFEMTLLRMIAFAPDEGLLTQSTAPSTTAERGAKERPVPESAGGRDG
                     VESTLADGTPWHEVVESLEIGGVARMIAEHSVVGSHQGDRLRLTLDTAHDTLLNSAQQ
                     AALQRALGGKLGSDLLLEIEAGSCAAETPAQRNARIAAERQHQAEQTLKRDGVVQSLL
                     SEFDGTLGDVKPMEDSAG"
BASE COUNT         1645 a         2966 c         2681 g         1652 t
ORIGIN      
        1 cgcgtaaaga tccgcctgag ccgcatcgcc gatcagcaaa tcgcgccggc ggcccttgga
       61 ttcctcatcg acgatctccc gaatcaggcc ccgattgtgg agaaggaact cgaagggccc
      121 ttcgagccct ccgcgcacga gcgcctcggt aatgaagagc tcacgacagg catccgggtc
      181 taccttccga tagctcacgc gacgcccgtc cgccaaagga aggccgtaca gcgtgacggt
      241 ctcaagcgcg atcacctcgc ctcttcgtac gctccagcgc ggctcggagt agcggcgctt
      301 gaccagatcg ccggcagctt cctcgatcca tcgagcctcg acgccggcca cgcagcgcgc
      361 gtaaacccgg cgtgtctcgg tgatctctcc ggccatcagc cacttcggcc cacgcctggc
      421 cagcgccgat ccggggaaga tacggaatcg caggttacgc ggccccagat agctgccctt
      481 ctcttcgtgc agacctacca ggctcaagga tccctggagc agcgcgcgat gcacggccga
      541 gtaatcggcg ggctgatcgt tcggtttgag cgcaagctca cggcaagcca gcagcagctg
      601 ccgatgcagc gcatgccact cccgcatccg cgtggcggaa atgaagcgcg agccgagctc
      661 gcgagacagc ctggcgcgag aaagacgctg ccgggcttcc aggtaccact gccagatgtt
      721 cagaaaggcg agaaaatccg agcgcggatc cgagaactcc gaatgggccc ggtcggcgga
      781 cccctagcgt tccagcggcc gctcacgggg atcctgggcg gccagagcgc tcacgatcac
      841 cagaacctcg gacagtgacc ggtgctcatg gcccgcaatg atcatccggg ccaggcgcgg
      901 gtccaccggg agccgggcca tcatgcgccc ggtcttggaa agaccgcccc cttcaagcgc
      961 gcccagctcg tggagcagac gctccgcgtc acggactgcc cgggggtcgg gcgggtcgag
     1021 aaacggaaag cccgcgacct tacccaaccc caacgagcgc atctgaagca cgacctgggc
     1081 gaggttcgtg cgcttgattt ccggatcggt atatggaggc cggctcagat agtcattctc
     1141 ggtataaagc cgatagcaca cgcccggcgc cacccggccg cagcggccca gacgctgatc
     1201 cgcgctggcc tgtgatatgg gctcgacagg caaccgttgc agcttggagc gaaacgagta
     1261 gcggctcacc cgggcaaagc cgggatcgat gacgaagccg atgttgggca ccgtcaacga
     1321 cgtttcggcc acattggtgg cgatcaccac ccgtcgatat cccgagggct tgaagatgcg
     1381 ctgctggtcc cgggccgaca ggcgcgcgta cagaggcagt atctcgtaac gctcatcgag
     1441 cgcttggcgc agcgcgtggg cagtatcgaa tatctcccgc tcgccggact ggaacacgag
     1501 gatgtcccgc gctgatcccg ccacgggcgt cttctcgatg gaccggatac ggtcgacgat
     1561 cagctcctcg gcgcttcgat ccgccagcgc ttcctcgtct gcataacggg tctcgacagg
     1621 aaaccctcgt ccgccgacct ccaccaccgg tgccccgccg aaatgctcag agaaagaggc
     1681 cacgtcgata gtggcgctgg tgatgatgac cctcagatcg tggcggcggt cgaccagcct
     1741 gcggaggtag ccgagcagaa agtccacgtt caggctgcgc tcgtgggcct cgtcgacgat
     1801 gatcacctcg taggcgtcga gaaacctgtc cgtacgtatc tcgttgagaa gcagaccgtc
     1861 cgtcaccagc ttcaccagcg tgtgctcgcc cacctgatcg ctgaaccgga cagcgtagcc
     1921 caccgcctca ccgacccggg cgccgagttc ctccgcgacc cgggcggcca ccgctcgagc
     1981 cgccagacgc cttggctgcg tgtgcccgat catcccgcga tagccgaagc ccgcctcgag
     2041 gcacatcttg ggcagctgag tggtctttcc cgacccggtc tccccggcca cgacgatgac
     2101 ctgatgatcg gccagcagcc tcgtgatctc gtcggcatgc tgactgatgg gaagctcggg
     2161 cgggtaagcg atctccagcc gctggccgcg ccgggcgagg agcctggcct cggattgctc
     2221 acgaagcgcc tgccaggcgg aaggattact ccttctcagt gatttgagac gatggacgtc
     2281 ccgccgcatg acggcagcgg gatcaaacga ggtatcggcg cgtcgagaag ccatcgtgag
     2341 atggtcggcc aggctatttg gaaaggaagc tcattccctc ttcgaggccc cgaatcgtca
     2401 gggggtacat ctgcccgttc acaagctgct gggtcatgcc gaccgacgag gaatactccc
     2461 aggtttcttg aggagtggga ttgatccaga cgagccggtc gtaaatttcg gtgaagcggt
     2521 tcatccactc cgcgccgggc tcttcgttcc agtgctccac gctaccaccg gcatgggtaa
     2581 tctcgtaggg agccatcgtg gcgtctccca cgaagaccac tttgtagtct tggccgtact
     2641 tgttgaggat ctcccacgtc gacagacgct cgttcatccg ccggcggttg tctttccaca
     2701 ccgactcgta gatgaagttg tggaagtaga agctttccat gtgcttgaac tcggtccggg
     2761 tcgccgagaa aagctcctca cagaccttca catgagagtc catggaaccg ccgatgtcga
     2821 ggaaaagaag caccttcacc gtattcttgc gctcgggccg catgcgtatg tcgagcattc
     2881 cgccgttgtg cgccgtcgac cggatcgtgg tgtcgatatc cagctcttct tccttcccgg
     2941 aacgggcaaa cttcctcagc cggcgcagcg ccatcttgat gttgcgggtg ccgagctcca
     3001 ccgagtcgtc gagattcttg tactgccgct gatcccagac tttctttgct ttcttctttt
     3061 tgcctttgcc aaggccgccc gggcccttct ggccctgccg gtcgggattc tcgccctcct
     3121 cgccttcctc gccctgacgc gcctgctctg cctgacgccg ttcctcctcg gccctggctt
     3181 cctgaaacgc ctcgatgagc ttctccaggc cgccgaggga ttgaatcttt tcgagctctt
     3241 cggctgtcaa cgatttttcg aattcgcgac gcagccactc gtccggtatg agggactcca
     3301 gcatcccgtg caggtcctcc agccctttga agtaggctgc aaaggcccgg tcgaacttgt
     3361 cgtagtgctt ctcgtccttg accatgcagg tgcggctgag cagatagaaa tcgtcgatgt
     3421 tggcgtaaac cagccgaagg ctgagcgcct ccagcagatc catcaactcg cggatggaga
     3481 ccggcacctt gtatttgcgc agggtgagaa agaagttgat gagcattgtc cggtcgtgct
     3541 ccctcggtgg ctatcgaccc gccgcttcta gccgcggctg tcccgacgat tcatgaacgc
     3601 cagtctctcg agcaggtgca cgtcctgttc attcttcacc agggcgccgt aaagcggcgg
     3661 tatggccttg ctcgtatccc gattggtgag aatctcgtcc ggaatgtcgt ccgccatcag
     3721 cagcttcagc cagtcgatga gttcggatgt ggacggcttt ttcttcagcc ccggcacctt
     3781 gcgcacatca aagaagatct ccatcgcttc cttgaccagc tcggacttga tgctcggatg
     3841 atgcacctcg acgatctgct gcatggtctc ccgatccggg aaattgatgt agtggaagaa
     3901 gcagcgacgc aagaaggcat cgggcagctc cttttcgttg ttcgacgtga tgacgacaac
     3961 cggccgctgc ttcgccttga tggtttcgcc gagctcgtag acgaaaaact ccatccgatc
     4021 gagttcctga agcaggtcgt tcggaaactc gatgtccgcc ttgtcgatct cgtcgatcag
     4081 cagcaccacg cgctcgtcgg cctcgaaagc ctcccagagc ttgcccttct tgatgtagtt
     4141 gcgaatgtcc tgaacccgct cgtcgcccag ctgggaatcg cgcagccgcg acaccgcgtc
     4201 gtactcgtag agcccgttga tcgccttggt gctggacttg atgtgccact gaatcagtct
     4261 gagcccaagc gacttggcga tttcctcggc cagcatggtt ttgccggttc ccggctcgcc
     4321 cttgacgagc agcgggcgct cgagcttgac cgcggcgtcc acggccatgc tcagctcatc
     4381 ggtcgagata taggtgtcgg tactgtcgaa gatcattcgg ctctactcgt gaattgatgt
     4441 ttcgcgcgag tgaaggaccc cccagaaaac tgcgtcagca ggcgtcgcgc gtacgaagtc
     4501 cgctacttta atcgacgctc cgaggcctag caaggcgcgg cccagcctga gccccgtgcg
     4561 accgggagca tgatcctgtc cgcaagctca ttgccggacg accaacgcaa ccgggagtta
     4621 gatggcaatg ataaatatat ataaaacaat aagttggata tcttttctag agactactgt
     4681 cagcgctttc cgcgttcggg agggacgtgc tcgcccagcg ccgccagccg agcaccatca
     4741 acacggcggc gcccgaggcc aacgctgcca cagccaggag gatcttgagc gcggtgaaag
     4801 gatcgccgcc ccgctgaaag agcccttcga aggcgaggat gatcagagcc ggcgcgtagc
     4861 ttccggcata ctctggaacc ggcgccggcg tgatgagcag gcacagcaac acgaaaacga
     4921 ccgccagcct gatctcgcgg gcgagcaacc cgctcagcgc catatgcagc cccgctgcgg
     4981 cacccaggcc gcccaccacg atagccgccc atacccatgc gttccccatc aggtctcaac
     5041 ccactggatc agatgttctt cgaactcgtc ctcatgcacc gagcccgccg ggatcactcg
     5101 accgagcacc gagatgcccg ctcgatgcac ggaatccggg gagccggaaa cgagagggtg
     5161 ccagtcggcg agcccccgac cttcggcgac acggcggtag gcacagctgc gcggcagcca
     5221 ggagagcgac gccatcacct ccgcggagaa gtcgatacag tccttgacga ggcggtgtct
     5281 gtccggataa cgggtgcagc gacaggtctc gaggtccagc agatcgcaaa ccagccccgt
     5341 gtagtggacg catcccgaac cctcctcttc gagcttgacg aggcaacatc gggcgcagcc
     5401 atcgcagagc gactcccact cctcggccga cagctcacca agcgtcttgt gctcccagaa
     5461 ccggtccatc aggaaagctc gtcgaccgat ggcggcatct ggaggtagaa gccgcactct
     5521 tcgatattct tgagcacggc cgctgcatcc gccctggcca gctgccggtc cctggtcagc
     5581 gcgagggaca tggccgccac cggtcgcccg aagcttttca tgagcgcttc cggtacacgg
     5641 gcgaggtcct cgccggcgtc cacgtagaga tacatgccat tccggcgcga gctgcgatag
     5701 atctgcacca ggcggctcaa ggcgcgtgct ccagaaccgc caggaactcc tcaccgatca
     5761 agccggcccg ccagccggag taggcgggcg acagctcgcc ggtgtccagc cagtggcgaa
     5821 tgcaggcttc cacgtcacgc ttgcgggcga gcagttccct ggcaaactcc atcgtatcgg
     5881 ccttgtgcca ggcaaggtcg cgcagggtcc ggctgacctt gctctccgac gggctcaagg
     5941 gtcgagcgag cagcggctcg gcgagcccgg ccgagacgcc ttcccgatgc tcggctgtga
     6001 gctcggaggc gtaccggcgg gcaacagggc ccggcagacg ctcgtgcacg tccgtctcag
     6061 aaagcgatcc gaggcgcgcg aattccacca gatgctcgtc ccagaccacc cggttgcgag
     6121 gcacgtcttc ggccattgcc cggcgctcgc gccacgcgca aagccgcttg agcacaccga
     6181 gctcaagagc gtccaggcgc caggccttct tgacgccacg gtagtattca tccggatggg
     6241 ctccctgaac ccgacctttc tcggccatgg cgtcagcgaa ccacgagcgt ctgccgagtt
     6301 cgtcgagccg cgcggtcagc gcttcgtaaa cttcgggaag gtagcgcaca tcctcccagg
     6361 catagtgcag ctgctcctcg gtcaggggcc ggcgcaacca gttggaccgg gtggcgtgct
     6421 tgcccagatc gtgtcccagc aatgccgcta ccaggttggc gtagctcacg ctgtacctcg
     6481 agttctgaaa cgcataggcc agctgggtat cgaatatccc ctggggttcc accccgaggt
     6541 gatgacgaat gagctcgagg tcctcggagc aggcgtgcat caccttgacg atggccggat
     6601 cgtgcagcac gtcggcaaac gccgagaaat cgtcgatgga cagcgggtcg aggaaataca
     6661 cgtcctcgcc gctgatgacc tgataaaggc cgggcaccgg aaaaaaggtg tccgtgcgct
     6721 gaaactccgt gtccagaccg atcacgtcgc cccaggacgc caccgcgtcg accagagcgc
     6781 cagtgtcgtc gatccagcta actgatgctg cgtcggccct gcaaggcatg aacgatcgtt
     6841 cctccgtcaa tgtattcgag ctccccgcca acgggcaccc cgtgggcgat tcgcgatatg
     6901 ttgggtacgt cggccttcag cagcttgctg atgtaatgcg ccgtggcttc gccctccacc
     6961 gtggggttcg tggcgagtat gacttccttc ggttccagct ccgcgaccct gcgctgcaac
     7021 gtgtctacgc cgagttcctc gggccccacc ccatcgatgg gggacagccg tccgtgcagg
     7081 acgaagtagc gtccgcgaaa gccgcccgcc gcctcgatgg cgagaatgtc cgcgggcgac
     7141 tcgaccacgc agagaacgtc atcgtcgcgg cgcggatctc tacagatcga gcagacggcc
     7201 tcttccgaca ggttccggca gcgtgaacag cgcgacacgc ggtccaggga ctcaagaagc
     7261 gtctccgcaa gttgccgggc gccctcacga tcgcgctgca acaggtgcag gaccatgcgt
     7321 tgcgctccct tggggccgac tccgggaagc acctgaaagg ccccgatcag ccggtcgatt
     7381 agcggactga ttcccatcag aaagggaact tgtcgagcgg caggccgaga ccggatgcca
     7441 ttcccgccat tttgtcccgt tgggccgctt ccaccttccg aaccgcgtcg ttgcaggcgg
     7501 cagcgaccag gtcctcgagc acgtctttcg cttccgcaag caggctgtcg tcgagatgga
     7561 cgcgccgaac ttcgtgccgc ccgttcatcg tgaccttgac gagaccggcc ccgctctcgc
     7621 ccacgacttc gagcttcgcg atttcctcct gaacctgctg catgcgttcc tgcatggcct
     7681 gggcctgttt catgaagtca ccgagccctt tcatcctgcc gaatcctcca tgggtttgac
     7741 gtctcccagg gtgccgtcga actcgctgag caacgactgc acgaccccgt ctcgcttgag
     7801 ggtctgctcg gcctgatgct gacgctccgc cgcaatgcgc gcgtttcgct gcgccggtgt
     7861 ctccgccgcg cagcttcccg cttcgatctc gagcagcaga tctgatccca acttgcctcc
     7921 gagggcgcgc tgaagcgccg cctgctgggc ggagttgagc aacgtgtcat gggccgtatc
     7981 gagcgtaagc cgcagccggt ccccttggtg ggaacccacc acgctgtgct ctgcaatcat
     8041 ccgtgcgacg ccgccaattt caagggactc gaccacctcg tgccacggcg tgccgtcggc
     8101 cagggtcgac tcgactccgt cgcgcccccc ggcgctttcc ggcaccggcc gctcctttgc
     8161 tccccgttcg gccgtcgtcg agggcgcagt gctttgagtc agaaggccct cgtcgggagc
     8221 aaacgcaatc atacgcagca gggtcatctc gaaaccgcta cgcggatccg gaacgatgtg
     8281 catgtcccga tagcccatga gcgcaatctg gtagtagagc tgaaccacct ccggcccaaa
     8341 gagcgcgttg gctcctgaac ccgtcgcctg ctccacggcc atacggtgaa aggccgcgat
     8401 cacgccgctt agaacttcct caaaatccgc ccctcgctct gccagctccg ccgtcatggc
     8461 aagcacccgc tcggcgtcgc cactggccag tgcttccaac aggctgtgca cctcgtcgcg
     8521 tcccaccagg ccaagcattt ctaccacgtc ggcgtgcccg agagacccgc cgccgtaagc
     8581 gatggcctga tcggtgatgg aaagcgcgtc ccgcatgctg cccagcgcgg ccgcggcgat
     8641 ggtctcaagt gcctcgggct cgaaggagat ctcctctgcc tggagcacct cggcaaggta
     8701 ggcggcgatt cgctcgctgg agatgttttt cagctgaaac tgcaggcagc gggagaggac
     8761 ggtgaccggt acctttttcg ggtccgtcgt cgccagcaga aacttcacgt gctccggcgg
     8821 ctcctcgagc gtcttcagga gggcgttgaa agccggcgtc gagagcatgt gaacctcgtc
     8881 gatcaggtag accttgtacc ggccgcgtgt cggcaggtac tgggcgttct ccaagagctc
     8941 gcgg
//
LOCUS       DBNHOX010000493         4732 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_104226, whole genome shotgun sequence.
ACCESSION   DBNHOX010000493 DBNHOX010000000
VERSION     DBNHOX010000493.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_104226"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..399)
                     /locus_tag="AC16UZ_10830"
     CDS             complement(34..399)
                     /locus_tag="AC16UZ_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010795388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6247220.1"
                     /translation="MARILVVDDSPSETHQLANALSRHGHQVLTAASGSDGISLANEE
                     QPDIILMDVVMPGINGFQATRQLTRSQGTSHIPVIIVSSKSQDADRVWGKRQGAQGYL
                     TKPVDDRTLISTVNEALSA"
     gene            complement(425..805)
                     /gene="pilG"
                     /locus_tag="AC16UZ_10835"
     CDS             complement(425..805)
                     /gene="pilG"
                     /locus_tag="AC16UZ_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HIR6247221.1"
                     /translation="MEQELKGVKVLVIDDSKTIRRSAENLLTKEGYDVITATDGFDAL
                     AKIVDSRPGIIFVDVMMPRLDGYQTCALIKNNSEFKTTPVVMLSSKDGLFDKAKGRIV
                     GSDQYITKPFSKDELLGTIRNLVH"
     gene            1061..2047
                     /gene="gshB"
                     /locus_tag="AC16UZ_10840"
     CDS             1061..2047
                     /gene="gshB"
                     /locus_tag="AC16UZ_10840"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HIR6247222.1"
                     /translation="MSLKIAFVMDPLPTLSLKKDSTLAMIRAAQLKGWQVGYLEQGDL
                     LLHEGAPCGMLRSLELVGPFADSLDPAEAGDRWYQLSEAALTPLAELDVIMMRKDPPF
                     DMEYIYSTYLLERAEALGVLVVNKPQGLRDCNEKFFATAFQECCPPLVVTRSEAALRE
                     FQRTHGNVVFKPLDGMGGQGVFRIMEGDENLGVIIETLTKGGTAQIMGQKFIPEIASG
                     DKRVLLIDGEPIPYALARIPMAGEARGNLAAGGTGEGRELTERDRWIAAQVGPELRRR
                     GLLFVGMDVIGDYLTEVNVTCPTCIRELDAQFSLNIGGMLMDTISAKLDAQA"
     gene            2072..2932
                     /locus_tag="AC16UZ_10845"
     CDS             2072..2932
                     /locus_tag="AC16UZ_10845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6247223.1"
                     /translation="MSAAAITPRDRLGFTLFLAVSLHAAIILGVSFTADLDLLESPTV
                     EVTFAQHSDLEAPEDADFIAQTNQLGSGTESEVLETTTDQEAEFFANEFADAISNPLP
                     TIEQNQLDSRELVTTISEVDDVAPIEEETPVEENVNPQPATDIRFDDLAKEIASLEAR
                     IAADQQAKAKQPRVKRLTSVSAKSADEAAYLNMWRQKVERIGNANYPDSRVYGNLRML
                     VVLSYDGTLKDVRILESSGHKSLDDAALRIVRLAAPYQQFPVEMRKKYDQLEIIRTWK
                     FSRSGARLGS"
     gene            2922..3482
                     /locus_tag="AC16UZ_10850"
     CDS             2922..3482
                     /locus_tag="AC16UZ_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003297209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR6247224.1"
                     /translation="MAADTSLKNQFLLAMPGLAGTYFGDTVTYVCEHSADGAMGLMVN
                     RPTEVTLQALLADLGIDSSAVSADIPVMEGGPVASERGFVLHSDDRAFDSSLSLGDGL
                     MLNSTREVLEAIAAGEGPADFLVAMGYAGWGGGQLEQELLENAWLTCPGNTDVLFRTP
                     FAERVNQAAAALGIDFRLMSSQAGHA"
     gene            3490..3879
                     /gene="ruvX"
                     /locus_tag="AC16UZ_10855"
     CDS             3490..3879
                     /gene="ruvX"
                     /locus_tag="AC16UZ_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR6247225.1"
                     /translation="MKQGHVLAFDFGLKHIGVAVGQSVTRTASPLITIAARDGTPDWS
                     DVERLITEWQPAVLLVGLPLNMDDSESEMSLKARGFAERMGRRFGVPIELVDERLTSF
                     AARESGSDRPHEAAATMIAESWLAADG"
     gene            complement(3901..>4732)
                     /locus_tag="AC16UZ_10860"
     CDS             complement(3901..>4732)
                     /locus_tag="AC16UZ_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HIR6247226.1"
                     /translation="PLVSDLGLPPILEDLSMTRRGLIIFVGATGTGKSTSLAAMIGHR
                     NEHSSGHIISIEDPIEFVHQHRGCVVTQREVGVDTESFEVALKNTLRQAPDVIMIGEV
                     RTRETMEHAVAFAETGHLCLCTLHANNANQALDRIIHFFPSDRHSQVWMDLSLNLRAM
                     VAQQLVPTVDGQGRRAAIEILINTPLMADHIRKGEVHLLKELMSKSTEHGMQTFDQAL
                     YQLYSEGEISHEAALQHADSPNDLRLMIKLGSDANPDLMGQVEGLTIEEDEPAGTFLR
                     "
BASE COUNT          876 a         1469 c         1483 g          904 t
ORIGIN      
        1 gtatgttttg cgcttgaccg ttcatgacaa ggattaggcg ctcaaagcct cgttgaccgt
       61 gctgatcagc gtgcgatcgt ccacgggttt ggtcaggtag ccttgcgcgc cctggcgttt
      121 gccccagacc cggtccgcat cctggctctt ggaggagacg atgatcaccg gaatgtggct
      181 ggtaccctgg cttctcgtga gctgccgcgt tgcctgaaag ccgttgatac caggcatcac
      241 gacgtccatc agaatgatgt caggctgctc ttcgttggcc agactgatcc cgtcgctgcc
      301 ggaggcagcc gtcagcacct ggtgaccatg acgcgaaagc gcgttggcca gctggtgcgt
      361 ctccgaggga gagtcgtcga ctacgagaat ccttgccatc ttctgcgcgt cctgtgcctg
      421 cttcctaatg gacgaggttc cggatcgtgc cgagcagctc gtccttgctg aagggcttgg
      481 tgatgtactg atccgaaccc acgatccggc ctttggcttt gtcgaagagg ccgtccttgc
      541 tcgagagcat gactacgggg gtagtcttga attcgctgtt gttcttgatg agcgcgcagg
      601 tctggtaacc gtcaagacga ggcatcatga cgtcgacgaa gatgattccc ggccgtgaat
      661 cgacgatttt cgcgagcgcg tcaaagccgt ccgtggcggt gatgacgtca tacccttcct
      721 tggtcagcag attctcggcg cttcgacgaa tggtcttact gtcgtcgatt accagcacct
      781 taacgccttt gagttcctgt tccatatctg gtccttctac ttccgacttg accgtcgtgt
      841 caccgtccat tgagtatggc gtcatcaagg ctgtcggcag gattttcacg tacctaccga
      901 acgctcccgt tgtatcatga ttgtcccgaa acgatctata agcgttggat aagaaaggga
      961 attcgttctc atttttgggg ttacgctacg accgccaagc tagttaccgc cccagccgga
     1021 gcaacttctg tcccggctcg cccggagcac cacagaaccc atgagtctca aaatcgcctt
     1081 cgtgatggac cctctgccca ccctgtcgct gaagaaggac agcacgctgg caatgatccg
     1141 cgcagcccag ctcaaggggt ggcaggtagg ctatctcgag cagggggacc tgctgctcca
     1201 tgagggcgcg ccctgcggga tgctccgatc cctggagctc gtcgggccgt ttgccgattc
     1261 cctcgatccg gccgaggccg gcgatcgctg gtatcagctc agcgaagcgg cccttacccc
     1321 gctcgccgag ctggacgtga tcatgatgcg caaagacccg ccgttcgaca tggagtacat
     1381 ctactccacc tacctcctcg aacgggccga agcgttgggc gtgctggtgg tcaacaaacc
     1441 ccaagggctg cgcgactgca acgagaagtt ctttgccacc gccttccagg aatgttgccc
     1501 cccactggtc gtgacccggt ccgaagcggc gctgcgcgag tttcaacgga ctcacggcaa
     1561 cgtggtgttc aagccccttg acggcatggg gggccagggc gtgttccgca tcatggaggg
     1621 cgacgagaac ctgggcgtca tcatcgagac gctcacgaaa ggcggcaccg cccagatcat
     1681 gggccagaag ttcattccgg agattgccag cggggacaag cgcgttctgc tcatcgatgg
     1741 cgagccgatc ccctacgccc tggccagaat ccccatggcc ggcgaggcga gaggcaacct
     1801 ggcggcgggc ggcacgggcg agggacggga gctcaccgag cgcgaccgat ggatcgccgc
     1861 tcaggtcggt cccgagctgc gtcggcgggg cctgctgttc gtgggcatgg acgtcatcgg
     1921 cgactatctc acggaggtaa acgtcacctg cccgacctgc atacgggaac tcgatgcaca
     1981 gttttcgctg aatatcggcg gtatgctcat ggacacgata tctgccaagc tagatgctca
     2041 ggcctgaacg ccccgtcgac accctgcaaa catgagcgcc gccgccatca ctccacgaga
     2101 ccgtctgggc ttcaccctgt ttctggccgt ctcgctgcac gccgccatca ttctcggcgt
     2161 cagcttcacg gcagacctcg acctgctcga aagccctacg gtcgaggtga cgtttgccca
     2221 gcacagcgac ctcgaagcac ccgaggatgc cgacttcatc gctcagacca accagctcgg
     2281 cagcggcacc gagagcgaag tgctggagac cacgacggac caggaagcgg agtttttcgc
     2341 caacgagttc gccgacgcga tatccaaccc cttgccgacc atcgagcaga accagcttga
     2401 ctctcgggag ctggtgacga ccatctccga ggtagacgac gtggcgccca ttgaagaaga
     2461 gacgccggtg gaggaaaacg tcaatccgca gccggcaacc gacattcgct tcgacgatct
     2521 ggcgaaggag atcgcgagcc tggaagccag gatcgccgcg gaccagcagg ccaaggcgaa
     2581 gcagccaagg gtgaagcggc tcaccagcgt gtccgccaag tcagcagacg aagccgccta
     2641 cctgaacatg tggcgtcaga aggtcgagcg catcggcaac gccaactatc ccgacagccg
     2701 ggtgtacggc aatctgcgga tgctcgtggt gctgagctac gacggcacgc tcaaggacgt
     2761 ccggattctg gagtcttccg ggcacaaatc cctcgacgac gccgcgcttc gaatcgtccg
     2821 tctggctgcg ccctaccagc agtttccggt ggagatgcgc aagaagtacg atcagttgga
     2881 gatcattcgc acctggaaat tctcacgctc cggagcccgc cttggcagct gataccagcc
     2941 tgaaaaacca gttcctgctc gccatgcccg gcctggctgg cacctatttc ggggacacgg
     3001 tcacctacgt atgcgagcac agcgccgacg gcgccatggg cctgatggtc aatcgcccga
     3061 cggaagttac gctgcaggcg ctgctggccg atctcggcat agactccagc gccgtgtcgg
     3121 cggacatacc cgtgatggaa ggggggcccg tggcgagcga gcgcggattc gtgctgcaca
     3181 gcgacgaccg agcctttgac tcttcgctct ccctggggga cgggctgatg ctcaactcca
     3241 cccgcgaggt gctcgaggcc attgcggccg gcgaggggcc cgcggacttc ctggtcgcca
     3301 tgggatacgc cggctggggc gggggccagc tggagcagga gctgctggaa aacgcctggc
     3361 tcacctgccc tggaaacacc gacgtcctct ttcgcacgcc tttcgcagaa cgcgtgaacc
     3421 aggcggctgc cgcgctgggc atcgatttcc ggctgatgtc ctcacaggcg ggccacgcct
     3481 gacagccgcg tgaagcaggg tcacgtgctg gcgttcgact tcgggctgaa gcacatcggc
     3541 gtggccgttg gccaatcggt tacccgaacg gccagcccgc tgatcaccat cgcggccagg
     3601 gacggaacgc cggactggtc ggacgtggaa cggctgatta ccgagtggca gccggcggtg
     3661 ctgctggtgg gcctacccct taacatggac gacagcgaga gcgaaatgtc gctcaaggct
     3721 cgcgggttcg ccgagcgaat gggccgccgg ttcggcgttc ccatcgagct ggtcgacgag
     3781 cggctgactt cgtttgcggc gcgcgaatcc ggcagcgatc ggcctcacga ggccgcagca
     3841 acgatgatcg cggaatcctg gttggcggcc gatggctgaa ccgctgggga agagcggctc
     3901 tcagcgcagg aaggtacccg ccggctcgtc ttcttcgatg gtcaaccctt cgacctgacc
     3961 catcaggtcc gggtttgcat cggaacccag cttgatcatc agtctcaggt cgttcggcga
     4021 gtccgcatgc tgcagcgcag cctcgtggct gatctcgccc tcgctgtaga gctgatagag
     4081 cgcctggtcg aacgtctgca tgccgtgctc ggtggatttg gacatcagct ctttgagcag
     4141 atgcacctca cctttacgga tgtggtcggc catcagcggc gtgttgatca gtatctcgat
     4201 cgctgcccgc cgtccctgtc cgtccaccgt ggggacgagc tgctgggcca ccatggcgcg
     4261 caggttgaga gaaagatcca tccacacctg ggagtgacgg tcgctgggga agaagtgaat
     4321 gatcctgtcc agcgcctggt tggcgttgtt ggcgtggagg gtgcagaggc agaggtgacc
     4381 ggtttccgca aaggccaccg cgtgctccat ggtttcccgg gtccgcacct caccaatcat
     4441 gatgacgtcc ggcgcctggc gcagcgtgtt cttgagggcg acctcgaagc tttccgtgtc
     4501 tacgcccacc tcgcgctggg tcacgacgca gcccctgtgc tggtgcacga attcgatggg
     4561 gtcctcgatg ctgatgatgt gacccgagga gtgctcgttg cgatgaccga tcatggcggc
     4621 cagcgacgtg gacttaccgg tgccggtcgc ccccacgaaa atgatgaggc cgcgccgcgt
     4681 catcgacagg tcctccagga tgggcggcag gcccagatcg gagaccagag gg
//
LOCUS       DBNHOX010000494         1534 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_746592, whole genome shotgun sequence.
ACCESSION   DBNHOX010000494 DBNHOX010000000
VERSION     DBNHOX010000494.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_746592"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="AC16UZ_10865"
     CDS             complement(<1..527)
                     /locus_tag="AC16UZ_10865"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR6247227.1"
                     /translation="MENRRRYPRYPISLSALMHPAQGRSWLCTISNFCRDGMLLVGNG
                     GPGSLDATGADAKPGDTVALHFSVALPGGQKHFRTQARVARILGSGEGMGVHFPGGLP
                     DDAYDTLMAFEAAGNPSGENSQPLDEDPPDDVESPQAPVAPQRISHEDADEIGRKMRR
                     ITQPTLDALCQKCFEL"
     gene            complement(596..>1534)
                     /locus_tag="AC16UZ_10870"
     CDS             complement(596..>1534)
                     /locus_tag="AC16UZ_10870"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR6247228.1"
                     /translation="LWSVVLAALVVFDVPSARFDRGYLTFFKALWCNVGVVATALVVP
                     ASLRPLMLVVPLFGVLYTALHLERKHVLAVGTVTWFTYVLGALIQNDMRFIDETSPGL
                     STEGMVVVAFALMLIVMTVMGGEVTALRNAFAHRRERLKQAMERLSELAMRDELTGLY
                     NRRYIMDALAQQKAVADRGRLGFAVLYCDLDHFKRINDRFGHQSGDRALCDFGKLASS
                     VVRSIDYVARIGGEEFLLVLVGADINDATIVAERLCKRTRNLPISAQGGEYHLTVSIG
                     VSSFRSGERVEDVIQRADLAVYRAKSGGRDQISVLV"
BASE COUNT          292 a          499 c          479 g          264 t
ORIGIN      
        1 agctcgaagc acttctgaca gagcgcatcg agggtcggct gggtgatccg acgcatcttg
       61 cgccctattt catcggcatc ctcgtgtgag atgcgctggg gggcgaccgg ggcttgcggg
      121 ctctccacat cgtccggagg gtcttcgtcc aggggctggc tgttctcccc ggacgggttg
      181 cctgcggcct cgaatgccat cagggtatcg taggcatcgt cgggcagacc gccggggaag
      241 tgaacgccca tgccctcgcc cgaacccagt atgcgggcta cgcgagcctg ggtgcgaaag
      301 tgcttctgac ccccgggcaa agccaccgag aagtgcagcg ccacggtgtc gcccggcttg
      361 gcgtccgccc cggtggcgtc gagcgatccg ggcccgccgt tgcctaccag aagcatcccg
      421 tcacgacaga agttgctgat cgtgcacagc cacgatctgc cctgagccgg atgcatcaga
      481 gcagaaagcg agatcggata cctgggatag cgtcgccgat tttccaaaac tgcagcgtcc
      541 ttctgcgagg aggccctacc tctttgctag cctagttact gttgttctca tccgctcaca
      601 ccaacacgct gatttggtca cggccgcccg atttcgcccg gtagacggcc agatctgcgc
      661 gctggatcac atcctccaca cgctcacccg agcgaaagct cgacacgccg atggatacgg
      721 tcaggtggta ttcgccgccc tgagcggaga tcggcaggtt gcgcgtgcgt ttacacagcc
      781 gctcggccac gatcgtggcg tcgttgatgt cggcaccgac gagcacgagc agaaattctt
      841 cgccgccgat cctcgcaacg tagtcgatgc tgcggaccac gctggatgcc agcttgccga
      901 aatcacacag cgcccggtct ccgctttggt ggccgaaccg gtcattgatc cgcttgaagt
      961 gatccaggtc gcagtagagc acggcaaagc ccaggcggcc gcggtcggca acggccttct
     1021 gctgggccag cgcatccatg atgtaacgcc ggttgtacaa gccggtgagc tcgtcgcgca
     1081 tggccagctc agatagcctt tccatggcct gcttgagccg ctcgcggcgg tgggcgaacg
     1141 cattgcgcag agccgtcacc tcaccaccca tgaccgtcat cacgatgagc atgagggcga
     1201 aggcgaccac caccatgccc tccgtggaga ggccgggcga ggtctcgtcg atgaagcgca
     1261 tgtcgttctg gatcagcgcg ccgaggacat aggtgaacca ggtcaccgtc cccaccgcga
     1321 ggacgtgttt gcgctcgaga tgcagcgccg tgtagagcac gccgaacagc ggcacgacca
     1381 gcatcagggg ccgcagggaa gccggaacga caagggcggt cgcaaccaca cccacgttgc
     1441 accacagcgc tttgaagaac gtcaggtatc ccctgtcgaa ccgtgcgctg ggcacgtcga
     1501 aaaccaccag agccgccagg acgacgctcc agag
//
LOCUS       DBNHOX010000495         1583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1646508, whole genome shotgun sequence.
ACCESSION   DBNHOX010000495 DBNHOX010000000
VERSION     DBNHOX010000495.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1646508"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="AC16UZ_10875"
     CDS             complement(<1..195)
                     /locus_tag="AC16UZ_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247229.1"
                     /translation="MSDSIMVTQGSLDREAELDELEVRVREALRLASQAGATEAEVSA
                     HSSLGLSVNVRLGEVETLEHM"
     gene            234..773
                     /gene="yjgA"
                     /locus_tag="AC16UZ_10880"
     CDS             234..773
                     /gene="yjgA"
                     /locus_tag="AC16UZ_10880"
                     /inference="COORDINATES: protein motif:HMM:NF016626.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HIR6247230.1"
                     /translation="MPKGKENIGVDADIPSKSQRRREALEVRSLAARLIGLRPALLAQ
                     VPLDERVSEAISEAQRIRSNVARKRQLQFVAKLLRKTDLEPIAQALQDFERAARQTSA
                     RQHRAEAWRDCLLAGGDPALGELLHQRHDSDAQLIRQLIRTARQEADRGRPPSAARAL
                     FRALREMDEREPLPAMPEG"
     gene            complement(783..>1583)
                     /locus_tag="AC16UZ_10885"
     CDS             complement(783..>1583)
                     /locus_tag="AC16UZ_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016781785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HIR6247231.1"
                     /translation="LLEGDRWRHMADEAVRQALVNLDSVAAPAGTMTVVLGPGWPGVL
                     LHEAVGHGLEGDFNRKGTSAFTGRMGEKVAAECVTVVDDGTIARRRGSLTIDDEGTPT
                     RHNVLIENGVLRGYLQDKLNARLMNMAPTGNGRRESFAHLPMPRMTNTFMLPGAHEPG
                     EIIASVERGLYAVNFAGGQVDITSGRFVFSASEAYLIEKGKVTRPVRGATLIGNGPDV
                     LTRVSMVGKDLELDAGIGVCGKDGQSVPVGVGQPTLRIDGLTVGGTDG"
BASE COUNT          278 a          552 c          496 g          257 t
ORIGIN      
        1 catgtgttcg agcgtttcga cctcgcccag gcgcacattg accgacaggc cgaggctgct
       61 gtgggcgctt acctcggcct ccgttgctcc ggcctggctg gcgagccgca gtgcttcgcg
      121 aacgcgcact tcgagctcat cgagctccgc ttcacggtcc agcgatccct gcgttaccat
      181 gatggaatca gacattgcgt tttcccttct tgacgagaat cttggtgaac cccatgccaa
      241 aaggcaagga aaatatcggt gtagacgcgg acattccgag caagagtcag cggcgccgcg
      301 aggccctcga ggtcagatcg ctggcggccc gtctgatcgg gctcaggccg gccctgctcg
      361 cgcaggtgcc tctcgatgaa cgggtaagcg aggccatctc cgaagcgcag cgcatacgct
      421 ccaacgtggc ccgcaagaga cagctccagt tcgtcgccaa gctgctcagg aaaaccgacc
      481 tggaaccgat cgcccaggcg ctgcaggact tcgagcgggc ggccaggcaa acgagcgccc
      541 ggcagcaccg ggccgaagca tggcgcgact gcctgctcgc aggcggcgac ccggccctcg
      601 gcgaactgct tcatcagcgg cacgacagcg acgcccagct catccgtcaa ttgattcgca
      661 cggcccgcca ggaagcggac cgcggccggc cgccatccgc cgcccgcgcg ctctttcgcg
      721 cgttgcggga aatggacgaa cgcgaaccat tacctgcgat gccggaaggt tagcccgaat
      781 ggctagccgt ccgtgccacc cacggtcagg ccatcgatgc gcagcgtggg ctggccaaca
      841 ccgaccggca cgctctggcc atccttgccg cagacgccga tgccggcgtc gagttccaga
      901 tccttgccca ccatggacac gcgggtcagc acatcggggc cgttgccgat cagcgttgcg
      961 cccctgaccg ggcgagtgac cttgcccttt tcgatcaggt acgcctcgct ggcggaaaac
     1021 acgaagcgcc cggaggtgat atccacctgc cccccggcaa agttgacggc atagaggcct
     1081 cgctccaccg aggcgatgat ctcgccgggc tcatgggctc cgggcaacat gaaggtgttc
     1141 gtcattcgcg gcatgggcag atgcgcaaac gactcgcgac ggccgttacc cgtgggcgcc
     1201 atgttcatca ggcgggcgtt cagcttgtcc tggaggtaac cacgcaaaac gccgttttcg
     1261 atcaggacat tgtgtcgcgt gggcgtgccc tcgtcatcga tcgtcaggga gccgcggcgc
     1321 cgggcgatcg tgccgtcgtc cacgacagtg acgcattccg ccgccacctt ctcccccatg
     1381 cggccggtaa aggccgaggt gcccttgcgg ttgaagtcac cttccaggcc gtgcccgacg
     1441 gcctcgtgga gcaatacgcc cggccagccg gggcccagga ccaccgtcat cgtgcccgcc
     1501 ggggccgcga ccgaatcgag gttgaccaac gcctggcgca ccgcttcgtc ggccatgtgc
     1561 cgccagcggt cgccctccag caa
//
LOCUS       DBNHOX010000496         2326 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_939862, whole genome shotgun sequence.
ACCESSION   DBNHOX010000496 DBNHOX010000000
VERSION     DBNHOX010000496.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_939862"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="AC16UZ_10890"
     CDS             <1..861
                     /locus_tag="AC16UZ_10890"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR6247232.1"
                     /translation="ANAVMHYAERACRAARLSGRNQIKEFEIGEIAQLRRHGEAAWAF
                     RLDEAMAGEGLIVSFRQIAPMSGARNGVDTHYEVQLTMRDEEGGIMAPEKFQDAAESH
                     ERMPALDRWTVAWCLDWMAAHKDSLDHFGGFAIRLSDRSLNEPTFSDFVLERFSACQV
                     PTSKICFEITESVAVANIENAVEFMNRMKIIGCRFALEDFGSGRASYAYLRRLPVDYV
                     KIDDVFVREIAANPGDYAVVRSINEIGHYMGKKTIAEFVEDEEVRARLAEIGVDYLQG
                     MDAQSAIPLR"
     gene            complement(888..1808)
                     /gene="argF"
                     /locus_tag="AC16UZ_10895"
     CDS             complement(888..1808)
                     /gene="argF"
                     /locus_tag="AC16UZ_10895"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR6247233.1"
                     /translation="MAKRDFLTLLDCSGDELSALVARGRELKQNLARRDRPLLGHTAV
                     LILALNSTRTRVGFETGMNQLGGHTVVLNPEDTQLGRGEPIEDMARVLSEMVDVIIIR
                     TLEQTDIERFARAASVPVINAMSAKYHPCQLLADVQTFEELRGPIAGRTVAFVGDGHN
                     MCHSYINAARQWGFNLKIACPEGYSPDPSVLGSTNRASLVESAREAVEGADLVVTDVW
                     SSMGQEEEAARRREQFAPFQVNETLLDHAPDHTVFMHCLPAHRGEEISDKLLDDPRSA
                     VWQEAGNRLHSQKALLEFLILGEFPGSSGG"
     gene            complement(1827..>2326)
                     /locus_tag="AC16UZ_10900"
     CDS             complement(1827..>2326)
                     /locus_tag="AC16UZ_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6247234.1"
                     /translation="RTGRYFAYQHADVLPDVVTTAKGLGNGVPIGACLARGQAAELLV
                     PGTHGSTFGGNPLVCAAAHAVLDALTEDGLIERAASLGERIAAGLKQSLAGNNRVVDI
                     RGRGLMIAVQLNEPCGRLVKAGLEAGILINVTRDDTVRLLPPLTLSDAEADQLVELVS
                     GLVSA"
BASE COUNT          392 a          733 c          795 g          406 t
ORIGIN      
        1 gcaaatgcgg tcatgcacta tgccgaaagg gcatgccggg cggccaggct gtcgggtcgc
       61 aatcagatca aggagttcga gatcggggag attgcgcagc tgcggcgcca cggggaagcc
      121 gcgtgggcct tcaggctgga tgaagccatg gccggtgagg gccttatcgt gagtttccgc
      181 cagatcgccc cgatgagcgg cgcgcgcaac ggcgtcgata cgcactacga agtgcagctg
      241 accatgcgcg acgaggaagg tgggatcatg gcccccgaga aattccagga tgcggcggaa
      301 agccacgaac gcatgcccgc cctggaccgc tggactgtgg cctggtgtct cgactggatg
      361 gcggctcaca aggacagcct ggatcatttc ggcggtttcg cgatccggct gtcggatcgc
      421 tcgctgaacg agccgacgtt ctcggacttc gttctggagc ggttctccgc ctgccaggtc
      481 ccgaccagca agatctgctt cgagatcacc gagtcggtgg cggttgccaa catcgagaac
      541 gcggtggagt tcatgaaccg catgaagatc atcggctgcc gtttcgccct ggaggacttc
      601 gggtcgggcc gcgcgtccta tgcctacctg cgccgcctgc ccgtagacta cgtgaagatc
      661 gacgacgtgt tcgtccggga gatcgcggcc aatcccggcg actacgccgt ggttcgctcg
      721 atcaacgaga tcggtcacta catgggcaag aagaccatcg cggagttcgt cgaggacgag
      781 gaggtccgcg cccggctggc cgagatcggg gtggactacc tgcagggcat ggatgcccag
      841 agcgcgatcc cgctgcggta atcgcccagc cgtcctccgg cttgaaatca accgccggac
      901 gagcctggaa attcccccag aatcagaaac tcgagcagcg ccttctggct gtgcaatctg
      961 ttgcctgctt cctgccagac cgcggagcgg ggatcgtcca agagcttgtc gctgatctct
     1021 tcgccccgat gggcgggcag gcagtgcatg aagacggtgt ggtccggagc atggtccagc
     1081 agcgtctcgt tgacctggaa cggcgcgaac tgctcgcggc gccgggcggc ttcctcctcc
     1141 tggcccattg aagaccagac gtcggtgacc accaggtcgg ctccctccac ggcctcccgg
     1201 gcggattcaa ccaggcttgc ccggtttgtg gagccgagca ccgagggatc gggtgagtag
     1261 ccttcgggac aggctatctt caggttgaag ccccattgcc gggccgcgtt gatgtaggag
     1321 tggcacatgt tgtggccatc gcccacgaac gccaccgtgc gccccgcgat gggaccgcgc
     1381 agctcctcga aggtctgaac atcggcgagc agctgacagg gatgatactt cgcgctcatg
     1441 gcgttgatca ccgggacgct ggccgcgcgg gcgaagcgtt cgatgtccgt ctgctcgagg
     1501 gttcgaatga tgatcacgtc caccatctcg gaaagcaccc gagccatgtc ctcgataggc
     1561 tcgccgcggc cgagctgggt gtcctctggg ttgagaacga cggtgtggcc gccgagctga
     1621 ttcatgccgg tctcgaaccc cacccgggtg cgggtcgagt tcagggcgag gatgagcacc
     1681 gccgtgtgcc cgagtaacgg tcggtcacgt cgggcgaggt tttgcttgag ttcccggcct
     1741 cgcgccacga gcgcgctcag ctcgtcgccg gaacagtcga gaagggtgag gaagtcgcgc
     1801 ttggccatgg tggcaggttc cgcgggtcag gcggagacga gaccgctcac cagctctacc
     1861 agctggtcgg cctcggcgtc gctcaaagtc agcggcggaa gcagcctgac ggtgtcgtct
     1921 cgggtcacgt tgatcagaat cccggcctcc aggcccgcct tcaccagcct gccgcatggc
     1981 tcgttgagct gcaccgcgat catcaggccg cgaccccgga tgtcgacgac ccggttgttg
     2041 ccggccaggg attgcttcag gccggctgcg attctttcgc cgaggctggc ggcgcgctcg
     2101 atcagcccgt cttccgtcag cgcgtccagc accgcgtggg ccgcggcgca gaccagcggg
     2161 ttgccgccga acgtggagcc gtgggtgccc gggaccagca gttccgccgc ctgaccacgg
     2221 gccaggcagg caccgatggg cacgccgttg ccgagaccct tggccgtcgt caccacgtcc
     2281 gggagcacgt cggcgtgctg gtaggcaaag tagcggccgg tgcggc
//
LOCUS       DBNHOX010000497         1779 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1582145, whole genome shotgun sequence.
ACCESSION   DBNHOX010000497 DBNHOX010000000
VERSION     DBNHOX010000497.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1582145"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /gene="ccsA"
                     /locus_tag="AC16UZ_10905"
     CDS             complement(<1..634)
                     /gene="ccsA"
                     /locus_tag="AC16UZ_10905"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIR6247235.1"
                     /translation="MLETLELGLFGLATALYALAWGWHLAGWKRGSAAHTGIAVRILW
                     AGWALNVAMVGLRWYRADHVPMLSAFEFVTFFAMLVIGCFLLFALREHNRMLGVFLVP
                     VGVLLMIYAAFMPKAIEPELTIFKGFLLQFHIVTTLLGYAAWAVTFAASICYLYLERK
                     QDKNPGLFDRMAYRAAFFAFAFLTLGIITGALWSDRVFGQLWFWDPKETWS"
     gene            complement(627..1586)
                     /locus_tag="AC16UZ_10910"
     CDS             complement(627..1586)
                     /locus_tag="AC16UZ_10910"
                     /inference="COORDINATES: protein motif:HMM:NF016995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis ResB family protein"
                     /protein_id="HIR6247236.1"
                     /translation="MPGRLPGQVFRLLASLKVAIPLLVLLTGVTIIGSLYPEPDIFRS
                     WWYLALLGVQGVSLLFVTILHIPSILKRKGRNALLGVIATHLGILVIIAGIIYGGYTG
                     VRHQVRLIEGEAKVLPGLPFVIQLDRMVVEEYRQEEFPRQDFSSLPKKRQDSHVTLLK
                     TGKPWISAVAAPGRPVKADGFTLLPSINDLGWYFELIVIDRLGRESTVPVPPWKPPVI
                     DLGERDGMVHGKMLQTDPQAEIFTQEGGELLSLGTVREGEDLEIDGNRYTLGPVKRYT
                     GMQVYNRPQEPILIWGSVLMFAGLLWHFYFRHRDRRREGKSDA"
     gene            complement(1591..>1779)
                     /locus_tag="AC16UZ_10915"
     CDS             complement(1591..>1779)
                     /locus_tag="AC16UZ_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247237.1"
                     /translation="LEINPMDDRERELWEQARGLVDIMASVERRQADGEITAPPAHDV
                     SGHDPMSSEPDPEPQGDD"
BASE COUNT          351 a          591 c          533 g          304 t
ORIGIN      
        1 aggaccaggt ctccttcgga tcccagaacc agagctggcc gaacacacga tcgctccata
       61 acgcgcccgt aatgatgccg agcgtgagaa acgcgaaggc gaagaacgcc gcccggtagg
      121 ccatgcgatc gaacaggccc gggttcttgt cctgcttgcg ctcgaggtac aggtagcaaa
      181 tcgacgcggc aaaggttacg gcccaggcgg cgtagccgag cagcgtcgtc acgatgtgga
      241 actgcagcag gaagcccttg aagatcgtca gctccggctc gatcgccttc ggcatgaaag
      301 cggcgtagat catcagcagc acgccgaccg gcacgaggaa cacgccgagc atgcggttgt
      361 gctcgcggag cgcgaacagc aggaagcagc cgatcaccag catcgcaaag aaagtcacga
      421 actcgaacgc gctcagcatc ggcacgtgat ccgcccggta ccagcgcagc ccgaccatcg
      481 cgacattcaa cgcccagccg gcccagagaa tgcgcaccgc aatgcctgta tgcgccgctg
      541 agccgcgctt ccagcccgcg aggtgccatc cccaggccag tgcatacagc gctgtcgcca
      601 gcccgaagag ccccagttcg agtgtttcaa gcatcgctct tgccctcgcg ccggcgatcg
      661 cggtgccgga aatagaagtg ccacaggagc ccggcgaaca tcagcacact gccccagatc
      721 aggatcggtt cctgcggccg gttgtagacc tgcatgccgg tgtagcgctt tacgggcccg
      781 agagtgtacc tgttgccgtc gatctccagg tcttctccct cgcgtacggt gccgagggat
      841 agcagctcgc caccctcctg cgtgaagatt tcggcctgag ggtccgtctg cagcatcttc
      901 ccgtgcacca taccgtcacg ttcgcccagg tcgatcacgg gcggcttcca gggcggcacg
      961 ggaaccgtgc tctcgcggcc cagccggtcg atgacgatca gctcgaaata ccacccgagg
     1021 tcgttgatgg acggcagcag cgtgaagcca tcggccttta cgggccgccc gggcgccgcg
     1081 actgccgata tccatggctt gcccgtcttc aacagggtga cgtggctgtc ctggcgcttt
     1141 ttcggcaatg acgagaagtc ctggcgaggg aattcttcct gacggtattc ctcgaccacc
     1201 attcgatcga gctggatcac gaacggcaag cccggcagga ccttcgcctc tccctcgatc
     1261 agcctgacct ggtgccggac acccgtgtaa ccgccgtaga tgatgcccgc gatgattacc
     1321 aggattccga gatgcgtcgc gatcacgccg agcaatgcgt tgcgtccctt gcgcttgagg
     1381 atcgagggaa tgtgcaggat ggtgacgaac agcaaactga caccctgcac gccgagcagc
     1441 gccaggtacc accacgacct gaaaatatcg ggctcgggat acaggctgcc gatgatggtc
     1501 acgcccgtga gcagcaccag cagcgggatg gccactttta acgaggccag caggcggaac
     1561 acctgccctg gcagtcttcc cggcaccagc ctaatcgtcc ccttgcggct cgggatcggg
     1621 ctccgacgac atgggatcat gaccgctgac gtcatgcgcc ggcggcgcgg ttatctctcc
     1681 gtctgcctgc cgccgctcga cgctcgccat gatgtcgacg agaccccggg cttgctccca
     1741 caactcccgt tccctgtcgt ccatgggatt gatttccag
//
LOCUS       DBNHOX010000498         2325 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1133400, whole genome shotgun sequence.
ACCESSION   DBNHOX010000498 DBNHOX010000000
VERSION     DBNHOX010000498.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1133400"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="AC16UZ_10920"
     CDS             complement(<1..264)
                     /locus_tag="AC16UZ_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247238.1"
                     /translation="MIRFNRHLSAAALVLFSGAARAEVVDLPVPFLEWSFYILLLFGL
                     VVAIGIFIHRGKGGTAEPLSKMMQERSAAVHSVTPDVTVTECVR"
     gene            complement(297..1463)
                     /gene="nrfD"
                     /locus_tag="AC16UZ_10925"
     CDS             complement(297..1463)
                     /gene="nrfD"
                     /locus_tag="AC16UZ_10925"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR6247239.1"
                     /translation="MDAAPFTGYVYPNEGVIQWSVLIVIYPYLTGLVAGAFTVSSFYH
                     VFGMQRFKPVARLALLTSLCVMIFVPLPLLMHLGHPERSYYSMITPHLTSAFAMFGFF
                     AAFYMVLLTLECWFVFRKDNVERARSSTGLKRAFYRAVTLWSDDVSPRALSIDANILL
                     LLAIVGIPSAHGLHGYIGFVYGSLKARLWWESDLMPVIFLFSAIVSGIALLMLLYVVS
                     CKLRRVIIDHDCLKGMTYALWGFLMFTVVLEGLTFGNLVYKGREGVEVTMQYVQGPLF
                     FRYFVLQFFVGALVPIGIIGVMMAWNIRGRLFVIGATVSSLLVLLNVLMMRWNVVIGG
                     QEIGKSAKGLLTYDMPILGHESALTAALLTLAPFALLAVLVRLFPPWADPPQEA"
     gene            complement(1463..2278)
                     /locus_tag="AC16UZ_10930"
     CDS             complement(1463..2278)
                     /locus_tag="AC16UZ_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6247240.1"
                     /translation="MTDETKKRGRREFLSATGKALAGLGVAGCAGSLLGQDEGKESFP
                     TPDYDWTEHRWAYGIDATKCIGCLRCVEACKAENDVVRDAVHFRTWVERYVYLEGEEH
                     ARVDSHNDPQNIAASGSEAEYRFDNRYKDAKVRKAFFVPKICNHCTHPACVQVCPVGA
                     TFRTEDGVVLIDHDYCIGCRYCVQACPYGARSFIEEEGVSDKCTWCYHRITKGLQPAC
                     VEVCPTHARVFGNLNDRSSVISQFVDNNQVQVLKPESGNGPMCFYIGIDKDVS"
BASE COUNT          466 a          741 c          741 g          377 t
ORIGIN      
        1 ccggacgcat tccgtgaccg tgacatcggg cgttaccgag tggaccgccg cgctgcgctc
       61 ctgcatcatt ttgctcagcg gctcggccgt cccgcccttg ccgcggtgga tgaaaatccc
      121 tatcgcaacg accaggccaa acagcagaag aatgtaaaac gaccattcca ggaacggcac
      181 cggcaggtcg accacctcgg cgcgggcagc gccggaaaac agcaccaggg ctgccgcgga
      241 caaatgtctg ttgaatcgaa tcatctcgat gcctcccccg gaccgcgcgg cccgggtcat
      301 gcctcctgcg gtggatctgc ccacggtggg aacaggcgca ccagcacggc cagcagcgcg
      361 aagggcgcga gcgtcagcag cgccgccgtg agcgcgcttt cgtgtcccag gatgggcatg
      421 tcataggtca acaggccctt ggccgacttg ccgatctcct ggcccccgat gaccacgttc
      481 cagcgcatca tcagcacatt gagcaggacc agcagggacg acacggtggc gccgatcacg
      541 aacaaccggc cgcgaatatt ccaggccatc atcacgccga tgatgccgat cggcaccagc
      601 gcgccgacga agaactgcag cacgaaatag cggaagaaaa ggggcccctg cacatattgc
      661 atggtcacct cgacgccctc gcggcccttg tagaccaggt ttccgaaggt caggccttcc
      721 aggaccaccg tgaacatcag gaaaccccac agggcatagg tcatgccttt gaggcagtcg
      781 tgatcgatga tcacgcggcg cagcttgcaa gagacgacgt acagcagcat cagcagcgca
      841 atgccggaga cgatggccga aaacaggaaa atcaccggca tcaggtcgga ttcccaccac
      901 aggcgggcct tcagcgagcc gtacacgaaa ccgatgtagc cgtgcaggcc gtgcgccgac
      961 ggtatgccga cgatggcgag cagcagcagg atgttggcgt cgatcgaaag ggcccgcggc
     1021 gagacatcat cggaccacag tgtcacggcc cggtaaaagg cccgcttcag tccggtcgaa
     1081 ctccgcgccc gctcgacgtt gtccttgcgg aacacgaacc agcactccag cgtcagcagt
     1141 accatgtaaa acgccgcgaa aaatccgaac atggcgaacg ccgaggtcag atgcggcgtg
     1201 atcatcgagt aataggagcg ctccggatgc cccaggtgca tcagcagcgg cagcggcacg
     1261 aagatcatca cgcacagcga ggtgagcagc gccagccggg caaccggctt gaagcgctgc
     1321 atgccgaaca cgtggtaaaa gctggaaacc gtgaatgcgc cggcgaccag gcccgtcagg
     1381 tacgggtaga tcacgatcag cacgctccac tggatcacgc cctcgttggg gtagacatag
     1441 ccggtgaacg gtgcggcgtc catcacgata cgtccttgtc gatgccgata taaaagcaca
     1501 tggggccgtt gccgctctcg ggcttgagca cctgcacctg gttgttgtcg acgaactggc
     1561 tgatgacgct cgagcggtcg ttgaggttgc cgaacacgcg ggcgtgggtg gggcagacct
     1621 cgacgcaggc cggctgcagc cccttggtga tgcggtgata gcaccaggtg cacttgtccg
     1681 agacgccttc ctcttcgatg aacgagcgcg cgccataggg gcaggcctgc acgcagtagc
     1741 ggcagccgat gcagtagtcg tggtcgatca gcaccacgcc gtcctcggtg cggaaggtcg
     1801 cgcccaccgg gcacacctgg acgcaggccg ggtgcgtgca gtggttgcag atcttcggca
     1861 cgaaaaaggc ctttctgacc ttggcgtcct tgtagcggtt gtcgaagcgg tactccgctt
     1921 cggaacccga ggccgcgatg ttctgcgggt cgttgtggct gtcgacgcgc gcgtgttcct
     1981 cgccctcgag gtagacgtag cgctcgaccc aggtgcggaa gtgcacggcg tcgcgcacca
     2041 cgtcgttctc ggctttgcag gcctcgacac agcgcaggca gccgatgcac ttggtggcat
     2101 cgatgccgta ggcccagcgg tgctcggtcc agtcgtagtc aggcgtgggg aaggattctt
     2161 tcccctcatc ctgaccgagc aggctgccgg cgcaaccggc cacgccaagg ccggcgagcg
     2221 ccttgcccgt ggccgagagg aactcccgcc gcccgcgttt cttggtctcg tcggtcatta
     2281 tttgtctccc ccgggctcat cggtcggctg cggcggcgat gggct
//
LOCUS       DBNHOX010000499         4894 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1389802, whole genome shotgun sequence.
ACCESSION   DBNHOX010000499 DBNHOX010000000
VERSION     DBNHOX010000499.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1389802"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..996
                     /locus_tag="AC16UZ_10935"
     CDS             112..996
                     /locus_tag="AC16UZ_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6247241.1"
                     /translation="MKIRSAMVVMAKPKHKQFALERAKQLQQSCGARLRLVSFCWNAM
                     CEQSDVFEAHQRRAIKATIMRERESWLIDQVRDAGMAAADVSIEVQWTDNIAGWVSEK
                     AAANEFDLVLKSVNRSRTLVHTPLDWQLLEQCSKPVYISAPPRRSASGNVLAALDFRH
                     RDRKHQALNIRVLEAAQEFADMSGGKLHCVNVVEFSQVLSDLDLIDPRSVKKKALANN
                     QELMNALLEPFDVPKSRMHTPLGKVGQMIAITARKIDADLLVIGTSTRRKGGTVVIGN
                     SAQRTLEKSPCDLLAVHP"
     gene            complement(1076..1492)
                     /locus_tag="AC16UZ_10940"
     CDS             complement(1076..1492)
                     /locus_tag="AC16UZ_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6247242.1"
                     /translation="MLNTLKLILALAATCYFGLAAADDIAYDGGELYQANCANCHGMY
                     GEGDGIVTPSLSVVLQDLRYLAERNDGEFPRWFVTEIIDGRELRLAHGPDGMPVWGEA
                     FTRMEGYGEQAQSRVEQKIDALVNFIESIQIAPEDE"
     gene            complement(1495..3540)
                     /locus_tag="AC16UZ_10945"
     CDS             complement(1495..3540)
                     /locus_tag="AC16UZ_10945"
                     /inference="COORDINATES: protein motif:HMM:NF014312.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HIR6247243.1"
                     /translation="MTPAKPIKATLIGLFAVCPLISPMVAHPNTAEQEKTGKELYEQQ
                     CARCHQRDGGGVDGLYPSLRNAPSLWLDRKTAIRGVLAGRADDTVVNSPLMPTHGYLG
                     NETVASTLTFLLQEWGPGGQPYTAEEVAAERLELLASQPAPHPGLPDVSPLADMDAVQ
                     YVTSSGPPMSVSEFDRARSLYYGHCTGCHGVLREGTAGSPLTPVLMRQRGTEYLQAVI
                     NYGSSAGMFNWGTSAALTAEEINMLARFLQHPVPQAPDMTEADVRASWSLEVPLKDRP
                     GVPQGRHPLDELFVVTLHDVAQIALIHGPSRRVIGTVATGQSPHRVRGSASGRYLYVI
                     ARDGSLTMIDLYARKPRRVASVRVGYEARAVGASRHPDYADRYVLAGAYWPPQLVLLD
                     GRTLEPLKLISTRGRTADGGRYHPEPRVSDIAGSYLRPEFIANVKETGRVYLFPYYAT
                     DRLQLVDIGTTAELRAGTFSQDKRYFITPADVNAISVVDTKEQRQVAQIPARVFAGGP
                     GVTYDDPEYGPVWSTSTMVEDEIISIGTDPEGHPNYAWEKVRSLKGPGSGSMFMASHP
                     ASTNLWVDSPLNADETVSQSVAVFDKRDLDAGYRSIDVAAIADVGEGPSRVLQPTYDP
                     TGREVWVVVWNPQDLNSAIVVIDDRTLKPLAVIDDPRLITPTRIYSLAALRDAPSEN"
     gene            3673..>4894
                     /locus_tag="AC16UZ_10950"
     CDS             3673..>4894
                     /locus_tag="AC16UZ_10950"
                     /inference="COORDINATES: protein motif:HMM:NF025052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside
                     phosphotransferase/ATP-binding protein"
                     /protein_id="HIR6247244.1"
                     /translation="MAQPELVEHLLRPEAYPHPCRAVRLVETHISWVFLTGDYAYKVK
                     KPVLFSFVDFSTARLRKHYCEEELRCNRAFSPELYLDVVEIVRDVEGRIRVGGTGEVV
                     EHAVKMRQFPQSSALDRLLVAGVLEPGGLSRFGLELATLHAGLPRLEAPDDELDRRIL
                     GPVLDNFATLRATRAGGDRSDILQRLESASKDAFDGKRTTFSRRLAKGFVRECHGDLH
                     LRNLVMIDGRVRAFDCLEFNPDLRWIDIASDAAFLIMDCAVRDRVDLAYAFQDGYLAG
                     CGDYVGATLSPFYQAYRSVVRAKVAALEIEGSPKDGKLGAARERLDRHLNWAWERLHG
                     PRGRMVLMCGLSGSGKSYVAERLVPRLPAVRIRSDVLRKVAAGLEPLQRSGSSVDAGL
                     YTPSRSQSVYAEMQA"
BASE COUNT          850 a         1463 c         1640 g          941 t
ORIGIN      
        1 cgggggctgc ttctgggcaa cacctcggag cgcatacttc atgcggttgc cacggacgtg
       61 ctgaccatgc atggtaccga ttaaacgaaa gcgatcgacg ggagaacggg tttgaagatc
      121 aggtcggcca tggtggtgat ggcgaagccc aagcacaaac agtttgctct ggagagggcc
      181 aagcagctgc agcagagttg tggcgcccgg ctgaggctgg tctccttctg ctggaacgcc
      241 atgtgcgagc agtcggacgt gttcgaggcg catcagcgcc gtgcgatcaa agctacgatc
      301 atgcgtgagc gtgagtcttg gctgatcgat caggtccgcg atgcaggtat ggcagccgcg
      361 gacgtcagca tcgaggttca gtggaccgac aacatcgcag gctgggtcag cgagaaagcg
      421 gcggccaacg agtttgatct ggtgctcaag tccgtcaatc gctcccggac gctcgttcac
      481 acgccgctgg actggcagct cctggagcag tgttccaagc ctgtctacat atcggcgccg
      541 ccgcgccgct cagcgtcggg taatgtcctc gccgccttgg atttccgtca tcgcgacagg
      601 aagcatcagg ccctcaacat ccgtgttctg gaggcggccc aggagtttgc cgacatgtcg
      661 ggcggcaagc tgcactgtgt gaacgtggtg gagttttcac aggtgttgag cgaccttgac
      721 ctgatcgatc cgcgcagcgt caagaagaag gcgctggcca acaaccagga gctcatgaac
      781 gcgctgctgg agccgttcga tgtgcccaag tcgaggatgc acacgccgct gggcaaggtc
      841 ggtcagatga ttgcgatcac cgcgcgcaag atcgatgcgg atctgctcgt gatcggtacg
      901 agcacccggc gcaagggtgg caccgtcgtc atcgggaatt ccgctcagcg aacgctcgag
      961 aaatcgccgt gcgacttgct ggccgtccac ccgtagccag gtaacgatcc ggcagtattg
     1021 ctctcatccg gtcatgaaac agccgggacc gaggcgtccg caaaggcatg acgggtcatt
     1081 cgtcttccgg agcgatctgg atggattcga tgaaattcac cagcgcgtcg attttttgct
     1141 cgacgcggct ctgggcttgc tctccgtagc cttccatgcg ggtgaaggcc tctccccaga
     1201 cgggcatgcc gtctggtccg tgggcaagcc tgagctcgcg gccatcgatg atctccgtga
     1261 cgaaccagcg gggaaactcg ccgtcattgc gctcggccag gtagcgcaga tcctggagga
     1321 cgaccgacag agacggcgtc acgatgccgt cgccctcgcc gtacatgccg tgacagttgg
     1381 cgcagtttgc ctggtagagc tcgccgccgt cgtaggcgat gtcgtcggcg gcagccagac
     1441 cgaagtagca ggttgccgcc agggccagga tcagcttgag agtgttgagc acgttcagtt
     1501 ctccgatgga gcgtcccgca aggcggcgag actgtagatt ctggtgggcg tgatcagccg
     1561 gggatcgtct atcacagcca gcggcttcag ggtacggtca tcgatgacca cgatggcgga
     1621 gttcaggtcc tgggggttcc agacgaccac ccacacctct cggccggtcg ggtcgtaggt
     1681 cggctgcagg acgcggcttg gtccctcgcc gacgtcggcg atggcggcga cgtcgatgct
     1741 gcggtagccg gcatccaggt cgcgtttgtc gaacacggcg acggactggc tgacggtctc
     1801 gtcagcgttg agcggtgaat cgacccagag gttggtggat gcaggatggc ttgccatgaa
     1861 catcgagccg gacccgggtc ctttcagcga tctcaccttc tcccaggcgt agttggggtg
     1921 accctcggga tcggtgccga tgctgatgat ctcgtcctcg accatggtgc tcgtggacca
     1981 gacgggcccg tactccggat cgtcgtaagt cacgccgggc ccgccggcaa acacccgggc
     2041 gggtatctgt gccacctggc gctgttcctt ggtgtctaca acgctgatgg cgttgacgtc
     2101 ggccggcgtg atgaagtagc gcttgtcctg agaaaacgtg cccgccctga gttccgccgt
     2161 ggtgccgatg tccaccagct gcaggcggtc ggtggcgtag tagggaaaca ggtagacccg
     2221 gcccgtctcc ttcacgttgg cgatgaactc ggggcgcagg tatgagcctg cgatgtcgct
     2281 gactcgtggc tccggatgat agcggccgcc atcggccgtt cggcctcggg tggagatcag
     2341 cttcaggggt tcaagcgtgc ggccgtccag gagcacgagc tgaggaggcc agtaggcgcc
     2401 ggcaaggacg taacgatccg cgtaatcggg atggcgtgaa gcgccaacgg ctctcgcttc
     2461 gtagccgacc cttacgctgg ccaccctgcg cggcttcctg gcgtacaggt cgatcatggt
     2521 gagcgatccg tcccgggcga tcacgtagag atagcggccg ctggcggatc cgcgaacccg
     2581 gtgcgggctc tggcccgttg ccaccgtgcc gatgacgcgt cgcgaaggac cgtggatcag
     2641 cgctatctga gcaacatcgt gcagggtcac cacgaacagc tcatccagcg gatggcgccc
     2701 ctggggcacc cctggacggt cctttagagg tacctccaaa gaccagctcg cgcgcacgtc
     2761 tgcctcggtc atgtcgggag cctgaggcac cgggtgctgc agaaagcgag cgagcatgtt
     2821 gatctcctcg gctgtcagcg cggccgaggt gccccagttg aacattcccg cggatgaacc
     2881 gtagttgatg accgcctgaa ggtattcggt cccgcgttgg cgcatcagca ccggcgtcaa
     2941 tggcgatccc gcagtacctt cgcggagcac gccgtgacaa cccgtgcagt gcccgtagta
     3001 cagagatcgc gcccggtcga actcgctcac ggacatggga gggccgctcg acgtcacgta
     3061 ctgcaccgcg tccatatcgg ccagcgggct cacatcgggc agtcccgggt ggggggccgg
     3121 ttggcttgcc agcagctcga gtcgctcagc ggcgacctcc tcggcggtgt aaggctgccc
     3181 ccccggcccc cattcctgca gcaggaacgt cagggtactg gcgacggtct cgttgccgag
     3241 atagccgtgg gtcggcatga ggggggaatt gaccacggtg tcgtcagcgc gtccggcaag
     3301 caccccacga atcgccgttt tccggtcgag ccaaagggag ggagcatttc tcaggctcgg
     3361 atagagcccg tctacgccgc ccccgtcgcg ctggtggcat cgggcgcact gctgctcgta
     3421 gagctccttg cccgtctttt cctgttctgc cgtattgggg tgggctacca tcggagagat
     3481 gagcgggcag acggcaaaca agccgatcaa cgtggcctta atgggtttag ctggcgtcaa
     3541 gagggtttcc cggcatggca tggtcgtgtc aatgctaacg gttttcggcg acgatttgcc
     3601 tatccgtagt tcccctagtg agcatctggc ggcaggctcg gacgtcagcc ccgcttcggc
     3661 gaggtgttga gagtggccca gcccgagctc gtcgagcatc tgctacgtcc cgaggcttac
     3721 ccgcacccct gtagagccgt tcgcctggtc gagacgcaca tctcctgggt cttcctcacg
     3781 ggagattacg cctacaaagt caagaaaccg gtcctcttca gctttgtgga tttcagcacc
     3841 gctcgtctca ggaaacacta ttgcgaggag gaactccgct gcaaccgggc gttctctccg
     3901 gagctctatc tggacgtggt cgagatcgtc cgggatgtcg agggccggat ccgggttggc
     3961 gggactggcg aggtcgttga gcatgcagtg aagatgcggc agtttcctca gagcagcgcg
     4021 ctggatcggc ttctggtcgc aggcgttctc gagcccggtg ggttgagtcg attcggactg
     4081 gagctggcca ctctgcatgc tgggctgcca cgtctggagg ccccggacga cgagctggac
     4141 cggcggatcc tggggccggt gctggacaac ttcgcgacgc tcagggcaac ccgggccggc
     4201 ggggatcgct cggacatcct ccaaaggctc gagtcggcct cgaaggacgc ttttgacggc
     4261 aagcgaacaa cgttctcgcg ccgcctggcc aagggcttcg tgcgagagtg tcacggcgat
     4321 ctgcacctgc gtaatctcgt catgatcgac gggcgggtga gggcgttcga ctgtctcgag
     4381 ttcaaccccg acctgcgctg gatcgacatc gcaagcgacg ccgctttcct gatcatggac
     4441 tgtgcggtgc gagatcgcgt tgacctggcc tacgcttttc aggacggcta cctggccggc
     4501 tgcggagatt acgtcggggc gacgttgtcc cctttttatc aggcctaccg atccgtcgtc
     4561 cgagccaagg tggctgccct ggagatcgaa gggtcgccga aggacggcaa gctgggcgct
     4621 gcccgggaac gtctcgaccg gcacctgaac tgggcctggg agcgtctgca tgggccccgg
     4681 ggcaggatgg ttttgatgtg cggtctatcc ggctctggaa aatcgtatgt agcggagcgg
     4741 ctcgtgccgc gattgcccgc tgtgcgtatc cgatctgacg tgctccgcaa ggtggcagcc
     4801 ggcctggaac cgctgcaacg atccggatca tcggtggatg cggggctgta cacgccttca
     4861 cggagccaat cggtttacgc cgagatgcag gcaa
//
LOCUS       DBNHOX010000500         4693 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1004511, whole genome shotgun sequence.
ACCESSION   DBNHOX010000500 DBNHOX010000000
VERSION     DBNHOX010000500.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1004511"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1398)
                     /locus_tag="AC16UZ_10955"
     CDS             complement(13..1398)
                     /locus_tag="AC16UZ_10955"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6247245.1"
                     /translation="MAESQPKPGEARCPGISVQDLMDRDSRTVPAYLREHSYAFLGDE
                     DIPFARYTSRDWFDKEIEHMWPRTWQWACREEHLPAPGDYYVYDVGPYSVIVLRDSSG
                     AIRAYRNACMHRGTKLKPSFSTGSADSIRCPYHGWQWNLEGELQSFPCEWDFPHVDPD
                     AVGLSSVRAELWGGFVFINLDPEAVPLREYLHPLPEHVDSSVFEQRYVAIHIQKELNC
                     NWKIASEAFLEAYHVMETHPQLMRTNGDANTQYDFYGDHVNRLINVAGSPSAYLEEPI
                     TEQEVLDLFLVGDREDIGEALVVPEGGTARQVMAEHFRESVNASGGADLSEVCDSEIV
                     DTIAYFVFPNQQFFSGVSFPIVYRFRPLGMSHDRSLFDFVILKTRPNDGSPAPTAEPV
                     RLTADESYTLVPGMDPYVGHVFDQDTGNVQAAQEGAMATDKAGATLGNYQEIRIRHFH
                     QTLGKYVAGEL"
     gene            complement(1472..2233)
                     /locus_tag="AC16UZ_10960"
     CDS             complement(1472..2233)
                     /locus_tag="AC16UZ_10960"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF048554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6247246.1"
                     /translation="MRPIAGMSILVTGASSGLGAGAAAYFAERGARVTISARREDRLA
                     AVKEAIGDAAAMVVGDVTSAADRQRMIDEAVRHGGGLDALISNAGNMYRGPITDLDEQ
                     ALLDIFHSNVVAGMLLTGLAVPELEKRQGAVIFVGSIHTMRAFPGASPYAATKGALQT
                     LTRVLAAELGERGIRVNCVIPGAVPTEIYQRAGLLSDEDARARIAGMASMHPLGRVGT
                     EEELAEAMAYLIQAEWTTGAELVVDGGLGLGATNA"
     gene            complement(2230..2907)
                     /locus_tag="AC16UZ_10965"
     CDS             complement(2230..2907)
                     /locus_tag="AC16UZ_10965"
                     /inference="COORDINATES: protein motif:HMM:NF018038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR6247247.1"
                     /translation="MRNVMLIGGIYHPFEEAAPVLARLLESLGIESVITSDVSEAVAE
                     LESADLFTIYALRWRMLNDEKYVPFRKQWAFELPEEDAQAIENFVEAGGGMLGLHTAS
                     ICFDTWYEFPRLLGGSWRWGETFHPPEGNVEVRAVADHPATRGVTDFGLHDEIYHHLA
                     IGNESKVLLEGRVPDGDWHPISWAHEAAAGRVVYSALGHDGRSLGDPHHARFIRQAVA
                     WLLGEDE"
     gene            complement(2907..3647)
                     /locus_tag="AC16UZ_10970"
     CDS             complement(2907..3647)
                     /locus_tag="AC16UZ_10970"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6247248.1"
                     /translation="MSVRRALVTGAAHGIGAAIATRLGSAGYRVGVLDMDGTAAAEVA
                     AALPDALPLTADVTDADSIDRALDAFGEPPNLLVNNAGIVHFGPVVEESVADFVKVIS
                     VNLIGCYVASRQCAQRMSGGDHIISITSINDRTPAPGSGAYPAAKAGVRQLMRQLAVE
                     LAERGIRANSVCPGFIDAGMSAPFYADPKVRETRARAVPLGRLGTAEDVANAVAFLDS
                     EAGAYVNGHELVVDGGVTHTLLKHLPRD"
     gene            complement(3644..>4693)
                     /locus_tag="AC16UZ_10975"
     CDS             complement(3644..>4693)
                     /locus_tag="AC16UZ_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6247249.1"
                     /translation="LRVFRRDYARHYEREVFPKALRDALEPLRGQPSIDVVAFGHAVS
                     MNLTADFAGIDRSEDPEATAALQRLTKKFGEGATLVHSTRAHDQVRAEVREALAEYRG
                     RFLEPSMARRERLISAWRGSEIPEESLPRDMLTVLLTDRGGLDDDGLAREIAFYLQAG
                     AHSTSNAFAHALWEVLTWLGSDLARRAALAGDALATQRAVHEALRLHPASPVAWRRAL
                     EPIDFSWGLHVEPEDHLVVDIAAANRDPEVFGPEADAFQPGRAVLDRRANPFGLTFGI
                     GVHNCLGRDIDAGLPQDSDTEAGSHQYGTVTLLVHHFLSELDAMFDPADLPVPDEDTS
                     RPNWGRFPVRVRWQT"
BASE COUNT          749 a         1567 c         1580 g          797 t
ORIGIN      
        1 catcgtgtgc tcctaaagct cgccagcaac gtacttgccg agagtctggt gaaagtgacg
       61 gatgcgtatt tcctgataat ttcccagggt cgcgccggcc ttgtcggtgg ccatggcacc
      121 ctcctgagct gcctggacgt tgccggtgtc ctgatcgaaa acgtggccga catagggatc
      181 catgcccggt accagcgtgt acgactcgtc cgccgtcaac cgcacgggct cggccgtggg
      241 tgctggagag ccgtcgttgg gccgcgtttt gaggatgacg aagtcgaaca ggctgcggtc
      301 gtggctcatg ccgagcggcc ggaagcggta aacgatggga aacgataccc cggagaagaa
      361 ctgctggttg gggaagacga agtaggcgat ggtgtccacg atctccgaat cgcacacttc
      421 ggagagatcc gctcccccgg acgcgttcac gctctctctg aaatgctcgg ccatgacctg
      481 gcgagcggtg ccgccctcgg gcaccaccag cgcctcgccg atatcctcac gatcccccac
      541 cagaaacaga tccagcacct cctgctcggt tatcggctcc tcgaggtaag cgctcggcga
      601 gccggcgacg ttgatcagcc ggttgacgtg atcgccgtag aagtcgtact gggtgttcgc
      661 gtcgccgttg gtgcgcatga gctgagggtg ggtttccatc acgtgatagg cttctaaaaa
      721 cgcctccgag gcgatcttcc agttgcagtt cagctctttc tgtatgtgga tggccacgta
      781 gcgttgctcg aagacgctgc tgtccacgtg ctcgggcagg ggatgaaggt actcccgtag
      841 cggaacggcc tcgggatcca ggttgatgaa cacgaagccg ccccagagtt cggcccggac
      901 ggaggacagg cccacggcat cgggatcgac gtgggggaaa tcccactcgc agggaaacga
      961 ttgcagctcg ccctccaggt tccactgcca gccgtgataa gggcagcgga tggagtcagc
     1021 cgaacccgtg ctgaacgagg gcttgagctt cgtcccgcga tgcatgcagg cgttgcggta
     1081 tgcccggatc gcgccgctgc tgtcccgcaa cacgatgacc gagtacgggc cgacgtcgta
     1141 aacgtaataa tcaccggggg cgggcaggtg ctcctcgcga cacgcccact gccacgtcct
     1201 cggccacatg tgctcgatct ctttgtcgaa ccagtcgcgc gacgtgtagc gggcaaaagg
     1261 aatgtcctcg tcgccgagaa acgcgtagct gtgctcgcgc agataggcgg gcaccgttcg
     1321 gctgtccctg tccatcaggt cctgtaccga aatgcccggg cagcgtgcct cacccggctt
     1381 tggctggctc tcggccatcg cctccccctg atttgtcctt atccccagcg gacattttaa
     1441 gtcaccggcg acgatacgcc accactgccc gctacgcgtt ggtggcccct agccccagcc
     1501 cgccgtccac caccagctcg gcccccgtgg tccactccgc ctgaatcagg taagccatgg
     1561 cctcggcgag ctcctcctcg gtgccgaccc ggccgagggg gtgcatggag gccatccccg
     1621 cgatgcgagc ccgcgcgtcc tcgtccgaca gcaggcccgc acgctgatag atctcggtgg
     1681 gcacggcgcc ggggatcacg caattcaccc ggatgccgcg ctcgccgagc tcggcggcca
     1741 gaaccctggt cagggtctgc agcgcgccct tcgtcgccgc gtagggcgaa gcgccgggga
     1801 atgccctcat ggtgtggatg ctgccgacga agatcaccgc gccctggcgc ttttccagct
     1861 ccggtacggc gaggcccgtg agcagcatcc cggcgacgac gttgctgtga aagatgtcca
     1921 ggagcgcctg ctcgtcgagg tcggtgatgg gcccgcggta catgttgccg gcgttgctga
     1981 tcagcgcgtc gagccccccg ccgtggcgga ccgcttcgtc gatcatgcgc tgccggtccg
     2041 ctgcgctggt cacgtcgccg acgaccatgg cggctgcgtc gccgatggcc tccttcaccg
     2101 ccgcgagccg gtcctcccgg cgggcggaga tggtgaccct ggccccccgc tcggcgaagt
     2161 aagccgccgc accggcgccg agcccggagc tggcgccggt gacgaggatc gacatgccgg
     2221 cgatgggcct cactcgtcct ctcccagcag ccaggccacc gcctggcgga tgaaccgggc
     2281 atgatgaggg tcgccaaggg atcgtccgtc gtggccgagc gccgagtaga cgaccctgcc
     2341 cgcggcggcc tcgtgcgccc agctgatggg gtgccagtcg ccgtcgggaa cccggccctc
     2401 gagcagtacc ttgctttcgt tgccgatggc caggtggtgg tagatctcgt cgtggagtcc
     2461 gaagtcggtg acgccccgcg tcgccggatg atcggccacc gcgcgcacct ccacgtttcc
     2521 ctcgggcgga tgaaacgtct cgccccagcg ccaggagccg ccgagcagcc tcggaaactc
     2581 gtaccaggtg tcgaagcaga tgcttgcggt gtgcagtccg agcatcccgc cgccggcttc
     2641 cacgaagttc tctatggcct gggcatcttc ctcgggcagc tcgaatgccc actgcttgcg
     2701 gaagggcacg tatttctcgt cgttgagcat gcgccagcgc agggcgtaga tggtgaagag
     2761 gtcggcggat tcgagttccg ccaccgcctc cgagacgtcg ctggtaatga cgctctcgat
     2821 gccgagagat tcgaggagtc gggcgagcac gggtgccgcc tcctcgaacg gatgatagat
     2881 gccgccaatg agcatcacgt tgcgcatcag tccctcggca ggtgcttgag gagggtgtgg
     2941 gtgacgccgc cgtccacgac cagctcgtgg ccgttgacgt acgcgccggc ctccgagtcg
     3001 agaaaggcga ccgcgttggc cacgtcctct gcggtgccca gtcggcccaa cggaaccgcg
     3061 cgggcgcggg tctcccgtac cttcgggtcc gcatagaagg gtgccgacat gccggcatcg
     3121 atgaagccgg gacagaccga attggcgcga atgccccgct cggcgagctc cacggccagc
     3181 tggcgcatca gctggcggac ccctgccttg gccgcgggat aagctccaga gccgggggcc
     3241 ggcgtccggt cgttgatgga ggtgatgctg atgatgtgat ccccgccgct catccgttgg
     3301 gcgcactgcc ggctggccac gtagcagccg atcaggttga cggatatcac cttgacgaag
     3361 tcggccacgc tttcctcgac caccggcccg aagtgcacga tgccggcgtt gttcaccaga
     3421 aggttgggcg gctcgccgaa ggcgtccagc gcccggtcga tgctgtcggc gtcggtcacg
     3481 tcggcggtca gcggcagcgc atcgggcagc gcggccgcca cctccgcggc cgccgtgccg
     3541 tccatgtcga gcacgccgac ccggtatccg gcgcttccca gccgggtggc aatggcggcg
     3601 ccgatgccgt gggcagcgcc ggtcaccagc gctcggcgca cgctcatgtt tgccaccgca
     3661 cgcggacggg aaagcggccc caattgggac gcgaggtgtc ttcgtcgggc accggcaggt
     3721 cggcaggatc gaacatggcg tctagctcgg atagaaagtg atgaaccagg agcgtcaccg
     3781 tgccgtactg atggctgccg gcttcggtat cggaatcttg cggcaggccg gcgtctatgt
     3841 ctcggccgag gcagttgtgc acgccgatcc cgaaggtcag accgaagggg ttggcgcgcc
     3901 ggtccagcac cgcgcgcccg ggctggaacg cgtccgcctc gggcccgaaa acctccgggt
     3961 cccgattggc cgcggcgatg tccaccacca ggtgatcctc cggctccacg tgcaggcccc
     4021 agctgaaatc gatgggctcg agcgcccggc gccaggccac ggggctggca ggatgaagcc
     4081 gtagcgcctc gtgcacggcc cgctgggtgg cgagcgcatc gccggccagg gccgccctgc
     4141 gggcgaggtc gctgccgagc caggtcagca cctcccagag cgcgtgggcg aaggcgttgg
     4201 acgtgctgtg ggcgccggcc tgaaggtaga aggcgatctc ccgcgcgaga ccgtcgtcgt
     4261 cgaggccgcc ccggtccgtc agcagcaccg tgagcatgtc gcgaggcagg gattcctcgg
     4321 gtatctcgct cccacgccac gccgagatta gccgctctcg ccgcgccatg gacggctcca
     4381 ggaagcggcc gcggtactcg gccagcgctt cgcgtacctc tgccctgacc tgatcgtggg
     4441 cgcgcgtgga atgcaccagg gtcgctccct cgccgaattt cttcgtcaac cgctgcaacg
     4501 cggccgtggc ttccgggtcc tcggaccggt cgatgccggc gaaatcggct gtcaggttca
     4561 tggatacggc gtggccgaac gccacgacgt cgatgctggg ctggccccgc agcggctcca
     4621 gggcgtcgcg cagcgctttt ggaaaaacct cgcgctcgta gtgacgggcg tagtctctgc
     4681 ggaagaccct cag
//
LOCUS       DBNHOX010000501         4840 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1133438, whole genome shotgun sequence.
ACCESSION   DBNHOX010000501 DBNHOX010000000
VERSION     DBNHOX010000501.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1133438"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="AC16UZ_10980"
     CDS             complement(<1..765)
                     /locus_tag="AC16UZ_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007249738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR6247250.1"
                     /translation="MLSTPTEANIAAICESLGIEADAAEVAVYRDLVEGSIAGCALVD
                     EMPDDMADWTGGERTWERPAAADNPFNAWYVKTSVAGAPDGPLVGRTLAIKDNVLVGG
                     VPLMNGTSILEGYVPPVDATIVTRILEAGGTIAGKSVCEAYCFSGGSHTASSGFVTNP
                     HDASRAAGGSSSGSGALVAGGVVDMAIGCDQGGSIRMPASFCGVVGMKPTHGLVPYTG
                     ILGMGPAIDHVGPMTATVRDNALLLEVIAGPDGLDSR"
     gene            complement(810..1574)
                     /locus_tag="AC16UZ_10985"
     CDS             complement(810..1574)
                     /locus_tag="AC16UZ_10985"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6247251.1"
                     /translation="MDYLTVDEAKRATGLRLVLTQGVPGPWGESAKALFDLKGVAYHP
                     VAQKGGAANEELLAWTGHRNAPIAILDDEPPRASWLEILFLAERLGESPRLLPEDIDQ
                     RMLVIGLSSEICSPGGFAWQSRMFLLQGMAKAAGDQPENFPMLKDYSYSQEAADRAVA
                     SVKPILDRLGRQLDMQAAAGSDYLVGGELTAVDVYWAFFSQLLVPLPEAICPMPGFLR
                     KSYGMLGTQLEEPAPASLLAHRDRILERHIALPLTF"
     gene            1702..2832
                     /locus_tag="AC16UZ_10990"
     CDS             1702..2832
                     /locus_tag="AC16UZ_10990"
                     /inference="COORDINATES: protein motif:HMM:NF015368.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003847 -
                     1-alkyl-2-acetylglycerophosphocholine esterase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6247252.1"
                     /translation="MHRDLMTSTTFRHLTHETVAAGVTGQEIEAAIDELFSRERGSCL
                     NAPLATHGGPFPVLIASPGDAGSRHNLESVCEHLASHGYAVIVPEHTGSSPFAQLGRD
                     PWLDETGPSAKQAEHVRLVREITDSRGAYGARAGFGQTYSPLRPHREQGTAERQLNDA
                     LHERVRDLDTALNWLAAANARGPWAGRLRADRAGLLGRSFGAATALRAACADERLACT
                     FAVAAPAWRNAPFLELRKPTFLLSGAEDDLLIGMASSQAGAKPTPENPHPALFEAFIR
                     SRAPAMWGLLANADHDAFTCAARYWWPGLKPGQARRALAPDETFELIEPQRAQAVQQL
                     TARAFFDCFLRMDATALARLDPATLAAAGLTFDTRNLEQVAR"
     gene            2829..3014
                     /locus_tag="AC16UZ_10995"
     CDS             2829..3014
                     /locus_tag="AC16UZ_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247253.1"
                     /translation="MTLAEITALISGGGHVEVELHAVDPMIYVAYLVSGNRRQPVTGR
                     ERVSLQAEALRSFQPRR"
     gene            3050..3526
                     /locus_tag="AC16UZ_11000"
     CDS             3050..3526
                     /locus_tag="AC16UZ_11000"
                     /inference="COORDINATES: protein motif:HMM:NF019473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HIR6247254.1"
                     /translation="MIDLSMAGNEDEALALALLKAMEDGDLAAKIEELCTDDFVWANS
                     GLPTLSGKTAVRAHMEAGGFSRQIPILAAMTSFSADVLHIASRAGVVFTERVDHHWDA
                     DGRDLMTPHIAGVMEIRDGRIAALRDFYDVACYAQTPTAPNPDHAMAGTNDKGELT"
     gene            3523..4068
                     /locus_tag="AC16UZ_11005"
     CDS             3523..4068
                     /locus_tag="AC16UZ_11005"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6247255.1"
                     /translation="MKRTVDAEHLIKPAKFAHFVLRVSDIEASIDWYATVLGMEMVHR
                     ADMLAFMTYDEEHHRLALVQTPVEAATPGAPGLDHVAYTLGSLGDLMATYKRLKAADI
                     LPVWPINHGLTTSLYYADPDGNRVEFQVENLDSVEDLQGFMRSDAFAANPIGVGFDPE
                     KLLQRYENGDPLEELLQQGSA"
     gene            complement(4133..>4840)
                     /locus_tag="AC16UZ_11010"
     CDS             complement(4133..>4840)
                     /locus_tag="AC16UZ_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012435.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR6247256.1"
                     /translation="KLNAATVLIAVGQAFFSIGVALAGMMTFGAYLPRDVSIGQSVLV
                     IVFADTLVALTAGLVVFPAVFHFGLDPAGGPGLIFQTLPVAFGQMPGGRLVSILFFLL
                     LSVAAVTSMVGLLEPLTSWLEEHRGLSRHGSAVLVLGSVAALSVLSILSYNVLTGFRV
                     FGRDLNGVFDYLSNQIMLPLGGFLIALFVGWFVSKATSRDELDIPDSRYFQTWHALIR
                     YVVPPAVLAILVFGVMS"
BASE COUNT          892 a         1667 c         1557 g          724 t
ORIGIN      
        1 ccgactgtcc aggccgtccg ggccagcgat gacctcgagc agaagggcgt tgtcacgcac
       61 ggtagcggtc atgggcccga catgatcgat ggccgggccc atgccgagga tgcccgtgta
      121 cggcaccagc ccgtgggtgg gcttcatgcc caccacaccg cagaacgaag cgggcatgcg
      181 gatcgacccc ccctggtcgc agccgatggc catgtccacg acgccgcctg caacgagagc
      241 gccgctgccc gacgaggaac cgccagcggc tcggctcgca tcgtgggggt tcgtgacgaa
      301 tcccgagctg gcagtgtggc tgccgccgga aaagcagtag gcctcgcaga ccgacttgcc
      361 ggcaatggtc ccacccgcct cgagaatccg ggtcacgatg gtggcatcga ccggcgggac
      421 gtagccttcg aggatgctgg tgccgttcat cagcggcacg ccgcccacga gcacattgtc
      481 cttgatggcc agggttcgcc cgaccagcgg gccgtcgggc gcgccggcga cgctggtctt
      541 cacgtaccag gcgttgaaag ggttgtcggc agcggccggg cgctcccacg tgcgttcacc
      601 cccggtccaa tccgccatgt cgtccggcat ctcgtccacc agggcacacc ccgcgatgga
      661 accctccacc aggtcacggt aaacggcgac ctccgcggca tcggcctcga tgccaagaga
      721 ttcacagatg gctgcgatgt ttgcttcggt tggcgtgctg agcatggtct tctccctccc
      781 agagcggcga agccgcgtga aaccaatcgt caaaaagtca gcggcagcgc gatgtgccgc
      841 tcgagaatcc ggtcgcgatg ggccagcagg ctggccggcg ccggctcctc gagctgggtt
      901 cccagcatcc cgtaagattt ccggagaaag cccggcatgg gacagatggc ttccggcagc
      961 ggcacgagca gctgtgagaa gaacgcccag taaacgtcca ccgccgtcag ctcgcctcct
     1021 accaggtagt cgctgccggc agcggcctgc atatccagct gccgcccaag ccggtcgagt
     1081 atgggcttca cgctcgcgac tgcccggtcg gccgcttcct ggctgtagct gtaatccttg
     1141 agcatgggaa agttttccgg ctgatcgccg gccgccttgg ccatcccctg gagcagaaac
     1201 atgcgggact gccaggcaaa gccgccggga gagcagatct cgctggaaag cccgatgacg
     1261 agcatgcgct ggtcgatgtc ctcgggcaga agccggggcg actcgccgag ccgctcggcg
     1321 agaaagagga tctcgagcca gctcgcccgc ggcggctcgt cgtcgagaat ggcgatgggc
     1381 gcgtttcgat ggcccgtcca ggccagcaac tcctcgtttg ccgccccgcc cttctgcgcc
     1441 acgggatggt aggcaacgcc cttcaggtcg aacagcgcct tggccgactc accccagggg
     1501 ccaggcacgc cctgggtgag cactagccgc aaacccgttg cccgttttgc ctcgtcaacc
     1561 gtcagataat ccatctcccc tctaccccag cgatgtcgat cccaagttat accccgaaaa
     1621 cgtcaggcat ctcgaccgcg gcgcgacccg gtctgcgcga ccagggccac ctacgccgac
     1681 tacgtgttcg gggatgccag cgtgcaccgc gacctcatga ccagcacgac gttccggcac
     1741 ctgactcacg aaaccgtcgc ggccggcgtg accggccagg agatcgaagc cgcaatcgac
     1801 gaactctttt cgcgcgagcg cggcagctgc ctgaatgccc ctttggcaac acacggcgga
     1861 ccctttcccg tgttgattgc atctcccgga gacgccggca gccggcacaa cctggagagc
     1921 gtctgcgagc atctggccag ccacgggtac gcggtgatcg tgcccgagca caccggcagc
     1981 agcccgtttg ctcagctcgg acgggatccc tggctggacg agaccggccc ctctgcaaaa
     2041 caggcagagc acgtccgcct ggtgagggag atcacggaca gccgcggagc ctacggagca
     2101 agggccggct tcgggcagac ctactcaccg ctgcgaccgc acagggagca aggcacggca
     2161 gagcgacagc tgaacgatgc gctccacgag cgggtgcgcg acctggacac cgccttgaac
     2221 tggctggctg ccgccaacgc tcggggaccc tgggcaggac gcctgcgcgc cgaccgcgcc
     2281 ggtctcctcg gccgctcatt cggtgctgcc acggctctgc gcgccgcctg cgccgatgag
     2341 cgcctggcct gcacgttcgc cgtggcggcg cccgcctggc gcaacgcccc gtttctcgaa
     2401 ttgcgcaagc cgacgttcct gttgagcggc gccgaagacg atctgctgat cggcatggcc
     2461 agcagccagg ccggggcgaa gccgaccccg gagaatcctc accctgcgct ctttgaagcc
     2521 ttcatccgga gccgtgcgcc agccatgtgg ggtctgctcg cgaacgccga ccacgacgcg
     2581 ttcacctgcg cagcccggta ctggtggccg gggctgaagc cgggccaggc gcgacgcgcc
     2641 ctcgctccgg atgagacctt cgagctgatc gagccgcagc gggcccaggc cgttcagcag
     2701 ctcaccgcgc gggccttctt cgactgcttc ctgcggatgg acgcaacggc gctcgccagg
     2761 ctggatccgg cgacgctggc ggcggctggg ctgaccttcg acacccgcaa cctcgagcag
     2821 gttgcccgat gacgctcgcc gagatcaccg ccctcatcag cggcggcggc cacgtcgagg
     2881 tcgagctcca cgccgtcgac ccgatgattt acgtggctta ccttgtttca ggcaaccggc
     2941 ggcagcccgt cacggggcgc gagcgggtgt cgctgcaagc cgaggccttg cgctcgttcc
     3001 agccccggcg ttagggttcg ggctgagcgc ttctgggaga cagcaccgga tgatcgacct
     3061 ctccatggcg ggcaacgaag acgaggcgct cgcgctggcg ctgctcaagg cgatggagga
     3121 tggtgacctt gccgccaaga tcgaggagct gtgcaccgac gacttcgtct gggccaacag
     3181 cgggctgccg acgctgagcg gcaagaccgc cgtgcgcgct cacatggaag ccggtggctt
     3241 ttcccggcag atacccatcc tggctgctat gacgtctttc agcgccgacg tgctgcacat
     3301 tgccagccgg gccggggtgg tgttcaccga gcgcgtcgat caccattggg acgccgacgg
     3361 ccgggacctg atgacaccgc acatcgcggg cgtcatggaa atccgcgacg gcaggatcgc
     3421 cgccctgcgt gacttctacg acgttgcctg ctacgcgcag acgcccacgg ccccgaatcc
     3481 cgaccatgcc atggcaggca ccaacgacaa aggagagctg acatgaagag aaccgtcgac
     3541 gcagagcatc tgatcaagcc ggccaagttc gcgcacttcg tgctgcgcgt atcggacatc
     3601 gaagcatcca tcgactggta cgccaccgtg ctcggcatgg agatggtgca ccgggccgac
     3661 atgctggcgt tcatgaccta cgacgaggag catcaccgcc tggccctcgt gcaaacgccc
     3721 gtcgaggctg ccacccccgg tgctccggga ctcgaccacg tagcctacac gctgggctcc
     3781 ctgggcgatc tcatggctac ctacaagcgc ttgaaggcag cggacatcct gccggtgtgg
     3841 ccgatcaacc acgggctgac gacctccctg tactacgcgg accccgacgg caaccgagtc
     3901 gagttccagg tcgagaacct cgactccgtg gaggacctgc agggcttcat gcgttcagat
     3961 gcctttgccg cgaaccccat cggcgtgggc ttcgaccccg agaagttgct gcagcgttac
     4021 gaaaacggcg atccgctcga ggaactcctc cagcagggct cggcctgacg caggaacgcg
     4081 ccgggccaac agggtcttcg atcggcccca cgccgcaggc cggcgcgcgt cgtcagctca
     4141 tcacgccaaa aacgagaatt gccagcaccg ccggaggcac cacgtagcga atcaacgcgt
     4201 gccacgtctg gaagtaccgg gagtcgggaa tgtccagctc gtcccgcgat gtcgccttgc
     4261 tgacaaacca gccaacgaac agcgcgatga gaaagccgcc gagcggaagc atgatctggt
     4321 tcgacaggta gtcgaacacg ccgttgaggt cccgaccgaa aacgcggaat ccggtgagga
     4381 cgttgtagga gagaatgctc agcacgctca gggccgccac cgaacccagc acgagcaccg
     4441 cgctgccgtg ccggctgagg ccccggtgct cctcgagcca ggacgtgagc ggctccaaga
     4501 gccctaccat ggaggtaacg gccgcaacgg agagcagcag aaagaagaga atgctgacca
     4561 gccgcccacc cggcatctgg ccgaaagcca ccggcagcgt ctgaaagatg aggccggggc
     4621 cgccggccgg gtcgagaccg aagtgaaaca ccgcgggaaa gaccaccagc cccgccgtca
     4681 gcgccacgag ggtgtcggcg aacacgatca ccaggacgct ttgaccgatg gaaacgtcgc
     4741 gcggcaggta ggcgccgaac gtcatcattc ccgccagcgc tacgccgatg gagaagaagg
     4801 cctggcccac agctatcagg accgtggccg cgttcagctt
//
LOCUS       DBNHOX010000502         3194 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_746987, whole genome shotgun sequence.
ACCESSION   DBNHOX010000502 DBNHOX010000000
VERSION     DBNHOX010000502.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_746987"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="AC16UZ_11015"
     CDS             complement(<1..828)
                     /locus_tag="AC16UZ_11015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6247257.1"
                     /translation="MKVDGGVGWELDLVGAQAQELEEMGYSGILSAETSHDPFFPLLV
                     AAQNTERVDLMTSIAVAFARSPMTLANIGHDLNAVSKGRFVLGLGSQIRAHIMKRFSM
                     PWSHPAARMREFILAMRAIWSNWHEGEPLAFTGKFYTHTLMTPFFTPTDTEYGPPRVF
                     LAAVGPMMTEVAGEVADGVIVHAFTTEKYLRETTLPALERGFAKAGKTRADFEISYPV
                     FVVTGENEEELAQAKVATARQIAFYGSTPAYKPVLESIGVGEMQGELNAMSKQGRWEE
                     "
     gene            complement(847..1524)
                     /locus_tag="AC16UZ_11020"
     CDS             complement(847..1524)
                     /locus_tag="AC16UZ_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6247258.1"
                     /translation="MKRLYLIRHGQTEWNVAKRMQGRLDSPLTSAGRIQAAVHGRLLS
                     EIASLEQLVVSPSGRTRETACILNSHVGAPMRHDEALMERDCGEWSGLTIEEIEQHFP
                     EAWEARREDPFYHRPPGGENHEDLVQRAGPLLAEFLAGDVTEVGLVTHGVMSRAIVTH
                     LLELTPVEAVGIRHPNDLVYRLDICATGVQVSHFLAGDGPRAGLLRQNENETISGPAR
                     AGERRDR"
     gene            complement(1558..2400)
                     /locus_tag="AC16UZ_11025"
     CDS             complement(1558..2400)
                     /locus_tag="AC16UZ_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6247259.1"
                     /translation="MEYQKIIVDEPRPMVRRITLNRPEKRNPLSNELRSEMFHALESA
                     DQDDGVRVTIIRGAGPCFSAGYDLKSDTSQNPPFYTAGGLGHWPRHVVEGFFRMWDMA
                     KPIIAQVHGYCLAGGTELATACDLVYVAEDAKIGYPVVRTMSPPDNQFYPWIVGLRRA
                     MELMLTGDHMSGVEAAESGFANRAFPAEELEERVLAIAERVALTPTDLQQFNKRAVHR
                     QMDAMGIRAGIRAGTEMQQLAFFTDTTRAYFKEMSKGLTHALSKRDEAFGDYRTSASG
                     KAED"
     gene            complement(2442..>3194)
                     /locus_tag="AC16UZ_11030"
     CDS             complement(2442..>3194)
                     /locus_tag="AC16UZ_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6247260.1"
                     /translation="EQVLPGRFVMGLGSSSQTIMGQWNGIPLDRPLTRVRETAILVRS
                     MLTGEKSNFDGETLTSHGYRQPPLDHPPPIYLAALRPKMIEMAAEVGDGVIFNLWPKS
                     VLPKMMEHVRIGAERAGKNWEDVEVVNRAMVLCTNDKQAGRNLFRAAFAPYYATPVYN
                     AFLAWAGHADAAGAITEGWAAKDRAMTTGALSDELIDEIAIIGDEDEIRARIQADADG
                     GVHTHIVAPLAASPEDVERTFAAFTADGFSLK"
BASE COUNT          538 a         1058 c          997 g          601 t
ORIGIN      
        1 ctcttcccac cggccctgct tggacatggc gttcagctcg ccctgcatct cgccgacgcc
       61 gatgctttcc agcaccggct tgtaggcggg cgtggaccca tagaaggcga tctgacgcgc
      121 cgtggccacc ttcgcttgcg ccagctcttc ctcgttctcc ccggtcacga cgaataccgg
      181 atagctgatc tcgaagtcgg cgcgcgtctt gcccgccttg gcaaaacccc gctccagagc
      241 cggcagcgtg gtttcgcgca ggtacttctc cgtggtgaac gcgtgaacta tcacgccgtc
      301 ggcgacctcg ccggcgacct cggtcatcat gggcccgacg gcggcgagaa agacgcgcgg
      361 cggtccgtat tcggtgtccg tgggggtgaa gaacggcgtc atgagcgtgt gggtgtagaa
      421 cttgcccgtg aaggccaggg gctctccctc gtgccagttc gaccagatgg ccctcattgc
      481 caggatgaat tcgcgcatgc gcgccgccgg gtgagaccag ggcatgctga atcgcttcat
      541 gatgtgcgcc cggatctgcg agccgaggcc cagcacgaaa cggcctttgg aaacggcgtt
      601 cagatcgtgg ccgatgttgg ccagagtcat gggcgatcgg gcaaaagcca ccgcaatcga
      661 ggtcatcagg tcgacccgct ccgtgttctg cgccgccacg agcagcggga agaacgggtc
      721 gtggctggtt tcagccgaca ggattcccga atagcccatc tcctcgagct cctgggcctg
      781 agcgcccacc agatcgagtt cccaacccac accgccgtcg actttcatcg tcttccccca
      841 gtttttttat cggtcccgcc gctctccggc ccgagcgggg ccggagattg tttcattctc
      901 gttttggcga agcaatcccg cccgcggacc gtctcccgcg agaaagtgac tgacctggac
      961 accggttgcg cagatatcca gccgatagac cagatcgttg gggtgacgga tcccgaccgc
     1021 ttccacgggc gtcagctcga gcagatgggt cacgatcgcc cgggacatga cgccgtgggt
     1081 caccaggccc acctcggtaa cgtctccggc cagaaactcc gccagcaacg ggcccgcccg
     1141 ctgcaccaga tcctcgtgat tctcgccgcc aggtggccgg tgataaaagg gatcctcccg
     1201 ccgggcctcc caggcctccg gaaagtgttg ctcgatctct tcgatggtga gccccgacca
     1261 ctcaccgcag tcgcgttcca tgagggcttc gtcatgacgc atcggcgccc caacgtggga
     1321 gttcagtatg caggcggttt cgcgcgtccg tccggacggg gacaccacca gctgctcgag
     1381 actggcgatc tcgctcagca gacgaccatg aacggccgcc tggatccgac cggccgaggt
     1441 caggggcgaa tcgaggcgtc cctgcatgcg cttggccaca ttccactcag tctggccgtg
     1501 acggatcaga tagagccgct tcatgattcc cgttggagat cccggcaccg gagcccgtca
     1561 gtcctcggcc ttgccggacg cggaggtccg gtaatctccg aaagcctcat cgcgtttcga
     1621 caaagcgtgg gtcagcccct tgctcatctc tttgaagtac gcacgcgtgg tgtccgtgaa
     1681 gaaggcgagc tgctgcatct ccgtgccggc acgaatgccg gcccggattc ccatggcgtc
     1741 catctgccga tgcacggcgc gtttgttgaa ttgctgcaga tccgtgggcg tgagggcgac
     1801 gcgctcggct atcgccagaa cccgctcctc cagctcctcg gcgggaaagg cacggttggc
     1861 aaagccgctt tctgccgctt cgacccccga catgtgatca ccggtgagca tgagctccat
     1921 ggcccgcctg aggccgacga tccaggggta gaactgattg tccgggggcg acatggtgcg
     1981 cacgaccgga tagccgatct tggcatcctc cgccacgtaa acaaggtcgc aggccgtggc
     2041 aagctcggta ccgccggcca ggcagtatcc gtgcacctgg gcaatgatcg gcttggccat
     2101 gtcccacatg cggaagaatc cttccaccac gtgccgcggc cagtgaccaa ggccgccggc
     2161 cgtgtagaac ggcgggttct ggctggtatc gctcttcaga tcgtagcccg ccgagaagca
     2221 cggaccggcc ccgcgtatga tggtgacccg aaccccatcg tcctgatcgg cgctttccag
     2281 cgcgtggaac atctccgagc gcagctcgtt ggagagcggg ttgcgcttct cgggccggtt
     2341 gagcgttatg cggcggacca tgggccgcgg ctcgtcgacg atgatcttct ggtattccat
     2401 gatctcctca ctgtcttcgg atcgacggac ccggagggtg cctatttcaa actgaacccg
     2461 tctgccgtga acgcggcgaa cgttcgctcc acgtcttcgg gcgaggcggc caggggagcg
     2521 acgatgtggg tgtggacgcc gccgtccgca tccgcctgta tgcgcgcgcg gatctcatcc
     2581 tcgtcgccga tgatggcgat ctcgtcgatg agctcatcgc tcagggcgcc ggtcgtcatc
     2641 gcccggtctt tcgccgccca gccctcggta atggcgccgg cggcatcggc atgacccgcc
     2701 caggcaagaa acgcgttgta aacgggggtg gcgtaatagg gcgcaaaggc agcccggaac
     2761 aggttccggc ccgcctgctt gtcgttcgtg cacagaacca ttgcacggtt gacgacctct
     2821 acgtcttccc agttctttcc ggcgcgctcg gcgccgatgc gaacgtgctc catcatcttc
     2881 ggcagcacgc tcttaggcca cagattgaag atcacaccgt ccccgacctc ggccgccatc
     2941 tcgatcatct tcgggcgcag ggcggccaga tagatgggcg gcggatggtc caggggtggc
     3001 tggcggtaac cgtggctcgt caacgtctcg ccgtcgaagt tgctcttctc gccggtcagc
     3061 atggaacgca ccaggattgc cgtctcccgg acccgggtca ggggccgatc cagcggaatc
     3121 ccgttccatt gacccatgat ggtctggctc gaggagccca ggcccatgac gaaacgtccg
     3181 ggcaggacct gctc
//
LOCUS       DBNHOX010000503         6374 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1775047, whole genome shotgun sequence.
ACCESSION   DBNHOX010000503 DBNHOX010000000
VERSION     DBNHOX010000503.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1775047"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..126
                     /locus_tag="AC16UZ_11035"
     CDS             31..126
                     /locus_tag="AC16UZ_11035"
                     /inference="COORDINATES: protein motif:HMM:NF014009.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247261.1"
                     /translation="MLLGTWQGVFLWEHRHHHDRREIIVSVLDGS"
     gene            complement(104..508)
                     /locus_tag="AC16UZ_11040"
     CDS             complement(104..508)
                     /locus_tag="AC16UZ_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247262.1"
                     /translation="MRKEDLQAAFGPVLDEVAERSQVADAFLDKDLYRIYIATLWANV
                     VLDPSDVGIGEADLETVHDLVNEQIGDVLGSGTDIRECFRFVNSKAGERAMGAAKLTQ
                     RHKELLLYFSSMILDPDGHRRWMENVRNRLER"
     gene            729..1874
                     /locus_tag="AC16UZ_11045"
     CDS             729..1874
                     /locus_tag="AC16UZ_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020296375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6247263.1"
                     /translation="MLQFDSMELPEETVALRSEVRSFLDSEHSHLTRRNSDFGSGHDP
                     EFSRKLGAKGWIGMAWPKAYGGGERTFFERYVVTEELLSAGAPVGAHWIADRQSGPLL
                     LRFGTEAQREKYIPPITRGESYFSIGMSEPNSGSDLASVRTTASKVADGWVVNGTKLW
                     STDAHRNNYMIALVRTEPPSENRHAGLSQVIIDLHDDGAQIRPIKNIAGHEDFNEVVF
                     EDVLIPEDRLVGEPGNGWEQVTSELAYERAGPERFLSSFRVFVELVRAVGKDPLPHEA
                     DAIGRIASHLMALRRMSISVAGMLEAGKSPQVEAALVKELGNNFEKLLPEIARLAAPD
                     GLSGDEERLRSFRSTFTDTLLGSPSFTIRGGTREILRGVIARGLGLR"
     gene            1874..2824
                     /locus_tag="AC16UZ_11050"
     CDS             1874..2824
                     /locus_tag="AC16UZ_11050"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6247264.1"
                     /translation="MSTRQMLTDSAERIFADHCTKALLDGAQEGEFPGDLWAVLKENG
                     FAELATADSGFELGDAFAVLKVAGRHAAPLPLAEIMLGNRWLGSADRLVSVGLVDGDV
                     VRDVPWGRRADAVIALDPSGGPPSVLEVTQVDSTGSNLAGEARDVLALTAALPLGSNE
                     PGLELLALARVAQMAGALERILELTLRYANEREQFGRPIARFQAIQHNLAVMAAEVAA
                     AVRIADAAVDALGGERFEFEVAAAKSRVGEAAGVCAESAHQIHGAMGFTHEHQLHHFT
                     RRVWAWREEFGNEIYWRRRLGDHIAGLGADAVWDFLATRG"
     gene            2817..3806
                     /locus_tag="AC16UZ_11055"
     CDS             2817..3806
                     /locus_tag="AC16UZ_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009045556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-thiouracil desulfurase family protein"
                     /protein_id="HIR6247265.1"
                     /translation="MADALPERPVPPLPIAISECLLGSKVRYDGSSSRSSFPHTLLDG
                     LFTYRGICPEVGIGMGTPREPIRLVGKPDDARVVGVKDPTMDVTERLIEFGRETAASL
                     TDVCGYVLMKNSPSCGLFRVKVYPVTDGEVTGAPDPRGRGAHAAAITAALPDLPAEEN
                     GRLHDPVLRENFVTRVFTYGHWQRCFGSADDLTAAKLVGFHSRYKYLLMAHSVPDYEA
                     AGRLLSDLRGDLPGKAAEYFSLLMRGLSRPATRKGHANVLSHLQGYFKRLLDGPSRQE
                     LEALIHSYRRGEQPLDAPLTLLKHHLRRHPQEYLEYQVYLDPHPGFAGLRRLL"
     gene            3915..5315
                     /locus_tag="AC16UZ_11060"
     CDS             3915..5315
                     /locus_tag="AC16UZ_11060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6247266.1"
                     /translation="MSKDYDVIVVGSGAAGLAAAVAAADAGASVLLVEADKRVGGSSR
                     LSGGHFYAAGTSTQREAGVDDDTADAMFEHYMTLNQWLVEPSVVRRYCDLSAPTFEWL
                     KGQGVSFSPQAVYRSGVGSVARGHPPEGSGEAVVNILDARRSSLGVDVVLDARVDGLL
                     LDDAGRVHGIRIGDDEATCGAVVMATGGFGANPEMLAEYYPAAAANGDWSWYIGTEMA
                     RGDGIRLGQDAGAAIDGHNRGLLLVTPGFSRDLEVLLPGWLVLINEQGRRFTDETAPY
                     TVLAGLIQHQTGKVYAVFDEAARRGAKRNPASQAYWVDEVLEQKADAGRILRADSLDE
                     LAAAAGIHADGFKGTVARYNADCEKGEDTAFFKSPETGMRAIEEGPFYAAEVRPAIIC
                     WTGTGLRIDAEARVLRTDEHPIPGLFAAGELVGSLHGDRYIGGGGSFGPCIVFGKLAG
                     ENAASYTLTQAWAQDR"
     gene            complement(5287..>6374)
                     /locus_tag="AC16UZ_11065"
     CDS             complement(5287..>6374)
                     /locus_tag="AC16UZ_11065"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6247267.1"
                     /translation="IVITLASLATGLALAIGGLDLLGGAIAIGTLVAFMAYTRHFFDP
                     IEQLGHWFAEMQMAQASAERILSLIETDPAVKDSPDVIEALQRQADAPPRRNYAPDGG
                     AANIRQIELRNVAFAYDPAKPVLKDINLTINRGETVAVVGPTGGGKSTLVNVICRFYE
                     PTAGQVLIDGVDYRNRSLHWLQANLGMVLQNAHVFSGSIMENIRYGRLDASDEEVREA
                     ARLAGADEFVRAVEKGYDTEAGEGGNRLSAGQKQLVSFARAILADPQILVMDEATSSV
                     DTHTEQRIQRGMANVLAGRIAIVIAHRLSTIRNADRIIVVEEGRITESGTHAELMGRR
                     GHYFELYKRQGLQESSATLSDLEPRPA"
BASE COUNT         1022 a         2028 c         2187 g         1137 t
ORIGIN      
        1 agcttgtcaa ttccggtcca ggatggtgcc gtgctgttgg ggacctggca gggcgtgttt
       61 ctctgggagc accggcacca ccatgaccgg cgcgagatca tcgtcagcgt tctagacggt
      121 tcctgacgtt ctccatccag cggcggtgac cgtccggatc gagaatcatg gacgagaagt
      181 acagcagcag ctccttgtgg cgctgggtca gcttggccgc gcccatggcg cgctctccgg
      241 ccttgctgtt gacgaaccgg aaacactcgc ggatgtcggt gccgctgccg agcacgtcgc
      301 cgatctgctc gttcaccaga tcatggacgg tttccaggtc tgcctcgccg atgccgacgt
      361 cggaagggtc gaggacgacg tttgcccata gcgtggcgat gtagatcctg tagagatcct
      421 tgtccaggaa agcgtcggct acctgggaac gctcggcgac ctcatcgagc accggtccga
      481 aggcggcctg cagatcctct ttcctcatgc tgccggcggt cgccgccggt tgagcgcgcg
      541 ttcgcccggg gtaggtctat tcgagctctg ctctcgtttg gggatcgacg tcatcggatt
      601 tttcagctgc ggaatcgatg tcgctagtgt aggcaacgcg aaattgcccg acaacgaggc
      661 gagcggtttg caatgaacct gggctgtcag taatttagcg acgccttatc tgcattggac
      721 gattcccggt gttgcaattc gactccatgg agctgcccga ggaaacggtg gcgctgcgca
      781 gcgaggtgcg ctcgtttctc gacagcgagc attcgcacct tacgcgccga aactcggatt
      841 tcggttccgg tcacgaccct gaattttccc ggaagctggg tgcgaagggt tggatcggca
      901 tggcctggcc caaggcctac ggcggcgggg agagaacgtt tttcgagcgc tacgtggtta
      961 ccgaggaact cttgagcgcg ggcgccccgg tgggcgcgca ctggatcgcg gaccgtcaga
     1021 gcggaccgct gctgctgcgc ttcggaaccg aggcccagcg tgagaagtac atcccgccga
     1081 tcacgcgggg cgaaagctac ttctccatcg gcatgagcga gccgaactcg ggatctgacc
     1141 tcgcctccgt gcggaccacg gccagcaagg tcgcggatgg ttgggtcgtc aacgggacga
     1201 agctgtggtc cacggacgcg catcgcaaca actacatgat cgcgctggtt cgcaccgagc
     1261 ctccctctga gaaccgtcat gccggtctct cccaggtgat catcgatctg cacgacgacg
     1321 gggcgcagat ccgtccgatc aagaacatcg ccgggcacga ggacttcaac gaggtggtct
     1381 tcgaagacgt tctgattcct gaggaccgtc tggtcggcga gcccggcaac ggctgggagc
     1441 aggtcacgag cgagctcgcc tacgagcggg cgggtccgga acggttcctg tcgagtttcc
     1501 gcgtattcgt ggagctggtc agggccgtgg gtaaagatcc gcttcctcac gaagctgacg
     1561 ccattggccg catcgcttcg cacctcatgg cgctgcggcg catgtccatc tccgtggccg
     1621 gcatgctcga agcgggcaag agcccccagg tggaagctgc tttggtcaag gagctgggca
     1681 acaacttcga gaagcttctt ccggagatcg cgcggctcgc ggctccggac ggtctgagcg
     1741 gcgacgaaga gcggctgcgt tcgttccgct cgacgtttac ggatacgctg ctcgggtcgc
     1801 cctcgttcac catccgcggc gggacgcggg aaattcttcg gggcgtgatt gcccgcgggc
     1861 tggggctgcg ctgatgagca cgcgtcagat gctcacggac agcgcggaaa ggatcttcgc
     1921 cgatcactgc accaaggccc tgctggacgg ggcacaggaa ggagagtttc ccggcgacct
     1981 ctgggccgtg ctcaaggaga acggctttgc cgagcttgcg acggcagaca gcggcttcga
     2041 gctcggagat gctttcgccg tgctcaaggt ggcgggccgt cacgccgcgc cgctgccgct
     2101 ggccgagatc atgctgggca atcggtggct gggcagtgcc gatcgtctgg tgtcggtggg
     2161 gctcgtggac ggggacgtgg tgagagacgt gccctggggc cgccgggcgg acgccgtgat
     2221 cgcgctggac ccctcgggcg gacccccatc cgtgctggag gtgacgcagg tcgactctac
     2281 gggcagcaac ctggccggcg aagctcggga cgtgctcgcc ctgaccgcag ccttgccgct
     2341 tggcagcaac gaacccggcc tcgagctcct ggcccttgcc cgcgtggccc agatggcggg
     2401 ggcgctggaa cggatactcg agcttaccct gcggtatgcc aacgaacggg agcagttcgg
     2461 tcgccccatc gcgcgctttc aggccattca gcacaatctg gctgtcatgg cggccgaggt
     2521 agccgccgcg gtgcgcatcg ccgacgccgc cgtggatgcc ctgggcggtg agcgtttcga
     2581 gttcgaggtt gccgccgcca agtccagggt cggcgaggct gccggcgttt gcgcggaaag
     2641 cgcccatcag attcacggcg ccatgggctt cacccacgag catcagctgc accatttcac
     2701 ccgccgcgtc tgggcctggc gcgaggagtt cggcaacgag atctactggc gccgtcgcct
     2761 gggagaccac atcgccgggc tgggtgccga tgccgtgtgg gacttcctcg ccacgcgtgg
     2821 ctgacgccct gccagagcgg ccggtcccgc ccctgcccat tgccatcagc gagtgcctcc
     2881 tgggcagcaa ggtacgctac gacggcagct cgtcacgatc gtcgttcccg cacacgctgc
     2941 tcgacggtct gttcacgtat cggggaatct gtccggaggt gggcatcggc atgggcacgc
     3001 cgcgggagcc tatcaggctc gttggcaagc cggacgatgc gcgggtcgtg ggggtgaaag
     3061 acccgacgat ggacgtcacc gagcggctca ttgaattcgg tcgggagacg gcggcgtcgc
     3121 tgacggacgt ctgcggttac gttctcatga agaactcgcc cagctgcggc ctgttcaggg
     3181 tcaaggttta ccctgtcacc gacggcgagg tgacgggcgc gcccgatccc cggggccgcg
     3241 gcgctcacgc tgcggcgatt accgccgcgc tgcccgacct gccggcggag gagaacggca
     3301 ggctgcacga tccagtgctg cgggaaaact tcgtgacccg ggttttcacc tacgggcact
     3361 ggcaacgctg tttcggctcg gctgacgatc tcaccgccgc aaagctcgtc ggctttcaca
     3421 gccggtacaa gtacctgctc atggcgcaca gcgtcccgga ctatgaggcg gcgggcaggc
     3481 tcctgagcga tctgcgcggc gatctgcccg gcaaggcggc cgagtatttc tccctgctca
     3541 tgcggggcct gtcccgaccg gcgacccgca aagggcacgc caacgtgctc tcccatctgc
     3601 agggatattt caagcgcctt ctggacgggc ccagccgcca ggagctcgaa gcgctgattc
     3661 acagctaccg ccgtggcgag cagccgctgg acgctccgct caccctgttg aagcatcatc
     3721 tgcgccgtca ccctcaggag tatctggagt atcaggtcta tctggatcct catccgggct
     3781 ttgccggcct gcgtcgattg ctctgagcgt tctgcgccct ggggcccttg ccgttgacca
     3841 accggtggcg cgtcgccctt aacggggtaa actcttcccg acgcgatggt gaacgcagcc
     3901 aggggaggga caccatgagc aaagactacg atgtgatcgt cgtggggagc ggcgcagccg
     3961 gtctcgccgc ggccgtcgct gctgcagacg ccggagccag cgtgcttctg gtcgaggcgg
     4021 acaaacgggt gggcggatcg tcacgtctca gcggcgggca tttctatgcg gcgggcacca
     4081 gcacccagcg ggaggccggg gtcgacgacg acacggccga tgccatgttc gagcactaca
     4141 tgacgctgaa ccagtggctc gtcgagccct cggtggtccg ccgctactgc gatctgtccg
     4201 cgcccacgtt cgagtggctc aagggtcagg gcgtcagctt cagcccccag gccgtctacc
     4261 ggtccggggt gggcagcgtt gcccgtggcc acccgccgga aggatccgga gaggccgtcg
     4321 tcaacatcct cgacgcccgt cgttcgagtc tgggcgtgga cgtggtgctg gacgcgagag
     4381 tggacgggct tctcctcgat gacgccgggc gggtccacgg catcaggatc ggcgacgacg
     4441 aggcgacctg cggggccgtg gtgatggcca ccggggggtt cggtgccaac cctgagatgc
     4501 tcgcggagta ctacccggct gcggcggcca acggcgactg gtcgtggtac atcggaaccg
     4561 agatggcccg gggcgacggc atccggctcg gtcaggacgc cggtgccgcc atcgacggcc
     4621 acaaccgcgg tctgctgctc gtcacgcccg gcttcagccg cgatctggaa gttctgctcc
     4681 cgggatggct ggtcctcatc aacgagcagg gacgccgctt caccgacgag acggcgccct
     4741 acacggtgct tgcggggctc atccagcatc agacaggaaa ggtgtacgcg gtgttcgacg
     4801 aagccgccag gcgcggggcc aaacgcaacc cggcctccca ggcgtactgg gtggacgagg
     4861 ttttagagca gaaggccgat gcggggcgca tccttcgggc ggactccctc gacgagctcg
     4921 ccgctgctgc gggcattcac gcggatggct ttaagggcac cgtggcccgc tacaacgccg
     4981 actgcgagaa gggcgaggac acggcgttct tcaaaagccc ggaaaccggc atgcgcgcca
     5041 tcgaggaggg accgttctac gctgcggaag ttcgtccggc catcatctgc tggacgggca
     5101 cgggcctgcg catcgacgcg gaggcccggg tgttgcgcac cgacgagcac ccgattcccg
     5161 ggctcttcgc cgcaggcgag ctggtgggct cgctgcacgg agaccgctac atcggcggtg
     5221 gcggttcttt cggcccctgc atcgtgttcg gcaagctggc tggtgagaac gccgcctctt
     5281 acacgctcac gcaggcctgg gctcaagatc gctgagggtg gcgctcgact cctgcaggcc
     5341 ctgccgctta tagagctcga aatagtggcc acgtcggccc atgagctcgg cgtgggtgcc
     5401 cgactcggtg atgcggccct cctcgaccac gatgatgcgg tcagcgttgc ggatggtgga
     5461 cagccggtgg gcgatgacga tggcgatgcg tcccgccagc acgttggcca tgccccgctg
     5521 aatccgctgc tcggtgtgcg tatccaccga tgaggtggcc tcgtccatga cgagaatctg
     5581 cgggtccgcc agtatggcgc gggcgaagga gacgagctgc ttctgtcccg ccgaaagacg
     5641 attcccgccc tcaccggctt ccgtgtcgta gcctttctcc acggcgcgga cgaactcgtc
     5701 cgcgccggcc agccgggctg cttcccgcac ctcctcgtcg ctcgcgtcga gccgtccgta
     5761 gcggatgttc tccatgatcg agccgctgaa aacgtgggcg ttctgcagaa ccattccgag
     5821 gttggcctgc agccaatgga ggctgcggtt ccggtagtcg acgccgtcga tgagcacctg
     5881 gcctgcggtg ggctcgtaga aacggcagat cacgttgacc agcgtgctct tgccgccgcc
     5941 ggtcggcccg accacggcca cggtctcgcc gcggttgatg gtgaggttga tgtccttcaa
     6001 caccggtttc gccggatcat aggcaaacgc cacgttgcgc agctcgatct gacggatgtt
     6061 cgccgcccca ccgtcgggcg cgtagttccg gcgggggggt gcatcggctt ggcgctgcag
     6121 agcctcgatc acgtcgggtg agtctttcac cgccgggtct gtctcgatga ggctcagtat
     6181 ccgctccgcc gaggcctggg ccatctgcat ctcggcgaac cagtggccga gctgctcgat
     6241 ggggtcgaag aagtgccgcg tgtaggccat gaaggcgacc agggtgccga tggcgatggc
     6301 gccgccgagc agatccagcc cgccgatggc gagcgccaaa cccgtggcga ggctcgccag
     6361 ggtgatgacg atgg
//
LOCUS       DBNHOX010000504         2480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1647012, whole genome shotgun sequence.
ACCESSION   DBNHOX010000504 DBNHOX010000000
VERSION     DBNHOX010000504.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1647012"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..2155
                     /locus_tag="AC16UZ_11070"
     CDS             89..2155
                     /locus_tag="AC16UZ_11070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR6247268.1"
                     /translation="MNDHRHHHHGHEAHGDHQSHQGAVDHRAHSPHDAHAGHSVAMFR
                     SKFWLSLALTVPVVIWAEHVQALLGYGAPAFPGSTWIGPALSTVIFFYGGWVFVRGAA
                     GEVRAKLPGMMTLISLAIVVAFGFSWVVELGLLDAEPLWWELATLVTIMLLGHWIEMR
                     SISQAQGALQELAKLLPDTARRIREDGTEEEVAVSEIRRGDLVLVRPGESVPVDGVVR
                     KGESALNEAMITGESRPVKKGEGDEVIAGTINGQGSLRVETTGTGEETTLSGIMRLVA
                     DAQQSKSRAQHLADRAAQMLTGVAVAAGIVTLVTWQFIGAPLDFAIVRTVTVLVIACP
                     HALGLAVPLVVAISTTLGAQNGLLVRDRRGLEEARNLDVVVFDKTGTLTLGEFRVVDL
                     AVAEGLSEEEALRIAAGVEVESEHPIARGIVSTARERGVAKSSVDGFRAITGKGVAAT
                     VDGQEYFVGGPALLEAEAASVPTALREAADRAATRGQAAIYLLRGGDALAVFVVADAI
                     REESREAVNALHERGVQVAMLTGDAQAVADAVAEELGIDTVFAEVLPEDKASRIRELQ
                     DRGQQVAMVGDGVNDAPALATADIGIAIGAGTDVAVEAGHIVLVRSDPRDIPRIISLS
                     RATYRKMVQNLWWAAGYNILAIPLAAGALAAWGILLSPAVGAVLMSASTVIVAINAQL
                     LKRARL"
BASE COUNT          374 a          819 c          873 g          414 t
ORIGIN      
        1 agcgacttgc caggaagcct gtccgttccc tccgtgggcc tggctagact tgtagctgcg
       61 ggcacggctt ctcgaccagg agcagaccat gaatgaccat cgccaccatc accacggcca
      121 cgaagcccac ggtgatcacc aaagccacca aggcgctgtc gaccaccgag ctcacagccc
      181 gcacgatgcc catgccggcc acagcgtcgc catgttccgg agcaagttct ggctttccct
      241 cgcgctgacg gtgccggtgg tgatctgggc cgagcacgtc caagcgctgc tcggctacgg
      301 ggcgcccgcc ttcccgggct cgacgtggat cgggcccgcc ctgtccaccg tgatcttctt
      361 ctacggtggc tgggtgttcg ttcggggcgc ggcaggagag gtccgggcga agctgcccgg
      421 catgatgacg ctcatctcgc tggccatcgt cgtggccttc ggcttctcat gggtggtcga
      481 gctgggcctg ctggacgccg agccgctgtg gtgggagttg gcgacgctgg tgacgatcat
      541 gctgctgggc cactggatcg agatgcgctc gatcagccag gcccaggggg cgctgcagga
      601 gctggcgaag cttcttcccg acacagcgcg tcgcatccgg gaggacggta ccgaggaaga
      661 ggtcgcggtc tcggagattc gccgaggcga cctggtgctc gtgcggcctg gggagagcgt
      721 ccccgtcgac ggcgtcgtcc gcaagggtga gagcgcgctc aacgaggcga tgatcacggg
      781 cgagtcccgg cccgtgaaga agggcgaggg cgacgaggtc atcgccggaa cgatcaacgg
      841 tcagggctcc cttcgcgtcg agacgacggg gacgggggag gagaccacgc tatcgggaat
      901 catgcgcctg gtcgccgacg cgcagcagtc gaagtctcgg gcgcaacacc ttgccgatcg
      961 ggcggctcag atgctgaccg gcgtggccgt cgccgcgggc atcgtcaccc tggtcacgtg
     1021 gcagttcatc ggtgcgccgc tggacttcgc catcgtacgg acggtgacgg tgctggtcat
     1081 cgcgtgtcct cacgcgctcg gcttggccgt cccactggtg gtggccatct ccaccacgct
     1141 cggggcgcag aacgggctcc tcgtgcggga ccggcgcggg ctcgaggagg cgcgcaactt
     1201 ggacgtcgtc gtcttcgaca agacgggcac cctcaccctc ggcgagttcc gtgtggtgga
     1261 cctggccgtc gcggaaggtc tgtccgaaga ggaggcgctg cgcatcgccg ccggtgtgga
     1321 ggtcgaatcc gagcacccca tcgctcgtgg gatcgtgagc acggcccgtg agcgaggagt
     1381 ggcaaagagc tccgtggacg ggtttcgcgc catcaccgga aagggtgtag ccgccaccgt
     1441 tgacgggcag gagtacttcg tgggcgggcc ggcgctcctc gaagccgaag cggcgtccgt
     1501 gcccaccgcc ctccgggagg ccgccgatcg tgcggcgact cgtggtcagg cagccatcta
     1561 cctgctgcgg ggcggtgacg ctttggccgt cttcgtggta gcggacgcca tccgcgagga
     1621 aagtcgcgag gccgtgaacg cgctgcacga gcgtggcgtg caagtcgcca tgctcacggg
     1681 tgacgcccag gcggtggccg acgcggtggc cgaagagctg ggcatcgaca ccgtcttcgc
     1741 cgaggtgctg cccgaggaca aggcctcacg catccgagag cttcaggacc ggggccagca
     1801 agtggcgatg gtcggcgacg gcgtcaacga cgctcccgcg ctggcgaccg ccgacatcgg
     1861 gatcgccatc ggggccggta ccgacgtcgc cgtcgaggcg ggccacatcg tcctggttcg
     1921 atcggacccc cgggacatcc ccaggatcat ctccctgtcc cgggccacgt accgcaagat
     1981 ggtccagaac ctatggtggg cggccggcta caacatcctg gccattccac tggccgccgg
     2041 ggcgctggcc gcatggggca tcctgctgtc accggccgtg ggcgcggtgc tcatgtcggc
     2101 cagcaccgtg atcgtggcca tcaacgctca gctgttgaag agggcgaggc tttgagggtc
     2161 gctcggcagc ccaggcgttc gtgccgaccc ggccacaagc gcgaatgact cctgcattga
     2221 cgcgctcggg tggcacggcc catttggggt cttggccatg accttacccg gggtcgggcc
     2281 ttcgccacac ctgccgcggt aactcatgtc ctcgtcctcc aagtcctctg gcagcctccg
     2341 agcctcgcgt gtccaccgtc ggctcgggat gatcgcgacc gtccccatcg tcgggtggat
     2401 cgcgtcttcc ttcgtgcttc atggtgtagg cctcacgctc ccgaacgggc tccaaggcgt
     2461 gtacgagctc gagtcgacgc
//
LOCUS       DBNHOX010000505         1775 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1711582, whole genome shotgun sequence.
ACCESSION   DBNHOX010000505 DBNHOX010000000
VERSION     DBNHOX010000505.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1711582"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1003)
                     /locus_tag="AC16UZ_11075"
     CDS             complement(77..1003)
                     /locus_tag="AC16UZ_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247269.1"
                     /translation="MLVVTELISPAPARAYETDQFSRRLEPLADSTVVLDQRVNESIA
                     AAARTHRGSRNDSRVVDAIYHDIGGHHWVDKIERWAMKSDEVDRNTPDRYDSIYQGHP
                     LWATRVAALFGVGPTIKLNGTRIGSDKLGHFLSQGRKFWRRYQRSLDEGEAAEHSAYT
                     ERALFGQMTTGVYSNADLVANYEGYRFYRSLFEDDVVPGKPAILAWDGDHWQVQRPFT
                     WADHVNAYWDEALNVNHFDALLYPHMKQRLLGYCDDYFEAPERYDVNDEEVLQARYSE
                     LQLRETSELRLSNLCPAEGPAPAVTATGQGSR"
     gene            complement(1046..>1775)
                     /locus_tag="AC16UZ_11080"
     CDS             complement(1046..>1775)
                     /locus_tag="AC16UZ_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247270.1"
                     /translation="SAGPLFMKDLLQGREFFAPWGVGADYFTMKQDYKIQSLQFALPG
                     VSIDDPSLIGVTNELDHYDLKLDVWVTPFLNVFGLIGQVDAKTYVDLSGVPVAGLPVS
                     LGTLTVAYDGEVYGAGFNLFYGTDRWFAALSNTWTDASLSGDFDSSVKTFSSQPRLGL
                     IFNQSSLWLGAMYLDTEESHAGTIGLPIPGIPPVPFSVELASMKAWNYAIGIGHVFSP
                     KAHFSLEVGFGDRNHTLANFTYRF"
BASE COUNT          310 a          528 c          604 g          333 t
ORIGIN      
        1 gaggaagtca aggcttacat ggcggagtcc tatccgcggg tgatcgtgtt cgagcggccg
       61 ctggaccgga tccgcttcag cgcgaacctt gacccgtcgc cgtgaccgct ggtgcgggcc
      121 cttctgccgg acacaggttg ctgagcctca gctccgaggt ttcgcgcagc tgcagctccg
      181 agtagcgcgc ctgcagcacc tcttcgtcgt ttacgtcata gcgttcgggg gcctcgaaat
      241 aatcgtcaca gtagcccagc aggcgctgct tcatgtgcgg gtacagcagc gcgtcgaagt
      301 gattgacgtt gagcgcctcg tcccagtagg cgttgacgtg gtccgcccag gtgaacgggc
      361 gctggacttg ccagtggtcc ccgtcccagg cgaggatcgc cggcttgccg ggtacgacgt
      421 cgtcctcgaa caggctgcgg taaaagcggt aaccctcgta gttggcgacc aggtcggcat
      481 tcgagtacac gccggtggtc atctggccga acagagcgcg ttcggtgtag gcggagtgct
      541 cggccgcctc gccctcgtcg agggagcgct ggtagcggcg ccagaatttg cgcccttgcg
      601 agaggaagtg gcccagtttg tccgaaccga tgcgggtgcc gttgagcttg atggtgggcc
      661 ccacgccgaa cagggccgcg acgcgggtgg cccagagggg atggccctgg tagatggagt
      721 cgtagcggtc cggcgtgttg cggtcgactt catcggattt catggcccag cgctcgatct
      781 tgtcgaccca gtggtggccg ccgatgtcgt ggtagatggc gtcgaccacc cgcgaatcgt
      841 tgcgtgaacc gcggtgtgtg cgcgcggccg cggcgatgga ttcgttgacg cgctggtcca
      901 gcaccaccgt ggagtccgcc aggggctcga ggcgcctcga aaactggtcc gtttcgtagg
      961 cgcgggccgg cgcgggcgag atcagctccg tgacgacgag catgcctagg agagttgcct
     1021 gcgccagcac gcggatcatc ggtgcctaga agcggtaggt gaagttggcc agcgtgtggt
     1081 tgcggtcgcc gaagccgact tccaggctga aatgggcttt ggggctgaat acgtggccta
     1141 tgccgatggc atagttccac gccttcatgc ttgccagttc cacgctgaag ggcaccggcg
     1201 gaatcccggg gatcggcaaa ccgatggtgc cggcatggga ttcttccgtg tcgaggtaca
     1261 tcgcgccgag ccacaggctc gactggttga agatcaaccc gaggcgaggc tgtgacgaga
     1321 aggtcttcac ggaggaatcg aaatctccgc tgaggctggc gtcggtccag gtattgctga
     1381 gtgcggcgaa ccagcggtcc gtgccgtaga acaaattgaa tcccgcacca tagacctctc
     1441 cgtcgtaagc gaccgtcagg gtccccaggg agaccggcaa gccagccacc ggcaccccgc
     1501 tgagatcgac ataggtcttg gcgtccacct ggccgatcag gccgaacacg ttgaggaagg
     1561 gtgtcaccca gacgtccagt ttgagatcgt agtgatccag ctcgttggtc accccgatca
     1621 gcgaggggtc atcgatgctc acgcccggca gagcgaattg aagcgactgg atcttgtagt
     1681 cctgcttcat cgtgaagtag tcggccccga ccccccacgg tgcgaagaat tcccgcccct
     1741 gcagcaggtc cttcatgaac agcggccctg cggac
//
LOCUS       DBNHOX010000506         2543 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_104857, whole genome shotgun sequence.
ACCESSION   DBNHOX010000506 DBNHOX010000000
VERSION     DBNHOX010000506.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_104857"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="AC16UZ_11085"
     CDS             <1..579
                     /locus_tag="AC16UZ_11085"
                     /inference="COORDINATES: protein motif:HMM:NF040142.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase CrtO family protein"
                     /protein_id="HIR6247271.1"
                     /translation="LRSAVALVATAIGFGVSFFMLQSAIGTASPWLGLLLMFYFMGLA
                     KVAEPLFMLRIPKALKAVRAWETSGPAYGRLAVHRFGKVLRNTPLRHLNSSVYLANRR
                     SDLRTLYRYAESAEAIHFWAMVLFTPYIAFVWLQRQVVLTSVFVLIQLLCNIYPILHL
                     RTLRGRLDELFVRQHAKRYTKPVAGDAAHRLR"
     gene            complement(943..>2543)
                     /locus_tag="AC16UZ_11090"
     CDS             complement(943..>2543)
                     /locus_tag="AC16UZ_11090"
                     /inference="COORDINATES: protein motif:HMM:NF015585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR6247272.1"
                     /translation="GVQVGEVWLCSGQSNMGFPLKNANGGPEAGADAFNHNIRLFRMT
                     AGNGPATTSWRIAEAASAGDFSAVCYWMGLELSQFLDEVPIGLVQATHDGTSIDHWQH
                     SSNGIGDDYDAMVRPIQPYAVKGVLWYQGESNGGDAGYADKLRNMIAEWRGDWGQAAL
                     PFGIVQLAYRSGWNVARNAQLEVADSTEDCFLVVIRDLPGGSLHPPEKKPVGIRSAIG
                     ARGLLYGDNITWSGPTRDVEKSYVENGAVLLHWKHVGNGLFTDDGLAPGTFLVAGATG
                     RFVPAEAEIDGDTVRVWSSSVDDPVRVQYSYKSVGNLHNRISVPTEGGGAYVDRLKAS
                     EFEITLAGSTGNQPPVARFTYQVSGLTVDFDALTSADADGTIAGYVWNFGDGATGIGA
                     TPSHDYAASGTYPVVLTVTDNAGASGQTQKDINVSSGGGGGSTVHVASIITYTQNVDR
                     GVKRGVAEVVIHDDLGSPVAGALVTVEFSGTFTETASGTTDGSGTARASTDAMAKGSV
                     SVSACVTNVTYAPLVYDPTVACSP"
BASE COUNT          432 a          833 c          769 g          509 t
ORIGIN      
        1 ctccgcagcg ccgtcgcatt ggtcgcaacc gccatcggat ttggcgtcag cttcttcatg
       61 cttcaaagtg ccattggcac cgcgtctcct tggcttggtt tgctgttgat gttctatttc
      121 atgggtctgg ccaaagtagc tgagccgctt ttcatgcttc ggattcccaa ggcgctcaaa
      181 gcggtccgtg cctgggagac gtctggccca gcctacggcc ggctggctgt ccatcgcttt
      241 ggcaaggttc tgcggaacac tccactccgc catctcaact cttccgtcta cctcgcaaac
      301 aggcgaagtg acctgcggac cctctatcga tacgccgagt cggctgaggc catccacttc
      361 tgggcaatgg tcctgttcac gccatacatc gcatttgtct ggctccaaag acaggtagtg
      421 ttaacgtcgg tcttcgttct gattcaacta ctgtgcaata tctatcccat cttgcacttg
      481 cgcaccctac gcgggcgtct tgatgagcta tttgtgaggc agcatgcgaa acgctacacc
      541 aagcctgtag ccggcgacgc cgctcatcgc ctgcgttaga gagtttctgc ttctggccga
      601 caacggacat ccctcagagt tcggttgcga gacgctagcc aatttgtcgc tgggtgagac
      661 agaccccggc cttgtccctg ggcttcctca ggttgggcac ttcggcacgc cccgcagata
      721 aggggggcag cgcccaggcc atcgcagctc aacatcaaca aattcaggac tgttcgaaat
      781 tcaactcacg cccccgctac caacctttca aggaaccccg gccccgcgcc ggggtttctt
      841 tattccgcca atgcctgttc ctggccagaa gcaggcatcg gctcaagaga gaaatccctg
      901 ctgcgaccgc gcaatgccgt gtgggctgcc ccacgtgagc cgctacggtg agcaggctac
      961 cgtcgggtca tagaccagcg gggcgtacgt gacgttggta acacacgcgc tgacgctgac
     1021 cgagcccttg gccatggcgt cggtcgatgc gcgtgccgtg ccgctgccat ctgtcgtgcc
     1081 gctcgccgtc tcggtgaagg tgccactgaa ctctactgtg accagagcac ctgctacggg
     1141 gctgcccagg tcgtcgtgaa tcaccacctc ggccacgcca cgcttgacgc ctcgatcgac
     1201 gttctgggtg taggtgatga tgctggcgac gtgcacggtg ctgccaccac cgccaccgct
     1261 cgagacgttg atgtctttct gtgtctgacc gctggcgccg gcattgtcgg tgacggtcag
     1321 caccacgggg tacgtgcccg aagccgcata gtcgtggctc ggcgtcgcgc caattccggt
     1381 cgcgccgtca ccgaaattcc acacatagcc cgcaatcgtg ccgtcggcgt cggcgctggt
     1441 taatgcatcg aagtcgacgg tgagcccgga gacctggtag gtgaaccggg cgacgggcgg
     1501 ctggttgccc gtgcttccgg ccagcgtaat ctcgaactcg gacgccttca ggcgatccac
     1561 gtaggcgccc ccgccctcgg tcgggacgga gatccgattg tgcaggttgc ccacactctt
     1621 gtaggaatac tggacgcgca ccgggtcgtc gacggacgaa ctccagacgc gcacggtgtc
     1681 gccgtcgatc tctgcctcgg cgggcacgaa gcggccggtc gcgcctgcga ccaggaaggt
     1741 gccgggcgcg agcccgtcgt ccgtgaacag cccgttgccg acgtgtttcc aatgcaaaag
     1801 gacggcgcca ttctcgacat aggatttctc gacatcccga gtgggcccgg accacgtgat
     1861 gttgtccccg tacaggagcc cccgagcccc gatggcagaa cgaatgccga ccggcttctt
     1921 ctcaggcggg tgcagggagc cgcctggcag gtcacgaatg acgacgagga agcagtcttc
     1981 cgtggagtcg gcgacctcga gctgcgcgtt gcgcgcgaca ttccagcctg aacggtaggc
     2041 cagctgcacg atgccgaatg gcagcgcggc ctgtccccag tcgccgcgcc actcggcgat
     2101 catgttcctt aacttgtcgg cgtagccggc atcgccgccg ttgctctcgc cctggtacca
     2161 cagcacgccc ttgaccgcgt agggttggat cgggcggacc atggcgtcgt agtcgtcgcc
     2221 gatgccgttg ctgctgtgct gccagtggtc gatcgaggtg ccgtcgtggg tggcctggac
     2281 caggccgatc ggcacctcgt caagaaactg cgacagctcc agtcccatcc agtagcagac
     2341 cgccgaaaaa tcgcctgcgg aggcggcctc ggcgatccgc cagctcgtcg tcgccggccc
     2401 gtttcccgcg gtcatgcgga agaggcgaat gttgtgattg aaggcatccg cccccgcctc
     2461 ggggccgccg ttggcattct tcagcgggaa gcccatgttg gactggccgc tgcacagcca
     2521 tacctcgcca acctggacgc ctg
//
LOCUS       DBNHOX010000507         2222 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1004959, whole genome shotgun sequence.
ACCESSION   DBNHOX010000507 DBNHOX010000000
VERSION     DBNHOX010000507.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1004959"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1872
                     /locus_tag="AC16UZ_11095"
     CDS             <1..1872
                     /locus_tag="AC16UZ_11095"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6247273.1"
                     /translation="DWDFNDTMTFTSLTSFREVDSELNWVIATEDVARLRLSTGTVPL
                     AVYGSNDYVDDSETFTQEFRLTSSGSGPFQWVGGLYYLNEETYRDETVPAGLLVPDGD
                     GGALVLVPAADAGDKQDNTTDSYAIFGQVTWDITDKLAITAGGRQDWEEKKISRLRTP
                     NGLAPARIFDFSTSEDWSAFTAKGGIEFQATDDLFFYATVSEGFKSGGYQGSAATELA
                     AITPFEPEGSPFGTPGQWVIGMGYDDSLIEEQRHPERSDLDAISSEHPIALIHVSGHL
                     TTVNSMALDVSGITAETPDPPGGAIRRQPGSNEPNGVMEETATAPLRHIYSGGYGGGG
                     GAPGADLRKALAMYAGNGVTTVQDGGASWEIHEMVREASIAGDVSLDVVLYPVGMDPT
                     FSVPEGVTLGEYQDRVKVGGIKLLLDGSPQGKTAYLSKPYEVAPHGQPDDYRGYPMIP
                     QAMADERVAHFIGRGIPMLVHCNGDAAAEMLLDALDKANETMDLGDHRTVMIHAQTVR
                     EDQLDRMAALGVVPPDMMRLLWATVNRITRSGQTLGEAQRISALEAIRAVTVHAAYQN
                     FEEYLKGSLTPGKQADLVILSANPVTVDPQQILDIEVVETISRGVTVYQEPSLSASL"
     gene            1921..>2222
                     /locus_tag="AC16UZ_11100"
     CDS             1921..>2222
                     /locus_tag="AC16UZ_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HIR6247274.1"
                     /translation="MSRYRPPRPRSSPYITPAGQERLAAELKELWKVKRPEVTQKVAE
                     AAALGDRSENAEYIYGKRQLREIDARIRFLAKRLDELTVVDRPPRDQGKVYFGAWV"
BASE COUNT          418 a          738 c          720 g          346 t
ORIGIN      
        1 gactgggatt tcaacgacac catgacgttc acctcgctga cctccttccg cgaggtggat
       61 tcggagctga attgggtcat cgccaccgag gacgtcgccc ggctgcgact ctccaccggg
      121 acggtgccgc ttgccgtcta cggcagcaac gactacgttg acgactcgga aaccttcacg
      181 caggagttca ggctcacctc gtccggcagc gggcctttcc aatgggtggg cgggctctac
      241 tacctgaacg aagaaaccta ccgcgacgag accgtacccg ccggcctgct ggtgcccgac
      301 ggcgatgggg gcgccctcgt tctcgtgccg gccgcggatg cgggcgacaa gcaggacaac
      361 accaccgaca gctacgccat attcggtcaa gtcacctggg acatcacgga caagctggcg
      421 atcaccgccg gtggccgtca agactgggaa gaaaagaaga tcagccggtt gcgcacgccg
      481 aacggcctgg cgccggcgcg gatcttcgat ttcagcacca gcgaggactg gagcgccttc
      541 acggctaagg gcggcatcga gtttcaggcg accgacgacc tgttcttcta cgcaaccgtc
      601 tccgaagggt tcaagtcagg cggctaccag ggtagcgcgg ccacggagct cgccgccatt
      661 acgcccttcg agcccgaggg ctcgcctttc ggcacgccgg gccagtgggt gatcggcatg
      721 ggctacgacg attcgctcat cgaggagcag cgccatccgg agcgttccga cctggacgcg
      781 atctcaagcg agcatcccat cgcgctcatc cacgtttccg gtcatctgac caccgtgaac
      841 tccatggcgc tggacgtaag cggcatcacg gccgagacgc ccgatccccc gggcggcgcg
      901 atccgccgcc agccgggcag caacgagccc aacggcgtta tggaggagac ggctaccgcg
      961 ccgttgcgcc acatctattc tggcggctat ggcggcggcg ggggggcgcc cggtgcggac
     1021 ctgcgcaagg cccttgcgat gtacgccggg aacggggtca ccacggtcca ggacgggggc
     1081 gcttcctggg agatccacga gatggtccgg gaagcatcca tcgccgggga cgtcagtctc
     1141 gacgtggtgc tgtaccccgt gggtatggac ccgacgtttt ccgttccgga aggcgtcacg
     1201 ctgggcgagt accaggaccg ggtgaaggtc ggtggcatca agctgcttct ggatggttcg
     1261 ccccagggca agacggccta cctgagcaaa ccctacgagg tggcgcccca cggccagccg
     1321 gacgattacc gcggctatcc catgattccg caggccatgg cagacgagcg ggtggcccac
     1381 ttcatcggcc gcggcatccc catgctggtg cactgcaacg gcgacgctgc cgccgagatg
     1441 ctgctcgacg ccctggacaa ggcgaatgag accatggatc tgggcgatca ccgaacggtg
     1501 atgatccacg cccagacggt tcgggaagat cagctcgacc gcatggcggc actcggcgtg
     1561 gtgccgcccg acatgatgcg tctgctgtgg gccacggtga atcgcatcac ccgcagcggc
     1621 cagaccctgg gcgaggctca gcgcatcagc gccctggagg cgatccgcgc ggtgaccgtc
     1681 cacgccgcct accagaattt cgaggaatac ctcaaagggt ccctgacacc gggcaagcag
     1741 gcggatctgg tgatcctctc ggccaacccg gtcaccgtgg acccccagca gattctggac
     1801 atcgaggtgg tggaaacgat ctcccggggt gttaccgtct accaggaacc ctcgctctcc
     1861 gcgagccttt aggccggacc caatgtctgc tggcaaaaaa accttaacgc acgggcgccg
     1921 atgagccgct accgcccgcc ccggcccaga tcctccccct acatcacgcc ggccggccaa
     1981 gagcgtctgg ctgccgaact caaggagctg tggaaggtca aacgccccga ggtgacccaa
     2041 aaggtcgctg aagcagccgc gctgggcgac cgttccgaga atgccgagta catctacggc
     2101 aagcggcagc tcagagagat cgacgcccgc atccggtttc tcgccaagcg gctggacgag
     2161 ctgacggtgg tggaccgccc cccgagggac cagggcaagg tctatttcgg cgcgtgggtg
     2221 ag
//
LOCUS       DBNHOX010000508         4403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_362388, whole genome shotgun sequence.
ACCESSION   DBNHOX010000508 DBNHOX010000000
VERSION     DBNHOX010000508.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_362388"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="AC16UZ_11105"
     CDS             complement(<1..556)
                     /locus_tag="AC16UZ_11105"
                     /inference="COORDINATES: protein motif:HMM:NF042569.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase kinase/phosphatase AceK
                     regulatory subunit"
                     /protein_id="HIR6247275.1"
                     /translation="MTRGKSAMASAADGIARAIAMGYESYQAEFQAITAMAQSRFEGQ
                     DWAGVQDAGARRLAIYGEHVRDVVAEVHTLTGGSEIDASLWRKSKQAFTSIISEHYNA
                     ELYETFYNSIHREMTDDSVVDDTEMFVRTLFPIPPVQPAEPVFERHAIAGEPEEFVRT
                     LLDGLAINRPWVDRDRDVRNILRSL"
     gene            complement(558..1409)
                     /locus_tag="AC16UZ_11110"
     CDS             complement(558..1409)
                     /locus_tag="AC16UZ_11110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6247276.1"
                     /translation="MGTRCFLVSGTTSGIGLATARALAATGGTVVTANRDPDRGESTA
                     ALLRDATSNPNVINTVCNLSSLASVRSCAARIREQFPRIDVLINNAGVWTAKPQLSED
                     GFELSFATNHLGPFLLTHMLLEPLRAAGGARIVNVASVQHRAADLDLDHLTPKTPYRG
                     LRAYSRSKLANVMTTLAFARRFDAAEISANCLHPGVVASNIVPENSPLLRRAARVVSG
                     LMRSPARGAATSIYLATSPEVTGVTGKYFDPDQRIIQPSAEARDEAAQDLLWERSLEL
                     TGLAEAG"
     gene            complement(1411..1797)
                     /locus_tag="AC16UZ_11115"
     CDS             complement(1411..1797)
                     /locus_tag="AC16UZ_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247277.1"
                     /translation="MTGPGLTRPGIRLLGLAALGLCVLAYDAGSYTLLHRLVLPIAMG
                     VAAGLMVQSAAAVLLAGTLLAGVHARPADPDWVIGIAYPALAALCGVGLTWIVVLRFR
                     RRIKATREARWRGRRERSAAPEGSES"
     gene            complement(1794..2570)
                     /locus_tag="AC16UZ_11120"
     CDS             complement(1794..2570)
                     /locus_tag="AC16UZ_11120"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6247278.1"
                     /translation="MDLGLTGKTALVSGSYRGTGLVIARHLAAEGVRVFVHGLEPGQA
                     ERAVEEIGSGDPVTGDITCDAGAREVAEALGDSRVDILVNNYGTADRGTWQDSDTDDW
                     LDAYQKNVLSAQRLIRAYFAPMRERGWGRIINLGTVGSTRPNSRMPHYYASKGALATM
                     TISLAKEASGTGVRVNLVSPGLILTPEVEAAYLERGRAKGWGETWEEVEPSVAADIPI
                     GRIVRREEVADLVTFLASPLADAIHGQNIRVDGGALDVLS"
     gene            2652..3404
                     /locus_tag="AC16UZ_11125"
     CDS             2652..3404
                     /locus_tag="AC16UZ_11125"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR6247279.1"
                     /translation="MDPWTLGIFLGGALFGSYVQSVTGFAMAMILVAVASAAGNSQVS
                     VVAAVVSLLALVNVALALRGHLERVEARLFVWLAVGQLPAIWAGIYLLEVLEGQAVRA
                     LEVVLGVFIVLGSLSMMLRPQPRPSTSPPWACVAAGVTGGLAGGLFAASGPIMGWFNY
                     RQPLPVAAIRATLLCGFAMTTSVRTVLVGVNGGLTGEVWLLTGLALPVVVLGTWAGRT
                     FEPPVSELAMKRLAFAVLLVMGIWILLRAALG"
     gene            complement(3401..3892)
                     /locus_tag="AC16UZ_11130"
     CDS             complement(3401..3892)
                     /locus_tag="AC16UZ_11130"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6247280.1"
                     /translation="MTDLRRAGEDDLPALVRIYNHYVAHSHCTFDIETYSVDERRGWW
                     RRFDAARYQCWIAEHAGRVIGYACSQPLKAKGGYETSVEVSVYVDAAHTGSGTGRSLY
                     QQLLAALAPEDVHRAYALVALPNDPSIALHQTMGFREVAHLGEVGRKFGRYWDVVWLE
                     RAL"
     gene            complement(3889..>4403)
                     /locus_tag="AC16UZ_11135"
     CDS             complement(3889..>4403)
                     /locus_tag="AC16UZ_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6247281.1"
                     /translation="TFEGELGLTVGDKAVLLKQVGPAHTRGDVLAYVPDEKLIYTGDI
                     LFIEGHPILWVGPVGNWIEACDYMLGLDLENVVPGHGPVTDKRGVMAVRDYLIYIRDE
                     ARKRFDAGMPPDEAAVDISLTDYDSWGDAERIVVNVAVLYKEFAADPEPGDIAELFAL
                     MAKVHKARRR"
BASE COUNT          653 a         1438 c         1508 g          804 t
ORIGIN      
        1 aaagagagcg cagtatgttc cgcacgtcgc ggtcccggtc gacccatggc cggttgatgg
       61 ccagaccgtc gagcagcgtg cgcacgaact cttcgggttc gccggcgatg gcgtgtcttt
      121 cgaagaccgg ctcggctggc tgcaccggcg ggatggggaa cagggtacga acgaacatct
      181 ccgtgtcgtc gactacgctg tcgtcggtca tctcgcgatg aatggagttg tagaacgtct
      241 cgtagagctc ggcgttgtag tgctcgctga tgatcgatgt gaacgcctgc ttggatttgc
      301 gccacagcga agcgtcgatc tcggaccctc ccgtgagcgt atggacctca gcgaccacgt
      361 cgcgcacgtg ctccccgtag atggccaggc gacgcgcccc cgcgtcctga acgccagccc
      421 agtcctggcc ttcgaaacgg ctttgggcca tggcggtgat ggcctgaaac tccgcctgat
      481 aggattcgta ccccatggca atcgctcggg cgatgccgtc ggccgcggac gccatggcgg
      541 atttgcctcg tgtcatttca gccggcctcc gccagcccgg tcagctcgag gctgcgttcc
      601 cagagcaggt cctgtgccgc ctcgtcccgg gcttccgcgg agggttggat gatgcgttgg
      661 tcggggtcga agtatttgcc cgtcacccct gtcacctctg gcgacgttgc caggtagatg
      721 gaagttgcgg ccccgcgggc ggggctgcgc atcaggccgc tcacgacacg cgcagctctg
      781 cgcagcaggg ggctgttttc gggaacgatg ttggaggcaa ccacgccagg atggaggcag
      841 tttgcggaga tctccgcggc gtcgaagcgc ctggcaaagg cgagcgtcgt catcacgttg
      901 gcgagctttg accggctgta ggccctcaga ccccgatagg gcgtctttgg ggtcagatga
      961 tccaggtcca ggtccgccgc gcggtgctgc accgaggcga cgttcacgat ccttgcgccg
     1021 ccggctgctc tcagcggctc gaggagcatg tgggtgagca gaaacggccc gaggtgattc
     1081 gtggcgaaag aaagctcgaa gccgtcctcg gaaagctgag gcttggcggt ccacacgcca
     1141 gcgttgttga tcagcacgtc gatgcgcggg aactgctccc ggatgcgggc tgcgcaggag
     1201 cggacagatg ccaacgaaga caggttgcac acggtgttga tgacgttggg gttgctggtg
     1261 gcatcccgca gcagggcggc cgtgctctcg cccctgtcag ggtcgcggtt tgccgtgacg
     1321 acggtgcctc cggtggccgc gagggcgcgt gcggtggcca gaccgatgcc gctggtggtg
     1381 ccgctgacca ggaagcagcg tgttcccact tcagctctcc gatccttccg gcgctgccga
     1441 gcgctcgcga cggccgcgcc agcgtgcctc gcgcgtggct ttgatccgcc ggcggaaacg
     1501 cagcacgaca atccaggtca gccccacgcc acacagagcg gccagcgccg gataggcgat
     1561 ccctatgacc cagtccgggt ccgcaggacg ggcgtgaacg ccggcgagca gggtgccggc
     1621 gagcagaacg gcggccgcac tctgcaccat caggcctgca gccacgccca tggcgatggg
     1681 caggacgaga cggtgaagga gggtgtagct gcccgcgtcg taggcgagca cacacaggcc
     1741 cagcgcggcc agcccgagca gccggatacc cggtctggtc aggcctggtc cggtcaagac
     1801 agcacgtcca acgccccgcc gtccacccgg atgttctggc cgtgaatggc gtcggccagt
     1861 gggcttgcca ggaacgtgac gaggtcggcc acctcctcac gtcggacgat gcgcccgatg
     1921 ggaatgtcgg ccgccacgct tggctccacc tcttcccagg tttctcccca acccttggct
     1981 cggccgcgct cgaggtaggc ggcctccacc tcgggggtga ggatcaggcc gggagaaacc
     2041 agattcaccc gcactcccgt gcccgatgcc tccttggcaa ggctgatggt catggtggcc
     2101 aacgcgccct ttgacgcgta gtaatggggc atccggctgt tgggtcgggt ggatcccacg
     2161 gtcccgaggt tgatgatcct tccccagccc cgctcccgca ttggagcgaa gtacgcccgg
     2221 atcagacgct gagcggagag cacgtttttc tggtaggcgt ccagccagtc gtcggtgtcg
     2281 ctatcctgcc aggtaccccg gtccgcggtt ccgtagttgt tgacaagtat gtcgacgcgg
     2341 ctgtcgccca gggcctcggc cacctctcgg gcgccagcat cgcaggtgat gtcgccggtc
     2401 accgggtcgc cgctgccgat ttcctccacg gcgcgctccg cctgaccggg ctccagcccg
     2461 tggacgaata cccgcacgcc ctcggctgcc agatggcggg cgatgacgag cccggtcccc
     2521 cgatagctgc cgctgacgag cgccgtcttg cctgtgaggc cgagatccat tcctgctttt
     2581 ccgtcgtgcc cccgatggag gccgggacta taccacccga tctgccgcga tcgcctatac
     2641 tgccgtccgc gatggacccc tggactctcg gcatcttcct tggcggcgcg ttgttcggca
     2701 gctacgtgca gtccgtgacc ggcttcgcca tggccatgat cctggtggcc gtggccagcg
     2761 ccgccggcaa ttctcaggtc tcggtggtgg cggcggtggt cagcctgctc gctctcgtga
     2821 acgtggcgct cgcgctgcgc ggtcatctcg agcgggtgga ggcgcgtctt ttcgtctggc
     2881 tcgcggtcgg tcagctgccg gccatatggg ctggcatcta tcttctggaa gtgctggagg
     2941 gccaggccgt gcgggcgctg gaggtcgtgc tcggcgtctt catcgtgctc ggcagccttt
     3001 ccatgatgct gcgcccgcag ccgaggccct ccacgtcacc cccgtgggcc tgcgtggccg
     3061 caggcgtgac cggcggcctg gccggtggcc tgttcgcggc gtcgggtccg atcatgggat
     3121 ggttcaacta ccggcaaccg ctgcccgtgg cggccattcg ggccacgctg ctgtgcggct
     3181 tcgccatgac gacgtcggtc cgcacggtgc tggtgggggt caatggcggg ttgacggggg
     3241 aggtgtggct gctcaccggg ctggctttgc ccgtggtggt gctgggaacc tgggcgggcc
     3301 gcaccttcga gcccccggta tctgagctcg ccatgaagcg gctggcgttc gccgtgctcc
     3361 tcgtcatggg tatctggatc ctgctgcgcg ccgcgctcgg ctaaagggcg cgttccagcc
     3421 agaccacgtc ccagtagcgg ccgaacttcc ggccgacctc gccgaggtgc gccacctccc
     3481 ggaagcccat cgtttgatgc aaagcgatgg agggatcgtt gggcagggcg accagggcgt
     3541 aggccctgtg cacgtcctcg ggcgcgagcg ctgcgagcag ctgctggtag agggagcgtc
     3601 cggttcctga gcccgtatgg gcggcgtcca cgtagacgct cacctcaacc gaggtttcgt
     3661 aaccgccctt ggctttcagc ggctggctgc aggcatagcc gatgaccctg ccggcgtgct
     3721 cggcgatcca gcactggtat cgggcggcgt cgaaccgccg ccaccaccct cgccgctcgt
     3781 cgacgctgta ggtttcgatg tcgaacgtgc agtgggagtg ggccacgtag tggttgtaga
     3841 tccggaccag ggcaggcaga tcgtcctccc cggcgcggcg caggtcggtc aacggcgccg
     3901 ggccttgtgc accttggcca tcagcgcgaa cagctcggcg atgtcgccgg gctcgggatc
     3961 tgcagcgaac tccttgtaga gcacggccac gttgaccacg atccgctcgg cgtcgcccca
     4021 ggagtcgtag tccgtcagcg agatgtccac agccgcctcg tccggcggca tgccggcatc
     4081 gaatcgtttg cgagcctcgt cacgaatgta gatcaggtag tcgcgcacgg ccatcacgcc
     4141 gcgtttgtcc gtgacgggac cgtgtccggg caccacgttc tccaggtcca gaccgagcat
     4201 gtagtcgcag gcttcgatcc agttgcccac ggggcccacc cagagaatcg ggtggccttc
     4261 gatgaagagg atgtcgcctg tgtagatgag cttctcatcg ggaacgtagg ccagaacgtc
     4321 gcctcgggtg tgggcgggcc cgacctgctt gagcagcacc gccttgtcac cgacggtcag
     4381 tccaagctct ccctcgaagg tac
//
LOCUS       DBNHOX010000509         2495 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1454171, whole genome shotgun sequence.
ACCESSION   DBNHOX010000509 DBNHOX010000000
VERSION     DBNHOX010000509.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1454171"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="AC16UZ_11140"
     CDS             <1..688
                     /locus_tag="AC16UZ_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6247282.1"
                     /translation="GQICLAPDYVFVPEERVDEFVESSARSVEKMYPTLLDNPDYTSV
                     INQRHYDRLQGYVEEAREKGAKVVEVNPANEDFRQQPHHKIPPTLIVDPDESLQVMQD
                     EIFGPILPVKGYANVADTIDYINHHPRPLGLYYFGDDREEEGKVLSHTTSGGVTVNDV
                     VMHVAQEDLPFGGVGPSGMGAYHGHDGFKTFSHAKSIFTQSKVNVAELAGLRPPYGEK
                     MLKAIRAQMR"
     gene            696..1469
                     /locus_tag="AC16UZ_11145"
     CDS             696..1469
                     /locus_tag="AC16UZ_11145"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6247283.1"
                     /translation="MESGSSEIDRLEPRAFERDGFVVVRGCCDPGLVRRIRDRASSYL
                     QPLLGPAEFEADVGYPGSPASRASDGGDTCRRLLHAYARDSVFRDWATGSSVGAVLRV
                     LFGGADVMMSQCHHNCVMTKHPGYSSATLWHQDIRYWSFDRPELVSVWLALGEETEEN
                     GALKVIPGSHAMNIDRGRLDRDLFLRPELAENAELVEHAQTVPLEAGDVLFFHCRLFH
                     AAGKNLTDEVKLSVVTTYHTADNRPIPDTRSAQYPGVPL"
     gene            1477..2079
                     /gene="smrA"
                     /locus_tag="AC16UZ_11150"
     CDS             1477..2079
                     /gene="smrA"
                     /locus_tag="AC16UZ_11150"
                     /inference="COORDINATES: protein motif:HMM:NF033154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HIR6247284.1"
                     /translation="MGDEDVFREAMSDVAPLQRKQRAQPERGPVEATSAQLERREFAL
                     GNRDREAADPNYLTLGEVPQRQPREIVEWKKDGVQNAVFARLKAGKYPVEGSVDLHRQ
                     TVREARAAVFRFLNLAMAKGWRMVLIAHGRGEQSETPARIKSYVAAWLAQVPEVIAYH
                     SAQNNQGGTGACYVLLKKSPQARENNREQYGGKGEAEEPG"
     gene            complement(2104..>2495)
                     /locus_tag="AC16UZ_11155"
     CDS             complement(2104..>2495)
                     /locus_tag="AC16UZ_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6247285.1"
                     /translation="VLELLTTHLKAPDRSGRARGSKEGVRLRYADLRIKAYAARSMVY
                     RTARLADSGSNVINEGIACKVFATETAGEIVDTGIQLVGGSALVESHPLAELYRRLRA
                     LRLAEGGSDVLRLNLARGRLDLDKGRI"
BASE COUNT          445 a          804 c          826 g          420 t
ORIGIN      
        1 gggacagatc tgcctcgctc cggattacgt cttcgtgccc gaagagcggg tcgatgagtt
       61 cgtcgagtcc tcggcgcgtt ccgtcgagaa gatgtacccg acgctgctgg acaatccgga
      121 ctacacgtcg gtgatcaatc agcgtcacta cgaccgcctc cagggctacg tggaggaggc
      181 tcgggagaaa ggcgccaagg tagtagaggt caacccggcc aacgaggact tccgtcagca
      241 accgcatcac aagatcccgc cgacgctgat cgtcgacccg gacgagtcgc tgcaggtcat
      301 gcaggacgag atcttcgggc cgattcttcc ggtcaaaggg tacgccaacg ttgccgacac
      361 catcgactac atcaaccacc atccccggcc gctgggactg tactacttcg gcgacgaccg
      421 cgaagaggaa ggaaaggtcc tttcgcacac cacctcgggc ggggtgacgg tgaacgacgt
      481 ggttatgcac gtggctcagg aggatctgcc gttcggcggg gtggggccgt cgggcatggg
      541 cgcctaccat gggcacgacg gcttcaagac attcagccac gccaagtcga tcttcaccca
      601 gagcaaggtc aacgtggccg agctggccgg cctgcgtccg ccctacggcg agaagatgct
      661 caaggccatc cgcgcccaga tgcgttgagc gtaccttgga atccggcagc agcgagatcg
      721 accgcctcga gccgcgcgct tttgagcgcg acggcttcgt cgtggtgcgc ggctgctgcg
      781 acccggggct ggtgaggcgg attcgcgatc gcgcctcttc ctatctccag cccctgctgg
      841 ggcccgcaga gttcgaagct gacgtggggt atcccggatc ccctgcgagc cgcgcttcgg
      901 atgggggcga tacgtgccgc cgtctcctgc atgcctacgc tcgagattct gttttcaggg
      961 actgggccac aggaagctcc gtgggggccg ttctccgcgt tcttttcggg ggcgcggacg
     1021 tgatgatgtc gcaatgtcat cacaactgcg tgatgaccaa gcatcccggc tacagcagcg
     1081 cgacgctgtg gcatcaggac atccggtact ggtcgttcga ccgccccgag ctcgtctccg
     1141 tctggctcgc gctgggcgaa gagacggagg aaaacggcgc gctcaaggtc attccgggca
     1201 gccacgccat gaacatcgat cggggccggc tcgaccggga tcttttcctg cgccccgagc
     1261 tcgccgagaa cgccgagctc gttgagcatg cccagacggt cccgcttgaa gcaggcgacg
     1321 tgctgttttt tcactgccgg ttgttccacg cggcaggcaa gaatctcacg gacgaggtga
     1381 agctttccgt ggtgaccacg taccacaccg ccgacaaccg gccgatcccg gatacccggt
     1441 ctgcgcagta tccgggagtg ccgctgtagc cgaatcatgg gcgacgagga cgtctttcgc
     1501 gaggccatga gcgacgttgc gccgctgcag cgcaagcagc gcgcgcaacc cgagcgaggg
     1561 ccggtcgagg cgacctccgc gcagctcgaa cgccgcgaat tcgccctcgg caaccgggac
     1621 cgcgaggccg cggacccgaa ttacctgacc cttggcgagg tgccccagcg gcagccgcgg
     1681 gagattgtcg agtggaagaa ggacggtgtg cagaacgccg tcttcgcgcg gctgaaggcg
     1741 ggaaagtatc cggtggaggg ttctgtggat ctgcaccggc aaacggtgag ggaagcccga
     1801 gccgccgtgt tccggtttct caatctcgcc atggcgaagg gctggcgcat ggtgctcatc
     1861 gcccacggcc gcggcgagca gagcgagacc ccggcgcgta tcaaaagcta cgtggccgcc
     1921 tggcttgctc aggtgcccga ggtgatcgcc tatcacagcg cccagaacaa ccagggcggt
     1981 accggcgcct gctacgtgct gctgaagaag tcccctcagg cccgtgaaaa taatcgcgag
     2041 caatatggcg gcaaggggga agccgaggaa cccggctgaa gctgtgcccg cgggcgggca
     2101 ttttcagatc cggcctttgt ccaggtccag gcgccctcgc gccaggttca gtcgcagcac
     2161 gtcgctgcca ccctctgcca gccgcagcgc gcgcagccgt cggtagagct cggccagcgg
     2221 gtgggactcc accagcgcgc tcccgccaac cagctggatc ccggtgtcga cgatctcgcc
     2281 ggcggtttcc gttgcaaaca ctttgcaggc gatcccctcg ttgatcacgt tggacccgga
     2341 gtccgcgagc ctggccgtcc ggtaaaccat gctgcgcgcg gcatacgctt tgatgcgcag
     2401 atcggcgtag cgaagccgca cgccttcctt gctgccgcgc gcccggccgg agcggtcggg
     2461 cgccttgagg tgcgtcgtga gcagttcgag aacga
//
LOCUS       DBNHOX010000510         3414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_426341, whole genome shotgun sequence.
ACCESSION   DBNHOX010000510 DBNHOX010000000
VERSION     DBNHOX010000510.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_426341"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..425)
                     /locus_tag="AC16UZ_11160"
     CDS             complement(33..425)
                     /locus_tag="AC16UZ_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6247286.1"
                     /translation="MTKQTISTENAPAAIGPYSQAVRTGSLVFLSGQIPLVPATMDMV
                     AGDVIEQAHQVFRNLRAVASAAGGSLDDLVKVTVYLTDLNDFQSVNEVMTAYFSEPYP
                     ARAAIGVAALPRGAAVEVEGVMAHPGDG"
     gene            complement(468..2750)
                     /locus_tag="AC16UZ_11165"
     CDS             complement(468..2750)
                     /locus_tag="AC16UZ_11165"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR6247287.1"
                     /translation="MAKPARSDHQGSKHPGSRVVRKKTRAGEKPKLPQPESYFVGLLA
                     DESRRDDIAAPVSIESLTTLLSTYLEPDQVRRVERAHAYAINAHEGQFRRTGHPYISH
                     PLAVANILATMRMDHETIMAALLHDVIEDTDADRNALAQRFGKSVAEIVDGVSKLSKI
                     FRSRAEAQAENFQKMALAMAKDIRVIMVKLADRLHNMRTIGVMSREARQRIARETLDF
                     YAPIANRLGIHTMKVEFEELGFRALYPLRADRIGRAVRAARGNRKELVEELRKSLQDA
                     LDQESVTAEVIGREKHLYSIYRKMKAQHKPFAEIMDVFGFRVIVDEVEDCYRALGVVH
                     NTYKPVAGRFKDYIAIPKVNGYQSLHTTLFGMHGVPIEVQIRTRDMAAVADNGIAGHW
                     LYKSEGEDFQSSQQRARRWVKDLLDLQQRAGNPMEFVESLKIDLFPDEVYVFTPQGDI
                     LELPRGACAVDFAYAVHTDIGNRCVACRIDRNLAPLSQQLQSGQSVEIITSEGARPNP
                     DWLTFVVSSKARSGIRQALKAQQATDSIAFGRRLLNRSLAHANKSINDLDFRRLRRVF
                     TEFGVRRLDEVLARIGSGELMAYLVAQRLLAADDPDFQGVPVETGGPVAIGGGEGLVI
                     TYGRCCGPVPGDPIVGHMTPGKGFVVHMASCPNMQEVRRRRGNRELIPAYWANSPDNE
                     FLTTLRIQVNRKKGIIAELAAAVADADAGVENISVEERSAEVTSVIVNVSVTDRDTLA
                     RVMRKMRAIPSVLSIGRVSQ"
     gene            complement(2728..3000)
                     /gene="rpoZ"
                     /locus_tag="AC16UZ_11170"
     CDS             complement(2728..3000)
                     /gene="rpoZ"
                     /locus_tag="AC16UZ_11170"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR6247288.1"
                     /translation="MARITVEDCLENVENRFELVMLATRRARQMRRFGAEPLVPEEND
                     KPTVIALREIAEGLISNEMLDAQEASTEDEELEVSFGIGDGETGEI"
     gene            complement(3100..>3414)
                     /locus_tag="AC16UZ_11175"
     CDS             complement(3100..>3414)
                     /locus_tag="AC16UZ_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HIR6247289.1"
                     /translation="WQGARQVRAAYPDATSIFILPPSRAALVQRLEGRGLDAANVIEA
                     RSDKAREEISHYGEYDYLVVNDDFATALSELTQIIAASRLGLQPQRERLGALLKDLLR
                     QE"
BASE COUNT          557 a         1073 c         1115 g          669 t
ORIGIN      
        1 cacggcgcct gccggctcag ggctttgacg aatcagccgt caccggggtg agccatgacg
       61 ccttccacct ccacggccgc gccacgtggc agcgcggcca cgccgatcgc ggcgcgcgcg
      121 ggatacggct cgctgaaata ggctgtcatt acctcattga ccgactgaaa gtcgttgaga
      181 tccgtgaggt agacggtgac cttcaccaga tcgtccaggg acccgcctgc agcgctggca
      241 acagcgcgca gattcctgaa tacctgatgc gcctgctcga tcacgtcgcc cgcaaccatg
      301 tccatggtcg ctggaaccag cggaatctga ccggagagaa aaacgagact gcccgttctc
      361 accgcctgtg agtatgggcc gatggcggct ggggcgtttt ccgtgctgat ggtttgcttt
      421 gtcatgcggt ttccgttgtc acgcaggatg ttggccgaag acgggggtta ttggctcacc
      481 ctgcctatgg agagcacgct gggaatggca cgcatttttc gcatgacccg ggccagggtg
      541 tcgcggtcgg tgacggacac gttgacgatc acgctggtga cctcggcgct ccgctcctcc
      601 acggaaatgt tctccacgcc ggcatcggcg tcggctaccg cggcagccag ctccgcgatg
      661 atgcccttct tgcggttgac ctgaattcgc agggtggtca gaaactcgtt gtcgggtgag
      721 ttggcccagt acgcgggaat gagctccctg ttgccgcgtc gacggcgcac ctcctgcatg
      781 ttggggcagg aggccatgtg aactacgaac cccttccccg gtgtcatgtg cccgacgatg
      841 ggatctccgg gcacggggcc gcagcagcgg ccgtaggtga tgaccaggcc ctccccgccg
      901 ccgatggcaa ccgggccgcc ggtctctacc ggcactccct gaaagtcagg atcgtccgcg
      961 gcgagaaggc gctgggcgac gaggtaggcc atgagctcgc cgctgccgat cctcgccagc
     1021 acctcgtcga gccgacgcac gccgaattcc gtgaaaacgc gacgcaggcg ccggaagtcg
     1081 aggtcgttga tgctcttgtt ggcatgggcg agcgagcggt tgagcaggcg tcggccgaac
     1141 gcgatgctgt ccgttgcctg ctgggccttg agcgcctgac ggataccgct gcgggccttg
     1201 ctggacacca cgaaggttag ccagtcgggg ttgggtcgcg cgccttcgga agtgatgatc
     1261 tccacgctct ggccgctctg cagctgctgt gagagaggcg ccagattgcg gtcgatgcga
     1321 caggccacgc agcggttgcc gatgtccgtg tgaaccgcgt aggcgaaatc cacggcgcag
     1381 gcgccgcggg gcagctcgag aatgtcgccc tggggcgtga acacgtacac ctcgtcaggg
     1441 aacaggtcga tcttgaggct ttccacgaat tccatcgggt tgcccgcacg ctgctgcaga
     1501 tcgagcagat ccttgaccca gcgccgcgcc cgctgctggc tgctctggaa atcctcccct
     1561 tcggatttgt acagccagtg gccggcgatg ccgttgtcgg cgacggccgc catgtcccgc
     1621 gtgcgaatct gcacctcgat gggaacgccg tgcatgccga agagcgtggt gtgcagcgac
     1681 tggtaaccgt tcaccttcgg gatcgcgatg tagtccttga agcggccggc aaccggcttg
     1741 taggtgttgt gcaccacccc gagggcccga tagcagtcct ctacctcgtc cacgatcacg
     1801 cggaacccga acacgtccat gatctcggcg aacggcttgt gctgcgcctt catcttccgg
     1861 tagatggaat agaggtgctt ctcgcgaccg attacctccg ctgtgaccga ttcctggtcc
     1921 agcgcgtcct ggagggattt acgcagctct tcgaccagct ctttccggtt cccccgggcg
     1981 gcacgaacag cccggccgat tcggtcggcg cgcagcgggt acagggcccg gaaaccaagc
     2041 tcctcgaact ccaccttcat ggtgtgtatc ccgagccggt tggcgatggg cgcgtagaaa
     2101 tccagagtct cccgcgcgat gcgctgacgc gcctcccggg acatgacgcc gatggtgcgc
     2161 atgttgtgca gcctgtcggc cagcttcacc atgatcaccc ggatgtcctt ggccatggcc
     2221 agagccatct tctgaaagtt ttccgcctgg gcctcggctc gggagcggaa tatctttgac
     2281 agctttgaca ccccgtcgac gatctccgcc accgacttcc cgaaacgctg ggccagggcg
     2341 ttgcggtccg cgtcggtgtc ctcgatgacg tcgtggagca gagcggccat gatcgtctcg
     2401 tgatccatgc gcatcgtggc gaggatgttc gctaccgcca gggggtgcga gatgtaaggg
     2461 tgccccgtgc ggcggaactg cccttcgtgg gcgttgatgg cgtaggcgtg ggcgcgctcg
     2521 acgcggcgaa cctgatctgg ctccagataa gtggacagca gggtcgtcag ggattcgatc
     2581 gataccggcg cggcgatgtc gtcgcgcctc gactcgtcgg cgagcaggcc gacgaagtag
     2641 gattcgggtt gggggagctt cggtttttcg ccagcgcgtg ttttcttgcg gacaacgcga
     2701 gacccggggt gcttggagcc ctggtggtca gatctcgccg gtttcgccat ctcctatacc
     2761 gaaagaaacc tcaagttcct catcctccgt ggaggcttct tgtgcgtcaa gcatttcatt
     2821 tgatatgagc ccttcggcaa tttcgcgcag agcgatcacg gtgggcttgt cattttcttc
     2881 cggaaccagg ggctccgcgc cgaagcggcg catctggcgt gcccgccgcg ttgcgagcat
     2941 cacgagctcg aagcggtttt ctacgttttc caggcaatcc tcgacggtga tgcgggccat
     3001 ggcgaacctc tgatgcgatc ttctaaagtg gctccgagag agccggtgtg cagcgagcgc
     3061 gcgagtgtag ccgcccttcc ggaagattgt aaagtgccgt cactcctgcc ggaggaggtc
     3121 tttgagcagc gcgcccagcc gctcccgctg gggctgcagc ccgagccggc tcgccgcgat
     3181 gatctgcgtc agctcgctca gggctgtggc gaagtcgtcg ttcaccacca ggtagtcgta
     3241 ctccccgtag tgcgaaatct cctcccgggc tttgtcgctg cgcgcctcga tgacgttggc
     3301 ggcatccagg ccccgcccct ccagccgctg caccagggcg gcgcggctgg gcggcaggat
     3361 gaaaatgctg gtcgcgtccg ggtaagcggc gcgaacctgc cgggcaccct gcca
//
LOCUS       DBNHOX010000511         2141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1712352, whole genome shotgun sequence.
ACCESSION   DBNHOX010000511 DBNHOX010000000
VERSION     DBNHOX010000511.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1712352"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..572
                     /locus_tag="AC16UZ_11180"
     CDS             174..572
                     /locus_tag="AC16UZ_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247290.1"
                     /translation="MKFVGYLIGSVVILVVLIFVSQLVASESAEVVVLTSQGPDGGQD
                     TRLWVVDRDGLQYLRASPDSGWYQRLAAEPQVLLVRNGNSAAYRAEIRMALAEDVNRL
                     MREKYGWRDAYIEFLIGDRMAAVPVALIPD"
     gene            741..1760
                     /locus_tag="AC16UZ_11185"
     CDS             741..1760
                     /locus_tag="AC16UZ_11185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6247291.1"
                     /translation="MKDLKLGLQLGYWGAGPNPDMVKVAQEAERLGYDAIFTAEAWGS
                     DVFTPLAWIGAHTERIRLGTGIAQLSARAPTATAMAALTLDHLSKGRVILGLGVSGPQ
                     VVEGWYGRPFGKPLARTREYVSIIRQVLERKEPVTNDGEHYPLPYTGEGSWGLGKPLK
                     SITHPYRSDLPIFLGAEGPKNVAMTAEIADGWLPLYYSPYRQEVYADQIAHAKPGFEI
                     MQGVSVIINDDVEEALIPLKRNLALYIGGMGAKSRNFHNELVQRMGFADAAAKIQDLF
                     LSGRKDDAAAAVPTELADEISLVGSVERIKDRLQAWRETPVTSLLVSTQNISQLRTFA
                     EIVLG"
     gene            1869..>2141
                     /locus_tag="AC16UZ_11190"
     CDS             1869..>2141
                     /locus_tag="AC16UZ_11190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6247292.1"
                     /translation="MSDVKDEDRAAFFEAVDAACKKAVWCALASVAGDRPRVRIVHPT
                     WDGEVLWFATGPDSPKARQLRENPHVDIQYQVAPPDFVHVMVRGTAE"
BASE COUNT          384 a          651 c          721 g          385 t
ORIGIN      
        1 tggaagggtg aatagaatat ccacatcagc aggttccccc tcttcgctac cggaccgacc
       61 ctcacgtcac tggggcgatg atgcccgaac gttcagcctg gcgcagcagt tcatttcgcg
      121 gcagcgatcc gttagccttt ccggccacac ctggcgccgc gaggagggca ggggtgaaat
      181 tcgttgggta cctgatcggc tcggtggtca tcctggtcgt tctgatcttc gtctcccagc
      241 tcgtcgcgtc cgaatcggcg gaggtggttg tgttgaccag ccaggggccc gacggcgggc
      301 aggacacccg gttgtgggtg gtcgatcggg acgggctgca gtacctgcga gccagccctg
      361 actccggctg gtaccagcgc ctggcggcgg aaccgcaggt gctgctcgtc cgcaacggaa
      421 acagcgccgc ttaccgtgcc gagatcagaa tggccctcgc cgaggacgtc aatcgtctga
      481 tgcgggaaaa gtacggctgg cgggacgcgt acatcgagtt tctcatcggc gatcggatgg
      541 cagcggtgcc ggtcgcgctc attcccgact gatcgccggc cccgcgtagg cacggggccg
      601 gctttgagtt ctctgcatgt cctgggaacc ttgccgcgga gagattgagg cttgagcttt
      661 tgcttcccgc gtagtgtgat gccaaagcac gggaggtcgt cacgcggccg tgagttcagc
      721 caagcgaagg agcatttgag gtgaaggatc tgaaactggg cctgcaattg ggttactggg
      781 gcgctggacc caaccccgac atggtgaagg tcgctcagga agcagagcgt ctgggctacg
      841 atgcgatttt cacggctgag gcgtggggct cggacgtctt cacgccgctg gcctggatag
      901 gggctcacac ggagcggatc aggctcggga ccggcatcgc tcagctttcc gcccgggcgc
      961 ccaccgccac ggcgatggcc gccctgaccc tcgaccacct ttccaagggg cgcgtgattc
     1021 tcgggctcgg ggtgtcggga ccgcaggtcg tggagggctg gtacgggcgc ccgttcggaa
     1081 agcccctcgc gcgtacccgc gagtacgtga gcatcatccg ccaggtgctc gagcgcaagg
     1141 agccggtgac caacgacggg gagcactatc ctttaccgta cacgggtgag ggctcgtggg
     1201 gacttggcaa gccgctgaag tccatcaccc acccctaccg atccgatctg ccgatctttc
     1261 ttggcgcaga ggggccgaag aacgtggcca tgaccgcgga gatcgccgac ggctggctgc
     1321 ccctctacta ctcgccctat cggcaggagg tctacgccga ccagatcgcc catgccaagc
     1381 ccggcttcga gatcatgcag ggcgtctcgg tgatcatcaa cgacgacgtc gaggaagcgt
     1441 tgatcccgct gaagcgtaac ctggcgctgt acatcggcgg catgggcgcg aagtccagaa
     1501 acttccacaa cgagctggtg caacgcatgg gcttcgccga cgcggcggcc aagatccagg
     1561 atcttttcct ctccggtcgc aaggatgacg ccgcggcggc agttccgacg gagcttgccg
     1621 acgagatatc cctggtggga tccgtcgagc ggatcaagga ccggctgcag gcatggcggg
     1681 agacgccggt gacctcgctg ctggtctcca cccagaacat ctcgcagctg agaacgtttg
     1741 ccgaaatcgt gctcggttga gcgggtgaag ctcgccggcc gaatctcagg tgcgtggcag
     1801 gagaccaacg ttgggtccag cccctcgctg acaacacctt cgctgacaac aaatggggga
     1861 tcagctcgat gagcgacgtt aaggatgaag acagagcggc ctttttcgag gccgtcgatg
     1921 ccgcctgcaa gaaggcggtc tggtgcgcgc ttgcatcggt ggccggcgat cggccgagag
     1981 tgcgcatcgt ccatccaacc tgggatgggg aggtgctctg gttcgccacc ggtcccgact
     2041 cacccaaggc cagacagctg cgtgagaacc cgcacgtgga cattcagtac caggtggcgc
     2101 cgcccgattt cgtgcacgtg atggtgcgcg gaacggcaga g
//
LOCUS       DBNHOX010000512         5265 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_555142, whole genome shotgun sequence.
ACCESSION   DBNHOX010000512 DBNHOX010000000
VERSION     DBNHOX010000512.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_555142"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /gene="grxD"
                     /locus_tag="AC16UZ_11195"
     CDS             <1..279
                     /gene="grxD"
                     /locus_tag="AC16UZ_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HIR6247293.1"
                     /translation="PILLYMKGSPGAPQCGFSAQTVQCLVACGERFAYVDVLDNPEIR
                     AALPGYANWPTFPQLWVRGELVGGCDIITEMFQAGELEPLIKEAAETE"
     gene            complement(287..874)
                     /gene="rnt"
                     /locus_tag="AC16UZ_11200"
     CDS             complement(287..874)
                     /gene="rnt"
                     /locus_tag="AC16UZ_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HIR6247294.1"
                     /translation="MADRFRGFLPVVVDLETGGFDPGSHAILEIAAVQLRFDRGRIAP
                     DRKWHQAVEPFPGSRVEAASLKVTGIDLKDPNRQAAPENDALRGLFREVRRAVREAGC
                     TRAILVAHNAAFDQQFLNNALARQQIKRSPFHPFSFIDTASLAAVTYGHTVLSEACAR
                     AHIEFEPARAHNALYDAERTAELFCAIVNAWGRPR"
     gene            1043..2254
                     /locus_tag="AC16UZ_11205"
     CDS             1043..2254
                     /locus_tag="AC16UZ_11205"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR6247295.1"
                     /translation="MKIVLAYSGGLDTSVICRWLQETYGAEVVTFTADIGQGEEVEPA
                     RAKAQAMGVQEIFIEDLRDEFVRDYVYPMFRANTLYEGEYLLGTSIARPLIAKRLVEI
                     AAETGAEAVAHGATGKGNDQVRFELGAYALNPDIRVIAPWREWDLNSREALMAFCEQH
                     QIPVDYQRGAKSPYSMDANLLHISYEGDNLEDPWQEADADMWRWTVAPEEAPDEAQLV
                     EIDFLQGDPVAVDGEKLGPADLLARLNSLGGAHGIGRSDIVENRFVGMKSRGCYETPG
                     GTLLLKAHRAIESLTLDREVTHLKDELMPRYATLIYNGYWWSPERKVLQALIDESQKP
                     VSGRVRLKLYKGAVSVVGRMSQEESLYDANVVTFEDDGGAYHQADAAGFIRLNALRLR
                     LGARRGRKLSE"
     gene            2258..2824
                     /locus_tag="AC16UZ_11210"
     CDS             2258..2824
                     /locus_tag="AC16UZ_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247296.1"
                     /translation="MNNYQVLFSGEVSPQANRDIVRAKLACELDIDDRKAAQLFSGRT
                     VVIKSQLSHRQAMELQERLASLGAICRVKDLTPKTRNNVADFRVDRSGKSDATLRDIT
                     AAHTECPRCGHMQLEAAHCTRCGVDIAAAVRQRQKEDLIIEKKIRELRAKQDGLLGRA
                     GAAPGKALQGAASSTVKTRGIGWFRKSS"
     gene            complement(2857..3498)
                     /gene="nth"
                     /locus_tag="AC16UZ_11215"
     CDS             complement(2857..3498)
                     /gene="nth"
                     /locus_tag="AC16UZ_11215"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR6247297.1"
                     /translation="MNNDKRRAIFERLRAANPAPTTELDYRNPFELLIAVILSAQATD
                     VSVNKATGPLFEAAGTPTEMKALGLRKLKSYIKTIGLFNTKAANILKTCEMLLTQHGG
                     EVPEDRQALEALPGVGRKTANVVLNTAFGHPTIAVDTHIFRVCNRTRIAPGKNVNQVE
                     QKLLKVVPDEFKRDAHHWLILHGRYTCIARKPRCGSCVIEDLCEFRQKTDLEG"
     gene            complement(3495..4160)
                     /gene="rsxE"
                     /locus_tag="AC16UZ_11220"
     CDS             complement(3495..4160)
                     /gene="rsxE"
                     /locus_tag="AC16UZ_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HIR6247298.1"
                     /translation="MSVAKPFRNAAIASNPAWAQLLGLCPLLAVSTSLVNALALGAAS
                     AFVLLGSNITIAAIRRWIPELARLPSFVLVIATFTTCAMLLMQAYAFELYLRIALFVQ
                     IIVTNCMILGRVEAFASRQPVGRAFLDAAGTACGFALALLAMGAARELIGHGSLMAGM
                     EQLIPGSESWRLNLSESGRGLLLASLPPGAFIIAGLLLGAGNAILRRPNPAPSGSAGE
                     NSA"
     gene            complement(4157..4633)
                     /locus_tag="AC16UZ_11225"
     CDS             complement(4157..4633)
                     /locus_tag="AC16UZ_11225"
                     /inference="COORDINATES: protein motif:HMM:NF016121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR6247299.1"
                     /translation="MLIAAACAMLLALTDAWTADRIARNARSHELSQITDLAGAAPPA
                     DTTWNEDVWDFCSGTKLARSSAAGYGGPIRLVVGVSQGRILGIRVTAHQETPGLADFL
                     REPKRGWLAALNGRTGPETAAVDAVAGATITSEAVVRAVRDAMDRAQDASAEGCRA"
     gene            complement(4638..>5265)
                     /locus_tag="AC16UZ_11230"
     CDS             complement(4638..>5265)
                     /locus_tag="AC16UZ_11230"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR6247300.1"
                     /translation="ALAKHLYGGLGSNLFNPALVGYAVVLVSFPVAMASWPIPMDGET
                     GATALVSMKYREGLTVAEAWQGARGFGAIGGYGWEWINLGFLLGGAVLVWRRLAAWRV
                     PATILATIAVLAAAFWDGGSSTSSGSPLFHWFTGSTMLTVFFFATDPVTHPVSPRGQI
                     VFGIVVGTATFLIRAFGNYPDGAAFAILLGNALTPSLDKRLQEASANA"
BASE COUNT          941 a         1671 c         1788 g          865 t
ORIGIN      
        1 ccgattctgc tttacatgaa gggcagcccg ggcgcaccgc agtgcggatt ttctgcccag
       61 acggttcagt gcctggtcgc ctgcggagag cgatttgcct acgtggacgt cctcgacaac
      121 ccggaaatca gagccgcgct gccgggctac gcgaactggc cgacctttcc ccagctctgg
      181 gtgcgcggcg agctcgtcgg aggctgcgac atcatcaccg agatgttcca ggcgggtgag
      241 ctcgagcctc tgatcaaaga agccgccgag acggagtgac gatcgttcaa cgcggacgcc
      301 cccaggcgtt cacgatggcg cagaaaagct cggccgttcg ctcggcgtcg tagagcgcgt
      361 tgtgtgcccg ggccggctcg aactcgatgt gcgcacgcgc gcaggcttcg ctgagcaccg
      421 tgtgaccgta agtcaccgcg gccagagacg cggtgtcgat gaagctgaac ggatggaaag
      481 ggctgcgctt gatctgctgg cgcgcaaggg cattgttgag aaactgctga tcgaacgcgg
      541 cgttgtgcgc gaccagaatg gcacgcgtgc agccggcttc tcgcacggcc cggcgcactt
      601 cccggaaaag acccctcaac gcatcgttct ccggtgccgc ctgccggttg ggatccttca
      661 ggtcgatgcc ggtcaccttc aacgaagcgg cctccacccg gctgcccggg aagggctcca
      721 cggcctgatg ccacttccgg tccggcgcga tccgcccgcg atcgaaacgc agctgcacgg
      781 ctgcgatctc cagaatggcg tgggagccgg gatcgaagcc tcccgtctcc agatcgacca
      841 ccaccggcag aaaaccccga aaacgatccg ccatctccat gcgggcacat taacacggct
      901 ttgacgcgac tttgcgcgcc ggaagcggga aggcacccgg ccggaagggt ttcccgcggg
      961 acggcggctg gcgggccctg ctcaagttac ttaagattgc gacccttttt tctcggcgac
     1021 cgcactcggg ggaaacgtga cgatgaagat tgtgctggca tactcaggtg gcctggatac
     1081 ctccgtgatc tgccgctggt tgcaggaaac ctatggtgcc gaggtggtga cgttcaccgc
     1141 ggacatcggc cagggtgagg aggtggagcc tgccagggcg aaggcccagg ccatgggcgt
     1201 acaggagatc ttcatcgagg atctgcgcga cgagttcgtg cgcgactacg tctacccgat
     1261 gttccgcgca aatactctct acgaaggcga gtacctgctc gggacgagca ttgcgcggcc
     1321 tctgattgcc aagcgtctcg tcgagatcgc cgctgaaacc ggcgcggagg ccgtcgctca
     1381 cggagcaacc ggcaagggca acgaccaggt ccgcttcgag ctcggagcat acgcgctgaa
     1441 tccggacatt cgggtgatcg cgccgtggcg cgagtgggat ctcaattccc gggaggcgtt
     1501 gatggcgttc tgcgagcagc accagatccc cgtggattat cagcgcggcg ccaagtctcc
     1561 ttactccatg gatgccaacc tgctccacat ctcctacgaa ggcgacaacc tagaagatcc
     1621 ctggcaggag gcggatgccg acatgtggcg ctggacggtg gcgcccgagg aggcgccgga
     1681 cgaggcgcag ctcgtcgaga tcgatttcct gcagggcgat cccgtggccg tcgacggcga
     1741 gaagctcgga ccggccgatc tgctcgccag gctcaattcc ctggggggcg cccacggtat
     1801 cggtcgcagc gacatcgtgg agaaccgctt cgtcgggatg aagtcccgag gctgctacga
     1861 gacaccgggg ggaactctgc tgctcaaggc ccaccgcgcc atcgagtccc tcacgctgga
     1921 ccgggaagtc acccatctca aggacgagct catgccccgg tacgcgacgc tcatctacaa
     1981 cggctactgg tggtctcctg agagaaaagt cctccaagcg ctgatagacg aatcacagaa
     2041 acccgtctcg ggtcgggtta ggctcaagtt gtacaagggt gcggtatccg tcgtgggacg
     2101 gatgtcgcag gaggagagcc tgtacgatgc gaacgtcgtc actttcgagg acgacggggg
     2161 ggcgtaccat caggccgacg cggcgggttt catccgcctt aacgccctcc gcctgcgctt
     2221 gggcgcaagg agaggacgaa agctttcgga gtaatcgatg aacaactatc aagtgctgtt
     2281 cagcggtgag gtttccccgc aggcgaatag ggacatcgtg cgggcaaagc tggcctgcga
     2341 actcgacatc gacgaccgca aagccgcgca gcttttcagc ggccggacgg tggtcatcaa
     2401 gagtcaactc agccatcggc aggccatgga gctgcaggag cgtctagcca gcctgggcgc
     2461 catatgccgg gtcaaagacc tcacgccgaa gacccgcaac aacgtggcgg acttccgggt
     2521 ggacaggtcc ggtaaatccg acgcgaccct gcgcgacatc accgctgctc acacggagtg
     2581 cccgcgctgc gggcacatgc agctggaagc cgctcactgc acgcgctgcg gggtcgacat
     2641 cgcggcagcc gtccggcagc ggcagaaaga ggacctgatc atcgagaaga agatacgcga
     2701 gctgcgcgcc aagcaggacg gtctgctcgg gcgagcgggc gccgcgcccg gaaaggcgct
     2761 gcagggcgcg gcttcgtcga cggtgaaaac tcgcggcatc ggctggttca gaaagtcgtc
     2821 ctgagtgccc gcctgcgcgg ccgcgccgcg gatacctcag ccctcgagat cggtcttttg
     2881 acgaaactcg cagagatcct cgatcacgca tgatccgcag cgcggcttgc gcgcgatgca
     2941 ggtatagcgg ccgtgcagta tcagccagtg gtgcgcgtcg cgcttgaact cgtccggcac
     3001 gaccttgagc agcttctgct ccacctgatt gacgttcttg cccggcgcga tccgggtgcg
     3061 gttgcagacc ctgaagatgt gggtgtccac ggcgatggtg gggtgcccga acgccgtgtt
     3121 gagaaccacg ttggcggttt tgcgtcccac cccggggagc gcttccaggg cttggcgatc
     3181 ctccgggacc tcgccgccgt gctgcgtgag cagcatctcg caggtcttca agatgttggc
     3241 cgccttggta ttgaacaagc cgatggtctt gatgtagctc ttgagctttc gcagcccgag
     3301 cgccttcatc tcggtcggcg ttccggccgc ctcgaacagc gggcccgtgg ctttgttgac
     3361 gctgacgtcg gtggcctggg cggagaggat gacggcgatc agcagctcga aggggttgcg
     3421 gtagtcgagc tctgtcgtcg gcgccgggtt ggccgcccgt aggcgctcga agatggcgcg
     3481 gcgtttgtcg ttgttcacgc tgagttttct cctgcggagc cggatggggc tgggttcggt
     3541 cggcgcagaa tcgcgttgcc cgcaccgagg agcaggccgg cgatgatgaa agcgccgggc
     3601 ggcaggctcg ccaggagcag accgcgtccg ctctccgaca ggttcaaccg ccatgactcc
     3661 gagccgggaa tcagctgctc cattcccgcc atcagggagc cgtggccgat gagctctcgg
     3721 gcggcgccca tggcgagcag ggccagcgcg aacccgcaag ccgtgcccgc cgcgtccaag
     3781 aaagcgcgcc ccaccggctg cctgctcgcg aacgcctcga cccggccgag gatcatgcag
     3841 ttggtgacga ttatctggac gaacagcgcg attcgcagat acagctcgaa ggcgtaggcc
     3901 tgcatcagga gcatcgcgca cgtcgtgaac gtggcgatga cgagcacgaa actcggaaga
     3961 cgggcgagct ccggtatcca tcggcggatg gcggcgatgg tgatgttgct gccgagaagc
     4021 acgaacgcgc ttgccgcgcc cagcgccagg gcgttgacca ggctggtgct gacagcgagc
     4081 agggggcaga ggcccagcag ctgcgcccag gccgggttgc tggcgatggc ggcgttgcgg
     4141 aacggcttgg cgacgctcat gcgcggcatc cctcggcgct ggcgtcctgc gcccggtcca
     4201 tggcgtcgcg aaccgcgcgg acgaccgctt cgctggtgat cgtggccccc gctaccgcat
     4261 cgacggccgc ggtttccggt ccggtccggc cattcaatgc ggcaagccaa ccgcgcttgg
     4321 gctcgcgcag gaagtcggca aggccgggcg tttcctggtg cgcggtgact ctgatgccca
     4381 ggatgcgccc ttgcgacacg ccgacgacca accggatcgg gccgccgtaa cccgccgccg
     4441 agctgcgggc gagtttcgtg ccgctgcaga aatcccagac gtcctcattc caggtcgtat
     4501 cggcaggcgg cgctgctccc gccaggtcgg tgatttggct cagctcgtgg ctgcgggcgt
     4561 tccgggcgat gcgatccgcc gtccaagcat cggtgagggc gagcagcatc gcgcaggcgg
     4621 ccgcgatgag cacgagctta tgcatttgcc gacgcctcct gcagacgctt gtcgagagag
     4681 ggcgtgaggg cgttgccgag gaggatggcg aatgccgcgc cgtccggata gttaccgaag
     4741 gcccgaatca agaaggtcgc ggtgcccacc acgatgccga agacgatctg accgcgcggc
     4801 gaaaccgggt gcgtaacggg gtccgttgcg aagaagaaga cggtcagcat cgtggagccg
     4861 gtgaaccagt ggaagagcgg ggacccgctg ctggtcgagc tgccgccgtc ccagaacgcg
     4921 gccgccagca cggcgatggt ggccagaatg gtcgccggca cgcgccatgc cgccagccgc
     4981 cgccacacga gcacagcccc gcctaggagg aagcccaggt tgatccactc ccagccgtag
     5041 ccgccgatgg cgccgaagcc gcgtgcgccc tgccacgctt ccgcgacggt gagcccctcc
     5101 cggtacttca tcgacaccag cgccgtggcg ccggtttcgc cgtccatcgg gatgggccag
     5161 ctcgccatcg ccacgggaaa cgagaccagg acgacggcgt accccacgag cgcggggttg
     5221 aacaggttgc tccccagccc gccgtaaagg tgttttgcca gggct
//
LOCUS       DBNHOX010000513         2127 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_555191, whole genome shotgun sequence.
ACCESSION   DBNHOX010000513 DBNHOX010000000
VERSION     DBNHOX010000513.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_555191"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="AC16UZ_11235"
     CDS             complement(<1..665)
                     /locus_tag="AC16UZ_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6247301.1"
                     /translation="MTHLMNNYARLPVAFARGSGAYLEDTSGKQYLDALSGIAVCGLG
                     HAHPRVTRAIAEQAETLLHTSNLYEVPLQERLADRLCEISGMDRVFFGNSGAEANEAA
                     IKIARLYGHKRGEKRPTIVVVDNSFHGRTMATLTATGNRKVQAGFEPLLAGFVRAPFD
                     DLDSVREIAGNNGDVVAVMVEPILGEGGIILPNDDYLPGLREICDQHEWLLILDEVQT
                     GNGR"
     gene            complement(775..1953)
                     /locus_tag="AC16UZ_11240"
     CDS             complement(775..1953)
                     /locus_tag="AC16UZ_11240"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR6247302.1"
                     /translation="MPTETGTSEFDVEIDRTNTSSSKWDKYAGRDVLPFWVADMDFAM
                     PSFIRAAIAERLAHPVLGYTRTPDAAVQAFQNWLERCYGWEVPEEWLVWIPGVVTGFN
                     LAARAVAEPGGSVLIPTPVYYPFLSTPRHAGQDSIEVPLVRDGERWAMDFDAMSAACR
                     NGTRLFMLSNPQNPTGRAYTEEELSELAAFCLRHDLYLCSDEIHASIILAPEARHIPI
                     AAIAPEIAERTISLYAATKTYNIPGLGCAVAVIADARLRRDFRRAMGGLVPGIGPLAY
                     AASTAAFADASDWTTRLIDYLRGNHQRVREVVGERMTPVEATYLAWIDLTDLGFADPA
                     ARLEAFGIGLSEGADFGGAGYVRFNFACPRSLLDRGLARFRDALARLDAAQRAGASPD
                     "
BASE COUNT          373 a          668 c          716 g          370 t
ORIGIN      
        1 cggccgttgc ccgtctggac ctcgtccagg atgagcagcc actcatgctg gtcgcagatc
       61 tctcgcagcc cgggaagata gtcgtcgttc ggcaggatga tgcccccctc gccgagaatg
      121 ggctccacca tcacggcgac cacgtcgccg ttgttgcccg cgatctcgcg aaccgagtcc
      181 aggtcgtcga aaggagcgcg gacgaatccg gccagcaggg gctcgaagcc cgcctgcacc
      241 ttgcggtttc ccgtcgccgt cagcgttgcc atggtgcggc cgtgaaagct gttgtccacg
      301 accacgatgg tgggtcgctt ttctccccgc ttgtggccgt agagccgggc gatcttgatg
      361 gccgcctcgt ttgcctcggc gccggaattg ccgaagaaga cccggtccat gcctgagatc
      421 tcgcagagcc gatcggccag ccgctcctgc aggggaacct cgtagaggtt ggaagtgtgg
      481 agaagcgttt ccgcctgctc ggcgatggcg cgcgtgaccc ggggatgggc gtgccccagg
      541 ccgcagacgg cgatgccgct gagagcgtcc agatactgct ttccggacgt gtcctcgaga
      601 tacgcgccgc taccccgggc gaacgccacg ggtaggcggg catagttgtt catgaggtgc
      661 gtcatgtgct gcctgcggat cgtcgtaggg aagattttcg cctccccaaa accattccag
      721 gcagctgttg ctgcccggaa aaacgctgaa tcgtactgca ggggagtggt cagttcaatc
      781 gggcgacgca cccgccctct gcgctgcgtc caaccgggcg agcgcatctc gaaagcgtgc
      841 caggccgcgg tcgagcaggg atcgagggca ggcgaagttg aaacgcacgt acccggcccc
      901 gccgaagtcc gccccttcgg aaagaccaat gccgaatgct tccaggcgag cggcgggatc
      961 ggcaaagccg agatcggtga gatcgatcca ggcgagatag gtggcctcga cgggcgtcat
     1021 tcgctcgccg accacctcgc gcacgcgctg gtgattgccg cgcaggtagt cgatcaggcg
     1081 cgtcgtccaa tcgctggcgt ctgcaaaggc agcggttgaa gccgcgtagg ccagcggtcc
     1141 tatacccggg acgagccccc ccatggcccg acggaagtcg cgtcgcaggc gcgcatcggc
     1201 gatcaccgcc accgcgcagc cgaggccggg aatgttgtag gtcttggtgg cggcgtaaag
     1261 gctgatggtg cgctcggcga tttccggagc gatggcggca atgggaatgt gacgagcctc
     1321 gggcgccaga atgatgcttg cgtgaatctc gtccgagcat aggtagaggt cgtggcgcag
     1381 gcagaacgcc gcgagctcgg acagctcctc ctcggtatac gcgcggcccg tcgggttctg
     1441 cgggttgctc agcatgaaca gccgtgtccc gttgcggcaa gcggcgctca tggcgtcgaa
     1501 gtccatggcc catcgctcgc cgtctctcac gagcgggacc tcgatggagt cctggcctgc
     1561 atggcggggg gtcgacagaa acgggtagta gacgggtgtg ggaatgagaa cggagccccc
     1621 gggctcggcc accgcgcgcg cggccaggtt gaatcctgtc accaccccgg gtatccagac
     1681 cagccactcc tcgggcactt cccagccata gcagcgctcg agccagttct ggaacgcctg
     1741 caccgcagcg tcaggagtgc gcgtgtatcc cagcacggga tgcgcgaggc gctccgcgat
     1801 ggccgcgcgg atgaatgacg gcatggcgaa atccatgtcc gcgacccaga aagggagcac
     1861 gtcgcggcct gcgtacttgt cccatttgga gctcgacgtg ttggtgcgat cgatctcgac
     1921 atcgaactcg ctcgttcccg tttcggtcgg catgttccct ccgtgcaccg atccgcgcct
     1981 tgatggactg cgctccggct ccctgttgcg cggcctgatc ggagggtaga atgtcggcat
     2041 tccatgccaa gcgcaattcg cgtcgagggt tgacgagcaa tgagcgaaga agtgatcgcc
     2101 accatagagg agcagatcgc gacacat
//
LOCUS       DBNHOX010000514         4191 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_234346, whole genome shotgun sequence.
ACCESSION   DBNHOX010000514 DBNHOX010000000
VERSION     DBNHOX010000514.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_234346"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1584
                     /locus_tag="AC16UZ_11245"
     CDS             133..1584
                     /locus_tag="AC16UZ_11245"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR6247303.1"
                     /translation="MRLEDRGLLRTAAYVAGEWVAADSGETFDVRNPATGEVVAAVAR
                     CGAAETRRAIEAAERAQVDWRARTAKERARVLRRLYELMMLHQEDLARILTAEMGKPI
                     VEARGEIAYGASYLEWFAEEAKRVYGDTIPGPAPDKRIVTIKQPVGVVGCITPWNFPN
                     AMLTRKVGPALAAGCAVVCKPATATPLSALAIAELGARAGVPAGVLNVLAGSTREIGS
                     EITGNPAVRKLTFTGSTGVGKALIEQCAATVKRTSMELGGNAPFIVFDDADVDAAVAG
                     AIASKYRNAGQTCVCANRLLVQAGVYEEFAEKLSAAVAEFRLGDGAEETTTIGPLIDA
                     GAADEVLAMIDDAIANGASAVIGGARAAQGGSFVQPTVLTEVTPRMRVFREEIFGPVA
                     PLIRFETEDEAIRLANDTEFGLASYFYARDVGRIWRVAEGLEYGIVGINEGIVSNEMA
                     PFGGVKESGNGREGSKYGMDDYLEIKYLCMGGI"
     gene            1599..3740
                     /locus_tag="AC16UZ_11250"
     CDS             1599..3740
                     /locus_tag="AC16UZ_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247304.1"
                     /translation="MWFHEKAKHRPVSLGPYPLETLPRDPAQSEIELRMPLARQLPLE
                     ESASELGAATGRYLSLFLEFRDGEVAPQPAPVPDDVDLRADDVKGLAYFFDADHVGIC
                     ELPSGARLIESDALDHAMAVVILVRFGRQPEADNLARDWIAGAEHDVAELRAAEIAVT
                     VAGYIRQLGYPATAHVRRATTVDLDRLAVLSGVCVRRDGAVCSPYFGDGFAAAAVTTD
                     YALAVDAPLAPDQGKARGRRYHRGQNGAVSGRERRRRDRRASHLSRYPMEQVRRVERP
                     TTLIIDDEVPRVPKRANFFTRAAFGDLGEKSQREVRRFAYKHPSTAGTMQLIRSMVSL
                     QDGEIAGSRWASLDSKANAAAIKSLGYHLGADLVGICEIPPYAWYSHQADGTLIEPYH
                     RFAVVMLIDQGFDTMEGASGDDWISGAQSMRGYVRGAEIAGVMAEFLRSVGVSARPQT
                     NADSDVLHIPLILWAGLGELSRIGELVLNPFVGPRFKSVVLTTDLPLEVDAPIDFGLQ
                     YFCNHCLKCARECPCNAIPTGSKVMFNGYEIWKPDVERCTRYRITNQKGAACGRCMKT
                     CPLNKVVGADGALAHRAGNWLAIHLPWLRPLLVPIATRLDDRTGYGARNPVKKWWFDL
                     EIVDGVCVHAEANQRDLDVGRSAASRRDTVGYHPADTMPAPDAQGPVPVNHREAVGRS
                     ALLETPADARRRKRKGEPGPSHYVPLVDVTD"
     gene            3841..>4191
                     /locus_tag="AC16UZ_11255"
     CDS             3841..>4191
                     /locus_tag="AC16UZ_11255"
                     /inference="COORDINATES: protein motif:HMM:NF047172.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HIR6247305.1"
                     /translation="MNDFDRSLPMLLYRALDLIMPRFRAIFGGFGLTEQQWRVLRVLW
                     QRDGRALLELADATLISAPSMVGVVDRLARSGLVERRRSQSDRRVVRVFLTGRGRDLE
                     EQVTPLVDAAYADLR"
BASE COUNT          698 a         1313 c         1446 g          734 t
ORIGIN      
        1 cgagctaaat tagataacaa gtaatttgca taaaaacatg tcgaagattc gttgctctgt
       61 ctcacaccgg cgccaggcgg ccaggtcggc gttagaatgg gcgggtattt caccggcggg
      121 caggggatag gcgtgcgtct agaggacagg ggtctgctgc gaaccgcagc ttacgtggcc
      181 ggcgagtggg tggcggcaga cagcggcgaa accttcgacg ttcgcaatcc ggcaaccgga
      241 gaagtggttg ccgcggtggc gcggtgcggc gccgcggaga cccgccgtgc catcgaggcg
      301 gcggagcgtg ctcaggtgga ctggcgggcg agaacggcca aggagcgtgc gcgcgtgctg
      361 cgccggcttt acgagttgat gatgctgcat caggaggatc ttgcgcggat tcttaccgcc
      421 gagatgggca agcccatcgt tgaagcgcgc ggcgagatcg cctacggggc aagctatctg
      481 gaatggttcg ccgaagaggc caagcgcgtt tacggcgaca ccatcccggg ccccgcgccg
      541 gacaagcgga tcgtcaccat caagcagccc gtgggcgtcg tcggctgcat caccccgtgg
      601 aatttcccca atgccatgct gacccgcaag gtcgggcccg ccctcgccgc cggctgcgcg
      661 gtggtgtgca agccggccac cgcgacgccg ctgtctgcac tggccatcgc ggagcttggg
      721 gcgcgggccg gcgtgccggc gggtgttctg aacgtgctgg ccggaagcac ccgggaaatt
      781 ggcagcgaga tcaccggcaa cccggcggtg cgcaagctga ccttcaccgg atccaccggc
      841 gtgggcaaag cgctcatcga gcagtgcgcg gccacggtca agcgcacgtc catggagctc
      901 ggggggaacg cgcccttcat cgtcttcgac gatgcggacg tggacgccgc cgtcgcgggg
      961 gccatcgcat ccaagtaccg aaacgcgggc cagacgtgcg tgtgcgccaa ccgcctgctc
     1021 gtccaggccg gggtatacga ggaatttgcg gagaagctct cggcggcggt ggcggagttc
     1081 cggctgggcg acggggcaga ggagaccacc accatcggtc ccctcatcga tgcaggggct
     1141 gccgacgagg tgctggcaat gatcgacgac gcgatcgcca acggcgccag cgcggtgatc
     1201 ggcggggcgc gggccgccca ggggggctcc ttcgtccagc caaccgttct cacggaagtg
     1261 accccgcgca tgcgggtgtt tcgcgaagag atcttcgggc cggtggcgcc gctcatccgg
     1321 ttcgagacgg aggacgaagc gatccggctg gccaacgata ccgagttcgg gctggcgtcg
     1381 tatttctacg cccgggacgt gggtcgaatc tggcgcgtcg ccgagggact cgaatacggt
     1441 atcgtcggga tcaacgaggg catcgtctcg aacgagatgg cgcctttcgg cggggtcaag
     1501 gagtcgggta acggccgcga gggatcgaag tacgggatgg acgactatct ggagatcaaa
     1561 tatctctgca tgggcgggat ctagcggggc tcgccgtcat gtggtttcac gagaaggcaa
     1621 agcatcggcc cgtgagcctc ggcccctatc ccctggagac gttgccccgg gatccggctc
     1681 agagcgagat cgagctgcgc atgccgctgg caaggcagct gccgctggaa gagtcggcgt
     1741 ctgagcttgg cgcggccacc gggcgctacc tctcgctctt cctggagttt cgcgacggcg
     1801 aggtggctcc tcagcccgcg cccgtccccg acgacgtcga cctccgtgcg gacgacgtca
     1861 aaggtctggc ctacttcttc gatgcggatc acgtgggtat ctgcgagctc ccatcgggcg
     1921 cacgactgat tgaatctgat gccctggatc acgccatggc cgtcgtcatc ctcgttcgct
     1981 tcgggcgcca gccggaagcc gacaacctcg cccgggactg gattgcgggc gccgagcacg
     2041 atgtggcgga gctcagagcg gcggagatcg ccgtcacggt ggccggctac atccggcagc
     2101 tgggctaccc ggccaccgct cacgtccggc gggccaccac cgtggacctg gaccggctgg
     2161 ccgtcctgtc cggcgtctgc gtgcgcaggg acggtgccgt ctgctccccg tactttggtg
     2221 acggcttcgc tgcagccgcg gtgaccacgg attacgccct ggcggtggat gcccctctgg
     2281 ccccggacca gggcaaggcc cggggccggc gctaccaccg gggtcaaaac ggcgcggtct
     2341 ccggcagaga acgccgtcgc cgggaccggc gtgcgtctca cttgagccgc tatcccatgg
     2401 agcaggtccg tcgggtcgaa cggccgacca cgctgatcat cgacgatgag gttcccaggg
     2461 ttccgaagcg ggcgaacttt tttacgcggg cggcttttgg cgaccttggc gagaagtccc
     2521 agcgcgaggt ccgccggttt gcctacaagc acccttccac ggcggggacc atgcagctca
     2581 tcaggagcat ggtgtcgttg caggacgggg agatcgccgg ttcccgttgg gcctcgctgg
     2641 actcgaaagc caacgctgcg gccataaagt cgctcggcta ccacctcggg gcggatctcg
     2701 tgggcatctg cgagattccg ccgtatgcct ggtactccca tcaggcagac ggaacgctca
     2761 tcgagccgta tcaccgcttt gctgtggtga tgctcatcga tcagggcttc gacaccatgg
     2821 agggcgccag cggggacgac tggatttccg gtgcgcagtc catgcgaggc tacgtgcgtg
     2881 gtgcggaaat cgctggcgtg atggccgagt ttctgcgctc cgtgggagtc tcggcgcgac
     2941 cgcagaccaa cgcggacagc gacgttctgc acataccgct gattctgtgg gcgggccttg
     3001 gcgagctcag ccgaatcggc gagctggtgc tgaacccgtt cgtcggtccc cgtttcaagt
     3061 ccgtggtgct gaccacggat ctgcccctgg aggtggacgc gcccatagac ttcgggctgc
     3121 agtatttctg caatcattgc ctgaagtgcg cgcgggaatg tccctgcaac gcgattccta
     3181 cgggctccaa ggtcatgttc aacgggtacg agatctggaa gccggacgtg gagcggtgca
     3241 cgcgctaccg cattaccaac cagaaagggg cggcctgcgg aaggtgcatg aagacctgcc
     3301 cgctcaacaa ggtggtgggc gccgatggcg ctcttgcgca ccgagccggc aactggctgg
     3361 caattcatct tccctggctg cggccgctcc tggttcccat cgccacgcgg ctggatgacc
     3421 ggaccggcta cggggcccgt aacccggtga agaagtggtg gttcgatctg gagatcgtcg
     3481 acggcgtatg cgtccatgca gaagcgaacc agcgcgacct cgacgtcggt cggtcggcgg
     3541 cttcccggcg cgataccgtg ggctaccatc cggcggatac gatgccggct cccgatgctc
     3601 agggtcccgt tcccgtcaac caccgggaag ccgttggccg gagcgccttg ctggaaaccc
     3661 ctgccgatgc gcgtcgccgg aaacgcaaag gcgagcctgg cccctctcat tacgttccgc
     3721 tcgtcgacgt caccgattga ccccaccgct tttcggcccc cttgcagccg atgattaccc
     3781 ggttgacgcg tctcgagatt taactatcat tttaattatt aatacatgaa ctaaaagcgc
     3841 gtgaacgact tcgaccgatc cctgcccatg ctgctttacc gggcccttga cctgatcatg
     3901 cccagatttc gcgccatatt cggcgggttc gggctcactg agcagcagtg gcgcgtgctc
     3961 cgggtgctgt ggcagcggga tggtcgtgcg ctgctcgaac ttgccgacgc caccctgatc
     4021 tcggcgccca gcatggtcgg ggtggtggat cgcctggcgc gcagcggtct ggtggaacga
     4081 cgccggtcgc agagtgatcg gcgggtcgtg cgggtgtttc tcaccgggcg cggcagggac
     4141 ttagaggaac aggtcacgcc cctggtggac gcggcctacg cggacctgcg c
//
LOCUS       DBNHOX010000515         1560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_298936, whole genome shotgun sequence.
ACCESSION   DBNHOX010000515 DBNHOX010000000
VERSION     DBNHOX010000515.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_298936"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="AC16UZ_11260"
     CDS             complement(<1..321)
                     /locus_tag="AC16UZ_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247306.1"
                     /translation="MSDDEDFHSAMSDVAPLDQKRRRKAEHQEAQLEAKKEMPRELSL
                     SQLEHRYSALGINRSPDVDPNPLTLGEVPQVAPRDMLEWKKDGVQNAVFSRLKQGRYP
                     VEGSL"
     gene            complement(323..1114)
                     /locus_tag="AC16UZ_11265"
     CDS             complement(323..1114)
                     /locus_tag="AC16UZ_11265"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6247307.1"
                     /translation="MSALAPLEPLAFARDGYLVVRGLCPETLRSRLLEVSRQHLSPVL
                     GPVEYEADLGYPGAPANRMAEGGNTVRRLLNAYTRDQAYRDWATGLAVGDRLRALFAQ
                     VHGRPFGPDDVRVSQCHHNCVMTKHPGYSSATLWHQDIRYWSFDRPELISVWLALGAE
                     SARNGGLTVIPGSHRLSLDRGRLDGALFLRTDLEENQAMIASAVPVELQPGDVLFFHC
                     RLFHAAGMNLADQAKLSLVFTYHAAGNRPIPETRSASLPGIPLRA"
     gene            complement(1161..>1560)
                     /locus_tag="AC16UZ_11270"
     CDS             complement(1161..>1560)
                     /locus_tag="AC16UZ_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6247308.1"
                     /translation="MTIMQDEIFGPLLPVKGYQDVRETIDYVNHNPRPLGLYYFGRDR
                     GEEERVLTHTTSGGVTVNDVVMHVAQEDLPFGGVGPSGMGAYHGHDGFKTFSHAKAIF
                     TQSRIDVAELAGLKPPYGEKMLKAIKAQMK"
BASE COUNT          272 a          519 c          490 g          279 t
ORIGIN      
        1 gagcgaaccc tccaccgggt agcgaccctg cttcagccgc gagaacacgg cgttctggac
       61 gccgtctttc ttccactcca gcatgtcgcg gggagctacc tggggcacct cgccaagggt
      121 gagcggattg ggatcgacgt cgggtgatcg gttgatgccg agcgccgaat agcggtgctc
      181 gagctgactg agggagagct ctcggggcat ctccttcttt gcctcgagct gggcctcctg
      241 atgctccgcc ttcctccgcc gcttctgatc gagcggcgca acatcgctca tggcggaatg
      301 gaagtcttcg tcgtcactca cgtcaggccc tgagcggaat accgggcagg gaggccgagc
      361 gggtctccgg aatgggccgg ttgcctgccg cgtgataggt gaacaccagg gagagcttgg
      421 cctgatcggc gagattcata cccgcggcgt ggaacagcct gcagtggaag aagagcacgt
      481 cccccggctg cagctccacg ggcaccgccg aggcgatcat tgcctggttc tcctcgagat
      541 cggtccgcag aaacagcgcg ccgtcgagac ggccccgatc caacgacagc cggtggctgc
      601 ccggaatcac cgtcaggcct ccattcctcg cactttccgc cccgagagcc agccagaccg
      661 agatcagttc cggtcgatcg aacgaccagt agcgaatgtc ctgatgccac aacgtggcgc
      721 tggaataccc gggatgcttg gtcatcacgc agttgtggtg gcactgggac acgcgcacgt
      781 catccgggcc gaagggacgc ccgtgcactt gtgcaaacag cgcccgcagc cgatcgccga
      841 cagccagccc ggtagcccag tcgcgatagg cctgatcccg tgtgtaggca ttcagcagac
      901 gacgcacggt gttgccaccc tccgccatac ggtttgccgg cgcaccggga taacccaggt
      961 ccgcttcata ctccaccggt ccgagcaccg gactcaggtg ctgccggctg acctcgagca
     1021 ggcgcgagcg cagggtctcc gggcacagcc cccgaacgac caggtagccg tcgcgcgcaa
     1081 aggcgagcgg ctcgaggggc gctagcgcgc tcatcgcatg cactcggggc attgcgctgt
     1141 ccgcatctcc agcacgatca tcacttcatc tgggccttga tcgccttcag catcttctcg
     1201 ccgtaaggcg gcttgagtcc ggcgagctct gccacgtcga tgcggctctg ggtgaagatc
     1261 gccttggcgt gactgaacgt cttgaagcca tcgtgaccgt ggtatgcacc catgccggat
     1321 ggtcccaccc cgccgaaggg caggtcttcc tgcgccacgt gcatcaccac gtcgttcacc
     1381 gtcacgccac cggaggtggt gtgggtgagc acccgctcct cttcaccccg atcccggccg
     1441 aagtagtaca ggccgagcgg ccgcggattg tgattgacgt agtcgatggt ttcgcgaacg
     1501 tcctggtacc ccttcaccgg cagcagcggt ccgaagatct cgtcctgcat gatggtcatg
//
LOCUS       DBNHOX010000516         3977 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_40719, whole genome shotgun sequence.
ACCESSION   DBNHOX010000516 DBNHOX010000000
VERSION     DBNHOX010000516.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_40719"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="AC16UZ_11275"
     CDS             complement(<1..423)
                     /locus_tag="AC16UZ_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247309.1"
                     /translation="MDRRSFLTIAGIGAAYLLRPSLARACPTQGGSSSANWADRLGPG
                     SAMGRLGELLAPDHNGIRLPAGFSSRIVAESGNRPVSSSSYAWHAAPDGGATFAHPEG
                     GWVYVSNCEMPLGFGGASALRFDDSGTLVDAYPILGRTS"
     gene            complement(443..1975)
                     /locus_tag="AC16UZ_11280"
     CDS             complement(443..1975)
                     /locus_tag="AC16UZ_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HIR6247310.1"
                     /translation="MEILLPGRGPREPISDGGDWTFELIGRYDREIGAIAEQFGLDTY
                     PNQIEIIRSDQMMDAYASIGMPIGYHHWSFGKHFVDVERSYQRGFMGLAYEIVINSNP
                     CISYLMEENSMTMQALVIAHACYGHNSFFKGNYLFQTWTDASAIVDYLLFARNYIASC
                     EERYGVQEVEMILDSCHALMNYGVDRYKRPYPISAEEEKRRQAERAEYVQRTLNDLWR
                     TLPQARQLDSSDEEVFPREPQENLLYFIEKHAPLLEPWQREIVRIVRKVAQYFYPQRQ
                     TQVMNEGWASFWHYTLLNELYDRGLVTDGFMMEFLASHTAVLFQPSFDHPAYRGINPY
                     ALGFAIYSDLRRVCEHPTDEDREWFPDIAGGNWLEVLPAAMRDFKDESFVLQFLSPRV
                     MREMRLFGVVDDDAEDEVLISAIHDRGGYREVRSLLAGQYNIGDREPNIQVVRVDVRG
                     DRSLTLRHAQHARQPMGQSTDEVMRHLHRLWGFPVRLESVWHDEVVATTACPAEEAQS
                     AA"
     gene            complement(1975..3243)
                     /locus_tag="AC16UZ_11285"
     CDS             complement(1975..3243)
                     /locus_tag="AC16UZ_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009685232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HIR6247311.1"
                     /translation="MSYIIDRRENAKNKSAVNRQRFLERYKQRIKKAVSDAVNERSIT
                     DMERGEEVTIPAQDISEPVFQHGSGGRRSIVHPGNKEFIAGDRIARPEGGAGGGSGDG
                     EASNSGEGSDDFVFQLTQEEFLEYLFDDLALPNLTKRHLKGNSSFKYEHAGFTSDGVP
                     AKLSVLRSLRQAKARRIALAGEPKRLLREAREAFDQAELDGDELQRRRMRDEIERLEK
                     RLGRIPFLDTNDLRYNLHLRKPVPTSQAVMFCLMDVSGSMDQHIKDMAKRFYLLLYMF
                     LKRHYERTEIVFIRHHTVAKEVDEQEFFYSRETGGTVVSSALKLMLEVLEDRYSPNEW
                     NIYGAQASDGDNWNDDSPLCRQLLEKKILPLVQYFSYVEITKRSHQALWREYEHLAER
                     FPESFAQRQITDVSDIFPVFHDLFQKKDAA"
     gene            complement(3319..>3977)
                     /locus_tag="AC16UZ_11290"
     CDS             complement(3319..>3977)
                     /locus_tag="AC16UZ_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HIR6247312.1"
                     /translation="ILSKVFNFDAIEVAANPVHLMYVLEQQIEQEQYPQEVRDRYLRF
                     IKEYLAPKYVDFIGKEIQTAYLESYSEYGQNVFDRYVTYADFWIQDQEYRDPETGDIL
                     DRASLNEELEKIEKPAGISNPKDFRNEIVNFVLRARASNNGTNPSWLSYEKLRTVIEK
                     KMFSNTEDLLPVISFNAKSSSDEQRKHDDFVARMVERGYTDKQVRLLAEWYLRVRKSQ
                     "
BASE COUNT          717 a         1232 c         1254 g          774 t
ORIGIN      
        1 gctcgttcgg ccgaggatcg ggtaggcgtc taccagggtt cccgaatcat cgaaccgcag
       61 ggcgctggca ccgccgaacc ccagcggcat ctcgcagttg ctgacgtaga cccaacctcc
      121 ttcgggatgc gcaaacgtag cacctccatc cggggccgcg tgccaggcgt aggacgagga
      181 cgacacgggg cgattgccgg actcggcaac gatcctcgag ctgaacccgg caggcagccg
      241 gataccgttg tgatccgggg caagcagctc gcccaggcgg cccatcgcgg acccagggcc
      301 cagtctgtcc gcccaattgg cagacgacga cccaccttgg gtagggcagg cgcgagcgag
      361 cgagggcctc agcagatacg cggcgccgat gccggcaatg gtcaggaaag atcgtctgtc
      421 catctcggca ttgtaacagg ggctacgccg ccgactgagc ctcctccgcg gggcaggcgg
      481 tggtggcaac cacctcgtcg tgccagacgg actccaggcg aacgggaaat ccccagagcc
      541 gatgcaggtg ccgcatgacc tcgtcggtgc tttgacccat gggctggcgc gcatgctgcg
      601 cgtgccgcag cgtgaggctc cggtcacccc tgacgtcgac ccgcaccacc tgaatgttcg
      661 gctcccggtc gccaatgttg tactgcccgg ccagaaggct gcgcacctcg cggtagccgc
      721 cccgatcgtg aatggccgag atgagcacct cgtcttcggc gtcgtcgtcg acgacgccga
      781 acagacgcat ctcgcgcatg accctggggc ttaagaactg cagcacgaag ctctcgtcct
      841 tgaagtcccg catggcggcc ggcagcacct cgagccagtt gcccccggcg atgtcgggaa
      901 accactcgcg gtcctcgtcc gtcggatgct cgcacacgcg ccgcagatcc gagtagatgg
      961 caaagcccag cgcgtaggga ttgatgcccc ggtacgcggg gtgatcaaaa ctcggctgga
     1021 acagaaccgc tgtgtgcgag gcgagaaact ccatcatgaa gccgtcggtg accaggccgc
     1081 gatcgtagag ctcgttcaaa agggtgtaat gccagaacga cgcccacccc tcgttcatca
     1141 cctgggtctg ccgctgaggg tagaagtact gcgccacctt ccggacgatg cgcacgatct
     1201 cgcgctgcca gggctccaag agcggcgcgt gcttctcgat gaagtagagg agattttcct
     1261 gaggctcccg cgggaagact tcctcgtcgc tcgagtcgag ctgccgggcc tggggaagcg
     1321 tccgccacag atcgttgagc gtgcgctgga cgtattcggc gcgttcggcc tggcgccgct
     1381 tctcttcctc tgccgagatc gggtacggcc gcttgtagcg gtcgaccccg tagttcatca
     1441 gggcgtgaca ggagtcgagg atcatctcca cttcctgcac gccgtagcgc tcctcgcagc
     1501 tggcaatgta gttacgggcg aagagcaggt agtcgacgat ggcgctggcg tccgtccagg
     1561 tctggaacag gtagttcccc ttgaagaaag agttgtggcc atagcaggca tgggcgatga
     1621 ccagggcctg catggtcatg ctgttctctt ccatgaggta ggaaatgcag ggattcgagt
     1681 tgatcacgat ctcgtaggcc aggcccatga atccgcgctg gtagctgcgc tcgacgtcga
     1741 cgaagtgctt gccgaaggac cagtggtgat agccgatggg catgccgatg gaggcatagg
     1801 catccatcat ctggtccgaa cggatgatct cgatctgatt ggggtacgtg tcgagaccga
     1861 actgctcggc aatcgcgccg atctcgcggt cgtaccgacc gatgagctcg aacgtccagt
     1921 ccccgccgtc ggagatgggc tcgcgcggcc ccctgcccgg caagaggatt tccatcacgc
     1981 ggcgtccttc ttctggaaga ggtcgtgaaa cacgggaaag atgtccgaca cgtcggtgat
     2041 ctggcgctgg gcaaacgatt ccggaaaccg ctcggccagg tgctcgtact cccgccacag
     2101 cgcctgatgg ctgcgcttgg ttatctccac gtaggagaag tactgcacca gaggcaggat
     2161 cttcttctcc agaagctgcc ggcagagggg cgagtcgtcg ttccagttgt ccccgtccga
     2221 ggcctgggcc ccgtaaatgt tccactcgtt gggcgagtac cgatcttcca gcacctcgag
     2281 catcagcttg agcgcgctgg agaccacggt cccgcccgtt tccctggaat agaagaactc
     2341 ctgctcgtcc acctccttgg caacggtgtg atgccggatg aagacgatct ccgtgcgttc
     2401 gtaatggcgc ttcaagaaca tgtagagcaa caggtagaag cgcttcgcca tgtccttgat
     2461 gtgctggtcc atggagccgg acacgtccat gaggcagaac atcacggcct gggaggtggg
     2521 caccggcttg cgcaagtgca ggttgtagcg cagatcgttg gtatccagaa agggtatgcg
     2581 gccaagccgt ttttccaagc gctcgatctc gtcgcgcatg cggcgacgct ggagctcatc
     2641 gccgtcgagc tccgcctgat cgaacgcctc gcgcgcctcg cgaagcagcc gcttgggctc
     2701 gccggccagc gcgatgcgcc gcgccttcgc ctgccgcagg gaacgcagca ccgagagctt
     2761 ggcgggcacc ccgtcgctgg tgaacccggc atgttcgtac ttgaagctcg agttgccttt
     2821 cagatgccgc ttggtgaggt tgggcagggc caggtcgtcg aagaggtact cgaggaactc
     2881 ctcctgggtg agctgaaaga cgaagtcgtc ggagccctca ccagagtttg aggcctcgcc
     2941 atccccggaa ccgccgccgg cgccgccttc cggacgcgca atgcggtctc cggcaatgaa
     3001 ctccttgttg ccgggatgca cgatgctgcg gcgcccgccg ctgccgtgct gaaagaccgg
     3061 ttcggagatg tcctgggcgg ggatggtgac ttcctcgccc cgctccatgt cggtgatgct
     3121 gcgctcgttc accgcatccg ataccgcctt cttgatgcgc tgcttgtaac gctccagaaa
     3181 acgctgccga ttgacggcgc tcttgttctt ggcgttttcc cggcggtcga tgatgtagct
     3241 catgccatgc ccccgctgcg cggtgcctct caccgtcccg ggggcatcag cctccgggac
     3301 ggtgagaggg ttccgttgct actgtgactt gcgaactctc aggtaccact ccgcgagcag
     3361 tcgcacctgc ttgtcggtat aaccacgctc caccatccgg gcgacgaagt cgtcgtgctt
     3421 gcgctgctcg tcgctggagg acttcgcgtt gaaagagatc accggcagaa gatcctcggt
     3481 gttggagaac atcttcttct ctatgaccgt gcgcagcttc tcgtagctca accacgacgg
     3541 gttggtgccg ttgttgcttg cacgcgccct cagcacgaaa ttgacgatct cgttgcggaa
     3601 gtctttcgga ttgctgatgc cggcgggctt ctcgattttc tccagctcct cgttcaggga
     3661 agcgcgatcg agaatgtcgc cggtttccgg gtcgcgatac tcctgatcct gaatccagaa
     3721 atcggcgtat gtgacgtagc ggtcgaagac gttctgaccg tactcggagt aggactcgag
     3781 gtacgccgtc tggatttcct tgccgatgaa gtccacgtac ttgggagcca ggtactcctt
     3841 gatgaagcgc agataccggt cccggacctc ctgaggatac tgctcctgct cgatctgctg
     3901 ctcgagcacg tacatcaggt gaacggggtt ggcggccacc tcgatggcgt cgaaattgaa
     3961 caccttcgaa aggatct
//
LOCUS       DBNHOX010000517         2075 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_427033, whole genome shotgun sequence.
ACCESSION   DBNHOX010000517 DBNHOX010000000
VERSION     DBNHOX010000517.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_427033"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="AC16UZ_11295"
     CDS             complement(<1..837)
                     /locus_tag="AC16UZ_11295"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HIR6247313.1"
                     /translation="MADGAYLVADIGGTNARLALTDGTRLTSPVLISQTASLRNVAGL
                     AEAARSAFDGQALDGCCIAMAGPVHANQGSLTNVSLAVSGDALGAELGCPARVVNDFY
                     AVARGIPFLRHLRQIGPGEPDEGVRALLGPGSGLGMAVLAPTREGPPLVLPSEGGHAD
                     LAPGSPLEQEVLQVLAARLPHVSWESVLSGPGLVNLYCALCAIWGVPEEALAPEEISR
                     QGLVVEDPICHQVLEMFFGFLGAAAGNLALTVCAEGGVFIGGGIVPKLVEFAESSPLR
                     RRF"
     gene            complement(837..1910)
                     /locus_tag="AC16UZ_11300"
     CDS             complement(837..1910)
                     /locus_tag="AC16UZ_11300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6247314.1"
                     /translation="MEDRRYLVVGARGLVGRAVLSALEAEDRAEVIGLSRRQPDFPTR
                     ARWIPCDLTDRDACHAALGAAQTQGVTHIVYAALQEMPDLISGWRDPVQIETNARMLA
                     NLLEFVQPREHFTLLQGTKAYGAHLAPMALPGKESAPRHPGENFYWEQEDLMRSRQAG
                     SSWAFSIVRPQIVCGVALGSPMNMVAAIGVYAAVMRQLGKPLCFPGCGGFVTEATDAD
                     LLARAIIWAGREPACANEILNVTNGDVLDWVSLWPAFGEIFQMPTGEADPMRLAISMP
                     ELSGVWDEVVGRHGLKSMAMAELVGDSWQFADAVFGYGGGARSTLLSTIKIRQLGFHE
                     CIDTEQMFRNQLAALQDLKVLPR"
BASE COUNT          360 a          726 c          672 g          317 t
ORIGIN      
        1 gaagcgcctg cgcagggggc tggactccgc gaactcgacg agcttgggca cgatgccgcc
       61 gccgatgaat acgccgcctt ccgcacagac ggtgagcgcg aggttccccg ccgcggcgcc
      121 gagaaagccg aagaacattt ccagcacctg atggcagatc gggtcctcca ccaccagccc
      181 ctgcctgctg atctcctcgg gagccagcgc ttcctccggc actccccaga tggcgcatag
      241 cgcgcagtag aggttcacca ggccgggtcc tgacagcacg ctctcccagc tgacgtgggg
      301 gagccttgcg gcaagcacct gcagcacctc ctgctccagg ggagagccgg gcgcgaggtc
      361 ggcgtgcccg ccctcggacg gaagcaccag gggcggacct tcccgggtcg gcgccagcac
      421 ggccatcccc aacccgctgc cgggtccaag cagcgccctg acgccctcgt cgggctcgcc
      481 cggtccgatc tgacgcaggt ggcgcaggaa cgggatgccc cgagccacgg cgtagaaatc
      541 gttgaccaca cgggcgggac agccgagctc ggcacccagc gcgtcccccg aaacggccag
      601 cgacacgttc gtcaggctgc cctgattggc gtgaacgggg cccgccatgg cgatgcagca
      661 cccgtccaac gcctggccgt cgaaagccga acgcgcggct tcggcgagac cggccacgtt
      721 cctgagcgag gccgtctgcg aaatcagcac gggggacgtg agccgcgttc cgtcggttaa
      781 cgccaggcgg gcattggtgc caccgatgtc cgcgaccaga tacgctccgt ccgccatcag
      841 cgaggcagca ccttgagatc ctgcagggcg gcaagctggt tgcggaacat ctgctcggtg
      901 tctatgcatt cgtggaagcc gagctggcga atcttgattg tgctcagcag cgtgctccgc
      961 gccccgccgc catagccgaa taccgcgtcg gcgaactgcc atgaatcgcc caccagctcg
     1021 gccatggcca tggacttaag cccgtgccgt ccgaccacct cgtcccagac accggagagt
     1081 tccggcatgc tgatcgcgag ccgcatgggg tccgcttcgc cggtgggcat ttgaaaaatc
     1141 tcaccgaacg cgggccacag ggagacccaa tcgagcacgt cgccgttggt gacgttgagg
     1201 atctcgttgg cgcaggcggg ttcgcgcccg gcccagatga tcgcgcgggc cagcaggtcg
     1261 gcgtccgtgg cctcggtaac gaaaccgccg caacccggaa agcaaagcgg cttgccgagc
     1321 tggcgcatca cggcggcgta aacaccgatg gccgccacca tgttcatggg gctgccgagc
     1381 gctacgccgc agacgatctg tgggcggacg atgctgaagg cccaggacga tccggcctgg
     1441 cggctgcgca tcaggtcttc ctgctcccag tagaagttct cgcccgggtg ccggggcgcc
     1501 gactccttcc ccggcaacgc catgggcgcg agatgagcgc cgtaggcttt ggtcccctgc
     1561 agcagcgtga agtgctcccg tggctgcacg aactccagca ggttggcgag catccgcgcg
     1621 ttggtttcga tctggacggg gtcccgccag cccgatatca gatccggcat ttcctgaaga
     1681 gcggcgtaga cgatgtgggt aacgccttgc gtctgcgcgg cgcccaacgc cgcgtggcac
     1741 gcatcgcggt ccgtcagatc gcagggaatc cagcgcgccc gggtgggaaa gtccggctgc
     1801 cgacgggaaa gcccgatgac ctctgctcga tcttcagcct ccagagccga cagcacggcg
     1861 cggccgacga gcccccgggc ccccacgacc agatatctgc gatcctccat gccgccattg
     1921 tccgcatgcc acccatccgg ttcaattgcc aggccaccaa ctcggcccgc tctaatccgg
     1981 ttcccccgaa gccaggattg attcatgacg gtcagcgccg gactcggcat cgcgaatttt
     2041 ccgttccagg acgggccaga cttctggcgc tgggt
//
LOCUS       DBNHOX010000518         4414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1455067, whole genome shotgun sequence.
ACCESSION   DBNHOX010000518 DBNHOX010000000
VERSION     DBNHOX010000518.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1455067"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="AC16UZ_11305"
     CDS             complement(<1..464)
                     /locus_tag="AC16UZ_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013328.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HIR6247315.1"
                     /translation="MPELPEVETTRRGVAPLVDGRYLLDWNLRNPNLRWPVQLPDELR
                     GSRIARVRRRAKYLIFELEPSALGAAGSLIIHLGMSGNLRVLPQGSPYLPHDHVELAL
                     ASEGEVAATRVLRLNDPRRFGSLHWQPGEAEAHWLLAGLGPEPLSEAFSGEYL"
     gene            complement(493..648)
                     /gene="rpmG"
                     /locus_tag="AC16UZ_11310"
     CDS             complement(493..648)
                     /gene="rpmG"
                     /locus_tag="AC16UZ_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR6247316.1"
                     /translation="MRDKIKLVSSAGTGHYYTTDKNKRTTPDKLEMKKYDPVVRKHVV
                     YTEAKIK"
     gene            complement(674..910)
                     /gene="rpmB"
                     /locus_tag="AC16UZ_11315"
     CDS             complement(674..910)
                     /gene="rpmB"
                     /locus_tag="AC16UZ_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR6247317.1"
                     /translation="MSRVCQVTGKRPITGNNVSHAHNKTRRRFSPNLHYHRFWVDSEK
                     RFVRLKVSSQGMRIIDKLGIDQVLADMRKRGEKV"
     gene            complement(984..1658)
                     /gene="radC"
                     /locus_tag="AC16UZ_11320"
     CDS             complement(984..1658)
                     /gene="radC"
                     /locus_tag="AC16UZ_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR6247318.1"
                     /translation="MATVKWPDSDQPRNRLLQAGPDRLTDAELLAVCLRTGHGPQDPV
                     TYARSLLRRFGHIGKLLDASPDRLLGMPGMGPAKAAALKASLGVAERYLRAGLGGGRL
                     MGDSRAVTDFLKHQLGSLQREVFACLFLDARHRFIAFEKMFLGSLDRASVHPREVLKR
                     TLELNAAAVIFAHNHPSGVAEPSASDVQITEELKALLERLEVCVLDHVVIGRGCQVSF
                     AERGLL"
     gene            1739..3016
                     /gene="coaBC"
                     /locus_tag="AC16UZ_11325"
     CDS             1739..3016
                     /gene="coaBC"
                     /locus_tag="AC16UZ_11325"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017526818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR6247319.1"
                     /translation="MTQPDNQAPANLGGRKILLGVSGGIAAYKAPILVRLLRQAGADV
                     QVVLTRSATRFVTATTLQAVSGRPIRDDLWDAAAEAAMGHIELARWADAVLIAPATAE
                     MLSRLATGRADDLLTTLVLATRAPIFVAPAMNNAMWDHPATQRNASTLTDRGVNVLGP
                     DSGEQACGEFGPGRMREPADLVADLCASFEPKDHLLAGKNVLVTAGPTREAIDPVRYV
                     TNHSSGKQGYAMAAAARAAGAQVTLISGPVHLPTPIGVRRIDVTSAAEMHQAVLAHAS
                     TCDVFIGVAAVADYRPDRAHDQKIKKTDTGSGESGLSLRLVENPDIIAGVSSLERRPF
                     VVGFAAETHDALSHARDKRKRKGMDLIVVNDVSDSSIGFNSDQNAATVIWEDGELSIP
                     RQGKDLVARAVIDLIARQIRDPLAGANPERIAK"
     gene            3088..>4414
                     /locus_tag="AC16UZ_11330"
     CDS             3088..>4414
                     /locus_tag="AC16UZ_11330"
                     /inference="COORDINATES: protein motif:HMM:NF014877.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247320.1"
                     /translation="MESDKDDQPAAKKTTGKPAKKRSKPAPTGAFGSTVWAPLLGTLG
                     VVCAFALLWLQLIESGNANHRELQTAVHRDFLVAHFNDRIANLRNQVLSIARTRTVTE
                     AATSEDPATIADAGSALSDLSAFALRVDIFRPGQAVLDKEAEVPISYAALDLIRRAEA
                     AAMVGPEGASINQRGYVYMAAQIGEPGSRAGVLFAVVSPDFFLSGLASLDERIGRVRI
                     EQLSESVDPIEIFGWGGQSDPAAAEIVTLENQKWNLVFEPGSAILQPVTKMVDLALPF
                     GVALLLVIAGGYLSTTRVTKRLRQAAAAMPVQAQPPEEAPAEETAVEEEAEPVGEEAE
                     PGAGAASTLPSRGFTDDAKDNFGIEVSEEVSLGEMGIDLDPQIFRAYDIRGIVTDNLT
                     EDVVYWIGRAFAAEALAQEQTRAAVGRDGRHSSEALRTALIKGLTDGGLD"
BASE COUNT          804 a         1478 c         1410 g          722 t
ORIGIN      
        1 agatactctc ccgagaaggc ctcagagagc ggctccgggc cgagccccgc caggagccag
       61 tgggcttcgg cctcacccgg ctgccagtgc aggctgccga accgacgcgg gtcattcagc
      121 cgaagcacgc gcgtcgcggc cacctcgccc tccgatgcga gggccaactc cacgtgatcg
      181 tggggcaggt agggcgagcc ctggggcaga acccgcaggt ttccagacat gcccaggtgg
      241 atgatgagac tgcccgcagc gcccagggcc gatggctcga gctcgaatat gaggtacttg
      301 gcccggcgcc tgactctggc tatccgggaa ccgcgcagct catcgggtag ctgcaccggc
      361 cagcgcagat tggggttgcg cagattccag tcgagcaggt agcgcccgtc gaccaggggt
      421 gctacgccgc ggcgggtggt ctcgacttca ggtagttcgg gcaccgatca gcagggggcg
      481 ctgatccgcc ggttacttga tcttcgcttc ggtatagacg acgtgcttgc ggacgacggg
      541 atcgtacttc ttcatctcca gcttgtccgg cgtcgtacgc ttgttcttat cggtggtgta
      601 gtagtgtccg gtgcccgcac tggaaaccag ctttatcttg tcgcgcatct gtctttacct
      661 gccgttagcc cactcagacc ttctcgccgc gcttgcgcat gtcggcaagg acctgatcga
      721 tgcccagctt atcgattatg cgcatgccct gggacgaaac cttcagccgc acgaagcgct
      781 tctcgctgtc aacccagaag cgatggtaat gcaggttagg cgaaaaacgc cggcgcgtct
      841 tgttgtgcgc atgactgacg ttgttgccgg tgatcggccg cttacccgtt acctgacaaa
      901 cgcgcgacat ggtcccgttt tcctggcgaa aaagaggcgc gtttatacca gagcgccctt
      961 cggccggcaa cagccggccg cgttcagagc aggcctcgct cggcgaagga gacctggcaa
     1021 ccgcgcccga tcacgacgtg atcgagcacg cacacctcca gccgctccag cagcgctttg
     1081 agctcttcgg tgatctggac gtcgctggcg ctcggctcgg cgacgccgga gggatggttg
     1141 tgagcgaaaa tcaccgccgc cgcgttgagc tccagagtcc gtttaagcac ctctcttgga
     1201 tgcacgctgg cccgatccag cgatccgaga aacatcttct cgaaggcgat gaaccgatgc
     1261 ctggcgtcca ggaacaggca ggcgaacact tcccgctgca aactgccgag ctgatgcttg
     1321 agaaagtcgg tcacggcccg agagtcaccc atcagacggc cgccgccgag gccggcacgc
     1381 aggtaccgct cggcgacccc caggctggct ttcagcgcgg cggctttcgc cggccccatc
     1441 ccaggcattc cgagcaggcg atcgggagag gcgtccagca gcttgccgat gtgcccaaaa
     1501 cgccgaagca gcgagcgcgc atacgtaacc gggtcctggg ggccgtgccc ggttcttagg
     1561 cacacggcga gcagctcggc gtcggtcaga cgatcgggtc cggcctggag aagacgattt
     1621 cggggctggt cactgtcagg ccatttgacc gtggccatgg gaaaacctcc tgcggcgaat
     1681 cggtggcggt ggtatcttta ggctcaatca gtttccagcc aggcccaaca gctaaacgat
     1741 gacgcagcca gacaaccagg cgccggcaaa tctcgggggc agaaagatcc tactcggggt
     1801 cagcggaggc atcgcggcgt acaaggcccc aattcttgtg cgccttctgc gacaggccgg
     1861 ggcagacgtc caggtggttc tgacccgcag cgcgacgcgg ttcgtcaccg cgactaccct
     1921 gcaggccgtc tccggcaggc ccatcaggga cgacctctgg gacgccgcgg ccgaagccgc
     1981 catgggccac atcgagctcg cccgctgggc cgacgccgtg ctcatcgccc cggcaactgc
     2041 cgagatgctc agccggcttg ccaccggccg cgccgacgac ctgctgacga ccctggttct
     2101 ggccactaga gcgccgatct tcgtcgctcc cgcgatgaac aacgccatgt gggatcaccc
     2161 ggccacccag cgcaatgcga gcaccctgac agaccggggc gtgaacgtgc tggggcccga
     2221 cagcggcgag caggcgtgcg gggaattcgg tccgggccgc atgcgcgagc ccgcggatct
     2281 ggttgccgac ctgtgtgcgt ctttcgagcc taaggaccat ctgctggccg gcaagaacgt
     2341 cctcgttaca gccgggccca cccgcgaggc catcgacccg gttcgctacg taaccaacca
     2401 cagctccgga aagcaggggt acgccatggc cgccgccgca cgggccgccg gtgcccaggt
     2461 gaccctgatc tccggacccg tgcacctccc cacacccatc ggcgtgcggc gcatcgacgt
     2521 cacgagcgcc gcagagatgc accaggccgt cctggcacac gccagcactt gcgacgtgtt
     2581 catcggcgtg gccgcggtag ccgattatcg tcccgaccgc gctcatgacc agaagataaa
     2641 gaaaaccgac acaggatccg gcgaatcggg cctctccttg agactggtcg agaacccgga
     2701 catcatcgcc ggcgtatcca gcctggagcg acgtcctttc gtcgtcgggt tcgccgccga
     2761 gacccacgat gcgttgagcc acgcgcgcga caagcgcaag cgcaagggca tggacctcat
     2821 cgtcgtcaac gacgtgtcgg attcgagcat tggtttcaac agcgaccaga atgctgccac
     2881 ggtcatctgg gaagacggag agctgtcgat tccgcggcag ggcaaggacc tcgttgcccg
     2941 cgcggtgatc gatctgattg cccggcagat acgcgacccg ttagcgggcg caaaccccga
     3001 acgcatcgca aaatagcgcg cggcgctgga ttgccctggc atatccgcat taccattgcc
     3061 ccgccccgga cgcctggatc ctgacccttg gaaagcgaca aagacgacca gcccgccgcc
     3121 aagaagacca ccggaaagcc cgctaagaag agatcaaagc ccgctcccac cggcgcgttc
     3181 ggctcgacgg tctgggcacc gcttctcggc actctcggcg tggtctgcgc cttcgcgctg
     3241 ctttggctgc agctgatcga atccggcaac gccaatcatc gagagttgca gacggccgtg
     3301 caccgcgact tcctcgtcgc ccacttcaac gaccgcatcg ccaatctgcg caatcaggtg
     3361 ctctccatcg cgagaacgcg caccgtcacc gaggcagcca ccagcgaaga cccggccacc
     3421 atcgccgatg caggcagcgc gctgagcgac ctgtccgcct ttgccctgcg cgtggacatc
     3481 ttccggccag gccaggccgt cctggacaaa gaagccgagg tgccaatttc ctacgccgcc
     3541 ctcgatctga tcagaagagc cgaggccgcg gcgatggtgg ggcccgaggg cgcatcgatc
     3601 aatcagcgcg gttatgtcta catggctgcg cagattggag agccgggctc ccgcgccggg
     3661 gtgttgttcg ccgtggtctc tccggacttt ttcctgagcg ggctggcctc gctggatgag
     3721 cgcatcgggc gcgtgcgcat cgagcagctc tccgagagcg tcgaccccat cgagattttc
     3781 ggctggggcg gccaatccga ccccgccgcc gcggagatcg tcacgctgga gaaccagaaa
     3841 tggaatctcg tctttgagcc cggttcggcg attctgcagc cggtgaccaa gatggtcgac
     3901 ctggcactgc cattcggggt cgcccttctg ctggtaatcg ccggcggata cctctccacc
     3961 acgcgcgtta ccaagcgtct gcgccaggcc gccgccgcca tgccggtgca ggcccaaccg
     4021 cccgaggaag cgccagcgga ggagacggcc gtcgaggagg aagccgagcc cgtcggcgag
     4081 gaggccgagc cgggagcggg agcggcaagc acgctgccca gccgcggctt taccgacgac
     4141 gccaaggaca acttcggcat cgaggtcagc gaggaagtgt ctctgggcga gatggggatc
     4201 gatctggacc cgcagatatt ccgggcctac gacattcgcg gcatcgtcac cgataatctg
     4261 accgaggacg tcgtgtactg gatcgggcgg gcgtttgccg ccgaggcgct ggcccaggag
     4321 cagacccggg cggccgtagg ccgcgatggc cgacactcga gcgaggcgct gcgcaccgcg
     4381 ctcatcaagg gcctgaccga tggcggtctc gacg
//
LOCUS       DBNHOX010000519         2062 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_684309, whole genome shotgun sequence.
ACCESSION   DBNHOX010000519 DBNHOX010000000
VERSION     DBNHOX010000519.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_684309"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="AC16UZ_11335"
     CDS             <1..571
                     /locus_tag="AC16UZ_11335"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6247321.1"
                     /translation="LDEVASGADNVTVQQMDAGDPASIEAAAAAIGDAPIDLLINNAG
                     AVGGQHQSLDDVDIAEWHRTLDINTIGPLLISRAFKSNLAASGDGKLMIVTSQLAAST
                     WPMGGMYIYSTTKAGVSKVGQILALDWKEEPITVGLMHPGWVQTDMGGAHAEITAEES
                     ASGIRSVIAGMTKADSGSFYKWNGEIHPW"
     gene            603..>2062
                     /locus_tag="AC16UZ_11340"
     CDS             603..>2062
                     /locus_tag="AC16UZ_11340"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6247322.1"
                     /translation="MSSHRIEVTTLGDLLLRAADRHGDRDAIIFPEARLTYDQLREGA
                     YARARSLLALGVDRGDHVGILMPNCLDYVECLFAVQLLGAMAVPINARYRARELAYVV
                     ENADLSAILISDVISEYADFAELLQEAFPELSANADPRALELPGAPKLKTVVMLGSSA
                     PAGILPRAELDALASGYQVEDVEARRVCARVRDQAIMMYTSGTTSDPKGCPLSHEVLV
                     RNGFNMNRERYFLDETDCFWAPLPMFHMASILPMICCMDAGAALQSMTHVEAGGALAM
                     MERDATTVAFPSFPTVTNELINHPEFTTRDLSRIRRINNVAPPDVLRKFQDAFPQAVQ
                     TGAYGLTEVGGVIAFNHPDESLEQRLTTCGRPFPGVEVKVVDPDTGAELGPGERGEIL
                     VRGYAVFDGYYKAPEKNAEAFADGWFRTGDLCALDADGSIEFHGRIKDMLKVGGENVA
                     ALEIESYLATHPAVKLAQVIGVPDERLLEVPAAFIEL"
BASE COUNT          362 a          695 c          665 g          340 t
ORIGIN      
        1 gcttgacgag gttgccagcg gcgcggacaa cgtcaccgtt cagcagatgg acgccggcga
       61 cccggcctcc atcgaagcgg cggctgctgc catcggcgat gcccccatcg acctcctgat
      121 caacaacgcc ggcgccgtgg gcggacagca ccagtccctc gacgacgtcg acatcgcgga
      181 gtggcatcga accctggaca tcaacaccat cgggccgctg ctcatctccc gggcgttcaa
      241 gagcaacctg gcagcttccg gcgacggcaa gctgatgatc gtcacgagcc agctcgcggc
      301 ctccacgtgg cccatgggcg ggatgtacat ctactccacg accaaggccg gggtgagcaa
      361 ggttggtcag attctggcgc tggactggaa ggaagaaccc atcaccgtgg gtctcatgca
      421 tccaggctgg gtgcagaccg acatgggcgg tgcccacgcc gagatcaccg ccgaggagag
      481 cgcatcgggc atccgcagcg tgatcgccgg catgaccaag gcagactcgg gcagcttcta
      541 caagtggaac ggcgagatcc atccctggta gcaggtttcc tggcacccgt gaaggtgtcg
      601 ggttgagctc acaccgcatc gaggtcacca cccttggcga tctgctgctg cgcgcggcgg
      661 atcgccacgg cgaccgtgac gccatcatct ttcccgaggc acggctcacc tacgaccagc
      721 tgcgagaggg cgcctacgcc cgcgcaagga gcctgttggc tctgggcgtc gaccggggcg
      781 accacgtcgg catcctgatg cccaactgcc tggactacgt ggagtgcctt ttcgccgtcc
      841 agctgctcgg cgccatggcg gtaccgatca acgctcgcta cagggcccgg gagctggctt
      901 acgtggtgga gaacgccgat ctctcggcca tcctgatcag cgacgtcatc tccgagtacg
      961 ccgacttcgc cgagctgctg caggaagcgt tccccgagct gtccgccaat gcggatcccc
     1021 gggctctcga gctgcccggc gcgccgaagc tcaagacggt cgtgatgctc ggaagctcgg
     1081 cgcctgccgg catcttgccc cgggccgagc tcgacgcgtt ggcgtccggg taccaagtcg
     1141 aggacgtgga ggcccgccgg gtctgtgccc gggtgcggga ccaggccatc atgatgtaca
     1201 cctccggcac cacctcggat ccgaagggtt gcccgctcag ccacgaggtg ctggtgcgaa
     1261 acggcttcaa catgaaccgc gagcgctact tcctcgacga gaccgattgc ttctgggctc
     1321 ccctgccgat gttccacatg gcctccatcc tgcccatgat ctgctgcatg gatgccggtg
     1381 ccgccctgca gtccatgacc cacgtcgagg ccggcggggc cctggcgatg atggagcgcg
     1441 acgcaaccac cgttgccttc ccgtcctttc cgacggtcac caacgagctg atcaaccatc
     1501 ccgagttcac cacccgggac ttgagccgta tccgccggat caacaacgtc gcgccgccgg
     1561 acgtgctgcg gaaatttcag gacgcgtttc ctcaagccgt gcagacgggc gcctacgggc
     1621 tcaccgaggt gggcggcgtg atcgccttca accacccgga cgagagcctg gagcagcgtc
     1681 tcaccacctg cggccggcca tttcccggcg tcgaggtcaa agtggtggat ccggataccg
     1741 gcgccgagct gggaccggga gaacgcggcg agatcctcgt acgcggctac gccgttttcg
     1801 acggctacta caaggcgccg gagaagaacg cggaggcttt tgccgacggc tggttccgga
     1861 ccggcgatct gtgcgccctt gacgccgacg gctccatcga gtttcacgga cgcatcaagg
     1921 acatgctcaa ggtgggggga gagaacgtgg cggcgctgga gatcgagtct tacctggcaa
     1981 cgcatccggc agtgaagctc gcccaggtga tcggcgtgcc cgacgagcgg ctgctggaag
     2041 tgcccgccgc tttcatcgag ct
//
LOCUS       DBNHOX010000520         2369 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1327482, whole genome shotgun sequence.
ACCESSION   DBNHOX010000520 DBNHOX010000000
VERSION     DBNHOX010000520.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1327482"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="AC16UZ_11345"
     CDS             <1..426
                     /locus_tag="AC16UZ_11345"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6247323.1"
                     /translation="FDGAINYKTQDVAAELDRLCPNGIDVYFDNVGGEILDICLDRIA
                     LNARVVLCGGISRYNVEGELPGPKNYFNLIFRRARMQGFLAPDYAPRFDEAYAELQGW
                     LAEGKIRQRETVIEGFENLPKALVLLFEGANTGKMMVKV"
     gene            complement(484..1365)
                     /locus_tag="AC16UZ_11350"
     CDS             complement(484..1365)
                     /locus_tag="AC16UZ_11350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6247324.1"
                     /translation="MKFGLSMFTTDYSASPTEVAKAAEANGFESFWVSEHSNIPVDTD
                     FPMSDTVPREYSSMLDPFVALAAAATVTERINLGTAICLVTQRDPINCAKLVASIDQL
                     SGGRMLFGIGAGWNEPEMRNHGTDPNTRFRLMRERVEAMKALWTNEVAEYHGRLVNIE
                     PTWQWPKPVQQPHPPVIVAGAGPNVLKRVIAYGEGWLPVVTPVAPEQMKGRVTPMDEF
                     VEMAGELERMADAAGRPTPAITVSGMNLDAATFDAFAELGVERMILRLTTAPLDDVLA
                     QIEQHVETVRSAGGSLH"
     gene            complement(1405..>2369)
                     /locus_tag="AC16UZ_11355"
     CDS             complement(1405..>2369)
                     /locus_tag="AC16UZ_11355"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6247325.1"
                     /translation="IVSKSKAGRLDEIRPCIGCNDCLHAGVVEGLPFGCTVNPRTGYE
                     HKPPPAPASPPKNLLVVGGGPAGLELAAAAAERGHTVTLWERDDALGGQMRLAAQVPE
                     NRAFGDFIEFQERRLARLGVTVETGREATAESIAEAGFDVVAIATGATPRGHEASGAH
                     LPFVLEGRDVMSGEAKPGKRVAVIAMEDHLQPLTVASFLADRGHEVQLIYQTPSIAPL
                     IGKYSIGAPLAKITASGARVRVMERVTSIEQGRIETANVYSGAGSEITDFDSVVLACG
                     GVADSGLYHSLKGKVDELHVLGDAYAPRRIWFATRQAYALAATL"
BASE COUNT          359 a          796 c          808 g          406 t
ORIGIN      
        1 ttcgacggcg ccatcaacta caaaactcag gacgtggccg cagagctcga ccgtctgtgc
       61 ccgaacggca tcgacgtcta cttcgacaac gtgggcggcg agatcctcga catctgcctg
      121 gaccggatcg cgctcaacgc ccgggtcgtg ctctgcggcg gtatctcccg ctacaacgtg
      181 gaaggagagc tgccggggcc gaagaactac ttcaacctga tcttccggcg ggcgcgcatg
      241 cagggctttc tggccccgga ttacgccccg cgcttcgacg aggcctacgc cgagctgcag
      301 ggctggcttg ccgaaggcaa gatcaggcag cgcgagactg tcatcgaagg ctttgaaaat
      361 ctgccgaaag cgctggtgct gcttttcgaa ggtgccaaca ccggcaagat gatggtgaag
      421 gtctagcgcc gctcagccgt cgccggtcgt tcctcccggc cccggcaccc gggcgtcgac
      481 gtttcagtgc agtgatcctc ccgcggagcg caccgtctcg acgtgctgct cgatctgggc
      541 gagcacgtcg tccagggggg cggtggtcag cctcagaatc atccgctcca ctcccagctc
      601 ggcgaacgca tcgaacgtgg cggcatccag gttcatgccc gagaccgtaa tggcgggggt
      661 ggggcgcccg gctgcgtccg ccatgcgttc cagctcgccg gccatctcca cgaactcgtc
      721 catgggcgtc acgcgcccct tcatctgctc cggtgccacg ggcgtgacca cgggcagcca
      781 gccttcgcca taggcgatca cccgcttgag cacgttgggc ccggccccgg caacgatgac
      841 gggcgggtgg ggttgctgca cgggtttcgg ccactgccag gtgggctcga tgttcacgag
      901 gcgcccgtgg tactcggcga cctcgttggt ccacagggct ttcatggcct ccacccgctc
      961 ccgcatcagg cggaagcgcg tgtttggatc cgtgccgtgg ttgcgcatct ccggctcgtt
     1021 ccatcccgcg ccgatgccga acagcatgcg cccgccggag agctggtcga tgctggccac
     1081 gagcttcgcg cagttgatgg gatcccgctg agtcaccagg cagatggccg tgccgagatt
     1141 gatgcgctcg gtgaccgtcg ccgcggcggc cagggcgacg aagggatcca gcatgctgga
     1201 gtactcgcgg ggcacggtat ccgacatcgg aaagtccgtg tccacaggga tgttggagtg
     1261 ctcggacacc cagaacgact cgaacccgtt tgcctccgcg gcctttgcga cttcggtggg
     1321 gctcgcgctg tagtccgtgg tgaacatgct caggccgaat ttcatggcgt tggtctcctt
     1381 gtcggaagcg tgcccgccga acggtcagag ggttgctgcc agggcgtagg cctgccgcgt
     1441 ggcaaaccag atcctccgcg gcgcgtaggc gtcgccgaga acgtgcagct cgtcgacctt
     1501 gcctttgagc gagtggtaca gcccgctgtc cgcaacgccg ccgcaggcga gcaccacgga
     1561 gtcgaagtcg gtgatctccg agccggcgcc ggagtagacg ttggcggtct cgatccggcc
     1621 ctgctcgatg ctggtgaccc gctccatcac cctcaccctg gcgccggatg cggtgatctt
     1681 ggccaggggc gcgccgatgg agtacttgcc gatgagcggc gcgatgctcg gcgtctggta
     1741 gatgagctgc acctcgtggc cccgatcggc caggaagctg gcaaccgtga ggggctgcag
     1801 atggtcctcc atggcgatga cggccacccg cttgcccggc ttggcctctc ccgacatcac
     1861 gtcgcggccc tccaggacga agggcaggtg ggcgcccgag gcttcatggc cgcggggcgt
     1921 ggcgccggtg gcaatggcca ccacgtcgaa gcctgcctcg gcgatggatt cggcggtggc
     1981 ctcccggccg gtctccacgg tgacgcccag ccgggcaagg cggcgctcct gaaattcgat
     2041 gaagtctccg aaggcccggt tctcgggcac ctgggcggca aggcgcatct gcccgccaag
     2101 ggcgtcgtcg cgctcccaca gggtgacggt gtgaccgcgc tcggcggcag cggctgccag
     2161 ctccaggccg gccggccccc cgcccaccac cagcaggttc ttaggcgggc tcgccggcgc
     2221 cggcggcggc ttgtgctcgt agcccgtgcg ggggttgacc gtgcagccga atggcaggcc
     2281 ctctaccacg cccgcgtgca ggcagtcgtt gcagccgatg cagggacgga tctcgtccag
     2341 acgcccggcc ttcgacttgg agacgatct
//
LOCUS       DBNHOX010000521         5192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1455251, whole genome shotgun sequence.
ACCESSION   DBNHOX010000521 DBNHOX010000000
VERSION     DBNHOX010000521.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1455251"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="AC16UZ_11360"
     CDS             <1..1165
                     /locus_tag="AC16UZ_11360"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6247326.1"
                     /translation="GLYCTLEDGADYGVPQWVFGIRQFAAVSDRHVVAARQGTETELV
                     MVDTEGGFATPLTDAGGAASLGSVVKWQDSVCCLAEFPDRLAAIVRCPLDGSPPTAIH
                     QPGTAPLGAELISRAERVQYPTRDGNRAFAYYYPPRNPEHPVTGAEGELPPLLVMSHG
                     GPTAAASRALNLRIQYYTTRGWAVVDVDYRGSSGYGRAYREALNGRWGVLDVTDCEDA
                     VRHLVDQGLADPDRIAIRGGSAGGFTTLAALTRSSTFKAGASHYGIGDLNALARDTHK
                     FESRYLHTLIGSEAALDERSPISHADALSAPVIFFQGSDDRVVPPAQSRQMADALRVR
                     GIPVAYVEFAGEGHGFRRAENIARSLENEHQFFCRVFGLIGDDDVKDLEIENL"
     gene            1162..2121
                     /locus_tag="AC16UZ_11365"
     CDS             1162..2121
                     /locus_tag="AC16UZ_11365"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR6247327.1"
                     /translation="MSHFELHVAVLMGGPSAEAEVSRVSAAEVAKGLDQAGHRATLLE
                     LDTGAAQALADLTPDVVFPALHGPPGEDGTVQGFLEILNLPYVGSGVHGSAVAMDKSL
                     AKAVFRRAGLPVADDLVVSVGADLAAAEREILDTLGERVVIKPLNQGSAIGVIRVPNG
                     GDLQAALQTALAFGHGVLVEPYVHGREITVGVLDVTGDEPEPFPVIEIITASDEWYDY
                     TNRYTVGKSEHVVPADVPDPVADALREHAVRAHAELGLRDLSRADYILTDANEIVLLE
                     VNTLPGMTPTSLFPDGARATGRDFPTLVDLLVRRAHQRGADGA"
     gene            complement(2170..3447)
                     /locus_tag="AC16UZ_11370"
     CDS             complement(2170..3447)
                     /locus_tag="AC16UZ_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007942367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR6247328.1"
                     /translation="MKKLMLGLAVLCVSAPAFADDLSQGNTAWILTSTALVLFMTIPG
                     LSLFYCGLVRTKNVLSVLMQCFVITCFVSILWLLVGYSLAFDADGGNAIIGGLGKVLF
                     AGVSESSMSGDIPETAFALFQLTFAIITPALIVGAFAERMRFSAMLMFSGAWLLLVYA
                     PICHWVWGGGWLGDLGLLDFAGGTVVHITAGVAAVVAALTLGSRRGFPQQAMPPHNMT
                     LTIAGAGMLWVGWFGFNGGSALAANGDAAMAMLVTHLSASAGAFTWMCAEWVRYGKPS
                     ALGAVTGMVAGLGTITPASGFVGPAGALVIGILAGIFCFAATNYLKRSLKIDDSLDVF
                     PVHGVGGILGTLLTAVFASSALGVFSGQEDIVILDQLGVQAIGVVATMALTGIATFVI
                     LKVTDALVGNRVSDEEEVEGLDLVLHNERGYDL"
     gene            3694..4674
                     /locus_tag="AC16UZ_11375"
     CDS             3694..4674
                     /locus_tag="AC16UZ_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6247329.1"
                     /translation="MQNTEAAVSVEQEKVTTVTDWLSKRIIGQETLLQRLMTALLCGG
                     HLLVEGAPGLAKTRAIKELASAVQGDFHRIQFTPDLLPSDITGTEVYRPEDGSFKFQH
                     GPVFHNLLLADEINRAPAKVQSALLEAMAEHQVSVGRQTFPLPELFLVMATQNPIEQE
                     GTYPLPEAQLDRFLMHVTVSYPSESAETSILHMVRKETGHRDEAATPPFRLTEADIFD
                     ARRAILGLHMAEPVERYIVALIMASREARGELGQWLEFGASPRGTIALDQCARALAWL
                     ERRDYVTPDDVRAIAHDVLRHRLILTFDADAQGVTTDQVIDQLLAEVPVP"
     gene            4737..>5192
                     /locus_tag="AC16UZ_11380"
     CDS             4737..>5192
                     /locus_tag="AC16UZ_11380"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6247330.1"
                     /translation="MDLGDLLSIRYRPAHSKTRPARRVSGLAGNRLSKLRGRGIDFSE
                     VRAYQPGDDVRTIDWRVTARKNKPHTKVFREERERLTLVFVDQSQGMFFGSRARLKSV
                     AAAEVAATAAWQALAHNDRVGGIVLGNRDAALHKPRRSVKNVARLLSDLA"
BASE COUNT          954 a         1736 c         1664 g          838 t
ORIGIN      
        1 cgggctttac tgcacgctgg aggacggtgc ggactacggc gtgccccagt gggtgttcgg
       61 cattcgtcag ttcgccgcgg tgagcgaccg gcatgtcgtc gccgcccgcc agggcaccga
      121 gacggaactc gtcatggtcg acaccgaggg cgggttcgcc acgccgttga cggacgccgg
      181 cggtgctgcg agcctgggct cggtggtgaa atggcaagac agcgtctgct gcctcgccga
      241 gtttcccgac cggcttgccg cgatcgtgcg ctgcccgctc gacggctcgc ccccgacggc
      301 cattcaccag ccgggcacgg cgccgttggg cgccgagctt atctcccgcg ccgagcgggt
      361 tcagtacccg acccgcgacg gcaacagggc cttcgcctat tactatccac ctcgaaatcc
      421 cgaacacccg gttacgggcg ccgaggggga acttccgccg cttctcgtca tgagccacgg
      481 tggccccacg gcagcggcgt cccgagcgtt gaacctgaga attcagtact acacaacccg
      541 cggctgggcg gtggtcgacg tggactaccg cggcagctcc ggatacgggc gagcctaccg
      601 agaagcgctc aacggtcgct ggggcgtgct ggacgtcacc gactgcgaag acgcggtccg
      661 ccacctggtc gaccaggggc tcgcagaccc ggaccggatc gccattcgcg gaggcagcgc
      721 tggggggttc accacgctgg cggcgctgac tcgctcgagc actttcaagg ccggcgccag
      781 ccattacggg atcggcgatc ttaacgcgct ggccagggat acgcacaagt tcgaatcacg
      841 ctacctgcac accctcatcg gcagcgaggc agctctggat gaacgctcgc ccatctccca
      901 tgcggatgcg ctgtcggctc cggtcatctt ctttcagggc agcgacgacc gggtggtccc
      961 gcccgcacag tccaggcaaa tggccgacgc gctgcgcgtg cggggcattc cggtggccta
     1021 cgtcgagttt gccggcgaag gccacggctt ccgacgggcc gaaaacatcg ccaggtcgct
     1081 cgagaacgag caccagttct tctgccgcgt attcggtctc attggtgatg acgacgtaaa
     1141 ggatttggag atagagaacc tgtgagccat ttcgaactgc acgttgccgt tctcatgggc
     1201 ggaccatctg ccgaagccga agtttccagg gtatccgccg cggaggtcgc aaagggcttg
     1261 gatcaggccg gtcaccgggc aacccttctt gaactggata ccggagcggc gcaggccctc
     1321 gccgacctga ctccggacgt ggtgtttccc gctcttcacg gcccgccggg ggaagatggc
     1381 accgttcagg gcttcctgga aattctcaat ctgccctacg tcggcagcgg cgttcacggc
     1441 agcgccgtgg ccatggacaa aagcctggcc aaggccgtgt ttcgccgtgc gggcctgccg
     1501 gtggccgacg acctcgtcgt gagcgtcggg gccgatctgg cggccgccga gcgggagatt
     1561 ctggacacgc tcggggagcg cgtagtgata aagcctctga atcagggctc cgccatcggc
     1621 gtgatccgcg tgccgaacgg cggcgatctg caggccgcgc tgcaaacggc cctggcgttc
     1681 gggcatgggg tcctcgtcga gccctacgtc catggccgcg agatcacggt cggcgtgctg
     1741 gacgtcaccg gggacgagcc cgagccgttt cccgtgatcg agatcatcac cgccagcgac
     1801 gagtggtacg actacaccaa ccgctacacc gtgggaaaga gcgagcacgt ggttccagcc
     1861 gacgtgccgg acccggtggc cgacgctctg agagagcatg ccgttcgcgc ccacgcggag
     1921 cttggcttga gggacctgtc gagagccgat tacatcctca cggacgccaa cgagatcgtc
     1981 ctcctggagg tgaacacgct gcctgggatg acgccgacca gccttttccc ggacggcgcc
     2041 cgggccaccg gaagggattt ccccacgctg gtcgacctcc tggtccgcag ggcccaccag
     2101 cggggcgcag acggagcctg accgccccct tgggaaatga ccctgaaaac ttagatagtt
     2161 ttcagggtgt tacaggtcat atccacgttc gttgtgaagg acgagatcga gcccttccac
     2221 ctcttcctcg tcggagacgc ggttcccgac cagcgcgtcg gtcaccttga gaatcacgaa
     2281 cgtcgctatg cccgtcaacg ccatggtggc gaccacgccg atggcctgaa cgccgagctg
     2341 gtcgaggatc acgatgtcct cctgcccgct gaagacgccc agggcgctgc tggcgaaaac
     2401 ggccgtgagc agcgttccca gaatcccgcc gaccccgtgg acggggaaca cgtccagcga
     2461 gtcgtcgatc ttcaatgacc gcttgaggta gttggttgca gcgaagcaga atatcccggc
     2521 gagaatgccg atgacgagcg cgccggcggg accgacgaag cccgatgccg gcgtgatggt
     2581 cccgagcccg gcgaccatac ccgtcacggc gcccagcgcg ctgggcttgc cgtatctgac
     2641 ccactcggcg cacatccagg tgaacgcgcc ggcggacgcg gacagatggg tgaccagcat
     2701 ggccatcgca gcgtcgccgt tggctgccag ggcagacccg ccgttgaatc cgaaccaccc
     2761 cacccagagc atcccggccc ccgcaatggt gagcgtcatg ttgtgcggcg gcatggcctg
     2821 ctgcgggaaa ccgcggcggg acccgagcgt gagcgctgcg accaccgcgg caacgccggc
     2881 ggtgatgtgg accacggtgc cgccggcaaa atccaggagc ccgaggtcgc cgagccagcc
     2941 gccgccccag acccagtgac atatcggagc gtatacgagc agcagccaag caccgctgaa
     3001 catcagcatc gcagagaagc gcatccgctc ggcgaaggcg cccacgatga gcgcgggggt
     3061 gatgattgca aaggtaagct ggaagagcgc gaaggccgtc tccgggatgt ctcccgacat
     3121 ggaactctca gacaccccgg cgaacaggac cttgcccagc ccgccgatga tggcgttgcc
     3181 gccgtcggcg tcgaacgcca ggctatatcc caccagcaac cagaggatgg acacgaagca
     3241 cgtgatgacg aagcactgca tgagcacgct gagcacgttc ttggtccgga cgagcccgca
     3301 gtaaaagagg gaaagaccag gaatggtcat gaacagaacc agcgccgtcg acgtgaggat
     3361 ccaggccgtg ttgccctggc tcaggtcgtc agcgaacgcg ggcgccgaca cgcacagcac
     3421 ggccaacccc aacatcagtt ttttcatcga tagtcccctc ttatgtcgcc acccgcccgg
     3481 ctttctgtgt ctccggttcc cggatgcccg cacaccgctt gatttcatca acgaatttac
     3541 ttcgatttcg ccaggggtgc cagaggcgat ccgcgtgctg ctcgattgac gcggaccgga
     3601 acgccggcgt cgaggatgta tttcgttgcc ggaaggctac aatacccggc ccggacgccc
     3661 cggcgggctt gcccgggcgc gcatgaggac gatatgcaga acacggaagc agcagtttcg
     3721 gtcgaacagg agaaggtcac tacggtgacc gactggctat ctaagcgcat catcgggcag
     3781 gagaccctgc tccagcgtct tatgaccgcg ctcctttgcg gcggccatct gctggtagaa
     3841 ggcgcgccgg ggctggccaa gacccgcgcc atcaaggaac ttgcaagcgc cgttcagggc
     3901 gattttcatc gcatccagtt cacgcccgat ctgctgccca gcgacatcac cggaaccgag
     3961 gtgtaccgcc cggaggacgg ctcgttcaag tttcagcacg gtcccgtttt ccacaatctg
     4021 ctgcttgccg acgagatcaa ccgtgccccg gcgaaggttc aatcggcgtt gctggaggcg
     4081 atggccgagc atcaggtcag cgttgggcgt cagacgttcc ccctgcctga actttttctc
     4141 gtcatggcga cccagaaccc catcgagcag gagggcacct acccgctgcc cgaggcccag
     4201 ctcgaccgct tcctgatgca cgtgacggtc agctacccct cggaatccgc ggagacgtcc
     4261 attctccaca tggtccgaaa ggaaaccgga cacagggacg aggcggcaac accgcccttc
     4321 cggctgaccg aagccgacat cttcgacgcg cgccgcgcga tactcggtct gcacatggcc
     4381 gaacccgtgg agcgctacat cgttgcgctg atcatggcgt cgcgggaagc gcgcggagag
     4441 ctgggccaat ggctggaatt cggcgcaagc ccacggggca ccatcgcgct ggaccagtgt
     4501 gcccgggcac tggcctggct ggaacgcaga gactacgtca cacccgacga cgtgcgcgcg
     4561 atcgctcacg acgttctgcg gcatcggttg atcctcacct tcgacgcgga cgcccagggc
     4621 gtcacgacgg accaggtcat cgaccagttg ctcgccgagg tgccggttcc ctgagctcgc
     4681 cgactgctag cacgatcctc ggctcgggtc gggaagaaat cctgaccggc gcctacgtgg
     4741 acctcggcga ccttctgtcc atacgctacc ggccggccca ctcgaaaacg cgaccggcgc
     4801 ggcgggtcag cgggctggcg ggcaaccgcc tgtccaagct gcggggccgg ggcatcgact
     4861 tctccgaggt gcgtgcctac cagcctggcg acgatgtacg caccatcgac tggcgggtca
     4921 ctgcgcgcaa aaacaagcct cacaccaagg tatttcgaga ggaacgcgaa aggctcaccc
     4981 tggttttcgt cgatcagagt cagggcatgt tcttcggcag ccgcgcccgg ctcaaatccg
     5041 tcgctgccgc cgaggttgcc gccaccgccg cctggcaggc gctcgctcac aacgatcgcg
     5101 tcggcggcat cgtgctgggc aaccgggacg ccgccctcca caaaccccga cgaagcgtca
     5161 agaacgtggc ccgcctgctg tccgatctgg cc
//
LOCUS       DBNHOX010000522         4517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_493127, whole genome shotgun sequence.
ACCESSION   DBNHOX010000522 DBNHOX010000000
VERSION     DBNHOX010000522.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_493127"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="AC16UZ_11385"
     CDS             <1..180
                     /locus_tag="AC16UZ_11385"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the transamination of the branched-chain
                     amino acids to their respective alpha-keto acids; Derived
                     by automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HIR6247331.1"
                     /translation="AANEAFLTNTSTGVWPIERVDDVVFGDGAPGPVTKRLRAALRKI
                     SSGEEPTFRHWLHYV"
     gene            complement(216..719)
                     /locus_tag="AC16UZ_11390"
     CDS             complement(216..719)
                     /locus_tag="AC16UZ_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247332.1"
                     /translation="MATRRRTLAMGLALLAGGYLGPLARHARAQSLEQAMEESDLIYL
                     TPLKSDGSESRCRAEVWFARHEADLFVVTSSDAWRARAVERGLTRTRVWVGDVGVWSG
                     SGGSYRTLPQIEADASIVAEADVHDAVLDVMGDKYRMSWLVWGPRFRNGLADGSRVML
                     RYRPAAA"
     gene            complement(754..2274)
                     /locus_tag="AC16UZ_11395"
     CDS             complement(754..2274)
                     /locus_tag="AC16UZ_11395"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011335679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HIR6247333.1"
                     /translation="MTIIREDDLIESVADALQFISYYHPLDFIRAMRSAYEKEESPAA
                     KAALTQVLVNSRMCAIGKRPICQDTGIVNVFLEVGMEVSWSGKRSVEEMINEGVRRAY
                     MHPDNVLRASVLTDPDGARSNSRDNTPAVIHTRIVPGDKLSVEVAAKGGGSEAKAKFA
                     MLNPSDSIVDWVLDVVPTMGAGWCPPGLLGVGIGGTPEKAMLLAKEALMEPIDIHDLK
                     ARGPANHLEELRLELFEKVNALGIGAQGLGGLTTVLDVKVKDYPTHAANKPVAVIPNC
                     TATRHVHFTLDGSGPAVFEAPDLDEWPDIAFELGDDVRRVDMDTLTPDDVRNWKAGDT
                     LLLTGKMLTGRDAAHKRLADLIERGEPLPVDFTNRIIYYVGPVDPVRDEVVGPAGPTT
                     ATRMDKFTGMMLEKTGLIGMIGKAERGPAGVDAIREHGAVYMIAVGGAAYLVAKAITR
                     STPLAFEDMGMEAIYEFEVKDMPVTVAVDSTGASVHREGPRLWSQKIADRIGAAQL"
     gene            2369..3625
                     /locus_tag="AC16UZ_11400"
     CDS             2369..3625
                     /locus_tag="AC16UZ_11400"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR6247334.1"
                     /translation="MRRSRILILLSTVAAGCGEPGVSPDLEHARALSRSELIVDTHID
                     LPYRLYRRQEDVAEPTSGEFDYPRALAGGLDAAFMSIYVPAAVDAEGRATEMADRLID
                     LVEGIARDAPDKFAVATCTRDVEAIRASGRIALPMGMENGGPIAGDLGKLDHFMGRGV
                     RYITLAHSKSNHISDSSYDENEPWEGLSPFGRDLVTAMNQRGMMIDVSHLSDRAFWQV
                     LELSSAPVVATHSSLRHFVPGFHRNMSDEMVAALGRNGGVVQINFGSSFVTAEARAYS
                     DAMTAAVKAFAETEEPRYDDPAVRAFAASYRAEHPYPYAELEDVLNHVDRAVALAGIE
                     HVGLGSDYDGVGDTLPVGLKDVSSYPNLIAGLADRGYDDHSISLILGGNLMRVWREVE
                     AVAAAQGFPTACRHDAPARSALSQRL"
     gene            complement(3633..4391)
                     /locus_tag="AC16UZ_11405"
     CDS             complement(3633..4391)
                     /locus_tag="AC16UZ_11405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HIR6247335.1"
                     /translation="MKIADVARLHRQWSYGHELGFPALLLRSEAGIDGWRLDMTARRP
                     SKGFSLIELMVVVGILAVLAAFAMPAYLDYLESANTSKVLSHYEDGTRYLRTEILRLN
                     TSVVMGKETLESIDANFTSAFWVDKLNQEGGRAPGGGPAYGASVDHGMGRVGVVVAGS
                     LLDAPPSYRLEVTRPQYGGFAQYSVMTAIISWSGGGGGTSFGSQADPTPGQPGGLDGS
                     GPGQGAGSADNPGQGKDQGQDKGKGQGQGQGQNV"
BASE COUNT          800 a         1532 c         1370 g          815 t
ORIGIN      
        1 gcggccaacg aggcgttcct caccaatact tccacgggcg tgtggcccat cgagcgcgtt
       61 gacgacgtgg tgttcggcga cggggcgccc ggcccggtga ccaagagact acgtgccgcc
      121 ctcagaaaga tctcgtccgg cgaggagccc accttccgcc actggctgca ctacgtctga
      181 cctcacggcc agaagcggtc gatgttcgtg gtcggtcagg cggcagcagg acggtaccgg
      241 agcatgaccc gcgacccgtc cgcgagaccg ttcctgaatc ggggacccca gaccagccag
      301 gacatccggt acttgtcacc catgacgtcg agcaccgcgt cgtgcacgtc ggcctccgcc
      361 acgatcgagg catccgcttc gatctgcggc aacgttcggt agctgccgcc gctgccggac
      421 cagacgccca cgtcgccaac ccaaacccgg gtgcgcgtga gccctcgctc gacggctctc
      481 gcccgccagg cgtcgctcga cgtgacgacg aagagatcgg cttcatgacg cgcgaaccag
      541 acctccgccc ggcagcggct ctccgagcca tcgctcttga gaggagtcag gtagatcagg
      601 tccgattcct ccatcgcctg ttccagcgac tgcgcccggg cgtgacgggc gagagggccg
      661 aggtatcccc cggcgagcag cgccaggccc atcgccagcg ttctccttct tgttgccatc
      721 acacagcctc cgccggatag tgaagtcggg tcgtcacaac tgggcagcgc ctatgcgatc
      781 ggcgattttc tgcgaccaca gcctgggtcc ttcgcgatgc accgatgcgc cggtcgaatc
      841 caccgccacc gtgaccggca tgtctttgac ctcgaactcg tagatggcct ccatccccat
      901 gtcctcgaag gccagcgggg tgctccttgt gatcgctttg gcgacgaggt aagcggcgcc
      961 acccacggcg atcatgtaaa ccgcgccgtg ctcgcggatg gcatcgaccc cggccggacc
     1021 cctctcggcc ttgccgatca tgccgatcag ccccgtcttc tccagcatca tgccggtgaa
     1081 cttgtccatg cgcgttgccg ttgtcggccc cgcggggccc acgacctcgt cgcgtactgg
     1141 gtccacgggg cccacgtagt agatgatgcg attggtgaaa tccacgggta gaggctcgcc
     1201 acgctcgatg aggtccgcca gccgcttgtg ggcggcgtcc cggcccgtca gcatcttgcc
     1261 ggtgagcagc agcgtatctc ccgccttcca gttgcgcaca tcgtccggcg tcagcgtatc
     1321 catgtcgacg cgccgcacgt cgtcgcccag ctcgaaagcg atgtccggcc actcgtccag
     1381 atccggcgcc tcgaagacag cgggcccgga tccgtccagg gtgaaatgca cgtgacgcgt
     1441 cgccgtgcag ttggggatca cggccaccgg cttgttggcc gcatgggtcg ggtagtcctt
     1501 caccttgacg tcgagtacgg tggtcagccc accgagcccc tgagccccga taccaagcgc
     1561 gttgaccttc tcgaagagct cgagacgcag ctcttctagg tggttggcgg gaccgcgggc
     1621 cttgagatcg tgaatgtcga tgggctccat gagcgcttcc ttggcgagca gcatggcctt
     1681 ttccggcgtc ccgccgatgc cgaccccgag cagccccggc gggcaccagc cggcgcccat
     1741 ggtcggcacc acgtccagca cccagtcgac gatggagtca gacgggttga gcatggcgaa
     1801 cttcgccttg gcctcgctgc caccaccctt cgcggccacc tccacggaaa gcttgtcacc
     1861 gggaacgatc cgggtgtgga tcaccgccgg cgtgttgtcc ctggaattgc tgcgcgctcc
     1921 gtccgggtcg gtcagcaccg acgcgcgcag cacgttgtcc gggtgcatgt aggcgcgacg
     1981 gactccctcg ttgatcatct cctcgacgct tcgcttgccc gaccacgaga cctccatgcc
     2041 gacctcgagg aacacgttga cgatgcccgt gtcctggcag atcggccgct tgccgatggc
     2101 acacatgcgc gagttcacca gaacctgggt cagcgccgcc ttggccgccg gagactcttc
     2161 tttctcatat gcgctgcgca tggcgcggat gaagtccagc ggatggtagt aggagatgaa
     2221 ctgcagcgcg tcggcgacgc tttcaatgag gtcatcctca cgaatgatgg tcatcttgat
     2281 tcctgcccgg ttgcagagcg cattttaact cagcttgtgc tgcgcggttc gggcgtgtga
     2341 acataatccc tccgcggaga tggctcacat gcgacgctcg agaattctga ttctattgtc
     2401 caccgtcgcc gccggctgcg gagagcccgg agtgagcccg gacctggagc acgcgcgagc
     2461 cctctcccgc agcgagctca tcgtagatac ccacatcgac ctgccctacc ggctttaccg
     2521 gcgccaggaa gacgtcgccg agcccacgag cggcgagttc gattacccca gggccctcgc
     2581 cggaggactg gacgcggcgt tcatgtccat ctatgtcccc gccgccgtcg atgccgaagg
     2641 acgcgcaacc gagatggccg atcgtctcat cgacctggtg gagggcatcg cccgcgacgc
     2701 gccggacaag ttcgccgtgg caacctgtac ccgagacgtg gaggccattc gcgcgtcggg
     2761 acgcatcgct ctgccaatgg gcatggagaa cggcggcccc attgccggcg atctgggcaa
     2821 gctcgatcac ttcatgggcc gcggcgtccg ctacatcacg ctggcgcact cgaagtcgaa
     2881 tcacatctcg gattcctcgt acgacgagaa cgagccctgg gagggcctgt cgcccttcgg
     2941 cagagacctg gtaacggcga tgaaccagcg cggcatgatg atcgatgtgt cccacctcag
     3001 tgaccgcgct ttctggcagg tgctggagct ctcgagcgcg ccggtggtgg caacgcactc
     3061 ctctctgcgc catttcgtgc cgggctttca ccgcaacatg agtgacgaga tggtggccgc
     3121 gctgggacgc aacggcggcg tcgttcagat caatttcggc agcagcttcg tcaccgccga
     3181 agcccgggcg tactcggacg ccatgaccgc ggccgtgaaa gcattcgccg aaaccgagga
     3241 gccgcgctac gacgatccgg ccgtgcgggc gttcgccgca agctaccgcg ccgagcaccc
     3301 gtatccctac gcggagctcg aagacgtcct gaatcacgtc gaccgcgccg ttgcgctggc
     3361 cggcatcgag cacgtcggcc tcggttccga ctacgatggc gtgggcgaca ccctgccggt
     3421 gggtctgaag gacgtcagct cgtacccgaa cctgattgcc ggcctggccg accgggggta
     3481 tgacgaccac agcatctccc tgattctcgg cggcaacctg atgcgcgtgt ggagggaagt
     3541 ggaggccgtt gccgccgccc agggatttcc gacagcctgc cgccacgacg ctccggcgag
     3601 atcggcgctc agccagcggc tgtaaagcca ccctaaacgt tttggccctg accctggcct
     3661 tgacctttgc ctttgtcttg cccctgatct ttgccttggc caggattgtc tgcgcttcct
     3721 gcgccctggc ccgggccgga accatccaag ccgccaggct ggccaggggt gggatcagcc
     3781 tgcgagccaa acgatgttcc cccgccgcca ccactccagg aaatgatcgc ggtcatcacc
     3841 gagtactgtg cgaacccgcc gtactgggga cgagtcacct cgagtcgata ggatggcgga
     3901 gcgtccaaca aactaccagc gaccaccaca cccacacggc ccatgccgtg atcgacggag
     3961 gctccgtaag cagggccccc tccgggagcg cgccctcctt cctgattcag cttgtcgacc
     4021 cagaatgcag acgtgaagtt cgcgtcaatg gattcgaggg tttcttttcc catgacgacc
     4081 gacgtgttca gcctgagaat ttccgttctg aggtagcgcg tgccatcctc gtaatgagaa
     4141 agaaccttgc tggtgttggc gctctccaag tagtcaagat aagcgggcat ggcgaaggct
     4201 gccaaaacgg cgagaatacc caccaccacc atgagttcaa tcagcgaaaa gcctttcgag
     4261 ggccgccgcg ccgtcatatc gagtcgccat ccatctatac cggcctccga tcggagcagg
     4321 agcgccggga aacccaattc atgtccgtat gaccactgac gatgcagcct cgcaacgtcg
     4381 gcaatcttca cctatgcgct ggcaaatgct acacaaatca gttcacaaat cgattgacag
     4441 acgtctcacg gttatcgtgc ctcggggccg aatttctaat caagaaagct gctcgatcaa
     4501 gcgtcgcgca aacgtcg
//
LOCUS       DBNHOX010000523         5720 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_876919, whole genome shotgun sequence.
ACCESSION   DBNHOX010000523 DBNHOX010000000
VERSION     DBNHOX010000523.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_876919"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..158)
                     /locus_tag="AC16UZ_11410"
     tRNA            complement(83..158)
                     /locus_tag="AC16UZ_11410"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(123..125),aa:His,seq:gtg)
     gene            complement(232..308)
                     /locus_tag="AC16UZ_11415"
     tRNA            complement(232..308)
                     /locus_tag="AC16UZ_11415"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(272..274),aa:Arg,seq:tct)
     gene            complement(325..401)
                     /locus_tag="AC16UZ_11420"
     tRNA            complement(325..401)
                     /locus_tag="AC16UZ_11420"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(365..367),aa:Pro,seq:tgg)
     gene            574..1425
                     /gene="folD"
                     /locus_tag="AC16UZ_11425"
     CDS             574..1425
                     /gene="folD"
                     /locus_tag="AC16UZ_11425"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR6247336.1"
                     /translation="MTASILDGKSLAKSLRADLAAAVQERLESGGRPPGLAVILVGND
                     PASEVYVRHKSRDCEEVGFHSVVQHLSENVTQQDLEERIVALNANPSIDGILVQLPLP
                     AHIDSHRIIDRIDPLKDVDGFHPYNIGRLALRRPVLRPGTPKGIMQLLEVTGVPIRGL
                     DATILGASNHVGRPMGLELLLAGCTVTTAHKFSRDARASARSADILVSAVGKPGLVGG
                     DWIKPGAIVIDVGITRGEDGKLRGDVVFDEAMEVASWVTPVPGGVGPMTRVAMLQNTL
                     AAADGSD"
     gene            1430..2146
                     /locus_tag="AC16UZ_11430"
     CDS             1430..2146
                     /locus_tag="AC16UZ_11430"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HIR6247337.1"
                     /translation="MHRIYISDLHLSDESEPQFATLAALLRHEAPRADAIFILGDLCE
                     AWVGDDDDSPLATALRAALKYAGEQTSLHVMRGNRDFLLGERFAAEIGCCLVEDPCLI
                     DPDTLIAHGDAFCIDDEAYQATRRVLRSEAWQQDVLARPLEERRGLAAQMREQSRLSN
                     ANKAENIMDVSADEVDRTVKEYGVRRLIHGHTHRPGVHPGDWGTRYVLGSWEHCGWLL
                     REQDTVTALECFPLSGRYGT"
     gene            complement(2100..2738)
                     /locus_tag="AC16UZ_11435"
     CDS             complement(2100..2738)
                     /locus_tag="AC16UZ_11435"
                     /EC_number="1.14.99.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 - tRNA
                     2-(methylsulfanyl)-N(6)-isopentenyladenosine(37)
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaE family protein"
                     /protein_id="HIR6247338.1"
                     /translation="MSATRSRAKPEDAAEFLKCSTPVLWFEQAAGNLETLLVDHANCE
                     KKAAGTALSLIYRYVDRPELLQRLSRLAREELRHFEQVHALMCSMGIAYHALAPGRYA
                     ASLMQQVRTHEPARLVDTLLIGAIVEARSCERFAGLVDVLPGGVGELYAALMVSEARH
                     FRGYLDLAQRYGDGSLEERLDHLLGFESELASGPDTQFRFHSGPIAGNIPAQ"
     gene            complement(2763..3209)
                     /locus_tag="AC16UZ_11440"
     CDS             complement(2763..3209)
                     /locus_tag="AC16UZ_11440"
                     /inference="COORDINATES: protein motif:HMM:NF018630.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HIR6247339.1"
                     /translation="MTATRRSTPCVGICSTTYGDLVCRGCKRFAHEIVSWNAFDVDQR
                     EAVWSRLEELRTGAIARCLRVLDEERLIAEAEAVRVPQAAQRELLDLAYEALRRWRDR
                     DAPPERLGVEALAGADTAQDLIRQIDREFYSRSLAHYERSFKIAAQ"
     gene            complement(3220..5241)
                     /locus_tag="AC16UZ_11445"
     CDS             complement(3220..5241)
                     /locus_tag="AC16UZ_11445"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF023418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase TgpA family protein"
                     /protein_id="HIR6247340.1"
                     /translation="MASARASSGVGFAYQIPRNSLALLMFAQVVVVLPFALQLSPWII
                     AVGLFCGYWRAGVYQGRWSYPHRWVKALLVLASVVGVAVSGAGAFSLEAAASLLILAF
                     ALKLIEMKSRRDAYLVIFLAYFVIATQFLFDQSILVAVYELGAICVVTAAMIGLNQLH
                     TRVRPLASLRDASKLVLQALPFTLVLFLLFPRIAPLWTVPLPTAGKTGITDVVKPGAI
                     AALTQSDELAFRVVFEGEIPRYRDLYWRGLVYSEFEDGAWSVGPELPAVPSSLRPEPG
                     RGEVAYEVLLEPTMSDWLFALDTAFPRSAGVASTQDYRLRARDPILSVFRYGVASYPD
                     FVMDERLTGRLITRETRIDLQDSPRAVALARSLHDGDDERFVDAVLELIRQEPFRYTL
                     NPPRLPDAGSIDAFWFESRAGFCTHYAGAFVYLMRSVGIPARMVGGYQGGEINPISNH
                     LMVRQYDAHAWAEVWLRGKGWQRVDPTAAVAPERIERGLSAALSANDRARLSALTSAR
                     LGAWSALADMLYWVDSLEHRWNLWVIGYDSRFQAGFLERLLGKVTPGRTGLVMLAGGV
                     ASIGLVAVFLFWRRRPRKRHPVERMFLRFAGKVSGYGLQRNPQESPGAFVRRAARNAG
                     WRDEQADQIVAQLNALLYNPAVSWGRAEVASLAAQLRRLQFRLAFASAR"
     gene            complement(5222..>5720)
                     /locus_tag="AC16UZ_11450"
     CDS             complement(5222..>5720)
                     /locus_tag="AC16UZ_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6247341.1"
                     /translation="NPGRLLVETYYPLGLLRAWTWVDLDARALVYPKPIFQDFPLSEQ
                     GRRDEGELIDPLGSEDFTDIKGYQPGDPIKHIIWRSYARSNDVMVKRYASYLEPRLWL
                     DLETVAGPLEERLSRLTGMALTATRLEREFGLRLGGLEIKPATGETHLEQVLRELALH
                     GIRES"
BASE COUNT         1074 a         1885 c         1842 g          919 t
ORIGIN      
        1 agcccggtca tggtttctat ggaaatttgc acgatcttcg cttgtctacc ttggaacttt
       61 ctaccgcgct agtcaggatc attggggtgg acgatgggga ttgaacccac gaccgccggg
      121 atcacaaccc ggagctctac caactgagct acgtccaccg tagcctggtc tttcacccga
      181 ccgctgggcg gcgccattct aaccgaacca tcgagccgct cgatacgagt ctggtacgcc
      241 cggaaggact cgaaccttca accctcggct tagaaggccg atgctctatc cagttgagct
      301 acgggcgcat tctcgatgag aaattggtcg gggtagagag atttgaactc ccgacatcct
      361 gctcccaaag caggcgcgct accagactgc gctatacccc gaagtggctg ttgcacaacc
      421 cgcgcccgaa gcggcgcgat gatactggtg gtgttctgag ccgtcaaacg cagacgccgg
      481 gccctgaacg ggtcgatcgg cggtcggggt tggccggtct gaggccggtt cgttgtaata
      541 tgggggccta caggcaagcg gaatccgggt gacgtgacag cctccatcct cgacggcaaa
      601 agtctggcga aatcgctcag agcagacctg gcggcggcgg tgcaggagcg gctcgaatcc
      661 ggcgggagac caccgggcct tgcggtcatt ctggtcggaa acgatccagc ctcggaggtc
      721 tacgtgcgtc acaagagtcg ggactgcgag gaagtcgggt tccactccgt ggtgcagcat
      781 ctgtccgaga acgtcaccca gcaggacctc gaggaacgca tcgttgccct caacgcaaac
      841 ccgtccatcg atggcatatt ggtccagcta ccgcttcccg cccacatcga cagtcaccgg
      901 atcatcgacc ggatcgaccc tctcaaggac gtggacggct ttcatcccta caacatcggc
      961 cgtctcgccc tgcgcagacc cgtgctgcga cctggcacgc caaaaggcat catgcagctc
     1021 ctggaggtca ccggtgtccc gattcgcggc ctggacgcaa ccattctcgg cgcctccaat
     1081 cacgtcggcc gaccgatggg cctggagctc ctgctggccg gatgcacggt gacgaccgcg
     1141 cacaaattct cccgcgacgc ccgggcctca gcccggtcgg ccgacatcct ggtgtctgcc
     1201 gtgggcaagc ccgggctcgt cgggggcgac tggatcaaac ccggcgccat cgtgatcgac
     1261 gtcggcatta cccggggtga ggacggcaag cttcggggag acgtggtgtt cgacgaggcg
     1321 atggaagtcg ccagctgggt cacgcccgtg cccggcggcg tggggcccat gacgcgggtg
     1381 gccatgcttc agaacaccct cgccgcagcc gacggatcgg actgaaccct tgcaccggat
     1441 ctacatctcg gaccttcatc tttcggatga aagcgagccg cagttcgcaa cgcttgcggc
     1501 tctgctgcgg cacgaggcgc ctcgcgccga cgccattttc attcttggcg acctgtgcga
     1561 agcgtgggtt ggtgacgacg acgactcacc gttggctacc gcgcttcgcg cagcgctgaa
     1621 gtacgccggc gagcagacca gtctgcacgt catgcgaggc aacagggact tcctgctcgg
     1681 cgagcggttc gcagcggaaa tcggatgctg cctggtcgag gatccctgtc tgatcgaccc
     1741 cgacaccctg atcgcccatg gggacgcctt ctgcatcgat gacgaggcct atcaggccac
     1801 gcgccgcgtt ctgcgctccg aggcgtggca gcaggacgtg ctggcacgcc cgctggagga
     1861 gcggcgcggg cttgctgccc agatgcgcga gcagagccgc ctgagcaacg ccaacaaggc
     1921 agagaacatc atggacgtct cggctgacga ggtggatcgg acggtcaagg agtacggggt
     1981 cagacggcta atacatggtc atacccatcg ccccggcgtt cacccggggg actggggaac
     2041 ccgttacgtg ctcggctcct gggagcattg cgggtggctg ctgcgcgagc aggacaccgt
     2101 cactgcgctg gaatgtttcc cgctatcggg ccgctatgga acctgaactg agtgtccgga
     2161 cctgacgcga gctcggactc gaagcccaac agatgatcga gcctctcctc cagcgaaccg
     2221 tcgccgtacc tctgcgctag atccaggtat cccctgaaat ggcgcgcttc cgagaccatc
     2281 agagccgcgt acagctcgcc gaccccgcca ggaagcacgt ccacgagccc ggcaaaccgc
     2341 tcacaggatc gggcctccac gatggcgcca atcaggaggg tgtcaacgag ccgggccggc
     2401 tcgtgggtcc tgacctgctg cataaggctg gccgcgtagc ggccgggagc cagcgcgtga
     2461 taggcgatgc ccatggagca catcagcgcg tgcacctgct cgaagtgacg aagctcctcc
     2521 cgggcgagac gggacagccg ctgcagcagc tcgggccgat ccacgtagcg atagatgaga
     2581 ctgagcgcgg tgcccgccgc cttcttctcg caattggcgt ggtcgacgag cagagtctcg
     2641 aggttcccgg ccgcctgctc gaaccacagc accggtgtcg agcacttgag aaactcggcg
     2701 gcatcctcgg gtttcgcgcg gcttcgggtg gcgctcacag cgtggcgctc acagcgtggc
     2761 gctcattgag cggcgatctt gaagctgcgc tcgtagtggg cgagcgaccg gctgtagaac
     2821 tcacggtcga tctggcgaat cagatcctgc gccgtgtctg ccccggcaag ggcctccacg
     2881 cccagccgct ccgggggcgc gtcgcgatcg cgccacctcc tgagcgcttc ataggccagg
     2941 tccagcagct ctctctgcgc ggcctggggg acccgcacgg cttcggcttc ggcgatcagc
     3001 cgctcctcgt cgaggacgcg caggcatcgg gcgatggcgc cggttcgcag ctcctcgaga
     3061 cggctccaga cggcctcacg ctggtccaca tcgaaggcgt tccagctgac gatctcgtgg
     3121 gcgaaccgct tgcacccgcg gcagaccagg tcgccgtagg tcgtcgagca tatgccgaca
     3181 cagggggtgc tgcggcgcgt ggccgtcatg acgatgtcgc tacctcgcag atgcaaaggc
     3241 caatctgaac tgaagccgcc ggagctgcgc ggcaagcgac gcgacctctg cgcggcccca
     3301 ggagacggcc ggattgtaca gaagcgcatt caattgggcg acgatctgat ccgcctgctc
     3361 gtcccgccat ccggcattgc gggctgcccg gcggacgaaa gcacccggcg actcttgggg
     3421 atttcgctgc aggccgtagc cggaaacttt tccggcaaag cgcaggaaca tgcgctcgac
     3481 cgggtgccgt ttgcgggggc gcctgcgcca gaagaggaag acggcgacga ggccgatgct
     3541 tgcgacaccg ccggcaagca tcaccagacc cgtgcggcct ggcgtgacct tgccgagcag
     3601 gcgctcgaga aatccggcct ggaagcggga atcgtagcca atcacccaga ggttccagcg
     3661 atgctccagc gaatctaccc agtacagcat gtccgccagg gcgctccagg cgccgagcct
     3721 tgcgctcgtc agcgccgaca gcctcgcgcg atcgttggcc gatagcgcag ccgagagccc
     3781 ccgctcgatg cgctccgggg ccacggccgc cgtgggatcg acgcgctgcc agcctttgcc
     3841 gcgcaaccaa acttccgccc aggcatgagc gtcgtactgc ctcaccatga ggtgattgct
     3901 gatcgggttg atctcaccgc cctgatagcc acccaccatg cgcgccggga tcccaacgga
     3961 ccgcatgaga tagacgaacg cccccgcgta gtgcgtgcag aagccggcgc gagactcgaa
     4021 ccagaacgca tcgatactgc ccgcgtcagg cagcctggga gggttcagcg tgtagcgaaa
     4081 gggctcctgg cgaatcagtt cgagcacggc gtcgacaaag cgctcatcgt cgccgtcgtg
     4141 cagcgatctg gccaacgcca ccgctctggg gctgtcctgg agatcgatcc tcgtttcacg
     4201 cgtgatcagg cggccggtca gccgctcgtc catcacgaag tccggatagg acgccacgcc
     4261 gtatcggaaa acggacagaa tcgggtcgcg cgcccgcagc cgataatcct gggtcgacgc
     4321 aacgccggcc gaacgcggaa atgcggtgtc cagggcgaaa agccagtcgc tcatggtcgg
     4381 ctcgagcaga acctcgtagg cgacttcgcc cctcccgggc tcgggcctga gcgagctcgg
     4441 cactgcgggc agctcgggcc cgacggacca ggcgccgtcc tcgaattccg agtagacgag
     4501 cccgcgccag tacaggtcgc gataacgcgg gatctcgcct tcgaacacga ccctgaacgc
     4561 cagctcgtcc gactgggtca gagcggcgat cgcgccgggc ttgaccacgt ccgtgatgcc
     4621 cgtcttcccc gcggtaggta gaggcaccgt ccacagcggc gcgatgcgcg gaaaaaggag
     4681 aaacagcacc agggtgaaag gcagcgcctg gagcaccagc ttggaggcgt cgcgcaacga
     4741 agccagcggg cgcacgcgcg tgtgcagctg gttcaggccg atcatggcgg ccgtgaccac
     4801 gcagatggcc cccagctcgt agacggccac caggatggac tgatcgaaga gaaactgcgt
     4861 ggcgatcacg aagtaagcga gaaagatcac cagataggcg tcgcggcggc tcttcatctc
     4921 gatgagcttg agagcgaacg ccaggatgag cagcgaagcc gccgcctcca ggctgaacgc
     4981 ccccgcaccg ctcacggcca cgccaaccac ggacgccagc accagcaagg ctttcaccca
     5041 tcgatgcgga tagctccacc ggccctggta gacgcccgcg cgccagtagc cgcagaacag
     5101 gccaacggca atgatccagg gggagagctg cagcgcaaag ggcaatacca ccacgacctg
     5161 cgcgaacatc agcagcgcca ggctgttgcg gggaatctgg taggcgaagc ctacgccgga
     5221 actagctctc gcggatgcca tggagggcca gctccctgag cacctgctcg agatgcgtct
     5281 cacctgtggc gggcttgatt tccaaccccc ccagcctcag accgaactcc cgctccaggc
     5341 gggtggccgt cagtgccatg cccgtgagtc ggctcaaacg ctcttccagc gggccggcga
     5401 cggtctccag atccagccag agcctgggct cgagatagct ggcataccgc ttcaccatga
     5461 cgtcgttcga tcgcgcgtac gagcgccaga tgatgtgctt tatcggatct ccgggctggt
     5521 agccctttat gtccgtgaag tcctcgctgc ccaggggatc gatgagctcg ccttcgtccc
     5581 ggcgcccctg ctccgaaaga gggaagtcct ggaagatggg cttgggataa acgagggccc
     5641 gggcgtcgag gtctacccac gtccacgccc gaagcaatcc gagggggtag taggtctcga
     5701 cgagcagccg cccggggttg
//
LOCUS       DBNHOX010000524         9976 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_493316, whole genome shotgun sequence.
ACCESSION   DBNHOX010000524 DBNHOX010000000
VERSION     DBNHOX010000524.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9976)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9976
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_493316"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="AC16UZ_11455"
     CDS             <1..834
                     /locus_tag="AC16UZ_11455"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6247342.1"
                     /translation="GCVYGLIALGFVLIYKATEMVNFAQGDVMMLGAFLAFALINLME
                     LPFLWGFMGALAAMAVVGVLLERLLLRPMIGEPHFAVLMLTIGLGFILRAVAGAIWGN
                     EPRALHTPYGGEVLRFGDLVVGYENIVIIVGTAVLCGLLFAFFRFTRVGVAMQAASQN
                     QLAAYYVGIPVKRIYSLVWALSATIAACAGVLIAPVTLIDPLMGSFLGIKAFAAAIVG
                     GFGSLPGAILGGLLVGITEQFVGLYFPPGFADVSAYVLLLVMLMIRPEGLFATMQRKK
                     V"
     gene            839..1915
                     /locus_tag="AC16UZ_11460"
     CDS             839..1915
                     /locus_tag="AC16UZ_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6247343.1"
                     /translation="MRFIRKTHYVQDVDLIKHPGQRFWYGLLAIVLVLAPLGLDRFYL
                     GELSLVFIYAIAGIGLMLLVGYTGLVSLGHAAFLAVGAYCHSWLLGNGVPLPLAMVAA
                     TLVTAGVGAVVGVPALRMTGIYLAVATLALAVIVEKVLEKWETVTGGLRGLPVPKAQF
                     LGFDLGAPANFYYLCLGVLALGLLAAMNLLRSPTGRAMVAVRDSETSAQSMGIHINRI
                     KGLAFALSAGFTGLAGALFAHRIGYLFPDSFTVLTSIQLLLMVVVGGLGSLHGVVFGA
                     IFIGLLPQGIALFRDVVPPAIAQIPGLEPGIFGLILVLFLIFEPLGIYGRWVKIRLFF
                     EEFPLYRRATYKRQKTYLRTERLR"
     gene            1912..2670
                     /locus_tag="AC16UZ_11465"
     CDS             1912..2670
                     /locus_tag="AC16UZ_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6247344.1"
                     /translation="MSLFAAEDVAVSFGGIKAVHDLSFAVEDGEVFSIVGPNGAGKTT
                     IFNLISRIYDVDQGSLAFDGTEITRLPAHAVAELGVARTFQNTELFEQETVLKNLLIG
                     CHVHRRSGLLADILFLPSVKAQELTFRKRVEDVIDLLDLQSYRDQVIGNLPYGVRKMV
                     EIARALCLGPKLLLLDEPSSGLNPEETEDLSFWLEDINNDLGITIIMVEHDMNLVSRA
                     SDRVMALADGALLTIGSPADVQQHPDVLRAYLGD"
     gene            2682..3440
                     /locus_tag="AC16UZ_11470"
     CDS             2682..3440
                     /locus_tag="AC16UZ_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6247345.1"
                     /translation="MLELKNVETYYGPIMAIRGVSMEVPEGKIVSLLGANGAGKTTVL
                     KTISGAMDPQKGTITYQGEPIQARDPDWVARRGIAHVPEGREVFPFLCVEDNLRMGAF
                     SRRDAEIGDDIELVYGYFPVLKELARQQAEYLSGGQQQMLAIGRALMLRPRLMLLDEP
                     SLGLSPRLVAEIAEIIVRLNREQAVTMLLVEQNAKMALDIGDYGYVMEIGRIVMEDTC
                     ERLKNATDIKEFYLGMKDEGVRGRRRWKQRKTWR"
     gene            3437..5251
                     /locus_tag="AC16UZ_11475"
     CDS             3437..5251
                     /locus_tag="AC16UZ_11475"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR6247346.1"
                     /translation="MSPQIIDGCATVARLFWKRATDLPDKIALREKDFGIWNEYTWGD
                     WGEQARLVGLGLKALGLKRGDVCSIATDVNKEWLFADHGVIGVGGIVNGVYPTDAPNQ
                     VEYLINDSRTRFYFAEDEEQLDKVLEVRGNTPTLEKIIIFDMEGLRTFSDEQCMSFDA
                     LLALGREYGEQHPGLWEQEIEAAEAEDLLILTYTSGTTGNPKGAMISNRNMIFMMRNL
                     QRMYGITSADEQLGFLPTAHVAAQMFYNFAVIESCSVINLVEELETTDRDQQEIAPTI
                     HFAVPRVWEKKHSLVTIKLKEGTVLGRWAYDLAMRIATPLAHARQRGETPSWISRLLY
                     RAVDPLVLKNIRVLLGIDRCRWLSTGAAPIAPELIDWYWALGRPMFEVYGQTECTGLA
                     TANHPGEVRIGSVGKPGQEIEVTLSEAGEILIRSPGVICGYWENPEKTAETFRQGWLH
                     TGDVGRIDEDGFVYVVDRMKDIIITAGGKNITPSEIENQLKFSPYITDAVVVGDRRPY
                     LTCLIMIDHENVTKYAQDHDVPFTNYTSLCHAREVQDLVEAEIEKVNARFARVETIKK
                     FRLIDQLLDEEDDELTPTMKLKRKVVHEKYGELIDTMY"
     gene            5280..6440
                     /locus_tag="AC16UZ_11480"
     CDS             5280..6440
                     /locus_tag="AC16UZ_11480"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6247347.1"
                     /translation="MENVYRNGLMAILSLLLLGACGGEATQTNEVVFGVHTDLSGPTA
                     IWGVGATNGARMRFDEANAAGGVHGRSIRYVVEDAGYQVPNAIRAANKLINRDQVLAM
                     VLAVGTPMNNAIMMEQFEQGVPNLFPISGARSMVEPLHPLKFTQRGIYYYEVRAAIRY
                     FVDSQGKQSVCAIYHDTDYGHEILEAVNDQLAEMNMALVESAAHKPSESEFTAAILRL
                     RNAGCDLVLMGTVHRDTILVLDSARKMGWEDVAWVGNNAAYGQVIADSPSCEGYYAFV
                     HMAKIYADDEKTPAVQAWWDHYVALYDEEPGLAAMEGYRAADLLVLALEKAGPDLSVG
                     SLLAALEGITDYTDLFGYNVSFGPEKHSGATESVLSQVQGGRWVTLAESISY"
     gene            complement(6452..8434)
                     /locus_tag="AC16UZ_11485"
     CDS             complement(6452..8434)
                     /locus_tag="AC16UZ_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HIR6247348.1"
                     /translation="MQSGDKAYAELDRPVFLTSASLLAAVIFLAALFPDTMDALFSDV
                     QGAIVAGASWYYVLVVAVILVSVLVFAFSSYGQIKLGPDHSEPDYSFISWFAMMFAAG
                     MGIGLMFFGVAEPVMHFLSPPKGDPGTIAAAREAMKLTFFHWGLHGWAIYAIVALILA
                     YFSFRHGLPLTLRSALYPLIGERIYGPIGTAVDVFAIISTTFGVATSLGLGVNQINAG
                     LNYLFGLPVTQEIRVGLIIVTTAIATVSVVLGLDRGIKRLSEINIVLAVMLMITVLAI
                     GPTTLLLQAFMQNTGDYLADLVGKTFNLYAYDPTDWLGGWTIFYWGWWISWAPFVGLF
                     IARISRGRTLREFVIGVLIGPVVFTFLWMTVFGNSAIDLIMNQGVTELGSAVQANESI
                     ALFRFLEQFPATGFLSALAIVMVVVFFVTSADSGALVLNMLSSNGSDQTPVGRRIFWM
                     SAIGVTAIVLLLVGGLSALQTAVIASALPFSVAVMAGIWGFSKALYVDHAKRVSHAAA
                     TVPPILARPEISWQHRLTQLLRHPQDAEVRAFMRERVMPVMREVGDELKRHGLDVEVI
                     VDGGEGESLRLEIAHGEEIDFVYEVVNRKHPLPDEALVGTAMKDMPPSNTYHRAEVHL
                     REGSQDYDIMGWTSEQIAHDITEQYEKHRHFLHMLR"
     gene            complement(8490..>9976)
                     /locus_tag="AC16UZ_11490"
     CDS             complement(8490..>9976)
                     /locus_tag="AC16UZ_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HIR6247349.1"
                     /translation="DYLRENPTHIPAMRLLAEIGVRLQVLDDAEFLLESALEFEPDHE
                     LARADYANVLIRRQKFTKALEALRPLLLQRPDHVPYRIAEAMATTGSGDHDAAITQYR
                     QIRASHPDLPQLHVSLGHAQKTIGDIDGAVASYQQAYGVRRDFGDAYWSLANTKTYRF
                     TPDEIDGMRQAEADPTCGTQDRIHLCFAAGKAFEDAGSYQESFAYYERGNRLKQGEIR
                     YDAGQIDRRVEAQMAVCTRELFARQEGHGDPAPDPIFVVGLPRAGSTLLEQILASHSM
                     VDGTMELPSLLAIAQRLRGRSSVRSDRYPRILGELKPEQLHQLGAHYLQETLPYRQGA
                     PRFVDKMPNNFFHVGLIRLLLPNARVIDARRHPMACCFSGFKQLFGEGQEFSYGLTEI
                     GHYYRRYVELMKHWDEVLPGFVLRVQHEDVVDDLEGQVRRILEFCELPFEDACVNFHR
                     TERAIRTPSSEQVRQPIYRDGLDQWQRFEAWLDPLKAALGDALG"
BASE COUNT         1781 a         3137 c         3246 g         1812 t
ORIGIN      
        1 gggtgcgtct atggattgat cgccctgggg ttcgtgctga tctacaaggc cacggagatg
       61 gtcaatttcg cccagggcga cgtcatgatg ctcggcgcgt tcctggcctt cgccctgatc
      121 aacctcatgg agcttccctt tctgtggggc ttcatgggcg cgctcgcggc catggccgtg
      181 gtgggggtgc tgctcgagcg gctgctgctg cgtccgatga tcggcgagcc gcacttcgcc
      241 gtgctcatgc tcaccatcgg gctcggtttc atcctgcgcg ccgtggccgg cgccatctgg
      301 ggcaacgagc ctcgtgccct gcacacgccg tatgggggag aagtgctgcg cttcggtgac
      361 ctggtggtcg gttacgagaa catcgtgatc atcgtcggca ccgccgtgct gtgcggcctg
      421 cttttcgcct tcttccgctt tacccgggtg ggtgtcgcca tgcaggccgc ttcccagaat
      481 cagctcgccg cttactacgt gggcattccg gtcaagcgca tctactccct tgtctgggct
      541 ctgtcggcga ccatcgccgc gtgcgcgggc gtactcatcg cgcccgtcac gctgattgac
      601 cctctgatgg ggagcttcct gggcatcaaa gcgttcgcgg cggcgatcgt tggcggtttc
      661 ggcagccttc ccggcgccat tctaggcggt cttctggtgg gcatcacgga gcagttcgtc
      721 gggctctatt ttccgccggg attcgccgat gtgagcgcct acgtgctgct gctcgtcatg
      781 ctcatgatcc ggcccgaagg gctcttcgcg accatgcagc gcaagaaggt ctgaggccgt
      841 gcgtttcatc cgcaagaccc actacgttca ggacgtcgac ctgatcaagc atccggggca
      901 gcgcttctgg tacgggctgc tcgccatcgt tctcgtcctc gcgcccctgg ggctcgaccg
      961 gttctacctg ggcgagctgt ctctcgtttt catctacgcc atcgccggca tcgggctgat
     1021 gctgctggtg gggtacacgg gcctcgtgag cctgggccat gcggcctttc tggccgtggg
     1081 cgcgtactgc cattcgtggc tgctgggcaa cggcgtgcct ctgcccctgg caatggttgc
     1141 ggcgaccctg gtcaccgccg gcgtcggcgc ggtggtcggc gtgcccgccc tgcggatgac
     1201 gggcatttac ctggccgtgg cgaccctcgc cctggccgtg atcgtcgaga aggtgctgga
     1261 gaaatgggag acggtgaccg gcggcctgcg tggcctgccc gtgcccaagg ctcagtttct
     1321 cggcttcgac ctcggcgctc cggccaactt ctactatctg tgtcttgggg tgcttgcgct
     1381 cggtctgctg gcggcgatga acctgctgcg cagccctacg ggccgggcca tggtcgccgt
     1441 gcgggactcc gaaacctcgg cccagagcat gggcattcac atcaaccgca tcaaggggct
     1501 ggccttcgcc ctgtcggccg gcttcacggg tcttgcgggc gcgctgtttg cgcaccggat
     1561 tggctacctc tttccggact cgttcaccgt gctcacctcc attcagctgc tgctgatggt
     1621 cgtcgtgggc ggcctcggct cgctgcacgg ggtcgtgttc ggggccatct tcatcggcct
     1681 gctgccccag gggatcgccc tgttcaggga cgtggtgccg ccggcgatcg cccagatacc
     1741 ggggctggag cccggcatat tcggcctcat tctcgtgctc ttcctgatat tcgaaccgct
     1801 gggtatatac ggccgctggg tcaagatccg ccttttcttc gaggagtttc cgctctatcg
     1861 gcgggcgacc tacaagcggc agaagaccta tctgcgcacg gagcgattgc ggtgagcctt
     1921 tttgccgcgg aggacgtcgc ggtgagcttc ggcggcatca aggccgtgca cgacctttcc
     1981 tttgccgtgg aagacggcga ggtgttcagc atcgtaggcc ccaacggcgc gggcaaaacg
     2041 acgatcttca acctcatcag ccgaatctac gacgtggacc aggggtccct tgccttcgac
     2101 ggcacagaga tcaccaggtt gccggcccac gccgttgccg agctcggcgt cgcgcgcacg
     2161 ttccagaaca ccgagctgtt cgagcaggaa acggtgctga aaaatctgct gatcggctgt
     2221 cacgtccatc gacgttccgg cctgcttgcc gacattctct ttctgccgtc cgtgaaggcc
     2281 caggagctga cttttcgcaa gcgggtggag gacgtcatcg atctgctgga tctgcagagc
     2341 taccgtgatc aggtgatcgg caatcttccc tatggcgttc gcaagatggt ggagatcgcc
     2401 agggcgctgt gcctgggccc gaagctgctg ctgctggacg agccttcgtc cggtctcaac
     2461 ccggaggaga ccgaggacct ctccttctgg ctggaggaca tcaacaacga tctcggcatc
     2521 accatcatca tggtggagca cgacatgaat ctcgtctccc gcgcttcgga ccgggtcatg
     2581 gcgctggcgg acggggcgct gctgaccatc gggtctccgg ccgacgtcca gcagcacccg
     2641 gacgtcctgc gggcgtacct gggggattga ccgtggacgc gatgctcgag ctcaagaacg
     2701 tggagaccta ttacggcccc atcatggcca tccgcggcgt cagcatggag gttcccgagg
     2761 gcaagatcgt ctccctgctc ggtgccaacg gcgccggcaa gactaccgtg ctcaagacca
     2821 tcagcggcgc catggatcct cagaagggca ccatcaccta ccagggcgag ccgatccagg
     2881 ctcgtgaccc ggactgggtg gcgcgccgcg gcatcgcgca cgtcccggaa ggccgcgagg
     2941 tcttcccctt tctctgcgtt gaggacaacc tgcgcatggg cgcgttctcc cgccgggacg
     3001 ccgagatcgg cgacgacatc gagctggtgt acggctattt tcccgttctc aaggagctcg
     3061 cgaggcagca ggccgagtac ctgtccggcg ggcagcagca gatgctcgcc atcggcaggg
     3121 cgctcatgct gcggccgcgg ctgatgctgc tcgatgagcc gtcactcggg ctgtcgcccc
     3181 ggcttgtcgc ggagatcgca gagatcatcg tccgcctcaa tcgcgagcag gccgtgacca
     3241 tgctgctggt cgagcagaac gcgaagatgg ccctggacat cggcgactac ggctacgtca
     3301 tggagatcgg ccgcatcgtc atggaagaca cctgtgagcg gctcaagaac gccacggaca
     3361 tcaaggagtt ctacctcggc atgaaagacg agggcgtgcg tggccggcgc cgctggaagc
     3421 agaggaagac ttggcgatga gcccgcagat catcgacggc tgcgcgacgg ttgcccggct
     3481 cttctggaag cgcgccacgg acctgcccga caagatcgcc ctgcgcgaga aggacttcgg
     3541 gatctggaac gagtacacct ggggcgactg gggcgagcag gcccggctgg tgggtctggg
     3601 gttgaaagcc ctgggcctga agcggggcga cgtctgctcc atcgcgaccg acgtcaacaa
     3661 ggagtggctt ttcgccgatc acggcgtgat cggcgtgggc ggcatcgtca acggcgtcta
     3721 ccccaccgat gcgccgaacc aggtcgagta tctgatcaac gacagccgga cgcgtttcta
     3781 ttttgccgag gacgaggagc agctcgacaa ggtgctcgag gtccgcggca acacgccgac
     3841 gctggagaag atcatcatct tcgacatgga ggggctgcgc acgttctccg acgagcagtg
     3901 catgagcttc gatgcgctgc tcgcgctcgg tcgcgagtac ggcgagcagc accccgggct
     3961 gtgggagcag gagatcgagg ctgccgaggc ggaggacctg ctcatcctga cctacacctc
     4021 cgggaccacg ggcaatccca agggcgcgat gatcagtaac cgcaacatga tcttcatgat
     4081 gcggaacctt cagcgcatgt acggcattac ctcggccgac gagcagctgg ggttcctgcc
     4141 cacggctcat gtggcggccc agatgttcta caacttcgcg gtgatcgagt cgtgctccgt
     4201 gatcaatctg gtcgaggagc tcgagaccac ggacagggat cagcaggaga tcgcgcctac
     4261 cattcatttc gcggtgccgc gcgtctggga gaagaagcac tcgctggtga cgatcaagct
     4321 caaggagggg accgtgctgg ggcggtgggc ctacgatctc gccatgcgca tcgcaacgcc
     4381 cctggcccat gcccgccagc gtggcgaaac cccgtcttgg atatcgcgcc tgttgtaccg
     4441 cgccgtcgat cccctggtgc tcaagaacat ccgcgtgctg ctcggcatcg accgctgccg
     4501 ttggctctcc accggcgcag cacccatcgc gccggagctg atcgactggt actgggcgct
     4561 gggaagaccc atgttcgagg tgtacgggca gaccgaatgc acgggtctgg ctaccgccaa
     4621 ccaccccggc gaggtgcgca tcggcagcgt cggcaagccc gggcaggaga tcgaggtgac
     4681 gttgtcggag gcgggcgaga tactgatccg cagcccgggc gtcatctgcg ggtactggga
     4741 aaaccccgaa aagaccgccg agacgttccg gcaggggtgg cttcacaccg gagacgtagg
     4801 gcgcatcgac gaggacgggt tcgtctacgt cgtcgaccgg atgaaggaca tcatcatcac
     4861 cgccggcggc aagaacatca cgccgagcga gatcgagaac cagctgaagt tctcgcccta
     4921 catcacagac gcggtggtgg tgggagatcg tcgtccttac ctgacctgcc tgatcatgat
     4981 cgatcacgag aacgtgacga agtatgctca ggaccatgac gtgccgttca ccaactatac
     5041 gagcctgtgc cacgcacgcg aggttcagga tctcgtggag gccgagatag agaaggtgaa
     5101 cgcgcggttc gcgcgggtgg agaccatcaa gaagttccgg ctcatcgacc agctgctcga
     5161 cgaggaagac gacgagctga cgccgacgat gaagctgaag cgcaaggtgg tgcacgagaa
     5221 gtatggcgag ctcatcgaca ccatgtactg acgcccgcac gggcacactg gagagctgaa
     5281 tggaaaacgt ctatcgcaac ggcctcatgg cgatcttaag tctgctcctg cttggggcgt
     5341 gcggtggaga ggcgacccaa acgaacgagg tcgtgttcgg cgtgcacacc gacctctccg
     5401 gccccacggc gatctgggga gtcggtgcca ccaacggcgc acggatgcgc ttcgacgagg
     5461 cgaacgccgc cgggggcgtg catggccgca gcatccgcta cgtggtggag gacgccggct
     5521 atcaggttcc caacgccata cgggccgcca acaagctcat caaccgtgac caggtgctgg
     5581 ccatggtgct cgcggttgga acgccgatga acaacgccat catgatggag cagttcgagc
     5641 agggcgtgcc gaacctcttt cccatcagcg gggcgcgctc gatggtggag ccgctgcacc
     5701 ctttgaagtt cacccagcgg ggcatctact actacgaggt gcgagcggcc attcgttact
     5761 tcgtcgacag ccagggcaag cagtcggtct gcgcgatcta tcacgacacc gactacgggc
     5821 acgagattct cgaggcggtc aacgaccagc tcgcagagat gaacatggcc ctcgtcgagt
     5881 ccgcggcgca caagccctcg gagagcgagt tcaccgccgc catcctgcgg ctgcgcaatg
     5941 ccggctgcga cctcgtcctg atgggaaccg tgcaccgcga caccattctc gtgctcgaca
     6001 gcgcgcgcaa gatgggctgg gaagacgtcg cctgggtcgg caacaacgca gcctacgggc
     6061 aggtcattgc cgactcgccg agctgcgaag gctattacgc gttcgtccac atggcgaaga
     6121 tttacgccga tgacgagaaa acgcccgccg tgcaggcgtg gtgggatcat tacgtggcgc
     6181 tgtacgacga ggaaccgggt ctggctgcca tggaaggcta tcgggcggca gatctgcttg
     6241 tgctcgccct ggagaaggcg ggtcccgatc tcagcgtagg gagcctcctt gccgcgttag
     6301 agggcatcac ggactacacg gacctgtttg gctacaacgt atctttcggt ccggagaagc
     6361 atagcggcgc cacggagtcc gtgctttctc aggtccaggg cggtcgctgg gtcacgctcg
     6421 ccgagtccat ttcctactag gctgccgccg atcagcgcag catgtgcagg aagtgccggt
     6481 gcttctcgta ctgctcggtg atgtcgtgag cgatctgctc gctcgtccag cccatgatgt
     6541 cgtagtcctg gcttccttct ctcaggtgca cctcggcccg gtgataagtg ttcgatggcg
     6601 gcatgtcctt catggcggtg ccgaccaggg cctcgtccgg aagcggatgc ttccggttca
     6661 ccacctcgta gacgaagtcg atttcctcgc cgtgggcaat ctccaggcgt aacgactcgc
     6721 cctcgccgcc atccacgatc acctccacat cgagcccgtg gcgcttgagc tcgtcgccga
     6781 cctcgcgcat cacgggcatg acccgctcgc gcatgaaggc cctcacttcg gcgtcctggg
     6841 ggtggcgcag cagctgtgtc agtcgatgct gccatgagat ctcgggcctg gcgagaatgg
     6901 gcggcacggt cgctgccgca tgggacacgc gtttggcgtg atcgacgtag agcgccttgc
     6961 tgaaccccca gattccggcc atgacggcca cggagaaggg cagggcgctt gcgatgacgg
     7021 cggtctgcag cgcggacaac ccgcccacca gcagcagcac gatggccgtc acccctatgg
     7081 cgctcatcca gaagatccgc cggcccaccg gtgtctgatc cgagccgttg gatgacagca
     7141 tgttcagaac cagggccccc gagtccgccg aggtcacgaa aaagaccacg accatcacga
     7201 tggccagggc agacagaaat ccggtggcag gaaactgctc caagaagcga aacagggcga
     7261 tggactcgtt ggcctgcacg gccgagccga gctccgtcac gccctggttc atgatcagat
     7321 cgatggcgct gttgccgaac acggtcatcc acaggaaggt gaagacgacg ggaccgatca
     7381 gcacgccgat cacgaactcc cgcagggtcc tgccccggga gatccgcgcg atgaagagcc
     7441 ccacgaaggg cgcccagctg atccaccagc cccagtagaa gatggtccat ccccccagcc
     7501 agtccgtggg gtcgtaggcg tagaggttga acgtctttcc caccaggtcg gccaggtagt
     7561 cgccggtgtt ctgcatgaac gcctggagga gcagggtggt cgggccgatg gccagcacgg
     7621 tgatcatcag catgacggcc aggacgatgt tgatctcgga cagccgcttg atgccccggt
     7681 ccaggccgag aaccacggag acggttgcaa tggccgtggt gacgatgatc agaccgacac
     7741 gtatctcctg ggtcaccggc agaccgaaaa ggtagttcag ccccgcgttg atctggttta
     7801 cgccgagccc gagggaggtg gccacgccga aggtggtgct gatgatggcg aagacgtcga
     7861 cggcggtccc gatgggcccg tagatgcgct cgccgatcag cgggtacagg gccgagcgca
     7921 gcgtgagcgg cagaccgtgc cggaagctga aatacgccag gatcagcgcc acgatcgcgt
     7981 agatggccca gccgtggaga ccccagtgga agaaggtgag cttcatcgcc tcccgggcgg
     8041 ctgcaatggt gcccgggtct cctttcgggg gcgagagaaa gtgcatcacc ggctcggcga
     8101 cgccgaagaa catcagcccg atgcccatgc cggcggcgaa catcatggca aaccaggaga
     8161 tgaaggaata atcgggctcc gagtgatcgg gcccgagctt tatctggcca tagctcgaga
     8221 acgcgaaaac cagcacgctc accagaatga cggcaaccac gagcacgtag taccagctcg
     8281 cgccggccac gatggcgccc tgcacatcgc tgaacagggc gtccatggtg tcgggaaaaa
     8341 gtgccgccag aaagatcacc gcggcgagca gcgacgcgga ggtcaggaat acgggtcggt
     8401 cgagctcggc gtaagctttg tcacccgatt gcattcacgt cccgcttgcg accaaagggg
     8461 ccccaccttc ttcgttccat ctcgaaaatt caaccaagcg catcgcccaa cgccgccttc
     8521 agcggatcca gccatgcctc gaagcgttgc cactgatcca ggccatcgcg gtaaatcggt
     8581 tggcgtacct gctcggagct cggcgtgcgg atggcacgtt cggtgcggtg gaagttcacg
     8641 caggcatctt cgaaaggtaa ctcgcagaat tccagtatgc gccggacctg gccctccagg
     8701 tcgtccacga cgtcctcatg ctgcaccctg agcacgaagc cgggcagcac ttcgtcccag
     8761 tgcttcatca gctcgacgta gcgccggtag tagtggccga tctccgtcag cccgtagctg
     8821 aattcctggc cctcgccgaa cagctgcttg aagccgctga agcagcaggc catgggatgc
     8881 cggcgcgcgt cgatgaccct ggcattcggc agcagcagcc ggatcagccc gacgtgaaaa
     8941 aagttgttgg gcatcttgtc gacgaagcgc ggggcgccct gccggtaggg cagggtttcc
     9001 tggaggtagt gagcgccgag ctggtggagc tgctctggtt tcagctcgcc gaggatccgc
     9061 ggatagcgat ccgagcggac cgagctgcgg cctcgcaggc gctgggcgat ggcgagcagc
     9121 gacggcagct ccatggtccc gtcgaccatg gagtgcgaag ccagaatctg ctcgagcagc
     9181 gtcgagcccg cccgcggcag cccgacgacg aagatcgggt ccggcgcggg atctccgtga
     9241 ccctcctggc gggcgaacag ctctctggtg cagaccgcca tctgcgcttc cacccgtcga
     9301 tcgatctggc cggcgtcgta gcgaatctcg ccctgcttca ggcggttgcc gcgctcgtag
     9361 tacgcgaagc tctcctgata gctgcccgcg tcctcgaagg ccttgcccgc ggcgaagcac
     9421 aggtgaatac ggtcctgcgt gccgcacgtc ggatccgcct ccgcctgccg catcccatcg
     9481 atctcgtcgg gcgtgaaccg ataggtcttc gtgttggcca ggctccagta agcatcgccg
     9541 aagtcgcggc gaaccccgta agcctgctgg tagcttgcga cggcgccatc aatgtcgccg
     9601 atggttttct gagcatgacc cagagaaacg tggagttgcg gcaggtcagg atggcttgca
     9661 cgtatctgcc gatactgagt gatggcagcg tcatgatccc cgctgccggt cgtggccatg
     9721 gcctcggcaa tgcgataggg cacgtgatcg ggccgctgga gaagcaaggg acgcagcgct
     9781 tccagcgcct tggtgaactt ctgcctgcga atcagaacgt ttgcgtaatc cgctcgagcc
     9841 agctcatggt cgggctcgaa ctccagcgcg ctttccagca gaaactcggc gtcatcgagc
     9901 acctgcaggc gcacgccgat ctccgccagc aaacgcatgg ccgggatgtg ggtcgggttt
     9961 tcccgcaggt agtccc
//
LOCUS       DBNHOX010000525         3792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1070882, whole genome shotgun sequence.
ACCESSION   DBNHOX010000525 DBNHOX010000000
VERSION     DBNHOX010000525.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1070882"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1728)
                     /locus_tag="AC16UZ_11495"
     CDS             complement(<1..1728)
                     /locus_tag="AC16UZ_11495"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6247350.1"
                     /translation="MTTYLRALNYPRTLLTLVALLLVVAGVCGTRFSFDASSDTLVVE
                     GDPDLATYLRIAETFGGDEFLLLTFTPRDGDALDPANLATLRALQQEIAALEGVGSVF
                     SILDAPLLKSPPVPLAELARGFRTLESADVDRPSAREELKESPVFGGLLITADARTTA
                     MRVDISLDRELLVLDEERARLRSQAERSAAEVKRLAELEVARETLREAYVNGRERLIG
                     QIRDVRDRYADRGVLHLGGVPMIAADMIAFVKSDLVVFGGSVVVIVMVTLYGFFRRLR
                     WVLLPVVTSAVTIGFTVGVLGLAQWPATVISSNFVSLLAIITISLTIHLVVRYRELAH
                     LHPDMAADELVRQTMVSKFAPCLYNALTTIAAFGSLTASQIVPVEDFGWMMCIGIAIG
                     FFTSFTLFPALLLLLGRVAGPSLLSRELGVTRVFSHLARWRAPLVVVISAVWAAFAAL
                     GVSMVSMDNRFLEYFRENTDINQGMAFVDAQLGGTVPFDVVLEFPPFENLVEEEDDFF
                     SEEETYPERYWFTRDKLDKLMLVHRYVEARPEVGKVLSLTSLEDLARQFTEGDALSNL
                     EVAAVLDVLP"
     gene            1800..2324
                     /locus_tag="AC16UZ_11500"
     CDS             1800..2324
                     /locus_tag="AC16UZ_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR6247351.1"
                     /translation="MVENIIQLIEEVAEALLENRSRLITAESCTGGMVAAELTSRPGS
                     SEWFEGALVTYRLSAKQRLLGVPAAMLDRYGPVSEPVARAMAEGALRVSDADVSVAIT
                     GIAGPDGGDMLAPVGTVWFAWARRDEPGAEPVCLQATQHRFSGSRDEVRTQAVALALE
                     GVLASLSGINPSRS"
     gene            complement(2305..2781)
                     /locus_tag="AC16UZ_11505"
     CDS             complement(2305..2781)
                     /locus_tag="AC16UZ_11505"
                     /inference="COORDINATES: protein motif:HMM:NF037143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6247352.1"
                     /translation="MDLNQIKADVGQTWKWLADGWRSLSDKAVNALTYFTPGRNEPAD
                     PDEIRWGLLAADVSESGEHVTVELEAPGLEKDEIDVSVDERQVTITGQKRVESERREG
                     AMVVRERAFGRFQRTIPMPVAVTADGAEASYRRGVLRIRVPKVASPGGRSIKIEKG"
     gene            complement(2791..3204)
                     /locus_tag="AC16UZ_11510"
     CDS             complement(2791..3204)
                     /locus_tag="AC16UZ_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6247353.1"
                     /translation="MSEPVRSGDPFDLFSRMMGGTDREPHAGSRDWLPAVDIREEQEG
                     YLLVMDVPGLGAEDLDVDLHEGVLTVRGARSAEETKQGEHYVRVERRHGKFARQFRVP
                     PSARPEDLRANVKDGVLTIHVPKGGAPETRRVPVS"
     gene            complement(3288..>3792)
                     /locus_tag="AC16UZ_11515"
     CDS             complement(3288..>3792)
                     /locus_tag="AC16UZ_11515"
                     /inference="COORDINATES: protein motif:HMM:NF039797.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HIR6247354.1"
                     /translation="KASMELTMNGMAKGLAAALTAGALLTGCASDPAETAAAQQEVSE
                     QIDTILTQPLGEEDYGGGERCISTNLYDSVDVLDTRHVLFKGRGNRLWVNTLRSTCVG
                     LRKRDVLQFEIRGSQICNLDTFTAVSNSFGWQRTSATCSLGDFSPVTAEQVAMIEAAL
                     DTKKKKK"
BASE COUNT          666 a         1275 c         1208 g          643 t
ORIGIN      
        1 gggcagcacg tccagaaccg cggcgacctc gaggttggaa agcgcatcgc cctcggtgaa
       61 ctgcctggcc agatcctcca gcgacgtcag cgagagaacc tttcccacct ccgggcgggc
      121 ctcgacgtac cggtggacga gcatcagctt gtccagcttg tcccgggtga accagtaccg
      181 ctcgggatag gtctcttcct ccgagaagaa gtcgtcctct tcctcgacca ggttttcgaa
      241 cggcgggaac tcgaggacca cgtcgaaggg caccgtgccg cccagctggg catcgacgaa
      301 ggccatcccc tggttgatgt ccgtgttctc tcgaaagtac tcgaggaagc ggttgtccat
      361 ggaaaccatg ctcaccccga gggcggcaaa cgccgcccac acggcggaga tgaccaccac
      421 cagcggtgcg cgccagcggg ccaggtggct gaagacacgg gtaaccccca gctcccgact
      481 cagcagggac ggccccgcga cccggccgag cagaagcagc agcgccggga agagggtgaa
      541 gctcgtgaag aaaccgatgg cgatgccgat gcacatcatc cagccgaaat cctccaccgg
      601 gacgatctgg ctggcggtca gcgatccgaa ggcggcaatg gtggtcaacg cgttgtacag
      661 gcacggcgca aacttgctga ccatggtttg ccggaccagc tcgtccgccg ccatgtcggg
      721 atgcaggtgg gcaagctcgc gatagcggac cacgagatga atggtcagcg agatggtgat
      781 gatcgccagc agggagacga agttggaaga gatgacggta gccggccatt gggccagtcc
      841 gaggacgccc acggtgaagc cgatggtgac ggcgctggtt acgaccggaa gcaggaccca
      901 gcgcagccgt cggaaaaacc cgtagagcgt gaccatcacg atcaccacga cgctgccgcc
      961 aaaaaccacg aggtcgctct tcacgaaagc gatcatgtct gccgcgatca tcggcacgcc
     1021 gcccaggtgg agaacgcctc gatccgcgta gcggtcgcgc acatcgcgta tctggccgat
     1081 cagacgttcc cggccgttga cgtaggcttc cctcaacgtc tcccgggcaa cctcgagctc
     1141 cgccagccgc ttgacctccg ccgcggacct ctcggcctgg cttcgcagcc gggcccgttc
     1201 ctcgtcgagc acgagaagct cccgatccag cgagatgtcc acccgcatgg ccgtggtgcg
     1261 agcatctgcc gtgatgagga gcccgccgaa caccgggctc tccttgagct cctccctggc
     1321 cgagggccgg tccacgtccg ccgactccag cgtgcgaaaa ccccgggcca gctcggccag
     1381 cggcacggga ggactcttca gcaacggcgc atcgagaatg gagaagaccg agccgacgcc
     1441 ctccagagcg gctatctcct gctgcaaggc gcgcagggtg gcgagattgg ccggatccag
     1501 cgcgtcaccg tccctgggcg tgaacgtgag cagaagaaac tcgtcgccgc cgaaagtctc
     1561 ggcgattctc aggtaggtgg cgaggtccgg atcgccctcc accaccagcg tgtccgagga
     1621 ggcatcgaag ctgaatcgcg tcccgcaaac gcccgcgaca accagcagca gcgccaccag
     1681 ggtgagcagc gtgcgcggat agttcagcgc ccgcaggtag gtcgtcacca gcggttgcag
     1741 gtgtttgtgc agatcggaga tacgcgtgct cgcggcaagg aattttcggc tattgttcgg
     1801 tggtggaaaa cattattcaa ctcatagagg aagtggccga ggcccttctc gagaaccgtt
     1861 cgcgcctgat caccgccgag tcctgcaccg ggggcatggt ggcagcggag ctcaccagcc
     1921 gacccggcag ctcggagtgg ttcgaggggg cgctggtgac gtatcgtctg agcgccaagc
     1981 agcgcctgct cggcgtgccg gcggcaatgc tcgaccgcta cggcccggtc agcgagcccg
     2041 tcgcccgggc gatggcagag ggcgcgctgc gcgtcagcga tgcggacgtg agcgtcgcga
     2101 taacgggcat tgccggtccc gacggtggcg acatgctggc cccagtgggc acggtttggt
     2161 ttgcctgggc caggcgcgac gagcccggcg ccgagcccgt ctgcctgcag gcgactcagc
     2221 atcgcttctc gggatctcga gacgaggttc gaactcaggc cgtcgccctg gccctggagg
     2281 gcgtgctcgc gagcctgagc gggatcaacc cttctcgatc ttgatgcttc ggccgcccgg
     2341 gcttgccacc ttgggcaccc ggatcctgag cacaccgcgc cggtagctcg cctcggcgcc
     2401 atcggcggtg actgccactg gcatggggat cgtccgctga aatcggccga acgcgcgctc
     2461 ccgaaccacc atggctcctt cacgccgctc ggattcgact ctcttctgac ccgtgatcgt
     2521 cacctgccgc tcgtccacgg acacgtcgat ctcgtccttc tcaagccccg gcgcttcgag
     2581 ctcgacggtc acgtgctcgc cgctttccga cacatcggcg gccaggagcc cccagcggat
     2641 ctcatcgggg tcggccggct cgttgcgccc cggcgtaaag tatgtgagcg cgttgacggc
     2701 cttgtcgctc aggctgcgcc aaccgtctgc cagccacttc caggtctgcc ccacatcggc
     2761 cttgatctga ttgaggtcca tggcgggctc ctagctgacg ggcacgcgcc gggtctccgg
     2821 tgcgccgcct ttgggaacgt gaatggtgag cacaccgtcc ttgacgttcg cgcgcagatc
     2881 ctccggcctc gcgctcggcg gaacgcggaa ctggcgggcg aacttaccat gacggcgctc
     2941 tacgcgaacg tagtgctcgc cctgcttggt ctcctcggcc gaccgggcgc cccgcaccgt
     3001 gaggacgccc tcgtgcaggt cgacgtccag gtcctcggcc ccaaggccgg gaacatccat
     3061 gaccagaaga tatccctcct gctcctcccg gatatccacg gccggcagcc aatcccggct
     3121 ccccgcgtga ggttcacggt ccgtcccgcc catcatccga ctgaacaggt cgaagggatc
     3181 tcccgatcgt acgggctcgc tcatggctgc tggctccaag ttggtctaga gccgtaatgg
     3241 tgcccccagc gggccgaatt tcactaagta cttcccgcag cagccggtca ctttttcttt
     3301 ttcttggtat ccagagcggc ttcgatcatg gcgacctgct cagcggtcac gggagagaag
     3361 tcacccagag agcaggtagc gctcgttcgc tgccatccga aggaattgct caccgccgtg
     3421 aacgtgtcga ggttgcagat ctggctgccg cggatttcga actgcagcac gtcgcgcttg
     3481 cgcaggccga cgcacgtgct gcgcagcgtg ttgacccaca ggcggttgcc gcgacccttg
     3541 aagagcacgt gccgggtatc caggacatct accgagtcgt agaggttcgt ggagatgcag
     3601 cgttctccac cgccatagtc ttcctcgccg agcggctggg tcaggatcgt gtcgatctgc
     3661 tcggaaacct cctgctgcgc cgccgccgtc tccgccggat cgctggcgca cccggtaaga
     3721 agcgcgccag cggtcagggc cgcggccaat cctttcgcca tgccgttcat cgtcagttcc
     3781 atgcttgcct tc
//
LOCUS       DBNHOX010000526         5355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_235420, whole genome shotgun sequence.
ACCESSION   DBNHOX010000526 DBNHOX010000000
VERSION     DBNHOX010000526.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_235420"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="AC16UZ_11520"
     CDS             complement(<1..1331)
                     /locus_tag="AC16UZ_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247355.1"
                     /translation="MLSRIFGNKDPLSQPDPAARRRAVEGLSEGKAGELQERLLELAR
                     SDADPGVRQACVSRLEGTSDLEQLLDDGLMANRAAERIAELLGPDASSELTRHPLVLR
                     ARLFRLAPEDAARLIDSIEDEDTLVDLTAGSREPLRGRILAGIRSAKALAALEQQSRG
                     RDKGLNRFAREGMERIKQLRREAQATLDRLNELADALERASRQAPGIAARQRYESLLR
                     EFDQAYERFAGHARALEGCNEPPRDASPIRARVAALVAPAEPVAISRQEETPASGGEA
                     FADLVPQLEALEGLMEAGHDFQTVSETRSRLTEAWLRAADHALPGDAEHRVFERVSHA
                     YQNLADAAGRLTAAGWQTHRDPLPSDLPDDPGEAAGFWQAAEERRRLLKRAEGLVRAV
                     AWPDWASPTAELRDLLDDVERLREEVARSDALGSSRHEDLKMLISTAASAI"
     gene            complement(1347..1586)
                     /locus_tag="AC16UZ_11525"
     CDS             complement(1347..1586)
                     /locus_tag="AC16UZ_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HIR6247356.1"
                     /translation="MSSPTIAQKAPYAVDVQAGRKYFWCACGRSAKQPFCDGSHEGTA
                     LTPLPYTAEKDATLYFCGCKHTKNVPLCDGSHNDL"
     gene            complement(1597..2454)
                     /locus_tag="AC16UZ_11530"
     CDS             complement(1597..2454)
                     /locus_tag="AC16UZ_11530"
                     /inference="COORDINATES: protein motif:HMM:NF006109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR6247357.1"
                     /translation="MAYNTILYDVEDGVLTLTLNRPERLNAFNHEMLSEMIDAFDRAD
                     ADDEVSSIIVTGAGRAFCAGADLGAGGDTFNADGREDRASGLHPDGGGRLTLRIYELK
                     KPIIAAVNGPAVGVGVTMTLAMDIRLAADVAKFGFVFARRGIVPEACSSYFLPRIVGI
                     SQALEWCYSGRVFPAEEALAGGLVRSLHSKEELMPAARALACEIADNTSAISVTLVRQ
                     MMWRMLGADHPMEAHKIDSRGVYFTGKSPDAREGVEAFLEKRPARFPGKVSSDLPEFF
                     PWWEERTFS"
     gene            2579..3295
                     /locus_tag="AC16UZ_11535"
     CDS             2579..3295
                     /locus_tag="AC16UZ_11535"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR6247358.1"
                     /translation="MPSTSSFDPLGSSSGLIVYVDFKSPYAYLAKDPTFQLADDLGID
                     LDWRPLTLDINSYLGSARLNASGAVAESRRTPEQWHRVRYAYRDARRYANLVGLTVRG
                     TTKIWDSSLASIGLQWAKGQGAGPLRAYIDLTYERFWKRELDIEDASVVAGVLAEAGA
                     DPDGFVDFARGEGRAEHDVMQQRIFAAGIFGVPSYVLDGELYFGREHLPRIRWLLTGR
                     PGPCPDVAYRHFDAQETIRG"
     gene            3288..3950
                     /locus_tag="AC16UZ_11540"
     CDS             3288..3950
                     /locus_tag="AC16UZ_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247359.1"
                     /translation="MAETEAYTFTAALTVCLDITQPESYLAVPGVRELAADLDMDVRW
                     LPYSVPPWPARSEPGPEADRGTWHRWHRARYRERDLRRYARLLSIDLGDLHRQIKGEL
                     AGLALLWVQDQPAAVGGALLDGLLEGHWSGALNIEEPASVTAMLVQAQARVAGWDEYR
                     SGRGPVELAALRSALSAAGVFDVPSLVVAHAVPEPEVFLGRAHLPMVRWLLTDRQGAP
                     PI"
     gene            complement(3952..4575)
                     /locus_tag="AC16UZ_11545"
     CDS             complement(3952..4575)
                     /locus_tag="AC16UZ_11545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6247360.1"
                     /translation="MQDDALPPQLFARADESPDSAFYREPRFVTHIDDATIDCLTAFY
                     RTFIPENAAVLDLMSSWVSHLPPECSYARVAGLGMNEAELAANPRLDDHVVHDLNAAP
                     ELPYPDSAFDRVILAVSIQYLTRPVEVLREALRTLREDGEICIAMSHRLFPTKAILAF
                     HRLGPADRVRLVSTYLELAGFLDVRFVDRSPDGADPLWLVVGRRAGR"
     gene            4768..>5355
                     /locus_tag="AC16UZ_11550"
     CDS             4768..>5355
                     /locus_tag="AC16UZ_11550"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6247361.1"
                     /translation="MDMRFTEDDLAFQAEVRSFIDRHWPADVRLARATVPAHGLDRPA
                     EEQAWFDALVARGWSVPNWPLEHGGTDWTPTQRFIWDRETVGAETPQMSVFGVTMLGP
                     ILCRWGSVDQQEKFLPPIREARVQWCQGYSEPGAGSDLASLKTTARLDGDHYTVDGEK
                     TWTSGAHVADWMFCLARTSSTNKPQEGISFLLIDMR"
BASE COUNT          835 a         1781 c         1796 g          943 t
ORIGIN      
        1 tcgatggcgc tcgccgcggt gctgatcagc atcttgaggt cctcgtgccg actgctgccg
       61 agggcgtctg accgcgccac ctcctcccgc agtcgttcca cgtcgtcgag caggtctcga
      121 agctcagccg tggggctcgc ccaatccggc caggcaacgg ctcgaacgag cccctccgcg
      181 cgcttgagca acctgcgccg ctcctccgcc gcctgccaga agccggctgc ttcccccgga
      241 tcatcgggca ggtcactggg caggggatca cgatgcgtct gccagccggc cgccgtcagc
      301 cgcccggcgg catcggccag gttctgatag gcgtgggaga cgcgctcgaa cacccgatgc
      361 tctgcatcgc ccggcagcgc gtggtcggcg gcgcgcagcc aggcttcggt cagacgggac
      421 cgggtctcgg ataccgtctg gaagtcgtgc cccgcttcca tcagcccctc cagcgcttcc
      481 agctgcggca cgaggtcggc aaacgcctcc ccaccgctcg ccggtgtttc ctcctgccgg
      541 ctgatcgcca ccggttccgc gggggcgacg agagcggcca cgcgggctcg gatgggtgac
      601 gcgtcccgag gcggttcgtt gcaaccctcc agcgcccggg cgtgcccggc aaaacgctcg
      661 taagcctgat cgaactcccg aagcagtgac tcgtagcgtt gtcgtgcggc gatgccgggc
      721 gcctgccgcg atgcgcgctc gagggcgtcg gcaagctcgt tcaggcggtc cagcgtggcc
      781 tgcgcctcgc gcctgagctg cttgatgcgc tccatgcctt cccgggcgaa gcgattgagc
      841 cccttgtccc gaccgcggct ctgctgctcc agagccgcca gcgccttggc cgatcggatt
      901 cccgccagaa tccttccgcg cagcggctca cgcgagccgg ccgtcagatc caccagcgtg
      961 tcctcgtcct ctatggaatc gatcaggcgg gctgcgtcct cgggcgccag gcggaacagg
     1021 cgggcgcgaa ggacgagcgg gtgtctggtc agctcgctgg aggcgtcggg gccgagcagc
     1081 tcggcgatcc gctcagctgc acggttcgcc atcagcccgt cgtcgagcag ctgctcgagg
     1141 tccgatgttc cctccagccg gctcacgcag gcctggcgaa cgccaggatc tgcgtcgctg
     1201 cgcgcaagct cgagcaaccg ctcctgcagc tcgcccgctt taccttccga cagcccctcg
     1261 accgcgcgcc gccgggccgc cggatccggc tggctcagag ggtccttgtt tccgaagatt
     1321 cgcgacagca tggcgatcct tcacgatcac aagtcgttgt gggagccgtc gcagagggga
     1381 acgttcttgg tgtgcttgca accgcagaag tagagcgtag cgtccttttc cgccgtgtac
     1441 ggcagcgggg tcagcgccgt gccctcgtgg gaaccgtcac agaagggttg cttggcgctg
     1501 cgtccgcagg cgcaccagaa atacttgcgg ccggcctgca cgtctaccgc gtagggtgct
     1561 ttctgggcaa tggtcggtga gctcatgtcg ttgtcctcat gaaaacgttc tctcctccca
     1621 ccaggggaag aattccggca gatcgctgct gaccttgccc gggaaccggg cgggccgttt
     1681 ttccaggaac gcttctacgc cctcccgagc gtccggagac ttgccggtga agtacacccc
     1741 acgggagtcg atcttgtggg cttccatggg atgatccgcg cccagcattc gccacatcat
     1801 ttggcgcacc agcgtcaccg agatcgcgga agtattgtcg gcgatctcgc aggcgagggc
     1861 tcttgctgcc ggcatcagct cctccttcga gtgcagactg cgcaccagcc cgccagccag
     1921 agcctcctcg gcggggaaca cgcggccgct gtagcaccat tccagtgcct ggctgatgcc
     1981 cacgatgcgt ggcaggaagt agctcgagca ggcttcgggg acgatgcccc ggcgtgcaaa
     2041 gacgaagccg aacttggcca cgtctgcggc caggcggatg tccatggcca gcgtcatggt
     2101 cacgcccacg ccgacggcgg gcccgttcac cgcggcgatg atcggcttct tgagctcgta
     2161 gatccgcagg gtcaggcggc cgccgccgtc cgggtgaagg ccgctggctc ggtcctcgcg
     2221 accgtccgca ttgaaggtgt cgccgccggc gccgaggtcc gcgccggcac agaatgcccg
     2281 accggcccct gtgacgatga tgctgctcac ctcgtcgtct gcatctgccc ggtcgaaggc
     2341 gtcgatcatc tcgctgagca tctcgtggtt gaacgcgttc agacgttccg gccgatttag
     2401 ggtcagcgtc agaacgccgt cttcgacgtc gtaaaggatc gtgttgtagg ccatgtgcgg
     2461 gggcgtcccc agatgagtag acagtggcgc atgttgccta gattcctgcg gattttccat
     2521 gcggactggc atgcgacctg gtttggcgcg ccgcgcaacc cctactacaa tcgcccgaat
     2581 gccgtccaca tcctctttcg acccgctcgg cagctcatcg gggctcatcg tctacgtcga
     2641 cttcaagagc ccttatgcct acctggccaa ggaccccaca ttccagctgg ccgacgatct
     2701 gggcatcgac ttggactggc ggccgctcac gctcgacatc aacagctact taggctctgc
     2761 gcggttgaac gcctcgggtg cggttgccga gagtcgccgt acgcccgagc aatggcaccg
     2821 ggtcaggtac gcctatcgcg atgcccggcg ttacgcgaac ctggtgggcc ttaccgtcag
     2881 gggcaccacg aagatctggg attcgtccct tgcatcaatt ggtctgcagt gggccaaagg
     2941 gcagggagcc ggcccgctgc gggcctatat cgatctgacc tacgagcggt tctggaagcg
     3001 cgagctcgac atcgaggacg catccgttgt tgccggcgtg ctcgcagagg cgggagcgga
     3061 ccccgacggg ttcgtcgact tcgcccgcgg cgaggggcgt gccgagcacg atgtcatgca
     3121 gcagcgtatc ttcgccgccg gcatcttcgg cgtgccgagc tatgtgctcg acggcgagct
     3181 ctacttcggc agggaacacc tgccgagaat acgctggttg cttacaggcc gaccgggccc
     3241 ctgtccggac gtggcctatc gtcacttcga tgcgcaagaa acgatccgtg gctgagaccg
     3301 aggcttacac gttcaccgcc gcgctcaccg tctgcctgga catcacccag ccggagtcct
     3361 atctggccgt tccgggcgtg cgcgagctcg ccgccgatct ggacatggac gtgagatggt
     3421 tgccctattc ggtgccgcct tggcctgcac ggtccgagcc gggccccgaa gcggatcggg
     3481 gaacctggca ccgctggcat cgtgcgcgat atcgggagcg ggacctgcgc cgctacgccc
     3541 gtcttctcag catcgatctg ggggacctgc accggcagat taagggcgag cttgccgggc
     3601 tcgcgctgct ctgggtgcag gatcagccgg ccgccgtggg cggcgctttg ctcgacggcc
     3661 tgctggaggg tcactggagc ggggcgctga acattgagga gccggcatcg gtgaccgcga
     3721 tgctcgtcca ggctcaggcc cgcgttgccg gctgggacga gtatcgcagc gggcgcggcc
     3781 ccgtcgagct ggcagccctg cgctccgcgc tcagcgcagc gggggttttc gatgtccctt
     3841 cgctcgtcgt ggcccacgcg gtgcccgagc ccgaagtctt cctcggtcgc gcgcatctgc
     3901 cgatggtccg ctggttgctc acggatcggc agggcgcgcc gcccatctga ttcagcgccc
     3961 ggcgcgacgt ccgacgacca gccacagggg atctgcgccg tcgggggatc ggtcgacgaa
     4021 gcgcacgtcc agaaaaccgg cgagctccag ataggtggat accagccgca cgcggtcggc
     4081 ggggccgagc ctgtggaagg cgaggatcgc cttggttgga aacaggcggt gggacatggc
     4141 gatgcagatc tcgccatcct ctctgagggt gcgcagggct tcgcgcagta cctccacagg
     4201 gcgtgtcagg tactggatcg aaaccgccag gatgacgcgg tcgaacgcgc tgtccgggta
     4261 cggcagctcc ggtgcggcgt tgagatcgtg caccacatgg tcgtcgagcc tcgggttggc
     4321 tgccagctcc gcctcgttca tgccgagacc ggcaacgcgc gcgtagctgc actccggcgg
     4381 caggtgggat acccaggacg acatcaggtc caggaccgcg gcgttctcgg ggatgaacgt
     4441 gcggtagaag gccgtgaggc agtcgatggt ggcatcgtcg atgtgcgtca cgaagcgcgg
     4501 ttcgcgatag aaggccgaat ccggtgactc gtcggcccgg gcgaaaagct gcgggggaag
     4561 ggcgtcgtcc tgcaaggtgg atccgaaccg ttgctgcttg ggccgtcatt ctagcgaatg
     4621 cggccaatgg catgttgccc gcggcctgcc ctgtcctcgg aggcgagccc ggcctccgtt
     4681 acccgatgat ccgggcccca agaccgcgca gataggagct gataagattc cgcggtttcg
     4741 catctcgggg actttcgcgg gaccaccatg gacatgagat tcaccgaaga cgaccttgcc
     4801 tttcaggccg aggtgcgttc gttcatcgat cggcactggc ctgccgacgt tcgcctggcc
     4861 cgggccaccg tgcccgccca cggcctcgat cgccccgccg aggagcaggc ctggttcgac
     4921 gcgctcgtgg cgcgcggttg gagcgttccg aactggccct tggagcacgg cggtaccgac
     4981 tggacgccca cccagcggtt catctgggat cgcgaaaccg tgggggcgga aacgccccag
     5041 atgagcgtgt tcggcgtaac gatgctggga cccatcctgt gccgttgggg aagcgtcgat
     5101 cagcaggaaa agtttctccc gcccattcgg gaagcccgcg tccaatggtg tcagggctac
     5161 tcggagcccg gagcgggatc ggacctggca agcctcaaga cgacggcgcg gctggacggc
     5221 gatcactaca cggtcgatgg ggagaaaacc tggacgagcg gcgcgcacgt ggcggactgg
     5281 atgttctgcc ttgctcggac ctcgtcgacg aacaagcccc aggagggaat cagcttcctg
     5341 ctcatcgaca tgcgc
//
LOCUS       DBNHOX010000527         3565 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1455703, whole genome shotgun sequence.
ACCESSION   DBNHOX010000527 DBNHOX010000000
VERSION     DBNHOX010000527.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1455703"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1500
                     /locus_tag="AC16UZ_11555"
     CDS             <1..1500
                     /locus_tag="AC16UZ_11555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6247362.1"
                     /translation="PKQHEYLSSIRAASENLLSLVNDVLDFSKIEAGKLTLEHRPFKL
                     ADTLKEVERLFRTEVRKSNLELNFDDNTAAHAEFPGDGVIIGDPLRLQQVLVNLVSNA
                     LKFTEEGSISVTAQVCPCEAGELRVEFAVRDTGIGIKPEHQARLFESFEQAETSTTRR
                     YGGTGLGLTICKRLVSLMGGEMSVASVPDQGSCFTFTAVFGLRDAQDLMPADVRQRRR
                     NLRALNRREFLVAEDNPINQQLSLEFLQRTGARVDIAQTGREAVERATSKAYDAILMD
                     IHMPELDGLQACRILREQQITTPIIAVSADAIEASQQAALEAGCNAYVSKPIDFDLLL
                     AELSRVLPEQPADAASVDAPASAPAEHEASTTPGELPEIQLPGIDLDTAIRGHNGNVR
                     LMLKLMGDFGRYYGDAGAKMRAHVTDNHLEEAERLAHNLHGVAGSFGADRLKQASKTL
                     ELALAKGDESNLLGLVQSFEVAMNEVLESAETLANHPFTEAASRSRNEG"
     gene            complement(1463..2314)
                     /gene="ttcA"
                     /locus_tag="AC16UZ_11560"
     CDS             complement(1463..2314)
                     /gene="ttcA"
                     /locus_tag="AC16UZ_11560"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HIR6247363.1"
                     /translation="MRKHGYDMNKLHRKLRRKVGQAIADYGMIEEGDRVMVCLSGGKD
                     SYTLLDILLNLQRSAPVAFEIVAVNLDQKQPGYPADVLPGYLEGLDVSYHILEQDTYS
                     IVKAVTPAGKTTCPVCSRLRRGILYNFAASIGASKIALGHHLDDVVETLFLNMFHGAR
                     LKAMPPKLKSDDGRNVVIRPLYYCRERDIARWAEHREYPIIPCNLCGSQENLQRQVIK
                     SMLARWDAEQPGRVENIARSLSAVVPSHLGDRGLFDFAGLSSDDGVEKLQLLDLTPRC
                     VNAMPPR"
     gene            complement(2428..>3565)
                     /locus_tag="AC16UZ_11565"
     CDS             complement(2428..>3565)
                     /locus_tag="AC16UZ_11565"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6247364.1"
                     /translation="LALAGLQRRRRENVAQILIFGLAIMLLLILVLLRSSLLDEWQGQ
                     VPEDAPNHFVLNIVPHEVDELATMLDDFTGSHGGLFPMIRGRIVAANGVDADAWERDH
                     QPVDAPGPRLQSERNLTWAADLPDDNQVVKGRWWSPDEARALVSLEDEYAQSLGLDVG
                     DRLRFDIAGRFVEVEVASLRRLDWESMRPNFFIIFAPQVLSDFPATYMTSFHLERDEK
                     PFLNRFLARFPTVTVIEVDAIIAQVQSIILRVTQAVELVLALVVASGCLVLVASIQAS
                     RDGRMSELALVRTLGGSRRLIAGSLVAEFAALGLFAGTVAVVGAEATVAILQSQVFEL
                     PVRLHPWLWLLGPVIGSVLILIVGTLGTRGLISTPPVMVLRGLD"
BASE COUNT          694 a         1161 c         1142 g          568 t
ORIGIN      
        1 cccaagcagc acgagtacct gtcctccata cgggccgcat cggagaacct gctctcgctg
       61 gtcaacgacg ttctggactt ctcgaaaata gaagcgggca agctcaccct cgaacaccga
      121 cccttcaagc tggccgacac gctcaaagag gtggaacgtc tgttccggac cgaggttcgc
      181 aaaagcaatc tcgagctcaa cttcgacgac aacaccgcgg cgcacgccga gtttcccggc
      241 gacggcgtga tcatcggcga ccccctgagg cttcagcagg tgctggtcaa tctggtcagc
      301 aacgcgctga agttcaccga ggaggggagc atctcggtca cggcccaggt ctgtccctgc
      361 gaggccggcg agctgcgcgt cgagttcgcc gtcagagaca ccgggatcgg gatcaaaccc
      421 gagcaccagg cacgactctt cgagtcgttc gagcaggcgg agacctccac cacccggcgc
      481 tacgggggca ctgggctggg gctgacgatc tgcaaacggc tggtgtcgct gatggggggc
      541 gagatgtccg tggcctcggt gcccgaccag ggaagctgct tcaccttcac cgccgtgttc
      601 ggccttcgcg acgctcagga cctgatgccc gcagacgtgc gtcagcggcg gcgcaacctg
      661 cgcgcgctaa atcgcagaga gttcctggtc gcggaggaca accccatcaa ccagcaactg
      721 tccctggagt tcctgcagcg caccggggca cgggtggaca ttgcccagac cgggcgcgag
      781 gcggtggagc gcgcgaccag caaagcatac gacgcgattc tcatggacat acacatgccc
      841 gaactggatg ggctccaggc atgccggatc ctgcgcgagc agcagatcac gacgcccatc
      901 atcgcggtct ccgcggacgc catcgaagcc agccagcaag cggcgctgga ggcgggctgc
      961 aacgcttacg tgtcgaagcc catcgacttc gacctgctgc tggccgagct gtcccgggtg
     1021 ctgcccgagc agccggcgga cgccgctagc gtcgatgctc cggcgtccgc gcctgccgag
     1081 cacgaagcgt caacgacgcc gggcgagctt cctgagattc agctgcctgg aatcgacctg
     1141 gacacggcca tcaggggaca caacggcaac gtccggctaa tgctgaaact catgggggat
     1201 ttcggcaggt actacggcga cgccggggca aagatgcgtg cccacgtgac cgacaaccat
     1261 ctcgaggagg ccgagcggct cgcccacaac ctgcacggcg tggccggcag cttcggggcc
     1321 gaccgcctca agcaggcttc aaagaccctc gagctcgctc tggccaaggg cgacgagagc
     1381 aacctgctcg ggctggttca gagtttcgag gtggccatga acgaggtgct ggaatcggca
     1441 gagaccctgg ccaaccaccc cttcaccgag gcggcatcgc gttcacgcaa cgaggggtga
     1501 gatcgagcag ctgaagcttc tcgacgccat cgtccgagga aaggccggcg aaatcgaaaa
     1561 ggccgcggtc gccgagatgg gacggcacca cggcactgag gctgcgggca atattctcca
     1621 cgcgtccggg ctgctcggcg tcccagcggg ccagcatcga cttgatcacc tgccgctgaa
     1681 gattctcctg ggaaccgcag aggttgcagg ggatgatggg atactctcgg tgctcggccc
     1741 agcgggcaat gtcgcgctcc cggcagtagt agagcggtcg tatcaccacg ttgcgtccgt
     1801 cgtcgctttt cagcttcggc ggcatggcct tcagccgcgc cccatggaac atgttgagaa
     1861 acagcgtttc caccacgtca tcaaggtggt gtcccagcgc gatcttgctc gcgccgatgc
     1921 tcgcagcgaa gttgtagaga atgccgcgcc gcagccgcga gcaaaccggg caggtcgtct
     1981 tgcccgccgg agtgaccgcc ttgacgatgg aatacgtgtc ctgttccagg atgtgatagg
     2041 aaacgtccag cccttccagg tacccgggca gcacgtccgc cggatacccg ggctgcttct
     2101 gatccaggtt aacggcgacg atctcgaagg cgaccggcgc gcttctctgg agattgagca
     2161 gaatgtccag cagcgtgtag gaatccttgc ctcccgacag gcagaccatc acccgatcgc
     2221 cttcctcgat cattccgtaa tcggcgatgg cctggccaac tttgcgcctg agctttctgt
     2281 gcagcttgtt catgtcgtag ccgtgcttgc gcattgcgcg ctccgacctc gcggcggggt
     2341 ccattgggtc tgccttcatc agggccttgg gatggcgtgg gaacggattt ttagcagcac
     2401 ggatcggcgc tgacaactca cgacgggtca atccagaccg cgcagcacca tgacgggggg
     2461 cgtagagatc agcccgcgcg tgcccagggt cccgacgatg aggatgagca cggacccgat
     2521 caccggaccc aggagccaca gccagggatg cagacgcacc ggcagctcga aaacctggct
     2581 ttgcaggatg gccaccgtgg cttccgcccc gaccacggcg accgtgccgg caaacaggcc
     2641 gagcgcggcg aattcggcga ccagcgaccc cgcgatgagg cggcggctgc cccccagcgt
     2701 gcggaccagc gcgagctcac tcatgcggcc gtcgcggctg gcctggatgc tggccaccag
     2761 caccaggcat ccggacgcca ccaccagggc gagcacgagc tcaacggcct gggtcactct
     2821 gaggatgatg ctctgaacct gagcaatgat ggcatcgact tcgatgacgg tgaccgtggg
     2881 aaacctggcc aggaaccggt tgagaaacgg cttctcgtcc cgctcgagat gaaagctggt
     2941 catataggta gcggggaaat cgctgagcac ctggggagcg aagatgatga agaaattggg
     3001 gcgcatgctc tcccaatcga gccgccgcag gctggcgact tccacctcca cgaagcgacc
     3061 ggcaatgtcg aagcgcagcc ggtcgccgac gtccagccca aggctctggg catactcgtc
     3121 ctcgagggaa accagggccc gggcctcgtc cggcgaccac cagcgtcctt tgaccacctg
     3181 attgtcgtcc ggcaggtcgg cggcccaggt gagatttcgc tccgactgaa gtcgcgggcc
     3241 cggcgcgtcc accggttgat ggtcccgctc ccacgcgtcg gcatccaccc cgttggcggc
     3301 cacgatgcgg ccgcggatca tgggaaacag gcccccgtgg ctgcccgtaa aatcgtccag
     3361 catggtcgcg agctcgtcca cctcgtgcgg aacgatgttg agaacgaaat ggttgggcgc
     3421 gtcttccggc acctgtccct gccactcgtc gagcaacgac gagcgaagca ggaccaggat
     3481 cagcagcaac atgatggcga gaccgaaaat cagtatctgg gccacgttct cccgacggcg
     3541 acgctgcaga ccggccagcg ccagc
//
LOCUS       DBNHOX010000528         1720 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_428148, whole genome shotgun sequence.
ACCESSION   DBNHOX010000528 DBNHOX010000000
VERSION     DBNHOX010000528.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_428148"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="AC16UZ_11570"
     CDS             complement(<1..548)
                     /locus_tag="AC16UZ_11570"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR6247365.1"
                     /translation="MLRIAHDALTFDDVLLLPAYSQCLPSEVSLATKLTRDIHLNIPL
                     MSAAMDTVTEARLAIAIAQEGGVGIVHKNMDIESQAREVRAVKKHESGIIRDPITIHP
                     DKTVADLIELTMEHKISGVPVVDGRRLVGIVTGRDVRFERRMDAKVRDVMTTKEHLVT
                     ADEEASFEKITDLLHKHRIEKVL"
     gene            607..>1720
                     /gene="xseA"
                     /locus_tag="AC16UZ_11575"
     CDS             607..>1720
                     /gene="xseA"
                     /locus_tag="AC16UZ_11575"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR6247366.1"
                     /translation="MLASQADRPVYSVSELAHEARILIEERFDLVWVEGEISNFRRPA
                     SGHWYFTLKDRSAQVRCAMFAGRNRQVRFAPEDGMQVLIRGRVSLYEPRGDFQVIVEH
                     AEPAGEGALRQAFEALKTKLAVEGLFAIERKRPLPALPRHLAIVSSASGAALQDVLHV
                     IERRFPALTVTLLPVSVQGNEAEVQIRRALARVDQVGADVVLITRGGGSLEDLWAFNL
                     ESVARAVAACPIPTVVAVGHQTDFVIAEFVADLRAPTPSAAAELITPDAAELAGRVGL
                     LQQRLVRGLSADVRLRQALVGRLRAQLVDPRQMVTRSMQRADDLEERLRLALGNRLER
                     NRNRLWALQRSLRAFRPEPLLRQQAARVTATRHALGK"
BASE COUNT          274 a          580 c          560 g          306 t
ORIGIN      
        1 aggactttct cgatgcgatg cttgtgaagg agatcggtga tcttctcgaa gcttgcctcc
       61 tcgtccgcgg tgacgaggtg ctcctttgtg gtcatcacgt cgcggacctt ggcgtccatg
      121 cgacgctcga accgcacgtc gcgtcccgtg acgatgccga ccagccggcg tccatccacc
      181 accggcacgc cggagatctt gtgttccatg gtcagctcga tcagatccgc gaccgttttg
      241 tccggatgga tggtgatcgg gtcacggatg atgccacttt cgtgcttctt gaccgcccgg
      301 acttcacggg cctggctctc gatgtccatg ttcttgtgca cgatgccgac cccgccctcc
      361 tgcgcgatcg caatagcaag ccgagcctcc gtcaccgtgt ccatggcagc ggacatgagc
      421 gggatattga ggtggatgtc tctggtcagc ttcgtcgcga gcgaaacttc tgaaggcaag
      481 cactgggagt aggcgggaag gagcagaacg tcgtcgaacg tgagcgcgtc atgcgcaata
      541 cgcaacattg gtcactccgc aaaagcggca ttctatcggc gcaccccgat catgtaaact
      601 cgacccatgc tcgccagcca agcagatcgc cccgtctaca gcgtcagcga actcgcccac
      661 gaggcgagaa tcctcatcga ggagcgcttc gacctggtct gggtggaggg cgagatctcc
      721 aacttccggc gtcccgcatc cggccactgg tactttacgc tcaaggatcg ctccgcccag
      781 gttcgctgcg cgatgttcgc cggccgcaac cgccaggtac gcttcgctcc cgaggacggc
      841 atgcaggtcc tgatcagggg ccgtgtcagc ctttacgagc cgcgcggcga cttccaggtc
      901 atcgtcgagc acgcggagcc cgccggcgaa ggcgcgctgc gccaggcctt cgaggccctg
      961 aagaccaagc tcgcagtgga aggtctcttc gcgattgagc gtaagcggcc gctgcccgcg
     1021 ctgccccgcc acctggcaat cgtctcgtcg gcctccggcg cggctctcca ggacgtcctc
     1081 cacgtcatcg agcggcgctt tccggcgctg accgtcacgc tgctgccggt cagcgtgcag
     1141 gggaacgagg ccgaggtcca gatccgacgc gcgctggcgc gggtcgatca ggtcggcgcc
     1201 gacgtggtgc tgatcacgcg cggcggaggg tctttggagg atctctgggc gttcaacctc
     1261 gaaagcgtcg cccgcgcggt ggcagcgtgc ccgataccca cggtggtggc cgtgggccac
     1321 cagaccgact tcgtcatcgc cgagtttgtg gccgacctgc gcgcacccac gccatccgcc
     1381 gccgccgagc tcatcacgcc ggatgccgca gagctggccg ggcgcgttgg gctgcttcaa
     1441 cagcgactgg tccgcgggct ttccgccgac gtgcgcttgc ggcaggcgct ggtggggcgg
     1501 ctgcgagccc agctcgtcga tccgcgccag atggtgaccc gcagcatgca gcgtgcggac
     1561 gatctggagg agcgcctgcg gctggccctc ggcaaccggc tggagcggaa ccgaaaccgg
     1621 ctgtgggcat tgcagcgcag cctccgggcc ttccgtccgg agcccctgtt gcgccagcaa
     1681 gcggccaggg tcaccgccac gcgtcacgcc ctgggaaaat
//
LOCUS       DBNHOX010000529         6980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1777760, whole genome shotgun sequence.
ACCESSION   DBNHOX010000529 DBNHOX010000000
VERSION     DBNHOX010000529.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6980
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1777760"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /gene="dut"
                     /locus_tag="AC16UZ_11580"
     CDS             <1..321
                     /gene="dut"
                     /locus_tag="AC16UZ_11580"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR6247367.1"
                     /translation="VYGTGLAFEIPQDHVMLVFSRSGHGFKEAVRLSNCVGVIDSDYR
                     GEVKVSLRTDADDYKLPAEAGERIAQAMILPVQQVSFLEVDELGTTERGTGGLGSTGS
                     GALV"
     gene            417..824
                     /locus_tag="AC16UZ_11585"
     CDS             417..824
                     /locus_tag="AC16UZ_11585"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HIR6247368.1"
                     /translation="MGARTLAGDPAGTVHHKRSLQVCIAGRIYQSHRIAWLLAYREWP
                     QGCVDHIDGNSLNNRLDNLRDVSHRTNCENKHRPSRSNTSGYLGVSRFRDKWQAQIKV
                     KGKVIPLGRFEDPVVAHEAYLAAKRSLHIGCTI"
     gene            865..2295
                     /locus_tag="AC16UZ_11590"
     CDS             865..2295
                     /locus_tag="AC16UZ_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012403.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HIR6247369.1"
                     /translation="MLFTPRPYQMLIAGHILDHPRCNVFASMGSGKSASTIYAFDLLK
                     MLGEARRLLVLAPLGVARTTWPVEVQKWSESFGHLSVAAAVGTPDQRLAALRANADIT
                     TTNYESIEWLIETVGDHWPWDMVVADESTLLKGLRVSLQTSSKGTEFIAGQGSVRAKA
                     LSRVALRHVRRWVCLTGTPAPNGLQDLWGPTFFVDGGQRLGRSFTAFQNRWFVPEGRS
                     SNPAAQKYKPYPWSEKQIQDAIKDVSITIDAKDWFDIKDPLESLVLVDLPPAARKHYN
                     EVEKELFTELASGAEVEVFNPAGRSNKCLQIASGFVIHDDQGSWEKLHDAKIDALKTE
                     ISAAGGEPVLVAYQFIAERERILKAFPRAKLIGDDPQTEADWNKGKIPVLLAHPKSAG
                     HGRNLQHGGRTLIDFSSGWNLEYDQQILERIGPTRQIQSGYDRTVFRRRIVARDTVEE
                     LAVIPRLKSKADVQTALLNAMKQRTA"
     gene            2359..2868
                     /locus_tag="AC16UZ_11595"
     CDS             2359..2868
                     /locus_tag="AC16UZ_11595"
                     /inference="COORDINATES: protein motif:HMM:NF023181.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HIR6247370.1"
                     /translation="MSTTNSKWLPPQQEGCGPWIEFTGPHGERPVPGDTLVGYLLADE
                     REAQQYNHSVRRADRYSWGDNICAAYCVKLDGLQPQQPAASDRPAATPEQAPDTALAV
                     QVAGDHYKRLAIQPIEYIHANGIGFAEGSVIKYVTRWRDKAGITDLKKARHFLDLLIE
                     LEEKKAGAA"
     gene            2865..3533
                     /locus_tag="AC16UZ_11600"
     CDS             2865..3533
                     /locus_tag="AC16UZ_11600"
                     /inference="COORDINATES: protein motif:HMM:NF019225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HIR6247371.1"
                     /translation="MIDLHNPSGCLHVGRRRKTRSSTQRRIVMTLAYTTLAAVLAATA
                     FSASAQHAATITVVGTDIAQVTFRNDGPTALVAAEFFLGTPNRGRAIFSNVGSPTGAA
                     DFAFGDGVHYSVTTFDFGALQQGASITETVNFDQYDAAGEGSPFSTDATGTFAYMASR
                     MTLVWADGFEKIVPTSRAPIDAVFSFTDIPPVPPVSEPGTAALTLAGLGALAVALRKK
                     GGAA"
     gene            3530..3817
                     /locus_tag="AC16UZ_11605"
     CDS             3530..3817
                     /locus_tag="AC16UZ_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247372.1"
                     /translation="MKIDDISLAIAEAERFIERACVLRSTLVADETRVEAELAANPAA
                     YAYHHSFPKEQGSVRRASMDLTRALAKMRRPGRSTPPPNCARWRRPHSPRS"
     gene            3929..4126
                     /locus_tag="AC16UZ_11610"
     CDS             3929..4126
                     /locus_tag="AC16UZ_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247373.1"
                     /translation="MADNIERKPTAEEILDYLDEDKGKTVVHLGARWYTRPNYGQPLR
                     RRLSLRDAASVAMSWKETKNG"
     gene            4119..4331
                     /locus_tag="AC16UZ_11615"
     CDS             4119..4331
                     /locus_tag="AC16UZ_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247374.1"
                     /translation="MADDLDLAAEREEIARTAALSQRRPAGPQATGFCLWCEEPVAPL
                     VRFCCPECRDQFEAATTRKRQLGARN"
     gene            4345..4845
                     /locus_tag="AC16UZ_11620"
     CDS             4345..4845
                     /locus_tag="AC16UZ_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247375.1"
                     /translation="MSTTNSKWLPPQLAGFGPWIERTRGSGAPVLSGEVRICTSWERQ
                     IREYVPSTGDAAGVNWDGETAASAVVAYCVKLEEQPVEPQQPAASGLTRLVPLRQKAL
                     HTLEETTISDPQVAFWGVSITGVPMVRLATLADAQLVQRLADAAFQAGRQKAFSDLAD
                     WVSARP"
     gene            5110..5466
                     /locus_tag="AC16UZ_11625"
     CDS             5110..5466
                     /locus_tag="AC16UZ_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247376.1"
                     /translation="MTSLYTILDHLRGEPVPDDWTEIKNPQTGAVVGWIGDDLKEALQ
                     NHPAFTGEEFRMIVSGVRLSPEDVSVDPVAGTITVRSGALPADSHCDIYYAKPPTKKH
                     KQAQWKRETAAFGRRK"
     gene            5474..5755
                     /locus_tag="AC16UZ_11630"
     CDS             5474..5755
                     /locus_tag="AC16UZ_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247377.1"
                     /translation="MSKIQRRNNQGGVTHVADLLPTGRALAPREPVRCDLPPVNPATG
                     RPYFTLQREPMGSGWNGAQNPVDAAKLAFDRKMADALRHDSDSEGGHAD"
     gene            5745..6065
                     /locus_tag="AC16UZ_11635"
     CDS             5745..6065
                     /locus_tag="AC16UZ_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247378.1"
                     /translation="MPTDLKPFLQTCRDVAASLKPNGEDYDNLLYFGECLEDAWNAIL
                     PVVCRGLLLVTCPISVPVCAYLLRKWAREDEARRTARRALAAAAARGVGSFQEGGRHE
                     RQAD"
     gene            6290..>6980
                     /locus_tag="AC16UZ_11640"
     CDS             6290..>6980
                     /locus_tag="AC16UZ_11640"
                     /inference="COORDINATES: protein motif:HMM:NF037695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247379.1"
                     /translation="MSNTPSARAQIITRRTYNRPLDEDGTVFETWAQTVDRVIRHQKW
                     LWERAQKRAINFAQGEELEELRELLMERKGLMSGRTLWLGGTDIAKTRMASQLNCTFR
                     RVATVHDAVDVFWLLLQGAGTGFDGTPGTLNGFSRKLDVQIIRSTRTLEQWAAGERGD
                     PMNHEFVDGDTWTIRIGDSAAAWAKAVGKLLAGKAVVKTLVLDLREIRASGVRLKGYG
                     WVSSGDGPLSKA"
BASE COUNT         1395 a         2361 c         2180 g         1044 t
ORIGIN      
        1 gtctacggca cggggctcgc gttcgagatc ccccaagacc acgtcatgct ggtgttcagc
       61 cgcagcggcc acgggttcaa ggaggctgtc cggctctcga actgcgtggg cgtgatcgac
      121 tcggactacc gcggcgaggt caaggtgtcc ctccggacgg acgcggacga ctacaaactg
      181 cccgccgagg ccggcgagcg catcgcccag gccatgatcc tccccgtgca gcaggtctcg
      241 ttcctcgaag tggacgagct tggcaccacc gagcgcggca ccggcgggct ggggtccact
      301 ggcagcgggg ccttggtatg aaaatcctga acacagccga actcgtgagt tttgaagaag
      361 ctgatcgctt gcttgaatac gacccagaaa ccggcaacat ggtgtggcgc gtcagaatgg
      421 gggcgcgcac gcttgccgga gaccccgcag gcacagtgca tcacaaaaga agcctgcagg
      481 tttgcatagc cggtcggatt taccaaagcc accggatagc ctggttgctg gcgtatcgcg
      541 agtggccgca gggatgcgta gaccacatcg acggaaactc gcttaacaat aggctcgaca
      601 acctacgaga cgtatctcac cgtacaaact gcgaaaacaa acacaggccg tctcgcagca
      661 acacgtctgg gtaccttggt gttagccgat tcagggacaa atggcaggcg caaatcaagg
      721 tgaaaggcaa agtgataccg ctcggcagat ttgaagaccc agtagtagcc cacgaagctt
      781 acttagccgc taaacgcagc ttgcacatag gctgcactat ttgaaacaaa ggccgcttgt
      841 cgcggccttt ttctcgttac gcacatgttg ttcacaccac gcccgtacca gatgctcatc
      901 gcaggccata tcttagatca tccgcgttgc aatgtattcg cctcgatggg ctcggggaag
      961 agtgcatcaa ccatatacgc cttcgacctg ctcaagatgc tcggcgaggc ccgccggctg
     1021 ctggtgctcg cgcccctcgg cgtggcccgc accacttggc cggtcgaggt gcagaagtgg
     1081 agcgagtcgt tcggccacct gtccgttgcc gccgcagtcg gcacgcccga ccagcggctc
     1141 gcggccctgc gcgcgaacgc cgacatcacg acgaccaact acgagtcgat cgagtggctg
     1201 atagagacgg tcggcgacca ctggccctgg gacatggtcg tcgccgacga gagcacgctg
     1261 ctcaaggggc tgcgggtgtc gctgcagacc tcgtcgaagg gcaccgagtt catcgccggc
     1321 caaggcagcg tgcgtgcaaa ggcgctctcc cgcgtcgcgc tgcggcacgt gcgccgctgg
     1381 gtctgcctca ccggcacgcc ggcaccgaac ggcctgcaag acctctgggg gccgacgttc
     1441 ttcgtggacg gcggccagcg cctcggccgc tcgttcaccg ccttccagaa ccgctggttc
     1501 gtgccggagg gccgctcgtc gaacccggcc gcccagaagt acaagccata cccctggtcg
     1561 gagaagcaga tccaggacgc gatcaaggac gtgagcatca cgatcgacgc gaaagactgg
     1621 ttcgacatca aagacccgct cgaatccctc gtgctggtgg acctgccgcc cgcggcgcgc
     1681 aagcactaca acgaggtcga gaaggaactc ttcaccgagc tggcctcggg cgccgaggtg
     1741 gaggtgttca acccggccgg ccgcagcaac aagtgcttgc agatcgcctc cggcttcgtg
     1801 atccacgacg accaggggtc gtgggagaag ctgcatgacg cgaagatcga cgcactcaag
     1861 accgagatca gcgcagccgg tggcgagccg gtgctggtgg cgtaccagtt catcgcagag
     1921 cgcgagcgca tcctgaaggc gttccctcgc gcgaagctga tcggcgatga cccgcagaca
     1981 gaggccgact ggaacaaggg caagatcccg gtcctgctcg cccacccgaa gagcgccggc
     2041 cacgggcgga acctgcagca cggtggccgc acgctgatcg acttcagcag cggctggaac
     2101 ctggagtacg accagcagat cctggagcgg ataggcccga cccgccagat ccagtccggc
     2161 tacgaccgca cggtgttccg ccgccggatc gtggcccgcg acaccgtcga agaactggcc
     2221 gtgatccccc gcctcaagag caaggcggat gtgcaaacag cactgctaaa tgcaatgaag
     2281 caaagaaccg cttgacaaac atttcgcatc tgctaaattg gagtcctgtt ccaacagcaa
     2341 cgaagaaagt agcaccagat gagtacgacg aatagcaaat ggcttcctcc gcagcaagag
     2401 ggctgcggac cgtggatcga gttcaccgga ccgcacggcg agcgccccgt gccgggagac
     2461 accctcgtcg ggtacctgct ggccgacgag cgcgaagcgc agcaatacaa tcattcagta
     2521 cgtcgcgctg acaggtactc atggggagat aatatctgcg ccgcctactg cgtgaagctc
     2581 gacggcctgc agccccagca acccgctgcc agcgaccggc cggccgcgac cccggagcaa
     2641 gcccccgaca ccgccctcgc cgtccaggtg gccggcgacc actacaagcg cctcgcgatc
     2701 cagccgatcg agtacatcca cgccaacggc atcggcttcg ccgagggcag cgtcatcaag
     2761 tacgtgaccc gctggcgcga caaggccggg atcaccgacc tgaagaaagc ccgccacttc
     2821 ctcgatctgc tgatcgaact cgaagagaag aaggcaggtg ccgcgtgatc gacctccaca
     2881 acccctccgg ctgcctgcac gtcgggcgcc gccgcaagac tcgttcctcc acccaacgcc
     2941 gcatcgtcat gaccctcgcc tacaccacac tggccgccgt cctcgcagcc accgccttct
     3001 ccgccagcgc gcagcacgcc gcgaccatca cggtcgtcgg caccgacatc gcgcaggtca
     3061 cgttccggaa cgacggcccg accgccctgg tggccgcgga gttcttcctc ggcacgccga
     3121 accgcggccg tgcgatcttc tcgaacgtcg gctcgcccac gggcgccgct gacttcgcgt
     3181 tcggggacgg cgtccactac tcggtcacga ccttcgactt cggcgcgctg cagcaaggtg
     3241 cgtcgatcac cgagacggtg aacttcgacc agtacgacgc tgccggcgaa ggctctccct
     3301 tcagcacgga cgccaccggc accttcgcct acatggcgag ccgcatgacg ctggtctggg
     3361 cggacggctt cgagaagatc gtgccgacga gccgcgcgcc gatcgacgcc gtgttctcgt
     3421 tcaccgacat cccgccggtg ccgcccgtct ccgagccggg caccgcggcc ctcacgctcg
     3481 cgggcctcgg cgcactggcc gtggctctgc gcaagaaggg cggtgcagca tgaagatcga
     3541 cgacatcagt ctcgccatcg ccgaggccga gcggttcatc gagcgcgcat gcgtgctgcg
     3601 cagcacactc gttgccgacg agacgcgcgt cgaagcagag ctcgctgcca atccggcggc
     3661 ctacgcctac caccactcgt tcccgaagga acaagggtct gtccgccgcg cgagcatgga
     3721 cctgacccgc gcgctggcga agatgcggag gccgggacga agtacacccc cgcccaactg
     3781 cgcgagatgg cgcagaccgc actcgccgcg aagctgaccg gcgacccccg gtggctgcaa
     3841 cttcaactca ccctggccgt ggtctgcggc ctggaccccg cgaacgtcga ggcgcagatc
     3901 gaagccttgg cgcagccgga gaaggtacat ggctgacaac atcgaacgaa agccgacggc
     3961 agaagagatc cttgactacc tggatgagga caagggaaag accgtagtcc acttgggtgc
     4021 gcgctggtac acgaggccga actacgggca gccgctccgt aggcgactgt cgctgcgtga
     4081 cgccgcaagc gtcgccatgt cctggaagga gaccaagaat ggctgacgac ctcgacctcg
     4141 ccgccgagcg cgaagagatc gcgcggaccg cggccctcag ccaacggcgc cccgcggggc
     4201 cgcaggccac gggcttctgt ctgtggtgtg aagagcccgt cgcgccgctg gtgcgcttct
     4261 gctgccccga gtgccgcgac cagttcgaag cggcaaccac acgcaagcgc caactcggcg
     4321 cgagaaactg aaagcgagtt tgtgatgagt acgacgaata gcaaatggct tccaccccaa
     4381 ctggccggct tcggtccatg gatcgaacgg actcggggta gcggcgctcc ggtgctgtcc
     4441 ggagaggtcc ggatctgcac aagttgggag cggcaaattc gagaatacgt gccttcgact
     4501 ggcgacgcag ccggtgtgaa ctgggatggt gagacggctg ccagcgccgt tgtcgcctac
     4561 tgcgtgaagc tcgaagagca accagtcgag ccccagcaac ccgcggccag cggtctgacc
     4621 cgcctcgtcc cgctgcgcca gaaagccttg cacacgctgg aagagaccac gatcagcgac
     4681 ccgcaggtcg cgttctgggg cgtcagcatc acaggcgtgc cgatggtccg cctcgccacc
     4741 ctggccgacg cacagctggt gcaacgcctg gccgacgccg cgttccaggc cggccgtcag
     4801 aaagcgttca gcgacctggc tgactgggtg agcgcgcggc catgacccgc ttcatccgcc
     4861 ctgcggtgtt cgaagcccgg acgggctaca ccatcaaggc gtttgaacgc aagatccaac
     4921 aaggtgttta cagaagcgat ttacgctata caatcaatca catacaacaa gccgacggag
     4981 ggattcgaac cctcgacaaa gggtgtcgta gggtgctgaa gggtagtcga aacaacgact
     5041 tagctaccct gacgtacccc aaaacaccac cattccgcag tgaattccgc agtagaaagt
     5101 gagcaacaag tgaccagtct gtacaccatc ctcgatcacc tgcgaggcga acctgttcct
     5161 gacgactgga cggagatcaa aaacccgcag accggcgcgg ttgtgggctg gatcggcgac
     5221 gacctgaaag aggccctcca gaaccaccct gcgttcaccg gagaagagtt tcggatgatc
     5281 gtgtctggtg ttcggctgag ccccgaagac gtgtccgtgg accctgtcgc gggaacgatc
     5341 accgtcagat caggggctct tcccgcagac agccactgcg acatctacta cgcgaagccc
     5401 ccgacgaaaa agcacaagca agcccagtgg aagcgtgaaa ccgcggcgtt cggccgcaga
     5461 aagtgagcaa caagtgagca agatccaacg acgcaacaac caggggggtg tgacccacgt
     5521 cgccgacctc ctgccaacgg gccgcgccct ggcgccccgc gagccggtgc ggtgcgacct
     5581 gccgccggtg aatccggcca cggggaggcc gtacttcacg ctgcagcgcg agccgatggg
     5641 ctccggctgg aacggggcgc agaaccccgt ggacgccgcc aagctggcgt tcgaccgcaa
     5701 gatggccgac gccttgcgcc acgacagcga ttcggagggc ggtcatgccg actgacctca
     5761 agcccttcct ccagacctgc cgcgacgtgg ccgcgagcct caagcccaac ggcgaggact
     5821 acgacaacct gctgtacttc ggcgagtgtc tcgaagacgc ctggaacgcc atcctgccgg
     5881 tcgtgtgccg cgggctgctg ctggtgacgt gcccgatctc cgtcccggtc tgcgcctacc
     5941 tgctgcgcaa gtgggcgcgg gaagacgagg cccgccggac cgcgcgccgc gcgctggctg
     6001 ccgccgcggc gcgtggcgtg gggtcttttc aggaaggcgg gcgccatgag cgacaagccg
     6061 actgacctct gctccctctg cggcggctcc ggccacaagg ccaaccgctg cccgtggctc
     6121 gcgtaccgcg agtcggcagg cgagtaaccc ctcctgcctg tcgggctctt caagcggctc
     6181 gacacccccg gccggctccc tacaatcgcc ggccacgtct cccgattctc tcgcaaggga
     6241 cggcgcgcaa gcagccgtgc cttgtctcgc accccaaccc tgttacacca tgagcaacac
     6301 cccttcggcc cgcgcccaga tcatcacccg gcgcacctac aaccgccccc tggacgagga
     6361 cggcactgtt ttcgagacgt gggcacagac cgtggaccgc gtcatccgcc accagaagtg
     6421 gctgtgggag cgcgcccaga agcgcgccat caacttcgcg cagggcgagg aactggaaga
     6481 actccgcgaa ctgctgatgg aacgcaaggg cctgatgtcc ggccgcaccc tctggctggg
     6541 aggcaccgac atcgccaaga cccgcatggc ctcgcaactg aactgcacgt tccggcgagt
     6601 agctacggtc cacgacgcag ttgatgtgtt ctggttgctg ctgcaaggcg ccggcacggg
     6661 ttttgacggc acccctggca cgctgaacgg cttcagccgc aagttggacg tgcagatcat
     6721 ccgctcgacc cgcaccctgg agcaatgggc cgccggcgag cgcggcgacc cgatgaacca
     6781 cgagttcgtc gacggagaca cgtggaccat ccggatcggg gacagcgccg ccgcgtgggc
     6841 gaaagcagtc ggcaaactcc tggccggcaa agctgtcgtc aagacccttg ttctcgacct
     6901 gcgcgagatc cgcgccagcg gcgtgcggct gaagggctac ggctgggtga gcagcggcga
     6961 cggcccgctc tcgaaggcac
//
LOCUS       DBNHOX010000530         4504 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1199903, whole genome shotgun sequence.
ACCESSION   DBNHOX010000530 DBNHOX010000000
VERSION     DBNHOX010000530.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1199903"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1992)
                     /locus_tag="AC16UZ_11645"
     CDS             complement(184..1992)
                     /locus_tag="AC16UZ_11645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR6247380.1"
                     /translation="MSISTNHSLASNAALLKSKASKYAYYGVVIAVVAIVVATGLVSV
                     YNTGGISLDSLIEAQRENKALWLLDLMPFAFAFWGQYVSSMIAYEAGALVVDQTNELR
                     AQTTAIEQQAMHDLTHDKLTDLPNRALLRDRLEQAVSAAAHERSQLAILVLDLDRFKE
                     INDTLGHYNGDRLLKQVALRLEGAVKETDTVARLGGDEFAVLLHRISSPDDATDLGRK
                     IGKALQAPFGLEGLKLDVQASIGIAVFPDHGPDADTLLQRADVAMYIAKQQKSGCVSY
                     DPKLDKHSPRRLTLMGELRHAIDADELVLHYQPKIDIRSGTIVEAEALVRWQHPQYGM
                     LGPDEFIPLAERTGLIKPLTIWVLGRALRQQESWRGQGIDIGVAVNMSAHGLFDLELP
                     DLVAGLLAARGTAPDRLMLEITETSIMVDQQRALDILSRLADMGVRLSIDDFGTGYSS
                     LAYLSKLPVDEIKIDRSFVMDMIENAKHAMIVRATVDLAHNLDLEVVGEGTCEPEILE
                     HLRELGCDAAQGNYISPAVDVASFSQWLQNSQWKPRRTNPRQSTSETSGWPGRAKPRA
                     VAHTDLESIESAIQDLEEFVRQVDPKPDGSKAAGAS"
     gene            2330..4204
                     /locus_tag="AC16UZ_11650"
     CDS             2330..4204
                     /locus_tag="AC16UZ_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6247381.1"
                     /translation="MQQRIGKRDLILYAFMVAVLISIWLAMVQIDRQWQFIALTQEKL
                     DDQTRDIADLRRQFRRGISVSTETAGAGDPNALPDSWRGFKRAVAATEMPDYARGDWL
                     VYSFSSQVPSLTPYMSGDAYASSIQQWIIDGLVTRDPVTLEWLPHVAESWNISEDGLT
                     ISFVVRDGVVFSDGEPLTAEDVAFSWRFVMDEKIAAPRARAYLSRIDDVTVDGRTVTF
                     RFTEPYFGSFSLAGNLGILPRHFYSKYLESVAAAEEYNTSTGLLLGSGPYRLPSPTAW
                     KPGDLIELVRNERYWGWVPAPFDRRIWKTINSDAAQLTEFKNGGIDVYAALPLEYRDL
                     TQNEEIMKRTRSYEYYNPRGGYSYIAWNNLRDGKPTAFADRRVREAMTYLTDRERVID
                     EVLLGYAQPANGPFNPLGKQADPSLSVRGYDLDKAKALLAEAGYLDRDGDGVLESEAG
                     EPLAFKLTYPSGSDNYKRIMLLLKDLYVQAGVLMEPDPVDWPVLINTLNTKNFDAVSL
                     AWTSGFEIDIYQMLHSSQTEPGGDNFISYINPGLDELIETARQEMDEEARMAIWHQVH
                     REIWAEQPYTYLFRTKSLIFADRRIRNVQVVRAGLNEGGLWNMPMEWYVPTDAQKYSD
                     "
     gene            4238..>4504
                     /locus_tag="AC16UZ_11655"
     CDS             4238..>4504
                     /locus_tag="AC16UZ_11655"
                     /inference="COORDINATES: protein motif:HMM:NF040248.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247382.1"
                     /translation="MVNYVIRRLLLIFPTLLGITILVFAVMAAAPGGVGASLLSRSGD
                     LRPEERAAMERYINERYGLDKPMPMQYLRWLNNVSPIGFRKDENN"
BASE COUNT          860 a         1463 c         1394 g          787 t
ORIGIN      
        1 gtcgatgtga tgcgttgccg ggatattgcg acctggttcg tacaacaacc ggaaactggc
       61 gcacaacgtg caggggggta gttaacagct gcgccgagaa ccggcgcaga ggcggacaaa
      121 tcccttacat cgcggaaaac atatagacaa acggactatc ggccaggccg atgacggtgc
      181 gtgtcagctg gcgcctgccg ccttggagcc gtcaggcttg ggatcgacct ggcgcacgaa
      241 ttcctccagg tcctggatgg cggattcgat ggattcgagg tccgtatggg ccaccgctct
      301 gggcttggca cggcccggcc aaccggaagt ctccgacgtc gattgacggg gattggtccg
      361 gcggggcttc cattgggagt tctggagcca ctgggagaag ctcgcgacgt ccacggccgg
      421 gctgatgtag ttgccttggg cagcatcaca ccccagctcg cgcagatgct ccagtatctc
      481 cggctcgcag gtgccctcgc ccaccacctc gaggtcaaga ttatgagcaa gatcgacggt
      541 cgcccgcacg atcatggcgt gcttggcatt ctcgatcatg tccatcacaa aggatcggtc
      601 gatcttgatc tcgtccacgg ggagcttgct gaggtacgcc agggaggagt aaccggtgcc
      661 gaagtcgtcg atggacaggc gcacccccat gtcggccaga cgcgagagga tgtccagagc
      721 ccgttgctgg tccaccatga tggaggtttc ggtgatctcg agcatcaggc ggtcgggcgc
      781 agtgccgcga gccgccagga ggccggccac gaggtccggc agctcgaggt cgaacagccc
      841 gtgggccgac atgttgacgg cgaccccgat gtcgataccc tgcccgcgcc aggattcctg
      901 ctgccgcagc gctctgccca gcacccagat ggtcaacggc ttgatcagac ccgtgcgctc
      961 ggccagcggt atgaactcgt cgggccccag catgccgtac tgaggatgct gccagcgcac
     1021 cagcgcttcc gcctccacga tcgtaccgga gcggatgtcg atcttcggct ggtagtgaag
     1081 caccagctcg tccgcatcga tcgcatgacg cagctcgccc atgagggtaa ggcgccgggg
     1141 gctgtgcttg tccagcttgg gatcgtagga cacgcacccg ctcttctgct gcttggcgat
     1201 gtacatggcc acgtcggccc gctgcagaag ggtgtcggca tccggtccgt gatcggggaa
     1261 aacggcaatg ccgatgctcg cctgaacatc cagcttgagc ccttcgagcc cgaacggcgc
     1321 ctgcagcgcc ttgccgatct tgcggcccag atcggtggca tcatccggag acgagatccg
     1381 gtgcagaagc accgcgaact cgtcaccgcc gagcctggcc acggtgtccg tctctttcac
     1441 ggcgccctcg agacgaagag cgacctgctt gagcaggcga tcgccgttgt agtgcccgag
     1501 cgtgtcgttg atttccttga accggtccag gtccagaacc aggatcgcta gctgcgaccg
     1561 ctcgtgggca gccgcgctga cggcctgctc caggcgatca cgcagcaacg cccggttggg
     1621 cagatcggtg agcttgtcgt gggtgaggtc gtgcatcgcc tgctgctcga tggcagtggt
     1681 ctgagcgcgc agctcgttgg tctgatcgac caccaacgcg ccagcctcgt aggcgatcat
     1741 ggagctgacg tactggcccc agaacgcgaa cgcaaagggc atgagatcga gaagccaaag
     1801 ggccttgttt tcccgctggg cctcgatgag cgaatcgagc gagatcccgc cggtgttgta
     1861 gacggagacc agccccgtgg cgaccacgat ggcgaccaca gcaatgacga cgccgtagta
     1921 ggcgtacttg ctcgctttgc tcttcagcag tgccgcgttg ctcgcaaggc tgtggttggt
     1981 gcttatcgac acgccaactc ctacgtttgt ggcggacgcc ccactggaac gcaaccgcta
     2041 actttcccgg atgttagacc aaccggccgg tactcaaggt gacgaccttc aaatatccag
     2101 gcaacgaatc tgctttgtag gctcgtgacc gggcgcccaa gggctcgggc aagtcgaccc
     2161 atgtccatat tgtatacaaa attgcctatt tagccatccg cgcccgggct tgcgtaaaaa
     2221 cacagtcacc gggggcccaa aaggcctcgc gccggttgcg gcccgggaca cgcgggttag
     2281 acttggcgct tcgccgacac cggcccccac tcagcggaaa tcgacccctt tgcagcagcg
     2341 catcggcaaa cgcgacctga tcctctatgc cttcatggtg gcggtgctaa tcagcatctg
     2401 gctggccatg gtccagatcg accgacagtg gcagttcatc gcgctcaccc aggagaagct
     2461 cgacgatcag acccgcgaca tcgcagacct cagacgccag ttcaggcggg gcatttccgt
     2521 ctctacggaa acggccggtg caggagaccc gaatgccctg cccgactcct ggcggggttt
     2581 caagcgggcc gtagcggcca ccgagatgcc cgactatgcc cggggcgact ggctcgtgta
     2641 ctcgttttcg agccaggttc cgagcctgac accctatatg tccggtgacg cctacgcgtc
     2701 gagcattcag cagtggataa tcgacggcct ggtgacccgg gacccggtca ccctggagtg
     2761 gctgccccac gtggccgaat cgtggaacat cagcgaggac gggctgacga tcagcttcgt
     2821 cgtccgcgac ggcgtggtct tctccgacgg cgagcccctc accgcggagg acgtggcttt
     2881 cagctggcgc ttcgtcatgg acgagaagat cgccgcgccg cgggctaggg cctacttatc
     2941 ccgcatagac gacgtcaccg tcgacggccg cacggtgacc ttccgcttca cggagcccta
     3001 cttcggttcc ttctctctgg ccgggaacct gggcattctg cccaggcact tctactcaaa
     3061 gtatctggag agcgtggccg cagccgagga gtacaacacc tccaccgggc tgctgctcgg
     3121 ctcggggccg taccggctgc cctcgccaac cgcctggaag ccgggcgatc tcatcgagct
     3181 ggtgcgcaac gagcgctact ggggctgggt acccgcgccc ttcgaccggc gtatctggaa
     3241 gaccatcaac tccgacgcgg cccagctcac cgagttcaag aacggcggca tcgacgttta
     3301 cgccgcgctg cccctggaat atcgagatct cacacagaac gaagagatca tgaagcgaac
     3361 caggtcgtac gagtactaca acccgcgcgg cggttactcc tacatcgcct ggaacaacct
     3421 gcgcgacggc aaacccaccg cgttcgccga tcgcagggtc cgagaagcca tgacctacct
     3481 gacggaccgg gagcgcgtca tcgacgaggt cctgctcggc tacgcccagc ccgccaacgg
     3541 cccgttcaac cccctgggca agcaggccga cccgagcctg tccgttcgcg gctacgatct
     3601 ggacaaggcg aaggccctgc tcgccgaagc cggctacctg gaccgcgacg gcgatggggt
     3661 cctcgagtcc gaggccggcg agccgctggc cttcaagctc acctacccct ccggcagcga
     3721 caactacaag cgcatcatgc tgctgctcaa agacctttac gttcaggccg gcgtgctgat
     3781 ggagcccgac ccggtggact ggcctgtact gatcaacacg ctgaacacca agaactttga
     3841 cgcggtgagc ctcgcgtgga catccggatt cgagatcgac atctaccaga tgctgcattc
     3901 ttcgcagaca gagcccggcg gggacaactt catcagctac atcaacccgg ggctggacga
     3961 gctcatcgaa accgcccgcc aggagatgga cgaagaggcc cgcatggcca tctggcatca
     4021 ggtgcaccgc gagatctggg cagagcagcc ttacacctat ctgttccgga ccaagtcgct
     4081 gatcttcgcg gatcggcgca tccgcaacgt gcaggtggtt cgcgccgggc tcaacgaggg
     4141 cggactgtgg aacatgccca tggaatggta cgtgcccacc gacgcgcaga agtacagcga
     4201 ctgacggcag gctgagaagg aagcagagcg caacacgatg gtcaactacg tcatccggcg
     4261 cctgctgctg atattcccga ccctgctggg catcaccatc ctcgtcttcg ccgtgatggc
     4321 ggctgccccc ggtggcgtgg gcgccagcct gctctcccgc tccggagatc tgcgacccga
     4381 ggagcgcgcc gccatggagc gctacatcaa cgagcgttac ggcctggaca agccgatgcc
     4441 catgcagtac ctgcgctggc tcaacaacgt ctcgcccatc ggcttccgca aggacgagaa
     4501 caac
//
LOCUS       DBNHOX010000531         2190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_172514, whole genome shotgun sequence.
ACCESSION   DBNHOX010000531 DBNHOX010000000
VERSION     DBNHOX010000531.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_172514"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="AC16UZ_11660"
     CDS             <1..383
                     /locus_tag="AC16UZ_11660"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR6247383.1"
                     /translation="EVNADNVRAARGNIAHAGLDGRVEVVHGSSSDVIPTLEGSFDLV
                     FLDHWKDLYLQDLQLIETQGLIAPGSVVVADNVGELFGADAYLDYVRHCGRYDSANRP
                     ATIEYTSLPDAVEISVYRGQGDDG"
     gene            373..1479
                     /locus_tag="AC16UZ_11665"
     CDS             373..1479
                     /locus_tag="AC16UZ_11665"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6247384.1"
                     /translation="MTDEPIAPVTVYGSSISYFTGKLENYLRIKGIPYELKPLGPGSV
                     RKLIKSRTGSSQMPAVRLGDGRWLTDTTPVIGWFEREYPEPAVIPHDPVQRFFSLLLE
                     DYADEWLWRPAMHYRWFYDVGARFASAHLAQELTRGVYLPMFVKRRAITRRQRNGYTR
                     GDGITADQVPGVEAIYSRTLGQLETIFRSRPFLLGASPSLADVGFSGPFFRHFGLDPV
                     PAEIMRRDAPAVYEWIARLWNAGPVDSSMAIPAGIPDDWAPILDEIGTHYLPYLCANV
                     DAVAAGLRRFDAEVGGVRYHRARWSPYRVWCLKKLRETFAGLTEDEQAEARTLLERHG
                     CWEPMWRHASLPLKDGFCERLPFHADAKMIDVYQ"
     gene            complement(1503..>2190)
                     /locus_tag="AC16UZ_11670"
     CDS             complement(1503..>2190)
                     /locus_tag="AC16UZ_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6247385.1"
                     /translation="PRSSVSSPPDAVSPLRLIPSAPSFHVAVVLPLYLIVLLLWAASA
                     SAAQADWEPVRKVHGIQVEGLMMPSGFRSHRAQVAVCTDLADLRAFISDTSRFHEWIP
                     DLAEVRLLEQSDDGVVYYMRSKAPWPVKDRDMVYRLFGVPESGDAASVALAIEGLPGR
                     LPEVDGAYRMHDVSGQWRIWERGDRLDVQQQIYLNPGDVPRMFANRSMSASLARTLGN
                     LASRYPCPKS"
BASE COUNT          399 a          721 c          704 g          366 t
ORIGIN      
        1 tagaggtgaa tgccgacaac gtgcgggccg cacggggcaa catagcgcac gcgggtctcg
       61 atgggcgcgt agaggtggtg cacggcagct cgagcgacgt gatcccgaca ctcgagggga
      121 gcttcgatct cgtgtttctg gatcattgga aggacctcta cctccaggat ctgcagctga
      181 tcgaaaccca agggttgatc gcgccgggct ccgtcgtggt agcagacaac gtgggcgagc
      241 tgttcggcgc ggacgcttac ctggactacg tccgtcactg cgggcgctac gattcggcca
      301 accgcccggc caccatcgag tacacgtcgt tgccggatgc cgtagagatc tcggtatacc
      361 gggggcaagg cgatgacgga tgagcccatc gcgccggtca ccgtttacgg gtcgagcatc
      421 tcgtacttca cggggaagct ggaaaactac cttcgcatca agggcatacc ctacgagctc
      481 aaacccctgg gccccggctc tgttcgcaag ctcatcaaaa gcagaaccgg atcctcgcag
      541 atgcccgcgg tacggcttgg ggacgggcgc tggctgaccg ataccacgcc cgtcatcggg
      601 tggttcgagc gggagtatcc cgagccggcg gtgattccgc acgatccggt gcagcggttc
      661 ttcagcctgc tgctggaaga ttacgccgac gagtggcttt ggcgaccggc catgcactac
      721 cgctggttct acgacgttgg cgctcgcttc gccagcgccc atctggccca ggagctcacg
      781 cggggtgtat acctgcccat gttcgtcaag cgtcgcgcca tcacccggcg tcaacgcaac
      841 ggctacacca ggggagacgg catcaccgcc gaccaggtcc cgggtgtgga ggccatctac
      901 tcgcggaccc tgggacagct cgaaacgatc ttccgatccc ggcccttcct gctgggtgcc
      961 agccccagcc tggcggacgt cggcttctcc ggccccttct tccgtcactt cgggctcgat
     1021 ccggtccctg ccgagatcat gcgcagagac gcgcccgcgg tctacgagtg gatcgcccgg
     1081 ctgtggaacg ccggccccgt ggactccagc atggcaattc cggccggtat tcccgacgac
     1141 tgggcgccca tcctggacga gatcggcacg cactacctgc cctacctctg cgccaacgtg
     1201 gacgcggttg cagcgggcct gaggcgcttc gacgcggagg tcggcggcgt gcgctaccac
     1261 cgtgcgcgct ggtctcccta tcgggtctgg tgcctgaaga agctgcgcga gacgttcgcg
     1321 ggcctcaccg aggatgagca ggccgaagcc agaaccctgc tcgagcggca cggctgctgg
     1381 gagccgatgt ggcggcacgc ctcgctgccg ctgaaggatg gtttctgcga gcggctgccc
     1441 ttccacgccg atgccaagat gatcgacgtg tatcaatagc cgcgcgacgt cgctgctatc
     1501 cttcaactct tcgggcaggg gtatcgcgag gccagattgc cgagcgtcct ggccaggctg
     1561 gccgacatgc tgcggttggc gaacatccgc gggacgtccc cggggttgag gtagatctgc
     1621 tgctgcacgt cgagccggtc accccgttcc catatgcgcc attgaccgga aacgtcgtgc
     1681 attcgatagg cgccatccac ctcgggcagc cgcccgggca ggccctcgat ggcgagcgcc
     1741 acgctggcag cgtccccgga ctcgggcacg ccgaagagcc ggtacaccat gtctcgatct
     1801 ttcaccggcc agggcgcctt gctcctcatg tagtagacga ccccgtcgtc ggactgctcg
     1861 agcaggcgca cctcggcaag atcgggaatc cactcgtgaa atcgcgaggt gtcggaaatg
     1921 aacgcacgca gatccgcaag atcggtgcaa acggcaacct gagcacgatg ggaccggaaa
     1981 ccggacggca tcatcagacc ctcgacctgg ataccgtgca ccttccgtac aggctcccaa
     2041 tcggcctgcg cggctgaagc ggacgcggcc cagagcaaaa ggacgatcag atacaggggc
     2101 aaaaccacag ccacatgaaa ggacggcgcc gatggtatca gccgcagggg actcaccgca
     2161 tccggcggcg agctcaccga tgaccgaggg
//
LOCUS       DBNHOX010000532         3505 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1456414, whole genome shotgun sequence.
ACCESSION   DBNHOX010000532 DBNHOX010000000
VERSION     DBNHOX010000532.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1456414"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="AC16UZ_11675"
     CDS             <1..345
                     /locus_tag="AC16UZ_11675"
                     /inference="COORDINATES: protein motif:HMM:NF016121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR6247386.1"
                     /translation="GLLVRYWQAGSRTAWILDEIGKDLPITAGIVINDGAIEDVRVLE
                     FRESRGWEIRYPFFTRQFQNARLQGDRLTQNIDGITGATLSVRAMKRMATAALLLHEH
                     SSASSTTLAKAP"
     gene            386..1039
                     /locus_tag="AC16UZ_11680"
     CDS             386..1039
                     /locus_tag="AC16UZ_11680"
                     /inference="COORDINATES: protein motif:HMM:NF015863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR6247387.1"
                     /translation="MLVLVYLLATGVPLQYAAELDLGQRHVASEWVLDWYGLQAPDEA
                     TRSGPAVMLGGTLFLDGNEQAGLERLVGAIPAGGILAVASSDEVWLFHPGVPDPERTA
                     FRPNIRRIGSLDGQIYLDTTEGLLAADGLLMNWTPTNAPAGAIEWGRVTPLSAGEAAA
                     YRQRFRGRMLTAERWLQDLHSGRFFGTPGVIIVDVASLLMLILAGTGIVLWWRSRRG"
     gene            complement(1064..1882)
                     /locus_tag="AC16UZ_11685"
     CDS             complement(1064..1882)
                     /locus_tag="AC16UZ_11685"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6247388.1"
                     /translation="MGGYTTREVSDLIGLKPDQVRHYVRRSLLRPARGDRGEYRFNFQ
                     DVVLLRSAKGLLDADVSSRKAYRVLLKLQSELSQVKSLAAVRIFADGNNVVVREDNQV
                     WDVETGQGQLDFTVRELADNVATLAQRNLIVLRESDGLDSDEWYNLGLDLEEVDPDRA
                     PDAYSRAIELNPKNADAHVNLGRLMQLKGNLKFAKRHYELALVAVSNHQLAYYNLGTV
                     YDELDDMDKAAEYYRRAPGVPDAHFNLSRIYQMQGDEVSALRHMRQYRELLDVE"
     gene            complement(2104..2946)
                     /locus_tag="AC16UZ_11690"
     CDS             complement(2104..2946)
                     /locus_tag="AC16UZ_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010448255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HIR6247389.1"
                     /translation="MSRQASPAGVDKARLEAFLARHRHLLVITGAGCSTPSGIGAYRD
                     EAGEWLRAEPVQMRDFVASEAARRRYWARSMHGWRAFRDAAPNAAHQALADLERRGVV
                     RGLITQNVDGLHQRAGHDDVIELHGSLRRVVCLECGVSREREEVQRWLEARNGFVLDA
                     SASLAPDGDADLVIDDLSAFEVPRCADCGGLLKPDVVFYGDSVPRDRVDACYAGVAQA
                     DAVLVVGSSLMVFSSFRFVRRAVDLGKPVAAVNRGRTRADAWLEFKVDEDCAAVLERI
                     AANL"
     gene            complement(2943..>3505)
                     /locus_tag="AC16UZ_11695"
     CDS             complement(2943..>3505)
                     /locus_tag="AC16UZ_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR6247390.1"
                     /translation="IHGRVRPPRATEPWQETLLEWVDAALGQLGVELAEFSAAMDVFS
                     EDFDWNADETAQAIIPAANGQFTARSLARMYAMIAQGGELDGVRVLSEGRVREITEVR
                     SRTRDRVLFIPMHWRMGYHRAFAFGVSAPHAFGHYGYGGSGAFCDPSRELAVALILNS
                     GAGTPMGGRYMPLVARAAIRAADRCR"
BASE COUNT          593 a         1188 c         1150 g          574 t
ORIGIN      
        1 gggctgctcg tacgctactg gcaggcggga tcccggactg cctggatcct ggatgagatc
       61 ggcaaggacc tgcccatcac ggccgggatc gtgatcaacg acggcgccat cgaggacgta
      121 cgggtcctcg agtttcgcga aagccggggc tgggaaatcc gctatccgtt cttcacgcgg
      181 cagttccaga atgcccggct ccagggcgat cgcctgaccc agaacatcga cggtattacc
      241 ggcgccaccc tctccgtccg ggccatgaag cgtatggcca cggccgcgct tctgcttcat
      301 gagcattcca gcgccagcag caccacgctc gccaaggcac cctagacggg tcaatcaatg
      361 gcacagaacc gcgggcatct gcgcggtgct cgtgctcgtc tacctgctcg ctaccggggt
      421 gccgcttcaa tacgccgcag agctggacct cgggcaacgt cacgttgcca gcgagtgggt
      481 gctcgattgg tacgggctac aggcgcccga tgaggcaaca cgcagcggcc cggccgtgat
      541 gctcggcgga accctgttcc tggacggaaa cgagcaggct gggctcgaac gcctggtggg
      601 cgcgataccg gcgggcggga ttctggccgt ggcgagttca gacgaggtct ggcttttcca
      661 ccccggggta cccgacccgg aaagaacggc attccggccc aacattaggc gcatcggcag
      721 cctggacggt cagatctacc tcgacaccac cgagggtctg ctggcagcag acggtctgct
      781 gatgaactgg accccgacga acgcgcccgc cggcgccatc gagtggggac gcgtgacgcc
      841 cctgagcgct ggggaggcag cggcctaccg gcagcgattc cggggcagga tgctgaccgc
      901 ggagcgctgg ctgcaggacc tgcacagcgg gcggttcttc ggcacgccgg gcgtgatcat
      961 cgttgacgtg gcgtcgctgc tgatgctgat cctggcagga accgggatcg tgctgtggtg
     1021 gcgttcgaga cgcggctgag agccgtgccg gaccggccga cggtcactcg acgtcgagca
     1081 actccctgta ctggcgcatg tgcctgagcg cagacacctc gtcaccctgc atctggtaga
     1141 tgcgggagag gttgaaatgc gcatccggca cgccgggcgc acgccggtag tactcggccg
     1201 ccttatccat gtcgtcgagc tcgtcgtaaa cggttccgag attgtagtag gcgagctgat
     1261 gattcgagac cgcgacgagg gcaagctcgt agtgtcgctt ggcgaacttg agattgccct
     1321 tgagctgcat gagccgcccg aggttcacgt gggcgtcggc gttcttggga ttgagctcaa
     1381 tggcccggga gtatgcgtct ggagcgcggt ccggatcgac ctcctcgagg tcgaggccca
     1441 ggttgtacca ctcgtcgctg tccagaccgt cgctttcgcg caggacgatg aggttgcgct
     1501 gggccagggt cgccacgttg tccgccagct cccgcacggt gaagtccagc tgcccctgcc
     1561 ccgtttccac gtcccagacc tggttgtctt cccggaccac cacgttgttg ccatcggcga
     1621 agatgcgcac cgctgccagc gatttgacct ggctgagctc cgactgcagc ttcagcagta
     1681 cccggtaggc cttgcgcgag gaaacgtccg cgtcgagcag acccttggcg ctgcggagaa
     1741 gcacgacgtc ctggaaattg aagcggtact cgccgcggtc tccacgagcc ggccgcagga
     1801 ggctgcgccg aacgtagtga cggacctgat cgggcttaag cccgatgaga tcagagactt
     1861 cccgagtggt atacccgccc atcagattgg cagatgcggt tccttcattg gtgcctgacc
     1921 gaggtgtcta gccacggctt cccgcctctt cgagaccgcc ggcggcaggg aacgccggca
     1981 ctatagcgta ggggtggtga gccctcaaca cgggccgggc aaagtccccg ggcggaggcc
     2041 cgggtggagg cgcgtctgcg agccctgctg cgagccgggc cggaagcccg ccacaaggcc
     2101 cacctagaga ttggcggcga ttcgttccag cacagcagca cagtcttcgt cgaccttgaa
     2161 ctcgagccat gcatccgccc gggttcgccc ccggttcacc gcggccaccg gctttcccag
     2221 gtccaccgcg cgacgcacga aacggaagct cgaaaagacc atcagggacg agcccacgac
     2281 gagaacggca tctgcctggg caacgcccgc gtagcacgca tccacccggt cgcggggcac
     2341 ggagtctccg tagaacacca cgtccggctt gagcaagccg ccgcagtctg cacagcgtgg
     2401 cacttcgaag gcggagagat cgtcgatgac gaggtccgca tcgccgtcgg gcgcaaggct
     2461 ggcactggca tcgaggacga acccgttgcg ggcttccagc catcgctgca cctcctcgcg
     2521 ctcgcggctc accccgcatt cgaggcagac gacccgccgc aggctgccgt gcagctcgat
     2581 gacgtcgtca tgtcccgccc gctggtgcag cccgtcgacg ttctgggtga tcagtccccg
     2641 cacgacgccg cgcctttcca ggtctgccag cgcctggtga gcggcgttgg gtgcggcatc
     2701 gcgaaacgcc cgccagccgt gcatgctccg ggcccagtag cgccgacgcg ctgcctcgct
     2761 ggcaacgaag tcacgcatct gaaccggctc ggcacgcagc cattctcccg cctcgtcccg
     2821 ataggccccg attcccgacg gcgtgctgca acccgcgccg gtaatgacca gcagatgccg
     2881 atgacgggcg aggaatgctt ccagccgggc tttgtcgacg ccggccgggc tggcttgccg
     2941 gctcaccggc accgatcggc ggcgcgaatc gcggcgcgcg caaccagcgg catgtagcgg
     3001 ccgcccattg gggtacccgc tccgctgttc aggatgagcg ccaccgcaag ctcccggctc
     3061 ggatcgcaga acgctccgct cccgccatac ccgtaatgtc cgaacgcgtg gggcgcgctg
     3121 accccgaacg cgaatgcccg gtgatagccc atgcgccagt gcatggggat gaagagcacg
     3181 cgatcacggg tacggctgcg gacctcggtg atctcgcgga ccctcccctc ggacaggacc
     3241 ctgaccccgt ccagctcgcc gccctgggcg atcatggcat acatgcgcgc aagcgagcgg
     3301 gccgtgaact gaccgtttgc cgccgggatg atggcctgcg ccgtctcgtc ggcgttccag
     3361 tcgaagtctt cggaaaaaac gtccatggcc gcgctgaatt ccgccagctc cacccccagc
     3421 tgccccaacg ccgcgtcgac ccattccagc agggtttcct gccacggctc ggtggctcta
     3481 ggcggccgga cgcgcccgtg gatca
//
LOCUS       DBNHOX010000533         5824 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1200030, whole genome shotgun sequence.
ACCESSION   DBNHOX010000533 DBNHOX010000000
VERSION     DBNHOX010000533.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1200030"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2406
                     /locus_tag="AC16UZ_11700"
     CDS             <1..2406
                     /locus_tag="AC16UZ_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6247391.1"
                     /translation="VTAIGQSTIEGAGVERVEDFIRLTPGVTLVNTAEVGDTQVNIRG
                     INGARDAENSFAFIVDGILHTNPAAFNREYPGLQQIEVFKGPQGAIYGRNAAAGAVIV
                     TTERPTEEFTGSIKTSYANDDSLFVQGTVSGPIVEDELFFRLSGTYRDTDGFWRNEFK
                     NDSSTIDRFKNYDIDGRLLWEPNDRLSVDMKGRYGEVDASAITFNATFHLPVLAGGEP
                     ANPAAFEDVNDHKFIFQHNVDSDNNQEALELSVKADYEFENGYTLTGWMLYSDIQNDF
                     IADGTSAAFGFFQGNDACLDSTATLNAQGVTLPPPQILGASPVGIIFTPDFSGSFFGP
                     YTPTTCDGIQEQVRDQDDFSFELRLASPVDQALRWMGGVYFLTIDRQVGISLNTDSGE
                     KPIRGLLQTSGPNRTEALVYDDFESDVFAVFGQLEYDITDAVELSFALRYDREERDVS
                     NLVPTDVYSTVIDLVPGDEDPLNPGLIGGAIPDKSKTFEEIQPKVALTWDVTEDFTIF
                     GTWGKGFKAGGFNNQGSQATVDGFINGLINGDVLGGPAFADILGVPLPQINDDFEEET
                     SSAFEVGFKSQLWDGRLSLEGAAYYVEVDDMQFFEFFVGTFGLLRVVSNLDEVEIYGF
                     ELAATAQLHDYVSVYAGANIIESEIKDNSARPDTVGNDAPYTPDYTFNVGAELNYPLS
                     GDLNLFARVDAQFVGDTWFHTVQSGQRPTIFSPLFEISIFGEGSGPLGFADFENSKRD
                     AYVTVDVRAGIQGENWSVLGFAKNIGDEKYLEEVIPAPEFGGAFDHPGARRRYGVEVS
                     YRF"
     gene            2437..3639
                     /locus_tag="AC16UZ_11705"
     CDS             2437..3639
                     /locus_tag="AC16UZ_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6247392.1"
                     /translation="MSKPLEGIRLLDLTHMLSGPYAGMILADLGCDTVKVEPPGTGEG
                     TRRLLETDPKNSFKGMGAYFFTLNRNKRSVAIDLKSETGRRYFYELVKVADIVLDNFS
                     ADVTKRLLIDHDSLAAVNPRIVTCSVSGFGEDGPHYRRPAFDQIVQGLGGGMSITGSG
                     PGEAVRAGIPIGDLGGGMFAVMGVQAALLARERTGVGQHVDISMLDVQISLLNYMATM
                     YTLSGEVPGPIGNSHFVHTPYNTFETADDTIIIAVIGDTFWPALCDLLEIDELRKPEF
                     ATAPKRLEHKAYIEGLIGERLLTRPAAEWLEALEAARIPCARVNNIAQALDDPQILHR
                     HMVVEVEHPDGGRVKVPGNPIKLSGTPAETFSPPPLLGAHTREVFAEWAGMAEARIAE
                     GMEAGAIS"
     gene            3672..4577
                     /locus_tag="AC16UZ_11710"
     CDS             3672..4577
                     /locus_tag="AC16UZ_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HIR6247393.1"
                     /translation="MTETVIINDVGPRDGLQNDAADVTPEDRALLINRLIDAGVPAVE
                     VASFVSPRAVPKMAGADRVVAGIDLSRADCSALVPNHKGYELARDAGIRSVAVVLSAT
                     ETMNLKNINMDLETTVRVCEEVVGRAVQDGIHARAYVSVAVECPYEGLVSEETVASLA
                     GRMFDAGAAEVIVADTIGAANPAQVKSLFEKLIGAFGAERLSAHFHDTRALALANAWQ
                     ALECGIRKFDSSIGGLGGCPFAPGAAGNLATEDLASMLEQAGFHTGIDMDSLLSVVAL
                     ARELVGHDLGGRSLAWQLGRKTEAA"
     gene            4580..>5824
                     /locus_tag="AC16UZ_11715"
     CDS             4580..>5824
                     /locus_tag="AC16UZ_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR6247394.1"
                     /translation="MTYKLGVDVGGTFTDVLLIDSESGAVHTAKVPSTPEDSSVGVLN
                     GIARACANAGVDPAAITQVTHGTTVATNAVLTGRGALVGLVTTRGYRQVLQIARSYVP
                     GGLGGWVIYNKSDPMAPLECTVEVPERIGADGSVVEALDEDAARSALRSLGARGIEAL
                     TVSLINSFTNPAHEERLAEIAAEELPGVPVSLSSEVIPEMQEYERTITTVANSYVRPE
                     VSRYIDNLQRELDERMNSPKLSILRSDGGLSTAPAAAKYPVNLLMSGPAGGVAGALWV
                     GRQAGFDNLLTFDMGGTSTDVSLIEQGKAVVRRETTVGDVNVRASSMDVRTVGAGGGS
                     IAYVPELTGALRVGPDSAGAVPGPAAYDLGGERPTVTDANVVLGYLPAEQKLGGDMTI
                     SRDKAAAAVQQIADAMGLSLEAA"
BASE COUNT         1060 a         1821 c         1903 g         1040 t
ORIGIN      
        1 gtgacggcca tcggccagtc caccatcgag ggggccgggg tcgagcgggt tgaggacttc
       61 atccggctca ctccaggtgt gacgctggta aatacggctg aggtgggtga cacccaggtc
      121 aacatccgcg gtatcaacgg cgcccgcgac gcggagaaca gctttgcgtt catcgtcgac
      181 ggcatcctcc acaccaaccc ggcggcgttc aaccgcgagt acccgggcct gcagcagatc
      241 gaagtgttca aagggcccca gggcgccatc tacggccgta acgccgcggc cggggcggtc
      301 atcgtcacca cggagcggcc gaccgaggag tttaccggca gcatcaagac gagctacgcc
      361 aacgatgaca gccttttcgt ccagggcacg gtgagtggcc ccatcgtgga ggacgagctc
      421 ttcttccgcc tgtccggcac ctatcgcgac acggacggct tctggcgcaa cgagttcaag
      481 aacgactcgt cgaccatcga tcgtttcaag aactacgaca tcgacggccg tttgctctgg
      541 gagcccaacg atcgcctttc ggtggacatg aagggccgtt acggggaggt ggacgcctcc
      601 gccatcacct tcaatgccac ttttcatttg cccgtccttg cgggcggcga gccagccaat
      661 ccggccgcct tcgaggacgt caacgatcat aaattcatct tccagcacaa cgtggactcg
      721 gacaacaacc aggaagcgct ggagctctcg gtgaaggccg actacgagtt cgagaacggc
      781 tacacgctca cgggctggat gctgtacagc gacattcaga acgacttcat cgcggacggt
      841 acgtcggcgg cgtttgggtt cttccaaggc aatgacgcct gtctggattc cacagcgacc
      901 ctgaatgccc aaggcgtgac cctgccacca ccgcagattc tcggtgcgag ccccgtgggc
      961 atcatcttta cgccggattt ctcagggtcc ttcttcgggc cctatacgcc tacaacatgc
     1021 gatggcatcc aggagcaggt gcgcgatcag gatgatttca gcttcgagct gcgtctcgca
     1081 tcgcccgtcg atcaggcgct gcgctggatg ggcggcgtct acttcctgac catcgaccgt
     1141 caggtgggga tttccctgaa cacggacagc ggcgagaagc cgatccgcgg cctgctgcag
     1201 accagcggcc cgaaccggac cgaggccctg gtttacgacg acttcgaaag cgacgtcttc
     1261 gccgtcttcg gccagctcga gtacgacatc accgatgccg tggagctctc cttcgccctg
     1321 cgctacgacc gtgaggagcg cgatgtttcc aacctcgtac ccacggatgt gtattccacc
     1381 gtgatcgatt tggtcccggg tgacgaagac ccgctcaatc ccgggctgat tggtggcgcg
     1441 attcccgaca agagcaagac gttcgaggag atccagccca aggtggcgct gacctgggac
     1501 gtgaccgagg acttcacgat cttcggcacc tggggcaagg gcttcaaggc cggcgggttc
     1561 aacaaccagg gcagtcaggc caccgtcgac ggcttcatca acgggctgat caacggagac
     1621 gtgctcggag ggcctgcatt tgccgacatt cttggcgtgc cgttgccgca gatcaacgat
     1681 gatttcgagg aggagacctc cagcgccttc gaggtgggct tcaagtccca gctgtgggat
     1741 ggccgattga gcctcgaggg cgcggcctat tacgtcgagg ttgacgacat gcagttcttc
     1801 gaattcttcg tgggtacctt cgggctcctg cgggtggtct caaacctcga cgaggtggag
     1861 atctacggat tcgagctcgc cgccacggcc cagctgcacg actacgtcag cgtctacgcg
     1921 ggtgcgaaca tcatagagag cgagatcaag gacaactcgg cgcggcccga caccgttggg
     1981 aacgacgcgc cgtacacacc cgactacacc ttcaacgtcg gagcggagct gaattacccg
     2041 ttgagcggcg acctgaatct ctttgcccgg gtggacgccc agttcgtggg tgatacctgg
     2101 ttccacacgg ttcagagcgg tcagcgtccg acgattttct ccccgctatt cgagatatcg
     2161 attttcgggg aagggtcggg gcctctcggc ttcgcggact tcgagaattc caagcgcgac
     2221 gcctacgtga cggtggacgt ccgtgccggc attcagggcg agaactggtc ggttctgggt
     2281 ttcgccaaga acatcggcga cgagaagtat ctggaggaag tgatcccggc gccggagttc
     2341 ggcggcgcgt tcgatcatcc gggggccagg cgccgttacg gggtcgaggt gagctaccgc
     2401 ttctagggta gaacctcggg gagggctgga tcgaagatgt cgaagcctct cgagggcatt
     2461 cgcctgctgg acctgacaca catgctgtcg ggtccatatg caggcatgat ccttgccgat
     2521 ctcggctgcg acaccgtgaa ggtggagccg ccgggtaccg gcgagggcac ccggcgcctg
     2581 ctcgaaaccg atcccaagaa ctcgttcaaa gggatgggcg cgtacttctt cacgctcaac
     2641 cgcaacaagc gaagcgtggc catcgacctc aaatcggaaa cgggtcgccg gtacttctac
     2701 gagctggtca aggtcgctga catcgttctg gacaacttct ccgccgatgt caccaagcga
     2761 ctgctcattg atcatgacag cctggcggcg gtgaatcccc gcatcgtgac ctgctcggtt
     2821 tccgggttcg gcgaagacgg cccccactac cggcggccgg ctttcgatca gatcgtccag
     2881 gggctcggcg ggggcatgtc catcactggc agcggccccg gtgaggcggt ccgtgccggc
     2941 atccccatcg gcgacctcgg cggtgggatg ttcgcggtca tgggcgtgca ggctgcgctt
     3001 ctggcgcgag agcgcacggg cgttggccag cacgtggaca tctccatgct cgatgtgcag
     3061 atcagcctgc tcaactacat ggcgaccatg tacaccctgt ccggcgaggt gcccgggccc
     3121 atcggcaaca gccacttcgt gcacaccccg tacaacacct tcgaaaccgc ggacgacacg
     3181 atcatcatcg cggtgatcgg tgacaccttc tggccggccc tgtgcgacct gctggagatc
     3241 gatgagctgc gcaagcctga gttcgccacg gcgcccaagc ggctcgagca caaggcgtac
     3301 atcgagggtc tcatcggtga aaggctcctt acccgtccgg cggcggagtg gctggaagcc
     3361 ctggaagcgg cgcgcatccc ctgcgcgcgg gtaaacaaca tcgcgcaggc cctggatgac
     3421 ccccagatcc tccatcggca catggtcgtg gaggtggagc atccggatgg cgggcgcgtc
     3481 aaagtgcccg gcaatcccat caagctctcc ggcacgccgg cggagacgtt ctcgccgcca
     3541 ccgctgctgg gagcccacac ccgggaagtc tttgccgagt gggcggggat ggccgaagcc
     3601 cggatcgccg aaggaatgga agccggcgcg atttcctgac ccgcgcagcg ggctggacga
     3661 ggattggaaa gatgaccgaa accgtaatca tcaacgacgt cgggccccgg gacggtctgc
     3721 agaacgacgc tgcggacgta acgcccgagg accgagcgtt gctcatcaac cgactgatcg
     3781 acgccggcgt gcccgcggtg gaggtggcga gcttcgtcag cccgcgggcc gtgccgaaga
     3841 tggccggcgc ggatcgggtc gttgccggca ttgatctctc tcgcgccgat tgcagcgcgc
     3901 tggtgcctaa tcacaagggc tacgagctgg cccgtgacgc cgggatccgc tccgttgccg
     3961 tggtgctctc ggccaccgag accatgaatc tgaagaacat caacatggat ctggagacca
     4021 cggtgcgcgt ctgcgaggag gtggtcggcc gggctgttca ggacggtatc cacgctcgtg
     4081 cttacgtatc ggtggcagtg gagtgccctt acgagggtct cgtctcagaa gaaacagtag
     4141 cgtcccttgc cgggcgcatg ttcgacgcgg gggcggccga agtgatcgtg gcggacacca
     4201 tcggcgcggc gaatccggcc caggtgaagt ctctttttga aaagctcatc ggcgccttcg
     4261 gtgctgaacg gctctcggcc cactttcacg acacccgagc gctggcattg gcaaacgcct
     4321 ggcaggcgct ggaatgcggg atccgcaagt tcgattccag cataggcggg ctggggggct
     4381 gccctttcgc gccgggcgct gctggcaatc tggccacgga agatctggcc tccatgctcg
     4441 agcaggccgg cttccatacg ggaatcgaca tggactcgtt gctctcggtg gtcgcgctgg
     4501 cgcgcgagct ggtgggtcat gacctgggtg gccgctcgct tgcctggcag ctgggcagaa
     4561 agacggaggc ggcctgacca tgacatacaa gctgggcgta gatgtcggtg gaacgttcac
     4621 ggacgtgttg ctgatcgatt ccgagagcgg cgccgtgcat acggccaagg ttccttcgac
     4681 gccggaggac tcatctgtgg gcgtgctcaa cggcattgcc agggcgtgcg ccaacgcggg
     4741 cgtggatccg gcggcgatca cccaggtcac ccacggcacc accgtggcaa ccaatgcggt
     4801 cctcaccggc cgtggcgccc tggtcggtct cgtgaccacg cgcgggtacc ggcaggtcct
     4861 gcagattgcc cgatcctacg tgcccggtgg cttgggcggc tgggtgatct acaacaagtc
     4921 ggatcccatg gcgccgctgg agtgcacggt ggaggtcccc gagcgcatcg gcgctgacgg
     4981 ctcggtagtc gaagcgctcg acgaagacgc tgccagatcg gcgcttcgtt ccctgggcgc
     5041 ccgcggcatc gaggcattga ccgtatcgct catcaacagc tttaccaacc cggcccacga
     5101 ggagcgccta gcggagatcg ccgcggaaga gctccccggc gtgcctgttt ctctgtcatc
     5161 ggaagtcatt ccggagatgc aggagtacga acgcaccatc acgaccgtgg ctaactccta
     5221 cgtcaggccc gaagtgtcgc gttacatcga caatctgcag cgggaactcg acgagcgcat
     5281 gaactcgccg aagctatcga ttctgagatc ggacggtggc ctctccacgg ctccggctgc
     5341 ggccaaatac ccggtgaacc tgctcatgag cgggccggca ggtggggttg ccggcgctct
     5401 ttgggtcggc cggcaggcgg gtttcgacaa cctcctgacc ttcgacatgg gcggcacgtc
     5461 cacggacgtt tcgctcatcg agcagggcaa agccgtggtg cgcagggaaa ccaccgttgg
     5521 tgacgtcaac gtccgggcaa gctccatgga cgtgcgcaca gtgggtgccg gcggcgggtc
     5581 catcgcctac gtgccggagc tgaccggcgc gctgcgcgta ggtccggatt cggccggagc
     5641 ggttcccggg ccggccgcct atgatctggg cggcgagcgc ccgacggtta cggatgccaa
     5701 cgtggttctc ggctacctgc ccgcggagca gaagcttggc ggcgacatga ccatcagccg
     5761 ggacaaagcg gcggccgccg tgcagcagat cgctgacgcc atggggctct ccctcgaagc
     5821 cgcg
//
LOCUS       DBNHOX010000534         6995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1714586, whole genome shotgun sequence.
ACCESSION   DBNHOX010000534 DBNHOX010000000
VERSION     DBNHOX010000534.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1714586"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="AC16UZ_11720"
     CDS             complement(<1..268)
                     /locus_tag="AC16UZ_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR6247395.1"
                     /translation="MRKFLGRMTRRGIVSLLAGLLVTLPIGVNAAKDQSPVLAQIAKW
                     GKIRVGMSGSQPPYNFVSREGKLIGMDVDIAELLAKSLDVQLEMV"
     gene            complement(389..1405)
                     /locus_tag="AC16UZ_11725"
     CDS             complement(389..1405)
                     /locus_tag="AC16UZ_11725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HIR6247396.1"
                     /translation="MQAIRCHEISEDIGAVRLETISLDPPGEGEVQVRLKACAVNFPD
                     LLMIQGKYQFKPPLPFSPGTEAAGDVVAVGDGVSAFDVGDRVVVALRYGGFAEAVNVP
                     AESVQPVPGSMSYDKAASYRTAYLTAYVALAKRGDLTPGEVLLVHGATGGVGMAAVDL
                     GKRLGAVVIATGGSDEKLAVVKARGADHVINYTQADGTLGGFREEVKALTDGRGADVI
                     YDPVGGDVFDESMRCINWGGRILTIGFTSGRWPQAPVNLILIKQIAVIGVRAGEYGRR
                     DPAKGREAERALYAMAAAGEIDPYVCAGFPLERAVEAMRMLEQRKVVGKVVVTMNGFD
                     VASP"
     gene            complement(1415..1810)
                     /locus_tag="AC16UZ_11730"
     CDS             complement(1415..1810)
                     /locus_tag="AC16UZ_11730"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6247397.1"
                     /translation="MIRDAETFEHLLAAVRRFVAEVAVPNEERVEADDEIPAEGVEAM
                     RNLGTFGRSIPEAYGGSGLTSEELALAFMELTRCSVAYRVVQIFGGQGYVKGRGSERF
                     YRDVRAFRIYEGTSQIHLLNIAKQLLKDA"
     gene            complement(1810..2754)
                     /locus_tag="AC16UZ_11735"
     CDS             complement(1810..2754)
                     /locus_tag="AC16UZ_11735"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6247398.1"
                     /translation="MEIYLHHYPASLFSEKIRLLLGYLGGSWHSVEIPSIMPRPLLMP
                     LSGGYRRTPVLQIGANVYCDTAVIARGLARHFGDEDLFAAGFTANRVAEWADTQLFRV
                     TVALNFRPEAVGGFMSQLSPEEIAAFQKDRAELVGDAPMVSFSPEAAQAYLATYLDQA
                     DRALDGGFLFGGKPCIADFCFYHCLWFLENNPLNAPLLDAYGNVRGWMQTMAGFGHGS
                     VAPSSGEAALEHARSCSPVAPVLESPLAAPFELDVGRRVTVTPADYGRVPVAGSLVAW
                     DCEEVVILREDAATGPIMNHFPMAGFQVAPAGNLGSSG"
     gene            complement(2839..4164)
                     /locus_tag="AC16UZ_11740"
     CDS             complement(2839..4164)
                     /locus_tag="AC16UZ_11740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR6247399.1"
                     /translation="MMRDTSLTGLAESLVAYGTRRGADQVEVAIRAGSNFSASIREGE
                     LETLVEAGFRELEVKLIVDQKVATEASSDLSEATLKRLIDNGIGRARLSGADPFAGLP
                     EKEPVKVRAEALDIFDEAVVDMSPESKIAAARRVEAICLGDKRIRRSMGAGFGTTIET
                     LYLANSNGFSDAFRQTACSNGVYLQVGEPDNLISEGKYDYSRNLEGLRSPEDIAAEAI
                     DRSTRLIGARKVPTQNVPVVLEPEMSAELLQFLASCVDGRGVYRKQSFLADRIGERVA
                     GANVNVFDDGLMPGAPGAKPFDREGVPLRKTAVIEKGVLKSFLLDSYSARKLGMKPTG
                     NGAGPNNLYLASGKDSPADIIGSVDEGLLLTGSIGFGAVPTTGDISRGAFGIWIEGGE
                     LAYPVAGITIGANLGTLLQQVEMIGDDLDHRRYVNGPTIKVAEMTIGGT"
     gene            complement(4161..5684)
                     /locus_tag="AC16UZ_11745"
     CDS             complement(4161..5684)
                     /locus_tag="AC16UZ_11745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR6247400.1"
                     /translation="MRADRRGFLKKTGSAVAAASTASLWPDLLLAEEVPTTAEALFET
                     RFGVSRGDMGRLLSLALGKGGEFAELFFEYKIANGVTMEEDIVKESSERVSLGVGIRV
                     LKGEQTGYGYTSDLTFEGMSQAARTAAAIASEGGRVEIAPLRLRANALQVYDLERPFA
                     ESSLSSRMILLRQTYDAAQAYHPKIEKVRVSLRDEIQYVLVANSEGLLASDTRPQVRL
                     IASVTAADGGNRSTGLSTAGGRVGSAFFSSARTPKELGREAAEEAIVLLGAVSPRPGD
                     QTVVLDSGQSGVMIHEAVGHPLEADSNRKKRSVFWDKLNQTVANPLVTIYDDPTIAHF
                     RGSHNMDDEGTLPRKTMLIEKGKLVGYLQDRLSAKLMKMSPSGHGRRDGFENVPLPRM
                     ANTNLARGESDPEQIVRSVKRGFFAKTYQGGMVQPSGKFTFSVNMGYLIEDGKLTAPV
                     KNATLIGSILDVLPEVTMVGNDMGTFLGTCGKGGQSAPVTAGTPTLKINSMTVGGRS"
     gene            complement(5753..>6995)
                     /locus_tag="AC16UZ_11750"
     CDS             complement(5753..>6995)
                     /locus_tag="AC16UZ_11750"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR6247401.1"
                     /translation="YHQRFGKVNPKTLVSTWNFGRQLAPGVDAADTMNQIVESGSLRE
                     GLAARVPRGPLYRNIQMALARYRSAAAAGGWEPVPEGVTLTIGMRDERVVSVRRRLAV
                     TGDLETGAVSDPELFDADLESGVRRFQHRHSLSVDGAVGPATVRAMNVSVEERIDQLR
                     LSLERARWIMTARDTSVVVVNIAAAEVYVLEEGEMVWQRRAMVGRTYRQTPVFRGLMT
                     YLEINPTWTVPPGILRADILPKVREDPGYLARSNMTLLDRSGRAVDPYAVDWQALEGM
                     PYIFRQEPGPTNALGLIKFMFPNEHFVFIHDTPNRELFERAERTFSSGCIRVEDPFSL
                     AALLLRDPANWDEAAVRAAVETGETRRVRLADPWPVYILYWTAQVDDDGVVRFYPDVY
                     QRDVELLGELDGDVRIDLPAS"
BASE COUNT         1253 a         2399 c         2119 g         1224 t
ORIGIN      
        1 gcaccatctc gagctgaaca tcgagcgact tggccagcag ctcggcgatg tccacgtcca
       61 tgccgatgag cttgccctcc cggctgacga agttgtacgg cggctggcta ccggacatgc
      121 cgacgcggat cttgccccac ttggcgatct gagcgagaac gggcgactga tccttggcgg
      181 cattgacgcc gatggggaga gtgaccagga ggccggcgag cagactgaca atgccccttc
      241 tggtcatacg tccgagaaac ttacgcatgg gtcttcctta gttgattggt ttccatattt
      301 cttgacgccc gccttcaaac gggcgggtcg aacggggagg ccgttataca acaagccagg
      361 cgcgacgaac agcctctcgg cggaggagct atggtgaggc gacatcaaag ccgttcatgg
      421 tgaccaccac cttgccaacc accttgcgct gctcgagcat tcgcatggcc tctaccgccc
      481 gctccagcgg aaaacccgcg cagacgtacg ggtcgatctc gccggcggcc gccatggcat
      541 acagtgcccg ctcggcctcg cgccccttcg cgggatcccg ccgcccgtac tcgccggcgc
      601 gaacgccgat cacggcaatc tgcttgatca gaatcagatt aacgggcgcc tggggccagc
      661 gcccgctggt gaacccgatg gtaaggatgc ggccgcccca attgatgcag cgcatggatt
      721 catcgaacac gtcgccgcca acgggatcgt agatgacgtc agccccccgc ccgtcggtca
      781 gcgcctttac ttcctcgcga aacccgccca gcgtcccatc ggcctgggtg tagttgatca
      841 catgatccgc gccacgggcc ttgaccaccg ccagcttctc gtcgctcccg ccagttgcga
      901 tgaccacggc acccagacgt ttgcccagat ccaccgccgc catgcccaca cctccggtgg
      961 caccgtgaac gagcagcacc tcgccgggcg tcaggtcgcc acgcttggcc agcgccacgt
     1021 aggcggtgag gtaggccgtc cggtacgaag ccgccttgtc gtagctcatc gagccgggaa
     1081 cgggctgcac actctccgcg ggcacattca ccgcctccgc gaaaccgccg tagcgcagcg
     1141 ccacgaccac ccggtcgccc acgtcgaacg cgctcacccc atcgccgacg gcgacgacgt
     1201 caccggcggc ttccgtgccg ggagaaaacg gcaggggcgg cttgaactga tactttccct
     1261 ggatcatgag cagatcggga aagttgaccg cacaggcctt caggcgcacc tgaacctccc
     1321 cctcacccgg cgggtcgagg ctgatggttt cgagacggac agcgccgatg tcctcggaga
     1381 tctcgtgaca gcggatggct tgaatatcgg tcacctaggc gtccttgagc agttgcttgg
     1441 caatgttgag cagatgaatc tggctggtac cctcgtagat ccgaaacgcg cgaacgtccc
     1501 gatagaaccg ctcgctgccg cggcccttga cgtagccctg cccaccgaag atctgcacca
     1561 cccggtaggc taccgagcaa cgggtcagct ccatgaaggc gagcgcaagc tcctcgctgg
     1621 tgagaccgct gccgccatag gcctcaggga tgctccggcc gaaggtaccg agattacgca
     1681 tcgcctccac gccctccgcc gggatctcat cgtccgcctc cacacgctcc tcgttgggaa
     1741 cggcaacctc cgcgacgaat cggcgcacgg ctgccagcag atgctcgaag gtttctgcgt
     1801 ccctgatcat cagcccgacg accccagatt gccggcggga gccacctgaa agcctgccat
     1861 cggaaagtgg ttcatgatcg gcccggtagc cgcgtcctct cgcaagatca cgacctcctc
     1921 gcaatcccag gcaacgaggg atccggccac gggcactcgt ccgtagtcgg ccggcgtcac
     1981 ggtgacacgc cgacccacat cgagctcgaa gggagccgca agcgggctct ccagtaccgg
     2041 ggcgacggga ctgcaggacc gcgcgtgctc gagcgcagcc tcgccgctgc ttggtgcaac
     2101 ggatccgtga ccgaagcccg ccatggtctg catccagccg cgaacgttcc cataggcgtc
     2161 cagcagagga gcgttcagcg gattgttttc cagaaaccac agacaatgat aaaagcagaa
     2221 gtcggcgatg cagggcttcc cgccgaacag gaacccgccg tcgagcgccc ggtctgcctg
     2281 gtcgaggtag gtggccagat aggcctgcgc cgcttcaggg gagaacgaga ccatcggcgc
     2341 atcacccacg agctcggccc ggtccttctg aaaggctgca atctcttccg gggaaagctg
     2401 gctcatgaaa ccgccgaccg cctccggccg gaagttgagc gcgaccgtca cgcggaacag
     2461 ttgggtatcc gcccactccg cgacccggtt ggccgtaaag ccggccgcaa acagatcttc
     2521 gtcgccgaaa tgccgggcga gccccctggc aatgaccgcg gtatcgcagt agacgttggc
     2581 accgatctgc agcacgggcg tgcgtcgata gcctcccgac agcggcatca gcaaagggcg
     2641 cggcatgatg ctcggtatct cgactgagtg ccaggaaccg cccaggtacc ccagaagcag
     2701 ccggatcttc tccgaaaaca gggaagccgg atagtggtgc aggtaaattt ccaactgttc
     2761 ccccagaacg gaccttcgcg gtacgcgcgc gaccactgcg tttgccgccc gggacacacg
     2821 cgtgctccgt acccggcctc aggtcccgcc gatggtcatc tccgccacct tgatggtcgg
     2881 gccgttgacg tagcggcgat ggtccaggtc gtcgccgatc atctccacct gctgcagcag
     2941 cgtcccaaga ttagcaccga tggtgattcc ggcaaccggg taagcgagtt cgcccccttc
     3001 gatccagatg ccgaaggcgc cccgggagat gtcgcccgtg gtgggcaccg ccccgaaccc
     3061 gatgctgccc gtgaggagca ggccctcgtc cactgaaccg atgatgtccg caggagaatc
     3121 ctttcctgac gctagataga gattgttcgg ccccgccccg ttccccgtgg gcttcatccc
     3181 cagctttcga gcggagtagg agtcgagcag gaagctcttg agcacccctt tttcaatcac
     3241 cgccgtcttg cgcaggggga ccccctcgcg gtcgaagggc ttcgcgcccg gcgcgcccgg
     3301 catcagaccg tcgtcgaata cgttcacgtt cgccccagcc acccgctcgc cgatccggtc
     3361 ggccagaaag ctctgcttgc gatacacacc gcggccatcg acgcagctcg cgagaaactg
     3421 caacagctct gcgctcatct ccggctccag gaccacgggc acgttctgag taggaacctt
     3481 gcgggcgcca atcaggcggg tagatcgatc gatggcctcc gccgcgatgt cctccgggct
     3541 cctcagccct tcgaggttac gggagtagtc gtactttccc tcgctgatga ggttgtccgg
     3601 ctcccccacc tggaggtaca cgccattgga acaggccgtc tgccggaacg cgtcgctgaa
     3661 cccgttggaa ttcgcgagat acagcgtctc tatggtggtg ccgaagccgg cacccatgga
     3721 tcgcctgatt cgtttgtcgc caaggcaaat ggcctccacg cggcgtgcag ccgcgatctt
     3781 gctctccgga gacatgtcga ccaccgcctc gtcgaatatg tcgagggctt cggctcgtac
     3841 cttgaccggc tccttctccg gcaaccctgc aaacgggtcc gcgccggaca accgcgcccg
     3901 tccgatcccg ttgtcgatca gccgcttcaa cgtcgcctcc gacagatcgg atgacgcctc
     3961 cgtggcgacc ttctggtcga cgatcagctt cacctcgagc tcccggaaac ctgcctccac
     4021 cagggtttcc agctcgcctt ccctgatgga cgcagagaag ttgctgcccg cgcggatggc
     4081 aacctcgacc tgatccgcac cacgccgggt tccgtaagca accaacgact cggcgagccc
     4141 cgtcaggctg gtgtcgcgca tcatgagcgg ccacccacgg tcatgctgtt gatcttcaga
     4201 gtgggcgtgc ccgccgtcac gggcgcgctc tgaccgccct tgccgcatgt tcccaggaac
     4261 gtgcccatgt cgttgcccac catggtcacc tcgggaagca cgtccagaat gctgccgatc
     4321 agcgtggcgt tcttgaccgg cgcggtgagc ttgccgtcct cgatgaggta acccatgttg
     4381 accgagaagg tgaacttgcc cgaaggctgc accatgccgc cctggtaggt cttggcgaag
     4441 aacccgcgtt tgacggaccg gacgatttgt tccgggtcgg actctccgcg cgcgaggttc
     4501 gtgttggcca ttcgcggcag cgggacgttc tcgaaaccat cacgacggcc gtggccgctc
     4561 ggcgacatct tcatgagctt tgccgagaga cggtcctgca ggtagccgac cagcttgccc
     4621 ttctcgatca gcatggtctt tcggggcagc gtgccttcgt cgtccatatt gtggctgccc
     4681 ctgaagtgcg caatggtggg atcgtcgtag atggtgacca ggggattggc gacggtctgg
     4741 ttcagcttgt cccagaacac cgaccgcttt ttgcggttcg agtccgcttc cagcgggtgg
     4801 ccgaccgcct cgtgaatcat gacgccggat tgaccggaat cgagcaccac cgtctggtcc
     4861 cccggtcggg ggctgaccgc accgagcaac acgatggcct cctcggccgc ctcccgaccg
     4921 agctccttcg gcgtgcgcgc cgaactgaaa aaagcggatc ccactcgccc accggccgtc
     4981 gacagtcccg tgctgcgatt gcctccgtcg gccgcggtga ccgaggcaat gagtcggacc
     5041 tgcggccgcg tatccgatgc cagcaggccc tcgctgttgg caaccaggac gtactgaatc
     5101 tcgtctctca gagacacccg caccttctca atcttcgggt ggtaggcctg agcggcatcg
     5161 tacgtctgcc taaggaggat cattcggctg ctcagggaac tctctgcaaa cggtcgttcg
     5221 aggtcgtaga cctgcaaggc gttggcgcgg agcctcaacg gtgcaatctc caccctgccc
     5281 ccttcagagg cgatcgccgc ggccgtgcgc gctgcctgac tcatgccctc gaaggtgagg
     5341 tcgctcgtgt acccgtagcc ggtctgctct cccttgagca cccggatgcc gacaccgagg
     5401 ctgacccgct ccgaggactc cttgacgatg tcctcctcca tggtgacccc gttggcgatc
     5461 ttgtactcga agaacagctc ggcaaactcg ccccccttgc cgagcgccag cgacaggagc
     5521 ctgcccatgt cgccacggct cacgccgaag cgcgtttcga acagggcttc tgccgtggtg
     5581 ggcacctcct ctgcgagcag caggtcgggc caaagggacg ccgtggaggc ggccgcgacc
     5641 gcagagcccg ttttcttcag aaagccgcgt ctgtctgccc gcattgtgac gagttctcct
     5701 ttttcactga tgtagcgccc tcagtcggca gcatcgctcg aaccagccgg gttcaggagg
     5761 cgggaagatc gatccgaacg tcgccgtcga gttccccaag cagctccacg tcgcgctgat
     5821 acacatccgg gtagaagcgg acgactccgt cgtcgtccac ctgggcggtc cagtagagga
     5881 tatagacggg ccacggatcc gcaaggcgga cccgccgggt ctcaccggtt tccaccgcgg
     5941 cgcgcaccgc ggcttcgtcc cagttcgccg gatcgcgaag aagcagggcg gcgagggaaa
     6001 acggatcctc caccctgatg cagcccgagc tgaacgtacg ctccgcccgt tcgaagagct
     6061 cacggttcgg ggtgtcgtgg atgaagacga agtgctcgtt gggaaacatg aacttgatca
     6121 ggcccagcgc gttcgtcggg cccggctcct gccggaagat gtaaggcatg ccctcgagag
     6181 cctgccagtc cacggcgtag gggtcgacgg cgcgacccga tcggtccagc agcgtcatgt
     6241 tggagcgggc cagatatcca ggatcctcgc gcaccttcgg caggatgtcc gcccgcagaa
     6301 ttccgggcgg taccgtccag gtggggttga tttccagata ggtcataagg ccgcgaaaca
     6361 ccggcgtctg gcgataggtc cggccgacca tggcccgacg ctgccagacc atctcacctt
     6421 cttccagaac gtagacctcc gcggccgcga tgttcacgac gaccaccgac gtgtcccgcg
     6481 cggtcatgat ccatcgcgcc ctttcaaggg acaggcgcag ctggtcgatc cgctcctcca
     6541 cggagacgtt catggcgcgc accgtagcgg gaccaacggc cccgtccacc gacaggctgt
     6601 ggcgatgctg gaaccgcctg acaccgcttt ccaggtcagc gtcgaacagt tccgggtcgg
     6661 acactgcgcc cgtctcgaga tcgcccgtta cggccagccg ccggcgaacg ctgacaaccc
     6721 gctcgtcgcg catgccgatg gtcaatgtga cgccctcggg gacgggctcc catccccccg
     6781 ccgcggcggc ggaccggtac ctcgcgagcg ccatctggat gttacggtag agggggccac
     6841 gcgggacgcg agcagccagc ccttcgcgaa gactccccga ctccacgatc tggttcatgg
     6901 tgtccgcagc atcgacgccc ggcgcgagct gccggccaaa gttccacgtg ctcacgagcg
     6961 tcttcggatt gaccttgccg aaccgctggt gatag
//
LOCUS       DBNHOX010000535         3901 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1393274, whole genome shotgun sequence.
ACCESSION   DBNHOX010000535 DBNHOX010000000
VERSION     DBNHOX010000535.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1393274"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="AC16UZ_11755"
     CDS             complement(<1..695)
                     /locus_tag="AC16UZ_11755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6247402.1"
                     /translation="MATPRPTQAEAFLKKFHDRRPGLTPQAFSSLPVHLDGMQYPSTY
                     HLLASTVAHTADGCEVLDLACGDGYLLSLIASAADSRAALYGIDMSFGELAAAATRLG
                     SRATLTSGRAQELPYPAGQFDAVLCHMALMLMEDLDLVLTEVRRTMRPKGTFAGVVGA
                     PSPPSPALTKFIDVFSRCSRRPEWEGLRLGDPRLRRKDGLIHVLSPKFEVSIIQDLPL
                     ALRLDPESLWLWLL"
     gene            complement(1133..3055)
                     /locus_tag="AC16UZ_11760"
     CDS             complement(1133..3055)
                     /locus_tag="AC16UZ_11760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR6247403.1"
                     /translation="MLIPPVMPRAGKRVRAGGKNADYYEISVKQFPQQILPVGSPATT
                     VWGYGAVASENMRGLLLHNAPSLTIEAEYDRPTLIKWINDLVDANGKYRPHLLPVDQT
                     LHWANPPGGSTGRDTRPTFQSTPDYYTGPVPIVTHVHGAVGVGDESDGYAEAWYLPAA
                     NDIPADYAAEGTWYDFFAGKAMAKFGEAWGPGYAVCQYPNLGRPAADWYHDHTLGMTR
                     LNVYAGPAGFYIIRGGPAGDDAVFDTRFGTKAVLPGPAPKANDKFPPNKTYYEIPIAI
                     QDRSFNSDGTLFYPDTRAFFDGITGPFIPDTDISPSWNPEFFGNMMMVNGNTWPFQVV
                     EQRRYRLRLLNGCQSRFLILDFDHIPGVEVWQIGNEGGFLAAPVNLTADNGNRLLMAP
                     AERADVIVDFTDTHVGDYRLANVGPDEPFGGGVPGVDFPLADADTTGQVMEFRVVPAV
                     RSDDSTPPEFLVLPAITQLPTATVTRPLALLEEVSSFFEAPAETLLGTVTGDPNTTEP
                     GVWVKRLWMEPVTENPALGSTEVWEFYNATADAHPIHIHEVLFEVLNRQAISVDEMQQ
                     EVRVIPGSVPTPPEPWETGFKDTVIAYPGQVTRIRAQFDTPGQFVWHCHIVEHEDNEM
                     MRPYRIGPVQPGQPGL"
     gene            complement(3685..>3901)
                     /locus_tag="AC16UZ_11765"
     CDS             complement(3685..>3901)
                     /locus_tag="AC16UZ_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247404.1"
                     /translation="DGDPVAYFAENDYKFRLLLNADDVAKGYGMFGTPRILVVDGGKV
                     VRYTIKKGTSLEQLLTDVREALERSSS"
BASE COUNT          736 a         1175 c         1240 g          750 t
ORIGIN      
        1 agcagccaaa gccagaggct ctcggggtcg agtcgtaggg caagtggcag atcttggatt
       61 atggagactt caaactttgg cgaaagaaca tggatcaatc cgtccttccg gcgcaggcgc
      121 ggatcaccaa gccgaagacc ctcccactcg gggcgccgag agcagcggga gaagacatcg
      181 atgaatttcg ttaacgccgg tgacggcggc gacggcgccc caaccactcc agcgaaggtt
      241 cccttggggc gcattgttcg gcgtacctcg gtgagcacca agtccaggtc ttccatgagc
      301 ataagtgcca tgtggcaaag cactgcgtcg aattggccag cggggtatgg aagttcctgg
      361 gcacgaccgg aggtgagcgt ggcccgcgaa ccaaggcggg tggcggcagc ggccagctca
      421 ccgaagctca tgtcaatccc gtaaagcgcg gctcgactat cagcagcaga tgcaatgaga
      481 gacagcaggt acccgtcgcc gcaagcgagg tctagtacct cacatccatc ggcggtgtgg
      541 gcaacagtgc ttgcgagtag gtggtaggta gacgggtact gcattccatc tagatgcact
      601 ggaagggacg agaacgcctg gggagtcagc cctggccggc gatcatgaaa cttcttgaga
      661 aatgcttcag cctgcgtggg acgaggcgtt gccataggcg gcctaactac taattgaatg
      721 gcccagttgc gccgaatcta tcacgcatct gtgtccgtat agcagccccg cccagcgcaa
      781 gccggactgg cagcgttgcg tcgttagcag accttcacgg ccagtgaggg ccgacccaga
      841 cgggacgtcc gctttcggcc agtaacagac atgggtggga cataggaacc ccggcgctgg
      901 gccgggggtt tcttgaaagg ttgttagcgg aggcgcgcta ctttgatggc aggaatatac
      961 tgggcatcgg gagatgatcg aggccgaact gcggtagcgc gacacgcccc cgtctcctcg
     1021 gcatccactg ctgaaaagat atcgcccgtc tttctatctt tgattctcac cccgccgcta
     1081 tcagcagatg ccgggctcag ggcccgtctg cttgctacac gcgtcgaacg actcacagtc
     1141 ctggctggcc gggctgtacc ggtccgatcc ggtatggccg catcatctcg ttgtcctcgt
     1201 gctcgacgat gtggcagtgc catacgaact gaccgggcgt gtcgaattgc gcccggatgc
     1261 gcgtgacctg ccccggatag gctatgaccg tgtccttgaa gccggtctcc cacggttcgg
     1321 gcggtgtcgg cacggaccct ggaatgaccc ggacttcctg ctgcatttca tcgaccgaaa
     1381 tggcctgtcg attcagaacc tcgaacaaca cctcgtggat gtggattgga tgggcgtcag
     1441 ccgttgcgtt gtagaattcc cagacttcgg tcgagccgag ggcgggattc tccgtcacag
     1501 gctccatcca gagccgcttt acccagacac cgggctcggt ggtgttcggg tccccagtca
     1561 cggtgcccag caatgtctcg gcaggcgcct cgaagaagga ggaaacctcc tccagcaaag
     1621 ccagcggccg agtcacggtc gccgttggga gctgggtgat cgccggcaac accaggaact
     1681 ccggcggcgt cgaatcgtcg gacctgactg caggcacaac tctgaactcc atgacctggc
     1741 ccgtcgtatc cgcatcggcg agcgggaagt cgacaccggg cacgcccccg ccgaatggct
     1801 cgtcgggccc gacattcgcg agacggtagt cgccgacgtg cgtgtccgtg aagtcgacga
     1861 ttacgtcggc acgttcggcg ggtgccatca gcaggcgatt cccgttatcg gccgtgagat
     1921 tcactggcgc cgccaggaat cctccctcgt tgccgatctg ccagacctcg acgccgggga
     1981 tgtggtcgaa gtcgaggatc aggaaccgcg actggcagcc gttcagcaat cgcaggcgat
     2041 agcggcgctg ctccacgacc tggaatggcc aggtattgcc gttcaccatc atcatgttgc
     2101 cgaagaactc ggggttccat gacggcgaga tgtctgtgtc cgggatgaag ggccccgtga
     2161 ttccgtcgaa gaacgcgcgg gtgtccggat agaacagcgt cccgtcgcta ttaaacgagc
     2221 gatcctgaat cgcgatcggg atctcgtaat aggtcttgtt cggcgggaac ttgtcgttgg
     2281 ccttgggcgc cgggccgggc aggactgcct tcgtgccgaa gcgcgtgtcg aacacggcgt
     2341 cgtcaccggc cggaccgccg cggatgatgt agaagcccgc cggaccggca tagacgttga
     2401 ggcgcgtcat gccgagcgtg tggtcgtggt accagtcggc ggcaggccgg cccaggttcg
     2461 ggtactggca gacagcgtag cccggtcccc aggcttcgcc aaatttggcc atggccttgc
     2521 cggcgaagaa gtcgtaccag gtgccttcgg ccgcgtaatc ggctggaatg tcgttggccg
     2581 ccggcaagta ccaggcttcc gcgtagccgt cgctctcgtc cccgacgccc acggcgccgt
     2641 ggacgtgagt cacgatcgga acaggtccgg tgtaatagtc gggcgtgctt tggaatgtcg
     2701 ggcgtgtgtc gcgaccagtc gatccacccg gcggattggc ccaatgcaac gtctggtcta
     2761 ccggcagcag gtgcggtcgg tatttgccgt tggcgtcgac caggtcattg atccacttga
     2821 tcagggtcgg ccgatcatat tccgcctcga tcgtcagcga cggggcattg tgcagcagca
     2881 gtccgcgcat gttctcggat gcaacggccc catagcccca cacggtcgtt gccggggatc
     2941 ccacaggaag gatctgctgc gggaactgct tcactgagat ctcgtagtaa tcggcgttct
     3001 tgccgccggc cctgactcgc ttgcccgccc tcggcatgac cggcgggatc agcatcggtg
     3061 tcacgtactt ctggaccgag tgcgggtcga gagttccacc ggggatctgt gcaatggctt
     3121 gcctgagccc cagcttcgtt gaggcgtata gcgtgagcgt ggtcgcgccc gtgtacttga
     3181 tgaagcctcg gcgtgtaatc ctcagcttgc tcgtcctgga tcttcccctg cgcatggtaa
     3241 cggtcctccc tgaggctgcg gatggctgaa ccggcttcgc ggcactcgtc gcccaagcaa
     3301 tggatgtcgg agcgttgcgc ccagtggccc ccttcgatga cggttgtttc tcgttattgc
     3361 ccgccctcga cttacagccg gctagacgca gcggaaaccg cgcccgaacg gacatgattc
     3421 agactttaag aaatgaaacc gctaaaggcc ctacgtgaag accgaggctt gaactccgca
     3481 ctgaatcgct tccgtcttgc catgacacac ccccagggca ttattacgcc caatcaggtg
     3541 gtccagaaaa tcggggtcag accatccggc cagaagcgga catccgtcga aaggagaaac
     3601 cccggcgtgg gcccggggtc gacgagcact ggctctggac ccgtactggc ggggcaacga
     3661 gtccaggagg agaactgcct cgggctagct gctagatcgt tccagggcct ctctcacgtc
     3721 tgtcagcagt tgctcgagcg atgtgccctt ctttatcgtg taacggacga ccttgccgcc
     3781 gtcgacgacc agaatgcggg gcgtgccgaa catgccgtag cctttcgcga catcgtcggc
     3841 gttcagcagc aaccggaact tgtagtcgtt ctcggcaaag taggcgacgg gatcgccatc
     3901 c
//
LOCUS       DBNHOX010000536         1894 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1200297, whole genome shotgun sequence.
ACCESSION   DBNHOX010000536 DBNHOX010000000
VERSION     DBNHOX010000536.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1200297"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="AC16UZ_11770"
     CDS             complement(<1..221)
                     /locus_tag="AC16UZ_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247405.1"
                     /translation="MSATPCLPAIPAAIPARKALLGACCALLCMGLAGHAAAQITPSK
                     ESLSNLYPVKAYSPYAERRFPDRVFWGDT"
     gene            419..1648
                     /locus_tag="AC16UZ_11775"
     CDS             419..1648
                     /locus_tag="AC16UZ_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6247406.1"
                     /translation="MTQLDLDFVRGQFPAFSEPSLAGFAHFENAGGSYACGQTIDWLE
                     RYYRQTKVQPYYDFPASRTAGEQMDAARRRMAEWLNVGVEELHFGPSTSQNSYVIAQA
                     LRQELKPGDEMIVTNQDHEANVGAWARLEDAGIVVREWKIDPDTAELHPMDLEALLSE
                     RTRAVAFTHCSNIVASINPVREITDLIHEAGAWAIVDGVSFCPHGMPDIAALGADVYF
                     FSLYKVYGPHLGAMYLSRSLNAELPYQGHFFNADKPSARFTPAGPDHAQIAAVNGVMD
                     YMEAVAAHHGHGGDAAQGRAEAVRTLFREQETALLQPLLDFAAGHPKLHLIGKDHAAT
                     RAPTMAFTADGWRSADLAARLAEFGLGVGVGHFDAYRLVEALGIDTDDGVLRASFVHY
                     TRPEEVSRLIEALDTLL"
BASE COUNT          334 a          626 c          598 g          336 t
ORIGIN      
        1 tgggtgtcgc cccagaagac ccggtcggga aatctgcgtt cggcatacgg ggaataagcc
       61 ttgaccggat agaggttgga aagcgactct ttcgacgggg tgatctgcgc cgcggcatgg
      121 ccggcaagcc ccatgcacag caacgcgcag cacgcgccga gaagcgcttt ccttgcgggt
      181 atggcggctg gaatcgctgg taggcaaggc gtcgctgaca tgatgggtcc tccctctgtc
      241 cggtttgacc cgaccccaat atcgtcgggc tgagtccccc atgttacaac acgaacccgt
      301 gctgggcgtc acctggcccg gttttccggc gcggggttgc cgctttgtaa ggtcctcgat
      361 gtacggaaaa acgggccgtc ctgatatcat tcgtcgcaaa cggcaagaga catctcccat
      421 gacccaactc gacctggatt tcgttcgcgg gcaatttccg gctttcagcg agccttcgct
      481 cgcaggcttc gcgcatttcg agaatgccgg cggctcttat gcctgcgggc agaccatcga
      541 ttggctggag cggtattacc ggcagaccaa ggtccagcca tactacgatt tcccggcctc
      601 ccgaaccgcc ggggagcaga tggacgcggc caggcggcgc atggcggagt ggctgaatgt
      661 gggcgtcgag gaactgcatt tcgggccctc cacgtcgcag aacagctacg tcattgccca
      721 ggccttgcgc caggagctga aacccggtga cgagatgatc gtcaccaacc aggaccacga
      781 agccaacgtg ggcgcctggg ccaggctcga agacgcgggc atcgtcgtgc gtgagtggaa
      841 gatcgatccc gacaccgccg agctgcaccc gatggacctc gaggccctgc tcagcgagcg
      901 gacccgcgca gtcgcgttca cgcattgctc gaacatcgtc gccagcatca accccgtgcg
      961 cgagatcacc gatctgatcc acgaggccgg cgcctgggcg atcgtcgacg gcgtgtcctt
     1021 ttgcccgcac ggcatgccgg acattgccgc gctgggtgcg gacgtctact ttttctcgct
     1081 gtacaaggtc tacgggccgc acctcggcgc catgtacctg agccgcagcc tcaatgccga
     1141 gctgccttac cagggccact ttttcaatgc cgacaaacct tcggcccgct tcaccccggc
     1201 cgggccggac catgcgcaga tcgctgccgt caacggcgtc atggattaca tggaagccgt
     1261 tgccgcgcat cacgggcacg gcggggatgc ggctcagggc cgggctgaag ccgtgcggac
     1321 gctgttccgc gaacaggaga ccgccctgct ccagccgctg ctggatttcg cggccggcca
     1381 tccgaaactc cacctgatcg gcaaggacca cgccgccacc cgggcgccga cgatggcttt
     1441 caccgcagac ggctggcgtt cggctgatct cgccgcgcgc ctggcagagt tcgggctcgg
     1501 tgtcggcgtc gggcacttcg acgcctaccg gctggtggaa gccctgggca tcgacaccga
     1561 tgacggcgtc ctgcgcgcct ccttcgtcca ttacacccgc ccggaggagg tcagccgcct
     1621 gatcgaagcc ctggacacgc tgttgtagga ggccgcgcgc caaggcgttc agcaggtact
     1681 tctttccgct tcgccgaaaa ccgcccatgc ttcattgcct gcgcttgcgg gcgacccgaa
     1741 acagggtctg gaagaggcgc ccagggcccg cgttcaggac aatcgggctg tcgggaacac
     1801 acgcaacggc tgtgggtgag cgcttctttg cctccagcaa agccgcactc gcccacagag
     1861 cctgcgctcg attagcgcac agggatgtct tgag
//
LOCUS       DBNHOX010000537         3471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_236977, whole genome shotgun sequence.
ACCESSION   DBNHOX010000537 DBNHOX010000000
VERSION     DBNHOX010000537.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_236977"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3471)
                     /locus_tag="AC16UZ_11780"
     CDS             complement(<1..>3471)
                     /locus_tag="AC16UZ_11780"
                     /inference="COORDINATES: protein motif:HMM:NF037144.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HIR6247407.1"
                     /translation="EHVFAVRGNGLPFYRYNVASDSWTTLASTPANIKKGGALTTDGT
                     YLYLTRGDRKPDFYRYDIAANTWTAMASLPQNVGWGGSLARVGGLIYALQGDAKKGLY
                     RYDIASNTWSALAAAPGNVADGGALVTDGGNYLYAFQGKTKVFWRYNIATNSWTTLSS
                     VNFAANVGQGGALAYVPPLNPEGYETSIKATPSVLTTGDEIVFEMTISASQPVSNVVT
                     GSCSITGTNGATASLSTGPTLSSTDDDISGPDDLVVYRWTYQATAGSLPGSLSLTCNA
                     SGDGSTSFPSATSRTSLVSPVLTFQVTVNNPPPASGLIQNTALIGEASSALVSVSSPT
                     TQTSLAAGIGDRVWKDTDGDGLQDPGEPGISGVTVELYDGSGALLATTTTDASGYYSF
                     DGLTTGTYSVGYVYSTVPAGWIPTTDVTLAGIVLAAGQQYDDADFGLEPPPPVEDSSS
                     IGGTVWHDVDEDGTADPGEALLSGVSVNLYADLNNNGLIDSGDVLVATTTTSADGSYS
                     FSGLGTGSYLVDVDESDPDFSAGLALVSGGANLVGVHQVTISTPGSYTAAFGYDWSGQ
                     ISGTAFYDDNSSGTLDGAESGSESVVVMLYSDDNGNGRFDPGIDGQTLVLVTETQADG
                     SYLFDDLPLGDYIVVADEQSVLAPPGSANAGQPFRMVSTTGTKQPVQLTNTQPVATDV
                     DFGFVEGAEIEGSVWYDENHNGILDPGETGIAGVTVWIDLDDDGVLDAGEPTAVTDAT
                     GEYAFIGVVEGTYTIRYDTSDPDIPLQLSDLTTPSSWTQFVQAGAEYEHIDFGRDDGG
                     AIGDTIYADLDGDGVQDPGEPGLAGVAVVLYLDTNGDGVIDADGADNIAGTADDEPAL
                     DTQTTDGAGNYEFAGLADTSGSAEYLVEVQSDSLPGSYETVPTGDPDGVLDGTYSTTL
                     TSGESIDSVDFGYPPEAATVLYSVSGSVFDDNGGGTSANALNGVRDAGEAGLAGIWVY
                     AEVDEDNDGTTDYVATTLTDVNGSYTFFGIAAGSTVVIRVDEETLPGTGYAATNDADG
                     GADGTIQILSLGSNVSGQDFGYFDDPVVAVDDAYVVNEDETLQVVAPGVVGNDVVADG
                     LGGVLLVSGVTNGTLALNPTGGFTYTPNADFSGTDSFTYQVCDTDLDCDIAVVTITVT
                     DVNDLPVANDD"
BASE COUNT          546 a         1128 c         1158 g          639 t
ORIGIN      
        1 gtcgtcgttg gccaccggca ggtcgttcac gtcggtgacc gtgatggtca cgaccgcgat
       61 atcgcaatcg aggtccgtgt cacagacctg gtaggtgaag ctgtcggtgc cgctgaaatc
      121 ggcgttcggg gtgtaggtga acccaccggt gggattgagg gccagcgttc cgttggtgac
      181 tccgctgacc agcaacaccc cgccgagccc gtcggcaacg acgtcgttcc cgacgacgcc
      241 cggcgccacg acctggagcg tctcgtcctc gttaaccacg taagcgtcgt cgaccgccac
      301 gaccgggtca tcgaagtagc cgaagtcctg ccccgagacg ttgctgccca ggctcaggat
      361 ctggatggtc ccgtccgcgc cgccgtccgc atcgtttgtc gccgcatacc cggtgccggg
      421 cagggtctct tcatcgacac ggatgacaac cgtgcttccg gccgcaatgc caaagaaggt
      481 gtagcttccg ttcacatcgg tgagcgtcgt cgcgacgtag tcggtcgtgc cgtcgttgtc
      541 ctcgtcgacc tccgcataga cccagatacc agccagcccg gcttcgcccg cgtcgcgtac
      601 gccattcaat gcgttcgcgg aagtgccgcc gccgttgtcg tcaaagacgc ttccggacac
      661 gctgtagagg accgttgccg cctcgggcgg gtacccgaag tcgaccgaat cgatcgactc
      721 gccactggtc agcgtcgtgg aataggtgcc gtccaatacg ccgtccgggt cgcccgttgg
      781 taccgtttcg taactgccgg gcaggctgtc gctctgaacc tccacgaggt actcggcgct
      841 gccactcgtg tcggcgagcc ctgcaaattc gtagttgccg gctccgtcgg tcgtctgcgt
      901 gtcgagcgcc ggttcgtcgt ctgcggtgcc ggcgatgtta tcggcgccgt cggcgtcgat
      961 caccccgtcg ccgttggtat ccaggtagag cacgaccgcg acgcccgcga gtcccggctc
     1021 gccggggtcc tgcaccccat ccccgtccag gtccgcgtag atggtgtcgc cgatggcgcc
     1081 accgtcatcc cgcccgaagt cgatgtgctc gtactctgcg ccggcttgaa cgaactgggt
     1141 ccagctactg ggcgttgtca agtcgctgag ctggagggga atgtcggggt cactggtgtc
     1201 atagcggatg gtgtaggtcc cctccacgac gccgatgaac gcgtactcgc cggtggcgtc
     1261 ggtgactgcc gtcggctcac cggcgtccag gacgccgtcg tcgtcgaggt cgatccagac
     1321 cgtgacgccg gctatccccg tctcgcccgg gtcgaggatc ccgttgtggt tctcgtcgta
     1381 ccagaccgag ccctcgatct ccgccccttc cacgaatcca aagtcgacat cggtggcgac
     1441 cggctgggtg ttggtgagct ggaccggctg cttggtgccc gtggtggaca ccatcctgaa
     1501 gggctggccc gcgttcgcgc tgcccggggg cgccaggacg ctctgctcat cggcgacgac
     1561 gatgtaatcg cccagcggca ggtcgtcgaa caggtaggag ccgtccgcct gggtctcggt
     1621 cacgaggacc agggtctggc cgtcgatgcc ggggtcgaaa cggccattgc cgttgtcgtc
     1681 cgagtacagc atcaccacga cggattccga ccccgactcg gcgccgtcga gcgtgccgct
     1741 gctgttgtcg tcgtagaagg cggtgccgga aatctggccg gaccagtcgt acccgaacgc
     1801 ggcggtgtag ctgccgggcg tgctgatggt cacctggtga acgccgacga ggttggcgcc
     1861 acccgagacc agcgcgagtc cagcggagaa gtccggatcg gactcgtcga cgtccacgag
     1921 ataagagccc gtgcccagcc ccgagaacga gtacgaccca tcggccgagg tcgtcgtggt
     1981 cgcgacgagc acgtcgcccg aatcgatcaa gccgttgttg ttgagatccg cgtacaggtt
     2041 gacggacacg ccggacagca gcgcctcgcc cggatcggcc gtcccgtcct cgtccacgtc
     2101 gtgccacacc gtgccgccga tcgacgagga gtcctccacc ggcggcggcg gttcgagccc
     2161 gaagtccgcg tcgtcgtact gctggccagc ggcaagaacg attccggcca acgttacatc
     2221 ggtcgtcggg atccaccctg ccggtacggt cgagtagaca tagccgacgc tgtaggtccc
     2281 ggtggtcagg ccgtcgaagg aatagtatcc ggaggcgtcg gtggtggtcg tggcgagcag
     2341 cgcaccggac ccatcgtaga gttccaccgt gacgccggaa atgcccggct ctcccggatc
     2401 ctgcaggccg tccccgtccg tatccttcca gacccggtct ccgatgccgg cagcgagtga
     2461 cgtctgcgtc gtcggactgg acacggacac cagcgcgctg ctggcttccc cgatgagcgc
     2521 cgtgttctgg atcagccccg acgctggcgg cgggttgttg acggtcacct ggaacgtcag
     2581 cacggggctg acgaggctcg tcctcgaagt cgcgctcggg aacgacgtcg atccgtcacc
     2641 cgaggcattg caggtcaggg acaggcttcc cggcaggctg ccggcggtgg cctggtacgt
     2701 ccaccgatac acgaccaggt cgtcggggcc ggagatgtcg tcatcggtgc tcgaaagagt
     2761 gggtcctgtg gagagcgagg cggtggcgcc attcgtgccg gtgatcgagc acgagccggt
     2821 caccacgttg ctgaccggct gcgacgcgct gatcgtcatc tcgaagacga tctcgtcacc
     2881 ggtggtcagc accgagggcg tggccttgat cgaggtctcg tagccttccg gattgagggg
     2941 cggcacgtag gcgagcgcgc cgccctgccc cacgttggct gcgaaattca ccgaggacaa
     3001 ggttgtccat gagttggtag cgatgttgta gcgccagaag accttggtct tgccctggaa
     3061 ggcatagagg tagttgccgc cgtccgtcac caacgctccg ccatcggcca cattgccagg
     3121 ggccgccgcg agggcagacc aggtgttgct cgcgatgtcg tagcggtaga gccctttctt
     3181 cgcgtcgccc tgcagggcgt agatgagtcc accgacgcgt gcaagcgagc cgccccaccc
     3241 gacgttctgg ggcaggctcg ccatggcggt ccaggtgttc gctgcgatgt cgtagcggta
     3301 gaaatctggc ttccggtcgc cgcgtgtcag gtagagatac gtgccgtcgg tcgtgagggc
     3361 gccgcccttc ttgatgttcg ccggggtgct cgcaagcgtc gtccacgagt ccgaggcgac
     3421 attgtacctg tagaaaggca ggccatttcc gcgaacggca aagacgtgtt c
//
LOCUS       DBNHOX010000538         6888 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_623167, whole genome shotgun sequence.
ACCESSION   DBNHOX010000538 DBNHOX010000000
VERSION     DBNHOX010000538.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_623167"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="AC16UZ_11785"
     CDS             complement(<1..949)
                     /locus_tag="AC16UZ_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR6247408.1"
                     /translation="MHSTGIVESFFLIFTGAAVLATAALYSRQPLLVAYIAVGALLGP
                     HGFKLISDSNLLSEISEIGIIFLLFLVGLDLQPSKLKNMVGESLLTAVGTTIVFFAAG
                     FAMMLAFDFSTSEAVVTGIAVTFSSTIIGIKLLPTTVLHHRHTGEIVVSLLLIQDLIA
                     ILALIVLTGYGDTLGATLSSLGMALIALPLLILGALGGMRLLVLPLIRKFDAFNEFIF
                     LLALGWCLGLASIAELAGLSFAIGAFVAGVSLATSPIAQYIAENLRPLRDFFLVLFFF
                     SVGAGLDVMLLMDVFFPTLLLALVLVTVKPGTFALLLNWQ"
     gene            complement(913..1419)
                     /locus_tag="AC16UZ_11790"
     CDS             complement(913..1419)
                     /locus_tag="AC16UZ_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HIR6247409.1"
                     /translation="MNAQLDYIDQPAAQMVSGPGAVPGPARLVAAEQWTPGSSDGLLL
                     AVDAEPEQRFAGAELIAIDFPAFTDGRGLSLAVLLRTRLGFRGELRAVGDIRADMLHY
                     LYRCGFDSFSMSGEGSPVDADGVTKTTAFDALAPYSDCYQASVRQPRPAYRRVERCIA
                     QELSSPSF"
     gene            complement(1403..3064)
                     /locus_tag="AC16UZ_11795"
     CDS             complement(1403..3064)
                     /locus_tag="AC16UZ_11795"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HIR6247410.1"
                     /translation="MYRYDDFDRQFIAERVAHYREQTTRYLAGELSESEFLQLRLRNG
                     LYIQRLAPMLRVAVPYGTLSSGQLRKLAHIARHYDRGYGHLSTRQNMQFNWPELAEVP
                     DILAELAEVDMHAIQTSGSCIRNVTTDQFAGVAADEEVDPRPYCELIRQWSTNHPEFD
                     WLPRKFKIAVTGAASDRAAIAVHDIGLRARREDAEGIVFDVFVGGGLGRTPVIAAEIR
                     KSLPVRHLLTYLEAIMRVYNRYGRRDNKYKARIKILVRAMTAEGFRDRVDAEWAHLKG
                     SESTLTDEEIRRIEAFFTPHEYPPLNDADLGPARLEDPAFAAWLRHNVSPHRVPGYAI
                     VTLSTKVTGVPPGDVSDVQMEAVADWADEYGFGELRLGHEQNFVLPDVPQSRLRELWR
                     RTAALQLGVANVGLISDIVCCPGGDYCSLANAKSIPVAEAIQQRFDDYDYQHDLGELD
                     LNISGCMNACGHHHVGHIGILGVDKKGAEYYQISLGGHSGAGGDEASLGEIIGPSFSR
                     AEVPATIGRILDVYLKRRIDDEPFLHCYRRIGIQPFKDHVYERAA"
     gene            3251..3385
                     /locus_tag="AC16UZ_11800"
     CDS             3251..3385
                     /locus_tag="AC16UZ_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247411.1"
                     /translation="MDDTPTDDARSEADRKADAWAVMIIFWALVAGAVHYVSGWTFDF
                     "
     gene            complement(3382..>6888)
                     /gene="metH"
                     /locus_tag="AC16UZ_11805"
     CDS             complement(3382..>6888)
                     /gene="metH"
                     /locus_tag="AC16UZ_11805"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR6247412.1"
                     /translation="DALNLARPELVADVHRAYLDAGADIIKTNSFNSTRIAQGDYALA
                     DRAEQLNAAAARIARRCADEFASPEWPRFVAGVLGPTNRTASLSPDVNDPGFRNVTFE
                     ELEENYYEAAQALLDNGADLLLLETVFDTLNAKAAIFAIRQLGEKRGATVPLIVSGTI
                     TDASGRTLSGQTCEAFWASVNHAEPLAVGLNCALGADQLRAYVARLGRVADTYLSVHP
                     NAGLPNAFGEYDESPESMAAKLGEFARSGLVNLVGGCCGTTPAHIAAIRESVRECQPR
                     AIPERRNDLMLSGLELLTIDDASLFVNVGERTNVTGSARFARLIREEAYDEALVVARQ
                     QVENGAQVIDINMDEGMLDSQAAMVRFLQLLAAEPDIARVPIMIDSSKWEIIEAGLRC
                     VQGKAIVNSISLKEGEDAFEAVARRCRDYGAAVVVMAFDEDGQADTLERKREICKRSY
                     RILTERIGFPPQDVIFDPNVFAIGTGIDEHRGYAVSFIEACRWITDNLPHARVSGGIS
                     NVSFSFRGNNPIREAIHTVFLYHAIRAGLTMGIVNAGQLGIYQEIPDELREKVEDLVL
                     NRRSDATERLLAVADRYGGRASESEGPDLSWREGSVAQRLGHALVKGIGEFIVEDTEE
                     ARLAADHPIEVIEGPLMDGMNVVGDLFGQGKMFLPQVVKSARVMKQAVAHLVPYIEAA
                     QGPGRKAKGRIVMATVKGDVHDIGKNIVGVVLQCNNYEVIDLGVMVPMEKILDTAQEV
                     GADVIGLSGLITPSLDEMVQVAGEMQRRGLNLPLMIGGATTSKAHTAVRIEPAYQDGP
                     TVYVSDASRSVSVAARLVSPQQRIGFSEETRKEYEEIRRRTSARSANRKYLSLEAARR
                     NAFVCNWEAYEPPRPASIGVQTIENLPLETLVPYIDWSPFFLTWELAGKYPAILDDPV
                     VGESARDLFRDASQMLEHLCREKRLKANAAFGFWPCNRTGPEELTLFEDEERKTELAR
                     LQHLRQQTIRGAGRPNYSLADFVGPPPVADYIGAFAVTAGIGIEDVLADYGDDDYGAI
                     MIKALADRLAEAFAEYLHEQVRQVHWGYAPGESLAAEELIGEAYRGIRPAPGYPACPE
                     HSEKSTLFRLLDAQANAGMTLTESFAMLPAASVAGWYFSHPDARYFGIGKIDDEQVAD
                     YASRKGITALEAEKLLRPNLDR"
BASE COUNT         1217 a         2122 c         2296 g         1253 t
ORIGIN      
        1 cctgccagtt gagcagcagg gcgaacgtgc ccggcttgac ggtaacgagg accagcgcca
       61 gcagcagcgt ggggaagaaa acgtccatca ggagcatgac gtcgagaccg gctccaaccg
      121 agaagaagaa gagaaccaga aagaagtctc gcagtgggcg caggttctcg gcgatgtact
      181 gcgcgatggg gctggtggcc agggaaacac cggccacgaa agcgccgatg gcaaacgaca
      241 ggccggcaag ctccgcgatg ctggcaaggc ccaggcacca gcccagcgcc agcagaaaaa
      301 tgaactcgtt gaaggcgtcg aattttctga tcagcggcaa taccagcagg cgcatgccgc
      361 ccagcgcgcc gaggatgagc agcggtagcg cgatgagcgc catcccgagg ctcgacagcg
      421 tggcgcccaa cgtatcgccg tagcccgtta agacgatgag agcgaggatg gcgatcaggt
      481 cttgaatcag caggaggctg acgacgatct cgcccgtatg acggtgatgc agaaccgtgg
      541 tggggagcag cttgatgccg atgatggtgc tggaaaacgt caccgcgatg ccggtgacca
      601 cggcctcgct cgtggaaaag tcgaaggcga gcatcatcgc gaaacccgcg gcgaaaaaca
      661 cgatcgtggt gcccacggcg gtcagcaggg attcgcccac catgttcttc agcttgctgg
      721 gttgaaggtc caggcccacg agaaagagca gaaagatgat gccgatctcg gagatttccg
      781 aaagcaggtt agagtctgaa atcagcttga acccgtgagg tcccagcaga gcaccgacgg
      841 cgatgtaggc gacgagcagc ggctggcgcg agtacagggc cgcggtggct agcaccgcgg
      901 cgcccgtgaa tatcagaaag aaggactcga caattcctgt gctatgcacc tctcgaccct
      961 tcggtaagct ggacggggct ggcgtaccga agcttgatag caatccgagt agggtgcaag
     1021 ggcgtcgaag gccgtggtct tggtcacgcc gtcggcgtct acggggctgc cctcgccgga
     1081 catggagaag ctgtcgaagc cgcaacgata aaggtagtgc agcatgtcag cccgaatgtc
     1141 gcccacggcg cgcagctcgc ctcggaaacc gagacgcgtt cggagcagca cggccagcga
     1201 gagtccgcgg ccgtccgtga atgccggaaa gtcgatggcg atcagttccg ccccggcaaa
     1261 ccgctgctcc ggttcagcgt cgacggcgag gagcagaccg tcggacgatc cgggcgtcca
     1321 ttgctcggcg gcaaccagcc tcgccggacc cggcacggct cccggtccag acaccatctg
     1381 cgcggcgggt tggtcgatgt agtcaagctg cgcgttcata gacgtgatcc ttgaaaggtt
     1441 ggatgccgat gcggcgatag cagtgcagga aaggctcgtc gtcgatgcgc cttttcaggt
     1501 agacgtcgag gatcctgccg atggtcgcgg gaacctcggc gcgggaaaag gatgggccga
     1561 tgatctcgcc gagagacgct tcgtctccgc cggcacccga atgcccgccc agcgagatct
     1621 ggtagtactc cgcgcctttc ttgtcgacgc ccaggatgcc gatgtgaccc acgtggtggt
     1681 gcccgcaggc gttcatgcag ccggagatgt tcaggtcgag ctcacccagg tcgtgctggt
     1741 agtcgtaatc gtcgaaacgc tgctgaatgg cttccgcaac gggaatggac ttggcgttgg
     1801 ccagagagca gtagtcgccg ccggggcagc agacgatgtc ggaaatcagc ccgacgttgg
     1861 cgacgcccag ctggagcgcc gcggttcttc gccacagttc ccgaagccgg ctctggggga
     1921 cgtcgggcag cacgaagttc tgctcgtggc ccaatcgcag ctccccgaac ccgtactcgt
     1981 ctgcccagtc ggccacggcc tccatctgca cgtcggaaac gtctcccggg ggcacgcccg
     2041 tcaccttcgt ggacagcgta acgatggcgt agccgggtac ccggtgggga gagacgttgt
     2101 ggcgcagcca ggcagcaaag gcgggatcct ccaggcgcgc gggtcccaga tcggcatcgt
     2161 tcagcggcgg atactcgtgg ggggtgaaaa aagcctcgat gcggcggatt tcctcatcgg
     2221 tcagcgtgct ttccgagccc ttcagatggg cccactcggc atccacccgg tcgcggaaac
     2281 cctccgcggt catggcccgt accagaatct tgattctggc cttgtacttg ttgtctctgc
     2341 ggccgtagcg gttgtagacg cgcatgatgg cctcgaggta ggtcagcaga tgcctcaccg
     2401 gaagcgactt gcggatctcg gcggcgatga cgggcgtgcg cccgaggccg ccccccacga
     2461 acacgtcgaa cacgatgccc tcggcgtcct cgcggcgcgc gcgcaagccg atgtcgtgga
     2521 cggcgatggc tgctcggtcg gaagcagccc cggtgacggc gatcttgaac ttgcgcggca
     2581 gccagtcgaa ctccgggtga ttggtcgacc actggcggat gagctcgcag tacggtctgg
     2641 gatcgacttc ctcgtcagcc gcgacgccgg cgaactggtc ggtcgtcacg ttgcgtatgc
     2701 agctgccgct ggtctggatg gcgtgcatgt cgacctcggc cagctctgcg aggatgtcgg
     2761 gtacctcggc gagctcgggc cagttgaact gcatgttctg acgcgtgctg aggtgaccgt
     2821 aaccgcggtc gtagtggcgc gcgatatggg ccagcttgcg cagctggccg gagctgagcg
     2881 tgccgtaggg gacggccacc cgcagcattg gagcgagtcg ctgaatgtac aggccgtttc
     2941 ggagcctgag ctgcagaaac tcgctctcgg acagctcgcc ggcaagatag cgggttgtct
     3001 gctcgcggta atgggcaacg cgttccgcaa tgaactgcct gtcgaagtcg tcgtatcgat
     3061 acatctggct ggctggtaat cggcgctcag cgcgaagcgc aggccgcgtc tcgtcatctt
     3121 tgaggcaggc gaatctaaca ttcggccctt gccgctcgaa gtaaccgttc ggactatgct
     3181 tagaaccgtc tttttcggta gactcgcgcg ccgtgcgctg ggcacgatca actcgctggt
     3241 gaggagcacg atggacgaca cccccacaga cgatgcaaga tctgaagcgg atcgcaaggc
     3301 tgacgcctgg gccgtgatga tcattttctg ggcccttgtc gccggggccg tgcactacgt
     3361 ctccggctgg accttcgatt tctagcgatc gaggttgggc ctgagcaact tctccgcttc
     3421 cagggcggta attcctttcc tgctggcgta gtcggcaacc tgctcgtcgt cgatcttgcc
     3481 gatgccgaaa tatcgtgcgt cggggtggga gaaataccag cccgctactg aggccgccgg
     3541 cagcatggcg aagctttcgg tcagcgtcat gccggcgtta gcctgggcgt cgagcaggcg
     3601 gaacagcgta cttttttcgc tgtgctcagg gcacgcggga tacccgggcg cgggccggat
     3661 gccccggtag gcttcaccga tcaactcctc ggctgccagg gattcaccgg gcgcgtaacc
     3721 ccagtgcact tgacggacct gctcgtgcag gtactcggca aacgcctctg ccaacctgtc
     3781 ggcaagggcc ttgatcatga tggcgccgta gtcgtcgtcg ccgtagtcgg caagaacgtc
     3841 ctcgatgccg atcccggcgg tcacggcgaa agcgccgatg tagtcggcca ccgggggcgg
     3901 tcccacgaag tcggccaggc tgtagttcgg gcgccccgcg cccctgatcg tctgctgtcg
     3961 gaggtgctgc agcctggcga gctccgtttt gcgctcctca tcctcgaaca aggtcagctc
     4021 ctcgggcccc gtgcggttgc atggccagaa tccaaacgcg gcgttcgcct tcaggcgctt
     4081 ctcgcggcag aggtgttcga gcatctgcga ggcgtcgcga aacagatccc gggcgctctc
     4141 gcccaccacg ggatcgtcca ggatcgcggg gtatttgccg gccagttccc aggttaggaa
     4201 gaacggcgac cagtcgatgt agggaaccag ggtctccagg ggcaggttct cgatggtctg
     4261 gacgccgatc gaggccggtc tgggcggttc gtaggcctcc cagttgcaga cgaaagcgtt
     4321 gcgtcgggcg gcctccaggc tgagatactt ccggttggcc gagcgggcag acgtgcgccg
     4381 gcggatctcc tcgtattcct ttctggtctc ctcgctgaag ccgatgcgct gctgtggaga
     4441 gaccaggcgt gcggcaacgc tcacgctgcg cgaggcgtcg gaaacgtaga cggtggggcc
     4501 atcctggtaa gccggctcga tcctgaccgc cgtgtgggct ttggaggtcg ttgcgccacc
     4561 gatcatcagc ggcaggttca ggccgcgtct ttgcatttcg ccggccacct gcaccatctc
     4621 gtctaaggag ggggtgatca gcccggaaag cccgatgacg tctgcgccca cctcctgggc
     4681 agtgtcgaga attttctcca tgggcaccat gacgcccagg tcgatcacct cgtagttgtt
     4741 gcactgaagc acgacgccga cgatgttctt gccaatgtcg tggacgtctc ctttcacggt
     4801 cgccatgacg atgcggcctt tggctttccg acccgggccc tgtgcggctt cgatgtaggg
     4861 caccaggtgg gccaccgcct gtttcatcac gcgcgcgctc ttgaccacct ggggtagaaa
     4921 catcttcccc tggccgaaca ggtcgcccac cacgttcatg ccgtccatca gggggccctc
     4981 gatcacctcg atgggatgat cggcggcgag ccgggcttcc tcggtgtcct cgacgatgaa
     5041 ctcgccgatg cctttcacca gggcatggcc gagtcgctgg gccacgctgc cttcccgcca
     5101 ggaaaggtcg ggcccttcgc tctccgaggc cctgccgccg taccggtcgg caaccgccag
     5161 cagacgctcg gtggcatcgg atcggcgatt gagcaccagg tcttccacct tctcgcgcag
     5221 ctcatcggga atctcctggt aaattcccag ctgaccggca ttgacgatgc ccatggtcag
     5281 cccggcgcga atggcgtgat acaggaagac cgtgtggatc gcctcgcgaa tggggttgtt
     5341 gccgcggaag gagaaggaga cgttgctgat gccgcccgag actctcgcgt ggggcaggtt
     5401 gtcggtgatc cagcggcacg cctcgatgaa gctgaccgcg tagccgcggt gctcgtcgat
     5461 gccggttccg atggcaaaga cgttgggatc gaagatcacg tcctggggag ggaacccgat
     5521 ccgctccgtc aggatgcggt aggaacgctt gcagatctcg cgcttgcgct ccagggtgtc
     5581 ggcctggccg tcctcgtcga aggccatcac caccacggcc gcgccgtagt cgcgacatcg
     5641 tcgagccacg gcctcgaagg cgtcttctcc ctctttgagg ctgatcgagt tgacgattgc
     5701 cttgccctgc acgcagcgaa gaccggcctc gatgatttcc catttgctgg agtcgatcat
     5761 gatcggcacg cgagcgatgt cgggctcggc ggcgaggagc tggagaaagc gcaccatggc
     5821 ggcctggctg tccagcatcc cctcgtccat gttgatatcg atgacctggg ccccgttctc
     5881 cacctgctgg cgggctacga cgagcgcttc gtcgtaggcc tcttcgcgta tcagccgggc
     5941 aaaacgcgcg gagcccgtca cgttggtccg ctcgcccacg ttgacgaaga gtgaggcgtc
     6001 gtcgatggtg agcagctcca ggccgctgag catcagatcg ttgcggcgct cgggtatcgc
     6061 tctgggctgg cattcccgca ccgactctcg aatggcggca atgtgcgccg gcgtcgtgcc
     6121 gcagcacccg cccaccagat ttaccagccc gctgcgggca aactcgccga gcttggcggc
     6181 catggactcg ggcgactcat cgtactcgcc gaacgcgttg ggcaggcccg cgttggggtg
     6241 gacgctgagg taggtatcgg caacgcgccc caggcgcgcc acgtaggcgc gcagctgatc
     6301 ggctcccagc gcgcagttca gccccacggc caggggctcg gcgtgattga cggaggccca
     6361 gaacgcttcg cacgtctggc cggaaagcgt tcgcccgctg gcgtcggtaa tcgttccgga
     6421 gacgatgagc ggcaccgtcg cgccccgttt ctcgcccagc tggcggatcg cgaaaatcgc
     6481 cgctttcgcg ttcaaggtgt cgaacacggt ttcgagcagt agcagatccg caccgttgtc
     6541 gagaagcgct tgcgcggctt cgtaatagtt ctcctctagc tcctcgaagg tgacgttgcg
     6601 gaacccggga tcgttcacgt ccggagagag gctcgccgtg cggttggtgg gcccgagcac
     6661 gcccgcgacg aagcgcggcc attcgggcga ggcaaactca tcggcacagc gtcgggcgat
     6721 ccgtgcggcg gcggcgttaa gctgctccgc gcgatcggcc agcgcgtagt cgccctgagc
     6781 gattctcgtg gagttgaagc tgttggtttt gatgatgtcc gcccccgcgt ccagataggc
     6841 gcgatggacg tctgcgacca gctcgggacg ggccagattg agggcatc
//
LOCUS       DBNHOX010000539         2814 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_494967, whole genome shotgun sequence.
ACCESSION   DBNHOX010000539 DBNHOX010000000
VERSION     DBNHOX010000539.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_494967"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="AC16UZ_11810"
     CDS             <1..238
                     /locus_tag="AC16UZ_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HIR6247413.1"
                     /translation="VRSGIVSRPPDLDWGMLAGGGLLAGIGFTMALFIADLAFDDALI
                     NAAKLGILSASVASAAAGLAVLAWSTRAKSVSLG"
     gene            complement(528..2705)
                     /locus_tag="AC16UZ_11815"
     CDS             complement(528..2705)
                     /locus_tag="AC16UZ_11815"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF023853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III family protein"
                     /protein_id="HIR6247414.1"
                     /translation="MSMKARRDAIRDIVAPDRPEALSAASVEDVFGCQVFTIDRMRRV
                     LPDGVCKKLFATIERQEALDASIADAVAIAMRDWAVEHGASHYTHWFQPLTGLTAEKH
                     ESFVTPTVGGGAIAKFSGSALIQGEPDASSFPSGGLRTTFEARGYTAWDPTSPAFIYR
                     NRNGSVLCIPTAFVAWTGEALDKKTPLLRSNQALGREALRALRLFGSDSGVYTVVATC
                     GAEQEYFLIDRNFYFARPDLMTCGRTLFGARPPKGQELEDHYFGQIPERVLSFMLDSE
                     NELYKLGVPVKTRHNEVAPAQYEIAPIFEDANVANDHQQLTMTVLASLAPRYGLQALL
                     HEKPFAGVNGSGKHLNWSMATNTGTNLLDPGATPHQNMQFLFFCTAVIRAAHRHADLL
                     RIAVASAANDHRLGANEAPPAIMSIFLGEQLQEIFQKIQEGGSATGRAAGFMGLGLDQ
                     LPPLPMDSGDRNRTSPFAFTGNRFEFRAVGSSQSIAGPSVVLNTIVADSIAFMTTKLE
                     KDLEGGAGLEEALHGLIREVMQEASVVLFEGDGYSQEWHEEADRRGLPNHPDTVEALH
                     HLLDTKNVEVFEKTGVLSQRELESRLEIYADQYIKTLNIEAETTASLARTMILPAAIE
                     FQERVSRSVNAAAAAGCDPEGARAVLGEVCDGVTKLKAALDRLERVNAPPRPASRLEH
                     ARLMRDEVLPDQNAVRAAADRLEKLMPDDLWPLPTYRDLLFIK"
BASE COUNT          474 a          901 c          942 g          497 t
ORIGIN      
        1 ggtgcgttcg gggatcgtga gtcgcccccc ggacctggat tggggaatgc tcgccggagg
       61 gggtctgctc gccgggatcg gcttcacgat ggcgctgttc atcgccgacc tggcgttcga
      121 cgatgcgctg atcaacgcgg ccaagctggg catcttgtcc gcatcggtcg cttctgcggc
      181 ggccggtctg gccgtgctgg catggtcgac gagggccaag tccgtgagcc tcggctgatc
      241 gcccatccga ccactcgaga gcgggcacga aggcccgccg tggcccgcgc cacgaatcga
      301 ttccgcaagg agagttcgtc gagcgatgga atcatcagga agatgacgtg cgcttgcccc
      361 ctcaccaagg agccactgcg aaggcctcag ccggatcgat ggccgtggcg aggagcgatc
      421 gtaatagaac gcccttggag aaggggaggc catccccggg gccccggatc gagatttcgg
      481 ccgagccttc tgaagccaac ccgaaaccct cccgtgggct ccgtcgatca cttgatgaac
      541 agcaggtcgc ggtaggtcgg caggggccag agatcatccg gcatcagctt ctcgagccga
      601 tccgcggccg cccgcaccgc gttctggtct gggagtacct cgtcgcgcat gagcctggcg
      661 tgctcgagcc gcgaggccgg gcgcgggggc gcgttgacgc gctccaggcg gtcgagggcg
      721 gccttgagct tcgtgacgcc gtcgcagacc tcccccagca ctgctcgcgc tccctccgga
      781 tcgcagccgg cagcggcggc cgcgttgacg gagcgcgaca cccgttcctg gaactcgatg
      841 gcagccggca ggatcatcgt ccgggcaagc gaggcggtcg tctcggcctc gatgttgagg
      901 gtctttatgt actggtccgc atagatctcg agccgagact cgagctcgcg ctgcgaaagc
      961 acgccggtct tctcgaacac ctccacgttc ttggtgtcga gaaggtggtg cagcgcctcg
     1021 accgtatccg gatggttcgg gaggccgcga cgatcggcct cttcgtgcca ttcctgcgaa
     1081 tagccgtcgc cctcgaatag aacgaccgac gcttcttgca tgacctcgcg aatcagcccg
     1141 tgcagggcct cctccagccc ggctccgccc tccaggtcct tctcgagctt cgtggtcatg
     1201 aaggcgatcg agtccgcgac gatcgtattc agcacgacgc tgggccccgc gatcgactgg
     1261 ctcgatccca ccgcgcgaaa ctcgaagcgg ttgccggtga acgcgaaggg gctcgtccgg
     1321 ttgcggtctc ccgagtccat cgggaggggc gggagctgat ccaagccgag ccccatgaaa
     1381 ccggccgccc gaccggtggc ggatccgccc tcctggatct tctggaagat ctcctgaagc
     1441 tgctcgccca ggaagatcga catgatggcg ggcggagcct cgttcgcacc gagccggtga
     1501 tcgttggcgg cactggccac cgcgatgcgc agcaggtcgg cgtgtcggtg agcggcgcga
     1561 atcaccgccg tgcaaaagaa gagaaactgc atgttctggt gtggcgtcgc gcccggatcg
     1621 agcaggttcg tgccagtatt ggtggccatc gaccagttga gatgctttcc cgagccgttc
     1681 acgcccgcga agggcttctc gtggagaagc gcctggaggc cgtaacgagg cgccaggctc
     1741 gcaagcaccg tcatcgtcag ttgctggtgg tcgttcgcca cgttggcgtc ttcgaagatc
     1801 ggcgcaattt cgtactgggc cggtgcgacc tcgttgtggc gtgtcttcac gggaacgccg
     1861 agcttgtaga gctcgttctc gctgtcgagc atgaacgaga ggacgcgttc cggaatctgc
     1921 ccgaagtagt ggtcctcgag ctcctggccc ttcggcggtc gggccccaaa gagggttcga
     1981 ccacaagtca tcagatccgg gcgcgcaaag tagaagttcc gatcgatcag gaagtactcc
     2041 tgctccgcgc cgcaggttgc gaccaccgtg tacacgccgg agtcggaccc gaacaggcgg
     2101 agcgcgcgca gcgcttcgcg cccgagcgcc tggttggaac gcagcaaggg cgtcttcttg
     2161 tccaacgcct caccggtcca cgcgacgaag gcggtgggga tgcagagcac gctgccgttt
     2221 cgattccggt agatgaaggc ggggctggtc ggatcccacg ccgtatagcc acgcgcttcg
     2281 aaggtggtgc gcagccctcc agacggaaac gagctggcgt ccggctcccc ctggatcagc
     2341 gcgcttcccg agaacttcgc gatcgctccc ccacccaccg tcggcgtgac gaaggactcg
     2401 tgcttctctg ccgtcagacc ggtgagcggt tggaaccagt gggtgtagtg gcttgcgccg
     2461 tgctctacag cccagtcccg catggcgatg gccacggcgt cggcgatcga ggcgtcgagg
     2521 gcctcctgcc gttcgatcgt tgcaaacagc ttcttgcaca cgccatcggg aagaacgcgg
     2581 cgcatgcggt ctatcgtgaa cacctgacat ccgaacacgt cttcgacgga agctgccgac
     2641 aaggcttccg gccggtccgg ggcgacgatg tctcgaatgg catctcgccg ggctttcatt
     2701 gacatgcttc tctccgacgc tcgcccgcgt cgaacgaccg tgggaggcgg ataccgcaga
     2761 aggcgagccg ggcaaagagg atcagagcgg gcgtagaata gcgccccgtg agtt
//
LOCUS       DBNHOX010000540        14792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_815211, whole genome shotgun sequence.
ACCESSION   DBNHOX010000540 DBNHOX010000000
VERSION     DBNHOX010000540.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 14792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14792
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_815211"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="AC16UZ_11820"
     CDS             complement(<1..600)
                     /locus_tag="AC16UZ_11820"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6247415.1"
                     /translation="MLKLYLMPGSCALASHITLREAGADFELKVVDFMSGEQRTPEYR
                     KVNPKGRVPALVTDRGVLTETPAILLYVAQTHPEARLAPLDDPFELARLQAFTSYLCA
                     TVHVAHAHSRRGERWADDESAIEAMKRKAPGNMADCFQLIEDQFFEGPWLMGEQFTVA
                     DPYLFTITRWLKFHQIDSSGYPRVHDHLGRMGERPAVRAA"
     gene            725..1639
                     /locus_tag="AC16UZ_11825"
     CDS             725..1639
                     /locus_tag="AC16UZ_11825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6247416.1"
                     /translation="MQIGIHAPHLGRELDPVTVRSFVQECDTLGVHSLWVSDHVCWPA
                     DIASKYPYTEDGSFPATPEMGWLDPIGTLLFIAGCTDRVRLGTTVLILPYRPPVATAK
                     QLATLDVVSGGRLILGVGIGWMAEEAAVLGMPWDHRGRRSDEQLEIFRRLFTETEPSF
                     EGEFYSFPKVGFEPKPVQQPVPVWVGGSTPAAFRRVARFGQAFHAAFQPLAEVGREWR
                     AVQAACEEIGRDPSDLSLSLRLYLDPDSRMEPEKSVAGSAEQMRERLFELAAAGVGHV
                     VLDPVARGGVQGRLDAVRGFMTDVAPAS"
     gene            1711..2208
                     /locus_tag="AC16UZ_11830"
     CDS             1711..2208
                     /locus_tag="AC16UZ_11830"
                     /inference="COORDINATES: protein motif:HMM:NF016213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HIR6247417.1"
                     /translation="MDSAMLIDVLLWILVVGLVIVGVAGLLLPALPGAPLVFAGLLIG
                     AWIDDFAYVSWGTLITLGIMAALTYAVDFAAGALGASRFGASPRAVWGAVIGGIVGIF
                     MGLVGIIIGPFVGAVIGELSAQRSLEEAGRAGLGATLGLVIGTAIKLTLAMAMVGIFV
                     LMRFL"
     gene            complement(2238..3035)
                     /locus_tag="AC16UZ_11835"
     CDS             complement(2238..3035)
                     /locus_tag="AC16UZ_11835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6247418.1"
                     /translation="MTEEASRFVGSIPEHYDAGLGPHIFEGYAEDLAGRVAALDPAAV
                     LELAAGTGIVTRRLRDVLPAGCDIVASDLNQPMLDFAEAKFAPDESVGFEVADATDLP
                     WQDERFDVVTCQFGVMFFPDKHRSYAEARRVLRAGGHYVFNVWDAWPFNAFAEITHDV
                     VAGFFPDDPPGFYRVPFGYHDVEVIRRSLLDAGFRAASAETVQLTSAIPSAENFARGL
                     VFGNPLFQEIEQRGGDADAIHAAVADALARRLGSQMQIRAIVVHAQK"
     gene            complement(3028..3204)
                     /locus_tag="AC16UZ_11840"
     CDS             complement(3028..3204)
                     /locus_tag="AC16UZ_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247419.1"
                     /translation="MKSWIDFRENGSSPLTKIKREYFGELRLVWKEELGNYHVRQATA
                     LVGGGGRSGEVSFD"
     gene            3349..5571
                     /locus_tag="AC16UZ_11845"
     CDS             3349..5571
                     /locus_tag="AC16UZ_11845"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020298890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HIR6247420.1"
                     /translation="MRAFIWIALVAIAAVVYFFATSTAPDGATEDGPTAAGGGTLESG
                     PSVGLIDDARINAADSEPGNWLAYGRTYEEQRFSPLTQINRESVGELGLAWYKDMGTN
                     RALEATPIVVDGIMFFTTTWSRVHAVDARTGETVWFFDPEVPGETGRKACCDVVNRGV
                     AVYQGKVFVGSLDGRLIALDAATGEKVWEVDTIVDRTRAYTITGAPRAARGKIFIGNG
                     GAEYGVRGYVTAYDAQTGEQVWRFYTVPGDPSKPFEHPEMEMAAKTWKGGEWWEIGGG
                     GTVWNSIVYDPDFNHVYLGVGNGSPWTRVIRSPGGGDNLFLSSIVALNADTGEMQWYY
                     QTTPGDNWDYTAVQDIALADMEVDGEMRKVLLQAPKNGFFYVIDRTNGELLRANPYVA
                     VNWATHVDMATGRPVENPAMDYADTAKWILPGPLGGHNWQAMSVDVEAGLVYIPALEN
                     PLIYGMPDEWQQTGVYKRDPGRWNTGIELGAIAQLFVDNIAEQPTPRGLLKAFDPLSG
                     EERWSVEIPHYWNGGVLGTAGGLVFQGNAMGMFKAYDKSTGEALWEFNTYTSMLAPPV
                     TFELDGEQYVSILTGFGGGDLFGGEPLPPLSMHASLKYNNHGRLLVFKLGAKEQLPVP
                     SARDRTIPEQLVADVDVADIARGERLYNDYCAVCHGLVARSSGAIADLRLMSAESHDA
                     FDGVVLEGLMSARGMTAFSDVLTVEDSERIHHYVRARAHEDREVALGNSEAARLTWLN
                     "
     gene            complement(5575..6141)
                     /locus_tag="AC16UZ_11850"
     CDS             complement(5575..6141)
                     /locus_tag="AC16UZ_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247421.1"
                     /translation="MTGPILGLTLAYVAVAALLLCLNLATRFSPWVKGIAVVLVTALY
                     ASAWLGYQGLLGWASPAALPETFRVLWITVEEPDKESGAPGTIFFWVRELDEAGFPVA
                     PPRAHRIAWDEASARAAQEALEKLEEGERLNGRLSRGVVDEGEGRPEETTGYAAEQSV
                     TGAVGRRPDFEFVQVPPPALPPKAVPGG"
     gene            complement(6138..6755)
                     /locus_tag="AC16UZ_11855"
     CDS             complement(6138..6755)
                     /locus_tag="AC16UZ_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247422.1"
                     /translation="MRHDVFRQRESQDAFTDLLFNALLGFAFMFVAAFALISDPSNTG
                     KIDSKAEVLITVRWADRHPDDVDTLVEGPRGQLVWYHNRDSGLMHLDRDDRGLFADRI
                     VLDGREVSNPINQETVTVRALQPGEYVVNVLHYQSNYSEPLPVTVKVEKLNPTVTLVY
                     YGTRQLTGVGDEQTAVRFTIGADGQVASTSELPKPLLTRAVGGKT"
     gene            complement(6745..7413)
                     /locus_tag="AC16UZ_11860"
     CDS             complement(6745..7413)
                     /locus_tag="AC16UZ_11860"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6247423.1"
                     /translation="MFKLTDANLVFRALVIFGLVLFGFFLAEQQGLVSLALGSDKSRI
                     SYLILGLYGAVSVHWLWLARALGAERRRFVALENSICEGEAVDVRGGLLGGFLENLSL
                     KRDDDASALLEAFGDELANRHAFGHFASDVLLKLGLLGTVVGFILMLLPVGEIEEFDP
                     SLMQQLLSAMSGGMAVALYTTLAGLVTSTLLKLQYHLLDASAAQLVTRLAVLVDVRLK
                     RDAA"
     gene            7721..9532
                     /locus_tag="AC16UZ_11865"
     CDS             7721..9532
                     /locus_tag="AC16UZ_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012946.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6247424.1"
                     /translation="MSRSEVEGAEFGFDPEALRAKYAEERDKRLRADGNAQYLEVTGK
                     FAHFLDDPYVERTPRDALTDEVEVAIIGGGFGGLLAGARLREAGVEDLRIIEKGGDFG
                     GTWYWNRYPGAQCDIESYIYLPLLEEVGYIPREKYSFAPEIMAHSQAIGRKYDLYRNA
                     CFQTEVTELRWDEEDQRWEIRTNRGDRMKARFVCMSNGPLNRPKLPGIEGIESFQGHT
                     FHTSRWDYAYTGGDPNGNLENLAGKRIGIIGTGATAVQCVPHLAETSEHLHVFQRTPS
                     SIDARGNRPTDPGWAAKLAPGWQRDRMHNFNVLVTGGYQEEDLVSDGWTDIIRNLGII
                     SARRAQTESLTLEDMAKNVELADFQKMEQIRARVDELVDDPETAEALKPYYRQFCKRP
                     CFHDEYLQAFNRPNVTLVDTDGRGVERITDKGIVANGVEYELDCIIFATGFEVGTSYS
                     RRAGYEIVGRNGLTLTDKWADGMKTLHGFQTNGFPNCFFMGVTQGGFTANFPHLLNEQ
                     SIHIAYMIDQARNKNAHVLEASPEAEAGWVETIRQMAVFNQNFLESCTPGYYNNEGNP
                     EVARSLLGSQYGGGPEAFFQILRDWREEGGMEGLDFS"
     gene            9572..10450
                     /locus_tag="AC16UZ_11870"
     CDS             9572..10450
                     /locus_tag="AC16UZ_11870"
                     /inference="COORDINATES: protein motif:HMM:NF004843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6247425.1"
                     /translation="MNDFEGKTAVVTGAASGIGLALAERFAAAQMQVVLADIEEAALD
                     QAVKRLEERQARVIGVVTNTMVESSVAELAERALGEFGNVHVLCNNAGVASLGAGGRG
                     IWEVPAADWDWVMGVNFYGVLFGIQAFVPHMLEHGEPGHIVNTASLAGLFPGGGTYGV
                     SKHAVLALTETLYNDLKARGAALSASVLCPGFVSTRIAKAERNRPAGLEIDQGDVSSE
                     MAAAAEALIAQGKDPAEIAEAVFEAIAQDNLYVLPHPAWDDFVRARVDHVLDRRGPAT
                     MDPAELMRRRAGGEAL"
     gene            complement(10447..11193)
                     /locus_tag="AC16UZ_11875"
     CDS             complement(10447..11193)
                     /locus_tag="AC16UZ_11875"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6247426.1"
                     /translation="MKLLVTGLSGLIGGAAHPVLAADHDVAALNRREVNGTFTWQADI
                     ADYEAIRPAFDGVDAVIHLAAKPGEHFGWDALRVTNVEGTRNVFQAAVEAGVPRVVFA
                     SSGATVAGWEHDEPYRALVEGRYDDLPDRWPMITVDAPPRPRGVYGSTKVWGEALARH
                     FADTTATSFISVRIGFVNAEDRPANARQRSVWCSQRDVVNALVAALGAPEAVRCGTFF
                     ANSRNRYGYRDLDHGRDLLGFEPLDAAEAG"
     gene            complement(11190..12149)
                     /locus_tag="AC16UZ_11880"
     CDS             complement(11190..12149)
                     /locus_tag="AC16UZ_11880"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6247427.1"
                     /translation="MDEIVPRKIRDPRIETTFVDANGLRFEVDACGSGRRLALCLHGF
                     PEHSFSWRHQLPMLADLGFRAWAPNLRGYGLTSRPFGVDAYAIENLMDDVAGLIDASG
                     CRETVLIAHDWGALVAWYFAMRKVRPLEKLIICNVPHPGAGRASGRSFAQLRKSWYVF
                     FFQLPRLPENLLGRRGARAVADAFRGVAVDPSRFPDEVVDVYRRNAAQPGALEAMINY
                     YRAALRGGGFRRQSALGFPVIETPTLMVWGEEDAALGIETTRGTDDYVANLTLRYLPR
                     VSHWVQQEQPEVVNAMMRAFLENEPVPYLTWEPKLVQAVKEEA"
     gene            complement(12170..12814)
                     /locus_tag="AC16UZ_11885"
     CDS             complement(12170..12814)
                     /locus_tag="AC16UZ_11885"
                     /inference="COORDINATES: protein motif:HMM:NF044444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6247428.1"
                     /translation="MNVKSTRVRRNPEAARAEILAAAMAALQDVRFSELTVDSLMKRT
                     GMTRSSFYHYFSGLDEIALGMLEEFAADIRRTVDPWLTGELDEPDHRAATLTHLTSMF
                     EVFERHAPSVAAVAQAAGGSQEVYQEWQRGIVDYFIDLTAEFIRRQRALGRSGAADPD
                     RLARALILMSNAAANDNMTRHNPDGPAVIGAVLGDIWNSAIYGPGGDGAHRVPG"
     gene            12939..14465
                     /locus_tag="AC16UZ_11890"
     CDS             12939..14465
                     /locus_tag="AC16UZ_11890"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6247429.1"
                     /translation="MNVLASQLEPWAVADRQPRIAIIGAGMSGIAAVVKLRKAGYTHL
                     TVYEKADRVGGTWRENTYPGLSCDVPSRWYSFSFALSSEWTHRFSYGPEIQAYMEKVA
                     EGFGVTDVVRFDTPVTELTYEAPFWRLTTAAGEQQIYDVVISATGILHHPAMPDIEGL
                     EDFAGASFHSARWDHSVDLTGKRVGVIGTGSTAAQIVGDIAGSVGELHVFQRTPQWMV
                     PMPQKTYSARWKRVLKLFPFLKRLAYQYYFRLMVRTFAMATVGDEKMQARISKITRQN
                     LERNVKDPELRRRLTPDYQAACKRLILCSDFYPAITRDNAHLVTDAIERVTPAGIRTA
                     DGRVHELDVLVLATGFDPSAFILPTKVTGEDGVDLESFWQGAPRALRAVAIPRFPNFW
                     MLEGPTGPVGNMSLITITEHQVGYVIAMLERMKKRRLVAVSAREDAYRNYNQAMQEAV
                     KNTVWMTGGCTSWYIDKTGIPNLYPWEPMRYLKSMRNPDFSEYREETGQGKEAALAAA
                     "
     gene            complement(14470..>14792)
                     /locus_tag="AC16UZ_11895"
     CDS             complement(14470..>14792)
                     /locus_tag="AC16UZ_11895"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6247430.1"
                     /translation="DDRKKDVVISGGENVYPAELENVLAEMPELAEYAVVGRPDERWG
                     EVPVACVVLRDGADLGEDELIARFAGRLARYKHPREVRFFQSLPRNVMGKVLKFRLRE
                     EVAP"
BASE COUNT         2691 a         4927 c         4795 g         2379 t
ORIGIN      
        1 cgccgcgcga acggcgggac gttcccccat gcgcccgagg tggtcgtgca cccggggata
       61 gccggacgag tcgatctgat ggaacttcag ccatcgggta atggtgaaga gatagggatc
      121 cgccacggtg aactgctcgc ccattaacca ggggccctcg aagaactgat cctcgatgag
      181 ctggaagcag tccgccatgt tgccaggcgc tttgcgcttc atcgcctcga tggcgctctc
      241 gtcgtccgcc cagcgctcgc cgcggcggct gtgggcgtgg gcaacatgca ccgtggcgca
      301 gaggtagctg gtgaacgcct gcagcctggc aagctcgaaa gggtcgtcca gcggggccaa
      361 tcgggcttcg ggatgggtct gggcgacata cagcaggatg gcgggcgtct ccgtcagcac
      421 gccccggtcc gtgacgagcg cggggacgcg gcccttggga ttcaccttcc tgtactccgg
      481 cgtgcgctgc tcgccggaca tgaaatccac caccttgagc tcgaagtcgg cgcccgcctc
      541 gcgaagtgtg atgtgcgagg ccagggcgca gctgcccggc atgaggtaga gcttcagcat
      601 ctgatctccc tgtgagccca caaccgcaga tgggatcttc actttagctc ggctgggggc
      661 tcacgtcgcg tccccgatac tgcggtctgc tagactcaaa ccgaagccac tgggaggctc
      721 cacaatgcag atcggcattc acgcgccgca cctcgggcgc gagctcgatc cggtgacggt
      781 tcgttcattc gtccaagagt gtgacaccct cggcgtccac tccctgtggg taagcgacca
      841 cgtctgctgg ccggcagaca tcgcctcgaa atatccctac accgaagacg gatcctttcc
      901 cgccacgccc gagatgggct ggctggaccc catcggaacg ctgctcttca ttgccggatg
      961 caccgaccgg gtacgtctgg gcaccacggt gctgatcctg ccctatcgcc cgccggtggc
     1021 cacagccaaa cagctggcca ccctggacgt tgtctcgggc ggccggctca ttctcggcgt
     1081 cggcatcggc tggatggccg aggaggccgc cgtcctgggc atgccctggg atcatcgcgg
     1141 acgccggtcc gacgagcagc tggagatatt caggcggctg ttcaccgaaa cggagccgag
     1201 cttcgaagga gagttttaca gcttccccaa ggtcggcttc gaaccgaaac cggtgcaaca
     1261 gccggttccg gtctgggtag ggggatccac gccggccgcg ttccggcggg tggcgcgatt
     1321 cggtcaggcc tttcacgccg cctttcagcc gctggccgag gtcggccggg aatggcgggc
     1381 ggtgcaggcg gcttgcgagg aaattgggcg cgatccctca gacctgagcc tgtctctgcg
     1441 gctctatctc gatccggaca gccgcatgga gccggaaaag tcggtggccg ggagcgcgga
     1501 gcagatgcgc gagaggcttt ttgagctggc cgctgccggc gtgggccacg tggtgctgga
     1561 tccggttgcc cgcgggggcg tgcaggggcg cctggacgcc gttaggggat tcatgaccga
     1621 cgtcgcccca gcgtcctgag gagcgaagcc ggccgtgcag gcccctgcgg aacggtttct
     1681 ccgtgagcac tgacgcgcca caataggccc atggactctg cgatgctcat cgacgtgctg
     1741 ctctggattc tggtcgtggg cctggtgatc gtcggcgtgg ccgggctgct gctgccggcc
     1801 ctgccggggg cacccctggt gttcgccgga cttctcatcg gcgcctggat cgacgatttc
     1861 gcctacgtga gctggggcac cctcatcact ctggggatca tggccgctct cacctacgcc
     1921 gtggatttcg ccgccggcgc cctgggcgca tcacgcttcg gcgcgtcgcc gcgggcggtc
     1981 tggggagccg tcatcggcgg catcgtgggc atcttcatgg gcctggttgg catcatcatc
     2041 gggcccttcg tcggcgccgt catcggcgag ctctccgcgc agcgctccct ggaggaggcc
     2101 ggcagggccg gactcggcgc caccctgggg ctcgtgatcg gcaccgccat caagctgacc
     2161 ctggcaatgg ccatggtggg catcttcgtc ctgatgcgct ttctctgacg gcgccgggca
     2221 ccttatcagg cagacgtcta cttctgcgcg tgcacgacga tggcgcggat ctgcatttgg
     2281 ctgcccagac gccgggcaag ggcgtccgcg acagctgcgt ggatcgcatc cgcatcgccg
     2341 ccgcgctgct cgatttcctg aaacagcggg ttaccgaaca ccaggcctcg cgcgaagttc
     2401 tccgccgagg gtatggcaga cgtcagctgg accgtctccg cggacgcggc ccggaagccg
     2461 gcgtcgagca gtgagcggcg aatgacctcg acgtcgtggt agccgaaggg cacgcggtag
     2521 aaaccggggg gatcgtcagg gaagaagccg gccaccacgt cgtgggtgat ctccgcgaag
     2581 gcgttgaacg gccaggcatc ccagacgttg aagacgtagt gaccgcccgc gcgcagcacc
     2641 cggcgggcct cggcgtagga acggtgtttg tcgggaaaaa acatgacccc gaactggcac
     2701 gtcaccacgt cgaagcgctc atcctgccac ggcaggtcgg tggcatcggc gacctcgaac
     2761 cccacgcttt cgtccggcgc aaacttcgcc tcagcgaaat ccagcatggg ctggttgagg
     2821 tcggacgcga cgatgtcgca acccgccgga aggacgtcgc ggagccgtcg ggtgacgatc
     2881 cccgtccctg ccgcgagctc gagaacggcg gcgggatcca gcgcggcaac ccgaccggcc
     2941 aggtcctcgg cgtagccttc gaagatgtgc ggacccaggc cggcgtcgta gtgctcgggt
     3001 atggagccga cgaaccggga agcttcttca gtcaaagctc acctccccgg accgtccgcc
     3061 gccgcccacc agcgcagtcg cctgccggac atggtagttg ccgagctcct ctttccagac
     3121 cagacgcagc tccccgaagt actcccgctt gatcttagtc agcggcgagg agccgttctc
     3181 gcgaaagtcg atccagctct tcatggcaac cctcccaatt ccagtctatc cctcgaacca
     3241 cacgcccgcc agaagcgcag cgcgagcccg cggcatacgc cgcgcacgag gtttgtcgcg
     3301 gtcgggagag cggcggtatg atccccgacg caggtaaagg gagacatcat gcgcgcattc
     3361 atctggatcg cattggtcgc gatcgctgct gtggtctact ttttcgcgac atccaccgct
     3421 cctgacgggg caacggagga tgggccgacc gcggccggcg gcggcactct ggaatctggg
     3481 ccgtccgtcg ggctgataga cgacgcgcgc atcaacgccg ccgacagcga gcccggaaac
     3541 tggctggcct acgggcgcac ctacgaggag cagcgcttct cgccgctgac tcagatcaac
     3601 cgggaaagcg tcggggagct gggcctcgcc tggtacaagg acatgggcac caaccgtgcg
     3661 ctggaggcca cgcccatcgt cgtggacggc atcatgttct tcaccaccac ctggagccgc
     3721 gtccacgcgg tggacgcacg gaccggggag acggtctggt tcttcgatcc cgaggtgccg
     3781 ggagagaccg gacgcaaagc ctgctgcgac gtggtcaacc gcggggtcgc cgtttatcag
     3841 ggcaaggtct tcgtcggcag cctggacggc cggctcatcg cgctggacgc ggccaccggc
     3901 gagaaggtat gggaagtgga caccatcgtc gatcgtaccc gggcctacac catcaccggc
     3961 gcgccgcgag cggcacgggg caagatcttc ataggcaacg gcggcgccga gtacggcgtg
     4021 cgcggctacg tcaccgccta cgatgcccag accggcgagc aggtctggcg cttctacacg
     4081 gtgcccggcg atccctccaa gcccttcgag catccggaga tggagatggc cgcgaagacc
     4141 tggaagggcg gcgagtggtg ggagatcggc ggtggcggca cggtgtggaa ctccatcgtc
     4201 tacgacccgg acttcaacca cgtctacctc ggcgtgggca acggttcccc ctggacgcgg
     4261 gtgatccgat caccgggcgg cggcgacaac ctgttcctct cctccatcgt cgccctgaac
     4321 gcggacaccg gcgagatgca gtggtactac cagaccaccc cgggcgacaa ctgggactac
     4381 acggccgttc aggacatcgc gcttgccgac atggaagtgg acggagagat gcgcaaggtg
     4441 cttctccagg cgcccaagaa cggcttcttc tacgtcatcg accggaccaa cggcgagctt
     4501 ctgcgcgcca atccctacgt cgcggtgaac tgggccaccc acgtggacat ggcaaccggc
     4561 cggccagtgg aaaatccggc catggattac gccgacacag cgaagtggat actcccggga
     4621 ccgctcggcg ggcacaactg gcaggccatg tccgtggacg tggaggccgg tttggtctac
     4681 atcccggcgc tggagaaccc gctgatctac ggcatgccgg atgagtggca gcagacgggc
     4741 gtctacaagc gcgacccggg ccgctggaac acggggatcg agctcggcgc catcgcgcag
     4801 ctgttcgtgg acaacatcgc cgagcagccc acgcctcgcg gtctgctcaa agccttcgac
     4861 ccgctctcag gcgaggagcg ctggtcggtg gagatcccac actactggaa cggcggcgtg
     4921 ctcggcaccg ccggcgggct ggtcttccag ggcaacgcca tggggatgtt caaggcctac
     4981 gacaaatcca ccggcgaggc gctgtgggag ttcaacactt acacctccat gctcgcacca
     5041 cccgtcactt tcgagctgga cggcgagcaa tacgtatcga tcctcaccgg gttcggcggc
     5101 ggcgacctgt tcggcggcga gcccctgccg ccgctgtcga tgcacgcttc gctgaagtac
     5161 aacaaccacg gccgtttgct ggtgttcaag cttggcgcca aagagcagct gccggttccc
     5221 tcggcccgtg accggaccat tccggagcag ctggtcgccg acgtcgacgt ggcggacatc
     5281 gcccggggcg agcgtctgta caacgactac tgcgccgtct gccacgggct ggtcgcacgc
     5341 tccagcgggg ccatcgccga tttgcgcctg atgtccgcgg agtcccacga cgccttcgac
     5401 ggggtcgttc tcgaggggct gatgtccgca aggggcatga cggcgttcag cgacgtgctc
     5461 acggtggagg attccgaacg cattcaccac tacgtcagag cccgggcaca cgaggaccgg
     5521 gaagtggcgc tcggaaactc ggaggctgcc aggctcacct ggctgaactg accgctaccc
     5581 gcccgggacc gccttgggcg gcagcgccgg cggcgggacc tgcacgaact cgaagtccgg
     5641 gcggcgccca acggcgcccg tcacggactg ctcggccgcg tagccggtgg tctcttcggg
     5701 acggccttcg ccctcgtcca ccacgcctcg gctcaaacgc ccattgagcc gctccccctc
     5761 ctccagcttt tcaagagcct cctgggccgc cctggcagac gcttcgtccc aggcaatccg
     5821 gtgcgccctg gggggggcga ccggaaaacc cgcctcgtcc agctcacgca cccagaaaaa
     5881 gatggtgccg ggggcgccgc tttccttgtc cggctcctcc accgtgatcc acaacacgcg
     5941 gaacgtctcc ggcagcgcag caggcgatgc ccagccgagc agaccctggt agccgagcca
     6001 ggccgacgcg taaagcgcag tgaccaacac caccgcaatt cccttgaccc acggcgagaa
     6061 gcgggtcgcc aggttcaggc acaggagcag ggcggccact gccacgtagg ccagggtgag
     6121 cccgagaatc gggccggtca cgtcttgccg cccactgccc gggtcaggag cggcttcggc
     6181 agctcgctgg tgctcgcgac ctgtccgtcg gcgccgatgg tgaaccgaac ggccgtctgc
     6241 tcgtccccca ccccggtgag ctgccgggtg ccgtagtaga cgagcgtaac cgtggggttg
     6301 agcttctcca ccttcacggt gacgggcagc ggctcgctgt agttcgactg atagtggagc
     6361 acgttcacca cgtactcacc cggctgcagc gcgcgaacgg tcaccgtctc ctgattgatc
     6421 gggttggaaa cctcccggcc gtccagaacg atccgatccg cgaacaggcc ccgatcgtcg
     6481 cggtccaggt gcatgagccc gctgtcgcga ttgtggtacc agaccagctg ccctcttggc
     6541 ccctcgacca gggtatcgac atcgtccggg tgccggtcgg cccacctcac ggtgatgagc
     6601 acctcggctt tcgagtcgat cttccccgtg ttgctcggat cgctgatcag ggcgaacgcg
     6661 gcgacgaaca tgaaggcgaa gcccagcagc gcgttgaaaa gcaggtcggt gaaagcgtcc
     6721 tgagattcgc gctgccggaa gacgtcatgc cgcatcgcgc ttcagcctca cgtccaccag
     6781 caccgcaagc cgcgtgacga gctgagcggc cgacgcatcg agcaggtgat attgcagctt
     6841 gagcagggtg ctggtgacca acccggccag cgtcgtgtag agcgctaccg ccatgccgcc
     6901 gctcatggca ctcagcagct gctgcatgag gctcgggtcg aactcttcta tctccccgac
     6961 gggcaggagc atgaggatga agccgaccac ggtgccgagc aggccgagct tcaacagaac
     7021 gtcgctggcg aaatgaccga acgcgtgccg gttcgccagc tcgtcgccaa acgcttcgag
     7081 cagagccgag gcgtcgtcat cgcgcttgag ggagaggttc tcgagaaacc cgccgagcag
     7141 accgcctctg acgtcgacgg cctcaccttc gcagatgctg ttctccagcg cgacgaagcg
     7201 ccgccgttcc gcacccagcg cgcgggccag ccacagccag tgaacgctca cagcgccgta
     7261 aagaccgaga atgaggtatg agatgcggct cttatcggag ccgagagcca ggctcaccag
     7321 gccctgctgc tcggccagaa aaaacccgaa cagcacgaga ccgaagatga cgagggcgcg
     7381 aaagacgagg ttggcgtctg tgagcttgaa catggcccta atccacgaac tgccgggttt
     7441 cagccccgtc gatatgccgg acgggcagct cgcaaatgct cgctggatca gccgcaagct
     7501 caaagctcac ggcaccaaac tgacagcttc ccccaatcac ggtgcccctc cccgccgacc
     7561 gatggactgc acaatgcgac caaggacgcc ggcaggtgtc aagaagccgc atccccgtcc
     7621 aaccccgacg gccgcatacg ctcccgccag cgaaggttgc aatctacata gcggcattcc
     7681 taaataggcc gcttttccgc gtgcagttgg aggtcgaccc atgagcaggt cagaagtcga
     7741 aggggcggag ttcgggttcg acccggaagc cctgcgagca aaatacgcag aggagcggga
     7801 caagcgcctg cgcgccgacg gcaacgcgca gtacttggaa gttaccggca agttcgccca
     7861 tttcctggac gatccctacg tggagcgcac accaagggac gccctgaccg acgaggtcga
     7921 agtcgccatc atcggcggcg ggtttggcgg cctgcttgcc ggcgcccggc ttcgggaagc
     7981 aggcgtcgag gatctgagga tcatcgagaa gggcggggat ttcgggggca cgtggtactg
     8041 gaaccgttac ccgggcgccc agtgcgacat cgagtcgtac atctatctcc cgctgctcga
     8101 ggaagtcggc tacatcccca gggagaagta cagcttcgcg ccggagatta tggcccacag
     8161 ccaggccatc ggccgcaagt acgaccttta ccgaaatgcc tgcttccaga ccgaagtcac
     8221 agagcttcgc tgggacgagg aggaccagcg gtgggagatc cgcactaacc gcggtgatcg
     8281 gatgaaggct cgtttcgtct gcatgtccaa cggtccgctc aaccggccga agctgcccgg
     8341 catcgaaggc atcgaatcgt tccagggcca cacgtttcac accagccgct gggactacgc
     8401 ctacaccggc ggcgacccga acggcaacct cgagaacctg gcgggcaaac gcatcggcat
     8461 catcggcacc ggagccaccg ccgttcagtg cgtgccgcat ctggccgaga cctctgaaca
     8521 ccttcacgtg ttccagcgaa cgccatcatc catcgatgcg cgcggcaacc ggcctaccga
     8581 cccgggctgg gcggcaaagc tcgcgccggg ctggcagcgg gaccgcatgc acaacttcaa
     8641 cgtgctcgtg accggcggct atcaggagga agatctggtc agcgatggct ggaccgacat
     8701 catccgcaac ctgggcatca tctccgcccg ccgcgctcag accgagagcc tgacgctcga
     8761 ggacatggca aagaacgtgg agctggccga cttccagaag atggagcaga tccgggcgcg
     8821 cgtggacgag cttgtcgacg atccggaaac ggctgaggct ctcaagccct actaccgaca
     8881 gttctgcaag agaccgtgct ttcacgacga gtacctgcag gcattcaacc gtccaaacgt
     8941 caccctggtg gacacggacg gaaggggcgt ggagcgcatc acggacaaag ggatcgtggc
     9001 aaacggcgtg gagtacgagc tcgactgcat catcttcgcg accggcttcg aggtaggcac
     9061 ctcgtacagc cgcagggcgg gctacgagat cgtcggccgg aacggcctga cgctgactga
     9121 caagtgggcc gacggcatga agaccttaca cggttttcag accaacggtt ttccgaactg
     9181 ctttttcatg ggcgtcacgc aaggcggttt taccgccaac tttccccatc ttctgaacga
     9241 gcagagcatc cacatcgctt acatgatcga tcaggcgaga aacaagaatg cccacgttct
     9301 ggaagcctcc cccgaagcgg aggcaggctg ggtagagacc attcggcaga tggcggtttt
     9361 caaccagaac tttctggaat cctgcacgcc cggctactac aacaacgagg gcaacccgga
     9421 ggtggcccga tcgctgctcg gcagccagta cggcggcggg ccggaggcgt tttttcagat
     9481 tctcagggac tggcgcgagg aggggggcat ggagggtctc gacttttcct gatgattctc
     9541 gcgccgggtg tcgaccatag agggagcgga catgaacgac tttgaaggca agacggcggt
     9601 ggtgaccggc gccgcgagcg gcatcggcct cgccctggcg gaacggttcg cggccgcgca
     9661 gatgcaggtg gtgctggcgg acatcgagga ggccgcgctg gaccaggcgg tcaaacgact
     9721 cgaagagcgg caggcgcgtg tgattggtgt ggtcaccaac accatggtgg agtcgtccgt
     9781 ggccgagctg gccgaacgcg cgctcgggga gttcggcaat gttcacgtgc tctgcaacaa
     9841 cgccggtgtg gcgagcctcg gcgccggggg ccggggcatc tgggaggtgc cggccgccga
     9901 ctgggactgg gtcatgggcg tgaacttcta cggggtgctc ttcggaatcc aggccttcgt
     9961 gccgcacatg ctcgagcatg gtgagccggg gcacatcgtc aacaccgcct cgctggccgg
    10021 cctctttccc ggcgggggca cctacggggt gagcaagcat gcggtgctcg ccctgacgga
    10081 aaccctgtac aacgatctca aggcacgggg cgcggcgctc agcgcctccg tcctctgccc
    10141 cggattcgtc agcacccgca tcgccaaggc cgagcgaaac cgtcccgcgg ggctcgagat
    10201 cgaccaaggc gacgtaagca gcgagatggc ggccgctgcc gaagccctga tcgcccaggg
    10261 aaaggatccc gccgagatcg cggaagccgt cttcgaggcc attgcccagg acaatctcta
    10321 cgtcctgccc catccggcat gggacgactt cgttcgcgcc cgggtcgatc acgtgctgga
    10381 cagacgcggc cccgcaacca tggacccggc cgagctcatg cgcagacgag cgggcggcga
    10441 ggccctctag ccggcttccg cggcgtccag cggttcgaac ccgagcagat cgcggccgtg
    10501 gtcgagatcg cggtatccat agcgatttcg agaattggcg aaaaaagtcc cgcaacgcac
    10561 cgcctccggc gcgcccaggg ccgcgacgag ggcgttgacc acgtcccgct ggctgcacca
    10621 gacgctgcgc tggcgggcgt tcgccggccg gtcctcggcg ttgacgaaac cgatgcggac
    10681 cgagatgaag gacgtggcgg tggtatcggc aaagtggcgg gcaagcgcct cgccccagac
    10741 cttggtgctg ccgtaaacgc cgcggggtcg cgggggcgcg tccaccgtga tcatcggcca
    10801 gcgatcgggc aggtcgtcat agcgcccctc gacgagcgcg cggtaaggct cgtcgtgctc
    10861 ccaaccggcc accgtggccc cgctggaggc gaaaacgacc cgcggcacgc cggcctccac
    10921 ggcggcctga aagacgttgc gcgttccctc cacgttggtg acgcgcaaag cgtcccagcc
    10981 gaagtgctca ccgggcttcg ccgccaggtg aatgacggcg tcgaccccgt cgaacgctgg
    11041 ccgaatggct tcgtagtcgg caatgtcggc ctgccaggtg aacgtgccgt tcacctcccg
    11101 gcggttcagc gccgcgacgt cgtggtcggc ggcaagaacc gggtgggcag cgccaccgat
    11161 gagcccgctc aagcccgtga cgagaagctt cacgcttcct cctttacggc ctgcacgagc
    11221 ttcggctccc aggtgaggta gggaaccggc tcgttctcga gaaaagctcg catcatggca
    11281 ttgaccacct cgggctgctc ctgctgaacc cagtgcgaga cccgcggcag gtaccgcagg
    11341 gtgaggttcg cgacgtagtc gtcggtgcca cgcgtggtct cgatgccgag cgcagcgtcc
    11401 tcctcgcccc agaccatgag ggtgggggtc tcgatgacgg gaaagccgag cgcgctctgg
    11461 cggcgaaaac cgccgccgcg caaggccgcc cggtaatagt tgatcatggc ttccagcgcg
    11521 ccgggctgcg ccgcgttgcg ccggtaaacg tccaccacct cgtccgggaa gcggctcgga
    11581 tccacggcga ccccccgaaa cgcatccgcc acggcccgtg cgcctcggcg acccagaaga
    11641 ttctcgggaa ggcggggaag ctgaaagaag aacacgtacc aggacttccg gagctgagcg
    11701 aacgatcgcc ccgatgctct gcctgccccg ggatgcggaa cgttacagat gatcagcttt
    11761 tccaggggac ggaccttgcg catggcgaag taccaggcca ccagggctcc ccagtcgtgg
    11821 gcgatgagca ccgtctcccg gcaaccggac gcatcgatca gccctgccac gtcgtccatg
    11881 aggttctcga tggcgtaagc gtccaccccg aagggccgcg acgtcaggcc gtaacccctc
    11941 aagttcggcg cccaggcccg aaagccgaga tctgccagca tgggcagctg atgacgccag
    12001 gagaagctgt gctccggaaa gccgtgcaga cacagcgcga ggcgccggcc ggagccacag
    12061 gcgtccacct cgaacctgag gccgttggca tcgacgaacg tcgtttcgat gcggggatcc
    12121 cgtattttcc gaggaacgat ctcatccacg aatgctgcct cccttcaact caaccgggca
    12181 cccggtgggc gccatcgcct ccagggccgt agatggccga gttccagata tcgccgagaa
    12241 cggcgccgat caccgccggc ccatcgggat tgtggcgggt catgttgtcg tttgccgcgg
    12301 cgttgctcat cagaatcagc gcccgggcca gtcggtccgg atcggcggcc ccgcttcgac
    12361 ccagcgcccg ctgccggcga atgaactccg ccgtgagatc gatgaagtaa tcgacgatcc
    12421 cccgctgcca ctcctgatag acctcctggc tcccgccggc cgcctgggca acggcggcaa
    12481 cgctgggcgc gtgccgttcg aacacctcga acatcgaggt gagatgggta agcgtcgcgg
    12541 cgcggtgatc gggctcatcc agttcccccg tgagccaggg atctaccgtg cggcgaatgt
    12601 ccgccgcgaa ctcctcgagc atacccagcg caatctcgtc caggccggaa aagtagtggt
    12661 agaaagagga acgcgtcatg cccgtccgct tcatcaagga atccacggtc agctcggaga
    12721 agcggacgtc ctgcagggcg gccatggccg ccgcgaggat ctccgcccgc gccgcctccg
    12781 gattgcgcct gacccgcgtg ctcttgacgt tcatgacctc cattcccgga atgcttcagt
    12841 cttccacccg gacattctac cccgggcaat ccactattga cacgatgtca atatataacc
    12901 tattttattg acgatgtgtc aataaaggcg ggggccgcat gaacgtactg gcatctcagc
    12961 tggaaccctg ggcggtggcc gatcgccagc cccgcatcgc catcatcggg gccggcatgt
    13021 ccggcatcgc cgccgtcgtc aagctccgca aagccggtta cacccacctc accgtttacg
    13081 agaaggccga ccgggtcggc gggacctggc gcgagaacac ctacccgggg ctgtcctgcg
    13141 acgtgccctc acgctggtac tcgttcagct ttgccctgag ctccgagtgg actcaccgat
    13201 tctcctacgg cccggagatc caggcctaca tggagaaggt cgccgagggc ttcggcgtga
    13261 cggacgtggt ccggttcgac accccggtga cggagctcac ctacgaagca ccgttctggc
    13321 gcctcactac cgccgcgggc gagcagcaga tctacgacgt cgtgatctcg gccacgggca
    13381 tcctccacca tcccgccatg ccggacatcg aaggactgga ggatttcgca ggagccagct
    13441 ttcactcggc ccgctgggat cactcggtgg atctcacggg caagcgtgtg ggcgtgatcg
    13501 gcaccgggtc cacggcagcc cagatcgtcg gcgacatcgc cggctccgtt ggcgagctcc
    13561 acgtattcca gagaacgccc cagtggatgg tgcccatgcc gcagaagacc tacagcgccc
    13621 gctggaagcg ggtgctcaag ctcttcccgt tcttgaagcg cctggcctat cagtactact
    13681 tccgcctcat ggttcgcacc ttcgccatgg ccaccgtggg cgatgagaag atgcaggccc
    13741 gcatcagcaa aatcacccgg cagaacctcg agcgcaacgt aaaggacccg gagctgcgcc
    13801 gcaggctgac gcccgactac caggcggcct gcaagcgcct catcctctgc tcggacttct
    13861 acccagccat cacccgcgac aacgcccacc tggtcacgga cgccatcgag cgcgtcaccc
    13921 ccgccggcat ccgaaccgcc gacggtcgcg tccacgagct cgacgttctc gtcctggcca
    13981 ccgggttcga tccgagcgcg ttcattctgc cgaccaaggt gacgggtgaa gacggggtgg
    14041 acctggagtc cttctggcag ggcgccccac gagcactgag agccgtggcg attccccgct
    14101 ttcccaactt ctggatgctg gagggcccta cgggccccgt gggcaacatg tccttgatca
    14161 ccatcaccga gcatcaggtg ggctacgtca tcgcgatgct cgagcgcatg aagaagcgcc
    14221 ggctcgtggc cgtctccgcg cgcgaggacg cctaccggaa ctacaaccag gccatgcagg
    14281 aggcggtgaa gaacacggtc tggatgacgg gcggctgcac gagctggtac atcgacaaga
    14341 ccgggatccc gaacctgtac ccctgggagc cgatgcgcta tctgaagtcc atgcgcaatc
    14401 ccgacttctc ggaataccgg gaggaaaccg ggcaggggaa ggaagcggcg ctggcagcgg
    14461 cctagagggt cagggggcga cctcctcgcg cagcctgaac ttgagcacct tacccatgac
    14521 gttgcgcggc agcgactgga agaagcgcac ttcccgggga tgcttgtaac gggcaagacg
    14581 tccggcgaag cgcgcgatca gctcatcttc acccaggtcg gccccgtccc gcagcaccac
    14641 gcaggcaacc gggacctcgc cccagcgctc gtccggccgg cccaccaccg cgtactctgc
    14701 gagctcgggc atctccgcca gcacgttctc gagctcggca gggtagacgt tctcaccgcc
    14761 ggagatgacc acgtccttct tccgatcgtc ca
//
LOCUS       DBNHOX010000541         1945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_750723, whole genome shotgun sequence.
ACCESSION   DBNHOX010000541 DBNHOX010000000
VERSION     DBNHOX010000541.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_750723"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="AC16UZ_11900"
     CDS             complement(<1..530)
                     /locus_tag="AC16UZ_11900"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR6247431.1"
                     /translation="MSWETEVGEIERRRHLARQQGGPEGIARQHAKGRLTIRERIGEL
                     LDEGSFREQGKATAIPDYDENDELTGFVPANYVVGFGQVEGRRVVVGGEDFTLKGGSP
                     NAAGLRKSVYAEHLASEYRVPLIRLLEGGGGSVKGSGRKGGTVGEPVYADPRFRIIAD
                     TMTEVPVASGAMGAVAG"
     gene            complement(543..1346)
                     /locus_tag="AC16UZ_11905"
     CDS             complement(543..1346)
                     /locus_tag="AC16UZ_11905"
                     /inference="COORDINATES: protein motif:HMM:NF005699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR6247432.1"
                     /translation="MSELVTIDVSGGVADVRLNRPEKYNALSPQMFDAIVAAGERLAQ
                     AKDVRAVVLSGNGRGFCAGLDMASFQGMTETSPRSSAGSLLQQDDRPENRAQRPALVW
                     KRLPVPVIAAIHGVAYGGGCQIALGADIRFAAPDAKMSIMEIKWGLIPDMSITQTLRD
                     VLPLDLAKELTFTGRVLSGAEASQLGLVTHVVEDPLAAALELAREIAGKSPHAVRAGK
                     KLFEESWHADARTGLELEAALQTGLIGSPNQVEAIKANFENRKPDFRDD"
     gene            complement(1403..1627)
                     /locus_tag="AC16UZ_11910"
     CDS             complement(1403..1627)
                     /locus_tag="AC16UZ_11910"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HIR6247433.1"
                     /translation="MARYEVESEVQGNVWKIEVGEGDRVSAGDVLIILESMKMEIPVE
                     APVSGVVAELRVREEEAVEEEQILLVLEAD"
     gene            complement(1637..>1945)
                     /locus_tag="AC16UZ_11915"
     CDS             complement(1637..>1945)
                     /locus_tag="AC16UZ_11915"
                     /inference="COORDINATES: protein motif:HMM:NF014804.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247434.1"
                     /translation="RAPRMHRVRVETGYQEGQAVTPFYDAMLAKVIGWGETRELAIGR
                     TLVGLKGFEIRGVKTNIPLLLDVLQDDGFLAGLIHTGFLDGLVLGSDLRSGSGVGRNA
                     "
BASE COUNT          328 a          708 c          554 g          355 t
ORIGIN      
        1 ccggcgaccg cccccatggc ccccgatgcc accggaacct cggtcatcgt gtcggcgatg
       61 attctgaagc gcgggtcggc gtacacgggt tcaccgacgg ttcctccttt acgacccgat
      121 cccttcacgc tgcccccgcc accctcgagg agccggatca gcggcacgcg gtactcgctg
      181 gcaaggtgct cggcgtagac gctcttgcgc aatccggccg cgttcggaga gccgcccttg
      241 agggtaaagt cctcgccgcc caccacaacg cggcgtccct cgacctggcc gaaacccacc
      301 acgtagttgg ccggaacgaa cccggtcagc tcgtcgttct cgtcgtagtc gggtatggcc
      361 gtcgccttgc cctgctcgcg aaaactgccc tcgtccagaa gctcgccgat ccgttcacga
      421 atggtgagcc ggcccttggc gtgctgacgt gcaatgccct ccggcccgcc ctgctggcgc
      481 gcaagatggc ggcggcgttc gatctcacca acctcggttt cccagctcat cgtgcggttc
      541 cgtcaatcgt ccctgaaatc gggcttcctg ttctcgaagt tcgccttgat ggcttcgacc
      601 tggttgggcg atccgatcag acccgtctgc agggccgcct ccagctccag ccccgtgcgc
      661 gcatccgcgt gccagctctc ctcgaacagc ttcttcccgg ctcgaacggc atggggcgac
      721 ttcccggcga tctcacgggc gagctccagg gcggcggcca gggggtcctc cacgacgtga
      781 gtcaccagcc cgagctgcga ggcttccgct ccgctgagca cacgcccggt aaaggtcagc
      841 tccttggcga gatctagagg cagaacgtca cgcagggtct gggtaatgct catgtccgga
      901 atgaggcccc atttgatctc catgatggac atctttgcat caggagccgc gaagcgaatg
      961 tcagcaccca gcgcgatctg acatccgccg ccgtaggcga cgccgtgtat ggcggcgatg
     1021 acgggcaccg gcagacgctt ccagacgaga gccggccgct gggcccggtt ttcgggtcgg
     1081 tcgtcttgct gcagcaggct gcccgcggat gaccgggggc tggtctcggt catgccctgg
     1141 aagctggcca tgtcgagacc ggcacagaaa ccgcggccgt tgccggacag aaccaccgcg
     1201 cgcacgtcct tagcctgtgc cagccgctct ccggctgcga cgatggcatc gaacatctgc
     1261 gggctcagcg cgttgtactt ttccggccgg ttgagacgca cgtcggccac gccgcccgat
     1321 acgtcgatgg tgaccagttc cgacatagtt tccccccaac ccagaagacg gccctctaac
     1381 ttaacacaat tgctcgtccg ccctagtccg cctcgagcac cagcagaatc tgttcctctt
     1441 caacagcctc ctcctcgcgc acgcgcagct ccgcgacgac gccggagaca ggcgcctcca
     1501 ccggaatctc cattttcatg gactccagaa tgatcagcac gtctcccgcc gacacccggt
     1561 cgccttcgcc gacctcgatc ttccagacgt taccctgaac ctcactttcg acctcgtagc
     1621 gagccacgtt acctcctcat gcgttccggc cgactccgct cccgctccgc agatcggagc
     1681 caagcacaag cccatcgaga aatccggtgt ggataagccc ggcaagaaac ccgtcgtcct
     1741 gcagcacgtc gagcagcagc ggtatgtttg tcttgacccc ccggatctcg aatcccttga
     1801 gcccaaccag cgttcggccg atggcaagct cgcgggtctc gccccagccg ataacctttg
     1861 ccagcatggc atcgtagaac ggcgtaacgg cctgaccctc ctggtaaccc gtctccaccc
     1921 gtacccggtg catccgcggc gcccg
//
LOCUS       DBNHOX010000542         3564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1457130, whole genome shotgun sequence.
ACCESSION   DBNHOX010000542 DBNHOX010000000
VERSION     DBNHOX010000542.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1457130"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="AC16UZ_11920"
     CDS             <1..672
                     /locus_tag="AC16UZ_11920"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6247435.1"
                     /translation="RGDAFPSWQGDLFVGSLADQSIRRLRIRGTTLVHQERLLPDLNE
                     RIRSLATGPDGFLYALTDSSNGKILRVRPGRPAPHELARVAQPFEMTEGADIFEKLKE
                     HGVMQSEETSLADQAAYDAARAETLFVQTCGSCHVFEDAGSGNIGPKLDGVVGRRSGT
                     LPGFSYSEALANSETSVVWGYFTLTAFLTNPQAYYPGNRMAAAPLRYEDALQISMYLN
                     HGETF"
     gene            827..2347
                     /locus_tag="AC16UZ_11925"
     CDS             827..2347
                     /locus_tag="AC16UZ_11925"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0032787 - monocarboxylic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6247436.1"
                     /translation="MSVKAYDWVAYHARQTPEKLALVDLHSNRQFSYRDMQERTARLA
                     GAMRDGFGVRPGDRVAALLNNSSDCMEMNFACMKLGAIYVPLNWRLTVSELEYIVGDA
                     EPSLLIHEDVFSDEAEALKERCSVRALLETTASGTGNAYEAAIDAAAPLTGSAEITHD
                     DTWVIMYTSGTTGHPKGCMITYGMTFWNVVNLLNPHRLSEDMVNLITLPLFHTGGLNV
                     YGNPAVHMGGTNIVMRSFDPGETLRILQDAEYGITHMIGVPANYLFMSQHPAFAEADL
                     SRLRAMAVGGAPTSLELLKIYADKGNALQQAFGMTETSPLVTALTPANALDKPGSAGL
                     AVLHTEIRLVDEEAREITAPNVVGELWVKGPNVTPGYWRRPDANESSFTDGWLHTGDA
                     AYQDEDGFIYIVDRWKDMYISGGENVYPAEVENVIYQLPAVAEVAVIGVPHERWGETG
                     QAVIVLKEGESLSEEAVLKHCKDKLARFKQPSSVRFIDEIPHNATGKQLKRELREQ"
     gene            2423..2782
                     /locus_tag="AC16UZ_11930"
     CDS             2423..2782
                     /locus_tag="AC16UZ_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247437.1"
                     /translation="MAWHSAGCRVHRGAGATLYLQNLLNFDWEQTRSPAGAIIRLSTD
                     GSLRQSVPDAHVVGKFNPPVMTTADLALKFDPEYRKVAERFLADPDACQRQLKYTATP
                     VDLIFGSTAELRAIAAV"
     gene            complement(2873..>3564)
                     /locus_tag="AC16UZ_11935"
     CDS             complement(2873..>3564)
                     /locus_tag="AC16UZ_11935"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6247438.1"
                     /translation="GDQAAGCSDEDARHIVAAMARLHAQFWASETLDEKSWITPYAVT
                     SRIMQMRFLQGVDQYLCDEGGSLTPARRDLIGWLRKNGIALTETFGDEPATLLHGDVR
                     LDNICFDDATGEVVLFDWQTILAGPGASDLAYFISATLPGDADESQVDELIDVYHGEL
                     SQHGVDVSRDRLRWQYEVGMLTMLHRVLPTTYPDQMELDAGRGLPLLQAWISRIYNRL
                     EQIDHERILKI"
BASE COUNT          702 a         1122 c         1129 g          611 t
ORIGIN      
        1 cgcggcgacg cctttcccag ctggcagggc gatctcttcg tcggctctct cgccgatcag
       61 tccatcaggc gcctgagaat tcgcggtacc acgctcgtgc accaggaacg actgctgccc
      121 gacctcaacg aacgcatccg ctccctggcc acaggacccg acggctttct ctacgcgttg
      181 accgattctt ccaacggcaa gattctccga gtccgtccgg gacgaccggc gccacatgag
      241 ctggcccggg tagctcagcc tttcgagatg accgaaggcg cagacatatt cgaaaagcta
      301 aaggaacacg gcgtcatgca gagcgaggag accagcctgg cggatcaggc cgcttacgac
      361 gcggcgcgcg cagaaaccct gttcgtgcag acttgcggaa gctgccatgt gttcgaggac
      421 gctggcagcg gcaacatcgg cccgaagctg gatggcgtcg tgggccgccg atccggcacg
      481 ctgcccggat tttcgtattc cgaggccttg gccaacagcg aaacgagcgt cgtctgggga
      541 tacttcaccc ttactgcctt cctgaccaac ccgcaggcct actatcctgg caacaggatg
      601 gcggcggcgc cgctgcgcta cgaagacgcg ctgcagatca gcatgtacct gaaccatggg
      661 gaaaccttct agctgacatc gacgcgtccg actggccagg tccccgggcc gtttgggtga
      721 cgttgccgcg ggcagcgaac ccgttcgcgg ctcctgcggc ctgcggggcg caggctgcaa
      781 gccatcgtct atactcccca tcatcgagag ggaggggagc ccgcagatgt ccgtgaaagc
      841 ctatgactgg gtcgcctatc acgcccggca gacgccggag aagctggcgc tggtcgacct
      901 gcattcgaat cggcaattct cttatcgcga catgcaggaa cgaacggcga gactcgcggg
      961 ggcgatgcgc gacggtttcg gcgttcggcc cggtgaccgc gtggcggcgc tgctgaacaa
     1021 ctcttcggac tgcatggaga tgaacttcgc ctgcatgaag ctcggagcca tctacgtgcc
     1081 tctcaactgg cggctgacgg tgtccgagct ggagtacatc gttggcgacg ccgagccgag
     1141 cctgttgatc cacgaggatg ttttcagcga cgaggccgaa gcgctcaagg agcgatgcag
     1201 cgttcgcgcc ttgctcgaaa ccaccgcaag cggaaccggc aacgcttacg aggcggcgat
     1261 cgacgcggcc gcaccgctga ccgggagcgc ggagatcacc cacgacgaca cctgggtgat
     1321 catgtacacc tccggaacca ccggccaccc caagggctgc atgatcactt acggcatgac
     1381 gttctggaac gtggtgaacc tgttgaaccc ccatcggctg tcagaggaca tggtgaacct
     1441 gataacgctg ccgctgtttc acacgggggg gttgaacgtc tacggaaatc cggccgtgca
     1501 catgggaggc accaacatcg tgatgcgctc gttcgacccg ggcgagaccc tgcgcatcct
     1561 ccaggatgcc gaatacggca ttacccacat gatcggcgtg cctgccaact atctgttcat
     1621 gagccagcat ccggcatttg ccgaagccga cctctcccga cttcgtgcca tggcggtagg
     1681 cggggcgccc acgtcactgg agctcctgaa gatctacgcc gacaagggca acgcgttgca
     1741 gcaggcgttc ggcatgaccg aaaccagccc cctggtgacc gcgctgacgc ccgccaacgc
     1801 gctggacaag ccaggctcgg cagggcttgc ggtgctgcat accgagatcc gtctggtcga
     1861 cgaggaggcc cgggagatca ccgcgccgaa cgtggtcggg gagctgtggg tgaaaggccc
     1921 gaacgtgacg cccggttact ggcgccgacc ggacgcgaac gaaagctcgt tcacagacgg
     1981 ctggctgcac acgggggatg cagcctatca ggacgaggat gggttcatct acatcgtgga
     2041 ccgctggaag gacatgtaca tctcaggggg tgaaaacgtc tatccggcgg aggtggagaa
     2101 cgtcatctac cagctgccgg cggtggccga ggtggccgtg atcggggtgc cccacgagcg
     2161 ctggggcgag actggccagg ccgtgatcgt gctgaaagag ggagaaagcc tgtcggaaga
     2221 agccgtgctg aagcactgca aggacaagct ggcacggttc aagcagccga gctcggtgcg
     2281 cttcatcgac gagatcccgc acaacgccac cggcaagcag ctcaaacgcg agctcagaga
     2341 gcaatgagcc ctcggccagc cgcgttcaac tcagttctgg tggccggccg agttgggtaa
     2401 ctaaggtccg ctgttcatcc gcatggcatg gcacagcgcc gggtgccgcg tccatcgggg
     2461 agcaggggcg acgctgtacc tgcagaacct gctgaacttc gactgggagc agacccgcag
     2521 ccctgcgggc gccatcatcc ggctctccac ggacggatcg ctacgccagt ccgtgccgga
     2581 cgcccacgtc gtgggcaagt tcaacccgcc cgtaatgacc accgccgatc tcgcgctgaa
     2641 gttcgatccg gagtacagga aagtcgcgga gcggttcctg gctgatccgg atgcgtgcca
     2701 gcggcaactc aagtacaccg ccacgccagt ggatctgatc ttcggctcca ccgccgagct
     2761 gagggcaatt gccgcggtct aggcctacga cgacgggaag gaacttttcc tgagcgactt
     2821 cgtcgccgcc tgggtcaatg gcatgcggct ggaccgcttc gacctgtagg cttcagatct
     2881 taagaatacg ctcgtggtca atctgctcga gccggttgta gatccgcgag atccaggcct
     2941 gcagcaacgg caagccgcgc ccggcatcga gttccatctg gtccggatag gtggtgggga
     3001 gcacccgatg cagcatcgtc agcatgccca cctcgtactg ccagcgcagc cgatcccgtg
     3061 agacgtctac cccgtgttgc gaaagctcac cgtggtagac gtcgatgagc tcgtcgacct
     3121 gagattcatc ggcgtccccg gggagcgtcg cgctgatgaa gtaggcaagg tctgaagcgc
     3181 ctggaccggc caggatcgtc tgccagtcaa acagcacgac ctcgccggtg gcatcatcga
     3241 aacagatgtt gtccaggcga acgtctccgt gcaggagggt ggctggttca tcaccgaacg
     3301 tctccgtgag cgcgatgccg ttctttcgaa gccagccgat gagatcccgg cgtgccggcg
     3361 tcagactccc gccctcatcg cagaggtact gatcaacacc ctgcagaaaa cgcatctgca
     3421 tgatcctgct ggtgacggcg tatggcgtga tccagctctt ttcatcgagc gtttcgctgg
     3481 cccagaactg ggcgtggagg cgagccatgg ctgcaacgat gtggcgagca tcctcgtcag
     3541 agcaaccagc agcctgatct ccca
//
LOCUS       DBNHOX010000543        10241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_623336, whole genome shotgun sequence.
ACCESSION   DBNHOX010000543 DBNHOX010000000
VERSION     DBNHOX010000543.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10241
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_623336"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="AC16UZ_11940"
     CDS             <1..738
                     /locus_tag="AC16UZ_11940"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HIR6247439.1"
                     /translation="SAARHVEEGAQVIDINFGCPVKKVCNKAAGSALLKEPDLVVAIV
                     RAAVAAVPVPISVKMRTGWSPDSRNAVELAQRMEDEGVAAIAVHGRTRACRFNGEAEH
                     GTVAAIKSRVSIPVFANGDIDSPGSARRILEETGVDGVMIGRAALGRPWLLGEVADGV
                     PRVASLGEKLAVVAEHVAALHEFYGEPGTRIARKHVQWYLQHLSQLGPREVRSRLGKA
                     FNRLEQAQAQLDFLAELPASAIGRRAA"
     gene            738..836
                     /locus_tag="AC16UZ_11945"
     CDS             738..836
                     /locus_tag="AC16UZ_11945"
                     /inference="COORDINATES: protein motif:HMM:NF014950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6247440.1"
                     /translation="MARIGGNKSRAATMLGMNRGTLRKKLEGYGII"
     gene            833..2389
                     /gene="purH"
                     /locus_tag="AC16UZ_11950"
     CDS             833..2389
                     /gene="purH"
                     /locus_tag="AC16UZ_11950"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR6247441.1"
                     /translation="MSNADARSVARALLSVSDKTGLEEFARGLSALGVEILSTGGTYR
                     ALSEAGIRVTEVSAHTGFPEMMDGRVKTLHPKIHGGILGRRDVDGATMTEHDIEPIDL
                     VVVNLYPFEATIERADCTDELAIENIDIGGPAMVRSAAKNHRDVLVVVDPGDYGHLLE
                     TLQRDGATTLESRRALAAKAFRHTARYDALVAGYLTAEQEFPESLTLSYRHVETMRYG
                     ENPHQQAAFYRESGARPGTVAALRQLQGKSLSYNNVADTDAALECVKVFERPACVIVK
                     HANPCGVAEAADVQAAYERAFATDPTSAFGGIIAFNRPLDEPTLTLILERQFVEVIVA
                     PEAAPEAVAAAAARKNVRLLVTGPLQAGSRGLELKRVGGGLLVQTPDETCLGEEGLRV
                     VSRRAPSASELDDLLFAWKVAWFVKSNAIVYARDGQTIGVGAGQMSRVVSARIAGFKA
                     AEEGLEVAGSVMASDAFFPFRDGIDAAAEAGIAAVVQPGGSVRDDEVIAAADEHGMAM
                     VFTGIRHFRH"
     gene            2426..3706
                     /gene="purD"
                     /locus_tag="AC16UZ_11955"
     CDS             2426..3706
                     /gene="purD"
                     /locus_tag="AC16UZ_11955"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009041976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR6247442.1"
                     /translation="MNVLVIGSGGREHALAWKAAQSDGVETVFVAPGNAGTATEAGCE
                     NVDIAVDDFPALAEFAAANGVGLTIVGPEVPLVEGVTDYFEGRGLACFGPSKMAAQLE
                     GSKAFTKAFLDRHRIPTARYQAFTDLAAAQAYVREQGVPIVIKADGLAAGKGVVVAET
                     IEQALQALDDMLSGSAFGEAGSRVVIEEFLAGEEASFICISDGTNVVPFATSQDHKAR
                     DDGDKGPNTGGMGAYSPAPVVTAGVYDRIMKEVVNPTVRGMAEDGVPYVGFLYAGLMI
                     MPDGAPRVIEFNCRFGDPEAQPVMMRLRSDLVGLCRQALDGELAGVELDWDPRVALGV
                     VMAAGGYPEAYDKGDAITGIDTADADPRVKTFHAGTRLEDGVLVTNGGRVLCVVGLGD
                     DVAGARAAAYGAVEHIDWRNRYYRRDIGHRALDR"
     gene            3778..4746
                     /locus_tag="AC16UZ_11960"
     CDS             3778..4746
                     /locus_tag="AC16UZ_11960"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR6247443.1"
                     /translation="MIVRSLRRFEPFARLPSAALIPVAEHASTLILPAGRWLVRPGRS
                     LPGHYYLQRGAVRLREPDQRVAAGSPRSRWPIYPGAQGVLTLSPVTLVRVSEDAAAAT
                     AHAREVQPPLSRPQWLTDGWECRFLSSGAMGRVSLDHCQRVMRAMRPVDLLRGEMVVR
                     TGEPGDTVFVLAAGRAEVSRGRRSLALLEPGDHFGEDAAISGACRNACVTMVSDGRVM
                     TLSGELFRDFVVGRVLLDDGDGPAQATIDIGGRSVSAGLGIPLLLLRERLGLLTPELR
                     YRLAGGSLRQRVLAAFILLHRGFRVALAESGLDAEVALHPEPRAHL"
     gene            4821..5165
                     /locus_tag="AC16UZ_11965"
     CDS             4821..5165
                     /locus_tag="AC16UZ_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR6247444.1"
                     /translation="MSSDCLFCRIASGEIPGDIVYEDDELLAFNDISPQAPHHVLVIP
                     KIHVATVNDLAAEHADLVGRLVLCARAIAAERNIQESGYRLILNCNGHGGQTVFHLHL
                     HLLGGRQLMALG"
     gene            5167..5805
                     /gene="coq7"
                     /locus_tag="AC16UZ_11970"
     CDS             5167..5805
                     /gene="coq7"
                     /locus_tag="AC16UZ_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HIR6247445.1"
                     /translation="MAGPGSLADALVAQVDKALRTLTGAAASVRENPAADVPEGEMSP
                     GEREHAAGLMRVNHTGEICAQALYEGQALTASDPRTRTALLAAASEESDHLAWCAGRL
                     AELGSSPSVLNPAFYALSYALGAVTGVLGDKVSLGFVEATEDQVCRHLESHLQSLPAG
                     DSRSRQIVRAMREDEARHGAEALGAGGAEFPRPVKRAMSLASRLMTETTYRV"
     gene            complement(5793..6206)
                     /locus_tag="AC16UZ_11975"
     CDS             complement(5793..6206)
                     /locus_tag="AC16UZ_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012274236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6247446.1"
                     /translation="MTKPPKPHAGLRHVALFTTTLEASVRFYTELLGMQVEWNPDPDN
                     FYLTSGTDNLALHRATEGRSVRGQTLDHIGFIIDDEEDVDAWHDHLAANGVNILERPR
                     THRDGARSFYCEDPDGNVVQLIYHPPISRPGGQTR"
     gene            complement(6226..6645)
                     /locus_tag="AC16UZ_11980"
     CDS             complement(6226..6645)
                     /locus_tag="AC16UZ_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014872825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR6247447.1"
                     /translation="MQAKITLIENVHFEAVADSGHTVLIDGPPDAGGENRGTRPMELV
                     LMGLGGCASFDVVSILRKSRQPVVGCVTRLTARRADTVPQVFEAIHLHFTISGQGLDE
                     AKVARAVALSAEKYCSASIMLERGGVAISHDYEILEA"
     gene            6845..7504
                     /gene="crp"
                     /locus_tag="AC16UZ_11985"
     CDS             6845..7504
                     /gene="crp"
                     /locus_tag="AC16UZ_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HIR6247448.1"
                     /translation="MGKPLTAKGFNPNAGQEDTHLDAFLAAAHRRKYPAKSTLIYAGD
                     KSDSIYYILKGSVTVLVEDESGREIIVAYLNKGDFFGEMPLFTESAPRSAWIKAKTEC
                     EVAELSYGKFRELAQRHSDLLYAVGRQMADRLGRTTQKVSDLAFLDVTGRVARTLIEL
                     TKQPDAMTHPDGMQIKITRQEIGRIVGCSREMVGRVLKTLVEQGLVNVKGKTMVVYGT
                     R"
     gene            complement(7649..8434)
                     /gene="trpC"
                     /locus_tag="AC16UZ_11990"
     CDS             complement(7649..8434)
                     /gene="trpC"
                     /locus_tag="AC16UZ_11990"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR6247449.1"
                     /translation="MTILDEILAHKRTEVAERQRVRSLDSLRALAQACPPARGFADAL
                     ESKLLAGASGVIAEIKRASPSKGVIREDFDPVSIARSYAKGGAACLSVLTDERYFQGH
                     DEYLQAARAAVDLPVLRKDFVVDAYQLFEARAMGADCVLLIAAALDAGQLKALHETAI
                     ALGLDVLVEVHDGEELESALEIEPRLVGINNRNLKTFETRLETTYGLLPGIPDSVTVV
                     TESGIANREDVAAMRQHGVHCFLVGEAFMRAPDPGTALAEMFA"
     gene            complement(8482..9504)
                     /gene="trpD"
                     /locus_tag="AC16UZ_11995"
     CDS             complement(8482..9504)
                     /gene="trpD"
                     /locus_tag="AC16UZ_11995"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR6247450.1"
                     /translation="MDIQGALAALFEKRDLSLEDARAVFVEVMSGHATPGQIGALLAA
                     LRVKGETPQEIAGAAEAMRSLSTKVVVDVPYLVDTCGTGGSGAKLFNVSTAATFVAAA
                     AGAHVAKHGNRKMTSFSGSADVLEAAGVNISLAPEQIAHCIREVGVGFLFAQAHHSAM
                     RHAAPVRQELGARTIMNVLGPLTNPAGARNQVLGVFSAAWQTTLAEVLKLLGASHVLV
                     VHSNGLDELALNGPSFVVELNAGTITEYEIEPADFGLKPSSHDELRADSPETSLALLK
                     RSLTEPDSSAADIVSLNAGAAIYAAGVATSFKNGVVMAQDAIAAGLASERLAELVRVS
                     SMMAEP"
     gene            complement(9510..10085)
                     /locus_tag="AC16UZ_12000"
     CDS             complement(9510..10085)
                     /locus_tag="AC16UZ_12000"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019472944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR6247451.1"
                     /translation="MLLMIDNYDSFTYNVVQYLSELGAEVAVYRNDEITLTEIEELAP
                     EKIVISPGPCTPDEAGVSMEVVTRFGPTIPILGICLGHQSIGQAMGGTVRRAREVMHG
                     KTSLIHHDDRGVFHGLPRPFEATRYHSLVVAHEDLPDCLEVTAWTERDGELDEIMGLK
                     HREYPIEGVQFHPESIRTPVGHDLLRNFLEG"
BASE COUNT         1672 a         3278 c         3507 g         1784 t
ORIGIN      
        1 agtgccgcgc ggcacgtcga agaaggtgcc caggtgatcg acatcaactt cggctgtccg
       61 gtcaagaagg tctgcaacaa ggctgcagga tcggctctgc tcaaggagcc ggatctggtt
      121 gttgccatcg ttcgcgctgc ggttgccgcc gtgccggttc cgatctcggt gaagatgcgc
      181 acgggttggt cgccggattc ccgtaacgcc gtggagctcg cgcagcggat ggaggacgag
      241 ggcgtcgcag ccatcgccgt gcacggccgc acgcgcgcct gccgcttcaa cggcgaggcg
      301 gagcacggca ccgtggccgc catcaagtcc agggtgtcca tcccggtatt tgccaacggc
      361 gacatagact cccctgggtc ggcgcgccgg atactcgagg agaccggcgt cgacggcgtc
      421 atgatcggcc gtgctgccct gggcagacca tggttgctcg gcgaggtagc ggacggggtg
      481 cccagggtgg cttcgctggg tgagaagctc gccgtcgtgg cggagcatgt cgccgccctg
      541 cacgagttct acggtgagcc cggcacccgc attgcgcgaa agcacgttca gtggtacctc
      601 cagcacctga gccagctggg gccgagggag gtccgctccc gtctcggaaa ggcgttcaat
      661 cgactggagc aagcgcaggc ccagctggat ttcctggctg aactgccggc ctctgccatc
      721 ggtcggagag cggcgtgatg gcgcgcatcg gcggcaataa aagtcgcgcg gcgaccatgc
      781 tcggtatgaa tcggggcacg ctgcgcaaga aactcgaggg ttacggaatc atatgagcaa
      841 cgctgacgcg cggagtgtgg cccgcgccct gctgagtgtg tcggacaaga cggggctcga
      901 ggagttcgcc cgcgggctct cggctctggg cgtggagatc ctctcaacgg ggggtaccta
      961 ccgggcgctc tcggaagcgg gcatcagggt cacggaggtc tccgcccaca ccggtttccc
     1021 ggaaatgatg gatggccgcg tcaagacgct gcacccgaag atacacggcg gcatcctcgg
     1081 ccggcgcgac gtggatggcg cgaccatgac cgagcacgac atcgagccca tcgatctcgt
     1141 ggtcgtcaac ctctacccgt tcgaggccac catcgagcgc gccgactgca ccgacgagct
     1201 ggccatcgag aacatcgaca tcggcgggcc cgcgatggtc cgctcggcag ctaagaacca
     1261 tcgcgacgtt ctcgtggtgg tagatcccgg ggattacggc catctgcttg agaccctgca
     1321 gcgcgacggc gcgaccacgt tggagagccg gcgcgcgctg gccgcgaagg ccttccgcca
     1381 tacggcccgc tacgatgcgc tcgtggccgg ttacctcacc gcggagcagg agttcccgga
     1441 gtcgctcacg ctcagctatc gccacgtgga gaccatgcgg tacggggaga atcctcatca
     1501 gcaggcggcc ttttaccgcg agtcgggggc gcgaccgggc accgtggccg ccctgcgcca
     1561 gctgcaaggc aagagcctgt cctacaacaa cgtggccgat accgatgccg cgctggagtg
     1621 cgtcaaggtg ttcgagcggc cggcctgcgt catcgtcaag catgcaaacc cgtgcggcgt
     1681 tgcggaggcc gccgacgtac aggccgcata cgaacgtgcc ttcgcgacgg atccgacctc
     1741 ggcgttcggt ggcatcatcg ccttcaacag accgctcgac gagcccacgt tgacgctgat
     1801 tctcgagcgt cagttcgtgg aggtcatcgt cgcgcccgag gcggcgccgg aggcggtggc
     1861 cgctgcggcg gcccggaaaa acgttcgtct tcttgtcacc ggccctttgc aggcaggctc
     1921 ccgcgggctc gagctgaagc gcgtgggtgg cgggttgctc gtccagacgc ccgacgagac
     1981 ctgcctgggt gaagaggggc tgcgggtggt gtcgcggcgc gcgccgtctg ccagcgagct
     2041 cgacgacctg ctgttcgcct ggaaggtggc atggttcgtc aagtccaacg ccatcgtcta
     2101 cgcccgcgac ggccagacca tcggcgttgg cgcgggtcag atgagcaggg tggtgagcgc
     2161 ccgaatcgcg ggattcaagg cagccgagga ggggctggag gtagccggct ccgtgatggc
     2221 ttccgatgcc ttctttccct tccgggacgg catcgacgcg gctgccgagg ccgggatcgc
     2281 ggcggtggtt cagccgggcg ggtccgttcg cgacgacgag gtcatcgcgg cggccgacga
     2341 gcacggcatg gccatggtgt tcaccggcat acgtcacttc cggcattagc ctttgcgggg
     2401 ccatcgaacc ttagaggatg cctagatgaa tgtgcttgtc attggcagcg gcggcaggga
     2461 gcacgccctg gcctggaagg ccgctcagag cgacggcgtg gagacggtct tcgtggcacc
     2521 gggaaacgcg ggaacggcga cggaggccgg gtgcgagaac gtcgacatcg cggtggacga
     2581 tttccccgcc ctggcagagt ttgccgccgc gaacggcgta gggctcacca tcgtcgggcc
     2641 ggaagtcccc ctcgtggaag gcgttaccga ttacttcgag ggccgcgggc tcgcctgctt
     2701 cgggccgagc aagatggcgg cccagctgga aggctccaag gctttcacca aggcgtttct
     2761 ggaccgtcac cggatcccga ccgcgcgcta ccaagccttc accgatctgg cggccgctca
     2821 ggcctacgtt cgcgagcagg gcgtgcccat cgtcatcaag gcggatggcc tggcggccgg
     2881 aaagggggtg gtggtcgcgg aaaccatcga gcaggccctt caggcgctgg acgacatgct
     2941 cagcggatcg gcttttggcg aggccggatc ccgggtggtg atcgaggagt tcctggctgg
     3001 cgaggaggcg agcttcatct gcatatcgga cggcaccaac gtggtgccgt tcgccacgtc
     3061 tcaggatcac aaagcccggg acgatgggga taaggggccc aacacgggcg gcatgggcgc
     3121 ctactcgcct gcccccgtgg taactgccgg cgtctatgac cgcatcatga aggaggtggt
     3181 caaccccacg gtgcgtggca tggcggagga cggcgttccc tacgtcggct tcctctacgc
     3241 cgggctcatg atcatgcccg acggtgcgcc cagggtcatc gagttcaact gccggttcgg
     3301 cgatccggaa gcccagcccg tgatgatgcg cctgcgctcc gatctggtcg gtctctgccg
     3361 gcaggcgctg gatggggaac tcgccggcgt cgagctcgac tgggacccgc gggtggcgct
     3421 cggcgtcgtg atggctgcgg gggggtaccc cgaggcgtac gacaagggtg acgccattac
     3481 cggcatcgat accgcagacg cagatccgcg cgtgaagacc tttcatgccg gcacccgtct
     3541 cgaagacggc gtgctggtga ccaacggtgg ccgcgttctc tgcgtcgtgg ggcttgggga
     3601 cgacgttgcc ggcgcccgtg cggccgcgta cggagccgtc gagcacatcg actggcggaa
     3661 ccgctactac cggcgcgaca tcgggcatcg agcgctcgac agataacggc aatttccgtc
     3721 acgccaacaa tctgaaggcg gcggcgccgc agctccgcgc cgcgggaaaa tggcgccatg
     3781 attgtccggt cgctgcgccg cttcgaacca tttgctcgac tcccgagcgc ggcgctcatt
     3841 ccggtggccg agcacgccag caccttgatc ttgcccgcgg gccgttggct ggtcaggcca
     3901 ggccggtcgc tgcccgggca ctactatctg cagcgaggag cggttcgttt gcgggaacct
     3961 gaccaacggg ttgctgccgg gtcgccgcga tcccggtggc ccatctaccc gggcgctcag
     4021 ggcgtgctca ccctatctcc ggtcaccctc gtgcgcgtga gcgaagatgc ggcggccgcc
     4081 acggcccacg cccgggaagt ccagccgccg ctctcccggc cccagtggtt gaccgacggg
     4141 tgggagtgcc gctttctcag ctccggagcc atggggcggg tcagcctgga ccactgtcag
     4201 agggtgatgc gggccatgcg ccccgtggac ctcctccggg gcgagatggt ggtgcgaacc
     4261 ggggagcccg gcgataccgt cttcgtgctt gcggccggcc gagccgaggt cagcagaggg
     4321 cggcgttcgc ttgcgctgct cgagccgggt gaccatttcg gggaggatgc ggccatcagc
     4381 ggtgcttgcc gcaacgcttg cgtgaccatg gtctccgacg gtcgcgtgat gacgctctcc
     4441 ggtgagctgt ttcgcgactt cgtggtcggg cgggtgcttc tcgacgacgg cgatggcccc
     4501 gcccaggcga ccatcgacat aggcggacgg tccgtgtcgg cgggcctggg tatacccctc
     4561 ctgctgctca gggagcggct cgggttgctg acgcccgagc tgcgctaccg tctggcgggt
     4621 ggcagtctcc ggcagcgcgt gctggctgcc ttcattctcc ttcatcgcgg ctttcgcgtg
     4681 gcgcttgccg agagcgggct ggacgccgaa gtcgcgctcc atcctgagcc gcgcgctcat
     4741 ctttaagccc gcgttgcggg ggctgttgtg ccggtttaca gtcacctctc ccggcatgtt
     4801 gaccgaagcg cgagaagcga atgagttccg attgcctgtt ctgcagaatc gcgtccggcg
     4861 agatacccgg cgacatcgtc tacgaggacg atgagctgct cgccttcaac gacatcagcc
     4921 cgcaggcgcc gcatcacgtg ctcgtcatcc cgaagattca cgtcgccacc gtgaacgacc
     4981 ttgcggccga acacgccgat cttgtcggtc gtctggtgct ctgtgcccgg gccatcgccg
     5041 ccgagcgcaa cattcaggag tcgggctacc gcctgatcct caactgcaac gggcacggcg
     5101 gccagacggt cttccacctt cacctccacc tgctgggcgg ccggcagctg atggcgctgg
     5161 gctgacgtgg ctggtcctgg ctctttggct gatgccctgg tggcgcaggt agacaaagcg
     5221 ctgcgcacgc tgacgggcgc tgcagcttcg gtccgtgaga atcccgcggc cgatgtgcct
     5281 gaaggagaga tgagccctgg cgagcgggag cacgctgccg gcctgatgag ggtgaatcac
     5341 accggcgaga tatgcgccca ggcgctctac gagggccagg cgctgacggc gtcggacccc
     5401 cgcacccgga cagcgctgct cgcggcagcc tccgaagagt cggaccacct cgcctggtgt
     5461 gccggccggc tcgccgagct gggcagcagc ccgagcgtgc tcaacccggc cttctacgcg
     5521 ctttcctatg ccctgggggc ggtgacgggc gttctcggtg acaaggtcag tctgggattc
     5581 gtggaggcga cggaggatca ggtctgccga cacctcgagt cgcacctgca gtccctgccg
     5641 gccggcgatt cccgcagccg tcagatcgtt cgcgccatgc gggaagacga agcgcgccac
     5701 ggggccgagg ccctgggagc cggcggggcc gagtttcccc ggccggtgaa acgggcgatg
     5761 tcgctggcgt ccaggctgat gaccgagacg acctaccgcg tctgaccacc cggtcgggaa
     5821 atcggcggat gatagatgag ctgcaccacg ttgccgtcgg ggtcctcgca gtagaacgat
     5881 cgcgcaccgt cgcgatgggt cctgggtctc tcgaggatgt tcacgccgtt ggcggccagg
     5941 tggtcgtgcc aggcgtccac atcttcctcg tcgtcgatga tgaagccgat gtggtccagg
     6001 gtctggccgc gcacggagcg gccctcggtt gcccggtgca gcgccaggtt gtccgttccg
     6061 gacgtgaggt agaagttgtc gggatccgga ttccactcca cctgcatgcc gaggagctcg
     6121 gtgtagaagc gcacggaggc ttccagcgtg gtggtgaaca gcgccacgtg ccgcagccct
     6181 gcatgcggct tgggtggttt ggtcatctgc ggaggttccg gagcttcatg cttccagtat
     6241 ttcgtagtcg tggctgattg caacaccgcc gcgctcgagc atgatcgaag cggagcagta
     6301 cttctccgcc gacagcgcca cggctcttgc gaccttggcc tcgtccaggc cctgcccgct
     6361 gatggtgaag tgcagatgaa tggcctcgaa cacctggggt accgtgtcgg cgcgccgggc
     6421 tgtgagcctg gtcacacagc cgacgacggg ctgccgcgac ttcctgagaa tgctgaccac
     6481 gtcgaacgac gcgcagccgc ccagacccat gagcacgagc tccatcggcc gggtacctct
     6541 gttctcgccg cccgcgtccg gcggaccgtc gatgagcacc gtgtgcccgg agtctgccac
     6601 ggcttcgaaa tgaacgttct cgatcagcgt gatttttgct tgcatggccg gcagtttccg
     6661 atatctggat gcccgatacg tgaggctggg agcgtctgag tagtgatggg ggaccactca
     6721 tactgtgaaa tggatacgtt tgtcgaccag ttctcgttgt ggttgtaaac gatatgcaag
     6781 tattcatccg caaggttcac aaggagcgga aatgcttgcc aacaagaatt cacagttctc
     6841 agggatggga aaaccactga ccgccaaggg gttcaacccg aacgccgggc aggaagatac
     6901 gcatctggat gcctttctgg cagcggccca tcgccgcaag tatccggcca agagcacgct
     6961 catctacgcc ggtgacaaga gcgattcgat ttactacatc ctcaagggct cggtaaccgt
     7021 gctcgtggag gacgaatccg gacgtgaaat catcgtcgcc tatctcaaca agggcgattt
     7081 cttcggcgag atgccgcttt tcacggaaag cgcgccgcgt tcggcctgga tcaaagccaa
     7141 gaccgagtgc gaagttgccg aactcagcta cggcaagttc agggaactcg cccagcgcca
     7201 cagcgatctg ctctacgccg tgggccggca gatggccgat cggctcggcc gcactaccca
     7261 gaaggtcagc gatctggcgt tcctggacgt gaccggccgc gtcgcgcgta cgctgatcga
     7321 gctcaccaag cagcccgatg ccatgaccca tcccgatggc atgcagatca agatcacccg
     7381 ccaggagatc ggccgaatcg tcggatgctc ccgcgagatg gtggggcgtg ttctgaagac
     7441 gctggtcgag cagggtctgg tgaacgtcaa aggcaagacg atggtggttt acggcacccg
     7501 ctaagacccc agcaggcgcg ccgaaaggaa acggcgcgcc cggttgctca cacaggacgt
     7561 ggaacagcgg gctctctgcc agatccggcg gagagcccgt tttccgtttc tgctgcaggg
     7621 acgcggccga gggtcgagcc cggcggtcct aagcgaacat ctcggcgagc gccgtccccg
     7681 gatccggcgc gcgcatgaac gcttcgccga cgaggaagca gtgcacgccg tgctgccgca
     7741 tggccgcgac atcctcgcgg tttgcgatgc cgctctccgt caccaccgtc acgctgtcgg
     7801 gaatgcccgg caggagtccg taggtggttt caagccgcgt ctcgaaggtc ttcaggttgc
     7861 ggttgttgat gccgaccagg cgaggctcga tctccagggc cgactcgagc tcctccccgt
     7921 cgtggacctc cacgagcacg tccagaccga gcgcgatggc cgtttcgtgc agggccttga
     7981 gctgtcccgc gtcgagggcg gccgctatga gaagcacgca gtccgcgccc attgcgcgtg
     8041 cttcgaacag ctgatatgcg tccaccacga aatccttgcg cagcacgggc aggtccacgg
     8101 ccgctcgggc ggcctgcagg tactcgtcgt ggccctggaa gtagcgctcg tcggtaagaa
     8161 cggagaggca ggcggcgccc cccttggcgt agctccgggc aatggacacg gggtcgaaat
     8221 cttcccgtat gacgcctttg ctgggcgatg ctctcttgat ctctgcgatc acgcctgaag
     8281 cgccggccaa aagcttgctt tccagcgcat ctgcaaagcc gcgggcgggc gggcacgcct
     8341 gggccagggc gcgcagggaa tccaggcttc gcacccgctg gcgctcggcc acctcggtgc
     8401 gtttgtgggc gagaatctcg tcgagaatgg tcacggcgcc gccaccatca cggctcttct
     8461 accgtcacgg ctcttccacc ttcacggctc cgccatcatg gaagagaccc gaacgagctc
     8521 ggcaaggcgt tcgctggcga gccctgcggc gatggcgtcc tgggccatga ccacgccgtt
     8581 cttgaagctc gtggcgacgc cggccgcgta gatcgccgcc cccgcattga ggctgacgat
     8641 gtcggcggcc gatgagtcgg gctcggtcag ggagcgtttc agcagggcaa ggctggtctc
     8701 cggagaatcc gctcgcagct cgtcgtggga ggaaggcttc aggccgaagt cggcgggctc
     8761 aatctcgtac tcggtgatgg tgccggcgtt gagctccacc acgaagctcg gcccgttcag
     8821 ggcgagctcg tccaggccgt tgctgtgcac caccagcaca tggctcgcgc cgagcagctt
     8881 gaggacctcg gccagggtgg tctgccaggc cgcgctgaag acgcccagca cctggttgcg
     8941 cgcgccggcc gggttggtca gcggccccag gacgttcatg atcgtccgtg ctccaagctc
     9001 ctggcgaacg ggcgcggcgt gacgcatggc gctgtgatga gcctgcgcga acaggaagcc
     9061 caccccaacc tcgcggatgc agtgcgcgat ctgctccggc gcgagggaga tgttcacgcc
     9121 ggcggcctcg agaacgtcgg cgctgccgct gaagctcgtc atcttgcgat tgccatgctt
     9181 ggcgacgtgg gcgccggcgg cggcggcaac gaacgtggcg gcggtcgaga cgttgaacag
     9241 cttggcgccg ctccccccgg taccgcaggt gtccacgagg tagggcacgt ccacgacgac
     9301 cttggtggac agggaccgca tggcctcggc ggccccggcg atctcttgcg gcgtctctcc
     9361 cttcacgcgc agcgcggcga gcagcgcgcc gatctgtccc ggggtggcgt ggccggacat
     9421 gacctcgacg aataccgccc tggcgtcctc cagggataga tcccgttttt cgaacagagc
     9481 ggccagcgca ccttgtatgt ccatgtcctt cacccctcca gaaagtttct cagcaggtcg
     9541 tgacccaccg gcgtgcggat ggattcagga tgaaactgca ccccctcgat ggggtactcg
     9601 cgatgtttca atcccatgat ctcgtcgagc tcgccgtcgc gctccgtcca ggccgtgacc
     9661 tccaggcaat cgggcaggtc ctcgtgcgcg acgacgagag agtggtaacg ggtcgcctcg
     9721 aacggccggg gaaggccgtg gaacacgccc cggtcatcgt ggtggatgag cgacgtcttg
     9781 ccgtgcatga cctccctggc gcgccgcacc gtgccgccca tggcctggcc gatgctctga
     9841 tgccccaggc agatgcccag gatgggaatc gtcgggccga accgggtgac gacctccatg
     9901 gaaacgccgg cttcgtcggg cgtgcacggc cccggtgaga tgacgatctt ctccggggcg
     9961 agctcctcga tctcggtcag ggtgatctcg tcgttgcggt acacggccac ctcggctccc
    10021 agctcgctga gatactgcac cacgttgtag gtgaacgagt cgtagttgtc gatcatcagg
    10081 agcataagag tatccggtgg gccgggacgt tcgggttcgc tgccctcagg cttgccgtgc
    10141 ccgctgcgct gcggctgctc gttgactgga agccggctag tataaactcg acgcccagac
    10201 ggtcacaccg gttgcgattg aggaaggcaa gcatggaact g
//
LOCUS       DBNHOX010000544         5043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1393848, whole genome shotgun sequence.
ACCESSION   DBNHOX010000544 DBNHOX010000000
VERSION     DBNHOX010000544.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1393848"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /gene="gpmI"
                     /locus_tag="AC16UZ_12005"
     CDS             <1..1197
                     /gene="gpmI"
                     /locus_tag="AC16UZ_12005"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR6247452.1"
                     /translation="IDEHLVAAMELARRHGVRRVAIHGFLDGRDSPPTSAAGFMAAVV
                     GAAAAHSNGETQCFVATATGRYHAMDRDRRWDRIERAYNLLVRGEAPFHAANGRAALA
                     AAYERDESDEFVQPTAVHAEGELPVRMLDGDAVVFMNFRADRARQITRAILEPGFDGF
                     QRRSTVALSDFVMLTRYADDIDTSCAFEPAVLSNTLGEYLANLGKTQLRIAETEKYAH
                     VTFFFSGGREDPFAGEERLLIPSPKVATYDLAPEMSARKVTDHLVEAIASKQHDLIIC
                     NFANGDMVGHTGVFDAAVKAVETLDECLGRIETAIVEAGGQMLITADHGNVEQMRDHG
                     TGQAHTAHTCEPVPLVYIGPQSVKLNSGGTLSDVAPTLLELMHLEKPAEMTGHSVAQL
                     EERRSA"
     gene            1194..2378
                     /locus_tag="AC16UZ_12010"
     CDS             1194..2378
                     /locus_tag="AC16UZ_12010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR6247453.1"
                     /translation="MNSLPTTDWLPRSRAGRRAAGTRRLAIVVLSLLAPLSFSSEKED
                     AQRELEDVVGKLNALDVWLDEAEERRVRWLGEVKIKDREVASVSEAVDEATAALAVVI
                     RSLGKLEAEQRELQEKRERQATKIGEHLAAAYRLDGRDFVQLLLNQESPDSFDRMIRY
                     HRYFSSARLSALQEYQETLEALAANRLALEDRAAEQEARKAELAERQARLIEERDQRR
                     LLIAQLDAEAEDKAVQRQRLDTDRDRLERLLDELNRRAMELDGSAFVARKGSLPWPLK
                     GPLRSSFGRPRADGRMVWQGMLVAADEGSPVKAVFRGRLIFADWLRGFGLLAIINHGD
                     GYMTLYGHADSLTKTVGDWVEAGEVIARAGRSGGQQASGLYFEVREEGVPRDPLGWLA
                     KR"
     gene            2407..3585
                     /locus_tag="AC16UZ_12015"
     CDS             2407..3585
                     /locus_tag="AC16UZ_12015"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR6247454.1"
                     /translation="MRPITLVGIAISMVLGIGVGIGAYRAWLEPDVNGRHGQAFDEVL
                     RQVHEGYVDEVAKEKLLENALKGMLHGLDEHSDYLDHLSYEDLQAETSGQFGGIGIEL
                     NLVDGYFTVIRPITDTPASAAGLQPGDRLTEIDRESLKGKKMVDIISSLRGTPGTPVI
                     LGIRRDGGPKMEVSLIRAVIQDPSVDGRLLEPGYGYVRISQFQVSTGSRFTSAIRELA
                     HQNGESLRGLVLDLRDNPGGVLQSSVDVADALLEEGLIVYTEGRLPSSYLKYRATSGD
                     ILDGAPVVILINGGSASAAEVVAGALQDHGRAVVLGAKSYGKGSVQSVMPLSEDEAIK
                     LTTAYYYTPQGRSIEDTGIVPDVAVDEASGDDDDSGLLLGQALDLLKAEQGGGLHARL
                     "
     gene            complement(3595..4338)
                     /gene="hisA"
                     /locus_tag="AC16UZ_12020"
     CDS             complement(3595..4338)
                     /gene="hisA"
                     /locus_tag="AC16UZ_12020"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR6247455.1"
                     /translation="MLLIPAIDMKDGQCVRLRQGRMDDATVFSDDPLAMAAHWAEQGC
                     RRLHLVDLDGAFAGEPRNGALVAAIARELGEIPVQIGGGIRSLDIIERYLDAGVSQVI
                     LGTRAVQDPDFLASAAERHPGRIILGLDARNGMLATDGWDKTTQTSAVGFAMGASRLD
                     LAAIVYTDIERDGMLEGLNLAATVARAEAVETPVVASGGISCLDDLAALKAAFAGSSG
                     RLMGAITGRAIYAGTLDFRAGQALLDGSG"
     gene            complement(4339..4950)
                     /gene="hisB"
                     /locus_tag="AC16UZ_12025"
     CDS             complement(4339..4950)
                     /gene="hisB"
                     /locus_tag="AC16UZ_12025"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR6247456.1"
                     /translation="MATKTKPSGRSVTVSRETQETRIAVSVDLDGSGASRLTTGLAFF
                     EHMLTQIARHGLIDLDIRADGDLEVDAHHTVEDVGIVLGQAIGQAVGDKAGISRYGHA
                     YVPLDEALSRAVVDFSGRPGLTYEVDFVRPRIGDFDVDLLREFFQGFVNHAQVTVHLD
                     NLRGENAHHQAETLFKAFGRALRMAVAPDPRMAGQVPSTKGSL"
BASE COUNT          910 a         1678 c         1682 g          773 t
ORIGIN      
        1 atcgacgaac acttggtggc cgccatggag ttggctcgcc gtcacggcgt ccgccgcgtg
       61 gcgattcacg gcttcctcga cggacgggac agtcccccga cctcggcggc gggcttcatg
      121 gccgccgtcg tcggagccgc cgcggcgcac tccaacgggg agacccagtg cttcgtcgcc
      181 accgccacgg gccgctacca cgccatggac cgagaccggc gctgggaccg catcgagcgc
      241 gcgtacaacc tgctggtccg gggagaggct ccctttcatg cggcaaacgg gcgcgcagcg
      301 ctggcggccg cgtacgagcg ggacgaaagc gacgagttcg tgcagcccac ggccgtgcac
      361 gcggagggcg agctgccggt ccggatgctc gacggagacg ccgtggtctt catgaacttc
      421 cgcgccgacc gagcccgcca gatcacccgg gcaatcctgg agccgggctt cgacggtttc
      481 cagcgccgct ccacggtagc gctgtccgac ttcgtcatgc tcacccggta cgccgacgac
      541 atcgacacct cgtgcgcgtt cgagccggcc gtgctgtcca acacgctcgg cgagtacctg
      601 gcgaacctgg gcaagaccca gctgcgcatc gccgagaccg agaagtacgc tcacgtcact
      661 ttcttcttct ccggcgggag agaggatccc ttcgccggcg aggaacgcct gctcatccct
      721 tcaccgaaag tggccacgta cgacctggcg cccgagatga gcgcgcgcaa ggtcaccgac
      781 cacctggtgg aagcgatagc gtcgaagcag cacgacctga tcatctgcaa ctttgccaac
      841 ggcgacatgg tcggtcacac cggcgtgttc gacgcggccg tgaaggccgt ggagacactg
      901 gacgagtgcc tgggccggat cgaaaccgcc atcgtagagg caggcgggca gatgctcatc
      961 acagccgacc acggcaacgt ggagcagatg cgcgaccatg gcaccggcca ggctcacacc
     1021 gcccacacct gtgagcccgt gcccctcgtc tacatcggac cgcaatcggt caagctgaac
     1081 tccggaggaa ccctctccga cgtagcaccc accctcctcg agctcatgca cctggaaaaa
     1141 ccggcagaaa tgaccgggca ctccgtcgct cagctagagg aacgccgctc cgcttgaaca
     1201 gtctcccgac gaccgactgg ctaccgcgga gccgcgccgg gcgccgggcg gccggcacgc
     1261 gtcgccttgc gatcgtggtg ctttctctcc tggcgccgtt gagcttctcc agcgagaagg
     1321 aagacgccca gagagagctg gaggacgtcg tgggcaagct caacgccctg gacgtatggc
     1381 ttgatgaagc cgaggagcgc cgcgtgcgct ggctcggaga ggtcaagatc aaggaccggg
     1441 aggtggcctc ggtgagcgag gccgtcgacg aggcgaccgc cgcgttggcc gtggtgattc
     1501 ggtccctggg caagctcgag gcggagcagc gcgagctgca ggagaagcgg gagcggcagg
     1561 caaccaagat cggcgagcac ctcgccgccg cgtaccgttt ggacggtcgc gacttcgtgc
     1621 agctcttgct caatcaggaa tccccggact cgttcgatcg gatgatccgt taccaccgct
     1681 acttcagcag cgcccgcctg tctgccctgc aggagtacca ggaaactctc gaggcgctgg
     1741 ccgccaacag gctggccctg gaagatcgtg cggccgagca ggaagcccgc aaggcggagc
     1801 ttgcggagcg ccaggccagg ctaatcgagg agcgcgatca gcgcaggctt ctgatcgcgc
     1861 agctggatgc cgaggcagag gacaaagccg tccagcggca gcggctggac accgaccgcg
     1921 accggctcga acgtctgctg gacgagctca accgacgcgc catggagctg gacgggtccg
     1981 ccttcgttgc ccgcaaggga tcacttccct ggccgctcaa gggtcccctg cgctcatcgt
     2041 tcggacggcc acgggccgac gggagaatgg tctggcaagg gatgctggtg gccgcggacg
     2101 aaggctcgcc ggtaaaggcc gtttttcgcg gccggttgat tttcgccgac tggctgcgcg
     2161 gcttcggact gctcgccatc atcaaccacg gcgacggtta catgacgcta tacggccatg
     2221 ccgactcgct cacgaaaacc gtgggcgact gggtcgaggc gggggaggtc atcgcccgcg
     2281 ccggccgcag cggcggccag caggccagcg gcctgtactt cgaggtgcgc gaggaaggcg
     2341 tgccccgcga ccccctcggg tggttggcca aacgctgatg ctggtgccaa actccccggt
     2401 atgaacttgc gtcccataac tctggtcggc atcgcgatca gcatggtcct cgggatcggc
     2461 gtcggcatcg gcgcttaccg ggcctggctg gagcccgatg tgaatggccg gcacggccag
     2521 gccttcgacg aggtgctgcg ccaggtgcac gaaggctacg tggacgaggt ggccaaggaa
     2581 aagctcctcg aaaacgccct caagggaatg ctccacggtc tcgacgagca ttccgactat
     2641 ctggaccacc tcagctacga ggatctgcag gcggaaacca gcgggcagtt cggcggcatc
     2701 ggcatcgagc tcaacctggt cgacggctat ttcaccgtaa tccggccgat caccgacacg
     2761 cccgccagcg ccgcggggct ccagcccggc gacaggctga ccgagatcga cagagagtcg
     2821 ctcaagggca agaagatggt ggacatcatc tccagccttc gcgggacacc cggcacgccg
     2881 gtcatcctgg gcattcgccg cgacggcggc ccgaagatgg aggtcagcct gatccgggcc
     2941 gtaatacagg atcccagcgt ggacggccgg ctgctcgagc caggttacgg ttacgtccgg
     3001 atctcgcagt tccaggtgag caccgggagc cggttcacct cggccattcg tgagctggcg
     3061 caccagaacg gggaaagcct gcgcggcctg gtgctggacc tgcgggacaa cccgggtggg
     3121 gtcctgcaat cctccgtgga cgtcgcggat gccctgctcg aggagggcct catcgtgtac
     3181 accgagggtc gcctgccttc cagctatctg aaataccggg cgaccagcgg tgacatcctc
     3241 gatggcgccc cggtggtgat tctgatcaac ggcggctcgg catccgcagc cgaggtggtc
     3301 gcgggcgctc tccaggacca cggcagggcc gtcgtcctgg gagcgaaaag ttacggtaag
     3361 ggctcggtgc agtcggtaat gccgctgtcg gaagatgagg cgatcaagct gaccaccgcc
     3421 tactactaca cccctcaggg gcgatccatc gaggacaccg gcatcgtgcc cgacgttgcc
     3481 gtcgatgagg cttctggcga cgatgacgac tccgggctgc tgctcggcca ggccctcgac
     3541 ctgctcaaag ccgaacaggg cggagggctg cacgcgcgcc tgtagcgcgc gccttcaacc
     3601 cgagccgtcg agaagggctt gtccagcccg aaaatccagg gtgcccgcat agatggcgcg
     3661 acccgtgatg gctcccatca gccttcccga gctcccggca aaggcggctt tcagggctgc
     3721 cagatcgtcg aggcagctga tgccgcccga ggcgacgacg ggggtctcga cggcctcggc
     3781 acgggccacg gtcgccgcca ggttgagccc ctcgagcatg ccgtcccgct ctatgtccgt
     3841 atacacgatg gccgcaagat ccagtcgcga ggcgcccatc gcaaagccga cggcactggt
     3901 ctgggtcgtc ttgtcccagc cgtcggtggc gagcatgccg tttcgagcgt cgaggccaag
     3961 gatgatccgg ccagggtgcc gttcggctgc ggacgccagg aaatcggggt cctgaacggc
     4021 ccgggtcccc agaatgacct gggagacccc ggcgtccaga tatcgttcga tgatgtccag
     4081 gctgcgaatg ccgccgccga tctgcaccgg gatctcaccg agctcacggg cgatggcggc
     4141 caccagagcg ccgttgcgcg gctcgccggc aaaggcgccg tcgagatcca cgagatgcag
     4201 ccggcgacac ccctgctcgg cccagtgcgc ggccatggcc agcggatcgt ccgagaacac
     4261 cgtggcgtcg tccatccgac cctggcgaag cctcacgcac tggccatctt tcatgtcgat
     4321 ggccgggatc aggagcatct agaggctgcc tttggtggac ggcacctgcc cggccatgcg
     4381 cggatccggg gcgacggcca tgcgcagcgc ccggccgaac gccttgaaaa gcgtttcggc
     4441 ctgatggtgg gcgttctccc cgcgcagatt gtccagatgc actgtcacct gagcgtggtt
     4501 gacgaatccc tggaaaaact cgcgaaggag gtcgacgtcg aaatcgccga ttcggggccg
     4561 cacgaaatcc acctcgtagg tcaggcccgg gcggccggaa aagtccacca cggcacggga
     4621 gagcgcttca tccaggggaa cgtaagcatg cccgtagcgc gagatgccgg ccttgtcgcc
     4681 gacggcctga ccaatggcct ggcccaggac gatgccaacg tcctccaccg tgtggtgggc
     4741 atccacctcg aggtcgccgt cagcgcggat gtcgagatcg atgagcccat gacgggcaat
     4801 ctgcgtgagc atgtgctcga aaaaggcgag ccccgtggtc agcctggacg cgcccgagcc
     4861 gtccagatcc acgctaacgg cgatccgcgt ctcctgggtc tcccgggaga cggtaaccga
     4921 gcgaccgctg ggcttggttt tcgtggccat gatcaaccgc cttccgcttc agtgaggccg
     4981 cgcaagtata cggactcgaa cccgacgact acagccggtc catcaggctc ccgtagggca
     5041 gaa
//
LOCUS       DBNHOX010000545         6182 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_43466, whole genome shotgun sequence.
ACCESSION   DBNHOX010000545 DBNHOX010000000
VERSION     DBNHOX010000545.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_43466"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..561
                     /locus_tag="AC16UZ_12030"
     CDS             100..561
                     /locus_tag="AC16UZ_12030"
                     /inference="COORDINATES: protein motif:HMM:NF045246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit-like protein"
                     /protein_id="HIR6247457.1"
                     /translation="MEDAPLIRGIDADPVDICLRYASGESMDSIAADYICKPNILRLW
                     MTSNPVRAEQWYQAQIHRAFGLADEMLEVARGLQPRMVPDADGKPRRWVPGSDEIASA
                     RVLIDTIKWQTGKLAPEIYGDRIKQEIDVRVSHEEALRTIIEMETPQHGSE"
     gene            817..2343
                     /locus_tag="AC16UZ_12035"
     CDS             817..2343
                     /locus_tag="AC16UZ_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HIR6247458.1"
                     /translation="MDQNEVSFRHRCRKDFLYYAPRALKIRTKAGPIQPLVLNRAQLH
                     IHQQLEAQRAETGRVRAILLKGRQQGASTYVGGRYYWRVTHEIGYQAYILTHEADATA
                     NLFAMAQRYHEFAPPILRPKSGKDSSKELTFPGLGDGGYKVGTAGNKGAGRSSTIQLF
                     HGSEVAFWPHAAEHAKGVLQAVPDSPGTEVVLESTANGVGGYYHEQWQEAEVGASDFK
                     AIFVPWYWQTEYTRPVPPGFALTEDEVELVRLYGLSGEQLAFRRGKIAQLSANNQDGL
                     ASFRQEYPMSAPEAFQTSGGEMALIQPGPVLLARRPEAKIEPYGPLMIGVDPARFGDD
                     RTSVIFRRGRKAYGLVSYSKLDTMAIVGIVVTLIREHQPDRVFVDVVGLGAGVVDRLR
                     ELGYSDRIVAVNGAERPTNYERYLNKRAEMWGEMKAWLTDPAQRVEIPDSDTLQADLC
                     GPWHKFDSNGRMELEKKEDMKKRGIRSPDEGDALALTFATVGAHIESFTPEAEHLAEY
                     "
     gene            2401..3222
                     /locus_tag="AC16UZ_12040"
     CDS             2401..3222
                     /locus_tag="AC16UZ_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247459.1"
                     /translation="MAITYRSAKGSGLTNDEIDGNFSDLDGRVTANTAAVAAAQSDAT
                     SALAAAAASSQPGHTHAASDITTGQITLTSAINPGGSFVSSGNRPWKDLIGDINPKTT
                     GPGAPTLAAFRGGQIRWFHYSVSDDGDVVFHMPHDYAPGTDVFIHVHWGHNGTNISGT
                     FAANLIHSWAKGFAQAAHPAEKTLPISVGSLNLTNTPQYAVRVDEVQLSVSGGSATQI
                     DSDDLEPDGLILLHYDVTTIPTITGGSAEPFIFAIDIHYQSTEIGTLNKQPDFYA"
     gene            3219..3416
                     /locus_tag="AC16UZ_12045"
     CDS             3219..3416
                     /locus_tag="AC16UZ_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247460.1"
                     /translation="MSQTYAAEDYFAQDYAAGPGAVFDLTDRVRIRRSTMQAQVFLTI
                     GPPTPQVEYEFSGGRTFKEPS"
     gene            3413..3745
                     /locus_tag="AC16UZ_12050"
     CDS             3413..3745
                     /locus_tag="AC16UZ_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247461.1"
                     /translation="MIIADVAGQLAKAEVSLAKRIAEELHKAYPGYAWGVTVNRSVLN
                     VLNLSLSGRWGFTLHITKLASDVKTAAIKAGGELLERYRQPRGRANDELILSAARDFK
                     GDLHCDRA"
     gene            3732..5840
                     /locus_tag="AC16UZ_12055"
     CDS             3732..5840
                     /locus_tag="AC16UZ_12055"
                     /inference="COORDINATES: protein motif:HMM:NF049598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HIR6247462.1"
                     /translation="MIELDDEVREDQYEEPDRDEPNWLNMSENAFRESTDWIDANKRR
                     QWERNISNFHSRHASGSKYSSDAYRGRSRLFVPKTRATERSNEASAAAAYFSTGDVLN
                     IAAVDPKNPLQRVSANMHKELIDFRLKKTIPWFMTLMGAFQDAQVTGVCISHQWWEFL
                     ESEEGKILADKPRIDLIPVENFRFHPASSWVDPIGTSPYLIYQYPMTVGDVKSRMNGR
                     MNGPQWKALSDGEISHGVVTEEDSTRLTREKNATGKHDTSAVNDFTPVWIHMNIMRHE
                     GTDYVFWTIGTKHMLTDPVPLEEVFHHGMRPFTLGFTNIETHRTYPSAAVELGQDVQV
                     AINDTVNQRIDNVSLVLNKRSWVKRGQNVDMRALSRSVPGGIVLMDDIDRSVKHEPTQ
                     DVTGSSYQEQDRLQMMFDDVSGAFSSATVQGSRALGETVGGMELMSDGTNAIKEYQLR
                     IFNESWVEPTLRQLQALEALYETDEVVLKLAADAIELIDTLRNPQLMGIVLEQDLTLE
                     ISVGMGATNPQQRISKINQGLQILGPLLGPRINVDEISMEVWGALGYKDGKRFVKPAE
                     EVEPPQAPVDPVKMAMAQVQQGLLNLKVEQFQFESQLAVQKLQAEFENKRVEHALRQA
                     EIVGRRDVMIAQIASRENIALEQVRANFDSTSFDRELKQSELALKQAEIETNRQIAAI
                     RERSKMAELQFKATSGREGI"
     gene            5840..6154
                     /locus_tag="AC16UZ_12060"
     CDS             5840..6154
                     /locus_tag="AC16UZ_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247463.1"
                     /translation="MNEIEEAMQTMSPSQREAIAEAVLGIEAQTFMASDVGRYIMGRA
                     QIDEEDATRRLIESDPSDVKEITKQQNRIWRARSLRQWLLEAIDGGVEAEYELRQQEN
                     EH"
BASE COUNT         1305 a         1943 c         1940 g          994 t
ORIGIN      
        1 ggcaccattg atggggtacg attttaggag acgtttcgag agggctctaa tatgtatagt
       61 gcgaaaatgt tggcccgggc ggctcgggag tacgagaaat tggaagacgc gccgctgatc
      121 cggggcatag acgccgaccc ggtcgatatc tgcctccggt acgcatcggg cgaatcgatg
      181 gattcgatcg cggcggacta catatgcaag ccgaatatcc tgcggctctg gatgacgagc
      241 aacccggttc gcgccgagca gtggtatcag gcccagatcc accgtgcgtt tgggctcgcc
      301 gacgagatgc tcgaagtggc ccggggcctg cagccgagaa tggtccctga cgccgacggc
      361 aagccgcgtc ggtgggtgcc ggggtcagac gagatcgcat cggcccgggt gctgattgac
      421 acgatcaaat ggcagaccgg caaactggct cccgagatct acggggaccg gatcaaacag
      481 gaaatcgacg tgagggtgtc gcacgaggag gcgctccgga cgattatcga gatggagacg
      541 ccccaacatg gctcagaatg acgaatacct aaagatcctg cccgaggggt acgggctggt
      601 ggctcattac aaactgggca atttccgacg agacgtgctc gtctgcctgc actgcggacg
      661 ccagaagatc gtctgccacc gctacatcca tcggccatgt ttttgccgac ggaatccccc
      721 gccgccctgc cagatctgcg gcgatccggc atcgagcaaa ctgtctaaat tctgtagcag
      781 ctgccgccgg attcggcgca tagagaggat tcggccatgg atcaaaacga agtaagtttc
      841 cggcatcgtt gccgcaagga tttcctctac tacgccccgc gggccctcaa gatccgcacg
      901 aaagctggcc cgatccaacc gcttgtgctc aaccgggcgc agctgcatat ccatcagcaa
      961 ctggaggcgc agcgggccga gacgggccgg gtccgggcga tcctgctcaa aggacggcag
     1021 cagggggcga gcacttacgt gggcggccga tattattggc gcgtgactca cgagatcggc
     1081 tatcaagcgt acatcctgac gcacgaagcc gacgcgacgg ccaatctttt cgctatggct
     1141 cagcgctatc acgagttcgc gccgcccatc ttgcggccca agtcaggcaa ggattcgagc
     1201 aaagaactga cgtttcccgg cctcggcgac gggggctaca aagttggcac ggctggtaac
     1261 aaaggcgcgg gccgatcgag cacgattcag cttttccacg gctccgaggt ggctttctgg
     1321 ccccacgccg cggaacatgc caaaggcgta ttgcaggcgg tcccggacag cccgggaact
     1381 gaggtggtgc ttgaatctac ggcaaacgga gtcggcggct actatcacga gcaatggcaa
     1441 gaggctgagg tcggggcgtc cgatttcaag gcgattttcg tgccgtggta ttggcagact
     1501 gaatacaccc gccctgtgcc accgggcttc gcgctgacag aggacgaggt cgaactggtc
     1561 cgactctacg ggctgtccgg cgagcagctc gcattccgcc gcggcaaaat cgcccagctc
     1621 tcggccaaca atcaggacgg actggccagc ttcaggcaag agtacccaat gagcgccccc
     1681 gaggcgttcc aaacgtccgg cggcgagatg gccctgatcc aaccggggcc ggtgctgttg
     1741 gctcgccgac ccgaggccaa gatcgagccg tacggccctc tcatgattgg cgtggatccg
     1801 gcccggttcg gcgacgatcg cacctccgtt attttccgcc gcggtcgcaa ggcctacggg
     1861 ctcgtcagct acagcaagct cgacacaatg gcgatcgtcg gaatcgtggt cacgctgatc
     1921 cgcgagcacc agcctgaccg ggttttcgtc gatgtcgtgg gcctcggcgc gggcgtcgtg
     1981 gatcggctcc gcgagctggg ctacagcgac cggatcgtgg cagtcaatgg cgcggagcgg
     2041 cccaccaact acgagcgcta cctcaataag agggctgaga tgtggggcga gatgaaggcg
     2101 tggctcaccg acccggccca gcgggtcgag atccccgaca gcgacacgct gcaggccgac
     2161 ctgtgcgggc catggcacaa gttcgactcc aacggccgga tggagttgga gaaaaaagag
     2221 gatatgaaga agcgggggat ccggtcgccg gatgagggcg atgccttggc gctcacgttt
     2281 gcgacggtcg gagcgcacat cgagtcattc actcccgagg ccgagcacct cgccgaatat
     2341 taacgtcgcc ccttccgctc gcggcgtggt agtaagcggg gcaacttccg gaggcatcag
     2401 atggcaatca cgtaccgctc ggccaaaggc tcgggcctga caaacgacga gatcgatggc
     2461 aatttcagcg acctcgatgg ccgggtcact gctaacacgg cagccgtagc ggccgcccag
     2521 agtgacgcga cgtccgccct cgcagcagcc gcggcttcga gccagccggg tcacacccat
     2581 gcggccagcg acatcacgac cggccagatc accctgacct ccgcgatcaa cccgggcggg
     2641 tccttcgtct cgtcgggcaa taggccatgg aaggatttga tcggcgacat caatcccaag
     2701 acgaccggac cgggcgctcc gacgctggcg gccttccggg gcggccagat ccgatggttc
     2761 cactattcgg tcagcgacga tggggacgtg gtatttcata tgccacacga ctatgccccc
     2821 ggcaccgacg tattcattca cgtgcattgg ggccacaacg gcacgaacat ctccggcacg
     2881 ttcgccgcca atctgattca ctcgtgggcc aagggcttcg ctcaggcagc ccaccccgca
     2941 gagaagaccc tgccgatctc cgtcggctcg ctcaacctga ccaacacccc gcaatacgcg
     3001 gtgcgggtcg atgaggttca gctctcggtc tccggcggca gtgcgaccca gatcgactct
     3061 gacgacctcg aacccgatgg cctgatcctg ctgcactacg acgtcacgac catcccgacc
     3121 atcacgggcg ggagcgccga gccgttcatc ttcgcgatcg acattcacta ccaatcgacc
     3181 gagatcggca cgctcaataa gcagcccgac ttctacgcat gagccagacc tacgccgccg
     3241 aggactactt cgctcaggac tatgcagcgg gccccggcgc ggtgttcgac ctcaccgacc
     3301 gggtccggat ccgccgctcg acgatgcagg cacaggtctt tctcaccatc ggtccgccga
     3361 cgcctcaagt ggagtacgag ttcagcggcg ggcgaacatt caaggagcca tcatgatcat
     3421 cgccgacgtg gcggggcagc tcgccaaggc cgaggtcagc ctcgcaaagc ggatcgccga
     3481 agagttgcac aaggcctacc ccggctatgc atggggcgtc acggtcaatc ggagcgtgct
     3541 gaacgtcctc aacctgtccc tgtcgggccg gtggggcttc actctccaca tcaccaagct
     3601 cgcctcggac gtcaagaccg cggcgatcaa ggcgggcggc gagctgttgg agcgataccg
     3661 gcagccgcga ggccgcgcca atgatgagct gatcctgtcg gcggctcgtg atttcaaggg
     3721 agacctgcac tgtgatcgag cttgacgacg aggtccgcga ggaccagtac gaggagccgg
     3781 accgagacga gccgaactgg ctcaacatgt ccgagaacgc cttccgcgag agtaccgatt
     3841 ggatcgacgc caacaagcgg cggcagtggg agcggaacat ctccaatttc cactcgcgcc
     3901 acgccagcgg ctcgaaatac tcgtccgacg cctaccgggg ccggtcgagg ctattcgtac
     3961 ccaagacccg ggcgaccgag cgaagcaacg aagccagcgc agctgcggcc tacttctcga
     4021 cgggcgacgt gctcaatatc gcggcggtcg atcccaagaa tccgctgcag cgggtcagcg
     4081 ccaacatgca caaggagctg atcgacttcc gcctcaagaa gacgatccct tggttcatga
     4141 cgctgatggg ggcgtttcag gacgctcagg tcaccggggt ctgcatctcg caccaatggt
     4201 gggagttcct cgaaagcgaa gagggcaaga tcctcgccga caagccgcgg atcgatctga
     4261 tcccggtcga gaatttccgg ttccacccgg cgtcgagctg ggtcgatccg atcggcacct
     4321 cgccctacct gatctaccag taccccatga cggtcggcga cgtgaagagc cgcatgaacg
     4381 ggcgcatgaa cgggccacaa tggaaggccc tgtcggacgg tgagatcagc cacggcgtcg
     4441 tcaccgagga agactcgacc cggctgaccc gcgagaagaa cgccaccggc aagcacgaca
     4501 cgtcggcggt caatgacttc acgccggtct ggatccacat gaacatcatg cggcacgaag
     4561 ggaccgacta cgtcttctgg accatcggca cgaagcacat gctcaccgac ccggtccccc
     4621 tcgaagaggt cttccaccac gggatgcgcc ccttcacgct gggcttcacc aacatcgaga
     4681 cgcaccggac ctatccgagc gcagccgtcg agctgggtca ggacgtgcag gtggcgatca
     4741 acgataccgt caatcagcgg atcgacaacg tctcgctggt gctcaacaaa cgcagctggg
     4801 tcaagcgcgg ccagaacgtc gatatgcggg ccctgtcacg atcggtcccc ggcggcatcg
     4861 tcttgatgga cgacatcgat cgtagcgtca agcacgagcc gactcaggac gtcactggca
     4921 gctcgtatca agagcaggac cggcttcaga tgatgttcga cgacgtctcg ggcgcgttca
     4981 gctcggcgac ggtgcaaggg tcccgggctc tgggcgagac cgtcggcggc atggaactca
     5041 tgagcgacgg cacgaacgcc atcaaggaat accagctgcg catcttcaac gagagctggg
     5101 tcgagccgac gctgcgccag ctacaggctc tggaggccct ctacgaaacg gacgaggtcg
     5161 tcctcaaact ggccgccgac gccatcgagc tgatcgacac gctgcgcaac ccgcagctca
     5221 tgggcatcgt ccttgaacag gacctgaccc ttgagatctc ggtcggcatg ggcgcgacca
     5281 atccgcagca gcgcatctcg aagatcaacc aaggcctgca gatcctcgga ccgctgctcg
     5341 ggccgcggat caacgtcgat gagatctcga tggaggtatg gggcgctctg ggctacaagg
     5401 acggcaagcg gttcgtcaag cctgccgaag aggtcgagcc gcctcaggca ccggtcgatc
     5461 cggtcaagat ggccatggct caggttcagc agggcctcct caacctcaag gtcgagcagt
     5521 tccagttcga gtcgcagctg gcggtccaga agctacaggc cgaattcgag aacaagcggg
     5581 tcgagcacgc cctcaggcag gccgagatcg tgggccgccg agacgtcatg attgcccaga
     5641 tcgccagccg cgagaacatc gcactggaac aggtccgggc gaacttcgat tcgaccagct
     5701 tcgatcgtga gctgaagcag tccgagctgg cgttgaagca ggccgagatc gaaacgaacc
     5761 gccagatcgc cgcgatccgc gagcgctcca agatggcgga gctgcagttc aaagcgacat
     5821 ccggacgtga gggcatctga tgaacgagat cgaggaagcg atgcagacca tgagcccgtc
     5881 ccagcgggag gcgatcgccg aggcggtcct tgggatcgag gctcagacct tcatggccag
     5941 cgacgtcggc cgctacatca tgggccgcgc ccagatcgac gaagaggacg ccacccggcg
     6001 gctgatcgag tcggacccgt ccgacgtcaa ggaaatcacc aagcaacaga accgaatctg
     6061 gcgggcccgc agcttgcgcc agtggttgtt ggaggctatc gacggaggcg tcgaggctga
     6121 atacgagctg cgacaacagg aaaacgagca ctgacatgac gacagacacc atccagaagg
     6181 ac
//
LOCUS       DBNHOX010000546         1959 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1264639, whole genome shotgun sequence.
ACCESSION   DBNHOX010000546 DBNHOX010000000
VERSION     DBNHOX010000546.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1264639"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..586
                     /locus_tag="AC16UZ_12065"
     CDS             155..586
                     /locus_tag="AC16UZ_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR6247464.1"
                     /translation="MERDRFPVVVHVLMFRGDELFLLRRASTGFMDGYYALPGGHQRA
                     GESVAQAARRECEEETGVTDLDLAPICVMPYRAGRHQGLNFVFEAASWLGEPRVAEPE
                     LFSEGVWAAARDLPEPHAMWISDALQCRDEGDWFREFLWHG"
     gene            701..1675
                     /locus_tag="AC16UZ_12070"
     CDS             701..1675
                     /locus_tag="AC16UZ_12070"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR6247465.1"
                     /translation="MKDPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLEIT
                     PALNALQGVVMELEDCAFPLLHGVVATDDPNVAFKDATHALLVGSRPRSKGMERKDLI
                     EANAAIFSVQGKALNDHADRAVKVLVVGNPANTNCLIAQRNAPDLDPRQFTAMTRLDH
                     NRAAAQLAARTGKRVTDVAGLCIWGNHSATQYPDLHAATVAGTPAMELVDMAWYTGEF
                     IPTVQQRGAAIIEARGASSAASAANAAIDHMHDWSLGSEGVVSMGVYADGSYGVEPGL
                     IYSFPVICEGGDWQIVQGQEIGEFSREKMAATEKELVEERDAVAHMLS"
     gene            complement(1720..>1959)
                     /locus_tag="AC16UZ_12075"
     CDS             complement(1720..>1959)
                     /locus_tag="AC16UZ_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247466.1"
                     /translation="TAQPTSHWIFTLRDQLVSRSSRLLLRVGVWVLRQRMSSAEVGEY
                     LRETADRVASGHHEPGDPLESHARRCNWNHFFVAK"
BASE COUNT          338 a          571 c          695 g          355 t
ORIGIN      
        1 agttgctcca tggccggcca cgcgttcagt gacgcctcct cgatccgtcg ggtcaacgcc
       61 gcgtctggct cgcctgctcg ggtcatgggc ggggttgctc ggcatcattc acccgcgcac
      121 catagcagga tcaaaagggg tttgagcggg tctggtggaa cgcgatcggt ttccggtggt
      181 ggtgcacgtg ctgatgttca ggggcgacga gctctttctc ctgcggcgcg ccagcaccgg
      241 gttcatggac ggctactacg cccttccggg cggtcatcag cgcgcgggtg agagtgttgc
      301 gcaagccgct cggcgcgagt gcgaggaaga gacgggtgta accgacttgg acctcgcgcc
      361 gatctgcgtg atgccgtatc gagccggtcg gcatcagggc ctcaacttcg tcttcgaggc
      421 ggcgtcttgg ctgggcgagc cgcgcgttgc cgagccggag ctgttttcgg aaggcgtatg
      481 ggcggccgcg cgtgatcttc ccgagcccca cgccatgtgg atttccgacg cgctgcaatg
      541 tcgggacgag ggggattggt tcagggagtt tctctggcac gggtgacgtg cgaaagcgat
      601 ttgctttcct tacaatgcgc cgccgctgag ctatgggccg gcgtaacgac atgccgtatc
      661 ggcacgcgcg gccagtcgag attgtgaaag ggagtcagac atgaaagatc ccgtgcgggt
      721 cgcggttacc ggcgctgccg gtcagattgg atatgcattg ctgtttcgaa ttgcttccgg
      781 ggagatgctg ggaaaggatc agccggtgat cctgcagctc ctggagatca cgcctgcgct
      841 gaacgcgctt cagggggtgg tgatggagct ggaagactgt gcgtttccgc tgcttcatgg
      901 agtcgtcgcc acggacgatc ccaacgtggc tttcaaggac gcaacccatg cgctgctggt
      961 cggctcacgc ccgcgaagca aggggatgga acgcaaggac ctcatcgaag cgaacgcggc
     1021 gatattctcc gtgcagggca aggcgctcaa cgaccatgcg gatcgcgcgg tgaaggtgct
     1081 cgtggttggc aacccggcga acacgaactg tctcatcgct cagcgcaacg cgcccgacct
     1141 ggatccccgg cagttcaccg ccatgacccg gctggatcac aaccgtgcgg ccgcgcagct
     1201 ggcggcaagg accggaaagc gggtgacgga cgttgccggg ctgtgcatct ggggcaacca
     1261 ttcggccacg cagtatcccg atcttcacgc ggcgaccgtt gccggcacgc ccgccatgga
     1321 gctcgtcgac atggcgtggt acacggggga gttcattccc acggtgcagc agcggggggc
     1381 cgcgatcata gaggcccgcg gagcgtccag cgcggcttcg gcagcgaacg ccgccatcga
     1441 ccacatgcat gactggtctc tgggcagcga gggcgtggtg agcatgggcg tctacgcgga
     1501 tggcagctac ggcgtcgagc cggggctgat ctactccttc ccggtcatct gtgagggtgg
     1561 cgactggcag atcgtgcagg gccaggagat cggagaattc agccgtgaga agatggctgc
     1621 gacagagaaa gaactcgtcg aggaacgcga cgccgtagcg cacatgctga gctgaaacgg
     1681 cctgtagagg ctggcggagg cagcagcttt cgcaaaccgc tacttggcga cgaagaagtg
     1741 gttccagttg cagcgccggg cgtggctttc cagggggtcg ccgggctcgt ggtgaccgct
     1801 tgcgacgcgg tcggccgttt cgcgcaggta ctcgccgacc tcggcgctgg acatccgttg
     1861 tctgagcacc cacaccccga cgcgaagcag cagccggctg ctgcgcgata cgagctggtc
     1921 gcggagcgtg aagatccagt gcgacgtagg ttgagcggt
//
LOCUS       DBNHOX010000547         1918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1779178, whole genome shotgun sequence.
ACCESSION   DBNHOX010000547 DBNHOX010000000
VERSION     DBNHOX010000547.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1779178"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="AC16UZ_12080"
     CDS             <1..255
                     /locus_tag="AC16UZ_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247467.1"
                     /translation="KLLGTRLAGRILGWRRGEDALIGWLLQTKALIMIIFANVLLDRH
                     VITPTAFTALLLMAVGSTMLSIPMAQPRLARLLEEPAQRE"
     gene            complement(336..1058)
                     /locus_tag="AC16UZ_12085"
     CDS             complement(336..1058)
                     /locus_tag="AC16UZ_12085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR6247468.1"
                     /translation="MGPGRIAFNHLYIGVLVTVFYVIAGPILVSRGYPGLAPLLMAEL
                     FILAPVGMVHLLVKAKSLTGQYHLSSVVVYTNKIPLRSMILWTVGGLVLVNLVYVPLY
                     PVGIFLKNSVFAWLPKWYFDPGFGSTDAALIARMYLAAILIDGLVGPIVEELFFRGYL
                     LARMEHLKGWAPVVNGALFGIYHFWQPHNFLALVGVGIALSYVVWKTRNVYLAIIIHC
                     SMNLLGAVGGYMAASAGLMFSR"
     gene            1268..>1918
                     /locus_tag="AC16UZ_12090"
     CDS             1268..>1918
                     /locus_tag="AC16UZ_12090"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR6247469.1"
                     /translation="MHLLRQSAVLILSVSMAACGGRLVLPDTGPAGRALPACAGPASP
                     SVSHPLGAWGIALRTEADIPAAVAAMTIAEKVGQMVQADVSTASPDDVREYRLGSITD
                     TVPAGDRGTTAAWRELFDAYRKAALQTRTGIPIVVGVDAVHGHAYFDGNSVILPHNIG
                     LGATRDPELVAQLARVTARELSATGIRWTFSPTIAVARDIRWGRTYESFGEDVELQR"
BASE COUNT          376 a          622 c          606 g          314 t
ORIGIN      
        1 aagctgctgg gaacccgcct cgcgggacgc atcctggggt ggcggcgcgg cgaggacgcg
       61 ctgattggct ggctgctgca gaccaaggcg ctgatcatga tcatcttcgc caacgtgttg
      121 ctcgaccggc atgtcatcac gcccaccgcg ttcaccgccc tgctgctgat ggcggtcggc
      181 agcacgatgc tgagcattcc catggcacaa cccaggctgg cccggctgct ggaggagccg
      241 gcccagcgcg agtgagcagt cgtcgaggcg attcaaatcc cccgcttcag gcgcgtcgat
      301 tcgtcggcgt gcaaggcgcc gtcacattgc tgtggtcatc ggctgaacat cagtcctgcc
      361 gaggcggcca tatagcctcc gacggccccg agcagattca tcgagcagtg aatgatgatc
      421 gccagataca cgttcctggt tttccagacc acgtaactca acgcgatccc gactccgacc
      481 agggccagga aattgtgggg ctgccagaag tggtagatac cgaacaacgc gccattcacc
      541 accggagccc agcctttcag gtgttccatc cgggccagca gatagcccct gaagaacagc
      601 tcttcaacga tgggacccac cagtccgtcg atcaagatcg cggccagata catcctcgct
      661 atcagggccg catcggtact cccaaacccg ggatcgaagt accatttcgg cagccacgca
      721 aacaccgagt tcttgaggaa gatgccaacc ggatagagcg gtacatagac aaggttcacg
      781 agcacgagtc cgcccacggt ccacaggatc atggaccgca gcggtatctt gttcgtgtaa
      841 acgaccaccg acgacaaatg atactggccg gtcagggact tggccttgac cagtagatgg
      901 accatcccga caggcgccag aatgaacaac tcggccatga gcaagggggc aaggcccgga
      961 tatccccgcg agacgaggat ggggcctgca atcacgtaaa agactgtaac caggacgcca
     1021 atgtagaggt gattgaacgc gattcggccg gggcccattg gagtgagttg catgaccgtc
     1081 ctgagtcgcc aagagcgctg tccatgctaa acgctccttg cagcgtcgta aatcgtatgg
     1141 cagaaagcgc catcacgtcc ccgcggccgc tgcccacgct gcgggcgcgt cggcgtcaac
     1201 ccgttccggg caggctggca ggacagcccg tccgggaagc gccggatatg ctgctaacgg
     1261 atcccggatg cacctcttgc gacaaagcgc cgtcctgatc ctgtctgttt ccatggcagc
     1321 ctgtggcggc aggctcgtgc tgcccgatac cggccccgcc ggccgggcac tgccggcgtg
     1381 cgccggcccg gcgtcgccgt ctgtcagtca tccactggga gcctggggca tcgcgctgcg
     1441 gacggaggcc gacatacccg cggccgtcgc cgccatgacc atcgcggaga aagtggggca
     1501 gatggtgcag gcggacgtgt cgaccgcgtc cccggacgac gtccgcgagt accggctggg
     1561 ttccatcacc gacaccgtcc ctgcgggtga tcgtggaacg accgccgcgt ggcgggaact
     1621 cttcgatgcc taccggaagg cggcgctgca gacgcgcacc ggcattccga tcgtggtcgg
     1681 cgtcgatgcg gtgcacggcc acgcctactt cgacggcaac tcggtgatcc ttccgcacaa
     1741 catcgggctc ggcgcaacgc gcgacccgga actcgtcgcg cagctggccc gggtcacggc
     1801 cagggaattg tcggcgaccg gcattcgctg gacgttctcg ccgaccatcg cagtggcgag
     1861 ggacatccgc tggggcagga cctacgagtc cttcggcgag gacgtcgaac tgcagagg
//
LOCUS       DBNHOX010000548         3344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_878992, whole genome shotgun sequence.
ACCESSION   DBNHOX010000548 DBNHOX010000000
VERSION     DBNHOX010000548.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_878992"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="AC16UZ_12095"
     CDS             complement(<1..1220)
                     /locus_tag="AC16UZ_12095"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6247470.1"
                     /translation="MSEAVSNEAPFWLSGNFAPTFDEVTETRLEVTGTIPPELKGRYL
                     RNGANPESGVTPHWFLGNGMIHGVELNDGRANWYRNRYVRTPLLSDGDHSDGVDERAK
                     SLANTHIIGHAGRILALEEGHWPFELTPELDTVGPYTFGGRLETGMTAHPKICAETGE
                     LLFFAYAATPPFMTYHRATADGELVQSEVIEVKGATMVHDFNITRNYVIFMDLPVVWN
                     FENAASVGLPLAWSDEYGARLGVMPRNGSNADVVWYDIDPCYVYHPLNAYEDGDDIVI
                     DVCRMAHHMKIGAPEAPPMLHRWTIHQSAGTVSEAQLDERSVDFPRVPDALVGQKHRY
                     GYTAEFGGGLPAAVAFRKYDLATGTSEAHVLADGRAGGEPVFVPARGGTSEDDGYLMS
                     FVYDPADNRSELVI"
     gene            complement(1217..1867)
                     /locus_tag="AC16UZ_12100"
     CDS             complement(1217..1867)
                     /locus_tag="AC16UZ_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6247471.1"
                     /translation="MVAHSKSVTYTRAPAEKRERLLAAARELFAERGFEDTSTQQIAK
                     TAGVSEGILFHHFGSKRGLLERIAEDFVAAGVQAAMPDTPGQLSEEMVVRGAFDFADA
                     HPALYRLLEQMSRELGGSSGASRSAVIVAAIEKSLRAGMAQGIVREGDAGIMAELQFA
                     LVDAAYKAWRDGGDPDRREDYIREAVGCMKAMLAPSGAEERPPLADATKLSRGDAT"
     gene            complement(1934..3268)
                     /locus_tag="AC16UZ_12105"
     CDS             complement(1934..3268)
                     /locus_tag="AC16UZ_12105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR6247472.1"
                     /translation="MSNQRAAEFWARTRDVAGTWGLALALLLAGLIVAYQFVGPPPPK
                     RFVLATGESSGAYYYFGERFAQVMARDGVVVELRATGGSVDNLDLVAREGGADLAFVQ
                     GGIASSAPVDNLVTMGSLYLEPLWLFLGPGVVLDDLGDLKGRRLAVGVEGSGTRAVVM
                     QLLEANGVDAANSKLLGVASAALPDALAGGEIDAAFLIGAPQSSTVGKLLRLPGVRLH
                     DMERVEAYTRRNAFLSSVVLPRGVLDLEADLPAKDTETVAVGAMLVARDDFHPALTDL
                     LLIAAAEIHGGHSILADAGTFPTPRFVDLPLSEEAERHYRHGAPFLMRYLPFWAATLV
                     DRLWIMLVPLIGLSLPLARLVPPAYRWQIRRRLLRIYTKLHALDPLRTPIADDEDVAR
                     RLEELDRLDVDSTIEAVPKGYADDLYQLRRDIDLVRRRLGAARSAGRGQQND"
BASE COUNT          587 a         1140 c         1046 g          571 t
ORIGIN      
        1 atgatcacca gctcgctgcg gttgtccgcc gggtcgtaga cgaagctcat gagatatcca
       61 tcgtcttcgg acgtaccccc tcgtgccgga acgaagaccg gctcgccgcc cgcacgaccg
      121 tcggccagca cgtgcgcctc ggatgttccc gtggccaggt cgtacttacg gaaggccacc
      181 gcggcgggaa gtccaccacc gaactccgcc gtgtagccgt agcgatgctt ctgaccgacc
      241 agcgcatcgg gcacgcgcgg gaaatctacc gaccgctcgt ccagctgcgc ttcgctgacg
      301 gtgcccgccg actgatgaat ggtccagcga tgcagcatcg gcggcgcttc cggcgccccg
      361 atcttcatgt ggtgggccat gcgacagacg tcaatgacga tgtcgtcgcc gtcctcatag
      421 gcattcagcg ggtggtagac gtagcaggga tcgatgtcgt accacaccac gtcggcgttg
      481 gagccgttgc ggggcatcac gccgaggcgc gcgccgtact cgtcgctcca ggccagcgga
      541 agaccgacag aggcggcgtt ctcgaaattc cacaccacgg gcaggtccat gaagatcacg
      601 tagttccgcg tgatgttgaa gtcgtggacc atggtcgcgc ccttgacctc gatgacctcg
      661 ctctgaacga gctcgccatc ggcggtcgcg cggtgatagg tcatgaaggg gggcgtcgcc
      721 gcgtaagcaa agaagagcag ctcgccggtc tccgcgcaga tcttcggatg ggccgtcatg
      781 ccggtctcca gccggccgcc gaaagtataa ggaccaacgg tgtcgagctc cggggtcagc
      841 tcgaagggcc agtgtccttc ttccagcgcc agaatccggc ccgcgtggcc aatgatgtgg
      901 gtgttggcaa gggacttggc gcgctcgtcc accccatcgc tgtggtcccc gtcgctgagc
      961 aggggcgtgc ggacatagcg gttgcggtac cagttcgccc gaccgtcgtt cagctcgacg
     1021 ccgtgaatca tgccgttgcc cagaaaccag tgcggcgtga cgccggattc cgggttggcg
     1081 ccgttgcgca ggtaacgtcc cttgagctcc ggggggatgg tcccggtcac ctcgagcctg
     1141 gtctcggtca cctcgtcaaa ggtcggggcg aaattgcccg agagccaaaa gggtgcttcg
     1201 ttgctgactg cttcgctcat gttgcgtctc ctcgactgag tttcgttgcg tccgccagcg
     1261 gcggtcgttc ctcggctccg ctcggggcca gcatggcttt catgcagccg accgcttcgc
     1321 ggatgtaatc ttctctgcgg tccggatcac cgccgtcccg ccatgccttg taggccgcgt
     1381 ccaccagggc gaactgcagc tcggccatga ttccggcatc cccttcccgg acgatgccct
     1441 gagccattcc agcccgcagg ctcttctcga tggccgcgac gatgaccgcg ctgcggctgg
     1501 cgccactcga cccgccgagt tctcggctca tctgctcgag cagccggtac agtgccggat
     1561 gggcatcggc aaaatcgaac gccccccgca ccaccatctc ctcgctgagc tggccaggcg
     1621 tatcgggcat ggccgcctga acgccggcgg ccacgaaatc ctctgcgatg cgctcgagca
     1681 gccctcgttt cgaaccgaag tggtgaaaga ggatgccttc cgagaccccc gccgtcttgg
     1741 cgatctgctg agtcgaagtg tcttcgaacc cgcgctcggc aaacagctcc cgggcggctg
     1801 ccagcaatcg ctcccgcttt tccgcagggg ccctggtgta ggtaaccgat ttagagtgcg
     1861 cgaccattta ttgagtagca ctttacaaag tgttactcaa caagtaaacc cccaatttgc
     1921 ggcatgcagg ttttcagtcg ttctgctggc cgcggcccgc cgagcgagcc gccccaaggc
     1981 gccgccggac gaggtcgatg tcgcggcgaa gctggtagag gtcgtcggca tagcccttcg
     2041 gaaccgcctc gatagtcgag tccacgtcca gccgatcgag ctcctccagc cgacgggcca
     2101 cgtcctcatc gtcggcgatg ggcgttcgaa gcggatccag ggcgtgcagc ttcgtgtaga
     2161 tgcgcagcag ccgccgacgg atctgccatc gatacgcagg gggcaccagc cgcgccagcg
     2221 gcaggctcaa cccgatcagc gggacgagca tgatccagag ccggtcaacc agcgtggccg
     2281 cccagaacgg caggtagcgc atgagaaacg gcgccccgtg ccggtagtgc ctctccgcct
     2341 cctcgctgag cggcaggtcg acgaaacgcg gcgtgggaaa cgtgcccgca tccgcgagaa
     2401 tgctgtgacc accgtgtatc tcggcggccg cgatgagcag aaggtccgtg agcgccggat
     2461 ggaaatcatc acgggccacg agcatggccc ccacggcaac cgtctcggtg tccttcgccg
     2521 gcaggtccgc ttccagatcc agaacgcccc tgggcaggac gactgaagag aggaaggcgt
     2581 tgcgccgcgt gtaagcctcg acccgttcca tgtcgtgcag acgaactccc ggcaggcgga
     2641 gaagcttccc gaccgtggag gattgcggcg cgccgatgag aaacgccgcg tcgatctcgc
     2701 ccccggccag cgcgtcggga agcgcggctg acgccacccc cagaagcttc gagttcgccg
     2761 cgtccacccc gttggcctcg agcagctgca tcacgacggc gcgtgttccg ctgccttcca
     2821 cacccaccgc caaccgccgg cccttcagat cgcccaggtc gtcgagcacc acgcccggtc
     2881 caaggaacag ccagaggggt tccagataca ggctgcccat agtgacaagg ttgtccacgg
     2941 gggcagacga cgcgatgccc ccctgcacga aagcgaggtc ggccccccct tccctggcca
     3001 ccagatcgag attgtccacg ctgccaccgg tggcgcgcag ttcgaccacc accccgtccc
     3061 gggccataac ctgggcaaac cgctcaccga aatagtagta agcgccggac gactccccgg
     3121 ttgcgagaac gaaccgtttc ggcggcggcg gcccgacgaa ctgataggcg acgatgagcc
     3181 ccgccagcag gagcgcgagc gcgagccccc aggttcccgc tacgtccctg gtccgggccc
     3241 agaattcagc tgcacgttga ttggacatgc tcgaacggtc aacgcttctt cttcaggcgc
     3301 tcatggccgc gcgaatcctc gtcggccttc tgaaattcca cccc
//
LOCUS       DBNHOX010000549         5288 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1779245, whole genome shotgun sequence.
ACCESSION   DBNHOX010000549 DBNHOX010000000
VERSION     DBNHOX010000549.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1779245"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /locus_tag="AC16UZ_12110"
     CDS             <1..1385
                     /locus_tag="AC16UZ_12110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR6247473.1"
                     /translation="VDFPPLTPWLLALLGWIGGTDLIWLRGLACAAGIVTTLVAVDVC
                     RILGGRNFAQWTTAIVVLFAPAFMSVQSIFTMNVLDQLWWLLALSVLLRYLHDHQPRY
                     MILLGVIFGLALLTKLAIAAFILCLLGTSALYARWMFYRTESWAGSAIALSMLAPFVM
                     WQAVSGWPLLEFVQAYNVSEPEPMVLDQPLFGLIATMNPPFLLIWLPGLIYSLMAPKP
                     IRVVGSAAVATLVFFLAAGVKFYFATPLFALFTALGAVLWQRWLSSSGARSCLLAILL
                     ISGVAAVPIAAPVLAPTMLQQVANFIRDAEQDKVADDDAPIGRYFPHFAEMHGWPELT
                     EWVTRAYEGLPSARRPEVAVVAAYFGQAGALNQLDKADRLPVVHSGHMNYYLWWQADE
                     PNARLEDVLLVGFHPKELEPLFAEITILDRFSCNRCMTREQGAYLIRARRPLIDPEEI
                     RDRLKRFHFF"
     gene            1439..2395
                     /locus_tag="AC16UZ_12115"
     CDS             1439..2395
                     /locus_tag="AC16UZ_12115"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR6247474.1"
                     /translation="MSEARLGLEQAAPDSITKLDAIRHSVEARLRELSPSAAEGGERI
                     EHAIQYSLLDGGKRLRPLLTILTASTFDADPDLALDPACAIEMVHTASLVLDDLPCMD
                     DALQRRGKPATHVAHGEDIAILSAVSLISEAFGVIARAPGIAPEVRTSIVGRLADAIG
                     IQGLSAGQERDLHEAQSTTSRAALEKRHHQKTGALFVACLEAGARIAGVDETTMAAMT
                     KFGERVGIGFQILDDLLDLSASESTGKDAHRDEGKATFATVMTIQEAEREAITCLDNA
                     LAELADQIADAGPFAVFVDLVVRAYADQVSRRGSEIAGALRP"
     gene            2392..2877
                     /locus_tag="AC16UZ_12120"
     CDS             2392..2877
                     /locus_tag="AC16UZ_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6247475.1"
                     /translation="MTVVVNAACFLTALAIMEWVAWVTHKYVMHGFLWSLHESHHQPR
                     KGRFERNDWFAVFFAVPSIVLIYLGNNGYPAALWGGLGVAAYGLIYFIFHDVIVHRRL
                     DTGYAPRSRYMRRIVHAHRLHHAVSEKHGAVSFGFVYAPPIPVLRAEMKALAASGASG
                     S"
     gene            complement(2866..3795)
                     /locus_tag="AC16UZ_12125"
     CDS             complement(2866..3795)
                     /locus_tag="AC16UZ_12125"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014597913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HIR6247476.1"
                     /translation="MSDVFAASASIISRGSKSFAAAARLFDPTTRGHAIMLYAWCRHC
                     DDEIDGQDLGIGQRSPAPPEQRARLEQLRRDTEAAMRGEAVDGTPFVAFQRVFLECDI
                     PRNYPLELLDGFAMDVEGHECRTLSDTLRYCYHVAGTVGGMMATVMGIRDPEVVARAV
                     DLGIAFQLTNIARDVMQDAADGRCYLPRQWLADAGVPEDTGQFPDHRDVIYGQTIRIL
                     DEAERYYESARHGIGRLRFRNAWAIASALAIYRDIGRVVRRDGPDVWQHRATVGVGRK
                     LVLLLKGAALAGLQVTFGRRRTAPIRRGLWSAS"
     gene            complement(3796..>5288)
                     /locus_tag="AC16UZ_12130"
     CDS             complement(3796..>5288)
                     /locus_tag="AC16UZ_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007253234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HIR6247477.1"
                     /translation="LALAIRLQSAGFRTTLFEGRDRPGGRAYVYEDEGFTFDAGPTVI
                     TDPDCLRELFALSGRSLEDYLELTPIAPFYRLCWEDGYSFDYSNDPDETAAQIGAKNP
                     ADVDGYRRFLAYSEEVLKEGYVKLGSVPFLDFASMIKAAPQLVRLQSYRSVYSMVSRF
                     VKDEQLRQAFSFHTLLVGGNPFNTSSIYTLIHSLEQRWGVHFPKGGTGALVQGLVRLF
                     GDLGGELKLNTPVAEILTSGDRVTGVRTDNGWEGRFDAVASNADVVFTYERLLGRTAR
                     GRAAARALRRKRFSPSLFVVYFGVRGSYPELKHHSILFGPRYRELLKEIYAGPGLPDD
                     FSLYLHAPSVTDPSLAPPGCSTFYVLSPVPNLKAADLDWSELGPRYRDHILAYLEKHY
                     LPGLGERLVTTRTFTPDDFQTQLNAHLGSAFSLEPVLWQSAWFRTHNRDDVLKGLYFA
                     GAGTHPGAGIPGVVGSAKATSRLMLDDFGRTYPDSDRSDQFAKEMA"
BASE COUNT          928 a         1739 c         1643 g          978 t
ORIGIN      
        1 atgtggattt cccgccccta acgccatggc tgctcgccct gctcggctgg atcggcggca
       61 ccgatctcat ctggctgcga gggcttgcct gcgccgccgg catcgtcacc acgttggtgg
      121 cggtggacgt gtgccggatc ctcggcggcc gcaacttcgc ccaatggacc acggccatcg
      181 tcgtgctctt cgctccggcg tttatgtcgg tccagtcgat cttcacgatg aacgtcctcg
      241 accagctctg gtggctgctt gcattgtctg tgctgcttcg ctacctgcat gaccatcagc
      301 cccgctacat gatcctgcta ggcgtcatct tcggcctcgc acttctcacc aagcttgcca
      361 tcgccgcgtt catcttgtgc ctgctgggca ccagcgccct gtacgcacga tggatgttct
      421 accgaacgga gtcctgggcg gggtcggcca tagctctgag catgttggcc ccgttcgtga
      481 tgtggcaggc ggtttcgggg tggccgttgc tggagttcgt ccaggcttac aatgtcagcg
      541 aaccggagcc catggttctc gatcagcctc tcttcggcct catcgccacc atgaaccccc
      601 cgttcttgct gatctggctt ccgggtctga tttactcgct gatggctccg aagcccatac
      661 gggtggtcgg gagcgcagcg gtggcgaccc tggttttctt tctcgccgcc ggggtgaagt
      721 tctactttgc aacgccgctg ttcgcgctgt tcaccgcctt gggcgccgtg ctctggcagc
      781 gctggctttc cagctcgggc gcccgcagct gcctgctcgc cattctcctg atctccggag
      841 tcgcggccgt tccgattgcc gcccccgtgc tcgcgcccac catgcttcag caggtggcaa
      901 acttcattcg ggacgccgaa caggacaaag tcgcagacga tgacgctccc atcgggcgat
      961 actttccgca ctttgcggag atgcatggat ggcctgagct gaccgaatgg gtgacccggg
     1021 cctacgaagg cctgccctcc gcccggagac ccgaagtcgc cgtggtcgcc gcgtatttcg
     1081 gccaggccgg cgccctcaac cagctggaca aggccgaccg tctgcccgtc gtgcattccg
     1141 gccacatgaa ctactacctc tggtggcagg cggacgagcc gaatgcccgg ctcgaggacg
     1201 tgctcctcgt cggcttccac ccaaaggagc tcgaaccgct ctttgcggaa atcaccatcc
     1261 tcgaccgctt ctcctgcaat cgctgcatga cgcgggaaca gggcgcctat ctgattcgcg
     1321 caagacggcc gctgattgat cccgaagaga tccgcgacag gctcaagcgc ttccatttct
     1381 tttgagtcgc cgggcagatg taggcaaaat gacctgtccc agacaaggtt ttgccaaatt
     1441 gagtgaagcc agacttggcc tggagcaggc ggcgccagat agcattacga agttggacgc
     1501 gatccgccac tcggtggaag cgcgccttcg cgagctttcg ccgtccgctg ccgaaggcgg
     1561 cgagcgcatc gagcacgcca tccaatacag cctgctggac ggcggcaagc ggctcaggcc
     1621 gctgctcacc atcctgactg cttcgacatt cgacgcagac cccgatctgg cgctggatcc
     1681 agcctgcgca atcgagatgg tacataccgc ctcgctcgtc ctggatgacc tgccctgcat
     1741 ggacgacgcc cttcagcgtc gaggcaagcc ggcgacgcac gtcgcccacg gcgaggacat
     1801 cgccatcctc tcggcggttt ccctgataag cgaggcgttc ggcgtaatcg cccgggcgcc
     1861 ggggatcgcg ccggaagtgc gaacgagcat cgtcggccgc ctggcggacg cgatcggaat
     1921 ccaagggctc tctgccgggc aggagcgcga tctgcatgag gcccagagta cgaccagccg
     1981 ggccgctctc gagaagcgcc atcatcagaa aacgggcgct ctgttcgtgg cgtgcctgga
     2041 agccggcgcc cgcatcgccg gcgtggacga gaccaccatg gccgcgatga cgaaattcgg
     2101 cgagcgggtc ggcatcggtt tccagattct ggacgatctt ctggacctgt ccgcatccga
     2161 gtccaccggt aaggacgctc atcgcgacga gggcaaagcc accttcgcca ccgtgatgac
     2221 gattcaggaa gccgaacgtg aagccatcac ctgcctcgat aacgcgcttg ccgaactggc
     2281 cgaccagatc gcagacgcgg ggccgttcgc cgttttcgtg gatctggtgg ttcgcgccta
     2341 cgccgatcag gtgagcagga gggggtccga gatcgctggc gcgttgcggc cgtgaccgtc
     2401 gtcgtcaacg ccgcctgttt cctgaccgct ctcgccatca tggaatgggt ggcctgggtc
     2461 acccacaagt acgtgatgca cgggtttctc tggagcctgc acgaatcgca tcatcagccc
     2521 cgaaaggggc gcttcgagcg caacgactgg tttgccgtgt tctttgccgt accgtccatc
     2581 gtgctcatct atctcggcaa caatggctat ccggccgcgc tctggggcgg gctcggcgtc
     2641 gcggcctacg ggctgatcta cttcatcttt cacgacgtga tcgtccaccg acgcctcgac
     2701 accggttacg cgccgcgcag ccgctacatg cgtcgtatcg ttcacgcgca tcgccttcac
     2761 cacgccgtgt ccgaaaagca tggcgcggta tctttcggct tcgtctacgc gccgccgatt
     2821 cccgtattgc gggcggagat gaaagccctg gcggcctccg gggcgtcagg aagctgacca
     2881 gagcccacgt ctgatagggg ccgtccggcg tcgcccgaac gtaacctgca gtcccgctaa
     2941 cgctgctcct ttaagaagca gcacgagctt tcggcctacg ccgacggtcg cccggtgctg
     3001 ccagacgtca ggcccgtcac ggcgaacgac ccgaccaatg tcgcgataga tggcaagcgc
     3061 gctggcgatc gcccaggcat tgcgaaacct aaggcggcca atgccgtgtc ttgccgactc
     3121 gtagtagcgc tcggcctcgt cgagaatccg aatggtctga ccgtagatga cgtcgcggtg
     3181 gtcgggaaac tgcccggtgt cctcgggcac cccggcgtcg gcgagccatt gcctgggcag
     3241 gtagcagcgt ccgtcggcag cgtcctgcat gacgtcgcgg gcaatgttgg tcagttgaaa
     3301 cgcgatcccc aggtcgacgg cccgagccac cacctctgga tcacgaatcc ccatgaccgt
     3361 cgccatcatc ccgcccacgg tccctgccac gtgatagcag taacgcagcg tgtcgctgag
     3421 cgtgcggcac tcatgaccct cgacgtccat ggcgaaccca tcgagcaatt ccagcgggta
     3481 gttccgggga atgtcacatt cgagaaagac tctctgaaag gcgacgaacg gggttccgtc
     3541 gaccgcctca ccgcgcatgg ccgcttcggt atcccgccgc aactgctcca gccgcgcgcg
     3601 ctgctccgga ggtgccggag agcgctgccc gatgccgaga tcctggccgt cgatctcgtc
     3661 atcgcagtgg cggcaccacg catatagcat gatcgcgtga ccccgggtcg tcgggtcaaa
     3721 caggcgcgcg gccgcagcga agcttttcga gcccctggag atgatcgacg cgctggctgc
     3781 gaaaacgtca ctcactcagg ccatctcctt ggcgaactga tccgaccggt cgctgtccgg
     3841 atacgttcgg ccgaagtcat cgagcatcag gcgcgatgtg gccttggcgg agccgaccac
     3901 gccgggtatc cccgcgcccg ggtgcgtgcc cgctccggca aagtaaaggc ctttgagcac
     3961 gtcgtcgcgg ttgtgcgtgc ggaaccaggc gctttgccat agcacgggtt ctaaagaaaa
     4021 cgccgagcca agatgcgcgt tgagctgcgt ctgaaagtca tccggcgtga acgtgcgggt
     4081 ggtgaccaga cgctcaccga gccccgggag atagtgcttt tccaggtacg caagaatgtg
     4141 atcccggtaa cgcgggccaa gctcggacca gtcgaggtcg gccgccttga gattgggtac
     4201 cggcgacaac acgtagaagg tcgagcagcc tggcggggcg agagaggggt cggtaacgct
     4261 gggcgcgtgc agatacaacg agaaatcgtc cggcagcccg ggaccggcgt aaatttcctt
     4321 gagaagctcc cggtaacgcg gaccgaagag aatgctatgg tgcttcagtt cggggtagct
     4381 gcccctgacg ccaaagtaaa ccacgaacag cgaggggctg aatcttttgc gccgcagcgc
     4441 gcgggcggcc gctctgcccc gcgccgtccg gccaagcagc ctttcatagg tgaacaccac
     4501 gtcggcgttg ctggccacgg cgtcgaaccg tccctcccag ccgttgtccg tccggacacc
     4561 cgtgacgcgg tcgccgctgg tcaatatctc cgccaccggc gtgttgagct tgagctcgcc
     4621 tccaagatcg ccgaaaaggc ggaccagccc ctgcaccaga gcgccggttc caccctttgg
     4681 aaagtgcacg ccccagcgct gctcgaggga gtgaatcagc gtgtagatgg acgaggtgtt
     4741 gaacggattg ccgccgacca gcagcgtgtg aaagctgaac gcctgccgca gctgctcgtc
     4801 cttcacgaac cgcgacacca tgctgtagac gctgcgatag ctctgcagcc gaaccagctg
     4861 gggcgccgcc ttgatcatgc tggcgaagtc gagaaacggc acgctgccca gcttgacgta
     4921 tccctccttg aggacctcct ccgagtaagc gaggaagcgc cgatagccgt caacgtccgc
     4981 tgggttcttg gcgccgatct gcgcagcggt ctcatccgga tcgttgctgt agtcgaagga
     5041 gtagccgtcc tcccagcaga gccggtagaa cggagcgatg ggagtcagct ccaggtaatc
     5101 ctcgagcgac cggccgctca acgcaaacag ctcacgaagg cagtccgggt cggtaatgac
     5161 tgtgggcccc gcgtcaaacg tgaacccctc gtcctcgtag acgtaggccc ggccacccgg
     5221 acggtcgcgg ccctcgaaga gggtcgtgcg gaagccggcc gattgcaggc gtatggcgag
     5281 cgcaagcc
//
LOCUS       DBNHOX010000550         2524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1009030, whole genome shotgun sequence.
ACCESSION   DBNHOX010000550 DBNHOX010000000
VERSION     DBNHOX010000550.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1009030"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /gene="recN"
                     /locus_tag="AC16UZ_12135"
     CDS             complement(<1..1638)
                     /gene="recN"
                     /locus_tag="AC16UZ_12135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR6247478.1"
                     /translation="MLRELFVRDFALVESLEIGLCEGLTVITGESGAGKSILLGALGL
                     VLGDRADTDTIRPGAKRADVTAEFDLSGQPAAQAFLAEHDLEDADHAERCLVRRVVNQ
                     EGRSRAFINGAAVTRALLREFAEDLVDIHGQHEHQRLAEPATQLKLLDDYGVDAALLE
                     SCRESYRFWQRAVAEADALEAALASKQDRASLLSYQLEELAALGLEPGEFETLESEHK
                     RLSQAQDLRETVGHGLAVLEEADVLGRVSGALANLDDEHPALQAARDLLSSADSLVTD
                     AVRELRRYDDSLDVDEGQLGLLEERLTSVVDLARKHRVQPDRLVEHMALLQAELDAIS
                     TDRGSLDALREAAAGHEAEFRSTAGDVSLQRHEAAVHFASAVSGCMQNLGIGGGELRL
                     AFKEGESERGLESVEYQVMTNPKYPEAPLARIASGGERARISLAIQVVAAEKSALPCL
                     VLDEADVGIGGTTADVLGRLLRSLARHTQVICVTHAPQVAALGNHHLRVTKSSDQDTR
                     IEPLSEDTRIDEVARMLAGSGVTDKSRDYARTLLDEAH"
     gene            1735..>2524
                     /gene="hrcA"
                     /locus_tag="AC16UZ_12140"
     CDS             1735..>2524
                     /gene="hrcA"
                     /locus_tag="AC16UZ_12140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR6247479.1"
                     /translation="MARKQVEDNVDDRAQLLFKLLVERYLADGSPVASKKLAAFPEVE
                     VSPATVRNIMAELEDRGLVRSPHTSAGKVPTNLGLRFFVDSLLAVQPLGEEQIHRLEL
                     ELNPDLSPQELVESASNLLSHLTRMTCVVTLPRRDQVALRHVEFLRLSGDRVLVILVL
                     NDREVQNRVIHTDREYEDTELTQAANFINREFGGLSLVSIRDALLSSMQADKDGMDRL
                     MQTALDMAGRVFSESDQPLGKELVIAGESNLLDVSGDMATVRTLF"
BASE COUNT          420 a          824 c          837 g          443 t
ORIGIN      
        1 atgggcttcg tcgagcaggg tgcgggcata gtcccgcgac ttgtcggtga cgccagaacc
       61 cgccagcatc cgcgcgacct cgtcgattcg ggtgtcctcg gaaaggggct cgatgcgcgt
      121 gtcctgatcg ctggatttgg tcacccgcag gtgatgattg cccagggcgg cgacctgggg
      181 ggcgtgggtg acgcagatga cctgagtgtg ccgtgccaga gatctcaaga gccttccgag
      241 cacgtccgcc gtggttccgc cgatgcccac gtccgcctcg tccaggacca ggcacggcag
      301 ggccgacttc tcagcggcca ccacctggat ggcgaggctg atgcgggcac gctcgccgcc
      361 ggaggcgatc cgggcgagcg gggcctccgg gtatttgggg ttggtcatca cctgatactc
      421 cacgctctcc agcccacgct cggactcgcc ctctttgaag gccaggcgca gctcgccgcc
      481 gccgataccc agattctgca tgcaaccgct gacggccgag gcaaagtgaa cggccgcctc
      541 gtgccgctgg agagacacgt cgccggcggt tgagcggaac tcggcttcgt gtccggcggc
      601 cgcctcgcgc agggcgtcga gggagccgcg gtcggtggag atcgcgtcga gctcggcctg
      661 cagtaaggcc atgtgctcga ccagccggtc cggctgaacc cgatgcttgc gggcgaggtc
      721 gacaacgctc gtcagccgct cttccagcag cccgagctgg ccctcatcca cgtccaggga
      781 atcgtcgtat cggcgcagct cgcgtaccgc gtcggtcacg aggctgtcgg cggatgacag
      841 cagatcccgt gcagcctgaa gagccggatg ctcgtcatcc aggttggcca gcgcgcccga
      901 gacgcggccc aggacgtcgg cctcctccaa caccgcgagg ccgtggccga cggtctcgcg
      961 caggtcctgg gcctgggaga gccttttgtg ctcgctttcc agggtttcga actcccctgg
     1021 ctctagcccc agcgcggcca gctcctcgag ctggtaagag agcagtgagg cgcggtcctg
     1081 cttgcttgcc agggcggcct cgagggcgtc cgcttctgcc actgcccgct gccagaagcg
     1141 atagctctcc cggcacgact ccaggagcgc ggcgtccacg ccgtaatcgt ccaagagctt
     1201 gagctgggtg gccggctcgg ccaggcgctg gtgctcgtgc tgaccgtgaa tgtccacgag
     1261 gtcctcggcg aactcgcgca gcagagcgcg ggttaccgcg gcgccgttga tgaaggctct
     1321 ggagcgaccc tcctggttga ccacccgacg gaccaggcat ctttcggcat ggtcggcgtc
     1381 ctccaggtcg tgctcggcaa gaaacgcctg agcggcgggc tggccgctga ggtcgaactc
     1441 ggcggtgaca tccgcccgct tggcgccggg cctgatggtg tccgtgtctg cccgatcgcc
     1501 tagaaccagc cccagggcgc cgagcagtat ggacttgccc gctcccgact cgccggtgat
     1561 cacggtcagc ccctcgcaaa gaccgatctc cagcgactcc accagagcaa aatcccttac
     1621 gaagagctct ctaagcatgg cttgacgcgg gatgaattag gttcatagtg gactgtcaga
     1681 acgcctgtac cgtgggcctt cagagcgccg caccgtaatc attagccaac gaaaatggcc
     1741 agaaagcaag tagaagacaa cgtagacgac cgtgcgcagc ttctcttcaa gctgctcgtg
     1801 gagcgctatc tggcggacgg cagcccggtt gcctcgaaga agctcgccgc cttccccgag
     1861 gtggaggtga gcccggctac ggtgcgcaac atcatggccg agctagagga tcgcggtctg
     1921 gtccggtcgc cacacacgtc ggcggggaag gtgcccacga atctggggtt gcgctttttc
     1981 gtggacagcc tgctggccgt gcagcctttg ggcgaggagc agatccaccg gctcgagctg
     2041 gaactcaatc cggatctttc tccccaggag ctggtcgagt cggcgtcgaa cctgctctcc
     2101 caccttaccc gcatgacctg cgtggtgacg ctgccccgcc gcgatcaggt tgccctcagg
     2161 cacgtcgagt tcctgcgcct gtctggtgac cgggttctgg tcatcctcgt tctcaacgac
     2221 cgcgaggttc agaaccgggt aatccacacg gaccgggaat acgaggacac ggagctcacc
     2281 caggcggcca acttcatcaa tcgggagttc ggcggcctgt cgctggtgag tatccgcgac
     2341 gccctgcttt ccagcatgca ggccgacaag gacggcatgg acaggctcat gcagacggct
     2401 ctggacatgg ccggtcgcgt gttctcggaa tccgaccagc cgttgggcaa ggagctggtg
     2461 attgccggag agagcaatct gctcgacgtc agcggagaca tggcgacggt gcgcacgctg
     2521 tttg
//
LOCUS       DBNHOX010000551         2095 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1715746, whole genome shotgun sequence.
ACCESSION   DBNHOX010000551 DBNHOX010000000
VERSION     DBNHOX010000551.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1715746"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="AC16UZ_12145"
     CDS             <1..563
                     /locus_tag="AC16UZ_12145"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR6247480.1"
                     /translation="IAGIDLGAIRQEKGWTNPFTSVYGSSDGRYVLLTVTNQAREWPA
                     LCKALGHQEWLEDERFADFRTLFRNRVELIRLISEATETMTLAELTAALDAHGITYGV
                     VSQMAEVVDDPHLRAAGIIVDTASPDESYAATIANPISMAEAPKRSHGRAPEIGEHSR
                     EILREHGFTDEQIDELERCRVIGQHR"
     gene            577..1380
                     /gene="hpaH"
                     /locus_tag="AC16UZ_12150"
     CDS             577..1380
                     /gene="hpaH"
                     /locus_tag="AC16UZ_12150"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003209880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HIR6247481.1"
                     /translation="MLNPEQARAAAMELDTAERGRRQIRPTSVTFQGMTVDDAYAVQD
                     AWVKLKIDSGRRIVGRKVGLTSRAMQEAMQIAEPDFGTLLDDMMFEDGASLKAGDFLD
                     PRIEVELAFVLGQTLAGADVTLDDVLEATDYVTPALEIIAARSFRVDPQTGSPRTVVD
                     TISDNAANAGVIVGGRRVRPDELDLRWVSALLYRNGIVEETGVAAGVLDHPGNGVVWL
                     ARKFAEHGVSLERGEIVLAGSFTRPVAVRPGDEFRADYGPLGVIGCAFS"
     gene            complement(1434..>2095)
                     /locus_tag="AC16UZ_12155"
     CDS             complement(1434..>2095)
                     /locus_tag="AC16UZ_12155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HIR6247482.1"
                     /translation="VPTRSAVVTAGLNANVAYLLPIVALLGSTLLTLAFTADMDWLYP
                     LRVVMVGAVLALAWRHFRFVWAPVSWVAVGGGFLVLALWLALVPTDPAQDALVGAKIG
                     EAPLWIAALWIIFRCVGSAVTVPLAEELAFRGYLMARLTPGEPSPADRLPFSWLALLV
                     SSAVFGLLHGAWLAGAIAGLTYGLIRYHRGRVADAVVAHAVTNALLSVYVLVTGHWSL
                     W"
BASE COUNT          379 a          672 c          731 g          313 t
ORIGIN      
        1 tcatcgcggg aatcgatctc ggcgccattc gccaggagaa aggctggacc aacccgttca
       61 cctccgtcta cgggtccagc gatggtcgtt acgtgctcct gaccgtgacc aaccaggccc
      121 gcgagtggcc ggcgctgtgc aaagcgctcg gtcaccagga gtggctcgaa gacgagcggt
      181 tcgccgattt cagaacgctg ttccggaatc gggtcgagct catccggctg atcagtgagg
      241 ccacggagac catgacgctc gcagaactca cggcggccct ggatgcccat ggcatcacct
      301 acggcgtggt cagccagatg gccgaggtgg tggacgaccc tcatcttcgc gccgcgggca
      361 tcatcgtcga caccgcttct cccgacgaga gctacgccgc taccatcgcg aaccccatca
      421 gcatggccga ggcccccaaa cgctctcacg ggcgggcgcc cgagatcggc gagcactccc
      481 gtgagattct ccgcgagcac ggctttacgg acgagcagat cgacgagctg gagcgatgcc
      541 gcgtcatcgg ccagcacagg tagtgacacc gcgcccatgc tgaaccccga gcaggctcgc
      601 gcggcggcca tggaacttga caccgccgag cgcggccggc gtcagatccg gccgacttca
      661 gttacgtttc agggaatgac cgtagacgac gcctacgcgg tgcaggacgc gtgggtcaag
      721 ctgaagatcg attccggcag acgcatcgtc gggcgcaaag tcgggctcac gtcccgggcc
      781 atgcaggagg cgatgcagat tgcagagccc gatttcggca cgctgctgga cgacatgatg
      841 ttcgaggacg gcgcgagcct gaaggcgggc gactttctgg atccgcgcat cgaggtggag
      901 ctggctttcg tgctcggaca gaccctggct ggtgcggacg tcacgctgga cgacgtgctc
      961 gaggcgaccg actacgtgac cccggcgctg gagatcatcg cggcccgcag ctttcgagtg
     1021 gacccgcaga ccggttcgcc gcggacggtg gtcgatacca tctctgacaa cgcggccaac
     1081 gcgggcgtaa tcgtgggcgg acgccgtgtg aggcccgatg agctcgatct gcgctgggtg
     1141 tctgccttgc tgtaccgcaa cggaatcgtc gaggagaccg gcgtggctgc cggggtgctg
     1201 gatcatccgg gcaacggcgt ggtctggctg gcccgaaagt tcgccgagca cggcgtgtcc
     1261 ctcgagcggg gggagatcgt tctggcgggg tccttcaccc ggccggtggc cgttcgaccc
     1321 ggcgacgagt tccgggctga ctacgggccg ctgggcgtca tcggctgcgc cttctcctga
     1381 tagggccggt gtgtggcttg ttgtggcttt gtcgggggct cgaccgccgg ccgctaccag
     1441 agtgaccagt gcccggtgac gagcacgtag accgacagca gtgcgttggt gaccgcgtgg
     1501 gcgaccaccg catcggctac gcggccgcgg tgatagcgga tcagaccgta ggtgagcccg
     1561 gcaatggcgc cggccagcca ggccccgtgc agcaggccaa aaacggccga cgagacgagc
     1621 agcgccagcc aggagaaggg taagcggtcg gcgggggatg gttctccggg cgtgagccgg
     1681 gccatcagat agccgcgaaa ggccagctcc tccgcgagcg gaaccgtgac cgccgagccg
     1741 acgcagcgga atatgatcca cagcgcagct atccagagcg gggcttcgcc gatctttgcg
     1801 ccgacgagag cgtcctgggc gggatccgtg ggcacgagcg ccagccacag cgctaatacg
     1861 agaaatcccc cgcccacggc gacccaggac acgggcgccc agacgaatcg aaagtggcgc
     1921 cacgccaatg ccaggacggc gccgaccatg actacccgca gcgggtacag ccagtccata
     1981 tcggcggtga aggcgagcgt cagaagcgtc gatcccaaca gcgcgacgat gggcagcagg
     2041 taggcgacgt tcgcgttcag gcccgccgtg accacggccg atcgcgtggg tacgg
//
LOCUS       DBNHOX010000552         5477 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1779304, whole genome shotgun sequence.
ACCESSION   DBNHOX010000552 DBNHOX010000000
VERSION     DBNHOX010000552.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1779304"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1827)
                     /locus_tag="AC16UZ_12160"
     CDS             complement(<1..1827)
                     /locus_tag="AC16UZ_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6247483.1"
                     /translation="MEKLLIANRGEIAVRIARAAAELGLVTVAVYAEDDAASLHRSRA
                     DESEALTGSGPAAYLDIGQMVEVACRSGCDAVHPGYGFLSENPDFAAAVEAAGLTFVG
                     PTPETLALLGDKVSARRLAGTCGVPLLAGSQAPVTLEEARAFLAGLPDGVDMVIKAVA
                     GGGGRGMRVVGSAADLEEAFARCCSEARLAFGDDRVYVEEFLPKARHLEVQVLGDGTG
                     AVSHVWERECSIQRRHQKIVEVAPSPTIGPQLRESLLRAAVDIGEAARYRGLGTIEFV
                     VDGSGERYAFIEANARLQVEHTVTEEVTGLDLVRAQLEVAAGRTLAELSLEQRDIPVP
                     RGYAAQARINMETMLADGTTRPGGGTLTSFEVPTGPGLRTDTFGYVGYTTSPAYDSLL
                     AKLIVHSPAGSFADCARKGYRALCETRIEGVATNLDFLRNLFAQPAFQASEVHTGYVD
                     EHVDELVKEAGHRVYHGGRAAAAPATPVLAGVKVDAADPLAVLDHGKSGGASVAAAPR
                     AIALVDEELPAGVSVIRAPMQGTVVSFEVAPGDEVYVGRQVLVMDAMKMEHVVKAEVS
                     GIVQRLGVNEGDTVFEGHPLVYLQEADIAAGEEAGQEAFDLDY"
     gene            2004..2708
                     /locus_tag="AC16UZ_12165"
     CDS             2004..2708
                     /locus_tag="AC16UZ_12165"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6247484.1"
                     /translation="MTVTLSASGLTKVYQTGDVVVHALRGAELELREGELVVMLGPSG
                     SGKSTLLNILGGLDVPTAGTVWFRNRQLTASDEASLTEYRRRHVGFVFQFYNLIPSLT
                     ARENVALVTEISSNPMDAAEALALVGLAERLDHFPAQLSGGEQQRVAIARAIAKRPEI
                     LLCDEPTGALDSETGILVLAAIDQVNRELGTTTAVITHNTVIGAMADRVMHMADGLIA
                     RVDENPDRQPPDELSW"
     gene            2732..5077
                     /locus_tag="AC16UZ_12170"
     CDS             2732..5077
                     /locus_tag="AC16UZ_12170"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6247485.1"
                     /translation="MLRDLVHMKGQVIAVGLVIASGVAVLVMALSTLEALRETTDAYY
                     ERYRLADVFAGVKRAPEPLSDRIREIEGVQTVQTRIVKFATLDVLHFPEPVMGQFVSL
                     PESGPPLLNQLALRAGRHVEPGQHDEVVVNEPFAEAHGLTPGDSLVAVLNGKRRLLRV
                     TGIALSPEFIYSLGPGALMPDDERFGVIWMNRKALAAAFDLKDAFNNISLQLTRGTRP
                     EGVITQLDRLLEQYGGIGAVARANQMSNWFVMNEIDQLATISRILPSIFLLVSAFLTN
                     MVLTRLIDTERAQIGLMKAFGYTSFEVGFHYTKLVLGMAVVGIVVGLFVGGWFGRLNT
                     QMYAEVFRFPLLLYRPSPSAFAIASALSLLAALAGAMSSVRRATRLPPAQAMQPPAPP
                     TFRRGRLAHSRFGRWLDQPTRIVLRNIARWPGRAALTTTGIAASVALLVLALQWNDSL
                     NYLAQSYFFQAQRQHVMIGLADTRSATVMHDIEQLPGVLAAEPMRIVSADLSRGAVTH
                     RGALTGVADGAQLQPIYDDARGVEIPTPPEGLVLGITLAEKLGVGVGDRIWVNVLEGR
                     RPNVQLPVVEVVETYIAMPAYIHIDALNRLLKERPSVEYANLRIDRLEESRLYAELKK
                     LPVVSAVMLRQAAIDAFYDNVVEHLMVFITMFSSLACILGFGVAYNSVRIALSERGRE
                     LATLRVLGFTRGETSYILLGEVALLIVAALPLGCLLGRLLSQLIAAAFNTELFRVPLT
                     IDPSTYGIAVLIALAATTLSAALVRRRVDRLNMIEVLKTRE"
     gene            5080..>5477
                     /locus_tag="AC16UZ_12175"
     CDS             5080..>5477
                     /locus_tag="AC16UZ_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6247486.1"
                     /translation="MNANAKRILVWSVVAIAVAVGLTIAFMPRPVTVDLITVIPAPME
                     VTADEEGETRVHDVFVLSAPVAGRLRRIDAHVGDPVLADETVLARIEPADPTFLDPRT
                     EAQARAARQAAVSALTLAKARVEEARAEFE"
BASE COUNT          895 a         1906 c         1770 g          906 t
ORIGIN      
        1 gtagtccaga tcgaacgctt cctggccggc ctcctcgccc gcggcgatgt ctgcttcctg
       61 cagatagacg agggggtggc cctcgaagac cgtgtcgcct tcattcacgc ccaggcgctg
      121 gacgatgccg ctgacttccg ccttgaccac gtgctccatc ttcatggcgt ccatgaccaa
      181 cacctgcctg ccgacgtaga cctcatcgcc gggtgccacc tcgaagctga cgacggttcc
      241 ctgcatgggc gcgcggatga ccgaaacccc cgcgggcagc tcttcgtcca ccagcgcgat
      301 ggcccgcggg gccgctgcga cgctcgcccc gccagactta ccgtgatcga gaacggcgag
      361 cggatccgcg gcgtccacct ttacgccggc cagcacgggc gtggccggcg cagcggcagc
      421 gcgtcctcca tgatagaccc tgtggcccgc ttctttgaca agctcgtcca cgtgctcgtc
      481 cacgtagccc gtgtgcacct cgctggcctg gaaggcgggc tgggcgaaga gattgcggag
      541 gaagtccaga ttggtcgcca ccccctcgat gcgggtctcg cagagggccc gatagccttt
      601 gcgggcacag tcggcaaagc tgcctgcggg ggagtgaacg atgagcttgg ccaggagcga
      661 gtcataggcc gggctcgtgg tgtagccgac ataaccgaag gtgtccgtac gcagaccggg
      721 gccggtaggc acctcgaagc tcgtcagcgt gccgccaccg ggcctggtgg tgccgtccgc
      781 tagcatggtc tccatgttga tccgcgcctg ggccgcgtag cccctgggca ctgggatgtc
      841 gcgctgctcg agggagagct cggccagggt gcggccggcg gcaacttcca gctgggcgcg
      901 aacgaggtcc aacccggtca cttcctcggt gaccgtgtgc tcgacctgca gccgcgcgtt
      961 ggcttcaatg aaggcgtagc gctcaccgga accgtcaacg acgaactcga tggtgccgag
     1021 gccccgataa cgtgcggcct ccccgatgtc cacggcggcg cgcagcagcg actcgcgcag
     1081 ctgaggaccg atcgtcgggc tgggcgccac ctccacgatc ttctgatggc gccgctgtat
     1141 gctgcattcg cgctcccaga cgtggctcac ggcgccggtg ccgtcgccca gcacctgcac
     1201 ctccaggtgg cgggctttgg gaaggaattc ctctacgtag acgcgatcgt caccaaaggc
     1261 gagcctggct tccgagcagc agcgcgcgaa agcctcttcc agatcggcag cgctgccgac
     1321 cacccgcatg ccccggccac cgccgccggc gacggccttg atcaccatgt ccacgccgtc
     1381 cgggaggccg gcaaggaagg cgcgggcctc ctcgagcgta accggcgctt ggctgcccgc
     1441 cagcagcggc acgccgcagg tgccggcaag ccggcgcgcg ctcaccttgt caccgagcag
     1501 cgcgagggtt tccggagtcg ggccgacgaa ggtgagaccg gccgcctcca cggctgcggc
     1561 aaaatcgggg ttctcgctca aaaagccgta gccaggatgg acggcgtcgc atcccgaccg
     1621 gcacgccacc tccaccatct ggccaatgtc caggtaggcc gcggggccgc tgcccgtgag
     1681 cgcttcggat tcgtccgccc gggagcggtg cagggaggca gcatcgtcct cggcataaac
     1741 ggcaacggtg accaggccga gctcggcagc cgcacgggcg atacgaacgg ctatctcgcc
     1801 ccgattggca atcagcagtt tctccaagct ccctcctcga ccgttcccgt gagatgggcg
     1861 gccagctccc cgaaaggcag aaaaacgatc ggcgattgtc tgacgttgat cagtcgaagt
     1921 ccaacgggca acctcgtgaa agtgcggatg gtggttctgc ccgcagaatc atctaatctg
     1981 ggcttcccgg cgaaggcgaa accatgactg tcaccttgag cgcaagcggt ctcaccaagg
     2041 tctaccagac cggggacgtc gtcgtgcacg cgctgcgcgg ggccgagctc gagctgcgcg
     2101 aaggtgagct ggtggtgatg ctgggccctt cggggtcggg gaagtccacg ctgctgaaca
     2161 tcctcggcgg tctggacgtg cccacggccg ggacggtctg gttccgcaat cgccagctga
     2221 cggcctccga cgaggcctct ctcacagagt accggcgccg gcacgtgggc ttcgtcttcc
     2281 agttctacaa cctgattccc agcctgaccg cccgggaaaa cgtcgccctg gtcaccgaga
     2341 tctcctcgaa ccccatggac gctgcggagg ccctcgcgct ggtcggcctc gccgagcgtc
     2401 tggatcactt cccggcccag ctctccggag gcgagcagca gcgtgtggcc atcgctcggg
     2461 cgatcgcgaa gcgccccgag atcctgctct gcgacgagcc gaccggggcc ctggacagtg
     2521 aaaccggcat cctcgtgctg gccgccatcg accaggtcaa ccgtgagctc ggcaccacca
     2581 cggccgtgat cacccacaat accgtcatcg gtgccatggc cgaccgggtc atgcacatgg
     2641 cggacgggct gatcgcccgc gtcgacgaga atccggaccg ccagcccccc gacgagctga
     2701 gctggtaact gtgcgggcgc tcgatacgaa ggtgctccgc gacctcgtcc acatgaaagg
     2761 acaggtcatc gccgtcggcc tggtcatcgc atccggggtg gcggtgctgg tgatggccct
     2821 gtccaccctc gaggcccttc gcgagaccac cgacgcctac tacgagcgtt accgcctggc
     2881 ggacgtgttc gccggggtca aacgggcgcc ggagcctttg tcagacagga ttcgcgagat
     2941 cgaaggcgtg cagacggtgc agacccgcat cgtcaagttc gcaacgctgg acgtcctcca
     3001 cttcccggaa ccggtgatgg gccagttcgt ttccttaccc gaaagcggcc cgccgctgct
     3061 gaaccagctc gcgctgcgtg ccggaaggca tgtcgagcca ggccagcatg acgaggtggt
     3121 cgtcaacgag ccgttcgccg aagctcatgg gctcacgccc ggcgactccc tggtggcggt
     3181 gctgaacgga aagcgccgct tgctccgcgt gacggggatc gctctctctc ccgagttcat
     3241 ctacagcctg gggcccggcg ctctgatgcc cgacgacgag cgcttcggcg tgatctggat
     3301 gaaccgcaag gcgctcgccg ccgccttcga cctcaaggac gcgttcaaca acatctccct
     3361 gcagctcacc cgcggaacca ggccggaagg cgtgatcacg cagctcgatc ggctgctcga
     3421 gcagtacggg ggcatcggcg ccgtcgcccg cgccaaccag atgtcgaact ggttcgtcat
     3481 gaacgagatc gaccagctgg ccacgatatc gcgcattctt ccgagcatat ttctgctggt
     3541 ctccgccttc ctgaccaaca tggtgctcac ccggctgatc gacacggaaa gagcccagat
     3601 cgggctgatg aaggccttcg gatacacgag tttcgaggtg ggctttcact acacgaagct
     3661 ggtgctgggc atggccgtgg tgggcatcgt cgtgggcctg ttcgtcggcg gctggttcgg
     3721 ccgtctcaac acgcagatgt acgccgaagt attccgcttc cccctgctcc tctatcggcc
     3781 gagcccgagc gcgttcgcca tcgcgtcggc attgagcctg ctggcagcgc tcgcaggtgc
     3841 catgagcagc gtccgccggg cgacccgtct gcccccggcc caggccatgc agccgccggc
     3901 gccacccacg tttcgacggg gacggctcgc ccactcgcgc ttcggccgct ggctggacca
     3961 gccaacccgc atcgttctgc gcaacatcgc ccgttggccc ggccgcgccg cgttgaccac
     4021 caccgggatc gccgcttcgg tcgccctgct ggtcctcgcg ctgcagtgga acgactccct
     4081 gaactacctg gcgcagagct acttctttca ggcccagcgc cagcacgtga tgatcgggct
     4141 tgcggatacc cggtccgcca cggtgatgca cgacatcgag cagctcccgg gggtgctggc
     4201 agcagagccc atgcgcatcg tcagcgcaga cctatcccga ggagcggtaa cccaccgcgg
     4261 cgcgctgacg ggcgtcgccg acggcgcgca gctgcagccg atctacgacg acgcgcgggg
     4321 cgtggagatt ccgacgccgc ccgaaggcct ggtgctgggc atcaccctgg cggagaagct
     4381 gggcgtgggc gtgggcgacc ggatctgggt aaacgtgctc gagggacggc ggcccaacgt
     4441 tcagctgccc gtcgtcgagg tggtggaaac ctacatcgcc atgccggcct acatccacat
     4501 cgacgcgctc aaccgcctgc tcaaggaacg cccgagcgtg gagtacgcga acctgcgcat
     4561 cgaccgcctc gaagagtcca ggctgtacgc cgagctcaag aagctgcccg tggtctctgc
     4621 ggtgatgctg cgccaggcgg ccatcgacgc cttctacgac aacgtggtgg agcatctgat
     4681 ggtgttcatc accatgtttt ccagcctcgc ctgcatactc gggttcggtg tcgcctacaa
     4741 cagcgtgcgt atcgctctgt cggaaagggg gcgcgagctg gctaccctgc gggtgctcgg
     4801 cttcacccga ggggagacgt cctacatcct cctgggcgag gtggcgctgc tcatcgtggc
     4861 cgccctgccg ctgggctgcc tgctcggccg actcctctcc cagctgatcg ccgccgcctt
     4921 caataccgag ctgttccgcg tgcccctgac catcgatccg tccacgtacg gcatagccgt
     4981 gctcatcgcg ctggcggcca cgacgctgtc cgcggccctc gttcgccgac gcgtcgaccg
     5041 cctgaacatg atagaagtgc tcaagacccg ggagtagaaa tgaacgccaa cgccaagcgg
     5101 atactggtct ggtccgtcgt cgccatcgcc gtggccgtcg gcctgacgat cgcattcatg
     5161 cccaggccgg tgaccgtgga cctgatcacg gtcattccgg cgcccatgga ggtcaccgcc
     5221 gacgaggaag gtgagacgcg cgttcacgac gtcttcgtgc tgtccgctcc cgtcgccggc
     5281 cggctgcgcc gcatcgatgc ccatgtcggc gacccggtcc ttgccgacga aaccgtcctg
     5341 gcccgcatcg agccggccga tccgaccttt ctcgacccgc gcacggaggc ccaggcccgc
     5401 gccgcgcggc aggcggcagt ctctgcgctc accctggcaa aggcgcgagt cgaggaagcg
     5461 cgggcagagt tcgagta
//
LOCUS       DBNHOX010000553         1972 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_558735, whole genome shotgun sequence.
ACCESSION   DBNHOX010000553 DBNHOX010000000
VERSION     DBNHOX010000553.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_558735"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="AC16UZ_12180"
     CDS             <1..963
                     /locus_tag="AC16UZ_12180"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6247487.1"
                     /translation="GHTTFFRNANRGKQSVVMNLKDPAARESLLQLCEGADVFIESFR
                     PGVVRRLGVDYEAVAARNPRIVYASLSAFGQFGPNRDLPAHDLAVEALSGVLSVNLGS
                     DGQPAMPGVPSADMAASLMAFGGIMMALYRQAKTGRGDYLDISMQDSLMAWLPNVLGP
                     VFVENRAPVPQDERTWGGAAFYRIYRTRDGRHVVLGAQELKFVRHLLGEFGRPELVEL
                     AERGPGPHQKPLVDFLQARFETRTQAEWVEWFRGRDIGFAPVRDLREAFDDAHVRERG
                     MLLTDAEGIQHLGVPMRYRDEPAKPSFHVPELGENDDIMASPHR"
     gene            complement(1187..1669)
                     /gene="slyD"
                     /locus_tag="AC16UZ_12185"
     CDS             complement(1187..1669)
                     /gene="slyD"
                     /locus_tag="AC16UZ_12185"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008085027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6247488.1"
                     /translation="MKIAHEKVVSIHYTLTNQEGTVLDSSSGSEPLAYLHGFGNIIPG
                     LENALEGKETGEKLSVTVEPEQGYGARDEQLVQAVPRSAFKGVEELAPGMQFQAQGPQ
                     GTRLVVVTQVAQDVVTVDANHPLAGQTLHFEVEVSEVRAATAEEIEHGHVHGPHGHHH
                     "
     gene            complement(1784..>1972)
                     /locus_tag="AC16UZ_12190"
     CDS             complement(1784..>1972)
                     /locus_tag="AC16UZ_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247489.1"
                     /translation="HGNGPRRDAPGRCADVRLQAEGSLSQINQWAAPGPTRVGMFRLE
                     NRDVEAITTARPIEFLPA"
BASE COUNT          313 a          673 c          652 g          334 t
ORIGIN      
        1 gggcacacga cgttcttccg caacgccaac cgcggcaagc agagcgtggt gatgaacctg
       61 aaggacccgg ccgcacgcga atcgctgctg cagctctgcg aaggcgcgga cgtgttcatc
      121 gagtccttcc ggcccggcgt cgtgcggcga ctcggcgtgg actacgaggc cgtcgcagcc
      181 cgcaacccgc ggatcgtcta cgcgtccctc tcggccttcg gccagttcgg tccgaatcgg
      241 gacctccctg cccacgacct ggcggtggaa gcactctcgg gcgtcctcag cgtcaacctg
      301 ggaagcgacg ggcaacccgc gatgccaggc gtcccgtcgg ccgacatggc cgcgtcgctg
      361 atggcgttcg gcgggatcat gatggcgctt tatcgccagg cgaagacggg gcgcggagac
      421 tacctcgaca tatcgatgca ggactcgctc atggcgtggc tgccgaacgt gctgggcccc
      481 gtattcgtcg aaaatcgggc gccggtcccg caggacgagc ggacctgggg cggcgctgcg
      541 ttctacagga tctaccggac gcgcgacggc cggcatgtgg tgctcggcgc ccaggaactg
      601 aagttcgtgc gccacctgct gggcgaattc ggccgccccg agctggtgga actcgccgag
      661 cgtgggcccg ggccgcacca gaagccgctg gtcgatttcc tgcaggcgcg cttcgagacg
      721 cgcacacagg cggagtgggt cgaatggttc cggggtcgcg acatcggctt tgcgccggtc
      781 cgggatcttc gcgaggcctt cgacgacgcc catgtccggg aacggggcat gctgctcacc
      841 gatgccgagg gcatccagca tctcggagtc ccgatgcgtt accgggacga gcccgcgaag
      901 ccctcgttcc acgtccccga gctgggcgag aacgacgaca tcatggcctc cccgcatcgc
      961 tgatctccac ggcggcaatc aagcgcacag caagcctgga gcacgtaccc cacggatgtc
     1021 ccgcgaaccg ggctgtgccc tgcccctggc gctgcgtcgt tcgtacgaca gagcggtcac
     1081 ggtgctggaa ggccatgtcg ggcgcctgcc atcagtcaat gacggcatcc agggcaacaa
     1141 tggccttcat cttcccggcc gtcgcgaaac ggccggcgag acccgatcag tggtggtggc
     1201 cgtgcggacc gtgcacgtgg ccgtgctcga tttcctcggc agtcgccgcc cgcacctccg
     1261 acacctcgac ctcgaagtgc agggtctggc cggccagtgg atggttggcg tccacggtca
     1321 ccacgtcctg ggccacctgc gtgacgacga ccagtctcgt cccttgcggt ccctgggcct
     1381 ggaactgcat gcccggcgcg agttcttcca cgcccttgaa ggcgctgcgg ggcacggcct
     1441 gcacgagctg ctcgtcacgg gcgccgtagc cctgctcggg ctccacggtc accgacagct
     1501 tctcccccgt ctccttgcct tcgagcgcat tctcgaggcc gggaatgatg ttgccgaaac
     1561 cgtgcaggta ggccagcggc tcgcttccag aggacgagtc caggaccgtt ccttcctggt
     1621 tcgtcagcgt gtagtggatc gacacgacct tttcgtgggc aatcttcatt cggcgctccg
     1681 cagttcccgt aaaagatcta tggtatcgcc tgaggccacc agggtcacgg aaaagggcag
     1741 aaatcggctg aaaatgacca cgggaggtgc cgcgcacccg gcatcacgcc ggcaggaact
     1801 cgatcggcct ggccgtcgtg atcgcctcga cgtcccggtt ctctaagcgg aacatcccca
     1861 cgcgcgtcgg gccgggggcg gcccactggt tgatctgcga caggctgccc tcggcttgta
     1921 gccggacatc ggcgcatcga cccggcgcgt ctcgtcgagg accgtttccg tg
//
LOCUS       DBNHOX010000554         3324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1716025, whole genome shotgun sequence.
ACCESSION   DBNHOX010000554 DBNHOX010000000
VERSION     DBNHOX010000554.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1716025"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1929
                     /gene="cysN"
                     /locus_tag="AC16UZ_12195"
     CDS             22..1929
                     /gene="cysN"
                     /locus_tag="AC16UZ_12195"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008019847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HIR6247490.1"
                     /translation="MAEAEVIQEDLQERVDAYIANQARLDLLRFITCGSVDDGKSTLI
                     GRMLYEAQLIFEDQVTSLKADSKRQGTQGGDIDFALLVDGLAAEREQGITIDVAYRFF
                     NTDRRKFIVADTPGHEQYTRNMVTGASTADVAVILIDARKGVLTQTRRHSFLAQLIGI
                     RQAVLAVNKMDLVDYDRETFETITAAYEQFAEQLAFESITAIPLSALAGDNVIERSQR
                     TSWYSGPTLLGFLETVEVGRSQREHDLRFPVQWVNRPHSEFRGYSGTVVAGAVAVGDS
                     VRVLPSGEVAQVDGIVLHEDSLDQAVADQAVTITLDRELDVSRGDVIVAADAPCEVSD
                     QFDASLVWMDQETGFIGRSYWLLMGTSRVNATLHDVKFKYNINTLEELPTRAFELNEI
                     GRVTLSLDKAVPFEAYQDCHSMGAFVLVDRYTHATVAAGMINFALRRARNIHRQAVTV
                     DKQARAKLNGHPPRVLWFTGLSGSGKSSITNAVEQVLHLQGVRTYILDGDNVRHGLNR
                     DLGFTETDRIENIRRIAEVAKLMVDAGVVVLTAFISPFRAERDMARSLFDKGEFLEIF
                     LDVPLEAAESRDPKGLYRKARRGELPNFTGIDSPYESPETADLTLNTAELSVDDCVSR
                     VLELLKDQGVS"
     gene            1926..2174
                     /locus_tag="AC16UZ_12200"
     CDS             1926..2174
                     /locus_tag="AC16UZ_12200"
                     /inference="COORDINATES: protein motif:HMM:NF021362.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HIR6247491.1"
                     /translation="MTVSDLPQTESHLRSVAKAFSWRVVATLTTMTIAWMITGEVDTA
                     IMIGGFEFVLKIFIYYLHERAWQLVPRGAVRRIARLSS"
     gene            complement(2164..3138)
                     /locus_tag="AC16UZ_12205"
     CDS             complement(2164..3138)
                     /locus_tag="AC16UZ_12205"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6247492.1"
                     /translation="MTSNRFPDPAMIFTNGIRMTVYQDGPADGFPVVLCHGFPELAYS
                     WRHQLQALADAGFHAIAPDQRGYGRTDKPDDAGAYDIHHLTGDLAGLLDAMGLERAVF
                     CGHDWGGLVVWQMALLHPSRVAGVIGAGVPFLPRAEEDPVETFRRMWGERMYIVNFQT
                     SHEADELFDADPERVFQVLMRKNPMSLAEYAELPLQERVADLIARVKAGPSSTPSIIS
                     EEELRVFASAFRAGGFTGPINWYRNWSRNWSTTAHVPQKIEVPALYIKAGNDLATAAI
                     PDLESRMAGHVADLEIREVPESGHWLQQEFPDEVSGFTVDWLTRRFTS"
BASE COUNT          599 a         1064 c         1085 g          576 t
ORIGIN      
        1 gaaacaggag ggctatttct gatggccgaa gcagaagtta ttcaggaaga cctgcaggag
       61 cgggtcgacg cctacatcgc aaaccaggcg cgcctcgatc tgctccgctt catcacctgc
      121 ggcagcgtcg acgacggcaa gagcacgctc atcggccgca tgctctacga ggcccagctc
      181 atcttcgagg atcaggtgac ctcgctgaaa gccgactcca agcgccaggg gacccagggc
      241 ggcgacatcg acttcgccct gctggtggac ggccttgcgg cggagcggga gcagggcatc
      301 accatcgacg tggcctaccg cttcttcaat accgaccggc gcaagttcat cgtggcggac
      361 acgccgggcc acgagcagta cacgcgcaac atggtcaccg gcgcgtcgac ggccgacgtg
      421 gccgtgatcc tgatcgacgc gcgcaagggc gtgctgacgc agacgcgccg ccacagcttc
      481 ctggcgcagc tgatcggcat ccgccaggcg gtgctggcgg tgaacaagat ggacctcgtg
      541 gactacgacc gggagacctt cgaaaccatc accgcagcat acgagcagtt tgcggagcag
      601 cttgccttcg agtcgatcac cgccattccg ctgtccgcgc ttgcgggcga caacgtgatc
      661 gagcggtcgc agcggacgtc ctggtacagc ggcccgacgc tgctgggttt tctggaaacc
      721 gtggaagtgg ggcgctctca gcgtgagcac gacctgcgtt ttcccgtgca gtgggtgaac
      781 cggccgcact cggagtttcg cggttactcc ggcacggtgg tggcgggcgc ggtggccgtc
      841 ggcgactccg tgcgggtgct gccatccgga gaggtggcac aggtcgacgg catcgtcctg
      901 cacgaggatt ccctggacca ggccgtggcc gatcaggccg tgaccattac tctggaccgg
      961 gagctcgacg tttcccgggg cgacgtgatc gtggccgcgg atgcgccatg cgaggtctcg
     1021 gatcagttcg atgcgtccct ggtgtggatg gatcaggaaa cgggattcat cggacgttcc
     1081 tactggttgc tgatgggaac ctcacgggtg aacgccacgc tgcacgacgt gaagttcaag
     1141 tacaacatca acaccctcga ggagctgccg acccgggctt tcgagctcaa cgagatcggt
     1201 cgggtaacgc tcagcctgga caaggccgtt cccttcgagg cttaccagga ctgtcactcc
     1261 atgggcgcgt tcgtgctcgt cgatcggtac acgcacgcca ccgtcgccgc cgggatgatc
     1321 aacttcgccc tgcgtcgggc tcgcaacatt caccgacagg ccgtgacggt cgacaagcag
     1381 gcgagggcga agctcaacgg ccacccgccc cgcgtcctct ggttcacggg cttgagcggt
     1441 tccgggaaat ccagcatcac caatgcggtg gagcaggtcc tgcacctgca gggggtgcgc
     1501 acgtacatcc tcgacgggga caatgtccgc cacgggctga accgggatct cggcttcacc
     1561 gaaaccgacc ggatcgagaa catccgccgg attgccgagg tcgccaagct catggtggac
     1621 gcgggcgtcg tggtgctcac cgcgttcatc tcgcccttcc gggcggagcg ggacatggcc
     1681 cggtcactgt tcgataaggg cgagttcctg gagatcttcc tggacgttcc gctggaagcg
     1741 gcggagtcgc gcgaccccaa aggcctctac cgtaaagccc gtcgcggaga gttgccgaac
     1801 ttcaccggca tagacagccc ttacgagtcg ccggaaacgg ccgacctcac gctcaacacg
     1861 gccgagctca gcgtggacga ttgcgtctcg cgggtcctcg agcttctcaa ggatcagggg
     1921 gtttcatgac agtgtccgac ctaccgcaaa ccgagtcgca cctgcgcagc gtcgccaagg
     1981 ctttttcctg gcgggtggtg gcgacgctca ccaccatgac catcgcctgg atgatcaccg
     2041 gcgaggtgga caccgccatc atgatcggcg gcttcgagtt cgtgctgaag atcttcatct
     2101 actacctgca cgagcgcgcg tggcagctgg tgccgcgagg ggcggtgcgc aggatcgcgc
     2161 gtctcagctc gtgaagcgcc gggtcaacca atccaccgtg aaaccgctca cctcgtccgg
     2221 aaattcctgc tgaagccagt gcccgctctc gggcacttcg cggatctcca ggtcggcaac
     2281 gtgcccggcc atgcgtgact ccaggtccgg gatggcggcg gtcgccagat cgttgccggc
     2341 cttgatgtag agtgcgggca cctcgatctt ctgcggcacg tgggcggtcg ttgaccagtt
     2401 gcggctccag ttgcggtacc agttgatcgg cccggtgaac ccgcctgccc ggaaagcgga
     2461 cgcgaagacg cggagctcct cctcggagat gatgctgggc gtgctcgacg gtccagcttt
     2521 cacccgcgcg atcaggtcgg ccactcgctc ctggagcgga agctcggcgt attcggccag
     2581 gctcatcggg ttcttgcgca tcagaacctg gaagacgcgc tccggatccg cgtcgaacag
     2641 ctcgtctgcc tcgtggctcg tctggaaatt cacgatgtac atgcgttcgc cccacatgcg
     2701 tcggaacgtt tccacggggt cctcttccgc cctcggaaga aacggtactc cggcgccaat
     2761 gacgccggcg acgcgggacg ggtgcagaag cgccatctgc cagaccacca gcccacccca
     2821 gtcgtgcccg cagaacacgg cacgctccag ccccatggca tcgagcaggc cggccagatc
     2881 gccggtcagg tggtggatgt cgtacgcccc cgcgtcatcg ggcttgtccg tgcggccgta
     2941 tccccgctgg tcgggcgcga tcgcgtggaa gcccgcgtcg gccagcgctt ggagctgatg
     3001 acgccaggag taagcgagtt ccggaaagcc gtggcagagc accaccggga agccgtccgc
     3061 aggaccgtcc tggtagacgg tcatgcgaat gccgttcgtg aagatcatcg cgggatcggg
     3121 aaaccggttg ctcgtcacgg atcatctctg ctgtcagcgc tgcgaaccca accatcgcgc
     3181 gagacagggc caggcgcaag gggcgttcgt gagggtaaga tgacgacccg gaacgacgat
     3241 tggcacgaaa tgatggttcc ttttccagcg ccccgattcg aaagcgtcaa cggcattcgt
     3301 atcgcctgct acgagatggg ggag
//
LOCUS       DBNHOX010000555         2239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_751617, whole genome shotgun sequence.
ACCESSION   DBNHOX010000555 DBNHOX010000000
VERSION     DBNHOX010000555.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_751617"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1653
                     /locus_tag="AC16UZ_12210"
     CDS             <1..1653
                     /locus_tag="AC16UZ_12210"
                     /inference="COORDINATES: protein motif:HMM:NF019042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprO/OprP family phosphate-selective porin"
                     /protein_id="HIR6247493.1"
                     /translation="GGNMRNSILIATLLLAWWPLWGSAESEEKDADLVLRNVILPDPG
                     DASADVVINVVIKDGKVLLVTKDEPPGGEGPVLDAAGTYLLGRLHTGSAPSFLLLNAD
                     PREEFSVLLDTATHAVFAISDGVVMKNDLPVPAASPDAPQRRSGWLAYTPPPISLPTS
                     YRDTSKWNRWETPFISGLFVGAVVLDRQFWQSQDAASESQVGDLTPFDGGEIRGLRFG
                     AIGTLNFPKPWVYTIFGATNAFDKGFNADDTDDVTLFDLRLDIPLFGSTLSLGKQKEP
                     ISMERTMGLVYLPIQERSAAMDGMLPARNTGLVLSGNAFAQRVSWAGGVFNDWLDTAG
                     SFDEGANQVVGRLTWLAASSADDSNLLHLGAGVRYSDAREGFRFSTEPEFNQAPLYID
                     TSIDGTLLAAEDLLTWNLEASWRYGPLWLAAEYVKTDVDAPQYADPSFSGHHITASWA
                     LTGEMRSYRRKNGTFSPLPVARSVHNNGPGAWELAARWSVFDANSGPISGGKTDIFSL
                     GLNWWLTPLFSVGANYRWITLDRDGLTGESDGFSTRILLMLE"
     gene            1712..>2239
                     /locus_tag="AC16UZ_12215"
     CDS             1712..>2239
                     /locus_tag="AC16UZ_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247494.1"
                     /translation="MAILIKLMSRIAILGLIAAPAAQAETWRPVSEPADLASLFSDTT
                     MTTTLRDGVTATAEYRADGTGTVRAWGDAFERRWSVSADSEVCIEAGDQTFCYTVETD
                     ADATDRYRSTNLETGERLILMIARQGGADPEPTQGTDSGAAGKPSAEEVAAKLANPNT
                     PLASLTTKLQYRTFDG"
BASE COUNT          426 a          738 c          718 g          357 t
ORIGIN      
        1 ggggggaata tgcgcaactc gatcttgatc gccacgctgc tgctcgcctg gtggccccta
       61 tggggcagcg ccgagagcga agaaaaggac gcggacctcg tgctgcgaaa cgtgattctg
      121 cccgacccgg gcgacgcatc ggcagacgtg gtgatcaacg tcgtgatcaa agacggaaag
      181 gtccttctcg tcaccaagga tgagcccccc ggcggggaag gcccggtgct cgacgccgcc
      241 ggcacctatc tgcttggcag gctgcatacg ggcagcgcgc cgagcttcct cctgctgaac
      301 gcggatccca gagaggaatt cagcgtgctg ctggacaccg ccacccatgc cgttttcgcc
      361 atcagcgacg gggtcgtcat gaagaacgac ctgccggtcc cggcagcatc gcccgacgcg
      421 cctcaacggc gctccggttg gctcgcctac acaccaccgc ccatctcgct gccgacgagc
      481 taccgggata ccagcaagtg gaaccggtgg gaaacgccct tcatctcggg cctgttcgtg
      541 ggcgctgtgg ttctcgatcg ccagttctgg cagtcccagg atgcggccag cgaaagccag
      601 gtcggcgatc tgacaccctt tgacggcggc gagatccgcg gcctgcggtt cggcgccatc
      661 ggcacgctga acttccccaa accgtgggta tacacgatct tcggcgccac caacgcgttc
      721 gacaaaggct tcaacgccga cgataccgac gacgtgaccc tgttcgacct gaggctggac
      781 attccgctgt tcggcagcac gttgagtctc ggcaagcaga aagagcccat ctccatggag
      841 cgaaccatgg ggctcgtcta cctgcccatt caggaacgtt ccgccgccat ggacggcatg
      901 ctgccggctc gcaacacggg gctggtgctg agcggcaacg ccttcgccca gcgcgtcagc
      961 tgggcggggg gcgtattcaa cgactggttg gacaccgcgg gctcgttcga cgagggagcg
     1021 aaccaggtgg tcggccggct gacctggctg gcggcctctt ccgccgatga cagcaaccta
     1081 cttcacctgg gcgcaggcgt gcgctacagc gacgccaggg aaggatttcg cttctccacc
     1141 gagccggagt tcaaccaggc gcccctgtac atcgatacga gcatcgacgg aactctactg
     1201 gccgcggagg acctgctgac ctggaatctg gaagcatcct ggcggtacgg gcctctgtgg
     1261 ctcgccgccg agtacgtcaa aaccgacgtg gacgcaccgc aatacgccga tcccagtttc
     1321 tccgggcatc acatcaccgc atcctgggct ctgaccggcg agatgcgctc gtatcggcgc
     1381 aagaacggca cgttcagccc gctgccggtc gcccggtccg tccacaacaa cgggccggga
     1441 gcttgggagc tggccgcgcg ctggtcggtg ttcgacgcca actccgggcc aatcagcggg
     1501 ggcaagacgg acatcttcag cctgggcctg aactggtggc tgacaccgct cttcagcgtc
     1561 ggtgccaatt accgctggat caccctggac agggacgggc tgaccggcga gagcgacggg
     1621 ttctcgacgc gcatcctgct gatgctggag taatcggggc ccgacagaga gacgattcgg
     1681 agatcggata acgatgcgga gatcagacaa cgtggcaatc ctaatcaagc tcatgtcgcg
     1741 catagcgatc ctgggcctta tcgcggcgcc cgctgcgcag gcggagacct ggcgcccggt
     1801 cagcgagccc gccgacctcg ccagcctctt cagcgatacc accatgacca cgacgctgcg
     1861 cgacggcgtc accgccaccg cggagtatcg tgcggacggc accggaaccg ttcgggcctg
     1921 gggcgacgct tttgagagaa gatggtcggt cagcgccgat agcgaggttt gcattgaggc
     1981 gggcgatcag actttctgct acacggtgga gaccgacgcc gacgccaccg atcgatatcg
     2041 atcaacaaac ctggagaccg gcgagcggct catcctgatg attgcccgcc agggcggtgc
     2101 ggatccggag ccaacccaag ggacagactc cggagcggcg ggaaagccgt cggccgagga
     2161 ggtcgccgcg aagctcgcca acccaaacac gccgcttgcg agcctgacca ccaagctgca
     2221 gtaccgcacc ttcgacggg
//
LOCUS       DBNHOX010000556         1751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_366484, whole genome shotgun sequence.
ACCESSION   DBNHOX010000556 DBNHOX010000000
VERSION     DBNHOX010000556.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_366484"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="AC16UZ_12220"
     CDS             complement(<1..192)
                     /locus_tag="AC16UZ_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247495.1"
                     /translation="MRADGAGCKRRDVGTAGRGGEDRGMNAIGAGREGTATADREGEA
                     VVLCCPGVGPAIARAGARGD"
     gene            376..>1751
                     /locus_tag="AC16UZ_12225"
     CDS             376..>1751
                     /locus_tag="AC16UZ_12225"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003282385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6247496.1"
                     /translation="MKKAALPENEGERLERLRDYDILDTLPEQDYDDITYLASQICDT
                     PIAVISLVDDDRQWFKSAQGLDVSETPRDVAFCAHTILEPDKLMVVEDASQDDRFADN
                     PLVAGDLGIRFYAGAPLVTASGHALGTLCVIDQKPRGLTDQQRKTLAVLARQVVALLE
                     LRRSVAELKRSSDALHESHQFLALRGQQLNRSRDELAEAVRVLEGQADVIEQDLHRAE
                     VIQRSLLPHEVPRLEDFSVRSLYRPGHTVGGDLYDVVSIADRYLAIVVADACGHGVSA
                     AMLSVLFKQHLRLQDPASGIPYPPAWALERINRSVVANRPAPGVFLTAVYALVDTQTR
                     KLKLGSAGHPPVICLRASGEVESFAHTGPALGLDESSTYEEAEIELGVGDKLLFYTDG
                     LLDICESSPTVEALGQAMVSVADKPNLLEQVLMEVTHGRARPDCDDVTMVLLDAAPGE
                     SVFNESVEG"
BASE COUNT          269 a          596 c          564 g          322 t
ORIGIN      
        1 atctccccgc gctccggccc gggctatggc cgggccgaca ccggggcagc agagcacgac
       61 ggcctcccct tcccgatccg cggtagctgt gccctcgcgg ccggctccga tggcgttcat
      121 gcctcgatct tcgccgccgc ggccggcggt tcccacgtcc cggcgcttgc agcctgcccc
      181 gtcggcgcgc acctcccggc cttctcagcc cgctccgtcg gcccgcacgt cccggccggc
      241 cttcgatccc gggaccattt ccaggcgggg cggcggggtt cgcgccggca ggccgacgcc
      301 catgcggctc gactgagcgc gccagatagc gtttgcggct tcgcctggca gacttaaagg
      361 cgtaatctgt acgtcatgaa gaaggctgcg cttcctgaga acgagggcga acgtcttgag
      421 cggctgcgtg actacgacat tctcgacacg cttcccgagc aggactacga cgacatcacc
      481 tatctcgctt cgcagatctg cgataccccc atagccgtca tcagcctggt ggatgacgat
      541 cgtcagtggt tcaagtcggc ccagggcttg gatgtctccg agacaccgag agacgttgcg
      601 ttctgcgcac acacgattct cgagcccgac aaactcatgg tggtggagga cgccagccag
      661 gatgaccgct ttgcggacaa ccctctggtc gccggcgatc ttggcatacg cttctatgcc
      721 ggcgcccccc tcgtcaccgc atcgggtcac gccctgggca cgttgtgcgt catcgaccag
      781 aaaccccggg gactgaccga tcagcagcgc aaaacgctcg cggtgctcgc tcgccaggtg
      841 gtcgcgctcc tcgaattgcg gcgttccgtc gccgagctta agcgtagctc ggatgccctt
      901 cacgagtctc accagtttct cgcccttcgg gggcagcagc tcaacaggtc gcgggacgag
      961 cttgctgaag ccgtgcgggt gctggagggt caggcggacg tgatcgagca ggatctgcat
     1021 cgggcagaag tgatccagcg ttcgctgctc ccccacgagg tcccccggct cgaggacttc
     1081 agcgtgcgat cgctttatcg cccggggcac acggtgggcg gggatctgta cgacgtggtt
     1141 tcaatcgcgg accgttatct cgccatcgtg gtcgccgacg cctgcggtca cggtgtcagc
     1201 gccgcgatgc tctcggtcct cttcaagcag cacctgcggc tgcaggaccc ggcgtcgggc
     1261 attccttacc cgccggcgtg ggcgctggag cgcatcaacc gttccgtggt cgccaaccgg
     1321 cctgcgcccg gcgtgtttct gaccgccgtc tacgcgcttg tggatactca gacccgaaag
     1381 ctcaagctgg gctccgccgg tcatccgccg gtaatttgtc tgcgcgcgag cggcgaggtc
     1441 gagtcgtttg cccacacggg cccggcgttg gggctcgacg agagttcgac ttacgaggag
     1501 gccgaaatcg agctgggcgt gggcgacaag cttctgttct acaccgacgg gctgctggac
     1561 atctgcgaat cttcccccac ggtggaagcg ctcggccagg ccatggtctc ggtagccgac
     1621 aagccgaacc tcctcgagca ggtgctcatg gaggtgactc acggccgcgc ccgcccggat
     1681 tgcgacgacg tgaccatggt gctgctggat gccgctcccg gcgaaagcgt gttcaacgag
     1741 tctgtggagg g
//
LOCUS       DBNHOX010000557         1695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_302186, whole genome shotgun sequence.
ACCESSION   DBNHOX010000557 DBNHOX010000000
VERSION     DBNHOX010000557.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_302186"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC16UZ_12230"
     CDS             complement(<1..230)
                     /locus_tag="AC16UZ_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247497.1"
                     /translation="MTEIFDEDDEEGLSPHILEQVRRLQARIDRRESLRREHVARSRG
                     LRRRLEDWEEQRALRSSVDYLEMEFAERDGDRR"
     gene            351..1193
                     /locus_tag="AC16UZ_12235"
     CDS             351..1193
                     /locus_tag="AC16UZ_12235"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6247498.1"
                     /translation="METELLRTFLEVNRTRHFARAASNLHLTQAAVSARVRHLEEIVG
                     ERLFTRTRNNIQLTVAGHRLLGHAEAIVGSWNRALLETGAPASTRALVALGCLPSIWE
                     TYLTDWLSRTYEDLSPILLQIELLNTEALVRRLRENSITIGLLYEPPQADDLIAEQVT
                     EVSVRLVSSSQGLSVSDSLPGYVYVDWGTSFAIAHDTRLGISTPPALRVDTPAIAYRF
                     LTEHGGTAYLPSAMVAGALSAGLLFPVAGGKTFTRAAFIARSATASAVDSIKAVTASL
                     RALV"
     gene            complement(1156..>1695)
                     /locus_tag="AC16UZ_12240"
     CDS             complement(1156..>1695)
                     /locus_tag="AC16UZ_12240"
                     /inference="COORDINATES: protein motif:HMM:NF014895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HIR6247499.1"
                     /translation="IRMPPLGVMVEIPAVVDVLPDLAPEIDFACVGSNDLAQYLLAVD
                     RGNPRVVGRYDYLHPGVLGCLDVIARRVEDLGIRLSICGEMAADPVGVVVLLGLGYRA
                     LSMNAYSLPRIRALIRGISIREAQALARSALACRNPGSSRVAIAAALAGLGLDHLLAH
                     GGAFESASTPALAGSPSRP"
BASE COUNT          307 a          583 c          522 g          283 t
ORIGIN      
        1 cggcgatctc cgtcgcgctc ggcgaattcc atttccaggt agtcgacgct cgagcgaagg
       61 gcccgctgct cttcccagtc ttccagccgg cgccgcagtc cccggctgcg cgccacgtgc
      121 tcgcgcctca agctttcccg ccggtcgatg cgggcctgca gccgtcgcac ctgctcgaga
      181 atgtgaggcg ataacccttc ctcgtcgtct tcgtcgaaaa tttcagtcat gtcgctctcg
      241 atgcggggcc gcaacgcccc ggataacgtc ggcgtatata tacgcccggc tatttggcaa
      301 atacatcaaa tatttctgta ctttttgata acagatgttt atcaaaaggc atggaaaccg
      361 agctgctcag aactttcctg gaagtcaatc gcacccgcca cttcgcccgc gccgccagca
      421 acctgcacct gacccaggcg gccgtgagcg cgcgggtgcg ccatctcgag gaaatcgtcg
      481 gcgagcggct gttcacccgc accagaaaca acatccagct gaccgtcgcc gggcaccggc
      541 tgctcggaca tgccgaggcg atcgtcgggt cctggaaccg ggccctcctc gaaaccggcg
      601 ccccggcgag cacgcgggcg ctggtggcgc tgggttgtct gcccagcatc tgggaaacgt
      661 atctgaccga ctggctgagc cgcacctacg aggacctgtc gccgatcttg ttgcagatcg
      721 aactgctcaa caccgaggcg ctcgtcaggc gcctgcggga gaactccatc accatcgggc
      781 ttctctacga accgccccaa gcggacgatc tgattgccga gcaggtcacc gaggtcagcg
      841 taaggctggt ctcgtccagc caggggctca gcgtctcgga ctctctgccc ggttacgttt
      901 acgtcgactg gggaacgtct ttcgccatcg cgcacgatac gcgcctgggg atttcaaccc
      961 cgcccgcgct gcgggtcgac acgccggcta tcgcctaccg ctttctcacc gagcacggcg
     1021 gaacggcgta tctgccgtca gccatggtcg ccggtgccct gtccgcaggg ctgctgtttc
     1081 ccgtcgcagg agggaagacg ttcacgaggg ccgcgttcat cgcgaggagc gcgaccgcca
     1141 gcgcggtcga ttcgatcaag gccgtgacgg cgagcctgcg agcgctggtg tagacgccga
     1201 ctcaaacgcc cccccatggg ccagcagatg atccagcccg agcccggcca gcgccgcggc
     1261 gatggccacg cggctcgacc cgggattacg gcaggccaac gcactgcggg ccaacgcctg
     1321 tgcctcacgg atcgatatgc ctcgaatcag cgccctgata cgcggaaggc tgtatgcgtt
     1381 catgctcagc gcccgatagc cgagcccaag cagcacgacc accccgacgg ggtcggccgc
     1441 catctccccg cagatcgaca agcggatgcc gagatcttcg acgcgccgcg cgatcacgtc
     1501 gaggcagccg agcacgccgg gatgaaggta gtcgtagcgg ccgaccaccc gggggttgcc
     1561 gcggtcgacg gcgagcaggt actgagccag atcgttggaa ccgacacagg cgaagtcgat
     1621 ctccggggcc aggtccggca gcacgtcgac caccgcagga atttcgacca tgacgcccag
     1681 cggaggcatg cggat
//
LOCUS       DBNHOX010000558         5152 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_496129, whole genome shotgun sequence.
ACCESSION   DBNHOX010000558 DBNHOX010000000
VERSION     DBNHOX010000558.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_496129"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /gene="cofH"
                     /locus_tag="AC16UZ_12245"
     CDS             <1..1061
                     /gene="cofH"
                     /locus_tag="AC16UZ_12245"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HIR6247500.1"
                     /translation="NINYTNICYFKCQFCAFSKGKLSENLRGRPYDLSHEEIARRTQE
                     AWSRGATEVCMQGGIHPEYTGETYVGILQAVKEAVPEMHIHAFSPLEVWQGAATLGLD
                     LDTYLERLKAAGLGTLPGTAAEILDDEIRQIICPDKINTEQWLDVMRAAHRVGFKTTA
                     TIMYGHVESAVNWARHLLRIRDLQAETGGFTEFVPLPFVHMEAPMYLKGKARKGPTFR
                     EAILMHAVARLTLHPLIPNIQASWVKMGHAGVQACLQAGCNDLGGTLMNESISRAAGT
                     THGQETSPQRMAEMIKAVGRIPRQRTTLYEDASEERIAVGLAAGELTEIVNTPARKYE
                     RKRSGPLLRPGLIDAVEV"
     gene            1106..2161
                     /locus_tag="AC16UZ_12250"
     CDS             1106..2161
                     /locus_tag="AC16UZ_12250"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR6247501.1"
                     /translation="MAVPWPEAPLFAADVPELQDFLIVEVETASGMVGMGYLQPLAGG
                     LKTLACCVHEMLAPLLVGQEADDVEALWDRMWSATYIQGRMGITVMAMSALDIALWDL
                     VGKRAGEPLYRLWGGVDQHIPTYGSGCYRGLGQDGMIRKAERYAADGFSAIKMQVAHM
                     ATHDQDVENVAAMRAALGADIEIMVDVNQGWTAAEAIEVGSRLDESGIYWLEEPVIAH
                     DFAGYRQVARALKTRVVGGENHFTHHDLQPFFDDPCVPILQPDVMRGGLTELRRIAAM
                     AEPLGISIAPHLFPELMTHLLASIPNGSWLEYMGWHDELWAQPVVPSGGLTRPPDRPG
                     HGLAFRADVLAEYRVAG"
     gene            complement(2158..3384)
                     /locus_tag="AC16UZ_12255"
     CDS             complement(2158..3384)
                     /locus_tag="AC16UZ_12255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6247502.1"
                     /translation="MHDDDKTNDDLGLGRNPTVTLLLAFFQVFLVIMPVAVPFFQSKG
                     LSMQEVFSLQALFALVVLLAEVPSGYVADIFGRKRTLIVGAAFCGLGHTLLLGADGFW
                     TLALFEISLGIGSSLISGADIALLYDTEVALGRGERQQRQVVGRLYSMRTFSEAVAGV
                     VCSVVLLWSMDLAVYVQALIGWLPLVFAFALVEPPGERMERSGHLVNMARICRYLVSH
                     SAVLRLTFLALCIWALTTFYAVWLLQKIWEAQGLGLIHFGYLWGFLSLVAALAGRWAY
                     RAEEMLGTTGVLVFIGLAPAAGYLCLDLFGVVGSVLAGLTFWISRGLGMVILRDALNR
                     RIPGEFRATANSLASFGFRGAFVLTGPLVGYAFDLWGMSVTLSLLALATLVIFAGLVL
                     PLVAAARSQRLEAQAT"
     gene            complement(3814..4776)
                     /locus_tag="AC16UZ_12260"
     CDS             complement(3814..4776)
                     /locus_tag="AC16UZ_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247503.1"
                     /translation="MSLERAYQFARRTNVLVLPAVLMGVEIAVFLAVSPFADSSEILA
                     SMSQAQFVGTLALFSIMPPYFLCMVTLQRLRTEESLAEMADLLEPMSAARVQGCIAGI
                     HPAGWFLVVLGAVFGGYQNEEFIRRVGASGTLNAMDVAFVLANCFVWAAIAFVLSWRL
                     PISLALSRLGAQMKVDVYRLDRLRPLARIATLDILVVAGAMALMPLQSLDAEFRIYNY
                     NYGIIVGVPSAIVLFVLPLWGVRGNIGDRKADRVAVLQAKLDVVDRNDVAAVEALSAH
                     IERLRGLSNWPVDLRLATRIFAYVIIPPLAWVGAALVEILIDRL"
     gene            complement(4784..>5152)
                     /locus_tag="AC16UZ_12265"
     CDS             complement(4784..>5152)
                     /locus_tag="AC16UZ_12265"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6247504.1"
                     /translation="LYDPSGRWTGNIRDEDLQSAGVENAEYLMGPAGSVTVHNCRSVH
                     GSAPNLSERPRPLLLCAYSAADAFPITTLTAGASHSEALIRGEPARWARFDPRPCLMP
                     PDWSRQGGYKSIFADQQKEA"
BASE COUNT         1022 a         1721 c         1644 g          765 t
ORIGIN      
        1 gcaacatcaa ctacaccaac atctgctact tcaagtgcca gttctgcgcg ttctccaagg
       61 gcaagctgtc ggagaatctt cgcgggcgtc cctacgacct gtcccacgag gagattgccc
      121 gccgtaccca ggaagcatgg tcccggggcg ctacggaagt gtgcatgcag ggcggcatac
      181 accccgaata cacgggtgag acttatgtgg gcatcctcca agcggtcaag gaagccgtcc
      241 ctgagatgca catccacgcg ttttccccgc tggaggtctg gcagggcgca gcaacgctgg
      301 ggctggatct ggatacgtac ctcgaacgcc tcaaggcggc cggcctgggt accctgcccg
      361 gaacggcggc ggagatcctc gacgatgaga tccggcagat catctgcccg gacaagatca
      421 acaccgagca gtggctggac gtcatgcgcg cggcccatcg ggtagggttc aagaccacgg
      481 cgaccatcat gtacgggcac gtggagagtg ccgtcaactg ggcccggcac ctgctgcgga
      541 tccgcgatct gcaggcggaa accggcggtt tcacggagtt cgtgccgctc ccgttcgtcc
      601 acatggaagc gcccatgtac ttgaagggca aagcccgaaa gggtccaacc ttccgcgagg
      661 cgatcctgat gcacgccgtg gcgcgcctga ccctgcaccc gctgatccca aacattcagg
      721 cgagctgggt gaagatgggc cacgcgggcg tgcaggcgtg cctgcaggcc ggatgcaacg
      781 atctgggcgg caccctgatg aacgagagca tcagccgggc ggcagggacc acccacggtc
      841 aggaaacgtc accgcagcga atggcggaga tgatcaaggc ggtggggcga atcccgcggc
      901 agcgaacgac cctttacgag gacgccagcg aagagcgcat cgcggtgggc cttgcggcgg
      961 gcgagctcac cgagatcgtc aacacgccgg cgcggaagta cgagcgcaag cgatcgggcc
     1021 ccctcctccg tccgggtctc atcgacgcgg tcgaggtcta atcctgaccc aaccagatcc
     1081 catcgcggac atccggacca cgcggctggc cgtgccctgg ccggaggcac ctctgttcgc
     1141 agccgacgtc ccggaactcc aggactttct gatcgtcgag gtcgagaccg cgtccggcat
     1201 ggtgggaatg ggctacctgc agccgctggc gggcgggctg aaaaccctgg cctgctgcgt
     1261 tcacgagatg cttgcacccc tgctggtcgg ccaggaggcc gacgacgtcg aagcgctgtg
     1321 ggaccgcatg tggagcgcca cctacatcca ggggcggatg ggcatcaccg tgatggccat
     1381 gtcggcgctg gacatcgctc tgtgggacct tgtcggcaag cgcgcgggcg agcccctgta
     1441 cagactatgg ggcggggtcg accagcacat cccgacttac gggagcggtt gctaccgcgg
     1501 cctcggccag gacgggatga tccgcaaggc cgagcgttac gcagccgacg gcttttccgc
     1561 catcaagatg caggtggccc acatggccac gcacgatcag gacgtggaga acgtggccgc
     1621 catgcgcgca gcgcttgggg cggacattga aatcatggtg gacgtcaacc agggctggac
     1681 ggcggcggaa gccatcgagg tcggatcgcg gctggacgaa tcgggcatct actggcttga
     1741 agagccggtg atcgcccacg atttcgcggg ctaccggcag gtcgcccggg ccctgaagac
     1801 acgggtggtc ggtggcgaga accacttcac ccaccacgat cttcagccct tcttcgacga
     1861 tccctgcgtg cccatcctgc agcccgacgt catgcgcggc gggctgacgg agctcaggcg
     1921 gatcgcggcc atggcagagc ccctcggcat cagcatcgcg ccccacctgt tccccgagct
     1981 catgacccac cttctcgcca gcattcccaa cgggagctgg ctcgagtaca tgggctggca
     2041 cgacgagctg tgggcgcagc cggtggtccc gtccggcggg cttacgcgcc cgccggacag
     2101 gcccggacac gggctggcgt tcagagccga cgtcctcgcc gagtaccgcg tcgccggcta
     2161 ggtagcctgg gcttccagcc tctggctgcg ggcggcggca accagcggca gcaccagccc
     2221 ggcgaatatg accagcgtcg ccagcgcgag caggctcagc gtgacgctca tgccccagag
     2281 gtcgaaggcg tagcccacca ggggcccggt caggacgaag gcgccccgga agccgaagct
     2341 cgccagcgag ttggccgtcg cccgaaactc cccgggaatc cgccgattga gcgcatcccg
     2401 caggatcacc atcccgagcc cccgactgat ccagaacgtc agcccggcaa gaacgctgcc
     2461 caccactccg aacaggtcta ggcaaaggta cccggcggcg ggcgccaggc cgatgaagac
     2521 cagcacgccc gtggtgccga gcatctcctc ggcccggtaa gcccaccgtc cggccagagc
     2581 ggcgaccagg ctcaggaacc cccagaggta accgaagtga atcagtccca ggccctgtgc
     2641 ctcccagatc ttctgcagca gccagacggc gtagaacgtc gtcagcgccc agatacacag
     2701 cgccaggaag gtcagccgga gcacggcgct gtggctcacc agatagcgac agatacgggc
     2761 catgttcacc aggtgccccg agcgctccat acgctcgccc gggggctcga ccagagcgaa
     2821 ggcgaacacc aggggtagcc agccgatcag cgcctgaacg taaacggcca ggtccatgga
     2881 ccacagcagc acaacgctgc agacgacgcc ggccactgct tccgagaacg tccgcatcga
     2941 gtacagccga ccgaccacct gccgttgctg acgctcgccg cgccccagcg ccacttccgt
     3001 gtcgtagagc aacgcgatgt cggcgcccga gatcaggctg ctgccgatgc ccagcgatat
     3061 ctcgaacagc gccagcgtcc agaacccgtc cgcgccgagg agcagcgtgt ggcccagacc
     3121 gcagaacgcc gcgccgacga tgagcgtgcg cttgcgaccg aagatgtcgg cgacgtagcc
     3181 ggaagggacc tcggccagga gcaccaccag ggcaaacaga gcctgcagcg agaacacctc
     3241 ctgcatgctc agccccttgc tctgaaagaa cggcacagcc accggcatga tgaccagaaa
     3301 cacctggaag aatgccagca gcagcgtcac cgtcgggttg cggcccaacc cgagatcgtc
     3361 gttcgtcttg tcatcgtcgt gcatcttcat ccctccgacc gttcgccggg acccgccacg
     3421 gacgccggaa tccggcgccc agaaaagaaa aaccccgaag gcttgcggct cttcggggtt
     3481 tttttcggaa ctcgctgctg cgtttcctaa agcatcatcc gctcgagccg tgccctcctc
     3541 gcgcaatacg caccatgtgg tccgcacaca gggactgcgc agacacacgg ccacccgcga
     3601 aggtggtagc tggtttgttt cgatacgcgt tcatgatctc gagcttctct gatccggctc
     3661 acgccgaaaa aacagggcgc gcatgttgcg ccgggcgttc tctggtgtca acggaagtca
     3721 gcaaaaaaag ggggaatctg tgaccccttt cagcaagcca gcctccagac agcttcgagc
     3781 atcggggtcc gatcgctggg gttgggcttg ctgctagagg cgatcgatca gaatctcgac
     3841 cagcgccgcg cccacccatg ccaacggcgg gatgatcacg tacgcgaaga tgcgggtggc
     3901 aaggcgcaga tcgaccggcc agttggaaag accccgcaaa cgttcaatgt gtgcgctcag
     3961 ggcttctacg gcggctacgt cattgcggtc caccacatcc agcttcgcct gcagcacggc
     4021 aacccgatcg gccttgcggt cgccgatgtt cccgcgcacc ccccacaggg gcagaacgaa
     4081 cagcacgatg gcagacggca cacccacgat gatcccgtag ttgtagttat atatccggaa
     4141 ctcggcatcc agggactgca gcggcatcaa cgccatggcg ccggcaacca ccaggatgtc
     4201 cagcgtggcg attcgggcca gcggccgcag ccgatccagc cggtagacgt caaccttcat
     4261 ctgcgcgccc agtcgcgaca gcgccaggct gatcggcagg cgccagctga gaacgaacgc
     4321 gatggcggcc cagacgaaac agttggcaag cacgaaggcc acgtccatgg cgttgagcgt
     4381 gccacttgcg ccgactctgc ggatgaactc ctcattctgg tagccaccga acaccgcccc
     4441 aaggacgacg aggaaccaac ccgcaggatg aatgcccgcg atgcatcctt ggacccgggc
     4501 ggcgctcatc ggctcgagca ggtcggccat ctcggcaagg gattcctccg tcctgagccg
     4561 ctgaagggtg accatgcaga ggaaataggg cggcatgatg ctgaagagtg ccagggtgcc
     4621 aacgaactgt gcctggctca tgctggcgag aatttcgctg gaatcggcaa agggcgagac
     4681 cgcgagaaag acggcgatct ccacgcccat gagcacggcc ggcagcacga gcacgttcgt
     4741 gcgcctggca aattgatagg ctcgctccaa cgacatcggc gcctcaggcc tctttctgct
     4801 gatcggcaaa gatggacttg taacctccct gcctggacca gtcgggcggc atcaggcagg
     4861 gccggggatc gaatcgcgcc cagcgggccg gctcccccct gatcagcgct tcgctgtgag
     4921 aggcacccgc cgtcagcgtg gtgatgggaa acgcgtccgc cgccgagtag gcgcaaagca
     4981 gcagggggcg cggccgctcg ctcaaattgg gcgcagaacc gtgaacggag cggcagttgt
     5041 gaaccgtgac gctacccgcc ggccccatca gatactccgc gttctccacg ccggcgctct
     5101 gcaggtcctc gtcccggata ttgcccgtcc accggccgga ggggtcgtac ag
//
LOCUS       DBNHOX010000559         3137 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_174358, whole genome shotgun sequence.
ACCESSION   DBNHOX010000559 DBNHOX010000000
VERSION     DBNHOX010000559.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_174358"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="AC16UZ_12270"
     CDS             complement(<1..1289)
                     /locus_tag="AC16UZ_12270"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6247505.1"
                     /translation="MDFKESHVRDLLDAAPDATVVADSAGVIVFANAQVGEVLGYAPE
                     ALIGLNVDQLLPERLRAAHGAHRADFMSAPRVRPMGAGLDLYGRRRDGSEFPIEISLS
                     PVSTDQGMLVISAIRDVTERKAFEVELSAAKDTAERATAIKSRFLAAASHDLRQPLQS
                     IAIYLDVLNRVVRQDQDKALEVSAKIDKSLDVMRELLDALLDVSKLDSGSITPEVRDF
                     RLQSLFDELVADCAPEAEGKGLTLSIEPTEAVVRTDPALLQRILANFVGNAVRYTEEG
                     SVAVSAAESGDHLRVEVRDTGIGIPGEAIDTIFEEYFQLDNPVRDRRKGLGLGLSIVK
                     QVASLLDCPLEVASTVGEGSCFSILVPLGLLDEEETVPGQPQTGQLAPGEPVILFVDD
                     DPAIVDATTMMLELAGISVRSALDGNRAIAMLEEGLSP"
     gene            1411..2541
                     /locus_tag="AC16UZ_12275"
     CDS             1411..2541
                     /locus_tag="AC16UZ_12275"
                     /inference="COORDINATES: protein motif:HMM:NF024874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6247506.1"
                     /translation="MTSRQPVRTMVVDPLRRYPSPMLKLKCSVRKAVLIGALAGASPA
                     MAGAPEHHTESGRFTNPYVEQAQGGVFAFLKRRLLGQDTWSSYDAHRDGAVPTAAPRL
                     VAEGEQTNNARVTWVGHATVLIQHAGVNVLTDPILSRIASPVAFAGPGRKSAPGVDAQ
                     GLPPIDVVVISHDHYDHLDEPTIRFLGDRPRYFVPLGLKQWLVDAGIDQSRITELDWW
                     EKQTVEPGGSALTVTATPTQHVSGRTLTDRNKRLWAGWAMEWDDFTVWFGGDTGYNDV
                     QFKAIGDRFDGFDLGIVPIGSYAPRAYMHSYHADPEQAVRIHQDIRARRSMAVHWGTF
                     HLTAEPLLEPPELLRAAVLEAGLPSEEVTAYAVGESRAYFPL"
     gene            2612..2688
                     /locus_tag="AC16UZ_12280"
     tRNA            2612..2688
                     /locus_tag="AC16UZ_12280"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2646..2648,aa:Val,seq:cac)
     gene            complement(2745..>3137)
                     /locus_tag="AC16UZ_12285"
     CDS             complement(2745..>3137)
                     /locus_tag="AC16UZ_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6247507.1"
                     /translation="SIAPYNVAKFGVVALTETLRAELDSEGSAISASVLCPGAINTQI
                     VNSKRNRHSASAHDHQTSDAERAFESRAGRLLAEEGMDPAEVADMVVNAIRGNEFWIV
                     THPQWIDVLKSRVDAMARDRSLARGFGG"
BASE COUNT          567 a         1058 c          964 g          548 t
ORIGIN      
        1 ggagacagcc cctcctccag catggcgatg gccctgttgc cgtcgagcgc cgagcgcacc
       61 gagatcccgg cgagttccag catcatcgtc gtcgcgtcca cgatggcggg gtcatcgtct
      121 acgaagagga tcaccggttc gccaggagcc aactggccgg tctgcggttg gcccgggacc
      181 gtctcctcct cgtccagcag ccccaggggc accagaatgc tgaaacatga gccctcgccc
      241 acggtggaag ccacctccag cgggcagtcg agcaagctgg ctacctgttt cacaatggac
      301 agaccgagac ccagcccttt gcgccggtcg cggaccggat tgtcgagctg gaaatactcc
      361 tcgaagatgg tgtcgatggc ttcccccgga atgccgatac ccgtgtcgcg cacctcgacg
      421 cgcaggtggt ctccactctc ggccgcggag acggcgacgc tgccttcctc cgtataacga
      481 accgcattgc ccacgaagtt ggccaggatc cgctgcagca gcgctggatc cgtgcgaacg
      541 acggcctcgg tgggctcaat ggatagcgtg aggcctttgc cctccgcctc gggagcacag
      601 tctgcgacca gctcgtcgaa cagactctga agccggaaat cccgcacctc tggcgtaatc
      661 gagcccgagt ccagcttgga gacgtccaga agcgcatcga gcagctcacg catcacgtcc
      721 agagacttgt ctatcttggc gctgacttcc aacgccttgt cctggtcctg acgcaccacc
      781 cggttgagca cgtcgagata gatggcgatg gattgcaggg gttgccgcag atcatggctg
      841 gcagctgcca ggaacctgga cttgatcgcc gtggcgcgct ccgcggtgtc cttggcggca
      901 ctcagctcga cctcgaaagc ctttctttcc gtgacgtcgc gtatggcgct gatgaccagc
      961 ataccctggt ccgtgctgac agggctcaag ctgatctcga tgggaaactc cgagccgtcc
     1021 cgacgacgcc cgtagaggtc gagcccggca cccatgggcc ggacccgcgg cgcagacatg
     1081 aagtcggcgc gatgtgcgcc atgggctgca cgcaaccgct cgggcagcag ctggtcgacg
     1141 ttcagaccga tgagggcttc cggcgcgtaa ccgagcacct cgccgacctg cgcattcgca
     1201 aagacgatta cgccagccga atccgccacc acggtcgcgt ccggcgctgc atcgagcaga
     1261 tcacggacgt gactttcctt gaaatccatc gtctccccct caaattacgc tatcagaaac
     1321 gcggcgaggg gagaagcgcc acatggggcc tgaattcaag cctccggggt gcccggagaa
     1381 ggaaccaaat cctggctgga cggtccaaat atgacttccc gtcaaccggt ccgaaccatg
     1441 gtcgtcgatc cgctgaggcg ctaccctagc cccatgctga agctcaaatg ctccgtgcgt
     1501 aaggccgtgc tgatcggcgc gctggccggt gcgagccccg ccatggcggg cgcgccggaa
     1561 caccacaccg aaagcggccg tttcaccaac ccctacgtgg aacaagccca agggggcgtt
     1621 tttgcgttcc ttaaacggcg acttttagga caggacacgt ggtccagcta cgacgcccat
     1681 cgcgatggcg cagtgcccac ggctgcaccc cgcctggtcg ccgagggcga gcagaccaac
     1741 aacgccaggg tgacctgggt cggtcatgcc accgtgctga ttcagcacgc cggcgtcaac
     1801 gtgctcacgg accccatact ttcgcgaatc gcgtcgcccg tcgcgttcgc agggccgggt
     1861 cgtaagtcgg ccccgggcgt agacgcccag ggccttcccc ccatcgacgt ggtggtcatc
     1921 tcccacgatc actacgacca cctcgacgag cctacgatcc gctttctggg cgaccggccc
     1981 cgatacttcg ttcctctggg actgaagcag tggctggtgg atgccggtat cgatcaatcg
     2041 cgaatcaccg agctggactg gtgggagaag cagacggtcg agcccggcgg ctccgcgttg
     2101 acggtgaccg ccacccctac ccagcacgtc tcgggacgga ccctgaccga tcgaaacaag
     2161 cggctgtggg ccggctgggc aatggagtgg gacgacttta ccgtctggtt tggcggggat
     2221 accggataca acgacgttca gttcaaggcc atcggcgatc gtttcgacgg cttcgacctc
     2281 ggcatcgtac ccatcggcag ctacgccccg cgggcgtaca tgcacagcta ccacgccgac
     2341 cctgagcagg cagtgcgcat ccatcaggac atccgcgcga ggcggtccat ggccgtgcac
     2401 tggggcacct tccacctcac tgccgagccg ctgctcgagc cccccgagct gctgcgcgca
     2461 gccgttctgg aggcggggct gcccagcgaa gaggtcaccg cctacgcggt gggcgaatcc
     2521 cgcgcctact tccccctctg aacagttccc agctgcaccc gagcggcgaa gcggtagctt
     2581 cggccgccgt ttttggtagg ctagcttcgc agggcgcgta gctcagttgg ttagagtgct
     2641 gctttcacac ggcagaggtc cgcagttcga atctgcgcgc gcccaccatc tcgcccgggt
     2701 tgccggccga accgtgcgct atgcaggccg gcgccaccgc tggctcaacc tccaaaaccc
     2761 ctggcaaggg accgatctcg ggccatggcg tccactcgcg acttgagcac gtcgatccac
     2821 tgggggtgcg tgacgatcca gaactcgttt ccccggatgg cgttgaccac catgtcggct
     2881 acctccgcag gatccatgcc ctcttccgcc agcagccgtc cggcccggct ctcaaaagct
     2941 cgctccgcat ccgaggtctg gtgatcgtgc gccgaagcgc tatggcggtt tcgtttgctg
     3001 ttgacgatct gggtattgat ggcgccggga cagagcacgg aagctgagat ggcggagcct
     3061 tcggagtcga gctccgcgcg cagggtttct gtcaacgcaa ccacgccgaa cttggcaacg
     3121 ttgtagggcg caatgct
//
LOCUS       DBNHOX010000560         2339 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1779808, whole genome shotgun sequence.
ACCESSION   DBNHOX010000560 DBNHOX010000000
VERSION     DBNHOX010000560.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1779808"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="AC16UZ_12290"
     CDS             complement(<1..184)
                     /locus_tag="AC16UZ_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIR6247508.1"
                     /translation="MSDSEGNQERPPLHCVEEEAGSGSGAASANRMRGIYLLPNLITT
                     GALFAGFYALVAGMNEH"
     gene            complement(269..1288)
                     /gene="ilvC"
                     /locus_tag="AC16UZ_12295"
     CDS             complement(269..1288)
                     /gene="ilvC"
                     /locus_tag="AC16UZ_12295"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR6247509.1"
                     /translation="MQVYYDKDADLSIVQKMNVLVVGYGSQGHAHANNLRDSGVANVT
                     IGLRSDSGSARKATNAGFQVADVADAAKDADLIMILTPDEHQPQIYRDQIAPNVKQGA
                     TLAFAHGFNIHFELIEPRADLDVIMIAPKGPGHTVRSTYVEGGGVPSLIAIFQDASGQ
                     AKNTALSYASANGGGRAGIIETTFQEETETDLFGEQAVLCGGAAALVQAGFETLVEAG
                     YAPEMAYFECLHELKLIVDFFYEGGITNMWYTVSNTAEYGGLTRGPRLVNEETKQEMR
                     RILEEIQTGRFAQEFVAENQAGAPRIKAMRRISQAHEIEQVGERLRAMMPWIQENRLV
                     DKDKN"
     gene            complement(1372..1863)
                     /gene="ilvN"
                     /locus_tag="AC16UZ_12300"
     CDS             complement(1372..1863)
                     /gene="ilvN"
                     /locus_tag="AC16UZ_12300"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR6247510.1"
                     /translation="MRRIIAVLLENEAGALSRVVGLFAQRNYNIDSLTVAPTEDPSLS
                     RLTLTTVGDDRKIEQIIKHLYKVIEVIKVVDLSETDHVERELMLVKVRASGADRSEVK
                     RTCDIFRGQIIDVAPDAYTIQLSGPSEKLDAFIRAIDRALVMEVARTGVSGVSRGDQV
                     LSV"
     gene            complement(1863..>2339)
                     /locus_tag="AC16UZ_12305"
     CDS             complement(1863..>2339)
                     /locus_tag="AC16UZ_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020296510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6247511.1"
                     /translation="GFGLPAAMGVQFAFPDARVVCVTGEGSFMMNMQELSTCLQYGLP
                     IKIVNLNNQALGMVKQWQDMQYGGRHSQSTYSDSLPDFKTLVEAFGHVGFKVDRAEDL
                     EPVMIEAFSPPLRERLVFVDVWVDPHEHVYPMAIKGGAMCDMYLDKITTSQQGDYI"
BASE COUNT          407 a          756 c          707 g          469 t
ORIGIN      
        1 gatgttcatt catccccgcc acgagggcgt agaacccggc aaagagagcg ccggtggtga
       61 ttagattcgg cagcaggtag ataccgcgca tcctgttggc agaggcggcc ccggagcccg
      121 accctgcttc ctcctccacg cagtgaagcg gcgggcgctc ctgattacct tcgctatcgc
      181 tcaaattacc cccttccggg cgggcagcgt ttgccgacaa tcccgtggcg agcgctgcgc
      241 ttggagcacc gcgtcgttgc agcgcaggtc agtttttatc cttgtccacc agacgattct
      301 cctggatcca gggcatcatt gcacgcagcc gctcacccac ctgctcgatc tcgtgggcct
      361 ggctgatacg ccgcatggcc ttgatgcggg gcgctcccgc ctggttttcc gccacgaact
      421 cctgagcgaa tctgcccgtc tggatctcct cgagaatcct gcgcatctcc tgcttcgttt
      481 cctcgttgac caaccgcgga ccacgggtca ggccgccgta ctcggccgtg ttggacacgg
      541 tgtaccacat gttggtgata ccgccttcgt agaagaaatc gacgatgagc ttcagctcgt
      601 ggaggcattc gaaataggcc atctcgggtg cgtagccggc ctccaccagg gtttcgaaac
      661 cggcctggac cagggccgca gcgcctccgc agagcacggc ctgctcgcca aagaggtcgg
      721 tctccgtttc ttcctgaaac gtggtctcga tgattccagc acgcccgccg ccgttggctg
      781 acgcgtagga caacgccgtg ttcttggcct ggccgctcgc gtcttggaaa atcgcgatga
      841 ggctcggcac cccgccgccc tcgacgtaag tggagcgcac cgtgtggccg ggtcctttcg
      901 gcgcgatcat gatgacgtcg agatcggcgc gcggctcgat gagctcgaag tgaatgttga
      961 agccgtgggc gaaagccaga gtggcgcctt gcttgacgtt gggcgcgatc tggtcccggt
     1021 agatctgcgg ctggtgctcg tcgggcgtga gtatcatgat caggtcggcg tccttggcgg
     1081 cgtcggccac gtccgccacc tgaaatcccg cgttggtcgc cttgcgcgca gaccccgaat
     1141 cgctgcgcag gccgatggtc acgttggcaa cccccgagtc gcgcagattg ttggcatgag
     1201 cgtggccctg ggagccgtag cccaccacca ggacgttcat cttctggacg atcgacagat
     1261 ccgcgtcctt gtcgtagtaa acctgcatgt cgcttccccc ttttccagat ccggattgca
     1321 aattcttgtc cgccaccaac atcagcgatc cgcagacgtt catggcccgc cctagacgct
     1381 cagcacctgg tcgccacggc tgacgccgga gacgcctgtg cgggccacct ccatgacgag
     1441 cgcgcggtcg atggcccgaa tgaaggcatc gagcttttcg ctcggccccg agagctgaat
     1501 cgtgtacgca tcgggcgcga cgtcgatgat ctgcccgcgg aatatgtcgc acgtacgctt
     1561 cacctccgag cggtcggcac cagatgcgcg cacctttacg agcatgagct cgcgctccac
     1621 gtggtcagtc tcggaaagat cgaccacctt gatgacctcg atcaccttgt acaggtgctt
     1681 gatgatctgc tcgatcttgc ggtcgtcgcc cacggtggtg agcgtcagcc gcgacaggga
     1741 gggatcttcc gtgggcgcaa cggtgaggct gtcgatgttg taattgcgct gggcgaagag
     1801 ccccaccacc cgggacagcg ctcccgcctc gttttcgagc agcaccgcga tgatgcgccg
     1861 catcagatgt agtctccctg ctggctggtg gtgatcttgt ccaaatacat gtcgcacatg
     1921 gcgccaccct tgatggccat cgggtagacg tgctcgtggg gatcgaccca gacatccacg
     1981 aacacgaggc gctcacgcag cggcggcgag aaagcttcga tcatgaccgg ctccagatcc
     2041 tcggcccggt ccaccttgaa gcccacatgg ccgaacgctt ccaccagggt cttgaagtcc
     2101 ggcagcgagt cactgtaggt gctctgagag tgacgtccgc cgtactgcat gtcctgccac
     2161 tgcttgacca ttcccagggc ctggttgttg aggttgacga tcttgatcgg cagaccgtac
     2221 tgaaggcagg tggacagctc ctgcatgttc atcatgaagc ttccctcgcc ggtgacgcag
     2281 accacccggg catccggaaa ggcaaactgc acgcccatgg cagcgggcag cccgaagcc
//
LOCUS       DBNHOX010000561         2185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1265499, whole genome shotgun sequence.
ACCESSION   DBNHOX010000561 DBNHOX010000000
VERSION     DBNHOX010000561.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1265499"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="AC16UZ_12310"
     CDS             complement(<1..700)
                     /locus_tag="AC16UZ_12310"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6247512.1"
                     /translation="MATISQRANLMADASALSEDPKEQTWQQTKSALTRTAILEAAVL
                     CFYELGYASTTTENIANAAGVSRGAMLHHFPTRFDLIRAAVEHLNQKRLDWYAAEEEE
                     IQRGAEHTRIEEGIDTYWKQLNTPEFIVFHELNVAARTDPELAEVVIPALREFRQKSE
                     EASRQVFPDLALSEAWERTNFLSRFLLEGMAAAKMIEGPEVPDQQMLEWLKRELRRSY
                     QDVLNTVRRPGSPTD"
     gene            757..1572
                     /locus_tag="AC16UZ_12315"
     CDS             757..1572
                     /locus_tag="AC16UZ_12315"
                     /inference="COORDINATES: protein motif:HMM:NF018038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR6247513.1"
                     /translation="MRSILLVTKGHPFEREPFFQIFDVMDGVDWTHVEHPAAEALFNV
                     REAEAYDAFVMYDMPGIRFHPDRAPDFAEPREDFRRQFLELVDSGHGFVFLHHAVAGW
                     PAWDEFAEIVGGRFLYLPAELRGRSCPDSGYRHGVEHTVSVVAKHPVTEGIPGSFSIT
                     DELYLYEVFEDSVEPLLRSDFSFTRDNFYSAARVVRDQKMYDNEGWEHEPGSNLVGWT
                     RNRGNSRIVYLQCGDDPVAYANPHFQKLIRNAIFWVSEASARGRAGSGGRTGA"
     gene            1681..>2185
                     /locus_tag="AC16UZ_12320"
     CDS             1681..>2185
                     /locus_tag="AC16UZ_12320"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6247514.1"
                     /translation="MTVYRKIERFADPQTSRTPPQKEPDPDLGYDIIPKERYTSPEFM
                     QLEWDRMWTKVWLMGAWIGDLQNPGDYVVTEIGKESIVITRDENGDYNAFYNVCSHRG
                     NQVAYGKGGNTKTFRCSYHLWEYNLRGEIVNVPDQETFPQGVPCEKLAIVKLPCAAWG
                     AWVWFSLN"
BASE COUNT          385 a          657 c          714 g          429 t
ORIGIN      
        1 cgtcggtggg cgagccgggt cggcggacgg tgttcagcac gtcctggtag ctgcgcctga
       61 gctcgcgttt gagccattcg agcatctgct gatcgggaac ctccgggccc tcgatcatct
      121 tcgcggccgc catgccctcc agcagaaacc gggagagaaa gttcgtccgt tcccaggctt
      181 cggagagcgc caggtcgggg aagacctggc gggaggcctc ctctgatttc tgccggaact
      241 cgcgaagcgc cggtatcacc acctcggcaa gctcgggatc cgtgcgggca gcgacgttca
      301 gctcgtgaaa gacgatgaac tcgggcgtgt tcagctgttt ccagtaggta tcgatgcctt
      361 cctcgatcct ggtgtgctcg gcgccgcgct ggatctcctc ttcctccgcc gcgtaccagt
      421 ccaggcgctt ctggttgagg tgctcgaccg cggctcggat cagatcgaag cgcgtgggga
      481 agtgatggag catcgctccc cgcgacacgc cggccgcgtt ggcaatgttc tccgtcgtgg
      541 tgctggcgta tccgagctcg tagaagcaga gaacggcggc ctcaagaatc gcggtgcgcg
      601 tcagcgcgct cttggtctgc tgccaggtct gttcttttgg gtcctcgctc agcgcgctgg
      661 cgtctgccat gaggttggcc cgctgtgata tcgttgccat gttatgcgcc gtgcccaagc
      721 ggagcaaccg cgcggggcag gatgcatggg gggagcatga gaagcattct gctggttacg
      781 aagggtcatc ccttcgagag ggaaccgttc tttcagatct tcgatgtcat ggacggtgtc
      841 gactggaccc acgtggagca tcccgccgca gaggctctgt tcaacgtccg cgaggccgaa
      901 gcctacgacg ccttcgtgat gtacgacatg cccggcatcc gttttcatcc cgaccgggcg
      961 cccgatttcg cggagccccg agaggatttc cgccgtcagt ttctggagct cgtcgacagc
     1021 ggtcacgggt tcgtttttct gcaccacgcg gtggccggat ggccggcatg ggacgagttt
     1081 gccgagatcg tcggcgggcg gtttctctat ctgccggcag agctgcgggg ccggtcctgt
     1141 ccggattccg gataccgtca cggcgtcgag cacacggtca gcgtggtggc gaaacatccg
     1201 gtgaccgagg gcattccggg ctcctttagc attacggacg agctgtatct ctacgaggtg
     1261 ttcgaggaca gcgtggagcc ccttctgcga agcgacttct cgttcacccg ggacaacttc
     1321 tactccgccg cacgggtcgt tcgcgaccag aagatgtacg acaacgaagg ctgggagcac
     1381 gagccgggca gcaacctggt gggctggacg cgcaatcgcg gcaacagccg catcgtctac
     1441 ctgcagtgcg gagacgaccc ggtggcctac gccaaccccc attttcagaa gctgatccgc
     1501 aacgcgatct tctgggtttc ggaggcgtcg gctcggggcc gcgccgggag cggcggccgc
     1561 acgggtgcct gaacgggcat gcgggggtgc aaaccggcgg cggtggtggt acaaatcgcc
     1621 tgttaaaacc tgcacagttg actgttttca gctggcagcc aagagaggag gattaacggc
     1681 atgacggttt atcgcaagat cgagcggttc gcagacccgc agacttcgag gacgccccct
     1741 cagaaggaac ctgatcctga tctgggctac gacattattc cgaaagagcg gtacacgtcc
     1801 cccgagttca tgcagctcga gtgggatcgg atgtggacca aggtctggct catgggtgcc
     1861 tggatcggcg atctgcagaa tccgggcgac tacgtcgtca ccgagatcgg caaggagagc
     1921 atcgtcatca cccgggacga gaacggcgat tacaacgcgt tctacaacgt ctgctctcat
     1981 cgcggtaacc aggtggcgta cggtaagggc ggcaacacca agacgttcag gtgctcctac
     2041 cacctctggg agtacaacct gcggggcgag atcgtcaacg ttcccgacca ggagaccttc
     2101 cctcagggcg ttccctgcga gaagctcgcc atcgtcaagc tcccctgcgc tgcgtggggt
     2161 gcctgggtat ggttcagcct caacc
//
LOCUS       DBNHOX010000562         9488 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_109966, whole genome shotgun sequence.
ACCESSION   DBNHOX010000562 DBNHOX010000000
VERSION     DBNHOX010000562.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9488)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9488)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9488
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_109966"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="AC16UZ_12325"
     CDS             complement(<1..818)
                     /locus_tag="AC16UZ_12325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR6247515.1"
                     /translation="MSEGGQAGELQGRRPGIWELAFPSILSNLSFTLVGLVQTKFVGE
                     LGAQALAAVGAGQRVFFAMQAILMAVSAGTTALVARAWGAGDYVEASRVTMASLVLAG
                     AFSVVVAVIGLFFAPVIAGVFGLDPETLGMASENIRWLCVFNVAFAVNFILGAALRAS
                     GDAWSPLWISGAVNVVNVPLLYVFIFGEFGFPRMGVAGAAIAAGIAFSIGGAVLLGLW
                     VKQKFRVKHVASGWWRKARLRQLLDIGYPAAVEQGVFQLGFFIFLMLIGNFYGT"
     gene            920..1720
                     /locus_tag="AC16UZ_12330"
     CDS             920..1720
                     /locus_tag="AC16UZ_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6247516.1"
                     /translation="MTEYTDIGVTRDGHVTTVEIQRPPNNFFDHSLIRQIADAFNAAD
                     EDPNCRAIVLAAQGRHFCAGANFGSGKDDDSGSEDFTEEGFRNTTGILYREAARLFGS
                     KKPVIGAIQGAAIGGGLGLSLVPDFRVACPEARFGANFVKLGIHQGFGISVSLPRIIG
                     QQAASMLLLTGRRIGGEEAFKLGLVDVLTDAESLRDEAMKLAQEIAENAPLAVLSVRG
                     TMRAGLAEAVIEATEHELKEQQWLRATEDAQEGIRSVAARRPGNFQGR"
     gene            1722..2594
                     /locus_tag="AC16UZ_12335"
     CDS             1722..2594
                     /locus_tag="AC16UZ_12335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6247517.1"
                     /translation="MKLGKLGVWYFLDGLPADAAAAAAQRIEELGYSALWIPEATGRN
                     PMVHASWLLANTKQLIVATGIANIYHREPGVTVSAQKALAEQSGDRFLLGIGVSHKPF
                     VEGVRGLTYGPPVASMRDYLDKMAAAPYTSVPPAEDPPTVIAALGPKMLALAAEKTAG
                     AHPYFVSPDHTAMASEVLGPDKWLCVEQKVILETDPSRARELARPAAQMYLALPNYRN
                     NWLRMGFTEADLGNGGSDRFIDATFAWGSAAQVEARIREHFDAGATHVCIQPVNPNGQ
                     FGDLHWEALEALAP"
     gene            2591..3274
                     /locus_tag="AC16UZ_12340"
     CDS             2591..3274
                     /locus_tag="AC16UZ_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6247518.1"
                     /translation="MSSDYTGNVVVVTGAAGALGRAVVEHFAASGATVAQLDVVAIDN
                     AHFSDTCDLTDPDSCRQAVTRIIETLGRIDVLANIAGGFTMGEAVHETSEETWDFMMG
                     LNARSVLNMARAVVPIMLQQGAGKIVNVGAGAGLAGVANMGAYSASKSVVIRLTEAMS
                     AELKERRINVNCVLPSIIDTERNRADMPDADFHRWVTPAAMAGVIGFLASPAADPIHG
                     AAVPVSGLS"
     gene            complement(3309..5051)
                     /locus_tag="AC16UZ_12345"
     CDS             complement(3309..5051)
                     /locus_tag="AC16UZ_12345"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6247519.1"
                     /translation="MVNRRRFVEAGALTAAGLSLWGCGADTRPRSAGAGDGQVAGPGR
                     AVVFRSGVVLPVDAGFSEQRAIAIRGNRILAVGDDDDVLAAAGKDPQVVDLDGRVLLP
                     GFIEPHMHFALMAGLGHWPDIGPFKHESTEDALAALRLIARETPADEWIVARQFDPSL
                     QTGPEMLTTRELDTVSTDRPVFVLNASGHLAYGNSRLLEFAGIDRDTPDPPGGEYFRD
                     PDGRPNGAMSQVAYQPVMMRNEALIAVLGSGFVEAGLRVGEEAAALGITTLCDQATGG
                     IAGMGDIDFYRQMFASSRMSARIRASLYSETAAEWDDNGVAFGDGDALMRIVGWKIVT
                     DGSNQGFTGRQREPYLDSDNRGMYYVAPEALNEMVLSRGRRGWPLVLHGNGDAAIDSI
                     LDAVQAAADAGVDVGKLRCRIEHASILHDGQIQRIRELGMGPSFLINHVHYWGHAMRD
                     KVFGPEKVQLLDRCRSVEDAGITWTMHSDAPVSPLGTLHKIRVAVARDLWKEPDTTLA
                     PHERVSVEAAIRAVTINAAWQCHSETEVGSLAEGKLADLVVLEDDPRKVEPKAISEIG
                     VSQTWMDGNQVFGA"
     gene            5326..6423
                     /locus_tag="AC16UZ_12350"
     CDS             5326..6423
                     /locus_tag="AC16UZ_12350"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024151.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6247520.1"
                     /translation="MTSYDPFLRGPHPVGVRTFHRTDKTRQRTLPVEVWYPADDEHAG
                     QDLDPDTQDHFQPMSLSPPVPQAAVRDAAPAEGEPRPLLIFSHGFGGTGRQTTHLCTH
                     WASHGYVVAAMDHVGNTTEDVMAAAAQPVDPGTLLERLKSFAADRPVDVSFVIDRMVA
                     GDAGLTVDPDRVGVSGHSFGGWTTLASAGMDPRIRAALPLAPAGGQSSQWQSSIADRL
                     ADALAMSWEREVPTLFLVAEHDVVLPLDSMHDLIERTPHPRRAVNLLNADHFHFCDRV
                     EQTHDMVRMMGQMIFGTRSEADQEAPSALAAMKPSSELCPGDHAYALLQGLGLAHMDA
                     HVRELDRAHALLAGDLASLMAERGVAVKVMA"
     gene            complement(6476..6955)
                     /locus_tag="AC16UZ_12355"
     CDS             complement(6476..6955)
                     /locus_tag="AC16UZ_12355"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6247521.1"
                     /translation="MSDTETNMNNALEILRQRLDQPNPSSDWFEITQDRVNDYADVSM
                     DHQWIHVDVERAKQGPFGAPIAHGNLTIAVMGHLPRSETASQPPLEGHKLSINYGFDR
                     IRFPAPVRVGDRIRATSTPRRAEIKGGMIETMNEIVVEIEGQEKPALVAESLGRLVF"
     gene            complement(7064..8185)
                     /locus_tag="AC16UZ_12360"
     CDS             complement(7064..8185)
                     /locus_tag="AC16UZ_12360"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR6247522.1"
                     /translation="MNLKTRVCEVLEIEHPIVLAGMGGASCPALAAAVSNAGGLGVLG
                     AAGCGPELLRRWIRETRELTDRPFGVDTLLPASVRRANYDETEGPTPMDLLPETQAYA
                     AEFMEREGLEPVASESPRPPRTSDEPAVFTKDFFEAQMRVVIEERVPVYASGLGNPGP
                     WMDELRANGTKVMAVVGAVRHAIQVKSSGLDIIVAQGHDGGGHNSPIGTMALIPQVVD
                     AVGDTPVLGAGGIVDGRGIAAAMMLGAEGVWIGSAFLASQEANIFDFQKQAIVDATEE
                     GTSVSRSVTGKPARIIRSKWTDYWARGERDPLPMPFQGLVSQPVLAAANRDGRGDINP
                     GFAGQGIGMIKAVRPAREIFDELVYGAEQALARTSNLLR"
     gene            complement(8185..8670)
                     /locus_tag="AC16UZ_12365"
     CDS             complement(8185..8670)
                     /locus_tag="AC16UZ_12365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR6247523.1"
                     /translation="MSEIDQRVFRDTMGQFCTGVVIATGCREGEPAGFAAQSFSSVSL
                     DPPLVVLCPAKTSTSWPKLRDSGSFCINILGEDQKAVCDVFAQSGVEKFASLDWSPGA
                     TGSPVLADVIAYVECDLHAEHDAGDHTLAVGLVREVSIVNPDKPPLLFFRGGYGQFEG
                     L"
     gene            8793..>9488
                     /locus_tag="AC16UZ_12370"
     CDS             8793..>9488
                     /locus_tag="AC16UZ_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6247524.1"
                     /translation="MTDLNEFRKEVRNWLEENCPPSMRTSTPEGETVWGGRREKFANP
                     DSRIWLDRMAERGWTAPTWPREYGGGGLTADEDKVLQQELRSINARPALQSFGIWMLG
                     PALLEFASEEQKLEHIPQIVRGEIRWCQGYSEPGYGSDLAGLQTKAEDQGDHFLVNGS
                     KIWTSYADVADWIFCLVRTDNDAPKHEGISFLLFDMDSEGVTTSPIELISGASPFCQT
                     FFDNVRVPKANLVG"
BASE COUNT         1696 a         3237 c         3040 g         1515 t
ORIGIN      
        1 tcggtaccgt agaagtttcc gatcagcatc aggaagatga agaagccgag ctggaacacc
       61 ccctgctcga ccgctgccgg gtagccgatg tcgagaagct ggcgcaaccg cgccttgcgc
      121 caccagccgc tcgccacgtg cttgacccgg aacttctgtt tgacccagag cccgagaagg
      181 acggccccgc cgatggagaa cgcgatgccg gccgcaatgg ctgctccggc aacccccatg
      241 cggggaaaac cgaactcgcc gaagatgaac acgtagagca gcggaacgtt gacgacgttg
      301 accgcgccgc tgatccaaag aggcgaccag gcatctccag atgcgcgcag cgcggcgccc
      361 aggatgaagt tgacggcgaa ggcgacgttg aaaacacaca gccagcggat gttctctgag
      421 gccatgccaa gcgtttccgg atcgagcccg aagacgccgg caatgacagg ggcgaaaaac
      481 agaccgatca ccgcgaccac gaccgagaag gcgccggcga gaaccagcga ggccatggtg
      541 acgcggctcg cctcgacgta gtccccggcg ccccaggctc tggccactag agccgtggtg
      601 cccgcgctga ccgccatcag aatggcctgc atggcaaaga agacacgctg ccctgcgccc
      661 acggccgcga gcgcctgggc acccagctcg ccgacgaact tggtctgcac aagaccaacg
      721 agggtgaatg acaggttgct caggatggac ggaaacgcca gttcccagat gcccggtcgc
      781 cgtccctgaa gttctcctgc ctgaccgcct tcggacatct gcctccttct cgaacgcacc
      841 aggttgctta tggaatgccc gccgcgctgg ctgctaatat cctccgctcg ctcgaattca
      901 gcaaacgggg ataaggatct tgactgaata caccgacatc ggcgtgaccc gggacggcca
      961 cgtcactacc gtggagatac agcgtccgcc aaacaatttc ttcgatcaca gcctgatcag
     1021 gcagatcgcc gacgcgttca acgcagcaga cgaggatccg aactgcaggg ccatcgttct
     1081 ggcagcacag ggccggcatt tctgcgcggg ggccaatttc ggcagcggaa aggacgacga
     1141 ctccggaagc gaggatttta cagaggaggg ctttcgcaac accacgggca ttctctaccg
     1201 cgaggccgcg cggctgttcg gcagcaagaa accggtaatc ggcgcgatcc aaggcgccgc
     1261 catcggcgga ggcctgggtc tgtccctggt cccggatttt cgcgtcgcct gtccggaggc
     1321 gcgattcgga gccaacttcg tcaagctcgg cattcaccag ggcttcggca tctccgtcag
     1381 cctgccccgc atcatcggtc agcaggctgc aagcatgctg ctgctgacag gccggcgcat
     1441 cggcggcgaa gaggcgttca agctcggcct ggtggacgta ctgaccgacg ccgagagcct
     1501 gcgcgacgag gcgatgaagc tggcgcagga aatcgccgag aacgccccgc tggccgtcct
     1561 gtcagtcagg ggcaccatgc gcgccgggct cgctgaggcc gttatcgagg ccaccgagca
     1621 cgagctcaag gagcagcaat ggctacgcgc caccgaggac gcccaggaag gcatccgctc
     1681 cgtggccgcg cgtcggcccg gcaacttcca gggccgttga ggtgaagctc ggcaagctcg
     1741 gcgtctggta ctttctcgac ggcctgccgg cggatgccgc ggccgccgcc gctcagcgca
     1801 tcgaggaact gggttacagc gctctgtgga taccggaggc gacggggcgc aaccccatgg
     1861 ttcacgcctc gtggctgctg gccaacacca agcagctgat cgtggccacg ggaatcgcca
     1921 acatctacca ccgggaaccc ggcgtcacgg tctcggcgca aaaggccctg gccgagcagt
     1981 cgggcgaccg gttcctgctc ggaattggtg tttctcacaa gcccttcgtg gagggcgtgc
     2041 gcggcctgac ttacggcccg cccgtggcga gcatgcggga ctatctggac aagatggccg
     2101 ctgcgcccta cacgagcgtt ccgccggccg aagacccgcc cacggtgatc gccgcgctgg
     2161 gcccgaagat gctggccctg gccgccgaga agactgcagg cgcccacccc tacttcgttt
     2221 cgcccgacca caccgccatg gccagcgaag tcctcggacc ggacaagtgg ctgtgcgtag
     2281 agcagaaagt cattctggag acggatcctt cccgggcccg ggagctcgcc cgcccggcgg
     2341 cgcagatgta cctggccctg cccaactacc gaaacaactg gttgcgcatg ggcttcaccg
     2401 aagccgacct cggcaacggc ggttccgatc ggttcatcga cgccaccttt gcctggggca
     2461 gcgcggccca ggttgaagcc cgcattcgcg agcattttga tgccggcgcg acccacgtct
     2521 gcatccagcc cgtcaatccc aacggtcagt ttggcgatct acactgggaa gccctggagg
     2581 cgctcgcgcc atgagcagcg actatacagg caacgtagtc gtcgtcacgg gcgcggccgg
     2641 cgccctgggc cgtgccgtgg tcgagcactt cgccgcaagc ggcgccaccg tggcgcagct
     2701 agacgtggtg gccatcgaca acgcccactt ctccgacacc tgtgatctga ccgacccgga
     2761 cagctgcagg caggcggtca cccggataat cgagacgctg gggcgcatcg acgtgctggc
     2821 caacatcgcc ggcggcttca ccatgggaga ggccgttcac gaaacctccg aggagacctg
     2881 ggatttcatg atgggcctga acgcgcgctc ggtgctgaac atggcccggg ccgtggtccc
     2941 catcatgctt cagcaggggg ccggcaagat cgtcaacgtc ggcgccggcg cagggctcgc
     3001 cggagtcgca aacatgggtg cctacagcgc gtctaagagc gtggtcatcc gattgacgga
     3061 agccatgtcc gcggagctca aggagcgacg catcaacgtc aactgcgtgc tgccgtccat
     3121 catcgatacc gagcgcaacc gggccgacat gcccgacgcc gatttccacc gttgggtgac
     3181 gcccgcggcc atggccgggg tcatcggctt tctggcgtca cctgcagcgg accccattca
     3241 cggcgccgcc gtgcccgtca gcgggctgag ctgaggggcg cgggccgacc ggcccgcgac
     3301 gctgcggatc aggctccaaa tacctggttc ccgtccatcc acgtctggct caccccgatc
     3361 tcggagatgg ccttgggctc gaccttcctc gggtcgtcct ccagcacgac cagatcggcc
     3421 agcttgcctt ccgctaggct gccgacctcg gtctccgagt gacactgcca tgccgcgttg
     3481 atggtgacgg cccttatcgc cgcctccacg gaaacgcgct cgtggggcgc aagtgtggtg
     3541 tcgggctcct tccagaggtc gcgggccacc gcaacccgga tcttgtgcag ggtgccaagc
     3601 ggtgacaccg gcgcgtcgct gtgcatcgtc caagtgatgc cggcatcttc gacgctccgg
     3661 cagcgatcga gcagctgcac tttctccggg ccgaacacct tgtcgcgcat ggcgtgaccc
     3721 caatagtgca cgtggttgat gagaaagctg gggcccatgc ccagctcgcg aatgcgctgg
     3781 atctgcccat cgtggaggat cgacgcgtgc tcgatgcggc agcgcagctt tcccacgtcc
     3841 acgcccgcat ccgccgccgc ctgcaccgcg tccagaatgc tgtcgatcgc ggcgtcgccg
     3901 ttgccgtgca gcaccagcgg ccagcctcga cggccacggc tgagcaccat ttcattcagc
     3961 gcttcgggcg cgacgtagta catgccccga ttgtccgagt ctagatacgg ctcacgctgc
     4021 cggcccgtga aaccctggtt ggacccatcg gtcacgatct tccagcccac aatgcgcatc
     4081 agggcgtccc cgtcaccgaa ggcaacgccg ttgtcgtccc actccgcggc cgtctcggaa
     4141 tagagcgagg cgcgaatccg cgcgctcatc cggctcgagg caaacatctg ccggtagaag
     4201 tcgatatcgc ccatgccggc aatgcccccg gtggcctggt cacagagggt ggtgatcccg
     4261 agcgcggccg cctcttcgcc gacccgcaac ccggcctcaa cgaacccgga gccaagaacc
     4321 gcgatgagcg cctcgttccg catcatcacc ggctggtagg cgacctggga catggcgccg
     4381 ttgggccgcc catcggggtc acggaaatac tccccgccgg gcggatccgg cgtgtccctg
     4441 tcgatgccag cgaactccag caggcgggag ttaccgtagg cgagatggcc cgaggcgttc
     4501 agcacaaaga cgggccggtc tgttgacacg gtatctagct cccgggtcgt cagcatctcg
     4561 ggacccgtct gaaggctggg atcgaactga cgggcaacga tccactcatc agccggcgtc
     4621 tccctggcaa tgaggcgcag agcggcgagc gcgtcctcgg tgctttcgtg tttgaacggg
     4681 ccgatgtcag gccagtggcc cagcccggcc atcagcgcga agtgcatgtg gggttcgatg
     4741 aatcccggca ggaggacccg gccatcgaga tcgaccacct gggggtcttt ccccgccgcc
     4801 gccagcacgt cgtcgtcatc cccgacggca aggatgcggt ttccgcggat ggcaatcgcc
     4861 ctctgctcgg agaatcccgc atccaccggc aacaccacgc cgctgcggaa aacgacggcg
     4921 cggccgggac ccgcaacctg cccgtcgccg gccccggcag acctcggacg ggtatcagca
     4981 ccgcaacccc acagcgacaa gccggcagcc gtcagggcgc cggcctctac gaatcgtctt
     5041 ctgttaacca tcccggcggg cctccaaaat ctgttcttcc ctcgacttcc ggtgagtcct
     5101 gccgccaacg ggccacggtc tcctttgctg cgtcagcgta cacggcgagc cagccgaaac
     5161 gaagcaaccc gatacatgca aggctcatgc tggcggcaat ggaatcctct cggtatcctt
     5221 cgcggcgcgt cgagatctcg agagtcgagc cagcctcgat tcgggcagca ccacgctgcc
     5281 ggattgcgag cccgacgctc aaccgttctg acacagaggt agacgatgac atcgtatgat
     5341 ccgttcctgc gtggcccgca tccggtcggc gtgcgcacct ttcaccggac cgacaaaaca
     5401 aggcaacgaa cgttgcccgt ggaagtgtgg tacccggccg acgacgagca cgccgggcag
     5461 gatctcgacc cggataccca ggatcacttc cagcccatga gcctgtcgcc accggtaccc
     5521 caggcagccg ttcgcgacgc ggcaccggcc gagggtgagc cccggcccct gctgatcttc
     5581 tctcatggct tcggcgggac cggccgccag acgacacacc tgtgcaccca ttgggccagt
     5641 cacggctacg tggtcgccgc catggaccac gtcggcaaca ccacggagga cgtgatggcg
     5701 gcggccgccc agccagtgga tccagggacc ctgctcgagc gcctgaaatc gtttgccgca
     5761 gaccggccag tggacgtcag cttcgtgatc gaccggatgg tggccggcga tgccggcctg
     5821 accgtcgatc ccgaccgcgt tggcgtctcc gggcacagct tcggcggctg gaccaccctg
     5881 gcatcggccg gaatggatcc acgcatccgc gccgccttgc ccctggcacc ggccgggggc
     5941 caatcgagcc agtggcagtc gagcatcgcc gaccgcctgg cagacgcgct cgccatgagc
     6001 tgggagcgcg aggtacccac gctgtttctg gtcgccgagc acgacgtggt cctgccgctg
     6061 gacagcatgc acgacctcat cgagcgaacg ccgcacccgc gccgcgccgt gaatctactg
     6121 aacgccgatc acttccactt ctgcgatcgg gtggagcaga cccacgacat ggtgaggatg
     6181 atgggacaga tgatcttcgg cacgcgcagc gaagcggacc aggaagcgcc ctccgcgctg
     6241 gcggccatga agccgagcag cgagctgtgc cctggcgatc acgcctacgc gctgcttcag
     6301 gggctgggcc tggcccatat ggacgcccac gtgagggagc tcgaccgggc ccacgcgctg
     6361 ctggcgggcg acctggcgtc gctgatggcc gaacggggcg tggcggtcaa ggtcatggcc
     6421 tgagcagcgc acgccggcac cccccgttcc cccgtccggg tgacggcccg gcagatcaga
     6481 agacgaggcg acccaggctt tcggccacca gcgccggctt ttcctgaccc tcgatctcga
     6541 ccacgatctc gttcatggtc tcgatcatgc cgcccttgat ctcggcgcgg cgcggcgtgc
     6601 tggtggcgcg gatcctgtcg cccacccgca cgggcgcggg aaaacggata cggtcgaagc
     6661 cgtagttgat gctgagcttg tggccctcca gcggtggctg ggaggccgtc tcagatcgcg
     6721 gcaggtggcc catcacggcg atggtcaggt tgccgtgggc gatgggcgca ccgaacggcc
     6781 cctgcttggc ccgctcgacg tcgacgtgga tccactggtg atccatgctg acgtcggcat
     6841 agtcgttgac ccgatcctga gtgatctcga accagtctga ggaggggtta ggctggtcga
     6901 gacgctgtcg cagaatctcc agggcgttgt tcatgttggt ttcggtgtcg gacatgatct
     6961 cgttctctgg taatcgtcaa aaatcaaacg ggcggggccc accggctggc atcgtcgttc
     7021 gcatcgaggg agccgatgag gtccacgatc gggccggcgc cgctcagcga agcaggttcg
     7081 aagttcgagc gagcgcctgc tcggccccgt aaaccagctc gtcgaatatt tcccgggccg
     7141 ggcggacggc ctttatcatg ccgatgccct ggccggcgaa acccgggttg atgtcgcccc
     7201 ggccatcgcg gttcgccgcg gccagcaccg gctgggagac caagccctgg aagggcatcg
     7261 gcaggggatc ccgctcgccc cgcgcccagt aatcggtcca tttgctgcga atgatccgtg
     7321 ccggcttacc cgtcacggac cgggacacgg aggttccctc ctcggtggca tcgacgatgg
     7381 cctgcttctg aaaatcgaag atgttggctt cctgggacgc gagaaacgcg gagccgatcc
     7441 agaccccctc ggctccgagc atcatggctg cagcaatgcc tcggccgtcg acgatgccac
     7501 cggcgcccag caccggcgta tcgcccaccg cgtccaccac ctggggtatc agcgccatgg
     7561 tgccgatggg cgaattgtga ccgccgccgt cgtgaccctg ggccacgatg atgtcgagcc
     7621 cggaggactt cacctgaatc gcgtgccgga cggcgcccac cacggccatc accttggtgc
     7681 cgttggcgcg cagctcgtcc atccacggtc cggggttgcc cagaccggac gcgtaaacgg
     7741 ggacccgctc ctcgatgacc acgcgcatct gcgcctcgaa gaaatctttc gtgaacacgg
     7801 cgggctcgtc ggacgttcgc ggcggccgcg gcgactcgct cgccaccggc tccaggcctt
     7861 cgcgttccat gaactcagcc gcgtaggcct gggtctcagg cagcagatcc atgggcgtcg
     7921 gcccctcggt ctcgtcgtag ttggcgcgcc gaaccgaggc aggcagcagc gtgtcgacac
     7981 cgaagggtct gtcggtgagc tcgcgcgtct cgcggatcca gcgccgcagg agctccggac
     8041 cacatcccgc ggctcccagc acaccgagcc cgccggcgtt ggataccgcc gcggccaggg
     8101 ccgggcagct cgccccgccc attcctgcga gaacgatggg gtgctcgatt tctagcactt
     8161 cgcagacgcg cgtcttcaga ttcatcacag tccctcgaat tgaccatagc cgccgcggaa
     8221 gaacaggagc ggcggcttgt cggggttgac gatgctgacc tcacgcacca gcccgacggc
     8281 aagcgtatgg tcgcccgcat cgtgctcggc atgcaggtcg cattcgacgt aggcgatcac
     8341 gtcggccaga acgggggatc ccgttgcccc cggcgaccag tcgaggctgg caaacttttc
     8401 gacccccgat tgcgcgaaaa cgtcgcagac ggccttctga tcctcgccga gaatgttgat
     8461 gcagaagctg cccgagtccc tgagcttcgg ccagctggtg ctcgtcttgg cggggcacag
     8521 cacgaccagc ggcgggtcca gcgaaaccga gctgaaggac tgggcggcga aaccggccgg
     8581 ctcgccctct cggcagcccg tcgcaatcac cacccccgtg cagaactgtc ccatggtgtc
     8641 gcgaaacacc cgttggtcga tttccgacat cccgctcccc tgggatcgaa gagccgcgga
     8701 ctataacctt ccgctgctgc cctggccaac gagcccgttt cctttagaat tccgagtccg
     8761 gcagataggg cgcagcagag gttcagacag cggtgacaga tcttaacgag tttcgcaaag
     8821 aggtcagaaa ctggctagag gagaactgcc cgcccagcat gcgcacgtcc acgcccgagg
     8881 gcgagaccgt ctggggcggc cgccgggaga agttcgcgaa tccggactcc aggatctggc
     8941 tggaccgcat ggccgagcgc gggtggacgg cacccacgtg gccccgggag tacggcggcg
     9001 gcggccttac ggccgacgag gacaaggtgc tccagcagga actgcgcagc atcaatgcca
     9061 ggccggcgct gcagagcttc ggcatctgga tgctggggcc ggccctgctg gagttcgcca
     9121 gcgaggagca gaaactcgag cacataccgc agatcgttcg cggcgagatc cgctggtgcc
     9181 agggctactc ggagccgggc tacggctcgg acctggccgg gctgcagacc aaggcggagg
     9241 accagggcga ccacttcctg gtcaacggtt ccaagatctg gacctcctac gcggacgttg
     9301 cggactggat cttctgcctg gtccgcaccg acaacgacgc gccgaagcac gagggcatca
     9361 gctttctcct cttcgacatg gactccgagg gcgtgaccac ctcccccatc gagctgatca
     9421 gcggcgcctc gcccttctgc cagacgttct tcgacaacgt gagggtgccc aaggcaaacc
     9481 tggtgggc
//
LOCUS       DBNHOX010000563         2191 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_945905, whole genome shotgun sequence.
ACCESSION   DBNHOX010000563 DBNHOX010000000
VERSION     DBNHOX010000563.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_945905"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="AC16UZ_12375"
     CDS             complement(<1..402)
                     /locus_tag="AC16UZ_12375"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6247525.1"
                     /translation="MSNETRCCIVGAGPAGMFLALLLARRGVTVTVLELHRDLDRDFR
                     GDTVHASTLEVLDQIGLADRLLALPHAKMRQVTLHTAARTLELVDFSRLKTRFPYVMV
                     MPQVVFLNYLHEQAKSYPGFRCVLGAQVQQLV"
     gene            551..1243
                     /locus_tag="AC16UZ_12380"
     CDS             551..1243
                     /locus_tag="AC16UZ_12380"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007992649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR6247526.1"
                     /translation="MGATSALHAAEPEELLKRRLMAEIRGDAKRTADYTGRSALDERV
                     MAALEAVPRHEFVEAWSPGAAWENVPLPIGHGQTISQPFIVALMTDFVEPHPTHRVLE
                     IGTGSGYQAAVLAGLVGEVYTVEIIPALAQTATERLHRLGYDNVFVRVGDGFYGWPEK
                     GPFDGIVVTAVAPEIPAPLLEQLRPGGRMILPVGEVHGGQNLVLVSKDADGAVATRDV
                     LPVMFVPLTGDH"
     gene            complement(1255..2064)
                     /locus_tag="AC16UZ_12385"
     CDS             complement(1255..2064)
                     /locus_tag="AC16UZ_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007980046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6247527.1"
                     /translation="MTERYQGYQRLEFDYPHPRILRISFNRPERYNALDVVGHNEITY
                     VWREVDADPDIDCVILTGKGKAFSAGGDFEMIEEIIADFDTRAQTWKEARDLVYNIIN
                     CGKPIISAINGPAVGAGLVAGLLADISIVAESVSLTDGHTRLGVAAGDHAAIIWPLLC
                     GMAKAKYYLLTCDRLSGAEAERIGLVSMCVPDEELESRALSVAVQLANGAPNAIRWTK
                     YALNNWLRLAGPTFDASTALEMLGFTSGEAREGVSSLKEKRDPEFKKGSRI"
BASE COUNT          365 a          676 c          746 g          404 t
ORIGIN      
        1 aacgagctgt tgaacctggg cgccgagaac gcaccgaaat cccgggtagc ttttcgcctg
       61 ctcgtgaagg tagttgagga agacgacttg aggcatgacc atcacgtagg gaaagcgcgt
      121 cttcagccgc gaaaagtcca ccagctcgag cgtccgcgcg gcggtgtgga gagtgacctg
      181 gcgcatcttt gcgtggggca gcgcgagcag gcggtcggcc aggccgatct gatcgagcac
      241 ctccagcgtg gaggcatgca cggtatctcc gcggaagtcg cggtccaggt cgcgatggag
      301 ctcgaggacg gtgaccgtca ctcctcgccg ggccaaaaga agtgccagga acattcctgc
      361 ggggccggca ccgacgatgc agcagcgggt ttcgttgctc atgccgacag gctatgtcgt
      421 tgccgacggg cgcgcaaatc catacgggac aatgcggatg ggccggctcg gccagccatg
      481 tcatgctaaa tagatgtact gcgctgcatc gacagggctg cttgcctggg cgttcgggag
      541 tttcctcctg atgggggcga cgtccgcgct gcacgccgca gagccggaag agctgctcaa
      601 gcgccggctg atggcagaga tccgtggtga tgcgaagcgc acggcggact ataccggccg
      661 aagtgccctc gacgaacgtg tgatggcggc gctcgaggcc gtgccccgcc acgagtttgt
      721 cgaggcctgg agccccggcg cggcctggga gaacgtcccc ttacccattg gtcacgggca
      781 gaccatttct cagccgttca tcgtcgcgct catgaccgac ttcgtcgagc cccacccgac
      841 gcaccgggtg ctggagatcg gcacggggtc gggctatcag gcggccgtgc tcgcggggct
      901 cgtgggcgag gtttacacgg tggagatcat tcccgcgctc gcgcaaacgg ccaccgagcg
      961 gctgcaccgg ctcggctacg acaacgtctt cgtgcgcgtc ggtgacgggt tttacggttg
     1021 gcccgagaaa ggtccgttcg acggtatcgt cgtaacggcc gttgcgccag agatccctgc
     1081 gccgctgctg gagcagctgc ggcccggcgg tcggatgatc cttcccgtgg gcgaggtgca
     1141 tggcggtcag aacctggtgc tcgtcagcaa ggacgccgac ggagcggtgg cgaccaggga
     1201 cgtgctgccg gtaatgttcg ttccgctgac cggcgaccac tgacgccacc gacctcagat
     1261 ccgggaacct ttcttgaact ccggatcccg cttctccttg agtgagctga ccccctccct
     1321 cgcctcgccg ctcgtgaacc cgagcatctc gagtgccgtg gacgcatcga aggtcgggcc
     1381 ggccagacgc agccagttgt tcagcgcgta cttggtccag cgaatggcgt tgggagcgcc
     1441 gttggcgagc tgcacggcga ccgacagggc ccggctctcg agctcttcgt ccggcacgca
     1501 catggagacc aggccgatgc gctcggcctc ggcgccggac aaccggtcgc aggtcagaag
     1561 gtagtacttt gccttggcca tcccgcaaag aagcggccag atgatggcgg cgtggtctcc
     1621 ggccgcgacg ccaaggcgcg tgtgaccgtc tgtcaggctg acggactccg ccacgatgga
     1681 gatgtctgcg agaagcccgg cgacgagccc cgcgcccacc gcgggcccgt tgatcgccga
     1741 tatgatcggc ttgccgcagt tgatgatgtt gtagaccaga tcgcgcgctt ctttccaggt
     1801 ctgcgcgcgg gtgtcgaagt cggcaatgat ctcttcgatc atctcgaaat cgccgcctgc
     1861 cgagaaagcc ttgcccttgc cggtgaggat cacgcagtcg atgtcggggt cggcgtccac
     1921 ttcgcgccag acgtaggtga tctcgttgtg accgacgacg tccagggcgt tgtagcgctc
     1981 cgggcggttg aagctgatgc gcagtatccg tgggtggggg tagtcgaact ccagtcgctg
     2041 gtatccctgg tagcgttcgg tcatctggtt cccctcgtcc tgctgtcaat tggctcaggg
     2101 gaacggtacg ctttttggga gatctggcaa gtcgccacgc ttttgcgcac ggcggcacaa
     2161 tcgaccgggc gttgagctgc catcatcggc t
//
LOCUS       DBNHOX010000564        10313 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_624943, whole genome shotgun sequence.
ACCESSION   DBNHOX010000564 DBNHOX010000000
VERSION     DBNHOX010000564.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10313)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10313)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10313
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_624943"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="AC16UZ_12390"
     CDS             complement(<1..1148)
                     /locus_tag="AC16UZ_12390"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR6247528.1"
                     /translation="MLSDLRVLDLSTDLGLMCGQLLADMGAEVQQWVLPDDAARLDTC
                     HWQAYTLGKGVQLIDWQADPEAVRRQVGCVDVIIESAPPGLMARLGLGYEELARINPE
                     VVHVSITGFGSTGPKADYEFSDLISAAAAGLVYVTGSEGQAPVRTTCPQAHGHASSDA
                     AVGVLIALFERARTGRGQHVDVSAQQSTTLALLNRALDGPVGQAKAERSAYGMTINGV
                     HVQNQFQARDGWVVCLPGVLPPIAAFMERLMAWVFEEGLCREEDLRWDWGGVALQIMQ
                     GQVTPEQWAPVTAGMEALIAGRTKAELMSEAISRRLLIAPVFSVADLLDSDHLAQRGF
                     LVEGGAGLRLGPFARLSESPLPLTESGPRFWQERSAFDARPLAASPSGR"
     gene            complement(1138..2004)
                     /locus_tag="AC16UZ_12395"
     CDS             complement(1138..2004)
                     /locus_tag="AC16UZ_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247529.1"
                     /translation="MVAFRRCLVIALWCLSAHALGYDGRIHQDLTFLAAKHFNRCTAG
                     TEVPPLTPLQIRYLAKTNVGLADRNAFARMFNWRYYDRAAEAEHSVMWAIDTRFHEHF
                     NEILARIDKADSMGKLYSEVGRIINYLQLVSSPPHTVPVFTSRFWRFSFSDRFDGFPV
                     DVEAVGQAIGEDCSFLAAEEEYAGVLRSIAADTIAAVRAPIEGMPATWEAFWEIADDP
                     EKFGDYGPAGNSFGRKTEFGCAQQRCVLLRADPLYAAFAAQRHAAAVKATLAAMLIVQ
                     RSQIATEVAGAQ"
     gene            complement(1991..2494)
                     /gene="folK"
                     /locus_tag="AC16UZ_12400"
     CDS             complement(1991..2494)
                     /gene="folK"
                     /locus_tag="AC16UZ_12400"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR6247530.1"
                     /translation="MVLVGLGSNRGDSVGIVRAAMGRLRGFADGDTRCSDLWRTSPVD
                     CPPGSEDFINAAVSFRAAPGLTPESLLADLKALEAEYGRTGQRLRNAPRELDLDLLLF
                     DAETRDTADFVLPHPRAADRLFVLAPAAQVAPDVVWPEIGLTIRELLDELDSDERVVR
                     LEAHGCL"
     gene            2655..3737
                     /locus_tag="AC16UZ_12405"
     CDS             2655..3737
                     /locus_tag="AC16UZ_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HIR6247531.1"
                     /translation="MKAAVLREINKPLEIEDIQHGDPGPREVLVRTVAAGVCHSDLHF
                     QNGSYPYPLPAVLGHESAGIVEAVGSDVNYVKPGDHVITCLSAFCGHCEYCLTGHMSL
                     CQEPELQRPMDQPQRLNKGDENIWQFLNLSSFAEYMLVHEHAIAKIREDMPLDRAALI
                     GCGVTTGVGAVIHTAKVEPGSTVAVIGCGGIGLSAINGAAIAGAARIIAIDTVESKLD
                     LARKFGATDVVNARESDVIEAVREMTGGGVHYSFEAIGLKQTAEQSFKILQRGGTATV
                     IGMIPVGTNIELHGPEFLMERKMQGSNMGSNRFRVDMPRFVEFYLQGRLHLDDMISRR
                     IRLEDVNDGLAALETGELARSVIMFD"
     gene            complement(3758..5119)
                     /locus_tag="AC16UZ_12410"
     CDS             complement(3758..5119)
                     /locus_tag="AC16UZ_12410"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIR6247532.1"
                     /translation="MRHTLKCAVFGWLLLGSLTAAAALPLGLPGGEQLPSLAPMLDQA
                     TPAVVNIATYTTVQVRNPLLEDPFFRRFFNVPDQRRYRRTQSAGSGVVIDAGKGFIVT
                     NNHVVERADEISVTLADGRSMTAELVGSDPQVDLAVLKVEADDLQQIGFADSGELRVG
                     DFVVAIGNPFGLRQTVTSGIISALGRSGLGIEGYEDFIQTDASINPGNSGGALVDLAG
                     RLVGINTAILAPSGGNVGIGFAIPSNMVRAIMDQLIEHGEVRRGYLGLAVQGLNVDLA
                     EAFGVDRREGVVVVEVEPDSAAESGGLQAGDIITRVGDREIGKIADFHSQAAVTFVGD
                     QVRIEVLRDGQPQQVTLKIGEDELEKVRGRRIDRRLAGTQLQNFRNQDEPGMGAGVLV
                     LDIDPDSDAYRFGLRPGDILVAANRRAIVNLAELREVARLDDGQLLLRVYRSGQFGYV
                     AIR"
     gene            5250..5678
                     /locus_tag="AC16UZ_12415"
     CDS             5250..5678
                     /locus_tag="AC16UZ_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6247533.1"
                     /translation="MPIKNVFEAIQREKANIENLSVEELKQALADDPDLLLLDIREIQ
                     ERVDLGTIPGSVHVPRGMLEFWADPASPYFRDYFQEDRRTVVFCAGGGRSVLAVRALQ
                     DMGFTDVAHLEPGFGGWKKAAEPIEDVASSSRWVRRADDD"
     gene            5754..5829
                     /locus_tag="AC16UZ_12420"
     tRNA            5754..5829
                     /locus_tag="AC16UZ_12420"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5787..5789,aa:Gly,seq:ccc)
     gene            complement(5833..7614)
                     /gene="pabB"
                     /locus_tag="AC16UZ_12425"
     CDS             complement(5833..7614)
                     /gene="pabB"
                     /locus_tag="AC16UZ_12425"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - aminodeoxychorismate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008153 - 4-aminobenzoate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HIR6247534.1"
                     /translation="MSTDVIVYDAVAQQWLRFCDPVELLIADREDQVLDVVAEIESRT
                     VGDGLFAAGFLTYEAAPGIARELVTHRPHRLLPLAWFGLFRRVLPSADLPLAPGDCLG
                     GWSASVTRKDYDSAIARIRDYIAAGDTYQVNYSFRLRARLNDSPAALFANMVSSQGPG
                     LAAFIDTPGFAVASASHELFFARDGDELVSRPMKGTAPRGLRDQTDRDQAGWLAASAK
                     NRAENLMITDMVRNDLGRIARIGSVVADDLFALERYPTVWQMTSTVRARSTASLPDVM
                     GALFPAASITGAPKRRTMEIIRELEHEPRGIYTGTIGFVRPEGRCQFNVAIRTAAIDK
                     TTSEAEYGVGGGIVWDSRAEDEYRECFAKARIVTGGRREFELLETLAWHPRDGFRRLE
                     EHLERLHSAADYFCWSLSTGKAQALLEAAASAYREPMRVRLLADRSGHVRLEASPLKP
                     LPEPYRVALSEPMEDLQSEFVYHKTTQRSVYDKALDAARSVRPDLDDVLLVNGDGQVT
                     ESTIANVAVEVDGRLLTPELGCGLLPGIERQVLVADKILEEARVPVSLLVEAERIWLL
                     NSVRGMWPATLVATEQGGSDLRSEFPG"
     gene            complement(7736..8701)
                     /locus_tag="AC16UZ_12430"
     CDS             complement(7736..8701)
                     /locus_tag="AC16UZ_12430"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6247535.1"
                     /translation="MRPPALTWLILGSLLAVLSLPAISSEPARPLSAHHVIVDGVPLA
                     VWRKAPASAKRVVLLVHGRTWSARPNFDLNVPGEDLSLMDGLVEEGIATYALDMRGYG
                     DTPRDASGWLTPSRAAADVIAVLEWIAQRNPGLPGPYLFGYSRGAMVAQLAAQQQPGR
                     ISGLIVFGYPLRQDSAVNPPGLTTEPARIANSAADARSDFIAPGAARRETIDAFVESA
                     LKHDPVRVDWRAYPEWLALDAARVTVPTLLVDAEHDPLTDPKVLSRFFAGLATADKAW
                     VSIPGADHAAFLEEPRDYFRRAVAAFMLGERAAVEVISADLLDFD"
     gene            8859..9632
                     /locus_tag="AC16UZ_12435"
     CDS             8859..9632
                     /locus_tag="AC16UZ_12435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6247536.1"
                     /translation="MDVSGKIVVVTGGASGIGRAMAQRFHAEGAGKVIVADLDGDGAR
                     RVADEIDGASMQVDVGREADLVALIEHAESEFGPIDLFCSNAGIGIGKGLDEPDEVWD
                     TIWKVNTMSHVYAARHMVPRMKARGGGYLLNTASAAGLLTQIGSVTYAVTKHAAVALA
                     EWIAITHARDGIKVSVLCPQAVRTPMTADGPGVAGVDGMIEPEEAAECVIEALREERF
                     LVLPHPEVATYIQRKTSDYDRWLNGMRRLQDLYVAEEGE"
     gene            complement(9682..>10313)
                     /locus_tag="AC16UZ_12440"
     CDS             complement(9682..>10313)
                     /locus_tag="AC16UZ_12440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6247537.1"
                     /translation="LSGIEVSADRVCIGAFTVHDAVASSPEIRATLPVLAFLAGQIGD
                     GQVRNRGTLGGSVANSDPAADYPAALIGLGATVHTQTRSIEADDYFKDLFETALNPGE
                     LITRIDFPVPTRAAYRKFPNPASRYAVVGVLVADFGGKVRVGVTGAGPCAFRATAIEE
                     LLSRNLDPSALDGVEVPDAGFNNDLHASAEYRAHLVKVMAKRAVADLLS"
BASE COUNT         1850 a         3466 c         3361 g         1636 t
ORIGIN      
        1 cggccggacg gggaggcagc caggggtctg gcgtcgaaag cagacctttc ctgccagaat
       61 cgcggcccgc tttcggtcag cggcagcggc gactctgaca aacgggcaaa cggcccgagc
      121 cgaaggcccg ctccgccctc caccaggaag ccgcgctgag cgaggtgatc gctgtccaga
      181 aggtccgcta cagagaacac cggagcgatg agcagccgcc ggctgatggc ctcggacatc
      241 agctctgcct tggtgcgccc agcgatcagc gcttccatgc cggcagtcac cggggcccat
      301 tgctccgggg taacttgccc ctgcatgatc tgaagggcca cgccgcccca gtcccacctg
      361 aggtcttcct cgcggcaaag gccttcttcg aacacccacg ccatcagccg ctccatgaaa
      421 gcggcaatgg gtggaagcac acccggcagg cataccaccc agccgtcacg ggcctgaaac
      481 tggttctgaa cgtgcacgcc gttgatcgtc atgccgtaag cgctgcgctc cgccttggcc
      541 tggccaacgg gaccgtcaag cgcccgattg agaagcgcca gcgtggtcga ctgctgggca
      601 gacacatcga cgtgctgacc gcggcccgtt cgcgctcgct cgaagagcgc aatgagcacg
      661 cccaccgccg catcgctcga ggcgtgcccg tgggcctgcg gacacgtcgt gcgaacgggc
      721 gcctgccctt cgctgcccgt cacgtagacc agccccgctg cagccgcgga aatcagatcc
      781 gagaactcgt aatccgcctt cgggccggtg cttccgaacc cggtgatgga gacatgaacg
      841 acctccgggt tgatgcgcgc gagctcctcg tagccgaggc ccaggcgcgc catgagcccg
      901 ggcggggcgg actcgatgat cacgtcgacg cagccgacct gccggcgaac ggcctcggga
      961 tcggcctgcc agtcgatcag ctgaacgcct ttgccgagcg tgtaggcctg ccagtggcag
     1021 gtgtcgagtc gggccgcatc gtcgggcagg acccattgct gaacttccgc ccccatgtcg
     1081 gcgagcagct gcccgcacat caacccgaga tccgtggaga gatcgagcac gcgaagatca
     1141 ctgagcaccg gcaacctccg ttgcaatctg gctgcgctgc acgatgagca tggctgccag
     1201 ggtagctttc accgccgccg cgtgccgttg ggcggcaaac gcggcgtaga gcgggtcggc
     1261 gcgcaggagc acgcagcgct gctgcgcgca gccgaactcg gtcttgcggc caaagctgtt
     1321 gccggccgga ccgtagtcac caaacttctc cggatcgtcg gcgatttccc agaaggcctc
     1381 ccaggtggcc ggcatgcctt cgatgggcgc gcgtaccgcc gcgatggtat ccgcggcgat
     1441 ggagcggaga acccccgcgt attcctcctc ggcggcgagg aaggaacagt cctcgccgat
     1501 ggcctgcccc acggcttcca cgtccaccgg gaatccatcg aagcggtcgc tgaagctgaa
     1561 ccgccagaac cgcgaggtga ataccggcac ggtgtgaggc ggggacgaca ccagctgcag
     1621 gtagttgatg atccggccga cctcgctgta cagcttcccc atgctgtcgg ccttgtcgat
     1681 gcgcgcaaga atctcgttga aatgctcgtg aaagcgcgtg tcgatcgccc acatcaccga
     1741 gtgctccgcc tcggcggccc ggtcgtagta gcgccagttg aacatgcggg cgaacgcgtt
     1801 ccgatccgcg aggcccacgt tcgtcttggc gaggtagcgg atctgcagcg gcgtcagcgg
     1861 cgggacctcg gtgcccgcgg tgcagcggtt gaagtgcttt gccgccagaa aggtcaggtc
     1921 ctgatgaatc cggccatcgt acccgagcgc atgggccgac aggcaccaga gcgcgattac
     1981 caggcaacgc ctaaaggcaa ccatgcgctt ccagacgcac cacgcgctcg tcgctgtcga
     2041 gctcgtcgag cagctcccgg atcgtcaacc cgatttccgg ccagaccacg tccggtgcca
     2101 cctgcgccgc cggcgcgagc acgaagaggc gatcggcggc gcgcgggtgc ggcaggacga
     2161 agtcggccgt gtcccgcgtc tcggcgtcga acagcagcag gtcgagatcc agctcccggg
     2221 gggcgttacg caagcgctgc ccggtccggc catattcggc ctcgagcgct ttgagatcag
     2281 caagcaggct ctccggcgtc aacccggggg cggcacgaaa ggagaccgcc gcgttgatga
     2341 agtcttccga ccccggtggg caatccaccg gcgacgtgcg ccacagatcg gagcagcgcg
     2401 tgtcgccgtc ggcaaaccca cgaagcctgc ccatggcggc gcggacgata ccgacgctgt
     2461 cgccccgatt ggatccaaga ccaaccaata ccatcgtttt tccggtgtcc tagcaggcat
     2521 cccccagggc caatactaac ctctgctatc ccccggtagt aagggagatc gggcgccggg
     2581 accggcggca gatggtagta atcggaccat gcgtttggca cactcaaacg tcggacacgt
     2641 acagggagag gcccatgaaa gccgccgttt tgcgcgagat caacaagccg ctggaaatcg
     2701 aagatatcca gcacggcgac ccgggcccac gcgaggtgct ggtgcgcacg gtggcggccg
     2761 gcgtgtgcca ttcggatctg cacttccaga acggctcgta tccatatccc ctgccggccg
     2821 tgctcggcca tgagtccgcc ggcatcgtcg aggccgtggg cagcgacgtc aactacgtca
     2881 agcccgggga tcatgtgata acgtgtcttt cggcgttctg cggtcactgc gagtactgcc
     2941 tgaccggcca catgtcgctg tgccaggagc ccgagctgca gcggcccatg gaccagccgc
     3001 agcgcctcaa caagggcgac gagaacatct ggcagttcct gaacctgtcc tcgttcgccg
     3061 agtacatgct cgtccacgag cacgccattg cgaaaatccg ggaggacatg cctctggatc
     3121 gggccgccct catcggctgt ggcgtgacga cgggcgtcgg ggccgtcatt cacaccgcca
     3181 aggtggagcc gggcagcacg gtcgccgtga tcggctgtgg cggcatcggg ctctccgcca
     3241 tcaacggggc cgccatcgcc ggcgccgccc ggatcattgc catcgacacc gtggaaagca
     3301 agctcgatct ggcgcgcaaa ttcggcgcca ccgacgtcgt gaatgccagg gagagcgacg
     3361 tcatcgaggc ggtgagagag atgaccggtg gcggcgtgca ctactccttc gaggccattg
     3421 gtctcaagca gacggccgag cagtctttca agatcctgca gcgcggcggc accgcgaccg
     3481 tcatcggcat gattcccgtg ggcacgaaca tcgagctgca tggacccgag ttcctcatgg
     3541 agcgcaagat gcaggggtcg aacatggggt ccaaccgatt ccgcgtggac atgccgcgat
     3601 tcgtggagtt ctacctgcag ggccgcctgc atctggacga tatgatctcc cgccggatcc
     3661 gcctggaaga cgtcaacgac ggcctggccg ctctggagac cggagagctg gcgcgcagcg
     3721 tcatcatgtt cgactaaacg cccggccggg ccgccggtca gcgaatggcg acgtacccga
     3781 actggcctga gcggtacacc cggagcagca gctgaccgtc gtcgagcctg gcgacttccc
     3841 tcagctctgc gaggttgacg atggcccgac ggttggcggc cacgagaatg tctccaggtc
     3901 gcagcccgaa ccggtaagcg tcgctgtccg ggtcgatgtc gagcaccagc acgccggcac
     3961 ccatgcccgg ttcgtcctgg ttgcggaagt tctgcaactg cgttcccgcc aaccggcgat
     4021 cgatgcggcg gccgcgcacc ttctccagct cgtcctcgcc gatcttgagg gtgacctgct
     4081 gcggctggcc gtcgcgaagc acctcgatcc ggacctggtc cccgacgaac gtcaccgccg
     4141 cctggctgtg gaaatcggcg atcttgccga tctcccgatc gcccacgcga gtgatgatgt
     4201 caccggcctg aagcccgccg gactccgcgg cgctgtccgg ctcgacctca accacgacca
     4261 caccttcgcg gcggtcgacg ccgaacgctt ccgcgagatc cacgttcagc ccctgaacgg
     4321 ctagaccgag gtagccgcgg cgcacctcgc cgtgctcgat cagctgatcc atgatcgccc
     4381 ggaccatgtt cgagggaatg gcgaagccga tgccgacgtt tccgccgctc ggcgccagga
     4441 tggcggtgtt tatccccacc agacgcccgg ccaggtccac gagggcgccg ccggaattgc
     4501 ccgggttgat ggacgcatcg gtctggatga agtcctcgta cccctcgatg cccaggccgc
     4561 tgcgacccag cgcgctgatg atcccgctcg tcaccgtctg ccgcagcccg aagggattgc
     4621 cgatggccac gacgaagtcg cccacccgca gttcgccgga gtccgcaaag ccgatctgct
     4681 gcaggtcgtc cgcctccacc tttagcaccg ccagatcgac ctggggatcg ctgccgacga
     4741 gctcggccgt catcgaacgg ccgtcggcca gcgtcaccga gatctcatcg gcccgttcga
     4801 ccacgtgatt gttggtcacg atgaagccct tgccggcatc gatcaccacg ccagagcccg
     4861 cactctgggt tcgccggtaa cggcgctggt cagggacgtt gaagaaccgg cggaagaacg
     4921 gatcctccaa cagcgggttg cggacctgta ccgtggtgta ggtggcaatg ttgaccacgg
     4981 cgggcgtggc ctggtcgagc atgggcgcaa ggctgggaag ctgctcgccg cccggcagcc
     5041 ccagcggcaa agccgcagcg gccgtcaagc tgcccagcag cagccacccg aaaaccgcgc
     5101 acttcagagt gtgcctcaat tgaccgttcc tcaccttggt tggtagcacc gatgttggac
     5161 agcgcccggt tgccggcagt tccaccagga cggggcggcc ggcagggact cagtgtacac
     5221 tcactgctcg cattcggggg agcacggacg tgccgatcaa aaatgtcttc gaagccatcc
     5281 agcgcgagaa ggcgaacata gagaacttga gcgtcgagga gctcaagcag gctcttgcag
     5341 acgaccccga cctgctgctg ctcgacattc gcgagatcca ggagcgcgtg gaccttggca
     5401 ccatacccgg ttccgttcac gtccccaggg gcatgctgga gttctgggcc gacccggcta
     5461 gcccttactt tcgggactat ttccaggaag accggcgcac cgtggtgttc tgcgccggcg
     5521 gcggccgctc cgtgctggcc gtcagagccc tgcaggacat gggctttacc gacgtggccc
     5581 atctcgagcc cggcttcggc ggctggaaaa aagccgccga gcccatcgaa gacgtggctt
     5641 ccagcagccg ctgggtacgg cgggccgacg acgactgaag ccgtgccgct tgcgggacca
     5701 tcacctcggg atggtcaccg gcgagcgctc gtgccagaat cgacaacctc tcggcgggac
     5761 tagctcatct ggtagagcga cagcttccca agctgtaggc gacgggttcg agtcccgtgt
     5821 cccgctccat cttcaccccg ggaattccga gcgcagatcg ctgccaccct gctcggtcgc
     5881 gaccagcgtc gccggccaca tcccccggac ggagttgagc agccagatgc gctccgcctc
     5941 gacgagcagg ctaaccggaa cgcgcgcttc ttcgaggatt ttgtcggcga cgagcacctg
     6001 acgttcgatc ccgggaagca ggccgcagcc gagctcgggc gtcagcaggc ggccgtcgac
     6061 ctccacggcc acgttggcaa tggtcgactc cgtcacctgc ccgtcgccgt tgaccagcag
     6121 cacatcgtcg aggtccggcc ggacgctgcg ggccgcgtcg agcgccttgt catacaccga
     6181 acgttgcgtc gtcttgtggt acacgaactc gctctgcaaa tcctccatcg gttcggatag
     6241 ggccacacgg tagggttcgg gcaaaggttt cagcggcgaa gcctctagcc ttacgtgacc
     6301 cgaacggtcg gcgagcaggc ggacgcgcat gggctcgcga tacgccgacg cggccgcttc
     6361 gagcaaggct tgcgcctttc cggtgctcag cgaccagcag aaatagtcag ccgcgctgtg
     6421 cagcctctcg agatgctcct cgagacggcg aaacccgtca cgggggtgcc aggccagggt
     6481 ttcgagcagc tcgaattccc ggcgcccacc ggtgacgatg cgcgctttcg cgaagcactc
     6541 ccgatactcg tcttcggctc gggagtccca gacgatgccg ccacccacac cgtactccgc
     6601 ctcggacgtc gtcttgtcga tggcggcggt gcgaatagcc acgttgaact ggcagcgccc
     6661 ctcaggacgc acgaagccaa tggtgcccgt gtagatcccg cgcggctcat gctcgagctc
     6721 gcgaatgatc tccatggttc tgcgtttggg cgctccggtg atggaggccg ccgggaacag
     6781 ggcgcccatg acgtccggca ggctggcggt cgatcgtgct cttaccgtgc tggtcatctg
     6841 ccagacggtg gggtaccgct ccagggcaaa gaggtcgtcg gcaaccaccg atccgatcct
     6901 ggcaatgcgg ccaaggtcgt tgcggaccat atcggtgatc atcaggtttt cggcacgatt
     6961 ctttgccgat gcggccagcc agccggcctg atcccgatcc gtttggtccc gcagaccgcg
     7021 cggcgcggtg cccttcattg gccgggaaac gagctcgtcc ccgtcgcggg cgaagaacag
     7081 ctcgtgggat gcgctggcaa cggcgaaccc gggcgtgtcg atgaaagcag ccaggccagg
     7141 gccctgagag ctcaccatgt tcgcgaacaa ggcggcgggg gaatcgttca gacgagcgcg
     7201 caggcgaaac gagtagttca cctgataggt gtcaccagcc gcgatgtagt ctcgaatccg
     7261 ggcgatggcc gaatcgtagt ccttgcgagt gacggacgcc gaccatccgc caaggcagtc
     7321 gcccggggca agaggaaggt cggcgctcgg cagaacccgc cggaacagac cgaaccatgc
     7381 gagcggaagg agccggtgcg gcctgtgcgt caccagctcg cgcgcaattc ccggagcggc
     7441 ttcataggtc agaaatcccg ccgcgaagag gccgtcgccc accgtgcggc tctcgatctc
     7501 ggcgacgacg tcaaggacct gatcctcgcg gtccgcgatc agcagctcaa cgggatcgca
     7561 aaagcgcaac cattgttgcg cgacggcatc gtagactatg acgtctgtcg acatgggctt
     7621 gcggatcagg gtggccgggt atgctaaccg cggtcagctg cgagactcaa tgcgccattc
     7681 ccggacaggc ggcccctacc caccctgggc agatgcggta ggcaccaaag aagggtcagt
     7741 cgaagtcgag cagatccgcg gagatcacct ctaccgctgc ccgttctccc agcatgaacg
     7801 cggccaccgc ccgacggaag tagtcccggg gctcttctag aaacgccgca tgatccgccc
     7861 cgggtatgct cacccaggcc ttgtccgccg tggccagccc cgcaaaaaag cgcgagagaa
     7921 ccttggggtc cgtgagcggg tcgtgctcag cgtccacgag cagcgttggc acggtgacgc
     7981 gggcggcgtc cagagccagc cactccggat aggcgcgcca gtcgacgcgc accggatcgt
     8041 gcttgagcgc gctctccacg aacgcatcga tcgtttcccg gcgggcagcc ccgggggcaa
     8101 tgaaatccga cctggcatcc gctgcggaat tggcgatccg cgccggttcg gtggtcagtc
     8161 ctggtgggtt caccgccgag tcctgccgca aggggtagcc gaaaacaatg agccccgaga
     8221 tccgcccggg ctgctgctga gcggcgagtt gggccaccat ggcccctcgg gagtatccga
     8281 acaggtacgg cccggggagg ccggggttgc gctgggcaat ccattccagc accgcgatga
     8341 cgtccgcagc ggcccgggac ggggtgagcc atccggaggc atcccggggc gtgtccccgt
     8401 agccgcgcat gtccagcgcg tacgtggcaa taccctcctc gaccagaccg tccatgagag
     8461 aaagatcctc gccgggcacg ttcagatcga agttgggccg cgcgctccac gtgcgcccgt
     8521 gaacgagcag cacgacgcgc ttggcgctgg ccggcgcttt ccgccatacc gcaagcggca
     8581 cgccgtcgac gatcacgtgg tgggccgaga gcggtcgcgc cggctcgctc gagatcgccg
     8641 gcagcgaaag aaccgccagc agcgacccca ggataagcca cgtcagggct gggggccgca
     8701 tcgtcaactt gctcctcatg gtctgcgtcc aaactccggg tcattcggcg acgttgcacg
     8761 ccccccggac cgcgcaatcc ttcgcgacta tagattcaac gttctggaac gtctaatgtt
     8821 cagagcatca aaacaacgcg gttcacgggg gaaacgagat ggacgtctcg ggcaagatcg
     8881 tcgtcgtcac cggcggcgcc agcggtatcg ggcgtgccat ggcgcagcgc tttcatgccg
     8941 agggcgcagg caaggtgatc gtcgccgacc tcgacggcga cggcgcccgc cgcgtggccg
     9001 acgaaataga tggtgcgtcc atgcaggtag acgtcggcag ggaagcagac ctcgtcgcgc
     9061 tgatcgagca cgctgaatcc gagttcgggc cgatcgatct gttctgttcc aacgcgggaa
     9121 tcgggatcgg caaaggtctc gacgagcccg acgaggtgtg ggacaccatc tggaaggtca
     9181 acaccatgtc ccacgtctac gccgcccgcc acatggtgcc gcgcatgaag gcgcgcggag
     9241 gcggttatct gctcaatacc gcctcggccg cgggcctgct gacacagatc ggctcggtca
     9301 cctacgcggt gaccaagcac gccgcggttg cgctggccga gtggatcgcg atcactcacg
     9361 ctcgcgacgg cataaaagtc tccgtgctct gcccccaggc cgtgcgcacc ccgatgacgg
     9421 ccgatggccc gggcgtggct ggcgtggacg gcatgatcga acccgaggag gccgccgaat
     9481 gcgtgatcga agcgctgcgc gaagagcgct tcctggtgct gccgcatccg gaggtcgcca
     9541 cctacatcca gcgcaagacg tccgattacg accgctggct gaacggcatg cgccgccttc
     9601 aggatctgta cgtcgccgaa gaaggcgagt gagcgctgcc gccgacgccg ccggctgagc
     9661 cagccgcccc ggtacgaagc ttcagctcag caggtccgcc acggcccgct tcgccatcac
     9721 cttgaccagg tgcgcccggt actcggcgga ggcgtgcagg tcgttgttga atcccgcatc
     9781 cgggacctcg acgccgtcca gcgccgacgg gtcgagattg cggctgagca actcctcgat
     9841 agcagtggcg cgaaaggcgc agggacccgc accggtcacc cccacgcgga ccttgccgcc
     9901 gaagtcggcc accagcacgc ccaccacggc gtagcgggaa gcagggttcg ggaacttgcg
     9961 gtaggcggcc cgggtcggca cgggaaagtc gatccgcgta atcagctctc cggggttgag
    10021 ggccgtctcg aagagatctt tgaagtagtc gtccgcttcg atgctgcgcg tctgagtatg
    10081 caccgtggcg cccagaccga tcagcgcagc ggggtaatcg gcggccgggt cgctgttggc
    10141 gacggatccc cccagcgtgc ctcggttgcg cacctggccg tcgccgatct ggccagccag
    10201 gaaagccagc acgggaaggg tcgcgcgaat ctccggcgag ctcgccaccg catcgtgaac
    10261 ggtgaacgcg ccgatgcaga cccggtcggc gctcacctcg atgccgctga gcg
//
LOCUS       DBNHOX010000565         4360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_239132, whole genome shotgun sequence.
ACCESSION   DBNHOX010000565 DBNHOX010000000
VERSION     DBNHOX010000565.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_239132"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="AC16UZ_12445"
     CDS             complement(<1..621)
                     /locus_tag="AC16UZ_12445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional activator/DNA
                     repair enzyme AdaA"
                     /protein_id="HIR6247538.1"
                     /translation="MLPAPEICERARLARDPRFDGRFLTGVVSTGIYCRPICPARPAG
                     RDNVRYFPTAAAAEAQGYRACRRCRPEVAPGVPEWAISSDRVIRGLRLIDGGFLADQS
                     TAALAARLGVSTRHLNRLFHQELGATPSSLARSRRLHMALRLIVETDLGFCDVALQAG
                     YGSVRRFNAEVSGTFGRSPRALRGVRDEELTRELTLKLPVREPFDAP"
     gene            691..1908
                     /locus_tag="AC16UZ_12450"
     CDS             691..1908
                     /locus_tag="AC16UZ_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247539.1"
                     /translation="MSKAPEQPAIDYPDSDAGLDTALATWLQDPGGADAYRQDISFRP
                     PGGAHAAPGAVEQIAAQYRFARGEHGVSVTASLDGRSLSLDAPVRLEPVSIPKPWGRE
                     LWYTGMEVRGESRVVAGEASLPLSRYLALAPARLCRRRPIVLLKILDPAATPVAGDLY
                     FEVHEHKREVYVVTHVDPTAWPDGTGAIRLGMSQSRRAAFAEDDAFRNAYLAAVKAYE
                     TVRRRIDGGERLPPETETQLREAMDGFTAFAPLRVGDVVTVHPWQPHSLQHGVRVVEF
                     QTPTYERYILSFGQRVLTQNHWDTEAVLDRIDLGPPRPESLGPVAAGIGQIADFDDFS
                     AFRVEIPPGVSRPLEIELPYAVCMGVEGRVSVNGLALDAEEACFVPASALPVTLANAA
                     ATPAVALVAAAKL"
     gene            1912..2991
                     /locus_tag="AC16UZ_12455"
     CDS             1912..2991
                     /locus_tag="AC16UZ_12455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6247540.1"
                     /translation="MEATRKIRVRLWRRIAPIVVVSAFYYGVLVYPVLRILGLAWPDW
                     QPGTWQLLAIMVGPLAGRLAYEAIPCSATRWLSALALTWLGICFVAFTLVLGWEVANL
                     LLPLPGRESGWALLGLTGFACLVGYVNAQRVAVRNIDVPAPQIGKRLRVAQISDVHVG
                     SRSGRFLRRVVKRTNAQDPDLVFITGDLVDFRDISQQELGSLSDLEAPAYFVIGNHER
                     YVDCEAICDRLRNLGVHVIRNDTVTIDRIQLVGIDDAEPKTQVGKILQTLEPDPHRYR
                     ILLYHRPDGARDAAAWGVNLMLCGHTHAGQIVPFNLLVKRVFPRIRGVYRVDGLTLYV
                     SPGTGTWGPILRLGSRSEISMMTLG"
     gene            3014..3694
                     /locus_tag="AC16UZ_12460"
     CDS             3014..3694
                     /locus_tag="AC16UZ_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6247541.1"
                     /translation="MAEEQELIVEQIEIGPMQNFTYIVGSRSAREVAIVDPAWDVEHL
                     VRHIEEKGYKLTAALVTHYHPDHIGGSFGGHNIDGIAELLAINPLKVYAHKEEAEGVR
                     KVTGISESDLVRVESGDDLRIGDVSIEFLHTPGHTPGSQCFRVHNTLVSGDTLFIQGC
                     GRVDLPGSNSEDMYHSLQKLKSLPDETLLLPGHNYAHVPNATMGETKKFNTYMHVKDL
                     QTWRMIMG"
     gene            3832..4083
                     /locus_tag="AC16UZ_12465"
     CDS             3832..4083
                     /locus_tag="AC16UZ_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247542.1"
                     /translation="MNAYDHLVGQHFLRAGVRYTVVKCVGSTAITAYLRDNRIYRVVM
                     RVSDVLDLLEVTEISITVPAMEPKKALPAPQLERASGQS"
BASE COUNT          761 a         1475 c         1384 g          740 t
ORIGIN      
        1 cggcgcatcg aaaggctcac ggaccggcag cttgagggtc agctcccggg tcagttcttc
       61 gtcacgcacc ccgcgcagag cccgaggcga acgtccgaac gtcccgctca cctcggcgtt
      121 gaagcgccgg acgctgccgt agcccgcctg cagggcaaca tcgcagaagc ccaggtcggt
      181 ctcgacgatg agccggagag ccatgtgcag ccgccgggac ctcgccaggc tgctgggcgt
      241 ggccccgagc tcctggtgaa agagcctgtt gaggtgccgg gtgctcacgc cgagcctggc
      301 tgcgagcgcg gccgtgctct gatcggccag gaatcccccg tcaatcagcc gcagccccct
      361 gatcaccctg tcgctggaga tcgcccactc cggcaccccc ggcgcgacct ccggccgaca
      421 gcggcgacat gcccgatacc cttgggcttc cgccgccgca gccgtaggaa agtagcgcac
      481 gttgtcccgg ccggcgggcc gggccgggca gatgggcctg caatagatcc cggtgctgac
      541 gacgccggtg aggaaccggc cgtcgaagcg ggggtctctg gccaggcggg cgcgctcgca
      601 gatctctggg gcaggtaaca tgctggcctc ttcagcagct tccccgacta taatcccggc
      661 ctcgcgaaaa aactcgccgg aatcggacat ttgtccaaag cgcccgaaca gcccgccatt
      721 gactatcccg acagcgacgc cgggctggac acggccctgg ccacttggct gcaggatccc
      781 ggtggggcgg acgcctatcg tcaggacatt tccttccgac cgcctggggg cgctcacgct
      841 gctccgggcg ctgtcgagca gattgccgcg caataccgat tcgcccgagg cgagcacggc
      901 gtgagcgtga ccgcgtcgct ggatggacga agcctgtccc tcgatgcccc cgtccgcctg
      961 gagccggtca gcataccgaa accctggggc cgggaactgt ggtacacggg tatggaggta
     1021 cgcggcgaaa gtcgcgtggt tgccggcgag gccagcctgc ccctgtcgcg ctatcttgcg
     1081 ctcgcaccgg cgcgcctgtg ccggcgtcgt ccgatcgtcc ttttgaaaat tctggatcct
     1141 gccgccacgc ccgtggcggg cgacctctat ttcgaggtgc acgagcacaa gcgcgaggtg
     1201 tacgtcgtca cccacgtgga cccgaccgca tggccagacg gcacgggggc gatccggctc
     1261 gggatgagcc agtcgcgacg cgcagctttt gccgaggacg acgcgttccg aaacgcctac
     1321 ctcgcggcgg tgaaggccta cgagaccgtt cgccgccgca ttgacggggg cgaacggttg
     1381 ccgccggaaa ccgagacgca gctgcgcgaa gccatggacg gcttcacggc ctttgcgccg
     1441 ctgcgggtcg gtgacgtggt gaccgtccac ccctggcagc cgcattcgct gcagcatggt
     1501 gtgcgcgtcg tcgagttcca gaccccaacc tacgagcgct acattctgtc gttcggccaa
     1561 cgggtgctca cccagaacca ctgggacacc gaggccgtcc tcgaccggat cgatcttggc
     1621 cctccccggc cggagagttt ggggccggtc gccgccggca tcgggcagat cgcggacttc
     1681 gacgatttct ccgcctttcg ggtggagatc cctcccgggg tctcccgccc gctggaaatc
     1741 gagctgcctt acgccgtgtg catgggggta gaaggccgcg tatcggtgaa cgggctcgcc
     1801 ctggatgcag aagaggcctg cttcgtgccc gcctcggcac tccccgtgac cctggcaaac
     1861 gctgccgcca ccccggccgt tgctctggtg gcggcggcca agctctgagc catggaggcg
     1921 acgcgcaaga tccgggtcag gctgtggcgg cgcatcgcac ccatcgtcgt cgtttcggcc
     1981 ttctactacg gcgtcctggt ctatcccgtt ctgcgcattc tcggcctggc atggcccgac
     2041 tggcagccgg gcacatggca gctgctcgca atcatggtgg gtcccctcgc gggacgcctg
     2101 gcctacgagg ccataccctg cagcgcgacc cgctggctgt cggcgctggc gctgacctgg
     2161 ctggggatct gcttcgtcgc gttcaccctg gtgctcggct gggaggtggc caacctgctg
     2221 ctgccgctgc cgggtcgcga atccggctgg gcgcttctgg gactgaccgg gtttgcctgc
     2281 ctggtgggct acgtcaacgc gcagcgcgtg gcggtcagga acatcgacgt tcccgcacca
     2341 cagatcggca agcgcctgcg cgtagcgcaa atcagcgacg tgcacgtcgg ctcccgcagc
     2401 ggccggtttc tccgccgggt ggtgaaacgc acgaacgccc aggatccgga cctggttttc
     2461 atcaccggcg acttggtgga cttcagggac atctcccagc aggagctggg ctccctgagc
     2521 gacctcgaag cgcccgccta cttcgtcatc ggcaaccatg agcgctacgt cgactgcgag
     2581 gccatctgcg atcgcctgag gaatctcggg gtgcacgtga tacgcaacga caccgtgacg
     2641 atcgacagga ttcagctcgt cggcatcgac gatgccgagc cgaagaccca ggtcggcaag
     2701 atcctgcaga cgctcgagcc cgaccctcac aggtaccgga tactgctgta tcaccgtccg
     2761 gacggggcgc gggacgcggc ggcctggggc gtcaacctca tgctctgcgg gcacacccac
     2821 gcggggcaga tcgtgccgtt caatctgctc gtcaaacgag tattcccacg catccggggc
     2881 gtttaccggg tggatggttt gaccctttac gtctctccgg gcaccggaac gtggggcccc
     2941 attctccgct taggatctcg ttcagagatc agtatgatga cccttggctg aagaaacgac
     3001 cggacaagca atcatggcag aagagcagga actcatcgtc gagcagatcg agatcgggcc
     3061 catgcagaac ttcacgtaca tcgtggggtc tcgatcggcg cgcgaggtgg ccatcgtgga
     3121 cccggcatgg gacgtggagc acctcgtgcg ccacatcgag gaaaagggct acaagctcac
     3181 cgcagcgctg gtgactcact accatcccga tcacatcggc gggtcctttg gcgggcacaa
     3241 catcgacggc atcgccgagc tgctcgccat caatcctctc aaggtctacg cgcacaagga
     3301 ggaagccgaa ggagttcgca aggtcacggg catatccgag tccgatctcg ttcgcgtgga
     3361 gtccggcgac gatctgcgca tcggagacgt gagcatcgag ttcctgcaca cgcccgggca
     3421 cacgcccggc tcccagtgct ttcgagttca caacacgctg gtgtccggcg acaccctctt
     3481 cattcagggc tgcgggcgcg tggaccttcc cggatcgaac tccgaggaca tgtaccacag
     3541 ccttcagaaa ctcaaatcgc tccccgacga gacccttctt ctgccgggac acaactacgc
     3601 ccacgtcccc aacgcaacca tgggcgagac gaaaaaattc aacacctaca tgcacgtcaa
     3661 ggatctgcag acctggcgca tgatcatggg ctgacgcgcc tgccgccgat cggccggttt
     3721 ccgccattcg tcatcgccgc tcgccgggcg gccgctaaac cccgactgcc ggtcgtgcgt
     3781 cccggcgatc cgcgccgcta agattccgta ggagttgaag aggaattcga cgtgaacgcc
     3841 tacgatcatc ttgttggcca gcatttcctg cgcgccggag tgcgctacac cgtggtcaag
     3901 tgtgtcggct cgaccgccat caccgcctat cttcgcgaca accggatcta ccgggtcgtg
     3961 atgcgcgtga gcgacgttct cgatctcctg gaagtgacgg agatcagcat caccgttccc
     4021 gcgatggagc ccaagaaagc cctgccggca ccgcagctcg agcgcgcatc cggccagtcc
     4081 tgacaaggcg ccgccctggg tatcccaggc cggacctttc ctcccggttc cgccaagggg
     4141 gcatgagcct gccaacgcgg ctagtctcaa aggccggtta acgaaccgct tacatcactg
     4201 cgatatcagt tgagctcgga cacctataga ttaagccgat gagagagcag ccggtatttg
     4261 agacgcaggg cctgtgggtc cggtggagct gggacgagaa gcgcaagtcc ctgaagctcg
     4321 agaaggcgga gccacgtcct caaccggcca ccgctcaacc
//
LOCUS       DBNHOX010000566         9724 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_367563, whole genome shotgun sequence.
ACCESSION   DBNHOX010000566 DBNHOX010000000
VERSION     DBNHOX010000566.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9724)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_367563"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..2827
                     /locus_tag="AC16UZ_12470"
     CDS             80..2827
                     /locus_tag="AC16UZ_12470"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR6247543.1"
                     /translation="MRSVFRFMCFMLFVSAGPASLARGAVDVDQLAIPHETFVLENGL
                     TVIVHEDHSVPIVAVNLWYHVGSRNESRGRTGFAHLFEHFFFNGSEHYPHDFRRAMDD
                     VGANNRNGTTNPDRTNFFEDVPVSALERTLYLEADRMGFLAANITQETLDQERGIVQN
                     EKRQGENQPYGKVWNRIVESIYPYSHPYSWPIIGSMADLEAATLDDVKAWYAKYYGPS
                     NCVLSLAGDITVEQARELVGKYFAGIPPGPPVERYRQWVPTLERPLRDVMHDRVPQAR
                     IYRVYHTPGWLDAESVLIELNAGLLSGSRSAPLDRALVYDKQVATEVAAFAWDKEIAG
                     NLVVVVSVKPGVDPADAERELDAVMAQFLGQKPARSALDRARTRQVAQFIRGMERLGG
                     FGGRSDILAESMTYAGDPEAFLQRYRKVMEATPAELHAASTKWLDANHYTLTVLPFPE
                     LKAAAEDVDRTSLPALGPAPEVAFPEIGSRELDNGLRVLLLERRGAPLVNMSLAVDAG
                     YAADPAAAAGTASLALQLLEEGTRSRDGFALADDLDALGATISSASSLDLSFVQLRAL
                     KANLARSLAVFADVVRNPAFAGDMVALGKRRRLAQIAQEEAQPFSAALRMVPPLLYGD
                     GHVYAKPLTGSGYVETVRNIEPDDLSKWHSAWFRPNNATLIVTGDVTFDDLMPMVDDV
                     LGDWKPGAVPLKRVADVPRGKAGRIYLVDRPDASQSVIVAAQASIPGGQAEEVAIEAA
                     MRAFGGMSTSRFNQNLRIDKHWSYGVFGALLDARGHRPLLVVAPVQTDRTADSMAEIV
                     GEFRSILGDRPIAGEEFASLKRNMELRLPGRFETLASLEDAAHDLVNYGYSPAFYYDY
                     AKTVRALGEQDLAEAVNRVINPEQLIWVVVGDLEKIEAQVRALGFGEVVRLKASGASG
                     D"
     gene            2896..3954
                     /locus_tag="AC16UZ_12475"
     CDS             2896..3954
                     /locus_tag="AC16UZ_12475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6247544.1"
                     /translation="MADDYRERMQAYAQRLMGYKTGEMVSLMVHIGDELGLYRAMAGQ
                     DSVTAESLAEATGLHERWVAEWLRGQGAAEILDHVGDERFRLSDVAEQALLNEDSPAY
                     LQGFFSRPPSHEIVDRTIEAFSTGVGVSWGAHGDSASHFLCRTNRPFHMKLADEVIPL
                     LGGMSERLEAGGAVLDVGCGPGTALTELARAFPNSQFVGVDPAANMIAQAQQRYADMD
                     NVRFEVGYGETLEDVAAYDLVTTFDCMHDMTDPEGTMRAVRRAVKGDGAWLIKDIKAQ
                     DSYAENLENPVAAMMYGFSVLFCMSSALSKPGGAGLGTLGFPPSVCERMGHDAGFGQF
                     RILDFEGDPFNFYYELRP"
     gene            complement(3980..5071)
                     /locus_tag="AC16UZ_12480"
     CDS             complement(3980..5071)
                     /locus_tag="AC16UZ_12480"
                     /inference="COORDINATES: protein motif:HMM:NF014954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6247545.1"
                     /translation="MTNDELAVPETPADVTPAWLTRALGQRWPGIEVRSVVGEPLGEG
                     IGQISLLQRLTLEHAGAAEAPRHMVLKMHTTHADMLGVAVRFRMYEREANFYNRFADR
                     VPLRTPEVYHAATEGLERCVMLMEDMSSWHSPDQLAGPTLEEAELAVDSLATLTAAFW
                     NSPELSDHASWLPDFNVGYMSDTGNDYRQCLPEFLRRYETKLPPGSADAARTIADRYD
                     DLTRILSTGPQVLTHYDYRLENMFFRNDAAEAFCALDWQLVMRARPGWDIAYFMGTNL
                     PVSFLDEHGEALRDRYYQQLQSNGVQGYARADLEADLAIGTMAMTGIPIIGGANADLS
                     NPRNEALFTEVGSRAFGAVIGNDCLAYLP"
     gene            5235..5579
                     /locus_tag="AC16UZ_12485"
     CDS             5235..5579
                     /locus_tag="AC16UZ_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HIR6247546.1"
                     /translation="MINIEHDYFSDEATPLAEIEAAGYHAVPLDFPAEENENHWHDFN
                     SLLYITEGELTVTYAETGETCTCGPGTKITGVPNVVHREKTEGYKALIGIPVPPAELT
                     QPINKPLPAPAG"
     gene            complement(5580..6029)
                     /locus_tag="AC16UZ_12490"
     CDS             complement(5580..6029)
                     /locus_tag="AC16UZ_12490"
                     /inference="COORDINATES: protein motif:HMM:NF025559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HIR6247547.1"
                     /translation="MSETRLTNLERTRIEMAYAVPLIRDLQRLLGAEVINDALSRRFD
                     DQIDATAAPGEKADFSRMEAGTATFAEGGALDYEIIGSDAEHFDLNITGCRYAEMMDE
                     LGARDIGHLLICNRDYPAAAKIGMALTRTQTRMQGAGYCDFRYRRRG"
     gene            complement(6098..8116)
                     /locus_tag="AC16UZ_12495"
     CDS             complement(6098..8116)
                     /locus_tag="AC16UZ_12495"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR6247548.1"
                     /translation="MRFTFLFLSILLAATLAGNASAASYRSAPSEPVTNGFDPVQRAA
                     ERRALYDQLWVEKLVVTEPGVRVRLNDEDLRALSETVSQGNRQVPAERRQRIGVVKPV
                     AHGVDFAGMGAAPTRPKSFAGGQLLASGGGLTWTALVRAEGATASRLHFTGVNLPEGA
                     ELFVFNERGQAYGPYTGSGRNGNGAFWSEALIGTDTYVHLRVPGGLSAAALRGLRFVI
                     NDVGHIGPDYRYVDVWSTCEGNAGCVELAKGTTTHSAVADAKEAVAQMQFVSGPFIYY
                     CSGGLINDTDSGSATPPYFLTANHCLSKNNEANSLQTYFNFVDCGGNASQPPMATGAS
                     VLSSSRTSDYTLLLLDEVPTGTTLLGWNSTPVASSFGLDLYRISHPSGAPQAYSKHSV
                     STSRPECRSWPRGPWIYSDDVFGATEGGSSGSPVVNAAGQVVGQLSGACGYETSNVCS
                     DLNATVDGAFATYFNDVAQWLDPAPTSSGNNPPTASFRWSGDPDVMFTDTSTDLDEND
                     SISSWAWNFGDGLTSTATSPSHTYAYACNGTYNVTLTVEDTNGASDSASNAVTITADD
                     VCENLTIGVKDPLAGSIDESGRRPAVTFRINVATSDDGGSPNGAVVSGDWGGLVKGSG
                     SCTVNGSSCEVIKGNIRGSGDVTFTVTGIELSGYTFDGTEVTGTVQVP"
     gene            8344..9102
                     /locus_tag="AC16UZ_12500"
     CDS             8344..9102
                     /locus_tag="AC16UZ_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247549.1"
                     /translation="MKVSTIITAIAMLFVLSCTTVAQETGRISEAFACNFKDGKGSKD
                     LWAAVDFWNEQMDAMGAGPLDDYFAAILTPYRATTGADFFWLGSSSNLNSFAAGGHAY
                     VNNPQGQAADARFEEISRCDSNVFLSETLYDGLGAPDDVDPSAFLETFACTLNEDATM
                     DDVTAAEEGWIAHAKSAGAKVDVIRWTPLYANTSADVYYLVVSADMPSFGAYRTAWLT
                     SEGGQAADRAFDEITNCESGLMIGTRIRVPAPVD"
     gene            complement(9250..>9724)
                     /locus_tag="AC16UZ_12505"
     CDS             complement(9250..>9724)
                     /locus_tag="AC16UZ_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247550.1"
                     /translation="PIGLPAGAHDPDPEAALLDEIAAIQPWYELARERHGRTTVGASG
                     MDIAEVAIRLNGLLDPQGAGSFEEAGESRKQLHHLVGDLRAYYTEAATAQPGRTGLTP
                     SSREIENWFWEETEAAQMLLRLRDRLSDSADPELAFFGRLLTVPFSHQHLAPAGS"
BASE COUNT         1673 a         3011 c         3258 g         1782 t
ORIGIN      
        1 gcccggcgga agtggcgcag gccggatggc cggtgatcgc cgtgttctcg cgagccatgg
       61 cgacaacgag gtgtgactga tgcgcagtgt cttccggttc atgtgtttta tgttgtttgt
      121 ctcagcagga ccggcaagcc ttgcccgggg cgccgttgac gttgaccagc tggccatacc
      181 ccacgagacg ttcgtgctcg aaaacggtct caccgtgatc gtgcacgagg accacagcgt
      241 gcccatcgtc gccgtgaacc tctggtacca cgtgggatcc cgtaacgaaa gccggggccg
      301 gacgggcttt gcgcacctct tcgagcactt cttcttcaac ggcagcgagc actacccgca
      361 cgatttccgt cgcgccatgg acgacgtcgg cgccaacaac cgcaacggca cgaccaaccc
      421 ggaccgcacg aacttcttcg aggacgtgcc tgtctccgcg ctcgagcgca cgctctatct
      481 ggaagccgac cgcatgggct ttctcgccgc caacatcact caggagactc tggatcagga
      541 gcgcggcatc gttcagaacg aaaagcgtca gggcgaaaac cagccctatg gaaaggtctg
      601 gaaccgcatt gtggagtcga tctaccccta ttcgcacccc tacagctggc ccatcatcgg
      661 cagcatggcc gacctcgaag cggccacgct cgacgatgtc aaagcctggt acgccaagta
      721 ctacggtccg tccaactgcg tgctgtccct ggcgggcgac atcaccgtcg agcaggccag
      781 ggaactcgtt ggcaagtact tcgcagggat tcctccgggt cctccggtgg agcgttatcg
      841 tcagtgggtt cccactctgg agcggccgct gcgcgacgtg atgcacgacc gggttcctca
      901 ggcacgcatc tatcgcgtct atcacacgcc gggctggctc gacgccgaat ccgtgttgat
      961 cgagctgaac gcagggctgc tcagcggcag ccgcagcgcg ccgctggata gggccctcgt
     1021 ctacgacaaa caggtggcca cggaggtcgc cgccttcgcc tgggacaagg agatcgcggg
     1081 caacctcgtg gtggtggtct ccgtcaagcc cggcgtggac ccggccgatg cggagcggga
     1141 gctagatgcc gtgatggccc aattcctcgg tcagaagcct gcgcgaagtg ctctcgatcg
     1201 cgccaggacc cgacaggttg cgcagttcat ccgcggcatg gagcgtctcg gcggctttgg
     1261 cggccggtcg gacattctgg ccgaaagcat gacctacgcg ggcgatccgg aagccttcct
     1321 gcagcgctac cggaaagtga tggaggcaac gccggcggag ttgcacgcgg cttcgacgaa
     1381 atggctcgat gcgaatcact acacgctcac cgtgctgccg ttcccggagc tcaaagctgc
     1441 cgctgaagac gtggatcgca cctcgctgcc agcgctgggg ccggctccgg aggtcgcctt
     1501 tcccgagatc ggttcccgcg agctcgacaa cggtttgcgg gtgctgctcc tcgagcggcg
     1561 cggcgcgcct ctcgtgaaca tgtcgctggc cgtggacgcg gggtacgcgg ccgacccggc
     1621 cgccgcggcg ggcacggctt cgcttgcgct tcagctgctg gaagagggca cccgatcccg
     1681 tgatggcttt gcgctggccg acgatctcga cgcgcttgga gccaccatca gctcggccag
     1741 ctcccttgat ctatccttcg tgcagttgcg cgcgctcaaa gcgaatctcg cccgttcgct
     1801 cgccgtgttt gccgacgtgg ttcgcaatcc ggcatttgcc ggggacatgg tcgcgctggg
     1861 taagcgtcgc cggcttgcgc agatcgctca ggaggaagcg cagccgttct ccgcagctct
     1921 gcgaatggtg ccgccgcttc tgtacggaga tggccacgtc tacgccaagc cgcttacggg
     1981 atccggatat gtcgaaacgg tgcgcaacat cgagccggac gatctctcta aatggcactc
     2041 cgcctggttc aggcccaaca acgcgacgct gatcgtcact ggtgacgtga cctttgacga
     2101 cctcatgccc atggtggatg acgtgttagg agactggaag cccggcgccg ttccgctcaa
     2161 gcgcgtcgcg gacgtacccc gtggcaaagc aggtcgcatc tatctcgtcg atcggccgga
     2221 cgcgtcgcaa tcggtcatcg tggccgctca ggcgtccatt cccggcgggc aggcggagga
     2281 agtggctatc gaggcggcga tgcgggcctt cggcggcatg tcgacctccc ggttcaatca
     2341 gaacctcaga atcgacaagc actggagcta cggcgtgttc ggtgcgctgc tcgatgcgcg
     2401 cgggcaccgt ccgctgctcg tggtggcgcc ggtgcagacc gaccggaccg cggattccat
     2461 ggccgagatc gtcggtgagt ttcgttcgat tctgggagat cggcccattg ccggagagga
     2521 gtttgcgagc ctcaaacgaa acatggagct gaggttgccc gggcgtttcg aaacgctggc
     2581 gtcactggaa gacgcggcgc acgatctggt caactacggg tactcgccgg ccttctacta
     2641 cgactacgcc aagaccgtgc gggcgctcgg cgagcaggat ctggccgagg cggtgaaccg
     2701 ggtgattaac cctgagcagc tcatctgggt ggtagtcggc gacctggaga agatagaggc
     2761 gcaggtgcgc gcactagggt tcggcgaggt ggtcaggctc aaggcgtcgg gtgcttccgg
     2821 cgactgagcg ctggcttgct gcgggatgcg gcggcgaaac gccgcgggga tacaacggta
     2881 acggggaaac ttgccatggc ggacgattat cgggaacgga tgcaggccta cgcccagcgt
     2941 ctgatgggct acaagaccgg cgagatggtg tctctgatgg tgcacattgg cgatgagctc
     3001 ggcctttacc gggccatggc gggacaggac agcgtgacgg ctgaatccct ggccgaggcc
     3061 accggcctgc acgagcggtg ggttgccgaa tggctgcgcg gccagggcgc cgcggagatt
     3121 ctcgatcacg ttggcgacga gcggttcagg ctgtcggacg tggccgagca ggcgcttctg
     3181 aacgaggaca gccccgcgta tctgcagggc ttcttctccc ggccgccgtc ccacgagatc
     3241 gtcgaccgga ccatcgaggc tttcagcacg ggtgtggggg tcagctgggg cgcccacggc
     3301 gattcggcct cccattttct ctgccggacc aatcggcctt tccacatgaa gctcgcagac
     3361 gaggtgatcc ccctgctggg cggaatgtcg gaacgactcg aagccggtgg tgccgtcctg
     3421 gacgtgggtt gcggtcctgg cacggcgctg accgagctgg caagagcctt tcccaactcg
     3481 cagttcgtag gtgtcgatcc agcggccaac atgatcgctc aggctcagca gcgctatgcc
     3541 gacatggaca acgtgcgctt cgaggtgggc tacggcgaga cgctggagga cgtggcggcc
     3601 tacgacctcg tcaccacctt cgactgcatg cacgacatga cggatccgga gggaaccatg
     3661 cgggcggtgc gcagagctgt taagggagat ggcgcctggc tgatcaagga catcaaggca
     3721 caggacagct acgccgagaa tctggagaac ccggtggccg cgatgatgta cggtttctca
     3781 gtgctgtttt gcatgtcatc ggcgctctcg aagccgggcg gcgccgggct cggcaccctg
     3841 ggattcccgc ccagcgtctg cgagcgcatg ggccacgatg cgggcttcgg tcagttccgg
     3901 atcctggact tcgaaggcga tccgttcaat ttctactatg agctcagacc ctgacggcgt
     3961 tgcctgatcg gttcctgaat caaggcaggt aggccaggca gtcgttgccg atcaccgcgc
     4021 cgaaagcccg gctgccgacc tcggtgaaga gggcttcgtt gcgcgggttg gacaggtccg
     4081 cgttggcgcc gccgatgatg ggtatgcccg tcatggccat ggtgccgatt gcgaggtcgg
     4141 cctctaaatc ggcgcgcgca tacccctgga ccccgttgct ctgtagctgt tggtagtagc
     4201 ggtcgcgtaa agcctcgccg tgctcgtcca gaaaagacac gggcaggttc gtgcccatga
     4261 agtaggcgat gtcccaccca ggccgtgccc gcatcaccag ctgccagtcc agggcgcaga
     4321 aggcctctgc cgcgtcgttg cggaagaaca tgttttccag acggtagtcg tagtgcgtca
     4381 gcacctgggg gcccgtgctg aggattcgtg tcaggtcgtc ataccggtcg gctatggtgc
     4441 gtgctgcgtc ggcggagccg gggggcagct tcgtttcgta gcggcgcaga aactcgggta
     4501 ggcactggcg gtagtcgttg cccgtgtcgc tcatgtaacc cacgttgaaa tccggcagcc
     4561 agctggcatg gtccgacagc tcgggcgagt tccagaaggc tgccgtcagg gtcgccaggc
     4621 tgtccacggc cagctcggcc tcttccagag tggggccagc cagctggtcg ggtgagtgcc
     4681 agctgctcat gtcctccatc agcatgacgc agcgctcgag cccttccgta gccgcgtggt
     4741 agacctccgg agtccgaagc ggaacccggt ctgcaaatcg gttgtagaaa ttcgcctccc
     4801 gctcgtacat ccggaaacgc accgcaaccc ccagcatgtc ggcgtgggtg gtatgcatct
     4861 tgagcaccat gtgccggggc gcctccgcag ctccggcgtg ctcgagggtc aggcgttgca
     4921 gcaggctgat ctgaccgatg ccctctccga ggggttcgcc gaccacgctg cgcacctcga
     4981 tccccggcca gcgctggccg agcgccctgg tgagccaggc gggcgtgacg tctgcgggtg
     5041 tttcaggtac cgctaattca tcgttggtca tgcgtgtatc ctccctgcct cggagcctaa
     5101 atcggccgga gagcggagta aacggtggga caacccggac agggcgcgtg acacctgatc
     5161 gaccggtgcg atcatgcaag agtggacaga ggccggaatg tgggtgtacg ttcgatccgg
     5221 atgaggggag caacatgatc aacatcgagc acgactactt ttccgacgag gcgacgcccc
     5281 tggcggagat cgaggcggcc ggctaccacg ccgttcccct ggacttcccc gccgaggaga
     5341 acgagaacca ctggcacgat ttcaactcgt tgctgtacat caccgagggc gagctgacgg
     5401 tcacttacgc cgagaccggc gagacctgca cgtgcgggcc cggaaccaag atcaccggtg
     5461 tcccgaacgt cgtgcaccgg gagaagacgg aaggctacaa agcgctgatc ggcattcccg
     5521 tgccgccggc agagcttacc cagcccatca acaagccgct gcccgcaccg gccggctgat
     5581 cagccccggc gccggtagcg gaagtcgcag tagccggcgc cctgcatgcg cgtctgggtg
     5641 cgggtaaggg ccatgccgat cttcgcggcg gcggggtagt cgcggttgca gatgagcagg
     5701 tggccgatgt cccgggcacc gagctcgtcc atcatctccg cgtatcggca gccggtgatg
     5761 ttcaggtcga aatgctcggc gtcggagccg atgatctcat aatccagcgc gccgccctcg
     5821 gcgaacgtcg cggtaccggc ctccatccgg gagaagtcgg ccttctcgcc cggtgcggcc
     5881 gtcgcatcga tctgatcgtc gaaccggcgt gaaagcgcat cgttgatcac ttccgcgccg
     5941 agcagccgct gcagatcgcg gatcagcggc accgcgtagg ccatctcgat gcgcgtacgt
     6001 tccaggttgg tgagtctggt ctcgctcatg ggtggttccc ccattctgtg cggaaggcgc
     6061 ggtttgcggc cgcgcctgct ccatgactac ccgaggctca ggggacctga accgtgccgg
     6121 tgacttctgt gccgtcgaag gtgtatccgg agagctcgat gcccgtgacg gtaaaggtga
     6181 cgtcaccgct gccgcggatg ttgcccttga tcacctcgca ggacgaaccg ttcacggtgc
     6241 aggagccgga ccctttgacc agtccgcccc aatcaccgga taccacagcg ccgttcgggg
     6301 agccgccatc atcgctggtg gccacattga tcctaaaggt caccgccggc cggcggccgc
     6361 tttcatcaat gctgcccgcc agggggtctt ttacgcctat cgtcagattt tcgcagacgt
     6421 cgtcggcagt gatcgtgacg gcattcgacg cggagtcgct tgcgccgttg gtatcttcaa
     6481 ccgtgagcgt gacgttgtac gtcccgttac aggcgtaggc gtaggtgtgg ctcgggctcg
     6541 ttgccgtgct ggttaggccg tcgccgaaat tccaggccca gctggagatg ctgtcgtttt
     6601 catcgaggtc ggtgcttgtg tcggtgaaca tgacgtccgg atcgccggac catctgaagc
     6661 tcgccgtggg cgggttgttg ccactgctcg tcggtgccgg atcgagccat tgagcgacgt
     6721 cgttgaagta cgtggcgaac gcaccgtcca cggtagcgtt gaggtcgctg cagacgttgg
     6781 aggtttcgta gccgcaagcg ccggacaatt ggccgacgac ttggcctgcc gcgtttacga
     6841 ccggagagcc gctgctgccg ccctcggtgg cgccgaagac gtcgtcgctg tagatccacg
     6901 gcccgcgtgg ccagctgcgg cattcaggac ggctggtgct gactgaatgt ttcgagtacg
     6961 cctgaggcgc gcctgacggg tggctgatac ggtaaagatc caagccgaaa gaactggcga
     7021 ctggagtgct gttccagccc agcagggtcg tgccggtcgg gacctcgtcc aggagcagca
     7081 gcgtgtaatc gctcgtacgg ctggagctca agacgctcgc gcctgtggcc atgggcggct
     7141 gggaggcgtt gccgccgcaa tcaacgaaat tgaagtaggt ctgaagcgag ttcgcctcgt
     7201 tgttcttgct caggcaatga ttggcggtca gaaaataggg cggtgtggcg ctgccgctgt
     7261 cggtgtcgtt gatcaggccg ccgctgcagt agtagatgaa cgggccggaa acgaactgca
     7321 tttgggcgac ggcctctttc gcgtcggcta cggcggaatg ggtcgtcgtg cctttggcaa
     7381 gctcaacgca gccggcgttc ccctcacagg tgctccagac gtcaacgtac cggtagtccg
     7441 gcccgatgtg accgacgtcg ttgatgacga agcgcaggcc tcggagtgcg gccgcgctca
     7501 agccgcccgg cacgcgcagg tgcacgtagg tgtcggtgcc gatgagcgct tcggaccaga
     7561 acgcgccgtt gccgttgcgg cctgagcccg tatagggccc gtaggcctgg ccgcgctcgt
     7621 tgaagacgaa gagctcggcg ccttctggca gattgacccc ggtgaagtgc agccggctcg
     7681 cggtggcgcc ctcggctcgc acgagcgccg tccaagtgag gccgccgccg ctggccagga
     7741 gctggccgcc ggcaaacgac tttggccgcg tgggcgcggc acccatgccg gcgaagtcca
     7801 cgccgtgggc gacgggcttg accacgccga tgcgctggcg tcgctctgcc gggacctggc
     7861 gattgccctg ggacaccgtc tcgctcaaag cccggagatc ctcgtcgttg agccgaaccc
     7921 ggacccccgg ctccgtcacc acgagctttt cgacccagag ctggtcgtag agcgcgcgcc
     7981 tctcagccgc acgctgaacg gggtcgaaac cgttggtcac gggttcggag ggtgctgatc
     8041 ggtagctcgc ggcgctggcg ttgccggcaa gggtggcggc gagcaggata ctcagaaaca
     8101 ggaaagtaaa acgcatacct gacaaaactg atgattgcaa tgatccaaat gtagcagacc
     8161 tcgccacgag ttggggcatc gttttgtgac gatttgcaag gtttcgaggc tgcgaatctg
     8221 cagcgtatca tcaccagggg aagagagacg tcgaccggcg gtccggaagc ggacggcctt
     8281 tgggttagac gcgctgttcc cgcatgagtg acgaaagatg aacgggacaa atggggggaa
     8341 ccaatgaaag tctcaacgat catcaccgcg atcgccatgc tgttcgtgct gtcctgcacg
     8401 acggtggccc aggagacggg ccgcatcagc gaggccttcg cgtgtaattt caaggacggc
     8461 aaaggcagca aggacctgtg ggccgccgtg gacttctgga acgagcagat ggatgccatg
     8521 ggagctggcc cgctggacga ctacttcgcg gcgatcctga cgccgtaccg ggcaaccacg
     8581 ggggctgatt tcttctggct cggatcctcg tcaaatctca attcgttcgc cgccgggggg
     8641 catgcctacg tcaacaaccc ccagggtcag gcggccgacg cgcgcttcga ggagatttcg
     8701 cggtgcgatt ccaacgtctt cttgtctgag actctctacg acggcctcgg cgcgccggac
     8761 gacgtggatc cttccgcgtt tctggagacc ttcgcgtgca ccctgaatga agacgctacc
     8821 atggacgacg tgacggccgc cgaggagggc tggattgctc acgccaaatc cgcaggtgcg
     8881 aaagtcgacg tgatccgctg gacgccgctc tacgccaaca cgtccgcgga cgtctactac
     8941 ctggtggtca gcgcggacat gccgtctttc ggcgcgtacc gcacggcctg gttgacgtct
     9001 gaaggtggcc aggctgccga tcgggcgttc gatgagatca ccaactgcga atccgggttg
     9061 atgatcggca cgcgcatccg cgtgccggcg ccggtcgatt aacgccggcg ggtttcgatt
     9121 ctcctcgacg aaaggcgggc cgcggcctcg gcggcccgcg aactaggggg aaagcccgat
     9181 agggtcccgc tgcggatcca gcaccatcat gactggctgg gtcacatcat cggcaatacc
     9241 tgagcctgct cagctgccgg ccggcgccag atgctggtgg ctgaagggta cggtcagcag
     9301 ccggccgaaa aaggcgagct ccgggtcggc gctgtcggac agccgatcgc gcaggcgaag
     9361 aagcatctga gccgcctcgg tctcctccca gaaccagttc tcgatctctc tgctcgatgg
     9421 ggtcaggccg gtgcggcccg gctgtgccgt ggcggcttcg gtgtagtacg ccctcagatc
     9481 gccgacgaga tgatggagct gcttcctgct ctctccggcc tcttcgaaag agcccgcgcc
     9541 ttgcgggtcg agtaggccat tcagacgtat ggcaacctcg gcgatgtcca tcccgctggc
     9601 tccgaccgtg gtccgcccgt ggcgttcgcg cgcgagctcg taccagggct ggatcgcggc
     9661 gatctcgtcc agcagagccg cctcgggatc ggggtcgtgc gctccggcag gcaggcctat
     9721 gggg
//
LOCUS       DBNHOX010000567         1517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1010497, whole genome shotgun sequence.
ACCESSION   DBNHOX010000567 DBNHOX010000000
VERSION     DBNHOX010000567.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1010497"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..370
                     /locus_tag="AC16UZ_12510"
     CDS             209..370
                     /locus_tag="AC16UZ_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247551.1"
                     /translation="MSEQPKTRPKVPEGKARFVTTRQKEPNEKGFVGYETTWEPFQKE
                     VPYTIPKKP"
     gene            545..>1517
                     /locus_tag="AC16UZ_12515"
     CDS             545..>1517
                     /locus_tag="AC16UZ_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR6247552.1"
                     /translation="MRSPSRSLLGTGGLLVAGLSLLVTSGVAPRAVSGSDLVVPLDPP
                     RGLYTIDGTVRFESGAAVVSGVEEIVLTNNSPQPLTRLAFDWSPGPQHAMEVSVGGIP
                     ARPLSHGADAEESLVLVTLPESIGSGQELRVSVRFESTYTIEPKENKHLWTSWFPRID
                     WGVRTQDDFVLRIDYPSEYTFASSARHDPASDAWRARGVERFGLFLMRDAEVLEQQVG
                     DVGVRVVHTPDGLDAARLIMETAVDVVRFYRDRFGFYPSDHIDIIPGEEEPMGGYPAA
                     TSLVVIHGMERMSERPELHWRWITAHELGHQYWLEHVMSDSRQQWGWL"
BASE COUNT          290 a          465 c          481 g          281 t
ORIGIN      
        1 atcaccatcg atggcaacaa cccgctgtgt ggccgcgagg tcattttcaa gctgaaaata
       61 ctgctggtcc gcgctgccag cgaagaggag attgcagccg gcgggaaagt cgaagcaggc
      121 ccggatattc aaacagggcc ccgtatccac gaggttttga actaaactga cgtcagctgc
      181 gaatttgtct tagcgagagg taagcatcat gagtgaacaa cccaaaacca ggcccaaagt
      241 gcctgaaggc aaggcccgtt tcgtgacaac ccgtcagaaa gaacccaacg agaaagggtt
      301 tgtcgggtat gaaaccacct gggagccgtt ccagaaagag gttccgtaca ccatcccgaa
      361 gaagccgtaa gcttcccgga cgcgcgaggc ccgcccagtt gccgactaac cgggagttca
      421 ggttgcagtg cacacgcccg gtatgtggat ttctcgccca actttaatca gccatacttg
      481 ctggaaccag gcaactcgtg gtttattctt cctaaccttc gcgacaacgt tcgaggatat
      541 gcccatgcgc tcgccatcac gctcgctgct tggcaccggc ggtttgctcg tcgccggttt
      601 gtcccttctc gtgaccagtg gtgtggcgcc gcgggccgtt tccggtagcg acctcgtcgt
      661 gcctctcgat ccacctcgcg gcctgtacac catcgacggt accgttcgct tcgagtccgg
      721 agcggcggtg gtctcggggg tggaggagat cgtcctgacc aacaactcgc cgcagccgct
      781 cacccggctc gccttcgatt ggagccctgg gccccaacac gcgatggagg tgagcgtcgg
      841 tggcatcccg gcgcggccgc tctcgcacgg cgcagatgcc gaggagtctt tggtgctggt
      901 gactttgccg gagagcatcg ggagcgggca ggagctcagg gtgagcgtcc gcttcgagag
      961 cacctacacc atcgagccga aagagaacaa acacctgtgg acgtcatggt tccctcgcat
     1021 cgactggggc gtgcggaccc aggacgactt cgtcctcagg atcgactacc cctccgagta
     1081 cacctttgcc tccagcgccc gccacgaccc ggcgagcgat gcctggcgtg cgcgcggggt
     1141 cgagaggttc ggcctgtttc tcatgagaga cgcagaggtg ctggagcagc aggtcggcga
     1201 tgtcggcgtg cgggtggtgc acaccccgga cgggctcgac gccgcccggc tgatcatgga
     1261 gaccgccgtc gatgtggtgc ggttctatcg cgaccgcttc gggttctacc cctcggacca
     1321 cattgacatc atccccggtg aagaggagcc gatggggggg taccctgccg cgaccagtct
     1381 cgtggtcatc cacggcatgg agcggatgtc cgagaggccg gagctgcact ggcggtggat
     1441 cacggcacac gagctcggtc accagtactg gctcgagcac gtcatgtcgg actcgcggca
     1501 gcagtggggc tggctga
//
LOCUS       DBNHOX010000568         2381 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1266396, whole genome shotgun sequence.
ACCESSION   DBNHOX010000568 DBNHOX010000000
VERSION     DBNHOX010000568.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1266396"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..496)
                     /locus_tag="AC16UZ_12520"
     CDS             complement(35..496)
                     /locus_tag="AC16UZ_12520"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6247553.1"
                     /translation="MSTEDNRRLIERWYEALAAGDFQTIFDMHHDDVVYNMVGNTPVS
                     GRIYGKDACCNGMIAQKLLDKLVPDEVRFAKRWKIVAAEGDRVVGLMQGGGPTRNGQS
                     YEQTYCEIFTIRDGKIVEMHTFFDTVLVEQCLFDNPLERPEAPLADPFQVG"
     gene            complement(541..>2381)
                     /locus_tag="AC16UZ_12525"
     CDS             complement(541..>2381)
                     /locus_tag="AC16UZ_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6247554.1"
                     /translation="DRNVDDGDGDTVWIGGSWGFDIGERGFMRFSGDYRDQDATNRAG
                     YDPRELYPRQPDGSLDPREETASRLNHRHGQAQVEQFNLVINAAIPVSERMALYGLAT
                     IGSLRSDTAADFRRPVDVRNVLEIYPNGFLPEIRSDIDDRAFVVGLTGEHFGWSWDMA
                     LNTGENEIDWAAEDTLNTSMGALSPTKFSTGNNEYHLRTFGIEANRPFGENFHVRVGA
                     EMLAEEYEVEKGETASTIDGGFTDVNGDPAEVGSQGFPGFKRRELEQDRDVYSLFAKV
                     DANLGERTRLSATARYDDYDDVGGIFGGRLAGSFQLSPKIALRASAAMGAKAPELSQG
                     NYEKTVTVFDTGSAAEAGYFPADSDVALALGGSELSEEEFVSFDAGVTLELVPSLMLS
                     ADFYRIEVDDAVVLTDNLAGDALVGVLGDGGVNDVARAAFFLNGVDTRAQGVNLSARY
                     PLETRYGDLTFSLDANFNDNKVSNVDEEPADLVAAGLARLPDTVATRLEDGRPESRTN
                     LTVDWQRKRLGMRMDLLHYGKTTDATAGFKLDSDIVLNLSARYQATPRVALDFGAHNI
                     LDAYPDPYDLEADDATDFDRIYPFSRYSPYGINGRLVYLRASVRFD"
BASE COUNT          367 a          775 c          790 g          449 t
ORIGIN      
        1 tggagtgaca agtcggtcat aacagttcct ttggctatcc aacctggaag ggatcggcca
       61 gcggtgcctc cggccgctcg agaggattgt caaacaggca ttgctctacc agcaccgtgt
      121 cgaaaaaggt gtgcatctcg acgatcttac cgtcgcggat ggtgaagatc tcgcagtagg
      181 tctgctcgta gctctgaccg ttcctggtcg gcccgccgcc ctgcatcagg cccaccaccc
      241 gatcgccttc ggccgccacg atcttccacc gcttggcgaa gcggacctcg tccgggacga
      301 gcttgtcgag cagcttctgg gcaatcatgc cgttgcagca ggcatccttg ccgtagatgc
      361 gtccggacac gggcgtgttg cccaccatgt tgtacaccac gtcgtcgtgg tgcatgtcga
      421 agatggtctg gaagtctccg gcagccaggg cttcgtacca gcgctcgatc agccggcggt
      481 tgtcttcggt gctcatgggc ctctcctcgt cgcctggtgc ggcattttag ccggtgagcg
      541 tcagtcgaag cgcacgctgg cccgcagata gacgagcctg ccgttgatgc cgtagggcga
      601 gtaccgcgag aaggggtaga tgcgatcgaa gtcggtggca tcgtccgctt ccagatcata
      661 gggatcggga taggcatcca ggatgttgtg ggcgccgaag tccagcgcca cccggggcgt
      721 tgcctgatac cgggccgaga ggttcagaac gatgtcggag tcgagcttga agccggcggt
      781 ggcgtccgtg gtcttgccgt agtgcagcag atccatgcgc atgcccaggc gtttgcgctg
      841 ccagtccacg gtcaggttgg tccggctctc cggccgcccg tcctccaggc gcgtggccac
      901 cgtatccggc agccttgcga gtccggccgc gaccagatcg gcgggctcct cgtccacgtt
      961 gctgaccttg ttatcgttga agttcgcatc gagcgagaag gtgaggtcgc cgtagcgtgt
     1021 ttccagcggg tagcgggcgg acaggttgac gccctgggca cgggtgtcca caccgttcaa
     1081 gaagaaggcg gcccgggcca cgtcgttcac gccgccgtcg cccagaacgc ccaccagggc
     1141 atcgcccgcc aggttgtccg tgagcaccac ggcgtcgtcc acctcgatgc ggtagaaatc
     1201 ggcgctgagc atcagcgacg gcacgagctc cagggtgacc cccgcatcga agcttacgaa
     1261 ctcttcttcg ctgagctcgc tgccgcccag cgcgagtgcc acgtccgagt cggccggaaa
     1321 gtacccggcc tcggcggcgg atcccgtatc gaacaccgtc accgtcttct cgtagttgcc
     1381 ctgggagagc tccggcgctt tggcgcccat ggcggcggat gcccgcagcg cgatcttcgg
     1441 ggagagctgg aagctgcccg ccaggcggcc gccgaagatg ccgcccacgt cgtcgtagtc
     1501 gtcgtagcgc gcggtggcgg acagccgggt gcgttcgccc aggttggcgt ccacctttgc
     1561 gaagaggctg tagacgtccc ggtcctgctc cagctcccgg cgcttgaacc cggggaagcc
     1621 ctgggagccc acctcggccg gatcgccgtt cacgtccgtg aagccgccgt cgatggtgga
     1681 ggcggtctcg cccttctcca cctcgtactc ctcggcgagc atctccgcgc cgaccctcac
     1741 gtggaagttc tccccgaagg gtctgtttgc ctcgatgccg aaggtgcgca ggtgatactc
     1801 gttgttgccg gtggagaact tggtggggct gagcgcgccc atcgaggtgt tcagcgtatc
     1861 ctccgccgcc cagtcgatct cgttctcgcc ggtgttcaat gccatgtccc aggaccagcc
     1921 gaagtgctcg cccgtaagcc ccaccacgaa ggcccggtcg tcgatgtccg agcgtatctc
     1981 ggggaggaag ccgttgggat agatctccag gacgttgcgg acgtccaccg gtcggcggaa
     2041 gtccgcggcg gtgtcgctgc gcagcgagcc gatggtggcc aggccataga gcgccatccg
     2101 ctcggagacg ggtatggccg cgttgatcac cagattgaac tgctccacct gggcctggcc
     2161 gtggcggtgg ttgagccggc tcgcggtctc ctcgcgggga tccaatgatc cgtccggctg
     2221 gcgcgggtaa agctcccggg ggtcgtagcc ggcccggttg gtggcgtcct ggtcccgata
     2281 gtcgccgctg aaacgcatga acccgcgctc gccgatgtcg aagccccagc tgccgccgat
     2341 ccagaccgtg tcgccgtcgc cgtcgtccac gttgcggtca c
//
LOCUS       DBNHOX010000569         2311 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_431400, whole genome shotgun sequence.
ACCESSION   DBNHOX010000569 DBNHOX010000000
VERSION     DBNHOX010000569.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_431400"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="AC16UZ_12530"
     CDS             complement(<1..817)
                     /locus_tag="AC16UZ_12530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR6247555.1"
                     /translation="MLGDATAEDVFTQDPYIVRKQPLSVLCVPIVHRNEITGILYVEH
                     RWLTGVFTAQRVEVLALLASQAAISIENARLYADLHDTQEQYRALYDNAIEGLFRIDP
                     DGALLSANPTLARILGFDDVKQLLAEYRDLIDRVFLSREDASHFLTNLEEHGLLNVFE
                     AQGVTRNGRTFWMALTARLSHDPEQGAYIDGSLVDISERMKREQADKHRQIAEAATQA
                     KSEFLANMSHEIRTPMNGVMGLAELLSATRLDDRQRQLLNGIQRSGKALLQIIN"
     gene            complement(824..>2311)
                     /locus_tag="AC16UZ_12535"
     CDS             complement(824..>2311)
                     /locus_tag="AC16UZ_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247556.1"
                     /translation="LHAGYHLGSEGLDALTDDMLSEAARHGYSAEIAFAFAARAVSAV
                     ADHRIGIAQRYAGEARALAGQFSGDAFSIRAETLLNGLVDPWSGGLDDTLGGLSRNID
                     ASMACQDYEFAATATAFYATNGLLRGLDLASLRRELNEQISRLSGLQHVTGVNISLFV
                     QQIIGSLLGHAEADGESDHFRELSISNSEDAVAHGYVYVLRLYYAVLFDDFRGAANIL
                     ELAEQFGPALAGSPLLIVWKFADALVNLRSGNTAGLRRARASLRELKQWHQDGADHAE
                     PKVILLQAELNWQRGATTQAIECYEVAADKARRLGLANDEALAYELAARACESNGRAD
                     FAKLFARNAHQAYLRWGANAKASHLEREFPSMLDDALHPATVIGDLTDLTVRDFSSMS
                     IRSSLDGSEINANVLDTTTVLRAAQTISSEIVLDRVLTKLLRLALEHAGAQKACMLLA
                     HDGRLFVEAIASVDGGPTRRESPATPLEASDDVPESIVHFVARTK"
BASE COUNT          360 a          722 c          811 g          418 t
ORIGIN      
        1 cgttgatgat ctggagaagc gccttccccg aacgctgaat gccgttcagc agctggcgct
       61 gccgatcatc cagcctggtg gcggagagga gctccgcgag gcccatgacg ccgttcatcg
      121 gcgtccggat ctcgtgactc atgttggcca ggaactcgct tttcgcctgg gttgcggcct
      181 cggcgatctg ccggtgcttg tcggcctgct cccgcttcat gcgctcggag atgtcgacga
      241 gagagccgtc gatgtaggcg ccctgctcgg ggtcgtggct caggcgcgcc gttagcgcca
      301 tccagaacgt gcggccgttg cgcgtgactc cctgggcctc gaagacgttg agcaggccgt
      361 gctcttcgag gttggtgagg aagtggctgg cgtcttcccg gctcaggaag acgcggtcga
      421 tgagatcacg gtactcggcc agcagctgct tgacgtcgtc gaagccgaga attcttgcca
      481 gcgtcgggtt ggcgctcagc agcgcgccgt cggggtcgat ccggaagagc ccttcgatgg
      541 cgttgtcgta cagcgctcgg tactgctcct gagtgtcgtg caggtcggcg tacagccgcg
      601 cgttctcgat cgagatcgcc gcctgagagg cgagcagggc gaggacttcc acccgctggg
      661 cagtgaaaac gccggtgagc cagcggtgct cgacgtagag gattccggtg atctcgttgc
      721 ggtgcacgat gggcacgcag aggacggaca gcggctgttt gcgaacgatg tatggatcct
      781 gggtaaagac gtcctccgcg gtggcgtcgc caagcaccag cgcctacttg gtgcgcgcca
      841 cgaagtgaac gatgctctcg ggcacgtcgt cggaggcctc cagcggggtc gccgggctct
      901 cgcggcgggt cgggccgccg tccaccgagg cgatggcttc gacgaacagc cttccgtcgt
      961 gggcgagcag catgcaggct ttctgggcgc cggcatgctc cagcgccagg cgcagcagct
     1021 tcgtcaggac gcggtcgagg acgatctcgc tggagatggt ttgagccgcg cgcagcaccg
     1081 tggtggtgtc caggacgttc gcgttgatct cgcttccatc caggctcgac cggatggaca
     1141 tggagctgaa gtcgcgcacg gtgaggtcgg tcaggtctcc gatcacggtc gcaggatgca
     1201 gcgcatcgtc gagcatgctc gggaattccc gctcgaggtg actggctttc gcatttgcgc
     1261 cccagcgcag gtaggcctga tgagcgttgc gcgcgaagag cttggcgaag tcggcccgac
     1321 cgttggactc gcaggctctg gccgccagct cgtaggccag cgcctcgtcg ttggcaaggc
     1381 cgagccgacg cgctttgtcg gcggcgacct cgtagcactc gatggcctgg gtggtggcgc
     1441 cgcgctgcca gttcagctcg gcctgcagca ggatgacctt cggctccgcg tggtcggcgc
     1501 cgtcctggtg ccactgtttc agctcacgca gcgacgcgcg tgcccttcgt aggccagccg
     1561 tgttgcccga gcgcaggttc accagagcgt cggcgaattt ccagacgatg agcagaggcg
     1621 accccgccag cgcggggccg aactgctcgg cgagctcgag aatgttggcc gcccccctga
     1681 aatcgtcgaa gagcacggcg tagtagaggc gcagcacgta cacgtagccg tgggcgaccg
     1741 cgtcctcgct gttggaaatg ctcagctccc tgaagtggtc cgactcgccg tcggcttccg
     1801 catgacccag caggctgccg atgatctgct gaacgaacag cgagatgttg acgcccgtga
     1861 cgtgctgaag accggaaagt ctggaaatct gctcgttcag ctctcgccgt agggacgcca
     1921 gatcgagccc gcgaagcagg ccgttggttg cgtagaaggc cgtggccgtg gcagcaaact
     1981 cgtagtcctg gcaggccatg ctggcgtcga tgttgcgcga aagcccgcca agcgtgtcgt
     2041 ccagcccccc cgaccatggg tccaccaggc cgttcagcag ggtttccgcg cggatggaga
     2101 aggcgtcccc tgagaactgg ccggcaaggg ctcgcgcctc cccggcgtag cgctgcgcga
     2161 tcccgatccg gtggtcggcg acggcgctga cggcacgcgc ggcgaacgca aaggcgatct
     2221 cggcgctgta gccgtgccgt gccgcctcgc tgagcatgtc gtctgtaagg gcgtccaggc
     2281 cctcgctgcc caggtggtat ccggcgtgca a
//
LOCUS       DBNHOX010000570         5420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1010618, whole genome shotgun sequence.
ACCESSION   DBNHOX010000570 DBNHOX010000000
VERSION     DBNHOX010000570.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1010618"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1957)
                     /locus_tag="AC16UZ_12540"
     CDS             complement(<1..1957)
                     /locus_tag="AC16UZ_12540"
                     /inference="COORDINATES: protein motif:HMM:NF013117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HIR6247557.1"
                     /translation="MRRSLGRHRLIMASCFIWLASWPGHASAQQTTSPDAAGSQGTPA
                     EVQPTTDLTSRTQATLTSLDRISGEINELGQLADDAAELDALQIQTDELISITENIDG
                     RGADASFDGSLVKARALAVRAEAVTTALSDHALAMEAKLEEIRQTTAQWQRIQGEITD
                     IPEAISQRIARILATGAGLENRINAKLNETVELQNEALGVLDVARSLQERFDSYGRSR
                     RGQLLEANAAPVWLMKSEHLSSSSEKAGRDFVGTLRRDYATWVETSQAIIGGHFLLLP
                     LLLFLLTKLKATALQSPTGALARPTSTGLLIWMLLGVAIYAGAPVAVRTVFVAIASIV
                     TGFVLVGFLDKRLRAGAIAFLILNVVEDLISGFRVTDALPRLVYLLLALGAIVLVMLA
                     RRPHTLSAMLAWRMPRPILVSGMWTGIALLVLSCIANVAGYVYLSQVLVNGVLASTGV
                     LLVLFAGLTSVSEIIVAVLSLDALNNVRSIANHRYRLNRALRRPLVVLAMFLWAWATT
                     RAFGIDDWVIASIGAIVGAEMSIGSVTLSLQGVLVFVIAVLSAVWASRIVRAVLNYDV
                     LPRMQLPRGVPNTISMTLHYSIILIGLLLGVGFMGIDLSSLAFIVGALGVGIGFGLQN
                     LVNNFVSGLILIFEAPIQVGDTVEVGPL"
     gene            complement(2102..3445)
                     /locus_tag="AC16UZ_12545"
     CDS             complement(2102..3445)
                     /locus_tag="AC16UZ_12545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR6247558.1"
                     /translation="MDLNTLTSFEQRYLWEPDYRQAPPWQRVLVGMARTLMLLFRDIF
                     HGDISLRAMSLVYTTLLSMVPLLALSFSILKGFGVQNQLDETLRAALAPLGEEKALEL
                     TENLITFVNNMNVGVLGAVGLLFLLYTVVSLMQKIEDSFNHTWRIRTPRTFGERFTTF
                     LSAITIGPLLIFTAIALTGSIMSTTVMTHVQEIAVVGPAIQIVVRLLPFVFVIGAFTF
                     LYVFIPNTRVRISAALTGGIVAGVLWELLGFGFASYASGASRYQLIYATFATAIFFLI
                     WLYLNWLILLIGASIAFYRQHPEVIASGSKEVHFSQQLTTRYVLSLLAKIGRRHYEKK
                     KPYTLDELAASYRLPTNALEHCLTLLVALDVLAETDDPKPCYIPALPLDTTTVAEVLQ
                     RVETYQPPGTYAPALGADEDIVTIEAAGGSGRDRALGGLTLKQLAAGDSSEVVTA"
     gene            3616..4494
                     /locus_tag="AC16UZ_12550"
     CDS             3616..4494
                     /locus_tag="AC16UZ_12550"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6247559.1"
                     /translation="MSDAQARGLPPDDPTTLITPELIEQYRRDGVICVRNALHPEWLM
                     LLEMGLERIMADPGQQKHLFYRGEPGEFIETIRNFDVAPEIRRLIYDSPIADMIGKLI
                     GSGNIWLYSDEFFVKEGGGCERTPWHQDLPYWPLEGEQIASMWISLDPLPREDCLEYV
                     AGSHQGPMFDGFDPQRVTEDPTLPHYGEGLPPLPDIEADRGRFNIVSWDISPGDVILA
                     HPGVLHGGGATGIAGRRRAITIRCYGDDVVYATRPPTRPTVPLTPGLGLKLKPGDPLR
                     HPYYPRLRPLPEHQRV"
     gene            complement(4564..>5420)
                     /locus_tag="AC16UZ_12555"
     CDS             complement(4564..>5420)
                     /locus_tag="AC16UZ_12555"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6247560.1"
                     /translation="IDAGEVVGPRLVPCSRNYMPAMSADSAMENEETCMAQGPEGFRK
                     GVLFDLERGVKIVKIFASGGHGAPAGMCMSAEEIGAVVEVARDHGARVRAHVAGKQQI
                     LDCLRAGVTIIDHGDEADEEVIEAYLEHDAFVLPSGYVLLKAAELGGNTFGFSEDLKD
                     FESLCRLLPRLVDAGVKLVPGDDFGIWQIPHGAYAEELVCYAEHAGIPPLELIKWSTL
                     HGGEMTGFEGIGTIAPGKVADLLILNDDPSRDIRVLTRPESVQAVLKGGQLVSGELPT
                     PLAAVSAG"
BASE COUNT          999 a         1694 c         1776 g          951 t
ORIGIN      
        1 tcagcggacc gacttccacc gtatcgccca cctgtatggg ggcttcgaag atcaggatca
       61 ggcccgagac gaagttgttg accaggttct gcaggccgaa gccgataccc acgccgagcg
      121 cgcccacgat gaatgccagg ctgctgaggt cgatgcccat gaagcccacg ccgagcagca
      181 ggccgatcag gatgatcgag tagtgcagcg tcatcgatat ggtgttggga acgccgcggg
      241 gcagctgcat tcgggggaga acgtcatagt tgagaacggc ccggacgatc cgcgaagccc
      301 agacggcgga cagcaccgcg atgacgaaga ccagcacccc ctgcaatgac agcgtaaccg
      361 agccgatcga catctcggcg ccgacgatcg cgccgatgga ggcgatgacc cagtcatcga
      421 tgccgaacgc gcgggtcgtt gcccatgccc acaggaacat ggccagcaca acgagcggtc
      481 tgcgcaacgc ccggttgagg cgatagcggt gattggcgat tgaccgtacg ttgttcagtg
      541 cgtccaggct cagcacggca acgatgatct cggagaccga cgtgagcccc gcgaacagca
      601 ccagcagcac cccggtgctg gccaggacgc cgttgaccag gacctgcgac aagtacacgt
      661 acccggcgac gttggcgatg cagctgagca ccagaagggc gatgcccgtc cacatcccgc
      721 tgaccaggat cggacggggc atgcgccagg cgagcatggc gctcaacgta tggggtcgtc
      781 gggccagcat cacgagaacg atggcgccca aggcaaggag caggtagacg agacgtggca
      841 gcgcgtcggt cacgcggaag ccgctgatca gatcctccac cacgttcagt atcaggaatg
      901 ctatcgcccc ggctcgcagt cttttgtcga ggaaaccgac gaggacgaac ccggtcacga
      961 tcgaggcaat cgccacgaag acggtacgca cggcgactgg cgccccggcg tagatcgcca
     1021 cgcccaaaag catccagatg aggagcccgg tcgacgtggg gcgggccaga gcgcccgtcg
     1081 gggattgcag tgccgtcgcc ttgagcttcg taagcaggaa cagcagcagt ggaagcagga
     1141 ggaagtgacc gccgatgatc gcctgggagg tttctaccca ggttgcgtag tcgcgacgca
     1201 gcgtgccgac gaagtcccga cccgctttct ccgaagagct gctgagatgt tcggatttca
     1261 tcagccagac cggggccgca ttcgcttcca gcagctgccc gcgccgggac cgcccgtagc
     1321 tgtcgaagcg ttcctgcagc gaccgggcga cgtcgagcac cccgagcgcc tcgttctgga
     1381 gctccacggt ttcgttcagc ttggcgttta tccggttctc caggcctgca ccggtcgcca
     1441 ggatcctcgc gatacgctgg ctgatggctt ccggaatgtc cgtgatctcg ccctgtatcc
     1501 gctgccactg ggccgtcgtc tggcggatct cttcgagttt ggcttccatg gcgagagcgt
     1561 ggtccgacag ggcggtggtt accgcctctg ctctgacggc gagcgctcgg gctttcacca
     1621 gcgaaccgtc gaacgaggcg tctgcgcctc tcccgtcgat gttttcggtg atgctgatca
     1681 gctcatcggt ctgaatctgc agggcgtcca gctctgcggc gtcgtcggcg agctggccca
     1741 gctcgttgat ttctcctgag atgcgatcca gggacgtcaa cgttgcctgg gtgcggctgg
     1801 tgaggtccgt ggttggctgg acttccgcgg gcgtcccctg ggatccggcg gcatccgggc
     1861 ttgtggtctg ctgcgctgac gcgtggccgg gccaactagc gagccagatg aagcacgacg
     1921 ccatgatcaa tctgtggcga cccagcgatc gacgcatcac catggccctt tggggaaagt
     1981 ggcgaagttt aggggcgaag cttcctggtc tcaaccggta cccaggcgcg cacgttgacg
     2041 agccgatcca cgggccaatc gcgatcgcgc gggggctcct tccagggcgg gaggatcctg
     2101 atcaggccgt gacgacttcg ctcgaatcgc cggctgccag ctgcttgagc gtgagcccgc
     2161 cgagcgcacg atctctgccg gaaccgccgg ctgcctcgat cgtcacgatg tcctcgtcag
     2221 cgcccagggc gggcgcgtag gttccgggtg gctggtatgt ctccacccgc tgaagcacct
     2281 cggcgacggt ggtggtatcc agtgggagag ccggaatgta gcaaggcttg gggtcgtccg
     2341 tctccgccag tacgtccagg gccaccagga gcgtaaggca gtgctccagc gcgttggtcg
     2401 ggaggcgata gcttgctgcc agctcgtcaa gggtataggg cttcttcttc tcgtaatgcc
     2461 ggcgaccgat cttggcgagc agcgatagca cgtagcgggt ggtcagctgc tggctgaagt
     2521 gcacctcctt cgagcccgag gcgatgacct ccggatgctg ccgatagaag gcgatgcttg
     2581 ctcctatgag aagaatcagc cagttcagat acagccagat gagaaagaat atggccgtag
     2641 caaacgtggc gtagatcagc tggtagcggg aggcgccgct ggcgtaggag gcaaatccga
     2701 agcccaggag ctcccagagg acgccggcga cgatgccgcc agttaatgcc gccgagattc
     2761 gcactctcgt attggggatg aagacgtaca ggaaggtgaa cgcgccgatg acgaagacga
     2821 acggcagaag cctgacgaca atctggatgg ccgggccgac cacggcgatc tcctggacgt
     2881 gggtcatcac ggtggtgctc atgatcgagc ccgtcagggc gatggcagta aagatgagca
     2941 gcggcccgat ggtgatggcg gagaggaagg tggtgaaacg ttccccgaac gttctgggcg
     3001 tgcgaatgcg ccaggtgtga ttgaatgaat cctcgatctt ctgcatcagc gaaaccacgg
     3061 tgtagagcag aaacagcaga ccgaccgcgc ccagcacgcc cacgttcatg ttgttgacga
     3121 aggtgatcag gttttccgtc agctcgaggg ccttttcctc gcccagaggc gccagcgctg
     3181 cacgtaaagt ctcgtcgagc tgattctgga ccccgaatcc cttgagaatc gagaagctca
     3241 gcgcgagcag cggcaccatg gagagcagcg tcgtgtacac gaggctcatg gctctcaggc
     3301 tgatgtcacc gtgaaagatg tcccggaaca ggagcatgag cgttcgggcc atgcccacca
     3361 gcacgcgctg ccatggggga gcctgccgat agtccggctc ccagagatag cgctgctcga
     3421 acgatgtgag agtgttgaga tccaagtgcg tccccctgcc cttggagtga agggaaaggg
     3481 atttcccggg tgccgtcaag gcccctcgcc caggcgggtg cgcccatacg acgcggccgc
     3541 gccgcgcgct gcttagccgt agcgaggttt gccgggctgg gctagattag ggcaaagggt
     3601 ttctggagag ggagaatgtc agacgcgcag gctcgcgggc taccgccgga cgatcccacc
     3661 acgctgatca cgccggagct gatcgagcag tatcggcgcg atggcgtcat ctgcgttcga
     3721 aacgcccttc atccggagtg gctgatgctg ctggagatgg gtctcgagcg catcatggcc
     3781 gaccccggcc agcagaagca tctgttctat cggggcgagc ctggggaatt catcgaaacc
     3841 atacgcaact tcgacgtggc gcccgagatc cggcggctca tatacgactc gcccattgcc
     3901 gacatgatcg gcaagctcat cggttccgga aacatctggc tttactcgga cgagttcttc
     3961 gtgaaggaag gcggcgggtg tgagcggacg ccgtggcatc aggatctgcc gtattggccg
     4021 ctggaaggag agcagatcgc ctcgatgtgg atctctctgg atccgctgcc ccgggaagat
     4081 tgcctggagt atgtggcggg ctcccaccag ggccccatgt tcgatggttt cgacccccag
     4141 cgggtaacgg aggacccgac ccttccgcat tacggcgagg ggctgccccc gctgccggac
     4201 atcgaggcgg atcgaggccg cttcaacatc gtctcgtggg acatcagtcc cggggacgtc
     4261 atcctcgctc atccgggcgt tctgcatggc ggtggcgcaa ccgggatcgc gggccgccgc
     4321 agagcgatca cgatccgctg ctatggggac gacgtcgtct acgccacccg gccgcccacg
     4381 cggcccacgg tgcccctcac gccgggtctc ggtctaaagc tgaaaccggg agacccgctg
     4441 cgccatcctt actatccgcg gctgcgcccg cttcccgagc accagcgggt ctgaggaggg
     4501 atcagcgggc gcatcgaccc ctgcgggtcc gctcgttgcg ctctgcccgt gtgcggcgga
     4561 tgcctatcct gcggataccg cagccagcgg ggtaggcagc tccccgctca ccagctgccc
     4621 gcctttcagc actgcctgaa cgctctcagg ccgcgtcagc acgcgaatgt cccgcgacgg
     4681 atcgtcgttc aggatcagaa ggtcggccac ctttcccggt gcgatggtcc cgatgccctc
     4741 gaagcccgtc atctcgccac cgtgcaacgt gctccatttg atcagctcca gcggcgggat
     4801 gccggcgtgc tccgcgtagc agacgagctc ttccgcgtaa gcgccgtggg gaatctgcca
     4861 gatgccgaag tcgtcgccgg ggaccagctt gacgccggca tcgaccaggc ggggcaacag
     4921 tctgcacagg ctctcaaagt ccttgaggtc ctcgctgaaa ccgaacgtgt tgccgcccag
     4981 ctcggctgcc ttgagcagca cgtagccgct gggcaacaca aaagcatcgt gttccagata
     5041 ggcctcaatg acctcttcgt ctgcctcgtc gccgtgatcg atgatggtga cgccggcacg
     5101 caggcaatcg agtatctgtt gcttgccggc gacatgagct cggacccggg cgccgtggtc
     5161 ccgggcgacc tccacgacgg ctccgatctc ctcggcgctc atgcacatgc ccgccggcgc
     5221 gccgtggccg ccgctggcga agatcttgac gatcttcaca ccgcgttcga ggtcgaacag
     5281 cacgcctttt ctgaaccctt ccgggccttg ggccatgcac gtctcctcgt tctccatggc
     5341 gctgtcagca ctcatggccg gcatgtagtt gcggctgcag ggcaccagcc gcgggccgac
     5401 cacctcgccc gcgtcgatgg
//
LOCUS       DBNHOX010000571         1734 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_110655, whole genome shotgun sequence.
ACCESSION   DBNHOX010000571 DBNHOX010000000
VERSION     DBNHOX010000571.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_110655"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1178
                     /locus_tag="AC16UZ_12560"
     CDS             102..1178
                     /locus_tag="AC16UZ_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR6247561.1"
                     /translation="MTQSSNPGGEPCVSYTDRHRYYCGIDLHARTMYLCILDHESGEK
                     LLHRNLRCDPELFLQAVEPYRDDLVVATECIFCWYWLADLCAREGITFVLGHALYMKA
                     IHGGKAKNDRVDSQKIAVLLRASALPKAYVYPPGMRATRDLLRRRLFFVRKRGELFAH
                     VRTTHHQYNLPTPSGQLLYLCNRAKVPEAFDNPIVRASVEADLALAGHYDGLIRSLER
                     TILAQAKVEDPATLDRLQTIPGVGPILALTLLYELHDIARFKRVQHFLSYARLVKSQK
                     SSGGKVLGTSGAKIGNAHLKWAFSEAAVLFLRKNPRGQKHLDRLRSKHGKGKALSILA
                     ARLGRASYFMLRRSTDFDMDRFMN"
BASE COUNT          314 a          651 c          446 g          323 t
ORIGIN      
        1 acggtaccgg cccgcgacgg tcgctgcgac agcagccact gcttgatact catgcaggcc
       61 ccctcctgga cctcccaagc atcgggagga acgatgagag cgtgacacaa tcatccaacc
      121 caggagggga accatgcgtt tcttacaccg accgtcatcg atactactgc ggcatcgacc
      181 tgcacgctcg caccatgtac ctctgcatcc tggaccacga gagcggcgag aagctcctgc
      241 accgcaacct gcgctgcgat cccgagctct tcctccaagc cgtcgagccc taccgcgacg
      301 acctggtcgt tgccaccgag tgcatcttct gctggtactg gctcgccgat ctgtgcgccc
      361 gcgaaggcat cacgttcgtg ctcggccatg ccctctacat gaaggccatc cacggcggga
      421 aggccaagaa cgatcgcgtc gattcccaga agatcgccgt cctcctgcgc gccagcgccc
      481 tgcccaaggc ctacgtctat cccccaggca tgcgcgctac ccgcgatcta ctccgccgac
      541 gcctcttctt cgtgcgcaaa cgaggcgagc tcttcgccca cgtgcgtacc acccaccacc
      601 agtacaatct gcccaccccg tccggccagc tcctctatct gtgtaaccgg gccaaagtcc
      661 ccgaggcctt cgacaacccc atcgtccgcg ccagcgtcga ggccgatctc gccctggccg
      721 gccactacga cgggctcatc cgctccctgg agcgcaccat tctggcgcag gccaaggtcg
      781 aagaccccgc aacgctcgac cgtctgcaga ccatcccggg cgttgggccc atcctcgccc
      841 tcacgctcct ctacgagctc cacgacatcg cccgcttcaa gcgtgtccag catttcctgt
      901 cctatgctcg cctggtcaag agccagaagt cctcaggcgg caaggttctc ggcacctcag
      961 gcgccaagat cggcaacgcc catctcaagt gggcattcag tgaggccgct gtcctctttc
     1021 tcaggaagaa cccccgcggc cagaagcacc tcgaccgcct ccgctccaag cacggcaaag
     1081 gcaaagctct cagcatcctc gccgctcgcc tcggccgcgc cagctacttc atgctccgcc
     1141 gcagcaccga cttcgacatg gaccgcttca tgaactgaat ccttgaggag gagaggcggt
     1201 gagcccgacg tctaactcga gccgggtacc cgatgttctc ctggtagccg ccctgtttga
     1261 ttggacatcg cctctcactc cgatccacat gcacactcgg atcctcgctt atggatgctc
     1321 tgcccctcac ccgagcccgc agataactgg ccatggcttc ggccttccag cccgtactcc
     1381 ctttgaatcg gacggcatga gggcacgcat gatcttctag agtcgcagga gcccgctcct
     1441 gctccggctc acccgccgga acgaacctca ataggtgttt ggtgcagagt cctaccggct
     1501 ccgcgcccgc tccctgaatt gaagtcagag tccccgagga cccgatgctc actcgttcgt
     1561 cgcgctgcac cagcagcgcg tcaggggata cttccgcgct cttgactccg gggggcctga
     1621 taggtgttat atgccgtcgc gattcctcgc gtttatttgt tccataccgg ccggcatcta
     1681 ccgccttcgc acgcctgagt cagagcgatc accttgccga agcgtgaccc cctc
//
LOCUS       DBNHOX010000572         7355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_946444, whole genome shotgun sequence.
ACCESSION   DBNHOX010000572 DBNHOX010000000
VERSION     DBNHOX010000572.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_946444"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="AC16UZ_12565"
     CDS             <1..387
                     /locus_tag="AC16UZ_12565"
                     /inference="COORDINATES: protein motif:HMM:NF018047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HIR6247562.1"
                     /translation="LGIATPRPVALLEARQGLATVAYLVMEDLGAARDLAQAVAEEGY
                     TAGVGRAVAGLFRGLRDAGLVHGDTKASNFLVRDASVYLIDLDAMKPGSQTRSRDVAR
                     FLANWDDAPQVCESFRGALQEAGVPL"
     gene            412..2157
                     /locus_tag="AC16UZ_12570"
     CDS             412..2157
                     /locus_tag="AC16UZ_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007939655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase family protein"
                     /protein_id="HIR6247563.1"
                     /translation="MPLIILGLSGALNHDPSAALLVDGEIVAAVEEERFLRDKHAKNR
                     MPLESARYCLRAGGISPRDVDVVAFPYAPIALSSAARWHYARRHWYAPDRALDALFNG
                     NRRYRRNRRRVLELGDQLGIDWSRTRFEPVEHHLAHASSAYHLSGFTEKTAIMGVDGK
                     GEYATTFFGYGENGRIHRIKEFYDPDSLSGVYGAMTEYLGFEMLDGEYKVMGMAPYGD
                     PDLYDLGRLIRRARDGFEVDVNYVNTVGLRRHKESGVGFYFSDKLINWLGPRRQGEVA
                     DEPYIHYAASMQKLFEEWCLELMERYLGDVLRETGRLAFAGGGALNVKLNQRIVALDH
                     VRELFVQPAAGDSGTSLGAATYVAAREGQRLSPMTHVFLGPRYDNDDCLQALRARRES
                     LHWRRLDDVPARAAELLAQGHPLAWFQGRMEFGPRALGARSILGCPSTPGIADRINEQ
                     IKFRERWRPFCPSVLDRVAAEIIQTEHPAPFMTITFDVAQEWKSRIPEVVHEDGTARV
                     HIVDPDSNPRYYALLETLETLTGNAVVLNTSLNRRGEPMVCSPEDALNMFCGSDLQFL
                     VLEDFLVSKTDEEAQ"
     gene            complement(2151..3068)
                     /gene="lpxL"
                     /locus_tag="AC16UZ_12575"
     CDS             complement(2151..3068)
                     /gene="lpxL"
                     /locus_tag="AC16UZ_12575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HIR6247564.1"
                     /translation="MSRRRKSRPRMYSPRVWGTWLIVAIGWLLARLPISAIGGLGRWL
                     GRIAYRLARSRRHIAEVNLRLCFPDLGEAARREMIRGVFQHVAVGALETMVVWLDPDR
                     DLGDRVTIEGLEHLRAAQAEGKGVLLLGGHFTSLDVMANAVSRAVDLDVIYRANKNPV
                     WEWLQVRGRSRYCDGVIERSDVRQTIARLKAGRTIWYAPDQDYGPKHSVFAPFFGAPA
                     ASITATSRLARISGSPVIFASHFRDWNTMTWHVTFSEPLDGFPTSDEVADAARVNQLI
                     ENAIRAHPDQYLWIHRRFKTRPPGEGKLY"
     gene            3132..4556
                     /gene="hldE"
                     /locus_tag="AC16UZ_12580"
     CDS             3132..4556
                     /gene="hldE"
                     /locus_tag="AC16UZ_12580"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HIR6247565.1"
                     /translation="MNLPSLQGVHVLVVGDLMLDRYWIGPAERMSQEAPVPVVRVERV
                     EEHPGGAANVALNVASLGARCTLIGYVGKDDAAEALVDRLTAAGVACDLVVVDGWSTV
                     VKLRVVSQNQQLIRTDFESALIPGEDHRALLREKVIARLLDASAMVLQDYDKGVIEDP
                     ETLIGSAIAAGVPVVVDPKRKPFARYAGAAILKPNAKELEAALGSWDDDTDLVSLSAA
                     ACRTHRLGAMVITRGAGGMTVVAADGQHRHIPALPADVYDVTGAGDTAAAALAVTTSL
                     GWDADRGAQVANVASGLVVAKAGTAAITGPELALALAGRHRVDRGALRRAQLEDAVAQ
                     ARAAGERIVFTNGCFDILHAGHVAYLEEARALGDRLVVAVNDDDSVARLKGPGRPVNS
                     LERRLRVLAGLASVDWVVGFSEDTPEALLELLKPDVLVKGGDYGPSEVVGADQVIAQG
                     GEVRVLGLVEDCSTTAIVDRIRGA"
     gene            complement(4593..5351)
                     /locus_tag="AC16UZ_12585"
     CDS             complement(4593..5351)
                     /locus_tag="AC16UZ_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6247566.1"
                     /translation="MQYRPLGNTGIEVPLIGLGTVKLGRDQGVRYPRPFTIPDARAAA
                     ALLKKARELGVNLLDTAPAYGTSEERLGALIGAERDHWIICTKVGEEFSNGVSRFDFS
                     PEHTRRSVERSLERLRTDRLDIVLIHSSGADVEILENLGTLDALRELQASGRVRAIGI
                     SHKTPDGGRLALAAGCDVIMTVLNPADREQAPIVAEAGRIGCGVLVKKALASGFAGPE
                     SLEWVAAQPGVSCIVTGTIDPEHLAANVAAVCPA"
     gene            complement(5353..6522)
                     /locus_tag="AC16UZ_12590"
     CDS             complement(5353..6522)
                     /locus_tag="AC16UZ_12590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6247567.1"
                     /translation="MSGRDWQTDAIIVGGGIAGLWLANLLVRQGYQVVVLEKDRLGAG
                     QTLASQGMIHGGLKYALTGRLTRASQAIADMPERWRSCLDGTGDLDLSALAPLAERYY
                     LFAESSSLGRLTGFFASRSLRGRIEKVEPHDYPCVFANPAFHGVVYALRDFVLDTGAL
                     VSQLNDTLRGCVFQHAVAPEDLLVEADRVVLRLPDASLTARHLIICAGAGSQPLLDGL
                     GLRRPRMQLRPLHQVVVRHEHPHPMYAHCLTGIRRAEPRLTITSHPDGDHWLWYLGGQ
                     LATDGVKLDAPALIDRARRELEACVPWLSWDDARFETLRIDRAEPEQTRGGRPDEAFA
                     AAVGSCVVCWPTKLSLVPDLGDKVLGLLDPPSAPHPGSPDLGLPAARVGRSPWET"
     gene            6608..>7355
                     /locus_tag="AC16UZ_12595"
     CDS             6608..>7355
                     /locus_tag="AC16UZ_12595"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF016311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HIR6247568.1"
                     /translation="MWRILYQVLLPLAYPLVRLRLLWRARREPEYGRRIPERLGRVPA
                     SVPRGVIWFHTVSAGETIAAAPIIDRLLSEFPESDFLVTTMTPTGSQQVIARLGGRVA
                     HCYAPYDFRGGVERFLDAVAPRLLVIFETELWPNLLEAATRRGIPVDLVNARLSERSA
                     RGYARIGALTRRMLGQLQHIACQYPAHAQRFVALGAQPGRVSVLGSVKFDLRLPDDHR
                     ARCAELARRWRLEGRPVWIAGSTHPGEDAPV"
BASE COUNT         1169 a         2406 c         2539 g         1241 t
ORIGIN      
        1 ctgggtattg ccacgccccg gccggtcgct ctgctggaag cgcgccaggg gcttgctacc
       61 gtggcctatc ttgtcatgga ggatctcggt gcggcgcggg atctggcgca ggccgtcgcc
      121 gaggaaggat acaccgcggg tgtcggacgt gcggttgccg ggttgttccg cgggctgcgc
      181 gacgcgggcc tggtgcacgg ggatacgaaa gccagcaact tcctggtgcg cgacgcaagc
      241 gtctacctca tcgacctgga tgccatgaag ccgggcagcc agacgcggtc ccgcgacgtc
      301 gcccgtttcc tggcgaactg ggacgatgcg ccacaggtgt gcgagtcttt tcgcggcgcc
      361 ttgcaggagg ccggcgtgcc gctatagtac gcgcactttc cgatcccagg tttgccgttg
      421 atcatcctcg gcctttccgg agcgctcaac cacgaccctt cggccgcgtt gcttgtcgac
      481 ggggagatcg tcgcggcggt tgaagaggaa cggtttctgc gcgacaagca cgcaaagaac
      541 cgcatgccgt tggaatcggc ccgctactgt ctccgcgccg gtgggatttc gccaagggac
      601 gtggacgtgg tcgcgtttcc ctacgcgccg atcgcgctgt ccagcgctgc gcgctggcac
      661 tacgccagac gccactggta cgcgcccgat cgggcgctcg atgcgctttt caacggcaac
      721 cgtcggtatc gccgaaaccg gcggcgcgtt ttagagctcg gcgaccagct cggcatcgac
      781 tggagccgga cccgattcga gcccgtggag catcatcttg cccacgcctc cagcgcctac
      841 cacctgagcg gctttacgga gaagacggcc atcatggggg tggatggcaa gggtgagtac
      901 gccaccacgt tcttcggcta cggcgagaac ggccgtattc accggatcaa ggaattctac
      961 gacccggact ccctgagcgg cgtctacggc gcgatgaccg aatacctcgg cttcgagatg
     1021 ctcgacggtg agtacaaggt gatgggcatg gcgccctacg gagatcccga cctatacgat
     1081 ctcgggcgcc tgatccgccg agcacgcgac ggcttcgagg tggacgtgaa ctacgtgaac
     1141 acggtgggcc tgcgacgtca caaggagtcg ggcgttggct tctacttcag tgacaagctc
     1201 atcaactggc tcggaccacg ccggcaaggg gaggtggctg acgagcccta catccactac
     1261 gccgcatcca tgcagaagct cttcgaggag tggtgcctgg aactcatgga gaggtacctc
     1321 ggggacgtgc tgcgggagac cgggcggctg gcttttgcag gcggcggagc cctcaacgtg
     1381 aagctgaacc agcgaatcgt tgccctggat cacgttcgtg agctgttcgt ccagccggcg
     1441 gccggagact cgggtacttc gctgggggcc gccacctacg tggcagcccg cgaaggtcaa
     1501 cgcctatccc ccatgaccca cgtctttctc ggtccgcgtt acgacaacga cgattgcctt
     1561 caggcccttc gagcccgtcg cgagtccctt cactggcggc gcctggacga cgtgcccgct
     1621 cgggcggcgg aacttcttgc ccaggggcat ccgctggcgt ggttccaggg ccgcatggag
     1681 ttcgggcccc gggccctcgg cgcccgcagc attctgggtt gtcccagcac gcccgggatt
     1741 gccgatcgca tcaacgagca gatcaagttc cgcgagaggt ggcggccttt ctgccccagc
     1801 gtgctcgatc gggtcgcagc ggaaatcatc cagaccgagc atcccgcgcc tttcatgacc
     1861 attaccttcg atgtggcgca ggaatggaaa tcgcggatcc cggaagtcgt tcacgaggac
     1921 gggaccgccc gtgtccatat cgtggatccg gacagcaatc cccggtacta cgcgttgctc
     1981 gagaccctgg aaacgctcac gggcaacgcg gtggtgctca acacgtctct caaccggcgg
     2041 ggagaaccca tggtctgctc gccggaagac gcgctgaaca tgttctgcgg gtccgatctt
     2101 cagtttctcg tgctcgagga ttttctcgtc agcaagacgg acgaggaagc tcagtagagc
     2161 tttccctcgc ccgggggacg cgtcttgaac cggcgatgaa tccagaggta ctgatccggg
     2221 tgcgcgcgga tcgcgttctc gatcaattgg ttgacccgtg ccgcgtcggc cacctcgtcg
     2281 ctggtcggga acccatcgag cggctcgctg aacgtgacgt gccaggtcat cgtgttccag
     2341 tcgcgaaaat ggctggcaaa gatcacgggc gagccggaga tccgcgcgag gcgagacgtg
     2401 gcggtgatgg aggcggcggg cgcgccgaag aaaggcgcaa agacggagtg cttgggcccg
     2461 tagtcctggt ccggcgcgta ccagatcgtg cgccccgcct tgagacgcgc gatcgtctgg
     2521 cggacgtcgg agcgctcgat gacgccgtcg cagtagcggc tccgccctcg aacctgcagc
     2581 cactcccaga cgggattctt gttggcgcgg tatatgacat cgaggtctac ggcgcgggaa
     2641 accgcattgg ccatcacgtc cagggacgtg aagtggccgc ccagaagcag cacgcccttc
     2701 ccttcggcct gcgcggcccg cagatgctcg aggccttcga tggtcactcg gtcgccgagg
     2761 tcgcggtcag ggtccagcca gaccaccatg gtctccaggg cgcccaccgc aacgtgctga
     2821 aacacgcccc ggatcatctc ccgtctcgcg gcttctccca gatcgggaaa acacagcctg
     2881 aggttgacct cggcgatgtg tcgtcgtgac cgggcgaggc ggtaggcgat acggcccagc
     2941 catcgcccga gccccccgat cgcagatatg ggcagacgcg cgagcagcca gccgatggca
     3001 acgatgagcc aggtccccca gacgcgagga gagtacattc ggggtcggct ctttctgcgc
     3061 ctgctcatga ggttccaccg gcggggtagg cgaattgtaa cggggaggcc ctgtgatacc
     3121 atccacgccc catgaatctg ccgtcgctgc aaggggttca cgtcttggtg gtcggcgatc
     3181 tcatgctgga ccgctactgg attggtcccg ccgagcggat gtctcaggaa gccccggtcc
     3241 cggtggtcag ggtggagcgg gtagaggagc atcccggagg cgcggccaac gtggccctca
     3301 acgtcgccag tctcggcgcg cggtgcaccc tcattggtta cgtaggtaaa gacgacgcgg
     3361 ccgaagccct cgtcgacagg ctcacggcag ccggggtcgc ttgcgacctg gtcgtggtag
     3421 acggttggtc caccgtggtc aagctgcggg tggtcagcca gaaccagcag ctcatccgca
     3481 ccgatttcga aagcgctctg atcccgggcg aggaccatcg cgcgctgctg cgtgaaaagg
     3541 tcattgcccg gctgttggat gcttcggcca tggtgcttca ggattacgac aagggcgtga
     3601 tcgaggaccc cgaaacgctg atcggctccg ccatcgcggc gggcgtgccg gtggtggtgg
     3661 accctaaacg caaacccttt gcccgctatg cgggagccgc catcctcaag cccaatgcaa
     3721 aggagctcga ggcggcgctc ggctcgtggg acgacgacac ggatctcgtc tcattgtcgg
     3781 cggcggcctg cagaacccat cgcctgggtg ccatggtcat cacccggggt gccggcggga
     3841 tgacggtcgt tgcggccgac gggcagcacc ggcacatccc ggccttgccg gcggacgttt
     3901 acgacgtgac gggcgcgggg gataccgctg ctgccgcgct cgccgtgacc accagcctgg
     3961 gctgggatgc ggaccgcggt gcccaggtgg cgaacgtggc gagcgggctg gtggtggcca
     4021 aggcgggaac ggcggcgatc acggggcccg agcttgcgct tgcgctggcc gggcgtcatc
     4081 gggtggaccg cggtgctctg cgcagggcgc agctggagga cgcggtcgct caggcgcgag
     4141 cggccggcga gcggatcgtc ttcaccaacg gctgcttcga catccttcac gcgggccacg
     4201 tcgcgtatct ggaagaagcc cgggcgctgg gcgatcgcct ggtggtggcc gtcaacgacg
     4261 atgactcggt ggcgcgtctg aaaggcccag gtcggccggt gaattctctc gaacgacgct
     4321 tgcgtgtgct ggccgggctc gcgtcggtcg actgggtcgt cgggttctcg gaggatacgc
     4381 ccgaagccct gctggagctg ttgaaaccgg acgtcctggt caaggggggc gactacgggc
     4441 ccagcgaggt ggttggcgcg gatcaggtca tcgcccaggg cggcgaggtg cgcgtgctcg
     4501 gtctggtgga ggattgctca accactgcca tcgtcgatcg gatccggggc gcttaggcgc
     4561 cgagcccggg atgtgccggc gcggggccgg cttcaagccg ggcagacggc ggccacgttg
     4621 gcggccaggt gctccggatc tatggttccg gtgacgatgc aggacacgcc gggctgtgcc
     4681 gccacccact ccagtgattc gggccccgcg aagccgcttg ccagggcttt ctttacgagc
     4741 acgccgcagc cgatgcgtcc ggcctccgcc acgatggggg cctgctcgcg gtcggccggg
     4801 ttcagcaccg tcatgatcac gtcgcagccg gcggccaggg caagccggcc gccgtcgggg
     4861 gttttgtggg agatcccgat ggctcgcacg cggcccgaag cctgcagctc ccggagcgca
     4921 tccagcgtgc ccaggttttc caggatctcc acatccgctc cgctggagtg gatgaggacg
     4981 atgtccaggc ggtccgtgcg taggcgctcg agagacctct cgacgctgcg ccgtgtgtgc
     5041 tcgggcgaga agtcgaaacg ggacacgccg ttcgagaact cctcgccgac cttcgtgcag
     5101 atgatccagt ggtcccgctc ggcaccgatc agcgcgccaa gccgttcctc gctcgtgccg
     5161 taggcggggg cggtgtcgag gaggttaacg cccagttccc tggctttctt gagcagggcg
     5221 gcggcggccc gggcgtcggg aatggtgaac gggcgcggat acctgacgcc ctggtcgcgg
     5281 cccagcttga ccgtgccgag accgatgagc ggcacctcga tgcccgtgtt gccgagggga
     5341 cggtactgca tgctaggttt cccagggtga gcggccgacc cgggccgcgg gcaggcccag
     5401 gtccggcgag ccggggtggg gagccgatgg cggatccagc agcccgagca ccttgtcgcc
     5461 gagatcgggt accaggctca gcttggtggg ccagcacacc acgcagcttc cgacggccgc
     5521 tgcgaacgcc tcgtcgggcc taccccctcg ggtctgctcg ggctctgccc ggtcgatgcg
     5581 cagggtctcg aatcgcgcat cgtcccacga aagccagggg acacaggctt cgagctcgcg
     5641 ccgggcccgg tcgatcaggg cgggggcatc tagcttcacg ccgtccgttg ccagctgccc
     5701 gccgagatac cacagccagt gatcgccgtc ggggtggctg gtgatggtca ggcgcggctc
     5761 cgcgcggcgg atcccggtca ggcagtgagc gtacatggga tgcggatgct catgtcggac
     5821 caccacctga tgcagcgggc gcagctgcat gcgcggtctc cgcagcccca ggccgtcgag
     5881 cagcggctgg gagccggcgc cagcgcagat gatgaggtgt ctcgccgtca acgacgcgtc
     5941 cgggagccgg agcaccaccc gatccgcctc gacgagcaga tcctccgggg cgacggcgtg
     6001 ctgaaagacg catccccgca gcgtgtcgtt cagctgcgag acgagggcgc cggtgtcgag
     6061 cacgaagtcg cgcagcgcgt agacgacgcc atggaaggcg gggtttgcga aaacgcaggg
     6121 gtaatcgtgc ggctcgacct tctcgatccg tccgcgcagg gagcggctgg caaagaaccc
     6181 cgtcaacctg cccaggctcg acgactcggc aaacagataa taccgctcgg ccaagggagc
     6241 aagggcggaa agatccaggt cgcccgtccc gtccaggcag cttcgccagc gctcgggcat
     6301 atcggcaatg gcctgcgagg cccgggtgag ccggccggtg agcgcatact tcagcccgcc
     6361 gtgaatcatg ccctggctcg ccagcgtctg gccggcgccc agccggtcct tttcgagtac
     6421 taccacctga tatccctgcc gcaccaggag gttggccagc cacaggccgg cgatgccgcc
     6481 cccgacgatg atcgcgtccg tctgccagtc tcgcccgctc atgggagcgc gagcctacgg
     6541 tttcgtccgc agggccgcaa tcccgggcat tcttgacggg gaccgcgcgc ctgcgcaggc
     6601 ttgcgccatg tggcggatcc tttatcaggt gctcctcccc ctggcctatc cgctggtccg
     6661 gttgcggctg ctgtggcgcg cgcgccgtga gcccgagtac gggcgacgta tccccgagcg
     6721 gttggggcgg gtgccggcat cagtgcccag gggcgtcatc tggttccaca ccgtctcggc
     6781 cggcgagacc atcgctgctg cgcccatcat cgatcgtctg ctaagcgagt ttcccgagag
     6841 cgactttctg gtcaccacca tgacgccaac cgggtcccag caggtcatcg cccgcctagg
     6901 cggacgggtc gctcactgct acgcgcccta tgacttccgc ggcggcgtgg agcgcttcct
     6961 cgacgccgtc gccccccggc tgctggtgat cttcgaaacc gagctgtggc ccaacctcct
     7021 ggaggcagct acccggcggg gtattcccgt tgatctggtc aacgccaggc tgtctgaacg
     7081 atccgcgcgc ggctatgccc gaatcggggc cctgacgcgc agaatgctcg ggcagctgca
     7141 gcacatcgcc tgccagtacc cggcccacgc ccagcgattc gtcgcccttg gcgcccagcc
     7201 cgggcgggtg tccgtgctcg gcagcgtcaa gtttgacctg cgcctccccg acgaccaccg
     7261 cgcccgatgc gccgagctcg cccgtcgatg gcggctggaa ggccgtccgg tctggatcgc
     7321 ggggagcact catcccggtg aggatgcgcc ggtcc
//
LOCUS       DBNHOX010000573         1552 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1588523, whole genome shotgun sequence.
ACCESSION   DBNHOX010000573 DBNHOX010000000
VERSION     DBNHOX010000573.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1588523"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1333)
                     /locus_tag="AC16UZ_12600"
     CDS             complement(62..1333)
                     /locus_tag="AC16UZ_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HIR6247569.1"
                     /translation="MKEDTTVVPFRHGDSIDDPLTEIAREGARRLLAEALKAEADAFV
                     ARFTDDLLHDGRQRVVRHGYGPERSIQTGIGAIDVRRPKVRDRALDQPEGGKVRFTSN
                     ILPRWARRSKSLDALLPVLYLRGISTGDFQEALGALLGTDAPNLSPGVISRLTAGWQE
                     GYDAWQRRDLSARHYVYIWADGVYLQARMEPQAECILVIIGATPEGKKELVGFQVGIR
                     ESTQSWRELLVDIKARGLSTAPQIAVGDGAMGFWKALDEVFPGTRHQRCWFHKISNVL
                     NKFPKSMAPAVKSDLQNIHHAPTRADAVAAIETFTEKYGVKYAPAVTCLTKDIDALLA
                     FYDFPAEHWDHLRTANPVESVFATVRHRTVRTKGALSQKTAKLMVFKLVQAASKTWRR
                     LNGRNQLPKLIEGIKFTDGVAVNDVAETSAA"
     gene            complement(1407..>1552)
                     /locus_tag="AC16UZ_12605"
     CDS             complement(1407..>1552)
                     /locus_tag="AC16UZ_12605"
                     /inference="COORDINATES: protein motif:HMM:NF014522.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HIR6247570.1"
                     /translation="PKGTVEMLLKPENKDKLIAVLTYHVVAGKVMSGDIAGKRVGVMA
                     EFG"
BASE COUNT          267 a          503 c          470 g          312 t
ORIGIN      
        1 ccgtgaaggg tctcaggcgc tacgcgctga gctatgctgg aaattgggtg acgcggccaa
       61 atcaggcggc gctggtttcg gctacatcgt tcacggcgac gccgtcggtg aacttgatcc
      121 cttcgatgag cttcggcaac tgattcctcc cgttcagcct ccgccaggtc tttgaagctg
      181 cctgcacgag cttgaacacc atcagcttgg ccgttttctg tgacagcgca cccttggtcc
      241 tgaccgttcg gtgccggact gtggcgaaga cgctctcgac agggtttgcg gttcgcagat
      301 gatcccagtg ctcggcaggg aaatcgtaga aggcgagcag cgcgtcaata tccttggtca
      361 gacatgtgac cgccggggcg tatttgactc cgtatttctc ggtgaacgtc tcgatcgcgg
      421 ccacggcgtc agcccgggtc ggggcatggt ggatgttctg gagatccgat ttcaccgccg
      481 gcgccatcga tttgggaaac ttgttcagca cgttcgatat tttgtggaac cagcagcgct
      541 gatgacgggt gccggggaag acctcgtcga gcgccttcca gaaccccatg gcgccgtcac
      601 cgacggcgat ctgtggagcc gtgctcagcc cgcgcgcctt gatgtcgacc agaagctcgc
      661 gccagctctg ggtgctctcg cggatgccaa cctggaagcc gacaagctcc ttcttcccct
      721 caggtgtcgc gccgatgatg accaggatgc actcggcctg gggctccatg cgtgcctgga
      781 ggtagacgcc gtcagcccag atgtagacgt aatggcgagc cgagaggtcg cggcgctgcc
      841 aggcgtcgta gccctcctgc caaccggctg tcagccgcga gatcacgccc ggcgacaagt
      901 tcggcgcgtc cgtgccgagc agcgcgccca gcgcttcctg gaagtcgccg gtggagatac
      961 cgcgcaggta gagcaccggc agcagcgcgt ccaagctctt cgagcgtcgc gcccatctcg
     1021 gcagaatgtt cgaggtgaag cgcaccttcc cgccctcggg ctggtccagg gcccggtccc
     1081 ggaccttggg gcggcggaca tcgatcgcgc cgatcccagt ctgaatggac cgctccgggc
     1141 cgtaaccgtg cctgaccacc ctctggcgcc catcgtgcag caggtcgtcg gtaaatctgg
     1201 cgacgaaggc gtccgcctct gccttcagcg cttcggccag caaccgccgc gctccttcac
     1261 gggcaatctc tgtcagcggg tcatcgatgg aatccccatg acgaaacggg acaacggtcg
     1321 tatcttcctt catggcgtat cgctcccttc gtggaggttt tggcaggctt gtcacccgcc
     1381 acgatacgcc gccttctcag gcgccatcac ccaaattcag ccataactcc tacgcgcttg
     1441 ccagcaatgt cacctgacat caccttgcca gcgaccacat ggtacgtcag aaccgcaatg
     1501 agcttgtctt tgttctcggg cttcagcagc atctcgacgg tgccctttgg aa
//
LOCUS       DBNHOX010000574         2137 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1781053, whole genome shotgun sequence.
ACCESSION   DBNHOX010000574 DBNHOX010000000
VERSION     DBNHOX010000574.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1781053"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          345 a          793 c          643 g          356 t
ORIGIN      
        1 gagctgtcct ttgaccactt ccgtcccttc ggctacctgg gtctccagca cgcgcccggg
       61 catcggcgcg agcatgcccg agcccagatc accgccgccg gctagcggat agcgcgggag
      121 ctccaccagc tccacgtttc ccgagctgcc gtgaaccatc caggtatctc cgagccgccc
      181 aaccaggaat ctcaggcgct ggccgtttat ctccagatcc gtttctgcgc cgccggctcc
      241 gtgaacgcga accagcttct ccgattcgtc gcccacgaac cggaacatcc cgtcacgacg
      301 cgcccggtag cgtagctgca cttccccggc tcctgccgag tagcccaccg tctccatggg
      361 catggtggag ttccgccagc cgccgggtat ggttcccatg acccgcgcgc tcttccggcg
      421 ggcgtctcgc ccttcgatgg cggcggcaat ggccgcgttc accagctcat cccggccgat
      481 gcatctgacc actgccggcc gaacgcgctc gatgaaatcg gtggtggtgt cgccggccag
      541 aaactccggc gtccgcagcg tcgccaccag gaaatcccgg ttggtggtga gcccctggat
      601 gcgcgtcgtc tccagcactc gcgccagccg ggtcgccgct tcccggcgtg tcggcgcgtg
      661 cacgatgact tttgcgatca tgggatcgaa ctcggtgccg atgaccgagc cggactccac
      721 cccggaatcg aaacgggcgc tgcccgcccg tgacggcgcc caggcgacga cggtgcccgg
      781 tgacggcaga aagtctcggc cggggtcttc cgcgtaaagc cgcgcctgga tggcatggcc
      841 ggagatggcg agctcttccg gggtgaagga aagccgctcc ccctcggcaa cacgaatctg
      901 ctcccgcacc agatcccaac cgatcacggc ctccgtgacc gggtgctcca cctgaagccg
      961 cgcgttcacc tccagaaagt agaactcgtc gccgctcagc agaaactcca ccgttccggc
     1021 agacgtgtag ccgagcttct ttgctgccgc caccgcggcc gcacccatgc gggctcgaag
     1081 atccggcgtg accgccggcg agggcgcttc ctcgatgatc ttctggtggc gccgctggat
     1141 ggagcactcg cgctcgaagc agtgcagcac gtttccgtga ccatcaccga gaatctgcac
     1201 ctctacgtgt cgcgagctct ccagccactt ctcgaaaaac accgtatcgt caccgaagga
     1261 ggacgcggct tcgcgcttcg cgcccagcac cgcgtcggcc agcccgccct cgtcctcgac
     1321 gaccctcatg ccccgcccgc cgccgcccgc ggaagccttc accagcaccg ggtagccaat
     1381 ctgcgatgcg gcttccgcaa gatccgtcgc ctcggtgatc tccaccgcag gcagggtagg
     1441 cacgccggct tctaccatga gccgctttgc cgacagcttg tcccccatga gcccgatcac
     1501 gcgaggggcc gacccgatcc accgcacccc ctcgtccacc acggcctggg cgaaacgctc
     1561 gttctccgag agaaatccat acccggggtg cacggcgtca gcgccgctct ggcgacaggc
     1621 ttcgatgacc tttgggatgt cgagataggt ctcggtactg gaacggcccc cgagcgcaat
     1681 ggccgtatcc gcctcgcgca cgtatggcgc atccgcgtcc gcatccgcgt acacggcaac
     1741 ggtgccgatt ccgagctcgt gggccgtgcg gatgatgcga cgggcgatct cgccgcgatt
     1801 agcgacgagg atccgcgaga tcggcctgct cacatccgcc acgtgccgaa ctccttggtc
     1861 ccttccaccc gattgttgaa gcaggcagac agggcaatcg cgaccacggt gcgagtgtcc
     1921 cgggggtcga tcatgccgtc gtccgagacc cgggaggttg cgtacagccc cttggagcgc
     1981 tcttcggccc aatgctcggc gacggccgcc cggctcgcat cggcctcctc gtcgaattcg
     2041 atgccacgcc gggccgccgc cgcgcggcca acgatgctca tcacgcccgc catctgcttc
     2101 gggcccatga cggcgatctt ggcgaacggc cagatga
//
LOCUS       DBNHOX010000575         5241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_431673, whole genome shotgun sequence.
ACCESSION   DBNHOX010000575 DBNHOX010000000
VERSION     DBNHOX010000575.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_431673"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..2616
                     /locus_tag="AC16UZ_12620"
     CDS             154..2616
                     /locus_tag="AC16UZ_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6247571.1"
                     /translation="MQSRAPMGSLLRAFVGLLAITGCGIVSTVAAEERDASGNIEEIV
                     VTTKRGTVENVQEMSEAVTAFAGPSLEREFAVTLEDFNHAVPNVQLEHVGLFQAASSF
                     SMRGIGTAGIESFADPVVAVFVDDVYYSRNAVSLLDLFDIESVTALRGPQGTLYGRNA
                     FAGAIVVRTKRPDLERRELELHVDVGNHGRRNTGIVGNLPLGDKAAFRLAANYHELDG
                     FYKNDGVVLESYDGVNLVTRVDEDKKGDGENGERSILIRPSLRFEPNDAWRIDIIGEY
                     WEDKGDGTGNWSQCYEPGSLPPPLGNGPSGNPDVHTGLFGFPCKDPFGDQRFGIEGDG
                     SDPYEVGFNMSPNQTKQDIWGITLDTSYETDAGTFTLVVNHRDVEEDIVSDTDGFNWN
                     LFSSSRVQDFKSTQVEARYANTFNDNIDFLGGLFYLSDEYAVQQFLWIFLDSELFGGG
                     GFGRENPAVSWGSNEQERYSIAGYAQIDWHLTDQWTLNLGGRYSFEKKKDVLGMAIND
                     SVCPEGETPATALCNGVPFSGNDPTDVTDIDPSVRFGPVSEDWNSFSPRVGLEYRVTD
                     DVMLFGFWQRAFKSGGFVNNAGTLDVFQSPYDDEQVDNYEFGIRSELFDNRLRLNANI
                     FFAEYKDLQRGVIREANTSTGQETFVDNAAGAESFGAELSFSFVPVAGLNINGNIGYL
                     DIEYKGFVADLTGDGVETDNSDLDLVRAPKWDMSIGASYEFDLADMGNLTIGARFSYT
                     DDMVLTTPNDVGFYRDELTTIDAQAVWESANRRYRFSVWGKNLSDNIERLGGTPVATL
                     FAFAAPTQPRQYGATLAVMLGD"
     gene            complement(2670..3053)
                     /locus_tag="AC16UZ_12625"
     CDS             complement(2670..3053)
                     /locus_tag="AC16UZ_12625"
                     /inference="COORDINATES: protein motif:HMM:NF016211.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HIR6247572.1"
                     /translation="MRRKTTKLLFITGGWLSLALGIAGIPLPLLPTTPFLLLAAFCFA
                     EGSDRLHDWLVNHVHFGPPIRRWREHRAVSRRTKWMGSLSLLAVLAVSLGFGAPLWLL
                     AVQGCVVVGGGAFLWTLPEPPAEEQ"
     gene            3137..4402
                     /locus_tag="AC16UZ_12630"
     CDS             3137..4402
                     /locus_tag="AC16UZ_12630"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6247573.1"
                     /translation="MSGNRPTIDPAVFASAEYAADPYPTLKLLRDHYPVYNVPGTDNW
                     IITRYEDVVNGFRDTENFSASPNGESIGAVFGPTLMEYDGDDHTKLRNIVAPQFVGKK
                     LKALLPTVGRNAMALINAFTELHARRIAAEAAAVGEIDIVDDFATRLPLNVILDVLDL
                     PQEAHEMFHQWYPAMMNGINGGPELRALGVAANAEYHAYLDPLIDARSRNPADDLLSR
                     LAVAVVDGQRMTKEEIKSFASLILVAGAETTDKAIANLWYQLLANPEQWKAVQADPDL
                     LDQAFSEMMRIHPPASAQARRAKRDVELHGKVIPAGAYVQLSIYGANRDERVFRDPDR
                     FDLFREDLYFGKELRSGYHQQGMAGHLGFGLGKHFCVGYQLARAETVIGTRMLMEVIR
                     NPRFKTGSKPTPIAIRIEPWSLPLEFDVA"
     gene            4426..5196
                     /locus_tag="AC16UZ_12635"
     CDS             4426..5196
                     /locus_tag="AC16UZ_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR6247574.1"
                     /translation="MTVDVRQVGRVTVVTIDRPEVRNAVDGPTADALAEAFSAFDAND
                     ASDVAVLTGGDGVFCAGADLKAVAAGAGPRLRETGNGPLGCTRMLLSKPVIAAVEGFA
                     VAGGLELALWCDMRVAASDAVFGVYCRRWGVPLVDGGTIRLSRLIGHSHALDLILTGR
                     GVSGDEAKAMGLANRIAPPGEALEAAMKLARSLTEFPQLCLRNDRLSSYRQWSMPLED
                     ALREETRLGLEVIRSGETREGAARFAGGKGRHGDFGDI"
BASE COUNT          968 a         1749 c         1698 g          826 t
ORIGIN      
        1 acttgtcgac ggccgtcgtt cgcccagcgg ccgtgtgggc aacgcggccg ttgacaaccc
       61 ccgcggtcgg agggcacagt ctgccgaggg cggtggaagg ctcaagggaa gagcccgtct
      121 accggggtca gagagaaccg aggggagatc accatgcaat cacgagcacc catggggtcg
      181 ctgttgcggg catttgtcgg cctgctggcg attaccggct gcggcatcgt cagcaccgtc
      241 gctgccgagg agcgggacgc gtcaggcaac atcgaagaga tcgtcgtcac gacgaagcgc
      301 ggcacggtgg agaacgtgca ggaaatgtcc gaagccgtca cggccttcgc gggaccgagc
      361 ctggagaggg agttcgccgt gacgctggag gacttcaacc acgccgtgcc caacgtgcag
      421 ctcgagcacg tcggcctgtt ccaggccgcg tcgtcgttca gcatgcgtgg tatcggcacc
      481 gccgggatcg agagctttgc cgacccggtg gtcgccgtct tcgtcgacga cgtgtactac
      541 tccagaaacg ccgtctccct gctcgacctg ttcgacatcg agtcggtcac cgccctgcgc
      601 ggccctcagg gaaccctgta cggcaggaac gccttcgcgg gcgccatcgt ggtgcgcacc
      661 aagcggcccg acctggaacg ccgcgagctc gagcttcacg tcgacgtggg caaccacggg
      721 cggcgcaaca cgggcatcgt cggcaacctg ccgctcggcg acaaagccgc gttccggctt
      781 gccgccaact accatgagct ggacggtttc tacaagaacg acggcgtggt tctcgaaagc
      841 tacgacggcg tgaatctggt gacccgggtc gacgaggaca agaagggcga cggcgaaaac
      901 ggcgagcgct cgatcctgat ccggcccagc ctgcgcttcg agcccaacga cgcctggcgc
      961 atcgacatca tcggcgagta ctgggaagac aagggcgacg gtaccggcaa ctggtctcag
     1021 tgctacgagc cgggcagcct gccgccgccc ctggggaacg ggccgtcagg aaatcccgac
     1081 gtgcacacgg ggctctttgg gttcccctgc aaggacccct tcggcgatca gcgtttcggc
     1141 atcgagggcg acggcagcga cccctacgag gtgggcttca acatgtcgcc gaaccagacc
     1201 aagcaggaca tctggggcat cactctggac accagctacg agaccgatgc cggcacgttc
     1261 accctggtgg tgaaccatcg ggacgtggag gaagacatcg tcagcgacac cgacggcttc
     1321 aactggaacc tcttctccag cagccgggtc caggacttca agtccaccca ggtggaggcg
     1381 cgctacgcca acaccttcaa tgacaacatc gacttcctgg gcggcctttt ctacctctcg
     1441 gacgagtacg ccgtgcagca gttcctgtgg atcttcctgg acagcgaact cttcggtggc
     1501 ggcggcttcg gccgcgagaa cccggcggtc agctggggca gcaacgagca ggagcgctac
     1561 tccatcgccg gctacgccca gatcgactgg cacctgaccg atcagtggac gctgaacctg
     1621 ggcggccgct acagcttcga gaagaagaag gacgtgctgg gcatggccat caacgacagc
     1681 gtctgcccgg agggtgagac gccggcaacg gccctgtgta acggcgtacc cttctcgggc
     1741 aacgacccca ccgacgtcac cgacatcgac ccgtccgtgc gcttcggacc cgtcagcgag
     1801 gactggaact cgttctcgcc gcgggtgggc ctggagtacc gggtcaccga cgacgtgatg
     1861 ctgttcggct tctggcagcg cgccttcaag tccggcggct tcgtcaacaa cgcgggcacg
     1921 ctggacgtgt tccagagccc ctatgacgac gagcaggtgg acaactacga gttcggcatc
     1981 cgcagcgagc tcttcgacaa ccggctgcgc ctgaatgcca acatcttctt cgccgagtac
     2041 aaggacctgc agcggggcgt gatccgggag gcgaacacct ccaccggcca ggagaccttc
     2101 gtggacaacg ccgcgggggc ggagtctttc ggggccgaac tcagcttcag cttcgtcccc
     2161 gtggccgggc tgaacatcaa tggcaacatc ggctacctgg acatcgagta caagggcttc
     2221 gtcgctgacc ttacgggcga cggcgtggag accgacaaca gcgatctcga tctggtgcgg
     2281 gcgcccaagt gggatatgag catcggtgcg agctacgagt tcgacctggc cgacatgggc
     2341 aacctgacca tcggcgcccg gttctcctac acggacgaca tggtgctcac cacgcccaac
     2401 gacgtgggct tctaccggga cgagctgacc accatcgacg cccaggccgt ttgggagtcg
     2461 gccaaccgcc gctaccggtt ctcggtgtgg ggcaagaacc tgtcggacaa catcgagcgc
     2521 ctgggcggta cgcccgtggc cacgctgttc gcattcgccg cccccaccca gccgcgccag
     2581 tacggcgcga ccctggcggt gatgctcggc gattgagcca accgggctcg gagcgggcag
     2641 aacgcccgct ccgtcccccg gcccctgctc tactgctcct ctgccggcgg ctccggaagg
     2701 gtccacagga acgccccgcc gccaaccacg acgcatccct gcaccgccag cagccacagc
     2761 ggcgcaccga acccgaggct cacggccaga acggccagca gactcaagct gcccatccat
     2821 ttggttcggc gggaaaccgc gcggtgttcg cgccagcgcc ggatgggcgg tccgaagtga
     2881 acgtgattga cgagccagtc gtgcagacga tcggatcctt cggcgaagca gaaggcggcc
     2941 agcagcagga aaggcgtggt gggcagcaac ggcagcggga tcccggcgat gccgagagcg
     3001 aggctcaacc aacctccagt gatgaacagc agcttcgtcg tttttctgcg catgctcgaa
     3061 caggtcctgg ggcagcctcc cgctgtttgt gcaatcatca atcctaatcg aatcttgcga
     3121 tgaacaggac gccaccatga gcggaaaccg gccaaccatc gatccagccg tcttcgccag
     3181 cgcggagtac gcggcggatc cctacccgac gctgaagctg ctgcgcgatc actacccggt
     3241 ctataacgtt ccaggcaccg ataactggat catcacccgc tacgaggacg tggtgaacgg
     3301 cttccgggat accgagaact tttccgcatc tcccaacggg gaatcaatcg gcgcggtgtt
     3361 tggcccaacg ctcatggagt acgacggcga cgaccacacc aagctgcgca acatcgtcgc
     3421 gccgcagttc gtcggcaaga agctcaaggc cctgctgccc accgtcgggc gcaacgccat
     3481 ggctctcatc aatgccttca ccgagttgca cgcccgccgg atcgcggccg aggcggcggc
     3541 ggtgggcgag atcgacatcg tcgacgactt tgccacccgg ctgccgctga acgtcatcct
     3601 cgacgtcctc gacctccccc aggaagccca cgagatgttt caccagtggt atccggccat
     3661 gatgaacggc atcaacgggg ggccggagct gcgggctctc ggcgtggcgg ccaacgccga
     3721 gtatcacgcc tacttagatc cgctcatcga tgcccgtagt cgcaaccccg ccgacgacct
     3781 gctctcgcgc ctagccgttg cggtggtgga cggccagagg atgaccaagg aggagatcaa
     3841 gtcctttgcc agcctgatcc tggtggccgg cgcggagacc acggacaagg ccatcgccaa
     3901 tctctggtac cagctgctgg ccaacccgga gcagtggaag gccgtgcagg cagaccccga
     3961 tctgctggac caggccttct cggagatgat gcgcattcat cccccggcaa gcgcccaggc
     4021 gcgacgggcg aaacgggacg tggagctcca cggcaaggtg atccccgccg gcgcgtacgt
     4081 gcagctgtcc atctacgggg ccaaccggga cgagcgagtg ttcagggatc cggatcgttt
     4141 cgatctgttt cgtgaggatc tctacttcgg caaggagctg cgctccggct accaccagca
     4201 gggcatggcc gggcatctcg gctttggcct cggcaagcat ttctgcgtgg gctatcagct
     4261 ggcgcgggcg gagaccgtca tcggcacccg catgctcatg gaggtcatcc gcaacccccg
     4321 tttcaagacc ggctcgaaac ccacgcccat cgccatccgc atcgagccct ggagcctgcc
     4381 cctggaattc gacgtggcct gaagccgccg gaaaggggga gagtgatgac cgtagacgtt
     4441 agacaggtcg gccgcgtcac cgtggtcacc atcgaccgcc ccgaagttcg caacgccgtg
     4501 gacggtccca ccgccgacgc cctggccgag gcgttttcgg cattcgacgc gaacgacgcg
     4561 tcagacgtcg ccgtgctcac cggaggcgat ggcgttttct gcgcgggcgc agatctgaaa
     4621 gccgtcgccg ccggcgccgg gccccgattg cgcgagaccg gcaacgggcc cctcggatgc
     4681 acgcgcatgc tgctctccaa gcccgtcatc gcggcggtcg aggggttcgc cgtggccggc
     4741 ggcctggagc tggcactgtg gtgcgacatg cgggtggcag cctccgatgc cgtcttcggc
     4801 gtctactgcc ggcgctgggg cgtaccgctg gtggacggcg gcaccatccg gctgtcccgg
     4861 ctcatcggcc acagccacgc gctggatctg atcctcacgg gccggggcgt ctcgggggac
     4921 gaggcgaaag ccatgggtct ggccaaccgc atcgcgccgc cgggtgaagc cctcgaagcg
     4981 gccatgaagc tggcccggag cctcacggaa tttccccagc tgtgcctgcg caacgaccgg
     5041 ctttcttcct atcgccagtg gtcgatgcca ctggaagatg cgctcaggga ggagacgcgt
     5101 ctgggactgg aggtcatccg atccggcgag acccgcgagg gcgctgcccg gttcgccggc
     5161 ggcaagggcc gtcacggcga ctttggcgac atatagcgcc cattggtcat gcgcaagggg
     5221 agcagaaccc ggctgacgct c
//
LOCUS       DBNHOX010000576         3039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_175864, whole genome shotgun sequence.
ACCESSION   DBNHOX010000576 DBNHOX010000000
VERSION     DBNHOX010000576.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_175864"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="AC16UZ_12640"
     CDS             complement(<1..803)
                     /locus_tag="AC16UZ_12640"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:0140657 - ATP-dependent activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6247575.1"
                     /translation="MLKINALSKTYPNGVRALDGVDLEIAKGMFGLLGPNGAGKSTLM
                     RTIATLQSPDSGSIHFNDVDVLADPNALRRVLGYLPQEFGAYPRTSPEVMLDHIAALK
                     GIGGRGARKALVEQLLVQTNLWDVRKKSIDSFSGGMRQRFGIAQALIGDPQLVIVDEP
                     TAGLDPAERRRFHNLLARIGEEVVVILSTHIVEDVAELCPNMAVMGGGRILVQGEPKE
                     LTGRLAGRLWSKVVPRDEAEGLRRSLPVVSTRLVAGQVELRVLSEQDPEG"
     gene            964..2397
                     /locus_tag="AC16UZ_12645"
     CDS             964..2397
                     /locus_tag="AC16UZ_12645"
                     /inference="COORDINATES: protein motif:HMM:NF005686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6247576.1"
                     /translation="MSELARQAALDTAGMIRRGDASAVEVMDAHYDRIEILNPQINAL
                     VNVLPREAALELARQADRARSRGEPCGPLHGLPMAVKDTHDAAGFPTTWGFVPWAERV
                     ARHDGAVAARQRAAGALFIAKSNVPEFALGSHTFNNLFGTTSNPYDLSRTAGGSSGGA
                     AAALATGMLPLADGSDMGGSLRNPAGYCNVVGLRPSLGRVPADGPFGWFARLLTPGPM
                     ARNVPDAALLLSVQAGPNQDDPLSRPEPGRHFRDLPHADPATLKIAWSADLGFAPVDA
                     AMVRLLEAAAVVFEDLGCEVENACPDLRGGMSVFQTQRAASLAQTGRALDRSLPRWRE
                     HAKETMIWNVEQGYALTAEDLLRCEVERTAIYQRVGAFFAHHDALLLPSAQVPPFDKS
                     LDWVREIDGRAMETYIDWMTICCAVTVTDLPAISVPGGFTDDGLPLGLQIVGRPHGDL
                     DLLAIARLFEEATGHGQRRPPQSEHAG"
     gene            complement(2407..2589)
                     /locus_tag="AC16UZ_12650"
     CDS             complement(2407..2589)
                     /locus_tag="AC16UZ_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247577.1"
                     /translation="MYTVVDIASLQDYVLVEARRKAQEAGTVPIYRKFPVREVLDTLV
                     VDEEIVLFKPSLFPAL"
     gene            2756..>3039
                     /locus_tag="AC16UZ_12655"
     CDS             2756..>3039
                     /locus_tag="AC16UZ_12655"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR6247578.1"
                     /translation="MPESLRVMVVCAHPDDAEFHAGGLLVQLAAAGTDIHLLTLTDGS
                     AGHAELDRASLARRRAREAQAAARRLGARCTVWDTPDGELEPSLQLRGRL"
BASE COUNT          539 a         1063 c          945 g          492 t
ORIGIN      
        1 ccctcggggt cctgctccga gagcaccctt agttccacct gccccgccac cagtcgcgtc
       61 gacaccaccg gcagggaacg ccgcaaccct tcggcctcgt cgcggggaac cactttcgac
      121 cacagccgtc ccgcaagacg cccggtcagc tccttgggtt caccctgcac cagaatccgc
      181 ccgccgccca tgacggccat gttcgggcaa agctcggcaa cgtcctcgac gatgtgggtg
      241 gacagaatga ccaccacctc ctcgccgatt ctggccagca ggttatgaaa gcgccggcgc
      301 tctgcgggat cgagccccgc cgtgggctcg tcgacgatca ccagctgcgg atcgccgatg
      361 agcgcctggg cgatcccgaa gcgttggcgc atacccccgg aaaagctgtc gatgctcttc
      421 ttgcgcacgt cccagaggtt cgtctgcacc agcagctgct cgacgagcgc tttgcgagcg
      481 ccgcgaccgc caatgccctt gagcgctgcg atgtgatcga gcatcacttc cggagacgtg
      541 cgcggatacg cgccgaactc ctgcgggagg tagcccagca cgcgacgcag ggcgttggga
      601 tccgcgagca catcgacgtc gttgaagtga atggaaccgc tgtccgggct ctgcagcgtc
      661 gcgatggtgc gcatgagcgt ggacttgccg gcaccgttcg gcccgagcaa cccgaacatg
      721 cccttggcga tctccagatc cacgccgtcc agcgcccgca caccgttggg ataggtcttc
      781 gacaaagcgt ttatcttcag catgctttct ccttgcagag ctcgatcggt cgttcctcga
      841 cgcccgagaa ccaccgcgct cccggcgcgg cccgttccac caacagttcg accatgccgg
      901 ccaaccgcaa tgcgtgcgcg ccaccacctt gccggcgcgc gttggcgaat cgagaatacc
      961 tcaatgagcg aactggcgcg gcaagcggct cttgatactg ccgggatgat tcgacgggga
     1021 gacgcatccg ccgtggaagt catggacgca cactacgatc gcatcgaaat cctgaacccc
     1081 cagatcaatg cgctcgtcaa cgttctgccc agagaggcgg cactcgagct ggctcgccaa
     1141 gcggatcggg cgcgcagccg cggtgagccg tgcggtcctc tgcacggtct gcccatggcc
     1201 gtcaaggaca cccatgacgc cgcaggcttt ccgaccacct ggggatttgt cccctgggcc
     1261 gaacgcgtgg ctaggcacga tggagcggtg gccgcacgcc aacgggccgc aggcgcactt
     1321 ttcatcgcga aatccaacgt gcccgagttc gccctgggat cacacacgtt caataacctc
     1381 ttcggaacca cgagcaatcc ctacgacctg tcgcgcacgg ccggaggcag cagtggcggc
     1441 gccgccgcgg cgctggcaac ggggatgctt cccctggccg acgggtcgga catgggcgga
     1501 tcgctgcgca acccggccgg gtactgcaac gttgtcggcc ttaggccctc attggggcgc
     1561 gtcccggcgg atggcccgtt cggctggttt gctcggctgc tcacccccgg ccccatggct
     1621 cgtaacgtgc ccgacgcggc tctgctgctg tccgttcagg caggccccaa tcaggacgac
     1681 cccctgagcc ggccggagcc gggcagacac ttccgcgatc tgccccacgc cgatccggca
     1741 accctgaaga tcgcctggtc cgcagatctc ggctttgcac ccgtggacgc cgccatggtc
     1801 cggctgctcg aggcagcggc ggtcgttttc gaagacctcg gctgcgaggt ggagaatgcc
     1861 tgtccggacc tgcgcggagg catgtcggta ttccagaccc agcgcgcggc cagcctggcc
     1921 cagaccggcc gggccctgga tcgctcgcta ccgcgctggc gcgaacatgc caaggaaacc
     1981 atgatctgga acgtggagca gggctacgcg ctcaccgcag aggatctgtt gcgctgcgag
     2041 gtcgaacgta cggcgattta ccagcgcgtc ggcgctttct ttgcccacca cgacgcgctg
     2101 ctgctgcctt ctgcccaggt accaccgttc gacaagtcgc tggattgggt gcgcgagatc
     2161 gatggccggg ccatggagac gtacatcgac tggatgacca tctgctgcgc cgtgaccgtc
     2221 accgatctcc ccgcgatctc agtccccggc gggttcacgg acgacggcct gcccctgggc
     2281 ctgcagatcg tcgggcgacc tcacggcgac ctcgatctgc tggccatcgc ccggctgttc
     2341 gaggaggcta ccggccatgg ccagcggcgt cccccgcaga gcgagcatgc cggctgaaaa
     2401 acgaccctag agcgcgggaa acaggctcgg cttgaagagg acgatctcct cgtcgaccac
     2461 gagcgtgtcc agaacctccc ggacgggaaa tttgcggtag atggggacgg tgccggcttc
     2521 ctgagctttc cggcgagcct ccacgagcac gtagtcctgc aacgaggcga tgtccaccac
     2581 ggtgtacacg tcctcatcgg cgcaaaaatc gcgaccgacg agacagcgaa agtcgcttct
     2641 caaaaccaac gaacaacggc ccgaagcggg tccaaccccc aacacccaca gtctatccac
     2701 gccgtaaatg gttgatgtaa aaaggatgta agagatgtaa cggagacctg acgccatgcc
     2761 ggaatcgtta cgagtcatgg tcgtctgcgc ccatcccgac gatgcggagt ttcacgccgg
     2821 cgggctgctc gtccagctgg ccgcagcggg cacggacatc cacctgctca ccctcacgga
     2881 cggctccgcc ggtcacgccg agctggatcg cgcctcgctc gcccgacgac gggccaggga
     2941 agcccaggcc gccgctcgcc ggctcggcgc gcggtgcacc gtctgggata cgccagacgg
     3001 cgagctcgag ccgagcctcc agctgcgcgg gcgactgat
//
LOCUS       DBNHOX010000577         3900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1140096, whole genome shotgun sequence.
ACCESSION   DBNHOX010000577 DBNHOX010000000
VERSION     DBNHOX010000577.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1140096"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="AC16UZ_12660"
     CDS             complement(<1..417)
                     /locus_tag="AC16UZ_12660"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6247579.1"
                     /translation="MDELFETYDEAGRAMGLMARHRVHATGAWHRSAHVFLFDTTGAL
                     LIQRRAPGKDLYPDLWDYSVGEHLVPGETYLAAARRGLEEELGVRGVSLEPIGDVRRW
                     RSHQAELNLRDNELQQAFTGVHDGPFEPDAAEVAEVR"
     gene            524..1504
                     /locus_tag="AC16UZ_12665"
     CDS             524..1504
                     /locus_tag="AC16UZ_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6247580.1"
                     /translation="MLKLQGIRLAEGAAPLDHEFRSGQITVVLGRNRSGKTRLCRLLA
                     GLEPTGEGRILLDGVDVSNIPAGQRPVSFVYQSFVNYPTLTVAENIASPMRARGLPSR
                     ERRRRVEDLAARLHISELLDRRPAQLSGGQQQRLAIARALAKQARVLVMDEPLVNLDF
                     KLREALELELRGLLRDAGIAVVYTSSDPRDAFNLGDEVLLLADHAPVQAGRPLDVYER
                     PVSVAAADLMSDPGVNLLPGPDRPAAIRPEHLHVSRHARDDETFAAELLTLETNGSET
                     YLHCRVADAHWVVKLEGLIDLKLRPGDTLPLFAEQRSLLRFSEAMTDVQD"
     gene            1491..2597
                     /locus_tag="AC16UZ_12670"
     CDS             1491..2597
                     /locus_tag="AC16UZ_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6247581.1"
                     /translation="MSRIELDGVSHRYPEAPSETFAELELVIGSGEAHALLGSSGAGK
                     TTLLNLLSGIEQPTTGRITFDGRDVSRTPPRARNVAQVFQFPVLYESLSVAENLEFPL
                     KTRGVRAAERRRRAKAMAERLGIAELMGRRPGKLSLFERQLVAIGRALVRPELSLVLL
                     DEPLTAVEPSIKWQLRRELKAVQQEIGATMIYVTHDQTEALTFADRISLLHRGRILQT
                     GSARTLFSAPAHEQVGHFIGSPGMNLVPGEIAEGALRLGGRVVAPAAGAEPGPCTIGF
                     RPEWASVAPSGPDGSGLLGTITAARVLGVRRGVPVGILSVDLGVGVVQVRQALSASPG
                     ERVGVNVDAGQVVAFREGWRLPGIGAETGRGAGS"
     gene            2594..3460
                     /locus_tag="AC16UZ_12675"
     CDS             2594..3460
                     /locus_tag="AC16UZ_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6247582.1"
                     /translation="MSGRRPDNRAWLGVLPVVLVVAFSAVIPLMTVVNYSLQDIFDQH
                     TRLFVGFDWYREILRDPRFLEALLRQLAFSGAVLLIEVPLGVMVARSLPRSGWLAPAL
                     VVLIALPLLVPWNVVGTIWQIFARPDIGLLGAALTGLGFEFNYTSSPVDAWITLLLMD
                     VWHWTSLVALLAYAGLASIQEAHYQAAEIDGAGRWQVFRLVELPRLRPVLTIAVLLRL
                     MDSFMIYTEPFVLTGGGPGNATTFLSQYLTSMAVGQFDLGPAAAFSVLYFLVTLLMCW
                     LFYTSVIRKEDE"
     gene            3457..>3900
                     /locus_tag="AC16UZ_12680"
     CDS             3457..>3900
                     /locus_tag="AC16UZ_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6247583.1"
                     /translation="MTRRSHWLIFIYCAYLLLPVYWLVNMALRSNRDIVGGLEIVPGR
                     PTLANFAEIVTNPVWYEGFANSLTYVGVNTVISLLVALPAAFAFSRFRFLGDRHLFFW
                     LLSNRMAPPAVFLLPFFQLYSSVGLFDTPWAVALAHTLFNVPLAVW"
BASE COUNT          541 a         1299 c         1323 g          737 t
ORIGIN      
        1 gcggacctcg gcgacctcgg cggcatccgg ctcgaagggg ccgtcgtgca cgccggtgaa
       61 cgcctgctgg agctcgttgt cccgcaagtt cagctccgcc tgatgtgacc gccaccgccg
      121 cacgtcgcca atcggctcga gcgaaacgcc ccttacgccg agctcctcct ctaggccgcg
      181 ccgggcggcg gccagatatg tctcgccggg caccaggtgc tcgccgacgc tgtagtccca
      241 cagatccgga tagaggtcct tgcccggcgc gcggcgttgg atgagcagcg cgccggtggt
      301 gtcgaaaaga aagacgtggg cagagcggtg ccaggcaccg gtagcgtgga cccgatggcg
      361 tgccatgagc cccatggccc tgcccgcctc gtcataggtt tcgaacagct cgtccaatgg
      421 tccttcgatt ctgctgggga tggcggacgc tatactttgg cctttgcacg tcgaggtcaa
      481 tgatgtccca gcgacgcagg cggccggcgg gtccaggaca gcggtgctga agctgcaggg
      541 aatccgtctg gccgagggcg ccgcgcccct ggaccatgag ttccggtcgg ggcagattac
      601 cgtggtcctc ggccgcaacc gctcgggcaa gacgcgcctg tgccgattgc tggccggcct
      661 ggagcccacg ggtgagggcc ggatattgct ggatggggtc gacgtctcga atatcccggc
      721 gggccaacgg ccggtctctt tcgtgtatca gtcgttcgtc aattacccca ccctcaccgt
      781 ggcggagaac atcgcctcgc ccatgcgggc ccggggcttg ccgtcgcgtg agcgtcgccg
      841 gcgggtcgag gacctggcgg caaggctgca catttcggag ctgctcgacc ggcgcccggc
      901 ccagctgtcc ggtggccagc agcagcgtct ggcgatcgcc cgggcgctgg ccaaacaggc
      961 gcgcgtcctg gtcatggacg agccgctggt gaaccttgat ttcaagcttc gtgaggcgtt
     1021 ggagctggag ctgcgcgggc tgctccggga tgccggtatc gccgtggtct atacgagctc
     1081 ggacccccgg gacgctttca acctgggcga cgaggtgctc ctgcttgccg accacgcacc
     1141 cgtgcaggcc ggccggccgc tggacgtcta cgagcgtccg gtcagcgtcg ctgccgcgga
     1201 cctgatgagc gaccccggtg tcaatctgct gcccgggcct gaccggccgg cggcgattcg
     1261 tcccgagcat ctgcacgtga gccgccacgc aagggatgac gagacgtttg ctgccgagct
     1321 cctcaccctc gagaccaacg ggtcggagac ctatctccac tgccgggtgg cggacgcgca
     1381 ctgggtggtg aagctggagg gcctgatcga tctgaagctc cggccggggg atacgctgcc
     1441 gcttttcgcc gagcagcgct ccctgctgcg attctcggag gcgatgaccg atgtccagga
     1501 ttgagctgga cggcgtcagc caccgatatc cggaggcgcc cagcgaaacg tttgccgagc
     1561 tcgagctggt catcggctcc ggcgaagccc acgcgctgct gggcagctcg ggcgcgggca
     1621 agacgacgct gctcaacctc ctctccggca tcgagcagcc gacgaccggg cgtatcacct
     1681 tcgatggccg ggacgtctcg cgcaccccgc cgcgcgctcg caacgtggcc caggtgttcc
     1741 agtttccggt gctttacgaa tctctcagcg tggcggagaa cctggaattt cccctgaaaa
     1801 cccgtggcgt gcgcgccgcc gagcggcgtc gccgcgcgaa agccatggcg gagcgcctgg
     1861 gcatcgcgga gctgatgggc cgccggcccg gcaagctgtc cctgttcgaa aggcaactcg
     1921 tggccatcgg tcgcgcgctg gtcaggcccg agttgtccct cgtgctgctc gacgagccgc
     1981 tgacggcggt ggagccttcg atcaagtggc agctcaggcg ggagctgaag gccgtgcagc
     2041 aggagatcgg cgccaccatg atctacgtga ctcacgacca gaccgaggcg cttacctttg
     2101 ccgaccgcat cagccttctg caccgcgggc ggatcctgca gaccggctcg gcccgtaccc
     2161 tgttcagcgc gcccgctcac gagcaggtgg gacacttcat cggctcgccg gggatgaacc
     2221 tcgttcccgg cgagatcgcc gagggcgcgt tgcggctcgg agggcgcgtg gtggccccgg
     2281 cggcgggtgc ggagcctgga ccgtgcacca tcggcttccg cccggagtgg gcatcggtgg
     2341 cgccgtccgg gcctgacgga tcgggcctgc tcgggacgat caccgcggcc cgggtgctcg
     2401 gcgtgcgacg tggggtgccc gtcgggatcc tcagcgtcga cctcggcgta ggcgttgtgc
     2461 aggttcgcca ggctttgtcc gcttccccgg gggagcgtgt gggtgtgaac gtggacgccg
     2521 ggcaggtcgt cgccttccgg gaaggctggc gcctcccggg aatcggtgcc gagaccggac
     2581 ggggggcagg ttcgtgagcg gacgtcggcc cgacaaccgg gcctggctcg gcgttctccc
     2641 ggtggtgctg gtggtcgcgt tcagcgccgt gattcccctc atgacggtgg tcaactactc
     2701 ccttcaggac atcttcgatc agcacacgcg tctgttcgtc ggcttcgact ggtatcgaga
     2761 gatccttcgc gacccgaggt tcctcgaggc gctgttgcga caacttgcgt tcagcggcgc
     2821 ggtgctcctc atcgaggttc cgctcggggt catggtggca cgcagccttc cgcgcagcgg
     2881 ctggctcgcg ccggcgctcg tcgtcctgat cgctttgccg ctgctggtcc cttggaacgt
     2941 cgtcggaacc atctggcaga tctttgcgcg cccggacatc ggcctgctcg gcgccgcgct
     3001 gaccggcctg ggtttcgagt tcaactacac gtccagcccc gtcgacgcct ggatcacgct
     3061 tctgctcatg gacgtctggc actggacgtc cctggtggcg ctcctggcct atgccggtct
     3121 tgcgtccatc caggaggccc attatcaggc ggccgagata gatggggccg gccgctggca
     3181 ggtcttccgg ctggtcgagc tgccgaggct taggcccgtt ctgaccattg ccgtgctgct
     3241 gcggctgatg gacagcttca tgatctacac ggagcctttc gtgctgaccg gcggcggccc
     3301 ggggaacgcc accactttcc tcagccagta cctgaccagc atggcggtag gccagttcga
     3361 tctggggccg gcggccgctt tttcggttct ctacttcctc gtcaccctgc tgatgtgctg
     3421 gctgttctat accagcgtga tcaggaagga agacgaatga cccgccgcag ccactggttg
     3481 atcttcatat actgcgcgta tcttctgctg cccgtttact ggcttgtcaa catggcgctg
     3541 cgcagcaacc gggacatcgt gggcgggctg gagatcgttc ccggccggcc tacgctggcc
     3601 aacttcgcgg agatcgtgac caacccggtc tggtacgaag ggtttgccaa ctcgcttacg
     3661 tacgtcgggg tgaacacggt gatttctctc ttggtggcgc tgccagccgc attcgctttt
     3721 tcgcgcttcc ggtttctggg cgacaggcat cttttcttct ggctgctctc gaaccgcatg
     3781 gcgccgcccg ccgtgttcct gctgccgttc ttccagctct actccagcgt cggcctgttc
     3841 gacacgccgt gggcggtcgc cctcgcccac accctgttca acgtgcccct cgcggtctgg
//
LOCUS       DBNHOX010000578         1655 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1203336, whole genome shotgun sequence.
ACCESSION   DBNHOX010000578 DBNHOX010000000
VERSION     DBNHOX010000578.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1203336"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="AC16UZ_12685"
     CDS             complement(<1..337)
                     /locus_tag="AC16UZ_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870004.1"
                     /note="Sms; stabilizes the strand-invasion intermediate
                     during the DNA repair; involved in recombination of donor
                     DNA and plays an important role in DNA damage repair after
                     exposure to mutagenic agents; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR6247584.1"
                     /translation="MAGKRKAKSAYVCSDCGAEHTQWQGQCVACSAWNTLTRVSIGPI
                     KEPVSRGYAGSQSQVSLLGEVEREEAERLPTGFAELDRVLGGGLVPGSVVLMGGDPGA
                     GKSTLLLQVA"
     gene            complement(337..>1655)
                     /gene="dnaB"
                     /locus_tag="AC16UZ_12690"
     CDS             complement(337..>1655)
                     /gene="dnaB"
                     /locus_tag="AC16UZ_12690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR6247585.1"
                     /translation="QSVLGALMLNDEAWFNVAEIVTARDFYRPQHQIIFEAMAELSAD
                     TQPLDAVTVSERLQSKGLLDKAGGNAYLGELAESIPGTSNVVAYAQIVREHSTLRQLI
                     SAANRIAETAFAPDGRPSGELLNMAEQEVFRIAEGRLRDSGPEKVVPLLHRAVERIEK
                     LYETRNPITGVATGFDDLDKKTAGLQPSDLVIIAARPSMGKTALAVNIVEHAVMEGSG
                     AVLMFSLEMPSEQIVMRMLSSLGRIDQTRMRTGDMHEDDWPRFTSAVSQLKDKPLYLD
                     DTPALTPNDIRTRARRVARESGGLGLVVVDYLQLMRGSGPVENRTNEISEISRSLKAI
                     AKELRCPVVALSQLNRGLESRTDKRPVMADLRESGAIEQDADVILFIYRDEVYNPESE
                     QKGTAEIIIGKQRNGPIGMCRLSYIGNLTKFENLAPDRYNDFQPFD"
BASE COUNT          263 a          545 c          537 g          310 t
ORIGIN      
        1 tggcaacctg cagcagcagc gtgctctttc ccgcgccggg atctccaccc atcagcacga
       61 cggagccggg caccaggccg ccgccgagca cccggtcgag ctcggcaaat ccggtgggca
      121 aacgctcggc ctcctcgcgc tccacctcgc cgagcaggct aacctggctc tggctgccgg
      181 cgtagccccg ggaaacgggt tctttgatgg gtccgatgga aacccgggtg agggtgttcc
      241 aggcgctgca ggccacgcac tggccctgcc actgggtatg ctcggcgccg cagtccgagc
      301 agacgtacgc ggatttggct ttgcgcttgc ccgccatcag tcaaatggct gaaagtcgtt
      361 gtagcgatcc ggcgccaggt tttcgaactt ggtcaggttg ccgatatagg acagacggca
      421 catcccgatg gggccgttgc gctgcttgcc gatgatgatc tcggcggtgc ccttctgctc
      481 ggattcgggg ttgtaaacct cgtcgcggta gatgaaaagg atcacgtccg cgtcctgctc
      541 gatggcgccc gactctctaa gatcggccat gaccggccgc ttgtcggtac ggctctccag
      601 cccccgattg agctgggaaa gcgcgaccac cgggcagcgc agctccttgg cgatggcctt
      661 cagagatcgg gagatctccg aaatctcgtt cgtgcggttc tccacaggtc cggaaccgcg
      721 catgagctgc aggtagtcga ccaccaccag gccgaggccg ccggattcgc gggctacccg
      781 ccgcgcccgg gtgcggatgt cgttcggcgt cagcgcaggc gtatcgtcca ggtacagagg
      841 tttgtctttc agctggctga cggcggaggt gaaccgcggc cagtcgtctt cgtgcatgtc
      901 cccggtgcgc atgcgggtct gatcaatcct gcccagcgat gagagcatgc gcatgacgat
      961 ctgctcagac ggcatctcca ggctgaacat gagcacggcg ccgctgcctt ccatcaccgc
     1021 gtgctcgacg atgttgaccg caagggccgt tttgcccatg gacggccgcg cggcgatgat
     1081 caccagatcg gacggctgca ggccggccgt cttcttgtcc agatcgtcga agccggtggc
     1141 cacgccggta atggggttgc gggtctcgta gagcttctcg atgcgctcca ccgcccggtg
     1201 cagcagcggg acgacctttt cgggtccact gtcgcgcaga cgcccttcgg cgatgcggaa
     1261 gacctcctgc tcggccatgt tcagcagctc cccgctgggc ctgccgtcgg gagcgaacgc
     1321 cgtttccgca atgcggttgg ccgcgctgat cagctgacgc aacgtggagt gctcgcgcac
     1381 gatctgcgcg taggccacca cgttgctcgt gccgggaatc gactcggcca gctcgcccag
     1441 gtaagcgttg ccgcccgcct tgtcgagcag gcccttggac tgcagccgct ccgagacggt
     1501 caccgcgtcc agaggctgcg tgtctgccga cagctccgcc atggcttcga agatgatctg
     1561 atgctgcggc cggtagaagt cccgggcggt gacgatctcg gcaacgttga accacgcttc
     1621 gtcgttcagc atcagcgcgc ccagcacgga ctgct
//
LOCUS       DBNHOX010000579         1653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1267048, whole genome shotgun sequence.
ACCESSION   DBNHOX010000579 DBNHOX010000000
VERSION     DBNHOX010000579.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1267048"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="AC16UZ_12695"
     CDS             complement(<1..1354)
                     /locus_tag="AC16UZ_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005754175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HIR6247586.1"
                     /translation="MTDEAKCPVLNHDHSARGSQANQHWWPNQLNLNILHQGSAKADP
                     MGEEFDYAAEFATLDLAAVKADLTALMTDSKEWWPADYGHYGPFFIRMAWHSAGTYRT
                     HDGRGGGASGSQRFAPLNSWPDNGNLDKARALLEPIKKKYGRKISWADLMILTGNVAL
                     ESMGFETFGFAGGREDVWEPEQDIYWGKETEWLGDRRYSGDRELENPLAAVQMGLIYV
                     NPEGPNGKPDPLAAARDIRETFARMAMNDEETVALIAGGHTFGKCHGAGDPALVGAEP
                     EGASIEEQGLGWRSSFGSGRGGDAITSGLEGAWTPNPVRWDSGYFDTLFGYDWELTKS
                     PAGAWQWTPTDPSAADVVPDAHDPAKRHAPMMATTDLSLREDPIYEPIARRFHESPDE
                     LADAFARAWYKLTHRDMGPRARYLGPLVPAEELLWQDPVPEVDHELVDAGDVASLKGR
                     I"
     gene            complement(1430..>1653)
                     /locus_tag="AC16UZ_12700"
     CDS             complement(1430..>1653)
                     /locus_tag="AC16UZ_12700"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR6247587.1"
                     /translation="GLIRRIQPSGSVARYESRVGDNHHHLVCRGCGLIFDIDCAVGST
                     PCLTADDDHGFEIDEAEVAYWGRCPDCRA"
BASE COUNT          231 a          549 c          568 g          305 t
ORIGIN      
        1 gaatcctgcc cttgaggctt gcgacgtccc cggcgtcgac cagctcgtgg tcgacctccg
       61 ggacggggtc ctgccacagg agctcctcgg cggggaccag cgggccgagg tagcgggcgc
      121 gggggcccat gtcgcggtgg gtcagcttgt accacgcccg agcgaacgca tcggccagct
      181 cgtccgggct ctcgtggaag cgacgcgcga tcggctcgta gatcgggtcc tccctgagcg
      241 agaggtcggt cgtcgccatc atgggcgcgt gcctcttcgc agggtcgtgc gcgtccggca
      301 cgacgtcggc ggccgaaggg tccgtcggag tccactgcca ggctccggcc ggactcttgg
      361 tcagctccca atcgtagccg aacagggtgt cgaagtagcc gctgtcccat cgcaccgggt
      421 tgggcgtcca ggcaccctcc aggccgctgg tgatcgcgtc gcctcccctg ccgctgccga
      481 agctgctcct ccagcccagc ccctgctcct cgatgctcgc gccctcgggc tcggcgccca
      541 cgagcgccgg gtcacccgcg ccgtggcact tgccgaaggt atgcccgccg gcgatgagcg
      601 cgacggtctc ttcgtcgttc atcgccatcc gcgcgaacgt ctctcgaatg tccctggccg
      661 cggccagcgg atccggcttt ccgttcgggc cttccgggtt cacgtagatg aggcccatct
      721 gcacggcggc gagcgggttc tccagctccc ggtccccgga gtagcgcctg tccccgagcc
      781 actcggtctc cttgccccaa tagatgtcct gctcgggctc ccagacgtcc tcgcgtcccc
      841 cggcgaagcc gaacgtctcg aagcccatcg actcgagggc gacgttgccg gtgaggatca
      901 tcaggtcggc ccaggagatc ttgcggccgt acttcttctt gatcggctcg agcagcgcac
      961 gcgccttgtc gaggttgccg ttgtcgggcc agctgttcag cggggcgaac cgctggctgc
     1021 ccgaagcgcc gccgccacga ccgtcgtggg tgcggtaggt gccggcagag tgccaggcca
     1081 tccgaatgaa gaacgggccg tagtggccgt agtcggccgg ccaccactct ttggagtcgg
     1141 tcatgagggc ggtgagatcg gccttgacgg cggccagatc gagcgtggcg aactcggctg
     1201 cgtaatcgaa ctcctcaccc atcggatcgg ccttggccga accctgatgg aggatgttca
     1261 agttgagctg gttcggccac cagtgctggt tggcctggct gccacgcgcc gaatggtcgt
     1321 ggttgagaac cgggcacttt gcttcgtcgg tcatgttgtt ctgtgactcc tgcgggtctg
     1381 gttggttgct ggttggggta ggaacgtggt tcgtgatcag gcgtcggggt caggcccgac
     1441 aatcggggca tcggccccag taggcgactt cggcttcgtc gatctcgaag ccatggtcgt
     1501 cgtcagcggt gagacagggg gtcgacccga cggcacagtc gatgtcgaag atcagaccgc
     1561 agcctcgaca caccaggtgg tggtggttgt cgcccacacg gctctcgtag cgcgcaaccg
     1621 agccggaggg ctggatccga cggatgagcc cct
//
LOCUS       DBNHOX010000580         3831 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1267072, whole genome shotgun sequence.
ACCESSION   DBNHOX010000580 DBNHOX010000000
VERSION     DBNHOX010000580.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1267072"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..310
                     /gene="rpmI"
                     /locus_tag="AC16UZ_12705"
     CDS             110..310
                     /gene="rpmI"
                     /locus_tag="AC16UZ_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR6247588.1"
                     /translation="MPKMKTKSGAAKRFRRTANGFKHRKANRNHILTKKAPKRKRQLR
                     GLHDVSAADAPAINRMIPKKAR"
     gene            320..685
                     /gene="rplT"
                     /locus_tag="AC16UZ_12710"
     CDS             320..685
                     /gene="rplT"
                     /locus_tag="AC16UZ_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR6247589.1"
                     /translation="MPRARNTVASRTRRKKVLKQAKGYYGARSRSFKVAKQAVIKAGQ
                     YAYRDRRQRKRQFRSLWVVRINAAAREHDLSYSRFIAGLKKAAIEVDRKVLADLAVHE
                     KETFAVLAEQAKQALAEAA"
     gene            815..1828
                     /gene="pheS"
                     /locus_tag="AC16UZ_12715"
     CDS             815..1828
                     /gene="pheS"
                     /locus_tag="AC16UZ_12715"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010209123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR6247590.1"
                     /translation="MNIEELLEQARTGIAEAGDEAALDAVRVRFLGKKGELTVLLKSL
                     GTLDAEERPKAGAAINLAKEQVQEWIAQRRASLGSEQLAQALASESVDVTLPGRGERP
                     GGLHPVTQAMERIEDIFTGAGYSVVPGQEIEDDYHNFEALNIPAHHPARAMHDTFYFG
                     NGLLLRTHTSPSQVHVMENQAPPIRVICPGRVYRRDSDLTHTPMFHQVEGLVIDRGIS
                     FADLKGTVVGFLQRFFEQDLAVRFRPSYFPFTEPSAEVDVQCVHCLGKGCRVCSQTGW
                     LEVMGCGLVHPNVLEMSGVDSEEFSGFAFGMGGDRLAMLLYEVDDLRMFFDNDLRFLR
                     QFT"
     gene            1847..>3831
                     /gene="pheT"
                     /locus_tag="AC16UZ_12720"
     CDS             1847..>3831
                     /gene="pheT"
                     /locus_tag="AC16UZ_12720"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR6247591.1"
                     /translation="MKFSEAWLREWADPGISREALLDQLTMAGLEVDGVEPVSKEFAG
                     VVIAEVVSVDAHPNADKLSVCTVDDGTQTFQVVCGAPNVRAGMIGAFARVGAVLPDDF
                     RIKAAKLRGVESQGMLCSAAELGMGDDHSGILDLDCPDRVGDDLRQALALDDVIVDLD
                     LTPNRGDCLSVRGLAREVGVLNNVPVAAGEVEAVAPVLEDTFSVEIQDPAGCPRYLGR
                     VIRGIDPSCASPLWLTEKLRRCGVRAIDPVVDVTNFVMLELGQPMHAFDLGQLRDGIV
                     VRRARDGEQLRLLDGQELALDADTLLITDGSGPVAIAGVMGGERSGIQAATTDVFLEC
                     AYFAPLAVAGTARRYALHTDASHRYERGVDFELQHAAMERATRLLLDIVGGEPGPVVE
                     AVEPKALPEVSDIGLRERRLNRLLGTEIAPEIVDEAMERLDFAVTSRETGEEGVRWTV
                     RVPSHRFDIVREADLVEEVCRIYGYNNLPVRQPVTELELTPVPLERSPAGRLKRLLAT
                     LGYQEAITYSFVDPRLQDLLAPAADALALSNPMSSEQSVMRLNLLPGLVEALRFNVAR
                     QQDRVRIFELGSCFAPGDPVLQKLMLGGVLWGRRHGENWNQSGAEVDFYDLKGDVEAI
                     VGWSGEPVAYERLEDPVLHPGQAALVVGSRGPVGRLGR"
BASE COUNT          690 a         1168 c         1325 g          648 t
ORIGIN      
        1 tggaagccga gccgaagttc gaggggcggc aggtggtgat ggtcttgggt cccaaaaaga
       61 agaagaaata gaacagcatc atacaagtct ggaaattgcg agtaagttca tgccgaaaat
      121 gaagacgaaa agcggcgcgg cgaaacggtt tcgccgcact gcaaatggtt tcaagcaccg
      181 caaggcgaat cggaaccaca tattgaccaa gaaggcgccg aagcggaaac gccagcttcg
      241 cggccttcac gacgtatctg cggctgatgc cccggcgatc aatcgcatga ttcccaaaaa
      301 ggcgcgatag gaggacggga tgcccagagc aagaaatacc gtcgcctcgc ggacgcgccg
      361 gaaaaaggtg ctcaagcagg ccaagggcta ttacggagcg cgcagccggt ccttcaaggt
      421 cgccaagcag gcggtcatca aggccggaca gtacgcctac cgcgatcgcc gtcaacgcaa
      481 gcgccagttc cgttcgttgt gggttgtcag aatcaacgcc gcggcacgcg agcacgacct
      541 ttcctacagc cgatttatcg ccggcctcaa gaaggcggca atcgaagtgg atcgaaaggt
      601 gctcgccgat ctggccgtac acgagaagga aaccttcgcc gtgctggccg agcaggccaa
      661 gcaggctctc gcggaagcag cctgaagcac tgatcggtaa acatcagccg tcttctgggg
      721 gccgtcgaag ggctcttccc ggaagcggac gcgtacgagc gaatctaggg aaggagccgg
      781 cagcggtact tccctttttt cgtttgagac cgagatgaac atagaagagc tactcgagca
      841 ggccaggacg ggaatcgccg aagcagggga tgaagcggct ctcgacgccg tgcgcgttcg
      901 ctttctcggc aagaaggggg aactcaccgt tctcctgaag tccctgggta ctctcgatgc
      961 cgaggaacga cccaaggctg gcgcggccat caatcttgcg aaggaacagg ttcaggagtg
     1021 gatcgcgcag cgccgcgcgt cgctcggctc cgagcagctt gcccaggcgc tcgccagcga
     1081 aagcgttgac gtgaccctgc ccggtagggg agagcggcct ggaggcctgc atccggttac
     1141 ccaggcgatg gagcggatcg aggacatctt caccggagcc ggctacagcg tcgtcccggg
     1201 ccaggagatc gaggacgact atcacaattt cgaggctctc aacattcccg cgcaccatcc
     1261 ggcccgggcc atgcacgaca ccttctactt cggcaacggc ctgctgctga ggacccacac
     1321 ctcgccgtct caggttcacg tcatggagaa ccaggcgccg cccatccggg tcatctgccc
     1381 ggggcgcgtc tatcgacggg attccgatct cacccatacc cccatgtttc accaggtcga
     1441 agggctggtc atcgatcgcg gcatcagctt tgccgatcta aagggcacgg tggtgggctt
     1501 tctgcagcgg tttttcgagc aggatctcgc cgtccggttc cggccttcgt acttcccgtt
     1561 caccgagccg tctgccgaag tggacgtcca atgcgttcac tgcctgggca agggctgccg
     1621 ggtctgcagc cagaccggct ggctcgaggt gatgggctgc gggctcgtcc acccgaacgt
     1681 gctcgaaatg tcgggcgtgg acagcgagga gttctcgggg ttcgccttcg gcatgggcgg
     1741 cgaccggctc gccatgctgc tctacgaggt cgacgacctg cgcatgttct tcgacaacga
     1801 tctccgcttc ctgcggcagt tcacgtgacc gggtcggggg gcgatcatga agttcagtga
     1861 agcctggctg cgcgagtggg cggacccagg catctccagg gaggcgctgc tggaccagct
     1921 gaccatggcc gggttggagg tggacggcgt tgagcccgta tccaaagagt ttgccggcgt
     1981 cgtgatagct gaggtggtct cggtggacgc ccaccccaat gcggacaagc tttcggtttg
     2041 caccgtcgac gatggcacac agaccttcca ggtggtgtgc ggcgcgccaa acgtccgggc
     2101 gggcatgatc ggcgccttcg cccgggtggg ggccgtgctt ccggatgatt tccgcatcaa
     2161 ggcggccaag cttcgcggag tcgagtccca gggcatgctc tgcagcgcgg ccgagctcgg
     2221 catgggcgat gaccacagcg gcatcctcga cctcgactgc cccgaccgcg tgggcgatga
     2281 cctgcgacag gcgctggccc tcgacgacgt catcgtcgac ctggacctca cgccgaaccg
     2341 cggtgactgc ctgagcgtgc gaggtctcgc ccgggaagtg ggggtgctca acaacgttcc
     2401 ggtcgccgcc ggcgaggtgg aggccgtcgc gcccgtgttg gaggatacgt tcagcgtcga
     2461 gatccaggat cccgcaggct gcccgcgata tctgggacgg gtaattcggg gcattgatcc
     2521 ctcctgcgcg agcccgctgt ggctcaccga gaagctgcgc cgctgcggcg tgcgcgccat
     2581 cgacccggtg gtggacgtca ccaatttcgt catgctcgag ctgggtcagc ccatgcatgc
     2641 cttcgacctc gggcagcttc gcgacggcat cgtggtccgg cgggcgcggg atggcgagca
     2701 gctgaggttg ctggatggcc aggaactggc cctggacgcg gacaccctgc tgatcacgga
     2761 tggcagcgga ccggtcgcca tcgccggggt gatgggcggg gagcgcagcg gcattcaggc
     2821 ggcaaccacc gacgtgttcc tggagtgcgc ctacttcgcc ccgctggcgg tagccggcac
     2881 ggcgcgtcgg tacgcgctcc acacggacgc gtcgcaccgc tatgagcggg gcgtggattt
     2941 cgagctgcag catgcggcca tggagagagc gacccggctg ctcctggaca tcgttggcgg
     3001 ggagccggga ccggtggtgg aggcggtgga accgaaagcc cttccggagg tgtccgacat
     3061 cgggttgcgc gagcgtcggc tcaaccgtct tctcgggacg gagatcgcgc cggagatcgt
     3121 cgacgaggcc atggagaggc tcgacttcgc ggtgacgtct agggagacgg gagaggaggg
     3181 cgttcgctgg actgtgcgcg tgcccagcca ccggttcgac atcgtgcgcg aagcggatct
     3241 cgtcgaagag gtctgccgca tctacggtta caacaacctg ccggtgcgcc agccggtgac
     3301 cgagctggag ctcacgccgg tgcccctcga aaggagcccg gcaggacggc tgaagcggct
     3361 gctggcaacc ctcggctatc aggaagccat cacctacagc ttcgtggacc cgcgtctgca
     3421 ggatctgctc gcgcccgccg ccgatgccct ggcgctgagc aatcccatgt cgtcggagca
     3481 gtcggtcatg cggctgaacc tgctgcccgg cctcgtcgag gcgctgcgtt tcaacgtggc
     3541 ccggcagcag gaccgggtgc ggatattcga gctggggtcg tgctttgccc ccggcgaccc
     3601 cgtgctgcag aagctgatgc ttggcggtgt gctctggggt cggcggcacg gggagaactg
     3661 gaaccagtca ggcgcggaag tcgacttcta cgatctcaag ggcgacgtcg aggccatcgt
     3721 ggggtggagc ggcgagccgg tggcctacga gcggctcgaa gacccggtgc ttcaccctgg
     3781 ccaggcggcg ctcgtggtgg gttcgcgagg gccggtcggg cggctggggc g
//
LOCUS       DBNHOX010000581         3768 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1140436, whole genome shotgun sequence.
ACCESSION   DBNHOX010000581 DBNHOX010000000
VERSION     DBNHOX010000581.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1140436"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /gene="dapF"
                     /locus_tag="AC16UZ_12725"
     CDS             complement(<1..434)
                     /gene="dapF"
                     /locus_tag="AC16UZ_12725"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR6247592.1"
                     /translation="MKLEFAKMHGLGNDFMVLDLISQDFEPNREQIHAWADRRTGIGF
                     DQMLTISAPTDPDADFRYRIYNADGTEVEQCGNGARCAARFIAERKLSPKDTLVLETL
                     AGPISTALLDAETVEVDLGVPSTRAEDVPFSPEAACGAQGDAV"
     gene            complement(431..1705)
                     /gene="lysA"
                     /locus_tag="AC16UZ_12730"
     CDS             complement(431..1705)
                     /gene="lysA"
                     /locus_tag="AC16UZ_12730"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR6247593.1"
                     /translation="MDAFSITDGVLHAENVPLPRIAEAVGTPTYVYSTAHLKAQYQRL
                     RRALGELDADVCYAVKANANLAILRLFAEQGAGFDIVSGGELQRVISAGGDPARVIFS
                     GVGKSVAEIDFALKLGIRCFNVESASELDCLSERARVLKRQAPMSIRVNPDVDAGTHP
                     YISTGLRSNKFGIPPGQAVELYRRAQADEYLSVHGIDCHIGSQIGTIDPLIDALRNLL
                     ELTDRLSAEGIRLEHVDVGGGLGIRYGDEEELDVEAYGAALCELLRGRELKLLLEPGR
                     YLVGNGGVLLTRTRFLKPTTAETEKNFAVVDAAMNDLIRPALYQAWHEVRPVTPRAGA
                     AELCWDVVGPVCETGDFLAERRELALQEGDLLAVMSAGAYGMVQSSNYNTRPRPAEVL
                     VAGDRFRVVRRRETTRDQLALELEAEHAETWR"
     gene            complement(1889..3268)
                     /gene="argH"
                     /locus_tag="AC16UZ_12735"
     CDS             complement(1889..3268)
                     /gene="argH"
                     /locus_tag="AC16UZ_12735"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR6247594.1"
                     /translation="MSRDSKPWGGRFSEATDAFVESFTASVAFDKRLYHHDIRGSIAH
                     ANMLSRIGVLTEGERNEIVSGLQAIHKEIDEGELAWRVELEDVHMNVESRLIELIGET
                     GKKLHTGRSRNDQVATDIRLYLKDAIDELVALETQLLGALVDLAEAHADTVMPGFTHL
                     QPAQPVTFGHHLLAWFEMLHRDRDRLNDCRRRVDVMPLGSAALAGTTYPIDRAGVAAE
                     LGFGAVAENSLDAVSDRDFAIEFTSVAALTMNHFSRWSEELVLWTSQQFGFVELPDRF
                     CTGSSIMPQKKNPDVPELIRGKSGRVAGNLVALLTLMKGQPLAYNKDNQEDKEPLFDT
                     VDTLRDCLTALNGLVPAITPNRERMRSAAAAGFTTATDLADYLVRRDVPFRDAHEVVG
                     RAVAHAIGQGKGLDELTIEELRAFSDSIGEDVFSVLTLEGSVSARNHPGGTAPDSVRE
                     AAARARDRL"
     gene            3294..>3768
                     /locus_tag="AC16UZ_12740"
     CDS             3294..>3768
                     /locus_tag="AC16UZ_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6247595.1"
                     /translation="MADYSNHRERFVVPELCNAAAISLLVLFGELLVLVLLFAGGPIT
                     WLRLALMSLFVQWVVIMSAGALCWTRAWLSRFAVVPGAVLAFSMVMAITLFVGISADR
                     IMAPPGVEAINWMGILGQLLISGIITSLALRFLYVQQQLRAQEQSELQSRIQALQS"
BASE COUNT          574 a         1267 c         1238 g          689 t
ORIGIN      
        1 accgcatcgc cctgcgcgcc gcacgccgct tccgggctga acggcacgtc ttcggcccgg
       61 gtgcttggaa cccccagatc gacctccacg gtctcggcgt ccagcagggc cgtggatatg
      121 ggccccgcca acgtctcgag caccagggtg tctttcgggc tcagctttcg ctcggcgatg
      181 aatctggccg cgcatcgggc gccgttgccg cactgctcca cctcggtgcc gtccgcgttg
      241 tagatgcggt agcggaagtc cgcatccggg tcggtgggcg ccgagatggt gagcatctga
      301 tcgaagccga tcccggtgcg gcgatcggcc caggcgtgaa tctgttcccg gttcggctcg
      361 aaatcctggc tgatcaggtc cagcaccatg aagtcgttac cgagcccgtg catcttcgcg
      421 aactcgagct tcatcgccac gtctccgcgt gctccgcctc gagctccagg gcgagctgat
      481 cgcgagtggt ttcgcggcgc cgcaccaccc ggaagcggtc tccggcgacc agaacctccg
      541 cgggtctggg acgcgtgttg tagttggagc tctgcaccat cccgtaggcc ccggcggaca
      601 tgacggccag aaggtcgcct tcctgaaggg ccagttctct gcgttcggcc aggaagtcgc
      661 cggtctcgca gacggggccc accacgtccc agcacagctc ggccgccccc gctcgcggcg
      721 tgacgggacg gacctcgtgc cacgcctggt acagcgccgg gcggatcaga tcgttcatgg
      781 cagcgtcgac caccgcgaag ttcttctcgg tctcggccgt cgttggtttc aggaatcgcg
      841 ttcgggtcag cagcacgccg ccgttcccca ccagatacct gccgggttcc agcaggagct
      901 tgagttcccg gccgcgcagc agctcgcaaa gggccgcacc gtatgcctcg acgtcgagct
      961 cctcctcgtc cccgtagcgg atgcccagcc cgccgcccac gtccacgtgt tccaggcgga
     1021 ttccctccgc ggacagccgg tcggtgagtt ccagcaggtt gcgaagggcg tcgatcagcg
     1081 ggtcgatggt gccgatctgg gagccgatgt ggcagtcgat cccgtgcacg gataggtact
     1141 cgtctgcctg cgcccgcctg tacagctcga ccgcctggcc cggcggtata ccgaacttgt
     1201 tgctgcgtaa ccccgtggag atgtagggat gcgtcccggc atccacgtcc gggttgaccc
     1261 ggatggacat gggcgcctgg cgcttgagca cccgtgcccg ctcgctcagg cagtcgagct
     1321 cgctggcgct ctccacgttg aagcagcgga tgccgagctt gagcgcgaag tcgatctcgg
     1381 ccaccgactt gcctaccccg gagaaaatca ccctggcagg gtcgcctcct gcgctgatga
     1441 cccgctgcag ctcgccgccg gagacgatgt cgaaaccggc gccctgctca gcgaagagcc
     1501 tcagaatggc cagattggcg ttggccttga ccgcgtagca gacgtccgcg tcgagctcgc
     1561 cgagcgcgcg gcgcagccgc tggtactgcg ccttcaggtg ggcggtcgag tagacgtacg
     1621 tcggcgtgcc gaccgcctcg gcaatccgcg gcagcggcac gttctccgcg tggagcaccc
     1681 cgtctgtgat ggagaaggcg tccacgtcag ggccggtggc tggcggcggg cgcttctgac
     1741 tgagcgtcgg gcagctccag gggacccttc tgaccgcaca tgaccgcctg agaggcaaga
     1801 atgattaaca ggatccagcg caccggtggc tcgcagctag gccgaacgag tcagtataca
     1861 ccgcctcatg gcggcgcagc ggccgcggtc aaagccggtc gcgggcgcgg gctgccgcct
     1921 cgcgcacgct gtcgggggcg gtgccgcccg ggtgattgcg cgcactgacg gacccctcga
     1981 gcgtgagcac ggaaaacacg tcctcgccta tgctgtcgct gaacgcgcgc agctcctcga
     2041 tcgtcagctc gtcgagaccc ttgccctgcc cgatcgcgtg cgccacggcg cggcccacca
     2101 cctcgtgggc gtcccggaag ggcacatcgc gccgcaccag gtaatcggcc aggtccgtcg
     2161 cggtggtaaa gcccgcggcg gcggccgaac gcatccgctc ccgattgggg gtgatggccg
     2221 ggacgaggcc gttcagagcc gtcaggcaat cgcgcagggt gtccaccgtg tcgaacagcg
     2281 gctccttgtc ctcctggttg tccttgttgt aggccagcgg ctggcctttc atcagcgtca
     2341 gcagagccac caggttgccg gccaccctgc ccgacttgcc ccggatcagc tcggggacgt
     2401 cggggttctt cttctgcggc atgatgctcg agcccgtgca gaaacgatcg ggcagctcga
     2461 cgaatccgaa ctgctgggac gtccacagca ccagctcctc gctccagcga gaaaagtggt
     2521 tcatcgtcaa cgcggcaacc gacgtgaact cgatggcgaa gtcccggtcg ctcaccgcgt
     2581 ccagcgagtt ctcggccacg gcgccgaatc cgagctcggc agcgacaccg gcccgatcga
     2641 tgggataggt ggtgccggca agcgccgcgc tgcccagggg catgacgtcc acgcgccggc
     2701 ggcagtcatt gaggcggtcg cggtcgcggt gaagcatctc gaaccacgcc agcagatggt
     2761 ggccgaacgt tacgggctgc gccggctgca gatgcgtgaa accgggcatg acggtgtccg
     2821 cgtgggcttc ggccaggtcc accagcgcgc cgagcagctg ggtttccaaa gcgaccagct
     2881 cgtcgatggc gtccttcagg tagagccgga tatcggtggc gacctggtcg ttgcgggacc
     2941 ggcccgtgtg cagctttttc ccggtttctc caatgagctc gataaggcgc gactccacgt
     3001 tcatgtggac gtcctcgagc tcaacccgcc aggcgagctc gccttcgtcg atctctttgt
     3061 gaatcgcctg caatcccgat acgatctcgt tgcgctcacc ttcggtgagc acgccgatcc
     3121 ggctcagcat gttggcgtgg gcgatggagc ccctgatatc gtgatggtaa agacgcttat
     3181 cgaatgccac cgaggccgtg aacgattcga cgaacgcatc ggtggcttcc gaaaagcggc
     3241 cgccccaggg ttttgaatct cggctcatgg tgacaagagg gttcctttag cggatggccg
     3301 attatagcaa ccatcgcgag cggttcgtgg tccccgagct ttgcaacgcg gcggccattt
     3361 ccctgctcgt gctcttcggt gagcttctgg ttctggtgct gctgttcgcc ggcggcccca
     3421 tcacgtggct ccggctcgcg ctcatgtccc tgttcgtgca gtgggttgtc atcatgagcg
     3481 cgggagccct gtgctggacg cgagcctggc tctcccggtt cgcggtggtg cctggcgccg
     3541 tgctcgcttt ctcgatggtc atggcgatca ccctgttcgt cgggatcagc gccgatcgga
     3601 tcatggctcc gcccggcgtg gaagcgatca actggatggg cattctcggc cagctcctca
     3661 tttccgggat catcacttcc ctggcgctgc gcttcctgta cgtccagcag cagctgcgcg
     3721 ctcaggagca gtcggagctc cagtcgcgca tccaggcgtt gcagtcgc
//
LOCUS       DBNHOX010000582         5863 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1267311, whole genome shotgun sequence.
ACCESSION   DBNHOX010000582 DBNHOX010000000
VERSION     DBNHOX010000582.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1267311"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="AC16UZ_12745"
     CDS             complement(<1..444)
                     /locus_tag="AC16UZ_12745"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR6247596.1"
                     /translation="MHRAVERKGGRAALDALLPAARSGSDLAALPDRDYLAEMTRCVF
                     RSGFVWKIIENKWPGFEVAFRGFDVAGCAMLSDEDIEQLSADERIVRHAAKIRSVPAN
                     AAFILDVRREAGGFGRYLADWPEDEIVGLWDELKRRGSRLGGQTGR"
     gene            500..877
                     /locus_tag="AC16UZ_12750"
     CDS             500..877
                     /locus_tag="AC16UZ_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cereblon family protein"
                     /protein_id="HIR6247597.1"
                     /translation="MVDVLDKTRQDLDPKLKEIVEKDERNFVYCGICSHVLTREVERI
                     EVSGSHDHHCTNPFGLRFHIGCFAEALGCDISGEPMAADTWFPGFRWRHASCAECHQH
                     LGWYFEAGERYFYGLILDRIQHD"
     gene            870..1559
                     /locus_tag="AC16UZ_12755"
     CDS             870..1559
                     /locus_tag="AC16UZ_12755"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR6247598.1"
                     /translation="MTDLAAGNYRTLTFDCYGTLIDWENGILGYLQPLLESYDVHVLD
                     EWVLEFFAENEPALQAEGGSYRSVLARLLERLGTRCAFTPNDDALKGFADSIEYWQPF
                     ADTNAALRALSEHFDLAVVSNVDNELFSLSARQLGVEFAQVITADDVGAYKPARAMFD
                     EALRKVSGPVLHVAQSLYHDIAPAKALGLDTVWINRPSAAGSGAARFVEVEPTWTFGD
                     LSELVEAFDLD"
     gene            1612..2124
                     /locus_tag="AC16UZ_12760"
     CDS             1612..2124
                     /locus_tag="AC16UZ_12760"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR6247599.1"
                     /translation="MSRPSLTSIQISKEYLHFNSAHFTLFSDREREDLHGHTFYVAGA
                     FDAEVGPDGLAFDYNLVKTKLKQLCDELDEKTLLPERSPHLRLSEENGYLVAEFAGER
                     MPFLPRDVLTLPVRNVTVEELAPFLLERLGSDPEIRTLELQRIELRVSSGPGQWAAAT
                     WTAGQEIREV"
     gene            2128..2823
                     /locus_tag="AC16UZ_12765"
     CDS             2128..2823
                     /locus_tag="AC16UZ_12765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6247600.1"
                     /translation="MKILLITGASAGIGLHTAQRFVAEGFAVVNLSRRRCPIDAVSQI
                     NCDLSVPGFIDNISNQLSGMLQEADRVVLVHNAARMGNDTAVETPSVALREALEVNVV
                     APNTLNYFTIPYMKPGSAIIYVGSTLAEKAVPGTYTYVVSKHALIGMMRSTCQDLAGQ
                     SIHTACVCPGFTDTEMLRQHVPAEAMASIRAMSAYGRLIDPDEIAETIFWAASNPVIN
                     GAVLHANLGQRES"
     gene            2823..3629
                     /locus_tag="AC16UZ_12770"
     CDS             2823..3629
                     /locus_tag="AC16UZ_12770"
                     /inference="COORDINATES: protein motif:HMM:NF014631.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160254 - tRNA queuosine(34) biosynthetic
                     process from salvaged queuosine or its precursors
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HIR6247601.1"
                     /translation="MQGGVRTAGGNEETTGNRLSEQDLNERRERVFIVLAGFFLCAMT
                     LLNVVGITRFIQLGPMALAVGVLPYPLTFLCTDLISELYGRKRANFLVWVGFTLNFFI
                     LGMLWLGSSIPAVDPATQPPWQVIELAEEIALPNGDVLSGSVELFQLLYACTSGAVFA
                     SMMAYIAAQFCDVQLFHFFKRLTKGRHLWLRNNFSTLTSQMVDSFMVISVTFGAAYFA
                     GSITLAAMLVLMGSNYLFKMTVALADTLPFYYLTGRLKRYLQIDNVHVLE"
     gene            complement(3672..4607)
                     /locus_tag="AC16UZ_12775"
     CDS             complement(3672..4607)
                     /locus_tag="AC16UZ_12775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6247602.1"
                     /translation="MQMRLGITPWNLADRTASGLTEQARHAERLGYDSFWLPENHFNP
                     GAIPDPLMLLAAVAAGTQKIRLATTSYLLPLRNPLLAAEQVAVLDQLSGGRVTLGIGR
                     GYAPVMLRAFDVRPSEKRELFERSLALMRQAWRGEPVVLDDGETTVTLDPLPVQRPHP
                     PLWVAAFGPKALAQAGRLGLPYLASPVETLDTLEANYRRHGEAAAEAGHGPRDTIPVM
                     RTVFVSSNPRETRELLRRMNAEAEKSGRLAPGAEAHEWTIVGDDQFVRDRIVEYRERL
                     GVNHLIVTRLRLGGVDEGAVQASVGRVADIVGGLP"
     gene            complement(4625..>5863)
                     /locus_tag="AC16UZ_12780"
     CDS             complement(4625..>5863)
                     /locus_tag="AC16UZ_12780"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6247603.1"
                     /translation="PSGRDESGGDTEGGGGPLAGLKVLDLFWVVAGPGATRMLADYGA
                     TVVHVESTRRQDMVRNVPPYIDGVPDPERAACHHSTNTNKLNLTLDISREEGRAVLAD
                     LIRWADVFTESFAPGAVARMGFDYATVRTLNPNIIMISSCLMGQTGEWSGYAGFGNNG
                     SAVSGFHALTGHPGQPPTGVFGPYTDFTSVRFNALAILGAVMHGRRTGNGQYIDMSQG
                     EAALHFLAPECLRYLRDGRVRGAEGNRDEHFAPQGVYPTAGFDRWLALSVRSDAEWRA
                     LCALAGFDDLSNMSVEERAAAHDELDRRLETWMETRPGEELEAELQSAGIAAHRTLDT
                     HELGDDPQLVHRGHFIPVAHREFSNAVVESSRLTLSASRAARPGTAPWFGIDNERVLS
                     EILGYDGDRVRELASADVLS"
BASE COUNT         1029 a         1977 c         1849 g         1008 t
ORIGIN      
        1 acgaccggtc tggcccccga gccgcgagcc gcgacgcttg agctcgtccc acaaaccgac
       61 gatctcgtct tccggccagt cggccaggta gcgcccgaag ccaccggctt cacgccgcac
      121 gtccagaatg aaggccgcgt tggcaggaac cgaacggatc ttcgctgcgt gacgcacgat
      181 ccgctcgtcc gccgagagct gctcgatgtc ttcatcggac agcatcgcac agccggccac
      241 atcgaacccg cggaatgcaa cctcgaaccc cggccacttg ttctcgatga ttttccacac
      301 gaaacccgag cggaagacac acctggtcat ctcggcgaga tagtcccggt ccggcaaggc
      361 ggccagatcg ctgcctgacc tggctgcggg cagcagcgcg tctagggccg ccctgccccc
      421 cttgcgctca accgccctgt gcaagatgct atcgaacgat tccatatccc gcggaaccca
      481 aagagccacg acgaacacca tggtggatgt cctcgacaaa acccgtcaag acctagaccc
      541 gaagctcaag gagatcgtcg agaaggacga gcggaatttc gtctactgcg ggatctgctc
      601 gcacgtgctg acccgggagg tcgagcgcat cgaggtgtcg ggcagtcacg atcatcattg
      661 cacgaacccg ttcggtcttc gctttcatat cggctgcttc gccgaggccc tgggctgcga
      721 catctcggga gagcccatgg cggcagacac ctggtttccg ggcttccgct ggcgccacgc
      781 atcctgcgcg gagtgtcacc agcatctggg ctggtatttc gaagcaggcg aaagatactt
      841 ctacggattg atactggacc gcattcagca tgactgacct cgctgccggc aactaccgca
      901 cgctgacctt cgactgctac gggacgctca tcgattggga gaacggcatc ctcggctacc
      961 tgcagcccct gctcgagagc tacgacgtgc acgtgctcga cgagtgggtg ctggaattct
     1021 ttgcggagaa cgaacccgcg ctgcaggccg agggcggctc gtatcgatcg gtgctcgcgc
     1081 gtctgctgga gcggctcggt acgcggtgcg cgttcacgcc caacgatgac gccctgaagg
     1141 gctttgcaga cagcatcgag tactggcagc cgttcgcaga caccaacgcc gcgttgcgcg
     1201 ccctgtccga gcacttcgac ctggcagtgg tctcgaatgt cgacaacgag ctgttctccc
     1261 tctccgccag gcagctgggc gtggagttcg cgcaggtgat cactgccgat gacgtcgggg
     1321 cctacaaacc cgcccgcgcg atgttcgacg aggcgctcag aaaggtatcg gggcccgtgc
     1381 ttcacgtcgc ccagagcctc taccacgaca tcgcgcccgc caaagcgctg ggtctggaca
     1441 ccgtgtggat caaccggccg agcgcggccg gcagcggggc cgcgcgtttc gtcgaggtgg
     1501 aaccgacctg gacgttcggc gatctcagcg agctcgtcga ggccttcgac ctcgactgag
     1561 ccgatgcgga attcacccgt tcgcacgttt tcgctgtgat aggatcgccc gatgtcgcga
     1621 ccgtctctca ccagcattca gatcagcaag gaataccttc atttcaactc cgctcacttc
     1681 accctgttct ccgatcgcga acgggaggac ctgcacggcc acacctttta cgtggcgggt
     1741 gctttcgatg ccgaggtggg tccggacggg ctggcgttcg attacaacct ggtcaagacc
     1801 aagctgaaac agctgtgcga cgagctggac gagaaaaccc ttctgcccga acgttctccc
     1861 cacctgcgcc tgagcgagga aaacggctat ctcgtcgccg agttcgccgg cgagcgcatg
     1921 ccctttctgc cacgggacgt cctcaccctt cccgtgcgca acgtcacggt agaggaactg
     1981 gcgccgtttc tgctggagcg tctcggctcg gatcccgaga tccgcacgct cgagctgcag
     2041 aggattgagc tgcgcgtctc ttcaggaccc ggccagtggg ctgcggcgac ctggaccgcc
     2101 gggcaagaga tacgggaggt gtaagccatg aagatactgc tcattaccgg agccagcgcg
     2161 gggatcgggc tgcacactgc ccaacgcttc gttgccgaag gattcgccgt ggtgaacctc
     2221 tcgcggcgcc gctgtcccat cgacgcggtc agccagatca actgcgacct gtcggttccc
     2281 gggttcatcg acaacatcag caatcagctc agcggaatgc tgcaggaggc cgaccgggtg
     2341 gtgctcgtgc acaacgccgc ccggatgggc aacgacacgg cggtggagac cccgagcgtg
     2401 gccctgcgcg aggcgctgga ggtgaacgtg gtcgcaccga acacgctcaa ctacttcacc
     2461 atcccgtaca tgaaaccggg ctcggccatc atctacgtgg gctccaccct ggcggagaag
     2521 gcggtacccg gcacgtatac ctatgtggtg agcaagcacg ccctgatcgg aatgatgcgc
     2581 tcgacctgcc aggatctggc ggggcagagc atccataccg cgtgcgtttg cccgggcttc
     2641 acggataccg agatgctgcg tcagcacgtt ccggccgaag ccatggcgag catccgcgcc
     2701 atgtcggcct acggacggct gatcgacccg gacgagatcg ccgaaaccat cttctgggcc
     2761 gccagcaacc cggttatcaa cggggccgtt ctgcacgcca atctgggtca gcgggaaagc
     2821 tgatgcaggg aggcgtaaga acagcggggg gcaacgagga aacgacggga aaccgcctca
     2881 gcgagcagga cctgaacgag cgacgcgagc gggtgttcat cgttctggcc ggctttttcc
     2941 tctgcgccat gacgctgcta aacgtcgtcg gcatcacccg tttcatacag ctgggcccca
     3001 tggccctggc ggtgggcgtg ctgccctacc cgctcacctt cctgtgcacg gatctcatca
     3061 gcgagctgta cggccgcaaa cgcgccaact tcctggtctg ggtgggcttc accctgaact
     3121 tcttcattct cggcatgctc tggctcggca gctcgatacc ggccgtagat cccgccacgc
     3181 agccgccctg gcaggtcatc gagctggccg aggagattgc gctgcccaac ggcgacgtcc
     3241 tcagcggcag cgtggagctg tttcagctgc tctatgcctg cacctcgggc gccgtgttcg
     3301 cttcgatgat ggcctacatc gccgcgcagt tctgcgacgt gcagcttttt cacttcttca
     3361 agcggctgac caagggtcga cacctgtggc ttcgcaacaa cttttccacg ctgacgagcc
     3421 agatggtgga ctccttcatg gtcatcagcg tcactttcgg cgcagcctac ttcgccggca
     3481 gcatcacgct ggcggccatg ctcgttctga tgggcagcaa ctacctcttc aagatgacgg
     3541 tggcgctggc agacactctg cccttctact acctcaccgg ccgcctcaag cgctacctgc
     3601 agatcgacaa cgttcacgtc ctggagtgac cggcccgatc ggatcgctcc ggcgtgatcc
     3661 ggtggaggca gtcagggcag accgccgacg atgtccgcca cccgcccgac ggaagcctgc
     3721 acggcacctt cgtccacccc gccgagcctg agtcgcgtca cgatcaggtg attgaccccc
     3781 agccgctcgc ggtactcgac gatccggtcc ctgacgaatt ggtcgtctcc gacgatggtc
     3841 cactcgtgag cttccgcgcc gggcgccagg cgaccagatt tttccgcttc cgcattcatc
     3901 cggcggagca gctcccgtgt ctctctcggg ttggacgaaa cgaataccgt gcgcatcacc
     3961 gggatcgtat cgcgaggccc gtggccggcc tccgctgccg cctcgccgtg acgccgataa
     4021 ttggcttcga gcgtatcgag ggtctccacg ggcgacgcga ggtaaggcag cccgagacgg
     4081 cccgcctggg cgagtgcctt cggcccgaag gcggcgaccc acagcggcgg gtgcgggcgc
     4141 tgaaccggca gcggatccag agtgacggtg gtctcgccgt catccagcac gaccggctca
     4201 ccgcgccacg cctgccgcat gagggctaag gagcgctcga agagctcgcg cttctcgctg
     4261 ggccggacgt cgaacgctcg aagcatgacc ggcgcgtacc cccgaccgat cccgagcgtg
     4321 acccgaccgc cggagagctg atcgagcacg gcaacctgct cggcagccag cagcggattt
     4381 cgcagcggca gcaggtacga cgtggtggca agacggatct tctgcgtgcc ggccgccacc
     4441 gcggccagca gcatgagcgg atccggtatg gcgccggggt tgaagtggtt ttccggaagc
     4501 cagaacgaat cgtacccgag ccgctcggca tgccgggcct gctcggtcag cccgctggcg
     4561 gtgcgatcag cgagattcca gggcgttatg ccaagccgca tctgcatgcc acctcaccac
     4621 gccgtcacga cagcacgtcc gcagatgcca gctcgcggac ccggtcgccg tcgtagccga
     4681 gaatctcgct tagcacgcgc tcgttgtcga tgccgaacca cggcgccgtg ccgggtcggg
     4741 cggcgcggct tgccgacagg gtcagccgtg agctttccac cacggcgttc gagaactccc
     4801 ggtgcgcaac gggaatgaag tgaccccggt gaaccagctg gggatcgtcg ccgagctcgt
     4861 gggtatccag ggtccggtgg gcggcgatgc cggcagattg cagctccgct tccagctcct
     4921 ccccggggcg ggtctccatc cacgtttcca ggcgccggtc gagctcgtcg tgagcggccg
     4981 cccgttcctc gaccgacatg ttcgagaggt cgtcgaatcc cgccagggca cacaacgcgc
     5041 gccactcggc gtcgctgcgc accgaaagcg ccagccagcg atcgaacccg gcggtcgggt
     5101 agacgccctg gggggcgaag tgctcatcgc ggttgccttc ggcacccctg acccgaccat
     5161 cgcgcaggta acgcaggcac tcgggcgcga gaaagtgcag cgccgcctcc ccctgggaca
     5221 tgtcgatgta ctggccgttg ccggtccgac ggccgtgcat cacggcgccg agaatggcca
     5281 gggcgttgaa gcgcactgag gtgaaatcgg tataggggcc gaagacgccc gtgggcggct
     5341 ggcccggatg gccggtgagc gcgtgaaagc ccgacactgc cgagccgttg ttgccgaacc
     5401 ccgcgtagcc gctccactcc cccgtctggc ccatcaggca gctcgagatc atgatgatgt
     5461 tcgggttgag cgtcctcacc gtggcgtagt cgaagcccat ccgggccacc gcgccggggg
     5521 cgaaggattc ggtgaagacg tcggcccagc ggatcaggtc ggcaagaacc gcacgaccct
     5581 cttcccgact gatgtccagc gtcaggttga gcttgttggt gttggtgctg tgatggcagg
     5641 cggcgcgctc cgggtccggg accccgtcga tgtagggcgg cacgttcctg accatgtcct
     5701 gccgccgagt ggactccacg tgcaccacgg tggccccgta gtcggcgagc atgcgcgtgg
     5761 cgccggggcc ggccaccacc cagaaaaggt cgaggacctt caatccggcc agcggcccgc
     5821 cgcccccttc ggtgtccccg cccgactcat cccggccgga cgg
//
LOCUS       DBNHOX010000583         9242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1140587, whole genome shotgun sequence.
ACCESSION   DBNHOX010000583 DBNHOX010000000
VERSION     DBNHOX010000583.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1140587"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="AC16UZ_12785"
     CDS             complement(<1..406)
                     /locus_tag="AC16UZ_12785"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0042178 - xenobiotic catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HIR6247604.1"
                     /translation="MYTLYEMGDSGNCYKIRLLMHQLDVPFERVPTDILQGRSRTESF
                     LALNPNGKVPLLVLPDGRPLAESNAILSYLARGTAYLPDEAYEQALVLQWLFFEQYSH
                     EPYIATNRFWIHILKDPVGHAQEIAARHPKGEA"
     gene            518..1567
                     /gene="nadA"
                     /locus_tag="AC16UZ_12790"
     CDS             518..1567
                     /gene="nadA"
                     /locus_tag="AC16UZ_12790"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR6247605.1"
                     /translation="MSEATLPDYTLSADRVPVYEPEVLGPERVADLKSEIADLLAARG
                     AVLVAHYYVDGHVQDLAEETGGFVSDSLEMARFGHDHAAETLVVAGVRFMGETAKILS
                     PEKRVIMPTLEAECSLDIGCPPDDFEAFCKANPDRTVVVYANTSARVKAMSDWVVTSS
                     VALPIVEMLDARGEKILWAPDKHLGHYIQTQTGADMLLWSGACVVHEEFKSKALFDLK
                     AVYPEAGVLVHPESPAAVISIADVVGSTSQIIKAAQELPHDTFIVATDRGIFHKLRQR
                     NPDKRFIEAPTAGEGATCRSCAHCPWMAMNELSTLAAMLRSEGAMTANEIHVDPEIGR
                     RAMIPLKRMLDFKVP"
     gene            complement(1564..2994)
                     /locus_tag="AC16UZ_12795"
     CDS             complement(1564..2994)
                     /locus_tag="AC16UZ_12795"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR6247606.1"
                     /translation="MIRLGIGAALCLLTAAASTDVSDDLPVLGDASSSMISPELERQI
                     GRDFLKQVHAGLPTISDPVLKHFVTRQLTDLLQYSQVRENLLGVVLIDSDQINAFAAP
                     GGVVGVNLGLLLHAQDVHEYSSVMAHELAHLSQRHFARGVEEARSKTLPTIASLLAAI
                     MIGAVGGGEAGMAAISASQAASQSSQLRYSRGREQEADRIGLNTIVRAGMDPDGMARM
                     FERMQRNYRFSRTPPEFLLTHPLSETRISDAKSQAREYPQRDYADSPDYAMMRVRAQI
                     YYAPNAAGAVAQFEKAVRDDPESVPARYGLALAQSRAGLHAEAIAGGDALFTADPQKI
                     LYIASYAELLIEAEKLDQATRLLSHHLALNPDNATLAAMYSEALIRDQRYREAEAVLE
                     RQSVVNKDDVDVWYNLAEVSGKAGNIIGVHRARAEYFALHGAYQRAIQHLEYARRLVR
                     EDNQQLHAMLDQRINDMRMALRMRRS"
     gene            3171..3404
                     /locus_tag="AC16UZ_12800"
     CDS             3171..3404
                     /locus_tag="AC16UZ_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR6247607.1"
                     /translation="MTEPVVVDAKGLNCPMPLLKAKKALNELSPGELVKVLATDPGSV
                     RDFEVFSRQSGHALLESLREDDTYIYLLRKKPG"
     gene            3404..4507
                     /locus_tag="AC16UZ_12805"
     CDS             3404..4507
                     /locus_tag="AC16UZ_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6247608.1"
                     /translation="MRFLEVIGGWINRHFSNEEAIYLVVLIVIISTVVFIFGSMLAPV
                     LIGLIVAFLLQGLVKRLVDAGLGGGLAVYLTFVIFLGALIALVLFVVPIVWQQLRALV
                     DALPNGIVQVQALIRDLANTSPGGLVTPEQVDKWLGSLTGELGNLSRRLLNTLLTQVP
                     SVVALMIYTVIVPFSVFFFLKDRDELMRWFLSLLPEERPLLDRVGAEMNLQLANYVRG
                     KFVEILIVGVTTYVTFVLFGLNYAALLAVLVGLSVLIPFVGAAVVTIPVALVAVVQFG
                     WSLPLAGVLGAYLIIQFLDGNFLVPLLISSAVNLHPITIISAVLAFGGLWGIWGVLFA
                     IPLATLVKSIYNAWPRQEELAEASGDGLVEVDG"
     gene            4589..5464
                     /gene="dapA"
                     /locus_tag="AC16UZ_12810"
     CDS             4589..5464
                     /gene="dapA"
                     /locus_tag="AC16UZ_12810"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR6247609.1"
                     /translation="MFTGSIVALITPMTAGMEVDWTALERLIGWHLEEGTDGIVPVGT
                     TGESATLNIEEHLEVIRRTVSVVSGRVPVIAGTGANATAEAIHLTREAQKIGVDASLS
                     VTPYYNRPTQEGLYRHYAAIAEAVDLPIVLYNVPPRTGRDMTADTVARLAEIGNIVGI
                     KEACGDPERVAEIRGLVAEDFFVLSGEDGQTLRMFELGAVGCISVTANVLPRRMAEFC
                     RAWREGDVDLARAIDSELQPIHEILFVEPSPQPTKWALYEMGMVDRGIRLPMLELSEE
                     NRPELIRRLKAVGAL"
     gene            5482..6594
                     /gene="bamC"
                     /locus_tag="AC16UZ_12815"
     CDS             5482..6594
                     /gene="bamC"
                     /locus_tag="AC16UZ_12815"
                     /inference="COORDINATES: protein motif:HMM:NF018509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HIR6247610.1"
                     /translation="MRVARALLVVPLVAGCSWLNDDEGIFVNKSDDYLDAREGPGLYV
                     PEDLATGRVSDPFPIPPSPEQMNPEFYPQRPPQPSAIYASDTRDEVRIQRLGDRRWLA
                     LPESPTTVWPKLKQFLAENGVPIALEMGPEGRIDTDWLLISEDTYRDVVRLVIRDGRD
                     IAELTTGRDRIRFRLEQGMRERTCEVHIRHENDSLGLPAADSLVDIEGVASDIPDIEN
                     DMLNEFGAYVAAKVSEQTVSMVAQEISAGAKSVLTRNEQGDPTLILYLDYERAWAALG
                     QAIARAEMDVTDQNRDEGTYYVNITEAFLAGEEKKGFFGRMFSFGGGEDAKELQIQME
                     RSGEEIYSVSVLDENAAPVDRELGQQLLIMIREFAS"
     gene            6591..7382
                     /locus_tag="AC16UZ_12820"
     CDS             6591..7382
                     /locus_tag="AC16UZ_12820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6247611.1"
                     /translation="MTAFASLGSGSRGNGTLVDLGGELILVDCGFTLKQAENRFRRLD
                     VRPGDLSAILVTHEHADHAGGVAALSHKYAVPVFASHGTFKATRNELVGRDIDSHASF
                     DVGAVTVMPVIVPHDAREPTQFVFQHGAVRIGVISDLGQITPFVIEQYQGCDGLMMES
                     NHDREMLLRGRYPESVKRRIAGNLGHLSNEQAASFLEQVGHADLRVAVGHVSEENNHP
                     ELLEETFARFSGQVAELTFATQEDGLGWIRLPEGGVSAAAALGAG"
     gene            7441..7531
                     /locus_tag="AC16UZ_12825"
     tRNA            7441..7531
                     /locus_tag="AC16UZ_12825"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7476..7478,aa:Ser,seq:cga)
     gene            complement(7622..8452)
                     /locus_tag="AC16UZ_12830"
     CDS             complement(7622..8452)
                     /locus_tag="AC16UZ_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6247612.1"
                     /translation="MNHVEVYGADHSPWVQAVLLGLHDAGIPHSLTTLPPLETFRKSG
                     VMMPAARIDEGDWQLESADILRQIGFSAVSEVEMKQVRSAWRGVLHRADSASLFWGGF
                     SLIGDRDTSPPRRLIKNFLRSFVTLYFYVLIRTAVLTGPPRDPENFGDQFLPFEAMLE
                     ESGKPYLAGDGPDTLDFLLFGIIQCHCSIYVPPVAALQSDPRLQRLRGWIARMHQRFS
                     NYDHLYSGAYFAPHSRRPAWASPLERIVFWLGAAFMIAAFPITIPSIAILTIRNRNFG
                     "
     gene            8566..9072
                     /locus_tag="AC16UZ_12835"
     CDS             8566..9072
                     /locus_tag="AC16UZ_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247613.1"
                     /translation="MLKIPAALAVVVVVGIGVLAAIARSSDGPLAIFPGGAFSSGERY
                     TGSEPDWEALVDVDTVEFQSLDPVRSRTTWIAVHHGRIFIPSGYMTTWWGRIWKQWPI
                     EAERDGRVILRADGKLYDRELVRVSSGPELEPVLAELGRKYGGGQAFPMDAVTNGYLW
                     IFELAPPR"
BASE COUNT         1631 a         2823 c         3004 g         1784 t
ORIGIN      
        1 ccgcctcgcc cttgggatgt ctggccgcaa tctcctgcgc gtggccgacg gggtccttga
       61 gtatgtgaat ccagaatcgg ttggtggcga tatagggctc atggctgtac tgctcgaaga
      121 acagccattg cagcaccagc gcctgctcgt aagcctcgtc cgggaggtag gccgtacctc
      181 gcgcaaggta gctcagaatg gcgtttgact ccgccagcgg gcgcccgtca ggtagaacga
      241 gcagcgggac cttgccattg ggattcagcg ccaggaacga ttccgtgcgc gaccgcccct
      301 ggaggatgtc ggtgggtact cgctcgaaag gaacatccag ctggtgcatc aatagccgga
      361 tcttataaca gtttcccgag tctcccatct cgtaaagcgt gtacatcgaa cctccttggt
      421 tttcgtcgat tcgccccaat aaaaagatgg tcatataaac aggcgacggt tgcacgggaa
      481 ctgtgatttt ccctgcgctt ccaatattct cgatgtcatg agcgaagcta cgctgccaga
      541 ctacaccctc tccgcggacc gggtacccgt ctacgagccg gaggtgctcg ggccggaacg
      601 ggttgcagat ctgaaatccg agattgccga tctgctcgcc gcccgcggcg ccgtgctggt
      661 ggcccattac tacgtggacg gtcacgttca ggacctcgca gaggaaacgg gcgggttcgt
      721 ctccgactcg ctggaaatgg cccgattcgg gcacgaccac gccgctgaga cgctggtggt
      781 cgcgggcgtg cgtttcatgg gcgagaccgc caagattctc tcgccagaga agcgggtgat
      841 catgccgacg ctcgaggccg agtgctctct ggacatcggc tgcccgccgg acgacttcga
      901 ggccttctgc aaagccaatc ctgaccggac ggtggtcgtc tacgccaata cctcggcccg
      961 ggtcaaagcg atgtccgact gggtggtgac cagcagcgtg gccctgccca tcgtggagat
     1021 gctcgacgcc cgaggtgaaa agatactctg ggcccccgac aagcatctgg gacattacat
     1081 tcagactcag accggcgccg acatgctgct gtggagcggc gcctgtgtgg tccacgaaga
     1141 gttcaagtcg aaggcgctct tcgacctaaa ggccgtttat ccggaggcgg gcgtcctcgt
     1201 gcaccccgag tcaccggccg cggtgatttc catagccgac gtggtgggct cgaccagcca
     1261 gatcatcaag gcggcccagg agctgcccca cgacaccttc atcgtggcca ccgaccgcgg
     1321 catcttccac aagcttcgcc agcgcaaccc ggacaagcga ttcatagaag cgcccacggc
     1381 cggcgagggc gcaacctgcc gcagctgtgc ccactgccct tggatggcga tgaatgagtt
     1441 atccaccctg gcggccatgc tgcgcagcga aggggcgatg acggccaacg agattcatgt
     1501 ggaccctgag atcggtcgtc gggcgatgat cccgctcaag aggatgctcg acttcaaggt
     1561 gccctaggac cgccgcatcc gcagcgccat ccgcatgtcg ttgatgcgct gatccagcat
     1621 ggcgtgaagc tgctggttgt cttcccgcac caggcgccgc gcgtattcca gatgctggat
     1681 ggcccgctgg taggcgccgt gcagcgcgaa gtactcggcg cgagcccggt gcacgccgat
     1741 gatgttgccc gctttgcctg atacctcggc gaggttgtac cacacgtcca cgtcgtcctt
     1801 gttgacgacg ctttgccgct ccagcaccgc ctcagcctcc cggtaccgct ggtccctgat
     1861 gagcgcctcg ctgtacatgg ccgccagggt ggcgttgtcc gggttgaggg ccaggtgatg
     1921 cgacagcaga cgggtcgcct gatcgagttt ctccgcctcg atgagcagct ctgcgtatga
     1981 cgcgatgtag agaattttct gagggtcggc ggtgaacaga gcatcaccgc cggcgatggc
     2041 ctcggcgtgc agacccgctc tgctctgcgc cagggccagc ccatagcgcg cggggaccga
     2101 ctcgggatcg tcccgcacag ccttctcgaa ctgggccacg gccccggcgg cgttgggcgc
     2161 gtagtagatc tgggcgcgga cccgcatcat ggcgtagtcg ggtgagtcgg cgtagtcgcg
     2221 ttgggggtac tcgcgggcct ggcttttcgc gtccgagatg cgggtctcgc tcaacgggtg
     2281 ggtcagcagg aactccggcg gcgtgcggga aaagcggtag ttgcgctgca tcctctcaaa
     2341 cattcgcgcc atgccgtccg gatccatgcc ggcccggacg atggtgttga ggccgatccg
     2401 gtcggcttcc tgctcgcggc cccggctgta gcgcagctgg ctggactggc tggccgcctg
     2461 ggaagcagat atggccgcca tgcccgcttc gcccccgccc acggcgccga tcatgatggc
     2521 ggccagcagg gaggcgatgg tgggcagggt tttgctgcgg gcctcctcga cgcccctggc
     2581 gaagtggcgc tggctgaggt gagcaagctc gtgtgccatg accgaggagt attcgtgcac
     2641 gtcctgggca tgcagcagaa gtcccaggtt gacgcccacc acgccgccgg gggcggcaaa
     2701 ggcgttgatc tgatcgctgt cgatgagcac cacgcccagc aagttttccc tgacctggga
     2761 gtattgaagc aggtcggtca gctggcgcgt gacgaagtgt ttgagaacgg ggtcgctgat
     2821 ggtcggcagg cccgcgtgca cctgcttgag gaaatccctg ccgatctggc gttcgagttc
     2881 cggggagatc atgctcgagg aggcgtcgcc gagcacgggc aggtcgtcgc tgacgtccgt
     2941 ggaggctgcg gccgtgagca ggcacaacgc agcgccgatg ccgaggcgaa tcacggcccc
     3001 ggcgccactc agacgtcgct gcttcgctcg ggagtcttgc attctggaga tcatactgga
     3061 tggcggtgtg ccgcagaacc tgctctggtt ttagccacct gcgggcggga aaagttcaaa
     3121 agtcttatga taggcatcct gctgccgctc atccgtgcgc tgaaccgaac atgaccgaac
     3181 ccgtagtggt cgatgccaag ggcctgaact gcccgatgcc gctccttaaa gccaagaaag
     3241 ctctcaacga gctctccccg ggcgagcttg tgaaggtcct cgccaccgat cccggatcgg
     3301 ttcgcgactt cgaggtgttt tcgcggcaga gcggccacgc tctgctggaa tcgcttcgcg
     3361 aggacgatac ctacatctac ctgctgcgaa agaagccggg ctgatgcgct ttctagaggt
     3421 cataggcggc tggatcaacc ggcacttctc caatgaggaa gccatctacc tggtggtcct
     3481 catcgtcatc atctctaccg tggtgttcat cttcgggtcg atgctggccc cggtgctgat
     3541 cgggctcatc gtcgcctttc tcctccaggg tttggtgaaa cgtctggtgg acgcggggct
     3601 cggcggcggg ttagccgttt acctgacgtt cgtcatcttc cttggcgccc tgatcgccct
     3661 cgttctcttc gtggtgccca tcgtctggca gcagcttcgt gccctggtcg acgcgcttcc
     3721 gaacgggatc gtgcaggtgc aggccctcat ccgtgatctg gccaacacct cgccgggtgg
     3781 gctggtgacg cctgagcagg tagacaagtg gctgggctcg ctgacgggag agttgggcaa
     3841 cctgagccgc aggctgctaa acacgctcct aacccaggtc ccgtccgtgg tggcgctgat
     3901 gatctacacg gtgatcgtgc ctttctcggt gttcttcttc ctgaaggatc gtgacgagct
     3961 catgcgctgg ttcctctctc tgctccccga ggagcgcccg ctgctggacc gggtgggcgc
     4021 ggagatgaac cttcagctgg ccaactacgt tcgcggcaag tttgtcgaga tcctcatcgt
     4081 tggcgtgacc acctacgtga ccttcgtgct gttcggtctc aattacgccg cgctcctagc
     4141 ggtgctcgtg gggctctcgg tgctgattcc gtttgtcggg gcggccgtgg tgacgatccc
     4201 cgtcgcgctg gtggccgtcg tgcagttcgg atggtccctg cccctggcgg gcgtgctggg
     4261 cgcgtacctg atcattcagt tcctggacgg caattttctg gtcccgttgc tgatttccag
     4321 cgccgtcaac ctgcacccca tcaccatcat ctctgccgtt ctcgctttcg gcggcctgtg
     4381 ggggatatgg ggggttcttt ttgccattcc tctggcgacg ctggtgaagt ccatctacaa
     4441 cgcctggccg cgccaggagg agttggcgga agccagcggg gacggcctgg tcgaagtcga
     4501 tggttgagcc ccgttgcgcc gctcaagtac catggcgcgg ttctgctccc cgcgtcccta
     4561 gggccgggtc gcccagctag agagcgatat gttcacaggt agcatcgttg ccctgatcac
     4621 gcccatgacc gcggggatgg aggtcgactg gaccgccctg gagcgtctga tcggctggca
     4681 tctggaggaa ggcacggacg gtatcgtccc cgtgggaacc acgggcgagt ctgccacgct
     4741 caatatcgag gagcacctcg aggtcatccg gcggacggtg tcggtggtgt ccggacgggt
     4801 accggtgatc gcgggaaccg gcgccaacgc gacggcagag gccattcacc tgacacggga
     4861 agcacagaag atcggcgtgg acgcgagcct gtcggtcacg ccctactaca accgcccgac
     4921 ccaggaaggt ctttatcgtc actacgcagc gattgccgaa gcggtggatc ttcccatcgt
     4981 gctctataac gttccgccac gcaccggtcg cgacatgacg gccgatacgg tggcgcgtct
     5041 tgccgaaatc ggcaacatcg taggcatcaa ggaagcgtgc ggcgacccgg agcgggttgc
     5101 cgagatccgc ggtctcgtcg ctgaggactt cttcgttctg tctggagagg atgggcagac
     5161 gctgaggatg ttcgaactcg gcgcggtggg ttgcatctcg gtgaccgcca acgtgctgcc
     5221 gcgcagaatg gccgagttct gccgtgcctg gcgtgagggg gacgtggacc ttgcgcgggc
     5281 catcgattcg gagttgcagc cgattcacga gatcctgttc gtggagccga gtcctcaacc
     5341 gacgaagtgg gcgctctacg agatgggtat ggtggatcgc ggcatacgtc tgcctatgct
     5401 ggaactttca gaggaaaacc gtccggagct gatccggcgc ctgaaggcgg ttggggctct
     5461 gtgatgcgcg cgcactgcct gatgagagtc gcgagagcgc tgctggtcgt gccgctcgtg
     5521 gcgggatgct cgtggctcaa cgacgacgag ggcatcttcg tcaacaagag cgatgactac
     5581 cttgatgccc gggagggccc cggactttat gttcccgagg atctggccac cgggcgggtg
     5641 tccgacccct ttcccatccc gccgtctccc gagcagatga atccggagtt ctacccgcag
     5701 cgcccgccgc agcccagcgc catttacgcc agcgacaccc gtgacgaagt ccgcattcag
     5761 cggttgggag acaggcgctg gctggcgctg cccgagtcgc ccaccacggt atggccgaag
     5821 ctcaagcagt tcctggcgga gaacggcgtg cccatcgcgc tggagatggg tccggaaggg
     5881 cgcatcgaca cggactggct gctcatcagc gaggacactt atcgtgacgt ggttcgcctt
     5941 gtcatccgcg acggccggga cattgccgag ctgaccacgg gccgcgaccg aatccggttc
     6001 cggctcgagc aagggatgcg tgagcgaact tgtgaggttc acatccgtca tgagaacgac
     6061 agcctggggt tgcctgctgc agactctctc gtggacatcg agggcgtcgc ctcagacatc
     6121 ccggacatcg aaaacgacat gctcaacgag ttcggcgctt acgtcgctgc caaagtatcc
     6181 gagcagaccg tttccatggt ggcacaggag atctcagcgg gcgcgaagtc ggtgctcacc
     6241 cgtaacgagc agggagatcc gaccctcatc ctgtacctcg actacgagcg ggcttgggcc
     6301 gccctgggtc aggccatcgc ccgggccgag atggacgtca ccgaccagaa tcgcgacgag
     6361 ggcacctact acgtgaacat caccgaagcg tttctggccg gagaagagaa aaaagggttc
     6421 ttcggccgga tgttctcgtt cggcggtggc gaagacgcca aagaacttca gattcagatg
     6481 gagcgttccg gggaggagat ctactcggtt tccgtcctcg atgagaacgc ggctcccgtc
     6541 gaccgggagc tggggcaaca gctgctgatc atgatccgcg agtttgccag ttgacggctt
     6601 ttgcctcgct gggcagcggc agccggggca acggtacgct cgtcgatctg ggtggagagc
     6661 tgatcctcgt ggactgcgga ttcaccctga agcaggccga gaaccgcttt cgccgtctcg
     6721 atgtccggcc cggcgatctc tcggcgatcc tggtcaccca cgagcacgct gaccacgctg
     6781 gtggtgtcgc ggcgctcagc cacaaatacg cggttcccgt gtttgcttcc cacggaacct
     6841 tcaaggccac ccgcaatgag ctcgtgggca gggacatcga ttcccatgcc agcttcgacg
     6901 tcggcgccgt gaccgtgatg ccggtgatcg taccccacga cgcccgggag ccgacgcagt
     6961 ttgtcttcca gcacggtgcc gtccgcatcg gcgtcatctc cgacctcggc cagattaccc
     7021 ctttcgtgat cgagcagtat cagggctgcg acggcctgat gatggaaagc aaccacgacc
     7081 gcgaaatgct cctgcgcggc aggtatccgg aaagcgtgaa gcgccgcatc gccggcaatc
     7141 tcggccatct atccaacgag caggcagcgt cgttcctgga gcaggtgggt catgcggacc
     7201 tgcgcgttgc cgtcggccac gtcagcgagg aaaacaacca ccccgagctg ctggaagaaa
     7261 ccttcgcccg gttcagcggc caggttgccg agctgacatt cgccactcag gaggatgggc
     7321 ttggctggat ccgcctgccc gagggcggcg tcagcgcggc cgccgccttg ggcgccggct
     7381 aatcgctgga aaacgtccct cggtttgctc agaatgtcgc ccttgctgat ctgacctcac
     7441 ggagaggtgc cggagctggc cgaacgggct tgactcgaaa tcaagtgacc tcgcaagggg
     7501 tccgtgggtt cgaatcccac cctctccgcc agtcacccac tcctatcgct caggcaaacg
     7561 ccagcgtggt cgcaatcgca ccagtgatat ttggcagcag ccggcgcctg tgacgatcca
     7621 atcacccaaa atttcggttg cgaatcgtca gaatggcgat cgacggtatc gtgatgggga
     7681 acgcggcgat catgaatgcc gcgcccagcc agaacacgat gcgttccagt ggagacgccc
     7741 aggcgggccg gcgcgagtgc ggagcgaagt aggcgcccga atagagatgg tcgtagttcg
     7801 agaagcgctg atgcatgcgg gcgatccagc cgcgcagcct ctgcaggcgc gggtctgatt
     7861 gcagcgccgc cacgggcggt acgtatatgc tgcagtggca ctgaatgatg ccgaacagca
     7921 ggaagtccag cgtgtcgggc ccgtcgccgg cgaggtatgg cttgcccgat tcctcgagca
     7981 tcgcctcgaa tggcagaaac tgatcgccaa aattttcagg atccctgggc ggacccgtta
     8041 gaaccgcggt tcgaatcagc acgtagaaat agagagtcac gaagctgcgc agaaagtttt
     8101 tgatcaggcg tcggggaggt gacgtgtccc tgtctccgat caggctgaag ccgccccaga
     8161 acagagatgc gctgtcggct cgatgcagaa cgccccgcca ggcgctgcgg acctgcttca
     8221 tctcaacctc agagacggcc gagaacccga tctgtcgcag tatgtccgcg gactcgagct
     8281 gccagtctcc ttcgtcgata cgcgctgcgg gcatcatgac ccccgacttc cggaacgtct
     8341 ccaggggcgg cagcgtggtc aaagagtgcg ggatccccgc gtcgtgcaat cccagcagaa
     8401 cggcctggac ccaaggagag tgatcggctc cgtagacttc gacgtgattc atcgcgatca
     8461 gctctggctg tgaatcctga tttgtggatg atgacagaaa gctaggccgt ggccgatggt
     8521 gctgttaccg aacgagtagc caggggggga taacctatca agactttgct caaaattccg
     8581 gcagccctgg ccgtcgtcgt ggtcgtcggc atcggtgtat tggctgccat cgcccgctcg
     8641 tcggacggtc ccctggcgat cttcccgggc ggggcgtttt cgagtggtga gcgctacacc
     8701 ggctccgagc cggattggga agcgctcgtg gacgtggaca ctgttgaatt tcagtcgctg
     8761 gatcccgtcc ggtcccggac gacgtggatt gccgttcacc acggtcggat cttcattccc
     8821 agcggctaca tgaccacctg gtggggtcga atctggaagc agtggcccat cgaagccgag
     8881 cgggacgggc gcgtgatcct gcgcgcggat ggcaagcttt acgatcggga gctcgttcgc
     8941 gtcagttcgg ggcccgagct cgagcctgtt ctggctgagc tgggccgcaa gtacggcgga
     9001 ggccaggcgt tcccgatgga cgccgtgacc aacggttacc tctggatatt cgagctcgca
     9061 cctccccggt agtcgctgct tcaggtcgcg tgtgcctcac aagcgaatcg cctgatagga
     9121 ttggcatccg cggagagcgg acgaaggcgg ggtttcaaac cggttgaatc ctgcttggag
     9181 tcggagaagg gagaagcgac aaacgtgatc aattgggacg ccatcggcgc catcggtgac
     9241 tt
//
LOCUS       DBNHOX010000584         2153 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_689535, whole genome shotgun sequence.
ACCESSION   DBNHOX010000584 DBNHOX010000000
VERSION     DBNHOX010000584.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_689535"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="AC16UZ_12840"
     CDS             <1..699
                     /locus_tag="AC16UZ_12840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6247614.1"
                     /translation="TMTDVLITGAARGIGFELARQYAEAGDRVYATSRNPDGAAQLSA
                     LAADFPDNVSVHRMDVGDMGSVQGCAAELNAAPIDVLINNAGVWGGLETQTFQNMDYE
                     NWAFEFNVMAMGPFRVIQTFLPNVLAGRDKKIVTLSSQVAAHVYDHVIGYSYASAKAA
                     VNRLMTALATELQDQGIIVVPMHPGWVKTDMAGDVADIYPSESAEGVRRVIAGMTMKD
                     TGKFLKWTGDIHPW"
     gene            769..1857
                     /locus_tag="AC16UZ_12845"
     CDS             769..1857
                     /locus_tag="AC16UZ_12845"
                     /inference="COORDINATES: protein motif:HMM:NF024375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6247615.1"
                     /translation="MNTGYNPEFNPTGIEGGIDTNALPWIDLPQAPGVSIKPLRASSE
                     SGMFSTVVRVADGSELSSIVYLGAMDMIVLSGRLTYPSGPMSGTLEPGTWGYVPANAR
                     VERFIAEQDTEFLANFYGPVAFLADDGRTVSSRLTSLDIQAAARDRGINLVPNTLAEC
                     MQERPPAYQGPVEPLAIAGSDSKALVVAAEGVAADAGRAVHPHFIDTRAVPWIVNPDT
                     PDLGLKILRVSEETGITSLIVRHNGVAGPHYHLGAADFMVLSGHIGYRAGPPEGYGPG
                     VWFYEPAGARHEATQRIGEEDLIYTANVYGPLQFDEGPDTPIAAVLSWMQYKAMADAG
                     GVDLVHSTFDNDASLLAWAPIGSEGARL"
     gene            1904..>2153
                     /locus_tag="AC16UZ_12850"
     CDS             1904..>2153
                     /locus_tag="AC16UZ_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247616.1"
                     /translation="MNKGIRVCEEIAASETDPRKRHNLEMVIAHMRAEARCDVQGVLD
                     TLVDEPCYVWHSAPDDPLLNPRGSREAVADFYDRMIVRT"
BASE COUNT          413 a          716 c          700 g          324 t
ORIGIN      
        1 acgatgacag acgttctgat caccggggca gcccgcggta tcggtttcga gctggccagg
       61 caatacgcgg aggccgggga ccgggtctac gccaccagcc gtaatcccga cggcgcagcc
      121 caactcagcg cgctggcggc cgatttcccg gacaacgtca gcgtgcaccg aatggacgtg
      181 ggcgacatgg ggtcggtgca gggctgcgcc gccgagctga acgcagcgcc catcgacgtg
      241 ctgatcaaca acgccggcgt ctggggcggg ctggagaccc agacttttca gaacatggac
      301 tacgagaact gggcgttcga gttcaacgtc atggccatgg gccccttccg ggtcatccag
      361 acctttctgc cgaacgtgct cgccgggcgc gacaagaaga tcgtgacgct ctccagccag
      421 gtagccgccc acgtctacga tcacgtcatc ggctactcct acgccagcgc caaggccgcc
      481 gtgaaccggc tgatgacggc cctggccacc gaactccagg accagggcat catcgtcgtt
      541 cccatgcacc cggggtgggt gaaaaccgac atggcgggcg acgtcgcaga catctatcct
      601 tcggaaagcg ccgagggcgt tcgccgggtc atcgccggga tgaccatgaa ggacacgggc
      661 aagttcctga aatggaccgg cgacattcac ccgtggtagc gacacgaacg gtttctgccg
      721 ggcgaccggc acacacgcaa tgcaaacaga tcagggaagg ctaaaccaat gaatacaggc
      781 tacaacccgg agttcaatcc caccggaatc gaaggcggca tcgacaccaa cgcgcttccc
      841 tggatcgacc tgccccaggc acccggggta tcgatcaagc cgctccgggc cagcagcgag
      901 tcgggcatgt tttccaccgt ggtcagggta gccgatggca gcgagctgtc gagcatcgtc
      961 tacctcggcg ccatggacat gatcgtcctg tcgggccggc tcacctaccc ctcgggaccc
     1021 atgtcgggga ccctggagcc cggaacctgg ggctacgtgc ccgcgaatgc ccgggtggaa
     1081 cggttcattg cggagcaaga cacggagttt ctggcgaact tctacggtcc ggttgccttt
     1141 ctcgcggacg acgggcgcac ggtgtcgagc cgactgacca gcctggacat ccaggcggcc
     1201 gcgcgcgacc ggggcatcaa cctggtgccc aacaccctgg cggagtgcat gcaggagcgg
     1261 ccgcccgctt atcagggccc cgtggagcca ctggccatcg ccggcagcga ctcaaaggca
     1321 ctggtggttg ccgccgaggg cgtcgctgca gatgcgggcc gtgcggtgca cccgcacttc
     1381 atcgacaccc gagcggttcc ctggatcgtc aatccggaca cgcccgacct gggcctgaag
     1441 atactccggg tcagcgagga aaccggcatc acgtcgctca tcgtgcggca caacggcgtc
     1501 gccgggcccc actaccacct gggcgccgcc gacttcatgg tgctcagcgg tcacatcggt
     1561 tatcgggcag gaccgcccga gggctacggg ccgggggtct ggttctacga gcccgccgga
     1621 gcgcgtcacg aagccaccca gcgcatcggc gaggaagatc tcatctacac cgcaaacgtc
     1681 tacggcccgc tgcagttcga cgagggcccc gacacgccca tcgcggccgt gctgtcctgg
     1741 atgcagtaca aggcaatggc cgatgccggc ggggtagatc tcgtgcacag caccttcgac
     1801 aacgacgcct cgctgctcgc ctgggcgccc atcggcagcg agggcgcccg gctctgagcc
     1861 gggcttcggc ggaagcaagc gggggatcga cgtgcggatg gacgtgaaca aaggcatccg
     1921 ggtctgcgag gaaatcgcag cctcggaaac cgaccccagg aagcgccaca atctggagat
     1981 ggtgattgcc cacatgcggg cggaagctcg ctgtgacgtc cagggcgttc tcgacacgct
     2041 cgtcgacgag ccatgctacg tctggcacag cgcgcccgac gatccgttgc tcaacccacg
     2101 cggcagcagg gaggcggtcg ccgacttcta cgatcggatg atcgtcagga ccg
//
LOCUS       DBNHOX010000585         4894 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1397013, whole genome shotgun sequence.
ACCESSION   DBNHOX010000585 DBNHOX010000000
VERSION     DBNHOX010000585.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1397013"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="AC16UZ_12855"
     CDS             complement(<1..884)
                     /locus_tag="AC16UZ_12855"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR6247617.1"
                     /translation="MTHVPTRFTTSLRTAIVLLAALMGSQCSASDASPDHPEAVILVY
                     HHVALGTPASTSVTPDRFAEHLGLLEHGGYAVIALQRVIAALERDEPLPPRSVALTFD
                     DAYRSVYTQALPALEARGWPFTVFVSTRAVDEGHANTMTWDELRDLEARGGAVGNHSR
                     SHDHLVRPRPGEKAEDWRARVIDDVLAAQSRLEEELERPLSVFAYPYGEFDLEIRNLI
                     RTLGFLAVGQQSGPVGTGTDPWAIPRFPVATGFDSPDRLREKLQTVALPVNVVAPSSN
                     TLTADAGPPELELVMSSG"
     gene            complement(894..1277)
                     /locus_tag="AC16UZ_12860"
     CDS             complement(894..1277)
                     /locus_tag="AC16UZ_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6247618.1"
                     /translation="MFTVLGLDHVVIRASDPGKLIGFYQEVLGCRLERELPELGLFQL
                     RAGRSLIDVLARPDAAEPCEPNMDHFCLRVEPFDVQSLARHFEGLDIPARIATRVYGA
                     EGYGPALYIQDPEGNTVELKGPAGG"
     gene            1398..2252
                     /locus_tag="AC16UZ_12865"
     CDS             1398..2252
                     /locus_tag="AC16UZ_12865"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6247619.1"
                     /translation="MNNHSPVFDVQPQSFQADVVERSQQVPILLLFWAEQVPPSADAR
                     RELEALVAQYQGKALLGLVDVAQDPTLAQHLRVQGVPSIRVIKGGQLVDQLDGPQPEH
                     VLRALLDQLTLSPADQLHDQLQLLLGSGDFQSALQLLQQAINEEPNNAGFRVELADVL
                     VLKGELDEARKVLAGIAKDADDIDRPQIRLEMAEEAAGMPSMSELAEAHQRDPEDLET
                     RYGLAVVGATAGNYEFALEHAMAILQQDREFRDDIGRTTMIRIFSLLGKGSDLAQRYR
                     RKMFAFMH"
     gene            2398..2730
                     /locus_tag="AC16UZ_12870"
     CDS             2398..2730
                     /locus_tag="AC16UZ_12870"
                     /inference="COORDINATES: protein motif:HMM:NF012708.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR6247620.1"
                     /translation="MEAHDGSQLLLAWQDGRVTLTGHDQVLVLGAAQSADVPVRGNFA
                     SRNHARIERRKQEYVLIDHSTNGTFVQTEDERVTFVRRREVRLWGAGWISLGEPLSPA
                     TAIHFENV"
     gene            2736..3458
                     /locus_tag="AC16UZ_12875"
     CDS             2736..3458
                     /locus_tag="AC16UZ_12875"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6247621.1"
                     /translation="MELSWQSAEACHCGKRRASNDDAVVSRPSARLWAVADGMGGHKA
                     GDVASGAIADALGRLTLNGNLADDVDAVDDALGEVNEQLREHARETCPGATVGSTVVA
                     MLALAGVGVALWAGDSRLYRLRGRRLEQITRDHNPVSDLLDSGAVSESEAVAADTNIV
                     TRAVGGHSELFLDVAVFDIDPQDTYLLCSDGLYRELNTADLAHGLQGESPAEIANGLL
                     SRCLETAARDNVSLIVARPDVT"
     gene            3455..>4894
                     /locus_tag="AC16UZ_12880"
     CDS             3455..>4894
                     /locus_tag="AC16UZ_12880"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6247622.1"
                     /translation="MSRPAHQDATVIRADPSLSVSFAGEGVGETNAPRILKQRFVLEE
                     KLGSGGMGTVFRAKDLRKVEARDRQPYLAIKVLNDNFRAHPEAFIALQREAAKSQAVS
                     HANIVSIYDYDKDGDVPFITMELLEGQELADLLRAYPNGLPDPMAWEVIRGICAGLGH
                     AHSTGVVHADFKPGNVFVSPRNAAKVLDFGIARAVRLNQIYGEETVIDPVRLGALTPA
                     YASREMLQGDNPEPRDDIYALGVVIYLVLTGHHPYGRTPADEAAREGLAPDRPRRLTR
                     RQWRVLEKCLRFNRHERPATIDEVEHYLLKPAPWRSRTALAAAAAFALSLGINYLVGD
                     AELTEVREEVRQSTLVDAQTARVQAMLSEPDFEPGWEDRIASELDRLNALDESGASGA
                     RMSAQIIALLTQRIVATRDLGNAFELYRSATRFGEPERAGRVLHDRLLARVFALLESP
                     QPEQRWFDSVRRELARLASTFPESKEIEPI"
BASE COUNT          843 a         1587 c         1654 g          810 t
ORIGIN      
        1 ctgccggagc tcattaccag ctcgagctcg ggaggcccgg cgtcggctgt aagcgtgttc
       61 gagctcggag caacgacgtt cacaggcagg gcaacggttt ggagcttctc cctgagccga
      121 tccggcgagt cgaagccggt ggccaccggg aagcggggta ttgcccacgg atcggtcccg
      181 gtgcccacgg gtcccgactg ctgcccgacc gcgagaaaac cgagcgttcg gattagattc
      241 ctgatctcca gatcgaactc gccgtaggga taggcgaaca ccgacagggg tctttcgagc
      301 tcctcctcga gtcggctttg ggcagcaagg acgtcgtcga tcacccgcgc gcgccagtct
      361 tccgccttct cccccggccg cgggcgaacc agatgatcgt gggagcggga gtggttgccc
      421 accgccccgc cccgggcttc gaggtccctc agctcatccc atgtcatggt gtttgcatga
      481 ccctcgtcga ctgcccgggt gctgacgaaa acggtgaacg gccatcctcg agcttccaga
      541 gcggggaggg cctgggtata gaccgaccgg taggcgtcat cgaaggtgag cgcgacgctg
      601 cgcggtggga gcggctcgtc ccgctccagg gcggcgatca cccgttgcag cgcgatgacg
      661 gcataccccc catgctcgag cagcccgagg tgctctgcaa agcgatcggg cgttacgctg
      721 gtgctggcgg gcgtgcccag cgcgacgtgg tggtagacga ggattacggc ttcggggtga
      781 tcgggggaag cgtcggaagc cgagcactga gaccccatga gcgcggccag caggacgatt
      841 gccgtgcgca ggctcgtcgt gaagcgggtg ggaacgtgcg tcacgccagg gcctcagccg
      901 cccgccgggc ctttgagctc gacggtattg ccctccggat cctgtatgta gagcgccgga
      961 ccgtaacctt cggcgccgta cacccgggta gcgatccggg cagggatgtc caggccctcg
     1021 aaatgccggg ccagcgactg cacgtcgaac ggctcgacgc gcaggcagaa gtgatccatg
     1081 ttgggctcgc agggctcggc tgcgtcgggt cgggcgagca cgtcgatcag cgaacgtccg
     1141 gcccgcagct gaaaaaggcc caactcgggc agctcacgct cgagcctgca tcccagcact
     1201 tcctggtaaa agccgatcag cttgccggga tcgctggcgc ggatcaccac gtggtcgagg
     1261 ccgaggacgg tgaacatggc tgatgcttcc tggggcatgg ctgccattgt agtgcgcttg
     1321 agggcgggtt gctcacgtcc tccccgtgtg ctggtgtatg atgcccggct gttcaggaac
     1381 gacccagacg ctttcagatg aacaatcact ccccggtatt cgacgttcag cctcagtcgt
     1441 ttcaggcaga cgtggtggag cgctctcagc aggtgcccat cctgctgctc ttctgggcgg
     1501 agcaggtccc cccatccgct gacgccagac gcgagctgga ggcgttggtc gcgcagtatc
     1561 agggcaaggc gctgctcggt ctggtggacg tggcccagga tcccaccctg gcccagcacc
     1621 tgcgggtaca gggcgtgccg agtatccgcg tgatcaaggg cgggcagctc gtcgatcagc
     1681 tcgacggtcc gcagcccgag cacgtcctgc gggccctgct ggatcagctc acgctatccc
     1741 cggccgacca gctgcacgac cagctgcagc tgctcttagg cagcggcgac ttccagtctg
     1801 ccctccagct gctgcagcag gccatcaacg aggaaccgaa caacgccgga ttccgcgtcg
     1861 agcttgccga cgttctcgtg ctgaaaggcg agctcgacga ggcgagaaaa gtgctcgcgg
     1921 gaattgccaa ggatgctgac gacatcgacc gcccgcagat tcgcctggag atggccgaag
     1981 aggctgccgg catgccgtcc atgagcgagc tggccgaggc gcaccagcgc gatcctgagg
     2041 atctggaaac ccgctacggg ctagccgtgg tcggcgccac cgccggcaac tacgagttcg
     2101 ccctcgagca cgccatggcc atcctgcagc aggatcgaga gtttcgggac gacatcggcc
     2161 ggacgaccat gatcaggatc ttctcgctgc tgggcaaagg ttcggatctc gctcagcggt
     2221 atcgacgcaa gatgtttgcc ttcatgcact gaccgggcgt tgcccgtctc tttgcccctg
     2281 tcaccgcgcc ggcgctcgtg aatagcgccg tcggcgggcg ctttgtgatc cagtcctcat
     2341 ccctgcaaag gccgctcacc tatccttgac gggatatgta ggcccggtga ctgcgacatg
     2401 gaagcgcatg acggttctca gctgctcttg gcgtggcagg acggccgcgt caccctgaca
     2461 ggccatgacc aggtactggt ccttggtgcc gcccagagcg ccgacgttcc cgtgcgcggc
     2521 aattttgcct cgcgcaacca tgcccggatc gaacgccgca agcaggagta cgtcctcatc
     2581 gatcactcca cgaacggcac gttcgtgcag accgaggacg agcgggtcac ctttgtcagg
     2641 cgccgggaag tgcgtctgtg gggggccggc tggatcagcc tcggcgagcc tctcagtccc
     2701 gcgacggcca ttcacttcga gaacgtttag gggtcatgga actcagctgg caaagcgccg
     2761 aagcgtgtca ctgcggcaag cggcgggcga gcaacgacga cgcggtggtg agccggccgt
     2821 cggcgcggct ctgggccgtg gcggatggga tgggcggtca caaggcgggc gacgtcgcca
     2881 gcggcgccat agccgatgcg cttggcaggc tgacgctcaa cggcaacctg gccgacgacg
     2941 tggatgcggt cgatgatgcc ctcggcgagg tcaacgagca gcttcgcgag catgcgcggg
     3001 aaacctgtcc cggcgccacg gtgggcagca ccgtggttgc gatgctcgcg ctggcaggcg
     3061 tgggcgtggc gctatgggcg ggcgacagcc gcctctaccg gctgcggggt cgccgcctgg
     3121 agcagatcac ccgggatcac aacccggtct ccgacctgct ggacagcggt gccgtctccg
     3181 agtcggaagc cgttgccgca gatacgaaca tcgttacccg ggcggtgggc ggacactccg
     3241 agctgttcct ggacgtggcg gtctttgaca tcgaccccca ggatacctac ctgctctgca
     3301 gtgatggcct gtaccgcgag ctgaacacgg cggacctggc tcatgggctt cagggcgagt
     3361 cgccggctga gatcgcaaac ggcctgctca gccgctgcct cgagacggcc gctcgggaca
     3421 acgtgtcgct gatcgtcgcg aggcccgacg tcacgtgagc agacccgccc atcaggacgc
     3481 caccgtcata cgggcggatc cgagcctttc cgtcagcttc gcgggcgagg gagtcggtga
     3541 gaccaacgcc ccccggattc tcaagcagcg attcgtgctc gaggagaagc tcggcagcgg
     3601 aggcatgggc acggtgttcc gggcaaagga cctgcgcaaa gtggaagctc gcgaccggca
     3661 gccctacctg gccataaagg tgctcaacga caacttccgc gcccatccgg aggcgttcat
     3721 cgcccttcag cgggaagcgg ccaagtccca ggcggtgtct cacgccaaca tcgtgtccat
     3781 ctacgattac gacaaggacg gcgacgtccc cttcatcacc atggagctgc tggaggggca
     3841 ggaactcgcc gacctcctgc gcgcgtaccc gaacgggctt cccgatccca tggcctggga
     3901 ggtcatccgc ggcatctgcg ccgggctcgg ccacgctcac agcacgggcg tggtacacgc
     3961 ggacttcaaa cccggaaacg tgttcgtctc gccgcgcaat gccgcaaagg ttctcgactt
     4021 cggcatcgcc cgggccgttc ggctcaatca gatctacggt gaagagacgg tcatcgatcc
     4081 ggttcgtctg ggtgcgctga cgccggcgta cgcaagccgc gagatgctgc agggggacaa
     4141 tccggagccc cgggacgaca tctacgcgct cggggtggtg atatacctcg tcctgaccgg
     4201 gcaccatccc tacgggcgca cgccggccga cgaagccgcc cgcgagggtc tcgctccgga
     4261 caggcccaga cggctcaccc ggcggcagtg gcgtgtgctg gaaaagtgcc tgcgcttcaa
     4321 tcgccatgag cgaccggcga ccatcgacga ggtcgagcac tatctgctca aaccggcgcc
     4381 gtggcgttcg cgcacggcgc tcgccgccgc agcggcattc gccctctcgc tgggtatcaa
     4441 ctacctggtg ggcgatgcgg agctcaccga ggtcagggag gaggttcggc agtcgacgct
     4501 ggtggatgcg cagacggctc gggtccaggc gatgctcagc gagccggatt tcgagccggg
     4561 ctgggaagac cggatagcca gcgagctgga tcggctgaac gccctggacg aatccggcgc
     4621 aagtggcgcc cggatgagtg ctcagatcat cgcgctgctc acgcaacgga tcgtcgcgac
     4681 tcgggacctg ggcaatgcct tcgagctcta ccgcagcgca acgcgattcg gcgagcccga
     4741 gcgggcaggc agggtccttc acgaccgcct gctcgccaga gtcttcgcgc tgctcgagtc
     4801 gccgcagcct gagcagcgct ggttcgactc cgtgcgaagg gagcttgcgc ggctggcctc
     4861 aacctttcct gagagcaagg agatcgagcc gatc
//
LOCUS       DBNHOX010000586         2116 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1075639, whole genome shotgun sequence.
ACCESSION   DBNHOX010000586 DBNHOX010000000
VERSION     DBNHOX010000586.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1075639"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..786)
                     /locus_tag="AC16UZ_12885"
     CDS             complement(112..786)
                     /locus_tag="AC16UZ_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247623.1"
                     /translation="MATVTIRQSFPNTFQVAGGQTTGAGNITGLAANKGILMLAAWSG
                     TVLLPPANITLGGVNPTAGGNFITVSSQRLRWYWWENNTQTGTQSLSVTFSAALSTGG
                     AKISVFELNTPIRAGNSTGGADPYTFTLALADPSVFVSIIGSGALTAPAGGYTVALND
                     TAINTVNHEIDYRLTGNTGGSVVFDGTFSQAVGVEVELVALGTEGLVNDAVNDVVSDV
                     LEDLAQ"
     gene            complement(790..1050)
                     /locus_tag="AC16UZ_12890"
     CDS             complement(790..1050)
                     /locus_tag="AC16UZ_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247624.1"
                     /translation="MATGTLSADGDTSEHIGEKVHVHLSGTFGSGTLTLYFKDENGNW
                     KAITNGAFTAAADKVLNFPFPTAVKGTLSGATAPSLYYNLRG"
     gene            complement(1060..1284)
                     /locus_tag="AC16UZ_12895"
     CDS             complement(1060..1284)
                     /locus_tag="AC16UZ_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247625.1"
                     /translation="MSHPFPSQIRVFSDHPHNLHGTWKLYPTAEGTRYVRGDIFEKFR
                     QFIADNVEHEEDCATERDHECDCKLSDYLF"
     gene            complement(1323..>2116)
                     /locus_tag="AC16UZ_12900"
     CDS             complement(1323..>2116)
                     /locus_tag="AC16UZ_12900"
                     /inference="COORDINATES: protein motif:HMM:NF049598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HIR6247626.1"
                     /translation="EMLVRILAETGFKHLMLKVHRLLRTNQDRAQAFKLRGEWVEVDP
                     SEWLDRPNVKVNVGLGFHNKEQRVQMLFQLLQLQKEALQLGLSDPPKIYNLLDQLVEA
                     AGLGNAQDYFVDPGKPGYQPPQPPPDPMAEAAQAQMQVEQGKLQKDMQKLQGDFQIEM
                     QKLQGYLGEIQARIGQIEADTKLKEAQTAEVWARIGKLHKDDARQDRGEERADHKEAF
                     NIAQGLRGAKVEDDDREYNRASEAENRQRQRELEDREFQRGLQEE"
BASE COUNT          311 a          700 c          678 g          427 t
ORIGIN      
        1 gcacgatcct cacccggagc ccgagccaga tgaacccatc aacgcgatcg acccggcgcc
       61 aggagaaccc ggcccgggtg atgtagatct tgcgcttctt ggtgcggatc ttcattgggc
      121 caggtcctcg agcacgtcgc tcaccacgtc attcacggcg tcgttcacca ggccttcagt
      181 gcccagggcc acaagctcga cctccacgcc caccgcctgg gagaacgtgc cgtcgaagac
      241 caccgagccg ccggtgttgc ccgtcagccg gtagtcgatc tcgtggttga cggtgttgat
      301 cgcggtgtcg ttcagggcga ccgtgtagcc gccggcagga gccgtcagcg cgcccgagcc
      361 gatgatcgac acgaacacgc tggggtcggc cagggccagg gtgaaggtgt agggatccgc
      421 cccgccggtg ctgttgccag cccgtatggg cgtgttcagc tcgaacaccg atatcttggc
      481 gccgccagtg ctcagggcgg ccgagaacgt caccgacagc gactgggtgc cggtctgggt
      541 gttgttctcc caccagtacc aacgcaggcg ctggctcgac acggtgatga agttgccccc
      601 ggcggtgggg ttgacacctc ccagggtgat gttggcaggc ggcagcagga ccgtgcccga
      661 ccaggccgcg agcatcagga tgcccttgtt cgcagccagg ccggtgatgt tgcctgcgcc
      721 cgtggtctgg ccacccgcta cctggaaggt gttcgggaag ctctgcctaa tggtgacggt
      781 cgccacctat cagcccctca ggttgtagta caggctcggg gccgtcgccc ctgacagcgt
      841 gcccttgaca gcggtcggga acgggaagtt caggaccttg tccgccgccg ccgtgaaggc
      901 gccgttcgtg atggccttcc agttgccgtt ctcgtccttg aaatacaggg tgagcgtgcc
      961 actcccgaag gtgccgctca ggtgaacgtg gaccttctcg ccaatgtgct cactcgtgtc
     1021 gccgtctgcg ctcagggtgc ctgttgccat gtcgtctcct caaaataggt agtcgctcag
     1081 tttgcaatcg cactcgtggt cgcgctcggt ggcgcagtcc tcctcgtgct ctacgttgtc
     1141 cgcaatgaac tgccggaact tctcgaagat gtctccccgg acgtacctgg tgccctccgc
     1201 ggtagggtac agcttccagg tgccgtgcag gttgtgcggg tggtcgctga agactcggat
     1261 ctggctcggg aacgggtggc tcatggtatt ctcctcaggg atggggagag tctaccacgc
     1321 cctcactcct cctgcaagcc ccgctggaac tcgcggtcct ctagctcgcg ctgccgctgg
     1381 cggttctcag cctccgaggc tcggttgtac tcccgatcgt cgtcctcgac cttggcgcca
     1441 cgcagcccct gggcgatgtt gaaggcctcc ttgtgatccg cgcgctcctc cccacggtcc
     1501 tggcgggcgt cgtccttgtg cagcttgccg atgcgagccc acacctcggc ggtctgggcc
     1561 tccttgagct tggtgtcggc ctcgatctgc ccgatgcgcg cctggatctc gcccaggtag
     1621 ccctggagct tctgcatctc gatctggaag tcgccctgga gcttctgcat gtccttctgg
     1681 agcttgccct gctccacctg catctgagcc tgggcggcct cggccatcgg gtcaggcggc
     1741 ggctgcggcg gctggtagcc gggcttgccg gggtcgacga agtagtcctg ggcattcccc
     1801 aggcccgcgg cctccacgag ctgatcgagc aggttgtaga tcttgggcgg gtcggacagc
     1861 ccgagctgca gggcctcctt ctggagctgc agaagctgga acagcatctg tacgcgctgc
     1921 tccttgttgt ggaagccaag gccgacgttc accttgacgt tggggcgatc cagccactcg
     1981 ctcggatcca cctccaccca ctcaccacgc agcttgaacg cctgggctcg gtcctggttc
     2041 gtccgcaaca ggcggtggac cttgagcatc aggtgcttga acccggtctc ggccaggatc
     2101 cgcacgagca tctcga
//
LOCUS       DBNHOX010000587         2216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_46547, whole genome shotgun sequence.
ACCESSION   DBNHOX010000587 DBNHOX010000000
VERSION     DBNHOX010000587.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_46547"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="AC16UZ_12905"
     CDS             complement(<1..329)
                     /locus_tag="AC16UZ_12905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6247627.1"
                     /translation="MSLETVDFARLKLAPGDNVLDLGCGEGRHAITAYLREAVTVVGM
                     DLSLGDLTTARQRYGEFRDGDDASRRVDFILATGHNLPFADATFDKVICAEVLEHIHD
                     YENVLGE"
     gene            complement(326..1600)
                     /locus_tag="AC16UZ_12910"
     CDS             complement(326..1600)
                     /locus_tag="AC16UZ_12910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6247628.1"
                     /translation="MSELATYEFGQEAPSGRRPLRIALLGYRSNPFSGGQGVYLKFLS
                     RALVDAGHEVDVISGEPYPELDGRVGLIKLPGLNLFDVPNHVTALRPKHLLSATDFFE
                     WLSMLSGGFPEPYTFGRRLTRYFSRIDDRYDVVHDNQSLCYGLLNVARRTPVICTIHH
                     PITWDRDIAIANAATRGERLLIRRWHSFLAMQTRVAKRLPNIVTVSERSKRDICAAFE
                     IPQGRVTVVRNGIDTEAFRPDPTVTRKPWQLVTTASADQPLKGAQHLLPAFAQLCRRF
                     PELRLVFIGAPRPGGKTDRLVDALGVRHRMDFIHGISTADIRRLYAESTVAVVPSEYE
                     GFGLPAGEAMACGTPLVSTDGGALPEVVGQAGRIVPSRDPDALAEAIAGLLVDPKERE
                     ALSRAGRARMVEHFAWAEAAERLTALYEQVLA"
     gene            complement(1680..>2216)
                     /locus_tag="AC16UZ_12915"
     CDS             complement(1680..>2216)
                     /locus_tag="AC16UZ_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247629.1"
                     /translation="SPPADATDAWMMPYHVTRGDLFVRQHYADHPLIDVDRVAAYIEM
                     TRFPVPDRPPYQQLETLGALVRAADLIGQMGDPNYLAKLSSLFVEFKENGEADRLGFA
                     SVAELRADSQAFFSQQISPYLPKALKLLRATEEGRAWVVSLYENIERRAQAGGSAAQP
                     DPSKQENSRAGRIHAGNG"
BASE COUNT          378 a          689 c          764 g          385 t
ORIGIN      
        1 atctcaccga gaacgttctc gtagtcgtgt atgtgctcga gaacttccgc gcagatcacc
       61 ttgtcgaagg tggcgtcggc gaacggcagg ttgtgcccgg tggcgaggat gaagtccacc
      121 cgccgagagg cgtcgtcgcc atcgcggaac tcgccgtagc gttgccgcgc ggttgtcaga
      181 tcgccgagcg acagatccat gccgacgaca gtgacggcct ccctgaggta ggcggtgatg
      241 gcatggcggc cctctccgca gccaagatcc aggacgttgt ccccgggggc gagtttcagg
      301 cgcgcgaaat cgacggtctc cagactcatg cgagcacctg ctcgtagaga gcggtgagcc
      361 gctctgcggc ttctgcccag gcaaagtgtt cgaccatgcg ggcccggccg gcgcggctga
      421 gcgcttcgcg ctccttcggg tcgaccagaa gcccagcgat ggcctctgcc agcgcgtccg
      481 ggtcgcggct cggcacgatc cggcccgcct gaccgacaac ctctggaagc gcgccgccgt
      541 ccgtgctcac cagtggcgta ccgcaggcca tcgcctcgcc ggccggtagt ccgaagccct
      601 catattccga cgggacgacg gccacggtgg attcggcata gagcctccgg atgtctgccg
      661 tgctgatccc gtgaatgaaa tccatgcgat ggcgaacgcc gagcgcgtct accaggcgat
      721 ccgttttgcc cccgggacgc ggggcgccta tgaagactag gcgcagctcg ggaaagcgcc
      781 ggcacagctg ggcgaacgcg ggtagcaggt gctgggcgcc cttgagtggc tgatcggcac
      841 tcgccgtggt gacgagttgc cagggtttgc gcgtcacggt cggatccggg cggaacgcct
      901 cggtgtcgat gccgttcctg acgacggtca cccggccctg ggggatctcg aaagccgcgc
      961 agatgtcgcg cttggaccgc tcgctgacgg tgacgatgtt gggcagacgc tttgccacgc
     1021 gggtctgcat ggcaagaaag ctgtgccagc gtcggatcag cagccgctcg ccccgggttg
     1081 cggcgttggc gatggcgata tcgcggtccc aggtgatggg atggtgaatg gtgcagatca
     1141 ccggcgttcg gcgcgcgacg ttcagcaggc cgtagcacag gctctggttg tcgtgtacga
     1201 cgtcgtagcg atcgtcgatt cgggagaagt atctcgtcag gcgtcgcccg aaggtatacg
     1261 gctccgggaa tccgccgctc agcatggaca accattcgaa gaagtcggtt gccgaaagga
     1321 gatgcttcgg gcgcagcgcg gtcacgtgat tgggcacgtc gaacaggttc aggcctggca
     1381 gcttgatcag cccgacgcgc ccgtccagtt ccgggtaggg ctcgccggat atcacgtcca
     1441 cctcgtgccc ggcatccacc aaggcgcggg agaggaactt cagatacacg ccctgcccgc
     1501 cactgaacgg gttgctgcga taccccagca gcgcgattcg cagtggccgc cgaccagaag
     1561 gcgcttcctg gccgaactcg taggtcgcga gctccgacat gcctgccccg agcaaagcgg
     1621 acaagtctaa tcagcggcgc gaaggagtgc caggcgttgg acggcggatg agggtagggt
     1681 cagccgttgc ccgcgtggat acggccagcg cgggaattct cctgctttga tgggtcaggc
     1741 tgggccgcag agccccccgc ctgggcccgc cgctcgatgt tctcgtacag gctgacgacc
     1801 caggcccggc cctcttcggt tgcgcgaagc agcttcagcg ctttaggcag gtagggtgaa
     1861 atctgctggc tgaaaaaagc ctgggaatcc gcgcgaagct cggccacgct cgcgaacccg
     1921 agccggtcag cctcgccgtt ttccttgaac tccacgaaca ggctggacag ttttgccaga
     1981 tagttcgggt cgcccatctg gccgatcaga tcggctgccc gcacgagcgc gcccagggtt
     2041 tccagctgct ggtagggtgg ccggtcggga accgggaagc gcgtcatttc gatgtaggcc
     2101 gccacgcgat cgacgtcgat cagcggatgg tctgcatagt gttggcgaac gaagagatca
     2161 ccgcgggtga cgtggtacgg catcatccag gcatctgtgg cgtccgcggg gggcga
//
LOCUS       DBNHOX010000588         4698 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_689956, whole genome shotgun sequence.
ACCESSION   DBNHOX010000588 DBNHOX010000000
VERSION     DBNHOX010000588.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_689956"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="AC16UZ_12920"
     CDS             complement(<1..1524)
                     /locus_tag="AC16UZ_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR6247630.1"
                     /translation="MTEATLNYGDVTQLAALDLGSNSFHLIVAQETNGRLQIIDKIKE
                     MVRLANGLDEKNELTGPATDTALACLERFGQRLNSLQRRNVRVVGTNTLRRARNAAAF
                     IERAEQALGHPVEIISGREEARLIYLGVSHSIEDNYERRLVIDIGGGSTELILGRHFE
                     AEKTESLHMGCVGISRQFFGDGRIRAGQFRAAVNHARKELVPVAGTYGRKSRDTVIGA
                     SGTILAAQAVLAEMDGSRDISLDGLERVREALIDAGHVDKLALPGLPPERAPVFPGGL
                     AVLIAAFKQLDIASMEATSGALREGLLYDLLGRVHHQDVRENSVADLCMRYHVDQTHA
                     RRVREMALALLAQVALEWDLTEPRHQLLLGWAADLHEIGMDIAHSQYHRHGGYLLANM
                     DLPGFSKGDQLELAALVRAHRRKFQQEDGDYPPHALRLAVLLRLAVLLHRNRANTALP
                     HVALRADGDALLLKFPRGWLKRHPLTRLDLDQEADYRSAVGVGLRIDENENAARSVAG
                     "
     gene            complement(1599..3539)
                     /locus_tag="AC16UZ_12925"
     CDS             complement(1599..3539)
                     /locus_tag="AC16UZ_12925"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6247631.1"
                     /translation="MNFFRAQDDAKRKTWRLALLFAAAVGSLIVLTNLLLMGVYRWTT
                     DVGVATSPDATAAFTNVPTEQWLIVTAAVVAVVGIASLYKYLVIRDGGKTIAEALGGR
                     RINANTTDPAERRLLNVVEEMAIASGTAVPPVYLIPEHSINAFAAGFTPDDAVIGINQ
                     GTLDQLNRDELQGVVGHEFSHILNGDSRINLRLIAMLHGILFLGIIGYGILRGMAHGG
                     NRRSGGNAAVPVMALGLGLLIIGYSGTFFGNLIKAAVSRQREYLADAAAVQFTRNPSG
                     LANALKRIGGLPVGSTMSGSNAREISHMFFGQCSRPFFSGLMSTHPPLEDRIRAIEPR
                     WDGRLLERGQPSPTRPAGAAGFAGTGAAGIQVPGGISVGVPAESIADEVGRLDQTSLD
                     NAVGIIASIDPKLREAAHDPWSGRGLIYATLTHADGYARDRQLAYLEENAEPGVPEHV
                     FRLVPAVRHLDEPRKLALVEMAVPALKELSDPQFRRFIDNVVAMIRSDRQIDLFEWVM
                     HRLLIKELRPHFDGPERIRARYRSPDQVADAALTVLSALARSGHGDVGAQAKALATGA
                     ELLDLAPRMDTEDDPNFVRLGQALWQLRHLKPLVKPRVLKACAAVALADGQVSGREGA
                     LLQGIAATLDCPLPPSIYAPKS"
     gene            complement(3556..4149)
                     /locus_tag="AC16UZ_12930"
     CDS             complement(3556..4149)
                     /locus_tag="AC16UZ_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009043301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR6247632.1"
                     /translation="METIIVLVIIAALAFWVIAIYNRLVTLKNRFKNAFSQIEVQLKR
                     RYDLIPNLVETAKAYMSHERETLEAVIAARNQALAGLQTAAQDPGNADAIRQLASAEG
                     ALGGALGRLNVVMEAYPDLKANQNMMQVSEELTSTENRVAFARQAYNDQVTDYNTYKQ
                     SFPAMLLAGPFGHAGDAALLEYEDSEAMQEAPKVSFS"
     gene            complement(4192..>4698)
                     /locus_tag="AC16UZ_12935"
     CDS             complement(4192..>4698)
                     /locus_tag="AC16UZ_12935"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6247633.1"
                     /translation="LVGGLNKGWSIAKRLLQHERQMVAGIGGMSALGGTGRRLEDVAK
                     AYVGEAQGRIGDPAVRSSVIEHRMNDRAFQLTLERSGDEAKAGQADGNLASMFKYYGT
                     EQNKRKYELMLQAMGTQSMGWEGEAFSAKELGTTRQWLRSKANSIEGGTSEVQLNVIA
                     KRVLGLPD"
BASE COUNT          727 a         1538 c         1568 g          865 t
ORIGIN      
        1 gccggcgact gatcgcgcgg cgttttcgtt ctcgtcgatg cgcagaccga ctccgaccgc
       61 cgaccggtag tcggcttcct ggtccagatc caggcgggtg agcgggtggc gtttgagcca
      121 gccgcgggga aacttcagca acagcgcgtc tccgtctgct cgcagcgcga cgtgagggag
      181 cgccgtgttg gcgcggttgc ggtgcaggag cacggccagt ctcagcagca ccgctagcct
      241 cagggcatgg gggggataat cgccgtcttc ctgctggaac ttgcgccggt gggctcggac
      301 cagcgcggcc agctccagct ggtcgccttt ggagaagccc ggaaggtcca tgttggcgag
      361 caggtagccg ccgtgcctgt ggtactggct gtgcgcgatg tccatgccga tctcatgcag
      421 atcggccgcc caccccagca ggagctgatg tctcggctcg gtgagatccc attcgagggc
      481 tacctgggcc aggagggcca gcgccatctc cctcacccgg cgcgcgtgtg tctggtcgac
      541 gtgatagcgc atgcacaggt ccgcgacgct gttctcccgc acgtcctggt ggtgcacccg
      601 acccagcaga tcgtagagca gcccttcgcg gagagcgccg ctggtagcct ccatgctcgc
      661 gatgtcgagc tgtttgaagg cggcgatgag cacggcaagg cctcccggga atactggcgc
      721 ccgctcgggg ggcagaccgg gcaaagccag cttgtccacg tggccggcat cgatcagggc
      781 ctcgcgcacg cgttccagac catcgagcga aatgtccctg ctcccgtcca tctctgcaag
      841 cacggcctga gcggccagga tggtacccga ggcgccgatc accgtgtccc gggactttct
      901 gccgtaggtt ccggccaccg gaacgagctc ttttctggcg tgattgacgg ccgctcgaaa
      961 ctgtccggcc cggatacgtc cgtcgccgaa gaactgtcgc gagatcccca cgcatcccat
     1021 gtgcaggctc tcggttttct ccgcctcgaa atggcggccg aggatgagct ccgtgctgcc
     1081 gccgccgatg tcgatgacca ggcggcgttc gtagttgtcc tcgatggagt gagatacgcc
     1141 cagatagatc aggcgggcct cctcgcgccc ggagatgatc tccaccgggt ggcctagagc
     1201 ctgctcggcc cgttcgatga aggcggccgc gttgcgcgcg cgcctcaagg tattggtccc
     1261 cacgacgcga acgttgcggc gctgcaggct gttcagccgc tggccgaacc gctcgaggca
     1321 ggcgagcgct gtatccgtgg cgggcccggt cagctcgttc ttctcgtcaa ggccgtttgc
     1381 gagcctgacc atctccttga tcttgtcgat gatctgcagc cgcccgttgg tttcctgagc
     1441 gacgatgaga tgaaagctgt ttgagcccag gtccagtgcc gcgagctggg tgacgtcgcc
     1501 gtagttgaga gtggcctcgg tcatggggcc cagtgcggat cgaaagcgtc attctataga
     1561 gcaaggtcgg acgcgcaagc gggggcgctt gctccctctc aggacttcgg agcgtagatc
     1621 gagggcggta acgggcaatc cagcgtcgct gcgatgccct gcagcagggc gccctctcgg
     1681 ccgcttacct ggccgtcggc aagcgctacg gcagcgcagg ctttcagcac gcgcggcttc
     1741 accagcggct ttagatggcg aagctgccaa agggcctgac cgagacggac gaagttgggg
     1801 tcgtcttctg tgtccattcg aggggcaagg tcgagcagct cggcgccggt cgccagggcc
     1861 ttggcctgag ccccgacatc cccatgaccg gaacgtgcca gcgcggaaag caccgtcagc
     1921 gctgcatcgg ccacctggtc gggggagcgg tagcgggccc tgatccgctc cgggccgtcg
     1981 aagtgcggac gcagctcctt gatcagcagc ctgtgcatga cccactcgaa caggtcgatt
     2041 tggcgatcgg acctgatcat cgcaaccaca ttgtcgatga accgcctgaa ctgggggtcg
     2101 gacagctctt tcaatgccgg caccgccatt tccaccagcg cgagcttgcg gggctcgtcg
     2161 agatgcctga ccgcgggaac caggcgaaag acgtgttcgg gcacgccggg ttccgcgttt
     2221 tcctcgagat aggcgagctg tcggtcgcgc gcatagccat cggcgtgggt gagggtcgcg
     2281 tagatcagcc cgcgaccgga ccaggggtcg tgagcagctt cgcgcagctt cggatcgatg
     2341 ctcgcgatga tgcccaccgc gttgtccagg ctcgtctggt ccaggcggcc gacctcgtcc
     2401 gcgatgctct ccgcggggac ccccaccgag atgccccccg gtacttgaat gcccgctgcg
     2461 ccggtacccg caaatcccgc ggcgccggca gggcgcgtcg gggacggttg gccacgttcc
     2521 aggagccgac cgtcccagcg cggttcgatg gcgcggatcc ggtcttcgag ggggggatgg
     2581 gtagacatca gaccgctgaa aaacggccga gagcactggc cgaagaacat gtggctgatc
     2641 tcacgggcat tcgatcccga catggtcgac cccacgggca ggccgccgat gcgcttgagc
     2701 gcattggcca ggccgctggg gtttcgcgtg aactgcaccg ccgcagcatc tgccagatac
     2761 tcgcgctgtc ggctcaccgc ggccttgatg agattgccga agaaggtgcc cgagtagccg
     2821 atgatcagca gccccaggcc gagggccatc accggcaccg ccgcgttgcc cccggatcgg
     2881 cggttgccgc cgtgggccat gccccggagg atgccgtaac cgatgatgcc gaggaagagg
     2941 atgccgtgca gcatcgcgat caggcgcagg ttgatgcggc tgtcgccgtt caagatgtgg
     3001 ctgaactcgt ggccgaccac cccctgaagc tcgtccctgt tcagctggtc aagggtgccc
     3061 tggttgatcc cgatcacggc gtcatcgggc gtgaatccgg ccgcgaaggc gttgatgctg
     3121 tgctccggaa tgagatagac ggggggcacg gcggtgccgg aggcgatggc catctcctcg
     3181 accacgttga gcaggcgccg ttcggccgga tcggtggtgt tggcgttgat ccggcggcct
     3241 ccgagcgcct ctgcaatggt cttccctccg tcgcgaatga ccaggtactt gtacaggctc
     3301 gctatcccca ccacggcgac cacggctgcg gtgacgatga gccactgctc ggtgggcacg
     3361 ttggtgaagg ccgcggtagc gtccggcgag gtggccactc cgacgtccgt ggtccagcgg
     3421 tagacgccca tcagcaggag gttcgtgagc acgatcagcg agcccacggc ggccgcgaac
     3481 agcagcgcca ggcgccaggt cttgcgcttc gcgtcgtcct gggcgcgaaa gaagttcatc
     3541 cgaccccggg gacgatcagc tgaaggaaac cttcggggcc tcctgcattg cctcgctgtc
     3601 ctcgtactcc agcagggccg cgtcgccagc gtgaccgaag ggcccggcga gcagcatcgc
     3661 aggaaagctc tgcttgtagg tgttgtagtc ggtcacctgg tcgttgtacg cctgccgggc
     3721 gaacgccacg cgattctcgg tgctcgtcag ctcctcggaa acctgcatca tgttctggtt
     3781 cgccttgagg tcggggtagg cctccatcac cacgttcagc cgccccagcg cgccgcccag
     3841 cgccccttcg gcgcttgcca gctgacgaat cgcatcggcg ttgccagggt cctgagcggc
     3901 cgtctgcagg ccggccaggg cctggttgcg ggcggcgatc accgcttcga gggtctcccg
     3961 ctcgtgtgac atgtacgcct tggcggtctc gaccaggttc gggatgaggt cgtagcgccg
     4021 cttgagctgc acctcgatct gcgagaaggc gttcttgaag cggttcttca gggtcaccag
     4081 ccggttgtag atggcgatca cccagaaagc gagcgccgct atgatgacga gcacgatgat
     4141 ggtttccacg atctcctcct tggccggatg tcggtccggg aactccggcg ttcagtccgg
     4201 cagccccagt actcgctttg cgatcacgtt gagctgcacc tcgctggttc ccccctcgat
     4261 ggagttcgcc ttggagcgca gccattgccg cgtggtgccg agctctttgg ctgagaaagc
     4321 ctcgccctcc caacccatgc tctgggtgcc catggcctgc agcatcagct cgtactttcg
     4381 tttgttctgc tcggtgccat agtacttgaa catgctggcc agattgccgt ccgcctgccc
     4441 cgccttggct tcgtccccgc tgcgctccag agtcagctga aaggctcggt cgttcatgcg
     4501 gtgctcgatg acgctgctgc ggacggccgg gtccccgatg cgcccctgtg cctcgccgac
     4561 gtacgccttg gccacgtcct cgagccggcg gccggtgcct ccgagggcgc tcatcccgcc
     4621 gatcccggcc accatctggc gctcgtgctg cagcagccgc ttggcgatgc tccagccctt
     4681 gttgagcccg cccaccag
//
LOCUS       DBNHOX010000589         3713 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1267853, whole genome shotgun sequence.
ACCESSION   DBNHOX010000589 DBNHOX010000000
VERSION     DBNHOX010000589.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1267853"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="AC16UZ_12940"
     CDS             <1..578
                     /locus_tag="AC16UZ_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7TM domain-containing protein"
                     /protein_id="HIR6247634.1"
                     /translation="IMLLPVGALMVVLMRIIVGIRTAGTFMPVLIALAFLQTELLPGI
                     VSFVLVIAIGLVIRSYLSHLNLLLVARIATLVILVIGMISVFSIVSYRLGLIGGLTIT
                     FFPMIILAWTIERMSIIWEEEGPREVFIQGGGSLLVAVLAFLFMDLTAVRHMAFNFPE
                     LHLAVLGLVMLVGRYTGYRLLELRRFIGFKQ"
     gene            575..1561
                     /locus_tag="AC16UZ_12945"
     CDS             575..1561
                     /locus_tag="AC16UZ_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HIR6247635.1"
                     /translation="MIDWITPWELSRRGILGMNRRNIEFIGRYNRREQYPLVDDKLKT
                     KLIAQHAGVQVPDLIDVVRTQHEIETIDDKLADLTEFVIKPAQGSGGKGILVIERHEA
                     GVFTKSSGEQIGILDVKRHLSNVISGLHSLGGRADVAILESLVRVSPLFRNVSYEGVP
                     DIRLIVFQGYPVMGMLRLATLASDGKANLHQGAVGVGLDIGTGKSVAAVQHNRIVRNH
                     PDTGAELTELSVPNWRQLLELAARCFEVTQLGYLGCDIVIDELRGPLLLELNARPGLS
                     IQLANDAGLVPRLRHIENLESFAHNVEDRVDYAMETFTRERRTEQTKLWEGQ"
     gene            1558..1860
                     /locus_tag="AC16UZ_12950"
     CDS             1558..1860
                     /locus_tag="AC16UZ_12950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6247636.1"
                     /translation="MIIVNAIIESDENSIAAMREGITTMVTATLEEPGCDDYCFSVEL
                     DDPTRIRITERWHSIEALAAHFQTPHMAAFQQVMAANPPKNVTAHCYDAEEIDLPR"
     gene            1867..2502
                     /locus_tag="AC16UZ_12955"
     CDS             1867..2502
                     /locus_tag="AC16UZ_12955"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6247637.1"
                     /translation="MLTLWGGATSRTLRAHWMLHELGLDYEPRLIGSRTGATQTDAFK
                     QLNPTEKIPVLVDDEFTLTESVAIVTYLGDTYGSGSGLVPEPFTRDRARYNEWASFIQ
                     MELDAHTLYVLRKHRDLAGIYGEAPAAVATAIGGFAKQVAVADEALRGSAHLIGSKFS
                     AADLLLVSCLDWAAAYGLELTPGLDEYRLRIRERPAYRAAARLNFSISAGA"
     gene            complement(2507..3607)
                     /locus_tag="AC16UZ_12960"
     CDS             complement(2507..3607)
                     /locus_tag="AC16UZ_12960"
                     /inference="COORDINATES: protein motif:HMM:NF014305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247638.1"
                     /translation="MGQDTDVLLEATMALVPPLLTALDALAHAGRHLHPQSLAHLVPQ
                     IDAFARPLRQGARKFEEIPWPPHLERFRDHISASAGEALAAMDAMSACLEQANPVLAA
                     YQAMGAATRATERLYPVSFMLPPVSRFFLTEAGRDDGALARRLERADPGRDDVGVMHA
                     HNGPGERGGFSLYVPEYYDAGEAWPVVVALHGGSGHGRSFLWNWLKDARSRGVLLVAP
                     TSRDRTWSLAGSDADSASLDAIVERVCEHWRVDERRILLTGMSDGGTFSYLSGLRSDT
                     PFTHLSPISASFHPFILEGVDRDRLTGLPIYLVHGTLDWMFPVDVARTARDALTAAGA
                     ALTYREVDDLSHTYPREENARILDWLMGPSAP"
BASE COUNT          662 a         1219 c         1212 g          620 t
ORIGIN      
        1 tgatcatgct gctgccggtg ggcgccctga tggttgtgct catgcggatc atcgtcggca
       61 tccgcacggc gggcaccttc atgccggtgc tgatcgccct ggctttcctg cagacggagc
      121 tgctgccggg catcgtcagc ttcgttctgg tcatcgccat cggcctggtc atccgatcct
      181 acctgtccca tttgaacctg ctgctggtgg cgcgcatcgc cacgctggtg attctggtga
      241 tcggcatgat cagcgtgttc agcatcgtca gttaccgcct cggtctcatc ggcgggctga
      301 ccatcacctt cttccccatg atcatcctgg cctggaccat cgaacggatg tccatcatct
      361 gggaggaaga ggggccgcgg gaggtgttca ttcagggcgg tgggagcctc ctggtggccg
      421 tgctcgcctt cctgttcatg gatctcaccg ccgtccggca catggccttc aatttccccg
      481 aactgcacct ggccgtgctc gggctcgtca tgctggtcgg ccgctacacg ggttaccgcc
      541 tgctcgagct gcgcaggttc atcggtttca agcagtgatt gactggatca caccctggga
      601 gctgtcgcga cgcggcatcc tgggcatgaa ccgacgcaac atcgagttca tcgggcgcta
      661 caaccgccgc gagcagtacc cactggtcga cgacaagctc aagaccaagc tcatcgccca
      721 gcacgcgggc gtccaggtgc ccgatctcat cgacgtggtc cggacccagc acgagatcga
      781 aaccatcgac gacaagctcg cagacctgac ggagttcgtg atcaagccgg cccagggcag
      841 cggcggcaag ggcattctcg tgatagagcg ccacgaggcc ggggtcttca ccaagagcag
      901 cggcgagcag atcggcattc tggacgtcaa acgccacctc tccaacgtca tcagcgggct
      961 gcactccctg ggcgggcgcg ccgatgtggc tattctggaa tctctggtgc gggtcagccc
     1021 gctgttccgg aacgtgagct acgagggtgt tccagacatc cggctgatcg tctttcaggg
     1081 ctatccggtc atgggcatgc tgcgcctggc cactctggcc tccgacggca aggcgaatct
     1141 gcatcagggt gcggtagggg tcgggctcga catcggcacg ggcaagagcg tggccgccgt
     1201 gcagcacaac cggatcgttc gcaatcaccc cgatacaggg gccgagctga ccgagctgag
     1261 cgttccgaac tggcgccagc ttctggagct ggctgcccga tgcttcgagg tgacacagct
     1321 gggctaccta ggctgcgaca tcgtcatcga cgagcttcgc gggccgctgc tgctggagct
     1381 gaacgctcgg ccggggctga gcatccagct ggccaacgac gcggggctgg tgccgcgcct
     1441 gcgacacata gagaatctcg agtctttcgc gcacaacgtc gaggatcgcg tcgactacgc
     1501 catggagacc ttcactcgag agcgcagaac ggaacagacg aaactctggg agggacaatg
     1561 atcatcgtca acgccatcat cgagtcggac gaaaacagca tcgccgccat gcgcgaaggc
     1621 atcaccacca tggtgacggc cacgctcgag gagcccggat gcgacgacta ttgcttcagc
     1681 gtggagctcg acgatccgac caggatccga atcaccgagc gctggcacag catcgaagcg
     1741 ctggccgccc attttcagac gccccacatg gcggcgtttc agcaggtgat ggccgccaac
     1801 ccgccgaaga acgtcaccgc ccactgctac gacgccgaag aaatcgacct gccgcgctga
     1861 gccgtcgtgc tgacgctttg ggggggcgcg accagccgca cgctgcgggc gcactggatg
     1921 ctgcacgagc tcgggctcga ctacgagccc cgcctgatcg gctcgcgcac gggcgccacc
     1981 cagacagacg ccttcaagca gctcaacccc acggagaaga tacccgtgct ggtggacgac
     2041 gaattcacgc tcaccgagag cgtggccatc gtcacgtacc tgggcgacac ctacggctcc
     2101 ggcagcggtc tcgtacctga gcctttcacc cgggaccggg cgcgctacaa cgaatgggcc
     2161 agcttcatcc agatggagct cgacgcgcac acgctctacg tcctgcgcaa acaccgggac
     2221 ctggcaggca tctacggcga agcccccgcc gcggtggcta ccgccattgg cgggtttgcc
     2281 aagcaggtgg ccgtggcaga cgaagcgctc agggggtcgg ctcatctgat cggcagcaag
     2341 ttcagcgcag cagacctgct gctggtcagc tgcctggact gggccgccgc ctacgggctc
     2401 gagctcacgc ccggcctcga cgagtaccgg ttgcggatcc gcgagcggcc cgcttaccgg
     2461 gctgcggccc ggctcaactt ctctatatct gccggagcct gagccctcag ggagccgaag
     2521 gtcccatcag ccagtccagg atgcgggcgt tctcctcccg gggataggtg tgggatagat
     2581 cgtcgacctc gcggtaggtg agcgcggctc cagctgcggt caaggcatcg cgcgccgtgc
     2641 gcgccacgtc gacggggaac atccagtcca gcgtcccgtg cacgaggtat atgggcaggc
     2701 cggtcaggcg atcccgatcc acgccttcca gaatgaaggg atgaaagctg gccgagatgg
     2761 gcgagagatg ggtgaacggg gtatccgagc gcaggccgct cagatagctg aaggttccgc
     2821 cgtcggacat gcccgtgagc agtatgcggc gctcgtccac gcgccaatgc tcgcagaccc
     2881 gttcgacgat ggcgtccagg ctcgcgctgt ccgcatcgga gcctgccagc gaccacgttc
     2941 ggtcgcggga ggtgggcgcg accaggagca cgccccgtga gcgggcatcc ttcaaccagt
     3001 tccagagaaa gctgcgcccg tgaccgctgc cgccgtgcag ggcgaccacc acaggccacg
     3061 cctcgccggc gtcgtagtac tccggaacgt agagggaaaa accgccccgc tcaccaggtc
     3121 cgttgtgcgc atgcatcacc cccacgtcgt cccggccagg atccgcccgc tccaggcgtc
     3181 tcgccagcgc gccgtcgtcg cggccggcct cggtcaggaa aaagcggctg accgggggga
     3241 gcatgaagga gaccgggtac aggcgttcgg tagcgcgggt cgcggcaccc atggcctggt
     3301 aggcggcgag caccgggttc gcctgctcta ggcacgcgct catcgcgtcc atggccgcga
     3361 gcgcttcacc cgcagaagcg ctgatgtgat cgcgaaaccg ttccaggtgg ggcggccacg
     3421 ggatctcttc gaacttgcgc gcgccctgcc ggagcggccg ggcgaacgcg tcgatctgag
     3481 gaaccagatg cgccagggac tgaggatgca ggtgcctgcc cgcatgggcg agggcatcca
     3541 gcgcggtgag cagcggtgga accagcgcca tggttgcttc gagcaggacg tcggtgtctt
     3601 ggcccatgag cgcactatac cctgccgatg gaccagctga gcgccggcgc ccgccgcgtt
     3661 ggatcggcgt cggcagattg attaaagtca tgggttgcgc cactcgaaga tcg
//
LOCUS       DBNHOX010000590         2114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_241007, whole genome shotgun sequence.
ACCESSION   DBNHOX010000590 DBNHOX010000000
VERSION     DBNHOX010000590.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_241007"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2004
                     /locus_tag="AC16UZ_12965"
     CDS             <1..2004
                     /locus_tag="AC16UZ_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR6247639.1"
                     /translation="VNEETTRHANLRGLLTFNKDVNGGPIPIEEVEPATEIVKRFATG
                     AMSFGSISAEAHESLAIAMNRLGGKSNTGEGGEDPSRFIPLENGDSKRSAIKQVASGR
                     FGVTINYLTNADELQIKIIQGAKPGEGGELPGHKVDDIIANIRHSTPGVGLISPPPHH
                     DIYSIEDMAQLIHDLKNANPEARISVKLGAEVGVGTVAAGVTKARADHLVIAGDTGGT
                     GASPLTSIKHAGVPWELGIAETHQTLVVNNLRSRVVLQTDGQLKTGRDVAIAALLGAE
                     EFGFATAPLIALGCIMMRKCHLNTCPVGIATQDPVLRKKFAGQPEHVVNYFFMVAKEL
                     RMIMAELGFSTMNDMVGRVDALNTDDAIDHWKARGLDLSEILTRAEEPAEFLGSYAIH
                     EQDHGLEKALDNELMQLAEPAIKRGEKVYHELGIVNTNRVVGGMLSNKIIREVGPDML
                     PDESIHFKFSGSAGQSFGCWLAKGVTLEVEGDANDYVGKGLSGGRLIIYPPKSSPFKP
                     EEQIIVGNVVLYGATSGECYFRGIAAERFCVRNSGAHAVIEGVGDHGCEYMTGGRVVI
                     LGETGRNFGAGMSGGIAYVWDPQDRFRTQCNMEMVDLERLEDSVEIAEVRGMIERHQQ
                     YTGSTVAEQVLADWEASLGMFAKVMPVDYKRVLEQRRDEVSAG"
BASE COUNT          433 a          673 c          691 g          317 t
ORIGIN      
        1 gtcaacgagg aaaccacccg tcacgccaac ctgcgcggcc tgctcacgtt caacaaggac
       61 gtcaacggtg gccctatccc catcgaggag gtcgagccgg ccacggagat cgtcaagcgg
      121 ttcgccaccg gcgccatgag cttcggctcg atcagcgccg aggcccacga gtcgctggcc
      181 atcgccatga accgcctggg gggcaaatcg aacaccggtg agggcggcga ggaccccagc
      241 cgcttcattc cgctggagaa cggggattcc aagcggagcg ccatcaagca ggtggccagc
      301 ggtcggttcg gcgtgacgat caactatctc accaacgccg acgagctgca gatcaagatc
      361 atccagggcg ccaagccagg cgagggcggc gagctgcccg gccacaaggt ggacgacatc
      421 atcgccaaca tccgccactc gacgcccggc gtcgggctca tcagcccgcc gcctcatcac
      481 gacatctact ccatcgagga catggcccag ctgatccacg acctgaagaa cgcgaacccc
      541 gaggcgcgga tcagcgtcaa gctcggcgcc gaggtgggcg taggaacggt agcggccggc
      601 gtaaccaagg cccgggccga tcatctggtc atcgccggcg acaccggcgg caccggcgcc
      661 tcgccgctga cgtccatcaa gcacgcgggc gtgccctggg aactggggat cgccgagacc
      721 catcagaccc tcgtggtcaa caacctccgt tcgcgggtcg ttctgcagac agacggccag
      781 ctgaaaacgg gccgcgacgt ggccatcgcg gcgcttctcg gcgcagagga gttcggattc
      841 gccaccgcgc cgctcatcgc gctgggctgc atcatgatgc gcaagtgtca tctgaacacc
      901 tgcccggtcg gcatcgccac ccaggatcca gttcttcgca agaagttcgc cgggcaaccg
      961 gaacacgtgg tgaactactt cttcatggtg gccaaagagc tgcgcatgat catggccgag
     1021 ctcggcttct caacgatgaa cgacatggtc ggccgggtgg acgcgctgaa caccgatgac
     1081 gccatcgatc actggaaggc caggggtctg gacctcagcg agattctcac gcgagccgag
     1141 gagccggccg agttcctggg ctcctacgcg atccacgagc aggaccacgg gctggaaaag
     1201 gcgctggaca acgagctcat gcagctcgcc gagccggcca tcaagcgcgg cgagaaggtc
     1261 tatcacgagc tcggcatcgt caacaccaac cgcgtggtgg gcggcatgct gtcgaacaag
     1321 atcatccggg aagtgggtcc ggacatgctt ccggacgaat ccatccactt caagttttcg
     1381 ggaagcgccg gccagagctt cggctgctgg ctggccaaag gcgtgacgct ggaggtagag
     1441 ggagacgcca acgactatgt cggcaaaggc ctctccggcg gccgcctcat catctacccg
     1501 ccgaagtcga gcccgttcaa gccggaagag cagatcatcg tcggcaacgt cgtgctctac
     1561 ggagccacca gcggcgagtg ctacttccgc ggcatcgccg cggagcgttt ctgcgtgcgt
     1621 aacagcgggg cgcacgcagt gatcgagggc gtcggcgacc acggctgcga gtacatgacc
     1681 ggcgggcgcg tcgtcatcct gggcgagacg ggacgcaact tcggcgccgg catgagcggc
     1741 ggcattgcct acgtctggga tccgcaagac aggttccgga cccagtgcaa catggagatg
     1801 gtggatctgg aacgtctaga ggactccgtt gagatcgccg aagtgcgcgg catgatcgag
     1861 cggcaccagc agtacacggg ctcgacggtt gccgagcagg tgctcgcgga ctgggaggct
     1921 tcgctgggga tgttcgccaa ggtgatgccc gtcgactaca aacgggtgct ggaacagcgc
     1981 cgcgacgaag tgagcgcggg ttgaacatgg gtaaagttac cggattcatg gagttcgatc
     2041 gcaacgcggc gccgtaccgg aacgccggca aacgtctgct cgatttccag gagatctaca
     2101 ccgatcacga cgag
//
LOCUS       DBNHOX010000591         3051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_499255, whole genome shotgun sequence.
ACCESSION   DBNHOX010000591 DBNHOX010000000
VERSION     DBNHOX010000591.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_499255"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..1321)
                     /locus_tag="AC16UZ_12970"
     CDS             complement(251..1321)
                     /locus_tag="AC16UZ_12970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HIR6247640.1"
                     /translation="MRYRTLHEIVRQAQRNLDRDCRDYLLGGADTESSLRRNRHGLDS
                     WVFRPRILNDVSEVNLTIELLGAPMRIPVVLPPIGSVQLFEPGGGQTVAEAAHEFGIL
                     QILSSVCTPDFEAVAAAVPGPRIYQLYLMGDRVWLDDMIGRTIEAGYTGFCLTADTQI
                     YSRRERDILKGFVPPSGSQAGSRDFNYQARMTWETVAHIKSTFDIPLIVKGVNAGEDA
                     ARCVEAGVDVVYVSNHGGRQLDHTRACIDALPEVVEAVAGRVPVIVDGGFLRGADVVK
                     GLCLGADVVGMGRLEGLAMAAGGRGALVRALEIVEQEIRTTMALLGVSDLADLNPGLM
                     ERAQPLAESTVFSAFPLLEEGY"
     gene            1493..2698
                     /locus_tag="AC16UZ_12975"
     CDS             1493..2698
                     /locus_tag="AC16UZ_12975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6247641.1"
                     /translation="MTGMVIVGAGHAAGQAAASLRQEGYEGEITIIGDEPHIPYQRPP
                     LSKQYLAGEQGLERVYLRPEKFYADKEITLRMGVSVTAIDTQAHTVTTDDGETIGYEK
                     LLLATGSRPRILNVPGSDLAGIHYLRTIADVDGIRAELAEGRRLVIVGGGYIGLEVAS
                     VGIQAGLEVHVLEMEERILQRVTTPEMSDYYHQLHTGRGVHIHTSTMVSGFTGEGRVG
                     AVQCGDEEIAADIVVVGIGIIPNVEVAQAAGIECDNGIVVDEGCRTSADDVFAAGDCT
                     NHPNPLLHRRLRLESVPNAMEQARVAAANMCGADKVYESIPWFWSDQYELKLQMVGFS
                     ADGDTQVVRGDKAANQFAVFYLKDGAVVAADAVNSPKEFMVCKQLVGKPVDAARLGDP
                     DVELKSLLA"
BASE COUNT          572 a         1013 c          974 g          492 t
ORIGIN      
        1 cgacgacagg ctcatcccgg acaggctggc atccgtgccc ggcgggcgtg gcgcatcgtc
       61 cccgcctcct ccgccaccgc atgccgccat ggtgaagacg atgagaaacg ttccaaacca
      121 gcggatagac gttcgcaatg taggtgccat gaccggacct tccgcaaata aactgcccaa
      181 gtggcgacgg tgtaaccgta acatatcttg acgtatttga aactaggtca cttcccttgg
      241 ttgcaggggc tcaatacccc tcttccagca gcgggaacgc gctgaaaacc gtggactccg
      301 ccagaggctg agcccgctcc atgaggcccg gattgagatc ggcaagatcg gagacgccca
      361 gaagcgccat ggtggtccgg atttcctgct ccacgatctc cagcgcccga acgagggccc
      421 cacgtcctcc ggccgccatg gcgaggccct cgagacgacc catcccgacc acgtcggccc
      481 ccaggcacag gcccttgacc acatcggcac cacgcagaaa accgccgtcg acgattaccg
      541 gcacgcggcc ggccacggcc tcgacgacct ccggcagcgc gtcgatgcag gccctggtgt
      601 ggtccagctg acgaccaccg tggttcgaaa cgtaaacgac gtccacaccc gcctccacgc
      661 agcgcgccgc gtcctcgccc gcgttgacgc ccttgacgat gagcggtatg tcgaacgtgc
      721 tcttgatgtg cgccaccgtc tcccaggtca ttcgcgcctg gtagttgaag tcccggctgc
      781 cggcctggct gcccgacggg ggcacgaagc ccttgagaat gtcccgctcg cgcctggagt
      841 agatctgggt gtcggccgtc aggcagaagc ccgtgtaccc ggcttcgatg gtccggccga
      901 tcatgtcgtc cagccagacc cgatcgccca tcaggtagag ctggtagatc ctgggtccag
      961 gcaccgcggc ggccacggcc tcgaaatcgg gcgtgcacac ggagctcagg atctggagga
     1021 tgccgaactc gtgcgccgcc tcggcaaccg tctggccgcc ccccggctcg aacagctgca
     1081 ccgaaccgat gggcggaaga accaccggga tgcgcatcgg cgcgcccagc agctcgatgg
     1141 tcagattcac ctcgctgacg tcgttcagga tacgcggacg aaagacccag gagtcgagcc
     1201 catggcggtt gcgccgcagc gaagactcgg tgtccgcgcc gccgaggagg tagtcccggc
     1261 agtcgcgatc cagattgcgc tgggcctgcc tcacgatctc atgcagcgtc cgatacctca
     1321 tcgcgatttc caccttcgat gctcgaccgc ggatgctaac gcctgcgccg gacaaatcca
     1381 tcgacaatcc cggtttcggc tcgtcctgag caagggaacc ttagtaagat gcccctccag
     1441 cgcgggccgg cgcacgggcc ggggcgagcg gacgaaccaa ggagctgagc aaatgacggg
     1501 gatggttatc gtgggggccg gacatgctgc ggggcaggcg gctgccagcc tgcgccagga
     1561 gggttacgaa ggtgagatca ccatcatcgg ggacgagccg cacataccct accagcggcc
     1621 acccctgtcc aagcagtacc tggccggaga gcagggcctg gaaagggtgt acctgcgccc
     1681 cgaaaagttc tacgcggaca aggaaattac cctgcgcatg ggcgtcagcg tcaccgcgat
     1741 agacacccag gcacacaccg tgaccacgga cgacggcgaa accatcggct acgagaagct
     1801 cctgctcgcc acgggcagtc ggcccaggat tctcaacgta cccggcagcg accttgcggg
     1861 cattcactac ctgcggacca ttgccgacgt ggacggcatt cgcgcggagc tggctgaagg
     1921 gcgccggctc gtcatcgtcg ggggcggcta tatcgggctc gaggtggcgt cagtgggaat
     1981 ccaggcgggc ctggaggtcc acgtgctgga gatggaagag cgcatcctgc agagggtcac
     2041 aacgcccgag atgtccgact attaccacca gctgcacacc ggccgggggg tgcacatcca
     2101 tacctccacc atggtgagcg gcttcaccgg cgagggccgc gtcggcgcag tgcagtgcgg
     2161 cgacgaggag attgccgccg acatcgtggt cgtgggcatc ggcatcatcc ccaacgtcga
     2221 ggttgcccag gccgccggga tcgagtgcga caacggcatc gtcgtggacg aaggctgccg
     2281 cacgtcggcg gacgacgtgt tcgccgccgg cgactgcacc aatcatccca acccgctgct
     2341 ccaccggcgc ctgcgcctgg aatccgtgcc caatgccatg gagcaggccc gggtagcggc
     2401 cgcgaacatg tgcggcgcag acaaggtgta cgagtcgatt ccatggttct ggtcggacca
     2461 gtacgagctg aaactccaga tggtcggctt ctccgccgac ggcgacacgc aggtggtccg
     2521 gggcgacaag gcagccaacc agtttgccgt gttctacctc aaagacggcg cggtggtggc
     2581 cgccgacgcg gtgaacagcc cgaaagagtt catggtgtgc aagcagctcg tgggcaagcc
     2641 ggtggatgcg gccaggctgg gcgatccgga tgtggagctg aagtccctgc tggcttgagc
     2701 agcctcaaag gcggccgatc gacaccttcg ccgtccgtgc catacgtaga atccctgatg
     2761 ggtatgggca cagggaaaga tgacaatgat ttctgcgaca ggtgcgcggg atcgtctcct
     2821 cctctcccct tcccgcgcgt cttttcacgc cggatcctct ggatccggcg tttctttatg
     2881 ggcccacttc gcccggggcc gctcagtcca cggacaggga aaggacccgg gaaatctggc
     2941 acagctcgcg cccgctctcc gcgtgccatt cgttgaaggc gtcctggacc cgcgcgagcg
     3001 ctttcttgct ggtgggctcc cgatcgatca cgccctgacc tatcagggct t
//
LOCUS       DBNHOX010000592         2548 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_305379, whole genome shotgun sequence.
ACCESSION   DBNHOX010000592 DBNHOX010000000
VERSION     DBNHOX010000592.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_305379"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /gene="gatB"
                     /locus_tag="AC16UZ_12980"
     CDS             <1..189
                     /gene="gatB"
                     /locus_tag="AC16UZ_12980"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792781.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR6247642.1"
                     /translation="GEIEAIIAGIVAENPDQVAQFRAGKEKVLGFFVGQVMKATQGKA
                     NPKQVNELLRKALAADTG"
     gene            complement(186..626)
                     /locus_tag="AC16UZ_12985"
     CDS             complement(186..626)
                     /locus_tag="AC16UZ_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6247643.1"
                     /translation="MKSVKVADYMSTRLITFNRTDSVVEAMAVLLKKKISGAPVVDDE
                     GNLIGILSEKDLMAVVIQDSYYDEGVGIVGDYMREEVDTVDPDSDIYSLADRFHKEGR
                     RRFPVVKNGRLVGQISRRDVLRAAEEFLMHADGLSERKRRKLLT"
     gene            769..1185
                     /locus_tag="AC16UZ_12990"
     CDS             769..1185
                     /locus_tag="AC16UZ_12990"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6247644.1"
                     /translation="MSDTPLPVVEYLKIPEDGDPYLEGYKCGSCGATFLGERSVCSKC
                     GARDQMSAVTLPNHGTLYSYSIVHRSFPGIDVPYVSAVVDLDDGTAIKGNLINVEPDP
                     DKIEFGMPVDVVFADALGRKDRDGNSYLSFFFQPSS"
     gene            1210..2355
                     /locus_tag="AC16UZ_12995"
     CDS             1210..2355
                     /locus_tag="AC16UZ_12995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6247645.1"
                     /translation="MTDAYVVGVDMIKFGRFPEKTVPEIGAESALMALDDCGLTIQDM
                     EALYCGNLGQASGMVGQRILQQIGQTGIPVVNVSNACATGATAFREAWASVKAGLYDC
                     VLAVGVEQMGKGLLGGAGGGTGIPKEGLLGSGTMPAVFAEAGMEHTRKYGTTFEQFAK
                     VSVKNHHHSTMNPKAMYQIETPLETVMNAEMISYPNTKLMCSVNVDGSAAAVICSEAK
                     AKELGLMKRAVKVRASVLTSDPWQERDLVMPDVNSCTRKAAAAAYEMAGLGPDDIDLI
                     ELHDCFATAEILHYENLGLCGDGEAGRLIDDGEVALGGRIPVNVSGGLLSKGHPLGAT
                     GIANIYEVCTHLRGEAGQRQVEGARIGLTHVIGLGSACGIHIHEKTA"
     gene            2446..>2548
                     /locus_tag="AC16UZ_13000"
     CDS             2446..>2548
                     /locus_tag="AC16UZ_13000"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6247646.1"
                     /translation="MKIGVVSDTHNNLRNVGRIVELFNEYGVERVIHT"
BASE COUNT          480 a          850 c          792 g          426 t
ORIGIN      
        1 ggcgagatcg aggccatcat cgccgggatc gtggcggaga acccggatca ggtcgcccag
       61 ttccgcgcgg gcaaagagaa ggtgctgggt ttcttcgtcg ggcaggtcat gaaggcgacc
      121 cagggcaagg cgaaccccaa gcaggtgaac gagctcctcc gcaaggccct ggcagccgac
      181 accggctagg tcagaagctt ccttcgcttc cgctcgctca gcccgtcggc atgcatgagg
      241 aattcctccg ccgcccgcag cacgtcccgg cggctgatct ggccgaccag gcggccgttc
      301 ttgaccacgg ggaagcgccg cctgccctct ttgtgaaacc ggtccgcaag gctgtagatg
      361 tccgaatccg ggtcgacggt atccacctcc tcgcgcatgt agtcgcccac gatgcccacg
      421 ccctcgtcgt agtagctgtc ctggatgacc accgccatca ggtctttctc agacaggata
      481 ccgatgaggt tgccctcgtc gtccaccacc ggcgcgccgg aaatcttctt cttcagcagg
      541 acggccatgg cctccaccac gctgtcggtg cggttgaacg tgatgagacg cgtggacatg
      601 taatccgcga ctttgactga cttcaccggc tctccccagt tgatccgctg gccgctgaaa
      661 cggctcttca gtacattgct gcaaaacccc ctcccacgcc aggtgacgcc agggggccgt
      721 caatagtatc ctcccggggc tgggccacaa gagtttatgg gggacacttt gagcgacaca
      781 ccactgcccg tcgtggaata tctgaagatt ccggaggacg gcgatcccta cctggagggt
      841 tacaagtgcg gcagctgcgg cgccacgttc ttgggcgagc gcagcgtgtg ctccaagtgc
      901 ggggcccgtg accagatgtc ggccgtgacg ctgccgaacc acggcaccct gtattcctac
      961 tccatcgtcc accgctcgtt cccgggcatc gacgtgccct acgtgagcgc ggtggtcgac
     1021 ttagacgacg ggacagcgat caaaggcaac ctgatcaacg tcgagcccga cccggacaag
     1081 atcgagttcg gcatgccggt ggacgtcgtc ttcgccgacg cgctagggcg caaggacagg
     1141 gacggcaact cgtacctgtc tttctttttt caacccagtt cctgacccga ttcgaatagg
     1201 aggatctcca tgacagacgc atacgtagtc ggtgtcgaca tgatcaagtt cggccgcttc
     1261 cccgagaaga cggtcccaga gatcggcgcc gaatccgcgc tgatggcctt agacgattgc
     1321 gggctcacca tccaggacat ggaggcgctt tactgcggca acctgggtca ggccagcggc
     1381 atggtcggcc agcgcatcct gcagcagatc ggccagacgg gcatacccgt cgtcaacgtc
     1441 tccaacgcct gcgccaccgg cgccactgcc ttcagggaag cctgggcatc cgtcaaggcc
     1501 gggctttacg actgcgttct cgccgtaggc gtcgagcaga tgggcaaggg tctgctgggc
     1561 ggggccggcg gcggcaccgg cattcccaag gagggcctct tgggctccgg caccatgccg
     1621 gccgtgttcg cggaagccgg catggagcac acgcgcaagt acggcaccac cttcgagcag
     1681 ttcgccaagg tgtcggtgaa gaaccatcac cactccacca tgaacccgaa ggccatgtac
     1741 cagatcgaga cgcccctgga gacggtgatg aacgccgaga tgatctccta ccccaacacc
     1801 aagctgatgt gctcggtgaa cgtggacggc tccgccgctg cggtgatctg ctccgaggcg
     1861 aaggccaagg agctgggtct gatgaagcgg gccgtgaagg ttcgcgcctc cgtgctcacc
     1921 tcggatccat ggcaggagcg cgacctggtc atgccggacg tcaactcctg cacccgcaag
     1981 gcggccgcgg cagcctacga gatggccggg ctcggcccgg acgacatcga tctcatcgag
     2041 ctccacgact gcttcgccac cgccgagatc ctccactacg agaatctggg tctttgcggt
     2101 gacggtgaag cgggccggct gatcgacgac ggcgaggtgg cgctcggcgg ccgcatcccg
     2161 gtgaacgtct ccggagggct gctctccaag ggccaccccc tgggcgcgac cgggatcgcc
     2221 aacatctacg aggtctgcac ccacctgcgt ggcgaggccg gacaacggca ggtcgagggc
     2281 gcgcgcatcg ggcttaccca cgtgatcggg ctgggcagcg cctgcggcat tcacatccac
     2341 gagaagacgg cctaaaccac agccatcgcc gtcccaagtg gccgccccgg ccgtcaggcc
     2401 ggggccgcac tgaaggccgg cccttcgcct ccaaccacca agcccatgaa aatcggcgtc
     2461 gtcagcgaca cccacaacaa cctgcgcaac gtgggtcgaa tcgtggagct cttcaacgag
     2521 tacggcgtcg agcgcgtgat ccacaccg
//
LOCUS       DBNHOX010000593         1672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1719420, whole genome shotgun sequence.
ACCESSION   DBNHOX010000593 DBNHOX010000000
VERSION     DBNHOX010000593.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1719420"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="AC16UZ_13005"
     CDS             <1..485
                     /locus_tag="AC16UZ_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6247647.1"
                     /translation="LPQGCYYQAALGWPHMFMTLCPTYLGIAEAAFDFTCQYLRGEVE
                     GGPPAGGARDNPVKQVGVAEMRIKLEQARALFEKAIGEAAYRPSKDERLRAYAAQYTV
                     MEFANDICRQAIRVCGGRSLFKSLRLEQLYRDSRCGSLMLPWTPDICIERLGRESLES
                     "
     gene            490..>1672
                     /locus_tag="AC16UZ_13010"
     CDS             490..>1672
                     /locus_tag="AC16UZ_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6247648.1"
                     /translation="MNGLGDWIRCNADWAGGRPALRLGETAYTYAELDAAVDRMAFLL
                     RDGLGIRRGDVVAFLGLNSEQMIVLLFACARNAASLLPLNNRLAGPEHEWILRHSGAR
                     FLFVEDEFAAKANAIDVSGLERVAMGAPPEPHWHEFQRMLGDAREGGFEDVGTLDDRV
                     LLIYTSGTTGQPKGVAHDQRALFYNALNAIHAQEMRASDHVLTVLPLFHAGGLNIQTT
                     PAFYIGAGVSLMRRFDPGEALRAIETLKPTMFLAVPAVITAMVDHPAWSETDLSSLRM
                     VGTGSSTVPDPLLDAWHDRGVPATQIYGLTESAPTAICLPIAETRRKRGAAGKPVIHC
                     EARIVDDGGNILGPGERGEIVLKGPNLFREYLNDPETTAAAFSDGWFHTGDIGHMDAE
                     GF"
BASE COUNT          270 a          554 c          563 g          285 t
ORIGIN      
        1 ttctgcccca gggctgctac taccaggcgg cgctgggctg gccgcacatg ttcatgaccc
       61 tctgccccac ctacctcggc atcgctgaag ccgccttcga tttcacctgc cagtacctgc
      121 gcggggaggt ggaaggcggc ccgccggcgg gcggcgcccg cgacaacccg gtcaagcagg
      181 tgggcgtcgc cgagatgcgc atcaagctgg agcaggcccg ggcgctcttc gagaaagcca
      241 tcggggaggc cgcctaccga ccgagcaaag acgagcggct gcgcgcttac gccgctcagt
      301 acacggtcat ggagtttgcc aacgacatct gccggcaggc cattcgcgtg tgcggcgggc
      361 gctcgctgtt caaatccctg cggctggagc agctttaccg ggattcccgc tgcggctcgc
      421 tcatgctccc ctggacgccc gacatctgca tcgaacgcct cgggcgggaa agtctggaga
      481 gctgaggcgt tgaacggtct cggcgactgg atccgctgca acgctgactg ggcaggcggc
      541 cgcccggcgc tccgtctggg cgagactgca tacacctacg cggagctcga cgccgccgtg
      601 gatcgcatgg cctttctgct gcgcgacggc ctgggcatcc ggcgcggcga cgtggtggcg
      661 tttctcggcc tcaacagcga gcagatgatc gttttgctgt tcgcgtgcgc ccgcaacgcg
      721 gccagcctgc tccccctgaa caatcgcctg gccggcccgg aacacgagtg gatactgcgt
      781 cacagcggcg cgcgctttct cttcgttgaa gacgagtttg ctgccaaggc gaacgcgatc
      841 gatgtgtcgg gcctggagcg ggtggccatg ggtgcgccgc cggagccaca ttggcacgag
      901 tttcagcgca tgctcggtga cgcaagggaa ggcggtttcg aagacgtggg aacgctggat
      961 gatcgggtgc tgctgattta cacctccggc accaccggcc agccgaaggg cgtggcgcac
     1021 gaccagcgtg cgcttttcta caacgcgctg aacgcgattc acgctcagga gatgcgggcg
     1081 tcggaccacg tgctcaccgt cctgccgctc ttccatgcgg gcggtctcaa cattcagacc
     1141 acgccggctt tttacattgg ggccggcgtg agcctgatgc ggcgcttcga tccgggtgag
     1201 gccctcaggg ccatcgagac gctcaagccc accatgttcc tggcggtacc ggcggtcatc
     1261 accgccatgg tcgaccaccc ggcgtggtcg gagaccgacc tctcctccct gcgtatggtg
     1321 ggcaccggct cgtctaccgt accggacccg ctgctcgacg cttggcacga ccgcggggtg
     1381 cccgccacgc agatatacgg gctcacggag agcgcgccga cggccatctg cctgcccatc
     1441 gccgagaccc gacgcaagcg gggggcggcg ggcaaaccgg tcattcactg cgaggcgcgg
     1501 atcgtggacg atgggggaaa catcctcggg ccgggggagc gtggcgagat cgttctgaaa
     1561 ggcccgaatc ttttccgcga gtatctgaat gacccggaga ccacggccgc cgcgttctcc
     1621 gacggctggt ttcataccgg ggacatcggc cacatggacg ccgaagggtt ct
//
LOCUS       DBNHOX010000594         9124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1204873, whole genome shotgun sequence.
ACCESSION   DBNHOX010000594 DBNHOX010000000
VERSION     DBNHOX010000594.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9124
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1204873"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="AC16UZ_13015"
     CDS             <1..1542
                     /locus_tag="AC16UZ_13015"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR6247649.1"
                     /translation="LAGPVPDRRGRSRILSGACWCLLILCVSGCVYLAQPEDAVVSQA
                     QAPDPQSRIGQLLGGGRGVPARGLLVEASDLALTARIDMIDAADATIDAQYFIWQNDP
                     TGVLVIQKLLDAADRGVRVRALLDDVQLTGFVDRLNALDDHPNIEIRIFNPFSVRFRY
                     PLGFIRIAEFAIDGARLNHRMHNKLMVADNQLAILGGRNIGDDYFGRNPKRNFVDTDI
                     LLSGDIVPELSRGFDSYWNSRWAYPVSALMEFSLLPVDLETIRQRIRDRLEEWPDLES
                     LGREIAFEDTLADLRDAAALDWSTTVVDDPEVGWFERPDDIALDLTELALRAEREVLV
                     VTPYLIPTPNAFKIGKELVDKGVRVRVVTNSLGTNDVVAAQAAYGRYRKRILDTGVEL
                     YELRADAALPERGLSEEVCLHSKYIIIDDEIVFVGSLNLDPRSLYLNTEVGVVLESRP
                     LAATLRESFDLLAHPDSSWRVTTHENGFRWESSAGTLDRQPAKNGWQRFRYWFLSLIP
                     VSSQL"
     gene            1678..2796
                     /locus_tag="AC16UZ_13020"
     CDS             1678..2796
                     /locus_tag="AC16UZ_13020"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6247650.1"
                     /translation="MSYPIISADSHITEHPDTYSAHIDPAWRDRAPKMIDGGERGDLF
                     VIDGMDTPIAMGLVAAAGKPAEELTMAGVRFEELHRSGWDPAARMADQDRDGVAAEII
                     YPTVGMLLCNHRDFDYKKACFDAYNRWIAEYCSTNPARLLGCGQTAVRSPAETVSDLE
                     KIRELGLRGVMMPGDPLEEDYDSQIYNPVWEAAIDLGLPLSFHILTTRQQAPVRGPRI
                     NSFLSIIRGNQDIMGTLIFGGVFDRYPELKVVCVEADAGWVPHYMYRMDHAYKRHRNW
                     LAKGVSLARLPSEYFSEHVYTTFQDDWVAFRLVNDMNWRRLLWANDFPHSDSTWPWSQ
                     QMLAEQTANLTEEQKRGILCDNVAELYRIDVDALAQAA"
     gene            2796..3119
                     /locus_tag="AC16UZ_13025"
     CDS             2796..3119
                     /locus_tag="AC16UZ_13025"
                     /inference="COORDINATES: protein motif:HMM:NF049702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7508 domain-containing protein"
                     /protein_id="HIR6247651.1"
                     /translation="MSIRLTKPWSPASAAGKLPGQMGVYQLGDEAGNVITIGYAGGRS
                     TFGLRGEVETALQRHPDAVSYRVEVNSAYLSRYRELLMVHLADFGTLPPGNEALPGLG
                     RLSPA"
     gene            3129..4295
                     /locus_tag="AC16UZ_13030"
     CDS             3129..4295
                     /locus_tag="AC16UZ_13030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6247652.1"
                     /translation="MDLDLSEEQNLLIGTVRRFVREEILPLEQSLDPDADELEPDDHE
                     RLTQLTCEMGLYGLDVPPEFGGPDVDLVTRTLLAMEMSQHRAGLYAPCYGVFGGAGLA
                     QLFEATEDQKDRYLFPLLRGEKKGFFGLTEPSGGSDPARAIQTRAVRDGDEWVVNGSK
                     IFISGADRADFGLVFARTDADKGRNGVTCFIVDTDTPGFHVRRIVHTLRTARYATELS
                     FEDMRVAHGNVLGEVNGGFAIANDRLSRQRIPYAAGCIGVAIKAHEMALEYVPQRETF
                     GAPLSTRQAIQWMLVDNEIDIRQASWLTLEAAHKADKGEPFRKEAAMAKLVATEAGSR
                     VVDRCMQMFGGYGVTKDFPFERWYREMRIRRIGEGPNEVQRHIIARDILGASLR"
     gene            4367..5410
                     /locus_tag="AC16UZ_13035"
     CDS             4367..5410
                     /locus_tag="AC16UZ_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247653.1"
                     /translation="MLQGRYSEAELYSEHDYARPHQIAGRRLHGGFDAAGNYVPPRSK
                     GRSEAIEAWTRALRERGGELFDADASLLSGPRMPTVEQQHMLLREGIGRPFWNTLTVT
                     GKIEGRGRVLAEMQFPDLQDVVVEDISDKAVGHLNRGLLAIHGIDEGGQPELGLGGHD
                     VMWFVARDLVFGPGAYPDVEPPESIARPEAGKRWMPEISAEHEALLSFLMNLLIIEFR
                     AEIGFAATQDILRADDLFTDRRTRACEAAEIIERIRADEEIHVCSLRLYLGELRQLTL
                     RTVDGGTVPAAELIDGFWDGLVRWATVEQPRLAAERQYAAIRTEILEHPDGERVLAAF
                     DALSDPAGLASAG"
     gene            complement(5426..6214)
                     /locus_tag="AC16UZ_13040"
     CDS             complement(5426..6214)
                     /locus_tag="AC16UZ_13040"
                     /inference="COORDINATES: protein motif:HMM:NF014635.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HIR6247654.1"
                     /translation="MSDLITTDQTSYDWAELVAGLNRLLRLRTTPIGMKLFETRGEME
                     AVPRIRRPRDIHTTDQIVGQAARNGWTVGITAEDLVGAQCSTVIGLHPRSDDWLSGDR
                     MTGVWYETGEDASAHQHAMDVVPHGRYEAMAVSPLASGRLDPPDICLIYATPAQMILL
                     INGLQWSGYRKLEWGCVGESACADSWGRALATGEPSLSIPCFAERRYGGVMEDELLMA
                     MPPKFLPKTLAGLEQLSRNGLRYPIPQYGVNNDVRAGMGVSYGR"
     gene            complement(6246..7079)
                     /locus_tag="AC16UZ_13045"
     CDS             complement(6246..7079)
                     /locus_tag="AC16UZ_13045"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6247655.1"
                     /translation="MYEEITYTVEDPVAIITMNRPDRLNAFTVRMLAEIRHALGAAEK
                     DGRVVGIVLTGAGRGFCAGMDMGSLDRMSSGGEAAAEDLSGLEAEVGDPGMGEDFQVT
                     YGYLMAVQKPVIAAVGGACAGLGFVIAMLTDMRFVEPQAKFTTAFSQRGLVAEHGVSW
                     ILPRLIGPGRALDILWSSRKFDGTEAERIGIAERLVDEGAAVSEACDYIRDLAANASP
                     TSLKMMKAQVYRHLNMSLGEAMRESNAWMAQSLKREDFTEGVRSFIERRPPRFKRVGE
                     D"
     gene            7222..7959
                     /locus_tag="AC16UZ_13050"
     CDS             7222..7959
                     /locus_tag="AC16UZ_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247656.1"
                     /translation="MSRSLSWFLAFLVLLGVLAVAGSWYLGPSLVGLAFDADRREAPY
                     YVLSLAGRSELADADLRFGSDLAELFDAAGGKLLWRGTTERVVKGRLQDEWLNAQLFA
                     FPRGANFVELKTGGDYRDLLAAYEGRSRLLLGSPTQPDRMARGRIWVLNLIAEDAEDL
                     QATGGDPLAGLMGGVAGFGGSLVWDAKVADLDERSSWNRLVLLGFAQPLEAEVWLSDA
                     GTQTELALSMERARRRVTLLMRPADPI"
     gene            complement(7956..8642)
                     /locus_tag="AC16UZ_13055"
     CDS             complement(7956..8642)
                     /locus_tag="AC16UZ_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR6247657.1"
                     /translation="MTNTGPATARPTDMGDAQQRLNATLPMARLEPVTLPDCSGMRLA
                     LINADFPTGPLPGEVMSAVVERPAYWAFCWGSGLGLAQFLLRNPEWVAGKSVVDLGSG
                     SGVAGIAAARAGAARVVACDTDADARLATSVNAAMNGVRLEVAATLPARCDLMLMADV
                     LYDRANLPLLSTAQRHADEVLVADSRIRELPDPEYVQVTTIDAATFPNLGEFDEFGTV
                     RVFHRPSARV"
     gene            8932..>9124
                     /locus_tag="AC16UZ_13060"
     CDS             8932..>9124
                     /locus_tag="AC16UZ_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247658.1"
                     /translation="MQSRLCIACNWLVMLGALAIADLAAAEAAEAAGCDAAEYRAFDF
                     WVGDWEVRLADGSLAGHNAV"
BASE COUNT         1462 a         2938 c         3115 g         1609 t
ORIGIN      
        1 ctggccggac ccgtgcctga tcgtcgcggc cgctcgagaa ttctctccgg cgcctgctgg
       61 tgcctgctca tcctctgcgt atccgggtgc gtctacctgg cgcagccgga ggatgccgtg
      121 gtcagtcagg cccaggcgcc cgatccgcaa tcccggatcg gtcagcttct cggcggcggg
      181 cgcggggtgc ccgctcgggg actgctggta gaggcctccg acctggcgct gaccgcccgc
      241 atcgacatga tcgacgcggc ggacgcgacc atcgatgcgc agtacttcat ctggcagaac
      301 gacccgacgg gcgttctggt aatccagaag ctgctggacg cggcggaccg gggggttcgg
      361 gtacgcgcgc tgctagacga cgtgcagctc accgggttcg tcgaccggct gaacgcgctg
      421 gacgaccatc cgaacatcga gatccggatc ttcaatccct tcagcgtgcg ctttcgctac
      481 ccgctgggtt tcatccgcat cgccgagttc gccatcgacg gcgcgcggct gaatcatcgg
      541 atgcacaaca agctcatggt cgccgacaac cagctggcca tcctcggcgg tcgcaacatc
      601 ggcgacgact attttggcag gaacccgaag cgcaacttcg tcgacacgga catcctgttg
      661 tccggcgaca tcgttccgga gctgtcccgg ggattcgatt cctactggaa cagccgctgg
      721 gcttatcccg tgagcgcgct gatggagttc tccctgctgc cggtggatct ggagacgata
      781 cgtcagcgga tccgtgaccg cctcgaggag tggcccgatc tggaatcgct cggccgcgag
      841 atcgcgttcg aggacaccct tgccgatctg cgcgacgctg cggcgctgga ctggtcgacc
      901 acggtggtcg acgaccccga ggtcggctgg ttcgaacgcc cggacgacat tgccctggat
      961 cttacggagc tcgcgctgcg tgccgagcgc gaggtgctgg tggttactcc ttacctgatc
     1021 cccacgccca acgccttcaa gatcggaaag gagctcgtcg acaagggggt gagggtgagg
     1081 gtcgtcacca actccctggg aaccaacgac gtggtcgcgg cccaggcggc ctacgggcgc
     1141 taccgcaaac gcatcctgga tacgggcgtc gagctttacg agctgcgggc agacgcggcg
     1201 ttgccggagc gtgggctctc cgaggaggtc tgcctgcact ccaagtacat catcatcgat
     1261 gacgagatcg tcttcgtggg ctcgctgaac ttggatccca ggtccctcta cctgaacacg
     1321 gaggtcgggg tggtgctgga gtcgcgacct ctggcggcga cgctgcgaga gtccttcgat
     1381 ctgctcgccc atcccgacag ctcatggcgg gtcacgaccc acgagaacgg attccgctgg
     1441 gagtcgtcgg ccggaacgct cgatcgccag cctgcaaaga acggctggca gcgtttccgt
     1501 tactggttct tgagcctcat cccggtctcc agccagctct gaggcctggg gttcggggct
     1561 ttcgccgcgc gcggcgaagg ggcgcgggcg gcccgctgat ccgtccgcgg cgccttgaca
     1621 ggcagcgccg tcggccggga tagtcggtgg cgtgatcgtc atcaaagggg agagacgatg
     1681 agctatccga tcatctcggc cgattcccac atcaccgagc atccggacac ctactcggcg
     1741 cacatcgatc cggcctggcg ggatcgcgct ccgaaaatga tcgacggcgg tgagcggggc
     1801 gacctcttcg tcatcgacgg gatggacacc ccgattgcca tggggctggt ggctgcggct
     1861 ggaaagcctg cggaggaact caccatggcg ggtgtgcgct tcgaggagct gcaccgcagc
     1921 ggctgggatc cggcagcccg catggcggat caggaccggg acggggtagc cgcggagatc
     1981 atctacccga ccgttggcat gcttctgtgc aaccatcggg atttcgacta caagaaggcc
     2041 tgcttcgacg cttacaaccg ctggatagcc gaatactgct cgacgaaccc ggcccgcctc
     2101 ctgggctgcg gccagacggc cgtgcgctcg ccggcggaga cggtgtccga tctggagaag
     2161 attcgcgagc ttggcctgcg cggcgtgatg atgcccggag acccgctgga ggaagactac
     2221 gactcgcaga tctacaaccc ggtctgggaa gctgccatcg acctcggtct gccgctttcg
     2281 tttcacatcc tcacgacccg ccagcaggct ccggtcaggg gtccgcgcat caacagcttt
     2341 ctttcgatca tccggggaaa ccaggacatc atgggcaccc tcatcttcgg cggcgtcttc
     2401 gaccgctatc cggagctcaa ggtggtctgc gtggaggcgg acgccggctg ggtgccccac
     2461 tacatgtacc ggatggacca cgcctacaag cgtcaccgca actggctggc caagggcgtc
     2521 agcctggcca ggcttccgtc cgagtacttc agcgagcacg tctacaccac ctttcaggac
     2581 gactgggtgg cgttcaggct ggtcaacgac atgaactggc ggcgactgct ctgggccaac
     2641 gattttcccc acagcgactc gacctggccg tggtcccagc agatgctcgc agagcagacg
     2701 gcgaacctga ccgaggagca gaaacggggg atcctctgcg acaacgtggc ggaactctac
     2761 cgcatcgatg tggacgccct cgcccaggcc gcctgatgag catacggctg accaagccct
     2821 ggtcgcccgc cagcgccgct gggaagctcc cgggccagat gggcgtctat cagctgggcg
     2881 acgaggcggg caacgtcatc accatcggct acgccggcgg gcgcagcacg ttcggcctgc
     2941 gcggcgaggt cgagactgct cttcagcgtc accctgatgc cgtgagctac cgcgttgagg
     3001 tcaactcggc ctacctgtcg cgatatcgtg agctgctcat ggtgcacctg gcggatttcg
     3061 ggacgctgcc gcccggcaac gaggccctcc cggggctcgg ccggctcagc ccggcttgag
     3121 gggtagccat ggatctcgat ctgagcgagg agcagaacct gctgatcggc acggtgcggc
     3181 gcttcgttcg ggaggagatc ctgcccctgg agcagtctct ggatccggat gcggacgagc
     3241 tcgagcccga tgatcacgaa cgcctgacgc agctgacctg cgaaatgggg ctctacggcc
     3301 tcgacgtccc gccggagttc ggcggcccgg acgtcgatct ggtgacccgg acgctgctcg
     3361 ccatggagat gagccagcat cgcgccggtc tctacgcgcc gtgctacggg gtctttggcg
     3421 gtgcggggct cgctcagctg ttcgaagcga cggaggatca gaaggatcgg tacctcttcc
     3481 ccctgctgcg cggggagaag aagggtttct tcggcctcac tgaaccttcg ggcggcagcg
     3541 atcccgcacg agcgattcag accagggccg ttcgcgacgg cgacgagtgg gtcgtcaatg
     3601 gcagcaagat cttcatcagc ggtgctgacc gggcggattt cggcctcgtg tttgcgcgca
     3661 cggatgccga caagggacgc aacggggtca cctgcttcat cgtggatacg gacacgccgg
     3721 gctttcacgt tcggcgcatc gttcacacgc tgcgcacggc ccgctacgcc accgagctct
     3781 ccttcgagga catgagggtg gcccacggca acgtgctcgg agaggtaaac gggggcttcg
     3841 ccatcgccaa cgatcggctc tccagacagc gcatacccta tgccgccggt tgcatcggcg
     3901 tcgcgatcaa ggctcacgag atggccctgg aatacgttcc ccagcgcgag acgttcggcg
     3961 ccccgctgtc cacacgtcag gccattcagt ggatgctggt ggacaacgag atcgacatac
     4021 ggcaggcctc ctggctcacc ctggaggccg cgcataaggc cgacaagggc gagccgttcc
     4081 gtaaggaggc ggccatggcc aagctggtgg ccaccgaggc gggctcccgg gtggtggacc
     4141 ggtgcatgca gatgttcggc ggctacgggg tgaccaagga cttccccttc gagcgctggt
     4201 atcgggaaat gcgcatccgg cgaatcggcg agggccccaa cgaggtgcag cgacacatca
     4261 ttgcccggga cattctgggg gcgtctctgc gctgaaacgc tttgccggga cggcttccgg
     4321 cgcaatctgt tataaccggc gctcctggga cgctacgagg ctgcaaatgc tacagggacg
     4381 ctacagtgag gccgagctct acagcgagca cgactacgcg cggccacacc agatcgccgg
     4441 gcgccgcctg cacgggggct ttgacgccgc cggcaactac gtgccgcccc gttccaaggg
     4501 gcgctcggag gccatcgagg cgtggacccg ggcgctgcgc gaacgcggcg gagagctgtt
     4561 cgatgccgat gcgagcctgc tttcgggccc gcgaatgccc accgtcgagc agcagcacat
     4621 gctgctccgc gaaggcatcg gccggccgtt ctggaacacc ctgaccgtga ccggcaagat
     4681 cgaggggcgc ggccgggtgc tcgccgagat gcagttcccc gatcttcagg acgtcgtggt
     4741 ggaagacatc tccgacaaag ccgtcggtca cctcaaccgc gggctgctcg ccattcatgg
     4801 catagacgaa ggcggacagc cggagcttgg cctgggcggg cacgacgtga tgtggttcgt
     4861 ggcccgcgac ctggttttcg gccccggggc atacccggac gtggagccgc cggaaagcat
     4921 cgcccggccc gaggccggga aacggtggat gcctgagata tccgctgagc atgaggcgct
     4981 gctcagcttt ctgatgaacc tgctcatcat cgagtttcgg gcggaaatcg ggtttgccgc
     5041 aactcaggac atcctgcggg cagacgacct gttcactgac cggcgcacgc gggcttgcga
     5101 ggcagcggag atcatcgagc gcattcgcgc ggacgaggaa attcacgtgt gctcgctgcg
     5161 gctttatctc ggcgagcttc gccagctcac gctgcgcacg gtagatggcg gcacggtgcc
     5221 ggccgccgag ctgatcgacg gcttctggga cggcctggtt cgatgggcca ccgtggagca
     5281 gccgcggctg gcggcggagc ggcagtacgc tgctatccgg accgagatcc tggagcatcc
     5341 ggacggcgag cgggtcctgg cggcgttcga cgccctcagc gatccggcgg gcctggcgtc
     5401 ggccggctga ttctggccgg acgcgtcagc gcccgtagct cactcccatg cccgcgcgca
     5461 cgtcgttgtt cacgccgtat tggggtatgg ggtatcgaag gccgttgcgg gagagctgct
     5521 ccagcccggc cagcgtcttc ggcaggaact ttggcggcat cgccattagc agctcgtctt
     5581 ccatgacgcc gccgtagcga cgctcggcaa agcacgggat ggagaggctg ggctcgccgg
     5641 tggccagcgc ccggccccac gagtccgcgc aggcggactc gcccacgcat ccccattcca
     5701 gcttgcggta acccgaccac tgcagcccgt tgatgagcag gatcatctgg gccggcgtcg
     5761 cgtagatgag gcagatgtcg ggaggatcga gcctgccgct ggccagcggg ctcacggcca
     5821 tggcctcgta gcgaccatgc ggcaccacgt ccatggcgtg ctggtgagcg ctggcatcct
     5881 cgccggtctc gtaccaaact ccggtcatgc ggtcgccgga gagccagtcg tcgctgcgcg
     5941 gatgcaggcc gatcaccgtg ctgcactggg cgccgacgag gtcctcggcg gtgatgccca
     6001 ccgtccagcc gttgcgggcg gcctggccga cgatctgatc tgtcgtgtga atgtcccgcg
     6061 gccgtcggat gcgcggcact gcctccattt ccccccgcgt ctcgaacagc ttcatcccga
     6121 tcggggtagt gcgcagccgc aacagccggt tcagaccggc caccagctcg gcccagtcgt
     6181 atgaggtttg gtccgtcgtt atcagatcgc tcatgggggg tcctcgtctt ggcgcgttgc
     6241 gggagtcagt cctcgcccac gcgtttgaat cgtggcggcc tgcgctcgat gaacgagcgc
     6301 acgccctcgg tgaagtcctc gcgcttgagg ctctgggcca tccaggcatt gctctcgcgc
     6361 atggcttcgc ccagcgacat gttgagatgc cggtacacct gagccttcat cattttcagg
     6421 gaggtcggcg aggcgttggc ggccaggtcg cgtatgtagt cgcaggcctc gctgacggcg
     6481 gcgccctcgt cgaccaggcg ttcggcgatg ccgatacgct cggcttccgt cccgtcgaat
     6541 tttcgcgagc tccagagtat gtccagggcc cggccgggcc cgatcagccg gggcaggatc
     6601 cagctcaccc catgctcggc tacgaggccg cgctgggaaa aagcggtcgt gaacttggcc
     6661 tggggctcca cgaagcgcat gtcggtgagc atggcaatga cgaagccgag ccccgcgcag
     6721 gccccgccca ccgccgcgat gacgggcttc tgcacggcca tgaggtagcc gtaggtcacc
     6781 tggaagtcct cgcccatccc ggggtccccc acctcggctt cgaggccgga gagatcttcc
     6841 gccgccgcct cgccacccga ggacatacgg tccaggctgc ccatgtccat gccggcgcag
     6901 aatccgcggc ccgccccggt cagcacgatg ccgaccacgc ggccgtcctt ctctgccgct
     6961 cccagcgcgt gccgaatctc ggcgagcatc cggacggtga aagcgttgag gcggtctggt
     7021 cggttcatgg tgatgatcgc gaccggatcc tcgaccgtgt aggtgatttc ttcgtacatg
     7081 tggcgctccg gagcgggccg tttccgatgg aatgtaccgc atcccgcgag ccgagtaatg
     7141 cctggcgggc cttgacggcg gcgctgcaca gcgggaacga tggtttatca tgtcggcttt
     7201 tggaaagtct cgggtgcagt ggtgagccgc tcgctatcat ggttcctggc atttctggtg
     7261 ctgctcggcg ttctggccgt ggctggaagc tggtatctgg gccccagcct ggttgggctg
     7321 gcgttcgacg cggaccgtcg ggaagcgccc tactacgttc tgagcctggc gggccggagc
     7381 gagctggccg acgcggatct gagattcggc tccgatcttg ccgagctgtt cgacgcggcc
     7441 ggtggcaagc tgctatggcg tggcaccacc gagcgggtcg tcaagggccg tctgcaggac
     7501 gagtggctga acgcccagct gtttgccttc ccgcgtggcg caaacttcgt ggagctgaag
     7561 acgggcggcg actaccgcga cctgctggcg gcctacgagg gcaggtcgcg tctgttgctc
     7621 ggcagcccga cccaaccgga tcggatggcc aggggccgta tctgggtcct caacctgatc
     7681 gcggaggatg ccgaggactt gcaggcaacc ggcggcgacc ccctggctgg cttgatgggc
     7741 ggcgtcgcgg gcttcggcgg ctccctggtc tgggatgcga aggtcgcgga cctggacgag
     7801 cgctcgtcct ggaatcgtct ggtgctgctc ggtttcgctc aaccgctgga ggccgaagtg
     7861 tggctttccg atgccggcac ccaaaccgag ctggcgctca gcatggagcg agcgcggcgc
     7921 cgggtcaccc tgctgatgcg cccggccgat ccgatctaga ccctggccga agggcggtga
     7981 aatacccgca ccgtgccgaa ctcgtcgaac tctccgaggt tcggaaacgt ggccgcgtcg
     8041 atggtggtga cctgcacgta ctcaggatcg ggcagctcgc gtattcggga atctgccacc
     8101 agcacctcgt cggcgtggcg ctgagccgtc gagagcagcg ggaggttcgc gcggtcgtag
     8161 agaacgtcgg ccatgagcat caggtcgcat cgcgccggga gggtggcggc gacttccagc
     8221 cgcacgccgt tcatcgccgc gttcacgctg gtggccagcc gcgcatcggc atccgtatcg
     8281 caggcaacga ctcgtgcagc cccggccctc gccgcagcaa tccccgccac gccggatccc
     8341 gagccaaggt cgacgacgga tttcccggcg acccactcgg gattgcgcag caggaactga
     8401 gcaaggccca gaccgctgcc ccagcagaac gcccagtacg cgggtcgttc gaccaccgcg
     8461 ctcatcacct ctcccggtag gggtcccgtg ggaaagtccg cattgatcag ggcgagcctc
     8521 atgcccgagc aatccggaag ggtcaccggc tccaatcggg ccatgggcag ggtcgcgttc
     8581 agccgctgct gcgcatcgcc catgtctgtc ggtcgagcgg ttgccgggcc ggtattcgtc
     8641 atgggcgcac tatatttgcc cgggggccgg gtttcgaggc tagcggggag gaaatctttt
     8701 gaacgagggt tgccggcgag cgggggaggg gccgctcgcc ggcggtgggt atggtgacct
     8761 catacctggt gcgttgactg aggatgcgct cggttacggt gggagcgtcc cgggcaggcg
     8821 gtagggcgct gtctcgggtt gctgtcaccg atggttctcg gggcgcggtc cgttgttatc
     8881 accacctttt tatctgatta tgccgagtat cctagcggga gatggggcgc catgcagtcg
     8941 aggctttgta tcgcgtgtaa ctggctcgta atgctgggcg cgctggcgat cgccgatctt
     9001 gcggctgccg aggctgccga ggcggccggg tgcgacgcgg ccgagtaccg tgcgttcgat
     9061 ttctgggtcg gcgattggga ggtgcgcctg gccgacggat cgctggccgg gcacaacgct
     9121 gtcc
//
LOCUS       DBNHOX010000595         2950 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_562419, whole genome shotgun sequence.
ACCESSION   DBNHOX010000595 DBNHOX010000000
VERSION     DBNHOX010000595.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_562419"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="AC16UZ_13065"
     CDS             complement(<1..506)
                     /locus_tag="AC16UZ_13065"
                     /inference="COORDINATES: protein motif:HMM:NF036554.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HIR6247659.1"
                     /translation="MSVGAFSRSMMRVAAGLLALLVWPAAALGDVSIEGVDEEVRTGI
                     LAFIALDELPCDAPRWWVQRGYRDAVEGSLRALETYGYYRATVQADLVWGEECWTASI
                     DVVPGEPVRLGAVVIEVSLPLADEPDMAALLGNPGLASGERFSHASYETAKGRVLDLA
                     RDLGYLDAA"
     gene            complement(519..1499)
                     /locus_tag="AC16UZ_13070"
     CDS             complement(519..1499)
                     /locus_tag="AC16UZ_13070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HIR6247660.1"
                     /translation="MTVFALIVGIALLLGGGTLLIRGASGVAANAGISPMIVGLTIVA
                     YGTSAPELVVNVVGALENETQIAFGNVIGSNVANLGLILGGTALILPIAIEGRLVRRE
                     LPLLLLGTAVLLVLVLDGPLRAERAVIDRADALVLLLLFSTFIFMTVDQVVRGRQDPL
                     LVGAGQVGAASERGNGGSRNWAEVLLGVAALAGGGHLTIQSGADLAEILGWEKVLVGM
                     VIVAIGTSLPELVTSVIAAVRREPDLCVGNLVGSNIFNGLLVLPISALLRPIDVPAHG
                     VTDVLASFFVAAALIPIFIMGRSRLGRPVGSLLILAYAGYIGYRLATGTA"
     gene            complement(1496..2575)
                     /locus_tag="AC16UZ_13075"
     CDS             complement(1496..2575)
                     /locus_tag="AC16UZ_13075"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HIR6247661.1"
                     /translation="MGKHLNAAFKIAGHTIWPGERVRFDLPGAQLYTHTPLDMPVEVV
                     NGRRAGPTLLICAAIHGDEINGVEIIRRLSALKALSRLHGTLILVPVVNLFGFIHQSR
                     YLPDRRDLNRCFPGSGGGSLGSRVAHLFFEEVVVRSTHIVDLHTGAIHRDNLPQIRAT
                     LDDPVVREMALGFSIPVIVNAGLIDKSLRSEAGKRGIPVITYEGGEALRFDESCIVAG
                     VRGVTNVMRTLGMLPAKHIRRVRAEPYVSRSTVWVRAPIDGIFRTSVRLGARVGVGDA
                     LGSVTSPFATSESTLTSGVGGIIICVNRLPLVNEGEALIHIARFAQAARVEDEIASHG
                     SEIEEDPLYEPGVLEDVDLGPEPGR"
     gene            complement(2568..>2950)
                     /locus_tag="AC16UZ_13080"
     CDS             complement(2568..>2950)
                     /locus_tag="AC16UZ_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR6247662.1"
                     /translation="VQEFVQESAGSDIRCFVVGDKVVAAMSRHAQPGEFRSNLHRGGS
                     AKATKITPTERRTAVSAARAMGLNVAGVDLIRSNHGPLVLEVNSSPGLEGIESATGMD
                     VAGRIVEFIEQNGRPGRTRTRGRG"
BASE COUNT          573 a         1006 c          917 g          454 t
ORIGIN      
        1 gcagcgtcga gatagccgag gtcccgggcc aggtccagaa cgcgtccctt ggcggtctcg
       61 taggaggcgt ggctgaaacg ttcgcccgag gccaggccgg gattgcccag cagcgccgcc
      121 atgtccggct cgtcggcgag cggcaggctc acctcgatga ccactgcgcc aaggcggacc
      181 ggctcaccgg gcacgacgtc gatggacgcc gtccagcatt cttctcccca gacgagatct
      241 gcctgcaccg tggcccgata gtacccgtag gtttccaggg ccctgaggga tccctccacg
      301 gcgtcccgat aacctcgctg aacccaccag cggggagcat cgcagggcag ctcgtccagg
      361 gcgatgaagg caagaatgcc cgttcggact tcctcgtcga ccccttcgat ggacacgtcc
      421 ccaagggcgg ccgcgggcca tacgagcaac gcgagcaggc ccgcggcaac gcgcatcatg
      481 ctgcgggaga atgcacccac ggacattgcg gccgcgacct aagccgttcc ggtggcgagt
      541 cggtaaccga tgtaacccgc ataggccagt atcagcaggg acccaacggg tctaccgagt
      601 cggcttctcc ccatgatgaa gatcggaatc agcgcggcgg caacaaaaaa agatgcgagc
      661 acatccgtca cgccgtgggc aggcacgtcg attggcctca acaaggcgct gatcggcaga
      721 accagcagcc cgttgaagat gttcgatcca accagattgc ccacgcagag gtccggttcg
      781 cgccgcacgg ccgcaatcac cgacgtgacg agctcaggca ggctggtccc gatcgccacg
      841 atgaccattc ccacaagcac cttttcccag ccgagtatct ccgccaggtc cgcgccgctt
      901 tgaatggtga gatggccacc accagccaga gcggccacgc cgagcagcac ttccgcccag
      961 ttacggctcc cgccgttgcc ccgctcggag gcagcgccga cctggccggc gccgaccagc
     1021 agcgggtcct ggcggccgcg aacgacctga tcgacggtca tgaagatgaa cgtcgagaag
     1081 agcagaagca aaaccagggc atccgcccga tcgatcacag ccctctccgc ccgcaacggc
     1141 ccatccagca ccagtaccag aagaacggca gtacccagca gcagcaacgg cagctcgcgg
     1201 cgaaccaggc gtccttcgat ggcaatgggc aggatcagcg ccgttcctcc cagaatcagc
     1261 cccaggttcg cgacgttgga gccgatgacg ttgccgaagg cgatctgcgt ctcgttctcg
     1321 agggccccga cgacgttgac caccagttcc ggcgcgctgg tcccataggc gacgatggtg
     1381 aggccgacga tcatcggaga aataccggcg ttcgccgcaa cgcccgatgc tcccctgatg
     1441 agcagcgtgc ccccaccgag cagcagcgca atgccgacga tcagggcgaa gacggtcaac
     1501 gacccggctc cgggccgagg tcgacatcct cgagcacccc gggctcgtag aggggatctt
     1561 cctcgatttc ggagccgtgg gaggcgatct cgtcctccac gcgagcggct tgggcaaacc
     1621 gagcaatatg aatcagcgct tcaccctcgt tcacgagggg caggcggttg acgcagatga
     1681 tgatgccgcc cactccggag gtcagcgtcg actcgctggt tgcgaaaggc gaagtcacgg
     1741 agcccagcgc gtcgccaacg ccgaccctcg cgccgagcct taccgaagtg cggaatatcc
     1801 cgtcgatggg cgcccgaacc cataccgtgg aacgggatac gtaaggctcg gcccgcaccc
     1861 gtcgaatgtg ttttgccggc agcatgccca gggtgcgcat gacgttggtc acaccccgca
     1921 cacctgcaac gatgcagctc tcatcgaaac gaagcgcctc gcctccctca taggtaatga
     1981 cgggaattcc acgcttgccc gcctcgctgc gcaggctttt gtcgatgagg ccggcattga
     2041 cgatcacggg tatggaaaag cccaacgcca tttcccgaac caccggatca tccagcgtcg
     2101 ccctgatctg cggcaggttg tcgcggtgga tggcgccggt gtgaaggtcg acgatgtggg
     2161 tgctgcggac gaccacctct tcgaaaaaga gatgcgccac tctggagcct agcgagccgc
     2221 caccactccc cgggaagcag cgattgagat cgcgccggtc ggggaggtag cgggactggt
     2281 gaatgaaacc gaagagattc accaccggga ccagaatcag cgttccgtgc agccggctca
     2341 atgccttgag cgccgaaagc cgccggatga tctccacgcc gttgatctcg tccccgtgga
     2401 tggcagcgca gatcagaagc gtcgggcccg cgcgcctgcc gttgacgacc tccacgggca
     2461 tgtccagcgg ggtgtgggtg taaagctgag cgcccggaag atcgaagcgc acgcgctcgc
     2521 ccggccagat ggtgtgaccg gcaatcttga acgccgcgtt gaggtgctta cccacgccca
     2581 cgcgttctgg tcctgcccgg cctgccgttc tgctcgatga actccacgat tcggccggcg
     2641 acgtccatcc ccgtggcgct ctctatgcct tcgaggccgg gcgagctgtt cacttccagc
     2701 accagcggcc catggttgga gcgaatcaga tccacgcccg cgacgttgag ccccatggcg
     2761 cgcgccgccg acaccgccgt gcgccgctcg gtaggtgtga tcttggtggc tttagccgac
     2821 ccgccccggt gcaggttaga gcggaactcg cctggctgcg cgtgccggct catggcggcc
     2881 accaccttgt cgccaaccac gaagcagcga atgtccgacc cggcggactc ctggacgaac
     2941 tcctgcacca
//
LOCUS       DBNHOX010000596         1646 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_690883, whole genome shotgun sequence.
ACCESSION   DBNHOX010000596 DBNHOX010000000
VERSION     DBNHOX010000596.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_690883"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="AC16UZ_13085"
     CDS             <1..236
                     /locus_tag="AC16UZ_13085"
                     /inference="COORDINATES: protein motif:HMM:NF016811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HIR6247663.1"
                     /translation="VEEISDRVLWLEDGSLRDRKSERHSWVRDPVCGMRVDEWSAEYA
                     AEHEGERFIFCSDGCLRRFRDSPERYLTDLAGD"
     gene            complement(269..460)
                     /locus_tag="AC16UZ_13090"
     CDS             complement(269..460)
                     /locus_tag="AC16UZ_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247664.1"
                     /translation="MVESDWEPLRALGFDDQACLEVAHIVGMFNHLTRLADGLGLALD
                     PETQQASASGTALVKVDSQ"
     gene            complement(617..862)
                     /locus_tag="AC16UZ_13095"
     CDS             complement(617..862)
                     /locus_tag="AC16UZ_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6247665.1"
                     /translation="MEREAWVKIPTEKDIGWSATSGDGYAFTFLPAMGRLIMAHPVIG
                     PPFVQMLATVMFGPGSLSRRERELVASVSAAAQDCFY"
     gene            1012..>1646
                     /locus_tag="AC16UZ_13100"
     CDS             1012..>1646
                     /locus_tag="AC16UZ_13100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR6247666.1"
                     /translation="MGDPSTATPEKLSAAGWLAIVAAYLVVPLTLLVVGRDPVWWQAW
                     ALSLLVVVAGVAGRAWAERRHPGLQADRMRFGRGQAVEPWDRVLAPLRALSLLYLPVI
                     VGGLDHRHGGTTPFQLWANVVGLLLCALGYGYAVWALVENRFFTSLVRIQTERGHEVC
                     DSGPYCFVRHPGYAGNLLAVVGIPLALDSLWTFVAAGVALVISLVRTTLEDR"
BASE COUNT          261 a          511 c          535 g          339 t
ORIGIN      
        1 gcgtcgaaga gatctccgac cgggtgctct ggctcgagga tggttctctc cgcgaccgca
       61 agtcggagcg tcactcctgg gtccgcgatc ccgtctgcgg gatgcgcgtt gacgagtgga
      121 gcgccgagta tgcagccgag cacgagggcg agcggttcat cttctgttca gacggctgct
      181 tgcgccgttt tcgggattcg ccagagcggt acctgaccga tctcgcaggc gactgacggc
      241 gccggtcatc gtcgacgacc tcgaggctct attgggagtc gaccttcacg agcgcggttc
      301 ccgatgccga cgcttgctgt gtctcggggt cgagtgccaa gccgagcccg tcggcgagcc
      361 tggtgaggtg attgaacatc ccaacgatgt gcgccacctc gaggcaggcc tggtcatcga
      421 aaccgagcgc gcgaagcggc tcccaatcac tttcgaccat cctggtcgga gtcgcgctga
      481 gcttctcggc caatcggcag agagcgcggg tgcgatcatc tagcttctcg acgtgagacc
      541 actgccgatt tttcaatgcc tcgaccagct cggcatcggc tccttcgaca cgcagaaact
      601 ctgcgtgaga ctgcgttcag tagaagcaat cctgagcagc ggcggaaaca ctggcaacaa
      661 gttcgcgctc tcggcgggac agagagcctg gtccgaacat caccgttgcg agcatctgca
      721 cgaaaggcgg tccgatgaca gggtgagcca tgatgagacg tcccatcgcg ggcaggaacg
      781 tgaaggcgta tccgtctcca gatgttgcgc tccagccgat gtccttctca gttggaatct
      841 tgacccaagc ctccctttcc atgcgccttt ctccttgatg ccgaatctag ccagcgcgaa
      901 ttgacagcgc gaatttgaac ggccccaaac agcatcgaag gagtgtgctg gcaagcattg
      961 agtcggtttg ctcatccggt ggacttctcc gaggtcaggt aggctgggcc catgggtgat
     1021 ccttcgacgg ctacaccgga gaagctttcg gctgcaggct ggctcgccat cgtcgctgcc
     1081 tacctcgtcg tcccgctcac cctgctcgtg gtagggcgcg acccggtctg gtggcaggcc
     1141 tgggcgctct cgctgctggt cgttgtcgcg ggcgtcgctg gccgcgcgtg ggcagaacgc
     1201 cgtcacccgg gcctgcaagc cgaccggatg agattcggcc ggggccaggc agtagaaccg
     1261 tgggaccggg tgcttgcccc gttgagggcg ttgagcctcc tctaccttcc ggtgatcgtc
     1321 ggggggctgg accaccgcca tggtgggacc acgccgttcc agctctgggc gaacgtcgtc
     1381 ggtctgctcc tttgtgccct ggggtatggg tatgcggtgt gggcgctggt ggagaatcgc
     1441 ttcttcacct cgctggtccg catccagacg gagcgcggcc acgaggtctg tgacagcggc
     1501 ccctattgtt tcgtccgcca ccccgggtat gccggcaatc tcctggctgt ggtgggtatc
     1561 cccctggcgt tggattcgct gtggactttt gtcgctgcgg gagtcgcgct cgtcatctcc
     1621 ctcgtccgca ccacgctgga agaccg
//
LOCUS       DBNHOX010000597         1659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_948788, whole genome shotgun sequence.
ACCESSION   DBNHOX010000597 DBNHOX010000000
VERSION     DBNHOX010000597.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_948788"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1300)
                     /locus_tag="AC16UZ_13105"
     CDS             complement(179..1300)
                     /locus_tag="AC16UZ_13105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6247667.1"
                     /translation="MNHRNIVAAIGCLALVACGEAPIDDAAPTARPVKTMIVESPAGS
                     GVRNFPGRIDSANRADLAFRVPGTVARLAASEGAPVNRGQELARLDQTDYEIALRDRQ
                     ATWDRASKDYERSKDLVDKGAISRRDFDRVEANFKSADAALAQAKQNLEYTTLRAPFD
                     GNVARRHVDAFEEINAGQTVYSVIDQSVLEVKIDIPENVIMMLSSRGSEPEEQAGKIP
                     VWAAFDMAPERRFPLTVKEYATRADAQTQTFEVTFALPAPDDVRVLPGMTASVTMDLS
                     RAMDVDSVYYLPVTAVTGSEGLTPRVWAVDEDTMTVREQPIVLGRLVGSSVEVTEGLE
                     PGMRIVTAGAAYLADGMAVTLLAHSEQAEPREEDLPRPS"
     gene            complement(1395..>1659)
                     /locus_tag="AC16UZ_13110"
     CDS             complement(1395..>1659)
                     /locus_tag="AC16UZ_13110"
                     /inference="COORDINATES: protein motif:HMM:NF016300.7"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR6247668.1"
                     /translation="QLDSSDRAAEEEQALERAMQDARAAAARVADGFGVSVGRLLDVH
                     VGQPPVQPMRGVMMEAAAAGDDFSPGQLVISRQLQATFAIETR"
BASE COUNT          242 a          550 c          545 g          322 t
ORIGIN      
        1 tgatgatctt cgcctgcttg atggtgaagt tcggatcctc cagcttcccc atctcgttga
       61 agccgtagat tccgccggcc accagaatga tgaccagcag ccaggagatg accttgttct
      121 cgacggagta tgagccgatg ttcatcgttg ttgttgtccg ttagcgccgc gcgagccgtc
      181 agctcgggcg cggcaggtct tcttcccgcg gctcggcctg ctcgctgtgg gccagcagcg
      241 ttaccgccat gccatccgcc aggtaggcgg ctccggcggt aacgatgcgc atgccgggct
      301 ccaggccctc ggtgacctcg acgctggacc ccaccaggcg ccccagcacg atcggctgct
      361 cgcgcacggt catggtgtct tcatccacgg cccagactct tggggtcaag ccctcgctgc
      421 cggttacggc cgtgacggga aggtagtaga cggagtccac gtccatggcc cgggacagat
      481 ccatggtgac gctggccgtc atcccgggca gaaccctgac gtcgtcaggt gccggcaggg
      541 cgaacgtcac ctcgaaggtc tgtgtctgag cgtcggcgcg tgtggcgtac tcctttaccg
      601 tcagaggaaa gcggcgctcc ggggccatgt cgaaggcggc ccataccggg attttgccgg
      661 cctgttcctc gggctcggac ccgcgcgatg acagcatcat gatgacgttt tccgggatgt
      721 cgattttcac ttccagcacg gattggtcga tcacggagta gacggtctgg ccggcgttga
      781 tctcctcgaa ggcatccaca tgccgtctgg ccacgttgcc gtcgaagggt gcgcgcaggg
      841 tggtgtactc gaggttctgc tttgcctggg ccagcgccgc atcggcgctc ttgaagttgg
      901 cctccacccg gtcgaagtcg cgccgggaga tggccccttt gtccaccaga tccttggagc
      961 gctcgtagtc cttgctggcc cggtcccagg tggcctggcg gtcacgcagc gcgatctcgt
     1021 agtcggtctg gtccaggcgc gcgagctcct gtccccgatt gacgggcgcg ccttccgagg
     1081 ccgcgagcct ggctaccgtg cccggtacgc gaaaggccag gtccgccctg ttcgccgaat
     1141 cgattcggcc ggggaagttg cgaacgccgc tgccggcggg agactcgacg atcatcgtct
     1201 tcaccggccg cgctgtgggg gccgcgtcgt cgatgggcgc ttcgccgcac gcgaccaggg
     1261 ccaggcagcc gatggcggcc actatattcc tgtggttcaa gatccggtcc tccactttcc
     1321 cggggctata ctgcccgcat tcggagccgt tctccagaga ggtccatact gagtctttgg
     1381 ccgccgcgca gaagtcaacg ggtttcgatg gcgaatgtgg cctgcagctg gcgggagatg
     1441 accagctgtc ccgggctgaa gtcgtctccg gctgccgccg cctccatcat cacgccgcgc
     1501 atgggctgga cgggaggctg gcccacgtgc acatccagca gcctgcccac ggagacgcca
     1561 aaaccgtcgg cgacgcgagc agccgcggcg cgcgcgtcct gcatcgcccg ctccagggct
     1621 tgctcttcct ctgccgcccg gtcgctggag tcgagctgg
//
LOCUS       DBNHOX010000598         2857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1269034, whole genome shotgun sequence.
ACCESSION   DBNHOX010000598 DBNHOX010000000
VERSION     DBNHOX010000598.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1269034"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="AC16UZ_13115"
     CDS             <1..260
                     /locus_tag="AC16UZ_13115"
                     /inference="COORDINATES: protein motif:HMM:NF014065.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HIR6247669.1"
                     /translation="RFCMAIGRAGIADLDTGRVAVHLDDVPIALGGLVAPQYEEAAAA
                     RVMARQEFEVHVDLGRGSAEETVWTTDLSYEYVRINAEYRT"
     gene            complement(288..500)
                     /gene="yacG"
                     /locus_tag="AC16UZ_13120"
     CDS             complement(288..500)
                     /gene="yacG"
                     /locus_tag="AC16UZ_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HIR6247670.1"
                     /translation="MTASEPDRTARIVTCPTCGGRVKWTSGNEFRPFCSERCKLMDLG
                     AWASEQHRIPGNEVDDDLLSGDLDPD"
     gene            complement(497..1096)
                     /gene="coaE"
                     /locus_tag="AC16UZ_13125"
     CDS             complement(497..1096)
                     /gene="coaE"
                     /locus_tag="AC16UZ_13125"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003302803.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR6247671.1"
                     /translation="MSRLVIGITGGIGSGKTAVSDRFKALGVTVVDADIASRVIVEPG
                     RPALVAIAEHFGCDVIQADGSLDRAELRRRVFADPDERRWLEQLTHPLINEHIREELE
                     QAESPYAVLVSPLLAETGQSRFCHRILVVDVPEEVQVRRTMARDANDEAQVRKIIEAQ
                     ASREQRLALADDVIVNDSSLEVLDEEVARLHQVYTEMAQ"
     gene            complement(1126..1980)
                     /locus_tag="AC16UZ_13130"
     CDS             complement(1126..1980)
                     /locus_tag="AC16UZ_13130"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR6247672.1"
                     /translation="MGETLLAYGWAGLTFAVWVGLSVGSFLNVVIYRLPIMLSRQWEA
                     QARELLEEEPAETGQERFNLMVPRSRCPACQQPISAWQNVPVFSWLFLKGRCRSCGVR
                     ISIRYPMVEVLTGAATITVLALFGYTWLGLAAAVYTWAMIALTFIDYDTQLLPDQITL
                     PLLWLGLITCHFGGVTDLGSGVLGAVIGYLFLWTTFWAFKLITGKEGMGYGDFKLFAA
                     IGAWLGWQVLPGTILIAATLGLAYALAITLAGKREAAQPIPFGPFLAVAGWVSLMARD
                     NVLTLFAA"
     gene            complement(2030..>2857)
                     /locus_tag="AC16UZ_13135"
     CDS             complement(2030..>2857)
                     /locus_tag="AC16UZ_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR6247673.1"
                     /translation="FCNLVGAGEQSGALETMLDRIATYKEKTESLKAKVRKAMTYPAA
                     VLVVAFIVSGILLIKVVPQFEEVFAGFGAELPAFTQFVINISEFTQQWWFLIVVAIGG
                     TIATFMAARKRSKKLREAIDRIALKAPIFGNIIEKSAVARYARTLATTFAAGVPLVEA
                     LNSVAGSTGNSVYTKAVYQIRDDVSTGQQLNFSMRSTGVFPNMVVQMVAIGEEAGALD
                     EMLDKSASYYEEQVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQLGRVVGG"
BASE COUNT          518 a          899 c          932 g          508 t
ORIGIN      
        1 ggcggttctg catggccatc ggcagggcgg gcattgccga tctcgacacc ggtcgcgtcg
       61 ccgttcacct ggacgacgtg cccatcgccc tgggcgggct cgtggcgccg cagtacgagg
      121 aagccgccgc tgccagggtg atggcaaggc aggagttcga agtccacgtc gacctcggcc
      181 gcggcagcgc cgaggagacg gtctggacaa ccgatctgtc ctacgagtac gtgcgcatca
      241 acgctgagta tcgcacctga tccatggaag cttctcggcc tggccggtca gtccgggtcc
      301 agatcccccg agagcagatc gtcgtccacc tcgttgcccg gaattctgtg ctgctcgctg
      361 gcccaggcgc cgaggtccat gagcttgcac cgctcggagc agaagggtcg gaactcgttg
      421 ccggacgtcc atttgacgcg gcccccgcag gtcgggcagg tgacgatgcg cgcggtccgg
      481 tcgggttcgg aggcggtcat tgcgccatct ccgtgtagac ctggtgcagg cgcgccacct
      541 cctcgtccag cacctccagg ctcgagtcgt tgacgatgac gtcgtcggcg agggccaggc
      601 gctgctcgcg ggatgcctgg gcctcgatga tctttctcac ctgggcctcg tcgttggcgt
      661 cccgggccat ggttcgccgc acctgtactt cctccggcac gtccaccacc agaatgcgat
      721 gacagaatcg cgactggccg gtctcggcca gcagcgggct gacgagcacg gcgtagggag
      781 actcggcctg ttcaagctcc tcgcggatgt gctcgttgat cagcgggtgg gtgagctgct
      841 ccagccagcg gcgctcgtcg gggtctgcga acaccctgcg tcgaagctcc gcccgatcga
      901 gtgatccgtc cgcctgtatg acatcgcagc caaagtgctc ggcgatggcg acgagggctg
      961 gtcggcctgg ctcgactatc acgcgggagg cgatgtcggc gtcgacgacg gttaccccca
     1021 gcgctttgaa ccggtcggag acggcggtct tgccgctgcc gatgccgccg gtgattccga
     1081 tcaccagtct gctcattggg atgtcgtttg aggtcggagg accggtcagg ctgcaaacag
     1141 ggtcagcacg ttatcacggg ccataagcga aacccagccc gcaaccgcca ggaagggccc
     1201 aaatgggatc ggctgcgctg cctcccgttt gccggccagc gtgatcgcca gggcatacgc
     1261 gagccccagc gtcgcggcga tgaggatcgt gcccggcagc acctgccatc cgagccacgc
     1321 tccgatggcc gcgaagagct tgaagtcgcc atagcccatg ccctccttgc cggtgatgag
     1381 cttgaaagcc cagaaggtcg tccagagaaa caggtagccg atcacggcgc cgaggacccc
     1441 ggagcccaga tccgtgacgc cgccgaagtg gcaggtgatc aggcccagcc agagcagcgg
     1501 cagcgtgatc tggtcgggaa gcagctgggt gtcgtagtcg atgaacgtca gcgcgatcat
     1561 tgcccaggtg tacacagcgg cggctagacc cagccacgtg tacccgaaga gcgccagcac
     1621 cgttatggtc gcggccccgg tgagcacctc caccatgggg tagcggatgg agattctgac
     1681 gccgcagctt cggcagcggc ctttcaaaaa gagccagctg aagaccggaa cgttctgcca
     1741 cgccgagatg ggctgctgac aggcggggca gcgcgaacgc ggcaccatga ggttgaaacg
     1801 ctcctgcccg gtttccgctg gttcctcttc gaggagttcg cgcgcctgcg cctcccattg
     1861 ccgtgagagc atgatgggca gtcggtagat gaccacgttg aggaagctgc ctaccgacag
     1921 gcccacccac accgcaaagg tgaggccggc ccatccgtag gcaagaagtg tttcccccat
     1981 gacgtgagcg ctagcccggt cgcatacgat tcgccgtgcc gagcctgggt taacccccga
     2041 cgacccgacc cagctggaag atgggcaggt acatcgcgat gatgaggccg ccgaccagca
     2101 cgccgagaac ggccatgatc atcggctcca tgagcgcggt gaggttgtcc acggcgttgt
     2161 cgacctgctc ttcgtaatag gacgccgact tatccagcat ctcgtccagc gcgccggcct
     2221 cttcgccgat ggcgaccatc tgcaccacca tgttgggaaa cacgccggtg gagcgcatgg
     2281 agaagttcag ctgctgaccg gtgctcacgt catcgcgtat ctggtaaacc gccttggtgt
     2341 aaacggagtt gccggtggat ccggctacag aattcagagc ctctaccaga ggtacgccgg
     2401 cggcgaaggt ggtggccagc gtacgtgcgt aacgggccac ggcggatttc tcgatgatgt
     2461 tgccgaagat gggtgctttc aacgcaatcc tgtctatcgc ctcgcggagt ttcttcgagc
     2521 gttttcgcgc ggccatgaag gtggcgatgg tgccgccgat agcaacgacg atcagaaacc
     2581 accattgctg cgtgaactcc gagatgttta tcacgaactg ggtgaaggcg ggcagctccg
     2641 ctccgaaacc tgcgaagacc tcctcgaatt gaggcaccac cttgatgagc aggatgcctg
     2701 aaacgatgaa cgccaccacc aggacggcgg ccgggtaggt catggccttg cggaccttgg
     2761 ccttcaggga ctccgttttc tctttgtacg tcgcgatgcg gtcgagcatg gtttccagag
     2821 caccggattg ctcaccggct ccgaccaggt tgcagaa
//
LOCUS       DBNHOX010000599         1626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1142136, whole genome shotgun sequence.
ACCESSION   DBNHOX010000599 DBNHOX010000000
VERSION     DBNHOX010000599.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1142136"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1346)
                     /locus_tag="AC16UZ_13140"
     CDS             complement(198..1346)
                     /locus_tag="AC16UZ_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HIR6247674.1"
                     /translation="METAFVQPSRRTLLEHFAAIEDPREPCKVMYAMPEVLLLVVCAT
                     IAGCDDYEEIAEWGEARLDFLRRFLPYHWGVPCADWLRVVMNRIDPELFAACFTAWAD
                     EQRDAGDLDVIAIDGKTSRRSHDRSKGRKALHLVSAWATRERLVLGQEAVDEKSNETT
                     AIPALLARLVVDGALVTIDAIACNPAIARTITDAGADWLLAVKDNQPTLRGEMQSYFD
                     DAPADEVETLTLFDKGHGRLETRRHVVSHAVGWMSGHRRYPGEPRFAGLATIGMVEST
                     VEKAGETSTERRYYISSAALDAARFAEAVRAHWTIENQLHWVLDVQFKEDLSRLRTGN
                     GAHNMAIVRHFALSLVQRVDDRRSIKTRRKRAGWDPEYLRTILQAQSR"
BASE COUNT          288 a          513 c          534 g          291 t
ORIGIN      
        1 aaaacgctgc gcggacatcg atttatcccg ctggttcagt ggacattacg cggtacccgt
       61 ggtggagttg gacgaaaagg accttgatga cctggagcaa ttcgtggacg ggatggaaat
      121 tgaccccgat gacccgcgcg gcgacgagtc gtagcgattt tgctccctcg cggtcatccc
      181 agggcaccga gtccaggtta acgcgattgg gcctgtagga tggtgcggag gtattcggga
      241 tcccatccgg cgcgttttcg gcgtgttttg atgctgcgtc ggtcatcgac gcgttgcacg
      301 agactgaggg cgaagtgtcg gacgatggcc atgttgtggg cgccgttgcc ggtgcgcaac
      361 cgggacaagt cctccttgaa ctgaacgtcg agcacccagt ggagctggtt ctcgatggtc
      421 cagtgggcgc gcaccgcctc ggcgaagcgg gccgcgtcga gggcagcgga ggagatgtaa
      481 tagcgccgct cggtggacgt ctcgccggcc ttctcgacgg tggactcgac catgccaatg
      541 gtggcgagcc cggcgaagcg gggctcgccg ggatagcgcc ggtgtccgga catccacccg
      601 acggcgtggg acaccacgtg gcggcgggtc tcgaggcggc catggccctt gtcgaacagc
      661 gtcagggtct cgacctcgtc ggccggggcg tcgtcgaagt agctttgcat ctcgccgcgc
      721 agggtcggct ggttgtcctt gaccgccagc agccagtcgg cgccggcgtc ggtgatggtg
      781 cgtgcgatgg ccgggttgca ggcgatggcg tcgatggtca ccagcgcgcc gtcgaccacc
      841 agacgcgcca gcagggcggg gatggcggtg gtctcgttgg acttctcgtc gaccgcctcc
      901 tgacccagca ccagacgctc gcgggtggcc caggccgaca cgaggtgaag cgccttgcgc
      961 cccttgctcc ggtcatggct gcgccgcgag gtcttgccgt cgatggcgat cacgtcgagg
     1021 tcccccgcat cgcgctgctc gtccgcccac gccgtgaagc acgccgcgaa cagctcggga
     1081 tcgatccggt tcatcaccac ccgtagccag tcggcgcacg gcacgcccca gtgatacggc
     1141 agaaaacgcc gcaggaaatc gagccgcgcc tcgccccact cggcgatctc ctcgtagtca
     1201 tcgcagccgg cgatcgtcgc gcacaccacc agaagcagca cctccggcat cgcatacatc
     1261 accttgcacg gctcgcgcgg atcctcgatc gccgcgaaat gctccagcag cgtccgccgc
     1321 gacggctgca cgaatgcggt ttccatcggc ttcctcctca aagttgcaag cccaaagaat
     1381 cacagcagac gattcgcctg aatccacctc gcgacaaaaa ttcgaaaccg ttaacctgac
     1441 ctcggtgccc tgaagtcctc ggggattgtc gtaaccgggg cttcgcgaag ctccctgcaa
     1501 agggagtttc agcgatgagg atactttcaa agcggcagct gaaggagctg gttttgtatt
     1561 ctccccaaca cattgcgcgt ctggaggcgg acggccaatt cccaaagagg gtaaggcttg
     1621 gtgcca
//
LOCUS       DBNHOX010000600         2155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_113065, whole genome shotgun sequence.
ACCESSION   DBNHOX010000600 DBNHOX010000000
VERSION     DBNHOX010000600.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_113065"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="AC16UZ_13145"
     CDS             complement(<1..239)
                     /locus_tag="AC16UZ_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247675.1"
                     /translation="MLESLRDFLSQNTWVVEVFAIVFATALVRYAVRRAFERLAGRLE
                     QTHNIYDDALFDAARGPVGVGIWVLGVSWAAEVAGA"
     gene            complement(256..1464)
                     /locus_tag="AC16UZ_13150"
     CDS             complement(256..1464)
                     /locus_tag="AC16UZ_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247676.1"
                     /translation="MAAQPASTDDIEPSSADHSLDLDLGLQLRAFSKSGLDGQSRTQG
                     SVSAQLEYYRAWNEDRSSLTFTPFVRVDGEDDERTHFDVRELFWSRVGPDWDLHLGAK
                     QVFWGVTEFKHLVDVINQTDLVENIDGEDKLGQPMAHLSVVRDWGILDFYLLTGFRER
                     TFPGSDGRLRPPLPVDDDARYESGAGRWRVDGAVRWQRQLGRLTLGIHHFSGTSRAPV
                     LIPETGPAGQAVLRPFYPVIDQTGLDAQAIVGDWAWKLEAVSSSGYEGRYSAFNAGFE
                     RSLVGVLGGRGDLGLVVEYMFDDRDERAFDTLFEHDLALGTRWQMNDVADTQALIGVI
                     WDVKTDEYVVKLEGSRRLGDTWTLLVEGRIFGGSERPMPGVPPSTDPNNRAWPLERDD
                     FLQLELTRYF"
     gene            complement(1527..>2155)
                     /locus_tag="AC16UZ_13155"
     CDS             complement(1527..>2155)
                     /locus_tag="AC16UZ_13155"
                     /inference="COORDINATES: protein motif:HMM:NF028441.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIR6247677.1"
                     /translation="GRGGSEVHRQLRIKQLEVPEDGDKLLVVFDTPKPIKGTALLSVT
                     HKRAPDDQWLYLPAMKRVKKIASQNKSGPFLSSEFAYEDLTVQEIEKFSYRFLGQGQA
                     NGIDCFVVERTPRDDFSGYSRQQVWIDQDELRVLRIEYFDRRDSLLKTLTIDQYARYE
                     DQFWKAGRMYMTNHQTDRSTELFWRNYRFRTGLMDERDFSTNSLLRVR"
BASE COUNT          397 a          720 c          671 g          367 t
ORIGIN      
        1 gccccggcaa cctcggcggc ccaggaaaca ccgagcaccc agatcccgac gccaaccggt
       61 ccccgcgcgg catcgaacag ggcgtcgtcg tagatattgt gggtctgctc aagacgcccg
      121 gcgagccgct cgaatgcccg gcgcaccgcg tagcgcacca gcgcggtggc gaaaacgatg
      181 gcgaacacct ccacgaccca ggtgttctga ctgagaaaat cccgcaacga ttccagcatg
      241 aggccctgcc gcctgtcaga agtaccgggt cagctcgagc tgaagaaagt cgtcccgctc
      301 gaggggccag gcacggttat tcggatccgt ggagggcggc acgcccggca tcggcctttc
      361 ggagcccccg aagatgcgcc cctccaccag cagggtccaa gtgtcgccca ggcggcggct
      421 accttccagc ttgaccacgt attcgtctgt cttgacgtcc catatcacgc cgatcagcgc
      481 ctgggtgtca gccacgtcgt tcatctgcca gcgggtgccg agcgccaggt cgtgctcgaa
      541 gagcgtatcg aaagctcgct cgtcgcgatc gtcgaacatg tactcgacca ccagacccag
      601 gtcgccgcgt cccccgagaa cgccgaccag agagcgctcg aagccggcgt tgaacgccga
      661 gtagcgacct tcgtaaccgc tgctggaaac cgcctcgagc ttccaggccc aatctccgac
      721 gatggcctga gcatccagac cggtctgatc gatcaccgga tagaacgggc gcagcacggc
      781 ctgcccggcc gggccggtct cgggaatcag cacgggggcg cggctcgtcc ccgagaaatg
      841 atgaatcccg agggtcaacc gccccagctg acgctgccag cgaacggcgc cgtcgacccg
      901 ccagcgcccg gcgccggact cgtagcgggc atcgtcatcc accggcaagg gtggccgcag
      961 acgcccgtcg gagccgggaa aagtccgctc gcgaaagccg gtcagcaggt aaaagtccag
     1021 gatgccccag tcgcgcacga ccgagaggtg ggccatgggc tgtcccagct tgtcttcgcc
     1081 gtcgatgttc tctaccaggt cggtctggtt gatcacgtcg accaggtgtt tgaattccgt
     1141 gacgccccag aaaacctgct tagctcccag atgaaggtcc cagtccggac ccacgcggct
     1201 ccagaacagt tcccgcacgt cgaagtgcgt gcgctcgtcg tcctcgccat cgaccctgac
     1261 gaacggcgtg aacgtcaggc tggatcgatc ctcgttccag gcacggtagt actccagctg
     1321 agcgctgacc gatccctggg tccgggactg gccatcgagg ccgctcttcg agaaagcccg
     1381 cagctgaagt cccaggtcga ggtccagcga gtgatcagcg gaggacggct cgatgtcgtc
     1441 ggtagaggct ggctgcgcgg ccaaactgat cccgccggca gcgagcagcg cagctgccag
     1501 gcaccaggga cggggccctt ggcaagtcat cgcacccgca gcaggctgtt ggtagaaaaa
     1561 tcgcgctcgt ccatgagccc cgttcggaac cggtagttgc gccagaagag ctcggtgctc
     1621 cggtccgtct gatggttggt catgtacatc cggccggcct tccagaactg atcctcgtaa
     1681 cgggcatact gatcgatggt cagggtcttg agcaggctgt cccgccggtc gaagtactcg
     1741 atccgcagca cgcgcagctc gtcctgatcg atccagacct gctgccgcga gtagcccgag
     1801 aagtcgtcgc gcggggtacg ctccaccacg aagcagtcga tcccgttagc ctgcccctgg
     1861 ccaaggaagc ggtagctgaa cttttcgatc tcctgaacgg taaggtcctc gtaggcgaac
     1921 tcgctgctca gaaacggtcc cgacttgttc tggctggcga tcttcttgac tcgtttcatg
     1981 gcaggcaggt agagccactg gtcgtccggg gcgcgtttgt gggtgacgct gaggagcgcg
     2041 gttcccttga tgggtttcgg ggtgtcgaaa accaccagca gcttgtctcc gtcctccggc
     2101 acttccagct gcttgatccg cagctggcga tgcacctccg atcctccgcg gccgc
//
LOCUS       DBNHOX010000601         2859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_755519, whole genome shotgun sequence.
ACCESSION   DBNHOX010000601 DBNHOX010000000
VERSION     DBNHOX010000601.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_755519"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(369..839)
                     /locus_tag="AC16UZ_13160"
     CDS             complement(369..839)
                     /locus_tag="AC16UZ_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247678.1"
                     /translation="MAEQAGLIDVPLLVEALTWLGVSTPEGQELAAARVGHLTNYLIR
                     QVLHGKEQHRQRAAAARSAEAQRGAGQQGHYSPAELRAFNAGRAFAERIARAEQNARR
                     RDLCQMALHGLADDGVSQEAVLHRIVAMLYPDLAGLVDQDPELARRALNALQPY"
     gene            complement(796..1368)
                     /locus_tag="AC16UZ_13165"
     CDS             complement(796..1368)
                     /locus_tag="AC16UZ_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247679.1"
                     /translation="MLDYLSSQVGFDAAATVAAWWGGTRLYVPSEADPSHYIARVAGE
                     PVLAALVDEWGGGYVWVSNPLHAAQAARNRHVAELVQAGHTDEQIAEELHLTSRRIKQ
                     IKQKLAEDGIHGFPREKSSGKIRGENPQEKVGSKMPRKNRGRKSVSGNDWRIEYENFR
                     HRSRARRSRHAPLRRPLGRASGPHRRAAPG"
     gene            complement(1750..>2859)
                     /locus_tag="AC16UZ_13170"
     CDS             complement(1750..>2859)
                     /locus_tag="AC16UZ_13170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HIR6247680.1"
                     /translation="MVTLAAASPRVMVPNAELNKHAQYLGAPNGVIDLVSGQLLPPDP
                     GLRLTQSVSVPYDPEATCPVFERVVREVFSGDEEMVEFFQRLVGYSASGKPAQQLLVI
                     PFGSGANGKSTVLNAIRRALGGYARSADASSFVSDGRSAASAGGARSDLVRLAGSRFV
                     YVSEPDEGGELREGTVKSLTGDDAISARELYSKTTIEIRPTWTMWMPTNHRPIIKGTD
                     LGIWRRICLLPFSRNFENDPHVKRDLNLADKLDAETAGILAWIVRGYQAYLTDGLKEP
                     AAVTSAKGSYKSSMDTLAGWLNECCDIGSGLVTSHSDLWASWEQYARRNGMLTYCKSS
                     IALSRRLEGRFERGPTTGGMVRWAGIRVKSDFNPV"
BASE COUNT          472 a          936 c          864 g          587 t
ORIGIN      
        1 cgcaggccac ctggggctcc acgggtcggc gcaggccacc tggggcacca cgggtcggcg
       61 caggccacct ggggctccac gggtcggcgt ctgcggtatg tagcgccgtc aatcctgttt
      121 ctaggaaaac catccggttc taaaatgaaa tccgaaaaag catccggttc aaaatccaat
      181 cggcaaacct agccggatca ttttagaatc tggaaacgtc accggatcaa attccagttt
      241 gtgcgtccag cagctagcgc caagtgaaaa cggccccgta gagggccgtt ctgcgtttca
      301 ggctacctgc ggccatcggg ggcgccgatc gcggctctgg agggccgtct cgtgcgtctg
      361 cgggtgcttc agtagggctg cagggcgttg agggcgcggc gggcaagctc cgggtcttgg
      421 tccacaaggc cggcgaggtc gggatagagc atcgccacga tgcggtgcag cacggcctct
      481 tggctgacgc cgtcgtcggc cagtccgtgc aaggccatct ggcaaaggtc acgccggcgt
      541 gcgttctgct cggcgcgggc aatgcgctcc gcaaaggccc gtcccgcgtt gaaagcgcgc
      601 agttcggcag gcgagtagtg gccctgctgg ccggcgcccc gttgggcttc cgcagaccgt
      661 gctgcagccg cgcgttgccg gtgctgctcc ttgccatgca gcacctgtcg gatcaggtag
      721 ttggtcaggt gaccgacccg cgctgcagcc agctcctgcc cctctggcgt gctgacgccg
      781 agccacgtga gcgcctcaac caggagcggc acgtcgatga ggcccgcttg ctcggccaag
      841 agggcgtcgt aggggagcat gcctgctgcg ccttgcgcgg ctgcggtgtc gaaagttttc
      901 atactcaatt ctccaatcat ttccgctgac agattttcta ccccggtttt tccggggcat
      961 tttcgacccc actttttcct ggggattttc accccggatt ttcccggagc ttttttcccg
     1021 cgggaatccg tgaatgccat cctcggctag tttttgcttg atctgcttga tgcgccgact
     1081 cgtcaggtgc aattcctcgg cgatctgctc gtcggtgtgg ccggcctgca ccagctcggc
     1141 cacgtggcgg ttgcgggccg cctgggccgc gtgcagcggg ttgctgaccc acacgtagcc
     1201 gccgccccac tcgtccacca gggcggccag gacgggctct ccggccacgc gggcgatgta
     1261 gtggctgggg tcggcctcgc tgggcacgta gaggcgcgta ccgccccacc atgcggccac
     1321 ggtcgcggcg gcgtcgaagc cgacttgcga cgagaggtag tcgagcacgg attcttccat
     1381 gttgcgtctg ctttctgtcc cagcttttct ggggcgtttc ggggtcgaac ttgttgaaaa
     1441 acacgagtgg tccttctgac cactttttac tttctttttc tttacgtcac ggggaccaaa
     1501 tattagagtt gccagatgga ccactcgggc ctctttgcag ccctgttata ccacttagca
     1561 gagtctgtta gctagtttta acacgttgta cttagctcat caagttgcta attttaacgt
     1621 gttctactaa cttcatgcag gtaacgcccg gtacttcgga tttttcacaa gtacgcctcg
     1681 gtaccgatcg aacctgcact ttttgcgaac tcgctttttt cccgaattcg caaaatcgac
     1741 gacttccggt cagaccgggt tgaagtcgga cttcacccgg atgccggccc accggaccat
     1801 cccgccggtg gtcggacccc gctcgaaccg gccctccagc cgcctagaca gcgcgatcga
     1861 cgacttgcag taggtcagca tcccgttgcg ccgagcgtac tgctcccagc tcgcccacag
     1921 gtctgagtgc gacgtgacca gcccgctccc gatgtcgcag cactcgttca accagccggc
     1981 cagcgtgtcc atgctgctct tgtagctccc tttggccgag gtcacagctg ccggctcctt
     2041 cagcccgtca gtgaggtacg cctgataccc ccggacaatc cacgcgagta tcccggcagt
     2101 ttctgcatcc aatttgtctg cgagattcag gtcgcgtttc acatgggggt cgttctcgaa
     2161 gttccgcgag aaaggcagca ggcagatgcg gcgccagata cccaagtccg tccccttgat
     2221 aatcggcctg tggttggtcg gcatccacat cgtccacgtc ggcctgatct cgatggtggt
     2281 tttcgagtac agctcgcgcg cggagatcgc atcgtcgccc gtcaagcttt tgaccgtccc
     2341 ctcccgcaac tcgccgccct cgtcgggctc gctgacgtaa acgaaccgcg acccggccag
     2401 gcgcacgagg tcgctccgcg caccgcccgc actggccgca gaccggccgt ccgacacgaa
     2461 gctgctcgcg tccgcggacc gcgcgtaccc acccaacgcc ctgcggatcg cgttcaggac
     2521 cgtgctcttg ccgttggcgc cggacccgaa tgggatcacc agcaactgct gggccggttt
     2581 tccggaggcg gaatagccca cgagccgctg gaagaactcc accatctctt cgtcgccgga
     2641 gaacacctcg cggaccaccc gctcgaacac cggacaggtg gcttcagggt cgtagggcac
     2701 cgacacgctc tgcgtcaggc gcaggcccgg gtcgggaggc agcaactggc cgctgacgag
     2761 gtcgatcaca ccgttggggg cacccaggta ctgcgcgtgc ttgttcaact cggcgttggg
     2821 caccatgacg cgcggactgg ccgcggccag cgtcaccat
//
LOCUS       DBNHOX010000602         9301 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1334567, whole genome shotgun sequence.
ACCESSION   DBNHOX010000602 DBNHOX010000000
VERSION     DBNHOX010000602.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1334567"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="AC16UZ_13175"
     CDS             complement(<1..259)
                     /locus_tag="AC16UZ_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247681.1"
                     /translation="MAVPEGRRGLWGPASLLARTNLILAVGSLVIALIAIAALNVFVI
                     EPIAERSADDEAALLVLSAQTWVELPPAARPYFEIELAQNHD"
     gene            complement(246..956)
                     /locus_tag="AC16UZ_13180"
     CDS             complement(246..956)
                     /locus_tag="AC16UZ_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6247682.1"
                     /translation="MDAEGAKLLVVDDDPEIRELAQAYLSQQGFEVDCVDSGEAMDAY
                     LESNEVDLIILDLMLPGEHGLSIARRLKTDGDVPIIIVSAQGEEIDRIVGLEVGADDY
                     IGKPFNPRELLARVRAVLRRAQRTGQASSAENRVCFGSFELDLSAHQLTADGKTVPLT
                     SGEFDLLAILVSHPNKVLDRDRILDLLTGAERSPFDRSIDVRVTRLRAKIEPDPSTPV
                     FIKTIWGKGYMFCPDGRA"
     gene            complement(966..1388)
                     /locus_tag="AC16UZ_13185"
     CDS             complement(966..1388)
                     /locus_tag="AC16UZ_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247683.1"
                     /translation="MLFTSLSGGAFAADEAVRAGDAASIPNVYAAKSDQELTALAAQW
                     ESLDTAERRALLTEVKMRMARQRGPDGTIRITFQRRYGRIIRNPDGSVVRIETNVVRV
                     RPGAAGNQTYGVGFEQRTADGQREEQSPPVITVKDPSP"
     gene            complement(1652..2836)
                     /locus_tag="AC16UZ_13190"
     CDS             complement(1652..2836)
                     /locus_tag="AC16UZ_13190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6247684.1"
                     /translation="MKDVVIVDSVRTGLAKSFRGSFNLTRSDDMLAHCIDALLDRNGG
                     VSPHEVEDVVVGAASHVGEQDGNLARLAVVLSKLPITTAGLTINRFCSSGLQSIAIAA
                     NQIATGQTQVAIAGGVDSISMRTRQEAAKAELNKRLVEEKPDIYMAMGNTAEVVARRY
                     QVTREAQDEYALQSQQRYAAAAEAGYVAEEIVPMAATMLKVDKETGEESRVDVVVDRD
                     ECNRPTTTLEGLASLPPAFEEDGSVTAGNASQLSDGASMTLLMSAERAAQLGLEPLGI
                     YRGFTVAGCEPDEMGIGPVFAIPRLLENAGLKQDDVDLWELNEAFASQCLYCRDALGI
                     DNEIYNVNGGSISIGHPFGMTGSRMTGLVLRELKRRGKRYGVVTMCIGGGQGAAGLFE
                     AA"
     gene            complement(3125..3469)
                     /locus_tag="AC16UZ_13195"
     CDS             complement(3125..3469)
                     /locus_tag="AC16UZ_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247685.1"
                     /translation="MSTIKNWMMALLGVVVLVGFVGCGQDAGSGPADAPAAAEEAAAD
                     ETAGGFIQPAAEAAEEAMDEGMEALEEAADETATEAAAEAAEMMEEAAAEAEEAAESA
                     MGEMNITPPGSN"
     gene            3648..4490
                     /locus_tag="AC16UZ_13200"
     CDS             3648..4490
                     /locus_tag="AC16UZ_13200"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR6247686.1"
                     /translation="METITLVDGLCFGEGPRWRDGRLWLSDMHAQQVIAVDGDGHVQV
                     VVEVPNLPSGLGWLPDGDLLIVSMRDRRVLRHDGARLHTHADLSQLASFDCNDMVVDV
                     HGRAYVGNFGFDLHGGEAPRAAELICVEPDGTARIVADDLLFPNGTVITPDGNTLIVA
                     ESWGGRLTAFDIDQAGNLSNRRDWAKLPDGAVPDGICLDSDGGIWTASPATNECLRQI
                     EGGTVTHRVPVNQGAFACMLGGDDGRSLFILTAGSSAPDACRAARSGRVELVTAPFPH
                     AGWP"
     gene            4529..5683
                     /locus_tag="AC16UZ_13205"
     CDS             4529..5683
                     /locus_tag="AC16UZ_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6247687.1"
                     /translation="MYENYESPWLNDELRLLQDAAAKFFQQEFVPLNEKWINQGKVDR
                     EAWYKAGEAGLLCAEMPVEYGGGGGDYRHETVIMEEQLATGVTGFGNQVHSSIVAPYI
                     LHYGSEDQKQRWLPKMATGELVGAIAMTEPNTGSDLQAVRTSAIRDGDEYVINGSKTY
                     ISNGQHADLVIVVAKTDPAQGAKGISLIVVETGKSPGFERGRNLKKLGQKAADTSELF
                     FADCRVPVTNRLGEEEGRGFVQLMQQLPQERLNIGQAAVVAMERAIGITLDFVRERRA
                     FGQRILDFQNTRFKLSECKTEALIARTFVDQCVLKLVNGELDAVTASMAKYWCTQKQC
                     EIIDECLQLHGGAGYMDEYEISRMYADARVQTIYGGTNEIMKELIGRSLG"
     gene            5691..6971
                     /locus_tag="AC16UZ_13210"
     CDS             5691..6971
                     /locus_tag="AC16UZ_13210"
                     /inference="COORDINATES: protein motif:HMM:NF018913.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247688.1"
                     /translation="MIQYWRFYWPLALTGLAMVLAVQFQNAALARFPEAVTELAVFAL
                     AYSAYALFHASLNFVAQLSNVYARSPQGTSRSWRFATAASACIMLPLLALAHTDYGAD
                     ALGSIYGIDDGLVERVTEYLAYLAPLLLISAQRFYYNGLLVQARLTGWVTTLTVVYLT
                     SVLVGLIIGFTLGLKPVHVLVGAEGIALLIQLALSVLIKASRYAPPADPEHADLSYGE
                     LLRFYLPVSTTAVMFALSRPILYAFVARTPNGVLAIAAMRVAFDFATIFQQAANQFRH
                     FFVTFGWNDLGIKRRFLTVICAGITAIMLAVALTPLSELIWGKLMGIPEPVRDIAEHV
                     LVVMCLMPALIVFRNYYHGRLMVERRTSGMAAGGILRVIAILLAAQACTMMGWLNYLS
                     ASFILILGFLVEALVALVAAQKGQGLRANATANM"
     gene            complement(6926..7285)
                     /locus_tag="AC16UZ_13215"
     CDS             complement(6926..7285)
                     /locus_tag="AC16UZ_13215"
                     /inference="COORDINATES: protein motif:HMM:NF016161.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HIR6247689.1"
                     /translation="MLKSIHLILAYLTVLGFVVRGIWSLVDSPLKNQKWVRISPHVID
                     TALLVLGVALAVQLSVSPLEGWLAAKLAGLLAYIGFGVLTMRASSTPLKLAGFAGALA
                     SVGYIFAVAFARSPWPF"
     gene            7373..8836
                     /locus_tag="AC16UZ_13220"
     CDS             7373..8836
                     /locus_tag="AC16UZ_13220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR6247690.1"
                     /translation="MNIDQAIDQAMRPLADAVSGFIFFSVPVLGTQIPLIVVWLILGG
                     LFFTVYLRFVNIRGFAHAIGLVSGRYHNDQHPGEISQFQALTTAVSGTVGIGNIAGVA
                     VAISMGGPGATLWLIIAGLLGMSTKFAECTLGLKFRRHHEDGSVSGGPMYYLERGLAE
                     RNWPRVGRGLGLFYAASMVIGCLGIGNMFQSNQAAAIFIDVSGGDASYFQDKAWLLGL
                     IMAGAVGLVIIGGIRSIARTTSRLVPGMALLYVISALAIIAINAAELPAAFAAIWAGA
                     FSPEGIAGGLIGVMIIGFRRAVFSNEAGLGSAAIAHSTAKTERPVSEGYVALLEPFID
                     TVVICTMTALVIITTVYEPGLTSGNVSGIELTTRAFASTLSWSPIPLSIAAILFAFST
                     MIAWSYYGLKAFTYLVGHHRVADLGFKIFFLVFVVLGSSVQLGALVDLSDALVFVVAI
                     PNLLGLYLLAPTVRAELRAYESNMPVPNGAAPAPDST"
     gene            8891..>9301
                     /locus_tag="AC16UZ_13225"
     CDS             8891..>9301
                     /locus_tag="AC16UZ_13225"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6247691.1"
                     /translation="MEGPTSHVYFSQRLRLHYVDWGNPDAPPMLMIHGGRDHCRNWDW
                     VARSLRDRYHIIAPDLRGHGDSQWMLGGSYNQIDYVYDIAQLLTQKDMTPVTIIGHSL
                     GGSIALTYSGLFPETVKKLVAIEGMGPPPEFIKER"
BASE COUNT         1623 a         3087 c         2860 g         1731 t
ORIGIN      
        1 gatcgtgatt ctgcgccagc tcgatctcga agtacgggcg ggctgccggc ggcaactcta
       61 cccaggtctg ggcagaaagc acgagcaggg ccgcttcgtc gtccgccgag cgctccgcga
      121 tgggctcgat gacgaaaacg ttgagcgcgg cgatggcgat cagcgcgatg accagcgaac
      181 cgactgccag gatgaggttg gtccgtgcga gcagagacgc cggaccccag agcccccgcc
      241 gcccctcagg cacggccatc ggggcagaac atgtaccctt taccccagat ggtcttgatg
      301 aataccggcg tggagggatc cggctcgatt ttcgcccgca gccgggtcac ccgcacgtcg
      361 atgctgcgat cgaagggcga ccgctcggct ccggtcagca gatccaggat gcggtctcgg
      421 tccagaacct tgttgggatg ggagacgagg atcgcgagca gatcgaactc tcccgacgtg
      481 agcggtaccg tcttgccatc tgccgtcagc tgatgagccg aaaggtccag ctcgaagctc
      541 ccgaaacaca cgcggttctc tgcggacgac gcctgcccgg ttcgctgagc gcggcgcagc
      601 acggctcgaa cccgggcaag cagctcgcgg gggttgaacg gcttgccgat gtagtcgtct
      661 gcgccaacct cgagcccgac gatgcggtcg atctcttcgc cctgggcaga cacgattatg
      721 atggggacat cgccgtcggt cttgagccgg cgggcgatcg aaaggccgtg ctcaccgggc
      781 agcatgagat cgagaatgat gagatccacc tcgttggatt ccaggtaggc gtccatggcc
      841 tcgccgctgt cgacgcagtc cacctcgaat ccctgctggg agagataggc ctgtgccagc
      901 tcacgaatct ctgggtcgtc gtcaacgact agaagcttgg caccttcagc atccacggcg
      961 ggggcttacg gtgagggatc cttgaccgtg atgaccggcg gcgactgctc ctcccgctgg
     1021 ccgtctgcgg tcctttgctc gaagcctacg ccgtaggttt gattgcccgc cgcgccgggg
     1081 cggactcgaa ccacgttggt ctcgatccga accacggatc cgtccggatt gcggatgatg
     1141 cggccgtaac ggcgctgaaa cgtgatgcgg atggtgccgt ccggaccgcg ctggcgcgcc
     1201 atacgcatct tgacctcggt caacagggcc cggcgctcgg cggtgtccag ggattcccac
     1261 tgggcggcga gagccgtcag ctcctggtcg gatttggctg catagacgtt cgggatgctg
     1321 gccgcatcgc ccgcacgcac agcctcgtcg gcggcgaaag cgccgccgga cagtgacgtg
     1381 aagagcaaaa cgctcagggt caggcttctg atgaacgtaa cccctgttcc attcggagct
     1441 ccgaccgcat ccgcgcaccg gatgctccca ttttccgccc cgtgatgatt ggaccactat
     1501 aaatgcttat ggtgccggca gcctctacgt gtcctacaag aacgcgcaca ggcctcgcga
     1561 attatcccca gagcgacggc aattctgact ctgcgctgtt tcagcgccat ttcccttggg
     1621 tttcaatttg ttacttaacc ccgggggctg atcaggctgc ctcgaacaga cctgctgcac
     1681 cctggccacc gccaatgcac atggtgacga ctccgtacct cttgccacgc cttttcagct
     1741 cgcggagaac caggcccgtc atgcgcgagc cggtcatccc gaacggatgg ccgatgctga
     1801 tgctgccgcc gttgacgttg taaatctcgt tgtcgatgcc cagcgcatcc cgacaataca
     1861 ggcactgcga cgcgaacgcc tcgttgagct cccacagatc gacgtcatcc tgcttgagac
     1921 ccgcgttttc cagcagccgc gggatggcga acaccgggcc gatgcccatc tcgtcgggct
     1981 cgcagccggc cacggtgaag ccccggtaga tgcccaacgg ttctaggccc agctgcgctg
     2041 cgcgctcagc gctcatgagc agcgtcatgg acgcgccgtc ggagagctga gaagcatttc
     2101 ccgcagtgac ggagccatct tcctcgaagg ccggcggcag cgatgccaga ccttccaggg
     2161 tggtggtggg gcggttgcac tcgtcccggt cgacgacgac gtccacgcgg ctttcttcac
     2221 ccgtctcctt gtccaccttg agcatggtgg ccgccatggg cacgatctct tcggcgacat
     2281 atccggcctc agcggcggcc gcgtatcttt gctggctctg cagggcatac tcgtcctggg
     2341 cttcgcgggt cacctgatag cgccgcgcga ccacctcggc ggtattcccc atggccatgt
     2401 agatgtccgg tttctcctcc accagccgct tgttgagctc ggccttggcc gcctcctgac
     2461 gggtacgcat ggagatcgag tcgacgccgc ctgcaatggc gacctgagtc tggccggtcg
     2521 caatctggtt ggcggcgatg gcgatggact gcaggccgga cgagcagaag cgattgatgg
     2581 tcaggccggc ggtcgtgatc ggcagcttcg acagcaccac ggccagtcgg gccaggttgc
     2641 cgtcctgctc gccgacgtgg ctagccgccc ccaccacgac gtcttccacc tcgtgcggcg
     2701 atacgccgcc gttcctgtcg agcagcgcgt cgatgcagtg cgcgagcatg tcgtcggagc
     2761 gggtgaggtt gaagctccca cggaaggatt tggcgagccc ggttcgaacg ctatcgacga
     2821 tgaccacgtc tttcatgatg ttcccctttc aagttgttcg ctcaacgcag gccctgggtt
     2881 ttgcagcgcc ccgacaaaag tgccccggga gcgtaccaga aaaaaaaaga cacggtctcg
     2941 gctggctggg cccgcaccct cgtcatacgc gcgccaatga cgctgaccgc ttcgctgcag
     3001 ggcaaacaga aacggcccga ggctggcgca ccgcgccgcg ttcggaccgt tacctgatca
     3061 cgagcgggcc agacttcgaa cagttcgaaa ctgcactgac ccgttggctg gaagtggaag
     3121 gcggctagtt ggagcccggc ggggtgatgt tcatctcccc catggccgat tcggctgcct
     3181 cttctgcctc cgcggctgct tcttccatca tctcggctgc ctctgctgca gcttcggtgg
     3241 cggtttcgtc agcggcttcc tcaagcgctt ccatcccctc gtccatagcc tcttcggctg
     3301 cttcagcggc aggctgaatg aacccacccg cggtctcatc cgcggctgcc tcttccgccg
     3361 cggcgggcgc atcagcgggt ccggaccctg cgtcctggcc gcagccgaca aacccgacga
     3421 gaacgacaac gccgagcagc gccatcatcc agttcttgat cgtactcatc tatccctcct
     3481 cgttgctttc gacccagggc cagaccctgc aggctgagca gacgcaggcc ttattgccac
     3541 cgtcattccc cagcggtcga ggtattcgca agactacaga aaaaggtctg gccaaccaac
     3601 ctgtacctgt cggcgtgcta agctaagcgg ccacctctgg agggtccatg gaaacgatca
     3661 ctttggtcga tggcctgtgc ttcggcgagg ggccgcgctg gcgagacggc cgtctgtggc
     3721 tgtctgatat gcacgctcaa caggtgatcg ccgtggacgg cgacggccac gttcaggtcg
     3781 tggtcgaggt gccgaacctc ccctccggcc tgggatggct cccggacggc gacctcctga
     3841 tcgtctccat gcgcgatcgg cgcgttcttc gccacgacgg cgcccgcctg cacacccatg
     3901 ctgacctgtc acagctggca agtttcgact gcaacgacat ggtggtggac gtccacggta
     3961 gggcctacgt gggaaacttc ggtttcgatc ttcacggcgg agaagcgccc agggccgcgg
     4021 agctaatctg cgtggaacct gacggaaccg cgaggatcgt cgcagacgac ctgctctttc
     4081 ccaacgggac ggtcatcacg ccggacggca acacgctgat cgttgcagaa tcctggggcg
     4141 gccgactgac cgctttcgac atcgaccagg ccggcaacct gtcgaaccgc agagactggg
     4201 ccaagctccc cgacggcgcc gtgcctgacg gcatctgcct ggattccgac gggggaatct
     4261 ggactgcctc gcccgcgacc aacgagtgcc tgcgccagat cgaagggggc accgtcacgc
     4321 accgggtccc ggtgaaccag ggcgcttttg cgtgcatgct cgggggagac gacggccgct
     4381 ccctcttcat cctcaccgcc gggtcatcgg cgcccgacgc ctgccgtgcg gcccgctccg
     4441 gacgcgtgga gctcgttacc gccccgttcc cgcacgccgg ctggccgtaa aagccgcgca
     4501 accgactcac acgaggaaac aagacgtgct gtacgaaaac tacgagtcac cctggctgaa
     4561 cgatgagctt cgcctgctcc aggacgccgc agcgaagttt tttcagcagg agttcgtgcc
     4621 gctgaacgag aagtggatca accagggcaa ggtggaccgt gaagcctggt acaaggccgg
     4681 cgaggcaggc ctgctctgcg cggagatgcc cgtcgagtac gggggcggtg gcggcgacta
     4741 ccgccacgag accgtcatca tggaggagca gctcgccacc ggggtgaccg gcttcggcaa
     4801 ccaggtccac agcagcatcg tcgccccgta catcctgcat tatggctccg aagaccagaa
     4861 acagcgctgg ctgccgaaga tggccaccgg cgagctcgtc ggcgccatcg ccatgaccga
     4921 gcccaatacg ggctccgatc ttcaggccgt gcgcacgtcg gcgatccgcg acggtgacga
     4981 gtacgtcatc aacggctcca agacctacat ttccaacggc cagcacgccg atctggtcat
     5041 cgtcgtggca aagaccgatc cggcccaggg cgcgaagggc atatcgctga tcgtggtgga
     5101 gacgggcaag agtccgggtt tcgagcgggg acgcaacctc aagaagctgg gacagaaggc
     5161 cgccgacacc tcggagctgt tctttgccga ctgccgggtt cccgtcacga accgcctcgg
     5221 cgaggaggaa ggacgaggct tcgtccagct gatgcagcag ctgcctcagg aacgcctgaa
     5281 catcggccag gcagccgtgg tggccatgga acgcgccatc gggatcacgc tcgacttcgt
     5341 cagggagaga cgcgccttcg gtcagcggat cctggatttc cagaacacca gattcaagct
     5401 gtccgagtgc aagacggagg ccctcatcgc ccgcaccttc gtggaccagt gcgtgctcaa
     5461 gctcgtcaac ggcgagctcg acgccgtcac ggcatccatg gccaagtact ggtgcaccca
     5521 gaagcagtgc gaaatcatcg acgagtgcct gcagctgcac ggcggtgccg gctacatgga
     5581 cgagtacgag atctcgcgga tgtacgccga tgcaagagtg cagacgattt acggcggcac
     5641 caacgagatc atgaaggagc tcatcggccg cagcctggga tagttccagg atgatccagt
     5701 actggcgctt ctactggcct ctcgccctca ccggcctggc catggtactg gccgtgcagt
     5761 tccagaacgc ggcgctcgcc cgtttcccgg aagccgttac cgagctggcc gtgttcgcgc
     5821 tggcatacag cgcgtacgcc cttttccacg ccagcctgaa cttcgtcgcg caactctcga
     5881 acgtctacgc tcgcagcccc cagggcacct cacgcagctg gcgattcgcc accgctgcga
     5941 gcgcgtgcat catgctgccg ctgctcgcgc tcgctcacac cgactacggc gccgacgcgc
     6001 tgggctcgat ctacggcatc gacgacgggc tcgtcgagcg ggtcacggaa tacctcgcct
     6061 acctcgctcc tctcctgctg atcagcgcgc agcgcttcta ctacaacggt ctgctcgtgc
     6121 aggcccgatt gaccggctgg gtgaccacgc tgaccgtcgt ctacctcacc agcgtgctgg
     6181 tcgggctgat catcggcttc acgctgggac tcaagcccgt gcacgttctg gtcggcgcgg
     6241 agggcatcgc cctgctgatc cagctggcgc tgtccgtgct gatcaaagca agccgctacg
     6301 cccctccggc agacccggag cacgcggacc tgtcctacgg cgagctgctt cggttttatc
     6361 tcccggtgtc gaccacggcg gtcatgttcg cgctgtcacg gcccatcctc tacgcgttcg
     6421 tggcacgcac gcccaacgga gtgctcgcca tcgccgccat gcgcgtagcg ttcgacttcg
     6481 ccaccatttt tcagcaggcc gccaaccagt ttcgccactt cttcgtgacc ttcggttgga
     6541 acgacctggg aatcaagcgc cgcttcctca ccgtgatctg cgcgggtatc accgcgatca
     6601 tgctggccgt ggcgctcaca cccctgtccg agctcatctg gggcaagctg atgggcatcc
     6661 cggaaccggt ccgcgacata gccgagcatg tgcttgtggt gatgtgcctg atgccagctc
     6721 tgatcgtgtt ccggaactac tatcacggcc ggctgatggt cgagcgccgg acgtcgggca
     6781 tggccgctgg cggcattctc agagtcatcg cgatacttct tgcggcgcag gcatgcacga
     6841 tgatgggctg gctgaactac ctgagcgcgt cgttcatcct gattctgggc tttctggtgg
     6901 aagcgctggt ggcgctcgta gcggctcaga aaggccaggg actgcgggca aatgccaccg
     6961 cgaatatgta gcctacgctt gccagggcac cagcgaaccc agcgagcttc agcggcgtcg
     7021 agctcgcccg catcgtgagg actccgaatc cgatgtaggc caggagcccg gcgagctttg
     7081 ctgccagcca gccttccagt ggcgaaacgc tgagctgcac ggccagcgcc acaccgagca
     7141 cgagcagcgc cgtatcgatg acgtgcggcg agatacgcac ccacttctgg ttcttcagcg
     7201 gagagtcgac cagggaccag attcccctga ccacaaaccc gagcaccgtc aggtaggcaa
     7261 gaatcagatg gatcgatttc agcattgtct ctccccgggc ggtcctcgat cacttcgttt
     7321 atgatcggcc tcccagcgca ttcaagcaac ccgacacctc ggaaaactat ctatgaatat
     7381 tgatcaggcc atcgaccagg ccatgcggcc ccttgcggac gcggtgtccg gattcatctt
     7441 cttctctgtg cccgttctcg gaacccagat cccgctgatc gtggtctggc ttatcctcgg
     7501 ggggcttttc ttcacagtgt atctgcgctt cgtcaacatt cgcggcttcg cccacgccat
     7561 cgggctggtc agcgggcgct accacaacga ccaacacccg ggtgagatct cgcagttcca
     7621 ggctttgacg actgcggtat cgggcacggt gggaataggc aacatcgccg gggtagccgt
     7681 ggccatttcc atgggcgggc ccggcgcgac gctgtggctg atcatcgccg gcctgctggg
     7741 catgtcgacg aagttcgccg aatgcaccct cgggctcaag tttcggcggc atcacgagga
     7801 cggctcggtt tccggaggcc cgatgtatta tctcgagcgc ggcctggcgg agcgcaactg
     7861 gccgcgggtc gggcgcgggc tcgggctgtt ctacgcggca tccatggtca tcggctgtct
     7921 cggtatcggc aacatgtttc agtcgaacca ggcggcagcc atcttcatag acgtcagcgg
     7981 cggcgatgcg agctacttcc aggacaaggc ttggctactg ggcctgatca tggccggcgc
     8041 ggtagggctg gtaatcatcg gcggcatccg ttccattgcg cgcaccacct cgaggctggt
     8101 ccccggtatg gcgctgctgt acgtgatctc ggcgcttgcc atcatcgcca tcaacgcggc
     8161 cgaactgcct gccgccttcg ccgccatctg ggcgggcgcc ttctctccgg agggtatagc
     8221 cggaggcctc atcggcgtca tgatcatcgg tttccgcaga gccgtgttct ccaacgaggc
     8281 cgggctcggc tccgctgcca tcgcccactc cacggcgaag accgaacgcc cggtgagcga
     8341 aggctacgtc gctctcctgg agcccttcat cgacaccgtc gtgatctgca ccatgaccgc
     8401 cctggtcatc atcacgacgg tgtacgaacc cgggctgacc agcggcaacg tgagcgggat
     8461 cgagctcacc acccgcgcgt ttgcgagcac gctgtcatgg tcgccgatac cactgtccat
     8521 cgccgccatc ctcttcgcgt tctccaccat gattgcctgg tcctactacg gcctgaaggc
     8581 cttcacctat ctggtgggcc accaccgggt cgccgacctg ggcttcaaga tcttcttcct
     8641 cgtcttcgtg gttctgggca gcagcgttca gctgggtgcc ctggtggacc tctccgacgc
     8701 gctcgtgttc gtggtcgcga tccccaacct gctggggctg tacctgcttg cccccacagt
     8761 gcgggccgag ctgagggcct atgaatccaa catgccggtg cccaacggcg cggcgccggc
     8821 tcccgacagc acctgagccg ggcgcgttat aatgcgcgag ctctcagcac gccggaacgc
     8881 tttatcgaca atggaaggac ccacctccca cgtttacttc tcccagcggc tcaggctgca
     8941 ttacgtggat tggggcaacc ccgacgctcc acccatgctg atgattcacg gcggccgcga
     9001 tcactgcagg aactgggatt gggtggcccg gtcacttcgc gaccgctacc acatcatcgc
     9061 ccccgacctg cgcggtcacg gcgactctca gtggatgctc ggcgggagct acaaccagat
     9121 cgactacgtg tacgacatcg cccagctgct gactcagaag gacatgacgc cggtgacgat
     9181 catcgggcac tcgctgggtg gctccatcgc gctcacttac tcgggtctgt tcccggagac
     9241 cgtgaagaag ctggtagcca tcgagggcat ggggccgccg cccgagttca tcaaggaacg
     9301 g
//
LOCUS       DBNHOX010000603         2681 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1269213, whole genome shotgun sequence.
ACCESSION   DBNHOX010000603 DBNHOX010000000
VERSION     DBNHOX010000603.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1269213"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="AC16UZ_13230"
     CDS             complement(<1..393)
                     /locus_tag="AC16UZ_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6247692.1"
                     /translation="MTNQPVIRTEGVSKSVPTSEGELSILESIDLEINAGETVAIVGA
                     SGSGKTTLLGILAGLDLPTSGRVYLVGEELTALDEEQRARVRGEQVGFVFQTFQLLRS
                     LTALENVMLPGELRGDGDAQPEALRVLDK"
     gene            413..1003
                     /locus_tag="AC16UZ_13235"
     CDS             413..1003
                     /locus_tag="AC16UZ_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HIR6247693.1"
                     /translation="MIALIAGVSPASARSKILVMGDSISAAYGIQRDAGWVALLDRRL
                     SETASPWQVVNASISGETTGGGLARLPKALEAHDPEIVIIELGGNDGLRGYPVSRIRE
                     NLDKLVELAVTGGRRVLLLGMRIPPNYGPRYTRAFEDMYRDVAEAHGVRFVPFILEAV
                     ALNPGLMQDDGIHPTAEAQQKLLDSVWPELQQVLDG"
     gene            complement(1000..1959)
                     /gene="trhO"
                     /locus_tag="AC16UZ_13240"
                     /gene_synonym="yceA"
     CDS             complement(1000..1959)
                     /gene="trhO"
                     /locus_tag="AC16UZ_13240"
                     /gene_synonym="yceA"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent tRNA uridine(34) hydroxylase
                     TrhO"
                     /protein_id="HIR6247694.1"
                     /translation="MRGRRAGVTSVVTFYKFVRLLDPSGLRDELDGMACELALKGTIL
                     LAGEGINGTVTGNAVALQKFLGALERLPEFSGLAVKYSSAAAGNPVFHRLKVRVKPEI
                     VSFGVSGLDPGGRTGTHVGAEEWNELLDDPEVTVVDTRNDYEVAIGSFPGAVNPATQS
                     FRQFADFVADHLDPDRDTRVAMFCTGGIRCEKASAYLLERGFAEVYQLDGGILNYLET
                     VKGEANRWEGECFVFDQRVSVDRHLREGSYEQCFACRRPLSTDDLQSPDYRPGISCPH
                     CAQEQPEARRAALAERARQVALAARRGEAHIGVRQGSQGRTNG"
     gene            complement(1935..2249)
                     /locus_tag="AC16UZ_13245"
     CDS             complement(1935..2249)
                     /locus_tag="AC16UZ_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HIR6247695.1"
                     /translation="MSIQDRINERLQSRFDLHHLEVVNESGGHNVPPGSETHFKVILV
                     SPQFEGLRLIQRHRLVHETLAEELACGVHALAVHTYTDAEWRSRFGSAPMSPPCEGGG
                     RA"
     gene            complement(2269..>2681)
                     /locus_tag="AC16UZ_13250"
     CDS             complement(2269..>2681)
                     /locus_tag="AC16UZ_13250"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HIR6247696.1"
                     /translation="RYLLGHVGDSRAYRLSGAVLEPLTRDHSVVQAMVDDGRLSEADA
                     LCAPDRNLITRAVGLEVDVFADVVELEYEAEDAFLLCSDGLTDLLAPDELAEICDGHL
                     GPAMRDLPDSLVNAANAAGGYDNITVVLVYNCDP"
BASE COUNT          479 a          890 c          868 g          444 t
ORIGIN      
        1 cttgtccagc acccgcagag cttcgggctg cgcatcccca tcgccgcgca gctcgccggg
       61 aagcatgacg ttctccaacg cggtcaggct gcgcagaagc tgaaaagtct gaaagacgaa
      121 cccgacctgc tcgccgcgaa cccgcgccct ctgctcctcg tcaagggccg tcagctcctc
      181 accgacgaga tagacccggc cgctggtcgg caggtcgagc ccggcgagaa tacccagaag
      241 cgtggtcttg cccgagccgg aggcgccaac gatggcgacg gtttctcccg cgttgatttc
      301 gaggtcgatg ctctccaaga tggagagttc cccctcactc gtcggcacgc ttttggacac
      361 cccttcggta cgaattaccg gctggttcgt caaatcggta ttcctgaccc tgatgattgc
      421 gctgatcgcg ggcgtctcgc cggccagcgc gcggtcgaaa attctggtga tgggcgacag
      481 tatcagcgct gcgtacggta ttcaacgcga cgccggctgg gtcgccctgc tggaccggcg
      541 actcagcgaa accgcaagcc cgtggcaggt ggtcaacgcc agcatcagcg gcgaaaccac
      601 gggcggaggg ctggctcgcc tgcccaaggc cctcgaagct cacgatcccg agatcgtcat
      661 catcgagctg ggcggcaacg atggtctgcg cggctacccg gtgagccgca tacgggaaaa
      721 tctcgacaag ctcgtggagc tggccgtgac gggcgggcgc agggtgctcc tgctcggcat
      781 gaggatcccg cccaactacg ggccccgcta tacgcgcgct ttcgaggaca tgtatcgcga
      841 cgtcgccgag gcccacggcg tgcgtttcgt gcctttcatc ctggaggcgg tggccttgaa
      901 cccgggcctg atgcaggacg acgggatcca tccaacggct gaggcgcaac aaaagctgct
      961 ggattccgtc tggcccgagc tgcagcaggt gctggacggc tagccgttcg ttctcccctg
     1021 gctcccctgg cggacgccga tgtgcgcctc accacggcga gcggccagcg ccacctgccg
     1081 ggcccgctcg gcgagcgcgg ctcgccgggc ctcgggctgc tcctgggcgc aatgggggca
     1141 ggaaatgccg gggcggtagt cgggcgattg caggtcatcg gtcgacaacg gccgccgaca
     1201 ggcaaagcac tgctcgtacg agccctcccg aagatgccgg tcgacggaga cgcgctggtc
     1261 gaacacaaag cattcgcctt cccaacgatt cgcctcccct tttaccgtct ccaggtaatt
     1321 gaggatgccg ccgtcgagct gatatacctc ggcgaagcca cgttcgagca gataggcaga
     1381 ggccttctcg caacgaatgc caccggtgca gaacatggca accctggtgt cgcggtcggg
     1441 gtcgaggtgg tcggcgacga agtccgcaaa ctggcggaag ctctgcgtcg ccgggttgac
     1501 ggcaccggga aaggagccga tcgcaacttc gtagtcgttg cgcgtgtcga cgacggtcac
     1561 ctcggggtcg tcgagcagtt cgttccattc ctcggcaccc acatgggtgc cggtccggcc
     1621 acccgggtcg aggccagaaa cgccgaagct gacgatctcc ggcttgacgc gaaccttgag
     1681 ccggtggaac accggattgc ccgcagccgc ggaagaatac ttgaccgcca ggccggagaa
     1741 ttccggcaac cgctcgaggg cgcccagaaa tttctgcaga gccacggcat ttcccgtgac
     1801 cgttccgttg atgccttccc cggcgagcag gatcgttccc ttcagcgcca gctcgcaagc
     1861 catgccatcg agctcgtcgc gcagccccga gggatcgagg aggcgcacga acttgtagaa
     1921 cgtgacgacg ctggtcacgc ccgcccgccg ccctcgcatg gcggcgacat gggcgcgctg
     1981 ccgaagcgcg agcgccactc ggcgtcggtg taggtgtgaa cggccagcgc gtgcacgccg
     2041 caggccaact cctcggcgag cgtctcgtgg accagccggt ggcgctgtat caggcgcagg
     2101 ccttcgaact gcggggagac gagaatcacc ttgaagtggg tctccgaccc gggcggcacg
     2161 ttgtgcccgc cgctttcgtt gaccacctcg aggtggtgca gatcaaagcg ggattgaaga
     2221 cgctcgttta tccggtcttg gatcgacatc gacagcagtt gcaaaccatc aggggtcaca
     2281 attatagacg agcaccacgg tgatgttgtc gtatcccccc gccgcgttcg cggcattcac
     2341 gaggctgtcc ggaagatccc gcatggccgg ccctagatgg ccgtcgcaga tttccgccag
     2401 ctcgtccgga gcgagcagat cggtgaggcc atcggagcag agcagaaacg catcttccgc
     2461 ttcgtactcg agctccacca cgtccgcgaa cacatccacc tcgagcccga ccgcccgggt
     2521 gatcagattg cgatcgggcg cgcacagggc gtccgcttcg ctcagccggc catcatccac
     2581 catggcctgc acgacgctgt gatcgcgggt aagcggctcg aggacggcgc cgctcagccg
     2641 gtacgcgcgc gagtcgccca cgtggccgag caggtagcgg c
//
LOCUS       DBNHOX010000604         2595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_370824, whole genome shotgun sequence.
ACCESSION   DBNHOX010000604 DBNHOX010000000
VERSION     DBNHOX010000604.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_370824"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="AC16UZ_13255"
     CDS             complement(<1..572)
                     /locus_tag="AC16UZ_13255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6247697.1"
                     /translation="MTETYLTDERRMIQEVARDFTRREVLPVANELDPIQGDIPMDLR
                     DKLGEMGYFGIRMAEEHGGLGLGVFEYCLISEELARGWMSVASIIARATSLMNVGGWP
                     LEKREELTQKAARGEYLAAISLSEPDVGSDLAAAKCRAVLDGDEWVITGSKYWCTFAD
                     GADYIQLLARVEAPDSDDAQHKGLKNFIIE"
     gene            complement(576..1487)
                     /locus_tag="AC16UZ_13260"
     CDS             complement(576..1487)
                     /locus_tag="AC16UZ_13260"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR6247698.1"
                     /translation="MNRQPKRARRCQLSVPGSSEKMMAKAAALDLDHVFLDLEDAVAP
                     SAKPAARGMIVEALNGLDWTPRTLCVRINDVETQWCHDDIIEVVTGAGEKLDTIMLTK
                     AKSAADVMFVHLMLDQLEQKLGLTRRIGIECLIEEVEGMMHVEEIAACSDRLECLVFG
                     MGDYSASQQMQLGHIGSSGGYPPDVWHYPRYKMTIACHANGIDPVDGPYANFRNSEAY
                     GKEAERAFVLGMVGKWAIHPSQIELANAVFTPDPAAIADARKQKAAYNEALSQGLGAI
                     NVDGVMVDAASIRIVQNLLDRAELIGM"
     gene            complement(1484..1987)
                     /locus_tag="AC16UZ_13265"
     CDS             complement(1484..1987)
                     /locus_tag="AC16UZ_13265"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039588.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6247699.1"
                     /translation="MALTGERNYFEDFQAGQVFRHARGKTMCEQDNVGITLQVLNTAE
                     GHFNEDVMQRNALALGDGRGGWNSRLQFGGVTIAMVIGLAMQDTGENALRELKLDKIR
                     LKAPVFHGDTLYAYTEVLETAEADREDAGIVTFRHWGTNQDDKVVFEGERTVMLKRKS
                     HYGAIPQ"
     gene            complement(1990..2484)
                     /locus_tag="AC16UZ_13270"
     CDS             complement(1990..2484)
                     /locus_tag="AC16UZ_13270"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039588.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6247700.1"
                     /translation="MSETNDFRDRAVLLSKGNRYEDMTEGRVFDHHWGRTITDGDNAA
                     FTTQTLSFCPLYFNEPYARSLGHPGTVVNPLLVFNTVFGLTVEDLSEGGGPFLGVDDC
                     EFLADVYVGDTLTARSTVVTARDSKGQPAFGIVTWHTEGFNQHGEKVLAYHRTNLVRK
                     RKRD"
BASE COUNT          407 a          855 c          826 g          507 t
ORIGIN      
        1 ttctcgatga tgaagttctt cagccccttg tgctgggcat cgtccgagtc gggggcttcg
       61 acccgggcga gaagctggat gtagtccgca ccgtcggcga acgtgcacca gtacttgctg
      121 ccggtgatga cccactcgtc cccatccagc acggcccggc acttggccgc agccagatcg
      181 gagccgacgt ccggctccga aagggagatc gcggccagat actcgccccg cgcggccttc
      241 tgcgtcagct cctcgcgctt ctcaaggggc cagccgccca cgttcatgag cgacgtggcc
      301 cgggcgatga tgctcgccac gctcatccag ccccgcgcca gctcctcgct gatcaggcag
      361 tactcgaaca cgccgagccc caggccgccg tgctcctcgg ccatgcggat gccgaaatag
      421 cccatctcgc cgagcttgtc gcgcaggtcc atggggatgt ccccctggat ggggtcgagc
      481 tcgttggcca cgggcagcac ttctcgccgg gtgaagtccc gggcgacctc ctgaatcatg
      541 cgtcgctcgt cggtgaggta agtctcggtc attggctaca tcccaatcag ttctgcccga
      601 tcgagcaggt tctgcacgat tcggatactg gcggcgtcca ccatcacgcc gtccacgttg
      661 atggctccca gcccctggct caaggcctcg ttgtaagccg ctttctgttt gcgggcgtcg
      721 gcgatggctg ccgggtccgg tgtgaaaacc gcgttggcga gctcgatctg gctcggatgg
      781 atggcccact tgccgaccat gccgagcacg aaagcgcgct cggcctcctt gccgtaggcc
      841 tcggagttgc gaaagttggc gtaagggccg tccaccggat cgatgccgtt ggcgtgacag
      901 gcgatggtca tcttgtagcg cgggtagtgc cagacgtctg gcgggtagcc tcccgagctg
      961 ccgatgtggc cgagctgcat ctgctggctg gcggagtagt cgcccatgcc gaacacgagg
     1021 cactcgagcc gatcggagca ggcggcaatc tcctccacgt gcatcatccc ctccacctct
     1081 tcgatgaggc actcgatgcc gatgcggcgc gtaagcccca gcttctgctc cagctggtcg
     1141 agcatcaggt ggacgaacat gacgtccgcc gcgctcttgg ccttggtcag catgatcgtg
     1201 tcgagctttt ccccggctcc ggtgaccacc tcgatgatgt cgtcgtggca ccactgggtc
     1261 tccacgtcgt tgatgcgcac gcagagggtg cgcggcgtcc agtcgagccc gttcagtgcc
     1321 tccacgatca tgccccgggc tgcgggtttg gcgctggggg ccaccgcgtc ctcgaggtcg
     1381 aggaacacgt gatccagatc cagggccgcg gccttggcca tcatcttctc ggagctgccg
     1441 ggtacggaga gctggcagcg cctggcgcgt ttgggttgtc ggttcattgc ggtatcgctc
     1501 cgtagtggct cttgcgtttg agcatgacgg tacgttctcc ctcgaaaacg actttgtcgt
     1561 cctggttggt tccccagtgc ctgaaggtca cgatgccggc atcctcccgg tcggcctccg
     1621 cggtttcgag cacctcggtg taggcgtaca gcgtgtcgcc atgaaaaacg ggcgccttca
     1681 ggcggatctt gtccagcttc agctcccgca aggcgttctc gcccgtatcc tgcatggcga
     1741 ggccaatcac catggcgatg gtcacgccac cgaactgcag gcgcgagttc cagccgccgc
     1801 ggccgtcccc cagagccagc gcgttgcgct gcatgacgtc ctcgttgaag tgcccctcgg
     1861 cggtgttgag cacctgcagg gtgatcccga cgttgtcctg ctcgcacatg gtcttgcccc
     1921 gggcgtgccg gaaaacctgg ccggcctgga agtcctcgaa atagttgcgc tcgccggtca
     1981 acgccatggt caatcccgct tgcgcttgcg gaccaggttg gtccggtgat aggccagcac
     2041 tttctccccg tgctggttga agccttcggt gtgccaggtg acgatgccga aagccggttg
     2101 gcccttcgaa tcgcgcgcgg tcaccaccgt gctgcgcgcg gtgagcgtgt cgcccacgta
     2161 gacgtccgcc agaaattcgc agtcgtccac gcccaggaac ggaccgcccc cctcgctcaa
     2221 atcctccacc gtgagaccga aaacggtgtt gaagaccagc aggggattga ccacggtccc
     2281 cgggtggccg agggatcggg catagggctc gttgaaatag agcgggcaga aggaaagggt
     2341 ctgggtggta aacgccgcgt tgtccccatc ggtgatcgtg cgcccccagt ggtggtcgaa
     2401 cacccggccc tcggtcatgt cctcgtagcg gttacctttg ctaagcagca cggcccgatc
     2461 gcggaagtcg ttcgtttcgc tcatcgatga acccgcagca ggctgaagaa gtcgcccaga
     2521 tcgtcctggg agtccagggt cctgaccagg ccggcgagct cgtccgcatg ggcacggccg
     2581 acgcggtcgt ccacc
//
LOCUS       DBNHOX010000605         2933 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_113741, whole genome shotgun sequence.
ACCESSION   DBNHOX010000605 DBNHOX010000000
VERSION     DBNHOX010000605.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_113741"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /locus_tag="AC16UZ_13275"
     CDS             <1..1442
                     /locus_tag="AC16UZ_13275"
                     /inference="COORDINATES: protein motif:HMM:NF020885.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tip fiber protein"
                     /protein_id="HIR6247701.1"
                     /translation="AISGLDTRVTTAEGTISAHSTDITNLQSSVTTANGNITNNANAI
                     SALDTRVTSAEGTLTSQASQITALQSEVDLRGYVFAQPAQPTAYNVGDLWIDTDSSPA
                     NQMYRWNGTAWVAVRDAGIAGNASAITAIDTRVTSAEGTISSHASSITSLNSSVTTLN
                     GQVSTAQVDIAANTSAIVANDGEISTLFAKYGVTLNVNGYITGFSQHNNGTSGSFVIL
                     ADKFAIVDPSGDPAEPQFVPFSVSGGVVYMQNVVIGDALISNLTVSKLTSGSFANGQT
                     FTHNGDLNVGTTGRVRGGMTSYATGTGFFLGYEGGAYKFSVGDSSKYIRWDGSTFSVG
                     GDIIATGNIIDGAVSSQDYQVTTTLQSQTENVETTVATATVTTVAGKPVALSSFWNFT
                     TFAPTPYNSAPITTFKLKRGATVLLTWTYTGVYATYDVSRFWIDNPGAGTFTYTITAT
                     QRADPNGDNGTPQTRDVHYIALEARQLKR"
     gene            1446..1874
                     /locus_tag="AC16UZ_13280"
     CDS             1446..1874
                     /locus_tag="AC16UZ_13280"
                     /inference="COORDINATES: protein motif:HMM:NF025581.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HIR6247702.1"
                     /translation="MLVALTLAVALGAADCSDYGTVDNAARAALKEAQSLDQGVEYGG
                     VVFEYKGKVCRTSAAAGEAEEITYEVWLPPGGRVVGLWHTHPPQQGGFSLRDVYASCV
                     RGIPSYVFSPGAGIRRFVPNARACRKSSMTGQRGTFLKEE"
     gene            1878..2063
                     /locus_tag="AC16UZ_13285"
     CDS             1878..2063
                     /locus_tag="AC16UZ_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247703.1"
                     /translation="MSAKQNLESIQKQLQEALAQRALIDEKILSLRNVMQGAGLGVEL
                     EKEAQAESAKAAASNAE"
     gene            2082..>2933
                     /locus_tag="AC16UZ_13290"
     CDS             2082..>2933
                     /locus_tag="AC16UZ_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247704.1"
                     /translation="MPITNASLAQQVSDLLTAWRNREYEFQAWMTGALDGGDYSNGTF
                     PLTDYVGTVRYTKSPARLEADVTGLVDSASAYADAASASAGAASASAATASGFSTSAN
                     SAKTAAESARDLASSYKDMAASSEANALTYSSNALASANAAALSEANASTSETNAAAS
                     EAAALASEIAAAASAAAAATFNPALYAALADNEVITGAWTFSGATTVPVATVTAHQAS
                     LTIAETQITDGSLLARLADNETVTGNWGFKMDTIGDFMFWRNGTTGASGVGFRNDDGV
                     KGYIAFDD"
BASE COUNT          564 a         1013 c          894 g          462 t
ORIGIN      
        1 ccgccatcag cggcctcgat acgcgggtca ccacggcaga gggaacgatc tcggcgcact
       61 cgaccgatat cacgaatctg caaagttcgg tgaccacggc caacggcaac atcacaaaca
      121 acgccaacgc catcagcgcc ctcgataccc gagtcacgtc tgcggaggga acgctcacct
      181 cgcaggcctc tcagatcacc gcgctgcaat ccgaggtgga cttgcggggc tacgtgttcg
      241 cgcagcccgc gcagccgacc gcctacaacg tcggagacct gtggatagac acagactcgt
      301 ccccggcgaa ccagatgtat cgctggaatg gtacggcgtg ggtcgccgtg cgcgacgccg
      361 gcatcgccgg caacgcctca gcgatcaccg ccatcgacac ccgcgtaacg tcagccgagg
      421 ggacgatctc gtcccacgcg tccagcatca cctcgctcaa ctcaagcgtg accacgctca
      481 acgggcaggt gtccacggcg caggtagaca ttgccgcgaa cacttcagcc atcgtggcga
      541 acgacgggga gataagcacc ctgttcgcga agtacggtgt cacgctcaac gtcaacggat
      601 acatcaccgg gttctcgcag cacaacaacg ggacgtccgg cagcttcgtc atcctcgcgg
      661 acaagttcgc gatcgttgac ccctcgggcg acccggcgga accgcagttc gttccgttct
      721 ccgtgagcgg cggcgtggtc tacatgcaga acgtggtgat cggcgacgcg ctgatctcca
      781 acctgactgt gtccaagctg acgtccggct cgttcgcgaa cggccagacc ttcacccaca
      841 acggcgacct caacgtaggc acgaccggcc gggtgcgggg cggcatgacg tcctacgcca
      901 ccggcacggg gttcttcctc ggctacgaag gcggcgccta caagttctcc gtgggcgact
      961 cctcgaagta catccggtgg gacggcagca ccttctcggt gggcggcgac attatcgcca
     1021 ccggcaacat catcgacggt gcggtctcgt cgcaggacta tcaggtcact acgacgcttc
     1081 agagtcagac cgagaacgtc gagaccacgg tggccacggc cacggtcacg acggtcgcgg
     1141 gtaagcccgt ggcgctgtcg tcgttctgga acttcacgac gttcgcgccg acgccgtaca
     1201 actcggcccc tatcaccacc ttcaagttga agcgcggcgc aaccgtgctc ctcacttgga
     1261 cgtacacggg ggtctacgcg acctacgacg tgtcccggtt ctggatcgac aaccccggcg
     1321 ctggcacgtt cacgtacacc atcacggcga cgcaacgcgc cgaccccaac ggtgacaacg
     1381 ggactccgca gacgcgggac gttcactaca tcgcgctcga agcgcgccaa ctcaagaggt
     1441 aaccaatgct cgtagctttg acgcttgcgg tcgcgctcgg tgctgccgac tgttccgatt
     1501 acggaaccgt tgacaacgcc gctcgggccg cattgaagga ggcgcagtca ctcgaccaag
     1561 gtgtcgagta cggcggcgtc gtgttcgagt acaagggtaa ggtatgccgc accagtgcgg
     1621 ccgccgggga agcggaggag atcacctacg aggtgtggct cccgcccggc ggtcgcgtgg
     1681 tgggtctctg gcacacccac ccgccgcagc aagggggctt ctccctgcgg gacgtgtatg
     1741 cgtcctgcgt gaggggcatc ccaagctacg tcttttcccc cggagccggc atccgccgct
     1801 tcgtccccaa cgcccgcgcc tgtcgcaagt cctcgatgac tgggcagcgc ggcactttcc
     1861 tgaaagagga gtgacctatg tccgccaagc agaacctcga atccatccag aagcagttgc
     1921 aggaggccct cgcgcagagg gcgctcattg acgagaagat cctgtcgctg cggaacgtga
     1981 tgcagggcgc cggcctcggc gtcgagcttg agaaggaagc gcaggccgag tccgcgaagg
     2041 cggcggccag caacgccgag taacccccca aggagccatc catgcccatc acgaacgcca
     2101 gcctcgcaca gcaggtctcc gacctcctga ccgcgtggcg taaccgcgag tatgagtttc
     2161 aggcgtggat gaccggcgcc ctcgacggcg gcgactactc caacggcacc ttccccctca
     2221 cggactacgt cggtaccgtg aggtacacga agtcccccgc gcggctcgaa gccgacgtga
     2281 cgggactcgt ggactccgca agcgcctacg cggacgcggc ctcagcttcg gctggcgctg
     2341 cgtctgcgtc ggccgcgacg gcctcgggct tctcgacttc cgccaacagc gcaaagaccg
     2401 ctgcggagtc ggcccgagac ctcgcgtctt cttacaagga catggccgcc tcatccgagg
     2461 cgaacgccct gacctattca tctaacgcgt tggccagcgc caacgccgcc gcgctttccg
     2521 aagcgaacgc gagtacatcc gagaccaacg cagcagccag cgaggctgcg gcgcttgcct
     2581 cggagatcgc tgccgccgcc agcgcggctg ccgccgcgac cttcaacccc gccctgtacg
     2641 cggccctcgc ggacaacgaa gtaatcaccg gggcgtggac tttcagtggc gcgacaaccg
     2701 tgccggtagc cacggtcacg gcgcatcagg cgtccctgac gattgctgaa acccagatca
     2761 ccgacggctc tctgcttgcc cgactcgcgg acaacgagac cgtcaccggg aactggggtt
     2821 tcaagatgga taccatcggg gacttcatgt tctggcgcaa cggcacgacc ggcgcgtccg
     2881 gcgttgggtt ccgcaacgat gacggcgtca agggctacat cgccttcgac gac
//
LOCUS       DBNHOX010000606         1954 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_691967, whole genome shotgun sequence.
ACCESSION   DBNHOX010000606 DBNHOX010000000
VERSION     DBNHOX010000606.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_691967"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="AC16UZ_13295"
     CDS             <1..1358
                     /locus_tag="AC16UZ_13295"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6247705.1"
                     /translation="DNADAEAVVYDAEFREHVAALRPRLPQVRSWIEVASYFEQPADR
                     AAHFAVCYEDVVGNGNDSPLDIERSGNDLLFIYTGGTTGMPKGVMWEHQAIWQAGGSG
                     SSLLNPGQAAPESPREHAQRIANAPLRARMLAACPLMHGTAMLTSINTLSQGGCIVTV
                     PEHSLDPHAMWATVERYRVNSLAIVGDAFAKPLLRALEERPGAYDISSVFGIVSSGVM
                     WSPEVKRGLLRHHPNMVLMDSFGASEAIGFGTSRTTGDEEAATAKFKIGEHCKVFTED
                     HRPVQPGSGEPGFIARSGPIPVGYYKDPEKTAKTFPMIDGVRYSVPGDWCLVEPDGTL
                     TLLGRGSVCINSGGEKIYPEEIEEALKTHPDVEDALVVGVPDERWGQAVTGVIVPMDP
                     ATFDEAALREHVKSQLAPYKAPKRILISEVPLRAPNGKADYKSVTDFARAALGDTADA
                     K"
     gene            complement(1410..>1954)
                     /locus_tag="AC16UZ_13300"
     CDS             complement(1410..>1954)
                     /locus_tag="AC16UZ_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6247706.1"
                     /translation="GAALIREREHGTLEHLLVMPVTPFEIMSAKVLAMAAVVLLASAF
                     ALNVVVRGFMGVPIEGSLPLFFTGTALHLFATTSLGIFLATLARSMPQFGMMLLMVLL
                     PLQMLSGGITPRESMPELVQLVMLAAPNTHFVLLSQGILYRGAGLDVVWPQLMSLAVI
                     GSTLFMFSLARFRKSLASMA"
BASE COUNT          390 a          630 c          626 g          308 t
ORIGIN      
        1 tcgacaacgc ggatgccgaa gccgtggtgt acgacgctga gttccgcgag cacgtggcag
       61 ctctgcgtcc gcgattgcca caggtacggt cctggatcga ggtggcttct tattttgagc
      121 agccggcaga tcgcgccgca cattttgccg tctgctacga agacgtcgtc ggcaatggca
      181 acgactcacc gctcgacatc gagcgcagcg gcaacgacct gctgttcatc tacaccggag
      241 gcaccaccgg catgccgaag ggcgtgatgt gggagcacca agccatctgg caggccggcg
      301 gctccgggtc gtcactgctg aaccctggcc aggcggcacc agagtccccg cgggaacacg
      361 ctcagcgcat cgcgaacgcg cccttgcggg ccaggatgct ggccgcctgt ccgctcatgc
      421 acggcaccgc gatgctgacg agcatcaaca cgctgagtca gggcggctgc atcgtcacag
      481 tgcccgaaca cagtctcgat ccgcacgcca tgtgggcaac ggtcgagcgc taccgcgtca
      541 actcgctcgc catcgtcggt gacgcgttcg ccaagccgct gctgcgggcg ctcgaggaga
      601 gacctggcgc ttacgacatc tccagcgtgt tcggcatcgt cagctcgggc gtcatgtggt
      661 ccccggaagt gaaacgcggc ctgctgcggc accacccgaa catggttctc atggattcct
      721 tcggcgcctc cgaggccatc ggcttcggca cctcccggac caccggtgac gaggaagccg
      781 ccacagccaa gttcaagatc ggcgagcact gcaaggtatt caccgaagat catcgtcccg
      841 tgcagcccgg cagtggtgag ccgggattca tcgcccgctc cggcccgatt ccggtgggct
      901 actacaagga cccggagaaa acggcgaaga cgttccccat gatcgacggc gtgcgctact
      961 cagtccctgg cgactggtgc ctcgtggagc cagacggtac gttgaccctg ctcggccggg
     1021 gctccgtgtg catcaattcc ggcggcgaga agatctatcc cgaggagatc gaggaagcgc
     1081 tcaagaccca tcccgatgtg gaggacgcgt tagtggttgg agtgccggac gagcgctggg
     1141 gacaggcggt caccggcgtg atcgtcccta tggatccggc aacattcgac gaggctgcac
     1201 tgcgtgagca cgtcaagtca cagctcgcac cctacaaggc gcccaagcgg atcctgattt
     1261 cggaagttcc ccttcgggcg ccgaacggca aggccgacta taagagcgtc acggatttcg
     1321 ccagagccgc cttgggcgac accgcggacg caaagtaagc ctggagtccc aaacggtgat
     1381 cggtgtccag ccggtcgccg gctccagtcc tacgccatcg acgccaggga tttgcggaac
     1441 cgcgccagcg agaacatgaa caaggttgac ccgattacgg ccaggctcat cagctgtggc
     1501 cagaccacat ccaggcctgc cccacgatag aggatgccct gagacaacag tacgaagtgg
     1561 gtgttgggtg cagccagcat cacgagctgg accagctcgg gcatgctttc gcgcggcgtg
     1621 atgccgccag agagcatctg caggggcagc agcaccatca gcagcatcat gccgaactgc
     1681 ggcatcgagc gtgccagcgt ggccaggaag atgcccagcg aggtggtggc gaacaggtgc
     1741 agggcagtgc cggtgaagaa aagcggcagc gatccttcga tgggcacgcc cataaatcct
     1801 cgcaccacga cattgagagc aaaggccgag gcgagcagga ccaccgccgc cattgccagc
     1861 accttggcgg acatgatctc gaacggggtg accggcatca ccagcagatg ctccagggta
     1921 ccgtgctcgc gctcccggat cagcgcggcg ccgg
//
LOCUS       DBNHOX010000607         1872 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1143009, whole genome shotgun sequence.
ACCESSION   DBNHOX010000607 DBNHOX010000000
VERSION     DBNHOX010000607.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1143009"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="AC16UZ_13305"
     CDS             complement(<1..704)
                     /locus_tag="AC16UZ_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019485859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6247707.1"
                     /translation="MQQFDMVIVGAGVSGIGAACHFREKCPGKTFAILESRDAIGGTW
                     DLFRYPGIRSDSDMHTFGYNFKPWRNPKAIADGPSIREYVREAAREHDVDPHIRFNHR
                     LRSARWSSDSATWTLEVETPDGVVEFSCNFLFMCGGYYSYAGGYTPDFPGMADFEGPV
                     VHPQQWPDELDYSSKRVLIIGSGATAVTLLPAIAERAAHVTMLQRSPTYMVSRPDRDA
                     IANRLRRIFPEKVAYA"
     gene            complement(815..1702)
                     /locus_tag="AC16UZ_13310"
     CDS             complement(815..1702)
                     /locus_tag="AC16UZ_13310"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HIR6247708.1"
                     /translation="MPRIEDYLTPEQVEACLRRSDWQAARVLGVNYLMIVAAFALSIA
                     WPNPLTIVLSVLILGGRQLGMEVLVHDCAHSVFFARRRVNEIVGHWLCGGLVNTNLYR
                     YRDYHLGHHRYAGTDKDPDLYLAQMYPAPRDSMRRKLLRDVTGQTGIRDTWRQLRVMK
                     PGRNAPFLVLHGMMIAALAAVGALWAYLLWWAAYVFVYPLLTRVRYMGEHGVCFDYAS
                     ADPRENTSSTVASWWERLLVAPNRVNFHLEHHLLASVPLYHLPKMQRWLKESGFYGDK
                     DCFSHGYLDVVRKATVPAA"
BASE COUNT          369 a          610 c          568 g          325 t
ORIGIN      
        1 atcgcatacg ccaccttctc cgggaagatc cggcggagcc ggttggcgat ggcatcccga
       61 tcgggacggg aaaccatgta ggtcggcgat ctctgcagca tggtgacgtg cgctgcccgc
      121 tcggcgatcg ccggcagcag cgtcaccgcc gtggccccgc tgccgattat cagcacgcgc
      181 ttgctcgaat agtcgagctc atccggccac tgctgggggt gaacgacggg accctcgaaa
      241 tcggccatgc ccgggaagtc gggcgtgtag ccgcccgcgt agctgtagta gccgccgcac
      301 atgaagagaa agttgcagga gaactccacc acgccatcgg gggtttccac ctcgagcgtc
      361 caggtagccg agtccgagct ccagcgggcg gagcgcaggc ggtggttgaa gcggatgtga
      421 ggatcgacgt cgtgctcccg cgccgcttcc cgcacgtact ccctgatgga cgggccgtcg
      481 gcgatggcct tcggattgcg ccagggtttg aagttgtacc cgaacgtgtg catgtcgctg
      541 tcggagcgga ttcccggata gcggaacagg tcccaggtac ccccaatggc atcgcgcgac
      601 tcgagaatgg caaacgtctt cccgggacat ttctcccgaa agtggcaggc ggctccgatg
      661 ccggatacac ctgctcccac gatgaccatg tcgaattgct gcatcttgaa gctcacctca
      721 accctgccgg cccgatgccg acgcccacgt caatgggcgg atcacaaagg ggcaggattg
      781 tgccgcttgc aaggctcatt tgcaccccga ccgtttacgc ggcgggcacc gtcgccttcc
      841 tgaccacgtc caggtagccg tgagagaagc agtccttgtc gccgtaaaag ccgctttcct
      901 tcagccaacg ctgcatcttc ggcaggtgat agagcggaac gctggcaagc aggtggtgct
      961 cgagatggaa gttcacgcga ttcggagcga ccagcaggcg ttcccaccag gacgccaccg
     1021 tgctgctcgt gttctcgcgg gggtcggcgc tcgcatagtc aaagcacaca ccgtgctcac
     1081 ccatgtagcg tacgcgcgtc agcagcgggt acacgaagac gtaggccgcc caccagagca
     1141 ggtacgccca caaggccccc acggcggcga gagccgcgat catcatccca tgaagaacca
     1201 ggaacggcgc gttgcgaccc ggcttcatca cccgcagctg ccgccacgtg tcacgaatgc
     1261 ccgtctgccc agtgacgtcg cgaagcagct tgcgacgcat gctgtcgcgc ggggccggat
     1321 acatctgcgc caggtacagg tcgggatcct tgtccgtgcc cgcgtaacgg tgatggccga
     1381 ggtgatagtc ccggtagcga taaaggttgg tattgaccag cccgccgcac agccagtgcc
     1441 ccacgatctc gttgaccctg cgccgcgcga agaacacgct gtgggcgcaa tcgtgcacga
     1501 gcacttccat acccagctgc cgcccgccca gaatcagcac gctcaacacg atggtcagcg
     1561 ggtttggcca ggctatggaa agcgcgaacg cggcgacgat catcagataa ttcacgccaa
     1621 gcacgcgggc agcctgccag tcagatcggc gcaggcacgc ctcgacctgc tcgggcgtca
     1681 gataatcttc gatacgcggc acgtcagcca acgggcacct cctcgccgtt tcaccacggc
     1741 acgacggtat ttattacacg ttttgacctt aatagtcaaa atttgttatt tataagcggc
     1801 atggacgata ccaacctcac cgccaggcag ctgatcctcg ccctgctcga ttcagcgcct
     1861 gcgccgcaga tc
//
LOCUS       DBNHOX010000608         3831 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1078122, whole genome shotgun sequence.
ACCESSION   DBNHOX010000608 DBNHOX010000000
VERSION     DBNHOX010000608.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1078122"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="AC16UZ_13315"
     CDS             <1..1449
                     /locus_tag="AC16UZ_13315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR6247709.1"
                     /translation="THLPGGLPAFTLVGLPETAVRESRDRVKSAIDNCGLSFPNGKVV
                     VNLAPADLAKEGARLDLAIAVSILAATGQLPHRVLEGFEFLGELGLSGELRPIRGCLC
                     AALASATEVHERRLVLPSANAGELAMAPPGRVVPLAHLREVVRLLREPGYRPAAAPSA
                     KPAANAERESRSLAEVIGQPGAKRALAVAAAGGHHLLMTGPPGTGKTMLARRLAGLLP
                     ALTEAEAREAALVYSAAGLPLEYPFVPPFRDPHHTASGPALVGGGGHPQPGEISLAHR
                     GVLFLDELPLFKPSVLEVLREPLETRSITIARARYRARFPASFQLVAAMNPCPAGRVC
                     SESACRCTPEQVRRYRARISGPLLDRIDLHVAVPPIPKALLLAGARDSSSGVDQTKAA
                     IAGARRVQLSRQGRLNVDLTGADATRLTRLNTESRRLLANAVDRFQLSARGFHRVLKV
                     ARTVADIEGHVDVGPPHVAEALSYRSVTWEGE"
     gene            complement(1454..1912)
                     /locus_tag="AC16UZ_13320"
     CDS             complement(1454..1912)
                     /locus_tag="AC16UZ_13320"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6247710.1"
                     /translation="MNFLLIGLALSGVALVAHAVPPGSEDEIRARTAPMGALCRAGEN
                     CSGFGAPAAMSAPAVAGAQAPAGAPAAAAQSGEQIYNQFCVACHAVGVANAPKLGDVA
                     AWADRIAKGMDVLMASTLNGINAMPAKGTCMTCTDADLQAAVEYMVDGSR"
     gene            complement(2025..3188)
                     /locus_tag="AC16UZ_13325"
     CDS             complement(2025..3188)
                     /locus_tag="AC16UZ_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009686373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HIR6247711.1"
                     /translation="MSAAEFDFGFDETLNMLRDSVRQFAADEIAPRAAQIDASNEFPR
                     DLWPKLGALGLLGVTVEEAYGGAGLGYLAHCIAMEEISRASASVGLSYGAHSNLCVNQ
                     IRRYATEEQKQRYLPPLISGDYLGALAMSEPGAGSDVVSMRLRAEDRGDSFVLNGSKM
                     WITNGPGADVLVVYATVDPSLGPRGITAFLIDKGTPGFSTAQKLDKLGMRGSDTCELV
                     FDDCEVSKTQVLGEVGQGVRILMSGLDYERVVLAAGPLGIMQSCLDTVLPYVHEREQF
                     GQPVGTFQLMQGKLADMYTTMNACRAYVYAVAQACDAGRTTRFDAAGCILFAAEKATW
                     MASEAVQALGGNGYINDYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFDATG"
     gene            complement(3211..>3831)
                     /locus_tag="AC16UZ_13330"
     CDS             complement(3211..>3831)
                     /locus_tag="AC16UZ_13330"
                     /inference="COORDINATES: protein motif:HMM:NF044986.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HIR6247712.1"
                     /translation="IAEEHADELLAEEDHLPAICAAIAAGRLGDSVGPWARRDGFRLN
                     GPASFIQRFRRERKEVEVVLSEGQAQVDGEVFDVADVSWNEGVLALRVDGNRVGARVV
                     TAGPSLYVIIGGSTARFDPGDVEGAARSLGSAAGNRVVSPMPGQVIAVSVKPGDRVKA
                     GAVLAVVEAMKMEHSVAAPRAGRVASVNCRVGDRVDEGVELVVLEE"
BASE COUNT          655 a         1351 c         1211 g          614 t
ORIGIN      
        1 acacatcttc ccggcggcct ccccgcattc accctggtcg ggctccccga aaccgcggtc
       61 agagaatccc gcgaccgggt caagagcgcc attgacaact gcgggctgtc gtttcccaac
      121 ggaaaggtgg tggtgaattt agcgcccgcc gatctggcca aggaaggcgc caggctggat
      181 ctggccatcg cggtcagcat cctggcggcc accggccagc tgccccaccg ggttctggag
      241 ggattcgagt ttctgggcga gctggggctt tccggcgagt tgcgccccat ccggggctgc
      301 ctgtgcgccg cactcgcatc cgcaacggaa gtccacgagc ggcgcctggt gctgccgtcg
      361 gccaatgccg gagagcttgc catggcgccc cccggccggg tggttcctct cgcccaccta
      421 cgcgaggtgg tgcgtctgct gcgggagcca ggctaccggc ccgcggccgc accatccgcc
      481 aagcccgccg cgaacgctga acgggagagc cgctccctgg cagaggtgat cgggcagccc
      541 ggcgcaaagc gagcgctggc cgtcgccgct gccggcgggc atcacctgct catgacgggg
      601 cctccgggaa ccggcaagac gatgctggcg cgcaggctgg ccggcctgct gcccgcgctg
      661 acggaggccg aagcccgcga agccgcgctg gtctactcag cggcgggact acccctggaa
      721 tatccgttcg tacccccatt cagagacccg caccacacgg cttccggccc tgcgctggtc
      781 ggcgggggcg gacaccctca gccgggcgag atctctctgg cacatcgagg cgtgctgttc
      841 ttagacgagc tgcccctgtt caaacccagc gtgctggagg tgctgcgcga gccgctcgag
      901 acccgctcga tcaccatcgc ccgagcccgc taccgggccc gttttccggc gagctttcag
      961 ctcgtcgccg ccatgaatcc ctgcccggcg ggtcgggtct gcagcgaatc cgcctgccgg
     1021 tgcacgccgg agcaggtgcg cagatatcgg gcccgcatct ccggccccct gctggaccgc
     1081 atcgatctcc acgtggcagt gcccccgata ccaaaggcgc tgctgctggc gggagcccga
     1141 gactccagca gcggcgttga tcagacgaag gccgccatcg caggcgcccg ccgcgtccag
     1201 ctgtcgcgcc aggggcggct caacgtggat ctcacggggg ccgacgccac caggctgacc
     1261 agattgaaca cggagagccg acggctgctg gccaatgcgg tggaccgctt tcaactatcc
     1321 gccaggggat tccaccgcgt gctcaaagtg gcgagaaccg tggcggacat cgaggggcat
     1381 gtagacgtgg ggccaccgca tgttgcggaa gcactgagct accggtcggt gacctgggag
     1441 ggtgagtgat cggctaccgg gagccgtcta ccatgtactc cacggccgcc tgcagatcgg
     1501 cgtccgtgca ggtcatgcag gtgcccttgg cgggcatggc gttgatcccg ttgagcgtgc
     1561 tcgccatgag cacgtccatg cctttggcga tgcggtccgc ccacgccgcc acatctccca
     1621 gcttgggggc gttggccaca cccacggcat gacaggctac acagaactgg ttgtagatct
     1681 gctcgccgga ctgagccgca gcggccggcg cccctgcagg ggcttgagcc ccagcgaccg
     1741 ccggggccga cattgccgcc ggcgccccaa agcccgagca gttctcgccg gctcggcaaa
     1801 gtgcccccat cggcgcggtg cgggcacgga tctcgtcctc gctgccgggc gggaccgcat
     1861 gagcgaccaa ggcgacgccg ctcagggcca ggccaatcag taagaaattc atacccttcg
     1921 atctgacaac caggcttcgc aaatctcttt ctccaggtga gatgggcgga aaaaacatgc
     1981 cgagagggca gcgcaagctg ccgggcgcga aattatagcc aaggtcatcc ggtggcgtcg
     2041 aacaactccc gaccgatgag catgcggcga atctcgctgg ttcccgcacc gatctcgtag
     2101 agtttcgcat ccctgagcag ccgcccggtg ggatagtcgt tgatgtagcc gttgccgccc
     2161 agggcctgca ccgcttcgct ggccatccag gtggccttct cggcggcgaa caggatgcag
     2221 ccggccgcgt cgaaacgcgt cgttcgcccg gcatcgcacg cttgcgcaac cgcgtagacg
     2281 tatgcccgac aggcgttcat ggtggtgtac atgtccgcga gctttccctg catgagctga
     2341 aaggtgccta ccggctgtcc gaactgctct cgctcgtgca cgtagggcag cacggtatcg
     2401 aggcagcttt gcatgatgcc cagcggcccg gccgcaagaa ccacccgttc gtagtccagc
     2461 ccgctcatca gaattcgaac cccctggccc acctctccga gcacctgggt cttggacacc
     2521 tcgcaatcat cgaagacgag ctcacaggta tccgatcccc gcatacccag cttgtcgagt
     2581 ttctgagcgg tgctgaaacc cggcgtgccc ttgtcgatga gaaacgccgt gatcccgcga
     2641 ggacccagac tcgggtccac cgtggcgtaa accaccagaa cgtccgcccc cggaccgttg
     2701 gtgatccaca tcttgctgcc attgagcacg aagctgtcgc cccggtcctc ggctcgaagc
     2761 ctcatgctga ccacgtcgga gcccgcgccc ggctcgctca tggccagagc acccaggtaa
     2821 tccccggaaa tcagcggcgg caggtagcgc tgcttctgct cctcggtggc gtagcgacga
     2881 atctgattga cgcaaaggtt cgagtgggcg ccgtaggaca gcccgacgga agccgacgcc
     2941 cgactgatct cttccatggc gatgcagtgc gccaggtagc cgagcccggc gccgccgtag
     3001 gcttcctcca cggtgacacc gagcaggccg agggcgccca gcttgggcca gagatccctg
     3061 ggaaactcgt tgctggcatc gatctgcgcc gcgcggggcg ctatctcatc ggccgcgaac
     3121 tggcgcacgc tgtcacgcag catgttcagg gtctcgtcga acccgaaatc aaattctgca
     3181 gcactcatga cacacggtcc tcccaggaat tcattcctcg agcacgacta gctcgacgcc
     3241 ttcgtccacc cgatcaccaa cgcgacagtt cacggatgcc acccggccgg cacgcggagc
     3301 ggcaacggag tgctccatct tcatggcttc gacgacggcg agaacggcgc ccgccttcac
     3361 ccggtcaccg ggcttcaccg agaccgcgat gacctgcccg ggcatgggcg atacgacccg
     3421 gttacccgca gccgaaccga gggagcgagc cgccccttcg acgtcgcccg gatcgaaccg
     3481 ggccgtcgac ccgccaatga tgacgtaaag gctgggcccg gccgtcacca cccgggctcc
     3541 gacacggttg ccatccaccc gcagcgccag cacgccctcg ttccagctga catcggccac
     3601 atcgaaaact tcgccgtcga cttgggcctg accctcggac agcacgacct ctacctcttt
     3661 tcgttcgcgt ctgaaacgct ggataaagga ggcaggcccg ttcaaccgga acccgtcgcg
     3721 tcgggcccag ggcccgacgc tgtcgccgag ccgcccggcc gcgatggccg cgcagatggc
     3781 gggcaggtga tcttcctcgg cgaggagctc gtccgcatgc tcctcggcga t
//
LOCUS       DBNHOX010000609         2990 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_179101, whole genome shotgun sequence.
ACCESSION   DBNHOX010000609 DBNHOX010000000
VERSION     DBNHOX010000609.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_179101"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2935)
                     /locus_tag="AC16UZ_13335"
     CDS             complement(<1..2935)
                     /locus_tag="AC16UZ_13335"
                     /inference="COORDINATES: protein motif:HMM:NF024141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/M1 family
                     aminopeptidase"
                     /protein_id="HIR6247713.1"
                     /translation="MFLEVFRFESRFQARSPLFLTISTVWFLFGFLITGSESVSVGGV
                     GSNLNLNASFAIIQTITVLTIIGMFAAIAFVASAITRDRETKTAEIFYSSGISERSYL
                     LGRFAGGCVFATAAVTAGVVGTMLGTFMPWLDAERIGSFSLAPYVFSMLVIVLPNMFI
                     ICALFFSVAALTRSMMASYVAALGFLVSFAVIGSATDPENIRYLALGEPFGQVALGEI
                     TRYWTVFDRNFRVPELAGTLLLNRVIWVLVATAVLGITVARFAFELKPARKRWWRRRP
                     NRSEARAAPRHVVDVARASAGMTASLLSQLRMDFRGIIRSVPFYVLLAFGMLNVIGGF
                     YGAVSQWYGTEVYPVTRIMTQVVGSTFAFVVLLILIYYSGELVHRERQSGVADYLDAA
                     PYPNGIMILSKVFGLWLVISLLYLVVVLAGMLVQALNGYREFEPLLYLVSVFGVIGGS
                     FYLWAVPAVLIQSLASNKFLGMAALLVIFLGLTTLEGFGFEHMLYNFGLPAAPYSDMN
                     GYGHFVGRIVSAFAYWASLCVILVVVAHLFYPRGLQTAAERLATARQRFSGGVGAVVV
                     LAALTFCGLGGWIFYNTNVLNEYLTSDDLEEAQADYEKAYRQHRRLPPLQVVDLNLAV
                     DVFPDERRLESRGTARLINANEQPVSDVHLTLPPLLRVNGLSLERAGLVAEDERLGYY
                     QYVLDEALPPGAETTLSWDLSWINEGFPNSGATTRVVGNGTFVDSTEIAPTLGYDSGR
                     ELNDNNVRREYGLPPVERLPKLGDPDWLGTNQLGVSVRSDFRAVVSTSADQIAVAPGY
                     LQREWVEDDRRFFEYAMDQPIWPFVSVSSARYVVARDKWDDVDLEVYHDPKHPYNVET
                     MITATKKSLDYFTREFSPYQYRQVRILEFPAYASFAQSFPNTIPYSEGIGFIADLRDP
                     AEIDYVFYVTAHELAHQWWAHQVVGAQMQGMTVIVETLAQYSALMVMEQEYGPERMRR
                     FLKYEL"
BASE COUNT          631 a          949 c          930 g          480 t
ORIGIN      
        1 ccagctcgta cttgaggaaa cggcgcatgc gctcgggccc atactcctgc tccatcacca
       61 tcagcgccga gtactgagcc agagtctcga cgatgacggt catcccctgc atctgcgccc
      121 cgaccacctg atgcgcccac cactggtggg caagctcgtg cgcggtcacg tagaacacgt
      181 aatcgatttc cgcgggatcg cgaagatcgg cgatgaaccc gatgccctcg gagtagggaa
      241 tcgtgttcgg gaacgactgt gcgaacgacg cgtacgcggg aaactccagg atccggacct
      301 ggcggtactg gtagggggag aattcccggg tgaagtagtc cagcgacttc ttcgtggcgg
      361 tgatcatggt ctcgacgttg tacgggtgct tggggtcgtg gtacacctct aagtccacgt
      421 cgtcccactt gtcgcgggcc acgacgtatc gcgcggacga gacggacacg aagggccaga
      481 tcggctgatc catcgcgtac tcgaagaagc gtcgatcatc ttccacccac tctcgctgga
      541 gatagcccgg ggccacggca atctgatcgg ccgaggtgct gaccacggcg cgaaagtcgc
      601 tgcgaacgct gacgccgagc tggttggtcc ccagccagtc cggatcgccc agcttgggaa
      661 ggcgctccac gggcggcagc ccatactcgc gacggacgtt gttgtcgttt agttcccggc
      721 cgctgtcgta gccgagcgtc ggggctatct cggtgctgtc gacgaaagtg ccgttgccaa
      781 ccacccgagt cgtagcgccc gaattcggaa aaccctcgtt gatccaggac aggtcccagg
      841 aaagcgtcgt ttccgctccc ggcggcaacg cttcatcgag gacgtactgg tagtagccga
      901 gccgctcgtc ttcggcgacg agcccggctc gctctaggga cagcccgttg acccggagga
      961 gcggcggcag cgtaaggtga acgtcgctga ccggctgctc gttggcgttg atcagacgcg
     1021 cggtgcccct cgactccaac cggcgctcat cgggaaacac gtcgaccgct aggttcagat
     1081 ccacgacctg caaaggcggc agccgacggt gctgtcggta agccttttcg taatccgcct
     1141 gggcttcctc caggtcgtcc gaggtcaggt actcgttcaa gacgttggtg ttgtagaaga
     1201 tccaaccccc taggccgcag aaggtgaggg cggcgaggac caccacggcg ccaaccccgc
     1261 ccgagaaacg ctgacgggcc gtcgccagcc tctctgccgc cgtctgcagg ccgcggggat
     1321 agaaaagatg ggcgacgacg acgaggatga cgcacaagct cgcccagtac gcaaacgccg
     1381 aaacgattcg cccgacgaaa tgcccgtagc cgttcatgtc cgaataaggt gcggcgggca
     1441 gcccgaagtt gtagagcatg tgttcgaagc cgaaaccctc cagggttgtc aggcccagaa
     1501 agatcaccag cagcgccgcc atgccgagaa acttgttcga cgccagcgac tggatgagca
     1561 ccgccgggac cgcccacaga tagaacgaac cgccgatcac gccgaacacg ctgacgagat
     1621 agagcaacgg ctcgaactcc cggtagccgt tgagcgcctg gacgagcatg ccggcaagca
     1681 cgaccaccag gtacagcagg ctgatcacca accagagacc gaacaccttg gagaggatca
     1741 tgatgccgtt gggatacggc gcggcgtcga ggtagtcggc caccccgctc tggcgctcgc
     1801 gatgcaccag ctcgccggaa tagtagatga ggatcagcag caccacgaac gcaaaggtgc
     1861 tccccaccac ctgagtcatg atgcgggtga cggggtaaac ctccgttccg taccactggc
     1921 tgacggctcc gtagaaacca ccgatcacgt tcagcatccc gaaggccagc agcacgtaaa
     1981 agggaacact gcggatgatc ccccggaagt ccatgcgcag ctgcgagagg aggctcgcag
     2041 tcatcccggc acttgcccgg gcaacgtcaa ccacgtgacg gggcgcggct cgcgcctcgg
     2101 accggttcgg ccggcgtcgc caccaccgct tgcgggcagg cttcagctcg aaggcaaatc
     2161 gggccaccgt gatccccagg acggccgtcg cgacgaggac ccagatcacg cggttgagca
     2221 gcagcgtccc cgcgagctcc ggaacccgga agttccgatc gaacactgtc cagtatcggg
     2281 tgatttctcc cagcgccacc tgtccgaacg gttctccgag cgccagatac cggatgttct
     2341 caggatcggt ggcgctgccg atcaccgcga aagagaccag aaacccgagc gcggcgacgt
     2401 aggacgccat catcgatcgg gtcagagccg ccacgctgaa gaacaaagcg cagatgatga
     2461 acatgttcgg cagcacgatg accagcatgc tgaacacata aggcgccagg ctgaagctgc
     2521 cgatacgctc cgcatcgagc cagggcatga aggtgcccag catggtcccg acgacgccgg
     2581 cggtcaccgc ggcggtggcg aagacgcacc ccccggcaaa tcgtcccagc agatagctcc
     2641 tctcggatat cccgctgctg tagaagatct cggccgtctt ggtttcgcga tcgcgcgtga
     2701 tggcgctggc cacgaaggct attgccgcaa acatgccgat gatggtgagc accgtgatcg
     2761 tctggatgat ggcaaacgac gcgttcaggt tcaggttgct gccgacaccg ccgacgctca
     2821 ccgattcgct gccggtgatc agaaaaccaa aaaggaacca caccgtggat atcgtcagga
     2881 acagcgggga gcgggcctga aagcggcttt cgaatctgaa gacctcgaga aacatggcgc
     2941 ctcctctcag tcgaccaccg ggttcatgcc gtgcttcatg agggtggcga
//
LOCUS       DBNHOX010000610         7916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_563816, whole genome shotgun sequence.
ACCESSION   DBNHOX010000610 DBNHOX010000000
VERSION     DBNHOX010000610.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_563816"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="AC16UZ_13340"
     CDS             complement(<1..229)
                     /locus_tag="AC16UZ_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR6247714.1"
                     /translation="MSELRTQLVWNRLLAIVEEQAQTLMRTAFSTVVREAGDLSAGVF
                     NTHGDMLAQAVTGTPGHVNAMAASVGKFLKRH"
     gene            complement(238..2340)
                     /locus_tag="AC16UZ_13345"
     CDS             complement(238..2340)
                     /locus_tag="AC16UZ_13345"
                     /inference="COORDINATES: protein motif:HMM:NF014071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR6247715.1"
                     /translation="MSRRDATYRLAVDIGGTFTDVVYEPDAAGAQRVTVKVLTTYDDP
                     ANGVMQGIEAVLERAGAEAGDAGLVLHGTTLATNALIERRGARTALLTTEGHRDALEM
                     ALENRFEQYDVNIDRPRPLVPRRLRLPVRERLDAAGRVLVPMDSKSLDAAVAALDGHG
                     VESVAIGFLHAYANPDHEHAAAERVREKLPDVSITLASQVCPEIREYERLSTACANAY
                     VKPLMARYLGSLQRKLTDRGMVCPVLLMTSGGGLTTLETAAEFPIRLVESGPAGGALL
                     AARIAAEGGHERVLSFDMGGTTAKICLIDGAAPLLSRSFEVDRTYRFKKGSGLPVRIP
                     VIEMVEIGAGGGSVARVDKLSRITVGPDSAGSEPGPACYGRGGRDATVTDGDVVMGRL
                     DPKSFAGGRLALDTAAAERAVTEAIGDPLGLNLHTAAFGISEVVDENMAAAARAHAVE
                     WGEDTQGRTLVAFGGAAPLHAAQLAVKLKLDRVIVPAHAGVGSAIGFLIAPVSYEVVR
                     SRYMRVSGFDADLVNAVMAEMRAEALEVVSSAAETEGLLETRRAYMRYVGQGYEIAVP
                     VPGGTIAEDAGEVLRAAFDHEYRRLYRRTIPNLDVEVLSWSLALAAPQPEFSADSAAP
                     RTDPAEAVRRVSLFEPLTGEMADGAFYRRVDIRPGHWLEGPAVISEEQTTTVVPAGFS
                     AGVTVRGHLVLERMETET"
     gene            complement(2342..3532)
                     /locus_tag="AC16UZ_13350"
     CDS             complement(2342..3532)
                     /locus_tag="AC16UZ_13350"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6247716.1"
                     /translation="MHHYLLGTASWFTAFGIQGVMFAWLVTMVLRESPEMVGLAQMTL
                     LLPGTLLMLIGGTYADRFGGRRIVIGAQSVAACAPLLLLVLIWSDMLTYASMLVYAVF
                     MGCATAFVTPARDGLLNEIAEGRVQRAVMLASMIQFGGQMIGFTLAAGADLVGPLVVL
                     GAQSLCLALGVLAFVLIPRPHEGRHVRTGRLLASLAEGARTVFSSASMRLIVVQNVAM
                     AIFFMGSYVVAMPLLVREVFNGTARDLAFTNGSNSLGLVLSIVLMLRLGDVTRQGRAL
                     VLSQLAGGLMLIAAALMTSFALYVLMLFIWGACGGIAMTMARTIMQEQAPDAQRSRVM
                     SFYALSFMGAGPLGALFTGYMVELLGPQQALVLSGSLMAVVACILAVGSNLWSLRAYQ
                     VASA"
     gene            3769..5301
                     /locus_tag="AC16UZ_13355"
     CDS             3769..5301
                     /locus_tag="AC16UZ_13355"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR6247717.1"
                     /translation="MQDIIEELQARRAAADLGGGEHRIEAQHGKGKLTARERISLLLD
                     EGSFEEWDKFVEHRCTDFSMEEQKIPGDGVVTGYGTVNGRLVFVFSQDFTVFGGALSE
                     AHAEKICKVMDQAMKVGAPLIGLNDSGGARIQEGVASLGGYAEIFQRNVLASGVVPQI
                     SLIMGPCAGGAVYSPAMTDFIFMVKDSSYMFVTGPEVVKTVTHEVVTPEELGGAKVHS
                     GKSGVADLALENDVEALLATRRFVDFLPPNNREKPPRLTTDDPANREEPSLDSLVPAD
                     PAKPYDIKEVIVKVADEADFFEIQPDFAKNIVVGFIRLQGSTVGVVANQPMVLAGCLD
                     IDSSKKGARFVRFCDAFNIPILTLVDVPGFMPGTAQEYGGIIKQGAKLLYAYAECTVP
                     KVTLITRKANGGAYDVMASKHLRGDVNFAWPSAEIAVMGAKGAVEIIFRKDLGDPEKI
                     QARTDEYQQKFANPFVAASRGYVDDVFLPRETRQRICRSFAMLRDKKLENPWRKHGNI
                     PL"
     gene            5321..7306
                     /locus_tag="AC16UZ_13360"
     CDS             5321..7306
                     /locus_tag="AC16UZ_13360"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR6247718.1"
                     /translation="MFNKILIANRGEIACRVIRSARSMGIPTVAVYSDADARALHVTM
                     ADEAVRIGEAPSTESYLRMERIIEACRATGADAVHPGYGFLSENAQFREALDEAGIVF
                     IGPGTRAINAMGDKITSKKLAQEAGVNTIPGYTGVIDGPDHAVEIAREIGYPVMLKAS
                     AGGGGKGMRVVRDDDECRDGFERARNEARSSFGDDRVFAEKFIEDPRHIEIQVMADSH
                     GNVIYLGERECSIQRRHQKVIEEAPSPFLDEATRQAMGEQAVALAKAVDYESAGTVEF
                     IVDAERNFYFLEMNTRLQVEHPVTELVTGQDLVELMIKVAAGEPLPLSQADVTLKGWA
                     IETRVYAEDPFRNFLPSIGRLVHYRQPIETSSVRVDTGIVEGSEVSMFYDPMIAKLIT
                     YGEDRDQAIDRMADALDAYYIRGVSHNISFLNALISHPRFREGRLTTNFIAEEYPDGF
                     HATDLPPTDAMLPIAIVAAVHRAHVERSANISGQTRRFERQIGDDWIVIANGTHYPVN
                     VTAVEHGYEVTYEDSIYRIKTDWRIGDPLVVAEVNGRLVTVQVDRTTAGYRVSQGGTE
                     LDVRVATPTAASLMTHMLEKQAPDLSKFLLSPMPGLLVRLRVAEGEEAKAGEELAVVE
                     AMKMENSLRAEQDVKIAKVLAAEGDSLEVDQPILEFE"
     gene            7441..7611
                     /locus_tag="AC16UZ_13365"
     CDS             7441..7611
                     /locus_tag="AC16UZ_13365"
                     /inference="COORDINATES: protein motif:HMM:NF018058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIR6247719.1"
                     /translation="MSAAIAILLIILILQNMVTVEVRLLWWTVAMPRAILLLLTAMAG
                     FAVGILVRIRRR"
     gene            complement(7625..>7916)
                     /locus_tag="AC16UZ_13370"
     CDS             complement(7625..>7916)
                     /locus_tag="AC16UZ_13370"
                     /inference="COORDINATES: protein motif:HMM:NF016260.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HIR6247720.1"
                     /translation="LAAAAINLGLRSSSSIVADLQRIAGLDELSAVAETQKSFAVVAG
                     KRIGPSLYVRYTYNTLSTIGALIVRYDLTRRWRVEALSSEYSAMDLFYRIGR"
BASE COUNT         1541 a         2634 c         2520 g         1221 t
ORIGIN      
        1 gatgacgctt caggaatttg ccgacggacg cggccatggc gttgacgtga ccgggcgtgc
       61 cggtgaccgc ctgggccagc atgtcgccgt gggtgttgaa caccccggcc gagaggtcgc
      121 cggcctcccg cacgaccgtt gaaaacgcgg tgcgcatcag ggtctgggcc tgctcctcca
      181 cgatggcaag cagccggttc cagaccagct gggtccgcag ttcactcacg ttcgcactca
      241 cgtctcggtc tccatccgtt ccagcaccag gtgacccctg accgtgaccc ctgccgagaa
      301 cccggcgggc accacggtgg tcgtctgctc ttcggagatc acggcgggtc cctccagcca
      361 gtggccgggc cggatgtcga cccgtcgata gaaggcgccg tcggccattt cccccgtcag
      421 cggttcgaac agggagaccc tgcgcacggc ctctgcaggg tccgttcgcg gggccgcgga
      481 gtcggccgaa aactccggct ggggcgcggc gagagcaagc gaccagctca ggacctcgac
      541 atccagattg ggaatggtgc gccggtacag gcgccggtat tcgtggtcga aggccgctcg
      601 gaggacctcg cccgcgtcct cggcgatcgt cccgcccggt acgggcacag cgatctcgta
      661 gccctggccc acgtagcgca tgtaagcgcg acgggtttcc agcagccctt ccgtctctgc
      721 tgcggaggac accacctcca gggcttcggc acgcatctcc gccatgacgg cgttgaccag
      781 gtccgcatcg aaacccgata cgcgcatgta acggctgcgc accacctcgt agctgaccgg
      841 agcgatcagg aaaccgatcg ccgaccccac acccgcgtgg gccggcacga tcacccgatc
      901 cagcttcagc ttgacggcga gctgggcggc atggagcggt gccgctccgc caaaggcgac
      961 aagggtccgt ccctgggtat cttcgcccca ctccaccgca tgggcccggg ccgcagccgc
     1021 catgttctcg tcgaccacct cgctgattcc gaacgcggcc gtgtgcaggt tgagcccgag
     1081 cgggtcgccg atcgcttccg tcacggcgcg ctcggccgcg gcggtatcca gggcgaggcg
     1141 cccgccggcg aaggacttcg gatccagacg gcccatgacg acgtcgccgt cggtgaccgt
     1201 cgcgtcgcgc ccgccacgcc cgtagcaggc gggtccaggc tcggaccccg cgctgtccgg
     1261 gcccaccgtg atgcgcgaca gcttgtctac ccgggcaacc gagccgccgc cggcgccgat
     1321 ctcgaccatc tcgatgacgg gaattcgaac cggcagcccg cttcccttct tgaagcggta
     1381 agtccgatcc acttcgaagg accgggaaag cagcggcgcg gcgccgtcga tcagacagat
     1441 cttggccgtg gttccgccca tatcgaagga cagcacccgt tcatggccac cctcagccgc
     1501 gattcgggcg gcgagcagcg ccccgcctgc gggtcccgac tccaccaggc ggatgggaaa
     1561 ctccgccgcc gtctccaggg tggtgagccc accgcccgac gtcatcagga ggacggggca
     1621 gaccatcccg cgatcagtga gcttacgctg cagactgccc agatagcggg ccatgagcgg
     1681 cttgacgtag gcgttggcgc acgccgtcga caacctctcg tactcgcgga tctcgggaca
     1741 gacctgagag gccagcgtga tcgagacgtc gggcagcttc tcgcgaacgc gctcggccgc
     1801 ggcgtgctcg tgatcagggt tcgcgtaggc gtgcaggaaa ccgatcgcca cgctctcgac
     1861 cccgtgcccg tccagggcgg ccaccgcagc gtccagagac tttgaatcca tcggtaccag
     1921 caccctgcca gcggcgtcca gacgttcccg gacgggcagg cgcaagcgcc ggggaaccag
     1981 gggccgcggc cggtcgatgt tcacgtcgta ctgctcgaaa cgattctcca gcgccatctc
     2041 cagggcatcg cgatgaccct cggtggtcag aagcgccgtt cgcgcgcctc ggcgctcgat
     2101 cagcgcgttg gtcgccagcg tggtgccatg aagaacgagc cccgcgtcgc ccgcctcggc
     2161 gccggcgcgt tccagcacgg cttcaatccc ctgcatcacg ccgttggcag gatcgtcgta
     2221 ggtcgtcagc accttgacgg tgacgcgctg cgcacccgcc gcatcgggct cgtaaaccac
     2281 gtcggtgaac gtaccaccga tatccacggc caaccggtac gttgcgtccc ttctgctcat
     2341 gtcaggcaga cgccacctga taagctctca gagaccagag atttgagccc acggcgagaa
     2401 tgcaggccac cacggccatc aggctgcccg acaggacgag cgcctgctgg gggccgagca
     2461 gctccaccat gtagcccgtg aagagcgcac ccaggggccc ggcccccatg aacgagagcg
     2521 cgtaaaagct catcacccgg ctgcgctggg cgtcaggcgc ctgctcctgc atgatggttc
     2581 ttgccatggt catcgctatt ccgccacagg cgccccagat gaacaacatg agcacgtaaa
     2641 gagcaaagct cgtcatcagc gccgcggcga tcagcatgag gccacctgcc agctgcgaca
     2701 gcaccagcgc ccggccctga cgggtcacat cgccaagccg gagcatgagg acgatgctga
     2761 gcaccagccc gagagagttc gagccgttgg taaaggccag gtcccgggcg gtgccgttga
     2821 acacctcccg gaccagcaac ggcatggcca ccacgtaaga ccccatgaag aagatggcca
     2881 tcgccacgtt ctgcaccacg atcaaccgca tcgacgcaga cgagaagacg gtccgggcac
     2941 cctcggccag ggaagccaac aaccggcccg tgcgcacgtg ccgcccctcg tggggacgcg
     3001 gaatcagcac gaatgccagc acgccgagcg ccaggcagag gctctgagcg ccgagcacca
     3061 ccaggggacc caccaggtcg gcgcccgcag cgagcgtgaa gccgatcatc tgcccgccga
     3121 actggatcat gctggccagc atgaccgcgc gctggaccct gccctccgcg atttcgttga
     3181 gcagaccgtc gcgggccggg gtgacgaaag cggtggcgca acccatgaac accgcgtaga
     3241 ccagcatgct ggcgtaagtg agcatatcgc tccagatcag gacgagcaga agcagcggcg
     3301 cgcaggccgc cacggattgc gcgccgatga cgatgcgccg gcctccgaag cggtccgcat
     3361 aagtgccccc gatcagcatc aggagggtcc ctgggagcag cagggtcatc tgagccagac
     3421 caaccatctc cggagattcg cgcagcacca tcgtgaccag ccacgcgaac atgacgccct
     3481 ggataccgaa cgcggtaaac cagctcgcgg tcccgagcag gtaatgatgc atgggcttca
     3541 agatatcgtt gcggcgaaaa ccgctccgag caggaaacga cgccgcagta taccgcctcg
     3601 aggggctggc cggaccgccc ctgaagcgcc cggcacggcg cgcgcaagat gtggtcaccg
     3661 cgaacgcggc aggatatcct cgcggtttcg tctcggacaa caacaaccat atccctccgg
     3721 gagcatccgc acccggtccg ggggggcatc ccacggcagg agagccagat gcaggacatc
     3781 atcgaggagc tgcaggcgcg acgcgcggca gccgatctcg gcggcggcga gcaccgcatc
     3841 gaagcacagc acggcaaggg caagctcaca gcccgcgaac gcatttccct cctgctcgac
     3901 gagggcagct tcgaggaatg ggacaagttc gtggagcacc gctgcaccga cttctccatg
     3961 gaggagcaga agatccccgg agacggcgtc gtcaccggct acggcacggt gaacgggcgg
     4021 ctggtgttcg tcttcagcca ggacttcaca gtgttcggtg gcgccctgtc cgaagcccat
     4081 gccgagaaga tctgcaaggt gatggaccag gccatgaagg tcggcgcgcc tctgattggc
     4141 ctgaacgatt ccggcggcgc aaggatccag gaaggggttg cgtctcttgg cggctacgcc
     4201 gagatctttc agcgcaacgt gctggcctcg ggcgtggtgc cgcagatctc gctgatcatg
     4261 ggcccctgcg ccggcggcgc cgtctactct ccggccatga ccgacttcat cttcatggtc
     4321 aaggacagct cctacatgtt cgtcaccggt cccgaggtgg tgaagacggt cacccacgag
     4381 gtggtcaccc ctgaggagct cgggggcgcc aaggtgcaca gcggcaaatc gggcgttgcc
     4441 gacctggccc tggagaacga cgtcgaagcg ctgctcgcca cccgccgctt cgtcgacttc
     4501 ctgccgccca acaaccgcga gaaaccacca cggctgacga cggacgatcc cgcaaaccgt
     4561 gaggagccgt ccctcgactc gctggtgccc gcggatccgg caaagccgta cgacatcaag
     4621 gaagtgatcg tgaaggtcgc cgacgaggcg gacttcttcg agattcagcc cgatttcgcc
     4681 aagaacatcg tcgtgggatt catccgtctt cagggatcca ccgtaggcgt cgtagccaat
     4741 cagcccatgg tgctcgccgg ctgcctggac atcgactcct cgaaaaaggg cgcgcgattc
     4801 gtgcgcttct gcgacgcctt caacattccg attctcactc tcgtcgacgt gccgggattc
     4861 atgcccggaa cggcccagga atacggggga atcatcaagc agggagccaa gctgctgtac
     4921 gcgtacgccg agtgcaccgt gccaaaggtc acgctcatca cccgcaaggc taacggcggc
     4981 gcttacgacg tgatggcctc caagcacctg cgcggcgacg tgaacttcgc ctggccgagc
     5041 gccgagatcg cagtgatggg tgcgaaaggc gccgttgaga tcattttccg caaggaccta
     5101 ggagaccccg agaaaatcca ggcccgaacc gacgagtacc agcagaagtt cgccaaccca
     5161 ttcgtggcgg ccagccgcgg ctacgtcgac gacgtctttc tgccccggga gacccgccag
     5221 cgcatctgcc ggtcgttcgc catgctgcgc gacaagaagc tggaaaaccc ctggcgcaag
     5281 cacggcaaca ttcccctgta gagcggaggc ggcgagcagc atgttcaaca agatactcat
     5341 cgcgaaccgc ggggaaatcg catgccgggt gatccgttcc gctcgcagca tggggatacc
     5401 caccgtcgcc gtgtactcgg acgcggacgc tcgggcgctg cacgtcacca tggcagacga
     5461 ggccgtgcgc atcggcgagg caccttccac ggaaagctat ctgcgcatgg agcggatcat
     5521 cgaggcgtgc agggcaaccg gagccgacgc cgttcatccg ggctacggct tcctgtccga
     5581 gaacgcccag ttccgcgaag cgctggatga ggccggcatc gtcttcatcg gtcccggcac
     5641 acgggccatc aacgccatgg gcgacaagat cacgtccaag aagctcgccc aggaagccgg
     5701 cgtgaacacc attcccgggt acacgggcgt gattgacggc ccggaccacg ccgtggagat
     5761 cgcgcgggag atcggctacc cggtcatgct gaaggccagc gccggcggcg ggggcaaggg
     5821 catgcgggtc gttcgagacg acgacgagtg cagggacggg ttcgagaggg cgcgcaacga
     5881 ggcgcgctcg agcttcggcg acgaccgggt gttcgcggag aagttcatcg aggacccgcg
     5941 ccacatcgaa attcaggtca tggctgacag ccacggcaac gtcatctacc tgggagagcg
     6001 cgagtgctcc atccagcgcc gccatcagaa agtcatcgag gaggcgccct ctccgttcct
     6061 cgacgaggca acccgtcagg ccatgggcga gcaggcagtc gctctcgcga aagccgtcga
     6121 ttacgagtcc gccggcaccg tggagttcat cgttgacgcc gagcgcaact tctactttct
     6181 cgagatgaac acccgcctgc aggtggagca tccagtcacc gaactcgtta ccggccagga
     6241 tctggtggaa ctcatgatca aagtggccgc cggcgagccg ctgccgctga gccaggctga
     6301 cgtgacgctc aaaggctggg ccatcgagac gcgggtatac gcggaggacc cgttccgcaa
     6361 tttcctgcct tcgatcggcc gcctggtgca ctatcgccag cccatcgaaa ccagcagcgt
     6421 gcgcgtggat accgggatcg tggaaggcag tgaagtttcg atgttctacg atcccatgat
     6481 cgccaagctg atcacttacg gcgaggatcg tgaccaggcc atcgacagaa tggccgacgc
     6541 gttggacgcc tattacatcc ggggggtctc ccacaacatc agcttcctga acgcgctcat
     6601 cagccacccg cgcttccgcg aggggaggct cactaccaac ttcatcgccg aggagtaccc
     6661 cgacggcttt cacgcaaccg acctgccccc gacggacgcc atgctgccga ttgccatcgt
     6721 tgccgccgtg caccgcgctc acgtggaacg ttccgccaat atcagcggcc agacacggcg
     6781 cttcgaacga cagataggcg acgactggat cgtgattgcc aacggtacgc actatccggt
     6841 gaacgtcacg gcggtggagc acggttacga ggtgacgtac gaagactcga tctatcgcat
     6901 caagacggat tggcgcatcg gagatcccct ggtggtagcc gaggtcaacg gcaggctggt
     6961 cacggtgcag gtggaccgga cgacggctgg ctatcgggtt tcccaggggg gcacggagct
     7021 ggacgtccgc gtggccacgc caaccgcggc aagcctgatg acgcacatgc tcgaaaaaca
     7081 ggcgcctgat ctgagcaagt ttctgctctc tcccatgccg ggtctcctgg tccgactcag
     7141 ggtcgccgag ggcgaagagg caaaggccgg ggaagagctc gcggtggtcg aagccatgaa
     7201 gatggaaaac agcctgcgcg cagagcagga cgtcaagatc gccaaggtgc tcgcggcgga
     7261 gggggacagc ctggaagtcg accaaccgat tctcgagttc gagtaggccg gctcacgccc
     7321 atccgggatt accgaaaacc gtccacagca gatcgcagca ctgcccaatg ttgaagctcg
     7381 cccttagaat gcgccctgac tccaccttcc ggtaagacca tggaaaaatt caggttgggc
     7441 ttgagtgccg ccatagcaat tctgctgatc atcctgattc tgcagaacat ggtgaccgtt
     7501 gaggtccgtc tgctctggtg gacggtggcc atgccaagag ccattctgct gttgctgacc
     7561 gccatggccg ggttcgcggt cggcattctc gttcggatac gcagaagatg agaagctcac
     7621 ggctctagcg cccgatcctg tagaaaagat ccatggcgga gtactcgctg gaaagcgcct
     7681 ccacgcgcca gcgcctggtg agatcgtaac ggacgatgag cgcgccgatc gtcgaaaggg
     7741 tgttgtacgt atagcggacg tacaggctgg gcccgatccg ttttccggca acgacggcaa
     7801 agctcttctg ggtctcggca accgccgaca gctcatcaag gcccgcgatg cgctgcaggt
     7861 cggcgacgat ggacgacgag gaccggagcc cgaggttgat cgctgcggcc gcaagc
//
LOCUS       DBNHOX010000611         7358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_884485, whole genome shotgun sequence.
ACCESSION   DBNHOX010000611 DBNHOX010000000
VERSION     DBNHOX010000611.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_884485"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..590
                     /locus_tag="AC16UZ_13375"
     CDS             129..590
                     /locus_tag="AC16UZ_13375"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR6247721.1"
                     /translation="MIDVLSRWVRLVENGLLVGMLGVMIFLAAYQVIARNFFDSGLIW
                     GDSAVRIAVLWVTLLGAMVASRNDTHIRMDVISQFVDEAARKLLRRIGSVFTCVICGL
                     FAWYSYQFVVLDYQDEIIAFGRVPAWICEAIMPLGGAVMSFRYLLHVFRPA"
     gene            587..1867
                     /locus_tag="AC16UZ_13380"
     CDS             587..1867
                     /locus_tag="AC16UZ_13380"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6247722.1"
                     /translation="MIWVGIVCVLIAAVVGAPLFAVLMAAAMLGFVASDIDLTIVAIE
                     VYRIVDTPLLMVLPLFTYSGYLLAEARTSERLVNLVQSLFGWLPSGLAMVGFVACAVF
                     TALTGASGVTIVALGALLLPALARSGYAEKLGLGLVTASGSLGLLLVPSVPLILYGIV
                     AQQLDVGEPFTIFDLFVAGVLPTLLMLTMLIGWTLWRHRGGVIPRTPFSRQRLWEAVK
                     AARWELPLPVIVLGGIYSGYFAISEAAAVTAVYVTLAEVILYREVTWRELPAVMRNSM
                     VMVGGILLVLGVALAFTNFLVDAEVPQKLFEFMQENIQSKIGFLLLLNLTLLVLGAFL
                     DIFSALVIVVPLILPVAVGYGIHPVHLGIIFLANMQIGYFTPPIGMNLFIASYRFNKP
                     LHELYMASLPFMLVLLVALLLITYVPWFSLVFVE"
     gene            1876..2481
                     /locus_tag="AC16UZ_13385"
     CDS             1876..2481
                     /locus_tag="AC16UZ_13385"
                     /inference="COORDINATES: protein motif:HMM:NF017319.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6247723.1"
                     /translation="MLGILAAAIWLLAAAAHGQAEADTADPGEDPSPRIESFHAALLE
                     AMRSNDAYDVRFASLSPVVGEVFDVPTIARISLGRTWKSLDEPARAQFCELLEELITA
                     TYTDRFDSYSGQTFHLLSAEPARRGWVVKTELEKSDGERVSLDYYFRGSRVFNVVADG
                     VSDLSLRRADYNSIVKQEGYDRLLAHIRDSIAEYEAGGTDG"
     gene            2474..3196
                     /locus_tag="AC16UZ_13390"
     CDS             2474..3196
                     /locus_tag="AC16UZ_13390"
                     /inference="COORDINATES: protein motif:HMM:NF016236.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0120010 - intermembrane phospholipid
                     transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HIR6247724.1"
                     /translation="MAKALMLAVAVLLGGCASLEGPEYGPYDPFDKFNRGSYKFSDAV
                     DKNVLIPVVKGYQAVTPQWLRNGIGNVFSNLRELDSALNGYLQLKPGSGTTDLARVAL
                     NTTVGIGGFFDVAATGGLIYQEEDLGQTLAVAGITRTRYVYVPLAGPTTIRDAPYSFI
                     RAWWPRLLLGSAYGWWMGIIDLLNTRAEVLAVTEARDASALDPYAFTREAYYQRRKFL
                     IYDGDPPMDDFFDDFEDEDEAF"
     gene            3198..5762
                     /locus_tag="AC16UZ_13395"
     CDS             3198..5762
                     /locus_tag="AC16UZ_13395"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR6247725.1"
                     /translation="MAGAWLATPLSRWVGGVSRRPWTVLVCIAVATAALGWVALDRFR
                     MNSNLSDLIRQEATWRVDFDRFKEQFPDLVETAVVVVSGTSYRDVEAMTGRLEAALLG
                     RDDLFRAVYAPENDPFFRRHALLYLDEDDLDDMADRLAEAQPMLTAVAEDPSLRGVLE
                     LVGDGIENEPAAGFDTVVRLLAESGEAVLAGGDPTVSWTDELFETDGTIHRLIFLKGQ
                     ADFQESLANARIMKELRALIAQLPAIDGVDVRITGEVALAHEEIEAAYAGVQIAGWVA
                     LALLMIILIVGVRSAKIIIATVLLLFVGVTWTSAFAMLTVGEYNTLSIVFLVMFFGLG
                     VDFALHFSLRYQEEINSGEGDALRSLVSATNSVGGAIAICTITTALGFLSFYPTPYKG
                     LADLGVISAGGMLVAGFLTFTLLPALYAVLGDIRPHIVDLPTGDRLVAYLSRHRGPVL
                     AALGLGAALALFLASRAHFDYSVLALKDPRAESVVTLRVLQADDAVTDYALTIMSARQ
                     APEAALEELPTVETVLSPADYVPDGQDDKLFVLEDLQELLWSALEPLRTADPPTGTEL
                     REAAEALTATVAAAQAAAPSSPDVDRLAAVLTGLLERPPQALLIWQRGVITNLLEELG
                     WLREALYVDALAFEDLPEKLRARLVSEEGQRVTVVTPSEDVTAVEALSDFVSSVREVA
                     PTATGRPVIEWGVGDIVVGSFKQALGFATFAILAVLFVMFRNLRDSVLILVPLALAAL
                     FTLAASVVMDMPLNMANILVVPLIFGLGVDNGIHVVDRFHRAGDVGHLMHSSTPRAVM
                     LSTLTTIGTFAALMLSPHQGTASIGLLLTLAVGLLLLLTIFVLPVLLSLLVEPAET"
     gene            5759..>7358
                     /locus_tag="AC16UZ_13400"
     CDS             5759..>7358
                     /locus_tag="AC16UZ_13400"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIR6247726.1"
                     /translation="MSGVLLRWACLVVLLGFGVRVAIAVPLWSDPYVPGTEILLAVLV
                     GVYQDLIALVLLGGPLALAFLAGVRVGRAVLLLLVAVVLVVAFAELFFWWEFESRLNR
                     LVFHYLLFPREVVTFLEDQFYVSLYAIPALLIIAAAYLVVRGRSADQRAPSRWFLVGS
                     VLACVVAVLVASPLEVSSSRRINQMASNGYLGVLHAAVTDVSAWHGHYWNPAQPAPTR
                     GEAPGALGVVEPVWQPLPVKHVVLIVEESFSGEVWDDLALRGTYMPRFSSLADSGVLF
                     ENVYSTGTRTTRGLEALLNGFPPLPGIAVNQRGGFERLPSLARALSLAGFHNVFVYGG
                     WPGFSNFFPYWRALGYEELTSRDDFPEPRFETSWGVADEHLFEAIREHMSRLTQNHER
                     VFLTSLTVSHHRPFDFPAGRVPFPAEERRSEYALAYADWALGQFIEDASGEPWFDDTL
                     FVIAADHGPRISGDAPIPVAGFRVPLLLYAPAHLNPRRVDHLGSLMSFGVTLLELLGL
                     PNDEGLYGDNLLLTEQGLVPMEHEY"
BASE COUNT         1063 a         2362 c         2397 g         1536 t
ORIGIN      
        1 tccggtcgcg gtggcccagt ggcaggatct cgccgatgcg gcttctctgg agctggtcga
       61 agccggcatc gtatccgcgc cgatctacga ggacatggtt cgccttctcg tggaataccg
      121 agacagcgtt gattgacgtc ctctcacgct gggtgcggct cgtcgagaac gggctgctgg
      181 tcgggatgct cggcgtgatg atctttctgg cggcgtatca ggtcatcgcc agaaacttct
      241 tcgattccgg tctcatctgg ggcgattccg ccgtgcgcat cgccgtgctc tgggtgacgc
      301 tgctcggcgc gatggtcgct tcgcgcaacg acacccacat tcgcatggac gtcatctcgc
      361 agttcgtaga cgaggcggcg cgaaagctgc tgcggcggat cggcagcgtg ttcacctgcg
      421 tcatctgcgg cctgttcgcc tggtacagct accagttcgt cgtcttggac tatcaggacg
      481 agatcatcgc gttcggccgg gtgccggcat ggatctgtga agccatcatg ccgttgggcg
      541 gcgccgtgat gagcttccgt tatctcctgc acgtgttcag gcccgcatga tctgggtcgg
      601 gatcgtctgc gtgttgatcg ccgccgtggt cggggcgccc ctcttcgccg tgctgatggc
      661 cgccgcgatg cttgggttcg tcgcttcgga catcgacctg acgatcgttg ccatcgaggt
      721 ctaccggatc gtcgacacgc ctctgctgat ggtgctgccc ctgttcacat acagcggcta
      781 cctgctggcg gaggcgcgca cctccgagcg gctcgtgaac ctcgtgcaga gcctctttgg
      841 ttggttgccg agcggtctgg ccatggtggg gttcgtcgcc tgcgcggtgt tcacggcgct
      901 gaccggcgcg tccggcgtga ccatcgtggc gctgggcgcc ctgctcctgc ctgccctggc
      961 gcggtcgggg tatgcggaga agttagggct gggcctggtg accgcttccg gcagcctggg
     1021 gctgctgctc gttccctccg tgccgctcat cctctacggc atcgtggctc aacagctgga
     1081 cgtgggagag ccgttcacga tcttcgacct gttcgttgcc ggcgtgctgc ccacgctgct
     1141 gatgctgacg atgctgatcg gctggacact gtggcgccat cggggagggg tcattccaag
     1201 aacgcccttc tccaggcagc gcctgtggga ggcagtcaaa gcggcgcgtt gggaattgcc
     1261 gctgccggtg atcgtgctgg gcggcatcta ctccgggtac ttcgcgatct ccgaagccgc
     1321 ggcggtaacc gccgtttacg ttacgctcgc cgaggtgatc ctctaccgcg aggtgacctg
     1381 gcgcgagctg cccgccgtca tgcgcaattc catggtgatg gtgggcggga tcctgctggt
     1441 tctgggcgtg gcgctggcgt tcaccaactt tctggtcgat gcggaggtgc cgcagaagct
     1501 gttcgagttc atgcaggaga acatccaaag caagatcggc ttccttctgc tgctcaacct
     1561 gacgctgctg gttctcggcg ccttcctcga catcttctcg gcgctggtca tcgtggtgcc
     1621 gctcattctc ccggtggccg tgggctacgg cattcacccc gttcacctgg gcatcatctt
     1681 tctggcgaac atgcagatcg gctacttcac gccgccaatt ggcatgaatc tgttcattgc
     1741 cagctaccgc ttcaataagc cgctgcacga gctctacatg gcgagcctgc cgttcatgct
     1801 cgtgttactg gtcgctctgt tgctgataac ctatgtgccc tggttcagcc tggtgttcgt
     1861 cgagtgagag agtcaatgct tgggatcctt gcggcggcga tctggctgct cgccgcggcc
     1921 gctcacgggc aagccgaagc agacaccgca gatccgggtg aggacccgtc tcccagaatc
     1981 gaaagcttcc atgcggccct gctcgaggcg atgcgctcca acgacgccta cgatgtgcgc
     2041 tttgcgtcgc tgtcgccggt ggtgggggag gtgttcgatg tccccaccat cgcccgcatc
     2101 agcctcggac gaacctggaa gtccctggac gagcctgccc gtgcgcagtt ctgcgagctg
     2161 ctggaggaac tgatcaccgc gacttacacc gaccggttcg acagctacag cggccagact
     2221 tttcacctct tgagcgcgga gcccgcgcgc cggggctggg tggtcaagac cgaactcgag
     2281 aagtctgacg gcgagcgggt gtctttggat tactacttcc gcggaagtcg ggtattcaac
     2341 gtcgtggcgg acggggtgag cgatctctct ctgcgcaggg ccgattacaa cagcatcgtc
     2401 aagcaggagg gatacgatcg gctgctggcc cacattcgcg atagcattgc cgagtacgaa
     2461 gcgggaggaa cggatggcta aagcgctgat gctcgcggtt gcggtgctgc tgggtggctg
     2521 cgcctcgctg gaggggcccg agtacggccc ctacgacccc tttgataagt tcaaccgcgg
     2581 cagctacaag ttctccgatg cggtcgacaa gaacgtcctc attcccgtgg tcaagggata
     2641 ccaggccgtc accccgcagt ggctcagaaa cgggatcggc aacgtgttct ccaacttgcg
     2701 ggagctggac agcgcgctga acggctatct tcagctcaag cccggatcgg gcaccaccga
     2761 tctggccagg gtggctctga ataccaccgt cggcatcggc gggttcttcg acgtcgccgc
     2821 gacaggcggc ctgatctatc aggaggaaga tctggggcag acgctggccg ttgccggcat
     2881 cacccgaaca cgttacgtct acgtgcctct cgccgggccg accacgattc gggacgcgcc
     2941 ttattccttc atccgggcct ggtggccgcg cctgctgctc ggcagcgcct acggctggtg
     3001 gatgggcatc atcgacctgc tcaatacccg tgcggaggtg ctggccgtga ccgaagcgag
     3061 agacgcctcg gcgctcgatc cctacgcctt tacccgtgaa gcctactatc agcgccgcaa
     3121 gttcctgatt tacgacggcg atccgcccat ggacgacttc ttcgacgact tcgaagacga
     3181 agacgaagcc ttctagaatg gccggcgcct ggttggcaac gccgctgtcg cgctgggtgg
     3241 gcggggtttc gcgccggccc tggacggtgc tggtctgcat cgctgtggct accgccgcgc
     3301 ttggctgggt tgccctcgat cgattccgga tgaactcgaa cctgagcgac cttattcgcc
     3361 aggaagccac gtggcgggtg gatttcgacc gcttcaagga gcagttcccc gacctcgtcg
     3421 aaaccgccgt tgtggtggtt tcgggaacct cgtatcggga cgtcgaggcg atgaccgggc
     3481 ggctggaggc tgcactgctc ggccgggacg atctttttcg cgccgtgtac gcgccggaga
     3541 acgatccgtt cttccgccgc cacgcgctgt tgtacctcga cgaggacgat ctcgacgaca
     3601 tggccgaccg gctggcggag gctcagccga tgctcaccgc cgtggccgag gatccgagcc
     3661 tgcggggcgt gctcgagctc gtcggcgacg ggatcgagaa cgagcccgcg gccgggttcg
     3721 acacggtggt ccgtctgctc gccgaatcgg gcgaggcggt cctcgcggga ggggatccga
     3781 cggtctcctg gacggacgag ctcttcgaga ccgacgggac gatccaccgg ctgatctttc
     3841 tcaaaggtca ggcggatttt caagagtctc tggccaacgc ccgtatcatg aaggagctca
     3901 gagccctcat tgcccagctg ccggccatcg acggggtcga cgtgcggatc accggcgagg
     3961 tggctcttgc tcacgaggag atcgaggccg cctacgccgg cgtccagatc gccgggtggg
     4021 tggcgctggc cctgctgatg atcattctga tcgtcggcgt gcgctcggcg aagatcatca
     4081 ttgccacggt gctcctgctc ttcgtcggcg tgacctggac ctcggcgttc gcaatgctca
     4141 cggtgggcga atacaacacg ctctccatcg tcttcctcgt catgttcttc ggcctgggcg
     4201 tcgacttcgc gctgcatttc tcgctgcgtt accaggaaga aatcaattcg ggagagggcg
     4261 atgccctgcg ctcgctggtg tcggccacga acagcgtcgg tggagccatc gccatctgca
     4321 ccatcaccac cgcgctgggg ttcttgagct tctatcccac gccgtacaag ggcctggccg
     4381 acctgggcgt gatctccgcg ggcggcatgc tcgtggccgg gtttcttacc ttcaccctgc
     4441 tgccggcact ttacgccgtg ctcggggaca tcaggcccca tatcgtcgac ctgcccacgg
     4501 gtgaccgcct ggtcgcctac ctctcgcgac atcgaggacc cgtgctcgcg gcgctcggct
     4561 tgggcgcagc cctcgctctc tttctcgcgt cccgcgcgca cttcgactac agcgttctcg
     4621 cgctgaagga ccccagggcg gagtccgtcg tgacgctgcg cgtcctgcag gcggacgacg
     4681 ctgtgacgga ctacgcgctc accatcatgt ccgcgcggca ggcgcccgag gcggcgctgg
     4741 aagaactgcc gacggtggag accgtgctgt caccggcgga ttacgttccc gacgggcagg
     4801 acgacaagct gttcgtcctg gaggatctgc aggagttgct atggagcgcg ctcgagccgc
     4861 ttagaaccgc cgatccgccc acgggaactg aactgcgtga ggccgcggag gccctgaccg
     4921 cgacggtggc cgcagcccag gccgccgcgc cgtcctcgcc ggacgtggac cggttggccg
     4981 ccgtgctgac cgggctcctc gaacggccgc cccaggcgct gctgatctgg cagcggggcg
     5041 tcattaccaa ccttttggaa gagcttggtt ggcttcgtga agccctgtac gtggatgcgc
     5101 tcgccttcga ggacctgccg gagaagctgc gcgcccgcct ggtcagcgag gagggacagc
     5161 gggtcaccgt ggtgacgcct tcggaggatg tcaccgccgt cgaggcgctg agcgacttcg
     5221 tctccagcgt gcgcgaggtc gcgcctaccg ccaccggccg gcccgtcatc gagtggggcg
     5281 tcggcgacat cgtggtcggc tcgttcaagc aggcgctcgg ctttgccacc ttcgccatcc
     5341 tggccgtgct gttcgtcatg ttccgcaacc tgcgcgacag cgtgctcatc ctcgtgcccc
     5401 tggcgcttgc cgcgctgttt accctggcgg caagcgtcgt gatggacatg cccctgaaca
     5461 tggccaacat tctggtggtg ccgctcatct tcgggcttgg cgtggacaac ggcatacacg
     5521 tggtcgaccg atttcatcgc gcgggcgatg tcggccacct gatgcactcg agcacgcccc
     5581 gggccgtcat gctttcgacg ctgaccacca tcgggacttt cgcggccctc atgctctcgc
     5641 cgcaccaggg aacggcatcc atcgggctgc tgctgaccct cgcggtcggc cttcttctgc
     5701 ttctgaccat attcgttctg ccggtgcttc tgtctctgct cgtcgagccc gccgagacat
     5761 gagtggagtg cttctgcgct gggcctgcct ggtggtcctg ctcggtttcg gcgtcagggt
     5821 ggccattgcg gtcccgctgt ggagcgatcc ctacgtgccg ggcaccgaga tacttctcgc
     5881 cgttctggtc ggcgtttacc aggatctgat cgcgctggtt ctgctcggcg gccctctggc
     5941 gctagccttt ctggcgggcg tccgcgtggg gcgtgcggtt ctcctgctcc tcgtggccgt
     6001 ggtgctcgtg gttgcgtttg ccgagttgtt tttctggtgg gagttcgaaa gccggctcaa
     6061 tcgcctcgtc ttccactatc tgctgtttcc ccgggaggtg gtgacctttc tagaggatca
     6121 gttctacgtg agcctgtacg ccattcctgc gctgctcatc atcgcagcgg cctacctcgt
     6181 cgtgcgcggg cgttcggcgg accagcgcgc gccttcccgc tggtttctcg tcggctcggt
     6241 gctcgcctgc gtggtggcgg tgctggtggc ctcgccgctg gaggtcagct cgtcacggcg
     6301 catcaaccag atggcgagca atggttacct gggcgtgctc cacgcagccg tgaccgacgt
     6361 ttctgcctgg cacggtcact actggaaccc ggcgcagccc gccccgacac gtggggaagc
     6421 acctggcgct ctgggggtgg tggagccggt ctggcagccg ctcccggtta agcatgtggt
     6481 gctgatcgtg gaggaatcct tctccggcga ggtttgggac gatttggcgc ttcgtgggac
     6541 ctacatgcca cgtttctctt cactcgccga ttccggcgtg cttttcgaga acgtctactc
     6601 gacgggaacc cgcacgaccc gggggttgga ggccctgctg aacggctttc cgcccctgcc
     6661 gggcatcgcc gtcaatcagc gcggcgggtt cgaacggctg ccctccttgg cgcgggcatt
     6721 gagccttgcc gggtttcaca acgtcttcgt ttacggcgga tggcccggat tctcgaactt
     6781 tttcccctac tggcgtgccc tgggatacga agagctgacc agccgcgacg acttcccgga
     6841 gccgcgtttc gaaaccagct ggggcgtggc cgacgagcat ctgttcgaag ccatccgtga
     6901 gcacatgagc cgtcttaccc agaaccacga gcgggtgttc ctgacgtcgc tgacggtcag
     6961 ccatcatcgc ccgttcgact ttccggcagg gcgggtcccg tttccggccg aggaacgccg
     7021 ctcggagtac gcgctggcct atgcggactg ggccctgggc cagttcattg aagatgcgag
     7081 cggggaaccc tggttcgacg acaccctgtt cgtcatcgct gccgaccacg gcccccgcat
     7141 cagcggcgac gcgcccattc cggtcgcggg ttttcgcgtt ccgctgctgc tgtacgcgcc
     7201 agctcacctg aacccgcgtc gggtcgacca tctggggtcg ctgatgagtt tcggcgtcac
     7261 gctgctcgag ctgctggggc ttccgaacga cgaaggcctc tacggagaca atcttctgct
     7321 caccgaacag ggcctcgtgc ccatggagca tgaatacc
//
LOCUS       DBNHOX010000612         9278 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1462967, whole genome shotgun sequence.
ACCESSION   DBNHOX010000612 DBNHOX010000000
VERSION     DBNHOX010000612.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9278)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9278
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1462967"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="AC16UZ_13405"
     CDS             complement(<1..518)
                     /locus_tag="AC16UZ_13405"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6247727.1"
                     /translation="MGLLFQAVVFGIGFFCFTFWVAPWRAEFGSSRGEVMAILVTVQI
                     VMGCAAPFAGRAMDHLSIRALVVAGAVCFAAALALAAHATALWQIGLIYSVLFVAGAL
                     LAGPLAAQTLAAKWFFARRGTAIGLSSVGTSLGGFVLPLLVTWLIAAYGWRQANLVLA
                     ALVVVVVVPVVWL"
     gene            complement(606..1361)
                     /locus_tag="AC16UZ_13410"
     CDS             complement(606..1361)
                     /locus_tag="AC16UZ_13410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6247728.1"
                     /translation="MRLGALLGPIVDASEPNALADQARRYAGEGFESLWSAQAIGRGA
                     MYTDPFVALSVAAAVTEKVELGTAVVQVPLYHPVDLAHRALSLRQVAGERVLLGVGAG
                     STQTDFDAFGRDYASRFRTFGESVEVLRQLFEEGRAGETNLSPWPAVLGGLPLLLGSW
                     GKGVERAAASFDGWIASAHYRTPDQVVEALDGYRAAGGRRAVVSTILLGAGTDLGETG
                     DLLGRFAEAGFDDAVVMLLPGGPSAADVRRLVA"
     gene            complement(1392..2612)
                     /locus_tag="AC16UZ_13415"
     CDS             complement(1392..2612)
                     /locus_tag="AC16UZ_13415"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6247729.1"
                     /translation="MNEPSGPLADITIIDCTMALAGPFGAAILADLGANVIKVEPPGG
                     DVARSVPPLPEDYANANSDQSAGCDFGGYFASVNRNKRSVVIDLKTPAGRETLLALCD
                     KADAIIENMRTGVMDRLGLGYEVLRARNPALVYGCIRGFGDPRTGRSPYAEWPAYDIV
                     AQSMGGIAHITGPESSHGFPAGASIGDLYPGTLLALGVVAAVHHARRTGEGQFMDVGM
                     YDAVMFLSETVIANFGYAGTELGPRGAHHPNLCPFGIFPTSDGAVAIAAPGPGHWQAL
                     CQAIGRPDLVDDEKTRNTFVRRKHQHFVEGVVSDWTATRTRAEVVAALGGRVPCGPVN
                     TAADIFADPHVAARGMVSQVELPGENGEVSIVGTPIKFTGTPTGLYRRPPMLGEHTEE
                     VLREMGLKGAANDE"
     gene            2750..3625
                     /locus_tag="AC16UZ_13420"
     CDS             2750..3625
                     /locus_tag="AC16UZ_13420"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6247730.1"
                     /translation="MGMLDGKVALVTGAGGGLGRAHALLLAQHGAAVVVNDLGGARDG
                     SGEGHAMADQVVAEIEAAGGRAAADYGSVSDPAAAKQMVQIAVDRFGKIDICINNAGI
                     LRDKSFKNMTDDMWDVVVDVHLRGTYLVAKAAWDQMIEQGTGGRIINTSSTSGLIGNF
                     GQANYGAAKAGIAGLTRVLALEGMKYGITVNTLAPAAWSRLTEDIMPEGMEERLAPEK
                     VSPAVVWLCTDDAANVTGRQFCAGQNRVSLLSWQVTEIADKNAMDAPWTVEEIGERMQ
                     ATMDAWPKLLKPMEV"
     gene            complement(3633..4427)
                     /locus_tag="AC16UZ_13425"
     CDS             complement(3633..4427)
                     /locus_tag="AC16UZ_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HIR6247731.1"
                     /translation="MIVEQIWTGNSYRNFNYLIVCPESGEALAIDPLDHKKCLARARD
                     KGWQITQVLNTHEHPDHTGGNGAVIEATGAKLLAHANAQRSIKGMDVALNAGDVVKVG
                     RTVELLALDTPGHTMAHVCLLSQTETPALFCGDTLFNAGAGNCHGGGHPDELYQTFAS
                     QLDQLPETTLVYPGHDYITNNLAFTLDREPDNEAAAALLKELEANHDPDAALVTSMAQ
                     EKKINTFFRLTSRTVIDRLREAFPDLPDEPTPHEVFVKLRELRNSW"
     gene            complement(4482..5276)
                     /locus_tag="AC16UZ_13430"
     CDS             complement(4482..5276)
                     /locus_tag="AC16UZ_13430"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6247732.1"
                     /translation="MKTQRLFLLIMVCALSACATSPMGRSQLILFPDSEMDQMGVAAY
                     REMQQQTPREQTAGVNDYVQCVVEHVVLAMPDGRMADWEVTVFADDQANAFALPGGKI
                     GVYTGLLDVAENQDQLATVIAHEVAHVLARHSNERVSTTYATQTGLQVVQIAGGTSSP
                     ARNQLYGLLGLGAQVGVLLPFSRAQESEADLMGLDLMAEAGFDPRESVRLWENMAESG
                     GARPPEFLSTHPSGETRMRDLNERMRRAMDLSLQARSAGRNPDCRR"
     gene            5447..5662
                     /locus_tag="AC16UZ_13435"
     CDS             5447..5662
                     /locus_tag="AC16UZ_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247733.1"
                     /translation="MTDIPDVGAQDPELKNSPIALESDEETVLLREEIPDEPFCYFNS
                     EAFPNGAVVKSGPMKLRCDLGIWVPIG"
     gene            complement(5708..6541)
                     /locus_tag="AC16UZ_13440"
     CDS             complement(5708..6541)
                     /locus_tag="AC16UZ_13440"
                     /inference="COORDINATES: protein motif:HMM:NF026066.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR6247734.1"
                     /translation="METASREDVNLWVAGSLKEMASLLEDQAANPFRINAYRRAARTL
                     EGLDEPVDALYRRSGREGLMALPSIGEGIATAVENLLRTGRLPQLERLRGSVEPETVF
                     DSLPGVGPRLAAAIHDTLHVDTLEELEVAAHDGRLQTVPGIGPSRAASIRALLAERLK
                     RPPRRALARAPSVAAILDADGQYRREAAAGRLRTIAPRRFNPSHKAWLPVLHTDRNDW
                     HMTLLFSNTARAHELGKTDDWVVVYYYDGDHNEGQCTVVTETAGPRRGARVVRGRENE
                     A"
     gene            6756..7280
                     /locus_tag="AC16UZ_13445"
     CDS             6756..7280
                     /locus_tag="AC16UZ_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6247735.1"
                     /translation="MSESQISVESAKPAIRAEQAEPAEESKGLATAPRERDMERLFED
                     FFDKRWLRPWSREWPGFESILEKKGPKVDVVDREHEIFVRAELPGMSKDDIEVSVSDD
                     SLSIKGSSHKEEETEEGDYHRREISTSYVARTVSLPARVRGDDAKATLRDGMLEVTIP
                     KAEDSKRRRVEVES"
     gene            7295..9049
                     /locus_tag="AC16UZ_13450"
     CDS             7295..9049
                     /locus_tag="AC16UZ_13450"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HIR6247736.1"
                     /translation="MRTDIAREVWEARYRFYRSGEPVDQTIEDTWQRVADTIASRERE
                     PGRWASAYRDLLGDFCFLPGGRILAGAGTGRRVTLCNCFVMGTIEDAMEPIFTALKEG
                     ALTMQAGGGVGYDFSTLRPVGTPARETGNTASGPVSFMHIWDTMCATLCSTGARRGAM
                     RATLRCDHPDIHAFVDAKRDPDALTHFNCSVLVTDAFMNALRDDAEWPLVFPAHHLPT
                     DQGTDIIRREWPGEDGAVPCAVVRRVRARELWEQIMRATYDCSEPGVLFIDRINHENN
                     LSYCERLSATNPCGEVPLPAYGACDLGSFNLTSFVRDPFAAGAAFDFDALRRRVPEAV
                     RFLDSVIDVSGYPLPQQAEQARASRRIGLGFTGLGDALIMLGLHYGSDAARELAAKVM
                     TCIRHEAYAASVALAREKGPFPLFQADAYLQSPGIQRLPESLRRDIARDGIRNSHLTA
                     VAPTGTISLLANNVSSGIEPVFAAEYRREVRGADGTSAVRTVRDFACQLWRDTGRSEA
                     RPPAFVSAEALKPADHLAMQAAVQPHVDHAISKTVNVPADSSFEEFEEIYAEAHRLGL
                     KGCTTYRPGGSRAAVLKH"
BASE COUNT         1633 a         3135 c         3047 g         1463 t
ORIGIN      
        1 agccagacca cgggcaccac cacgaccacc accagggcag ccagcaccag gttggcctgg
       61 cgccacccgt aggcggctat gagccaggtc accagcagcg ggagcacgaa gccgcccagg
      121 gaggtcccca ccgacgacag gccgatggcc gtgccccgac gggcgaagaa ccacttggcc
      181 gccagggtct gagccgccag cggtcccgcc agcagcgcgc cggcaacgaa cagaaccgaa
      241 tagatgaggc cgatctgcca cagcgcggtg gcgtgagcgg caagcgccag ggccgcagcg
      301 aaacagaccg cgcccgcaac caccagagcc cggatcgaca ggtggtccat ggcgcggccc
      361 gcgaagggtg ccgcgcagcc catcacgatc tgaacggtga ccaggatggc catgacctcg
      421 ccccggctgc tgccgaactc cgcgcgccac ggcgccaccc agaaggtgaa gcagaaaaag
      481 ccgatgccga agaccacggc ctggaacaaa agccccacgc ccagcacgtt ccagccgtag
      541 taccagcggg gttcggttcg tgctcgcggc gtgccgtcga tgttgcgggc tccccggggc
      601 ctgggtcagg cgaccaggcg ccggacgtcc gcggcggatg gcccgccggg caggagcatg
      661 accacggcgt catcgaatcc agcctccgca aagcggccga gcaggtcgcc ggtttcgccc
      721 aggtcggtgc cggctccgag caggatggtg gacaccacgg cgcgccttcc accggcggcg
      781 cggtagccat ccaacgcctc caccacctgg tccggggtgc ggtagtgggc agaagcgatc
      841 cagccgtcaa atgacgcggc cgctcgctcg acgcccttgc cccacgagcc cagcagcagc
      901 ggcagcccgc cgagcacggc cggccaggga gagagattgg tctcccccgc acgcccttcc
      961 tcgaagagct gcctgagcac ctcgaccgac tcgccaaacg tccggaagcg ggaagcgtag
     1021 tcccggccga aggcgtcgaa gtccgtctgg gtcgaccccg cccccacccc gagcagcaca
     1081 cgctcgccgg ctacctgccg cagcgaaagc gcgcggtgag ccagatcgac cgggtggtaa
     1141 aggggcacct gaaccaccgc agtgccgagc tccacctttt cggtcaccgc cgccgccacg
     1201 gaaagcgcga cgaaggggtc cgtgtacatg gcgccacgcc cgatggcctg ggccgaccag
     1261 agactttcga aaccctcgcc agcgtatcgg cgcgcctgat ccgccagcgc attgggctcg
     1321 gacgcgtcga cgattggtcc cagcaatgcc ccaagcctca tgacaactcc tccttgttgc
     1381 gaattgatcg ctcactcatc gttggcggca ccttttaagc ccatttctcg aagcacctct
     1441 tccgtatgct cgcccagcat gggcggtcgg cggtagaggc cggtgggggt acccgtgaac
     1501 ttgatggggg tgcccacgat ggaaacttcc ccgttttctc ccggcagttc cacctggctg
     1561 accatgccgc gcgccgccac gtgggggtcg gcgaagatgt ccgcagcggt gttgaccggc
     1621 ccgcacggca ccctgccccc gagcgcggcc accacctcgg ccctggtgcg cgtcgccgtc
     1681 cagtctgaca caacgccctc cacgaaatgc tggtgtttgc gccgcacgaa cgtgttgcgc
     1741 gtcttctcgt cgtccacgag gtcgggccgg ccgatggcct gacagagcgc ctgccagtgt
     1801 cccggaccag gagcggcgat ggccaccgcg ccgtccgagg tcgggaaaat gccgaacggg
     1861 cagaggttgg gatgatgcgc gccccggggg ccaagctccg tgccggcgta gccgaagttg
     1921 gctatcaccg tctcggagag aaacatcacg gcatcgtaca tccccacgtc catgaactgc
     1981 ccttcgcccg tccgtctggc atggtggacg gccgcgacca ctcccagggc gagcagagtc
     2041 cccggataga gatcgcctat gctggcgcca gccggaaacc cgtgagagct ctcaggcccc
     2101 gtgatgtgcg cgatgccgcc catgctctgc gccacgatgt cgtaggcggg ccactcggcg
     2161 tatggactgc ggcccgtgcg cggatccccg aaaccccgga tgcagccata gaccagggcc
     2221 gggttgcgcg cccgcagcac ctcgtagccg agcccgaggc gatccatcac cccggttcgc
     2281 atgttctcga tgatggcatc cgccttgtcg cacaacgcca ggagggtctc gcgccccgcc
     2341 ggggtcttca gatcaatgac cacactgcgc ttgttgcggt tgacgctggc gaagtagccg
     2401 ccgaaatcgc agccggccga ctggtccgag tttgcgttgg cgtaatcctc gggcagcggc
     2461 ggcacgcttc gcgccacatc cccgcccggc ggctccacct tgatgacgtt ggcgccgagg
     2521 tcggcaagaa tcgccgcacc aaacggcccc gccagcgcca tggtgcaatc gatgatggtg
     2581 atatcggcca gcggcccaga gggctcgttc atgcgccatc tctcctcccg gagcaactat
     2641 acccgcaccg atcgggccgg cacacctcga agcgcccgtg gccgggcgac cccgcttttc
     2701 gctagaattt tcgatctcga caaacaggcg aaaccgccgg gggaagaaca tgggcatgtt
     2761 ggatggcaag gtagcgctgg tcaccggagc cggtggaggg cttggccgcg cccacgccct
     2821 gctgctggcg cagcacggcg ctgcggtggt ggtcaacgat ctgggcggcg ccagggacgg
     2881 ctccggagag gggcacgcca tggccgacca ggtggtggcc gagattgagg cggcgggcgg
     2941 cagggccgca gccgactacg ggtcggtgag cgatccggcc gcagcgaagc agatggttca
     3001 gattgccgtg gaccgattcg gcaagatcga catctgcatc aacaacgccg gcattttgag
     3061 agacaaatcg ttcaagaaca tgaccgatga catgtgggac gtcgtggtgg acgttcacct
     3121 gcgcgggacc tacctggtcg ccaaggcggc ctgggaccag atgatcgagc agggaaccgg
     3181 cggccggatc atcaacacca gctccacctc cgggctcatc ggcaacttcg gtcaggccaa
     3241 ctacggcgcc gccaaggcgg gcatcgccgg gctcacccgg gtgctggcgc tggaggggat
     3301 gaaatacggc ataaccgtca acaccctggc cccggccgcc tggtcacgcc tgaccgagga
     3361 catcatgccc gaaggcatgg aggaacggct ggccccggag aaagtctctc cggccgtggt
     3421 ctggctgtgc accgacgacg cagccaacgt taccggccgt cagttctgcg ccgggcagaa
     3481 ccgggtctcg ctgctctcct ggcaggtgac cgagatcgcc gacaagaacg ccatggacgc
     3541 gccgtggacg gttgaggaga tcggcgagcg catgcaggcc accatggacg cctggcccaa
     3601 gctgctcaag cccatggagg tctaggccgc ggctaccagg agttcctgag ctcgcggagc
     3661 ttcacgaaca cctcatgagg ggtgggctcg tccggcaggt ccggaaacgc ctcccgcagg
     3721 cggtcgatga ccgtgcggct cgtcagccgg aaaaaggtgt tgatcttctt ttcctgggcc
     3781 atggacgtca cgagagcggc gtccgggtcg tgattcgcct ccagctcctt cagaagcgct
     3841 gccgcggcct cgttgtcggg ctcccggtcc agggtgaagg ccaggttgtt ggtgatgtag
     3901 tcatgtcccg gatacacgag ggtggtttcc ggcagctgat cgagctgtga ggcgaacgtc
     3961 tggtagagct cgtcgggatg ccccccgcca tgacagttgc cggccccggc gttgaacagg
     4021 gtgtcgccac agaacagggc cggcgtctcc gtctgggaca gcaggcagac gtgggccatt
     4081 gtgtggcccg gcgtatccag cgcaagcagc tccacggttc tacccacctt gaccacgtcg
     4141 cccgcattga gcgcgacgtc catgcccttg atgctgcgct gggcgttggc gtgggccagc
     4201 agctttgccc cggtggcctc gatcaccgca ccgttgccgc cggtgtgatc cgggtgctcg
     4261 tgggtattga gaacctgggt gatctgccac cccttgtccc gcgcgcgggc caggcatttc
     4321 ttgtggtcca gagggtcgat ggccagcgcc tcgcccgact ccggacagac gatgaggtag
     4381 ttgaagtttc ggtaggagtt accggtccag atctgctcga cgatcatgcg cgtgcccgtt
     4441 tagtcgaaag cgtcagtgta acccacgcac gcgcccggga cttaccgacg acagtcgggg
     4501 ttgcgccccg ccgagcgtgc ctgcagggag aggtccatgg cgcggcgcat acgctcgttg
     4561 aggtcacgca tgcgcgtctc cccggagggg tgcgtcgaga gaaactccgg cggccgggcg
     4621 cccccggatt ccgccatgtt ctcccagagc ctcacgctct cgcggggatc gaagcctgcc
     4681 tcggccatga ggtcgagacc catcaggtcc gcctcgctct cctgggcccg gctgaacggc
     4741 aggagcacgc cgacctgagc gccgagccct agaagcccgt acagctgatt gcgcgcggga
     4801 gagctcgtcc cgccggcgat ctgcacgacc tgcaggcccg tctgggtggc gtaggtggtc
     4861 gatacccgct cgttcgagtg gcgcgccagc acgtgagcca cttcgtgcgc gatcacggtg
     4921 gcgagctggt cctggttttc ggccacatcg agcaagccgg tgtagacgcc gatcttgccc
     4981 cccggaaggg cgaaggcgtt ggcctgatca tcggcaaaga cggtgacctc ccagtcggcc
     5041 atcctgccgt cgggcatggc cagcaccacg tgctcgacca cgcactgaac gtagtcgttg
     5101 acgccggccg tctgctcgcg gggcgtctgc tgctgcatct cccggtaggc ggctaccccc
     5161 atctggtcca tttccgaatc cggaaacagg atgagctgac tgcgccccat gggcgaggtc
     5221 gcgcaggcgg acagcgcgca aaccatgatc aggagaaaga gtcgttgtgt cttcacggga
     5281 tgatccccat gtggcccgcg gaaaggatgc gcgaactgcc cgagagagac aacacgcgcg
     5341 ccaccctctc cgggcattgt agaatgtcca tctacggggt tgaccggatg ccattgcatt
     5401 cgttccgtcc gctagcatga ctcgaaagcc aacaggacac attccaatga ccgacatacc
     5461 ggacgtgggc gctcaggacc ccgagctcaa gaactcgcca attgcgctgg agagcgacga
     5521 ggaaaccgtg ctgctcagag aagagatccc ggacgagccc ttctgctact tcaattccga
     5581 agcatttcca aacggcgccg tggtaaagag cggcccgatg aagctgcgct gcgatctcgg
     5641 gatctgggtg ccgatcggct agcgctcaat cgggagcccg aaagggcgtc gggttcgacg
     5701 ccatgggcta ggcttcgttc tcacgcccgc gcacgacgcg cgcgcccctg cgcggccccg
     5761 ccgtctcggt gaccaccgtg cactggcctt cgttgtgatc gccgtcgtag tagtagacga
     5821 ccacccagtc atccgtcttg ccgagctcgt gggccctggc ggtgttggaa aagagcagcg
     5881 tcatgtgcca gtcgttgcgg tcggtgtgca acacggggag ccaggccttg tgggacggat
     5941 tgaaacggcg cggggcgatg gtgcgcagcc ggccggctgc ggcttcgcgt cgatattgcc
     6001 cgtcggcatc cagtatggcg gcgacactcg gcgcccttgc cagggcgcga cgcggcggcc
     6061 gcttcagccg ctctgcaagc agcgctctga tactggccgc ccggctgggg ccgatgccgg
     6121 gaaccgtctg cagacgaccg tcgtgggcgg ccacttccag ctcctccagg gtgtccacgt
     6181 gcagggtgtc gtgaatggct gccgccaggc gcgggccgac gccgggcagg gagtcgaaca
     6241 ccgtctccgg ctccacgctg ccgcgcagcc gctcgagctg gggcagcctg ccggtgcgca
     6301 ggaggttttc gaccgccgtg gcgatgcctt cgccgatgga aggcagcgcc atcaacccct
     6361 cgcgccccga gcgccggtag agcgcatcca ccggctcgtc caggccctcc aaggtccgcg
     6421 ccgcccgccg gtaggcattg atacggaacg ggttggcggc ctgatcctcc agtaggctgg
     6481 ccatctcctt caaagagccg gccacccaga ggttcacgtc ttcacggctt gccgtttcca
     6541 cggtaaggct tcacccgttg caactccggg caatgtgaca tgcctgccgg gcccgcgtca
     6601 tcaggtattt cacctagacg gcgcggtcgg ccggccgcgc cgaatcgccg cccggttccc
     6661 tcgagaaaac cggatccggg taagtatttg ttcggataca cgtgccttcc acagcgggca
     6721 gataatcccc cgtggactga ttgaggagat gtgaaatgag cgagtctcag atttccgtgg
     6781 aaagcgccaa gccggcaata agggccgagc aggcggaacc tgccgaggaa agtaaagggt
     6841 tggcaacggc accgcgcgag cgcgacatgg aacggctctt cgaggatttc ttcgacaagc
     6901 gctggctgcg gccgtggagc cgcgagtggc ctggattcga atcgattctg gaaaagaagg
     6961 gcccgaaggt ggacgtggtg gaccgcgagc acgagatctt cgtccgtgcc gagctgccgg
     7021 gaatgagcaa agacgacatc gaggtgtccg tcagcgatga cagcctcagc atcaagggct
     7081 cctcacacaa ggaagaggag accgaggagg gtgactatca tcgccgcgag atctccacga
     7141 gctacgtcgc ccgcaccgtt tcgctgccgg cgcgggttcg gggcgacgat gccaaggcga
     7201 cgctgcgcga cggcatgctg gaggtcacga ttccaaaggc ggaggactcc aagcgccgtc
     7261 gggtcgaagt cgagtcgtag ccatctcgga ccctttgcgg accgacatcg cgcgggaggt
     7321 ctgggaggcc cgctaccgct tctatcgaag cggcgagccg gtcgatcaaa ccatcgagga
     7381 tacgtggcag cgtgtcgcgg acacgatcgc ttcccgggag cgggagccgg gccgttgggc
     7441 ttccgcttac cgggatctgc tgggtgattt ctgcttcctt cccgggggcc ggatcctggc
     7501 tggcgctgga accgggcgcc gggtcaccct ttgcaactgc ttcgtcatgg gcaccatcga
     7561 agacgcgatg gagcccatct tcaccgctct gaaggaaggc gcgctgacca tgcaggcggg
     7621 cggcggggtc ggctacgact tctccaccct gcgcccggtg ggaacgcccg cccgggaaac
     7681 cggcaacacg gcctccgggc cggtctcgtt catgcacatc tgggacacca tgtgtgccac
     7741 cctgtgctct accggagccc gccgcggcgc catgagggcg acccttcgct gcgatcatcc
     7801 cgacatccat gccttcgtcg atgccaaacg ggaccccgac gccctgaccc atttcaactg
     7861 ctcggtcctc gttaccgatg cgttcatgaa cgcgttgcgc gacgacgccg aatggcccct
     7921 ggtctttccc gcccaccacc tgccgacgga ccagggcacc gacatcatcc ggcgcgaatg
     7981 gcccggggaa gatggagccg tcccgtgcgc cgtggtgcgc agggtccggg cgcgcgagct
     8041 gtgggaacag atcatgcggg cgacgtacga ctgctccgag ccgggggtgc tgttcatcga
     8101 ccgcatcaac cacgagaaca atctcagcta ctgcgagcgc ctctcggcca ccaatccctg
     8161 cggcgaggtc cctctgcccg cctacggcgc ctgcgatctc ggcagcttca acctcacgtc
     8221 gttcgtgcga gacccgttcg ccgcgggcgc cgccttcgac ttcgacgccc tgcgccgccg
     8281 agtgcccgag gccgttcgct ttctcgattc ggtcatagac gtttccggct atccgctgcc
     8341 gcagcaggcg gagcaggcgc gcgccagccg ccgcatcggc ctcggcttca cgggtctcgg
     8401 cgacgccctg atcatgctgg gccttcacta cgggagcgac gccgcgcgcg agctggcagc
     8461 gaaagtcatg acgtgcatcc gacacgaagc ctacgcggcc agcgtggcgc tggcccggga
     8521 aaaggggccc tttcctttgt ttcaggctga cgcgtacctg caatcgccgg gcatccaacg
     8581 actgcccgaa agtctccgcc gcgacatcgc ccgggacggg atccgcaaca gccacctgac
     8641 cgccgtcgcg cccaccggaa ccatcagcct gctggcgaac aacgtctcca gcggcatcga
     8701 gcccgtcttc gccgccgagt accggcgcga ggtgcgcggc gccgatggca cctccgccgt
     8761 tcgaaccgtc cgtgacttcg cctgccagct gtggcgcgac accggcagga gcgaggcgcg
     8821 gccgccggcg ttcgtgagcg ccgaagcgct caaacccgcc gatcatctgg ccatgcaggc
     8881 ggcggtccaa ccccacgtgg atcacgccat ctcaaagacc gtcaacgtgc ccgcagactc
     8941 gagcttcgag gagttcgagg agatctacgc cgaggcgcac cgcctgggcc tgaaggggtg
     9001 caccacctat cggcccggcg gctcccgggc ggcggtgctc aagcattgaa ccgaggagcg
     9061 cggactgctt aaaaaaaatc gagcgcgctt gccgtcgatc ctgccgttac aatgacggtt
     9121 tgtgatcagg cagcgtggac gatcatggcc gaagaaacca gcaagcaccc caccccgccg
     9181 aaaacccagc gggagttcat gcagaagacc aatcgggaga tggcggccga gtgggccatc
     9241 gacgtcgctc aggaccccta ctccctgcca atcgaatc
//
LOCUS       DBNHOX010000613         2869 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_179391, whole genome shotgun sequence.
ACCESSION   DBNHOX010000613 DBNHOX010000000
VERSION     DBNHOX010000613.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_179391"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="AC16UZ_13455"
     CDS             <1..903
                     /locus_tag="AC16UZ_13455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6247737.1"
                     /translation="GRLKEDELVADIIPEFAANHKHDITVGQLFVHTAGFPSAPFKPL
                     DWNDRARRLERFAQWQLNWPPGSRYEYHPTSSMWVIAEIIERRANTTYQEFVRERIAL
                     PLGLPELYVGLPATENERVVPNVHVGDALTRADYELLGLPEPPVTEVTEEAILKFNRP
                     EVRAVGVPGGGGIMSAADLALFYQGLLHGGLGEEVWSAATLADARRVRSGELRDPVMG
                     HRANRALGLVIAGDPQRNLRGFGKTNSPETFGHNGAGGQLAWADPATGLSLAYCTAGH
                     DRNRIRQGRRGVAISSLAAVCCED"
     gene            complement(1426..1821)
                     /locus_tag="AC16UZ_13460"
     CDS             complement(1426..1821)
                     /locus_tag="AC16UZ_13460"
                     /inference="COORDINATES: protein motif:HMM:NF039797.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HIR6247738.1"
                     /translation="MPSTSKFAKLACSIAGTVLLYASSGLALPPDSPIGPRAESIVEG
                     TVQAPVSSIPAANRIEGWARVDDKRVVLEVSPQTRYLLTLRQGCIGLGFARNVSVTMS
                     NNTIYAGFDAITADGEHCEIRTINPLVTP"
     gene            2109..>2869
                     /locus_tag="AC16UZ_13465"
     CDS             2109..>2869
                     /locus_tag="AC16UZ_13465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6247739.1"
                     /translation="MAASAESVLERVDAEAAAAVSMAERLWALAELGYQETRSSELLG
                     AYLAESGFSLESGVAGIPTAFVATFGSGRPVIGVLAEFDALPGMSQAAEPSRRPLEPG
                     GAGHACGHHLFGAGAAAAAVSVARWMEANGVPGSIRVYGTPAEEGGSGKVYLTRAGLF
                     DDVDVVLHWHPGSRNDASPATTTANKSGRFTFRGVAAHAAAAPERGRSALDGVESMNY
                     MVNLMREHVPSDSRIHYVITKGGDAPNIVPEIAQV"
BASE COUNT          495 a          908 c          983 g          483 t
ORIGIN      
        1 gggcgcctga aggaggatga gctcgtagcc gacatcatcc ccgagttcgc cgcgaaccat
       61 aagcacgata tcaccgtggg gcagctgttc gtgcacaccg ccggctttcc gtccgcgccc
      121 ttcaagcccc tggactggaa cgaccgcgcg cgccgtttgg agcgttttgc ccaatggcag
      181 ctgaactggc cgccgggcag ccgctacgag taccacccga cctcctcgat gtgggtgatc
      241 gcagagatca tcgagcggcg cgcgaatacc acctatcagg agttcgtccg cgaacgcatc
      301 gccctacccc tggggctgcc cgagctctac gtggggctcc cggcgaccga gaacgagcgg
      361 gtggtgccga acgtgcatgt gggcgatgcc ctgacgcgag cggactacga gcttctcggt
      421 ctgccggagc cgcccgtgac tgaggtgacc gaggaagcga tcctgaagtt caaccggccg
      481 gaagtccgcg cggtcggggt cccgggcggg ggcgggatca tgagcgcagc ggatctggcg
      541 ctgttctacc agggattgct gcatggtggc ctgggcgagg aagtctggag cgccgccacc
      601 cttgccgacg cccgtcgcgt gcgcagcggc gaactccgcg acccggtgat gggccaccgc
      661 gccaatcgag ctctgggcct cgtcatcgcg ggagatccgc aacggaatct ccgcggtttc
      721 ggcaagacca actccccgga gaccttcggc cacaacggag ccggcgggca gctcgcctgg
      781 gccgatccgg cgaccgggct gtccctggcc tactgcacgg ccggtcacga tcgcaaccgg
      841 atacgccagg ggaggcgggg cgtagcgatc tcgagcctgg ctgccgtgtg ctgcgaagat
      901 tgaccaggac gcaacaccgc gcgcaggaac cgcttgattt gcttctcaat ctgctccctg
      961 gcgtcgggga gcgcggaacg aactgtgaca gacgcctctg tccccgcagg gcggctgaac
     1021 taatctgccg actacgctga ttgccaccgg cacagcgaga cgcgagagga gtcaccggcc
     1081 cgtgcggcgg tgaacttaac ggttacaccg ggattcgctg tcgattcttc ggggcttccc
     1141 cgacgggtcg agcagtcggc gagttccggt tttacatccg gcgtcgaccc gatgtccggg
     1201 tcaaggatct cccggcatca gcccgaccgc caggacggcg gaggcgaaca gagcaaggac
     1261 tgcggaaacg gtggttgccg gtagttcgaa ggatttagcc gaaaggattt cggctccgaa
     1321 gggacttcgg agtggtgcaa atggatctca ggagcatgga gcacccaatc tttcgacggc
     1381 acactgttgt ctcccccgcg tctgaaagcg gcgcatcccc gcgcctcatg gggttaccaa
     1441 cgggttgatg gtgcggatct cgcaatgttc gccatcggcg gtgatggcgt cgaacccggc
     1501 gtagatggtg ttgtttgaca tggtcacgga cacgtttcgc gcgaatccca gaccgatgca
     1561 tccctggcgc agggtcagca ggtatcgcgt ctgcggactg acctccagca cgacccgctt
     1621 gtcgtccact cttgcccagc cttcgatccg gttggctgcc gggatgctcg agacgggggc
     1681 ctgcacagtg ccctcgacaa tggactccgc gcgggggcca atgggagagt ccggcggcag
     1741 ggcgagtccg gacgacgcgt agagcagtac tgtcccggcg attgagcaag caagttttgc
     1801 aaatttcgag gtgctgggca tgacgttttc ctctggcaag ggcgacgttc aggctatgag
     1861 cgcgacgctg aatgtcggct gaacatgagg caacggtacg ggagcccgtg tgaaaaggtc
     1921 gtgagaccgc ttgagggggc tgatgcggtt cgagggttgg caacgccggg gatattccgc
     1981 aaagtgtcca cgaggcgaac cagcactggg ggtacggttt gatcgatcgg ggagcacatc
     2041 ggcgccgcgc cgcaatcctg aatgaggccg cccggcggat cgttctgctc aacctggcga
     2101 tattcagcat ggccgcgtcg gccgagtccg tcctcgagcg ggtcgatgcg gaagccgccg
     2161 ccgccgtatc gatggccgag cgcctctggg cgctggctga gctcggctat caggaaaccc
     2221 gcagctccga gctgctcggg gcctatcttg cagagagcgg attctcgctc gaaagcggcg
     2281 tggcggggat acccacggcc tttgtcgcga cattcggttc cggccggccc gtcatcggcg
     2341 tcctcgcgga gttcgacgcg ctgccgggca tgtcacaggc cgcggaaccc tccagacgac
     2401 ccctggagcc cggcggggcc ggccacgcct gcgggcatca cctgttcggc gcaggggcgg
     2461 cagcggcagc ggtttccgtg gcgcgctgga tggaggcaaa cggggtgcct ggctcgattc
     2521 gcgtgtacgg cacgcccgcc gaggaaggcg gttccgggaa ggtctacctc accagggcag
     2581 gccttttcga cgacgtggac gtcgtgctcc actggcaccc cggcagccgc aacgacgcct
     2641 cgccggcaac caccacggcg aacaagtcgg gccgcttcac tttccgggga gtggccgcgc
     2701 atgccgccgc tgccccggag cgcgggcgat ccgctctgga cggcgtggag tccatgaact
     2761 acatggtgaa tctcatgcgc gagcacgtgc cgtctgacag ccgcatccac tacgtgatca
     2821 ccaagggtgg cgatgcgccg aacatcgtcc cggaaatcgc ccaggtcta
//
LOCUS       DBNHOX010000614         4318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1143523, whole genome shotgun sequence.
ACCESSION   DBNHOX010000614 DBNHOX010000000
VERSION     DBNHOX010000614.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1143523"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="AC16UZ_13470"
     CDS             <1..435
                     /locus_tag="AC16UZ_13470"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6247740.1"
                     /translation="GHMAFPGGHRDPEDESLRAAAERETLEEIGLDLDSARYLGPLNQ
                     QRAVARGRPLNMLIAPHVFEIAGDPSFTINYEVAQVVWTPLAPLVSGALHDTETRPLA
                     GTPTVFNGYRLPQGHFVWGLTYRMVKSFFAAIDPGWEPPPEV"
     gene            complement(452..1840)
                     /locus_tag="AC16UZ_13475"
     CDS             complement(452..1840)
                     /locus_tag="AC16UZ_13475"
                     /inference="COORDINATES: protein motif:HMM:NF012242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR6247741.1"
                     /translation="MRVGVGIDFGTTNSAAATYDGERLQLVALEGADPIMPSATYIDR
                     ELQTLTGQAAINQYIADNTGRTVELVPEVIAETSAFVEHGDSENPAPVETVTQKIYGQ
                     PLIDSGLHGRLFRGTKRLLGDPRVRRLMVFDHPFRLVALITPILLRMRKALSRTVSGA
                     DYAHLGHPVNFEGRDEFRNQTALARLGEAYKYAGITEQRFYPEPIAAAVSYLFAHPEA
                     AGEHLLTLDFGGGTLDFCILRRAAGDFEVVTTHGIALGGDHIDQRLFRELLFPLLGKG
                     ETWRRRGMDREIETVFPFEDYQELLVNWAVTYTLNQNRYTTPVLECIGQGGPGARKFR
                     RLRELIQQNLSYVVFQTIKDFKADLSAREEAVLDISEIDVEVRLTRAGFESLIGDLLE
                     QAEQALDETVALAGLVHGDVDIVLCTGGSSLIPAVRRILEDRFPGRVVEHDPFTSVAA
                     GLAIANFHGMDV"
     gene            complement(1910..2293)
                     /locus_tag="AC16UZ_13480"
     CDS             complement(1910..2293)
                     /locus_tag="AC16UZ_13480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HIR6247742.1"
                     /translation="MGTSLDDARYFNLATFRKNGSAVETPVWFAPSAGEAPGVYYVFS
                     AGDAGKVKRLRRDQTARVAACDVKGKLQGEWLQARAELIEGADAQAALVALRLRYGWQ
                     MRLADLLSRLTGRFRRRAYIRVTLA"
     gene            complement(2338..2991)
                     /gene="msrA"
                     /locus_tag="AC16UZ_13485"
     CDS             complement(2338..2991)
                     /gene="msrA"
                     /locus_tag="AC16UZ_13485"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR6247743.1"
                     /translation="MAWEQKLRIPSESEALPGRAEEMPVPAAHHVNGAPLKEPFPEGL
                     AQAVFALGCFWGAERCFWNTPGVYSTAVGYAGGSTPNPTYEEVCSGLTGHTEVVLVVY
                     DPQKVSYAELLKVFWESHDPTQGMRQGNDVGTQYRSAIYTFDDEQAAAARASADEYQK
                     ALSAAGFSEITTEIRSAPAFYYAEPYHQQYLAKVPNGYCGLGGTGVGCNVAPLVKAV"
     gene            3125..>4318
                     /locus_tag="AC16UZ_13490"
     CDS             3125..>4318
                     /locus_tag="AC16UZ_13490"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6247744.1"
                     /translation="MLAASMVGCQSMPLDYAGLNDKIANGDQVAVGELRRAFLDLEDL
                     PERMERLRDLELQAAQLFEDEPLKLGSIGTAILDTYYGSLTGHFALSRFYEFVGSEEA
                     AVPHRQWQARIREDMEASADGSRESPYPAVTDVEAQMYAVSLGLSPVGSIYQTGEAFP
                     YTLLIQARPADGPLRNLYFDLGGWYESMRRQFPDENEFSPFALIGFQARRGDTAAQVA
                     VGAFLATQGRGDEAVDWLRAASRTGNLLANSLLARIFWEEANKDGEDDARQAALDEVL
                     ENYLHAVALGSADAMYALGVLYMNEHYGAENRDSGVPLLRQAAELEHSDAAMLLAHMH
                     YTGEGVERDLDRATDYYVASAESGNDFARRSYARFLLAEAREDPRALQWLEELAEDDD
                     AEAMIL"
BASE COUNT          780 a         1441 c         1363 g          734 t
ORIGIN      
        1 gggcacatgg cttttccagg cgggcaccgg gacccggagg acgagagtct cagagcggcc
       61 gcagaacggg agactctgga ggagatcggg ctggacctcg attcggctcg ctacctgggt
      121 ccgctgaacc agcagcgcgc cgtggcccgc ggccgccccc tcaacatgct catcgcgccc
      181 cacgttttcg agatcgccgg ggacccttcg ttcaccatca actacgaggt ggcacaggtg
      241 gtctggaccc cactggcacc gctcgtatca ggggcgcttc acgataccga gacccgtccg
      301 ctggcaggca cgccgacggt gttcaacggc taccgcctgc cccagggaca ctttgtctgg
      361 ggtctcacct accggatggt gaagtcgttc ttcgccgcca tcgacccggg ctgggaacct
      421 ccgccggagg tctagcgagc gccgctgacg cctaaacgtc catgccgtgg aagttggcga
      481 tggccaggcc tgcggccacg ctggtgaacg gatcgtgctc cacgacccgc ccgggaaatc
      541 gatcctccag aatgcgccgc accgcgggaa tcaggctgga gccgccagtg cacagcacga
      601 tgtccacgtc cccatggacg agcccggcga gcgctaccgt ttcgtccagc gcttgctccg
      661 cctgttccag gaggtcgccg atcagcgatt cgaaacccgc acgggtcaaa cgcacctcca
      721 cgtcgatctc ggatatgtcc agcaccgctt cttcccgagc cgaaagatct gctttgaagt
      781 ccttgatggt ctgaaacacc acgtagctca agttctgctg gatcagctcc cgaagccggc
      841 ggaacttgcg cgcgcccgga ccgccctgcc ctatgcactc caagaccggc gtggtgtagc
      901 ggttctggtt gagcgtgtag gtcaccgccc agttgaccag gagctcctgg taatcctcga
      961 agggaaagac ggtttcgatc tcccggtcca taccccgccg gcgccacgtc tcgcctttgc
     1021 cgagcagcgg gaagagcagc tcgcgaaaca ggcgctgatc aatgtggtcg ccgcccagcg
     1081 cgataccatg ggtggtgacc acctcgaaat cgccggcagc ccgccgcagt atgcagaaat
     1141 ccagcgtgcc gccgccgaaa tccagggtga gcagatgctc accggcagcc tcgggatggg
     1201 caaagagata actcacggca gcggcgatgg gctccggata aaagcgctgc tccgtgatgc
     1261 ccgcatactt gtaagcctcc cccagtcgcg ccagagcagt ctggttgcgg aactcgtccc
     1321 ggccttcgaa gttcaccgga tggcccaggt gagcgtagtc ggccccgctc accgtccggg
     1381 agagagcctt acgcatgcga agcagtatcg gcgtgatgag ggcaaccagg cggaaaggat
     1441 gatcgaaaac catcaggcgc ctcacccggg gatcgcccag gagccgcttg gtcccccgga
     1501 acagccgccc gtgcaggccg ctgtcgatca gcggctgccc gtagattttc tgggtgacgg
     1561 tttccaccgg cgcggggttc tccgaatccc catgctccac gaaggcgctg gtctctgcga
     1621 tcacctctgg aaccagctcc acggttcggc cggtattgtc ggcgatgtac tgattgatgg
     1681 ccgcctgacc ggtaagcgtc tgcagctcac ggtcgatgta ggtggcggag ggcatgatcg
     1741 gatcggcgcc ctccagcgca acgagctgca gccgctcgcc gtcgtaggtg gccgccgccg
     1801 agttggtcgt gccgaaatca atgccgaccc ccacgcgcat cagttgccgc cctctcgttg
     1861 ccaattcatc tccaacgcct cgtcccggaa accttgccgg ccggcgcaat cacgcaagag
     1921 tcacccggat gtaagcacgc cggcggaacc gccccgtcaa tcgcgagagg agatcggcaa
     1981 gtcgcatctg ccacccgtac cggaggcgca gcgccacgag cgccgcctgg gcgtccgcgc
     2041 cctcgatcag ctccgcgcgt gcctgcagcc actccccctg cagcttgccc ttgacgtcac
     2101 aggcagcaac ccgcgcggtc tggtcacggc gcaaacgttt gacctttccg gcatcacccg
     2161 ccgagaagac gtagtaaacg cccggcgctt cgcccgccga gggagcaaac cacaccggcg
     2221 tctcgaccgc cgagccgttc ttcctgaagg tagccagatt gaagtagcgt gcgtcgtcga
     2281 gcgaggtgcc caccgacaat ctccgccgcg aagcaagcgg cggagtttat cgcgaattca
     2341 aaccgccttc acgaggggcg ccacgttaca gccgactccg gtccctccaa gaccgcaata
     2401 gccgttggga accttcgcaa ggtactgctg atggtacggc tcggcatagt agaacgccgg
     2461 ggcagacctt atctccgtcg tgatctcgga aaatccggca gcagaaagcg ccttctgata
     2521 ttcgtcggcg gacgcccgcg cggcggccgc ctgctcgtcg tcgaacgtgt agatcgccga
     2581 gcggtactgg gtgcccacgt cattgccctg gcgcatgccc tgggtggggt cgtgcgactc
     2641 ccagaagacc ttgagcagct cggcgtagga aaccttctga ggatcgtata cgacgagcac
     2701 gacttcggtg tgccccgtca gcccggagca gacttcctcg taggttgggt tcggcgtgct
     2761 gccgccggca tagccgacgg ccgtggagta caccccgggc gtgttccaga aacaccgctc
     2821 cgcgccccag aaacaaccaa gagcgaacac ggcctgagcg aggccctcgg gaaacggttc
     2881 cttgaggggg gcgccgttga cgtggtgtgc cgccggcacg ggcatctcct cggctcgtcc
     2941 cggcagagcc tcggattcac tggggatacg gagtttttgc tcccaagcca tgtccaattg
     3001 cctgtaacat tgaaggaaga tgaaaatcat acatttcgcc gccccctccg caactgccag
     3061 ttcgcccagc cgccgagcca ctcgcctctt ccccttcggg attcaggggg cggctctgat
     3121 cgtcgtgctg gcagcatcga tggtcggctg ccagagcatg cccctcgact acgccggcct
     3181 caatgacaag attgcaaacg gggatcaggt cgccgtgggc gagctgcgca gagcgtttct
     3241 cgatctggag gatctgccgg aacggatgga gcgtcttcgc gatctggagc tgcaggcggc
     3301 gcagctgttc gaggacgaac ctctgaagct gggatccatc ggcaccgcca ttctggatac
     3361 ctactacggc agcctgacgg gtcacttcgc gctttcgcgg ttctacgagt tcgtgggctc
     3421 cgaagaggcc gcggtacccc accggcagtg gcaggcgcgc attcgcgagg acatggaggc
     3481 gtccgcggat ggcagccggg aatcgcctta cccggcggtt accgacgtgg aagcccagat
     3541 gtatgccgtc agcctgggct tgagcccggt gggctccatc taccagacgg gcgaggcgtt
     3601 cccctacacg ctgctcatcc aggcgcggcc ggccgatggg cctctgcgca acctgtattt
     3661 cgatctgggc ggatggtacg agtccatgcg ccggcagttc cccgacgaaa acgaattttc
     3721 gccgttcgcg ctgatcggct ttcaggcccg gcgcggcgat accgcagccc aggtcgccgt
     3781 cggcgcgttt ctcgccaccc agggcagagg cgacgaagcc gtggactggc tgcgagcggc
     3841 ctcgcgtacc ggaaaccttc tcgccaactc ccttctcgcc cgaatcttct gggaggaggc
     3901 gaacaaggac ggcgaagacg atgcccggca ggccgcgctg gacgaggtgc tggaaaacta
     3961 cctgcacgcc gtcgcgctcg gctcggccga cgccatgtac gcgctgggcg tcctctacat
     4021 gaacgagcac tacggagccg agaaccgtga ctccggggtg ccgctgctgc gccaggcagc
     4081 ggagctggaa cacagcgacg ccgccatgct gctcgcgcac atgcactaca ccggcgaggg
     4141 cgtcgagcgc gatctggacc gcgcgacgga ctactacgtc gcttcagccg agagcggcaa
     4201 tgacttcgct cgtcgcagct acgcgcgttt cctgctggcc gaggcgcgcg aggatcccag
     4261 ggccctgcag tggctggagg aacttgctga ggatgacgat gcggaggcga tgatcctg
//
LOCUS       DBNHOX010000615         2339 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1206791, whole genome shotgun sequence.
ACCESSION   DBNHOX010000615 DBNHOX010000000
VERSION     DBNHOX010000615.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1206791"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="AC16UZ_13495"
     CDS             complement(<1..271)
                     /locus_tag="AC16UZ_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247745.1"
                     /translation="MCDEQTVRDNEAYLHTSAGMSRRQFAGAAAGAAIAMLLPRSGNA
                     LTVSGRDVRITTPDGEADAYFVHPAEGKHPGVIVWPDIRGLRPAFR"
     gene            complement(315..1349)
                     /locus_tag="AC16UZ_13500"
     CDS             complement(315..1349)
                     /locus_tag="AC16UZ_13500"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen
                     decarboxylase/cobalamine-independent methonine synthase
                     family protein"
                     /protein_id="HIR6247746.1"
                     /translation="MAALESGITGSLPKPKWLSDPSAQLRADWMVADDALGEAHDDAV
                     RLAIADQVSAGLDIVTDGEQRRRHYIWGFLEALEGVDAHKLGKKQTRGARYTTELQNV
                     ARVVGDVRRSAPIFVDALKFAKAHSDRPVKVTLPGPMTIVDSVVDEHYGDALPDLAAR
                     FADILNAEARALADAGADIVQFDEPAFNVDTDAVAEWGIATLERAARGVATQRAVHIC
                     YGYGVPQVIAWKNQNQDWGHYGHTLPLLNESSIDQVSVECAASGVDVSVIQHLSNKRV
                     MLGVISVSTDEVETPELVAERIRAGLEFCDAERLMPCTDCGLVPRPRAVAQAKMAALA
                     AGTALVRDAL"
     gene            complement(1377..1919)
                     /locus_tag="AC16UZ_13505"
     CDS             complement(1377..1919)
                     /locus_tag="AC16UZ_13505"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6247747.1"
                     /translation="MSNAPPVNSRLHHNAYVTENMEAVRRFYEEVIGFPLIATWCEQA
                     ELFGRERTYMHCFFDVGGGESLAFFQFADPADAAEFGPELPSSPFRHIAMKVDQETQD
                     AIRDRLAAAGYKEPDTYVLNHGYCYSLYVVDPSKLIIEFTVDHEDVDQINADQRAKAH
                     AELARWLGGDHSPNNEAYHR"
     gene            complement(1942..>2339)
                     /locus_tag="AC16UZ_13510"
     CDS             complement(1942..>2339)
                     /locus_tag="AC16UZ_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6247748.1"
                     /translation="YLGFGYVDSEIDEAANTDHEGNQAPLVSEYTLNVGLQYRQPLGA
                     MNGRLELVGRVDYQRIGDTYWEPANITKRSPVDLVDLRLGVEVPGDWALTAWAKNAFD
                     KKYNAEYSPGGFLFKAPPARWGVDFTKRF"
BASE COUNT          375 a          805 c          748 g          411 t
ORIGIN      
        1 gccggaacgc cggccgcagt ccccggatgt ccggccacac gattaccccc gggtgcttcc
       61 cttcggcagg atgcacgaag tacgcatcgg cctcgccgtc cggcgtggtg atccgaacgt
      121 cgcggcccga aaccgtcagg gcgttgccag agcggggcag cagcatcgca atcgccgcac
      181 ctgcagcggc ccccgcgaac tggcgccgtg acatgccggc agacgtatgc agatacgctt
      241 cgttgtcacg aacggtttgc tcgtcgcaca tggtcaagcc tccctggccg gccggcgacg
      301 gccgagccaa tcgatcagag ggcgtcccgc accagcgcgg tgcctgcagc cagagccgcc
      361 atcttcgcct gggccaccgc ccgggggcgc ggcaccagac cgcagtccgt gcaaggcatg
      421 aggcgctcgg cgtcgcagaa ctcgaggccg gcccggatgc gctcggccac cagctccggc
      481 gtttccacct cgtcggtgct cacgctgatc acgcccagca tcacgcgctt gttggacaga
      541 tgctggatca ccgagacgtc gaccccggac gccgcgcact ccacggacac ctgatcgatg
      601 ctggactcgt tcagcagcgg cagcgtgtgc ccgtagtgcc cccaatcctg attctgattt
      661 ttccaggcga tcacctgggg cacgccgtag ccgtaacaga tgtgcacggc gcgctgcgtc
      721 gccacgcccc gcgccgcgcg ctccagcgtg gcgatgcccc actcggccac cgcatcggtg
      781 tccacgttga aggcgggctc gtcgaactgg acgatgtctg ctccggcgtc ggcgagcgct
      841 ctggcctccg cgttgaggat gtcagcaaac cgcgcagcga gatcgggcag cgcatcgcca
      901 tagtgctcgt ccaccaccga atccacgatg gtcatgggtc cgggcagggt gacctttacc
      961 ggccggtcgg agtgggcctt ggcgaacttc agagcgtcca cgaaaatggg ggcggaccgc
     1021 cgcacgtccc caacgacgcg cgcgacgttc tgcagctcgg tggtgtaacg cgccccgcgc
     1081 gtctgcttct tgcccagctt atgcgcgtcc acgccctcca gcgcctccag gaacccccag
     1141 atgtagtggc gccggcgctg ctcgccgtcc gtgacgatat ccaggcccgc gcttacctga
     1201 tcggcgatgg cgagccggac agcatcgtcg tgtgcctcgc cgagagcgtc gtccgcgacc
     1261 atccagtccg cgcgcagctg cgccgaaggg tcgctcagcc acttcggttt cggcaggctg
     1321 ccggtaatgc cactttctag agccgccatg ggcctttctc cgggaacttc gtgatcctag
     1381 cggtggtagg cctcgttgtt cggcgagtga tcgccgccga gccaacgggc gagctccgca
     1441 tgagccttgg cgcgctggtc cgcgttgatc tgatccacgt cctcgtgatc caccgtgaac
     1501 tcgatgatga gcttgctggg gtcgacgacg tagagcgagt agcagtagcc gtgattgagg
     1561 acgtaggtgt ccggctcctt gtagccggcc gccgccaggc ggtcgcgaat ggcgtcctgg
     1621 gtttcctgat ccaccttcat ggcgatgtgc cggaacggcg aggaaggcag ctcaggcccg
     1681 aactcggcgg catccgccgg atccgcgaac tggaagaacg cgagactctc accgccgccc
     1741 acgtcgaaga agcagtgcat gtaggtgcgc tccctgccga agagctcggc ctgctcacac
     1801 caggtggcga tcagcggaaa gccgatcacc tcctcgtaga acctgcgcac cgcttccatg
     1861 ttctcggtga cgtaggcgtt gtggtgcaga cgtgaattga ccggtggtgc gttgctcatg
     1921 atgtcctcgc tattgcccag ctcagaaccg cttggtgaaa tccacgcccc agcgcgccgg
     1981 cggggctttg aacaggaaac cgcccggaga gtactccgcg ttgtacttct tatcgaacgc
     2041 gttcttggcc caggccgtga gcgcccagtc tccgggcacc tcgacgccga gccgcaggtc
     2101 caccagatcc acggggctgc gcttggtgat gttggccggc tcccagtaag tatcgccaat
     2161 gcgctggtag tccacccggc ccaccagctc cagccgaccg ttcatggcgc ccaggggctg
     2221 gcggtactgc aggccgacgt tgagcgtgta ctcggatacc agcggtgcct ggttgccctc
     2281 gtggtcggta tttgccgcct cgtcgatctc gctgtccacg tacccgaagc ccaggtagg
//
LOCUS       DBNHOX010000616         1597 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_629182, whole genome shotgun sequence.
ACCESSION   DBNHOX010000616 DBNHOX010000000
VERSION     DBNHOX010000616.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_629182"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="AC16UZ_13515"
     CDS             complement(<1..598)
                     /locus_tag="AC16UZ_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIR6247749.1"
                     /translation="MHVKIGKTYNLCSLEPWMPSLNDELTFTRVDHRHGYIPDDEPRT
                     TFHQRRLYSVPENTPTVGCFPAGLLARVLEFLRARGHTWEIADHRDLARVKPAPDFTK
                     LDKLRPGQDKCILQIAYQDCGIIKAATAFGKSFIIKQCCKMYPNTRVLVTSPRVSVVN
                     TLYRNLVEALGEDQVGIVGGGHDVRGRRVTVSTTKSMHK"
     gene            complement(598..>1597)
                     /locus_tag="AC16UZ_13520"
     CDS             complement(598..>1597)
                     /locus_tag="AC16UZ_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247750.1"
                     /translation="MKEPGVVLDELKKPGADVGAFINTIGGVVAGMKLFPLPSMLANL
                     TQKWAVIHLVDGEVRMRTSDDYDALRTAAETVVGTCDLDQEHAFAVPGEIIALCMQND
                     EKVVSGFQVKGHTYFFMIKTPEFMAVYGCKPLQLEEPDVVDENATPEPENPGTPENEP
                     LQQEGESAPEEQAAAEEPEEQPAAEPMEQPEEIAGPCEAAPEGSTEEAGGEPVAEPAS
                     SQSPGSGEPAPKPQADDYDVQEALDVLLPDLVKGVKDLTHANSVEMAKILKNIAKQVR
                     KQMGRKADPSELAEIQAKLTEALKRAEAAEAVLDGVRGELEPMKSVLNIKTKGFWK"
BASE COUNT          241 a          498 c          496 g          362 t
ORIGIN      
        1 gtttgtgcat gctcttggtg gtcgacacgg tcacccggcg gccacggacg tcatgcccgc
       61 cgccgacgat tccgacctgg tcttcgccga gcgcctccac cagattccgg tagagcgtgt
      121 tgacgacgga gacccgcgga ctcgtgacca gcacccgcgt gttcgggtac atcttgcagc
      181 attgtttgat gatgaacgat ttgccgaagg cggtggccgc cttgatgatg ccgcagtcct
      241 ggtaggcgat ctgcagtatg cacttgtcct gcccaggccg cagcttgtcg agcttcgtga
      301 agtccggcgc cggtttgacg cgggccagat cccggtggtc ggcgatttcc caggtgtggc
      361 cgcgcgcccg gagaaactcc aggacgcggg ccaacagacc ggcaggaaaa caaccgacgg
      421 tgggggtgtt ctcggggacg ctgtacaatc gacgctgatg aaaggtcgtg cgcggttcgt
      481 cgtccgggat gtacccgtgc ctgtgatcca cccgggtgaa cgtgagctcg tcgttcaacg
      541 acggcatcca cggctcgagt gaacacaggt tgtaggtctt gccgatcttt acgtgcatta
      601 cttccagaag cccttggtct tgatgttgag caccgacttc atgggctcga gttcgccgcg
      661 aacgccgtcg aggacggcct ctgcggcctc ggcgcgcttg agcgcttcgg tcagcttcgc
      721 ctggatttcg gccagctcgg acggatcggc cttgcggccc atctgcttgc gaacctgctt
      781 ggcgatgttc ttcaggatct tggccatctc cacgctgttc gcgtgagtca ggtccttgac
      841 gcctttgacc agatccggca acagaacgtc cagagcttcc tgaacatcgt agtcatcggc
      901 ctggggcttc ggagcgggct ctccgcttcc ggggctctgg gagcttgcgg gctctgccac
      961 gggttccccg ccagcttcct ccgtcgatcc ttccggtgcc gcttcgcagg gtcctgcgat
     1021 ttcttctggc tgttccatcg gttcggcggc cggttgctcc tccggttctt ccgctgccgc
     1081 ctgttcctcg ggagcgcttt cgccctcctg ctgcaatggc tcattttcag gagttcctgg
     1141 attttcgggt tcgggggttg cgttctcgtc gaccacgtcg ggctcctcga gttgtagggg
     1201 cttgcagccg tagacggcca taaactccgg ggttttgatc atgaagaagt aggtgtggcc
     1261 cttgacctgg aagccgctga ccaccttctc gtcattctgc atacacaggg cgatgatttc
     1321 accgggaacc gcgaatgcgt gctcctgatc gagatcacag gttccgacga cggtttccgc
     1381 cgccgtgcgc aacgcgtcgt aatcgtcgct ggtacgcata cgaacctcgc cgtcgaccag
     1441 gtggatcacc gcccacttct gcgtgaggtt cgcaagcatc gacgggagcg ggaacagctt
     1501 catgccggcc acgactccgc cgatggtgtt gatgaaggca ccgacgtcgg cgcccggctt
     1561 cttcagttcg tcgagcacga cgcccggctc cttcatc
//
LOCUS       DBNHOX010000617         6326 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1335996, whole genome shotgun sequence.
ACCESSION   DBNHOX010000617 DBNHOX010000000
VERSION     DBNHOX010000617.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1335996"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /locus_tag="AC16UZ_13525"
     CDS             <1..1383
                     /locus_tag="AC16UZ_13525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR6247751.1"
                     /translation="KVVGGSSSINGMIYSRGHPEDYEEWMALGGPAWGWAEMKAAYRS
                     IEDHELGSADHRGAGGLVHVSTGKLRYPAAEAMIEAGREMGLERKDDLNDEMLEGVGY
                     YAHNIRRGRRQSAARTFLKAARRRPNLHVQSQAHVDRVCFDGNRAVAVACRVAGDRRV
                     FEARGEIILSAGTILSPKILQLSGIGPADHLRDHGIEVVRDVPDVGARMREHLGFSMP
                     HKLKGTKGLNHRLRGLGLVGSALQYVATRGGPLATGPFEVGAFVRAVPGANRPDTQLY
                     LGAFNYARSNDNFPVQLASPDSKPGMTIYGQLLNLTSEGTVRLRSADPDDPPVIVPNW
                     LSTDYDRAAAVAMVRYMRRYVRQPALARYVGDELIPGPTCQTDEEILNAVRRLSTSGL
                     HGVATCRMGRDEGAVVDGRLRVRGVEGLRVADCSVMPSLVSGNTNAPAMALGWRASDL
                     IEEDAAGSRR"
     gene            complement(1390..2619)
                     /locus_tag="AC16UZ_13530"
     CDS             complement(1390..2619)
                     /locus_tag="AC16UZ_13530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450 family protein"
                     /protein_id="HIR6247752.1"
                     /translation="MIDLNRPVNLASPEFAKDKYEWYERIREERPVFQTKISVLTVYT
                     VSRYEDCTNVLKDPNVLRNRTTATGGSRFPFPMPKSVQPLVQSMIQEDDPNHRRLREL
                     VRRAFRPQAIEQLESQIDAYSHELLDGLARRDSFDLQADYARPIPIRMIGDMMGLSRE
                     AVPQFQKAIRVLSEGFNGLKVLRTLFLDLPGAVRFVRALLDEKRSMPGDDVLTGLIEA
                     EDDGDRLTDDELVAMVFLLIVGGFETTLHLITNGVVTLLEHTEQLQRLKEDPGLIDSA
                     VEEILRHRGPVHGTKPGYAREDITLHGMTIPKGKPIMPLLAAANHDPRVFEKPQTFDI
                     ARSPNRHLGFGHGVHFCLGAHLARAEARVALRNLFERFPDLQLAVDPASLKLQAMPGW
                     HRYDGLPVIAGKMRSAA"
     gene            complement(2767..3297)
                     /locus_tag="AC16UZ_13535"
     CDS             complement(2767..3297)
                     /locus_tag="AC16UZ_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247753.1"
                     /translation="MAGNLGLIAGLILVGVQITDSNRIASAELFGSSVGSVISINTEQ
                     LGDSPGEAMSRVLYDPAAAETSDLYIADRIYDALLRQLVRVHVFDTMGLYGDDELVSP
                     AGFVGLHFEFFACPYGLAWLDQQIELFDTRGNEELAILTSLKMLRELAGAESARVRLE
                     DRLERVGALLAQLDDS"
     gene            3710..4564
                     /locus_tag="AC16UZ_13540"
     CDS             3710..4564
                     /locus_tag="AC16UZ_13540"
                     /inference="COORDINATES: protein motif:HMM:NF025679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HIR6247754.1"
                     /translation="MTVGLTDAPVDGVMAVHVEFRGITLKPQSGPAIDFALSEKVQLL
                     DLQNGSVFPLLDAQTVDAGRYNWIELHTNAEIDGILDSYVVESKSNGMVELEVPSGTV
                     RLVSGFVVTAGKNNAVLLDWDVRKGLTDPEGLVSPSPDAKDAYKLTPALRLIDMTQYG
                     SLTGSVADALIMAESCTSNAEGDGNLVYVYRNHDTTPDDLGSFGEPVTTAPVRSNPEK
                     AGAYTYTVHYLDPGDYTVAFTCQGLDDDPATDAAEGAAVPDIEFSGQVNASVAEGEGT
                     TNEAPPID"
     gene            4685..5059
                     /locus_tag="AC16UZ_13545"
     CDS             4685..5059
                     /locus_tag="AC16UZ_13545"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6247755.1"
                     /translation="MPDLAIGYVNVFVSDFDTALAFYRDKLGLEVAMADEEFGYASFD
                     TKGVGFAIAAVGQDAEPAFAPGRHTGIGWGVESLDDAYETLRSTGVEFTAPPERQPWG
                     GYMALFEDPDGNVFYLDELREH"
     gene            5078..6001
                     /locus_tag="AC16UZ_13550"
     CDS             5078..6001
                     /locus_tag="AC16UZ_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6247756.1"
                     /translation="MHRALGVDQLGCTLFVLVWAFIGANPWEENHMAFEAIRYEKTDG
                     VATITKNNPPQYGMTLAVLTEMRAALIDANNDADVCVVVITAGGEGFHMGAVVFGEAR
                     SDWKFSPLEFREVIQVGHDLFRLIETLEKPVVGVAKAGAVGGGFENLHACDFVIASEA
                     ARFSQPEATLGMICGWGGTQRVPRMVGWRKAKEILMEGVELTGREAAEIGLITRAVPL
                     DEVDEAVSALCERLKICGPVALGYTKLAMNKVWETDYRSGLDYEVEASGMVNSAGEFT
                     AEVFEDFLAGRQPKFAKRQRLTAGREWRGTD"
     gene            6147..>6326
                     /locus_tag="AC16UZ_13555"
     CDS             6147..>6326
                     /locus_tag="AC16UZ_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247757.1"
                     /translation="MSDDLLDNPAFAALQHAHAGLGRASELAAVYDPLVSPLAGLSEA
                     TPEALLDLAGLVPSGA"
BASE COUNT         1095 a         1938 c         2139 g         1154 t
ORIGIN      
        1 aaggtggtcg gcggttccag ctccatcaac ggcatgatct actcccgcgg gcatcccgag
       61 gactacgagg agtggatggc gcttggcggc ccggcgtggg gctgggcgga gatgaaggcg
      121 gcttatcgct ccatcgagga tcacgagctg gggtcagccg accaccgcgg agcgggtggg
      181 ctggtgcacg tcagcacggg caagctgcgc tatcccgcag ccgaggccat gatcgaggcc
      241 ggccgcgaaa tgggcctgga gcgcaaggac gacctcaacg acgagatgct cgagggtgtt
      301 ggctactatg ctcacaacat ccgccgcgga cgtcggcaga gcgcggcgcg cacttttctg
      361 aaggccgccc ggcggcgccc caacctgcac gtgcagtcgc aggcccacgt cgatcgggtg
      421 tgcttcgatg gcaatcgggc cgtggcggtg gcgtgtcgcg tcgcgggtga caggcgggtc
      481 tttgaggccc ggggcgagat catcctctcg gccggcacca tcctctctcc caagattctg
      541 cagctctccg gcatcggccc ggccgaccac ctgcgggatc acggcatcga agtggtgcgc
      601 gacgttcccg acgtgggcgc gcggatgcgc gagcatctgg gtttttcgat gccgcacaag
      661 cttaagggaa ccaagggcct gaaccatcgg ctgcgcggcc tgggtctggt gggcagcgcg
      721 ctgcagtacg tggcgacccg cggcggcccg ctcgccaccg gacccttcga ggtcggggcc
      781 ttcgtccgcg ccgtgcccgg cgccaaccgg ccggacacgc agctctacct cggcgccttc
      841 aactacgcgc gcagcaacga caactttcca gttcagctgg cgtcgcccga tagcaagccg
      901 ggcatgacca tctacggcca gctcctgaat ctgacaagcg aaggcacggt gcgtctgcgc
      961 tccgccgatc ctgacgaccc gccggtgatc gtgcccaact ggctcagcac ggattacgat
     1021 cgcgcggcgg cggtcgccat ggtccgttac atgcgccgct acgtgcgcca gccagcgctg
     1081 gccaggtacg tcggcgacga gctgatcccc gggccgacct gccagacgga cgaggagatc
     1141 ctcaacgccg tgcggcggct ttccaccagc ggcctgcacg gtgtggccac ctgccggatg
     1201 ggccgggacg aaggcgcggt ggtggacggc cggttgcggg tcaggggcgt cgaaggtttg
     1261 cgcgtcgccg attgctctgt gatgccctcc ctggtctcgg gcaacaccaa tgccccggcc
     1321 atggcgctgg gttggcgggc aagcgacctg atcgaagagg acgcggcagg ctcgcgtcgc
     1381 tgatcgcagc taggcggctg agcgcatttt cccggcaatc acgggcagcc cgtcgtagcg
     1441 gtgccagccc ggcatggcct gcagcttgag ggacgccgga tccacggcca gctgcaggtc
     1501 gggaaatcgc tcgaataggt tgcgcagcgc cacccgcgct tcggcccggg cgaggtgtgc
     1561 gcccaggcag aagtgcacgc catggccgaa gccgaggtga cgattgggtg agcgggcgat
     1621 gtcgaatgtc tgtggtttct cgaacacccg tgggtcgtga ttggcggcgg caagcagcgg
     1681 catgatgggc ttccccttcg gaatcgtcat gccatgaagc gtgatgtcct cccgggcgta
     1741 gcccggcttg gtgccgtgca cgggaccccg gtgtcggagg atctcctcca ccgccgaatc
     1801 gatcaggccg ggatcttcct tcagccgctg cagctgctcg gtgtgctcga ggagcgttac
     1861 gaccccgttg gtgatgagat gcagcgtcgt ctcgaaccca ccgacgatca acagaaatac
     1921 catggccacc agctcgtcgt cggtgagccg gtcgccatcg tcctcggctt cgatgagccc
     1981 ggtgaggacg tcgtcgcccg gcatcgagcg tttctcgtcg agcagggcgc ggacgaaacg
     2041 aacggcgccg ggcagatcca gaaacagcgt tcgcagcacc ttcagcccgt tgaagccctc
     2101 cgagagcact cggatcgcct tctgaaattg cggtaccgcc tcgcgggaaa gccccatcat
     2161 gtcgccgatc atgcggatgg ggatcggtcg cgcgtagtcg gcctgaagat cgaagctgtc
     2221 cctgcgcgcc agcccgtcga gcagttcgtg ggagtaggcg tcgatctggc tctccagctg
     2281 ctcgatggct tggggccgga aggcccgtct gaccagctcg cgcaggcgcc ggtgattcgg
     2341 gtcgtcttcc tggatcatgc tctgcacgag cggctggacg gacttcggca tggggaaggg
     2401 gaagcggctt cctccggtgg ccgtggtgcg gttgcgcaga acgttcgggt ccttgaggac
     2461 gttcgtgcag tcctcgtagc gggaaacggt gtagacggta agcacgctga tcttggtctg
     2521 gaacaccggg cgctcctcgc ggatgcgctc gtaccattcg tacttgtcct tcgcgaactc
     2581 cggcgaagcc agattcaccg ggcggttcag gtcgatcatg ggctggactc ctggcgcctc
     2641 gcagcgggtg ctcgtccgga tctatcggac cgctgttgcc cctttaccgg gcagacgttc
     2701 gtttatctgt tgcagagcat accaatacca aagggcgtgc gcccgcgagc ggccgtcgtt
     2761 atgacttcag ctgtcgtcca gctgcgccag cagggccccc acccgctcca ggcgatcctc
     2821 gagcctcacg cgcgcgcttt cggcgcccgc gagctcccgc agcatcttca ggctcgtgag
     2881 aattgccagc tcctcgttgc cgcgggtgtc aaacagctcg atttgctggt cgagccaggc
     2941 gaggccatag ggacaggcga agaactcgaa gtgaaggcct acgaaccccg cggggctgac
     3001 gagctcgtcg tcaccgtaaa gccccatggt gtcgaatacg tgcacacgga ccagctggcg
     3061 cagcagcgcg tcgtaaattc ggtcggcgat gtagaggtcc gaggtttccg cggcagcagg
     3121 gtcgtacagc acgcgtgaca tcgcctcacc aggtgagtcg ccgagctgct cggtgttgat
     3181 tgagatgacg gagccgacgc tgctaccgaa gagctccgct gaggcgatgc ggttcgagtc
     3241 ggtgatctga acgccgacca gaatcagccc ggcgatcagc cccagattgc ctgccacctg
     3301 caaaagctcc aacaactttg cgcgcgccac aatggttctc ccggagattg tgattggatc
     3361 gaggttagca gaagtcggcc gtttctcgtt tcgaccctgg cttgcgcggc tctgcaccgc
     3421 ttatcagcac cgcatgactt tctcggtcac agccacccga gcgtgaagcc gctaacgcct
     3481 cgggggtgaa ccatttggca gatcgacgca tgcgtcgcag ccgatcggcg acccgatgcg
     3541 tcgtacctgc ttaggtctcg ttcacgcgtg gaaacagccg cgtggtcgaa ggtcaacagg
     3601 agaaggagtt atgtctgcgt accgttcgat tgtctttgcg gcgttgacct ggtcaatgtt
     3661 gggatgtggg ggatccagcg gatccgatga gggtccatcc accggcacgt tgaccgtcgg
     3721 cctgacggat gcccccgtcg acggcgtgat ggcggttcac gttgaattcc gtggcatcac
     3781 gctcaagccc cagagcgggc ctgccatcga ctttgcgctc tccgagaagg ttcagctcct
     3841 cgacctgcag aacggcagcg tgtttccgtt gctggatgcc cagaccgtcg acgcgggccg
     3901 ctacaactgg atcgaactgc acaccaacgc ggagatcgac gggattctgg attcgtacgt
     3961 ggtggagagc aaatccaacg gcatggtcga gctggaggtg ccgagcggca cggtacgtct
     4021 ggtgagcggt tttgtggtga ccgccggcaa gaacaacgcg gtgcttctcg actgggacgt
     4081 ccgcaaaggg ctgaccgatc cggaggggct ggtgagcccg agtccagacg ccaaggatgc
     4141 gtacaagctc accccggcgc tgcgactgat tgacatgacc cagtacggca gcctgaccgg
     4201 cagcgtggcg gatgctttga tcatggctga gtcctgcacg agcaacgccg aaggcgacgg
     4261 caacctggtg tatgtttacc gaaatcacga cacgacgccc gatgatctcg gcagcttcgg
     4321 cgagcccgtg accacggcgc cggtccggtc caacccggag aaggcgggtg catataccta
     4381 cacggtgcac tacctggatc ctggcgacta cacggttgcc tttacctgtc agggactcga
     4441 tgacgatccc gccacggacg cggctgaagg cgccgccgtg ccggacatcg aattcagcgg
     4501 tcaggtcaac gcgtcggttg ctgagggcga agggacgacc aacgaagcgc cccctatcga
     4561 ttgatcgttt gaccgcagcg gcctagcgtg tggccgatcg cgccccggca ttggtcatgt
     4621 aagcgctcgc gcaccccgca gtggtggtat cgtatggtct tgtcgtggcg ttcgggagag
     4681 cagaatgcct gatctggcca tagggtacgt gaacgtcttt gtcagtgatt tcgatacggc
     4741 gctggccttc tatcgggaca agctgggtct cgaggtcgcg atggccgatg aggagttcgg
     4801 ctacgcgtcc ttcgatacca agggcgtcgg gttcgccatt gccgcggttg gccaggacgc
     4861 tgaaccggcc tttgctccgg ggcgacatac cgggatcggc tggggagttg aaagtctgga
     4921 cgatgcctac gaaacgcttc gcagcacagg ggtcgagttc acggctcccc ccgagcgaca
     4981 accgtggggc ggttacatgg cgttgttcga agatcccgac ggcaatgtct tctatctgga
     5041 cgagcttcgg gagcattgag ggccggctct ccttgcgttg catcgcgcgc tgggggtcga
     5101 ccagctcggc tgcacgctct tcgtgctggt ctgggcgttt ataggcgcaa acccctggga
     5161 ggaaaaccat atggcgttcg aggccattcg ctacgaaaag acggacggcg ttgccaccat
     5221 caccaagaac aacccgccgc agtacggcat gacgcttgcc gtgctgacgg agatgcgggc
     5281 tgcgctcatc gatgccaaca acgacgctga cgtttgtgtc gtggtcatca ctgcaggggg
     5341 cgagggcttt cacatggggg ccgtggtttt cggcgaggcg cgcagcgact ggaagttctc
     5401 ccccctggag tttcgcgagg tcatccaggt gggacatgat cttttccgcc tgatcgagac
     5461 gctggagaaa cccgtcgtgg gcgtggccaa ggcgggggct gtaggcggag gcttcgaaaa
     5521 tctccacgct tgcgatttcg tcatcgcctc cgaggccgcg cgcttcagcc agccggaggc
     5581 gacgctcgga atgatctgcg gctggggcgg tacccagagg gtcccgagaa tggtgggctg
     5641 gcgcaaggcg aaggagatcc tcatggaagg cgtggagctg accggcaggg aagccgcgga
     5701 gatcggcttg atcacccgtg cggttccgct cgacgaggtg gatgaggccg tctccgccct
     5761 ctgtgaaagg ttgaagatct gcggtccggt ggcgctcggc tacaccaagc ttgcgatgaa
     5821 caaggtctgg gaaacggact accgctcggg cctggactac gaggtagagg cgtccggtat
     5881 ggtcaacagc gccggggagt tcactgccga ggtgttcgaa gattttctcg ccgggcggca
     5941 gccgaaattc gcaaaacgcc agcggcttac cgctggacgg gaatggcggg ggactgacta
     6001 gcagcgcaca cctattgccg ggcgagggcc gttagacacg ccccctggct cgaactatct
     6061 tcggctcgat gggcaagaat gcagaaagtg agccgccgct ttgcacgtcg tggcagaggg
     6121 gccgcaccga atcggagctg gtgctgatgt cagacgatct gctcgacaat ccggctttcg
     6181 cggcgctgca acacgctcac gctggtcttg gtcgggcaag cgagctggct gccgtctacg
     6241 acccgctggt atcgccgctc gccggccttt ccgaagcgac gcctgaagcc ctgctcgacc
     6301 ttgccggtct ggtgccatcg ggcgcc
//
LOCUS       DBNHOX010000618         2693 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_179608, whole genome shotgun sequence.
ACCESSION   DBNHOX010000618 DBNHOX010000000
VERSION     DBNHOX010000618.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_179608"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /locus_tag="AC16UZ_13560"
     CDS             complement(<1..1412)
                     /locus_tag="AC16UZ_13560"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6247758.1"
                     /translation="MFARHRSLVDASGRIPSWRLGIFASIAFPSVGMTLPLAIFLPPF
                     YTKTLGLGLAEVGLVFMLVRLFDIVTDPVMGIIGDRFDSRWGRRRHWLVIALPFMMIG
                     VYMAFMPTGSPSIWYLGFWLVVLYAGTTMKTISHTAWAAELSPDYDQRSRIAGFNSFA
                     AYMGSLLILGPLAFLEFRGTPPAGHEALTFFGTMALVFAPICVFAAVTLVGERPTAPA
                     PRIGLIRGLRMVLRNPHMRRLLLADAMAAIPGSVMSGLFIFYQAELLGNSQFNSLALI
                     GFFVAHIIGVPLWVRLSFRVGKHRAFGISSLCFCFTTSLFFFPGEGDVALFVAFLFFT
                     GLTHSGLQFLLRSMGADVVDYDNVQTGGQRTGLYFALLSITAKAGGALAIGITYPLLA
                     VVGFDAQGGNSEDAKFAFRMIYLVVPVMAMILAYVFIRGFKLDEAEQRALQSRIAMRD
                     GSADGEAGAAGSADVDGDGQR"
     gene            complement(1421..>2693)
                     /locus_tag="AC16UZ_13565"
     CDS             complement(1421..>2693)
                     /locus_tag="AC16UZ_13565"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6247759.1"
                     /translation="DPRTCPVEGVMEVDFNSEPFLTDFKQTYAAMHAGGCPYAHSTAG
                     DHYAVASHAQIVAMLKRYDLWKSKHGPGLNDQERAGVLVSVDPPEHTFEVKIVASAFS
                     VPYFESLIPEMQAFVDEQIDGFHADGRVDLHQALSIALPLFVIFRMFGIPLRDEAGDK
                     TEFVREMIIGSVGLMLTPGHQVQAELAAGSVDEARMRAIAGTQQMFVDHLADCKRKLE
                     SGEYHDDSNIVCRFLTTPGPDGTYLSDAKILGFCGFLLTAGSATTTIMLSNVVYRLLR
                     QPEELAKLKANPELVPLAIEESLRLDAPVQGLFRTNDEETDLGPLHLEKDTKVMLLWA
                     GANLDPTVFDDPLRFSLDRDLAKVRRHLAFGYGTHFCRGAALARIEGEIFLRTVLERL
                     PNLRLDGEVVMEKRMPVLQGIRSLPLAWDVD"
BASE COUNT          539 a          875 c          882 g          397 t
ORIGIN      
        1 cgctggccgt ccccgtcgac gtcagccgat ccggccgccc ctgcttcccc gtccgccgat
       61 ccgtcacgca tcgcgatccg ggactgcagc gcccgctgct ccgcctcgtc gagcttgaat
      121 ccccgaatga agacgtaggc gaggatcatg gccatgacgg gcaccacgag gtagatcata
      181 cggaaagcga acttcgcgtc ctccgaattt ccaccctggg catcgaaacc caccaccgca
      241 agcagcggat aggtaatgcc gatggcgagc gccccgcccg ccttggcggt aatggagagc
      301 agcgcgaagt aaaggcccgt tcgctgcccg cccgtctgca cgttgtcgta gtccaccacg
      361 tccgccccca tggaccgcag cagaaactgc aggcccgaat gggtcaaacc cgtgaagaac
      421 agaaacgcca cgaacagcgc gacgtcgccc tcgcccggga agaagaacag cgacgtggtg
      481 aagcagaagc acagcgagct gatgccgaac gcgcggtgct tgccgactct gaacgacagg
      541 cgtacccaga gcggcacgcc gatgatgtga gccacgaaga agccgatgag cgcgagcgag
      601 ttgaactggg agttccccag cagctccgcc tgatagaaga tgaacagccc cgacatcacc
      661 gaaccgggaa tggctgccat ggcatcggca agcagcaggc ggcgcatgtg ggggttccgc
      721 agaaccatgc gcaggccgcg aatcagcccg atgcgaggcg ccggcgccgt ggggcgctcg
      781 ccaaccaggg tgaccgccgc aaacacgcag attggcgcga acaccagcgc catggtgccg
      841 aagaacgtca acgcctcgtg ccctgccggc ggcgttcccc gaaactcgag aaacgccagg
      901 gggcccagga tgagcagcga gcccatgtag gccgcaaacg agttgaagcc ggcgatgcgc
      961 gatcgctggt cgtaatcggg ggacagctcc gccgcccacg ccgtgtggga gatggtcttc
     1021 atggtcgtgc ctgcatagag caccaccagc cagaagccca gataccagat cgatggcgac
     1081 ccggtcggca tgaacgccat gtagacgccg atcatcatga agggcagcgc gatcaccagc
     1141 cagtgccggc gccggcccca gcggctgtcg aagcggtcgc cgatgatgcc catcaccggg
     1201 tcggtgacga tgtcgaacag gcgcacgagc atgaacacga gacccacctc ggcaaggccc
     1261 aggcccaggg tctttgtgta gaacggcggc aggaagatgg ccagcggcag cgtcatgccg
     1321 acgctgggga acgcgatgga ggcgaagatg cccagccgcc aggaggggat gcgcccggaa
     1381 gcgtcgacga gagaccgatg gcgggcgaac atgggccgcg tcagtccacg tcccacgcca
     1441 gcggcaggga gcggattccc tgcagcaccg gcatacgctt ctccatgacc acctccccgt
     1501 ccagcctcag attgggcagc cgctccagca ccgtgcgcag gaatatctca ccctcgatcc
     1561 gggcaagcgc cgcgccgcgg cagaagtggg ttccgtaccc gaaggccaga tggcggcgga
     1621 ccttggcgag atcccggtcc aggctgaaac gcagcgggtc gtcgaagacc gtcgggtcga
     1681 ggttggcgcc cgcccacagc agcatgacct tggtgtcctt ctccaggtgc agaggtccca
     1741 agtccgtttc ctcgtcgttg gtgcgaaaca ggccctgcac gggcgcgtcg aggcgcaggg
     1801 actcttcgat ggcaagcggc acgagctcgg ggttggcctt cagcttggcg agctcctcgg
     1861 gttgcctgag caggcggtag acgacgttgg acagcatgat cgtggtcgtc gcgctccccg
     1921 ccgtcagcag aaagccgcag aaaccgagga tcttcgcatc cgacaggtag gtgccgtccg
     1981 gacccggcgt ggtcagaaaa cggcagacga tgttgctgtc atcgtggtac tcgccggatt
     2041 cgagcttgcg tttgcagtcc gccagatggt ctacgaacat ctgctgggta ccggcaatgg
     2101 ctctcattcg ggcctcgtcc acggacccgg cggcgagctc ggcctgcacc tggtggccgg
     2161 gcgtcagcat cagcccgacg gagccgatga tcatctcgcg cacgaactcc gtcttgtcgc
     2221 cggcctcgtc gcgcagcggg atgccgaaca tccggaagat cacgaacagc ggcagcgcga
     2281 tggacagcgc ctgatgcaga tccacccgcc catcggcatg gaaaccgtcg atctgctcat
     2341 ccacgaacgc ctgcatctcg ggtatgaggg actcgaagta gggcaccgag aaagcgctgg
     2401 cgacgatctt cacctcgaag gtgtgctccg gcggatccac gctgacgagc acgcccgcgc
     2461 gctcctgatc gttgagaccg gggccgtgct tgctcttcca aagatcgtag cgcttgagca
     2521 tggcgacgat ctgggcatgg gacgccacgg cgtagtgatc tcccgcggtg gaatgcgcgt
     2581 aagggcagcc gcccgcgtgc atggcggcgt aggtctgctt gaagtcggtg aggaagggct
     2641 cggagttgaa atccacctcc atgacccctt ccaccggaca ggtcctggga tcg
//
LOCUS       DBNHOX010000619         7038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1014618, whole genome shotgun sequence.
ACCESSION   DBNHOX010000619 DBNHOX010000000
VERSION     DBNHOX010000619.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7038
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1014618"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="AC16UZ_13570"
     CDS             <1..479
                     /locus_tag="AC16UZ_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine amidotransferase-related protein"
                     /protein_id="HIR6247760.1"
                     /translation="GLDGAHSTEIDAETPHPVIALITEWLDRDGQIEQRTSETDLGGT
                     MRLGEQVCVLKDGTIAQRMYGAETVRERHRHRYEVNNQYIERLEAAGLVVAGRSEDGE
                     LVEMIELSGHPWFVACQFHPEFTSTPRAGHPLFSGFIGAASDRAAAAPVLRVGTSD"
     gene            495..1340
                     /gene="kdsA"
                     /locus_tag="AC16UZ_13575"
     CDS             495..1340
                     /gene="kdsA"
                     /locus_tag="AC16UZ_13575"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HIR6247761.1"
                     /translation="MRLCDFEVGLDRPLFLIAGPCVIESEQPALETAEALKALTDELA
                     VAFVYKSSFDKANRSSHESFRGPGLEAGLRILERVNREVGVPVLTDVHEDTPLDEVAA
                     VVSVLQTPAFLCRQTDFIQRVCSQGLPVNIKKGQFLAPADMIHVVTKARATGNEQILV
                     CERGASFGYNNLVSDMRGLAIMRATGCPVVFDATHSVQMPGGLGAASGGQREMVPVLA
                     RAAVAAGISGIFMETHPDPDQALSDGPNSWPLSLMAPLLRQLVDLDRVAKERPFLEDQ
                     YAQGR"
     gene            1344..2627
                     /gene="eno"
                     /locus_tag="AC16UZ_13580"
     CDS             1344..2627
                     /gene="eno"
                     /locus_tag="AC16UZ_13580"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR6247762.1"
                     /translation="MSRIAAVIGREILDSRGNPTVQADVVTDDGFMGSACAPSGASTG
                     SREALELRDGDASRYLGRGVLNAVGAVNGEVAKALAGVDPADQEAIDALLCELDGTPN
                     KGRLGANGLLAVSLAAAKAAAAAKNQALFEHINDLFGGVAMRMPLPMMNILNGGAHAD
                     NNVDIQEFMVQPVSMGSFREGLRCGAEVFHALGRLLRSRGLTTSVGDEGGFAPDLASN
                     AHALAVVAEAVSDAGSELGKDVTLALDCAATEFYADGQYRLAGEHRDLSSEEFAAYLE
                     DLTARYPVVSIEDGMDENDWDGWRALTDRVGDRIQLVGDDLFVTNTEILAEGIRRGVA
                     NAILIKLNQIGTLSETLSTIRMAQEAGYRTVISHRSGETEDTTIADLAVATGAGQIKT
                     GSLSRSDRVAKYNRLLRIEEMLGDAAEYRGRGELL"
     gene            2590..2913
                     /locus_tag="AC16UZ_13585"
     CDS             2590..2913
                     /locus_tag="AC16UZ_13585"
                     /inference="COORDINATES: protein motif:HMM:NF016839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HIR6247763.1"
                     /translation="MPLSTAVAASCSEPMATRLLIVVLAVLVALLQYRAWFGEVGYFA
                     AQELADEVDEQRRRGAELARRNELLTAEVVALKTGVDALEARARKDLGMIKRGETFYL
                     IPDDD"
     gene            2903..3868
                     /gene="truD"
                     /locus_tag="AC16UZ_13590"
     CDS             2903..3868
                     /gene="truD"
                     /locus_tag="AC16UZ_13590"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HIR6247764.1"
                     /translation="MTIDPHFGRLPLAVPGKRAKARVKATPEDFLVDELRAAAPSGAG
                     EHLYLHVEKRLLETRQVARRLAELYGVAAVDVGYAGMKDKRAVARQWLSVRTSLGPEV
                     AERGGLVVLAAGRDERKRRRGELAGNRFEIRLREVSGDDWRDRLGRVFESGAPNYFGP
                     QRFGGDNLDRALAWLPVRRRRSISPFRRGLYLSVLRSLLFNAVLARRVQEKTWRALLP
                     GDVDLDGMPTAPLWGRGRSATSGVALAAESAALAPFADVLDGLEHAGVDQARRSLVLL
                     PQNAWVAEEDEAVRLGFELPPGGYATSLLREAFELGEATDGAPAS"
     gene            3895..4530
                     /locus_tag="AC16UZ_13595"
     CDS             3895..4530
                     /locus_tag="AC16UZ_13595"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR6247765.1"
                     /translation="MTSQRTRDRLVQRLAEHGIVDQEILDLIGHVPRHIFVDEALSHR
                     AYEDSALPIGHGQTISQPFVVAFMTSTLLNAPRARVLEVGTGSGYQTAVLRSSRRVRR
                     VFTVERLGPLLARAEERLKAMGLESVRYRHGDGYEGWPEEAPFDGILVTAAPRQVPEA
                     LLDQLAEGGRMVVPVGGGGVQELRVYDRTATGVNVDTVRHVRFVPLVKGLL"
     gene            4541..5413
                     /locus_tag="AC16UZ_13600"
     CDS             4541..5413
                     /locus_tag="AC16UZ_13600"
                     /inference="COORDINATES: protein motif:HMM:NF015951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HIR6247766.1"
                     /translation="MSARGARQWSGLFARGMAMGVAEVVPGVSGGTIAFITGIYHELV
                     TTLAGFRPASARLLVTAGPVAFWQQHNLGFLAVLVAGMAASVLLFARLIGYWLDVVPT
                     LVWAFFFGLIAASVFQIGRGRPTRTLAVYGTIGLAAGASLLLVDPLPAQGAVWVFFFA
                     GMIAISAWLLPAISGSFILLILGLYEPVIEALNGFDWVVLTSLGLGCATGLMIFARAL
                     EWLLSYWREPLLSLLTGFMAGSLVRLWPWAQGGTLLTPAEYAAVTGQASLELWAVAAA
                     AAGALALWLLSRLE"
     gene            complement(5430..5753)
                     /gene="fdxA"
                     /locus_tag="AC16UZ_13605"
     CDS             complement(5430..5753)
                     /gene="fdxA"
                     /locus_tag="AC16UZ_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HIR6247767.1"
                     /translation="MTFVIGENCIKCKLTDCVEVCPVDCFYEGPNMLVIHPDECIDCA
                     LCEPECPVDAIYSEDELPEDQQDFLEINREQAELWPNITEKKDPAEDYEDWIGKPDKR
                     HLLER"
     gene            complement(5847..>7038)
                     /gene="mutS"
                     /locus_tag="AC16UZ_13610"
     CDS             complement(5847..>7038)
                     /gene="mutS"
                     /locus_tag="AC16UZ_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR6247768.1"
                     /translation="ATLKVGYNRVHGYYIETSRAAGNEVPAEYVRRQTLKNAERYITP
                     ELKQFEDEALTSQARALKLEKQLYQNLLEQLGARAGGLRDAAAILAELDVLSCFAERA
                     SALRLVRPEFSADPIIDIRQGRHPLVEQASAKPFVANDLSLGEKARMLIVTGPNMGGK
                     STYMRQSALIVLLAFAGSYVPAEAARLGPIDRIFTRIGAADDLAGGRSTFMVEMTETA
                     NILRNATPRSLVLLDEIGRGTSTYDGLALAWATAEHLARETGAFTLFATHYFELTELP
                     EELSQCRNVHLTAAEHDGKIVFLHSVEPGPASQSYGVQVARLAGVPNEVIARARTRLQ
                     SLEQQQAERIAQQPDLFEGQSAEPEAPSPLEARLSELDVDGLSPREALALLYELKGLA
                     SPDS"
BASE COUNT         1097 a         2193 c         2444 g         1304 t
ORIGIN      
        1 gcggattgga cggcgctcat tccaccgaga tagacgccga gacgcctcac ccggtcattg
       61 ccctgatcac cgaatggctg gatcgggacg gccagataga gcagcgcacc agcgaaaccg
      121 acctcggcgg cacgatgcgg cttggcgagc aggtgtgtgt tctcaaggac ggcaccatag
      181 cccagcggat gtacggcgcc gagaccgtcc gagaacggca ccgacaccgc tacgaagtga
      241 acaaccagta tatcgagcgg ctggaagccg ccggactggt ggtggcagga cgctcggaag
      301 acggcgagct ggtggaaatg atcgagctga gcggccatcc gtggttcgta gcctgccagt
      361 tccacccgga attcacgtcg accccgcggg ccggacaccc cctgttttcg ggcttcatcg
      421 gggcagcgag cgaccgcgcc gccgcggctc cggtgctgag agtcggcacg tccgactagc
      481 aaggaacccc cctcatgcgc ctctgcgatt tcgaggttgg tctggaccgg ccgctcttcc
      541 tcattgccgg tccctgcgtg atcgagtctg agcagccggc actggagacc gcggaggcgt
      601 tgaaagcgct caccgacgag ctagccgttg ccttcgtcta caaaagctcc tttgacaagg
      661 ccaaccgttc gtctcacgag agttttcgcg ggcccgggct cgaggccggg ttgcggattc
      721 tggaacgcgt caaccgcgaa gtgggcgtac ccgtgctgac cgacgttcac gaagacaccc
      781 cgctcgacga ggtggccgcc gtggtttcgg tgctgcagac gccggccttt ctctgccggc
      841 aaacagactt catacagcgg gtctgcagcc aggggctgcc cgttaacatc aagaagggtc
      901 agtttctcgc ccctgcggat atgattcacg tggtcacgaa ggcacgcgca acgggcaacg
      961 agcagatcct ggtctgcgag cggggcgcga gcttcggcta caacaacctg gtctcggaca
     1021 tgcgcgggct cgcaatcatg cgagccacgg gctgccccgt tgtcttcgac gcgacccact
     1081 cggtgcagat gccgggcggc ctgggcgctg cctcgggggg gcagcgggag atggttccgg
     1141 tgctggccag agcggcggtg gcggccggga tatcggggat attcatggag acccatcccg
     1201 atcccgatca agcgctatcc gacgggccca attcctggcc cctgtcgctg atggcgcccc
     1261 tgctccgaca gctggtggac ctggaccgcg tggcgaagga gaggccgttt ctcgaggacc
     1321 aatatgcaca aggaagatag gacatgagca gaattgcggc ggtaattggc cgcgagattc
     1381 tcgattcgcg gggtaacccc acggtgcagg ccgacgtcgt caccgacgac ggcttcatgg
     1441 gctccgcctg cgctccctcc ggggcgtcca cagggtccag ggaggcgctc gagctgcgcg
     1501 acggcgatgc atcccggtat cttggcaggg gcgtgctgaa cgcggttggg gccgtaaacg
     1561 gggaggttgc caaggcgttg gcaggcgtcg acccggccga ccaggaggcg atcgacgcac
     1621 tgctttgcga gctcgacggg acgccaaaca agggcaggct cggcgcaaac gggttgcttg
     1681 cggtgtccct ggcggccgcg aaagcggcgg cagcggccaa gaaccaggcg ctcttcgagc
     1741 acatcaacga tctcttcggt ggtgtcgcga tgcgcatgcc gctgcccatg atgaacatcc
     1801 tcaatggcgg cgcccatgcg gacaacaacg tggacatcca ggagttcatg gtgcagccgg
     1861 tctccatggg gtcgttccgc gaagggctgc gctgcggggc agaggtattt cacgccctgg
     1921 ggaggctgct gcgctcgcgg ggcctcacca cctcggtcgg ggacgaaggc ggcttcgctc
     1981 cggacctggc ctcgaacgcg catgctctgg ccgtggtcgc cgaagcagtg agcgacgccg
     2041 ggtccgagct cggaaaggac gtaacgctcg cgctggattg cgctgccacg gagttctacg
     2101 cggacggtca gtaccgtctg gccggcgagc atcgtgacct gtccagcgag gagttcgccg
     2161 cctatctcga agacctgact gcgcgctatc cggtggtctc catcgaggac ggcatggacg
     2221 agaacgactg ggacggctgg cgcgcgctga ccgatcgggt cggcgatcgg attcagctcg
     2281 tgggcgacga tctgttcgtc acgaacacgg agatccttgc cgagggcata cgccgggggg
     2341 tggcaaacgc gatcctgatc aagctcaacc agatcgggac tctgagcgaa accctcagca
     2401 cgatcagaat ggctcaagag gcaggatatc gcaccgtgat atcccaccgg tcgggtgaaa
     2461 cggaggacac caccattgcc gatctggccg ttgccaccgg cgcgggccag atcaagaccg
     2521 gctccctgag ccgttcggat cgggtggcaa aatacaaccg cctgctgcgc atcgaggaga
     2581 tgctcggcga tgccgctgag taccgcggtc gcggcgagct gctctgagcc catggcgacg
     2641 cggttgctca tcgtcgttct ggccgttctg gtcgccctcc tgcagtaccg ggcatggttc
     2701 ggcgaggttg gttacttcgc cgcgcaggag ctggcggacg aggtggacga gcagcgccgc
     2761 cggggtgcgg agctggcgcg gcgcaacgag ctgctgacgg cggaagtggt ggcgctcaag
     2821 acgggcgtag acgcgctcga ggcccgggca cgcaaggatc tgggcatgat caagcggggt
     2881 gaaacttttt atctgattcc ggatgacgat tgatccgcat ttcggccgtc tgcccttggc
     2941 cgtaccggga aagcgggcca aggcccgtgt caaggcgact ccggaagact tcctggtcga
     3001 cgagctgcga gccgcggcgc cgagcggcgc gggcgagcat ctctacctcc acgtggagaa
     3061 gcgcctcttg gaaacgcggc aggtggcccg tcgtctggcc gagctttacg gggtcgcggc
     3121 cgtggatgtg ggctatgccg gaatgaagga caagcgcgcc gttgcccgtc agtggttgag
     3181 tgtgcgcacg tctctcggcc ccgaggtggc ggaacggggc ggcctggtcg tgcttgccgc
     3241 aggtcgtgat gagcgcaagc gtcgtcgggg tgagctcgcc ggaaaccggt tcgagatccg
     3301 gttgcgcgag gtgtccggtg acgactggcg cgaccgcctc gggcgcgtct tcgagtcggg
     3361 agcgccgaac tacttcgggc cccagcgttt tggaggcgac aatctcgacc gcgccctagc
     3421 ctggcttccc gtacgccgcc gccggtccat ctcgccgttc aggaggggtc tctacctgtc
     3481 cgtgctccgc agcctgctct tcaacgcggt tctggcccgg cgggtgcagg agaaaacctg
     3541 gcgggcactg ctcccgggcg acgtggatct ggacggaatg cccacagccc ccctctgggg
     3601 ccggggtcgg tctgcgacca gcggcgtggc cctggcggcg gagagcgccg cgctcgcccc
     3661 gttcgcggat gtgctagacg gcctcgagca tgcgggggtg gatcaggcca ggcgatccct
     3721 ggtgctgttg ccgcagaacg cctgggtggc ggaggaagac gaggctgtgc gcctcggctt
     3781 cgaactgccc cctggcggat atgcgaccag cctgctgcgc gaggctttcg agctgggcga
     3841 ggccacggat ggagcaccgg cgtcgtgacg ggctacgatc tggtcggcat cggcatgacc
     3901 tctcagcgca cccgggatcg gctcgtgcag cgactggccg agcacggcat cgtcgatcag
     3961 gaaatactcg acctgatcgg ccacgtgccc cggcacatct tcgtggacga agcgctgtcc
     4021 caccgcgcgt acgaggattc ggccctgccc atcggccacg ggcagaccat ctcgcagccc
     4081 ttcgtcgtcg ccttcatgac ctccacgctg ctgaacgcgc cacgtgcgcg ggttctggaa
     4141 gtagggaccg ggtccggata ccagacggcc gtgctgcgat cgagccggcg cgttcgccgg
     4201 gtcttcaccg tcgagcgtct cgggccgctg ctcgcccgcg ccgaagagcg cttgaaggcc
     4261 atggggctgg aatcggtcag gtaccgccat ggcgacggtt acgaaggatg gccggaggaa
     4321 gcaccgttcg acggcattct cgtgacggca gcaccgcggc aggttccgga agctctgctg
     4381 gatcagctgg cggaaggcgg gaggatggtg gtgcccgtgg gcggcggtgg cgtgcaggaa
     4441 ctgcgcgttt acgatcgcac tgccaccggc gtcaacgtgg acacggtgag gcacgtacgc
     4501 ttcgtgccgc tggtcaaggg cctgctatga gggtcgcagc gtgagcgccc gcggcgcccg
     4561 tcagtggagc ggtctcttcg cgcgcggcat ggccatgggc gtcgccgagg tggttcccgg
     4621 tgtgtccgga ggcaccattg ccttcatcac gggcatctac cacgagctcg tgaccaccct
     4681 ggccgggttc aggcccgctt ccgcgcgtct gctcgtgacc gcaggaccgg ttgcgttctg
     4741 gcagcagcac aatctggggt ttctggccgt gctggtggcg ggtatggctg ccagcgttct
     4801 gctcttcgcc cggctcatcg gctactggct cgacgtggtg ccgaccctgg tctgggcttt
     4861 cttcttcggg ctcatcgccg cttcggtgtt ccagatcggc cgcggtcgcc cgacgcgtac
     4921 gcttgccgtg tacggcacca tcgggctcgc tgccggggca tcgctcctgc tcgtcgaccc
     4981 gttgcccgcc caaggggccg tttgggtctt cttctttgcc gggatgatcg ccatctctgc
     5041 atggctgctc cccgctatct cgggtagctt catcctgctg attctcgggc tctatgagcc
     5101 cgtcatcgag gcgctgaacg ggttcgactg ggtggtgctc acgagtctgg gtctcggatg
     5161 tgccaccggg ctgatgatct tcgcccgggc gctggagtgg ctgctctcct actggcgcga
     5221 gccgttgctt tctctactga ctggattcat ggcgggctcg ctggtgcgcc tgtggccctg
     5281 ggcccaaggc ggcaccctgc tgacgccggc cgagtatgct gcggtgacgg gtcaggcgag
     5341 tctggaactg tgggcggttg ctgcggccgc tgcgggcgca ttggccctct ggctgttgtc
     5401 caggctggag tagacgcagg ccgaatcggt cagcgttcga ggagatggcg cttgtcgggt
     5461 ttgccgatcc agtcctcgta gtcttccgca ggatccttct tctcggtgat gttcggccac
     5521 agctcggcct gctcgcggtt gatctccagg aagtcctgct gatcctcggg aagctcgtct
     5581 tccgagtaaa tggcatccac cgggcattcg ggctcgcaaa gggcgcagtc gatgcattcg
     5641 tccggatgga tgacgagcat gtttggccct tcgtagaagc agtctaccgg gcatacctca
     5701 acgcagtctg tgagcttgca cttgatgcag ttttccccaa tgacgaacgt catctgtatt
     5761 gccttcctgt cgggtaagtt ggtgtttcgg gtctgcgaag ggaccgtatt ctaaccatat
     5821 tcgatcccct gtcgagcggg cgcccgtcag ctatcggggg acgccaaacc cttgagttcg
     5881 tagagcagcg cgagggcttc ccgagggctc aacccgtcta cgtccagctc ggacagcctc
     5941 gcttcgaggg gactcggcgc ctcgggttcc gcggattgtc cctcgaagag atcgggttgc
     6001 tgcgcgatac gctctgcctg ctgttgctcc agcgactgca ggcgagttcg cgcgcgggcg
     6061 atgacctcgt tgggcacgcc ggcaagtcgg gccacctgca caccgtagct ctggctggcg
     6121 ggccctggct cgacgctgtg caggaagacg atcttcccgt cgtgctcggc agcggtcaga
     6181 tgaacgttgc ggcactggga aagctcctcg ggcagctccg tcagctcgaa gtagtgcgtt
     6241 gcgaacaggg tgaacgcgcc cgtttcccgg gccagatgct cggcggttgc ccaggccagt
     6301 gccagcccgt catacgtgct ggtcccgcga ccgatctcgt cgagcaggac gaggctgcgc
     6361 ggcgtggcgt ttctcagaat gttcgcggtt tcggtcatct ccaccatgaa ggtagagcga
     6421 ccgccggcga ggtcgtcggc cgcgccgatg cgggtgaaga tgcggtcgat ggggcctagc
     6481 cgcgcggctt ctgccgggac gtagctgccg gcgaaagcga gcagcacgat gagggccgat
     6541 tgcctcatgt acgtagactt gccgcccatg ttgggccccg tcacgatgag catgcgtgcc
     6601 ttctcaccca gcgagaggtc gttggctacg aagggctttg cgctcgcctg ctcgacgagc
     6661 ggatggcggc cctgcctgat gtcgatgatg gggtccgccg agaactcggg tcgcaccagc
     6721 ctcagggcgc ttgcgcgctc ggcgaagcac gagagcacgt ccagctccgc gaggatggcc
     6781 gcggcatcgc gcaaaccgcc ggctcttgcg cccagctgct ccagcaggtt ctggtacagc
     6841 tgcttttcaa gcttgagcgc tcgtgcctgg ctggttagcg cttcgtcctc gaactgtttg
     6901 agctccgggg taatgtagcg ctcggcgttt ttcagggtct gtctgcggac gtactcggcc
     6961 ggaacctcgt tgccggccgc gcggctcgtc tcgatgtagt agccgtggac ccggttgtag
     7021 ccgaccttca gggtcgcg
//
LOCUS       DBNHOX010000620         2711 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1270649, whole genome shotgun sequence.
ACCESSION   DBNHOX010000620 DBNHOX010000000
VERSION     DBNHOX010000620.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1270649"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..257
                     /locus_tag="AC16UZ_13615"
     CDS             24..257
                     /locus_tag="AC16UZ_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247769.1"
                     /translation="MATNHEAGSSNLSGRAISITETGSDRKTMSSAPMNKDNRRFHGW
                     RLIGVIMIVIIALAAVSFVVDWMVIGPLEGRVF"
     gene            complement(268..834)
                     /locus_tag="AC16UZ_13620"
     CDS             complement(268..834)
                     /locus_tag="AC16UZ_13620"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6247770.1"
                     /translation="MRLSKLNRDDLTADQRAVLDAIQSGPRKSKHGAIGLVGPFGVWV
                     RAPRVGLATQALGAAARFDSRLPENVKEVAICTVGAFFRAKFEFAAHRELAVAAGVDP
                     EEIEKLRVGELPSFNGAEDLSYRVATSLLNLHRLDSNTYAGAIGQFGEEGLIELVTII
                     GYYCMVALTLNAFEVPLTSGMDDPFPDA"
     gene            1345..2403
                     /locus_tag="AC16UZ_13625"
     CDS             1345..2403
                     /locus_tag="AC16UZ_13625"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6247771.1"
                     /translation="MIDAQGMTVMPGFIDTHRHLLLYSNAGSARQLRRYINRTLSREL
                     SQTLAQGITTALSPGDHVPEIIEIRERIASGEMPGPRLLVAGPVIQARGDHVATTVCR
                     GRSFCRKRATAEARTPEEAREHVRRAASLGVDFIKTVHDRQVAPRVVASDELLAAIAD
                     ESAALKIPLLIHVRDAADMARLSALKPAAFVHAPLTGELATAIEAAAMSLWTVPVSTT
                     LAWQTPELFKARRARFHADDHERLEQALDNIRHLVSTNSVVAFGTDNPPPLGTDDLMT
                     EVRTLSRVLTPAEIIRSMTEHAAEFLGRGDELGTLAPGKLADLVVVEGDPLTDLEALS
                     RVRLVIKGGSVVADMRTR"
     gene            2474..>2711
                     /locus_tag="AC16UZ_13630"
     CDS             2474..>2711
                     /locus_tag="AC16UZ_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6247772.1"
                     /translation="MRHGIIFSGGGTSMQQMVDLACQAEQAGVDSIYLTEAWRSGFVG
                     LAAIAAATERVEIGPYILNAYARSVWVTAMSAIDL"
BASE COUNT          495 a          898 c          820 g          498 t
ORIGIN      
        1 cgtagctcag ttggatagag cgcatggcta cgaaccatga ggccgggagt tcgaatctct
       61 ccgggcgcgc catttccatc accgaaaccg gttccgaccg gaagacgatg agctctgccc
      121 cgatgaacaa ggacaacagg cgatttcacg gctggcggct catcggcgtc atcatgatcg
      181 tcataattgc cctcgcggcg gtcagcttcg tcgtggactg gatggtcatc ggtccgctgg
      241 aaggccgcgt cttctgagct tgcgcggcta agcgtccgga aacgggtcgt ccattccgga
      301 ggtcaaaggc acttcgaaag cgttcagggt cagggcaacc atgcagtagt agccgatgat
      361 ggtgacgagc tcgatgagcc cctcctcacc gaactggccg atcgccccgg cgtaggtgtt
      421 cgaatccagc cgatgcagat tcagcaggct cgtcgccacc cggtaggaca gatcctcggc
      481 cccgttgaac gagggcagct cccccacccg aagcttctcg atctcctcgg ggtcgacacc
      541 tgctgcgacg gcgagctccc tgtgcgcggc aaactcgaat tttgcgcgaa agaatgctcc
      601 aacggtgcag atcgccacct ccttcacgtt ctccggaaga cggctgtcga atcgtgccgc
      661 tgcacccagg gcctgagttg cgagccccac gcgcggcgcc cggacccaga cgccaaacgg
      721 accaacgagc ccgatggctc cgtgtttgct cttgcgcggc ccgctctgaa tggcatcgag
      781 cacggcccgt tgatccgcgg tcaggtcgtc ccgattcagt ttcgacaatc tcatgaggca
      841 cctccccttt ctccgggatc atcttcgttc aaacgccgtg aaatatcgaa tggccccagg
      901 cgcgggagcc gcgactttca acacgtaaca attggttacc ttatctccgt tgactgccat
      961 ggtgggggtg cctatggtcc tgcccgcagc gtcgcttcac ggcttcgcac caagcccgaa
     1021 accacagcta acggaccgcc aagtgatgat gcacagcaac gagactgccc gcgacgactc
     1081 cgtctttgag aatgtcgttg gagtcatggc ggccgtgtct gtgctgtgct tcatggggtt
     1141 gttgctgttc atctgacggc cgcactactc ctcatatcac tcctaccgct ggccggatgg
     1201 gccagtcccg attccgccct gatcatcacc aatgcccgca tcctcgacgg tgcgggcagc
     1261 gtcatagaca acggcaccgt gcacgttctg ggtgagcgaa ttgcggccgt tggggccggg
     1321 ccatcaagca gcgacgcatc gatcatgatc gacgcgcaag gcatgaccgt catgcccggc
     1381 ttcatcgaca cccatcggca tctgctgctt tacagcaacg caggcagtgc gcgacagctg
     1441 cgtcgctaca tcaaccgaac cctttcccgc gaactctcgc agacgctggc tcagggaata
     1501 accaccgccc tctcgcccgg cgaccacgtt cccgagatca ttgagatcag ggagcgcatc
     1561 gcgtccggtg agatgcccgg gccccgcctg ctcgtggctg gccccgtgat tcaggctcgc
     1621 ggcgaccacg tggccaccac ggtatgccga ggccgctcgt tctgccgcaa gcgcgccacc
     1681 gccgaggcga gaacgcccga agaggcccgc gagcacgttc gccgcgctgc ctctctgggc
     1741 gtagacttca tcaagacggt gcacgaccgt caggtggcgc cccgcgtggt cgcgtcagac
     1801 gagctgctcg ccgccatcgc agatgaatcc gcggcgctta agattcccct gctcatccac
     1861 gtccgcgatg ctgccgacat ggcgcggctg tctgcattga aaccagcggc ttttgttcac
     1921 gcgcccctga ccggagagct ggccacggcc atcgaggcgg cagcgatgag cctctggacg
     1981 gtgcccgtat ccaccaccct cgcctggcag actccagagc tcttcaaggc gcgccgcgcc
     2041 cgttttcacg ccgacgacca tgagcgtctg gagcaggctc tcgacaacat cagacatctg
     2101 gtcagcacga actccgtggt ggcgttcgga accgataatc ccccgcccct gggaaccgat
     2161 gacctcatga ccgaggtgcg cacgctgagc cgcgtgctga cccccgcgga gatcatccgt
     2221 tccatgaccg agcacgcggc ggagtttctg ggtcgcggcg acgagctggg cacgctggcg
     2281 ccggggaagc ttgccgacct cgtggtcgtc gaaggcgacc cgctcaccga tctcgaggcg
     2341 ctgtcgcgag tgcgtctggt gatcaaaggc ggatcggtgg tcgcggacat gcgcacgaga
     2401 tgacgccggc ggacccttgg gcagaactcg tttcctggtg taaggtcccc tgagggacta
     2461 aagggagaga accatgcgtc acggcatcat tttttccggc ggcggcacca gcatgcagca
     2521 gatggtggat ctcgcctgcc aggcggagca ggccggtgtc gattcgatct acctgacgga
     2581 agcctggaga agcggcttcg taggactcgc cgccatcgct gctgccaccg agcgggtcga
     2641 gatcggcccg tacatactga atgcctatgc caggtccgtc tgggtcaccg ccatgagcgc
     2701 catcgatctg g
//
LOCUS       DBNHOX010000621         7027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1078684, whole genome shotgun sequence.
ACCESSION   DBNHOX010000621 DBNHOX010000000
VERSION     DBNHOX010000621.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1078684"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="AC16UZ_13635"
     CDS             <1..347
                     /locus_tag="AC16UZ_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6247773.1"
                     /translation="GGQKQRLAIARALLKRPEILLLDEATSALDAESEQHIRASITEL
                     KGQCSIVVIAHRLSTVREADRILVLSEGRLQASGRHEELLKSSELYARFSRIQFAGED
                     EADGSGARTTAG"
     gene            415..1548
                     /locus_tag="AC16UZ_13640"
     CDS             415..1548
                     /locus_tag="AC16UZ_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6247774.1"
                     /translation="MGPLHGFRIVELAGIGPGPFCGMMLSDMGAEVIRVDRISGGANR
                     PKDVLARNRRSIAVDLKSPAGAETVLRIVEKSDALFEGFRPGVTERLGLGPEDCWARN
                     EKLVYGRMTGWGQEGPLAQAAGHDINYIGLSGALHAIGHAGDRPVPPLNLVGDFGGGG
                     MLLAYGLVCGLLEAQKSGKGQVVDAAMVDGAAALMAMFFSMGAAGVFKDQRGTNLLDS
                     GSHFYDTYETKDGKYVCIGSIEPQFYALLVEKAGLDAERFAAQMDASRWPDYKRELTE
                     VFKSKTRDEWCEIMEGSDVCFAPVLSIFEAPDHPHSQARNAFIELDGVVQPAPAPRFS
                     RTEVAASHGARVPGEDSVSVLEDCGFTGDEIAGLKNDGVIGGN"
     gene            1564..2100
                     /locus_tag="AC16UZ_13645"
     CDS             1564..2100
                     /locus_tag="AC16UZ_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247775.1"
                     /translation="MAVELLDTERFHGFRVRRQIAGKTYQEYFSLKDDGKRIKGPQRA
                     TVKALAEARDAELANLQKAARERADRTVRVDARGQVRGILCRLKTEKSGTLTPVFQIG
                     VMSRLEDKIVNTTVSISRYGETEAWRRAIDFYALHKGISKRSRAYRDLLAAQPSGAKI
                     KSLLKASGRAAPRRSARR"
     gene            2097..3983
                     /locus_tag="AC16UZ_13650"
     CDS             2097..3983
                     /locus_tag="AC16UZ_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016562180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6247776.1"
                     /translation="MSYASEYARSLSDPEGFWREKAAELPWLEFPQTILDRAPSGAWR
                     WFHGGKTNTCWLALDRHVADGRGAQVALIYDSPATGSVQRFTFAELTERVAACAGGLA
                     ALGVSLGDRVIVYMPMIPEAAIAMLACARIGAIHSVVFGGFAAPELATRIDDAEPKVV
                     LSASCGIEFANVIPYQPLVDEAISLAVHKPERVVMLQREQQEADLSGERYLDWASWEA
                     SAQGVGCVPLDATDPLYVLYTSGTTGKPKGVVRDNGGHAVALNYSMEAIYGMDAGDVF
                     WAASDVGWVVGHSYIVYGPLIRGCTSVLFEGKPVRTPDAGVFWRVIQDHRVKCFFVAP
                     TAFRAIRKEDPDCALKDRYDVSSLKYQFVAGERLDPPTYHWLSEHLHVPVIDHWWQTE
                     TGWAMCANMAGLELKDAKPGSPTLPVPGYDLRILDEDGVEVPAGVEGAVVVREPLPPG
                     AFPTVWGDHQRYVDSYWSRYPGFYLTGDEGYRDDEGYVYIMGRMDDVINVAGHRLSTG
                     QMEETVAEHPAVAECAVVGIADADKGQVPVGLVLVKDGVNVDPQALEAELVQMVRASV
                     GAFANFRRAVVVERLPKTRSGKILRQVLRKMIDGNPYSVPSTIDDPAIIPEMASTLKK
                     AGVM"
     gene            4015..4350
                     /locus_tag="AC16UZ_13655"
     CDS             4015..4350
                     /locus_tag="AC16UZ_13655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6247777.1"
                     /translation="MIIVHGTIPIRPDQRERALELARRMSEATQAELGCISYDFYVGL
                     SDPNTLMLFQEWETMEALMAHFQTEHMDDFLRELPQVVAGEITTRRYAVQSVDEDTEE
                     TPETPPIVH"
     gene            complement(4344..4694)
                     /locus_tag="AC16UZ_13660"
     CDS             complement(4344..4694)
                     /locus_tag="AC16UZ_13660"
                     /inference="COORDINATES: protein motif:HMM:NF021369.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HIR6247778.1"
                     /translation="MKGLQLACLLMMGSLFAINGLRQFFVEPLATPHVNLIWFLIQVL
                     PLLAVLPGTLRNRYRSYVFAALVSTLYFIHGVLLAATADLRALGLWETGFAVGLLIAA
                     SYAARRTVGQAASQ"
     gene            complement(4691..5299)
                     /gene="wrbA"
                     /locus_tag="AC16UZ_13665"
     CDS             complement(4691..5299)
                     /gene="wrbA"
                     /locus_tag="AC16UZ_13665"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HIR6247779.1"
                     /translation="MTGPVHPYVLILYYSRTGGTAHLARYVARGVQAQGDFEARVRTV
                     PPVSTTVEQTEPVVPEHGAVYCTKAALAGCSALAIGSPTRFGNMAAPLKHFLDDTADL
                     WMQHALVGKPAGVFCSTASLHGGQESTLLSMMIPLLHHGMLVSGVPYSEPALNRTARG
                     GGPYGPSHVSGGEAGTEPTADEQAIAMALGRRLAELARRLGA"
     gene            complement(5303..5653)
                     /gene="arsC"
                     /locus_tag="AC16UZ_13670"
     CDS             complement(5303..5653)
                     /gene="arsC"
                     /locus_tag="AC16UZ_13670"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252402.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HIR6247780.1"
                     /translation="MREVTIYHNPRCSKSRATLALLEEHGVTPNVVEYLSTPPDAATL
                     KRLLDLLGLSPRELMRRKETAYSDLGLDDDALGEDALIEAMIANPILIERPIVVAGDR
                     AVIGRPPENVLGLV"
     gene            5716..6945
                     /locus_tag="AC16UZ_13675"
     CDS             5716..6945
                     /locus_tag="AC16UZ_13675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HIR6247781.1"
                     /translation="MLEATYDQFQERVVVPLRWYVGQLGQQFLIHDCMTRAGALTYTT
                     LFAVVPMMTVAYAMFSVLPEFETVGERIQEYIFQNFVPDSSAVVQERLLEFSARARQL
                     TAAGFLVLFITAFMMLVSVEKTFNTIWSVAEPRRGLQRFLLYWGVLSLGPPLIAAGIF
                     ISLYLISLPLIADLDTFGLDSAVLDYLPSVLTAAGLTVLFLAVPNCRVPFHHALAGGV
                     LTMFAFELAKKVFRLTVANSSMEPIYGTFAAVPLFLIWLYLVWVLILSGAIFVRTLSL
                     KRDRPVDNPEPAVVKCSRVVKFLHAAHLEGRSVSDSEINDQVPLARAERERVFEALLA
                     LKLVRETEDGGWMLGRSLKTLTLWDLYRALPDGVDAARLGTVKDMDGVVEPLKAFVQF
                     GSNQMAVSLDSVFGKSS"
BASE COUNT         1210 a         2180 c         2374 g         1263 t
ORIGIN      
        1 ccggagggca gaagcagcgg ctggccatcg cccgcgccct tctgaaacga ccggagatcc
       61 tgctgctcga tgaggcgacc agcgcgcttg atgccgagag cgagcagcac atccgggcga
      121 gcatcaccga actcaagggc caatgcagca tcgtcgtcat cgcccaccgt ctgtccacgg
      181 tccgcgaggc ggatcggata ctcgtgctga gcgagggacg gctccaggcc agcgggcgcc
      241 acgaggagct tctcaaaagc agtgagctgt atgcccgttt ctcgcgtatt cagttcgccg
      301 gcgaggacga agcggacggc tcgggtgcta gaaccaccgc gggatgatgc ccacgcaaaa
      361 gatcgctaaa ctcctcggct gatagtctga tttcttaccg gggggaaggt gagcatgggg
      421 ccgttacacg ggtttcgtat tgttgagctg gctgggatcg gtcccggacc tttctgcggg
      481 atgatgctgt ccgacatggg cgccgaggtg atccgggtgg accggatcag cggcggggcc
      541 aaccgcccga aggacgtgct tgcccgcaat cgacgctcca tcgccgtgga cctgaagagc
      601 ccggcgggcg cggaaaccgt gctgcgcatc gtcgagaaat cggatgcgct gttcgagggt
      661 ttccgcccgg gcgtgaccga acggctcggc cttggtcccg aggattgctg ggcgcgcaac
      721 gagaagctcg tttacgggcg catgacgggc tggggccagg aagggccgct ggctcaggcg
      781 gccgggcacg acatcaacta catcggtctg tcaggggctc ttcacgcgat cggtcacgcg
      841 ggggaccgcc cggtgccgcc gctgaacctc gtcggcgatt tcggaggcgg cgggatgctg
      901 ctcgcctacg gcctggtctg cggcctgctc gaagcccaga aatccggcaa ggggcaggtg
      961 gtagatgcgg ccatggtgga cggggcggcg gccctgatgg ccatgttctt ctccatgggc
     1021 gctgccgggg tgttcaagga ccagcgcggc accaacctgc tcgacagcgg ctcgcacttc
     1081 tacgacacct acgagaccaa ggacgggaag tacgtctgca tcggctccat cgagccccag
     1141 ttctacgccc tgctggtcga gaaagcaggc ctggacgcgg aacgctttgc cgcccagatg
     1201 gacgcctcca gatggccgga ctacaagcgt gagctgaccg aggtgttcaa aagcaagacg
     1261 cgcgacgagt ggtgcgaaat catggaaggc agcgatgtct gcttcgctcc cgtgctgtcc
     1321 attttcgaag cccccgacca tcctcacagc caggcccgca acgccttcat cgagctggac
     1381 ggcgtcgtcc agcccgcgcc ggcccctcgc ttctcccgaa ccgaggtggc ggccagccac
     1441 ggtgcccggg tacccggcga ggactcggta tcggtgctgg aggactgcgg atttacagga
     1501 gacgagatcg ccgggctcaa gaatgacggc gtgatcggcg gcaactgacc ggaggatcgg
     1561 cagatggcgg tagagttgct ggacacggag cgttttcacg gattccgtgt gcgcaggcag
     1621 atcgctggga agacctatca ggagtatttc tctctcaagg acgacggcaa gcgcatcaaa
     1681 ggtccgcagc gggccacggt caaagccctg gccgaagccc gcgatgccga actcgcgaat
     1741 ctgcagaaag cggcgcggga aagagcggac aggacggtgc gcgtagatgc cagaggccag
     1801 gttcgcggaa tcctgtgtcg gctcaagacc gagaagagcg gtaccctgac accggtattt
     1861 cagatcggcg tcatgtcgcg cctggaagac aagatagtca acacgaccgt gtcgatcagc
     1921 cgctacggcg agacggaggc ctggcgccgc gccatcgact tctacgcgct gcacaaaggg
     1981 atcagcaaac gatcccgcgc ctatcgtgac ctgctggcag cgcaaccgag cggcgcgaag
     2041 atcaagagcc tgctcaaggc gtccgggcgc gccgcaccgc ggcgatccgc ccgaagatga
     2101 gctacgcgtc agagtacgcg cgatcgctct ctgatccaga agggttctgg cgggaaaaag
     2161 cggcagagct tccctggctc gagtttccgc agaccatact ggaccgcgcg ccgtcgggcg
     2221 cctggcgctg gtttcacggc ggcaagacga acacctgctg gctggccctg gacaggcacg
     2281 tggcggacgg ccgcggggct caggtcgcgc tcatctacga ttcgcccgcg accggcagtg
     2341 tccagcggtt cactttcgca gagctcacgg agcgggtggc cgcctgcgcg ggtgggcttg
     2401 cggccctcgg cgtgtccctg ggcgatcgtg tcatcgtcta catgcccatg attcccgagg
     2461 cggcgattgc gatgctggcc tgtgcgcgga tcggtgccat tcactctgtg gttttcggcg
     2521 ggtttgccgc gcctgagctc gctacccgga tcgatgatgc cgagcctaag gtggttctga
     2581 gcgcgtcgtg cgggatcgag ttcgccaacg tcatcccgta tcaacctctc gtagacgaag
     2641 ccatttccct tgccgtccac aaaccggaac gcgtggtgat gctgcagcgc gagcagcaag
     2701 aagcggatct ttccggcgag cgttatctgg actgggccag ctgggaggcc tcggcccagg
     2761 gtgtcggctg cgtgccgctc gatgccaccg atccgctcta cgtcctttac acgtcgggca
     2821 ccaccggaaa gcccaagggt gtggtgcggg acaacggcgg ccacgcggtg gcgctgaact
     2881 acagcatgga agccatctac ggcatggacg cgggcgacgt tttctgggcc gcgtcggacg
     2941 tcggctgggt ggtgggccat tcctacatcg tctacggacc cctcatccgt ggctgcacct
     3001 cggtcctgtt cgaagggaag cccgtgcgga cccccgacgc gggcgttttc tggcgcgtga
     3061 ttcaggatca ccgggtgaag tgcttcttcg tcgcgccaac ggcctttcgc gcgattcgca
     3121 aggaggaccc cgactgcgcg ctgaaggatc gttacgacgt ctcctccctg aagtaccagt
     3181 tcgtcgccgg cgagcgcctc gacccgccca cctatcactg gctgagcgag cacctgcacg
     3241 tccctgtgat cgaccactgg tggcagacgg aaacgggctg ggcaatgtgt gcgaacatgg
     3301 ccggtctgga gctcaaggac gcgaaaccgg gctcgccgac cctccccgtt ccggggtacg
     3361 acctgcgcat tctcgatgaa gacggcgtcg aggtgcctgc tggcgtggaa ggggcggtcg
     3421 tggtcaggga gcccctgccg cccggcgctt tcccaacggt ttggggcgat catcagcgct
     3481 acgtcgattc ctactggtcc cgctatcccg gcttctacct gaccggcgac gagggctatc
     3541 gcgacgacga aggttacgtc tacatcatgg gccgcatgga cgacgtcatc aacgtagccg
     3601 gccaccggct ctccaccggg cagatggagg agacggtcgc cgagcatccc gcggtggccg
     3661 agtgcgccgt ggtcggcatt gcggatgcgg acaaaggcca ggttcccgtc ggtctggtgc
     3721 tggtcaagga cggtgtcaac gtcgatcctc aagcgcttga agccgagctc gtgcagatgg
     3781 ttcgggcatc ggtgggcgct tttgccaact tccggcgggc ggtggtcgtg gagcgtctgc
     3841 ccaagacgcg ctcgggaaag atcctgcggc aggtgctgcg aaagatgatc gatggcaatc
     3901 cctacagcgt tccctccacc atcgacgatc ctgcgatcat cccggagatg gcctcgactt
     3961 tgaagaaagc gggggtgatg tgaccgggct gctgctaaca tcggcaacag gcagatgatc
     4021 atcgttcacg gcaccatccc aatccgtccg gatcagcgcg aacgcgctct ggagctcgcc
     4081 cgccggatga gcgaggcaac ccaggcagag ctcggctgca tctcctacga cttctacgtg
     4141 gggctgtccg atcccaacac gctgatgctt tttcaggaat gggagaccat ggaagcgctg
     4201 atggctcact tccagaccga gcacatggac gacttcctcc gagaacttcc gcaagtggtg
     4261 gcgggggaga tcacgacccg gcgctatgcc gtgcagagcg ttgacgaaga caccgaggag
     4321 acgccagaga cgccgcccat cgttcactga gacgcagctt gccctaccgt gcgccgggcg
     4381 gcgtagctcg ccgcgatgag caggccgacg gcaaagcccg tttcccacag gcccagcgcc
     4441 ctgaggtcgg ccgtggcggc cagcagcacg ccgtgtatga agtaaagggt gctcaccagc
     4501 gcggcgaaga cgtagcttcg gtagcggttc ctcagcgtgc ctggaagcac ggccagcagg
     4561 ggcagcacct ggatcagaaa ccagatcagg ttcacatggg gcgtggccag cggctccacg
     4621 aagaactggc gcagcccgtt gatcgcgaag aggctgccca tcatcagcag gcacgcgagc
     4681 tgcagtcctt tcacgcgccg agcctacggg cgagctcggc caacctgcgc cccagggcca
     4741 tggcgattgc ctgctcgtcc gccgttggct cggtccccgc ctcgccaccg ctgacgtggc
     4801 tggggccgta tggcccaccg cccctggcgg tccggttgag ggcgggctcg ctgtaaggca
     4861 cgccggagac caacatgccg tggtggagca gcgggatcat catcgacagc agggtggatt
     4921 cctggccgcc gtgcagagag gcggtcgagc agaacacgcc agccggtttg cccaccaggg
     4981 cgtgctgcat ccacagatcg gccgtatcgt cgaggaagtg cttcagcggt gccgccatgt
     5041 tgccgaatcg ggtgggggag ccgatggcca gggcgctgca tccggccagg gccgccttcg
     5101 tgcagtaaac ggcgccatgc tcgggcacca cgggctcggt ctgctccacc gtggtggata
     5161 cgggcggcac ggtgcgcacc cgcgcctcga agtcaccctg ggcctgaacg ccgcgcgcga
     5221 cgtatcgggc caggtgcgcg gttccgccgg tacggctgta gtagaggatc aggacgtagg
     5281 ggtgcacggg tccggtcatg ggtcagacca gccccaggac gttctcggga ggccggccga
     5341 tgacggccct gtcgccggcg acgacgatgg gccgctcgat gagtatcggg ttggcgatca
     5401 tcgcctcgat gagggcatcc tcgccgaggg cgtcgtcgtc cagaccgaga tcggagtagg
     5461 cggtttcctt ccggcgcatg agctctcgcg gggagagtcc gagcaggtcg agcagccgtt
     5521 tgagggtcgc cgcgtcgggc ggcgtgctca ggtactcgac gacgttgggc gtcaccccgt
     5581 gctcttcgag cagggccagc gtggcccgcg acttggagca gcgcggattg tggtagatgg
     5641 tgacttcacg catggtcttt ataatgttcg gttctggcgg cgatttcatt taaccggtcg
     5701 cgcgggagac tggcaatgtt ggaggcgacg tatgatcagt tccaggaacg ggtggtcgtg
     5761 ccgctgcgct ggtacgtcgg gcagctgggt cagcagttcc tgatccacga ctgcatgacg
     5821 cgggcgggag cgttgaccta caccacgctc ttcgccgtgg tgccgatgat gacggtggcg
     5881 tacgccatgt tttcagtgct gccggagttc gagaccgtcg gcgagcggat ccaggaatac
     5941 atatttcaga acttcgtgcc ggacagcagc gccgtggtcc aggaacgcct cctggaattc
     6001 tcggcaaggg cgcgccagct caccgcggcg ggctttctgg tgctgttcat cacggctttc
     6061 atgatgctgg tctccgtgga gaagacgttc aacaccatct ggagcgtagc cgagccgcgg
     6121 cggggactgc agcgattcct gctgtactgg ggcgttctct ccctgggccc gccgctgatc
     6181 gctgccggga ttttcatttc tctgtacctc atatcccttc ctctgatcgc cgatctcgac
     6241 actttcgggc tggacagcgc cgtcctcgat tacctgccga gcgtgctgac cgcggcgggg
     6301 ctgaccgttc tcttcctcgc ggttcccaac tgccgggttc ccttccacca tgccctcgcc
     6361 ggcggcgtcc tgacgatgtt cgccttcgag ctggcgaaga aggtattcag gctcacggtt
     6421 gccaactcca gcatggagcc catctacgga acctttgctg cggtgcccct tttcctgatc
     6481 tggctctacc tcgtctgggt gctgatcctc agcggggcga ttttcgtccg caccctgtcg
     6541 ctcaagcgcg accggcccgt ggacaacccc gagcccgcgg tggtcaagtg ttcgcgggtc
     6601 gtgaagtttc tccacgctgc ccatctcgag ggccgaagcg tcagcgactc ggagatcaac
     6661 gaccaggtgc cgctggcccg ggccgaacgg gagcgggtat tcgaagcgct gctcgccctc
     6721 aagctggtga gggaaacgga ggatgggggt tggatgcttg gtcgcagcct gaagacgctg
     6781 acgctttggg atctctaccg tgcgcttccg gacggcgtgg acgcagcacg gctcggtacg
     6841 gtgaaggaca tggatggcgt ggttgaaccg ctcaaggcct tcgtccagtt cggctccaac
     6901 cagatggcgg tgagcctcga ttcggtgttt ggtaaatcct catgaaggcg aagacgagct
     6961 ctctcttgct ggctggtgcg cttttcctgg ccggctgtga ttttctcggc caggcagacg
     7021 agccgcc
//
LOCUS       DBNHOX010000622         2684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_564356, whole genome shotgun sequence.
ACCESSION   DBNHOX010000622 DBNHOX010000000
VERSION     DBNHOX010000622.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_564356"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /gene="lipB"
                     /locus_tag="AC16UZ_13680"
     CDS             complement(<1..609)
                     /gene="lipB"
                     /locus_tag="AC16UZ_13680"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR6247782.1"
                     /translation="MADVPVIVRQLGRTRYEPVFRAMQVFTEERAEPTADELWFTEHD
                     PVFTQGQAGKAEHLLAPGDIPVVQTDRGGQVTYHGPGQIVAYLLFDLRRMGLSVRGLV
                     SGIEEAVVRLLADYDIRSAPRRDAPGVYVAGSKIAALGLRVRRGYSYHGLALNVQMDL
                     EPFSRINPCGLFGMEVTQLADLGGPADVQTVEDELLTKLADVY"
     gene            complement(611..874)
                     /locus_tag="AC16UZ_13685"
     CDS             complement(611..874)
                     /locus_tag="AC16UZ_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP0495 family protein"
                     /protein_id="HIR6247783.1"
                     /translation="MTEPPKIEFPCDYPIRVIAENHLALRETVVRIVRLHAPDLAEST
                     VSVRNSREGRYCSVRFSITARGEPQLRALHRALMDEPMVKMVL"
     gene            complement(867..2054)
                     /locus_tag="AC16UZ_13690"
     CDS             complement(867..2054)
                     /locus_tag="AC16UZ_13690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR6247784.1"
                     /translation="MLRGLLMSFWANHLAAFCLGLAAVAGPAHAGEGPIIPPPPKIAG
                     TSYLLVDADTMKVLVENEIHEPVPPASLTKIMTGYIAAVELEAGRISLEDQVPISVKA
                     WRAPGSRMFVREGTRVGLEDLLRGIIVQSGNDASIAMAEHIAGSEDAFADMMDQQAIQ
                     LGMTTTQYQNATGLPSDDHYTSAWDLAVLTQSLIKRFPDSYKIYSERSFAFNDIEQPN
                     RNRLLWRDRTVDGVKTGHTSAAGYCLVASAKRGNMRLISVVLGTESEEARMRESQKLL
                     SYGFRYFETQKLYDAGVPLKTSKIWYGEADDIQLGLAEEVFVTIPRGHYEDLKAELSV
                     VKTIEAPVAAGQELGELRLHFGDELMLSAPLVALQEVKEADMFSRLLDGTRLLFRDLF
                     GVD"
     gene            complement(2082..>2684)
                     /locus_tag="AC16UZ_13695"
     CDS             complement(2082..>2684)
                     /locus_tag="AC16UZ_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HIR6247785.1"
                     /translation="EPKSARGNPPSYTVMGRTYRVMDSAAGYESTGIASWYGRKFHGR
                     ATSSGETFDMFELTAAHTSLPIPTYARVTNLHNGRSTIVRINDRGPFHADRLIDLSYA
                     AAMKLGFATRGTARVRVEALVDSPDYFLQAGAFSDMSSADALKNALGALTGARAYVVR
                     VSSDALFRVRIGPVKSRPEALRLQALIASSDFDRPLILEQ"
BASE COUNT          493 a          871 c          840 g          480 t
ORIGIN      
        1 gtagacgtcg gccagcttcg tcaggagctc atcctcgacg gtctgcacgt ccgccggacc
       61 gcccaggtcg gcaagctgcg tcacctccat gccgaacaac ccgcacgggt tgatgcgcga
      121 gaacggctct aagtccatct gcacattgag agccagtccg tggtaggagt acccgcgccg
      181 aactctcagc ccgagagccg cgatcttaga cccggccacg tagacccccg gcgcatcacg
      241 gcgcggcgcg ctgcggatat cgtagtcggc cagcagcctg acgacggctt cctcgatgcc
      301 gctgaccagg ccgcgcacgc tcagccccat gcgacgcagg tcgaacagca gataggcgac
      361 gatctggccc gggccgtgat aggtcacctg cccgccccgg tcagtttgca ccaccggaat
      421 gtcgcccggc gccaggaggt gctcggcctt gcccgcctga ccctgggtga acaccggatc
      481 gtgctcggtg aaccagagct catcggcggt gggctccgcc cgttcctcgg tgaagacctg
      541 catggctcga aataccggct cgtaacgggt ccgccccaac tgcctgacga ttacggggac
      601 atcagccacc tcacagcacc atcttgacca tgggttcgtc catgagagcc cgatgcaggg
      661 cccgcagctg gggttccccc ctggcggtta tcgagaagcg caccgagcag tagcggcctt
      721 cgcggctgtt cctgaccgat acggtgctct ccgccaggtc cggggcatgc aggcgaacga
      781 tccgcaccac ggtctcgcgg agcgcgaggt gattctccgc gatcacccgg atggggtagt
      841 cgcagggaaa ctcgattttc gggggttcag tcaacgccga aaaggtcgcg aaagaggagc
      901 cgggtgccgt ccagcaggcg cgagaacatg tctgcttcct tgacttcctg caacgcgacc
      961 aggggcgcgc tcaacatcag ctcgtcaccg aagtgcaaac gcagttcccc cagctcctga
     1021 ccggcagcta cgggagcctc gatggtcttc accacgctga gctcggcctt gaggtcttcg
     1081 tagtggcccc gggggatggt tacgaaaacc tcctctgcaa ggcccagctg gatgtcgtcc
     1141 gcctcgccgt accagatctt cgaggtcttc aaagggacac cggcatcgta gagtttctgc
     1201 gtctcgaaat agcgaaatcc gtaggaaagc agcttctggc tctcgcgcat cctggcctct
     1261 tcgctttccg tgcccagcac gacggaaatc aatcgcatgt tgccgcgctt ggccgaggcg
     1321 accaggcagt agccggcggc tgaggtgtgc ccggtcttga ccccgtccac ggtgcggtcc
     1381 cgccagagca gccggttgcg attgggctgc tcgatgtcgt tgaaggcgaa agatcgctcg
     1441 gagtagatct tgtagctgtc gggaaaccgc ttgatgagcg actgggtgag caccgccaga
     1501 tcccaggcgg aggtgtaatg atcgtcgctg ggaaggcccg tggcgttctg atactgggtc
     1561 gtggtcatgc cgagctggat cgcctgctga tccatcatgt cagcgaaagc atcttcactc
     1621 ccggcaatgt gctcggccat ggcgatgctg gcatcgttgc ccgactgtac gatgatgcca
     1681 cgaagcagat cttccaaccc gacccgggtg ccctcccgca cgaacatccg cgacccgggc
     1741 gcacgccacg ctttgacgct gatgggcacc tgatcttcca ggctgatccg gcctgcctcc
     1801 agctctacgg cggcgatgta gccggtcatg atcttggtca ggctggccgg tggcacgggc
     1861 tcatgtattt cgttttccac cagcaccttc atggtgtccg catctaccag gaggtaagag
     1921 gtgccggcga tcttcggcgg cggcggaatg atgggcccct cccccgcgtg ggcgggaccg
     1981 gcaacagccg cgaggcccag acagaaggct gcgagatgat tggcccagaa cgacatgaga
     2041 agacctctca gcatgctcgt acgcttccgg tggattctag ctcattgctc gagtatgagc
     2101 gggcgatcga agtcagaaga cgcgatcagc gcctgcaaac gaagcgcttc cggcctggac
     2161 ttgaccggcc cgatacggac ccggaacaga gcatcgctgg aaaccctcac cacataggcc
     2221 cgcgctccgg tcagcgcgcc gagggcattc ttgagcgcat cagccgagct catgtcgctg
     2281 aaagcaccag cctgcagaaa gtagtcgggg ctatccacca aagcctccac gcgcacccgc
     2341 gccgtgcccc gggtcgcgaa gcccagcttc atggcggccg cgtaggaaag gtcgatgagc
     2401 cgatcggcgt gaaacggacc gcgatcattg atccgaacga tagtggaacg cccgttgtgc
     2461 agattggtca cccgggcgta ggtgggaatc ggcagcgaag tgtgagcggc cgtcagctcg
     2521 aacatgtcga acgtctcgcc gctcgacgtc gctcgtccat ggaacttgcg gccgtaccag
     2581 ctggcgattc ccgtggactc atagcccgcc gcgctgtcca tcacccggta ggtgcgcccc
     2641 atcaccgtgt agctcggcgg gttgccgcgg gcgcttttgg gctc
//
LOCUS       DBNHOX010000623         2117 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_435796, whole genome shotgun sequence.
ACCESSION   DBNHOX010000623 DBNHOX010000000
VERSION     DBNHOX010000623.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_435796"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="AC16UZ_13700"
     CDS             complement(<1..495)
                     /locus_tag="AC16UZ_13700"
                     /inference="COORDINATES: protein motif:HMM:NF022616.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2955 domain-containing protein"
                     /protein_id="HIR6247786.1"
                     /translation="MPVQARRVFRLSGVMAVSLALSYAMQMDLPFIAPIFALLLTAAP
                     GPPKGLKALVGLSLVLAVTLGVGLLLVPLLLNYPVTAILIAGAGIYFSNYVSVNLGKG
                     AVGALLTAGVTLISAAGTLSFPLAVTVIEALVLGVGIAVISQWIVYPWFPENESFVPP
                     APPAA"
     gene            complement(513..1652)
                     /locus_tag="AC16UZ_13705"
     CDS             complement(513..1652)
                     /locus_tag="AC16UZ_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008041547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR6247787.1"
                     /translation="MSEQDHESEKAAEAQPESATAEKAVKRGGQMIGLVIVVTLVWYL
                     LADRYTPYTTQARVQGYVIGVAPKVSGLVTQVWAQNNREVQQGERLFEIDRSEYQIAV
                     GKARSDLENARRQVEAGRAAVEASRANLAAALANRVRAEKDYDRLQRLHSRDPGTIST
                     RRLEMSRASLDQAVAAVSAAESNVQAAIEQMGGEDEASNAILQSAQSAVARAELDLSN
                     TIVTASSRGVITDLRADVGQFAGAGSAVMTLIAVHDLWISAEFTENNLGHMRSGTEVE
                     ILFDVVPGRVFSGRIRSLGLGVSAGQSHPPGTLPTIQNNRDWLRQSQRFAVIVDFDPN
                     QHGELRSQLRIGGQASVMGLTEGHGVLNLLGQLYMRIMSWLSYAY"
     gene            complement(1649..>2117)
                     /locus_tag="AC16UZ_13710"
     CDS             complement(1649..>2117)
                     /locus_tag="AC16UZ_13710"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR6247788.1"
                     /translation="NVRVQDARLQQAIAGFQNTVLQAAREIDDAAITLVKTRERQATL
                     ADAVLSARRALDLARVRYREGYSDFQRVLDAQRALFGAAERDLVNKGNHISAVIQFYK
                     ALGGGWTEMPIGELLPETVREKMRTRSNWGDLLDAPLPVNQEGPVPAEGGTTP"
BASE COUNT          380 a          712 c          655 g          370 t
ORIGIN      
        1 cgcagccggc ggtgccggag gtacgaacga ctcgttttcc ggaaaccagg ggtagacgat
       61 ccactggctg atgactgcaa tgccaacacc caacaccagc gcttcgatca cggtcaccgc
      121 cagcggaaaa ctcagcgtac ccgccgccga tatcagggtg acgcccgccg tcaaaagcgc
      181 cccgacggca cccttgccca gattgacgct cacatagttg ctgaagtaga tgcccgctcc
      241 cgcgatcagg atggcggtga cgggatagtt gagcagcaag ggaacgagca gcagcccgac
      301 gcccagcgta actgccagca ccagggaaag cccgaccagg gctttcaggc ccttgggcgg
      361 gcccggagcc gccgtgagca gcagcgcaaa aatcggcgcg atgaacggca gatccatctg
      421 catggcgtag ctgagcgcca gagagacggc catcacgccg ctcagccgga agactcgtcg
      481 agcctggacc ggcatttcag aggccttccc gatcagtaag cataggacag ccagctcatg
      541 atgcgcatgt agagctgacc cagcaggttc agcaccccgt gcccttcagt gaggcccatc
      601 accgacgcct ggcccccgat gcgcagctgc gagcgaagtt cgccgtgctg gttcggatcg
      661 aagtccacga tgacggcaaa gcgctgggac tgtctcagcc agtcccggtt gttctgaatg
      721 gtaggcagcg tccccggcgg atgagactgg ccggcgctca ctccgaggcc gaggctgcgt
      781 atcctgccgg aaaagactct gcccggcacc acgtcgaaga ggatctccac ctcggtgccg
      841 cttcgcatat gccccaggtt gttctccgtg aactcggcgc tgatccaaag gtcgtgcacc
      901 gcgatgagcg tcatgaccgc gctgcccgca ccggcgaact ggccgacgtc cgcgcgaaga
      961 tccgtgatca cgccgcggga agaagccgtg acgatggtat tggacagatc gagctccgcg
     1021 cgcgcgaccg cgctctgtgc ggattgcaga atggcgttgg aggcttcgtc ctcgcccccc
     1081 atctgctcga tggcagcttg gacattcgac tcggccgccg agaccgcagc taccgcctgg
     1141 tcgagcgagg ctctggacat ttccagccgc cgggtggaga tcgtcccggg atcccgggag
     1201 tgaagtcgct gaagacgatc gtagtccttc tccgccctga cccgattggc gagggcagcg
     1261 gcgaggttgg cgcgagaagc ctctaccgcg gcgcgtccgg cctctacctg tctgcgggcg
     1321 ttttccaggt ccgagcgcgc cttgcccacg gcaatctggt actcagagcg gtcgatctcg
     1381 aacagtcgtt ccccttgctg gacctcgcga ttgttctgcg cccacacctg ggtcacgagg
     1441 ccggacacct tcggtgccac cccgatgacg tagccctgca cccttgcctg ggtggtatat
     1501 ggcgtgtagc ggtctgccag gaggtaccag acgagggtga cgacgatcac cagaccgatc
     1561 atttgaccgc cacgcttgac ggctttttcg gccgttgccg attccggttg agcttccgcg
     1621 gccttctccg actcgtgatc ttgctcgctc atggggttgt ccctccttcg gccggcacag
     1681 ggccctcctg attcacgggc agcggcgcat cgagcaggtc accccaatta ctccgagtgc
     1741 gcatcttctc ccgaacggtc tcgggcagca gctcaccgat gggcatctcc gtccaaccac
     1801 ccccaagggc cttgtagaac tgaatgacgg cgctgatgtg gtttcccttg ttgaccagat
     1861 cccgctcagc ggccccgaac agcgcccgct gggcatccag cacgcgctgg aagtcgctgt
     1921 agccctcgcg gtagcgcacc cgggccagat cgagagcccg ccgcgctgac agcacggcgt
     1981 ccgccagcgt cgcctgccgc tccctggtct tcacgagcgt gatggccgca tcgtctatct
     2041 cgcgggccgc ctgaagcacg gtgttctgga agccggcgat tgcctgctgc agccgggcat
     2101 cctgaacacg aacgttg
//
LOCUS       DBNHOX010000624         2168 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_49713, whole genome shotgun sequence.
ACCESSION   DBNHOX010000624 DBNHOX010000000
VERSION     DBNHOX010000624.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_49713"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="AC16UZ_13715"
     CDS             <1..1107
                     /locus_tag="AC16UZ_13715"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6247789.1"
                     /translation="GRGQIPTGSSKVELEPDVKLWLERCAERGWTAPTWPTEYGGGGH
                     SAGETKIIYEEMAEIGARAPLMGMGLSMIGPTLLEFGTPDQKERHVPSITRGEVHWCQ
                     GYSEPNAGSDLAALQTRAEDRGDHFVINGQKIWTSGATLADWMFALVRTDFDAPKHEG
                     ISFVLLTMDQPGVTIKPIRLISGNSPFCETFFDDAVASKADLVGELNKGWSVGKRLLQ
                     FERSGIGGLGGANARANPGSGLAEMARRFAGGAPGRIQDPDVRQAVTRFNMNRAAFRL
                     TTQRASEENRSGTPGAATSIFKLYGAALQQDAAELRRVFLGTQGLGADEGAFSDDELE
                     TTREWLATRATTIYGGTNEVQANIIAKRVLGLPD"
     gene            complement(1149..2147)
                     /locus_tag="AC16UZ_13720"
     CDS             complement(1149..2147)
                     /locus_tag="AC16UZ_13720"
                     /inference="COORDINATES: protein motif:HMM:NF048461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR6247790.1"
                     /translation="MPFNEFHSLPPGLLECEARELEGALGLPSLIHLPGGREPALFVS
                     VLLHGNEPSGWNGLRRLLADHPELPRSMSIFVGNVQAARAGMRALPGQQDFNRVWRGA
                     GGVEGEMARGVAGRLAERRFLAAVDLHNNTGHNPHYSVLSDLSVENLGMAYLFCDKAV
                     LIEEPDTVITHLFAGRCPAVTLELGPVSDPECEIRAYDYLLRCLRLDAVPAGHAEDFS
                     LYRSEARVHVPEGVSFHFADEGGAAPLVLTGGVEGVNFHELPAGFRFGSSEGRLEDVL
                     RVLDGAHQDVTERYFALQDGAILLKRPVVPAMYTTDPYVVHQDCLCYLMQRLEHAP"
BASE COUNT          432 a          713 c          712 g          311 t
ORIGIN      
        1 gggcgcgggc agatccccac gggcagcagc aaggtggagc tcgaaccgga cgtgaagctg
       61 tggctggagc gctgcgccga gcgcggctgg acggcgccga cctggccgac cgagtacggc
      121 ggcggaggcc actctgcggg cgagacgaag atcatctacg aggaaatggc cgagatcggt
      181 gcgcgggcac cgctgatggg catggggctg tccatgatcg ggccgacgct gctggagttc
      241 ggaaccccgg atcagaaaga aagacacgtt ccatcgatca cgcgcggcga ggtgcactgg
      301 tgtcaggggt actctgagcc gaatgccggc tccgacctgg ccgccctgca gacccgggca
      361 gaagaccgag gggatcattt cgtgatcaac gggcagaaga tctggacttc cggagcaacg
      421 ctggccgact ggatgtttgc cctggtgcgc accgacttcg atgcccctaa gcacgaaggt
      481 atcagcttcg ttctcctgac catggatcag cccggcgtga ccataaagcc catccgcctg
      541 atctccggga actcgccatt ctgcgagacc ttcttcgacg acgccgtggc cagcaaggcg
      601 gatctggtcg gcgaactgaa caaaggctgg tcggtgggca aaaggctgct ccagttcgag
      661 cgatccggca tcggcggcct tggcggcgcc aatgcccggg ccaaccccgg cagcggcctg
      721 gcagagatgg ctcgacgttt cgccggcggc gcgcccggca ggatccagga cccggatgtg
      781 cgccaggctg tcacccgttt caacatgaac cgggccgcgt tccggctcac cacccagcgc
      841 gcctcggagg agaacagatc cggcacgccc ggcgcggcta catccatctt caagctgtac
      901 ggggccgcgc tgcagcagga cgcggcggag ctgcgccgcg tgttcctcgg cacccagggt
      961 ctgggagccg acgagggagc attctcagac gacgagctgg agacgacgcg cgaatggctg
     1021 gccaccaggg caacgacgat ctacggcggg accaacgagg tgcaggccaa catcatcgca
     1081 aaacgcgtgc tggggctccc ggactgacga gcagcccgcc gtttgccggc aatggcggct
     1141 gaagcccgtc acggcgcgtg ctctaggcgt tgcatcaggt agcaaaggca atcctgatgc
     1201 acgacgtaag gatcggtcgt gtacatggca gggaccaccg gccgcttcaa caagatggcg
     1261 ccgtcttgca gagcgaaata acgctccgtc acgtcctggt gagcgccgtc caggacacgc
     1321 agcacgtcct ctaggcggcc ttcggagctg ccaaaccgaa atcccgcggg aagctcgtgg
     1381 aaattgaccc cctccacgcc tccggtcaga accagcggcg cggcgccgcc ttcgtcagcg
     1441 aaatgaaagc tcactccttc cggcacgtga acgcgggctt cgctccgata gaggctgaag
     1501 tcctcggcgt gcccggccgg caccgcatcc aggcgcaggc agcgcagcag ataatcgtag
     1561 gcgcgaatct cgcactccgg atcggacacc gggccgagct ccagcgtgac cgccggacac
     1621 cggccggcaa aaaggtgggt aatgacggta tccggttcct cgatcagcac ggccttgtcg
     1681 cagaagagat aggccatgcc caggttctcc accgacagat cggagagaac agagtaatgc
     1741 gggttgtggc cagtgttgtt gtgcagatcg acggcggcca gaaaccggcg ctcggccaac
     1801 cggccagcca cgccccgcgc catctcaccc tcaacaccac ctgccccccg ccagacgcga
     1861 ttgaaatcct gctgacccgg caacgcccgc atccccgccc gggcggcttg cacgttgccc
     1921 acgaaaatcg acatggaccg gggcagttcg gggtggtcgg caagaagccg acgcagaccg
     1981 ttccagccgc tcggctcgtt gccgtgcaac agcacggaca cgaacagtgc cggctcccgc
     2041 ccgccgggca gatgaatcag gctgggcaaa ccgagggccc cttccagctc ccgggcttca
     2101 cactccagca gacccggcgg cagcgagtgg aactcgttga aaggcaccga ctagagagac
     2161 caggtgtg
//
LOCUS       DBNHOX010000625         3017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1529166, whole genome shotgun sequence.
ACCESSION   DBNHOX010000625 DBNHOX010000000
VERSION     DBNHOX010000625.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1529166"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1293)
                     /locus_tag="AC16UZ_13725"
     CDS             complement(16..1293)
                     /locus_tag="AC16UZ_13725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HIR6247791.1"
                     /translation="MERGLFELHEEPERADRLVWSRRRFIAGGVVATMERTLSAAIPY
                     GRFLPAGMIPVAIAESQPGTLVAEKSGLRVLNDRPLNAETPAHLLDDEITPAELLFVR
                     NNGVPPAAADPETWQLSVTGEACLAPRTFSLAELKREFRQYTYRLQLECGGNGRAEFR
                     PRVPGNQWTTGAIGCPEWTGVRLKDVLERVGVRGDAAYVAYRSADTHLSGDGSKDVIS
                     RGVPLSKALEAESLLAFQMNGEPIPHIHGAPLRMVCGGWPGSVSGKWVTEVLLRDRVH
                     DGAKMLGSSYRVPCRPVAPGESVSDEGMCIIQSMPVKSLITHPRSGSSAVVGRPMEVR
                     GHAWAGDRSIAEVAVSVDFGATWQPAVLARPANRLAWQRWRATVKLPVPGYYEIWARA
                     IDSAGVSQPMLVPGWNPKGYLNNAAHRIALRAG"
     gene            complement(1331..2515)
                     /locus_tag="AC16UZ_13730"
     CDS             complement(1331..2515)
                     /locus_tag="AC16UZ_13730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6247792.1"
                     /translation="MREVVIVDAARSAVGRKKGTLGFVHPTDVLGQVLMKLVERNGLE
                     PSQVDQVIGGCINKLGAQGMNVTRTAWLAHGGAEGTPCITIDSQCGSSQEAATLATGI
                     VAAGMADVVIACGVENMTRLPIGSDSMGLDSGLGKPVARSYFSRYEFTSQFEGAERMA
                     EKYQITRDDTDAFGLESQVRAARAQAEGRFESQIIPLEVPVFEEDGTRTDEAVVFDTD
                     EIPRDTSLEALAALKPVAREDGVHTAGTSSQIADGGAAVLIMSAEKAAELGLQPLARV
                     VETALVGCDPVLMLEGPIPATERLLERSGLTINQIDVFEVNEAFASVVLSWAKTVGAD
                     MAKVNPNGGAIALGHPLGATGCFLITKAVHELKRSGGDYALVSMCCGGGLGTGTLLQR
                     VG"
     gene            complement(2655..>3017)
                     /locus_tag="AC16UZ_13735"
     CDS             complement(2655..>3017)
                     /locus_tag="AC16UZ_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247793.1"
                     /translation="VCAWVGAAEDLSGLEPEAQDWFQREELPDTLHDLLAEVGRVYVP
                     VMLANAQAVESGDELVRAQVDGKRWEQLPFPYQAKCLQWIRQAFARLDGEDRDYVKGV
                     LDGTGCEALVSKPPLELV"
BASE COUNT          534 a         1056 c          885 g          542 t
ORIGIN      
        1 aaaaggcctt cgccatcaac cggccctgag ggcgattcgg tgggcggcgt tattgaggta
       61 ccccttggga ttccatcctg ggacgagcat gggctgggac acgccggccg aatcgatcgc
      121 gcgggcccag atctcgtagt agccgggcac ggggagcttt accgtggccc gccatcgctg
      181 ccacgcgagc cggtttgccg ggcgcgccaa taccgcaggc tgccaggtcg caccgaaatc
      241 aacgctcacc gcaacctccg ctattgaccg atcgccggcc caggcatgcc cgcgcacctc
      301 catcggccga cccaccacag cgctgctgcc gctgcggggg tgggtaatca gcgatttcac
      361 cggcatggac tggatgatgc acatgccctc gtcgctgaca ctctcgccgg gcgccacagg
      421 ccgacaggga acgcgatacg agctgccgag catcttcgct ccgtcgtgca cccgatcgcg
      481 caagagcacc tcggtgaccc atttcccgga taccgatccc ggccaacccc cgcagaccat
      541 gcgtaacggc gcgccgtgaa tatgcgggat gggttcgccg ttcatctgaa acgcaagcaa
      601 cgactccgct tccagcgcct tgctcaacgg gaccccgcgg gaaatcacgt ccttggaccc
      661 atcgccggac aggtgggtgt cggcgctccg ataagccaca tacgcagcgt caccccgcac
      721 gccgacccgc tcgagcacgt ccttgagccg cacgcccgtc cactccgggc aaccgatcgc
      781 tcccgtcgtc cattgattgc cggggacgcg gggtcgaaac tccgctctgc cgttgccccc
      841 gcattcgagc tgaaggcggt acgtgtattg cctgaattcc cgctttagtt ccgccaggga
      901 gaaagtgcgt ggtgcaaggc atgcctctcc ggtcacggag agctgccagg tttcaggatc
      961 cgcggcggcg gggggcacgc cgttgttgcg cacgaacaga agctcggccg gcgtaatctc
     1021 gtcgtccagc aggtgcgcgg gtgtctcggc atttagcggt cgatcgttga gaacgcgcaa
     1081 tccggacttc tccgcgacca acgtgccggg ttgggattcc gcaatcgcca cgggaatcat
     1141 gccggcaggc aggaagcggc catagggaat ggccgccgac agcgtcctct ccattgtcgc
     1201 gaccacgccg ccggcaatga accgacgcct ggaccagacc agccggtcgg cgcgttccgg
     1261 ttcctcgtgc agctcgaaga gtccgcgctc catcgggtct caccgctcgt tcagcgccct
     1321 ggaacgctgc tcaaccgacc cgttgcagga gcgtccccgt gccgagcccg ccgccacagc
     1381 acatgctcac gagcgcgtaa tcgccacccg accgcttgag ctcgtgcacg gccttggtga
     1441 tgaggaagca gccggttgca cccagcggat gcccaagggc gatggcgcct ccgttgggat
     1501 tgaccttggc catatcggct cccacggtct tggcccagga cagcacaacc gacgcgaacg
     1561 cttcgttgac ctcgaacacg tcgatctggt tgatggtcag accggacctt tcgagcagac
     1621 gttcggttgc aggaatcggc ccttccagca tcagcacggg atcacagcca accagagccg
     1681 tctccaccac ccgggccagg ggttggagac cgagctcggc ggccttctca gcgctcatga
     1741 tgagcacggc ggctccacca tcggcaatct gcgacgaggt gccggccgtg tgcacgccgt
     1801 cctctctggc aacgggcttg agtgccgcca aagcctccag gctggtgtcc cgcggaatct
     1861 cgtccgtgtc gaacacgacc gcctcgtcgg tacgcgtgcc gtcctcttcg aacaccggca
     1921 cctccagcgg gatgatctgt gattcgaatc gaccttctgc ctgtgcgcga gcggcgcgca
     1981 cctgagattc caggccaaac gcatcggtat cgtcacgggt gatctggtat ttctccgcca
     2041 tgcgttccgc accttcgaac tggctggtga actcgtagcg ggagaaatag cttcgcgcca
     2101 ccggcttgcc gagccccgag tcgagcccca tggagtccga tccgatgggc agccgcgtca
     2161 tgttctctac cccgcaggca atgacgacgt cggccattcc ggcagcaacg atgcccgtgg
     2221 caagcgtcgc ggcctcctgg gacgagccgc attgggaatc gatggttatg cacggcgtgc
     2281 cctccgcgcc gccatgggca agccaggccg tgcgggtcac gttcattccc tgagcgccca
     2341 gcttgttgat acaaccgccg atgacctgat caacctggct cggctcgaga ccatttcgtt
     2401 ccacgagctt catgaggacc tgtcctagga cgtcggtcgg gtgtacgaac cccaacgtgc
     2461 ccttcttgcg cccgacggcc gatctcgccg catcgacaat taccacttct ctcatggtct
     2521 ggtttccttt ctatctcgtt aacaacgaat ctagttatcc cttcacgtgg tgggtatctc
     2581 cttcccggcc cacaccctgc ttcgaccgca tcacccccat ggacaacggt ccggggccgg
     2641 ctgggctgac gacgtcacac cagctccagc ggtggcttgg aaacgagcgc ctcgcacccg
     2701 gtaccgtcga ggacgccttt cacgtagtcg cgatcctccc catccagacg ggcaaatgcc
     2761 tggcggatcc actgcagaca tttcgcctgg tacggaaacg gcagctgttc ccagcgcttt
     2821 ccgtcaacct gggccctgac cagctcatcg cccgattcga ccgcctgtgc gttcgcgagc
     2881 atgacgggaa catagacccg gcccacctcc gccagaagat cgtgcagcgt atctggaagt
     2941 tcctcccttt ggaaccaatc ctgagcctcc ggctcgagtc ccgacagatc ctccgccgca
     3001 ccgacccatg cacacac
//
LOCUS       DBNHOX010000626         2443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_372338, whole genome shotgun sequence.
ACCESSION   DBNHOX010000626 DBNHOX010000000
VERSION     DBNHOX010000626.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_372338"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="AC16UZ_13740"
     CDS             complement(<1..489)
                     /locus_tag="AC16UZ_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247794.1"
                     /translation="MAHAKPLVVDMDSHVLEPPDLWQRYLEPKYRHRAIRIEQGADGI
                     ETLFVDDKALLSGRLAALGGAEHDAVRTFTDPDLTYLDGCPKASYDTQARVQLLDEWG
                     VDMGVVFPTIGILWDKEDDPELAMAYARAYNNWQWDFAKPALDRILPIAHVPLYDVDL
                     AYE"
     gene            880..>2443
                     /locus_tag="AC16UZ_13745"
     CDS             880..>2443
                     /locus_tag="AC16UZ_13745"
                     /inference="COORDINATES: protein motif:HMM:NF016292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HIR6247795.1"
                     /translation="MVNSTSVLGRLSFGLIAIAAAAAILLFSDLEARKDRTVSDADDA
                     AKVWRVALVQLAPVQPVDEGVRGILAGLAARGYVDGDRLEVIQYNAQGDLAVANAIAA
                     QVTSAGLDMIITASTPSLQTVANANRSRANPTVHIFGISSDPYGAGVGISREDHLDHP
                     PYMAGLGSLPPVRELFDLVRTINPAARRVGLVWNPTEANSEAATHLARAAAAEFGMEL
                     VEGNAESSTAASEVAQSVISRGIDVLWVSPDSTVYTAIDALIEAARRGGVPVVTSLPG
                     SVGRGSLLDLGARYEDIGYVQGLLAADVLEGRDPASVPVENWIPVDLHLNLTVLDGIS
                     RSWRIGPDLVASASVVIDDSGTRERGLPKPEPPEWLRWDRLAEGGQVPNVDLVMYSES
                     PVAEQSIEGVRLGLAQGGFDPDLRLNLRVHSAQGDMPTLVSIIDAIRPTSTELIMTLS
                     TPALQNALQRVRGIPIVFGMVSNPFIVGAGISDTDHVPTATGAYLNQPLESALDIVAE
                     LFPEARTLGTLFT"
BASE COUNT          404 a          781 c          815 g          443 t
ORIGIN      
        1 ctcgtaggca agatccacgt cgtacagggg aacatgagcg atcggcagga tgcgatccaa
       61 agctggtttg gcaaaatccc actgccagtt gttgtaggcg cgcgcgtacg ccattgccag
      121 ctcgggatcg tcttccttgt cccacagaat tcctatggtg gggaacacca cgcccatgtc
      181 cacgccccac tcgtcgagca gctgcacgcg cgcctgggtg tcgtaagaag ctttcggaca
      241 gccatcgagg taggtcagat ccgggtctgt gaaggtccga acggcgtcgt gctctgcgcc
      301 gccgagcgcc gcgagccgcc cggaaagcag agccttgtca tccacgaaga gcgtttcgat
      361 gccatcggct ccctgctcga tgcggatggc ccgatgccgg tatttgggct cgaggtatct
      421 ctgccacaga tccggcggtt ccagcacatg ggagtccata tccactacca acggctttgc
      481 atgggccaca tgtttctccc atcactggct gttcgcgaaa gtataacgcc cgtatctggc
      541 gaggttggcg gaattcgcct tgaatcggtc gaagggccga gggtgtcgag ctgatcgctg
      601 cgatccgaag cggggcttcg gtggggatcg gtggcggtga cgcggccgcc gcgctcgagg
      661 aggtacagcg tcgcggccca tccctcaccg tctgggagcc cgtcgcgcgt caggcccgga
      721 aagaaggcca tgacggcctc cgcggaagcc cggtgcgtga tgtactcgat gctgggctgg
      781 tagccgggcg ggcccttctt ctcgcaacca gagctttttt gcttttgttt tcccaacgta
      841 agatgtctta tacaggttgg ggggaagtgt agattctcga tggtgaactc cacgagcgtg
      901 ttgggcaggc tctctttcgg gttgatcgcg atcgcggcgg ccgccgcgat ccttctgttt
      961 tccgacctcg aggcccgaaa ggaccggacg gtttccgacg ctgacgatgc cgcaaaagtg
     1021 tggcgggtcg cgctggtgca gctcgccccc gtgcagccgg tggatgaagg ggtccggggg
     1081 atactggcgg ggctcgcggc gcggggttac gtggatggtg accgcctcga agtgatccaa
     1141 tacaacgccc agggcgacct ggccgttgcc aacgccatcg ccgcgcaggt caccagcgca
     1201 ggcctggata tgatcatcac ggcctcgacc ccctcgctgc agaccgtggc gaacgccaac
     1261 cggtcccggg cgaatccgac cgtgcacatc ttcggtatct cttccgaccc ctacggcgcc
     1321 ggggtcggta tcagtcgtga ggaccacttg gaccacccgc cctacatggc cggcctgggc
     1381 agcctgccac ccgtgcggga gctgttcgac ctggtgcgga ccatcaatcc tgccgcgcgc
     1441 cgggtgggcc tcgtgtggaa cccgacggag gcgaactccg aggcggccac ccacttggcc
     1501 cgcgccgccg ccgccgagtt cgggatggag ctcgtggagg ggaacgcgga gtcatcgacg
     1561 gcggccagcg aggtggcgca gtccgtgatc tcgcggggga tcgacgtcct ctgggtgagc
     1621 ccggacagca ccgtctacac tgccatcgat gcgctcatcg aggctgcccg tcgcggcggc
     1681 gttccggtcg tgacttccct gccgggttcg gtcggccgag gctccctcct ggacctgggt
     1741 gccaggtatg aggacatcgg ctatgtgcag ggtcttctcg ccgccgacgt cctcgaaggg
     1801 cgggatcctg cgagcgtccc ggtggagaac tggatcccgg tcgacctgca cctcaacctt
     1861 acggtgctgg acgggatctc ccgcagctgg cgcatcggac ccgacctcgt ggccagcgcg
     1921 agcgtcgtga tcgatgactc cggcacgcgg gagcgagggc tacctaagcc cgaaccgccc
     1981 gagtggctgc gctgggaccg gctcgccgaa ggagggcagg tgccgaacgt cgacctggtc
     2041 atgtactcgg agagcccggt tgcggagcaa tccattgaag gggtcaggct gggccttgct
     2101 caggggggct tcgaccccga tcttaggctc aacttgcgcg ttcactcggc ccagggggac
     2161 atgccgaccc tcgtcagcat catcgacgcc atcaggccca cgagcacgga gctgataatg
     2221 actctaagca cgccggcgct ccagaacgcg ctgcagcgag tccgcgggat cccgatcgtc
     2281 ttcgggatgg tgtcgaaccc cttcatcgtc ggagccggca taagcgatac ggatcacgtg
     2341 ccgacggcca cgggcgccta cctgaaccaa ccgctcgagt cggcgttgga catcgtcgcc
     2401 gagctcttcc ccgaagcccg gacgctggga acgctgttca cgc
//
LOCUS       DBNHOX010000627        16905 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1463800, whole genome shotgun sequence.
ACCESSION   DBNHOX010000627 DBNHOX010000000
VERSION     DBNHOX010000627.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 16905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16905
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1463800"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="AC16UZ_13750"
     CDS             <1..495
                     /locus_tag="AC16UZ_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015371271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR6247796.1"
                     /translation="TTGPEILKDFAGRRLDYWVTGWGTGGTLTGAGEVIKVARPDVKI
                     IAAEPEQASMLAGKEWSPHMIQGWTPDFIPAVLNPEVCDAIVPVNQDVAKDTALALAR
                     NEGIFVGVSAGGTLAAALDVAEDAAPGSVFVVMLPDTGERYLSTFMFEGINEGSDDDW
                     LESL"
     gene            565..1773
                     /locus_tag="AC16UZ_13755"
     CDS             565..1773
                     /locus_tag="AC16UZ_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6247797.1"
                     /translation="MAELSLESIFTPEIIADPYPLYRQLRETSPVLELPDANMVILTR
                     YADVQTVLRDRRLGHADESMLTRGDLEQARNNPAIANLRRTMLLKNPPDHTRLRGLVV
                     KAFDARRVEAMRTRIRAIANELVDAFVDAGSGDLVRLFTHPLPVIVICDLLGVPKADQ
                     AEFVQGTRISGRLIDPSPMTEEELAQANASTTESQRYFEQLCDERRQAPRDDLITALV
                     QSETEHGKLSKEELTSNISLLFAAGHETTVNLMGNGLIALYRNRDQLDRLRADPSLMP
                     NAVEEFLRYDSSVQLTGRDALEDAEIAGIALPRGRAVLALLAAANRDPEAFDRPEELD
                     IARKRIKPVSFGGGIHLCLGAQLARIEAHEALNVLLERLPNLELDDVATPDWKQTITL
                     RGVRTLPATW"
     gene            complement(1798..2226)
                     /locus_tag="AC16UZ_13760"
     CDS             complement(1798..2226)
                     /locus_tag="AC16UZ_13760"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6247798.1"
                     /translation="MIELDEEIKGRLDRAIEEHKTVTVAYVDVDGKPHISFYGSTHVH
                     SSNQLAIWVRNPQNDLIRTIPDRPNVAIIYGDIGGRVYYTFEGTASVTDDAEEQERVY
                     KEMHPVERQFDPDRGGVAVVVDLDRVTLLSAAAGKQVMER"
     gene            2424..2780
                     /locus_tag="AC16UZ_13765"
     CDS             2424..2780
                     /locus_tag="AC16UZ_13765"
                     /inference="COORDINATES: protein motif:HMM:NF023178.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HIR6247799.1"
                     /translation="MPEILFLLVAGHAICDFVLQSEAMATGKNHRLKEKMRERHGPSF
                     PSWPYWLTSHTITHGAAVFLITGNLLLGMIETVLHTVIDYTKCAGRISLLTDQILHGA
                     CKIGYVYYLWHAGALG"
     gene            complement(2801..2929)
                     /locus_tag="AC16UZ_13770"
     CDS             complement(2801..2929)
                     /locus_tag="AC16UZ_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247800.1"
                     /translation="MSGQDQGGFRANETIARLVAFVGPYILWGTLGLVVWGMLLTA"
     gene            complement(3455..4276)
                     /gene="cysQ"
                     /locus_tag="AC16UZ_13775"
     CDS             complement(3455..4276)
                     /gene="cysQ"
                     /locus_tag="AC16UZ_13775"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HIR6247801.1"
                     /translation="MDAELTNALIDLTRRAGDAILEIYQTDFDVEHKGDSSPLTQADL
                     ASHAVIAAGLAELIPDVPVLSEESSPPEFAVRQGWSRYWLVDPLDGTKEFVSRNGEFT
                     VNIALIEGHRAVFGVVGVPTQGYVYTGDLATATALRHGPEGAVPLRGRKRNDSDPLVV
                     VASRSHGGQRLEDYLDALARSIGEIERKPVGSSLKLCILAEGHADLYPRLGPTSEWDI
                     AAAHAVLEAAGGAVWAVGGGALSYNAKESFLNPEFFAAADGSYPWRKVLPAVPAA"
     gene            complement(4286..4843)
                     /gene="nudE"
                     /locus_tag="AC16UZ_13780"
     CDS             complement(4286..4843)
                     /gene="nudE"
                     /locus_tag="AC16UZ_13780"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008736.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HIR6247802.1"
                     /translation="MPVPEIKAVRTVASTRFFRIEEIDLEFATGERRTYERLPGVGTP
                     AVIVVPVNERGELVLIREYCAGFHEMQLTMVKGAANRDEALEDAANRELKEEIGMGAR
                     QVRFLKCLSVAPGHMGFTINVMLATDLYPEQLPADEPEPPELVPWPLARLDELIAGDE
                     FREARAIAALSVARPWLDGRLDNEE"
     gene            4924..5580
                     /gene="yrfG"
                     /locus_tag="AC16UZ_13785"
     CDS             4924..5580
                     /gene="yrfG"
                     /locus_tag="AC16UZ_13785"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF011564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HIR6247803.1"
                     /translation="MICWDSVDTVLLDMDGTLLDLNYDNTLWNQLVPMRYSEAKSIPV
                     EAARELLFARMLDTRGQLDFYCLDHWAEFTGLDIVGLHRELTDLIRYRPYAQEFLVWL
                     RARGKRSLLVTNAHRESLKVKDAHSDVTSKLDADVSCHDYGEPKESPAFWAELMARHP
                     YEPARTLLIDDNDAVLTAAREFGIGHLLTVAQPDSGRPPREGLGYPAFTDFREILPDE
                     "
     gene            5573..5995
                     /locus_tag="AC16UZ_13790"
     CDS             5573..5995
                     /locus_tag="AC16UZ_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR6247804.1"
                     /translation="MSEGQAARLDRWLWAARFFKTRAQAKAAIEGGKVHLHLNRDESG
                     ATVRPKVSREVSVGDVVTVRRGGAPETVVIRAIAHQRGSASVAATLYDETPASIEGRE
                     AQRAQRQLERLGLKVPSTKPGKRDRRALKKLKEDSAPS"
     gene            5992..6888
                     /gene="hslO"
                     /locus_tag="AC16UZ_13795"
     CDS             5992..6888
                     /gene="hslO"
                     /locus_tag="AC16UZ_13795"
                     /inference="COORDINATES: protein motif:HMM:NF013589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIR6247805.1"
                     /translation="MTADRLHRFSFQNAPIRGNWVRLDAVLAEVFARQSYPGRLQAVL
                     AEALSAVAMLADTVKFQGTISLQSRGRGPVTTLLAECRSRSVLRGIARWDRSAGIPED
                     APLGELLGDARLAISLVDSDPATPRPVSYQGLVTLDHESLAGNLEGYFAFSEQLPTRF
                     YFGRAGHTVTGLLLQRLPADDDATEIDLDQHESDWKQIQEAAGESSADLAALPPQALL
                     RRLFPSRPLTLQPGRPLSFGCTCSRDRSTASLNAVPAPELLDILHSDGEVTVTCEICG
                     TVYAYDPIDIHLLLQREAPQLH"
     gene            6982..8547
                     /locus_tag="AC16UZ_13800"
     CDS             6982..8547
                     /locus_tag="AC16UZ_13800"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HIR6247806.1"
                     /translation="MAVDIESTAKHTTHLNLAAAALAELAVLRGEGQFASNGAIVVRT
                     GHRTGRSPRDRFIVEEPSTADAIDWGTINQPIAQDVFDALWDRVQIHLEDRERFVSNL
                     HVGADPEHYLPIEVTTEYAWHALFGRALFIKPENYNPHNKSVWNIANAPSFVCDPERD
                     GTASDGTVMINFAQRKVLLAGMRYAGEMKKSMFSVLNFLLPEKDVLPMHCSANMGHDG
                     AVTLFFGLSSTGKTTLSADPDRLLIGDDEHGWGKGTVFNFEGGCYAKCIDLTRENEPL
                     IYDAIRFGAIVENVIIDQDTREPDYTDGSLTENTRACYPRSNIEAKVTDNRGGEPGQV
                     VFLTCDVSGVLPPVSLLSKEAAAYHFLSGYTAQVGSTEVGSTEAYKATFSTCFGAPFF
                     PRAAGVYADLLIKRIEEFGSKVFLVNTGWTGGGYGVGKRFAIPDTRAIIRAIQSGALD
                     DAETRRLPGFNLDIPTSVPGVDAGLLNPRDTWSDKAAYDESARSLIAKFAENFEKFSV
                     AQAIVDAGPTTDY"
     gene            8587..10158
                     /gene="gshA"
                     /locus_tag="AC16UZ_13805"
     CDS             8587..10158
                     /gene="gshA"
                     /locus_tag="AC16UZ_13805"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HIR6247807.1"
                     /translation="MAHAAAPIDPALLDALRDQPASLREIQRGIEKESLRVTPEGSLS
                     DRPHPAALGSALTHPNITTDFSEAQLELITEVHQSPEATLAQLEGVHRFVYQNLDGEL
                     LWSASMPCVLGTDEEIPVGRYGGSNIARAKTIYRLGLGNRYGRLMQTISGIHYNFSVP
                     QTFWKVLAESRGKEPTQEFVTEAYFGLIRNFRRFSWLLIYLIGSSPAICKSFVKNQPH
                     ELASFDEGSLYLPHATSLRMGRLGYQSDAQRSLHISYNGLEQYAASMHTALTRPYPPY
                     EAIGVKVDGEYRQLNASLLQIENEFYGTIRPKRRIVSGERPLNALRSRGVEYVEVRCL
                     DLNPFLHMGIDTVEIRFIDAFLLYCLLADSPEDSERESELMGKNQLAVVERGREPDLQ
                     LEQLRGTITRKAWAEDVLHACRPLAELLDEATGSNLYRHALSEQSSKNRQPALTPSAR
                     VLAVMQTQGIPFFRFAMNQSIAHKGYFDEHPMRGEQLAEHQATSQQSLARQQEIEAAD
                     SVDFDVFLKDYLALE"
     gene            10155..10796
                     /locus_tag="AC16UZ_13810"
     CDS             10155..10796
                     /locus_tag="AC16UZ_13810"
                     /EC_number="3.1.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF016239.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HIR6247808.1"
                     /translation="MNFLAHCLIAAQSTDPEEPDLVAGGFLGDFVKGPIGPELPPGLA
                     LGVRLHRRIDAFSNRHPAIQGSCNRFAGPLRRLAPVLVDIIADHCLAKHWEQFSNEPI
                     AAFTARTYDRIASHERWLPDHGHRFLSYMRDEDLLAGYARFEAMHRALCSVTRRLDRE
                     HLNADMLSATEANLPGLEADFLGYFPELVAHARQWLRDEGPPPGPQTPGTVQR"
     gene            10869..11846
                     /locus_tag="AC16UZ_13815"
     CDS             10869..11846
                     /locus_tag="AC16UZ_13815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HIR6247809.1"
                     /translation="MKAVLCKAFGPPEDLVMEEVANPEPGPGQILVDIHASALNFPDV
                     LMIAGKYQSLPPFPFSPGGEIAGTVAAVGDGATRFKVGDRVFGGTGHGGFAEQIALPE
                     MAMRPMPDEMSFVEASAISTTYGTSYYALKQRADLKPGETLLVTGAGGGVGLAAVELG
                     KAMGARVIAAASSAEKLAAARSAGADELIDYSDGELKEKVKALTDGKGADVIYEPVGG
                     DLFEQCMRCVNWYGRILVIGFAAGDIPRVPINLILLKSCQVVGVFYGAWSARCRDEAD
                     ANFKEILDLYRDGKVKPMIGRTYPLEEYAAALRCLSERQAIGKVVVTVR"
     gene            11843..12340
                     /locus_tag="AC16UZ_13820"
     CDS             11843..12340
                     /locus_tag="AC16UZ_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HIR6247810.1"
                     /translation="MNAWVFRDTSELWAVMLVASVGALLFAALIMEHVFGMAPCPLCL
                     MQRIWFFVAGAFAFASLLHNPRLGIYPLCSMVAAVIGGGFSIRQLYLQGLPPDQVPSC
                     GPDMSYMLEAFPLTDVLIAMTRGTGDCAEVAWSFLGISIPGWALVGFAALAVMAALQL
                     RAGTR"
     gene            12435..13625
                     /locus_tag="AC16UZ_13825"
     CDS             12435..13625
                     /locus_tag="AC16UZ_13825"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6247811.1"
                     /translation="MSRAQLINMARHNIGHAKAGTIEQAPDIVKVPVQNYFDPDRWQL
                     EMDRIFRRMPLVLAMTDELRNPGDYKAMEAVGMPVLLSRGEDGEVRAFVNMCRHRGSQ
                     VMLPGCGNAHRFTCPYHAWTYDQEGALVGVYAPKDFGDVDKEENGLVPLPVAERAGMI
                     WVIVNPQATLDIDTFLSGYDELLGHFHFEDWHLFEQRTIAGPNWKIAYDGYMDLYHLP
                     ILHKDTFGDQFPNQALYYAWGPHQRVSSPDPTLAGYEQLPESEWNEAHMLAGVWTIFP
                     HVSVAGFDGGGRSVMVSQLFPGKTPQESVTVQNYLMEKAPDAEQAEAAEQMFKLLEYV
                     VQEEDYATGLRQQKAIMTGAMSHVMFGRNEEGGQRFHRWLDTLLATDDEDLNALFAGG
                     ERYC"
     gene            13885..14637
                     /locus_tag="AC16UZ_13830"
     CDS             13885..14637
                     /locus_tag="AC16UZ_13830"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HIR6247812.1"
                     /translation="MNANVRRILISNGKGGCGKTTIAANLAVSYANRGAKVALVDQDP
                     QASSSYWADQRSRELAPVHLVRAHQRANMYQTQTFHNRIPADISRVVVDGSTNPQDRE
                     LEMLLKQCDVILVPLLPSAIDIRAGSQFIKELITHRSFRTLPKPVGVIANRVQPNSTM
                     HARLEHFLKCLDVPWVATFRDSPLYPEAFERGEGVVDLIDCRAARREAPAWRALTAWI
                     ERQPVSRGCTARELRNRPIRAPRHVDDETLSA"
     gene            14739..16619
                     /locus_tag="AC16UZ_13835"
     CDS             14739..16619
                     /locus_tag="AC16UZ_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003161181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR6247813.1"
                     /translation="MLQLIDISLKRGSNQLFEHLTCTIHPGHKVGMVGRNGAGKSTLF
                     DMILGRLQPDEGEVSRPSGWVASHMAQEVNVTDRPAIEHVIDGHRELRAVERRIAQAE
                     SRRDDLTLAELHTRFGDVGGHQARARAGTILHGLGFAADEIEQPFRAFSGGMRIRLNL
                     AQALMAPADLLLLDEPTNHLDLETTVWLESWLQRFAGTLLVIAHDRAFLDGVCDHIVH
                     IEHGRANSYRGNYSSFERQRAEALAQQQAAHARQQAEIKHMQHFIDRFRAKASKAKQA
                     QSRMRALERMQAVAPVHADSPYRFSFANPEQMSHPLLSLEDVSVGYEGRAVIEHVRQT
                     LLPGARIGILGANGAGKSTLLKCLVGELEPLAGTLVRGRHSAVGYFAQHQLERLDGQT
                     SALATLTNERPQAREQWCRDYLGRWGFPADMVERPIVTLSGGEKARLVLALIALAQPA
                     ILVLDEPTNHLDLEMREALALALQDYAGALLLVSHDRSLLRRAVDEFWLVEGGRLASF
                     AGDIDAYAESRLRRSEPPSSERAPETNRKALRQAAAAQREAAKPLRAKLRHLEKEMDQ
                     IGVELREVESRLADGDVYRSLPPEELDDLLSAAGRLRKRLDATEQAWLSATEALEALE
                     QS"
     gene            complement(16658..>16905)
                     /locus_tag="AC16UZ_13840"
     CDS             complement(16658..>16905)
                     /locus_tag="AC16UZ_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247814.1"
                     /translation="MSDGTIDAGVLRNGKKREVSIDCSQFSAGDHMAITIDPSIIRIR
                     RGIDGDDGVDVRIVVDEEVVVDEKGSKKAAADDSAGG"
BASE COUNT         3071 a         5663 c         5374 g         2797 t
ORIGIN      
        1 accaccggcc ccgagatcct gaaggacttc gccgggcgcc ggctggacta ctgggtgacc
       61 ggctggggca ccggcggcac gctgacgggc gccggggaag tgatcaaggt cgcccgcccc
      121 gatgtgaaga tcatcgccgc cgagccagag caggcatcca tgctggcagg caaggagtgg
      181 agcccgcaca tgattcaggg atggacgccc gacttcatcc ccgcggtgct caatcccgag
      241 gtgtgcgatg cgatcgttcc cgtcaatcag gacgtggcga aagacaccgc gctggcgctg
      301 gcgcgcaacg agggcatctt cgtcggcgtt tccgccgggg ggacgctcgc cgcggccctg
      361 gacgtcgccg aggacgcggc gcccggcagc gtgttcgtcg tgatgctgcc ggacaccgga
      421 gaaaggtatc tttccacctt catgttcgag ggcatcaacg aaggttcgga cgacgactgg
      481 ctcgagtccc tgtgatcggc cgccgagtca tgccgccgag ctgccctgac aaccggcagg
      541 tcggcgggcg aacggagagt aacaatggct gaattgagcc tcgaatcgat cttcacgccc
      601 gagatcatcg cggaccccta cccgctatac cggcagcttc gcgagacgag cccggttctc
      661 gagcttcccg acgccaacat ggtgattctc acccgctacg ccgacgtgca gacggtcctg
      721 cgggatcgcc gcctgggcca cgccgacgag tccatgctga cccggggcga tctcgagcag
      781 gcgcgaaaca acccggccat cgccaattta cggcgcacca tgctgctcaa gaacccgccc
      841 gaccacaccc gcctgcgggg tctggttgtc aaggctttcg acgcgaggcg ggtggaggcc
      901 atgcgcacgc gcattcgagc catagccaac gagctcgtgg acgcgttcgt cgatgccggc
      961 agcggtgacc tggtccgcct gttcacccat cctcttccgg tcatcgtcat atgcgacctg
     1021 ctcggcgtgc ccaaggcaga ccaggccgag ttcgtgcagg gcacgcgcat ttccggacgg
     1081 ctcatcgacc cgagccccat gacggaggaa gagctcgctc aggcaaacgc gagcaccacg
     1141 gagagccagc gttacttcga gcagctgtgc gacgagcgcc gccaggcgcc cagggacgac
     1201 ctgatcaccg ccctcgtgca gagcgagacc gagcacggca agctcagcaa ggaggagctc
     1261 acgtcgaaca tctctttgct gttcgcagcc ggccacgaga ccaccgtcaa cctgatgggc
     1321 aacgggctca tcgccctcta ccggaaccgg gaccagctcg accggctgcg cgcagacccc
     1381 tcgctcatgc ccaacgccgt cgaggagttc ctgcgttacg acagctccgt gcagctgacg
     1441 ggccgggacg cgctggaaga cgccgagatt gccggtattg ccctgccgcg cgggcgcgcc
     1501 gtcctggcgc tgctggcagc cgccaaccgc gaccccgagg cgttcgaccg cccggaggag
     1561 ctggacatag cccggaagcg catcaagccg gtgtcgttcg gcggaggcat ccacctgtgt
     1621 ctgggggcgc agctggcgcg aatcgaggcc cacgaggccc tgaacgtgtt gctcgaaaga
     1681 ctgccgaatc tagagctgga cgacgtggcc acgccggact ggaagcagac gatcaccctg
     1741 cgcggcgtgc gcacgctgcc ggcaacctgg tagtcggcgg cggagagcgt ccccccctta
     1801 gcgctccatg acctgtttgc cggcggccgc gctcagcagg gtgacccggt cgagatcgac
     1861 gaccacggca acgccacccc ggtccgggtc gaactgccgc tccacggggt gcatctcctt
     1921 atacacccgc tcctgctctt cagcgtcgtc cgtcaccgag gcggtaccct cgaaggtgta
     1981 gtacacgcga ccgccgatgt caccgtagat gatggcgacg ttgggccggt cgggaatcgt
     2041 tctgatcaga tcgttctgcg ggttgcgcac ccagatcgcc agctggttgc tgctgtgaac
     2101 gtgagtgctt ccatagaagg agatgtgggg cttgccgtcc acgtccacgt aggccaccgt
     2161 gacggtcttg tgttcctcga tggcccggtc cagccggccc ttgatttcct cgtccagctc
     2221 gatcatcgcg tctcctcctg gggctttggc tgcccgtctt tggtctatac tcgcttgcca
     2281 cgagggcgac ccctgcgtcg ccgaagcgcc gtcgccgccc gggcagaccg ggggctggag
     2341 cgcttcgcga ggggggcgaa acttaccata ctttcgatgc ggattgccgg ccgcggcccg
     2401 gtcgtccacg gattggacaa gcaatgcctg agatactgtt tcttctcgtt gcaggacacg
     2461 ccatctgcga tttcgtcctg cagtccgaag ccatggcgac cggcaagaac caccgcctca
     2521 aggagaagat gcgggagcgg cacggcccct ccttcccctc ctggccttac tggctgacct
     2581 cacacacgat cactcacggg gccgccgtat tcctgatcac gggaaatctg ctgctgggga
     2641 tgatcgaaac cgtgctccac acggtgatcg actacaccaa gtgcgccggt cggatcagcc
     2701 tgctgaccga ccagatcctg cacggcgcct gcaagatcgg ctacgtgtac tacctttggc
     2761 atgcgggcgc gctgggctga tccggcgctg gccgccctca tcacgcggtc agcagcatcc
     2821 cccagaccac aaggcccagc gtgccccaga gaatgtacgg gcctacgaaa gcgacgagcc
     2881 gggcaatggt ctcgttcgcc cgaaatcccc cctggtcctg tccgctcatc aacttctccc
     2941 agctacggac gcctgcggag cccccaattg ctccgcgtct cggggcgatg tccggctggc
     3001 tgaagagagt ggcgccggcg gaacgcccgt cttctctagg ttttatgccc ttcctaacta
     3061 tttggcaatt aaatttacgt gagaggatgt gcggcagatg taacgtggtg ctgctttgcg
     3121 gaatcggggt tcctgggaaa aatcggggaa aagtgaaaat aatatggatt cgacaacttc
     3181 aactgattga tttcaaaggc tattgccttc gactagccca tgaggccggc cagcaggctt
     3241 tttttccgga atgatttcgg tatattggtc cacctcacta gccggtgctg tatgtaaata
     3301 cagtgtaatt ctcctcggca gggcgaacac acgaacataa gcccggctag tgaggccaac
     3361 ctcccgacct ttcaagctct cccgaccact ctacctgctg gactgactga cgctcttctg
     3421 gagcccgtct acggccttgg gcgctggtgc ccgctcaggc agcgggcacg gccggcagaa
     3481 cctttcgcca ggggtaggag ccgtcggcgg cggcgaaaaa ttccggattg agaaaggatt
     3541 ccttcgcgtt gtaggagagc gctccgccac ccaccgccca gacggcacca ccggcagctt
     3601 ccagcaccgc gtgggctgcg gcgatgtccc attcagaggt aggtcccagg cgaggataga
     3661 gatccgcatg cccctcggcg agtatgcaga gcttcaacga gctgcccacg ggcttgcgtt
     3721 cgatctcgcc gatgctgcgg gcgagcgcgt ccaggtagtc ctccagccgc tggccaccat
     3781 ggctccggct ggccaccacc accagaggat cggagtcgtt gcgcttgcgt ccgcgcagcg
     3841 gcacggcgcc ctcggggccg tggcgcagcg ccgtcgcggt ggcaaggtcc ccggtataca
     3901 catagccctg ggtcggcacc ccgacgacgc cgaaaaccgc gcggtgaccc tcgatgagcg
     3961 cgatgttgac cgtgaactcg ccgttgcggc tcacgaactc cttggtgccg tcgagaggat
     4021 ccaccagcca gtagcgcgac cagccctgcc tcacggcaaa ctccggcggg ctggactcct
     4081 ccgagagcac gggcacgtca ggaatcagct cggcgagccc cgcggcaatg accgcatggg
     4141 atgcaagatc ggcctgtgtg agcggggacg aatcgccctt gtgctccacg tcgaagtccg
     4201 tctggtagat ctccaggatc gcgtccccgg cccgtcgtgt caggtcgatc agcgcgttgg
     4261 tcagctctgc gtccatctcg tctggctact cctcgttgtc cagtcgccca tcaagccaag
     4321 gccgcgcaac gcttagagcg gctatggcgc gggcctcgcg aaactcgtcg ccggcaatga
     4381 gctcgtcgag ccgagccagc ggccacggaa ccagctccgg cggctccggc tcgtcggcag
     4441 gcaactgctc gggatagaga tccgtggcca gcatcacgtt gatggtgaat cccatgtggc
     4501 ccggcgccac gctgaggcat ttgagaaacc gcacctgccg ggcgcccatg ccgatctcct
     4561 ccttgagctc gcgattggcc gcgtcctcca gagcctcgtc ccggttggcg gcgcccttca
     4621 ccatggtcag ctgcatctcg tgaaagccgg cgcaatattc ccggatcagc accagctcgc
     4681 cacgctcgtt caccggaacg acgatgacgg ccggcgtgcc cacacccggc aggcgttcgt
     4741 aggtgcgccg ctcaccggtg gcaaactcca ggtcgatctc ctcgatgcgg aagaaccggg
     4801 tggatgcgac ggtgcgaacg gctttgattt ccgggacggg cacggcgaac tctcctgctg
     4861 ctcggcacca agtccgagtg cgaaaccgct attatacggg ccccgaacgg acagtgacgg
     4921 caaatgatct gctgggactc cgtcgacacc gtcctgctgg acatggacgg cactttgctc
     4981 gacctgaatt acgacaacac cctgtggaac cagctggtgc cgatgcgcta cagcgaggcg
     5041 aagtccattc ctgtcgaggc cgcccgcgag ctcctgttcg cacgcatgct cgacacccgc
     5101 gggcagctcg acttctactg cctggaccac tgggcggaat tcacgggcct ggacatcgtg
     5161 ggcctgcatc gcgagcttac cgacctgatt cgctaccgcc cttatgcgca ggagttcctg
     5221 gtgtggctgc gggctcgcgg aaagcgctcc ctgctggtca ccaacgccca ccgtgaaagc
     5281 ctgaaggtga aggacgccca ctccgacgtg acgagcaagc tggacgcaga cgtgtcgtgc
     5341 cacgactacg gcgagccgaa ggagagcccc gccttctggg ccgagctgat ggcgaggcac
     5401 ccctacgaac cggcacgcac gctgctcata gacgacaacg acgccgtgct gacggcggcc
     5461 agagagttcg gcatcgggca cctgctcacc gtggcccagc cggattccgg gcgacccccc
     5521 agggaaggcc tgggctatcc ggcttttacc gacttccgcg agatcctgcc cgatgagtga
     5581 gggccaagcg gctcggctgg acagatggct gtgggcggcg cggttcttca aaacccgcgc
     5641 ccaggccaag gcggccatcg agggcggcaa ggttcatctg cacctcaacc gcgacgagag
     5701 cggggcgacg gtccggccga aggtcagcag ggaagtgagc gtcggagacg tggtcacggt
     5761 gaggcgaggg ggagcgcccg agaccgtggt gatccgggcg atcgcccacc agcggggcag
     5821 cgcatccgtc gccgcaaccc tctacgacga aacgccggca agcatcgagg ggcgagaagc
     5881 ccagcgcgcg caacgccagc tggaacggct cgggctcaag gtgccgagca ccaagccggg
     5941 caaacgcgat cgtcgagcgc tcaagaagct caaggaagat tcggcgccgt catgaccgcg
     6001 gaccgcctgc atcgtttcag ctttcagaac gcgccgattc gaggcaactg ggtgcgtctg
     6061 gacgccgtgc tggccgaggt gttcgcacgt cagtcctatc cgggccggtt gcaggcggtt
     6121 ctggccgaag ccctgagcgc ggtcgccatg ctcgccgaca ccgtcaagtt ccaggggacc
     6181 atcagcctgc agtcccgagg gcggggacca gttacaaccc tgctggcaga gtgccgctcg
     6241 cgaagcgtgc tgcgcgggat cgctcgatgg gaccgatcgg ccggcattcc ggaagacgcg
     6301 cccctgggtg agctcctcgg tgacgctcgg ctggccatct ctctcgtgga ctcggatccg
     6361 gccacacccc gtccggtgag ctatcagggt ctggtcaccc tggatcacga aagcctggcc
     6421 ggcaacctgg aaggctactt tgccttcagc gagcagctgc ccacacggtt ctacttcggc
     6481 agggcgggac acaccgtcac cgggctgctg ctgcagcggc tgcccgccga cgacgacgcc
     6541 acggagatcg atctcgacca gcacgaatcg gattggaaac agatccagga agccgcaggc
     6601 gaatcctccg ccgacctggc cgcgttgccc ccgcaagcgc tgttgaggcg tttgtttccc
     6661 agccggccgc tgacgctgca accgggacgg ccgctgtctt tcggctgcac ctgcagccgc
     6721 gatcgcagca ccgcctcact gaacgcggtg ccggccccgg agctgctgga cattctgcac
     6781 agcgacggcg aggtcacggt gacctgcgag atctgcggca ccgtctacgc ctacgacccc
     6841 atcgacattc acctgctgct gcaaagggaa gccccgcagc ttcactgagc gagcttccct
     6901 tacaatccct ttcattccga ataggccaaa tctcaccgct ctggcataat cgatcgattg
     6961 tcaggggaag acgctgaaag catggcagtc gacatagagt caacggcaaa gcacaccact
     7021 catctcaacc ttgccgccgc cgcgcttgcc gaactggccg tattgagggg ggagggtcaa
     7081 ttcgccagca acggcgccat cgtcgttcga accggtcatc gcaccgggcg ctcgccgagg
     7141 gatcggttca tcgtggagga gccgtcgacg gccgacgcca tcgactgggg aaccataaac
     7201 cagcccatcg cgcaggacgt attcgacgcg ctctgggacc gggtgcagat tcacctggag
     7261 gaccgcgaac ggttcgtttc caacctccac gtcggggcgg acccggaaca ctatctgccc
     7321 atcgaggtga ctacggagta cgcgtggcac gcgctgttcg ggcgggcact gttcatcaag
     7381 ccggagaact acaaccccca caacaagtcc gtgtggaaca tcgccaacgc accgagtttc
     7441 gtctgtgacc ccgagcgcga cgggacggcc agcgacggga cggtgatgat caactttgct
     7501 cagcgcaagg tgctgctcgc cgggatgcgc tacgcgggcg aaatgaaaaa atccatgttc
     7561 tcggtgctca acttcctcct cccggagaag gacgtcctgc ccatgcactg ctcggcaaac
     7621 atgggccacg acggtgcggt gacgctgttc ttcggcctgt ccagcaccgg caagacgacc
     7681 ctgtcggcgg atcccgaccg gctgctcatc ggcgacgacg agcacgggtg gggcaaagga
     7741 accgtcttca acttcgaggg cggctgctac gccaagtgca tcgacctgac ccgggagaat
     7801 gagccgctca tctacgatgc gatccggttc ggcgccatcg tcgagaacgt catcatcgac
     7861 caggacaccc gcgaacccga ctacacggac ggatcgctga ccgagaacac cagggcctgt
     7921 tatccgcgca gcaacatcga agccaaggtg accgacaacc gtggcggcga acccggccag
     7981 gtggtatttc tcacctgtga cgtgagcggc gtgctgccgc cggtttccct gctctccaag
     8041 gaagctgccg cctaccactt cctgtccggc tacacggctc aggtggggtc cacggaagtg
     8101 ggctctacgg aagcctacaa ggcaaccttc tccacctgct tcggggcgcc cttcttcccg
     8161 cgtgcggccg gtgtgtacgc cgatctgctg atcaagcgga tcgaggagtt cggctccaag
     8221 gtgttcctgg tcaacacggg ctggaccggc ggcggctacg gcgttggcaa acgcttcgcc
     8281 atcccggaca cgcgggccat catccgggcc attcagtccg gcgccctgga cgacgccgag
     8341 acccggcgcc tgccaggttt caacctggac attccgacat cggtgccggg cgtggacgca
     8401 ggtcttctca acccgagaga tacgtggtcc gacaaggccg cttacgacga atccgcgcgc
     8461 agcctgatcg caaaattcgc agagaacttc gaaaagttca gcgtcgctca ggccatcgtt
     8521 gatgctggtc cgaccacgga ttattgaggt agcctgaagg ccgccccgtt gggctaagag
     8581 gcggatatgg cccacgcagc cgcacccatc gacccggcgc tcctggacgc attgcgcgac
     8641 cagccggctt ccctgcgaga gatccagcgg ggcatcgaga aggaaagcct gcgggttacg
     8701 ccggaaggct cgctctcaga ccgaccgcat ccggccgccc tgggctccgc gctcacccat
     8761 ccgaacatca ccacggactt cagcgaggct cagctggagc tgatcaccga agtgcatcag
     8821 tctcctgagg cgacgctcgc ccagctggag ggcgttcacc ggttcgtcta ccagaacctg
     8881 gacggcgagc tgctctggtc cgccagcatg ccgtgtgtgc tcggcacgga cgaggagatt
     8941 cccgtaggtc gctacggggg ctcgaacatc gccagggcca agacgattta ccggctgggg
     9001 ctcggtaatc gctacgggcg gctgatgcag accatctcag gcatccacta caacttttcg
     9061 gtcccgcaga cgttctggaa ggtgctggcg gaaagcaggg ggaaagagcc gacgcaggaa
     9121 ttcgtaacgg aagcgtattt cggcctcatc cgcaatttcc ggcgcttttc gtggctgctc
     9181 atctacctga tcgggtcgtc gcctgcgatc tgcaaatcgt tcgtgaaaaa tcagccccat
     9241 gagctggcgt ccttcgacga aggctcgctc taccttccac acgccacgtc gctgcgaatg
     9301 gggcgcctgg gctaccagag cgacgctcag cgcagcctcc acatttccta caacggtctc
     9361 gagcagtacg cggcatccat gcacacggcg cttacccgac cttacccccc gtacgaggcc
     9421 atcggcgtca aagtcgacgg cgagtaccga cagctgaacg cctcgctgct gcagatcgag
     9481 aacgagttct acggaaccat ccggccgaaa cgccgcatcg tgagcggcga aaggcccctg
     9541 aacgccctga gatcccgcgg ggtcgagtac gtcgaggtca gatgcctgga cctgaacccg
     9601 tttctccaca tgggcataga caccgtcgag atccgcttca tcgacgcgtt tctgctttac
     9661 tgcctgctgg cggacagccc tgaggacagc gagcgggaat cggagttgat ggggaaaaac
     9721 cagctcgccg tggtcgagcg gggccgcgag ccggaccttc aattggagca gctgcgcggc
     9781 accatcacac gaaaggcatg ggcggaagac gtcctgcacg cctgccgtcc gctcgccgag
     9841 cttctggacg aggccaccgg gagcaacctg taccgccacg cgctcagcga gcagtccagc
     9901 aagaaccggc agccggcgct gacgccgtcg gcgcgggtgc tggccgtcat gcagacccag
     9961 ggcataccgt ttttccgctt cgccatgaac cagtccattg cccacaaggg ttacttcgac
    10021 gagcacccga tgcgaggtga gcagctggcc gagcaccagg ccacctcgca acagtccctg
    10081 gcgcggcagc aggagatcga ggccgccgac agcgtggatt tcgacgtctt cctcaaggac
    10141 tacctggctc tcgagtgaac tttctcgccc actgcctgat cgctgcgcaa tccaccgacc
    10201 ccgaggaacc ggacctcgtc gccggcggct ttctgggcga cttcgtcaag ggccccattg
    10261 gccccgaact gcccccgggc ctggctctcg gcgtacggct gcatcggcgc atcgacgcct
    10321 tcagcaaccg gcacccggcc atccagggga gctgcaatcg atttgcgggc cccctgcgcc
    10381 ggctggcgcc cgtgctcgtc gacatcatcg ccgaccactg cctggccaag cactgggagc
    10441 agttcagcaa cgagcccatc gcagccttca ccgcaaggac ctacgatcgc atcgcgtccc
    10501 atgagcgctg gctccccgac cacggccacc gcttcctcag ctacatgcgc gacgaagacc
    10561 tgctggcggg gtacgcccgg ttcgaagcca tgcaccgggc tctgtgctcg gtgacacgtc
    10621 gcctggatcg cgagcacctc aacgccgaca tgctcagcgc taccgaagca aacctgcccg
    10681 ggctggaggc cgatttcctg gggtacttcc cggaactggt ggcccatgca cggcagtggt
    10741 tgcgcgacga gggcccgccc cccggacccc aaacgccggg cacggtgcag cgctgaaacc
    10801 ggtactgcca ggttgacgct gccgaaccga ccgttactct tgcgttcttt gattttcagg
    10861 aagcatccat gaaagcggtt ctgtgcaagg cattcggccc accggaagac ctggtgatgg
    10921 aggaggtggc aaatccggag cctgggcccg gccagatcct cgtcgacatt catgcctccg
    10981 ccctgaactt cccggacgtg ctgatgatcg ccggcaagta ccagagtctc ccaccgttcc
    11041 ctttctctcc gggcggcgag atcgcgggaa ccgttgccgc ggtcggcgac ggcgccacgc
    11101 gtttcaaggt cggcgaccgg gtctttggag ggaccggcca cggcggcttc gccgagcaga
    11161 tcgccctgcc cgagatggcc atgcggccca tgccggacga gatgagcttc gtcgaggcct
    11221 ccgccatcag cacgacctac ggcacctcct actacgcttt gaagcagcgg gccgatctca
    11281 agccgggcga gaccctgctg gtcacgggag cgggcggcgg cgtggggctg gcggccgtgg
    11341 agctcggcaa ggccatgggc gcacgggtaa tcgccgcggc cagctctgcc gagaagctcg
    11401 ccgcggccag gagcgccggg gccgacgagc tcatcgacta cagcgatggg gagctcaagg
    11461 agaaggtcaa agcactcacc gacggcaagg gggcggacgt catctacgag cccgtcggcg
    11521 gcgatctgtt cgagcagtgc atgcgctgcg tgaactggta tgggcggatt ctggtcatcg
    11581 gcttcgcggc cggtgacatc ccccgcgtac ccatcaacct cattctgctc aagagctgtc
    11641 aggtggttgg ggtcttctac ggcgcctgga gcgcgaggtg ccgcgacgag gccgacgcca
    11701 acttcaagga gatcctggac ctgtaccggg acggcaaggt caagccgatg atcggccgta
    11761 cctacccgct ggaggagtat gcggccgcgc tgcgctgcct gagcgagcgg caggccatcg
    11821 gaaaagtcgt ggtcaccgtt cgatgaacgc ctgggtcttc agagatacca gcgagctgtg
    11881 ggcggtaatg ctcgtcgcca gcgtcggcgc gctcctgttt gcggcgctga tcatggagca
    11941 cgtcttcggc atggcgccat gcccactctg cctgatgcag cgaatctggt tcttcgttgc
    12001 cggcgccttc gcgttcgcca gcctgctgca caacccgcgg ctggggatct acccgctgtg
    12061 ctccatggtc gcggcggtga tcggcggcgg gttctcgatc cggcagctgt acctccaggg
    12121 cctcccgccg gaccaggtgc cgtcctgcgg cccggacatg agctacatgc tcgaggcctt
    12181 ccccctcacc gacgtgctga tcgccatgac ccggggaacg ggcgattgcg ccgaggtcgc
    12241 ctggagtttc ctgggaattt ccattcccgg ctgggccctg gtggggtttg ccgctctggc
    12301 ggtgatggcg gcgctgcagc tgcgcgcagg aacacgctga ccggtcccag ggagccacct
    12361 gccgctgagc aggcggcgct tgttgattgc ccgaggtgag ctatgctgtg agcctcgctt
    12421 taggagaacg cagaatgagt cgggcgcagc tgatcaacat ggccaggcac aacatcggcc
    12481 acgcgaaagc cggaaccatc gagcaggctc cggacatcgt caaggtgccg gtgcagaact
    12541 acttcgaccc cgatcgctgg cagctggaga tggatcgcat cttccgccgc atgccgctgg
    12601 tgctggcgat gacggacgag ctgcgcaatc cgggcgacta caaagccatg gaagcggtgg
    12661 gcatgcccgt gctcttgagc cgcggcgagg acggcgaggt ccgggcattc gtcaacatgt
    12721 gccgacatcg cggatcccag gtgatgctgc ccggatgcgg caacgctcat cgtttcacct
    12781 gtccctacca tgcatggacc tacgaccagg agggagctct ggtcggcgtc tacgcgccga
    12841 aggatttcgg cgacgtcgac aaggaggaaa acggcctcgt gccccttccc gtcgccgagc
    12901 gggcgggcat gatctgggtc atcgtcaatc cccaggcgac cctggacatc gacaccttcc
    12961 tgtccggata cgacgagctg ctcgggcact ttcacttcga ggactggcat ctcttcgagc
    13021 agcgcacgat cgccggcccc aactggaaga tcgcctacga cggctacatg gacctctacc
    13081 acctgccgat cctgcacaag gacacgttcg gtgatcagtt tcccaaccag gcgctgtact
    13141 acgcctgggg gccccatcag cgggtcagct cgccggaccc gacgcttgca ggctacgagc
    13201 agctgccgga gtccgagtgg aacgaagcac acatgctggc gggcgtgtgg accatctttc
    13261 cgcacgtctc cgtcgccggc ttcgacggcg gcggccgcag cgtcatggtg tcccagctgt
    13321 tcccgggcaa gacgccccag gaatcggtga ccgtgcagaa ctacctgatg gaaaaggcgc
    13381 ccgacgccga gcaggctgag gcggccgagc agatgttcaa gctgctcgag tacgtggtgc
    13441 aggaggagga ctacgccacc ggcctgcggc agcagaaagc aatcatgacc ggcgccatga
    13501 gccacgtgat gttcggccgg aacgaagagg gcgggcagcg cttccaccgc tggctggaca
    13561 ccctgctcgc aaccgatgac gaggacctca acgccctgtt tgcgggcggc gagcgttact
    13621 gctgaccgcg ggcttcccgg acggcctccc gggcgccgaa gagccccggc gagaggcgcg
    13681 tcatgcggcg ccaggggccc tgcaccattg gtgacatccg cgcaaacggt gttatattcc
    13741 agcgctgccc gtccggcccg gccgccagac aacggtcgac gccggcgtcc tgggggacgc
    13801 atggagcagg gggggcctga aacgaggccc agggacacag ggatcccgat tcgcagagag
    13861 ctctcaaaga aagggcccca gcgcatgaat gcgaacgttc ggcgcatact gatcagcaac
    13921 ggcaaaggcg ggtgcggcaa gaccaccatc gccgccaatc ttgccgtgtc atacgccaat
    13981 cgcggcgcca aggtcgcact ggtcgaccag gatccgcagg cgtccagcag ctattgggcg
    14041 gaccagcgct cccgggagct tgcacccgtt cacctcgtcc gcgcgcatca gcgcgccaac
    14101 atgtatcaga ctcagacctt tcacaaccgg atacccgccg acatcagccg ggtcgtcgtc
    14161 gacggcagca ccaacccaca ggacagagaa ctggagatgc tcctgaagca gtgcgacgtg
    14221 attctggttc cgctgctgcc gtcggccatc gacattcgag ccggcagcca gttcatcaag
    14281 gagctgatca cccaccggag cttccggacg ctgcccaagc ccgtgggcgt catcgccaac
    14341 cgggttcagc cgaactccac catgcacgcg cggctggagc acttcctcaa gtgcctggac
    14401 gttccgtggg tcgccacctt ccgtgacagc cccctctatc cggaagcctt cgagcgggga
    14461 gagggcgtcg tggacctgat cgactgccgg gccgcgcgcc gggaagcgcc cgcgtggcga
    14521 gcgctcacgg cctggatcga gcggcagccc gttagccgag gttgcacggc ccgggagctg
    14581 cgcaaccgac cgatccgggc gccgcgccac gtcgacgacg agaccttgag cgcatagaat
    14641 ccggcgccag ccagcttctc gggtcttccg aaaatcaggc ccggcgccac ctcatctgat
    14701 cacgttctgc gcgtcatttt cacagagccg ccccaccgat gctccagctg atcgacattt
    14761 ccttgaagcg cggctcaaac cagctgttcg agcacctgac gtgcacgatc cacccaggcc
    14821 acaaggtcgg catggtcggg cgcaacggcg cgggcaaatc aactctgttc gacatgatcc
    14881 tcggccgcct gcagcccgac gagggagagg tgagccgccc ctctggctgg gtagcctccc
    14941 acatggccca ggaagtcaac gtcaccgatc ggccggccat cgagcacgtg atcgacggcc
    15001 accgggagct ccgagccgtt gagcgccgca tcgcacaggc cgagtcccgg cgcgatgacc
    15061 tgacgctggc ggagctgcat acgcgcttcg gcgacgtcgg cgggcaccag gcacgggccc
    15121 gcgccgggac tatcctgcac gggctcggct ttgccgccga tgagatcgag cagccgtttc
    15181 gagctttctc cggcggcatg cggatccgcc tgaatctggc ccaggcgctg atggctccgg
    15241 ccgatctgct gcttctggac gagcctacca accacctgga cctggagacc acggtctggc
    15301 tggaatcctg gctgcagcgc tttgctggaa ccctgctcgt gatcgcgcac gatcgggcct
    15361 tcctcgacgg cgtgtgcgac cacatcgttc acatcgaaca cgggcgcgcg aactcctacc
    15421 ggggcaacta ctcctcgttc gagcggcaac gagcggaagc gctggcgcag cagcaggcgg
    15481 cccacgccag gcagcaggcc gagatcaagc acatgcagca tttcatcgac cggtttcgcg
    15541 ccaaggccag caaagcgaag caggcacaga gccgaatgag ggccctggag cgcatgcagg
    15601 ccgtagcccc cgtgcatgcc gactcgccct accgcttcag cttcgcgaat ccggagcaga
    15661 tgtcccaccc cctgctgagc ctggaggacg tgtccgtggg ctacgaagga cgcgcggtga
    15721 tcgagcacgt caggcagacg cttctgcccg gcgcccgcat cggcatactg ggagccaacg
    15781 gcgccggcaa gtccaccctg ctcaagtgtc tggtgggcga gctcgagccc ctggcgggca
    15841 cgctggtgcg cggacgccac tccgcggtcg ggtacttcgc ccagcatcag ctcgaacggc
    15901 tggacgggca gacgtccgct ctggcgaccc tcacgaacga gcggccacag gcccgggagc
    15961 aatggtgccg ggactacctg ggccgctggg gcttccccgc cgacatggtg gagcggccca
    16021 tcgttacgct ttccggcggc gagaaagccc gtctcgtgct ggccctgatc gctctcgccc
    16081 agccggccat tctcgtcctc gacgagccga ccaaccacct ggacctggaa atgcgcgagg
    16141 ccctggccct ggcgttgcag gactatgcgg gcgccctgct gctggtctcc cacgatcgca
    16201 gcctgctgcg gcgggccgtg gacgagttct ggctggttga ggggggcagg cttgcgagtt
    16261 tcgccggcga tatcgacgcc tacgccgaga gccgtctgag gcgaagcgaa cctccgtcat
    16321 ccgaacgcgc tcccgagacg aaccgcaaag ccctgcgaca ggcggcggcc gcccagcggg
    16381 aggcggccaa gccgttgcgg gcaaagctca ggcacctgga aaaggagatg gatcagatcg
    16441 gcgtggagct gcgcgaggtg gagtcccgac tggcggatgg ggacgtgtac cggagcctgc
    16501 ctccggagga gctcgacgac ctgctctccg ctgccggcag gctgagaaaa agattggacg
    16561 cgaccgagca ggcctggctc agcgcaaccg aggcgctcga agcgctcgag cagagctgaa
    16621 cggcgcgccc ggaggggatg agggtcggtc cggacgccta gccgcccgcc gaatcgtcgg
    16681 cggctgcttt tttagagcct ttctcgtcca cgaccacctc ctcgtccacg acgatccgaa
    16741 cgtcaactcc atcgtcgccg tcgatgccac gccgaatccg gatgatgctc ggatcgatcg
    16801 tgatggccat gtgatcgccc gcggagaact gcgagcagtc gatggacacc tcgcgtttct
    16861 tgccgttgcg cagaaccccg gcgtcgatgg tcccgtcact catgg
//
LOCUS       DBNHOX010000628         5449 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1336607, whole genome shotgun sequence.
ACCESSION   DBNHOX010000628 DBNHOX010000000
VERSION     DBNHOX010000628.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1336607"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /gene="sdhA"
                     /locus_tag="AC16UZ_13845"
     CDS             <1..1473
                     /gene="sdhA"
                     /locus_tag="AC16UZ_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HIR6247815.1"
                     /translation="LEHMGLPFSRTEQGRIYQRPFGGQSKDFGKGGQAARTCAAADRT
                     GHTLLHTLYQANLRANTTFLNEWFAVDLVKNQDGAVVGIIAIEMETGETVFVSSKATV
                     LATGGAGRIYASTTNAHMCTGDGAGMALRAGFPMQDMEMWQFHPTGIAGAGTLVTEGC
                     RGEGGYLINKDGERFMERYAPTAKDLAGRDVVARSMILEILDGRGCGPDRDHVMLKLD
                     HLGEEVLNSRLPGILELSRTFAHVDPVKEPIPVVPTCHYMMGGIPTGVLGQALTQDAQ
                     GDDREIPGLYAAGEAACVSVHGANRLGGNSLLDLIVFGRAAGLHIEEVMRQGVSYRDP
                     SDSDLEQAQARLNRWDESGPGETVADLKKELQTTMQNSFGVFRTGTHMEEGIKKIAEL
                     RDRISRAHLGDKSKVFNTARLEALELDNLLEVAEATAIAANARTESRGAHAREDFQVR
                     DDENWLCHSLFFSDERRVTKRAVNFSPETVDTFQPMERKY"
     gene            1482..2189
                     /locus_tag="AC16UZ_13850"
     CDS             1482..2189
                     /locus_tag="AC16UZ_13850"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIR6247816.1"
                     /translation="MMQVSVYRYNPETDEVPAMREMSIELPGDRDLMVLDVLEMLKAE
                     DPTLSYRRSCREGVCGSDGINMNGRNGLACITPVSSVAKRGKLVLRPLPGLPVIRDLV
                     VDMTQFYRQYEKIKPYLINDKPPPAQERLQSPEERAELDGLYECILCACCSTACPSFW
                     WNPDKFIGPAGLLQAYRFLADSRDTATAERLSELDDPFSVFRCRGIMNCVSVCPKGLN
                     PTRAIGKIRTMLLQQGT"
     gene            2278..5115
                     /locus_tag="AC16UZ_13855"
     CDS             2278..5115
                     /locus_tag="AC16UZ_13855"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018925108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIR6247817.1"
                     /translation="MADSFRERMERSSHLAGANAAYIEAMYERYLDEPNEVPPEWREY
                     FDTLPRVEGAVGADVPHSTIVDQFERLGRNRLKARPERESHFAQNVHEQKQMRVQDLI
                     SAYRHRGHKKANIDPLGMMERPAMPILDLAYHHLSLADLDETFNVGSFHYGSPEAKLR
                     DLVDALERTYCASIGFEYMHIVDQSEQLWVQQRLESVRARPVLTEDHKRVILERLTAA
                     EGLEKYLHGRYPGTKRFGLEGGESLIPMLHELLQRLGSHGVLEAVIAMAHRGRLNVLV
                     NILGKDPRLLFDEFDGKLPVNEDDMGDVKYHQGFSSNVMTPGGEMHLALAFNPSHLEI
                     VGPVVEGSVRARQDRRRDHVGTRVVPILVHGDASFAGQGVVMETLQMSQTRGFKTGGT
                     IHIIINNQIGFTTHRQDDARSTEYCSEVAKLVQAPIFHVNGDDPEAVLFASQLAADYR
                     MEFKKDVVIDLVCYRRRGHNEAEEPMKTQPLMYKKIRQHPTTRAIYVERLIGEGLVED
                     SEADGLAEAYRNTLDQGGHVALSLVKEPNTELFVDWTPYLGHDTVMPADTRVDLSVIQ
                     ALARRLEELPDGFVLQRQVQKIMEDRHKMSFGAMPINWGFAETMAYATLLDEGYGVRL
                     TGQDVGVGTFSHRHAALHNQKDGKRYIPLNSLSETVNFDLYDSLLSEEAVLAFEYGYA
                     ATAPDKLVIWEAQFGDFANGAQVVIDQFISSGEVKWARLCGLTMLLPHGYEGAGPEHS
                     SARLERFLQLCAQHNMQVCLPTTPAQVFHMLRRQVVWPARKPLVALTPKSLLRHKLAV
                     STLEDLANGTFHTVLGEVDEIDPDGVERVVLCSGKVFYELLEARREANASQVAIIRLE
                     QLYPFPEAELERIMAPYGSVRDVVWCQEEPMNQGAWYASQHHMRRVIMKHNENIYLAY
                     AGRPAFAAPASGHAHLHGVRQQRLVEEALFG"
     gene            5136..>5449
                     /locus_tag="AC16UZ_13860"
     CDS             5136..>5449
                     /locus_tag="AC16UZ_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR6247818.1"
                     /translation="MTIQIKAPTFPESIADGTVAAWHRNVGDQVQRDDLLVEIETDKV
                     VLEVVAPAAGRLTSVLKAEGETVLSEELIAELDETAAGEPKQEVEADEPQQAQQPEPA
                     DD"
BASE COUNT         1068 a         1708 c         1751 g          922 t
ORIGIN      
        1 ctcgagcaca tggggctgcc gttttcccga accgagcagg gccgcatcta tcagcggccc
       61 tttggcgggc agtcgaagga tttcggcaag ggcggccagg cggcccgaac ctgcgcggcc
      121 gctgacagga cgggtcatac ccttctgcat acgctctacc aggccaacct gcgggcaaac
      181 accacatttc tgaacgaatg gttcgccgtg gatctggtca agaaccagga cggcgccgtc
      241 gtcggcatca tcgccatcga gatggagacc ggagaaacgg tcttcgtcag ctccaaggcc
      301 accgtgctgg ccacgggagg cgcgggccgg atctacgcga gcaccaccaa cgctcacatg
      361 tgcaccggcg atggagccgg aatggcgctc agggccggtt tccccatgca ggacatggag
      421 atgtggcagt tccatcccac cggaatcgcc ggggccggta ccctggtgac cgagggttgc
      481 cgcggcgaag gcggctacct catcaacaag gacggcgaac gcttcatgga gcgctacgcg
      541 cctactgcga aggatctcgc gggccgcgac gtggtggccc ggtcaatgat cctggagatt
      601 ctcgacggtc gcggttgtgg cccggacagg gaccacgtga tgctcaagct cgatcatctg
      661 ggagaagagg tgctcaacag ccgcctgccg ggaatcctcg agctgtcccg cacatttgct
      721 catgtggatc ccgtcaagga gccgatcccc gtagttccca cctgccatta catgatgggc
      781 ggtattccca ccggcgtgct cggccaggcg cttacccagg acgcccaagg cgacgaccgc
      841 gaaatccccg gtctttatgc tgctggagag gccgcatgcg tctccgtgca tggcgccaac
      901 cgactcggcg gcaactcgct gctcgacctc atcgtgttcg gtcgggccgc ggggctgcac
      961 atagaggaag tgatgcgtca gggcgtttcc tatcgggatc ccagcgacag tgatctcgag
     1021 caggctcagg cccgtctgaa ccgctgggac gaaagtggtc cgggcgaaac ggtcgccgac
     1081 ctcaagaagg agctgcagac gacgatgcag aacagcttcg gcgtgttccg gaccgggact
     1141 cacatggaag agggcatcaa gaagattgcg gagctgcgcg atcgcatctc tcgagcccat
     1201 ctcggcgaca agagcaaggt cttcaacacg gcgcggctag aagcccttga gctggacaac
     1261 ctgctcgagg tggcagaggc caccgccatt gccgccaacg cccgcacgga gagccgaggc
     1321 gctcacgccc gggaggactt ccaggtgcgc gacgacgaga actggctttg ccactcgctg
     1381 tttttctcgg acgaaagacg ggttaccaag cgagccgtga acttcagccc tgagaccgtg
     1441 gacaccttcc agcccatgga acggaagtac tagagaggat ggtgatgcag gttagcgttt
     1501 atcgatacaa cccggagacc gacgaggtcc cggccatgcg cgagatgagc atcgagctcc
     1561 ccggcgaccg ggatctgatg gttctcgacg ttctcgagat gctgaaggcc gaggatccta
     1621 ccctgtccta ccggcgctcc tgccgggagg gtgtttgcgg ttcggacggc atcaacatga
     1681 acgggcgaaa cgggctcgcc tgcatcacgc cggtttcctc tgttgcaaag cggggcaagc
     1741 tcgttctgcg gccgcttccc gggctgccgg tgatccggga cctggtggtg gacatgactc
     1801 agttctatcg ccagtacgaa aagatcaagc cgtacctcat taacgacaag ccgccccctg
     1861 cccaggagcg cctgcagagt ccggaggagc gtgccgagct cgacgggttg tacgagtgca
     1921 tcctgtgtgc ctgctgctcc accgcctgcc cctcgttctg gtggaaccct gacaagttca
     1981 tcggtccggc gggtctgttg caggcctatc ggttcctggc cgacagccgg gacacggcga
     2041 cagccgagcg gctctcggag ctggacgacc ccttcagcgt cttccgatgc cgcgggatca
     2101 tgaactgcgt gagcgtttgc cccaaggggc tcaaccccac gcgggccatc ggcaagatcc
     2161 gcaccatgct tctgcagcag gggacgtaga cgaacccctt gcgcggctat actgctgctt
     2221 caacggacaa aaggctgacg gctctcacgg tgagctccta tcccacgcag gctgacaatg
     2281 gctgactcgt ttcgcgagcg catggagcgt agttcgcacc tggccggtgc caacgctgcc
     2341 tacatcgagg ccatgtacga gcgctatctc gacgaaccga acgaggtgcc ccctgagtgg
     2401 cgcgagtatt tcgacacgct gccgcgcgtg gaaggggctg tcggtgccga tgtgccccac
     2461 agcaccatcg tcgatcagtt cgagcggctg ggacgcaacc gcctgaaagc gagaccggag
     2521 cgcgagagcc actttgcgca gaacgtgcac gagcagaagc agatgcgcgt tcaggatctc
     2581 atctcggcct atcggcaccg gggccacaag aaggcgaaca tcgacccgct gggcatgatg
     2641 gaacgacccg ccatgcccat cctcgatctg gcctatcacc atctctccct cgcggatctg
     2701 gacgagacgt tcaacgttgg atcttttcac tacggatcac cagaggcgaa gctgcgcgat
     2761 ctcgtggatg ccctcgagcg gacctactgc gcgtccatcg gcttcgagta catgcacatc
     2821 gtcgatcaga gcgagcagct ctgggttcag cagcgcctcg aaagcgtgcg tgcgcggccg
     2881 gtactgaccg aggatcacaa gcgggtgatc ctggaacgcc tgaccgctgc agaaggcctg
     2941 gaaaagtacc tgcacggccg ttatcccggt accaagcgtt tcggcctgga aggcggggag
     3001 agcctcatcc ccatgcttca cgagctgctg cagcggctgg gttcccatgg cgtcctcgag
     3061 gccgtcatcg ccatggcgca tcggggccgg ctcaacgtgc tggtcaacat cctgggaaaa
     3121 gacccccggc tccttttcga tgagttcgac ggcaagctgc cggtgaacga agacgacatg
     3181 ggagacgtca agtaccacca gggattttcc agcaacgtga tgacgccggg cggcgagatg
     3241 cacctggcgc tggcgttcaa tccttcccac ctggagatcg tcgggcccgt ggtggaggga
     3301 tccgtgcggg cgaggcagga tcgccgccgc gaccatgtgg gaacccgggt cgtgcccatc
     3361 ctcgttcacg gcgacgcgtc cttcgccggg cagggagtgg tcatggagac cctgcagatg
     3421 tctcagaccc gagggttcaa gacgggcggg acgatccata tcatcatcaa caaccagatc
     3481 ggcttcacca cccatcgtca ggacgacgcg cgctccaccg agtactgctc ggaggtggcc
     3541 aagctggttc aggcccccat cttccacgtc aacggcgacg atccggaggc ggtgctgttt
     3601 gcttcgcaac ttgccgccga ttaccggatg gagttcaaaa aggacgtggt gatcgatctc
     3661 gtctgctatc gccgcagggg tcacaacgag gccgaagagc ccatgaagac ccagccgctg
     3721 atgtacaaga agatccgtca gcacccgacc acccgggcga tatacgtgga acgcctgata
     3781 ggggaagggc tcgtggagga cagcgaggcc gatggccttg ccgaggcgta ccggaacacc
     3841 ctggatcagg gagggcacgt ggccctgagc ctggtgaagg aaccgaacac cgagctgttc
     3901 gtggactgga cgccctatct gggccacgac accgtcatgc ccgcggatac ccgcgtcgat
     3961 ctcagcgtga tacaggcgct cgccaggcgg ctcgaggagc tccccgatgg tttcgtgctt
     4021 cagcgccagg tccagaagat catggaagat cgacacaaga tgagcttcgg ggcgatgccc
     4081 atcaactggg gatttgccga gaccatggcg tacgcaacgc tgctggacga gggttacgga
     4141 gtccgcctga cgggccagga cgtgggcgtc gggacctttt ctcaccggca tgccgctctc
     4201 cacaaccaga aggacggcaa gcgctacatc cccctgaaca gcctctctga gaccgtcaac
     4261 ttcgacctct acgacagcct gctttcggaa gaggcggtgc tcgccttcga gtacggttac
     4321 gccgcgaccg cgccggacaa gcttgtcatc tgggaggccc agttcggtga ctttgccaat
     4381 ggtgcccagg tggtcatcga ccagttcatc tccagcggcg aggtgaaatg ggcccggctg
     4441 tgcggtttga ccatgctgct gccccacggc tatgagggcg ccggacccga gcactcgtcg
     4501 gcgaggctcg agcggtttct ccagctctgc gcccaacaca acatgcaggt ctgccttccc
     4561 accacgccgg ctcaggtgtt tcacatgttg cgtcgtcagg tggtctggcc cgcgcgcaag
     4621 ccgctggtgg cgctcacgcc aaagagcctg ctcaggcaca agctcgccgt gtcgacgctc
     4681 gaggacctgg cgaacgggac gtttcacacg gtgctgggcg aagtcgacga gatagatccg
     4741 gacggcgtcg agcgcgttgt gctctgcagc gggaaggttt tctacgagct gctggaagcc
     4801 cgtcgggagg cgaacgcgag tcaggtcgcc atcatcaggc tcgagcagct ctacccgttt
     4861 cccgaggcgg agctcgagcg catcatggcg ccgtacggct cggtacggga cgtggtctgg
     4921 tgtcaggagg aacccatgaa ccagggtgcc tggtacgcct cccagcacca tatgcgccgg
     4981 gtcatcatga agcacaatga aaacatctac ctggcctacg ccgggcgccc ggctttcgcc
     5041 gccccggcca gcgggcacgc gcatctgcac ggcgtgcggc agcagcgcct ggtggaggag
     5101 gccctgttcg gctaacgggc tatgaaggac cgccgatgac catccaaatc aaagcgccaa
     5161 cgtttccgga atccatcgcc gacgggaccg tagccgcctg gcaccggaac gtgggcgatc
     5221 aggtccagcg cgacgatctg ctggtggaaa tagagacgga caaggtggtg ctcgaggtgg
     5281 tggcacccgc ggcaggacgc cttaccagcg tcctgaaagc cgagggggaa acggtcctca
     5341 gcgaagagct cattgcggag ctcgacgaaa ccgcggcggg cgagcccaag caagaagtag
     5401 aggctgacga gccgcagcag gctcagcagc ccgagcccgc ggacgacga
//
LOCUS       DBNHOX010000629         2099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1015220, whole genome shotgun sequence.
ACCESSION   DBNHOX010000629 DBNHOX010000000
VERSION     DBNHOX010000629.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1015220"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="AC16UZ_13865"
     CDS             <1..761
                     /locus_tag="AC16UZ_13865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6247819.1"
                     /translation="TLYGAGAEPGNCPVWDGSQGDNTWGAEYNGDYTCHWTTCRDSVG
                     QIAALLDEIEALYCVDRSMIWVTGCSNGGMMVYELAKDARTRTRFAGFMPQVGAPHPG
                     FRQYDPASELVDRPRHLLGFWGETDRTVPGRANFPGTSPQVALDTSFNGWLYVPASDI
                     GYEWASMHDAAPLPVEEADAYARAYSNSLDCQVWQAQDEGETPGHASEVATCFFQGGH
                     SCPGFGGMEAMMWDFAGRHAKTDADPAACSTSSP"
     gene            complement(773..1159)
                     /locus_tag="AC16UZ_13870"
     CDS             complement(773..1159)
                     /locus_tag="AC16UZ_13870"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6247820.1"
                     /translation="MIDINDRMAILDLCASYNYLFDTCAAKEWAATFTADGVFEGPAG
                     RAEGPEDLAEFCRNTNAQYPGAMHFTDNHLFEQDGDTVRHKCFLSMQVPSPEGTQTLL
                     LTYEDELRRVDGEWRFASRLCQPLSA"
     gene            complement(1223..>2099)
                     /locus_tag="AC16UZ_13875"
     CDS             complement(1223..>2099)
                     /locus_tag="AC16UZ_13875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6247821.1"
                     /translation="PDFPYDPRYAEWSGLRVAHIDEGPADAPVALLLHGEPTWSYLYR
                     KMIPPLLEAGYRCVAPDHVGFGRSDKPVEDDWYVIERHVERITFLIESLDLTDITLFC
                     QDWGGPTGLRQAVDMPERFERLVIMNTWLHHEGYEYSPAVRFWREAATNRAWLGWTGG
                     DLPCGAIVAGTLARSGLDRAAVKRAYDAPFAEGGRSKAGARRFPWCLPFGEPEAGNAA
                     DQARCFEALKSWAKPAHFIFGERDPIFTTGWARQWSALLPNATLDLIPDASHFVQEEA
                     GEEIVAAFLERTRST"
BASE COUNT          326 a          678 c          740 g          355 t
ORIGIN      
        1 ggaccctcta cggagcggga gccgagccgg ggaactgccc cgtctgggac ggcagtcagg
       61 gtgacaatac ctggggcgct gagtacaacg gcgactacac ctgccactgg accacctgtc
      121 gcgactcggt gggccagatc gcggcgctgc tcgacgagat cgaggcgctt tactgcgtgg
      181 accgcagcat gatctgggtg accggctgct ccaacggcgg gatgatggtc tacgagctcg
      241 ccaaggatgc gcgaacgcgc acccggtttg ccggcttcat gcctcaggtg ggcgcgcccc
      301 atccgggttt ccggcagtac gaccccgcaa gtgagctcgt ggaccggccc cgtcacctgc
      361 tggggttctg gggagagacg gaccggacgg tgcccggtcg tgccaacttt cctgggacgt
      421 cgccccaggt tgctctcgac acgagcttca acggctggct ttacgtgccg gcgagcgaca
      481 tcgggtacga gtgggcatcg atgcacgatg cggcgccgct gcctgtcgaa gaagccgacg
      541 cctacgcgcg cgcctactcg aacagtctcg attgccaggt ctggcaggcc caggacgagg
      601 gtgaaacgcc tggccatgcc tccgaggtcg cgacctgctt cttccagggg gggcactcct
      661 gtccgggctt tggcggcatg gaggcgatga tgtgggactt cgccggccgc cacgccaaga
      721 cggacgcgga tccggcggcg tgctccactt ccagccctta gtggtcgtca ggctatgcgc
      781 tcagcggctg gcacaggcgt gaggcgaagc gccactcgcc gtcgacgcgt ctcagctcgt
      841 cctcgtaggt gagcagtagg gtctgggtgc cctcggggga gggaacctgc atgctcagga
      901 agcacttgtg cctgaccgtg tcgccgtcct gctcgaagag gtggttgtcg gtgaagtgca
      961 tggcgcccgg gtactgcgcg ttggtgttcc ggcagaactc ggcgaggtcc tccggaccct
     1021 ccgcccgtcc ggccgggcct tcgaagacgc cgtcggcggt gaacgtggcc gcccactcct
     1081 tcgcggcgca ggtgtcgaag aggtagttgt agctcgcaca aaggtcgaga atggccatgc
     1141 ggtcgttgat gtcgatcatg agatctccct gaaggctcgt ctgggttcgt caggccaggc
     1201 catgctagac gcgcggtgcc gatcacgtcg agcgcgtgcg ctccagaaat gcggcgacga
     1261 tctcctcgcc ggcctcctcc tgcacgaagt ggctggcgtc ggggatcaga tcgagggtgg
     1321 cgttgggcag cagcgccgac cattgcctgg cccagcccgt ggtgaatatc gggtccctct
     1381 cgccgaaaat gaaatgggcg ggcttggccc agctttttag cgcttcgaag cagcgcgcct
     1441 gatcagcggc gttgccggcc tccggctcgc cgaatggaag gcaccatggg aaccggcgcg
     1501 cgcctgcctt ggagcggccg ccctcagcga atggcgcgtc gtaagcgcgc ttcacggccg
     1561 ctcggtccag cccgctgcgg gccagcgtgc ccgcgacgat ggcgccgcag ggcaggtcgc
     1621 cgcccgtcca gccgagccag gcccggttcg tggcggcctc ccgccagaag cggacggccg
     1681 gtgagtactc gtagccttcg tgatgcagcc aggtgttcat gatcaccagc cgctcgaagc
     1741 gttcgggcat gtccacggcc tgtcttaagc ccgtggggcc gccccagtcc tgacagaaca
     1801 gggtgatgtc cgtgaggtcc agggactcga tgaggaaggt gatccgctcc acgtgccgct
     1861 cgatcacgta ccagtcgtcc tccacgggct tgtcagagcg gccgaacccc acgtgatcgg
     1921 gggccacgca gcgatagccg gcctccagca gcggcgggat catcttgcgg tacaggtagc
     1981 tccaggtcgg ctcgccgtgc agcagcagcg cgaccggggc gtccgcgggg ccctcgtcga
     2041 tgtgggcgac ccgcaggccg ctccactcag cgtagcgagg gtcgtaggga aagtcgggg
//
LOCUS       DBNHOX010000630         2662 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1785234, whole genome shotgun sequence.
ACCESSION   DBNHOX010000630 DBNHOX010000000
VERSION     DBNHOX010000630.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1785234"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /gene="bamA"
                     /locus_tag="AC16UZ_13880"
     CDS             complement(<1..1174)
                     /gene="bamA"
                     /locus_tag="AC16UZ_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HIR6247822.1"
                     /translation="MTGRGARIVVLLALLGSASLARADAFTISDIRLQGLQRVSAGTV
                     FNVLPVSVGDTIDEVSVRQLVRLLFRSGYFDDIRMARDGDILVVTFKERPAIESIEID
                     GNKAIKTDALLDGLGQQGLREGEIFKQATLERVGLELERQYVSQGRYGAAIDTQVEQL
                     PRNRVAIKIEIEEGKTSGIRHINIVGDEVFEQEELLDQLELQHPSLFSFYRNDDKYAR
                     EKLTADLEKLESYYRDRGYVEFDIESTQVSITPDRRQVYITVNIEEGDKYTVSGVNLI
                     GELSDVNPDDLKRLFLVAEGQVFSQALVTATEERLTQALGNSGYTFASASGVPRVSED
                     GTVEVVFNVDSGKRAYVRRVSFAGNKVTQDEVMRREMRQMEGGWASTAQIDFSKVRL"
     gene            complement(1179..2513)
                     /gene="rseP"
                     /locus_tag="AC16UZ_13885"
     CDS             complement(1179..2513)
                     /gene="rseP"
                     /locus_tag="AC16UZ_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016485213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR6247823.1"
                     /translation="MDFLQYPLALLVTLGVLVTFHELGHFVIARWSGVTVVRFSVGFG
                     KPFWSWRDRRGTEFALAYIPLGGYVRMLDEREPGVIEYDRRSTDVSYNELSVWWRMAI
                     ALGGPAANFLLAIIVYWVLFVIGTITYAPLLGSVGADSAIGRAGLREHEELIAVDGVE
                     TSSWQAVNMALAGRLGESGEIELTTRRPGSDSVRSGAVPISSWHTGVDEPDLLGSLGI
                     VPALPPIVAQVVPDSPAERAGVRRWDRIVSVDGEAVADWGQWVDLIRNAPDRRLSVTV
                     DRSGQVLVLDVTPDVQEAAGGEVTGFLGVGAYTKEIRYGVLAAVPKSLWESWDKTALT
                     LGLLKKMITGHVSVKNLSGPITIAKVAGDSARSGWRFFFSVLALLSISLGVLNLLPIP
                     ILDGGHILFCGAEAVTGRPVSERVQILGTQIGLFMVASLMLLAIYNDISRLL"
BASE COUNT          510 a          897 c          809 g          446 t
ORIGIN      
        1 cgagccggac cttggagaag tcgatctgcg ccgtggaagc ccagccccct tccatctgcc
       61 gcatctctct gcgcatcacc tcgtcctggg tgaccttgtt gccggcaaag gaaacacggc
      121 gcacgtatgc ccgcttgccg gaatccacgt tgaacacgac ctcgaccgtg ccgtcctcgc
      181 tgacgcgagg tacgcccgag gcggatgcga acgtgtaacc ggagttgccg agggcttggg
      241 tgagccgttc ctccgttgcg gtgacgagag cctgagagaa aacctgcccc tcggccacga
      301 ggaacagacg cttgaggtcg tcggggttca cgtcgctgag ctcgccgatc aggttgacgc
      361 cgctgaccgt gtacttgtcg ccttcctcta tgttcaccgt gatgtagacc tggcgccggt
      421 ccggcgtgat gctcacctgg gtcgactcga tgtcgaactc cacatagccg cgatcgcggt
      481 agtaggactc cagcttctcc agatcggccg tgagcttctc ccgcgcgtac ttgtcgtcgt
      541 tgcggtagaa ggaaaagagg ctcgggtgct gcaactccag ctgatcgagc agctcttcct
      601 gctcgaagac ctcgtcgccg acgatgttga tgtgccggat gccggaagtc tttccttcct
      661 cgatttcgat cttgatggcc acacgattcc ggggcagctg ctcaacctgg gtgtcgatgg
      721 cggcgccgta acggccctga gaaacgtact gccgctccag ctccagcccg acccgctcca
      781 gggtcgcctg cttgaagatt tccccctccc gcagtccctg ctggcccagc ccgtcgagga
      841 gcgcgtccgt cttgatcgcc ttgttgccgt cgatctcgat gctctcgatg gccggccgtt
      901 ccttgaacgt gaccaccagg atgtcgccgt cgcgagccat gcggatgtcg tcgaaatagc
      961 ccgacctgaa cagcaggcgt accagctggc ggacgctcac ctcgtcgatg gtgtccccga
     1021 cgctcacggg gagcacgttg aaaaccgtcc ccgcggacac ccgctgaaga ccctgcaaac
     1081 gtatgtccga tatggtgaag gcgtctgccc gagccagact tgccgacccg agcagggcca
     1141 gcagcacaac gattcgcgct cccctccccg tcatcgtttc agagcagcct ggagatgtcg
     1201 ttgtagatcg ccagcagcat caggctggcc accatgaaca ggccgatctg ggtccccaga
     1261 atctgaaccc gctcggagac cggccggccg gtaaccgcct ccgccccgca aaacaggatg
     1321 tggcccccgt cgagaatggg aatgggcagc aggttgagca cgcccagcga gatgctgagc
     1381 agcgcgagaa cgctgaagaa gaagcgccac cccgagcggg ccgagtcacc ggccaccttc
     1441 gcgatggtga tgggcccact gagattcttg acggacacgt gcccggtgat catcttcttg
     1501 agcaggccaa gcgtcagcgc ggtcttgtcc caggactccc agaggctctt gggaacggca
     1561 gcaagcacgc cgtaccggat ctccttggtg tacgcgccca ccccgaggaa gccggtcacc
     1621 tcgccgccgg cagcttcctg gacgtccggc gtcacgtcca gcaccagcac ctgtccggaa
     1681 cggtctacgg tcacgctcaa gcgccggtcc ggggcattgc gtatcagatc cacccattgg
     1741 ccccagtcgg cgaccgcctc tccatcaaca gagacgatcc gatcccagcg acggaccccg
     1801 gcgcgctctg ccgggctgtc cggcaccacc tgggcgacga tggggggcag tgcgggcacg
     1861 atcccgagcg agccgagcag atccggttcg tcaacaccgg tgtgccagct ggaaatcggc
     1921 acggcaccgg agcgtaccga atccgatccg ggtctccggg tagtgagctc gatctcgccg
     1981 ctctcgccca gccgccccgc gagcgccatg ttcaccgcct gccaggagga cgtctcgacg
     2041 ccgtccacgg cgatgagctc ctcgtgctcg cgcagccccg ccctgccgat ggcggaatcc
     2101 gcccctacgc tgcccagaag cggggcgtag gtgatcgtac cgatcacaaa gagcacccag
     2161 tagacgatga tggccagcag gaaattggca gccggcccgc ccaaagcgat ggccatgcgc
     2221 caccagacgc tgagctcgtt gtaggagacg tccgtgctgc ggcggtcgta ctcgatgacg
     2281 ccaggctccc gctcgtcgag cattctcacg tagccgccca gcggaatgta cgcaagagcg
     2341 aactcggtgc cgcgccggtc gcgccacgac cagaaaggtt tcccgaagcc gaccgagaag
     2401 cggacgacgg tgacgcccga ccagcgcgcg atgacgaaat gacccagttc gtggaaagtc
     2461 accaggacgc cgagagtcac cagcagggca aggggatatt gcagaaaatc cataaactga
     2521 gtgcgacaga tgccccgtat caaaacttca gacagcagcg gtgctcgccg gttcgccccg
     2581 ccctggaatt acccgaccgt cgtaaagacc atcggcgagc gcatcataat ggatcagcga
     2641 gcgagaatca gcgcgaaaac cg
//
LOCUS       DBNHOX010000631         6942 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_50215, whole genome shotgun sequence.
ACCESSION   DBNHOX010000631 DBNHOX010000000
VERSION     DBNHOX010000631.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6942)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6942)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6942
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_50215"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="AC16UZ_13890"
     CDS             <1..633
                     /locus_tag="AC16UZ_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR6247824.1"
                     /translation="RLIGRITIDDVVDVIIEDADEAVLARAGLDLEDDTFAPIMKSVR
                     GRALWLGVNLLTTFVAAAVINVFEETIAKVVALAVLMPIVASMGGIAGTQTLTLVIRG
                     IAVGQLGRANLPYLLNREFLVAALNGLMLAIVVAVAAAVAFGDTVLGLVIAIAMLINL
                     LVAAVSGTLLPSILNALRIDPAIAGGVVLTTITDVTGFFTFLGLATWAYA"
     gene            complement(651..1997)
                     /gene="pmbA"
                     /locus_tag="AC16UZ_13895"
     CDS             complement(651..1997)
                     /gene="pmbA"
                     /locus_tag="AC16UZ_13895"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HIR6247825.1"
                     /translation="MPNELIDLQDSEAGLSSLVGDILDEARRQGADAAEVSVNEDAGL
                     SVSVRKGELETVEFNQDRGFGITVYYGLRKGSASTSDSSLTAIEDTVRAARDIARHTE
                     EDPCNGLADAGLMPDALPELDLYHPWRLDVGEAERLAIACEAAGLSRDERIVNSDGAQ
                     VSTQQSCRVYGNSHGFIGSYLGTRHSVSCVLIAADESGMQRDYWYSLARDPEGLEDME
                     SVGVEAARRVVARLSPRKPLTGRFPVVFSAQMSAGLVGHLVGALSGGSLYRNASFLLD
                     SLGHGVAAPHLSVLEEPHLPGRIGSSGFDGDGVATSAKHFVEDGVVRSYVLSTYSGRK
                     LGMPTTGNAGGVFNLLLRGRTLAVDDLYRSMGSGLLVTELMGQGVNLVTGDYSRGAAG
                     FWVENGAIAYPVHEVTIAANLKDMLLDVQALGDDVDERGNVRTPSVLVGSMMVAGS"
     gene            2039..2524
                     /gene="yjgA"
                     /locus_tag="AC16UZ_13900"
     CDS             2039..2524
                     /gene="yjgA"
                     /locus_tag="AC16UZ_13900"
                     /inference="COORDINATES: protein motif:HMM:NF016626.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HIR6247826.1"
                     /translation="MTEPPISKSERKRAAARLQEIGRALAELRPARLDEIPLPETLER
                     AIYDYRRFASREAKRRQLQRIGKLMREIDTAPIEAALERLEGRSASARYEQHQLERWR
                     DRLIEDPAALTDYLAEHPHSERQTLRHLIERARTAGNEQQQKTASRALFRFLREDVPT
                     G"
     gene            complement(2571..4013)
                     /gene="tldD"
                     /locus_tag="AC16UZ_13905"
     CDS             complement(2571..4013)
                     /gene="tldD"
                     /locus_tag="AC16UZ_13905"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017130658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HIR6247827.1"
                     /translation="MTDLLEEAQGQLWAPAGLEQSDLDRLMGRLSSRSIDVGELFFQH
                     QRSESWALEDGIVKDGSFSVDRGVGVRAISGEKTGFAYAEDIILPALTSATEAARSIA
                     RHGEDRSVPAWQVPRVPARYPSVDPISTLDGSEKVALLRRVDEAARQADPRVKEVNVR
                     LSASHESILVMASDGTLCADVRPLVRLDVSVIVEQDGRRERGSAGAGGRGDLGLVSDS
                     EFTDGLAREAVRTALVNLEAVDAPAGPMQVVLGHGWPGVLLHEAVGHGLEGDFNRKGS
                     SAFANRVGEKVASELCTVVDDGTLDGRRGSLTVDDEGSPSQETVLIENGVLRGYMQDK
                     LNARLMKTSSTGNGRRQSFAHLPMPRMTNTFMKAGEHDPEEIIASVDRGIYAVNFGGG
                     QVDITSGRFVFSATEAYLIEKGRVTHPVRGATLIGNGPEVMTRISMVGNDLELDRGIG
                     VCGKDGQSVPVGVGQPTLKIDEVTVGGTSA"
     gene            complement(4023..>6942)
                     /locus_tag="AC16UZ_13910"
     CDS             complement(4023..>6942)
                     /locus_tag="AC16UZ_13910"
                     /inference="COORDINATES: protein motif:HMM:NF024516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YhdP family phospholipid transporter"
                     /protein_id="HIR6247828.1"
                     /translation="AFGLVAHLDDSEEQVRTQRALRMPAVHVRKDAEVVQFWAQRVDM
                     GGVSEFLERALRGSERARRWLSALKLQAEALNVRGYYRLPTGESGFAATLAGLDVDGY
                     RGAPFVRGAAGELLGHARGLELKLKSEDVLLKFPQTFTDTWQMPYLQGAFQAWFGNGY
                     LGLRGVNLRAETLFSRAAGSFALTRPPGERYEERLSLLIGVDALDTDQAFTFVPYTID
                     EGLEDWLRGGLKAGLLTRIRFGYHGQQHLKVNELARRIELSARISDGRIAYAPDWPDV
                     HELDADVVVAGPMVRVTVDKGASEGVRLGGSQVVLRNNASYADVELVSRMETDLALDF
                     VRTTPLAESMGFVAPEWVGDGRLEVLGNLHIPIKLPEDVDASEQLQVDVNVRMLDAGL
                     RMPDFRLALGDLEGDLRYRYPNGLRSSRISGTLFGNDVLIDADSNSQEMILSFAGRAT
                     HEDVLAVLDMEDPGVVTGSADFLASLHVGVDPDHVTRLEVVSDLQGMALDLPADLRKP
                     AEVARPVEVAVQFLDERQIISFRHGTAQGWLHVADAPERGAVGFGQPPPVLDGRADYL
                     LLTGDLEGFALEEVVPDEEVGGSGFMPLKLSGLRVGQIRLDEFVIFDAVLDGDITAEG
                     FAITIDSEAVVGRVSMDGDAPLVLGLDRVVLPAGDGEGDPLSPQMAPELPEAVVEVGE
                     FRVGEADYGRWKFNVHPEPDGVRLADLEAVVRGVTVSSPEGVYWSTVDGESRFKGELA
                     AENLFDVLPQWGFAPSMETESAGMTGELSWAGSPANVDLDLLVGTASVRAETGSFVDV
                     ESGGGAVRMFSLINFDTIAKRMRGDFSDLTGKGLSFDKLKATVSFDRGHLAFVEPLKV
                     KGSGSEFEVAGNVDLVTGELDNEMIVTLPVSKSLPWYAAYIALANPLAGAGILVGERV
                     LRKPLEQFSSAKYEIGGTLDEPQVNLVSVFESEMVEAELESLAGAGDEDTFTELEPQE
                     "
BASE COUNT         1188 a         2475 c         2147 g         1132 t
ORIGIN      
        1 cgcctgatcg gccggatcac catcgacgac gtcgtcgacg tcatcatcga ggacgccgat
       61 gaagcggtgc tcgcccgggc cggtctggac ctggaggacg acaccttcgc gcccatcatg
      121 aagtccgtgc gcggccgcgc actgtggctc ggcgtcaacc tgctgaccac cttcgtggcc
      181 gcggcggtca tcaacgtgtt cgaggaaacc atcgccaagg tggtggcgct ggccgtgctg
      241 atgcccatcg tcgccagcat gggtggcatc gcgggcacgc agacgctgac gctggtcatt
      301 cgcggcattg ccgtggggca gctgggtcgc gccaacctgc cctatctgct caatcgggaa
      361 tttctggtcg ccgcgctgaa cggcttgatg ctcgccatcg tggtagccgt tgccgccgct
      421 gtcgccttcg gcgacaccgt gctgggcctg gtcatcgcca tcgccatgct gatcaacctg
      481 ctggtcgccg ccgtgtcggg aaccctgttg cccagcattc tcaacgccct gcggatcgat
      541 ccggccatcg ccggcggcgt ggtgctgacc acgatcaccg acgtgaccgg atttttcacc
      601 tttctcggtc tcgcgacctg ggcctacgcc tgacctggcg acaccccggt ttacgacccc
      661 gcgaccatca tggagccgac gagcacgctg ggcgtgcgca cgttcccgcg ttcgtccacg
      721 tcgtcgccca gcgcctgcac gtccagcagc atgtccttga ggttggcggc aatggtgacc
      781 tcgtgcaccg gataggcgat cgccccgttc tctacccaga accccgcggc accccgcgag
      841 tagtcaccag ttaccaggtt gacgccctga cccatcagct ccgtgaccag gagcccggag
      901 cccatggagc ggtaaaggtc gtccacggcc agcgttcggc ccctcagcag caggttgaaa
      961 acacccccgg cgttgcccgt cgtgggcatg ccgagcttcc gacccgagta ggtggacagg
     1021 acgtagctgc gcacgacgcc gtcctccacg aaatgcttcg ccgaggtggc caccccgtca
     1081 ccgtcgaaac ccgagctgcc gatgcggccg ggcaggtggg gttcctcgag gacgctcagg
     1141 tgaggcgcgg cgacgccgtg gcccagcgaa tcgaggagaa acgacgcgtt ccggtagaga
     1201 ctcccaccgc tcaacgcccc gaccaggtgc cccaccagac cggccgacat ctgcgccgag
     1261 aagacgacgg gaaagcggcc ggtcagcggc ttgcggggcg acagccgtgc caccaccctg
     1321 cgggcggctt cgacgcccac gctctccatg tcctccagcc cctccggatc ccgagccagg
     1381 ctgtaccagt aatcacgctg catgcccgat tcatcggccg caatcagcac acagctgacg
     1441 ctgtgtctgg ttccgaggta gctgccgatg aacccgtgac tgttgccgta gacccgacac
     1501 gactgctgcg tgctcacctg cgccccgtcg gagttcacga tgcgctcgtc gcggctgagc
     1561 ccggccgcct cgcaggcgat cgccagccgc tcggcctcgc cgacatccaa gcgccagggg
     1621 tgatacagat ccagctccgg cagcgcatcc ggcatcaaac cggcgtccgc caggccgttg
     1681 caggggtctt cctcggtgtg gcgggcaatg tcccgggccg cgcgcacggt gtcctcgatg
     1741 gccgtcaggc tgctgtccga cgtgctcgcc gagcccttgc gcaaaccgta gtagacggtg
     1801 attccaaatc cccggtcctg gttgaactcg acggtttcca gctcaccttt gcgcaccgaa
     1861 acggaaaggc cggcgtcctc gttcacggac acctcggcgg cgtccgcgcc ctggcgacgg
     1921 gcttcgtcca ggatgtcgcc gaccagggac gaaagccccg cctcgctgtc ttgcaggtca
     1981 atcaactcgt tcggcatgga gtcctcacat ttgcctctgc ttcgggtacg ctcgaccgat
     2041 gaccgagcct cccatcagca aatccgaacg caaacgcgcc gcagcgcgcc tgcaggagat
     2101 cggccgggcc ctggccgagc tgcgtcccgc caggctggac gagatcccct tgccggaaac
     2161 gctcgagcgc gccatctacg actaccggcg atttgcctcc cgggaggcca aaagacgcca
     2221 gctccagcgc atcggcaagc tcatgcgtga gatcgacacc gccccgatcg aggcggccct
     2281 ggagcgtctc gaggggcgct cggccagcgc caggtacgag cagcaccagc tggaacggtg
     2341 gcgcgaccgg ctgatcgagg atcccgccgc gctcaccgac tacctcgccg aacatccgca
     2401 ctccgagcgc cagacgctgc ggcacctgat cgagcgggcc cgcacggcag gcaacgagca
     2461 gcagcagaag acggcatccc gagccctgtt ccgctttctg cgggaagacg tgcccaccgg
     2521 ataggcacgc tcggcgggcg acgcgcgcgc ggccccaatc gccattgcgt tcacgcgctc
     2581 gttccgccga cggtgacctc gtcgatcttc aacgtgggct ggcccacgcc taccggaacc
     2641 gactgcccgt ccttgccgca aacgccgatg ccgcgatcga gctcgaggtc gttaccaacc
     2701 atggatatcc tggtcatgac ctcgggcccg ttgccgatga gggtggcacc acggacagga
     2761 tgggtcacgc gacccttctc gatgagatag gcctcggtgg ctgagaaaac gaacctgccc
     2821 gaggtgatgt ccacctgacc gccgccaaag ttgacggcat agatgccgcg gtcgacgctc
     2881 gcgatgatct cctcgggatc gtgctccccg gccttcatga aggtattggt catccgcggc
     2941 atgggcaggt gcgcaaaaga ctggcggcgc ccgttacccg tgctcgatgt cttcatcagc
     3001 ctggcattga gcttgtcctg catgtagccc cgcagcacgc cgttctcgat gagcaccgtt
     3061 tcctggctgg gcgagccctc gtcgtctacg gtcagcgacc cacggcgccc gtcgagggtt
     3121 ccgtcgtcca ccaccgtgca cagctcgctc gcgactttct cgccaacgcg gttggcaaac
     3181 gccgagctgc ccttgcggtt gaagtcccct tccagaccgt gccccaccgc ctcgtgcagc
     3241 agcaccccgg gccagccgtg gccgagcacg acctgcatgg gtcctgccgg cgcgtcgacg
     3301 gcttccagat tcaccagcgc cgtacgcacc gcctcccggg ccaggccgtc cgtgaattca
     3361 gaatcgctga ccaggccgag gtccccgcgg cctccggctc cggcggagcc ccgctcccga
     3421 cggccgtcct gctcgacgat caccgagacg tccaagcgca ccagcggcct gacatcggca
     3481 cagagcgtgc cgtcggaggc catcaccagg atcgactcat ggctcgccga gagacgaaca
     3541 ttcacctctt tgacgcgcgg gtctgcctgc cgggccgcct cgtcgacgcg ccgcagcagc
     3601 gcgaccttct cggagccatc cagcgtcgag atgggatcga cgctgggata gcgggcgggc
     3661 acgcgcggta cctgccaggc gggcacgctc cggtcttctc cgtgccgggc aatggatcgc
     3721 gcagcttccg ttgcgctcgt cagcgcgggc agaatgatgt cttcggcgta agcaaaaccc
     3781 gtcttctcgc cgctgatggc gcgcacgccc acgccacggt cgacgctgaa actcccgtct
     3841 ttgacgatac cgtcttccag cgcccaggac tcggagcgct gatgctgaaa gaacagctcg
     3901 cccacatcga tggagcggct gctgagccgg cccatcaacc tgtcgaggtc tgactgttcc
     3961 aggcccgccg gcgcccagag ctggccttga gcctcttcga gcagatcagt cacgacgccg
     4021 cattactcct gtggttccag ttccgtgaaa gtgtcctcgt cgccggcgcc tgccagcgac
     4081 tccagttcgg cctcgaccat ctcgctttcg aaaacgctga cgagattgac ctggggctcg
     4141 tccagcgtac caccgatctc gtacttggca ctgctgaact gctccagggg tttgcgcagc
     4201 accctttcgc caaccagtat gcccgctccg gccagcgggt tggcgagagc gatgtaggcc
     4261 gcgtaccacg gcaggctctt gctgaccgga agcgtcacga tcatctcgtt gtccagctct
     4321 ccggtgacga gatccacgtt ccccgcaacc tcgaactcgg atccagagcc cttcaccttg
     4381 aggggctcga cgaatgccag gtggccgcga tcgaaactga cggtggcctt gagcttgtca
     4441 aagctcaggc ccttgccggt gaggtccgag aagtcgccgc gcatgcgctt ggcgatggtg
     4501 tcgaagttga tgaggctgaa catgcgcacc gcgccgcccc ccgactcgac gtcgacgaag
     4561 ctgccggtct cggcccgaac cgaggcggtt ccgaccagca ggtcgagatc cacgttggcc
     4621 ggcgagccgg cccagctgag ctcgcccgtc atcccggcgg attcggtttc catgctgggc
     4681 gcaaagcccc actggggcag cacgtcgaag aggttctcgg cagcaagctc gcccttgaac
     4741 cgggactcgc cgtcgaccgt ggaccagtac acgccctccg gagaggacac cgtgacgccc
     4801 cgcacgacgg cctcgagatc ggcaagccgg acgccgtccg gttcggggtg aacgttgaat
     4861 ttccagcggc cgtaatccgc ttcgcccacc cggaactcgc cgacttcgac caccgcctcc
     4921 ggaagctccg gggccatctg cgggctgagc ggatccccct cgccgtcacc ggcgggcagg
     4981 accacccggt ctaagccaag cacgagcggc gcgtcgccgt ccatcgacac gcgaccgacc
     5041 acggcttcgc tgtcgatcgt gatggcgaaa ccttccgcgg tgatgtcgcc gtcgagcacg
     5101 gcatcgaaga tgacgaactc gtcgagcctg atctggccca cccgaaggcc gctcagcttg
     5161 agcggcatga aacccgatcc gccaacctcc tcgtcaggga ccacttcttc gagcgcgaaa
     5221 ccctcgagat ccccggtgag cagcagatag tcggctcggc catccagaac cggcggcggc
     5281 tgaccaaagc ccaccgcgcc gcgctcaggc gcgtccgcga cgtgcagcca accctgggcg
     5341 gtaccgtggc ggaagctgat gatctgccgc tcgtccagaa actgcacggc aacttcaacc
     5401 ggtcgggcga cctccgcggg cttgcgcaga tcggccggaa gatccagcgc catgccctga
     5461 agatcggata cgacctccag ccgggtgacg tgatccggat ccacgcccac gtgcagcgag
     5521 gccaggaagt cggcgctgcc ggtcaccacg ccaggatctt ccatgtcgag gacggccagc
     5581 acgtcctcgt gggtggctcg cccagcgaag ctgaggatca tctcctgcga attgctgtcg
     5641 gcatcgatga gaacgtcgtt gccgaacagg gtaccgctga tccgggagga cctgagcccg
     5701 ttcggatacc ggtaccgcag atctccctcc agatccccca gggccagacg gaaatcgggc
     5761 atccggagcc cggcgtccaa cattctgacg ttgacgtcca cctgcagctg ctcggaggcg
     5821 tccacgtcct cagggagctt gatgggaatg tgcaggttgc cgagcacctc gagccggcca
     5881 tccccgaccc actccggcgc cacaaatccc atggactccg caaggggcgt ggttcgcacg
     5941 aagtccagcg ccaggtcggt ctccattcga gatacgagct ccacgtccgc gtaggaggcg
     6001 ttgttgcgca gcacgacctg ggagccgccc agccggacgc cttcgcttgc gcccttgtcg
     6061 acggtgacac gcaccatcgg accggcgacc acgacgtcgg cgtcgagctc gtggacgtcc
     6121 ggccagtctg gcgcgtaggc aatgcggcca tcgctgatgc gggctgacag ctcgatccgg
     6181 cgggccagct cgttcacctt gaggtgctgc tggccgtgat agccgaaccg aatgcgcgtc
     6241 agcaggccgg ctttcagccc gccacgcagc cagtcctcga gaccctcgtc gatggtgtac
     6301 ggcacgaacg tgaacgcctg atcggtgtcc agagcgtcca cgccgatgag aagagaaagc
     6361 cgctcctcgt agcgctcgcc gggcggccgc gtgagggcaa agctgcccgc cgccctgctg
     6421 aacagggtct cggcccgcag gttgacgcct cgaaggccga gataaccgtt gccgaaccag
     6481 gcctgaaacg ctccctgcag gtagggcatc tgccaggtat ccgtgaaggt ctggggaaac
     6541 ttgaggagca cgtcctcgct cttcagcttc agctccagcc cccgggcgtg gcccaaaagc
     6601 tctcccgccg ccccgcgaac gaacggggca ccgcggtagc cgtcaacgtc caggccggcc
     6661 agcgtggcgg caaacccgct ctctcccgtg ggcagtcgat agtaacctcg gacgttgagc
     6721 gcttcggcct gcagcttgag agcgctcaac cagcggcgcg cgcgctccga acccctgagc
     6781 gctcgctcga gaaactcgga tacgccaccc atgtccaccc gttgagccca gaactgaacc
     6841 acttccgcat ctttgcggac gtgcacggcg ggcatgcgca atgcccgctg cgtgcgcacc
     6901 tgctcctcgg aatcgtccag atgggcaacc agcccaaagg cc
//
LOCUS       DBNHOX010000632         3652 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_693470, whole genome shotgun sequence.
ACCESSION   DBNHOX010000632 DBNHOX010000000
VERSION     DBNHOX010000632.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_693470"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="AC16UZ_13915"
     CDS             complement(<1..1056)
                     /locus_tag="AC16UZ_13915"
                     /inference="COORDINATES: protein motif:HMM:NF015662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR6247829.1"
                     /translation="MPPVLALKDSSSERRSFSARAAVLFALATCLVSVLILRLLQLQV
                     WEYDTYRTRSEENRIQVQPLAPPRGLIFDRNGVLLADNRPVHSLALVRERIADLDALI
                     TELGSLVSISEAEIESLRGRLLRRRRPYEPVALKLTLSEEDIAVLAVNRHRLVGVEIS
                     TQLVRYYPFGPLLAHAVGSVRRVTEDDLRELDPVRYSATKFVGRRGVERFYERSLHGE
                     VGYQRVETDAHGRIRQVLEINEPAAGQNITLQLDSRLQIAASAALGDRRGAIVALDPR
                     SGGVLALVSHPQYDPNQFVTGMTSAQFRELTSSRDTPLFNRAINGQYAPGSTFKPIVG
                     LAGVASGVTRWADQIDDQ"
     gene            complement(1058..1525)
                     /gene="rlmH"
                     /locus_tag="AC16UZ_13920"
     CDS             complement(1058..1525)
                     /gene="rlmH"
                     /locus_tag="AC16UZ_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR6247830.1"
                     /translation="MKIRVIAVGARPRPWVADGFREYARRLPREMPLSLVEVAPGGGR
                     GRSAEQVVAMESERLLSHLGPRDWVVALDVGGEGCSTRELALSLESWRMQGRDVAFLI
                     GGAEGLAPTCLARADMALSLSELTFPHEMVRVILAEQIYRAWTILSGHPYHRD"
     gene            complement(1545..1892)
                     /gene="rsfS"
                     /locus_tag="AC16UZ_13925"
     CDS             complement(1545..1892)
                     /gene="rsfS"
                     /locus_tag="AC16UZ_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR6247831.1"
                     /translation="MTAEALRDLVVDALEERKGRDVVAMDVTKSTDITDFMIIASGTS
                     NRHVKALVDQVVESAKAGGNPPLGVEGRETHEWVLVDLGDVLVHVMQADTRAFYDLER
                     LWSDIPLDSETLN"
     gene            complement(1882..2514)
                     /gene="nadD"
                     /locus_tag="AC16UZ_13930"
     CDS             complement(1882..2514)
                     /gene="nadD"
                     /locus_tag="AC16UZ_13930"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR6247832.1"
                     /translation="MIALFGGTFDPVHRGHLHGAATVCEALGLEAISMVLSARPGHRA
                     SPGASAEHRWEMLCLACADDGRLVPDDRELHRDRPSYTVETLLEVRGEIGDESLVWVL
                     GSDAYADLTSWHRWGEVLDLTNLVVLERPGGPSRWAPPLDGISRRHRVEGMLDAPWGQ
                     ILFLKAPMQEVSASEIRAAIAGGRPVAHLLPERVATYISRHGLYGAPSDS"
     gene            complement(2511..>3652)
                     /locus_tag="AC16UZ_13935"
     CDS             complement(2511..>3652)
                     /locus_tag="AC16UZ_13935"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR6247833.1"
                     /translation="DEDRETLKRANEVDVRAAGDKGVDQPLIDRLVLDDRAIDRMLEG
                     VLQVDKLADPVGEITDLAYRPTGIQVGRMRVPLGVIGIIYESRPNVTADAASLCLKSG
                     NACILRGGSEAIESNRAIAAGIARGLAAAGLPETVVQLVDTTDRDAVGELLKADRFVD
                     VIVPRGGKGLIERIIRESRIPVIKHLDGVCHVYIDDAADLEMAAAIAVNSKIEKYAVC
                     NAMETLLVANGVADRVLPVLGDRFRQAGVELRGCERARAAFSDMKPATEEDWREEYLG
                     PVLAVRVVEGIDEAIAHINTYGSQHTDAIVSNDYGRTRRFLAEVDSASVMVNASTQFA
                     DGFEFGLGAEIGISTDKLHARGPVGLEGLTSQKFVVFGGGEIRHR"
BASE COUNT          651 a         1260 c         1128 g          613 t
ORIGIN      
        1 ctgatcgtcg atctgatccg cccacctcgt cacgccgctc gcgacccccg cgagccccac
       61 aatgggtttg aaggtcgagc ccggcgcgta ctggccgttg atcgcacgat tgaacagcgg
      121 ggtgtcccga gaggacgtca gctcccgaaa ctgggccgac gtcatgccgg tcacgaactg
      181 gttggggtcg tactgcgggt gactcaccag ggccagcacg ccaccgctgc gggggtccaa
      241 ggccacgatg gcgccgcgcc gatctcccag ggcagccgac gcggcaatct gcaggcggct
      301 gtccagctga agcgtgatgt tctgaccggc cgcaggctca ttgatctcca gcacctggcg
      361 gatccggccg tgggcatcgg tctccaccct ctgatagccc acctcgccgt gcagcgagcg
      421 ttcgtaaaag cgctccaccc ctctgcgccc gacaaattta gtggcgctgt agcgaacggg
      481 gtctagctcg cgcagatcgt cctcggtgac tcgtcgaacc gagcccacag cgtgcgcaag
      541 aagcggacca aaggggtagt agcggacgag ttgggtggag atctcaacgc cgacgagccg
      601 gtggcggttt acggcaagca cggcgatgtc ttcctcgctg agcgtcagct tgagcgccac
      661 cggctcgtag ggccgtcggc gtcgcagcag gcggccgcgg aggctttcga tctccgcctc
      721 gctgatgctg accagcgagc ccagctcggt gatgagggca tcgaggtcgg ctattcgttc
      781 gcgcaccaga gcaaggctgt gaacgggacg attgtcggcc agcagcacgc cgttgcgatc
      841 aaaaattagc cctcgtggag gcgccagcgg ctgaacctgt atgcgatttt cttccgaccg
      901 ggtccggtac gtgtcgtact cccacacctg cagctgcagc agacggagta tcaggacgga
      961 tacgaggcag gtagcgagcg cgaacagcac cgcggcccgg gcagagaagg accggcgttc
     1021 ggacgagctg tctttgagtg cgagaacagg cggcatgtca gtcccgatga taggggtggc
     1081 cggagagaat ggtccaggcc cggtagatct gctccgccag gattaccctc accatctcgt
     1141 gggggaaagt cagctcggac agcgacagcg ccatgtcagc acgagccagg cacgtgggcg
     1201 cgagcccttc ggcgccccca atgaggaaag cgacgtcccg cccctgcatc cgccagctct
     1261 ccaggctcag cgccagttcc cgggtgctgc aaccctcacc tcctacatcc agggcgacga
     1321 cccagtctct tggacccagg tggctgagta atcgttccga ttccatcgcc acgacctgct
     1381 cggcagaccg gccacggccc ccgccgggcg ccacctccac aagagacagc ggcatttccc
     1441 gcggcaaccg cctggcgtac tcgcgaaacc cgtcggcaac ccagggccga ggtcgtgcgc
     1501 ccacggcgat gacccggatc ttcatggcag ccttcccggg cgcctcagtt gagcgtttcg
     1561 gagtccaacg gtatgtcgga ccagagccgc tcgaggtcgt agaatgcccg agtgtcggcc
     1621 tgcatcacgt gcacgagcac gtcaccgaga tccaccagca cccactcgtg ggtttcacgg
     1681 ccctccacgc cgagcggcgg gttaccgccg gctttcgccg actccaccac ctgatcgaca
     1741 agtgccttca catgccgatt ggaggtgccg ctggcgatga tcatgaaatc cgtgatgtcg
     1801 gtactcttgg tcacgtccat cgccacgacg tcgcggcctt tccgttcttc caacgcatcc
     1861 acgaccagat cgcgcagggc ctcagctgtc actcggtgct ccgtatagtc cgtgccgact
     1921 aatatatgtt gccactcgct ccgggagcaa atgcgccacg gggcgcccgc cggcgatggc
     1981 tgcgcgaatt tccgaggcgg acacctcctg catgggagct ttcaggaaga gaatctgccc
     2041 ccacggcgcg tctagcatcc cctcgacccg gtgacgacgg ctgattccat ccaggggcgg
     2101 ggcccagcgg ctcggcccgc ccggccgctc cagcaccacg aggttggtca gatccagaac
     2161 ttcaccccag cgatgccagc tggtcagatc tgcgtaggcg tcgctgccga gcacccagac
     2221 cagggactcg tcaccgattt ctccgcggac ctccagcagc gtctccaccg tgtaggacgg
     2281 ccggtcacga tgaagctcgc gatcatccgg aaccaggcgc ccatcgtcgg cgcacgccag
     2341 gcacaacatc tcccagcggt gctcggcgga cgctcccggc gaagcgcggt gacccggccg
     2401 ggcggacagc accatggaga tggcttcgag gccgagcgcc tcgcagaccg tggccgcgcc
     2461 gtgcagatgt ccccggtgaa ccggatcgaa ggtgccgcca aacagagcaa tcaacggtgc
     2521 cggatctctc cgccgccgaa gaccacgaac ttctgcgacg tgaggccttc gaggcccacc
     2581 gggccacggg catggagctt gtccgtcgag atgccgatct cggctcccag gccgaattcg
     2641 aagccatctg caaactgggt cgaggcgttg accatgaccg acgccgagtc cacctccgca
     2701 agaaatcgac gggttctgcc atagtcgttg gagacgatgg cgtccgtatg ctgggacccg
     2761 taggtgttga tgtgagcgat cgcctcgtcg atcccctcca ccaccctcac cgccagcacc
     2821 ggcccgagat actcctcccg ccagtcttcc tcggttgccg gtttcatgtc cgaaaaggcg
     2881 gcccgagccc gctcgcagcc tcgcaattcc acgcccgcct ggcgaaaacg gtcgccgagc
     2941 acgggcagga cccgatcggc tacgccgttg gccaccagca gcgtctccat ggcgttgcag
     3001 accgcgtact tctcgatctt ggagttgacg gcgatggcgg ccgccatctc caggtcagcg
     3061 gcgtcgtcga tgtagacgtg gcacacgccg tcgaggtgct tgatgaccgg gattcgtgat
     3121 tcccggatga tgcgctcgat caggcccttg cctccccgag gcacgatgac gtccacgaac
     3181 cgatccgcct tgagcagctc tcccaccgca tcgcgatccg tcgtgtcgac gagctggacg
     3241 accgtctccg gcagtcctgc cgcggccagg ccacgggcaa ttcccgcggc gatggctcgg
     3301 ttcgactcga tggcttccga accgccccga aggatgcagg cgttgcccga cttcaaacac
     3361 aggctggcag cgtccgcggt cacgttcggg cgcgattcgt agatgatccc gatcacaccc
     3421 aggggcacgc gcatccgccc cacctgaatg cccgtcggac ggtaggccag atcggtgatc
     3481 tcccccaccg gatcggccag cttgtccacc tggaggacgc cttccagcat tcggtcgatg
     3541 gcacgatcgt cgagaaccag acgatcgatg aggggctgat cgacgccctt gtctcccgcg
     3601 gcgcgcacgt cgacctcgtt agcccgcttc agcgtttccc gatcttcatc ga
//
LOCUS       DBNHOX010000633        12142 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1271510, whole genome shotgun sequence.
ACCESSION   DBNHOX010000633 DBNHOX010000000
VERSION     DBNHOX010000633.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12142)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12142
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1271510"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="AC16UZ_13940"
     CDS             <1..513
                     /locus_tag="AC16UZ_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR6247834.1"
                     /translation="DLTAAEVELLSQPQREHRMRRRLAEDAARFDFVIIDCPPSLNIL
                     TLNALIAADGVLIPMQCEYYALEGLTALLETVEGVRQQANPSLSIEGLLRTMYDPRNA
                     LTRDVSRQLIQYFGDRVFRTVIPRNVRLAEAPSHGLPAILYDRQSRGAVAYMALAGEI
                     KRKHEEDDDP"
     gene            518..1402
                     /locus_tag="AC16UZ_13945"
     CDS             518..1402
                     /locus_tag="AC16UZ_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009402535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR6247835.1"
                     /translation="MRVTKKRLGRGLDALLSGEVVADLGAGQKGPGIPEGVQVAEIGL
                     DQVVPNRYQPRRDFNEEALAELVASIRSQGVMQPVVVRRRAQGGYELVAGERRWRAAG
                     LAGLDAIPALIRDVSDEQASAMALIENIQREDLNPLEEAHALQRLRDEFGLTQQQVAD
                     AVGKSRVAVTNLLRLLNLGPAVRDLLVAGAIEMGHARALLSLDSVVQERAANDVVARG
                     LSVRQTEALVRRLQQGKGPGRPSEAVRDADTAHLERELSEKIGAPVSISCNAKGRGRL
                     VIRYGSLEELDGILGHIR"
     gene            1548..1886
                     /locus_tag="AC16UZ_13950"
     CDS             1548..1886
                     /locus_tag="AC16UZ_13950"
                     /inference="COORDINATES: protein motif:HMM:NF015834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HIR6247836.1"
                     /translation="MPYRTVALQALAGLGVVLAALAWDAREAVAALAATIVSVVPNAY
                     FAWRVSRERAAARILAAEVVKVVATVGMMGLAFATMELRPLGFFATFGVLQLAHVVGG
                     TSLAGKATSR"
     gene            1969..2817
                     /gene="atpB"
                     /locus_tag="AC16UZ_13955"
     CDS             1969..2817
                     /gene="atpB"
                     /locus_tag="AC16UZ_13955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR6247837.1"
                     /translation="MSSGTVTSGEYIQHHLTNLTFGRHPDGTWGFAQSAQEAAAMGFN
                     AINVDSMGWSIGLGLVFVAVFYTAARKATSGVPGGLQNFVEIIIEFIDEITHSIFTHK
                     NDMIAPMALTVFVWVFLMNLMDLIPVDWIPGLAAAMGISHMKIVPTTDVNITMAMAGS
                     VFGLIIYYSIKCKGVGGFLGELAFHPLPKAAAPANLFLEGVTLIAKPLSLGLRLFGNM
                     YAGEMIFILIALLFPAGIVYGLLGGVLQWGWAVFHVLIITLQAFIFAVLTVVYLAQAH
                     DVMEEH"
     gene            2875..3111
                     /gene="atpE"
                     /locus_tag="AC16UZ_13960"
     CDS             2875..3111
                     /gene="atpE"
                     /locus_tag="AC16UZ_13960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HIR6247838.1"
                     /translation="MELEVLLYVAGGLMMGLGAIGAAIGVGVLGGKFLEGVARQPELL
                     PMLRTQLFIVLALVDAVPMIAVGIGLYTLFAVAG"
     gene            3181..3651
                     /locus_tag="AC16UZ_13965"
     CDS             3181..3651
                     /locus_tag="AC16UZ_13965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019694394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR6247839.1"
                     /translation="MNINWTIVGQSLTFFVFVWFCWKFIWPPIVKAMRERQEAIAEGL
                     ASSERAAKDLELAQERATDQLREAKDEAHKIIEQARGRANQMIDEAKNEAREEVERIR
                     EGARAEIDQEINRAKEALRGQVAALAVSGAERVLGSAIDAERHRELLDQLATDL"
     gene            3663..4202
                     /locus_tag="AC16UZ_13970"
     CDS             3663..4202
                     /locus_tag="AC16UZ_13970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIR6247840.1"
                     /translation="MAELATLARPYANAVFATARDEGNLERWSRMLGFLAAAVREPEV
                     RRLIGSPDVSDEQKAFRLADVCGDELNDKARKLVQVLAGNKRLGLIDEILEQFEHKRA
                     QDEQTLDVEVVTAYPLSDDQAARLKQALGEKYAKEVNLSSVVNEDLIGGAIIRAGDTV
                     IDGSVRGRLIKLAESLQRI"
     gene            4267..5811
                     /gene="atpA"
                     /locus_tag="AC16UZ_13975"
     CDS             4267..5811
                     /gene="atpA"
                     /locus_tag="AC16UZ_13975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR6247841.1"
                     /translation="MQQLNPSEISDIIRQRIEGLDVRAQAQNEGTIVGVSDGIVRIHG
                     LADAQYGEMIEFDGGLYGMALNLERDSVGVVILGDYEGLSEGMTARCTGRILEVPVGR
                     ELLGRVVDSLGNPIDGKGPLNASETAPIEKVAPGVIARQSVDQPVQIGLKCIDSMVPI
                     GRGQRELIIGDRQIGKTAIAIDAIINQKDSGIRCVYVAIGQKMSTIANIVRTLEEHGA
                     MENTIVVAASASDPAPMQFIAPYSGCSMGEYFRDNGEDALIIYDDLTKQAWAYRQISL
                     LLRRPPGREAYPGDVFYLHSRLLERAARVNADYVEQYTGGKVKGRTGSLTALPIIETQ
                     AGDVSAFVPTNVISITDGQIFLETDNFNSGLRPAMSPGISVSRVGGSAQVPFIKKIAG
                     GIKLALAQYRELAAFSQFASDLDDATRRQLEHGRRVTELMKQKQYAPMSVAEMGVSLY
                     AANEGYLVDIAVDQVLEFEQGLLAYMNSEKADFMRRINETGDYDDGIVAEMRQGIEAY
                     KAQANF"
     gene            5822..6682
                     /gene="atpG"
                     /locus_tag="AC16UZ_13980"
     CDS             5822..6682
                     /gene="atpG"
                     /locus_tag="AC16UZ_13980"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR6247842.1"
                     /translation="MAVGKEIKKKIGSVENTKKITSAMQMVAASKMRRTQERMREGKP
                     YSEKIRAVIGHLANSNPEYRHVYMEPREISRIGYLVVSTDKGLCGGLNINLFRTLIRD
                     MAEWHAKGKEIDLGLIGNKAAAFFRSVGGNIVAAMNNVGEDPALADLIGGIKVMLDAY
                     EEGKIDRLFIASNEFVNTMTQRPVVRQLLPLDPETDDSMRHRWDYIYEPDARQLIEGL
                     VTRFMESQVYQAVVENGACEQAAKMVAMKSATENAEKLIDELTLIYNNARQAAITQEI
                     AEIVGGAAAV"
     gene            6714..8096
                     /gene="atpD"
                     /locus_tag="AC16UZ_13985"
     CDS             6714..8096
                     /gene="atpD"
                     /locus_tag="AC16UZ_13985"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR6247843.1"
                     /translation="MSSGRIVQIIGAVIDVEFSREDVPKVYDALKVSSTGLTLEVQQQ
                     LGDGVVRAIAMGPSDGLSRGLEVTNTQQPISIPVGEETLGRIMNVLGEPIDEKGPVNA
                     QQKMPIHRKPPSYEDQKGGNEILETGVKVIDLICPFAKGGKVGLFGGAGVGKTVTMLE
                     LIRNIATEHSGLSVFAGVGERTREGNDFYHEMEESGVLDKISLVFGQMNEPPGNRLRV
                     ALTGLTLAEQFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPNLAEEMGV
                     LQERITTTKTGSITSVQAVYVPADDLTDPSPATTFAHLDSTVTLSRAITDLGIYPAVD
                     PLDSTSRQLDPLVVGEEHYQVARGVQQVLQRYQELRDIIAILGMDELSEEDKQLVYRA
                     RKMQQFFSQPMFVAEPFTGQPGVYVSREDTVRSFKGILEGEYDHLPEQAFYMVGTIEA
                     AIEKAETLSA"
     gene            8108..8530
                     /locus_tag="AC16UZ_13990"
     CDS             8108..8530
                     /locus_tag="AC16UZ_13990"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014873131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HIR6247844.1"
                     /translation="MAMTFHCDIVSAEREIFSGRVTMLSAIGTIGELGILPGHAPLLT
                     GIRPGPVRLRMDNGEEEVFFASGGYLEVQPGVVTVLADTALRAEDIDEAAAAAAQQDA
                     ERALREQTADIEFSAAAAMLAEATARQRTLAELRKRRR"
     gene            8701..10092
                     /gene="glmU"
                     /locus_tag="AC16UZ_13995"
     CDS             8701..10092
                     /gene="glmU"
                     /locus_tag="AC16UZ_13995"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR6247845.1"
                     /translation="MSSSPRLEVIVLAAGQGKRMNSRLPKVLHPLAGRPLLAHVLETV
                     DELEPAGIHVVIGHGADLVREAIGAAALRWVVQTEQLGTGHAVQQALPDIDDSAVVLV
                     LCGDVPMVTAQTLRRCVTGAGNGAMAVITAELSDPAQLGRILRGTDGAVRGIVEYKDA
                     SAAEKAISEINSGIIAAPAGILRDLLAEVRPDNAQGEYYLTDIVALAVSRGIEVLGLK
                     AERPEEVLGVNDRNQLAVLERRYQLERAGELMAAGATLADPSRLDVRGAVTVGADCFI
                     DVNVVFEGTVVMGREVSIGPGSVIRDSQLGDGVRVEAHTLVDGARVEAGCSLGPFARI
                     RPGTQLGEGVKIGNFVETKKAVLGAGSKASHLTYLGDAILGANCNVGAGTVTCNYDGV
                     DKHRTTIGDGVFVGTNSTLVAPVTLGDDAFVAAGSTITGDVEKGELAVGRGRQRNVQG
                     WVRPGRRDRADED"
     gene            10108..11940
                     /gene="glmS"
                     /locus_tag="AC16UZ_14000"
     CDS             10108..11940
                     /gene="glmS"
                     /locus_tag="AC16UZ_14000"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR6247846.1"
                     /translation="MCGIVGAVADRNVAPILLEGLQRLEYRGYDSAGLAVIDGAGEMD
                     CRRRVGKVKELAGDLSARRCDGHMGIAHTRWATHGVPSENNAHPHVSNGRIALVHNGI
                     IENFEELREELQSEGYAFASETDSEVIVHLVDSYFDKGLSLLEAVRSAVKRLEGAYAI
                     GVIALADPDRIVAARMGSPLVVGKGLGENFIASDPLALRPVTDRFVFLEEGDLVEITR
                     DEISIWNVENESVIRASVRVELGHDDVDRGNYRHFMQKEIYQQPEVLAATLEGRIGRT
                     RVLEPAFGVQAGQIFDKVQAVTIVACGTSYYAGSIARYWIEELARIPCQVEIASEFRY
                     RQVAVTPGNLFVTISQSGETADTLAALRVAKELGYVSTLTICNVATSTLVRESDIALM
                     TQAGTEVGVASTKAFTTQLADLLLLTILIARRHGMDAAREQELVEALAGLPNAVEQVL
                     GLDGRIRVLAERFMDRSNALFLGRGPMFPVAMEGALKLKEISYIHAEGYPAGELKHGP
                     LALVDENMPVVAVAPNDELLEKVQSNLQEVRARGGALYVFADERTGFKEEPGVTVIPL
                     PPVHDTLAPIVYVVPLQLLAYHVAVLKGTDVDQPRNLAKSVTVE"
     gene            12015..>12142
                     /locus_tag="AC16UZ_14005"
     CDS             12015..>12142
                     /locus_tag="AC16UZ_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR6247847.1"
                     /translation="MDVTNILEHLNKDQREAVSAPLGNALVVAGAGSGKTRVLVHR"
BASE COUNT         2239 a         3710 c         4083 g         2110 t
ORIGIN      
        1 gatctcactg ccgccgaggt ggaactgctg agccagccgc agcgcgagca caggatgcgg
       61 cggcggttgg ccgaggatgc ggcgcgcttc gacttcgtca tcatcgactg cccaccatcg
      121 ctgaacatac tgaccctcaa cgctctgatc gccgctgacg gcgtgctcat tcccatgcag
      181 tgcgagtact acgccctcga gggcctgacg gctctgctcg aaacggtcga aggcgtccgg
      241 cagcaggcca acccctcgct gtccatagag ggcctgttgc gaaccatgta tgatccccgc
      301 aacgccctca cgcgggacgt ttcccggcag ctgatccagt atttcggcga ccgggtgttc
      361 cgaacagtga tcccgcggaa cgtccggctg gcagaggcgc ccagtcacgg gcttccagcc
      421 attctctacg atcggcagtc gcggggcgcg gtggcctaca tggcgcttgc gggggaaatc
      481 aagcgcaagc acgaagagga cgacgacccg tgagcaattg cgcgtgacca agaagagact
      541 gggcagaggt ctggacgcgc tgttgtcggg cgaggttgtg gccgatctgg gcgcggggca
      601 gaagggccct ggaatccccg aaggcgttca ggtcgccgaa atcgggctcg accaggtggt
      661 accgaaccgc taccagcccc gccgggattt caacgaggaa gcccttgccg agctggtcgc
      721 ctccatcaga tcccagggag tgatgcagcc cgttgtggtc agacgacgag cccagggcgg
      781 atacgagctc gtcgccggtg agcggcggtg gcgtgctgcc ggccttgcgg gcctggacgc
      841 cattccggcc ctgattcgtg atgttagcga tgagcaggct tcggccatgg cgctcatcga
      901 gaacatccag cgcgaggatc tcaacccgct ggaagaggcc cacgccctgc agcggctgcg
      961 agacgagttc ggcctcaccc agcagcaggt cgccgatgcg gtaggcaagt cgcgcgttgc
     1021 ggtgaccaac ctgctgcgcc tgctgaatct ggggcctgcg gtgcgggacc tgctggtggc
     1081 aggagcgatc gagatgggcc atgcgcgcgc cctgttgtcg ctggacagcg ttgttcagga
     1141 gcgcgcggcc aacgacgtgg tcgcccgggg gctttcggtt cgccagaccg aggcgctggt
     1201 gaggcgcctg cagcagggca aggggcccgg gcgcccgtcc gaggccgtcc gcgatgcaga
     1261 caccgcgcac ctggaacgcg aactctctga gaagatcggc gcgccggttt cgatctcctg
     1321 caatgccaag ggccgaggtc gcctcgtcat tcggtacgga tcgctcgagg agctcgacgg
     1381 tatactcggt cacatcaggt aggcgccggc gccgctcaga tgcgcgtcgt tcgaaatggc
     1441 cgctttgtca ggatcacgct aattgaacgc gctctggccc gtctctataa tacgcgccgc
     1501 ctttcggggg ttggtgtttg tcaggctgtc ctgacgtgga catgtctgtg ccgtatcgaa
     1561 ccgtggcgct gcaggcgctc gccgggctcg gggtggtgct ggcagcgctg gcgtgggacg
     1621 cccgtgaagc ggtggcggcg ctggccgcta cgatcgtcag cgtcgtgcca aacgcttact
     1681 tcgcctggcg cgtcagccgc gagcgggcgg cagccaggat cctcgcggca gaggtcgtga
     1741 aggtggtcgc caccgtgggc atgatgggat tggcctttgc gacgatggag ttgcggcctc
     1801 tgggtttctt cgccaccttc ggcgtgctgc agctggcgca cgtggttggc ggaacgagcc
     1861 tggcgggaaa agcgacttca cgctgaacgc cggcgcgttt gttgacgcgc tggtcaaatt
     1921 gccggggacg tggtcttcgg cagacattgg ggacgagagc aacgcagcat gtcgagcgga
     1981 accgtaacct ccggggagta catccagcat cacctgacga acctgacgtt cggtcgccat
     2041 cctgacggca cctgggggtt tgcccaaagc gcccaggagg cggcggccat ggggttcaat
     2101 gccatcaacg tggattccat gggatggtcc atcgggctcg gcctggtttt cgtggccgtt
     2161 ttctacaccg cggcgcgcaa ggctacgtcc ggcgtcccgg gcggtctgca gaacttcgtc
     2221 gagatcatca tcgagttcat cgacgagata acccacagca tcttcaccca caagaacgac
     2281 atgatcgcgc ccatggcgct caccgtcttc gtctgggtgt ttctcatgaa cctgatggac
     2341 ctgatcccgg tggattggat cccggggctg gccgcagcca tggggatcag ccacatgaag
     2401 atcgttccga ccacggacgt gaacatcacc atggccatgg ccgggtcggt gttcggcctc
     2461 atcatctact acagcatcaa atgcaaaggt gtcgggggtt tcctcggcga gctggccttc
     2521 caccccttgc ccaaggcggc cgcgccggcg aacctgtttc tagagggggt gaccctgatt
     2581 gccaaaccgc tttctctggg cctgcgactg ttcggcaaca tgtatgcggg ggagatgatc
     2641 ttcatcctca tcgcgctgct gtttccggcg ggcatcgtct acgggctgct gggaggcgtg
     2701 ctgcagtggg gatgggcggt cttccacgtc ctcatcatca ccctgcaggc tttcatcttt
     2761 gcggtcctga cggtggttta cctggcacag gcccacgacg tgatggaaga gcactaagct
     2821 ctcctgatgg gaaattccga gagcggaaac aacaactcgt aactgggagt gatcatggaa
     2881 ctagaagttc tcttatatgt agccggtgga ctcatgatgg gtctgggcgc cattggggcc
     2941 gccatcggcg tgggtgttct cggcggcaag tttctggaag gcgtcgcgcg ccagcccgaa
     3001 ctgcttccga tgctccggac ccagctcttc atcgtgctgg cgctggttga cgccgtgccc
     3061 atgatcgcgg tgggtatcgg tctgtacacc ctctttgcgg tggcaggtta gccacccgac
     3121 ggaactgccg gccacggtgt gccgtgacgg tctgtcgaaa cgaatcgaag aggtgagttc
     3181 gtgaacatca actggaccat cgtcgggcag agcctgacgt ttttcgtttt cgtctggttc
     3241 tgctggaaat tcatctggcc gcccatcgtc aaagccatgc gcgagcgtca ggaagccatt
     3301 gccgaggggc tggcatcgtc cgagcgtgcg gccaaggacc tggagcttgc ccaggagcgc
     3361 gccacggatc agctgcgcga ggccaaggac gaagcccaca agatcatcga gcaggcccgg
     3421 ggccgggcca accagatgat cgacgaggcc aagaacgagg cgcgcgaaga ggtcgaacgc
     3481 atcagggaag gcgcccgggc ggagatcgat caggagatca accgcgcgaa agaagcgctc
     3541 cggggtcagg ttgccgcgct ggcggtttcc ggcgccgagc gggtgctggg ttccgccatc
     3601 gatgccgagc ggcaccgcga gctgctcgac cagcttgcca ccgacctgtg acggtctgac
     3661 caatggcaga actagcaacc ctcgcccgcc cttatgccaa cgcggtattt gccacggctc
     3721 gcgacgaagg caacctggag cgctggtccc ggatgctcgg ctttctcgcc gcggctgttc
     3781 gggagcctga ggttcgtcgg ctgatcggct caccggacgt gagcgacgag cagaaggcgt
     3841 tccggcttgc cgatgtttgc ggcgacgagc tgaacgacaa ggcgcgcaag ctcgtccagg
     3901 tgctggccgg gaacaagcgg ctcgggctca tcgacgagat actcgagcag ttcgagcaca
     3961 agcgcgccca ggacgagcag accctcgatg ttgaggtggt gaccgcctac cccttgagcg
     4021 acgatcaggc cgccaggctg aagcaggcgc tgggggaaaa gtacgccaag gaagtgaacc
     4081 tctccagcgt cgtgaacgaa gatctgattg gcggcgccat catcagggcc ggggataccg
     4141 tcatcgacgg ctccgtgcgc gggcgtttga tcaagctggc agagtcgctg cagcgtatct
     4201 gaatacagag gccttcggcg ggaacgacac cgtcgaggct ccaagaaaga gactgaggaa
     4261 ttcgaaatgc aacaactgaa tccttccgag atcagcgaca tcatcaggca gcggattgaa
     4321 ggattggacg tacgcgcgca ggcgcagaac gaaggcacca ttgtgggcgt gtcagatggc
     4381 atcgtccgca tccacggcct tgccgacgcc cagtatggcg agatgatcga gttcgacggg
     4441 ggcctctacg gaatggcgct caacctcgag cgcgactctg tcggcgtggt gatcctcggc
     4501 gactacgagg gtctctccga ggggatgacg gctcgatgca ccgggcggat tctcgaagtc
     4561 ccggtgggcc gcgagctgct cggccgggtg gtggattccc ttggcaaccc cattgacggc
     4621 aaagggccgc tgaacgcctc ggaaaccgcg cccatcgaaa aggttgcccc cggcgtgatt
     4681 gcgcggcagt ccgtggacca gcccgttcag atcgggctga agtgtatcga ctccatggtg
     4741 cccatcggcc ggggtcagcg cgagctcatc atcggcgaca ggcagatcgg caagacggcc
     4801 atcgccatcg acgccatcat caaccagaag gacagcggca ttcgttgtgt ctacgtggcc
     4861 atcgggcaga agatgtcgac gattgccaac atcgtccgga ccctcgaaga gcacggcgcc
     4921 atggagaaca ccatcgtcgt ggctgctagc gcatccgatc cggcgcccat gcagttcatc
     4981 gcgccttact ccggctgcag catgggcgaa tacttccggg ataacggtga agacgccctg
     5041 atcatttacg acgatctcac gaaacaggcc tgggcctacc ggcagatctc gctcctgctg
     5101 cgccggccgc cgggccgcga ggcttatccc ggcgacgtct tctatctgca ctcgcgtttg
     5161 ctcgagcgtg cggcaagggt gaacgccgat tacgtggagc agtacacggg gggtaaggtc
     5221 aagggccgga ccgggtccct gacggcgctg ccgatcatcg aaactcaggc gggggacgta
     5281 tccgcgttcg tgccgacgaa cgtcatctcc atcaccgatg ggcagatctt cttagagacg
     5341 gacaacttca actcggggct gcgccctgcc atgagcccgg gcatctcggt atcccgggtg
     5401 gggggatccg cccaggtgcc cttcataaag aagattgccg gtggcattaa gctcgccctt
     5461 gcccagtacc gggaactcgc agcgttctct cagtttgctt cagaccttga cgacgcgacg
     5521 cggcgccagc tcgagcacgg ccggcgagtc acggagctca tgaagcagaa gcagtacgcg
     5581 ccgatgagcg tagcggaaat gggcgtgagc ctgtatgcgg cgaacgaggg ctacctggtg
     5641 gacatcgcgg tcgatcaggt gctcgagttc gagcagggcc tgcttgccta catgaactcc
     5701 gagaaagccg acttcatgcg gcgcatcaac gagaccggcg actacgacga cggcatcgtt
     5761 gcggagatgc gccagggcat cgaagcctac aaagcacaag cgaacttcta aggcagcgga
     5821 tatggcggtc ggcaaagaga tcaagaaaaa gatcggcagc gtcgagaaca ccaagaagat
     5881 tacgagcgcg atgcagatgg ttgcggcgag caagatgcgc cgcacccagg agcggatgcg
     5941 ggaaggcaag ccctactcgg aaaagatccg cgcggtcatc ggccatctgg cgaactccaa
     6001 cccggagtac cggcacgtct acatggagcc tcgcgagatc agccggatcg gctacctcgt
     6061 ggtctccacg gacaagggac tctgcggcgg cctcaacatc aacctgttcc gcaccctgat
     6121 ccgggacatg gcggagtggc atgccaaggg caaggagatc gaccttggcc tgatcggcaa
     6181 caaggcggcg gcgttcttcc gctcggttgg tggaaacatc gtcgcggcca tgaacaacgt
     6241 cggcgaggat cctgcgctgg ccgacctcat cggcgggatc aaggtcatgc tcgacgctta
     6301 cgaggaaggc aagatcgacc ggctgttcat cgccagcaac gagttcgtca atacgatgac
     6361 ccagcgcccg gtggtccggc agctcctgcc cctggacccg gaaaccgacg acagcatgag
     6421 gcatcgctgg gactacatct acgagccgga cgcgcgccag ctcatcgagg gcctggttac
     6481 ccgcttcatg gaatcccagg tctaccaggc ggtggtggag aacggtgcct gcgagcaggc
     6541 ggctaagatg gtcgccatga agagcgccac ggaaaacgcg gagaagctca tcgacgagct
     6601 gaccctgatt tacaacaacg ctcggcaggc ggcgatcact caggagattg cggagatcgt
     6661 cggcggggcc gcggcggttt agatagacag attagattga agaggcacaa agcatgagca
     6721 gcggacgaat cgtacagatt atcggcgcgg tgattgacgt cgaattctcg cgggaggatg
     6781 tcccaaaggt ctacgacgcc cttaaggttt cctcaacggg gctcaccctc gaggtgcagc
     6841 agcagctcgg tgacggggtg gttcgtgcca ttgccatggg gccctcggac ggtctctcgc
     6901 ggggtctgga ggtgaccaat acccagcagc ccatctccat ccccgtgggt gaagaaaccc
     6961 ttggccggat catgaacgtg ctcggcgagc ccatcgacga gaaggggcca gttaacgcgc
     7021 agcagaagat gcccattcac cgcaagccgc cctcatacga ggaccagaaa ggcggcaacg
     7081 agattctcga gacgggcgtc aaggtcatcg acctcatctg cccgttcgcc aagggcggca
     7141 aggtgggcct gttcggcggt gccggcgtcg gcaagacggt caccatgctg gagctgatcc
     7201 gcaacatcgc caccgagcac tccggcctgt cggtcttcgc cggtgtgggc gagcggaccc
     7261 gggaaggaaa cgacttctac cacgagatgg aggagtcggg cgtgctcgac aagatctcgc
     7321 tcgttttcgg gcagatgaac gagccgccgg gtaaccgtct gcgggtagcg ctcaccggcc
     7381 tgaccctggc cgagcagttc cgcgacgaag gtcgcgacgt cctcctcttt gtggacaaca
     7441 tctaccgcta cacgctggcg ggaaccgagg tgtccgcgct gctcggccgg atgccgtccg
     7501 cggtaggcta ccagccgaac ctggccgagg agatgggcgt gctgcaggag cggatcacca
     7561 ccaccaagac cggatcgatc acgtccgtgc aggccgttta cgtgcccgcc gatgacctca
     7621 ccgacccgtc tccggccacc acctttgcgc acctggattc cacggtgacg ctctccaggg
     7681 ccatcacgga cctcggcatc taccccgccg tggatccgct ggattccacg agccgtcagc
     7741 tcgacccgct ggtggtcggt gaggagcact atcaggtggc ccggggcgtt cagcaggtgc
     7801 ttcagcgcta ccaggagctc cgcgacatca tcgccatcct tgggatggac gagctttccg
     7861 aggaggataa gcagctggtc tatcgcgcgc gcaagatgca gcagttcttc tcgcagccca
     7921 tgttcgtcgc cgagcccttt accggccagc cgggcgtgta cgtctcccgg gaggatacgg
     7981 tgcgcagctt caagggcatt ctcgagggag aatacgacca ccttccggag caggcgtttt
     8041 acatggtggg caccatcgaa gcggcgatcg agaaagccga gacgcttagc gcctaaggag
     8101 agcggccatg gccatgacct tccattgcga tatcgtcagc gcggagcgcg agatcttctc
     8161 cggccgggtg accatgctga gcgccatcgg gaccatcggt gagctgggca tcctgcccgg
     8221 ccacgccccg ctgctgacgg gcatccgtcc ggggccggtc cggctgcgca tggacaatgg
     8281 cgaggaagag gtcttcttcg cctccggagg gtatctcgag gtccagccag gggtggtcac
     8341 cgttcttgcc gacacggcgc tgcgcgcgga agacatcgac gaggccgccg ctgctgccgc
     8401 tcagcaggat gcggagcggg cattgcggga gcagacggcg gatatcgaat tttccgctgc
     8461 cgctgccatg cttgccgagg cgaccgcgcg gcagaggacg ctggcagagc ttcgcaagcg
     8521 ccggcgctga cgcgccgggc gaatctgccc aaggttcttg tccaatgcag gggtgatctg
     8581 tgtcaccctt tattcgttcc ccggttctat aatcgtcaga gcatagaggt tcggccgccc
     8641 gtgacgcggg tgcggttcgg cccggcctga aaccagggac tgtttctgct caacatggtt
     8701 atgtcttcca gccctcgcct tgaagtcatc gtgctggccg ccggccaggg taagcgcatg
     8761 aactcgcggt taccgaaagt gctgcacccg ctggccgggc gtcccctgct cgcccacgtc
     8821 ctggagaccg ttgacgagct agagcccgcc ggcattcacg tggtcatcgg tcatggcgcc
     8881 gatctggtgc gtgaggccat tggcgcggcg gcgctgcggt gggtggtgca gaccgagcag
     8941 ctgggcaccg gccacgccgt gcagcaggcc ctgccggaca tcgacgattc agccgtggtt
     9001 ctggttctgt gcggcgacgt gccgatggtg acggcccaga cgctcaggcg ctgcgtcacc
     9061 ggcgccggca acggcgcaat ggccgtcatc accgcggagc tgagcgatcc ggcgcagctg
     9121 gggaggatcc tgcgaggtac cgacggcgcc gtgcgcggca tcgtggagta caaagatgcc
     9181 agcgctgccg agaaggccat ctcggagatc aattcgggaa tcatcgccgc gcctgcgggc
     9241 attttgcggg acctgcttgc cgaggtccgc ccggacaacg ctcagggaga gtactacctc
     9301 accgacatcg tggccctcgc ggtcagccgc ggtatcgagg tgctgggtct gaaggcggag
     9361 cggcccgaag aggtgctggg cgtgaatgac cgaaaccagc tggccgtcct cgagcggcgc
     9421 taccagctcg agcgggccgg cgagctgatg gccgccgggg ccaccctggc ggatccgtcg
     9481 cgactggacg tgcgcggcgc ggtcaccgtg ggcgccgact gcttcatcga cgtcaacgtc
     9541 gttttcgagg gcaccgtggt catgggccgg gaagtgagca tcggtcccgg ctcggtcatt
     9601 cgggattctc agctgggcga cggtgtgcgc gtcgaagccc acaccctggt ggacggtgcc
     9661 agggtcgaag ccggttgcag cctggggccc tttgcccgga tccgaccggg cacgcagctg
     9721 ggcgaaggcg tgaagatcgg caacttcgtc gagaccaaga aggccgtgct gggtgcagga
     9781 agcaaggcca gtcacctgac ctatcttggt gatgccatcc tcggggccaa ctgcaacgtg
     9841 ggcgccggca cggtgacctg caactacgac ggtgtcgaca agcaccgaac gaccattggc
     9901 gacggcgtct tcgtaggcac caacagcacg ctggtcgccc cggtcacgct gggcgacgat
     9961 gctttcgtgg cggcggggtc cacgatcacc ggcgatgtcg agaagggcga gctggccgtg
    10021 gggcggggcc gacagcgtaa cgttcagggc tgggtacgac ctggccggcg cgaccgcgcg
    10081 gacgaggatt agcatgagga gcctggcatg tgcgggatcg taggggccgt cgccgaccgc
    10141 aacgtggcgc ccatcctctt ggaaggactt cagcggctgg agtatcgcgg gtacgactcc
    10201 gctggccttg ccgtgatcga tggggcgggt gagatggatt gccggcgtcg cgtcggcaag
    10261 gtcaaggagc tcgccggaga cctgagcgcc cggcgatgcg atggtcacat gggaattgcc
    10321 cacacgcgtt gggcaaccca cggcgttccc agcgagaaca acgcccaccc ccacgtctcc
    10381 aacggccgca tcgcgctggt gcacaacggc atcatcgaaa acttcgagga gctgcgcgaa
    10441 gagcttcaaa gcgagggtta tgcgtttgcg tccgagaccg actcggaggt gatcgttcac
    10501 ctggtggatt catatttcga caagggattg agcctgctcg aggccgtcag gtccgcggtc
    10561 aagcggctgg aaggcgctta cgccatcggc gtcatcgcgc tcgccgaccc tgatcggatc
    10621 gtcgccgcgc gcatgggcag cccgctggtg gttggcaagg ggctgggaga aaacttcatc
    10681 gcttccgacc cccttgcctt gagaccggtg accgaccgat tcgtgttcct ggaggaaggg
    10741 gatctcgtcg agattacccg agatgagatc tccatctgga acgtggagaa cgagtcggtg
    10801 atccgcgcca gcgtccgggt agagctcggt cacgacgacg tggaccgggg caattaccgg
    10861 cacttcatgc agaaggagat ctaccagcag ccggaagtgc tcgcagcgac tctggaaggg
    10921 cgcataggcc gtacccgggt gctcgagccc gcattcgggg tgcaggccgg gcagatcttc
    10981 gacaaagtcc aggcggtgac catcgtggcc tgcgggacga gctactacgc gggctccatc
    11041 gcgcgttact ggatcgagga gctcgcgcgt atcccctgcc aggtggagat cgccagcgaa
    11101 ttccgctacc ggcaggtggc ggttaccccg ggcaacctgt tcgtgaccat ctcccagtcg
    11161 ggtgagaccg cggataccct ggcagcgctg cgcgtggcca aagagctcgg ctacgtctcg
    11221 acgctcacca tctgcaacgt cgccaccagc acgctggtgc gcgagtccga catcgcgctg
    11281 atgacccagg ccgggacaga ggtgggcgtg gcgtcaacca aggcgttcac cacgcagctg
    11341 gccgacctgt tgctgctgac cattctcatt gcccgccggc acgggatgga cgcggccaga
    11401 gagcaggagc tggtggaggc gctcgccggg cttcccaatg ccgttgagca ggtgctcggc
    11461 ctcgacgggc gcatccgcgt tctggcggaa cgcttcatgg accggagcaa cgcgctgttt
    11521 ctcggacgcg ggcccatgtt cccggtggca atggaaggcg cgctgaagct gaaggagatc
    11581 tcctacatcc atgccgaggg ctatcccgcc ggggagctga agcacggccc tctggcgctt
    11641 gtcgacgaga acatgccggt cgttgccgtg gcgccgaacg acgagctgct cgagaaggtg
    11701 cagtccaacc tgcaggaagt gcgcgcgcgc ggcggtgccc tctatgtctt tgccgatgag
    11761 cgcaccggct tcaaagaaga gcccggggtc acggtgatcc cgctgccccc cgttcacgac
    11821 acgctggctc ccatcgtcta cgtcgtgcca ttacaattgc tcgcctatca cgtcgcggtg
    11881 ctcaaaggca cggacgtgga ccagccaagg aatctggcta agtcggtcac ggtggagtag
    11941 tccgttccgg ccctaagccg cgccgggcgg ttccctgaac ccccgtccct tggacaggct
    12001 tagggcgggt agcagtggac gtcacgaaca ttctcgagca tctgaacaag gaccagcggg
    12061 aggcggtctc ggcgcctctg ggcaatgccc tggtggtggc tggcgccggc agcggcaaga
    12121 cgcgggtgct ggttcatcgg at
//
LOCUS       DBNHOX010000634         1909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_502417, whole genome shotgun sequence.
ACCESSION   DBNHOX010000634 DBNHOX010000000
VERSION     DBNHOX010000634.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_502417"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..694)
                     /gene="nthB"
                     /locus_tag="AC16UZ_14010"
     CDS             complement(11..694)
                     /gene="nthB"
                     /locus_tag="AC16UZ_14010"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003410914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HIR6247848.1"
                     /translation="MNGIHDLGGMTCFGPIVREDDEPVFHSDWERRVFAMNMLSMGQF
                     GSLDQARHAIERMDPAAYLSTSYYEHWLAGIEILGRDSGLLMDAEIASGRSEHTSTPS
                     RPPLDPEFVDAMVRSGSPSTRPVSRRPTFDVGDSVRARNLDVRGHTRLPRYVRGKRGV
                     VSAVHGTHVFPDTSAHGLGENPQPLYNVRFEADELWGDNLARRDCVHIDLWEDYLEAS
                     PVEGRSHEQ"
     gene            complement(748..1458)
                     /locus_tag="AC16UZ_14015"
     CDS             complement(748..1458)
                     /locus_tag="AC16UZ_14015"
                     /inference="COORDINATES: protein motif:HMM:NF016300.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR6247849.1"
                     /translation="MTDSRGNFIPGLLVAIGLAVAGYLIGQGFVHARVLDRSVEVKGL
                     AEREVPADTAIWPVQIVAAGDDLQALLVQIEGQSGRVVGFLADLGFTPGEVSVGAPNV
                     LDRQAQNFGDPNTRFRYSVAQTITVFTEAVATVREAGGRLLELGKEGIVVTQNNYDLR
                     TQYLFNGLNDIKPEMIEEATRNARRVAEKFAGDSDSVLGKIRSARQGQFSIQDRDSNN
                     PHIKRVRVVSTLEYYLVD"
     gene            1709..>1909
                     /locus_tag="AC16UZ_14020"
     CDS             1709..>1909
                     /locus_tag="AC16UZ_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247850.1"
                     /translation="MATLDILPTQIDFSALHARMQWYVDERIIPHCVCLVMRGTDVLD
                     YRTLGYMDLESREPLREDAIFRM"
BASE COUNT          335 a          642 c          584 g          348 t
ORIGIN      
        1 gttgtcgtgg tcattgctca tgagatctcc cctccacggg cgacgcctcc agataatctt
       61 cccagagatc gatgtggacg cagtcccttc gcgcaaggtt gtcgccccac aactcgtccg
      121 cctcgaagcg cacgttgtac agcggctgag gattctcgcc cagaccatgg gccgacgtgt
      181 cggggaagac atgcgtgccg tgaacggcgc tgacgacgcc ccgctttccc cgaacgtacc
      241 ggggaaggcg ggtgtgtccc cgaacgtcca gattccgcgc gcggaccgag tcgcccacgt
      301 cgaaggtcgg ccgtctgctc accgggcgtg tcgatggcga tccggagcga accatcgcgt
      361 ccacgaattc gggatccagc ggcgggcggg acggcgtgga ggtgtgctcg ctcctcccgc
      421 tggcgatctc agcatccatc agcagtcccg agtcccttcc caggatttcg atcccggcaa
      481 gccagtgctc gtagtaagac gtgctcagat aggctgccgg atccatccgc tcgatggcgt
      541 gccgcgcctg atcgagggag ccgaactgcc ccatgctgag catgttcatc gcgaagacgc
      601 ggcgctccca gtccgagtga aagacaggct cgtcgtcttc ccgaacgatg ggcccgaagc
      661 acgtcatccc gccgaggtcg tgaatgccgt tcaatgtgtc ctcctttagc cgccgacagc
      721 agacgggcgc ggtaaatgcc cggccactca gtccaccaga tagtactcga gcgtagatac
      781 cacgcggacg cgcttgatgt gcgggttgtt gctgtcccgg tcctgaatcg aaaactgacc
      841 ctggcgggcc gatcggatct tgccgagtac gctgtccgag tccccagcaa acttttccgc
      901 aacgcgccgg gcattccggg ttgcctcttc gatcatttcc ggcttgatgt cgttgaggcc
      961 attgaagagg tactgcgtgc gcaggtcgta gttgttctgg gtcaccacga tgccctcttt
     1021 gccgagctct agcagccgcc cgccagcctc ccgcacggtc gcgaccgctt cggtgaacac
     1081 ggtgatcgtc tgggccaccg agtagcggaa gcgggtgttg ggatctccga aattctgggc
     1141 ctgacgatcg agcacgttgg gcgcgccgac gctgacttcc cccggcgtga agccgagatc
     1201 ggcgagaaac ccgacgaccc gcccgctctg cccctcgatc tgcaccagaa gcgcttgcag
     1261 atcgtcccca gcggccacga tctgcaccgg ccagatggcg gtgtctgccg gcacctcgcg
     1321 ctcggccaga cccttgacct ccacgctgcg gtctaaaacc cgcgcgtgga cgaatccctg
     1381 acctatcagg tagccggcga cggcgaggcc gatggcaacg agcagcccgg gaatgaaatt
     1441 gcccctcgaa tcggtcatgc gtacgaaatc cgcggcaagc tggcccgcca tcatccacta
     1501 tcaggcgggg tcgcttccag cccggtgggc ggcttcaagc tcggtgacga ataccgctgc
     1561 cgcggacgcc gcatcgagag taatggccga gttcccggaa cgtccgggac ggtacttgca
     1621 cgtaccattc tgcccggcca cccaacacac gccattctgg gacggctggc ggtagaatcg
     1681 tccgctggcc gcgcccggga gcgacatcat ggcaacgctc gacatcctgc cgacgcagat
     1741 cgacttctct gccctgcacg cccggatgca gtggtacgtc gatgagcgga tcatccctca
     1801 ctgcgtctgc ctcgtgatgc ggggcaccga cgtgctcgac taccgcacgc tcggctacat
     1861 ggatctggaa tcccgcgagc ctctgcgcga ggacgcgatt ttccgcatg
//
LOCUS       DBNHOX010000635         3324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_180598, whole genome shotgun sequence.
ACCESSION   DBNHOX010000635 DBNHOX010000000
VERSION     DBNHOX010000635.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_180598"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /locus_tag="AC16UZ_14025"
     CDS             complement(<1..1278)
                     /locus_tag="AC16UZ_14025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6247851.1"
                     /translation="MSGPVLTSSFGLRMSAFTALYLAQGVPIGLFSIALPAWLVEQGA
                     DAADIAALVAVTGLPWAFKLIAGPFMDRFSFPAMGRRRPWVMGAQAGFTVSMVSLALV
                     SGPSDGLWPIMVIGFVINTFAAVQDVAVDGMAIDVLPISERGRANAFMAFGQAAGFSG
                     FGALDGYLLSRFGLGVTAIVSAAAIGAVLVFVAVTREREGERLLPWTAGMATAREIAP
                     ARSMLEILRDILRVLLLPMSLLLIAVEFLARAGSGVGLAIMPVLAVDELGYASEVYAY
                     WLGLMGGLSAFVGIFCGPAIDRLGARKVLVVGLLGGSLVTVAYALAAGARGTAGFDLT
                     MLAAAQIFGQVFFVSMIAMFMGICWARVAATQFAIYMSLANLARSAGAGAFALVATDL
                     SVFDALYLVAALSVAAVLLLQRFDPAAHAARLAL"
     gene            1522..3021
                     /locus_tag="AC16UZ_14030"
     CDS             1522..3021
                     /locus_tag="AC16UZ_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247852.1"
                     /translation="MSAWLIATGDIVNNDGMLYLSAAERIAQGDWEAARGLYGWLFYP
                     WLIAVISGIAPLGLEASAHLVDAAMAAVMVYAFLSIVRELGGDRKVELIAAFVIVFHP
                     YLNDSRAEILRDHGYWALYLLATFYFLKCHRNPSWPNALSWACLMALATLFRIEGSVI
                     LVALPFLLLLKRDELPARRLRAFGRAYTLNALVLAAVAVWAAVEPDFTDQAGRLLDPI
                     RKLNQLFASVGEGFQIKAQRLHDAVLTGYTDQYAVPALLAVVFLIMTDNVVRTLSPLY
                     VVLPAFRRFRARLTFPHGVPMILASLCGLHLLSALVFLVPHYYMSSRHVVAASLTLLV
                     TIPFVLASVHDRWIQRGRGGRFQRWPYPLIAAALVFMAADGLLSVGGASKRYIREAGA
                     WLQQNVSQDTRLYSNHRKVLFYGGRPLEFEGPPKAPLAANWISERAWRRYDYVALWER
                     GNDGEDAQVLAEAAELELVASFANDEGDRVLIFSVGPSAAPTRAAPISP"
     gene            complement(3053..>3324)
                     /locus_tag="AC16UZ_14035"
     CDS             complement(3053..>3324)
                     /locus_tag="AC16UZ_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247853.1"
                     /translation="MVAGLPVLTTANCGYAPRVERAGGGRVVPEPFAQERLNAALSES
                     LERGRLEEWSRNGIDYGRNEDLYSRPETAADIIESVIEVKAAARR"
BASE COUNT          599 a         1118 c         1046 g          561 t
ORIGIN      
        1 gagcgccagc cgggcggcgt gagccgcagg atcgaatcgc tggagcagca aaaccgcggc
       61 gacgctcagc gctgcgacca ggtagagcgc atcgaatacg ctgagatccg tcgcgacgag
      121 cgcaaacgcg cctgcgcccg ccgacctggc caggttcgcc agcgacatgt agatggcaaa
      181 ctgggtagcg gcgactcttg cccagcagat gcccatgaac atggcgatca tcgacacgaa
      241 gaagacctga ccgaatatct gggcagcggc cagcatggtg agatcgaatc cggctgtacc
      301 cctcgccccc gccgccagcg cataagcaac cgttaccagc gagccgccga gcaggcccac
      361 cacgagcacc ttccgggcgc ccaggcgatc gatggcgggc ccgcagaata tgccgacgaa
      421 ggcgctcagc ccgcccatca atccaagcca gtaggcgtaa acttccgatg cgtaacccag
      481 ctcgtctacg gccagcacgg gcatgatggc caacccgact cccgaacccg cgcgcgcaag
      541 aaactccacg gcgatgagaa gcaggctcat gggcagcagg agcacccgga gaatgtccct
      601 gagaatttcc agcatcgatc gggccggagc gatctcccgg gcagtcgcca tgcccgccgt
      661 ccagggcagc agacgctccc cttcccgctc ccgtgtcacc gcgacgaaga ctaagaccgc
      721 gccgatggct gcagccgata cgatggcggt gacgcccaga ccaaaacggc tgagcaggta
      781 gccgtccagc gcaccgaatc ccgagaagcc cgctgcctgg ccgaacgcca tgaaagcgtt
      841 cgcccggccc cgctcgctga tcggcagcac gtcgatggcc atgccgtcga cggccacgtc
      901 ctgaacggcg gcgaacgtgt tgatcacgaa gccgatcacc atgatcggcc agaggccatc
      961 cgaagggccg gacaccaggg ccagggagac catggatacg gtaaagccgg cctgggcacc
     1021 catgacccag ggtcgacgcc gacccatggc ggggaaactg aagcggtcca tgaagggccc
     1081 ggcgatcagc ttgaaggccc agggaagccc ggtgacggca acgagcgcag cgatgtccgc
     1141 agcatccgcg ccctgctcca cgagccatgc cggaagagcg atcgagaaca gaccgatggg
     1201 tacgccctgc gccaggtaca gggcggtgaa tgccgacatg cgcagcccga aggacgaggt
     1261 gagaaccggc ccgctcacag ggacacgctc ccacgatcgt gacgacgctt attttcctga
     1321 cccgcgtctt ccaatatgct gattccctgg taagacaaga tgagcaacgg ctcgaagcgc
     1381 cgcgctgccg agcttaaccc atagaacgtg acggctccca aacggacacc cgcctctaaa
     1441 acggacgcgg ccggatctat cggagtttcc tggcgactgg ccagcgacat ccggacgctg
     1501 gccgttctcg caagcctcgt ggtgtcagcc tggctgatcg ccaccggcga catcgtcaac
     1561 aacgacggca tgctttactt gagcgccgcg gagcggatcg cccagggtga ctgggaggcg
     1621 gcacgtgggc tgtatggctg gctgttctac ccgtggctca tcgccgtcat cagcgggatc
     1681 gcgccattgg gccttgaagc gtcggcccat ctcgtcgacg cggccatggc agcagtcatg
     1741 gtgtatgcct tcctcagcat cgtccgggaa ctcggcggcg atcgcaaagt cgagctcatc
     1801 gccgctttcg tgatcgtctt ccacccctat ctgaacgact cgcgcgcgga gattctgcgg
     1861 gatcacgggt actgggccct gtacctgttg gcaaccttct acttcctgaa atgccatcgc
     1921 aatccttcct ggccgaacgc cctcagctgg gcttgcctga tggccctggc cacgctcttc
     1981 cgcatcgagg gttccgtgat cctcgtggct ttgccgtttc ttcttctgct caagcgcgac
     2041 gagctacccg cgcggcgcct gagggcattc gggcgggcgt acaccctgaa cgctctcgtc
     2101 ctggcagccg tggccgtctg ggccgcggtc gagcccgact ttaccgacca ggccggtcgg
     2161 ctgctggatc caattcgcaa gctgaaccag ctgtttgcgt ccgtcggcga gggcttccag
     2221 atcaaggccc agcgtcttca cgacgccgtg ctgaccgggt acacggacca gtacgctgtg
     2281 cccgctctgc tcgcggtggt gttcctgatc atgacggaca acgtcgttcg tacgctgtcg
     2341 ccgctttacg tcgtcctgcc ggcattccgg cgcttcaggg cccgcctgac gttcccgcac
     2401 ggcgtcccga tgattctggc ttctctgtgc gggctgcatc tactgagtgc cttggtcttt
     2461 ctggtgcccc actactacat gtccagtcgc cacgtggtcg cggcgtcgct gacgctgctg
     2521 gtgacgatcc ccttcgtcct cgcctcggtg cacgatcggt ggatccagcg aggtcgtggc
     2581 ggaaggttcc agcgctggcc ctacccgctc atcgcagcgg cgctggtgtt catggcggcc
     2641 gacggcctgc tatccgttgg cggggcgtcc aagcggtata tccgggaggc cggcgcctgg
     2701 ctgcagcaga acgtttccca ggacacccgg ctgtacagca atcatcgaaa ggtcctgttc
     2761 tacggcgggc gtcccctgga attcgaaggg ccgccgaaag cgcccttggc ggcgaactgg
     2821 atctccgaac gggcatggcg gcgctacgac tacgtcgcgc tgtgggagcg cggcaacgat
     2881 ggggaggatg cgcaggtgct tgcagaggca gcagagctag agctcgtcgc gagctttgcc
     2941 aacgatgaag gggaccgggt gctgattttc tcggtagggc cctcggcagc cccgacccgg
     3001 gcggccccta tcagtccctg aaggtgacaa gcgcgttggt gacaccggac gctcagcgcc
     3061 gcgctgcggc cttgacttcg atcaccgact cgatgatgtc cgcggcggtt tccggccggc
     3121 tgtagagatc ctcgttgcgc ccgtaatcga taccgttgcg gctccactcc tcgagtcttc
     3181 cccgctccag gctctctgag agcgcggcgt tgagccgttc ctgggcgaag ggttcgggaa
     3241 ctacccgccc gccaccggcg cgctccacgc gaggcgcgta accacagttg gccgtggtca
     3301 gcactggcag cccggccacc attg
//
LOCUS       DBNHOX010000636         5586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1337076, whole genome shotgun sequence.
ACCESSION   DBNHOX010000636 DBNHOX010000000
VERSION     DBNHOX010000636.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1337076"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="AC16UZ_14040"
     CDS             complement(<1..400)
                     /locus_tag="AC16UZ_14040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6247854.1"
                     /translation="MRTYPGLDGSCEADVCIVGGGYTGLSAALELGRRNYDVVVLEAH
                     RVGSGASGRNGGVLGMGQRKDQLELESWLGADAARKLWRIACDANDLVRDRIAEFGID
                     CDLKSGELTAAHRERYEKTLWSYAEHLERDY"
     gene            complement(472..1785)
                     /locus_tag="AC16UZ_14045"
     CDS             complement(472..1785)
                     /locus_tag="AC16UZ_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR6247855.1"
                     /translation="MGKPSEMPDELELLLSDMNGVPRGKTLRGISYSEAHPPHLAEAI
                     FFQTITGGYAAAMETYDPNDRDLLLRPDWSTYVPTPWKPDTGQVICESLDQAGERLPY
                     DPRNVLRRVLDAYESMGLRPVVAPEVEFYLLEAVSSHDLVLTPATGRSGAAEFGGESF
                     SPDALEKFMPIVAEIQQACRQTGLELSAMVHEMGPAQIELNVAHGDALSRADQLFQLK
                     RLIKGCARKHGALASFMAKPLADLPGNGLHLHCSMEDETGRNIFALNRRRAPAVLQHF
                     IAGLQAHLPQAFALVAPNVNSYKRFVRDLSAPINLEWGYDNRTTGFRVPFSDARAGRV
                     ENRVAGADANPYLIIAATLACGLLGMRAAIKPTKPIADDAYGLKPALPEDLGEALRIL
                     EQSEGLIDLLGEAFVEVFVSVKRGELDHFGKTITQWEVGYLGSTL"
     gene            complement(1820..2572)
                     /locus_tag="AC16UZ_14050"
     CDS             complement(1820..2572)
                     /locus_tag="AC16UZ_14050"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR6247856.1"
                     /translation="MSRPVVLIPCDVKRYGEYPFHCVGEKYINAVAHGAGAMPLLMPA
                     WGRGAELRELTDLFDLDAILDRVHGVFLPGSTSNVHPHRYGGADLDMLHDEQRDAVVF
                     PLIERALSRGLPLLAVCRGLQELNVALGGTLHPRIHEVPGYLDHRENDELPRDAQYGL
                     VHAVDAVHGGMLADLVGAKRFLVNSLHAQGIAELGAGLRVEARAEDGLIEAVSMVGGW
                     VLGVQWHPEWHFTDDTASAKLFAGFGEALRAA"
     gene            2650..3468
                     /locus_tag="AC16UZ_14055"
     CDS             2650..3468
                     /locus_tag="AC16UZ_14055"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR6247857.1"
                     /translation="MSNLALISRDVAEILPAAGTDEFAPSVVAMLRDQVNIDEACMIV
                     YPRDESPRIAHRETQKPGDEPNLDTFIKGPFLLDPYYVDAAREGRFGFFPLSHLAPTG
                     FKTSEYYRTYYRFSGIFDECGYLTPIGDGGFANLSLARADGNQPFESSTLRCLADLAP
                     LVTALTQHHWRLLPANPSTEQISLRTQLESALESFGESVLTARERQIINSILHGHTSK
                     AIAQSLAISVETVKLHRKNAYRKLAIRNQSELFYTFIRALRDCSDEYQGGDPLR"
     gene            complement(3480..4994)
                     /locus_tag="AC16UZ_14060"
     CDS             complement(3480..4994)
                     /locus_tag="AC16UZ_14060"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6247858.1"
                     /translation="MELRGGPYDADSDQSGGDNDDADILTIGVKFEYEFDFATLTSNT
                     GYKDHEYYYNEDYDGTPLNLNNYRQDQEGDYFQQELRLTSKGEGPLSWYAGASYYKEE
                     IDTLFEFAGQEDFFCQYYGYYYNYGMTFTGCSDLYAYYGSPFTPSADGVLRETGRIVG
                     DYSGWAAYVDLTYELTQQWDFSVGLRYSDDEKDFTINVPTPDSDLGAYWAYTFATDGD
                     ITGKESWDDLQTRFVARFRPNDDAMLYASYTEGFKSGGFGSFALVDADGVGAGGGDTD
                     LSQSDGYRPNAFQPEVADSYEIGYKGSLAEGRAQLSLTGFYYEYEDLQVVVFDGGAAA
                     VENVGNVEGWGVEGTANFLLGDYVDVYLGLSYLDTEATGLQSICGLEDAEGCEGSSLF
                     WAPEWTGSVVVNGNFPLEAGGALTGRVEGFWESERGGGFEGLKTTEIDSFFNLAVRVG
                     YESEHNWSANLYVENLTNEDTFDGLNNNGGVLPSHFFGPMRPRTFGVSFSYVWD"
     gene            complement(5076..>5586)
                     /locus_tag="AC16UZ_14065"
     CDS             complement(5076..>5586)
                     /locus_tag="AC16UZ_14065"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6247859.1"
                     /translation="GVSGNSQDSFIDAISVRGIRTQDFGVGGDPSAAFFKNDLYEGRN
                     GSAVTSLFDMERAEILRGPQGFLFGRNSIGGAFSVHTAKAEIADRASGYVDVDIGERD
                     HLVVEGAVNLPINDRFAMRLAGYSSNEDGFVKNRFNDEKLIEHEKYALRWSTAFATDR
                     LSLDTMVEY"
BASE COUNT         1037 a         1873 c         1660 g         1016 t
ORIGIN      
        1 cgtagtcacg ctccagatgc tcggcgtacg accacaacgt tttctcgtaa cgctcgcggt
       61 gcgctgccgt gagctcgccg cttttcaggt cacagtcgat cccgaattcg gcgatgcggt
      121 cacgcaccag gtcattggca tcacaggcga tccgccacag cttccgggcc gcatcagcgc
      181 ccagccagga ctcgagctcc agctggtctt ttcgctggcc catgccgagc acgccgccgt
      241 tgcgtccgga cgctcccgag ccgacccgat gggcttccag caccaccacg tcgtaatttc
      301 tgcggccgag ctccagggcg gccgacagcc cggtgtagcc accgccgacg atgcagacgt
      361 cggcttcgca ggatccatcg agaccggggt aggtccgcat tgtctttgcg ctgtggtaat
      421 accaggactc gtcaaacccg accctgggat ctcccccgcc ccgagatcgg ctcacagcgt
      481 gctgcccagg tagccgactt cccactgagt gattgtcttg ccgaaatggt caagctcgcc
      541 acgcttgacc gacacgaaga cctccacgaa cgcctcgccc aggagatcga tgaggccttc
      601 gctttgttcc agaatccgca gcgcttcacc cagatcttcc ggaagcgccg gcttcagacc
      661 gtaggcatcg tcggcgatgg gcttggtcgg cttgatggca gcacgcatac ccagcagtcc
      721 gcaggcgagc gttgcagcaa tgatgaggta agggttcgcg tccgcacctg ccacgcggtt
      781 ctcgacgcgc cctgctcggg catcactgaa gggcacgcgg aagccggtgg tccggttgtc
      841 gtaaccccat tccaggttga tgggcgccga cagatcccgc acgaagcgct tgtaggagtt
      901 cacgttgggg gcaaccaacg cgaacgcctg gggcagatgc gcctgcagtc ctgcaatgaa
      961 gtgctggagc acggcgggcg cccggcgacg attgagcgca aagatattgc gcccggtctc
     1021 gtcctccatg ctgcaatgca ggtgcagccc gttccccggc aggtctgcca gcggcttggc
     1081 catgaagctt gccagggcgc cgtgcttgcg cgcgcagccc ttgatcagcc gcttgagctg
     1141 aaacagctga tccgcgcggg agagcgcgtc tccgtgggcc acgttgagct cgatctgggc
     1201 gggtcccatc tcgtggacca tcgccgagag ctcgagaccc gtctggcgac aggcttgctg
     1261 aatctcggcc acgatgggca tgaacttctc cagcgcatcg gggctgaagg actcgccgcc
     1321 gaactcggca gcccccgacc ttccggtcgc cggcgtcagc accaggtcat ggctggacac
     1381 agcttccaga agatagaact cgacttcggg agcgaccacg gggcgaagtc ccatggactc
     1441 gtaagcatcc agcaccctcc gcagcacgtt acgcggatcg tagggcaggc gttcacccgc
     1501 ctggtcgagg ctttcgcaga tcacctggcc cgtgtccggc ttccaggggg tcgggacgta
     1561 ggtcgaccaa tccggacgca aaagcagatc gcgatcgttg gggtcgtatg tctccatggc
     1621 ggccgcgtac ccgcctgtga tggtctggaa aaagatcgct tccgccaggt ggggcgggtg
     1681 cgcttcggaa tagctgattc cacgcagggt cttgccgcgg ggcacgccgt tcatgtccga
     1741 cagcagcagt tccagttcgt ccggcatttc cgatggcttg cccatccgct ccctcctcag
     1801 tcgaccagcc gggcagcaat cacgctgccc gcaaagcttc gccaaagccg gcgaacagct
     1861 tggcagacgc ggtgtcatcc gtgaagtgcc actccggatg ccactgcacg cccagcaccc
     1921 agccgccgac catggacact gcttcaatca agccgtcctc ggcccgtgcc tccacgcgca
     1981 gcccggcccc cagttcggcg ataccctggg catgcagcga attgacgaga aaacgtttcg
     2041 cgccgaccag gtctgcaagc atgccgccgt gaaccgcatc gacggcatga accagcccgt
     2101 actgggcatc ccgcggcagc tcgtcgttct cccggtgatc cagatagccg ggcacctcgt
     2161 gaatccgcgg atgcagggtg ccgccgagag ccacgttcag ctcctgcaaa cccctgcata
     2221 ccgcgagcag cggcagaccg cgtgacaggg cgcgctcgat gagaggaaac acgacggcgt
     2281 cgcgctgctc gtcatggagc atatccaggt ccgcgccccc gtagcggtgg gggtgaacgt
     2341 tgctcgtaga tcccggcagg aataccccat ggacccggtc gaggatcgcg tccagatcga
     2401 acagatccgt cagctcgcgc agctctgcgc cccgacccca cgccggcatg agcaggggca
     2461 tcgcccccgc accgtgggcg accgcattga tgtacttctc gcccacgcag tggaacgggt
     2521 actcaccata cctcttcacg tcgcagggaa tgagcacgac cggcctgctc atcgcctccc
     2581 tccggctgcg tggcgagtcg gcctggctgg cgataagatg tggtgatcgg cgacggatcg
     2641 tgggcaaaca tgtcgaatct ggcactcatc agtagagacg tcgctgagat cctgccggca
     2701 gccgggaccg atgaattcgc cccgagcgtc gtcgcgatgc tcagggatca ggtgaacatc
     2761 gacgaggcct gcatgatcgt ctatccccgc gacgagtccc cgcgcatcgc ccaccgcgaa
     2821 actcagaaac caggggacga acccaatctc gacaccttca tcaaggggcc cttcctgctc
     2881 gatccgtatt acgtcgacgc agcccgggag ggccgattcg gattctttcc gctttcccat
     2941 ctcgccccca ccgggttcaa gacaagcgag tactaccgaa cctactaccg gttcagcggc
     3001 atcttcgacg agtgcggcta tcttaccccc atcggggacg gcggctttgc gaatctttcc
     3061 ctggctcgcg cagacggcaa ccaaccgttc gagtcctcca cactcagatg cctggcagat
     3121 ctcgcgcctc tggtcaccgc gctcactcag catcattggc gactgctacc cgccaacccg
     3181 tctaccgagc agatcagcct gcgaacgcag ctggagtcgg cgctggagag ctttggcgaa
     3241 tcggttctga ctgcacgcga gcgccagatc atcaactcga tcctgcacgg ccacacgagc
     3301 aaggccatcg cccagtcgtt ggccatctcc gtggagaccg tgaagctcca ccggaaaaac
     3361 gcctaccgaa aacttgccat ccgcaaccaa tccgagctct tctacacgtt catccgcgcg
     3421 ctgagggact gcagcgacga gtaccaggga ggcgatccgc tgcgctagca gcggctcacc
     3481 taatcccaga catagctgaa gctcacgccg aaggtccgcg gccgcatggg gccgaagaag
     3541 tgggaaggca gaacgccacc gttgttgttg agcccgtcga aggtgtcctc gttcgtcagg
     3601 ttctcgacgt aaaggttggc cgaccagttg tgctccgatt cgtagcctac gcgtaccgcc
     3661 agattgaaga aggaatcgat ctccgttgtc ttcaacccct cgaaaccgcc gccgcgttcg
     3721 gattcccaga acccttccac gcggccggtg agggcgcccc cggcttccag cgggaagttg
     3781 ccgttgacca ccactgagcc cgtccactcc ggcgcccaga acaacgagct gccctcgcag
     3841 ccttccgcat cctccagacc gcagatgctc tgcaggccgg tggcttcggt atccaggtag
     3901 gaaagcccca gataaacgtc aacgtagtcg ccgagcagga aattcgccgt tccttcgacg
     3961 ccccagcctt cgacgttgcc cacgttctcc accgccgcgg cgcccccatc gaacacgacc
     4021 acctgcaggt cctcgtactc gtagtaaaag ccggtcaggc tcaactgcgc gcgcccttcg
     4081 gcgagggagc ccttgtagcc gatctcgtag gagtcggcga cctcgggctg gaaggcattc
     4141 ggacgatacc catcgctctg ggagaggtcg gtgtctccac cgcccgcgcc cacgccgtcg
     4201 gcatcaacca gcgcgaagct gccgaaaccg ccggacttga atccctcggt gtagcttgca
     4261 tacagcatcg cgtcgtcgtt ggggcgaaag cgcgccacga atcgcgtctg cagatcgtcc
     4321 cagctctcct tgccggtgat gtctccgtcg gttgcgaacg tgtatgccca gtaggcgccg
     4381 aggtcgctgt cgggggtcgg gacgttgatg gtgaagtcct tctcgtcatc cgagtagcgc
     4441 agcccaacgc tgaagtccca ctgctgtgtg agctcgtagg tcagatccac gtaagcggcc
     4501 catccggagt agtcgcccac gatgcggccg gtctcccgca gcaccccgtc cgcagacggc
     4561 gtaaacggtg atccgtaata ggcatagaga tcagagcatc cggtgaacgt catgccgtag
     4621 ttgtagtagt aaccgtagta ctggcagaag aagtcttcct gaccggcgaa ctcgaagagg
     4681 gtatcgatct cctccttgta gtacgacgcg ccggcgtacc atgacagcgg gccttcaccc
     4741 ttcgaggtca gccgcagctc ctgctgaaag tagtcgccct cctgatcctg ccgatagttg
     4801 ttgaggttca ggggcgtgcc gtcgtagtcc tcgttgtagt agtactcgtg atccttgtac
     4861 ccggtgttcg aggtcagggt cgcaaagtcg aactcgtact cgaacttgac gccaatggtg
     4921 aggatatcgg cgtcatcgtt gtcaccgccg ctctggtccg agtccgcatc gtacgggccg
     4981 cctcgaagct ccacctcgcc gatggcggct tcgaaggtct cccagatgtc gccctcatct
     5041 atggcccggt atacggatcc ggactgctcg cggtcctagt actcgaccat ggtgtccagg
     5101 ctgagccgat ccgtggcgaa cgccgtggac cagcgaaggg cgtacttctc gtgctcgatg
     5161 agcttctcgt cgttgaaccg gttcttgacg aaaccgtcct cgttggagct gtagcccgca
     5221 agccgcatgg cgaagcgatc gttgatcggc aggttgacgg ccccctccac aaccaggtgg
     5281 tcccgctcac cgatatcgac gtccacatag cccgaagcgc gatcggcgat ttccgccttg
     5341 gccgtatgca cgctgaacgc cccgccgatg gagttgcggc cgaacaggaa gccctggggc
     5401 ccacgcaata tctccgcccg ctccatgtcg aagaggctgg tgaccgcaga gccgttgcgc
     5461 ccctcgtaaa gatcgttctt gaaaaaggcg gccgacggat cgccgccgac gccgaagtcc
     5521 tgggtgcgga tgccgcgcac gctgatagcg tcgatgaagc tgtcctggct gttacccgaa
     5581 accccg
//
LOCUS       DBNHOX010000637         1682 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_115557, whole genome shotgun sequence.
ACCESSION   DBNHOX010000637 DBNHOX010000000
VERSION     DBNHOX010000637.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_115557"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1682)
                     /gene="alaS"
                     /locus_tag="AC16UZ_14070"
     CDS             complement(<1..>1682)
                     /gene="alaS"
                     /locus_tag="AC16UZ_14070"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR6247860.1"
                     /translation="ETIGWAWDLVTDVFAVPVEKLWVTVHTSDDESRAIWERKIGVPA
                     ERVISLEENFWAMGDTGPCGPCSELFYDHGPEVAGGPPGSPDEDGDRYIEFWNLVFPQ
                     YDRQPDGELDLLPQPGVDTGMGLERIAAILQGVHSNYEIDAFRSLLSAAGGLAGINDP
                     RDCLTNASVRVISDHIRSSAFLIADGIMPGNEDRSYVLRRIIRRALRHGYKLGIREPF
                     FHRLVKPLAEEMGEAYPEIVERQADVARALLREEEKFAETLGHGMALLNQTIDDLTGS
                     EIPGEVVFQLYDTFGFPTDLTADVARERHLTVDVDGFDKAMKAQQERSKASARFSANV
                     AQRIYTEGKVEFSGYELAEEQSTVVGVFGAEGQAADALGAGENGIVVLDRTPFYAESG
                     GQVGDTGVLQGDGFQFQVTDTRPGGDQHLHIGSVTKGTVTVGQTLTARIDAHRRRAIV
                     LNHSATHLMHAALRDELGSHVQQKGSLVAPDRLRFDFSHPEPMSREQLRRVEAAVNAQ
                     IRENSEVLAEVVGYDDAINRGAMALFGEKYGDQVRVLTMGEGYSVELCGGTH"
BASE COUNT          299 a          578 c          489 g          316 t
ORIGIN      
        1 cgtgggtgcc accgcagagc tccaccgaat agccttcgcc catggtgaga acccggacct
       61 gatccccgta cttttctccg aaaagagcca tggcgccgcg attgatggcg tcgtcgtacc
      121 cgaccacctc ggccagcact tcgctgttct cccggatctg cgcgttgacg gcggcttcga
      181 cgcgccggag ctgctcccgg ctcatcggtt cgggatggga aaagtcgaac ctcagccgat
      241 ccggcgccac cagagatcct ttctgctgga cgtggctccc gagctcatcg cgaagagcgg
      301 cgtgcatgag gtgagtggca gagtggttga gaacgatggc acgacggcga tgggcgtcga
      361 ttcgcgccgt taacgtctgg ccgaccgtca ccgtgccttt cgtgaccgaa ccgatgtgaa
      421 ggtgctgatc cccaccgggc cgggtatccg tcacctgaaa ctggaacccg tcaccctgca
      481 acacgcccgt atcgcccacc tgaccgcccg actccgcata aaacggggtc cgatcgagaa
      541 cgacgatgcc gttctctcct gcccccagcg catcggcagc ctggccttcc gcaccgaaca
      601 cgcctaccac ggtggattgc tcctccgcga gctcgtagcc ggaaaactcc acctttcctt
      661 cggtgtagat cctctgcgca acgttggcgg agaaccgtgc cgacgccttg cttctctcct
      721 gctgcgcttt catggccttg tcgaagccgt ccacgtccac ggtcaggtgg cgctctctgg
      781 caacgtccgc cgtgaggtcc gtcgggaagc cgaaagtgtc gtagagctgg aagaccactt
      841 cgccgggaat ctcgctgccc gtcaggtcgt cgatggtctg gttgagcagc gccatgccgt
      901 gaccgagggt ctcggcaaac ttttcctcct ctcgcagcag agccctggcc acatcggcct
      961 gtcgctccac gatctcggga tacgcctcgc ccatctcctc cgcgagcggc ttgacgagcc
     1021 tgtggaagaa cggctcccgg attcccagct tgtatccgtg tcgcagcgcg cgacgaatga
     1081 tccgccgcag aacgtagctg cgatcctcgt tgccaggcat gatcccatcg gcaatcagaa
     1141 aagcgctcga gcgtatgtgg tcggatatca cccgcacgga ggcgttggtc agacagtcac
     1201 gcggatcgtt gatgcccgcg agcccgcctg ctgcgctgag cagcgaccgg aaagcgtcga
     1261 tctcgtagtt gctgtgcacc ccctgcagga tggccgcgat gcgctcgagc cccatgccgg
     1321 tgtctacacc cggctgcgga aggagatcca gttctccatc gggctgccgg tcgtactgcg
     1381 gaaacaccag gttccagaac tcgatgtaac gatccccatc ctcgtcggga gatcccggcg
     1441 gcccaccggc cacctcgggc ccgtgatcgt aaaacagctc gctgcaaggc ccgcagggtc
     1501 ccgtgtcacc catggcccag aagttctctt ccagcgagat gacccgctcc gcgggaacgc
     1561 cgattttccg ctcccagatg gccctcgact cgtcatccga ggtgtgaacg gtcacccaca
     1621 gcttctctac gggaacagca aatacgtccg tgacgagatc ccacgcccag ccgatggtct
     1681 ct
//
LOCUS       DBNHOX010000638         2864 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1271650, whole genome shotgun sequence.
ACCESSION   DBNHOX010000638 DBNHOX010000000
VERSION     DBNHOX010000638.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1271650"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..834)
                     /gene="mltB"
                     /locus_tag="AC16UZ_14075"
     CDS             complement(73..834)
                     /gene="mltB"
                     /locus_tag="AC16UZ_14075"
                     /inference="COORDINATES: protein motif:HMM:NF024798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HIR6247861.1"
                     /translation="MRYAFLWATTLLLLFGSAHADFAGRAEVREYIAEMVREHGFGEE
                     ELADVFADADRKESILEAIARPAERVLKWHEYRRIFLGEKRISQGIEFWREHAETLAR
                     AEAAYGVPPEYVVAIIGVETRYGRNRGGYRVVDALTTLAFDYPPRSAFFRDELTHFLL
                     LAREEGMDPRDPKGSYAGAMGYGQFIPSSYRSYAVDFDGDGLRDIWSNPTDAIGSVAN
                     YFKRHGWQSAQPVVFPVAAEGDGLSAVANKTLTSS"
     gene            complement(891..1976)
                     /gene="rodA"
                     /locus_tag="AC16UZ_14080"
     CDS             complement(891..1976)
                     /gene="rodA"
                     /locus_tag="AC16UZ_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR6247862.1"
                     /translation="MGAGRRLVQIHLDPLLLLLLLAIVGYGLVVLYSALNGNEELFSN
                     QAVRIGLALSVMLVAAQLEPRFYLRWSPVVYGVGLVLLVLVLLIGVKVKGSQRWLDLP
                     GLPRFQPSELMKIAVPMMVAWYFHERRLPPSFRDVCVAIVIIGLPAGCIILQPDLGTG
                     ILVACAGFAVVVLAGVYWRWIAFAAFGIAALAPGLWFLLQDYQRQRILTLFDPESDPL
                     GAGWNIIQSTTAIGSGGVWGKGLMQGTQSRLDFLPESQTDFIIAVIGEELGLVGIASL
                     LVLYLVVVARGLYIATRAVNTYQRLLAGALTLTFFVYVFVNIAMVSGLLPVVGVPLPL
                     VSYGGTSALTLMASFGIIMAIRTERAW"
     gene            complement(2018..>2864)
                     /locus_tag="AC16UZ_14085"
     CDS             complement(2018..>2864)
                     /locus_tag="AC16UZ_14085"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR6247863.1"
                     /translation="QIDDQGWYQLPGQERIYRDWSWAVDDSGGQGLTNLTRAIYRSSN
                     VYFYDLATRLDIDRIATFAAQFGYGQRTAVDIPDASKGLLPTRVWKKGAKGEIWYPGD
                     NVNIGIGQGDLLATPLQLATVAAVVANRGEFVRPQMLLSSDRPLLEFDPARPMPRVEG
                     PSAEDWERMVDAMEDVVHRGNRGVGQNGTAWAYIGRNIPYRMAGKSGTAQVVEIKQGE
                     EYDEEELEEYQRKHAWFIAFAPADDPKIAVSVLVENGGGGSSVAGPVAREVIDAFMLP
                     QLAAR"
BASE COUNT          568 a          953 c          880 g          463 t
ORIGIN      
        1 ctgcggtatc ttcggcgaga ccggcggcct cgccggccgg cctcatgccc atatccttaa
       61 gctcaccgac cgtcagcttg aggtcagggt cttgttggcc acggcagaga gcccgtcgcc
      121 ctccgcggcg acgggaaata ccaccggttg cgcgctttgc cagccatgcc gcttgaagta
      181 gttggccacg ctgccgatgg cgtccgtggg gttgctccag atatcccgca gaccgtctcc
      241 gtcaaagtcc acggcatagc tgcgatagct ggaaggaatg aactgaccgt atcccatggc
      301 gccggcatag gagcctttcg gatcccgcgg atccatgccc tcctcccggg cgagcagcag
      361 aaaatgggtg agctcgtcac gaaagaaagc ggagcgcggc gggtagtcaa aggcgagcgt
      421 cgtcagagcg tccacgaccc ggtatccacc ccgattgcgc ccgtagcgag tctccacccc
      481 gatgatcgcc acgacgtact cgggcggcac gccataggcc gcttctgctc gtgccagggt
      541 ttcggcgtgc tcgcgccaga actcgatacc ctgagagatg cgcttctctc cgaggaatat
      601 ccgccgatac tcgtgccact tcagcacccg ctcggcggga cgggcaatgg cctcgaggat
      661 gctttctttg cgatcggcat cggcaaagac gtcggcaagt tcctcctccc cgaaaccgtg
      721 ctcccggacc atctcggcaa tgtactcgcg tacctcagcc cgaccggcaa agtccgcgtg
      781 cgcggagccg aacaggagaa ggagggtggt tgcccagaga aaagcgtatc gcatgaatct
      841 acctatccga acgtgtccaa tgtgaaagaa gggacccgaa accaggcgca ctaccatgcc
      901 ctctcggtgc ggattgccat gatgatgcca aaactcgcca tgagcgtgag cgcagacgtg
      961 cctccgtagc tgaccagcgg cagcggcacg ccgacgaccg gcagcagccc gctcaccata
     1021 gcgatgttga cgaacacgta aacgaagaac gtcagcgtca gcgcgcctgc cagcaggcgc
     1081 tgatacgtat tcaccgcgcg cgtcgcgatg tacagccccc gggcgaccac gacgagataa
     1141 agaacgagca gagaggcaat gccaaccagc ccgagctcct cgccgatgac ggcgatgatg
     1201 aaatccgtct ggctctcggg caggaaatcc aggcggctct gcgtgccttg catcagcccc
     1261 ttgccccaca cgccgccaga cccgatggcc gtcgtcgact gaatgatgtt ccagccggcg
     1321 cccaggggat cgctttcggg atcaaacagg gtgaggatcc gctgtcgctg atagtcctga
     1381 agcaggaacc aaaggcccgg ggcaagggcg gcgatgccga acgcggcgaa ggctatccat
     1441 cgccagtaga cgccggccag caccaccacg gcaaagcccg cgcaggccac gaggatgccc
     1501 gttcccagat ccggctgcag gatgatgcaa ccggccggca ggccgatgat gacgatggcg
     1561 acacagacgt cgcggaagct cggcggcagg cgtcgctcat ggaagtacca ggccaccatc
     1621 atgggcacgg cgatcttcat gagctccgaa ggctggaatc tgggcagtcc aggcaggtcg
     1681 agccaccgct gggagccttt gaccttcaca ccgattagga ggacgagcac gagaagcacc
     1741 aggcccacgc cgtagacgac gggcgaccag cgcagataga agcgcggctc cagctgcgct
     1801 gccaccagca tcaccgagag cgccaggccg atgcgcacag cctgattcga aaacagctcc
     1861 tcgttgccgt tcagggcgct gtagaggacc accagtccgt agcccacgat ggccagcaga
     1921 agcagaagca gaagcgggtc gaggtgaatc tgcaccagac ggcgacctgc gcccatggcc
     1981 cggccgccgg ggaggtgcct gacgaagtcc tgattgctca ccgcgcggcc agctgaggaa
     2041 gcatgaaagc atcgatgacc tcacgggcaa cgggtcccgc caccgagctg ccacccccgc
     2101 cattctccac caggacggac accgcgatct tcgggtcgtc ggccggcgca aacgcaatga
     2161 accaggcgtg tttgcgctgg tactcctcga gttcctcctc gtcgtactct tcgccctgct
     2221 tgatctccac cacctgggcg gtgccggact tgccggccat tcgatacgga atgttccgac
     2281 cgatgtaagc ccaggcggtc ccgttctgac ccaccccgcg attgccccgg tgcaccacgt
     2341 cttccatggc atcgaccatg cgctcccaat cctcggcgct cggcccttct acccggggca
     2401 tcggtctggc cggatcgaac tccaggagcg gcctgtcgct ggaaagcagc atctgagggc
     2461 gaacaaactc tccgcggttg gccaccacgg ccgccacggt ggcgagctgg agcggggtgg
     2521 cgagcaggtc gccctgaccg attccgatgt tgacgttgtc acccggatac cagatctcgc
     2581 ccttcgcgcc tttcttccat acccgcgttg gcagcaggcc cttgctcgca tcgggaatgt
     2641 cgaccgcggt gcgctgcccg tagccgaact gggcggcgaa ggtggcgatc cggtcgatgt
     2701 ccagccgggt ggcaagatcg tagaagtaga cgttggaaga ccggtaaatg gctcgcgtca
     2761 ggttggtgag gccctgacct cccgagtcgt ctaccgccca gctccagtcc cggtatatgc
     2821 gctcctgccc gggcagctgg taccacccct gatcgtcgat ctga
//
LOCUS       DBNHOX010000639         2085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_502665, whole genome shotgun sequence.
ACCESSION   DBNHOX010000639 DBNHOX010000000
VERSION     DBNHOX010000639.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_502665"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="AC16UZ_14090"
     CDS             complement(<1..649)
                     /locus_tag="AC16UZ_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247864.1"
                     /translation="MDTNPRKGGSPLGRVLLPFLLLAAATASAVPDSGPVVRLFPTTV
                     LEDIRETGQVAKEMENSLQEVINRLDLQHQLYVESLCEGSDGDQGCERMAKQLGATYL
                     EMLTIMGDRLPEMELAVNNTRASLEKRLREELGHRTTPTSLQNALLGTTPASSAAAEQ
                     PALRGRSGVRLSDRFAQYYALVATQREGRGRSLAVVAADIYLDMEEASRLIAATQE"
     gene            complement(712..1344)
                     /locus_tag="AC16UZ_14095"
     CDS             complement(712..1344)
                     /locus_tag="AC16UZ_14095"
                     /inference="COORDINATES: protein motif:HMM:NF017269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HIR6247865.1"
                     /translation="MRAPALGLGLSLCVAAAPGMAGHSDGFPQAAPAGSHTHYTYADV
                     VGVEPLLEYRTVTEPRRDCRVAGYAAANSGTRSHRYTGPGHPDAHDHYQDHHDHGSPV
                     AAVIGGVVGGLLGSQFGSGSGKKAMTVAGALAGASVASSVSRSRSRHAGYTPRRCTTV
                     MTSREVEEVAGYRVRYVYQGEEFTKVVASDPGDRVRVQVQVTPAVGEPDW"
     gene            1538..>2085
                     /locus_tag="AC16UZ_14100"
     CDS             1538..>2085
                     /locus_tag="AC16UZ_14100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6247866.1"
                     /translation="MTKSDHHQILIIGGGAAGITVAASLKRRQGRHDLDIGIVEPSEH
                     HYYQPALTLVGAGTYPLEKTRRAESRLIPPGVAWHRDSATGFDPESNSVTLASGARLS
                     YDYLVVCPGLELNWERIDGLRETLGSNGVCSNYSPDHVEYTWQCLQSLQEGATALFTQ
                     APLPFKCPGAPQKIAYLAADHL"
BASE COUNT          352 a          699 c          687 g          347 t
ORIGIN      
        1 cctcctgggt cgcggcgatg aggcgggaag cttcttccat gtccagatag atgtcggcgg
       61 cgaccaccgc cagcgagcgg ccacggccct cacgctgggt cgcgaccagg gcgtagtact
      121 gtgcaaacct gtcggaaagc cgcaccccgg aacggccgcg aagcgccggc tgctcggcgg
      181 cggcgctgct tgcaggcgtg gtccccagca gggcgttctg cagcgatgtg ggggtggttc
      241 gatgcccgag ctcttcccgc aggcgctttt ccaggctcgc gcgggtattg tttacggcca
      301 gttccatctc cggaagccgg tcacccatga tggtgagcat ctccagatag gtcgcgccaa
      361 gctgtttggc catgcgctcg cagccctggt cgccgtctga gccctcgcag aggctctcca
      421 cgtatagctg atgctgcaga tcgagccggt tgatgacctc ctgcaggctg ttctccattt
      481 ctttggcaac ctggcctgtc tcacgtatgt cctcgagcac ggttgtcggg aagagccgaa
      541 cgaccgggcc cgaatccggg acggcggaag ccgtggcggc ggcaagcagc aggaacggca
      601 gcagcacgcg gccgagcggg ctgccgccct ttcttgggtt ggtatccatg ggcatatcct
      661 ctttgcttgc gcgccgcaaa acggcgcagg tgtctggacg gaagagagcg ctcaccaatc
      721 tggctccccg acggcgggag tgacctgaac ctgaacgcgc acgcgatcgc ccgggtctga
      781 agccaccacc ttggtgaact cttctccctg atagacgtag cgcacccggt agccggcgac
      841 ctcttccacc tcgcgggacg tcatgaccgt cgtgcagcgc cgcggcgtgt agccggcatg
      901 gcgcgaccgg gaccggctga cgctgctggc aaccgaggcc cccgccagcg ctccggcaac
      961 ggtcatcgcc ttcttgccgc tgccgctgcc gaactggctg cccagcagcc cgcccacgac
     1021 gccgccgatc acggcggcaa ccggactgcc gtgatcgtgg tgatcctggt agtggtcgtg
     1081 ggcgtccgga tggccaggcc cggtgtatcg gtgcgaacgg gtgccggaat tagccgcggc
     1141 gtaccccgca acccgacaat cccttcgggg ctcggtgacc gtgcgatatt ccaacagggg
     1201 ctccacgccg acgacgtcgg cgtaggtgta gtgagtgtgg gatccggcgg gggcggcttg
     1261 cggaaaaccg tcgctgtggc ccgccatgcc gggtgccgcg gcaacgcaga gactcaagcc
     1321 gaggccaagg gctggtgcgc gcatgtttcc tccggaactc ccaaatctgt cgagactcga
     1381 atgttatggg agctcagcta aacagaagct gaacggggcg gcccgaccgg ggcgggtttg
     1441 ccctaccggg cgcacccgct tctccagccc cccggagcgg cgtatgctca tccagactcc
     1501 agcggcaccg ctcgcaggac ccggccggag agccaacatg accaagtcag accatcatca
     1561 gatcctgatc atcggcggcg gggcggcagg aattacggtt gccgccagcc tcaagcgcag
     1621 acagggacgg cacgacctcg acatcggaat cgtcgagccg tccgagcatc actactacca
     1681 gccggcgctg acgctcgtcg gcgcgggcac ttaccctctc gagaagaccc gccgggcgga
     1741 gtcgcggctg atcccgccag gcgtggcgtg gcatcgggac tcggcaaccg gtttcgatcc
     1801 ggagtccaac tcggtgaccc tggcctccgg ggcgaggttg tcctacgact acctggtggt
     1861 ctgcccgggc ctggagctca actgggagcg aattgacggt ctccgggaaa ccctggggag
     1921 caacggcgtc tgcagcaact actcgcccga ccacgtcgag tacacgtggc aatgcctgca
     1981 gtcacttcag gaaggcgcca ccgcgctgtt cactcaagcg ccactgccgt tcaagtgccc
     2041 gggagcgcct cagaagatcg cctacctggc cgccgatcat ctgca
//
LOCUS       DBNHOX010000640         3232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_758256, whole genome shotgun sequence.
ACCESSION   DBNHOX010000640 DBNHOX010000000
VERSION     DBNHOX010000640.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_758256"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..905)
                     /locus_tag="AC16UZ_14105"
     CDS             complement(36..905)
                     /locus_tag="AC16UZ_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6247867.1"
                     /translation="MKDFAGRTAVITGGASGIGLAMAQRFAEERMNVVLADIEENALR
                     AAVEHLEARQHRALGVVTDTMRRDAIEALLERAVAEFGNVHVLCNNAGVVVGGEHVPV
                     WEIPDADWEWVMGVNFYGVLYGLQTFVPHMLAHGEPGHIVNTASVAALMPGGDTYGVS
                     KHGVLVISEALHRDLRAAGSALGASVLCPGWVDTKIAEAERNRPTAMTSAKNPEGHGL
                     PLGDTLKQGMPPEVVAEKVLNSIREERFYILPHAGWDEVVLGRVDAIVARGAPYELDR
                     EALVAKRGLGEEV"
     gene            1006..2388
                     /locus_tag="AC16UZ_14110"
     CDS             1006..2388
                     /locus_tag="AC16UZ_14110"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR6247868.1"
                     /translation="MSGKTRTEKDSMGALEVPADALWSAQTQRAVENFPISGIRMPGA
                     FIRSLGLIKAAAAHVNADLGVLPAELALPIVEAAERVAAGSVDDHFPVDVFQTGSGTS
                     SNMNANEVIARLASDASGLEVHPNDHVNAGQSSNDVIPTAIHVSAVLATREDLLPALA
                     HLAGVLRERAAGLQGVVKTGRTHLMDAMPIRLGQEVGAWATQVEQDIARIRDTLPRCE
                     RLAQGGTAVGTGVNAHPEFAVRFARRLSDVTGVPFLPSEDLPASLASQDAAVELSGQL
                     KVTAVSLMKIANDLRWMNSGPLAGIAEIMLPALQPGSSIMPGKVNPVIPEAIAMVCAQ
                     VIGNDATVTVAGQSGNFQLNVMLPVIAYNLIQSIELLGSGARLLADKALKEFEVAEDT
                     ISERLARNPILVTALNARVGYDAGAAIAKRAYAEGRSVLEVALEMTTLSREELERLLD
                     PAALAGPPRG"
     gene            complement(2413..2817)
                     /locus_tag="AC16UZ_14115"
     CDS             complement(2413..2817)
                     /locus_tag="AC16UZ_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0015671 - oxygen transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HIR6247869.1"
                     /translation="MSDLVLESLDAAAETGQDVTEAVNRAFRDREPQAAGLMDHMDEH
                     MLGRMMQEVLMVLMSENPEDQLEYLNFEVGSHLGYGVTAPMYGALFAAVRDCVRDLVG
                     EAWSPATETAWKQRIRSLQEIVETTAKAPADA"
     gene            complement(2810..>3232)
                     /locus_tag="AC16UZ_14120"
     CDS             complement(2810..>3232)
                     /locus_tag="AC16UZ_14120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6247870.1"
                     /translation="ARLGEARTAYLGFCMAALSCLGYAFASQGWMIFPSIAVGAFMGL
                     VMPSLRSIMSQMVPEDGQGELQGAIASLMGLTAIFAPLIMTQVFRYFSAQEAGVYFPG
                     ASFLLAGLLMLLGALVVMAVLRHPSAPPGQPSSETGDV"
BASE COUNT          570 a         1128 c         1043 g          491 t
ORIGIN      
        1 cggcccgcgg ctcgcgcctg ccaggccggc aatgctcata cttcctcgcc gagaccccgc
       61 ttggccacca gggcttcgcg atcgagctcg taaggcgcac cccgggcgac gatggcgtcg
      121 accctgccca gaaccacctc gtcccagccg gcgtggggga ggatgtagaa ccgctcctcc
      181 cggatggagt tcagcacctt ttccgccacc acctccggcg gcataccctg cttgagggta
      241 tctcccagcg gcagcccatg cccttcgggg tttttcgcgc tagtcatggc cgtgggccgg
      301 ttgcgctcgg cttcggcgat cttcgtatcc acccaccccg ggcagaggac ggacgcgccg
      361 agggccgaac cggccgcgcg gagatcccgg tgaagcgcct cgctgatgac cagcaccccg
      421 tgcttgctga cgccgtaggt gtcgcccccg ggcatgaggg cggcgaccga cgccgtgttg
      481 acgatgtgcc cgggctcgcc gtgggccagc atgtgcggca cgaaggtctg gagcccgtaa
      541 agaaccccgt agaagttgac gcccatgacc cattcccagt cggcgtccgg aatctcccag
      601 acgggcacgt gctcgccgcc gacgacgacg cccgcgttgt tgcagagcac gtgaacgttc
      661 ccgaactcgg caacggcgcg ctcgagaagc gcttcgatag cgtcgcgccg catggtgtcc
      721 gtgaccacgc cgagggcccg gtgctgacgg gcttcgagat gctcgaccgc agcgcgcagc
      781 gcattctctt cgatatccgc gagcaccacg ttcatgcgct cctcggcaaa ccgctgcgcc
      841 atggcaagcc cgatgccgct ggcacccccg gtgatgaccg ccgtgcggcc cgcaaagtct
      901 ttcatgtccg ctccccgaac gagcgttcta agataacagg attgcgcggc gcctctacac
      961 cgatcggtta agatcctgct gctgccgcgc gatcaggaga aacccatgag cggaaagacg
     1021 cgaaccgaga aagacagcat gggagcgctc gaggtgcccg ccgatgcctt gtggtcggca
     1081 cagacccagc gcgccgtgga gaacttcccc atcagcggga tcaggatgcc cggcgccttc
     1141 atccgttcgc tgggcctgat caaggccgcc gcggcacacg tcaacgcgga tctcggcgtg
     1201 cttccggcgg agcttgcgct gcccatcgtc gaggccgccg agcgggtcgc cgccggcagc
     1261 gtggacgatc acttccccgt ggacgtcttc cagaccggat cgggcaccag cagcaacatg
     1321 aacgccaacg aggtcatcgc ccgccttgca tccgatgcat cggggctgga ggtgcatccc
     1381 aacgatcacg tgaatgccgg ccagagcagc aacgacgtga tccccacggc catccacgtc
     1441 agcgcagtgc tcgccacgcg ggaagacctg ttgccggcat tggcgcatct ggccggcgtc
     1501 ctgagagagc gggcggccgg gttgcagggc gtggtgaaga cgggccgcac gcacctcatg
     1561 gacgccatgc ccatccggtt gggccaggag gttggggcgt gggccactca ggtggagcag
     1621 gacatcgccc gcattcgcga caccctgcca cgctgtgagc gcctcgccca gggcggcacc
     1681 gcggtgggaa ccggcgtcaa cgcgcatccg gagttcgccg tgcggttcgc ccgtcgcctc
     1741 agcgacgtca ccggcgttcc cttcctcccc agcgaagatc tgcccgcaag cctcgccagc
     1801 caggacgctg ccgttgagct ctccgggcag ctgaaggtca ccgcggtgag cctcatgaag
     1861 atcgccaacg acctgcgctg gatgaacagc gggccgctgg ccggcatcgc ggagatcatg
     1921 ctgccggcgc ttcaacccgg cagcagcatc atgccgggca aggtcaatcc ggtaatcccc
     1981 gaagccatcg ccatggtctg cgcccaggtg atcggcaacg acgccacggt gacggtggcc
     2041 ggccagtcgg ggaacttcca gctgaacgta atgctgcccg tcatcgcgta caacctgatt
     2101 cagagcatcg agctgctcgg cagtggcgcc cggctgctgg ccgacaaggc gctcaaggaa
     2161 ttcgaggtgg cggaggacac gatcagcgaa cggctcgcgc gcaaccccat tctggtgacc
     2221 gcgctgaacg cgcgggtggg ctacgatgcc ggggcggcca tcgccaaacg ggcgtacgcc
     2281 gagggccgct cggtgctcga ggtcgctctg gagatgacga cgctcagccg cgaggagctc
     2341 gagcgcctgc tggaccccgc tgcgctcgcc ggaccgccgc gaggctgaac cgcccgtccg
     2401 gcggcgcctc gctcacgcgt cggcgggagc tttcgcggtg gtctcgacga tctcctgaag
     2461 gcttcggatg cgctgtttcc aggccgtttc cgtggccggg ctccaggcct cgccgaccag
     2521 gtctcgcacg cagtcgcgca ccgcggcaaa cagggcgccg tacatgggcg cggtaacgcc
     2581 gtaaccaagg tgcgagccga cctcgaagtt cagatactcc agctgatcct cggggttctc
     2641 ggacatcagc accatgagca cctcctgcat catgcgacca agcatgtgct cgtccatgtg
     2701 atccatgagg cccgccgctt ggggttcccg gtcgcggaag gcccggttga cggcctcggt
     2761 cacgtcctga ccggtttcgg ccgcggcgtc caaggattcc agaaccagat cagacatcgc
     2821 cagtctccga cgaaggttgg ccgggaggcg ccgaaggatg cctgagcacg gccatcacca
     2881 ccagcgcgcc aaggagcatc agcagtcccg ccagaagaaa cgaagcgccg gggaagtaaa
     2941 cgccggcttc ctgagccgaa aaatatctga agacctgggt catgatcagc ggcgcgaaga
     3001 tggccgtcag ccccatgaga ctggcaatcg cgccctgcag ctccccctgc ccgtcctccg
     3061 gcaccatctg cgacatgatg ctgcgcagag acggcatgac caggcccatg aacgcaccca
     3121 cggcaatgct ggggaagatc atccatccct ggctcgcaaa cgcatagccc aggcagctca
     3181 gcgcagccat gcagaatccc aggtacgccg tgcgcgcctc gcccagacgg gc
//
LOCUS       DBNHOX010000641         2532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_244918, whole genome shotgun sequence.
ACCESSION   DBNHOX010000641 DBNHOX010000000
VERSION     DBNHOX010000641.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_244918"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="AC16UZ_14125"
     CDS             complement(<1..525)
                     /locus_tag="AC16UZ_14125"
                     /inference="COORDINATES: protein motif:HMM:NF023169.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HIR6247871.1"
                     /translation="MKSKLLVSAFLLLCYAPPSSAGKLDRVRLHEAPDHTRVVFDVAA
                     PVEFSYFTLRNPDRVVIDLKDTTAGNDFDPSRTGTKSSRVKNIRAAPRGRDYRVVLDV
                     SAPLEPKAFALEPVAAYGHRLVVDLFGAAPKPAAPAAPKADDRRDVIVAIDAGHGGED
                     PGALGPGKVREKDVV"
     gene            complement(503..922)
                     /gene="tsaE"
                     /locus_tag="AC16UZ_14130"
     CDS             complement(503..922)
                     /gene="tsaE"
                     /locus_tag="AC16UZ_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR6247872.1"
                     /translation="MESVFLPDAEATEAFGRDLAVRLEPGDLVLLHGDLGAGKTTLVR
                     GILAGLGHSGSVKSPTYTLLEPYELTSCVVLHFDFYRIGDSRELEFIGFDELMDADAI
                     KLVEWPERAADRLPPPDLEITLRVEDEGRRIEVEAAR"
     gene            complement(934..2433)
                     /locus_tag="AC16UZ_14135"
     CDS             complement(934..2433)
                     /locus_tag="AC16UZ_14135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR6247873.1"
                     /translation="MEDRALNRVYTGRECRELDRIAIEEFGIPGFELMMRAGRAAFSE
                     LLARWPDASCLTILCGKGNNAGDGYVIGGLAREIGMAVQLVQLGDAADLAGDAARARD
                     WAVERGVLIEPGPERALRGDVLVDALLGTGIKGELKPAYREQVEAVNGSGISVLAVDV
                     PTGVDADTGAACEPSVRADVTVTFIARKLGLVTGPGASFCGNLVLAGLGVPDQVYDSV
                     AGTALLLHENLEALPARDANAYKQALGHLVVVGGDHTMGGAALMAGEAALRVGAGLVS
                     VVTRPAHRPAILARRPELMVVDADDDQSRREVLARATTIVLGPGLGRSAWGERLSREA
                     LALGKPAVIDADGLYWLATGALEPTAPVIITPHSGEAAMLLDSTSAAIQADRVTAVEH
                     LAEKVAGVAVLKGAGTLIAEHLGSTSRLAGVCGHGNPGMASAGMGDVLSGIIGGLLAQ
                     HDVLLSAAVRGVCLHSAAADVVARQVGQRSMLATDLLPEVVSMLAAAEQ"
BASE COUNT          442 a          894 c          797 g          399 t
ORIGIN      
        1 gaccacgtcc ttctcacgca ccttccctgg cccgagcgcg cccggatcct cccccccgtg
       61 accggcatcg atggcgacga tcacgtcacg ccggtcatcg gccttcggcg cagccggcgc
      121 ggcgggtttc ggtgcggcgc cgaaaagatc gaccaccaga cggtggccgt acgcggcaac
      181 cggttcgagc gcgaaagcct tcggctcgag gggggcggaa acgtccagca ccacccgata
      241 gtcgcgtccc ctcggcgctg cgcgaatgtt cttcacacgg ctcgatttag tcccggtccg
      301 cgaaggatcg aagtcgtttc ccgcggtggt gtccttgaga tcgatgacca cccggtccgg
      361 gttgcgcaac gtgaaataag agaactcgac aggtgcggcc acgtcgaaaa ccacgcgggt
      421 gtgatcgggg gcctcgtgca ggcgcactcg gtccagcttg ccagccgagg atggcggcgc
      481 gtaacaaagg agcagaaatg cgctaacgag cagcttcgac ttcaatacga cgaccttcgt
      541 cttccacgcg cagggttatc tcgagatcag gcggaggcag gcgatcggcc gctcgctccg
      601 gccactccac gagcttgatg gcatctgcgt ccatcagctc gtcaaatccg atgaactcga
      661 gttcccggct gtcccctatt cgataaaaat cgaaatgcag gacgacacac gacgtcaatt
      721 cataaggctc gagcagagta tacgtgggac tcttgaccga accggaatga cctaaacccg
      781 cgagtatgcc cctgaccagg gttgtctttc cagcaccgag gtccccatgc agcagcacca
      841 gatcgccggg ctcgagccgg accgccaggt cgcgcccgaa cgcctccgtg gcttccgcgt
      901 ccggcagaaa cacggattcc accgcgaccg gtctcactgc tcggcggccg ccagcatgga
      961 caccacttcc ggcagaagat ccgttgctag catgctgcgt tgaccgactt gtcttgccac
     1021 cacatcggcg gcggcgctgt gcagacatac gcccctgacg gcagcactga ggagcacatc
     1081 gtgctgggcg agcagcccgc cgatgatccc gctcagcacg tcgcccatgc ctgcgctggc
     1141 catcccggga ttgccgtgac cgcaaacgcc tgccaggcgg ctcgtcgagc cgagatgttc
     1201 ggcaataagc gttccggcgc ccttgagcac ggcgacgccg gcaaccttct ccgccagatg
     1261 ctcgaccgcc gtcacccggt ctgcctggat cgcggcagag gtgctgtcga gcagcatcgc
     1321 cgcttctccc gaatgcgggg tgatgatgac gggcgccgtc ggctccagcg cgcctgtcgc
     1381 gagccagtaa agaccatcag catcgatcac ggccggtttg ccgagggcca gcgcttcccg
     1441 cgacagccgc tcgccccacg cgcttcggcc gaggccgggt cccagcacga tggtcgttgc
     1501 ccgggcgagg acctcgcgcc tgctctggtc gtcgtcggca tccacgacca tcagctccgg
     1561 gcgccgggcg agtatggccg gccggtgggc tgggcgggtc acgacgctca ccagcccggc
     1621 gccaacccgc agcgcggctt ccccggccat cagcgcggcg ccgcccatgg tgtggtcgcc
     1681 gccaacgacc acgaggtggc cgagcgcctg cttgtaggca ttggcatccc gagcgggcag
     1741 cgcttccagg ttttcgtgca gcaggagggc agtgccggct accgagtcgt acacctgatc
     1801 aggcacgccg aggccggcaa gcaccaggtt gccgcagaac gatgcacccg gcccggtgac
     1861 cagccccagc ttgcgagcga tgaaggtcac cgtgacgtcg gccctgaccg aaggctcaca
     1921 tgccgcgccg gtgtccgcat ccacgccggt aggtacgtcc accgcgagca cgctgatccc
     1981 cgagccgttg acggcctcga cctgctcgcg ataggccggc ttcagctctc ccttgatgcc
     2041 cgtgcccagc agagcgtcga ccaggacatc gccacgcagc gctctttccg gtcccggctc
     2101 gatgaggacg ccacgctcga cggcccagtc tctcgcccgc gcggcgtcgc ccgcgaggtc
     2161 tgcggcatcg cccagctgga ccagctgcac ggccatgccg atttcccggg cgagcccgcc
     2221 gatgacatac ccgtcccccg cgttgttgcc cttgccgcac aggatcgtga ggcacgaggc
     2281 atcgggccag cgggccagca gctcggagaa ggcggcgcgc ccggcacgca tcatgagctc
     2341 gaagccggga atcccgaact cctcgatggc gatgcggtcg agctcgcggc actcgcggcc
     2401 ggtgtaaacg cgattcaggg ctcgatcctc cacatcaccg tcgatgaaga ccaggcatta
     2461 tactgggtgg cttccccgcc gatacgtgga ttcagagcgg atggccgacc tagcagcgct
     2521 gcgcaaggac at
//
LOCUS       DBNHOX010000642         3456 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1016218, whole genome shotgun sequence.
ACCESSION   DBNHOX010000642 DBNHOX010000000
VERSION     DBNHOX010000642.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1016218"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..172
                     /locus_tag="AC16UZ_14140"
     CDS             11..172
                     /locus_tag="AC16UZ_14140"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6247874.1"
                     /translation="MFHPLLVHGSGRNRSDHFRRAISSHYASAACESPVRDWRIGKQV
                     RHIPAGKEG"
     gene            complement(169..1428)
                     /locus_tag="AC16UZ_14145"
     CDS             complement(169..1428)
                     /locus_tag="AC16UZ_14145"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6247875.1"
                     /translation="MNEQEIRTLRQLMEYEVGRNSPPDGFPDLPDLPGGRYTDQRFYD
                     LEQEHIWRKSWLLAGHIDEIPEPGCFRLWETAGQPVVIVHADGGAVNAFYNTCSHRGA
                     PVVTDASGKRDRLVCRYHGWTYSHDGELLAVRDPQDFGDLDFSCRGLRKIRCERFGNL
                     IFVNFDDAAPTLLEWLGPIADEWKEFQFDNCRLAARHMWPLNCNWKIAMEANTEVYHV
                     RSIHAQTVSPMLDDRRNVNTFYPNGHGRMVAPGRRNENGELVRQRPPEMAEIETVGEI
                     GRTCTQSYGIFPNWVSPLSHVALPPLLFWPNGINKCFLETWTLAPDWGDGPKPDVWTV
                     NDGESLSPVLIEDTEFGQWIQKSVESYGFRGVPLSYQEARIYHWNQFADRMIGIENIP
                     EELRVAQVIGPEWVYPNDPRVALIAAG"
     gene            complement(1449..1868)
                     /locus_tag="AC16UZ_14150"
     CDS             complement(1449..1868)
                     /locus_tag="AC16UZ_14150"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6247876.1"
                     /translation="MSDQYVADRIALQDVMLKYAAGVDNRDFDLYRSCFADDVEVLGF
                     GDETVNGGDAWLAYVKNALERFGPTQHMLGPQLATVDGDDAHCRTDVQALHYLKGEES
                     TTLTLWATYETDMRRIDGTWKIVRHRLVSRGTRIQSD"
     gene            2034..2858
                     /locus_tag="AC16UZ_14155"
     CDS             2034..2858
                     /locus_tag="AC16UZ_14155"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6247877.1"
                     /translation="MGSLQGKIAVVTGASRGIGKGIALALGEQGCTVYVTGRTRGDGE
                     RTIDTTARLVTEAGGEGRAIQCDHGDDEQIAALFQQVKEEAGHIDILVNNVYKIPDPP
                     AWGGGFWDHPIQVWDDQVGIGLRAHYVASWHAAPLIFAAGAGGAILNVSSPGGQSYHF
                     SSSYGAGKAGLDRLTADMAIELEPKGVAAVVLYPGSVATEFIQDHAEAQGMDLSESQT
                     PLYVGRCAVAMLAADDLMSRSGSIQWVEDLGEEFDILDEHGKRPPGYAQRPGTQAS"
     gene            complement(2905..>3456)
                     /locus_tag="AC16UZ_14160"
     CDS             complement(2905..>3456)
                     /locus_tag="AC16UZ_14160"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6247878.1"
                     /translation="VTSAEQLETLVVAAQELGGLDIWVSNAGGLPDATPRYLTRTPED
                     RWDAQIDLNLKAVWQGAVVAAQNMDRGVIINISSRSAQGPSFKNGPYGASKAAVNSLT
                     QTLSLELAPNIRVNAVAPGPIPTENFNESTNFPDDKPIEQIIGVPLGRLGTPEDIGNA
                     VVFMASPAAAWITGQCLYVTGGL"
BASE COUNT          589 a         1156 c         1089 g          622 t
ORIGIN      
        1 cgacaccctg ctgttccatc cgctgctggt gcacggttcc ggccggaacc ggtcggacca
       61 cttccgacgg gccatctctt cacactacgc ttccgcggcc tgcgaatcgc ccgtcagaga
      121 ctggcgcatc ggcaagcagg tgcgccacat cccggccgga aaagagggtt aacctgccgc
      181 gatcagcgcc acccgcgggt cgttcggata gacccattcg gggccgatga cctgggccac
      241 ccgcaactcc tccgggatgt tctcgatgcc gatcatccgg tccgcgaact ggttccagtg
      301 atagatgcgc gcctcctgat agctcagcgg caccccgcgg aacccgtacg actccaccga
      361 tttctgaatc cattgcccga actcggtgtc ctcgatgagc acaggactca agctctcgcc
      421 gtcgttgacg gtccagacat cgggcttcgg cccgtcaccc cagtccggcg ccagcgtcca
      481 cgtctccaga aagcacttgt tgataccgtt cggccagaac agcagcggcg gcagggccac
      541 gtggctgagc ggcgaaaccc agttggggaa gatgccgtat gactgggtgc acgtcctgcc
      601 gatctccccc accgtttcga tctcggccat ctccggcggg cgttgcctca caagctcgcc
      661 gttttcgttc ctgcgaccgg gcgcgaccat ccggccgtgc ccgttggggt agaacgtgtt
      721 gacgttgcga cgatcgtcga gcatcggcga gaccgtctgc gcatggatac tgcgaacgtg
      781 gtagacctcg gtgttcgctt ccatcgcgat cttccagttg cagttgagcg gccacatgtg
      841 gcgagcagcc aggcggcagt tgtcgaactg gaactccttc cactcgtcgg cgatggggcc
      901 gagccactcg agcaacgtag gggcagcgtc gtcgaaatta acgaagatga gattgccgaa
      961 gcgctcgcag cggattttcc gcaggccgcg acaggaaaaa tccagatcgc cgaagtcctg
     1021 aggatcccgg acggccagaa gctcgccgtc gtgactgtag gtccagccgt ggtaacggca
     1081 gacgagcctg tcgcgctttc ccgacgcatc ggtcaccacc ggcgcgcccc ggtggctgca
     1141 ggtgttgtag aacgcgttga ccgcgccgcc atcggcatga acgatgacca ccggctgccc
     1201 ggcggtctcc cacaggcgga agcacccggg ctcggggatc tcgtcgatgt ggccggccag
     1261 cagccaggac ttgcgccaga tgtgctcctg ctccagatcg tagaagcgct gatccgtgta
     1321 gcgccctcct ggaaggtccg gcaggtccgg gaacccgtcg ggcggcgaat tgcggccgac
     1381 ctcgtactcc atcagctgcc tgagcgtgcg gatttcctgc tcgttcatag gatcgattcc
     1441 tcaaccgttc agtctgattg gatccgtgtc ccccgcgaca ccagcctgtg acggacgatt
     1501 ttccacgtgc catcgattcg gcgcatgtcc gtttcgtaag tcgcccacag cgtcagcgtg
     1561 gtgctctcct ctcccttcag ataatgcagc gcctgcacgt cggtccggca gtgcgcgtcg
     1621 tcgccgtcca cggtggccag ctgcgggccc agcatgtgct gagtgggccc gaaacgttcc
     1681 agggcgttct tgacgtaagc cagccacgca tcgccgccgt tgacggtctc gtcgccgaat
     1741 cccagcactt ccacatcgtc ggcgaagcac gaccggtaaa ggtcgaaatc gcggttatcc
     1801 accccggcgg cgtatttgag catgacgtcc tgcagggcga tgcggtcagc cacgtattga
     1861 tcactcatcc ctctctccct cgagccaatg tccttcaaat ctagcccata aaggaagagc
     1921 gcaccagccg ggcgggccgc cggcgagcac tggcaatctc gccgcgcccc ggtgcctggg
     1981 tgggcaggct tctggtatcc tgcgcgcccc gctcactgca ttgggaaaca gatatgggtt
     2041 cgttacaagg aaaaatcgcc gtggtcaccg gggccagccg gggcatcggc aagggtatcg
     2101 cccttgctct gggcgagcag ggctgcaccg tctacgtgac cggccgcaca cgcggcgacg
     2161 gtgagcggac catcgacacc accgcccggc tggtgaccga ggcagggggc gagggccgag
     2221 ccattcagtg cgaccatggg gacgacgagc agatcgccgc cctgttccag caggtcaagg
     2281 aagaagccgg gcacatcgac attctcgtca acaacgtcta caagatcccc gatccgcccg
     2341 cctggggcgg cggtttctgg gatcacccga tacaggtctg ggacgatcag gtgggcattg
     2401 gcctgcgcgc ccactacgtg gccagctggc atgccgcccc tctgattttc gcggcgggtg
     2461 ctggcggcgc cattctcaac gtgtcgtcgc ccggcgggca gagctatcac ttctcttctt
     2521 cctacggcgc gggcaaagcc ggcctggacc gactcaccgc cgacatggcc atcgagctcg
     2581 agcccaaagg ggtggccgcg gtggtgctgt acccgggctc cgtcgccacg gagttcatcc
     2641 aggaccacgc ggaagcccag gggatggatc tttccgaatc ccagacgccc ctctacgtcg
     2701 ggcggtgcgc cgttgcgatg ctggccgccg acgacctcat gtcacgcagc gggtcgatcc
     2761 agtgggtgga agacctcggc gaggagttcg acatcctcga cgagcacggc aagcgcccgc
     2821 ccggctacgc tcagcggccg ggcacccagg cctcgtaagg cggggcccgg tcctctcctt
     2881 gctccgggga agacgggcga tcgcctaaag cccgccggtc acgtagaggc actgccccgt
     2941 tatccaggcc gccgccggcg aggccatgaa cacgacggca ttgccgatgt cctcgggcgt
     3001 gccgagccgt cccaggggca ccccgatgat ctgctcgatg ggtttgtcgt ccgggaagtt
     3061 cgtggattcg ttgaagttct ccgtcgggat cggtccgggc gctacggcgt tcacgcggat
     3121 gttcggcgcc agctccagcg aaagcgtctg ggtaaggctg ttcacggccg ccttggaggc
     3181 accgtaaggc ccgttcttga aagaagggcc ctgagcggat cgtgaggata tgttgatgat
     3241 gacaccgcga tccatgttct gcgccgcgac gacggcaccc tgccacacgg ctttgagatt
     3301 gaggtcgatc tgcgcatccc agcggtcctc cggcgtccga gtcaggtatc gcggagtcgc
     3361 gtctggcaga ccgcccgcat tgctgaccca gatgtcgagc ccgcccagct cctgtgccgc
     3421 caccaccagc gtctcgagct gctccgcgct ggtgac
//
LOCUS       DBNHOX010000643         4473 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_181177, whole genome shotgun sequence.
ACCESSION   DBNHOX010000643 DBNHOX010000000
VERSION     DBNHOX010000643.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_181177"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="AC16UZ_14165"
     CDS             <1..788
                     /locus_tag="AC16UZ_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6247879.1"
                     /translation="LNYVIHRAGHQTSRIAVGDNLFARPFAADLMRLNKSFVVERKVS
                     GTRAVYAALSRTSHYIRQSLEEGESVWIAQREGRSKDGFDRTDPALLKMLGLAYRKEA
                     GSFGDLVTRIRIVPVAVSYELDPCDLRKAHELYLTEREGSYIKPPDEDFQSIVEGMMG
                     FKGRVHLHFGSPVTGYFETAEALALEIDRAIVAGLKIFPTQAQAAARLGQASCHVQLA
                     ELPAVTAAFEQRLAGCPDAEKPYLLAAYANVLRNRRELSLPSA"
     gene            complement(769..1755)
                     /locus_tag="AC16UZ_14170"
     CDS             complement(769..1755)
                     /locus_tag="AC16UZ_14170"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR6247880.1"
                     /translation="MSESSTSTPYAGLTPDLILDALEATGFAPTGSLLELNSYENRVY
                     QLEVEDGTFVVTKFYRPERWSDAQILEEHAFTAELIEHELPAVGPLRRDGTTLFEHSG
                     FRFAVFPRQGGHPPNIEVEEDLKVLSRTLARLHAIGRIRPFETRPTLSCERMGRQSRE
                     FLLETNLVPLELQPAYASTTEHLLERLEPLFDTEHQWRRIHGDCHLGNLLWRGEVPHF
                     VDFDDCVNGPPIQDLWMLLSGEHHEREAQLSMILEAYDQFASFDLSSLRLIEPLRTLR
                     IMHHAAWIGRRWSDPAFPRAFPWFNTEKYWSEHILSLREQMAALDEPTLTVS"
     gene            complement(1778..2473)
                     /locus_tag="AC16UZ_14175"
     CDS             complement(1778..2473)
                     /locus_tag="AC16UZ_14175"
                     /inference="COORDINATES: protein motif:HMM:NF039185.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR6247881.1"
                     /translation="MIGRALRALFPPICLLCDQSCAAEADVDLCSRCLPVLPWNPQPC
                     PRCALPRENGTGRCQPCEDDPLPVARTVAPLLHAGYPRHWVHRLKFHEGMVEGRLLAH
                     LLAGAVRESYGRTDLPDMLVPVPTAPGRLAWRGHNQAATIAAFLGRRLSLPLARRGAR
                     RARSSRPQRLLSRAARLKATAGIFESRQWRGETIAVVDDVMTTGATVAELARTLTAAG
                     AGAVHVWSATRAV"
     gene            2680..4446
                     /locus_tag="AC16UZ_14180"
     CDS             2680..4446
                     /locus_tag="AC16UZ_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6247882.1"
                     /translation="MPASLKDVRFLIHDVFDFAGHYARLELDEPPGRDLIDAIVEEAA
                     RFADGELAPLNRVGDESGCRLEDGSVSTPAGFKEAYAKYVEGCWAGLSGSAAYGGQGL
                     PDSMSFFLEDLMCCANMAWTMYPGLSRGVVGALEEHGSAELKDAFLANLLSGEWTGTM
                     CLTEPHAGSDVGLARTRAEASADGTYRISGTKIFISAGEHDMAENIVHLVLARIPDAP
                     PGTKGISMFVVPKFDLDGTRNGVSCGSIEEKMGIHGNATCVLNFDGARGYLVGEPNHG
                     MRYMFTMMNGARLVVGLQGASQAHAACLLSLEYAQDRLQMRSLTGPKAPERPADPIIV
                     HPDVRRMLLFQRAIAEGGRALVYYAAQIIDRFRDGSPDERASAGELLDFLTPVVKGIL
                     TELGFESVNQAVQIYGGHGFIRETGVEQYVRDTRITTIYEGTTQIQALDLVGRKILQN
                     QGKGLVSFIGEMQAVAASLEDPLPRLAAPLKALADEWGELTLQLSATAMQDLDEVGAA
                     AVDYLYYSGYAALAFCWALMARSADEAIRAGSTDPFHAGKIAVADFFFSRVLPRAKAH
                     QAAIEAGKGTLMDVDEGALVAL"
BASE COUNT          751 a         1422 c         1524 g          776 t
ORIGIN      
        1 tcctcaacta cgtgattcat cgagcggggc accagacgtc gcgcatcgcg gtcggggaca
       61 acctgttcgc gcggccgttc gccgccgacc tgatgcgcct caacaaaagc ttcgtggtcg
      121 agcgcaaagt cagcggtacc cgagccgtct acgccgcgct ttcgcgaacg tcccactaca
      181 tccgccagtc gctcgaggag ggcgagtcgg tgtggatcgc gcaacgcgag ggtcgatcga
      241 aggacggttt cgatcgcacc gatccggccc tgctgaagat gctgggtctc gcctaccgca
      301 aggaagcggg gagcttcggg gatctcgtca cgcgaatccg catcgtcccg gtagccgtct
      361 cctacgagct cgatccctgc gatctgcgca aagcccacga gctttacctg acggaaaggg
      421 aaggctccta catcaagccg ccggacgagg atttccagag catcgtggag ggcatgatgg
      481 gcttcaaggg tcgcgtgcat ctgcacttcg gctcgcccgt gaccggctac ttcgaaactg
      541 cggaagccct cgccctcgag atcgatcgcg ccatcgtcgc gggcctgaag atctttccca
      601 cccaggcgca ggcggcggcc cggctcggcc aggccagctg ccatgtgcag ctggccgagc
      661 ttcctgccgt gaccgccgcc ttcgagcagc gtcttgccgg gtgccccgac gccgagaagc
      721 cctatctcct ggcggcgtac gccaacgtcc tgcgcaatcg ccgggagctc agcttaccgt
      781 cagcgtaggc tcgtcgagag cggccatctg ctctcgcagg ctgagaatgt gttccgacca
      841 gtacttctcc gtgttgaacc acgggaacgc acggggaaag gccggatcgc tccagcggcg
      901 gccgatccag gcagcgtggt gcatgattcg cagcgtgcgc aggggttcga tcagcctgag
      961 gctcgacagg tcgaagctcg caaactgatc gtaagcctcc aggatcatgc tcagctgggc
     1021 ttcgcgctcg tggtgctcgc cggacagcag catccacagg tcctgtatcg gcggaccgtt
     1081 cacgcagtcg tcgaaatcga cgaagtgtgg aacctcgccg cgccacagca ggttgcccag
     1141 gtggcagtcg ccgtggatgc ggcgccattg gtgctcggta tcgaacagcg gctccagccg
     1201 ctcgagcaga tgctcggtgg tgctggcgta ggccggctga agctccaggg gaacgaggtt
     1261 cgtttccagc agaaactcgc ggctctgcct gcccattcgc tcgcagctga gcgtggggcg
     1321 tgtctcgaac gggcggatcc gaccgatggc atgaagccgg gccagcgtgc gggagagaac
     1381 cttcagatct tcctcgacct ctatgttggg agggtgcccg ccctgcctcg ggaacacggc
     1441 gaaacggaat ccggagtgct cgaacagggt ggtgccgtct ctccgcagcg gccccaccgc
     1501 gggcagctca tgctcgatga gctctgcggt gaacgcgtgc tcttcgagga tctgcgcgtc
     1561 gctccagcgc tcgggacggt agaacttggt cacgacgaac gtcccgtcct caacctccag
     1621 ctgatagacg cggttttcgt agctgttcag ctcgagcagg cttccggttg gggcgaaccc
     1681 ggtggcctcc aacgcgtcga gtatcaggtc cggggtcagg ccggcatagg gtgtgctggt
     1741 ggacgactcg ctcatgagag ccagtctacc agggaactca gacggcgcgg gtggccgacc
     1801 acacgtggac tgcgcccgca cccgccgccg tcagcgtgcg tgcgagctcg gcgactgtag
     1861 ccccggtggt catgacgtcg tcgaccacgg cgatcgtctc gccccgccac tgacgtgatt
     1921 cgaagatgcc ggcagtggcc ttcaggcgtg cggcgcggga cagaagtctc tggggccggg
     1981 agctgcgggc gcgcctggct ccgcgccggg ccagcggcag gctcaggcgg cggcccagga
     2041 aagcggcgat ggtggctgcc tgattgtgac cccgccaggc cagccgtccc ggagccgtgg
     2101 gaacgggcac cagcatgtcc ggaaggtcgg tcctgccgta gctttcacgg accgccccgg
     2161 cgagcaggtg cgccagcagc cggccctcga ccatgccctc gtgaaacttg agccggtgga
     2221 cccagtggcg cgggtagccg gcgtggagca gcggcgccac ggtgcgggcc accggcagag
     2281 ggtcgtcttc gcagggttgg catcgcccgg tgccgttctc ccgcggaagc gcgcagcggg
     2341 gacagggctg cgggttccag ggcaggacgg gcaggcaccg gctgcacagg tctacgtctg
     2401 cctcggctgc gcagctctga tcgcagagca ggcagatggg cgggaacagg gctcgcaacg
     2461 cgcgaccgat catcggttga tcctgcccgt tggacgtttg gcacgacagt agcgaatgtc
     2521 ctcgtatggc gtgcgaagcc cgtcacgatt cacccgctcc tccgggcggg agcaggccgc
     2581 gattttgcgc atcagcgcgg ggcgaacgcg atctgtggag ggggtcattg acgggccgcg
     2641 aagatttgct accgtgccct ccgcattgga ggatccaaga tgccagcatc gctcaaagac
     2701 gtgcggtttt tgattcacga cgtgttcgat ttcgccgggc actacgcgcg gctcgagctc
     2761 gacgaaccac cgggccgcga tctcatcgac gccatcgtcg aggaagctgc gcggtttgcc
     2821 gatggcgagc tggcgccgct caaccgcgtt ggggacgaat ccggctgccg actggaagac
     2881 gggtcggtga gcacgccggc cgggttcaag gaggcctacg ccaagtacgt cgaaggatgc
     2941 tgggccggtc tcagcggttc cgctgcctac ggtggccagg gcctgccgga cagcatgagc
     3001 tttttcctgg aggacctgat gtgctgcgcg aacatggcct ggaccatgta cccgggcctt
     3061 tcgcgggggg tcgtcggcgc tttagaggag cacgggagcg cggagctcaa ggacgcgttt
     3121 ctcgccaatc tcctctcggg tgagtggacg gggaccatgt gtctgaccga gccccacgcc
     3181 gggtcggacg tgggcctggc cagaacccgg gcggaggcct ctgcagacgg aacgtaccgg
     3241 atctcgggga ccaagatctt catctctgcc ggcgagcacg acatggcgga gaacatcgtc
     3301 cacctggttc ttgcccgcat tccggacgcg cctccgggaa ccaagggcat atccatgttc
     3361 gtcgtgccca agttcgatct cgacggcacg cgcaatggcg tgtcgtgtgg ctccatcgag
     3421 gagaagatgg gcatccacgg caacgccacc tgcgttctca acttcgacgg cgccagggga
     3481 tatctggtcg gcgagccgaa ccacggcatg cgttacatgt tcacgatgat gaacggcgcg
     3541 cggctggtgg tgggtctgca gggcgccagc caggcccacg cggcctgcct gctgtcgttg
     3601 gaatacgccc aggatcgcct gcagatgcgc tccctcacgg ggcccaaggc gccggagcgg
     3661 ccggcggatc ccatcatcgt gcacccggac gtgcggcgaa tgctgctctt ccagcgcgcc
     3721 atcgcggaag ggggacgggc gctcgtctac tacgccgcac agatcatcga tcgtttccgg
     3781 gacgggtccc cggatgagcg ggcttcggcc ggcgagctgc tcgattttct gacccccgtg
     3841 gtcaagggta tcctgacgga gcttggcttc gagtcggtca atcaagccgt gcagatttac
     3901 ggtggccacg gcttcatccg ggagaccggt gtcgagcagt acgtgcgcga caccagaatc
     3961 accaccatct acgagggcac cacgcagata caggccctcg atctcgtggg tcggaaaatt
     4021 ctgcagaacc agggcaaggg gctcgtcagc ttcatcggcg agatgcaggc cgttgccgcc
     4081 tccctggagg atccgctgcc ccgtctggcc gctcctctca aagcgcttgc cgacgagtgg
     4141 ggcgagctca cgctgcagct cagcgcgacg gccatgcagg atctggatga ggtaggtgct
     4201 gcggccgtgg actacctcta ctacagcggg tacgcggcgc tcgctttctg ctgggctctc
     4261 atggcgcgca gcgcggacga ggcgattcga gcggggtcga cggatccctt tcatgcaggc
     4321 aagattgcgg tcgccgactt tttcttcagt cgcgttctgc cgcgcgcgaa ggcacaccag
     4381 gctgccatcg aagcgggcaa agggactctc atggacgtcg acgaaggtgc gctggtcgcg
     4441 ctctgaacac cgtgcgcttg aagtcatgat tgc
//
LOCUS       DBNHOX010000644         1556 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1079968, whole genome shotgun sequence.
ACCESSION   DBNHOX010000644 DBNHOX010000000
VERSION     DBNHOX010000644.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1079968"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="AC16UZ_14185"
     CDS             <1..509
                     /locus_tag="AC16UZ_14185"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6247883.1"
                     /translation="MLEQGRGGEIVNVATDHMVTCGSPFEICPKLETCPWADSPRPTG
                     GGDVMDVYDASKWALNGFLFAWAKALRPHGIRVNGMCMGATDSWMIRDFYGFPQTPGE
                     ETEEQMEEVSTWMAKGDTAQVVMNLLKEGPEGRTAQNINLCVGRPTKLEPPLPNVYIT
                     EESLNVSA"
     gene            496..1290
                     /locus_tag="AC16UZ_14190"
     CDS             496..1290
                     /locus_tag="AC16UZ_14190"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6247884.1"
                     /translation="MSALEGKVAIVSGAAQGLGEAFARALSEQGAIVTAFDVQETIGD
                     VAAAIEGATGNRVLGLVADVSKRDDVERVVAATVETFGGLDLLVNNAGNWRRTPVDAS
                     WEQALADWDFIMDTNLKGLLMLSRACVPHLQSRGGGDIVNISTYYVLPAKSEGTNSPE
                     TDLYNASKWAINGFTDAWAKYLAKDNIRVNGMCMGATDTPMLRGLFDDGQLPPDLAET
                     VMRPAQIAQQMIDLLADGRSGENIGAWVGEPIVIPPHPPEHRRISG"
     gene            complement(1299..>1556)
                     /locus_tag="AC16UZ_14195"
     CDS             complement(1299..>1556)
                     /locus_tag="AC16UZ_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247885.1"
                     /translation="QVERVRAVNADVPLGIFILTGTGDNPAVKGLKAALANGYLSRFV
                     GEPEQVAEALNQLGEIGIDRVQLTELVPGSHAELAPYLLSG"
BASE COUNT          275 a          504 c          535 g          242 t
ORIGIN      
        1 agatgctcga gcagggccgt gggggcgaga tcgtcaacgt cgccaccgac cacatggtca
       61 cctgcggctc gcctttcgag atctgcccga agctcgagac ctgtccctgg gcagattcgc
      121 cccggcccac gggcgggggt gatgtgatgg acgtctacga tgcgtccaag tgggcgctga
      181 acggatttct attcgcctgg gccaaggcgc ttcgtcccca cggtatccgc gtcaacggca
      241 tgtgcatggg cgcgaccgac tcctggatga tccgggactt ctacggcttc ccgcagacgc
      301 cgggggagga gaccgaggag cagatggagg aggtgtccac ctggatggcc aagggggata
      361 cggcccaggt ggtgatgaac ctgctcaagg aaggcccaga gggccgcacg gcgcagaaca
      421 tcaacctctg cgtgggccgg ccgaccaagc tcgagccgcc gttgcccaac gtctacatca
      481 ccgaggagag tctcaatgtc agcgcttgag ggaaaagtcg ccatcgtcag cggcgccgcc
      541 caggggctcg gcgaagcctt cgcccgggcg ctgagcgagc agggggccat cgtcaccgcc
      601 ttcgacgtgc aggagaccat cggcgacgtg gcggccgcca tcgagggagc gacgggcaac
      661 cgggtactcg gattggtggc cgacgtctcg aaacgcgacg acgtggaacg ggtggtagcc
      721 gccaccgtcg agaccttcgg cggcctggac ctgctggtga acaatgccgg caactggcgc
      781 cgcacgccgg tggatgcgtc ctgggagcag gcccttgcgg actgggactt catcatggac
      841 accaacctga aggggctttt gatgctgtcc cgcgcctgcg ttccgcacct tcagtctcgc
      901 ggcggcggcg acatcgtcaa catcagcacc tactacgtgc tcccggccaa gtcggagggc
      961 acgaactcgc cggagacgga tctctacaac gcctcgaagt gggccatcaa cggcttcacg
     1021 gatgcctggg cgaagtacct ggccaaggac aacatccggg tgaacggcat gtgcatgggc
     1081 gccacggaca cgccgatgct gcgcgggctt tttgacgacg ggcagctgcc gccggatctt
     1141 gccgagacgg tgatgcggcc ggcccagatc gcccagcaga tgatcgatct gctcgccgac
     1201 ggccgctcag gtgagaacat cggtgcctgg gtgggcgagc ccatcgtcat cccgccgcac
     1261 ccgcccgagc atcggcgcat ttccggctga gccggcgctc agccggagag caggtagggc
     1321 gcgagctctg cgtgggagcc gggcaccagc tcggtgagct ggacccggtc aatgccgatc
     1381 tcgccgagct ggttcagagc ctcggccacc tgctcaggct cgcctacgaa acgggaaagg
     1441 tacccgttcg ccagcgctgc cttcagcccc ttcacggcgg gattgtcccc cgtgccggtg
     1501 aggatgaaga tgccgagcgg cacgtcggcg ttcaccgcgc ggacgcgctc gacctg
//
LOCUS       DBNHOX010000645         5438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_886663, whole genome shotgun sequence.
ACCESSION   DBNHOX010000645 DBNHOX010000000
VERSION     DBNHOX010000645.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_886663"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..686)
                     /locus_tag="AC16UZ_14200"
     CDS             complement(66..686)
                     /locus_tag="AC16UZ_14200"
                     /inference="COORDINATES: protein motif:HMM:NF015957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR6247886.1"
                     /translation="MEKLEAALHQLVDTARDQLSDRATAFIEETTATLEREFGRGTGP
                     DRPAAGRRRHGHGHRARDRATEDGIPSRSRRLYRDPGRAKIGGVCAGIARYYGIETWV
                     VRCAAVTGLIFVPQFVFPAYWIAYFLMGLPPREPASGSNGRARDDHSSPAPELGSKLS
                     PRRSLRNIQADLTQIELKLRRMETHVVSGQYELQKELNKIDEVGRG"
     gene            complement(796..1041)
                     /gene="pspB"
                     /locus_tag="AC16UZ_14205"
     CDS             complement(796..1041)
                     /gene="pspB"
                     /locus_tag="AC16UZ_14205"
                     /inference="COORDINATES: protein motif:HMM:NF006993.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HIR6247887.1"
                     /translation="MDGLAIAFFVPTVVFLTIVAPIWIFMHYRSKQRAQGALSEDERM
                     ELEALTSQAERMAERIETLESILDSETPGWRSRAQTE"
     gene            complement(1050..1790)
                     /gene="pspA"
                     /locus_tag="AC16UZ_14210"
     CDS             complement(1050..1790)
                     /gene="pspA"
                     /locus_tag="AC16UZ_14210"
                     /inference="COORDINATES: protein motif:HMM:NF015945.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HIR6247888.1"
                     /translation="MSIFSRMSDIINSNINALLDKAEDPEKMVRLIIQEMEEALVEVR
                     STSARAIADKKELARRQEYLDADALEWERKAEVAVAKGRDDLAKGALVERNKAQEAAA
                     AITKDLQILDDTLAKLNDDVTALQAKIKDAKARQNAIIMRGKAAQTRLGVRRQLNDHN
                     IDDAIQRFESYERKMDDLEGQIEAYDMGRKTLADEIAELEVDETLDEELAQLKARMGN
                     ETRSSADAAGAKDAPGREQNCPEHSANG"
     gene            1997..2995
                     /gene="pspF"
                     /locus_tag="AC16UZ_14215"
     CDS             1997..2995
                     /gene="pspF"
                     /locus_tag="AC16UZ_14215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HIR6247889.1"
                     /translation="MAAEQDRLLGESPAFLQILEEVSQAAPLEKPVLIVGERGTGKEL
                     IAARLHFLSNRWEGQFLKMNCAAISDSLLESELFGHEAGAFTGATRTHHGRFERAHGG
                     TLFLDELATTSALVQEKLLRVIEYGEFERVGGTKTLHCDVRLVAATNEDLPTRAEEGK
                     FRYDLLDRLAFDVLTLPPLREREEDILLLARHFAITMATELGREYFAGFGSAARDALM
                     THPWPGNVRELKNVVERCVYRSHPDEEVEEVVFDPFDSPYRPASQPSPPQGSTPAGNY
                     DLPLDLKQTVRELEIDLINRALENSKHNQRKAADLLKLTYHQLRGYLKKYELLKSD"
     gene            complement(3014..4408)
                     /gene="phrB"
                     /locus_tag="AC16UZ_14220"
     CDS             complement(3014..4408)
                     /gene="phrB"
                     /locus_tag="AC16UZ_14220"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HIR6247890.1"
                     /translation="MRSIVWFRNDLRVRDNPALVEACARGEVTAVTCLCPAQLRAHDV
                     GPMRIAFLLRSLGVLSHDLASIGVSLKIIERPRFADLPEALVDFARQEGATHLEFNDE
                     YPLDERRRDAEVADACAAAGIVVGRHVADCTLSPGTVLTNSGSPYTVFSPFRRRWLER
                     VDRAQLTPMDAPRRRPGKGAGDPVPVTLDGVGAGLGEDLWPAGEAAAQKALKRFLSGP
                     VLDYGDTRDSPALDATSRLSPYLAIGAISVNQCLHAAVEANGGRLDGRSEGISKWITE
                     LIWREFYRHIVALFPHVSQGKSFRRELDALEWRDAPEDYEAWKAGETGYPLVDAAMRQ
                     LNETGWMHNRLRMVTAMFLTKHLLLDWRLGERYFMEKLVDGDFASNNGGWQWSASTGT
                     DAAPYFRILNPTTQGQRFDADGAFTRRMLPVLADVPDKWLFEPKRFDADLGYPDPIVE
                     HRAARARALALFKN"
     gene            complement(4408..4824)
                     /locus_tag="AC16UZ_14225"
     CDS             complement(4408..4824)
                     /locus_tag="AC16UZ_14225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6247891.1"
                     /translation="MPLRIGFQAPGGPLIAPLWFRYRDGSFWSCSPLSSALVKSLESH
                     PEVAFDVSTNEIPYKGVRGRALAECHPASGPDVLEDLLLRYLDGTDNPLAEWLLSRVD
                     GEALIRFHIEWLTSWDFSRRMSGLERIADRDATAEL"
     gene            5089..5382
                     /locus_tag="AC16UZ_14230"
     CDS             5089..5382
                     /locus_tag="AC16UZ_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247892.1"
                     /translation="MEIVNHAREQLGPLTRQLLDAIDTESHPMEASFFAGLNVRLQHM
                     NDEGDLAELFFELSSTAFRGFAFSDHQAALIDRLLRAAEEIAFALSAPDDNAH"
BASE COUNT          996 a         1813 c         1631 g          998 t
ORIGIN      
        1 cagcaccacc atcatgaaga cactatccat agcaggcccc cggatcaggg ttggcggtgg
       61 ccgggtcagc cacggccgac ctcgtcgatc ttgttgagtt ctttctgcag ctcgtactgg
      121 ccggaaacca catgggtttc catgcggcgc agcttgagct cgatctgcgt tagatctgcc
      181 tggatgttgc gcaggctgcg acgcggcgac agcttcgaac caagctccgg ggccggcgag
      241 gaatgatcgt cgcgggcacg gccgttactg cccgacgcag gctcacgcgg cggcagaccc
      301 atcaggaaat aggcgatcca gtaggcgggg aacacgaatt gggggacgaa gatgagcccc
      361 gtcaccgccg cgcagcgcac cacccaggtc tcgatgccgt agtaccgcgc gatgccggcg
      421 cacacgccgc cgatcttggc gcgccccgga tcccggtaca gccggcggga tcgcgaggga
      481 atgccgtcct ccgttgcgcg atcacgcgcc cgatgcccgt gcccgtggcg gcgccttccg
      541 gccgccggcc ggtcgggccc tgtgccgcgc ccgaactctc gctccagcgt agccgtggtt
      601 tcttctatga aggcagtggc tcggtctgac agctgatccc tggccgtgtc gaccagctgg
      661 tgcaacgccg cttccagctt ttccacggcc tgctgaaaat cagaggctcc gtcgcgctgc
      721 cggcctgcgc caggcgatcg agaggatcca ccacctgaat tcgaatcgtt catgccttcg
      781 ctccgtccac tcagcctatt ccgtttgcgc ccgcgaccgc cacccaggcg tctcggaatc
      841 gaggatcgat tcgagggttt cgatacgctc cgccattcgc tccgcctggc tcgtcaaagc
      901 ctccaactcc atgcgctcgt cctcggacaa ggccccctgg gccctctgct tgctccggta
      961 atgcatgaag atccagatcg gcgcgacgat ggtgagaaag acgaccgtgg ggacgaagaa
     1021 cgcgatagct aagccgtcca tggttgctcc taaccgttag cagagtgttc cgggcaattc
     1081 tgttcccgcc cgggcgcgtc cttcgctccc gcggcgtcag cggatgatcg ggtttcattt
     1141 cccatgcgcg ccttgagctg ggcgagctct tcatcgaggg tctcgtccac ctcgagctcg
     1201 gcgatctcat cggcaagggt ttttcgaccc atgtcgtagg cctcgatctg accctcgaga
     1261 tcgtccatct tacgctcgta gctctcgaat cgctgaatcg catcgtcgat gttgtggtcg
     1321 ttcagctgac ggcgcacgcc caggcgcgtc tgcgccgcct tgccgcgcat gatgatcgcg
     1381 ttctgccgag ctttggcgtc cttgatcttc gcctgcagcg cggtgacgtc gtcgttcagc
     1441 tttgccagcg tgtcgtcgag gatctgcaga tcctttgtga tggccgccgc cgcttcctgt
     1501 gccttgttgc gctctacgag cgcgcccttg gcgaggtcat cgcggccctt cgccaccgct
     1561 acctcggcct tgcgctccca ctcgagggca tccgcatcga ggtactcctg acggcgagcc
     1621 agctccttct tgtctgcgat tgcccgggcc gacgtgctgc gcacctcgac gagcgcttcc
     1681 tccatttcct ggatgatgag ccgcaccatc ttctcgggat cttccgcctt gtcgagcagg
     1741 gcgttgatgt tcgaattgat gatgtcggac atgcgagaaa aaatgctcat ctcaaatctg
     1801 cctctgtagt ttcctgtgcc ggccccgtcg acgtgctgaa tctcgtgaag ccgttgcaca
     1861 tacctcttct caagttccgt gccaaaacct ttcttatacg taactatatg ttttaattga
     1921 tattatttct gacgcgaatg tcccgggcac tagaaggacg tagcagaatc ggctaaatat
     1981 tggcaagatt cgcagcatgg cagcagagca agatcgcctc ctcggggaat ctccggcctt
     2041 tctgcagatc ctcgaggagg tttctcaggc agcgccgctg gaaaaacccg tcctgatcgt
     2101 gggagaacgg ggcacgggca aggaactcat cgccgcgcgg ctgcactttc tgtccaaccg
     2161 ttgggaggga cagtttctca agatgaactg tgcggccatc agcgactcgc tgctggaaag
     2221 cgaacttttc ggtcacgagg ccggcgcgtt caccggagca acccgcaccc atcacggccg
     2281 gttcgagcga gcccacggcg gcaccctgtt tctcgacgag cttgccacca cctccgccct
     2341 cgttcaggaa aagctgctgc gcgtcatcga gtacggagag ttcgaacggg taggcggcac
     2401 caagacgctg cattgcgacg tccggctggt cgcggccacc aacgaggatc tgcccacgcg
     2461 cgcagaggaa ggcaagttcc gctacgacct cctcgatcgg ctggcgttcg acgtcctcac
     2521 gcttcccccc ctgcgggaac gcgaggaaga catcctgctc cttgccaggc acttcgcaat
     2581 cacgatggca accgagctgg gccgcgagta cttcgcgggc tttggcagcg cagcgcggga
     2641 tgccctgatg acccatccat ggcccggcaa cgtccgggag cttaagaacg tcgtggaacg
     2701 atgcgtatac cgcagccacc ccgacgagga ggtcgaagag gtcgttttcg atcccttcga
     2761 ctcaccctac cggcccgcgt cacagccctc cccgccgcag ggctcaacgc ccgcaggaaa
     2821 ctacgacctg ccactggacc taaaacagac cgtgcgggag ctggagatcg acctgatcaa
     2881 ccgcgcgctg gagaacagca agcacaacca gcgcaaggcg gccgaccttc tcaagctcac
     2941 gtatcaccag cttcgcggct atctcaagaa gtacgagctc ctcaagtccg actgactgat
     3001 cgcgcggcgc gggtcagttt ttgaaaagcg cgagagcccg cgcgcgtgcc gcgcgatgct
     3061 cgacgatggg atccgggtag ccgaggtcgg cgtcaaagcg ttttggctcg aaaagccact
     3121 tgtccggcac gtcggccagc accggcagca tccgccgggt gaaggcgcca tcagcatcga
     3181 accgctggcc ctgggtcgta gggttgagaa tccggaaata cggagcggca tccgtgcccg
     3241 tggacgcact ccactgccac ccgccgttgt tggaggcgaa gtcgccgtcg accagctttt
     3301 ccatgaagta ccgttcaccg agtcgccagt ccagcagcag gtgcttggtg agaaacatcg
     3361 cggtcaccat ccgcaaccgg ttgtgcatcc agccggtttc gttgagctgg cgcatggcag
     3421 cgtctacgag cggatagccg gtctcgccgg ctttccaggc ctcataatcc tccggggcat
     3481 cccgccattc gagagcgtcg agctcgcgcc gaaacgactt cccttgggag acatggggaa
     3541 acagcgcgac gatgtggcga tagaactctc gccagatgag ctcggtgatc cacttggata
     3601 tcccctcgga cctaccgtcg agacgccccc cgttggcttc gacagcggcg tgcagacact
     3661 ggttgaccga tatcgccccg atggcgagat agggggacag gcggctcgtg gcatcgagcg
     3721 ccggggaatc acgggtatct ccgtagtcga gcaccggccc ggagagaaag cgcttcagcg
     3781 ccttctgagc cgccgcttcc ccggccggcc acaggtcttc acccaacccg gcgccaacgc
     3841 cgtccagggt taccggaacg ggatcgccgg cgcctttgcc cgggcgccgg cggggcgcat
     3901 ccatcggcgt cagctgggcc cggtccacac gttccagcca gcggcgccgg aaggggctga
     3961 acaccgtgta gggggaaccg ctgtttgtca gcaccgttcc tggagacagc gtgcaatcgg
     4021 ccacgtggcg accaacgacg atgccggcgg ccgcgcacgc gtcggcgacc tccgcgtccc
     4081 gtctgcgctc gtccagcgga tactcgtcat tgaactccag gtgggtggcg ccctcttgcc
     4141 gcgcgaagtc tacaagcgcc tccggcaggt cggcaaagcg gggccgttcg atgattttga
     4201 gcgaaacgcc gatagacgcc aggtcatgcg agagcacgcc gagggaccgc agcaggaagg
     4261 caatgcgcat cgggcccaca tcgtgcgccc gcagctgggc gggacagaga caggtaacgg
     4321 ccgtgacctc cccccgcgcg cacgcctcca ccagcgccgg gttgtccctg acgcgaagat
     4381 cattgcgaaa ccagacgatc gagcgcatca aagttcggcc gtggcgtccc ggtcggcgat
     4441 ccgttccagc ccgctcattc gtctgctgaa gtcccagctc gtcagccatt caatgtgaaa
     4501 ccggatcagc gcttccccgt cgactctcga cagcagccat tccgccagcg ggttgtccgt
     4561 gccgtcgaga tatctcagca gaagatcctc gaggacgtcg gggccgctag ccgggtgaca
     4621 ctccgccaac gcccgaccgc ggactccttt ataggggatc tcgttcgtgg atacgtcgaa
     4681 ggccacctcc ggatggctct cgagcgactt gaccagggcc gacgacaggg ggctgcagga
     4741 ccagaacgaa ccatcccgat atcggaacca caacggcgcg atcagcggcc cgccgggcgc
     4801 ttggaagcca atgcgcagag gcacgcgagt agattcgaaa aatcgctcca cctcgcgccc
     4861 tcgccaggcc ccatagtgtt tctcaggctg aaaattcaat gacccaccct ccgtagcaag
     4921 aacccgatgt tatctagatt ccgataaaaa agccgtgaaa gtgcgcggcc ggcaaggcga
     4981 ggcgcggccg gcaaggcgcg gccggcggca tccggcaagc gcagagcggc cacggccctc
     5041 accgaggttt tgacgtcaat ttcaccccgc caccgccagc atcgcaccat ggaaatcgtg
     5101 aaccatgccc gtgagcaact cgggccgctg acgcggcaac tgctcgacgc catcgacacc
     5161 gagtcgcacc ccatggaagc ctcgtttttc gctggcctga acgtccggct tcagcacatg
     5221 aacgacgagg gtgatctggc tgagctcttt ttcgagcttt ccagcacggc gtttcgcggt
     5281 ttcgccttct ccgaccacca ggccgccctg atagaccggc tgctgcgcgc cgccgaagag
     5341 atcgccttcg ccctcagcgc accggacgac aacgctcact agagcgccgg gccgcaattt
     5401 cgcctgctac cgttcagtgg catgatgccc acatcgtg
//
LOCUS       DBNHOX010000646         2717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1016565, whole genome shotgun sequence.
ACCESSION   DBNHOX010000646 DBNHOX010000000
VERSION     DBNHOX010000646.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1016565"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="AC16UZ_14235"
     CDS             <1..357
                     /locus_tag="AC16UZ_14235"
                     /inference="COORDINATES: protein motif:HMM:NF017868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HIR6247893.1"
                     /translation="VRLEFHKTSRVHAGVGHASLAFAPGGVVEGVLYELAGADEIFAM
                     DRFESAPVNYSREIVQIQTEEGALAGWTYFANPAVIVPGLKPPRSYLEHLLAGRPFLS
                     DPYFRMLESWECADDR"
     gene            339..827
                     /locus_tag="AC16UZ_14240"
     CDS             339..827
                     /locus_tag="AC16UZ_14240"
                     /inference="COORDINATES: protein motif:HMM:NF016222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HIR6247894.1"
                     /translation="MRGRPVISDRAIAGRINPHIRSRYATLIVGGAREPLYQPAIGRS
                     LAIIRYTRDYAQSVLHEVAHWCLADGERRLLEDYGYWYLPPPRTPEQQAAFFAAEERV
                     QALESLLAAACGLRFHVSADQPGTEVGAFAASVRRRAARWRETGLTGRAAEIHGLLGR
                     PW"
     gene            848..1924
                     /locus_tag="AC16UZ_14245"
     CDS             848..1924
                     /locus_tag="AC16UZ_14245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6247895.1"
                     /translation="MESVDCVVIGAGVVGLACARRLALDGHEVVVVERRGRIGEETSS
                     RNSEVIHAGIYYPTDSLKAKLCVAGKARLYEYCRERALPHRRCGKIIVASEPAQEAAL
                     QGYAIQAERNGVRLEWLDAAQVNALEPAVRASAGLLSESTGIIDSHAYMVSLQGDLES
                     AGGVVAFNTEVLDLGIERGLRVLTSTVTLECRRLVNAGGLWAPDLARRLDPSAPRAFY
                     ASGHYFTYSGPAPFERLVYPVAEPGGLGVHVTLDLAGQVKFGPDVTWLDGVDYTFDET
                     RLAGFVESIRRYFPAVEPARLQPGYTGIRPKIAGPGEPNADFRIDGPETHGIGGLVNL
                     LGIESPGLTASLAIADLVAERLSA"
     gene            1921..>2717
                     /locus_tag="AC16UZ_14250"
     CDS             1921..>2717
                     /locus_tag="AC16UZ_14250"
                     /inference="COORDINATES: protein motif:HMM:NF013663.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-NAD kinase family protein"
                     /protein_id="HIR6247896.1"
                     /translation="MICVGLLVNPVAGIGGAVALKGSDGADVQRVAAARGGEPRGEAR
                     ARRALRAAGPATAQLRWLTWDGGMGADLLRSEAVICEVLGAPGDPPGPSDTRQAATAF
                     AEAGADLLVFAGGDGTARDILDAVGERLPVLGIPAGVKMHSGVFAITPEAAGEILVRL
                     VEGGLVRSCRADVRDLDETALRDGVVRPRYFGELLVPELGRFLQRTKESGRENEAMAL
                     GEIVADVVERVRASDSSMSFVLGPGSTVAAIKDELGMKATLLGVDVYR"
BASE COUNT          401 a          861 c          965 g          490 t
ORIGIN      
        1 gtgaggctcg agtttcacaa aacttcgcgc gttcacgccg gcgtgggtca tgccagcctc
       61 gcctttgcgc ccggcggcgt ggtggagggc gtgctctacg agctcgccgg cgccgatgaa
      121 attttcgcga tggaccgctt cgagtctgcg ccggtgaact acagcaggga gatcgtgcag
      181 attcagaccg aggagggcgc gctggccggc tggacctact tcgcgaaccc cgcggtgatc
      241 gttcccggcc tgaagccgcc gaggagctac ctcgagcacc ttcttgccgg tcggcccttc
      301 ctgtcggacc cgtactttcg catgctcgag agctgggaat gcgcggacga ccggtgatca
      361 gcgatcgcgc cattgccggc cgcatcaacc cccacatccg ctcgcgctat gcaacgctga
      421 tcgtgggcgg ggcaagggag ccgctgtatc aaccggcgat cggcaggtcc ctcgccatca
      481 ttcgctacac ccgcgattat gcccagagcg tcctgcacga ggtcgctcac tggtgcctgg
      541 ccgatggcga gcgccggctt ttagaggact acgggtactg gtatctgccg ccgccccgaa
      601 ccccggaaca gcaggcggcg ttcttcgcgg ctgaagagcg cgttcaggcg ctggaatctc
      661 tccttgccgc tgcttgtggt ctgcgctttc acgtcagcgc cgatcagcct ggcactgagg
      721 tgggggcttt cgccgcatcg gtccgccgca gggccgcgcg ctggcgtgag acgggcctca
      781 cgggacgggc ggcggaaatc cacgggcttc tcgggcgacc ctggtgacga cgagcgggca
      841 gggcgcggtg gagagcgttg attgcgtcgt tatcggggcg ggcgtggtgg gcctggcctg
      901 tgcccggcgt cttgcccttg acggtcacga ggtggtggtt gtcgagcgtc gcgggcggat
      961 cggcgaagaa accagctcga gaaattccga ggtgattcac gccggcatct actaccccac
     1021 cgattccttg aaggcgaagc tctgcgtagc cggtaaagca cggctctacg agtattgccg
     1081 ggagcgggca ctgcctcacc gtcgctgcgg caagatcatc gtcgcctccg agcctgccca
     1141 ggaagcggcg ctgcagggat acgcgatcca ggcggaaagg aacggtgtgc ggctcgagtg
     1201 gctggacgct gctcaggtga atgccctcga accggcggtg cgggccagcg cggggctgct
     1261 ttccgagtcc acgggcatca tcgactccca tgcctacatg gtgagtctgc agggggacct
     1321 ggaatcggca ggcggcgttg ttgccttcaa tacggaagtt ctcgatctcg ggatagagcg
     1381 gggcctgagg gtgctgacga gcaccgtgac gctcgagtgc cgccgcctgg tgaatgcagg
     1441 tgggctctgg gcaccggacc tggcccgtcg tctggatcct tccgcgcccc gggccttcta
     1501 cgcaagcggc cattatttta cctacagcgg cccagcaccc ttcgagcgtc tcgtctaccc
     1561 ggttgcagag ccgggcgggc tgggcgtgca cgtcacgctg gatctggccg gccaggtgaa
     1621 gttcgggccc gacgtgacgt ggctcgatgg ggtggactac accttcgacg agacgcgcct
     1681 ggcaggcttc gttgaatcca tccgccggta tttcccggcg gttgagcccg cccgcctcca
     1741 gcccggctac accggaatac gcccgaagat cgccgggccc ggagagccca atgccgattt
     1801 ccgcatcgac ggtcccgaga cccacggcat tggcggcctg gtgaaccttc tcggcatcga
     1861 gtctccgggt ctgacggcct ccctggccat cgccgatctc gtcgcggaga ggctgtcggc
     1921 atgatttgcg tggggctgct tgtcaatccc gtcgccggca ttggcggtgc cgtggctctc
     1981 aagggcagcg acggtgcgga cgttcagcgg gtggcggctg cccggggcgg cgagcctcgt
     2041 ggagaagcgc gcgcacggcg tgctcttcgc gcggccgggc cggcgaccgc gcagctgcgc
     2101 tggctgacct gggacggggg catgggcgca gatctgctac ggtcggaggc ggtgatctgc
     2161 gaggtgctcg gcgcgccggg cgatccgcca gggccgtcgg atactcgtca ggccgcaacc
     2221 gccttcgccg aagccggtgc ggacctgctg gtcttcgccg gcggagacgg gaccgcgaga
     2281 gacatcctcg atgccgtggg cgaacgcctt cccgttctcg gtatcccggc aggggtcaag
     2341 atgcactcgg gcgtctttgc gatcacgccg gaggcggctg gcgagatact cgttcgtctc
     2401 gtggagggcg gcctggttcg gtcctgccgc gcggacgttc gcgatctcga cgagacggcg
     2461 ctgcgagacg gcgtggtgcg cccgagatac ttcggtgagc ttctcgtgcc cgagctcggc
     2521 cgttttctgc aacgcaccaa ggagagcggc cgcgagaacg aggcgatggc gctgggcgag
     2581 atcgtcgcgg acgtggtgga gcgcgttcgc gcgtcggatt catccatgtc cttcgttctg
     2641 ggtcccggca gcaccgtagc ggccatcaag gatgagctgg gaatgaaagc caccctgctc
     2701 ggcgttgacg tctatcg
//
LOCUS       DBNHOX010000647         2586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_503239, whole genome shotgun sequence.
ACCESSION   DBNHOX010000647 DBNHOX010000000
VERSION     DBNHOX010000647.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_503239"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="AC16UZ_14255"
     CDS             complement(<1..397)
                     /locus_tag="AC16UZ_14255"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR6247897.1"
                     /translation="MSFDVALKLLNERSWKPVPLAEPIPVSSLPTPALLIDESALDRN
                     IKRMADFVAARGKNARPHGKTHKCPIIAARQIEAGAVGICAAKVSEAAVFVNAGVEPV
                     LITSPVTTAAKAELIVALARKGRVSVVVDG"
     gene            complement(406..1242)
                     /gene="galU"
                     /locus_tag="AC16UZ_14260"
     CDS             complement(406..1242)
                     /gene="galU"
                     /locus_tag="AC16UZ_14260"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIR6247898.1"
                     /translation="MIRKCLFPVAGYGTRFLPATKAMAKEILPVLNKPLVQYAVEEAL
                     EAGLTDVGFVTGRGKRAIEDHFDTSYELEHQIAGTGKEKALDGIRRIMEECTFSYTRQ
                     VNMRGLGDAILKGRTLVGENPFGVVLADDLCFGNGHREGGVLKQMVKLFKQFRCSIVA
                     IEEVPEDRVSSYGVIAGEALSDRLVRITDMVEKPSPENAPSNFGIIGRYILTPDIFEI
                     LENTEPGKNGEVQITDALLQQAKDGCVIAYRFQGKRFDCGSVAGFVDATNYCYEHLYT
                     ET"
     gene            complement(1377..1649)
                     /locus_tag="AC16UZ_14265"
     CDS             complement(1377..1649)
                     /locus_tag="AC16UZ_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247899.1"
                     /translation="MNDRFESLPLFPDEAPPAKRRQVDAGRSEPGRGRASAAARSLGS
                     RDPHLIAANRLARAIRRLPDHSPEQTRTGQAICRHLLALLEEAERR"
     gene            complement(1716..>2586)
                     /locus_tag="AC16UZ_14270"
     CDS             complement(1716..>2586)
                     /locus_tag="AC16UZ_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6247900.1"
                     /translation="ERVVGQDEAVRIVADAVRRSRAGLSDPNRPNGSFMFLGPTGVGK
                     TELCKALAQVLFDSDEALVRVDMSEFMEKHSVARLIGAPPGYVGYEEGGFLTERIRRR
                     PYSLILLDEIEKAHPDVFNILLQVLDDGRLTDGHGRTVDFRNTVLVMTSNLGSEAIQS
                     LTEAGNADRIKDTVLGVVSQHFRPEFLNRVDDIVVFHPLGREHVAEIAKLQFDILRRR
                     LEEQDLTIDIAPAAVEALVEEGYDPVYGARPLKRVIQRRIENPLASRILGGDFLPGST
                     IRVGYADGDYSFD"
BASE COUNT          487 a          822 c          799 g          478 t
ORIGIN      
        1 ggccgtctac caccacgctc acacggcctt tccgggcaag cgcaacgatg agctcggcct
       61 tcgctgcggt agtgacagga gaggtgatca gaacaggttc cacgccggca ttgacgaaga
      121 cggcggcttc ggaaaccttc gccgcacaga tccccacggc accggcttcg atctgccgcg
      181 ccgcgatgat cggacacttg tgggttttgc cgtgtgggcg ggcgtttttg cccctggcgg
      241 cgacgaagtc cgccatgcgc ttgatgttgc gatccagcgc gctctcgtcg atcagcaacg
      301 cgggcgtcgg cagggagctg accgggatcg gctcggcaag cgggaccggc ttccaggatc
      361 gctcgttgag cagctttaaa gcaacgtcaa acgacatgcg cctggctagg tttccgtata
      421 gagatgctcg tagcagtagt tggtcgcgtc gacgaacccg gccaccgaac cgcagtcgaa
      481 gcgcttcccc tggaaccggt aggcgatgac gcagccgtcc ttagcctgct ggagaagcgc
      541 gtccgtgatc tggacctcac cgttcttgcc gggctccgtg ttctccagaa tctcgaaaat
      601 gtcaggggtg agtatgtagc gtccgatgat gccgaagttg ctgggcgcat tctcaggact
      661 cggcttctcc accatgtccg tgatgcgtac caggcggtcg ctaagcgctt ccccggcgat
      721 gacgccatag ctgctcaccc ggtcctcggg cacctcctcg atcgccacga tgctgcagcg
      781 aaactgcttg aagagtttca ccatctgctt gaggaccccg ccctcgcggt ggccgttgcc
      841 gaaacacaga tcgtcggcca gcaccacgcc gaaggggttc tcgccgacca gcgtgcgtcc
      901 tttcagaatc gcatccccca ggccccgcat gttgacctgc cgcgtgtacg agaacgtaca
      961 ctcctccatg atccggcgga taccgtccag ggccttttcc ttgccggtcc cggcgatctg
     1021 atgttccagc tcatagctcg tgtcgaagtg gtcctcgata gcgcgcttgc cccgccccgt
     1081 cacgaagccg acgtcggtga gaccggcctc cagggcttcc tccactgcgt actgaacgag
     1141 aggcttgttg aggacgggaa ggatttcctt ggccatcgcc ttggtcgccg gcagaaaacg
     1201 ggtcccgtaa ccggcgacgg gaaacaaaca cttcctgatc atgaaatcca tctcaaagaa
     1261 tcagtacaaa tgacgatggt aggacggtta gcgtccacta ccatggacga accgcacaga
     1321 ttgaacaaca aacgacggcg gagcgacaac ctgacccgac ggtgaatgcg ggaggatcag
     1381 cgacgctcgg cttcctccag gagggccaga aggtgccgac agatcgcctg gccggtccgc
     1441 gtctgctccg gtgagtggtc ggggagccgg cgaatggctc gggcgagccg gttggccgca
     1501 atcagatgag gatcccgcga acccaggctt cgggcggctg cgctcgcgcg gcctcgcccc
     1561 ggttcagacc ggcctgcgtc cacctggcga cgcttcgccg gcggcgcctc gtccgggaac
     1621 agcggcaggg attcaaagcg gtcattcatg acgtggaaat gtaccgaata ctgtataaac
     1681 atacaatact cggcacatcg tttttcgaca ggttattagt cgaatgagta gtcaccgtcc
     1741 gcataaccga cccggatggt ggaccccggg aggaagtcgc cccccagaat ccggctggcc
     1801 agggggttct cgatgcgtct ttgaatcacg cgcttgagtg gccgggcacc gtaaaccggg
     1861 tcgtagccct cctccaccag cgcttccacc gcggccggag cgatgtcgat cgtgaggtcc
     1921 tgttcttcca accgccggcg cagaatgtcg aactggagct tggcaatctc ggcaacgtgc
     1981 tcgcgcccga gcgggtgaaa cacgacgatg tcgtccaccc gattcaggaa ctcggggcgg
     2041 aagtgctgcg agaccacgcc caggaccgta tccttgattc ggtcggcatt gcccgcctcc
     2101 gtgagcgact gaatggcttc ggagccgagg ttcgacgtca tgaccagaac cgtgttccga
     2161 aagtcgaccg ttctcccgtg cccgtccgtg agacgcccat cgtctaacac ctgcagcaga
     2221 atgttgaaca cgtcggggtg cgccttttcg atctcatcga gcaggatcag cgagtagggc
     2281 cggcggcgta tccgctcggt gagaaacccg ccctcctcgt agcctacgta tcccggaggg
     2341 gcgccgatga gccgcgcaac ggagtgtttc tccatgaact cggacatgtc cacgcgcacg
     2401 agcgcttcgt cgctgtcgaa gagcacctgt gccaacgctt tgcacagctc ggtcttgccc
     2461 acgccggtgg ggccgaggaa catgaacgag ccgttggggc ggttgggatc cgacagcccg
     2521 gcacgggagc ggcggacggc atcggcaacg atgcgcaccg cctcgtcctg gcccacgacg
     2581 cgttcg
//
LOCUS       DBNHOX010000648         2382 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1016784, whole genome shotgun sequence.
ACCESSION   DBNHOX010000648 DBNHOX010000000
VERSION     DBNHOX010000648.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1016784"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(411..1169)
                     /locus_tag="AC16UZ_14275"
     CDS             complement(411..1169)
                     /locus_tag="AC16UZ_14275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR6247901.1"
                     /translation="MTENEDVDAFRELIEAAKNVVFFTGAGISTESGIPDFRSPGTGL
                     WNKIRPIEFQDFVASEDKRREYWQRHFTGERPMANAKPNKGHDGVQRLIASGKATAVI
                     TQNVDNLHQDSGIPEDQVIELHGNASYATCLACGTRYELADLEQQFHRHGNVEPCGRC
                     GGIVKSATISFGQAMPDAAMLRAQAATEACDLMIVIGSSLVVYPAAGFPEYAKRRGAG
                     LVILNREETPLDPFADLVLHRDIGPTMSYVTGIN"
     gene            complement(1236..1880)
                     /locus_tag="AC16UZ_14280"
     CDS             complement(1236..1880)
                     /locus_tag="AC16UZ_14280"
                     /inference="COORDINATES: protein motif:HMM:NF019544.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol phosphotransferase CPT family
                     protein"
                     /protein_id="HIR6247902.1"
                     /translation="MQIPSPRMQTGKIIFLNGSSSAGKTTIAVTLQQLLEEPFQHIAL
                     DQFRDGMPGRFRGLNSPEGTPGARGLNVVPVERDGEKMTEIRFGEVGEQILRGMRRAI
                     SAFAAAGNNVIVDDLLFKPDYLHDYAKALADHDAWLIGVRCSLDVVNEREAQRPGRFP
                     GTATSHFHQVHEHGAQYDLEVDTTTTTPRACAELIIERLARPPEALERLRSARP"
     gene            1883..>2382
                     /locus_tag="AC16UZ_14285"
     CDS             1883..>2382
                     /locus_tag="AC16UZ_14285"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF036823.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="HIR6247903.1"
                     /translation="MLHHNSGMQGEPGQALAAELDIRIPRMDGLSLRVAPVADFEHEL
                     HGPELEAVARAVPKRRQEFATGRHLARAAMRELGLPAAPVPRRADRSPVWPDGCLGSI
                     THTNALAGVAVARAGVVEGIGIDLEESHRVSAELHDKLFTPTELGRYVDAPPGWADLL
                     FSAKESV"
BASE COUNT          369 a          773 c          799 g          441 t
ORIGIN      
        1 cagatgccgc gacggatctc gccaatgtct ggatcttcgc ccggcaagcg caaggcccct
       61 gaactcagcc atcggatggt agcccggctt gctagaatcg gtcacccatg gaggtcaccc
      121 taaaccgctt cgttcacgcc cttcgcagcg ccgaggtcga ggtttcgccc gcggagaccc
      181 tggacgggtt cgccgtggtg cgtcaggtgg gtatagccga tccggttctg cttcaggacg
      241 ccctggggct agcccttgcc aagacccgcg aagagaaagc gcgctttgcc gactgcttcg
      301 agcgcttggt tcgccagctc gccttcgcct ccggatccgg cggcgaagca gccgtgcgcg
      361 gggctcaagg acatcgagcg cttcgccgac cgtctgctca gcgtgaccgg ctagttgatg
      421 ccggtgacgt aggacatcgt ggggccgata tcccggtgga gaaccagatc tgcgaacggg
      481 tccagcggcg tttcttcccg gttcaggatg acgagacccg cgccccgtcg tttggcgtat
      541 tccgggaaac cggctgccgg gtagacgacc agggaagagc cgatcacgat catcaggtcg
      601 caggcttccg ttgccgcctg ggcccggagc atggccgcat cgggcatggc ctggccgaag
      661 gaaatggtcg cggacttgac gatgccgccg cagcgaccgc agggctcgac gttgccgtgg
      721 cgatgaaact gctgctccag atcggcaagc tcgtaacggg ttccgcaggc gaggcaggtg
      781 gcgtagctgg cgttgccgtg cagctcgatg acctgatcct ccggaatgcc cgagtcctga
      841 tgcagattgt ccacgttctg ggtgatcacg gcggtcgcct tgcccgaggc gatcagccgc
      901 tgaaccccgt cgtggccctt gttcggtttg gcgttggcca tgggcctctc gccggtgaag
      961 tggcgttgcc agtactcccg gcgtttgtct tcgctggcaa cgaagtcctg aaactcgatg
     1021 ggtctgatct tgttccacag gccggtgccc gggctgcgaa aatcgggaat tcctgactcg
     1081 gtgctgatgc cggctcccgt gaagaacacc acgttcttcg cggcttcgat cagctcgcga
     1141 aacgcgtcga catcctcgtt ctcggtcatt ggcctgcctc cctgtggggt tgtcgcgtgt
     1201 tcagccccgc gttgtcgatt gcctatgtcc caggatcagg gtctggccga gcgcagtcgc
     1261 tcgagcgctt ccggcggccg ggccaggcgt tcgatgatga gctccgcgca cgcgcgcggg
     1321 gtcgtcgtcg tggtgtcgac ctccaggtcg tactgcgcgc cgtgctcgtg cacctggtga
     1381 aagtgcgagg tggcggtgcc gggaaagcgc ccggggcgct gcgcttcgcg ttcgttgacc
     1441 acgtcgaggc tgcagcgcac gccgatcagc caggcgtcgt gatctgccag ggccttcgcg
     1501 tagtcgtgca ggtagtcggg cttgaagagc agatcgtcaa cgatcacgtt gttgccggcg
     1561 gcggcaaagg cggagatcgc ccgacgcatg cctctcaaga tctgctcacc cacctcgccg
     1621 aagcggatct cggtcatctt ctccccgtcg cgctctaccg gaacgacgtt caggccccgg
     1681 gccccgggcg ttccctcggg ggaattcagc ccgcggaaac ggcccggcat cccgtcgcgg
     1741 aactggtcca gggcgatgtg ctgaaagggc tcctcgagca gctgctggag cgtgacggcg
     1801 atggtggtct taccagcgct cgaagagccg ttcagaaaga tgatcttgcc ggtctgcata
     1861 cgcggcgatg gtatctgcac ccatgctgca ccacaattcg ggcatgcagg gagagcctgg
     1921 acaagcgttg gcggccgagc tcgacatccg cataccgcgg atggatggcc tgagcctgcg
     1981 cgtcgccccc gtggcggatt tcgagcacga gctccatggc ccggagctag aggctgttgc
     2041 ccgggccgtg ccaaagcggc ggcaggaatt cgccaccggc cgtcatctgg cccgtgcggc
     2101 catgcgcgag ctcggcctgc cggccgcccc ggtcccgcgc cgggccgacc gcagccccgt
     2161 atggcccgac gggtgccttg gcagcatcac ccacaccaat gccctggccg gcgtcgccgt
     2221 tgcccgagcc ggcgtcgtgg aaggcatcgg catcgatctc gaggaatcgc accgggtgtc
     2281 tgccgagctg cacgacaagc tcttcacccc gacagagctc ggccggtacg tggatgcgcc
     2341 gccggggtgg gccgatcttc tgttcagcgc caaagaatcc gt
//
LOCUS       DBNHOX010000649         2158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1659121, whole genome shotgun sequence.
ACCESSION   DBNHOX010000649 DBNHOX010000000
VERSION     DBNHOX010000649.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1659121"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="AC16UZ_14290"
     CDS             <1..1118
                     /locus_tag="AC16UZ_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HIR6247904.1"
                     /translation="LFRARRLERFLETPARIFYKYEGVSPAGSHKPNTAVPQVWYNAQ
                     EGITKLTTETGAGQWGSSLAFACAQFGLECEVWQVAASYHAKPYRRTMMEIWGGKVHP
                     SPSEVTEFGRSLLAENPDHPGSLGIAISEAVSQAVADPQTRYALGSVLNHVLLHQTII
                     GEEALLQLAKVGETPDVLVGCTGGGSNFGGLSFPFLREKLAGRINPTIRCVEPTACPT
                     LTKGQYRYDFGDTAGLTPLMKMHTLGHSFIPEPIHAGGLRYHGMAPLVSHLYELGMVE
                     AIAIPQTECFEGALQFARTEGIVPAPEPTHAIAAAIREALACRESGEEKVILTALCGH
                     GHLDLASYDKYLHGEMVDLDLSDEVIEAAMKDVPVLA"
     gene            1204..>2158
                     /locus_tag="AC16UZ_14295"
     CDS             1204..>2158
                     /locus_tag="AC16UZ_14295"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6247905.1"
                     /translation="MNSTRSGLGRRLSAPAAFLGLACAFSGVAQSAHSAASSVIEEIV
                     VTARMREEGLQSAPIAISAFTEETLDYRGVERLDEIAKFVPSLTLENNPSFGGASNSA
                     AIYIRGIGQKEFLPTTEPGVGLYVDGVYVARSVGAILDIIDVERLEVLRGPQGTLFGR
                     NTIGGAISITTVKPEPGGEFEAMVSGALGTDDLVHLRGSAHLPLSDTLAARLSVASFQ
                     QDGYVDRADGIDLGEDDTLTGRLAIAWEPNERFRADLSFEATRDRENGPPMELIGIDF
                     TDLSQLQGVVLAPPPPMAFIHNVTAAAAGPGQPCAVTDVNGN"
BASE COUNT          400 a          707 c          672 g          379 t
ORIGIN      
        1 cgttgttccg agcacggcgc ctggaaaggt tcctggagac gcctgccaga atattctaca
       61 aatatgaggg cgtgagcccg gcgggttccc acaagccgaa caccgccgtt cctcaggtct
      121 ggtacaacgc ccaggaaggc atcaccaagc tcaccaccga gaccggcgcc ggccagtggg
      181 gcagctcgct cgcctttgcc tgcgctcagt tcggcctgga gtgcgaggtc tggcaggtgg
      241 cggcttccta tcatgccaag ccttaccggc ggacgatgat ggaaatctgg ggcggcaagg
      301 tgcacccgag cccctctgaa gtcacggagt tcggccgttc cctgcttgcc gagaaccccg
      361 accatcccgg atcgctgggg atcgcaatct ctgaagcggt gagccaagcg gtcgccgatc
      421 cgcagacacg ttacgcgctc ggcagcgtgc tcaatcacgt gctgctccac cagacgatca
      481 tcggagagga agcactgctg cagcttgcga aagtcggcga aaccccggac gtgctggtgg
      541 gttgcaccgg cggcggatcg aatttcggcg ggctgtcttt tccgttcctg cgcgagaaac
      601 tcgccggccg catcaaccct accattcgct gcgtggaacc cactgcctgc ccgactctga
      661 ccaaaggtca gtaccgctac gactttggcg acaccgcggg cctgacgccg ctgatgaaga
      721 tgcacacgct cggccactct ttcattccgg agccgatcca cgcaggcggc ctgcgctacc
      781 atggcatggc gccgctggtc tcgcatctct acgagctcgg catggtggag gcgatcgcaa
      841 ttccacagac ggagtgtttc gagggcgcgc tgcagttcgc ccgtaccgaa ggcatcgtgc
      901 cggcccccga gccgactcat gccatcgcgg ccgccattcg cgaggcactc gcctgccggg
      961 aatccggtga ggaaaaggtc atcctgaccg ccttgtgtgg ccacggccac ctggatctgg
     1021 cgtcctatga caagtatttg cacggagaaa tggtcgatct cgatctctcc gacgaagtca
     1081 tcgaggcggc catgaaggac gtgcccgtcc tcgcatgagc gggcctagac gagcgaacac
     1141 ccgcttttaa acgagcacca ccgaacgaac gacgacagaa caagcaatca ggggagaacc
     1201 atcatgaact ctactcggag cggcctgggc cgccgtctgt ccgcgccggc cgcgtttctc
     1261 ggcctggcct gtgctttttc gggagtcgct cagtcggcgc acagcgctgc gtccagcgtg
     1321 atcgaggaaa tcgtggtcac tgcccgcatg cgtgaagagg ggctgcagag cgcgcccatc
     1381 gccatttcgg cattcaccga ggagacgctc gattaccggg gggtggaacg tctggacgag
     1441 atcgcgaaat tcgtgcccag cctgacgctg gagaacaacc cgagtttcgg tggtgcctca
     1501 aactctgccg ccatttatat ccgcggcatc ggtcagaaag agtttctgcc caccacggaa
     1561 cctggcgtgg gtctgtacgt ggacggcgtg tacgtggctc gctcggttgg cgcgatcctg
     1621 gacatcatcg acgtggaacg cctggaagtc ctgcgcggcc cccagggcac cctgtttggc
     1681 cggaacacca tcggcggcgc catatccatc accacggtga aacccgagcc aggcggcgag
     1741 tttgaagcca tggtatccgg cgctctgggc accgacgatc tggtgcacct gcgcggttca
     1801 gcccacctgc cgctgagcga cacgctggct gccaggctgt cggtagcttc cttccagcag
     1861 gacgggtacg tggaccgtgc cgacggcatc gatctgggcg aggacgacac gttgaccggg
     1921 cgtttggcaa tcgcgtggga gcccaacgaa cgcttccgcg ctgatctgag cttcgaggcc
     1981 acacgggacc gcgaaaacgg tccgcccatg gagctgatcg gcatcgactt caccgacctc
     2041 agccagctgc agggtgtggt tctggcaccg cctccgccca tggcattcat tcacaacgtc
     2101 accgccgccg cggccgggcc gggccagccc tgcgccgtca ccgacgtcaa cggcaacg
//
LOCUS       DBNHOX010000650         1828 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1209008, whole genome shotgun sequence.
ACCESSION   DBNHOX010000650 DBNHOX010000000
VERSION     DBNHOX010000650.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1209008"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1728
                     /locus_tag="AC16UZ_14300"
     CDS             <1..1728
                     /locus_tag="AC16UZ_14300"
                     /inference="COORDINATES: protein motif:HMM:NF042573.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HIR6247906.1"
                     /translation="EANVRAIPDSPMPGPFRADRTPWVRDALRIAADPEVQLITVLAS
                     IQSGKSLFARLLTCWIAEHAPGPTLLLQANDPEAKDFALRYLRPVFKNCPPVMARLKA
                     DDMERSTTIDFDRFPLYCRGASNESNLQRLSIRYVIGDECWLWPPGHLQEASARVTAF
                     GWMGKRVFMTQGGTLGGKGGEFHALHDTTDQRDWNFRCPKCDHLQPWLWEMVRFPEEA
                     KASGSWDLNAVADGTKYECAGCHTMLDDNAGTRAEANARGEFVATNPLAYHGKVGLHW
                     NSLATMSWGELGVMMLKAKEAADVYGDNEPRRIFKQKRLAMGWQEEGGEIVADAAASE
                     YNLGDAWEDEAFITGKGKVVDGKDAPAGSIPFRTMGVDVQRGHFWVVVRSWAKTGHSR
                     LYAFAKVETWGGVEDFAKKNNVHKALVMVDSGDNTTEVYRETARRGWKCARGSGNEDF
                     AVTDRDGKTTRRFYSERQRIQVPGLPGQPAVLISWSNLAGKDLLHGMRVRRLHTFARN
                     VDPFYAEMMASEVRVKDKRTGKPMWIMPQGKKDNHAWDCELLCLLAAVRWGIGSRGET
                     AQAEGVDAA"
BASE COUNT          322 a          531 c          675 g          300 t
ORIGIN      
        1 gaggcgaacg ttagggccat ccctgactcg ccgatgccgg ggccgttcag ggccgaccgt
       61 acgccgtggg tgcgggacgc gctgcgcatc gccgccgacc cagaggttca actgattacc
      121 gtcctcgcga gcatccaatc gggcaagtcg ctgttcgccc gtctgctgac ctgctggatc
      181 gccgagcacg cgcccgggcc gacgctgttg ctccaggcta acgaccccga ggcaaaggac
      241 ttcgccctgc gttaccttcg cccggtattc aagaactgcc cgccggtgat ggcacggctg
      301 aaggccgacg acatggagcg ctcgacgacc atcgacttcg accgcttccc gctgtactgc
      361 cgcggggcgt cgaacgagtc gaaccttcag cgcctgtcca tccgttacgt catcggtgac
      421 gagtgctggc tgtggccgcc cgggcatttg caggaagcga gcgctcgcgt gacggcgttc
      481 ggctggatgg gcaagcgtgt gtttatgacg cagggcggga cgcttggcgg caagggtggc
      541 gagttccacg cgctgcacga cacgaccgac caacgggatt ggaacttccg ttgcccgaag
      601 tgcgaccacc ttcagccctg gctgtgggag atggttcgct tccccgagga ggcgaaggcg
      661 agcgggtcgt gggatttgaa cgccgtggcc gacggcacga agtacgagtg cgctggctgt
      721 catacgatgt tagacgacaa cgccgggacg cgggccgagg cgaacgcccg gggcgagttc
      781 gtggcgacca atcctctggc ctatcacggc aaggtcggct tgcattggaa cagcctcgcg
      841 acgatgagct ggggcgagct gggcgtgatg atgctgaagg caaaggaggc ggcggacgtg
      901 tacggcgaca acgagcctcg gcgcatcttc aagcagaagc ggctggcgat ggggtggcag
      961 gaggagggcg gcgagatcgt ggcggacgcg gcggccagcg agtacaacct gggcgacgcg
     1021 tgggaggacg aggcgttcat caccgggaag ggcaaggttg tcgacgggaa ggacgcgccg
     1081 gctgggagca tcccgttccg cacgatgggg gtcgacgttc agcgaggcca tttctgggtc
     1141 gtcgtccgca gctgggcgaa gaccgggcat agccgccttt atgccttcgc caaggtcgag
     1201 acgtggggcg gggtcgagga ctttgccaaa aagaacaacg tccacaaggc gctggtgatg
     1261 gtcgactcgg gcgacaatac gaccgaggtc taccgcgaga cggcccgccg aggctggaag
     1321 tgtgcccgag gttcgggtaa cgaggacttc gcggtgaccg atcgggacgg caagacgacc
     1381 cggcggtttt actccgagcg ccagcgcatc caagtcccgg ggctacccgg tcagccggcg
     1441 gtgctcatct cctggtcgaa cttggcgggg aaggacttgc tccacgggat gcgggtcagg
     1501 cgcttgcata cgttcgcccg gaacgtcgac ccgttctatg ccgagatgat ggcgagcgag
     1561 gttcgggtaa aggacaagcg cacgggcaag ccgatgtgga tcatgccgca ggggaagaag
     1621 gacaaccacg cctgggactg cgagcttctc tgcctgttgg cggccgtgcg ctggggcatc
     1681 ggtagccgcg gcgagacggc ccaggccgaa ggggtggatg ccgcttgacg caaggttgtc
     1741 gggtgcttgt gtgttcatag gccaccggcc cgggacttgc gcgtggggcg tgggttggaa
     1801 caaccgggtc ggtggctccc tgttgcca
//
LOCUS       DBNHOX010000651         3442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_503536, whole genome shotgun sequence.
ACCESSION   DBNHOX010000651 DBNHOX010000000
VERSION     DBNHOX010000651.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_503536"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="AC16UZ_14305"
     CDS             <1..650
                     /locus_tag="AC16UZ_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247907.1"
                     /translation="DRVLESTARGQYTVGGGGAGLHRTVLSWADLEETLKPWAGEWLA
                     VYLGSLKRTNKTLVRARERALQLKGFVTVDPGLAVRPANLKASLPELLEDLRLLGLDP
                     SAGLYRIAETADGSRFAGLWDTDGLARRYQRHIDEIARSTRGIAELSPRAAAKETLLV
                     GRAAIRDILRDPLLPAELVDSRLRRELVHRMKKYDRLGKSCWRAFYADVGKADQR"
     gene            complement(641..1309)
                     /gene="queC"
                     /locus_tag="AC16UZ_14310"
     CDS             complement(641..1309)
                     /gene="queC"
                     /locus_tag="AC16UZ_14310"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HIR6247908.1"
                     /translation="MKRAIVLLSGGLDSATTLAVAKDEGFDCYALSIDYGQRHAAELA
                     AAREVARRAGVIEHRVIPVDVSVFGGSALTDRSIDVPEQPSEGIPITYVPARNTVFLS
                     LALAWAETLGLRDLFIGVNAVDYSGYPDCRPAFIFAFENLANLATKAGVEGDWYHVHA
                     PLIELSKAGIVELGISLGVDFGITVSCYQPDDDAAACGRCDSCRLRAQGFEEAGVEDP
                     TRYR"
     gene            complement(1306..1965)
                     /gene="queE"
                     /locus_tag="AC16UZ_14315"
     CDS             complement(1306..1965)
                     /gene="queE"
                     /locus_tag="AC16UZ_14315"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HIR6247909.1"
                     /translation="MTEQATRQLRISEIFLSLQGETSTVGLPTVFVRLTGCPLRCHYC
                     DTAYAFSGGEVMTLEDILARVRDLGVRRVTVTGGEPLAQPDCLGLLTSLCDEGFAVSL
                     ETSGALDVAGVDERVVKIIDVKTPGSGEVSRNRWENLAHCADGDQFKFVICHREDYEW
                     ARLKCEEHKLFEGACEVLFSPSSAELPARDLADWIVRDRLPVRMQIQLHKYLWGDQPG
                     R"
     gene            complement(1962..2720)
                     /gene="ybgF"
                     /locus_tag="AC16UZ_14320"
     CDS             complement(1962..2720)
                     /gene="ybgF"
                     /locus_tag="AC16UZ_14320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HIR6247910.1"
                     /translation="MTSPPKHRLRLVLKLALAGFLGSVVGPVAAEMTTETQSSAVPAP
                     AGDASEQRRLAELFYQLQVLRQEVQDLRGLVEEQSYQLNRLAKDQQEQYLDLDRRVAA
                     LRAQAPEPTLAETSPPPGGPVPGGVPGSERDAYTAAFNQMKNRQFDEAVASFNALVTD
                     YPNGQFTPNAFYWLGELHLAQADDEMARESFSQVINLYPDHQKAPDALYKLGIVHHRL
                     GDTANALQYLERVTAEYPQSSAAGLARTYSAEIR"
     gene            complement(2727..3170)
                     /gene="pal"
                     /locus_tag="AC16UZ_14325"
     CDS             complement(2727..3170)
                     /gene="pal"
                     /locus_tag="AC16UZ_14325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HIR6247911.1"
                     /translation="MAEPVETVDTTPPPAPPQPTPPPAFDPNGNPFAPGTQRLLSRTF
                     YFEYDRAVINPDDLASLELHASILRENPDRKIVIEGHCDERGTREYNLALGERRADAM
                     RSFLTSAGVSRRQIETVSYGEERPEDPGHDEGAWARNRRAIMIYR"
BASE COUNT          604 a         1146 c         1113 g          579 t
ORIGIN      
        1 gcgatcgggt tctcgagagc acggcgcggg ggcagtacac cgtgggcggg ggcggagccg
       61 gcctccaccg gaccgtgctc tcgtgggcgg atctggaaga aacgctcaaa ccctgggccg
      121 gggaatggct tgccgtctat ctcggcagcc tgaagcgcac caacaagacc ctggttcgcg
      181 cccgtgagcg agcattgcag ctcaaaggct tcgtcactgt cgacccaggg ctggcggttc
      241 gccccgccaa tctcaaagcc tccctgcccg agttgctcga ggacctgcgc ctgctcggtc
      301 tcgacccgtc cgccggtctc tatcgcatcg cggaaacggc agacggctcc cgctttgctg
      361 ggctttggga caccgacggt ctcgccaggc gctaccagcg ccacatcgac gagatcgccc
      421 gcagcacccg aggcatagcc gagctgtctc cccgcgcagc cgcgaaggaa acgctgctcg
      481 tcgggcgcgc cgccatcagg gacatcctcc gggacccgct gctgcccgcg gagctcgtcg
      541 acagccgtct gcggcgggaa ctggtgcacc ggatgaaaaa gtacgaccgt ctcgggaagt
      601 cctgctggcg ggcgttctac gcggacgtcg gcaaagccga tcagcggtaa cgggtcggat
      661 cctccacccc ggcttcctcg aatccctggg cacgaagacg gcagctgtcg cacctgccac
      721 atgcggctgc gtcatcgtcc ggctgataac aggacaccgt gatgccgaag tcgacgccga
      781 gcgagatccc cagctccacg atcccggctt tgctgagctc gatgagcggc gcgtgaacat
      841 gataccaatc gccctccacc ccggccttgg tggccaggtt ggcaaggttc tcgaaggcga
      901 agatgaaggc cggacggcaa tcgggatagc ctgagtaatc tacggcgttg acgccgatga
      961 aaaggtcgcg cagcccgagc gtttcagccc aagccagcgc tagcgagagg aacacggtgt
     1021 tccgggccgg cacgtaggtg atgggaatgc cctcgctggg ctgttcgggc acgtcgattg
     1081 atctgtcggt gagcgcagat ccgccgaaga cggaaacgtc caccgggatc acgcggtgct
     1141 cgatcacccc cgcccgtcgc gcgacttccc tggccgcagc gagctccgcc gcatgccgct
     1201 ggccgtagtc gatcgagagc gcgtagcagt cgaagccctc gtccttggcc acggcaagcg
     1261 tagtggcgga atccagcccg cccgacagga gcacgatggc gcgcttcatc ttcccggttg
     1321 atctccccac aggtatttgt gcagctggat ctgcatcctc accggaagcc ggtcccggac
     1381 gatccaatca gccaggtcac gggctggcag ctccgcgctg ctcggcgaga acagcacttc
     1441 gcaagcgcct tcgaacagct tgtgctcctc gcacttgagc ctcgcccact cgtagtcctc
     1501 acgatgacag atcacgaact tgaactgatc cccgtccgcg caatgcgcaa gattttccca
     1561 tcggttacgg gacacctcgc cggatcccgg cgttttgacg tcgatgatct tcacgacgcg
     1621 ctcgtccacg ccggccacgt ccagcgcacc gctggtctcc agagagaccg caaatccctc
     1681 gtcgcacagg gaggtgagaa gaccgagaca atcgggctga gccagcggtt ctccgccggt
     1741 caccgtgacc cggcggacac ccaggtcacg cacacgggcc aggatgtctt ccagagtcat
     1801 cacctcgccc ccgctgaagg cgtacgccgt gtcgcagtaa tggcaccgca gcggacaccc
     1861 ggtgaggcgg acgaagaccg tgggcaggcc aacggtgctg gtctccccct ggagggacag
     1921 aaaaatttcc gatatgcgaa gctggcgcgt tgcctgttcg gtcaccgaat ctccgccgag
     1981 taggtccggg cgaggccggc cgccgagctt tgcgggtact cggcggtcac gcgctcgaga
     2041 tactgcagcg cattcgccgt gtcaccgagc cggtggtgaa ctatgcccag cttgtagagg
     2101 gcatccggcg ccttctggtg atcggggtac aggttgatca cctgggagaa ggattccctc
     2161 gccatctcgt cgtcggcctg ggccagatga agctcgccaa gccagtaaaa agcgttgggc
     2221 gtgaactgac cattgggata atccgtgacc agcgcgttga acgacgccac cgcctcgtcg
     2281 aactgccggt tcttcatctg attgaaggcg gccgtgtaag catcgcgttc cgagccgggc
     2341 acgccgccgg gcaccgggcc gccgggtggc ggcgacgtct cggccagggt gggttcgggc
     2401 gcttgcgcgc gcagcgcggc cacccggcga tccagatcga ggtactgctc ctgctgatcc
     2461 ttggcgagcc ggttcagctg gtagctctgc tcttccacca ggccccggag atcctggacc
     2521 tcctgcctga gaacctgcag ctgatagaag agctcggcca gccggcgctg ctcgctggcg
     2581 tccccggccg gcgcaggcac ggcggaactc tgggtttcgg tcgtcatctc ggccgcgacc
     2641 gggccgacga ccgaacctag aaacccggcg agggccagct tcagcaccag ccgcagacgg
     2701 tgttttggcg gtgacgtcac cacggtctag cggtagatca tgatggcccg gcgattccgc
     2761 gcccaggcgc cctcgtcgtg gcccgggtcc tcggggcgct cttccccgta gctcacggtt
     2821 tcgatctggc gtcgagagac acccgccgag gtcagaaaac ttcgcatcgc gtccgcccgg
     2881 cgttcgccga gcgccaggtt gtactcccgg gtgccgcgct cgtcacaatg cccctcgatg
     2941 acgatcttgc ggtccgggtt ctcccttaag atggaagcgt gcagctccag ggaagccagg
     3001 tcgtccgggt tgatgaccgc acggtcgtac tcgaagtaga acgtccgcga caggagccgc
     3061 tgggttcccg gcgcgaaagg attgccgttc ggatcgaagg ccggcggcgg cgtaggctgg
     3121 ggtggggccg gcggcggggt cgtgtccacc gtctcgacag gctcggccac gggttccgct
     3181 ggagcgggct ggcctccggt tgcgcagccc gccagaaccg ccccgaaaaa gaaaatcgcg
     3241 aggagagtcc gaaaccaccg tgttgtcatc tgtatctcct gtgttgctgg ggttccacgg
     3301 ctgctgagaa ctgccaggct cggagcccgt tcagtccacc ctctccagaa catcgagata
     3361 aggggaccag gccggctccc gaacatcacc cgaggaagag ggcaatctgt acttcacgcg
     3421 gccgtcgatc gaaacgacgg cg
//
LOCUS       DBNHOX010000652         2806 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1724083, whole genome shotgun sequence.
ACCESSION   DBNHOX010000652 DBNHOX010000000
VERSION     DBNHOX010000652.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1724083"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="AC16UZ_14330"
     CDS             complement(<1..1249)
                     /locus_tag="AC16UZ_14330"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6247912.1"
                     /translation="MNVKPVPTLDEEVNEIRLATAEIVNEDILPNEDRLWGWLADSAN
                     ATNEVKQEAKALRVDIQEKVKNARLWAPHLPREFGGMGLTFMQHAYMNEVLAYSPGAA
                     SLFGVVAPNSGNQKILVKYGTPDQHEKWLKPLTDGTAQSGFSMTEPHNPGSDPRSITT
                     RAELDGDEWVINGHKWFTSNGFAADFYIVMCRTSDPDDPGGANEKMTQIIVPRKTPGV
                     NIVRGVPVWGRASSHCEIVYDNVRVPKENQLGRTGTGHQAAQDRLGAGRVYHCMNSIG
                     SMWRAFDLMIDRLMTREVHGGEKLEDKQFMQGFVADSYIDIQTARLMTIHCAEKMESG
                     ADPRTDISAIKIFVPQAYHRVVDRAIQVWGAAGVSGDLPLASMYQGARTLRLADGPDE
                     VHRILIAKNILRRYHAGESWDFGN"
     gene            1405..2514
                     /locus_tag="AC16UZ_14335"
     CDS             1405..2514
                     /locus_tag="AC16UZ_14335"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6247913.1"
                     /translation="MGTHELNYPHAEHPAPGTTVELAPGVRWLTMPMGGSLNHINLYL
                     LEDTTGWWIVDTGLSLDQTRELWHEIFSRELQGRPVLGVICTHFHPDHIGQSKMITDE
                     FRCSLYMTRAEYYQARAFSGSGPTYQSSWLGETFYQRAGMPADYLEQLREMWAHRSSE
                     GMSMPDMPAGYRRLEDGDVLTIGGHDWRIVVGSGHSPEHACLYCAALKIMISGDQILP
                     VITSNVSVHPTEPEANPLKDWMESHDRFLRVPDDTLVLPAHNLPFYGVRERLRQLIDH
                     HEDRMLAIEEHCVEPAVAKDLLPVLFARELDPRQMMMALGEAIAHLHLLVHRNRIERT
                     LHEDGCYRFLSIDPDLKRRAHPGDHDAPSERPVLV"
     gene            2540..>2806
                     /locus_tag="AC16UZ_14340"
     CDS             2540..>2806
                     /locus_tag="AC16UZ_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247914.1"
                     /translation="MQKGKLKRRIADYTYRMMHWGQNRLPPGIRSLVGVLLMVGGVFG
                     FLPVLGFWMLPLGVAFVALDVPGTRRHIDMWMLRLYRQANLDEPT"
BASE COUNT          491 a          940 c          844 g          531 t
ORIGIN      
        1 agttgccgaa atcccaggac tcgccggcat ggtatcggcg cagtatgttc ttggcgatga
       61 ggattcgatg gacctcgtcc ggtccatccg ccaggcgcag ggtgcgcgcg ccctgataca
      121 tgctcgccag cggcagatcg cccgacacgc ccgcagcgcc ccagacctga atcgcgcgat
      181 ccaccacccg gtggtaagcc tgaggcacga atatcttgat ggcggaaatg tccgtgcgcg
      241 gatccgctcc gctttccatc ttttcagcgc aatggatcgt catcagcctg gcggtctgaa
      301 tatcgatgta gctgtcggcg acgaagccct gcatgaactg tttgtcttcc agtttctcgc
      361 cgccgtggac ttcccgcgtc atgagacggt cgatcatcag gtcgaacgcg cgccacatgg
      421 agccgatgga gttcatgcag tgataaacgc ggcccgctcc gagccgatcc tgggccgcct
      481 gatgcccggt accggtgcgg ccgagctgat tctccttcgg cacgcgaacg ttgtcgtaga
      541 cgatctcgca gtgactggac gctcggcccc acaccggtac tccgcgcacg atgttcaccc
      601 cgggagtttt gcgcggcacg atgatctgcg tcatcttctc gttggcgccg ccgggatcgt
      661 ccggatccga cgtccggcac atcacgatat agaaatcggc cgcaaacccg ttcgaggtga
      721 accacttgtg cccattgatg acccattcat cgccatcgag ctccgcccgg gtcgtgatcg
      781 agcgtggatc tgatcccggg ttgtgcggct cggtcatgga aaagcccgac tgggcggtgc
      841 cgtccgtcag cggcttgagc catttctcgt gctgatccgg cgtgccgtac ttgaccagga
      901 tcttctggtt gccggaattc ggcgccacga ccccgaacag ggaagccgca ccggggctgt
      961 aggccagcac ctcgttcatg tacgcgtgct gcatgaaagt caggcccatg ccgccgaatt
     1021 cccgcggcag gtgcggcgcc cagagccggg cgttcttcac tttctcctga atgtcgacac
     1081 gcagcgcctt ggcttcttgc ttgacctcgt tggtcgcgtt ggcgctgtcg gctaaccaac
     1141 cccacaagcg gtcctcgttg ggcagaatgt cctcattgac gatctcggcg gtggccagcc
     1201 ggatctcgtt gacctcctcg tcgagcgtcg gtaccggctt cacattcatc gacacgacaa
     1261 ttcctccctc atgcggccag tcgattaatc taattgaaca cggcccgcat tgcgactgac
     1321 cggtacccgg gtgccggggc ccgctactgc agattgggct ttttggtatg ctgctggatc
     1381 gaggactgca aaccgggaac gacgatgggc actcacgagc tcaactatcc gcacgccgag
     1441 catccggcac cgggaaccac ggtcgagctc gccccgggcg tgcgctggct gaccatgccc
     1501 atgggcggct cgctgaacca catcaatctg tatctgctgg aagacacgac cggctggtgg
     1561 atagtggaca ccggcttgag cctcgaccag acccgcgagc tgtggcacga gatcttctcc
     1621 cgagagctcc agggcaggcc cgtcctgggc gtcatctgca cccactttca tccggaccac
     1681 atcggccagt cgaagatgat caccgacgag ttccgctgca gcctctacat gacccgcgcc
     1741 gagtactacc aggcccgcgc cttttcgggc agcgggccga cctatcagtc cagctggctg
     1801 ggcgagacgt tctaccagcg cgccggcatg cccgcagact acctcgagca actccgcgag
     1861 atgtgggcgc accggtcttc ggaaggcatg tcgatgcccg acatgccggc cggctaccgg
     1921 cgcctggagg acggggacgt tctcaccatc ggcggtcacg actggcgcat cgtggtgggc
     1981 tcgggccact cacctgaaca tgcctgcctg tactgcgcgg ccctgaagat catgatctcg
     2041 ggcgaccaga tcctgccagt gatcacgagc aacgtcagcg tgcaccccac cgagccagag
     2101 gcgaacccgc tgaaggactg gatggaatcc cacgaccgat tcctgcgggt ccccgacgac
     2161 accctcgtcc tgccggctca caacctgccg ttttacggcg ttcgggagcg gctgcgccag
     2221 ctgatcgacc accacgagga tcgcatgctg gccatcgagg aacactgcgt cgagcccgcg
     2281 gtggcgaagg acctgcttcc cgttctattc gcacgcgagc tcgacccccg tcagatgatg
     2341 atggccctgg gcgaagccat cgcccatctg cacctgctgg tgcatcgcaa ccgcatcgag
     2401 cggacgctgc acgaggacgg ctgctaccgc ttcctgtcca tcgacccgga cctgaaacgg
     2461 cgagcgcatc ccggggacca cgatgccccg agcgagcggc ccgtgctggt ctgagccgga
     2521 agagcggagc cacacggcat tgcagaaggg caaactcaaa cggcgaattg ccgactacac
     2581 ctaccgcatg atgcattggg gtcagaaccg cctgcccccg gggatccgct ctctggtggg
     2641 cgtgctctta atggtcggcg gcgttttcgg ttttctaccc gtgctcggtt tctggatgct
     2701 gccgctgggc gtcgccttcg tcgccctcga cgttcccggc acgcgacgcc acatcgacat
     2761 gtggatgctg cgcctgtacc gccaggccaa cctggacgag cctacg
//
LOCUS       DBNHOX010000653         6543 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1080764, whole genome shotgun sequence.
ACCESSION   DBNHOX010000653 DBNHOX010000000
VERSION     DBNHOX010000653.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1080764"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..127
                     /locus_tag="AC16UZ_14345"
     CDS             <1..127
                     /locus_tag="AC16UZ_14345"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR6247915.1"
                     /translation="TWFRVTVGPFDSQLKANRALTQLREIDLDAFMIQQRLPEPR"
     gene            238..780
                     /gene="hslV"
                     /locus_tag="AC16UZ_14350"
     CDS             238..780
                     /gene="hslV"
                     /locus_tag="AC16UZ_14350"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014754676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIR6247916.1"
                     /translation="MADIHGTTIVSVRNDRQVAIGGDGQVSMGQTIMKGNARKVRRLH
                     DDKVLAGFAGGTADAFTLFERFEGVLDKYHGNLTRAAVELAKDWRSDRALRRLEALLV
                     VADREATLLVSGTGDVIEPEAGVIAIGSGGPFAQAAARALNENTELDPADIVEKSLSI
                     AADICVYTNHHRTIEVLDLT"
     gene            786..2129
                     /gene="hslU"
                     /locus_tag="AC16UZ_14355"
     CDS             786..2129
                     /gene="hslU"
                     /locus_tag="AC16UZ_14355"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019650789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIR6247917.1"
                     /translation="MTPREIVHELDKHIIGQDDAKRAVAIALRNRWRRMQLEEEIREE
                     ISPKNILMIGPTGVGKTEIARRLARLANAPFIKVEATKFTEVGYVGRDVESIIRDLAD
                     VAVKMLREEQMDRVMHQAEDRAEERILDALLPGPKPTSSQTIGFTQESSEDEGDSTTR
                     QLFRKRLRQGELDEKEIEIETDAAQVGVEIMAPPGMEEMTSQLQSMFSNLGGGRKRSV
                     RLKIKDALRKLKEEEAAKLVNEDEIKARAVETVEQMGIVFLDELDKVAKRSESAGADV
                     SREGVQRDLLPLIEGCTVSTKYGMVKTDHILFIASGAFHLAKPSDLIPELQGRLPIRV
                     ELAALSAGDFKRILVEPNASLTEQYQALLGIDGLSLEFTDDGIERIAELAWQVNESTE
                     NIGARRLHTVLERLLENVSFTATESAESTVVIDAVYVDAQLSELAQDRDLSRYIL"
     gene            2146..2559
                     /locus_tag="AC16UZ_14360"
     CDS             2146..2559
                     /locus_tag="AC16UZ_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase family protein"
                     /protein_id="HIR6247918.1"
                     /translation="MQRPKQITYRRQSKVLVLSFEDGDAEIPAELLRVYSPSAEVRGH
                     SAAERKLQTGKKHVGIKSLEPVGNYAIRITFDDGHDTGLYGWDLLRDLAENADGYWKS
                     YLDELKAANASRLPTIPVGHWTPASQPAVPPEQDQ"
     gene            2556..3299
                     /locus_tag="AC16UZ_14365"
     CDS             2556..3299
                     /locus_tag="AC16UZ_14365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008096122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6247919.1"
                     /translation="MSDQNSVDFGSRKVSPDEKTRLVGGVFASVAERYDVMNDLISLG
                     THRLFKRMTVELSGVREGMRVLDLAGGTGDLSALFSGAVGPSGVVVLADINARMMDVG
                     RNRLLDQGCANVSFCQAAAEALPFADDSFDCVSIGFGLRNFTDKRVALEELNRVIAPG
                     GVLLILEFSRPENAFLEGAYGTFQSLWPGLGKLVAGDARSYQYLIESIRNHPPQKALK
                     LMLEDAGFAAVEYHNLLGGIAAIHRGVKP"
     gene            3296..3934
                     /locus_tag="AC16UZ_14370"
     CDS             3296..3934
                     /locus_tag="AC16UZ_14370"
                     /inference="COORDINATES: protein motif:HMM:NF014132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HIR6247920.1"
                     /translation="MSTADVFESALVELVNRAANATLELDPKAASRLAAAEGHRARLI
                     AALPGGREQSFTLSVVGGRMAFTPHAEAEPNVVVRGSVPALLSMLAGGRSAGPVTIEG
                     DVSVLEELAAIFKGFAPDLAEPLSKLVGETSADGILNAAEAGLALLKSAAEATGESLR
                     RGVGERFATRTRVNVLLDRLEDARERSDRLAARIGNLESARGSHQRPEPPTP"
     gene            3931..5595
                     /gene="ubiB"
                     /locus_tag="AC16UZ_14375"
     CDS             3931..5595
                     /gene="ubiB"
                     /locus_tag="AC16UZ_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HIR6247921.1"
                     /translation="MSALRRLFRVLWIVSRYRLDTLIDADQEGAALPRRYAWLLRASP
                     ARLLPVGDEPRGRRLLLALEQLGPVFVKFGQLLSTRRDLIPLDIADELSALQDRVPPF
                     PGEQAVRIIEDALGQPISEAFRSFEIEPMASASVAQVHAAILPDGKSVVVKVVRPDIA
                     DVIRADLELLRAAAEILENASEEARRLHLKQVVKDYEQTILGELDMQKEADNTARLRS
                     NFAESELLYVPRVYWRWCRSNILVIERIHGIPISDVAELKARGTNMRRLAEKGVETFF
                     KQVFVDNFFHADMHPGNIFVDVTDPEDPRYIAVDCAIIGSLNQEDQDYLARNLLAFFN
                     RDYAEVARLHLESGWVPDETDPEEFERVIREVCEPIFAKPLNEISFGQFLVELFETAS
                     AFHMEIQPQLVLLQKTLLYIEGLGRELYPELDLWETAKPFMESWMAERVGPAAALREL
                     AERAPEILAELPRLPGMVVTASRQIRHLERMVDRQNRELHELEARVGRLARGVRGRRV
                     AGAALLVLASVLLWGPIAESLSGSGDLGTVAGLASAAIASLLLLKA"
     gene            5599..5946
                     /locus_tag="AC16UZ_14380"
     CDS             5599..5946
                     /locus_tag="AC16UZ_14380"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HIR6247922.1"
                     /translation="MTDSKDIVEDLIEVLESRRSADPGSSYVASLYADGLNRILEKVG
                     EEATEVVLAAKDATSSDRDDALVHEVADLWFHTLVMLVHLNQDPRWVFETLRERFGIS
                     GHEEKSSRNAGGP"
     gene            6024..6257
                     /gene="tatA"
                     /locus_tag="AC16UZ_14385"
     CDS             6024..6257
                     /gene="tatA"
                     /locus_tag="AC16UZ_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR6247923.1"
                     /translation="MFGLGMTELIVILIIVVLIFGPKRLKTIGSDLGNAIKGFRKAVT
                     EEEAKAAPAEEPRVIEGEVNAAQKTETKQDSSV"
     gene            6260..>6543
                     /gene="tatB"
                     /locus_tag="AC16UZ_14390"
     CDS             6260..>6543
                     /gene="tatB"
                     /locus_tag="AC16UZ_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HIR6247924.1"
                     /translation="MFDIGFPELVLIAVVALLVIGPERLPEALRTLGLWLGRMRRSFT
                     AVKTEIEKEIGMDDVRRQLHNEAIMEEMKRIEDEVRGTADSPAGDAQTEAA"
BASE COUNT         1255 a         2070 c         2148 g         1070 t
ORIGIN      
        1 cacctggttc cgggtcaccg tcggcccgtt cgacagccag ctgaaggcca atcgagcgct
       61 tacgcagctt cgcgaaatcg atctggacgc gttcatgatc cagcagcgcc tgcccgaacc
      121 ccggtgacca aaccccgttg gcggaagtcc gttggcggaa ccccgatggc ggaaccccgt
      181 tgaaatgccg ggcagagggc cccactatga ccagttcgca atcggttgaa gagctaaatg
      241 gcggatatcc acgggacgac catcgtcagc gtgcgcaacg acaggcaggt ggccattggc
      301 ggagacggtc aggtctccat gggccagacc atcatgaagg gcaatgcccg caaggtgcgg
      361 cgcctgcatg acgacaaggt cctggctggt ttcgccggcg gaaccgccga cgccttcacc
      421 cttttcgagc gcttcgaggg cgtcctcgac aagtatcacg gcaacctcac ccgtgccgcc
      481 gtggagctgg ccaaggactg gcggtcggac cgcgcccttc gacgtctcga ggcccttctg
      541 gtggtggcgg accgggaggc cacgctcctg gtgagcggga ccggcgacgt catcgagccg
      601 gaagccggcg tgatcgccat cggctccggc ggcccctttg cccaggcggc agcgcgcgcc
      661 ctgaacgaga acacggaact ggatcccgcc gacatcgtcg agaaatcgct gtccatcgcc
      721 gcggacatct gcgtctacac caaccatcac cggaccatcg aggtgctgga tctgacatga
      781 cccagatgac gccgagagag atcgttcacg agctcgataa gcacatcatc ggccaggacg
      841 atgccaaacg ggccgtggcc atcgcgctga gaaaccgctg gcggcggatg cagctcgaag
      901 aggagatacg cgaggagata tcgcccaaaa acatcctgat gatcgggccc accggggtgg
      961 gcaaaaccga gattgcgcgg cggctcgccc gcctggcgaa tgccccgttc atcaaggtgg
     1021 aagccaccaa gttcaccgag gtgggatacg tgggtcggga tgtcgagtcg atcatccggg
     1081 atctggcaga cgtggcggtg aagatgctgc gcgaggagca gatggaccgc gtcatgcacc
     1141 aggccgagga tcgggccgaa gagcgcatcc tggacgcgct gctccccgga ccgaagccca
     1201 ccagctctca gaccatcggc ttcacccagg aatcctcgga ggacgaaggg gactccacca
     1261 cgcgtcagct cttccgcaag cgcctgcgac agggcgagct cgacgaaaag gaaatcgaga
     1321 tcgagacgga tgcggcccag gtcggcgtgg agatcatggc gcctcccggc atggaggaga
     1381 tgaccagcca gctgcagagc atgttcagca acctcggcgg cggcagaaaa cgctccgtgc
     1441 gcctgaaaat caaggacgcg ttgcgcaagc tcaaggaaga ggaagcggcg aagctggtaa
     1501 acgaggacga gatcaaggca cgcgccgtgg aaaccgtgga gcagatgggc atcgtgttcc
     1561 tggacgagct ggacaaggtg gcaaagcgca gcgagagcgc gggcgcggac gtctcccggg
     1621 agggcgtgca gcgcgacctg ctccccctga tcgagggctg cacggtgtcc accaagtacg
     1681 gcatggtgaa aaccgatcac atcctcttca tcgccagcgg cgcgtttcac ctggcgaaac
     1741 cttccgatct gatccccgag ctgcagggcc ggctgcccat ccgcgtagag cttgcggccc
     1801 tgtctgccgg cgacttcaag cgaatcctgg tcgagcccaa cgcatcgctc accgagcagt
     1861 atcaggcgct gctggggatc gatgggctca gcctcgaatt caccgacgat ggcatcgagc
     1921 gcatcgccga gctggcgtgg caggtcaacg agagcaccga gaacatcggc gcgcggcgcc
     1981 tgcacacggt cctggagcgc ctcctggaaa acgtttcgtt cacggctacc gagagcgcgg
     2041 aaagcacggt ggtcatcgac gcggtctacg tggacgctca gctctcggag ctggcccagg
     2101 accgcgatct ttccagatac atcctctagg cgacgggcca aggccatgca gcgcccaaag
     2161 cagattacct atcgccgcca gtcaaaagtc ctggttctct ctttcgagga cggcgacgcg
     2221 gagatccccg cggagctgct gcgcgtctac tcaccctctg cggaggtgcg gggccactcg
     2281 gccgcagagc gcaagctgca gacgggcaag aagcacgtcg gcatcaaaag cctggagccc
     2341 gtaggtaatt acgcgatccg cattaccttc gatgacggcc acgacaccgg tctgtacggt
     2401 tgggaccttc tgcgggatct cgccgagaac gccgacggtt actggaagtc ctatctcgac
     2461 gagctgaagg cggccaacgc ctcgcggctt cccaccatac cggtgggcca ctggacgccg
     2521 gcaagccagc cggccgtccc cccggagcag gatcaatgag cgatcagaac tccgtcgact
     2581 tcggctcccg caaggtatcg ccagatgaga agacccgcct ggtaggcgga gttttcgcga
     2641 gcgtggctga acgctacgac gtcatgaacg acctgatctc tctgggcacc caccgcctgt
     2701 tcaagcggat gacggtggag ctctcgggag tccgggaggg catgcgcgtg ctggacctgg
     2761 ccggcggcac cggcgatctg agcgcgttgt tctctggtgc ggttggcccg tcgggagttg
     2821 tcgtgctcgc ggacatcaac gcacgcatga tggacgtggg ccgcaaccgt ctgctggacc
     2881 agggttgcgc aaacgtctcg ttctgccagg cagcggcgga agctctgccc ttcgccgacg
     2941 acagcttcga ctgcgtgagc atcgggttcg gattgcgtaa cttcaccgac aaacgggttg
     3001 cgctcgagga gctcaatcgc gtgatcgcgc cgggcggcgt gctgctgatc ctggagttct
     3061 ccaggccgga gaacgcattt ctggaggggg cctacgggac gtttcagtcg ctctggcccg
     3121 gactcggcaa gctggtggct ggcgacgccc gaagctacca gtacctcatc gagtcgattc
     3181 gcaatcatcc cccgcagaag gcgctcaagc tcatgctcga agacgccggc ttcgccgccg
     3241 tcgagtatca caatctgctg gggggcatcg cggccattca ccgcggggtc aagccatgag
     3301 caccgcagac gttttcgaat cagccctggt ggagctcgtc aacagggcgg ccaacgcaac
     3361 gctcgagctg gaccccaaag ccgcatcccg gctggccgcg gcggaaggac accgggcgcg
     3421 cctcatcgca gcgctgcccg gcggccgtga gcaatccttc acgctgtcgg tcgtcggggg
     3481 gcgcatggcg ttcacgccgc acgccgaggc cgagcccaac gtagtcgtgc gcggttccgt
     3541 tccggctctg ctttccatgc tcgccggagg cagaagcgcc gggccggtca ccatcgaagg
     3601 cgacgtcagc gtgctggagg agctggcggc catcttcaag ggcttcgcac ccgacctggc
     3661 cgagccgctg tcgaagctgg ttggagaaac ctcagcagac ggcattctga acgcggcgga
     3721 ggcaggtctg gccctgctga aatctgcagc agaggctaca ggcgaatccc tgcggcgcgg
     3781 cgtgggcgag cgttttgcga cgcgcacccg cgtcaacgtt cttctcgatc ggctggaaga
     3841 tgcccgcgaa cgatcggacc gcctcgccgc acgcataggc aatctggaga gcgcccgggg
     3901 ctcccaccag cgcccggaac ctccaacgcc atgagcgctc tgcgccgact ctttcgggtt
     3961 ctctggatcg tgagccgcta ccggctcgac accctgatcg acgcggatca ggaaggcgcg
     4021 gcgttgccgc gtcgctacgc ctggctactg cgcgcttctc ccgcccggct tctaccggtc
     4081 ggcgacgaac cgcgcgggcg gcgcctgctg ctggcgctgg agcagctcgg gccggtgttc
     4141 gtcaaattcg gacagctgct ttccacgcgg cgggatctga taccgctgga cattgcggac
     4201 gagctcagcg ccctgcagga tcgggtgccc ccgtttcccg gcgagcaggc cgtgcggatc
     4261 atcgaggacg cgctgggcca acccatcagc gaagccttcc gctccttcga gatagagccc
     4321 atggcatccg cgtctgtggc ccaggtgcat gccgccatcc tgccggacgg caagagcgtg
     4381 gtggtcaagg tcgtcagacc cgacatagcc gacgtcatcc gggcagatct ggaactgctg
     4441 cgcgccgccg cggagattct ggagaacgcc tctgaggagg cgcggcggct gcacctcaag
     4501 caggtggtca aggactacga gcaaacgatt ctcggtgagc tcgacatgca gaaggaggcg
     4561 gacaacaccg cccggctgcg cagcaacttc gccgagtcgg agctcctgta cgtgccgcgc
     4621 gtctactggc gctggtgccg cagcaacatc ctggtgatcg agcgtatcca cggaataccg
     4681 atctccgatg tggccgagct caaagcccgc gggacgaaca tgcggcgcct ggccgagaaa
     4741 ggggtggaga cctttttcaa gcaggtgttc gtggacaact tcttccacgc ggacatgcac
     4801 ccgggcaaca tcttcgtcga cgtgaccgat cccgaggacc cgcgctacat tgccgtggac
     4861 tgcgcgatca tcggttcgct caaccaggag gatcaggact acctcgcccg aaatcttctg
     4921 gcgttcttca accgggatta cgccgaggtc gcgcggctgc atctggagtc cggctgggtt
     4981 cccgacgaaa cggaccccga agagttcgag cgggtcattc gcgaggtctg cgagcccatc
     5041 ttcgcaaaac cgctcaacga gatctctttc ggtcagtttc tcgtggagct cttcgaaacg
     5101 gccagcgcct ttcacatgga gattcagcct cagctggtgc tgctgcagaa gacgcttctg
     5161 tacatcgaag gcctgggtcg ggagctgtac ccggagctgg atctctggga gacggccaaa
     5221 ccgttcatgg aatcctggat ggccgagcgg gtgggaccgg cggccgccct gcgggagctg
     5281 gcagaacgcg cgccggagat tctcgcggag ctgccgcgcc tgcctggcat ggtggtgacg
     5341 gccagccggc agatacgcca cctcgaaagg atggtggacc gacagaaccg ggaactgcac
     5401 gagctcgagg cgcgcgtggg caggctcgcc cggggcgtgc gcggacgccg ggtggcgggc
     5461 gccgctttgc tcgtgctggc cagcgtccta ctatggggcc ctatcgctga atcgctgagc
     5521 ggcagcggag atctcggcac cgtggctggt ctcgccagcg cggccatcgc ttcgttgctg
     5581 ctcttgaagg cctgaaacat gaccgatagc aaagacatcg tcgaagacct gatcgaagtt
     5641 ctcgaatccc gccgcagcgc cgaccccggt tcgtcctacg tcgcatcgct gtacgctgac
     5701 ggcctgaaca ggattctcga gaaagtgggc gaggaagcca ccgaggtggt gctcgctgcc
     5761 aaggatgcga cgtcgtccga cagggacgat gcgctcgttc acgaggtggc cgatctgtgg
     5821 tttcacactc tggtcatgct cgtgcatctc aatcaggacc cacgctgggt tttcgaaacg
     5881 ttgcgagagc gtttcggcat ctccgggcac gaggagaaaa gcagccgcaa cgccggcggc
     5941 ccctgacggg cctattggat tccgacgagg tgggctgggt tcggaagacg tatacttgac
     6001 caacgacctg acggagacag caaatgtttg gactgggcat gaccgaactc atcgtcatcc
     6061 tcatcatcgt ggtcctcata ttcggcccca agcggctgaa aaccatcggc tctgacctgg
     6121 gaaacgccat caaggggttt cgcaaagccg tgaccgagga agaggccaag gccgcgcccg
     6181 cggaggagcc ccgcgtgatc gaaggtgagg tcaacgcggc gcagaagact gagaccaagc
     6241 aggactccag cgtctagcaa tgttcgacat cggctttccg gagctcgttc tcatcgccgt
     6301 ggtcgcgctg ctggtcatcg gaccggagcg gctgcccgaa gcgctgcgga ccctaggact
     6361 gtggctcggc agaatgcgcc gcagcttcac cgctgtgaag acggagatcg agaaggagat
     6421 cggcatggac gacgtgcgcc gccaactcca caacgaggcc atcatggagg agatgaagcg
     6481 catcgaggac gaggtgcgtg gaaccgccga cagccccgcc ggcgatgctc agaccgaggc
     6541 tgc
//
LOCUS       DBNHOX010000654         2227 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_504055, whole genome shotgun sequence.
ACCESSION   DBNHOX010000654 DBNHOX010000000
VERSION     DBNHOX010000654.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_504055"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="AC16UZ_14395"
     CDS             complement(<1..1060)
                     /locus_tag="AC16UZ_14395"
                     /inference="COORDINATES: protein motif:HMM:NF020120.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6247925.1"
                     /translation="MLGAPTRLRCEYLSNPLGIDETRPRLSWWVDDDRPAELQTAYRI
                     LAASSPQRLAAEEGDLWDSGRVASHQTLNVEYHGGPLTTGQRVWWSVRSFDSDGLPSP
                     WSEPAFFEVGPCGQEDWSARWIGASLMGGAATSVQVPVFRRGFELPEAALAARLHVAA
                     LGVCAVEINGQRVGRDELTPGWTDFRKRVRYRTYDVTDHLQTGENALGALLGDGWYCG
                     HPGAGQRQQYGDRPLLLAQLNITLASGATIAIGTDEAWKWRPSWLLQADVLGGESVDA
                     RQRIGDWSSPGCADDGWDPAQVHDLPDLTVCASMHPPIRAQGVLEPVADAERRDDPVR
                     PPRWIFDFGQNLLGRVLVS"
     gene            complement(1169..2005)
                     /locus_tag="AC16UZ_14400"
     CDS             complement(1169..2005)
                     /locus_tag="AC16UZ_14400"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6247926.1"
                     /translation="MNWEDLRYFDAVARLGSVRRAASALGVNPSTVTRRVEQLERHLG
                     VRLFTRSRRGLELTADAAVAAGTLRELADRLRSVEDQLGQQTGALGGEVRCGIPDYLP
                     TGWWMPEIARFTGEHPELMVAFMESNRLPDLASGEADLAVNARKDPPEALIARPLGQL
                     VFAAYRHLDCHDEAWLGSALQAAIAPDYPGGARVTAILPSVSLQVAAVEAGMGSSLLP
                     CAAVDDRPGFVRDESRPEVIRPLWLLLHPDSRPLARVQTVADALTDALRARLARSAGN
                     ER"
BASE COUNT          388 a          762 c          725 g          352 t
ORIGIN      
        1 cgctgaccag cacccggccg agcaggttct gcccgaaatc gaatatccag cgcggcggcc
       61 gaaccgggtc gtcacggcgc tcagcatcgg ccacgggctc caggaccccc tgggcacgga
      121 tgggggggtg catgctggcg cacacggtga ggtccggcag atcgtgcacc tgggccggat
      181 cccagccgtc atccgcgcag cccggtgagc tccagtcccc gatgcgctga cgggcgtcga
      241 cgctttcgcc gccgagcacg tccgcctgca gcaaccagga cgggcgccat ttccaggctt
      301 cgtcggtgcc gatggcgatc gtcgccccgg aggccagggt gatgttcagc tgggccagca
      361 gcagcggtcg gtcgccgtat tgctgccgtt gcccggcccc gggatgcccg cagtaccagc
      421 cgtcccccag cagcgcgcca agggcatttt ccccagtctg gagatggtcg gtcacgtcgt
      481 aagtgcggta gcgaacccgt ttgcggaaat ccgtccagcc cggcgtgagc tcgtcacgtc
      541 cgacacgttg cccgttgatc tcgacggcac agacgccgag ggcggccacg tgcaaccgag
      601 cggcgagcgc tgcctcggga agctcgaaac cgcgcctgaa gacgggaacc tgaacgctgg
      661 tggccgcgcc gcccatgagc gaggcgccga tccaacgagc gctccagtcc tcctgcccgc
      721 aggggccgac ctcgaagaaa gcgggttcgc tccaggggct cggcagaccg tcgctgtcga
      781 acgagcgcac cgaccaccac acgcgctgac cagtggtaag cgggccgccg tggtactcca
      841 cgttcagggt ctgatggctg gccacccgac ccgagtccca aagatcgccc tcttccgcag
      901 ctagccgctg cgggctgctc gcggcaagaa tacggtacgc ggtctggagt tcggcgggcc
      961 ggtcgtcgtc cacccaccag gaaaggcggg gtcgggtctc gtcaatgcca agcggattgg
     1021 aaaggtactc gcagcgaagc ctcgtcggcg cgcctagcat tgcttctcct tcagcagagg
     1081 aaacgcgtcc ctgacgggcg gccattctgc gctgcgcggc cgagccgcgc aagccggggt
     1141 gcccgaacgg atgaaaggcc atccgtgatc atcgctcatt gccggcagat cgggccaagc
     1201 gggcgcggag ggcatcagtg agcgcatcgg cgacggtctg aacccgggca aggggtctcg
     1261 agtccgggtg caacagcagc cacagcggcc tgatcacttc gggccggctc tcgtcgcgga
     1321 cgaaccccgg gcggtcatcg accgcagcgc aaggcagcag gctgctgccc atgcccgcct
     1381 ccacggcagc cacctgcagc gacacggacg gcaggatcgc ggtgacccga gcaccaccgg
     1441 ggtaatcggg cgcaatggct gcttggagtg ccgagccgag ccaggcctcg tcatggcagt
     1501 cgaggtgacg gtaggctgca aacaccagtt ggccaagcgg ccgggcaatg agggcctccg
     1561 ggggatcctt ccgggcattc accgccaggt cggcttcacc ggacgcgagg tccggcagtc
     1621 ggttggactc catgaacgcc accatgagct cgggatgttc cccggtgaac ctcgctatct
     1681 ccggcatcca ccagccggtc ggcaggtagt cgggaatgcc gcaacggacc tcacccccca
     1741 gggctccggt ttgctgcccg agctgatcct cgacggatcg aagccgatcg gccaattctc
     1801 tgagggttcc ggcagcaacg gcggcgtcgg cggtcagctc cagtccacga cgtgagcggg
     1861 tgaacaggcg cacacccaga tgcctttcaa gctgctccac ccgtctggtg accgtggacg
     1921 ggttcacgcc aagcgccgac gcagcccggc gaaccgatcc caaccgggcc acggcatcga
     1981 agtatctgag gtcttcccag ttcaccatgg gggatccttt ggatttctcg cccgccagcg
     2041 ttgcgattat gcaacattat gttgcgttac ccaaggttgc attctgtgtt accgaaagta
     2101 acataggttc gacgattcgc tgaggggtac ccgaaatccc tcccctcccc tcccttcggg
     2161 tatccctccg tttcgacccc tccctgtctt aaacggcggg tgcagtgact aaccaagcac
     2221 tgacccg
//
LOCUS       DBNHOX010000655         1518 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1402930, whole genome shotgun sequence.
ACCESSION   DBNHOX010000655 DBNHOX010000000
VERSION     DBNHOX010000655.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1402930"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="AC16UZ_14405"
     CDS             <1..1482
                     /locus_tag="AC16UZ_14405"
                     /inference="COORDINATES: protein motif:HMM:NF015926.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HIR6247927.1"
                     /translation="STAASAIDEGAVRAATEALGRARAILQALPARGREAQARALQQE
                     SARLSELRDWQTFATTPKREALCEAVQNLADQPLDPPDQAERIKALRGEWNGLGPVSG
                     REDRALADRFNRLAEQAFEPCRAYFAEQASVRASNLVERGKICDQLENYLESTDWKAA
                     DIKAAERILRAARDEWRRFQPVDRNPGKPVEERFEVLQERLHKLIKAEWDANLQLKRQ
                     IVEEAEALAGSEADPHDRAQEAKALQRRWREIGITPRGPDRKLWQQFRGACDAIFDAL
                     GAAREASHQAVSEALDAADNLLTSFESDLGNTSATEAEAEILTGFRRRFSELPPLPER
                     EGRQISRRFEELTRSYRSLLHQKSRYQERAELDRLRALDEAVTHTEIAPGLTAEPTQD
                     AIADPVVSERLQKSGEPIPMDALRALTVRAEILAGAESPAQDREIRLKLQVEEMNVGM
                     GSQRRAQDPLEMAREWCARGPKDEACSALRERFFDALARALED"
BASE COUNT          288 a          505 c          527 g          198 t
ORIGIN      
        1 agcaccgcgg cgagcgccat cgatgagggc gccgttcgag cggccacgga ggccctcggc
       61 cgcgcccggg caattctgca ggcccttccc gcgcgtggga gggaagccca ggccagagcc
      121 cttcagcagg agtcggccag gctgtccgag ctgcgtgact ggcagacttt cgcgaccacg
      181 ccgaagcggg aggccctttg cgaagccgtt cagaacctcg ccgatcagcc cctggatccg
      241 ccggatcagg ccgagcgaat caaagcgctg cgcggcgagt ggaacggtct cgggcccgtg
      301 tccggacgcg aagatcgcgc cctcgccgat cgtttcaacc gcctcgccga gcaggcattc
      361 gagccgtgcc gagcctactt cgccgagcag gccagcgtac gcgcaagcaa cctggtcgag
      421 cgcgggaaga tctgcgatca gctggaaaac tacctggaaa gcaccgactg gaaagcggcg
      481 gacatcaagg cggccgagag aattctgcgc gccgcccggg atgagtggcg cagattccaa
      541 cccgtcgacc ggaacccggg caaacccgtc gaggagcggt tcgaggtgct ccaggaacgg
      601 ctccacaagc tcatcaaagc cgagtgggat gccaacctgc agctgaaacg gcagatcgtc
      661 gaggaggcgg aagcacttgc cggcagcgaa gccgaccccc acgaccgggc ccaggaggca
      721 aaagcgctgc aacggcgctg gcgggagata ggcatcacgc ccagaggccc cgaccgaaag
      781 ctgtggcagc agtttcgcgg cgcgtgtgat gcgatcttcg atgcgctggg agccgcccgg
      841 gaggcatccc accaggcggt cagcgaggcc ctcgacgctg ctgataacct gctgacctcc
      901 tttgagagcg accttgggaa tacgtcagca accgaggccg aggcagagat cctcaccggc
      961 ttccgacgcc gcttcagcga gctgccgcca ctgccggagc gggaggggcg gcagatttcc
     1021 cggcgcttcg aggagttgac ccgcagctac cgatcccttc tgcatcagaa gtcccgctac
     1081 caggagcggg ccgagctcga tcgcctcaga gccctggacg aggcggtgac ccacacggag
     1141 atcgcgccgg gcctgacggc ggagccgacg caagatgcta tcgcggatcc cgtcgtctcc
     1201 gagcgtctgc agaaaagcgg cgaaccgatc cccatggacg cccttcgcgc gctcaccgtg
     1261 cgggcggaga tcctcgccgg cgcggaatcc ccggcccagg accgggagat tcgcttgaag
     1321 cttcaggtcg aggagatgaa cgtcgggatg ggcagccagc ggcgtgctca agaccctctg
     1381 gagatggccc gcgagtggtg cgcccgcggt cccaaagacg aggcgtgctc ggcgctgcgc
     1441 gagcggttct tcgacgcgct cgcacgggcg ctggaggact aggcgggcag gtccaggcag
     1501 atgcgggcgc cccccagg
//
LOCUS       DBNHOX010000656         2483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1338758, whole genome shotgun sequence.
ACCESSION   DBNHOX010000656 DBNHOX010000000
VERSION     DBNHOX010000656.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1338758"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="AC16UZ_14410"
     CDS             complement(<1..283)
                     /locus_tag="AC16UZ_14410"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6247928.1"
                     /translation="MDTNGLRRDPEGARRRILDSAAEHFSRHGPDGARVDAIAAAARV
                     NKRMLYHYFGDKQALFRAVLDDRAARPGGTGNTSGWDTTTARLLVWAAGL"
     gene            513..1058
                     /locus_tag="AC16UZ_14415"
     CDS             513..1058
                     /locus_tag="AC16UZ_14415"
                     /inference="COORDINATES: protein motif:HMM:NF016269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HIR6247929.1"
                     /translation="MRSFLRFLTLCAVCLAPAGAAWSASKVEIDARISAALESLYEIE
                     PAARELAAKASGVLVMPRVYKLGFGLGGEYGEGALLINNQAVQYYRVTAASFGFQLGG
                     QAKSEVLLFMSSEALDGFRNSDGWEAGVDGAITFIEFGVGKDFDTHTLQDPIIGFVYG
                     NKGLMYDLSLEGSKFWRIDKN"
     gene            complement(1068..1634)
                     /locus_tag="AC16UZ_14420"
     CDS             complement(1068..1634)
                     /locus_tag="AC16UZ_14420"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR6247930.1"
                     /translation="MYYTYMDGPVGRLLLAGDGKRLKLIGFSTGARARGAQQDWERYD
                     EPFRPAKRQLDEYFDGRRRYFDIELAPDVTPFQGKVLEALLEIPYGETRSYGEIARAI
                     GQPRAVRAVGGANGSNPIPIVIPCHRVIGSNGSLTGFGGGLDTKQYLLDLERSHSGLF
                     EGLVGDSQQAPQRPVRAGKGQVVFTVTR"
     gene            complement(1658..>2483)
                     /locus_tag="AC16UZ_14425"
     CDS             complement(1658..>2483)
                     /locus_tag="AC16UZ_14425"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF017809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR6247931.1"
                     /translation="REPFDAPWMFRFLEQRALPGLEEVHGLEYRRRVGSGESWITVSQ
                     RGGALMLKVPAGTSASLSSILRRVRRLFDLQADSAVIDAHLARDPGLAPLVAASPGLR
                     VPGAWDGFETAVRAILGQQVSVERARRLAETLIHRCGEGEFPKPRALARFVPAAIGIP
                     GKRWLAIAELSRAVADGVIDLDEGAARERLTDRLLALPGVGPWTAGYIGMRVAKDPDA
                     FVDGDWAVLKALQATPRAARRLAEPWRPWRAYAVMHLWSAPVPSVDAVRLQSVSGT"
BASE COUNT          435 a          848 c          784 g          416 t
ORIGIN      
        1 ccagaccggc tgcccagacc agcaggcgcg ccgtcgtggt atcccagccg gacgtgttcc
       61 ctgtgccgcc aggccgcgcc gcgcgatcgt ccaggaccgc ccggaaaagc gcctgcttgt
      121 caccaaagta gtgatagagc atccgcttgt tgacacgggc ggcggccgcg atggcgtcca
      181 cccgggcacc gtccgggcca tggcgcgaga agtgctcggc agccgaatcc agaattcgcc
      241 ggcgagctcc ctcggggtca cgcctgaggc cgtttgtatc catttggtta ctcaatactg
      301 ccagtcagcg gctgaaaaga tcatatgtat ccatttagtt ataacttcgc ctgggctccg
      361 tcagcgaacg ttcggccggt atctggatca ttcgcttgcc ggcccttcgc gacaggtata
      421 gtaaacaatt ggttacataa tttctgccct cgaccaaggc tccgcccggt ggtataaaac
      481 gccccggtct caaccgagca ggatgatgcc cgatgcgttc gttccttcga ttcctcaccc
      541 tttgcgccgt ctgccttgca cctgccgggg ccgcatggtc tgcctccaag gtcgagatcg
      601 acgcccgcat ctccgccgcc cttgaatcgc tctacgagat cgagcccgcc gcgcgggaac
      661 tggcggcaaa agcctccggc gtgctggtga tgccgcgcgt ctacaagctc gggttcgggc
      721 tgggcggcga gtacggcgag ggcgcgctgc tgatcaacaa tcaggcggtg cagtactacc
      781 gggtgacggc cgcgtcgttc ggtttccagc tcggcggcca ggccaaatcc gaggtcttgc
      841 tgttcatgtc atccgaagcg ctggacggct tccgcaacag tgacggctgg gaggccggcg
      901 tggacggggc catcaccttc atcgagttcg gcgtcggcaa ggatttcgac acccacaccc
      961 tgcaggaccc gatcatcggc ttcgtctacg gcaacaaggg cctgatgtac gatctttcgc
     1021 tggaaggctc caagttctgg aggatcgaca agaactgagc gagccgtcta gcgggtgacc
     1081 gtgaacacca cctgcccttt gccggcacgc acgggtcgtt ggggcgcctg ctggctgtca
     1141 ccgacgagac cctcgaagag tccggagtgg ctgcgctcca ggtcgaggag gtactgcttg
     1201 gtgtccaggc caccgccaaa gcccgtgagc gagccgttgc tgccgatgac cctgtggcag
     1261 gggatcacga tagggatcgg attgctgccg ttggccccgc cgacggcccg caccgcgcgg
     1321 ggctgaccga tggcccgcgc aatctcgccg taagaacgtg tctccccata cgggatctcg
     1381 agcagcgcct ccagcacctt gccctgaaac ggggtcacgt cgggcgcaag ctcgatgtcg
     1441 aagtagcggc gccggccgtc gaagtactcg tcgagctggc gtttcgccgg gcgaaacggc
     1501 tcgtcgtagc gttcccagtc ctgctgcgca ccgcgcgccc gggcgcccgt ggagaatccg
     1561 atcagcttca ggcgcttgcc gtcaccggcg agcagcagtc ggcccaccgg gccgtccata
     1621 taggtgtagt acatgtgccc ttccccgtcg ttgcgggtca cgtccccgac accgattgta
     1681 accggaccgc atcgaccgac ggcacgggag cagaccagag atgcatgacc gcgtacgccc
     1741 gccagggtcg ccacggctcg gccaagcggc gcgcagctct cggtgtggcc tgcaacgcct
     1801 tgagcaccgc ccagtcaccg tcaacgaacg catcgggatc cttcgcgacc cgcatgccga
     1861 tgtagccggc cgtccacgga cccaccccgg gtagagccag cagtcggtcc gtcagccgtt
     1921 cacgagctgc accctcgtcg agatcgatga caccatccgc caccgcccgg gaaagctcgg
     1981 caatggcaag ccaacgcttg cccggaattc caatggcggc cggcacgaag cgcgccaggg
     2041 cgcggggctt cgggaactcc ccttccccgc agcggtggat cagggtctcg gcaaggcgtc
     2101 tggcccgttc cacgctgacc tgttgtccca gtatggcgcg aacggcggtt tcgaagccgt
     2161 cccaggcacc cggcaccctc aatcccggcg acgcggcaac gaggggcgca aggcccggat
     2221 cccgcgcgag gtgcgcgtcg atgacggcag agtccgcctg gaggtcgaac agccgcctga
     2281 cgcgccgaag tatcgaggac agcgacgccg aagtgcccgc gggcactttc agcatcaacg
     2341 cgcccccgcg ctgggatacc gtgatccagc tttccccgga tccgacccgc cggcgatact
     2401 cgagcccgtg cacctcctcg agaccgggca acgcgcgttg ctcgagaaag cggaacatcc
     2461 acggcgcatc gaaaggctca cgg
//
LOCUS       DBNHOX010000657         2535 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1017884, whole genome shotgun sequence.
ACCESSION   DBNHOX010000657 DBNHOX010000000
VERSION     DBNHOX010000657.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1017884"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="AC16UZ_14430"
     CDS             <1..204
                     /locus_tag="AC16UZ_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HIR6247932.1"
                     /translation="PDSELPSRGRTIELERQVAPRFIRGDDYGTRASTAVIIGADGIR
                     FAEQEFGPQGVPGRRSDFEVARA"
     gene            complement(213..2498)
                     /gene="ptsP"
                     /locus_tag="AC16UZ_14435"
     CDS             complement(213..2498)
                     /gene="ptsP"
                     /locus_tag="AC16UZ_14435"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009396724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR6247933.1"
                     /translation="MLTTLRRIVHGVEESSSFKEALSLLVGEVREALGTEVCSAYLLN
                     PEGDSYVLVANEGLNTDAVDKLSLGLNQGLVTLVAERAEPINLSDATHHPRFYLVPEI
                     GEEAFNAFLGVPVIANRRVLGVLVVQERDARRFDESEEAFLVTLAAQLATVIAHHEAT
                     GEIQQLLEDSSVEPIAREDALYKGVPGAPGISLGTAVVMHPTASLEAVPERIAEDVRT
                     ELMVLDRAVNAVRDDIRRISEQFAQSLPPEELALFDAYLHMLDDNALAGDVRDRINRG
                     QWAQGALKQVIREHVRRFEAMEDPYLRERGADVRDLGMRVLAYLQDIRSRKTYFPDDT
                     ILVGHEITPSMLANIPPEKLKGIVSVRGSSNSHVAILARALGIPAVMGAMDLPIFSME
                     NISVVVDGFYGEVFTNPSLALAGQYLQLIAEEREFAEELEDLRELPSVTKDGWPVGLW
                     VNIGLTGDITRSLDRGAEGIGLFRTEVPFMEKERFPSEEEQRAIYREHMEAFDPRPVT
                     MRTLDVGGDKSLPYFPIHEDNPFLGWRGIRVTLDHPEIFLVQVRAMIKANAGLECLLR
                     IMLPMVSSLSEVEEAKALVERAYVEVIEEGIVVKQPLVGVMIEVPAAVYQARQLARMV
                     DFLAVGSNDLTQYMLAVDRNNPRVAQLYKEMHPAVLQALREVARAAHAEEKVVGICGE
                     LAGTPAGAVLLMAMGYDALSMNATNLPKVKWTLRNVKRSDARRMLVNVLHMDTAEEIQ
                     QFMQDWLIDSGLGRVVPSYHH"
BASE COUNT          438 a          826 c          822 g          449 t
ORIGIN      
        1 ccggattcgg agctgccgag ccgggggcgc acgatcgagc tcgaacgaca ggtagccccg
       61 agattcatcc ggggtgatga ctacggaacc agagcgagca cggccgtcat catcggcgcg
      121 gacggcatcc gcttcgccga gcaggagttt ggtccccagg gcgtgcccgg caggcgctcg
      181 gacttcgagg tcgcgcgtgc gtgaggtgcg tgtcagtgat ggtagctggg aacgacccgg
      241 cccaggcccg agtcgatcag ccagtcctgc atgaactgct gaatctcctc agcggtatcc
      301 atgtgcagga cgttgaccag catgcgacgg gcatctgagc gtttgacgtt gcgcagggtc
      361 catttcactt tcggcaggtt ggtggcgttc atggacagcg cgtcgtagcc catcgccatc
      421 agcagcaccg ctccggccgg ggtgcccgcc agctctccgc agatgcccac gaccttctct
      481 tcggcgtgag ccgcccgggc cacctcccga agcgcttgca gaaccgccgg atgcatctcc
      541 ttgtaaagct gggcgacccg tgggttgttg cggtcgacgg caagcatgta ctgggtcaga
      601 tcgttcgatc ctactgccag gaagtccacc atacgggcca gctggcgcgc ctggtatacg
      661 gcggcgggca cctcgatcat cacccccacc aggggttgct tcaccacgat cccctcttcg
      721 atgacctcca cgtaggccct ctccaccagc gccttggctt cctcgacctc ggagagcgag
      781 gagaccatgg gcagcatgat ccgcagcagg cattccaggc cggcgttcgc cttgatcatg
      841 gcgcgaacct gcaccaggaa gatttccggg tggtccagcg tgacccggat gccgcgccag
      901 ccgaggaaag ggttgtcctc gtggatgggg aagtagggca gcgatttgtc cccacccaca
      961 tccagcgtgc gcatggtcac ggggcgcgga tcgaaagcct ccatgtgttc ccggtaaatg
     1021 gcacgctgct cctcctcgct ggggaagcgc tccttctcca tgaagggcac ctcggtgcgg
     1081 aacaggccga tcccctccgc gccgcggtcc agagagcggg tgatgtcacc ggtgagcccg
     1141 atgttgaccc aaagccccac cggccagccg tccttggtga cgctgggcag ctcccgcagg
     1201 tcctcgagct cctcggcgaa ctcgcgctct tccgcgatga gctggagata ctggccggcc
     1261 agggccagcg aagggttggt gaaaacctcg ccgtagaaac cgtctacgac gacggaaatg
     1321 ttctccatgc tgaagatcgg cagatccatg gcccccatga ccgccgggat gcccagggcc
     1381 cgcgccagaa tcgccacgtg ggagttgctc gagcctcgca cggatacgat gcccttgagc
     1441 ttttccggtg ggatgttggc cagcatgctg ggggtgattt cgtggccgac caggatggtg
     1501 tcgtcgggga aataggtctt ccgcgagcgg atgtcctgaa gataggcgag cacccgcata
     1561 cccagatccc tgacatcggc tccgcgctcg cgcagataag ggtcctccat ggcttcgaag
     1621 cgccggacgt gctcgcgaat cacctgcttg agggcgcctt gtgcccactg tccacgattg
     1681 atgcgatcgc gcacgtcacc ggcgagcgcg ttgtcgtcga gcatgtggag gtaggcatcg
     1741 aagagcgcaa gctcctcggg aggcaggctc tgagcgaact gctcgcttat cctccggatg
     1801 tcgtcgcgca ccgcgttgac ggctcggtcg agcaccatga gctcggtgcg aacatcctcg
     1861 gcaatgcgtt cgggcaccgc ttccaggctc gccgtcgggt gcatgacgac cgcggtgcca
     1921 agactgattc ccggagcacc agggacgccc ttgtaaagag cgtcctcccg ggcgatgggc
     1981 tcgaccgagc tgtcctccag gagctgctga atctcgccgg tggcctcgtg gtgagcgatc
     2041 accgtcgcca gctgggcggc gagcgtgacc agaaaggcct cctcgctctc gtcgaaccgg
     2101 cgcgcatcgc gctcctggac caccagcacc ccgagcaccc ggcggttggc aatgacgggc
     2161 acgccgagga acgcgttgaa cgcctcctcg ccgatctccg gcaccaggta aaagcgggga
     2221 tggtgggtgg cgtcgctgag gttgatgggt tccgcccgtt ccgcgaccag cgtcaccaat
     2281 ccctggttca ggccgaggga aagcttgtcg accgcgtcgg tattgagccc ctcgttggcc
     2341 accagcacgt agctgtcgcc ttcggggttg agcaggtaag cggagcagac ctcggtgccg
     2401 agcgcctctc gaacttcgcc caccagcagg ctcagcgcct ccttgaacga gctggattcc
     2461 tcgactccat ggacgatccg ccgcagcgtg gtcagcatgg gctggccaca tcctcaacgg
     2521 gcagatgcgg cgcaa
//
LOCUS       DBNHOX010000658         3103 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1273735, whole genome shotgun sequence.
ACCESSION   DBNHOX010000658 DBNHOX010000000
VERSION     DBNHOX010000658.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1273735"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="AC16UZ_14440"
     CDS             complement(<1..823)
                     /locus_tag="AC16UZ_14440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HIR6247934.1"
                     /translation="MIARTGVLLVSMLWAASAKAEPVGFAACVSNLESVAVASGISEA
                     TAKQVLGEVRHLERVIELDRTQPEFVRTFSEYYRVRVTPSRVADGRRLLEENAALLGR
                     LQRQYGIPGHYLIALWGLETNFGSYFGNMSTMDSLATLACDERRSAFFTAELMDALRI
                     VDAGDMEPAAMQGSWAGAMGHVQFMPSVFRRYAVDDDGDGRRDLWTSRRDALASAANY
                     LKQAGWERGFRWGREVRLPEGFDYAGAARDPTRPLADWEAAGVRNAFGGPLASTDL"
     gene            complement(831..2201)
                     /locus_tag="AC16UZ_14445"
     CDS             complement(831..2201)
                     /locus_tag="AC16UZ_14445"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6247935.1"
                     /translation="MTQPRLSKATLFCYGVADLPVMLAIMPMSIWLSRFYTGDMGLSL
                     SAVANIMLLARLFDVFTDPLVGHLSDHTRTRWGRRKPWMLASLPLLMIGLYKVFLPPD
                     GIGIWYLFSWMMVMWLGWTMLMIPYYAWAAELSDDYDERTRITGWRAVMGSVGGIGAQ
                     LIPFIALVLFGFGGTANVMTMLGIAALILMPLCVGVALLRVPEFPEMRAPSVPILAGI
                     RIMWRNGPFRRLLLAFVLSSTGLAIVMPLYIFFVEFIVEEPPANVPYMIIISSVAGFL
                     GIPFWVWLSKHVGKHKAWIGGFLVVAAVSPMYLFLGPGDFWLMVPGIIIIGIGTGSFS
                     ALPNSMKADVIDLDTARSGKNRAAFFFSAWSLVTKLASSLGGWLALQSLALFGFDAAN
                     GAQNTPEALTGLRLTFAVLPAIIFVIAAAVIWRYPITKERQARIRAAIDRRTLRRNAA
                     QAGFAK"
     gene            complement(2394..>3103)
                     /locus_tag="AC16UZ_14450"
     CDS             complement(2394..>3103)
                     /locus_tag="AC16UZ_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247936.1"
                     /translation="QQPVQVFACNFKDGKNLDNLAAWIEDFREFATSGENQDPGSGSF
                     AWFPFRGAAPYDYILGFISSSLNDMATAVQGYYASPEGRALEAKANETSDCISAIVMS
                     EQLRNGVIGNTGDDQLDAVVELFSCTLNKGSDLDDVRSDASFLNKQIDAIGSAALSQY
                     EAYLWSPYRGGTGSWDFLWVGNYPNLSTWAQGDTDYYASKEGMAAEERFQKTSTCTNS
                     IWGGYWIVPPAEGPTAQ"
BASE COUNT          569 a          993 c         1040 g          501 t
ORIGIN      
        1 gcagatccgt ggacgccagc gggcctccga acgcgttgcg caccccggcg gcctcccaat
       61 ccgcgagggg ccgcgtgggg tcacgggcgg cgcccgcgta gtcgaacccc tcgggcaggc
      121 gtacctcgcg cccccagcgg aaaccccgct cccagccagc ctgtttgagg taattggccg
      181 cagatgccag ggcgtcgcgc cggctggtcc agagatccct tctgccgtcg ccgtcgtcgt
      241 ccacggcgta gcggcggaag accgaaggca tgaactgaac gtggcccatg gcgcccgccc
      301 aggagccctg catggctgcg ggctccatgt ccccggcgtc gacgatgcgc agggcgtcca
      361 tcagctccgc cgtgaagaag gcgctgcggc gctcgtcgca ggccagcgtg gcgagcgagt
      421 ccatggtgct catgttgccg aagtagctgc cgaagttggt ctccagaccc cacagcgcga
      481 tcaggtagtg gccgggaatc ccgtactggc gctgcagccg gccgagaagg gcggcattct
      541 cctcgagcag gcggcgcccg tcggccaccc tcgatggggt cacccgcacc cggtagtact
      601 cgctgaacgt gcgaacgaac tccggctgcg tgcggtcgag ctcgatcacc cgctccaggt
      661 ggcgtacctc gccgagtacc tgcttcgcgg tcgcctcgga aatcccggac gccacggcaa
      721 cggattccag gttgctgacg caggccgcga accccacggg ctcggcttta gccgacgcgg
      781 cccagagcat ggatacgagc agcacgcccg ttcgcgcgat caccgcggcg ttacttcgcg
      841 aagcctgctt gggctgcgtt tcggcgcagc gtgcgccggt cgatggctgc ccggatgcgg
      901 gcctggcgct ccttggtgat cgggtagcgc cagatcacgg cggcagctat cacgaagatg
      961 atggccggca gcacggcgaa ggtgagccgc agcccggtca gcgcctccgg cgtgttctgc
     1021 gcaccgttgg ccgcgtcgaa gccgaacagg gccagagact gcagagccag ccagccgcca
     1081 aggctcgagg ccagcttggt caccagggac cacgcggaga agaagaaggc ggcccggttc
     1141 ttgccgctgc gtgccgtgtc caggtcgatc acgtcggcct tcatggagtt gggcagggcg
     1201 ctgaaggacc cggtgccgat accgatgatg atgatgccgg gcaccatcag ccagaagtcg
     1261 ccggggccga ggaagagata catggggctc accgcggcta ccaccagaaa gccgccaatc
     1321 cacgctttgt gcttccctac gtgcttggag agccagaccc agaacggtat gcccagaaaa
     1381 ccggctaccg agctgatgat gatcatgtag gggacgttgg caggcggctc ctcgacgatg
     1441 aactcgacga agaagatgta cagcggcatg acgatggcga gcccggtgga ggacaggacg
     1501 aaagcgagta acaggcgacg gaacggaccg ttgcgccaca tgatccggat gcccgccaag
     1561 atgggcaccg acggcgcccg catctccgga aactcgggca cccgaagcag cgccacgccc
     1621 acgcacaggg gcatgagaat gagcgccgcg atacctagca tggtcatgac gttggcggtg
     1681 ccgccgaagc cgaacagaac cagcgcgatg aaggggatca gctgggcgcc gatgcctccc
     1741 acgctgccca tgaccgcgcg ccagccggtg atgcgcgtgc gctcgtcgta gtcgtcggag
     1801 agctcggcgg cccaggcgta gtagggaatc atcagcatgg tccagccgag ccacatgacc
     1861 atcatccacg agaacaggta ccagatgccg atgccgtcgg gcggcagaaa caccttgtac
     1921 aggccgatca tgagcaatgg cagcgacgcc agcatccagg gcttgcgcct gccccatcgg
     1981 gtgcgggtgt gatcgctcaa gtgccccacc agggggtcgg tgaacacgtc gaagagcctg
     2041 gccagcagca tgatgttggc caccgccgac aggctcaggc ccatgtcgcc ggtgtagaag
     2101 cgcgacagcc agatggacat gggcatgatc gccagcatca ccggaagatc ggcgacgccg
     2161 tagcagaaca gggtggcctt gctgagccgc ggctgggtca actgcgttcc cgattggcgg
     2221 gcttggaggc ttcagtgtaa gtcggccgcg cctgaggaat ccaccatgac tcggcgcgct
     2281 ctgggtgctc ggcgatcggc cgctggtcgt caatcccgtc gtgacggttc ccaagccggc
     2341 gacggcgtca tgccgaccct ggtgtgcttc cagggccggc atggtggagt accctattgg
     2401 gcggtgggtc cctcggcggg cggtacgatc cagtagccgc cccagatgga gttggtgcag
     2461 gtgctggtct tctggaaccg ttcctcggcc gccatgccct ccttagaggc atagtaatcg
     2521 gtgtctccct gggcccaggt cgagaggttg ggatagttgc cgacccagag gaaatcccag
     2581 ctgccggtcc cgcctcgata cggactccag aggtacgcct cgtactgact cagcgcggcg
     2641 gagccgatgg cgtcgatctg cttgttcaaa aagctggcgt cgctgcgcac gtcatcgaga
     2701 tcggatcctt tgttcagagt gcaggagaac agctcgacca cggcgtccag ctggtcgtcg
     2761 cctgtattcc cgatgacccc gttgcgaagc tgctcgctca tgacgatggc ggagatgcag
     2821 tctgacgttt cattggcctt ggcttccaat gcccgaccct cgggcgacgc gtagtacccc
     2881 tgcacggcgg tagccatgtc gttcaggctg gagctgatga atccgagaat gtagtcgtat
     2941 ggcgcggcac cgcggaatgg gaaccatgcg aacgagcccg acccgggatc ctggttctct
     3001 cccgaagtgg cgaactcacg aaaatcctcg atccaggcag ccagattgtc gaggttcttg
     3061 ccgtccttga aattgcaggc gaacacctgc accggctgct gct
//
LOCUS       DBNHOX010000659         1817 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1466475, whole genome shotgun sequence.
ACCESSION   DBNHOX010000659 DBNHOX010000000
VERSION     DBNHOX010000659.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1466475"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="AC16UZ_14455"
     CDS             complement(<1..1122)
                     /locus_tag="AC16UZ_14455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012612.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR6247937.1"
                     /translation="MVASVAIASYAQACEICRKAYFDAMETAPKASALHPEARVVEYR
                     LTIAEQTLSPADRKVQALTINGGTPGPVLRLREGDVARITVVNGLADDSTSIHWHGLL
                     VPNIEDGVPVVTTPVIGPGQSRTFEFLVRQHGTYWYHSHTGHQEQRGVYGAIVLEPKT
                     ETKAADHDEVVILSDWTDEDPRDVQRSLLRGSDWYSFRKGTSQSLLGAYQAGKLGEYL
                     DREKALVPPMDLSDVAYDAFLMNGRRRLELPGKPGETVRVRLINAGAASYFYLDAATG
                     PLRIVAADGQDVKPFDQKRLRIGPAETYDVLVTGPAQGAWELRSTAQDGSGSVSAWVG
                     SGERHEATPPPAPDRYGMAAYLTSILDQIDPEPGAKEQER"
     gene            1278..1517
                     /locus_tag="AC16UZ_14460"
     CDS             1278..1517
                     /locus_tag="AC16UZ_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247938.1"
                     /translation="MSKDSIDELLSAWEVSPRRREAFSSQVIAGRPRGPMVRVSAAVL
                     AVFLVGGLAGWRANTDDTRQMAKAHARSIVEASFR"
     gene            1514..>1817
                     /locus_tag="AC16UZ_14465"
     CDS             1514..>1817
                     /locus_tag="AC16UZ_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247939.1"
                     /translation="MRTRLLLTIVLASLLAGYAGWWVARDFRKAEACERGSELGWLQA
                     EFRLDDTAVARIGQLQRRFEEECEVHCEQVRQARALMATKPGDESKVRLEAALGKCQ"
BASE COUNT          273 a          556 c          660 g          328 t
ORIGIN      
        1 gcgttcctgc tccttggcgc cgggttccgg gtcgatctgg tcgagtatgg aggtcaggta
       61 ggcggccatg ccgtaacgat cgggcgccgg cgggggcgtg gcttcgtgcc gctcacccga
      121 gccgacccag gccgagaccg agccgctgcc gtcctgggcc gtggatcgga gctcccatgc
      181 gccttgggcg ggtccggtga cgagcacgtc ataggtctcg gccggaccga tccgcagccg
      241 cttctggtcg aagggcttca cgtcctgccc gtcggcggcg acgatgcgca acgggccggt
      301 cgcggcgtcc agatagaagt aagaagccgc gccggcgttg atcaggcgga cgcggacggt
      361 ctcgccaggc ttgccgggaa gctccaggcg acgccggccg ttcatcagga acgcgtcgta
      421 ggccacgtcg gaaaggtcca tcggcggaac gagcgccttc tcgcggtcga ggtattcgcc
      481 gagcttgccg gcttggtaag cgccgaggag cgattgggac gtgcccttgc ggaacgaata
      541 ccagtcgctg ccgcgaagga gtgagcgctg cacgtcgcgc gggtcctcgt cggtccagtc
      601 ggagaggatg acgacctcgt cgtgatcggc ggccttcgtc tcggtcttgg gctctaggac
      661 gatggcgccg tacacgcctc gctgctcctg atgtcccgtg tggctgtggt accaataggt
      721 gccgtgttgc cgcacgagga actcgaaggt gcgggactgg cccgggccga tgaccggggt
      781 ggtcacgacc ggcacgccat cctcgatgtt gggcaccagc aggccgtgcc agtggataga
      841 ggtggagtcg tcggctagcc cgttgaccac cgtgatgcgt gcgacgtcgc cctcgcgtag
      901 ccgaagcacc gggccggggg tgcctccgtt gatggtcagg gcctggacct tgcggtcagc
      961 tgggctgagc gtctgctcgg cgatcgtgag gcggtactcg accacccggg cctcggggtg
     1021 cagtgcagag gccttgggcg ctgtctccat ggcgtcgaag taggccttac ggcagatttc
     1081 gcaggcctgc gcgtaggacg ctatggcgac tgaggcgacc atgagtcccc aggtcgaggt
     1141 ccttccggct ttgcgagctt ggtgggcgag catgcaagta catgtccgcg tcccccggca
     1201 tcccccccct gatgaaatac cccgtgccaa aaagatttag gggggacagg gccgggtccg
     1261 gacactagaa aggtaccatg agcaaggatt ccatcgacga gttgttgtcc gcttgggagg
     1321 tttccccccg taggcgcgag gctttttcca gccaggtcat cgcaggtcgg ccgcgtggcc
     1381 cgatggtccg cgtctccgcc gccgtgctgg cggtgttcct cgtcggcggc ttggccggct
     1441 ggcgggccaa tacggatgac acccgtcaga tggcgaaggc ccacgcccgg tcgatcgtcg
     1501 aggcttcctt ccgatgagga cgcgactgct gctgaccatc gtcctggcct cgttgttggc
     1561 aggttatgcc ggatggtggg ttgcgcgtga cttcaggaag gccgaggcct gcgagcgcgg
     1621 ctccgagctc ggctggctcc aggccgaatt caggctagac gatacggctg tcgccaggat
     1681 cgggcagctc cagcgtcgtt tcgaggagga gtgcgaggtg cactgcgagc aggttcgcca
     1741 ggctcgtgcc ctgatggcga cgaagcccgg cgacgaatct aaggtccggc tcgaggccgc
     1801 tctaggcaag tgccagg
//
LOCUS       DBNHOX010000660         3560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_695886, whole genome shotgun sequence.
ACCESSION   DBNHOX010000660 DBNHOX010000000
VERSION     DBNHOX010000660.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_695886"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /gene="hisF"
                     /locus_tag="AC16UZ_14470"
     CDS             <1..540
                     /gene="hisF"
                     /locus_tag="AC16UZ_14470"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF013168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR6247940.1"
                     /translation="GGVSSVGDATDLRLAGAEKINVNSAAVRRPQLIAECAEAIGRQN
                     VVLSMDIRRVEPCAELPSGYEIVIEGGRRPMAIDALEWALEGERLGAGELVINSIDAD
                     GTRDGYELTLTAMLAEAVRIPVIASGGAGKPEHLYDALTVGKADAALVASMVHYGDYK
                     VSELKRYLDGRGVKMRMSW"
     gene            589..1551
                     /locus_tag="AC16UZ_14475"
     CDS             589..1551
                     /locus_tag="AC16UZ_14475"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017255625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HIR6247941.1"
                     /translation="MKAVIIEGERLSWQEAEEPQAGVGEVLIAVHATAVNRADLVQRS
                     GGYPPPPGASKILGLECAGEVLAIGEGVTRVKPGDRVCALLAGGGYAERVAVPAGQVL
                     PVPSGLDMSQAAALPEVFATAYLNLYTEARLERGERVLIHAGASGVGTAAIQLCSNTS
                     NPCFVTVGNPQKIERCVALGAEGGWDRHQGSFLEAVRQWGGVDVVLDPVGAGYLADNL
                     SCLNIDGRLSLIGLMGGVQSEINLATVVMKRIRIIGSTLRARSAAAKSLVMDGLQRDV
                     WPHLEAGSIVPIIDEVLPIESAERAHELMAGDQTFGKLVLQIVE"
     gene            complement(1534..>3560)
                     /locus_tag="AC16UZ_14480"
     CDS             complement(1534..>3560)
                     /locus_tag="AC16UZ_14480"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR6247942.1"
                     /translation="LADREIPIVADDYVDPEFGSGCVKITPAHDFNDNEVGARHGLAL
                     INVFNEDATINEAAPQPYRGLDRFDAREKVVADLKSQGLLDGIEDHTLMVPRGDRSNA
                     IVEPLLTHQWFVKIQPLADPAIKAVENGDVEFVPKQYENVYFSWMRNIQDWAISRQQW
                     WGHRIPAWYDAEGNVYVGRDEDEARSNGGLADDVPLDQDPDVLDTWFSSALWTFVTLG
                     WPEETEELSQFHPTDVLVTGHDIIFFWVARMIMMTLKFTGEVPFRQVYVHGLVRDADG
                     KKMSKTKGNGLDPLDLIDGVSLDELIDKRTQNLTQPQMAPSIEKNTRADFPEGIPSYG
                     TDALRFTYCALASTGRDVRFDLSRIEGYRNFCNKLWNAARFVLMNCPDDEEPGRRDLS
                     LADRWIVSEMRSFLETSERAINTYRFDLYANAVYEFVWHEYCDWYLELTKPLLWDEQA
                     DPSLLRGTRYTLLSVLDTLLRAAHPIMPFITETIWREVAPRLGNTNPTIMLQPFPEPA
                     DLPSDPDAEAAVEWLKGVIVGIRNIRGEAGIKPAQEISILLQGGSDADRALAQVAESL
                     LKRLAKVDRIDWLADGAEPPPNALALVGELRVMVPLAGLIDVAAERARLAKEVEKKSQ
                     ELNRIEAKLANENFVAKAPQAVVEKERAKAGDARAALATLEQQLASLDDL"
BASE COUNT          573 a         1096 c         1228 g          663 t
ORIGIN      
        1 ggcggggtca gctcggtggg ggatgccacg gatctgcgcc tggccggggc ggaaaaaatc
       61 aacgtcaact cggcggccgt gcggcgcccg cagctcatcg ccgagtgcgc ggaggccatc
      121 gggcgtcaga acgtggtctt gtccatggac atccgacgtg tggaaccctg cgcggagctg
      181 ccttccggtt acgagatcgt gatagagggc ggccgtcggc ccatggccat agacgctctg
      241 gagtgggccc tggaagggga gcggctcggc gcgggcgagc tggtgatcaa ctccatcgat
      301 gccgacggga cccgggacgg ctacgagctc accctcactg ccatgctggc ggaggccgtg
      361 cgaattcctg ttatcgcctc gggtggtgcg ggcaagcccg agcaccttta cgatgcgctg
      421 accgtgggga aagcggacgc tgcgctggtg gcttccatgg tgcactacgg cgattacaag
      481 gtgagcgagc tgaagcggta cctcgacggc cgcggggtca agatgcggat gtcctggtga
      541 gcatcagcgt gggcgagcga aaaaggccat taccagtcag ggagagcaat gaaagccgtc
      601 atcattgaag gagagcgtct gagctggcag gaggccgaag agcctcaggc cggggtcggt
      661 gaggtgctga tcgccgtgca cgcgacggcc gtcaatcgtg cggacctggt gcagcgctcg
      721 ggaggctacc cgcccccacc gggagcgagc aagatcctgg gcctggaatg cgcgggagag
      781 gtgctggcca ttggggaagg cgtgacccgg gtaaagcccg gcgatcgggt ctgcgcgctc
      841 ctggccgggg gcggctacgc ggagcgcgtc gcggtccctg cggggcaggt cctgccggtg
      901 ccgtccgggc tggacatgtc gcaggcggcc gccctgccgg aggtatttgc caccgcttat
      961 ctcaaccttt acacggaggc gcgtctggag cgcggtgagc gcgtgctcat ccatgccggg
     1021 gcaagcggcg tcggtaccgc ggccattcag ctgtgcagca acacgagcaa tccctgcttc
     1081 gtaaccgtgg gcaatcctca gaagatcgaa cgctgcgtgg ctttgggggc cgagggcggc
     1141 tgggatcggc accagggcag tttcctcgaa gccgtcaggc agtggggcgg cgtcgacgtc
     1201 gtgctcgatc cggtcggagc cggctacctt gcagacaacc tttcctgtct caacatagat
     1261 ggccggctct cgctgatcgg tttgatgggt ggggttcagt ccgagatcaa cctcgccacc
     1321 gtggtcatga agcgaatccg catcatcggc tccacgctca gggcgcgctc ggcggcggcc
     1381 aagtcgctgg tcatggacgg tctgcagcgg gacgtctggc cgcatcttga agcggggtcc
     1441 atcgttccga taatcgacga ggtgctcccc atcgaatccg cggagcgggc tcacgagctc
     1501 atggcgggcg atcagacgtt cggtaagctg gtgttacaga tcgtcgagtg aggcgagctg
     1561 ctgctcgagc gtggccagcg cggcgcgtgc atcgccggct ttggcccgct ccttttccac
     1621 caccgcctgc ggggccttgg cgacgaaatt ctcgttggcc agcttcgcct cgatgcggtt
     1681 gagctcctgg gactttttct ccacttcctt ggcgagccgc gcgcgttccg cagccacgtc
     1741 gatcaggccg gccaggggga ccatcactct cagctcgccg acgagcgcca gagcgtttgg
     1801 tggcggttcc gcgccgtcgg ccagccagtc tatgcggtcg acctttgcca gccgcttgag
     1861 cagcgattcc gccacctgag cgagcgcccg atccgcgtcg ctgccgccct gcagaagaat
     1921 gctgatctcc tgcgctggct tgatgccggc ttctccgcgg atgttgcgga tgccgacgat
     1981 gacgcccttt agccactcga cggccgcttc cgcgtccgga tccgacggca ggtccgcagg
     2041 ctccggaaac ggttgcagca tgatggtcgg gttggtgttg cccagccgcg gggccacctc
     2101 gcgccagatg gtctcggtga tgaacggcat gatcgggtgg gctgcgcgca gcagcgtgtc
     2161 cagcacgctc agcagcgtgt agcgcgtccc gcgcaggagg ctcgggtcgg cctgctcgtc
     2221 ccacaacagc ggcttcgtca gctccagata ccagtcgcag tattcgtgcc agacgaactc
     2281 gtacacggcg ttggcataga ggtcgaagcg gtaggtgttg atggcgcgtt cggacgtctc
     2341 caggaaagag cgcatctcgg agacgatcca gcggtcggcc aggctcagat cgcgcctgcc
     2401 cggctcttcg tcgtcggggc agttcatgag cacgaaacgg gccgcgttcc agagcttgtt
     2461 gcagaagttg cggtatccct cgatgcggct gaggtcgaaa cgcacgtcgc ggccggtgct
     2521 tgccagggcg cagtaggtga aacgcagggc gtccgttccg taggacggaa tgccttcagg
     2581 gaaatcggcc cgggtgttct tttcgatact cggggccatc tgcggttggg tgagattctg
     2641 ggttcgcttg tcgatcagct cgtccagaga gaccccgtcg atcaggtcta ggggatccag
     2701 gccgttgccc ttggtcttag acatcttctt tccatcggcg tcgcgcacca gcccgtgcac
     2761 gtagacctgc cggaacggca cctcgccggt gaacttcagc gtcatcatga tcatcctggc
     2821 cacccagaag aagatgatgt cgtgacccgt gacgagcacg tccgtggggt gaaactgaga
     2881 aagctcctcg gtctcctccg gccagcccag cgtcacgaac gtccacagcg cggaggagaa
     2941 ccaggtgtcc aggacgtccg ggtcctggtc caggggaacg tcgtcggcga ggccgccgtt
     3001 gctgcgggct tcgtcttcgt cccggcccac gtagacgttg ccttcggcgt cgtaccatgc
     3061 ggggatcctg tggccccacc actgctggcg cgagatggcc cagtcctgga tgttgcgcat
     3121 ccaggagaag tagacgttct cgtactgctt tggaacgaac tcgacgtcac cgttctcgac
     3181 agccttgatg gccgggtctg ccagcggctg gatcttcacg aaccactggt gagtcagcag
     3241 cggctcgacg atcgcgttcg agcggtctcc ccggggaacc atcagcgtat ggtcctcgat
     3301 cccgtccagg agcccctggc ttttcaggtc cgccaccact ttctcgcgag cgtcgaaccg
     3361 gtccagcccg cggtagggct ggggcgcggc ctcgtttatg gtggcgtcct cgttgaagac
     3421 gttgatcagc gcgagcccgt gtcgtgcgcc cacctcgttg tcgttgaaat cgtgggccgg
     3481 cgtgatcttc acgcagcccg agccgaactc cggatcgaca tagtcgtcgg cgacgatggg
     3541 gatctcgcga tcggcgagcg
//
LOCUS       DBNHOX010000661         1732 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_760149, whole genome shotgun sequence.
ACCESSION   DBNHOX010000661 DBNHOX010000000
VERSION     DBNHOX010000661.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_760149"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="AC16UZ_14485"
     CDS             <1..418
                     /locus_tag="AC16UZ_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6247943.1"
                     /translation="VIPSIADKIADIEFSSVFFTVFPNFHPWGSFNRINYRFRPNGRN
                     VDECIMECIYLAPIPENGEYTPCREIHWLGPDDDWTEAPELGMLAKVFNQDVRNLPYV
                     QEGLHATAKEHLQFADYNETKPRHLHMLMDEWIAKP"
     gene            complement(432..1580)
                     /locus_tag="AC16UZ_14490"
     CDS             complement(432..1580)
                     /locus_tag="AC16UZ_14490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6247944.1"
                     /translation="MSTDVAIIGIGIHPFGRTEGVSGLDQGVFAARQALKDVSLDWTD
                     MQFAYGGSAAAGSADTMVNKLGLTGLQFINVANGCATGGSALQSAFMAIQSGMFDIGM
                     AVGFDKHPRGAFTVDPEKRGLGKWYGDIGMALTPQFFAMKIQRYMHDYGITEDALTKV
                     AVKNYKNGALNPNAWRRNAFSYEEVAQSPMVCDPLRKYMFCSPAEGGVAMILCRADKA
                     AEYTSKPVFLDAVSVKTRNFGSFEVYTQGQALDRADSPTVMASKAAFEMAGIGPEDID
                     VAQLQDTESGAEIMHMAENGFCADGDQEKWLAEGRTEIDGRLPVNTDGGCLANGEPVG
                     ASGLRQVYEVALQLRGEAGERQVPNTPRTGYTHVYGAPGLSGVTILSR"
BASE COUNT          284 a          557 c          562 g          329 t
ORIGIN      
        1 ggtgattccc tcgatcgcgg acaagatcgc ggacatcgag ttctcgtccg tcttcttcac
       61 ggtctttccc aactttcacc cctgggggtc gttcaaccgg atcaactacc gtttccgtcc
      121 gaacggccgg aacgtggacg agtgcatcat ggagtgcatc tacctggcac cgattcccga
      181 gaacggcgag tacacgccgt gcagggagat tcactggctg gggcccgacg acgactggac
      241 ggaggcgccg gagctgggaa tgctggcgaa ggtgttcaac caggacgtgc gcaacctccc
      301 ctacgtgcag gagggtctgc acgccaccgc caaggagcac ctgcagttcg cggactacaa
      361 cgagaccaag ccgcgccacc tgcacatgct gatggacgag tggatcgcga agccctgacc
      421 cggcggtagc ttcagcgtga caggatggtg acgccgctca agcccggtgc gccgtacacg
      481 tgggtgtaac ccgtgcgggg tgtgttgggc acctgccgct caccggcctc tcctctgagc
      541 tgcagcgcca cctcgtagac ctgtcgcagg cctgacgcgc ccacgggctc gccgttggcc
      601 aggcagccgc cgtctgtgtt gaccggcagc ctgccgtcga tctccgtccg cccttcggcc
      661 agccacttct cctgatcgcc gtcggcgcag aagccgtttt ccgccatgtg catgatctcg
      721 gcgccggatt cggtgtcctg cagctgggcg acgtctatgt cctccgggcc gatgccggcc
      781 atctcgaagg cggccttgga cgccatcacc gtgggcgaat cggcccggtc gagcgcctgg
      841 ccctgggtgt aaacctcgaa ggagccgaag ttgcgagtct tcacgctgac cgcgtcgaga
      901 aagaccggct tgctggtgta ctcggccgcc ttgtccgcgc ggcacaggat catggccacg
      961 ccgccctcgg ccggtgagca gaacatgtac ttgcgcagcg ggtcgcagac catgggcgac
     1021 tgggccacct cctcgtagct gaaggcgttg cgtcgccagg cgttggggtt cagagcgccg
     1081 tttttgtagt tcttcaccgc caccttggtc agcgcgtcct cggtgatgcc gtagtcgtgc
     1141 atgtagcgct gaatcttcat ggcgaagaac tgcggcgtga gcgccatgcc gatgtccccg
     1201 taccacttgc ccagaccgcg cttttccgga tccacggtga aagcgccgcg cgggtgcttg
     1261 tcgaaaccca cggccatgcc gatgtcgaac atgcccgact gtatggccat gaaggcggat
     1321 tgcagagcgc tgccgccggt ggcgcagccg ttggcgacgt tgatgaactg cagcccggtg
     1381 agcccgagct tgttgaccat cgtgtctgcg gagccggcgg cggcgcttcc gccgtaggcg
     1441 aactgcatgt cggtccaatc gagactcacg tccttgagcg cctgccgcgc ggcgaacacg
     1501 ccctggtcca ggccggagac gccctccgtc cgtccaaaag ggtggatgcc gataccgatg
     1561 atcgctacgt ctgtagacat gcctgctcac ctcccggttt cagatgagca gattctacag
     1621 tattgtcttg ggctcggttg tcagggaggg atcgcatcat ggtcagagag acggggcagg
     1681 tgcccggtgt ggaggaggtg gccgatcggc tcgccatcct ggacgtgctc gc
//
LOCUS       DBNHOX010000662         3725 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_311003, whole genome shotgun sequence.
ACCESSION   DBNHOX010000662 DBNHOX010000000
VERSION     DBNHOX010000662.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_311003"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2438)
                     /locus_tag="AC16UZ_14495"
     CDS             complement(<1..2438)
                     /locus_tag="AC16UZ_14495"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6247945.1"
                     /translation="MNEGDRAGGSTARGAGVAFGLVLALLGSQASASAIEEIVVSARA
                     VEQSVRDVPVAITAFNEEVMDNMALTSFDDIAAYTPSVDILRISSGNGTSVSIRGISS
                     SAGSLGIEQSVAIVIDGVYYPQGRSINEGLFDTSQVAILKGPQALYFGKNATAGVISV
                     MTNDPGDELEILGKIGYETELKTTRAEGVLSFPINDRWGVRLALQGTDMSDGWLENTA
                     QDDVYVTFEGSQDNPAPKDAENPGEESFYGRVTLKGELSDAVTLRLKGSYSDFEYNTM
                     TLGEQYECSALNGVPHITLNGDPDPVTGWQRPTPLGLGECEANRARGLNPIPPAVAAT
                     SKDLSRFGGELGEDYESYSFTADLELDLENVFIKTFLNYHDQTVGWVIDADNNEKTNI
                     FASENNQFQNFSFETRAATRFDGPVNAVLGFYYQSTDRKFRQEVIFAGAEDSTADPTN
                     RFIAYDKISETDGETVSGYGELIWDVSEQWQVTAGARYIWENKDSFFTQPYVNFAFSG
                     LFVAGTLEDDSSFDDFIPEVTVRYQPNDDVTVYAAYKQGFKSGGFDNGAIYSTLNPDP
                     LADITFEPETVEGFEAGIKAALFDGTVNVELDAYYYEYEELQLNFFNSSVFAYRTLNA
                     GAAETTGSELQVKWAPPGVDGLTLLASVAYNDASYKDFIAPCSGGQSFSGGCNIGTPP
                     VPFGPPTLMDQNLKGEPRNLAPEWAGIVGFDFTRGIGSGLQIGLTGNMQWKSDYSLSE
                     FIPSARQDGFVWIDAALRFGAESGRWQLAFIGKNLTDEYVLVSARDTAQTGGNTGTDF
                     AYPADRIGTALRPRT"
     gene            complement(2584..>3725)
                     /locus_tag="AC16UZ_14500"
     CDS             complement(2584..>3725)
                     /locus_tag="AC16UZ_14500"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6247946.1"
                     /translation="WAGPMAGCMLADLGAEVIKVEHPDGEIGRVLGPHLPGGAHLSLL
                     YETANRNKRNLSVDMRKEVGRDLLIDLGRTCDLVVENFKPGTLHGWGLGYEHFCAAKP
                     DIVYVSISGFGQFGPHQARAAYDPLVQAQSGWMSLNGEIGGGPVKAPTFLGDDLAGMQ
                     AALAALAALRHRDATGEGQHVDVALMDAILGASNMFPSMARLGIEAPRTANQFPQVAP
                     FNVYACRGGSVFLGMSLNSHWEIFANAVGRPELVEDPRYATTELRTQNRESVDELVSR
                     WCSEQDQMRVVELLGGAGLPVAPVNDYRAATDDPNIEARDMLTDIELSDGSRAPLLGP
                     VPKFSRTPIGVRSGAPTIGQDNASILDELGIDDARRRALREAGVI"
BASE COUNT          631 a         1234 c         1186 g          674 t
ORIGIN      
        1 gttctgggcc gcagcgccgt gccgatccgg tccgcggggt aggcgaagtc cgtgcccgtg
       61 ttgccaccgg tctgtgccgt atcgcgagcg ctgaccagaa cgtactcgtc agtcaggttc
      121 ttgccgatga aggccagctg ccagcggccg ctctcagcgc cgaagcgcag cgccgcgtcg
      181 atccagacga agccgtcctg acgcgccgac gggatgaact cgctcaggga gtaatccgac
      241 ttccactgca tgttgccggt gaggccgatc tgcagcccgc tgccgatgcc ccgggtgaag
      301 tcgaagccca cgatgcccgc ccactcgggc gccaggttgc gcggctcgcc tttaaggttc
      361 tgatccatca gcgtcggagg accaaagggc acgggcgggg tgccgatgtt gcagccgccg
      421 ctgaaggact gcccgccgct gcacggggcg atgaagtcct tgtagctcgc atcgttgtag
      481 gccaccgagg ccaggagcgt caggccatcg acgcccggcg gcgcccattt cacctgcagc
      541 tcgctgcccg tggtctccgc cgcgccggcg ttcagcgtac ggtaagcgaa cacgctggag
      601 ttgaagaagt tcagctgcag ctcttcgtac tcgtagtagt aggcgtcgag ctccacgtta
      661 acagtaccgt cgaacagggc cgccttgatc ccggcttcga agccctccac cgtctccggc
      721 tcgaaggtaa tgtcggccag cgggtccggg ttcagagtgc tgtagatggc gccgttgtcg
      781 aagccgccgg acttgaagcc ctgcttgtag gccgcgtaaa cggtgacgtc gtcgttgggc
      841 tgataacgga cggtcacctc agggatgaag tcgtcaaagg agctgtcgtc ttccaaagtg
      901 cccgccacga agagccctga gaaagcgaag ttcacgtagg gctgggtgaa aaacgagtcc
      961 ttgttctccc agatgtagcg cgcgcctgcc gtgacctgcc actgctcgga aacgtcccag
     1021 atcagctccc cgtagccgga gaccgtctcg ccgtcggtct cgctgatctt gtcgtaggcg
     1081 atgaagcgat tggtcggatc cgccgtggag tcctccgcgc cggcgaagat cacctcctgg
     1141 cggaacttcc ggtccgtgct ctggtagtag aagcccagca cggcgttgac ggggccgtca
     1201 aaccgggtgg ccgcgcgggt ctcgaaggag aagttctgga actggttgtt ctcgctggcg
     1261 aagatgttgg tcttctcatt gttgtccgcg tcgatgaccc agcccacggt ctggtcgtga
     1321 tagttgagga acgtcttgat gaacacattc tccagatcga gctccagatc agccgtgaag
     1381 ctgtaggact cgtagtcctc gcccagctcc ccgccgaagc ggctgaggtc cttggacgtc
     1441 gccgcgactg ccggcggtat gggattcagc ccacgcgccc gattcgcctc gcactctccc
     1501 aggcccagcg gcgtcggccg ctgccagccg gtgaccgggt cagggtcgcc attcagggtg
     1561 atgtggggaa cgccattgag cgcagagcac tcgtactgtt cccccagggt catggtgttg
     1621 tactcgaaat ccgagtacga ccccttgagc ctcagggtca ccgcgtcgct gagctcgccc
     1681 ttgagggtca cgcggccgta gaacgactct tccccggggt tctccgcgtc tttgggcgca
     1741 ggattatcct gactgccctc gaacgtaacg taaacgtcgt cctgcgccgt gttctcgagc
     1801 cagccgtcgg acatgtcggt gccctgcagc gccagacgca caccccatcg gtcgttgatg
     1861 gggaaagaca ggaccccctc ggcgcgggtg gtcttgagct ccgtctcgta gccgatcttg
     1921 cccaggatct ccagctcgtc gccgggatcg ttggtcatca ccgagatcac cccggcagtg
     1981 gcgttcttgc cgaaatacag cgcctggggc cctttgagaa tcgccacctg gctggtgtcg
     2041 aagaggcctt cgttgatgga gcggccctgg gggtagtaga ccccgtcgat gacgatcgca
     2101 acggactgct cgatgcccag gctcccggca ctggaggata tcccccggat gctgacggac
     2161 gttccgttgc cactcgatat gcgaaggatg tccaccgatg gggtatatgc cgcgatgtcg
     2221 tcgaagctgg tcagcgccat gttgtccatg acctcctcgt tgaacgccgt aatggccacc
     2281 ggcacgtccc gaacggactg ctccacggcc cgggcggaaa ccacgatctc ctcgatggcg
     2341 ctggcagacg cctggctacc gagcagcgcc aggaccaggc cgaaagcgac gccggcccca
     2401 cgtgcggtcg acccgccagc tctatcgccc tcgttcatgc gcgcacccat gcacacagag
     2461 aatctcgccg ttctcatatc taccctcccc agggatggat tcaactcttt tgattatgtg
     2521 cgccttccgg acctgcaggc aaccaccccg acgcttcggc tcacgcagca gcgacggccc
     2581 cgttcatatc accccggcct cccgcaacgc ccggcgccgc gcgtcgtcga tgccgagctc
     2641 atcgagaatt gaggcgttgt cctgcccgat ggtcggcgcg cccgagcgca cgccgattgg
     2701 cgtacgggag aacttcggca ccggcccgag caacggagcc cggctgccat ccgagagctc
     2761 gatgtcggtc agcatgtcgc gggcttcgat gttgggatcg tccgtcgcgg cgcggtagtc
     2821 gttgaccggc gccacgggca ggcccgcgcc gccgagcagc tccaccacgc gcatctggtc
     2881 ctgctcgctg caccaccgcg aaacaagctc atcgacgctc tcccgattct gcgtgcgcag
     2941 ctcggtggtc gcgtaacggg gatcctcgac cagctccggg cgccccacgg cattggcaaa
     3001 gatctcccag tgggagttca gcgacatgcc gagaaagacc gagccgccgc ggcaggcgta
     3061 gacgttgaag ggcgctacct ggggaaactg gttcgccgtg cggggcgctt cgatgccgag
     3121 ccgcgccatg gacgggaaca tgttggaggc gccgagaatg gcgtccatca gcgcaacgtc
     3181 cacgtgctgg ccctcaccgg tggcgtcccg atgtcgaagc gcagccagcg cggccagcgc
     3241 tgcctgcatc ccggccaggt catcgcccag gaaggtgggc gccttgaccg ggccgccgcc
     3301 gatctctccg ttcagggaca tccaccccga ttgcgcctgc accagcgggt cgtaggcggc
     3361 ccgtgcctgg tgtggcccga actgaccgaa cccgctgatg gaaacgtaga cgatgtcggg
     3421 ctttgccgcg cagaagtgct cgtaacccag gccccaaccg tgcagggttc cgggcttgaa
     3481 gttctccacc accagatcgc acgtccggcc gaggtcgatg agcaggtcgc gaccaacctc
     3541 cttgcgcatg tcaacgctca ggttgcgttt gttgcggttg gcggtctcgt aaagaagaga
     3601 aaggtgagcg cctcccggca gatgcgggcc cagaacccgg ccgatctcgc cgtccgggtg
     3661 ctccaccttg atgacctcgg cacccagatc ggcgagcatg cagccggcca tcggcccggc
     3721 ccagg
//
LOCUS       DBNHOX010000663         1891 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1595774, whole genome shotgun sequence.
ACCESSION   DBNHOX010000663 DBNHOX010000000
VERSION     DBNHOX010000663.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1595774"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="AC16UZ_14505"
     CDS             complement(<1..471)
                     /locus_tag="AC16UZ_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015371271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR6247947.1"
                     /translation="MIYDNILQTIGNTPIVRLNRTGPDHVEMYVKVEAFNPLASVKDR
                     LAYAIIKDATDSGALQPGQTVVEATSGNTGIALAMVCAATGHPFVATMVETFSIERRK
                     IMRALGAKVILTPAAERGSGMVRRAEELAAEHGWFLARQFENPANPAYHRNTTGP"
     gene            complement(588..1142)
                     /locus_tag="AC16UZ_14510"
     CDS             complement(588..1142)
                     /locus_tag="AC16UZ_14510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6247948.1"
                     /translation="MTQPARETLAAMLQVHLGRLRRLALVLTENPDDAEDLVQDVVVK
                     LCGRPHEVAGVRDLSTWLNRVVYNQFIDDRRRRKRQPLQLVGSADELDAAMPGESAEP
                     MLEALCKDRDQRLGDAVNALNAEQRMVLLLHDSQGYRLTEIHQLTDIPLGTLKSRLHR
                     ARSRMRVLLDSERVPDDRSEPDQG"
     gene            complement(1307..1654)
                     /locus_tag="AC16UZ_14515"
     CDS             complement(1307..1654)
                     /locus_tag="AC16UZ_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247949.1"
                     /translation="MSTTNPGNDPHKDPLDALLSEAFQQDIEQVGDADMAGRVMRTLK
                     RRTRLRAAILGVSMAAGLSLATALALPGLTDLLRLLAEQAPVEPSSWGVLPIAALLAI
                     LTPWLFSLVDDRV"
     gene            complement(1651..>1891)
                     /locus_tag="AC16UZ_14520"
     CDS             complement(1651..>1891)
                     /locus_tag="AC16UZ_14520"
                     /inference="COORDINATES: protein motif:HMM:NF019886.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6247950.1"
                     /translation="AGQTALEGPAPNADELPDLPRLLAILPEADRLVLLLSYGHGLSI
                     AEIADVLETPDGTIKSQIHRAKDKIRRHFSIGAAA"
BASE COUNT          300 a          581 c          652 g          358 t
ORIGIN      
        1 ggggccggtg gtgtttctgt ggtaggccgg attggccggg ttctcgaact gtcgcgccag
       61 gaaccagccg tgctcggcag ccagctcctc cgctcgacgg accataccgc tgccgcgctc
      121 tgcggcgggt gtgagaatga ccttcgcgcc cagggcgcgc atgatcttgc gccgctcgat
      181 ggagaacgtc tccaccatgg tggcgacgaa cggatgtccc gtggccgcgc agaccatggc
      241 gagcgcgatg cccgtgttgc ccgacgtggc ctcgaccacg gtctgtcccg gctgcagcgc
      301 gccggagtcc gtggcgtcct tgatgatggc gtaggcgagg cggtccttca cggatgccag
      361 cgggttgaac gcctcgacct tcacgtacat ctccacgtgg tcggggccgg tccgattgag
      421 cctgacgatc ggcgtgttgc cgatggtctg caggatgttg tcgtaaatca tcgggatgcc
      481 actccgtgtg cgaacttcgc tgcagcccac gataccccaa tggggtttgc ctcatccagg
      541 tgggcgtagc cggtcggggc gtttcgagtt cttccggggc ggttcggtca gccctggtca
      601 ggttctgatc ggtcgtccgg gaccctctcg ctgtccagca gcacgcgcat tcgggagcgg
      661 gcccgatgca gcctggattt cagagtgccc agcggaatgt ccgtaagctg gtgaatctcg
      721 gtgagacggt agccctgcga atcgtgcagg agcagcacca tcctctgctc ggcgttcaga
      781 gcgttcacgg cgtcccccag gcgttggtcg cggtctttgc agagggcctc cagcatcggc
      841 tcggcggact cgccgggcat ggcggcatcg agctcgtcgg ccgacccgac cagctgcagc
      901 ggctggcgct ttcgccggcg ccggtcgtcg atgaactggt tatagacaac gcggttgagc
      961 catgtggaaa gatcccggac gccagcgact tcgtgcggcc gtccgcagag cttgactacc
     1021 acgtcctgca ccaggtcctc ggcgtcgtcc gggttttccg tgagaaccag ggccaggcgt
     1081 cggagcctgc ccagatgaac ctgcagcatg gctgcaaggg tttcccgagc gggttgagtc
     1141 atgggaggtt tcgtggtcct gatcaaactg cgatcgcgga tcagtgttgc acctcggacc
     1201 ggcaaaggct cgaacgtgtg cccacaatgg gtgcctggcg gaagggaaaa gtgtgcgcga
     1261 gcccgcaaag ttgagcgagc gcgccgtcgc gatgcgctcc ttcaggtcag acgcggtcgt
     1321 ccaccaggga gaagagccag ggcgtgagga tggccagaag cgcagcgatg ggcaggactc
     1381 cccaggaaga gggctcaacg ggggcctgct ctgcgagcag gcgcagcagg tcggtaaggc
     1441 ccggcagggc cagggctgtt gccaacgaaa gacccgctgc catgctcacc cccaggatgg
     1501 cggcgcgcag cctggtgcgc cgcttgagag ttcgcataac ccggccagcc atgtctgcat
     1561 cgccgacctg ctcgatgtcc tgttggaacg cctcgctgag cagggcgtcg agaggatcct
     1621 tgtgaggatc gttgccagga ttggtagtgc tcatgctgcg gcccctatgc tgaagtgcct
     1681 gcgaatcttg tcttttgccc ggtggatctg ggattttatc gtaccgtccg gcgtttcgag
     1741 aacgtcggcg atttctgcga tgctcagccc gtggccgtaa ctcagcagca gcacgagccg
     1801 gtcggcctcg ggaagtatcg ccagcagacg aggcaggtcc ggcaactcgt cggcatttgg
     1861 cgccgggccc tcgagcgccg tctgccctgc c
//
LOCUS       DBNHOX010000664         1770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_567800, whole genome shotgun sequence.
ACCESSION   DBNHOX010000664 DBNHOX010000000
VERSION     DBNHOX010000664.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_567800"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="AC16UZ_14525"
     CDS             <1..1074
                     /locus_tag="AC16UZ_14525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR6247951.1"
                     /translation="NAYFQVNQRRGVRWSASKAFLRPAMARPNLEVLTNAHVQHLEIE
                     TTDAGLRASGVTVQMGDDAPTTITARREVVLAAGAIGSPQILQLSGVGPGDLLHEHGI
                     RVIHDLPGVGENLHDHLQVRSVYKVHGTVTLNQRANSLIGKALMGLEYFTRKTGPLTM
                     PPSQLGAFAKSDPSVASADIEWHVQPLSLDAFGEPLHRFNAITPSVCNLRPTSRGHLR
                     IKSPDPADYPAITLNYLATEHDRQVILAGMRLTRRIMAAKALERFQPEEWKPGPSLTT
                     DEELLTAAGDLGTTIFHPVGSCRMGSDPMAVVGDRLAVHGVEGLRVIDASVMPQITSG
                     NTNAPTVMIAEKGAEFMRADAAS"
     gene            complement(1084..1590)
                     /locus_tag="AC16UZ_14530"
     CDS             complement(1084..1590)
                     /locus_tag="AC16UZ_14530"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR6247952.1"
                     /translation="MSESLDKALTVARDRVVRFHYVIRDEAGSELESTREGEPLAVLQ
                     GRGSVLRGVEEALEGRNAGERLEVTLPPEQAFGARREDWTQRVPKKYLPDAKRLKPGD
                     AAVLRTREGPRPVTVVKVGSKVVDVDLNHPRAGMTLTFDLEIVEVREATPEEIAHRHV
                     HGPGGHRH"
BASE COUNT          304 a          642 c          545 g          279 t
ORIGIN      
        1 aatgcctact ttcaggtgaa ccagcggcgc ggggtgcgct ggagcgcaag caaagccttc
       61 ctgcgcccgg ccatggcgcg tcccaatctc gaggtgctca cgaacgccca cgttcagcac
      121 ctggaaatcg agaccaccga cgccggcctt cgcgcctccg gcgtcaccgt gcagatgggc
      181 gacgacgcgc ccaccaccat caccgcgcgc cgggaagtgg tgcttgccgc aggcgccatc
      241 ggctcccccc agattctgca gctctccggt gttggccccg gcgatctgct gcacgagcac
      301 ggcatccggg tgatccacga cctgccgggc gtgggggaga acctccacga ccacctgcag
      361 gtgcgctccg tctacaaggt gcacggcacg gtgacgttga atcagcgggc gaactccctc
      421 atcggcaagg cgctgatggg gctggagtac ttcaccagga aaaccggacc actcaccatg
      481 ccgccctctc agctgggcgc gttcgcgaag tccgatccgt cggtcgcctc cgcggacatc
      541 gagtggcacg ttcagccgct gtcgctggac gccttcgggg agccgctgca ccgcttcaac
      601 gcgatcaccc cgtcggtgtg caacttgcgg cccacgagtc gcggacatct gcgaatcaag
      661 agcccggacc ccgccgatta tccggcgatc accctcaact acctcgcaac cgagcacgac
      721 cgtcaggtca tcctcgccgg gatgcggctc acccgccgga tcatggcggc aaaggcactg
      781 gagcggtttc aaccggagga gtggaagccc ggccccagcc tgactaccga cgaggagctg
      841 ctcaccgccg ccggcgatct cggcaccacc atctttcacc cggtgggcag ctgcagaatg
      901 ggcagcgacc ccatggctgt ggtcggcgac cggctggccg tgcacggcgt tgaagggctg
      961 cgggtgatcg acgcttcggt aatgccgcag atcacctccg gcaacaccaa cgcgcccacg
     1021 gtgatgatcg ccgagaaggg cgcggagttc atgcgggccg acgcggcctc ctgagacacc
     1081 gcctcagtgc cgatggcccc cggggccgtg cacgtggcgg tgggcgatct cctcgggcgt
     1141 ggcttcgcgc acctccacga tctcgagatc gaacgtcagg gtcatccccg cgcggggatg
     1201 gttgagatcc acgtccacga ctttgctccc caccttcacc acggtgaccg gacgcggccc
     1261 ctcccgggtc cgcagcacgg ccgcatcgcc aggtttgagc cgtttggcat cgggcagata
     1321 tttcttggga acgcgctggg tccagtcttc ccgccgggcg ccgaaagcct gctcgggagg
     1381 aagggttact tccaggcgct cgccggcgtt tcggccctcc agcgcttcct cgacgccccg
     1441 cagcacgctg ccgcgcccct gcagcacggc cagaggctcg ccttcgcgcg tggactcgag
     1501 ctccgagccc gcctcgtcgc gaatcacgta gtgaaagcgc accaccctat cccgcgccac
     1561 ggtgagggcc ttgtccaaag actccgacat cactttctcc ccgatagact tctcatccca
     1621 cctgccaccc gggcaccacg aacaccagcc acgccaggat gggcatcagc gcgaccatga
     1681 cgatggtgta cttgagctgg gcctgcaaga tctcctggcg gttcagcacc tgaccagccg
     1741 tagccaggaa catcgcgccc caggtggaat
//
LOCUS       DBNHOX010000665         3416 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_183374, whole genome shotgun sequence.
ACCESSION   DBNHOX010000665 DBNHOX010000000
VERSION     DBNHOX010000665.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_183374"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1841
                     /gene="pgaA"
                     /locus_tag="AC16UZ_14535"
     CDS             <1..1841
                     /gene="pgaA"
                     /locus_tag="AC16UZ_14535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HIR6247953.1"
                     /translation="GEARRRWIMLASALGATVEAFEAAQANPEVLTPDDVVRLQLDAA
                     AKRIRWADLPSPGAPLADAEMAVDGHDVVAKGGEPARVAGSHRRALEYDRVVSHSAAY
                     RMVEAIDLFESLQGTPGDLEGFPSYVLAAAGRAYLYEEHTRDAIACFEQALGLDPNAF
                     NLRIGLFYALSDHGDFEGAKEVAEHLIAGEEPFARPDPRVWVPNDRYATARVIAAREL
                     AYRERYTEALNDLDEMLAIAPANTGVRLTRAQLLQWRGWNEQAEREVVRIQTTDPTNV
                     GVKVLSGTVALEKQQYPRAEREVNRALRSQPYDKSAIELNERWLLHNSGYFEASASAE
                     RSEGSAFAGESWRTDVHYYSSPIAYRYRWFVHDAIRHGKFTEGTGKDHRIGAGVDFRS
                     NRWSARGEVHKGLEQNRKAGVTASASWRQSDRLTWDSLVSYNTMGMPLRGTRVGIRAD
                     EAQGGATYRWHESRRAYARAGLMKMDDGNDRKWLDSGFEQRVVNAPRQKLLVNLGAYT
                     SRNSGGDRVYYNPETDYQITAGLTHEWRLMRRYQQSFVQRLSVDGGEYHQKGFGGGPI
                     WTLRLEHDWQIGRRVGMSWGATWGGRRYDGEREHVTTVFLTFRGHL"
     gene            1838..>3416
                     /gene="pgaB"
                     /locus_tag="AC16UZ_14540"
     CDS             1838..>3416
                     /gene="pgaB"
                     /locus_tag="AC16UZ_14540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HIR6247954.1"
                     /translation="MRRRLAVIAAALALIVGAHGAGAESAHRIIAISYHDVVEHPSEV
                     GADRFTVTVDQLINQLAWLDAHGFEPITLDQWAAAAEGEPLPPRPVLLTFDDGYASFA
                     EHVMPVLKLFDFPAVLAPVTSWVDAPEGSVVQYGDQPMPRERFLTWDQLREISKTGLV
                     EIATHTHDMHRGLTGNQFGNELPAAVTRRWNDERNAYEPEADYEARIRSDLKRSRDRI
                     AEQLGKPPRAVVWPYGAYNLQAASVARTLGLTYTLTLDSLPNDPRIDGGRIHRELVST
                     DTSMLFFTTWALGKEQETPLRAAHVDMDYVYDPDPAQAERNLGRLLDRIKAMKLSFVF
                     LQAFADPDGDGVADALYFRNRHLPMRADLFNRVAWQLKTRAGVEVFAWMPVMAFELPD
                     AERNQALAVKAYDGSHESRYHRLSPYNPEARRIVGEIYDDLGRGSRFLGVLYHDDGFL
                     TDREDVSPAALARTVPDEAARGDGVLAPAVVSDFRGKTRFLIDFTHELSDVLRRHQPA
                     LLTARNLYARPVVDPQAE"
BASE COUNT          608 a         1117 c         1138 g          553 t
ORIGIN      
        1 tgggggaagc gcgccgacgc tggatcatgc tggcatccgc tctcggggca accgtcgagg
       61 cgtttgaagc cgctcaggcc aatcccgagg tgctcacgcc cgacgacgtg gtgcgcctgc
      121 agctcgatgc ggcggcgaaa cgaatccgct gggccgatct gccgagcccg ggtgccccgc
      181 tggcggacgc cgaaatggcc gtggacggcc atgatgtggt tgccaaaggc ggagaaccgg
      241 cacgggtcgc aggtagccac cgacgcgcgt tggagtacga ccgcgtcgtc tcccattccg
      301 cagcgtaccg gatggtggag gccatcgacc tcttcgagtc gttgcagggt acgcccggcg
      361 atctggaagg atttccttcc tacgttctcg ccgccgccgg ccgcgcttac ctgtacgaag
      421 agcatacccg cgatgccatt gcctgcttcg agcaggcgct cgggctcgac ccgaacgctt
      481 tcaaccttcg catcgggctt ttctacgcgc tgtccgatca cggtgacttc gaaggcgcga
      541 aagaggttgc cgagcacctg attgccggcg aagaaccgtt cgcccgaccg gatccgcgag
      601 tctgggtgcc caacgaccgc tacgccacgg cgcgggtcat tgcggctcgg gagctcgcct
      661 atcgggaacg ctacaccgag gcgctcaacg atctggacga gatgctcgcc atcgcgccgg
      721 ccaacacggg cgtgcggctc acgcgagcgc aattgctgca gtggcgtggc tggaacgagc
      781 aggccgagcg cgaagtggtg cggatccaga cgaccgaccc gaccaacgtt ggcgtcaaag
      841 tgctgagcgg cacggttgcc ctcgaaaaac agcagtaccc gcgagccgag cgggaagtta
      901 atcgcgctct tcgatcacag ccctatgaca agtccgccat cgagctcaac gagcgatggc
      961 ttctgcacaa cagcgggtat ttcgaggcca gcgccagcgc ggagcgatcc gaggggtcgg
     1021 cctttgccgg cgagagctgg cgcacggacg tgcactacta ctcgagcccc atcgcctacc
     1081 gctaccggtg gttcgttcac gacgccatcc gccacggcaa gttcacggaa ggaaccggca
     1141 aggatcaccg catcggcgcg ggcgtggatt tccgcagcaa tcgctggagc gcgcgcggcg
     1201 aggtgcacaa gggcctggag cagaaccgta aggcgggcgt gacggcatcg gcgagctggc
     1261 ggcagtccga tcgtctcacc tgggacagcc tggtctccta caacaccatg gggatgccgt
     1321 tgcgcggcac ccgcgtcggc atccgcgcgg acgaggctca gggtggcgcg acctatcgct
     1381 ggcacgaatc ccgacgcgcg tacgcgcgtg cgggcctgat gaagatggac gacggcaacg
     1441 accgcaagtg gctggattcc ggcttcgagc agcgcgtggt caacgcccct cggcagaagc
     1501 ttctcgtcaa cctgggagcg tacacgagcc ggaactccgg cggcgaccgg gtctactaca
     1561 acccggagac cgactaccag atcacggcgg gcctgaccca cgagtggcgt ctgatgcggc
     1621 gctaccagca gtccttcgtt cagcggctga gtgtggacgg cggtgagtac caccagaagg
     1681 gtttcggtgg cggcccgata tggaccttgc ggctcgaaca cgactggcag atcgggcgcc
     1741 gggtgggcat gagctgggga gccacctggg gcgggcgccg ctacgacggc gagcgcgagc
     1801 acgtgaccac cgtgttcctg actttccggg gacatctgtg aggcggcggc tggcagtcat
     1861 cgccgccgcg ctggcgctca tcgtcggcgc gcatggtgcc ggtgccgaat cggcccatcg
     1921 gatcatcgcg atctcctacc acgacgtggt ggagcatccc tctgaggtcg gggccgaccg
     1981 cttcacagtg accgtagatc agctcatcaa tcagctggcc tggctcgacg cccacggctt
     2041 cgagcccatc accctcgacc agtgggcggc tgcagccgag ggagagccgc tcccgccccg
     2101 gccggtgctt ctcaccttcg acgacggcta cgcgagcttt gcagagcacg tgatgcccgt
     2161 tctgaaactt ttcgattttc cggccgtgct cgcgccggtg accagctggg tggacgcccc
     2221 cgaaggtagc gtcgtccagt acggcgacca gcccatgccg cgggagcgat ttctgacgtg
     2281 ggatcagctc agagagatat cgaagaccgg cctcgtggag atcgcaacgc atacccacga
     2341 catgcatcgt gggctcacgg gcaaccagtt cggcaacgag ttgcctgctg ctgtgactcg
     2401 aaggtggaac gacgagagga acgcctacga gccggaggcc gattacgagg cgcgcatcag
     2461 aagtgacctc aagcgcagcc gggaccgcat cgccgagcaa ctcggcaagc cgcctcgggc
     2521 cgtggtgtgg ccctacggag cctacaacct tcaggcggca agcgtcgccc ggaccctcgg
     2581 gctcacgtac acgctgacgc tggacagcct gccgaacgat ccgcgcatcg atggcggccg
     2641 cattcatcgc gagctggtga gcaccgacac gtcaatgctg tttttcacca cgtgggccct
     2701 gggaaaggag caggagacac cgctgcgggc agcgcacgtg gacatggatt acgtgtacga
     2761 cccggacccg gcccaggccg aacgaaatct cggccggctc ctggatcgta tcaaggcgat
     2821 gaagttgtcc tttgtcttcc tgcaggcctt cgcagatccc gacggcgacg gcgtcgcgga
     2881 cgcgctgtac ttccgcaaca ggcacctgcc catgcgtgcc gatcttttca accgggtggc
     2941 ctggcagctg aaaaccaggg cgggcgtgga ggtgtttgcc tggatgccgg tcatggcgtt
     3001 cgagctgcct gatgccgaac gcaatcaagc gcttgcggtg aaggcctatg acgggtccca
     3061 cgagtcccgc taccaccggc tgtctccgta caacccggag gcccggcgca tcgtggggga
     3121 gatctatgac gatctcggtc gcggcagccg ctttctgggc gtgctctacc acgatgatgg
     3181 gttcctgacc gatcgcgagg acgtcagccc ggccgctctc gcccgcaccg tccccgacga
     3241 ggcagcgaga ggcgacggcg ttctggcgcc ggcggtcgtg agcgacttcc gcggcaaaac
     3301 ccgattcctc atcgatttca ctcacgagct ttcggacgtg ctgcgtcggc accagcccgc
     3361 gctgctcacc gcgcgaaacc tgtacgcgag gccggtcgtg gatccccagg ccgagg
//
LOCUS       DBNHOX010000666         5259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1147425, whole genome shotgun sequence.
ACCESSION   DBNHOX010000666 DBNHOX010000000
VERSION     DBNHOX010000666.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1147425"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..799
                     /locus_tag="AC16UZ_14545"
     CDS             230..799
                     /locus_tag="AC16UZ_14545"
                     /inference="COORDINATES: protein motif:HMM:NF014534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HIR6247955.1"
                     /translation="MQIPLQITWRNIPKSEALEADIVAKAEKLEEFFDHILSCRVVVE
                     KSHGRHRKGNLYRLRLDIHVPDKEIVVTRDPGDDHAHEDMYVSIRDAFDAARRQLQDY
                     VRVRRGHVKPHDQHHVGRVLRRIAAEDYGFLGTADGREIYFHRNSVVNADFDKLEPGT
                     EVMFVEETMNEGVQAKMVSVGKHRFPEGS"
     gene            995..2146
                     /locus_tag="AC16UZ_14550"
     CDS             995..2146
                     /locus_tag="AC16UZ_14550"
                     /inference="COORDINATES: protein motif:HMM:NF018172.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - aliphatic amine dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amine dehydrogenase large subunit"
                     /protein_id="HIR6247956.1"
                     /translation="MRVFVVFSVLLAASGMSLAADQFEAEPLGEVAQLPSPNPDHWVV
                     IHDLSFNHMREGKLFLVDPHADSVSGQLRGMMSADFVASFLVSARRNEYYVIESFHAR
                     GGRGGERTDVVTIYDPATLSVLEEIIIPPKRLSGMPNRYQVALLDDDRYMVVFNFTPS
                     QSATVVDLERRQVLGEVAVPGCMFAYPTGKRGFSSLCSDGGMLTTRLGEDGSVAGTDR
                     LAPFNDVIDDAMFARPAEIDGVFYFPTFTGNVAPIDMRGEAAALGERWSLTTASERAQ
                     GWRPGGLWPAVGDGNGRLYMLMHGHGAEGTHKDGGDQVWIFDAANGKRIGKIELARWG
                     IALSVSTGERPQLMVSGAEPVIDVYEADGTHVRTLGVDMSTPLLVHGVR"
     gene            2166..2687
                     /locus_tag="AC16UZ_14555"
     CDS             2166..2687
                     /locus_tag="AC16UZ_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase light chain"
                     /protein_id="HIR6247957.1"
                     /translation="MNWLDKLSEKSTRGLAQQTSRRGWLAGIGTVLAGAASVPLLPMA
                     RASSGGQPKLEDVADTGDPLSCDYWRHCSIDGFLCGCCGGSHNTCPPGTEMSPITWVG
                     TCRNPVDGKNYVISYNDCCGKTPCGQCFCNTNQGDRPMYRPDKNNDINWCLGTESTVY
                     HCSTSVLIGLAVE"
     gene            2684..3112
                     /locus_tag="AC16UZ_14560"
     CDS             2684..3112
                     /locus_tag="AC16UZ_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6247958.1"
                     /translation="MKGRTVCFAFLIAAAPHAIGDGPVDAERARLNYMLNCQGCHLPD
                     GSGVEDAVPRMNGFVGNFLHVPGGREFLVRVPGSANAALDDASLAELLNWILVTMSPG
                     ELPPSWVPYSAGEVAELRSAPLREVEKVRAGLVRSIQQHL"
     gene            complement(3173..3811)
                     /locus_tag="AC16UZ_14565"
     CDS             complement(3173..3811)
                     /locus_tag="AC16UZ_14565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HIR6247959.1"
                     /translation="MLKLYALFGDDGAESPFQLNQAQALKVLQERFPELRRYRQFLRT
                     GEQLGNLVLAPFRASAEFWLAEHDDPTPLMAALETAAEDVHEVFAVNPRVLIRSEEHN
                     ISGEPFHDSGFKATFLFDRKPSLSLEEFHDYWLTHHGPIAAQTQDAERYVQSHIRTAQ
                     RAYDGVTELFWPDYPTALASMASEQMTTDQANDAPNFVDGESLVVFLAREAN"
     gene            complement(3815..4342)
                     /locus_tag="AC16UZ_14570"
     CDS             complement(3815..4342)
                     /locus_tag="AC16UZ_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase light chain"
                     /protein_id="HIR6247960.1"
                     /translation="MNWLDRITQKTTRKVARATSRRGFLAGASAVLLGASATPLLPVA
                     RASESRVPPQSTGNPEDPGDPTQCDYWRYCAIDGFLCSCCGGTQNACPPGTEMSPLTW
                     IGTCRNPVDGKNYVISYNDCCGKSSCGRCLCNRNEDDRPVYRPSANNDINWCMGTTSS
                     VYNCSTAVVLGLATE"
     gene            complement(4354..>5259)
                     /locus_tag="AC16UZ_14575"
     CDS             complement(4354..>5259)
                     /locus_tag="AC16UZ_14575"
                     /inference="COORDINATES: protein motif:HMM:NF018172.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - aliphatic amine dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amine dehydrogenase large subunit"
                     /protein_id="HIR6247961.1"
                     /translation="NLSNSRQEFYVGETLHSRGSRGQRQDVIAIYDFANLDVVAEVDL
                     PPRRANTVINLGNSRLTEDERFLLVFMQNPGTSVAVIDLDSRKMVSEISTPGCALIYP
                     TVERSFFMLCGDGRMLSITLDETGNPVSRKASEPFIDIDADPLSEKSAKAGGIWHFVS
                     YRGQVQPIDARAAQPELMTTWWLATEKERAANWRPAGWHWTAGHKSGLLWVGMTPDGY
                     DGSHKDPAPEIWLFDTGERERLARIELRAPAISISVTQHEKPYLLVASTEATLDVYDA
                     MSGEYLRSIYDLGFTPYMVHPVSYE"
BASE COUNT          900 a         1612 c         1762 g          985 t
ORIGIN      
        1 gaggtccggc tcacgcgcga ggacggaacc ctgctggcca tctaccgggg gacctgctcc
       61 aatgcttcct cggattctcg ctaaccccgg cagccggggt tcgcggggcg tgccgggttc
      121 ggctgcacgc ctaccggccc ccgcggggaa ctacgtggaa atccggagcg ccaatcggaa
      181 cccaaaggcg catctttgga cgtgtgtaga acgtgatggg agttgcgact tgcagatacc
      241 cttgcagatt acctggcgaa acatccccaa aagcgaagcc ctggaggccg acatcgtcgc
      301 gaaggcggag aagctcgaag agtttttcga ccacatcttg agctgccgtg tggtggtgga
      361 gaagtctcat gggcggcacc ggaagggtaa tctctaccgc ctgcggctcg acatccacgt
      421 cccggacaaa gagatcgtgg tgacgcgtga tccgggggac gatcacgccc atgaagacat
      481 gtacgtgagc atccgcgatg ccttcgacgc cgctcggcgg cagctccagg actacgtccg
      541 cgtcaggcgc ggacacgtca agcctcacga tcagcatcat gttggccggg ttctgcgaag
      601 gatcgccgcg gaggactacg gctttctcgg aacggccgac ggtcgcgaga tctactttca
      661 tcgtaactcg gtggtcaatg cggacttcga caagctcgag cccggtaccg aggtgatgtt
      721 cgtggaggaa accatgaacg aaggcgttca ggcgaagatg gtttccgtgg gcaagcaccg
      781 atttcccgaa ggttcctgat ctcggtggcg gaatgccgga cggctcagcc gccgccatcc
      841 ccgccggcag agtgccgtcc cgccgcgtga tcggcaatgg gtggggtcgt gaatttctgt
      901 ctccgccctg tcgattggaa gcaggtagtg ccgattggtt ggtgatcgcc aacgcgcggg
      961 ttacacttcg gccagggatc agtgggagga atccatgcgg gttttcgtcg tcttcagcgt
     1021 gctgctcgct gcatccggca tgagccttgc ggccgaccag ttcgaggccg agcctctggg
     1081 tgaggttgcc cagctgccgt cccccaatcc ggatcactgg gtggtgattc acgatctgtc
     1141 cttcaaccac atgcgggagg gcaagctctt tctcgtcgat ccccatgcgg actcggtgtc
     1201 cggccagctg cgcggcatga tgtccgccga tttcgtcgcc agcttcctcg tcagcgcccg
     1261 gcgcaacgag tattacgtga tcgagagctt ccacgcccgc ggaggtcggg gcggcgaacg
     1321 caccgacgtc gtcaccatct acgatccagc caccctgtcg gtgctggaag aaatcatcat
     1381 tccgccgaag cgcctgtcag gcatgcccaa ccgttatcag gtggccctgc tcgacgacga
     1441 tcggtacatg gtcgtcttca acttcacgcc gtcgcagtct gccaccgtgg tggatctcga
     1501 gcgtcggcag gtgctagggg aggtagctgt tcccggatgc atgtttgcct acccgaccgg
     1561 caaacgcggg ttcagctcgc tgtgcagcga cggggggatg ctgacgaccc ggcttggcga
     1621 agacggatcg gtcgcgggaa cggatcggct ggcgccattc aacgacgtca tcgacgatgc
     1681 catgttcgcc cggccggcag agatcgacgg cgtgttttac tttccgacgt ttacgggcaa
     1741 cgtggctccc atcgatatgc gcggcgaagc ggccgcgctg ggagaacggt ggtctctgac
     1801 cacggcctcc gagcgtgccc agggctggcg tcccggcggc ctctggcccg ccgtcggcga
     1861 cggcaacggc cggctctata tgctgatgca cggacacggc gcggagggta ctcacaagga
     1921 cggcggcgat caggtgtgga tattcgatgc ggccaacggc aagcgcatcg gcaagatcga
     1981 gctggcccgc tggggcatcg cgctgagcgt ctccaccggc gagcggcctc agctgatggt
     2041 aagcggcgcc gagccggtca tagacgtcta cgaggctgac ggcacccatg tgagaaccct
     2101 tggcgtggac atgtccacgc cgctcctcgt gcacggcgtt cgctgaacag acctggagat
     2161 cgtcgatgaa ttggttagac aaactatctg agaagtctac gcgcgggctc gcccagcaga
     2221 cgtcccgccg cgggtggctt gcgggtatcg gcaccgtgct ggctggcgcc gcgtcggttc
     2281 ccctgctgcc gatggcaagg gcttccagtg gcgggcagcc gaagctggaa gacgttgctg
     2341 acaccggcga cccgctctcc tgtgattact ggcggcattg ctccatcgac ggttttctct
     2401 gcggctgctg tggcggctct cacaacacct gcccgccggg cacggagatg tcgcccatca
     2461 cctgggtggg tacctgccgc aatcccgttg acggcaagaa ctacgtgatc tcctacaacg
     2521 actgctgcgg aaaaacgccc tgcggacagt gcttctgcaa caccaatcag ggcgaccggc
     2581 ccatgtaccg gcccgataag aacaacgaca tcaactggtg tcttggcacc gaaagcaccg
     2641 tctatcactg ctccacgtcc gtgctgatag ggcttgccgt cgagtgaagg ggcgaacggt
     2701 ctgctttgcc tttctgatcg ccgccgcgcc tcacgccatc ggggacgggc cggtcgacgc
     2761 ggagcgagca cgcctgaact acatgctgaa ctgccaggga tgccatctcc ccgatggttc
     2821 cggggtggaa gacgcggtgc cgcggatgaa cggcttcgtc ggcaacttcc tgcatgtccc
     2881 gggaggcaga gagttcctgg tccgcgtgcc gggatccgca aacgcggcgc tggacgacgc
     2941 ctccctggcc gagctgctca actggattct cgtcaccatg agccccggcg agcttccccc
     3001 gagctgggtg ccctactcgg cgggcgaggt ggccgagctg cgttcggcac cgctcagaga
     3061 agtggagaag gtgagggccg ggctggtccg aagcatccag cagcacctct gaccttggag
     3121 gttctggcaa gcctgaccca aggggaagat ggcggccgaa aactcgtcgg tctcagttcg
     3181 cctcgcgggc caggaacacg accaggctct cgccatccac gaagttagga gcgtcgttgg
     3241 cctggtcggt ggtcatctgc tctgaagcca tgctggcgag cgccgtcggg tagtcgggcc
     3301 agaacagctc cgtgacgccg tcgtaggccc gctgggcggt cctgatgtgg ctctgcacgt
     3361 agcgctccgc gtcctgggtc tgggcggcga tggggccgtg atgggtcagc cagtagtcgt
     3421 ggaactcctc caggctgagc gacggcttcc gatcgaacag gaaggtcgcc ttgaagccgc
     3481 tgtcatgaaa gggctcgccg ctgatgttgt gctcttcgct gcggatcagc acacgggggt
     3541 tgacggcgaa gacctcgtga acgtcctcag cggccgtctc gagcgccgcc atcagcggtg
     3601 tcgggtcgtc gtgctctgcc agccagaact cggccgaggc gcggaagggg gcgagcacca
     3661 gattgccgag ttgctccccc gtcctcagaa actggcgata gcgacgcagc tctggaaaac
     3721 gctcctgaag aaccttgagt gcctgcgctt gattaagctg aaagggtgat tcggccccgt
     3781 cgtcgccgaa cagggcgtag agcttgagca cgatctactc ggtggccagg ccgagcacca
     3841 cggcggtgga gcagttgtac acgctcgagg tcgtgcccat gcaccagttg atgtcgttgt
     3901 tggcgctcgg tcggtagacg ggccggtcgt cctcgttacg gttgcacagg cagcgtccac
     3961 agctgctctt gccgcagcag tcgttgtagg agatcacgta gttcttgccg tccaccggat
     4021 tgcggcaggt gccgatccag gtgagaggcg acatctccgt gcccggcggg caggcgttct
     4081 gggtgccgcc gcagcagctg cacaggaacc cgtcgatggc gcagtagcgc cagtagtcgc
     4141 actgggtggg atctcccgga tcttcggggt tgcccgtgct ttgggggggc acgcggctct
     4201 cgctggcccg tgctacgggc agcaacgggg ttgccgatgc gcccagcagg accgctgagg
     4261 cgccggcgag aaagccccgc cgcgaagtgg ctcgtgcgac cttgcgcgtg gttttctggg
     4321 tgatccggtc cagccagttc atcgcgcggc tccttactcg tagctcacag ggtggaccat
     4381 gtaaggcgtg aaaccgagat cgtagattga tcgcaggtat tcgccgctca tggcgtcgta
     4441 aacgtccagg gtggcctcgg tgcttgccac cagaagatag ggtttctcgt gctgggtgac
     4501 gctgatggaa atggcgggcg cgcgaagctc gatgcgggcc aggcgctcgc gctcgccggt
     4561 gtcgaagagc cagatttcgg gggccgggtc cttgtggctg ccgtcgtagc cgtcgggcgt
     4621 catgcccacc cagagcaatc cgcttttgtg gcctgccgtc cagtgccagc cggccggacg
     4681 ccagttggcg gcacgttcct tctccgtggc cagccaccag gtcgtcatca gctcgggctg
     4741 cgcggcacgg gcatcgatgg gctgaacctg cccgcgatag ctgacgaaat gccagattcc
     4801 gccggccttg gcggacttct cggacagggg gtccgcgtcg atgtcgatga acggctcgct
     4861 ggcctttctg gaaacgggat taccggtctc atccagcgtg atcgagagca ttctcccgtc
     4921 tccgcagagc atgaagaagc tgcgctcgac ggtcggatag atcagcgcgc agccgggcgt
     4981 gctgatctcc gagaccatct tgcgtgagtc cagatcgatg accgccaccg acgtgccggg
     5041 attctgcatg aagaccagca gaaaccgctc gtcctcggtg agcctggaat tgccgagatt
     5101 gatgacggtg ttggcccgcc ggggcggcag gtccacttcg gcgaccacgt cgaggttggc
     5161 gaagtcgtaa atggcgatca cgtcctggcg ctgaccgcgg ctgccgcggc tgtgcagcgt
     5221 ctccccgacg tagaattcct ggcggctgtt ggaaaggtt
//
LOCUS       DBNHOX010000667        14359 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1081895, whole genome shotgun sequence.
ACCESSION   DBNHOX010000667 DBNHOX010000000
VERSION     DBNHOX010000667.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 14359)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14359)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14359
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1081895"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="AC16UZ_14580"
     CDS             <1..354
                     /locus_tag="AC16UZ_14580"
                     /inference="COORDINATES: protein motif:HMM:NF016269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HIR6247962.1"
                     /translation="VGNTTMTQLTIGFQIGGQGFSQIIFFQDERSFREFSTGNFEFGA
                     NASAVAITAGASASASTSGATAGASGGKKDATTAGTAYYKGMKVFTIAKGGLMYEASI
                     GGQKFSYRPAGGDQS"
     gene            complement(408..1853)
                     /locus_tag="AC16UZ_14585"
     CDS             complement(408..1853)
                     /locus_tag="AC16UZ_14585"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6247963.1"
                     /translation="MVDDSGTFPTAGVLGRAFRSGELSPVAVTRELLERIDRLNPALG
                     AVHRLCPERAMAEAAAAERAFSAGEARSPLQGVPYLVKDLFDVAGLPTTAGTRLLEDN
                     VAEADSTAVARLAAAGMVLLGKTVTVQFAYGGAGVNHDHGTPRNPWRQDHYLPGGSSS
                     GSAVAVAAGLAPLALGSDTGGSVRIPASLCGITGLKTTVGRISRAGVYPLSWSLDSVG
                     PLARSVEDAARVYQLLAGADPGDETTLGRPAGDVLAGLEDGLDGLKIGIPENVFWEEV
                     DAEVAEAVLASADVLRSLGARVERMAFPAADGARLLNPRGLVIAAEAYAVNRRFMDDH
                     YDELDPVVATRMIQGKGVAAADYIENLLQWQALRVEAMKQMAGVDALICPSTMIPATP
                     LADCDCDLDRYVERNLAYLRNTSIGNVLNLCGLSVPCGFTAAGLPIGAMICGSPFAEA
                     TVLRIGHAYQRATDWHQRVPPLDWAAGVQSS"
     gene            1983..2483
                     /locus_tag="AC16UZ_14590"
     CDS             1983..2483
                     /locus_tag="AC16UZ_14590"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6247964.1"
                     /translation="MAYTPEQLSDLECIRDAAKRYCRGVDRLDEALMKSAYWPEATDD
                     HGVFVGNAMEFAEMCMVSHLRWRSTSHCIFNHSIELDPDGIHARGEAYNVTFLFQDGA
                     DVLDTWYGRYLDVYEKRGDEWRILERVCVHEGTTTGPVSKMEIDAEKFRHGSFDRPSS
                     GRRIGP"
     gene            2501..3352
                     /locus_tag="AC16UZ_14595"
     CDS             2501..3352
                     /locus_tag="AC16UZ_14595"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR6247965.1"
                     /translation="MNVELELLASGYRLLEGPRTDADGALWFSDVYGGTVNRRDPDGI
                     IEVVVRDRRMVGGLLMHANGGFVMTGPSVVLWRDGQERVLLERDGVRFFNDLHADASG
                     RIYVGSIRADIANLDDEPDPPGEAYRIDLDGSVTELYGGVGISNGIGFSPDGRTLYHV
                     DSTSRGIWAHDLDDAGALSNRRHIAKPAFEKGIPDGLCVDTEGNLWVAHVQGGRVVVL
                     DPGGNHVDEIRVPASWVTSCAFGGSDYRDLYIVSADNTDDKALGGCVWRCRPGAAGHP
                     TPLARVG"
     gene            complement(3357..4358)
                     /locus_tag="AC16UZ_14600"
     CDS             complement(3357..4358)
                     /locus_tag="AC16UZ_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247966.1"
                     /translation="MRHLLTSVTRICDLAEGAFDVAPVPKDTWATGDYVVGEVLDASG
                     YHNVELPNGRLMEAMEGDRVVGAFGARAATLEAVGDWQSIQGDEFDALTPAGLFGRLT
                     SVSPFLPRLMGMRYLGHVHANGVRQTMDGAVTPAPDAALTVPIVLITGTSMSSGKTMS
                     GRLIVRLLSRLGFRVVGAKLTGAARYRDVLSYEDAGAAAVFDFVDEGLPSTVGDPADF
                     AARLERLLSRIAGAAPDVVVAEAGASPLEPYNGKTAIDVLGDRVRFNLLCASDPYAVV
                     GVASAFERTPDLVAGGAANTTAGIELVSKLSGLTAMNLLGKDCHEPLTRMLRDKLGL"
     gene            complement(4369..5541)
                     /locus_tag="AC16UZ_14605"
     CDS             complement(4369..5541)
                     /locus_tag="AC16UZ_14605"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR6247967.1"
                     /translation="MSRADDIRRHLQAEEPEMLELLTALVTLESPSDVPETQGPVLDE
                     LQRVFDGLGYRTIRVPGRASGGHLYARPGRRRSGGNRRWQLLLGHCDTVWPVGTLEKM
                     PLAADGNHLKGPGIYDMKAGLVEMVFALKALEALGLEPEVEPVIFINSDEEIGSREST
                     RYIRALSQRADRALVMEPSLGPRGRLKTARKGVGRFTVTVRGKAAHAGLDPTAGASAI
                     LELSHVIQKLFALNDPERGASVNVGVIDGGVRANVVAPESSAVVDVRVASQEDAERIE
                     RAILSLEAETPGVSLGVVGRIGRPPLEHTPANRQLWRVATDLACELGIQLEEAMAGGG
                     SDGNTTSLFTATLDGLGAVGDGAHADHEHIQVDQLSQRCALLAMLLLAPPLNSGTG"
     gene            complement(5546..6151)
                     /locus_tag="AC16UZ_14610"
     CDS             complement(5546..6151)
                     /locus_tag="AC16UZ_14610"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR6247968.1"
                     /translation="MAGDARATIALDVSESIWERFFCVFPLVVVGTVEPDGTYDLAPK
                     HLAMPMSWKNHFGFVCTPRHRTYQNIERTGEFTVTYARPSQAVLSSLAATPRCEDGSK
                     PITAALPTRPAQTVAGVFLDDGYLFLECRRYRVYDDFGTNSLITGEIVAAGVAEDALR
                     SADEDDQDVIRRAPLLSFLYPGRIAEISASQQLPLPAGFSR"
     gene            6315..6518
                     /locus_tag="AC16UZ_14615"
     CDS             6315..6518
                     /locus_tag="AC16UZ_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247969.1"
                     /translation="MSAGTNQLAIEYKGRQYSGVYSVSGNLMIARIPGISSRSLETAE
                     ADESQARTLLMEILKEADTLGQL"
     gene            6582..7946
                     /locus_tag="AC16UZ_14620"
     CDS             6582..7946
                     /locus_tag="AC16UZ_14620"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6247970.1"
                     /translation="MSQKRLSRSTLLYFGLPDYAIYLAAIPVSLYLPFVYSRDLGLDL
                     TDIGLILMLARISDVITDPLIGYLSDRTQTRFGKRKPWLAGGALVMMVSAWQLFAPMG
                     PVTNWHLFIWSMLLWLGWTMINIPYFAWGAELSDDYNERTRITGWRQAFGYLGNVSVL
                     AIPTLSGELFGYGSLPREGLTIVGFMALVLLPVLMALALWKVPEPEKHRATEANLLVS
                     SRQMFSNGSFMLLFTGFLMLSMGTTWASSMFMLFATFVVDVEGYTQAILLGFYFVQLL
                     SLPLWLQISYRIGKKETWLLGAAIYAIASPGYLMLGSGDVVGLGIVLGLYGIASGNFT
                     AISLSMKADVIDVAALRTGNHVAGSYMAVWSLGQKLFQAIAVGLALPAVAYFGFDPKG
                     ENGPDELLALRLNITVVPMLLYAASVFVIWRYPLTADRLHQLREAFSRRRARREIKQG
                     STGA"
     gene            7969..8433
                     /locus_tag="AC16UZ_14625"
     CDS             7969..8433
                     /locus_tag="AC16UZ_14625"
                     /inference="COORDINATES: protein motif:HMM:NF017771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HIR6247971.1"
                     /translation="MSSDRIETLERQIFDLTRELHELRKANVGDEVPNYTFATLTGEA
                     SLLDLFGSRDTLLAIHNMGQGCRYCTLWADGLNSLLPHLESAMAVVVLSKDAPETQRL
                     FANSRGWQFRLASHGGGNYMAGRSVLLDLERVCARRAGRIELDAPVQLLAAA"
     gene            complement(8493..8789)
                     /locus_tag="AC16UZ_14630"
     CDS             complement(8493..8789)
                     /locus_tag="AC16UZ_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247972.1"
                     /translation="MFGDGSVRIIFTPGHTPGHQTLLISLEKTGPVMLSGDLYHFRAN
                     RRLRRPPNFNVDQEQTLQSMDRLEAVLEETGATLWIEHDKALAETLRKAPAFYD"
     gene            complement(8844..9302)
                     /locus_tag="AC16UZ_14635"
     CDS             complement(8844..9302)
                     /locus_tag="AC16UZ_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6247973.1"
                     /translation="MKRVLVFLLIGFSTPAFAGPRLYVFDCGLLSLDSVEIFNLAEDE
                     TSVRELFVPCYLIEHENGRMLWDGGLPLSYAQAEGPVAIDGGSVSYERSVVDQLADMD
                     ITPEDVDLVAYSHLHWDHAGTANTFIASEVLMQRAEWAAAMGEGGEFVDT"
     gene            complement(9371..9685)
                     /locus_tag="AC16UZ_14640"
     CDS             complement(9371..9685)
                     /locus_tag="AC16UZ_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247974.1"
                     /translation="MTIKSAIYDLLVSRRYDAELETVTEDARRRCVEMLAPRPGGRCA
                     MMDTYWPKPPLRLWLMSWSYAADPKRPGFELLRATCDDFSLEHFPPDVEHGFYLASGT
                     KR"
     gene            complement(9698..10684)
                     /locus_tag="AC16UZ_14645"
     CDS             complement(9698..10684)
                     /locus_tag="AC16UZ_14645"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6247975.1"
                     /translation="MRVLVTGGNRYIGRELVFELARRGHELTVINSHESPLPEGTRRI
                     HADRRLPGEFEAALSAHRDDFDAVFDNTAFTLADLEPMVELFRGRVQHYVFTSSMAVY
                     KRSFVQPLKEDFRRHAADDDDPRKAYGVGKVRCEDLLLAEWQRDAFPATWLRVGHTMG
                     PRSPLPTRDPAFFARLEAGRPILVPGEGFAVLQLVHINDVARCMASLLGNERVRGEGY
                     NVSGSEVASVLGVVHLIGRAMGVQPRVVNVPMEVARAHRPPLLHWGESVSGSAMLSID
                     KALAHLDWEPRFGIEAGYRDAYEWWQREGRGLFEFDFAPEDALVERLGLATE"
     gene            10808..11356
                     /locus_tag="AC16UZ_14650"
     CDS             10808..11356
                     /locus_tag="AC16UZ_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6247976.1"
                     /translation="MTQTNGGLPGLRGVDHIGITAPDFDQAADFLENVLGLEAFYQMG
                     PFGSEETDWMLDMLNVHPRAVIRRVKQFRCGHGPNIELFEYESPDQRREMPKNSDWGG
                     HHITLYVDDIDAAMDHLRAHGVRLMAGGGPPVDDGPEAGLRSCYFLTPWDLQMELISY
                     PDGKAYERGTDRRLWDPRDPAR"
     gene            complement(11353..11742)
                     /locus_tag="AC16UZ_14655"
     CDS             complement(11353..11742)
                     /locus_tag="AC16UZ_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247977.1"
                     /translation="MTIFIRVLAALPALLFLVMGIQWLFAPAEAAAGLEMPLLQGVAA
                     SAQIGDLGSFFFVNGVLMALGQLPGRSHLLYAPAFLIGGAGVFRIAAALLGHAPFAVD
                     FILAEAIMTTILLVAARRLGQPAAAAA"
     gene            complement(11767..12828)
                     /locus_tag="AC16UZ_14660"
     CDS             complement(11767..12828)
                     /locus_tag="AC16UZ_14660"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6247978.1"
                     /translation="MTTHDESTGPEGYRVAEVEAWIAENVQALKPPFEWTRLQGGHSN
                     LTYLLVDGDGNRAVIRRPPQGELLPKAHDMSREWALISALGPTPVPVPAALGFCESPD
                     VTGAWFYVMGFVNGHPLYNSDDTRQWVPREHRRKMAFSFIDVLADLHAVDPDEVGLGE
                     LGKKENYVGRQLKTWYRSWTSSIEAADYDDPRAHELQRYLLENIPEQGPARVVHGDYG
                     LHNCLVGPDCTIAAVVDWEISTLGDPLADLAYALNWWPDPTDTEPVLPEAATAPEGFP
                     TRTELAARYAERTGRDLSKLDYYVGFNRWKSAAIVHGVYARYCEGKKTADEGVDLDEM
                     RGRIDRSLMLSEQAIARLR"
     gene            complement(12876..13328)
                     /locus_tag="AC16UZ_14665"
     CDS             complement(12876..13328)
                     /locus_tag="AC16UZ_14665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR6247979.1"
                     /translation="MSERQLPEGLPDWIVEHIRLYREDPDKGHMWDSTALGGPGVLPT
                     LLLTTRGRRSGEPRILPLIYAEVQGGYAIIASKGGFPTHPAWYLNLVDEPECGVQVGQ
                     DEFRARARTADGEEREAIWAQMEGIYPPYRDYQAATERRIPVVVLERI"
     gene            complement(13410..>14359)
                     /locus_tag="AC16UZ_14670"
     CDS             complement(13410..>14359)
                     /locus_tag="AC16UZ_14670"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR6247980.1"
                     /translation="EAAKEIQRYWFARWALGSPPRSAPDLVGDLRPRLLALGDLILAG
                     LAAAPGRAGSVPPLPGLSESSRRRLSDALSAVEPYPSRLEQVRRSGVLRIGTTGDYPP
                     FSHRVDDDGPLGGADVDLGRLLAHSLGAEARFVPTSWPTLMDDLAAGRYDVAMSGVSR
                     TAARERVAAFTRAYHVGGKTPIARCSERERFSSLAQIDRRGVRVIVNPGGTNELFVDN
                     NLTLADKVLHTDNRSIFQALIEGRADVMITDRIEVRLQVRLHEGVLCATTPRNFNHQE
                     KAYLLPRDAAWHAYVDAWLVSMLAQGVVERTIARHLGED"
BASE COUNT         2515 a         4848 c         4628 g         2368 t
ORIGIN      
        1 gtgggcaaca ccaccatgac acagctcacc atcggctttc agatcggtgg gcagggtttc
       61 agccagatca tcttcttcca ggacgagcgt tccttccgcg agttctccac cggcaacttc
      121 gagttcggag ccaacgccag cgccgtggcc atcaccgcgg gcgcctcggc aagcgcgtcc
      181 acctcgggag cgacggcggg ggcaagcggt ggcaagaagg atgcgaccac cgccggcacg
      241 gcttactaca agggcatgaa ggtgttcacc atcgccaagg gtggtctgat gtacgaagcc
      301 agcatcggcg gccagaagtt ctcctaccga ccggccggcg gcgaccagtc ctgatctgac
      361 taccgctacc agctggcatc gcgcggcggc ccgtcgccgc gcccccgtca actcgactgc
      421 acgcccgccg cccagtccag cggtggcacc cgctggtgcc agtccgtagc ccgctgatag
      481 gcgtgcccga tccgcagcac cgtggcctcg gcgaatggag agccgcagat catcgcgccg
      541 atgggaagcc ccgcggcggt aaatccgcag gggacgctga gcccacagag attcagaacg
      601 ttgccgatgc tggtattgcg cagatacgcc agattgcgtt ccacgtagcg gtcgagatcg
      661 cagtcgcagt cggccagcgg ggtcgccgga atcatcgtcg aggggcagat gagcgcatcc
      721 acccctgcca tctgtttcat cgcttccacg cgcagggcct gccactgcag gagattttcg
      781 atgtagtcgg cggcagcgac gcccttgccc tggatcatgc gcgtggcgac taccggatcc
      841 agctcgtcat agtggtcgtc catgaaacgg cggttcacgg cataggcctc cgccgcgatg
      901 acgagacccc tgggattgag cagcctggcg ccgtccgcgg cggggaaggc cattcgctcg
      961 acgcgcgcgc ccagagatct gagcacatcg gcgctggcca gcacggcttc agccacctcg
     1021 gcatccacct cttcccagaa cacgttttcc ggtattccga tcttcagccc gtctaaaccg
     1081 tcttccaacc cggccagcac gtcgcccgcc gggcggccga gggtggtttc gtcgcccggg
     1141 tcggccccgg cgagcagctg atacacacgt gctgcgtcct cgacgctgcg ggcgagcggc
     1201 cccacggaat ccaggctcca gctgagcggg tacacgcccg cccggcttat cctgcccacc
     1261 gtcgtcttga gccccgtgat cccgcagagg gaggccggga tccggacgga tccccccgta
     1321 tcgctgccga gcgccaacgg cgcgagaccc gccgccaccg ccaccgccga gccgctgctg
     1381 gatccccccg gaaggtagtg gtcctgacgc caagggttac gcggcgtgcc gtgatcgtgg
     1441 ttcacaccgg ccccgccgta ggcgaactgc acggtcaccg tcttgcccag gagcaccatg
     1501 cccgcagcgg caagcctggc cacggcagtg gaatctgcct ccgccacgtt gtcctccagc
     1561 agacgggtcc ctgcggtggt gggcagaccg gcaacgtcga aaagatcttt gaccaggtag
     1621 ggaacaccct gcaggggact ccgcgcctcg ccggccgaaa aagcccgctc cgccgcggcg
     1681 gcttccgcca tggcccgttc ggggcacagc cgatgcacgg cgcccagagc cgggttcagt
     1741 cgatcgatcc gctcgagcag ctcacgggtc accgcaaccg gcgagagctc gccgctgcga
     1801 aatgcccgcc ccagcacacc ggccgtcggg aacgttccag agtcatctac catgctcgac
     1861 ctccggcccc cgggagaagg cggcattcta gcagccacct cggcggggcc cccagatgtg
     1921 gctggctccc ggcgtcgcga cgttgaataa ttgagccgct tagctgatca ggggagagac
     1981 acatggctta cacgcctgag caactgtcgg atctcgagtg catccgggac gccgcgaagc
     2041 gctactgccg cggcgtcgac cgattggacg aggcgttgat gaagtcggcc tactggccgg
     2101 aggccacgga cgatcacggc gtgttcgtcg gcaacgcgat ggagttcgca gagatgtgca
     2161 tggtgagcca cctgcgctgg cgctccacca gccactgcat tttcaaccac agcatcgagc
     2221 tcgaccccga cggcattcac gcgcgaggcg aggcatacaa cgtcaccttt ctgttccagg
     2281 atggcgccga cgtgctcgac acgtggtacg gccgatacct ggacgtctac gagaagcgcg
     2341 gcgacgagtg gcgcatctta gagcgcgtct gcgtccacga gggcaccacc accgggccgg
     2401 tgagcaagat ggagatcgac gccgaaaagt ttcggcacgg atccttcgac cgaccatcat
     2461 ccgggcgccg catcggcccc tgagcacggg aaacaagcct atgaacgtcg agctggaact
     2521 cctggcaagc ggctatcgtc ttctagaggg tccgcgcacc gacgcagacg gggcgctgtg
     2581 gttttcggac gtctacggcg ggacggtgaa ccggcgcgat ccggatggca tcatcgaggt
     2641 cgtcgtgcgc gaccggcgaa tggtcggcgg cctgctcatg cacgcgaatg gcggtttcgt
     2701 gatgaccgga cccagcgtcg tcctctggcg cgacggtcag gagcgcgtgc tgctcgagcg
     2761 cgacggcgtg cgcttcttca acgatctgca cgccgacgcc tcggggcgca tctacgttgg
     2821 ctcgatacgt gcagacatcg ccaacctgga cgacgagccg gacccgccgg gggaggccta
     2881 ccgcatcgac ctggacggca gcgtcacgga gctctatggc ggcgtgggca tctccaacgg
     2941 catcggtttc tctcctgacg gacggactct gtaccacgtc gactcgacca gccggggaat
     3001 ctgggcgcac gatctggacg acgcgggcgc gttgtccaac agacgacata tcgccaagcc
     3061 cgccttcgaa aagggcatcc ccgacggctt gtgcgtggac accgagggaa atctctgggt
     3121 cgcccacgtt cagggcggcc gggtggtcgt tctggacccc gggggcaacc atgtcgacga
     3181 gatccgcgtg cccgccagct gggtcaccag ctgcgccttc ggcggctcgg actatcggga
     3241 cctgtacatc gtgagcgccg acaacacgga cgacaaagcc ctgggcggct gcgtctggcg
     3301 ctgccggccc ggcgccgcgg ggcatcccac cccgctggcg cgcgtcggct gaacggctaa
     3361 agaccgagct tgtcgcgcag catccgggtc aggggctcgt ggcagtcctt gccgagcagg
     3421 ttcatggccg tgaggcccga cagcttcgac accagctcaa tgccggcggt ggtgttcgcc
     3481 gcgccgccgg caaccagatc gggcgtgcgc tcgaaggccg aagcaacacc gaccaccgcg
     3541 taggggtccg atgcgcacag caggttgaaa cggacccggt cgccgagcac gtcgatggcc
     3601 gttttcccgt tgtagggctc cagcggcgag gcgcccgctt cggccaccac cacgtccggc
     3661 gccgcgccag caatccggct gagcaaccgt tccagacgcg cggcaaaatc cgccggatca
     3721 cccaccgtgg acggcaaccc ttcgtccacg aagtcgaaga cggccgctgc gcccgcatcc
     3781 tcgtagctga gaacatcgcg gtaacgggcc gccccggtga gctttgcgcc aacgaccctg
     3841 aaccccaacc ggctcaacag cctcacgatg aggcggccgg acatggtctt ccccgaggac
     3901 atggaggtcc cggtgatgag aacgatgggc acggtcaggg ccgcgtcagg agccggcgtc
     3961 acggccccgt ccatggtctg gcgaacgccg ttggcgtgca cgtgccccag gtagcgcatg
     4021 cccattagcc tcggcagaaa aggagacacg gaggtgaggc ggccgaagag ccccgcgggg
     4081 gtcagcgcgt cgaactcatc gccctgaatg ctctgccagt cacccacggc ttcgagggtg
     4141 gccgctcgag ccccgaaagc gccgacgacc cgatcgcctt ccatggcttc catcaggcgg
     4201 ccattgggca gctcgacgtt gtgatatccg gaggcatcga gtacttcccc gaccacgtag
     4261 tcgccggtcg cccaggtgtc tttcgggacc ggcgccacgt cgaaagcgcc ctcggcaagg
     4321 tcgcagatgc gggtaacgga tgtcagcagg tgtcgcacgc gccgatcctc aaccggttcc
     4381 ggagttcagg ggaggggcaa gcaggagcat ggccagcagc gcgcagcgtt gggacagctg
     4441 gtctacctga atgtgctcgt gatccgcgtg ggcgccatcg cccaccgcgc ccagaccgtc
     4501 cagggtggcg gtaaacagac tggtggtgtt tccgtccgag cccccgccgg ccatggcttc
     4561 ttccagctga atgccgagct cgcatgccag atccgtggcc acgcgccaca actgacggtt
     4621 cgccggcgtg tgctccagcg gtggccggcc gatgcggccg accacgccga gagacacgcc
     4681 cggcgtttcc gcttcgaggc tgagtatcgc ccgctcgatg cgctccgcat cttcctggct
     4741 ggccaccctg acatccacca cggcgctgct ttccggcgcc accacgttgg cgcgcacgcc
     4801 accgtcgatc acgccgacgt tgacggatgc cccccgctcg gggtcgttca gggcaaagag
     4861 cttctgaatc acgtgggaga gctcgaggat ggcgctagcg ccagccgttg gatccagacc
     4921 cgcatgagcc gctttgccgc gaaccgtaac ggtgaatcgg ccaacgccct tgcgcgccgt
     4981 cttcagccgg ccccggggcc ccagggaagg ctccatgacc agcgcccggt cggcccgctg
     5041 cgagagcgcg cggatgtagc gcgtggactc ccggctgccg atctcctcat cggagttgat
     5101 gaaaatcacc ggctccactt cgggctcgag gcccagcgcc tcgagggctt tgagcgcgaa
     5161 gaccatctca accaggcccg ccttcatgtc gtagatgccc ggacctttca ggtggttccc
     5221 atccgcagcc aggggcattt tctccagggt ccctaccggc cagaccgtgt cgcagtggcc
     5281 aaggagcagt tgccatctcc ggttgccgcc cgatcggcgg cggcccggcc gagcatagag
     5341 atggccgccc gaggcgcgcc ccggcacccg gatcgtccgg tagccgagac cgtcgaaaac
     5401 ccgctgcagc tcatcgagca cgggcccctg agtctcgggg acatcggacg gtgactccag
     5461 ggtgaccaga gcggttagca gctccagcat ctccggctct tccgcctgga ggtggcgacg
     5521 aatgtcatcg gcccggctca tccggctacc tggagaatcc cgcgggcagg ggcagctgct
     5581 ggctggccga gatctcggca atccgccccg gatagaggaa gctgagcaac ggcgcccggc
     5641 ggatcacgtc ctgatcgtcc tcgtcggcgc tgcgcagagc atcctccgcc acgccagcgg
     5701 caacgatctc gccggttatt aggctgttgg tgccgaagtc gtcatagacg cgatagcgcc
     5761 gacattcgag aaagaggtaa ccgtcatcga gaaagacgcc ggcaaccgtc tgcgctggcc
     5821 gcgtcggcaa cgcggcggtg atgggtttgc tgccatcctc acatcgcggg gtggccgcga
     5881 ggctcgaaag caccgcctga gaaggccgcg cgtaggtcac ggtgaattcg cccgtccgct
     5941 cgatgttctg atacgtccgg tggcgcgggg tgcagacgaa gccgaagtgg ttcttccagc
     6001 tcatcggcat ggccaggtgc ttgggcgcca ggtcataggt tccgtccggc tcgaccgtgc
     6061 caaccaccac cagaggaaac acgcagaaga accgctccca gatggattcg ctgacatcga
     6121 gcgcaatggt cgctctcgca tcgcccgcca tattggccct cttctcacca ggaaagcgtc
     6181 ccttccacta tcggcagtcg ccccaggccg gaccatacgg ggtttccgag acgcgcccgg
     6241 gcccttcagt gttcgcccaa tcgtgttatc tttcagtgca aatccgacaa acaacgtgac
     6301 ccggtgggga ggctatgagc gcagggacca accagctggc tatcgagtac aaaggcaggc
     6361 agtactcggg cgtatattcc gtgtccggca acctgatgat tgcccggata ccgggaatca
     6421 gctccagaag cctggagacg gcggaagccg acgagtccca ggcccgcacg ctgctgatgg
     6481 aaatcctcaa ggaagcggac accctggggc agctctgagc gccgccgacc tggcgggctg
     6541 cctgacggtg gctgacacgc ccgtttaggc tcgggggtct catgtcgcaa aaacgccttt
     6601 cgcgttccac ccttctctac ttcgggctgc ccgactacgc catctatctg gcggcgatac
     6661 ccgtctcgct ctacctgccc ttcgtctact cgcgggacct gggcctggac ctgacggaca
     6721 tcggcctgat cctcatgctg gcgcgcatca gcgacgtgat caccgacccg ctgatcggct
     6781 atctcagcga tcgcacccag acccggttcg gcaaacgcaa gccgtggctc gccggcggcg
     6841 ccctcgtcat gatggtttcc gcctggcaac tgttcgctcc catggggccc gtcaccaact
     6901 ggcacctctt catctggtcc atgctgctgt ggctgggctg gaccatgatc aacattccct
     6961 acttcgcctg gggcgccgag ctctccgacg actacaacga acgtacgcgc ataacgggct
     7021 ggcgccaggc attcggctac ctcggcaacg tgagcgtgct ggcgattccc accctctccg
     7081 gggagctttt cggctacggt tccctgcccc gggaaggttt gaccatcgtc ggcttcatgg
     7141 ccctggtgct gctgccggtt ctgatggccc tggcgctgtg gaaagtgccg gagccggaga
     7201 agcaccgtgc caccgaggcc aacctgctcg tgagcagccg acagatgttc tcgaacggtt
     7261 ctttcatgct gctgttcacg ggttttctga tgctgtccat gggcaccacc tgggcgtcat
     7321 ccatgttcat gctgttcgcc acgttcgtgg tggacgtgga ggggtacacc caggccatcc
     7381 tgctgggctt ctacttcgtg cagctgctct ccctgccgct gtggctgcag atttcctacc
     7441 ggatcggaaa gaaggagacc tggctgctcg gcgccgccat ctatgccatt gccagtcccg
     7501 gatacctgat gctcggctct ggcgacgtgg tcggcctggg catcgtcctg gggctgtacg
     7561 gcatcgcaag cggcaacttc accgccatct ccctgtccat gaaggcggat gtcatcgacg
     7621 tcgcggccct gcgaacgggc aatcacgtgg cgggttccta catggcggtc tggtcgctgg
     7681 gccaaaagct gttccaggcc atcgccgtgg gcctggcgtt gcccgccgtc gcctacttcg
     7741 gcttcgaccc caagggcgag aacggcccgg acgagcttct cgcgctgcgg ctgaacatca
     7801 cggtggtacc catgctgctc tacgccgcct cggtgttcgt catctggcgc tatccgctga
     7861 ccgccgaccg gcttcaccag ctgcgcgagg ctttttccag gcggcgggcc aggcgggaga
     7921 tcaagcaggg ttccacaggt gcctgattct agaatcgagg aggaaggaat gagcagtgat
     7981 cgaatagaga cgttggaacg tcagatcttc gacctgactc gagagcttca cgaattgcgc
     8041 aaggcgaacg tgggcgacga ggtgcccaac tacacgttcg ccacgctgac cggcgaggcg
     8101 agtctgctgg atcttttcgg gtcccgggac acgctgctcg ccatccacaa catgggccag
     8161 ggatgccgct attgcaccct ctgggcggac gggctgaaca gccttctgcc acacctggag
     8221 tcggccatgg cggtggtggt gctgtccaag gacgcgccgg aaacccagcg ccttttcgcc
     8281 aattcacggg gctggcagtt ccgtcttgct tcccatggag gcggaaacta catggccggg
     8341 cgatctgtac tgctcgatct ggaacgtgtt tgcgctcgcc gggctggacg aatcgagctg
     8401 gacgccccag ttcagctact ggcggcggcc tgagcagctc gacgacggcg gcgcgaacat
     8461 cctggactga accggcgcca cccggcgcgg gctcaatcgt agaaggcggg cgctttgcga
     8521 agggtctccg ccagggcttt atcgtgctcg atccaaagcg tggcaccggt ctcctcgagg
     8581 actgcctcga gccggtccat ggactggagc gtctgctcct ggtcgacgtt gaagttcggc
     8641 ggccgccgca ggcgcctgtt ggcacgaaag tgatacagat cgccagaaag catcaccgga
     8701 cccgtcttct ccaaagagat gagcagcgtc tgatggcccg gcgtgtggcc cggcgtgaag
     8761 atgatcctga cgctgccgtc gccgaacacg tcgtggtcgc cctcgagaaa cgtgacctcg
     8821 gcatcgacca gcttgtcgaa gagctaagtg tcgacgaact cgccgccctc acccatggcc
     8881 gccgcccact cggcccgctg catcaggacc tccgaggcaa tgaaggtgtt ggccgtcccc
     8941 gcatggtccc aatgcaggtg cgagtaggcg acgaggtcga cgtcctccgg ggtgatgtcc
     9001 atgtccgcca gctgatcgac tacggatcgt tcgtagctca ccgatccccc atcgatggcg
     9061 acgggcccct cggcctgggc gtaggacagc ggcaagccgc cgtcccagag catacgcccg
     9121 ttctcatgct caatgaggta gcacggcacg aacagttcgc gtacggacgt ttcgtcctcg
     9181 gcgaggttga agatctccac gctgtcgagg ctaagtaacc cgcagtcgaa gacgtagagc
     9241 cgcggaccag cgaaagccgg cgtcgagaaa ccaatcagca gaaagacgag cactctcttc
     9301 actttgggac gagtctcagg aacgggagcg acaaccatac catcagagca gccggtgcca
     9361 tgggccggag tcagcgcttg gtgccactgg ccaagtaaaa gccgtgctcg acgtcgggcg
     9421 gaaaatgctc gagagagaaa tcgtcgcagg tcgcgcggag gagctcgaag cccggccgtt
     9481 tcggatccgc cgcgtagctc caggacatga gccagagtct gagaggaggc ttcggccagt
     9541 aggtgtccat catcgcgcaa cgcccgccgg ggcgtggcgc gagcatctcc acgcagcggc
     9601 ggcgcgcatc ctcggtcacc gtctccagct ccgcgtcgta gcgccgggag acgagcagat
     9661 cgtagatggc cgacttgatg gtcacgctga tcccgtctca ctcagttgcc aggccgaggc
     9721 gctcgaccaa cgcgtcttcc ggggcaaagt cgaactcgaa cagaccgcgg ccttcccgtt
     9781 gccaccactc gtaagcatcg cggtatccgg cctcgatgcc gaaccggggc tcccagtcga
     9841 ggtgagcgag ggccttgtcg atggagagca tggccgatcc ggacaccgac tcgccccagt
     9901 gaaggagcgg cggacggtga gcgcgcgcca cctccatggg cacgttgacg acacgcggct
     9961 gcacgcccat ggcccgtccg atcaggtgca caacgccgag cacgctggcc acctcgctcc
    10021 cggatacgtt gtagccctca cccctgaccc gctcgttgcc cagcagggac gccatgcagc
    10081 gcgccacgtc gttgatgtgc acgagctgca ggacggcgaa cccctcgccg ggtacgagga
    10141 tcgggcgtcc ggcttccaga cgcgcaaaga acgcagggtc cctcgtgggc agcgggctgc
    10201 gcggccccat ggtatggccg acgcgcagcc acgtcgcggg gaaggcgtcg cgctgccact
    10261 cggcgagcag caggtcctcg cacctgacct tgccgacccc gtaagccttt cggggatcgt
    10321 cgtcgtctgc cgcgtgccga cggaagtcct ccttgagcgg ctggacgaag ctgcgcttgt
    10381 acacggccat ggagctggtg aagacgtagt gttgaacccg accgcggaag agctcgacca
    10441 tgggctcgag atcggccagc gtgaacgcgg tgttgtcgaa cacggcgtca aaatcatccc
    10501 tatgggcaga cagagcggcc tcgaactccc cgggcaaccg gcggtccgca tgaatgcggc
    10561 gggttccttc cggcagcggg gactcgtggc tgttgatgac ggtcagctcg tgaccacggc
    10621 gggccagctc gaagacgagt tcgcggccta tgtaacggtt gccgcccgtg accagaactc
    10681 tcatctagga ccctcccctg cgttcgggct gaagatagca gaggccggcc acagcacgcc
    10741 atgcacaccc ccgggcgttg ctgctaacct tggcggtgtc ggcaacggga gcaaagggga
    10801 gcatgtcatg acccagacaa acggaggcct gccgggactg cgcggcgttg atcacatcgg
    10861 catcacggcg ccggacttcg atcaggcggc ggacttcctg gagaacgttc tcggcctgga
    10921 ggccttctac cagatgggcc ccttcggctc cgaggagacc gactggatgc tcgacatgct
    10981 caacgtccac ccgcgggccg tgatccgccg ggtaaagcag ttccgctgtg gccacggacc
    11041 caacatcgag cttttcgagt acgagtctcc ggaccagcgg cgcgagatgc cgaagaacag
    11101 cgactggggc gggcatcaca tcacgctgta cgtggacgac atcgacgcgg ccatggacca
    11161 cctccgagcc cacggcgtgc gcctgatggc cggtggcggg ccgccggtgg acgacggccc
    11221 cgaggcgggc ctgcgttcct gctacttcct gaccccctgg gacctgcaga tggagctgat
    11281 ctcctacccc gacggcaaag cctacgagcg gggcaccgac cgtcgcctgt gggatcctcg
    11341 ggacccggcc cgctaggccg ccgcagcggc cggttggccc agtcgtcggg cggccaccag
    11401 cagaatcgtc gtcatgatcg cttcggcgag aatgaaatcc accgcgaagg gcgcatgacc
    11461 cagcagggcg gcggcaatgc gaaacacgcc cgccccgccg atgagaaacg cgggggcgta
    11521 gagcagatgg ctccggccgg ggagctgacc tagagccatc agaaccccgt tgacgaagaa
    11581 aaagctcccc agatcgccga tctgcgcact tgccgccacg ccctgcagca gcggcatttc
    11641 aagacccgcg gcagcttccg ccggcgcaaa cagccactga atgcccatga ccaggaacag
    11701 cagcgcgggc aacgcggcga gcacccggat gaatatcgtc atgatcgtct ccctagcatt
    11761 ggccgttcag cgcagccgcg cgatggcctg ctctgaaagc atgagcgacc ggtcgattcg
    11821 acctcgcatc tcgtccagat ccaccccctc gtccgccgtc ttcttcccct cgcaatagcg
    11881 cgcataaacg ccgtgcacga tggcggccga tttccagcga ttgaagccaa cgtagtagtc
    11941 gagcttggag aggtcgcgtc cggtgcgctc ggcgtagcgt gccgcaagct cggtgcgggt
    12001 cgggaatcct tcgggggccg tggccgcttc gggcaacacg ggttccgtgt cggtcgggtc
    12061 tggccaccag ttcagcgcgt aggccagatc cgccagcgga tcgcccaggg tagatatctc
    12121 ccaatccacc accgcggcga tggtgcaatc cggaccaacc aggcaattgt gcaggccgta
    12181 atcgccgtga accacgcggg ccggcccctg ctcgggaatg ttctccagga ggtagcgctg
    12241 cagctcgtgg gcgcgcgggt cgtcgtagtc ggccgcctcg atggacgagg tccaggagcg
    12301 ataccaggtc ttgagctgac ggcccacgta gttctccttc ttccccagtt ctcccaggcc
    12361 cacctcgtcg gggtccacgg cgtgcagatc ggcgagcacg tcgatgaacg agaacgccat
    12421 cttccgacgg tgctcccggg gtacccattg ccgggtgtca tcggagttgt acagcgggtg
    12481 cccgttcacg aagcccatga cgtagaacca ggcgccggtg acgtccgggc tctcgcagaa
    12541 tcccagcgcg gcgggcacgg gcaccggtgt tggcccaagg gccgagatca gggcccactc
    12601 gcggctcatg tcatgggctt tcggcagcag ctcgccctgg ggcggcctgc ggatgacggc
    12661 ccggttgccg tcaccgtcca cgagcaggta ggtgaggttg gagtggccgc cctgaaggcg
    12721 cgtccactcg aaaggcggct tcagggcctg cacgttctcg gcgatccacg cctccacctc
    12781 ggcaacccgg tagccctccg gacccgtgct ctcatcatgc gtcgtcatcg tatctccccc
    12841 cgcgcgacgg cggcgccccc gcgccttccg gcccctcaga tcctttccag caccaccacc
    12901 ggtatccgcc gctcggtggc ggcctggtaa tcccggtagg gcggatagat cccttccatc
    12961 tgcgcccaga tggcttcccg ttcctcgcca tcggcggtgc gggcccgggc gcggaactcg
    13021 tcttggccta cctgaacgcc gcattccggt tcgtccacga gattcagata ccacgccggg
    13081 tgcgtcggaa acccgccttt ggaggcgatg atagcgtagc cgccctgcac ctctgcgtag
    13141 atgaggggga ggatgcgcgg ctcccccgac ctgcgcccac gggtagtcag cagcagcgtg
    13201 ggcagcacgc ccggaccgcc cagcgcggtg gaatcccaca tgtggccctt gtccggatct
    13261 tcccggtaca gcctgatgtg ctcgacgatc caatcgggca agccttccgg cagctgccgt
    13321 tcactcatgt ccgcttcctc ccctttcagt cggctcgatg ctctcatggt gcaccagaga
    13381 agccgtcccc gccagccgtc gccctcgcgt caatcctcgc ccagatgccg ggcgatggtg
    13441 cgctccacca cgccctgagc gagcatgctg acgagccagg cgtcaacgta ggcgtgccat
    13501 gccgcatccc ggggcaggag gtaggctttc tcctggtggt tgaagttacg gggcgtggtg
    13561 gcgcaaagaa ccccttcgtg caggcggacc tgcaggcgca cctcgatacg gtcggtgatc
    13621 atgacatccg cacggccttc tatcagagcc tgaaagatag agcggttgtc tgtgtgcaga
    13681 accttgtcag ccagcgtcag gttgttgtcc acaaagagct cgttggtgcc cccggggttg
    13741 acgatgaccc gaaccccgcg gcggtcgatc tgggcgaggc tcgagaaccg ttcccgctcc
    13801 gagcacctgg caataggcgt cttcccgccg acgtggtacg cgcgggtaaa cgcggcaacc
    13861 cgctcgcgcg ccgccgtccg agacaccccg ctcatggcga cgtcgtagcg tccagcagcc
    13921 agatcgtcca tgagggtagg ccaggaggta gggacgaatc tggcttccgc tccgagcgaa
    13981 tgggccagca agcgaccgag atcgacgtcc gccccgccta agggcccgtc atcgtcaaca
    14041 cggtgcgaaa acggggggta gtctccagtg gtgccgatgc gcagaacgcc cgagcggcga
    14101 acctgctcca gacggctcgg atagggctcc acggccgaca gcgcatcgct cagacgccga
    14161 cgcgacgatt cgctgagccc cgggagcggc ggaaccgacc ctgcgcgacc cggcgccgcg
    14221 gcgagcccgg ccaggatgag gtccccgagg gcaagcagcc ggggccggag atccccgacg
    14281 aggtcggggg ccgagcgcgg cggtgagcca agggcccagc gagcaaacca gtagcgttgg
    14341 atctccttgg ccgcttcga
//
LOCUS       DBNHOX010000668         7658 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_311368, whole genome shotgun sequence.
ACCESSION   DBNHOX010000668 DBNHOX010000000
VERSION     DBNHOX010000668.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7658)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_311368"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="AC16UZ_14675"
     CDS             <1..955
                     /locus_tag="AC16UZ_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247981.1"
                     /translation="DEAATLDLELEAPLGALMGDRYPDSEYRPAQLRYVGKDGSRVAL
                     PVEVRTRGKTRRDEEVCKMPPLRVRFDQAAADGTPFAGQPALKLVTYCRDSDSFDQLV
                     LLEYLAYRTYNLLTPQSHRVRLARISYLERGDRRTTRYGIFLEDWRAVAARNDLTADP
                     VDGGVNVDKLNHDAANRVAVFAYLIGNEDYSLLWPEPDENCCHNIKPLLDRDGRVVPL
                     PYDFDYSGLVNAPYAVAKNPRRKVRQRAYRGLCDTQEGLEAALELTRARREDIYAVFR
                     AQEGLSPAKLKSSMGYLDRFYDLIDQPAQVEKRLRRRCRKS"
     gene            complement(960..1340)
                     /locus_tag="AC16UZ_14680"
     CDS             complement(960..1340)
                     /locus_tag="AC16UZ_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247982.1"
                     /translation="MGYPATKLTVILIVSAIAAPAFANPVQCTQGSLVRSVEVVYSGP
                     DQTLPCEVIYDKTQEGAGIHSLWRASNEAGYCEAQAAAFADKLGAMGWDCGEPAGSGI
                     DVDSGDAPSAADVPAEIPIAPDPA"
     gene            complement(1357..2907)
                     /locus_tag="AC16UZ_14685"
     CDS             complement(1357..2907)
                     /locus_tag="AC16UZ_14685"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR6247983.1"
                     /translation="MSWEEEIRELRKREQLAYQMGGAEKVARQHEFNKLTIRERIAAI
                     SDSGSFQEIGTLAGVGQYDDDGNLIGFTPSNFVFGAAEIDGRPVILSGDDFTVRGGSA
                     DASIIGKRLQAEGLATELRLPHVRLVDGMGGGGSVKTIEMAGRTYIPEVRGWETVVQH
                     LAVAPSVSLALGSVAGIGAARVATSHYSVIVKNSAQMMIAGPALVDWASLGDVSKEEL
                     GASRIHTRNGAIDDEASSEEEAFDMARRFLSYLPTSTDELPPVTDATDDPERTDEALL
                     EIVPKDPRKVYKMHRIIDSVVDEGSFFEIGRRWGRSIITGLARLNGMPVALFAENPMV
                     YGGAWTADASRKLVRFIDLASTFHLPLVHLEDCPGFLIGKQSEESGTIRFGCQALAAL
                     GQAPVPFCCVVVRKAFGVAGASNHKPGSHCVRMAWPSGDWGSLPIEGGIEVAYKADLA
                     QADDYDAHLSRIKERLNRLRSPFRSAEHFEIEQIIDPRETRPALCRWMKLARQALRTD
                     PPTFWFRP"
     gene            complement(2972..3337)
                     /locus_tag="AC16UZ_14690"
     CDS             complement(2972..3337)
                     /locus_tag="AC16UZ_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247984.1"
                     /translation="MFVYQARYAILRALNCNDWRDGHMSADGTWNTVMNTPMGAQNGT
                     MTLATDGGTLTGTLKGPQGEIELKDGTADGDSLAWKADITTPMAMTLEFSATVNGDEM
                     TGNVKLGAFGDATFTGTRA"
     gene            3364..4059
                     /locus_tag="AC16UZ_14695"
     CDS             3364..4059
                     /locus_tag="AC16UZ_14695"
                     /inference="COORDINATES: protein motif:HMM:NF053177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8091 domain-containing protein"
                     /protein_id="HIR6247985.1"
                     /translation="MSIGTLNEGPLHQAIKSLYNGAGAREEVPVGDFVADVFGPDEVI
                     YEIQTGGFGSLKKKLARLVEDHRVVLVHPIAEVRYIVKLPQGEDQQASRRRSPKRGSV
                     AHVLDRLVSIPALLNHPNFELEVLLIEEEELRTFDPRRVRRRDGWRVVQRRLGSVLAS
                     ERFTCAGDLLRMVPGPLPAEFTTEDLAGAMGQPRWLAQKLAYCLREAGEISISGKRGN
                     ALCYRLARSGAVA"
     gene            4115..4792
                     /locus_tag="AC16UZ_14700"
     CDS             4115..4792
                     /locus_tag="AC16UZ_14700"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6247986.1"
                     /translation="MIELYTASTPNGWKASVTLEELGLPYEVRPVDLMAGEQKAPEFL
                     KINPNGRVPAIVDDGFPIFESGAIMIHLAEKTGRLMPADAHGRSLVIQWLMFQMGGVG
                     PMMGQANVFYRAMEEKIQPAIDRYQNECRRLFEVLDGRLRNSEWLADHFSIADIANWC
                     WARLYYWSGVSLDGLDAMQRWMAAMEARPACQRGVEVPNRLELPRDDDPDGIERQALQ
                     SRRVLVR"
     gene            4792..6609
                     /locus_tag="AC16UZ_14705"
     CDS             4792..6609
                     /locus_tag="AC16UZ_14705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR6247987.1"
                     /translation="MALESVIEGLAWDLTGEYSSPDSQGLQADLDRLSELLDRIEALN
                     GQLNGSGAVAAAQQIHRLADQARELLADPRAYASCLLSVNGRDEAALMLQGRLQGYQT
                     RFGDLLKPLELFIDLADDEGIEAFLADPEVAPAEFVVRHARKRRHERLGLAEEQLING
                     LSQEGIHAWGNLYDQLSGTLTCEVLVGNETREMGIAEAAGLLQKPDVATRRNAWRAIN
                     DSWSAHAETCAASLNAIAGWRLELGRRRGREAPVHFLDAPVHMNRISRETLDTLMDVA
                     AGSRPLARRAARLMARAYGLETIGPWDQRAPAPDLGTAPAAGIPYDQALHTIADAYAD
                     VHPSMGEFVRMMHANRWIEGTRGPNKRPGAYCTGFAKSRTPRIYMTYSGGSSDVITLA
                     HELGHALHSWVMRDLPNSQRGYGMALAETASTFGETLVRDALLGSAGSPQAKLDMAWE
                     EMAALVSFVLNIPTRFEFERRFYEARRERPVLPRELNAMMADAWTECFGDVLEEPDPM
                     FWASKLHFYISGISFYNFPYLFGYLFSLGVYARRGDGADFYPRYEGLLRDTGRMTAEA
                     LAAKHLSEDLSRPGFWEATVASLETRVDAFEDLLDELAL"
     gene            complement(6606..>7658)
                     /locus_tag="AC16UZ_14710"
     CDS             complement(6606..>7658)
                     /locus_tag="AC16UZ_14710"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HIR6247988.1"
                     /translation="DKLGRDFRGDPAAALMEILDPAQNREFRDNYLNLPYDLSRVLFI
                     TTANTLEGIPRPLLDRMEVLDLTGYSDLEKQAIARQYLIPRQRSEAGLSEAQLDISDD
                     ALTTMIRRYTREAGVRGLERVIGRLARKRARQVVDGQSLDTPVAADDLAELLGPERFK
                     SDRGRTGLEPGVAAGLAWTEAGGDVLYVEATLTQKDEKVTLTGHLGQVMQESARAARS
                     HLWSAADRLGLDRPAIEESGVHIHVPAGAVPKDGPSAGVTMACALYSAYKGRALRDDV
                     AMTGELTLSGLVLPVGGVKEKILAAHRAGLRRVILPKDNEADLAKLPQTVLDEMQISL
                     AAHLDDVLAVAFAEES"
BASE COUNT         1235 a         2538 c         2518 g         1367 t
ORIGIN      
        1 cgacgaggcg gccacgctcg acctcgagct ggaggccccc ctcggagcgc tcatgggtga
       61 ccgctaccca gacagcgagt accggcctgc gcagctccgc tacgtgggca aggacggtag
      121 ccgcgtcgcg ctgcccgttg aggtccggac acgtgggaaa acccgccggg acgaggaagt
      181 gtgcaagatg ccgccgctgc gtgtgcgctt cgatcaagcc gccgcggacg gtacgccgtt
      241 cgccggtcag cccgcactaa agctggtcac ttactgtcgc gacagcgact ccttcgatca
      301 gctcgtgctg ctcgagtacc ttgcctacag aacctacaac ttgctgaccc cgcagtcgca
      361 tcgggtccgg cttgcgcgca tcagctacct cgagcgtggg gaccggcgaa cgacccgcta
      421 cggcatcttc ttagaggact ggcgggcagt ggcagcccgc aacgacctca ccgccgaccc
      481 ggtcgacggc ggtgtgaacg tcgacaagct caaccacgac gccgccaacc gcgtggcggt
      541 ctttgcgtac ctgatcggca acgaggacta ctcactgctc tggccggaac ccgacgagaa
      601 ctgctgccac aacatcaagc cgctgctcga ccgggatggc cgggtggtgc cgctgcccta
      661 cgatttcgac tattccgggc tcgtcaacgc gccttatgcc gtggccaaga atccgcgcag
      721 aaaagttcgc cagcgagcct accggggtct ttgcgacacc caggaaggtc tcgaggcagc
      781 gctggagctc acgcgtgcgc ggcgcgagga catctacgcg gtcttccgcg cccaggaagg
      841 gttgtcgccg gcaaagctga agtcgtcaat gggctatctc gaccggttct acgacttgat
      901 cgaccagcct gcgcaggtcg aaaaacgcct gcgccgtcgg tgcagaaaaa gctgaacgat
      961 caggcggggt ccggcgcgat gggtatctct gcagggacat ccgcggcgga cggggcgtca
     1021 ccggagtcga cgtcgattcc ggatcccgcc ggctcgccgc agtcccagcc catggcgccg
     1081 agcttgtctg cgaaagcggc cgcctgggcc tcgcagtagc cggcttcgtt ggatgcccgc
     1141 cacagcgagt ggattcccgc gccttcctga gtcttgtcgt agatcacttc gcagggaagg
     1201 gtctggtcgg gaccgctgta gacgacctcg acgctgcgaa ccaggctccc ctgagtgcat
     1261 tgcaccggat tggcgaacgc cggagcggcg atggccgaga cgatgaggat gacggtcagt
     1321 ttcgttgctg ggtatcccat gagttgctcc ttgagatcac ggccggaacc agaaggtcgg
     1381 cggatcggtt ctcagtgcct gccgcgccag cttcatccat cggcacagcg cggggcgggt
     1441 ttcgcggggg tctatgatct gctcgatctc gaagtgctcc gcagaacgga acggcgagcg
     1501 cagccggttg aggcgctctt tgatccggct gaggtgagca tcgtaatcgt cggcctgggc
     1561 gaggtcggcc ttgtaggcca cctcgatgcc gccctcgatg ggaagcgagc cccagtctcc
     1621 cgacggccac gccatgcgta cgcaatggct gccgggtttg tggttggaag cgccagcgac
     1681 cccgaacgcc ttgcgcacca ccacgcagca gaaggggacg ggtgcctggc ccagagcggc
     1741 cagcgcctgg caaccgaagc ggatggttcc gctctcctcg gactgcttgc cgatcagaaa
     1801 tccggggcaa tcctccagat gcaccaacgg caggtggaac gtgctggcca gatcgatgaa
     1861 acgcaccagc ttgcggctcg catccgccgt ccacgcgccc ccgtacacca tcgggttctc
     1921 tgcaaaaagg gccaccggca tgccgttcag ccgggccaga ccggtgatga tggatcggcc
     1981 ccaacgcctg ccgatctcga agaacgaccc ctcgtcgacc accgagtcga tgatccggtg
     2041 catcttgtag accttgcgcg gatccttcgg aacgatctct agcagcgcct cgtccgtccg
     2101 ctcgggatcg tccgtggcgt cggtgacggg cggcagctcg tccgtgctgg tgggcaggta
     2161 ggagagaaat cgccgtgcca tgtcgaacgc ttcttcctcg gaagacgcct cgtcgtcgat
     2221 ggccccgttt cgggtatgga tgcgagaggc gccgagctct tccttgctga catcgccgag
     2281 gcttgcccaa tccaccagtg cgggtcccgc gatcatcatc tgggcgctgt tcttgacgat
     2341 gacggagtag tggctggtcg ccacccgggc agcgccgata ccggccacgg agcccagcgc
     2401 cagagagacc gatggggcaa ccgccaggtg ctgaacgacg gtctcccagc cgcgcacctc
     2461 gggtatgtag gttcggccgg ccatctcgat ggtcttcacg gatccgccgc cgcccatgcc
     2521 gtccaccaga cgcacgtggg gaagccggag ctcggtggcc aggccctctg cctggagccg
     2581 tttgccgatg atcgacgcgt ccgcggagcc gccgcgcacc gtgaagtcgt cgcctgagag
     2641 aatcaccggc cggccgtcga tctccgcggc gccgaatacg aaattggagg gggtgaaccc
     2701 gatgagattg ccgtcgtcgt cgtactgccc gacgccggcc agggtgccga tctcctggaa
     2761 agagccggag tcgctgatgg cggcgatccg ctcgcggatc gtcagcttgt tgaattcgtg
     2821 ctggcgcgcc accttctcgg cgccgcccat ctgataggcg agctgctcgc gcttgcgcag
     2881 ctcgcgaatt tcctcctccc agctcatgtg gtcggcgtcc ggctgcggtg ggcgaagcgc
     2941 cacgcagccg gttcgctcgg gctcggatcc gtcaggcgcg ggttccggta aacgtggcgt
     3001 cgccgaaagc gccgagcttg acgtttccgg tcatctcgtc accgtttacg gtcgcgctga
     3061 actccagcgt catcgccatg ggcgtcgtga tgtcagcctt ccaggccagc gagtcgccgt
     3121 ctgccgtgcc gtccttgagc tcgatctcgc cctggggtcc tttcagcgtg cccgtcaggg
     3181 tgccgccgtc ggtggccagg gtcatggtgc cgttctgggc gcccatgggc gtgttcatca
     3241 cggtgttcca ggtgccgtct gcgctcatgt gtccgtccct ccagtcgttg cagttgaggg
     3301 ctcggagtat ggcatagcgg gcctggtaga caaacattcc ggagcctgag ttaatggccg
     3361 cccgtgagca tcggcaccct caacgaaggg cctctgcacc aggcaatcaa gtcgctctac
     3421 aacggcgccg gcgcccgtga ggaggtgccg gtcggcgact tcgtagccga cgtcttcggc
     3481 cccgacgagg tcatctacga gattcagacc ggcggtttcg gcagcctcaa gaagaaactc
     3541 gctcgcctgg tggaggatca tcgcgtcgtc ctggtccatc ccatcgcaga ggtgcgctac
     3601 atcgtcaagc tgccccaggg ggaagaccag caggccagcc gtcggcgctc gcccaaacgc
     3661 ggcagcgttg cccacgtgct cgaccggctg gtgagcattc ctgcgcttct gaatcatccc
     3721 aacttcgagc tcgaggttct gctgatcgag gaagaggagt tgcggacctt cgatccgcgc
     3781 agggtccgcc gacgggatgg atggcgtgtg gtgcagcgcc gtctgggcag cgtgctcgcc
     3841 tccgagcgtt tcacctgtgc tggcgatctt ctgcgcatgg tgcccgggcc gcttcctgcg
     3901 gagttcacca ccgaggatct cgccggcgcg atgggtcagc cgcgctggct tgcccagaag
     3961 ctggcctact gcctgcgcga ggccggagag atcagcatct ccggcaagcg gggcaacgcc
     4021 ctgtgctacc ggctagcgcg ttcgggcgcc gtggcttgac atgcggcccg ggggcctcga
     4081 atactccttc gcccgcccat cagcgagcat acccttgatc gagctttaca ccgcgtctac
     4141 gcccaacggc tggaaagcgt ccgtcacttt ggaagaactc ggccttccct acgaggtacg
     4201 gcccgtcgac ctgatggccg gcgagcagaa ggccccggag tttctgaaga tcaacccgaa
     4261 cggcagagtt cccgccatcg tggacgacgg ttttccgata ttcgagtccg gcgccatcat
     4321 gattcacctg gctgagaaaa ccgggcgcct catgccggct gacgctcatg gacgttctct
     4381 ggtcatccag tggctgatgt tccagatggg cggcgtcggc cccatgatgg gtcaggcgaa
     4441 cgtcttctat cgggcaatgg aggaaaagat ccagcccgcc atcgaccgct accagaacga
     4501 gtgtcggcgc ttgttcgaag ttctcgatgg ccgtcttcgg aacagcgagt ggctggccga
     4561 ccacttctcg attgccgata tcgccaactg gtgctgggcg cgcctttact actggtcggg
     4621 tgtttccctc gacgggctcg acgccatgca gcggtggatg gccgccatgg aggcgcgtcc
     4681 cgcctgtcag agaggcgttg aggttcccaa ccggctcgag ctgcctcgag acgatgatcc
     4741 ggacgggatc gaacgccagg cactccagtc acgccgcgtg ctggtgcgct gatggccctc
     4801 gaatcggtaa tcgaagggct ggcctgggat ctgaccggcg agtactcgtc gcccgactct
     4861 cagggcttgc aggccgacct cgaccgactc agcgagctgc tcgatcggat cgaggcgctc
     4921 aacgggcagc ttaatggctc tggcgctgtt gcggccgccc agcagattca ccgtctggcc
     4981 gatcaggccc gggagctgct ggcggaccca cgcgcttacg cctcctgcct gctctcggtg
     5041 aacggccggg acgaagccgc cctcatgctg caggggcgtt tgcagggtta ccagacgcgc
     5101 ttcggggatc tgctcaagcc gctggagctg ttcatcgatc tggcggacga cgagggcatc
     5161 gaagcgtttc tggccgaccc ggaggtggcg ccggccgagt tcgtggtgcg ccatgcgcgc
     5221 aagcggcgcc atgagcgtct ggggctggcc gaggaacagc tcatcaacgg cctctcccag
     5281 gagggcattc acgcctgggg caatctctac gatcagctct ccggcacgct cacctgcgaa
     5341 gtgctggtgg gcaacgagac ccgggagatg ggcatcgccg aagcggccgg cctgctgcag
     5401 aagcccgacg tggcgacccg gcggaacgcc tggcgcgcga tcaacgactc gtggtcggcc
     5461 catgccgaaa cctgtgctgc cagtctcaac gccatcgccg gttggcggct ggagctcggg
     5521 cgccggcgcg gccgcgaagc tcccgtacac ttcctggatg cgccggtgca catgaaccgg
     5581 atcagccgcg aaaccctgga taccctgatg gacgtggctg ccggctcgcg gcccctcgcc
     5641 cggcgggcgg cgcggctgat ggcccgtgct tacggcctgg aaaccatcgg tccctgggac
     5701 cagcgcgcgc cggcacccga ccttggtacg gcccccgccg cggggatccc gtatgaccag
     5761 gcgctgcaca ccatcgccga tgcctatgcg gacgtgcatc cctccatggg cgagttcgtc
     5821 cgcatgatgc atgccaaccg atggatcgag gggacccggg gccccaacaa gcgtccgggc
     5881 gcctactgca cgggcttcgc caagtcccgc acgccgcgta tctacatgac ctactccggt
     5941 ggctccagcg acgtcatcac cctggctcac gagcttggcc acgccctgca cagctgggtc
     6001 atgcgggacc tgccgaacag ccagcgcggc tacgggatgg cgctggccga gacggcaagc
     6061 acttttggtg agactctggt tcgggacgcg ctcctcggca gtgccggctc tccccaggca
     6121 aagctcgaca tggcctggga agagatggcg gcgctggtga gcttcgtcct caacatcccc
     6181 acccgtttcg agttcgaacg gcgtttctat gaagcccgcc gggagcggcc cgtgctgccc
     6241 cgggagctca acgccatgat ggcggatgcg tggacagagt gtttcggcga cgtgctcgag
     6301 gagcccgacc cgatgttctg ggccagcaag ctgcacttct acatatccgg catcagcttc
     6361 tacaactttc cctacctgtt cggatacctg ttcagcctgg gcgtctacgc gcggcgcggc
     6421 gacggtgccg acttctaccc ccgttacgaa gggctgcttc gggacaccgg gcggatgacg
     6481 gccgaggcgc tcgcggccaa gcatctttcc gaggacctgt cgcggcccgg tttctgggag
     6541 gccacggtcg catctctcga gacccgggtc gatgctttcg aggatctgct cgacgagctg
     6601 gcgctctagc tttcctcggc gaaggccact gccaggacgt cgtcgaggtg ggcggccagc
     6661 gagatctgca tctcgtccag aaccgtctgc gggagcttgg caaggtcggc ttcgttgtct
     6721 ttgggcagga tgacccgacg cagcccggcg cggtgcgcgg cgaggatctt ctccttcacc
     6781 ccgccgacgg gcagcaccag gccggacagc gtcagctctc cggtcatggc gacgtcatcg
     6841 cgaagggccc tgcctttgta tgccgagtag agcgcgcacg ccatggtgac accggcggaa
     6901 ggcccatctt tcggcaccgc accggctgga acgtgtatgt gcacaccgct ttcctcgatg
     6961 gcgggcctgt ccaggccgag cctgtccgcc gctgaccaca ggtggctgcg ggccgccctg
     7021 gcagattcct gcatcacctg cccgagatgc ccggtgagcg tgaccttctc gtccttctgg
     7081 gtgagcgtcg cttcgacgta gagcacgtcc ccgcccgcct cggtccaggc cagccccgcc
     7141 gcgacgccgg gctccagccc ggtgcgtccg cggtcggatt tgaatcgctc cggtcccagc
     7201 agttcggcca gatcgtcggc tgcgaccggt gtgtccaggc tctggccgtc caccacctga
     7261 cgcgcgcgct ttctcgccag acggccgatg acgcgctcca gcccccgcac gcccgcttca
     7321 cgggtgtagc ggcggatcat ggtggtgagc gcgtcgtctg agatatccag ctgagcctcg
     7381 gaaaggcccg cctcgctgcg ctgtcggggg atcaggtact ggcgcgcaat ggcctgcttc
     7441 tcgaggtcgc tgtagccggt gagatccagc acctccatcc ggtccagcag cggccgcggg
     7501 atcccctcca gggtgtttgc cgtggtgatg aagaggacgc gagacaggtc gtaaggcaga
     7561 ttcaggtagt tgtccctgaa ctcgcggttc tgggcgggat cgagtatctc catcagggcc
     7621 gcggcggggt cgccgcgaaa gtcgcgaccg agcttgtc
//
LOCUS       DBNHOX010000669         2133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_696545, whole genome shotgun sequence.
ACCESSION   DBNHOX010000669 DBNHOX010000000
VERSION     DBNHOX010000669.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_696545"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="AC16UZ_14715"
     CDS             <1..431
                     /locus_tag="AC16UZ_14715"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR6247989.1"
                     /translation="RDYDPSLPPIRADRAQLVQALINIVRNAGQALGETANPSITLRT
                     RAVRQFTIGSTRHRVVLQIEIADNGPGIPEGMVDQIWFPMISGRADGTGLGLAITQSI
                     VGHHNGIIECVSRPGDTRFSVFLPVESNGEHIEQLENSQP"
     gene            428..1828
                     /gene="ntrC"
                     /locus_tag="AC16UZ_14720"
     CDS             428..1828
                     /gene="ntrC"
                     /locus_tag="AC16UZ_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HIR6247990.1"
                     /translation="MRNRVWVVDDDRSIRWVLERAMQQADIPIAAFASADHAMHHLQD
                     ERPVAVLTDVRMPGMDGMEFIGRIHEAYPELPVIIMTAHSDLDSAVNAYQSGAFEYLP
                     KPFDVDEAVAIVKRAMTFAQEEAPPPAPEQETAQEIIGQAPAMQEVFRAIGRLAHSNV
                     TVLIRGASGSGKELVARALHRHSPRAEGEFVALNMAAIPRELVESELFGHEKGAFTGA
                     NSRREGRFEQADGGTLFLDEIGDMPAETQTRLLRVLADGEFYRVGGHSPVKVDVRIIA
                     ATHQDLEVRVRDGSFREDLFHRLNVISVHVPTLAERRTDIPALASHFLEQAAEELGGE
                     PKIIRPDTMEYMMNLSWPGNVRQLENTCRWLTVMASGREIYLADLPPELGSDVVEQDT
                     NDSWEQALEAWADDLLREPGGLPLLDVAMPRFESVVIRAALRRTGGRKREAAELLGWG
                     RNTLTRKIAELGMTEL"
     gene            1832..>2133
                     /locus_tag="AC16UZ_14725"
     CDS             1832..>2133
                     /locus_tag="AC16UZ_14725"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003171120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O-ribose)-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIR6247991.1"
                     /translation="MPLHVVLYQPEIPPNTGNVIRVCANTGARLHLVEPLGFEMDDSR
                     LRRAGLDYHEFACVDVHASLDDALDACGGGRCYAVSTRGSRRFSEITFTDGDVLLFG"
BASE COUNT          402 a          712 c          690 g          329 t
ORIGIN      
        1 ccagggacta cgaccccagc ctgccgccga ttcgggccga tcgcgcgcag ctcgtccagg
       61 cgctgatcaa cattgtcaga aacgccggcc aggccctggg tgagacggcc aacccctcca
      121 tcacgctgag aaccagggca gtcaggcagt tcaccatcgg ctctacccgg catcgggtcg
      181 tgttgcagat cgagatagcc gacaacggac ccggaatacc cgaaggcatg gtggaccaga
      241 tctggttccc gatgatcagc gggcgagccg acggcaccgg tctggggctg gccattaccc
      301 aatccatcgt tggccatcac aacgggatca tcgagtgcgt cagccgcccc ggggacaccc
      361 gcttttcggt attcctgccc gtcgagagca acggcgagca catcgaacaa ctggagaatt
      421 cgcagccgtg agaaatcgag tctgggtcgt cgacgacgac cggtccatac gctgggtgct
      481 ggagcgggcc atgcagcagg ctgacattcc catcgccgct ttcgcctcgg ccgatcacgc
      541 catgcatcac ctgcaggacg agcggcccgt cgccgtcctt accgacgtgc gaatgcccgg
      601 catggacggc atggagttca ttggtcgcat ccacgaggcc tacccggagc tgccggtcat
      661 catcatgacg gcccattccg acctcgacag cgccgtcaac gcctaccaga gcggcgcgtt
      721 cgagtatctg cccaaaccgt tcgacgtgga cgaagccgtc gccatcgtca aacgggccat
      781 gacctttgcc caggaagaag cgccgccgcc ggcgcccgaa caggaaaccg cccaggaaat
      841 catcggccag gcgcccgcca tgcaggaggt cttccgcgcc atcggccggc tggcacactc
      901 caacgtcacg gtcctgatac gcggcgcatc ggggtcggga aaggagctgg tcgcccgtgc
      961 gctgcaccgc cacagccccc gcgcggaggg cgaattcgtc gctttgaaca tggcggccat
     1021 accgagggag ctggtggaat cagagctgtt cggccacgaa aagggcgctt tcacgggcgc
     1081 caactcgcga cgcgaagggc gtttcgagca ggccgacggc ggcaccctgt tcctggacga
     1141 gatcggcgac atgcccgcag aaacccagac ccggctgctg cgcgtgctgg ccgacgggga
     1201 gttctatcga gtgggcggac actccccggt caaggtggac gttcgcatca tcgcagcaac
     1261 ccaccaggac ctcgaagttc gggtgaggga cggcagcttc cgggaggatc tctttcaccg
     1321 gctgaacgtc atcagcgtcc acgtgccgac tctggcggaa cggcgaacgg acattcccgc
     1381 gctggccagc catttcctgg agcaggcggc ggaggaactg ggcggcgagc ccaagataat
     1441 ccgtcccgac acgatggagt acatgatgaa tctgagctgg ccaggcaacg tgcgccagct
     1501 cgagaacacc tgccgctggc tcaccgtgat ggcctccgga cgtgaaatct atctagccga
     1561 cctgccgccg gagctcggct ccgatgtggt cgagcaggac acaaacgaca gctgggaaca
     1621 ggcgctggaa gcctgggccg acgacctgct gcgcgagccc ggcgggctgc cgctgctgga
     1681 tgtggcgatg ccgcgtttcg aaagcgtggt catccgagcc gcgctcaggc gcaccggcgg
     1741 gcgcaagcgc gaggcggccg agctgctcgg ctggggacgc aacacgctca cccggaagat
     1801 tgccgagctc gggatgaccg aactctgagc catgcccctt cacgtcgtgc tgtaccagcc
     1861 ggagattccg ccaaacaccg gcaacgtcat ccgcgtctgc gcgaacaccg gcgcccgtct
     1921 gcacctggtc gagccgctcg gtttcgagat ggacgactca aggctccggc gggcggggct
     1981 cgactatcac gagtttgctt gcgtcgacgt tcacgccagc ctggacgacg cgctcgacgc
     2041 ctgcgggggc ggacggtgct acgcggtgag cacgcgcggt tccagacgct tctcggagat
     2101 cacgttcacc gacggcgacg tgctgctctt cgg
//
LOCUS       DBNHOX010000670         5456 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_375702, whole genome shotgun sequence.
ACCESSION   DBNHOX010000670 DBNHOX010000000
VERSION     DBNHOX010000670.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_375702"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="AC16UZ_14730"
     CDS             <1..608
                     /locus_tag="AC16UZ_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR6247992.1"
                     /translation="ADHEQNASTSTVRLAGSTGANPFACIAAGIAALWGPAHGGANEA
                     VLNMLDEIGDEKHIDKYVKRAKDKDDPFRIMGFGHRVYKNYDPRAKVMQQTCHEVLDE
                     LGVENDPVLRMAQRLEKIALEDEYFVERKLYPNVDFYSGIILKAIGIPTEMFTVIFAT
                     GRTPGWIAHWNEMIGAPYKIGRPRQLYQGATPRDYVPLAER"
     gene            662..2029
                     /gene="selA"
                     /locus_tag="AC16UZ_14735"
     CDS             662..2029
                     /gene="selA"
                     /locus_tag="AC16UZ_14735"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HIR6247993.1"
                     /translation="MPRLRDLPSIDRLLRSPKLAFLVHAHGAETIKQSLRSIQAAWRA
                     TGEVPEWATDPEEYARRLEDQLAGASYRPVFNLTGTMIHTNLGRALLDPEMFRSIEPL
                     VTRPVNLEFDLASGRRGDRDRVVESRLQVLTGAPAATVVNNNAAALMLCLNTAALGRE
                     VIVSRGELIEIGGSFRLPELMERAGCRLVEVGTTNRTHLRDYQAAIGERTGMLLKVHP
                     SNYHISGFTHDVGVAELAALAREHRLPLCVDLGSGTLVDLRKWGLPHEPMPQEILAQG
                     ADLVTFSGDKLLGSVQAGIIVGDSENVGAIKRNPMKRALRPDKLTLAFLDRTLRLYEN
                     PERLAERLPLLRSLTRTLGELENQGEVLARSLETVLGDFDVRLVPSEVQIGSGSLPNQ
                     RIASRAVRITHDDPSEVRRLGERLRRLATPVIGRLHQEAVWLDVRGAEPLDELIEVLH
                     GLAAP"
     gene            2026..3897
                     /gene="selB"
                     /locus_tag="AC16UZ_14740"
     CDS             2026..3897
                     /gene="selB"
                     /locus_tag="AC16UZ_14740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HIR6247994.1"
                     /translation="MIVTLAGHVDHGKTSLVRCLTGQDTDRLEEEKRRGLTIDLGFAY
                     VSGDHEVDPIGFVDVPGHHRFIHNMVAGVASMQYALMVVAADDGPMPQSREHLQILEL
                     LGISQGAIALTKCDRVDQARLREAEDEIRDLTADSFLADAPIFRTSATSNEGISELLS
                     HLQKVNGEFRVDQPPRQFRLAVDRAFTVRGSGLIATGTVHAGSIVNDDELAIFPAAVR
                     ARVRGIRTQSREAKEARAGDRCALNLAGVSLDQVGRGSWLSASSFEGSRDLTLELRVV
                     ADFPRPVKHWTPVHVYHATSHATGRIALLDRDVLAPEESCLADLVLDEPLLAIHGDRV
                     VLRDQSLDLTLGGGRVVHNARPRGRRRAPARLEFLDAFAKQTPEACFEQAVRHAPVPL
                     EEFQRSWDLLPGHLDRVVEASQSRVHDGFAVAEGLWREWADGIVQEVERRHEEQSGLQ
                     GLKANQVGAPVPERFKSELLRELAGAGKLTQRAGHYLPAEHEVALSESEARLLDRISG
                     QLDQIQPPSVGDLAKQIGVPVAELKRQLQPLAGKRRLVRVSETRYYLPAPLSSLVHVV
                     DALSSKGPFTVREFRDAADIGRNVAIEVLEYLDGRGYTKRQGDVRRVVGDPSRVMAT"
     gene            3910..4002
                     /locus_tag="AC16UZ_14745"
     tRNA            3910..4002
                     /locus_tag="AC16UZ_14745"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3944..3946,aa:Sec,seq:tca)
     gene            4029..5159
                     /locus_tag="AC16UZ_14750"
     CDS             4029..5159
                     /locus_tag="AC16UZ_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007946297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6247995.1"
                     /translation="MPGLLPNVDPDGLLEYSVVYTDRALNHMSLAFRQVMRDISESLK
                     SVYGAASAVVVPGSGTFGMEAVARQFATDRKCLVLRNGWFSYRWSQILEMGRMPTETI
                     VLKARQAEPGTQAPFAPAPLDEVVASIAAEKPNVVFAPHVETSCGMILPDDYLREVAN
                     AVHAVGGLFVLDCIASGAIWVDMKEIGVDVLISAPQKGWTGTPCCALVMLGTSALGAM
                     EGATSTSFACDLGKWLEIMRAYENGGHAYHATMPTDGLRILADVMREIEAFGYETARE
                     RQEVLGRRVRGLLEGHGIASVAAEGYQAPGVVVSYTTDPDIQSGRKFADAGLQIAAGV
                     PLQCDEPDDFRTFRLGLFGLDKLADVDRTVASLEDALNGVLP"
     gene            5236..>5456
                     /gene="metX"
                     /locus_tag="AC16UZ_14755"
     CDS             5236..>5456
                     /gene="metX"
                     /locus_tag="AC16UZ_14755"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HIR6247996.1"
                     /translation="MSATQKFTFQGTFAMRRGSISNPTFAFETLGRLNAQRDNAVLIF
                     TGLSPSAHVASSTADPSSGWWEDMVGPDKP"
BASE COUNT          980 a         1823 c         1758 g          895 t
ORIGIN      
        1 acgcagacca tgagcagaat gcctcgacgt ccaccgtcag gctggccggc tccacgggcg
       61 ccaacccctt cgcgtgcatc gctgccggca ttgccgcact gtggggcccc gcgcacggag
      121 gggccaacga agccgtgctc aacatgctcg atgagatcgg cgacgagaag cacatcgaca
      181 agtacgtcaa acgcgccaag gacaaggacg acccgttccg gatcatgggt ttcggccacc
      241 gggtgtacaa gaactacgat ccgcgtgcca aggtcatgca gcagacgtgt catgaggtgc
      301 tcgacgagct cggcgtggag aacgatccgg tgctgcgcat ggctcagcgc ctggagaaga
      361 tcgcgctgga ggacgagtac ttcgtcgagc gcaagctgta ccccaacgtc gacttctact
      421 cggggatcat cctcaaggcc atcggcattc ccaccgagat gttcaccgtt atcttcgcca
      481 ccggccgcac ccctggctgg attgcccatt ggaacgagat gatcggcgcg ccttacaaga
      541 tcggacgacc gcgacagctc taccagggcg cgacgccccg ggactacgtc cccctcgccg
      601 agcgctgaca aaggtcccgg cccgttttcc gcgccgggga ctctccgccg agagatcgac
      661 gatgccacga ctgcgtgacc tgcccagcat cgaccggctg cttcgctccc cgaagctcgc
      721 cttcctggtc cacgcccacg gcgcggaaac catcaagcag tcattgagat caatacaggc
      781 ggcctggcgg gccaccggcg aggtcccgga gtgggcaacg gatcccgagg agtacgcgcg
      841 gcggctggaa gatcagctcg ccggcgcctc ctatcgcccg gtattcaacc tcaccgggac
      901 gatgatccac accaacctag gccgcgctct gctcgatccg gagatgttcc ggtccatcga
      961 gcctctggtg acccggcccg tgaacctgga gttcgatctt gccagcggcc ggcgaggcga
     1021 ccgggatcgg gtggtcgagt cccggctgca ggtgctcact ggtgctccgg ccgccaccgt
     1081 ggtcaacaac aacgcggccg ccctgatgtt gtgcctgaac acggccgcgc tcgggcggga
     1141 ggtgatcgtt tcccgtggcg agctcatcga gatcggcggc agcttcaggc ttcccgagct
     1201 gatggagcgc gccgggtgtc gcctggtgga ggtgggaacc accaaccgca cccaccttcg
     1261 cgactaccag gctgcgatcg gcgagcgaac gggaatgctg ctgaaagtgc atccatccaa
     1321 ctatcacatc agcggtttca cccacgatgt cggcgtcgcg gagctggcgg ctctggcacg
     1381 ggagcaccgg ctgcccctgt gtgtggacct cggcagcggc acgctcgtcg acctccggaa
     1441 gtgggggctg cctcacgaac ccatgcctca agagattctc gcccagggcg cagatctggt
     1501 gacgttcagc ggagacaagc tgctgggctc cgttcaggcg ggcatcatcg tgggcgactc
     1561 ggaaaacgtc ggcgcgataa agcgaaaccc catgaagcgc gctctgcgtc cggacaagtt
     1621 gaccctggcc tttctggacc ggactctcag gctctacgaa aaccccgagc gtctggccga
     1681 aaggcttccc ctcctgcgct cgctcacccg caccctgggc gagctggaaa accagggcga
     1741 agtgctggct cgatcactgg agaccgttct gggcgacttc gacgtgcgcc tggtgcccag
     1801 cgaagtgcag atcggtagcg gaagcctgcc caaccaacgc atcgcgagcc gggcagtgcg
     1861 catcacccat gacgacccct ccgaggtccg caggctcggc gaacgtctgc gtaggctagc
     1921 cacgccggtg atcggcaggc ttcatcagga ggccgtctgg ctggacgttc gaggcgccga
     1981 gccgctcgac gagctcatcg aggtgctgca cgggctcgcc gcgccgtgat cgttaccctt
     2041 gccgggcacg tcgaccacgg caaaaccagc ctggtccgct gcttgacggg gcaggacacg
     2101 gaccggctcg aagaagaaaa gcgccgcggg ctcacgatcg acctgggctt tgcctacgtc
     2161 agcggcgacc acgaggtgga tcccatcggc ttcgtcgacg tgcccggcca ccaccggttc
     2221 atccacaaca tggtggcagg ggtagcgtcc atgcagtacg ccctgatggt cgtcgcggcc
     2281 gacgacgggc ccatgcccca gagccgcgag cacctgcaga ttctggagct gctgggaatc
     2341 tcccagggag ccattgccct gaccaaatgc gatcgggtcg atcaggccag gctccgcgaa
     2401 gccgaagatg agattcgcga tctgaccgct gactccttcc tggcagacgc ccccatattt
     2461 cgaacgtcag ccacgtccaa cgagggcatc tccgagcttc tcagccacct ccagaaggtc
     2521 aacggcgagt tccgcgtgga tcagcccccg cgccaattcc ggctcgccgt cgaccgcgcg
     2581 ttcaccgtca ggggttccgg cctgatcgca actggcaccg ttcacgccgg atccatcgtc
     2641 aacgacgatg agctggcgat ctttcctgcc gccgtgcgcg ctcgggtgag agggatccgt
     2701 acccagagcc gcgaagccaa agaggcccgg gccggggacc gctgcgcgct gaacctggca
     2761 ggcgtctccc tggaccaggt gggtcgcggt agctggctct cagcaagcag cttcgaaggg
     2821 agccgcgacc tcacgctcga gctgcgggtc gtcgccgact ttccgcgccc ggtcaagcac
     2881 tggactcccg tgcacgtcta tcatgccacc tcacacgcca cgggcagaat tgccctcctc
     2941 gatcgggatg tcctggcgcc agaggagagc tgcctggccg acctggtgct cgacgagccc
     3001 ctgctcgcga tccacggcga tcgcgtcgtg ctgcgcgacc agagcctgga tctgaccctg
     3061 ggaggtggcc gggtagtgca caatgcccgt cctcgaggac gtcggcgcgc gcccgcacgg
     3121 cttgaattcc tggacgcgtt cgcaaaacag acgcccgagg cctgcttcga gcaggccgta
     3181 cgccacgcgc ccgttccgct cgaggagttt cagcgcagct gggatcttct gcccggccat
     3241 ctggatcgcg tcgtggaggc ttcacaatcc cgcgtgcacg acggcttcgc cgtggccgag
     3301 ggcctgtggc gagagtgggc cgacggcatc gtccaggaag ttgagcgccg acatgaggag
     3361 cagtccgggc tccagggact aaaagccaac caggtcggcg cgccggtacc cgagcggttc
     3421 aagtccgagc tgctgcgcga gctggccggg gctggaaagc tcactcagcg ggccgggcac
     3481 tacctgcccg cggaacacga ggtcgcgctg agcgaatcag aagcacgact gctcgatcgc
     3541 atttccggtc agctcgatca gatccaacct cccagcgtcg gcgatctcgc caagcagatc
     3601 ggcgtgccgg tggctgagct gaagcgccag ctgcagcctc tcgccggcaa gcggcgcctg
     3661 gtgcgcgtga gcgagacgcg ctactaccta cccgccccgt tgtcgtccct ggtccacgtc
     3721 gtagacgcct tgagcagcaa agggcctttt accgtcaggg agtttcggga cgctgccgac
     3781 atcgggcgca acgtggccat cgaggtgctc gagtatctgg acggccgcgg ctacacaaag
     3841 cgccaggggg acgtccggag ggtggtcggt gacccgagcc gagtgatggc gacatgataa
     3901 actgccttcg gaagagcatc gtccccggtg gggcgcgcgg ccttcaaagc cgttgaggtg
     3961 cgatgacgtg cctggtgggt tcgactccta cctcttccgc cattttcctg ctgactgacg
     4021 aggaacacat gcccggactg ctgcccaacg tcgaccccga cggcctgctc gagtattcgg
     4081 tggtgtacac cgaccgggca ctcaatcata tgtcgcttgc gttccggcag gtcatgcggg
     4141 acatctccga atcactcaag tcggtctacg gcgcggcgag cgccgtggtg gtgcccggca
     4201 gcgggacctt cggcatggag gccgtcgcca ggcaattcgc caccgacaga aaatgtctcg
     4261 tcctgcgtaa cggatggttc agctatcgct ggagccagat cctagagatg ggccgcatgc
     4321 ccacggaaac catcgtgctg aaagcgcggc aggccgagcc gggcacccag gcgcctttcg
     4381 ccccggcgcc cctcgacgag gtggttgcca gcatcgccgc cgagaagccc aacgtcgttt
     4441 tcgcacccca tgtggagacg tcgtgcggca tgatcctgcc cgacgactat ctccgcgagg
     4501 ttgccaacgc cgtacatgcc gtcggcgggc tgttcgtgct cgactgcatc gcttccgggg
     4561 cgatctgggt agacatgaag gagatcggcg tcgacgtgtt gatcagcgca cctcaaaagg
     4621 gctggacggg gactccctgc tgcgctctgg tgatgctggg aacaagcgcc ctcggggcga
     4681 tggagggcgc cacgagcacc agcttcgcct gcgacctcgg caagtggctg gagatcatgc
     4741 gcgcatacga aaacgggggg cacgcatacc acgccacgat gcccaccgac gggttgcgca
     4801 tactcgcaga cgtcatgcgc gaaatcgaag ccttcggtta cgaaaccgcc cgcgagcgcc
     4861 aggaggtctt gggccgccgg gtgcgcgggc tgctcgaagg tcacggcatc gccagcgtgg
     4921 cggccgaggg gtatcaggcc cccggcgtgg tcgtgagcta caccaccgac ccggacattc
     4981 agtcgggacg gaaattcgcc gacgccggtc tgcagatcgc tgcgggagtg ccgcttcagt
     5041 gcgacgagcc ggacgacttt cggacgttcc gcctgggtct gtttgggctg gacaagctcg
     5101 ccgacgtcga ccgaaccgtc gccagcctgg aagacgcgtt gaacggcgtg ctcccctgac
     5161 gatgttagta tccgccctcc gcagctccgc tgggcggatc tgttaccgac aactcatcgg
     5221 tctggcgacg gcaacatgag cgccacgcaa aagttcacat tccagggcac ctttgccatg
     5281 cggcgcgggt ccatcagcaa cccgaccttc gcctttgaaa cgctcggtcg gctgaacgcg
     5341 cagcgcgaca acgcggtgct catcttcaca ggcctgtcgc catcggccca cgtggcatcg
     5401 tcaacagcag acccctcctc cggatggtgg gaggacatgg tgggaccgga caagcc
//
LOCUS       DBNHOX010000671         2441 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_889027, whole genome shotgun sequence.
ACCESSION   DBNHOX010000671 DBNHOX010000000
VERSION     DBNHOX010000671.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_889027"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="AC16UZ_14760"
     CDS             complement(<1..895)
                     /locus_tag="AC16UZ_14760"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6247997.1"
                     /translation="MNISRRWLRAAVCAVSLLAAAAPAGAVVFKIATDAPDGSLWVSS
                     LRSAAEQVVEHTQGRVTFKFYPGGVMGDDKAVMRKIRSGQLHGAVMTVGGLTRTYTDI
                     QLYNMPMAFRSLAEVDHVRANMDQTLLNGLEEHGFVSFGIAEVGFAYALTQERIATLE
                     EARAQKVWVPDGDEGAARSLESFGITPIPLSIADVLGGLQTGLIDSVTVPPVAAIVLQ
                     WHTQLDHVLDLPLLYVYGTLAVSKRQFNKVSDEDQAYMRQVLGELVREVNAQNRQDHE
                     RAVEVLEEQGLAWHAADPVAVA"
     gene            complement(882..1775)
                     /locus_tag="AC16UZ_14765"
     CDS             complement(882..1775)
                     /locus_tag="AC16UZ_14765"
                     /inference="COORDINATES: protein motif:HMM:NF028121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HIR6247998.1"
                     /translation="MKQSRKRGAATRLLNTAVRAALAAALLMLGACTMVMTSVTGSFA
                     EDLSTAILDNPDVAMVRDGAPAYLILIDGLLAQSPDSVELLGQAAMLNSAYAAAFVSE
                     PERSMALHDKALALAERAVCIGIRDGCELRRRDFSDYEAWVARLRAAQVPLAYRLGTT
                     WAGWIQAHSEDFGAIAELGRVKALMAQVVELDEGYDHGGAHLYMGVFETLLPPSLGGK
                     PEIGRAHFERAVALSGGQHMLTKVMFAEQYARLVFDRELHDRLLNEVIETDPVVPGLT
                     LMNVVARQQAQELLESADEYF"
     gene            complement(1882..2241)
                     /locus_tag="AC16UZ_14770"
                     /pseudo
     CDS             complement(1882..2241)
                     /locus_tag="AC16UZ_14770"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(2254..>2441)
                     /locus_tag="AC16UZ_14775"
     CDS             complement(2254..>2441)
                     /locus_tag="AC16UZ_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6247999.1"
                     /translation="GLLLWSSAASGSAEYLDQWGPALGIQLPTLQVPDEMGAVRTLDD
                     LTGDEGLLLVLVRSADW"
BASE COUNT          459 a          845 c          743 g          394 t
ORIGIN      
        1 gggccaccgc gaccggatcg gcagcatgcc acgcaaggcc ctgctcctcg agcacctcca
       61 cggcacgttc gtggtcttgc cggttctggg cgttgacctc gcgaaccagc tcgcccagga
      121 cctgccgcat gtaggcctga tcctcgtccg agaccttgtt gaactgacgc ttgctgactg
      181 ccagcgtgcc gtagacgtac agcaggggaa gatccagcac atgatcgagc tgggtgtgcc
      241 actgcagcac gatggccgcc accgggggca ccgtcacgct atcgatcagg ccggtctgaa
      301 gccctcccag aacgtcggcg atgcttaacg ggatgggcgt gatgccgaaa gattccagcg
      361 acctcgcggc cccttcgtcg ccgtccggca cccacacctt ctgcgcccgc gcttcttcca
      421 gggtggcgat gcgttcctgg gtcagggcgt aggcaaagcc cacttcggcg atcccgaacg
      481 aaacgaagcc atgctcctcg aggccgttaa gtagcgtctg gtccatgttt gcgcgaacgt
      541 gatcgacctc ggcgagggac ctgaacgcca tgggcatgtt gtacagctga atgtccgtgt
      601 aggtgcgggt caggccaccc acggtcatta cggccccatg cagctgcccg gagcggatct
      661 tgcgcatcac cgccttgtcg tctcccatga cgccgcccgg gtagaacttg aaggtgacac
      721 ggccctgcgt gtgctcgaca acctgctccg cagcagacct cagcgagctc acccacaggg
      781 aaccgtccgg cgcatcggtg gcgatcttga acaccaccgc tccggcaggc gctgctgccg
      841 cgagcaggga aaccgcgcat acggccgcgc ggagccatcg tctagaaata ttcatccgcc
      901 gattccagaa gctcctgtgc ctgttgccgt gcaaccacgt tcatgagcgt tagacccggc
      961 acgaccggat ccgtttcgat cacctcgttc agcagacggt catgcagctc gcggtcaaat
     1021 accagccgcg cgtactgttc cgcgaacatc accttggtca gcatgtgctg acctccggac
     1081 aacgcgacgg cccgctcgaa gtgggcgcgc cctatctcgg gcttccctcc cagagaagga
     1141 ggcagcagcg tctcgaacac gcccatgtaa aggtgcgccc cgccatggtc gtagccctcg
     1201 tcgagctcga ccacctgcgc catgagcgcc ttgacccgac cgagctcggc gatggcgccg
     1261 aagtcctcgg agtgggcctg aatccatccc gcccaggtcg tacccagccg gtatgccagg
     1321 ggcacctgag ctgcgcgcaa ccgcgcgacc caagcctcgt agtccgagaa gtcccgcctc
     1381 cgcagttcgc agccgtcccg tatgccgatg catacggcgc gctcggccag ggcaagggcc
     1441 ttatcgtgca gcgccatgct gcgctcgggc tcgctgacga acgccgcggc gtaggcagag
     1501 ttcaacatcg ccgcctggcc cagcagctcg acgctgtccg gcgactgggc cagcaggccg
     1561 tcgatgagga tcaggtaggc cggcgccccg tcacgaacca tcgccacgtc cgggttgtcc
     1621 agaatggccg tggaaaggtc ttcggcgaaa ctgcccgtca ccgaggtcat gaccatggtg
     1681 cacgcgccga gcatgagcag ggccgctgcg agcgccgccc gcaccgccgt gttcaagagg
     1741 cgggtcgcgg ccccgcgttt cctcgattgc ttcatgactg ccgaaatacg ctccaaacgt
     1801 cgccgcctgc cgaaaagcat cggccctggt ctaaggtgcg ggaacgtgca cgcgcattaa
     1861 agccaagccg accgagcaaa atcaacgccc aggttcattc gcagccagcg cggcgcgaac
     1921 ggcctcgtag acttcttcga ccgccggccg gtcccggtac cccgggcgcg cgaatttcag
     1981 cgccagcacg ccgtccggcc ggacgaagat aattccgggc aaaggcacgc caaacgcccg
     2041 gtctcccggc tcgtagtcag gattgcggat ccccagggcc tcgaagtagg cgccgtcctc
     2101 gtcgtgcagc agtgggtagg cgagttcgtg ctcttcgtga aagaaggcaa gcagcagggg
     2161 atgatcatag gtcatgccca cgacgttgac gcccatcgcg gcgaagcgat ccttccactc
     2221 ggccagctcg accagctggc gctgacaata gggtcaccaa tcggccgagc ggacgagaac
     2281 cagaagcagc ccctcatcac ccgtgaggtc gtcaagcgtt cgaacggccc ccatctcgtc
     2341 aggaacctgc agcgtgggca gttggatacc gagcgccgga ccccactgat ccaagtattc
     2401 cgccgacccg gacgcggcag acgaccacag caacaggccc a
//
LOCUS       DBNHOX010000672         5008 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1788718, whole genome shotgun sequence.
ACCESSION   DBNHOX010000672 DBNHOX010000000
VERSION     DBNHOX010000672.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1788718"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="AC16UZ_14780"
     CDS             <1..792
                     /locus_tag="AC16UZ_14780"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6248000.1"
                     /translation="ELLGFPRMDLPQLKEWSYAWVLPFSRGLSLEQEMWAVNKHIELQ
                     HYIFETMQRKRKDPKDDIITRLTRIEIEDVDIGAKRPLTDTEIIGITDHLLIGGNETT
                     TFALSNGLWLLFRNPDVYRALQADRDKIKNFVEEVLRIESPTQGLYRFVTEDCEIGGV
                     PVPKGATLSIRYGAGNHDPGRFPHPDTPELGRKNAGRHLAFGIGEHVCPGATLSRFEQ
                     RWAWEILLERLRNIRPAPGKNDYKHRGGMWVRALEEIYMQFDKAG"
     gene            complement(807..1709)
                     /locus_tag="AC16UZ_14785"
     CDS             complement(807..1709)
                     /locus_tag="AC16UZ_14785"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6248001.1"
                     /translation="MTMTSGKRPDAGRYQDVFFESDDGLDLYARDYPNPDAPLTVLCL
                     HGLTRNSADFADLAAALQPDFRVLAMDQRGRGRSAYDPDPSRYALPTYVKDAFALMDH
                     LALEHVAVIGTSMGGLMAMLMAARQPDRICGIVFNDVGPVVEAEGLRRIQGYVGKGGE
                     IASWADAVALTQANNGAAFPGLSEADWLAFARRLFVENDAGVPVLAYDAKISEPMQAD
                     QTTAVPADLWPVFDAMGEIPLLVVRGALSDILSRETVEEMARRHPGTTAVEVPDRGHA
                     PMLDEPEAMQAVSEFLAALGGKRG"
     gene            1749..3059
                     /locus_tag="AC16UZ_14790"
     CDS             1749..3059
                     /locus_tag="AC16UZ_14790"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6248002.1"
                     /translation="MTEINRMENPVCPASLAEATLADAEVLECPYPTYDLLREQAPVW
                     QDPRTGIFVVTRYEDLRMICTDTERFSNHRPSNDHDKLTGIARKAYELFVEKGWVPGE
                     SLAARDDPEHRQMRSIFNEAFRPKRINALDSEVQALAYRLIDDFIDHGHCNFVKQFAV
                     PLPLIIICRQMGAREEDVWQIKAWTDAWFRGTGFNLTEEEVIWATEMEIQAQHYFQPI
                     FERLRKEPDGTLLSDLVNTEVPGWGRTLNDNELHAEMMQDTFVGGSETTTNALSAGIM
                     LLAQNPGVWERLKAEPEKYLRTFCEEVVRLESPVQGLTRMAKTDIELHGVTIPRGAVI
                     DARFAAGNRDPEHFDNPGEVDLDRPNAASHLGFSSGTHYCLGAPLARRELYWGFKAFL
                     DNVASFRLAPGRNDLRHYPNYALRALSELHIEFERVPSGDRTDI"
     gene            3096..>5008
                     /locus_tag="AC16UZ_14795"
     CDS             3096..>5008
                     /locus_tag="AC16UZ_14795"
                     /inference="COORDINATES: protein motif:HMM:NF012907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HIR6248003.1"
                     /translation="MRKAVMIRCLIGWCAGLLAAAVAQAQTQNIVYFPEWGVHLQPYY
                     VRHIQDSGAADQLTVINYAFVVPAPNAAGDVVCQMDDPVAAYQQLYTAAMSVDGIPDV
                     DDAGQPLRGHFNQFKKLKAEHPHLKVLIAIGGWLGSVWWSDAAATPKSRETFVASCID
                     LFIRGNLPEAGNAGGVGVAAGVFDGFDIDWEYPITGGAGGTHHSADDDVNLTATLAEF
                     RRQLDALPRAAGTDPYLLTMATPGSAFRGDNYQINADQAHVDWFNLMTYDFHGTWENK
                     TGHQTNILTSVNDPSSDAFKLSIDNAVRLYRDTYGVSVEKLVIGAAFYGRGWSGVSTT
                     DDGLYQSGRTAPGIYEDGYNYYRDLAPLMLGDYTLHWDPLALASWLYSPVERTFWTLD
                     EPQSIALKRRYADAFGLGGVMAWEVSGDDEDGTLVSAMRTGDPGAPVQSSNSALDGAV
                     EVTQPASCAISVEGFNQVLGAETTVSAPAKVDQVEFFGNGVSLGFDNFPPWSWAWFNL
                     PAGVHEITAVASDWAGSSTSSTSVLLNVYSEADMALWQVGASYQPGDEVFYDGCIYSA
                     KRNHVGSRVRLPTGGRYWELVTCSDCGGGGGGGGNQPPVIESVTVYPASGLQEGNDVV
                     ITAVATDEDGSIE"
BASE COUNT          892 a         1644 c         1636 g          836 t
ORIGIN      
        1 gagctcctcg gttttccccg catggatctg ccccagctca aggagtggtc ctacgcctgg
       61 gtgctgccgt tttcccgcgg gctgtccctc gagcaggaaa tgtgggctgt gaacaagcac
      121 atcgagctgc agcactacat cttcgagacc atgcagcgca aacggaagga tccgaaggac
      181 gacatcatca cccggctcac ccggatcgag atcgaggacg tggacatcgg cgccaagcgc
      241 ccgctcaccg acacggagat catcggcatc accgatcacc tgctgatcgg cggcaacgag
      301 accaccacgt tcgcgctttc caacgggctg tggctgctct ttcgcaaccc ggacgtctac
      361 cgggcattgc aggccgatcg cgacaagatc aagaacttcg tcgaagaggt gctgcgcatc
      421 gaatccccca ctcagggcct ctaccggttc gtcaccgagg actgcgagat cggcggggtt
      481 cccgttccca agggcgctac cctcagcatc cgctacggtg ccgggaatca cgaccccggg
      541 cgctttccgc acccggacac gccggagctg ggtcgcaaga acgcgggccg gcacctggcg
      601 ttcgggatcg gcgagcacgt ctgcccaggt gccaccctga gccgcttcga gcagcgctgg
      661 gcctgggaga tcctgctcga gcggctgcgc aacatccggc cggcgccggg caagaacgac
      721 tacaagcacc gcggcggcat gtgggtccgc gccctcgagg agatctacat gcagttcgac
      781 aaggcgggat agcgcctgtc aggacctcag ccgcgtttgc cgccgagcgc ggcgagaaac
      841 tcgctcaccg cctgcatggc ttccggttcg tccagcatcg gcgcgtggcc gcgatcgggc
      901 acctccacag cggtggtgcc gggatggcgc cgcgccatct cctcgacggt ttcccgggag
      961 agaatgtccg agagcgcgcc gcgcaccacc agcagcggaa tctcgcccat ggcgtcgaag
     1021 acgggccaga gatcggcggg tacggccgtg gtctggtccg cctgcattgg ctcggagatc
     1081 ttcgcatcgt aggccagcac cggaacgcca gcgtcgttct cgacgaacag gcgccgggcg
     1141 aaggcgagcc agtcggcctc gctcaagcca gggaaagccg cgccgttgtt ggcctgggtc
     1201 agcgccaccg cgtctgccca cgaggcgatc tcgccgccct tacccacgta gccctggatg
     1261 cggcgcagac cttcggcctc caccaccggg ccaacgtcgt tgaaaacgat gccgcagata
     1321 cggtccggct gccgcgcggc catgagcatg gccatgagcc cgcccatgga ggtgccgatg
     1381 acggccacgt gctcgagcgc caggtggtcc atcagggcaa acgcgtcctt cacgtatgtc
     1441 ggcagcgcgt aacggctcgg gtcgggatcg taagcggacc ggccccggcc tcgctggtcc
     1501 atggccagca cgcgaaagtc cggctggagc gccgcggcca gatccgcaaa atctgccgag
     1561 ttgcgggtca gcccgtgcag gcagagcacg gtcagcggcg cgtccgggtt ggggtagtcg
     1621 cgggcgtaaa gatccagccc gtcgtcgctt tcgaaaaaca cgtcctggta gcggcccgcg
     1681 tcggggcgct tgcctgaagt catggtcatg gcagcattag cgtggcgaaa gatgcgcatg
     1741 gtaacggaat gacagaaatc aaccgaatgg agaatccggt ctgcccggcg agcctggccg
     1801 aggccaccct ggcggacgcc gaggtcctcg agtgccccta tcccacctac gaccttctgc
     1861 gcgagcaggc ccccgtctgg caggacccgc gcacgggcat cttcgtggtc acccgctacg
     1921 aggatctgcg tatgatctgc acggacaccg agcgcttcag caaccaccgg ccgagcaacg
     1981 atcacgacaa gctcaccggc atcgcgcgca aggcctacga gctctttgta gagaagggct
     2041 gggtgcccgg cgaatcgctg gcggctcggg acgacccgga gcaccggcag atgcgctcca
     2101 tctttaacga agccttccgc cccaagcgga tcaacgcgct ggattcggag gtgcaggcgc
     2161 tcgcctaccg tctgatcgac gacttcatcg accacggcca ctgcaacttc gtcaagcagt
     2221 tcgccgtgcc cctgccgctg atcatcatct gccggcagat gggcgcgagg gaggaggacg
     2281 tctggcagat caaggcgtgg acggacgcct ggttcagggg caccggcttc aacctcaccg
     2341 aagaggaggt gatctgggcc acggagatgg agatccaggc ccagcactac ttccagccca
     2401 ttttcgagcg gctgcgcaaa gagccggacg gcaccctgct gtcggatctg gtgaacaccg
     2461 aggtgcccgg ctggggcagg acgctcaacg acaacgagct gcacgccgag atgatgcagg
     2521 atacgttcgt gggcggctcg gagaccacca ccaacgcgct ttccgccggc atcatgctgc
     2581 tggcccagaa tccgggcgtg tgggagcggt tgaaggcgga gccggagaag tacctgcgca
     2641 cgttctgcga ggaggtggtc cggctggaga gccccgttca ggggctcacc cgcatggcca
     2701 agacggacat cgagctccac ggcgtcacga tcccccgcgg tgccgtgatc gacgcccgct
     2761 ttgccgccgg caatcgggac ccggagcact tcgataaccc gggcgaagtc gacctggacc
     2821 gccctaacgc ggcttctcat ctgggcttca gctccggcac ccactactgc ctgggggccc
     2881 cgctggcgcg ccgggagctt tactggggct tcaaggcctt tctcgacaac gtcgcctcct
     2941 tccggctcgc ccccggcagg aacgacctgc gccactaccc caactacgcg ttgcgggcct
     3001 tgagcgagct gcatatcgag ttcgagcggg ttccgtccgg cgaccgaact gatatatagt
     3061 caggcatatt gtctttatag ttcgaggtgg gagctatgcg caaggccgtc atgattcggt
     3121 gcctgattgg ctggtgtgcg ggcttgctgg ctgctgcggt tgcgcaggcc cagacgcaga
     3181 acatcgtcta ctttcccgag tggggcgtcc acctgcagcc ctactacgtg aggcatattc
     3241 aggattcggg tgccgccgat cagctcaccg tcatcaacta cgccttcgtc gtgccggcac
     3301 ccaacgcggc cggcgacgtc gtctgccaga tggacgaccc ggtggcggct tatcagcagc
     3361 tctacaccgc ggcgatgagc gtcgacggca tccccgatgt tgacgatgcc ggccaaccgt
     3421 tgcgcggcca cttcaaccag ttcaaaaagc tcaaggccga gcatccgcat cttaaggtgc
     3481 tgatcgccat cggcgggtgg ctcggctcgg tgtggtggtc ggatgcggcg gcaacgccca
     3541 agtcccgcga gacgttcgtg gcgtcgtgca tcgacctctt catccgcggc aacctgcccg
     3601 aggccggcaa cgcgggcggt gtcggcgtgg cggccggcgt cttcgacggc ttcgacatcg
     3661 actgggagta ccccatcacc ggcggggcag gcgggaccca ccacagcgcc gacgacgacg
     3721 tgaacctcac cgcgaccctt gccgagttcc gccggcagct cgatgctttg cctcgcgctg
     3781 ctggcacgga cccatacctg ctcaccatgg ccaccccggg gagcgcgttc cgcggcgaca
     3841 actaccagat caacgccgat caggcgcacg tggactggtt caacctgatg acctacgact
     3901 ttcacggcac ctgggagaac aagacgggtc accagaccaa tatcctgacc tccgtgaacg
     3961 acccctcctc ggatgcgttc aagctgtcga ttgacaacgc cgtgcgcctt taccgcgaca
     4021 cctacggcgt ttccgtggag aagctcgtga tcggcgctgc cttctacggc cgcggctgga
     4081 gcggggtaag cacgacggac gacggccttt accagagcgg acggacggcg cccggcatct
     4141 acgaggacgg ctacaactac taccgcgacc tggcaccact gatgctcggt gactacaccc
     4201 tgcactggga tccgctggcg ctggcctcct ggctttacag ccccgtggag cggaccttct
     4261 ggaccctgga cgagcctcag tcgattgcgc tcaagcggcg ctacgcggat gccttcgggc
     4321 ttggcggggt catggcctgg gaagtgagcg gcgatgacga agatgggacc ctggtaagcg
     4381 cgatgcgaac cggtgacccc ggggctccag tccaatcttc gaattctgcc ttggacgggg
     4441 ccgtcgaagt gacgcagccg gcgagttgcg ccatctccgt ggaggggttc aatcaggtcc
     4501 tcggcgctga gacgaccgtg tcggcgcccg ccaaggtgga tcaggtggag ttcttcggca
     4561 acggggtttc cctgggtttt gacaattttc cgccctggag ctgggcctgg ttcaacctgc
     4621 ccgccggggt tcacgagatt acggcggtcg cctcggactg ggcgggtagc tcgaccagtt
     4681 cgacctcggt attactgaac gtctactccg aagcagacat ggcgctgtgg caggtgggcg
     4741 ccagctacca gccgggcgac gaggtgttct acgacggctg catctacagc gccaagcgca
     4801 accacgtggg ctcccgggtg cgcctgccca cgggcgggcg ctattgggag ctcgtgacct
     4861 gcagcgactg cggcgggggc ggcggtgggg gcggaaatca gccgccggtg atcgagagcg
     4921 tcacggttta tcctgccagc ggattgcagg aagggaatga cgtggtcatt accgctgttg
     4981 caacggacga ggacggctcg attgagag
//
LOCUS       DBNHOX010000673         2962 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_247642, whole genome shotgun sequence.
ACCESSION   DBNHOX010000673 DBNHOX010000000
VERSION     DBNHOX010000673.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_247642"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="AC16UZ_14800"
     CDS             complement(<1..904)
                     /locus_tag="AC16UZ_14800"
                     /inference="COORDINATES: protein motif:HMM:NF022425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsiV family protein"
                     /protein_id="HIR6248004.1"
                     /translation="MSAMSPRRAAATAGLAAALLGQASDAMEAEEPVDLLLKDWYVTE
                     IIVVQRPPVMDFNSAERLLRDRPPALPLAMRTFATDPSQLGKDLDLDLSTRLSLVHPT
                     LALLPPAQDEADVDEGSGAMVAPAIEPVLEPDPLLDVLAMAAALEEQLEARSFQLIPA
                     EELTMSASADRLARRSGFQVLYHGAWLQPVPPRDAPEYLLLQTGARVADRHQLEGVVA
                     VTLGRYLHFQASFSYTEPGMGLEPMTLVLNPDGSGTPARWPVETRPVMIMNQSRRMRS
                     EEVHYLDHPKLGIIVRIDPVPVPEE"
     gene            complement(901..>2962)
                     /gene="mfd"
                     /locus_tag="AC16UZ_14805"
     CDS             complement(901..>2962)
                     /gene="mfd"
                     /locus_tag="AC16UZ_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR6248005.1"
                     /translation="EQIIRNLTELHVGDPVVHIEHGIGRYLGLTTLEIDGAPSEFLAV
                     AYAEDAKLYVPVTSLHLISRYAGAEHAPLHRLGSDAWDKAKRKAAERAHDVAAELLNI
                     YARRASHSSYRFNDPSADYRRFAAQFPFELTPDQGRAIDEVIADLCSERATDRLICGD
                     VGFGKTEVAMRAAFLAVQSNKQVAVLVPTTLLAQQHFETFRDRFADWPVQIEALSRLR
                     SESEVEAIAERMASGRIDVIIGTHKLLNPALSFNDLGLVIIDEEHRFGVRQKERLKAL
                     RAEVDVLTLTATPIPRTLNMAMSGMRDLSIIATPPAKRLSIKTFVQEKRQHVIKEAVT
                     RELMRGGQVFYVHNEVRTIERAAAELAELVPEARIGVGHGQMPKRQLERVMNDFYHRQ
                     LNVLVCTTIIETGIDIPNANTIIMERADKLGLAQMHQLRGRVGRANRQAYAYLLTPHP
                     KAITKDAVKRLEAIEAAGELGVGFTLATHDMEIRGAGELLGEEQSGQIESVGYSLYMD
                     LLDRAVKAIQDGKTPNLNAPLEPIAQEVNLHTPTLIPEHYLPDVHARLILYKRIANAA
                     DPTELDDLQAEMIDRFGLLPEPLKSLFAVTALKLKTQPLGVVRVDLGESGGRIEFSPD
                     TEVDPLAIVNLVQSHPGTYRLDGATMLRINRTMPDFQARITFAGELLDRLAAHTPNRL
                     VANA"
BASE COUNT          477 a          900 c         1020 g          565 t
ORIGIN      
        1 gttcttccgg aacgggcacc ggatcgatgc gcacgatgat gcccagcttc ggatgatcca
       61 ggtaatgaac ctcctcgctg cgcatgcgtc gactctggtt catgatcatc accgggcgag
      121 tttcaacggg ccatcgcgcc ggcgtgcccg aaccgtccgg gttgagcacc aaagtcatcg
      181 gctcgagccc catgccgggc tcggtgtaag agaagctggc ctgaaagtgc aggtaccgcc
      241 cgagggtgac ggccacgacc ccttcgagct ggtggcggtc ggccactctg gcgccggtct
      301 gcagcagcag gtattcgggc gcatcgcgcg gcggaaccgg ttgcagccag gcgccgtgat
      361 agaggacctg gaagccgctt cgccgggcga gccggtcggc gctggcggac atggtcagct
      421 cctcggccgg gatgagctga aagctgcggg cctcgagttg ctcctcgagc gcggcggcca
      481 tggccagcac atcgaggagg gggtcgggtt cgagcaccgg ttcgatggct ggagcaacca
      541 tcgccccgga tccctcgtcg acgtctgctt cgtcctgcgc agggggtagc aaggccaggg
      601 tcggatgcac gaggctcagg cgggtcgaca gatccagatc caggtctttt ccgagctggg
      661 agggatcggt ggcgaacgtg cgcatagcca ggggcaacgc cggcggacgg tcccgcagga
      721 gccgttcggc ggagttgaaa tccatgaccg gggggcgctg cacgacgatg atctccgtga
      781 cgtaccagtc tttgagcaga agatcgacag gttcttcggc ctccatggcg tcggatgcct
      841 gcccgaggag cgcggcagca agacccgccg tggcggccgc tcttcggggg ctcatggcgc
      901 tcatgcgttg gccaccagcc ggttcggtgt gtgtgccgcc aggcgatcta gaagctcgcc
      961 tgcgaacgtg attcgcgcct gaaagtcagg cattgtccgg ttgatcctca gcatcgtcgc
     1021 gccgtccagc cggtacgtcc cgggatggct ctgtacgagg ttcacgatgg ccagcggatc
     1081 gacctcggtg tcggggctga actcgatccg cccgccgctt tcgccgagat ccacgcgaac
     1141 gacgcccagc ggctgggttt tcagcttcaa ggccgtgacg gcaaacaggg atttcagcgg
     1201 ctcgggcagg agcccgaagc gatcgatcat ctccgcctgc agatcgtcta gctccgtcgg
     1261 gtcggcggcg ttggcgatgc gcttgtagag aatcagccgg gcgtgcacgt cgggcaggta
     1321 atgctccggg atgagcgtcg gcgtgtgcag gttgacctcc tgagcgatgg gttccagcgg
     1381 ggcattgagg ttcggcgtct tgccatcctg aatggctttt acggcccggt ccagaaggtc
     1441 catgtagagg gaatatccca cagactcgat ctgtcccgac tgctcttctc caagcagttc
     1501 gcctgcccct cggatttcca tgtcgtgggt ggccagagtg aaaccgacgc cgagctcgcc
     1561 ggccgcctcg atggcttcca ggcgtttgac ggcgtccttg gtgatcgcct tgggatgcgg
     1621 cgtcagcagg taggcgtacg cctgccgatt cgctcggccg acccggccgc gaagctgatg
     1681 catctgcgcc agacccagct tgtcggcacg ctccatgatg atggtgttgg cattcgggat
     1741 gtcgatgccc gtctcgatga tggtcgtgca gacgagcacg ttcagctggc gatggtagaa
     1801 gtcgttcatg acgcgctcga gctggcgctt gggcatctgg ccatgaccga cgccaattct
     1861 ggcctcgggg accagctccg ccagctctgc ggccgccctt tcgatggttc gcacttcgtt
     1921 gtgcacgtag aagacctggc cgccgcgcat cagctcgcgc gtcacggctt ccttgatgac
     1981 gtgctggcgc ttttcctgca cgaacgtttt gattgaaagc cgtttggccg gcggcgtggc
     2041 gatgatggag agatcgcgca tgccgctcat ggccatgttc agcgtgcggg gaatgggggt
     2101 ggccgtcagg gtcaatacgt cgacctctgc tcgcaaagcc ttgagccgct ccttctgacg
     2161 gacgccgaag cggtgctcct cgtcgatgat caccagaccc agatcgttga aactaagcgc
     2221 gggattgagc agcttgtggg tgccgatgat cacgtcgatc cggcccgacg ccattcgctc
     2281 ggcgatggct tcgacctcgc tttccgagcg aagccgtgac agcgcttcga tctgcacagg
     2341 ccagtcggcg aagcggtcgc ggaaggtctc gaaatgctgc tgggccagaa gcgtcgtggg
     2401 cactagcacg gccacctgct tgttgctctg caccgccaga aaggcggcgc gcatggccac
     2461 ctcggtcttg ccgaagccga cgtcgccgca gatgaggcgg tcggtcgccc gctcggagca
     2521 taggtcggcg atgacctcgt cgatggcgcg gccctgatcg ggcgtcagct cgaagggaaa
     2581 ctgtgccgcg aagcggcggt aatcggcgct cgggtcgttg aagcggtagc tggagtggct
     2641 ggcccggcgg gcgtagatgt tcaggagctc ggcggcgaca tcgtgggcac gctctgctgc
     2701 cttgcgcttg gccttgtccc aggcatccga tcccaggcga tgcagcggcg cgtgctctgc
     2761 gccagcgtag cggctgatca ggtgcagcga tgtgaccggc acgtaaagct tcgcgtcttc
     2821 ggcgtaggcc acggcgagaa actcgctcgg cgcgccgtcg atctccaggg tggtcaggcc
     2881 gagatagcga ccgatgccat gctcgatgtg gaccacgggg tcgccaacgt gcagctcggt
     2941 taggttcctg atgatctgtt cc
//
LOCUS       DBNHOX010000674         2049 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1340150, whole genome shotgun sequence.
ACCESSION   DBNHOX010000674 DBNHOX010000000
VERSION     DBNHOX010000674.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1340150"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            795..>2049
                     /locus_tag="AC16UZ_14810"
     CDS             795..>2049
                     /locus_tag="AC16UZ_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR6248006.1"
                     /translation="MAKKYLPAALAVLLPVSAFAQWTNHYPKVEGFGHHVYLEGYELP
                     IMNAGPMDPAPSPGGEQLVFSARGWLWLMDLESGNARRITDSGSMDSRPEWSPSGGHI
                     VFLRDSGSQLSIVSIDMDSGDERVLVDVEAINLDPVFSPDEKYVYYSSAESGPLELWR
                     VSLDTLHREQVTTSDAVRGRPLKRRPLILAGDVVLLYLKKHRGRDFLEVYNTLTDTAT
                     QLVEDRLTAQADMSLSPDRKFLAYTWPYDGGHELRLTSLAAPDTSILLTRSLGMPLAP
                     AFSHDGAYIYFSEANDEHRMELKRISVSGGPVETVAVNEMDWGARVGTLSVRTAIDGQ
                     PAPVRMNVLDANGHPVIPDVGAVRSEGQNGRVFFYSEGAIELTVPAGTVTISAVQGFE
                     TPEAVMEAEVRGNRTTNVTMELEPVW"
BASE COUNT          392 a          610 c          651 g          396 t
ORIGIN      
        1 atatgggcgc gtgcgactcg gcgaccacgg cgacgacatt ggcgatgatc agtagaccaa
       61 tgctgagacg caatgcttta ctggccggcg ttggttgctc ggattcaaaa agactgtaga
      121 tctttgcgcg aacgctgaag gttccggttt caggacgctg agccattcga attctccggg
      181 ttattgaact gctccgccaa ggcaacgaga taatgcgggg cgcaacttca cgtatcgtaa
      241 cttcatcgct cgaccatgag cttccccctc cgccgctcat ctggcggcgt cgagcaagag
      301 gtgggcctgc tcgagaagta tacggctgtc ctcgagttgg ggcgccgatt aatggggatc
      361 gctaactgct gcgccgggct ggatccggtt ttccggggct ggacgcatca ctatgacagt
      421 aagggcaatc cgtcgttgaa agagcatgaa acggaggcta gtcgcctctt tcatcccctc
      481 cgaaaacggc aattagcggc agttgccgta acccctgggt taatcccgct tagaagggtc
      541 tccagcgcgg attcaagcgg catttactag agggacttgg ccgatttcgc cctggagttg
      601 cataggctgg atgggtccaa cctcaaaacc gaacgccaac cattgggagt aggtcctggt
      661 gtgatttccc tcacccggct tttactgttc tgctaaattc aggctgacac cgggcagggc
      721 gctgccagaa aggccctgag aagaagcgat cccgtgcaag gattcctcgc ccgacaccaa
      781 cctggagagt cttgatggcc aaaaaatacc tgccggcggc gctggccgtt cttcttcctg
      841 tctcggcatt cgcgcagtgg acgaatcatt acccgaaggt ggagggcttt ggtcatcacg
      901 tctacctgga aggttacgag ctcccgatca tgaatgccgg gccaatggac ccggcgccgt
      961 cccctggtgg cgagcagctg gtgttctcgg ccaggggctg gctgtggctc atggacctgg
     1021 agtcgggcaa cgcccgtcgc attacggact cgggcagcat ggactcgcgg ccggagtggt
     1081 cgccctccgg cggtcacatc gtctttctgc gcgacagcgg cagccagctg agcatcgtgt
     1141 ctatcgacat ggattccggg gatgagcggg tgctcgtcga cgtcgaggcg atcaacctcg
     1201 acccggtgtt ctctcccgat gaaaagtacg tgtactacag ctctgcggaa tcgggcccgc
     1261 tcgaactctg gcgggtgtcg ctcgacaccc tgcacaggga gcaggtcacg acgtccgatg
     1321 cagtgcgggg acgaccgctg aagcggcgcc cgttgattct cgccggggac gtggtgctgc
     1381 tttatctcaa gaagcacagg ggccgcgatt tcctcgaggt ctacaacacg cttaccgata
     1441 cggccacgca gctggtcgag gaccggctga cggcgcaggc cgacatgtcc ctgtcacccg
     1501 accggaagtt cctggcctac acctggccct acgacggcgg gcacgaattg aggctgacgt
     1561 cgctggccgc gccggacact tccatactgc tgacccggag tctcggcatg ccactggcgc
     1621 ctgcgttcag tcacgacggc gcctatatct atttctcgga ggccaacgac gagcatcgca
     1681 tggagctgaa gcgcatcagc gtgagcggcg gcccggtcga aacggtagcg gtcaacgaga
     1741 tggactgggg cgcccgtgtc ggcaccttat cggtccggac tgccatcgac gggcagcccg
     1801 ccccggtgag aatgaacgtg ctcgacgcca acggtcaccc ggtgatcccg gatgtcggcg
     1861 ccgtgcgctc ggaggggcag aacggcaggg tcttcttcta ctccgaaggc gcgatcgaac
     1921 tcaccgtgcc ggcaggcacg gtgacgatct cggcggtcca gggtttcgag acgccggaag
     1981 ccgtcatgga ggcggaggtt cgcggcaaca ggaccacgaa cgtcacgatg gaactcgagc
     2041 ccgtctggg
//
LOCUS       DBNHOX010000675         1946 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_633574, whole genome shotgun sequence.
ACCESSION   DBNHOX010000675 DBNHOX010000000
VERSION     DBNHOX010000675.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_633574"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="AC16UZ_14815"
     CDS             <1..618
                     /locus_tag="AC16UZ_14815"
                     /inference="COORDINATES: protein motif:HMM:NF039482.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase catalytic
                     domain-containing protein"
                     /protein_id="HIR6248007.1"
                     /translation="VLGVNRGGLGFLADVSPDQIAEKLAEVLKGDYVIEDHFLLNARI
                     VRAGETLGHSPALNDVVVHMGGMARMMEFSLWVDGEFVYDQRSDGLIVASPTGSTAYA
                     LSAGGPIMHPNLDAIVVVPMFPHTLTSRPLVVTGESEIKILAAEDAEVTCDSQVDFAL
                     RSGDEVCISKFDHPLRLVYTLGHSFYESCRSKLDWATRLGGRNND"
     gene            complement(632..1219)
                     /locus_tag="AC16UZ_14820"
     CDS             complement(632..1219)
                     /locus_tag="AC16UZ_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR6248008.1"
                     /translation="MRGGVVLLAAGSASRFGSDKRTHSLPDGRSLLGATIAKYARAFA
                     EIIVVLGPADRALAEELRRQFDPQTVTIVHAQAARLGMGHSLAAGIRAARGWDFAFVA
                     LGDMPFVQPQTLAALGRAMSEAGHDAIVQPFHEGHKGHPVGFGRRHFSALEALTGDEG
                     AREVVKTSASDLVPLEVDDPGVLQDVDTPADLAGD"
     gene            complement(1226..>1946)
                     /locus_tag="AC16UZ_14825"
     CDS             complement(1226..>1946)
                     /locus_tag="AC16UZ_14825"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR6248009.1"
                     /translation="PCGGSLLIVIEPVAANAAYKAHMSHIRDQLAQRRCVKRTVDLKN
                     RSFRTLQVPQHEPLRWEADSQILEHTYGPRYQLFVIGAGMVSKYLAEMAQMLDYQVTV
                     CDPREDLLNDFGIPGVKLVADMPDDAIKAHASDPASAIVALTHDPRIDDMGMMQALTT
                     DAFYVGAMGSARTSASRRERLAALELSEEQIGRLHAPIGLPIGSKTPPEIAVAILAEI
                     TAVRKARAESACAHLAQLSAG"
BASE COUNT          324 a          625 c          645 g          352 t
ORIGIN      
        1 gtgctgggcg tgaaccgtgg tggcctgggt tttctggccg acgtgtctcc ggatcagatc
       61 gctgagaagc tggccgaggt cctcaaaggc gactacgtga tcgaagatca ctttctcctc
      121 aacgcccgca tcgtccgggc tggcgagacg ctcggccatt cgcccgccct caacgatgtg
      181 gtcgtccaca tgggtggtat ggcccgcatg atggagttct cgctctgggt ggacggcgag
      241 tttgtctacg atcaacgctc cgacgggctc atcgtcgcat ccccgacggg ctcgacggcc
      301 tacgcgcttt ccgccggtgg tcccatcatg catccgaacc ttgacgccat cgtggtggtt
      361 cccatgtttc cacacacgct gacgtctcga cccctggtgg tgacgggaga gtcggagatc
      421 aagatcctcg cggccgaaga tgcggaggtc acctgcgatt cacaggtgga tttcgcgctg
      481 cgctccggcg acgaggtatg catatcgaag ttcgaccacc ctctgcggct ggtctacacc
      541 ctgggccaca gcttctacga gtcctgtcgc agcaagctgg actgggcgac ccgactgggc
      601 ggccggaaca acgactgaac cgcccggctg atcagtcccc ggcgagatcg gccggagtgt
      661 ccacgtcctg cagcacgccg gggtcgtcga cctctaaggg aaccagatca ctggctgacg
      721 tcttcaccac ttcgcgggcg ccttcgtcgc cggtgagcgc ttcaagcgcg ctgaagtgcc
      781 ggcggccgaa cccgaccgga tgacctttgt ggccttcgtg aaacggctga acgatggcgt
      841 cgtgccctgc ctcggacatg gcgcggccca aggccgccag ggtctgtggc tggacgaagg
      901 gcatgtcgcc gagagccacg aaggcgaaat cccatcccct ggctgcgcgg attccggccg
      961 caagggaatg acccatgcca agacgcgcag cctgcgcgtg gacgatggtc acggtctgcg
     1021 gatcgaactg ccgtcgcaac tcttcggcaa gggcgcggtc cgcgggcccg agaaccacta
     1081 ttatctcggc gaatgctctg gcgtatttgg ctatcgtggc gccgagcagc gagcggccat
     1141 cgggcaatga atgcgttctc ttgtcggagc cgaagcggct ggcggaaccc gcggccagca
     1201 gcaccacacc accgcgcacg tcgattcagc cggcgctcag ctgggccagg tgtgcgcagg
     1261 cggattcggc ccgcgcctta cgcacggcgg taatctccgc aagaatggcg acggcgatct
     1321 ccggcggcgt cttgctgccg atgggcaggc caatgggcgc gtgcagccgt ccgatctgct
     1381 cctcgctcag ctccagcgcg gcgagtcgct cccgacggct ggccgacgtc cgcgcggagc
     1441 ccattgcccc cacgtagaag gcgtcggtgg tcagcgcctg catcatgccc atgtcgtcga
     1501 ttcgcgggtc gtgggtcaac gcgacgatcg ccgaagcggg atcgctggca tgggccttga
     1561 tcgcgtcgtc gggcatgtcg gccaccagct tcacgcccgg gatgccgaag tcgttcagca
     1621 gatcttccct cgggtcgcag acggtgacct ggtagtccag catctgggcc atctccgcga
     1681 ggtacttcga caccatgccg gcaccgatga cgaaaagctg gtagcggggg ccgtaggtgt
     1741 gctcgagaat ctgggaatcg gcctcccagc gcagcggctc gtgctggggc acttgcaagg
     1801 tccggaagga gcgatttttc agatccaccg ttcgtttaac gcagcggcgc tgagcgagct
     1861 ggtcgcggat gtgcgacatg tgggctttgt aggcggcgtt cgcagccacg ggctcgatca
     1921 cgatgagcag agagccccca cacggc
//
LOCUS       DBNHOX010000676         2222 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_568880, whole genome shotgun sequence.
ACCESSION   DBNHOX010000676 DBNHOX010000000
VERSION     DBNHOX010000676.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_568880"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="AC16UZ_14830"
     CDS             <1..595
                     /locus_tag="AC16UZ_14830"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6248010.1"
                     /translation="DGTRFAVFTDPDGTFYELVELEGADGESSDKPVIGLGRVNVNVS
                     DFERSEAWYRMFGYVATRKLPPTDSAEVAAAMGFDAPYRIDGAVLSHEVDGSQIELVQ
                     WLSPFDPEPAYPPPVSHLGIHRMALLTGDIEADVAALKAQGVKFISGITPCCSGPDSW
                     GSIVLFYDPDGTVVELVEQPFLGTVARAVIWLRRLFE"
     gene            767..>2222
                     /locus_tag="AC16UZ_14835"
     CDS             767..>2222
                     /locus_tag="AC16UZ_14835"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6248011.1"
                     /translation="MRRAILGRALSLLVPVIWLGIGMPGPALAESDSLALEEIVVTAR
                     KREESMQEVGMALSALGAEDLKIRFDADLQTLQNASPNLIVSDIQQGPGSPAAISIRG
                     IGTTDVEKNFDPTVGVVVDGVFIGVNSGAMLKAIDLESVEILRGPQGTLFGRNSIGGV
                     INVTRARPDFDGLSGSLRAGAGNHGDLQLDAYINITATENLAFRLGGAKRESDGWFKN
                     ATLNRDAGEMDYWVISPSVLWQPTESIELYYRYDKTEQEQDANTLHNIAQPDQIFCLY
                     YGQCAQGLKTPQSGKDYTVLQNDDPPYQTYFDTETHILNVSWDINDQYSVDFVYGGFE
                     TDEEVYQDWDSTPLTLYHTDRPATWEQDSFEARLNFAGERLSYTAGAYYWESDYRIDL
                     TSYIGFGAILGVPGAGFDDAIVFPVYQTVIQSTESYAFFLEADYQFADRWTLTVGGRY
                     TDDKKESGVIDALTPELAIKGNPDDPFKASWDEFT"
BASE COUNT          424 a          685 c          709 g          404 t
ORIGIN      
        1 ggacggtacc cggtttgccg ttttcaccga tcccgacggg acgttctatg agctggtcga
       61 actggaaggc gctgatggcg agtcatccga caagcccgtc atcggtctcg gtcgggtgaa
      121 cgtcaacgtg agcgacttcg aacgctccga agcgtggtac cggatgttcg gatacgtagc
      181 cacccgcaag ctgcctccga cggactccgc cgaggtggca gcggccatgg ggttcgatgc
      241 gccctacagg atcgacgggg ccgtgctcag ccacgaggtg gacggctcgc agatcgagct
      301 ggtccagtgg ctttcgccct tcgaccccga gcccgcctac ccgccgccgg tcagccacct
      361 gggcattcat cgcatggcgc tgctcaccgg cgacatcgag gccgacgtgg ctgcgctcaa
      421 ggcccagggt gttaagttca tctccgggat caccccgtgc tgctcggggc cggattcgtg
      481 gggaagcatc gtcctgtttt acgatccgga cgggacggtg gtggagctgg tggagcagcc
      541 cttcctgggc acggtggccc gtgcagtcat ctggttgcgg cgactgttcg agtagccgac
      601 gcaggttttc tgccgccgag cgagtggttt cggcgcgctc agcctcaagt ctataatctg
      661 ccactgcccg ccggtgggcg ggaccaaggg gaacaagcgt tcgcgggtag ccgctgcgcg
      721 gtgtgttcga ataacagaat cggaaactag cctactggag agaatcatga ggagagcaat
      781 cttgggacga gcgctgtcgc tgctcgtacc ggtaatctgg ctcggcatcg gtatgcctgg
      841 gcccgcgctg gccgagtccg actcgcttgc cctggaggag atcgtcgtca ccgcgcggaa
      901 gcgcgaggag agcatgcagg aagtggggat ggccctgagc gcgctcggcg ctgaggatct
      961 gaagatccgc ttcgacgcgg acctgcagac cctgcagaac gcctcgccca acctgatcgt
     1021 cagcgatatt cagcagggtc cgggcagccc cgcggccatc tcgattcgcg gcatcggcac
     1081 cacggacgtg gagaagaact tcgaccccac ggtgggtgtg gtggtcgacg gcgttttcat
     1141 cggggtgaac tcgggtgcga tgctgaaggc catcgacctg gaaagcgtgg agatcctgcg
     1201 cggaccccag ggcactctgt tcggccgtaa ttccatcggc ggcgtcatca acgtgacccg
     1261 cgcgcggccg gacttcgacg gcctgtccgg gtcgctcaga gccggtgcag gcaaccacgg
     1321 cgacctgcag ctcgatgctt acatcaacat cacggctacg gaaaatctcg ccttccgtct
     1381 tggcggcgcc aagcgcgaga gcgacggctg gttcaagaac gccaccctca accgcgacgc
     1441 cggggaaatg gactactggg tgatcagccc cagcgtgctc tggcagccta ccgaaagcat
     1501 cgagctgtat taccgctacg acaagaccga gcaggagcag gacgccaaca cactgcacaa
     1561 cattgcgcag cctgatcaga tcttctgtct gtattacggt cagtgtgccc aggggctgaa
     1621 gacgccccag tcgggtaagg actacacggt gctgcagaac gacgatccgc cctaccagac
     1681 gtacttcgac accgagaccc atatcctgaa cgtctcctgg gacatcaacg atcagtacag
     1741 cgtggacttc gtctacggcg gtttcgagac tgacgaagaa gtctatcagg actgggattc
     1801 gacccccctg accctctacc acaccgaccg gcccgccacc tgggagcagg acagcttcga
     1861 ggccaggctc aacttcgccg gcgagcggct gagctacacg gcgggcgcct actactggga
     1921 gtccgactac cgtatcgatc tgaccagcta catcggcttt ggagcgattc tcggcgtgcc
     1981 gggtgcgggc ttcgatgacg ccatcgtctt ccccgtgtac cagacggtca ttcagtcgac
     2041 ggaatcctat gcgttcttcc tggaggccga ctaccagttt gcagatcgct ggactctgac
     2101 ggtcggagga cgctacacgg acgacaagaa agagagcggc gtgatcgatg cgctgacgcc
     2161 ggaactcgcc atcaagggca atccggatga tccgttcaag gcttcctggg atgaatttac
     2221 gc
//
LOCUS       DBNHOX010000677         5057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_505913, whole genome shotgun sequence.
ACCESSION   DBNHOX010000677 DBNHOX010000000
VERSION     DBNHOX010000677.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_505913"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /gene="rpsT"
                     /locus_tag="AC16UZ_14840"
     CDS             <1..212
                     /gene="rpsT"
                     /locus_tag="AC16UZ_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011336008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR6248012.1"
                     /translation="QNASQRSMVRTYIKRVDAAIQSREYDAASAALNDAIPVIDRMAS
                     KGIMHKNKAARHKSRLNAKVAALKA"
     gene            complement(213..1349)
                     /gene="proB"
                     /locus_tag="AC16UZ_14845"
     CDS             complement(213..1349)
                     /gene="proB"
                     /locus_tag="AC16UZ_14845"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR6248013.1"
                     /translation="MRARLAAARRVVVKIGSALLTDDGAGLATSAMGSWCEQVNRLMD
                     AGVQVLIVSSGAVAEGVSRLGWSSRPVGVHEQQAAAAVGQMGLMQAYERAFAGHARHT
                     AMVLLTHDDLADRERYLNARATLTRLLDLGVIPIVNENDTVATDEIRFGDNDTLAALV
                     TNLLEADLLVILTDTDGLHERDPRKDSQAPLVSRADAADPRLDVMAGSTSGALGRGGM
                     VTKIQAARIAARSGAHTVITSGRIEGVLNRVLEGADVGTLLLASMNPLDARKRWIAGQ
                     LKVKGELELDRGAVEALRRRGVSLLPVGVSAVRGRFVRGDVVRCVDGDGLAIAQGLVN
                     YSSEEAARLLRAGSDEIADRLGYAAEPELVHRDNLVLLSGGSKP"
     gene            complement(1373..2563)
                     /gene="cgtA"
                     /locus_tag="AC16UZ_14850"
     CDS             complement(1373..2563)
                     /gene="cgtA"
                     /locus_tag="AC16UZ_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016713347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HIR6248014.1"
                     /translation="MQFIDEATIRVEAGKGGGGCLSFRREKYVARGGPDGGDGGDGGD
                     VILVADDSLNTLVDFRYQPLYRAGNGQPGSGRNKTGAHGDARLVRIPVGTSVIDDETL
                     ETLGDLTEAGQELLVARGGRRGLGNAHFKSSVNRAPRRTTTGEPGEVRRLRLQLKLLA
                     DVGLLGLPNAGKSTLIAQVSAARPKVADYPFTTLVPSLGVVGAGTDNSFVMADIPGLI
                     QGAAEGSGLGVQFLRHLARTRILLHLVEVAPLDGSDPIDNARLIEAELADYSQALRER
                     PVWLVLSKTDLVTDADLEGLERRFADAFPGRPLFAVSAVTTAGIGRLTDALLAAVAGI
                     RERLAGDEEYAEREAELQARIGEDVLRGAVLAKPGALRGNSEERDEADDDGEDQVQVT
                     YVPE"
     gene            complement(2612..2881)
                     /gene="rpmA"
                     /locus_tag="AC16UZ_14855"
     CDS             complement(2612..2881)
                     /gene="rpmA"
                     /locus_tag="AC16UZ_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020291029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR6248015.1"
                     /translation="MAHKKAGGSTRNGRDSESKRLGVKKYGGQFVAAGNIIVRQRGTK
                     FHPGDNVGCGRDHTLFATADGYVQFLTRGPHKRKFVCVEPATAEA"
     gene            complement(2899..3210)
                     /gene="rplU"
                     /locus_tag="AC16UZ_14860"
     CDS             complement(2899..3210)
                     /gene="rplU"
                     /locus_tag="AC16UZ_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR6248016.1"
                     /translation="MFAVFSSGGKQHRVTEGEVIRVERLAGEPGDEVVFDKVLMVADG
                     DDVSVGQPFVDGGKVTAEVVSSGRGKKIRVIKFKRRKDYMRRQGHRQWYTELKITGIN
                     G"
     gene            3401..4426
                     /locus_tag="AC16UZ_14865"
     CDS             3401..4426
                     /locus_tag="AC16UZ_14865"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR6248017.1"
                     /translation="MPGDPAIATTTTRISADPNDALTPVYDLARDDFAEVNRLIPHQL
                     TSDVELVEEIGRYIVDSGGKRLRPLMVLLGARCCGYQGDDHVRLAAIIEFLHTATLLH
                     DDVVDRSELRRGRATANALWGNAPSVLVGDFLYSRAFQLMVELEDMDIMAILADATNT
                     IAAGEVQQFASIGNFAIDEAQYMEIIRCKTALLFQAATHTAGVLSSEDPDEVAALKNF
                     GLHFGLAYQLVDDWLDYEGDSSTLGKNVGDDLAEGKLTLPLIHALANAPDADANVVRE
                     AVSARSAERLAEVLQVVRSSGALSYTRSAATRQCARAVECLTGLPDNEYRDALETLAN
                     FCLDRLR"
     gene            4429..>5057
                     /locus_tag="AC16UZ_14870"
     CDS             4429..>5057
                     /locus_tag="AC16UZ_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6248018.1"
                     /translation="MRPAPSILLACLCFLPFASALAAAPALEQCELSGSHGVGRVEAV
                     CGRLPRPENPEQPDGARLDLFIAKIPSLSPEPAPDAFTIINGGPGASSVSLFVDLAQA
                     FSGVLRERDIVLVDQRGTGRSNALECPELEKAAEDYSIEAVQEATRACLESLAGDPRF
                     YTTSQAVQDLEAVRAALGYERLNVYGVSYGTRVSMHYLRRYPDRVRTLIL"
BASE COUNT          870 a         1799 c         1532 g          856 t
ORIGIN      
        1 gacaaaatgc cagccagcgt tccatggtgc gcacctacat caagcgcgtc gacgcggcca
       61 tccagagccg ggagtacgac gcggccagcg cagccctgaa cgatgcgata cccgtcatcg
      121 accggatggc ttccaagggc atcatgcaca agaacaaggc agcccggcac aagtcgcgcc
      181 tgaacgccaa agtcgccgcc ctgaaagcct gatcagggct tcgacccacc ggagagcagc
      241 accagattgt cccggtgcac gagctccggc tccgccgcat accccaacct gtcggcaatc
      301 tcgtcagacc cggcccgcag caatcgggca gcctcctcgc tgctgtaatt gaccagcccc
      361 tgagcgatcg ccagcccgtc gccgtccacg cagcggacca cgtcaccgcg cacgaaccgt
      421 ccccgcacgg cagacacccc tacgggcaga aggctgaccc cgcggcggcg cagcgcctcg
      481 accgcgcccc tgtcgagctc cagctcgccc ttcaccttga gctgaccggc gatccacctt
      541 ttgcgcgcat cgagcgggtt catcgaagcg agcagcagcg tgcccacatc ggccccttcg
      601 aggacgcggt tcagcacgcc ctcgattctg ccgctggtaa tcacggtgtg ggccccggag
      661 cgtgcggcaa tacgggccgc ctggatcttg gtcaccatgc cgcctcgccc caacgcgccc
      721 gaagtgctgc ccgccatcac gtccaggcgc ggatcggccg catcggccct ggataccagc
      781 ggggcctggg aatccttgcg cggatcgcgc tcgtgcaggc catcggtgtc cgtgaggatc
      841 accagcagat cggcttccag cagattggtg accagcgccg ccagagtatc gttgtccccg
      901 aagcggatct cgtccgtggc gacggtatcg ttctcgttga cgatggggat gacgcccagg
      961 tcgagcaggc gggtcagggt ggcccgggcg ttgaggtagc gctcgcggtc cgccagatcg
     1021 tcgtgggtca gcaggaccat ggcggtgtgt cttgcgtgcc cggcaaaagc gcgctcgtaa
     1081 gcctgcatga gacccatctg ccccacggcc gctgcagcct gctgctcgtg cacgcccaca
     1141 ggccgcgagc tccagcccag acggctgacg ccctcagcca ccgctccgga ggacacgatg
     1201 agcacctgca ctcctgcgtc catcagccgg ttcacctgct cgcaccacga ccccatggcc
     1261 gatgtggcca gcccagcgcc gtcgtcggtc agcagcgcgc tgccgatctt gaccacgacc
     1321 cgtcgggcgg cggccagccg cgcacgcacc tcgtcactcg gaatatccct gctcattcgg
     1381 gaacgtacgt tacctgaacc tgatcctcgc cgtcatcgtc cgcttcgtcc ctctcctcgg
     1441 agttcccccg cagcgcaccg ggctttgcga gcacggcgcc gcgcagcacg tcctccccga
     1501 tccgggcctg cagctccgcc tcccgttcgg cgtactcttc gtcaccggca agacgctcgc
     1561 gaattccggc cacggcagcc agcagcgcgt ccgtcagcct gccgattcca gcggtggtga
     1621 cggccgacac ggcaaagagg ggacgcccgg gaaaagcgtc ggcgaaacgc cgctcaaggc
     1681 cttcgagatc ggcatccgta accaggtccg tcttgctgag cacgagccag acgggccgtt
     1741 cgcgcagtgc ctgactgtag tcggccagct ccgcttcgat gagcctcgcg ttgtcgatgg
     1801 ggtcggagcc gtccagcggc gcgacctcca ccaggtgcag cagaatcctg gtacgcgcaa
     1861 gatggcgcag aaactggacc ccgaggccgg atccttccgc agcgccctga atcaatccgg
     1921 gaatgtccgc catgacgaag ctgttgtcgg taccggcgcc cacgaccccc aggctgggaa
     1981 cgagcgtggt gaagggataa tccgccacct tgggccgcgc cgcggagacc tgcgcgatca
     2041 gcgtggactt gcccgcgttg ggcagcccca gcagcccgac gtcggcgagc agcttgagct
     2101 gcaggcgcag ccggcggacc tcaccaggct cgccggtggt cgttctccgc ggggcccgat
     2161 tgacactgga tttgaagtga gcgttgccca gaccccgccg gccacccctg gcgacgagca
     2221 gttcctgacc ggcctcggtg agatcgccga gggtctccag ggtctcgtcg tcgatcaccg
     2281 aggttcctac cggaatccgc accagcctgg cgtcaccatg agccccggtc ttgttgcgcc
     2341 cgctgcccgg ctgtccgtta ccagctcggt acagcggctg gtagcggaaa tcgaccaggg
     2401 tattgagcga gtcgtcggca accagaatga cgtcgccgcc atctccgccg tcaccgccgt
     2461 cgggccctcc gcgggccacg tacttctccc tgcggaagct caggcagccc ccgccgccct
     2521 ttcctgcttc caccctgatc gtggcttcgt cgatgaactg catggcgtgc tcaacgcaaa
     2581 aaaaaccccg cgtccgcggg gtctggctgg ttcaggcttc cgccgtcgcc ggctccacgc
     2641 agacgaattt gcgcttgtgc ggaccccggg tgagaaattg aacgtagccg tccgcggttg
     2701 caaagagcgt gtgatcgcgc ccgcagccca cgttgtcgcc cgggtgaaac ttggtgcccc
     2761 gctgccgaac gatgatgttt cccgccgcaa cgaactggcc gccatacttc ttgacgccaa
     2821 gccgtttcga ctcggaatcg cgaccgttcc gggtgctgcc gccagctttc ttgtgagcca
     2881 tttcctgcct cccgccgttt agccgtttat gcccgtgatc ttgagctcgg tgtaccactg
     2941 ccggtggccc tgacgacgca tgtagtcctt gcgccgcttg aacttgatca cccttatctt
     3001 cttgccgcgg ccgctggaca caacctcggc ggtgacttta cctccgtcga cgaacggttg
     3061 gcccacggac acatcgtcac cgtcggcgac catgaggact ttgtcgaaca ccacctcgtc
     3121 gcccggttca cctgccagcc gctccactcg tatgacctct ccttcggtga cccggtgctg
     3181 cttgccaccg ctggagaaaa ccgcaaacat acctaaactc ctctatccag gcggcagcac
     3241 gatgccgcga cgaacaccca cccgagggct ataattcgag ccttgctgaa gcgattgtcc
     3301 cgggcttaag ctggcggccc gtcgcgtaaa atgctccggg acggcgccga aaggcgcgta
     3361 attctacggg caaggatatt gaattacaac gagttagccg atgccaggtg acccggccat
     3421 agcaaccaca acgacgcgaa tcagcgccga cccgaacgac gcgctgacgc ccgtgtacga
     3481 cctcgcgagg gacgatttcg cggaggtcaa tcgcctcatt ccccaccagc tgacttccga
     3541 cgtggagctc gtcgaggaga tcggccgcta catagtggac agcggaggca aacgcctccg
     3601 tccgctgatg gtgctgctgg gcgcccgctg ctgcggctac cagggcgatg accacgtgcg
     3661 ccttgccgcc atcatcgagt tcctccacac cgcgaccctt ctccacgacg acgtggtgga
     3721 ccgctccgag ctgcgccggg gccgggcaac ggccaacgcg ctctggggca atgccccgag
     3781 cgtgctcgtc ggcgattttc tctactcccg ggcttttcag ctgatggtcg agctggaaga
     3841 catggacatc atggcgatcc tagcggacgc gaccaacacc atcgcagcgg gcgaggtgca
     3901 gcagttcgcg agcatcggca acttcgcaat cgacgaagcc cagtacatgg agatcatccg
     3961 ctgcaagacg gcgctgctgt ttcaggccgc cacccatacg gccggcgtgc tgagcagcga
     4021 agatcccgac gaagtcgccg ctctcaaaaa tttcggcctc catttcgggc tcgcctacca
     4081 gctggtcgac gactggctcg attacgaggg cgactcctcc accctgggca agaacgtcgg
     4141 cgacgacctg gccgagggca agctgaccct gccgctgatt catgcgctgg caaacgcacc
     4201 ggacgccgac gccaatgtcg ttcgcgaagc cgtgagcgcc cgatccgccg agcgtctcgc
     4261 cgaagtgctt caggtcgtgc gttcctccgg cgcgttgagc tacacccgca gcgcggcaac
     4321 ccggcaatgc gcgcgggccg tggagtgcct aacgggactt cccgacaacg agtaccgcga
     4381 cgcgctggag acgctggcga acttctgcct ggatcgactc cgctagcggt gcgtcccgca
     4441 ccctcgattc tgctggcctg cctgtgtttc ttgcccttcg ccagcgcgct ggcggccgcg
     4501 cccgcgctcg agcaatgcga gctcagcggc agccatgggg tcggccgcgt ggaggccgtg
     4561 tgcggccggc tgcctcgacc cgagaacccc gaacagcccg acggagctcg gctcgacctt
     4621 ttcatcgcga agataccctc cctgagcccg gaacccgcac cggatgcgtt caccatcatc
     4681 aacggcggtc ccggggcttc ctcggtgtcg ctgttcgtcg acctcgccca ggcgttctcg
     4741 ggcgttctgc gcgagcggga catcgttctg gtcgaccaaa gaggcaccgg gcggtccaac
     4801 gccctcgaat gcccggagct ggagaaggcc gccgaggact actccatcga ggccgtccag
     4861 gaagcaacac gggcatgcct tgagtccctc gccggcgacc cccgtttcta taccacctcc
     4921 caggccgtgc aggatctcga ggcggtgcgg gcagcgctgg gatacgaaag gctcaacgtc
     4981 tatggcgtgt cctacggaac ccgggtttcc atgcactatc tgcgtcgtta tccggaccgg
     5041 gtcagaacgt tgatcct
//
LOCUS       DBNHOX010000678         3507 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_955214, whole genome shotgun sequence.
ACCESSION   DBNHOX010000678 DBNHOX010000000
VERSION     DBNHOX010000678.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_955214"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..321
                     /locus_tag="AC16UZ_14875"
     CDS             19..321
                     /locus_tag="AC16UZ_14875"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR6248019.1"
                     /translation="MNNAFLVSLSTYDDDVPAFAAVFAQVSGDWRRFFDEVETMARMT
                     SERRADAMEALREQHIAERGDDDGADEVECEALFHHGLDGDTAGAEHDHVGRGGHG"
     gene            complement(205..1563)
                     /locus_tag="AC16UZ_14880"
                     /pseudo
     CDS             complement(205..1563)
                     /locus_tag="AC16UZ_14880"
                     /inference="COORDINATES: protein motif:HMM:NF013132.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
     gene            1588..3009
                     /gene="sbcB"
                     /locus_tag="AC16UZ_14885"
     CDS             1588..3009
                     /gene="sbcB"
                     /locus_tag="AC16UZ_14885"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HIR6248020.1"
                     /translation="MAASYYWYDLETSGIEPRWDRVIQFAGLRTDLSLNPVGDEYCTY
                     VELPDDVLPSPEATLVTGITPDVTRREGVSEWQAMTRINELFSAPQTCVAGYNSLRFD
                     DEFIRFGFYRMLIDPYAREWQNGNSRWDIIDLVRATGALRRDGIRWPTDDEGLPVYRL
                     ELLTKANDLEHGQAHDAMSDVRATVALARLIRDCQPKLFDYYFNGRSKKQVKQLLEPF
                     GARVCLHVSGMYPRSRYCAAPVVSLCRHPKNSNSIIVADLSEDVEPLIGWTDEQIRAE
                     LFKEGSTSRPPLKEIRINRCPFVAGIEVMTGENWERIGFDRREIKERQRRLARPGLAQ
                     KIMRVYGDRPPPAAPDVEAALYDAFLQDADRGRCASLQEALGQGKWLDLDYSDRRLVS
                     LSTRLKSRSFAESLSEEERQDWHEFVRDKLNAEDAPWLTLKEFDARLDRIRQDSAGED
                     ARRERLLGKLEAYAGGLKARYGG"
     gene            complement(3089..>3507)
                     /locus_tag="AC16UZ_14890"
     CDS             complement(3089..>3507)
                     /locus_tag="AC16UZ_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248021.1"
                     /translation="DVSQWVEALMRTERLVEYYLVDDPPGLLMLGSDGAMFRAMILDP
                     VELSHQLEIARRYGAPDHIVKGIAERRLAVLLAGDSPADYYGAESFPWEENVCAVSPV
                     GDDGKWFVGVVRDAPVDIDFDPRLSSYDAYLATLER"
BASE COUNT          644 a         1124 c         1184 g          555 t
ORIGIN      
        1 gacgaggttc tggccgggct gaacaacgcg tttctcgtct ccctgtcgac ttacgacgac
       61 gacgttccgg ccttcgccgc cgtcttcgcc caggtgtccg gagattggcg acgcttcttc
      121 gacgaggtcg agacgatggc ccgcatgacc tccgagcgca gggccgatgc gatggaagcc
      181 ttacgtgagc agcacatagc agagcgtggc gatgacgatg gcgccgatga ggttgagtgt
      241 gaagccctct ttcaccatgg tctcgatggc gacacggccg gtgctgaaca tgatcacgtt
      301 gggcgcggtg gccacgggta gcatgaaggc gcaggatgcg ctcatcgcgg caggaatcat
      361 gaagagctcg ggcgatatgc ccgcgcccac ggcgcctgcg gcgagaatcg gcatcagcag
      421 ggtcgtcgtc gccgtgttgc tcgtggtttc ggtgaggaag gtaacggtga ggcagatggt
      481 gagcatgatc agcagcacat gcagcgttgc caggccggac agcagcgcgc cgatcgtctg
      541 gctgagaccg gaggcggtga acgccgcggc gattgtcagc cccgcgccga acagaatcag
      601 catgccccag gggatctgag tagcggtctc ccagtcgagc aaccgctcgc ccttcccgtc
      661 aggcacgacg aacatgatca ccacggcgag cagggcgacc atggcgtcgt tggtctgggc
      721 cagccccagc agtgccgtcc agcctccgaa cggctcgctg cgggttatcc aggcgacggc
      781 agtcagggcg aagaccatca acacccgaac ctcaccggcc tgccacgcgc ccacctcggg
      841 gacgtccacg gtgccctggt gtcccagtcg acgggtcagc cacacggcgg cgatgggcag
      901 gagcaccagc accacgggca tcgcaaacga catccagtcc gcaaacccga tctgacccac
      961 gccgagatcg ttgtacacgc ccatgaacac gacgttgggc ggggttccga tgggcgtgcc
     1021 gatgccgccg atgctggcgg cgtaagcgat gcccagcagg agcggcgtgg caagcttcgg
     1081 atcctcggcc ttttccagca ccgcgagcgc cacgggcaga agcatcagcg tggtcgcggt
     1141 gttggagatc cacatgctca gcaccgcgct ggcggccatg aacccgaaaa ccagtcgccg
     1201 gctgctgctg ccgccaaaag cgcggaccat ggtcagcgcg atgcgccggt gggccccgtt
     1261 gcgggccagg gctgtggaga ggatgaaccc gccgagcatg agcaggatga gcggatgacc
     1321 gtaagcctgg gcgacctgct gcggcgtcag cacccccagc aggggcagca tggccagggg
     1381 aagcaccgac gttgccggga tggggatagg ctcgaacacc caccacacca cgcagatcaa
     1441 cgttatgccc aaggttatgg ccgccgccgg ggtcgtcccg gtagagtaca tgaggtatcc
     1501 gacgaacaat gcgagaatgg ggccagcggg gagcgcgatg cgctttgcgt tggaagccga
     1561 catgggaagg caaggttata ggaagcgatg gcagccagct actattggta cgaccttgaa
     1621 acctcgggta tagagccgcg ctgggatcgc gtgatccagt tcgcagggct gcgcaccgat
     1681 ctgtcgctca acccggtcgg ggacgagtac tgcacctacg tggagctccc cgacgacgtg
     1741 ctgccgagtc ccgaggcgac gctggtgacg ggcattaccc ctgatgtcac ccgccgggaa
     1801 ggcgtcagcg agtggcaggc gatgacgcgc atcaacgagc ttttctcggc gccccagacc
     1861 tgcgtggccg gctacaacag ccttcgcttc gatgacgagt tcattcgctt cggcttctac
     1921 cggatgctca tcgacccgta cgcccgggag tggcagaacg gcaactcgcg ctgggacatc
     1981 atcgatctcg tccgggcgac gggcgcgctg cggcgcgacg ggatccgctg gcccaccgac
     2041 gacgaaggtc ttcccgtcta ccggctcgag ctgctcacca aggccaatga cctcgagcac
     2101 ggccaggccc acgatgccat gtcggacgtg cgcgctacgg ttgcgcttgc gcggttgatt
     2161 cgcgactgcc agccgaagct gttcgactac tatttcaacg gccggtcgaa gaagcaggtc
     2221 aagcagctgt tggaaccgtt cggagcgcgc gtctgcctgc atgtctcggg catgtatccg
     2281 cgcagccgct actgcgccgc gccggtggtg tcgctgtgcc gccaccccaa gaacagcaac
     2341 tccatcatcg tcgccgacct cagtgaagac gtggagccgc tgatcggctg gaccgacgag
     2401 cagatacgtg cggagctgtt caaggagggt tccaccagtc gccccccgct gaaggagatc
     2461 cgcatcaatc gctgcccgtt cgtggcgggc atcgaggtca tgaccggcga gaactgggag
     2521 cgcatcgggt tcgaccgtcg agagatcaag gaacgccagc ggcgtctcgc ccggcccgga
     2581 ctggctcaga agatcatgcg cgtatacggc gatcgccccc caccggcagc acccgatgtg
     2641 gaagcggcgc tttacgacgc ctttctccag gatgcggatc gcggtcgctg cgccagcctt
     2701 caggaggcgc ttggccaggg gaagtggttg gacctcgact attcggaccg ccggctggtt
     2761 tcgctctcga cccggctgaa gtcccgcagc tttgcggagt cgctctccga ggaggagcgg
     2821 caggactggc acgagttcgt gcgcgacaag ctcaatgctg aggatgcgcc gtggctcacg
     2881 ctgaaggagt tcgacgcgcg gctggatcgg atccggcagg actcggccgg agaggacgcc
     2941 cgacgcgaga ggctgctcgg gaagctcgaa gcctacgccg gggggctgaa agcacgctac
     3001 ggcggctgac acggtccaag gccgtcccgg cggcccggac cgaggtgcca gccggtcggg
     3061 tagcgccggc cccagtcgcg atgccgcttc agcgctccag ggttgccagg taggcgtcgt
     3121 aggaggaaag cctcgggtcg aaatcgatgt ctaccggcgc gtctcgcacc acgccgacaa
     3181 accacttccc gtcatcgccc acggggctga ccgcgcagac gttctcttcc cacggaaagc
     3241 tctcggcgcc gtaatagtcg gccggggagt cgccggcgag cagcacggcc agccggcgct
     3301 ccgcgatgcc cttcacgatg tggtcgggcg cgccataccg gcgcgcgatc tcgagctgat
     3361 ggctcagttc aacggggtcc aggatcatcg ctctgaacat cgcgccgtcg ctgccgagca
     3421 tcagcaggcc cggcgggtca tccaccaggt agtactcgac cagccgctcg gtgcgcatga
     3481 gtgcctcgac ccattgcgag acgtcgg
//
LOCUS       DBNHOX010000679         1871 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1211578, whole genome shotgun sequence.
ACCESSION   DBNHOX010000679 DBNHOX010000000
VERSION     DBNHOX010000679.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1211578"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="AC16UZ_14895"
     CDS             <1..215
                     /locus_tag="AC16UZ_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248022.1"
                     /translation="GRPLSDLIKEYLEKDFLGTLRAIQGFIPKEQLIGPMEESEGSFS
                     VQVNFVEPEKVVEEGAASEGRETRPH"
     gene            complement(339..425)
                     /locus_tag="AC16UZ_14900"
     tRNA            complement(339..425)
                     /locus_tag="AC16UZ_14900"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(389..391),aa:Leu,seq:caa)
     gene            547..>1871
                     /locus_tag="AC16UZ_14905"
     CDS             547..>1871
                     /locus_tag="AC16UZ_14905"
                     /inference="COORDINATES: protein motif:HMM:NF036848.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HIR6248023.1"
                     /translation="MSNTPILVGVAQVEQRIEDVREGEEPLAMMIDATRLAAQDAGCP
                     GLLSEANSVRVIRGMWPYANPARAVGEAIGNPGAETALTPFGGNSVQSTLNHSAIDIR
                     DGVHDVIIITGAECGRSQAKAGRAGVDLNWSEVPGTPDRYIGEDMEMRCPAERGLGMG
                     RPIQMYPMMENHLRYRRGDTIEEQLIRISELWASFSAVAAENPHAWMRKAKTAEQLRT
                     ISVENRPVSFPYPKLMNSNNNVDQGAALILCSTEKAKALGIPEEKWVYPWAGTDAHDT
                     YFVSNRDSMSRSPAIRLAGRRCLELTGLTPLDLAFVDVYSCFPVAVQVAAEEIGLDTT
                     RPLTITGGLTFAGGPLNNYVMHSIATMVEKLRGARGEKGLITANGGYLTKHAFGVYGT
                     EPPATPFQHEDLQPAVDATATRKVVTEWTGEATVESYTVMYKGDELAVG"
BASE COUNT          388 a          579 c          590 g          314 t
ORIGIN      
        1 agggcaggcc gctgtcagat ctgatcaagg agtacctgga gaaggacttt ctgggaacct
       61 tgagagccat ccaggggttc atacccaagg agcagctgat cgggccgatg gaagagtcag
      121 agggttcgtt cagtgttcag gtgaacttcg tggagcctga gaaggtcgtc gaggaggggg
      181 ccgcttcaga gggcagggag acgcgaccgc actgagtgaa cgatagcttt cagctcatcg
      241 aaaacctcaa aaaccgcccg aaatgttgca ccggtgttgc accagcactg acgttctaat
      301 gcaacatttc gggaccggcc tgaaacccgc atttttactg gtgcctgggg tgggaatcga
      361 acccacactt ggttgcccaa accagatttt gagtctggcg cgtctaccag tttcgccacc
      421 caggcagcga tcgcggattg ttgcacaggc cgagaaagcg cggcaatggc acctttgcga
      481 cagcgcagct tcctttcggt atcatccccg ccccttaaaa gattcagtca ggatttcttc
      541 gagaacatga gcaatacccc cattctggtt ggcgtggccc aggtcgagca gcggatcgag
      601 gacgtgcgcg aaggcgagga gccgctggcg atgatgatcg acgccacgcg actggcggcg
      661 caggacgctg gctgccccgg cctgctcagc gaggcgaact ccgtgcgggt gatcagaggc
      721 atgtggcctt acgccaatcc ggcaagggcc gtgggtgaag ccatcggcaa tccgggcgcg
      781 gagaccgcgc tcaccccgtt cggcggcaac agcgtgcagt cgacgctgaa ccatagcgcg
      841 atcgacattc gcgatggcgt ccacgacgtc atcatcatca ccggagccga gtgcggccgc
      901 agtcaggcta aggcggggcg agcgggcgtc gacctgaact ggagcgaagt tcccggcacg
      961 cccgaccggt acattggcga ggacatggaa atgcgctgcc ccgccgagcg cggtctcggc
     1021 atgggtcgcc ccatacagat gtacccgatg atggaaaacc atctgcgcta tcgccggggc
     1081 gataccatcg aggaacagtt gatccgtatc tccgagctgt gggcgtcgtt cagcgccgtc
     1141 gccgcggaga atccccacgc gtggatgcgc aaggctaaaa ccgcggagca gcttcgaacg
     1201 atctccgtgg agaaccggcc cgtctccttt ccctacccca agctcatgaa ctccaacaac
     1261 aacgtggatc agggcgcggc cctcattctc tgctcgaccg agaaagcaaa agcgctcggc
     1321 atccccgagg agaagtgggt gtacccctgg gcgggcaccg acgcccacga tacctacttc
     1381 gtgtcgaacc gggacagcat gagccgctcg cccgccattc ggctcgccgg ccgacgctgc
     1441 ctcgagctga ccggactgac gccattggat ctggccttcg ttgacgtcta cagctgcttc
     1501 ccggtggccg tgcaggtcgc cgccgaagag atcggcctgg acacgacacg gccgctcacc
     1561 atcaccgggg gtctgacctt cgccggaggg cccctgaaca actacgtcat gcactccatc
     1621 gccacgatgg tggagaaact gcgcggcgcc cggggtgaga aaggcctgat caccgccaac
     1681 ggtggctatc tcaccaagca tgctttcggc gtgtacggta ccgagccacc tgcaacgccg
     1741 ttccagcacg aggacctgca gccggccgtc gatgccacgg ccacccggaa agtcgtcacg
     1801 gaatggacgg gcgaggccac ggtggagagc tacacggtca tgtacaaggg tgacgagctc
     1861 gccgtcgggc a
//
LOCUS       DBNHOX010000680         1907 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_826277, whole genome shotgun sequence.
ACCESSION   DBNHOX010000680 DBNHOX010000000
VERSION     DBNHOX010000680.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_826277"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="AC16UZ_14910"
     CDS             complement(<1..153)
                     /locus_tag="AC16UZ_14910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6248024.1"
                     /translation="MNPVLSVAGLAKRYVDGSAERVVLAEVSLEVGPGEILAVCGPSG
                     SGKSTLL"
     gene            complement(164..919)
                     /locus_tag="AC16UZ_14915"
     CDS             complement(164..919)
                     /locus_tag="AC16UZ_14915"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6248025.1"
                     /translation="MQPVRKDNIRLAATILLVRDVGGGPEVFMVQRPGGVDFPDLHVF
                     PGGKVDAADFVPELVTDLDDRSASQRLGVEAGGLRYWVAAIRECFEECGVLLADRDGE
                     LFTPRTDAEAERFAGHRHALISGELDFAAMCRQEGLAIRAGRAHYFSHWITPEVAPRR
                     FDTRFFIAHMPTGQVTAGHAWETAAEAWVAPGRALELFETGRWQMIAPTLTTLQTIAR
                     YSGVAHMLADVAAEAHLDELTADLRHQGMYPLR"
     gene            1008..1304
                     /locus_tag="AC16UZ_14920"
     CDS             1008..1304
                     /locus_tag="AC16UZ_14920"
                     /inference="COORDINATES: protein motif:HMM:NF015807.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HIR6248026.1"
                     /translation="MKEKYYKVLVRLPPEMREMLAESARHYRRSRNSDIVARLQESFR
                     GIADSARGRGLAPPMHDQFEQLFRADLSPQEQSLVTAFRRLSSDQQTALLALLN"
     gene            1328..>1907
                     /locus_tag="AC16UZ_14925"
     CDS             1328..>1907
                     /locus_tag="AC16UZ_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6248027.1"
                     /translation="MSLSPYHHPTTICLVDDNQSFLDSMILEMPSMWACRAFTDPHAA
                     AEFLNQPIGLPPLAERCLSRETSTGSDAVIHIDLGLIEQEVNHPDRFRRISVLVADYA
                     MPSMNGLELCAAARDPHLRKAMLTGVADEKLAVEAFNEGLIHRFIPKHGTSATAIARE
                     YIAELQAEYFEQYTALLQGSLTVGKPGFLTDPD"
BASE COUNT          363 a          659 c          575 g          310 t
ORIGIN      
        1 cagcagagtg gacttgccgg agccgctggg cccgcaaacg gccagaatct ctcccggacc
       61 cacctctaag gagacctccg cgagcacaac ccgctcggcg cttccgtcaa cgtagcgttt
      121 ggcaaggccg gcaacggaca ggaccgggtt catgattctg ccctcatcgc agtggataca
      181 tcccctgatg gcgcagatcg gccgtcagct cgtcgaggtg cgcctccgcg gccacatcgg
      241 ccagcatgtg cgcaacgcct gaataacgcg cgatggtctg cagggtcgtc agcgtgggcg
      301 cgatcatctg ccatcgcccc gtctcgaaaa gctccagggc cctgcctggc gctacccagg
      361 cctcggccgc cgtttcccaa gcgtgcccag cggtgacctg gcccgttggc atatgggcaa
      421 tgaagaaccg cgtgtcgaag cgccgcggcg ccacctcagg cgtgatccag tgactgaagt
      481 agtgcgcgcg gccggcccgg atggcgagcc cttcctgccg gcacatggcc gcgaaatcca
      541 gctcgccgga gatgagcgcg tgacggtgtc cggcaaagcg ctccgcttcg gcatccgttc
      601 gcggggtgaa gagctcaccg tcgcgatccg ccagaagcac gccgcactcc tcgaagcact
      661 cgcgaatggc cgccacccag tatcgcaggc cgcccgcctc cacgcccaac cgctgcgacg
      721 ccgaccggtc gtccaggtcg gtgacaagct cgggcacgaa gtcggccgcg tccaccttgc
      781 cccccggaaa gacgtgcagg tccgggaagt cgacgccgcc gggacgctga accatgaaca
      841 cctcggggcc gccgccaacg tcgcggacga gcaggatggt cgcggcgagc ctgatgttgt
      901 cttttctaac gggctgcatt tgcaggaact gatatcaaat tgccatctct tgtcgggctc
      961 ggcatgccgc gctagtctga gaccgaccac gcggccgaga cctcaaaatg aaagaaaaat
     1021 attacaaggt cctcgtgcgt cttccgccgg aaatgcgaga gatgctcgca gagtcggcgc
     1081 gacactaccg gcgcagcagg aactccgaca ttgtagcgcg tctgcaggag tccttcagag
     1141 gcatagcaga ttccgcccgg ggacggggac ttgcgccacc catgcacgac cagttcgagc
     1201 agctgttcag ggccgacctc agcccgcagg aacagagcct cgtcacggcc tttcgccggc
     1261 tgagcagtga ccagcagacg gcgctgctcg ccctgctcaa ctgacgctgc ggaccgggac
     1321 acccctgatg tcactttcac cctatcacca ccccaccacg atctgcctgg tggacgacaa
     1381 tcaatccttt ctggacagca tgatcctgga gatgcccagc atgtgggctt gccgggcgtt
     1441 cacggacccc cacgcagctg cagagttcct gaaccaaccc atcgggctgc cgccgctggc
     1501 tgagcgctgc ctttcccggg agacctcaac gggctccgac gcggtcattc acatcgacct
     1561 cggcctcatc gagcaggagg tcaatcatcc ggaccggttc aggcgcatat cggtgctggt
     1621 ggccgattac gccatgccat ccatgaacgg gctggagctt tgcgccgcgg ccagggatcc
     1681 ccacctgcgc aaggccatgc tcaccggcgt cgcggatgaa aagctggcag tcgaggcctt
     1741 caacgagggt ctcatccacc ggttcattcc caaacacggg acgtcggcga ccgccatcgc
     1801 ccgggaatac atcgccgagc tgcaggccga atatttcgaa cagtacacgg ccctgcttca
     1861 gggcagcctg accgtgggca agcccggctt tctgaccgat cccgacg
//
LOCUS       DBNHOX010000681         1707 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_569531, whole genome shotgun sequence.
ACCESSION   DBNHOX010000681 DBNHOX010000000
VERSION     DBNHOX010000681.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_569531"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /gene="tolA"
                     /locus_tag="AC16UZ_14930"
     CDS             <1..464
                     /gene="tolA"
                     /locus_tag="AC16UZ_14930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HIR6248028.1"
                     /translation="ADPPQAPPPDVGAERRRAAQAERQERLEQLAEASFLDALETEAA
                     DLAESLAAEEAEAVAQTFRYGIYERVVANWSRPPSARNNMQARLLVELVPTGDVVSVT
                     LIEGSGNEAFDRSAEAAVRKARRFEVPKDSDVFERYFRRFTLLFRPEDLLR"
     gene            461..>1707
                     /gene="tolB"
                     /locus_tag="AC16UZ_14935"
     CDS             461..>1707
                     /gene="tolB"
                     /locus_tag="AC16UZ_14935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HIR6248029.1"
                     /translation="MINFARCLSALVCLVLMLTPAATLAAESLDTIIIDAGYDKQTRI
                     AVVPFRQGPEFSDRQQMGDIIGFDLVRSGQFSPLGMENMLSFPTTREEVFFRDWRVLG
                     IEYLVIGRTGVDAAGNLEVTYELFDVFNERRMTGGTTTALRSQWRDVAHRVADEVFEA
                     ITGIPGAFSTRILYVLAQNVGSPDEAFRLEVADSDGERSRTLFSSTQPLLSASWSPDG
                     TRVAYVSFEGGRPSIVLQNVATGARERLTQFSGINGSPVFSPDGKRLALVLSRDGNPE
                     IYIMDLDDRRLRRITTHHAIDTEPSWTPDGKGVIFTSDRGGRPQIYQLDLATNFIERL
                     TFQGDYNARARLLPDGKHLIFVHRRQGVFHIAWQDLERDDVRVLTQTSLDESPSLAPN
                     GTMLIYATQDRGRGILAVVSIDGR"
BASE COUNT          281 a          572 c          549 g          305 t
ORIGIN      
        1 ctgcggaccc cccccaggcg cctccgccag acgtcggggc cgagcgcaga cgggccgcac
       61 aggccgagcg gcaggagcgt ctcgagcagc tcgccgaagc ctcgtttctg gacgccctgg
      121 agaccgaggc cgccgatctc gccgagagcc tggctgcgga ggaggccgaa gccgtcgcgc
      181 agaccttccg ttacgggatc tacgaacgcg tggtcgccaa ctggtcgcgg ccacccagcg
      241 cgcgaaacaa catgcaagcc cgtttgctcg tcgagctcgt gccgactggc gacgtcgttt
      301 cggtaacgct catcgagggg agcggcaacg aggcattcga ccgctcggcc gaggcggccg
      361 tgcgaaaagc acggcgtttc gaggtgccga aagattcgga cgtcttcgaa cgctacttcc
      421 gtcgtttcac actgcttttc cgtccggagg acctgctgcg ttgattaact ttgcccgctg
      481 cctgtccgcg ctcgtctgcc tggtcttgat gctgacgccg gccgcgaccc ttgccgcgga
      541 gagcctggac accatcatca tcgacgcggg gtacgacaag cagacgcgca tcgccgtggt
      601 gccgttccgg caggggccgg agttttccga tcgtcagcag atgggtgaca tcatcgggtt
      661 cgaccttgtt cgcagcggcc agttttcgcc ccttggcatg gagaacatgc tgtcgtttcc
      721 caccaccagg gaagaagtct tcttccgcga ctggcgcgtg ctcggcatcg agtatcttgt
      781 catagggcgc acgggcgtgg acgccgccgg caatctcgag gtcacctacg agctgttcga
      841 cgtgttcaac gagcgtcgga tgacgggcgg cacgaccacg gcgctgcgca gccagtggcg
      901 ggacgtggcc catcgcgtcg ccgacgaggt cttcgaggcg atcaccggca ttcccggcgc
      961 gttctccacc cgcattctgt acgttctcgc gcagaacgtc gggtccccgg acgaggcgtt
     1021 tcggctggag gtggccgact cggacggcga gcggtcgcgg acgctgttct cgtccaccca
     1081 accactcctt tcggccagct ggtcgcccga tgggacccgc gtggcctacg tatccttcga
     1141 gggaggacgg ccgtccatcg tgctgcagaa cgtcgccacg ggggcgcggg agcggctgac
     1201 ccagttttcg ggaatcaacg gttcccccgt cttctcgccg gatggcaagc ggctcgctct
     1261 ggtgctctcg cgggacggca accccgagat ctatatcatg gatctcgacg atcggcgcct
     1321 gcgccgcatc accacccacc acgccatcga caccgagccc agttggacgc cggatggcaa
     1381 gggcgtgata ttcacctcgg accgcggcgg caggccacag atctaccagc tcgacctcgc
     1441 gaccaacttc atagagcggc tcacgtttca gggtgactac aatgcccggg cgcgcctcct
     1501 gccggacggc aagcacctta ttttcgtaca ccgtcgacag ggggtgtttc acatagcctg
     1561 gcaggacctc gagcgcgacg acgtcagggt gctcacccag acgtccctgg acgagtctcc
     1621 gtcgcttgcg cccaacggca cgatgctaat ctatgcgacg caggatcggg ggcggggtat
     1681 actcgccgtc gtttcgatcg acggccg
//
LOCUS       DBNHOX010000682         1554 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_185037, whole genome shotgun sequence.
ACCESSION   DBNHOX010000682 DBNHOX010000000
VERSION     DBNHOX010000682.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_185037"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1319)
                     /locus_tag="AC16UZ_14940"
     CDS             complement(162..1319)
                     /locus_tag="AC16UZ_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR6248030.1"
                     /translation="MDWLRTPVDRLQEFKPPFCPRQDCSQHTRSAGFRFKKDGHYRLS
                     RGTLVQRFRCKTCGRRFCRRAFSLAYYRKRPELLRPVAAGLQAGSAHRQIARSLGCAP
                     STVTRISARLGRHAMLLLWRALAALDGQLDEPIVLDHFETFELSQDLPLGVATPVGAR
                     SWFVYGVDPAPHARTGRRSTVQEARRKKRARRTTRGGYIGSTRRVLNRLLPLCPADRR
                     LRLISDGHPSYAKAVERHPRVEKIELECHPNPPRGPKGSPRSARAMARDRAMFPVDLL
                     HGLLRHSLAAHKRETIAFGRRLNAIVERLFLTAVWRNFVKGVSERRGDPTTPAMRVGL
                     TDRPWPWERVLGRRLFPDRERLDGTWLELYRRDWVTPELRSNTRHRKVFAY"
BASE COUNT          264 a          505 c          526 g          259 t
ORIGIN      
        1 tgcatcatcc acgcctcgtt cggattcggc gtgatcccgg cgatctcgac gcgacgtgtg
       61 gccagtccaa tgacgaagaa cacatagaag gtgaccatgg tggtgcgcag attgcggctc
      121 gagcggcgtt ccgatgaagg agatcttgcc gatgcaggtt ttcagtaagc gaacaccttc
      181 cggtgccgcg tgttcgaccg cagttccggc gtgacccagt ccctccggta aagctcgagc
      241 cacgtgccat cgagcctctc gcggtccgga aacagacgtc gccccagcac ccgctcccac
      301 ggccacggcc ggtcggtcaa accaacgcgc atcgccgggg tcgtcggatc gccccggcgc
      361 tcggaaaccc ctttgacgaa gttcctccac accgcggtca ggaacaaccg ctccacgatc
      421 gcgttgagcc gccgcccgaa ggcgatcgtc tcccgcttgt gcgcggccag cgagtggcgt
      481 agcagcccgt gaagcaggtc gacggggaac atcgcacggt cgcgcgccat ggcccgggcc
      541 gaacgaggtg atcccttggg cccgcgcggc gggttggggt ggcactcgag ctcgatcttc
      601 tcgacgcgcg ggtgccgctc gacggccttg gcgtaggacg ggtgtccgtc gctgatcaga
      661 cgaaggcgcc ggtccgcggg acacagcggt aggagcctgt tcaacacacg ccgcgtcgag
      721 ccgatgtaac ccccacgggt cgttcgccgg gccctcttct tccgacgcgc ctcttgaacg
      781 gtggaccggc gccctgtccg cgcatgaggt gcgggatcga ctccgtaaac gaaccaggaa
      841 cgggctccca cgggtgtggc cacgccgagc ggtaggtcct ggctgagctc gaaggtttcg
      901 aagtggtcca agacgatggg ttcgtccagc tgaccgtcca gtgcggccag ggcacgccag
      961 aggagcagca tggcgtgtcg gccgagacgg gcactgatgc gggtgacggt cgagggggca
     1021 cagccgaggc tgcgagcgat ctgacggtgg gccgagccgg cctgcagccc ggcggcgacg
     1081 ggccgcagca gctcggggcg cttgcggtag taggccaggg aaaacgcccg tcggcagaag
     1141 cgccggccgc aggtcttaca tcgaaaccgc tggacgagtg tgccgcggga caggcggtag
     1201 tgcccgtcct tcttgaagcg gaacccggct gatcgtgtgt gttgcgagca gtcctgccga
     1261 gggcaaaacg gcggcttgaa ctcttgcagt cgatcgaccg gtgtgcggag ccagtccatg
     1321 gcccgctcca aaccaaggat cgtgccacac cggggactgc ggcaaacgcc tgcagatcaa
     1381 cgacttgaga attcgccccg tgtccgtgtt gtccgtcggc ggacgccccc ctgtccgcat
     1441 gcggccgccg cagtctttgc accaccatga ttcctttttc atcggggagg acgattttca
     1501 tgcccagcgt gcggctggtt tcgggcaccg agaacgagct tcgcttttcc cact
//
LOCUS       DBNHOX010000683         3334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_441069, whole genome shotgun sequence.
ACCESSION   DBNHOX010000683 DBNHOX010000000
VERSION     DBNHOX010000683.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_441069"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="AC16UZ_14945"
     CDS             <1..429
                     /locus_tag="AC16UZ_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248031.1"
                     /translation="GRSHSVLFAALLLVVQLPLGVLSGGAMAHDDAQHCGDCPDGSPS
                     SPMQGTDSHGDVAAGHQGDSGCGFDCSPDGSTDTGHAGCGFDCAMLSSGHCGSPVSPT
                     LAAMALLATTSLADAFGRDRSSVMLPDPPLFDFLRPPTRS"
     gene            503..1015
                     /locus_tag="AC16UZ_14950"
     CDS             503..1015
                     /locus_tag="AC16UZ_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248032.1"
                     /translation="MNTLKIIALVPAAFVAVAAAPYAGAAAKPTAGHDHAAMVQDHQA
                     KSATSTNTVAAHEAMANHCEGMMDHAEAAATGHSDTAGRHPCRAEAQSATLTFEQHLA
                     AARELEAKAAAASAKASEHEKMARAGGAPKDDGRSMDNHCSELVIRYRAQAESYSAQA
                     AEHKRLAAAR"
     gene            1250..2299
                     /locus_tag="AC16UZ_14955"
     CDS             1250..2299
                     /locus_tag="AC16UZ_14955"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR6248033.1"
                     /translation="MVAHSIAIALGGGGARGLAHLGVLEVLDDQGIRPAFLSGTSMGG
                     LIAALRATGTDPDDITATARRFKLPSRFLPGRLLVWDTIFASAVPRLEGRTFEDLATP
                     LVVSAVDLMTGEEVALCSGSLLPAVRATCAVPGVFPPEKVGARCLVDGGVTNMLPVDL
                     AWACDPDIVVAVNIVASPPSTLRLDSAYCRAATRLGKVVANPLTALFSYEVAMRAAEI
                     ALDRQRAMAVAMTGPEVLIDVDLSHVAIDDFQRFDEIVEAGRNATRRALPGIQAALSA
                     PADGGERATADATLHIDPVCRMTVSSARARATGEREGKTFYFCSTSCRDIFARHGDRY
                     VGATALRPPIVRGKS"
     gene            complement(2713..>3334)
                     /locus_tag="AC16UZ_14960"
     CDS             complement(2713..>3334)
                     /locus_tag="AC16UZ_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248034.1"
                     /translation="CTFAVGKWPPVVLEPPNHGLPRCAGPGPGRRLGASRKKWIRMGL
                     AEALRAFTAVVAALPLTACLSILGGEAVSKDAKALPDNERAVLLGRSQWYLAWGRDIS
                     LFKIDGVLYDVDGLKQAEVAPGHHAVVIEQIAFFGGMGGNRQRYYFTLELQAGHHYQI
                     LSVQNWHQWPFLDQHQEQCRVEIEDVPPDLDQGTNLQLPCEVYNGN"
BASE COUNT          565 a         1107 c         1082 g          580 t
ORIGIN      
        1 ggtcggtctc attccgtcct gttcgcagcg ctgctcctcg tggtacagct gccgctcgga
       61 gtgttgtcag gcggagccat ggcgcacgac gacgcgcagc attgcggcga ctgccccgac
      121 ggcagtccga gcagcccgat gcagggaacg gactcccatg gtgacgtggc agcaggtcac
      181 caaggtgatt cgggctgcgg cttcgactgt tcgcccgatg gctcgaccga taccggtcat
      241 gcgggctgcg ggttcgactg cgccatgttg agcagcggtc actgcggctc tcccgtcagc
      301 ccaacactcg ccgccatggc gctgctggcc acgacgtctc tcgccgacgc attcggccgc
      361 gaccgttcgt cggtcatgct gccagaccca ccgctcttcg actttctccg tcctccgact
      421 cgcagctgaa tcagctgcac tcggtttccc atcgcctggc gaggcccagg cgacctgatg
      481 acttctttcg acggagattg aaatgaacac cctcaagatc attgcccttg tcccggccgc
      541 gttcgttgcc gttgccgcgg ctccctacgc cggcgccgca gcgaaaccca cggcaggcca
      601 tgaccatgct gcgatggtgc aggaccacca ggccaagtcc gccacgtcga caaatacggt
      661 ggctgcgcac gaggcgatgg cgaaccattg tgaaggcatg atggaccatg cagaggcggc
      721 ggctaccggg cattcggaca cggctggccg gcatccatgc cgcgcagagg cccagtcggc
      781 gacactcacg ttcgagcagc acctcgccgc cgctcgcgag ctcgaagcca aggcggcagc
      841 tgccagcgcc aaagcgtccg agcacgagaa gatggctcgc gctggtggcg cgccgaagga
      901 cgacgggcgg tcgatggaca atcactgcag tgagctggtg atccggtacc gtgcacaggc
      961 cgagtcgtac agcgcgcagg ccgcggagca caagcggctc gcagctgcca ggtagttgcg
     1021 tgacgggagg ctacgggcga ccgtagcctc ccccgctgtc ggcgggcaca tcgcgcgcgt
     1081 cgagcatccg gtcccggtcg tcagcgaact tctggatccg ggatgcgcca ggcctcgcgc
     1141 cgtcatcgtc gacgccagac gacgctccct gcgacgcaat tgccgtcggc accacctacg
     1201 tcacgaagtc cgtattgaaa gcgaggcgac ccagcgtagt ctgaccaaga tggttgcaca
     1261 ttccatcgcc atagcgcttg gcggcggcgg cgcccggggc ctcgcccacc tgggggtgct
     1321 cgaggtgctc gacgaccagg gtatccggcc ggcgttcctc tcaggtacga gcatgggcgg
     1381 gctgatcgcc gcgctgcgtg ccaccgggac cgacccggac gacatcacgg ccacggcccg
     1441 gaggttcaag ctgccaagcc gcttcctgcc ggggcggctg ctcgtctggg acacgatctt
     1501 cgcctccgca gtgccacggc tggaggggcg gacattcgag gacctggcga ccccgctcgt
     1561 ggtgtcggcg gtggacctga tgaccggcga agaggtggca ctctgcagcg ggtcactgct
     1621 ccctgcggtc agggccacct gcgcggttcc tggcgtgttt ccgcccgaga aggttggcgc
     1681 ccgctgcctc gtcgacggcg gcgtgaccaa catgctgccc gtggatctcg cctgggcgtg
     1741 cgaccctgac atcgtcgtcg ccgtgaatat cgtcgcttcg ccaccttcga ccctgcgtct
     1801 cgactcggct tactgccggg cggcaacgag gcttggcaag gtggtggcaa atccactcac
     1861 cgccctgttt tcctacgagg tcgcgatgcg tgccgccgag atcgccctgg accggcaacg
     1921 ggccatggca gtcgcgatga ccggaccgga ggtcctgatc gacgtcgacc tcagccacgt
     1981 agccatcgac gacttccagc ggttcgacga gattgtcgag gccgggcgga atgccacgcg
     2041 gcgggcgctt ccgggaatcc aggctgccct ttcggcgccg gccgatggag gcgagcgggc
     2101 caccgccgat gccacgctgc acatcgaccc ggtgtgccgc atgaccgtga gctcggcgcg
     2161 ggcacgggcc acgggtgagc gtgaaggcaa gacgttctac ttctgctcca ccagctgccg
     2221 cgacatattc gcgcgccacg gtgaccgata tgtcggggca acggccctgc gcccaccgat
     2281 cgtgcgcggc aagagctagg ccaccggagt gccggaagcc ggccgcgccc ggcctcgacc
     2341 tgctggcgcc catggagttc gggtgcgaac cgtcccgcga gatggcgttg ggtggggctg
     2401 aaaggacaag gggctcacgc ggtttcgtaa accctcggtc tggatggcgc gtgcggagag
     2461 gttactcgcg gcactgcgtg gagcctcgcc gaacatcctg aaccgaaggt tcgtaggcgg
     2521 tcctgagaga cagcagaact ttaatggcca acagcctgta gcgccttagg gtcctgaaac
     2581 cagcctgtcc ccggcacggg cagtctcaag gcgtcgcctc tgcggcgcat gtccgatgac
     2641 ggccagaaac ggacgatcgt ggactgtaga agtcccgccc tgcagcgttg tcgagaaatt
     2701 gcatccagcc gactagttgc cgttgtagac ctcgcaaggc agctgcaagt tcgttccctg
     2761 gtcgaggtct ggcggcacgt cttctatttc gactctacat tgctcttgat gttgatccag
     2821 aaacggccac tgatgccagt tctgtacaga caggatctgg tagtggtgtc cggcctggag
     2881 ctccagggtg aaatagtagc gttgcctatt gccgcccata cctccgaaaa atgcgatctg
     2941 ctcgatgact accgcatggt ggccgggtgc cacttcggcc tgtttgagtc cgtccacgtc
     3001 gtagaggaca ccatcaattt tgaacagcga gatgtcccgg ccccaagcga gataccactg
     3061 cgaccgcccg agcagcaccg ctcgttcatt gtcaggaagc gccttggcgt ctttcgatac
     3121 ggcctcccct cccaggattg acaggcatgc cgtcagcggc agcgctgcca cgactgcggt
     3181 gaacgcccga agcgcttcgg ccaagcccat gcgaatccac ttctttcgac ttgcaccgag
     3241 cctccgcccc ggtccgggac cagcgcatcg tggcaagccg tggtttggag gttcgagcac
     3301 caccgggggc catttgccga cagcgaaggt gcaa
//
LOCUS       DBNHOX010000684         1862 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_890429, whole genome shotgun sequence.
ACCESSION   DBNHOX010000684 DBNHOX010000000
VERSION     DBNHOX010000684.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_890429"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            457..1476
                     /locus_tag="AC16UZ_14965"
     CDS             457..1476
                     /locus_tag="AC16UZ_14965"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR6248035.1"
                     /translation="MLTIGLDVHERSTSICILDERGDRVKESRVAGHPREAIGRLSTL
                     DTPFQACFEASTGYGWMYDELSQVAERVVVAHPGKLRLIYRSRRKNDRNDAYRLARLM
                     LVDELPPVHVPPVDFRNWRSLIELRNRYVGQRTRIKNSLRALLRTCGVKGPRSLWSKK
                     GLAWLEALQLDEDRAFTRAMLLNDLAAQQQNIAETERRLKKIASRQPAVRLLMTIPGV
                     GIRTAEAFVAYTHDAKRFRGRSIGAYLGLVPRQDSSGETNRMGRITREGPSTVRKMLA
                     EATWQSIRRSQRVRRHFERIMGGRPDRRKKALVATSHYLARVMLAMLKNGSVWREDVA
                     SAVAA"
BASE COUNT          330 a          578 c          646 g          308 t
ORIGIN      
        1 cggcttgaca ccacgcgggt gcggcatctc ttattgacat tttattgtca tcaatgagcc
       61 cctgccgtga ccacctgctg cccgagctgc tcttccgctt ccgccctcgg aggcgaagtc
      121 gcgtccatct gcaccgagtg cgccgccgtc tccgtcgtcg gggcgccgtt cagcccgggg
      181 atgctgctcg cgacgacagc ggtcgccgtg accgtcgtcc tcgcctggaa ggtcgcccgg
      241 cgcttcgtgc agcgtacgcc tcgcctcgct ccgagcccgg cgtgccgaaa acgcccgctc
      301 gggaacgccg ccgctctcgc gggtcgccga aaacgctcgc tcaggaacgc cggatcgaat
      361 ccggcgcgtg cccccggagg agcgagtgag tctatgtagc aagcccctgg ggcgggtggt
      421 acaccacccg ctcttcaaac ccccaggaga cttgccatgt tgacgattgg tctggacgtg
      481 cacgagcggt cgacgtcgat ctgcatcctc gatgagagag gcgatcgggt caaggagtcg
      541 cgtgtcgcag ggcatccgag ggaggccatc gggcggctct cgacgctgga cacgccgttc
      601 caggcctgct tcgaggcctc gacaggctac ggctggatgt acgacgagtt gagccaggtg
      661 gccgagcgag tcgtggttgc ccatccgggc aagctgcggc tgatctaccg gtcacggcgg
      721 aagaacgacc gcaacgatgc gtaccgactc gctcgattga tgctggtcga cgaactgcca
      781 ccggtgcacg tgccgccggt ggacttccgg aactggcggt cgctgatcga gctgaggaat
      841 cggtacgtcg ggcaacgcac gcggatcaag aacagcctgc gtgcactgct gcgaacgtgc
      901 ggcgtcaagg gtccgcggag cctgtggtcg aagaaggggc tggcgtggct ggaggctctg
      961 cagctcgacg aggaccgggc gttcactcgg gcgatgctgc tcaacgatct cgccgcacag
     1021 cagcagaaca tcgcggagac cgagcggcgg ctgaagaaga tcgccagccg tcagccggcc
     1081 gtgcgtctgc tgatgaccat tcccggcgtc ggcatccgta ccgccgaggc gttcgtcgcc
     1141 tacacacacg acgcgaagcg tttccgtgga cgatcgatcg gtgcgtatct cggtctggta
     1201 ccgcggcagg actcgagcgg tgagacgaat cgcatgggcc gcatcacacg cgagggtccg
     1261 tcgacggtgc ggaagatgct ggccgaggcc acctggcagt cgatccgtcg gagccagcgg
     1321 gtgcggaggc acttcgaacg gatcatggga ggtcggcctg accgtcgcaa gaaggccttg
     1381 gtcgcgacgt cgcactacct ggcgcgagtg atgctggcga tgctcaagaa cggatccgtc
     1441 tggcgggagg acgtcgcgtc ggccgttgcg gcgtagcgtc cgatccgcaa gccacgctcg
     1501 agggtgcggg actcaagaag ccagacctcg ctgatgccga cttggacaga cagctgatcc
     1561 cgagcgagtg aatgaggctg gattcgaata cacccatatg gcgggtcctc atgcgacgac
     1621 gccgaataga tgtttggaac gatcccgcaa cgaaccctca cccttgacaa gggcttgctc
     1681 atagatgtcg gcccggaggg ccgtacggag cgctggcagg tgcgggcgat caggaccgcc
     1741 ggatcgaatc cggcggtccg tggaagagcg agtgtgggcc cggagggccc tacggagcgc
     1801 tcaaaaaagc aacgctcacg ggaggagcct ccgtgagcgt tgcgggatgc atggtggtgc
     1861 tc
//
LOCUS       DBNHOX010000685         1992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1149236, whole genome shotgun sequence.
ACCESSION   DBNHOX010000685 DBNHOX010000000
VERSION     DBNHOX010000685.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1149236"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="AC16UZ_14970"
     CDS             <1..244
                     /locus_tag="AC16UZ_14970"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6248036.1"
                     /translation="DPIERLKQHMIREGYWSQAQHEQLVEELQAAVDEAWQEALSYGS
                     MTEGPWLDRSEMFEDVFKDVPAHLLAQREQLRALEE"
     gene            258..1274
                     /locus_tag="AC16UZ_14975"
     CDS             258..1274
                     /locus_tag="AC16UZ_14975"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6248037.1"
                     /translation="MAQMNMIQALNSALNIMLSRDPGVIVIGQDVGFFGGVFRVTDGL
                     QRQYGEHRVIDTPIAEGGIVGSAIGMGVNGLRPVAEIQFADYIYPGCDQIISELARLR
                     YRTGGDFFAPVTIRTPCGGGIRGGQTHSQSPEGIFTHVCGLKVVMPSNPYDAKGLLIS
                     AIEDDDPVIFFEPKRIYNGPFDGDANKPAVPWSTHPKGEVPEDYYTIPIGRAEVIRPG
                     SEVTILCYGTMVHVCAAAAEAAEVDAEIIDVRTMAPLDVETLKTSVEKTGRCVIAHEA
                     TRFSGYGAELAATVSEECFYHLEAPIQRVAGWDTPYPHAFEWEYFPGQKRVATALERV
                     LEAP"
     gene            1271..>1992
                     /locus_tag="AC16UZ_14980"
     CDS             1271..>1992
                     /locus_tag="AC16UZ_14980"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR6248038.1"
                     /translation="MSEYVFKLPDLGEGTVEAEIVEWHVRPGDEVNEGDLLVDVMTEK
                     ASVEVPAPVTGTVLRTSGMPGDLIAVGAELAAIETGASQETTKAPSTVGSAIALPESP
                     DVSMRPEAVEAPIEDAVTHPEPSSPSSADSDQVATLPDTPVESARRVITSPAIRRRAK
                     EAGVDLESLTGTGPRGRILQRDLEQAIAGRETAAPPASGTGAVNEVKVIGVRRVIAKR
                     LQAAVREIPHFTYVEDVDVTAL"
BASE COUNT          396 a          632 c          649 g          315 t
ORIGIN      
        1 agatcccata gagcgtctga aacagcacat gattcgcgaa ggttactggt cacaggccca
       61 gcacgagcag ctggtagagg agttgcaggc cgctgtcgac gaggcctggc aggaagccct
      121 gagctacggc tccatgacgg agggtccgtg gctcgaccgc agcgagatgt tcgaggacgt
      181 tttcaaggat gttcccgccc acctgctcgc ccagcgcgaa cagctccgcg cgctggaaga
      241 atgaggaggt caccgacatg gcccagatga acatgatcca ggcgctaaac tcggctctga
      301 acatcatgct gagtcgagac ccgggcgtga tcgtcattgg ccaggacgtc ggtttcttcg
      361 gcggcgtttt ccgggtcacc gacggtctgc agaggcagta cggcgagcac cgcgtcatcg
      421 atacgcccat cgccgaagga ggcatcgtgg gcagcgccat cggcatgggc gtcaacggac
      481 tacggccggt agccgagatt cagtttgccg actacatcta cccgggttgt gatcagatca
      541 tcagcgagct cgcgcgcctg cggtatcgaa ccggcggcga cttcttcgcc ccggtgacga
      601 tcagaacgcc ctgcggaggc ggcattcgcg gcgggcagac ccactcccag agtccggagg
      661 gaatcttcac ccacgtctgc gggctgaagg tcgtcatgcc ctcaaaccct tacgatgcca
      721 aggggctcct gatcagcgcc atcgaggacg acgacccggt gattttcttc gagcccaaac
      781 gaatctacaa cggccccttc gacggcgacg cgaacaaacc ggccgttccg tggagcacgc
      841 accccaaggg cgaggtgccg gaagactact acacgatccc catcggccgc gccgaggtga
      901 tacggcccgg cagcgaagtg accatcctct gttacggcac catggttcac gtctgcgcgg
      961 cggccgcgga agccgctgaa gtcgacgccg agatcatcga cgtgcggacc atggcgccgc
     1021 tggatgtcga aacgctcaag acctcggtgg agaagacggg gcgctgcgta atcgcccacg
     1081 aagcaacgcg gttcagcggg tacggcgccg agctagccgc tacggttagc gaagagtgct
     1141 tttaccacct cgaagcaccc atccagcgag ttgccggttg ggataccccg tatccccatg
     1201 cattcgagtg ggagtatttt ccgggccaga agcgcgtcgc gaccgccctg gaacgggtac
     1261 tggaggcgcc atgagcgaat acgtattcaa gcttcccgat ctcggcgagg gaacggtcga
     1321 ggcggagatc gtcgagtggc atgtacgccc gggcgatgaa gtgaacgaag gcgatcttct
     1381 ggttgacgtg atgaccgaga aagccagcgt ggaggtcccc gcgccggtca ccgggaccgt
     1441 gctcagaacg tccggaatgc cgggcgattt gatcgccgtc ggcgctgagc tggccgccat
     1501 cgagaccgga gcctcccagg agacgacgaa ggccccgagc acggtagggt ccgccatcgc
     1561 ccttccggaa tcccctgatg tctccatgcg gcccgaggca gtggaggcac ctatcgagga
     1621 cgcggtcact catccagaac cctcgtcccc gtcgtcggcc gattcagacc aagttgccac
     1681 gctgcccgat acgccggtgg agagcgcaag gcgcgtgatc acgtcccccg ccatccgacg
     1741 acgggcgaag gaggccggcg tcgatctgga gtcgctcacg ggcacgggac cgcgcggacg
     1801 gatactgcag cgcgatctcg agcaggccat cgccggccgg gagacagcag cgcccccggc
     1861 gagcggcacg ggtgccgtaa acgaggtcaa ggtcatcggc gtgcgtcgcg tcatcgccaa
     1921 gcggcttcag gcagccgtgc gcgaaattcc gcacttcacc tacgtcgagg acgtggatgt
     1981 caccgccctg ga
//
LOCUS       DBNHOX010000686         1978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_441117, whole genome shotgun sequence.
ACCESSION   DBNHOX010000686 DBNHOX010000000
VERSION     DBNHOX010000686.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_441117"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..>1978
                     /gene="fusA"
                     /locus_tag="AC16UZ_14985"
     CDS             122..>1978
                     /gene="fusA"
                     /locus_tag="AC16UZ_14985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR6248039.1"
                     /translation="MSQPALKQLRNIGIISHIDAGKTTVSERILYYCGEIHKLGEVHD
                     GMATMDWMAQEQARGITITATSTTCRWGETWINLIDTPGHIDFTIEVERSLRALDGAV
                     AIFSAVEGVQPQSESVWHQADRYGVPRICLINKVDRIGADYRAVLEQMRQRLGARAAL
                     MQLPFGEEGSFDGVIDLLSRQLLTFDEDSLGAIVTVSAIPESLVEASAAAREALIEVI
                     ADWDDAVLADFLDGRDISAERALPALRKGVLAGQLFPVYLGSALRNRGIQPLLDAVAA
                     LLPSPLDLPPVSAHAADGEDVLLACDPAGPLCALAFKVLSDEGRKLTYLRLYSGTLAA
                     GDTVLNSTRGVEERASRLFRMHAHRRERIDTARAGDIVAVAGLKEALTGDTLCAPDQS
                     LLLAGLTIPEPVVSLAVEPRSVKDRDKLLLSLEKLQWEDPTFRVREDADTGQTVLTGM
                     GELHLEVVLRRLSDDFGVEVNAGQPQVVYRESVAGTARHCEVFAREIDGREQRGEVVL
                     AVAPGERGTGVEIVLPERLAETLSEDLRTALLETLRLASRAGVRVGYPMIDVIITVED
                     VPFVPGVTTALGVRAAAQRGLGQAALAAEPVLLEPVMALEISCPSEFSGKVLG"
BASE COUNT          322 a          611 c          656 g          389 t
ORIGIN      
        1 tcgaccggtt ttatgtcgag gagctggagc gtgaatattt cgagctcaac aatctcaacc
       61 tgaatgacct ctacctgatc cgcaaagggt atgcggcgta gccccgcgcc gctcgtcagc
      121 catgagccag ccagccctca agcagcttcg caacatcggc atcatctcgc atatcgatgc
      181 cggcaagacg accgtctcgg aacgaatcct ctattactgc ggtgagattc acaagctggg
      241 cgaagtccac gatggcatgg cgaccatgga ctggatggct caggagcagg ctcgcggcat
      301 caccatcacc gccaccagca ccacctgtcg ttggggtgaa acctggatca acctgatcga
      361 cacgccgggg catatcgact tcaccatcga agtggaacgc tcgctgcgcg cgctggacgg
      421 cgcggtggcg atcttcagcg ccgtggaggg cgtgcagccg cagagcgagt cggtctggca
      481 ccaggctgac cgttatggcg tgccgcgcat ctgtctgatc aacaaggtcg atcgcatcgg
      541 tgcagactat cgggcggtcc tggagcagat gcggcagcgt ctcggtgccc gcgcggctct
      601 gatgcagctg ccgtttggcg aggaagggtc cttcgatggg gtgatcgacc tgctctccag
      661 gcagctcctg accttcgatg aggactcctt gggtgcgatc gtgacggttt cggcaatccc
      721 ggagtcattg gtcgaggcat cggccgcggc tcgcgaggcc ctgatcgaag tcatcgccga
      781 ctgggacgat gccgtgctgg ctgattttct ggatggtcgc gacatctctg ccgagcgggc
      841 gctgccggca ttgcgcaagg gggttctggc cgggcagctg tttcccgtct accttggctc
      901 ggcgttgcgc aaccggggga ttcagccgct tcttgatgct gtcgccgcat tgctcccctc
      961 accgcttgat ctgccaccgg tttcggctca cgctgccgac ggtgaagatg ttctgctcgc
     1021 ctgtgatccg gccgggcctc tctgcgctct ggccttcaag gtcctgtcgg acgaagggcg
     1081 caagctcacc tacctgcgtc tttactccgg cacgcttgcg gctggagata ccgtgttgaa
     1141 cagtacccgc ggcgtcgagg agcgggcctc caggctgttt cgcatgcacg cccaccggcg
     1201 tgagcggatc gatacggcaa gggccggcga tatcgtggcc gttgccggtc tcaaggaagc
     1261 tctgaccggc gacacgctct gtgcgcccga ccagtcgttg cttctggcgg ggctgaccat
     1321 ccccgaaccg gtggtctccc tggcggtgga gcctcgctcc gtgaaggatc gcgacaaact
     1381 gctgctttcc ttggaaaagc tgcaatggga agacccgacc ttccgggtca gagaggacgc
     1441 cgacaccggt cagacggtgc tgaccggcat gggggaattg cacctcgaag tggtccttcg
     1501 ccggctttct gacgacttcg gtgtcgaagt caacgccggc cagccgcagg tcgtctatcg
     1561 cgaaagcgtt gcgggcacag cccgtcattg cgaggtcttt gcccgcgaga tcgacggcag
     1621 ggagcagcgg ggggaagtcg tcctggcggt tgctccgggc gaacgcggca ccggggtgga
     1681 aatcgtcttg ccggaaaggc tcgcggagac gctcagcgag gacctcagga cggctctcct
     1741 cgaaaccttg cggctggcat cgcgcgccgg ggttcgcgtc ggttacccga tgatcgatgt
     1801 gatcatcacg gttgaggatg tgccgttcgt tcccggcgtg accaccgcgc tcggggtgcg
     1861 tgctgctgcc cagcgcgggc ttggccaggc ggccctggcc gccgaaccgg ttctgctcga
     1921 gccggtgatg gccctggaga tttcctgccc gagcgaattc tccggcaagg tgctgggg
//
LOCUS       DBNHOX010000687         4984 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1276410, whole genome shotgun sequence.
ACCESSION   DBNHOX010000687 DBNHOX010000000
VERSION     DBNHOX010000687.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1276410"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1615)
                     /gene="glpK"
                     /locus_tag="AC16UZ_14990"
     CDS             complement(137..1615)
                     /gene="glpK"
                     /locus_tag="AC16UZ_14990"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIR6248040.1"
                     /translation="MSHLLALDQGTSSSKAIIFNLTGRVVGVGQHEFDNTFPADGWVE
                     QDPEVLWRTTLLAGREALASAGLKGEDIAAIGITNQRETTLVWDRQTGRCPHNAIVWQ
                     DRRTADRCELMRLDGLAEAVAETTGLLIDPYFSATKLAWILDNVSGAREQAESGALAF
                     GTVDTFLVWRLTKGRHHATDASNASRTMLFDIAGQTWSERLLNYFNIPAAMLPAVRDS
                     ADDFGVADPEWFGAPIPICGIAGDQQAALIGQGCFNPGMAKSTYGTGCFAMINTGAEQ
                     LRSQQQLLTTVAYRLDGEITFALEGSIFSAGVAVKWLRDQLGLVVDAADTEAAAIRAQ
                     GDTAGVFLVPAFTGLGAPHWAPEARGLVSGLTLATTRDQLITATLQAVAFQSHELIAA
                     MASDGAVVDNLRVDGGMVVNDWLCQCLADVVNLPVERPEITETTALGAAILAALGAGL
                     VEDLDSAARLWNLEREFLPAMDPARRESLLKGWRAAVARTLL"
     gene            2121..>4984
                     /locus_tag="AC16UZ_14995"
     CDS             2121..>4984
                     /locus_tag="AC16UZ_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HIR6248041.1"
                     /translation="MQSDNQPISGTATPSDPQATATAMATAQPLPPSIAATAPGEIKV
                     IKRNGAVVPYNDDKIAVALTKAFLAVEGGTAAASPRIRELVAELTLQVSATFKRRFPS
                     GGTIHIEDIQDQVELVLMRSGEHKVARDYVLYREERARARAADVVQPASNALNVVQED
                     GSREPLDLDRIRKLVGEACVSLSDVEADRIIDEAMSNMYDGISAKDVATALQIAARTL
                     VEEEPNYTYVTARLLLDELRTEAFRFLEVNAEGEHRATQSEVGGYYPAALAAFIEKGI
                     ELQLLDPVLATFDLAALGGAIKSERDLKFTYLGLQTLYDRYFIHSNDVRFELPQVFFM
                     RVAMGLAVQEDDINARAIEFYDLLSSFDYMSSTPTLFNSGTLRPQLSSCFLTTVPDDL
                     EGIYGAIRDNAMLSKWAGGLGNDWTPVRALGSYIKGTNGKSQGVVPFLKVVNDTAVAV
                     NQGGKRKGAVCSYLETWHLDIEEFLELRKNTGDDRRRTHDMNTANWIPDLFMQRVTEG
                     EDWTLFSPSDVRDLHDLYGQAFAKRYEQYEEMTRTGELKLFKRVKAPELWRKMLSMLF
                     ETGHPWITFKDACNVRSPQSHVGVVHSSNLCTEITLNTNGEEIAVCNLGSVNLVQHVR
                     DGELDTRKLKQTVRTAVRMLDNVIDINYYSVPQARNSNMRHRPIGLGIMGFQDALYKL
                     RIPYASEDAITFADRSMEAVSYYAIDASCKLAKERGAYASFSGSLWSRGILPIDSLKQ
                     LHQERGAQYLDANFDATMNWDKLRGRVQINGMRNSNVMAIAPTATIANITGVSQSIEP
                     TYQNLYVKSNLSGEFTVVNPYMVHDLKDRGLWDKVMVNDLKYYDGSVLSIDRVPDELK
                     QLYATAFEVEPRWLVDAASRRQKWIDQAQSLNLYIAGASGRKLDVTYRMAWLRGLTTT
                     YYHRSLGATSTEKSTLDRGRLNAVSATQADPTTAARAPTPL"
BASE COUNT          970 a         1635 c         1541 g          838 t
ORIGIN      
        1 ccctgcaaat ctcctagctg agctgaattc tagggaatct gcacgaagcc aatacaggac
       61 gtggtaccgc cttggcggcg ggaatcgcgc gccaggcccg cctttttggc taaaacgtga
      121 cgcgggacaa actttctcag aggagcgtcc tggccacggc ggcgcgccag cccttgagca
      181 ggctctcccg gcgtgccggg tccatggcag gcagaaactc ccgctcgagg ttccaaagcc
      241 ttgccgccga gtccagatcc tcgaccaacc cagcgccaag cgccgccagg atggcggctc
      301 ccagcgccgt ggtctcggtg atctcgggac gctccaccgg gaggttcacc acatcggcaa
      361 ggcactggca cagccagtcg ttgaccacca tgccaccgtc cacgcggagg ttgtcgacga
      421 cggctccatc cgaggccatg gcggcgatga gctcgtggct ctgaaaggcc acggcctgca
      481 gcgtggcggt gatcagctgg tcgcgggtgg tcgccagggt gagcccggac accagacccc
      541 gggcttcggg cgcccaatga ggtgctccga ggccggtgaa cgccggcacc agaaagacgc
      601 ctgcggtatc tccctgggcg cggatggccg cggcctccgt gtccgccgca tcgacaacca
      661 gcccgagctg gtctctcaac cacttgaccg cgacccctgc cgagaaaatg ctgccctcga
      721 gcgcgaaagt gatctccccg tcgaggcgat aggccacggt agtgagcagc tgctgctgcg
      781 agcgcagctg ctctgccccg gtgttgatca tggcaaagca gcccgtcccg taggtgctct
      841 tcgccattcc ggggttgaag cagccctgcc cgatcaacgc cgcctgctgg tcgccggcaa
      901 tcccgcagat cggtatcggc gcgccgaacc actccgggtc ggccacgccg aagtcatccg
      961 cgctgtccct gaccgcaggc agcatcgccg cggggatgtt gaagtagttg agcagccgct
     1021 cgctccacgt ctgcccggca atgtcgaaca gcatggtgcg tgacgcgttg gaggcatcgg
     1081 tggcgtggtg ccggcccttg gtaagccgcc agacgaggaa cgtatcgacc gtgccgaacg
     1141 ccagcgcgcc cgactcggcc tgctcgcgcg cgccggaaac gttgtcgaga atccaggcga
     1201 gcttcgttgc ggaaaaataa gggtcgatga gcaggcccgt ggtctcggcc accgcttccg
     1261 ccaggccatc gaggcgcatc agctcgcagc gatcggcggt gcggcggtcc tgccagacga
     1321 tggcgttgtg cgggcagcgg ccggtctggc gatcccagac cagggtggtt tcccgctggt
     1381 tggtgatgcc gatggcggcg atgtcctctc ccttcagacc cgcgctggcc agcgcttcgc
     1441 gccccgccag cagcgtggtc cgccacagaa cttccgggtc ctgttccacc cagccgtccg
     1501 ccgggaaggt gttgtcgaac tcgtgctggc ctacgcccac cacccgaccg gtgaggttga
     1561 aaatgatggc tttggagctg gaggttccct ggtcgagggc gagtagatgg ctcatgagga
     1621 gagtccggta ccacgaaaac cggccacaat atatagtggt cgccccggtt cggcgagagg
     1681 cgcgacggca gaaaatgtcc gccgtccggg tggcccacag catatccaca acgtatcccc
     1741 agctaattcc ttgcaaccct cctgcaatcg aattatcttg tgtggtcttg ggttcgaatc
     1801 ggctgaaaaa cccaacatgt tgcggttatg agcattgact cggtcaattt attgactgcc
     1861 agtctcaggg gagccagtcg ccgcagcagc aagtcagcga agcgcctgac cacgcgagcc
     1921 atacagaaat ctgttcggag gggacctatt tctgccggag ccgctaatac taggtattgt
     1981 gtttccacgg tcgccgccgc acgtccctct cgcggcacga ccggggcact gcgaccgtgg
     2041 aaacctgcgg tcgacccggt gcgagcgagt tcaacggttc aggcgttttt gaaacagcga
     2101 gcaggagcgc acaactagca atgcaaagcg acaaccaacc catttccggc actgccaccc
     2161 cgtccgatcc gcaggcgacg gcgaccgcca tggcaacggc ccagccgctg cccccgagca
     2221 tcgccgccac ggccccgggg gagatcaagg tcatcaagcg caacggcgcg gtcgtgccat
     2281 acaacgacga caaaatcgcc gtggctctca cgaaagcatt cctggccgtc gagggcggca
     2341 ccgccgccgc ctcaccgcga atcagagagc tcgttgccga gctgaccttg caggtctcgg
     2401 ccacgttcaa gcgccggttc ccgtccgggg ggaccatcca catcgaagac attcaggatc
     2461 aggtggagct ggtcctcatg cgctccggtg agcacaaggt ggcgcgcgat tacgttctct
     2521 accgcgagga gcgcgcgcgt gccagggccg cagacgtggt gcaaccagcc agcaacgccc
     2581 tcaacgtggt gcaggaagac ggcagccgcg agccgctcga cctggataga attcgcaagc
     2641 tggtgggcga agcctgtgtc tcgctcagcg acgtcgaggc cgatcggatc atcgacgagg
     2701 ccatgtccaa catgtacgac ggcatctcgg ccaaggacgt cgcgacggcc ctgcagatcg
     2761 cggcccgaac gctggtcgag gaagagccga actacaccta cgtgaccgct cgcctgctgc
     2821 tcgacgagct gaggaccgag gcgttccgtt ttctcgaagt caatgccgaa ggcgagcaca
     2881 gggcgaccca atccgaggtc ggcggctact atcccgcggc gctggccgcc ttcatcgaga
     2941 agggcatcga gctgcagctc ctggatccgg tgctggcgac attcgacctt gcggctctcg
     3001 gcggagccat caagtcggaa cgggacctca agttcactta cctgggcctg cagacgcttt
     3061 acgaccgcta cttcatccac tcgaacgacg tgcggttcga gctgcctcag gttttcttca
     3121 tgcgcgtcgc catggggctc gccgtgcagg aagacgacat caacgcccga gccatcgagt
     3181 tctacgacct gttgtcgtcg ttcgactaca tgagctccac gccaacgctg ttcaattccg
     3241 gaacgctccg gccgcagctg tcgtcgtgct tcctcacgac ggtgccggac gatctggaag
     3301 gcatctacgg agcgatccgg gacaacgcca tgctgtcgaa gtgggcgggc ggtctcggca
     3361 acgactggac tccggtgcgg gcgctgggct cctacatcaa gggaaccaac ggcaagtccc
     3421 agggcgtcgt gcctttccta aaggtggtca atgacacggc ggtggccgtg aaccaggggg
     3481 gcaagcgcaa gggcgcggta tgttcctatc tggagacctg gcacctggac atcgaggagt
     3541 tcctcgagct gcgcaagaac acgggcgacg accggcgccg cacccacgac atgaacacgg
     3601 ccaactggat tccggatctg ttcatgcagc gcgtgaccga aggtgaggac tggacgctgt
     3661 tctcgcccag cgacgtgagg gaccttcacg acctttacgg gcaggcgttt gccaagcgct
     3721 acgagcaata cgaggagatg acgcgcaccg gggagctcaa gctgttcaag cgggtcaagg
     3781 ctccggaatt gtggcgcaag atgctctcga tgctgttcga gacgggccat ccgtggatca
     3841 ctttcaagga cgcctgcaac gtgcgttcac cgcagtccca cgttggcgtg gtgcactctt
     3901 ccaacctctg cacggaaatc accctgaaca ccaacggcga agagatcgcg gtgtgcaacc
     3961 tcggctcggt gaacctggtg cagcacgtcc gcgacgggga gctcgacacg cgcaagctca
     4021 agcagaccgt tcgcaccgcg gtgcgcatgc tcgacaacgt gatcgacatc aactactact
     4081 cggtgcccca ggcgcggaac tccaacatgc ggcatcgccc catcgggctc ggcatcatgg
     4141 gtttccagga tgctctgtac aaactgcgta taccttacgc cagcgaagat gccatcacct
     4201 tcgccgaccg ttcgatggaa gccgtcagct attacgccat tgacgcttcc tgcaagctgg
     4261 ccaaggagcg cggtgcctac gcgagcttct ccggctcgct gtggagccgc ggcatcctgc
     4321 ccatcgattc gctgaagcaa cttcatcagg aacgtggcgc ccagtacctg gatgccaatt
     4381 tcgacgccac catgaactgg gacaagctgc gcggccgcgt gcagatcaac ggcatgcgca
     4441 actcgaacgt catggccatt gcccccacgg caaccatcgc caacatcacg ggcgtctccc
     4501 agtccatcga gcccacgtat cagaacctgt acgtgaaatc gaacctgtcg ggcgagttca
     4561 ccgtggtcaa cccgtacatg gtccacgacc tgaaagaccg cggattgtgg gacaaggtga
     4621 tggtcaacga tctcaagtac tacgacggca gcgtgctgtc catcgaccgg gtgcccgacg
     4681 agctaaagca gctctacgcc acggccttcg aggtggaacc gaggtggctg gtggacgccg
     4741 cgagccgccg gcagaagtgg atcgaccagg ctcagtcact caacctctac atcgccggag
     4801 cgtccggccg gaagctggac gtgacctacc gaatggcgtg gctcagaggg ctcacgacca
     4861 cctattacca ccgctccctg ggcgccacca gcacggagaa atcgaccctc gaccgcggca
     4921 ggctcaacgc cgtgtcggcc actcaggccg acccgaccac cgccgctcga gcgccgacac
     4981 cgct
//
LOCUS       DBNHOX010000688         3754 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1469153, whole genome shotgun sequence.
ACCESSION   DBNHOX010000688 DBNHOX010000000
VERSION     DBNHOX010000688.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1469153"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="AC16UZ_15000"
     CDS             <1..537
                     /locus_tag="AC16UZ_15000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6248042.1"
                     /translation="PNLYYRRVREFVMTAEARPEMMAHMNSLSNHMACEDTAALFAFA
                     SADRAARDGPAGCVGYCMSGPFAFAAAAAFPDRIAAAASIHGVRLCTDAIDSPHLNAE
                     HVGGELYFACAEHDDWAPPEMIEALEGHLSRVGASYRIEWYPGTSHGFVFPARPVYHP
                     AAAERHWERVLDLFSRNL"
     gene            complement(521..1591)
                     /locus_tag="AC16UZ_15005"
     CDS             complement(521..1591)
                     /locus_tag="AC16UZ_15005"
                     /inference="COORDINATES: protein motif:HMM:NF014653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HIR6248043.1"
                     /translation="MLIDRDALTRFVSEIFVRLDAEASDAQEVAAHLVSANLKGHDSH
                     GVGMVPTYVGNIRSGALALNTHARLVRDKGAVMLIDGQMGFGQVVGREAVELAIERVR
                     QTGVVAAGVRNCHHLGRIGHYGEICGRAGYVSMHFVNVVGHQPLVSPFGGRQARISTN
                     PFCCVVPRSDEPPVVLDMATSAIAQGKVRVAYMKGDKVPDGSLIDHEGKPTNDPSVMF
                     EDPRGSLGPFGHHKGYGLALMCELLGGGLAGEWTAQAPPPDRFTIVNHMLMVVLDPDL
                     FGGAEAFHREVEEMIAYLRSTMPAAGVDRVRVPGEPELESMAERAAGGIPIDDNTWAA
                     IRKSAQVSGMTEEEIAGFTGSG"
     gene            1672..2529
                     /locus_tag="AC16UZ_15010"
     CDS             1672..2529
                     /locus_tag="AC16UZ_15010"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR6248044.1"
                     /translation="MDKCAEGFSLIEGPVWDPDRGLLFSDVLVGGVFCLGGDGTVTTV
                     FEHRRGIGGMALHEAGGLIVSGRNLSFKPYDEGSTVTVLDRDEENGLVGFNDLTTDAE
                     GRIYAGSLGASPVFADGREPRAGDLYLVDLDGSARVVAEDVRLTNGLGFSPDGTTLYH
                     SDSGRRTVFRYDVQPDGSLSAKEPFVTPDNGVPDGLAVSRDGAVWVALARGGHGVAVY
                     EASGRLREFIEIPLPMCTSVCFGGEDLKDLYIVSGSAGAGSDRAGAVFRERVDVAGLI
                     MTPARVRLA"
     gene            2597..3427
                     /locus_tag="AC16UZ_15015"
     CDS             2597..3427
                     /locus_tag="AC16UZ_15015"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6248045.1"
                     /translation="MAIEPMDTGTEDLLAHQEDGVAVITLNRPQARNAMSAAMNAGLA
                     KALDEAERNPAVRCVVITGAGGAFCAGGDVKGMAARSQGGGPGPTVDERIHLQRLNQR
                     ATAGRIYQMPKPVLASLPGPAAGAGLALALAADLRIMADSAFITTAFAKVGFSGDFGG
                     SFFLSRLVGSGKAKELYFLSDRVSAAECERLGIANAVVPAEELEAQTMALARRLASGP
                     PVAYRYMKENFNRAAAGAGVLECLDLEATHHVHTGLTEDHRAAAKAFVEKTEVEFKGR
                     "
     gene            complement(3465..>3754)
                     /locus_tag="AC16UZ_15020"
     CDS             complement(3465..>3754)
                     /locus_tag="AC16UZ_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR6248046.1"
                     /translation="GIGNSRVTDNLAGTCIDDAVQVTDRDAVNMVYRELREEGWFFGS
                     STGINLCAAVQVAHSMGPGHTIVTVLCDTGAKYQSRLFNPEWLAERGLTPG"
BASE COUNT          595 a         1223 c         1262 g          674 t
ORIGIN      
        1 ccgaacctct actaccggcg cgtccgggag tttgtcatga ccgccgaagc ccggccggag
       61 atgatggccc acatgaactc gctttcaaac cacatggcct gcgaggacac cgccgctctg
      121 tttgccttcg catcggcaga ccgagcggcg cgggatggcc cggccggctg cgtgggctac
      181 tgcatgagcg gacccttcgc ctttgccgcg gctgccgcct ttcccgatcg cattgccgcg
      241 gccgcctcca ttcacggcgt gaggctttgc acggacgcga tcgattctcc ccatctgaac
      301 gcggagcacg ttgggggcga gctttacttc gcctgcgccg agcacgacga ctgggctccc
      361 ccggagatga tcgaagccct ggaggggcac ctttcccggg tgggcgccag ctaccggatc
      421 gagtggtatc cgggcacttc tcatggcttc gtcttccccg cacggcccgt ctaccacccg
      481 gcggcagccg agcggcattg ggaaagggtt ctcgatctct tcagccggaa cctgtgaacc
      541 cggcgatttc ctcttccgtc atgccggaca cctgggccga tttccggatc gcggcccagg
      601 tgttgtcgtc gatgggtatg cccccggccg cgcgctcggc catggactcg agctcgggtt
      661 ccccgggcac ccgcaccctg tcgacgccgg cagccggcat cgtgctgcgc aggtaggcaa
      721 tcatctcctc tacctcccga tggaaggcct ccgcgccgcc gaacaggtcc gggtccagca
      781 cgaccatgag catgtggttg acgatggtga accggtccgg aggaggtgcc tgggccgtcc
      841 actctccggc gagcccgcca ccgagaagct cgcacatcag ggcgagcccg taacccttgt
      901 gatgtccgaa tggccccagc gagccccggg ggtcttcgaa catcacgctg ggatcgttgg
      961 tcggttttcc ttcgtggtcg atcagggatc cgtccggcac cttgtctcct ttcatgtagg
     1021 ccacccggac cttgccctga gcaatggcgc tggttgccat gtcgagcacc acagggggct
     1081 cgtcgctcct cggcaccacg cagcagaaag ggttggtgct gatgcgcgcc tggcgcccgc
     1141 cgaacggcga gaccagcggt tggtgtccca ccacgttgac gaagtgcatc gacacgtagc
     1201 ccgctcgccc gcagatctcg ccgtagtgcc cgatgcggcc caggtggtgg cagttgcgca
     1261 cgccggccgc gacgacgccc gtctgcctga cccgctcgat ggcgagctcg acggcttcgc
     1321 gccccaccac ctggccgaaa cccatctgcc cgtcgatgag cataacggcg cccttgtccc
     1381 ttaccagcct ggcatgggtg ttcagcgcga gagcgcctga gcggatattg ccgacgtagg
     1441 tcggcaccat gccgacccca tgggaatcgt ggcctttgag attggcggac accagatggg
     1501 cggcgacctc ctgcgcgtcg ctggcttcgg cgtcgagccg aacgaagatt tcgctgacga
     1561 agcgcgtcag tgcatcgcgg tctatgagca tggtggcttc tcatcgtgtt ggaacgctca
     1621 gtgttgcctc tttaaagagc ctgtgacaag gtctgacgag agggggttgg catggacaag
     1681 tgcgcggaag ggttcagcct gatcgaaggg ccggtctggg atccggaccg cggcctgctt
     1741 ttcagcgacg tcctggtcgg tggggtcttc tgcctcggcg gcgacggcac ggtgacgacg
     1801 gttttcgagc atcgtcgggg catcggcggc atggccctgc acgaagccgg tggcctgatc
     1861 gtcagcggcc ggaatctgtc tttcaagccc tacgatgagg gctcgacggt caccgttctc
     1921 gatcgcgacg aggagaacgg cctggtgggc ttcaacgatc tcaccaccga tgccgaaggg
     1981 cgaatctacg ccggcagtct gggagcgagc cccgtgttcg ccgacgggcg tgagccgcgt
     2041 gcgggggatc tctacctggt cgatctggac ggcagcgccc gggtcgtggc cgaggacgtt
     2101 cggctgacca acgggctggg gttctctccg gacggtacca cgctctacca ctccgacagc
     2161 ggacgccgaa cggtcttccg ctacgacgtg cagccggacg gttcgctctc ggcaaaggag
     2221 ccgttcgtta cgccggataa cggggtgccc gacgggctcg cggtatcccg cgacggcgct
     2281 gtctgggtgg cgcttgcccg gggcggtcac ggcgtagccg tctatgaggc gtccggccga
     2341 ctgcgtgagt tcatcgagat tcccctgccc atgtgcacca gcgtgtgctt tggcggcgaa
     2401 gatctgaagg acctttacat cgtctccggg tccgcgggcg ccgggtctga cagggcggga
     2461 gccgtcttcc gagagcgtgt cgatgtggcc ggcctgatca tgacaccggc tcgggtgcgg
     2521 ctcgcgtgac gaaggcgtcg ccgtcgacct gcaggcaggt gcgtcttcat cggttagagc
     2581 ataggagttt gcaggcatgg cgatagaacc catggacacg ggaaccgagg atctcctcgc
     2641 acatcaggaa gatggcgtcg cggtgatcac gttgaaccgg ccccaggcac gcaatgccat
     2701 gtcggccgcg atgaacgcag gcctggcaaa ggctctggac gaggccgagc ggaatcccgc
     2761 cgtgcgctgt gttgtcatca ccggagccgg cggcgcgttc tgcgccggcg gagacgtgaa
     2821 aggcatggcg gcgcgaagcc aggggggcgg ccccgggccc accgtggacg agcgcattca
     2881 ccttcagcgc ctgaaccaga gggcgacggc cggccgcatt taccagatgc cgaagccggt
     2941 tttggcgagc cttcccggac cggctgcagg agcggggctg gctctcgccc tggcagccga
     3001 tctgcgcatc atggccgact ccgccttcat cacgacggcc ttcgcgaagg tgggcttcag
     3061 cggcgacttc ggtggcagct tcttcctctc tcgcctggtt ggcagcggga aagccaagga
     3121 actctacttt ctttccgatc gggtgagtgc cgctgagtgc gagcggctgg gaatcgccaa
     3181 cgcggtggtg ccggcggaag aactcgaggc ccagacgatg gcgcttgcgc ggcggctggc
     3241 gtccggaccg cccgtggcat accggtacat gaaggagaac ttcaaccggg ccgccgcagg
     3301 tgccggggtg ctcgagtgcc tcgacctcga ggccacgcat cacgtgcaca ccggtctcac
     3361 ggaggatcat cgcgcggcgg caaaggcctt cgtcgagaaa accgaggtcg agttcaaggg
     3421 ccgatgaggc gttcctccag gagccgcggc gctcgggcgc gtcctcagcc cggtgtcaga
     3481 ccccgttcgg ccagccactc ggggttgaac agtcgggact ggtacttggc accggtgtcg
     3541 caaagcacgg tgacgatcgt gtgtcctggc cccatcgagt gggcaacctg aacggcggcg
     3601 cacaggttga tccccgtgct cgaaccgaaa aaccaaccct cctcccgcag ctcgcggtag
     3661 accatgttca cggcgtcccg gtcggtcacc tgaaccgcgt cgtctatgca ggtaccggcc
     3721 aggttgtccg ttacccggct gttgccgatc cctt
//
LOCUS       DBNHOX010000689         1636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_377703, whole genome shotgun sequence.
ACCESSION   DBNHOX010000689 DBNHOX010000000
VERSION     DBNHOX010000689.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_377703"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="AC16UZ_15025"
     CDS             <1..429
                     /locus_tag="AC16UZ_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HIR6248047.1"
                     /translation="RRCISYLTIELKEAIPEPLRAPIGNRIFGCDDCQIYCPWNRFAG
                     VTNEPDFQPRHGLEAATLLDLFGWSPETFERNTRGSAIRRISWDQWRRNLAVALGNGS
                     PSAAALGALREARAGASAMVCEHIDWAIKRLTRRSGGSEP"
     gene            complement(434..979)
                     /gene="orn"
                     /locus_tag="AC16UZ_15030"
     CDS             complement(434..979)
                     /gene="orn"
                     /locus_tag="AC16UZ_15030"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HIR6248048.1"
                     /translation="MAMPTNLVWMDLEMTGLDPETDVIIEMATVITDSDLNILAEGPV
                     LAVWQPQAKLDAMDEWNQTHHGESGLIDRVRASAVSEREAEARTLAFIEGHVERKQAP
                     LCGNTIWQDRRFLTRYMPTLEGYLHYRNIDVSSLKELVARWRPELLKGINKANRHTAL
                     SDIRESIEELRYYRKHFIALS"
     gene            1165..>1636
                     /locus_tag="AC16UZ_15035"
     CDS             1165..>1636
                     /locus_tag="AC16UZ_15035"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR6248049.1"
                     /translation="MVHVGDAAGYLQGHVPGAVLVEPGELIGGAPPATGRLPSRERLE
                     ALFSRIGFDPDQEVIVYDDEGGGWAGRMAWTLDVVGHTRWTYLNGGIHSWLAAGLPVE
                     TGPGLPVPPANVTIDIQGEPIAELEDVMAAIEDDDQVVWDVRSREEYLGLRSGSR"
BASE COUNT          267 a          526 c          553 g          290 t
ORIGIN      
        1 cgccggtgca tctcctatct caccatagag ctcaaggagg cgattcccga gccgcttcgc
       61 gcgcccatag gaaaccgcat cttcggctgc gacgactgcc agatctactg cccgtggaac
      121 cgctttgccg gcgtcaccaa cgagccggac ttccagccac gccatgggct cgaagcggca
      181 acgctgctgg acctgttcgg ctggtccccg gagaccttcg agaggaacac gcgcggttcg
      241 gccattcggc gcatcagctg ggatcagtgg cgccgaaacc tcgccgtggc gctgggcaac
      301 ggctctccca gcgcggccgc tctgggggcc ctgcgggaag cgcgggcggg cgcatcggcc
      361 atggtttgcg aacacatcga ctgggcgatc aaaagattga cccgacgatc gggcggatct
      421 gagccgtgag ccgtcaggac aacgcgatga agtgcttccg gtagtaacgc agctcctcga
      481 tggattcccg aatgtcggac agcgcggtgt gccgattggc tttgttgatc cctttcagaa
      541 gctccggccg ccagcgtgcc accagctcct tgaggctgct cacgtcgatg ttgcggtaat
      601 gcaggtagcc ctcaagggtg ggcatgtagc gggtgagaaa ccgcctgtcc tgccagatgg
      661 tgttcccgca caggggcgcc tgctttcgct cgacgtggcc ctcgatgaag gcaagggtcc
      721 gcgcctctgc ctcccgctcg gagaccgctg aggcccgcac gcgatcaatg agccccgact
      781 cgccgtggtg ggtctggttc cactcgtcca tggcgtccag ctttgcctgg ggttgccaaa
      841 cggcgagcac cgggccctcg gcgagaatgt tgagatcgct gtcggtgatc accgtggcca
      901 tttcgatgat gacgtcggtt tcagggtcga gacccgtcat ctcgagatcc atccacacca
      961 ggtttgtcgg cattgccact cctaaacgga tgcgggtcgc ccgcccgccg ccggccattc
     1021 tgccacaagc ctgggatctg ccacggcgat tcagttctcg gctaagatag gcggcccttt
     1081 tgccccggac aggaagccgt catcgcgcca ccgagaatca tcgaacccga ggagctggcc
     1141 ggcgccgacc cggagggccg gctcgtggtg catgtgggcg atgcggccgg ctacctgcag
     1201 gggcacgttc ccggcgccgt tctcgtcgag cctggggagc tgattggcgg cgcgccgccg
     1261 gctaccggcc ggctgccgag cagggagagg ctggaagcgc ttttctcgcg aatcggtttc
     1321 gatccggacc aggaggtcat cgtctacgac gacgagggcg gcggctgggc cggccggatg
     1381 gcgtggacgc tggatgtggt gggccacacc cgctggacct acttgaacgg cggcatccac
     1441 agctggctgg ccgccgggct gccggttgag accggaccgg ggctgcccgt gccgccagca
     1501 aacgtgacga tcgacattca aggcgaaccc atcgcggagc tcgaggacgt catggctgcc
     1561 atcgaggacg acgatcaggt ggtctgggac gtccgctctc gcgaggagta tctggggctg
     1621 cgcagcggtt cccgtc
//
LOCUS       DBNHOX010000690         3758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_120517, whole genome shotgun sequence.
ACCESSION   DBNHOX010000690 DBNHOX010000000
VERSION     DBNHOX010000690.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_120517"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="AC16UZ_15040"
     CDS             complement(<1..454)
                     /locus_tag="AC16UZ_15040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6248050.1"
                     /translation="MSAWLATPVGERLLADEAPYVAETARRFHGDTLLWAGCHTPLTD
                     TVRGCMVRNRVFALDGSSPAGEGLTTFRCELDAIPLANNALDGVLLHHALEMAEDPRT
                     ALRELSRVLAAGGRLTVVGFNPFSFWGVRIAYARVFRDSFSGLRLVSPA"
     gene            529..2076
                     /locus_tag="AC16UZ_15045"
     CDS             529..2076
                     /locus_tag="AC16UZ_15045"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HIR6248051.1"
                     /translation="MTATAPVVRFASTLRTLMFVILASLVGCQTNPIPEAAETAAAEP
                     PPPVAVPPPVIEPTLTYELKPDLWRDIRERFELAHSLDQKRVQQELRWLRENPRYLHN
                     LKPRMERFLAYIHGEVAARELPGELALLPIVESALDPYAFSHGGAAGLWQFIPATAKR
                     FGLQRNWWYDGRRDPIAATQAALDYLEYLHLLFEDWHVAVAGYNAGEGNVRRAQRKAA
                     PGATFWEMALPRETQMYVPRLLALAAVVTDPEAYGLTLPELSPDVPFVAVDTQGQFDL
                     AKAADTLSMDLDALYDWNPALNQWSTPPDGPHRLMIPALEPALSQAALAAVPAGERVQ
                     WLRIKVADGDTLSEIAHRHRTDTATLRRVNNLTGSSIRAGQAMYIPKSDSAPSRSPRS
                     RAQKGLEYAVQPGDSLWTISRAHGVSMTRIMKANHVGPKDLLKVGQTLTIPGAQRSTP
                     AAGEDGREVIRTVRYGVRKGDSLARIAARFNVRVSEIAQWNQLDVDNYLQPGQSLKLH
                     VNVAAGQ"
     gene            complement(2107..>3758)
                     /locus_tag="AC16UZ_15050"
     CDS             complement(2107..>3758)
                     /locus_tag="AC16UZ_15050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6248052.1"
                     /translation="RLQAAVLDQLIARQLLGQAVDELGFGVSQSRVDEAVTANPAFQV
                     DGRFDAELYKRRVRQLRYTPQQFHRETGALMALEQLRRGLTETAFLTDWEVRQSARLL
                     SQKRDIAYLAFTEDDFLPLVEVSDEEVATRYAENELEYRTEETVDVEYLELSAEGLVN
                     DESISVSEAAIEETYAADSAEALSGDRRQARHILLEVGASRTAEQAENELNAARERIV
                     GGADFASLAEELSEDPGSAALGGDLGMVAKGIFDPAFEQALWELEVGEISEPVRTDFG
                     YHLIQLDAVDVRELPPLSEQRDDIELRLRREQAEELFSERLREFDNLAFEQPDGLEGI
                     AAALALETQSVAGVSRSRGDGVFANASLRDAVFASEVLDNGYNTQAVALTDNRAVVAR
                     VKARYEPEAIPLDDVRDDIRAAIASEKARIALRQAQQNALARVRSGESVSAVADDYGL
                     GWQTFELVSRSSADAPRAVLSAAFSLPRPGEREKSVGEATLDDGSVAVVTVTRVVDGD
                     VSVMAEREIESMRGFLGNRVASLDFAGLTTTLEREASISTP"
BASE COUNT          668 a         1302 c         1163 g          625 t
ORIGIN      
        1 gagcggggct gaccagccgc agcccgctga agctgtcgcg aaagactcgc gcgtaagcga
       61 tccgaacgcc ccagaaactg aacgggttga accccacaac cgtcagccgc ccgccggccg
      121 caagcacgcg ggagagctcc cgcagcgcgg tgcgcgggtc ctcggccatc tccagcgcat
      181 ggtgaagcag cacaccatcc agggcgttgt tggccagagg tatggcgtcc agctcgcagc
      241 gaaaggtcgt gaggccctcg cccgccgggc tcgaaccatc gagcgcgaac acgcgattgc
      301 gaaccatgca tccgcgcacg gtatcagtca ggggggtatg gcaccctgcc cagagcagcg
      361 tatcgccgtg aaagcggcgg gccgtttccg ccacgtaggg cgcctcgtcg gcaaggaggc
      421 gctcaccgac cggtgtagcc agccaggcgg acatctgatg acgcaaggat cttgccaatc
      481 gcacagggat accgcaaagt tgaaggatca ttctgaaggc accactcgat gaccgcaacc
      541 gcacctgtcg ttcggtttgc ctcgaccctg cgcacgctga tgtttgtgat cctggcgtcg
      601 ctcgtggggt gccagaccaa tcccatcccc gaagcggccg aaaccgcggc cgccgagcct
      661 cccccgcccg tcgcggttcc gcctcccgtc atcgagccga cgctcaccta cgagctgaaa
      721 cccgatctgt ggcgcgacat ccgcgagcgc tttgagctgg cccacagcct cgaccagaaa
      781 cgcgtgcagc aggagctgcg ctggctgcgc gagaatccgc gctacctgca caatctgaaa
      841 ccccgaatgg agcggttcct cgcctacatt cacggggagg tggccgctcg ggagcttccc
      901 ggtgagctcg ccctgctccc catcgtggag agcgcgctcg acccgtatgc gttttctcac
      961 ggcggcgccg ccgggctgtg gcagttcatc cccgccacgg cgaagcgatt cgggctgcag
     1021 cgaaactggt ggtacgacgg ccgtcgggat cccatcgcgg ctactcaagc cgccctcgac
     1081 tacctcgaat atcttcatct gcttttcgag gactggcatg ttgccgtcgc gggctacaac
     1141 gctggagagg gcaacgtccg ccgcgctcag cggaaagccg caccgggggc caccttctgg
     1201 gagatggctc tgccccggga aacccagatg tacgtgccgc gcctgctggc gttggcagcg
     1261 gtggtaaccg accccgaggc ttacggcctg acgctgcccg agctttcgcc ggacgtgccg
     1321 ttcgtggccg tcgacacgca gggacagttc gacctcgcca aggcagccga caccctttcc
     1381 atggatctcg acgcgctcta cgactggaat ccggccctga accagtggtc gacgccgccg
     1441 gatggtcctc accggctgat gattcccgca cttgaaccag cactgagcca ggcggccctt
     1501 gcggccgttc ccgctggcga gcgggtccag tggctgcgga tcaaggtggc agacggagac
     1561 accctcagcg aaatcgccca ccgtcaccga acggacacgg ccaccttgcg acgggtgaac
     1621 aatctgacgg gctcctcaat tcgtgccggc caggctatgt acattcccaa atcggattcg
     1681 gcccccagcc gcagtccccg cagccgcgct cagaaaggac tggaatacgc ggtgcagccc
     1741 ggagattccc tgtggaccat cagtcgggcg cacggggttt cgatgacgcg catcatgaaa
     1801 gccaatcacg tggggcccaa ggacctgctg aaggtaggcc agactctcac cattcccggc
     1861 gctcagcgct cgacgcccgc tgcgggagaa gacggacggg aagtaattcg cacggtgcgc
     1921 tacggcgtgc gcaagggcga ctcgctggcg cgcatcgccg ctcgcttcaa cgtgcgggtc
     1981 agcgagatcg cgcagtggaa ccagctggac gtggacaact acctgcagcc cggccagtcg
     2041 ctgaaactgc acgtcaacgt agcggctgga cagtaactga ccagccggcc gccggacggg
     2101 gtcgacctac ggcgtgctga tcgacgcttc cctttccaga gtggtcgtca gccccgcgaa
     2161 atccagagaa gccacgcgat ttcccaggaa gccgcgcatg ctttcgatct ctctttccgc
     2221 catcacggaa acatccccgt cgaccacacg ggtaacggtg accaccgcaa cggagccgtc
     2281 atcgagcgtg gcttcaccca cgctcttctc ccgctcaccg ggccgtggca ggctgaaggc
     2341 cgccgagaga acggctcgcg gggcgtcggc cgagctgcgg ctgacgagtt cgaaggtctg
     2401 ccaacccagg ccgtagtcgt cggccaccgc ggaaacgctc tcgccggaac gaacgcgcgc
     2461 cagcgcgttc tgctgggctt gtctgagcgc aatccgcgcc ttttccgacg cgatggccgc
     2521 gcgtatgtca tcgcgaacgt catccagggg aatcgcctcc ggctcgtatc gggccttgac
     2581 gcgggcgacc accgcgcggt tgtccgtcag cgctaccgcc tgtgtgttgt agccgttgtc
     2641 cagaacctcg ctggcgaata ccgcatccct caaggacgcg ttggcgaaaa caccgtcgcc
     2701 gcgcgaacgt gatacgccgg ccaccgactg agtctccagc gccagggccg cggcgatacc
     2761 ctcgagaccg tcgggttgct cgaaagccag attgtcgaac tcgcgcagac gctcggagaa
     2821 gagctcttcg gcctgttccc gccgcagccg cagctcgatg tcatcgcgtt gctcggacag
     2881 cggcggcagc tcccgcacgt cgacggcgtc cagctggatc aggtggtagc caaagtcggt
     2941 tctgaccggc tcggaaatct cgccgacctc gagctcccag agcgcttgct cgaacgccgg
     3001 atcgaaaatg cccttggcca ccatcccgag atcgccgccc agcgcggccg atccaggatc
     3061 ctcgctgagc tcctcggcca gggacgcgaa gtccgcgccg cccacgattc gctctcgcgc
     3121 cgcgttgagc tcgttctcgg cctgctccgc cgtccggctg gcgccaactt ccagcaggat
     3181 gtggcgcgcc tgtcggcgat cgccggaaag agcctccgcc gaatccgccg cataggtttc
     3241 ctcgatggcg gcctcgctca ccgagatgga ctcgtcgttc accagccctt ctgcactgag
     3301 ctcgagatac tcgacgtcga cggtctcctc ggtgcggtat tccagctcgt tctcggcgta
     3361 gcgcgttgcg acctcctcgt cggaaacctc gacgagaggc agaaagtcgt cttcggtgaa
     3421 cgccaggtaa gcaatgtccc gcttctgact cagcagccga gcgctctgcc tcacttccca
     3481 gtccgtcagg aaagccgttt cggtaagccc tcgccgaagc tgttccagag ccatgagggc
     3541 acccgtttct ctgtggaact gctggggcgt gtagcgcagc tgacgtaccc gtctcttgta
     3601 gagctcggcg tcgaatcggc cgtctacctg aaaggcgggg ttcgcggtga ccgcttcgtc
     3661 cacgcgactc tgggacacgc cgaaaccgag ctcgtccacg gcctgcccca gcagctgcct
     3721 ggcaatcagc tgatccagca cggcggcctg tagacgtg
//
LOCUS       DBNHOX010000691         3571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_377835, whole genome shotgun sequence.
ACCESSION   DBNHOX010000691 DBNHOX010000000
VERSION     DBNHOX010000691.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_377835"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="AC16UZ_15055"
     CDS             complement(<1..609)
                     /locus_tag="AC16UZ_15055"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6248053.1"
                     /translation="MNQKEIDELRQLMEYEAARSEPPEGFPSLPDLPGGRYTDQRFYD
                     LEQQHIWRKSWLLAGHIDEIPEPGCFRLWETAGQPVVLVHADSGAVNAFYNTCSHRGA
                     PVVTEPEGKRPRLVCKYHGWTYSHDGELLAIRDPEDFRDLDFSCRGLRPIRCERFGNL
                     IFVNFDDGAPTLLEWLGPVAAEWEEFQFDKCRLASRHIFDLNC"
     gene            complement(659..1423)
                     /locus_tag="AC16UZ_15060"
     CDS             complement(659..1423)
                     /locus_tag="AC16UZ_15060"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6248054.1"
                     /translation="MSGRFDGKVVMITGATSGLGKHAAVAFAREGARVVASGRRQAEG
                     EETLAEIEAAGGTAVFVQADVSVSEDVARIVAAARDRFGGLDWAYNVAGVPGDAFAKA
                     AEYSEDAWDETMEINVKGVFLCVKHAIPAMLERGGGAIVNMSSIAGIGGSPGGVAYVA
                     SKHAIIGLTKAAALDYAEQNIRINALAPGVIYTDMLAWGITQTPGLEADLISQHPIGR
                     LGQLEEVTNAAMWLCSDEAGFVTGTTLTVDGGHLAR"
     gene            1607..2053
                     /locus_tag="AC16UZ_15065"
     CDS             1607..2053
                     /locus_tag="AC16UZ_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248055.1"
                     /translation="MVKASVWVVGLLVVAAGGYYAYLQLVGNPRVMTEIRAEPDGERA
                     RRVMLIDLPDGRTLPVNYLREDDMVYMGVDGLWWRQFRQGDVPVELLIRGEDLQGHAR
                     AVLDDPDRTRRVFARLRPTAPDWLPGRMGGVLVEIRLQAATLDEED"
     gene            2103..2804
                     /locus_tag="AC16UZ_15070"
     CDS             2103..2804
                     /locus_tag="AC16UZ_15070"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR6248056.1"
                     /translation="MKEFLSARAVEFVSINVLEDREARERLARGGIRTLPVVSRDDAF
                     VPGMDLARVAEFLGLDYQGGNILPADVLVSRYHRVLDAAVRYVGQIPPARLHDTLPHR
                     DRSYLALGNHLVQIAVDFLSVTRGAALTGRLAESVPETELEPPALMAAAAQAKRGLAD
                     WLAAADSAELASPVVTYFGSQTLHQVLERAVWHSAQHGRQLMMVLQRLGISPDGPLTD
                     EDFAGLPMPEEVWDG"
     gene            2897..>3571
                     /locus_tag="AC16UZ_15075"
     CDS             2897..>3571
                     /locus_tag="AC16UZ_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6248057.1"
                     /translation="MQLPTDEEWARACAADGEFGLAARHWTGGLTLAVGEAELSLRLT
                     DGAVSPGRAESAEGLLTYAGPADVWEQVLAAQPPRFHNDIMANLTVGGGLNRQVDPVV
                     HAQYYPAVMRAVELLRPAEPPFMPMRDEARPPGAFDAPVGRYVHLELEGHDHRIYFEE
                     AGQGIPLLMQHTAGCHGSQWRHLFEIPEITSRFRLIAYDLPYHGKSVPPVSREWWAER
                     YELKGDF"
BASE COUNT          522 a         1171 c         1250 g          628 t
ORIGIN      
        1 gcagttcagg tcgaagatgt ggcgggaggc gagccggcac ttgtcgaact gaaactcttc
       61 ccactccgcg gcgaccgggc ccagccactc cagcagcgtg ggggccccgt cgtcgaagtt
      121 cacgaagatc aggttgccga agcgctcgca gcggatgggc cgcaggccgc ggcaggagaa
      181 atccagatcg cggaagtcct cgggatcgcg gatggccaga agctcgccgt cgtggctgta
      241 ggtccagccg tgatatttgc acaccagacg cgggcgtttg ccttccggtt cggtgacgac
      301 gggcgcgccg cggtggctgc acgtgttgta gaaggcgttc accgcgccgc tgtccgcatg
      361 aacgagcacc acgggctggc ccgcggtctc ccagaggcgg aaacagccgg gttccggaat
      421 ctcgtcgatg tgaccggcga gcagccacga ctttcgccag atgtgctgct gctccaggtc
      481 gtagaagcgc tgatcggtgt aacgaccgcc cggcaggtcg ggcagtgacg gaaaaccttc
      541 gggcggttcg cttcgtgccg cctcgtactc catgagctgg cgcagctcgt cgatttcttt
      601 ctggttcatg gattcagcct ccctcgtcgt gccatcgatg gttctacgat gcttcgtttt
      661 agcgggccag gtgcccgccg tccacggtca gggtggtgcc cgtgacgaag ccggcctcgt
      721 ccgagcacag ccacatggcc gcgtttgtca cttcctctag ctgccccagc cgcccgatcg
      781 gatgctgtga gatcagatcg gcctccaggc cgggcgtctg ggtgattccc caggcgagca
      841 tgtcggtgta gatcacgccc ggcgccaggg cgttgatcct gatgttctgc tcggcgtagt
      901 ccagcgctgc ggccttggtg agcccgatga tggcgtgctt ggacgccacg taagccacgc
      961 cgcccggcga gccgccgatt cccgcgatgg acgacatgtt gacgatggcg ccgcccccgc
     1021 gctcgagcat ggccgggatg gcgtgcttta cgcagagaaa aacgcctttg acgttgatct
     1081 ccatggtctc gtcccaggcg tcctcggaat actctgccgc cttggcaaag gcgtcgccgg
     1141 gaacgccggc cacgttgtag gcccagtcga gcccgccgaa tcggtcccgg gcagcggcca
     1201 cgatgcgcgc aacgtcctcg ctcacgctga cgtcggcctg gacgaacacg gccgtgccgc
     1261 cggcggcttc gatctccgcg agggtctctt cgccttccgc ctggcgtcgt ccggacgcta
     1321 cgaccctggc cccttcccgg gcgaaggcga cggccgcgtg cttacccaga ccggacgtgg
     1381 cgccggtgat catgacgacc ttgccgtcga agcgtccgct catcgtttgc tccccaagca
     1441 ctctggcgcc aattgtgccc gaggccctgc gtacccgcta tctcatcaag gccggattcc
     1501 acggccccgg gcgagagtct gccggagcgc ccggcggttc agctttgcgc tgccctgtgt
     1561 caggctacgt gctctgagtt cccggggcgg gtcggacttg ccgaaagtgg tcaaagcgtc
     1621 ggtgtgggtc gtcgggctgc tggtcgttgc ggccggaggc tactatgcgt acctgcagct
     1681 cgtcggcaat ccccgggtga tgaccgaaat ccgggccgag ccggacggcg agcgggcacg
     1741 gcgcgtcatg ctcatcgacc tgcccgacgg ccggaccctg ccggtcaact acctgcgtga
     1801 ggacgacatg gtctacatgg gggtggacgg gttgtggtgg cggcagtttc gccagggcga
     1861 tgtgcccgtt gagctgctca tccgtggtga agatctgcag ggccacgccc gtgccgtgct
     1921 cgacgatccc gaccggaccc gcagggtctt cgcccggctc cgacccaccg cgccggactg
     1981 gcttccgggc cggatgggcg gcgtgctggt cgagatccgg ctgcaggcgg cgactctcga
     2041 tgaggaggac tgacgtgggg acctgcgtct actggcagcc cgggtgaacc agctgtctcc
     2101 gggtgaagga gtttctatcg gcccgtgcgg tcgagttcgt ttccatcaac gtgctggagg
     2161 accgggaagc gcgtgagcgc cttgcccgtg ggggtatccg gaccctgcct gtggtttccc
     2221 gcgacgacgc tttcgtgccc gggatggatc tcgcgcgggt cgccgaattc ctgggcctgg
     2281 actatcaggg cggcaacatc ctgccggcag acgtgctcgt cagccggtat caccgggtgc
     2341 tcgatgccgc ggtgcgatac gtggggcaga taccgcccgc ccgcctgcac gacacgctgc
     2401 cccatcggga ccgttcctac cttgccctgg gcaatcatct cgtccagatc gcggtggatt
     2461 tcctgtcggt gacccggggt gctgcgctca ccggccggct tgccgagtcg gtgccggaga
     2521 ctgagctgga gccgccagca ctaatggccg ccgccgccca ggcgaagcgc ggcctggcgg
     2581 actggctggc ggcagcggac agtgccgagc tcgcttcacc tgtggtgacg tacttcggtt
     2641 cccagacgct ccatcaggtg ctggaacggg cggtgtggca ctcggcccag catgggcgtc
     2701 agctgatgat ggttctgcaa cggcttggga tctcgccgga cgggccgctg acggacgagg
     2761 atttcgcagg gctgcccatg ccggaggagg tctgggacgg ctgaccgccg cggcggtggt
     2821 gcgtggggtt atgatgatgg cgggcgtccg gtgacggtcg cccagcggcg agtcgaacgg
     2881 ggaggagaac gggggaatgc agcttccaac cgacgaggaa tgggcccgcg cgtgcgcggc
     2941 ggatggcgag ttcggtctgg cggcccggca ctggacgggg ggtctcaccc tcgccgtcgg
     3001 ggaggcggag ctcagccttc gcctgacgga cggcgccgtg tctccgggca gggccgaatc
     3061 ggccgaaggt ctgctgacct acgcgggccc tgccgacgta tgggagcaag tgctcgcggc
     3121 ccagccgccg cggtttcaca acgacatcat ggccaacctg accgtggggg gcgggctcaa
     3181 ccgccaggtc gacccggtgg ttcacgctca gtactacccg gcagtgatgc gcgccgtgga
     3241 gctgcttcgg ccggcggagc cgccgttcat gcccatgcgc gacgaggccc gaccgcccgg
     3301 ggccttcgac gcccccgtgg ggcgctatgt ccatctggag ctggagggcc acgatcaccg
     3361 gatctacttc gaggaggcgg ggcagggcat ccccctgctg atgcagcaca cggccggctg
     3421 ccacggcagc cagtggcgcc acctcttcga gattcccgag atcaccagcc gcttccgcct
     3481 catcgcctac gacctgccgt atcacggcaa gtcggtcccc cccgtcagcc gcgagtggtg
     3541 ggcggagcgc tacgagctca agggagattt c
//
LOCUS       DBNHOX010000692         2515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_827662, whole genome shotgun sequence.
ACCESSION   DBNHOX010000692 DBNHOX010000000
VERSION     DBNHOX010000692.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_827662"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="AC16UZ_15080"
     CDS             complement(<1..253)
                     /locus_tag="AC16UZ_15080"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6248058.1"
                     /translation="MPKSPEYPELEAARAGWRFQPEDDCLRGKVILVTGAGDGIGAAA
                     ARTYACYGADVVLLGRTRSKLETVFDWIEAETDTRPVIVP"
     gene            complement(237..929)
                     /gene="ubiG"
                     /locus_tag="AC16UZ_15085"
     CDS             complement(237..929)
                     /gene="ubiG"
                     /locus_tag="AC16UZ_15085"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HIR6248059.1"
                     /translation="MNVDQAEIGKFDALAARWWDPEGEFKPLHDINPVRLNYLMEHCD
                     IRSRPVVDVGCGGGILAESLAREGATVTGIDMAESPLAVARLHAEDSGVQVEYLATTA
                     EDLAAERAGRVGTVACLEMLEHVPDYASTVKACADLAAPDGKLVFSTINRNPKAYLLA
                     ILGAEYVLGLLPRGTHDYDKFIRPAELAAAVRAAGLTVKEIIGMRYNPFTRQCSLTPD
                     VDVNYMLYAEKS"
     gene            complement(926..2245)
                     /locus_tag="AC16UZ_15090"
     CDS             complement(926..2245)
                     /locus_tag="AC16UZ_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HIR6248060.1"
                     /translation="MPSTPCDLIIDADWILPIVPAGSVLQEASIAIADGRILKIGDRE
                     LIHGEHQPREVHNLPGHALLPGLINAHGHAAMTLLRGFAEDAPLENWLSDHIWPTEAR
                     LVDADFVRDGARLAMAEMLKTGTTCFSDMYFFPEVTAAEARAAGMRAQIAFPVIEFPN
                     AWSASSDEGFHKGLALHDDYRRDPLIRVAFGPHATYSVSEGDLERILVYSEELDAHVQ
                     IHLHETAREVAEARDRVGMSWLRYLDERGLLGPNLQAVHATHLDDEEIDLLAENNVHV
                     VHCPLSNLKLASGICATTTLLARGVNVALGTDGAASNNGLDLLLEARLATLLAKLRDG
                     DAGALPAHRALEMATLGGARALGMEAQIGSLEPGKAADMVAIDLTGLKFQPLFDPVAQ
                     LVHTTSGDGVAHVWVAGRQLLRDGQLCTLDEQAILRAARGWRERISQ"
BASE COUNT          381 a          840 c          845 g          449 t
ORIGIN      
        1 aggggacgat caccggccgg gtgtccgtct cggcctcgat ccagtcgaac acggtttcca
       61 gcttggatcg cgtgcgcccc agcagcacca cgtcggcacc gtagcaggcg tacgttctcg
      121 ccgccgccgc gccgatcccg tcgccggctc cggtgaccag gatcaccttg cctcgcaggc
      181 agtcgtcttc cggctggaac cgccagccgg cgcgcgccgc ctccagctcg gggtactcag
      241 gacttttcgg catagagcat gtagttcacg tcgacgtcgg gcgtcaacga gcactgccgt
      301 gtgaacgggt tgtagcgcat accgatgatc tctttcacgg tcagccctgc ggcgcgaacg
      361 gccgccgcga gctccgcggg gcggatgaac ttgtcgtagt cgtgggtgcc tctgggcagc
      421 aggcccagga cgtactcggc gcccaggatg gccagcagat acgcctttgg attgcggttg
      481 atggtggaga aaaccagttt tccatcgggt gctgcgaggt ctgcgcaggc tttcaccgtg
      541 gaagcgtagt cggggacgtg ctcgagcatt tccaggcagg ccaccgtgcc gaccctgccg
      601 gcgcgctccg cggccaggtc ctcagcggtg gtagccaggt actccacttg aacgccgctg
      661 tcctccgcgt gcaggcgggc cacggccagg gggctctccg ccatgtcgat gcccgtcacc
      721 gtagcgccct cccgagcgag gctttccgcc agaatgcccc cgccgcagcc cacgtctacc
      781 accgggcgcg agcggatgtc gcagtgctcc atgagatagt tgagccgcac ggggttgatg
      841 tcgtggagcg gcttgaattc cccttcggga tcccaccagc gcgcggccag ggcgtcgaat
      901 ttgccgatct ccgcctgatc cacgttcatt gggatattcg ctcccgccac ccgcgcgccg
      961 cccgcaggat ggcttgttcg tccagcgtgc acagctgacc gtcgcgcagc agttgccgcc
     1021 ccgccaccca gacgtgggcc accccgtctc cggacgtggt gtggaccagt tgcgcgaccg
     1081 gatcgaacag gggctggaat ttcagcccgg tcaggtcgat ggcgaccatg tccgcggcct
     1141 tgcccggttc cagcgaaccg atctgcgctt ccatgcccag ggctcgcgcg ccgccgaggg
     1201 tcgccatctc cagtgcccga tgggccggca gggcgcccgc gtcgccgtct ctgagcttgg
     1261 ccagcagcgt tgccaggcgt gcttccaaga gcaggtcgag gccgttgttc gacgcggcgc
     1321 cgtcggttcc cagggccacg ttcacccccc gggcgagcag ggtcgtggtg gcgcagatgc
     1381 cgcttgccag cttgaggttc gacagcgggc aatggaccac gtgcacgttg ttctctgcga
     1441 gcaggtcgat ctcctcgtcg tccaggtgcg tggcgtggac ggcctgcaga ttcggcccga
     1501 gcagcccgcg ctcgtcgaga tagcgcagcc agctcattcc gacccggtcg cgagcctcgg
     1561 cgacctcccg cgcggtctcg tgaaggtgga tctgcacatg ggcgtcgagt tcctcggagt
     1621 acacgaggat ccgctcgagg tcgccctcgg agacgctgta cgtggcgtgg ggcccgaacg
     1681 cgacccggat gagagggtcg cgccggtagt cgtcgtggag cgccaggccc ttgtggaacc
     1741 cctcgtcgga gcttgcgctc cacgcgttgg gaaactcgat gacgggaaag gctatctggg
     1801 cccgcatgcc ggccgcccgc gcctccgcag ccgtcacctc gggaaagaag tacatgtccg
     1861 agaagcaggt cgtgccggtc ttgagcattt cggccatggc cagccgggcg ccgtcgcgga
     1921 cgaagtccgc atccacgagc cgagcctcgg tgggccagat gtggtcgctc agccagttct
     1981 ccagcggcgc gtcctccgca aagccgcgca gcagcgtcat ggcggcgtgg ccatgggcat
     2041 tgatcagccc cgggagcagc gcgtggccgg gcaggttgtg cacctcccgg ggctggtgct
     2101 cgccgtgaat cagctcgcga tcgccaatct tcaggatcct gccgtcggca atggcaatgc
     2161 tcgcctcctg gagcaccgag cctgcaggca cgatgggcag tatccagtcg gcatcgatga
     2221 tcagatcgca tggggtggac ggcatcggcc gattatagcc cctccggccg ggcgtaggcg
     2281 ggttcataga gcgcggagac gggcggaatt ccgtggtcaa aagtatcagg ttcgcctgcg
     2341 cttcgtggta aaatggcgcg cttttctgca gcggttccat atgtccgacc tggggtccga
     2401 tcagggccgg gagatcctcc cggtcaacat cgaagacgag cttcgacagt cttatctcga
     2461 ttacgccatg agcgtgatcg tcgggcgcgc ccttcccgac gttcgtgacg gcctc
//
LOCUS       DBNHOX010000693         1860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1342017, whole genome shotgun sequence.
ACCESSION   DBNHOX010000693 DBNHOX010000000
VERSION     DBNHOX010000693.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1342017"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..697)
                     /locus_tag="AC16UZ_15095"
     CDS             complement(137..697)
                     /locus_tag="AC16UZ_15095"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HIR6248061.1"
                     /translation="MDFGAWPVWAGAALIFVARVCDVTLGTLRISFISRGEKSLAPAV
                     AFVEMIIWLFAISQVVLNLTNLAYYFAYAGGFASGVFVGLRIEERLAIGKRIIRTVME
                     GDGTDVVAAVKSAGFGVTSVRGTGGSSDVSVIFSVVDRGDVRRYMQVVEAHQPAAFVS
                     VEDVSSVHQGVFPSRRPMFVFPAWRK"
     gene            complement(1077..1319)
                     /locus_tag="AC16UZ_15100"
     CDS             complement(1077..1319)
                     /locus_tag="AC16UZ_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248062.1"
                     /translation="MAMFILAVGVLLLGIKLVTRQLPPASTPDQDRRLAEQAEEIELL
                     RDEVRRLQDQADFTERLLSERSDVHPGDGPERDAPG"
     gene            complement(1390..>1860)
                     /locus_tag="AC16UZ_15105"
     CDS             complement(1390..>1860)
                     /locus_tag="AC16UZ_15105"
                     /inference="COORDINATES: protein motif:HMM:NF015999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HIR6248063.1"
                     /translation="SLGIDADTFSHAPVFTVDEAKEKRGRLDGAHTKNLFVRDKKGAM
                     WLIVALEDRAVDLKALARTLGHRGFSFGSEARLMGYLGVTPGAVTPFGVVNDHSGAVR
                     VALDRGLLDHDVWNFHPLDNGMTTRLRGADMLRFLEAVDHPPTWIELGGVAGDG"
BASE COUNT          331 a          672 c          579 g          278 t
ORIGIN      
        1 caagaggggt gggttttttt ggccgtcctc cggctcggag tgaggccacg tagtcgcaca
       61 gatgctctcc gcagggtgcg acggcctccg ccccaagccg ggagccggcc tttgtggtcg
      121 acccaacgcc gtggcattac ttgcgccagg ctggaaagac gaacatgggg cgacgggagg
      181 ggaacacgcc ctggtgcact gagctgacat cctctaccga aacgaaggcg gccggctggt
      241 gcgcctccac tacctgcatg tagcggcgca catccccgcg gtcaacgacg ctgaagatca
      301 cgctcacgtc gctcgaaccg cccgtcccgc gaacactcgt gacgccgaag ccggccgact
      361 tgacggcagc gacaacatcc gttccatcac cctccatcac cgttcggatg atgcgcttgc
      421 cgatcgcgag ccgctcctcg atccggaggc cgacgaacac gccggacgcg aagcccccgg
      481 cgtaggcgaa gtagtaggcc aggttcgtga gattcagcac cacctgactg atcgcgaaca
      541 gccagatgat catctcgacg aaggccactg cgggcgcgag gctcttctcg ccccgcgaga
      601 tgaacgagat ccgaagcgtc ccaagcgtaa cgtcgcagac tctcgccacg aaaatgaggg
      661 cggccccggc ccataccggc cacgcaccga agtccatcaa caagtcacac ctccgaagga
      721 ccctacacgc agcgacgcgg ccaggttggg ccacggtcca agtgcctggc ccaccaaggg
      781 accaggatcc caagaagata gcgcgccccg ccccggccac cgaggctaca acccgcgccg
      841 gctccattcc ccgatcgggc agatgtcgcc cgtcaccttc gagcggctcc aacgcgagac
      901 cgaccaggtc gaggccgccc gatgccgcta ccttcgtcgg cccgtcgccc gctaccgcgg
      961 caagcgtggc tctccggagc ctctaggacc agctacaaac tcgagtccct gtgggcgcac
     1021 gtgagatcgc ctcgccgcgt ctcctccgaa cgggagccgg tgtggagccg cccagctcag
     1081 cccggcgcgt ctcgctcagg accgtctcca ggatggacgt cgctgcgctc gctcagcagc
     1141 ctctcggtga agtcagcctg atcctgcagg cgccgcactt cgtcccgcag cagctcgatt
     1201 tcttcggcct gctcggccag acggcgatct tggtccggcg tcgacgcagg aggcagctga
     1261 cgggtgacga gcttgatgcc cagcaggagg actcccaccg cgaggatgaa catcgccatc
     1321 atcacgattc ccatggcctc aagttagccg tgcgtgccga gcaggacgta ggcctccgcc
     1381 cgcgacggct catccgtccc ccgcgacacc ccccagctcg atccaggtcg gcgggtgatc
     1441 gaccgcctcc aggaagcgga gcatgtcggc gccgcgcagc cgggtcgtca tgccgttgtc
     1501 gagggggtgg aagttccaca cgtcgtggtc gagcagcccc cgatccaggg ccacgcgaac
     1561 ggcgccggag tggtcgttga ccacgccgaa gggcgtgacc gcgccggggg tcacccccag
     1621 gtagcccatg agccgcgcct cgcttccgaa cgagaagccc ctgtggccga gggtcctggc
     1681 gagcgccttc aggtccaccg ccctgtcctc gagcgcgacg atcagccaca tcgcgccctt
     1741 cttgtcgcgc acgaagagat tcttggtgtg ggctccgtcc agccggccgc gcttctcctt
     1801 cgcctcgtcg accgtgaaga ccggggcgtg cgagaacgtg tccgcgtcga taccgagcga
//
LOCUS       DBNHOX010000694         4026 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1790938, whole genome shotgun sequence.
ACCESSION   DBNHOX010000694 DBNHOX010000000
VERSION     DBNHOX010000694.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1790938"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="AC16UZ_15110"
     CDS             <1..523
                     /locus_tag="AC16UZ_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248064.1"
                     /translation="HRVLATYPENVIATQGLDEVVSQLRGTANQLLAAGDLGALQELV
                     DRSSAVGLDPVAVNEMKSSLETEIARRATVAERLDQARGLLAEGFITEPQGRNVVALL
                     REVERLDPGNEPAKRMLKESAERLASVAQEAYEVGMVDDAKHYLELALTVTPDVAAWR
                     ELRATWEKSVASN"
     gene            507..1640
                     /locus_tag="AC16UZ_15115"
     CDS             507..1640
                     /locus_tag="AC16UZ_15115"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR6248065.1"
                     /translation="MLQVTDLQYFISFHDDEFPLEDSLTIGRHLDNDVIVAGEDVLDY
                     HLRLEPTPRGPKVHPLGEASLILNGHHYSESRGLVAGDELVVGQNVLGVGVRRLHPAE
                     AESWSLHGDAAHGVHEMVGDLSVGRGEDSGLVVADDHVSREHALFTLADDVVWVRDLD
                     SANGTFVNGERVSGGCRLYHGDMIAFDQFEFQLIGQGADLTPVRAREGMQEGAMIKES
                     GPARSDTTEFAVVANADAVAELAPPAREKGTFLLGAAEPVLGITYRLRMGKTSLGRDD
                     ACDVVVKDRTVSSRHAEVVLRPEGATITNLMATNGTRVNGREIQSAQLADGDIIGLGR
                     VRFVYRSVTEGRRAARLRPLHRWLIAGAAIVAAVLAGLLIDTL"
     gene            1664..2566
                     /locus_tag="AC16UZ_15120"
     CDS             1664..2566
                     /locus_tag="AC16UZ_15120"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6248066.1"
                     /translation="MGVHIPYRRDLEFTYGEVAEIAPGIRRVIAENPSPFTLYGTGTY
                     ILGTGNVAVIDPGPADPAHIAAIVAGLQGETISHVLVTHTHMDHSPGCALLREHTDAP
                     TYAFGPHGAGKLEEGVPVEEGGDMEFVPDVVVRHGDVVEGADWSVECVYTPGHTSNHM
                     CFQLREQKALFTGDHVMGWSTSIISPPDGDMFAYMKSLDLLLERDDAVYWPTHGPCID
                     APKTHVRAFIEHRREREEQILKCVSRGVGQIRDMVPIMYVDTPEFMYPAAARSVLAAV
                     EGLVARGDLACDGAVSLDGNYRLT"
     gene            complement(2563..>4026)
                     /locus_tag="AC16UZ_15125"
     CDS             complement(2563..>4026)
                     /locus_tag="AC16UZ_15125"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6248067.1"
                     /translation="VHVTQTLPKAGLESFNLVVLRSPEVLNIIQGLGYSGARILVIDA
                     QQRVRAETGSNRPIEETDPASPTFVLLRDGFERVRPFLHWVSTGEDYLPVKSRPEDSE
                     GTADVAITTSLTGDPIALRRALSENNEIIIAAHPIVSRDAVIGTVVVEQNIDDILSFQ
                     RSALEQVILLSIASLFAVFIALLAFAGRLAWRIRNLRQEASSAIDQYGRLKISELHNE
                     MKAGDEIGDLARSVSNMLSKLHQHNNFLENMPRTLRHEINNPLNTLSTSLQNLAEENP
                     EIQDSKYMESAKRGVNRIGSIVQNLADAANLEESLEAEELEVVDIAQLLENYVANCRI
                     THPTCEFVFRGPNRPVLSRVSDFRIEQLLDKIIDNAIDFHRSNSPIVVQLDAYRDHLQ
                     ITVANRGPILPAKAEKSLFDSMVSHRGQQNRLHFGLGLYVVRVIADHHGGFVRALNLA
                     DGSGVAIVVQLPTVETTEGTPGRQRDAEDIEPGVAAS"
BASE COUNT          684 a         1268 c         1354 g          720 t
ORIGIN      
        1 tcatcgggtg ctcgcaacgt accccgagaa cgtcatcgcc acacaggggc tcgacgaggt
       61 ggtttcccag ctgcgcggga ccgccaacca gctgcttgcc gcgggtgacc tgggggcgct
      121 gcaggagctc gtcgaccgtt ccagcgccgt aggcctcgac ccggtagccg tcaacgagat
      181 gaagagcagc ctcgaaacgg agatcgctcg gcgcgcgacc gtcgccgagc ggctcgatca
      241 agcgcgcggg ctgctggcgg agggattcat cacggagccc cagggacgca acgtggtcgc
      301 tctgctgcgc gaagtcgagc gtctcgaccc gggcaacgag ccggccaaac gcatgctcaa
      361 ggagtccgcc gagcgtcttg ccagcgtggc gcaggaagct tacgaagtgg gcatggtgga
      421 cgacgccaag cactatctcg agctggcgct gacggtgacg ccggacgtcg cggcctggcg
      481 agagcttcga gcgacgtggg aaaagagtgt tgcaagtaac tgatctgcaa tattttattt
      541 cttttcatga cgacgaattt ccgctcgaag acagcctgac catagggcgt catctcgaca
      601 acgacgtcat cgttgccggt gaggacgtgc tcgactacca cctgcgcctg gaacccaccc
      661 cacgggggcc gaaagtccac ccgctggggg aggcaagcct gattctcaac ggtcatcatt
      721 actccgaatc ccggggcctg gtggccggcg acgagctggt cgtcggccag aacgtgctcg
      781 gggtaggggt taggcgcctg catccggcgg aagccgagag ctggtcgctc cacggtgatg
      841 ccgcacacgg cgttcacgag atggtcggag acctgagcgt gggacgcggc gaggacagcg
      901 gcctggtcgt cgccgacgac cacgtctccc gcgagcacgc gctcttcacg ctggccgatg
      961 acgtggtttg ggttcgggat ctcgattcgg ccaacggcac cttcgtcaac ggcgagcgcg
     1021 tctcgggagg ctgccgtctg tatcacggcg acatgatcgc gttcgatcag ttcgagtttc
     1081 agctcatcgg gcagggggcg gatctgaccc ccgtgcgtgc gcgcgaaggc atgcaggaag
     1141 gggccatgat caaggagtcc gggccggctc gctcggatac caccgagttt gccgtcgttg
     1201 ccaacgccga tgccgtagcc gagctggcgc cgcccgcccg tgaaaagggg acgtttctgc
     1261 tgggcgcggc agagcccgtc cttgggatca cctaccgtct gcgcatgggc aagacgtcgc
     1321 tcgggcgtga cgatgcctgc gatgtcgtgg tcaaggatcg cacggtctcg tcgcgccacg
     1381 cggaggtcgt cctgcgtccg gagggcgcca ccatcaccaa cctgatggcg accaacggaa
     1441 cccgggtgaa cgggcgcgag atccaaagcg cccagctcgc cgacggcgac atcatcgggc
     1501 tcgggagggt gcgtttcgtc tatcgcagcg tcacggaggg caggcgcgct gcgcgcctgc
     1561 gtccgctgca ccgttggctg atagcgggcg ccgccatcgt tgcggccgtc ctggcaggtc
     1621 ttctcatcga caccctctag cgcgatggat tatgcttcgc ctcatgggcg tacacatacc
     1681 gtatcgacgc gatctggagt tcacctacgg tgaggtcgcc gagatcgcgc cgggaatccg
     1741 ccgcgtcatc gccgagaatc ccagcccgtt caccctctac ggtaccggca cgtacattct
     1801 gggcaccggc aacgttgcgg tgatcgatcc cgggccggcc gatccggccc acatcgcggc
     1861 catcgtcgcc ggccttcaag gcgaaaccat ttctcacgtg ctggtgaccc acacccacat
     1921 ggatcactcc ccgggctgcg cgctgctgcg tgagcacacc gatgcgccaa cttatgcctt
     1981 cgggcctcac ggcgccggca agctcgagga gggcgtcccc gtggaggagg gcggcgacat
     2041 ggagttcgtg cccgacgtgg tggtccgcca cggtgacgtc gtcgagggcg ccgactggtc
     2101 cgtggagtgc gtctacacgc ctggccacac ctccaatcac atgtgtttcc agctgcgcga
     2161 gcagaaagct cttttcaccg gcgaccatgt catgggctgg tcgacgagca tcatctcgcc
     2221 accggatggc gacatgttcg cctacatgaa aagcctcgat ctgctgctcg agcgcgatga
     2281 cgccgtctac tggcccaccc acgggccctg catcgatgcg ccgaaaaccc acgttcgggc
     2341 cttcatcgag catcgcaggg agcgggagga gcagattctg aagtgcgtga gccggggcgt
     2401 cgggcagatc cgcgacatgg tgcccatcat gtacgtcgac actcctgagt tcatgtatcc
     2461 ggccgcagcc cgcagcgttc tggctgcggt ggaagggctg gtggcccggg gcgacctcgc
     2521 ctgcgacggc gcggtctctc tcgacggcaa ctaccgactg acttagctcg ccgctacccc
     2581 gggctcgata tcctccgcgt cccgctgacg acccggcgtt ccctccgtcg tctcgactgt
     2641 gggcagctga acgacgatgg cgactccgga gccatcggcc aggttgagcg cccgcacgaa
     2701 cccgccgtgg tggtcggcaa tgacccggac cacgtacagg ccgaggccga agtgcagccg
     2761 gttctgctgt ccccggtgac tgaccatgga gtcgaaaagc gacttttccg ccttggcggg
     2821 aaggatgggg ccgcggttag ccaccgtgat ctgcagatgg tcacgatagg catcgagctg
     2881 aaccacgatg gggctgttgg agcggtgaaa gtcgatggcg ttgtcgatga tcttgtcgag
     2941 cagctgctcg atgcggaagt cggacacccg gctgagcacc ggacgattgg gcccgcgaaa
     3001 cacgaactcg cacgtcgggt gggtgatgcg gcagttcgcc acgtagtttt ccaggagctg
     3061 agcgatgtcc accacttcga gctcctcggc ttccagcgac tcttcgagat tcgcagcgtc
     3121 cgccagattc tgcacgatgg agccgatgcg gttgacgccg cgcttggcgc tctccatgta
     3181 cttgctgtcc tgtatctcgg ggttttcctc ggccaggttc tgcagggacg tgctgagggt
     3241 gttcagcggg ttgttgatct cgtgccggag cgtgcggggc atgttctcca ggaagttgtt
     3301 gtgctggtgc agcttggaca gcatgttgga aacgctgcgc gcgagatcgc cgatctcgtc
     3361 gccggccttc atctcgttgt gcagctcgct gatcttcaga cgcccgtact gatcgatggc
     3421 cgagcttgcc tcctgacgca ggtttcggat gcgccatgcg aggcggccgg caaaggcgag
     3481 cagggcgatg aagacggcaa acaggctggc aatggacaac aggatcacct gctccagggc
     3541 cgaccgctgg aaggagagga tgtcgtcgat gttctgctcc accaccacgg tgccgatgac
     3601 ggcgtctctg gagacgatgg ggtgcgcggc gatgatgatc tcgttgttct cgctcagcgc
     3661 ccggcgcaga gcgatggggt cgccggtgag cgaggtggtg atcgccacgt ccgccgtgcc
     3721 ttcggaatcc tccgggcggc tcttcacggg caggtagtcc tcgccggtgg agacccagtg
     3781 cagaaaaggc cgcacccgct cgaagccgtc gcgcagcagg acgaacgtcg ggctcgcagg
     3841 atcggtctcc tcgatggggc ggttgctgcc ggtctccgcg cgaacccgct gctgggcgtc
     3901 gatgaccagg atgcgggcgc cggaataccc caggccctga atgatgttca gcacctcggg
     3961 cgagcgaagc accaccaggt tgaagctctc cagccccgcc ttgggcaggg tctgggtgac
     4021 gtggac
//
LOCUS       DBNHOX010000695         4055 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_699205, whole genome shotgun sequence.
ACCESSION   DBNHOX010000695 DBNHOX010000000
VERSION     DBNHOX010000695.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_699205"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="AC16UZ_15130"
     CDS             complement(<1..413)
                     /locus_tag="AC16UZ_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248068.1"
                     /translation="MTRISAFLIHLGISFAIFLGLAYLVVFIWYPDFFFSTDGGWQGI
                     RIIVAVDLVLGPALTLIVFKPGKPGLKFDLTLIGLFQAACLIAGVYVVHSERPLAMVY
                     VDGQFFSMSGDDYSGVGLPIPDLRAVPGKSPKWVTV"
     gene            complement(419..1276)
                     /gene="nadC"
                     /locus_tag="AC16UZ_15135"
     CDS             complement(419..1276)
                     /gene="nadC"
                     /locus_tag="AC16UZ_15135"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR6248069.1"
                     /translation="MAPDGFPHADELADAVGESVRAALAEDVGTGDITAELIAPDARA
                     TAEVITRDEGIFCGAPWVLETCAQVDAAIEVTLHCADGDRVSPGQTLFALSGPARGLL
                     TAERTMLNFVQLLSGTATATRRYVDLIAGTAARLLDTRKTVPGLRMAQKYAVHCGGGH
                     NHRIGLFDAFLIKENHLAAAGGIAPAVARARELYPDRPVEVEVESLNELDQALAGGAD
                     IALIDNFSLEDTSAAVTLAAGRIELEASGGIDEATITEIARVGVDYISLGVITKRVIP
                     LDLSMRFTS"
     gene            1363..1914
                     /gene="ampD"
                     /locus_tag="AC16UZ_15140"
     CDS             1363..1914
                     /gene="ampD"
                     /locus_tag="AC16UZ_15140"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HIR6248070.1"
                     /translation="MRVRKDHCLVGVPFRASPNCDERPDPEDVSLIVIHGISLPPGRF
                     GGDLVDGLFTNSLPDELDPGLAELRDVRVSAHLLIDRAGRITQYVPFHRRAWHAGSSG
                     WCGRPSCNDYAIGIELEGTDELDYADAQYRALIAVSRALFDRYPRLSVDAVVGHQEIA
                     PERKTDPGPAFDWCRYLGALAGP"
     gene            1911..2885
                     /locus_tag="AC16UZ_15145"
     CDS             1911..2885
                     /locus_tag="AC16UZ_15145"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR6248071.1"
                     /translation="MKRIGIDLGGTKIKGIVLAEDGRVLSTRRAATPSQSYAGTVAAI
                     VRMVAELDGDSGEARDLPVGIGTPGIWMAQRSAMKNCNSTWLNGQPLLLDLEHALGAR
                     VRIANDADCFALSEAMAGTGRGYGSVFGVILGTGVGGGFVTGGRLMSGPNGLAGEWGH
                     TPLPYLGLHEALEISVGSELDRRHEQANALESRLAVRTCYCGRPGCVETYLSGPGWRQ
                     THRELWSEDVPTETIAKRRDERSEQTFFLYTRMLARSLAQIVNIVDPAVIVLGGGLSN
                     VQALYQPVNEMLARYVFSGECDTQVKAPQLGAESGVLGAAQLWSDNGG"
     gene            2920..>4055
                     /gene="aceE"
                     /locus_tag="AC16UZ_15150"
     CDS             2920..>4055
                     /gene="aceE"
                     /locus_tag="AC16UZ_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422575.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR6248072.1"
                     /translation="MAYPRDLRDEDAAETREWLDSLEYVLENAGVDRANFLLERLSAR
                     MTRTGARLPYTITTPYRNTIPSSREAVMPGDLFMERRIRSLIRWNALAMVVRANRRPG
                     DLGGHISSFASSATLYDVGFNYFFRGPTDEHPGDLIYFQGHASPGIYARSFLEGRLSE
                     AQLDNFRREVAGEGLSSYPHHWLMPDYCQFPTVSMGLGPIQAIYQAHIMKYLDARGLL
                     PMGERKVWAFLGDGECDEPEALGALSKAGTEKLDNLIFVVNCNLQRLDGPVRGNSKII
                     QELEGNFRGAGWNVIKVVWGRLWDPLFANDTAGLMQLRMNEVVDGEYQNYKAKGGAYT
                     REHFFGAHPELLAMVERLSDDDIMRLNRGGHDPFKVYAAYHEAV"
BASE COUNT          701 a         1299 c         1365 g          690 t
ORIGIN      
        1 accgtgaccc acttgggcga cttccccgga acggcccgca ggtcgggtat gggcaatccg
       61 accccggagt agtcgtcgcc gctcatgctg aagaactgcc cgtcgacata gaccatggcc
      121 agcggtctct cggagtggac cacgtacaca ccggcgatca ggcacgctgc ctgaaacagt
      181 ccgatcaggg tcagatcgaa tttcagtccc ggcttgccgg gcttgaagac gatcagcgtc
      241 agcgcgggac cgagcaccag atcgaccgcc acgatgatgc gaatcccctg ccagccgccg
      301 tcggtgctga agaagaagtc ggggtaccag atgaaaacga ccagataggc aagccctaaa
      361 aagatcgcga agctgattcc gagatggatc agaaaggcgc tgatacgggt catgatcgtc
      421 agctcgtgaa gcgcatggac aggtcgagcg gaatgactct cttggtgata acgccgaggg
      481 agatatagtc cacgccgacg cgggcgattt ccgtgatcgt ggcctcatcg ataccgccgg
      541 aagcctcgag ctctatgcgt cctgcagcca gcgtaacggc ggcagacgta tcctcgagcg
      601 aaaaattgtc gatgagcgcg atgtccgccc cgccggcgag ggcctggtcg agctcgttta
      661 gggactccac ctccacttcc acgggccggt ccgggtagag ctcgcgggct ctggccacgg
      721 cgggcgcgat accgccggcg gcagccagat gattctcttt gatcagaaaa gcgtcgaaga
      781 gcccgatccg gtgattgtgc ccgccgccgc agtgcacggc gtacttctgc gccatgcgca
      841 gcccgggaac cgtcttgcgg gtgtcgagca gccgagccgc cgtgcccgcg atcagatcga
      901 cgtagcgccg cgtcgccgtg gccgtccctg acaacagctg aacgaaattc agcatggtcc
      961 gttcggccgt gagcagaccc ctcgcggggc cgctcagagc aaagagcgtc tgccccggcg
     1021 agacgcgatc gccgtccgcg cagtgaaggg tcacctcgat ggcggcgtcc acctgggcgc
     1081 aggtttcgag cacccagggc gccccgcaga aaatgccctc gtcccgggtg ataacctctg
     1141 cggttgcccg ggcgtcaggc gcgatcagct ccgcggtgat gtccccggtg cccacgtcct
     1201 cggcaagagc cgcgcgcacg ctttcgccca cggcgtctgc cagctcgtcg gcgtgtggga
     1261 acccgtcggg cgccatcggg ctgcctccga tatccgatag ttgtgcgcgg gccattctac
     1321 ttcaatagaa tctgcttcag tgccggtcac ggatcacagg ttttgcgcgt caggaaagac
     1381 cattgcctcg tcggggttcc gttccgggcc tcgcccaact gcgacgagcg tcccgatccc
     1441 gaggacgtct cgctgatcgt gatccacggg atatccctgc cgccgggtcg tttcggcggc
     1501 gatctcgtcg acggcctctt caccaactcc ctgcccgatg agctggaccc cgggctggca
     1561 gagcttcgtg acgtgcgcgt ttcggcccac ctgctcatcg atcgcgccgg ccgaatcacc
     1621 cagtacgttc ccttccaccg gcgcgcatgg cacgcgggat catccggttg gtgcggccgg
     1681 ccctcgtgca acgactacgc catcggcatc gagctcgaag ggaccgatga gctggactat
     1741 gccgacgcgc agtaccgggc gcttattgcg gtgagccggg ccctgttcga tcgctacccg
     1801 cggctgtccg tggacgccgt tgttgggcac caggaaatcg cgccggagcg caagacggat
     1861 ccgggccccg ccttcgactg gtgccgttac ctcggcgcgc tggcgggccc gtgaaacgca
     1921 tcggcatcga cctgggcggc accaagatca agggcatcgt gctggcggag gacggccggg
     1981 tgctgtcgac gcgacgggcg gcgacgccgt cgcaaagcta cgccggcacc gttgcggcca
     2041 tcgtgcgcat ggttgccgag ctcgacggcg actccggcga ggcgcgtgat ctgcccgtgg
     2101 gcatcggcac ccccggcatc tggatggcgc agcggagcgc gatgaagaac tgcaactcca
     2161 cctggctcaa cgggcaaccg ctgctgctcg atctggagca tgcgctcggg gcccgcgtac
     2221 gcatcgccaa cgacgccgac tgttttgctc tctcggaagc catggccgga acgggccggg
     2281 gatatgggag cgtcttcggc gtcatcctgg gcaccggcgt gggtggcggc ttcgtgaccg
     2341 ggggacggct gatgagcggg ccgaatgggc tggccggcga gtgggggcac acgccgctgc
     2401 cttatctggg cctgcacgaa gcgctggaaa tctcggtggg aagcgaactg gatcgccggc
     2461 acgagcaggc gaatgcgcta gaatctcggc tggcggtcag aacctgctac tgcggacggc
     2521 cgggctgcgt ggaaacctac ctcagcggcc ccgggtggcg gcagacgcac cgcgagcttt
     2581 ggagcgagga cgtgcccacg gagacgattg ccaagcgtcg cgacgagcgc tcggagcaga
     2641 cgtttttcct gtacacccga atgctcgcgc gcagcctggc tcagatcgtg aacatcgttg
     2701 acccggcggt gatcgtgctg ggcggtgggt tgtcgaacgt gcaggcgctg tatcagccgg
     2761 tcaacgagat gctcgcgcgc tacgtctttt cgggcgagtg cgacacgcag gtgaaagcgc
     2821 ctcagctggg cgcggagagc ggggtgctcg gggctgcaca gctgtggagc gataacggag
     2881 gttgaggtcc ggccggctag ccggccagcg ggaactgata tggcgtatcc aagagatctt
     2941 cgcgacgagg acgcggcgga gacccgggag tggctggatt ctctcgagta cgttctcgag
     3001 aacgcgggtg tggaccgtgc caactttctg ctggagcgtt tgtctgcccg gatgacgcgc
     3061 accggcgcgc gtttgcccta caccatcacc acgccttacc ggaacaccat tccctcgagc
     3121 cgcgaagccg tgatgccggg cgatctcttc atggaacggc gcatccgctc gctgatccgc
     3181 tggaacgccc ttgccatggt ggtgcgggcg aaccggcgtc cgggcgatct gggcgggcac
     3241 atctccagct tcgcctcgtc ggccacgctt tacgacgtgg gcttcaacta tttcttccgg
     3301 ggcccgacgg acgagcaccc gggcgacctg atctactttc aggggcacgc ctcgccgggc
     3361 atatacgccc ggtcgtttct cgagggccgc ctgagcgagg cccagctgga caactttcgc
     3421 cgggaagtgg caggggaggg gctctcatcc tatccgcacc actggctgat gcccgactac
     3481 tgtcagtttc ccacggtctc catggggctg gggccgatcc aggccatcta ccaggcgcac
     3541 atcatgaagt acctggatgc ccgggggctt ctgcccatgg gcgagcgcaa ggtgtgggcc
     3601 tttctcggcg acggcgagtg cgacgagccc gaagcgctcg gcgccctgtc caaggccggc
     3661 accgagaagc tcgacaatct gatcttcgtc gtcaactgca acctgcagcg gctggacggt
     3721 cctgtgcggg gcaacagcaa gatcatccag gagctggaag gtaacttccg cggcgcgggc
     3781 tggaacgtga tcaaggtggt gtggggtcgg ctctgggatc cgctgttcgc caacgacacc
     3841 gcgggcctga tgcagctgcg catgaacgag gtggtggacg gcgagtacca gaattacaaa
     3901 gccaagggcg gggcctacac ccgcgaacac ttcttcggcg cgcatccgga gctcctggcc
     3961 atggtcgagc gcctctcgga cgatgacatc atgcggctca accggggcgg ccacgacccc
     4021 ttcaaggtgt acgcggccta tcacgaggcc gtgca
//
LOCUS       DBNHOX010000696         3369 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1663599, whole genome shotgun sequence.
ACCESSION   DBNHOX010000696 DBNHOX010000000
VERSION     DBNHOX010000696.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1663599"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="AC16UZ_15155"
     CDS             <1..925
                     /locus_tag="AC16UZ_15155"
                     /EC_number="1.6.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6248073.1"
                     /translation="SALARHGLRVLILEKSGDIGGRLATRRRPKGHWNHGAPALSPGD
                     PAFTQFLGSLESAGTAQREDGPGGLPRFQGLPDMRELLRPLVARTPLAFNTLARRLFR
                     RGASWMVETEFGDQGPFRAVVVALPAPQATDLLARSRLDGLMPPAPVTMAPVWTLLLG
                     FDEQRPPPAPADRRIVERVIPQTHRPGDRPGWCAERWVVHATARWSSDHVELDAADAG
                     ERLLRALAPSLADGDADRPDVVMTHRWRFAQTTSPMGRTHLWDPDTAVGLAGDWCLGA
                     TAEDAFTSGGALARDISKCLLTDSAKAQGHA"
     gene            918..1739
                     /gene="nei"
                     /locus_tag="AC16UZ_15160"
     CDS             918..1739
                     /gene="nei"
                     /locus_tag="AC16UZ_15160"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF007763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HIR6248074.1"
                     /translation="MPEGPEIRREADRIEAAVQGQRIEKVFFGLDRLSVWSSTLTGAT
                     VEAVGTRGKALLTTFDNGYTLYSHNQLYGKWMVRKRGTLPKTNRQLRVALHTANASAL
                     LYSASSIEVLPTDALHDYPPIARLGPDVLDTELEWRDVARRLRDPAFSGRALAALYLD
                     QHFMAGIGNYLRSEILHTAGVHPSVRPKELTTARIGELARATLAISRRSYETGGITAS
                     KAAVRRLRKQGMSRSGYRFSAFAREGLPCHRCGEPIARITANSRRLYYCRACQPR"
     gene            complement(1803..1985)
                     /locus_tag="AC16UZ_15165"
     CDS             complement(1803..1985)
                     /locus_tag="AC16UZ_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248075.1"
                     /translation="MLVVDAPPLPLDDCDRIMDCRCKYKKWSDVRQDERRSPIPNQFY
                     FDNERRGRLPGRRALD"
     gene            2179..2763
                     /locus_tag="AC16UZ_15170"
     CDS             2179..2763
                     /locus_tag="AC16UZ_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0008289 - lipid binding [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HIR6248076.1"
                     /translation="MSGAAEPITLGNLRLTGLRRAIGALCVAACCAACTGIPDGVEAV
                     TDFQLERYLGTWYEIARLDHTFERGLTRVTAEYARRSDGGISVTNRGYDAALGEWREA
                     RGKAYFLGNPATGSLKVSFFGPFYGGYHVIALDSEGYQWAMVAGPSRSYLWVLARDAV
                     VPDETFNRLVARAAELGFPTENLIFVDHSGANAH"
     gene            3118..>3369
                     /locus_tag="AC16UZ_15175"
     CDS             3118..>3369
                     /locus_tag="AC16UZ_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248077.1"
                     /translation="MTWTQILATYLVVGGTVLLFTPVRPTLARHLNLYEQRHISRLAF
                     VVYAGIVVASDLLLWPFLAAKHRPSRRRVNKPEHAVLDPE"
BASE COUNT          608 a         1131 c         1066 g          564 t
ORIGIN      
        1 atctgccctg gccaggcacg gcctgcgcgt gttgatcctg gaaaagagcg gtgacatcgg
       61 cggccgcctg gccacccgac ggcgccccaa gggccattgg aatcatggcg cgcctgcgct
      121 ctctcccggg gatccggcct tcacgcagtt ccttgggtct ctggagtctg ccgggaccgc
      181 gcagcgcgag gacggtcccg gggggctgcc ccgcttccaa ggcctgccgg acatgcgcga
      241 gctgctgcgc ccgctcgtcg cccgcacgcc actcgcgttc aacaccctcg cgcgccgcct
      301 gtttcggcgc ggagccagct ggatggtcga aaccgagttc ggggatcaag gcccttttcg
      361 agcggtggtc gtcgccctgc cggcacccca ggctaccgac ctccttgcgc gaagtcgact
      421 tgacggcttg atgccgccag cgcctgtcac aatggcgccg gtgtggacgc tgctgctcgg
      481 attcgacgag caacggccgc cgccggcccc tgccgaccgc cgtatcgtcg agcgggtgat
      541 tccacagacc catcgccccg gcgatcggcc cggatggtgc gcggagcgct gggtcgtgca
      601 cgcaacggcc cggtggtcga gcgaccacgt cgaactggat gctgcggacg ccggcgagag
      661 gctccttcgg gccctcgccc cctcgctggc tgacggagac gcggatcgac ccgacgtggt
      721 catgacacac cggtggcggt ttgcgcagac cacgtcaccc atgggcagaa cccacctgtg
      781 ggatccagac acagcggtgg gtctggcggg ggactggtgc ctgggagcga cggcggagga
      841 cgcctttacc agcggcggcg cgcttgcccg ggacatctcc aaatgcctgc tgactgacag
      901 cgccaaggcg caaggccatg cctgaaggac ctgaaattcg ccgcgaagcc gaccggatcg
      961 aggcagcggt ccaggggcag agaatcgaaa aggtcttctt cggtcttgat cgcctgtctg
     1021 tatggtccag cacgctcacc ggagcaacgg tcgaggccgt gggcacccgg ggcaaggctc
     1081 tgctgaccac gttcgacaac ggctacacgc tctactcaca caaccagttg tacggcaagt
     1141 ggatggtcag aaagcggggc acgcttccga agacaaaccg gcaactcagg gtcgcgctgc
     1201 acacggccaa cgccagcgcc ctcctttaca gcgcatcgag catcgaggtt ctgcctaccg
     1261 acgcgttgca cgattacccg cccattgccc ggctcggacc ggacgtgctc gacacggagc
     1321 tcgagtggcg ggacgtcgcc cgacgtctgc gagatcccgc attctcgggg cgcgccctgg
     1381 ccgccctgta cctggatcag cacttcatgg ccgggatcgg caactacctg cgttcggaga
     1441 ttctgcacac ggccggcgtg cacccgtcgg tcaggccgaa ggagctgacc actgcccgca
     1501 taggcgagct ggcacgcgcc accctggcca tcagccggcg gtcgtacgag acgggtggca
     1561 tcaccgcgag caaggcagcg gtcaggcgct taagaaagca aggaatgtcg cgatcgggct
     1621 accgcttcag cgctttcgcg cgggaggggc tgccatgcca tcgttgcggc gagcccattg
     1681 ctcggatcac cgcgaactcc cggcggctgt actactgccg agcatgtcag ccccgttgag
     1741 ccaacgggca ggcgtgttgc gggcggacaa gttgacgagg tgagccaaca cagcgcgccc
     1801 tgtcagtcga gcgcgcggcg tcccgggagg cggccacggc gctcattatc gaagtaaaac
     1861 tggttgggga tcggcgagcg tcgttcgtcc tgacggacat cgctccactt cttgtactta
     1921 catcggcagt ccatgatccg gtcgcaatca tcaagcggca gcggcggcgc gtcgacgacc
     1981 agcatacgat cccctgcgat tcgacgtgcc gcctcacagc agacgtcgcc ggcaggttcc
     2041 accgagaccg cggaaaaaga ggtggcagat ttgcgttcca gcatgagtca aggaagtttt
     2101 tagcttgttg gacccctaaa ctatcaacga aaccagcccc ggtctgtcgt aaaaatgcaa
     2161 ttgaattcaa cttcgaaaat gagcggcgcc gcagagccca tcacattggg aaatctcagg
     2221 ctcacaggtc tgcggcgagc gatcggcgcg ctgtgcgtcg ctgcctgctg cgcggcatgc
     2281 acaggcatcc cggacggcgt cgaggccgtg accgacttcc agctggagcg ctatctgggc
     2341 acctggtacg agatcgcacg tctggatcac accttcgaaa gggggctcac tcgagtgacg
     2401 gccgagtatg cccgccgaag cgacggtggc atcagcgtga cgaaccgcgg ctacgacgcc
     2461 gcccttggcg agtggcggga agcccggggc aaagcctact tcctcggtaa ccccgccacc
     2521 ggcagtctga aggtgtcgtt cttcgggccc ttttacggcg gctatcacgt gattgctctg
     2581 gacagtgagg gctaccaatg ggccatggtc gcgggcccga gccgttcata tctctgggtt
     2641 ctcgccagag acgccgttgt gccagacgag acgttcaacc gcctggtggc gcgcgccgcc
     2701 gagctcggct tcccaaccga gaatctcata ttcgtcgatc actccggcgc gaatgcccac
     2761 tgaaagaagg acggtaaggg cgacccgccg ggctgatgac ccaatctggc catggccatg
     2821 accggctgtt aggcatcttt ctacaaatcg ctcacaaaac ggcgttcctt cgacgccgcg
     2881 ccacgtctaa cctacttgaa tgatcggggg gccagaatac gacacaccag cgcaccggcg
     2941 ttgggatagg ggcagcgaaa gcgacggccc gatgcccacc cgccatcggc gctcacacgg
     3001 cggtcccgac aggaacccaa gcacgctgta cgacccccgc caactggctt tgctgcctga
     3061 actccagggc gatctcttcg aggagctgtc cagttccatg gtaagcgggc gatgacgatg
     3121 acctggacgc agatcctcgc aacctacctc gtcgtcggcg gcaccgttct cctattcacc
     3181 ccagtccggc ccacgctcgc gcgtcatctg aacctatacg agcagcgcca catttcccgc
     3241 ctggcgttcg tcgtctatgc cggtatcgtc gtggccagcg acctgctcct ctggccgttt
     3301 ctcgccgcca aacatcgccc ctcgcgccgg cgggtcaaca agccggagca cgcggtcctg
     3361 gacccggag
//
LOCUS       DBNHOX010000697         5174 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1406858, whole genome shotgun sequence.
ACCESSION   DBNHOX010000697 DBNHOX010000000
VERSION     DBNHOX010000697.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1406858"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="AC16UZ_15180"
     CDS             complement(<1..1314)
                     /locus_tag="AC16UZ_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248078.1"
                     /translation="MTQNTSTPVDAALQASTRAPRDTARLDALGGFLMREFETYERDR
                     QPAELRWAKHARQFLGQYDPDVAATLDPSRSKAYPKLTRVKCVSMLSRLMNLLFQSSE
                     KNWSIQPSHVPSLDDTDLQMLLTNLQLSQDPNAPLDDAMIELAIMEFAKERSNNLERE
                     IADQLQEIGGSRQVDYVALCRKVMMSAIIYGMGVLKGPFIRSTQLRTWERDATGAPVA
                     KSYMAMRPQFEFVPIWDYYPDMSAKYLHQMDGQFQRIVMAHHQVRELADRDDFFGDVI
                     KQYLRDNPKGNYRQRTFESELKAMGVHNNVNDQSGRKYELLVWDGFIKGRDLQAAGVE
                     VKEEQLTDRIEAVVWLIDGKVIKADMNPWVRLGIDQKVNSYHHFIFEENESTITGNGL
                     PNIMRDSQMAICSATRMVMDNASVVCGQNLEVNLDLVDASQDLASV"
     gene            complement(1356..2771)
                     /locus_tag="AC16UZ_15185"
     CDS             complement(1356..2771)
                     /locus_tag="AC16UZ_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248079.1"
                     /translation="MTARINYTPPPTVGAFIRDYKASELFFDWIIGPVGSGKTTGIFF
                     KLVYMAGLQAPSPLDGIRRSRAVIVRNTMPQLRDTTLASWNYWFKDGVAGTWRATNSS
                     FLLKFDDVECEVLFRPLDTPDDVQRVLSLEVTFAILDEFVQIDPAIVEALSARCGRYP
                     SIIDGGATNWGMWGSSNPGTEDSWWHEKLEENLPGNWTYFKQPSGFAPHAENLDNLPG
                     KRAYYTSLAEGKSEHWVKQFIEVCWGYSLDGTPVLKSFNAEWHVARRPLLFNPHLPLV
                     GGFDPGMNCAMVFGQQDHSGRLLVLSALVAEGYGAKRFIAEKLKPHLAARYPGLQDFV
                     VSPDPAAFTRSQTDERTVVAEFKAQHFNVQVPDMNNQLTKRIEAMEHYTSRLTPAGPA
                     LQIDPLYAKPVVTALQTGWRYKVKKEGEVNDTPEKNKHSHPADAFGYLCRWHAQADPD
                     YTRANRSMRTPVSRPPNYHAR"
     gene            complement(2768..3256)
                     /locus_tag="AC16UZ_15190"
     CDS             complement(2768..3256)
                     /locus_tag="AC16UZ_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248080.1"
                     /translation="MSAAFPAATSILSTLRPDDPSELGFPPMLPVELALRVAPVPDIC
                     AAYGLTREAFEVLVAHPEFVRAYQAAVEALKQDGMSFKMKAKLQAEEMLQQSWNLIHD
                     ADTPPAVKATLIKDTVRWAGYEPKGTTGAGEGGPGLHISINFVNPAPAETGAITIDAP
                     AE"
     gene            complement(3260..3442)
                     /locus_tag="AC16UZ_15195"
     CDS             complement(3260..3442)
                     /locus_tag="AC16UZ_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248081.1"
                     /translation="MEEFGANCQTPDGLMYYCRTCNNAKQREYREKNPAKAKANRERY
                     LENVRRRNREARRAKE"
     gene            3795..3968
                     /locus_tag="AC16UZ_15200"
     CDS             3795..3968
                     /locus_tag="AC16UZ_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248082.1"
                     /translation="MNTNELTTRTALARDNASVFGTRRAFAIELERLARRGVRGERAF
                     GLAALHLFGRWVR"
     gene            4070..4339
                     /locus_tag="AC16UZ_15205"
     CDS             4070..4339
                     /locus_tag="AC16UZ_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248083.1"
                     /translation="MSKMSELHAELQELATRVGIDGLADTIELCAMTDELLRMWDCGC
                     LHHTDEAIELVTSFACEEYGFDLPDELRSHLWLHALRTRLEVTYH"
     gene            4529..5116
                     /locus_tag="AC16UZ_15210"
     CDS             4529..5116
                     /locus_tag="AC16UZ_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248084.1"
                     /translation="MKKQIAEVAATVENVAAAIREMAKCRVKNDEVHARLGKLMVRLF
                     IAHCGSDEDFFGTFGDGKKIGGELRKTVVDGIPTNKDGKFKDPVYAKGVLDVLKVRLA
                     EARRLRKDGGMAHEDDNNLQASAKRYAKAKAAAPKTPEGESATVVISADASMADIAEA
                     VSVWVAKHGNAGAAGLAAALKDCLPITPRRMRKAS"
BASE COUNT          869 a         1576 c         1719 g         1010 t
ORIGIN      
        1 caccgaggcg aggtcttgcg aggcgtcgac gaggtcgagg ttgacctcca gattctgccc
       61 acacacaaca gaggcgttgt ccatcaccat gcgggtggcg ctgcagatgg ccatctggct
      121 gtcgcgcatg atgttcggca ggccgttgcc ggtgatcgtc gactcgttct cctcgaagat
      181 gaagtggtgg tacgagttga ccttctggtc gatgccaaga cgcacccacg ggttcatgtc
      241 ggccttgatg accttgccgt cgatcagcca cacgacggcc tcgatgcggt cggtgagctg
      301 ctcctccttc acctcgacgc cggcggcctg caggtcgcgg cccttgatga acccgtccca
      361 gaccagcagc tcgtacttgc gcccggactg gtcgttgacg ttgttgtgca cacccatcgc
      421 cttcagctcg gactcgaagg tgcgctgccg gtagttgccc ttcgggttgt cgcgcaggta
      481 ctgcttgatg acatccccga aaaagtcgtc ccggtcggcc agctcgcgga cctgatggtg
      541 cgccatgacg atacgctgga actggccgtc catctggtgc aggtacttcg cgctcatgtc
      601 cgggtaatag tcccagatcg gcacgaactc gaactgcggg cgcatcgcca tgtacgactt
      661 ggccactggt gcaccggtgg cgtcccgctc ccacgtacgc agctgggtgg agcggatgaa
      721 cgggcccttg agcacgccca tgccgtagat gatcgcgctc atcatgacct tgcggcatag
      781 cgcgacgtag tcgacctgcc ggctgccgcc aatctcctgc agctggtcgg caatctcccg
      841 ctccagattg ttgctgcgct ccttggcgaa ctccatgatg gccagctcga tcatcgcgtc
      901 gtcgagcggc gcgttcggat cctgcgagag ctgcaggttg gtcagcagca tctgcaggtc
      961 ggtgtcgtcg aggctcggca cgtgcgaggg ctggatggac cagttcttct ccgaggactg
     1021 gaacagcagg ttcatcaggc gcgagagcat cgacacgcat ttcacacgtg tgagcttcgg
     1081 gtacgccttg gagcggctcg ggtcgagcgt cgccgccacg tcagggtcgt actggccgag
     1141 gaactggcgg gcgtgcttgg cccagcgcag ctcggcgggc tggcggtcgc gttcataggt
     1201 ctcgaactcc cgcatgagga acccaccgag ggcgtcaagg cgggcggtgt cgcgcggggc
     1261 gcgcgtcgag gcttggaggg cggcgtccac cggggtggag gtgttctggg tcatgtcggg
     1321 ctccgaaggg ggcggttcga ggcgatcatc ttatctcacc tcgcatggta gttcggcggc
     1381 cggctcaccg gcgtgcgcat cgagcggttg gcccgggtgt agtccggatc cgcctgcgca
     1441 tgccaccggc acaggtagcc gaacgcatcc gccgggtggc tgtgcttgtt cttctccggg
     1501 gtgtcgttga cctcgccttc cttcttcacc ttgtaccgcc accccgtctg caacgccgtg
     1561 acgaccggct tcgcatagag ggggtcgatc tgcagcgcgg ggcccgccgg cgtgaggcgg
     1621 gaggtgtagt gctccatcgc ctcgatgcgc ttggtgagct ggttgttcat gtccggcacc
     1681 tgcacattga agtgctgggc cttgaactcc gccaccacgg tgcgctcgtc ggtctgcgag
     1741 cgcgtgaacg ctgccgggtc ggggctgacg acgaagtcct gcagcccggg gtagcgcgcg
     1801 gcgaggtggg gcttcagctt ctcggcgatg aaccgcttgg cgccgtagcc ctccgcgacg
     1861 agggccgaga gcaccagcag gcggccactg tggtcctgct ggccgaagac catcgcgcag
     1921 ttcatccccg ggtcgaagcc ccccaccagc ggcaggtgcg ggttgaacag cagcggccgg
     1981 cgcgcgacgt gccactctgc gttgaacgac ttgagcaccg gcgtgccgtc gaggctgtag
     2041 ccccagcaca cctcgatgaa ctgcttgacc cagtgctcgg acttgccctc ggccaagctg
     2101 gtgtagtacg cccgcttgcc cggcaggttg tccaagttct ccgcgtgcgg cgcgaagccc
     2161 gagggctgct tgaagtacgt ccagttcccc ggcaggttct cttcgagctt ctcgtgccac
     2221 cacgagtcct cggtgcccgg gttgcttgac ccccacatgc cccagttggt cgccccgccg
     2281 tcgatgatcg acgggtagcg gccacaacgg gcggagaggg cttccacgat ggccgggtcg
     2341 atctgcacga actcgtcgag gatggcgaag gtcacctcca gcgagagcac gcgctgcacg
     2401 tcgtcggggg tgtccagcgg gcggaacagc acctcgcact ccacgtcgtc gaacttgagc
     2461 aggaacgagg agttggtcgc gcgccacgtg ccggccacgc cgtccttgaa ccaatagttc
     2521 cagctggcga gcgtggtgtc ccggagctgc ggcatggtgt tccgcacgat cactgcgcgc
     2581 gagcggcgga tgccgtcgag cggggagggg gcctgcaggc ccgccatgta gacgagcttg
     2641 aagaagatcc cggtggtctt gcccgagccg accggcccga tgatccagtc gaagaacagc
     2701 tccgacgcct tgtagtcgcg gatgaacgca ccgacggtgg gcggcggcgt gtagttgatg
     2761 cgagcggtca ttcagcgggg gcgtcgatag tgatggcgcc ggtctcggcg ggcgccgggt
     2821 tgacgaagtt gatgctgatg tgcagcccgg ggccaccctc gccggcgcca gtggtgccct
     2881 tgggctcata gcccgcccag cggaccgtgt ccttgatgag cgtcgcttta accgccggcg
     2941 gggtgtccgc gtcatggatc aggttccacg actgctgcag catctcctca gcctgcagct
     3001 tggccttcat cttgaagctc atcccgtcct gcttgagcgc ctccacggcg gcctgatacg
     3061 cgcggacgaa ctcggggtgg gcgacgagca cctcgaatgc ctcacgcgtg aggccgtagg
     3121 ccgcgcagat gtccggcacg ggcgccacgc gcagtgccag ctccaccggc agcatgggcg
     3181 ggaagcccag ctcggacggg tcgtccgggc ggagcgtgga caggatcgaa gtggcggctg
     3241 gaaaggcggc gctcatggtt tactccttcg cgcggcgagc ctcccggtta cgccggcgca
     3301 cattctccaa gtaccgctcg cggttggcct tggccttggc cggattcttc tcgcggtact
     3361 cgcgctgctt ggcattgttg cacgtgcggc aatagtacat cagcccgtcc ggggtctggc
     3421 aattggcgcc gaactcttcc acaggcttga cctcgtggca gcaggagcat cgcttggtgg
     3481 ctgggggtct gtattccatc ttcgtacgtg ggtgctgtaa tggataaaag tatagcaggg
     3541 aacgaagagg tgggcggggt ttttgacttt tataggtttt gtaccttcta tacctacttt
     3601 gcttttccaa aaattttcag attttatatg cgatacaggt atatgtatag gtaggggtgg
     3661 accatcgaac ccccttgggg ggccctccag ataaaaagaa ttcttttctc ctcaagggct
     3721 tgcgtcgcgt accaagatcc ggagagtgtg ggttgtggca gggatggccc tgcccggact
     3781 tcaggagaaa gaaaatgaac acgaacgaac tcaccacccg caccgccctt gcccgtgaca
     3841 acgccagcgt gttcggcaca cgtcgcgcct ttgctatcga gctggagcgc ctcgctcgtc
     3901 gcggcgtgcg cggtgagcgg gccttcggcc tcgcggcctt gcacctcttc ggtcgctggg
     3961 tgcggtaatg cggccggcgc cccgcgaggg gcgcggttac atccgcgcct gcatcctcgg
     4021 gctggcgttc gccggcctga tcttcctctt cctttgattg gagaacatca tgagcaaaat
     4081 gtctgaactg cacgccgagc tgcaagagct ggcaacccgc gtcggtatcg acggcctcgc
     4141 cgacacgata gagctgtgcg ccatgaccga cgagctgctg cgcatgtggg actgcggctg
     4201 cctgcaccac acggacgaag cgattgagct ggtgacctcg ttcgcatgcg aggagtacgg
     4261 gtttgaccta cccgacgaac tgcgcagcca cctctggctg cacgcgctgc gcacgcggct
     4321 tgaagttacg taccactgac ctgagcgagg gggcttcggc cccctcctct ttttatcacc
     4381 ctcctccctc gcgtgacagt agtagtccgc accatccgag ccgttcgggt tactgagtaa
     4441 cgcgcgcttg catcgcgtac caagatcagg atagttcggg gtgtggaggg aatggtcccg
     4501 ccacgcaatg aatcgaaagg tgaatcgaat gaaaaagcaa atcgcagaag tcgccgcaac
     4561 tgtcgaaaac gtcgccgctg caatccgtga gatggctaaa tgccgcgtca aaaacgacga
     4621 ggtgcatgcg cggcttggca agcttatggt gcgcttgttc attgcgcatt gcggatcaga
     4681 cgaggatttc ttcggcacgt tcggcgatgg aaagaaaatc ggcggcgagc tacggaaaac
     4741 tgttgttgat ggtatcccca cgaacaaaga cgggaagttc aaagatcccg tatacgcgaa
     4801 gggggttctg gatgtgctaa aggtgcgctt ggctgaggcg cgccgcctgc gcaaggatgg
     4861 cgggatggcg cacgaagacg acaacaatct gcaggcgtct gcaaagcggt acgcgaaagc
     4921 gaaagccgct gcgccgaaga cgccagaggg cgaatccgct accgtcgtga tatcggcaga
     4981 cgccagcatg gccgatatcg ctgaggcggt atctgtttgg gtcgccaagc atggcaatgc
     5041 aggcgcggcc gggttggccg cggcgttgaa ggattgcttg ccgatcacgc cgcgccgcat
     5101 gcgcaaggca tcgtaaccac ggggcgccct tcggggcgcc ctttttttcg tccgcgtttt
     5161 ggggcgccct tcgg
//
LOCUS       DBNHOX010000698         2173 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1084744, whole genome shotgun sequence.
ACCESSION   DBNHOX010000698 DBNHOX010000000
VERSION     DBNHOX010000698.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1084744"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1684
                     /locus_tag="AC16UZ_15215"
     CDS             <1..1684
                     /locus_tag="AC16UZ_15215"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GcvT family protein"
                     /protein_id="HIR6248085.1"
                     /translation="DQGIYLKPDAGRLLVGCFESAAKPLAPESLPDEFAFDELPFDLD
                     HFAPYLQNAVDRLPALGNTGIRTWFNGPESFTPDGRYIIGPLPEIRGLFAACGMNSIG
                     IQSAGGIGQVMARWVTDGRPPMDLWEVDARRFMPFQNETDYLVERTSEALGLLYAMHW
                     PYRQHESARPIRQSPLHERLAAAGACFGELAGWERANWYARPGQAPRYEYSYGRQNWF
                     PNAAQEHRAAREAVALFDQSSFAKYEVSGPDACRLLNRLGTSDIDVAIGRVVYCQWLN
                     ERGGIEADVTVTRLGPETFWVVSAAPCQRRDLAWLAEHSAGFQADVKDVTDQHAVIGV
                     MGPDSRRLLTSLADADFGHDAFPFATSQPIAIANTALRATRITYVGALGWELYVPRAE
                     AVTVYDAIAGAGPVHAGYHAMDSLRLEKAYRHWGHDITDEDTPIEAGLSFTAAWDKPE
                     GFIGREALLEQRADGVTKRLALFVLNDPDPLLYHDEPIWWNGRPVGRITSGAYGHTLG
                     RSMGFGYVAADAPFPRQALREGRFQIEIEDTLVDAACHLRAPFDPDNREILR"
     gene            complement(1691..>2173)
                     /locus_tag="AC16UZ_15220"
     CDS             complement(1691..>2173)
                     /locus_tag="AC16UZ_15220"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR6248086.1"
                     /translation="LAAGSELEVGDPAPGFTLEGSDGRTYSLSDYVGRQAVVIAWFPK
                     AYTRGCTIECKSLAENGHLIRRFDVSYFMASVDPIEDNIGFAEQQKADFPLLSDPTKE
                     TARAYGVLSFAGFAKRHTIYIAKDGTIAAIDTDVRPATSAEDIATRLGELGVAAAPES
                     "
BASE COUNT          371 a          747 c          703 g          352 t
ORIGIN      
        1 ggaccagggc atctatctga aacccgatgc agggcggctg ctggtgggct gcttcgagtc
       61 cgccgccaag ccccttgcgc ccgagtctct accggacgaa ttcgccttcg acgagctgcc
      121 cttcgacctc gaccacttcg cgccgtacct gcagaacgcc gtggaccgtc tgccggcatt
      181 gggcaacacg ggcatacgca cctggttcaa cggcccggag agcttcacgc cggacggccg
      241 ctacatcatc ggccccctcc ccgagatccg gggcctgttc gcagcctgcg ggatgaactc
      301 catcgggatc cagtccgccg gcggcatcgg ccaggtcatg gcacgctggg tcaccgacgg
      361 ccgccctccc atggatcttt gggaggtgga tgcacgccgt ttcatgcctt ttcagaacga
      421 gaccgactac ctcgtcgagc gaacgtccga ggctctgggc ctgctctacg ccatgcattg
      481 gccgtatcgc cagcacgaaa gcgcaagacc catccgccag tcgccattgc acgagcggct
      541 ggccgccgcc ggtgcctgtt tcggcgagct ggccgggtgg gaacgggcca actggtacgc
      601 ccgaccgggc caggcgcccc gctacgagta cagctacggc aggcagaact ggttcccaaa
      661 tgctgcccag gagcatcgcg cggccaggga agccgtcgcc cttttcgatc agtcgagctt
      721 tgccaagtac gaggtttccg ggccggacgc gtgccgcctg ctcaaccgcc tcggcacgtc
      781 cgacatcgac gtcgccatcg gccgggtcgt gtactgccag tggctcaacg agcggggcgg
      841 gatcgaagcg gacgtgaccg tgacccggct tggtcctgaa acgttctggg tcgtatccgc
      901 tgcgccctgc cagcgccggg acctggcatg gctagccgag cacagcgccg gtttccaggc
      961 cgacgtgaaa gacgttaccg accagcacgc cgtcatcggc gtcatgggac ctgacagccg
     1021 cagactgctg acatcgctcg cggacgcgga cttcggccac gacgcctttc ccttcgccac
     1081 ctcacagcca atcgccatcg caaacaccgc cctgcgcgcc acgcgcatca cctacgtcgg
     1141 cgccctgggc tgggagctct acgtgccccg ggccgaagcc gtcacggtgt acgacgccat
     1201 cgccggggca ggccccgttc acgccggcta tcacgccatg gactcgctgc ggctggagaa
     1261 agcgtaccgg cactggggtc acgacatcac cgacgaggac acgcccatcg aggcgggact
     1321 cagcttcacc gccgcctggg acaagccgga ggggttcatc ggccgcgaag cgctgctcga
     1381 gcagcgggcg gacggcgtga cgaagcggct cgccctgttc gttctgaacg accccgaccc
     1441 cctgctgtac cacgacgaac cgatctggtg gaacggcagg cccgtgggcc gtatcacatc
     1501 aggcgcctac ggccacacgc tcggacggtc gatgggtttc ggctacgtcg cagccgatgc
     1561 gcccttcccc cgccaggcgc tgagggaagg acgatttcag atcgagatcg aggacacgct
     1621 ggtggacgca gcctgccacc tcagggcgcc cttcgacccg gacaaccggg aaatactgcg
     1681 ctgagcggga ctagctctcg ggcgcggcgg ccaccccaag ctcacccagc cgggtcgcga
     1741 tgtcctcggc agacgtggcc ggacggacgt cggtatcgat ggcggcgatg gtgccgtcct
     1801 tcgcgatgta tatggtgtga cgcttcgcga acccggcgaa gctgaggacg ccgtaggccc
     1861 gggccgtttc cttggtggga tcgcttaaga gcggaaagtc ggccttctgc tgctcggcaa
     1921 aaccgatgtt gtcctcgatg ggatcaacgc tggccatgaa gtagctgacg tcgaaccggc
     1981 gtatgaggtg accgttctcg gccagcgact tgcactcgat ggtgcagccg cgggtatagg
     2041 ctttggggaa ccaggcgatt acgacggcct gtcggccgac gtagtcggac aacgaatagg
     2101 tccgaccgtc gctaccctcc agcgtgaatc cgggcgcggg gtcacccact tcgagctcgc
     2161 tgccggcggc cag
//
LOCUS       DBNHOX010000699         1938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_957130, whole genome shotgun sequence.
ACCESSION   DBNHOX010000699 DBNHOX010000000
VERSION     DBNHOX010000699.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_957130"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="AC16UZ_15225"
     CDS             <1..432
                     /locus_tag="AC16UZ_15225"
                     /inference="COORDINATES: protein motif:HMM:NF025426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HIR6248087.1"
                     /translation="GYVDFGELSGTFGEPTVQISIGASLLNLVGSLSAEEDPETAELF
                     KKLNGVRVNVFETESMAEGAVDLIKGISSQLSSSGWESVVTVNSADEQVRVFMKMNAE
                     TVDGITVMALEENEAVFVNVIGNINPAEIQRVMKNFDVEID"
     gene            432..995
                     /locus_tag="AC16UZ_15230"
     CDS             432..995
                     /locus_tag="AC16UZ_15230"
                     /inference="COORDINATES: protein motif:HMM:NF025426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HIR6248088.1"
                     /translation="MRLNGIRVPMLVLLCAWSLAACGLTAPRGSEGFADLDSLGLADT
                     DRVMSISIGPALLRFAASHIDDDPETEALLRSLDGVRIRIYEIDGEARRVASRMEGMS
                     RKLQDGGWEQVMLVRSAGEQAHMLMRVADQRICGMTVLVSDGESEAVVINLMGDLQPG
                     QFTDVMAALDVDAGGVQDLQPVDARES"
     gene            complement(1019..1300)
                     /locus_tag="AC16UZ_15235"
     CDS             complement(1019..1300)
                     /locus_tag="AC16UZ_15235"
                     /inference="COORDINATES: protein motif:HMM:NF025392.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HIR6248089.1"
                     /translation="MQTFVILRRSAWESEEDLEAAAELSRQVADEDLDGRVKWIRSYV
                     LEEEDGTLGTVCIYQAEDEEAVLEHAQRAELPADEVIPVANTVIIRDDP"
     gene            1430..1852
                     /locus_tag="AC16UZ_15240"
     CDS             1430..1852
                     /locus_tag="AC16UZ_15240"
                     /inference="COORDINATES: protein motif:HMM:NF014678.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HIR6248090.1"
                     /translation="MRTLFLTMAVFLCTSCVAGEPYVVLKGQRFTVELAETSEKQALG
                     LMFRDSLADDHGMLFLFPAEARRSFWMKNTRIPLDILYFDAQLKLVSVAAGAQPCRTP
                     SCPPYPSAGPARYVLELNAGKAAELGASPGDELELHID"
BASE COUNT          342 a          584 c          655 g          357 t
ORIGIN      
        1 ggctacgtgg actttggcga gctgagcggc acctttgggg agccgaccgt gcagatttcc
       61 atcggcgcct cgctgctgaa cctggtcggc tcgctgagcg ccgaagagga tccggagacg
      121 gccgagctgt tcaagaagct gaacggcgtg cgcgtcaacg tcttcgagac ggaatccatg
      181 gccgagggtg cggttgacct gatcaagggc atctcctcgc agctcagctc cagtggctgg
      241 gagtcggtgg tgaccgtcaa ctcggccgac gagcaggtcc gcgtgttcat gaaaatgaac
      301 gcggagacgg tcgacggcat caccgtgatg gcgctcgagg aaaacgaggc cgtgttcgtc
      361 aacgtcatcg gcaacatcaa cccggcggaa atccagcgcg tgatgaagaa ctttgacgtg
      421 gagatcgact gatgaggctg aatggaatcc gcgtcccgat gctggtgttg ctctgcgcgt
      481 ggtcgctggc tgcgtgcggg ctcacggcgc cccgcggcag tgagggtttc gcggacctcg
      541 attcactggg cctggcggac accgaccggg taatgtcgat ttccatcggc ccggcgctgc
      601 tccgtttcgc ggccagccac atcgatgatg accccgagac agaggctttg ctgcgcagcc
      661 tggacggcgt gcgcatccgc atctacgaaa tcgacggcga agccaggcgg gtggcgtcgc
      721 gaatggaggg gatgagccgg aagctgcagg acgggggctg ggagcaggtg atgctggtgc
      781 ggtcagccgg cgagcaggcg cacatgttga tgagggtcgc agatcagcgc atctgcggca
      841 tgacggtgct cgtttcagac ggcgaatcgg aggccgtggt catcaacctc atgggtgacc
      901 tgcagccagg gcagttcacc gatgtgatgg cggcgctgga cgtggatgcc ggcggcgtgc
      961 aggacctgca gcccgtggac gcgcgggaaa gctgagcacc cggccgggta atccggcctc
     1021 aggggtcgtc gcggatgatg accgtgttgg cgaccggaat cacctcgtcc gccggcagtt
     1081 cggcccgctg ggcatgttcc aggacggctt cctcgtcttc cgcctggtat atgcaaaccg
     1141 tgccgagcgt gccgtcttcc tcttcgagca cgtaggagcg gatccatttc acgcggccgt
     1201 ccaggtcctc gtcggccacc tgcctggaaa gctccgctgc ggcttccagg tcttcctcgg
     1261 actcccatgc gctgcggcgc agaattacaa aggtttgcat gatgtaaggg ctccttgaac
     1321 ttggcctgtc agcggcctct cgacttatca tagaacagaa cgggcttttt gcagacgcgc
     1381 gccaaaagtc atttgaaaaa taaacgcacc cccggcagac tccgcaatca tgcgcacgct
     1441 gtttctgacg atggccgttt tcctctgcac gtcctgcgtg gccggcgagc cctacgtcgt
     1501 gctcaagggc cagcgcttca ccgtggagtt ggcggaaacc agcgaaaagc aggctttggg
     1561 cctgatgttc agggacagct tggccgacga ccacggaatg cttttcctgt ttcccgcgga
     1621 agcgaggcgt tccttctgga tgaagaacac ccgcattccg ctggacatcc tctacttcga
     1681 tgcgcagctc aaactggtca gcgtcgccgc cggcgcgcag ccgtgccgga cgccgagctg
     1741 cccgccttac cccagcgccg ggccggcccg gtacgtgctg gaactcaatg cgggcaaggc
     1801 ggccgagctg ggcgcgagtc ccggtgatga gctggagttg cacatcgatt gacgtcatcg
     1861 cggtgcggcg gctcgatggt cacctggctc caaaggcgca gccccggccg gtagggcacc
     1921 agccggtccg cggccagg
//
LOCUS       DBNHOX010000700         1519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1663836, whole genome shotgun sequence.
ACCESSION   DBNHOX010000700 DBNHOX010000000
VERSION     DBNHOX010000700.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1663836"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="AC16UZ_15245"
     CDS             complement(<1..1477)
                     /locus_tag="AC16UZ_15245"
                     /inference="COORDINATES: protein motif:HMM:NF015148.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HIR6248091.1"
                     /translation="MSYASWSFFQRIGLARTPMTILENNCMQSTASAAGDSTGTTLVS
                     AFSAYIMINGTSLPLGEMFAWVFVLAVLGVTMAIPMKRQMVNVEQLRFPTGIAAAVTL
                     RTLYSEGARAARSARALGTGGVLAAISQFWMDGMHLLGAGFSKYSLGAAFGKFNVATF
                     GPAWMGRTVLFQWDPIFIAGGALMGIRAGASMLLGGTLCWVVFVPILQANGTITGEGY
                     REIVQWPLWGGVSCMVTSGLLAFFLQWRSIVKAVSSVGKMFGGKPDAGTDVLASVEAP
                     MSWFFLGQFVALVAIVWLAKSSFDMPVWQSVLAVLLSFFLALVACRVTGETDTTPTGA
                     MGKVTQLTFGALSPGNMNINLMSANITSSAAISAADLLTDLKSGYLLGANPRKQFLAQ
                     FAGIFIGTLATVLAFRIIVPDASVLGSDQFPAPAAQTWRVVALALGQGLQALEPLKVW
                     LMAAGGALGIVLTLAPRWLPRYQEWIPSPAAFGLAWTFHWYY"
BASE COUNT          294 a          509 c          503 g          213 t
ORIGIN      
        1 cgtagtacca gtggaaagtc caggcgagtc caaacgcggc gggcgagggg atccactcct
       61 ggtacctcgg caaccagcgc ggcgccaggg tcagcacgat tcccagcgcg ccgccggccg
      121 ccatcagcca gaccttcagc ggttcgagcg cctgcagccc ctgccccagc gcaagggcga
      181 cgactcgcca ggtctgcgcg gcgggagccg gaaactggtc cgaaccgagt accgaggcgt
      241 ccgggacgat gatcctgaag gcgagcacgg tggcaagcgt gccgatgaag atgcccgcga
      301 actgggcgag gaactgcttg cgcgggttgg cgcccagcag gtagccgctc ttcaggtccg
      361 tcagcaggtc ggccgccgag atcgcggcgc tggaggtgat gttggcgctc atcaggttga
      421 tgttcatgtt gccggggctg agcgcaccga aggtcagctg ggtcaccttg cccatggccc
      481 cggttggagt cgtgtcggtc tcgccggtca cacggcaggc caccagcgcg aggaagaatg
      541 acaggaggac ggcgagaacg ctctgccaga cgggcatgtc gaaactggac ttggccagcc
      601 acacgatggc caccagtgcg acgaactgcc cgaggaagaa ccacgacatc ggcgcctcga
      661 cgctggccag cacatcggtc ccggcatccg gcttcccgcc gaacatcttg cccaccgacg
      721 ataccgcttt cacgatgctg cgccactgca ggaagaaggc cagcaggccc gaggtcacca
      781 tgcagctgac cccgccccac agcggccact gcacgatttc gcgatagccc tcgcccgtga
      841 tggtcccgtt ggcctgcagg atggggacga agaccaccca gcacaaggtg ccgccgagca
      901 gcatgctggc acccgcacgg atgcccatca gtgcgccgcc ggcaatgaaa atggggtccc
      961 actggaacaa tacggtccgt cccatccagg cgggaccgaa tgtggcgacg ttgaacttgc
     1021 caaaggcggc gcccaggctg tacttggaga acccggcgcc cagcaggtgc atgccgtcca
     1081 tccagaactg gctgatggca gccagcacgc cgccggtgcc cagcgcacgc gccgagcgcg
     1141 ccgcgcgggc gccctcggag tacaaggtac gcagcgtcac ggccgcggcg atacccgtgg
     1201 ggaagcgcag ctgctcgacg ttgaccatct gccgcttcat cgggatcgcc atggtcacgc
     1261 ccagcaccgc cagtacgaac acccaggcga acatctcgcc caggggcagg gaggtgccgt
     1321 tgatcatgat gtaggccgag aaggctgaca ccagcgtcgt gccggtcgaa tctcccgccg
     1381 cgctggcggt ggactgcatg cagttgttct cgagaatcgt catcggcgtg cgcgcaagcc
     1441 cgatgcgctg gaaaaagctc caggacgcgt acgacacgat gcaggccgtg atggcgacgc
     1501 cgaatcccca tccggcctt
//
LOCUS       DBNHOX010000701         1684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_56363, whole genome shotgun sequence.
ACCESSION   DBNHOX010000701 DBNHOX010000000
VERSION     DBNHOX010000701.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_56363"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..1221
                     /locus_tag="AC16UZ_15250"
     CDS             355..1221
                     /locus_tag="AC16UZ_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024914544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal domain-containing
                     protein"
                     /protein_id="HIR6248092.1"
                     /translation="MRDLNYQLKQLCRHNRDGGYATQRNRERLLSLIADQLHALGFRG
                     MGAQSLKPKHVEALVNRWQAESLSVGTIKNRMTVLRWWAQKVNRQNVIARQNDHYGIP
                     ERVFVSTDSKARQIDGAALERIKDDHVRMSLELQQAFGLRREEAIKFQPSYADQGDYL
                     VLKDSWTKGGKAREIPVRTVAQRTVLDRAHRLAGRGSLIPAERNYRQQLRVYERHTAN
                     AGLSKLHGLRHAYAQGRYEELTGWKAPAAGGPTSRSLTREQRVVDREARLVISRELGH
                     EREQITTVYLGR"
     gene            1313..>1684
                     /gene="pilL2"
                     /locus_tag="AC16UZ_15255"
     CDS             1313..>1684
                     /gene="pilL2"
                     /locus_tag="AC16UZ_15255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFGI-1 class ICE element type IV pilus protein
                     PilL2"
                     /protein_id="HIR6248093.1"
                     /translation="MKRTQAHQRHLPLVAACILGALLAPLEANAAEVTVARYSTVQPA
                     PSMAQRDPLSAPVVSVLPASVTRIGEAVETLLGPSGYRLASPFAAEAERAELLNLPLP
                     ESHRTLGPLPLRTALAILAGPA"
BASE COUNT          318 a          563 c          531 g          272 t
ORIGIN      
        1 gtcgtttcac atccactcgc accggccgcc agcacggcgt cagcgagtgc ttcctcaacc
       61 cagcgatgtc ctgtcctaat cgcctaactc aacggaggta atgcctatcg gcccatccac
      121 ttcttcaact caagcacatc gaaaaaggag gtgatgcgta ttcgaagctc ggcccgcaac
      181 gggcccgttg gttcgcaccg gaccgattcc caacagggaa gaaccggtgt tgcctgcaac
      241 caggcatctt caggttgaac gggagtggtg tcccgtgacc cgtgccggat tccggacctg
      301 gtcggctacg gcctgccggg tggtcagaaa cacttaccag agcatcgtga caccgtgaga
      361 gacctgaact accaactcaa acagctctgt cgccataacc gcgacggagg ctatgcgacc
      421 cagcgcaacc gcgagcgcct gctttctctg atcgcggacc agcttcatgc actgggcttc
      481 cggggcatgg gcgcgcagtc gctgaagccg aagcatgtgg aagcgctggt gaaccggtgg
      541 caggcagagt cgctctccgt cgggaccatc aagaaccgga tgacggtgct gcgctggtgg
      601 gcgcagaagg tgaaccgaca gaacgtcatc gcccggcaga acgaccacta tgggatcccc
      661 gagcgggtgt tcgtcagcac agactcgaag gcgcgccaga tcgacggcgc ggcgctcgag
      721 cgtatcaagg acgaccacgt ccggatgagc ctcgagctgc agcaggcctt cgggctccgg
      781 cgtgaggagg ccatcaagtt ccagccgagc tacgccgacc agggcgatta cctggtattg
      841 aaggacagct ggacgaaagg cggtaaggcc cgcgagatcc ccgtgcgtac cgtggcccag
      901 cgaacggttc tggatcgggc ccatcggctc gcaggccgcg gttcgctgat cccggccgag
      961 cgcaactacc gacagcagct gcgtgtgtac gagcgacaca cggcgaacgc cgggctctcg
     1021 aaactgcacg gcctccgcca tgcctatgcc cagggacgct atgaggagct gaccggctgg
     1081 aaagcccccg ctgccggcgg cccgacctcg aggtccctca cgcgagagca gcgcgtcgtc
     1141 gaccgcgagg cgcggctcgt catcagccgc gagctcggcc acgagcgcga gcagatcacg
     1201 accgtgtacc tggggcgctg aaaggaacgc gtcacctgcg agagtttccc gtttcgctgg
     1261 caacctgtgc gcaccagact cgggcgcagg ctctgaatcc gtccgggcaa tcatgaagcg
     1321 aacgcaagcg caccaacgcc atcttcccct cgtcgccgcc tgcattctgg gcgcactgct
     1381 tgcgcctctc gaggccaatg cggccgaggt cactgttgcg cgctactcga cggtgcagcc
     1441 cgcgccgagc atggcgcaac gggatcccct gtcagcaccg gtggtctcgg tactgccggc
     1501 ttccgtgact cgaattggag aagcggtcga gaccctgctc ggaccatccg gctatcgtct
     1561 ggcgtcaccc tttgccgccg aagcagagcg ggccgagctt ctgaatctgc cgctgccgga
     1621 gtcccaccgc acgctcgggc cactgccgct gcgtacggcg ttggcgatcc ttgccggacc
     1681 ggcg
//
LOCUS       DBNHOX010000702         4421 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1599069, whole genome shotgun sequence.
ACCESSION   DBNHOX010000702 DBNHOX010000000
VERSION     DBNHOX010000702.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1599069"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="AC16UZ_15260"
     CDS             complement(<1..602)
                     /locus_tag="AC16UZ_15260"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6248094.1"
                     /translation="MEADVIVLGTGAAGLTAALAAHGHGADVLVLEKSDKVGGTSAWS
                     GGMIWIPCNHHMRAAGIEDSREEALTYLSALSHDLIDPDLAEAFVDTGPEMVAWMESN
                     TPVQFQLVPDFPDYHPEHPGGKPGGGRSLECPLFAYETLEAHRDLVTVGYNYGTAPIT
                     MEESNLGRAVPIEVPEEERARRAERDERGCGQALMGRLFKG"
     gene            complement(619..1338)
                     /locus_tag="AC16UZ_15265"
     CDS             complement(619..1338)
                     /locus_tag="AC16UZ_15265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6248095.1"
                     /translation="MSADATPAAIGLLEGIATTRAIRRYLDEPIPDEALRDILFAATR
                     GPSGSNRQPFRFVVLTDSPKAKEAKRLIGSAARSMWSDKRRNDGYDQGSGADESSPKA
                     RMARTMQSYVDEFDRVPVLVLPCLVRYRAPTPMEGASVYPAVQNLLLAARALGYGGVI
                     TGFHGAVDAQLKALLGIPEEVFIAATVTLGRPRGAHGPVRRRPMRELVYGDEWGTPPA
                     WAEDPPGTRYTSAGPPKRKPT"
     gene            complement(1422..3542)
                     /locus_tag="AC16UZ_15270"
     CDS             complement(1422..3542)
                     /locus_tag="AC16UZ_15270"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR6248096.1"
                     /translation="MTLPPSLARTPRLDRWLRLDPDGSVAICTGKVEIGQGIKTALAM
                     IAADELDVALYRVRVRTADTALTPNELITAGSLSVEDSGGAIRVACATARAKLLDAAA
                     AVLGVPRDSLDVEDGTVTSSLTNQRTDYWSLPYDDLFSVEIQEPLPLKNPDSYRVVGR
                     PAQRLDLPAKVLGHPAFVHDMSLPDMLHGRLVKPPLPGARLERFPDDFQAPGLVAVVR
                     DGSFVGVVAEREEQAVRAAERLSAQCTWHRAPLSPLPSQMAAHLRENVAASFPVIHGG
                     PVAEPVPPAPEIRGTAKRLSATYTRPFQMHGSIGPSAALAQLESGRLTVYSHSQGVEL
                     LKIALADVLGMAEDSVRVVHAEGAGCYGHNGADDVALDAALLARAVTPRPVLTKWTRA
                     DEHGFEPYAPATVIEMTASLDDRGQILHWDHQAFGLTHMGRPRPSPGHSNLQSAWWLS
                     SPIPPVPREPLMFPEAGIHRNLQPIYDFPDQRLVKHFVADGPLRTSSTRSLGAFANVF
                     AIESFMDECAGAAGRDPFDFRLAHLRDPRAREVLETLRERAPDPAGGGRGIAMARYKS
                     RQAYVGLLVDVDVLDDARVSLKRALIVADAGLVIDPDGLRNQLEGGFIQAASWALKEQ
                     VDWDQNGVASRDWESYPIITFSEIPEIETHLIDRRHEPALGAGEASTGPTPAAIANAI
                     GNAAGVRVRDIPFTAEALRRAAAE"
     gene            complement(3539..4009)
                     /locus_tag="AC16UZ_15275"
     CDS             complement(3539..4009)
                     /locus_tag="AC16UZ_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019096755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6248097.1"
                     /translation="MATTLTVNGKAFTSDADPGTPLIFVLRNDLGLMGAKLGCGAEQC
                     GSCTVLADGEPIHSCTASLESISGRCIETLESLSRDGQPDAIQQAFIDENAAQCGYCT
                     AGIILAAKALLSKRPDPTRAEIVEALNGHLCRCGAHPRIIRAIERAAAARGQTS"
     gene            4141..>4421
                     /locus_tag="AC16UZ_15280"
     CDS             4141..>4421
                     /locus_tag="AC16UZ_15280"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6248098.1"
                     /translation="MPEEFPLPNFNTDLSGEVALVTGTTSGLGRRFALVLAKCGAKVA
                     LTGRRVERFADLEAEIKAFGGECFSTRLDMTDAQDAIDVVGRVEEALGTV"
BASE COUNT          678 a         1419 c         1541 g          783 t
ORIGIN      
        1 cccttgaaca gtcgtcccat cagcgcctgg ccgcaacccc gctcgtcgcg ctcggcccgg
       61 cgggcccgct cttcctctgg gacctcgatg ggcaccgcgc gacccaggtt ggattcttcc
      121 atcgtgatgg gcgcggtgcc gtagttgtag cccacggtga ccagatcgcg gtgcgcttcg
      181 agcgtttcgt aggcaaacag cgggcactcc agggaacgtc cgccgcccgg ctttccgccg
      241 gggtgctccg ggtggtagtc gggaaagtcc ggtaccagct gaaactgaac gggagtgttg
      301 gactccatcc aggcgaccat ttccgggcct gtgtccacga aggcctcggc gaggtcgggg
      361 tcgatgagat cgtgagacag ggccgataga tacgtgagcg cttcttctct ggagtcttcg
      421 atgccggcag cgcgcatgtg atggttgcag gggatccaga tcatgccgcc ggaccacgcg
      481 ctggtcccgc cgaccttgtc cgatttttcg agcaccagaa cgtctgcccc gtggccgtgt
      541 gccgctagag cggcggtcag gccggcggct ccggtgccca gcacgatgac gtctgcttcc
      601 atcgatacct ccactcggtc aggtgggttt tcgctttggg gggcccgcgc tggtgtaacg
      661 ggttcccgga gggtcctcgg cccaggcggg cggtgtgccc cactcatccc cgtagacgag
      721 ctcgcgcatg ggtcttcgcc ggacgggacc gtgggcaccc cgtggtctac ccagggtcac
      781 ggtggcggcg atgaacacct cttcaggaat accaagcagg gccttcagct gcgcgtccac
      841 ggcgccatgg aagcccgtga tgacgccacc gtagcccagt gccctggctg caagcagcag
      901 gttctgcaca gcggggtaca ccgaagcgcc ttccatgggc gtgggcgccc ggtagcgtac
      961 caggcagggt agcaccagca cgggaacccg gtcgaactcg tccacgtagg actgcatggt
     1021 gcgggccatg cgcgctttcg gggagctctc gtcggcgccc gagccctggt cgtaaccgtc
     1081 gttgcggcgc ttgtccgacc acatgcttcg cgccgcagag ccgatcaggc gtttggcttc
     1141 tttcgccttg gggctgtcgg tcagaaccac gaagcggaac ggctgccggt tggagccgct
     1201 cggcccgcga gtggcggcaa acaggatgtc gcgcagcgct tcgtcgggaa tgggctcgtc
     1261 taagtaccgg cggatggccc tcgtggtggc gataccttcc agcaggccga tggcggcggg
     1321 cgtggcgtcg gcggacattg tgagttcctg atggacgtgc ggcggcactc gattatggtg
     1381 tagaagagat ggggaacata gtagctcacg gcgccccagc gctactcggc ggccgcgcgt
     1441 cgcaaggcct cggcggtgaa cggtatgtcc cggaccctta cgccggcggc gttgcctatc
     1501 gcgttggcga tcgccgccgg cgtcgggccg gtgctggctt ctccggctcc cagcgcgggt
     1561 tcgtggcgcc ggtcgatgag atgggtctcg atttccggaa tctcgctgaa agtgatgatg
     1621 gggtagctct cccagtcccg gctggcgacg ccgttctggt cccagtcgac ctgctctttc
     1681 agcgcccagc tggcggcctg gatgaacccg ccttccaact ggtttctcag cccgtccgga
     1741 tcgatgacga gccccgcgtc ggccacgatc agggcccgct tgagcgatac gcgggcgtcg
     1801 tcgagaacgt cgacgtcaac cagcaggccc acatacgcct gccggctctt gtatcgggcc
     1861 atagcgatcc cccgcccgcc gccggcgggg tcaggcgcac gttcgcgcaa tgtctcgagc
     1921 acctcgcggg cgcggggatc gcgcaggtga gcgaggcgga agtcgaacgg atcgcggccg
     1981 gcggcgcccg cgcactcgtc catgaaggac tcgatggcga agacgttggc aaaggcaccc
     2041 aggcttcggg tagaggacgt ccgcaggggc ccgtcggcca cgaagtgctt gacgaggcgc
     2101 tgatccggga agtcgtagat gggctgcaga ttccggtgaa tgcccgcctc gggaaacatg
     2161 agcggctccc tcggcaccgg cgggattggc gatgacagcc accacgccga ctgcagattg
     2221 ctgtgtcccg ggctcggccg cgggcggccc atgtgggtga ggccgaacgc ctggtggtcc
     2281 cagtgcagaa tctgcccgcg atcgtcgagg ctcgcggtca tctcgatgac ggtggcgggc
     2341 gcgtatggtt caaagccatg ctcgtctgct cgcgtccatt tggtgagcac cggccgtggc
     2401 gtcacggctc gcgccagcag ggcggcgtcc agagcgacgt cgtcggcacc gttgtgcccg
     2461 tagcagcccg ctccttcggc gtgcacgacc cgaacgctgt cctcggccat gccgagcacg
     2521 tccgccaggg cgatcttcag cagctcgacg ccttggctgt gggagtacac ggtgaggcgt
     2581 ccggactcga gctgggccag ggctgcggac ggaccgatgg atccgtgcat ctggaacggg
     2641 cgcgtgtacg ttgcgctcaa gcgcttggcg gtcccccgga tctcgggtgc cggcgggacc
     2701 ggctcggcta ccggcccgcc gtggatgacg gggaagctgg ccgcgacgtt ctccctgaga
     2761 tgggcggcca tctgggaggg cagcggcgaa aggggcgccc tgtgccaggt gcactgcgcc
     2821 gagagacgct cggcggcgcg cacggcctgc tcctcccgtt cggccaccac cccgacgaag
     2881 ctgccgtccc tgacaacggc gacgagcccg ggtgcctgga agtcatcggg aaaacgttcc
     2941 agcctggcgc ccggcaaggg cggcttcacc agccggccgt gcagcatgtc gggcaggctc
     3001 atgtcgtgga cgaaggcggg atggccgagc acctttgccg gcagatccag ccgctgtgcc
     3061 gggcggccga ctacccggta actgtcgggg ttcttgagcg gcaggggttc ctgaatctcc
     3121 acggagaaca gatcgtcgta gggcagggac cagtagtccg ttcgctggtt tgtcaaagag
     3181 gacgttacgg ttccgtcctc cacgtccaga gagtcccggg gcacgccgag aactgcggcc
     3241 gcggcgtcga gaagtttcgc ccgagccgtt gcgcacgcga cacggatggc gccgccgctg
     3301 tcctcgacgg agaggcttcc cgctgtgatg agctcgttcg gcgtgagagc ggtgtctgcc
     3361 gtccgaacgc gaacgcggta cagcgcgacg tccagttcat ccgccgcaat catggccagc
     3421 gccgttttta ttccctgtcc tatctccacc ttccctgtgc agatcgccac gcttccgtcc
     3481 gggtcgagac gcagccatcg gtccagccgt ggcgttcgcg ccagggatgg gggcagtgtc
     3541 atgacgtctg tccacgtgcg gcagcggcgc gctcgatggc gcggatgatt cgcgggtgcg
     3601 cgccgcagcg gcacaggtgg ccgttcagcg cctcgactat ctcggcccgg gtcgggtccg
     3661 gccgtttgct cagcagcgcc ttggcagcca ggatgatgcc ggcggtgcag tagccgcact
     3721 gggcagcgtt ctcgtcgatg aatgcctgct ggattgcgtc cggctggccg tcccgggaca
     3781 gggactcgag cgtctcgata caacgcccgc tgatactttc cagcgacgcc gtgcagctat
     3841 ggatcggctc cccgtcggcc agcaccgtgc agctgccgca ctgctccgcg ccgcagccga
     3901 gcttcgcgcc catcaggccg agatcgttgc gcagcacgaa gatgagaggc gtgcccggat
     3961 ccgcgtcgct ggtgaaggcc ttgccgttta cggtaagagt cgtggccacg ttcggcaacc
     4021 tgcaggggtt ttcagaatta tgccagctgt cgccatcgtt gctcgcgagg atcgccggta
     4081 gtgaacggtg agcacaagtg ctaacttgcc tcgtttaatc aacgctgggg atagaccaac
     4141 atgccagaag agtttccttt gccgaacttc aacacggatc tcagcgggga agtggctctg
     4201 gtgacgggca cgacgtccgg gctcggccgt cgcttcgccc tggtgctcgc caaatgcggc
     4261 gccaaagtgg cgctcaccgg caggcgcgta gagcgctttg ccgacctgga agcagaaata
     4321 aaggccttcg gcggggagtg tttttccacg cggttggaca tgaccgatgc gcaggacgcc
     4381 atcgacgtcg tcggacgggt ggaggaagcg ctcggcaccg t
//
LOCUS       DBNHOX010000703         3069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_828725, whole genome shotgun sequence.
ACCESSION   DBNHOX010000703 DBNHOX010000000
VERSION     DBNHOX010000703.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_828725"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="AC16UZ_15285"
     CDS             complement(<1..400)
                     /locus_tag="AC16UZ_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248099.1"
                     /translation="MTAIDRTVFECLRFTTAVADRLVTGSRMSGPAFLENPYGEYARL
                     RARGEVIRNFSIGGWNVLGFEAVRDAMRDARFGVDVRKSPFVDRIVRLVSRGRPFRML
                     DEPTMLNIDPPDHTRLRRLARQGFLHRYVCA"
     gene            complement(534..2042)
                     /locus_tag="AC16UZ_15290"
     CDS             complement(534..2042)
                     /locus_tag="AC16UZ_15290"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HIR6248100.1"
                     /translation="METPLFLELLNLLASIFVFLLGLGLIAVIVIYIVDRTQTKHAVR
                     RNFPVIGRFRYVFEHLGEFFRQYFFALDREEMPFNRAERTWVYRAAKGEDTTVAFGST
                     RDLRPPGTVLFVNCAYPTLDEDAAPLGRVTIGPDCEHPYDCGSLFNISGMSFGAVSRP
                     AVTALSNGARMAGVWMNTGEGGLSSYHLDGGADIVFQIGTAKYGVRNERGELCDEKLA
                     EMAAHRQVKMFEIKLSQGAKPGEGGILPGNKVTDEIARIRLIPVGSDSISPNRHPEIE
                     NEGDLLDMIDRVRRATGKPVGFKTVIGAYGWLEDLFAEINARGPASAPDFITIDSADG
                     GTGAAPMSLIDYMGLPIRESLPLVVDLIEAYGLRERIRVVASGKMVTPGEAAWAVCAG
                     ADFVVSARGFMFALGCIQALQCNKNTCPTGVTTHDPDLQRGLDPVDKAERVRRYAQHM
                     HRQLGMIAHSCGVAEPRQLKRFHCRIVTQTGISVPLDELYPPLVRDPARLSA"
     gene            complement(2133..>3069)
                     /locus_tag="AC16UZ_15295"
     CDS             complement(2133..>3069)
                     /locus_tag="AC16UZ_15295"
                     /inference="COORDINATES: protein motif:HMM:NF014722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HIR6248101.1"
                     /translation="LRQLADFTIRTHFPHLAPGSAPLARDDYLAWFAEVRDATVEMVV
                     EWMRVGFVHGVMNTDNMSILGQTIDYGPYGWLEDYDPNWTPNTTDAAGRRYRFSYQPG
                     IAQWNLTRLAEAIYPLVEAAEPLEELLAGFEKRYVESWQSMMARKLGLRSPQPADVVL
                     LNELDEVLQLAETDMTIFFRRLADLSPADPASDPVTVVREAYYLPDQLAGETLERIGN
                     WLEDYVARLRQEGVGNEERRQAMNAVNPKYVLRNYLAQLAIDDAESGDFALVGELLDL
                     FRRPYDEQPARERFAARRPDWARTRVGCSMLSCSS"
BASE COUNT          568 a          986 c          979 g          536 t
ORIGIN      
        1 gcgcgcagac gtaccggtgg agaaagccct gacgggccag ccgacgcagc cgggtgtggt
       61 ccggcgggtc tatgttgagc atcgtcggtt catcgagcat tcgaaacggc ctgcctcggc
      121 tcacgagccg cacgatcctg tccacgaaag ggctcttgcg aacatcgacg ccgaaacgcg
      181 catcccgcat ggcgtctcgg accgcttcga agcccaggac gttccatccg ccgatgctga
      241 agtttcgtat gacctcaccc cgggcgcgga gccttgcata ttcgccatag gggttctcca
      301 ggaaagcggg gccgctcatc cggctgccgg tcaccaggcg atcggccacg gccgtggtga
      361 agcgtaggca ctcgaacacc gtcctgtcga tggccgtcat ggcgggactc ccgttagaag
      421 cgctggcctg cgccgacacc tcattgttgc acccaccgat gccccgacaa ggcttcgaac
      481 gcgaacctgt gcgccgcctc acagattgga ggcccagcgc ggaccagctc cgactatgcg
      541 gacagccggg cgggatccct gacgagcggc ggatacagct cgtctaaagg cacggaaatt
      601 cccgtctgcg tgacgatgcg gcaatgaaag cgtttgagct ggcggggctc ggccacaccg
      661 caggagtggg cgatcatgcc gagctggcgg tgcatgtgct gcgcgtaacg acgaacgcgt
      721 tccgccttgt ctacagggtc cagaccccgc tgcaggtcgg ggtcgtgcgt ggtcacgccg
      781 gtggggcagg tattcttgtt gcactgcagc gcctgtatgc agcccagggc aaacatgaag
      841 ccccgggcgg agactacgaa gtcggcaccc gcgcacacgg cccaagccgc ctctcccggt
      901 gtgaccatct ttccgctggc gacgaccctg atgcgctcgc gcagaccgta cgcctcgatg
      961 aggtcgacca cgagtggaag actttctctt atgggaagac ccatgtagtc gatcaggctc
     1021 atgggagcag ccccggtccc gccgtcggcg ctgtcgatgg tgatgaagtc cggtgccgag
     1081 gccggccccc gagcattgat ttctgcaaag aggtcctcga gccagccgta cgccccgatc
     1141 acggtcttga agcccaccgg cttccccgtc gcccgcctga cgcgatcgat catgtccagc
     1201 aggtctccct cgttctcgat ctcagggtga cgattggggc tgatggagtc cgaaccgacc
     1261 gggatcagac gaatccgcgc gatctcgtcg gtaactttgt tacccggcag gatgccgcct
     1321 tcccccggtt tggcgccctg ggagagcttg atctcgaaca tcttcacctg ccggtgcgcg
     1381 gccatctcgg cgagcttttc gtcgcaaagc tcgccgcgct cgttgcgcac gccgtacttg
     1441 gcggtcccga tctgaaagac gatgtccgcc cctccgtcga ggtggtatga ggacagcccg
     1501 ccttcaccgg tgttcatcca cacgcccgcc atgcgcgcgc cgttggaaag ggcggtgacg
     1561 gccggtcgcg agacggcgcc gaagctcatg ccggagatgt tgaaaagaga accgcagtcg
     1621 taggggtgct cgcaatcagg cccgatggtg acgcggccta agggagccgc gtcctcgtcg
     1681 agggtggggt aggcgcagtt gacgaacagc accgtgcccg gcgggcgcag atcgcgcgtg
     1741 gagccgaagg ccaccgtggt gtcctcgccc tttgcggcgc gatacaccca cgttctctcc
     1801 gctcggttga acggcatctc ctcgcgatcc agcgcaaaaa agtactgtcg gaagaactct
     1861 ccgaggtgct cgaacacgta acgaaagcgc ccgatgacgg gaaaattgcg gcgaaccgca
     1921 tgtttcgtct gggtccgatc gacgatgtag atcacgatca ccgcgatcag cccgaggcca
     1981 agcagaaaga cgaagatgct ggcgagcagg ttcaaaagct cgaggaataa gggcgtttcc
     2041 atgggcagcc tcccgatagc cacggaccga gaatagagca ccgaaagcgg acaatcgacc
     2101 cgtcgggcgc cgcccggact gggggcggga tatcaggagc tgcaggacaa catggagcag
     2161 cccacacgcg ttcttgccca gtccggccgc cgggccgcaa accgctcacg tgccggctgc
     2221 tcgtcgtagg ggcgcctgaa gagatcgagc agctcgccaa ccagagcgaa gtcgccggac
     2281 tcggcatcgt cgatggcgag ctgggccagg tagttacgca ggacgtactt cggattcacg
     2341 gcgttcattg cctggcgccg ctcctcgttc cccacacctt cctgccgcag ccgggctacg
     2401 tagtcctcga gccagttgcc gatgcgctcg agggtctcgc cggcgagctg atcgggcaga
     2461 tagtaagcct cgcggaccac cgtcaccgga tcgcttgccg gatcggccgg actcagatcg
     2521 gccaggcggc ggaaaaagat ggtcatatcg gtctcggcca gctgcagcac ctcgtcgagc
     2581 tcgttcaaaa gcacgacgtc ggccggctgg ggcgatctca gaccgagctt ccgagccatc
     2641 atgctctgcc atgactccac gtagcgcttc tcgaatccgg ccagcagttc ctccagcggc
     2701 tctgcggctt caacgagagg atagatggcc tccgccagtc gggtgaggtt ccattgagct
     2761 attcccggct ggtacgaaaa ccggtaccgc cggcctgcag catcggtcgt gttgggcgtc
     2821 cagttcggat cgtagtcctc cagccagccg tagggcccgt agtcgatggt ctggcccaga
     2881 atggacatgt tgtccgtgtt catcacgccg tgcacgaacc cgacacgcat ccattccacg
     2941 accatctcga cggtggcgtc acgcacttcc gcgaaccagg ccagatagtc gtcccttgca
     3001 aggggcgcag agcctggtgc cagatggggg aagtgtgttc gtatcgtgaa gtcggcgagc
     3061 tgcctgagc
//
LOCUS       DBNHOX010000704         2783 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_571772, whole genome shotgun sequence.
ACCESSION   DBNHOX010000704 DBNHOX010000000
VERSION     DBNHOX010000704.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_571772"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="AC16UZ_15300"
     CDS             complement(<1..187)
                     /locus_tag="AC16UZ_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248102.1"
                     /translation="MLVGVQCSGPPDNAFADYLSRVQRLLETDVSVDAEGSLPRYPNR
                     GLLRRDVASLSIDVLDFI"
     gene            complement(210..482)
                     /locus_tag="AC16UZ_15305"
     CDS             complement(210..482)
                     /locus_tag="AC16UZ_15305"
                     /inference="COORDINATES: protein motif:HMM:NF016351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HIR6248103.1"
                     /translation="MLQVVFHLSGCRSLKEKRQRLKGIRDRFGRSSNVAVCESAYQDS
                     LQRAEWSFVASASSRAVVEKSLAEIERSLSLSVDAELVDVRRRWLV"
     gene            complement(491..1015)
                     /locus_tag="AC16UZ_15310"
     CDS             complement(491..1015)
                     /locus_tag="AC16UZ_15310"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6248104.1"
                     /translation="MPTLTTERLLLRPLDDSDISDLYEFWNDPEVRRYLWEDQIMSRD
                     AVAQIVADSVRRFQALGSGFFAIEMAANPGSLVGFCGHREFEDGEQVELLFGIVPEFW
                     GEGFVTEAAMEVLRHGFETCGIVRVIAATETPNQRSVRVLQRLGMVFEERRKHHGLDT
                     VFYSLTSEGFASSG"
     gene            1094..1885
                     /locus_tag="AC16UZ_15315"
     CDS             1094..1885
                     /locus_tag="AC16UZ_15315"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6248105.1"
                     /translation="MSEIAPAVPSATILMLRDGPRGLEVFMVVRHHEIDFASGALVFP
                     GGKADPGDFDEGLLPRLDGADADPTLRGIQVAAIREAFEECGILLARAAGEEGLIGGE
                     RLAELEPYRNALHAGELSMLEFAEREDIRLACNELTHFAHWITPENLPKRFDTHFYLA
                     MAPADHLALHDGHESVDSVWITPAQAIADAESGTRTVIFPTLRNLAKLGFSDTAEVAV
                     AAAATDTVVPVLPSIEQRDDGTYICIPPEAGYDISEERVPQPRSG"
     gene            complement(1941..2714)
                     /locus_tag="AC16UZ_15320"
     CDS             complement(1941..2714)
                     /locus_tag="AC16UZ_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248106.1"
                     /translation="MKRIILALSLMTAPLLAGAEEAAPTPMYVAATNLTVSGHPGAQN
                     RFETFIKRAAEAHAKLDTKRHWFAWQQEMGNPRSYTLARTFGSMTAMDETPMNPIAEA
                     FGPEEIAKLTPDHEVVASVERGVWVQRPDLAVMPDSPVGQRVIWLQATVRAGENERFE
                     AYVAKVAEATREVAPEVRYATFAPVFGSQNTYVFSVPTSYAQLDEAPVMSVSERLREA
                     FGEREARRLEAERAATVESLVQLLVRPRPDLAYQPPAEE"
BASE COUNT          504 a          958 c          850 g          471 t
ORIGIN      
        1 cgataaagtc cagaacgtcg atgctcagtg acgccacgtc gcggcgcagg aggccccgat
       61 tcggataacg aggcaaggag ccttcagcgt ccacggaaac gtcggtttcg agcaggcgct
      121 gaacgcgaga caggtagtcg gcaaaggcgt tatccggcgg gccgctgcac tgaacgccga
      181 ccagcacacc cccggccagc gcgaagcgcc tacaccagcc agcgacggcg gacgtccaca
      241 agctctgcat cgacgctgag actcaggctg cgttcgatct cggcaaggct cttctcgacc
      301 acggctcggc tgctcgccga cgccacgaac gaccactctg ctcgctgcag ggagtcctgg
      361 taagcgcttt cgcatacggc cacgttcgag cttcgcccga accggtcccg gatccccttg
      421 agacgctgac gcttttcctt gagggagcga cacccgctga gatggaagac cacctgaagc
      481 atcgcgacgt tcacccggaa gacgcgaacc cttcgctggt cagtgagtag aagaccgtgt
      541 ccagaccatg gtgcttacgt cgttcctcga aaaccatgcc gagcctttga agaacccgga
      601 cggatcgctg attcggcgtc tccgtggcgg cgatcaccct aacgatgccg caggtttcga
      661 agccgtgccg cagcacctcc atggccgcct cggtcacgaa tccctctccc cagaactccg
      721 gcacgatgcc gaacagcagc tccacctgtt cgccgtcttc gaactcccgg tgaccgcaga
      781 atccaaccag cgagcccgga ttggcggcca tttcgatggc gaaaaagccg gagccgagcg
      841 cctggaaccg gcgcacgctg tccgccacga tctgagcgac cgcgtcgcgc gacatgatct
      901 ggtcctccca gaggtagcgg cgcacttccg ggtcgttcca gaactcgtac agatcggaga
      961 tatccgagtc gtccagcggc ctgagcaaca gccgctcggt cgtcagcgtc ggcatgtgaa
     1021 acctccctcc cgcgcccgag tttgccatag cccgggacat aagcgaacat cccggccctt
     1081 cgagggagac acgatgagcg agatcgcccc ggccgtgccg tcggccacca tcctcatgct
     1141 gcgcgacgga ccgcgggggc tggaggtctt catggtggtt cgccaccacg agatcgactt
     1201 cgcctcgggc gcgctcgttt tccctggcgg gaaggcggat cccggcgatt tcgacgaggg
     1261 cctgctgccc cggctggacg gcgcggatgc ggaccctacc ctgcgcggca ttcaggtcgc
     1321 ggccatccga gaggccttcg aggagtgcgg catcctgctt gccagagccg caggcgaaga
     1381 aggcctcatc ggcggcgagc ggctcgccga gctggaaccg taccgaaacg ccctgcacgc
     1441 cggagagctg tcgatgctgg agtttgcaga gcgggaagac atccgtctcg cctgcaacga
     1501 gctgacccac tttgctcact ggatcacccc ggagaacctg ccaaagcgat tcgacaccca
     1561 cttttatctg gccatggctc ccgccgatca tctggccctg cacgacggtc acgagtccgt
     1621 ggattcggtc tggatcacgc ccgcccaggc gatcgccgat gcggaaagcg gcacccggac
     1681 cgtcatcttc ccgacgcttc gcaacctcgc caagctggga ttcagcgaca ccgccgaggt
     1741 tgcggttgct gcggccgcaa cggacaccgt agtgccggtt ctcccgagca tcgagcagcg
     1801 cgacgacggg acctacatct gcatcccgcc cgaggcaggc tacgacatct ccgaagagcg
     1861 agttccgcaa ccccggtcag gctgagcaac agcgatgcac gggcacccgc caacgaccgg
     1921 gcgcccatgc cggctgggtg ctactcctcc gccggtggct gataggccag gtcgggtctg
     1981 ggtcggacca gcagctggac cagagattcc acggttgccg ctcgctccgc ctcgagacgt
     2041 cgcgcctcgc gctcgccgaa cgcctcgcga agccgctccg atacggacat gacaggggct
     2101 tcgtccagct gggcatagct ggtgggcaca gagaagacgt aggtgttctg ggacccgaag
     2161 acgggcgcaa aggtcgcgta gcgaacctca ggcgccactt cccgggtggc ttccgccacc
     2221 ttggccacat aggcttcaaa gcgctcgttc tcgcccgctc tcaccgttgc ctgcagccag
     2281 atcacccgct gccccactgg cgaatccggc atgacggcca gatcgggccg ttgcacccaa
     2341 acacccctct cgacgctggc cactacctca tggtcgggcg tgagcttggc aatttcttcc
     2401 gggccgaaag cctcggcgat cgggttcatc ggcgtctcgt ccatggcggt catgctgccg
     2461 aacgtgcgcg ccagcgtgta ggagcgcggg ttgcccatct cctgctgcca ggcgaaccaa
     2521 tgccgtttcg tgtccagctt ggcgtgggct tcggccgccc gcttgatgaa ggtctcgaaa
     2581 cggttctgcg cgccgggatg accgctcacc gtcaggttgg tagcggcaac gtacatgggc
     2641 gtcggcgcgg cctcttccgc acccgccagc agcggtgccg tcatcaggct cagcgccaga
     2701 attattcttt tcatgatcgc tcgtcctcgt atgctttggt tgtgtcagtc cgggtgcaaa
     2761 cacgaccacc acctgcaaac ggc
//
LOCUS       DBNHOX010000705         6461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1022343, whole genome shotgun sequence.
ACCESSION   DBNHOX010000705 DBNHOX010000000
VERSION     DBNHOX010000705.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1022343"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..595
                     /locus_tag="AC16UZ_15325"
     CDS             101..595
                     /locus_tag="AC16UZ_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248107.1"
                     /translation="MSMGIARLAAAGCLAFLIPVASPDAIAAAYDFDDAAYLAPASSL
                     PDRADTVERNDLEQRTIAECLADESVCQSRLIGIRHLLTKAQSLPIEKQIRLVNRYVN
                     KRRYRDDRSVGDYHAVLAVRSAPGSTWLLESDNTIQRGGAWGYRYVFAINEDSIWDHE
                     GADG"
     gene            627..863
                     /locus_tag="AC16UZ_15330"
     CDS             627..863
                     /locus_tag="AC16UZ_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248108.1"
                     /translation="MRTLILAACCLIAGIATADEAVVKVYSLSVQERLESLELIDVAA
                     EKAPAGSDAPMDAELAEILREVEALEQASALVRD"
     gene            complement(853..1410)
                     /locus_tag="AC16UZ_15335"
     CDS             complement(853..1410)
                     /locus_tag="AC16UZ_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248109.1"
                     /translation="MAYGWIVLAGISLAVVDGGLARAEAPPEPDHLPAEAASGLPPLR
                     CEAEQTGGFRDYPGEDEGYEPALFNPSRIFLEENVVFMLNLPVGNGAPDLYLVMRVGE
                     ELETEFACRQVRGADGAMGYSCVNTPPSEMLLINRETLRFTRTAVGGWTFAGASESLS
                     GDSIFIEYGACVPDDDQVRRPKFSP"
     gene            1467..2930
                     /locus_tag="AC16UZ_15340"
     CDS             1467..2930
                     /locus_tag="AC16UZ_15340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6248110.1"
                     /translation="MRSIRPVIIALAAALPLAAHGDPFEHEWCVYPTDHFEILSDLRP
                     RQIKRLAAELEVFRQTAAQFIPGTGAANLPIRIVVFRDRDDFRETMNSPKFGGFMQPS
                     LRGATLVLGPQDANRLLHTTARHEYSHYLLRNRIEVSFPVWYDEGLASFLSTMSTRRR
                     SVVVGEAPARHLDSRLRRSAVGLAELIEERDVYTWEPNKLNDFYTGAWTLVHYLTLGH
                     LAGFEDRRERLSRYLNEVVTPFPAAMDLSYDQLEKELLTYTNRRKLPTIELKRPESSA
                     ARADVGCLRDDERDYELAVAMIKKRPETALDIFAGLQRKHPADVRHLVGLATASAELN
                     RPEDAKGYAAAALKLAPDDPAAQIEYGTRLIRNCIMMRALGCDERWQQAVKLFRSGVR
                     ADPERFDAVMGLGLSYLHSGRPGDALNYLKIAYQKAPWAPHLNFYLGETYRLVGDPRA
                     RQHLLNARNWSDQDVWRRLATAALEQMELSSGERIAW"
     gene            complement(2905..3456)
                     /locus_tag="AC16UZ_15345"
     CDS             complement(2905..3456)
                     /locus_tag="AC16UZ_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248111.1"
                     /translation="MLLTWRTAVELFAMIERGPGPMMIRHGWLLLLLLMGCSSTVVLD
                     RDVPSGFDLDGEWVVDAAASEVAPGTRRLRARGIGISLVAHDFPVLRCERMTIEQSAD
                     SMGIEYDGASYRDVSWGVRQRGLWEVNAGWEEGVFRILSKARDAKAEEMMTLADSGRR
                     LIVQVVVESEGDTIDITRQFVRR"
     gene            complement(3475..3915)
                     /locus_tag="AC16UZ_15350"
     CDS             complement(3475..3915)
                     /locus_tag="AC16UZ_15350"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6248112.1"
                     /translation="MAVASNISFSEKVAQGFMRAPSGAGGGLPEFLGVKITDAGPGTM
                     TGQFEVRPELLTPIGNMHGGVLSAFCDHMLGCVCYPAMKPGQWAATTEFKINLTAPVT
                     QGVVEARAEIVNMSRTQAVVRLDLTNEGRVVAMAQGTVTVRDPR"
     gene            complement(3924..4907)
                     /locus_tag="AC16UZ_15355"
     CDS             complement(3924..4907)
                     /locus_tag="AC16UZ_15355"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6248113.1"
                     /translation="MKYGLILGSPSIQGQIDTARRAEAAGFESVWTIEFFNMHGLVRL
                     AAVATATERVKLGTAIAYAFMRTPMLAASAAMDIDEISGGRMILGLGSGTERMNRDWY
                     SMPFDDPPAPRIKDAIGLVRAAFAAQKGGGLVYEGPYYQVRIPAYARPGAVRESIPVC
                     LAAVNRGMIRAAAAVADGLIGHPVYTRRYITDVVLPELENGSCELIPYIITAVADTTE
                     QARNEARGQIAFYYTTRLYHSILEPHGWRDTGEAIAAAFRKGDFSAMADAVSDEMVDA
                     IAIAGTPDEVRDQITQWEGLADHVILYSPSVGMRGGRVQENLDAIVDTFAA"
     gene            complement(4937..6007)
                     /locus_tag="AC16UZ_15360"
     CDS             complement(4937..6007)
                     /locus_tag="AC16UZ_15360"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR6248114.1"
                     /translation="MVQTDVRKLEPMELPLPEIDEDSALLKLEACGICGSDHEQYEGL
                     LRTPMPVVPGHEPLGTIAKIGDRAATRWGVDQGDRVAVETMISCRHCRPCLSGSYHLC
                     DHRLIYSYVPVSEAPGLWGGYSQYMYLHPNSIVHRVSPELPAEIGVMFNPLGAGFRWA
                     VELPATRPGDTVVILGPGQRGLASVIACREVGAGTIIVTGLSADEKKLQLAREFGADY
                     TIDVDSEDTVKRVKAITDGRGADVVVDVSAYATKPVSDALSLVRPGGTVVLAGVKGFK
                     AVEDFVSDLVVLKEINIKGAIGVTSSGYRSAIRLLESGRVPVERMHTHDFALADAELA
                     IRTLAREVPGDESVHSCLIPPH"
     gene            6124..>6461
                     /locus_tag="AC16UZ_15365"
     CDS             6124..>6461
                     /locus_tag="AC16UZ_15365"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6248115.1"
                     /translation="MARTPTVIVVGGGNAALCAAISAAEHGADVRLLERAPETLAGGN
                     SRFTAGAMRVTYDGVEDLKRLMPELTDAEIARTEFGSYPRDAFFDDMGRITHYRTDPE
                     LAETLIDSSFS"
BASE COUNT         1165 a         2259 c         1987 g         1050 t
ORIGIN      
        1 aatgaactct gcgcgaccgg catggaacgc acccccgcaa aaatagtagc gccgccgtgg
       61 gaccattgca caccgtgctt ccatgagaga ccatgaatcc atgagcatgg gcatcgcgcg
      121 gctggccgcg gccggctgcc tggcgtttct gatcccggtg gcctccccgg acgccatcgc
      181 agccgcttac gatttcgacg acgccgccta cctcgccccg gcaagctcgc tgcccgaccg
      241 ggccgacacc gtcgagcgca acgatctcga gcaacgcacg atcgccgagt gcctggctga
      301 cgagtctgta tgccaatccc gactcatagg gattcgccac ctgctgacga aagcccagtc
      361 cctgccgata gaaaaacaga tccgtctggt caaccggtac gtcaacaagc gccgctaccg
      421 ggatgaccgc agcgtcgggg actaccacgc ggtgctggca gtgcgcagcg cgcccggctc
      481 gacctggctg ctcgagtcgg acaacacgat ccagaggggc ggtgcctggg ggtaccgcta
      541 cgtcttcgcc atcaacgaag attccatctg ggaccacgag ggagcagacg gctgaccccg
      601 gagaggtcgg ggcatgggag cagagcatga gaacgttgat actggcagcg tgctgcctga
      661 tcgcaggcat cgcaacggcc gacgaggccg tggtcaaggt ctactcgctt tccgttcagg
      721 aacgccttga aagcctggag ctgatcgacg tggccgccga gaaagcgccg gcggggagcg
      781 acgcgccgat ggatgcggag ctggccgaaa ttctcagaga ggtggaagcc ctggaacagg
      841 cttccgcgct ggtcagggac tgaatttggg ccgccgcacc tgatcgtcgt caggcacgca
      901 ggcgccatac tcgatgaaga tcgagtctcc cgaaagggac tcgctggcgc cagcaaacgt
      961 ccagccgcct accgccgtgc gcgtgaagcg cagcgtctcg cggttgatga gcagcatctc
     1021 cgatggcggc gtgttcacgc agctgtagcc cattgccccg tccgcgccgc gaacctggcg
     1081 acaggcgaac tcggtttcca gctcctcacc aacccgcatg accagataca ggtcgggcgc
     1141 gccgttgccc accggcaggt tcagcatgaa caccacgttc tcctcgagaa agatgcggct
     1201 cgggttgaac agggccggtt cgtacccttc gtcttctccg ggatagtcgc gaaacccgcc
     1261 cgtctgctcg gcctcacaac gaaggggcgg cagtccggag gccgcctcgg cgggtaagtg
     1321 gtcgggctcc ggcggcgctt ccgcacgggc cagcccccca tcgacgacgg caagcgagat
     1381 acccgcaaga acgatccatc cgtatgccac ttcagaactc cgcgccactc gaatagtcta
     1441 aaacccatct ccggtgaccg tcgagcatgc gcagtatccg cccagtcatc atagccctgg
     1501 ccgccgccct gccgctggcc gcccacggcg atcccttcga gcacgagtgg tgcgtctacc
     1561 cgacggacca cttcgagatc ctcagcgacc tgcggccgcg ccagatcaaa cgccttgccg
     1621 ccgagctaga agtcttccgc cagacggcgg cccagttcat tcccggcacc ggagccgcaa
     1681 acctgcctat ccgcatcgtg gtgtttcgcg accgggacga tttccgcgag accatgaact
     1741 cgcccaaatt cggcggcttc atgcagccgt ctctccgcgg ggccaccctt gttctcggcc
     1801 cccaggacgc gaaccgcctg ctccacacca ccgcccggca cgaatactcc cactacctgc
     1861 tgcgcaaccg gatcgaggtc agctttcccg tctggtacga cgaggggctg gcgtcgtttc
     1921 tgtcgacgat gtccaccagg cgccggtcgg tcgtggtcgg cgaggcgccc gcgcggcatc
     1981 tcgattcccg gctgcgccgc agcgcggtcg ggcttgctga gctcatcgag gaacgcgacg
     2041 tctacacgtg ggagccgaac aagctcaatg atttctacac cggcgcgtgg acgctcgtgc
     2101 actatctcac gctgggtcac ctggccggct tcgaggatcg acgggaaagg ctgagccggt
     2161 atctcaacga ggtcgtcacc cccttcccgg cggcgatgga cctctcctac gaccagctcg
     2221 aaaaggagct gctgacctat accaaccgac gcaagctgcc gaccatcgag ctcaagcgcc
     2281 cggagagctc cgcagccagg gccgacgtcg gctgtctgag ggatgacgag cgggactacg
     2341 agctggccgt ggcgatgatc aagaagcgtc cggagaccgc cctcgacatc tttgccggcc
     2401 tgcagcgcaa gcacccggca gacgtccgcc acctggtcgg gctggccacc gcatcggccg
     2461 agctgaatcg gccggaggac gccaaaggat acgcggccgc cgcgttgaag ctcgcgcccg
     2521 acgaccccgc cgctcagatc gagtacggca cccgcctgat ccggaactgc atcatgatga
     2581 gggcgctggg ctgcgacgag aggtggcagc aagcggtgaa gctgttccga agcggcgtgc
     2641 gtgccgatcc cgagcgattc gatgcggtca tgggactcgg cctctcctac ctccactccg
     2701 ggcggccggg agacgcgctc aactacctga agatcgccta tcagaaggcg ccatgggcgc
     2761 cgcacctgaa cttctacctg ggcgagacct accggctggt cggcgatccc cgggcgcggc
     2821 agcacctgct caacgcccgc aactggtcag accaggacgt gtggcgccgg ctggccaccg
     2881 ccgcgctcga acagatggag ctttctagcg gcgaacgaat tgcctggtga tgtcgatggt
     2941 gtccccttcc gactccacca ccacttgcac gatgagccgg cggccggaat cggcgagggt
     3001 catcatctcc tccgctttgg cgtcccgcgc cttggaaaga atgcggaata ccccttcctc
     3061 ccagccggcg ttcacttccc acaacccgcg ctgacggaca ccccaggaga cgtcgcggta
     3121 ggacgcgccg tcgtactcga tgcccatgga atccgcgctc tgctcgatgg tcatgcgctc
     3181 acagcgcagc acgggaaagt cgtgggcgac cagggaaatg ccgatgcccc gcgcccgcag
     3241 ccgccgggtt ccgggcgcga cttcgctggc cgccgcatcc accacccact cgccgtcgag
     3301 gtcgaatccc gacggcacgt cccggtcgag aactaccgtg gacgagcaac ccatcagcag
     3361 caggagcaac agccaaccgt gtcgaatcat catgggcccg ggtcctcttt cgatcatcgc
     3421 gaacagctcg acggccgtcc gccatgttag cagcatggca gcgccaggcg ctggtcagcg
     3481 cggatcgcgt accgtcaccg tgccctgagc catcgccacg acccgccctt cgttggtcag
     3541 gtcgagccgc accacggcct gagtccgcga catgttgacg atctccgcac gagcctccac
     3601 gacgccctgg gtgaccggtg ccgtgaggtt gatcttgaac tcggtggtgg ccgcccactg
     3661 gcccggcttc atggccggat agcagacgca tcccagcatg tgatcgcaga aagcggatag
     3721 cacgccgccg tgcatgttgc cgatgggcgt gagcaattcc ggcctgacct cgaactgccc
     3781 ggtcatggtg cccggacccg cgtcggtgat cttcacgccg aggaactcgg gcaaaccgcc
     3841 cccggccccg gacggcgccc tcatgaaccc ctgagccacc ttctcactga aactgatgtt
     3901 cgacgcaacc gccatgtttt acctcaagct gcaaacgtgt ccacgatggc atccagattc
     3961 tcctgaaccc gaccgccgcg catccccacg ctgggcgagt agagaatgac gtggtcggca
     4021 agaccctccc actgcgtgat ctggtcgcgc acctcgtccg gcgtccccgc aatggcgatg
     4081 gcgtcgacca tctcgtcgct caccgcgtcc gccatggcgg aaaaatctcc tttgcggaag
     4141 gcggccgcaa tggcttctcc cgtgtcccgc cagccatggg gctctaaaat ggagtggtaa
     4201 agccgcgtgg tgtagtaaaa ggcgatctgc ccgcgcgctt cgttgcgcgc ctgctccgtc
     4261 gtgtccgcca ccgccgtgat gatgtaaggg atcagctcgc acgagccgtt ctccagttcc
     4321 ggtaagacca cgtcggtgat gtacctgcgg gtgtagaccg ggtggccgat cagcccgtcc
     4381 gccacggcgg ccgcagcgcg gatcatgccc cggttcacgg ccgcgaggca caccgggatc
     4441 gattcccgca cggcgccggg gcgcgcatac gcgggaatcc tgacctgata gtaagggcct
     4501 tcgtagacaa gccccccgcc cttctgagcg gcaaacgccg cccgcacgag gccgatggcg
     4561 tccttgatcc tgggcgccgg cgggtcgtcg aacggcatgg agtaccagtc gcggttcatc
     4621 ctctcggtgc cgctgcccaa tcccagaatc atgcgcccgc cggaaatctc gtcgatgtcc
     4681 atggcggcag atgcggcgag catcggtgtg cgcatgaagg catacgcgat ggcggtgccc
     4741 agcttgacgc gctccgtcgc cgtggccacg gcggcaaggc gcaccagccc gtgcatgttg
     4801 aagaactcga tggtccaaac ggattcgaag cccgcggctt ccgcccggcg agccgtgtcg
     4861 atctgtccct ggatggaggg agatcccaga atcaggccgt atttcatgac gtcttctctc
     4921 cgtggcaatc cgggtttcag tgcgggggga tcaggcagga gtgaacggac tcgtcgccag
     4981 gaacctctcg agccagggtt cgtatcgcaa gctccgcgtc cgccagcgcg aagtcatggg
     5041 tgtgcatgcg ctccaccggc acgcgcccgg actccagcag gcggatggcg ctgcgataac
     5101 cggacgaagt tacgccgatg gcccccttga tgttgatttc cttgagcacc acgagatccg
     5161 agacgaaatc ctccaccgcc ttgaagccct tcacgccggc cagcaccacc gtgccgcccg
     5221 gccgaaccag cgacagcgca tcggacacgg gtttcgtcgc atatgcgctc acgtcgacga
     5281 ccacatcggc gccgcgcccg tccgtgatcg ccttgacccg ctttaccgtg tcctcgctgt
     5341 ccacgtcgat ggtgtaatcc gccccgaact cccgggcgag ctgaagcttc ttctcgtctg
     5401 cggaaaggcc cgtcacgatg atggtcccgg cccctacctc ccggcaggca atcacgctgg
     5461 caagcccccg ctggccgggg ccgagaatga ccaccgtgtc tcccggccgg gtcgccggca
     5521 gctccaccgc ccagcggaag cccgcgccaa gcgggttgaa catcacgccg atctcggccg
     5581 gcagctccgg gctcacccga tgcacgatgc tgttcggatg cagatacatg tactgtgagt
     5641 agccgcccca cagccccggc gcttcgctca caggcacgta cgagtagatg agccggtggt
     5701 cgcagaggtg atacgagccg ctcaggcacg gccggcagtg acggcaggag atcatcgtct
     5761 ccaccgcgac ccgatcgccc tgatcgacgc cccagcgagt cgccgcccgg tcaccgatct
     5821 tggcgatggt gcccaggggt tcgtgaccgg gaaccacggg catgggcgtg cgcagaagac
     5881 cctcgtactg ctcgtgatcg ctgccgcaga taccgcaggc ttccagcttc agcagcgccg
     5941 aatcttcgtc gatttcaggc agcgggagct ccatgggctc gagcttgcgc acgtcggtct
     6001 gaaccatcgc gaacgacatt ctcggcaacg tcaacggtgc ggctccccct cgctcgaagc
     6061 gctcggtcgc gcctcctccc gtgatagtct ccacgagact acgggcaacg gcctgacaga
     6121 gcaatggcaa gaacccccac cgtcatcgtc gtcggcggcg gcaacgcggc cctttgcgcc
     6181 gcgatcagcg cggccgagca cggcgccgac gttcggcttc tcgaacgcgc cccggagact
     6241 ctcgccgggg gcaactcccg cttcacggcc ggcgccatgc gggtgaccta cgacggcgtc
     6301 gaggacctga aacggctgat gccggagctc accgacgcgg aaatcgcacg caccgagttc
     6361 ggcagctatc cgcgcgatgc gttcttcgac gacatgggcc gcatcaccca ttaccgcacc
     6421 gaccccgagc ttgccgagac gctcatcgac tcgagctttt c
//
LOCUS       DBNHOX010000706         1967 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1022346, whole genome shotgun sequence.
ACCESSION   DBNHOX010000706 DBNHOX010000000
VERSION     DBNHOX010000706.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1022346"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="AC16UZ_15370"
     CDS             complement(<1..444)
                     /locus_tag="AC16UZ_15370"
                     /inference="COORDINATES: protein motif:HMM:NF036581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HIR6248116.1"
                     /translation="MIDSGRAGLDAGMVLDCVSNGIPICRDLADALGQSERAPDYLIY
                     GMAPASGMLSPHERGLVFEAMDRGMNVVSGLHEFLSDDPAFVAASAANNVRIVDVRKP
                     PAPKELRMFSGRIAEVDCPRIAVLGTDCAIGKRTTATALTRALNAH"
     gene            complement(678..1157)
                     /locus_tag="AC16UZ_15375"
     CDS             complement(678..1157)
                     /locus_tag="AC16UZ_15375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6248117.1"
                     /translation="MTGQKSGALLEAAPRPAPPSPVRGSASEAGRGPELSVLIIDRDQ
                     DILAIATSILESCGHRVTALRRVSDGEKRLRTHVDDVVLLDLDMSRAYGSRLLKTALS
                     GPRGGRVILTLTSDRCVESLLDALGAGAWGHLRKPFSATGLLELVGRQGSKRSTPKT"
     gene            complement(1154..1642)
                     /locus_tag="AC16UZ_15380"
     CDS             complement(1154..1642)
                     /locus_tag="AC16UZ_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248118.1"
                     /translation="MLTTHFPAMSLVLLSVAACASPGREQPARINSLDMQLDTRWRGS
                     LASPAGLPDTLPMSGSASMAPAADNGSTVVTVSLAQAAPGGLHPWEARLGRCGMQKDD
                     GVFGSSGEYETMTIDPDGRAMGSVTVSQPMPVSGDYFVAVYASEENRSVVVCGDLAPP
                     SQ"
BASE COUNT          342 a          670 c          623 g          332 t
ORIGIN      
        1 gtgggcgttg agggctcggg tcagcgccgt ggcagtcgtg cgcttcccga tcgcgcagtc
       61 ggtgccgagc acggcaattc gtggacagtc gacctctgca atgcgcccgc tgaacatccg
      121 cagttccttc ggtgcgggcg gcttccgaac gtcgacgatc cgaacgttgt tggcagcgct
      181 cgcggccacg aaggcggggt catcgctcag aaactcgtga agcccgctca cgacgttcat
      241 cccgcggtcc atcgcctcga acaccagacc gcgctcgtga ggcgacaaca tgccgctcgc
      301 cggtgccatc ccgtatatga ggtagtcggg cgctctctcg gactgcccga gcgcgtcggc
      361 gaggtcacgg cagatcggaa taccgttcga cacgcaatcc agcaccatcc ccgcatcgag
      421 gcctgcgcgt ccactgtcga tcacggaaac gacctcgtac ctctccgagt gtcgcacgag
      481 gccgttggcc gtcttgccgt caagggaacc gaaattcgcc tcgcaataga cgaccgccgt
      541 cggagctcgc gcgtcttccc gctcggacgg tgaccgcgta tgggtcgttg cccggcctgc
      601 ctcaccgcgc ccgcgtgctt cgggagactc gctcgaacga aatagtcgcg ggtatccgga
      661 caccgtagtc ctctgactca ggtcttcggc gtcgatcgtt tcgacccctg gcgtcccacc
      721 aactccagga gccccgtcgc ggagaacggc ttccgcagat ggccccatgc gcccgctccc
      781 agcgcatcga gaagcgattc gacgcatcta tcactcgtaa gagtcagtat gaccctgccc
      841 ccccgtggcc cagaaagggc agtcttcaga agcctggatc catacgcgcg cgacatatcg
      901 aggtcgagga gcacgacgtc atctacgtgc gtgcgcaagc gtttctcgcc atcggagact
      961 cgtcgcaggg ccgtgacccg gtggccgcat gactccagga tggacgtggc tatggccagg
     1021 atatcctggt cgcgatcgat gatcagaacg ctgagttcag ggccccgccc tgcttcactc
     1081 gccgagcctc gcacgggcga gggcggcgcc ggtctcgggg ctgcttccag cagagcgccg
     1141 ctcttctgac cggtcactgg gacggtggcg ccaggtcgcc gcaaaccacc accgaccggt
     1201 tctcctccga ggcatagacc gccacgaagt agtccccgct gaccggcatc ggctgtgaca
     1261 cggtgacgct ccccatcgcc cggccgtcag ggtcgatcgt catggtctcg tactcgccgc
     1321 tcgagccgaa gacgccatcg tccttctgca tgccacatcg gcctagacgc gcttcccacg
     1381 ggtgcaagcc accgggggcg gcctgagcga gggacacggt caccacggtg ctaccgttgt
     1441 ccgctgccgg cgccatggat gccgatccgc tcatcggcag cgtgtcgggc aatccggccg
     1501 gagaagccag acttccgcgc caccgggtgt ccaactgcat gtccagcgag ttaatgcggg
     1561 ccggctgctc tcgaccgggg gaagcgcacg cggcgacaga gagcagaacc agggacatcg
     1621 ccggaaagtg agtcgtgagc atgtgaccgt ccgtttcgag atcctcaagc tcccgcgggg
     1681 gatcggcgcc tcgaatcatg cgccggcatg agatagggac tagtccgtaa ggatgagatg
     1741 acgggccgag gcgccaccgg aggcgacctc accgacggga tccgagggcc tgcgactcgt
     1801 ttgggcgcag tcacacgact ccaccgtgtc gggtccagac ctgcggcttc cttgtcgact
     1861 ggagtggtcc cgagccgatg ggcggcacgc tggtacccat caggaaaccc cgcccagtca
     1921 cctaagacca agcggggctt ccgtttcgct cagtgggcct gggtaga
//
LOCUS       DBNHOX010000707         3232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1085581, whole genome shotgun sequence.
ACCESSION   DBNHOX010000707 DBNHOX010000000
VERSION     DBNHOX010000707.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1085581"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1268)
                     /locus_tag="AC16UZ_15385"
     CDS             complement(129..1268)
                     /locus_tag="AC16UZ_15385"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017255227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase family protein"
                     /protein_id="HIR6248119.1"
                     /translation="MTDVRPFRIEIPEKDLDDLGQRLALTRLPEKETPDDWSQGVPLA
                     YMREIVDYWQSDYDWRRAEEEINRFPQFTTELDGLNVHFIHVKSPEPNALPLVMTHGW
                     PGSIVEFLKVIGPLADPASHGGDPADAFHVVCPALPGYGFSGKPITPGWGVEKIGSEW
                     ANLMARLGYESYVAQGGDWGSAVTTAIGMQDPDHCRAIHLNMVVAQPDPATMDDLTDL
                     EKRGIAGMQHYQQWDSGYSKEQSTRPQTIGYSLVDSPVGQAAWIMEKFWAWTDCDGHP
                     ENALSRDEMLDNIMLYWLTASGASSARLYWESFGSFSAGDVSVPTGCSIFPKEIFTCS
                     RRWAEQRFKNIVYWNELDRGGHFAAFEQPELFVGELRNCFRQVRG"
     gene            complement(1295..2065)
                     /locus_tag="AC16UZ_15390"
     CDS             complement(1295..2065)
                     /locus_tag="AC16UZ_15390"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6248120.1"
                     /translation="MSLFRLDNKNIAITGASSGFGHHFAGVLSEAGASVVLGARREDK
                     IRDRVDEINAAGGKATAVKLDVRDADSVRGFLEAAEAAYGPLDVVINNAGVEAGAKTY
                     LMIDEDDWDFVMDTNLKGAWLVSKTYTELVLAAGRKQGNIINISSITDTRTIKGQFPY
                     AVSKGALTRMTEVMALEAAKYGIRVNALAPGYILTDVSRLLLESDQAPEFVKGIPMRR
                     FGEFSDLDGPLLLLASDASTYMTGSVVVVDGGHICASL"
     gene            complement(2138..2398)
                     /locus_tag="AC16UZ_15395"
     CDS             complement(2138..2398)
                     /locus_tag="AC16UZ_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248121.1"
                     /translation="MPDDELKRARDDARCEAAIQTLTDVLWRFEETCEGSYTLYDLVG
                     YLVEDLVREGCCAACIAESVTAAFKDIGADTTEHRRDEGTIH"
     gene            complement(2520..2596)
                     /locus_tag="AC16UZ_15400"
     tRNA            complement(2520..2596)
                     /locus_tag="AC16UZ_15400"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2560..2562),aa:Pro,seq:cgg)
     gene            complement(2651..>3232)
                     /locus_tag="AC16UZ_15405"
     CDS             complement(2651..>3232)
                     /locus_tag="AC16UZ_15405"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6248122.1"
                     /translation="GGFYLAIDATAGEREHGSLEPLLTQPISRGALVLGKVAATAVFS
                     AASLALFLASLAISLPYAPLHKVGMSLHIDVSTALTVFVMALPLILFAAALLNVVASF
                     AKSYKEAQTYLTFVILIPTLPLIITRMMNVEPSAALMMIPSLSQGTLINDAVAGEPVQ
                     WVFLLISVVSTTLTAGALTMLAVYLYKRERILI"
BASE COUNT          548 a         1018 c         1071 g          595 t
ORIGIN      
        1 cgaccaggcg tgcaggtccg catagcccgt cctggagcgg ccggataccc gggcggcttc
       61 ctcggtgaag cgggccatgt tcgattccag cgcccggacc ggatccgggc gccagatggg
      121 cccctcggtc acccgcgtac ctggcggaag cagtttctca gctcgccgac gaacagctca
      181 ggctgctcga aggcggcgaa gtgtccgccc cggtccagct cgttccagta gacgatgttc
      241 ttgaagcgct gctccgccca ccggcgcgag caggtgaaga tctctttcgg gaagatgctg
      301 catcccgtcg gcacgctcac gtcgcctgcg gagaagctgc cgaagctctc ccagtagagc
      361 cgtgcggacg atgcgccgct ggccgtgagc cagtagagca tgatgttgtc gagcatctcg
      421 tcccggctca gggcgttctc cggatgcccg tcgcaatcgg tccacgccca gaacttctcc
      481 atgatccagg cggcctggcc cacaggtgag tcgaccaggc tgtagccgat ggtctgggga
      541 cgcgtgctct gctccttgga gtagccggag tcccactgct ggtagtgctg catgcccgct
      601 atgcctcttt tctcgaggtc ggtcaggtcg tccatggttg ccggatccgg ctgggcgacc
      661 accatgttga ggtgaatagc gcggcagtgg tcgggatcct gcatcccgat agcggtggtg
      721 actgccgagc cccagtcgcc cccctgggcg acgtagcttt cgtagcccag gcgggccatt
      781 aggtttgccc actcgctgcc gatcttctcc accccccagc ccggggtgat gggcttgccc
      841 gaaaagccgt agccggggag cgccgggcag accacgtgaa aggcgtctgc cggatcgccg
      901 ccgtgactgg cgggatcggc caacgggccg atcactttca ggaactcgac gatggagccg
      961 ggccagccgt gggtcattac caggggcagg gcgttcggct cgggcgattt cacgtggatg
     1021 aagtggacgt tcagcccgtc gagctcggtg gtgaactgcg ggaagcgatt gatctcttcc
     1081 tcggcgcgtc gccagtcgta atcggactgc cagtagtcga cgatctcgcg catgtacgcc
     1141 agcggcactc cctggctcca gtcgtcgggc gtttccttct cgggcagccg ggtcagcgcc
     1201 aggcgctggc ccaggtcgtc cagatccttc tcgggtatct cgatcctgaa ggggcgtacg
     1261 tcggtcatgg tctgtctcct gcctggtttc agcgtcacag cgacgcgcag atgtggccgc
     1321 cgtccacgac gaccacggat cccgtcatgt aggtcgaagc gtcggaggcc agcagcaaca
     1381 ggggaccgtc caggtccgag aactcgccga accggcgcat gggaatgccc ttcacgaact
     1441 cgggcgcctg atcgctctcg agcagcaggc ggctcacgtc cgtgaggatg tagccgggcg
     1501 cgagcgcgtt gacgcgtatc ccgtacttgg ccgcctccag ggccatgacc tcggtcatgc
     1561 gggtgagcgc tcccttggag acggcgtacg gaaactgtcc ctttatcgtg cgggtatcgg
     1621 taatggatga gatgttgatg atgttgccct gcttgcgacc ggccgcgagg acgagctcgg
     1681 tgtaggtctt ggagacgagc cacgcacctt tgaggttcgt atccatgacg aagtcccagt
     1741 catcctcgtc gatcatcaga taggttttcg cgccagcctc gacgccggcg ttgttgatga
     1801 ccacgtccag gggcccgtag gcagcctcgg cggcctcgag gaagcctctg acgctgtcgg
     1861 cgtctcgcac gtccagcttt accgcggttg ccttgccgcc ggcagcgttg atctcgtcga
     1921 cccggtcgcg aatcttgtct tcgcggcggg cgccgagcac cacgctggcc ccggcctcgg
     1981 aaagcacgcc tgcgaagtgg tggccgaatc ccgaggatgc gccagtgatg gcgatgttct
     2041 tgttgtcgag ccggaacaga ctcacgcgaa aaccctcctg ccctgacggg agcacaatcc
     2101 tagcgacaaa gaatgtggca ggccaggcag gcggtggtca gtgtatggtt ccctcgtcgc
     2161 gccggtgctc ggtggtatcg gcgccgatgt ccttgaacgc cgccgtcacg gactccgcga
     2221 tgcacgcggc gcagcagccc tcccggacca ggtcttccac caggtagccc acgaggtcgt
     2281 agagggtgta ggagccctcg caggtttcct cgaaccgcca cagcacgtct gtgagggtct
     2341 ggattgccgc ctcgcagcgg gcgtcgtcgc gcgcgcgttt gagttcgtcg tccggcatgt
     2401 cttgggctcc aggcatgatg cggtatcggg cattgtaacc ggccggcgcg gggcttgcgt
     2461 cgcgctttga gcgggacgcg tcggtggtgc gtcggtagcc gcaaaccact gagtcaaaat
     2521 ggtcggagtg agaggatttg aacctccggc ccccacgtcc cgaacgtggt gctctaccaa
     2581 gctgagctac actccgactg cccctggtga gggccgcgta ttgtaaaagc gccccggcgc
     2641 gggaacaaca tcagatcaat atgcgttcgc gcttgtaaag gtacacggcc agcatcgtca
     2701 gcgccccggc cgtcagcgtc gtcgagacca cggatatcag cagaaaaacc cattgcacgg
     2761 gttcacccgc caccgcgtcg ttgatcaacg tcccctggct caggctcgga atcatcatca
     2821 gggccgcgga aggctccaca ttcatcattc gcgtgatgat caggggaagc gtggggatga
     2881 ggatgacgaa cgtcaggtaa gtctgcgctt ccttgtagct cttcgcgaac gaagcgacca
     2941 cgttcagcag ggcggccgcg aacagtatca gcggcagcgc catgacgaaa acggtcaacg
     3001 cggtcgagac atcgatgtgc agcgacatgc ccaccttgtg gaggggcgcg tagggcagag
     3061 agatggccag gcttgcgagg aacaacgcca gggaggctgc gctgaacacc gccgtggcgg
     3121 ctaccttgcc cagcacgagg gcgccgcgcg atatgggctg ggtcagcaac ggctccaggg
     3181 agccgtgctc gcgctcgccg gccgttgcgt cgatggccag gtaaaagccg cc
//
LOCUS       DBNHOX010000708         3844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_957924, whole genome shotgun sequence.
ACCESSION   DBNHOX010000708 DBNHOX010000000
VERSION     DBNHOX010000708.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_957924"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="AC16UZ_15410"
     CDS             complement(<1..750)
                     /locus_tag="AC16UZ_15410"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR6248123.1"
                     /translation="MKRVDRYLVGVLLLGAVPVLLLLLGLFGFVTLAEELEDVGKGQY
                     EEADAIRVALLTLPRIAIDLLPVAVLLGGVMALGNLAQEQELTALRAVGLSQWRMAYP
                     LALAAIVLSYVVLLAQQFVVPRFEEQAKELQAKSLVNTMRGGETTEFWTRDGTHFIRV
                     GDVRFGMIPADVEIYDLGDRGVVQRVLQAEYADVIGPGQWLLHGVEERRILGKEVRRT
                     GRDTYEWESFLSPDQMASLIAPMETLTLGDLY"
     gene            complement(747..1817)
                     /gene="lptF"
                     /locus_tag="AC16UZ_15415"
     CDS             complement(747..1817)
                     /gene="lptF"
                     /locus_tag="AC16UZ_15415"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HIR6248124.1"
                     /translation="MIINRYLVRELLLPFFAVGGVLTVIFTTYSMTRFLDKAADGLLR
                     TSEVLQLTALKSFIAQEVLLPVSLFVALVLALGRLYSDWEMTALRSFGFAERQVLFPI
                     MSVALGFALVVAVFSLVGRPWAYEHLYRLEVEAEATSELERIKPGRFYLYDDNARTVF
                     IEELKAEDDEIQGLFVRALDGDDLEIISAPSGTVSEYVSDKNHQVDLRDAQVYRKTAQ
                     GADFLGIFGELRIFVPVAAVQPVGYKPKSQNSFVLRESDALVDQAEYQWRLSAPVSTI
                     LLALLAVLLGRSRPRQGRFARLTVAVVIYAVYFNVLGIGRTWVEQGTAATLWWVPATF
                     AGGIVLAYVPWARWFRRRGRSA"
     gene            1907..2416
                     /locus_tag="AC16UZ_15420"
     CDS             1907..2416
                     /locus_tag="AC16UZ_15420"
                     /inference="COORDINATES: protein motif:HMM:NF015901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HIR6248125.1"
                     /translation="MLKRFFAAELASAIALALVQLSCSAWAGELPALDDQDTVVVTAD
                     QAWESEDGDSLSFAGNFNLNAPDFHVASDTAVIHGPLEDPNRIVAEGSPVRFWVADPD
                     SGNRTHGTGRRLEYDRQRDLVRLEGDAVLEDDRTVMRSSELEYNTATKRLVAQGSGGV
                     EIVTNPNRD"
     gene            complement(2426..2854)
                     /locus_tag="AC16UZ_15425"
     CDS             complement(2426..2854)
                     /locus_tag="AC16UZ_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR6248126.1"
                     /translation="MNATAEKFGYPDTLVREYGHWLVLVRPQQVSLGSLVLVCKEPVT
                     RFSEVSRAAFTELHDVIAAAEGALSSLYSYDRINYLMLMMVDPDVHYHVIPRYEAARL
                     LGTAEIADHGWPGPPALGEFHELDAAEREALLGQLREAWS"
     gene            complement(2856..3608)
                     /locus_tag="AC16UZ_15430"
     CDS             complement(2856..3608)
                     /locus_tag="AC16UZ_15430"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HIR6248127.1"
                     /translation="MKAIILSAGQGRRLLPLTAGTPKCGVEIAGQSLLEWQLGEVAQC
                     GIDEVVVVTGFHADIVDDLVSRCSMVPVRTLHNPFFDASDNLGTCWVARSEMTDPFVI
                     INGDTLFEAAILQKLLASPANTPVTLVVDRKDHYDEDDMKVVVSDGRLRRVGKRLELD
                     DVNGESIGMMAFRGLGPAKFVSKVESLMRSDMGLKLWYLSAVDQLAQSGEVGVCDIHG
                     LSWCEVDDRDDLDNAAGVVTAWRKPASRAAAD"
     gene            complement(3661..>3844)
                     /locus_tag="AC16UZ_15435"
     CDS             complement(3661..>3844)
                     /locus_tag="AC16UZ_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017338059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HIR6248128.1"
                     /translation="SADPRHGVVDGSGRVHGYGNLFVAGSSVFATAGWANPTLTILAL
                     ALRLGDHLHGLLGGKS"
BASE COUNT          758 a         1290 c         1173 g          623 t
ORIGIN      
        1 gtagaggtcg cccagggtca gggtttccat cggcgcgatg agggaggcca tctggtccgg
       61 agacaggaag ctctcccact cgtaggtgtc acggccggtg cgcctgacct ctttgccgag
      121 aattcggcgc tcttcgacac cgtgaagcag ccactggccc ggcccgatca cgtccgcgta
      181 ctcggcctgc agcacgcgct gcacgacgcc gcgatcgccg agatcgtaga tctcgacatc
      241 agcgggaatc atgccgaaac ggacgtcccc caccctgatg aagtgagtgc cgtcgcgggt
      301 ccagaactcg gtggtctcgc cgccacgcat cgtgttgacc agcgacttcg cctgaagctc
      361 cttggcctgt tcctcgaacc gaggcaccac gaactgctgc gccagcagca cgacgtaaga
      421 caacacgatg gccgccagcg caagcggata ggccatccgc cattgggata gaccaaccgc
      481 tcgcagcgcc gtcagctcct gttcctgagc caggttgccg agcgccatca ccccaccgag
      541 cagcaccgcc accgggagaa gatcgatggc gattcgcgga agcgtcagca gggcgacgcg
      601 gatcgcgtcc gcttcctcgt actgcccctt gccgacgtcc tccagctctt cggcaagggt
      661 gacgaaaccg aacagcccca gcagcagcaa cagaacgggt accgcgccca gcagcaagac
      721 gcctacaagg tagcggtcca cacgcttcac gcgcttcgcc cccgtcgtct gaaccaccgg
      781 gcccatggca cgtaggccag cacgatgccg cctgcaaagg tcgccgggac ccaccacaac
      841 gtcgccgccg tgccctgctc gacccaggtc cttccgatgc ccagaacgtt gaagtacacg
      901 gcgtagatga ccacggcaac cgtgagccgg gcaaagcgcc cctgacgggg ccgggaccgt
      961 ccgagcagga cggccagcaa cgccagcaga atggtcgaaa cgggcgccga caggcgccat
     1021 tggtactcgg cctgatccac tagcgcgtcg gattctctga ggacgaacga gttctgggat
     1081 ttcggcttgt agcccacggg ctgcaccgcc gccaccggaa cgaatatgcg cagctcgccg
     1141 aagatgccca gaaagtcggc gccctgcgca gttttcctgt agacctgagc atcgcgaagg
     1201 tcaacctggt ggttcttgtc gctgacatac tcgctcacgg taccgctggg cgcggagatg
     1261 atttccagat catcgccgtc cagcgcgcgc acgaacagcc cctgaatctc gtcgtcttcg
     1321 gccttcagct cctcgatgaa gacggtccgg gcattgtcgt cgtacaggta gaatcgcccg
     1381 ggtttgatgc gctcgagctc cgaggtcgct tccgcttcga cctccagcct gtagaggtgc
     1441 tcgtacgccc agggcctgcc caccagggag aaaacggcga cgacgagcgc aaagcccagg
     1501 gcgacgctca tgatcgggaa aagcacctga cgctcggcaa acccgaacga gcgcagggcc
     1561 gtcatctccc agtcgctgta gagtcgcccg agggcaagca ccagggcgac gaagaggctg
     1621 accggcagca gcacttcctg agcgatgaaa gacttgagcg ccgtaagctg cagcacctcc
     1681 gacgtccgca gcaaaccgtc ggcagccttg tcgaggaacc gggtcatgct gtaggtggtg
     1741 aatatcaccg tcaacacgcc gccaaccgcg aaaaacggca gcagaagctc gcgaacgagg
     1801 taccggttga tgatcagtgc tctaaccctc tcccgaactg caggccagcc cgtcgccgga
     1861 tcctgatatc atcgacccgg tttcccgacg accccggccg agctccatgc tgaaaagatt
     1921 tttcgccgcg gaactggctt cggccattgc gctggcgttg gtgcagctct cttgctcggc
     1981 atgggccggc gagctgcccg cgctggacga tcaagatacc gtagttgtca ccgccgacca
     2041 agcttgggag tccgaggacg gcgactcgtt gagctttgcc ggcaacttca acctcaacgc
     2101 ccccgacttc cacgtcgcat ccgacacggc ggtcattcac gggcctttgg aggatcccaa
     2161 ccgcatcgtc gcggagggca gccccgtgcg cttctgggtt gccgatcccg attccgggaa
     2221 caggactcac ggaaccggca ggcggctgga atacgatcgg caaagggatc tggtccggct
     2281 cgagggggat gccgtactgg aagacgaccg gaccgtgatg cgaagttccg agctcgaata
     2341 caacaccgcc acgaaacgac tcgtcgcgca aggatcgggc ggcgtggaga tcgttacgaa
     2401 cccgaaccgc gactgagcac ccggctcagc tccaggcctc acgcaactgc ccgagcaggg
     2461 cttcacgttc ggccgcgtcg agctcatgaa actcccccag ggccggcggt ccgggccagc
     2521 cgtggtcggc tatctccgcc gtccccagca gacgggcggc ctcgtagcgg ggaatgacgt
     2581 gatagtgcac gtcggggtcg accatcatca gcatcaggta gttgatcctg tcgtagctgt
     2641 acaggctcga cagggcccct tcggccgcgg ctatcacgtc gtgaagctcg gtgaacgccg
     2701 ccctgctgac ctcggagaat cgggtgacag gctctttgca gaccaggacc agggagccga
     2761 gagagacctg ctgcggccgg acgagaacca gccagtgacc gtactcgcgc accagcgtgt
     2821 ccggatagcc gaatttctct gccgtggcgt tcaagtcaat ccgccgccgc ccgcgaggcg
     2881 ggcttgcgcc aggcagtgac cacgcccgcc gcgttgtcga gatcgtcccg gtcgtcgact
     2941 tcgcaccatg acagaccgtg aatgtcgcaa accccaacct cgccgctctg agcgagctga
     3001 tcaacggcag agaggtacca gagcttcaaa cccatgtccg aacgcatcag cgactccacc
     3061 ttgctcacga acttggcagg acccagaccc ctgaatgcca tcatgccgat ggactcaccg
     3121 ttcacgtcgt ccagctcgag tcgcttgccc acacgccgaa gacgcccgtc gctgaccacg
     3181 actttcatgt cgtcctcgtc gtagtgatcc ttacggtcca ccacgagcgt aacgggcgta
     3241 ttcgcgggag atgccagcag cttctgcagg atggccgcct cgaacagcgt gtcgccgttg
     3301 atgatgacga aggggtcggt catttcgctg cgcgcgaccc agcaggtgcc aagattgtcg
     3361 ctcgcatcga agaaggggtt gtgcagggtt ctcaccggga ccatgctgca gcggctgacc
     3421 aggtcatcga cgatgtcggc atggaaaccg gtaacgacca cgacctcgtc gatgccgcat
     3481 tgagccacct cgccgagctg ccattccagc aggctctggc cggcgatctc gacgccgcat
     3541 ttgggcgtcc cggccgtcag cggcagcagc ctgcggccct gaccagcgct cagaatgatg
     3601 gccttcacta gggtaccggt atcgggaaaa cgcgaagggt cggggttttc accgcgaggg
     3661 tcaagacttt ccgcccagca ggccgtgcag atggtctccc agccgcagcg ccagggccag
     3721 aatcgtcagg gtggggttcg cccagccggc ggtggcaaag acggagctgc cggcgacgaa
     3781 cagattgccg tagccgtgaa cgcgcccact cccgtccacc acaccgtgcc gaggatcggc
     3841 actc
//
LOCUS       DBNHOX010000709         2032 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_187278, whole genome shotgun sequence.
ACCESSION   DBNHOX010000709 DBNHOX010000000
VERSION     DBNHOX010000709.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_187278"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2032
                     /gene="rpoB"
                     /locus_tag="AC16UZ_15440"
     CDS             <1..>2032
                     /gene="rpoB"
                     /locus_tag="AC16UZ_15440"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR6248129.1"
                     /translation="LGREDITGEGILTNEDIIDVLRTLIDIRNGRGSVDDIDHLGNRR
                     VRSVGEMAENQFRVGLIRVERAVKERLSLAESEGLMPQDMINAKPVAAAVKEFFGSSQ
                     LSQFMDQNNPLSEVTHKRRVSALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEGPN
                     IGLINSLATYARTNEYGFLETPYRRVKGGKVAQEIEYLSAIDEAQYVIAQSSSPLDAT
                     GAFEGDLVDVRHQNEFTKTSAENVNYMDVSPKQVVSVAASLIPFLEHDDANRALMGSN
                     MQRQAVPTIRTEKPLVGTGMERHVARDSGVCVIASRGGTVDSVDAGRIVVRVADAETE
                     AGEAGVDIYNLTKFTRSNQNTCINQRPLVKPGDVIARGDILADGPSCDMGELALGQNM
                     RIAFMPWNGYNYEDSILVSERVVQEDRFTSVHIQELTCVARDTKLGPEEITCDIPNVG
                     EGALSKLDESGIVYIGAEVGAGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDT
                     SLRVPTSVKGTVIDVRVFTRDGVEKDQRAKDIEQSELAQHRKDLNDEYRIVETATYER
                     LRRALLGKKAASAPGKSKGGTVDEALLDGLGNDDWFKIRMADDALNAQLERAEAQLME
                     RKARLDAVYADKRSKLESGDDLAPGVLKIVKVYLAIKRHIQPGDKMAGRHGNK"
BASE COUNT          419 a          625 c          667 g          321 t
ORIGIN      
        1 gctcggccgt gaagacatca ccggcgaagg aatcctcacc aacgaggaca tcatcgacgt
       61 gctccgtacc ctcatcgaca tccgtaacgg tagagggtct gtggacgaca tcgaccatct
      121 cggcaaccga cgggtccggt ccgtgggcga gatggcggag aaccagtttc gcgttggcct
      181 cattcgagtg gagcgcgcgg tcaaggagcg gctgagcctg gcagagtccg agggcctgat
      241 gcctcaggac atgatcaatg ccaagccggt ggcggcggcg gtcaaggagt tcttcggttc
      301 cagccagctg tcgcagttca tggatcagaa caacccgctg tccgaggtga ctcacaagcg
      361 ccgcgtttcg gcgcttggcc cggggggcct gacgcgcgag cgtgcaggct tcgaggtgcg
      421 cgacgtccac ccgacccatt acgggcgcgt ctgcccaatc gagacgccgg aaggcccgaa
      481 catcggcctc atcaattcgc tggcgactta cgcccgcacg aacgagtacg gtttcctcga
      541 gacgccctac cgccgggtca agggcggcaa ggtcgctcaa gagatcgagt acctgtcggc
      601 catcgacgag gcgcagtacg tgatcgccca gtcgagttct cctctggacg cgacgggtgc
      661 gttcgagggc gatctcgtcg acgtgcgcca tcagaacgag ttcaccaaga cttcggcgga
      721 aaacgtgaac tacatggacg tctcgcccaa gcaggtcgta tccgtggcgg cctcgctgat
      781 tccgtttctg gagcacgatg atgccaaccg ggccctcatg ggctcgaaca tgcagcgtca
      841 ggcggttccg accattcgca ccgaaaagcc gctggtcgga acggggatgg aacggcacgt
      901 ggccagagat tccggcgtct gcgtcatcgc cagccgcggg ggcaccgtgg acagcgtgga
      961 cgcgggccgc atcgtggtgc gcgtggccga cgcggagacc gaggcgggtg aagccggggt
     1021 cgacatctac aacctgacca agttcacccg atccaaccag aacacctgca tcaaccagcg
     1081 tccgctggtg aagccgggag acgtgatcgc ccgcggagac attctcgcgg acggcccgtc
     1141 gtgcgatatg ggtgagctgg ctctgggcca gaacatgcgc atcgcgttca tgccctggaa
     1201 cggatacaac tacgaggact ccatcctcgt gtccgaaagg gttgtccagg aggaccgctt
     1261 caccagcgtc cacatccagg aactcacctg tgttgcccgg gataccaaac tcggacccga
     1321 ggagattacc tgcgacattc ccaacgtcgg cgagggtgcg ctctccaagc tggacgagtc
     1381 ggggatcgtc tacatcggcg ccgaggttgg tgccggcgac attctcgtcg gcaaggtcac
     1441 cccgaagggc gagacccagc tgacccctga agagaagctg ctgcgtgcca tcttcggcga
     1501 gaaagcatcg gacgtgaaag acacgtcgct tcgagtcccg acgagcgtca agggcaccgt
     1561 catcgacgtc cgggtattca cccgggacgg ggtggagaag gatcagcggg ccaaggacat
     1621 cgagcagtcc gagcttgcgc agcaccgcaa ggatctcaac gatgagtacc gcattgtcga
     1681 gaccgcgacc tacgagcgct tgcggcgggc tctgctcggc aagaaggcgg ctagcgctcc
     1741 gggcaagagc aagggcggca cggtggacga agcgctgctc gacggtctcg gcaacgacga
     1801 ctggttcaag atccggatgg ctgatgacgc cctgaacgcc cagctcgagc gagccgaggc
     1861 gcagctcatg gagcgcaagg cccgtctgga tgccgtctat gcagacaagc gctccaagct
     1921 cgaaagcggc gacgacctgg ctcccggcgt gctcaagatc gtgaaggtgt atctggcgat
     1981 caagcgacac attcagcccg gcgacaagat ggccggcagg cacgggaaca aa
//
LOCUS       DBNHOX010000710         1995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1408016, whole genome shotgun sequence.
ACCESSION   DBNHOX010000710 DBNHOX010000000
VERSION     DBNHOX010000710.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1408016"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="AC16UZ_15445"
     CDS             <1..390
                     /locus_tag="AC16UZ_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248130.1"
                     /translation="RGLWVFGVVQLITILGFAYLAQVGNDLVVLAVVISAEYLGVGLG
                     TAAFVAFIARETSPALAATQFALFSAVTALPRTLTAAATGWLVDVVGWVEFFYLCTLA
                     AVPGMVLLVWVAPFTAPSNEPQPPGTR"
     gene            533..988
                     /locus_tag="AC16UZ_15450"
     CDS             533..988
                     /locus_tag="AC16UZ_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HIR6248131.1"
                     /translation="MGLWFLVFFLTPILEMYLLIEVGGYIGTLPTIALVMLTAVVGVA
                     LLRIQGVETLTRGVGRLQRGEVPATEMAEGILLAVAGALLITPGFVTDAIGFVLLFPP
                     SRTGIARRMLSRLTMVGPDGRPFSPGEPGPGPTVIEGEFERRPPDDGQP"
     gene            1118..1408
                     /locus_tag="AC16UZ_15455"
     CDS             1118..1408
                     /locus_tag="AC16UZ_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020799908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR6248132.1"
                     /translation="MNIRPLHDRVVVRRLEEESTSAGGIVLPDSAAEKPSQGEVLAIG
                     SGKLLDNGDVRALDVKVGDRVLFGQYAGSTVKIDGEELLVLSESEIFGVLEG"
     gene            1435..>1995
                     /locus_tag="AC16UZ_15460"
     CDS             1435..>1995
                     /locus_tag="AC16UZ_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HIR6248133.1"
                     /translation="MSAKDVVFGDEARSRMLEGVNVLAEAVKVTLGPKGRNVVLDKSF
                     GAPTVTKDGVSVAKEIELKDRFENMGAQMVKEVASQTSDEAGDGTTTATVLAQSILTE
                     GLKSVAAGMNPMDLKRGIDKAVASAVIELEKMATPCEDSKSIAQVGTISANSDEPIGQ
                     IIAEAMEKVGKEGVITVEEGSGLENEL"
BASE COUNT          353 a          594 c          669 g          379 t
ORIGIN      
        1 cggggcttgt gggtgttcgg cgtggtgcag ctgatcacca tcctgggctt cgcctatctg
       61 gcccaggtgg gcaatgacct tgtggtgctc gcggtggtga tctccgccga gtacctgggg
      121 gtagggctcg ggacggccgc cttcgtggct ttcatcgccc gtgagacttc gcccgctctg
      181 gcggccaccc agtttgccct gttcagcgcg gtaaccgcgc tgccgcgaac gctgaccgcc
      241 gcggcgaccg gctggctggt ggatgtggtc ggctgggtcg agttcttcta tctgtgcacc
      301 ctggcagcgg ttccggggat ggtgctgctg gtgtgggtcg cgcccttcac ggctcccagc
      361 aatgagccgc agccacccgg gacccgctga acgagacccg ctcggccttc aggcgtgcca
      421 tctggcctgc gggatcatcg ccaccacctg ccacacccgc tgcctcggtg acggttgact
      481 atccgcgagc gtgccctcag attgagccga cctcgtctgg tcacccgcca tcatgggatt
      541 gtggttcctc gtcttcttcc tcacccccat cctggaaatg tacctgctca tcgaggtggg
      601 cggctacatc gggacgctgc cgaccatcgc tctggtcatg ctgactgcgg tggtcggcgt
      661 tgcgctgctg cgcattcagg gagtagagac gctgacccgg ggggttggtc ggctgcaacg
      721 cggcgaggtg cctgcgacgg agatggcaga aggaattctt ctggccgtgg cgggggccct
      781 gctcatcacc ccgggtttcg tgaccgatgc catcgggttc gttctgctct tcccgccgag
      841 ccgcacgggt atcgcacggc gcatgctctc caggctcacc atggtcgggc ccgacggtcg
      901 ccctttctcg cccggagagc cgggtcccgg gcccacggtc atcgagggag agttcgagcg
      961 gcgcccgccc gacgacggcc agccgtaaaa cgtttacttc ccaggacttg aatctctccg
     1021 ggccgccact attatatgcg gcccttggat tagcactctg agcgttagag tgctaacgat
     1081 tacccgatag ggactttaac tgggagaaac acaggtaatg aacattcgac ccttacacga
     1141 tcgcgttgtc gtgcgccggt tggaagagga aagcacctcg gcaggcggca tcgtgctgcc
     1201 cgactctgcg gcagagaagc cttctcaggg cgaggtgctg gccataggtt ccggcaagtt
     1261 gcttgataac ggtgacgtcc gtgcgttgga cgtcaaggta ggcgaccgag ttcttttcgg
     1321 ccagtacgcc ggcagcacgg tgaagatcga cggtgaggaa ctgcttgtcc tcagcgagag
     1381 cgagatcttc ggcgtcctgg aaggctaacc agcgagattt atgaggaaaa agcgatgagc
     1441 gctaaagacg tagtttttgg tgatgaagcc cggagccgga tgctcgaggg cgtgaatgtt
     1501 ctggccgaag cggtcaaagt cacgctgggc ccgaaggggc gcaacgtggt gctggacaag
     1561 tcgttcggcg cgccgaccgt gaccaaggac ggtgtgtccg tggccaagga aatcgagctg
     1621 aaggacaggt tcgaaaacat gggcgcgcag atggtgaagg aagtcgccag ccagacctcc
     1681 gacgaggccg gtgacggcac cacgacggca acggtgctgg cccagtccat cctgacggaa
     1741 ggtctcaagt ccgttgcggc cggcatgaac ccgatggatc tcaagcgggg tatcgacaag
     1801 gccgtggcca gtgccgtgat cgagctcgag aagatggcca cgccgtgcga agactccaag
     1861 tccatcgccc aggtcgggac catctccgcc aacagcgacg agcccattgg ccagatcatc
     1921 gctgaggcca tggagaaggt cggcaaggaa ggcgtcatca ctgtcgagga aggcagcggc
     1981 ctggagaacg agctg
//
LOCUS       DBNHOX010000711         1890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_379431, whole genome shotgun sequence.
ACCESSION   DBNHOX010000711 DBNHOX010000000
VERSION     DBNHOX010000711.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_379431"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="AC16UZ_15465"
     CDS             <1..1050
                     /locus_tag="AC16UZ_15465"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6248134.1"
                     /translation="NGFFYVIDRTTGKVVSAEKFVPVNWAQRVDLQTGRPVEAPGARF
                     VDAPFTAVVGGAGAHNWQPMSYSPGTGLVYIPAQEVPFVYTQEEGFAFKPGEWNLGVV
                     LAGARLPADEATRREIVAANQGRLIAWDPVNQREAWRVEHGAPWNGGTLATAGNLVFQ
                     GLTDRTFRAYRATDGKQLWSFDTQNVIITAPMSFAVNGEQHVAVMAGNGGGLPLTLPT
                     FSGPLQTPNGRLLVFKLGGTAELPPFEPEPLKPVIADVALTPDELDTGFDLFGRYCAA
                     CHGLETLSAGVLPDLRSSPALASSDAWRSIVHDGALETRGMIGFQARMSADQVEIIRG
                     YVANEARKLESDADG"
     gene            1069..>1890
                     /locus_tag="AC16UZ_15470"
     CDS             1069..>1890
                     /locus_tag="AC16UZ_15470"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014869595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR6248135.1"
                     /translation="MTPTERDLWLLPLGGCGEIGMNLNLYGHDGRWLMVDCGITFAEP
                     GESGPHIQMADPAFIASRRQRLCALIVTHAHEDHVGAVAHLWRQLQCPVYATPFTAEV
                     LRRKLAEARLLDDVPIHVVRSGDRLTLEPFDVEWVALTHSIPEPQALVIRTPAGSVFH
                     TGDWKLDPSPVIGADYDEARCRQLADEPIGTMVCDSTNAMVEGRSISEGELYAGLLDL
                     VSGARGRVIVGCFGSNVARLHTLIRVAADTGRFACLLGRSLHNYYAAARASGVWES"
BASE COUNT          303 a          652 c          637 g          298 t
ORIGIN      
        1 aacggctttt tctacgtgat cgatcgcaca accggcaagg tcgtctccgc ggaaaaattc
       61 gttcccgtga actgggcgca acgggtggac cttcagaccg gccggccggt ggaagcgccg
      121 ggcgcccggt tcgtcgacgc gccctttacc gcagtggtcg gcggggcggg cgcccacaac
      181 tggcaaccca tgtcttacag ccccggcacc gggctcgtct acatcccggc ccaggaggtg
      241 cccttcgttt acacccagga agaaggcttc gcgttcaagc ccggcgagtg gaacctgggc
      301 gtcgtgctcg cgggcgctcg cctgcccgcg gacgaggcaa cgcggcggga gatcgtggcg
      361 gccaaccagg gccggctcat cgcctgggac ccggtcaacc agcgggaggc ctggcgagtc
      421 gagcacggag ccccgtggaa tggtggcacg ctggccacgg ccggcaacct ggtctttcag
      481 gggctgacgg atcgcacgtt ccgcgcatac cgggctacgg acgggaagca gctctggtcg
      541 ttcgacaccc agaacgtgat catcaccgcg cccatgagct ttgccgtcaa cggagagcag
      601 cacgtggccg tgatggccgg aaacggggga gggctgccgc tgacgctccc cacgttcagc
      661 ggcccgctcc agaccccgaa cgggcggctg ctggtattca agctgggagg cacggccgag
      721 ctgccgccct tcgagcccga gccgctcaag cccgtcatcg ccgacgtggc gctgacgccg
      781 gacgagctcg acaccggctt cgacctcttc gggcgttact gcgccgcctg tcacggtctc
      841 gagaccctga gcgcgggcgt cctgcccgac cttcgctcgt cgcccgctct ggcatcatcc
      901 gacgcctggc gctcgatcgt ccacgacggc gcgctggaga cgagaggcat gatcggcttc
      961 caggcgcgga tgagcgccga tcaggtcgag atcatccggg gctacgtggc caacgaggcc
     1021 cgcaagctgg aaagcgatgc cgacgggtag acgccggctc gacaagccgt gacgcccacc
     1081 gagcgggatc tctggctcct tccgctgggc ggatgcggcg agatcggcat gaacctcaat
     1141 ctgtacggcc acgatgggcg ctggctcatg gtggattgcg gcatcacctt cgccgagccc
     1201 ggggagtccg gaccccacat tcagatggcg gacccggctt tcatcgcctc gcgccggcaa
     1261 cgactctgcg cgttgatcgt cacccacgcc cacgaggatc acgtcggcgc ggttgcccac
     1321 ctctggcgcc agcttcaatg tccggtctac gcaacgccgt tcactgccga ggtgctgcgt
     1381 cgcaagctcg ccgaggcccg gcttctggac gacgtgccca ttcacgtggt gagaagcggc
     1441 gaccgtctga ccctggagcc cttcgacgtg gagtgggtgg ctctgacaca ttcgatcccg
     1501 gagccccagg cgctggtcat cagaaccccg gcgggtagcg tgttccacac cggcgactgg
     1561 aagctggatc cctcgcccgt gatcggggcg gactacgacg aggcgcgctg ccggcagctg
     1621 gccgacgagc cgatcggcac catggtctgc gactccacca acgccatggt ggaagggcgt
     1681 tccatcagcg agggagagct ctacgccggg ctgctggatc tggtcagcgg tgccagaggc
     1741 cgggtgatcg tcggctgctt cggcagcaac gtcgcccggc tgcacacgct gattcgcgta
     1801 gccgccgata ccggccgctt cgcctgcctg ctcggcagaa gcctgcacaa ctactacgcc
     1861 gccgcccggg cgtccggcgt gtgggaatcc
//
LOCUS       DBNHOX010000712         5365 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1408048, whole genome shotgun sequence.
ACCESSION   DBNHOX010000712 DBNHOX010000000
VERSION     DBNHOX010000712.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1408048"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="AC16UZ_15475"
     CDS             <1..342
                     /locus_tag="AC16UZ_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248136.1"
                     /translation="FWISAVDPASPCALVTAIPDLPARRPNNYLAFHEGSLALVIENA
                     GKRLQFHRGPDDACLGRVCEPLAHLVRRRRRVEVELINDVPARSSPYLEPLGRFFHAV
                     TDHKQLILMIG"
     gene            392..1972
                     /locus_tag="AC16UZ_15480"
     CDS             392..1972
                     /locus_tag="AC16UZ_15480"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR6248137.1"
                     /translation="MIVCALIKDEQLRDRLRRLGARAGWRVLFAADDRDLLVTLISGK
                     ATPDVIVSDEPDNLTGVGALPTTRIYLRPDRGEPGTDAFDPALDDQALLQHINNCVNA
                     RRFRARFAAIDRNEPITSLPRHEELFDALSRHVDEPVGMMVVKLDHAEHLYGSLDPVA
                     KTDLLAALSQRLESKLPTQARLGFYDATCFVVTLPRIQSSELERLGNDLRLAVSAPLI
                     DAGGEIHLTISIGCSFASSFNGADHLWASAWAAANAASQAGGDRVLGANPDTAISERI
                     PTALERDEFSLLLQPQWSLQDNRLTAVEALLRWQGRDIGGLAPSHFIPLVEQQGQMAR
                     VGDWVLERACRETAAWLPRMVQPILLTVNVSPQQFHGRSIVTQIARNSAEHRLNPASL
                     ELELSQRNMLRLVDDYREQMYTLRDWGVRFAIDNLGGGLIEPEKLLRCPVDTLKIDRA
                     VVSALSEDTQAADLAAQICELGARFNLRVVGVGVETEEQLKLLAEWGCTDAQGLLVSR
                     PVPVTGIQHLMNRLADLN"
     gene            2013..2930
                     /locus_tag="AC16UZ_15485"
     CDS             2013..2930
                     /locus_tag="AC16UZ_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR6248138.1"
                     /translation="MLLITHRDCLLHEMQHRHPERPDRLRAVLQLLESSGLSRDLKHA
                     EALPASQEALRAVHDPDYLRTLSESVPESGLLPVDPDTAMGPSSLRAAALAAGAVADG
                     VRAVLEGDQSTVFCAVRPPGHHAEHDAAMGFCFYNSIALGAVTALEDPRVERVAVLDF
                     DVHHGNGTVDIFKDRPEVLVCSSFQHPFYPHRYADLARPNIVNTPLPAGTAGADFRRA
                     VEAQWLPAIDNHQPQLILVSAGFDAHRLDPLAQLELCEDDFRWITRLIVASAKTHANG
                     RIVSALEGGYDLAALAASAHAHIEELLAA"
     gene            complement(2934..3716)
                     /locus_tag="AC16UZ_15490"
     CDS             complement(2934..3716)
                     /locus_tag="AC16UZ_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011533963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6248139.1"
                     /translation="MERMSLEFVGKVAVLKFNHPEVMNAVGSQMLLDFADALKDIAHP
                     GNGARCLLLTGEGRGFCAGANLQDGERSERRGGAGDGLRASYHPILLTLRDLNMPMVT
                     AVNGAAAGVGMSFAMMGDIVCASSNAFFLQAFARIGLVPDGGSTFLLPRLVGWGRAME
                     LSMLAERLPAEKAHEWGLVNRLFDDNAALLEGAMAIADRLASGPRSLALIRQAYWNTW
                     HNAFEQQLDLEARLQNQAGASRDFAEGVAAFLEKRDAEFTGE"
     gene            3821..4270
                     /locus_tag="AC16UZ_15495"
     CDS             3821..4270
                     /locus_tag="AC16UZ_15495"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6248140.1"
                     /translation="MDNQRIAEIQRDCEALSLAYARAIDFRDYDHFVSLFTDDAVLDL
                     GRPLRGREEIHAAISARPDELRSRHVITNVFVDVVDENTARGIAYLTLYRHIADKPSI
                     PGPGSEPAPTTLPAAVGHYQDRFARGPDGWRFEARSLHLAFRDPAAF"
     gene            4348..4827
                     /locus_tag="AC16UZ_15500"
     CDS             4348..4827
                     /locus_tag="AC16UZ_15500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6248141.1"
                     /translation="MAELKVCDVKLAAIRDAGLIARMSRDYIEHGLRWRWRAPQIRAK
                     IRHPETAVIVAEVELGGVRFIGGFASMDFGEDKAALNLLAVHPNLRRRGIGGNLVEWL
                     HKSAGVAGCQHIELQVRAGNVSARRFYGRMGYTEEALINGYYDGKEAAYQMKFRLAQ"
     gene            4824..>5365
                     /locus_tag="AC16UZ_15505"
     CDS             4824..>5365
                     /locus_tag="AC16UZ_15505"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6248142.1"
                     /translation="MNPVETAAGFAAEIRRRAPEIEQHRRMPGDLANRMAGAGLFRLL
                     VPELYGGLQVEPLAFFETLKETARADGAVGWCQMIGVTTGMMAASLPDEWSRTIYGCN
                     PDTITSGVTAPLGIAVAEGDGLRVSGRWPFGSGCEVSDWICGGCQLMEDGEPRKNRFG
                     SPEIALVFFPADDVTIHDTW"
BASE COUNT          918 a         1860 c         1673 g          914 t
ORIGIN      
        1 ttctggatca gcgccgtcga tccggcctcg ccctgcgccc tcgtcaccgc catacccgac
       61 ctgccggccc gccgaccgaa caattatctt gcttttcacg agggatccct ggccctggtg
      121 atcgaaaatg ccggcaagcg cctgcaattc caccgcgggc cggacgatgc ctgcctcggt
      181 cgggtctgcg agcccctggc ccatctcgtc cggcgccgca ggcgggtgga ggtagagctc
      241 atcaacgacg ttcccgcccg ctcgagcccc tacctggaac cgttggggcg tttctttcac
      301 gctgtgaccg accacaagca attaatcctg atgatcggct agcttcggca cttgtgcggg
      361 cattttccgc gtgagtttta agaggtcgat cgtgatcgtg tgcgccctga tcaaggacga
      421 gcagctgcga gaccggttgc ggcggctggg agcccgcgcc ggctggcgcg tcctcttcgc
      481 ggctgacgat cgcgaccttc tcgtaaccct gatcagcggc aaggcaacgc cggacgtcat
      541 cgtaagcgac gagccggaca atctgaccgg agtcggcgcg ctgcccacga ccaggattta
      601 cctgcggccc gacagaggcg agcccggcac ggacgcgttc gatcctgctc tggatgacca
      661 ggccttgctc cagcacatca acaactgcgt gaatgcccga cgcttccggg cccgattcgc
      721 cgccatcgat cgcaacgagc cgatcaccag cctgccgcga cacgaggagc tgttcgatgc
      781 gctgtcgagg catgtcgacg agcccgtggg gatgatggtg gtcaagctcg atcatgcgga
      841 gcatctctat gggagcctgg atccggtcgc gaagacggac cttctcgccg cgctcagcca
      901 gaggctggag tcaaagctgc ccactcaggc ccgactcggg ttctacgacg ccacctgctt
      961 cgtggtcacc ctgcccagaa tccagtcgtc agagctcgag cggctgggca acgatcttcg
     1021 cctggcggtg agcgcaccgc tcatcgacgc gggcggggag atccatctga cgatcagcat
     1081 cgggtgcagc ttcgccagct ccttcaacgg cgccgatcat ctgtgggcca gcgcctgggc
     1141 tgccgccaac gctgcctctc aggctggcgg ggaccgggtc ctcggcgcaa accccgacac
     1201 cgcgatctcc gaacgcatcc ctaccgccct ggagcgggac gagttctcgc tgctgctgca
     1261 gccccagtgg agtctgcagg acaaccggct gaccgccgtt gaggcgctgc tgcgctggca
     1321 gggccgcgac atcggcgggc ttgcccccag ccacttcatt cccctggtcg agcagcaggg
     1381 acagatggcc cgggtgggtg actgggtttt agagcgcgcc tgccgcgaga ccgccgcgtg
     1441 gcttccccgc atggtgcagc ccattctgct gacggtaaac gtatcgcccc agcagtttca
     1501 cggccgcagc atcgtcaccc agatcgcccg caacagcgcc gagcaccggc tcaaccccgc
     1561 gtccctggag ctggagctgt cgcaacgcaa catgctgcga ctggtggacg actaccgcga
     1621 gcagatgtat accctgcgcg actggggcgt ccgctttgcc atcgacaatc tcggcggcgg
     1681 gctcatcgag cccgagaaac ttctgcgctg cccggtggac acgctcaaga tcgaccgagc
     1741 cgtggtgagc gcgttgtccg aggataccca ggcggccgat ctcgccgcgc agatctgcga
     1801 gttgggggcg cgattcaacc tgcgcgtcgt cggggttggc gtcgagaccg aggagcagct
     1861 caaattgctc gccgagtggg gctgcacgga cgcccagggg ctgctcgtct ctcgtccggt
     1921 accggtaacc ggaatccagc acctgatgaa tcgcctcgcc gacttaaact gaccccgctg
     1981 cttccttccg gggaccctgc taccatcggc cgatgctgct gatcactcac cgggactgcc
     2041 tgctgcacga gatgcagcac cggcatcccg agcgacccga tcgtcttcgc gcggtgctgc
     2101 aactgctcga gtccagcggt ctgtcccggg acctgaagca tgcagaggcc ttgccggcga
     2161 gccaggaagc gctgcgagcc gtgcacgatc cggattacct gcgcacgctg tccgaatcgg
     2221 tgcccgagtc cggactgctg cccgttgatc cggacacggc catggggcca tccagcctgc
     2281 gggcggccgc cctggccgcc ggcgcggttg ccgacggcgt tcgcgccgtc ctcgagggag
     2341 accaatccac agtgttctgt gccgttcgcc ctccaggtca tcacgccgag cacgacgcgg
     2401 ccatgggttt ctgcttctac aacagcatcg ccctgggggc cgtcaccgcc ctcgaagacc
     2461 cccgcgtaga gcgcgtcgcc gtcctggact tcgacgtcca ccacggtaac ggcacggtgg
     2521 acatattcaa ggaccggccc gaggtgctgg tctgctcgag cttccagcac ccgttctacc
     2581 cccaccgcta cgcagacctg gcgcgcccca acatcgtcaa cacgcccctg cccgcaggca
     2641 ccgcaggcgc cgactttcgc cgggccgtgg aggcgcagtg gcttcccgcc atcgacaacc
     2701 accagcccca gctgatactg gtctcagcgg ggttcgacgc ccaccgcctc gaccccctgg
     2761 ctcagctcga gctgtgcgag gacgatttcc gctggatcac ccggctcatc gtcgccagcg
     2821 cgaagaccca tgccaacgga cgcatcgtct ccgctttaga aggtggttac gacctcgccg
     2881 cgctggccgc atcggctcat gcgcacatcg aggagctgct cgccgcgtag tcgttactcg
     2941 ccggtgaact ccgcgtcgcg cttctctaag aacgcggcca cgccctcggc gaagtctctc
     3001 gacgcgccgg cctgattctg cagtctggct tcgagatcga gctgctgctc aaacgcgttg
     3061 tgccacgtgt tccagtaagc ctggcgaatc agggcaagcg atctcggccc gctggccaac
     3121 cggtcggcga tggccatggc accttcgagc agggccgcgt tgtcgtcgaa caaacggttc
     3181 accagccccc actcgtgggc tttctccgcc ggcagccgct cggccagcat ggagagctcc
     3241 atggcgcggc cccatcccac caggcggggc agcaggaagg tcgagccacc gtcgggcacc
     3301 agaccgattc gcgcgaacgc ctgcagaaag aaggcgttcg aagatgcgca gacgatatcg
     3361 cccatcatgg caaagctcat gccgacgccg gcagcggccc cgttgacggc ggtgaccatc
     3421 ggcatgttga gatcccgcaa ggttagaagt atcggatgat agctggcacg caggccgtca
     3481 ccggcgccgc cgcggcgctc ggagcgctcg ccatcctgaa ggttggcgcc ggcgcagaag
     3541 ccgcgcccct cgccggtgag cagcaggcag cgggcgccat tgcccggatg agcgatgtcc
     3601 ttcaacgcgt cggcgaaatc cagcagcatc tggctgccga ccgcattcat tacctcggga
     3661 tgattgaatt tgagcacggc caccttgccc acgaactcca gcgacattcg ttccattggt
     3721 tcccccaatc tgaaagcgcg accgacttgt cgatctgcga cggatcggga acgataacca
     3781 acccgatggc caggctcaaa ccaggaggga gaaacccgac gtggacaatc agcggatcgc
     3841 ggaaatccag cgcgactgcg aagcgctctc cctggcatac gcccgcgcca tcgacttccg
     3901 ggactacgac catttcgtct cccttttcac cgacgacgcc gtgctcgacc tcggccgccc
     3961 gctgcgcggg cgcgaggaga ttcacgccgc catctcggcg cggccagacg agctgcgctc
     4021 gcgtcacgtc ataaccaacg tgttcgtaga cgtcgtcgat gaaaacactg cccgcggcat
     4081 cgcttatctg accctctatc gccacattgc cgacaaaccg tcgataccgg gtcccggaag
     4141 cgaacccgcg ccgaccacgc ttcccgccgc cgtcggccac taccaggacc ggttcgcccg
     4201 cgggccggac ggatggcggt tcgaggcgcg cagcctgcat ctggcgttcc gcgacccggc
     4261 tgccttctga ccggttctac aagtcgtttg caacctcggc gcccggaccc gttcccctcg
     4321 ggatggggga ttggcaataa tcggtcaatg gccgaattga aagtctgcga tgtcaagctg
     4381 gccgcaatca gagacgcggg gctgatcgcg cgcatgtccc gggactacat cgagcacggg
     4441 ctccgctggc gctggcgcgc gccgcagatc cgcgccaaga tccggcatcc cgaaacggcc
     4501 gtcatcgtcg cggaggtgga gctcggcggc gttcgcttca tcggcggttt tgcctcgatg
     4561 gatttcggcg aggacaaggc cgcattgaac ctgcttgccg tgcacccgaa tttgagacgg
     4621 cgcggcatcg gtggaaacct cgtcgagtgg ctgcacaaga gcgcgggcgt ggccggctgc
     4681 cagcacatcg agctgcaggt cagagcgggc aacgtgtctg cgcgtcgttt ctatggtcga
     4741 atgggctaca ccgaggaagc cctcatcaac ggctactacg acggcaagga ggccgcttac
     4801 cagatgaagt tccgcctggc ccaatgaacc cggtcgagac cgccgcaggc ttcgctgcgg
     4861 aaatccgccg ccgtgctccg gagatcgagc agcatcgccg gatgcccggc gacctcgcaa
     4921 atcgcatggc tggtgcggga ctgttccgcc tgctcgtccc ggagctctac ggcggcctgc
     4981 aggtcgagcc tctagctttc ttcgaaaccc tcaaggagac ggcccgcgcc gacggggcgg
     5041 tcggctggtg ccagatgatc ggcgtgacca ccgggatgat ggccgcctcg ctgcccgacg
     5101 aatggtctcg cacgatctac ggttgcaacc ccgataccat cacttccggc gtcaccgccc
     5161 cgctgggcat cgccgttgcc gaaggcgacg gtctcagggt cagcggccgt tggcccttcg
     5221 gcagcgggtg cgaggtctcg gattggatct gcggcggctg ccagctcatg gaagatggcg
     5281 agccgcgcaa gaaccggttc ggctcgcccg agatcgccct ggttttcttt cccgcagacg
     5341 acgtgacgat tcacgatacc tggca
//
LOCUS       DBNHOX010000713         3191 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_122221, whole genome shotgun sequence.
ACCESSION   DBNHOX010000713 DBNHOX010000000
VERSION     DBNHOX010000713.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_122221"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="AC16UZ_15510"
     CDS             complement(<1..729)
                     /locus_tag="AC16UZ_15510"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6248143.1"
                     /translation="MILTSNQIDDYWAQGFVVVPDLIREVSLDRYEARFEELAIGSVA
                     PAPAMKIMRDIMFVRGAALPESALHAVNKMINFEDDAVLYGYTLEPRLVSAVRDLIGP
                     DIYSISTNIFNKPPGVDGRHPLHQDLRYFRIRPADGIVGTWTSFAATTRDSGCLAAVP
                     GSHKGDLLPHADPDWEYVNAGFLGIENQTEAQRVYLEMDPGDTLLFHPLLVHGSGRNR
                     SDHFRRAISSHYASAACESPVRDWR"
     gene            complement(791..1633)
                     /gene="purU"
                     /locus_tag="AC16UZ_15515"
     CDS             complement(791..1633)
                     /gene="purU"
                     /locus_tag="AC16UZ_15515"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003118346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HIR6248144.1"
                     /translation="MADYVLTFSCPDRPGLVHAVSSWLVDLNGNILDSDQFRDDTTER
                     FFMRVHFEAPDDLPALNQTFIPTAGAMNMDWQIWPTARRPRVLMLVSKIDHCLADLLY
                     RRRIGELKIDMPLIASNHRDAYPLAAAHDVDFLHLPVSPETKSQQEARLLDEVRARDI
                     DFVVLARYMQILSAEFCAALTGRIINIHHSFLPGFKGARPYHQAHERGVKLIGATAHY
                     VTADLDDGPIIEQDVERVDHRMSADQLAAVGRDIERVVLAKAVRYQSEHRILLNGNRT
                     VVFT"
     gene            complement(1927..>3191)
                     /locus_tag="AC16UZ_15520"
     CDS             complement(1927..>3191)
                     /locus_tag="AC16UZ_15520"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6248145.1"
                     /translation="VGLILMLARISDVITDPLIGYLSDRTGGRFGRRKPWLAAGALLM
                     MVSAFQLFNPNALSAAPVDNWHLLSWSVLLWLGWTMINIPYYAWGAELSDDYNERTRI
                     TGWRQAFGFLGNVSVLAVPVIAGEITGYGSMPREGLTIIGSMALVALPTLIAVTLWRV
                     PEREAYAKPKASVLTHARAMSRNGSFILLFLGFMLMSLGTGWGSATFMLFATYVVGAE
                     GQTQAILLGYFGANVIALPLWVALAQRIGKKTTWLIGGVLFVIVTPCFLLLEHGDLWW
                     FFLCLALYGVAGGNFAALSMSMKADVIEIAARRSGENMAGSYIAVWSLGQKMVAALAL
                     GLALPFLQLLGFDPAGGNDAEQIRALALVYVVPPWLFYALAVVVIWRYPITASRLQRI
                     RAAFERRDARLERKRIGRAGFGEQVEPT"
BASE COUNT          612 a          975 c         1048 g          556 t
ORIGIN      
        1 gcgccagtct ctgacgggcg attcgcaggc cgcggaagcg tagtgtgaag agatggcccg
       61 tcggaagtgg tccgaccggt tccggccgga accgtgcacc agcagcggat ggaacagcag
      121 ggtgtcgccc ggatccatct ccaggtatac gcgctgggct tcggtctggt tttcgatgcc
      181 caggaatccc gcgttgacgt attcccagtc agggtccgca tgcgggagca ggtccccctt
      241 gtggcttccg ggcaccgcgg cgaggcatcc ggaatcccgg gtcgtcgcgg caaacgacgt
      301 ccatgtgccc acgatgccgt ccgccggcct tatccggaag tagcgcagat cctgatgcag
      361 cgggtggcgg ccgtccacgc cggggggctt gttgaagatg ttcgtcgata tgctgtaaat
      421 gtcgggcccg atcagatcgc gcaccgcact caccagccgg ggctcgaggg tgtatccgta
      481 gaggacggca tcgtcctcga agttgatcat cttgttcacc gcgtggagag ccgattcggg
      541 cagcgccgct ccacggacga acatgatgtc gcgcatgatc ttcatggccg gcgcgggcgc
      601 gaccgagccg attgccagtt cctcgaagcg cgcctcgtaa cgatccagcg agacctcgcg
      661 gatgagatcg ggcaccacca cgaagccctg ggcccagtag tcgtcgatct gattgctcgt
      721 cagtatcatt agcccgtttc gactgcaggc cgcgcttggc acccatcggg gggctggcgc
      781 ccgccaagcc tcaggtgaaa accaccgttc tgttgccgtt cagcaggatc cggtgttccg
      841 actggtatct taccgccttt gccagcacga cgcgctcgat gtcgcggccc accgctgcga
      901 gctggtcggc cgacatgcgg tggtcgacgc gctccacgtc ctgttcgatg atgggcccgt
      961 cgtccaggtc cgctgtgacg taatgcgcgg tagcgccgat cagcttgacg ccccgctcgt
     1021 gagcctggtg ataaggacgg gcgcccttga agccgggcag aaacgagtga tggatgttga
     1081 tgatccggcc ggtcagggcg gcgcagaact cggcgctgag tatctgcatg tagcgggcca
     1141 ggacgacgaa gtcgatgtcc cgggctcgca cttcgtccag gagacgcgcc tcctgctgcg
     1201 atttggtctc ggggctgacc ggaaggtgca gaaagtcgac gtcatgggcg gccgccagcg
     1261 ggtaggcgtc gcgatgattc gaggcgatca agggcatatc gatcttcagc tccccgattc
     1321 gcctgcggta caggagatcg gccaggcagt ggtctatctt ggagaccagc atgagcactc
     1381 ggggccgtcg cgcggttggc cagatctgcc aatccatgtt catggcaccg gcagtcggga
     1441 tgaacgtctg attcaaggcg ggaaggtcgt cgggcgcctc gaaatgcacc cgcatgaaaa
     1501 agcgttccgt ggtgtcgtca cggaactgat cactgtcgag tatgttcccg ttcaggtcca
     1561 ccagccagct cgataccgca tgaaccagcc cgggacgatc gggacatgag aaagtgagaa
     1621 cgtaatcagc caagcacgaa cacgccgcaa ttccagcggc gtgcatcgta cctgcgatca
     1681 gcctccaaag cgaacgcgaa tggtccttcc ggcaatggca agcgccatgc cgcccgccct
     1741 tacggcgatg ttcagacctt gatcagcccc gctgcgatgg cgatgagcgt gatcgtgatc
     1801 ccggtaacgg ccacgaggcc aagtgccatg ctgatgtgtt gcgcggtttc gaccatgatt
     1861 gatctccagc cattgattga tcatgacctt tttaaggctg atgacggctt tggagaatca
     1921 ggggatctac gtaggctcga cctgctcgcc gaaaccagct cggccgatgc gcttacgctc
     1981 caggcgcgcg tcgcggcgtt cgaaagccgc ccggattcgt tgcaggcggg aagcggtgat
     2041 cgggtagcgc catatcacca ccaccgccaa cgcgtaaaac agccagggag gaaccacgta
     2101 gacgagcgcg agggccctga tctgctcggc atcgttgccg ccggcagggt cgaaaccgag
     2161 cagttgcaga aagggtagcg ccagacccag agccagggcg gcgaccatct tctggccgag
     2221 ggaccagacc gcgatgtagg aacccgccat gttctcgccg gaccggcgcg cggcgatctc
     2281 gatcacgtct gccttcatgg acatcgacag cgcggcgaaa ttgcccccgg ccaccccgta
     2341 gagtgccagg caaaggaaga accaccagag atcgccgtgc tccagcagca ggaagcaggg
     2401 ggtgacgatg acgaagagca cccctccgat cagccaggtg gttttcttgc cgattcgctg
     2461 ggcgagggcc acccacagag gcagggcgat aacgttcgcg ccgaagtaac cgagcagaat
     2521 ggcctgagtc tgtccctcgg ccccgacgac gtaggtggcg aacaacatga acgtcgccga
     2581 tccccagccg gtaccgaggg acatgagcat gaatcccagg aacagaagga tgaaggagcc
     2641 gttgcgtgac atcgcccgcg cgtgggtgag caccgatgcc ttcggcttcg cgtaggcctc
     2701 gcgttcgggg acgcgccaga gggtcacggc gatcaacgtg ggcagcgcca cgagcgccat
     2761 ggatccgatg atcgtcaggc cctctctggg catgctgccg tatccagtga tctcgccggc
     2821 aatgacgggc actgccagga cgctcacgtt cccgaggaag ccgaacgcct gccgccatcc
     2881 ggtgatgcgg gtgcgttcgt tgtagtcgtc ggagagctcg gcgccccagg cgtagtaggg
     2941 gatgttgatc atggtccagc caagccagag cagcacgctc cagctcaaca ggtgccagtt
     3001 gtccaccggt gctgcgctga gcgcgttcgg gttgaagagc tggaatgccg agaccatcat
     3061 caggagcgcg cccgccgcga gccagggctt gcggcggccg aagcggcctc ctgtgcggtc
     3121 gctgagatag ccgatcaacg ggtcggtgat cacatcggag atgcgcgcca gcatcagaat
     3181 gaggcccacg t
//
LOCUS       DBNHOX010000714         1927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1022976, whole genome shotgun sequence.
ACCESSION   DBNHOX010000714 DBNHOX010000000
VERSION     DBNHOX010000714.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1022976"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="AC16UZ_15525"
     CDS             complement(<1..437)
                     /locus_tag="AC16UZ_15525"
                     /inference="COORDINATES: protein motif:HMM:NF016300.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR6248146.1"
                     /translation="MSRIQIIALLCCAMLLAAPTIGRPQVEGIHVSGRGEVLVTPDMA
                     RVTLRVTRDGQDAVELKKELDDVTRKVLSLTRSLKIEGEDVTAAVVNIQPRYRRGGGQ
                     SVIDGVTASRTIAVVLRDLDRYGQLMNGALEVGVNSVSGTQLDS"
     gene            483..>1927
                     /locus_tag="AC16UZ_15530"
     CDS             483..>1927
                     /locus_tag="AC16UZ_15530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like protein"
                     /protein_id="HIR6248147.1"
                     /translation="MSKKELRLAVVIYGGASLAVYMHGVTKEMLKLVRASKVFHQMGI
                     GRARYASYAEGPDNRQADTEAVYFEILKEINGRSHFRVVLDVIAGASAGAINSVMLAK
                     AIVDDASLDSQTGTWLSDADVEYMGRETSPWQKWWLYPILRLLFFWLPRDVGASPETR
                     EKLARVVRSSWFQPPLSGARLSHIFFNSLEAMLKTRLPGSSLLPAGQRLDVYTSITDL
                     VGYPRSIRLHDELMASEREHGAYCRFTHMETEAGRRLSDLDDANLPGLVWAARASSSY
                     AGAFEPFHHSELKRVLAERHLSWPLEEKFLHYNVFVRDGTPAARLFDPADRYFVDGGI
                     VNNKPFEVALEALGHRPADRLVERCIVYIEPDPSAVDVVSPERNLGYLRTIRAALSSI
                     PRNQPIVDDLQAIVSQDKRVRINRRIVESNAAEINTLVRELQDVARRQEMTADLITYL
                     RTAILERAEEDMSIAYHAYVQRRVWRLTDALV"
BASE COUNT          331 a          629 c          611 g          356 t
ORIGIN      
        1 gagtcgagct gggtaccgct taccgagttc acccccactt ccagagcgcc gttcatgagc
       61 tggccgtaac ggtcgagatc gcgcaatacc accgcgatgg tgcgggatgc ggtgaccccg
      121 tcgatgacgc tctggccgcc cccacggcgg tagcgaggtt ggatgttgac caccgccgcg
      181 gtcacgtcct caccctcgat cttcagagag cgcgtcaggg acagcacctt gcgtgtcacg
      241 tcgtccagct cttttttgag ctcgacggcg tcctgcccgt cgcgggtgac gcgaagggtg
      301 acccgggcca tgtccggggt cacgagcacc tcgccccgac cgctcacatg aattccttcc
      361 acctggggac ggccgatggt gggggcggcg agcagcattg cgcagcacag cagggcgatg
      421 atctggattc gggacatggg tcgtactccg ttgggcagtt cgcgccacta taatggctaa
      481 ccatgtcgaa gaaagaacta cggctcgcgg tggtgatata tggcggggct tccctcgccg
      541 tctacatgca tggggtcacc aaggagatgc tcaagctcgt ccgcgcctcc aaggtttttc
      601 accagatggg catcggccgt gcccgatacg cttcgtatgc cgagggtccc gacaaccgcc
      661 aggcagatac cgaggccgtc tatttcgaga ttctcaagga gataaatggg cgcagtcact
      721 tccgggtggt tctggacgtc atcgccggcg cgtcggcggg cgccatcaac tcggtgatgc
      781 tggccaaggc catcgtcgat gatgcgtcgc ttgattccca gacgggaacc tggctgtccg
      841 atgcggacgt ggagtacatg ggcagagaga cgtcgccctg gcagaagtgg tggctttacc
      901 cgattctccg gctcctgttc ttctggcttc cgagggacgt cggtgccagc ccggaaaccc
      961 gcgagaagct cgcccgcgtc gtgcgctcct cctggttcca gccgccgttg tcgggggccc
     1021 ggttgagcca catcttcttc aactctctgg aggccatgct caagacccgg ctcccaggga
     1081 gcagcctgct gcccgccggt cagcgtttgg acgtgtacac gagcatcacg gacctggtcg
     1141 gctatccccg cagcatccgc cttcacgacg agctgatggc cagtgagcgg gagcacggtg
     1201 cctactgccg attcacccac atggaaaccg aggccggccg ccggctcagc gacttggacg
     1261 acgccaacct gccgggccta gtctgggcgg cccgggccag ctcgtcctat gcgggggctt
     1321 tcgagccgtt tcatcactcc gagctcaagc gcgtgctcgc cgagcggcac ttgagctggc
     1381 cgctggagga gaaattcctg cactacaacg tgttcgtccg ggacggcacg ccggcggcgc
     1441 gtcttttcga cccggcggac cgatacttcg tggacggcgg catcgtcaac aacaagcctt
     1501 tcgaggtggc cctggaagcc ctcggtcacc gtccggccga tcggctcgtc gaacgctgca
     1561 tcgtctacat cgagcccgat ccgagcgccg tggacgtcgt gagcccggag cgtaacctgg
     1621 gatacctccg cacgatccgc gcggcgctct cctcgattcc ccgcaaccag cccatcgtag
     1681 acgatctcca ggccatcgtg tcccaggaca agcgggtgcg catcaaccgg cgcatcgtcg
     1741 aatccaatgc ggccgagatc aataccctgg tccgcgagct gcaggacgtg gccaggcgcc
     1801 aggagatgac ggcagatctg atcacctacc tgcggacggc tattttggag cgcgccgagg
     1861 aggacatgag cattgcctat cacgcctacg tgcagcggcg cgtctggcgg ctgaccgatg
     1921 cgctcgt
//
LOCUS       DBNHOX010000715         1975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_893128, whole genome shotgun sequence.
ACCESSION   DBNHOX010000715 DBNHOX010000000
VERSION     DBNHOX010000715.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_893128"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="AC16UZ_15535"
     CDS             complement(<1..1195)
                     /locus_tag="AC16UZ_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HIR6248148.1"
                     /translation="MSTQIEQQQSRLKELIAKGKEQGYITYSEVNDHLPDDISDPDQI
                     EDIIRMINDMGITVYETAPDADELLSTEENTADELAAEEAAAALAAVETEAGRTTDPV
                     RMYMREMGTVELLTREGEIAIAKRIEEGIRDMLSAMAYFPGTVEYLLEVYSEANAEGR
                     LSDMLVGYLDPTDNVPQAAQVQPGAQNQKDDDDEEEDKGPDPVEAKRRFTALKRQYNK
                     TYKVIAEKGRNSKEAQAQLEKLGVAFSPFKLVPRHFDELVDMPRMALAIVRRHERIIM
                     NACVRQARMPRAVFRKEYVGSETSVAWVNKHIKAGKDYSKGLDAQKDEIARAQKKLKA
                     LVKETGLTVGEIKDINRRVSLGEAKARRAKKDMVEANLRLVISIAKKYTNRGLQFLDL
                     IQEGNI"
     gene            complement(1396..>1975)
                     /locus_tag="AC16UZ_15540"
     CDS             complement(1396..>1975)
                     /locus_tag="AC16UZ_15540"
                     /inference="COORDINATES: protein motif:HMM:NF019883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248149.1"
                     /translation="RFASLAMPHIDRLPAGILKELMASRLEGLTGFRPSAQARPSRGP
                     ARRTAADVSLTPLARRLVGYLLREPSVVSALSEEARRALRPLAGNDLLAELVTYLDEH
                     PDAETPELLGRWTGHDLHDELTGLARQPSAIDIAAVRGDFTEGVARYLEMQQASARRQ
                     LISEMHQDPSAERLKDYWSLKQGPRDKVSGTD"
BASE COUNT          331 a          648 c          586 g          410 t
ORIGIN      
        1 caatgttgcc ttcctgaatc aggtccagga actgcaggcc gcggttggtg tacttcttgg
       61 cgatggagat caccagacgc aggttcgcct ccaccatgtc cttcttggcg cggcgggcct
      121 ttgcctcacc cagcgacacc cgccggttga tgtccttgat ctcgccaacg gtgaggcccg
      181 tttccttcac cagcgctttc agcttcttct gagcccgggc gatctcgtcc ttctgcgcgt
      241 ccagaccctt cgagtaatcc ttgccggcct tgatgtgctt gttcacccag gcaacgctgg
      301 tctccgagcc cacgtactcc ttccggaaga cggcgcgagg catccgcgcc tggcgcacac
      361 aggcgttcat gatgatgcgc tcatgacgcc ggacgatggc tagcgccatc cgcggcatgt
      421 ccaccagctc atcgaagtgg cgcggaacca gcttgaaggg cgaaaacgcc acgcccagct
      481 tctccagctg agcctgggct tccttgctgt tgcggccttt ctccgcaatg accttgtacg
      541 tcttgttgta ctggcgcttc aacgcggtaa agcggcgctt tgcctccacc ggatccggac
      601 ccttgtcctc ttcctcgtcg tcatcgtcct tctggttctg agcgcccggc tgcacctgag
      661 ccgcctgagg aacgttgtcg gtggggtcga gatagccaac gagcatgtcg ctcaagcgcc
      721 cttccgcatt ggcctcggag taaacctcca ggaggtactc cacggtgccg gggaaatagg
      781 ccatggcgga gagcatgtcg cggatgccct cctcgatgcg cttggcaatg gcgatctcgc
      841 cttcgcgggt aagaagctcc accgtgccca tctcgcgcat gtacatgcgc accgggtcgg
      901 tggtacggcc cgcttccgtc tccaccgcgg cgagcgcggc ggccgcttcc tcggcagcga
      961 gctcgtctgc cgtattctcc tccgtggaga gaagctcgtc ggcatcgggc gcggtctcgt
     1021 acaccgtgat gcccatgtcg ttgatcatgc ggatgatgtc ttcgatctga tcaggatcgg
     1081 agatatcgtc aggaagatgg tcgttcacct ctgaataggt gatatagccc tgctccttgc
     1141 ccttggcaat gagctccttc agccgggatt gctgctgttc aatctgcgtg ctcatgacac
     1201 ctcaaatagt gaaatgcgtt ggggcagaga ggttccccgc attacagcat ccctgactta
     1261 atgctgtggt cgtgggttcc cgcccggatt cgcggactgc cgaaaatgag ttaacagccg
     1321 aatataccat atataggggc gcttactgaa agttaaacaa ttgtccgaca aaaaagtcgc
     1381 ttgcgcaccg cctggctaat cggtgccgct gaccttgtct cgagggccct gctttaaaga
     1441 ccaatagtcc ttcaggcgct ccgccgacgg atcctggtgc atctcggaga tcagctgccg
     1501 cctggcagac gcctgctgca tctccagata tcgggccacg ccctcggtga aatcgccacg
     1561 caccgccgcg atgtcgatgg ccgacggctg ccgggcaaga cccgtcagct cgtcgtgaag
     1621 atcgtgcccc gtccagcgcc cgagcagctc cggcgtctcg gcatccgggt gctcgtccag
     1681 ataggtcacg agttcggcca gtagatcgtt ccccgccagc ggccgcaggg cccggcgggc
     1741 ctcttccgaa agggctgaga ccacggacgg ttcgcgcagc agatacccga ccaggcgtct
     1801 cgccaggggc gtcaggctga catcggcagc cgtccggcgc gccggccccc tcgatggtcg
     1861 cgcctgggcg ctcggccgga agcccgtcag cccctccagc cgggaggcca tgagctcctt
     1921 taggatgccc gcgggcaacc ggtcgatgtg cggcatggcc aggctcgcga acctg
//
LOCUS       DBNHOX010000716         2351 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_251570, whole genome shotgun sequence.
ACCESSION   DBNHOX010000716 DBNHOX010000000
VERSION     DBNHOX010000716.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_251570"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="AC16UZ_15545"
     CDS             complement(<1..219)
                     /locus_tag="AC16UZ_15545"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR6248150.1"
                     /translation="MPVLPVLPLKDTVVYPQIVRPLGVGRRRSLAALNHAVEGPREII
                     TVAQHDGANEEPGLADLYAVGCIASISRV"
     gene            complement(230..1297)
                     /locus_tag="AC16UZ_15550"
     CDS             complement(230..1297)
                     /locus_tag="AC16UZ_15550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6248151.1"
                     /translation="MEFGLSEEQTLLQDSLNGFLDANCPLDRVRRFAEEPVAADLHSG
                     LAELGVFGLLVPEEAGGVGLGCFEAALVAEALGSHTAPCAFLGSAVMAPLALTLAGNE
                     PTDTLSRLATGELTVGVAVAEFVGARADGGITADGETLSGKALSVLDFDAQAYLVADA
                     AGSTYLVDAGAPGVFAEALPTIDKTRRTGALTLNGAPAQLICDDPDVLARVIDAGRVM
                     LAADTLGAAQNMLDQAVEYAMQREQFNRPIGSFQAVKHMCAEMAASLEPCRAMVWYAS
                     HALDDLPAEASLCACHAKAHLSEVGKFVAKTATEVHGGMGFTDLLGLHYWFKRIGYNR
                     QMLGAPELLREQAARLQGLCA"
     gene            complement(1307..>2351)
                     /locus_tag="AC16UZ_15555"
     CDS             complement(1307..>2351)
                     /locus_tag="AC16UZ_15555"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6248152.1"
                     /translation="YAARTIPREYGGYGAAPDILESRIIAEEFAAARVSSGLGGQGIS
                     MLVPTLLEMGTQEQKTTFIPPTLRGEMLWCQGYSEPGAGSDLASLSTRAELDGDEWVI
                     NGQKIWTSTAHIADWMFCLVRTEPDAPKHQGISFLLLRMDTPGIEVRPLVDMTGAANF
                     NETFFTDVRVPKDQIVGRRGEGWLVANAILGHERDSLGDPNVMLSRFNALTELMKTET
                     LGGERLIDHPVFRDRLMQIQGRVMALSYNDLRLLSAKVNAADARLARMIVKLQGTELR
                     HDLEGLAVDAMGELGLAYGDNPYLRDHGSWQSQYMFYLGLIIGGGTSQIQKNIISERG
                     LGMPKEPKYEKQA"
BASE COUNT          372 a          775 c          795 g          409 t
ORIGIN      
        1 cacgcgactg atgctggcga tgcagccgac ggcgtagaga tccgccaggc ccggctcctc
       61 gttggcgcca tcgtgctggg caacggtgat gatctccctg ggcccctcca ccgcgtggtt
      121 cagggccgcc agagagcgac gccgacccac gccgaggggg cggacgatct gcgggtagac
      181 gaccgtgtct ttaaggggca ggacggggag gactggcatg gcgttctcgt caggcgcaca
      241 ggccctgaag gcgggctgcc tgctcgcgca acaactccgg agctccgagc atctggcgat
      301 tgtagccgat ccgcttgaac cagtagtgga gtccgagaag gtccgtgaag cccatgccgc
      361 cgtgtacctc ggtcgccgtc ttggcgacga acttgccgac ctccgaaagg tgggccttgg
      421 cgtggcaggc gcacagggag gcttcggcgg gaaggtcgtc gagcgcgtgg gatgcgtacc
      481 agaccatggc tcggcacggt tcgaggctcg cggccatctc tgcgcacatg tgcttgacgg
      541 cctgaaacga gccgatgggc cggttgaact gctctcgctg catcgcgtat tccaccgctt
      601 gatcgagcat gttctgggca gcgccgaggg tgtccgctgc gagcatgacc cgtcccgcat
      661 cgatcacccg ggcgagcacg tccgggtcgt cgcagatcag ctgtgccggc gccccgttca
      721 gcgtgagcgc gccggtgcgg cgggtcttgt cgatggttgg aagcgcttcg gcgaacaccc
      781 cgggcgctcc ggcgtcgacg aggtaggtgc tccctgccgc gtccgcgacc aggtaagcct
      841 gggcatcgaa atccagaacg gagagcgctt tgccggagag cgtctccccg tcggcggtga
      901 tgccgccatc cgcccgcgcg ccgacgaact cggccacggc gaccccgacc gtgagttctc
      961 ctgtcgccag tcgggacagg gtatccgtcg gctcgttgcc cgccagcgtg agggccagcg
     1021 gcgccatgac ggcggaacct agaaaagcgc aaggggcggt gtggctgcca agggcctcgg
     1081 cgaccagcgc cgcctcgaag cagcccaggc ccaccccgcc cgcttcctcc gggacgagca
     1141 gcccgaagac gccgagctcc gccaggccgg aatgaaggtc ggccgccaca ggctcctcgg
     1201 caaagcggcg taccctatcc agagggcagt tggcgtccag gaagccgttc aagctgtcct
     1261 gcaacagtgt ctgctcttcg gaaagcccga actccatggc ggcctcctaa gcctgctttt
     1321 cgtatttggg ttctttcggc atgccgagcc cgcgttcgct gatgatgttc ttctggatct
     1381 gcgaggtgcc cccgccgatg atcagcccca ggtagaacat gtactggctc tgccagctgc
     1441 cgtgatcgcg caggtagggg ttgtcgccgt aggccagccc cagctctccc atggcgtcca
     1501 cggccagacc ctcgaggtcg tggcgcagct cggtgccctg cagcttgacg atcattcgtg
     1561 cgaggcgggc atccgctgcg ttgaccttgg cggacagcag ccgaaggtcg ttgtagctca
     1621 gcgccatgac ccgaccctgg atctgcatga ggcggtcccg gaacaccgga tggtcgatca
     1681 gccgctcccc gccgagggtc tccgtcttca tgagctctgt aagggcgtta aatcgggaga
     1741 gcatgacgtt cgggtcgccc agagagtccc gctcgtggcc gaggatggcg ttggcgacca
     1801 gccagccctc accgcggcgg ccgacgatct gatcttttgg gacccggacg tcggtgaaga
     1861 acgtctcgtt gaagttcgcc gcgccggtca tgtccaccag cgggcggacc tctatgccgg
     1921 gggtgtccat gcgcagcagg agaaagctga tcccctggtg cttgggcgca tcgggctcgg
     1981 tccggaccag gcagaacatc cagtccgcga tgtgcgccgt gctggtccag atcttctgcc
     2041 cgttgatcac ccactcgtca ccgtcgagct cggcgcgcgt ggacaggctg gcgaggtcac
     2101 tgccggcccc gggttccgaa tatccctgac accacagcat ctcgccgcgc agcgtgggcg
     2161 ggatgaaggt ggttttctgc tcctgcgtgc ccatctccag cagggtcggc accagcatgg
     2221 atatgccctg accgccaagc ccggagctca cgcgcgcggc ggcgaactcc tcggcgatga
     2281 tccgtgattc gagaatgtcg ggcgcggcgc cgtagccccc gtactcgcgt ggaatcgttc
     2341 ttgccgcgta g
//
LOCUS       DBNHOX010000717         4872 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1278992, whole genome shotgun sequence.
ACCESSION   DBNHOX010000717 DBNHOX010000000
VERSION     DBNHOX010000717.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1278992"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="AC16UZ_15560"
     CDS             <1..964
                     /locus_tag="AC16UZ_15560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6248153.1"
                     /translation="LSVGFVLATFMDGRLWMLYLSYGAMALLGAGTIPVTWTRAITTN
                     FFRQRGLALGLTLSGTGLCAVIVPQYAVWMVEAYGWRAAYLGLAAMPLLIAGPVVYFG
                     FRPREGIGGEALETGEAQWGMTLGEAFRTTKYWVLLISIFVVYMAVSGIGPNLIPALV
                     DDGLTPAQAATALSTFGGAIIAGRIIVGYLVDRFWAPGVAALSLSLPVAGCLMLVGAP
                     GFETAVAAALLIGIAAGAELDLMSFLAAKYFGLRHYAKIYAVLYATLAVCSGTAPMLF
                     ARVYDVTASYDVSFWISTVLFALGAVLVLAMGAYPRMGDSRELP"
     gene            complement(1018..2136)
                     /locus_tag="AC16UZ_15565"
     CDS             complement(1018..2136)
                     /locus_tag="AC16UZ_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248154.1"
                     /translation="MQIRTQAAAVLIVLLAGGCAVQQPVQPTGIANITRDQIGRMAVR
                     SPDRPKVALAANVDTKGAAARKTALSAGATWLGGSFEAAGQAGEGGLLIAAFGLVTAP
                     IVAAGGAVYGAAAADSKEEITSGNEVLVRTLDFAPAQMQRALENELTTSAPMDWEFVD
                     PDASNAELAARGFDSVLDMDMDSIASHVSANDFHVYFDTLNRLTLTSLVSGQSLAIRK
                     YWRELPPRAVSDWAGDGGSVLTNALHEQFADIAREMADDLFLYPAIRVHGVEPVSRRR
                     FGAGTISGRRPMFVWSALDGGNGTPAQAVAYELAIAADRQANPVSFRTHKMRYVPPDS
                     LEACRTYSWKVRAHYLSFGEPTTSQWSPEYRFKTPCRK"
     gene            complement(2316..4142)
                     /locus_tag="AC16UZ_15570"
     CDS             complement(2316..4142)
                     /locus_tag="AC16UZ_15570"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIR6248155.1"
                     /translation="MADIFVSYASEDLDRVKPLITALEERRFAVWWDREIHPGTSWDE
                     AIERELESATWIVAIWTANSIRSRWVRTESMAALERDALIPILLDDVTLPVAFRTTQV
                     VDLRQWRPEGSDATFERLVSHLQGKPHPPAEVEAHRQRSPPNRRGPLLAALALTALAA
                     IALVWMVVTEPVRAPSDAGLAVTTFAGQPRIAVLPFDHGSDPEDRPFANGLTDEVIKG
                     LQQYRTIPVISRHASFALRDKGLSVQEIASQLGARYLVTGAVSRRGDRLRVSAGLTDA
                     DGHQLWKESYEVDFDPESVFALQDQIAEQIAGVSYPQILEGEIDRVARRDVTSLDAWG
                     HAVNAVEIINAMNVDRLDEGLLHAERALELDPNLPLAYWARAEIKVYQLVERGLTGQS
                     ADAMEDEILEDFQEALDISPFDGALCGCLGFVRVMRGEIEQAEAVFDTVLPVNPSNAI
                     LRTNYATLLTHQGKLEEARREAELAIRLDPLSRFGSMSWSTLSLVAAMDNDLEQAIQL
                     ARRALQVEGTETYGQVQLPMLLYLKDRIPEAQDAFADLLKHHPRVNPRNRLTYGWFRP
                     IEPLIREHVAQASGTLRKERSLADLLEWIFLDLGWEPPTPMP"
     gene            4374..>4872
                     /locus_tag="AC16UZ_15575"
     CDS             4374..>4872
                     /locus_tag="AC16UZ_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248156.1"
                     /translation="MKGKVLFFSLVFYAVVGMWLWGEYQQRPRVLDGLGQVMLGMTPA
                     QVVGVLGRPTEEFAPAEDEQRRLEYSYRSDGTSDYLVVFWPDEKGEPKADIICREGGS
                     VFGLAVGQSSELDIIDKLGEPSHRSFGPDGRAKAISYESFKVAFEIEGGWTREICVSQ
                     TGSISY"
BASE COUNT          754 a         1527 c         1629 g          962 t
ORIGIN      
        1 gctgtcggtc ggcttcgttc tggcgacgtt catggacggg cgcctgtgga tgctctatct
       61 gtcttatggc gccatggccc tgctcggcgc ggggacgatt ccggtcacct ggacccgtgc
      121 cattaccacc aacttctttc gacaacgggg ccttgccctg gggcttacgc tcagcggcac
      181 ggggctgtgc gcggtgatcg ttcctcagta tgccgtctgg atggtggaag cttacggctg
      241 gcgcgccgcg tacctcgggc tcgcggccat gccgctgctg atcgccgggc ccgtggtgta
      301 cttcggcttt cgcccccggg aggggattgg cggagaagcc ctggagaccg gcgaggctca
      361 gtggggtatg accctgggag aagctttccg gactaccaag tactgggtgc tgctgatctc
      421 catcttcgtg gtttacatgg cggtttccgg catcggcccc aacctcatcc cggcgctggt
      481 ggacgacggg ctgacgcctg cccaggcggc cacggcgctc agcacgttcg gcggcgcgat
      541 cattgccggg cggatcatcg tgggttacct cgtggaccgg ttctgggcgc caggagtggc
      601 ggccctgtcc ctcagccttc ccgtggccgg ctgcctgatg ctggtgggcg cccccggctt
      661 cgagaccgcc gtggccgccg cgctgctgat cgggatcgcg gcgggtgcag agcttgatct
      721 gatgtcgttt ctggcggcga aatacttcgg tctgcgtcac tacgccaaga tttacgcggt
      781 gctatatgcc accctggccg tctgctcggg cacggctccc atgctgtttg cccgggtgta
      841 cgacgtgacg gccagctacg acgtgagctt ctggatctcc acggttctgt tcgcgctggg
      901 cgccgtgctc gtcctagcca tgggcgcgta tccgcggatg ggggattcac gggaactgcc
      961 ctgatccgct ccgatgcagg gcgcgtagcg tggtgtcagg gcgagggttg gcgacatcta
     1021 ctttctgcac ggtgtcttga accgatactc cgggctccat tggctcgtgg tcggctcgcc
     1081 gaagctcagg tagtgggcgc gaaccttcca gctgtaggtt cgacaggcct ccagggagtc
     1141 cgggggcacg tagcgcattt tgtgggttcg aaagctcacc ggattggcct gtcggtcggc
     1201 cgcgatggcg agctcgtacg cgacagcttg ggcgggtgtg ccgttcccgc cgtccagagc
     1261 cgaccagacg aacatgggcc gccgcccgga gatcgtgcca gcgccgaatc gtcggcgcga
     1321 gacgggctcg acgccgtgga cccggatggc ggggtagaga aagaggtcgt ccgccatctc
     1381 tctggcgatg tcggcaaact gctcgtgcag ggcattggtc aggacgctgc cgccgtcacc
     1441 ggcccaatcg gaaaccgccc ggggcggaag ctcccgccag tactttctga tggcgagcga
     1501 ctggccgctt acgaggctgg tcagggtcag acgattcagg gtgtcgaaat agacgtggaa
     1561 gtcgtttgcg ctcacgtggc tggcgatgct gtccatgtcc atgtccagca cggagtcgaa
     1621 gccgcgcgcg gcgagctccg cgttgctggc atcggggtcg acgaattccc aatccatggg
     1681 cgccgacgtc gtcaactcgt tctccagtgc ccgctgcatc tgcgcgggcg cgaagtccag
     1741 cgtccgaacg agcacctcgt ttccggaagt gatctcttcc ttggaatcag cggctgcggc
     1801 cccgtagacg gcccctccgg cggcgacgat gggggcggtg acgagcccga aggcggcgat
     1861 cagcaatcct ccctcaccgg cttgacctgc cgcttcgaag gatccgccca gccaggttgc
     1921 ccccgccgag agcgccgttt tgcgtgctgc cgcgcccttc gtgtccacgt tcgccgccag
     1981 cgccaccttg ggacgatccg ggctgcgcac ggccatgcgg ccgatctggt cccgggtgat
     2041 gttcgcgatc cccgtgggct gaacgggttg ctggaccgcg cagcctccgg ccagaagcac
     2101 gatgaggacg gcggcggctt gggttcggat ctgcatctgg ctgtctccct gcgtgtacgt
     2161 ggcaggcagc ctcgcatcgc cgcgcgcgca gatcctgatg tgcggctgaa gcttgttgtg
     2221 gttttcttag gcgccggtaa ctatttgatt tatttatatt taaatttggc cttgcgcccg
     2281 taaacacgtg gacgtcgaag cgccacgtcg agccgtcagg gcatcggggt tggcggttcc
     2341 cagcccagat ccagaaagat ccattccagc aggtcggcca acgaccgctc ctttcgcagc
     2401 gtgccggacg cctgggccac gtgctcgcgg atcaggggct cgatgggccg gaaccaacca
     2461 taggtgagcc ggttgcgggg attcaccctg gggtgatgct tcaacagatc ggcgaaggca
     2521 tcctgagcct caggaatgcg gtctttcagg tagagcagca tcggcagctg cacctgaccg
     2581 tatgtttcgg tgccctccac ctgaagcgcc cgccgggcca gctggatcgc ctgctcgagg
     2641 tcgttgtcca tggccgctac caggctcagg gtagaccagg acatggaacc gaaacggctg
     2701 agggggtcca gtcggatggc cagctcggcc tcgcgccggg cctcttccag cttgccctga
     2761 tgagtcagca gggtcgcgta gttcgtgcgc aggatggcgt tgctcgggtt gacgggcaga
     2821 acggtatcga acaccgcctc ggcctgttcg atctcgccgc gcatgacgcg cacgaacccc
     2881 aggcagccgc acagcgcgcc gtcgaacggg ctgatgtcca gggcttcctg gaagtcctcg
     2941 aggatctcgt cctccatggc gtccgccgac tgcccggtga ggccgcgctc gacgagctga
     3001 tacaccttta tttccgccct tgcccagtac gcgagaggaa ggttcggatc cagctcgaga
     3061 gcccgctcgg catggagtag tccttcgtcg agccgatcga cgttcatggc gtttatgatc
     3121 tctaccgcgt tcacggcgtg cccccaggca tccaggctgg tgacgtcgcg cctcgctacc
     3181 cggtctattt ctccctcgag aatctgcggg tagctcacgc cggcgatctg ctcggcgatc
     3241 tggtcctgga gcgcgaaaac cgactcgggg tcgaagtcca cctcgtagga ctccttccag
     3301 agctggtgcc cgtcagcatc ggtcaggcca gcgctgactc tgagcctgtc gccccggcgg
     3361 ctgacggctc cggtcacgag gtagcgcgcg ccaagctggc tcgcgatctc ctggacggac
     3421 agccccttgt cccgcaacgc aaagctggca tgccgcgata tcaccgggat ggtgcgatat
     3481 tgctgcagac ctttgatgac ctcgtcggtc agtccgttgg cgaacgggcg atcctcgggg
     3541 tcgctgccgt ggtcgaacgg caacacggct atccttggtt gcccggcaaa cgtggtcacg
     3601 gcgaggcccg cgtcggacgg tgctcgaacc ggttcggtca cgaccatcca gaccagggca
     3661 atggcggcga gggcggttag ggccagtgct gcgaggagcg ggccgcgccg gtttggaggc
     3721 gatcgttgtc ggtgcgcctc gacttcggcg ggcgggtgcg gtttcccctg gaggtgactt
     3781 accagccttt cgaacgtcgc gtctgatcct tccggccgcc attgccgcag gtccactacc
     3841 tgagtcgtac ggaaagccac gggaagggta acgtcgtcga gaagaatcgg tatgagcgcg
     3901 tcgcgctcga gagcggccat cgactccgtg cgcacccacc gggaccgtat ggagttggcg
     3961 gtccagatcg ccacgatcca cgttgccgac tcgagctcgc gttcgatcgc ctcgtcccag
     4021 ctggtgccgg ggtggatctc gcggtcccac cacacggcga atctgcgttc ctccaacgcg
     4081 gttatcagcg gttttacgcg atcgaggtcc tcacttgcgt aggagacgaa gatgtccgcc
     4141 atgcatcccg cttccgccca ggtccggccg gcatgatcaa gtgaatggaa gcctagcaga
     4201 atgtgcactc gtcttcccgg acggcctttt cgctcccgcg tccactggga ttgtgcgctg
     4261 ggtcacaaac ttcccgcgcc tcagcgcaag gcgttttctc gatctaacgt gcagaatctt
     4321 caggcgcggt ggcctgtgcc acggattggc tgctcaagga aacgaaaccc gcaatgaaag
     4381 gaaaggtcct gttcttctcg ctggtgttct acgccgtcgt cggtatgtgg ctgtggggcg
     4441 agtaccagca aagacctcgc gtcctcgacg ggttggggca ggtgatgctt ggcatgacgc
     4501 cggcgcaggt ggtgggtgtt ctgggccggc cgaccgagga gtttgccccc gcggaggacg
     4561 aacagcggcg cctcgagtac tcctaccgca gtgatggtac gtccgattac ctcgtggttt
     4621 tctggccgga cgaaaagggc gagccgaagg ctgacatcat ctgtcgggaa ggcggcagcg
     4681 tcttcgggct ggccgtgggt cagtcttccg agctcgacat catcgacaag ctcggtgagc
     4741 cgtcccatcg cagcttcggc ccggacggcc gcgcaaaggc catctcttac gagagcttca
     4801 aggttgcctt cgagatcgag ggcggatgga cgcgggagat ctgcgtcagc caaacgggct
     4861 ccatcagcta cg
//
LOCUS       DBNHOX010000718         2335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_893378, whole genome shotgun sequence.
ACCESSION   DBNHOX010000718 DBNHOX010000000
VERSION     DBNHOX010000718.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_893378"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="AC16UZ_15580"
     CDS             <1..469
                     /locus_tag="AC16UZ_15580"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:NF012947.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR6248157.1"
                     /translation="AAIKRRRHKPMFIVDIAVPRDVEPEVAELPDVYLYTIDDLTEII
                     EENIKQRRSAAESAEHLVMEGAEAYTRERRAHEGQALLRSFRENAEAIQRAELEKALT
                     ALGRGGDPNVVLKSLSRSLTNKLIHQPTVAIRDASASDRSDLLEYLKSLYQLD"
     gene            479..1567
                     /gene="prfA"
                     /locus_tag="AC16UZ_15585"
     CDS             479..1567
                     /gene="prfA"
                     /locus_tag="AC16UZ_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR6248158.1"
                     /translation="MSASMMAKLSDLTDRFDEVSALLSDPETMADRDRFTALSKEYAE
                     LEPLILTFRRHDVLDTELREAQELAGDEDPEMRELAREDVDRLSKALDEEEQELQTLL
                     LPRDPSDASNVYLEIRAGTGGDEAALFAGDLFRMYGKFAESRGWSFEVLGARAGEHGG
                     YKEIITRVAGKDVYSQLKFESGAHRVQRVPETESQGRIHTSACTVAVMPEVDEVDSIE
                     INKSDLREDTFRASGAGGQHVNKTDSAIRLTHLPSGIVVECQDERSQHKNRARALALL
                     QAKLLDAAQSQQRSEQAETRRNLVGSGDRSERIRTYNFPQGRVTDHRINLTLYKLTEV
                     MEGALENVIDPLVREHHADQLRSLGDEQ"
     gene            1564..>2335
                     /gene="prmC"
                     /locus_tag="AC16UZ_15590"
     CDS             1564..>2335
                     /gene="prmC"
                     /locus_tag="AC16UZ_15590"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR6248159.1"
                     /translation="MTTVRAWIDRERELDLLDRHLLLAELLQTNRAGVMTYPERVLND
                     AELRTLDLWSSRRRHGEPMAYILGRKDFWGLTLRVNPTVLIPRPETELLVESALEVAA
                     AGQRLLDLGTGSGAVAIALARELANAAVSASDVSQAALKLAAANAAAHGARLTFIASH
                     WFENVTPSYDVIVSNPPYVAERDAHLTSLRAEPTAALVSGADGLDAIRYIVPAARRYL
                     NEAGWLIVEHGHDQGSSVRSLMRCSGYREVTTRRDLAGL"
BASE COUNT          437 a          777 c          769 g          352 t
ORIGIN      
        1 ggccgccatc aagcgtcggc gccacaagcc gatgttcatc gtcgacatcg ccgtgccgcg
       61 agacgtggag cctgaggtgg ccgagctgcc cgacgtgtac ctctacacca tcgacgatct
      121 caccgagatc atcgaggaga acatcaaaca gcgccgatcg gctgccgaga gcgccgagca
      181 tctggtcatg gaaggcgccg aagcctacac ccgcgagcgt cgcgcccacg agggccaagc
      241 gctgctgcgc agtttccgcg agaacgccga agccattcaa cgggcggagc tcgaaaaagc
      301 gctgacggca ctgggccggg ggggcgaccc gaatgtggtg ctgaagtcat tgagccggtc
      361 tctgaccaac aagctgattc accagccgac cgtcgcgatt cgcgatgcca gcgcgagtga
      421 ccgcagcgat ctcttagaat atctcaagtc tctctaccag ctagactgaa tcatgagcct
      481 gtccgcctcg atgatggcca agctgtcaga tctgaccgac cgcttcgacg aggtcagcgc
      541 cctcctgtcg gatcccgaga cgatggccga ccgggaccgg tttaccgcgt tgtccaagga
      601 gtacgccgag ctggagcccc tcatcctgac gttccgccgc cacgacgtgc tggacacaga
      661 gctgcgcgaa gcccaggagc tcgccgggga tgaggacccc gagatgcgcg agctcgcgcg
      721 ggaggacgtg gatcgcctgt caaaggcgct cgacgaggag gagcaggaac tgcagaccct
      781 gctgctgcca cgggacccca gcgacgcgtc gaacgtctac ctggagattc gcgcgggtac
      841 gggtggtgat gaggcggcgc tgtttgccgg ggacctcttc cgcatgtacg gcaagttcgc
      901 ggagtcccgc ggctggagct tcgaagtgct gggcgcccgg gcaggcgagc atggcggata
      961 caaggagatc atcacccgtg tcgccggcaa ggacgtctac agccagctga aattcgagtc
     1021 aggcgcgcac cgggtgcagc gcgttcccga aacggagtcc cagggacgca tacacacgtc
     1081 cgcgtgcacc gtggccgtga tgccggaggt ggacgaggtg gactccatcg agatcaacaa
     1141 gtccgatctg cgcgaggaca ccttccgcgc ctccggggcc ggcggacagc acgtgaacaa
     1201 gacggactcg gccatccggc tgactcacct gccgagcggc atcgtcgtgg agtgtcagga
     1261 cgagcgctcg cagcacaaga accgcgcgcg ggcgctggcg ctgctccagg cgaagctgct
     1321 cgacgccgcc cagtcgcagc aacgatccga gcaggcggag acgcgccgta atctggtggg
     1381 ctcgggcgac cgctcggagc gcatccgcac ctacaacttt ccccagggtc gcgtcaccga
     1441 tcatcggatc aacctgaccc tctacaaact gaccgaggtc atggaggggg cactggaaaa
     1501 cgtgatcgac cccctggtgc gtgagcatca cgcggatcag ctgcgcagcc tgggcgacga
     1561 gcagtgacca cggtccgtgc ctggatcgac agggagcggg agctggatct gctggatcgc
     1621 cacctgctgc tcgccgagct gctgcaaacc aaccgcgccg gcgtcatgac ctatccggaa
     1681 cgcgttctga acgacgcgga acttcgcacc ctggacctct ggtcgagccg tcggcgccac
     1741 ggcgagccca tggcctacat cctcggccgc aaggatttct gggggctgac gcttcgggtg
     1801 aacccgacgg tattgatccc ccgacccgag acggagctgc tggtggagag cgcgctggag
     1861 gtagccgcgg ccggtcagcg cctactggat ctcggcacgg gcagcggcgc cgtcgccatc
     1921 gcgctggccc gggagctcgc gaacgcggcg gtgagcgcaa gcgacgtctc ccaggcagcg
     1981 ctcaagctcg cggctgcaaa tgccgcagcc cacggcgcca ggctgacgtt cattgcgagc
     2041 cactggttcg agaacgtcac cccttcctac gatgtcatcg tcagcaaccc gccttacgtt
     2101 gccgagcgcg atgcccatct cacctcgctt cgggccgagc ccaccgccgc gctggtgagc
     2161 ggcgcagacg ggctggacgc cattcgctac atcgttcccg ccgccaggcg ctacctcaac
     2221 gaagccggtt ggctcatcgt cgagcacggc cacgatcagg gcagctcggt ccgttcgctc
     2281 atgcgttgca gcggctaccg cgaggtgacg acgcgacggg acctcgccgg gctag
//
LOCUS       DBNHOX010000719         6985 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1793048, whole genome shotgun sequence.
ACCESSION   DBNHOX010000719 DBNHOX010000000
VERSION     DBNHOX010000719.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6985)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6985
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1793048"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="AC16UZ_15595"
     CDS             <1..596
                     /locus_tag="AC16UZ_15595"
                     /inference="COORDINATES: protein motif:HMM:NF017319.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MlaC/ttg2D family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR6248160.1"
                     /translation="IDASRPYVEDDPERFYGEVESLLAPVVDFKRFARSVMAVHYKSA
                     TPEQRDRFADTFKWGLVRTYALALTEFRDGEVVIVPSERPPQSPRRESVKMEIRMNTG
                     DVYPVVYSMAQGKDGAWRMGNIIVNGVNIGLTYRSQFASAVQDQRYGGDLDKVIDAWT
                     LLLANETPGAASADSGGSDEADEAPSSDGAGGAGDDR"
     gene            593..883
                     /locus_tag="AC16UZ_15600"
     CDS             593..883
                     /locus_tag="AC16UZ_15600"
                     /inference="COORDINATES: protein motif:HMM:NF024857.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR6248161.1"
                     /translation="MSAFSLPPRVGVDNFAAVRAAGEQFLAERRDEKAVVDLSGLESC
                     NSIAVALLMTWFRFAHARGQEVVYAGVPQDLLSIIEISGLMEVLPLSRDAAG"
     gene            913..1164
                     /locus_tag="AC16UZ_15605"
     CDS             913..1164
                     /locus_tag="AC16UZ_15605"
                     /inference="COORDINATES: protein motif:HMM:NF013851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HIR6248162.1"
                     /translation="MQEEIAASITERLSGEDDALRVDVVVEGNRAMITVVSRMFEGMT
                     RVRKQQTVYGCIDAFIQDGRLHAVSIRSLTPEEAAGSSG"
     gene            1157..2422
                     /gene="murA"
                     /locus_tag="AC16UZ_15610"
     CDS             1157..2422
                     /gene="murA"
                     /locus_tag="AC16UZ_15610"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR6248163.1"
                     /translation="MDKLRITGGRTLSGSLRVSGAKNAALPILAGTLLADEPVTLSNV
                     PHLQDVTTMIELLGCMGARVLVDEKMRVEVSADSLAQLRAPYELVKTMRASFVVLGPL
                     LARFGEAEVSLPGGCAIGARPVDQHVKGLEALGAEISVNEGYVRGKVGGRLRGADFAF
                     DMVTVGATENLMMAAALAEGVTRLRNAACEPEVADLGDFLNAMGAKVSGHGSPEIRIE
                     GVPRLHGCEYRVMPDRVEAGTYLIAVAATGGRIRLTDVRPDTLTAVLDKLVESGAEVE
                     VGDDWIELDMHGRRPRAVDITTAPYPGFPTDMQAQYLAMNAIAEGTSEVSETIFENRF
                     MHVQELSRLGADIELHGPSSVSVRGVPSLKAAPVMATDLRASFSLVVAALVAEGTTLI
                     DRIYHIDRGYETIEEKLKLLGAEVQRVSG"
     gene            2438..3064
                     /gene="hisG"
                     /locus_tag="AC16UZ_15615"
     CDS             2438..3064
                     /gene="hisG"
                     /locus_tag="AC16UZ_15615"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR6248164.1"
                     /translation="MGLTIALNRGRILEESLPMLAAAGIVPEEDPAASRKLIFATRQG
                     GHRLVVMRGADVPTYVEYGAADVGIVGKDTLLEHGGSADYYERLDLGIAKCRIMVAAP
                     AGADLGSGKLRVATKFPRVARRYFEGQGRQVSIIKLNGAMEIAPLMNLADCIVDIVDT
                     GNTLRANGLEALETIAHVSSRMIVNRAAMKTRFDAVQSLTCSLRDVSG"
     gene            3061..4389
                     /gene="hisD"
                     /locus_tag="AC16UZ_15620"
     CDS             3061..4389
                     /gene="hisD"
                     /locus_tag="AC16UZ_15620"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR6248165.1"
                     /translation="MTEPALEIARLATGDADFPDRLARLLYWDTGLEDEVTRAAAEVI
                     EAVRAGGDDALLALTARYDRYECPSAAALEIAASELEAAYDALGSADREALNLAAQRI
                     REYHNHQLAGDWEYTDSDGNLLGQRVMALERVGLYVPGGQAAYPSTVLMTAVPARVAG
                     VDEIIMTVPTPDGVRSPLVLAAAHVAGVDRVFTVGGAQAIAALAYGTPTIPKVDKIVG
                     PGGAYVAAAKRLVFGTVGIDVIAGPSEVLVIADDTAPPEWLALDLFSQAEHDASAQAI
                     LVSPDPDVLDAVHDAMASLIGERSRATTIRASLAARGALIRAADLADAVALANRIAPE
                     HLELAVADPDALLPDVRNAGAIFIGAHSPEVMGDYVAGPSHVLPTFGTARFSSPLGVY
                     DFVKRSSVIRLSPGGSEKLARASATLARGEGLEAHARAAELRITGLDHNR"
     gene            complement(4407..5459)
                     /locus_tag="AC16UZ_15625"
     CDS             complement(4407..5459)
                     /locus_tag="AC16UZ_15625"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003115246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR6248166.1"
                     /translation="MRELVRYILVPALVGGAVGLGVMLTSQVNGTPTKPGYAAAVKRA
                     APGVVNIYSSKLVQHPMCEMPRFRDWCERFAGADRGQMQNSLGSGVIVREDGYILTNN
                     HVIEGADEIIVAFSDGQATTATLVASDPETDLAVIRVQASGLTPVPRDPSSVVEVGDV
                     ALAIGNPFGIGQTVSSGIISAKGRAGISRSPYEDFIQTDAAINPGNSGGALIDANGNL
                     IGINTLIFSRSGGSEGIGFAIPAQLAMAVLEEIIETGRVVRGYLGVELSSTPPAGAGV
                     GLEVTRLMSPSPAASAGLRPGDLILSINEQPAVSAAGVTRQIAHVEPGSELEIEVLRG
                     NRQISLTARAGERPQP"
     gene            5570..6670
                     /gene="zapE"
                     /locus_tag="AC16UZ_15630"
     CDS             5570..6670
                     /gene="zapE"
                     /locus_tag="AC16UZ_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HIR6248167.1"
                     /translation="MSPLQKYEEQLTRSGFFDDPAQREVAGLLDDLYRAFMARERLHA
                     SRLRRALRVLGIGAEPEPVRGLYLWGTVGRGKTLLMDLFYECLPGDVRLRMHFHRFMQ
                     RVHHQLTSLAGTPNPLQQVAERFASEASVLCFDEFAVSDIGDAMILAELLAELFRRGV
                     TLVATSNVEPDNLYENGLQRRRFLPAIELIRQHTVIHQVGGDVDYRLRVLERAEIYHV
                     PLDAGAERSLARSFDALAPEAPRDDVDLEIEGRMIRARRVAEDVAWFNFSDLCEGPRS
                     QNDYIELSRIFHAVLISGVPRFNTRSEDAARRFISLVDEFYDHGVKLIVSAEAEPASL
                     YGGERLRFEFERTVSRLLEMQSHDYLARSHRA"
     gene            6753..>6985
                     /gene="rplM"
                     /locus_tag="AC16UZ_15635"
     CDS             6753..>6985
                     /gene="rplM"
                     /locus_tag="AC16UZ_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR6248168.1"
                     /translation="MKTVSARAQDVQRSWLLVDAEDKTLGRLATEVARRLRGKHKPEY
                     TPHVDTGDYVVVINADKVRVTGNKTTDKIYYRH"
BASE COUNT         1187 a         2220 c         2394 g         1184 t
ORIGIN      
        1 tcatcgatgc atccagaccg tacgtcgagg atgacccgga acggttctac ggcgaggtgg
       61 agtcgctgct cgcgccggtg gtcgacttca agcggtttgc ccgcagcgtc atggccgtgc
      121 actacaaatc cgccactccc gagcagcgcg atcgtttcgc cgataccttc aaatggggcc
      181 tggttcgaac ctatgcgctg gcgctgacgg agttcaggga cggcgaggtg gtcatcgtgc
      241 cctccgagcg gccgccccaa agcccccgcc gcgagagcgt gaagatggaa atccgcatga
      301 acaccggaga tgtatacccg gtggtttact caatggcgca gggcaaggac ggcgcctggc
      361 gcatggggaa catcatcgtc aacggcgtga acatcggtct gacctaccgc agccagtttg
      421 ccagcgccgt gcaggatcag agatacgggg gggacctgga caaggtcatc gacgcctgga
      481 ctttgctcct ggccaacgag acaccgggtg ccgcgtcggc tgactcgggc gggtcagacg
      541 aggcggacga ggcgccgtcg agcgacgggg ccggcggagc tggtgacgat cgttgagcgc
      601 gttttccctg ccgcctcgcg tcggcgtcga caacttcgct gcggtcaggg cggctggcga
      661 gcagtttctc gccgaacggc gcgacgagaa agccgtcgtc gatctttccg gcctggagag
      721 ctgtaacagc atagccgtcg cccttctgat gacctggttt cgctttgccc atgcgcgggg
      781 gcaggaggtg gtttatgctg gcgtccccca ggatttgctc agcatcattg aaatttccgg
      841 gctgatggaa gttctgccgc tttcccggga cgcggcaggc tgaaccttta accgactgcg
      901 gcagggcggg acatgcagga agaaatcgcg gcgagcatca cagaaaggct gtccggggag
      961 gacgacgcct tgcgcgtgga cgtcgtcgtc gaggggaacc gcgcgatgat caccgtggtc
     1021 agccggatgt tcgagggcat gacgcgcgtt cgcaagcaac agaccgttta cggatgcatc
     1081 gatgctttca tccaggacgg tcgcctgcac gccgtgagca ttcgctccct cacgcccgag
     1141 gaggcggcgg gaagcagtgg ataagctccg cataacgggc gggcgcacgc tgtccggctc
     1201 gctgcgggtc tctggcgcaa aaaacgcggc gctgccgatc ctggcgggca cgcttctggc
     1261 cgacgagccg gtgacgttga gcaacgtccc tcatctccag gacgtcacca ccatgatcga
     1321 gctgctcggc tgcatgggcg cccgcgtgct cgtcgacgag aagatgcgcg tcgaggtttc
     1381 ggccgacagc ctggcgcaac tgcgggcgcc ctacgagctg gtgaaaacca tgcgcgcctc
     1441 gttcgtcgtg ctcgggccgc tgttggcgcg cttcggcgaa gcggaggtct cccttccggg
     1501 cggttgcgcc atcggggccc gccccgtgga ccagcacgtc aaaggcctgg aagccctcgg
     1561 tgcggagatc tccgtgaacg agggttacgt tcggggcaag gtcggcggcc ggctccgcgg
     1621 ggccgacttc gcgttcgaca tggtgacggt gggcgcaacc gagaacctca tgatggcggc
     1681 cgcgctggcc gagggggtca cgcggctgcg caacgccgcc tgcgaaccgg aagtggccga
     1741 cctcggagat tttctcaacg ccatgggcgc gaaggtttcg ggtcacggct ccccggaaat
     1801 aagaatcgaa ggcgtgccgc ggttgcacgg atgcgagtac cgggttatgc ccgatcgcgt
     1861 cgaagccggg acttacctga ttgccgttgc ggccaccggg ggcaggataa ggctcaccga
     1921 cgttcgaccc gacaccctta cggccgtgct cgacaagctt gtggaaagcg gcgccgaggt
     1981 cgaagttggc gacgactgga tcgagctcga catgcacggc cggcgaccgc gcgcggtgga
     2041 tatcaccacg gcgccatatc ccggctttcc gacggacatg caggcccagt acctggcgat
     2101 gaacgccatc gccgagggaa ccagcgaggt ctcggaaacc atcttcgaga accgcttcat
     2161 gcacgttcag gagctctcgc gactcggcgc ggacatcgag ctgcacggcc cgtccagcgt
     2221 ctcggtgcgc ggcgtgccgt cgctgaaagc cgctcccgtc atggccacgg acttaagagc
     2281 gtcgttcagc ctggtggtcg ccgcgctcgt tgcggaaggc accacgctca tcgataggat
     2341 ctatcacatc gaccgcggct acgagaccat cgaggagaag ctcaaactgc tcggcgctga
     2401 ggtccagcgg gtttcgggct gatcgagagg aattgaaatg ggtctgacca tagcgctcaa
     2461 ccggggcagg atcctcgagg agtccctgcc aatgctggct gcggcgggca tcgtcccgga
     2521 ggaggacccg gccgccagcc gcaagctcat ttttgccacc cggcagggcg ggcatcgcct
     2581 ggtggtgatg cgtggtgccg atgtccccac ctacgtggaa tacggggcgg cggacgtggg
     2641 catcgtgggc aaggacaccc tgctggagca cggcggcagc gcggattact acgagcgcct
     2701 ggacctgggc atcgcgaagt gccgcatcat ggtggctgct cccgcaggcg cggatctggg
     2761 atccggcaag ctgcgggtgg cgacgaagtt tccgcgggtg gcccgtcgct acttcgaagg
     2821 tcaggggcgt caggtatcca tcatcaagct aaacggcgcc atggagatcg ccccgctcat
     2881 gaacctggcg gattgcatcg tggacatcgt ggacaccgga aacaccctgc gcgcgaacgg
     2941 cctggaagct cttgagacca ttgcgcacgt ctcctcgcga atgatcgtaa accgggcggc
     3001 catgaagacc cgcttcgacg ccgtgcagag cctcacctgc agcctgcggg acgtgtcggg
     3061 atgacggaac cggcgctcga gattgcccgg ctcgcgacgg gcgatgcgga cttcccggat
     3121 cgtctcgcca ggctccttta ctgggacacg ggcctggaag acgaggtgac gcgagcggcg
     3181 gccgaggtca tagaagcggt gcgggccggt ggtgacgacg ccctgctggc gctcaccgcc
     3241 aggtacgatc gttacgagtg cccgagcgcc gcggcgctgg agatcgcggc ttccgagctc
     3301 gaggcagcct acgacgccct gggctcagca gaccgcgagg cgttgaacct ggccgcccag
     3361 cggattcgcg aatatcacaa ccaccagctg gccggagact gggaatatac cgacagtgac
     3421 ggcaacctcc tcggccagcg ggtgatggcg ctggagcggg tgggcctcta cgtgcctggc
     3481 ggccaggcgg cgtacccgtc aacggtgctg atgacggcgg tgcccgcccg ggtcgcgggt
     3541 gtggacgaga tcatcatgac cgtccccacg ccggatggcg tgcgttcgcc gctggtgctc
     3601 gccgcagccc atgtagcggg cgtcgaccgg gtatttaccg ttggcggcgc ccaggccatc
     3661 gccgcgctgg cctacggcac gccgacgatt cccaaggtgg acaagatcgt cggtcccggc
     3721 ggggcatacg tcgctgccgc caagcgcctg gttttcggca ccgtcggtat cgacgtcatt
     3781 gccggcccca gtgaggtgct tgtgattgcg gacgacacgg cgccgcccga gtggctcgcc
     3841 ctggacctgt tttcccaggc ggagcacgat gcttccgccc aggccattct ggtcagcccg
     3901 gacccggacg tgctcgatgc cgtccacgac gccatggcct cgctcatcgg cgagcggtcc
     3961 agagcaacga ccatccgagc ctcgctcgcc gcgcgaggtg ccttgatccg ggcggcggac
     4021 ctcgccgacg ccgtggcgct ggccaaccga atcgcgcccg agcatctcga gctcgccgtg
     4081 gcggatccgg acgcgctcct gcccgacgtt cgcaacgccg gcgcgatctt catcggcgcg
     4141 cactctccgg aggtgatggg cgattacgtg gcgggtccga gccacgtgct gccgacattc
     4201 ggtacggcca ggttctcgtc accgctgggg gtctacgatt tcgtcaaacg cagctccgtg
     4261 atccggctga gtccaggcgg gtccgaaaag cttgcccgcg cctcggcgac cctcgctcgg
     4321 ggcgaggggc tcgaagccca cgccagggcc gctgaattga ggatcaccgg gctggaccac
     4381 aaccgttagc cggacccgtg aggctcctag ggctgagggc gctccccggc gcgcgccgtg
     4441 agggagatct ggcgattccc acgcagaacc tctatctcca gttcgctgcc cggctcgacg
     4501 tgagcgatct gccgcgtgac gcctgccgcg ctcacggccg gttgctcgtt gatggaaagg
     4561 atcagatcgc ccgggcgcag accggccgaa gcggcggggc tgggtgacat cagtctggtg
     4621 acttccaggc cgacgcctgc cccggcaggc ggcgtgctgg acagctcgac gccgagatag
     4681 ccgcggacca cgcggcccgt ctcgatgatc tcctccagca ccgccatggc aagctgtgcg
     4741 gggatggcga acccgatgcc ctcggagccg ccggagcggg aaaagatcag cgtgtttatg
     4801 ccgatcaggt tgccgttggc atcgatgagc gcgcccccgg agtttccggg gttgatggct
     4861 gcgtcggtct ggatgaagtc ctcgtagggg ctgcggctga tgcccgcccg gccctttgcg
     4921 ctgatgatgc cgctcgagac cgtctggcca atgccgaacg ggttgccgat ggccagcgcg
     4981 acgtcgccca cctcgacgac gctgctcgga tcacggggca ccggcgtcag gcccgaggcc
     5041 tgcacgcgga tgacggcgag atcggtctcg gggtcgctgg cgaccagggt cgccgtggtg
     5101 gcctgaccgt cagagaacgc cacgatgatc tcgtcggccc cctcgatcac atgattgttg
     5161 gtgagaatgt agccgtcctc ccggacgatg acgccggatc ccagcgagtt ctgcatctgc
     5221 ccgcgatcgg cccccgcgaa gcgctcgcac cagtctcgaa accggggcat ctcgcacatg
     5281 ggatgctgta ccagcttgga cgagtagata ttgacgacgc cgggcgcggc gcgctttacc
     5341 gcggctgcgt acccgggctt ggtcggcgtt ccgttcacct ggctggtgag catgaccccg
     5401 agaccgacgg cacccccgac cagtgcaggg accaggatgt agcgaaccag ttcgcgcata
     5461 ggcggcggac gttaacagaa aaactcctgc cgcgccccgc ggcggcacgc ctcgcccgtc
     5521 gggtggcgcc gattgcgccg ttcctgaccg agcaagtaaa gttccgccga tgtcgccgtt
     5581 gcagaaatac gaagaacagc tcacgcgtag cggcttcttt gacgacccgg cgcagcggga
     5641 ggtcgccggg ctgctggacg atctctaccg ggcgttcatg gcccgggaaa ggctgcacgc
     5701 cagccggctg cggcgcgccc tgcgcgtgct gggcatcggg gctgagcccg agcctgtgag
     5761 gggcctgtat ctgtggggta cggtcggtcg aggtaagacc ctgctcatgg acctcttcta
     5821 cgagtgcctt cccggggacg ttcgtctgcg catgcacttc caccggttca tgcagcgcgt
     5881 tcaccatcag ctgacgagcc ttgcgggcac gccgaaccct ctgcagcaag ttgcagagcg
     5941 gtttgccagc gaggcaagcg tcctctgctt cgacgagttc gccgtttcgg acatcggcga
     6001 cgccatgatt ctggcggaac tgctggccga gctgttcagg cgtggcgtga cgctggtggc
     6061 gacgtccaac gtggagcccg acaaccttta cgagaacggc ctgcagcgcc gtcgttttct
     6121 gcccgccatc gagctcatca ggcagcacac cgtgatccat caggtcgggg gagacgtgga
     6181 ttatcgcttg agggttctgg agcgggcgga gatctatcac gttccgctgg acgccggggc
     6241 ggagcggagc cttgcccgga gcttcgatgc cctggcgccg gaagcgcccc gagacgacgt
     6301 ggatctcgag atcgaaggtc ggatgatcag ggcgcggcgg gttgccgagg acgtggcctg
     6361 gttcaacttt tccgatctct gtgaaggtcc ccgcagccag aacgactaca tcgagctctc
     6421 gcggatcttc cacgccgtgc tgatcagcgg ggtgcctcgc ttcaacaccc gatcggaaga
     6481 cgccgcgagg cgtttcatca gcctggtgga cgagttctat gatcacggcg tcaagctgat
     6541 cgtctccgct gaggccgagc ccgcctcgct ctatggggga gagcgcctgc ggttcgagtt
     6601 cgagcgcacg gtgagccggc tgctggagat gcagtcccac gactatctgg cccgctctca
     6661 cagggcctga gaaggcccgt cgggctggtt gtaaagggtc aggccctccg ctaacattcg
     6721 cgctcccaaa atcccggcat cttgaagggc tcatgaagac ggtaagcgcg cgggcgcagg
     6781 acgtgcagcg ttcgtggcta ttggtggacg cagaagataa gacgcttggt cgcctggcaa
     6841 ccgaggtggc tcgccgtctc cggggaaagc acaagccgga atacactcct cacgtggaca
     6901 cgggggatta cgtcgtggtc atcaacgccg acaaggtgcg ggtgacgggg aacaaaacca
     6961 ccgacaagat ctactaccgc cacag
//
LOCUS       DBNHOX010000720         4206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_765088, whole genome shotgun sequence.
ACCESSION   DBNHOX010000720 DBNHOX010000000
VERSION     DBNHOX010000720.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_765088"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="AC16UZ_15640"
     CDS             complement(<1..616)
                     /locus_tag="AC16UZ_15640"
                     /inference="COORDINATES: protein motif:HMM:NF015699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR6248169.1"
                     /translation="MTAFARVQGSAKGLSLIWEMRSVNHRFLETQFRLPEQMRPLEHG
                     LRETLRRHVKRGKFDCSLKIDRSTGGDGIELNRPLLLQLLAAVEQIRRDAPEIGPGNA
                     MDVLRWPGIIGDASTLEDEPLVQAATDLFEEALVQLIDNRQREGAQLASVISERLDEI
                     DSIAREVKALTATMSHEVRSRLTQRLNDLKVQADEGRLDQEVALL"
     gene            771..1490
                     /gene="rph"
                     /locus_tag="AC16UZ_15645"
     CDS             771..1490
                     /gene="rph"
                     /locus_tag="AC16UZ_15645"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIR6248170.1"
                     /translation="MTETQVQRPSGRAPSEMREVRFTRGFTKHAAGSVLVEFGDTKVI
                     CTASVENSVPGFLRGKGVGWVTAEYGMLPGATHVRTDREAARGKQGGRTQEIQRLVGR
                     ALRASVDMQALGERTIRLDCDVIQADGGTRTASITGSALALADAIASAGCNGALKELV
                     ASVSVGLWRGQPVLDLDYAEDSTAETDMNVVMLESGGFIEIQGTAEAGAFSDSELMSM
                     LALARSGTAELLRRQRQALSS"
     gene            1487..2155
                     /gene="pyrE"
                     /locus_tag="AC16UZ_15650"
     CDS             1487..2155
                     /gene="pyrE"
                     /locus_tag="AC16UZ_15650"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR6248171.1"
                     /translation="MSASRNDRSLVAFLIEQGVLAFGDFTLKSGRRSPYFFNLGALNT
                     GSALARLGAAYAERIRRLDVPFDVVFGPAYKGIPIAVSTAEALAALGGDVGWAFNRKE
                     AKSHGEGGLFVGADVSGRVMLVDDVLTAGTAVREAAALIGSAGAEIAGVVIALDRQER
                     VAPNSGETAVSALELELGAPVVSILKLQDVIEYLDLDVDRDKHRAELIREMKRYRDEH
                     CVLN"
     gene            2251..2856
                     /locus_tag="AC16UZ_15655"
     CDS             2251..2856
                     /locus_tag="AC16UZ_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248172.1"
                     /translation="MVAILLMPSWASANRLYRYLDASGHIAISHTIPSDRVKFGYEIV
                     DAGSGRVIEVVEAQKSPEEIARLEQEAAARKACEDALERVYSVYQSELDIENAEEQML
                     ESLEQRIANAEFNLERAQSQKDGFEATAARLERSGKELDTSLIANIERSQVQVRSLET
                     EIVQRRHEQAKAKERFARDRALFRQEICVEQTAISAVTETN"
     gene            complement(2881..3465)
                     /gene="slmA"
                     /locus_tag="AC16UZ_15660"
     CDS             complement(2881..3465)
                     /gene="slmA"
                     /locus_tag="AC16UZ_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HIR6248173.1"
                     /translation="MPPRSGRRDEILSAFARMLESNPGTRITTAALAGQIGVSEAALY
                     RHFPSKAKMIEGLIEFIEESLFSRINRILAEEASAASRCRAVLWLLLSFAERNPGLAR
                     LLVGDALQGETQRLRARMRQVFERLETELRSIIRDWSAARVPAPTLGATTAANLLLAS
                     AEGRISQFVRSEFRALPTSGWEEQWKALEGAVFG"
     gene            complement(3455..>4206)
                     /gene="argB"
                     /locus_tag="AC16UZ_15665"
     CDS             complement(3455..>4206)
                     /gene="argB"
                     /locus_tag="AC16UZ_15665"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR6248174.1"
                     /translation="VGLNPIIVHGGGPQIGELLDRLNIPTRFVKGMRVTDAETMDVVE
                     MVLGGLVNQDIVSLINKNGGRAVGITGKDGDLIRAHRLALSADAAELAPSEIIDIGHV
                     GEVESVNPAVLDALVRSNFIPVIAPIGVGPDGESYNINADLVAGRLAEELRAEKLILL
                     TNTAGILDANGQTLTSLSVAEINELIAAGTIGSGMLPKAQCAVSAAERGVGSTQIIDG
                     TIPHALLLEIFTDAGIGTQITTAEASGSDAS"
BASE COUNT          720 a         1311 c         1408 g          767 t
ORIGIN      
        1 ccagcagggc gacttcctgg tccaaccggc cttcgtcggc ctgaaccttc agatcgttga
       61 gtcgttgggt cagcctggag cgcacctcgt gactcatcgt cgcggtcagc gctttgacct
      121 cgcgagcgat gctgtcgatc tcgtccagcc gctcggagat gacggaggcg agctgcgctc
      181 cctcccgttg ccgattgtcg atgagctgga cgagcgcttc ctcgaacaga tcagtagcgg
      241 cctggacgag gggttcgtcc tccagagttg acgcatcgcc aatgatgccc ggccagcgca
      301 ggacgtccat ggcgttgccg ggccctatct ccggagcgtc ccggcggatc tgctcgaccg
      361 cggcgagcag ttggaggagc agcgggcggt tgagctcaat accgtcgccg ccggtgcttc
      421 ggtcgatctt cagcgagcag tcgaacttgc cgcgcttgac gtgacgccgc agggtttcac
      481 gcaggccgtg ctccaacggg cgcatctgct cgggaagccg gaactgggtt tccagaaaac
      541 ggtgattcac tgatcgcatc tcccagatca gcgagagccc cttggcgctg ccctggacgc
      601 gggcgaatgc ggtcatgctg tggatcatcg gtaggtcagc ggcacgtaaa atggcgagcg
      661 cgcattctac ccgggcgggg gccctgccat ccaaccgcgc cgcgcgatcg cgttgctgcc
      721 ggccgttgcc tttataatgc ccgcccactt ctgccgtccc gaggattttg atgaccgaaa
      781 cacaggttca gcgtcccagt ggccgggcgc cctcagagat gcgggaagtc cgcttcaccc
      841 gcggattcac caagcatgcc gcgggctccg tcctcgtgga gttcggtgat accaaggtga
      901 tctgtacggc cagcgtggag aactcggtgc ccggcttcct gcgaggcaag ggcgtgggct
      961 gggtgactgc cgagtacggc atgctccccg gcgccaccca tgtgcgaacc gatcgagagg
     1021 cagcgcgtgg caagcagggc ggtcgcacgc aggaaataca gcggctcgtc gggcgcgcct
     1081 tgcgggcgtc cgtggacatg caggcgctcg gcgagcgcac catccggctg gattgcgacg
     1141 tgatccaggc agacggcggc acgcggacgg catccataac gggctccgcc ctggcgctgg
     1201 ccgacgccat cgcctcggcc ggttgcaacg gggcgctcaa ggagctggtt gcctcggtgt
     1261 cggttggtct gtggcgcggt caacccgtgc tggatctgga ttacgccgag gactccacgg
     1321 cggagaccga catgaatgtg gtgatgctgg aatccggtgg gttcatcgag attcagggaa
     1381 cggccgaggc gggggcgttc agcgacagcg agctgatgag catgctggcc ctggctcggt
     1441 cgggcacggc ggagctgctg cgccgccaac ggcaggcgct gagctcttga gcgcctcccg
     1501 gaacgatcgc tccctggtgg cgtttctcat cgaacagggc gttctcgcgt tcggggattt
     1561 cacgctgaaa tccggccgca gaagccccta cttcttcaac ctgggggctc tgaatacggg
     1621 ttcagccctg gcgcggctgg gcgccgccta cgccgagcgc attcgccggc tggacgtgcc
     1681 gttcgacgtg gtgttcggtc ccgcttacaa gggcattccc attgcggttt ccactgcgga
     1741 ggcgcttgct gctcttggtg gcgacgttgg ttgggcattc aacaggaagg aagccaagtc
     1801 ccatggggag ggcggcctct tcgtaggcgc agacgtctcg ggcagggtca tgctcgtgga
     1861 cgacgtgctt accgcgggta ccgccgtgcg ggaagcggcc gcgctcattg gatctgcagg
     1921 cgcggagatt gccggcgttg tcattgcgct tgacagacag gagcgcgtag ccccgaactc
     1981 aggggaaacg gcggtttctg ccctggagct ggagcttggc gcaccggtag tcagcattct
     2041 caagctccaa gacgtgatcg agtaccttga tctggacgtc gatcgggaca aacatcgagc
     2101 cgaactaatc cgcgaaatga agcggtaccg cgacgagcat tgcgttctga actgaaccgg
     2161 ctagtcgctc ataactcgac gattgagcga ttgatgggaa cacgataccg aggcaatttc
     2221 cgatgtcgcc cgcgggcggc ggtcttgatc atggtggcga tcctcctgat gccgtcctgg
     2281 gcttcggcca atcgcctgta ccggtatctc gatgccagcg gtcacatagc gatctctcac
     2341 accatcccca gcgaccgggt caagttcggc tacgagatcg tcgacgccgg cagcgggcgg
     2401 gtgatcgagg tcgtggaggc ccagaagagc cctgaggaaa tcgccaggct ggaacaggaa
     2461 gcggccgccc gcaaggcgtg cgaagacgcg ctcgagcgcg tctattcggt gtaccagtct
     2521 gagctcgata tcgagaacgc agaagagcag atgctggaat ctctagagca gcgcattgcg
     2581 aacgccgagt tcaacctcga gcgcgcgcaa agccagaaag acggcttcga ggccaccgct
     2641 gcccgactcg agcgctccgg caaggaactg gacacctccc tcatcgccaa tatcgagagg
     2701 tcccaggttc aggtgaggag cctggagacg gagatcgttc agcgccgcca tgagcaggcg
     2761 aaagccaagg aacgtttcgc ccgtgatcgg gctcttttcc gccaggaaat ctgtgtcgag
     2821 cagacggcca tcagcgcggt tacggaaacg aactgagggc gtttgccgtt ccttggctgg
     2881 ctagccgaag acggcccctt ccagagcctt ccactgttcc tcccagccgg aggtcggcag
     2941 cgcgcgaaat tcgcttcgaa cgaactggct gatgcgaccc tccgcactgg ccaggagcag
     3001 gttggcggcc gtcgtggcac cgagcgttgg tgccggcacc cgggcggccg accagtcccg
     3061 gatgatcgac cgaagctcgg tttccagccg ctcgaaaacc tggcgcatgc gcgcccgaag
     3121 ccgttgggtc tcgccctgca gggcgtcccc gaccagcaag cgcgccagtc ccggattccg
     3181 ctcggcgaag gaaagcagca gccacagcac ggcccggcag cgggacgcgg cgctggcctc
     3241 ctctgcgaga atgcgattga tgcggctgaa gaggctctcc tcgatgaact cgatgagccc
     3301 ctcgatcatt tttgccttgc tgggaaagtg ccggtagagg gcggcctcgg ataccccgat
     3361 ctggccggcc agggcggcgg tggttatccg cgttcccggg ttgctttcca gcattcgcgc
     3421 gaacgcgctg agaatttcgt cgcggcggcc cgatctagga ggcatcggac ccgctcgcct
     3481 cggcggtggt gatctgcgta ccgattccgg catcggtgaa gatttccagc aggagagcgt
     3541 gaggtatggt tccgtcgatg atctgtgtgc tccctacgcc gcgctcggca gcggacacgg
     3601 cgcactgcgc cttgggcagc atgcccgagc cgatggtgcc cgcggcgatg agctcgttga
     3661 tctccgcgac gctcagcgag gtgagtgtct ggccattggc gtccaggatg ccggcggtgt
     3721 tggtcagcag tatcaacttt tctgcgcgca gctcctccgc caggcggcct gcgacgaggt
     3781 cggcattgat gttgtaggac tcgccatcgg gccccacgcc aatgggcgcg atcaccggga
     3841 tgaagttgct gcgcacgagg gcgtccagga cggccgggtt gacgctttcg acctcgccga
     3901 cgtggccgat gtcgatgatc tccgacgggg cgagctccgc cgcgtcggcg ctcagcgcca
     3961 gccggtgcgc cctgatcaga tcgccgtctt tacccgttat ccccacggcg cgaccgccgt
     4021 tcttgttgat cagggatacg atgtcctgat tcaccagccc gccgagaacc atctccacca
     4081 cgtccatggt ctcggcgtcc gtgacccgca tgcctttgac gaagcgggtg ggaatgttga
     4141 gccgatccag gagctcgccg atctgcggcc cgccgccatg gacgatgatg ggattcagac
     4201 cgacca
//
LOCUS       DBNHOX010000721         3112 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_637782, whole genome shotgun sequence.
ACCESSION   DBNHOX010000721 DBNHOX010000000
VERSION     DBNHOX010000721.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_637782"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /gene="acs"
                     /locus_tag="AC16UZ_15670"
     CDS             <1..1749
                     /gene="acs"
                     /locus_tag="AC16UZ_15670"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetyl-CoA synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIR6248175.1"
                     /translation="ISWFAGGKLNIAYNCIDRHLPDRASQTAIIWEGDDPANDKTITY
                     QELHDAVCQLANGLRSRGVGKGDRVCIYMPMVPEAAYAMLACMRIGAIHSVVFGGFSP
                     QSLKDRILDSDCRTVITADHGVRGGRLVPLKENTEAALKDCPNVHTVVTVKRTGNEIP
                     WNPDRDIWYEDLVADQPRTAEPEVMDSEDPSFILYTSGSTGKPKGVQHGTAGYLLFAA
                     MTHKYVFDYHDGDIYWCTADVGWITGHSYIVYGPLANGATTIMFEGVPTYPDASRCWQ
                     VVDKHKVNIFYTAPTALRQLMGQGDEYVTQTSRASLKLLGTVGEPINPEAWQWYHKVV
                     GEERCPIVDTWWQTETGGIMISPLPGATALKPGSATRPFFGVELGLMDENGRLIEEQE
                     ASGNLVITNTWPGQIRSVYGNHQRVIDTYYSQYPGYYFTGDGARRDADGYYWITGRVD
                     DVINVSGHRIGTAEVESALVLHEAVAEAAVVGFPHPVKGEGIYAYVTLMVGNNEDPDQ
                     LRGELEALVRSEIGPFARPEFIQYAPGLPKTRSGKIMRRILRKIAADDLENLGDTSTL
                     ADPGVVDDLVHNRANR"
     gene            complement(1799..>3112)
                     /gene="pnp"
                     /locus_tag="AC16UZ_15675"
     CDS             complement(1799..>3112)
                     /gene="pnp"
                     /locus_tag="AC16UZ_15675"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR6248176.1"
                     /translation="MERQARVSELRAALVGDLGGGNEPSFASDNVAGAFGKVEKDIVR
                     QRVLDGEPRIDGRDLRTVRPISVEVGFLPKTHGSALFTRGETQAIVVATLGTMRDAAL
                     IDALEGTYKDNFMLHYNFPPYSVGEAGFMTGPKRREIGHGRLARRGVQAMLPDVDDFP
                     YTIRVVSEITESNGSSSMASVCGTSLALMDAGVPMKAPVAGVAMGLVKEGNKYAVLTD
                     ILGDEDHLGDMDFKVAGSANGICALQMDIKIDGITEEIMEIALDQAREARLHILGEMN
                     KVLDRSRETLSENAPSMVVLKVHPDKIRDIIGKGGATIRGIVDETGADIDIEDDGSVR
                     IYAEDAASKDAAVARIEEITAEAEIGKVYTGKVARIVDFGAFVNILPGKDGLLHISQI
                     AEERVENVTDYLQEGQDVQVVVLDIDQRGRIKLSMKEVANYAQSA"
BASE COUNT          580 a         1014 c          960 g          558 t
ORIGIN      
        1 atcagctggt tcgcgggcgg caagctcaac atcgcgtaca actgcatcga ccgacacctg
       61 cccgaccggg cgagtcagac ggcgatcatc tgggaagggg acgaccccgc caacgacaaa
      121 acgatcacct atcaggaact gcacgatgcg gtctgccagc tcgcaaacgg gcttcggtcg
      181 cgcggggtgg gcaaaggcga tcgagtctgc atctacatgc cgatggtgcc ggaggcagcc
      241 tacgccatgc tcgcctgcat gcgcatcggc gccattcact ccgtcgtgtt cggcggtttc
      301 tcgccgcagt ctctgaaaga ccggatcctc gactccgact gccggaccgt gatcacggca
      361 gaccatggcg tccggggcgg ccggctggtg ccgctgaagg agaacaccga ggcggctttg
      421 aaggattgtc cgaacgtcca cacggtcgta acagtcaagc gaacgggcaa cgagatcccc
      481 tggaacccgg atcgcgacat ctggtacgag gatctggtgg ccgatcagcc caggacggct
      541 gagcccgaag tgatggattc cgaagatccc tcgttcatcc tctacacgtc cggctccacc
      601 ggcaagccga aaggcgtaca gcacggcact gccggctatc ttctcttcgc ggccatgacc
      661 cacaagtacg tcttcgacta ccacgacggg gacatctact ggtgcacggc ggatgtgggt
      721 tggataaccg gacattccta catcgtctac ggtcctctgg ccaacggcgc cacgaccatc
      781 atgttcgagg gtgtgccgac ctatcccgac gcctcacgct gctggcaggt ggtggacaag
      841 cacaaggtca atatcttcta caccgcccca acggcgctcc ggcagctgat gggccagggc
      901 gacgagtacg tgactcagac gtcgcgggcg tcgctgaagc tcctcggcac ggtcggcgaa
      961 cccatcaatc cggaggcgtg gcagtggtat cacaaggtgg tgggcgagga gcgctgcccc
     1021 atcgtcgaca cctggtggca gacggaaacc ggcgggatca tgatctctcc cctgcccggg
     1081 gccaccgcgc tgaaacccgg ttcagccaca cggcccttct tcggggtgga gctggggctc
     1141 atggacgaaa acggccggct catcgaggaa caggaggcaa gcggcaacct ggtgattacg
     1201 aatacgtggc cgggccagat ccggtcggtg tacggaaacc accagagggt catcgacacc
     1261 tactactcgc agtatccggg ctactacttc accggcgacg gcgcgcgccg cgacgccgac
     1321 ggctactact ggatcaccgg ccgggtggac gacgtcatca acgtctcggg gcacaggatc
     1381 ggcaccgccg aggtcgaaag cgcgctggtt ctgcacgaag cggtggccga ggcagccgtg
     1441 gtcggctttc cgcatcccgt caagggagag ggcatctacg cctacgtgac gctgatggtt
     1501 ggcaacaacg aggacccgga tcagctcaga ggggagctgg aggcgctggt gcgctccgag
     1561 atcggaccct tcgcccggcc ggagttcatt cagtacgcgc ccggcctgcc gaagacccgg
     1621 tcgggcaaga tcatgcggcg catccttcgc aagattgccg ccgacgatct ggagaatctg
     1681 ggagacacgt ccaccctggc ggaccctggc gtcgtggacg atctggtaca caaccgggcc
     1741 aaccgctgag accaggctgc gccgagtacc ggctcagcct ggccttagcg ggtggggatc
     1801 aggcgctttg cgcgtagttg gccacttcct tcatggaaag tttgatgcgg ccgcgctgat
     1861 cgatgtctag aacgactacc tggacatcct ggccttcctg gaggtaatcg gtgacgttct
     1921 ccacacgctc ttcggcaatc tgagagatgt ggagcagccc atccttgcca ggcaggatgt
     1981 tcacgaacgc gccgaaatcg acgatgcggg ccaccttgcc ggtgtagacc ttgccgatct
     2041 ccgcctctgc ggtgatctcc tcgatccgcg caaccgccgc gtctttcgac gccgcgtcct
     2101 cggcgtaaat gcgcaccgag ccgtcgtcct cgatgtcgat gtcggcaccg gtttcgtcga
     2161 cgatgcctcg aatggtcgca ccgcctttgc cgatgatgtc gcggatcttg tccggatgca
     2221 ccttcaggac caccatgctc ggcgcgttct cggaaagcgt ctctcgagac ctgtccagga
     2281 ccttgttcat ctcgcccagg atgtgcaggc gtgcttcgcg cgcctggtcc agggcaattt
     2341 ccatgatctc ctcggtgatg ccgtcgatct tgatgtccat ctgcagcgcg cagatgccat
     2401 tggcggatcc agccaccttg aaatccatgt ctccgagatg atcctcgtca ccaagaatgt
     2461 cggtgagcac ggcgtacttg ttaccttcct tcaccagccc catcgccacg cccgcaaccg
     2521 gcgccttcat cggcaccccg gcatccatca gcgccaggga ggtcccgcag acgctggcca
     2581 tggagctcga gccgttagac tcggtgatct cggagacgac ccgtatcgtg taggggaagt
     2641 cgtccacgtc cggcagcatg gcctgcacac cgcgacgggc cagacgcccg tgaccaatct
     2701 cgcgccgctt cggccccgtc atgaagcccg cttcgccgac gctgtagggc ggaaagttgt
     2761 agtgcagcat gaagttgtct ttataggtcc cttcgagggc atcgatcagg gccgcgtcgc
     2821 gcatggtgcc tagcgtcgcc acgacgatcg cctgggtttc gcccctggtg aagagtgccg
     2881 agccgtgggt cttgggcaga aagccgacct cgaccgagat cggacgcacg gtgcgaagat
     2941 cccggccgtc gatgcgcggc tcgccatcga gcacgcgctg acgcacgatg tccttttcca
     3001 ccttcccgaa cgcgccggcg acgttgtccg aagcaaagct cggctcgttg ccaccaccga
     3061 ggtctcctac cagcgccgca cgcagctcgg aaacccgagc ctgtcgctcc at
//
LOCUS       DBNHOX010000722         4129 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1215326, whole genome shotgun sequence.
ACCESSION   DBNHOX010000722 DBNHOX010000000
VERSION     DBNHOX010000722.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1215326"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="AC16UZ_15680"
     CDS             complement(<1..581)
                     /locus_tag="AC16UZ_15680"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HIR6248177.1"
                     /translation="MKRMTSFRTRWPRWLAATSLTALAACATMPEQEPASRNVILFLG
                     DGMGVSTVTAARIFEGQRQGLAGEEHALAFEHFPHVALVKTYNTDGQVPDSAGTMSAI
                     MTGEKTRVGHIAVGPEVDNDDCPGSLAGKRQTLLEEAELRGYATGIVSTARITHATPA
                     ATYAHVPNRDWEADSMLPALASAAGCRDIAAQLV"
     gene            complement(611..1963)
                     /gene="gorA"
                     /locus_tag="AC16UZ_15685"
     CDS             complement(611..1963)
                     /gene="gorA"
                     /locus_tag="AC16UZ_15685"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HIR6248178.1"
                     /translation="MSYDYDLFVIGAGSGGVRAARMSAGYGARVAVAESTYLGGTCVN
                     VGCVPKKLFVYGSHFHEDFEDAAAYGWDVSPTGFDWPRLRDNKTREIERLNGVYANLL
                     EKAGVETKWGHARIAGPNTVAMDGQTFTAEHILIATGSWPAVPDVPGRELAITSNEAF
                     YLESFPKRVVVVGGGYIAVEFAGIFKGLGANVELLYRRDLFLRGFDDGVRQFVANELT
                     QKDIGLRFNTNVARIEAVDGRRRVHLVEGGSLDADLVLYATGRTPKTGDLGLETVDVA
                     LNERGGIVVDEFYRTTAPGIYALGDVIDNVQLTPVAIAEGMCVSANLFTDRPASRVNY
                     ENIPTAVFCQPNIGTVGPTETEARERYPDLAVFESEFRPMKHTLTGRQERTFMKLLVD
                     GASDRVVGAHMVGPEAGEIVQGIGVAITAGATKAHFDQTIGIHPTAAEEFVTMREPAR
                     "
     gene            2094..3638
                     /locus_tag="AC16UZ_15690"
     CDS             2094..3638
                     /locus_tag="AC16UZ_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HIR6248179.1"
                     /translation="MNGAQSLLKTFVNAGVEVCFTNPGTSEMHFVAALDTEDGMRCVL
                     GLFEGVVTGAADGYARMAGKPACTLLHLGPGLANGLANLHNARRANSPIVNVVGEHAT
                     YHRHLDAPLTSDIEGFASPVSGWTRVSESAASVAADGAEAVRASLQPPGQIATLILPA
                     DTAWNEPAAEADPLPSADPGPFDALAVEAAARALRSGEPCALLLNGVGVSEAGQILAS
                     RIAEHCGARLLGDTFIARIPGGAGRVTIPRLPYFAEQAEEALAGVRHLIMVDTKPPVS
                     FFAYPGRASELTPNGAEAIVLTQAGEDTIGALEALVDAVGATGQQPVLADLQRPDLPD
                     GPLNPETIARALAALLPEQAILVNEAATSGFGLPAFLPRTAPYDMLDLTGGAIGMGLP
                     AATGAAIACPDRRVVALQADGSAMYTVQALWTMAREGLDVTVVLFSNRKYAILQVEFM
                     RVGAHNPGPKAMDMLDLTRPDLDWVSIARGMGVDAVRADDAASFNQALGRSFATRGPF
                     LIEAVI"
     gene            3648..>4129
                     /locus_tag="AC16UZ_15695"
     CDS             3648..>4129
                     /locus_tag="AC16UZ_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HIR6248180.1"
                     /translation="MKITMFGTGYVGLVTGTCFAEMGNEVLCVDVNAEKIEALNAGQI
                     PIYEPGLEQMVKSNASGGRLKFSTDVEEGVHHGRVLFIAVGTPSDEDGSADLSYVIGV
                     AKTIGQHMAEPKVVVDKSTVPVGTADQVRVAIGETLAARGAEIGFDVVSNPEFLKEGA
                     A"
BASE COUNT          659 a         1414 c         1348 g          708 t
ORIGIN      
        1 accagctgcg ccgcgatgtc cctgcagccg gccgcggaag ccagggcggg cagcatgctg
       61 tccgcttccc agtcccggtt gggcacgtgg gcataggtgg cagccggcgt cgcgtgggtg
      121 atcctggccg tggagacgat gcccgtcgcg tagccgcgca gctcggcctc ttccaatagg
      181 gtctgacgtt tgccggccag agatcccggg caatcgtcgt tgtcgacctc cggccccacg
      241 gctatgtggc ctacgcgggt cttctccccg gtcatgatcg cggacatggt cccggcgctg
      301 tcgggaacct gaccgtccgt gttgtaggtc tttaccagcg ccacgtgagg aaagtgctcg
      361 aaagccagcg cgtgctcctc gccggcgagg ccctgacgtt gcccttcgaa tatgcgggcg
      421 gcggtgacgg tgctgacccc cataccgtcg ccgaggaaga gaatgacgtt ccggctggcc
      481 ggttcctgct ccggcatggt cgcgcaggcc gccagcgccg tgagggatgt ggcagcgagc
      541 catctcggcc atcgcgtccg gaaacttgtc atcctcttca tgccgtccgc tccgcttcct
      601 gcacccgcgg ctacctcgcg ggctcgcgca tggtgacgaa ctcctccgcc gccgtggggt
      661 gaatgccgat ggtctggtcg aagtgcgcct tggtcgcacc cgccgtgatg gcgacgccga
      721 tgccctggac gatctcgcca gcttccggac cgaccatgtg cgcaccgacg acgcggtcac
      781 tcgcaccgtc gaccagaagc ttcatgaacg tgcgctcctg ccggccggtc agcgtgtgct
      841 tcatgggccg gaactccgac tcgaacacgg caaggtccgg gtagcgctcc cgggcttcgg
      901 tctccgtcgg ccccaccgtg ccgatgttcg gctggcagaa cacggcggtg ggaatgttct
      961 cgtaattcac gcggctggcc ggccggtcgg taaacaggtt ggcagacacg cacatcccct
     1021 cggcgatggc taccggcgtc agctgcacgt tgtcgatcac gtcgccaagc gcatagatgc
     1081 cgggcgccgt cgtccgatag aactcgtcca ccacgatgcc gccacgctcg ttgagcgcaa
     1141 catctaccgt ttccaggccc agatcgcccg tcttcggggt cctgccggta gcgtagagca
     1201 ccagatccgc gtcgagagat ccaccctcca ccaggtgcac gcgccgccgg ccgtccaccg
     1261 cttcgatgcg ggccacgttg gtgttgaacc tcagaccaat gtccttctgg gtgagctcgt
     1321 tggcgacgaa ctgacgcacc ccgtcgtcga agccccgcag gaacagatcg cgccggtaga
     1381 gcagctcgac gttcgccccg aggcctttga atattccggc aaactccacc gcgatgtagc
     1441 cgccgccgac caccaccacg cgtttgggaa acgactccag atagaacgct tcgttggagg
     1501 tgatggccag ctcccggccg gggacgtcgg gcaccgcggg ccagctgcct gtggcgatca
     1561 gaatgtgctc ggcggtgaac gtctgcccgt ccatggccac cgtgttcggc cctgcgattc
     1621 gcgcatggcc ccatttggtt tccacgcccg ccttctcgag caggttcgcg tacacgccgt
     1681 tgagccgctc gatctcccgc gtcttgttgt cgcgcagcct cggccagtcg aatcccgtgg
     1741 gcgaaacgtc ccagccgtac gccgcggcgt cctcgaaatc ctcgtgaaag tgggagccgt
     1801 agacgaagag cttcttgggc acgcagccca cgttgacgca cgtgccgccc aggtaggtgc
     1861 tctcggctac ggccacccgg gcgccgtatc cggccgacat ccgcgcggct ctgacgccgc
     1921 cggatcccgc gccgatcacg aacaggtcgt agtcgtagct catcgtctcg attctccccg
     1981 gggaaacgga cccgcagatg cgggatgtga cggagcgtaa ttgatgagca ccgaattgca
     2041 atgcttcttc cggcccacat acactccgct ttcctctcgt caccggtacg gccatgaacg
     2101 gcgcgcagag cttgttgaag acgttcgtga acgcaggcgt ggaagtctgc ttcaccaatc
     2161 cgggaacctc ggagatgcac ttcgttgccg cgctggacac ggaggacggc atgcgctgcg
     2221 tgctcgggct gttcgaggga gtggtcaccg gagcggcgga cgggtacgcc cgcatggccg
     2281 gcaagccggc atgcacgctg ctgcatctgg gcccgggcct ggccaacggc ctcgccaatc
     2341 tgcacaacgc ccgccgggcg aactctccaa tcgtcaacgt ggtgggtgag cacgcaacct
     2401 accaccggca tctggacgcg ccgctgacct ccgacatcga aggcttcgcg agcccggttt
     2461 ccggctggac gcgcgtctcg gagtcagccg catcggtagc cgccgatggc gccgaggcgg
     2521 tgcgggcttc gctgcagccg ccggggcaga tcgccaccct catcctgccc gccgacacgg
     2581 cctggaacga acctgcggcc gaggccgatc ctcttccttc ggcagacccc ggaccattcg
     2641 atgccctcgc cgtggaagcg gccgcccggg cgttgcgctc cggcgagccc tgcgcgctgc
     2701 tcttgaacgg cgtgggcgtg agcgaggcgg gacagatcct ggccagccgc atcgccgagc
     2761 actgcggcgc gcggctgctg ggagatacct tcatcgcgcg gatccccggg ggcgccggcc
     2821 gggtcaccat cccgcgtctt ccctactttg ccgagcaggc cgaagaggct ctggccggcg
     2881 tccgccacct gatcatggtg gacacgaaac caccggtgag ctttttcgcc tacccgggtc
     2941 gcgccagcga gctcactccc aatggggcgg aggccatcgt gctcacccag gcgggagaag
     3001 acaccatcgg cgcgctggag gccctggtcg atgccgtggg ggcgaccggc cagcagcccg
     3061 tgcttgccga tctgcaacgg cccgatctcc ccgacggccc gctgaacccc gagaccatcg
     3121 cccgggctct tgccgccctg ctacccgagc aggccatcct cgtcaacgag gcggccacct
     3181 ccgggttcgg gctcccggcg tttctgcccc gaaccgcacc ctacgacatg ctcgacctga
     3241 ccggcggcgc catcggcatg gggctgcccg ccgctaccgg tgccgccatc gcctgtccag
     3301 accggcgggt cgtggcgctg caggcagacg gcagtgccat gtacacagtg caggctctgt
     3361 ggaccatggc acgggagggg ctggatgtga cggtggttct gttctcaaac cgcaagtacg
     3421 ccatcctgca ggtggagttc atgcgcgtgg gcgcccacaa tcccgggccg aaagccatgg
     3481 acatgctgga tctcacccga ccggacctcg actgggtgag cattgcccgg gggatggggg
     3541 tagacgccgt tcgcgctgac gatgcggcgt cgttcaacca ggcgctcggg cgcagctttg
     3601 cgacccgggg cccgtttctc atcgaagccg tcatttagaa tgaaatcatg aagatcacca
     3661 tgttcgggac cggctacgtg ggcctggtca ccggtacctg ctttgccgag atgggcaacg
     3721 aggtcctctg cgtggacgtg aacgcggaga agatcgaagc cctcaacgct ggacagattc
     3781 ccatctacga gcccgggctc gagcagatgg tcaagtccaa cgcctccggt ggccgtctga
     3841 agttcagcac agacgtcgag gaaggcgtgc accacggccg ggttctcttc atcgccgttg
     3901 ggacgccttc ggacgaggac ggctcggcgg acttgagcta cgtgatcggc gtggcgaaga
     3961 ccatcgggca gcacatggcc gagcccaagg tggtggtcga caaatccacc gtgcccgtgg
     4021 gcacggcgga tcaggtgcgc gtcgccatcg gggaaaccct tgcggcccgg ggggcggaga
     4081 tcggcttcga cgtggtctcc aatcctgagt tcctcaagga aggcgcggc
//
LOCUS       DBNHOX010000723         1594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1537748, whole genome shotgun sequence.
ACCESSION   DBNHOX010000723 DBNHOX010000000
VERSION     DBNHOX010000723.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1537748"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..700)
                     /locus_tag="AC16UZ_15700"
     CDS             complement(107..700)
                     /locus_tag="AC16UZ_15700"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR6248181.1"
                     /translation="MEIPLFPLRVVLFDGGRLPLQIFETRYLDMVSRCTRESIPFGVV
                     LIRGAGSDARLDPLANQPAIFEVGTEAHIVDFNQLPNGRLGIIVRGGRKFRVVSTREQ
                     ADHLLLGTVDFLPAEPDSTVPSEHAPLVDILKELVKHPGVQKLGLEIDFADARSVGAR
                     LAELLPIEPEIKQSLLQLQLPRERLQELTRLVNKFRD"
     gene            780..1568
                     /locus_tag="AC16UZ_15705"
     CDS             780..1568
                     /locus_tag="AC16UZ_15705"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR6248182.1"
                     /translation="MDWLQIIILALVQGITEFLPISSSAHLILPALLTEWPDQGLAFD
                     IAVHAGTLLAVLSYFRREIGRFAASGARLLARGQRDDDADLLLKLIAATLPIVLVGYL
                     AHDWVESSLRTVPVIASATIVFALALWWADGRRSGADHPSWTAAMLIGAAQVIAIVPG
                     TSRSGITMTAALLLGLSRTGAARFSFLLSIPTILGAAVLEAASLGAGEHAVRWAELVA
                     GAVLAGAGAYCCIHFFVKLVERTGMLPYVVYRLMLGTLLLGLAL"
BASE COUNT          227 a          510 c          542 g          315 t
ORIGIN      
        1 ggggcggtca gggcggccag ggcggcgggt tcgaatcagc tgcgcccgca gcgtccgaga
       61 acgcgccaac gctggcggac gacgacttcg acgacgacat tcccttctag tcccggaatt
      121 tgttgaccag ccgggtcagc tcctggaggc gttcccgggg cagctgcagc tgcagcaggc
      181 tctgcttgat ctcgggttct atgggaagca gctcggccag gcgggcgccc accgaccgcg
      241 cgtcggcgaa gtcgatttcc aacccgagct tctgcacccc gggatgtttc accagctctt
      301 tgaggatgtc gaccagaggg gcgtgctctg atggcacggt gctatcgggc tcggccggca
      361 ggaagtccac cgttccgagc agcaggtggt cggcctgctc ccgggtcgat accacgcgga
      421 atttgcggcc accccggacg atgatgccga gccgtccgtt cggcagctga ttgaagtcga
      481 cgatgtgggc ttcggtgcct acctcgaaaa tcgcgggctg gttggcgagc ggatccaggc
      541 gagcgtcgct gcccgcaccc cgtatgagca cgacgccgaa ggggatcgac tcccgcgtgc
      601 agcggctcac catgtccagg tagcgggttt cgaatatctg caaaggcaac cggccgccgt
      661 cgaaaagaac gacgcgcagg gggaacagcg ggatctccat cggagaacct tagcacgctg
      721 ctgcatcgca gtcgcgctgc ggtcgcgaaa gggggtgctt gccgttagac tgcgcagccg
      781 tggactggct gcagatcatc atccttgccc tggtgcaggg catcacggag ttcctgccga
      841 tctccagctc ggctcatctc atcctcccgg cattgctcac cgagtggccc gatcaggggc
      901 tggcgttcga catagccgtg catgcgggca cgctgctggc cgtgctgtct tactttcgcc
      961 gcgagatcgg ccgcttcgct gccagtggtg cccgtttgct ggcccgcggg cagcgggatg
     1021 acgacgccga cctgcttctc aagctgattg ccgcgacgtt gcccatcgtt ctggtgggct
     1081 atctcgcgca cgactgggtg gagtccagcc tgcgcactgt gcccgtcatc gcttctgcca
     1141 ccatcgtctt tgcgcttgct ctgtggtggg cggacgggcg ccggagcggc gccgatcatc
     1201 cgtcgtggac ggcggcaatg ctcatcggcg ctgcgcaggt catcgccatc gtgcccggca
     1261 cctcccggtc gggtattacc atgacggccg cgctgctgct gggcttgagt cgtacgggtg
     1321 ccgcccggtt ctcctttctg ctctccatac ccacgattct gggcgcggcc gtgctggaag
     1381 cggcgtctct gggtgccggc gagcatgcgg ttcgatgggc ggagctggtc gccggcgcgg
     1441 tgcttgccgg ggcaggcgcc tactgctgca tacacttttt cgtgaagctg gtggagcgga
     1501 cggggatgct gccctacgtc gtctatcggc tgatgctggg caccttgctg ctcggtctcg
     1561 ccctgtgatc gtcgcgcgct gacctccggt atac
//
LOCUS       DBNHOX010000724         4352 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_122963, whole genome shotgun sequence.
ACCESSION   DBNHOX010000724 DBNHOX010000000
VERSION     DBNHOX010000724.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_122963"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="AC16UZ_15710"
     CDS             <1..445
                     /locus_tag="AC16UZ_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248183.1"
                     /translation="LGEIHRVLKPGGTLAVSVPRFGPEWLCWRLSDDYHQVEGGHVRI
                     FRSRELRRSIEACAPEPAGARPVAGKTQLSFQSRHFAHGLHSPYWWLRCLFWRRGESV
                     WPVRLYHRFLVWDLMRKPLLTRVLERALNPLIGKSVVMYFHRGAV"
     gene            442..1509
                     /locus_tag="AC16UZ_15715"
     CDS             442..1509
                     /locus_tag="AC16UZ_15715"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HIR6248184.1"
                     /translation="MTRPESLHRTARYIASVQQNDGAIPWFAGGITDPWDHVEAAMGL
                     SIGGHHAQAAAAYRWLARMQRADGSWLAAYSGARVADGTRAETNFVAYVATGTWHHYL
                     ISEDGKFLRSMWPTVKRALDFVTGLQAPTGEIYWAQDTRTGINKDALVTGCSSIYKSL
                     ECGIFMAAELNEDPGAWPACRARLGDAIILRPDRFDRTWESKSRYSMDWFYPVLAGLF
                     SQADARERLDERWETFVAPSLGCRCVSDQAWVTVAESCELAIACVASGEPDKAEELCD
                     DLARFQAEDGSWWTGYAFEDDALWPDERPTWTAGAVLLAADAMHGLTPASALFTDNAL
                     PDAPEHSERAGRFQRLEQARG"
     gene            complement(1441..2055)
                     /locus_tag="AC16UZ_15720"
     CDS             complement(1441..2055)
                     /locus_tag="AC16UZ_15720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6248185.1"
                     /translation="MKLPLDPDEIKGFLHPEEGARLYALASVASGLGPCLEVGSYCGK
                     STLYLGTACRDQGQVLYAVDHHRGSEEHQPGEAYHDPELFDAEIGSMESLMAFRRTMY
                     RAGLEGCVIPVVAASALAGRHWATPLALVFIDGGHSVEAAQTDYRTWAPHVVPGGFLA
                     IHDLFPDPLEGGQAPIDVYRLASASGLFEPLETTRTLGVLRRVG"
     gene            2178..2309
                     /locus_tag="AC16UZ_15725"
     CDS             2178..2309
                     /locus_tag="AC16UZ_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248186.1"
                     /translation="MHDENEREPVEELFAAEAPFAFPDQLYNEDDAEDNLSYVRGYN"
     gene            complement(2328..2516)
                     /locus_tag="AC16UZ_15730"
     CDS             complement(2328..2516)
                     /locus_tag="AC16UZ_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248187.1"
                     /translation="MAGVILTLILAFVAGGALWIGIGPRLKLAEDAERNGLLNLLAYV
                     AIALPVAFAVVFYLLELI"
     gene            complement(2556..3428)
                     /locus_tag="AC16UZ_15735"
     CDS             complement(2556..3428)
                     /locus_tag="AC16UZ_15735"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6248188.1"
                     /translation="MTYSFEGKVAIVTGAGGGLGRCHALELARRGAKVVVNDLGGAMD
                     GTGGSSAAADEVVAEIKAGGGEALANGGSVSDRQGAKSMVVDAMNAWGRVDVLINNAG
                     ILRDRSFGKMELDDFQIVVDVHLMGAVNVTREVWPIMKEQNYGRIVMTTSPTGLYGNF
                     GQTNYGAAKLGQVGFMNSLKIEGAKNNIYTNTIAPVAATRMTEGLIPEGSQVALGPEL
                     VTPAVIFLCSEEAPNGVIVQAQGGNYSVACIVENEGVNLGLDATAEDVGANFEKISDL
                     TGAKPRGMLQLSSM"
     gene            complement(3504..>4352)
                     /locus_tag="AC16UZ_15740"
     CDS             complement(3504..>4352)
                     /locus_tag="AC16UZ_15740"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR6248189.1"
                     /translation="EDEHDAFRQIRRFLSYLPGNVWQRAPRAPSDDDPTRMEQELLSA
                     VPRDSNAPFDMRVIVEMIVDLGSFFEIGATFGPSQICGLARLDGQPVGVLANDNRHYA
                     GAMTAQAAQKYRRFVEMCDTFHLPIVNFVDQPGFMIGPEAERAGTIRYGMAAVAAAVQ
                     ATVPWASIQVHKGFGVATAAHYGKGAYVLAWPSVETGALPLEGGVAVAFHREIAASDD
                     PDAKRAELESGMRAARSPFPRAESFAVHELIDPRETRPMLCEWVDWIQPRLDNLTGPV
                     RFGIRP"
BASE COUNT          787 a         1475 c         1346 g          744 t
ORIGIN      
        1 tctcggtgag atccatcgag tgctcaaacc cggcggaacg cttgcggtca gcgtgccccg
       61 attcggtccg gagtggctct gctggcgact ttccgacgac taccaccagg tggagggcgg
      121 ccacgttcgc atcttcaggt cccgggagct gagaaggtcc atcgaggcgt gcgccccaga
      181 accggccgga gcgcggccgg tggccggaaa gacgcaactg tcgttccaga gccggcattt
      241 cgcccacggc ctgcattccc cgtactggtg gctgcgctgc ctgttctggc gccggggcga
      301 gtcggtctgg cccgtccgcc tctatcaccg cttcctggtc tgggatctca tgcgcaaacc
      361 cctgctcacc cgtgtcctgg aacgcgccct gaacccgctg atcggcaaaa gcgtggtcat
      421 gtactttcac cggggtgccg tatgacgcgg ccggaatctc tgcaccgcac cgcccgatac
      481 atcgcctccg tccagcaaaa cgacggcgcg atcccctggt ttgcgggagg gatcacggat
      541 ccctgggacc atgtggaggc cgccatgggg ctgagcatcg gcgggcatca cgctcaggcc
      601 gcggccgcct accgctggct tgcccggatg cagcgggccg atggttcctg gctggctgcc
      661 tacagcggtg cgcgcgtggc ggacggcacc cgagcggaga ccaatttcgt cgcctacgtg
      721 gcaacaggca cctggcatca ttatctgatc tccgaggacg gcaagttcct gcgctccatg
      781 tggccgacag tgaagcgcgc gcttgatttc gtcaccggcc tgcaggctcc cacgggcgag
      841 atctactggg cccaggatac ccgcacgggc atcaacaagg acgccctggt caccggttgc
      901 agctccatct acaagagcct cgagtgcggc atcttcatgg ccgcggagct caatgaggac
      961 ccgggcgcct ggcccgcatg ccgggcgcga ctcggcgatg ccatcatcct gcgccccgat
     1021 cgcttcgacc gcacgtggga aagcaagtcc cgctactcca tggactggtt ctatccggtg
     1081 ctggcggggc tcttttcaca agccgacgca cgcgaacggc tggacgagcg atgggaaacc
     1141 ttcgtcgccc cttcgctggg ctgccgctgc gtctccgacc aggcctgggt cacggttgcc
     1201 gagtcctgcg agcttgccat cgcctgcgtc gcttccggcg agccggacaa agccgaggag
     1261 ctctgcgacg acctggcgag gttccaggcc gaggacggtt cttggtggac gggatacgcg
     1321 ttcgaggacg acgccctgtg gcccgacgag cggcccacct ggaccgccgg ggccgtgctg
     1381 ctggccgccg acgccatgca cgggctgacc ccggcatcgg ccctgttcac ggacaatgcg
     1441 ctacccgacg cgccggagca ctccgagcgt gcgggtcgtt tccagaggct cgaacaggcc
     1501 agaggctgac gccaggcggt agacgtctat aggcgcctgg ccgccttcga gaggatccgg
     1561 aaacagatcg tggatggcca ggaaaccgcc gggcacgacg tggggagccc aggtccggta
     1621 atcggtctga gcagcttcga cgctgtgccc accgtcaatg aacacgagcg caagcggcgt
     1681 cgcccagtgg cggcccgcca gcgcagacgc cgcaacaacc gggatcacgc atccttccag
     1741 acccgcgcgg tacatggttc tgcgaaacgc catcagcgat tccatgctgc cgatctcggc
     1801 atcgaagagt tccggatcat gatacgcctc acccggctgg tgctcctccg atccccgatg
     1861 gtgatcgacc gcgtagagca cctggccctg gtcccggcag gccgtaccga gataaagcgt
     1921 ggacttgccg cagtagctgc ccacctcgag gcagggccct aggcccgacg caacgctggc
     1981 gagcgcgtag agccgtgcgc cttcctccgg gtgaaggaac cctttgatct cgtcgggatc
     2041 gagtggaagc ttcatgtcga aaaaccgagc ttttcgtcgc aattcagcgc aaatccgacg
     2101 gcaacctcgc agaacgggat tgcaatttcg acatctgaca ctagactccc ctcacacgac
     2161 cctgccggtt caacaagatg cacgacgaaa acgagcgaga gcccgtcgag gagctgttcg
     2221 cggccgaggc accctttgcc ttcccggacc agctctacaa cgaggacgac gcagaggaca
     2281 atctcagcta cgtccgcggc tacaactgat tcagccctcg cgccagctca gatcagctcc
     2341 agcaggtaga acaccaccgc gaacgccacc ggcaaagcga tggcgacgta tgcgagcagg
     2401 ttgagcagcc cgttgcgctc ggcgtcctcg gcgagcttca gcctcgggcc gatgccaatc
     2461 catagcgcgc cgccggcaac gaatgccaga atgagcgtga gtattacccc tgccatatgt
     2521 gcccgtcggc gcgctgcaaa gatcctgccg ccagcctaca tgctgctgag ctgcagcatg
     2581 ccccgtggct ttgccccggt gagatctgag atcttctcga agtttgctcc cacatcctct
     2641 gcagtggcgt ccaatcccag gttgacgcct tcgttctcga cgatgcaggc caccgagtag
     2701 ttcccgccct gagcctgaac gatcacgccg ttgggcgctt cttcgctgca aaggaagata
     2761 acggccgggg tcacgagctc cggaccgagc gcaacctgcg agccctcggg aatgagtcct
     2821 tccgtcatcc gcgtggccgc caccggcgcg atggtgttgg tgtaaatgtt gttcttggcg
     2881 ccctcgatct tcagcgagtt catgaaaccc acctgaccaa gcttggctgc gccgtagttg
     2941 gtctgcccga agtttccgta aagacccgtg ggcgaggttg tcatgacgat tcgcccgtag
     3001 ttctgttcct tcatgatcgg ccacacttcg cgggtgacgt tgacggcacc catcaggtgc
     3061 acgtcgacga caatctggaa gtcgtccagc tccatcttgc cgaaggagcg gtcgcgcaga
     3121 atgcccgcgt tgttgatcag aacgtccacg cggccccatg cgttcatggc atcgacgacc
     3181 atgctcttcg caccctgccg gtccgatacg gacccgccat tggcaagggc ctcgccgccc
     3241 ccggccttga tctcggcgac gacctcgtct gccgccgcag acgacccccc ggttccgtcc
     3301 atggcgccac cgaggtcgtt gaccacgacc ttcgcccctc gccgggccag ctcgagggcg
     3361 tggcatcggc caagaccgcc gccggctccg gtcacgatgg caaccttacc ttcaaacgaa
     3421 taagtcatat tcttctccag ggctgtagtc agctaagcaa acgagagagc cgcgcattct
     3481 aatgcaatct gggcgccggg tgatcagggc cggataccga aacgaacggg gccggtgagg
     3541 ttgtccagac gcggctggat ccaatcgacc cactcgcaca gcatcggacg cgtctcccgg
     3601 ggatcgatga gctcgtgcac ggcaaacgac tccgcccggg gaaagggcga acgggcagca
     3661 cgcattccgg actcgagctc cgcccgcttc gcgtccgggt cgtcggaagc ggcgatctcc
     3721 cggtggaagg caacggcaac gcccccttcg agcggcaaag cgccggtttc caccgacggc
     3781 caggccaaaa cgtaagcacc cttgccgtaa tgggcggccg tggccactcc gaaacccttg
     3841 tgcacctgaa tcgacgccca gggcacggtg gcctgaacgg cggcggccac cgccgccatg
     3901 ccgtaacgga tcgtgcccgc tcgctcggcc tcaggtccga tcatgaagcc cggctggtcc
     3961 acgaagttca cgatgggcag atgaaaggtg tcgcacatct cgacaaagcg acgatacttc
     4021 tgcgccgcct gggccgtcat cgcgccggca tagtgccgat tgtcgttggc cagcaccccc
     4081 acgggttggc cgtccagcct ggccagcccg cagatctgac ttggcccgaa ggtcgccccg
     4141 atctcgaaaa aactgcccag gtccacgatc atctcgacga tgacccgcat gtcgaagggc
     4201 gcgttggaat cccgcggcac ggcggaaagc agctcctgct ccatgcgggt cggatcgtcg
     4261 tcgctcggcg cgcgcggagc gcgctgccag acattaccgg gcaggtaact cagaaagcgg
     4321 cggatctggc gaaaggcgtc gtgctcgtcc tc
//
LOCUS       DBNHOX010000725         1707 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_444141, whole genome shotgun sequence.
ACCESSION   DBNHOX010000725 DBNHOX010000000
VERSION     DBNHOX010000725.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_444141"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="AC16UZ_15745"
     CDS             <1..1278
                     /locus_tag="AC16UZ_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008030174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR6248190.1"
                     /translation="ITGQSGLRTGLTKVGLPGAELGIQDRDVTLAEVLRDMGYATGQF
                     GKNHLGDKDEHLPTNHGFDEFLGNLYHLNAEEEPEDPDYPKVPGFRERFGPRGVIHSF
                     ADGKVEDTGPLTRTRRETADEEFVAAAKDFVDRSVKADKPFFVWLNTTGMHFRTHPAE
                     KHLGKSGQGPYNDVMMAHDELVGQMLNQLDALGIAENTIVFYSTDNGVHFNTWPDAGV
                     TPFRSEKNTNWEGAYRVPAMVRWPGKIAPGQVSNEIMSHLDWMPTLVAAAGDVGLKDK
                     LLEGTRIGRKQSKLHLDGYNFLPYLTGQAENGPRREFHYLNDEGMPVGVRVGDWKIVF
                     AENRGKTMALWAEPFVTLRMFKIYNLRRDPFERAEHNSNSYWDWMIDTAPQGYLGLAA
                     TAQFLQSFQAYPPSQRPDSWSIDRLTEALLEQQ"
     gene            1275..>1707
                     /locus_tag="AC16UZ_15750"
     CDS             1275..>1707
                     /locus_tag="AC16UZ_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248191.1"
                     /translation="MNVRSAIAGGLLCICWISGAAWGEAEPIAQADLPPDAAPADPYG
                     RDTPRGSMEGFLESAGEGDFERAARYLDLRNLPNALNRYSPETLARGLNLVLERGTWL
                     DLTRLSNSPEGDLGDGLPTYRDPLDTLSTSRGDVELWLQHVP"
BASE COUNT          329 a          562 c          554 g          262 t
ORIGIN      
        1 atcactggcc agtcgggcct gcgaacgggg ctgaccaagg tgggcctgcc cggcgccgag
       61 ctgggcattc aggaccgaga cgtgacgctg gccgaggtgc tcagggacat gggctacgcc
      121 accgggcagt tcggcaagaa ccacctgggc gacaaagatg agcatctgcc caccaatcac
      181 ggctttgacg agttcctggg caacctctat cacctgaacg ccgaggagga gcctgaggat
      241 cccgactatc caaaggtccc cggcttccgg gaacggttcg ggccgcgcgg cgtgatccac
      301 tcgttcgccg atgggaaggt agaggatacc gggccgctca cgcgcacgcg cagggaaacg
      361 gcggacgagg aattcgttgc cgccgcgaag gatttcgtcg accggtccgt caaggccgac
      421 aagccgttct tcgtctggct caacaccacg ggcatgcact tccgcaccca cccggcggag
      481 aagcacctgg gcaagtcggg ccagggcccg tacaacgacg tcatgatggc ccacgacgag
      541 cttgtcgggc agatgctgaa ccagctggac gcgctgggca ttgccgagaa caccatcgtc
      601 ttctactcca cggacaacgg cgtgcatttc aacacctggc cggacgccgg ggtcaccccg
      661 ttccgaagcg aaaagaacac caactgggaa ggcgcctacc gcgtgccggc catggtgcgc
      721 tggcccggca agatcgctcc cggtcaggtc tccaacgaga tcatgtccca cctggattgg
      781 atgccgacgc tcgtggcagc agccggggat gtcgggctca aggacaagct tctcgagggc
      841 acgcggatcg gccgtaagca gtcgaagctg catctggacg gctataactt tctgccctat
      901 ctgaccggcc aggccgaaaa cgggccgcgc cgggagttcc actacttaaa cgacgagggc
      961 atgccggtgg gcgtgcgggt cggggactgg aagatcgtct tcgccgagaa ccgcggcaag
     1021 acgatggcgc tgtgggcaga gccgttcgtc acgctcagaa tgttcaagat ctacaacctg
     1081 cgccgtgatc ccttcgagcg tgccgagcac aactcgaact cctactggga ctggatgatc
     1141 gacacggcgc ctcagggcta cctgggcctc gccgccacgg cgcagttcct gcagtccttt
     1201 caggcgtacc cgccgagcca gcggccggac agctggtcca tcgaccggct gaccgaagcg
     1261 cttctcgagc agcaatgaac gtccgcagcg ccatcgccgg gggcctcctg tgcatctgct
     1321 ggatcagcgg cgcggcctgg ggagaagcgg aaccgatcgc ccaggccgat ctgcccccgg
     1381 acgccgcccc tgccgacccg tacgggcgcg acaccccgag gggatcgatg gaaggcttcc
     1441 tggaatccgc cggcgagggc gacttcgagc gtgcggccag atatctggat ctgcgaaacc
     1501 tgccgaatgc tctgaatcgg tactcaccgg aaacgctcgc ccgagggctc aatctggtgc
     1561 tggagcgggg aacctggctg gacctgaccc ggctcagcaa cagcccggaa ggcgacctcg
     1621 gcgacggcct gccaacctat cgggatcccc tggacaccct gtcgaccagt cgcggcgacg
     1681 tcgagctttg gctgcagcac gtcccgt
//
LOCUS       DBNHOX010000726         2319 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1279828, whole genome shotgun sequence.
ACCESSION   DBNHOX010000726 DBNHOX010000000
VERSION     DBNHOX010000726.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1279828"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="AC16UZ_15755"
     CDS             <1..433
                     /locus_tag="AC16UZ_15755"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6248192.1"
                     /translation="DLATYNQALSPATERADPENLRWALTRLRRLAHTGYRIFIISDF
                     EPMGDHWRDALKGLSRHNEVVAVRIFDPLERDLPPPGRYTVTDTEQRWQFHSGNRLLR
                     KTYRERFADHEIELKTICEGSGVRYRSLATDARVGPMAEWA"
     gene            433..909
                     /locus_tag="AC16UZ_15760"
     CDS             433..909
                     /locus_tag="AC16UZ_15760"
                     /inference="COORDINATES: protein motif:HMM:NF025674.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HIR6248193.1"
                     /translation="MQPNPLEQLRDIHLPPDPGWWPPALGWWLLALVIAFVCAAGIRA
                     IYEYRRRRRPVRQARRLYERLSEHLRSGSLSGEAFTHECNELLKRLLVHGFGERDARG
                     ATDETWLAMLDERYGERAFTTGPGRLLGNERFRPDPEFATDELDALMQRFLKRVQP"
     gene            906..1907
                     /locus_tag="AC16UZ_15765"
     CDS             906..1907
                     /locus_tag="AC16UZ_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012075094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6248194.1"
                     /translation="MIEWLWPWAFLLAPLPLAMRLLVSPVARQQAALTVPSVAAFRAT
                     DDSAGTASGRFPWRAVALWLIWLALVTATARPQWTGDPVTLPVTGRDLMLAVDISGSM
                     GTEDLELGGRMVNRLTVVKNVVSQFVDARRGDRVGLILFGTNAYLQAPLTFDLDSVNR
                     LLVEAPVGIAGGKTAIGDAIGLAVKRLRRRPEGERVLILLTDGASNVGEVAPIKAAEL
                     ARQEEIRIHTIGVGADEMRMPSLFGVFGSRVINPSAELDEETLQTIADATGGRYFRAQ
                     NTQRLQEIYTIIDALEPVEQDAETYRPIAALYYWPLGAAWFMSMLLIAGNWRGWGRD"
     gene            1900..>2319
                     /locus_tag="AC16UZ_15770"
     CDS             1900..>2319
                     /locus_tag="AC16UZ_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248195.1"
                     /translation="MIESLQFIRPWWLLGLIPAIGLAALWARNRAAGSHWESIIAPEL
                     LNVLLEPTGKGGMRRTAVLAALALAAATVGLAGPTWERLPQPVEQRSDALVVVLDLSL
                     SMYAQDVPPSRIVRARQKITDVLRARDEGFTGLVAYAG"
BASE COUNT          371 a          777 c          780 g          391 t
ORIGIN      
        1 cgatctggcc acctataatc aggcgctctc ccccgcgacg gagcgcgcag accctgaaaa
       61 tctgcgctgg gcgctgacgc gcctgcggcg cctggcccac acgggttatc ggatattcat
      121 catcagcgac ttcgaaccca tgggcgatca ctggcgcgat gcgctcaagg ggctgtcgcg
      181 ccacaacgag gtggtcgccg tccggatatt cgacccgctg gagcgagacc tgccgccacc
      241 aggccgctat acggtgaccg ataccgagca gcgctggcag tttcacagcg gcaaccggct
      301 gcttcgcaag acctatcggg aacgcttcgc ggatcacgag atcgaactga agaccatctg
      361 cgagggctcc ggcgtccgct accgttcgct ggcgaccgac gcgcgggtgg ggccaatggc
      421 cgagtgggcg tgatgcagcc gaatcccctg gagcagctca gagacatcca tctgcccccg
      481 gaccccgggt ggtggccgcc ggccctcggt tggtggctgc tggcattggt gatcgctttt
      541 gtctgcgcgg ccggcatccg ggccatctac gagtatcggc gccggcgccg tcccgtccgg
      601 caggcccggc gtctctacga gcgtctcagc gagcacctca ggtcggggtc cctttccggc
      661 gaggccttca cccacgagtg caacgagctt ttgaagcggt tgctcgttca cggcttcggc
      721 gagcgtgacg ccagaggggc cacggacgaa acctggctgg cgatgctgga cgaacgttac
      781 ggcgaacggg cgttcaccac cggtccgggc cggcttctcg gcaacgagcg ctttcgcccc
      841 gacccggagt ttgccaccga cgaactcgac gcgctgatgc agcgcttcct gaagcgggtg
      901 cagccatgat cgagtggctg tggccgtggg ccttcctctt ggcgccactg cccctggcaa
      961 tgcggctgct cgtcagcccc gtggcgcgtc agcaggcagc tctaacggtg ccaagcgtcg
     1021 cagctttccg cgccacggac gatagcgccg gcacggccag cggacgcttt ccctggcgcg
     1081 cggtcgcgct gtggctgatc tggttggcgc tggtcacggc caccgcaagg ccgcaatgga
     1141 cgggcgatcc ggtcacgctt ccggtaaccg gaagggacct catgctggcc gtggacatat
     1201 ccggaagcat gggcaccgag gatctggagc tgggcgggcg aatggtcaat cgcctgacgg
     1261 tggtcaagaa cgtcgtcagc cagttcgtgg acgcccggcg cggtgaccgc gtcgggctga
     1321 tcctgttcgg caccaatgcc tacctccagg cgccgctcac gttcgacctg gacagcgtca
     1381 accgcctgct ggtggaagct cccgtgggca tcgccggcgg caagacggcg atcggtgacg
     1441 ccatcggtct ggccgtcaaa cggctgcgca gaaggcccga gggcgaacgc gtgctcatcc
     1501 ttcttaccga tggggcgagc aacgtcggcg aggtcgcgcc gataaaggcg gcagagctcg
     1561 ctcgccagga ggagatccgc atccacacca tcggcgtggg cgcggacgag atgcggatgc
     1621 cgagcctttt cggcgtcttt gggtccaggg tgatcaaccc ctcggcggag ctcgacgagg
     1681 aaaccctgca gaccatcgcc gacgccaccg gagggcgcta cttccgcgcc cagaacaccc
     1741 agcgccttca ggaaatctac accatcatcg acgcgctcga gcccgtggag caggatgccg
     1801 agacctaccg gcccatcgcc gcgctctact actggccgct gggcgccgcc tggttcatgt
     1861 cgatgctgct gatcgccggc aactggcgag gctggggccg tgattgagtc attgcaattc
     1921 atccgcccgt ggtggctgct tggcctgatt cccgccattg gcctggctgc gctgtgggcc
     1981 cgaaaccgcg ccgcggggtc gcactgggag tccatcatcg ccccggagct tctcaacgtt
     2041 ctgctcgagc caaccggcaa ggggggcatg cggcgaacgg ccgtcctcgc ggcattggcg
     2101 ctggcggcag caaccgtcgg tctggcgggg ccgacgtggg aacgcctgcc tcaacctgtg
     2161 gagcagagaa gcgacgctct ggtggtcgtg ctcgatctct cgctgtccat gtacgcgcag
     2221 gatgtgccgc cctcgcgcat cgttcgcgct cgccagaaga tcacagacgt ccttcgggcc
     2281 cgggacgagg gtttcaccgg cctggtcgcc tacgcggga
//
LOCUS       DBNHOX010000727         2068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1538500, whole genome shotgun sequence.
ACCESSION   DBNHOX010000727 DBNHOX010000000
VERSION     DBNHOX010000727.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1538500"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="AC16UZ_15775"
     CDS             <1..902
                     /locus_tag="AC16UZ_15775"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6248196.1"
                     /translation="GQVLLATEHLSIDAFIRTMLDSQEGLHGTVAIDTPVMALGVARV
                     VDSRSPDLNQGDAVFGPMGAQTHLVAPGGMYRKLEEGDVPPRSYLGALGMTTGLTAYA
                     GMLKVGQVAEGDTVVVSAAAGAVGTIACQLAKIAGAKVIGIAGGPAKVAYLIDEIGCD
                     GAIDYKNDDVAEKLRALAPAGVNVFFDNVGGELLDIVLDQIAQGARVVICGAISQYNH
                     MEEVRGPRLYLRIPERNASMLGFTVDRFAADFPEMEADISRWMAEGKLKMPEHVVEGI
                     EQFPKALTMLFTGGHMGKLLVAP"
     gene            971..>2068
                     /locus_tag="AC16UZ_15780"
     CDS             971..>2068
                     /locus_tag="AC16UZ_15780"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6248197.1"
                     /translation="MRGIFLALVAVCLQVPVLAVAAEIDFTRHLVRVESAAGQHPGPD
                     VGPARPFFGVAGPARVIVTARSGEPVVTVRLNGVDVLDGQALRQTTSLEIPVELAEAN
                     TIAVGLDGRDGAAASVRVKQRADVALNVISWVHFNTNVSDFNEARAFYGVLGFETMSG
                     FPDTNTLAMAHALGIQTPTAYDGSQGEEAGGYLLHGELVGPDGFWGGVIDLIEFTIPR
                     NEEPPYPGLNHLGMARALMHTGDIAAAYDQLVGAGVNFLSPPATRSDGTRFAVFTDPD
                     GTFYELVELEGPGGEASEKPVIGVGRVNVNVSDFERSEAWYRMFGYVATRKLPPTDSA
                     EVAAAMGFDAPYRIDGAVLSHEVDGSQIELVQ"
BASE COUNT          351 a          669 c          705 g          343 t
ORIGIN      
        1 aagggcaggt gctgctggcg acggagcatc tctcaatcga cgccttcatc agaacgatgc
       61 tggactccca ggaagggctc catggcacgg tggccatcga tacgcccgtg atggccctgg
      121 gcgtggcgcg cgtcgtggac agccgctcgc ccgacctgaa ccagggtgat gccgtgttcg
      181 gccctatggg ggcacagact cacctcgtcg cgccgggcgg catgtaccgg aagctcgagg
      241 agggcgacgt gccgccgcgc agctatctcg gcgcgctggg gatgaccacg ggccttacgg
      301 cctatgccgg catgctcaag gtgggacagg tggccgaagg cgacaccgtg gtggtttccg
      361 ccgccgcggg tgcggtggga accatcgcgt gccagctggc caagatagcc ggcgccaaag
      421 tcatcggcat cgccggcggg ccggccaagg ttgcctacct gatcgacgag atcggttgcg
      481 atggtgccat cgactacaag aacgacgacg tggcagagaa gctaagagcc cttgcgcctg
      541 ccggcgtgaa cgtgttcttc gacaacgtgg gcggcgagct gctcgacatc gtgctcgatc
      601 agattgccca gggagccagg gtggtgatct gcggcgccat ctctcagtac aaccacatgg
      661 aagaggtcag ggggccgcgc ctttatctca ggatacccga gcgcaatgcc agcatgctgg
      721 gcttcaccgt ggaccgcttt gcggcggact ttccggagat ggaggccgac atctcgcgct
      781 ggatggccga aggaaagctc aagatgcccg agcacgtcgt cgagggcatc gagcagttcc
      841 ccaaagcgct gaccatgctg ttcacgggcg gccacatggg taagctcctg gtcgctccgt
      901 gatccggcac gacgccgcac gcgcgcggcg gcttttgccg gaccatcgct ccggggcaaa
      961 gccgcggcgc gtgcgcggga tattcctcgc gctggttgcc gtttgcctgc aggtgcccgt
     1021 cttggcggtc gccgctgaga tcgacttcac ccgccatctg gtacgggtcg aaagcgcggc
     1081 cgggcagcac cccgggcccg atgtcgggcc ggcgcggccg ttcttcgggg ttgccggccc
     1141 tgcccgggtt atcgtaaccg cacgatccgg tgagccggtg gtgaccgtcc ggctgaacgg
     1201 cgtcgacgtg ctcgacgggc aggcgttacg gcaaacgacc tccctggaga tcccggtaga
     1261 gcttgccgag gcgaacacca ttgccgttgg cctcgacggc cgggacggcg ccgcggcgtc
     1321 ggtgcgcgtc aagcagcgcg cggacgtcgc gctgaacgtc atctcctggg tgcacttcaa
     1381 caccaacgtg agcgatttca acgaagcccg cgcgttttac ggcgtgctcg ggttcgaaac
     1441 catgagcggc tttccggaca ccaacaccct ggcgatggcc cacgccctgg gcatccagac
     1501 gccgacggcc tacgacggct cccagggaga agaggccggc ggctacctgc tccacggcga
     1561 gctggtgggc cccgatggtt tctggggcgg cgtcatcgat ctcatcgagt tcaccattcc
     1621 gagaaacgaa gagccgccgt acccgggcct taaccacctg ggcatggcgc gggctctcat
     1681 gcacacgggc gacattgcgg ccgcctacga ccagctcgtg ggagccggcg taaactttct
     1741 ctctccgcct gccacccgct cggacggtac ccggtttgcc gtcttcaccg atccggacgg
     1801 caccttctac gagctggtag agctggaagg cccaggcggc gaggcatccg agaaacccgt
     1861 catcggtgtt ggtcgggtaa acgtcaacgt gagcgacttc gaacgctccg aagcctggta
     1921 ccggatgttc ggatacgtag ccacccgcaa gctgcccccg acggactccg ccgaggtggc
     1981 agcggccatg gggttcgatg cgccctacag gatcgacggg gccgtgctca gccacgaggt
     2041 ggacggctcg cagatcgagc tggtccag
//
LOCUS       DBNHOX010000728         2628 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_444795, whole genome shotgun sequence.
ACCESSION   DBNHOX010000728 DBNHOX010000000
VERSION     DBNHOX010000728.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_444795"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="AC16UZ_15785"
     CDS             <1..298
                     /locus_tag="AC16UZ_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HIR6248198.1"
                     /translation="WLAWAQDQDPDLESAWLVLARLGELATNPNGFEPLDIDEQLEDL
                     APDLIPSHVEILHQARLAQAKPPLDPANENRPKVGRNDPCPCGSGKKFKKCCLH"
     gene            953..2452
                     /gene="ltrA"
                     /locus_tag="AC16UZ_15790"
     CDS             953..2452
                     /gene="ltrA"
                     /locus_tag="AC16UZ_15790"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HIR6248199.1"
                     /translation="MSMYADEKSDEGVVPEKRPNKWGMPPAEAVEGRTSPKGNGGRTT
                     AVRTQSRAAASNGLAAVRQAARQSREVRFTALLHHITIELLEQSYFALKRNSAPGIDG
                     VTWQAYGEDLEEKLTDLHARIHRGGYRARPARRTDIPKPDGSQRPLSILCLEDKIVQQ
                     AVVFVLEAIYEADFMGFSYGFRPGRGQHDALDALHAGIYRRQVNWVLDADIQGFFDAM
                     SHDWILRFLRHRIADKRILRLIAKWLKVGITEDGQVTRSSRGTPQGAVISPILANIYL
                     HYVFDLWSDAWRRKKASGDVIVIRYADDIVLGFQHEREAQAFLHDLQARMRAFELALH
                     PDKTRLIRFGRHAAEQRRRLGQGKPETFDFLGFTHFCTRSRKYGSFVIGRKTIKKRMR
                     AKLQVIKMELRRRLHHSIAETGAWLRRVLTGHLNYYAVSGNDPSLWWFFTQVRWHWLR
                     TLRRRSQRAFMSWETFTRRTNRFFPPIRILHPLPCHRFDARTRGRSPVR"
BASE COUNT          539 a          806 c          841 g          442 t
ORIGIN      
        1 gtggctcgcg tgggcccagg accaagaccc cgatctcgag agcgcctggc ttgtgctcgc
       61 tcgtctcggc gaactggcaa ccaatccgaa tggcttcgag ccgctggata tcgacgagca
      121 attggaagac ctcgcccccg acctcatacc ctcacatgtg gagatactac accaggcccg
      181 tctcgcgcag gcaaagccgc ccctggatcc cgccaatgaa aaccgcccaa aagttgggcg
      241 caacgatcca tgcccgtgtg gctccgggaa aaagttcaaa aagtgctgcc tgcactgacc
      301 gcgcgggatg atcggccacg gctgttttgc ctgtactccc ggagcaaagc cccttctccc
      361 aaatagaata tataggcgcg ctggggtctt gcccttggtg tgagccgcat gttcgccgac
      421 aggtatctgg tcagatttgc caggcgtagc tgcttccgcc gctggtgcat ccggttgcgc
      481 ggttgcagag gaatggggcc tgcctcgggg gtcgatttcc gccgtgtccg cttcgcactc
      541 agcagccgaa acaccttggc gcgcctagaa acgtcgcgaa tgacccggag cggcgtgcgc
      601 caggacaagc tggcaaagga taggagatga aagttctcta cgaagtaagg tctagccaac
      661 cacttcggcc ccgagtgatg cgcggttgtc cgcgagggca gacgtgaagc gttcacaggg
      721 gagaccatgg gctgggtatt gagctgcgaa acgggtcgaa ttccgaggtg ttgatgccgt
      781 tctgctggcc agaagacaac atcatctccc gcgctatccg gcgagcgggc gatggacctc
      841 gcgcagtcgg agaccccatg cacgtggaga cgtcctttgt acggaacctg ggagatctca
      901 tccgtgcccg gtgtggcatg ccgggccggc tcatgaaggc gagagctgca cgatgagcat
      961 gtacgcggat gagaagtcgg acgagggagt agtacccgag aagcggccga acaaatgggg
     1021 catgcccccg gcggaggccg tggagggaag gacctcgccc aaggggaacg gcggtcggac
     1081 gaccgcggtc cggacacaga gccgggcagc cgcgtcgaac gggctggccg ccgtgcgcca
     1141 agcggcgcga cagagtaggg aagtacggtt cactgccctg ctgcatcaca tcaccatcga
     1201 acttctggag cagagctact tcgcgttgaa gcggaactcg gctcccggga tcgacggcgt
     1261 gacgtggcag gcctatgggg aagacctcga agagaaactt acggacctgc acgctaggat
     1321 acaccggggc ggctaccgcg cccgaccggc acgacggact gacatcccga agccggacgg
     1381 ctcgcaacgg ccgctcagca tcctgtgtct cgaggacaag atcgtccaac aggcggtggt
     1441 gttcgttttg gaagcgatct acgaggcgga cttcatgggg ttctcctacg gctttcggcc
     1501 ggggcgcggc cagcacgatg cgctggatgc cctccacgcc gggatctacc ggagacaggt
     1561 gaactgggtg ctggacgcgg atatccaagg cttcttcgat gccatgagcc acgactggat
     1621 cctccgtttc ctccggcacc gtatcgcgga caagcgcatc ctgcgcctca tcgcgaagtg
     1681 gctgaaggtc ggcatcacgg aggacggcca ggtcacacgt agcagcaggg gaacgccaca
     1741 gggagcggtg atctcaccga tcctggcgaa catctacctg cactacgtgt tcgacctgtg
     1801 gtccgatgcc tggcgccgta agaaggcctc gggcgacgtg atcgtcatcc gctacgcgga
     1861 tgatatcgtg ctcggcttcc agcatgagcg tgaggcgcaa gctttcctgc acgatctgca
     1921 ggcaaggatg cgcgcgttcg agttggcgtt gcaccccgac aagacccggc tgatccggtt
     1981 cggtcgccat gcggccgagc aacgcagaag gctggggcag gggaagccgg agaccttcga
     2041 cttccttggc ttcacgcatt tctgcacgcg atcacgcaag tacgggtcgt tcgtcatcgg
     2101 gcgcaagacg atcaagaaac ggatgcgggc aaagctacag gtgatcaaga tggaattacg
     2161 cagacgactg caccattcca tcgcggagac cggggcatgg ctccgacggg tgctcaccgg
     2221 gcacctgaac tactacgccg tctccggcaa cgatccgagc ctgtggtggt tcttcaccca
     2281 ggtacgctgg cactggctcc ggacactccg gcggcgcagc cagcgagcgt ttatgtcgtg
     2341 ggagacgttc acccgacgca ccaaccgttt ctttccgccg atccggatct tgcatccctt
     2401 accctgtcac cgcttcgacg ccagaacccg agggaggagc ccggtgcgtt agcagcgcac
     2461 gccgggatct gcgcgggggg agcggggcaa cccgcttccc taccgcgacc gaacgacaaa
     2521 agcgccagcg ccacccccac atgaacgccg atatagacca ggaacagcac gaaaaagatc
     2581 gaaacgattc cgacctccac ggcgctgagc ccgaaaacgt tgtcgagg
//
LOCUS       DBNHOX010000729         7893 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_638574, whole genome shotgun sequence.
ACCESSION   DBNHOX010000729 DBNHOX010000000
VERSION     DBNHOX010000729.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7893)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7893
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_638574"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="AC16UZ_15795"
     CDS             <1..461
                     /locus_tag="AC16UZ_15795"
                     /inference="COORDINATES: protein motif:HMM:NF023167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="intermembrane phospholipid transport protein
                     YdbH family protein"
                     /protein_id="HIR6248200.1"
                     /translation="AAFLVIATGIELADVLALEGESVTGSGILDGELPVELLEGGTRI
                     TNGRFLARAPGGVLRYPDAGKAAEALGQQVGGFALAPLVDFRFEVLDAHVDLQPSGDL
                     LLGVRLEGRSPEFERPYHYNLNISENIPALLKSLRISEEFQRRLEEVLQR"
     gene            606..788
                     /locus_tag="AC16UZ_15800"
     CDS             606..788
                     /locus_tag="AC16UZ_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HIR6248201.1"
                     /translation="MLLALALALAVLAAAGCTPTVKVATDEPITINLNVNIKHEILVR
                     VDKELNDLFGEDSDLF"
     gene            834..1136
                     /locus_tag="AC16UZ_15805"
     CDS             834..1136
                     /locus_tag="AC16UZ_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family probable chaperone protein"
                     /protein_id="HIR6248202.1"
                     /translation="MLALVFSAVAVAAMGLDAAKAQGLVGEQHDGYLGIVSSATPEVR
                     ALVSDVNAKRRAEYQRIAQTNGIDVKDVELLAGQKAIQRTAPGHYVKLEGQSWRPK"
     gene            1190..2380
                     /locus_tag="AC16UZ_15810"
     CDS             1190..2380
                     /locus_tag="AC16UZ_15810"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR6248203.1"
                     /translation="MQTFVTHLECSLTGEHYPADQVHGLSAAGRPLLVRYDLTGLSRA
                     VGKADIAGRQGGFWRYREFLPVRRTENVVSLGEVVTPMIPIAGPAGGSVWVKDEGRLP
                     TGSFKARGLALAVAMARELGLSHLAMPTNGNAGAALAAYAARAGLRATVLCPSDTPEI
                     NVREIAMQGAEVWRVDGLINDCGRIVGEAAGRTGWFDLSTLKEPYRIEGKKTMGIELA
                     EQLGWRLPKAIFYPTGGGTGLIGMWKAFAELEAIGWIGPERPQMFAVQAEGCAPIVRA
                     FERGDEHAELWENAHTAAAGIRVPAAVGDFLILRAVRESGGAALAVSDADLLAAQRRI
                     AAQEGLLLCPEGAATYVAWRQALEAGLVGAEDEVVLFNCATGLKYPMPDESRTFTRLD
                     DLIG"
     gene            complement(2406..3233)
                     /locus_tag="AC16UZ_15815"
     CDS             complement(2406..3233)
                     /locus_tag="AC16UZ_15815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6248204.1"
                     /translation="MPKDNADQIEFWNGPTASNWVAAEERMDELLAPITTAILQAAAV
                     SPGEAVLDVGCGCGATSRAMAAVGARVTGVDVSAPMLARARQRADDESLSFIEADAQS
                     HPFDPGFDLLFSRFGVMFFADPVAAFANLRGALNPGGRLCFICWRAPGENPWLSIGMA
                     AAQPFLPPAETPDPRAPGPFAFADPDYLASVLADAGFEDVAITPHNPEMRLGADLTEA
                     MEFVTGVGPLSNVLKELAADLREAALAAVREALAPFAGPSGVLLGGACWIAQGRRGA"
     gene            3425..4099
                     /locus_tag="AC16UZ_15820"
     CDS             3425..4099
                     /locus_tag="AC16UZ_15820"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR6248205.1"
                     /translation="MAGGSTERGDERDALVALIESEVADTADYTGRSKLRPAVLEAMC
                     AVPREEFVPPEEAYAAYANVPLPIGHFQTISQPYVVALMTDLLDPEPGDVMLEVGTGS
                     GYQAAVLSTLVSRVYSLEVVPQLAASAAERLARLGYDNVVTREGDGNLGWPDHAPYDG
                     IVVTAGAPEVPPALIDQLKPGAALVIPVDAGFNQMLRRITRDPDGDVHTEDVLPVAFV
                     PLVKAT"
     gene            4170..5447
                     /locus_tag="AC16UZ_15825"
     CDS             4170..5447
                     /locus_tag="AC16UZ_15825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6248206.1"
                     /translation="MAGVWQELDRIVRPQGQVFYGWWIVLASSGVQWLAAVLWMQSYG
                     AYMVLLQQDFGWSKAMVAGAFALTRIESGILGPLQGWLVDRFGPRAILTIGTVLFGVG
                     FILFGQVESLLTFYLTFALIALGASLGGFATLMVAIVNWFSRHRSKAIAVSQLGFSLG
                     GLSVPLIIWALEAFGWRATAVVSGLLVLVVGLPLVQVVRHRPEAQGELADGHAASGGG
                     PEGRFSLGGDFTARQAMRTRSFWLISFGHALALLTVSAMMVHLVPHLTEGMGYSLSSA
                     GMVVAVLTAFQLLGQLSGGYLGDLFNKRLICGVCMFAHAGGLLLVTYADSIVGVLAFT
                     LLHGLAWGTRGPLMVSLRADYFGPTSFGTIMGFSSLIVMLGMSGGPIIAGIMADVSGD
                     YHSGFTLLASLSFMGSLCFFAATPPSPPARASA"
     gene            5510..6199
                     /locus_tag="AC16UZ_15830"
     CDS             5510..6199
                     /locus_tag="AC16UZ_15830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HIR6248207.1"
                     /translation="MPEFQGFPPRTLKFLKALERNNDRDWFRENKALYERDVLDPSLD
                     FIATMGPKLAAISDHFVAIPKRTGGSLMRVYRDTRFSKDKTPYKTNIGIQFRHELGKD
                     VHAPGYYLHIDTAGCFVAAGMWRPDAPSLARVRELIAERPGDWQKARDARPFKAAFEL
                     GGESLKRPPKGFAPDAPHLQDLRRKDFIASANVATGELGQPQLVDVVAELYARATPFM
                     RFLCRAIEVEF"
     gene            6382..7320
                     /locus_tag="AC16UZ_15835"
     CDS             6382..7320
                     /locus_tag="AC16UZ_15835"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6248208.1"
                     /translation="MNNTRLLVALAWLSLAPAGWAEDECTPSRWGSDDEIGNVNLITT
                     QSVLAASRLIKTGKTYSLGIVVDGNTPAFPPRSLSLQIVQPGQQEGNRPLSRMTYNDD
                     VFQGWFGIGSQLDGLGHAGDEGMYYNCNHARDFSDIAGLTKLGIEKVPPIVARGIVLD
                     MTAHFGVEHLAGGQTFSVDDVRAVEKKQGTPVREGDVVLFHTGWTDAMLETDPAGWSA
                     SAPGISKAVAGYLAGRNVVAVGSDTWGLDVLPPEREDEPFISHLILLQQNGIYILETM
                     NTGALVRDEAHEFLFVLGQAKVRGAVQMFINPIAIR"
     gene            7348..7845
                     /locus_tag="AC16UZ_15840"
     CDS             7348..7845
                     /locus_tag="AC16UZ_15840"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6248209.1"
                     /translation="MTVEVNGIAHIQLTVRDPARCVPFWERLCHFLEMSTLIRAEEIV
                     YCIGSRTGVLVRGAPEDKRDRAFDQDRPGLHHLCFRARSREDVDAIYEFVAGELDAHV
                     VHPPEDGSHFAPGYYSVLFEDPDGIRVEVNFVPGRGHFGSEGRLGPGGRGPASRYGEE
                     GLGDG"
BASE COUNT         1248 a         2538 c         2670 g         1437 t
ORIGIN      
        1 ccgccgcttt tctggtgatc gcaaccggga tcgagctagc cgacgtgctg gctctggaag
       61 gagagtcggt gaccggatcg ggcattctcg atggcgagct gcctgtcgag ctcctcgagg
      121 gcggtaccag gatcacgaac ggacgtttcc tcgctcgggc gcccgggggc gttctgcgct
      181 atccggatgc ggggaaggcg gccgaagcgc tcggccagca ggtcggcggg ttcgctctcg
      241 cgccgctggt ggatttccga ttcgaggtgc tcgatgcgca tgtcgatctc cagccgagcg
      301 gcgacctcct gctcggggtt cggttggaag ggcgcagtcc ggaattcgag cggccctatc
      361 actacaacct gaacatctcg gagaacattc cggccctgct caaatccctg cgcatttccg
      421 aagagttcca gcgacgcctg gaagaggttc tgcaaaggtg agcttgcggg gggtgtcggc
      481 gctgggtaat gttcagctcc ggttcacagt gcgccaggta ctctttgatg atgagtaact
      541 cgttcgaatt gccggtttcc tgccgccgga tcccaccggt caggccgcgt acggccgcct
      601 cagggatgct gctcgcgctc gcccttgctc tcgccgttct cgccgctgcg ggatgcaccc
      661 cgacggtgaa ggtcgcaacg gatgagccca tcaccatcaa ccttaacgtc aacatcaagc
      721 acgagattct cgtgagagtg gacaaagagc tgaatgacct gttcggcgaa gacagtgatc
      781 tcttttaagg cgacgcgatg aatatgaaac caatctttcg gcaaatcgtt ttcatgctcg
      841 cgctggtctt ctcggcagtg gccgttgcgg ccatgggcct ggatgcggca aaagcacagg
      901 gtctggtcgg agagcagcac gacggttacc tgggtatcgt ctcgtccgcc acgcctgaag
      961 tcagggccct cgtctccgat gtcaatgcca agcggcgcgc ggagtatcag cgcatcgctc
     1021 aaaccaacgg catcgacgtc aaggacgtgg agctgcttgc cggccagaag gccattcagc
     1081 gcaccgcccc cggtcactac gtcaagctcg aaggtcagag ctggcggccg aaatagccgg
     1141 cgagcctggc tgcgagagcg ggctcgccca accccagtcc taaaccgctt tgcagacctt
     1201 cgttacccat ctcgagtgct cgcttaccgg ggagcactac ccggccgatc aggtccacgg
     1261 cctctctgct gcggggcgac cgctgctcgt cagatacgac ctgacgggtc tttcgagagc
     1321 ggtgggcaag gcggacatcg ccggccgcca gggcggcttc tggcgttacc gggagtttct
     1381 gcccgtgcgt cgcacggaga acgtggtctc cctgggagaa gtcgtcaccc ccatgatccc
     1441 gattgccggc cctgccggcg gcagcgtctg ggtcaaggat gagggccgac tgcccaccgg
     1501 ttcgttcaag gcaagaggcc tcgctctggc cgtggccatg gccagggagc tgggtctttc
     1561 ccacctggcc atgcccacca acggtaacgc cggggcggca cttgcggcct atgcggcgag
     1621 ggccggcctc cgggccaccg tgctgtgtcc ctcggatacc ccggagatca acgtgcgcga
     1681 gatcgcgatg cagggcgcgg aggtatggcg cgtggacggg ctcatcaacg actgcgggcg
     1741 catcgtgggc gaggcggccg gacgtaccgg ttggttcgat ctttccaccc tgaaagagcc
     1801 ctaccggatc gagggcaaga agacgatggg catagagctc gccgagcagc tcggctggcg
     1861 cctgcccaag gccatcttct acccgaccgg cggcggaacc ggtctgatcg gcatgtggaa
     1921 agcgttcgcc gagctcgagg ctattggctg gatcgggccg gagcgaccgc agatgtttgc
     1981 cgtgcaggcc gaaggctgtg cccccatcgt tcgcgcgttc gagcgcggcg acgagcatgc
     2041 cgagctgtgg gagaacgcgc acacggcggc agccggtatc cgggtgcccg ccgccgtggg
     2101 cgactttctc attctgcgcg ccgtgcgcga gtccgggggc gctgcccttg cggtgagcga
     2161 tgcagacctc ctcgctgccc agcggcgcat tgcggcgcag gagggtctgc tgctctgccc
     2221 cgagggcgct gcaacctacg tcgcctggcg ccaggccctg gaggcgggcc tggtcggcgc
     2281 cgaggacgag gtcgttctct tcaactgcgc cacggggctg aagtatccca tgcccgacga
     2341 gagccggacc ttcaccaggc tcgacgacct catcggctga tcccggcggg cgacccgccc
     2401 gccgttcagg cgccccgacg cccttgggcg atccagcacg cgccccccag caggacgcct
     2461 gaggggccgg cgaagggcgc caaagcctcc cgcacggcgg ccagcgcggc ctcccgcaga
     2521 tccgcagcca gctccttgag gacgttgctc aacggtccga cccctgtcac gaactccatg
     2581 gcctctgtca ggtctgcacc caggcgcatc tcgggattgt ggggtgtgat ggccacgtct
     2641 tcgaacccgg catcggccag caccgaagcc agataatccg gatcggcgaa ggcaaacggc
     2701 cccggcgctc gggggtcggg cgtctccgcc ggcggcagga agggctgcgc ggcggccatg
     2761 ccgatgctca gccagggatt ctccccaggg gcccgccagc agatgaaaca caggcgcccc
     2821 ccggggttca gcgcgccccg caggttggcg aaagccgcta ccggatcggc gaagaacatc
     2881 accccgaacc gggagaacag gagatcgaaa ccgggatcga aggggtggct ctgggcatcc
     2941 gcttcgatga agctcaagga ctcgtcgtcg gcgcgttgtc gtgcccgggc gagcatgggc
     3001 gcggatacgt ccacaccggt cacccgagct ccgaccgctg ccatggccct cgacgtggcg
     3061 ccgcaaccgc acccgacgtc caggacggcc tcgccggggc tcacggccgc ggcctggagt
     3121 atggcggtgg taatgggggc cagcagctcg tccatgcgtt cctctgcggc cacccagttg
     3181 ctggctgtgg gcccgttcca gaattcgatc tgatcggcgt tgtcttttgg catgtctatt
     3241 tcgtcagtgt gtttccggtg ggtgcggggc taaaccgcac ggcgaagaga gattctagaa
     3301 gcacgccgcc gcccggtcac cctgcccagg gatgcccgcc ttttttcgga gtccctcaat
     3361 ggcccggtgc gcgggctcgg gtaaactcga ggcaagggcg atgccggcgg ggtgatcaga
     3421 agcgatggca gggggatcta ccgaacgtgg ggacgagcgc gacgcgctcg ttgcgttgat
     3481 cgagagtgaa gtggcggata ccgccgatta cacgggccgc tcgaagctgc gacccgccgt
     3541 gctcgaagcc atgtgcgccg tgccgcggga agagttcgtg cctccggagg aagcctacgc
     3601 cgcctacgcc aacgtgccgc tgcccatcgg acacttccag acgatctcgc agccatacgt
     3661 cgtcgccctc atgaccgatc tgctggatcc ggagcccggc gacgtgatgc tggaagtcgg
     3721 cacggggagc ggataccagg cggcggtgct gagcaccctg gtcagccgcg tctactccct
     3781 ggaggtggtt ccccagctcg ccgccagcgc cgccgagcgg ctggcccggc tgggatacga
     3841 caacgtggtc acccgggagg gcgatggcaa tctgggctgg ccggaccacg cgccctacga
     3901 cggcatcgtg gtgaccgccg gtgctccgga agttccgccg gcattgatcg atcagctcaa
     3961 gcccggcgcg gccctcgtga ttccggtgga cgccgggttc aaccagatgc tcaggcggat
     4021 tacccgcgat cccgacggcg acgtccacac ggaggacgtc ctgccggtgg cattcgtgcc
     4081 gctcgtgaaa gcgacctagg aggggtccgc gcaaccttag cttcctgcga cgtcatggat
     4141 ggccgaccaa tggcgtatca tcttttccca tggcaggcgt ctggcaggaa cttgaccgca
     4201 tcgtgcggcc tcagggacag gttttctatg gctggtggat cgtgctggcc agcagcggtg
     4261 tccaatggct ggccgccgtg ctttggatgc agtcctacgg ggcttacatg gtgctccttc
     4321 agcaggattt cgggtggagc aaagccatgg ttgcgggggc gtttgccctg acccggatcg
     4381 agagcggcat cctcggaccg ctccagggct ggctggtgga ccgattcggt ccccgcgcga
     4441 tcctcaccat cggcacggtg ctcttcgggg tcgggttcat cctgttcggc caggtggagt
     4501 ccctgctgac cttctatctg acgttcgcgc tcatcgcgct cggcgcgagc ctgggaggtt
     4561 tcgccacgtt gatggtggcc atcgtcaact ggttcagtcg ccaccggtcg aaggccatcg
     4621 cggtatccca gctcggcttc tccctcggcg ggctctcggt gccgctgatc atctgggcgc
     4681 tggaggcgtt cggctggcgc gccaccgccg tggtgtccgg cctgctggtg ctcgtcgtcg
     4741 gccttcctct ggtacaggtg gtgcgccatc gaccggaggc tcagggggag ctggcagacg
     4801 ggcacgcggc ctccggcggc ggtccggagg gacgcttctc cctgggcggc gacttcacgg
     4861 ccagacaagc catgcggacg cgctcgttct ggctgatctc cttcggtcac gcgctggccc
     4921 tgctcacggt ctcggccatg atggttcacc tggtgcctca tctgaccgag ggcatgggct
     4981 actcgctgtc ctccgcgggc atggtggtag ccgtgctcac cgcctttcag cttctcggtc
     5041 agctctccgg cggctatctc ggcgatctgt tcaacaagcg cctgatctgt ggggtctgca
     5101 tgttcgctca tgccggcggg ctcctgctcg tcacctacgc ggactccata gtgggcgtgc
     5161 tggcgttcac gctgctccac ggccttgcct ggggcacccg agggcccctc atggtctcgc
     5221 tgcgggccga ttatttcggt cccacctctt tcggcaccat catgggcttc tcgtctctga
     5281 tcgtgatgct gggaatgtcg ggcgggccga tcatcgcggg catcatggcc gacgtttccg
     5341 gcgactatca cagcgggttc accctgctgg ccagcctgtc gttcatgggt tcgctgtgct
     5401 tctttgccgc cacgcctccc agcccgcctg cacgcgcatc ggcatgacgc tcgggccgga
     5461 agctgggtat gctcccatcc tttctcgatg attggtgagg agcgggccaa tgcctgagtt
     5521 tcagggtttt ccgccccgga cgttgaagtt tctgaaggcc ctggaacgca acaacgaccg
     5581 ggactggttc cgggagaaca aggcgctcta cgagagggac gttcttgacc cgtcgctgga
     5641 tttcatcgcc accatgggcc ccaagctggc cgccatatcc gaccatttcg tggcgatccc
     5701 caagcggacg ggcgggtcgc tcatgcgcgt ctatcgggat acccgcttct ctaaagacaa
     5761 gacgccgtat aagaccaaca tcgggatcca gttccgtcac gagctcggca aagacgttca
     5821 cgcgcccggt tactatctgc acatcgacac cgccggatgc ttcgttgccg cgggcatgtg
     5881 gcgtcctgat gcgccgtcgc ttgcccgcgt gcgggagctc atcgccgagc ggcccgggga
     5941 ctggcagaag gcgcgtgacg cccgcccctt caaggcggca ttcgagctag ggggcgagtc
     6001 gctgaagcgg ccgccgaaag gcttcgcgcc ggacgcgccc catctgcagg atctcaggcg
     6061 caaggacttc attgccagcg cgaacgttgc aaccggcgag cttggccagc cgcagcttgt
     6121 cgatgtcgtg gcggagcttt acgcgagggc gacgcccttc atgcgcttcc tgtgccgggc
     6181 catagaggtc gagttctgac gcctacgccc gatggcttgg cggcccggcg gtcgccgccg
     6241 ggctctcccg atccgctctg cggaatcgat cgccgggatc tttaccggat gctaaggtgg
     6301 ccgatgctcc ggggcggtgg cggtgtcagc cgagccggtg cgcagcgtgg gatttcaggc
     6361 cggtcaacga aaggggggat gatgaacaac acgcgattgc tggtggcgct ggcatggctg
     6421 agcctggctc cggcggggtg ggcggaagac gaatgcacgc cctcgcgctg ggggagcgat
     6481 gacgagatcg gcaacgtcaa tctcatcacg acccagtcgg tgctggcggc gtcccggctc
     6541 atcaagacgg ggaagaccta cagcctcggc atcgtggtgg acggcaacac gccggcgttc
     6601 ccgccgcggt cgttgtcgct gcagatcgtt cagcccgggc agcaggaggg taaccggcct
     6661 ctgtcgcgca tgacctacaa cgacgacgtg ttccagggct ggttcggtat cggttcccag
     6721 ctcgacggcc tgggccacgc cggtgacgag ggtatgtact acaactgcaa ccatgcccgg
     6781 gacttctccg acatcgccgg gttgaccaag ttgggaatcg agaaggttcc gcccatcgtt
     6841 gcccggggca tcgtgctgga catgacggcc catttcggcg tcgagcatct ggctggcgga
     6901 cagacgttca gcgtggacga cgtccgcgcg gtggagaaga agcagggcac acccgttcgc
     6961 gagggtgacg tcgtgctgtt tcatacgggg tggacggacg ccatgctcga gacggaccca
     7021 gccggctggt cggcctccgc cccgggaatt tccaaagcgg ttgccggcta cctcgccggc
     7081 aggaacgtgg ttgccgtcgg gtctgacacg tgggggctcg acgttctgcc gcccgagcgc
     7141 gaggacgaac ccttcatcag ccacctgatc cttctgcagc agaacgggat ctacattctc
     7201 gagaccatga acacgggcgc tctcgtccgc gacgaagccc acgagtttct cttcgtgctc
     7261 ggccaggcga aggtccgggg tgcggtccag atgttcatca atcccatcgc cattcgctga
     7321 cgacggcggt cgcgaaggag gagcttgatg acggtggagg tcaacgggat cgcgcacatc
     7381 cagctgacgg ttcgggatcc agcgcgctgc gtgccgttct gggagcggtt gtgtcacttt
     7441 ctggagatga gcacgctcat ccgggcggaa gagatcgtct actgcatcgg cagccgcacg
     7501 ggcgtcctcg tgaggggcgc ccccgaggac aagcgcgacc gggcattcga ccaggatcgc
     7561 ccgggccttc atcatctgtg tttccgggcc cgctcccggg aggacgttga cgccatctac
     7621 gagttcgttg ccggcgagct ggacgcgcac gtcgtgcatc ctcccgagga cggctctcac
     7681 tttgctcccg gatactactc cgtgttgttc gaggatcccg acgggattcg ggtggaggtc
     7741 aacttcgtgc caggccgcgg gcacttcggg tcagaggggc gtctgggtcc cggcggccgg
     7801 ggaccggcga gccgctatgg ggaagagggt ctgggagacg gctagcggcg catgcggcca
     7861 cgtcgccgca tcggcacgac gctcgaccga gct
//
LOCUS       DBNHOX010000730        11573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_573860, whole genome shotgun sequence.
ACCESSION   DBNHOX010000730 DBNHOX010000000
VERSION     DBNHOX010000730.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11573
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_573860"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /locus_tag="AC16UZ_15845"
     CDS             <1..1487
                     /locus_tag="AC16UZ_15845"
                     /inference="COORDINATES: protein motif:HMM:NF015746.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HIR6248210.1"
                     /translation="TQSQQQPQPNNLFFLLLLAVWALSALLSQLDYGHVDPRSGDPVE
                     VRNLLTGEALTSFFAGMVETFVGFHPLGVVLLAMLGIGVADHTGFIKAGLRRLLSLTS
                     QRLLTPMLFLIGILSHSAVDAGYVLVIPLGGVIFQAAGRHPLAGIAVAFAGVSGGFSA
                     NFLPSALDPMLQGISQAGAQLMDPGLVLNPLNNWFFTSASTLLVVLLGWYLTARIVEP
                     RLADEPLDGDLADAPALDDLSDEERRGLRAGLVAMLLGIALLAVTAWPDSSAWRSGGQ
                     LTAAGAPLMQSIVPLIFLLFIIPGVVYGYASGTVRGHRDVIAGMSQAMSGMGYYIVMA
                     FFASLFIAEFGRSNLGVLVALQGAAWLEAAAMPAAVTVVGVVAITGVVNLFVGSASAK
                     WALLAPVLVPMLMALGISPDLAQAAYRVGDSSTNIITPLMPYFPLVVVFAQRYVTRTG
                     IGTIVAMMLPYSVGFLVVWTLFLLVYWMLGIPLGLQASYGYPGG"
     gene            1573..2427
                     /locus_tag="AC16UZ_15850"
     CDS             1573..2427
                     /locus_tag="AC16UZ_15850"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6248211.1"
                     /translation="MSLFRIALRVIVLLALPVWAVAGTLDEIAASGEFRLAYRADTPP
                     FSSRGKGGEPEGFTIDLCRIIARDVQAALNLDEMKITWVPVTAENRFDAIVSGRAHLE
                     CGSSTVTLSRQERVDFSNLTYVTGASLMRWADSDIDRVGDLDGRRVSAIAGTTTEKVL
                     KETLERDGIDAKVVVVGDHAEALQLLLDRKIDAMAGDQATLFGIGFKTQGDDDLVITE
                     DLLSFEPYALPLRRNDADFRLAVNRSLSELYVRGDVGRSWEKWFGSHGVRPTRLLLML
                     YRLNSFSE"
     gene            complement(2468..3400)
                     /locus_tag="AC16UZ_15855"
     CDS             complement(2468..3400)
                     /locus_tag="AC16UZ_15855"
                     /inference="COORDINATES: protein motif:HMM:NF013954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIR6248212.1"
                     /translation="MAGNINRFARAPCAALLLMAAACSEAPAPKPDRSYEFESHTDLE
                     ALFAELNYTPEAWADGNRDVPRLYLTSIPSRWRHRTADSLPVTRKKQLFFRLISPAAL
                     RANELVAADRARLLELAKGPGRLAPDDADWLANLAARYRLRDESAAGPEAIEELMLRV
                     DVLPPSLILAQAAEESGWGTSRFADVGNALFGQWAWDDGIIPLERREELGEYYVARFE
                     TPLDSIRAYMRNLNTHRAYRRLRARRAELRAMGELPSGWELARTLTSYSERGADYVRT
                     LHSIMKANNLDAVDDAYLRDMEPIALVPVGDGAH"
     gene            complement(3413..3967)
                     /locus_tag="AC16UZ_15860"
     CDS             complement(3413..3967)
                     /locus_tag="AC16UZ_15860"
                     /inference="COORDINATES: protein motif:HMM:NF024978.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HIR6248213.1"
                     /translation="MRRCQWGWAVALVLTCAGCAAVAPIGADSDYDSSANFTSYRSFG
                     WISDKPLLLTDATLTSPQFQGRAMKAIRQALEARGYGFEADVENADFAVAFTLGTREQ
                     IRVNAYPDQVRETWTWGGPVPQAADVRNYTEGTLSIDLFDVRLRRPVWHGWATRTISY
                     ADRTNPSPVIAEVVEAILAQFPPQ"
     gene            4080..4979
                     /locus_tag="AC16UZ_15865"
     CDS             4080..4979
                     /locus_tag="AC16UZ_15865"
                     /inference="COORDINATES: protein motif:HMM:NF024946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HIR6248214.1"
                     /translation="MGERSIFSVCCLILLTTQAARANDVEPRLYSAVPTGANFLSVGY
                     VRSEGEVSLDSSIPVIDAKGEVDSLVLQYSRGLNVAGRSGLLSVVVPYANLALEGIYL
                     GQPASGEVRGFGDPVVRLAVNFTGAPAMTGDEFRGYQQKTIVGASVAVSVPVGRYETE
                     RVLNTSSNRWNVVGQVGFSHRVRRWTLEGAVGLSAFSDNDELKGDNELSQESITLFRG
                     TALYHFTPRVWMSLGVVHSRGGATSLNGVKRRDHQNNWRSGLAFSFPVGRRHRMQLVA
                     TEGVSARIGADFTTFIANYTLSF"
     gene            complement(5034..7250)
                     /locus_tag="AC16UZ_15870"
     CDS             complement(5034..7250)
                     /locus_tag="AC16UZ_15870"
                     /inference="COORDINATES: protein motif:HMM:NF025875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6248215.1"
                     /translation="MVPIRAALLPLIVSFLSGCDARPPVATDTPDHELEPSTAALTYV
                     GSQQCAGCHAGQHDAWQRSHHRKAMEKPGSHSIVGNFDGASFVHDGTTTEFRAESGSY
                     VVYTDGENGEIARFPVAYTFGVEPLQQYLLPLPRGRLQALSVAWDSRPREAGGQRWFH
                     LYPEDRVDHTDVLHWTQGSQNWNYMCADCHSTNVRKGYDETLDEYDTSFSEVTVGCEA
                     CHGPASAHVDWALTGDGQAGLPSLGGQTAEINACAPCHSRRSQIAEGFRPDLAYLDHY
                     VPALLDEGLYHSDGQILDEVYVYGSFLQSRMHAQGVSCSNCHDPHSAELRATGNDVCT
                     GCHSEAGRADFPTLTKARYDASAHHFHSDGGPGSQCVDCHMPARTYMQVDDRRDHSFR
                     VPRPDLTVELGVPNACTGCHEDRPASWARDVLAERFGKAEREHYGAVFDAARRADPSA
                     ERALAALAEDEARPAIVRATALSLMTAYDRGASSFALSAGLRDESPLVRLGALKGAER
                     WPPDRRWRSAAHLLEDDYLAVRLEAVRVLAPAAGSLPAASRERLNRGVSEYASAQALH
                     GDRAEAHTNMAGVYLNLGEPSLAERALSRAIQINPRWVPALINLADLYRATGRDAAGG
                     GFLDRALSAAPDSGDVLLAKALWLVRQRSQDEALTLLARAAAVEPTSTRIAYVYAAGL
                     HSNDRTQEALEVLDTILATRPGNRQLLEAAAAMARDNGQDGRALGYLEQLKAADGL"
     gene            complement(7348..8061)
                     /locus_tag="AC16UZ_15875"
     CDS             complement(7348..8061)
                     /locus_tag="AC16UZ_15875"
                     /inference="COORDINATES: protein motif:HMM:NF023197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HIR6248216.1"
                     /translation="MKKNSRIARFALPLAVLLLGACASTTPTINTEAAPDEMSYDGLY
                     PVKGTRVTQAWARSDLDLRSYNKILLQGAGIQYRPVKSTGYRGTAGRSGKNEFPLDEQ
                     QKERVAAAASEAFRSELAKIKSLEIVSEPGPDVLIVRGALLDVVSRVPPEPIGRGNTY
                     LDSVGEATLVIELADSQSGSVLVRAVDRRAAQQTGWMTESTSVTNAAEVRRLFSGWAR
                     LVRTALEELTDDLDIGEAQ"
     gene            8498..10921
                     /locus_tag="AC16UZ_15880"
     CDS             8498..10921
                     /locus_tag="AC16UZ_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248217.1"
                     /translation="MDVEGGLVVIHAPQIRAWDDFEDIEGLAALELRTDDGAPGRFGT
                     IAFRARAVPDLEARVVHFDDIEVTSVKEQGEEISEEARALFVAAMDLGARDVPLDLVL
                     SHLADGVIPESTTGIRFEPPEILVSTAPAVIVLLHGEPALAPVGDRGALFAANTNWPL
                     FRNGAEGAWYLRDNDNWLTAADLDGPWAWAGELPASLVQLPETDDWRSTRAAAKAWSR
                     PPESEPPSVFVSTEPAELILIDGEPELVEIGTLGLSFVANTESPLFGYSERWYYLVSG
                     RWFTSRALDGRWSAVLELPKAFAQIPEDHEKADVLAAVPDTPAARMAALDAQIPEKTE
                     VPRDLSMPETVTYVGEPVFEPIPGTNLKRATNTAYSVIQAGADYYLCYSGMWYAASAP
                     EGPWRVTSHVPSQVYQIPPSSPVYNTTYVKVQEESPVSVTFGVTQGYSSVYVSNGVPV
                     YGTGWYYPPYYYYYGGYPIYFAYPYTYGSASFYNPATGTYGSVSRAYGPYGGWGYASA
                     YNPTTGTYGRAEAVWDHDEWYAVGEAYNPHTGRYYTTERYYDADDERWEIDSRFSGER
                     GYVDVSREFDEDSGRATIETSRGGEGTFSREKSDGGWNTSGEFETAGGRTVTGSGRYE
                     DGEGSSTLRGSEGGTGTIERSVDDGVVTRDGTFSKDGKTIETETTRDGTGRRTSFETS
                     EGTSGAVGGRGQQRTALAQTESGDLYAARDGNVYRKSDDGWSRHSDGSWETIPMPSRD
                     ADAGARSYGGGDYRQSASSAYRGGADLDADRYRGLDRDYSARQRGMTRAGSYGGQRGS
                     FGRLQGRRR"
     gene            complement(10992..11375)
                     /locus_tag="AC16UZ_15885"
     CDS             complement(10992..11375)
                     /locus_tag="AC16UZ_15885"
                     /inference="COORDINATES: protein motif:HMM:NF028308.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HIR6248218.1"
                     /translation="MAQRHSRILTLLLLVVVAAPATAFWGWMRGSAANEFKESDWDLF
                     KAEMRQALEDGADGERFDWSNPKTGNSGSIRILETAEVEGETCRKAAFRNVTAKGTQG
                     QSVHYLCRQENGAWQFAAASAFESG"
BASE COUNT         1842 a         3754 c         3875 g         2102 t
ORIGIN      
        1 acacacaatc ccaacaacaa ccacaaccga acaacctgtt cttcttgctc ctgctggcgg
       61 tctgggcgct atcggcgctg ctctcgcagc tcgactacgg tcacgtcgac cctcgcagcg
      121 gggatccggt ggaggttcgc aacctgctga ccggcgaggc cctgaccagc ttcttcgccg
      181 gcatggtcga gacgttcgtg gggtttcacc cgctgggcgt cgttctcctc gccatgctcg
      241 gtataggggt ggccgaccac accggcttca tcaaggcggg gctgcgccgg ctgctcagcc
      301 tgacttcgca gaggctgctc accccgatgc tctttctgat cggcattctc agccacagcg
      361 ccgtggatgc cggatacgtg ctcgtcatac ccctgggcgg cgtcatcttt caggcggctg
      421 gaagacaccc tctggcggga atcgcggtgg ccttcgccgg cgtctccgga gggttcagcg
      481 cgaactttct gccctccgcc ttagatccca tgctgcaggg catcagccag gccggcgcgc
      541 agctgatgga tccgggcctg gtgctgaacc cgctcaacaa ctggttcttc acctccgcct
      601 ccacgctgct cgtcgtgctt ctcggatggt acctgacggc tcggatcgtg gagccgcggc
      661 tggctgacga gcccctggac ggcgatctgg ccgatgcgcc cgccctggat gatctttccg
      721 acgaagagcg tcgcgggcta cgcgcggggc ttgtcgccat gctgctcgga attgcgctgc
      781 tggcggtcac ggcctggccg gacagttcgg catggcgctc gggcggccag ctgaccgccg
      841 cgggtgcgcc cctcatgcag agcatcgttc cgctcatctt tctgctcttc atcattcccg
      901 gcgtggtcta cggttacgcc tccggcacgg ttcgggggca tcgggacgtg atcgcgggca
      961 tgagccaggc catgagcggg atgggttact acatcgtgat ggccttcttc gcttcgctgt
     1021 tcatcgccga gttcggacgg tccaacctgg gcgtgctcgt cgcgctgcag ggagcggcct
     1081 ggctcgaggc ggccgccatg ccggcggcag tcaccgtggt gggggtggtg gccatcaccg
     1141 gtgtggtgaa cctcttcgtc ggctcggcct cagcgaagtg ggcgctactc gcgccagtgc
     1201 tggttccgat gctgatggct ctgggcatct cgccggatct cgcccaggcc gcctaccgag
     1261 taggcgactc ctcgacgaac atcatcacgc cgctgatgcc ctactttccc ctggtggtgg
     1321 tcttcgcgca gcgctacgtg acgcgcacgg gcatcggcac catcgtggcg atgatgctgc
     1381 cttactcagt gggcttcctc gtggtgtgga cgcttttcct gctggtttac tggatgcttg
     1441 gaattccgct gggcttgcag gcgagctacg gctatcccgg gggttgagcc cgatcgtcgc
     1501 cgtaggggac ccgccggccg tgatcggcta gagtaggtgg ctgatcagaa tcattggaat
     1561 cggggtcttc tcatgagctt atttcgcatc gcgttgcggg taatcgtgtt gctggccttg
     1621 ccggtttggg ccgtggcggg aaccctggac gagatagccg cctcgggcga gttccggctt
     1681 gcctaccgag cggacacgcc gccgttttcg tcgcgcggca agggcggcga gcccgagggc
     1741 ttcaccatcg acctgtgccg catcattgcc cgtgacgtcc aggcagcgct gaacctcgac
     1801 gagatgaaaa tcacctgggt gcctgtgacg gcggagaacc gattcgatgc catcgtctcc
     1861 ggccgggcgc atctggagtg cggctcctcc acggtcaccc tgtcccggca ggagcgcgtc
     1921 gacttctcca acctgaccta tgtgaccggc gccagcctca tgcgctgggc ggactcggac
     1981 atagatcggg tcggtgatct ggacggtcgg cgcgtttcag ccatcgcggg cacgaccacg
     2041 gagaaggtcc tcaaggaaac cctcgagcga gatgggattg atgccaaggt ggtggtcgtg
     2101 ggtgatcacg cggaagcgct gcagctgctg ctggacagaa agatcgatgc catggctggc
     2161 gatcaggcca cgctcttcgg catcggattc aagacccagg gtgatgacga cctggtgatc
     2221 accgaggatc ttctctcctt tgagccatac gctctgccgc tcaggcgcaa cgacgccgac
     2281 tttcgcctgg cggtcaaccg ctcgctctcc gagctctacg ttcgaggtga cgtgggtcgg
     2341 tcctgggaga agtggttcgg ctcccacggg gtgcgcccga cgcggctact gctgatgctc
     2401 taccgcctca actccttctc ggagtagggc gcgcgacggc tcgtcaggcg gccgcccgct
     2461 gggctgctca gtgcgccccg tcgcccacgg gaaccagcgc gatgggctcc atgtcgcgca
     2521 ggtaggcatc gtccacggcg tccaggttgt tcgccttcat gatcgagtgc agggtacgga
     2581 cgtagtccgc tccccgttcc gagtatgagg tcagggtgcg ggccagctcc cagccgctgg
     2641 gaagctcacc catggcacgc agttccgctc ggcgtgcgcg caggcgccgg taggcccggt
     2701 gcgtgttcag attccgcatg tacgcgcgta tggaatccag cggtgtctcg aagcgagcga
     2761 cgtagtactc gcccaactcc tcacggcgtt ccagaggaat gatgccgtcg tcccatgccc
     2821 attggccgaa cagggcgttg cccacgtcgg cgaagcgcga cgtgccccag ccgctctcct
     2881 ctgcggcctg agccaggatc agagatgggg gcagaacgtc cacgcgcagc atcagctcct
     2941 cgatggcctc cggcccggcg gcactttcgt ccctcagccg gtaacgggcg gccagattcg
     3001 ccagccagtc ggcatcatcc ggcgccagcc gcccggggcc cttggccaac tcgagcagcc
     3061 gagctcgatc agcggcgacc agctcgtttg cccgcagcgc ggcaggactg atcaggcgga
     3121 agaaaagctg tttcttccgg gtgacgggga ggctgtcggc ggttcgatgc cgccatcggc
     3181 tcgggatgct ggtgaggtac agtcggggca catcccgatt gccgtccgcc caggcttccg
     3241 gcgtgtagtt cagctctgcg aacagcgcct cgaggtccgt gtgggattcg aactcatagc
     3301 ttctgtccgg ctttggagcc ggcgcctcgg agcaggcggc ggccatcaac agcagcgctg
     3361 cgcaaggtgc ccgggcgaaa cgattgatgt tgcctgccat tgccggttcc ggtcattgcg
     3421 gcggaaactg cgccaggatc gcctcgacga cctcggcgat caccggcgac gggttcgtcc
     3481 gatcggcgta ggagatcgtc ctggtcgccc acccgtgcca tacggggcgg cgcaaacgca
     3541 cgtcgaacag gtcgatggac agggtgccct cggtatagtt ccgcacgtcc gcggcttgag
     3601 gcacgggccc gccccaggtc caggtttccc gaacctggtc cgggtaggcg ttcacacgaa
     3661 tctgctcgcg cgttcccaac gtaaaagcga ccgcgaaatc ggcgttctcg acatcggcct
     3721 cgaagccata gcccctggct tccagagcct gcctgatggc cttcattgcc cgtccctgaa
     3781 actgcgggct cgtcaaggtc gcgtcggtca gcagcagcgg cttgtcggat atccatccga
     3841 agctccggta ggaggtgaaa ttcgcgctgc tgtcgtagtc gctgtcggcg ccgatgggcg
     3901 cgacggctgc gcacccggcg cacgtcagga ccaaggcgac ggcccagccc cattggcatc
     3961 gtctcatttc ggtgtcctcc cgtggatccc gtcgcggcgt ccgcgcgccg tttccagaac
     4021 gccgatggta gcagttttcg ctatgatcac gggcctgcac cgttaccggc gaggctggct
     4081 tgggagagcg ctccatcttc agcgtttgct gcctgatcct gctgacgacc caggcggccc
     4141 gcgcaaacga cgtcgagccg cgcctgtact cggcagtacc cacgggggcc aatttcctct
     4201 cggtgggcta cgtgcgatcc gagggtgaag tgtctctgga cagcagcatt cccgtgatcg
     4261 atgcgaaagg agaggtggac tccctggtcc tccagtactc gcgagggttg aacgtcgccg
     4321 gcagatcggg gctgctcagc gtggtggtgc catatgccaa cctggcgctc gaggggatat
     4381 accttgggca gccggcgtcc ggcgaggtac gcggttttgg agaccccgtc gtgcgtctgg
     4441 cggtgaactt caccggcgcg ccggccatga ccggggacga gttccgcggc taccagcaga
     4501 agaccatcgt cggtgccagc gttgccgtct cggtgccggt ggggcgctac gaaaccgagc
     4561 gtgtcctcaa cacgagcagc aatcgctgga acgtcgtcgg ccaggtgggc ttctcccatc
     4621 gggttcgtcg gtggaccctg gaaggcgccg tcggcctatc ggcgttctcc gacaacgacg
     4681 agctcaaggg cgacaacgag ctgtctcagg agtccataac gctgttccgc ggcaccgcgc
     4741 tgtatcactt caccccgcgg gtctggatga gccttggcgt ggtacacagc cgcggaggtg
     4801 ccaccagcct caacggtgta aagcgtcgcg atcatcagaa caactggcgc tcgggcctgg
     4861 ctttctcgtt ccccgttggc cgcagacaca ggatgcagct ggtggccacg gaaggggtca
     4921 gcgcgcgaat cggcgccgac ttcaccactt tcatcgcgaa ctacaccctc tctttctgag
     4981 tcctctgggg agccgttggc gcgcgtaaga tccgccaggc gggcagggtt cgctcagaga
     5041 ccgtcggctg cttttagctg ctccaggtat ccgagcgctc ggccatcctg gccgttatcc
     5101 cgggccatgg ccgctgctgc ttcgagcagc tgtcggttgc cagggcgggt ggcgaggatg
     5161 gtgtcgagca cctccagggc ctcctgcgtt cggtcgttcg agtgaagccc ggcggcgtac
     5221 acgtaggcga ttcgggtgct ggtcggctcg acggctgctg cgcgggcaag cagggtgagg
     5281 gcctcgtcct gagaccgttg ccgcaccagc cacagcgcct tggcgagcag cacgtcccca
     5341 gagtcgggtg ctgcgctgag cgcacggtcc agaaacccgc cccccgcggc atcacgcccc
     5401 gtcgctctgt acaggtcggc caggttgatc agagccggca cccaccgggg attgatctgg
     5461 atggcccggg atagcgcccg ttcggccagg ctcggttcgc ccaggttcag atagacgcca
     5521 gccatgttcg tgtgcgcctc cgcccgatcc ccatgcagcg cctgagcgga agcgtattcc
     5581 gacacaccgc gattcaggcg ttctcgtgat gccgccggga gcgagcctgc tgccggggcc
     5641 agcacgcgga cggcctccag ccgcacggcg aggtagtcgt cctccagcag gtgcgccgcc
     5701 gaacgccagc ggcgatcggg cggccagcgc tcggcccctt tcagggcacc gaggcgcacc
     5761 agggggcttt cgtccctcaa gccggcgctc agcgcaaacg agctggcgcc acggtcgtaa
     5821 gcggtcatca gggaaagcgc ggtggcgcga acgatggccg gccgggcctc atcctcggcc
     5881 agcgcggcga gcgctcgctc tgcgctcgga tccgcgcgac gggccgcgtc gaaaacggcg
     5941 ccgtagtgct cgcgttcagc cttgccaaag cgctctgcca acacgtccct cgcccaactg
     6001 gccgggcgat cctcgtggca gccggtgcag gcgttcggca cacccagctc gacggtcaga
     6061 tcaggccggg gaacccggaa gctgtgatcc ctccgatcgt cgacctgcat gtaggtgcgg
     6121 gcaggcatgt ggcagtccac gcattgcgag ccagggcctc catccgaatg aaaatggtgg
     6181 gcggatgcat cgtagcgagc ctttgtgagc gtggggaagt cggcccggcc cgcttcggag
     6241 tggcacccag tgcacacgtc gttgccagtg gcgcgcagct cggccgagtg cgggtcgtgg
     6301 cagttgctgc agctaacccc ttgtgcatgc atcctgctct gcaggaacga tccgtagacg
     6361 tacacctcgt cgagaatctg cccgtcgctg tggtagagcc cctcgtccag cagggcggga
     6421 acgtagtgat cgaggtaggc gagatccggc cggaacccct cggcgatctg gctgcgtcgg
     6481 ctgtgacagg gcgcgcaggc gttgatctcg gcggtctgac ccccaaggct gggaaggccg
     6541 gcttggccgt caccggttag cgcccagtcg acgtgcgcgg acgccgggcc atggcaggcc
     6601 tcgcagccta cggttacctc gctgaagctc gtgtcgtact catccagcgt ctcgtcgtaa
     6661 cccttgcgta cgttggtgga gtgacagtcg gcgcacatgt agttccagtt ctggctcccc
     6721 tgggtccagt gcagaacgtc cgtgtgatcc acccgatcct cgggatagag gtggaaccag
     6781 cgttgcccgc cggcttcccg gggccgcgag tcccaggcca cgctcaacgc ctgcaggcga
     6841 ccgcgcggga gaggcagcag atactgttgc agcggctcga cgccgaacgt gtaggcgacg
     6901 gggaagcggg cgatctcgcc gttctcgcca tcggtgtaca ccacgtagga acccgattcc
     6961 gcgcgaaact cggtggtggt cccgtcatga acgaacgagg cgccgtcgaa attgccgacg
     7021 atgctgtggg agcctggctt ttccatggct ttgcgatggt gcgagcgctg ccaggcgtca
     7081 tgctggccgg cgtgacagcc cgcgcattgc tgggagccaa cgtatgtgag cgccgccgtc
     7141 gagggctcga gttcgtggtc cggggtgtca gtggcgaccg gtgggcgggc atcgcagccc
     7201 gacaggaagc tcacaatcag gggcaggaga gcggctcgga tcgggaccat ggcaggcgac
     7261 ggggtcagcg agcgttaacc gtcacccatc gccccgggag gacgcgatgc gcctgaaccc
     7321 ggggcgagag ggcggaagat ggctcagcta ttgggcttcg ccgatgtcca gatcgtcggt
     7381 cagctcctcc agggccgtgc gcaccaggcg agcccaaccg ctgaacagac gccgcacttc
     7441 cgcggcgttg gttaccgagg tggactccgt catccagccg gtctgctggg cggcgcgccg
     7501 atccacggcg cgcaccagca cgctgccgct ttgggaatcc gccagctcta tgaccagcgt
     7561 cgcctcgccg acagaatcca ggtaggtgtt gcctcgacca atgggttccg gcggtacccg
     7621 ggacaccacg tcgagcagcg cgccgcgcac gatgagcacg tcgggcccgg gctccgaaac
     7681 gatctccagg cttttgatct tcgcaagctc gctgcggaaa gcttcgcttg cggccgcggc
     7741 gacgcgctct ttctgctgct cgtccagggg aaactcgttc ttgccgctgc gaccggcggt
     7801 gccccggtat ccggtcgact tgaccggtcg atactgaatg cccgctccct gcagaagaat
     7861 cttgttgtag cttctcaggt cgaggtccga gcgggcccag gcctgggtaa cccgggttcc
     7921 cttcacggga tacaggccgt cgtaggacat ctcgtccggg gccgcctcgg tgttgatggt
     7981 tggtgtcgtg ctcgcgcagg cccccagcag caggacggcc agcggcagcg cgaacctggc
     8041 gatgcggctg ttctttttca tcgcggattc cccctttttt gtcattggtg gacggttgct
     8101 ttccatgtcg tgaggggccg atgttagcgt caagctaacc gaaagcaagg cggcctactg
     8161 gcaggtggcc tcgcagcccg cagcctgggc gtcccctggg cagtcaccgc gttcgggttg
     8221 tcgtcgtggg cacgatcacg gctttcgact acactgcccg gtctcctgcg cagacccccc
     8281 gaggaacgtt atggaccgct gggcaccaac aggccttgcg ttgatcagtt cgctgttgct
     8341 tgtgagcgcg tcgcccgtcc cgtcgtggct gatgctgagt tcggctcagg cgtcagagcc
     8401 ggcggctggc ccgtctgcca ccgcgtctgc caccgcgtct gccaccgcgt ctgccggcaa
     8461 gactgaagcg ccgacgccag ggcagtttcc gaggtccgtg gatgtcgagg gcgggttggt
     8521 ggtgattcac gctccgcaga ttcgcgcgtg ggatgatttc gaggacatcg agggactcgc
     8581 ggcgttggag ctgcgcacgg acgacggcgc ccccggccgg tttggcacca tcgcgttcag
     8641 ggcaagggcc gtcccggacc tggaagcccg cgtggtccac tttgacgaca tcgaggtcac
     8701 ctcggtgaag gagcagggcg aagaaatctc cgaggaggca cgggcgctgt tcgtggcggc
     8761 catggatttg ggggcccggg acgttccgct cgacctggtc ctgagccacc tggctgatgg
     8821 ggtgatcccg gagtccacca cgggcatccg cttcgagcct ccagagatac tcgtttccac
     8881 cgccccagcc gtgatcgtcc ttttgcatgg cgagccggcg ctcgcgcctg tgggggatcg
     8941 gggagcgctg tttgctgcca ataccaactg gcccctgttc agaaacggcg ccgagggagc
     9001 ctggtacctg cgggacaacg acaactggct caccgccgcc gatctcgacg gtccgtgggc
     9061 ttgggcgggt gagcttccgg cctctctggt tcagctgccg gaaacggacg actggcgctc
     9121 cacccgcgca gcggctaagg catggtcgcg gccgccggaa agcgagcccc catcggtttt
     9181 cgtatccacc gagccggccg agctgatcct gattgacggc gagccggaac tggtagaaat
     9241 cggcaccctt ggcctgtcct tcgtcgcgaa taccgaaagc ccgctgttcg gctactccga
     9301 gcgctggtac tacctcgttt ccggacgctg gttcacgagc agggcgctcg atggcaggtg
     9361 gtcggccgtg ctcgagttgc ccaaggcgtt tgcccagatt cctgaagatc acgaaaaggc
     9421 tgacgtgctg gccgcggttc ccgatacgcc agcagccaga atggccgctc tggatgccca
     9481 gattcccgag aagaccgagg tgcctcggga tctcagcatg ccggaaaccg tgacctatgt
     9541 gggcgaaccg gtgttcgagc ccatcccggg caccaacctc aagcgtgcga cgaacaccgc
     9601 ctactcggtc attcaggccg gcgcggacta ctacctgtgc tacagcggca tgtggtacgc
     9661 ggcttcggcg cccgaagggc cctggcgcgt gaccagtcac gtgccctccc aggtctacca
     9721 gatcccgccc agctcgccgg tctacaacac cacctacgtg aaggttcaag aggaaagccc
     9781 ggtctcggtg accttcggcg tgacccaggg ctactcgagc gtgtacgtct ccaacggggt
     9841 gcccgtctac ggaaccggct ggtactaccc gccctactac tattactacg gcggctatcc
     9901 gatctacttc gcttatccgt acacgtatgg ctccgcctct ttctacaacc cggccacggg
     9961 cacctacggc agcgtttcga gggcctacgg accttacggc ggctggggtt acgcctcggc
    10021 ttacaacccg accacgggca cttacggtcg ggcggaggcg gtttgggatc acgacgagtg
    10081 gtatgcggtg ggcgaggcct acaaccccca caccgggcgg tactacacaa ccgagcggta
    10141 ctacgatgcc gatgacgagc gctgggaaat cgactcccgg ttctccggcg agcgtggtta
    10201 cgtagacgtg tccagagagt tcgacgaaga ctccggtcgc gccaccattg aaacgtcccg
    10261 gggcggggaa ggcaccttct ctcgcgagaa aagcgacggg ggctggaaca ccagcggcga
    10321 gttcgagacc gccggcggcc gcaccgtcac cggcagcggc cgctacgaag acggcgaagg
    10381 aagctccacg ctgcgtggct ccgaaggggg taccggcacc atagaaaggt cggtggatga
    10441 tggcgtggtg acccgggacg gcaccttttc caaggatggc aagaccatcg agacggaaac
    10501 cacccgcgac gggacgggga ggcgcacgtc gttcgagacc tcagaaggca ccagcggcgc
    10561 ggtcggcggc cggggacagc agcgcacggc gctggcgcag accgagtccg gtgatctgta
    10621 cgcggcgcga gacgggaacg tctacaggaa atcggatgac ggctggtcgc gtcacagcga
    10681 cggcagctgg gagaccatcc cgatgcccag ccgcgacgcc gatgccggcg cccgatccta
    10741 tggcgggggc gattaccgcc agtccgcgtc tagcgcctat cgaggcggcg cggatcttga
    10801 tgccgatcgc tatcgcggcc tggatcgcga ttactccgcc cgacagcgcg gcatgacccg
    10861 cgccggctcg tatgggggac aacgcggcag cttcggtcgg ctgcagggcc ggcgccgtta
    10921 gcaccagccg gtacggcctg gaaatacggt tgctaggtgc cggatcggga gcggtccagg
    10981 ggcgattggg atcagccgga ttcgaacgcg ctggccgcgg cgaactgcca ggcgccattt
    11041 tcctgtcggc aaagatagtg gacggattgc ccctgggtac ccttggcggt cacgttgcgg
    11101 aacgccgcct tgcgacaggt ctcgccttcc acctcggcgg tctcgagaat tctgatcgac
    11161 ccgctgttgc cggttttcgg gttgctccag tcgaagcgct cgccgtcagc gccgtcttcc
    11221 agggcctggc gcatttccgc tttgaacaga tcccagtcgc tctccttgaa ctcgttggcg
    11281 gccgacccgc gcatccagcc ccagaaggcc gtggcaggcg ccgcaacgac gaccagcagg
    11341 agcagggtaa ggattcgcga gtgccgctgc gccatgatgt tttcgacccc gtcgaaaaag
    11401 gagtcaagtc tagcctaagg tcgcggatct gctccaccga tcccggcctg gcagccggcg
    11461 cagcgcttcc ggcggcgagg gggtgaggtg actcgatgcg ccaggtttag ccggtttgcc
    11521 tgtccacgag acccgggtat catgcgggcg gcgcgcgacg gaccgagacg aaa
//
LOCUS       DBNHOX010000731         6917 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_252834, whole genome shotgun sequence.
ACCESSION   DBNHOX010000731 DBNHOX010000000
VERSION     DBNHOX010000731.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6917)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_252834"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="AC16UZ_15890"
     CDS             <1..607
                     /locus_tag="AC16UZ_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248219.1"
                     /translation="LAVPSYTFVRFGMWQEALAVPEPEEDLVIAAFMRHYGRGVAHAA
                     LDDTGSAKAELAAMEAILASGEFRAQMDRQGDIGDILASIASSLVRARIARADGDVDA
                     EIRHLRDAVASQDSLPYSEPPYWHYPVRQSLGSALLRLGDADGAAAAFTEDLGKFPKN
                     GWSLHGLAEARRALGKPTDELEAAHREAWQYADIEPTAGAE"
     gene            632..1891
                     /locus_tag="AC16UZ_15895"
     CDS             632..1891
                     /locus_tag="AC16UZ_15895"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6248220.1"
                     /translation="MSEALAPIDLTTADPYSVPLDQIDMSQAALFQTNTFGPYFERLR
                     REDPVHYTADSFFGPYWSITRFDDIVYVDKHHELFSSEDGITLADRQQDFATVNFIAM
                     DPPKHDDQRKAVQGVVAPRNLAKMEALIRGRVADILDSLPLGETFNWVDRVSIELTTQ
                     MLATLFDFPFEERRKLTYWSDMATSSELAGGPTPEPVRREALMECLEAFTRIWHERAS
                     QPPDESVDLISMLAHSDDTKDMLNRPMEYLGNLILLIVGGNDTTRNSITGGVLALNQN
                     PGEYDKLRQHPELIPNMVAEIIRWQTPLAYMRRTATQDTELGGKLIRKGDKIAMWYVS
                     GNRDETAIDQPEEFIIDRPNARHHLSFGFGIHRCMGNRLAEMQLRIVWEEILKRFHTV
                     EVVGEPVRVQSSFVKGYSQLPVRLHPV"
     gene            complement(1954..2637)
                     /locus_tag="AC16UZ_15900"
     CDS             complement(1954..2637)
                     /locus_tag="AC16UZ_15900"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR6248221.1"
                     /translation="MSTLAVAFTAGGAHAQPDSGASHQHAAPVVLAPGYADLDFAPPP
                     VGGYALPPLGEVADARVVDAQGVIGRLHEMLGDKVVVLSFIYTRCNDVNGCPLATHVL
                     GKLQGAITSAPDLQGKVRLVSFSFDPSHDTPAVLRQYAGHFRKPGFDWRFVTVANAAD
                     LTSVLDAYDQWVVRDIDADGEFVGTLSHILRVYLIDRDRQIRNIYSISFLHADTVAND
                     IRTVLLDGG"
     gene            complement(2662..3999)
                     /gene="mtoX"
                     /locus_tag="AC16UZ_15905"
     CDS             complement(2662..3999)
                     /gene="mtoX"
                     /locus_tag="AC16UZ_15905"
                     /EC_number="1.8.3.4"
                     /inference="COORDINATES: protein motif:HMM:NF052453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA];
                     GO_function: GO:0018549 - methanethiol oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanethiol oxidase"
                     /protein_id="HIR6248222.1"
                     /translation="MKTIRKMLAQLAAGALLAMPVWADETCMSPYMAKIVGQEDFVYV
                     WTLGMEGVGDGQDKLVTVDVNPTSKTYGEVVHSLSVGGRNEAHHSGLTDDRRFLWASG
                     LDTSKIFIFDVHTNPGAPTLHRMIDDFVARSGGVVGPHTNYALPGRMMLTGLSNNRDH
                     GGRTALVEYTNAGDYIATHWIPTDDNLQGAVKSGSYADGYGYDVRVLPRRNVMITSSF
                     TGWNNYMMNLGAMMADAEAMKRFGNTVVVWDLHARQPKKILDVPGAPLEIRCAWGAKS
                     NYCFTSTALTSKIWLIYEDEGGEWQAKAVADIGDAAKIPLPVDISISAEDNLLWVNTW
                     NDGLVHLYDISDPHAPKQLFEQKIGEQVNMVSQSWDGERLYFTSSLLANWDKTGPSGP
                     DLQYFKLYGWDGEKMTPGFTIDFVAAGLGAPHQMRFGSYALYGKRPPQQVGLH"
     gene            4068..5297
                     /locus_tag="AC16UZ_15910"
     CDS             4068..5297
                     /locus_tag="AC16UZ_15910"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR6248223.1"
                     /translation="MWRGPITSQAVAALMLTACCTWAVTPGDPRDGYEHAHYETASRQ
                     LSALRGSALALAELAGNPPLGLPPLPAERLPVAAAIDLGRRLFFDRRLSANATLSCAM
                     CHVPEQGFTQNELKTPVGIEGRFVHRNAPSLYNVAYQQALFHDGREDDLANQIWAPLL
                     AANEMGNASREAVVERLGGLEEYEGEFLRVFGDGISAATLGRALASYQTALLSADSPF
                     DRWYFGGEADAVGAEVKRGFRSFVNEGCATCHQLQTGHATFTDGAYHDTGIGFLAAAS
                     AGTPARRLQIAPGVIIDVETDIATPHVEDDGRAAVTGDPADRWRYRTPGLRNVALSAP
                     YMHDGSLATLEAVIDYYAAGGAPHDGQDARIRPLSLGADERADLVAFLQALTGSNVDA
                     LAGDARAAPIGDTHHGP"
     gene            complement(5313..>6917)
                     /locus_tag="AC16UZ_15915"
     CDS             complement(5313..>6917)
                     /locus_tag="AC16UZ_15915"
                     /inference="COORDINATES: protein motif:HMM:NF039585.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR6248224.1"
                     /translation="TYHRIEEVLDCWFESGSMPYAQLHYPFENEKVFKAGFPAEFIAE
                     GLDQTRGWFYTLTVLAAALYDKPAFRNVIVNGMVMAEDGKKMSKRLRNYTPPDELMET
                     YGADALRLYLINSGLMRGEEQRFADAGVRDMTRRALLPWYNAFTFLKTYAEIDQWSPD
                     KGQHTGDNVLDHWILSRLQTLKANVGREMEVYKLYNVVPQLFDFIEDLTNWYIRLNRQ
                     RFWGEDITADKIAAYSTLYAALYELCQVMAPFAPFLAEHVYQELSALGGGRAKPESVH
                     LCDYPEPEGALIRSELEEAVDRMQQVVLLGRQKREEVKIGLRTPLRRLTIVNRDRALL
                     EELARLESYVKTELNVLAVSYDDDEGAYIEVVAKPNFPVLGKRLGKRMKAFQSAIATL
                     SPEQIAAFQAEGEIELDGEVFNLEEIEVLQKARPGTNTISNRRIAVDLDCVLDDELVR
                     GGYAREIVNRIQRRRKELELNVADRIEVCFAGDRALVEAAAEHRDYIAAETLATDFAE
                     GDPGSQAADSEIDGKAFRFSVVRAGS"
BASE COUNT         1220 a         2325 c         2188 g         1184 t
ORIGIN      
        1 cctcgcggtg ccgtcctaca ccttcgtgcg cttcggcatg tggcaggaag ctctggcggt
       61 tccggaaccc gaggaagatc tggtgatcgc cgcgttcatg cgtcactacg gccgcggggt
      121 cgctcacgcc gccctggacg atacggggtc cgcaaaggcg gagctcgcgg ccatggaggc
      181 aatcctggca tccggcgagt tccgggccca gatggatcgc cagggagaca tcggggacat
      241 cctcgccagc atcgcctcgt ccctcgtgcg cgcccggatc gcaagagccg acggggacgt
      301 ggacgcggag attcggcatc tgcgcgacgc cgtcgcaagc caggactcgc tcccgtacag
      361 cgagccgccc tactggcact accccgtccg ccaatcgctg ggctccgcgc tgctccggct
      421 gggcgatgct gacggggcag cggcggcctt caccgaggat ctcggcaagt ttccgaagaa
      481 cggctggtcc ctgcacgggc tggccgaagc ccgccgcgct ctgggaaagc caaccgacga
      541 actggaggcg gcccaccggg aagcgtggca atatgccgat attgagccaa cggcaggcgc
      601 cgagtagcga gttcaaaacc ggaggttcgt gatgtccgaa gccctagcac cgatcgatct
      661 gaccacagcg gacccgtact cggtgcccct ggatcagatc gacatgagcc aggccgccct
      721 cttccagacc aacaccttcg gcccgtactt cgagcgcctg cgccgggaag atccggtgca
      781 ctacaccgcg gacagttttt tcgggcctta ctggtccatc acccgcttcg acgacatcgt
      841 ctacgtggac aagcaccacg agctcttctc gtccgaggac ggcatcaccc tggccgaccg
      901 ccagcaggac tttgccaccg tcaacttcat cgccatggat ccgcccaagc acgacgatca
      961 gcgaaaggcc gtccagggcg tcgtggcgcc gcgcaacctg gccaagatgg aagcgctcat
     1021 ccgcggacgc gtcgccgaca ttctcgattc gctgccgctg ggtgagacct tcaactgggt
     1081 cgaccgggtt tccatcgagc tcaccaccca gatgctggcc acgctgttcg acttcccctt
     1141 cgaggagcgg cgcaagctca cctactggtc ggacatggcc acgtccagcg agctggccgg
     1201 cggcccaacg cccgagccag tgcggcgtga ggcgctgatg gaatgtctgg aagccttcac
     1261 ccgaatctgg cacgagcgcg caagccagcc gccggacgag agcgtggatc tgatctccat
     1321 gctcgcccac agcgacgaca cgaaggacat gctcaatcgg ccgatggaat acctcggcaa
     1381 cctgatcctg ctgatcgtgg gcggcaacga caccacgcgc aactcgataa ccggcggcgt
     1441 gctcgcgctg aaccagaatc ccggcgagta cgacaagctg cgccagcatc ccgagctcat
     1501 tcccaacatg gtggcggaga tcatccgctg gcagacgccg ctggcctaca tgcgccggac
     1561 ggccacccag gacacggagc tcggcggcaa gctgatccgc aagggcgaca agatcgccat
     1621 gtggtacgtc tccggaaacc gcgacgagac ggccatcgac cagcccgagg agttcatcat
     1681 cgatcggccc aacgcccgcc atcacctttc cttcggcttc ggcattcacc gctgcatggg
     1741 caaccgcctg gcggaaatgc agctgcgcat cgtctgggag gagatcctca aacgcttcca
     1801 cacggttgag gtggtgggcg aaccggtgcg cgttcagtcc agcttcgtca agggatactc
     1861 gcagctgccc gtgcgactgc atccggtcta gtttgcgcgc ggcccctcgg aacggcgggt
     1921 gtcagggggc tcagcctggg gtgcccgttg cggttatccg ccgtcgagca gcaccgtgcg
     1981 gatgtcgttg gcgacggtat ccgcatgaag gaaagagatg ctgtagatgt tgcgtatctg
     2041 cctgtcccgg tcgatgaggt agacgcgcag aatgtgcgac agcgtgccaa cgaactcccc
     2101 gtcggcgtct atgtcgcgca ccacccactg atcgtacgca tccagcaccg aggtgagatc
     2161 cgctgcgttc gcaaccgtga cgaagcgcca gtcgaacccc ggcttgcgga agtggccggc
     2221 gtactgtcgc aggaccgccg gcgtgtcgtg ggacggatcg aagctgaagc ttacgagccg
     2281 caccttaccc tggaggtcag gggcgctggt gatggccccc tgcagcttgc cgagcacgtg
     2341 ggtcgccagg ggacagccgt tgacgtcgtt gcaacgggtg tagatgaagc tcagcacgac
     2401 caccttgtcg ccgagcattt cgtgcagccg tccgataact ccctgggcat cgaccaccct
     2461 ggcatcggcc acttccccca ggggcggcag cgcgtagccg cccacggggg gcggggcgaa
     2521 gtccagatcg gcgtatcccg gcgcaagcac caccggcgcg gcatgctggt gcgaagcgcc
     2581 ggagtcaggc tgcgcgtgag ctccaccggc ggtgaaagcc accgccagcg tgctcaacag
     2641 gcaggcacgc catcccaccg gtcaatgcag tcccacctgc tgcggcgggc gtttgccgta
     2701 gagcgcgtat gagccgaagc gcatctggtg cggcgcgccc agcccggccg ccacgaaatc
     2761 aatcgtgaac cccggcgtca tcttctcgcc atcccagccg tagagcttga agtactgcag
     2821 gtccggaccg ctgggtcccg tcttgtccca gttggccagc agcgaggagg tgaagtagag
     2881 ccgctcgccg tcccagctct gggacaccat gttgacctgc tctccgatct tctgctcgaa
     2941 cagctgcttc ggcgcgtggg gatcggagat gtcgtagagg tgcaccagac cgtcattcca
     3001 ggtattgacc cagaggaggt tgtcttccgc agagatcgaa atgtccaccg gcagcgggat
     3061 cttggcagca tcgccgatat ccgctacggc cttggcctgc cactcgccgc cctcgtcctc
     3121 gtagatcagc cagatcttgg aggtcaacgc cgtggaggtg aagcagtagt tgcttttggc
     3181 gccccaggcg cagcgaatct ccagcggggc gccgggaacg tcaagaatct tctttggctg
     3241 acgggcatgc agatcccaca cgacgaccgt gttgccgaag cgcttcatcg cctccgcatc
     3301 ggccatcatg gcgcctaagt tcatcatgta gttgttccac cccgtgaacg aggacgtgat
     3361 catcacgttg cgccggggca gcacccgaac gtcgtagccg taaccatcgg cgtagctgcc
     3421 ggacttgacg gcgccctgca ggttgtcgtc cgtggggatc cagtgagtgg cgatgtaatc
     3481 ccccgcgttg gtgtactcga ccagcgccgt gcggccgccg tggtcacgat tgttagacag
     3541 gccggtgagc atcatccggc ccggcagcgc gtagttggtg tgcgggccta ccacccctcc
     3601 gcttctggcc acgaaatcgt cgatcatccg gtgcagcgtg ggggcgcccg ggttggtgtg
     3661 cacgtcgaag atgaagatct tgctggtatc cagaccgctc gcccagagaa agcgccggtc
     3721 atcggtcagg cccgagtgat gcgcctcgtt gcggccgccc accgacaggc tgtgcaccac
     3781 ctctccatag gttttcgagg tcgggttgac gtccacggtc acgagcttgt cctgaccgtc
     3841 gcccacgccc tccatcccga gcgtccagac gtaaacgaaa tcttcctgcc cgacgatctt
     3901 ggccatgtag ggtgacatgc acgtctcgtc tgcccagacg ggcatggcga gcaacgcgcc
     3961 cgccgccagc tgcgcgagca tcttcctgat agtcttcatc ggttccccct gttctggaac
     4021 ggtctcgcag gtatcctatc accccaatgg agtcggtcct aagacacatg tggcgaggcc
     4081 cgatcaccag tcaggcagta gccgccctca tgctgaccgc gtgctgcacc tgggccgtca
     4141 cgcccggtga tccgagagac gggtacgagc acgcccacta cgagacggca tcgaggcaac
     4201 tcagcgcact ccgcggatcg gcgctggcgc tcgcggagct ggcaggcaac ccgcctctcg
     4261 gcttgccgcc cctgccggca gagcggttgc cggtggcggc ggccatcgac ctcggccgac
     4321 ggctgttctt cgacaggcga ctgtctgcca acgcgacgct gtcctgcgcc atgtgtcacg
     4381 tgccggagca gggtttcacc cagaacgagc tgaaaacccc ggtgggcatc gaaggccgct
     4441 tcgtgcaccg caacgcaccc tcgctctaca acgtggcgta ccagcaagcc ctgtttcacg
     4501 acggccggga agacgacctg gccaaccaga tctgggcccc gctgctggca gccaacgaga
     4561 tgggcaacgc ctcccgcgag gccgtggtcg agcgcctggg gggcctggag gagtacgagg
     4621 gcgagtttct ccgggtgttc ggcgacggga tcagcgccgc aacactgggt cgggcgctgg
     4681 ccagctacca gacggctctt ctgtcggccg actccccctt cgaccgctgg tacttcggcg
     4741 gcgaggccga tgccgtcggc gcggaggtca aacgcggatt tcgatcattc gtcaatgaag
     4801 gttgcgcgac ctgccatcag ctgcagacgg gccacgccac gttcacggac ggggcctacc
     4861 acgataccgg catcggcttc ctggcagcgg ccagtgcggg cacgccggcc cgccgcctgc
     4921 agatcgcgcc cggggtcatc atcgacgtcg aaacggacat cgcgaccccg cacgtcgagg
     4981 acgacggccg agcagccgtg acgggggatc cggccgaccg ctggcgatac cgaacgccgg
     5041 gtttgcgcaa cgtggcgctg agcgcgcctt acatgcacga cggctcgctc gccaccctgg
     5101 aggcggtcat cgactactac gcagcagggg gcgctcccca cgacggccag gacgcccgaa
     5161 tcaggccgct gtctctgggc gctgatgaac gcgccgacct cgtcgccttc ctgcaggcgc
     5221 tgacaggcag caacgtcgac gccctggctg gcgacgccag ggccgcgccc atcggcgaca
     5281 cgcatcacgg tccttgagcg cggtctgagt gctcagctcc cggcgcggac gacggagaag
     5341 cggaacgcct tgccatcgat ctcggagtcc gccgcctggg atcccggatc gccctcggca
     5401 aagtcggtgg ccagagtctc cgcagcgatg tagtcacggt gttcggcggc cgcctcgacc
     5461 agagcccgat ccccagcgaa gcagacctcg atgcgatccg ccacgttcag ctccagctcc
     5521 ttgcgccggc gctgaatgcg gttgacgatc tcgcgggcgt aaccgccgcg cacgagctcg
     5581 tcgtccagaa cgcagtccag atcaacggcg atacggcggt ttgagatggt gttggtccca
     5641 ggtcgcgctt tctgcagcac ctcgatctcc tcgaggttga agacctcccc gtcgagctcg
     5701 atctcgccct cggcctgaaa ggcggcgatc tgctcgggag acaaggtcgc aatcgcgctc
     5761 tgaaaggcct tcatccgctt gcccagccgc ttgccgagca cgggaaagtt cggcttggcg
     5821 accacctcga tgtacgcgcc ttcgtcgtca tcgtagctca ccgccaggac gttgagctcc
     5881 gttttgacat agctctccag ccgggcgagc tcctcgagca gcgctcggtc gcggttgacg
     5941 atggtcaggc gtctgagcgg cgtgcgaaga ccgatcttca cctcctcgcg cttctgacga
     6001 ccgagcagca cgacctgctg catgcggtcg acggcttctt ccagctcgct cctgatcagc
     6061 gcgccctccg gctccggata atcgcacaga tgcaccgact cgggcttggc ccggccgccg
     6121 cccagcgccg acagctcctg gtacacgtgc tccgcaagga acggcgcgaa aggcgccatc
     6181 acctggcaga gctcgtacag cgcggcgtag agcgtgctgt aggcggcaat cttgtctgcc
     6241 gtgatgtcct cgccccagaa ccgctgacgg ttgaggcgga tgtaccagtt ggtcaggtcc
     6301 tcgatgaaat cgaacagctg cgggaccacg ttgtagagct tatagacctc catctcccgg
     6361 ccgacgtttg ccttgagggt ctgcaggcgc gagaggatcc agtgatccag gacgttgtcg
     6421 ccggtgtgct gacccttgtc cggcgaccac tgatcgatct ccgcgtaggt cttcaggaaa
     6481 gtgaacgcgt tgtaccaggg cagcagggcc cggcgcgtca tgtcccgcac gccggcatcg
     6541 gcgaaacgct gctcctcccc ccgcatcagc cccgagttga tgagatacag gcgcagggcg
     6601 tccgcgccgt aagtttccat gagctcatcg ggcggggtgt agttgcgaag ccgcttggac
     6661 atcttcttgc cgtcctccgc catcaccatc ccgttgacga tgacgttgcg gaaagcgggc
     6721 ttgtcgtaga gcgcggcggc tagaaccgtc agcgtgtaga accagcctcg cgtctggtcc
     6781 agcccttcgg cgatgaactc ggccggaaag ccggccttga agaccttctc gttctcgaac
     6841 ggatagtgca gctgggcata gggcatggag ccagactcga accagcagtc cagcacctcc
     6901 tcaatccgat ggtaggt
//
LOCUS       DBNHOX010000732         4135 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_59144, whole genome shotgun sequence.
ACCESSION   DBNHOX010000732 DBNHOX010000000
VERSION     DBNHOX010000732.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_59144"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..630
                     /locus_tag="AC16UZ_15920"
     CDS             187..630
                     /locus_tag="AC16UZ_15920"
                     /inference="COORDINATES: protein motif:HMM:NF025691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HIR6248225.1"
                     /translation="MEEVMKQRLTSRNLWRRVLYMLLFVLAYGIAEAVVAVVVVVQFF
                     FILISGRANENLLVFGNNLSTYVRQILQFQTFNSEYLAYPFGDWPDEGLGSELWLEEG
                     TAEEAEAAPPVAGSEPPDEAEPDSGVAERGPIEGSADPEEPDPRG"
     gene            complement(647..979)
                     /locus_tag="AC16UZ_15925"
     CDS             complement(647..979)
                     /locus_tag="AC16UZ_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248226.1"
                     /translation="MNQRVVAGILALALCLHALPASARLYSYVNEEGDYVVSQKKPKN
                     VDEYAVLTDEGEFIRLVKRKRDDVPITHWRPWYMPNEPDPINPVEPGLEQRTPEVLIE
                     EVDGPEPN"
     gene            complement(1034..>4135)
                     /locus_tag="AC16UZ_15930"
     CDS             complement(1034..>4135)
                     /locus_tag="AC16UZ_15930"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6248227.1"
                     /translation="GRCTVNVELFENADDNKVLGDVKNRVDAIETFPQEAEKPIITLV
                     AAERTVVEIAVTGPSDERELKSIAQQVRDDLAALPGITRVDVSGVRPYEISIEISEAT
                     LRRYGLSFDEVANAVRAASLDLPGGAIKTESGEVLLRTEGQAYRGSEFEDLVILTRGD
                     GTRVFLRDLATVVDGFEDTDERVWFDNKPAALVQVFRVGDQDILAVTRAVKSYLDDAD
                     ERLPQGVDLTIWNDNSEFMRDRLDTLMNSARQGFMLVLLMLALFLRPRLAFWVSVGVP
                     AAFLGAIFVANLAGLSIDGISTFGFILVLGILVDDAVVIGERVHSLQSRAGESPPSET
                     LISAAIEGTRQVSMPVIFGVLTTITAFLPLTFGPGPTGQIQSVISVIVLCCLTFSLIE
                     SQLVLPAHLGHRRVQTAAGEVGLMMVPLVAIVLMEFAWDLRSFFAIGIGTAAVLIAWS
                     IQGGFERFAARVIALQKQFSDRLEHVIQFRFRRLVSAATDARYVTAAGAVAILGITVG
                     LVAGGHMPFTYFPSLPADRISAKLTMPLGTPARVTEEIVTNIAVTAEVLQRELASEQP
                     DQPPVRFILAAVGAHPVSSAGLGAVIAPPAARTGGHMGEVTLQLTPSDERDLSTLEIA
                     NRWRDATGPIPDAVEFTFATSTFSVGSEIDIQLAGPDLEELANVAAEIRSELAAYPGV
                     IDITDSFRSGKRELKLDILPAGEALGLNLGALARQVRQAFYGEEIQRIQRGRDDVRVM
                     LRYTEAERRALGSLDEMRIRTRNGSEVPFATVASADLGRGFSAIKRTDGRRVVNVTAD
                     VDRNVITANEVLADFGPGPLRTILRDHPRVSFSLEGTRREQEDSIASLLPMFLMALAV
                     IYALLAVPLRSYTQPLIIMSVIPFAMAGAVWGHMIMKSLGLLSNIVIMSVTGGIAACG
                     VVVNSSLVLVHGVNARRREGMDIHSAVVEAAVTRCRPILLTSLTTFAGLSPLLLNQSV
                     QAQILIPMATSVAWGVVFATVVTLLVVPVGYLILDDLGRKLSRGRTSVSPSSADAGAS
                     AGR"
BASE COUNT          744 a         1310 c         1363 g          718 t
ORIGIN      
        1 tgtggtgaat ctcgccatct tcgaggtggg cctgtgaggg caaccatctg aaaatcatcg
       61 gaaaaaagaa ttggtaccaa tcttgcgaca ctgcctggca acagagggcc ccagcggccc
      121 gggcgaactc gccccggtgt ccgtcgaacc ctcagccgtg atgtgttggc aaatcaagga
      181 ggcgccatgg aagaagtgat gaagcaacgg ctcacgagcc gaaacctctg gaggcgcgtc
      241 ctttacatgc tgctcttcgt gcttgcctac ggcattgccg aggcggtcgt tgcggtggtg
      301 gtggtcgtgc agttcttctt catcctgatc tcgggtcggg ccaacgagaa cctgctcgtt
      361 ttcgggaaca atctcagcac ctatgtccgc cagatcctgc agtttcagac cttcaacagc
      421 gagtatctgg cttatccgtt cggggactgg ccggacgagg gtctgggtag cgagctctgg
      481 ctcgaggagg gcacggccga ggaggcggag gccgcgcccc cggtggccgg gtcagagcct
      541 ccggacgagg cggagcccga ctccggcgtg gccgaacgcg gtccgatcga gggatcggct
      601 gatccggagg agcccgaccc gagaggctga gcgcgatggg tctcggctag ttgggctcgg
      661 gcccgtcgac ctcctcgatg agcacctccg gggtacgttg ctccagcccg ggctccacgg
      721 gattgatggg atccggctcg tttggcatgt accacgggcg ccagtgggtg atggggacgt
      781 cgtcgcgctt gcgcttgacc agacgaatga actcgccctc atcggtaagg acggcgtact
      841 cgtccacgtt cttcggcttt ttctgcgaaa ccacgtagtc gccctcctcg ttgacgtacg
      901 agtagaggcg ggccgatgcg ggtagcgcgt gcaggcagag tgcgagcgcg aggatgccgg
      961 caacgacccg ctggttcatc tggtcgatcc gctggctgtt ttgctggagc ggcgtcattg
     1021 tctgcatatc acctcaccgc ccggcggagg ctccggcgtc ggcgctgctt ggcgaaacgg
     1081 acgtccggcc ccggctgagc ttcctgccga gatcatcgag tataaggtaa ccgacgggca
     1141 ccaccagcag ggtgaccacc gtggcgaaga cgacgcccca ggctacggag gtggccatgg
     1201 ggatgaggat ctgcgcctgc acgctctggt tgagcagcag gggtgacagg ccggcaaacg
     1261 tagtgagtga cgtcagcagg attggccggc agcgcgttac cgctgcctcg accaccgcgc
     1321 tgtggatgtc catgccctct cgacgccgcg cgtttacacc gtgcaccagc acgaggctcg
     1381 agttgacgac gaccccgcag gctgcgatgc cacccgtcac cgacatgatc acgatgttgg
     1441 acagcagccc aagcgacttc atgatcatgt gcccccacac ggccccggcc atggcaaagg
     1501 ggatcaccga catgatgatc agcggctgcg tgtacgagcg cagcggcacg gccagcagag
     1561 cgtaaattac cgccagcgcc atcaggaaca tggggagcag gctggcgata ctgtcctcct
     1621 gctcccggcg tgtgccttcc aggctgaagc tgaccctggg gtggtcgcgc agaatggtcc
     1681 tcagcggtcc tggaccgaaa tcggccagca cctcgttcgc cgtgatcacg tttcgatcca
     1741 cgtcggcggt cacgttcacc acgcggcgac cgtcggttcg cttgatggcg gagaagccgc
     1801 ggccgagatc ggcgctcgca accgtggcga agggaacctc ggagccgttg cgggtgcgga
     1861 tgcgcatctc gtcaagggac ccgagagcgc ggcgctccgc ctcggtgtag cgcagcatca
     1921 cccggacgtc gtcgcggccg cgctgaatcc gctgaatttc ttcgccgtag aacgcctgtc
     1981 ggacctggcg agccagggcg cccaggttca gcccgagcgc ttctcccgcc gggaggatat
     2041 cgagcttcag ctcccgtttt ccggagcgga aagagtcggt gatatcgatg acgcccgggt
     2101 aggcggccag ctcgctgcga atctcggccg ccacgttcgc caactcttcc agatcgggcc
     2161 cggcgagttg gatgtcgatc tcggagccta cgctgaaagt ggaggtcgcg aaggtaaact
     2221 ccacggcatc cgggatgggg ccggttgcgt cccgccatcg gttcgcgatc tccagggtgc
     2281 tgaggtcgcg ttcgtcgctg ggcgtaagct gcagcgttac ctcccccatg tgccctcccg
     2341 tcctggcggc cggaggcgcg atgacggcgc ccaggccggc ggagctcacg gggtgcgcgc
     2401 ccacggccgc caggatgaag cgcacgggcg gctgatcggg ctgttcgctg gcgagctctc
     2461 gctgcagcac ctccgccgtg acggcgatgt tggtgacgat ttcctcggtg acccgggccg
     2521 gcgttcccag aggcatcgtg agcttcgcgc tgatccggtc cgccggcagc gagggaaaat
     2581 aagtgaacgg catgtgtccg ccggcgacca ggccaaccgt tatcccgaga atggccaccg
     2641 cgccggccgc ggtgacgtag cgggcgtccg tggccgcgct gaccaggcgc cggaagcgga
     2701 actggatgac gtgctcgagg cggtcggaaa attgcttctg cagggcgatc acccgagcgg
     2761 caaagcgctc gaagcctcct tggatgctcc aggcgatcag caccgccgcc gtgccaatgc
     2821 cgatggcaaa aaagctgcgc aggtcccagg cgaactccat cagcacgatg gccaccagcg
     2881 gaaccatcat caggcccacc tcgcccgcgg cggtctgcac gcggcggtga cccagatgcg
     2941 cgggcagtac cagctgggat tcgatcagcg agaacgtcag gcaacaaagc acgatcacgc
     3001 tgatcaccga ctggatctgc cctgtgggcc ccggcccgaa ggtgagcggt aagaacgccg
     3061 tgatcgtggt cagcaccccg aagatgacgg gcatggatac ctgtcgtgtg ccctcgatgg
     3121 ccgcgctgat gagcgtttca ctcgggggtg actcacccgc ccgggactgc agagagtgca
     3181 cgcgctcgcc gatcaccacc gcgtcatcca ccagaatgcc cagcacgagt atgaagccga
     3241 aggtggaaat gccgtcgatg gacaaaccgg ccagattggc gacgaagatt gcgcccagaa
     3301 aggccgcggg tacgcccacg ctgacccaga aagccaggcg tggacgtagg aacaaggcga
     3361 gcatcaggag caccagcatg aatccctgcc gcgcgctgtt catcagggta tccagacgat
     3421 ctcgcatgaa ctcggaattg tcgttccaga tcgtcagatc cacgccctgt ggcagacgtt
     3481 cgtcggcatc atccaggtag gatttcacgg ctctggtcac tgccagaatg tcctggtcgc
     3541 ccactcggaa aacctgcacg agcgcggcgg gcttgttgtc gaaccagacc cgctcgtcgg
     3601 tgtcctcgaa gccgtcgacc actgtggcca ggtcccgcag gaacacacgg gtaccatccc
     3661 cgcgcgtgag tatcaccagg tcctcgaact ctgagccccg gtatgcctga ccctcggtgc
     3721 gcagcaggac ttcgccgctt tcggtcttga tggcgccgcc tggaaggtcc agagaggcgg
     3781 cgcgcacggc gttggcgacc tcgtcgaagc tcaaaccgta gcggcgcagg gtcgcctcgg
     3841 aaatttcgat ggagatctcg tagggccgca cgccagagac gtccacccgg gtgattcccg
     3901 gaagagctgc cagatcgtcg cgcacctgtt gagcaatgga cttgagctcc cgctcgtcgc
     3961 tcgggccggt cacggcaatc tcgaccacgg ttctctctgc cgcgaccagc gtgatgatgg
     4021 gtttctcagc ctcctggggg aacgtctcga tggcgtcgac ccgattcttg acgtcgccga
     4081 gaaccttgtt gtcatcggcg ttctcgaaca gctccacgtt caccgtgcag cggcc
//
LOCUS       DBNHOX010000733         1797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_766201, whole genome shotgun sequence.
ACCESSION   DBNHOX010000733 DBNHOX010000000
VERSION     DBNHOX010000733.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_766201"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /gene="rsgA"
                     /locus_tag="AC16UZ_15935"
     CDS             <1..622
                     /gene="rsgA"
                     /locus_tag="AC16UZ_15935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR6248228.1"
                     /translation="TKADLCDDLERYVDGARRIVPGLEVVALDARDRAQLAPLASWLA
                     TGRTVALLGSSGVGKSTLVNTLSEAQVQRTAQIREDDGKGRHTTSHRSLHPVPGGGLM
                     LDSPGMRELGMADAELGVRQMFEDVEALAHQCRFADCAHDSEPGCAVKAALEAGELTE
                     ERLESYRKLSREEAVNAETIAERHARSRATDKLHRRVQDASPKRRS"
     gene            683..>1797
                     /locus_tag="AC16UZ_15940"
     CDS             683..>1797
                     /locus_tag="AC16UZ_15940"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6248229.1"
                     /translation="MADLLAEQLRLQPVSVTRRMSASLPGLRRLAQEKGLKIHHLGAG
                     YPHPEVTDPRSFIAHREAYFAHLEEREGLNDPRALPEYLRESFSYTDTLGPAGTRAAF
                     ARVYGRDWGVTMDPGRLIPTVGATGGINLACSMFERPGVPLAYITDAPTYAGFNARAT
                     LCQHAAIYSVPMDDEGPLPDVLCDQIRAARADGRFVPFYYTVPDGHNPAGFSFSQARR
                     EAILRVVREEGILILEDAPYVYINFAPAANRPRPFLAMDPRCTIHLFTGSKIGFPGPR
                     VGFLYSEAELAIAGGRTASLTEFALTEASADMLFQNPAALLGFEALLHDDDFSERRSL
                     WPVAETKLAVYRENRSIMLDGLERELGGHRDLFHWTDP"
BASE COUNT          286 a          623 c          593 g          295 t
ORIGIN      
        1 caccaaggcg gatctatgcg acgatctgga gcgctacgtg gatggggccc gacggatcgt
       61 cccgggcctc gaggtggtgg ctctggacgc tcgcgaccgg gcacagctcg ctccccttgc
      121 gtcctggttg gcgaccggcc gcacggtggc gctgctcggc tcgtcgggtg tgggtaaatc
      181 cacgctggtc aacaccctct ccgaggctca ggtgcagcgc acggcgcaga tccgggaaga
      241 cgacgggaag ggcaggcaca ccacctcgca ccgctcgctg cacccggtgc ccggtggcgg
      301 gctgatgctg gacagcccgg gtatgcggga gctcggcatg gcggatgcgg aactcggcgt
      361 gcgtcagatg ttcgaggacg tggaagcgct cgcgcaccag tgccgctttg ccgactgcgc
      421 gcacgactcg gaacccggct gcgcggtcaa ggcggccctc gaggccgggg agcttaccga
      481 ggagcggctg gagagttacc gcaagctcag ccgggaagaa gccgtgaacg cggaaaccat
      541 tgccgaacgt cacgcccgct cccgagcgac ggacaagctg caccggcggg tccaggacgc
      601 atctcccaag cgccgcagct gacgcctacc tcgggagccg gagatgcctt aaagtacgcg
      661 cctcttccgc ccgaggcgcg tcatggccga ccttctcgcc gagcagctcc gcctgcagcc
      721 cgtttccgtc acccgtcgca tgtctgcctc tttgcccggc ctgcgcaggc tcgctcagga
      781 gaaggggttg aagatccacc acctgggagc ggggtatccc catcctgaag tgaccgatcc
      841 ccggtcgttc attgcccatc gggaagccta cttcgcccac ctcgaagagc gggaaggcct
      901 caacgaccct cgagccctgc ccgagtacct gcgggagtcg ttctcctaca cggatacgct
      961 gggcccggcc ggcacgcggg cggcgtttgc ccgcgtttac ggccgggact ggggagtcac
     1021 gatggatccc gggcgcctca taccgacggt gggggcgacc ggcggcatca atctcgcctg
     1081 ctccatgttc gagcgcccgg gcgtgccgct ggcttacatc accgacgccc cgacctacgc
     1141 cggattcaac gcccgtgcaa cgctgtgtca gcacgccgcc atctacagcg tgcccatgga
     1201 tgacgaaggc ccgctgccgg acgttctctg tgatcagatc cgcgccgcgc gggccgacgg
     1261 ccgcttcgtg ccgttctact acacggtgcc ggacgggcac aacccggcag ggttctcgtt
     1321 cagccaggca cggcgggaag ccattctccg ggtcgtcagg gaggagggca tcctcattct
     1381 cgaggacgct ccttacgtct acatcaactt tgcgccggcc gcgaaccgcc cccggccctt
     1441 cctcgccatg gacccgcgct gcacgatcca cctcttcacc ggatccaaga tcggctttcc
     1501 cgggccgcgc gtaggctttc tctactcgga agccgagctt gccattgccg gcgggcggac
     1561 ggcttcgctg accgaatttg cgctgactga agccagcgcc gacatgctgt ttcagaaccc
     1621 ggccgccctg ctgggcttcg aagcgctgct gcacgacgac gacttcagcg agcggcggtc
     1681 gttgtggccg gtggcggaga cgaagctcgc cgtgtatcgc gagaaccggt cgatcatgct
     1741 cgacggcctg gaacgggagc tcggcgggca tcgggatctg tttcactgga ccgaccc
//
LOCUS       DBNHOX010000734         1703 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_894809, whole genome shotgun sequence.
ACCESSION   DBNHOX010000734 DBNHOX010000000
VERSION     DBNHOX010000734.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_894809"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="AC16UZ_15945"
     CDS             complement(<1..514)
                     /locus_tag="AC16UZ_15945"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6248230.1"
                     /translation="MAGILTLLAACDPAGTEPTGTDTDAAPKPVTVGPAPSAIGDRRL
                     LWGDTHLHTSYSPDAYLMQNRSADPDTAYRYAKGLPVVHPYHKARIQIGTPLDFLVVS
                     DHGEFMGVVPMVLQGDPRVANTETGKRYRAMAEAGREIEVFGELIAQVNKIVPPDQDL
                     YNPDINRSVWG"
     gene            complement(574..>1703)
                     /locus_tag="AC16UZ_15950"
     CDS             complement(574..>1703)
                     /locus_tag="AC16UZ_15950"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6248231.1"
                     /translation="GQPLDFLVITDHSDAMGAMNEVVAGNPDLLRDPTVRDWHQRISK
                     GGESAFEAALEVVDSFTKGNTPDVLMSGTFAERIWNGVTETADAFNEPGRFTAMIGYE
                     WTSTTDGNNLHRNVLYRDGADSAKKVLPFTVIDSDNPEDLWTWMENYEATTGGEVLAT
                     AHNGNLSNGLMFPDVNPATNRRLTESYAARRARWEPIYEVTQIKGDGEAHPLLSPNDE
                     FADYETWDQGNLGPNLKDDDMLQYEYAREALKKGLAHEVRLGVNPYKFGLQGATDSHT
                     GLATAEEDNFFGKHSQTEPTAARLATDVGHFENNVWPGWYQVASGYTGVWATENTREA
                     IFDAMMRKEVYAAKYFGTTAPEESLMTTQERAYTSPIWYTP"
BASE COUNT          246 a          529 c          577 g          351 t
ORIGIN      
        1 cgccccaaac cgagcggttg atgtccgggt tgtagaggtc ctggtccggt gggacgatct
       61 tgttgacctg ggcgatgagc tcgccgaaca cctcgatctc gcggcccgcc tcggccattg
      121 cccgatagcg cttgccggtc tccgtgttgg cgacccgggg atctccctgc aggaccatgg
      181 gcaccacgcc catgaactcg ccgtgatcgg aaaccaccag aaaatccagc ggcgtgccga
      241 tctggatgcg cgccttgtga taggggtgca ccacgggcag ccccttggcg tagcggtagg
      301 cggtgtccgg atccgccgag cggttctgca tcaggtacgc gtccggtgag taggacgtgt
      361 gcaggtgggt gtccccccag agtagccttc tgtctccgat tgccgacggg gccgggccca
      421 cggtgacggg tttgggcgcg gcgtcggtgt ccgtgcccgt gggctcggta ccggccgggt
      481 cgcaggcggc cagcagagtg agaatgccgg ccaagcaagt gcccccaatc atccttgttt
      541 tcatggtccg cttccttcga gttactttcc cggtcagggg gtgtaccaga tcggtgaggt
      601 atatgcacgt tcctgggtgg tcatgaggga ttcctcggga gctgtggtgc cgaaatactt
      661 ggcggcgtag acctccttgc gcatcatggc gtcgaatatc gcctcccggg tgttctccgt
      721 ggcccacact cccgtgtagc ccgacgccac ctgataccag ccgggccaga cgttgttctc
      781 gaaatggccg acgtcggtgg ccagtctcgc ggcggtgggc tcggtctgcg agtgcttgcc
      841 gaagaagttg tcctcctcgg cggtggcgag gccggtgtgg gagtccgtcg ccccctgcag
      901 gccgaacttg taggggttga cgccgagccg gacttcatga gccagcccct tcttgagcgc
      961 ctcccgggca tactcgtact gcagcatgtc gtcgtccttg agattggggc ccaggttgcc
     1021 ctggtcccag gtctcgtaat cggcgaactc gtcgttgggc gagagcagcg ggtgcgcctc
     1081 accgtccccc ttgatctggg tgacctcgta gatcggctcc cagcgcgcgc ggcgcgcggc
     1141 gtatgactcg gtgagccggc ggttggtggc cgggttcacg tccgggaaca tcagcccgtt
     1201 gctcaggttg ccgttgtggg cggtggcgag aacctcaccg ccggtggtgg cctcgtaatt
     1261 ctccatccac gtccacaggt cttccgggtt gtcgctgtcg atcacggtga agggaaggac
     1321 cttcttggcc gaatcggcgc cgtcccggta gagcacgttg cggtgcaggt tgttgccgtc
     1381 cgtggtggac gtccactcgt agccgatcat ggcggtgaat cggccgggct cgttgaaggc
     1441 gtctgcggtt tccgtcaccc cgttccagat gcgctctgcg aacgtgccgg acatgaggac
     1501 gtcgggggtg ttgcccttgg tgaacgagtc gacgacctcc agcgccgcct caaaagccga
     1561 ttcgccgccc ttagagatgc gctgatgcca gtcccgcacg gtggggtccc tcagcagatc
     1621 cggattgccc gccaccacct cgttcatggc tcccatggca tccgagtgat cggtgatcac
     1681 caggaagtcc agcggttggc cga
//
LOCUS       DBNHOX010000735         8845 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_445246, whole genome shotgun sequence.
ACCESSION   DBNHOX010000735 DBNHOX010000000
VERSION     DBNHOX010000735.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8845)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8845)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8845
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_445246"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="AC16UZ_15955"
     CDS             complement(<1..518)
                     /locus_tag="AC16UZ_15955"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6248232.1"
                     /translation="MAEAIPTREIQSWPEQALRGHRIPGSRYTSKEFAEKEWEGMWTK
                     VWLLLGREEELPNPGDWQCEEVGPESILMVRQKDGGVKAFYNVCQHRGNPLVDGEKGS
                     VRRFVCKYHSWAFMPDGELNFAPDADDFPEGNPCGKIRLEELACETFAGFVWVNMDTE
                     CAPLKDFLGPIW"
     gene            626..1267
                     /locus_tag="AC16UZ_15960"
     CDS             626..1267
                     /locus_tag="AC16UZ_15960"
                     /inference="COORDINATES: protein motif:HMM:NF018717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR6248233.1"
                     /translation="MSRPAYMIIAIDVRDPSRMAEYAGGTQPLLEAAGARVLAATNGI
                     TVEDGVWARERIALLEFPSMESARSFWSADAYAPLKALRESISTSDVVLVEGTQDERV
                     EEEDGAHYLLGASTMLNADWVEEYIQKVPPISARFGVKMLAGGPEFETLEGAWPGESV
                     VVLRFPSEHAFREFWYGDDYRPMKELREANAPGHHISFAETFELPDAGGPSQG"
     gene            1341..3977
                     /locus_tag="AC16UZ_15965"
     CDS             1341..3977
                     /locus_tag="AC16UZ_15965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR6248234.1"
                     /translation="MSPIAPLVVTPTARLARTLSRRVALERAAGGAGAWVPPRIQSFP
                     AWLANQREDYFLTATDHRVPIGAHQALWLWQSLIDRQVFIGDPEVAALAQRAWRLIHE
                     HALEAPERWPELMLSEDARAFRGWAAAYRTECDRRGVVDEWAFAGELPGLIEAGHLAI
                     PQGLELAGFDLPMTPLQVRIFDALEAMGCAVSGRPEGSASRTSQTAAMELIRFHLPDD
                     ELRGAAGWARSVLEAEPGRTVAVVVPDLAGRLERVESIFREVFDPPASVLGPRGAEPF
                     HVSLGRRLSEWSLVADALAFLELGSQRLTQPQAGRLLRSPFLAGWPDETLDRSRALAR
                     LVSRAPFEVTVNELRWALDGVGAAALDEALQSWQTLREAAAQSAWPSDWAGRFQQELS
                     ALGFASGRPLDSREYQVLNRWHDLLESFGALDVVAAGPWSRTQALRQLAERARSSVFR
                     ERNPGVPVEVLGVEEALGSSFDAVWITTLDSATWPGSPVREPLIPAAVQASVPRASGD
                     GCLARAQLELDALCASAPDVRGSYARGVEDQALEITGLLRHLPVIDAPPPTPAPGARF
                     AEPFEDHEAPKRPAGPVSGGTGVLRDQSDCPFRAFARWRLGAVDLTPPRPGLDAAQRG
                     TVIHRALERFWEDLDGQAALQGLDRDAREHRIAAAAAAAVDDLVSRHRAILGPGARRL
                     EERRVARALTRWLGLEVQRAGFEVVDREGEVAMTFAGLELEGKIDRLDRLPDGRLMLI
                     DYKTGRTSRNAWLPEARIVDPQLPAYAVSMPSAPAAITFARIRADDSRFDGIADGDPG
                     TAGVIPLADAGPRYGELGSWQALLSGWRGLLDALAQDFRSGRAAVDPRDADACKTCHL
                     HALCRIRDRDPYRDGEGGSEDD"
     gene            3970..7299
                     /locus_tag="AC16UZ_15970"
     CDS             3970..7299
                     /locus_tag="AC16UZ_15970"
                     /EC_number="3.1.11.5"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR6248235.1"
                     /translation="MTDRRARAEAVEPDRSVIVQAPAGSGKTTLLVERFLGLLGTVEA
                     PEEVLAITFTRKAAAEMRERVLRFLDPDFQSDEPHEQGPLNRAREVRDRVAAWRLLEN
                     PQRLLIRTIDSFNYYLARTMPVASALGPVPAPADDTASLYREAARGVLSLVGTSDGVA
                     PALELLLDWRDHQSQGVEDLLAGLLAQREQWLRAAGIAGDAERGVLEAALEEVVRGRL
                     ERADAALNAALSAAGFSSAELAGLLRAAARTLRDENRESTTLVFLEADGLPRPDPERI
                     DHWNALVAALLTANLSKPAFRRSVNVSNGFQRQSEEKAEFEALLGAFGENAGLLEALH
                     RARGLPAPRYDDESWQVLDALVQVLKRAAVELETLFARRGRTDYAGLAAAALRGLGDE
                     DGVTDLGLYLDRRIRHVLVDEFQDTNFSQLHLLEKLTAGWEPGDGRSLFLVGDPMQSI
                     YRFREAEVGLFIRVRDQGLGPLSLESKRLASNFRSREEIVRWVNDHLGPIFPEVEDAA
                     AGAVAYAPSDGERGAGGAVEVLARSERSAEAEAVADLVSRALDDHRDDAEYRAAIIVR
                     ARSHLAEILPALVRRGVPYRAVKLDPLGNRPVVQDLLALTRVILQPADVAALLAVLRS
                     PVCGLTLADLHALAGDGRSPLEADALARLAGEPRRRAARVFDVLGDARLAWRRFSVRA
                     LVDGAWRRLGGPECCPDEAARRDAAVYLDALDAAERGGLLEDWNDFRESLAGRHAEGD
                     PQSEGVKLEVLTMHGAKGLEWDLVVLPALDRAPGRPASTMLHWLPFLEADGREEVLLA
                     PLRAAEVAQDPPLVKLIRAEQQVRERYENQRVLYVACTRARERLVLSATIDPDKDPFT
                     PPSGSLLADLWATAGAEFEAALDTSGGAAAGPTVPDQRLNRIAADWEPPLGDRVAWKP
                     ELETRERAAPVEYNWAGVQARRIGTVVHRLLERVGQVGAEDFTDAHREHLLTRIPVLL
                     QSMGTRPDDLDRSAAVVADALARTLDSETGRWLLSGSHEEGACELPIAGMIDGRLVNA
                     VVDRTFVDADGVRWIVDYKSGYHAGSDLEDFFAQERERYQEQLDGYRRLFEQLEDRPV
                     KTALYLPRHGRLEVVAPAGYP"
     gene            complement(7325..7972)
                     /locus_tag="AC16UZ_15975"
     CDS             complement(7325..7972)
                     /locus_tag="AC16UZ_15975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR6248236.1"
                     /translation="MGKISLTIDNGPHPTVTPEVLDALADRQVTATFFLIGQEAARPG
                     GLDLIEHISKAGHPIGNHTWSHTVAFGLNPGASAIADEVARTQKLIAPFASQPPMFRP
                     AGGGGRLDSSLLSAELIDYLCRERYTCALWNVVPRDWEDPQGWVNRALREVGTLDWSV
                     VVVHDVIDGNADQIAAFIDRARDDGHEFVTDIAPECLPIVGGAITADLAALTGSR"
     gene            8117..>8845
                     /locus_tag="AC16UZ_15980"
     CDS             8117..>8845
                     /locus_tag="AC16UZ_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen
                     decarboxylase/cobalamine-independent methonine synthase
                     family protein"
                     /protein_id="HIR6248237.1"
                     /translation="MSADRILTTHTGSLPRPDDLIQMMWAKGDGIPVDEGALAERVAA
                     AVQETVDKQVAAGISIVNDGEMSKPSYATYVKDRLSGFGGESPERYYFADLDAYPRSA
                     EIVAANPGRRKRSAPACNAPIGVQDAEAAALDMVNLCRSAEGADVDGIFSSAASPGVV
                     SLFFGNEYYASDEEYVFAIAEAMRHEYEAIAAAGATVQLDCPDLAMGRHTSYADMDLT
                     AFRKRVAMNIEALNHGVRNIPPEQL"
BASE COUNT         1290 a         2917 c         3114 g         1524 t
ORIGIN      
        1 tcccagatgg ggcccaggaa gtccttcagc ggcgcgcact ccgtgtccat gttcacccac
       61 acgaacccgg caaacgtctc gcaggcgagc tcctccaaac ggatcttccc gcacgggttg
      121 ccctcgggaa agtcgtccgc gtccggcgcg aagttcagct ccccgtcggg catgaacgcc
      181 cagctgtggt acttgcacac gaagcgcctg acgctgccct tctcgccgtc caccaacggg
      241 ttgccgcggt gctggcagac gttgtagaag gccttcacgc cgccgtcctt ctggcgcacc
      301 atcaggatgg actcggggcc cacctcctcg cactgccagt cgccggggtt cggcagctcc
      361 tcttcccggc ccagcagcag ccagaccttg gtccacatgc cttcccactc tttctcggca
      421 aactccttcg aggtgtagcg ggatcctggt atccggtgcc cgcgcagcgc ctgctcgggc
      481 cagctttgaa tttcgcgggt tggaattgct tcggccattg tgctttctct cgctctgacg
      541 ttttgttcgc tccgggctgc cgagtataac gcgttcactt tcccttatga tgtgcaactt
      601 aaagagcgcg tcagggagtc agcccatgag ccgaccggca tacatgatta tagccattga
      661 tgtcagggat ccgtcacgaa tggccgagta cgcgggcggc acacagccgc ttctggaagc
      721 cgccggcgcc agggtgctcg ccgcgaccaa cgggatcact gtcgaagacg gtgtctgggc
      781 tcgggagcga atcgccctcc tcgagttccc ctcgatggag agcgccaggt ccttctggag
      841 cgctgatgcg tacgcgccgc tgaaggcgct gcgagaatcc atcagcacgt cggacgtggt
      901 cctggtcgag ggtacgcagg acgagcgggt tgaagaggag gacggtgccc actacctgct
      961 cggggcgagc accatgctga acgcagactg ggtggaagag tacattcaga aggtgcctcc
     1021 catctcggcc cgctttggcg tcaagatgct ggccggaggc ccggaattcg agaccctcga
     1081 aggtgcctgg cccggcgagt ccgtggttgt gttgcggttc ccttccgagc acgccttccg
     1141 ggagttctgg tacggtgatg actaccgccc gatgaaggag cttcgcgaag ccaacgcccc
     1201 ggggcatcac atctcttttg cggaaacctt cgagctgccc gacgccggcg ggccatccca
     1261 aggttgaggc ggtctccccg cccttggccg ggtactgtcc gatcgagccg ttcggctcgc
     1321 cttgatcggg tgaggcacgg gtgagcccga ttgcgcccct cgttgtgacg cccaccgcca
     1381 ggctcgcccg aacgctttcc cgtcgcgtgg ctctggagcg ggcggccggc ggggccggtg
     1441 cctgggtgcc gccgcgcatt cagagcttcc cggcctggct ggccaatcag cgcgaggatt
     1501 acttcctcac cgccaccgat catcgcgttc ccatcggcgc ccatcaggcg ctgtggctgt
     1561 ggcagtcgct catcgatcgc caagtgttca tcggcgaccc cgaggtggcg gcgctggcgc
     1621 agcgggcatg gcggctcatc cacgagcacg ccctggaagc gccggagcgc tggcccgagc
     1681 tcatgctgtc ggaggacgca cgggcgttcc gcggctgggc tgccgcgtac cgtaccgagt
     1741 gtgaccggcg cggtgtggtg gacgaatggg cgttcgccgg ggagctgccg ggactcatcg
     1801 aggcgggcca cttggcgatc ccgcagggac tggagctggc cggcttcgat ctgccgatga
     1861 cgccgctgca ggtccgcatc ttcgatgcgc tcgaggctat gggctgcgcg gtctccggac
     1921 gccccgaagg gtctgcgtcc cgaacttccc agacggcggc aatggagctc atccgctttc
     1981 atcttcctga cgacgagctg cgcggcgcgg cgggctgggc gcgatctgtg ctggaagcgg
     2041 agccggggcg aacggtcgcc gtggtggtcc cggatctggc gggacggctc gagcgggtgg
     2101 agtccatttt ccgggaggtg ttcgacccgc cggcatcagt ccttggtccg cgaggtgccg
     2161 agccgtttca cgtttccctg ggcagacgcc tctcggaatg gtccctggtt gctgacgccc
     2221 tggcctttct ggagctgggc agccagcggc tcacccagcc tcaggcgggc cgattgctgc
     2281 gctctccctt tctcgccggc tggcccgacg agacgctcga tcggtcccgg gcgctggcgc
     2341 gcctggtttc ccgggcaccc ttcgaggtga ccgtcaacga gctgcgctgg gcgctcgacg
     2401 gggtcggtgc ggcagccctc gatgaggcgc tccagagctg gcaaacgctg cgcgaggcgg
     2461 ctgctcagtc ggcatggccg tcggattggg cgggccgatt tcagcaggag ctgtcggctc
     2521 tcgggttcgc ctcggggcgg cccctggaca gccgcgagta tcaggtgctc aatcgctggc
     2581 acgatctgct ggagagtttc ggcgctctgg acgtggtggc cgccgggccc tggtcccgta
     2641 cgcaagccct gcgacagctt gcagagcgtg cccgatccag cgtctttcgt gagcgcaacc
     2701 ccggcgtgcc cgtggaggtt ctcggcgtcg aggaggcgct cggctccagc ttcgatgcgg
     2761 tttggatcac caccttggac agcgcgacgt ggccgggttc gccggttcgc gaaccgctga
     2821 taccggctgc cgttcaggca tccgtgccgc gggcgagcgg agacggatgc ctggccaggg
     2881 cccagctgga gctggacgcg ctttgcgcgt cggcccccga tgtccgcggc agctatgccc
     2941 ggggcgtaga ggatcaggcg ctggagatca ccggcctgct ccgccatttg ccggtcattg
     3001 acgcgccccc gccgacgccg gcgcccgggg cacgttttgc agagccgttc gaggaccatg
     3061 aggctccgaa gcgcccggcc ggtcccgtca gcggcggaac cggcgtgctg cgtgaccagt
     3121 ccgactgccc gttccgggca ttcgcgcgct ggcgactcgg tgcggtggat ctcaccccgc
     3181 cgcgcccggg gctcgacgcg gcccagcgag gcaccgtgat tcatcgtgcc ctcgagcgct
     3241 tctgggaaga cctggatggc caggccgcac tgcaggggct ggatcgcgac gcccgcgagc
     3301 atcgcatcgc ggccgcggcc gcggccgccg tggacgatct cgtgagccgg caccgcgcca
     3361 ttctcggccc gggcgctcgc cgtctcgagg agcgccgcgt cgctcgggca cttacccggt
     3421 ggctggggct ggaggttcag cgagccggtt tcgaggtggt ggatcgggaa ggcgaggtgg
     3481 cgatgacgtt cgcgggcctg gagctggagg gcaagatcga tcgccttgat cggctgcccg
     3541 acgggcggct gatgctcatc gactacaaaa cgggccgcac gtcccgcaat gcctggttgc
     3601 cggaagcgcg catcgtggac ccccagctgc cggcctatgc ggtgtccatg ccctcggcgc
     3661 cggccgccat cacttttgcg cgtatccgcg cggacgattc gcgcttcgat gggattgccg
     3721 acggtgatcc gggtaccgcc ggggtgatcc cgctggcgga tgccggtccc cgctacgggg
     3781 agctgggctc ctggcaggcg ctcctgtccg gctggcgcgg cctgctcgat gcgctggccc
     3841 aggacttcag gtcgggtcgc gctgccgtgg atccgagaga cgccgacgcc tgcaagacct
     3901 gccacctcca cgcgctgtgc cgcatccggg accgggatcc ttaccgcgac ggggaagggg
     3961 gctcggaaga tgactgatcg aagagcacgc gccgaggccg tggagccgga tcgctccgtc
     4021 atcgtccagg ctccggccgg atcgggcaag accacgctgc tggtcgagcg ttttctaggc
     4081 ctgctgggaa cggtagaggc gccggaagag gtgctggcca ttacctttac ccgcaaggcg
     4141 gcagcggaga tgcgtgaacg tgtgcttcgc ttcctggacc ccgacttcca atccgatgag
     4201 cctcacgagc agggtccgtt gaaccgcgcc cgcgaggttc gtgaccgggt ggcggcctgg
     4261 cggcttctgg agaatccgca acgtctgctc atccgcacca tcgacagctt caactactac
     4321 ctggcccgaa ccatgccggt ggccagcgcc ctgggtcctg tcccggcgcc cgccgacgac
     4381 acggcgtcgc tttaccgcga ggccgcacgc ggcgtgctga gcctggtcgg aacctccgac
     4441 ggggtcgctc ccgccctgga gcttctgctg gactggcgcg atcaccagag tcagggcgtg
     4501 gaggaccttc ttgccggcct gctagcccag cgggagcagt ggttgcgggc ggcgggcatt
     4561 gccggcgatg cggagcgcgg cgtgctggaa gccgccctgg aggaggttgt gcggggcagg
     4621 ctcgaacggg ccgatgcggc cctgaacgcg gcgctctccg cggccggttt ctcctctgct
     4681 gagcttgccg gcctgctgcg ggcggcggcc cgcacgctgc gcgacgagaa ccgtgaatcc
     4741 accacccttg tcttcctgga agccgacggg ctgccccggc ccgatcctga gcgcatcgat
     4801 cactggaacg cgctggtggc cgccttgctc accgcgaact tgagcaagcc ggcctttcgg
     4861 cgcagcgtga acgtgagcaa cgggtttcag cgtcagagcg aggagaaggc ggaattcgaa
     4921 gcgctcctcg gcgcgttcgg ggagaacgcc gggttgctcg aggccctcca ccgggcccgc
     4981 ggactgccgg cgccgcgcta cgacgacgag agctggcagg tgctcgacgc gctcgttcag
     5041 gttctcaaac gtgccgccgt agagctggag acgctgtttg cccggcgcgg gcgaactgat
     5101 tacgcggggc tcgctgctgc cgctctgcgg gggcttggcg acgaggacgg cgtgaccgac
     5161 ctcggcctct atctggaccg gcgcatcagg cacgttctcg ttgacgagtt tcaggacacc
     5221 aacttcagcc agttgcacct gctggagaag ctcacggcag gctgggagcc gggcgacggc
     5281 cgcagcctct tcctggtggg cgacccgatg cagtcgatct atcgctttcg ggaggcggag
     5341 gtgggtctgt tcatccgtgt ccgggaccag ggccttgggc cactttcgct ggagtcgaag
     5401 cggttggcga gcaacttccg ctcccgggag gagatcgtcc gctgggtcaa cgaccacctg
     5461 ggtccgatct ttccggaggt ggaggatgcc gctgctggag ccgtggccta tgcgccttcc
     5521 gacggggagc ggggcgctgg cggtgccgtg gaggttcttg cccgatccga acggtcggcc
     5581 gaagccgagg ccgtggccga tctggtcagt cgcgccctgg acgaccatcg agacgatgcg
     5641 gaataccggg cggccatcat cgttcgggcc cgcagccatc tggcagaaat cctgccggcg
     5701 ctggttcgcc gcggggtgcc ctaccgcgcg gtgaagctgg accccctcgg caaccggccc
     5761 gtcgtgcagg atctgctggc cctgaccagg gtgatcctcc agcctgcgga cgtcgccgct
     5821 ctgctcgcgg tgctgcgttc gcccgtatgc gggctgaccc ttgccgatct gcacgcgctc
     5881 gccggtgacg gccgctcgcc gctggaggcc gatgcccttg cacgcctggc gggagagccc
     5941 cgacgacggg ctgcgcgggt cttcgacgtg ctgggcgacg cgcgcctggc ctggcggcgc
     6001 ttctccgttc gtgcgcttgt cgacggggcc tggcgccggc tcggtgggcc cgaatgttgc
     6061 cccgacgagg cggcgcggcg tgatgccgcc gtgtatctgg atgcgttgga tgccgccgag
     6121 cggggcggtc ttctggagga ctggaacgac ttcagagaaa gcctcgccgg gcggcacgcc
     6181 gagggcgacc cgcaaagcga gggggtcaag ctggaggttc tcaccatgca cggggccaaa
     6241 ggcctggagt gggatctggt ggtcctgccg gcactggatc gggcccccgg ccgtccagca
     6301 tcgaccatgc ttcactggct gccgttcctc gaggccgatg gtcgggagga agttctgctg
     6361 gcccctctgc gcgcagcgga ggttgctcaa gacccgcctt tggtgaagct gatccgtgcg
     6421 gagcagcagg tgcgggagcg ctacgagaac cagcgtgtgc tctacgttgc gtgcacgcgg
     6481 gcccgggagc ggctggtgct ctccgccacc atcgatccgg acaaggaccc tttcacgccg
     6541 ccctcaggca gtctgctcgc cgacctctgg gccacagccg gcgcggagtt cgaggccgcc
     6601 ctcgacacca gcggtggcgc tgccgccggg cccacggttc cggatcagag gctgaatcgg
     6661 atcgccgcag actgggagcc gccgcttggc gatcgggtgg cctggaagcc ggagctggag
     6721 acccgggaac gagccgctcc ggtggaatac aactgggccg gtgtgcaggc gcgccggatc
     6781 gggacggtgg tgcaccggtt gctggagcgg gtgggtcagg tgggtgccga ggacttcact
     6841 gatgcccacc gcgagcatct gctgacccgc atacccgtcc tcctgcagtc catgggaacg
     6901 cgacccgacg atctggatcg gtctgcggcg gtggttgccg acgcgctggc acgaaccctt
     6961 gacagcgaga ccgggcgctg gctcttgagc ggcagccacg aggaaggcgc gtgtgagctt
     7021 cccatcgcgg gcatgatcga cgggcgactg gtcaacgccg tcgtcgaccg caccttcgtg
     7081 gacgcggatg gcgtgcgctg gatcgtggac tacaaatccg gctaccacgc cggttcggat
     7141 ctggaggact ttttcgccca ggagcgcgag cgctatcagg aacagctcga cggctaccgg
     7201 cggcttttcg agcagctcga ggaccgcccc gtgaaaacgg cgctgtacct gccgcgtcac
     7261 gggcggttgg aggtggtggc tccagccggt tacccgtaaa cgccgcggcc cgcaggagat
     7321 ggttctaacg tgagccggtc aaggccgcca ggtcggcggt gatggcccct cccacgatgg
     7381 gcaggcactc gggcgcgatg tccgtgacga actcgtggcc gtcatcccgg gcccggtcga
     7441 tgaaggcggc aatctgatcg gcgttcccgt cgatgacgtc gtgaacgacc accacgctcc
     7501 agtccagcgt gccgacctct cgaagcgccc ggtttaccca gccctgcggg tcttcccagt
     7561 ctcggggcac cacgttccac aacgcgcagg tgtagcgctc gcggcagagg tagtcgatga
     7621 gctccgctga gaggaggctc gagtctagcc gcccgccgcc accggcggga cggaacatgg
     7681 gtggctggct cgcaaagggc gcgatcagct tctgcgttcg ggcgacctcg tcggcgatgg
     7741 ccgacgcgcc cgggttcagg ccgaacgcaa cggtgtggct ccaggtgtga ttgccgatcg
     7801 gatggcccgc cttgctgatg tgctcgatga gatccagccc gccgggtcgt gccgcctcct
     7861 ggccaatcag aaagaacgtg gcggtgacct gcctgtcggc gagcgcgtcg agcacttcgg
     7921 gagtgactgt cggatgcggt ccgttgtcga ttgtcaggga gatcttgccc aaggatttgg
     7981 tcctggccgg ttgggtcgct cgagagtcgg gggatcttag cagaaggcgc ttgccgagtt
     8041 tcggcggcca ccggctgcgg ctgggagccg ctttgcagat gagctaatct tcgggcggaa
     8101 ggccacgggg aagcacatga gcgcagaccg aattctcact acccacacgg gcagcctgcc
     8161 gcgcccggat gatctcattc agatgatgtg ggcaaaaggg gacggcatcc cggtagacga
     8221 gggggcgctt gccgagcggg tcgccgcggc cgtccaggag accgtggaca aacaggtggc
     8281 ggcgggtatc agcatcgtca acgacggcga gatgtcgaaa cccagctacg ccacctacgt
     8341 caaggaccga ctcagcgggt tcggcggtga gtctccggag cgctactact tcgccgacct
     8401 cgatgcgtat ccccggagcg cggagatcgt tgccgccaat cccggccggc gcaagcgctc
     8461 ggccccggcc tgcaacgcgc cgatcggcgt gcaggacgcc gaagcagccg ctctggacat
     8521 ggtcaacctg tgccggtcgg ctgaaggcgc cgacgtggac gggatcttct ccagcgccgc
     8581 ctcgccgggt gtggtctccc tgttcttcgg taacgagtac tacgcctccg atgaggagta
     8641 cgtgttcgcc atcgccgagg ccatgcgcca cgagtacgag gccattgccg ccgccggcgc
     8701 cacggtccag ctcgactgcc ccgatctcgc catgggccgg cacacctcgt atgcggacat
     8761 ggacctcacc gctttccgca agcgggtggc catgaacatc gaagcgctca atcacggcgt
     8821 cagaaacatc ccgcccgagc agctc
//
LOCUS       DBNHOX010000736         2116 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_445259, whole genome shotgun sequence.
ACCESSION   DBNHOX010000736 DBNHOX010000000
VERSION     DBNHOX010000736.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_445259"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /gene="cofE"
                     /locus_tag="AC16UZ_15985"
     CDS             complement(<1..747)
                     /gene="cofE"
                     /locus_tag="AC16UZ_15985"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HIR6248238.1"
                     /translation="MDKPEATSRTASPGFSVLGIPGVPMVSPGDDLTAIIVDALRDAR
                     ITLEDGDIVCVAQKIVSKAEGRQIPLASVTPSEEARTLAEETDKDPRLVELILRESSE
                     VMRKKEGVLIVRHRLGLVGANAGIDQSNISHEDGEQALLLPEDPDRSAAELKSALDEA
                     SGARVGVIITDSANRPWRLGTVGIAIGCAGISVLDDHRGGVDLFDRELKVTLISRADG
                     IAGAATLVMGETTERIPVALVRGFPAEQSTD"
     gene            complement(758..1417)
                     /gene="npdG"
                     /locus_tag="AC16UZ_15990"
     CDS             complement(758..1417)
                     /gene="npdG"
                     /locus_tag="AC16UZ_15990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006740 - NADPH regeneration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR6248239.1"
                     /translation="MAETIAILGGTGDLGTGLAIRWSKAGHKIIIGSRTLEKAQNAVG
                     NLHKISPETPADAMVNADAAAAGDIVVLTVPAEHQLSTLEGVKQSLTGKILIDVTVPL
                     VPPKVGTVQLPPEGSAGKRAQDMLGEDVMVVTAFQNIAAHLLQEDVHIECDVLVAGNK
                     KAARDKVIELVEQAGMKGYHAGPIENSAAAEALTSILIQINRRHDISHSGIKIVGQSD
                     H"
     gene            complement(1498..1965)
                     /locus_tag="AC16UZ_15995"
     CDS             complement(1498..1965)
                     /locus_tag="AC16UZ_15995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6248240.1"
                     /translation="MASFTDLSEKFTEITERIVWCTVSTVDAKGRPRSRILHPVWEGA
                     TGWIATGRHSHKARHLANNPYVSLSYWDPQHEQTMIECKAEWQDDQPTRDRIWELLKT
                     TPEPVGYDPALFWSSAADENFGVLKLTPWRLEVWSLAAMSAGKPAQVWRQEVG"
BASE COUNT          307 a          700 c          694 g          415 t
ORIGIN      
        1 gtcggtggat tgctcggccg ggaagccgcg caccagggcc acgggaattc tctccgtggt
       61 ttcgcccatg accagggtgg cggcgccggc gatgccgtcc gcccggctga tgagggtgac
      121 cttcagctcc cggtcgaaca gatcgacgcc gccgcgatga tcgtcgagca cgctgatgcc
      181 cgcgcagccg atggctatgc ccacggtgcc cagccgccag gggcgattgg cgctgtcggt
      241 gatgatcacg ccgacccggg cgccgctggc ctcgtcgagg gcagacttca gctcggccgc
      301 cgagcggtcc gggtcctcgg gaaggaggag cgcctgctcg ccatcctcgt gggagatgtt
      361 cgactggtcg atccccgcgt ttgctccgac caggccaagg cgatggcgca cgatcagcac
      421 gccctctttt ttccgcatca cctctgacga ttcgcgcagg atcagctcga cgagccgcgg
      481 gtccttgtcc gtttcctccg ccagcgtccg ggcctcctcg ctcggcgtca ccgacgccag
      541 aggtatctgg cgtccctcgg ccttggagac gatcttctgc gcaacgcaga cgatgtcgcc
      601 gtcttccagg gtgatgcggg catcgcgcag ggcgtcgacg atgatggccg tcaggtcgtc
      661 cccggggctc accatgggca cgcccgggat gcccagcacg gaaaagccgg gcgatgccgt
      721 cctgctggtg gcttccggct tgtccattcg cgtttggtca gtgatccgat tgaccgacga
      781 tcttgatccc ggagtgggag atgtcgtggc gcctgttgat ctgaatcagg atcgaggtga
      841 gcgcttcggc cgcggcggaa ttctcgatgg gccccgcatg gtagcccttc atgccggcct
      901 gctcgaccag ctcgatgacc ttgtcccggg cggccttctt gttccccgcc accagcacgt
      961 cgcactcgat gtgcacgtct tcctgaagga ggtgggcggc gatgttctga aaggcggtaa
     1021 cgaccatcac gtcctcaccc agcatgtcct gggcccgctt gccggcgctg ccctcgggcg
     1081 gaagctgcac ggtgccgacc ttcgggggaa ccaggggcac ggtcacgtcg atgagtatct
     1141 tcccggtgag cgattgcttg acgccctcca gcgtcgacag ctgatgctcg gcgggaacgg
     1201 tcaggacgac gatgtcccca gccgcggcgg catcggcgtt caccatggcg tcggctggcg
     1261 tttccgggct gatcttgtgg aggttgccca ccgcgttctg cgccttctcc agcgtccgcg
     1321 agccgatgat gatcttgtgg cctgctttgg accaccgtat ggccagcccg gtgccgagat
     1381 ctccggtacc gccaagtatg gctatggtct ctgccatgtc tttctcccgc ttgttgctca
     1441 ttgccggcgc gcccgggtgc aggcgctgac ggcggtgaaa agcgggcgat tatgccgcta
     1501 accgacttcc tggcgccata cctgcgccgg cttgccggcg gacatggcgg ccagggacca
     1561 gacctcgagc cgccagggtg tgagcttgag gacaccgaaa ttctcgtccg cagcgcttga
     1621 ccagaacagc gcggggtcgt agcccacggg ctccggcgtg gtcttcagca gttcccagat
     1681 ccggtcccgg gtgggctggt cgtcctgcca ctcggctttg cattcgatca tcgtctgctc
     1741 gtgctgagga tcccagtagc tcagcgagac gtaggggttg ttcgcgaggt gccgggcctt
     1801 gtgcgagtgg cggcccgtgg caatccagcc cgtggcgccc tcccagaccg gatggaggat
     1861 gcgcgagcgg ggccggccct tggcgtccac cgtgctgacc gtgcaccaca cgatgcgctc
     1921 cgtgatctct gtgaacttct cgctgagatc agtaaatgac gccacgtcca ctcgccggcc
     1981 tccccgttcg cctccaggcg gctttgttgc cggccctggt ttgccttatt gtgcggcatt
     2041 tcccgacctg tacaaccatc gcctaggggg cagaaatgaa aataggactc atgatgggcg
     2101 ccaccggcac caccac
//
LOCUS       DBNHOX010000737         6146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_894926, whole genome shotgun sequence.
ACCESSION   DBNHOX010000737 DBNHOX010000000
VERSION     DBNHOX010000737.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_894926"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="AC16UZ_16000"
     CDS             complement(<1..226)
                     /locus_tag="AC16UZ_16000"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6248241.1"
                     /translation="MTTIAELLELEEIKNLRHMYSHYFDGKQVDKLADLFTDDAICEF
                     GPDYGGDWVGKEQIRANYATYTEAEGDEHEV"
     gene            376..1203
                     /locus_tag="AC16UZ_16005"
     CDS             376..1203
                     /locus_tag="AC16UZ_16005"
                     /inference="COORDINATES: protein motif:HMM:NF018038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR6248242.1"
                     /translation="MEPVRVLLVTKGHAFDHDAFLAMFEGMDELDVTMVEQPAAQVIL
                     RPENVGDYGAVLFYDMSGIPGIGLMHDGAGDTGIPPDDYRRSIEALIDGGTGIVMLNH
                     GTVSWPLWPLWRSISGSSFMLTEGELNGQRVPGSGYRGGHGPYPNPTISLTPVDPSHP
                     VAAGLGNGFTVTDEIYLKTATLGPKVTPLFESDYEFVAANFTPPPLAPPEEQAAWSHP
                     EGSNVVVWANAAGNSPVVAADIGDSPEAFGNADFRRLLRNALQWVASPEARQWAANR"
     gene            complement(1262..2293)
                     /locus_tag="AC16UZ_16010"
     CDS             complement(1262..2293)
                     /locus_tag="AC16UZ_16010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6248243.1"
                     /translation="MAEHASNEGVNLQLHLKRVPEGLAGPGDFEVRETEIPRPGDGEV
                     LVRAHYFGLDAALRLIVRDSKDFLFRVEPGDLVRGSMAGEVIESNNPDYSVGDLVLAS
                     SGVQNYAISDGSDLERCDTSQAPLGSWLGGFGVSGLTAYFAITEECKPQPGQTVVVNG
                     AAGAVGSIAGQIARLAGARTIGITSSDEKCRWLTEELKYDHAINYKAGNLYDQLAEAA
                     PERIDVIFDNVGGDILDESLRLIGMRGVVLLCGSTSQYAAEHMTGPDNYIWLGTMRAR
                     MQGFVVFDYADRYEEARRRMARWIKDGQLKLPEYVVDGDIGDYPEAFLQLYQGANRGK
                     MLLRLPAAR"
     gene            2374..2607
                     /locus_tag="AC16UZ_16015"
     CDS             2374..2607
                     /locus_tag="AC16UZ_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248244.1"
                     /translation="MTLADWLIVLGLGGIWHGVQIIWVGGLPRQVRRGETPTAPKGTP
                     EAFGLFWIDQYGYIGLTLALGGAAAVVAGLLGS"
     gene            2604..2987
                     /locus_tag="AC16UZ_16020"
     CDS             2604..2987
                     /locus_tag="AC16UZ_16020"
                     /inference="COORDINATES: protein motif:HMM:NF013304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIR6248245.1"
                     /translation="MMPVTLLYAGVMGLMSVFLANLVLRARVRPGQPEWRGDATLRVQ
                     ANFVENVPLAVVLMYLVELSGVGAVAMHAFGVSLVALRVLHVWGLDRAPGANVNYPRL
                     IGAQGTFVLLSVLGVTAVYQFLRAT"
     gene            2997..4550
                     /locus_tag="AC16UZ_16025"
     CDS             2997..4550
                     /locus_tag="AC16UZ_16025"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6248246.1"
                     /translation="MNIGNITAKWAALDPSRPAIVDIPTGRRVSFGELDERVRQLANG
                     FCAQGFEKGARIGVLARNSIEYFEVYYACARAGLIAQPLNWRLGVDELARILEDGGPS
                     AIVTSAEFRGERDALQGLVDVPRWLEFGASSDGSFEDLVRASSAEEPQRSDSTGDDDP
                     VLILYTGGTTGLSKGALHSHRSLFMGMVNQTVAEREVPTDVYMLTGQMFHIPVGLAMN
                     YMAHGCPVVLMNFEARQALEIIQAERVSAFLGITTMLNWMMAVDDFASYDLSSLRNIQ
                     YGGGPMPGTVVKAALESFPCTLIQGYGQTEGMTMTFLSQEDHADAVAGKHPERLNSCG
                     REGFVTRVQVVNEQGEPVPKDGATAGEIVVRSDANMLGYWGRPDLTAQTIRNGWMWTG
                     DVATWDEDGYVFIVDRAKDMIISGGENIYSTQVEAAIHKHPAVLEAAVIGVPDDEWGE
                     AVKAVVVLKPGETATEQEIIDTARENLASYQKPKSVDFVDSLPKAPTGKILKRELRDP
                     YWKDQDRNV"
     gene            complement(4547..5728)
                     /locus_tag="AC16UZ_16030"
     CDS             complement(4547..5728)
                     /locus_tag="AC16UZ_16030"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6248247.1"
                     /translation="MSGPLSGIRIIELATMIAVPGATHLLATQGAEVIKVEDPVRGDE
                     LRHYGSEKGGMSAWFINANSGKRSIALDLQHEDGKSVLWRLLETADVVMQGFRPGVIG
                     RLGFDSETVRDRLPAIVYCSSSGFGPHGPYAQLPVYDPVIQALSGWAGAQRYDEGPRL
                     VRGMVADKVAALTSAQAVTAALLQRSMTGRGLHVELSMLEANIAFNWSDVMMHASLLD
                     ADATHRPNLLSSYRLFPCRDGWVTVTAGTDAQWRAFSQALERPELAEDARFVTASARA
                     GHVADWYEAMGEAVAAFRLQEVLDRLRTADVPVAPVLDPTEVAEDPQVLAAGLLREVD
                     HPVAGRLRQPRPSAEIFGSSQDLIPAPTHGQQTAQVLAEIGYDEGAIGRLAADGVVKV
                     D"
     gene            complement(5754..>6146)
                     /locus_tag="AC16UZ_16035"
     CDS             complement(5754..>6146)
                     /locus_tag="AC16UZ_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248248.1"
                     /translation="METWAHGWEIYDCLRLERMHTDRIKNIAAIGVRTFGWTFVNRGL
                     ELPGDPPFVRLTAPSGAIWEWSDPNADSSVTGSAVEFCQVVTQVRNVADTKLEVTGKP
                     AREWMAIAQCFAGAPEDPPAPGSRRPAG"
BASE COUNT         1038 a         1932 c         2047 g         1129 t
ORIGIN      
        1 tgacctcgtg ctcgtccccc tctgcctccg tgtacgttgc gtagttggct cggatctgct
       61 ctttgcctac ccagtctcca ccgtagtcgg gtccaaattc gcagatcgca tcatccgtga
      121 agagatcggc cagcttgtcc acctgtttgc cgtcgaagta gtggctgtac atatggcgca
      181 ggttcttgat ttcctcgagc tcgagcagct ccgcgatcgt cgtcatggtt ccctcccagg
      241 gcctgtctac cttgacgtag attggtgcgt atcgcaggcg ggcgtcaagc cgttgcgtcg
      301 atcccgcgcc ggggttagtt tctggcagcc gcgtccgcag cggggtttcg actaacggga
      361 agagaggacg aatgaatgga gccggtgagg gtacttctgg tcacgaaagg ccacgcgttt
      421 gatcacgatg cttttctggc gatgttcgag gggatggacg aactcgacgt gacgatggtc
      481 gagcagccgg cggcgcaggt catcctgcgc ccggagaacg tcggggacta cggcgccgtg
      541 ctcttctacg acatgtcggg tattcccggc atcggtctca tgcacgacgg ggcaggggac
      601 acgggcattc cgccggacga ctaccgacgc agcatcgagg cactgatcga tggcggaacg
      661 ggcatcgtga tgctcaatca cgggaccgtc tcatggccgc tgtggccctt gtggcgatcc
      721 atatcggggt cttctttcat gctgaccgaa ggtgagctga acggccagcg cgtgccgggg
      781 tccgggtacc ggggcggtca tggtccgtat cccaacccga ccatcagcct gacgcccgtc
      841 gatccttctc atcccgtggc tgcgggcctg ggaaacggct tcaccgtgac ggacgagatt
      901 tacctcaaga cggctacgct tggtccgaag gtgacgccgc tgttcgaaag cgactacgag
      961 ttcgtcgcgg ctaacttcac gccgccgccc ctcgccccgc cagaggagca ggctgcgtgg
     1021 tcgcacccgg aagggagtaa cgtcgtggtc tgggcaaacg cggccggaaa cagcccggtg
     1081 gtggcggccg acatcggtga cagcccggag gctttcggca acgccgattt tcgtcgtctg
     1141 ctgcgcaacg ccctgcagtg ggtggcgtcg ccggaagccc ggcagtgggc ggcgaaccgg
     1201 taggagatca aaaccggatc gtcgcccggg gcatgttcgg gcccggtgct gggggcgaaa
     1261 gtcagcgggc ggccggcagc cgcagaagca ttttgccccg gttcgcgccc tgatagagct
     1321 gcagaaaggc ttccggatag tcgccgatat cgccgtcgac gacgtactcc ggcagcttca
     1381 gctggccatc tttgatccag cgcgccatcc tgcggcgggc ttcctcgtag cggtccgcgt
     1441 agtcgaaaac cacgaatccc tgcattcgcg cccgcatggt acccagccat atgtaattgt
     1501 ccgggccggt catgtgctcg gcggcgtact gagaggtgga gccacacagg agcaccacgc
     1561 cccgcatgcc gatcagccgc aggctctcgt cgaggatatc gccgcccacg ttgtcgaaga
     1621 tcacgtcgat ccgttcgggt gcagcctcgg ccagctggtc gtagaggtta ccggccttgt
     1681 agttgatggc gtgatcgtat ttcagttctt cggttagcca gcggcacttc tcgtcgctgc
     1741 tcgtgatacc gatggtccgc gcgccggcta gccgcgcgat ctgcccggcg atggagccaa
     1801 cggcgccggc cgccccgttc acgaccaccg tctggcctgg ctggggtttg cactcttcgg
     1861 tgatcgcgaa gtaagcggtc aggccggaga cgccaaaccc gccaagccat gatccgagcg
     1921 gcgcctggct cgtgtcgcat ctttccaggt cgctgccgtc gctgatggcg tagttctgga
     1981 cgccggagga cgccaggacc agatcgccga cggagtagtc agggttgttc gactcgatta
     2041 cttctccggc catggagcca cgcacgaggt cacccggctc gacccggaac aggaaatcct
     2101 tgctgtcgcg gacaatgagt cgcagcgcgg catcgagccc gaagtagtga gccctgacca
     2161 gcacctcacc gtccccgggc cggggaattt cggtttcgcg gacttcgaag tcccccggac
     2221 cagcgagacc ctccgggacc cgcttcagat ggagttgaag attgacgcct tcgttgctgg
     2281 catgctccgc catggtgaac cctccccgaa aaactgtatc ctacttcagt tcgggtcggg
     2341 gatgaaaagc ttggaaaagc cgaggggatc gcgatgacgt tggctgattg gttgatcgtc
     2401 ctgggactgg gcggaatctg gcacggcgtg cagatcatct gggtgggcgg gctgcccagg
     2461 caggtgcggc gcggcgagac accgaccgct cccaaaggta cgcccgaggc gttcgggttg
     2521 ttctggatcg atcagtacgg gtacatcggg ctcacgcttg cgctcggtgg cgcggcggcc
     2581 gtcgtggctg gacttctcgg ctcatgatgc ccgtcacgct gctttatgcc ggggtgatgg
     2641 ggctgatgag cgtgtttctc gccaacctgg tgcttcgtgc ccgtgttcga ccgggacagc
     2701 cggagtggcg gggcgacgcc acgcttcgcg tgcaggccaa cttcgtcgag aacgttccgc
     2761 tggctgtggt gctgatgtac ctcgtcgagc tgagcggcgt cggcgcggtc gccatgcacg
     2821 cgtttggcgt ctcgctggtg gctctgcggg ttctgcacgt ctggggcctg gaccgggctc
     2881 ccggtgccaa cgtcaactac ccccggctca ttggagctca gggcactttc gtgctcctgt
     2941 ccgttttggg cgttacggcc gtttatcagt tcctgcgtgc aacctaggag gagtacgtga
     3001 acatcggcaa catcacagcc aagtgggccg cgctggaccc gtctcgccca gcgatcgtcg
     3061 acatccccac ggggcgccgg gtctcgtttg gcgagctcga tgagcgggtg cgccagctcg
     3121 ccaacgggtt ctgtgcccag gggttcgaaa agggcgcgcg aattggcgtg ctggccagga
     3181 acagcatcga gtacttcgag gtttattacg cctgcgcgcg tgcgggattg attgcccagc
     3241 cgctcaactg gcgcctgggg gtggacgagc tggctcgcat cctcgaggac ggcggcccaa
     3301 gcgccatcgt cacgtccgcc gagttccgcg gcgagcggga tgcgctgcag gggctggtgg
     3361 acgtgccgcg gtggctcgag ttcggcgctt cgagcgacgg gagcttcgag gatctcgtgc
     3421 gcgcgtcgag cgccgaggag ccgcagcggt cggactcgac cggggacgac gacccggtat
     3481 tgatcctgta cacgggaggg accacggggc tgtccaaagg agccctgcac tcgcacagat
     3541 cgctcttcat gggcatggtt aaccagacgg tggccgagcg cgaggtgcct accgacgtct
     3601 acatgttgac gggccagatg tttcacatcc cggtggggct ggcgatgaac tacatggccc
     3661 acgggtgccc cgtggtgctg atgaacttcg aggccaggca agcgctggaa atcattcagg
     3721 cagaacgggt gtccgccttt ctcggcatca ccaccatgct caactggatg atggcggtgg
     3781 acgatttcgc gagctacgac ctttcgagcc tccgcaacat acagtacggg ggcgggccga
     3841 tgccgggtac ggtcgtgaag gccgcgctcg agtccttccc ctgcacgctg atacagggat
     3901 acgggcagac agaggggatg accatgacgt tcctttccca ggaggaccat gccgacgcgg
     3961 ttgccggcaa gcaccctgag cgcctcaatt cctgcggtcg cgagggcttc gtgacgcggg
     4021 tccaggtggt gaacgagcag ggcgagccgg tcccgaaaga cggcgccacg gccggggaga
     4081 tcgtggtgcg ctcggacgcc aacatgctcg gctactgggg acgtccggat ctcaccgccc
     4141 agaccatccg caacggatgg atgtggacgg gcgacgtcgc cacctgggac gaggatggat
     4201 acgtcttcat cgtcgaccgc gccaaggaca tgatcatctc cggaggggag aacatttact
     4261 cgacccaggt cgaagcggcc atccacaaac atccggccgt gctggaggcg gcggtgatcg
     4321 gtgttcccga cgacgagtgg ggggaagccg tgaaagcggt ggtggtgctc aaacccggag
     4381 agaccgctac cgagcaggag atcatcgaca cggcccggga gaacctggcc tcttaccaga
     4441 aaccgaaatc cgtcgacttc gtcgacagcc tgccgaaggc tcccacgggc aagattctca
     4501 agcgtgagct gcgggatccc tactggaagg accaggaccg gaacgtctag tcgaccttga
     4561 cgacgccatc ggccgccagc ctgccgatgg cgccctcgtc gtagccgatc tcggccaaca
     4621 cctgtgccgt ttgctgcccg tgggtcggcg cggggatcag gtcctgagag ctcccgaaaa
     4681 tttcggcgct cggtcggggc tggcgcagcc ttcctgcaac cggatgatcg acctcgcgca
     4741 gcagcccggc cgccagcacc tgtggatcct cggcgacttc ggtcggatcg agcacgggtg
     4801 ccacgggcac gtcggccgtt ctcaggcggt cgagcacttc ctggagcctg aaggcggcca
     4861 cggcctcgcc catggcctcg taccagtccg ccacgtgccc ggcacgcgcc gacgcggtga
     4921 cgaaccgggc gtcctccgcc agctccggtc tttccagcgc ctggctgaag gcccgccact
     4981 gggcgtcagt gccagcggtc accgtgaccc atccgtcgcg acaggggaac agccgatagg
     5041 aggaaagcag gttgggcctg tgggtcgcat ccgcatcgag gagggaagcg tgcatcatga
     5101 cgtcggacca gttgaaggcg atgttggcct ccagcatgct gagctcgacg tgtagcccgc
     5161 gacccgtcat cgacctctgc agcagagccg cggtcacggc ctgggcggac gtcagagcgg
     5221 ccaccttgtc ggccaccatg ccgcgcacca ggcgcggccc ctcgtcgtag cgctgtgctc
     5281 cggcccagcc ggacagcgcc tgaatgacgg ggtcatagac cgggagctgg gcgtaggggc
     5341 cgtgggggcc gaagcccgat gacgagcagt agacgatggc aggtaggcgg tctctgaccg
     5401 tttcgctgtc gaagccgagt cttccgatca cgccaggccg aaaaccctgc atcaccacgt
     5461 cggcggtctc caggagtctc cagagcacgc tcttgccgtc ctcatgctga aggtccaggg
     5521 cgatcgagcg cttgccggag ttcgcgttga tgaaccaggc actcatgccg cccttctcgc
     5581 tgccgtagtg gcgcagctcg tcaccgcgca ccgggtcttc caccttgatg acctcggccc
     5641 cctgggttgc aagcaggtgc gtggccccgg ggaccgcgat catcgtcgcc agctcgatga
     5701 tccgtatgcc gctcaagggt cctgacatgc aaacctccga tgctgacgcc gtttcaacct
     5761 gcggggcggc ggctgccggg cgcaggcgga tcctcgggag caccggcaaa gcattgggca
     5821 atggccatcc actccctggc cggcttgccg gttacctcga gtttcgtatc ggcgacgttg
     5881 cgtacctggg tgacgacctg gcagaactcc accgcgctgc cggtgacgct gctgtccgcg
     5941 ttcggatcac tccactccca gatagctccg gaaggggcgg tcaggcgaac gaaaggcgga
     6001 tccccgggca attccaggcc ccgattgaca aaagtccagc cgaaagtgcg cacgccgatg
     6061 gctgcaatgt tcttaatccg gtcggtgtgc atcctttcca gccggagaca atcgtagatt
     6121 tcccacccgt gggcccacgt ctccat
//
LOCUS       DBNHOX010000738         1848 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1472839, whole genome shotgun sequence.
ACCESSION   DBNHOX010000738 DBNHOX010000000
VERSION     DBNHOX010000738.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1472839"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1020)
                     /locus_tag="AC16UZ_16040"
     CDS             complement(121..1020)
                     /locus_tag="AC16UZ_16040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6248249.1"
                     /translation="MIVVTGANGNLGRRLLQSLSRRAPLRAVVRSERAAEQIRALTLE
                     PEPEICVVDYQDAAAMTGALAGAEYVVHLVGIIKETAASTYVDAHERTSEVLCQAADS
                     AGALRIVYLSIVGSEPGSSNPCLASKGAAEAILLAAATPALVVRVPMVLGEGDYAAGA
                     LARRARKGWNVLLRGASLEQPIYAGDVIEAVKAGLGSAPAPAALDDAVLDLAGPVSLS
                     RSDLTRRSASILGRSTRVISLPLFLGLLLAGLLERLLANPPVTAAMLRVLDHDDRLDP
                     EPACHVLGIELTPLDEVLARCLR"
     gene            1164..1691
                     /locus_tag="AC16UZ_16045"
     CDS             1164..1691
                     /locus_tag="AC16UZ_16045"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR6248250.1"
                     /translation="MWFTWANLLTTVRLALAVPCAMAVVSGDWPLAAALFALAVITDL
                     LDGPLARRLNHASPVGGLFDHATDALFVTFALGALATAGYLTWALPCLVAAAFIQYAL
                     DSRALAGKHLRTSYLGRMNGIAYFFAAGIPIVKEGLALPWPSAALIQGLAWVLVVTSI
                     VSMGDRARAWLLRSE"
BASE COUNT          316 a          638 c          604 g          290 t
ORIGIN      
        1 ccactccgag gaccgtctgg ccgactttct cgggtcaccg cgcaaaacta cttgaattcg
       61 cccagcctga atgtgaactg ggtgggaatt cagatggccg tgacgggcgt cacggtttcg
      121 tcatcgcaga caccgggcga ggacctcgtc gagcggcgtg agctcgatgc cgaggacgtg
      181 gcaggcgggc tccggatcca agcggtcgtc atggtcgaga acccgcagca tggcagcggt
      241 gaccggcggg ttcgcgagca ggcgctccag cagaccggca agcagcagcc ccaagaacag
      301 gggcagggat atcacccggg tggatcgacc caggatgctc gcggaccggc gcgtaagatc
      361 gctgcgcgag agggaaacgg gcccggccag gtccagcacg gcatcgtcca gggccgccgg
      421 agcgggggca gaaccgagcc cggccttcac cgcctcgatg acgtctccgg cgtagatggg
      481 ctgctccagg ctcgcgcccc tgagcagaac gttccaaccc tttcgcgcgc gccgcgccag
      541 cgcgccggcg gcgtagtcac cctcgcccag caccatgggc accctcacca ccagcgcggg
      601 cgtggcggcg gccaagagga tggcttccgc agcccccttc gacgccagac acggattgct
      661 gctgcccggt tccgagccga cgatgctcag gtacacgatc cgcaaggcgc ccgccgagtc
      721 ggccgcctgg cagagcacct cggaggtgcg ctcatgagca tcgacgtagg tgctggccgc
      781 cgtctccttg atgataccta cgagatggac gacatattcc gcccccgcga gcgccccggt
      841 catggcggca gcatcctggt aatcgaccac gcatatctca ggttcgggtt ccagcgtcag
      901 agccctgatc tgctcggcgg cccgctccga gcgcaccacg gcccgcagcg gggcccggcg
      961 ggagaggctc tgcagaagcc gcctgccaag attgccgttt gctccggtga cgacgatcat
     1021 gggcagcccc tgccgatcca tggatcggcg ggcgataccg gcaggctctg gtgttcgacg
     1081 cgcatcacgg gccacattct ggagcgcgcg aacgcgctca gcaaggcgac accgatgggt
     1141 ggctggcgta gcataatcgc gccatgtggt tcacctgggc gaatctcctc accaccgtcc
     1201 gcctggcgct tgcggtgccg tgcgccatgg ccgttgtctc cggtgattgg ccgctggccg
     1261 cagccctgtt cgcgcttgcc gtgatcaccg acctgctcga cggcccgctc gcgcgacgct
     1321 tgaaccacgc cagcccggtc gggggcttgt tcgaccacgc gacggacgcg ctcttcgtca
     1381 ccttcgctct gggcgccctg gccactgccg gctacctgac ctgggcgctc ccctgcctgg
     1441 ttgctgcggc tttcatacag tatgccctcg actcccgggc gctggcgggc aaacacctgc
     1501 ggacgagcta tctcgggcga atgaacggca tcgcctactt cttcgccgcc ggcataccca
     1561 tcgttaaaga ggggctggcc ctaccgtggc ccagcgccgc cctgatccag gggctggcct
     1621 gggtgctcgt cgtcaccagc atcgtttcca tgggcgaccg tgcccgagcc tggctgctcc
     1681 gatccgaata gctcccgatt cgcgatcggc aggaacacca ccccgatcaa cgcgttgaac
     1741 agcacgaaga aggtgtgcat cacgaggctg aaagcaccga cctcgggaaa gtccgggaaa
     1801 agaatggtcc agaaaagaag tgcgccggca tggaacggca gcggcagc
//
LOCUS       DBNHOX010000739         6400 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_445465, whole genome shotgun sequence.
ACCESSION   DBNHOX010000739 DBNHOX010000000
VERSION     DBNHOX010000739.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_445465"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="AC16UZ_16050"
     CDS             complement(<1..548)
                     /locus_tag="AC16UZ_16050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6248251.1"
                     /translation="MRGTIARLSALLTAVAILLIGHGLQLTLLPVHAEGLGWSASAIG
                     TSGSAYFLGFVVGCVLIPPVVGRVGHIRTFMVMGALATVALLAAGLLVTLPAWLIFRF
                     VTGFALSGLYMVIESWLVDVTPSNNRGTILALYTVICLLGMSVGQAFLGIASPMSLEL
                     FMAGAALICLAIVPVGLARVAAP"
     gene            complement(557..1255)
                     /locus_tag="AC16UZ_16055"
     CDS             complement(557..1255)
                     /locus_tag="AC16UZ_16055"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6248252.1"
                     /translation="MTARYQVVSQLGAGWESEVYLIRETATGIERTAKVFFPHRNRGN
                     KVARRTAEKLHKLRDCPALIQYVAQETITVRRQAVTVLISEYVEGDLLSEFIARQPGG
                     RMHRFEALHLLRALALALEPIHERREYHGDLHAGNVIVRRVGLDFDVRLIDVYHLDQR
                     KSLSVKDDVCDAIRIFYDALGGQRFYARQSPEIKAICRGLKRSLISERFRTAGQLRRY
                     LDTMSWEFEPRRGR"
     gene            complement(1358..1768)
                     /gene="mce"
                     /locus_tag="AC16UZ_16060"
     CDS             complement(1358..1768)
                     /gene="mce"
                     /locus_tag="AC16UZ_16060"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HIR6248253.1"
                     /translation="MIGKLNHVAIVVPDLESAAALYRDTLGATVSDPVPLPDHGVTTV
                     FVELPNSKIELLHPLGDGSPVANYLERNPKGGMHHLCYEVQDIEAAIAHLVAEGATVL
                     GDGKPSTGAHGLPVVFLHPKDFCGTLIELEEVAA"
     gene            complement(1797..3965)
                     /gene="scpA"
                     /locus_tag="AC16UZ_16065"
     CDS             complement(1797..3965)
                     /gene="scpA"
                     /locus_tag="AC16UZ_16065"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HIR6248254.1"
                     /translation="MAENPTRKSDPAGQDEATWKELAAKELRGKPPDELTWVSPEGIP
                     IKPLYTEADLEEIEHLGSFPGLEPYVRGPRATMYTNRPWTIRQYAGFSTAEESNAFYR
                     QNLAAGQKGLSVAFDLATHRGFDSDHPRVVGDVGMAGVAIDSVEDMKILFDGIPLDQM
                     SVSMTMNGAVLPVMAGYIVAAEEQGVAPKDLAGTIQNDILKEFMVRNTFIYPPEPSMR
                     IVADIIGYTAEHMPRFNSISISGYHMQEAGANAVQEVAFTLADGIEYVRAALATGQEI
                     DAFAPRLSFFFCIGMNFFMEIAKLRAARVLWHELMSQFKPQNPTSLMLRTHCQTSGVS
                     LQEHDPYNNVVRTTIEAMAAVLGGTQSLHTNAFDEAIALPTPFSARIARNTQIIIAEE
                     SQMTRTVDPLGGSYYVESLTNSIVEAAREIIDEVEAMGGMTKAVVAGMPKQRIEEASA
                     RRQARVERGEDVIVGVNKYKLEKDDSVFDVLAVDNSKVRDAQLKRLARIRASRDETAT
                     QAALDALTEAARTGEGNLLDLSVAATRLRATVGEVSTALEKVWNRHTPVARMTPGVYA
                     DAHQEDEQFVRVRDEVKVFTELEGRPPSILVVKMGQDGHDRGAKVISNAFGDLGFEVK
                     MGPLFQTPAEAASQATDEAVNVVGVSTLAGGHKTLVPEMIEALKAAGNEDVIVVVGGV
                     IPQKDYQFLYDCGVHAIYGPGTNIPEAASDVLQLIPGKNR"
     gene            complement(4062..4472)
                     /locus_tag="AC16UZ_16070"
     CDS             complement(4062..4472)
                     /locus_tag="AC16UZ_16070"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HIR6248255.1"
                     /translation="MITENADQDQFITDHRWGVLTTLRQDGQPVSSVVAYARDGDTLV
                     VSTPGTTFKARSAGRDGRATLCVITNQEPFNFVSVEGRVDVETDELVRNTRLVFENIE
                     GTGFVEPEDLEGWLAAEQRVILRIHPQRVFGVIR"
     gene            complement(4558..6060)
                     /locus_tag="AC16UZ_16075"
     CDS             complement(4558..6060)
                     /locus_tag="AC16UZ_16075"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR6248256.1"
                     /translation="MKQKWYLLALVSAALVCQIAQAGVDTVRVSEAFREASVAANSDR
                     ELAPDQPPDQRLAAGMQLTAHVARSHTRDFIRRPLTSVGNLLAATFTGATGIVKELAL
                     DFVLVPRLASKAARPLSSSRGMDIEEWENDLDRITGTERTRGQMKLLVDGEAFFDRLI
                     AEIAAARRSIEIRTYIFDNDDYAVAIADLLRRRSREVAVTVMADGLGTIGGTLATADS
                     QPADYVMPVSMGAYLKEGSGVRLQMLGNTWFMGDHTKTYIFDERVAFLGGMNIGREYR
                     YDWHDIMVELTGPVVGELSSEAAQAAREDRLGDLALLGSTKRTPRTDGAEGGQELRLL
                     RTRPHDAQIYRAQLEAIRRAEKHIFVQNAYLGDDQIIYELAKARRRGVDVRVILPARP
                     DSELVRRSNALAINTLRRFGVRVYLYPGMTHVKAAIYDGWACFGTANFDRLSLRINKE
                     VNIATSDPAFVGSLRAQVFDVDFARSREVVDDEPTGASDHLYERIVDILL"
BASE COUNT         1143 a         2127 c         1996 g         1134 t
ORIGIN      
        1 ggtgcggcca cccgcgccag acccacgggc acgatcgcca ggcagatgag ggcagcgcct
       61 gccatgaaga gctccaggct catgggtgag gctatgccga ggaaagcctg ccccacggac
      121 atccccagca ggcagatgac cgtgtagaga gccagtatcg ttccccgatt gttcgatggc
      181 gtcacgtcca ccagccagct ttcgatcacc atgtagagac cggacagcgc gaacccggta
      241 acgaaccgga agatgagcca ggcaggcaag gtcacgagca acccggcagc cagcagcgca
      301 acggtggcca aggcccccat caccatgaaa gtgcgaatgt ggccgaccct gccgaccacc
      361 ggtggaatca gaacgcagcc caccacgaaa cccagaaaat aggccgaccc gctggtaccg
      421 atggcggaag ccgaccaacc cagaccctct gcgtgcaccg gcaggagcgt aagctggaga
      481 ccgtggccga tcagcaggat ggcgacagcg gtcagcagag cgctcagacg ggcgatcgtt
      541 ccgcgcatgg ttctgcctac cgccctcgcc ggggctcgaa ttcccaggac atggtgtcca
      601 ggtaacggcg cagctgacca gcggtgcgaa agcgctccga gatgagcgaa cgcttgagac
      661 cgcgacagat cgccttgatc tcaggcgatt gccgggcgta gaaacgctgg ccgcccaggg
      721 catcgtaaaa gatccggatg gcatcgcaga cgtcgtcctt tacgctcaac gactttcgct
      781 gatcgaggtg gtagacgtcg atgagccgga catcgaaatc gagaccgacc cgacgcacga
      841 tgacattgcc cgcgtgcaga tcgccgtggt actcgcgacg ctcgtgaatc ggttcgagcg
      901 ccagcgccag ggcccgcagc aggtgcagcg cctcgaagcg gtgcattcgc ccacccggct
      961 gccgcgcgat gaactcgctg agcagatcgc cctccacgta ctccgagatg agcaccgtca
     1021 cggcctgccg cctgacggtg atcgtttcct gggccacgta ctggatcagc gccgggcagt
     1081 ccctgagctt gtgtagcttt tccgccgtgc ggcgcgccac cttattgccc cggtttcggt
     1141 gcggaaagaa cactttggcc gtccgctcga taccggtggc cgtctcgcgg atgagataga
     1201 cctcactttc ccagccggct ccaagctggc tgacgacctg atatcgggcc gtgatcttgc
     1261 ggcccggcgg aaatcgaagc tgtcgatgcg gacaagggtg gtcaccgctg catcaagcct
     1321 ctcgggcgct caggggctgg ctacgatccg ggcacgttca ggcagcaact tcttcgagct
     1381 cgatcagcgt gccgcagaaa tccttggggt gcagaaagac cacgggcagg ccatgagccc
     1441 ccgtcgacgg cttgccgtcc ccaagcaccg tggcgccctc cgcgacgagg tgcgcgatgg
     1501 cggcttcgat gtcctggacc tcgtagcaca ggtgatgcat cccgcccttc gggttccgct
     1561 cgaggtagtt cgccaccgga gatccgtcac ccagcggatg tagcagctcg atcttgctgt
     1621 tgggcagctc gacgaagacc gtcgtgacgc cgtgatccgg gagcggcacg ggatcggaca
     1681 ccgtggcgcc cagcgtgtcc ctgtagagtg ccgccgcgga ttcgaggtcc gggacgacga
     1741 tggcaacgtg gttcagctta ccgatcattt gctcttccgt tccgtgttca ggttgttcat
     1801 cggttcttgc ccggaatcag ctgcagcacg tcgctggccg cctccggtat gttggtgccc
     1861 ggcccgtaga tggcgtgcac gccgcagtcg tagagaaact gatagtcctt ctgcggaatc
     1921 accccgccca ccacgacgat cacgtcctcg ttacccgccg ctttgagcgc ttcgatcatc
     1981 tcgggcacca gcgtcttgtg tcctccagcc agggtcgaga cgcctacgac gttcaccgcc
     2041 tcgtcggtcg cctgagacgc ggcctccgcc ggggtctgga acagcggccc catcttcact
     2101 tcgaagccaa ggtcgccgaa agcattggag atgaccttgg ccccgcggtc gtggccgtcc
     2161 tggcccatct tgaccaccag gatgctcggc ggccgccctt cgagctcggt gaacaccttg
     2221 acctcgtcgc gaacccgcac gaactgctcg tcctcctgat gggcgtcggc atacaccccc
     2281 ggggtcattc gcgctacggg cgtatggcga ttccagacct tctccagcgc ggttgagacc
     2341 tcgccgacgg ttgcccgcag gcgcgtcgct gccacgctga gatccagcag gttgccctcg
     2401 cccgtcctgg cggcctcggt gagcgcgtcc agggcggcct gagtggcggt ctcgtcccgg
     2461 gaggcgcgaa tgcgcgccag ccgcttgagc tgcgcgtcgc gcaccttgct gttgtccacc
     2521 gcgagaacgt cgaagacgga gtcatccttt tcgagcttgt acttgttcac ccccacgatt
     2581 acgtcctcgc cccgctcgac ccgggcctgg cggcgcgccg aggcctcctc gatgcgctgc
     2641 ttgggcatgc cggccacgac cgccttggtc atgccgccca tggcctcgac ctcgtcgatg
     2701 atctcacggg ccgcctccac gatggagttg gtcaggcttt ccacgtaata gctgccgccc
     2761 agggggtcga ccgtgcgggt catctgggac tcttcggcga tgatgatctg ggtgttgcgg
     2821 gcgatgcgcg cggagaacgg cgtaggcagc gcgatggcct cgtcgaacgc gttggtatgc
     2881 aggctctggg tacccccgag cacggccgcc atggcctcga tcgtcgtcct gaccacgttg
     2941 ttgtagggat cgtgctcctg cagcgacacc cccgaggtct ggcagtgggt acgcagcatc
     3001 agcgaggtag ggttctgcgg cttgaactga ctcataagct cgtgccagag aacgcgcgcc
     3061 gcccgcagct tggcgatctc catgaagaag ttcatcccga tgcagaagaa gaacgacaga
     3121 cgcggcgcga acgcgtcgat ctcctgcccc gtcgccaggg cggcacggac gtactcgatc
     3181 ccgtcggcca gcgtgaaggc cacttcctga accgcgttgg cccccgcctc ctgcatgtga
     3241 tagccggaga tggagatgga attgaagcgc ggcatgtgct cggcggtgta gccgatgata
     3301 tccgccacga tccgcatgct cggctcgggc gggtagatga acgtgttgcg gaccatgaac
     3361 tccttgagga tgtcgttctg gatggtcccc gcgagatcct tgggcgccac cccctgctcc
     3421 tcagcggcga cgatgtagcc ggccatcacc ggcagaacgg cgccgttcat ggtcatggac
     3481 accgacatct gatccagcgg aattccgtcg aagagaatct tcatgtcctc cacggagtcg
     3541 atggcgaccc ccgccatgcc gacgtcgccc acgacgcgcg ggtggtccga gtcgaacccc
     3601 cggtgggtgg ccagatcgaa ggccaccgac aggcccttct gccccgcagc caggttctgg
     3661 cggtaaaagg cgttggattc ctccgccgtg gagaagcccg cgtactgacg gatggtccac
     3721 ggacgattgg tgtacatggt cgcgcgcggc ccgcgaacgt atggctcgag accggggaat
     3781 gagcccagat gctcgatttc ctcgagatcc gcttcggtgt acagcggctt gatcgggatg
     3841 ccctccgggc tcacccaggt gagctcgtcg ggcggtttgc cgcgcagttc cttcgcagcc
     3901 agctctttcc aggtcgcctc gtcctgtccc gcagggtcgc tcttgcgtgt cgggttctct
     3961 gccatggttc ctacctcgag cctcaggagt cgcgcccagc gcgcaaacgg ccggcgatta
     4021 tagcaacgtc cagccccgcg gggaccggcg gatgaccccc gtcagcgaat aaccccgaac
     4081 acgcgttggg ggtgtatccg cagaatcacc cgctgctcgg ccgcaagcca gccctccaga
     4141 tcttccggct cgacgaagcc ggtgccctcg atgttctcga acaccagccg cgtgttgcga
     4201 acaagctcgt cggtctccac gtcgacccgc ccctccaccg agacgaaatt gaacggctcc
     4261 tgattggtaa tgacgcagag cgtggcccgc ccgtcgcggc cggccgaacg cgccttgaac
     4321 gtcgtgcccg gcgtgctgac cacgagcgtg tcgccgtcgc gcgcgtaagc caccaccgag
     4381 ctgaccggct ggccatcctg gcgcagcgtc gtcagaaccc cccagcgatg atcggtgatg
     4441 aactgatcct gatcggcgtt ctcggtaatc aaggcgaccc tccacaagca ttcctgtcca
     4501 gaccatcgac gatagcccaa caccgttcag cgaccaaggg tcttgcgccc gcagcgtcta
     4561 caggaggatg tccacgatgc gctcgtagag atggtcggac gctccggtgg gctcgtcgtc
     4621 cacaacctcg cgcgaccggg cgaagtccac gtcgaacacc tgggcgcgaa gcgacccgac
     4681 gaacgcggga tcggacgttg cgatgttcac ctctttgttg atgcgcaggc tgagccggtc
     4741 gaagttggcg gttccaaagc acgcccagcc gtcatagatg gccgctttca cgtgggtcat
     4801 cccgggatag aggtagacgc gaacgccgaa gcgtcgcaat gtgttgatcg ccagggcatt
     4861 gctgcgccga accagctcgc tgtcgggccg cgccggcagg atcacccgca cgtcgacccc
     4921 gcgtcgccgc gctttcgcga gctcgtagat gatctggtca tctcccaggt aagcgttctg
     4981 cacgaaaatg tgcttttccg cccgccggat ggcctcgagc tgcgctcggt aaatctgcgc
     5041 atcatgaggg cgcgtgcgta gcagccgcag ctcctggccg ccctccgcac cgtccgtcct
     5101 gggggtcctt ttggtggagc cgagcagggc cagatcgcca agccggtctt cccgggccgc
     5161 ctgagcggcc tcgctggaca gctcgcccac cacaggcccg gtgagctcga ccatgatgtc
     5221 gtgccagtcg tagcggtact cccggccgat gttcatgccg ccaaggaacg ccacccgctc
     5281 atcgaagata taggtcttcg tgtgatcgcc catgaaccag gtgttgccaa gcatctgcag
     5341 acgcacgccg gaaccttcct tcaggtaggc acccatggac acgggcatga cgtaatcggc
     5401 cggttgcgag tcggccgttg ccaacgtgcc accgatggtg ccgaggccgt ccgccatcac
     5461 cgtcacggca acctcgcgcg atcgccgccg cagcagatcg gcgatggcga ccgcatagtc
     5521 gtcgttgtcg aagatgtagg tccggatctc gatggatcgg cgcgcggccg ctatctcggc
     5581 gatgagcctg tcgaagaacg cttcaccatc caccagaagt ttcatctgcc cccgcgtgcg
     5641 ttcggttccc gtaatccggt ccaggtcgtt ctcccactcc tcgatatcca tgcccctcga
     5701 cgaggaaagt ggacgagccg ccttgcttgc cagcctgggt accaggacga agtcgagagc
     5761 aagctcctta acgatccccg tggctccagt aaacgtggcc gcaagcagat tgcccaccga
     5821 ggtaagcggc cgccgaatga agtcacgagt gtggctgcgc gccacgtggg cggtgagctg
     5881 catgccggcg gcaagccgct gatcgggcgg ttgatccggc gcgagctccc gatccgagtt
     5941 ggcagccacc gatgcctccc ggaacgcctc agacacgcgc accgtatcga ccccggcctg
     6001 ggcgatttgg caaaccagag ctgcggacac cagtgccagc aaataccact tctgcttcac
     6061 ctcgaattct cctcaccgtt cagatttccg aggttcaacg gtagatgccg cgcggcagac
     6121 ccgcctgagg gggcgcgaaa agaatgattt gggtttctta gggttcttta ggccgagcgt
     6181 cgggggggcc gccataggag agctcaatca aaaaagcccg cacaaggcgg gccgttttga
     6241 ttggcgtccc ctaggggagt cggaacccgc cggccgcttc gcgaccttac ggcagtttcg
     6301 ctacgcaaaa ctgcggcgaa cccactccgc caaagaagag cgcaatcaaa aaagcccgca
     6361 caaggcgggc cgttttgatt ggcgtcccct aggggagtcg
//
LOCUS       DBNHOX010000740         3007 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_189703, whole genome shotgun sequence.
ACCESSION   DBNHOX010000740 DBNHOX010000000
VERSION     DBNHOX010000740.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_189703"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..354
                     /locus_tag="AC16UZ_16080"
     CDS             160..354
                     /locus_tag="AC16UZ_16080"
                     /inference="COORDINATES: protein motif:HMM:NF024851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR6248257.1"
                     /translation="MKVKIDYEKCYKSGECYYNHPELFRMGDEGYPVVLVDQISDDAT
                     RRHAEEAIEVCPAVAITLED"
     gene            complement(433..2778)
                     /locus_tag="AC16UZ_16085"
     CDS             complement(433..2778)
                     /locus_tag="AC16UZ_16085"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6248258.1"
                     /translation="MNIRSCGGTAAFVLAGLAAGLLESRPVSAAEEESALVHEEVIVT
                     ARRREESLQTVPVAVSVLTGEELVEQGGMKIDAIGKLVPNVHFEAAGGTSGVKSPIVF
                     IRGMGQNDFIPVEDPAVGIYLDGVYMGRNIGSVFDLVDIERVEVLRGPQGTLFGRNTI
                     GGAVNIISKKPGDEFGGSLKVAGGDEGYLEAQGSVNLPLGDRAAARLSGFVRERDGYV
                     KALQHDDLELGSDDIWGLRGSLRAELTDSFSLDIALDYSKAEETPGAISPIAGIGGFD
                     GDVITLGLPAQPFAFFWNAIWSGVAPPGCTTGTGQASNSACYGPVWNTDDPYKINSSY
                     VDNTGTQIDPEQSVEVWGGNVTATWQIGSVELKSITSYREFDIGFFNDLDFSPYILFA
                     NNHDEYTQEQWSQELQLSGTAMSDRMDYLLGFYYFDEEGHEAIFNQISFAPPLAAPPD
                     FFFQYLDRFIDNDSWAVFGQVNFDLTETLTLTVGGRYTESNKDFALITERRVGPISDQ
                     GGKLTTEEFTPLVSLAWQVQEDVMVYATWSEGYRDGSYAARFTGAVPDPLPNYDPEYV
                     NNYEIGVKTTLLDGRMRLNATAFLMDYEDMQINASSDEVTTSSTKANLGDATISGVEL
                     EISALVTENLTLGLNLGILDDDIDSLKGTLISNTVVIDETSDLPNTPNWTLSLMAKYE
                     IPLNNGSRLAFRADYVAKDDYYSRAENIPELLIDNYENLNVSGTFISPGGDWEVGVGV
                     RNATDEFYYQSATPFATFGLSFGQPVRPRTWYGSLTYNFGN"
BASE COUNT          519 a          930 c          963 g          595 t
ORIGIN      
        1 catggcgcgg caggaatcgg ccatcgcgtg caccaggctg atggaagcca tccgcaaccc
       61 gcggccgaag ttcgccgagc atccggggat cagctcgcct tctatcgatt ccggcgggtt
      121 ccggtcacct caggaactgt ggatggagtt cgaccgccca tgaaggtgaa gatcgactac
      181 gagaagtgct acaagtcggg cgagtgctac tacaaccatc ccgagctgtt tcgtatgggg
      241 gacgagggct acccggtggt gctggtggac cagatcagcg atgacgccac gcgcaggcac
      301 gccgaggaag caatcgaggt ctgcccggcg gtggccatca cgctggaaga ctgaacccca
      361 tcacctgtgt gacgaggcaa aacgcccgac gcggtcgctg atgagcgcgt cgggcgcggc
      421 tttctggatg cttcagttcc cgaagttgta ggtcagcgat ccgtaccagg ttcgcggcct
      481 caccggctgg ccgaagctca gaccaaacgt ggcgaacggc gtggccgact ggtagtagaa
      541 ctcgtctgtc gcgttgcgca ccccgacgcc cacttcccaa tcaccgccgg gcgagatgaa
      601 ggtcccgctg acgttcaggt tttcgtagtt gtcgatcagc agctcgggaa tgttttcagc
      661 gcggctgtag tagtcgtcct tggccacata gtcggcgcgg aaggcgaggc gtgatccgtt
      721 gttgagcgga atctcgtact ttgccatcag ggacagcgtc cagttcggcg tgttgggcag
      781 gtcgctggtc tcgtcgatga cgacggtgtt cgaaatcagc gttcccttca ggctgtcgat
      841 gtcgtcatcg agaatgccca gattcagccc cagggtgagg ttctccgtta ccagcgctga
      901 gatctcgagc tccacgccgg agatggtcgc gtctcccagg ttggccttgg tcgaggacgt
      961 cgtcacctcg tcgctggacg cgttgatctg catgtcctcg tagtccatca ggaacgcggt
     1021 ggcgttcagc cgcatgcggc cgtccagaag cgtggtcttg accccgatct cgtaattgtt
     1081 gacgtactcg ggatcgtagt tgggcagcgg atccggaacc gcgccggtga atcgggccgc
     1141 gtagctgccg tcccgatagc cttccgacca ggtcgcgtag accatgacgt cttcctggac
     1201 ctgccaggcg agcgacacca ggggggtgaa ctcttcggtg gtcagcttgc cgccctggtc
     1261 ggagatgggc ccgacacgcc gctcggtgat cagggcgaag tctttgttgc tctccgtgta
     1321 gcgaccgcct accgtcaacg tcagggtctc ggtgaggtcg aagttcacct gcccgaacac
     1381 ggcccagctg tcgttgtcga tgaagcggtc caggtactgg aaaaagaagt caggcggcgc
     1441 agcaagcggc ggtgcgaagg agatctggtt gaagatggcc tcgtggcctt cctcgtcgaa
     1501 gtagtagaag ccgaggaggt agtccatgcg gtcgctcatg gcagttccgc tcagctgcag
     1561 ctcctgggac cattgctcct gggtgtactc gtcgtgattg ttcgcgaaca ggatgtaggg
     1621 cgagaaatcc agatcgttga agaagccgat gtcgaactcc cgataggagg tgatggactt
     1681 cagctccacg gagccgatct gccaggtggc cgtgacgttg cccccccata cctcgacgct
     1741 ttgctcgggg tctatctggg tcccggtatt gtccacatag ctcgagttga tcttgtaggg
     1801 gtcgtctgtg ttccagaccg ggccgtagca cgcgctgttg ctggcctggc cggtgccggt
     1861 cgtacaccca ggcggtgcaa cgccgctcca gatcgcgttc cagaagaagg cgaacggctg
     1921 ggcaggaagc ccgagggtaa tgacgtcgcc gtcgaacccg ccgatgccgg cgatgggcga
     1981 gatcgcgccc ggcgtctcct cggccttcga gtagtccaga gcgatgtcga ggctgaacga
     2041 atccgtcagc tccgcccgca ggctgccccg cagcccccag atgtcgtcgc tgccgagctc
     2101 cagatcgtcg tgctgaaggg ccttcacgta gccgtcgcgc tcgcgcacga agccgctcag
     2161 ccgggctgcg gcccgatcgc ccaggggcag gttcacgctg ccctgggctt cgaggtaacc
     2221 ctcgtctccg ccggccacct tcaacgagcc gccgaactcg tcgcccggct tcttggagat
     2281 gatgttgacc gcgccgccga tggtgttgcg gccgaacagg gtgccctggg ggcccctgag
     2341 cacttctacc cgctcgatgt ctacgaggtc gaatacgctg ccgatgttgc ggcccatgta
     2401 cacgccgtcg agatagatgc ctacggcagg atcttcgacc gggatgaagt cattctggcc
     2461 catgccgcgg atgaagacga tgggcgattt gacgccgctg gtcccgccgg ctgcctcgaa
     2521 atgcacgttg ggcacgagct tgccgatggc gtcgatcttc atgccgccct gctcgacgag
     2581 ttcctctccc gtcagcaccg aaacggccac aggcaccgtc tgcaggctct cttcgcgacg
     2641 ccgggcggtg acgatgactt cctcatgcac cagcgcggac tcttcctcgg cggctgagac
     2701 cggtcgactc tcgagcagtc cggctgccag gcccgccaat acgaatgcag cggttccgcc
     2761 gcaagatctg atattcataa ccccctcccc aggaagcttt gttgtctatc gcctcagcac
     2821 cccggtgttc gttcgcgagg gtgctcaccg tcttccatgc caagaccggg acgcgcaaat
     2881 tccttccctt ggccggatcg agggtcaatg atgttgacta ttgagcctgc cgtcaaccga
     2941 tggtccgatc cgggaagtgc cagcccatcg acttcggtgc ccgggccggg tcagtcggct
     3001 gccggcg
//
LOCUS       DBNHOX010000741         2586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_317751, whole genome shotgun sequence.
ACCESSION   DBNHOX010000741 DBNHOX010000000
VERSION     DBNHOX010000741.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_317751"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1704
                     /locus_tag="AC16UZ_16090"
     CDS             85..1704
                     /locus_tag="AC16UZ_16090"
                     /inference="COORDINATES: protein motif:HMM:NF013355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HIR6248259.1"
                     /translation="MTIRFLGKNVSGPFTIPSGIVATAVPIIEYLFRHVPQIGVMTTK
                     SIGLAPRTGNREPIYSQYAPGCFVNAVGLTNPGAERSAELFAALDVPEDRFLLTSIFG
                     GSVEEFVAVAKILAPVSDGLELNLSCPHAKGYGMEMGRDPELVREITAAVKAAVDVPV
                     VPKLTPNADNIGEIAQAAVAGGADALCAINTVGPGYYSAAGEPVLSNELGGMSGKGIL
                     PIGLKCVREIAGRVNVPVIGCGGISSAADVNAYRAAGAHIFGVGSALTGMTSPEITQY
                     FQTLAADLADDGNDAEARIQYRLDMGFRPYTLVTNTRVTDDICILTFDRPLDIKAGEF
                     VFLWIPGLGEKPFSALTDDPFSLVVIDLGQFTHALMDLAPGTRTYVRGPHGLPAEPPP
                     GARIVAVAGGTGLAAVYQLARDHGNADIFMGARTAERLYFIDECEAAATLHVATDDGS
                     CGHHGLVTELLAKHLETLSENELANTVFYNCGPEPMVHAAVEVQRRFCPDEQIFSAID
                     YLTKCGVGICGACTAPDGRRLCVDGPFLSAS"
     gene            complement(1808..>2586)
                     /locus_tag="AC16UZ_16095"
     CDS             complement(1808..>2586)
                     /locus_tag="AC16UZ_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6248260.1"
                     /translation="ASNDLMFYGLYSKGFRAGGFNGRPGGGQYLAAATPYDPETVDNF
                     EVGMKSEWLDGRMRLNVSAFMMDYQDKQEEQSVPVAGGTGQQTLVVNASEASINGVEV
                     DFSWLVSANFTIEGNLGLLDAEYDELEDPAFDPPLDLSDKKLRRAPDMTATISPVVTV
                     PIGPGQLIARAALRYVDDMELTFLNSPQSSVGSHTVLDASLTYTVNQLSISVWGLNLT
                     EDDSWTQAYDVGTSPAFAGLWTYSAIRPPRTYGAVVSWDF"
BASE COUNT          451 a          875 c          840 g          420 t
ORIGIN      
        1 tacgggtaga cgcgtggttc gattgggcgc gccgaacctg tagaatcccg ctccgaatcc
       61 cgacaccgct gagacccgcc cgccttgacg atccgattcc tgggcaagaa cgtatccggc
      121 ccgttcacca tcccatcggg gatcgtcgcg accgccgtgc cgatcatcga gtacctgttc
      181 aggcacgttc cgcagatcgg tgtgatgacc accaagagca tcgggcttgc accccgcacc
      241 ggcaatcgcg agcccatcta cagccagtac gcgcccggct gcttcgtcaa cgccgtgggc
      301 ctgaccaacc cgggcgccga acgttcggcc gagctcttcg ccgcgctcga cgtcccggaa
      361 gatcgctttc tgctcacctc catcttcggc ggcagcgtcg aggagttcgt ggcggtggcg
      421 aagatcctgg cgccggtctc cgacggcctg gagctgaacc tgtcctgccc ccacgccaag
      481 gggtacggca tggagatggg ccgggatccg gagctggtgc gcgagatcac cgcggcggtg
      541 aaagcagccg tggacgtgcc cgtggtgccg aagctcacgc cgaacgcgga caacatcggc
      601 gagattgcgc aagccgccgt ggccggcggg gcggacgccc tgtgcgccat caacaccgtg
      661 gggcccggct actacagcgc cgcaggcgag cctgtgctga gcaacgagct cggcggcatg
      721 tcgggcaaag gcatcctgcc catcggcctg aagtgcgttc gggagatcgc cggaagagtc
      781 aacgtccccg tgatcggctg cggtgggatc agcagcgcgg ccgacgttaa cgcctaccgc
      841 gcggcagggg cccacatatt cggggtgggc tcggcgctga ccggcatgac gtcgccagag
      901 atcacccaat acttccagac gctggcggca gacctggcag acgacgggaa cgacgcggaa
      961 gccaggatcc agtaccggct cgacatgggc ttccgcccgt acacgctcgt gacgaacacc
     1021 cgcgtcaccg acgacatctg cattctcacc ttcgaccggc cgctggacat caaagcgggt
     1081 gagttcgtct tcctgtggat acccggtctg ggcgaaaagc ctttctcggc gctgaccgac
     1141 gatccgttct cgctggtggt catcgacctg gggcagttca cccacgcgct catggatctc
     1201 gctccgggta ccaggaccta cgtgcgcggt ccccacggcc tgccggcgga accgccccca
     1261 ggcgcgcgca tcgtcgcggt cgcggggggc accggcctcg ccgccgtcta ccagctggca
     1321 agagaccacg gcaacgccga tatcttcatg ggtgcgcgaa cggccgaacg gctctacttc
     1381 atcgacgagt gcgaagcggc ggccaccctg cacgtggcca ccgacgacgg cagctgcggg
     1441 caccatggcc tcgtgaccga gctgctggcc aaacacctgg aaacgctgtc ggaaaacgag
     1501 ctcgccaaca ccgtcttcta caactgcggc ccggagccca tggttcacgc ggcggtcgag
     1561 gtccagcgcc ggttctgccc ggacgagcag atcttcagcg ccatcgacta cctcaccaaa
     1621 tgcggggtcg gcatctgcgg cgcctgcacc gcacccgacg gccggcgcct gtgcgtggac
     1681 ggcccgttcc tgagcgcgag ctgacgcaaa gcccactgcg cgttcctccc cagaggccga
     1741 tcagcgccta cccttgggca gaaaaaagcc ctccgaagag ggcttttcgt gccggcactg
     1801 ccgaaagcta gaagtcccag ctgaccaccg cgccgtaggt gcgcggcggg cggatggccg
     1861 agtaagtcca cagaccggca aaggcggggc tggtgcccac gtcgtaggcc tgggtccagg
     1921 agtcgtcctc ggtcaggttc agcccccaga cgctgatgct gagctggttc accgtgtagg
     1981 tcagcgacgc atccagcacc gtgtgggagc ccaccgagct ctggggcgag ttcaggaacg
     2041 tcagctccat gtcgtccacg tagcgcaggg cggcccgggc aatgagctgg ccgggcccga
     2101 tgggaacggt caccaccggc gagatggtgg ccgtcatgtc cggcgctcgg cgcagcttct
     2161 tgtcggaaag gtcgagggga gggtcgaacg ccggatcctc gagctcgtcg tactccgcgt
     2221 cgagcagccc caggtttcct tcgatagtga agttcgcact gacgagccag ctgaaatcga
     2281 cttccacccc gttgatggat gcttccgagg cgttgacgac cagggtctgc tggccggtgc
     2341 ccccggcgac aggcacgctt tgctcttcct gcttgtcctg gtagtccatc atgaaggcgg
     2401 agacgttcag acgcatgcgg ccgtcgagcc actcgctctt catgcccacc tcgaagttgt
     2461 ccacggtttc tggatcgtaa ggggtagccg cggccagata ctgaccgcca ccgggacggc
     2521 cgttgaagcc ccctgcccgg aagcccttgg agtagaggcc gtagaacatg aggtcgttgc
     2581 tggcct
//
LOCUS       DBNHOX010000742         2324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1216950, whole genome shotgun sequence.
ACCESSION   DBNHOX010000742 DBNHOX010000000
VERSION     DBNHOX010000742.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1216950"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="AC16UZ_16100"
     CDS             complement(<1..459)
                     /locus_tag="AC16UZ_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248261.1"
                     /translation="MSTKDSADSEDELLGDLESLRSILDEEDELMPQDDEAVPLLDDV
                     VADAADAEEPARASPGGLDDDLFQALLSDDWRESAAAIIEQAPAALGQAVAWKPREIA
                     ALRQALRLKQDETLNQWISDALERHAEALQAELLEAFSGELKPAVEGILRR"
     gene            complement(459..896)
                     /locus_tag="AC16UZ_16105"
     CDS             complement(459..896)
                     /locus_tag="AC16UZ_16105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HIR6248262.1"
                     /translation="MTRIDFYILQDVDLDAMRRFACRLAGKAITGGHEVHIHTQSAED
                     ASIVDELLWSYPDDRFIPHETLDDSGRAAVAAPVTLGWTEPDGLDGVLINLSDEIPRF
                     FGRFDRVAEVIPRSRRDAGRDRYKFYRDRGYPLFHHELDDWEA"
     gene            complement(901..>2324)
                     /locus_tag="AC16UZ_16110"
     CDS             complement(901..>2324)
                     /locus_tag="AC16UZ_16110"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR6248263.1"
                     /translation="STPCLITGLKTARRVARELGHEVVFTAATSDFKDSAGQVQVVGL
                     PSSGPVRRILVTGGADDDLSAESYRKVIGAAATALKSVPARSALWALNHARVDGQDAY
                     WKASAGIFALSTALYAFDEHKSTRNDDAGITIRTVQVHADARTRSNVQRAVRHGNALK
                     SGLDLARNLGNQPPNVCNPPYLVREARKLARRDKVTVTSLDEKRMRELNMGAFLAVSA
                     GSETPGRMIIVRYNGGRRQDAPVVLVGKGITFDTGGISLKPPAAMDEMKYDMCGAATV
                     LGATLAAVEAALPINLVTIVAAAENMPSGRATRPGDIVTTHSGKTVEILNTDAEGRLV
                     LCDALSYARRYEPKAIVDVATLTGACIVALGSHASAVYSKDDDLAEELVNAGEWTGDR
                     AWRMPLWDEYQAQLKSRFADVANIGGPQAGSVTAACFLSRFTDSMRWAHMDVAGSAFQ
                     GGANKGATGRPVGLLFRFLIGQS"
BASE COUNT          368 a          746 c          797 g          413 t
ORIGIN      
        1 gcgacgcaag atgccttcta ccgcgggctt cagctcaccg gagaaggctt cgagcagctc
       61 ggcttgcagc gcttcggcgt ggcgctcgag tgcatcactt atccactgat tcagggtttc
      121 gtcctgcttc agacgcaggg cttgccgaag ggccgcgatc tcccggggct tccaggcaac
      181 ggcctggccg agggcagcgg gcgcctgctc gatgatggcg gcggccgatt cgcgccagtc
      241 atcgctcagc agcgcctgga acaggtcgtc gtccaatcct cccggactgg cccgggcggg
      301 ctcctccgcg tcggccgcat ctgcgaccac gtcgtccaga agcggaacgg cctcgtcgtc
      361 ctggggcatc agctcgtcct cctcgtccag aatcgaacgc agggattcga ggtcgcccag
      421 cagctcgtcc tcggagtctg cagagtcttt ggtgctcatc acgcttccca gtcgtcgagc
      481 tcgtggtgga acagcggata tccgcggtcc cgatagaact tgtatctatc ccgtcctgcg
      541 tcccggcgtg accgcgggat cacctcggcg acccggtcga agcggccgaa gaagcgcggg
      601 atctcgtcgc tcaggttgat gagcacgccg tccagaccgt cgggctcggt ccaccccaga
      661 gtcaccggcg cggcgacggc cgcgcggccg gagtcgtcga gggtctcgtg ggggatgaag
      721 cggtcgtcgg ggtacgacca gagcagctcg tccacgatgc tggcatcctc ggcgctctgg
      781 gtatgtatgt ggacctcgtg gccgcccgtg atggccttgc cggcaagacg gcaggcgaat
      841 ctgcgcatgg cgtccagatc gacgtcctgg aggatgtaga agtcgatgcg ggtcatgggg
      901 tcaagactgc ccgatgagga agcggaacag aagacccaca ggccggccgg tggctccctt
      961 gttcgctccg ccctggaatg cgctgccggc cacgtccatg tgtgcccagc gcatggagtc
     1021 cgtgaaccgg gataggaaac aggccgcggt gacggagcct gcctgggggc ctccgatgtt
     1081 ggcgacgtcg gcgaagcggc ttttcagctg ggcctggtac tcgtcccaca ggggcatgcg
     1141 ccaggcccgg tcgcccgtcc actcgccggc gttcaccaat tcttcggcga gatcgtcgtc
     1201 tttcgagtac acggcggagg cgtgggaacc cagagcgacg atgcaggccc cggtgagggt
     1261 tgccacgtcc acgatggctt tcggctcgta gcggcgcgcg taggaaagcg cgtcgcaaag
     1321 caccaggcgc ccctcggcgt cggtgttgag aatttccacc gtcttgcctg aatgcgtggt
     1381 gactatgtcg cctggccgcg tggcccgccc gctgggcatg ttctccgcgg ccgcgacgat
     1441 ggtgaccaga ttgatgggca gggccgcctc cacggcggcc agggtggcgc caagtaccgt
     1501 ggcagcgccg cacatgtcgt acttcatctc gtccatggcc gcaggcggct tcaggctgat
     1561 accgccggtg tcgaaggtaa tgcctttgcc taccagcacc acgggcgcgt cctgccgccg
     1621 gcccccgtta tagcgcacga tgatcatgcg tccgggtgtt tcgcttcctg cagagacggc
     1681 gaggaaggca cccatgttca gctcgcgcat gcgcttctcg tccagggagg tgacggtcac
     1741 cttgtcacgc ctggccagct tgcgggcctc gcgcaccaga tagggaggat tgcagacgtt
     1801 cggcggctgg ttgcccagat tgcgggcaag gtcgagcccg gacttcagag cgttgccgtg
     1861 ccggacggcg cgctggacgt tgctgcgggt gcgggcatcg gcgtgcacct gcactgtccg
     1921 aatcgtgatg ccggcatcgt cgttgcgcgt ggacttgtgc tcatcgaatg cgtacagggc
     1981 ggtggaaagg gcgaagatcc cggcgctggc tttccagtag gcatcctgcc cgtcgacccg
     2041 ggcatgattg agcgcccaca aagccgatcg cgccgggaca gacttcagcg cggtggcagc
     2101 cgccccgatg actttacggt acgactccgc cgagaggtcg tcgtcggcgc cgccggtgac
     2161 cagaatacgc cgcacaggtc cggatgaagg aagcccgacc acctggacct ggccggcgct
     2221 gtccttgaag tccgaggtcg cagcggtgaa cacaacctcg tgaccgagtt cccgggctac
     2281 ccggcgggcg gtcttcaagc ccgtgatcag gcagggagtg gaca
//
LOCUS       DBNHOX010000743         3694 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1153842, whole genome shotgun sequence.
ACCESSION   DBNHOX010000743 DBNHOX010000000
VERSION     DBNHOX010000743.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1153842"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1318)
                     /locus_tag="AC16UZ_16115"
     CDS             complement(83..1318)
                     /locus_tag="AC16UZ_16115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6248264.1"
                     /translation="MKTKIFDRFLTNGTLAIVDADGSSRQFGCGAPFATMRLNRRGTL
                     TRILRNPELNLGETYLAGEWDVIEGSLHDLLTILRTNLERHAGRSRLARALALMLVPW
                     NHVAASHRNVRHHYDLDETLFRACLDTNMHYSCAYYREANMSLEEAQQAKCDHIAAKL
                     CLRPGMRVLDVGCGWGSLAMELAERHDVTVMGVTLSPEQLRVARSTARLRGLDNHVRF
                     ELQDYRQVEESFDRIVSVGMFEHVGRRHYDEFFGKVEALLADDGVALLHTIGASSPPS
                     VVNPWIERHIFPGGYIPSLSDVAPAVERSGLITADVEVLRHHYALTLQEWNRRFQQRR
                     AEFAQSKGEAFCRMWEFYLLACQTAFEVSDLVVHHWQLAKRNLSVPTTRNYLYETQPY
                     VKIPGETAAEEITLRQHQG"
     gene            1475..2209
                     /locus_tag="AC16UZ_16120"
     CDS             1475..2209
                     /locus_tag="AC16UZ_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HIR6248265.1"
                     /translation="MAHEIDYRICGESMQYLEVELDPGESVVAEAGTMMYLSEGIAYE
                     ARLGDGSASGVVGKLFGAAKRLLGSESLFMTHFTNEADGPRQMGFAAPYPGQVVAVDL
                     AQAGGEIVCQKDAFLCAAMGTSVSVAFSKRLGAGFFGGEGFILQRLAGDGTAFIHAGG
                     TVLEKRLEGEKLRVDTGCIVGFEPGIDYDIQLAGGLKSMMFGGEGLFLATLEGHGRVW
                     LQSLPFARLADRVLENAVHLERKGES"
     gene            complement(2238..3239)
                     /locus_tag="AC16UZ_16125"
     CDS             complement(2238..3239)
                     /locus_tag="AC16UZ_16125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6248266.1"
                     /translation="MKAITSITGIDLNEVGPLTRDLEARGFSGVSTQENRFNPFLPLA
                     VAATQSSTLELRTSVAIAFARSPMVSANLGWDLQAASRGRFTLGLGSQVKGHNVRRFS
                     VPWSPPAPRLREYVRALRAIWDCWQNGTPLDFAGEHYQFNLMTPNFTPPPLATAPPRV
                     QLAGVGPATMKVAAEECDGVMLHGFCTRRYVEEAVIPRLETGLAAAGRAREDFEISGG
                     GFVATGKDDAAVAEMFEWVRTRVGFYGSTPSYWPVFEVHGLEDLGHKLNDMSKLGQWD
                     AMAGEISDDVVRLFAAVGRFDEIAGAIEERFGGAVDVVALPASAPPDLLEDIARIAC"
     gene            complement(3244..>3694)
                     /locus_tag="AC16UZ_16130"
     CDS             complement(3244..>3694)
                     /locus_tag="AC16UZ_16130"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6248267.1"
                     /translation="IERAALLLVCAEIAGGLCGVLHTIVGYLTTRRQFDRLIGSYQAL
                     KHPTAEILLAAERSKSHLYHAATTIGRGGREAEVALRMAKAQGSEAFAWAGDRAVQFH
                     GGFGFTYECDAQLFLRRALWCQYQLGDERYHRRKLAPLLLAATDTEH"
BASE COUNT          621 a         1157 c         1268 g          648 t
ORIGIN      
        1 ccgactacgc agcgcggctg ccgctctacg tcatcgagga tgagtttccg ggtctgcttg
       61 gcgccgttgc ctgcctggcc gatcagccct gatgctgcct gagggtgatc tcttcggccg
      121 cggtttcccc ggggatcttc acgtacggct gcgtctcgta cagatagttg cgcgtggtgg
      181 gcacgctcaa gttgcgcttg gccagctgcc agtggtgaac caccagatcc gatacctcga
      241 aggcggtctg gcacgcgaga aggtaaaact cccacatgcg gcagaacgcc tcgcctttgc
      301 tctgagcgaa ctcggcgcgc ctttgctgga accggcggtt ccactcctgg agcgtcagcg
      361 cgtagtgatg acgaaggacc tccacgtcgg cggtgatcag cccgctgcgc tccacggctg
      421 gcgccacgtc ggagagtgaa gggatgtagc cgccgggaaa aatgtggcgc tcgatccacg
      481 ggttcaccac gctgggcggg ctggacgccc cgatggtgtg cagcagcgca acgccgtcgt
      541 ctgcgagcag cgcttcgacc ttgccgaaga actcgtcgta gtggcgacgg ccgacgtgct
      601 cgaacatgcc gacggagacg atcctgtcaa agctctcctc tacctgccgg taatcctgaa
      661 gctcgaagcg cacgtgattg tcgaggcccc gcaggcgggc cgtgctccgg gccacacgca
      721 gctgctccgg cgagagggta acccccatca ccgttacgtc gtgccgctcg gcgagttcca
      781 tcgccaggct gccccagccg cagccaacgt cgagaacgcg catgccgggg cgcaggcaga
      841 gcttggccgc gatgtggtcg cacttggcct gctgtgcctc ctccaggctc atgttcgctt
      901 cgcggtagta cgcgcacgag tagtgcatgt tggtgtccag gcacgcgcga aagagcgtct
      961 cgtccagatc gtagtgatgg cggacgttgc ggtggctggc ggccacgtgg ttccagggga
     1021 cgagcatgag cgcgagcgcc cgggcaagcc gcgatcgacc ggcgtgacgc tcgagattcg
     1081 tgcgcaaaat ggtgagcaga tcgtgcagag agccctcgat gacgtcccac tcgcctgcca
     1141 ggtaggtctc gcccaggttg agctcgggat tgcgcagaat ccgggtcaac gtcccgcgcc
     1201 ggttcaagcg catcgtggcg aacggagcgc cgcagccgaa ctgacggctt gaaccatcgg
     1261 cgtcgacgat ggccagcgtc ccgttggtga gaaaccgatc gaagatcttt gttttcacga
     1321 cgtcatgccc cctgctcggc ggccggttct ttgccagcca tcccctcaca tggcaataat
     1381 tatcgcgctg cgggtgttat ttcaactaga ccttttggcg tcgccggggg ccagaaggtg
     1441 caggtcgggg cttgagaacg aggaggaccg cgcgatggcg cacgagatcg actaccggat
     1501 ttgcggtgaa tccatgcagt acctcgaagt agaactcgat cccggggagt ctgtggtcgc
     1561 cgaagcgggc accatgatgt atctgagcga gggcattgcc tacgaggcac ggctcgggga
     1621 cgggtcggcc agcggggtgg tgggtaaact cttcggtgcg gccaaacggc tgctcggcag
     1681 cgagtcgctg ttcatgacgc acttcaccaa cgaagccgac ggcccgcggc agatgggctt
     1741 tgcggctccg tatcccgggc aggtggtcgc ggtggatctg gcacaggcag gtggcgagat
     1801 cgtctgtcag aaggacgctt ttctctgcgc ggcgatgggc acctccgtgt ccgtggcgtt
     1861 cagcaagcgt cttggtgcgg gcttcttcgg tggcgagggc tttatccttc agcgtctggc
     1921 cggtgacggc acggcgttca ttcacgccgg gggaaccgtg ctagaaaaac gtctcgaggg
     1981 cgagaagctt cgcgtggaca ccggctgcat cgtcggcttc gagccgggca tcgactacga
     2041 catccagctt gccggcggcc tgaaatccat gatgttcggt ggcgagggcc tgtttcttgc
     2101 cactctggag gggcatggac gggtatggct gcaatccctg ccgttcgcgc ggctggccga
     2161 cagggtcctg gagaacgccg tgcacctgga acgcaaaggc gagtcctgaa ccggatccgg
     2221 cggctcctga gggggactca gcaagcgatc cgggcgatgt cctcgagcag gtcgggcggg
     2281 gctgaggcgg gcagggcaac cacgtccacg gcgccgccaa agcgctcctc gatggcacca
     2341 gcgatctcgt cgaaacggcc caccgcggcg aagagccgga ccacgtcgtc agatatttct
     2401 ccggccatgg catcccactg gccgagcttg gacatgtcgt tcagcttgtg gccgagatcc
     2461 tcgagcccgt gcacctcgaa tacgggccag taggaggggg tggaaccgta aaagcccacg
     2521 cgggttcgga cccactcgaa catctccgca acggcagcat cgtctttgcc cgtggcgacg
     2581 aacccaccgc cggagatctc gaagtcctcg cgcgcgcgcc cggcggcggc caggcccgtc
     2641 tcgaggcgcg gaatcaccgc ttcctccacg tatcggcgcg tgcagaaccc atgcagcatg
     2701 accccgtcac actcctcggc tgcaaccttc atcgtggcgg gcccgacgcc ggccagctgc
     2761 acccggggcg gggcggtggc cagcggcggt ggggtgaagt tcggcgtcat caggttgaac
     2821 tggtagtgct cgccggcgaa atcgagcggg gtcccgttct gccagcagtc ccagatggcc
     2881 cgaagagcgc ggacgtactc gcgcagcctt ggcgccggcg ggctccaggg aacgctgaag
     2941 cggcgaacgt tgtgcccttt gacctgactg ccgaggccga gcgtgaatcg gccgcgggaa
     3001 gccgcctgca ggtcccagcc gagattggcg gaaaccatgg ggctgcgcgc gaaggcaatc
     3061 gccacgctgg ttctcagctc cagggtcgac gactgggtgg ccgccacggc cagcgggaga
     3121 aaggggttga aacggttttc ctgggttgac acgccgctga acccgcgcgc ttcgaggtcc
     3181 cgggttaggg gcccgacctc gttgaggtcg atgccggtga tggaggtaat ggccttcatg
     3241 agctcaatgc tccgtgtctg tcgctgctag gagtagcggc gcgagcttgc gccgatgata
     3301 gcgttcgtca ccaagctgat actggcacca tagcgcccgg cgcagaaaca gctgggcgtc
     3361 gcattcgtag gtgaagccga agcccccgtg aaactgcacc gcccggtcac cggcccacgc
     3421 gaacgcctcg cttccctgtg ctttcgccat ccgcagggcg acctcggctt cgcgcccgcc
     3481 ccggccgatc gtggtggctg cgtggtagag gtgtgacttg ctgcgctcgg ccgcgagcag
     3541 gatctcggcc gtggggtgct tgagcgcctg gtagctgcca atgaggcggt cgaactggcg
     3601 ccgcgtggtc aggtagccga cgatggtgtg cagcacgccg caaagcccgc cggcgatctc
     3661 cgcgcagacg aggagcaggg ccgcccgctc gatc
//
LOCUS       DBNHOX010000744         3955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_124512, whole genome shotgun sequence.
ACCESSION   DBNHOX010000744 DBNHOX010000000
VERSION     DBNHOX010000744.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_124512"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="AC16UZ_16135"
     CDS             complement(<1..201)
                     /locus_tag="AC16UZ_16135"
                     /inference="COORDINATES: protein motif:HMM:NF021364.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HvfC/BufC family peptide modification chaperone"
                     /protein_id="HIR6248268.1"
                     /translation="MRSLPEIQAGFAAALRGDTAPGAADVAGDGLTATGRLRIYQHNS
                     RAMFEGALERSYPVLRRRVGEAY"
     gene            complement(185..1054)
                     /gene="bufB"
                     /locus_tag="AC16UZ_16140"
     CDS             complement(185..1054)
                     /gene="bufB"
                     /locus_tag="AC16UZ_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MNIO family bufferin maturase"
                     /protein_id="HIR6248269.1"
                     /translation="MSSDPETNSSGPIPAAAGIGLRSAHHEALLRERPRVGWIEVHTE
                     NYFHDGGPHVRDLERARELYPLSLHGVGMGLGSADPLDRSHIAKLRRAIERFAPSLVS
                     EHACWASHDGEHFNDLLPLPYTEESAAHLADRVAQAQDLLGRQILIENLSCYLSYKCS
                     RLTEWEFLAAVVERSGCGLLLDVNNAYVNSVNVGLDATALINGLPAGAVQEIHLAGHA
                     RRIVDGREILLDDHGSQVPEPVWTLSELALRRHGPVPTLIEWDTNLPELSVLVGEADR
                     AQGRLEAAHALAA"
     gene            complement(1062..1319)
                     /locus_tag="AC16UZ_16145"
     CDS             complement(1062..1319)
                     /locus_tag="AC16UZ_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BufA1 family periplasmic bufferin-type
                     metallophore"
                     /protein_id="HIR6248270.1"
                     /translation="MRNLALAAAVGSLISMGVTATAVAGDKAGKEKCYGIAKAGQNDC
                     AANGHSCAGQSKVDNSPTEWKFVPAGQCETMGGKTEPAKKM"
     gene            complement(1532..2878)
                     /locus_tag="AC16UZ_16150"
     CDS             complement(1532..2878)
                     /locus_tag="AC16UZ_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6248271.1"
                     /translation="MNYELIALLMFAMMMLMLVTGQRVFGAIGFVATVFALALWGKGG
                     AEMPFASTITLLNWYPMLTLPMFIYMGYMLSESGIASDLYKMFHVWAGPLPGGLAVGT
                     VLLMVAISAMNGLSVAGMAIGASVALPELLKRNYDKVMVTGVIQAGSSLGIMIPPSVV
                     LVLYGMIARQPVGQLWLAGVFPGLLLAALFVAYILIRCKFQPHLGPPLAKEERDQYSW
                     VDKMRLLRAGILPLVIIFSMTGLFLMGVTSLVESSAAGAAAATLAAWFKGRITREVMT
                     ATLKKTLAVSCMFMWVILAALAFGAVFDGLGAVRAIEALFLEKWGLEPWQVLVLMQLT
                     YILMGMFLDDTAMLIIVAPLYVPLIIALGFNPIWYGVLYTITCQIAYMTPPFGYNLFL
                     MRAMAPPEISLMDIYRSIIPFVLIMTLGLTLVILFPQIALWLPDQFYGHIADPALN"
     gene            complement(2875..3408)
                     /locus_tag="AC16UZ_16155"
     CDS             complement(2875..3408)
                     /locus_tag="AC16UZ_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR6248272.1"
                     /translation="MNSTVSKMPWPIRAYVRYVDALNKGVGTVMLFMIFVMMGVLLFG
                     SISRYVFNASFLWIIEMAQFLMAAYYLLGGGYSMQLEAHVRMDVFYDRWQPRTRAFWD
                     SLTALCIVFYLIYMIYGGWSSTAYALKYGQTNYSAWAPLMWPIKMVMTVGIALMLLQA
                     FSFFFKDLARFMGREIA"
     gene            complement(3581..>3955)
                     /locus_tag="AC16UZ_16160"
     CDS             complement(3581..>3955)
                     /locus_tag="AC16UZ_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 periplasmic-binding domain-containing
                     protein"
                     /protein_id="HIR6248273.1"
                     /translation="GWADVTNYFLTNNISGAWIGSYFANTEAWNKVPEHLQTLFKLCM
                     DSSHYYRQYWYWGGEAHLRVKGEKMKLTTIPAEEWATVEAEALKFWDEIATTSPRCAR
                     VIQIFKDYNDLMKKAGPPYRYS"
BASE COUNT          718 a         1282 c         1305 g          650 t
ORIGIN      
        1 atatgcctcg ccgacgcgtc ggcgcagcac ggggtagctg cgctcgagtg cgccttcgaa
       61 catggcgcgt gagttgtgct gatagatgcg cagccgtcct gtcgctgtca agccgtcgcc
      121 ggcgacgtcg gccgcgccgg gcgccgtgtc gccacgcagc gcggcggcaa agccagcctg
      181 gatctcaggc agcgagcgca tgggccgcct ccaggcgtcc ttgcgcccga tccgcctcac
      241 ccaccagaac cgacagctcc ggcaggttgg tgtcccattc gatgagggtg ggcacggggc
      301 catggcgccg cagcgccagt tcggacagcg tccagaccgg ctccggaacc tgcgagccat
      361 ggtcgtccag caggatctcg cgcccgtcca cgattcttcg cgcgtggcct gcgaggtgga
      421 tttcctgcac cgcgccggca ggcaggccgt tgatcaacgc ggtcgcgtcc aatccgacgt
      481 tgaccgagtt gacataggcg ttgttgacgt cgagcagcag gccgcagccg gagcgctcga
      541 cgacggccgc gaggaactcc cactccgtca gcctcgaaca cttgtagctg aggtagcagg
      601 acaggttctc gatcaggatc tggcggccca gcaggtcctg cgcctgggcg acacggtcgg
      661 ccaggtgtgc cgcggactct tccgtatagg gcaggggaag caggtcgttg aagtgctcgc
      721 cgtcatggct ggcccagcag gcgtgctcgg agaccagcga cggcgcgaag cgctcgatgg
      781 cgcgacgcag cttcgcgatg tgcgaccggt cgaggggatc cgcggagccc agccccatgc
      841 cgaccccgtg caggctgagc gggtaaagct cccgggcccg ctcgaggtcg cgcacgtgcg
      901 ggccgccgtc gtggaagtaa ttctcggtgt ggacctcgat ccagccgacg cgcggccgct
      961 cgcgcaggag cgcctcgtgg tgtgcggagc gcaagccgat cccggcagct gccgggatcg
     1021 gcccgctcga gttcgtttcg gggtccgaac tcatccgtcg ctcacatctt cttggcgggc
     1081 tcggtcttgc cgcccatcgt ctcgcactgg cccgcgggga cgaacttcca ctctgtcggg
     1141 ctgttgtcga ccttggactg gccggcacag gagtggccat tggccgcgca atcgttctgg
     1201 ccggccttgg cgatgccgta gcacttttcc ttgccggcct tgtcgcctgc gacggccgtc
     1261 gcggtcacgc ccatgctgat caggctgccg acagccgccg ccagggccag gttcctcata
     1321 ttcatcatcg atggttctcc gttatggatg gactcaaggg actgcatcat acaggcctga
     1381 ccggggggtt ctccccggct tggcgacctg tactgtagta gaccgcgccg gcgcggaaaa
     1441 tatttccgga tggggcagcc aaccggaatt gcgggcgatc gcgccgctcc ggccgaactg
     1501 cgaccgggaa atgaagcggt tttgctcccg ctcagttgag ggcaggatcg gcgatgtgcc
     1561 cgtagaactg gtcgggcagc catagcgcga tctgcgggaa caggatcacc agcgtcagcc
     1621 cgagggtcat gatgaggacg aacgggatga tggagcggta gatgtccatc agggagatct
     1681 cgggtggggc catcgcgcgc atcaggaaca ggttgtagcc gaacggcggc gtcatgtagg
     1741 cgatctggca ggtgatcgtg tacagcacgc cgtaccagat cgggttgaag ccgagcgcga
     1801 tgatcagcgg cacgtagagc ggcgccacga tgatgagcat cgcggtgtcg tccaggaaca
     1861 tgcccatcag gatgtaggtg agctgcatca gcaccagcac ctgccagggc tccagccccc
     1921 acttctcgag gaacagcgcc tcgatcgccc tgaccgcacc gaggccgtcg aagacggccc
     1981 cgaaggccag cgccgccagg atcacccaca tgaacatgca gctcacggcc agggtcttct
     2041 tcagcgtggc ggtcatgacc tcgcgcgtga tgcggccctt gaaccatgcg gccagggtcg
     2101 cggccgccgc gccggccgcc gagctttcca cgaggctggt gacgcccatc aggaacaggc
     2161 cggtcatcga gaagatgatc accagcggca ggatgccggc gcgcagcagg cgcatcttgt
     2221 ccacccacga gtactggtcg cgttcctcct tggccagcgg cggcccgagg tgcggctgga
     2281 acttgcagcg gatgaggata tatgcgacga acagcgccgc gagcagcagt ccggggaaca
     2341 cgcccgccag ccacagctgg ccgaccggct gccgggcgat catcccgtac agcaccagca
     2401 ccacgctcgg cgggatcatg atgccgagcg agctgcccgc ctggatgacg ccggtcacca
     2461 tgaccttgtc gtaattgcgt ttcagcagct cgggcagcgc aacgctggcg ccgatggcca
     2521 tgccggccac cgacaggccg ttcatcgccg agatcgccac catcagcagc acggtgccga
     2581 cggcgagccc acccgggagc gggccggccc agacatggaa catcttgtac aggtcgctgg
     2641 cgatccccga ctccgacagc atgtaaccca tgtagatgaa catgggcagt gtcagcatcg
     2701 ggtaccagtt gagcagcgtg atcgtggagg cgaagggcat ttccgcgccg cccttacccc
     2761 ataacgcgag ggcgaacacg gtcgcgacga agccgatggc gccgaacacc cgctggccgg
     2821 tgaccagcat cagcatcatc atggcgaaca tcagcagggc gatcagctcg tagttcacgc
     2881 gatctccctc cccatgaacc gcgcgaggtc cttgaagaag aacgagaacg cctgcagcag
     2941 catgagcgct atgccgacgg tcatgaccat cttgatgggc cacatcagcg gggcccaggc
     3001 ggagtaattg gtctggccgt acttgagcgc gtaggccgtg ctcgaccagc cgccgtagat
     3061 catgtagatc aggtagaaga cgatgcagag cgccgtgagc gaatcccaga acgcgcgcgt
     3121 gcgcggctgc caccggtcat agaacacgtc catgcggacg tgcgcctcca gctgcatcga
     3181 gtagccgcca ccgagcaggt agtaggcggc catcaggaac tgggccatct cgatgatcca
     3241 caggaaggag gcgttgaaga cgtagcgcga gatggagccg aacagcagca cgcccatcat
     3301 cacgaagatc atgaacagca tgaccgtgcc gacacccttg ttcagggcgt cgacgtagcg
     3361 cacgtaggcc cggatgggcc atggcatctt gctgacggtc gagttcacct gccgctcaga
     3421 ccccgctttt tcctcgtacc accccagccg cacggccggc gccggtgccg gagattaccg
     3481 ccaatgtgaa ggataccgcc aacacgaagg gtccttcccc ggcgttcccg tgaacgaccg
     3541 gggaaggacc ggacgacgtg cccctcaagg cccgaccgga tcaggaatag cggtacggag
     3601 ggcccgcctt cttcatcagg tcgttgtagt ccttgaagat ctggatcacg cgcgcacagc
     3661 gcgggctggt cgtagcgatc tcgtcccaga acttcagcgc ctcggcctcg accgtcgccc
     3721 actcctcggc ggggatggtc gtcagcttca tcttctcgcc cttgacgcgc aggtgcgcct
     3781 cgccgcccca gtaccagtac tggcggtagt agtgcgagct gtccatgcac agcttgaaca
     3841 gcgtctgcag gtgctcgggg accttgttcc acgcttcggt gttggcgaag taggaaccga
     3901 tccacgcgcc ggagatgttg ttggtgagga agtagttggt gacgtccgcc cagcc
//
LOCUS       DBNHOX010000745         1629 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_831748, whole genome shotgun sequence.
ACCESSION   DBNHOX010000745 DBNHOX010000000
VERSION     DBNHOX010000745.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_831748"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="AC16UZ_16165"
     CDS             complement(<1..1453)
                     /locus_tag="AC16UZ_16165"
                     /inference="COORDINATES: protein motif:HMM:NF017569.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR6248274.1"
                     /translation="MDRTLTNFIRALRNSEVRISTAETLDAFNTVELVGYRDRAALKR
                     SLALVLPKTADEKETFEQCFDAYFSFTDIQGSQGAAGEAERASDAETTAGGEEGGEGG
                     GAQPGEARPGDAAGSAKKKQKPGASADAEADEDEDLGAGAMSKPQSTLGQLLMANSKV
                     ELSVATAAAGEAVDVHDIEVFTQKGLYTRRIMEEMGLADLNREISELRQRPAVPDRRL
                     GQELTRRRDWLREQVRDYVEKQFLLHADASGKRLREELLRKVKLSNVEQRSFRLIQEI
                     VFRMAKKLAALHSRRKKVFKRGQINIPRTLRHNMAYDGAIFDLRWKSVKIDRPKVFAI
                     CDVSGSVANYARFMLMFLYSLEEVMPKVRSFAFSSDLAEVTELFERNAIEDAIAKTIR
                     DYSGGSTDYGQSLMDFKKLCLDEVDNRTTIIILGDARNNYGDPHTELLKELYDRCKRL
                     IWLNPEPRNAWGVGDSEMRRYAAYCHQVEECNSL"
BASE COUNT          249 a          523 c          524 g          333 t
ORIGIN      
        1 tgaggctgtt gcactcttct acctgatgac agtacgccgc gtaccggcgc atctccgagt
       61 cccctacgcc ccaggcgtta cgcggctcgg gattcagcca gatcagccgc ttgcagcgat
      121 cgtagagctc ctttaagagc tccgtgtgcg gatcgccgta attgttgcgg gcatcgccga
      181 ggatgatgat ggtcgtgcgg ttgtccacct cgtccaggca gagcttcttg aagtccatga
      241 gcgactggcc gtagtccgtc gagccgccac tgtagtcgcg gatggtcttg gcgatggcgt
      301 cctcgatggc gttgcgctcg aagagctcgg tcacctcggc cagatccgac gagaaggcga
      361 aggagcgcac cttgggcatg acctcctcca ggctgtacag gaacatcagc atgaagcgcg
      421 cgtagttggc gacagagccg gagacgtcgc agatcgcgaa aaccttgggc cggtcgatct
      481 tcacagattt ccagcgcagg tcgaagatgg ctccgtcgta ggccatgttg tggcgcaggg
      541 tgcgcggaat gttgatctgc cccctcttga acactttctt gcggcgcgaa tgcagagcgg
      601 ccagcttctt ggccatccgg aagacgattt cctggatgag gcggaagctg cgctgctcca
      661 cgttggacag ctttaccttg cgcagcagct cttcgcgaag ccgcttgcct gacgcgtcgg
      721 cgtgaaggag aaactgtttc tccacgtagt cgcgcacttg ctcgcgcagc cagtcccggc
      781 gccgggtgag ctcctggccg aggcggcgat caggcacggc cgggcgctgg cgcagctcgg
      841 aaatctcccg gttcagatcc gcaaggccca tctcctccat gatccggcgg gtgtagagcc
      901 ctttctgagt gaacacctcg atgtcgtgga cgtcgacggc ctctccggcc gccgccgttg
      961 ccacggacag ctcgaccttg gaattggcca tgagcagctg cccgagggtc gactgcggct
     1021 tgctcatcgc gccggcgccc agatcctcgt cctcgtctgc ctccgcgtcg gcgcttgcgc
     1081 ccggcttctg ttttttcttc gcgctgccag cggcatcacc cgggcgcgct tcgccgggct
     1141 gagcgccgcc gccttcaccg ccttcctcgc cgcctgcggt ggtctcggcg tcggaggcgc
     1201 gctcagcctc gccggccgct ccctggctgc cttggatgtc ggtgaaggag aaatacgcat
     1261 cgaagcactg ctcgaacgtc tccttctcgt ccgccgtctt gggtagcacc agcgcaagcg
     1321 agcgcttgag cgcggcccga tcccgatagc ccacgagctc cacggtgttg aaggcgtcga
     1381 gcgtctccgc cgtggagatg cgcacctcgg aattgcgcag cgcgcggatg aagttggtca
     1441 gggtccggtc catccggact tatttgagcg ccttgctggt cagcgtggtc acttccgcgt
     1501 ccacgttgtc gatgtcctcc tggaccttga ggatcacgtt gagggtgtcc cggaccagct
     1561 tggggtccag cgactcggtg tgcaggagca gcagcgtgcg cgcccagtcg atggtctcgc
     1621 tgatggccg
//
LOCUS       DBNHOX010000746         2745 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1731866, whole genome shotgun sequence.
ACCESSION   DBNHOX010000746 DBNHOX010000000
VERSION     DBNHOX010000746.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1731866"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="AC16UZ_16170"
     CDS             <1..475
                     /locus_tag="AC16UZ_16170"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6248275.1"
                     /translation="PESAGWRLLRVMPLHQMVCETPPGQPEPFDELTPSDAPEMVALA
                     ELTEPGPFLQRTIEMGRYVGVRSGGRLVAMAGERMKLPGCTEVSGVCTHPDARGRGLA
                     LRMVLNVLAECFARGDRAFLHVVAGSPSETTAIGIYERLGFEHRRPIYAHVLLRE"
     gene            complement(543..971)
                     /locus_tag="AC16UZ_16175"
     CDS             complement(543..971)
                     /locus_tag="AC16UZ_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007947151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6248276.1"
                     /translation="MSETHKGACFCGAVQIEVTGEPNMMGYCHCTDCAAWAGAPVNAF
                     SLWPPENVQVTAGEEKLGTYAKTEGSQRKFCTQCGGHVFTDHPGMGLIDVYLNVVPGK
                     AHTGAMHVFYGEKTMSVPDGLPKYRDLPKEFGGSGETIAE"
     gene            complement(1006..1770)
                     /locus_tag="AC16UZ_16180"
     CDS             complement(1006..1770)
                     /locus_tag="AC16UZ_16180"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6248277.1"
                     /translation="MSNPTQPLKLYGVPFSQPVRAVMWLMLHKRMPFEMVLTNPGTSG
                     ENGSRHPDYLAKNPGGTIPTIEEPDTGFTLGEAHAILCYLCNKYGWSDMYPAEPQERA
                     RVDWYLHYHHRNVREASTGLVAPKIRKDLNIPETVQAAAQATLTRALQAIETGWLGKS
                     AFLAGDAVTIADFAAYVEIGQLQPGFTNVYDFNPFPNVRRWMDSLKTFDSHDDVHVVL
                     AELGDISAEPPSMEAIKNANISALRTLKARLAEMSG"
     gene            1971..2660
                     /locus_tag="AC16UZ_16185"
     CDS             1971..2660
                     /locus_tag="AC16UZ_16185"
                     /inference="COORDINATES: protein motif:HMM:NF016705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOUL family heme-binding protein"
                     /protein_id="HIR6248278.1"
                     /translation="MLKVLMIAAGVLVAVVVAGIVAWWYWGTTLEQPEYAVISKGDGY
                     EVRRYEGYLVAQTRVSGEFDQAGTRAFPVLAGYIFGNNVERREMAMTTPVVSYDADTS
                     SGSTMAMTAPVFSEPAGAEDEASVDPSQEKLWTYQFVMERKYSEASLPAPVDPRVEIK
                     RIPPRLVAARRFSGRWLEENVKAAETALLSSVRAGGLEPVGQVVLARYNDPFTPWFLR
                     RNEVLVEIAGD"
BASE COUNT          451 a          824 c          921 g          549 t
ORIGIN      
        1 ccccgaatcc gcgggctggc ggctgctgcg ggtcatgccg ctgcaccaga tggtctgcga
       61 gacgccgccg gggcagccgg agcccttcga cgagctgacg ccgtccgacg ctccggagat
      121 ggttgccctt gccgagctga ccgagcccgg gccgtttctg cagcggacca tcgagatggg
      181 gcgctatgtg ggcgtgcgca gcggcggccg gctggtcgcc atggctggcg agcgcatgaa
      241 actgcccggc tgcacggagg tcagcggcgt gtgtacccat cccgacgcgc gcggtcgggg
      301 gctggccttg cgaatggttc tgaacgtgct ggccgaatgc tttgcccgtg gcgatcgggc
      361 gtttctgcac gtggtggccg gcagcccgag cgagaccacc gccatcggaa tctacgagag
      421 gctcggcttc gaacaccgca ggccgatcta cgcccacgtt ctgctgcgcg agtaaaccgt
      481 gtctcaatcg gcggcccgtg cagaccggac cccgggtcct ccgctcaccg ctcgcggcgg
      541 tcctactcgg cgatggtttc gccggaaccg ccgaactctt ttggaagatc ccggtatttc
      601 ggcaaaccgt ccggtaccga catcgtcttc tcgccgtaga agacgtgcat ggcgcccgtg
      661 tgggccttac cgggcacgac gttgagataa acgtcgatta gtcccatccc gggatggtcg
      721 gtgaacacgt ggccgccgca ttgcgtgcag aacttgcgct ggctgccttc ggttttggca
      781 taggtgccca gcttctcctc gcccgccgtt acctggacgt tttccggcgg ccacaggctg
      841 aacgcgttca ccggggcccc ggcccaggcc gcgcaatccg tgcagtggca gtaacccatc
      901 atgttgggct ctccggtgac ctcgatctgg accgctccgc agaagcaggc gcccttgtga
      961 gtttcgctca tgattattcc tttgctcgtg ttcaggattg ccgggtcatc cgctcatctc
     1021 cgccaggcgc gccttgagcg ttctcagagc ggagatgttg gcgttcttga ttgcctccat
     1081 ggaaggcggc tcggcgctga tgtcgcccag ctcggcgagc acgacatgga cgtcgtcgtg
     1141 actgtcgaag gttttgagcg agtccatcca gcggcgaacg tttggaaacg ggttgaaatc
     1201 atagacgttg gtgaagccgg gctgcagttg gccgatctcc acgtaggcgg cgaagtcggc
     1261 gatggtgacc gcatcgccgg cgagaaatgc ggactttccg agccagccgg tctcgatggc
     1321 ctgcagcgcc cgggtcaggg tggcctgggc ggccgcctgg acggtctccg gaatgttcag
     1381 atctttccgg atcttcggcg ccaccaaccc ggtcgatgcc tctctgacgt tgcgatggtg
     1441 atagtgcagg taccagtcca cgcgggcgcg ctcctgaggc tcggctggat acatatccga
     1501 ccagccgtat ttgttgcaca gatagcagag gatggcgtgc gcttcgccca gggtgaagcc
     1561 ggtatcgggc tcctcgatgg tcgggatggt gccgcccgga ttcttggcca gatagtccgg
     1621 gtgacgactg ccgttctcgc ccgatgtgcc ggggttggtg agtaccattt cgaacggcat
     1681 acgtttgtga agcatcagcc acatgacggc gcggacgggt tgagagaacg gcacgccgta
     1741 gagtttcagt ggttgggtgg ggttgctcat cattcctccc tgatcggacg gccaagctat
     1801 catgccttct gtccggtgat aagctcccga ggctgcaccg cagtcccgtc gcattccggc
     1861 gcgggggatt cacgtgcatt tcaccgtctc gcgagcatgg ttgaatacag gttcgaggag
     1921 aaaggaaaga ccatgaccgc catgctcgtc gcccgaacga agctcccatc atgttgaagg
     1981 tcctgatgat tgcagcaggc gtgctcgttg ccgtcgtcgt ggccgggatc gttgcctggt
     2041 ggtactgggg cacgactctt gaacagcccg aatacgcggt gatctcgaaa ggtgatggct
     2101 acgaagttcg tcgctacgaa ggctacctcg tcgcgcagac gcgggtgtcc ggggagttcg
     2161 accaggcagg tacccgggcg ttccccgtgc tagcggggta catcttcggc aacaacgtgg
     2221 agcgtcggga gatggccatg accacgccgg tggtgagcta cgatgccgac acgtcttcag
     2281 gcagcacaat ggccatgacc gcgcccgttt tcagcgagcc tgccggagca gaagacgagg
     2341 catcggttga tccatcgcaa gagaagctgt ggacgtacca gttcgtgatg gaacgcaagt
     2401 actccgaggc gtcactgccc gcgcctgtgg atccgcgcgt tgagatcaag cggattccgc
     2461 cgcggttggt ggcggcgcgc cgcttctcgg gccgatggct cgaggagaat gtgaaggctg
     2521 cggagacggc gctgctgagt tcggtgcgcg ccggcggcct cgagcccgta gggcaagtgg
     2581 tcctcgcccg ctataacgac cccttcacgc cgtggtttct gcgacgcaac gaggtgctcg
     2641 tggagatcgc tggcgattga cagccgccat ggtcgtgggg tctgtctcgg cgtaggattg
     2701 ttgcctctgg ttggagaaac gtcatgtcaa accacacagc ctgcg
//
LOCUS       DBNHOX010000747         2615 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_382174, whole genome shotgun sequence.
ACCESSION   DBNHOX010000747 DBNHOX010000000
VERSION     DBNHOX010000747.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_382174"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="AC16UZ_16190"
     CDS             <1..660
                     /locus_tag="AC16UZ_16190"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR6248279.1"
                     /translation="ILTLLFFTPLFTYLPHAALAAIVIVSVTSLLDFRSFRTHWRIHR
                     EDSVTQMVTLASVLLFGVEAGLLTGVTLSIAFFIRQSSRPNVTLVGRMGDTEQFRSAR
                     RYPVKTFEHVAAVRVDENLYFGNANQVENKLLKVVHRRPATKHLVLVCSAINMIDVSG
                     LEMLYRINQNLTDMGITLHLSEVKGPVMEQLAATDFISALAGTVYFTTDQAMRDLAPR
                     T"
     gene            672..1565
                     /locus_tag="AC16UZ_16195"
     CDS             672..1565
                     /locus_tag="AC16UZ_16195"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6248280.1"
                     /translation="MTDELDTADWECVLEGPRGAMTEAGFVLTAVAIEHRIELCGRIS
                     RLLVPPSQLAQAMAELESYQQENRPLPGSVYSVPTVDGGWAGVLGYLMVIWLQPSLEA
                     NQAFGWDWREAGHLQAGLVAAGEWWRCITALTLHGDLGHIVANSLFGSLFGLYVGRYL
                     GSGFGWLLILASGALGNAMNAWIQPDAFSSIGASTASFGALGVSAAFVWRRGYYRNRD
                     WRRSLAPIFAGIALLAFTGTGGENTDVVAHFTGFAAGALLGAAAAAFDIRRLGVSGQY
                     LAGAAAITLVVTAWSFAGTVG"
     gene            complement(1566..2144)
                     /locus_tag="AC16UZ_16200"
     CDS             complement(1566..2144)
                     /locus_tag="AC16UZ_16200"
                     /inference="COORDINATES: protein motif:HMM:NF016270.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HIR6248281.1"
                     /translation="MSHITTTFLRGLGVVLPLSLTIWFVVWLAVTTESLLRSVFLVIL
                     PEGLYLPGLGLIFGIVMVYGVGVLAQIFFLQSLWRHARNLLDRIPLVKTVYTAISDFY
                     QFFSSGPADGAARVVSVDVSDDVSVIGFVTGPSPSFMDQGGSRRIAVYFPMSYQIGGY
                     TLMVPEDRVSPLDIGVEDAMRVILTASVRSRR"
     gene            2281..>2615
                     /locus_tag="AC16UZ_16205"
     CDS             2281..>2615
                     /locus_tag="AC16UZ_16205"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6248282.1"
                     /translation="MKLGLALGYSGAYLNIPLKKVQLAERLGYDSVWTAEAYGNDALT
                     PAAWVLANTERIKVGTAIIQMPARTPAMAAMSAMTLDHLSGGRFILGLGASGPQVVEG
                     WHGVPYGKP"
BASE COUNT          467 a          865 c          841 g          442 t
ORIGIN      
        1 attctcacgc tgctgttctt caccccgctg ttcacctacc tgcctcatgc ggcgctcgcc
       61 gccatcgtca tcgtatccgt aacgagcctg ctcgacttcc gcagcttccg cacacactgg
      121 cggatacacc gtgaggacag cgtgacccag atggtcaccc tggcctcggt gctgcttttc
      181 ggcgtggaag ccgggctcct cacgggcgtc actctttcca tcgcgttctt catccgtcag
      241 tccagccgtc ccaacgtgac gctggttggt cggatggggg acacggagca gttccgctcc
      301 gcacgacgct accccgtcaa aacgttcgag cacgtagcgg ccgtgcgcgt tgacgagaac
      361 ctctacttcg ggaacgccaa ccaggtggaa aacaagctgc tgaaagtcgt gcatcgaagg
      421 cccgccacca agcacctggt actggtgtgc agcgccatca acatgatcga cgtcagcggc
      481 ctggaaatgc tctaccgcat caaccagaac ctgaccgaca tgggaatcac gctgcacctt
      541 tccgaggtga aaggccccgt gatggagcag ctggccgcca ccgacttcat ctcggcgctg
      601 gcgggcaccg tctacttcac caccgaccag gccatgcgcg acctggcgcc caggacctga
      661 gcgctagcgg cgtgaccgac gaactcgaca ccgcagactg ggaatgcgtt ctcgaagggc
      721 cccggggagc catgacggag gcaggattcg ttctgacagc ggtggccatc gagcaccgca
      781 tcgagctctg cggacgcatt tccaggttac tggtcccccc atcgcagctg gcccaggcga
      841 tggcggagct cgagagttac cagcaggaaa accgaccgtt gcctggctcc gtatacagcg
      901 tgccgacggt ggacggcggc tgggcgggcg tgctcggcta tctgatggtg atctggctgc
      961 agccgtccct ggaggcaaac caggccttcg gctgggattg gcgcgaagcc ggacaccttc
     1021 aggcaggcct cgtcgccgcc ggcgagtggt ggcgctgcat cactgcgctc accctgcacg
     1081 gcgacctggg ccacatcgta gccaactcgc tgttcggctc gctgttcggc ctgtacgtcg
     1141 gccggtacct cggctccggc ttcggctggc ttttgatcct cgccagcggc gcgctcggca
     1201 acgccatgaa cgcgtggatc cagccggacg cgttcagctc catcggcgca tccaccgcga
     1261 gcttcggcgc cctgggcgtg agcgcggctt tcgtgtggcg ccgcggctat taccgcaacc
     1321 gcgactggcg ccgcagcctt gcgccgatat tcgcgggtat agccctgctg gcgttcaccg
     1381 gcaccggggg ggagaacacc gacgtggtcg cgcacttcac cggctttgcc gcgggcgccc
     1441 tcctcggcgc cgcagccgcc gcgttcgaca tccgtcgtct gggcgtctcc gggcagtatc
     1501 ttgcgggggc cgcggccatc accctcgtcg tgacggcctg gtcgttcgcc ggcaccgtcg
     1561 gttgatcacc gcctgctgcg cacggaggca gtcagaatca cccgcatggc gtcctctacg
     1621 ccgatgtcca acggcgacac ccgatcttcc gggaccatga gcgtgtagcc gccaatctgg
     1681 taactcatgg gaaagtagac ggcgatccgc cggctgccgc cttggtccat gaaggatgga
     1741 gaggggccgg tcacgaagcc aatgaccgag acgtcatctg agacgtccac gctcacgacc
     1801 ctcgccgcgc cgtcggccgg cccgctggag aagaactggt aaaagtcgct gattgccgtg
     1861 tacaccgttt tgaccagcgg aattcggtcc aggagattcc gggcgtgccg ccataggctc
     1921 tgcaggaaaa agatctgagc gagcacgccg accccgtaga ccatcacgat gccgaatatg
     1981 aggcccaggc ccggcaggta gaggccctcc ggcaggatga ccaggaacac cgagcgcagc
     2041 agggactccg tggtcacggc cagccagacg acgaaccaga tggtcaggga aaggggcagc
     2101 acgacgccga gaccgcgcaa aaaggtagtg gtgatgtggc tcatgacgat acgaccttga
     2161 agttgatgta gcgaagtcta gccgttcgcg cgacgccgcg ttcaaccgcg ccgatggtcg
     2221 gcccgacggg cggccgccac ttgagttaag ctccaggcaa aagtcgcgca ggagctgacg
     2281 atgaaactgg gactggcttt agggtattcg ggcgcgtacc tgaacatccc attgaagaag
     2341 gttcagctgg ccgagcggct gggctacgac tccgtctgga cggccgaggc ctacggcaac
     2401 gacgcgctca cgcccgcggc gtgggtgctc gcgaacacgg agaggatcaa ggtcggcacc
     2461 gcgatcatcc agatgcccgc gcgcacgccc gcgatggcgg cgatgagcgc gatgacgctc
     2521 gaccacctgt cgggcggccg cttcatcctc gggctcggag cgtcgggtcc gcaggtggtc
     2581 gagggttggc acggcgtgcc ctacggcaag ccgtt
//
LOCUS       DBNHOX010000748         4789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1088896, whole genome shotgun sequence.
ACCESSION   DBNHOX010000748 DBNHOX010000000
VERSION     DBNHOX010000748.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1088896"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="AC16UZ_16210"
     CDS             <1..962
                     /locus_tag="AC16UZ_16210"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6248283.1"
                     /translation="EHREANMSDPEAAVMSIGAVTKATGISTHTLRKWESRYGVIEPL
                     RTPTGRRVFTQSQVDRLILLRDLLAQGHQIGRLSTLADDELEALARATPARSRVPEFG
                     LVTVVGPLISAVLSREALGPAPFRLVTRPATEWLAGGEEGSDQEAQALVVELSTLSEE
                     SCGRLLELRRGVYQRVIVVYGFAARTTLQMLLDGGIVCLKAPVDGQEVLRALAQSEAP
                     GVVAALADPTIPAPRFSPDSIARLAAMAPAIRCECPNHIAQLLTDISAFEEYSKLCEQ
                     GDPADRVLHARLRLIAANARALFEEAMENVARAEGLELEELDG"
     gene            complement(982..2088)
                     /gene="modC"
                     /locus_tag="AC16UZ_16215"
     CDS             complement(982..2088)
                     /gene="modC"
                     /locus_tag="AC16UZ_16215"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HIR6248284.1"
                     /translation="MSVDELDIEITASLPGFDLRLKRTLPLQGVTAVFGPSGSGKSTL
                     LRVLAGFIRPASGRVSCGGDVWFDKAANVHLPPHRRPVGMMFQDARLFPHLNVAGNLD
                     FAVRRQRRPGNGIEQPEVVAALDLGPLLTRQVDSLSGGERQRVALGRTLLASPRLLLL
                     DEPLAALDRERKADILPYLDQIQRGFGIPTLYVSHDVDEVAQLCDRVLVLAGGSERAF
                     GATADIVERLDLEPLMGRFEAGVLLEGTVTGHDPRLHVTHVDVGGDVLTLPLLSGVAE
                     GHAVRVRIRARDVALATTQPEGLSIRNVLPGRISRLLIEEASGTAEAFVELRGAHLRS
                     RLTLAAVEDLGLREGMPVYALIKSISFESSAPGG"
     gene            complement(2078..2749)
                     /gene="modB"
                     /locus_tag="AC16UZ_16220"
     CDS             complement(2078..2749)
                     /gene="modB"
                     /locus_tag="AC16UZ_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR6248285.1"
                     /translation="MPDLAPLWLTLHLATATTLILLVIGTPIAWWLARTRSPLRAPVE
                     ALTALPLVLPPTVLGFYLLVMLSPTSPFGSFWVQVTGEALTFSFAGLVVASVIYSLPF
                     MVQPLTNAFSAIGPGPMEAAASLGAGPFDAFRCVAAPLAKRGFVTAAALSFAHTVGEF
                     GVVLMVGGNIPGETKVISIAIYEHVETLRYAEAHLLSAGLVAFSFVVLLTVYGLNRRS
                     PLNVG"
     gene            complement(2750..3514)
                     /gene="modA"
                     /locus_tag="AC16UZ_16225"
     CDS             complement(2750..3514)
                     /gene="modA"
                     /locus_tag="AC16UZ_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6248286.1"
                     /translation="MSSARKRSRLLQLLATCWLLAGAGNAWAERALAAVASNFVVPAE
                     LLAAEFERATGHTVSIAAGATGKLYAQVVQGAPFDLLLAADAWRPERLELEGRAVPGS
                     RFTYAVGLLTLWSPRPDAFGEDPVGYLRAGRLRRLALANPDLAPYGTAARQVLEHLGL
                     QAQLADKMVFAENIGQAHAMVDTGNADAGFLARAQVIGRPGSHWNVPVGMHLPIEQQA
                     VLLRRAADNAAATGFLGFLRTEAARGAMATAGYGLP"
     gene            complement(3614..>4789)
                     /locus_tag="AC16UZ_16230"
     CDS             complement(3614..>4789)
                     /locus_tag="AC16UZ_16230"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6248287.1"
                     /translation="AFAQQLARVMDRAEEDPEWALERLAEMMRANRNRPTELRYAVRQ
                     RAAMLMQTERLDLAAEEMLAAVTGKDPGYAPELRFLLGQVWLLKDDARTALGHLELWA
                     RHADPLDPAGLFLVGYAHLQLDEFEPAAKALETAITTSIKPRPQWFEILAYVYTRLDR
                     PADAIGLLETLIASQPQQSRWWRQLGSIYLLIDDVPRGTAGLTVLAELEPLGLSDSRS
                     LARLFASLDMPADAAYVLDAAMTRARTAAELPGAPSEPDGSEKPEVPQELPGYEDVML
                     LAELWIMAREFDAAVGTLKTASAMRDDGEAQTLLGQLYLQREDYPQADRHLDAALAAY
                     GEEPPPRLIYLKAVVALNRGDDEAASRALKRISGDEAFAQRVARLQRYIDSRRQRGA"
BASE COUNT          774 a         1601 c         1686 g          728 t
ORIGIN      
        1 ctgagcatcg ggaggcaaac atgtcagatc cggaagcggc agtaatgtcc ataggggccg
       61 tcaccaaggc gacgggcatc tccacccaca ccctgcggaa gtgggaaagc cgctacggcg
      121 tcatcgagcc tctgcgcact cccaccggcc ggcgggtctt cacgcagtca caggtcgacc
      181 ggctcatcct cctgcgggac ctgctggccc agggccacca gatcgggcga ttgagcaccc
      241 tggcggatga tgagctggag gcgctggccc gcgccacgcc ggctcgcagc cgcgtccccg
      301 agttcggcct ggttaccgtc gtgggtccgt tgatctcggc ggtgctttcc cgcgaggcgc
      361 tgggaccggc accgttccgc ctggtcaccc ggcctgcgac cgaatggttg gcaggcggtg
      421 aggaaggctc ggatcaggag gctcaggctc tggtcgtgga gctgtcgacg ttgagcgaag
      481 aatcgtgcgg gaggcttctc gagctccggc gaggcgtgta ccagcgcgtg atcgtcgtct
      541 acggttttgc agcccgcacc accctgcaga tgttgctcga tgggggcatc gtctgcctca
      601 aggcgcccgt tgacggccag gaggtgctgc gggcccttgc ccagagcgaa gcgcccggag
      661 tggtggcggc cctcgccgac ccgacgattc ccgcacctcg attctcgccg gacagcattg
      721 cccggctcgc tgccatggcg cccgccatcc gctgtgagtg ccccaatcac atcgcccagc
      781 tgctcacgga catcagcgcc ttcgaggagt acagcaagct ctgcgagcag ggcgacccgg
      841 cagaccgggt gctgcatgcc cggctgagac tcatcgctgc gaacgcccgt gcgctcttcg
      901 aggaagccat ggagaacgtc gcgagagccg aaggcctgga gctcgaagag ctggacggct
      961 gaagagctgc agagctgagt ttcagccgcc gggcgcgctg ctctcgaagc tgatgctctt
     1021 gatgagcgca tagacgggca tgccttcccg aagccccaga tcctccacgg ccgccagcgt
     1081 gagccgcgat cggagatggg ctcccctgag ctcgacgaaa gcctcggcgg tgcccgacgc
     1141 ttcctcgatg agcagccggg atatgcgacc cggcagcacg ttgcggatgc tcagaccctc
     1201 tggctgggtg gtggccaggg cgacgtcccg ggcccgtatg cgcacgcgga ccgcgtggcc
     1261 ttccgccacg ccgctgagca gcggaagggt gaggacgtcg ccgcccacgt cgacgtgggt
     1321 gacgtgcagg cgagggtcgt gcccggtcac cgtgccctcg agcagaacgc cggcctcgaa
     1381 gcggcccatc agcggttcca ggtcgagccg ctcgacgatg tcggccgttg cgccgaacgc
     1441 ccgctcgctg ccgccggcca gaacgagcac gcggtcgcac agctgagcca cctcgtcgac
     1501 gtcgtggctc acgtagagcg tcgggatgcc gaagccccgc tggatctgat cgaggtacgg
     1561 gaggatgtcg gctttgcgtt cgcggtccag cgctgccaga ggctcgtcga gcagcagcag
     1621 ccggggcgag gcgagcaggg tgcgccccag cgccacccgc tggcgctcgc ccccggagag
     1681 cgaatcgacc tggcgcgtga ggaggggacc cagatccagc gcggccacca cctcgggctg
     1741 ctcgatgccg ttgcctggcc gccgctgacg tcgcacggcg aagtccaggt tcccggccac
     1801 gttcaggtgc gggaatagcc tcgcgtcctg aaacatcatg cccaccgggc gccggtgtgg
     1861 cgggaggtgc acgttggcgg ccttgtcgaa ccagacatcc ccgccgcagg agaccctgcc
     1921 ggacgcgggc ctgatgaagc cggccagcac tctgagcagg gtgctcttgc cgctgccgct
     1981 tggtccgaac accgcggtga ccccctgtag cggcagcgtc cgcttcaggc gcaggtcgaa
     2041 accgggcagc gatgccgtga tctcgatgtc cagctcgtca accgacattg agcggggacc
     2101 tccggtttag accgtaaacg gtcaggagaa cgacgaagct gaaggccacc aacccggcgg
     2161 agagcagatg ggcctcggcg taccgcaggg tctccacgtg ctcgtagatg gcgatggaga
     2221 tcaccttggt ctcgcccggg atgttgccgc ccaccatcag gaccacgccg aactccccga
     2281 cggtgtgggc gaaagacaag gcggcggccg tgacgaaccc ccgcttggcc agaggcgcgg
     2341 cgacgcaacg gaacgcgtcg aacgggccgg cgcccaggct ggcggcggcc tccattggcc
     2401 ccgggccgat ggcggaaaag gcgttcgtca gcggctggac catgaagggc agcgagtaga
     2461 tgaccgatgc caccacgagt cctgcgaacg agaaggtcag cgcctcaccg gttacctgta
     2521 cccagaagct tccgaacggc gaggtcgggc tgagcatgac gagaaggtaa aagccgagca
     2581 cggtaggtgg cagcaccagt gggagcgcgg tgagagcttc cacgggggct cgcagcggcg
     2641 agcgtgtgcg cgccagccac caggcgatgg gcgtgccgat gaccagcagg atgagcgtgg
     2701 tcgccgtcgc cagatgcaga gtcagccaca gtggcgcgag gtcgggcatc tacggcaatc
     2761 cgtacccggc cgtggccatc gcgccgcggg ctgcttccgt gcgcagaaac ccgaggaacc
     2821 ccgttgccgc cgcattgtcc gccgcccgcc ggagaagcac ggcctgctgc tcgattggca
     2881 ggtgcatgcc cacgggcacg ttccagtggc tgcccgggcg gccgatcacc tgggcgcggg
     2941 caagaaaacc ggcatcggcg ttgccggtat cgaccatggc gtgggcctgg ccgatgtttt
     3001 ccgcgaacac cattttatcg gccagctgag cctgcaggcc gaggtgctcc agcacctggc
     3061 gcgcggcggt tccgtagggc gccagatccg ggttggcaag ggcgagccgg cgcagacggc
     3121 cggcgcgcag atagcctacc ggatcctcgc cgaaggcatc cgggcgtggg ctccagaggg
     3181 tgagcagacc cacggcatag gtgaatcgcg atccgggcac cgcgcgacct tcgagctcca
     3241 ggcgctcggg tcgccaggcg tcggcggcga gcagcaggtc gaagggcgct ccctgcacga
     3301 cctgagcata tagctttccg gtagcgccgg ccgctatgga aaccgtgtgc ccggtggcgc
     3361 gttcgaactc ggcggccagc agctcggcgg gcacgacgaa gttgctggct actgccgcca
     3421 gcgcccgctc ggcccaggcg ttgcccgccc cggcaagcag ccagcacgtg gccagcagct
     3481 gcagcaggcg gcttcgcttc cgagctgagc tcatggactc tccctggttc ccgatgggtg
     3541 ggttcatagc acattcggcc cggaataagg aggccgccgg gcgccattgc atgttgcggt
     3601 gaaggagaat gcttcaggcg ccgcgctgac gccggctgtc gatgtagcgc tgcagcctgg
     3661 cgacgcgctg ggcgaatgcc tcgtcaccgg atattcgctt cagggcccgg ctggccgcct
     3721 catcgtcgcc ccggttcagg gcaacaaccg ccttcagata gatcagtcgc ggtggaggct
     3781 cttcgccgta ggcggcgagc gccgcgtcga ggtgtctatc ggcttgcggg tagtcctccc
     3841 gctgcaggta tagctgcccg agcagcgtct gtgcctcgcc gtcgtcccgc atggcggaag
     3901 cggttttgag tgtccccacg gcggcgtcga actcccgggc catgatccac agctcagcca
     3961 gcagcatcac gtcctcgtag ccgggaagct cctgcggaac ctcgggcttc tccgagccgt
     4021 cgggttcgga cggcgctccc ggcagctcgg ccgccgtccg ggcgcgtgtc atggccgcat
     4081 ccaggacgta ggctgcatcg gcgggcatgt cgaggctggc gaacagcctc gccagactgc
     4141 gactgtcgct gagccccagg ggctccagct cggcaagcac ggtgaggccg gccgtgcccc
     4201 ttgggacgtc gtcgatcagc aggtagatcg aacccagctg gcgccaccag cggctctgct
     4261 ggggctgcga ggcgatgagc gtttccagaa ggccgatggc gtcggcggga cgatcgagcc
     4321 gggtgtagac gtaggcgagg atctcgaacc actggggcct cggcttgatc gaagtggtga
     4381 tcgccgtctc cagggctttc gccgcgggct cgaactcgtc cagctgcaga tgggcgtagc
     4441 cgaccaggaa cagacccgcc gggtccaggg gatcggcatg gcgcgcccag agctcgagat
     4501 ggcccagcgc cgttctggcg tcgtctttca ggagccagac ctggcccagc aggaaacgca
     4561 gctcgggggc gtagcccggg tctttgccgg tcaccgccgc cagcatctcc tcggcggcca
     4621 ggtccagacg ttcggtctgc atgagcatgg ccgcacgctg ccggacggcg taccgcagct
     4681 ccgtgggccg gttgcggttg gcgcgcatca tctcggccag acgctccagg gcccactcgg
     4741 ggtcctcctc cgcccggtcc atgacgcggg cgagctgctg ggcaaacgc
//
LOCUS       DBNHOX010000749         2498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1088958, whole genome shotgun sequence.
ACCESSION   DBNHOX010000749 DBNHOX010000000
VERSION     DBNHOX010000749.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1088958"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /gene="polA"
                     /locus_tag="AC16UZ_16235"
     CDS             <1..1536
                     /gene="polA"
                     /locus_tag="AC16UZ_16235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR6248288.1"
                     /translation="GQNLKYDMSVLQRYDVQLAGVGFDTMLESYVLNSTASRHNMDDL
                     AKNYLSRETIHYEDIAGKGAKQLTFNQVPVEQAVSYAGEDADITLQLHQTLWPRLEKE
                     PRLTEVFREIEMPLLSVLSRMERKGTLVDGALLEAHGRELAERMRGITEEAWTLAGEE
                     FNLDSPKQLQAILFDKLELPVLKKTPKGQPSTAEPVLVDLARDFDLPRVILEYRSMAK
                     LKSTYTDKLPLQINQRTGRIHTSYHQAVAATGRLSSSDPNLQNIPVRTPEGRRIRQAF
                     VAPPGKRIVAADYSQIELRIMAHLSADDGLRAAFAANRDIHRATAGEVFGVGVDAVTD
                     DQRRSAKAINFGLIYGMSAFGLARQLGISRTLAQEYIDRYFERYPGVLAYMDETRARA
                     RDAGYVETVFGRRLYLPEINARNMQRRQAAERAAINAPMQGTAADIIKKAMLAVDAWL
                     AGSELDAVMIMQVHDELVFEVADADLDHLLAGVRDHMQGAADLDVELVVDCGTGANWD
                     EAH"
     gene            complement(1533..2174)
                     /gene="yihA"
                     /locus_tag="AC16UZ_16240"
     CDS             complement(1533..2174)
                     /gene="yihA"
                     /locus_tag="AC16UZ_16240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR6248289.1"
                     /translation="MTETERTHKANKDRGSADRLTVQFISSAPSLRKCPRPDGPEIAL
                     AGRSNAGKSSVLNRITGSRTTAKVGKTPGRTRLLNFFAVEGGGRLVDLPGYGYAKAAR
                     SAQHEWQATVNDYLSNREALRAVVLVSDVRHPGQRFDMELLAWADASELPLLVLLNKA
                     DKLGRNAQAKALKAMQRAAEAMPLVDVILFSATSGLGNDAVVAWLREHLNAGA"
     gene            2281..>2498
                     /locus_tag="AC16UZ_16245"
     CDS             2281..>2498
                     /locus_tag="AC16UZ_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6248290.1"
                     /translation="MQIRWLPCASAIVVCLGLWAPGLAWAAGDPEAGAAQAVVCAACH
                     GQDGASGVDPSYPNLAGQNEKYLYNQLV"
BASE COUNT          422 a          803 c          828 g          445 t
ORIGIN      
        1 gggcagaacc tcaagtacga catgagcgtg cttcaacgct acgacgtgca gctcgccgga
       61 gtgggtttcg acaccatgct cgagtcctac gtgctcaaca gcaccgcttc ccgtcacaac
      121 atggacgacc tggcaaagaa ctatctctcc cgggagacca ttcactacga ggacatcgcc
      181 ggcaagggcg ccaagcagct gaccttcaat caggtacccg tggaacaggc cgtgtcgtac
      241 gccggggagg atgcggacat cacgctgcag ctgcaccaga ccctctggcc caggctcgag
      301 aaggagccgc gtctgacgga ggttttcagg gagatcgaga tgcccctgct gtccgtcctc
      361 tcccggatgg aacgcaaggg cacgctggtg gacggcgccc tcctcgaagc ccacggccgc
      421 gagctggccg agcggatgcg gggcatcacg gaggaggcct ggacgctcgc cggcgaggaa
      481 ttcaacctag actcacccaa acagctgcag gcgattcttt tcgacaagct cgagctgccc
      541 gtgctgaaga agacgccgaa aggtcagccc tccaccgccg agcccgttct ggtcgacctg
      601 gcccgggact tcgacctgcc gcgcgtcatc ctcgagtacc gcagcatggc caagctgaaa
      661 tcgacctata ccgacaagct gccgctgcag atcaatcagc gcacggggcg gattcacacc
      721 tcatatcatc aggcggtggc cgccacgggc cggctttcct cgtcggatcc gaacctgcag
      781 aacattccgg tgcgtacgcc cgaaggccga cgcatccggc aggcgttcgt ggcgccgccg
      841 ggcaaaagga tcgtagccgc cgactactct cagatcgagc tgcgcatcat ggcgcatctc
      901 tcggccgacg acggcctgcg ggcggctttc gcggcgaacc gggacatcca ccgagccacg
      961 gccggtgaag tattcggcgt cggcgtcgat gcggtcaccg atgatcagcg ccgcagcgcc
     1021 aaggccatca acttcgggct tatctacggg atgtccgcct ttggcctcgc gcgccagctg
     1081 ggcatttccc gaaccctggc ccaggaatac atcgaccgct acttcgagcg gtatcccggc
     1141 gtcctggcct acatggacga gacccgggcg cgggcacgcg atgcggggta cgtggaaacc
     1201 gtcttcgggc gccggctcta tctcccggag atcaacgccc gcaacatgca gcggcgtcag
     1261 gctgcggagc gcgccgcgat caacgcgccc atgcagggca ccgcggcaga tatcatcaag
     1321 aaggcgatgc tcgccgtgga tgcctggctg gcgggctcgg agctggatgc cgtgatgatc
     1381 atgcaggtcc atgacgagct ggtgttcgag gttgcggatg ctgacctgga ccatctgctg
     1441 gcgggcgtcc gtgatcacat gcagggcgct gccgacctgg atgtcgagct ggttgtggat
     1501 tgcggtaccg gggccaactg ggacgaggcg cactaggctc cggcgttgag gtgctcgcgc
     1561 agccaggcca ccaccgcgtc gttgccgagc ccgctcgttg cggagaacag gatgacgtcc
     1621 accagcggca tggcttcggc cgcgcgctgc atggctttca gcgccttggc ctgagcgttc
     1681 ctgcccagct tgtcggcttt gttcaagagg acgagcaagg gcagttccga cgcgtccgcc
     1741 cacgccagga gctccatgtc gaagcgctgc ccgggatgcc ggacgtcgct tacgagcacc
     1801 acggcccgga gcgcttcgcg gtttgacagg tagtcgttca cggtagcctg ccattcgtgc
     1861 tgtgcggacc gggcggcctt tgcgtaaccg tagcctggca gatccaccag gcgcccgccc
     1921 ccttccacgg caaagaagtt gagcagcctg gtccggcccg gcgtcttgcc cacttttgcc
     1981 gtggtgcggc ttccggtgat ccggttgagc acgctggact tgccggcgtt cgagcggccg
     2041 gcgagggcga tctcgggccc gtcgggccgg ggacatttgc gcagcgacgg ggcgctggag
     2101 atgaattgca cggtcaggcg gtccgcgctg ccgcggtcct tgttggcctt gtgggtcctt
     2161 tcggtctccg tcaccctgtt gccctggtgg tccttgaagt tgcattctgc cagctcagcg
     2221 gagttgttat ataatccgcg cgccccgctc gggggcgggt agtgtaaagg aatcagagcc
     2281 atgcagattc gttggttgcc gtgcgcttcg gccatcgttg tttgcctcgg gctgtgggcg
     2341 ccggggctgg cgtgggccgc gggtgatccc gaagcagggg ctgctcaggc cgtggtctgt
     2401 gccgcctgcc atgggcagga cggtgcgtcg ggtgtggatc ccagctaccc gaacctggcc
     2461 ggccagaacg agaagtatct atacaaccag ctcgtcat
//
LOCUS       DBNHOX010000750         2630 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_512367, whole genome shotgun sequence.
ACCESSION   DBNHOX010000750 DBNHOX010000000
VERSION     DBNHOX010000750.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_512367"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /gene="era"
                     /locus_tag="AC16UZ_16250"
     CDS             <1..582
                     /gene="era"
                     /locus_tag="AC16UZ_16250"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR6248291.1"
                     /translation="DEVVLAHVRKARAARFAVINKIDLIRNKNRLLPVIEHLQNLGCF
                     DQVIPVSALRDEGLDALRAEVASSLPQHPHMFPADQLTDQPERFLVAEIIREKLMRRY
                     GDEIPHRTAVVIEGYQEGERVTDIAADIYVERPGQKRIIIGKGGEKLREVGRDARQDI
                     EKLLDRKVMLRLWVKVKPGWTNDRTQLRRMGFD"
     gene            586..1284
                     /gene="recO"
                     /locus_tag="AC16UZ_16255"
     CDS             586..1284
                     /gene="recO"
                     /locus_tag="AC16UZ_16255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR6248292.1"
                     /translation="MSAGRRVSGEPGIVLHVRPYRETSAIVSLLTRHYGRVAGVARGV
                     RGTRRGSPVQPFSSVVTGWSGRGGLLTLTNIESVRQPWLKGEAMNAAFYVVELVTRLV
                     AEGESAPRIFAALSWALERLSSQDAELAVTLRSFEKLLLQELGYGLDFRHDASSGEPI
                     AQDVIYELDVQRGFFPGQPAEGFEGRLLLEIAEENFESAAVRQAAKRIFRLALGELLG
                     SKPLTSRRLLLRGG"
     gene            1288..2616
                     /locus_tag="AC16UZ_16260"
     CDS             1288..2616
                     /locus_tag="AC16UZ_16260"
                     /inference="COORDINATES: protein motif:HMM:NF017743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR6248293.1"
                     /translation="MSRRRIREVEVAVTALGEDGLGEGQFEGRYVRIRNALPGETVTA
                     RVLKRRAGALLAEAVEVRDRAPLRRAAPCTVFPRCGGCSLQHLEHGAQLRHKEKVLLD
                     ALAANGVVPQSVRRPVSGPRLHYRHKARLGVRCVGDNLLVGFRESFSSRVVRMADCRI
                     LAEPLARCLPDLQSTLSSLRERHRVPQVELVAGDDSQAVIVRHLDPLGPESQVRLEEM
                     SRRLRMRVYLQAGGYDSLMLLGAPGKRPKLTYSIMEFGVTYRFDVADFVQVNPAINRA
                     LASSVAVALDPRPGITIADLFCGIGNFSLVLARHGAKVKGFEASAGAIERARENADLN
                     GLEHRCEFDVADLYDSGYAIPAGTQRLLLDPPRSGAGPHLARWLSGTELERVVYVSCN
                     PMTFASDAAVFEKQGFALIDVGVFDMFPQTAHVETLGVFVPSYSRTGASR"
BASE COUNT          446 a          780 c          889 g          515 t
ORIGIN      
        1 gacgaggtcg tgcttgccca tgtccgcaag gctcgggcgg ctcgctttgc cgtaatcaac
       61 aagatcgacc tgatccgcaa caagaaccgt ctgctgcccg tcatcgagca tctgcagaac
      121 cttggctgct tcgatcaggt cataccggtt tccgcgttgc gcgatgaggg gctcgatgcg
      181 ctgcgggccg aggtggcgtc gtccttgccc cagcaccccc acatgtttcc agccgaccag
      241 ctgaccgatc agccggagcg ctttttggtg gcggagatca tccgagaaaa actcatgcgc
      301 cgctacggcg atgaaattcc tcatcgaacg gccgtcgtca ttgaggggta ccaggaggga
      361 gagcgggtta cggacatcgc cgcggacatc tatgtcgaac gcccgggaca gaaaagaata
      421 atcatcggca aaggcggcga gaagcttcgt gaggtgggaa gggacgctcg acaggacata
      481 gagaaactgc tcgatcgcaa ggtcatgctg cgattgtggg taaaagtgaa gcccggttgg
      541 acgaacgacc ggactcagct tcgacgcatg ggattcgact gagccatgtc ggccggtcgt
      601 cgcgtgtccg gtgagcccgg catcgtgctt cacgttcgcc cttacagaga gacgagcgcc
      661 atcgtctctc tgctgacgcg gcattacggt cgggtggccg gcgttgcgcg tggggtcagg
      721 ggcacgcggc gcggatcccc ggtgcaaccg ttcagcagcg tggtgaccgg ctggtccgga
      781 cgcggcggcc tgctgacatt gacgaacata gaatccgtga gacagccctg gctgaagggt
      841 gaagccatga atgcggcgtt ctacgtcgtc gagctggtga cccgtctcgt cgcggagggg
      901 gaaagcgcgc cgagaatctt cgccgcgctg agctgggcgc tggagcggct gtcatcacag
      961 gacgccgagc tcgcggtcac gctgcgcagt ttcgagaagc ttcttctcca ggagctgggc
     1021 tacgggctag attttcgcca cgacgcctct tccggcgagc ccatcgcgca ggacgtgatc
     1081 tacgagctcg acgtgcagcg cggcttcttt cccgggcagc ccgccgaggg gtttgagggc
     1141 cggcttcttc tcgagatagc cgaagagaac tttgagtcgg cggcggtcag gcaggctgcg
     1201 aagaggatat tccggctcgc gctgggggag cttctgggtt cgaaaccgct caccagtcgc
     1261 cgtctgctgc tgcggggggg gtgagcattg agccgtcgtc gcatcaggga agtggaggtg
     1321 gcggtaactg ctcttggcga ggatgggctg ggtgaagggc agttcgaggg tcggtacgtg
     1381 aggatccgca atgccctgcc tggcgagacg gtcaccgcgc gagtgctcaa gcgacgcgct
     1441 ggcgcgctgc ttgctgaggc cgtcgaggtg cgcgatcggg cgccgcttcg gcgcgcggcc
     1501 ccctgcacgg tctttccgcg ctgcggtggc tgcagtctgc aacacctcga acatggtgct
     1561 cagctgcggc acaaggagaa ggtcctgctc gatgcgctgg cagccaatgg ggtggttccc
     1621 cagagcgtga gaaggccggt ctccggtcct cggcttcact accgtcacaa agcccggctc
     1681 ggggtccgct gcgtcggcga caacctgctg gtgggatttc gcgaaagttt cagcagccgc
     1741 gtggttcgaa tggcggattg caggatcctc gccgagccgc tcgcgcgatg tctcccggac
     1801 cttcagtcaa ccctctcgag cctgcgcgaa agacaccgcg tgcctcaggt agagctggtc
     1861 gctggagatg actcgcaggc ggtgatcgtt cgtcatctgg atccgctcgg tccggaatcc
     1921 caggtcaggc tggaggagat gtcgcgaagg cttcggatgc gggtctatct gcaggccggt
     1981 ggatacgact ccctcatgtt gctcggagcg ccagggaagc ggccgaagct gacctattcc
     2041 atcatggagt tcggcgtcac ctaccgtttt gacgtggcgg atttcgtgca ggtgaaccct
     2101 gcgatcaatc gcgcgctcgc gtcgtccgta gcggtcgcct tggacccgcg gccgggaatc
     2161 accatcgccg atctcttctg cggcataggc aatttttctc tggtgctggc ccggcatggc
     2221 gcaaaggtca agggctttga ggcgtctgcc ggcgctatcg agcgggcccg ggaaaacgcg
     2281 gacctcaatg gtctcgagca ccgatgtgag tttgacgtag ccgatctgta tgattcgggc
     2341 tatgcgatcc ccgccggcac ccaacggctt cttctcgacc caccgcgttc cggtgccggt
     2401 ccccacctcg cccgttggct gagcggcacg gagctcgagc gggtggtcta cgtttcctgt
     2461 aaccccatga cgtttgccag cgatgccgcc gtcttcgaga agcaaggttt cgcgctgatc
     2521 gacgtgggcg tctttgatat gttcccgcag acggcgcacg tggaaaccct cggggttttc
     2581 gtcccctctt actcgcgcac cggggcatct cgctgatggt caaggtaggc
//
LOCUS       DBNHOX010000751         2345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1346775, whole genome shotgun sequence.
ACCESSION   DBNHOX010000751 DBNHOX010000000
VERSION     DBNHOX010000751.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1346775"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="AC16UZ_16265"
     CDS             complement(<1..464)
                     /locus_tag="AC16UZ_16265"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6248294.1"
                     /translation="MPRRPARWLLSGWCVTALLGLAACEPGHTPFPDGEIVDLSHTYD
                     ETTVFWPTADGFQLEVESAGYTDKGYYYAANNFSTSEHGGTHLDAPIHFAEGAPATDE
                     IPLERLVGPGVVIDVSAACTDDADYQIDVADVTRWERANGQTVDGMIVLFRTG"
     gene            complement(465..1130)
                     /locus_tag="AC16UZ_16270"
     CDS             complement(465..1130)
                     /locus_tag="AC16UZ_16270"
                     /inference="COORDINATES: protein motif:HMM:NF018961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HIR6248295.1"
                     /translation="MEAVEDLLAHCESLLDVTVQAVDGIAEAQGVDLETRFASDRKDL
                     YRRYLAHCLDDKILTEDENADLQHLLNLLHLNPDDVVPVHDEMAREVYGKAIQEVLAD
                     LEVDADEEAFLRRLRGDLKLSDDVASDLLERGRRDAHDVALREASTPDHDFLVYRAPA
                     GEFTGRSDVSFEAAVTDALSKAVIAIPMLHWFEVSNISGYVGDGKPRGWHVTVRGGIE
                     PEK"
     gene            1359..>2345
                     /locus_tag="AC16UZ_16275"
     CDS             1359..>2345
                     /locus_tag="AC16UZ_16275"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR6248296.1"
                     /translation="MALELPFRSAIELAHLIQTREVSSVELTRTYIERIERYDGAVNA
                     VPVRMFDQALADAAEADAVLARGEDVGPLHGVPMTIKESYVLKDTPATWGLEAFKDNV
                     ATRDGLAVSRFRQAGAHFLGKTNVPVDLADFQSYNPIYGTTGNPWNTERIPGGSSGGS
                     SAALAAGFGALEAGSDIGGSIRNPAHYCGVYGHKPTWGIVPMSGHELMEGVPDADLSV
                     CGPLARSAADLALALGIMAGPVEREAVGWRLDLPGPDIASLKSLRVAIWPNDDQAPVS
                     RETEARVVMVGETLERLGATVSDTARPDFDVRKAHINYRSLLESVMGSAKPMA"
BASE COUNT          383 a          815 c          777 g          370 t
ORIGIN      
        1 ccggtgcgga acagcacgat catgccgtcc accgtctggc cgttagcacg ctcccatcgg
       61 gtgacgtccg ccacgtcgat ctggtagtcg gcgtcgtccg tgcaggcagc gctcacgtcg
      121 atcactaccc ccggccccac cagcctctcc agcgggatct cgtcggtggc gggcgcgccc
      181 tcggcgaagt ggatgggcgc gtccaggtgc gtcccgccgt gctcggaggt ggagaagttg
      241 ttggccgcgt agtagtagcc cttatccgtg taaccggccg actccacctc cagctgaaag
      301 ccgtcggccg tgggccagaa cacggtggtc tcgtcgtagg tgtgagagag atcgacgatc
      361 tcgccgtccg gaaacggcgt atgcccaggc tcgcaggcgg ccagacccag cagggcggtg
      421 acgcaccacc cggacaggag ccatcgggcg ggacggcgcg gcatctactt ctccggctcg
      481 atcccgcccc gcacggtcac gtgccagccc ctcggtttgc cgtcgcctac atagccggag
      541 atgttggaga cctcgaacca gtgcagcatg gggattgcga tcacggcctt ggagagcgcg
      601 tcggtcacgg ccgcctcgaa agacacgtcc gaccgcccgg tgaactcgcc ggcgggggcg
      661 cggtagacga ggaagtcgtg atccggcgtg gatgcctcgc gcagcgccac gtcgtgggcg
      721 tcccggcggc cgcgctcgag caggtcggag gccacgtcgt cggagagctt cagatcgccg
      781 cgcaggcgtc gcagaaaggc ttcctcgtcc gcatccacct caagatcggc cagcacttcc
      841 tggatcgctt tgccgtagac ctcccgggcc atctcgtcgt gcacgggaac cacgtcgtcc
      901 ggattcagat gcagcagatt gagcaggtgc tgaagatcgg cgttctcgtc ctctgtgagg
      961 atcttgtcgt caagacagtg ggcaagatag cggcggtaca ggtccttgcg atcgctcgca
     1021 aagcgcgtct ccagatccac cccctgggct tcggcgatcc cgtccacggc ctgcacggtc
     1081 acgtccagaa gcgactcaca atgcgccagc aggtcctcga cggcttccat ggtactcgcg
     1141 cgcttcgagc gcagggcctg cccgaacccg gtcctcggtg gggctggtcg aaacggttct
     1201 gccacgggtc tactcctctc ctcgcgtgaa ggggggcatt gtaggccacc ttgagccgat
     1261 tgtggctacc aggccgcaag ctcctcgacg gggacgaatt gcctcccaac cggctagggg
     1321 tcattcttga gtcccgagac caacgatcag ggggaaagat ggcgctcgaa ctgcccttcc
     1381 gctcggccat cgagctggca cacctcattc agacccgcga ggtcagctcc gttgagctga
     1441 cgcgcaccta catcgagcgc atcgagcgtt acgacggcgc cgtcaacgcc gtgcccgtga
     1501 ggatgttcga ccaggccctg gccgacgccg ccgaggccga cgccgtgctg gcccgcggcg
     1561 aggacgtggg cccgctgcac ggcgtgccca tgaccatcaa ggagtcctac gtcctgaagg
     1621 acacgccggc cacctggggc ctggaggctt tcaaggacaa cgtggcgacc agagacggcc
     1681 tggccgtgag ccggttccgc caggccggcg cccacttcct gggcaagacg aacgtccccg
     1741 tggatctggc cgacttccag agctacaacc ccatctacgg caccacgggc aacccctgga
     1801 acaccgagcg catccccggc ggctcctcgg gaggcagctc ggcggccctg gcggccgggt
     1861 tcggcgccct ggaagccggc tcggacatcg gcgggtccat ccgcaacccg gcccactact
     1921 gcggcgtgta cggccacaag cccacctggg gcatcgtgcc catgtccggc cacgagctga
     1981 tggagggcgt gcccgatgcc gacctctccg tgtgcgggcc gctcgcgcgc agcgccgcgg
     2041 atctggccct ggcgctgggg atcatggcgg ggcccgtcga gcgggaggcg gtcggctgga
     2101 ggctcgacct gcccgggccc gacatcgcgt cgctcaaatc cctgcgcgtg gccatctggc
     2161 ccaacgacga ccaggctccc gtctcgagag aaaccgaggc cagagtcgtc atggtgggcg
     2221 agacgctcga gcggctcggc gccaccgtct cggacaccgc gcgtccggat ttcgatgtgc
     2281 gcaaggccca catcaactac cgcagcctgc tcgaatccgt gatggggagc gccaagccca
     2341 tggct
//
LOCUS       DBNHOX010000752         3688 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1603549, whole genome shotgun sequence.
ACCESSION   DBNHOX010000752 DBNHOX010000000
VERSION     DBNHOX010000752.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1603549"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1573
                     /locus_tag="AC16UZ_16280"
     CDS             <1..1573
                     /locus_tag="AC16UZ_16280"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6248297.1"
                     /translation="IETSLNRITRADCEQHLEPKTMAVLVFLLNRHGEVVSVDEMLDR
                     LWTGRIVGPNALHRHIALLRRALGDDPSTPSYIETITKRGYRTIASMIRLPPHGAAPS
                     DDSQHYRQEADSWGTRIAVSPFLDRTTGKSHHHLVDGMAEEVVAGLASFSDIGVIPPS
                     ALPRGDASGWDPLDTCAAVGADFFLEGSVSDSASRVRVSLRLTDVANREVIWSRSFDY
                     VLSASSYFDIQDDVKARVSAGIADSFGVVSSKAYERARTKPTSAIAADDAVMLAKGHE
                     GNPKLHREAREGLEKAVEQHPDFANAWAWLGAMYRDEFVFWANERPNPLERAESAAQR
                     ALKLDASNQEALLVLADVRFYQGDLAAFRQASERAMSLNPSSPDLLSVVASHLIHAGY
                     YEEGKPLAQRAVDLLAQPTLHMLGTLSVCHYGLGDYAAAERVIRQVWAGVDRNRISGM
                     QAYYLTLLVAATAKLGREDEAGQYLQWILKSIPNYAQIMGPSWHRQGACEDLRERLAE
                     GLAAAGLRIGELTNR"
     gene            1603..2370
                     /locus_tag="AC16UZ_16285"
     CDS             1603..2370
                     /locus_tag="AC16UZ_16285"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6248298.1"
                     /translation="MSSMRGKVALVTGASGGIGLATAIGFAERGAAVMLAARRSDELE
                     RAVREITASGGEAAAIQTDVASSEQVQEMVDRTVGTFGRLDYAVNNAGIEGVPAEFVD
                     FDEDEWDRVMNINLRGNFLCLKYQAKAMLACGHGGAIVNVGSVNSFLGFAGGAAYATS
                     KHGQVALTSSASAELAPKGIRVNIVCPGVIDTPMHRRLRGTFGDEAYDEILSRVHSRR
                     AGRPEEIASTIVFLCSDEASYLTGATITPDGGYLTTM"
     gene            2464..>3688
                     /locus_tag="AC16UZ_16290"
     CDS             2464..>3688
                     /locus_tag="AC16UZ_16290"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6248299.1"
                     /translation="MVRPEVLEHRDTISKTPILERISAMKESLAAAGDEAQELRHLPA
                     WASKEMADQGLYRFALPLELGGENLCAREQIEVVEAVSAIDGSVGWCTQISSEINALI
                     IRRMEPEFAERIFDDWDALVCSGHGPANGPNPGRLARREGDGWRLNYQGSFASGCHNA
                     NWNYLMGAMTVDEATGKPAQASFMVPLGEFEIIDTWHTAGMRGSGSHDVRMADCYVPP
                     ENLLPFRSLAPSETWQNPTYRNPTHAIYNKAAVALGVCRGAIDSFIELAMEKTPWGLS
                     STLKDQAQVQYRIGEAQATLLAARSFVMETQDKLEEHLGPLPEDGGRLEPEWEYFWPA
                     LLACAHAAQSCRKVVDMINNTAGTTGSRMDSPLERQLRDAHQAANHALISYRHYEQLG
                     RTFVGYEPPPGYVELS"
BASE COUNT          712 a         1167 c         1202 g          607 t
ORIGIN      
        1 cattgagacc agcctcaacc ggatcacacg cgcagattgc gagcagcacc tggaaccaaa
       61 gaccatggca gtgctcgtct ttctgctgaa ccgccacggc gaggtggtgt ccgtcgacga
      121 gatgctagac agactctgga ctggccggat cgtcgggccc aatgcgctcc accgccatat
      181 cgcgctgctg cgccgcgccc tgggagatga tcccagcaca ccgtcgtaca tcgagaccat
      241 taccaagcgc gggtatcgca ccattgcgtc catgatcagg cttccccctc acggggcagc
      301 gccatcagat gactcgcagc actacaggca ggaagccgac tcctggggca cccgcatcgc
      361 cgtttcgccg ttcctcgaca gaacgacagg aaagagtcac caccatctgg tcgatgggat
      421 ggcagaggaa gtcgtcgcgg ggttggccag cttcagcgat atcggcgtca tcccgcccag
      481 cgccctgccg aggggggatg catccggctg ggatcccctg gacacctgcg cggccgtcgg
      541 cgcggacttt ttcctggaag gcagcgtaag cgattcggcc tcgagagttc gtgtcagcct
      601 gcgactcacc gatgtggcaa accgagaagt gatctggtcc cgatcgttcg actacgtgct
      661 ctcggcgagc agctatttcg acatccagga cgacgtaaaa gcccgggtct ctgccggcat
      721 cgcagactct ttcggcgtgg tctcttcgaa ggcctatgag cgggcaagga cgaagccgac
      781 ttcggccatt gcagcggacg acgccgtcat gctcgccaag ggccacgagg gcaacccgaa
      841 actgcatcgt gaggcacgag agggccttga aaaagcggtc gagcagcatc ctgatttcgc
      901 caatgcctgg gcatggctcg gcgccatgta ccgggacgag ttcgtgttct gggcaaacga
      961 gcggccaaac ccactcgagc gtgcggagag cgcagcacag cgcgcgctga aactcgacgc
     1021 ctccaaccag gaagcccttc tggttctcgc ggacgtgcgg ttctaccagg gagacctggc
     1081 tgcgttccga caggcatccg agcgcgccat gagcctgaat ccgagcagcc ccgatcttct
     1141 ttccgtggtg gcgagccacc tgattcacgc cggctactat gaggaaggca aaccgctcgc
     1201 ccagcgcgcc gtggatctgc tggcacagcc aacgctgcac atgctcggca cgctgtctgt
     1261 gtgccactac ggcttgggtg attacgccgc cgccgaaagg gtgattcgcc aggtgtgggc
     1321 aggggtggac cggaaccgga tcagtggcat gcaggcctac tacctgaccc tgctggtcgc
     1381 cgccaccgca aagctcgggc gcgaggacga agccggccag tacctgcaat ggattttaaa
     1441 gagcatcccc aactatgctc agatcatggg ccccagctgg catcggcaag gggcatgcga
     1501 ggatctgcgc gagcggttgg cggaagggct cgccgcagcc ggcctccgga tcggtgagct
     1561 caccaaccgt taggctagac ggttgcataa ggacaatggg agatgagttc gatgagaggc
     1621 aaggtggcgc tcgtgacggg ggcaagcggc ggcatcgggt tggctacggc gatcggcttc
     1681 gcggagcgcg gtgcagcggt gatgctggcg gccagacggt ccgatgagct ggaacgggcg
     1741 gtgcgggaga tcacggcctc cggcggagag gcggcggcga tccagacgga tgtcgcctcg
     1801 tcggagcagg tgcaagagat ggtggatcgc accgtgggaa ccttcgggcg cctggactac
     1861 gcggtcaaca acgccggcat cgagggggtc ccggcagagt tcgtggattt cgacgaggac
     1921 gagtgggatc gcgtgatgaa catcaatctg cgcggcaact tcctctgtct gaagtaccag
     1981 gcgaaagcga tgctggcctg tggccacggc ggggccatcg tcaacgtcgg ctcggtgaac
     2041 tcgttcctgg gcttcgcggg cggcgccgcc tacgcaacct ccaagcacgg ccaggtagcg
     2101 ctcacctctt ctgcatcggc agaactcgcg ccaaagggca tcagggtgaa catcgtctgc
     2161 cccggcgtca tcgacacccc catgcaccgc cgcctgcgcg ggaccttcgg cgacgaggcc
     2221 tacgacgaga tcctcagccg cgtgcacagc cggcgggccg gccgccccga agagatcgcc
     2281 agcaccatcg tgttcctgtg ctcggacgag gccagctacc tgaccggggc caccatcacc
     2341 cccgacggtg gctatctcac gacgatgtag gcaaccggca gcccgagcaa ttacggcatg
     2401 tgcgcggcaa aatgtttatt ctttcaccct tcgggacaat ctgacttgtg aggcaaaatc
     2461 gaaatggtaa gacctgaagt tctggagcat agggacacga tctccaagac gcccattctg
     2521 gagcgcatct cggcgatgaa ggagtctctg gcggcagcgg gcgatgaagc ccaggaactt
     2581 cgtcatctgc cggcctgggc gtccaaggag atggcggatc aggggctcta tcgattcgcg
     2641 cttcctttgg aactcggcgg agagaatctc tgcgctcgcg agcagatcga ggtggtcgag
     2701 gccgtctcgg ccatcgacgg gtcggtcggt tggtgcacgc agatatcgtc agagatcaac
     2761 gcgctgatca ttcgccgcat ggagccggag tttgccgaac gaatcttcga cgactgggat
     2821 gctctggtgt gctccggtca cggccccgcg aacgggccca atcccggccg gctggcccga
     2881 agggagggcg atgggtggcg gttgaactac cagggctcat ttgcaagcgg ctgccacaac
     2941 gcgaactgga actacctgat gggcgcgatg accgtggacg aggccacagg caagccggcc
     3001 caagcctcct tcatggtgcc cctgggcgag ttcgagatca tcgatacgtg gcacaccgcc
     3061 ggcatgcgcg gctccggtag ccacgacgtg cgcatggccg actgctacgt gccgcccgag
     3121 aatctgctgc cgttccggtc tctcgcaccg agcgaaacct ggcagaaccc cacctaccgg
     3181 aacccgaccc acgccatcta caacaaggcg gccgtcgccc tcggcgtctg ccgcggcgcc
     3241 atcgactcgt tcatcgagct ggccatggag aaaacgccct ggggcttgag ctccacgctc
     3301 aaggaccagg ctcaggtcca gtaccggatc ggcgaggccc aggcgacgtt gctggccgca
     3361 cggtctttcg tcatggagac ccaggacaag ctggaggaac acctggggcc gcttcctgaa
     3421 gacggcgggc gcctggagcc ggagtgggag tacttctggc ccgccctgct cgcgtgcgcc
     3481 cacgccgcgc agtcctgccg caaagtggtg gacatgatca acaacaccgc gggcaccacg
     3541 ggcagccgga tggacagtcc cctcgagcgg cagctacggg acgcgcatca ggcggccaac
     3601 cacgcgctca tctcgtaccg gcactacgaa cagctgggca ggacgttcgt cggctacgag
     3661 ccgccaccgg gatacgtgga gctttccc
//
LOCUS       DBNHOX010000753         6284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1732723, whole genome shotgun sequence.
ACCESSION   DBNHOX010000753 DBNHOX010000000
VERSION     DBNHOX010000753.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1732723"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..147
                     /locus_tag="AC16UZ_16295"
     CDS             10..147
                     /locus_tag="AC16UZ_16295"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6248300.1"
                     /translation="MGTLEPGKLADLVVLEEDPRSVPPSRLGAVRVLETWVGGERVYQ
                     A"
     gene            190..1563
                     /locus_tag="AC16UZ_16300"
     CDS             190..1563
                     /locus_tag="AC16UZ_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HIR6248301.1"
                     /translation="MTSTFPGVIAFAFMVCMLLAGTVLRAKVPLLRRALVPASLVGGI
                     LGFALVSAGLSFGYESSDFTVFTFHFFTLSFMSLCLTGGSRERGKNTSIIPGGMWMSV
                     VWVVSLVTQGLVGLGALVGYNVLTGGELSSFLGLLATHGFTQGPGQALAYASIWESEL
                     GVVHAINFGIIYASAGFIVAFAVGVPVARHAIKAGRNVNRVARIDDEFQAGILNADSD
                     VSAGRQITHSANIDTLAFHIAVLGVAYLVTDQYLSFMRPIADGWAIGDVNFGVLFSHN
                     LFFVHGLMVCVIMRSLLDRFGLGHYIDDETQRRITGTSVDFMVVATIMSIHFGLLSEF
                     IVPILVVCLAVSAVTVVLCFGLGRHLPNLSAERSLTSFGCCSGSTGSGLLLLRIVDPD
                     LSTGIAKELAFFNIAILVLSFHVLGFMAPILPSYDLTFICAVYAATLVVGLVAVRMVR
                     KRMMAAA"
     gene            1567..2799
                     /locus_tag="AC16UZ_16305"
     CDS             1567..2799
                     /locus_tag="AC16UZ_16305"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6248302.1"
                     /translation="MDRGLRWYLLSTGLFLVPGGIQMVLFPWLVAVYLQESPARVGIA
                     QMSLQLPMLLLILWGGLVGDRFDQRRLLVGLHLGMIGPPLAMAVLVQADLIVYEVLLG
                     WAVIGGSFGAFAQPARDALLNRVAGGDVQGVVTLAIGVQFGVQIIGFALGSTADSLGP
                     LWLFLVQALLMGAAAVATSKIPLLPMGSARERRPALREIGEGIVMAWRSEAIRPAVLL
                     TFAQGVFFAGAYLVILPLMVRDLYGGGAGGIAGAFAANMLGTCTVVFFLRRRGGIDRP
                     GRALGLWGVFGVCVLSLLHFELPLWGFYAVIYLWGTCGGVSMTMSRSIVQEASPPSHR
                     ARLMSVYSLGLMGGMPFGSAFLGWCVGVLGARNAVLVPVIGMAVVQLLIFTTSRLWLV
                     ERQRGSDLDEEPLMHAAD"
     gene            complement(2817..4448)
                     /locus_tag="AC16UZ_16310"
     CDS             complement(2817..4448)
                     /locus_tag="AC16UZ_16310"
                     /inference="COORDINATES: protein motif:HMM:NF004807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HIR6248303.1"
                     /translation="MKVAQAIAKALKAEGVEVLFAYPVNPIVEAAAAEDIRTVIVRQE
                     RVGLHMADAYSRLSSGDRIGVFAMQHGPGAENAFGGVAQAYSESVPILVLPAGYHRRI
                     ANYYPNFSSTLNMRNITKWAEPLTMGKAVPEVMRRAFFQLRNGRPGPVLVEIPVDVFR
                     EDVPDDWHHTPSFKTRSAPDPDAVATAAEVLAKAERPVIYAGQGVHYAKAWAELKALA
                     ETWNIPVTTSIEGKSAFPENHPLSLGSGGRANPRPVKAFLNEADVILGIGCSFALTGF
                     GVPMPAGRTIIHATLDPMDLNKDVPAQHGLIGDAALTLRALSQAMDGKDRSPAEARAG
                     VSGRIAAIREEWLGEWMPKLTNDEAPINPYRVLWELNQMVDKDNTVITHDAGSPRDQT
                     TPFWECTTPLSYIGWGKTTQLGYGLGLAMGAKLACPEKLCINVWGDAAIGFTGMDFET
                     AVRERIPILSILFNNFSMAIEIPIMQISQAKYGATDISGNYADMAKAFGGYGERITDP
                     GDIRAALERGIEATRAGSPALLEFITDKEITVSNE"
     gene            4542..5177
                     /locus_tag="AC16UZ_16315"
     CDS             4542..5177
                     /locus_tag="AC16UZ_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HIR6248304.1"
                     /translation="MADYPSQRGIVLWAAPFLAVLLWSAVNPKDYEVWVLEVSPAVAG
                     ALIIFLTRRRFPLTRLTYGLILVHALILIVGGHYTYAEVPLFDLLREPLGFTRNNYDK
                     LGHLAQGFVPAIVAREVLLRLSPLERGGWLNLFVVSLCLGISAFYELIEWWVALLSEQ
                     AAESFLGTQGYVWDTQSDMGWALLGAVLALVLLARYHDRQLLEQGLAGNHD"
     gene            5170..>6284
                     /locus_tag="AC16UZ_16320"
     CDS             5170..>6284
                     /locus_tag="AC16UZ_16320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spinster family MFS transporter"
                     /protein_id="HIR6248305.1"
                     /translation="MTDQVEEQPAGAPAAQGFSGKTPWRSWYALGVLFVVYVFNFIDR
                     SILGILNQAIKEDLALSDTQMGFLGGIAFAVFYTFLGIPIARLADRTVRRNLLAVCLA
                     IWSVMTALCGLASSFAHLLLARIGVAVGEAGGSPPSHSMISDMFPESSRATALSIYAL
                     GIPVGTMIGNLAGGWINEAFDWRTAFIVVGLPGVALAFLVVLTVREPVRGAADNMTAS
                     SEAAPPVMTVFRYLWSLKSFRYLSLAGALHAFVGYGVGYWFPAFFIRSHGLGTGEIGT
                     WLFYLGFAGMAGTFLGGYMGDRMARRDVRWYVWLPGIATLLSVPFSMVVYLYDDYRVA
                     FLVAIVPTLLGADYLGPTFALTQGLVGLRMRALASSIL"
BASE COUNT          831 a         1994 c         2098 g         1361 t
ORIGIN      
        1 gatcacgaga tcggcaccct ggagccgggc aagctggcgg atctcgtggt gctggaagag
       61 gatccccgca gcgttccgcc gtctcgcctc ggcgccgttc gggtgctcga gacctgggtt
      121 ggcggcgaac gggtctatca ggcctgacgc gcagcctcgg gcgacgtttc acaacagaag
      181 ggtttcaaca tgacttcgac gtttccgggg gtgatcgcct ttgccttcat ggtgtgcatg
      241 ctgctggccg gaaccgtgct ccgcgcgaag gtgccgcttc tgcgcagagc gctcgtgccg
      301 gccagcctgg tagggggcat cctcggcttc gctctcgtct cggcgggact ttccttcggc
      361 tacgagagca gtgacttcac ggtgtttacc ttccattttt tcaccctcag cttcatgtcg
      421 ctctgcctga cgggcggcag cagagagcgc ggcaagaaca cctccatcat ccctggcggc
      481 atgtggatgt cggtggtctg ggtggtcagc ctggtcacgc agggtctcgt tggcctcggc
      541 gcgctggttg gctacaacgt gctgacgggc ggcgagctat cgagcttcct cggcctgctg
      601 gcaacccacg gcttcaccca ggggcctggt caggcgctcg cttacgcctc gatctgggag
      661 tcggagctgg gcgtggtcca tgccatcaac ttcggcatca tctacgcatc ggccggcttc
      721 atcgtcgctt tcgccgtggg cgtccccgtg gcgcgccacg ccatcaaggc aggacgcaac
      781 gtgaatcggg tcgctcggat agacgacgag tttcaggcag gcattctgaa cgccgactcc
      841 gatgtctccg caggccgaca gatcacccac tccgccaaca tcgataccct ggcgttccac
      901 atcgccgttc tcggcgtggc ctatctcgtg accgaccagt acttgagctt catgcggccg
      961 attgccgacg ggtgggccat cggggatgtg aacttcggtg ttctcttcag ccacaacctt
     1021 ttcttcgtcc acggcctgat ggtctgcgtg atcatgcgat cgctgctgga ccgtttcggc
     1081 ctgggccact acatcgatga cgagacccag cggcggataa ccggcacgtc ggtggacttc
     1141 atggtcgtcg ccaccatcat gagcattcat tttgggctgc tgtccgagtt catcgtgccg
     1201 atcctcgtgg tctgccttgc cgtgtctgcc gtaaccgtgg tgttgtgttt tggtcttggc
     1261 aggcacctgc ccaacctttc ggctgaacgc tcgctgacct cgttcggctg ttgctccgga
     1321 tccacgggaa gtgggctgct gctgctcaga atcgtcgatc cggacctgag cacgggcatc
     1381 gccaaggagc tcgcgttctt caacatcgcc atcttggtgc tgagctttca tgtgctcggc
     1441 ttcatggcgc cgattctgcc gagctacgat ctgaccttca tctgtgccgt ctacgcggcc
     1501 accctggtgg tgggcctcgt cgccgttcgc atggtcagga aaagaatgat ggcggctgcc
     1561 tgacccatgg atcgcggcct gcggtggtat ctgctcagca ccgggttgtt tctcgtgccc
     1621 ggtggcattc agatggtcct gttcccgtgg ctcgtggccg tgtacctgca ggaatcgccg
     1681 gcccgggtcg gcatcgccca gatgtcgctg cagctgccga tgctgttgct gattctgtgg
     1741 ggcgggctcg ttggcgaccg cttcgaccag cggcgccttc tggtcggcct gcacctgggc
     1801 atgatcgggc cccctctggc gatggcggtg ctggttcagg ccgatctcat cgtctacgag
     1861 gtgctgctcg gctgggccgt gatcggcggt tcgttcggag cgtttgcgca gcccgcgcgc
     1921 gatgctctgc tcaaccgcgt ggcgggcggc gacgttcagg gtgtggtgac gctggccatc
     1981 ggggttcagt tcggcgtgca gatcatcggg ttcgcccttg gctcgacggc cgattcgctg
     2041 ggcccgctct ggctcttcct cgtccaggcg ctgctgatgg gcgcggcggc ggtggcaacc
     2101 tcgaaaatac cgctcctgcc catggggagt gctcgggagc ggcgcccggc gctgcgcgag
     2161 atcggggagg gcatcgtcat ggcctggcgc tcggaggcga tccggccggc cgttctgctc
     2221 accttcgccc agggcgtctt cttcgcgggc gcctacctcg tgattctccc gctgatggtc
     2281 cgcgatctgt acggaggcgg cgccggaggc atcgccgggg cgtttgcggc gaacatgctc
     2341 ggcacgtgca cggtggtgtt cttcctgcga cggcgcggtg gcatcgatcg tcccggacgc
     2401 gcgctgggcc tgtggggcgt cttcggcgtc tgcgtgcttt cgttgctgca cttcgagctg
     2461 ccgctttggg gtttctatgc cgtcatctat ctgtggggca cctgcggcgg ggtcagcatg
     2521 accatgtccc ggtccatcgt tcaggaagcg tctcctccaa gtcaccgcgc ccggctcatg
     2581 tcggtctatt ccctcgggct catgggcggc atgccgttcg gatcggcttt cctcggctgg
     2641 tgcgtgggtg tgctcggcgc ccggaacgcc gtgctcgtgc ccgtcatcgg gatggccgtg
     2701 gtgcagctgc tgatcttcac cacgtcgcgg ctttggctgg tcgagcgcca acgcggcagc
     2761 gacctcgacg aagagccgct catgcacgcc gcggattgac ccgggctgcg acgcgcctac
     2821 tcgttggaga cggtgatttc cttgtctgtg atgaactcga gcagcgccgg gctgcctgct
     2881 cgggtcgcct cgatgccgcg ttccagggca gccctgatgt ccccgggatc ggtaatccgc
     2941 tcgccgtagc cgccgaaggc cttggccatg tccgcgtagt tgccgctgat gtcggtggcg
     3001 ccgtacttcg cctggctgat ctgcatgatg ggaatctcga tggccatgga gaagttgttg
     3061 aacagtatgg agagtatggg aatccgttcg cgcaccgcgg tttcgaagtc catgccggtg
     3121 aagccgatgg ccgcgtcgcc ccagacgttg atgcacagct tttcggggca cgcgagcttg
     3181 gctcccatag ccaggccaag gccgtagccg agctgggtgg tcttgcccca tccgatatag
     3241 ctcagcgggg tcgtacattc ccagaagggc gtggtctggt ctctggggct gcccgcgtcg
     3301 tgggtgatca ccgtgttgtc tttgtccacc atctggttga gctcccacag cacccggtag
     3361 gggttgatgg gggcctcgtc gttggtgagc ttcggcatcc actcgcccag ccactcctcc
     3421 cgtatggccg caatgcgccc gctcacgccg gcgcgcgctt ccgcggggct gcggtccttg
     3481 ccgtccatgg cctggctgag cgcgcgcagg gtcaacgcgg cgtcgccgat gagcccgtgc
     3541 tgcgcgggca cgtccttgtt gagatccatg gggtccaggg tggcgtgaat gatggttctg
     3601 cccgcgggca tcggcacgcc gaagcccgtg agggcgaagc tgcagccgat tcccagaatc
     3661 acgtcggctt cgttgagaaa cgctttcacg ggccgcgggt tggccctgcc gccggagccc
     3721 agcgagaggg ggtggttctc ggggaacgcg ctcttgccct cgatgctggt ggttaccgga
     3781 atgttccagg tctcggcgag cgctttgagc tccgcccagg ccttggcgta gtgaacgccc
     3841 tgacctgcgt agatgacggg cctttcggcc ttggccagca cctccgctgc cgtggcgacg
     3901 gcgtcggggt ccggcgcgct gcgggttttg aagctcggcg tgtggtgcca gtcatcgggt
     3961 acgtcctccc ggaacacgtc cacgggaatt tcgacgagca cggggccggg tcttccgttg
     4021 cgcagctgga agaaggcgcg gcgcatcacc tcggggaccg ctttgcccat ggtcaggggc
     4081 tcggcccact tggtgatgtt gcgcatgttc agcgtcgagc tgaaattggg atagtagttg
     4141 gcgatgcggc ggtgatagcc cgcgggtagc accaggatcg gcaccgattc cgagtaggcc
     4201 tgggcgacgc cgccaaaggc gttctcggcg ccgggcccgt gctgcatcgc gaacacgccg
     4261 atcctgtctc ctgacgacag gcgtgagtag gcgtccgcca tgtgcagccc cacccgctcc
     4321 tgacggacga tgaccgtgcg gatgtcctcc gccgccgccg cctcgacgat cgggttcacc
     4381 ggatatgcga acagcacctc cacgccctcg gccttgagcg ctttggcaat ggcctgagca
     4441 actttcacct ggtcacctcc catcctggct ggcgcccggc ttaccgccgg gccgtttctc
     4501 ttgtatcttg cggattctag ctcactcgta tgaggttgta gttggccgac tacccatcgc
     4561 agcggggcat cgtgctctgg gcggccccgt ttctggcggt tctgctctgg tcggctgtga
     4621 acccgaagga ttacgaggta tgggtcctgg aggtctcgcc ggcggtcgcg ggggcgctca
     4681 tcatctttct cacgcggcgg cgctttcccc tcacccggct gacgtacggg ctgattctcg
     4741 tccatgcgct gatactcatc gtgggagggc actacacgta cgccgaggtg cccctgttcg
     4801 atctcctgcg ggaaccgctg ggattcacgc gcaacaacta cgacaagctc gggcacctgg
     4861 ctcaggggtt cgtgcccgcc atcgtcgcgc gggaggttct gcttaggctt tcccccctgg
     4921 agcgtggcgg ctggctcaac cttttcgtcg tctcgctgtg ccttgggatc agcgccttct
     4981 acgagctgat cgagtggtgg gtcgcgctct tgagcgagca ggccgcagag tcgtttctcg
     5041 gcacccaggg ttacgtgtgg gatacccagt ccgacatggg gtgggcgttg ctcggcgccg
     5101 tgctggcgct cgtcctgctt gcgcgctacc acgaccggca gctgctggaa cagggtctcg
     5161 caggaaacca tgactgatca ggtggaggag cagccggcgg gggcacctgc cgcgcagggt
     5221 ttctccggca agacgccctg gcgttcctgg tatgccctgg gcgtgctgtt cgtggtctac
     5281 gtcttcaatt tcatcgaccg gtcgatcctc gggatcctca atcaggccat caaagaggat
     5341 ctggctctgt ccgacaccca gatgggcttt ctgggcggga tcgctttcgc ggtcttttac
     5401 acgtttctgg gcatacccat cgcccgcctg gccgacagga ccgtgcggcg caacctgctg
     5461 gccgtctgcc tggccatctg gagcgtgatg acggccctct gcgggctcgc ttcgagcttc
     5521 gcccacctgc tgctagcacg catcggcgtg gcggtggggg aggcgggggg cagcccgccc
     5581 tcgcactcca tgatctcaga catgtttccc gagtcgtctc gcgccacggc gctctccatc
     5641 tacgctttgg gaattcccgt aggcacgatg atcggcaacc tggccggcgg ctggatcaac
     5701 gaggctttcg actggcgaac ggccttcatc gtggtagggc tccccggggt ggcgctggct
     5761 tttctcgtgg ttctgacggt gcgcgagccg gtgcgaggcg ctgcggacaa catgacggcg
     5821 agctcggaag ccgcgccgcc ggtcatgacc gtgtttcgct acctctggtc cctcaagagc
     5881 tttcgctacc tgtcgctggc cggcgcgctc cacgccttcg tcgggtacgg cgtgggctac
     5941 tggttcccgg cgttcttcat acgcagccac ggcctcggca ccggagagat cggaacgtgg
     6001 ctgttctacc tcggctttgc gggcatggcg ggcacctttc tcggtggcta catgggcgac
     6061 cgcatggcca ggcgggacgt ccgctggtac gtgtggctgc cggggatcgc gacgctgctt
     6121 tccgtgccgt tctcgatggt cgtttacctc tacgacgact accgcgtggc gttcctggtg
     6181 gccatcgtgc cgacgttgct gggagccgac tatctcggcc ccactttcgc gctgacccag
     6241 ggactggtag ggctgcgcat gagggcgctg gcctcgagca tact
//
LOCUS       DBNHOX010000754         3753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_512873, whole genome shotgun sequence.
ACCESSION   DBNHOX010000754 DBNHOX010000000
VERSION     DBNHOX010000754.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_512873"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="AC16UZ_16325"
     CDS             <1..255
                     /locus_tag="AC16UZ_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016562481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR6248306.1"
                     /translation="IGFCQVVDPHFIRLRVYERGVGETRACGTGACAAVVAGRLLGHL
                     EDRVKVSLPGGKVRIAWQGPETTVRMAGPATLVYEGHMSL"
     gene            252..926
                     /locus_tag="AC16UZ_16330"
     CDS             252..926
                     /locus_tag="AC16UZ_16330"
                     /inference="COORDINATES: protein motif:HMM:NF016243.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HIR6248307.1"
                     /translation="MTAAQDEPTRLTDKDVISYLRLDPDFFQRHSKLLSELNLPHDTG
                     GAVSLIERQVAILRERNMAMRRRMNELVQAAKANETLFEKTRSLTLELLHVDTWHALN
                     EVLATYVLVDFHADFVCCHLRNSEIRMDHVRGHGEELPNERYSRSIDPACVTLRADEL
                     EVLFPAENHDEGGSAVLAPLVLNEAIGCLAIGSRDPAHFESDMDTLFVAYIAEVVARV
                     VQRLSP"
     gene            923..1801
                     /gene="xerC"
                     /locus_tag="AC16UZ_16335"
     CDS             923..1801
                     /gene="xerC"
                     /locus_tag="AC16UZ_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HIR6248308.1"
                     /translation="MTDPAVKRFQDYLRVEKRYSPNTLAAYRRDLERLGAVARVPWTE
                     IRGHHVSTFVAKLHAEGLAPRSIQRALSSIRSFYSYLEKIKLVASNPAAGIKAPKTRM
                     KLPKTLDTDQASQLFAFEAKTPIQKRDRAMAELLYGSGIRLSELVGIDVGDMDLSAGF
                     VRVVGKGNKVRQVPMGGHCIAAIEDWLAARGHPAGDAPLFTGRGERRISPRTVQLRLK
                     RLATQQLGSNQLHPHMLRHSFASHMLESSSDLRAVQELLGHADISTTQIYTHLDFQHL
                     AKVYDAAHPRAQSQDD"
     gene            1816..2532
                     /locus_tag="AC16UZ_16340"
     CDS             1816..2532
                     /locus_tag="AC16UZ_16340"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6248309.1"
                     /translation="MPELKVITFDLDNTLWDVDRVIINAEQRMRTWLLAHVPAFHERF
                     PPETIFELRTEIVNRQPHLKHDLSRLREEVLYQAISRCGYPEALAREYAHQAFQLFFE
                     ARHEVEFFEGALESLAILAKHYVLGALTNGNADFTKLKLDGYFSFGYSAASVGAGKPA
                     PDMFHAALEHTGAKPEQVVHVGDHPVDDIQGASQLGMHTIWVNLSGEALPPGTPKPSD
                     EVARVHDIPTAVSRIAGRGR"
     gene            complement(2545..>3753)
                     /gene="thrC"
                     /locus_tag="AC16UZ_16345"
     CDS             complement(2545..>3753)
                     /gene="thrC"
                     /locus_tag="AC16UZ_16345"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR6248310.1"
                     /translation="DLACEIIPLFVDDMSRQDLNVLIRQAYACFDHDEVVPFVRLGDL
                     EVLELFHGPTLAFKDIALQLLGRLFEHILSARGRHLNILGATSGDTGSAAIAGVRGLP
                     DIDIFVMYPHGRISPLQELQMTTVLDDNVHCLSVDGSFDDCQTLMKTVFADLAFKERY
                     ALGAVNSVNWARVLAQIVYYGYASLKRGMEFPVSFCVPTGNFGNVFAGYLAKRMGFPI
                     ERLILATNENDILAVFFETGTYQRGDVRFTVTPAMDIQVASNFERFLYYHMGEDSRPL
                     RAFMTEFAETGRAVAAIPGDDDFVATAVSVAETVEAIRATYSEFGYVADPHTAVGIAA
                     AGRFPAEGAKICLATAHPAKFPETVNGAVGAEVAVHPALEALSGLESRTTRIPATVEA
                     VKEAIQASAR"
BASE COUNT          707 a         1231 c         1193 g          622 t
ORIGIN      
        1 atcgggttct gccaggtggt ggatccgcat ttcatccggc tgcgcgtcta cgagcgaggg
       61 gtgggcgaaa cgcgggcttg cggaacgggc gcatgcgccg ccgtcgtcgc cggccggctg
      121 ctgggccacc tggaagaccg ggtgaaggta tcgttgccgg gaggcaaggt ccgcatcgcc
      181 tggcagggcc cggagaccac ggttagaatg gccggacccg ccacactggt ttacgaaggc
      241 cacatgagtc tatgaccgcg gcccaggacg agccgacgag gcttaccgac aaagacgtca
      301 tttcttacct gcgactcgac cccgacttct tccagcggca ctcgaagctg ctttccgagc
      361 tcaacctgcc ccacgacacg ggcggcgcgg tctcgctgat cgagcgtcag gtagccatcc
      421 ttcgcgaacg caacatggcg atgcggcgcc ggatgaacga gctcgttcag gcggccaagg
      481 ccaacgagac gttgttcgag aagacgcgct ccctgacgct ggagctgctc cacgtggaca
      541 cgtggcacgc tctgaacgag gtgctcgcga cctacgtgct ggtggatttc catgcggact
      601 tcgtatgctg ccatctgagg aattcggaga tccgcatgga ccacgtgcgg ggccacggcg
      661 aagaacttcc caacgagcgc tactccagaa gcatcgatcc cgcgtgcgtc accctgcgcg
      721 ccgacgagct cgaggtgctt ttccccgcgg agaatcacga tgagggcggc agcgcggtgc
      781 tggcaccgct ggtgctcaac gaggcgatcg gctgcctcgc catcggcagc cgcgatcctg
      841 ctcacttcga atcagacatg gatacgctgt tcgtcgccta catcgcggag gtggtcgcgc
      901 gggtggtcca gcgcctgtcg ccgtgaccga tcccgccgtc aagcggttcc aggactacct
      961 gcgggtcgag aagcgctact cacccaacac gctggccgcc taccggcggg atctggaacg
     1021 gctgggcgcg gtggccaggg tgccctggac cgagatccgc ggacaccacg tgagcacgtt
     1081 cgttgccaag ctgcacgccg aagggctggc tccgcgaagc atccagcgcg ccctgtcgag
     1141 catccgttcc ttctacagct atctggagaa gatcaagctg gtcgccagca accccgcagc
     1201 cggcatcaaa gcgccaaaga cccggatgaa gctgcccaag acgctcgata cggatcaggc
     1261 ctcacagctg ttcgccttcg aggccaaaac gccgattcag aaacgtgacc gcgcgatggc
     1321 cgagctgctc tacggcagcg gaattcgcct gtccgagctg gtcggcatcg acgtcggcga
     1381 catggacctg agcgccgggt tcgtgagggt cgtcggcaaa ggcaacaaag tcaggcaggt
     1441 ccccatgggc gggcactgca tcgcggccat cgaagactgg ctggcagccc ggggacatcc
     1501 cgccggcgac gcgccgctgt tcaccggtcg gggcgagcgg cgtatcagcc cccgaaccgt
     1561 ccagctgcgc ctaaagcgcc tggccaccca gcagctcggc agcaaccagc tgcacccgca
     1621 catgctcagg cacagctttg ccagtcacat gctcgagtcg tccagcgacc tgcgggcggt
     1681 tcaggaactt ctcggccacg ccgacatctc aacaacccag atctacacac atctggactt
     1741 ccagcatctg gcaaaggtgt acgacgccgc ccatccccgc gcgcaatccc aggacgactg
     1801 attaggcccc tgatcgtgcc cgagctgaaa gtcatcacct tcgatctcga caacaccctg
     1861 tgggatgtgg atcgggtcat catcaacgcc gagcagcgca tgcggacgtg gctgctcgca
     1921 cacgttccag ccttccacga acgttttccg ccggagacga tcttcgagct gcgcacggaa
     1981 atagtgaaca ggcagcccca tctgaaacac gacctgtccc gactgcgcga agaggttctc
     2041 taccaggcca tcagccgctg cggctacccg gaagcgctgg cccgagaata cgcccatcag
     2101 gcctttcagc tgttcttcga ggcgcgccac gaggtggagt tcttcgaggg tgcgctggaa
     2161 agcctggcca tcctggccaa gcactacgtc ctcggcgctc tgaccaacgg caacgccgac
     2221 ttcaccaagc tcaagctgga cggctatttt tctttcggtt actccgcggc cagcgtcggt
     2281 gccggcaagc ccgcgccgga catgtttcat gcggccctcg agcacacggg agcgaagccc
     2341 gagcaggtgg tccacgtggg cgaccacccg gtcgacgata tccagggcgc atcccagctc
     2401 ggcatgcaca ccatctgggt caacctgagc ggcgaggcgc tgccgcccgg gaccccgaag
     2461 ccctcggacg aggtcgcaag ggtgcacgac attcccacgg ccgtatcccg catcgccggg
     2521 cgcggccgct gatccgaatc gccctcagcg ggcgctggcc tgaatcgcct ccttgaccgc
     2581 ctcgacggtg gccggaatcc gggtcgtacg cgactcgagt cccgacaggg cctccagggc
     2641 cgggtgcacg gcgacctcgg cacccaccgc cccattgacc gtttccggga acttggccgg
     2701 gtgcgcggtg gcaaggcaga tcttcgcgcc ttcggccggg aagcggccgg cagcagcaat
     2761 gccgacggcc gtgtgcggat cggctacgta gccgaactcc gagtaggtgg cgcggatcgc
     2821 ttcgacggtc tcggccacag agactgcggt ggccacgaag tcgtcgtcgc cgggtatcgc
     2881 ggcaaccgca cgaccggttt cggcgaactc ggtcatgaac gccctcagcg gtcggctgtc
     2941 ctcgcccatg tggtagtaaa ggaaccgctc gaaattgctg gccacctgaa tgtccatggc
     3001 gggtgtcacg gtaaatcgca cgtcgcctcg ctggtaggtg cccgtttcga agaacacggc
     3061 gaggatgtcg ttctcgttgg ttgccagaat cagccgttcg atgggaaatc ccatgcgctt
     3121 ggccaggtag cccgcgaaga cgttgccgaa gtttccggtg ggcacgcaga aggagacggg
     3181 aaactccatg ccccgcttca ggctcgcgta gccgtagtag acgatctgcg ccagcactct
     3241 cgcccagttc acggagttga cggcgcccag cgcgtaacgt tccttgaatg ccagatccgc
     3301 gaagacggtt ttcatgagcg tctggcagtc gtcgaagctg ccgtccaccg aaaggcaatg
     3361 gacgttgtcg tccagcaccg tggtcatctg cagctcctgc agcgggctga tacgcccgtg
     3421 gggatacatg acgaagatgt ctatgtcggg cagcccgcga acgccggcga tggccgcgct
     3481 gccggtatcg ccgctggtcg caccgagaat gttcagatgc cggccgcggg ccgagaggat
     3541 gtgctcgaag agacggccga gaagctgcag agcgatgtcc ttgaaggcca gggtaggccc
     3601 gtgaaagagc tccagcacct ccagatctcc cagccggacg aacggaacca cctcgtcgtg
     3661 gtcgaagcag gcgtatgcct gacggatcag gacgttcaga tcttggcgcg acatgtcgtc
     3721 gacgaaaagc gggatgatct cgcaggcgag gtc
//
LOCUS       DBNHOX010000755         4479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1282177, whole genome shotgun sequence.
ACCESSION   DBNHOX010000755 DBNHOX010000000
VERSION     DBNHOX010000755.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1282177"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="AC16UZ_16350"
     CDS             <1..399
                     /locus_tag="AC16UZ_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR6248311.1"
                     /translation="ALAICYEIVYPNLVRAQAVEADVLVTISNDTWFGASIGPLQHLQ
                     MARMRALENGRWLLRGTNNGVTAIVDHRGRLVARLPQFEAGVLRGEYRLVAGRTPYNR
                     HGDGPVLVLVAFGLGVAFWRRLGAARGADG"
     gene            415..945
                     /locus_tag="AC16UZ_16355"
     CDS             415..945
                     /locus_tag="AC16UZ_16355"
                     /inference="COORDINATES: protein motif:HMM:NF016290.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly lipoprotein LptE"
                     /protein_id="HIR6248312.1"
                     /translation="MRTRLRYTWLIPPLFLLIGGCGFQLRTWDLGTSVESFYVRDASN
                     NPLASPLRRALRQAGVDEAGNASDAQVVVLLLNAQRGRRSVSVTGQARAAEYEISLGV
                     QYGVLDGQGQELIAPRWVRGTRVFRVDRDNIVGTSEEQALLEREIQNDLIQQIVRALN
                     TVSGPAGPAGQTANAA"
     gene            935..1939
                     /gene="holA"
                     /locus_tag="AC16UZ_16360"
     CDS             935..1939
                     /gene="holA"
                     /locus_tag="AC16UZ_16360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR6248313.1"
                     /translation="MPLRLSDLQSRLAAGLAPVYFISGDETLLVQEACDAILGKAHSE
                     GFTERSVFHVEKGFRWNDILQDAASLSLFAERRIIDVRVPGNTFDREASEVLRTYSAE
                     PSPDTLLLIRSARLEPRQRSSAWFKALDAAGAVVLVWPVGIAELPRWLAGRLRAAGLS
                     LTPDAQSYLAQQVEGNLLAAVQEVEKLRLADLPQPIGSEELMRQMEDSAHYDSFELID
                     AVFAGDGARVSRILANLRQEGVSLYAVLAALTGQLRRLPGGRMAPQRQRLVDGFMRRM
                     RSPRNLDRALAQCALVDAQGKGQLLGDAWLSLENVCLRLAGQRGLPSLEEELPSLRRA
                     "
     gene            complement(1978..3171)
                     /locus_tag="AC16UZ_16365"
     CDS             complement(1978..3171)
                     /locus_tag="AC16UZ_16365"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6248314.1"
                     /translation="MAELEVLTHDEEMMSLARRALEHFVNKTTDQAASTLEMPVEAYV
                     GQSRYQAERDRIFMHLPLALALSLELPEPGCYRAMTVMNVPVLLIRGEDDTVRAFVNV
                     CTHRGARLCPEGAGERRVISCPYHAWTFDFEGRLSGRYAAETFGDLDPEAYALTELAC
                     AERAGVIWVILTPGEQFDADAWLGGFAEQLGSLHLTDWYLYEQRDLEGPGWKVAMDGY
                     LEIYHHNPLHGATVGQHTVGNLLVLDTYGPHQRLTLGRRTLGTLAETPESEWAPLEHI
                     RLIHSGFPNLSVSGILGDHCLVSQIFPGEDPFGTVTRQSILVAEKPATDAAEAAARNF
                     SDMVLQAVRDEDYAIGQTIQAGLRAPARAAFIFGRNEPAVQNYHRWVANFMTQTAEAR
                     DWGGV"
     gene            complement(3158..3721)
                     /locus_tag="AC16UZ_16370"
     CDS             complement(3158..3721)
                     /locus_tag="AC16UZ_16370"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6248315.1"
                     /translation="MSELDELRTRVDRLESREAIRELVSAYAVACDHHDMPALDDLFT
                     EDAVFASPNGAMIATGRNAIHDMFVRILGIRGPGYHWTHDVTVNIDPSDPDRATGVVH
                     SHAETTPSGVMSIAAMRYHDSYRREAGIWRFASREISFLYYVPVSEYGASLTRPDRVV
                     MGTERHAADYPEALEAWQAFEASHGGA"
     gene            4030..>4479
                     /locus_tag="AC16UZ_16375"
     CDS             4030..>4479
                     /locus_tag="AC16UZ_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6248316.1"
                     /translation="MTVNQSGLSADMAPDTTVSAREQFGVEHDFQVPAFSKRSDYVPE
                     VDVDYRFDPDTTLAILAGFAHNRRVMIAGYHGTGKSTHIEQVAARLNWPCIRVNLDSH
                     ISRIDLIGKDAIVLEEGKQVTEFREGILPWAIQHPVALVFDEYDAGRP"
BASE COUNT          717 a         1456 c         1528 g          778 t
ORIGIN      
        1 gcgctggcca tctgctacga gatcgtctac ccgaacctcg tcagggctca ggccgtcgag
       61 gcagacgtgc tcgtgaccat atccaacgac acctggttcg gtgccagcat cggaccgctt
      121 cagcatctgc agatggcaag gatgcgcgct ctggaaaacg gccgctggct gcttcgggga
      181 accaacaacg gggtgaccgc catcgtcgac caccggggca gattggttgc gcggctgcct
      241 cagttcgagg ccggcgtgct gcggggcgag taccgactcg tggcaggccg aacgccctac
      301 aaccgtcatg gtgatgggcc ggttctggtg ctggttgcct tcggtctcgg cgtcgccttc
      361 tggcgcaggc tcggcgcggc gcgtggcgcc gacgggtagt gtagtatgcg gcgcgtgcgc
      421 acccgtctac gctacacctg gctgatcccg cctctgttcc tcctcatcgg gggctgcggc
      481 tttcagctgc gcacatggga tctcggcacc agcgtggaga gcttctacgt cagggacgcc
      541 agcaacaatc cgctggcatc gccgcttcgg cgggcccttc gtcaggccgg cgtcgacgag
      601 gcgggcaacg cttcggatgc ccaggtggtg gtgctgctgc tgaacgccca gcggggacgg
      661 cgcagcgtgt cggtcaccgg tcaggctcgg gcggccgagt acgaaatttc gctcggcgtt
      721 cagtacgggg tgctcgacgg gcagggacag gagctcatcg cgccgcgctg ggtccgcggg
      781 acccgcgtgt tccgggtaga ccgggacaac atcgtcggca ccagcgagga gcaggcgctg
      841 ctcgagcggg aaatccagaa cgatctcatc cagcagatcg tcagggcgct caacacggtt
      901 tccggcccgg ccggcccggc gggtcagacg gccaatgccg cttaggctgt cggacctgca
      961 aagccgtttg gccgccggcc ttgcgcccgt gtacttcatc tccggtgacg agaccctgct
     1021 ggttcaggaa gcctgtgacg cgatcctggg caaggcgcac tcggaggggt tcacggaacg
     1081 gtccgtgttc catgtcgaga agggattccg ctggaacgac attcttcagg acgctgcgag
     1141 cctgtcgctg tttgccgagc ggcggatcat cgacgtccgg gtgcccggca acacgttcga
     1201 ccgggaagcc tcggaggttc tgcgtaccta ctcggcagag ccctcgccgg acacccttct
     1261 gctcatccgc tcggcgcggc tcgagccgcg acaacgatcg tcggcctggt tcaaagccct
     1321 ggatgcggcc ggcgcggtgg tcctcgtgtg gccggtgggc attgccgagc tgccgcgctg
     1381 gctggccggg cggctgcgcg cagccgggct gtcgctgacg ccggacgccc agagctacct
     1441 ggcgcagcag gtggagggca atctgctcgc tgcggttcag gaggtggaga agctcaggct
     1501 tgccgatctg ccccagccca tcggcagcga ggaactgatg aggcagatgg aagactctgc
     1561 ccactacgac tccttcgagc tcatcgatgc ggttttcgcg ggcgatgggg caagggtgag
     1621 ccgcatactg gccaatctcc ggcaggaggg cgtttccctc tacgccgttc tggctgcact
     1681 gactggtcag ctccggcgtc tgcccggcgg gcggatggcg ccccagcgcc agcgcctggt
     1741 cgacggcttc atgcgtcgca tgaggtcccc gcgcaacctc gacagggccc tggcccagtg
     1801 cgcgctcgtg gacgctcagg gtaagggcca gctgctcggg gatgcctggc tttctctgga
     1861 gaacgtctgt ctgcggctgg cgggacagcg cggcctgccg tctctcgagg aggagctgcc
     1921 ttcgctgcgc agagcctgat cgtgcccggc gccaagcggg cgatcagcag gcccggctca
     1981 caccccgccc cagtcccgcg cctcggcggt ctgggtcatg aagttggcca cccagcggtg
     2041 gtagttctgc accgcgggct cgttgcggcc gaagatgaag gcggcgcgcg caggggcacg
     2101 aagcccggcc tgaatggtct ggccgatggc gtagtcctcg tcgcggaccg cctggagcac
     2161 catgtcggag aaattgcggg cagcggcctc ggcggcgtcc gttgccggtt tctccgccac
     2221 caggatgctc tgccgggtga cggtgccgaa cgggtcctct cccgggaaga tctggctgac
     2281 caggcagtgg tcgccgagga ttcctgagac agacaggttg ggaaacccgg agtggatcag
     2341 gcgaatgtgc tcgagcggag cccactcgga ctccggcgtt tccgccagcg tccccagcgt
     2401 gcggcgcccc agggtgagcc gctggtgggg gccgtaggta tccaacacca gcaggtttcc
     2461 cacggtgtgt tgccccacgg tggcaccgtg cagcgggttg tgatgataga tctccaggta
     2521 gccgtccatg gcgactttcc agcccgggcc ttcgagatcc cgctgctcgt acagatacca
     2581 atccgtgaga tgcagcgaac ccagctgctc ggcaaacccg ccgagccagg cgtccgcgtc
     2641 gaactgctcg cccggggtga gtatcaccca gatgacgccg gcccgctcgg cgcaggcaag
     2701 ctccgtgagc gcgtaagcct cggggtccag gtcgccgaag gtctccgccg cgtaccggcc
     2761 cgacagtcgg ccctcgaagt cgaaagtcca ggcgtggtag ggacagctga tgaccctgcg
     2821 ctctccggca ccttccgggc acagccgtgc cccccggtga gtgcagacgt tgacaaaagc
     2881 acgaacggtg tcgtcttccc cgcggatgag caggacgggt acgttcatga ccgtcatggc
     2941 ccggtagcag ccgggctcgg gcagctcgag gctcaatgcc agcgccagcg ggagatgcat
     3001 gaagatgcgg tcccgctctg cctggtagcg cgactgcccc acataggcct ccaccggcat
     3061 ctcgagggtg gatgccgcct ggtcggtcgt cttgttcacg aagtgctcga gcgcgcggcg
     3121 cgccagcgac atcatctctt catcgtgggt caagacttca agctccgcca tgactcgcct
     3181 cgaacgcctg ccatgcctcc agcgcctcgg ggtagtcggc ggcgtgtcgc tcggtgccca
     3241 tgaccacgcg gtcggggcgc gtcaggctgg ccccgtactc ggaaacgggc acgtagtaca
     3301 gaaagctgat ctcgcggctg gcgaaacgcc agatgccggc ttctctgcga tagctgtcgt
     3361 gatagcgcat ggccgcaatg ctcatcacgc cgctgggcgt ggtctccgcg tggctgtgca
     3421 ccacgccggt ggcgcggtcg gggtcgctcg gatcgatgtt gacggtcacg tcgtgggtcc
     3481 agtggtaacc cgggccccgg ataccgagaa tccgaacgaa catgtcgtgg atggcgttcc
     3541 ggcccgtggc gatcatggcg ccgttcggtg aggcgaacac ggcatcctcg gtgaacagat
     3601 cgtccagcgc cggcatgtcg tggtggtcgc aggcaacggc gtaggccgac accagctcgc
     3661 ggatggcttc tctggactcc agacggtcca ctcgtgtgcg caattcgtcc agctcgctca
     3721 agatcgcctc cccagctctt catcgcttga ttcaactgaa tatagagggc gctcaccacg
     3781 cgatcaacaa ggcccgcagc cggcccgcgc ccaatcggct ggagagggcc tgcagacggc
     3841 ggaaatcggg cgttcgagcg ggcttgagcc ggtctgccgc ccgggcgtcc tcgcggattt
     3901 gaccaactct ttgcccaggt ggtgcgaaaa cgccggcctg gcgggcagcg agattaatcg
     3961 cccgcatctg ctgctctttt accggtggtt ccttacaata aacggctcgc ccgcagttga
     4021 ggaattgcag tgacggtaaa ccaatccggc ctgtccgcgg acatggctcc ggacacgacg
     4081 gttagtgccc gggaacagtt cggcgtcgag cacgacttcc aggtgccggc gttcagcaag
     4141 cgatcggact acgtgccgga ggtggacgtg gattaccgtt tcgatccgga caccacgctt
     4201 gcgatcctcg ctggtttcgc ccacaaccgc cgggtgatga ttgccggcta ccacggcacg
     4261 ggtaagtcca cccacatcga gcaggtggcc gcgcggctga actggccgtg catccgcgtg
     4321 aacctcgaca gccacatcag ccggatcgac ctcatcggca aggacgccat cgtgctggag
     4381 gagggcaagc aggtcaccga gttccgcgaa ggcatcctgc cctgggcgat ccagcatccg
     4441 gtggcgctgg tattcgacga gtacgacgcg ggccgcccc
//
LOCUS       DBNHOX010000756         2249 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1668600, whole genome shotgun sequence.
ACCESSION   DBNHOX010000756 DBNHOX010000000
VERSION     DBNHOX010000756.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1668600"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="AC16UZ_16380"
     CDS             complement(<1..540)
                     /locus_tag="AC16UZ_16380"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6248317.1"
                     /translation="MADPNTASDHDRYNSAAMGYAVKLHEARAEARKRAKVAQRSPEQ
                     LFPRSNRPGLGVALEYERMGNRPVSEDLLARAGEHGVEFEHLGITLGTVLHNISLQDP
                     LSPAQVQLVRDTLLERKVIFFRDQHLSEAEQVAFSRQFGELDAFPFGKPGRNPYILEI
                     VHDENSPGTENGFHTDVTWM"
     gene            complement(645..>2249)
                     /locus_tag="AC16UZ_16385"
     CDS             complement(645..>2249)
                     /locus_tag="AC16UZ_16385"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6248318.1"
                     /translation="ALRRSAVPGPADEGRRRIAVVTAKDRAGAPDGNHVDVLIVGAGI
                     SGVGSAYHLQQQCPEKSYVILEMKDTFGGTWETHKYPGIRSDSDLYTFGYRFKPWIGA
                     PIASAAEILKYMGEVIEENGIEQHIRYGHRINRCSWSSADNLWTVEATRLSDGAVFTF
                     TCNFLWMCQGYYDHENPFTPDWPGMDRYQGQFIHAQLWDPEIDYTGKRILVIGSGATA
                     ATVVPAFAEKAEHVTMLQRSPTYFYCRENRNELADRLREIGIDEPTVHRVVRAQIIHD
                     QNEMTKRCLAEPDAVFEDLKALIRLYAGEDFEFEPHFVPKYRVWQQRLAFCPDGDVFR
                     AAAAGKLTVVTDTIDTFTEKGVRTASGEEIEADIIFAATGFRLSVMGDIPFDVDGRPV
                     DWHETVNYRGMMFTGVPNLLWVFGYFRASWTLRVDMLGDFVCRLLRHMDAIGAKRVDV
                     ELREEDADMELLPWIEEENFNPGYLMRGLDELPRRGAKPEWRHNQDYWTEREQIPAID
                     LDGEEFVYDGARRSGATVRGKAASAS"
BASE COUNT          396 a          742 c          664 g          447 t
ORIGIN      
        1 catccaggtg acgtcagtgt ggaacccgtt ctccgttccg gggctgttct cgtcatggac
       61 gatctccagg atgtagggat tcctgccagg cttgccgaac ggaaaggcat ccagctctcc
      121 gaactgcctg ctgaacgcca cctgctctgc ttccgagaga tgctgatcgc gaaaaaagat
      181 cacctttcgc tccagcaacg tgtcgcggac cagctgcacc tgagctggcg atagcgggtc
      241 ttgcagactg atgttgtgca gcacggtccc gagggtgatg cccaggtgct cgaactcgac
      301 gccgtgttct ccggcccgcg cgagaaggtc ttccgagacc ggcctgtttc ccatgcgctc
      361 gtactccaga gcaacgccca gccccggccg gttcgaccgt gggaagagct gctccgggga
      421 gcgctgcgcc accttggcgc gctttcgagc ctcggccctg gcttcgtgaa gctttaccgc
      481 ataacccatc gcggcactgt tgtagcgatc gtggtcgctc gcggtgttgg gatctgccat
      541 gttgctcgcc tcttctgcag aggggagcat acgtcaatcg ccttctccac agccaccggg
      601 cggcgggatc aggccctcgc gcaggctgct catgtcctct gctctcagga agcggaggcc
      661 gccttgccgc ggaccgtcgc cccgctccga cgcgccccgt cgtagacgaa ctcctcacca
      721 tcgaggtcga tggccgggat ctgttcgcgc tcggtccagt agtcctgatt gtgccgccat
      781 tcgggcttgg cgccgcgccg cgggagctca tccagacccc gcatgaggta gcccggattg
      841 aagttctcct cctcgatcca gggcagaagc tccatatccg catcctcttc acgcaactcg
      901 acgtccaccc gcttagcgcc tatggcatcc atatgcctca agagcctgca gacgaaatca
      961 ccgagcatgt ccacgcgcag ggtccagctg gcacggaagt atccgaacac ccacaacagg
     1021 ttgggcacgc ccgtgaacat catcccgcgg tagttgaccg tctcgtgcca gtcgacgggc
     1081 cgcccatcga cgtcgaaagg tatgtccccc ataaccgaca ggcgaaaacc cgtcgcggca
     1141 aagatgatgt ccgcttcgat ctcctcaccc gaggcggtgc gaacgccttt ctcggtaaag
     1201 gtgtcgatgg tgtcggtcac gacggtgagc ttgccggcag cggccgcccg gaacacgtcg
     1261 ccatccgggc agaaggcaag ccgctgctgc cacacccggt actttggaac gaagtgcggc
     1321 tcgaactcga agtcctctcc ggcatacagt cgaatcagag ctttgagatc ttcgaacacc
     1381 gcatccggct cggccaggca gcgcttcgtc atctcgttct gatcgtgaat gatctgagca
     1441 cgaaccacgc gatgcaccgt tggctcgtcg atgccgatct cccgcaggcg gtcagcgagc
     1501 tcgttcctgt tctcccggca gtagaagtac gtcggcgatc gctgcagcat ggtcacgtgc
     1561 tcggccttct cggcaaaggc cgggaccacg gtcgccgccg tggcacccga cccgatcacc
     1621 agaatccgct tgcccgtgta atcgatctcg ggatcccaga gctgggcgtg gatgaactgc
     1681 ccctggtagc ggtccatgcc aggccagtcg ggcgtgaaag gattttcgtg atcgtagtag
     1741 ccctgacaca tccacaggaa gttgcaggtg aacgtgaaga cggcgccgtc cgaaagccgg
     1801 gttgcctcca ccgtccatag gttgtccgcg ctcgaccagc tgcagcggtt gattcgatgg
     1861 ccgtaacgga tgtgttgctc gatcccgttt tcctcgatga cctcgcccat gtacttcagg
     1921 atctcggcgg cgctcgcgat aggcgcgccg atccagggct tgaagcgata tccgaacgtg
     1981 tagaggtccg agtcggagcg gattcccgga tacttgtggg tttcccaggt tcctccgaag
     2041 gtgtccttca tctcgagaat cacatagctt ttctcggggc actgctgctg caggtggtac
     2101 gccgagccga ctccggatat gcccgcccca acgatcagga cgtccacatg gtttccgtcc
     2161 ggcgcgcccg cccgatcttt cgcggtgacc acggcaatcc tccttcttcc ttcgtcggcc
     2221 ggcccgggga cagcagaccg cctgagagc
//
LOCUS       DBNHOX010000757         1992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1604198, whole genome shotgun sequence.
ACCESSION   DBNHOX010000757 DBNHOX010000000
VERSION     DBNHOX010000757.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1604198"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..648
                     /locus_tag="AC16UZ_16390"
     CDS             322..648
                     /locus_tag="AC16UZ_16390"
                     /inference="COORDINATES: protein motif:HMM:NF018500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HIR6248319.1"
                     /translation="MPARSRLLDCLHRHDCVVARVRHVTDPLDPRERPPPVPIPHTEV
                     SAEALRGIIESFVLREGTDYGARDVPLESKVTQVLNQLERGEAQILFDPLTESIDIVA
                     VARRNR"
     gene            645..998
                     /locus_tag="AC16UZ_16395"
     CDS             645..998
                     /locus_tag="AC16UZ_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248320.1"
                     /translation="MRGRLAGSSASRKDGRSPGTDGGVHLAPGFCAFLDRGIQDSEPN
                     SIEFRPSVASGPPSDPLAHSDNALFAADHTASLISLVSTLRSTGYFKVRVRRVGDSAG
                     RKYFQGSLSLAEFWY"
     gene            1180..1608
                     /locus_tag="AC16UZ_16400"
     CDS             1180..1608
                     /locus_tag="AC16UZ_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248321.1"
                     /translation="MVLALGVSSMMIATGAVAQEWPFVPGDYWEITGIDIKDGGGLKY
                     SQWLASEWRENLEFSKSKGWIKGYKVFSNVYGRANEPDIYLITITDSIVSGAESEKRS
                     QEYRAWRKKTIEQMQSESGNRAEYRTVMSDSLIQELNFRK"
     gene            complement(1691..>1992)
                     /locus_tag="AC16UZ_16405"
     CDS             complement(1691..>1992)
                     /locus_tag="AC16UZ_16405"
                     /inference="COORDINATES: protein motif:HMM:NF039822.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HIR6248322.1"
                     /translation="LEVRGFEEASARTMTAVLLEREDLDARRELQGTATNVAPPSLQV
                     LGVSVTTSGTTEFRDASDQIMDPTLFFQQAPNRLVKVRGTWNGSAFSATHAELES"
BASE COUNT          336 a          647 c          661 g          348 t
ORIGIN      
        1 caggctccac cggtacaggt acagcttcca cagcccggtc tcggtccggt gcagatgctc
       61 gcagcacggc ctggggaagc tcccgacgtg gacgacgttc ccctcggtgc ggaaccggag
      121 ttccttgacg cacctcaact ctcgctcagt actctgctgg atggacgtca tcgggatgcc
      181 acggcgagtt tcaggctgct gcgcggatcc gtccggtacc cacgctgtgg tccatggcgc
      241 ttcctccctt ccgcggagcc gggcggcctc attcccctgc ctcgattatc gggggagggt
      301 gggtcaggcg gtgacccagg cgtgcccgca cgatcccggt tgctagactg cctgcatcgc
      361 catgactgcg tcgttgccag ggtgcgccac gtgaccgacc ccctcgatcc ccgcgagcga
      421 ccgccgccgg tgccgattcc gcacaccgag gtctcggccg aggcgctgcg cgggatcatc
      481 gagtccttcg tgctgcgcga gggcaccgac tacggcgcgc gggatgtgcc cctggagagc
      541 aaggtgacgc aggtgctcaa ccagctggag cggggcgagg cgcagatcct gttcgatccg
      601 ctgaccgaga gcatcgacat cgtcgccgtg gcccgccgga accggtgaga ggtagactcg
      661 ccggatcctc ggcgtcccgg aaggatggcc gaagtccagg cacagacggt ggtgttcacc
      721 ttgccccggg tttctgcgcg ttcctggatc gcgggatcca ggacagcgag ccgaactcca
      781 tcgagttccg acccagcgtc gccagcggtc ccccgagcga cccgctcgct cactccgaca
      841 atgcgctgtt cgcagccgac cataccgcga gtctcatatc gctcgtgtcg acgctcaggt
      901 ccaccgggta tttcaaggtc cgggtcagac gggtggggga ttcggccggt cgcaagtact
      961 tccagggctc gctgtcgctg gcggaattct ggtactgagg gctcgacacc ctgcaaaccg
     1021 gcgccccctg cggttgcgcg actccggcct ggatccggag gccccgcccg cctcatgtca
     1081 tgccgaggaa gtcaccgtgt tagactaagt ccagaaaacg ggcaagcctt gtccgttgtg
     1141 aggaggggga tagtgagaat gtcaatcagg aagtcggtca tggtgctcgc actcggcgtt
     1201 tcatccatga tgatcgccac gggcgccgtg gcccaggagt ggccgttcgt gccgggcgat
     1261 tactgggaaa tcaccgggat cgacatcaag gatggcggtg gactgaagta ctcccagtgg
     1321 ctggcgagcg aatggcgcga gaacctggag ttctcgaagt ccaagggctg gatcaagggc
     1381 tacaaggtct tctccaacgt ctacggccgc gcgaacgagc cggacatcta cctgatcacg
     1441 atcacggaca gcatcgtctc gggtgccgag agcgagaagc gctcgcagga ataccgggcg
     1501 tggcgcaaga agaccatcga gcagatgcag agcgagagcg gcaaccgggc cgagtaccgg
     1561 accgtgatga gcgactcgct gatccaggaa ctcaacttcc gcaagtgatg gctcgattcc
     1621 ggtaatcgac caacagaaac cccggcctcg cgccggggtt tctttttgtc cgccagccgc
     1681 ccggacccgg tcagctctcg agctcggcgt gggtggcgct gaacgcgctg ccgttccagg
     1741 tgccgcgcac cttgacgagg cggttcggcg cctgctggaa gaacagcgtc gggtccatga
     1801 tctggtcact ggcgtcgcgg aactccgtgg tgccgctggt cgtgacgctg accccgagca
     1861 cctgcaggct gggcggcgcg acattggtcg ccgtgccctg cagttcgcgg cgcgcgtcga
     1921 ggtcctcacg ttccagcagc acggcggtca tcgtgcgcgc cgacgcttcc tcgaagccgc
     1981 gcacttccag gt
//
LOCUS       DBNHOX010000758        13858 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1668795, whole genome shotgun sequence.
ACCESSION   DBNHOX010000758 DBNHOX010000000
VERSION     DBNHOX010000758.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13858)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13858)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13858
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1668795"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="AC16UZ_16410"
     CDS             <1..912
                     /locus_tag="AC16UZ_16410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6248323.1"
                     /translation="TPLAYIAAHTERIRLGTGVIQLAARTPANAAMAFATLDQLAGGN
                     RAICGIGVSGPQIVEGWYGQPWGRPYYRIKDYVAIMKKIFAREEPVTHAGKEISLPYQ
                     GEGALGIGKPLKSILHMNPNIPIFLGTGMESTVRLTAEVADGWLPLGFVPETAALYDG
                     WIDEGLAKGGKTSEQFERQAGTSVQVTDDVKSALDRLKPGIALYVGGMGHKTKNFHKE
                     MMIRRGFGEAAERIQELYLAKRKDEAIAAVPDEFVDQGALVGPKERIARRFRDWEDSG
                     LTGLTISGDEEAVRFMAECARLNFAAE"
     gene            complement(924..2327)
                     /locus_tag="AC16UZ_16415"
     CDS             complement(924..2327)
                     /locus_tag="AC16UZ_16415"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR6248324.1"
                     /translation="MQSLARNPDDPVSATIRSPVDPGVSRHWCQGALASMLGCALLWS
                     SCAAADQAGGWYAGVGIGWNHAENMELDELGGVVDYDFGAPVWSAALGWAFGPDWRVE
                     VEASRQRNDVELVSFSAPVSASAAALDPAPENAFSATTLAINAVREFAVGRDFRPYLG
                     AGVGLAWTELDITDGSIEAQIDPEPRDPLVDDQATSFAFQLIGGISLPLTRRMDLGVE
                     YRYWQAPSVKLKEFTGTKLDTKHAVHSGWLQLRYRSGGVERSRPAASPRPSPRGFYLT
                     TFAGAGFSVDVEIDPGQTNFDALDVGPVAAFGVGYALSRRWRFELETAFRKNEVELLD
                     FGLRFGSKQGSGRIRSTSLLASGIYQFFPEARTRPYLGVGAGLVWADYDVDVLDELFV
                     DDKDQSYAAEFRLGAEVSVSQRLTVSAEYRYWISSDLKMTGPDGGPVEAYQQTHSMMV
                     GLRYALGSGRSAGASGR"
     gene            complement(2299..3138)
                     /locus_tag="AC16UZ_16420"
     CDS             complement(2299..3138)
                     /locus_tag="AC16UZ_16420"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6248325.1"
                     /translation="MAYDYELLDVDIDGRVATVTVSNPPINLITVALYRELASLGAEL
                     RADPNLTVVVMKSADPDFFLAHFDVETILSFPVDGEAQRSAELSEYHLMCERFRTMDK
                     VTIAQIEGRVGGGGSELAASFDMRFGVRGRTKINQMEVPLGILPGGTGTQRLPRLVGR
                     GRALEIVLGGADLDAETAESWGYLNRIFEPEEIGGFVAALARRIASYPVEAVRLAKEA
                     VLGAETPVHQGLSNEAYLFQRLLRTEGAQRNMRRFLQIGGQTREGELRMGELCAELGE
                     ESR"
     gene            3240..4421
                     /locus_tag="AC16UZ_16425"
     CDS             3240..4421
                     /locus_tag="AC16UZ_16425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR6248326.1"
                     /translation="MDGRQQAIVNRIDELADELLAVSHAIHANPELAFEEHDACALLA
                     STARRFDLPVETGVFTLETAFQGSFGGRTSGPTVAILAEYDALPGIGHACGHNLIATS
                     ALGAALGLKAVDDELPGTVRLIGTPAEERGGGKELMARAGAFDGVDAAMMIHPAGVNL
                     ATMPCICVAEVEVIYHGRSAHASAMPHKGINALDGLLLAYQAISNLRQHIRDHERIHG
                     IVTEGGLAPNIVPDRAAGAFYVRAANEQDLARLKPRVQACFEAGAKGSGCELEVNWAN
                     VDYLDLNTNWPLANRFQAHGERLGREFLKPASLGGAGSTDMGNVSYRVPSIHPMLACA
                     PPNVVIHNPEFAKWARSEQGDAAAIDGAKALALTAADYLLDGSLQAEVNKAFAISKGL
                     A"
     gene            4438..4977
                     /locus_tag="AC16UZ_16430"
     CDS             4438..4977
                     /locus_tag="AC16UZ_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HIR6248327.1"
                     /translation="MNVWLFGYGSLIWRPEIAYVERRPARLHGWARRFWQGSHDHRGM
                     PRQPGRVVTLIEEEGGHCDGMAYLVDRGDVEAIFAALDHREKNGYRRFDVELHVSRYN
                     PAPATTGVVYIAPPGNHAFLGPAPLAEMARQISECAGPSGRNVDYLLDLAEALRQLDA
                     QDAHVFELEAAVRHVSGSR"
     gene            4977..6176
                     /locus_tag="AC16UZ_16435"
     CDS             4977..6176
                     /locus_tag="AC16UZ_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6248328.1"
                     /translation="MDISFSAEHERFRDEVRVFLDGALDDELREAQRFCPGIFLDYEH
                     NIRWHRILHAKGWVAPAWPREYGGTGWDLTRRYIWSTETTLAGAPSLAPMGLGMCGPM
                     LIGYGTEAQKAYYLPRILSGEDYWCQGYSEPGSGSDLASLKLRADGDGDHYVLNGTKI
                     WTTHAHYANRMFCLVRTDDGGRPQQGITFLLLDMDTPGISVEPIVFASGTHEVNQVFF
                     DDVRVPKANRVGEENDGWTVAKYLLEFERGGGGSAAYRTALTRVRKLAGQVPADGASL
                     AEDPDFKRALDETDVKLQAIQYTEFRIMAALSRGQNPGPESSIMKNLGADIGQRLTEL
                     TVEAMGQYIAPHQPEARQVGSNVPVVGPADGATAMPRYFNLRASSIAGGTNEVQKNIV
                     AKLVLGL"
     gene            6213..6950
                     /locus_tag="AC16UZ_16440"
     CDS             6213..6950
                     /locus_tag="AC16UZ_16440"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6248329.1"
                     /translation="MSELVLREDREGLAILTLNRPEALNALSPSLFVELRRHVDDIAQ
                     DVDNVGCVLLRGEGRSFSAGNDLKAIQAGERAPGPHFQAETLDAIEGLPQPVIAAVQG
                     HCYTGSLELALACDLIITARGAKIADTHGKWAMSPTWGMSQRLPRRVGLARAKELMYS
                     GRVIDGEEAAAIGLAVRCVPDEELLEAAVAMARTFLENSWFTLRADKMLVNGGLNHTL
                     ADGLAFERSNSPGRGPDLEERLERFGK"
     gene            complement(6970..7560)
                     /locus_tag="AC16UZ_16445"
     CDS             complement(6970..7560)
                     /locus_tag="AC16UZ_16445"
                     /inference="COORDINATES: protein motif:HMM:NF009205.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIR6248330.1"
                     /translation="MTRLDGVEEAAEVAKQFQFLENTLLKNRTLTLFGEINKDVAQRT
                     AEKLLALAYESDDPIVLYIGSPGGHVESGDTIFDMIRYIKPNVKAVGTGWVGSIATHI
                     YLAPDREDRFCLPNTRFLIHQPAGGFGGSASDVEIHAREIVRTRQRINAIIAEQTGQP
                     LERVEEDTDRDYWMSAEESVEYGIVGRIISSIDELG"
     gene            7621..8559
                     /locus_tag="AC16UZ_16450"
     CDS             7621..8559
                     /locus_tag="AC16UZ_16450"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6248331.1"
                     /translation="MPLAPEYQSFLEQLAQVPGPPLHELSPADARAAYRLLRPRDEQI
                     TVAAVNELAAPGPAGSIALRVYRPQGDGPFPMLVNFHGGGWVFGDLDTADTVCRNLCR
                     NTGCLVVSVDYRLAPEHPYPAAIDDAYAATCWLAEHASELEGNGRLAVAGESAGGNLA
                     AAVSLRARDEGGPAIDYQLLFYPVIDADFNRASYEENRSGYLLETATMIWFWDHYVPD
                     RSRRSQAYASPIRADDLGRLPPALILTAEFDPLRDEGNAFAEALKAAGTPAELICCAG
                     LIHDFCGTAHLFESSRKPFEDACAALRRAMSGTPES"
     gene            8604..8891
                     /locus_tag="AC16UZ_16455"
     CDS             8604..8891
                     /locus_tag="AC16UZ_16455"
                     /inference="COORDINATES: protein motif:HMM:NF015735.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR6248332.1"
                     /translation="MLYVMICEDKPDSEALRLANRADHLEWVGGKTAMIRLAGPMLSD
                     DGEHMRGSLFIIQAESADAVRQFNGEDPYTRAGLWGKVTIRPFRQVLPADS"
     gene            8905..9621
                     /locus_tag="AC16UZ_16460"
     CDS             8905..9621
                     /locus_tag="AC16UZ_16460"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6248333.1"
                     /translation="MRKAVILGVGPDLGLGAQLCKRFAGEGLHVMVAGRTDTSVEAVA
                     GDIRANGGAATAVVADATSEDDVRALFDQAGDDLDLAIYNAGNNMPGRIIDMEADYFE
                     NSWRVGCFGGFLFGREAVRRMVPKGAGTLLFTGASASLRGRAKFGAFNSAKGALRNLA
                     QAMAKEYGADGIHVGHVIIDGPIAGDKIIKVFPEYAQRLGSEGMIGIEGIVEGFAFLY
                     RQPRNAWTFEVDVRTSLEKR"
     gene            complement(9692..10099)
                     /locus_tag="AC16UZ_16465"
     CDS             complement(9692..10099)
                     /locus_tag="AC16UZ_16465"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR6248334.1"
                     /translation="MALNASDLNAGDTYTECLVENLSRTQIVMYAGTSGDYNPLHSDE
                     VFTTQIAGYPSVFAHGMLTMGMTGRMLTNYVGDGRLTKYGVRFTNQVWPGDTLNATAT
                     VDEVADGIVKLNVETTNQDGAVVLSGYAEARVD"
     gene            complement(10111..10605)
                     /locus_tag="AC16UZ_16470"
     CDS             complement(10111..10605)
                     /locus_tag="AC16UZ_16470"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAS1-like dehydratase domain-containing protein"
                     /protein_id="HIR6248335.1"
                     /translation="MSVDRFPIEASHIMMFARSIADDNQIYYDEEYAKGTEPGTIIAP
                     PTFAQASAQFDPDYFLRPKIGEPWFGSAKEATGVKREGGGSGGGGGGGLHAEQHFEYH
                     RHLKPGDVLSAEVKPGKTWEKASKRAGKLLFSETITEYRDQNGELVITARGVGVRTER
                     PVDR"
     gene            complement(10714..11493)
                     /locus_tag="AC16UZ_16475"
     CDS             complement(10714..11493)
                     /locus_tag="AC16UZ_16475"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6248336.1"
                     /translation="MSEFENILYEVQGPMGLITINRADKHNAISLATLDELHQAVGLA
                     AADDAVRVLTITGAGDKAFASGSDLSEVLHRDFRKALEPIVQGLADQLERMPKPTIAA
                     INGICMGGGLEVALGCDLRIAVPKARFATPEGKLGIIPGGGATARLPRIVGRGWGMEM
                     LLMGEPVDAERALQIGLVTRVVEPAELIPEARRMADHLAGFAPFVPRTMKAMVHFGME
                     ASLAGALMFEKYAQGALVQTDDKVEGITAFLEKRDPEFKGR"
     gene            complement(11535..12191)
                     /locus_tag="AC16UZ_16480"
     CDS             complement(11535..12191)
                     /locus_tag="AC16UZ_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HIR6248337.1"
                     /translation="MANWKTALVAGLVALGSAGCETAYYGAMEQVGVYKRDILVDRVE
                     DAVEAQQDAKEEFADALEQFASVVAVEPSRLKDTYEELSDAYDSAEDRAERVRDRIDS
                     VEDVSEDLFDEWSEEIELIGNPKLKSASASQLRASQRKYDELIKGMRRAERKMEPVLD
                     TFRDHVLFLKHNLNARAIASLKSELTSIEADVAGLIREMEASIAQSQAFIEEMQLVDG
                     "
     gene            12433..13242
                     /locus_tag="AC16UZ_16485"
     CDS             12433..13242
                     /locus_tag="AC16UZ_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6248338.1"
                     /translation="MTAYTDTAIELLMTYGPKFLLALVVLIIGLWIIKRVVKVTSKAM
                     ERGNAEPTLAKFVASLVSVGLKALLIISVASMIGIETTSFIAILGAAGLAVGLALQGT
                     LANFAGGALILLFRPYKVGDWIDAQGVAGTVHAIQIFNTILKTPDNRTIIIPNGAISN
                     GIITNNSTEPTRRVDFVFGIGYGDDIAKAKGIIERLIGADERTLSDPAAQIVVSALAD
                     SSVNLTVRVWVNAADYWGVFFDLTEKVKLAFDEEGVSIPFPQQDVHMHQAG"
     gene            complement(13485..>13858)
                     /locus_tag="AC16UZ_16490"
     CDS             complement(13485..>13858)
                     /locus_tag="AC16UZ_16490"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6248339.1"
                     /translation="VVASFDQSIPLTSDGDDVRVSVAPGALQRVLINLITNALQHGKG
                     VRVHIAGREVHVLDSGPGIPEEYRERVFQPFFRLDDSRSVATGGSGLGLAIVQQLCQA
                     QGWRVAIQEADNGEIDVFVTL"
BASE COUNT         2497 a         4652 c         4285 g         2424 t
ORIGIN      
        1 acgccgctgg cttacatcgc ggctcacacc gagcgcatcc ggctcggcac cggcgtcatc
       61 cagctcgctg cccgtacgcc ggccaacgcc gccatggcgt tcgccacgct cgatcagctg
      121 gcgggcggca accgtgccat ctgcggtatc ggggtttccg gcccgcagat cgtcgagggc
      181 tggtacggac agccctgggg acggccctac taccggatca aggattacgt ggccatcatg
      241 aagaagatct tcgcccggga agagcccgtc acccacgccg gcaaggagat cagcctgcct
      301 taccagggcg agggcgccct ggggatcggc aaaccgctga agtcgatcct ccacatgaac
      361 ccgaacatac ccatcttcct gggaactggc atggagtcca ccgtgcgcct gacggccgag
      421 gtcgccgacg ggtggctgcc gctgggattc gtgcccgaaa ccgcagcgct ctacgatggc
      481 tggatcgatg agggccttgc caaaggcggc aagacgtcgg agcagttcga gcggcaggca
      541 ggtacgtcgg tccaggtgac cgacgacgtg aaaagcgctc tggaccggct caagccaggc
      601 atcgcgctgt acgtcggcgg catgggccac aagaccaaga acttccacaa ggagatgatg
      661 atcaggcgcg gcttcggcga agccgcagag cggatccagg agctctacct ggcaaagcgc
      721 aaggacgagg ccatcgccgc cgttcccgac gagttcgtcg accagggcgc cctggtgggt
      781 cccaaggagc gcatcgccag gcgtttccgg gactgggagg attcgggcct caccggtctg
      841 accatttccg gggacgagga agccgtgcgc ttcatggccg agtgcgcgcg gctgaatttc
      901 gccgccgagt gagcctgcgg cgcctagcgg ccggaagcgc ccgctgagcg ccccgaaccc
      961 agggcgtacc gcagacccac catcatcgag tgggtctgct ggtaggcctc caccgggccg
     1021 ccgtccggcc ccgtcatctt cagatcgctc gaaatccagt accggtattc cgccgacacc
     1081 gtcagccgct gggacacgct gacctctgca cccaggcgaa actcggccgc gtaagattgg
     1141 tccttgtcgt ccacgaacag ctcatcgaga acgtctacgt cgtagtcggc ccacacgagc
     1201 cccgcgccga cacccaggta agggcgggtc cgggcttccg gaaagaactg gtagatgccg
     1261 gacgcgagca ggctggtgga gcggatccgg cccgagccct gcttggaacc gaacctgagc
     1321 ccgaaatcca gaagctcgac ctcgttcttc cgaaacgcgg tttccagctc gaagcgccag
     1381 cgccggctga gcgcgtaacc aaccccgaac gccgccaccg ggcccacgtc cagagcgtcg
     1441 aaattcgtct gaccgggatc gatctcgacg tccacggaga atcccgcgcc cgcaaaggtg
     1501 gtgaggtaga agccccgggg cgaaggccga ggggacgctg ccggccgcga ccgctcgacg
     1561 ccgccggaac ggtagcgcag ctgcagccag cctgaatgaa cggcatgttt cgtatcgagc
     1621 ttggtccctg tgaactcctt cagctttacg gacggcgcct gccagtaacg gtactcgacc
     1681 cccagatcca tccttcgggt caggggcagg ctgatccccc cgatcagctg aaatgcaaag
     1741 ctcgtggcct ggtcgtcgac taaaggatct ctgggctcgg gatcaatctg cgcctctatg
     1801 ctgccatcgg ttatgtccag ttccgtccag gccaacccga cgccagcgcc gaggtaaggg
     1861 cgaaagtccc ttcccaccgc aaactcgcgc accgcgttga tcgccaacgt cgtcgcggaa
     1921 aaagcgttct cgggagcggg gtcgagggcg gcggcagacg ccgataccgg cgcggaaaac
     1981 gacacgagct ccacgtcgtt gcgctgccgc gacgcctcga cttccacccg ccagtccggc
     2041 ccgaaagccc aacccagcgc cgcagaccag accggagcgc cgaagtcgta gtcgacgacg
     2101 ccgcccaact cgtccagctc catgttctcg gcgtgattcc agccgatccc cacgcccgca
     2161 taccagccgc cagcctggtc cgcggcagca caggagctcc aaaggagggc acaccccagc
     2221 atggacgcca gagcgccctg gcaccagtgg cgactcacgc cgggatcgac cggcgaccgg
     2281 atggttgccg atacggggtc atcgggattc ctcgccaagc tctgcacaca gctcgcccat
     2341 ccgcagctcg ccttcccggg tttgcccgcc gatctggagg aacctgcgca tgttgcgctg
     2401 ggcgccttcc gtgcgcagca gccgctgaaa caggtaggct tcgttcgaga gcccttggtg
     2461 aacgggggtc tcggccccca gaacggcctc tttcgccagc ctcacggcct ccaccgggta
     2521 cgacgcgatc cgtcgcgcaa gcgccgccac gaacccgccg atttcctccg gctcgaatat
     2581 gcggttcaga tagccccagc tttccgccgt ctcggcatcg agatcagcgc cgcccagcac
     2641 gatctccagc gcgcgccccc gacccaccag cctaggcaga cgctgtgttc cggtcccgcc
     2701 agggagaatg cccaggggca cctccatctg gttgatcttc gttcgaccgc gcaccccgaa
     2761 acgcatgtcg aagcttgctg ccagctcgct tccgccaccg ccgacgcgcc cttcgatctg
     2821 cgcgatggtc accttgtcca tggtgcggaa ccgctcgcac atgaggtggt actccgaaag
     2881 ctcggcggag cgttgggcct caccgtccac ggggaacgaa agaatggttt ccacgtcgaa
     2941 atgcgcgaga aagaagtccg ggtccgcact cttcatcacc accaccgtta ggttcgggtc
     3001 ggcccgaagc tcggcgccga gactcgcaag ctcgcggtag agggccacgg tgatgaggtt
     3061 gatgggcgga ttgctgacgg ttaccgtcgc tacccgccca tcgatgtcca cgtcgagcag
     3121 ctcgtaatcg taagccacgg atatctccct tcctggtgat caaagcgccg cggcattcct
     3181 ctggaggcgt gccgcacgat gccccatact cgagggcatt atatcgacct gaccggaata
     3241 tggacggaag acagcaggcc atcgtcaacc gaatcgacga gctcgccgac gagctgctcg
     3301 ccgtgtccca tgccattcac gccaatccgg agctcgcttt cgaggagcac gatgcctgcg
     3361 cgctgctggc aagcacggcc aggcggttcg atcttccggt ggaaacgggc gttttcaccc
     3421 tggaaaccgc cttccaggga agctttggcg ggcgcacgtc ggggcccacc gtggcgattc
     3481 tcgcggagta cgatgctctg cccgggatcg gccacgcgtg cggccacaac ctcatcgcga
     3541 catccgctct cggtgccgct ctggggctca aggcggtgga tgatgagctg cccgggaccg
     3601 tccgactcat cgggacccct gccgaggaac gcgggggcgg aaaggaactg atggcgcggg
     3661 cgggcgcatt cgacggcgtg gacgcggcga tgatgatcca tcccgcgggg gtgaatcttg
     3721 cgaccatgcc ctgcatctgc gttgccgagg tggaggtgat ctatcacggc cgctctgccc
     3781 acgcctcggc gatgcctcac aagggcatca atgccctcga cggcctcctg ctcgcctacc
     3841 aggccatatc caacctgcgc cagcacatac gcgatcacga gcgcatccac ggcatcgtca
     3901 ccgaaggagg cctggcaccg aacatcgtcc ccgaccgggc cgccggggcc ttctacgtgc
     3961 gagccgccaa cgaacaggat cttgcccgcc tgaagccgcg cgtgcaggca tgtttcgagg
     4021 caggcgcaaa agggagcggc tgcgaattag aggtcaactg ggcgaacgtc gactacctgg
     4081 acctgaacac caactggcct cttgccaacc gcttccaggc ccacggcgag cgtctggggc
     4141 gcgagtttct caaacccgct tccctcggcg gcgcgggcag caccgacatg gggaacgtca
     4201 gctaccgggt accatccata catccgatgc tggcctgtgc tccacccaac gtcgtcatcc
     4261 acaacccgga gttcgccaag tgggctcgtt ccgagcaggg cgacgccgcg gccatcgacg
     4321 gcgcgaaagc cctggctctg accgccgccg actacctcct ggatggctcg ctgcaggcgg
     4381 aggtgaacaa agcgttcgcg atctcgaagg gcctggcctg accgcccgcc cggcctgatg
     4441 aacgtctggc tgttcggcta cggatccctc atctggcgcc cggagatcgc ttacgtggag
     4501 cgccgccccg cccgcctcca cggctgggcg cgccgcttct ggcagggctc ccacgaccac
     4561 cgcggcatgc cccgccagcc cggcagggtg gtgaccctca tcgaggagga aggcggtcat
     4621 tgcgacggca tggcctatct cgtggacagg ggcgatgtcg aagccatatt cgcggcgctg
     4681 gatcatcgag agaagaacgg ctatcggcgc ttcgacgtgg aactgcatgt ctctcgctac
     4741 aatcctgcgc cggccaccac gggagtggtc tacatcgcgc ccccgggcaa tcacgctttc
     4801 ctgggacccg ccccgcttgc ggagatggct cgccagatca gcgagtgcgc ggggccgagc
     4861 ggccgcaacg tcgactacct gctcgacctg gccgaagcgc tgcgtcagct tgacgcccag
     4921 gacgcccatg tgttcgagtt ggaagcggcc gtccggcacg tatccgggag cagataatgg
     4981 acatttcctt cagcgcggag cacgaacgct tccgcgacga ggttcgggtt tttctcgacg
     5041 gggccctgga cgacgagctg cgcgaagccc agcgcttctg ccccggcatc ttcctggatt
     5101 acgagcacaa catccgctgg caccgcatcc tgcacgccaa gggctgggtc gcccccgcgt
     5161 ggccccggga atacgggggc accggctggg acctgacgcg ccggtacatc tggtccacgg
     5221 agaccaccct tgccggcgca ccctcgctcg cgccgatggg ccttggcatg tgcggcccga
     5281 tgctgatcgg ctacggaacc gaagcccaga aagcttatta cctcccgcgg attctctccg
     5341 gcgaggacta ctggtgccag ggctactcgg agccgggttc cgggtcggat ctcgcctccc
     5401 tcaagctccg ggcagacggc gacggcgacc actacgtcct gaacggaacc aagatctgga
     5461 ccacccacgc gcattacgcc aatcgcatgt tctgcctcgt cagaaccgac gacgggggac
     5521 gtccccagca ggggatcacg tttctgcttc tcgacatgga cacgccgggc atcagcgtgg
     5581 agcccatcgt gttcgcatcg ggcactcacg aggtcaatca ggtgttcttc gacgatgtca
     5641 gggttcccaa ggccaaccgt gtcggtgagg agaacgacgg ctggaccgtg gccaagtacc
     5701 tgctcgagtt cgaacgaggt ggcggcggca gcgccgccta ccggacggcc ctgacccggg
     5761 ttcgcaaact ggccggccag gttcccgccg atggcgcttc cctggccgaa gacccggatt
     5821 tcaagcgggc gctggacgaa accgacgtaa agctgcaggc cattcaatac accgagtttc
     5881 gcatcatggc cgcgctctcc cggggccaga accccgggcc cgaatcctcc ataatgaaga
     5941 acctgggcgc cgacatcggc cagcgcctca ccgagctgac cgtcgaagcc atgggccagt
     6001 acatcgcgcc ccatcagccc gaggcccggc aggtgggcag caacgtcccc gtggtaggac
     6061 ccgcggacgg cgcaaccgcc atgccgcgct acttcaacct gcgcgccagc tccatcgctg
     6121 gaggcaccaa cgaggtgcag aagaacatcg tcgccaagct ggtcctcggc ctgtgaacgg
     6181 ggcgctggcc gacaactctc aagggaagtg acatgagcga actcgtactg cgggaggacc
     6241 gcgaaggtct ggcaatcctc accctgaaca gaccggaagc cttgaacgcg ctgagcccca
     6301 gcctgtttgt ggagctgcgc cgccacgtgg acgacatagc gcaagatgtc gacaacgtgg
     6361 gctgcgtgct gctgcgcggc gaaggaaggt ccttctccgc cgggaacgac ctgaaagcca
     6421 ttcaggccgg ggagcgcgcg ccgggcccgc acttccaggc cgaaacgctg gatgccatcg
     6481 aggggctgcc acagccggtc atcgcggccg ttcagggcca ttgctacacg ggctcgctgg
     6541 agcttgcgct ggcctgcgat ctcatcatca ccgcccgcgg cgcgaagatt gcggacaccc
     6601 acggcaagtg ggcaatgtca ccaacctggg ggatgagcca gcgcctgcct agaagagtcg
     6661 gcctggctcg cgccaaggag ctcatgtaca gcggccgcgt catcgacggt gaggaggcgg
     6721 ccgccatcgg tctagcggtg cgctgcgtgc cggatgagga actgctcgag gcagccgtcg
     6781 ccatggcccg cacgttcctg gagaactcct ggttcacgct gcgagccgac aagatgctgg
     6841 tgaacggcgg gttgaaccac acgctagccg acggccttgc gttcgaacga agcaacagcc
     6901 caggcagggg gcccgatctc gaggagcggc tcgagcgctt cggcaagtga gaccggtggt
     6961 ggcccgtcgt cagccgagct cgtcgatgct cgagatgatt cgacccacga tgccgtactc
     7021 caccgactct tcggcgctca tccagtagtc gcggtcggtg tcctcttcca cccgttcgag
     7081 gggctggccc gtctgctccg cgatgatggc gttgatgcgc tgacgggttc tgacgatctc
     7141 gcgggcgtgg atctccacgt ccgatgcgct cccgccaaag ccgcccgcag gctgatggat
     7201 gagaaaccgg gtgttgggga ggcagaagcg gtcctcgcgg tcgggcgcga ggtagatgtg
     7261 cgttgcgatg ctcccgaccc agccggtgcc cacggctttc acgttcggct tgatataccg
     7321 gatcatgtcg aaaatcgtat cgcccgactc cacgtgccca ccgggggatc cgatgtagag
     7381 cacgatgggg tcgtccgact cgtatgccag cgccagaagt ttctccgccg tgcgctgggc
     7441 gacgtccttg ttgatctccc cgaacagggt caaggttcgg ttcttgagca gcgtgttctc
     7501 cagaaactga aactgcttgg ctacctccgc cgcttcttcc acgccatcaa gcctcgtcat
     7561 ccggtgtctc cagatatctt tcggtttaaa cttggggcgc attctacacg ggggatacga
     7621 atgccgttag cgccggaata ccagagtttt ctcgagcagc tcgcccaggt gccgggccct
     7681 cccctgcacg aactgtcgcc cgccgatgcc cgcgccgctt accgcctctt gcggccgagg
     7741 gacgagcaga tcaccgtcgc cgcggtgaac gagctggccg cgcccgggcc agccggatcc
     7801 attgcgctgc gcgtctaccg tccccaggga gatggcccgt ttcccatgct ggtgaacttt
     7861 cacggcgggg gctgggtatt cggcgacctc gataccgccg ataccgtctg ccgcaatctc
     7921 tgccgaaaca cggggtgcct ggtcgtctcc gtggattacc ggctggcgcc cgagcatccc
     7981 taccccgcgg ccatcgacga cgcgtacgcc gccacttgct ggctggcgga acacgcgagc
     8041 gagctggagg gcaacggccg cctggcagtg gccggcgaaa gcgcgggcgg aaatctggcc
     8101 gccgccgtga gcctgcgcgc acgggacgaa ggcggtccgg ccatcgacta tcagctgctc
     8161 ttctatcccg ttatcgatgc ggacttcaac cgggcctcct acgaggagaa ccggagcggc
     8221 tacctgctcg agacggcaac gatgatctgg ttctgggacc actacgtgcc cgataggtca
     8281 cgccgttctc aggcgtacgc ttcgcccatt cgggcagacg atctgggccg cctgccgcca
     8341 gcgctgatcc tgaccgcgga gttcgacccg ctccgggacg aaggcaacgc tttcgccgag
     8401 gcgttgaaag ccgcgggcac gccggcagag ctgatctgct gcgccggcct gattcacgac
     8461 ttctgcggca ccgcgcatct tttcgagtcg agccgaaagc cgttcgagga tgcctgtgcc
     8521 gccctgcgcc gggccatgag cggcacgccc gaaagctgaa gccgaacacc cccgcaaaac
     8581 tgaagcctga ccgaggaaga agcatgcttt acgtgatgat ctgcgaagac aaaccggaca
     8641 gcgaggcgct gcgtctggcg aaccgggccg accatttgga atgggtgggc gggaagacgg
     8701 cgatgatacg gctcgccggc cccatgctga gcgacgacgg tgagcacatg cgcggatccc
     8761 tgttcatcat ccaggccgag tcagccgatg cggtgagaca gttcaatggg gaggatccct
     8821 acacgcgcgc ggggctctgg ggcaaggtga ccatccggcc cttcagacag gtcctgccag
     8881 cagattcatg aggcgaccca gaccatgaga aaagccgtca tcctcggcgt cgggcccgac
     8941 ctcgggctcg gcgcccagct gtgcaaacga tttgccggcg agggtctcca cgtgatggtc
     9001 gccggacgca cggatacttc cgtggaggca gtggccgggg acatccgcgc caatggcggc
     9061 gccgccaccg ccgtagtggc ggacgcgact tcagaggacg atgtccgggc ccttttcgac
     9121 caggccggcg acgacctcga cctggccatc tacaacgccg gcaacaacat gcccggccgc
     9181 atcatcgaca tggaagccga ctacttcgag aacagctggc gcgtgggctg ctttggcggt
     9241 ttccttttcg gccgggaggc cgtccgccgc atggttccaa aaggggccgg gaccctgctg
     9301 ttcaccggtg cgagcgcttc gctgcgcggt cgcgccaagt tcggcgcctt caactcggcc
     9361 aagggcgccc tacgcaatct ggctcaggca atggccaagg agtacggggc agacggcatc
     9421 cacgtgggtc acgtgatcat cgatggaccc atcgcgggag acaagataat caaagtcttc
     9481 ccggagtacg cgcagaggct ggggtcggaa ggcatgatcg gcatcgaagg tatcgtcgag
     9541 ggcttcgcct tcctgtaccg ccaaccgcgc aacgcctgga cgttcgaggt ggacgtgcgc
     9601 acttccctgg agaagaggta acccgggcag accccctgag cccatcggcg agaccacgaa
     9661 ggttcaccgg gcctcatggt ctctgccccg ttcagtcgac gcgggcttct gcgtaaccgg
     9721 aaagcaccac cgcgccatcc tgattggtgg tttccacgtt cagcttcacg atgccgtcgg
     9781 ccacttcgtc caccgtggcg gtcgcgttca gcgtgtcgcc cggccagacc tggttggtaa
     9841 agcgcacgcc gtacttggtg agccgtccgt cccccacgta gttggtcagc attcggccgg
     9901 tcatgcccat ggtcagcatc ccgtgggcga aaaccgacgg gtagccggcg atctgcgtcg
     9961 tgaacacctc gtcggagtgc agcgggttgt aatcccccga tgtgccggca tacatgacga
    10021 tctgggtccg gctcaggttc tcgacgagac actcggtgta ggtgtcgccg gcgttgaggt
    10081 cgcttgcgtt cagtgccatc tctccctccc ttatcggtcc accgggcgct cggtgcgcac
    10141 acctacgccg cgggcagtga tcaccagctc accgttctga tcccgatact cggtgatcgt
    10201 ctcactgaac agcagcttgc ccgcccgttt gctcgccttc tcccaggtct tgccgggctt
    10261 tacctccgcg ctcaggacgt cgccgggctt caggtgccgg tgatactcga agtgctgctc
    10321 ggcatgcagg ccgccgcctc caccgccccc gctgccgccg ccttcgcgct tgaccccggt
    10381 ggcttctttg gcagagccga accagggctc accgatcttc ggccggagga aataatccgg
    10441 atcgaactgg gcgctcgcct gggcgaacgt cggtggggcg atgatggtgc ccggctcggt
    10501 tcctttcgcg tactcctcgt cgtagtagat ctgattgtcg tcggcaatcg agcgtgcaaa
    10561 catcatgatg tggcttgcct cgatcgggaa tctgtccacg gacatcgtcg tttccccctc
    10621 gtgttcgttg tgcgcgttgg cgctctaccc ctcgccgata tacggcgtct cagagcaaag
    10681 gagcggaagt atcccccaat ggtctggggg ggatcaacga cccttgaatt ccgggtcgcg
    10741 cttctccaga aaggccgtga tgccttctac tttgtcatcg gtctgaacca acgccccctg
    10801 ggcatacttc tcgaacatga gcgcgccggc caggctcgcc tccatgccga aatgcaccat
    10861 cgccttcatc gtgcggggca cgaagggggc gaagccggcc aggtggtcgg ccatgcggcg
    10921 tgcctcaggg atcagctcgg ccggctcaac gactcgcgtc acgagaccta tctgcagagc
    10981 cctttcggca tctaccggct cgcccatgag cagcatctcc atcccccagc ccctccccac
    11041 gattcggggc aggcgggccg tggccccgcc accggggatg atgcccagct ttccttcggg
    11101 ggtcgcaaag cgcgccttcg gcaccgcgat cctcaggtca cagccgagag cgacttccag
    11161 acctccaccc atgcagatcc cgttgatggc cgcgatggtc ggcttcggca tgcgctccag
    11221 ctggtccgcc aggccctgca cgatgggctc gagcgccttc ctgaaatcgc ggtgcaggac
    11281 ctcagagagg tcggatcccg aagcaaaggc cttatccccg gcgccggtga tggtcagcac
    11341 cctcaccgcg tcgtccgccg cggcgaggcc caccgcctga tgcagctcgt ctagggtggc
    11401 cagggagatc gcgttgtgct tgtccgcccg gttgatggtg atgagcccca tggggccctg
    11461 gacttcgtag aggatgttct cgaattcgct cacgtgcctg gttccgttgt ttggcccatc
    11521 gtctgctgcg cgggtcagcc gtcgaccagc tgcatctctt cgatgaaggc ctgggattgc
    11581 gcaatggacg cttccatctc gcggatgagg cccgccacgt ccgcttctat ggaggttagc
    11641 tcgcttttca gggacgcgat ggcccgggcg ttgagattgt gcttcaaaaa cagaacgtgg
    11701 tcccggaaag tgtccagcac cggctccatt ttccgttccg cccgccgcat tcccttgatc
    11761 agctcgtcgt actttcgctg tgaggctcgc agctggctgg cgctggcgct tttgagcttc
    11821 gggttgccga tcagctcgat ctcctcactc cactcgtcga aaagatcttc cgagacgtct
    11881 tcgaccgaat cgatacggtc ccgaacccgc tcggctcgat cctccgccga atcgtaggca
    11941 tccgacagtt cctcgtaggt gtcctttagt ctcgatggct ctacagcaac cacgctggcg
    12001 aactgctcta aagcgtctgc gaactcctcc ttggcgtctt gctgggcttc cacggcgtcc
    12061 tcgacgcgat ccacgagtat gtcgcgcttg taaacgccaa cctgctccat cgcaccgtaa
    12121 taggccgttt cgcagcctgc tgaccccagc gctacgagcc cggctacaag cgccgttttc
    12181 caattcgcca ccagccttct cccaacccga tacaaaccct ttccacgacc cgaccaatct
    12241 caacgaactc gggaacacgg tgctgaacgc tcaggtcggg ctgttgtttc tcgcgccgat
    12301 ccttaggaca atcgcgtccc aaatcaggcg ctcaacgcag aagcgcgcgc atagcgtgcc
    12361 cgagccccat caaaacaaca accactgaaa cacgggaggc cacaccccat ggaggagatt
    12421 tccacagagc agatgactgc ttacaccgac accgccatcg agctgctgat gacctacgga
    12481 cccaagttcc tgttggcgct cgtcgttctc atcatcggcc tgtggatcat caagcgcgtc
    12541 gtcaaagtca cgtcaaaggc gatggagcgt gggaacgccg agcccaccct tgcgaagttt
    12601 gtcgcaagcc tcgtctccgt aggcttgaaa gccctgttga tcatcagcgt ggcatcgatg
    12661 atcggcatag agaccacgtc cttcatcgcc attctcggtg ccgccggcct ggccgtcggt
    12721 ctggctctgc agggcaccct cgcgaatttt gcgggcggcg ctctgatcct gctctttcgc
    12781 ccttacaagg ttggtgactg gatcgacgct cagggcgttg caggaacggt gcacgccatt
    12841 cagattttca acacgattct gaagacaccg gacaaccgga ccatcatcat cccgaacggc
    12901 gccatatcga acggcatcat caccaacaac tccaccgagc cgacccgccg cgtggacttc
    12961 gtgttcggca tcggctacgg ggacgacatt gccaaagcca aaggcatcat cgagaggctg
    13021 atcggggccg acgagcggac cctttccgac cccgcggctc agatcgtggt ttccgcgctc
    13081 gccgacagct cggtcaacct caccgtaagg gtctgggtca acgccgccga ttactggggc
    13141 gtgttcttcg atctcaccga gaaggtcaag ctcgcattcg acgaggaggg agtttccatt
    13201 ccgttccctc agcaggacgt gcacatgcac caggcaggct gacgctcgct ggggtgtttt
    13261 cgggcgccgc ctgaccagcg gcgctcgagc aaaaaaaaac gccggcttgg agagaaagcc
    13321 ggcgtctaaa ggacctttta caaggtctca aggggataga gattgcctca tggggaagcg
    13381 gtttaacccg cttcgaggtc caatatgagg gaaagacgtt tcgccgcgat ttcctgccgc
    13441 gacctttttg tgacattttg tttcccgatg cccaccggtt cgacctagag cgtaacaaag
    13501 acgtcgatct cgccgttgtc cgcttcctga atggccactc gccagccctg cgcctggcaa
    13561 agctgctgga cgatggccag cccgaggccg ctgccacccg tggctacact gcgtgaatcg
    13621 tcaaggcgaa agaaaggctg aaagacccgc tcccggtact cttcggggat gcccggcccg
    13681 ctgtcgagga cgtgcacctc ccggccggca atgtggacgc gcacgccctt gccgtgctgc
    13741 aaggcgttgg tgatcaggtt tatcaacaca cgctgcagcg cgcccggggc gacgctgacg
    13801 cgaacgtcgt ctccgtcact ggtcagcggg atggactggt cgaagctggc aaccacat
//
LOCUS       DBNHOX010000759         4845 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1347655, whole genome shotgun sequence.
ACCESSION   DBNHOX010000759 DBNHOX010000000
VERSION     DBNHOX010000759.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1347655"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..953
                     /locus_tag="AC16UZ_16495"
                     /pseudo
     CDS             54..953
                     /locus_tag="AC16UZ_16495"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
     gene            complement(1215..3308)
                     /gene="dinG"
                     /locus_tag="AC16UZ_16500"
     CDS             complement(1215..3308)
                     /gene="dinG"
                     /locus_tag="AC16UZ_16500"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008729.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HIR6248340.1"
                     /translation="MSEALLNDELKGVIQTGYRAWLDARGFRPRRGQREMIALIARTL
                     TGDAPRIVAVEAGTGTGKTAAYCLAAIPTGAALDKTVVISTATVALQEQVVLRDLPDL
                     ASRSGLTFSFTLAKGRGRYVCLKRLDDRLRFAEPSEIPLFEDTGEEGAALYQAMLNAF
                     ANKTWNGDRDEWTEEVDPQLWRAVTTDHRGCSNNRCGFFRQCPFFKARGALDAADVIV
                     ANHDLVLADLSLGGGVVLPPPEDCIYVLDEAHHLPEKTQQHFSASTRVLATGQWVDNV
                     ARVIGSLTQRFGRPDELVSLASRASEHGAAFAEAMMRMGDDLQGLSFEPRGEGLATHR
                     FRLGQVPAELAEQAQLALEPLTLLAGVISAVHELLQKAVAGELPWDNGFEAEDWLPAV
                     GQLETRALATMALLEDFGRGAAEFSKDGSPQGLKARWVNETAGDLEMVSAPLQPGQLL
                     QEALWSSCYAAVCTSATLTALGTFDRFLEQVGLGSVNAVRIPSPFDFQRLATLTVPSM
                     RSDPREFEAHSQEVAALLPGLLADDPGALVLFTSWRQMHEVIGQLPEDLLDKLQVQGE
                     ASKQALLSRHRSAVDEGRQSVLVGLASFAEGVDLPDDYCRHVVIVKLPFAVPDDPMDQ
                     AMAEWVEARGRNAFFEIAVPDAALKLVQACGRLVRHEGDFGRITLLDRRIVTARYGRA
                     LLDSLPPYRRELNPS"
     gene            complement(3354..3971)
                     /locus_tag="AC16UZ_16505"
     CDS             complement(3354..3971)
                     /locus_tag="AC16UZ_16505"
                     /inference="COORDINATES: protein motif:HMM:NF039960.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR6248341.1"
                     /translation="MGRARVARAAVLGLSVVVMAGCASAPPSKPDQICSIFREKSGWY
                     KDARRAEKRWDMPISVGMAFVYKESSYRSDARPERERLLWVVPWRRPSSAFGYAQITD
                     AAWREYLDDTRGFLRERDDFGTALDFVGWYNDRSHRRLGIAKNDAYNLYLAYYVGHGG
                     YKRGHHRKKPEVRRYARRVSEKSKRYAAQLSRCEKEFRKPWWRPI"
     gene            complement(3964..>4845)
                     /locus_tag="AC16UZ_16510"
     CDS             complement(3964..>4845)
                     /locus_tag="AC16UZ_16510"
                     /inference="COORDINATES: protein motif:HMM:NF012743.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR6248342.1"
                     /translation="GDVIPQVVSVIDSRRPPGAEAVALPQGCPSCGSPITRPDDEAVA
                     RCSAGPEICPAQRKEGLRHFASRLALDIEGLGDKLIEQLVDKDQVRIAADLFSLRKDE
                     LIRLERMGAKSAENLLAAIERSRETTLARFIYALGIREVGEATAQSLARHFGDLEPLR
                     RAQVAELEQVPDVGPVVAGNIHSFFANDDGARAVDALLEAGIRWPAVAVETGAQPLLG
                     QTWVLTGTLESMTRDAAKSELVALGAKVSGSVSGKTSQVVAGPGAGSKLEKARDLGVP
                     VMDEAEFLEVLESHRHG"
BASE COUNT          819 a         1723 c         1532 g          771 t
ORIGIN      
        1 accgcgatac cgagtttggc acgccacggg ccaacgtcta cctgctgctg ggcctgcgtg
       61 acgggctcga gtccccccgc gacatcgcca tggcccagct ctatcagcgc ctcgtcaacg
      121 acgccagcaa ccagctggcc tacccggcct ggctcgcggg cctgagctac cgactgtctc
      181 tcgcacccca gggttttcag ctcatcatct cgggttacag cgacaagcag gccgccctgc
      241 tcgacgcggt gatcgaaaac ctcaccgacg tacccatcga tgccgcaaag ttcgagctct
      301 accaggcgga actggtgcgg gagtggcgca acttcgccca ggaacgtccg gtcacccaga
      361 cctacagcgc gctctggttt ctgctgctgt cgagcagctg gcctcccgag gcgctcgcat
      421 ctgccctgga gaacgcaaag ccggaggacc tggcggcctg gcgcagcaag aggctcgacg
      481 ttttcgacgt gaccggcctg gcgcacggaa acgtggacgc tgccgccatc gacgacatcg
      541 ccgatctgat ccgcgagcac ctgccccagc agggtttcgg cctcagccag ccccaggtcc
      601 gcaccgttgc cgagtcactg aagctcgcgg tccccgtgga ccacccggac gcggcgatga
      661 ttcttcacgt gcaggatccg gacaccagct acgaggcgcg cgcccgcagc gtgatggccg
      721 cgcacctcct gagcgaaccg tacttcacaa gcctgcgcac ggagcagcag ctcggctacg
      781 tggtggacat caccgctcgg gcgctccgca gacggggcgc catcaccttc ctgatccagt
      841 caccggtggc aagcccggct cagctcgagg cggccacccg ccagttcgtc accgatcagc
      901 tggccgccct gcaggacatg ccgaccgagc agttcgagga gagccgggcg ggcctcatcg
      961 cgcggttgac cgagcaggac agcaacctcg gccagcgcag ccagcgctac tgggcggata
     1021 tcgaaacggg cgtaaccagc ctcgactccc gggaaaggat cgcagaggcc gtgaacggcc
     1081 tcgccgtgag cgacatgatc gcctatctga agaaccttca gcagaagctg gacgaccagc
     1141 gactctcatt tatcacgacg gccaatttcc gccgggcccc cttggcggca gggagatcga
     1201 gagcgtccag gccttcaaga ggggttgagc tcccgcctgt agggaggcag cgagtcgagc
     1261 agggcacgcc cgtaacgcgc cgtgacgatg cgtctgtcga gcaacgtgat ccggccgaag
     1321 tctccttcgt gtctgaccag gcgcccgcat gcctggacca gcttgagcgc cgcgtccggc
     1381 accgcgatct cgaagaacgc gttgcgcccg cgggcttcga cccattcggc catggcctga
     1441 tccatcggat cgtccggcac ggcaaagggc agtttgacga tgaccacgtg ccggcagtag
     1501 tcgtcgggca ggtcgacacc ctcggcgaag ctggcaaggc cgaccaggac gctctgccga
     1561 ccctcgtcca ccgcgctgcg gtgccgggat agaagcgcct gcttggaggc ctcgccctga
     1621 acctgcagct tgtccagcag gtcctccggg agctgtccga tcacctcgtg catctgccgc
     1681 caggacgtga acagcaccag cgcgcccggg tcatcggcaa gcaatcccgg caggagcgcg
     1741 gccacctcct ggctgtgcgc ctcgaactcc ctggggtccg agcgcatgct cggcaccgtc
     1801 aacgtcgcca gccgctgaaa gtcgaatggg ctcggaatcc ggaccgcgtt gaccgaaccc
     1861 aggcccacct gctccagaaa gcggtcgaag gtgcccagag cagtcagcgt cgccgaggtg
     1921 cacacggccg cgtagcaact cgaccagagc gcctcctgca gaagctggcc gggctgcagc
     1981 ggggcgctga ccatttccag atcacccgcg gtctcgttga cccagcgggc cttcaatccc
     2041 tggggcgagc cgtcttttga aaactctgcc gcgccgcggc cgaaatcctc cagcagagcc
     2101 atggtcgcaa gcgcgcgcgt ttccagctgc cccacggccg gcagccagtc ctccgcctcg
     2161 aagccgttgt cccagggcaa ctcgccggca accgctttct gcagcagctc gtggacggcg
     2221 gagatcacgc cggcaaggag cgtgagcggc tccagcgcca gctgcgcctg ctctgccagc
     2281 tccgctggta cctgtcccag cctgaagcgg tgcgttgcca ggccttcacc gcgcggttcg
     2341 aagctgagcc cctggagatc gtcccccatg cgcatcatgg cttcagcgaa cgcggcgccg
     2401 tgctccgagg ccctgctggc gagactgacg agctcgtccg gtcgcccgaa acgctgcgtc
     2461 aagctgccga tgacccgggc aacgttgtcc acccactgcc cggtggcgag gacccgcgtg
     2521 gaggccgaga aatgctgctg ggttttctcc ggcagatggt gcgcctcgtc gagaacatag
     2581 atgcagtcct ccggcggcgg cagaacgacg ccgccaccga ggctgaggtc ggccagcacc
     2641 aggtcgtggt tggcgacgat gacgtcggcg gcgtcgagcg ctcccctggc cttgaagaac
     2701 gggcactgcc gaaagaagcc acaccgattg ttgctgcatc cccgatggtc cgtggtcacg
     2761 gcccgccaca gctgcgggtc gacctcctcg gtccactcgt cgcgatcgcc gttccaggtt
     2821 ttgttcgcaa acgcgttgag catggcctgg taaagggccg cgccctcctc gcccgtgtcc
     2881 tcgaacaggg ggatctcaga gggctccgca aatcgcagcc gatcgtcgag ccgcttcagg
     2941 cagacgtagc gcccccgccc tttggccagg gtgaaactga acgtcaggcc agaacggctc
     3001 gccagatcgg ggaggtcgcg caggaccacc tgctcctgca gggccaccgt cgcggtgctg
     3061 ataaccaccg tcttgtccag agcagcgccc gtggggatgg ccgccaggca ataggccgcc
     3121 gtcttgcccg tcccagtgcc cgcctccacg gccacgatgc gcggcgcgtc accggtcagc
     3181 gtccgggcga tcagcgcgat catctcccgc tggccgcgtc ggggccgaaa gcccctcgcg
     3241 tcgagccagg cacggtatcc ggtctgaata acgcctttca gctcgtcgtt gaggagggcc
     3301 tcgctcatcg agcgagctta cgttgccggt cgatcactgg ccaccggcaa cgctcaaatc
     3361 ggccgccacc agggcttgcg gaactctttc tcgcagcgcg acagctgcgc cgcgtatcgc
     3421 ttgctcttct ccgagacccg tcgagcataa cggcggacct ccggcttctt ccggtgatgt
     3481 ccccgcttgt agccgccatg gccgacgtag taggcgagat agagattgta ggcatcgttt
     3541 ttggcgatac cgagacgccg gtgactgcgg tcgttgtacc agccgacgaa atccagggcc
     3601 gtgccgaagt cgtcgcgctc gcgcaggaac cctctggtgt cgtcgaggta ttcccgccag
     3661 gcggcatcgg tgatctgtgc gtaaccaaac gccgaggacg gccgccgcca gggcactacc
     3721 cacaacaggc gctcacgctc cggcctcgcg tcggaccggt aggacgactc cttgtagacg
     3781 aacgccatgc ccacggagat gggcatgtcc cagcgcttct cggcacgcct ggcgtctttg
     3841 taccaaccgg atttttcccg gaagatcgag cagatctgat ccggcttcga cggcggcgcg
     3901 ctggcgcacc cggccatcac gaccacgctc aagccgagaa cggcggcccg agcgacgcga
     3961 gccctaccca tggcggtggc tctccaggac ctcgagaaac tcggcttcgt ccatcaccgg
     4021 cacccccagg tcgcgcgcct tttccagctt gctgccggcg cccgggccgg ccaccacctg
     4081 ggacgtcttc cccgacaccg agccgctcac cttggcgccc agggcaacga gctccgactt
     4141 ggccgcatcc ctggtcatgc tctccagcgt gccggtcagc acccaggtct gcccgaggag
     4201 cggctgcgcg ccggtctcga cggctaccgc cggccaccgg attcccgcct ccagcagcgc
     4261 gtctacggcc cgggcgccgt cgtcgttggc aaagaagcta tgaatgttgc cggcgaccac
     4321 cgggcccacg tccggcactt gttcgagctc ggctacctgc gcccgccgaa ggggttccag
     4381 atcgccgaaa tgacgggcca ggctctgggc agtcgcttcc cccacctcgc gaatgcccag
     4441 agcgtagatg aaacgcgcaa gcgtggtctc gcggctgcgc tcgatggcgg ccagcaggtt
     4501 ctcggccgac ttggcgccca tgcgctccag acggatcagc tcgtccttgc gcaggctgaa
     4561 gagatccgca gcgatcctca cctgatcctt gtccaccagc tgctcgatga gcttgtcgcc
     4621 gaggccctcg atgtccagtg ccagacggct ggcaaagtgg cgcaggccct ctttgcgctg
     4681 ggcgggacag atctcggggc cggccgagca gcgcgcaact gcctcgtcgt ccgggcgcgt
     4741 gatgggactg ccacacgacg ggcacccctg aggcagcgcc accgcttcgg cgccgggcgg
     4801 gcgtctggaa tcgatgacgc tgacgacctg gggaatgacg tcgcc
//
LOCUS       DBNHOX010000760         2884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1540979, whole genome shotgun sequence.
ACCESSION   DBNHOX010000760 DBNHOX010000000
VERSION     DBNHOX010000760.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1540979"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="AC16UZ_16515"
     CDS             <1..652
                     /locus_tag="AC16UZ_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248343.1"
                     /translation="TTSNRKTWLFRNTLRYQLNESSRLVGKLNHADSTSSLGDFFAGG
                     YTEAVFGYAYRPVRHDRLNALMKYTYFYNVPTTDQVSLTQTAADFVQKSHIASVDVTY
                     DLTSRWSIGGKYAYRLGEISYDRENPEFFDNTASLYVVRLDWEFRENWEIMAETRLLD
                     LADFQEQRQGYLVAVSRNFGDHFKVGAGYNFTDFSSDLTDLGFDHQGMFLNLTGAF"
     gene            complement(699..>2884)
                     /locus_tag="AC16UZ_16520"
     CDS             complement(699..>2884)
                     /locus_tag="AC16UZ_16520"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6248344.1"
                     /translation="FSDSDIRELGMLRPEDLAAQTPGLDIKNSLGNHNPVFTLRGVGL
                     NDYNVNNNPSVGVYVDEVYMASGAYLSFQIFDMERVEVLKGPQGTLYGRNTTGGAINF
                     LTAKPTDELEAYVDLDYGRWNTLKLEGAVSGPLGDTISGRLAFQMNRSDGYYKNNGNA
                     GSSAGLVAPSSGGLQFLVPIISGGALIPADPQEAIPPNPLVEADDDFFEQDNFALRGT
                     VDWDISDAVDLTVSLHYGEDKGDMLVRSLNGTDTAGFTPADDDPFTVDSNNGVGGPEV
                     DIEAFGGYAKLDADLGFATLTSITGYETIERVLPFEESSPWRLVDQLFMEDMSEFSQE
                     IRLASNDAAQVFWMVGLYYGKEDIEAGKDLNGLDGGLSGFINTSYEQDRETFAAFAHT
                     EWQVAETVRLTGGIRYTADDNRYAGGSFIPEPPLGIYGTNAGGVVIGLPYYDDRSFDE
                     DNVSGKVGIDWTPQEDLLFYLSWNKGYKSGGFDGSTITDESAFTPFRGEDLYAWEAGM
                     KSRWADNRVQLNLTAFLYDFEDMQAEGQREVFPGTFESIRANVGESEIWGIEAELWLK
                     PAAGLDVKLGLAYLDTEITDWAADGLDSDDPDVVADAIADVEAHVGNEVPDSPELTFN
                     GLLRYQRPLSDGLNITGMFNFNFVDDVYKNIDNDEHLRADSYWLLNARLAISSADERW
                     EAAIWGKNLADEFYYRERFDNFGPSWIYETPGAPRSYGVNVVYRW"
BASE COUNT          531 a          938 c          881 g          534 t
ORIGIN      
        1 caccacctcc aaccgcaaga cgtggctgtt caggaacacg ttgaggtacc agctgaacga
       61 aagctcccgc ctcgtgggca agctgaacca cgcggacagc acgagctcgc tgggcgactt
      121 cttcgccggt ggctacaccg aggccgtctt cggctacgcc taccgacccg tgcgccacga
      181 caggctgaac gcgctgatga agtacaccta cttctacaac gttccgacca cggatcaggt
      241 ctcgctcacc cagacggccg cggacttcgt ccagaagagc cacatcgcgt ccgtggacgt
      301 cacctacgac ctcacctcac gctggtccat tggcgggaag tacgcatacc gcctcggcga
      361 gatcagctac gaccgggaga accccgagtt cttcgacaac accgccagcc tctacgtggt
      421 gcggctggac tgggagttcc gcgagaactg ggaaatcatg gccgagacgc gcctgctcga
      481 cctggccgac ttccaagagc agcgccaggg ctatctcgtg gccgtctcgc gcaacttcgg
      541 cgatcacttc aaggtcggcg ccggctacaa cttcaccgac ttctcatccg acctgacgga
      601 tctcggcttc gatcatcagg gcatgttcct caacctcacc ggagccttct agccggagcg
      661 ccccagcgcg ccccggccag cccgatcggc gggttcgact accaccgata gacgacgtta
      721 acaccgtaag agcgaggcgc tccgggggtc tcgtaaatcc agctgggacc gaagttgtcg
      781 aaacgctcgc ggtagtagaa ctcgtccgcc agattcttcc cccatatggc cgcctcccag
      841 cgctcatccg ccgaggagat ggccaggcgc gcattgagca gccagtacga gtccgccctc
      901 aagtgttcat cgttgtcgat gttcttgtag acgtcgtcga cgaagttgaa gttgaacatg
      961 ccggtgatgt tgagaccgtc cgacagcggt cgctggtagc gcaggaggcc gttgaaagtg
     1021 agctccggcg agtccggaac ctcgttgccg acatgtgcct cgacatcggc gatggcatcg
     1081 gccaccacgt cgggatcgtc cgaatccagg ccgtccgccg cccagtcggt gatttccgtg
     1141 tccagatacg ccaggcccaa cttcacgtcg aggccggccg ccggcttcag ccagagctcg
     1201 gcctcgatgc cccagatctc cgactcgccc acgttggcgc ggatgctctc gaacgtaccg
     1261 ggaaagacct cccgctgccc ctcagcctgc atgtcctcga agtcatagag gaacgcggtc
     1321 aagttcagct gcacgcggtt gtctgcccaa cggctcttca tgccggcctc ccaggcatac
     1381 agatcctcgc ctcggaacgg cgtgaaggcc gactcgtcgg tgatggtgga gccatcgaac
     1441 ccgccgctct tgtacccctt gttccagctc aggtagaaca ggaggtcctc ctggggcgtc
     1501 cagtcgatgc cgaccttgcc ggacacgttg tcctcgtcga aggagcggtc gtcgtagtaa
     1561 ggcaggccga tgaccacgcc ccctgcgttg gtgccgtata tgccaagggg cggctcagga
     1621 atgaaggagc ccccggcata gcgattgtcg tctgcggtgt agcgaatccc gccggtcagc
     1681 cggaccgtct ccgcgacctg ccactcggtg tgggcgaaag ccgcaaacgt ctcgcggtcc
     1741 tgctcgtagg acgtgttgat gaagcccgac aagccgccgt ccaggccgtt caggtccttg
     1801 ccggcctcga tgtcttcctt gccgtaatac agccccacca tccagaacac ctgcgctgcg
     1861 tcgttggacg ctaggcgaat ctcctggctg aactccgaca tgtcttccat gaacagctga
     1921 tccaccaggc gccagggact ggactcttcg aaaggcagca cgcgctcgat ggtctcgtag
     1981 ccggtgatgg aggtcagcgt ggcaaacccg agatcggcgt cgagcttggc gtagccgcca
     2041 aaagcctcga tgtcgacctc gggaccgccg acgccgttgt tcgaatcgac ggtgaagggg
     2101 tcgtcgtcag cgggcgtaaa gccggccgtg tcggtcccgt tcagcgaacg cacgagcatg
     2161 tcgcccttgt cctcgccgta gtgcaggctc acggtcagat ccaccgcgtc actgatgtcc
     2221 cagtcgacgg taccccggag cgcgaagttg tcctgctcga agaagtcgtc gtccgcctca
     2281 accagggggt tggggggtat cgcctcctgg ggatccgcgg ggatcagcgc gccgcccgat
     2341 atgatgggga ccaggaactg caacccgcca ctgctgggcg cgacgaggcc ggccgaggaa
     2401 cccgcattac cgttgttctt gtagtagccg tcggagcggt tcatctgaaa ggccagacgg
     2461 ccgctgatcg tatcgccgag cgggccactg acggcgcctt cgagcttcag ggtgttccag
     2521 cggccgtagt cgagatccac ataggcttcc aactcgtcgg tcggcttcgc cgtgagaaag
     2581 ttgatggcgc ctcccgtcgt gttgcggccg taaagcgtgc cctggggtcc tttgagcacc
     2641 tctacccgtt ccatgtcgaa gatctgaaag gagaggtagg cgccgctggc catgtagacc
     2701 tcgtcgacgt aaacgcccac ggacgggttg ttgttgacgt tgtagtcgtt gaggccaacg
     2761 ccgcgcaggg tgaacaccgg gttgtgattt cccagggagt tcttgatgtc gagcccgggg
     2821 gtctgggccg ccagatcctc gggtcgcagc atacccagct cacggatgtc ggagtcggaa
     2881 aacg
//
LOCUS       DBNHOX010000761         3488 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_513396, whole genome shotgun sequence.
ACCESSION   DBNHOX010000761 DBNHOX010000000
VERSION     DBNHOX010000761.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_513396"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="AC16UZ_16525"
     CDS             <1..579
                     /locus_tag="AC16UZ_16525"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HIR6248345.1"
                     /translation="PYLLPRILGPIRKSFPNLKIRLTEGQTAVVGERLRAGDLDAVIL
                     ALPLEEENVVEAELYREPFYFAASKNHPKAGKREVALSDLDQEQVLLLEDGHCLRDQA
                     LEVCNSQNAVENTNFRATSIETLRQMVAADVGITLMPELAIPQKHGAVRYIPFKGNGP
                     HRRIGLCWRGSSSRAALLHDMAGVLREVMAGG"
     gene            complement(607..1188)
                     /locus_tag="AC16UZ_16530"
     CDS             complement(607..1188)
                     /locus_tag="AC16UZ_16530"
                     /inference="COORDINATES: protein motif:HMM:NF023432.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HIR6248346.1"
                     /translation="MQKLNRTDLWSLEQYSEERPAFRAEVIAHKKARRVAIGPHATLC
                     FEDALTMKYQIQEMLRVEKIFEAAEIEEELTTYNPLIPDGANWKATFLIEYPDVEERH
                     RALATMGGIEDTIWVQIGDGPRIYAIANEDMPRSEAGKAAAVHFLRFELDGASVQAAK
                     EGAPILAGIDHASLKGAVTLDPARAESLRRDLD"
     gene            complement(1192..2517)
                     /locus_tag="AC16UZ_16535"
     CDS             complement(1192..2517)
                     /locus_tag="AC16UZ_16535"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR6248347.1"
                     /translation="MKEGSLEAPDRQPLDWQNPQFYDKASLYAELERVYDICHGCRRC
                     VSLCDSFPTLFDLVDESETLEVDGVDKADYMKVVDQCFLCDLCAETKCPYLPPHEWAV
                     DFPHLMLRAKAQKFREKDTKWRDRLITSTDPIFDTLSIPGVAQMANAAAGNSALRKLG
                     DKAVGIHPDAPLPNFSPRPLNKRLDTEIGATLTPKPCERTTGKVAIYITCYGDHNEPQ
                     VVEDLIAVLNHNGIPVKILKDAKCCGMPKLELGDLSKVASLKDKNLPLFLEAIEAGYD
                     IIAPIPSCVLMYKQELPLMFPEDADVEQVKKAFFDPFEYLVLRHKAGLIDTGFGKPLG
                     KVAYHVACHQRVQNIGMKTREFLSLIPDTEVTAIERCSGHDGTYAVKSETYEKAMKIA
                     RPVVNRVKQAEADTFGSDCPMAGRLIAHGMEDDGAAEHPITMVRRAYGV"
     gene            complement(2630..3046)
                     /locus_tag="AC16UZ_16540"
     CDS             complement(2630..3046)
                     /locus_tag="AC16UZ_16540"
                     /inference="COORDINATES: protein motif:HMM:NF014912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HIR6248348.1"
                     /translation="MDLKDSNTLQNLKDAFAGESQANRRYLYFAAKADVEGENDVAAV
                     FRSTAEGETGHAHGHLEYMETVGDPATGLPIGSTGNNLKAAIAGETHEYTDMYPGMAK
                     TARDEGFDEIADWFETLAKAERSHANRFQKALDAMG"
     gene            complement(3243..>3488)
                     /locus_tag="AC16UZ_16545"
     CDS             complement(3243..>3488)
                     /locus_tag="AC16UZ_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248349.1"
                     /translation="GSLLAGFILFLDTRNSRRRGKSGPVSALAIQISIALLSIAGAGA
                     IAFEWLGIPGACAVLAVALFTQVASIVGIARSWRLAT"
BASE COUNT          582 a         1060 c         1132 g          714 t
ORIGIN      
        1 ccctacctgc tgcccaggat cctcggcccc atcaggaagt ccttccccaa cctcaagatc
       61 cggctgaccg aagggcagac ggcggtggtc ggcgagcggc tgcgtgccgg tgacctggac
      121 gccgtgatac tcgcgttgcc cctggaagag gagaacgtgg tggaagccga gctctacagg
      181 gagccgttct attttgccgc ttcgaagaac catccgaagg cgggcaagcg ggaagtagcg
      241 ctcagcgatc tcgatcagga gcaggttctg ctgctcgagg acgggcactg cctgcgcgac
      301 caggctctcg aggtctgcaa ttctcagaac gccgttgaga acacgaactt ccgcgcgacc
      361 agcatcgaga cgctgcgcca gatggtggcg gcggacgttg gcataacgct catgccggag
      421 ctggccatac cgcagaagca cggcgccgtg agatacattc cgttcaaagg caacgggccg
      481 caccgccgaa tcggcctctg ctggcggggc agctccagcc gggcggccct gctgcatgac
      541 atggctggcg ttcttcggga agtcatggcc ggcggctgac ggtgcccgcg cggcgggccg
      601 tccggttcag tccaggtccc gcctcaggct ctctgctctg gccgggtcca gggtcaccgc
      661 tcctttgagc gacgcgtggt cgatgcctgc caggatgggc gcgccttcct tcgccgcctg
      721 cacggaagcc ccgtcgagct cgaagcggag aaaatgcacg gccgccgcct tgcccgcttc
      781 cgaacgcggc atgtcctcgt tcgcgatcgc gtagatgcgc ggcccgtcgc cgatctgcac
      841 ccagatggtg tcctcgatcc cgcccatggt ggccagcgca cggtgccgtt cctccacgtc
      901 cgggtactcg atgagaaaag tcgccttcca gtttgcgccg tcgggaatca gcgggttgta
      961 ggttgtcagc tcctcctcga tctccgcggc ctcgaagatc ttctccacgc gaagcatctc
     1021 ctggatctgg tacttcatgg tgagagcgtc ttcgaagcac agcgtggcat gggggccgat
     1081 ggctacgcgg cgggcctttt tgtgagcgat cacctccgcg cggaacgccg ggcgctcctc
     1141 ggaatactgc tccaggctcc acagatcggt gcggttcagt ttctgcatgg ttcaaactcc
     1201 gtaggcgcga cggaccatgg tgatcggatg ctcggcggcc ccatcatcct ccattccgtg
     1261 cgcgatcagc cgacccgcca tggggcagtc agagccgaaa gtgtcggctt cggcctgctt
     1321 gacccgattg accacgggcc gggcgatctt catggccttt tcgtaggttt cacttttgac
     1381 ggcgtaggtg ccgtcatgtc cggagcagcg ctcgatggcg gtgacctccg tgtcggggat
     1441 gaggctcagg aactcccgtg tcttcatgcc gatgttctgc acccgctggt gacaggccac
     1501 gtggtaggcg accttgccca ggggcttgcc gaacccggta tcgatcagcc cggccttgtg
     1561 gcgcagcacg aggtactcga aggggtcgaa gaaggccttt ttgacctgct cgacatcggc
     1621 gtcctcgggg aacatgagcg gcagctcctg tttgtacatc agcacgcacg aggggatcgg
     1681 cgcgatgatg tcgtaacctg cctcgatggc ctccaggaac agcggcaggt tcttatcctt
     1741 caggctcgcg accttgctca ggtcgccgag ctccagcttg ggcatgccgc agcatttggc
     1801 gtccttgaga atctttacgg gaatcccgtt gtgattgagc accgcgatga ggtcttcgac
     1861 cacctggggc tcgttgtggt cgccgtagca ggtgatgtag atggcgacct tgcccgtggt
     1921 tctctcgcag ggtttcggag tcagcgtcgc gccgatttct gtgtccagac gcttgttcag
     1981 cggtcgcggc gagaagttcg gcagcggtgc gtccgggtga atgccgaccg ccttgtcgcc
     2041 cagcttgcgc agcgcgctgt tgcccgcagc ggcgtttgcc atctgggcca cgcccggaat
     2101 gctgagcgtg tcgaagatgg ggtcggtgct ggtaatcagc ctgtcgcgcc acttcgtgtc
     2161 cttctcccgg aacttctgcg ctttcgcgcg gagcatgaga tgggggaagt ccaccgccca
     2221 ctcgtgaggc ggcaggtagg gacacttggt ctcggcgcac aggtcgcaca gaaagcactg
     2281 gtcgaccacc ttcatgtagt cggccttgtc gacgccgtcc acctccaggg tctcggactc
     2341 gtccaccaga tcgaacaggg tggggaacga gtcgcacagg ctcacgcagc gtcggcagcc
     2401 gtggcagatg tcgtagacgc gctcgagctc ggcgtaaagg gaggccttgt cgtagaactg
     2461 cgggttctgc caatccagcg gttgtcggtc tggtgcctcg aggctgcctt ccttcatagc
     2521 catcttcact gcctttgagt tgcggagact gggtcggtgt gaataagtcg ttgtgaataa
     2581 aaaggggccc tatcaggacc ccctccgttt ggttggcggt gaggtccgct cagcccatcg
     2641 cgtccagcgc tttctggaag cggttggcgt gagagcgttc agccttggcc agggtctcga
     2701 accagtccgc gatctcgtcg aagccctcgt cccgcgcggt cttcgccatg ccgggataca
     2761 tgtcggtgta ctcgtgggtc tcgccggcaa tggccgcctt caggttgttg ccggtggagc
     2821 cgatgggcag gcccgttgcc gggtcgccaa cggtctccat gtactcgaga tgtccgtggg
     2881 cgtgaccggt ttcaccctct gccgtagagc ggaaaacggc tgccacgtcg ttctcaccct
     2941 ccacgtcggc cttggccgca aagtagaggt agcggcggtt agcctgggat tcgccggcga
     3001 aggcgtcctt gagattctgc agagtgttgc tgtccttcag gtccatttct tcttgcctct
     3061 tactcgtttg tctttctggt cgaatgaacc cgatcgggtt cgcgtacggt gcagtatacg
     3121 aactggaatc ccgagatcca tttgaaagtt actatcggca caatagatag gaactatcaa
     3181 accgggctta ttgctagatt ctgcgaatgc agatgagaag cgttctcaaa accggtggga
     3241 ggtcaggtgg ccaggcgcca cgatcgggcg atgccgacga tgctggcgac ctgggtgaag
     3301 agggcgacgg caagcaccgc gcacgcgcct ggaattccca gccactcgaa ggcgatggca
     3361 ccggcgccgg cgatgctcag caaggcgatg ctgatctgta tggccagagc gctgacgggc
     3421 ccgcttttgc cgcgtcggcg cgagttgcgc gtgtcgagaa agagaatgaa gcccgccaga
     3481 agagaccc
//
LOCUS       DBNHOX010000762         3548 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1282448, whole genome shotgun sequence.
ACCESSION   DBNHOX010000762 DBNHOX010000000
VERSION     DBNHOX010000762.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1282448"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="AC16UZ_16550"
     CDS             <1..423
                     /locus_tag="AC16UZ_16550"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6248350.1"
                     /translation="LNSPYVVPGISDGGAHTHFSVQGAYPTDLLEWLVREEGVMTAER
                     AHYGLSRLPAHMCDLGDRGIIREGAPADLVIYNPETSRRTPSWDTLDKLRDLPANEWR
                     LVQRAEGLNYTLVNGVVTFEGLECTGATPGELMLHGER"
     gene            427..972
                     /locus_tag="AC16UZ_16555"
     CDS             427..972
                     /locus_tag="AC16UZ_16555"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6248351.1"
                     /translation="MEAEAGAGAQAIGAQAHEVARLREEIARLGARLRHLEAVQAVRE
                     TLLDYGHYHDRGLSAELAELFAEDAVLDISGFGDDLDVSISGRGAIRDMYASLDARSD
                     GPPPYKHAITNLRIEIDGEQALASSYLLDWGGPATDLGPGGSVYRQRLERQSDGRWLI
                     THKRIICTAELTVEAASSLPM"
     gene            1006..1974
                     /locus_tag="AC16UZ_16560"
     CDS             1006..1974
                     /locus_tag="AC16UZ_16560"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6248352.1"
                     /translation="MAGKLEGRVAVVTGAGRGVGRAVALLFAREGAKVVVADNGSNVD
                     GSGASTGPAHEVVEEIQAAGGIAMADTGDVSDWDQAEALINRPLEAWGALDILVNIAG
                     NFRVDNVTNVTRDNWDALRRVHMDGMMFTSRFAARHWAGGSGYGRLLNMTSDSAMSGV
                     PDTFAYAAAKSAVVGMTRAVANALVSYGVTANCLTQGSLTRMGDSYFGPDAEGRNRSE
                     LATPDQLPEQVAPLVTYLASPAAGHISGRIFGAYGFRYVRWSEPHHERALETEGAWDL
                     DDLFERFPETLGEGLSLERDLLWPMESLDAPSGETLDKGPALGETR"
     gene            2029..2913
                     /locus_tag="AC16UZ_16565"
     CDS             2029..2913
                     /locus_tag="AC16UZ_16565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6248353.1"
                     /translation="MKFGISMFPTDFSVGPAELARAIEHHGFESFWVSEHSHMPLTTQ
                     FPLADSVPRDYASMLDPFVALAAAAAVTSDILLGTAICLVPQRDPINCAKEVASLDHV
                     SGGRVVFGIGAGWNGPEMRNHGTDPSRRFRVMRERVEAMKALWTSEEAEYHGEMEDFD
                     PVWQWPKPVQRPHPPILVAGAHPNVLKRTVAYGDGWLPVVVPALNEQTRGRMTSLAEL
                     TEWVPRLRALADEAGKPPPIVTTLGMVPDRETLATYERLGIDRVVLGLTSAPMEDALK
                     QLEQHVVSVEAAGGRLGN"
     gene            3053..>3548
                     /locus_tag="AC16UZ_16570"
     CDS             3053..>3548
                     /locus_tag="AC16UZ_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248354.1"
                     /translation="MAGKRLLAALLFLWMTACSDDAQVVEEPAPSEEESSAPEDRPVV
                     QADLESVEVDAQPLLPSVLGAELSEARYDLEGLKQRGLLRVLVGYSHTHYFVDGLTIR
                     GISAENLRQFEPFLQKRLGSPRPRIHVLPIPVARDEMVAFLDQGFADMAVGNITITEE
                     RAELV"
BASE COUNT          616 a         1097 c         1221 g          614 t
ORIGIN      
        1 ctcaacagcc cctacgtcgt gcccggcatc tccgacggcg gtgcccatac ccacttttcg
       61 gtgcagggcg cttaccccac cgatctgctg gagtggctcg tgcgagagga gggcgtgatg
      121 accgcggagc gtgcgcacta cgggctgagc cgcctgcccg cgcacatgtg cgatctcggc
      181 gatcggggca tcattcgcga gggcgcgccc gcggatctgg tgatctacaa cccggaaacc
      241 agtcgccgca ccccttcctg ggacacgctc gacaagctgc gcgacctgcc ggcaaacgag
      301 tggcgcctgg tgcagcgcgc cgagggcctg aactacacgc tcgtgaacgg tgtggtcacc
      361 ttcgagggcc tggaatgcac gggcgcgacg cccggtgagc tgatgctgca cggggagcgc
      421 tgagacgtgg aggccgaggc cggcgccggg gctcaagcga ttggagcgca ggcgcacgaa
      481 gttgcccggc tgcgtgagga gatagccagg ctgggcgcgc ggctgcggca cctggaagcc
      541 gttcaggcgg tgcgagaaac gttgctggac tacggtcact atcacgatcg gggcctgtcc
      601 gccgagctgg cagagctttt tgccgaagat gccgtgctgg acatctccgg atttggcgat
      661 gatctggacg tctcgatttc cgggcgtggc gccattcgtg acatgtacgc gagcctggat
      721 gcgcgctcgg atggcccgcc gccctacaag catgcgataa ccaacctgcg catcgagatc
      781 gacggtgagc aggctttggc gtcgagctac ctactggatt ggggtggccc cgccaccgat
      841 ctcggaccgg gaggcagcgt ctaccgccag cgcctcgagc gacagagcga cgggcgctgg
      901 ctcatcacac acaaacgcat catctgcacc gcggagctca ccgtggaagc agcgtcgtcg
      961 ttgccaatgt agcgcagcaa tccgccacca gaacgggaga cttctatggc aggcaagctc
     1021 gagggcaggg tcgcggtggt gaccggtgcg ggacgcggcg tcggtcgcgc tgtcgcgctg
     1081 ctgttcgctc gcgaaggcgc gaaggtggtc gttgcggaca acggcagcaa cgtcgacggt
     1141 agcggcgcga gcacggggcc cgcgcatgag gtggttgagg agattcaggc ggcgggcggc
     1201 atcgccatgg ccgacaccgg ggacgtctcg gactgggatc aggccgaggc gctcatcaac
     1261 aggcctctcg aggcctgggg tgcgctggac atcctcgtga acatcgccgg caatttccgt
     1321 gtcgacaacg tcaccaacgt cacgcgcgat aactgggatg cgctgcgtag agtgcacatg
     1381 gacggcatga tgttcaccag ccggttcgct gcccggcatt gggccggcgg gagcggctac
     1441 ggccgcctgc tgaacatgac gtctgattcg gccatgtcgg gcgtgcccga tacctttgct
     1501 tacgccgccg ccaagtcagc ggtggtaggc atgacgcgcg cggtcgccaa cgcgctggtg
     1561 agctacgggg ttacggcaaa ctgcctgacc cagggttcgc tgacgcgcat gggagacagt
     1621 tacttcggtc ccgacgccga gggtcgcaat cgcagcgagc tggcgactcc ggatcagctt
     1681 cccgagcagg tcgctcccct tgtaacgtat cttgcttctc cagcagcagg ccacatcagc
     1741 ggtcggatat tcggtgccta cggcttcagg tacgttcgtt ggagcgagcc ccatcacgaa
     1801 cgggcattgg agaccgaagg cgcttgggat ctggacgatc ttttcgaacg ctttccggag
     1861 actctgggcg aaggattgag cctcgaacgc gatctgttgt ggcccatgga gtcgctcgac
     1921 gcgccttcgg gcgagaccct ggacaaaggt ccggcgctcg gtgagacccg ctgagtcgcc
     1981 gctaacattt ggtcagtcga aacatcaggc attgaggagg attcggcagt gaagttcggc
     2041 atcagcatgt tccccacgga tttcagcgtc gggccggcgg agctggcgcg ggccatagag
     2101 catcacggct tcgaatcgtt ctgggtctcc gagcactcgc acatgccgct gaccacccag
     2161 tttccgctgg ccgacagcgt gccgcgcgac tacgccagca tgctggaccc gttcgtggcg
     2221 ctggctgccg cggcggcggt caccagcgac atcctgctgg gcaccgcaat ctgcctcgtc
     2281 cctcagcggg atcccatcaa ctgcgccaag gaggttgcca gcctggatca cgtctccggc
     2341 ggacgcgtgg tgttcggcat cggtgccgga tggaacgggc cagagatgcg caaccacggc
     2401 accgatccgt ccaggcggtt tcgcgtgatg cgcgagcggg tggaagcgat gaaggcgctc
     2461 tggacgagcg aagaagccga gtaccatggc gagatggagg acttcgatcc ggtctggcag
     2521 tggccgaagc ccgtccagcg accgcatccg ccgatcctcg tcgcgggtgc gcatccgaac
     2581 gtgctcaaac gaacggttgc ctatggcgac ggctggcttc ccgtggtggt tccggcgctc
     2641 aacgagcaga cccgtggccg catgacgtcg ctcgccgagc tgacggaatg ggtccccaga
     2701 ctgcgcgcgc tcgccgacga agcgggcaag ccgccaccca tcgttacgac cctgggtatg
     2761 gtccccgatc gggagaccct tgccacctac gagcgcctgg ggatcgaccg ggtggtgctg
     2821 gggctcacct cggcgccgat ggaggacgct cttaagcagc tcgaacagca cgtcgtcagc
     2881 gtggaggcgg ctggtgggcg cctgggcaac tgatcgcccc gaggagggcg gccccgacga
     2941 ggttcggatc gttctttgat cacctccccc cggtggatat ccgtcagccg tcgggcaatc
     3001 attagcgggc attctgactt cgggtgcgac gactcaggtc cgggagggct cgatggctgg
     3061 gaagaggctg ctggccgcat tgctgtttct ctggatgacg gcttgcagcg atgacgcgca
     3121 ggtggtggag gagccggcgc cgtcagaaga ggaatcctcg gcacctgagg accgtccggt
     3181 cgttcaggcc gacctggagt ccgttgaggt cgacgcgcaa ccgctgttgc ccagcgtgct
     3241 gggcgcggag ttgagcgagg cccgctacga tctggaaggc ctgaaacagc gcggtcttct
     3301 ccgcgtgctg gtgggttact cgcataccca ctatttcgtg gatggcctga cgatccgcgg
     3361 tatcagtgcc gagaacctca ggcaattcga gccgtttctg cagaaacggc tcggatcacc
     3421 aagacccagg atccacgtgc tgccgatacc cgtcgctcga gatgaaatgg tcgccttcct
     3481 cgaccaggga ttcgccgaca tggcggtggg caacatcacg atcaccgaag aacgggctga
     3541 gctcgtgg
//
LOCUS       DBNHOX010000763         2243 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_447536, whole genome shotgun sequence.
ACCESSION   DBNHOX010000763 DBNHOX010000000
VERSION     DBNHOX010000763.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_447536"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="AC16UZ_16575"
     CDS             complement(<1..889)
                     /locus_tag="AC16UZ_16575"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6248355.1"
                     /translation="MHDSPPIERHRSLGQRDVVMYTVSAILLLETLTAAASIGAAAIF
                     WWLFLGVVFFLPFALICAEMGCTYPEQGGIYAWVRDAFGGRWGARASWGYWVNTAVWL
                     PAIYILFAGIFAQLYAPGLSLAGQIAIGIGLTWLTVGLNVVTLDVGKWVPNLGAAVKV
                     LVFLTLIGAASYHVATQGFANPINLETIRPTWSDSVAFIPAIIYGMLGFELVSASSEE
                     IKNPRRNVPRGILISGLIIISLYTLATTAMLAALPADEIDLVEGLVATLHQFFASSGA
                     GEIIVSILGAGALYTFFTNG"
     gene            complement(882..2231)
                     /locus_tag="AC16UZ_16580"
     CDS             complement(882..2231)
                     /locus_tag="AC16UZ_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HIR6248356.1"
                     /translation="MLEDANRTKHLHPFTDYAAYARSGGRVYTRGEGIFIYDAQGREL
                     LDGMSGLWCTSLGYSQPKIVDAVTEQLQRLPYYNSFFNCSNDAAVAMANALVEVTPPH
                     FNHVFFTNSGSEANDTNIRLVHRYFDLIGKPDKKIILSRENAYHGSTIAAASLGGMSS
                     MHKQFTRLPYVHHVQQPYWFELGSGRSPEEFGRQAAQAVADRIDELGADNVAAFIAEP
                     VQGAGGVIIPPETYWPAVQRILDERDVLLISDEVICGFGRTGRWFGCDYFGTRPELLT
                     FAKAVTNGYQPLGGVMVGDRIADVLTTQGGEFAHGFTYSGHPACCAAGLATLELYHEL
                     DTLSYVADDIAPYWASRWSELAAHPIVGESRTLGMLAAVELVSDKSGHTRLDSDGKAA
                     LFCRDEAIGGGLMVRAVQDSIISAAPLVSTREEIDLLIERLTQALNDTARHYGINDA"
BASE COUNT          401 a          693 c          763 g          386 t
ORIGIN      
        1 cgccgttggt gaagaacgtg tagagggccc cggcgcctag tatcgaaacg atgatttcgc
       61 ccgcaccgct ggatgcgaag aactggtgca gcgtggcgac gaggccctcc accaggtcga
      121 tctcgtcggc gggcaacgcg gccagcatcg ccgtggtcgc cagggtgtac agggaaatga
      181 tgatcagccc gctgatcagg atgccccggg gcacgttcct gcggggattc ttgatctcct
      241 cgctactggc gctgacgagc tcaaacccca acatcccgta gatgatcgcg gggatgaagg
      301 cgacggagtc gctccacgtc ggccggatgg tctctaggtt gatggggttc gcgaatccct
      361 gcgtggccac gtggtaactg gcggctccga tcagggtaag gaagacgagg accttgaccg
      421 ctgcccccag attcggcacc cacttgccca cgtccagggt cacgacgttc agcccgaccg
      481 tgagccaggt gaggccgatg ccgatggcga tctgcccggc cagggagagg ccgggagcgt
      541 agagctgcgc gaatatgccg gcgaacagga tgtagatggc gggcaaccac acggccgtgt
      601 tgacccagta gccccagctg gccctggccc cccagcgacc gccgaaggcg tcccgcaccc
      661 aggcatagat gccgccctgc tcgggatagg tgcagcccat ctcggcgcag atgagcgcaa
      721 agggcaggaa aaaaaccacg ccgaggaaca gccaccagaa gatggcggcc gcgccgatgg
      781 aagcggcagc ggtgagagtc tccaggagaa ggattgcaga gacggtgtac atgaccacgt
      841 cgcgctggcc cagcgatcgg tgcctttcga tcggcggcga gtcatgcatc gttgatgccg
      901 tagtgcctgg cggtgtcgtt caaagcctgg gtcagccgtt cgatcagcag atcgatctcc
      961 tcgcgggtgc tgacgagcgg cgcggcgctg atgatgctgt cctggacggc ccggaccatc
     1021 agaccgcccc cgatcgcctc gtcacggcag aaaagcgctg cctttccatc cgaatccagg
     1081 cgtgtgtggc cggacttgtc actgaccagc tctaccgctg ccagcatccc cagggtgcgg
     1141 gactctccga cgatgggatg cgcggcgagc tccgaccagc ggctcgccca gtagggggcg
     1201 atgtcgtcgg ctacgtagct gagcgtgtcg agctcgtggt aaagctccag ggtcgccagg
     1261 cccgccgcgc agcaggcggg gtggccggaa taggtgaatc cgtgggcgaa ctcgccgccc
     1321 tgggtggtca gcacgtcggc gatcctgtcg ccgaccatga cgccaccaag gggttgatag
     1381 ccgttggtca ctgccttggc gaaggtcaga agctccgggc gggtaccgaa gtagtcgcaa
     1441 ccgaaccagc gccctgtgcg gccgaaaccg cagatcacct cgtcgctgat cagcagaacg
     1501 tcgcgttcgt ccagaatgcg ctggacggct ggccagtaag tttccggagg aatgatgacc
     1561 ccgcccgccc cctgcaccgg ctcggcgatg aacgcggcca cgttatccgc gcccagctcg
     1621 tcgatcctgt ccgccacggc ctgggccgcc tgccggccga actcctccgg cgatcgtcct
     1681 gatcccagct cgaaccagta aggctgctgg acgtggtgca cgtagggcag acgagtgaac
     1741 tgcttgtgca tgctggacat gccgccgagg ctggcagccg cgatggtgct gccgtgatag
     1801 gcgttctccc ggctcaagat gattttcttg tcgggctttc cgatgaggtc gaagtagcgg
     1861 tgcacgagcc ggatgttggt gtcgttcgcc tcggagccgg agttcgtgaa gaacacgtgg
     1921 ttgaaatgcg ggggcgtcac ttccaccagg gcgttggcca tggcaaccgc cgcatcgttg
     1981 gagcagttga agaagctgtt gtagtaggga aggcgctgca gctgctcggt gacggcgtcg
     2041 acgatcttcg gctggctgta gccgaggctc gtgcaccaca gcccggacat cccgtccagg
     2101 agctcccggc cctgtgcatc gtagatgaag atcccctctc cccgggtgta gacacgcccg
     2161 ccggaacggg cgtaggccgc gtagtcggtg aaggggtgca ggtgcttggt gcggttcgcg
     2221 tcctcgagca cttcgctgct ttg
//
LOCUS       DBNHOX010000764         2302 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_513730, whole genome shotgun sequence.
ACCESSION   DBNHOX010000764 DBNHOX010000000
VERSION     DBNHOX010000764.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_513730"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="AC16UZ_16585"
     CDS             <1..455
                     /locus_tag="AC16UZ_16585"
                     /inference="COORDINATES: protein motif:HMM:NF014430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR6248357.1"
                     /translation="KRISERVELTPEAQLLKTVPGIGDKTAMLMWAEIGDITRFATSH
                     QLTSFAGLVPTTRSSGGRTTHGSLPRTGSPWLRWALVETTKTLKMKPGPVRAQFTRLA
                     RAKGNSKATVAAARKLCTYLYWMLKERVTYDEWLRRRSHAEVLEVRPR"
     gene            complement(605..1426)
                     /locus_tag="AC16UZ_16590"
     CDS             complement(605..1426)
                     /locus_tag="AC16UZ_16590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR6248358.1"
                     /translation="MIPSWVRRLAGSRLTPSQRRNAEYDRDTVRIIKTLPPNATCIDV
                     GAAAGSVLRAMVRAVPDGRHFAFEPRPDAAQGLRRSFPGVEIQEVALSDRAGAAPFQL
                     VVDSPMWSGLRVQTYPQPDARINQITVRTDRLDDVLSADLRVDFIKIDVEGAEALVLR
                     GAERTCRRWAPLIVVEYGLAAREHYGVSPTDFFGLISGLGLDISLLQDWLRGRPPLDI
                     RGFDQQCQSHWYFIAHPGRSDPATGTPVSDNASSERTEVKGRKSTTPASERSEAG"
     gene            complement(1687..1863)
                     /locus_tag="AC16UZ_16595"
     CDS             complement(1687..1863)
                     /locus_tag="AC16UZ_16595"
                     /inference="COORDINATES: protein motif:HMM:NF019540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HIR6248359.1"
                     /translation="MAALKRIGWEIKRERGSHKVLAREGWSDFVFAFHDSDEVGPRML
                     ARIAKHTGLTPDDL"
     gene            complement(1896..2102)
                     /locus_tag="AC16UZ_16600"
     CDS             complement(1896..2102)
                     /locus_tag="AC16UZ_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR6248360.1"
                     /translation="MKLLIEVEREDDGRWIAEVPSLPGVLAYGATELEARAKAQALAL
                     RVLADRLEHDEANPDLMNISFSAA"
BASE COUNT          424 a          746 c          737 g          395 t
ORIGIN      
        1 cgaagcgcat cagcgaacgc gtcgagctca cgcccgaggc tcagctgctc aagaccgtgc
       61 cgggcatcgg cgacaagacg gccatgctga tgtgggccga gatcggagac atcacacgct
      121 tcgcgacctc acatcagctc acgagcttcg ccgggctcgt gccgacgacc cgaagctcgg
      181 gcggtcgcac gacacacggc tcgctgcccc gtacgggcag tccctggctg cggtgggcgc
      241 tcgtcgagac gacgaagacg ctcaagatga agcccggacc ggtgcgcgct cagttcacgc
      301 gactcgctcg agcgaagggc aactccaagg ccacggtcgc cgcggcccgg aagctgtgca
      361 cctacctgta ctggatgctc aaggagcgag tgacgtacga cgagtggctg agaaggcgga
      421 gccacgccga agtgctggag gtgcgcccga gataacgcct ggcgccctac atacgggccg
      481 ccgtcaattc gaatgggcca cctccttcga atccaggatc ctgtgcccca cagcgcacga
      541 agaggcggtt gaagactcct tctcgccacg atcctacgaa gccctcggcc ccggcgtccc
      601 ggttctatcc agcctccgag cgctcgctcg caggcgtcgt actcttgcgc cccttgactt
      661 cggttctttc agaggaggcg ttatcagaca ctggagttcc ggtggctgga tccgacctcc
      721 caggatgggc aatgaagtac cagtgggact ggcattgctg gtcgaagccc cggatgtcca
      781 aggggggccg gccgcgaagc cagtcctgaa gcagtgaaat gtcgagtccc agacccgaga
      841 taaggccgaa gaagtcggtg ggcgagactc cataatgctc tctggcggca agaccgtact
      901 cgacgacgat gaggggagcc catcgacggc aggtgcgctc ggcgcctcgc agcacaaggg
      961 cctcggcgcc ttcgacatcg atcttgatga agtccacacg gaggtcggcg gagagcacgt
     1021 cgtcgaggcg gtctgtgcgg acggtgatct ggttgatccg cgcatccggc tgcggatagg
     1081 tctgcaccct aagtccgctc cacatcggtg agtcgactac tagttggaat ggagcggctc
     1141 cagctcggtc gctgagcgcc acttcttgga tctccactcc tgggaaggaa cgacgaaggc
     1201 cctgggccgc atcgggccgt ggctcgaacg cgaagtgacg accgtccggg accgccctga
     1261 ccatggctcg aaggacagag ccagccgccg cgcctacgtc gatgcacgta gcgttgggcg
     1321 gcaacgtctt gatgatccgg acggtgtctc gatcgtactc cgcattccta cgctgagacg
     1381 gtgtgagtcg agacccggca agacgccgaa cccaggacgg gatcatcctt gatgtgagct
     1441 ggggattggg cgctgaggcg ctaactctcc tacacctgcc accgacgcgt ccagtgtctg
     1501 ataacgttca gagagtgccg acgcggcgcc attggttgcc cgcaggccta ccagccgagc
     1561 cgcgtacagc ggcgacggtt tgtagcaggg cgccgtgtac tccggagcgc gcgtagcgcg
     1621 cggaggcggc actcgcattg ttagcggacg cgccacccga taagactttc gggcacagct
     1681 ctgctgctac agatcgtccg gggttaggcc ggtatgtttc gcgattctgg ccagcatccg
     1741 gggacccact tcgtccgagt cgtgaaaggc gaagacgaaa tcggaccagc cctcgcgcgc
     1801 cagcactttg tgagaccccc gctcgcgctt gatctcccac ccgatgcgct tcaaggcggc
     1861 caatactcgc ctcgcgcgag tactcggcca tgagctcacg cggccgagaa ggagatgttc
     1921 atgaggtctg gattggcctc atcgtgctct agccgatcgg ccagtactct tagagcgagc
     1981 gcctgggcct tcgcgcgcgc ctccagctcc gtggctccgt aggctagaac gcccggtaga
     2041 gacgggactt cggcgatcca acggccgtca tcttctcttt cgacctcaat caggagcttc
     2101 atgccacacc tcgggatcgg tctgatgacc tcaatctacg cctgaccgga gccgcccggt
     2161 actcgactcc accagctact ccaggccgcg ggaggccgat ccacactgtc ttcggcgtgt
     2221 ccgctaacgt tcagcggttg ccggcgcggc gcctccgggg gcgccgtgct tcagcgaagt
     2281 gagcgtcagc gaacgcagcc gg
//
LOCUS       DBNHOX010000765         3122 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1090354, whole genome shotgun sequence.
ACCESSION   DBNHOX010000765 DBNHOX010000000
VERSION     DBNHOX010000765.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1090354"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..795
                     /locus_tag="AC16UZ_16605"
     CDS             25..795
                     /locus_tag="AC16UZ_16605"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR6248361.1"
                     /translation="MRLVSWNVNGLRACAKKGFLGFLETSGADFVALQEVRAFPDQLD
                     DGLRAPPGWHVCFSPAERPGYSGVALYARAAPDRVQTSLGKPDFDREGRLLSAHFGRL
                     AIVSVYFPKGSGKDRDNSRVGYKLDFYAAVFDHVEQLRRRGPVFVVGDYNTAHEPIDL
                     ARPKTNTKSSGFLPEERAEITRWIDAGWVDVFRRQHPGQPDHYTWWRQWGNARRDNVG
                     WRIDYVLASPSAARRVRQAFIWPEVTGSDHCPVGVDFE"
     gene            999..1304
                     /locus_tag="AC16UZ_16610"
     CDS             999..1304
                     /locus_tag="AC16UZ_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248362.1"
                     /translation="MCRHYDESASAGRCREERADPPPEKDVANFCDWFQPGKNAFQEG
                     RTTAGENARSRLDALFGDSQGPAEEPDEAPPKPEDGPRSAEEKARAELDRLFSRDAD"
     gene            1666..>3122
                     /locus_tag="AC16UZ_16615"
     CDS             1666..>3122
                     /locus_tag="AC16UZ_16615"
                     /inference="COORDINATES: protein motif:HMM:NF006624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HIR6248363.1"
                     /translation="MSLVDALEYAAQGDTGLNFYDGSGSLSAALPYAQLAQDARILAT
                     RLLGLGVKRGARVGIVAETDPMFPRFFFACQYAGLIPVALPAGLQMGGGEAYVEQLSR
                     MLESCGAAVAVAPESHLSFLKRATRQLSLRMSGSPEDFDALPAVPVKLLPLSADEPAY
                     LQYTSGSTRFPRGVEMTQTAVLANLTEIAEIGTRITADDRIVSWLPYYHDMGLVGCLL
                     VPVATQISADFLSPRTFAMRPRLWLKLLSENRGTISSSPPFGYELCATRVRMTDTERY
                     DLSAWRVACVGAERINPRPLKQFANVLAPAGFNPKAFLPCYGMAEVGLAISFAPLEDG
                     YFVDDVAKASMSAEGLAIEATEGDRAKGNTLSFVDCGAALPSYDVSIRDESGRELDER
                     ECGRIWVRGPSLMRGYFRDPVTTAEVMKPGGWLDTGDIGYRIGRRLFLTSRAKDVIII
                     NGRNIWPQDLEFLAEQQVEVRPGDSSAISVPGEDGA"
BASE COUNT          559 a         1018 c          989 g          556 t
ORIGIN      
        1 tcaaacagga gcgagactgg atcgatgcga cttgtcagct ggaacgtcaa cggcctgcgc
       61 gcctgtgcga aaaagggctt tctcggattt ctcgagacct ccggggccga cttcgtcgcc
      121 ctgcaggaag tgcgcgcgtt ccccgatcag ctggacgacg gcctgcgagc cccgcccggc
      181 tggcacgttt gtttctctcc cgcggagcgc cccggctaca gcggcgtcgc cctctacgcc
      241 cgggccgccc ctgatagggt tcagaccagc ctcgggaagc cggacttcga tcgggagggg
      301 cggctgctca gcgcgcattt cggccgcctg gccatcgtga gcgtctactt ccccaaaggc
      361 agcggcaagg atcgggacaa cagccgcgtc ggctacaagc tcgattttta cgccgccgtg
      421 ttcgatcacg ttgagcagct gcgccggaga ggtcccgtat ttgtggttgg cgactacaac
      481 acggcccacg agcctatcga cctcgcccgt ccaaaaacca acaccaagtc gagcgggttt
      541 ctgcccgagg agcgagccga gatcacgcgc tggatcgacg ccggctgggt ggacgtcttc
      601 cgccgccagc atccggggca gcccgaccac tacacgtggt ggcggcagtg gggcaacgcc
      661 cgccgggata acgtcggctg gcgaatcgac tacgtgctgg cctcgccatc ggctgcaagg
      721 cgggtcaggc aggcgttcat ctggcccgag gtgaccggat ccgatcactg cccggttggc
      781 gttgacttcg aatgagacga cgccaccggg cccgcattga cccgcattgc ctcgagcgca
      841 tcggatttct atagtccacc catcgtccag gccagaggca gtctcccgtg gcagacgcac
      901 gttgctggaa ttgtggtacg tcgatcgacg acgtgcccag acccataagc cggcaagcaa
      961 cctgttctaa gtgcttccac gaactgcact gttgtcgcat gtgccggcac tacgatgagt
     1021 ccgccagcgc gggaaggtgc cgggaggaac gtgcagatcc ccctcccgaa aaggacgttg
     1081 ccaacttttg tgactggttt cagccaggca agaacgcctt ccaggaaggc cgcaccacgg
     1141 cgggcgagaa tgcccgctca cgcctcgatg cgctgttcgg cgattcgcag ggcccggcgg
     1201 aagagcctga cgaggcgccg ccaaaacccg aggatgggcc ccgaagcgcc gaggagaagg
     1261 cccgcgccga gctcgatcgc ctgttttcgc gggacgctga ctgattgcgc cccgcgtcgg
     1321 atatcgctcc gaggcgagat gggcccgacc agcgacatcg caaatctgtg acattcacac
     1381 gccatttccg tgaaacgcac ccgttatcgc gggtctacaa tccgtaggtc tctgatttcg
     1441 tttgaaaaga cactatcgct ctcagacgaa tttgcaggta gactgcgcca ccagaaatat
     1501 aagtgccgga tgattcgccg gccgatggag agcctcggtt tgttgggcag ctgtgttcgg
     1561 aggggttcga gcacaacgaa aagagcaaac acgacaattt gagctggtta tcacatgagc
     1621 cctaccactc gttgtagcgg gatatcgcac cgcacacgtg gctttatgag cctggttgat
     1681 gccctggagt acgccgcgca aggcgacacg gggctgaact tctatgatgg ctccgggagc
     1741 ctcagcgccg cgttgccgta cgcgcagctg gcgcaggacg ctcggatcct ggcgacccgg
     1801 ctgctcggcc tgggcgtcaa acgcggcgcc cgggttggca tcgttgccga aaccgacccc
     1861 atgttcccgc gattcttctt tgcctgtcag tacgccgggt tgatcccggt ggctctgcct
     1921 gccggtctgc agatgggcgg cggcgaggcc tacgtggagc agctcagtcg catgctggaa
     1981 agctgcggag ctgcggtggc ggtcgcgccg gaatctcacc tcagcttcct gaagcgtgcg
     2041 acgcgacagc tgtcgctgcg catgtccgga tcgccagagg acttcgacgc cctgccagcg
     2101 gtgccggtga agctgctccc cttgagtgcc gacgaacccg cctacctgca gtacacgtcg
     2161 ggaagcaccc gcttcccccg cggcgtcgag atgacccaga cggccgtcct cgcaaacctg
     2221 accgaaatcg ccgagatagg cacccgcatc accgccgacg accggatcgt ttcatggctg
     2281 ccctactacc atgatatggg cctggtgggt tgcctgctgg taccggtggc aacgcagatt
     2341 tcagccgact tcctgtcgcc gcgcacgttt gccatgcgtc cacggctttg gctgaagctg
     2401 ctctcggaaa atcgcgggac gatctcgtcc agcccgccgt tcggctacga gctgtgcgcg
     2461 acccgcgtcc gcatgacgga cacggagcga tacgacctca gcgcctggcg cgtcgcctgc
     2521 gtcggcgcgg agcgcatcaa cccgcgtccg ctgaagcagt tcgccaacgt gctggctcct
     2581 gccggcttca accccaaggc gtttctgccc tgctacggca tggcggaggt gggtctggca
     2641 atcagcttcg ccccgctgga ggacggctac ttcgtcgacg acgtggccaa ggcctcaatg
     2701 tctgcggaag gcctggccat cgaggccacc gagggcgacc gtgcgaaggg caacaccctc
     2761 tcgttcgtcg actgcggcgc ggcgcttcca agctacgacg tctccatccg cgacgagtcg
     2821 ggccgagagc tcgacgagcg cgagtgcgga cgcatctggg ttcgggggcc aagcctgatg
     2881 aggggctact tccgcgatcc cgtgaccacc gccgaggtca tgaagcccgg cggatggctc
     2941 gataccggcg acatcggcta ccgcatcggc aggcgtctgt tccttacctc gcgggccaag
     3001 gacgtgatca tcatcaatgg ccgcaacatc tggccgcagg atcttgaatt cctcgctgaa
     3061 cagcaagtgg aggtcaggcc tggtgactcg tcggccatct ccgtcccggg cgaagacggc
     3121 gc
//
LOCUS       DBNHOX010000766         5303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_255542, whole genome shotgun sequence.
ACCESSION   DBNHOX010000766 DBNHOX010000000
VERSION     DBNHOX010000766.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_255542"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="AC16UZ_16620"
     CDS             <1..265
                     /locus_tag="AC16UZ_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248364.1"
                     /translation="RISTVDRPGWCLSPGRVPEPGDHIYCLEGPAEVVRLLGRTSDGS
                     RLLELRCEGRPKPFFSSSSNILFRSEGEAPSAFLDPSQAVGGC"
     gene            474..1580
                     /locus_tag="AC16UZ_16625"
     CDS             474..1580
                     /locus_tag="AC16UZ_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248365.1"
                     /translation="MKPTRLMRTIRYSRSWKHGSPDVERTGVTIQREGKEIPATFVRP
                     RAATRRLPAWIAIGGVSLMGRFHPQLVRFTEALASTGACVLVPEFPEWRRLSVNPRIT
                     LPTIRASVDHLNGRADVISECYGVIGFSFGGAGAVLAASDDEVVDHIGGAVVFGGYSC
                     LRRTLACMLTGEHDWRERRHQLQPDPYGRWVVASNYLTRVPGYEDATDVAEAMNRLAA
                     EASGQRISAWEPFHDSMITGLRHSIPPKRRPLFDMLATPTHEERPHSDECSEFAQALA
                     GICAAAEPQLDPIRMLHRTRVPTQVIHGRGDRLVPFTEGMRLMEGIPRTCRKGATVTG
                     LFNHSKDHVPPGPVERAYETSLLLRALHRLVNTV"
     gene            1637..2239
                     /locus_tag="AC16UZ_16630"
     CDS             1637..2239
                     /locus_tag="AC16UZ_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248366.1"
                     /translation="MGTSLEQRLDALCEEGRDFWHDFDARVRQETWHPFVAADYDLVR
                     ESLLTLRRPGRRFLEWGSASGVITIMADLMGFDACGIEIDVSLVGAARELAKRTRSPA
                     RFATGSFIPMGWEWNPPDGDRRTATIGQDRSGYVELGHALEDFDVVYGYPWSGEEPLM
                     RDLMRSHGSSEAILMLHCVEHGVRLYRQGVEVDAATLGVP"
     gene            complement(2236..3510)
                     /locus_tag="AC16UZ_16635"
     CDS             complement(2236..3510)
                     /locus_tag="AC16UZ_16635"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6248367.1"
                     /translation="MAFATLKLHETLLEGVDSLGFACPTPIQSEAIPHALEGRDLLAC
                     AATGSGKTAAFLLPVLHRIIARPRGATRALVLAPTRELALQIVAETKALARFTDLTAA
                     PVHGGVKMGPQEKAFRRGVDVIVATPGRLLDHFQHGYASLTGLEFLILDEADRMLDMG
                     FLPDIRRILRHIPQPKQTLFFSATMPPEIERLTREILDRPAKVALQRKAAPAAGVTQV
                     AYPVAASLKSSLLVELFRSGQMKEALVFTRTKHRANRLAKHLNRKGITAERIHGNRSQ
                     SQRTKALAGFKRGDFRVLVATDIAARGIDVEALGHVVNFDVPNVAEDYIHRVGRTARA
                     QMTGEAFTLVSPEERHDFGKIERAVGAKIERAQLEGFAYEATAEATGPATNGQHVRGS
                     GRSRGTSRKAGGEEGPEKPSRPRRRRYGKRRG"
     gene            3995..4984
                     /locus_tag="AC16UZ_16640"
     CDS             3995..4984
                     /locus_tag="AC16UZ_16640"
                     /inference="COORDINATES: protein motif:HMM:NF024852.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HIR6248368.1"
                     /translation="MISSATRGTLLLTGASGFVGRHAYGALVDAGWHVRCATRDVGRA
                     HRRWPERDWVSLDVTDEASVVRALEGCAAALYMIHGMASHEGDFREAEVRQAEVFATA
                     AAAAGLDRIVYLGGVAAPSDASDHLRSREDVGEVLRAGSVPTVELRASMIVGHGSLSW
                     LIVRDLAARLPIMILPRWLESRTEPVAIDDVVTAVVRALDLEISGSEWFDVPGPEILS
                     GREILERTADALGVRRAFMIQVPFLSPGLSSHWVRFVTRADWSVAREIVVGLKTDLVA
                     RDDRFWTLIDHGERLSFDEAARRTIEEELAGPPIRGLWGLIERMRVRIARTLG"
     gene            5110..>5303
                     /locus_tag="AC16UZ_16645"
     CDS             5110..>5303
                     /locus_tag="AC16UZ_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248369.1"
                     /translation="MGTTAVVLGVAALTFEGRAVLGPGLHSRMWLIGLPAGLLMAGGT
                     VLLFGPITSAFPGLTEDVERL"
BASE COUNT          847 a         1744 c         1772 g          940 t
ORIGIN      
        1 ccgcatctcc acggtcgacc gcccgggctg gtgtctctcg cccggtcgcg tacccgagcc
       61 aggagaccac atctactgcc tcgagggacc ggcagaagtc gtgcgcctcc tgggacgcac
      121 cagtgacggc agccgtctcc tcgagctccg ttgcgagggt aggccaaagc cgttcttctc
      181 gtcgtcctcg aacatcctct tccggtcgga aggcgaggct ccctcggcct tcctggatcc
      241 ctcccaggcc gtcggaggct gctgatgctg aggggcgtgt cgcgaacagc cctgtatgcc
      301 ggcagcttcc gccaggccaa gatgctgcga cgtgccgcgg ctggcagagg tgtgagggcg
      361 gagatctcgt tgactcggga tcgcaggctt tgtcgcttct ggaccggctt catgccccac
      421 gggcagttgc accgccgctt cggccgcgca ggctatcgtt atgcgggagg gccatgaaac
      481 cgacacgcct gatgcggacg atccgctatt ccaggagctg gaaacacgga tcgccggatg
      541 tcgagcgcac cggggtgacc atccagcgtg aggggaaaga gatacctgcc acgttcgtgc
      601 gacccagggc tgccacccga cggctccccg cctggatcgc catcggaggg gtctcgctga
      661 tgggtcgctt ccacccccag ctcgtgcgct tcacggaagc gctcgcttcg acgggggcct
      721 gcgtgctcgt accggagttc cccgagtggc gccggctcag cgtgaacccc cgcatcactc
      781 tgccgaccat ccgcgcatcc gtcgaccacc tgaacggccg ggcggatgtg atttctgaat
      841 gctacggcgt gatcggcttc tcgttcggcg gagcgggggc ggtactcgcc gcttccgacg
      901 acgaggtcgt cgaccacatc ggcggcgccg tagtcttcgg gggatattcc tgcctgcggc
      961 gcaccttggc ctgcatgctt accggtgagc acgattggcg cgagcgccgt caccaacttc
     1021 aacctgatcc ctatggccga tgggtcgtcg ccagcaacta tctcacccgt gtaccgggct
     1081 acgaggatgc cacggacgtg gccgaagcca tgaaccggct cgccgcggaa gccagcggtc
     1141 agaggatctc ggcttgggag ccgttccacg attcgatgat cacggggctt cgccattcca
     1201 tcccgccgaa gcggcgcccc ctgttcgaca tgctggcgac cccgactcat gaggaacgcc
     1261 cccactccga tgagtgctcc gaattcgcgc aggcgctcgc aggcatctgc gccgccgccg
     1321 agccgcaact cgatccgatc cggatgctcc atcgcacgcg ggtgcccacg caggtcatcc
     1381 atgggcgcgg ggaccgcctc gtgccgttca cggagggcat gcgcctcatg gaggggattc
     1441 cgagaacgtg tcggaagggg gcgaccgtga cgggattgtt caaccacagc aaggatcacg
     1501 tgccgccggg ccccgtcgaa cgcgcctacg agacaagcct gctcctccgc gcactccacc
     1561 gactcgtcaa caccgtctaa tcccgaagcc catgcttccc gaaaacacat cgtgcgtcca
     1621 gcgcaaatca gtcgagttgg gcacatcgct ggagcagcgc ctcgatgcgc tctgcgaaga
     1681 gggccgtgac ttctggcacg acttcgacgc ccgggtccga caagagacct ggcacccctt
     1741 cgtcgccgcc gactacgacc tggtgcgcga gtcgctcctc acgcttcgcc gacccggccg
     1801 tcgttttctg gagtgggggt cagccagcgg cgtcatcacc atcatggcgg atctgatggg
     1861 cttcgacgcc tgtggcatcg agatcgacgt ctccctcgtt ggagcggcac gagagcttgc
     1921 gaaacgcaca cgttcgccgg cgcgtttcgc caccggcagc ttcattccta tgggatggga
     1981 gtggaaccca ccggacggtg acagacgtac ggcgacgatt ggccaagaca ggtccggcta
     2041 tgtcgagctg gggcacgccc tggaggactt cgacgtcgtc tacggatacc cgtggtcggg
     2101 cgaggaaccc ttgatgcgag acctgatgcg ctctcacggc agcagcgaag cgatactgat
     2161 gctgcactgc gtagagcacg gtgtccggct ctaccggcag ggtgtagagg tcgacgccgc
     2221 aacgttggga gtgccctagc ctctccgctt tccgtaccgt ctccgcctcg ggcgcgacgg
     2281 cttctctggc ccttcctccc ctcccgcctt ccgtgacgtg cccctcgatc ggccggaccc
     2341 acgcacatgc tgaccgttcg tggccgggcc ggtagcctcg gccgtggctt cataggcgaa
     2401 gccctccagc tgcgctcgct cgatcttcgc acccaccgct cgctcgatct tgccgaagtc
     2461 gtgcctctct tcaggtgaca ccagcgtgaa ggcctccccg gtcatctgag cgcgagcagt
     2521 gcggccaacg cgatggatgt agtcctcggc cacgttcggc acatcgaaat tcacgacgtg
     2581 cccgagtgcc tccacgtcga ttccccgcgc cgcgatatcc gtcgcgacga gcacgcgaaa
     2641 gtcgccccgc ttgaagcctg cgagtgcctt cgtgcgctgg gattgggacc gattgccgtg
     2701 aatccgctcg gcagtgatgc ccttccggtt cagatgcttg gccaagcgat tggcccggtg
     2761 cttcgtgcgc gtaaacacga gagcttcttt catctggcca ctgcggaaga gctcgacgag
     2821 cagcgagctc ttgaggctgg ccgcgacggg atacgcgacc tgagtcactc ccgcggcagg
     2881 cgccgccttg cgctgcaagg caacctttgc cggacgatcc agaatttcgc gcgtaagcct
     2941 ttcgatctcg gggggcatcg tcgcactgaa gaagagcgtc tgcttcggct gcggaatgtg
     3001 ccgcaggata cgccgaatat ccggcaggaa gcccatgtcc agcatgcgat cggcttcgtc
     3061 gagaatcagg aattcgagcc cggtcaggct ggcgtagccg tgttgaaagt gatcgagtag
     3121 gcgccccggc gtcgccacga tcacgtcgac accacggcgg aacgccttct cctggggccc
     3181 catcttcacc ccgccgtgca cgggggctgc ggtcaggtcc gtgaaccggg ccagggcctt
     3241 cgtctcggcc acgatctgga gtgccagctc acgggtcggg gcgagaacga gcgcgcgcgt
     3301 cgcgccccgc gggcgggcga tgatgcggtg aagcactggg agcaggaagg ccgccgtctt
     3361 gccgctcccg gtcgcggcac atgccaggag gtctcgacct tcgagggcgt gcgggatggc
     3421 ttcggattga atgggtgtgg gacaggcaaa gcccagcgag tcgacccctt cgagaagggt
     3481 ctcgtgcaac ttcagtgtgg cgaacgccaa tgtgtagtcc agttgggagg cgccctgcag
     3541 gccggccgtc caaacgttgg cggctcgagt aggcgggcgc agaatcgaga gtgagcgccg
     3601 acccccggaa ccgggggctc gaggcgaacc tcactcggag aagataaccg aaagttgctc
     3661 tgcgagaacc tcgtccggtg aggccggcgg cgtggcgacg cgcagaaggt ccccgatcag
     3721 cgaacgcagg agtctcgatg gacctgggta tgaacttcca cgtcgtctgc aggcgccggt
     3781 cgacggtcac ccgccggaag aacgccaagg cccacaggac gttggggcgc caaagccggc
     3841 ctttcctctg ggtctcgcca acaacgtgat cgctcgcctt tcgagccggg ccgtgacaat
     3901 gtcagagggt gccctccgcg gggccccagc cactcgagct gggcagaccg cggtctcccg
     3961 cgttggacgg accttcccga actctgagcc gttgatgatt tcatcagcca cacgagggac
     4021 cctgttgctt accggcgcca gcggcttcgt gggacgacac gcctatggag cgctcgtgga
     4081 tgccggctgg catgtacggt gcgccactcg cgacgtgggc cgggcccatc ggcgctggcc
     4141 ggagcgcgat tgggtgagcc tggacgttac cgacgaggcg tcggtggtgc gtgccctcga
     4201 ggggtgcgca gcggccctgt acatgatcca tggcatggcc tcgcacgagg gagacttcag
     4261 agaggccgag gtccggcagg cggaggtgtt cgccacggcg gccgccgccg cgggactcga
     4321 tcgcatcgtg tacctggggg gcgtggcagc gcccagcgac gcgtccgacc atctgcgaag
     4381 ccgcgaggac gtaggggagg tactgcgagc cggctcggtc cccacggtgg agctgagagc
     4441 cagcatgatc gtaggccacg gaagcttgag ctggctcatc gtccgggatc tcgccgcgcg
     4501 actccccatc atgatcctgc cgaggtggct cgagtcgcgc acggagccgg tcgcgatcga
     4561 cgacgtcgtc accgccgtcg ttcgcgctct cgatctcgag atttcgggca gcgagtggtt
     4621 cgacgtgccg ggacccgaaa tcttgtccgg tcgcgagatc ctggagcgca ccgccgacgc
     4681 cctcggggtg cggcgggcct tcatgatcca ggttccgttc ctctctcccg ggctctcatc
     4741 acactgggta cggttcgtca cgagagccga ttggtccgtc gcccgcgaga tcgtcgtggg
     4801 gctcaaaaca gatttggttg cccgagacga tcgcttctgg accctgatcg atcacgggga
     4861 acgactgagc ttcgacgagg ccgcccggcg gacgatcgaa gaagagctgg caggcccgcc
     4921 gattcgggga ctctggggcc tgatcgagcg catgcgggtt cgaatcgccc ggactctcgg
     4981 ttgagcggtc gagtgggcgg ggtcgatcgg ggcccgagcc tgacgtccgc agaatggcgg
     5041 ggcgtcgcgt actgcctcct gtgggcttca ggtgcgtggc tctcggggca tctcgggatc
     5101 tggcctgcga tgggtacgac tgcggtcgtg ctcggtgtgg ctgctctgac cttcgaaggc
     5161 cgggctgtcc tgggccctgg gcttcactcg cgcatgtggc tgatcggtct gccggccggt
     5221 cttctgatgg cgggcggcac ggtcctgctc ttcggaccga tcacgtccgc ctttccaggt
     5281 ctcacggagg atgtcgagcg gct
//
LOCUS       DBNHOX010000767         1971 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1604697, whole genome shotgun sequence.
ACCESSION   DBNHOX010000767 DBNHOX010000000
VERSION     DBNHOX010000767.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1604697"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /gene="gltD"
                     /locus_tag="AC16UZ_16650"
     CDS             <1..202
                     /gene="gltD"
                     /locus_tag="AC16UZ_16650"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351983.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HIR6248370.1"
                     /translation="SDSLGIAYDDRSNYSAEYGRYATSVDKVFAAGDCRQGQSLVVRA
                     INEGREAAREIDRFLMGASQLP"
     gene            complement(210..1295)
                     /gene="aroB"
                     /locus_tag="AC16UZ_16655"
     CDS             complement(210..1295)
                     /gene="aroB"
                     /locus_tag="AC16UZ_16655"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020302367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR6248371.1"
                     /translation="MTLEVDLGDRSYPIHIGDGLLGRGELLRDQVHGSQVAVITNDVV
                     GPLYLPRVAQALAGIDADVYTLPDGEQHKNLESYGAIMDFLMERRHNRSTTLVALGGG
                     VVGDITGFVAATFQRGVAFVQVPTTLLAQVDSSVGGKTAINHPRGKNMIGAFYQPRCV
                     IADTTVLNSLGDRELRAGLAEVLKYGIIADAGFFDWLESNANALLSREPETLAVAIRR
                     SCEIKAAVVAEDERESGIRAILNYGHTFGHAIETLTGYGELLHGEAVAAGMVLAADLS
                     ARQGWLTGTEAKRIKAALEAFGLPVVAPSIEPDDMLSAMSIDKKVVNGRLRLVLARAI
                     GEAAVTEDVDEPALRATLAAGEALCDG"
     gene            complement(1301..1825)
                     /gene="aroK"
                     /locus_tag="AC16UZ_16660"
     CDS             complement(1301..1825)
                     /gene="aroK"
                     /locus_tag="AC16UZ_16660"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010955625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HIR6248372.1"
                     /translation="MRTQNLFLVGLMAVGKSTVGRCLARDLDMAFYDTDEVLEERAGA
                     EVAWIFDVEGESGFRDREQQVIEELTAKSGVVLATGGGAVLRAANRQNLSARGIVVHL
                     DSPLERLVERTARDNKRPLLQGGDARETLARLQRERAPLYAEIADYTFVTDRQGPKVL
                     ARTIERKLREDGLI"
BASE COUNT          342 a          695 c          589 g          345 t
ORIGIN      
        1 ctccgattcc ctcggaatcg cctacgacga ccgctccaac tactcagccg agtacggcag
       61 gtacgccacc agcgtcgaca aggtgttcgc cgccggcgac tgccgacagg gtcagtccct
      121 ggtggtgcga gccatcaacg agggacgcga ggcagcgcgc gagatcgacc gcttcctcat
      181 gggcgcctca cagctgccct gacgggcggt cagccgtcac acagagcttc gcccgccgcc
      241 agcgtggccc tcagcgcagg ctcgtcgacg tcttcggtga cggccgcttc cccgatggcg
      301 cgcgcgagca ccagtcgcaa gcggccattc acgaccttct tgtcgatgga catcgcgctc
      361 agcatgtcgt ccggctcgat ggaaggtgcc accacgggca atccgaacgc ctcgagcgca
      421 gctttgatgc gcttggcctc cgtaccagtc agccagccct gccgagccga aagatcggcc
      481 gccagcacca tgcccgccgc caccgcctca ccatgcagga gctcgccgta acccgtcagc
      541 gtctcgatgg cgtgaccgaa agtgtggccg tagttcagga tcgcccggat gccggactcc
      601 cgttcgtcct cggccaccac ggccgctttg atctcgcagg accggcgtat cgccacggca
      661 agcgtttccg gctccctgct gagcagcgcg ttggcgttgc tttcgagcca atcgaagaac
      721 ccggcgtccg caatgatgcc gtacttgagc acctccgcga gcccggcacg aagttcccgg
      781 tctcccagcg agttcagaac cgtggtgtcc gcgatgacgc agcgtggctg gtagaacgcc
      841 ccgatcatgt tctttccgcg cggatggttg atcgccgtct tgccgcccac cgacgagtcc
      901 acctgggcaa gcagcgtggt cggcacctga acgaaggcca ccccgcgctg aaacgtggcg
      961 gcaacgaaac ccgtgatgtc cccgaccacc ccgccgccga gggccaccag cgtggttgag
     1021 cggttgtgcc gccgctccat gagaaaatcc atgatggcgc cgtagctttc caggttcttg
     1081 tgctgctcgc catcgggcag ggtgtagacg tcggcatcga ttcccgcgag ggcttgggcc
     1141 acgcgaggca ggtacaaggg ccccaccaca tcgttggtga tcacggcgac ctggctgccg
     1201 tgcacctgat cgcgcagcag ctctccgcgt cccagcaggc cgtcaccaat gtgaatgggg
     1261 taactgcgat cccccaggtc gacctcgagc gtcacgtcag tcaaatcaaa ccatcctcgc
     1321 gcagcttgcg ctcgatcgtg cgggccagca ccttcggacc ctgccggtcg gttacgaatg
     1381 tgtaatcggc tatttccgcg tagagcggag ccctctcgcg ttgcaggcga gcgagggtct
     1441 cgcgggcgtc accgccctgc agcagcggcc tcttgttgtc gcgcgccgtg cgctccacga
     1501 gccgctcgag ggggctgtcg aggtgcacga cgatccctcg agccgacaga ttctgccggt
     1561 tcgcagcacg cagcaccgcg ccaccacccg tcgccagcac gacgccgctt tttgccgtca
     1621 gctcctcgat gacctgctgc tcccgatcgc gaaagcccga ctcgccctcc acgtcgaaga
     1681 tccaggcgac ctcggccccc gcgcgctcct cgaggacctc gtcagtgtcg tagaacgcca
     1741 tgtcgaggtc ccgcgcaaga caacgcccca cggtactttt gcccacagcc atcaaaccaa
     1801 cgagaaagag attctgcgtg cgcataaaaa agcggcgcct gaagaggcgc cgctagttta
     1861 gtttattcgg gctgggcccg cgacgatcac cgggcggaca cggtgtcctg gagaatcttc
     1921 ggcgtgatga agatcagcaa ctcggtcttt tcctcgttct ccagctcttt t
//
LOCUS       DBNHOX010000768         3579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_513902, whole genome shotgun sequence.
ACCESSION   DBNHOX010000768 DBNHOX010000000
VERSION     DBNHOX010000768.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_513902"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /gene="truA"
                     /locus_tag="AC16UZ_16665"
     CDS             complement(<1..535)
                     /gene="truA"
                     /locus_tag="AC16UZ_16665"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR6248373.1"
                     /translation="MHLALGVEYDGSAFHGFQLQKAAATVQGALEQALSRIADHPLRI
                     AAAGRTDAGVHATGQVVGFASNAERPIQAWVKGVNALTPPSVKVRWAREVDPRFHPRY
                     SATARRYMYLWYEDDVTSPTLDRWAVRVRHLDDDAMHAASRSLVGEHDFSAFRASGCQ
                     SESAHRCVHRITVHRHGP"
     gene            complement(535..2766)
                     /locus_tag="AC16UZ_16670"
     CDS             complement(535..2766)
                     /locus_tag="AC16UZ_16670"
                     /inference="COORDINATES: protein motif:HMM:NF051932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="motility hub landmark protein FimV"
                     /protein_id="HIR6248374.1"
                     /translation="MIRSLLSRARAHVISIGAALSLLAAHAWGLGLGEIKLNSALNEK
                     LKAEIELLDASDMQPDEIRVSLASTEDFERVGVERFFFLTDLRFEVAFSSSSGVKVDV
                     TSTKPITEPYLNFIVEVLWPNGRLLKEYTLLLDPPTFSSAAAPVVAPPARTASSGGSG
                     DDVTRTAPSGGTRVSLAPPSPSTPAPRNIGGELMTDQSDTLWEIAQRTRPEGVSIAQD
                     MLAIQKLNPQAFIRNNINLLKAGYSLRLPTAEEARALSQQQAVALVAEQNADWRAYNR
                     GEAPAVETQVAQSVPSEFQGQVDATAERAEPVVESASEGELRIVAGVGDSVSGTAAAE
                     TVAQLDAAREEQDRLSREVDELAYQMDRERELSTNQLAVKDRQLEVKDQQIAELQAQM
                     AQVRQELNEARQNQNQSVPAPEQAKSWWESSYVLGGAAGALVLALVAGLIGARRKRPV
                     EDEVAVAAVTPRAARTSTTAAAAKAATADEGASVQAASGVSGDSGAHDAVPAPGDGDV
                     LAEAEIYIAYGRLPRAISLLQDAIEEEPERDDVRLKLLELYVESGERGAFSAQLTEFM
                     GRCQDEEMRDAARELEARLTGGALDVSQEAPVKAEAAEDEGESNVMDFDLGLDDEPAS
                     TTAVEADGGESEFDLELDEDEGASRGAGDQLGGDLGIDFKPEATALDADEEGSEVATI
                     DEDEFDFDVESDADSAETKLDLAKAYIDMGDEDGARDILKEVLEEGTSDQQTKAQTLL
                     EQL"
     gene            complement(3071..>3579)
                     /locus_tag="AC16UZ_16675"
     CDS             complement(3071..>3579)
                     /locus_tag="AC16UZ_16675"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR6248375.1"
                     /translation="KPIESKVFPVQIAFNALPHIDKFQDNGYTKEEMKMVWETQKILE
                     DEAIRVNPTCVRVPVFFGHSEAVHIETEHKITAEEARRLLAAAPGVTLIDDPSANGYP
                     TAVSHGAGQDAVFVGRVREDVSFDRGLNLWVVSDNVRKGAALNSVQIAEELMARQPQL
                     FAISDGLG"
BASE COUNT          616 a         1177 c         1103 g          683 t
ORIGIN      
        1 ggggaccgtg acgatggacg gtgatccgat gcacacatcg gtgagccgac tcggactggc
       61 acccgcttgc tctgaacgcc gagaaatcgt gctcgccaac gaggctgcgc gacgccgcgt
      121 gcatggcgtc gtcatcgagg tgccgcaccc gcacggccca ccgatccagc gtcggggagg
      181 tcacgtcgtc ctcgtaccat agatacatgt agcgtcgcgc cgtggccgag tagcgcggat
      241 ggaatcgagg atcgacttcc cgcgcccagc gcaccttgac ggatggcggg gtcagggcgt
      301 tcaccccttt gacccaggcc tgaatcggcc gctcggcatt ggaggcgaaa ccgacgacct
      361 gacccgtggc gtgaaccccg gcatcggtgc gcccggcggc tgcaattctc agaggatgat
      421 cggcaatacg cgaaagcgcc tgctccaggg caccctgaac cgtagctgcc gccttctgca
      481 gctggaagcc gtgaaaggca ctgccgtcat actcgacgcc cagcgccagg tgcatcagag
      541 ctgttcgagc agcgtctgcg cttttgtctg ctggtcgctg gtaccttcct cgagaacttc
      601 cttgagaatg tcccgcgcgc cgtcctcgtc acccatgtcg atgtaggcct tcgcgagatc
      661 cagcttcgtc tccgcgctgt cggcgtcgct ttcgacgtcg aaatcgaact catcctcgtc
      721 gatggtggcg acttcgcttc cttcttcgtc ggcatccagc gcggtcgcct cgggcttgaa
      781 gtcgattccg aggtcaccgc cgagctgatc gccggcgccg cgggatgcgc cctcgtcctc
      841 atcgagctcc agatcgaact cgctctcgcc gccgtccgct tcgaccgccg tcgtcgaggc
      901 cggctcatcg tctaggccga gatcgaagtc catgacgttg gactcgcctt cgtcctcagc
      961 agcctccgcc ttaacgggag cctcctgaga gacatcgagc gcgccacccg tcagtcgagc
     1021 ctcgagctcg cgagcggcat cgcgcatttc ctcgtcctgg cagcgcccca tgaactctgt
     1081 cagctgggcg gaaaaagcgc cccgctcgcc ggattcgaca tagagctcga gcagcttgag
     1141 gcgcacgtca tcgcgctcag gctcctcttc gatagcatcc tgcaggaggc tgatggcccg
     1201 cggtaaacga ccgtaggcaa tgtagatctc ggcctcggca aggacatcgc cgtcacctgg
     1261 tgcaggcacc gcgtcatgcg ctccgctgtc cccggacacg cccgacgccg cctggactga
     1321 ggctccctcg tcggctgtgg cggccttggc tgcagcagcc gtggtgctgg tacgcgctgc
     1381 cctgggggtg acggcggcga cagcaacctc gtcctcgacg gggcgcttgc gacgcgcgcc
     1441 gatcaatccg gcaacgagag ccaaaaccaa agcgcccgcg gcaccgccga gaacgtagga
     1501 cgactcccac caactcttgg cctgttccgg cgcgggcacg ctttggtttt ggttctgccg
     1561 cgcctcgttg agttcctggc gaacctgggc catctgagcc tgaagttcgg caatctgctg
     1621 gtctttgacc tcgagctgtc gatcctttac cgccagctgg ttcgtggaaa gctcacgttc
     1681 ccgatccatc tggtaggcca gctcgtcgac ttccctggaa aggcgatcct gctcttcacg
     1741 tgccgcatcg agttgcgcga ccgtctccgc agcggcggtc ccggatacgc tgtcgccgac
     1801 cccggcgacg atccgcagct ctccttccga tgcggattcc acgaccggct cggcccgctc
     1861 tgcggtcgcg tcaacctgac cctggaactc gctgggcaca gactgggcca cctgagtctc
     1921 cacggcgggc gcttcgccac gattgtaggc gcgccagtca gcgttctgct cggccaccaa
     1981 ggcgacggcc tgctgctgag acagcgcgcg cgcctcttcg gcagtgggca gcctcagcga
     2041 gtatccggcc ttgagcaggt tgatgttgtt gcgaatgaac gcctgcgggt tcagcttctg
     2101 gatggcgagc atgtcctgag cgatggacac gccttccggc cgggtgcgtt gagcgatttc
     2161 ccagagcgta tcgctctgat cggtcatcag ctcaccgcca atgttccgcg gagcaggcgt
     2221 cgagggcgac ggcggcgcga gggacacgcg ggtaccgccg gatggggccg tgcgggtcac
     2281 gtcgtctccg gacccgccgg aggacgccgt gcgtgcgggc ggcgccacca ccggagccgc
     2341 cgcgctgctg aacgtgggcg gatcgaggag cagcgtgtac tccttgagca gcctgccgtt
     2401 aggccagaga acctccacga tgaagttgag gtagggctca gttatcggct tggtgctcgt
     2461 gacgtcgacc ttgacgccgg acgaggacga gaacgcgacc tcgaagcgca ggtccgtcag
     2521 gaagaagaac cgctcgaccc caacccgttc gaaatcctcg gtagaggcga gagacacccg
     2581 gatctcgtca ggctgcatgt ctgacgcgtc gagcaactcg atctccgcct tgagcttctc
     2641 gttcagcgcc gagttcagtt tgatctctcc gaggccgagg ccccaggcgt gtgccgcaag
     2701 caaagacaac gcggcaccga tagaaatcac gtgcgcgcgc gcgcgactga gcagactcct
     2761 gatcataggc tagccccttt aacaaaagta atagcgaaat tgatgcggac gcccgttccg
     2821 ccgagtgaat gtctcactgc tatcctgtgc acttcatcaa atccatgtgc caaacgagac
     2881 gatacctcac ttgggacggc caaaaattgc agtaggtaac agtttcgcga tggttttggg
     2941 cgctgagtcc cgaatcggga cgccccgaaa gccgttctgc aggcagcttg ctgcgggatc
     3001 tgaggaaacg actccgacgc ggctggcggg catgaaggat gtgtcggtac ggttaagttg
     3061 ctggagcctt ttaccccaaa ccatcggata tggcaaagag ttgcggctgc ctcgccatca
     3121 gctcctccgc gatctgcacg ctgttgagcg cagcgccctt gcggacgttg tctgaaacca
     3181 cccacagatt cagaccgcga tcgaaggaga cgtcctcgcg caccctcccc acgaacacgg
     3241 catcctggcc cgcgccgtgg ctcaccgccg tcgggtagcc gttcgccgag ggatcatcga
     3301 tcagcgtgac gccgggggct gcggccagca gacggcgggc ttcctccgcc gtgatcttgt
     3361 gctcggtttc gatgtgcacc gcttccgagt ggccgaagaa gacgggcacc ctgacacagg
     3421 ttggattcac ccgaatcgct tcgtcctcca ggatcttctg ggtttcccac accatcttca
     3481 tctcttcttt ggtgtagccg ttgtcctgaa acttgtcgat atgtggcagc gcgttgaatg
     3541 caatctgcac cgggaagacc ttgctctcga tgggcttgc
//
LOCUS       DBNHOX010000769         7152 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_963209, whole genome shotgun sequence.
ACCESSION   DBNHOX010000769 DBNHOX010000000
VERSION     DBNHOX010000769.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_963209"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="AC16UZ_16680"
     CDS             <1..602
                     /locus_tag="AC16UZ_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR6248376.1"
                     /translation="SIFFGLATPTEAAGVGAAGALLLAWINRRLGKGVMTNVLQETTR
                     TTAFIFAVLLGATAFSLVLRGLGGDELIEDALLSLPFEATGILICILLATFVLGFFLD
                     WIELTLIILPLVSPVVTGLGFDPVWFTILFAVCLQTSFLTPPVGFAIFYLKGVAPEGV
                     DVRTIYRGVVPFIVLQLIGLLIIFNWKALVTWLPAQAYS"
     gene            complement(604..1719)
                     /locus_tag="AC16UZ_16685"
     CDS             complement(604..1719)
                     /locus_tag="AC16UZ_16685"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014433.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HIR6248377.1"
                     /translation="MSAALKLEGVVADRRIIICCGSGGVGKTTTGAALGVLAAQLGRS
                     ALVMTIDPAHRLAQAMGLQALGHEPAEIALEAPGRLSAMMLDTKHTFDRLVEQYAPSG
                     QVRDAIFANEYYRQLSSTLGGSRELVAMERVLEAATEEDYDLLIVDTPPAHHALDFLD
                     APRRLIDLIDGSLTNSLLKPYGMAARAQFNLFRQSSAVTLKFLERLTGFEMLADLSEF
                     LLAFSSMFDGFKERSHRVMELMRESGTTFLLICAPEEGSLEQVEQFRSRLEKDSLDIC
                     GVLVNRVHLMDPRAGDLDDSVLARIASLDVAEPRGAFLAERVAAAYEDRRTMARADAG
                     VMADRLGEDLPVQAVPHFHRDLHSMADLQAFAETVRN"
     gene            complement(1716..2630)
                     /locus_tag="AC16UZ_16690"
     CDS             complement(1716..2630)
                     /locus_tag="AC16UZ_16690"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HIR6248378.1"
                     /translation="MAALRGVRLVVVTGKGGVGKTSVAAGIASASARSGLRTVLVTLD
                     TGDTRHPLLDVPLEYQPYQAAPRLSVSRLDALEAVREYVRRNMPFSSLYENIFDGHAF
                     RNFASAAPGFEELMSLGKLYELCTSGEFDRVVFDAPATGHMKLLLDVPGATLRAVHVG
                     PLNHNAGKIQDLLLDGSRTRVVITTLAEEMAVEEAMDLERCCRNDHRMAVGPIVVNRL
                     VPERFSVEEIAALQSMEASTLELREAVVAVVAEHAEQRSQEEALRALSAAGSDVVSVP
                     RIVQACFDPAAFLEALEAHLMPLFGSAA"
     gene            2801..3574
                     /locus_tag="AC16UZ_16695"
     CDS             2801..3574
                     /locus_tag="AC16UZ_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248379.1"
                     /translation="MTTYEVVARNFSQAHENRIHSDEVAQRYGFTGALVPGVAVYGHL
                     THPVVERLGEDWLGRSVSSVRFLKPAYDGDRLHLDCESRGSALLVSCRNDAGTLLAEL
                     HTEQPRDMPEPVTAAVLDEPRRTAERAEISWEAVVENETFEPWHWQITEDGNAQYAAE
                     VADHLPIYRLCAHPHWLLSTANRALTREYAMPAWIHVGSDVRHRALVKVGDTVEVRAT
                     LMEKWRRKGHEFLRLYVAYRRGRELTTEIFHTAIFKVAA"
     gene            3574..4170
                     /gene="purN"
                     /locus_tag="AC16UZ_16700"
     CDS             3574..4170
                     /gene="purN"
                     /locus_tag="AC16UZ_16700"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR6248380.1"
                     /translation="MRIAALASHGGSILQAVIDAIENGTLPAELVLVVSNNSRSQALA
                     RASRHGIATLHLSAHTHRDPDELDAAIVRSLEEANADWVLLAGYMKRLGPRMLGRFRN
                     RIINTHPALLPKFGGQGFYGQRVHEAVIEAGETESGATVHVVNEAYDSGPILAQVRVP
                     VEKNDTAEVLEERVKRAERKLIVTTLAELAARPAASGY"
     gene            complement(4199..4957)
                     /locus_tag="AC16UZ_16705"
     CDS             complement(4199..4957)
                     /locus_tag="AC16UZ_16705"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6248381.1"
                     /translation="MASLELMDANQLDYSPIRRAGVIGDVHAEDERLAGALEWLAGQD
                     VDVILCTGDVADGVGCVNTACSLLRDADVLTVAGNHDRWLLQDRVRHVADAHFLADLD
                     DESRDYLTALPRSRTLNTVAGPLLLCHGVVENDLAKVWPGTERSPIERSTEMDDLVAA
                     STHRFVVNGHMHYRVLIDFEGVVLVNAGTIRGPFSGVTVIDFEGDFAAAYERDGNGRA
                     HRVAEHELTRNGERRIWRDTQEFDGSWQPVTLHP"
     gene            5080..5919
                     /gene="surE"
                     /locus_tag="AC16UZ_16710"
     CDS             5080..5919
                     /gene="surE"
                     /locus_tag="AC16UZ_16710"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014077.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR6248382.1"
                     /translation="MFAFAAQPCTDRPLNILLVNDDGFDTPGIIALHRELKASGHNVR
                     RVAPDRNYSGSSASLTIDLIAAPRIPDEEFTEVYAVSGSPATAVLVGATAIFPPEQPV
                     DLVVSGINEGANLGPATVISGTVGATIAAMKALARPIPGIAVSTNPIAEDEAAPENHR
                     HLKTVAGFTSRLVRRLFCASQPWSKGELALNVNYPPLEPEAVKGVRVAAQGKAPYYSM
                     GFKAAAPDRYAPDFGRAEPSQDIENADTFLFRQGYITIVPIDGDYTAGGRPLPASLED
                     LEP"
     gene            complement(6232..7074)
                     /locus_tag="AC16UZ_16715"
     CDS             complement(6232..7074)
                     /locus_tag="AC16UZ_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR6248383.1"
                     /translation="MLEYSEARQISDSERVEYLEFALGLAAAAGSAILPFFRAGAGVE
                     NKRTDGGFDPVTEADHAAERVIREQILGRYPEHGIFGEEFGLDAGNGLTWVVDPIDGT
                     RAFMSGLLHWGVLLALFDGERPVLGVMHQPFTAEFFAGDCHNAHYHRGETSQALEVRE
                     CKGLGQAVLGTTSPKLFTSPFERRGLDAIEERVKLSRYGGDCYLYAMLAMGQLDIVMD
                     AGLQPYDVQALVPIIEGAGGVVTTAQGGDASMGGFVVATGSAELHAEVLATIRAGSEL
                     GPGS"
BASE COUNT         1391 a         2398 c         2132 g         1231 t
ORIGIN      
        1 gcagcatctt cttcgggctc gcaacgccaa cggaggcggc gggcgtgggc gcggccgggg
       61 ccttgctgct cgcgtggatc aatcgtcggc tgggcaaggg ggtgatgacc aacgtgctgc
      121 aggaaaccac gcgaacgacg gccttcatct tcgccgtgct gctcggcgcc actgctttct
      181 cgctggtcct gcgaggtctc ggcggagatg agctcatcga agacgccctg ctcagcctgc
      241 ccttcgaagc cacgggcatc ctgatctgca tactgctggc aaccttcgta ctcgggttct
      301 ttctcgactg gatcgagctc acgctgatca tcctgccgct ggtttcgccc gtggtcacgg
      361 ggctggggtt cgatccggtt tggttcacga tcctgttcgc cgtctgcctg cagaccagct
      421 ttctaacccc gccggtaggc tttgcgatct tctacctgaa aggcgtggca ccggagggag
      481 tggacgttcg gaccatctac cggggcgtgg tgccgttcat cgttcttcag ctgatcggcc
      541 tgctgatcat cttcaactgg aaggcgctcg ttacctggct tccggcccag gcctacagct
      601 gagtcagttc cgaacggtct ctgcgaacgc ctgaagatcc gccatgctgt gcaggtcccg
      661 atggaaatgt ggcacggcct gcaccggcag gtcttctccg agtcggtctg ccatcacgcc
      721 ggcgtctgcc cttgccatgg tgcgtcgatc ctcataggcc gccgcgactc gctcggccag
      781 gaaagcaccg cgcggttcgg cgacgtccag gctggcgatc ctggcaagca ccgagtcatc
      841 gagatcaccc gcccgcgggt ccatcaggtg cacccggttc accagcacgc cgcagatatc
      901 cagtgaatcc ttctccagac gcgagcgaaa ctgttccacc tgttccaggc tcccttcctc
      961 cggcgcgcag atcagcagaa aagtcgtccc cgactcacgc atgagctcca tgacgcgatg
     1021 ggaccgctcc ttgaaaccgt cgaacatgct ggagaacgcc agcaggaact ccgacagatc
     1081 ggccagcatc tcgaaaccgg tcaagcgctc gagaaacttg agagtgaccg cagatgactg
     1141 ccggaacagg ttgaactgcg cccgcgcagc catgccgtaa ggcttgagca gcgagttcgt
     1201 cagggaaccg tctatcaggt cgatcagacg ccgcggcgcg tcgagaaagt ccagcgcatg
     1261 gtgagcaggc ggcgtgtcga cgatcagcag atcgtagtcc tcctccgtcg cagcttcgag
     1321 aacgcgctcc atggccacga gttcccggga ccctccgagc gtcgaggaga gctggcggta
     1381 gtactcgttg gcgaaaatgg cgtctctgac ctgaccgctg ggcgcgtatt gctccaccag
     1441 ccggtcgaag gtgtgtttgg tatcgagcat catggccgac agcctgccgg gtgcttccag
     1501 cgcaatttcc gcaggttcgt gccccaacgc ctgcaggccc atggcttgag ccagccgatg
     1561 cgccggatcg atggtcatga ccagcgctga tctgcccagc tgggccgcaa gcactccaag
     1621 cgccgccccg gtcgtggttt tgcccacacc gccgctgccg cagcagatga ttatccgccg
     1681 atccgcaacg acgccttcca gcttgagcgc ggcactcatg ccgcgctacc gaacaggggc
     1741 atcaggtgag cctccagcgc ttccaagaaa gccgccgggt caaagcatgc ctgcacgatg
     1801 cgcggcacgg acacgacgtc gctgccggcc gcactcagcg cgcgcaacgc ctcctcctga
     1861 gatcgctgct cggcatgctc ggcaaccaca gccaccaccg cttcgcgcag ctccagcgtg
     1921 ctcgcctcca tactctgcag cgccgcgatc tcctcgactg aaaatcgctc aggcaccaag
     1981 cgattgacga caatcgggcc tacggccatg cgatgatcgt tacggcagca gcgctcgagg
     2041 tccatcgcct cctccaccgc catctcttcc gcgagggtgg tgatgacgac gcgcgtccgc
     2101 gagccgtcca gcagcaggtc ctgaatcttg cccgcgttgt ggttcagcgg tcccacgtgc
     2161 acggcccgca aggtggcccc aggcacgtcc agcagaagct tcatgtgacc ggtggcgggc
     2221 gcatcgaaaa ccacccgatc gaactccccg ctggtgcaga gctcgtagag cttgccgaga
     2281 gacatgagct cctcgaagcc gggtgctgcc gaagcaaaat tacgaaacgc gtgcccgtcg
     2341 aagatgttct cgtagagcga cgagaacggc atgtttcgcc ggacgtactc ccgcactgcc
     2401 tcgagcgcgt ccagccgcga aacggaaagc cgcggggccg cctgataggg ctgatactcg
     2461 aggggaacgt cgagcagcgg atgcctggta tctccggtgt cgagggtgac gagaacggtt
     2521 ctgagccccg acctggcgga agcgctcgcg atcccagcag ccaccgacgt cttgcccacg
     2581 ccgcccttgc cagtcacgac caccaggcgc acgccgcgca gcgccgccac tgaagcatca
     2641 ccattcatcg ccgcactata atgcgctttc accacccgga tcgaccttga ctgcgccgca
     2701 gccgacccat cacagacaga tctgcctaac ggacctagag cttcagaggt atgatcagca
     2761 gacgttgcgg caacgcatcc atcggcacag agggccacag atgaccacct acgaggtcgt
     2821 ggcgagaaat ttctcccagg ctcacgagaa caggatccac agcgacgagg tcgcgcaacg
     2881 ctatggcttt accggtgccc tcgtcccggg cgtcgccgtc tacgggcacc tgacgcatcc
     2941 cgtggtggag cgtctgggtg aggactggct cggccggtcg gtgagctcgg tgcggttcct
     3001 gaagccggcc tacgacgggg acagacttca tctggactgc gaaagccgag ggtccgcgtt
     3061 gctcgtcagc tgccgcaatg acgccggcac cctgctggcg gagcttcata ccgagcaacc
     3121 gcgagatatg ccggagccgg tcaccgccgc cgtgctcgac gagccccgca gaaccgcgga
     3181 acgtgcggaa atcagctggg aggcggtggt cgagaacgaa acgttcgagc cgtggcactg
     3241 gcagatcacc gaggacggca atgcgcagta cgccgccgag gttgccgacc atctgcccat
     3301 ctatcgcctc tgcgctcacc cccactggct tctcagcact gccaatcggg cactcacgag
     3361 ggagtacgca atgccagcat ggattcatgt tggctccgat gtccggcacc gggcgcttgt
     3421 caaggttggc gataccgtgg aagtcagggc cacgctcatg gagaaatggc gcaggaaggg
     3481 ccacgagttc ctgaggctgt acgtggcgta ccgcagaggc agggaactca ccacggagat
     3541 ctttcacacc gcaatattca aggtggcggc ctgatgcgca tcgcggccct ggcatctcac
     3601 ggcggatcga ttctccaggc ggtcatcgac gccatcgaga acggaacgct gcccgcggag
     3661 ctggtgctgg tggtcagcaa caacagcaga tcccaggcgc tggcacgcgc gagccgccac
     3721 ggaatcgcaa cccttcacct gagcgcccat acccatcggg atcccgatga gctcgacgcc
     3781 gccatcgtcc gttcgctgga agaagcgaac gcagactggg tccttctggc cggctacatg
     3841 aagaggctag gcccgcgcat gctcggtcgg ttcaggaaca ggatcatcaa cacccatcct
     3901 gcacttcttc caaagttcgg cgggcaggga ttctacggcc aacgggttca cgaggccgtg
     3961 atcgaggcgg gtgagacgga atcgggcgcc accgtgcacg tagtgaacga agcatatgac
     4021 tccgggccca ttctcgccca ggttcgagtg cctgtcgaaa agaacgacac ggcggaggtg
     4081 ctcgaggagc gagtcaagcg cgcggagagg aagctcatcg tcacgacgct ggccgagtta
     4141 gccgctcgcc cggccgcctc gggatactga ggcaacggga cggcctcgcg gcgcgcctct
     4201 aagggtgcag ggtaaccggc tgccaggaac cgtcaaattc ctgcgtgtca cgccagatcc
     4261 ttcgctcccc attgcgggtc agctcgtgct ccgcaacgcg gtgcgcccgc ccattgccat
     4321 cacgctcgta ggccgccgcg aaatctcctt cgaaatcgat cacggtgacg ccggagaaag
     4381 gccctctgat ggtgccggcg ttgacgagga ccacaccctc aaagtcgatc agcacccggt
     4441 agtgcatgtg tccgttgacc acaaaccggt gcgtgctcgc agcaaccaga tcgtccatct
     4501 ccgtgctacg ctcgatcggc gaacgttccg tgccgggcca gaccttcgcg agatcgttct
     4561 ccaccacccc atgacacaaa aggagcggtc cggcgaccgt attcaaagtc ctcgaacgag
     4621 gcagcgcagt caggtaatcg cggctttcat cgtccagatc ggccagaaag tgggcgtccg
     4681 cgacgtgccg aacgcggtcc tgcagcagcc atcgatcgtg attgcccgcg accgtcagca
     4741 catcagcgtc tctgagcagg ctgcaggcgg tattgacgca gccgacgccg tcggcgacgt
     4801 cgccggtgca caggatgacg tccacgtcct ggcccgcgag ccattccaac gcccccgcca
     4861 gacgctcatc ctcggcgtgc acgtcgccta tcacgccggc gcggcgaatg ggggagtagt
     4921 ccaactggtt ggcatccatg agttcgagac tagccaccga ctcgtgaagt ctccgtgaac
     4981 ggatctaaac ccgtcaccga gatatggcat gatgatctgc ggccatcctc tgtttgcatc
     5041 atgtaccgac gattcttcgc aattgttctg ctgtcaccaa tgttcgcgtt cgcggcccaa
     5101 ccgtgcacgg acaggccgct gaacatactg ctcgtcaacg acgacgggtt cgatacgccc
     5161 gggatcatcg ctctgcatcg cgaactaaaa gccagcgggc acaacgtcag acgggtcgcg
     5221 ccggaccgca actacagcgg cagcagcgca tcgctcacca tcgacctcat cgcggccccc
     5281 cgaatcccgg acgaggagtt caccgaagtc tatgcggtct ccgggagccc tgctacggca
     5341 gtgctggtcg gcgcgaccgc catcttcccc ccggagcagc ctgtggacct ggtcgtttcc
     5401 gggatcaacg agggagcaaa tctggggccg gcaaccgtga tctccgggac cgtgggcgca
     5461 accatcgccg ccatgaaggc gctggcaaga ccgatcccgg gaatcgccgt gagcacgaat
     5521 cccatcgccg aagacgaggc cgcgccggaa aaccatcgtc atctgaagac ggtcgccggg
     5581 ttcaccagcc ggttggtgag gaggctcttc tgtgcttcgc agccctggtc taaaggcgag
     5641 cttgctctga acgtcaacta tcctccgctc gagcccgaag ccgtaaaggg ggtgcgagtc
     5701 gcggcccagg gaaaggcgcc ctactactca atgggcttca aagcggcagc ccccgatcgc
     5761 tacgccccgg acttcggccg ggcagaaccg agccaggaca tcgaaaacgc cgacaccttt
     5821 ctcttccggc agggctatat caccatcgtg cccatcgacg gcgactacac tgccggcgga
     5881 cggccacttc cggcctccct cgaggatctc gagccgtaac ttctgccgtg acggtccagg
     5941 aaccgcagat acgttgaccg agcaccgcgc caaacggtta caaaaccccg acattgtcgt
     6001 cacacttcgg caggcggtcg gtggcagtct agacgctcga ggccccagcc ggttcgcgcc
     6061 gggccctcga ggagcggacc agcagtatcg gtccgctctg atcgacccgt ttagtcgcgt
     6121 tttcgggtca catcagcccg gcgtgccgaa ccgacgatca ggacacggac gtcccctcga
     6181 cattccggcc cgtcgatgac gggccgctga tctgaaccac tcgattcgaa tctaggaacc
     6241 agggccgagt tcactgcccg cacggatggt cgcgagcacc tctgcatgaa gctccgcgct
     6301 gccggtagcc accacaaacc cgcccatgct ggcgtcaccg ccctgagcag tggtcacgac
     6361 cccaccagcg ccctcgatga tcggcaccag agcctgaacg tcataaggct gcaggccggc
     6421 atccatgacg atgtcgagct gacccatggc aagcatcgca tacagatagc agtccccacc
     6481 gtagcggctg agtttcaccc tttcttcaat ggcgtcgagg ccccgccgct cgaatggcga
     6541 cgtgaaaagc ttcggcgacg tcgtgccgag aacggcctgc ccgagcccct tgcactcccg
     6601 gacttcgagc gcctgggagg tttcaccccg gtggtaatga gcgttgtggc aatcgcccgc
     6661 aaaaaactct gcggtaaaag gctgatgcat gacgcccagg acgggacgct cgccatcgaa
     6721 cagcgccagc agcacccccc aatgcagaag gccgctcatg aacgcccgag tgccatcgat
     6781 gggatcaacg acccaggtca gaccgttacc cgcgtctaaa ccgaattctt ctccgaagat
     6841 cccgtgttcg ggataccggc cgagaatctg ctcacgtatc acgcgctccg cagcgtgatc
     6901 ggcctccgtt accgggtcga atccaccatc ggtgcgtttg ttctccacac ccgcgccggc
     6961 gcgaaagaac ggcagaattg cagagccagc cgcggccgcc agccccagag cgaactccag
     7021 atactcgacc cgctccgaat cactgatctg ccgcgcctcg gaatattcaa gcatcgagtc
     7081 gttgggctcc catccagata tcaagatcca cacccgatta tgtacgagtg ttgtactgtt
     7141 cgctccctcg tc
//
LOCUS       DBNHOX010000770         6745 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_255790, whole genome shotgun sequence.
ACCESSION   DBNHOX010000770 DBNHOX010000000
VERSION     DBNHOX010000770.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_255790"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="AC16UZ_16720"
     CDS             complement(<1..511)
                     /locus_tag="AC16UZ_16720"
                     /inference="COORDINATES: protein motif:HMM:NF013428.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR6248384.1"
                     /translation="MRQALLETNLGLPNRRSGKVRDLYDVTLDGGEEALLIIATDRIS
                     AFDVVMANGLPGKGIVLTQISRFWFDHFAGEVDHHLISTDVADVHGLSDAKREALAGR
                     IMLCRKTRVVPIECIARGYITGSGWKDYQRTGAVCGIELPEGLRNSDRLEEPLFTPST
                     KADTGHDENI"
     gene            complement(548..1315)
                     /locus_tag="AC16UZ_16725"
     CDS             complement(548..1315)
                     /locus_tag="AC16UZ_16725"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6248385.1"
                     /translation="MGRLKGKVAVITGGAGGIGAAAGRLFAEEGADVLLVDVDEKALE
                     ETVRGIGSNRVSYFVGDVTASADNAAMVACAEERYGGVDVLIANAGIEGDVAVPIVDY
                     DEARFDQVLGVNVKGVFLGLKSAIPAMARRGGGSIVITSSVAGVGGSPGMSAYVTSKH
                     AVIGLMRSAARECAGQNIRVNTVNPSPVETRMMRSIEEGIAPGGGEQVKSLMQSRIAL
                     QRYAEPEEIAKVMLFLASDDSAWVTGSVYMADGGMTA"
     gene            complement(1342..2565)
                     /locus_tag="AC16UZ_16730"
     CDS             complement(1342..2565)
                     /locus_tag="AC16UZ_16730"
                     /inference="COORDINATES: protein motif:HMM:NF004609.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HIR6248386.1"
                     /translation="MKGFTTKIVHSDRLEVIEHGSLHKPVHSTVAYGYDDARDLAAVF
                     QGTKAGYTYGRQVNPTVEALQTKISTMEQGLQTVCFSTGMAAIGTMLFALLRNGDHFV
                     SSSFLFGNTNSLFNSFSTHGVDVTFVDATDVDAVAAGINERTRLVFVETIANPRTQVS
                     ALEEIGKLCAERGLIYVVDNTMTSPYLFLPASVGASLVVNSLTKYIGGHGNALGGAVT
                     ELGNFDWADFSNIYDTYKSGAAEKWAITQIKKKGLRDFGAALAPEAAHHIAVGSETLG
                     LRLERQCANAAAMTAMLDAHPKVKAVYYPGLKQHPQHERASRLFRHPGALFSFELVPE
                     VDIWNFMNALDVIIKSSNLGDNRTLAIPVAHTIYYEMGPERRASMGIDDSLIRISVGI
                     EESEDLLGDLERALG"
     gene            complement(2584..4836)
                     /locus_tag="AC16UZ_16735"
     CDS             complement(2584..4836)
                     /locus_tag="AC16UZ_16735"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR6248387.1"
                     /translation="MSESSTADASSPRESFSRLLNRKSAGSGLVGASILLVDDKPELL
                     NSLYQLMVLHGYEPDKALGGQQALDLLAEKSYDVVLLDLIMPGVSGHDVLDYAARNEL
                     TCKIIVVSGDSSFSGVKHALHCGAFDFVKKPYEAGELIATMETALRQCQLEAQNELME
                     EKVKESEELHRYIVNSSPDLVYMLDRNGCFTFLNDRVESLLGFSRDELIGKHYTELVD
                     DDHLELARNVFNERRTGDRAVSNAEMRLKSRHARRGARLFHAHAIWTELTAMGVYSDP
                     KERTRENFVGTYGIARDISERKEAQEVISFQAYHDLLTHLPNRALLKDRLSLAITQAR
                     RSKRRLAVMFLDLDRFKIVNDTLGHSMGDRLLKAVANRLQSCLRRGDTLSRFGGDEFT
                     LLLPEVRTRDDVVVIATKILDRLGGPFVIDGLELFVGASIGVAMYPEAGETEEALIQN
                     ADIAMYHVKGRGKNGYQFFSEEMNHKYSTRLSMERELRNGLTQEELVVYYQPQVALKT
                     GEIVGVEALVRWRHPERGLVEPGDFLPMAEETGLITQIDTFVQRRACTDVAGWERAGL
                     GSIRLSVNLSAPQIEQEDFVDNFVADMAAAGLDPSRVKLEITENILMQDMEVVIPKLK
                     QLRQLGVRIAIDDFGTGYSSLSYLQQFPIHTLKIDRSFVGDIRADHGDASIVNAIVAM
                     ARGLNLDLIAEGVENRTQLRYLRSQGCSEVQGFIFSDPVPADQVKLMLQQNPFRAMVL
                     DEQPEAATAV"
     gene            complement(4913..5500)
                     /locus_tag="AC16UZ_16740"
     CDS             complement(4913..5500)
                     /locus_tag="AC16UZ_16740"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6248388.1"
                     /translation="MSVDSELELRWERLGSEYGNDLILFKTRFDRMRNPRNDAVLRRL
                     VLESVDWVNVVALTADGLSVMVRQFRFGVDYTTLETPGGMVDPGEDSLAAARRELLEE
                     TGYGGGDWTYLGAVEPNPAFHDHLCHHWLASGVSLLQEPAPTPGEAIEVTLMSERAVA
                     DAVQQGVIKHALALSALSRVFELWPRPFRHERDPS"
     gene            complement(5497..6621)
                     /locus_tag="AC16UZ_16745"
     CDS             complement(5497..6621)
                     /locus_tag="AC16UZ_16745"
                     /inference="COORDINATES: protein motif:HMM:NF018805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HIR6248389.1"
                     /translation="MRIVTLPALLLTLLIVAPGCQPDEPAGGAMGEGTLRLGAVLGET
                     DAQGFARAEAVVPFAFPGDHGPHPRFRSEWWYLTANVQNEFGAEFGVQFTAFRQALAP
                     GPGPANPWLSNQVFLAHAAVTGVLEQGHWHEERVARGHPGVAGATAEPFRVWVDGWEL
                     AGRNGGFDEQRLSVKADAFAVDLQLDALKRPVLQGDRGLSAKGPEQASYYYSVPRLSA
                     RGTLRIGEVEHQVSGRAWIDREWSTSALSSGQVGWDWFALHLDDSWEIMVYQLRRADG
                     SRDRYDHAVLIDPYGAKHPLGADDFELEPEIFWQDDDGVAWPLQWRLKALDRELRIRA
                     AVPDQRMETSVSYWEGLVGVFDMAGRRTGRGYMELTGYEG"
BASE COUNT         1184 a         2266 c         2069 g         1226 t
ORIGIN      
        1 tgatgttctc gtcgtgaccg gtgtccgcct tggtggatgg cgtgaacagc ggctcttcca
       61 gccgatcgct gtttctcaac ccctcgggca gctcgattcc gcacaccgca cccgtgcgct
      121 gataatcctt ccagccgctg ccggtgatgt agccgcgcgc gatgcattcg atggggacca
      181 cgcgggtctt gcggcacagc atgatgcgtc ctgcgagggc ctctctcttg gcgtcgctca
      241 gcccgtgcac gtctgccacg tcggtggaaa tcagatggtg gtcgacctct ccggcgaagt
      301 ggtcgaacca gaaccgtgag atctgggtga gaacgatgcc tttgccgggc aggccgttgg
      361 ccatcacgac gtcgaacgcg ctgatccgat cggtggcgat gatcagcaga gcctcctcgc
      421 cgccgtcgag ggtgacgtcg tagagatcgc gaaccttgcc tgagcgtcgg ttcggcaaac
      481 cgagattcgt ttccagtagc gcctgtctca tttttccccc atgtccaatc gagtcaaact
      541 ttcaatgtca ggccgtcatc ccgccgtccg ccatgtagac cgagccggtg acccaagcgc
      601 tgtcgtcgct ggcaagaaag agcatgacct tggcgatctc ctccggctcg gcgtatcgct
      661 gcagcgcgat gcgcgactgc atcaggctct tcacctgctc gccgcccccc ggcgcgatgc
      721 cctcctcgat gcttcgcatc atacgcgtct ccacgggaga tggattcaca gtgttgacgc
      781 ggatgttctg cccggcgcat tcccgggcgg ccgatcgcat cagcccgatc accgcgtgct
      841 tgctggtgac gtaagcggac atgcccggag atcctccgac gccggcaacc gaggaggtga
      901 tgacgatgct gccgccgcca cgccgcgcca tggcggggat ggccgatttc agccccagga
      961 agacgccctt gacgttgacg cccaggacct gatcgaacct cgcctcgtcg tagtccacga
     1021 tgggcacggc aacgtcgcct tcgatccccg cattggcaat gagcacgtcc accccgccgt
     1081 agcgctcttc cgcgcaggcg accatggcgg cgttgtccgc agaagcggtg acgtcaccga
     1141 caaagtagct gacgcggttg ctgccgatac cgcgcacggt ttcctccagc gccttctcgt
     1201 ccacatcgac cagcaggacg tcggcgcctt cctcggcgaa aagccgaccc gcagcagcac
     1261 cgatgccacc ggcgcccccc gtgatgacgg ccactttacc cttcagtctt cccatgcctt
     1321 gcgcttcccc cgcacaccga ttcagcccag cgcgcgctcg agatctccca ggagatcctc
     1381 gctctcctcg atgcccacgg atatgcggat cagcgaatcg tctatgccca tgctcgcccg
     1441 ccgctccgga cccatctcgt agtaaatcgt gtgagccacg ggaatcgcca gggtccggtt
     1501 gtcacccagg ttgctggact tgatgatcac atccaacgcg ttcatgaagt tccagatgtc
     1561 cacctcggga accagctcga agctgaacag cgcgcccgga tggcggaaca ggcggctcgc
     1621 ccgctcgtgc tggggatgct gcttaaggcc gggatagtag acggctttga ccttcgggtg
     1681 cgcatcgagc atcgccgtca tggcggccgc gttggcacac tggcgctcca gccgcaaccc
     1741 aagggtctcc gagcccacgg cgatgtgatg agcggcttcc ggggcaagcg cggcgccgaa
     1801 gtcccgcagt cctttcttct tgatctgggt gatggcccac ttctcggcgg ccccggattt
     1861 gtacgtatcg tagatgttgg aaaaatccgc ccagtcgaaa ttcccgagct ccgtcaccgc
     1921 tccgcccagc gcgttgccgt gaccgccgat gtacttggtc aggctgttca ccaccaggct
     1981 ggcgccgacg ctggccggca gaaacaggta gggcgacgtc atggtgttgt ccaccacgta
     2041 aatgaggccg cgctcggcgc acagcttacc gatctcctcc aacgcagata cctgcgtgcg
     2101 cgggttggca atggtttcca cgaacacgag ccgggtacgc tcgttgatgc cggcggccac
     2161 ggcgtccaca tcggtggcat cgacaaacgt gacgtccacc ccgtgggtcg aaaaggagtt
     2221 gaacaggctg ttggtgttgc cgaacaggaa ggagctcgac acgaagtgat cgccgttgcg
     2281 aagcagggcg aagagcatgg tcccgatggc agccatgccc gtggagaaac agacggtctg
     2341 cagcccctgt tccatggtgg agatcttggt ctggagggct tccacggtgg ggttcacctg
     2401 ccggccatag gtgtagccgg ctttggtgcc ctggaacacc gccgcaaggt cgcgggcgtc
     2461 gtcgtacccg tatgccaccg tggagtgcac cggcttgtgc agcgagccgt gctcgatgac
     2521 ctcgagccgg tcgctgtgca cgatcttcgt ggtgaaaccc ttcatcggta gcagcgcgcc
     2581 gtgctagacg gctgtggcgg cttctggctg ctcgtccagg accatggccc ggaacggatt
     2641 ctgctggagc atcagcttga cctgatctgc cggcaccggg tcactgaata tgaagccctg
     2701 cacctcgctg cagccctggg aacgcagata gcgaagctga gtgcggttct ccacgccctc
     2761 ggcaatgagg tccaggttga gaccccgcgc catggcgacg atggcgttga cgatggaggc
     2821 atcgccatgg tctgcgcgga tgtcgcccac gaagctgcgg tcgatcttca gcgtgtggat
     2881 ggggaactgc tgcaggtagc tcagcgacga gtatccggtg ccgaaatcgt cgatggcgat
     2941 ccgcaccccg agctgccgca gctgcttgag cttgggaatg accacctcca tgtcctgcat
     3001 caggatgttc tcggtgatct ccagcttgac cctggaggga tccaggccgg cagcagccat
     3061 gtccgcaacg aaattatcga cgaagtcctc ctgctcgatc tgcggggcag acaggttcac
     3121 ggacagccgg atacttccaa gccccgcgcg ctcccagccg gcaacatcgg tgcacgcccg
     3181 gcgctgaacg aaggtgtcga tctgggtgat cagaccggtt tcttcggcca tgggcaggaa
     3241 gtcgccgggc tctaccagcc cgcgctccgg atgccgccac cggacgagcg cctcgacacc
     3301 gacgatctcc ccggtcttca gcgccacctg aggctggtag tacacgacca gctcttcctg
     3361 ggtaagcccg ttgcgaagct cgcgctccat ggacagccgg gtcgagtact tgtggttcat
     3421 ctcctcggag aagaactggt agccgttctt gccgcgaccc ttgacgtggt acatggcaat
     3481 gtcggcgttc tggatcaacg cttcctcggt ctccccggcc tccgggtaca tggctacgcc
     3541 gatgctggca cccacgaaca gctcgaggcc atcaatgacg aagggaccgc ccagccggtc
     3601 gaggatcttg gtggcgatga ccaccacgtc gtcgcgcgtg cgcacctccg gcagcagcag
     3661 cgtgaactcg tcgccgccga agcgcgacag cgtgtcgccg cgccgcagac agctctgcag
     3721 gcgattggct accgctttga gaagccgatc gcccatgctg tggcccaggg tgtcattgac
     3781 gatcttgaag cggtccagat ccaggaacat cacggccagt cttcgcttgc tccgccgtgc
     3841 ctgggtgatt gccagcgaca ggcggtcctt gagcagagcg cggttcggca gatgggtgag
     3901 cagatcgtgg taggcctgaa agctgatgac ctcctgggcc tctttgcgtt cgctgatatc
     3961 ccgcgcgatg ccgtaggttc caacgaagtt ttcccgggta cgctccttgg gatcggaata
     4021 aacgcccatg gccgtgagct cagtccagat ggcgtgggca tgaaacagac gcgcgccccg
     4081 tcgggcgtgg cggctcttca ggcgcatctc cgcgttggac accgccctgt cgcccgtgcg
     4141 ccgctcgttg aacacgttcc gcgcaagctc caggtggtcg tcgtccacca gctccgtgta
     4201 gtgcttgccg atgagttcgt cacgggagaa tcccagcaga ctttcgacac ggtcgttgag
     4261 aaaggtgaag cagccgttgc ggtcgagcat gtagaccaga tccggggagc tgttgacgat
     4321 gtagcgatgc agctcctctg attccttgac cttctcttcc atgagctcgt tttgagcctc
     4381 gagctggcac tgtcgcagcg ccgtctccat ggtcgcgatg agctcgcccg cctcataggg
     4441 cttcttgacg aagtcgaagg ccccgcaatg cagcgcgtgc ttgacgccgc tgaagctgga
     4501 atcgccgctg acgacgatga ttttgcaggt gagctcgttg cgggccgcgt agtcgaggac
     4561 gtcgtgcccg ctcacgcccg gcatgatcag gtccagcaga acgacgtcgt agcttttctc
     4621 cgccagaaga tccagcgcct gctggccgcc gagggctttg tccggctcgt agccgtgcag
     4681 caccatcagc tggtaaaggc tgttgagcag ctcgggtttg tcatcgacga ggagaatcga
     4741 cgcacccacc agcccggacc cggcgctttt tcgattgagc aatcgggaga atgactctcg
     4801 gggactggaa gcgtccgccg tgctgctttc actcatacca gaactgtccc acgtcgtgct
     4861 cgacatgctt accaattgtg acgaattttt gctgctctgg ataggcgccc gctcatgacg
     4921 gatcacgctc gtgccgaaac gggcgcggcc agagctcgaa cacgcgcgac agcgcggaaa
     4981 gcgccagcgc gtgcttgatc accccttgct gcaccgcgtc ggcgaccgcc cgctcgctca
     5041 tcagcgtcac ctcgatggct tcgcccgggg tcggcgccgg ctcctgcagc aacgaaacgc
     5101 cggaggccag ccagtgatgg cagaggtggt cgtgaaaagc gggattcggc tcaacggcgc
     5161 cgaggtaggt ccaatcccca ccgccgtaac cggtctcctc caggagctcc cgccgggcgg
     5221 cggcgagcga gtcctcgccc gggtccacca tgcctcccgg agtctccaaa gtcgtgtaat
     5281 ccacgccgaa gcggaactgg cgaaccatga cggagagccc atcggccgta agcgctacga
     5341 cgttcaccca gtccacggac tccagaacca gccgccggag caccgcatcg ttgcgcggat
     5401 tgcgcatcct gtcgaagcgg gtcttgaaca gaatcaggtc attgccgtac tccgagccca
     5461 gacgctccca gcgcagttcc agctccgaat ccacactcat ccttcgtacc ccgtcaattc
     5521 catgtagccg cggccagtgc gtctgcccgc catgtcgaag acgcccacca gcccttccca
     5581 gtaggaaacg ctggtctcca tgcgctgatc gggaacggcg gcgcggatgc gcagctcgcg
     5641 atcgagcgcc ttcaggcgcc actgcagcgg ccaggcaacg ccgtcgtcgt cctgccagaa
     5701 gatctccggc tcgagctcga agtcgtcggc gccaagagga tgcttcgcgc cgtaggggtc
     5761 gatgagcacg gcgtgatcgt agcgatcccg ggacccatcc gcacgcctga gctggtagac
     5821 catgatctcc cagctgtcgt cgaggtgaag ggcgaaccag tcccaaccca cctgcccgga
     5881 cgagagcgcg ctggtgctcc actcgcggtc gatccaggcc cggccgctga cctgatgctc
     5941 tacctcaccg atcctgagcg tgccccgcgc acttagccgc ggcaccgagt agtaatagga
     6001 agcctgctcc ggccccttgg cgctcaggcc gcgatcaccc tgcagaacgg gccttttcag
     6061 cgcatccagc tgcaggtcga cggcgaacgc gtcagccttc acgctcaacc tctgctcgtc
     6121 gaagccgccg ttccggccgg caagctccca gccgtccacc cagacgcgaa atggctctgc
     6181 ggtggcaccg gcgacgcccg ggtggccacg cgccacgcgc tcctcatgcc agtgcccctg
     6241 ctccaggacg ccggtaaccg ctgcgtgcgc cagaaagacc tggttggaca gccacgggtt
     6301 ggctggcccg ggcccgggcg ccagcgcctg gcgaaacgcg gtgaactgca ccccgaactc
     6361 cgcgccgaac tcgttctgga cgttggcggt cagataccac cactcactcc tgaacctcgg
     6421 gtgagggccg tggtccccag ggaacgcgaa cggcacgaca gcctccgccc gggcaaaacc
     6481 ctgcgcgtcc gtctcgccca gcacggcgcc gagccgcaga gtcccttccc ccatggcgcc
     6541 gcccgccggc tcgtccggtt ggcaaccggg ggccacgatc agcagcgtca gcagcaacgc
     6601 gggcagggtt acgatgcgca ttccctatca gatctccccg gccgaatccc gcggcgacgg
     6661 caacagtccc gccagcacgg cagcaccgat cccgagcgcg agtggcacgg ccacgtcgct
     6721 caatgccaga tgcagatcca ccgtc
//
LOCUS       DBNHOX010000771         2459 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1604973, whole genome shotgun sequence.
ACCESSION   DBNHOX010000771 DBNHOX010000000
VERSION     DBNHOX010000771.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1604973"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="AC16UZ_16750"
     CDS             complement(<1..391)
                     /locus_tag="AC16UZ_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248390.1"
                     /translation="MNRREFLEILGAASITAQLSGCTSLRTARPDDYYALPAWGDARL
                     LHITDTHAQLNPIYFREPSINLGVGAARGQLPHLVGRNLLDVLTSTNPRDVHAFTCID
                     FSEAAERYGRVGGFAYVKTLIDTLRGES"
     gene            complement(388..786)
                     /locus_tag="AC16UZ_16755"
     CDS             complement(388..786)
                     /locus_tag="AC16UZ_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248391.1"
                     /translation="MINYLSKIGVLSLVGILCAGCAEPGQESVADATDGPAAVQRAEP
                     PEGLDPAARATWLIEAAQAAYADSQAAGHAWVTASRALEDARTAAARGDFASAGAQGE
                     KALRLAEASLAQARTEEGAWQDRFPKPPAQ"
     gene            complement(783..1529)
                     /gene="soxA"
                     /locus_tag="AC16UZ_16760"
     CDS             complement(783..1529)
                     /gene="soxA"
                     /locus_tag="AC16UZ_16760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HIR6248392.1"
                     /translation="MALRDYYAGRFPQIALEDHRDGVYAIDAGAREQWMEMEEFPPYE
                     FAVDEGAELFSTPFTSGAGYSDCFGDEGDVAGQFPHFSEEEATVVTLPMAVNACRDSH
                     GEAPLAYEGAEMARLTAYIAYASRGQQINVPEPASAAAAAAYQAGKRFYATRRGQLNF
                     ACTSCHVQLAGNRLRAERLSASLGHVTHWPVYRFKWEDVGSLHKRFQECNSQVGAEPF
                     DEQSEPYRNLEYFLAYMSNGLELNGPATRK"
     gene            complement(1646..1957)
                     /gene="soxZ"
                     /locus_tag="AC16UZ_16765"
     CDS             complement(1646..1957)
                     /gene="soxZ"
                     /locus_tag="AC16UZ_16765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HIR6248393.1"
                     /translation="MANDVKIRGKITDGVATVKSLMVHPMETGTRRDADGNLIPAHYI
                     EKVAAYKNDQEVLTADWGASISKNPFLSFRVKNAAPGDVIRITWVDNTGATGAGEAVL
                     K"
     gene            complement(1982..2428)
                     /gene="soxY"
                     /locus_tag="AC16UZ_16770"
     CDS             complement(1982..2428)
                     /gene="soxY"
                     /locus_tag="AC16UZ_16770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HIR6248394.1"
                     /translation="MALNRRKWITSMATAGAAALLPLRLLADTAAAFKAADANTALKQ
                     LFGDLPVEESTAIEFKIPDIAENGAVVPVTVSTDMPGVRNISIVVDENPNPLSASFNI
                     SPEAFADVSTRVKMGNSSVVHAYVRTADKVYTTSKEVKVTIGGCGG"
BASE COUNT          374 a          772 c          832 g          481 t
ORIGIN      
        1 cggactcgcc gcgcagcgtg tcgatcagcg tcttcacgta ggcgaaacca ccgacccgac
       61 catagcgctc ggcggcctcg gaaaagtcga tgcaggtgaa cgcgtgaacg tcgcgggggt
      121 tcgtcgaagt aagcacgtcg agcaggttgc gacccaccag gtgaggcagc tggccgcgcg
      181 ctgcgcccac gccgaggttg atgctgggtt cccggaagta gatgggattg agctgtgcgt
      241 gggtgtcggt gatgtgcagc aggcgggcgt cgccccaggc cgggagcgcg tagtaatcgt
      301 cgggccgtgc ggtgcgaagc gacgtgcagc cggaaagctg agcggtgatt gacgccgcgc
      361 cgaggatctc caggaattct cggcggttca ttgggctggg ggcttgggaa agcggtcctg
      421 ccaggcgcct tcttcggtgc gcgcctgcgc cagagatgcc tcggccagac ggagcgcttt
      481 ctcgccctgg gcgcccgcgc tggcgaaatc gccccgggcg gccgccgtgc gcgcgtcttc
      541 gagggcgcgg ctggcggtca cccacgcgtg ccccgcggcc tgagaatcag cgtaggccgc
      601 ctgggcggcc tcgatgagcc acgttgctcg cgcggcggga tccagaccct caggtggctc
      661 ggctcgctgg accgcagccg gtccgtctgt ggcgtcggct accgattcct gcccaggctc
      721 cgcgcagccg gcacacagaa tgccgacgag ggagagaact ccgattttgc tcaggtagtt
      781 gatcatttgc gcgttgccgg tccgttcagc tcgagcccgt tgctcatgta ggcgagaaag
      841 tactccaggt tccggtaggg ctcgctctgc tcgtcgaacg gttccgcacc cacctggctg
      901 ttgcattcct ggaagcgctt gtggaggctg ccgacgtcct cccatttgaa gcggtagacg
      961 ggccagtggg tcacatggcc cagcgacgcg ctcaaccgct ccgcccgcag ccggttgccg
     1021 gcaagctgta cgtggcagct cgtgcacgcg aaattcagct ggcccctgcg cgtggcgtaa
     1081 aagcgtttgc cggcctggta ggcggctgcc gccgccgcgc tggcaggctc aggcacgttg
     1141 atctgctgac ctctcgaggc gtaggcgatg taggcggtga gcctggccat ctccgcgcct
     1201 tcgtaggcaa gcggtgcctc cccgtgacta tcccggcagg cgtttacggc catgggcagc
     1261 gtgaccacgg tcgcttcctc ctcgctgaaa tgcgggaact gaccggccac gtccccctcg
     1321 tcaccgaagc agtcgctgta accggctccg ctggtgaagg gcgtggaaaa cagctcggca
     1381 ccctcgtcga cggcgaactc gtatggcggg aactcttcca tctccatcca ctgctcgcgc
     1441 gcgccggcgt cgatggcgta aacaccgtcc ctgtgatctt ccagcgcgat ctgcgggaag
     1501 cggccggcgt agtagtcgcg cagggccatg cgatcgctct ccggatccgc gcttgctcct
     1561 gagcaggaaa cgagcgcgag cacggcaatg gccgtgggcc gccgccaagc gacgggcctc
     1621 ggtccgcgtc ggcgttcagg cgcgctcact tgagaaccgc ttcgccggcc ccggtggctc
     1681 cggtgttgtc cacccaggtg atgcggatga cgtccccggg cgccgcgttc tttactctga
     1741 aggagaggaa gggattcttg gagatggaag ctccccagtc cgcagtcagc acttcctgat
     1801 cgttcttgta ggcggcgacc ttctcgatgt agtgggcggg gatcaggttg ccgtcggcgt
     1861 cccggcgcgt cccggtttcc atcgggtgca ccatgagaga ttttacggtc gctacgccgt
     1921 ctgtgatctt gccgcgaatt tttacgtcat ttgccatgtc gtcagtttcc tcgcttgtcg
     1981 ctcagccgcc gcagccgccg atggtgacct tcacctcttt gctcgtcgtg tagaccttgt
     2041 ccgcggttcg cacgtaagcg tgaacgacgg agctgtttcc catcttgacc cgggtggata
     2101 cgtcggcgaa ggcttccgga gatatgttga agctggccga caacgggttg gggttctcgt
     2161 ccacgactat gctgatgttc cgaacgccgg gcatgtccgt ggataccgtc acggggacga
     2221 cggcgccgtt ctcggcgatg tcggggattt tgaactcgat ggcggtactc tcttccaccg
     2281 gcagatcgcc gaagagctgc ttgagcgccg tgtttgcatc cgcggccttg aaagcggctg
     2341 cggtatccgc gagcaggcga agcggcagca gcgctgcggc gccggccgtg gccatgctcg
     2401 tgatccattt gcgtcgattt aaagccatag cttcctcgtg gttttacagt gagagaacg
//
LOCUS       DBNHOX010000772         1578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_577295, whole genome shotgun sequence.
ACCESSION   DBNHOX010000772 DBNHOX010000000
VERSION     DBNHOX010000772.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_577295"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..330
                     /locus_tag="AC16UZ_16775"
     CDS             133..330
                     /locus_tag="AC16UZ_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248395.1"
                     /translation="MTEKPVQTALGPMDRKRLSTLKETLDHEDDDSIIMFEGRQLGVL
                     LGRQIVDYVEVELGKRNTRGL"
     gene            343..822
                     /locus_tag="AC16UZ_16780"
     CDS             343..822
                     /locus_tag="AC16UZ_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248396.1"
                     /translation="MTRFIDEYASGSDLRDWARDVMSEPPHFTIGGDYMQRWFIIPRN
                     DRMNLYLHRTLRSDEDVMHDHPWDNTSFVIDGGYREYTPNGVIDRKPGDIVHRKADDV
                     HRLELWHGQPSVSLFMTGPKVREWGFHCPKGWVHWKDFTGGYHDGRSEKGAGCGEYA"
     gene            806..1378
                     /locus_tag="AC16UZ_16785"
     CDS             806..1378
                     /locus_tag="AC16UZ_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248397.1"
                     /translation="MANTPEEKLPPPSRRKDRKCNECPTMIRRDNKSGLCTRCVKKSN
                     EFRRAASGAAHRRYEDPAERERTGAAVKRANQLDPTIRVRKSQIMKEIAATPEWKARN
                     AQQCRDRRLWEVGVAARTPESDARAGRTFSQRHGIASWCPLEYIEQARELRKAGVSVE
                     ETKRMIAEQQEADLERYRRKMGSRWGGDGE"
     gene            1357..>1578
                     /locus_tag="AC16UZ_16790"
     CDS             1357..>1578
                     /locus_tag="AC16UZ_16790"
                     /inference="COORDINATES: protein motif:HMM:NF023101.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger-containing protein"
                     /protein_id="HIR6248398.1"
                     /translation="MGRRRRVNPKQAAHEAFDPLWRDEKLVDRRTAYKLLQIVLDVPV
                     WQSHMRHAPSRVVKRVPAASRRLRKLLRGC"
BASE COUNT          332 a          472 c          533 g          241 t
ORIGIN      
        1 ctcaggcgtt caaggacagc ggcgagttcg agatgtcgaa cgccgtcatg cagggcggct
       61 atgacgagca tcccgagttc cagcggaccc tccgggtcct cgatctgacg ttcaaggtga
      121 aaggcgcacg aaatgactga gaagccggtt caaacggcgc ttggccctat ggaccggaag
      181 cgcctgagta cgctaaaaga aacgctcgat cacgaggacg atgattcgat catcatgttc
      241 gagggacgcc agctaggcgt actcctcggt cgtcagattg tcgattacgt cgaggtcgag
      301 cttggcaagc gcaacacgag aggtctctga tccatgttcg acatgacgcg attcatcgac
      361 gagtacgcca gcgggagcga tctgcgggac tgggcgcggg acgttatgtc cgagcctccg
      421 catttcacca tcggcggcga ttacatgcaa cgctggttca tcattccccg gaacgaccgc
      481 atgaacctct atcttcaccg gacgctccgg tcggacgagg acgtgatgca cgatcacccg
      541 tgggacaata cctcgttcgt gatcgacggc ggctaccggg aatatacgcc gaacggcgtg
      601 atcgaccgca agccgggcga catcgttcac cgcaaggcag atgacgttca ccggctggaa
      661 ctctggcacg gtcagccgtc cgtcagcctg ttcatgaccg gcccgaaggt ccgcgagtgg
      721 ggcttccatt gcccgaaagg ttgggtccac tggaaggact tcaccggagg ctatcacgac
      781 ggacggtcgg aaaagggagc ggggtgtggc gaatacgcct gaggagaagc tacccccgcc
      841 tagccgacgc aaggaccgca agtgcaatga gtgcccgacg atgatccgcc gggacaacaa
      901 gagcggtctc tgtacccggt gcgtgaagaa gtccaatgag ttccggcggg ctgcctccgg
      961 ggcggcccat cggcgctacg aggacccagc cgagcgcgag cgcaccgggg cggcggtcaa
     1021 gcgggcgaac cagttggacc cgacgatccg cgtccgcaag tcgcagatca tgaaggagat
     1081 cgccgcgacc ccggagtgga aggcgcgaaa cgcccagcag tgtcgcgacc ggcggctctg
     1141 ggaggtcggc gtagcagccc gcacccccga atcggacgcc cgtgccgggc ggaccttctc
     1201 ccagcgccac gggatcgcct cgtggtgccc tctggagtat atcgagcagg cccgagagct
     1261 acgcaaggca ggggtgtcgg tcgaggagac caagcggatg atcgccgagc agcaggaagc
     1321 cgatctggag cggtacaggc gcaagatggg gtcgagatgg ggcggcgacg gcgagtaaac
     1381 ccgaaacagg cggcccacga ggcgttcgat ccgctctggc gcgacgagaa gctggtagac
     1441 cgaaggacgg cctacaagct cttgcagatc gtgctagacg tgccggtctg gcagtcgcac
     1501 atgcgacacg cgcccagccg ggtcgtaaaa cgggtccctg cggcgtcacg gcggctcagg
     1561 aagctcctca gagggtgc
//
LOCUS       DBNHOX010000773         2426 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1734379, whole genome shotgun sequence.
ACCESSION   DBNHOX010000773 DBNHOX010000000
VERSION     DBNHOX010000773.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1734379"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="AC16UZ_16795"
     CDS             complement(<1..1514)
                     /locus_tag="AC16UZ_16795"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6248399.1"
                     /translation="MYTAISWFTRNPVAANLLMFVLLIGGCLALFNVHQEEFPSMDVR
                     VVNVTVPYLGAAPEEVEEGVCIRVEEALEGTEGIEKLLSAAREGSCSVQAMLYEDANQ
                     TEALNEIKSQVDGINTFPLETEKPIVSKLTITSDVLQIAVYGDTDERTLKELGKELRD
                     ELSMIDGISKIALNYVRPYEISIEISEDTLRRYGITLEQVARVIRNTSMDMPGGTIKT
                     KGGEILLRAKGQAYWGEEFEDIVVVTRNDGTKVMLSEIAEIRDEFEEGDMRARFNGYP
                     AAVVKVWQVGKEDALEMAADIKAYLTRYEKTLPAGLFVKVWNDESEQLKARLDTLNGM
                     ALSGLGLVVIILAMFLKFRLAMWVAAGIPIALLGTLAVFPYADISMSAMTVMAFILVL
                     GILVDDAIVVGERVYGHEQMGKPPVQAAIDGTWEVSIPVIFGVLTTMAAFLPLLLSTG
                     RMAGFFSVVGWVVIIALVFSIVESQLILPSHLAHRNNDEASNSFSRRWNRIQGRL"
     gene            complement(1519..>2426)
                     /locus_tag="AC16UZ_16800"
     CDS             complement(1519..>2426)
                     /locus_tag="AC16UZ_16800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6248400.1"
                     /translation="LVNGGYFEQGDVLLRLEATDYRSALTRAEAHLDRAAAEFEHAQY
                     EYQRLKSLEVRQLASKSQMENSLRAFRVAEASLQEARAAMEQAMRDLERTALTAPFDG
                     LVRREAVDVGQFVSRGSPVATVYASDQAEVRLPIADRQLAFLNLPVDHRGELPAEQQP
                     RVTLLAEYAGRSLEWQGTIVRTEAQIDTASRMVHVVARISNVEQPVPLNVGLFVNAEI
                     EGLLADNIVVLPRTALRDGDRVLVVDGGNNLRYRNIEPLRLYKDEVLIKAGLAAGERV
                     CVSPLQTAVDGMPVQPVTENAPTAG"
BASE COUNT          409 a          789 c          780 g          448 t
ORIGIN      
        1 aggcggccct ggatgcggtt ccagcgccgg gagaaagaat tggacgcctc gtcgttattg
       61 cggtgggcga ggtgagacgg aaggatcagc tgagactcga cgatgctgaa caccagggcg
      121 atgatgacga cccagcccac gacgctgaag aagccggcca tccggccggt gctgagcagc
      181 aggggcagga aggcggccat cgtggtcagc accccgaaga tcacgggaat cgagacctcc
      241 caggtgccgt cgatggccgc ctgcaccggc ggcttgccca tctgctcgtg accgtatact
      301 cgctcgccga cgacgatggc gtcgtccacc aggatgccca gaaccaggat gaatgccatg
      361 acggtcatgg cgctcatgga gatgtccgca tagggaaaca cggcaagcgt gccgagcagc
      421 gcgatgggga tgccggccgc cacccacatg gccagcctga atttcaggaa catggcgagg
      481 atgatgacca cgagcccgag cccggaaagc gccatgccgt tcagcgtgtc caggcgggcc
      541 ttcagctgct cggactcgtc gttccacacc ttcacgaaca gcccggccgg cagcgtcttc
      601 tcgtagcggg tcaagtacgc tttgatgtcg gccgccatct cgagcgcgtc ttccttgcca
      661 acctgccaga ccttgaccac ggcggcgggg tatccgttga agcgggcgcg catgtcgccc
      721 tcttcgaatt cgtcgcggat ctcggcgatc tcgctcagca tcaccttggt gccgtcgttg
      781 cgcgtgacga ccacgatgtc ttcgaactcc tcgccccagt aggcctggcc cttggcccgc
      841 aggagaatct ctccgccctt ggtcttgatg gtgccgccgg gcatgtccat ggacgtgttg
      901 cggatcaccc gggccacctg ctccagggtg atgccgtacc ggcgcagcgt atcctccgat
      961 atctcgatgg agatctcgta gggccgcacg tagttgagcg cgatcttgga gatgccgtcg
     1021 atcatggata gctcgtcgcg cagctccttg ccgagctctt tgagggtgcg ctcgtccgta
     1081 tcgccgtaga cggcaatctg caggacgtcg ctggtgatcg tcagcttcga gacgatgggc
     1141 ttttccgtct cgagcggaaa cgtgttgatg ccgtcgacct ggcttttgat ctcgttcagc
     1201 gcttccgtct ggttggcgtc ctcgtagagc atggcctgca cgctgcagga gccctcgcgc
     1261 gcggcggaca gcaacttctc gatgccctcg gtgccttcaa gggcctcctc gacccggatg
     1321 cacacgcctt cctccacctc ctcgggcgcc gcgccgaggt agggcacggt gacgttcacg
     1381 acgcgaacgt ccatgctggg gaactcttcc tgatggacgt tgaaaagcgc caggcacccg
     1441 ccgatcagca ggacgaacat cagcaggttc gcggcaacgg ggttcctcgt gaaccaggaa
     1501 atcgcggtgt acatgggctc agccggctgt cggggcgttc tctgtgacgg gttggaccgg
     1561 catgccatcg acggcggtct gcaggggaga tacgcagacg cgctcgccgg cggccaaccc
     1621 cgccttgatc agcacctcgt ccttgtacag gcgcaggggc tcgatattcc gatagcgcag
     1681 gttgtttccg ccatccacca ccagcactcg atcgccgtcg cgcagcgccg ttctcggcaa
     1741 aacgacgatg ttatcggcca gcagcccctc gatctccgcg ttgacgaaca agcccacgtt
     1801 gagcggcacc ggctgctcca cgttggaaat ccgggcaacg acgtgaacca tgcggctcgc
     1861 cgtgtcgatc tgggcttcgg ttcgaacgat ggttccctgc cattccaggc tgcggccggc
     1921 gtactcggca agcagcgtaa cccgcggctg ctgctcggcg ggaagctcgc cgcggtgatc
     1981 cacgggcagg ttgaggaacg ccagctggcg gtcggcgatg ggcagccgga cctccgcctg
     2041 gtcgctggca taaacggtcg ccaccggaga gccgcggctg acgaattgcc cgacgtccac
     2101 ggcttcgcgt ctaaccaggc cgtcgaaggg ggcggtgagg gcggttcgtt cgaggtcgcg
     2161 catcgcctgc tccatggcgg cgcgcgcctc ctggagggat gcttcggcaa ccctgaacgc
     2221 gcgcagggag ttctccatct gggatttgct cgccagctgg cgtacctcca ggctcttcag
     2281 ccgctggtac tcgtactgcg cgtgctcgaa ctccgccgcg gccctgtcca gatgggcttc
     2341 cgccctcgtc aacgccgagc ggtagtcggt ggcttccagg cgcagaagca cgtcgccctg
     2401 ctcgaagtaa cctccattga ccagtg
//
LOCUS       DBNHOX010000774         2511 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_62497, whole genome shotgun sequence.
ACCESSION   DBNHOX010000774 DBNHOX010000000
VERSION     DBNHOX010000774.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_62497"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="AC16UZ_16805"
     CDS             <1..370
                     /locus_tag="AC16UZ_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248401.1"
                     /translation="IALAEGRELDDALEALREVIPDRGTSVANLISAVNELNPPPDNV
                     YLIIDSLPTQGTREPRRATISGGDRLKLFADAIQTLPKQVPINVIMFPMEGDPMAPAA
                     YWNLARISGGAFLSPSRDWP"
     gene            367..1359
                     /locus_tag="AC16UZ_16810"
     CDS             367..1359
                     /locus_tag="AC16UZ_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6248402.1"
                     /translation="MKTRNRRAIEEFSVSFLDVICCGFGAIILLLMITKTVEPILLEK
                     SQVNLEGQVADREQALFEIQGQIKELRRQVDDTSGQLDDNLLELARLEQQLTIILGKF
                     ETTAARQDDNLTETQKLAAAKQSLTDEMQRLLGVDFRRSTDLVGGIAVDSEYIIFVID
                     TSGSMYNAAWPQVLKKVNETLSIYPRVKGIQVMNDMGDYMFARFAGEWIPDSPSRRKA
                     ILDRLQTWNPFSNSSPVEGIQAAINTYYDPDKRISIYVFGDDFTGESIESVVDAVDRV
                     NVADDSGRRRVRIHAVAFPVYLSRPSGRVYRFAALMRELAYRNDGTFVGLSEFQ"
     gene            1524..2072
                     /locus_tag="AC16UZ_16815"
     CDS             1524..2072
                     /locus_tag="AC16UZ_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248403.1"
                     /translation="MTVPTNFPVPVLDKLPLNMGIYLSDDLLNYVHAERLENAGSWQI
                     SLGDAQPAMFNNLLSGMFQGVETILDPSAPSAGVNGVLVPSIQEVQFSIPSQTRSDYY
                     EVWIRYQFQLYDGEGQLLADWPLTAYGKADEDNYGLSTDQPALQAAALAACRDAMAFF
                     TVQFRDVPSVKAWLDRQLGGTS"
     gene            2069..>2511
                     /locus_tag="AC16UZ_16820"
     CDS             2069..>2511
                     /locus_tag="AC16UZ_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248404.1"
                     /translation="MMGFHRIIALAALAAVATVLGGCVTATVQEVRESRTGMDASDAV
                     VVLGRRSRPKSAETELDFVTCISRNLSKGAAGIKVIEEQQFMDSLFPWFEPRTAPVKT
                     ADLPELIALPPLARRLEEMNLRYLVWVEGSTERTDSAGSLTCSVTA"
BASE COUNT          490 a          824 c          753 g          444 t
ORIGIN      
        1 gattgctctt gccgagggcc gcgagctgga cgacgcgctg gaagctctgc gcgaggtgat
       61 acccgaccgg ggaaccagcg tcgccaacct gatcagcgcc gtcaacgagc tgaatccgcc
      121 gccggacaac gtttatctga tcatcgactc cctgccgacc cagggcaccc gcgagccgcg
      181 ccgggccacc atctccggcg gcgatcggct gaagctgttc gcagacgcca ttcagacact
      241 tcccaagcag gtgcccatca acgtgatcat gtttcccatg gagggagatc ccatggcccc
      301 agccgcgtac tggaacctgg cgcgcatatc cggcggggcc ttcctctcgc catcacgcga
      361 ctggccatga agacccgcaa ccgaagagcc atcgaagagt tcagcgtctc gtttctcgac
      421 gtgatctgct gcggtttcgg cgccatcatc ctgctcctga tgatcaccaa gacggttgag
      481 cccatcctgc tcgagaaatc ccaggtcaat ctcgaaggcc aggtcgccga ccgcgagcag
      541 gctcttttcg agatacaggg acagatcaag gagctgcgcc gccaggtcga cgacaccagc
      601 gggcagctgg atgacaacct gctggagctc gcacgcctcg agcagcagct caccatcatt
      661 ctaggcaagt tcgaaaccac cgcggcgcgc caggacgaca acctcacgga gacccagaaa
      721 ctggccgccg ccaagcagtc gctcaccgac gagatgcagc gtctgctcgg tgtcgacttc
      781 cgacgcagca ccgacctggt cggcggcatc gccgtcgaca gcgagtacat catcttcgta
      841 atcgacacgt cgggcagcat gtacaacgcg gcctggcccc aggttctgaa aaaagtgaac
      901 gagacccttt cgatttatcc ccgggtgaag ggcatccagg tgatgaacga catgggggat
      961 tacatgttcg ctcgcttcgc cggcgagtgg attcccgact caccgagccg gcgcaaagcc
     1021 atcctcgacc gcctgcagac gtggaacccg ttcagcaatt cctcgcccgt ggaggggatc
     1081 caggctgcca tcaacaccta ttacgatccc gacaagagaa taagcatcta cgtcttcggc
     1141 gacgatttca ccggcgaatc catcgagtcc gtcgtcgatg ccgtggaccg ggtcaacgtg
     1201 gccgacgact cggggcgccg gcgcgtgcgc attcacgccg tcgctttccc cgtatatctc
     1261 tcgcggccgt ccgggcgggt atatcgcttc gccgctctga tgcgggagct tgcctatcgc
     1321 aatgacggaa cttttgtagg gctgtcggaa tttcagtagt cttaaagggt cgaaagatca
     1381 gggacaatgg gagctgccgc gctgtgagca aacgacctcc ggcatggtcg aattcgcccc
     1441 cgctcggtag gggagcgcgg cttcgtgtcg ccctgctgac agccgtctgc atggcttttg
     1501 gtacggcctg cggaggtgcc agcgtgacgg ttcccacgaa cttccccgta cccgtgctgg
     1561 acaagctccc gctgaacatg ggcatttacc tcagcgacga cctgctcaac tacgtccacg
     1621 ccgagcgcct ggagaacgcg ggcagctggc agatcagcct gggcgacgcc cagccggcca
     1681 tgttcaacaa cctgctttca ggcatgttcc agggtgtgga gacgatctta gatccgagcg
     1741 ctccgtccgc cggcgtcaac ggcgtgctcg ttcccagcat ccaggaagtc cagttttcca
     1801 taccgtcgca gacccgcagc gattactacg aggtctggat ccgctatcaa tttcagctgt
     1861 acgacggcga agggcaactg ctggccgact ggccgctcac ggcctacgga aaagcagacg
     1921 aggacaacta cgggctctcg acggatcagc ccgccctgca ggccgcggcg ctggccgcct
     1981 gccgggacgc catggccttc ttcaccgtgc agttccgtga cgtgccgagc gtcaaagcct
     2041 ggctcgaccg ccaactggga ggcacctcat gatgggtttt catcgaatca tcgccctcgc
     2101 agcgctcgcg gctgtcgcca ccgtgctcgg gggctgcgtc accgccaccg ttcaggaggt
     2161 cagggagtcc agaacgggaa tggatgccag cgacgccgtg gtggtgctcg ggcgtcgcag
     2221 ccgacctaaa tctgccgaga cggagctcga cttcgtcacc tgcatcagca ggaacctcag
     2281 caagggggct gctggcatca aagtcatcga ggaacagcag ttcatggaca gcctgttccc
     2341 atggttcgag ccacgcacag cgcccgtgaa gacggccgat ctgccggagc tgatcgcgct
     2401 gccgcccctg gcccgacggc tggaagaaat gaacctgcgc tacctcgtct gggtggaggg
     2461 atccaccgag cggaccgact ctgccggttc gctgacgtgc agcgtgaccg c
//
LOCUS       DBNHOX010000775         7103 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1475700, whole genome shotgun sequence.
ACCESSION   DBNHOX010000775 DBNHOX010000000
VERSION     DBNHOX010000775.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1475700"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="AC16UZ_16825"
     CDS             complement(<1..1236)
                     /locus_tag="AC16UZ_16825"
                     /inference="COORDINATES: protein motif:HMM:NF012931.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HIR6248405.1"
                     /translation="MPYIAGPEGALLPLPRRFAPGPGGPGAVGVVDARFADASSARLV
                     RAVERLNDRLGRRNGEAGLKMALEISCAIPCPEHPQLGQPEAYQLNVTDQGARLVAET
                     EWGVLHGLETLFQLATAPGGLGCVAVDDAPRFPWRGLLLDVARHYLGMDALLRTLDAM
                     AQVKLNVLHLHLTDDQAFRFPSERFPELASPRHYSRRELAELINHAADRGIRVVPEID
                     VPGHTTCWLVARPDWGTGVTTSTRRFGVHEACLDPTSEAVYAALAELLGEVAEVFPDR
                     HLHLGGDEVHPAWWSASDRVQTFMSERGMTGLDDMQAHFNSRVARTVSGLGKRPMGWD
                     EVVHDDLPDDVVVQSWRGATARDRALGSGHDCVVSSGYYLDLFYPADVHYRFDPQAPE
                     SELVGLEDALLDDPRFEHVA"
     gene            1370..1978
                     /locus_tag="AC16UZ_16830"
     CDS             1370..1978
                     /locus_tag="AC16UZ_16830"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HIR6248406.1"
                     /translation="MKIQERMRAVLQAEAEAIAAVRIDETFEHAVRALLECRGKVITT
                     GIGKAGHIARKFAATLCSGATPADFIHPAEAAHGDLGLVGPDDVMIAFSTSGKSREVL
                     EILELARHLGLAMVIGITSHPDSELRDLSDLVLDMGEIEEPCPVGLTPSASTAVMLAI
                     SDAIALALMEAKGITKEDWGLRHHGGYLGRAARADNVPHDGG"
     gene            complement(1989..2870)
                     /locus_tag="AC16UZ_16835"
     CDS             complement(1989..2870)
                     /locus_tag="AC16UZ_16835"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6248407.1"
                     /translation="MQIERHEIRIFSAVVEEGGFSRAAERLNITQSAVSQAVANLEHK
                     LDARLLERRGKPELTEAGKRLFAFTQAVIKEETNALDDIAQIRAGALSTLNLGMSSMF
                     NRLFGRELLLEFCERNPLTRLKLDVAPSRELIYGVDEDRWELGIGPFQTLMPGHFETK
                     RFFEEERVLVAHEKHPRFDALMSQPARELAQLTLLTSYLDDATKRPGSERLRNQFASV
                     WEISSLELRLTLAEAGMGVTYVSDRLVGDLEGFHPIDHMEISTIPRVVGIFHKKHVAL
                     SEGAKRFLAICQRRFAG"
     gene            2969..4129
                     /locus_tag="AC16UZ_16840"
     CDS             2969..4129
                     /locus_tag="AC16UZ_16840"
                     /inference="COORDINATES: protein motif:HMM:NF012506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR6248408.1"
                     /translation="MVADIIAALRCGPFANDKGANDMSSDTLPKGLEGVEVDETGISL
                     VDGERGVLSYRGIDVDELVDKPFDEVAALVATGRLDPDFGARLAASAELSEREAGLVR
                     SLPRDVHPMHVVQGLTPLLDRDGAFADFGDAAQGFAVAAKLPALIATHMLGTAVAGYP
                     TLGHAERFLAQIAAPQTDIARRAFNVTQILQIEHGFNASTFAARVAASTLAPVENALS
                     AGFGTLHGRLHGGADQAALEVAEQVGTAAAAAAFVDRCIADGTRVMGMGHREYRVLDP
                     RARHIKELARSLAAGTPHEKTFETLAAIEDRFRERMAEKGKALHANVEFYKGVVYRVL
                     GMTPAFFTTGFAMARVFGYLAHFIESREDNRLIRPKARYTGPPVGAVRLTPA"
     gene            complement(4116..4529)
                     /locus_tag="AC16UZ_16845"
     CDS             complement(4116..4529)
                     /locus_tag="AC16UZ_16845"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6248409.1"
                     /translation="MSGRSNDGGGREFRVTDVRVYRPYPDEVPWDLLDRQAPADPAGD
                     GVQMRIAKLGDHVVGAYVIEPVDPLTFRIVDLVVAEGWRRRGIGRWLLGHAIGLAESR
                     GAREVRVTNPSPRRFFASIGFLDADAGMRFPITPE"
     gene            complement(4548..4976)
                     /locus_tag="AC16UZ_16850"
     CDS             complement(4548..4976)
                     /locus_tag="AC16UZ_16850"
                     /inference="COORDINATES: protein motif:HMM:NF051508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7832 domain-containing protein"
                     /protein_id="HIR6248410.1"
                     /translation="MIYDDLDRHRPAAGAIERAVVPMGMYLAWCVNLQLTGRALEEEA
                     GTLLLRVRYREKTGSELLLAACGGVLDGRWLNDAGRRFTDGYYGGYLDDFRSVFGSDV
                     YGVEDDWDHYDRLAPLLTRRYLGESAKTDRGARRWWKFWQ"
     gene            complement(4976..6139)
                     /locus_tag="AC16UZ_16855"
     CDS             complement(4976..6139)
                     /locus_tag="AC16UZ_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR6248411.1"
                     /translation="MNWPKDRYGFETRAIHAGQAPDPATGAVVTPVYLTSTFQHSEPG
                     EFAGFDYSRAGNPTRKAFEDCVANLEGARFGFAFASGCIGAATVVHLLEQGDHVVACD
                     DMYGGTHRLFEQVFRPQGIDFSYLDLSSADRLRGALRPETRMVWIESPTNPLMKLVDI
                     AAVATIAREHGAMLMVDNTFMTPYLQRPLELGADMVLHSTTKYINGHSDLIGGLVVTD
                     DEQLAERLDFLNKTMGGIQGAFDSYLCLRSVKTLAVRMQAHERNARAVAEMLASHPKV
                     EWVGYPGLSSHPQHELARRQASGFGGMVAFRVTGGLAAARRLLGAVEVFTLAESLGGV
                     ESLIEHPGLMTHASLPADRRAALGIDDGLIRVSVGIETTGDLLADLEAALAAA"
     gene            complement(6221..>7103)
                     /locus_tag="AC16UZ_16860"
     CDS             complement(6221..>7103)
                     /locus_tag="AC16UZ_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248412.1"
                     /translation="ATIEKVAVAAVMAGCLPDYMPLVVAAVRAVIDPVFDLTEMQGTT
                     HCTAPLIIVNGPARHWCGPIASGFGALGPGHRANASIGRALRLTMINIGGARPGVSDM
                     ALLGHPGKFTYCLAEDEEESPFTPLHVSRGFAAEDSVVTVIGAEAPHSVLYSVDADDP
                     EDHEKLLGILAVGLGNLATNNAQLRNGSAVVVLNPDHAAVLANAGLSREDVAARLQEL
                     AVHSRADIERYGKAFMNWSRDEEVFHCFQDPAQILVVMAGGSGLYSMVMPSWCAGGHR
                     NSPVSVRVELDQACEIP"
BASE COUNT         1215 a         2429 c         2355 g         1104 t
ORIGIN      
        1 ggccacgtgc tcgaaccggg ggtcgtcgag gagcgcatcc tcgagcccga cgagctcgga
       61 ctccggggct tgcggatcga agcggtagtg cacgtcggcc ggatagaaga ggtccaggta
      121 gtagcccgac gaaacgacgc agtcgtggcc gctacccaga gcccggtccc gcgcggtcgc
      181 gccccgccag ctctgaacga ccacgtcgtc gggcagatcg tcgtgcacca cctcgtccca
      241 acccatgggc cgcttgccaa gcccactcac cgtgcgggcc acccgggagt tgaagtgggc
      301 ctgcatgtcg tcgagcccgg tcatgccgcg ctccgacatg aaggtctgaa cgcggtcgct
      361 tgccgaccac caagccggat gcacctcatc gccccccaga tgcagatggc gatccggaaa
      421 cacctcggcc acctcgccca gcagctcggc cagagcggcg tagacggctt cgctggtggg
      481 atccaggcag gcctcgtgga caccgaaccg cctggtcgac gtcgtcacgc cggtccccca
      541 gtcggggcgg gccacgagcc agcaggtcgt gtgcccgggg acgtcgattt cgggcaccac
      601 gcgaatgccg cggtcggccg cgtggttgat gagctcggcg agctctcgcc gggaatagtg
      661 tcgtgggctg gcgagctcgg ggaatcgctc gctgggaaac cggaacgcct ggtcgtccgt
      721 caggtgcaga tgcaggacgt tcagcttcac ctgagccatg gcgtcgagcg tacgcaggag
      781 cgcgtccatg ccgagatagt gccgggccac gtccaggagc agaccgcgcc agggaaatct
      841 gggcgcatcg tcgacagcca cgcagccgag gccgcccggc gccgtcgcca gctggaacag
      901 ggtttccagc ccgtgcagga ccccccactc ggtctccgcc acgagccggg cgccctggtc
      961 agtcacgttg agctggtaag cctccggctg gcccagctga gggtgctccg gacacgggat
     1021 cgcgcaggag atctcgaggg ccattttcag acccgcctcc ccgttgcgcc ggcccagacg
     1081 gtcattcagc cgttccaccg cgcgtaccag ccgcgccgag ctggcgtcgg caaagcgggc
     1141 atccacgacg cccaccgccc ccggcccacc aggccccggg gcaaaccggc gcggcagggg
     1201 taacagcgcc ccttccggtc ccgctatgta cggcaagcgt cgtgcctcga acgtcgaaga
     1261 gacccggact ctgtaagagg cgccagacgc tgtcaatgca cggcgtcacc ttgaggacgc
     1321 cggatgcgcg tgacaccatt gcgcggggca atcgcggacc gtgtcggcaa tgaaaattca
     1381 ggaacgtatg cgggccgtgc tgcaggccga ggccgaggcc attgcggcgg ttcggatcga
     1441 cgagacgttt gagcacgccg tccgcgccct gctcgagtgc cgcggcaagg tgatcaccac
     1501 gggcatcggc aaggcgggtc acatcgcccg caagtttgcc gccacgctct gctccggcgc
     1561 gacgccggca gacttcatcc accctgccga agccgctcac ggggacctgg ggctggtggg
     1621 acccgacgac gtgatgatcg ccttctcgac cagcggcaag agccgcgagg tgctggaaat
     1681 actcgagctg gccaggcacc tgggcctggc catggtcatc ggcatcacct cccaccccga
     1741 ctccgagcta cgcgaccttt ccgacctggt gctggacatg ggcgagatcg aagaaccttg
     1801 cccggtgggg ctgaccccca gcgccagcac ggccgtcatg ctcgccatca gcgacgccat
     1861 tgcgctggcg ctcatggagg ccaagggcat caccaaggaa gactggggct tgcgccatca
     1921 cggcggctat ctgggcaggg cggcccgcgc cgacaacgtg ccccacgacg gcggctgacc
     1981 cgctcgaatc agccggcaaa ccgccgctgg cagatggcca gaaagcgctt ggcgccttcc
     2041 gaaagggcaa cgtgtttctt gtggaagatg cccaccactc ggggaatggt cgagatttcc
     2101 atgtgatcta tgggatggaa cccctccagg tcgccgacca gccggtcgga aacgtaggtc
     2161 acgcccatgc ccgcctccgc cagagtcagg cgcagctcca gactgctgat ctcccagacg
     2221 ctcgcaaact ggtttctaag gcgctcgctg cccgggcgct tggtggcgtc gtccagatac
     2281 gaggtgagca gggtcagctg agccagctcg cgggcgggtt gactcatgag cgcgtcgaag
     2341 cgcgggtgct tctcgtgcgc caccagcacg cgctcctcct cgaagaatcg tttggtctcg
     2401 aagtgtccgg gcatcagggt ctggaacggc ccgatgccga gctcccagcg atcctcgtcg
     2461 acgccgtaga tgagctcgcg gctcggcgcc acgtcgagct tgagccgggt cagcgggttg
     2521 cgctcacaga actcgagcag cagctcgcgg ccgaacagcc ggttgaacat gctgctcatc
     2581 cccagattga gggtcgaaag cgcgccggcg cgaatctgcg cgatgtcgtc cagcgcgttg
     2641 gtttcctctt tgatcacggc ctgggtgaac gcgaacagcc gcttgcccgc ctccgtgagc
     2701 tccggcttgc cgcggcgttc gagcagccgg gcatcgagct tgtgctcgag attcgccacc
     2761 gcctggctga ccgccgactg ggtgatgtta agacgctcgg cggccctgct gaagccgcct
     2821 tcctccacca cggcgctgaa gatcctgatt tcgtggcgct cgatctgcat gtccggaggc
     2881 taccagggcc tgcaggggcg ctccacttat agagccatta tctgggctaa tggataagcc
     2941 ggcctgatct ccggagaagt gaaagcggat ggtcgccgac atcatcgccg cgctacgctg
     3001 cgggccgttc gcgaatgaca aaggagccaa cgacatgtcg agcgacaccc tgcccaaagg
     3061 gctcgaaggc gtggaggtgg acgagaccgg gatctcgctg gtggacggtg agcgcggggt
     3121 cctgagctac cgaggcatcg acgtggacga gctggtcgac aagcctttcg acgaggtcgc
     3181 ggcgctcgtg gcgacgggcc ggctcgatcc cgatttcggc gcgcgcctgg cggcctcggc
     3241 ggagctgtcc gagcgcgagg cggggctggt ccgctcgctg ccgcgcgacg ttcatcccat
     3301 gcacgtagtg cagggactca cgccgcttct tgaccgcgac ggggcctttg ccgacttcgg
     3361 cgacgccgct cagggatttg ccgtggcggc caagcttcct gcgctgatcg cgacgcacat
     3421 gctcggcacc gcggtggcgg gctatccgac attggggcac gcggagcggt tcctggccca
     3481 gatcgcggcc ccgcagaccg acatcgcccg gcgcgccttc aacgtcaccc agatcctgca
     3541 gatcgagcac ggcttcaacg ccagcacctt tgccgcccgg gtcgcggcga gcaccctcgc
     3601 accggtggaa aatgccctgt ccgccggctt cgggaccctc cacgggcgtc tgcacggcgg
     3661 cgcggaccag gcggcgttgg aggtcgccga gcaggtgggc acagcggcgg ctgcggcggc
     3721 gttcgtggac cgatgcatcg ccgacgggac ccgcgtgatg ggcatggggc atcgggaata
     3781 ccgcgtgctg gacccacgcg cgcgccacat caaggaactc gcgcgcagcc tcgccgccgg
     3841 cacgccccac gagaagacct tcgagacctt agcggccatc gaggatcggt ttcgcgagcg
     3901 gatggccgag aagggcaaag cgctccacgc caacgtggag ttctacaagg gcgtcgtcta
     3961 ccgggtgctt ggcatgacgc ccgcgttctt caccacgggc tttgccatgg cccgggtgtt
     4021 cggctacctg gcccacttca tcgagagccg cgaggacaac cgcctgatcc gcccgaaagc
     4081 tcggtacacg ggcccgcccg tgggcgcagt gaggctcact ccggcgtgat gggaaatcgc
     4141 atccctgcat cggcgtccaa gaaaccgatg ctggcgaaga accgccgagg ggatggattg
     4201 gtcacccgaa cctcgcgagc cccacgggac tccgccagcc cgatggcgtg acccagaagc
     4261 catctgccga tcccgcgccg gcgccagcct tcggccacca ccaggtccac gatcctgaag
     4321 gtcagcggat cgacgggttc gatgacgtaa gcgcccacca cgtggtcgcc gagcttcgcg
     4381 atgcgcatct gcaccccgtc tcccgcggga tccgccggtg cctgccgatc cagcaggtcc
     4441 cagggaacct cgtctggata aggccggtaa acgcgcacgt cggttacccg gaattcccgt
     4501 ccgccaccgt cattagacct accgctcatt gccagctcat cgccaaatca ttgccagaac
     4561 ttccaccatc gccgggcgcc gcgatccgtc ttagcgctct ctccgaggta ccggcgcgtc
     4621 agcaggggcg caagacgatc gtagtgatcc cagtcgtcct ctacgccgta gacgtcggat
     4681 ccgaaaaccg agcggaagtc gtccagatat ccaccgtagt agccgtctgt gaagcgacgg
     4741 cccgcgtcgt tcagccagcg gccgtccagc acgccgccgc aggcggcgag cagcagctcg
     4801 ctaccggttt tctcccggta gcgaacccgc agcaaaaggg tcccggcctc ctcctccagc
     4861 gcgcggcccg tcagctgaag attgacgcac caggccaggt acatgcccat gggcaccacg
     4921 gcgcgctcaa tggcgccggc agcgggccga tgccggtcca gatcgtcgta aatcactagg
     4981 cggcagccag cgcagcttcg agatcggcca ggagatcgcc cgtcgtctcg atccccacgc
     5041 tgacgcggat gagcccgtcg tcgatcccga gcgcggctcg gcgatcggcc ggcaggctcg
     5101 cgtgggtcat caggcccgga tgctcgatga gggactcgac gccacccagg ctctcggcca
     5161 gggtgaacac ctccaccgcg ccgagcaggc gccgggcagc agccaggcca cctgtgacgc
     5221 ggaaggcgac catcccgccg aagcccgagg cctgccgccg agccagctcg tgctgcggat
     5281 ggctcgacag cccagggtag cccacccatt ccaccttcgg gtgcgacgcc agcatctccg
     5341 caaccgcccg cgcgtttcgc tcatgtgcct gcatgcgaac ggccagggtc ttcacgctgc
     5401 gcaggcaaag gtagctgtcg aacgcgccct ggatgccgcc catggtcttg ttgagaaagt
     5461 cgaggcgttc ggcaagctgc tcgtcgtcgg tgaccaccag cccgccgatc aggtcgctgt
     5521 gaccgttgat gtacttcgtg gtggagtgca gcaccatgtc ggccccgagc tccagcgggc
     5581 gctggagata aggggtcatg aaggtgttgt ccaccatcag catcgcgccg tgttcgcggg
     5641 caatcgtcgc aacggcggca atgtccacga gcttcatcag aggattggtg ggcgactcga
     5701 tccagaccat gcgggtctcc ggtcgaagcg ccccgcgaag ccgatccgcc gagcttagat
     5761 cgaggtagct gaagtcgatc ccctggggcc ggaatacctg ctcgaacagg cggtgggtgc
     5821 cgccatacat gtcgtcacag gccaccacgt gatcgccctg ctcgagcagg tgaacgaccg
     5881 tcgccgcgcc gatgcagcca gacgcaaagg cgaagccgaa ccgcgcccct tccaggttgg
     5941 caacgcagtc ctcgaaggcc ttgcgggtcg ggttgccggc ccgggagtaa tcgaagccgg
     6001 caaactcgcc tggctcggag tgctgaaagg tcgaggtcag gtaaacgggg gtcaccacgg
     6061 cgcccgtcgc ggggtcgggg gcctgccccg cgtgaatcgc ccgggtctcg aatccatatc
     6121 tgtctttggg ccagttcatg ctgcagctcc ctgaaacctt tgcctccggc gacccatcgc
     6181 ccccggccgg agcacctccg acggcatggc tcgcggcagg tcaggggatc tcgcaggcct
     6241 ggtcgagttc gaccctgacg ctgaccggag agttcctgtg gccgcccgcg caccaggagg
     6301 gcatgaccat ggaatacagc ccgctgccgc cggccatcac cacgaggatc tgcgcggggt
     6361 cctgaaagca gtgaaatact tcttcgtccc tggaccagtt catgaacgcc ttgccgtagc
     6421 gctcgatatc ggcacgggag tgcacggcca gctcctgcag gcgggcggcc acgtcctcgc
     6481 gactcaagcc cgcattggcg agcacggcag catgatccgg gtttagcacc accacggcgg
     6541 agccgttgcg cagttgggcg ttgttcgtag ccaggttgcc caggcccacg gcgaggatgc
     6601 cgagcagctt ctcgtgatcc tcgggatcgt cggcatcgac gctgtaaagc acggagtgcg
     6661 gcgcctccgc gccgatgacg gtcaccacgc tgtcttcggc cgcgaagccc cgggaaacgt
     6721 gaaggggggt aaacgggctc tcctcctcgt cctcggcaag acagtaggtg aactttccgg
     6781 gatggccgag cagggccatg tcggagacgc cagggcgggc gccgccgatg ttgatcatcg
     6841 tgagccgcag cgctcgcccg atggaggcgt tggcccgatg ccccggtccc agggcgccga
     6901 agccgctggc gatgggaccg caccagtgcc gcgcgggccc gttgacgatg atcaggggcg
     6961 ccgtgcagtg ggtggtcccc tgcatctccg tgaggtcgaa gacgggatcg atgaccgccc
     7021 gcacggcggc caccaccaac ggcatgtagt ccgggaggca gccggccatc accgctgcca
     7081 ccgccacctt ctcgatagtg gcg
//
LOCUS       DBNHOX010000776         1806 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_62614, whole genome shotgun sequence.
ACCESSION   DBNHOX010000776 DBNHOX010000000
VERSION     DBNHOX010000776.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_62614"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="AC16UZ_16865"
     CDS             <1..879
                     /locus_tag="AC16UZ_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248413.1"
                     /translation="YVEGSARLDGVPVEPAMGERDLAWGGLTLAPDSQRSIKMLLGVS
                     AGVSEGEFVNRAQAINTVGQINLSGEATATVRVVPDPTFDCTDVTGKVYDDGNRNGRQ
                     DLDEFGLQGVRLVTPQGLVATTDEHGRFHITCATVPRDDRGSNFALKLDDRTLPSGFR
                     ASTDRVQIKRATRGKALHFEFGASIHRVVGLEIADGVFFPESSEIRPQWKHRFELLLE
                     ELTKSPSVLRLTYLADVEDPKLVKQRLRVVKKQIVRSWRELDCCYRLELEDDVFWRLG
                     APAGKAVNRRVDGGDE"
     gene            872..>1806
                     /locus_tag="AC16UZ_16870"
     CDS             872..>1806
                     /locus_tag="AC16UZ_16870"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR6248414.1"
                     /translation="MSKQYPIIPMWLARWLACAVAILCAPAAAEKVQETTATHAAERH
                     LSSDQTFTQWAFDPDRLAQADGDRVEKIAVATVEPRTVKLKDVVRPIYFDSGVAEIPA
                     STVAELRHVLESMRERRNVRLNLVGHADSESLSPELEDVYGDNFGLSRERAGQVADYL
                     QAVLALPAEALSIEWAGDTRPVASNDTEAGRALNRRVEVEVWYDELDDGTAIEERVVI
                     DEIERVKVCRVETVCKLRYLDGHDRRARVLNLITPLHYGEEGVEVTDEFTAQVQRTMA
                     SLGDRQNVTVKFIGYTDNQTLAGRTARIYGTHEVLS"
BASE COUNT          320 a          594 c          621 g          271 t
ORIGIN      
        1 tacgtggagg gctcggcgcg cctggacggg gtgccggtgg agccggcgat gggcgagcgc
       61 gatctcgcct gggggggcct caccctcgct ccggacagcc agcgcagcat caagatgctg
      121 ctcggagtca gcgcgggtgt cagcgaaggc gagttcgtca accgcgccca ggccatcaat
      181 acggtgggcc agatcaatct ttccggcgaa gccaccgcta ccgtgcgggt ggtgcccgat
      241 cccaccttcg actgcacgga cgtcaccggc aaggtttacg acgacgggaa ccgcaacggg
      301 cgtcaggacc ttgacgagtt cggcctgcag ggcgttcgcc tggtcacccc ccagggcctg
      361 gtcgccacga cggacgagca cggtcggttc cacatcacgt gcgccaccgt gccgcgcgac
      421 gaccgcggca gcaacttcgc gctgaagctg gacgaccgga ccctgccaag cggtttccgc
      481 gcctccaccg accgggtgca gatcaagcgc gccacccggg gtaaggccct tcacttcgag
      541 ttcggcgctt cgattcaccg tgtggtgggt ctggagatcg ccgacggcgt gttcttcccg
      601 gaaagcagcg agatccgtcc tcagtggaag caccgcttcg agctcctgct cgaggagctc
      661 accaagtcgc cctcggtgct gcggctgacc tacctggccg acgtcgagga tccgaagctg
      721 gtcaagcagc gcctgcgcgt ggtgaagaag cagatcgtgc gcagctggcg ggagctcgac
      781 tgctgctacc ggctcgagct cgaggacgac gtgttctggc ggctgggcgc gcccgcgggc
      841 aaggccgtga atcgacgggt ggatggcggc gatgagtaag cagtacccaa tcatcccgat
      901 gtggctcgcc cgttggctgg cgtgcgccgt ggccatcctc tgcgcgcccg cggcggccga
      961 gaaggtgcag gaaaccaccg cgacccacgc agccgagcgg catctctcca gcgaccagac
     1021 gttcacccag tgggctttcg atccggaccg actggcccag gccgatgggg accgggtcga
     1081 gaagatcgcg gttgcaaccg tcgagccgcg caccgtcaag ctcaaagacg tcgtccgccc
     1141 gatttatttc gactccggcg tcgcggagat tccggccagc accgtcgccg agctgcgcca
     1201 tgtgctggaa agcatgcgcg agcggcgcaa cgtacggctg aatctggtgg gccacgcgga
     1261 cagcgagtcg ctttccccgg agctcgagga cgtctacggc gacaacttcg ggctgtcccg
     1321 ggagcgagcg ggccaggttg ccgactatct gcaggcggtg ctcgcgctgc ccgccgaggc
     1381 gctgtccatc gagtgggccg gcgacacccg gcccgtggcc tccaacgaca ccgaagcagg
     1441 ccgcgcgctc aaccgccgcg tggaggtcga ggtgtggtac gacgagctcg acgacggcac
     1501 tgccatcgaa gagagagtgg tcatcgacga gattgagcgc gtgaaggtgt gtcgcgtcga
     1561 aacggtgtgc aagctccgct atctcgatgg acacgaccga cgcgcccggg tgctgaacct
     1621 gatcacgccg ctgcactacg gcgaggaagg cgtggaggtc accgacgagt tcacagccca
     1681 ggtgcaacgc accatggcga gcctgggcga ccgccagaac gtcacggtga agttcatcgg
     1741 ctacacggac aaccagacgc tggcaggccg cacggcgaga atctacggga cccacgaagt
     1801 gctctc
//
LOCUS       DBNHOX010000777         5084 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1028005, whole genome shotgun sequence.
ACCESSION   DBNHOX010000777 DBNHOX010000000
VERSION     DBNHOX010000777.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1028005"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..686)
                     /gene="pgsA"
                     /locus_tag="AC16UZ_16875"
     CDS             complement(87..686)
                     /gene="pgsA"
                     /locus_tag="AC16UZ_16875"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012315213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR6248415.1"
                     /translation="MNLPNLLTLGRILFVPVFVLVYVIPGERTYLLAATLFALAAFTD
                     WLDGYLARRLNQTTPFGAFLDPVADKLIVVTALVLLIGHHRTLWLTLPALVIVGREIV
                     ISALREWMAEMNRRGVVAVSWLGRVKTTFQMIAVVVLLANPPAFDRPWVLLGYGLLYV
                     AAAMTLASMALYLHAAWPTLKSGYGGPEGEPGEDPRDRS"
     gene            complement(723..2540)
                     /gene="uvrC"
                     /locus_tag="AC16UZ_16880"
     CDS             complement(723..2540)
                     /gene="uvrC"
                     /locus_tag="AC16UZ_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019396703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR6248416.1"
                     /translation="MTAFDSEAFLGTLTRKPGVYRMLDADGNVLYVGKARNLRSRVSS
                     YFRASGLATKTMALVGRIADIQITLTNSETEALLLEQSMIKEERPPYNIVLRDDKSYP
                     FIYLTEGEPFPRLTFHRGAKRRKGRYFGPFPSASAVRDSLNILQKLFQLRHCEDSFFK
                     NRSRPCLQYQIRRCSGPCVGLVDSQTYAEDVKLAVMFLEGRSNAVLEAFKGNMQKAAE
                     AMDYEAAAKYRDQIAHLRKVQEQQYVHAAEGDVDVFALMEASGVCCVQALFVRAGRML
                     GQRTWFPKNELGLEPAEMLSAFLSQYFLAGAERDVPRTVITAERIVDQDLLADALSKR
                     SGRRVDVLSQVRGQRARWVRLGLDNAAHSLNAYLADKRNVYARLVALQDLLGLEDVPE
                     RLECFDISHTMGEATVASCVVFDRNGPLKSDYRRFNIEDVAAGDDYAAMEQALRRRYT
                     RLKQGEGKLPDVLVIDGGRGQLQRAKDVLDELQVDGVHLLAIAKGPSRKPGLEKLFAN
                     GAEVDYPEQGAAAHLLQHIRDEAHRFAITGHRQRRQRKRSRSELDDVAGVGPKRRREL
                     LTHFGGLSGVKGASVEEIAKVPGISRRLAEEIYGTLHAE"
     gene            complement(2577..3215)
                     /locus_tag="AC16UZ_16885"
     CDS             complement(2577..3215)
                     /locus_tag="AC16UZ_16885"
                     /inference="COORDINATES: protein motif:HMM:NF015146.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HIR6248417.1"
                     /translation="MRYDRLESCRICPRLAAHLDQVRQAHPGYRCLPVGATGSVRAKL
                     LMVGLAPGLHGANRTGRPFTGDASGTFLFAALARVGLASDADPLVARLVGARLTNAVK
                     CLPPQNRPNAAEMSACSRYLREEIDELWSPQVRSARCVLCLGRLAHRSVEIALGKRLP
                     AFLHGQLCEVKPGLWLADSYHPSRQNTNTRRLKPEMLDSVLENVKGVVGLTG"
     gene            complement(3205..3867)
                     /gene="uvrY"
                     /locus_tag="AC16UZ_16890"
     CDS             complement(3205..3867)
                     /gene="uvrY"
                     /locus_tag="AC16UZ_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HIR6248418.1"
                     /translation="MIRVMVVDDHKLFRLGLRRMLADVKGIHVIGEADCGEAAVRLAR
                     AERPNIVLMDILMPGIGGIEATRRIARLEMDIKVIAVTGCHENPFPMQMLRAGACGYL
                     SKGVDRDELVAAIRKVFLGRRYLSADIAQEVAASAFDQTRESPFEQLSNREMQIMMMV
                     VNCHKVNDISEDLHLSPKTVNSYRYRIFEKLNVSSDVELALMAVRHGMVIPQLNGMAR
                     AV"
     gene            complement(4007..>5084)
                     /locus_tag="AC16UZ_16895"
     CDS             complement(4007..>5084)
                     /locus_tag="AC16UZ_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR6248419.1"
                     /translation="QRELMPRVLKANREEHFDREIMNEMGALGLLGATLPAKYGCSAV
                     NYVSYGLMAREVECVDSGYRSAMSVQSSLVMHPIYAYGNEDQRERYLPKLASGEWVGC
                     FGLTEPDHGSDPGGMVTRAEPVSGGYRLNGAKMWITNSPIADLAVVWAKLDGVIRGFV
                     VERAFDGFSTPKIEGKMSLRASVTGEIVLDDVVVPEENLLPNVKGLAGPFGCLNRARY
                     GIAWGSMGAAEFCWHAARTYTLDREQFGRPLAANQLIQKKLADMQTEIALGLQGCLRM
                     GRLLDEDRLAVENISMLKRNNCGKALEVARMARDMHGGNGISDEYHVIRHVMNLETVN
                     TYEGTHDIHALILGRAQTGLQAFS"
BASE COUNT          932 a         1669 c         1565 g          918 t
ORIGIN      
        1 actctgccac tcagccttcg tcgccggccg gcgcgcgacg tcagtcgcgg tcgccctgcg
       61 aacacgccgg cgaattctag cgggctttag gagcgatcgc gagggtcttc gccgggctcg
      121 ccttccgggc cgccgtaacc ggacttcagc gtcggccagg cagcatgcag atacagggcc
      181 atggaggcga gggtcatggc ggcggcaacg taaaggagcc catagccgag cagcacccag
      241 ggccgatcga aagccggcgg gttggccagc agcacgacca ccgcgatcat ctgaaaggtg
      301 gttttcaccc tccccagcca ggaaaccgca acgacgccgc ggcggttcat ttccgccatc
      361 cactcgcgca gcgcgctgat gacgatctct cgcccgacga tcaccagcgc agggagggtc
      421 aaccacagcg tgcggtggtg gccgatgaga agcacgagag cggtgaccac gatgagcttg
      481 tccgccaccg ggtcgagaaa cgccccgaag ggcgtggtct ggttgaggcg tcgggccagg
      541 taaccgtcca gccagtcggt gaatgcggcc agcgcaaaca gcgtggctgc cagcaggtaa
      601 gtacgttctc ccgggatgac gtagacgagc acgaaaaccg gcacgaaaag aattcgcccc
      661 agcgtgagca gattgggtaa gttgatgacc ggtttcatgg ctgtgtgctc accgcgtaac
      721 gatcactccg cgtgcagggt accgtatatc tcttccgcca gcctgcggct gattccgggc
      781 accttggcga tctcctcgac gctggcacct ttcacgccgg agagcccacc gaagtgggtc
      841 aacagctcgc gccgccgctt cggccccacg ccggcgacgt catcgagctc cgatcggctg
      901 cgtttgcgct gacggcgttg gcggtggccg gtgatggcaa agcggtgcgc ttcgtcgcga
      961 atgtgctgaa gcagatgagc ggcagccccc tgctctgggt agtccacctc cgcgccgtta
     1021 gcaaacagct tttccagtcc cggctttctg ctgggccctt tggcgatggc cagcaggtgc
     1081 acgccgtcga cctgcaactc gtcgagaacg tccttagccc gctgcagttg cccgcggcca
     1141 ccgtcgatca ccagcacgtc gggcagcttt ccttcgccct gcttcaagcg agtgtatcga
     1201 cgccggagcg cttgctccat agctgcgtag tcatcgccgg ccgccacgtc ctcgatgttg
     1261 aatcgccgat agtccgactt gagcggcccg ttgcggtcga agacgacgca ggacgcgacc
     1321 gtggcctcgc ccatggtgtg gctgatgtcg aagcactcca ggcgttccgg cacgtcctcc
     1381 agaccaagca ggtcctgaag ggcaaccagg cgggcataaa cgttgcgctt gtcggccaga
     1441 taggcgttca ggctgtgggc cgcgttgtcg agacccagcc tcacccagcg ggcgcgctgg
     1501 ccgcgaacct gggagaggac gtccacccgg cgcccggacc gcttggaaag cgcgtcggcg
     1561 agaagatcct ggtcgacgat gcgctccgcg gtgatcaccg tgcgcggcac gtcccgttca
     1621 gcgccggcca gaaagtactg agacagaaaa gcgctcagca tctcggccgg ctccagaccg
     1681 agctcgttct ttggaaacca cgtcctctgc cccagcatgc gccccgcgcg cacgaacagc
     1741 gcctgcacgc agcacacgcc ggaagcctcc atcagggcga agacgtccac gtctccttcc
     1801 gcggcgtgaa cgtactgctg ctcctgaacc ttgcgcaggt gggcgatctg atcccggtac
     1861 tttgccgcgg cttcgtagtc cattgcttcc gccgctttct gcatgttccc cttgaaggcc
     1921 tctagcaccg cgttgcttcg accctccaga aacatcaccg caagcttcac gtcttcagca
     1981 tacgtctggg aatcgaccag gcccacgcag ggaccgctgc agcgtcggat ctggtactgc
     2041 aggcacggtc gcgaccggtt cttgaagaac gaatcctcgc aatgacgcag ctgaaagagc
     2101 ttctgcagaa tgttcaggct gtcccgcacg gcgctcgcgg aaggaaacgg cccgaagtag
     2161 cgacctttgc gtcgttttgc gccccggtga aacgtcagcc gagggaaggg ctcaccttcg
     2221 gtgagataaa tgaacgggta agatttgtcg tcacgaagga cgatgttgta cggcggccgc
     2281 tcctccttga tcatgctctg ctcgagcaac agcgcttccg tctcgctgtt ggtgagcgtg
     2341 atctggatgt ccgcgatgcg gccgaccaga gccatggtct tggtcgcgag tcccgaagca
     2401 cggaaatagc tgctgacccg gctcctgaga ttccgggcct tgcccacgta cagcacgttt
     2461 ccgtcggcgt cgagcatgcg ataaacgccg ggtttgcgcg tcagggtccc aaggaaagct
     2521 tcggagtcga aggccgtcat cggcggaact atagagtccg cgcccatggg ccgcaatcag
     2581 ccggtcaacc cgacgacgcc tttcacgttt tccaggaccg aatcgagcat ctcgggtttg
     2641 agccgacgcg tgttcgtgtt ctgacggctt ggatgatagg agtcggccag ccacaggccc
     2701 ggcttcacct cgcagagctg cccatgaagg aatgcgggca gacgctttcc gagagcgatc
     2761 tccaccgagc gatgggccag gcgcccaagg cacagcacgc agcgcgccga tcgcacctgc
     2821 ggagaccaca gctcgtcgat ctcctcgcgc aggtagcggc tacaggctga catctccgcg
     2881 gcattgggcc ggttctgcgg cggcaggcat ttgacggcgt tcgtcaaccg ggccccgacg
     2941 agacgagcaa cgagaggatc agcgtcactg gccaatccga ccctggcaag cgccgcgaaa
     3001 agaaacgtgc cgctggcgtc ccctgtaaag gggcgtcccg tgcggttggc gccgtgcagc
     3061 cccggcgcga gcccgaccat cagcagcttg gcgcgcacag accccgtggc gcccacgggc
     3121 aggcagcggt aaccgggatg ggcttgccgt acctgatcga gatgagcggc caaccgaggg
     3181 cagatgcggc aactttccag gcggtcatac cgcacgggcc atcccgttca gctggggtat
     3241 caccatgccg tggcgtaccg ccatcagggc gagctccacg tcgctggaga cgttgagctt
     3301 ctcgaagatt cggtaccgat agctgttcac cgtcttcggc gaaaggtgca ggtcctcgga
     3361 gatgtcgttc accttgtgac agttgaccac catcatcatg atctgcattt cacgattgga
     3421 aagttgctcg aacgggctct cccgggtctg gtcgaacgcg cttgccgcca cctcctgagc
     3481 aatgtcggcg ctcagatagc gcctgccgag aaagaccttt cggatggccg cgaccagctc
     3541 gtcgcggtcg acgcccttgc tgagataccc gcaggctccg gcccggagca tctgcatggg
     3601 gaaagggttt tcgtggcacc cggtcacggc gatcaccttg atgtccatct ccagacgcgc
     3661 gatccgccgg gtggcttcga tgccgccgat accgggcata aggatgtcca tgagaacgat
     3721 gttcggtctt tccgcgcggg cgagccgcac cgccgcctcc ccgcagtccg cttcgccgat
     3781 tacgtgtata cctttgacgt ccgccagcat gcgccgcagc cccagacgaa acagcttgtg
     3841 atcgtccaca accataaccc tgatcatctt gcccccaaca aagtgtccag catctgaagc
     3901 agtgacgata ggcgggcgcc aggcggctaa caacggcgcg gggtgagaat ctgccgggtt
     3961 ttcacgaaat ttccattttc cgatgtcgcg ctgatatcag atacgatcag gaaaacgcct
     4021 gcagaccggt ctgggcgcgc cccagtatca gcgcgtggat gtcgtgggtc ccctcgtagg
     4081 tgttgaccgt ctccagattc atgacatgcc ggatcacgtg gtactcgtcg gagatcccgt
     4141 tgccgccgtg catgtcccgc gccatgcgcg ctacctccag cgctttgccg cagttgttgc
     4201 gcttgagcat ggagatgttc tcgacggcaa gacgatcctc gtccagcagg cgacccatcc
     4261 tgaggcagcc ctgcaggccg agggcgatct ccgtctgcat gtccgcgagc ttcttctgaa
     4321 tcagctggtt ggcggcgagc gggcgcccga actgctcccg atcgagcgtg taggtgcgtg
     4381 ccgcatgcca gcagaattcg gctgccccca tggatcccca ggcgatgccg tagcgcgcgc
     4441 ggttcaagca accgaaggga ccggccaggc ctttgacgtt gggcagcaga ttctcttcgg
     4501 gaacaacgac gtcgtcgagc acgatctccc cggtgaccga cgcccgcagg ctcatctttc
     4561 cctctatctt gggggtgctg aaaccgtcga acgcgcgctc aaccacaaac ccgcggatca
     4621 cgccgtcgag ctttgcccag accaccgcca gatcggctat tggcgagttg gtgatccaca
     4681 tcttcgcacc gttcagacgg tagccgcccg acaccggctc ggccctggtc accatgccgc
     4741 cggggtccga tccgtggtcg ggctccgtca ggccgaaaca gccgacccac tcaccgctgg
     4801 ccagcttcgg caggtagcgc tcacgctgat cctcgttgcc gtaagcgtat atcggatgca
     4861 tgaccagcga ggattgcacg ctcatggccg accggtaccc cgaatccacg cactccacct
     4921 cccgggccat caggccgtaa gagacgtagt tgacggccga acagccatac ttcgccggca
     4981 gcgtggcacc cagcagaccg agcgccccca tctcgttcat gatttcccgg tcgaagtgtt
     5041 cttcgcgatt ggccttgaga acgcgcggca tgagctcgcg ctgc
//
LOCUS       DBNHOX010000778         4382 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1156759, whole genome shotgun sequence.
ACCESSION   DBNHOX010000778 DBNHOX010000000
VERSION     DBNHOX010000778.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1156759"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1268)
                     /locus_tag="AC16UZ_16900"
     CDS             complement(66..1268)
                     /locus_tag="AC16UZ_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6248420.1"
                     /translation="MDFEFSKEDQAFREEVRAFLEEKVTPDMRQAVRKTTTVFADKEL
                     AMRWQAILVEKGWAVPAWPAEHGGTGWSVTQKYIWGEECARASAPGLIPLGLRMLAPV
                     IFKYGTEEQKNYYLPRMLSGEHYWCQGYSEPGSGSDLSSLKTSAVKDGDDYIVNGTKI
                     WTTHAQFADHIFCLVRTDTGVRPQAGITFLLIPMDTPGIKVEPIITMAGDHEVNQVFF
                     DDVRVPQANRVGPENDGWNVAKYLLEFERGGGGAAVRLKESLKELRNIASSELADGEP
                     LMDDPGFRNRISELEIRTSALEFAELASLARMSRGESPGTASSLAKNTSVEIDQGILT
                     LKLEAVQHYAMPHANKISLDGHNEPWPGPDHAETVTPRYLNVRAASVYGGSKEVQKNI
                     IAKAVLGL"
     gene            complement(1416..2006)
                     /locus_tag="AC16UZ_16905"
     CDS             complement(1416..2006)
                     /locus_tag="AC16UZ_16905"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6248421.1"
                     /translation="MSRLQKIEPEDWAPELKAAIIPENLTPLEQGLTRFFAHRPELTL
                     GLMQFGGALKTKRTLPDKLIELVRLRVAFFNQCRSCMAIRYTDALNDGVTEDLVCSLE
                     KPAEAGNLTDAEKAAIRFGELMATDHLAIDDAVYDELRKHFSESEIVELGMHVAFFVG
                     FGRLAATWHMVEELPAAFQDDSRALSPWNEESIVVR"
     gene            complement(2003..3364)
                     /locus_tag="AC16UZ_16910"
     CDS             complement(2003..3364)
                     /locus_tag="AC16UZ_16910"
                     /EC_number="5.4.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR6248422.1"
                     /translation="MTTVAEARAELAARTAGSQEISEARAEILNSGLAANLEMPHPIF
                     IESGEGPFVFDADGNRYIDTSVGFGLHILGHRHPLIEQAIKDRTEKGWMFGIHSTSQM
                     KLAELLHEASPCAERVVFCNTGSEATMYAIRAARGYSGKEKIALFDGFYHGAHDYGMW
                     LTDPTSPREAPRKLPMGRGIPRALADLTLLLPYRHEAAFDLIRRHADELALVMVEGVQ
                     SSNPQPGAGEFLCELEAVCREAGVLFAVDEVITGFRVAYGGAQERFGVRPDLATYGKI
                     LGGGLPVGAITGRADVMEVFTGLGASNGVFSGGTFSGNPLTMEAGAAAVGYLRDHPDV
                     YRGLEQAGERLAGAVNGFCQERQIPAQMKSVGSMFHMFFQREPIRSMRDVTGASVAAE
                     KAFYLHALNRGVLVPGTQRAFLSAAHSDAEIDTLIDVFQTSLADVQAEGLFSARTDKG
                     EMR"
     gene            complement(3361..>4382)
                     /locus_tag="AC16UZ_16915"
     CDS             complement(3361..>4382)
                     /locus_tag="AC16UZ_16915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6248423.1"
                     /translation="QVLHRPRGLAGSCQGEPSMAKPFRFGFQTFNAESGQSWADKARK
                     AEDLGYSSFHLADHLLGPGPALTAANHPIQNVAAIPAMAFAAAVTSTINIGCRVFCID
                     YHLPVVLIKSAMTIDMLSNGRLELGLGAGWVKSEYEAVGIDFDEPRVRIDRLADVIAG
                     VKAFRGEDLVDVHNDTLSWKDFEGLPKPVGRAPIMVGGGSPRVLRLAGREADIVSLNF
                     NNRMGMIGPDGVQMSSEQETRKKIGWIREGAGDRFDDLEIEIGAYFTVVTDDAKPVVA
                     GMAQMFGMSEDEMLRHPHGLFGSVDAICDELQRRREEFGISYFTVGEDNLEAFAPVVA
                     RLSGK"
BASE COUNT          736 a         1463 c         1392 g          791 t
ORIGIN      
        1 agaagcggcc cgggggaatg ggccccgggc gaagcgagcc agtatagccg ccgccgggcg
       61 cggggttaca gccccaggac tgccttggcg atgatgttct tctggacttc cttggacccg
      121 ccgtaaaccg acgccgcgcg cacgttcagg tagcggggcg tgaccgtctc ggcgtggtcg
      181 ggtcccggcc acggctcgtt gtggccgtcc agcgagatct tgttcgcgtg gggcatggcg
      241 taatgctgta ccgcctcgag cttcagggta aggatgccct ggtcgatttc cacggacgtg
      301 ttcttcgcca gggaggaagc ggtgccgggc gactcgcccc gggacatccg ggcaagggaa
      361 gccagctccg cgaactcgag cgccgaggtg cggatctcca gctcgctgat ccggttgcgg
      421 aagccgggat catccatcag cggctcgccg tcggccagct cgctggaggc gatgtttctc
      481 agctccttga gcgactcttt gagccggacg gccgcaccac cgccgccgcg ctcgaactcg
      541 agcagatact tggccacgtt ccagccgtcg ttctccggcc ctacccgatt cgcctggggc
      601 actcgcacgt cgtcgaagaa cacctggttg acctcgtggt cgccggccat ggtgatgatc
      661 ggctccacct tgattcccgg cgtgtccatg ggtatcagca ggaaggtgat gcctgcctgg
      721 ggacgcacgc ccgtgtctgt gcgcaccagg cagaagatgt ggtcggcaaa ctgggcgtgg
      781 gtcgtccaga tcttggtgcc gttgacgatg tagtcgtcgc cgtccttgac tgcggaagtt
      841 ttcaggctgg aaagatcgga tccggacccg ggctccgagt agccctgaca ccagtagtgc
      901 tcgccggaca gcatgcgagg caggtagtag ttcttctgct cctcggtccc gtacttgaag
      961 atcacgggcg ccagcatgcg aagcccgaga gggatgaggc cgggggcgga cgcccgcgcg
     1021 cactcttcgc cccagatgta cttctgggtg acgctccatc cggtgccgcc gtgctcggcg
     1081 ggccacgccg gcacggccca ccctttctcc actagaatcg cctgccagcg catggcgagt
     1141 tccttgtcag cgaacaccgt cgtcgtcttg cgcaccgcct ggcgcatgtc cggcgtgacc
     1201 ttctcttcca ggaaggccct gacctcttcc cggaacgcct gatcttcctt gctgaactcg
     1261 aaatccacgg ctacttctcc ttactgtcta cgactgccct agacgccgac cctttccggc
     1321 cttcggctcc atgatgatct ttccgcgccc gtgacgggcg cgtgaagata aggacgcaac
     1381 gcgccgccgc ccggagcggg ccggcagcgc cggtctcaac gcacgacgat ggattcttcg
     1441 ttccagggac tcaaggcacg gctgtcgtcc tgaaacgctg ccggcagctc ttccaccatg
     1501 tgccaggtgg ccgccagcct gccgaacccc acgaagaacg ccacgtgcat ccccagctcc
     1561 acgatctcgc tttccgagaa gtgcttgcgc agctcgtcgt agacggcatc gtcgatggcc
     1621 agatgatcgg tcgccatgag ctccccgaac cggatggccg ctttctcggc atctgtgagg
     1681 ttcccggctt cggccggttt ctccagcgag cagaccagat cctcggtcac gccgtcgttg
     1741 agagcgtccg tgtaacggat cgccatgcag ctgcggcact ggttgaagaa cgcgacccgc
     1801 agccgcacga gctcgatgag cttgtcaggg agcgtccgct tggtcttgag cgcgccgcca
     1861 aactgcatca ggcccagcgt aagctcgggc cgatgagcga agaaccgggt caggccctgc
     1921 tccagagggg tcaggttctc aggaatgatc gcggccttga gctcaggcgc ccagtcttca
     1981 ggctcgattt tctgtagtcg actcatcgca tttccccttt gtctgttcgg gccgagaaaa
     2041 ggccctccgc ctgcacgtcg gcaagcgatg tctgaaacac atcgataaga gtatcaattt
     2101 ccgcgtcgga atgagcagcg gaaaggaacg cgcgctgggt cccgggcacc agaacgcccc
     2161 ggttcagcgc gtgcagatag aacgccttct ccgcggcgac gctcgcgccg gtgacgtcgc
     2221 gcatgctgcg gatgggctcc cgctgaaaga acatgtgaaa catgctcccc acggacttca
     2281 tctgggccgg gatctgccgt tcctggcaga agccgttcac cgcccccgcc agccgctcgc
     2341 cagcctgctc gagaccacgg tacacgtcgg ggtggtcgcg aaggtagccc accgcggccg
     2401 ctcccgcctc catggtgagc gggttgcccg agaacgtacc gccggagaac accccgttgc
     2461 tcgccccgag cccggtgaac acctccatca cgtccgcacg gccggtgatt gcccccacgg
     2521 gcaagccgcc gccgagtatc ttcccgtagg tggcgaggtc cggcctcacg ccgaatcttt
     2581 cctgagcacc gccgtaggcg acgcggaaac ccgtgatgac ctcgtcaacg gcaaacagaa
     2641 cgcccgcttc acggcagacg gcttcgagct cgcatagaaa ctcgccggcc ccgggctggg
     2701 gattcgagct ctgaacaccc tctaccatga ccagcgcaag ctcgtccgcg tgacggcgga
     2761 tcagatcgaa ggcggcctcg tgacgatacg gcagcagcag cgtcaggtct gccagcgccc
     2821 gcgggatgcc gcgccccatt ggcagcttgc gcggcgcttc ccgggggctt gtcgggtcgg
     2881 tcagccacat cccgtagtcg tgggcaccgt gatagaagcc gtcgaacaga gcgatcttct
     2941 ccttgccaga gtacccacgg gcagcacgga tcgcgtacat ggtcgcctcg gaacccgtgt
     3001 tgcagaaaac cacccgctcg gcgcaggggc tggcctcgtg caggagctcg gccagcttca
     3061 tctggctggt ggagtgaatg ccgaacatcc accctttctc cgtgcggtcc ttgatggcct
     3121 gctcgatcag cgggtgccga tggccgagga tgtgcaggcc gaagcccacg gaggtgtcga
     3181 tgtagcggtt gccgtccgca tcgaagacga acggcccctc gccgctctcg atgaagatcg
     3241 gatggggcat ctccaggttg gcagcgagcc ccgagttgag aatctcggcg cgggcctcgg
     3301 agatctcctg ggatccggcc gttctggcgg ccagctcggc gcgcgcttca gcgaccgtgg
     3361 tcatttgccg gaaaggcgcg cgaccaccgg cgcaaaggcc tccagattgt cctctccgac
     3421 ggtgaagtag gagatgccga actcctcgcg gcgacgctgc agctcgtcgc agatggcatc
     3481 aacgctgccg aagagaccgt gggggtggcg gagcatttcg tcctcggaca tgccgaacat
     3541 ctgcgccatg cccgcgacca cgggcttggc gtcgtcggtc accacggtga agtaggcgcc
     3601 gatctcgatc tccagatcgt cgaagcgatc gccggcgcct tcgcggatcc agccgatctt
     3661 cttgcgggtc tcctgctcgc tggacatctg cacgccgtcg ggcccgatca tgcccatgcg
     3721 gttgttgaag ttcaggctga cgatgtcggc ctcccgtccg gccaggcgca gcacccgggg
     3781 gctgccgccg ccgaccatga tgggagcgcg tcccacgggc ttgggcagac cttcgaagtc
     3841 cttccaggac agcgtgtcgt tgtggacatc gaccaggtcc tcgccgcgga acgccttcac
     3901 cccggcaatg acgtcggcaa gccggtcgat gcgcacgcgg ggctcgtcga agtcgatacc
     3961 caccgcttcg tactcgctct tcacccagcc ggcgcccaga cccagctcga gccggccgtt
     4021 ggacagcatg tcgatggtca tggcggactt gatcagcacc acgggcaggt gatagtcgat
     4081 gcagaacacc cggcaaccga tgttgatggt gctcgttacc gccgctgcaa acgccatggc
     4141 ggggatggcg gcgacgttct gaatcgggtg gttggctgcg gtcagcgccg gccccggccc
     4201 cagcagatga tcggccagat ggaacgacga gtagccgaga tcctcggcct tgcgagcctt
     4261 gtctgcccag ctctggcccg actccgcgtt gaacgtctgg aacccgaaac gaaacggctt
     4321 tgccatgctc ggctctccct ggcagctgcc cgctagacca cgcggtcgat gcagtacttg
     4381 at
//
LOCUS       DBNHOX010000779         3454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1605546, whole genome shotgun sequence.
ACCESSION   DBNHOX010000779 DBNHOX010000000
VERSION     DBNHOX010000779.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1605546"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="AC16UZ_16920"
     CDS             complement(<1..1105)
                     /locus_tag="AC16UZ_16920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR6248424.1"
                     /translation="MGGDAIDLGTMLLGLLGGLAIFLFGLDQLTNMLKVTAGARMGDL
                     LAKMTTNRFKGVLAGGVTTAVIQSSSVTTVLVVGFVSAGLMTLQQSIGVIMGAEIGTT
                     ITAQIIAFKITEYALVAVVVGFVMQAFFDNDLVKRYGTMVFGLGLVFFGMFMMGEATR
                     PLRDYPPFVDLLAQMDNRVLTILLSAGFTALIQSSSATTGVIIVLGSQGLISLEQGIA
                     LVFGANIGTCVTAVLASIGKKHRGLQVALVHVTFNVAGVLMWFAFIDELAAFSRWLSP
                     ASPGLVGADRLAEEIPRQIANAHTLFNVTNTLIFIWFTGAFAWAVTRLAPGKRSSGAG
                     IFEPRYLDSALVQTPALALDAARREIVRMGDHLV"
     gene            complement(1180..3267)
                     /locus_tag="AC16UZ_16925"
     CDS             complement(1180..3267)
                     /locus_tag="AC16UZ_16925"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6248425.1"
                     /translation="MLLACLLLALGFSVEAEIASPDAARRGEALYGEHCASCHLLSLR
                     GSAHGAALRGPGFRDRWQGTSTSELLRYNQANMPPGESASLRDEEHVAIVTYLLEAND
                     WLDATSPFDFSTPRRIGGGADPEPDSGEALDSWGEASSIDAIARSRSAFVNRKVQKYV
                     PVTDAMLASPPADDWLSWRRTLDGQGYSPLDDINRETVGKLKLAWVLAMRDGSNQVTP
                     LVHDGVMYLTHARNLIQAVDGSSGDVIWEYAYEYPEAARTLGGPLRNIAIYGDKLFMA
                     TYDAALVAVDARTGEQVWRAVKADYRQGYTHTSGPIVGDGVVLSGINGCERYKEDGCF
                     VTGHDADTGRELWRTSTIALPGDVNDASWGGLEPSFRAGGDTWIAGSYDPKLKLFFIG
                     TSRAKPWVAASRGMTVDEAALYTNSTLAMDPRTGKIVWYFQHVPGETIDMEVGFERVL
                     VDVEGARLLFTVGKDGILWKLDRATGQYVAHVETIEQNIFEHVDPETGRVRYREDIRS
                     AGVGETFSACPGIYGGHNWQAMAYSPQARSLVIPLHRLCADMVGRPVESKVGEGGFGG
                     DSRSYEMPGSNGNLGRLVAVDAATLEERWVHEQRAMFMTGALTTAGGLVFIGDLDRYV
                     KAFDVADGRLLWQARLGAPTHGYPVTYRAGGKQYLAVPTGMGVFRALTAVISPDIYQP
                     ANGQALYVFELPN"
BASE COUNT          666 a         1160 c         1066 g          562 t
ORIGIN      
        1 gcacgagatg gtcgcccatc ctgacgatct cccgccgagc ggcgtccagg gcaagggccg
       61 gcgtctgaac cagggccgag tccaggtaac gcggctcgaa gatcccggcg cccgagctgc
      121 gcttccccgg cgcgagccga gttaccgccc acgcaaatgc cccggtaaac cagatgaaga
      181 tcagcgtatt cgtgacgttg aacagcgtat gggcgttggc gatctgcctc gggatctcct
      241 ccgcgagacg atccgcaccg accaaccccg gagacgcagg agacagccat cgcgagaagg
      301 cggcaagctc gtctatgaag gcgaaccaca tcagcacccc ggcaacgttg aaggtgacgt
      361 ggaccagggc gacctggagc ccgcggtgtt tctttccgat ggaagccagc acggcggtca
      421 cgcaggtgcc gatgttggcg ccgaaaacca gagctatccc ctgctcgagg gaaatgagcc
      481 cctggctgcc cagcacgatg atcacgcccg tggtcgccga cgagctctga atcagggccg
      541 taaatccggc gctgaggaga atcgtgagaa cccggttgtc catctgcgcc agcaggtcga
      601 cgaaaggggg gtaatcccgc agcggccgcg tcgcttcgcc catcatgaac atgccgaaga
      661 agacgaggcc caggccgaag accatcgtcc cgtagcgctt gaccagatcg ttgtcgaaga
      721 acgcctgcat gacgaacccc accaccacgg cgaccagggc atactcggtg atcttgaagg
      781 caatgatctg agcggtgatc gtggtgccga tctccgcgcc catgatgacg ccaatggact
      841 gctgcagcgt catcaggccc gccgacacga acccgacgac gagcaccgtg gtgaccgacg
      901 acgattggat cacggccgtg gtcacaccgc cggccagaac ccccttgaac cggttcgtcg
      961 tcatcttggc cagcaggtca cccatgcgcg caccggcggt gaccttgagc atgttggtga
     1021 gctgatcgag cccaaacaga aaaatcgcca gcccgccgag aaggccgagg agcatggttc
     1081 ccaggtcgat ggcgtcaccc cccatgctcg ctatctcacc cgactcatcg tgaccgcccc
     1141 gtcacgttgg ccgcgagtgc ccaggcgctg aaggagcgct cagttcggca attcgaatac
     1201 atacaacgcc tggccgttgg caggctggta gatgtcgggg ctgatgaccg cggtgagcgc
     1261 cctgaaaacg cccatcccgg taggcaccgc cagatactgc ttcccgcccg cacgataggt
     1321 gacgggatac ccgtgtgtgg gcgcgccgag ccgtgcctgc cacagaaggc gcccgtcggc
     1381 gacgtcgaat gccttgacgt agcggtccag gtcaccgatg aacacgagac cacctgccgt
     1441 ggtcagggcc ccggtcatga acatggcccg ctgctcgtga acccaacgct cctccagggt
     1501 ggcagcatcc accgcaacca ggcggcccag gttgccattg ctgccaggca tctcgtagga
     1561 gcgtgaatca cccccgaacc cgccttctcc caccttcgac tccacggggc gcccgaccat
     1621 gtcggcacag agccgatgca gcggaatgac gaggcttcgc gcctgggggc tgtaagccat
     1681 cgcctgccag ttgtgaccgc cgtagatacc cggacaggcg ctgaacgtct ccccgacgcc
     1741 agcggaccgg atgtcctcgc gatagcgcac ccgaccggtc tcgggatcga cgtgctcgaa
     1801 gatgttctgc tcgatggttt ccacatgagc cacgtactgc cccgtcgccc gatcgagttt
     1861 ccagagaatg ccgtccttgc cgacggtaaa cagcagccgt gcgccttcga cgtccaccag
     1921 cacccgctcg aaccccacct ccatgtcgat ggtttcaccc ggaacgtgct ggaagtacca
     1981 gacgatcttc ccggttcgcg gatccatggc cagcgtggag ttggtgtaca acgccgcttc
     2041 gtccaccgtc atgccccgac tcgcagctac ccagggcttg gcccgggacg ttccgatgaa
     2101 aaagagtttc agcttcggat cgtagctgcc ggcaatccag gtgtcgccgc ctgctctgaa
     2161 cgacggctcg agtccgcccc agctggcgtc gttgacgtcc ccgggcaatg cgatggtgga
     2221 cgtccgccac agctcacgac ccgtatccgc atcatggccg gtaacaaaac atccatcctc
     2281 cttgtaacgc tcgcacccgt tgatgccgct gagaaccacg ccatcgccga cgatgggccc
     2341 gctggtatgt gtatagcctt gtctgtaatc ggctttgacc gcgcgccaga cctgctctcc
     2401 ggtacgggca tccacagcca cgagagcggc gtcgtaggtc gccatgaaga gcttgtcacc
     2461 atagatggca atgttacgca ggggaccgcc aagagtacgc gcggcctcgg gatactcgta
     2521 ggcgtactcc cagatcacat ctcccgacga gccatcgacg gcctggatca ggttgcgggc
     2581 atgggtgagg tacatcaccc cgtcgtggac gaggggcgtg acctggttgc tgccgtcgcg
     2641 catggcgagc acccaggcga gtttcagctt gccgacggtc tcccgattga tgtcgtccag
     2701 cgggctgtag ccctgcccgt ccagcgttcg gcgccagctg agccagtcgt ctgccggggg
     2761 gctcgcgagc atcgcgtcgg tgacgggaac gtacttctgc accttccggt tgacgaaagc
     2821 agaccggctg cgggcgatgg cgtcgatgct ggaggcctcg ccccacgagt cgagcgcctc
     2881 gcccgaatcc ggctcggggt ccgcgccgcc gcctatccgg cgcggcgtcg agaagtcgaa
     2941 gggcgacgtc gcgtccagcc aatcgttcgc ttccagcaga tacgtaacga tggccacgtg
     3001 ctcttcgtca cgcagcgatg cggattcgcc cggcggcatg ttggcctggt tgtagcgcag
     3061 cagctccgag gtggacgtgc cctgccagcg atcccggaaa cccggccccc gcagcgcggc
     3121 gccgtgagcc gaaccccgca gggacagcag atggcagctc gcgcagtgct cgccgtagag
     3181 cgcctcgccg cgtcgcgcgg catcggggga agcgatttca gcttcaaccg aaaatccaag
     3241 cgccaacaac aggcaggcga gcagcacatg acctggtcga cgataagcgg gcaccagccg
     3301 cccgcattct tgccgactac cgttgggccg caagatcatc gagtgccaga acgaggggaa
     3361 atttgtcttc cggatacccg tcgatcacgc ggggttgacc gaaccactcc accgccgggc
     3421 ccacctcggc aagagacagg aggggcttca acag
//
LOCUS       DBNHOX010000780         1689 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_448907, whole genome shotgun sequence.
ACCESSION   DBNHOX010000780 DBNHOX010000000
VERSION     DBNHOX010000780.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_448907"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="AC16UZ_16930"
     CDS             <1..531
                     /locus_tag="AC16UZ_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248426.1"
                     /translation="VGAGATFIARAVDVDQKVLPGILNAAREHKGAALVEILQNCIVY
                     NKDVFEDVVAKKTAADTQVVVEHGHPLVFGKERDKGLRLNPQTLALEAVTLGENGVSE
                     ADLVVHDEINTALAHMLLSLAPPNPVAMGIIHRRRAPAFDAAYWSSKPTGRSARVAEL
                     LRHGNVLDHRRSPQGR"
     gene            695..1423
                     /locus_tag="AC16UZ_16935"
     CDS             695..1423
                     /locus_tag="AC16UZ_16935"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR6248427.1"
                     /translation="MAKHEEIAESLTRDILSGQYRTGERLPSERDLSVRFEANRGAVR
                     EAMKKLEQLGIANIQPGGARVAPLEEASLDVIGHMLALGDLPDSKLVIHIIEVISALV
                     RLAVESALNTATDEDIERLREHNRPLFEEDLDDLAHMEARLELMGAIMSASDNLVCQL
                     IARSLLAQLTPRTEPLKAFAQFDTEAHADNARRLDEALAKRDADALRHVFTESTALTR
                     ESVLRSFEAYDAANRAPLTGVAAS"
     gene            1423..>1689
                     /locus_tag="AC16UZ_16940"
     CDS             1423..>1689
                     /locus_tag="AC16UZ_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248428.1"
                     /translation="MKKLLVPGLVILASLFGAVTLMATAPELEPNAIEPLATTVRVVE
                     VIPQPVRLTVHSQGTVVPNMESELIPEVSGRVEWISPSLVNGGYF"
BASE COUNT          341 a          568 c          521 g          259 t
ORIGIN      
        1 gtcggtgcgg gagccacctt catcgccaga gccgtggacg tcgatcagaa ggtattgccg
       61 ggaatcctca acgcggcgcg ggaacacaaa ggcgccgcgc tggtggagat cctccagaac
      121 tgcatcgtct acaacaaaga cgtcttcgag gacgtggtcg ccaagaaaac ggcggcggac
      181 actcaggtcg tcgtggagca cggccatccg ctcgtgttcg gcaaggagcg cgacaagggg
      241 ttgcgcctca atccgcagac cctggcgctg gaagcggtga cgctcggcga gaacggcgtg
      301 agcgaggccg atctggtggt ccacgacgag atcaataccg ccctggcgca catgctgctg
      361 tccctggcac caccgaaccc ggtggccatg ggcatcatcc accgacgtcg cgcgccggcc
      421 ttcgacgccg cctactggtc gagcaaaccg accgggcgca gcgcccgggt cgccgagttg
      481 ctgcgccacg gcaacgtcct ggatcaccgc cgctctcctc agggccgctg accgccgccc
      541 atccccaccg cggccactgg ccgcggtttc gcgaacgcgc gcacatttcg ttaccaaagg
      601 taacgaaatg ctggacatgc gaacctgatt gactaaaatg ccccgaccat catggtcaga
      661 ccaacatggt cgcaccagtc agaacaggaa gaacatggca aagcacgagg aaatcgccga
      721 gagcctcacc agggacattc tgagcggcca gtaccgcacc ggcgagcggc tgccctcgga
      781 acgggatctt tccgtgcgct tcgaggccaa ccgcggcgcc gtccgggagg ccatgaagaa
      841 actggagcag ctcggcatcg ccaacatcca gcccggcggc gcgcgggtgg cgcccctcga
      901 agaagccagc ctggacgtga tcggccacat gctggccctg ggcgaccttc ctgacagcaa
      961 gctggtgatt cacatcattg aagtcatttc cgcccttgtc aggcttgcgg tggagtcagc
     1021 gctgaatacg gccaccgacg aagacatcga gcgcctgcgc gaacacaacc gaccgctctt
     1081 cgaggaggat ctggacgacc tcgcccacat ggaggctcgc ctggagctga tgggcgcgat
     1141 catgagcgcc agcgataatc tcgtatgcca gctcatcgcc cgcagcctgc tggcacagct
     1201 cacgccgcga accgagccgc ttaaggcatt cgcccagttc gacacggagg cccatgcgga
     1261 caacgcccgg cgactagacg aagccctggc caaacgtgac gccgacgctc tgcgccacgt
     1321 gttcaccgag agcactgcgc tcacgcgcga atccgttcta cggtccttcg aagcctacga
     1381 cgcggccaac cgcgcaccgc tcacgggggt agccgcgtca tgatgaagaa gctgctcgtt
     1441 ccgggactcg tcatcctggc atccctcttc ggagcggtaa cgctgatggc aacggccccc
     1501 gagctggagc ccaacgccat agagcccctg gccaccacgg ttcgagtggt ggaggtgatt
     1561 ccccagccgg tccgcctgac cgtgcactcc cagggtaccg tggtaccgaa catggagagc
     1621 gagctgatcc cggaagtgtc cggacgcgtc gagtggatct cgccgtcact ggtcaatgga
     1681 ggttacttc
//
LOCUS       DBNHOX010000781         4319 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1284186, whole genome shotgun sequence.
ACCESSION   DBNHOX010000781 DBNHOX010000000
VERSION     DBNHOX010000781.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1284186"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="AC16UZ_16945"
     CDS             <1..340
                     /locus_tag="AC16UZ_16945"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR6248429.1"
                     /translation="AIQRVLRISGSGLATSGDYRNFLEVDGERFAHTIDPRTGYPVDH
                     TLASVTVVHESAMLADGYATLLNVLGPQEGFSLAEGLGLPVLFLIRTPNGFEERYTVG
                     LEQFLGQTDY"
     gene            342..566
                     /gene="nqrM"
                     /locus_tag="AC16UZ_16950"
     CDS             342..566
                     /gene="nqrM"
                     /locus_tag="AC16UZ_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HIR6248430.1"
                     /translation="MTILLTFLCMMAVVGLMAIGVMFGRRPIAGSCGGLKNLGIGGEC
                     EICGGDPARCESPGDTRLAVDATNRARRSR"
     gene            647..1870
                     /locus_tag="AC16UZ_16955"
     CDS             647..1870
                     /locus_tag="AC16UZ_16955"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6248431.1"
                     /translation="MIKAVALSVGSRYAFSRRSTLSFISLVAISGLALSVAVLVIVTS
                     VVNGFDRELRERVFGVLPHLSFVGREPLAFSLDDLEALRAIPGVRGIAPFVEGAALVA
                     VADGVGAVMVTGMEPESYESVSDVHKFVRGRMPQAGEYGVLLGDQVAQRLGVAIGDAV
                     TLVLPSATVTPAGLFPRQKRFVITGLLDSQSDLDGRAAYVRLTVAQKLFRLGDRIHGY
                     QARLDDLFAALEVGSAGVLALGPQRLFARSWMRTHGNLYQAIGMLKSTLFVLLSFLIA
                     VAAFNLISTLVMVVDQRSGDIAVLRTLGSDTSTLIWTFVILGVLLGGAGIAAGLGLGA
                     ALALALPGLYEWLTTLLGADLLAQYFVTYLPVELQPGDLLGIAVTAFLLCVLSTLYPA
                     RRAAGLQPFKVLAYE"
     gene            1863..2537
                     /locus_tag="AC16UZ_16960"
     CDS             1863..2537
                     /locus_tag="AC16UZ_16960"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6248432.1"
                     /translation="MSEPVLEARGLVKSFTQGTTRVVVLSGADLDVVAGERVAIVGRS
                     GSGKSTLLHLLAGLDDADAGSVIVGGKDMTAGSPAQRAHIRNAHMGFVYQFHHLLPEF
                     SALENVIMPLRLGGMDPAAAAERARGMLERVGLAERFEHRPSALSGGERQRVAVARAL
                     VGSPLVVLADEPTGNLDRDNAEHVFRMMCELSDEHGVGFVIVTHDEEMLGSMHRVLRL
                     ENGRLA"
     gene            2534..3742
                     /locus_tag="AC16UZ_16965"
     CDS             2534..3742
                     /locus_tag="AC16UZ_16965"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR6248433.1"
                     /translation="MSTSLWIALRYLVTRRRQFAAFVTWVSVAGLALGVLVLTVVVSV
                     MNGFDAELRHRILGSVPHLIIEGESVTDPNVGSLLDRPEIVGSFDFFLGSGMVTRSGA
                     VHPVTVYGLDAEGLTGLGEIGQAMRSGRLSGLFEIPRGIVIGEPLAAHLGLLPGDSVA
                     VVISEPTASGVQPRFGRYELVGTFEVGAVIDYSVVVVARDALDGLRLDEVGVNGARLV
                     LADPLDAPRLAAELAMARPAWNLVSWTDSYGELFQAVGLEKMVMFIILLMVVAVAAFS
                     IVSGQMMAVADKRADIAILSTMGASRRTILGIFLLQGVLVSGLGIAVGLALGTLTAEH
                     IGAVIAGVEDLLGFRFLAGTYFVQVPSEVEVADLVIIGVMSSCLCLASAWLPAHRAAI
                     MNPLEGLHPG"
     gene            complement(3708..4217)
                     /locus_tag="AC16UZ_16970"
     CDS             complement(3708..4217)
                     /locus_tag="AC16UZ_16970"
                     /inference="COORDINATES: protein motif:HMM:NF021363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HIR6248434.1"
                     /translation="MRKWLKEPEIWHLHRRSVAGAAFIGMFCAFLPVPFQMLIAAVLA
                     VASRCNLPMSVVLVWITNPLTIPPMFYFAYRLGAWLLNMEIEVATIELSWEWLKTHIG
                     RIGYPLIVGSLVCGWVSGLTAMVVVRIAWRMHVIKRWRERRRRRLERQMATDDSGGVT
                     PDEAPRAGS"
BASE COUNT          628 a         1302 c         1482 g          907 t
ORIGIN      
        1 cgcgattcag cgggtgcttc ggatcagcgg gtctggcctt gccacttccg gcgactatcg
       61 caattttcta gaggtcgacg gcgagcgctt tgctcacacc atcgatcccc gcacgggcta
      121 tccggtcgac cacaccctcg cctcggtgac cgtggttcat gaatccgcca tgctggcgga
      181 cggttacgcc acgcttctca acgtcctggg gccgcaagag ggcttttccc tggccgaggg
      241 gctggggtta ccggtacttt tcctgatccg aactccgaac ggcttcgaag agaggtatac
      301 tgtcggcttg gagcagtttc tcgggcaaac ggattattga cgtgacgatt cttctgacct
      361 ttctctgcat gatggcggtg gtggggctca tggccatcgg cgtcatgttc gggcgccggc
      421 ccatcgcggg cagctgtggt gggctgaaga acctggggat cggcggcgag tgcgagatct
      481 gcggcggcga cccggcgcgc tgcgaatcgc ctggggacac ccgtcttgcc gtggacgcaa
      541 ccaaccgggc acgacgctcc cgctgattac cagggcttct ccccgagggc gcgcggcgct
      601 accgacgtgt tagcatgcgg cctcccccgg gccgggtcgg tttgcattga tcaaagcagt
      661 tgcgctcagc gtgggcagtc gctacgcctt ctcgagacgg agcacgcttt ccttcatcag
      721 cctggtcgcc atcagcgggc tcgccctcag cgttgccgtg ctggtgatcg tgacgtcggt
      781 cgtgaacggc ttcgacagag aactccgcga gcgggttttc ggcgtgcttc ctcatctgag
      841 cttcgtcggg cgcgagccgt tggcattcag cctcgatgat ctggaggcgc ttcgggccat
      901 tcccggcgtc cgggggatcg ccccattcgt tgaaggcgcc gcgctggtcg cggtggccga
      961 cggggtgggc gccgtgatgg tcacgggcat ggagccggaa agctacgaga gcgtctccga
     1021 cgtgcataag tttgttcgcg gccgcatgcc tcaagccggc gagtacgggg tattgctcgg
     1081 tgatcaggtg gcccagcggc tcggcgtggc cattggcgat gctgtcaccc tggtgctgcc
     1141 gtcggcgacg gtcacgcctg cggggctgtt cccgcggcag aaacgctttg tgattacggg
     1201 gctgctcgac tcgcagtccg acctggacgg tcgcgcggcc tatgtgcgcc tgaccgtggc
     1261 gcagaagctg ttccggcttg gcgatcgcat acacgggtat caagccaggc tggacgatct
     1321 gttcgctgcg ttggaggtgg gttcggcagg tgttctcgcc ctcggtccgc agcgcctgtt
     1381 cgcgcgttcc tggatgcgta cccatggcaa cctctatcag gccatcggca tgttgaagag
     1441 cacgctgttc gtcctgcttt cgtttctgat cgcggtggcc gcgttcaacc tgatctcgac
     1501 cctggtgatg gtggtggacc agcgaagcgg cgacattgcc gtgctgcgga cgctgggcag
     1561 tgacacctcg acgctgatct ggacgttcgt cattctcggc gttctgctcg gcggtgccgg
     1621 catcgccgcg ggtctcggcc tgggcgccgc gctggctctg gctcttcccg gcctgtatga
     1681 gtggctgacg acgcttctgg gagccgatct gctggcccag tacttcgtta cctacctgcc
     1741 ggtcgagctg cagccgggcg atcttctcgg catcgccgtg acggcctttt tgctgtgcgt
     1801 tttgagcacc ctgtacccgg cgcggcgcgc ggcggggtta cagcctttca aggttcttgc
     1861 gtatgagtga gccggtcctc gaagcccgcg gcctcgtcaa gtcgttcact cagggcacga
     1921 cccgggtcgt ggtgttgtcg ggggcggatc tcgacgttgt cgccggcgag cgggtcgcca
     1981 tcgtcggccg gtccggctcg ggcaagagca ccctgttgca tctgctggca ggtctcgacg
     2041 atgccgacgc ggggtcggtg atcgtcggcg gcaaggacat gacggcggga tctcccgcgc
     2101 agcgggcgca catccgcaac gcgcacatgg gcttcgtcta ccagtttcac catctgctgc
     2161 cggagttctc ggctctggaa aacgtcatca tgcctctgcg gctcggaggc atggaccctg
     2221 ccgccgccgc agagagagcg cgcggtatgc tggagcgagt gggtctggcc gagcggttcg
     2281 agcatcggcc atcagctctg tcgggcggag agcgccagcg ggtggccgtt gcccgcgcgc
     2341 tggtgggcag tccgctggtg gtgctcgccg acgagccgac ggggaacctc gaccgggaca
     2401 acgcggagca cgttttccgc atgatgtgcg agctcagcga tgagcatggt gtgggttttg
     2461 tcatcgtcac ccacgacgaa gagatgctgg gcagcatgca ccgagtcctg cgtctggaaa
     2521 acggccggct ggcgtgagca cgtcgctctg gattgctctg cgctatctgg tcacgcggcg
     2581 ccgccagttt gccgctttcg tcacgtgggt ctccgttgcc ggtctggcgc tgggcgttct
     2641 ggtgctgacg gtggtcgtca gcgtgatgaa cgggttcgat gccgagttga ggcaccgcat
     2701 tctcggatcc gtgccccatc tcatcatcga aggggagagc gtcaccgacc cgaatgtcgg
     2761 ttcgctgctc gaccggccgg aaatcgtcgg ctctttcgac ttcttcttgg gttcgggaat
     2821 ggtcacccga agcggcgcgg ttcatccggt gaccgtctac ggcctggacg cagaaggact
     2881 caccgggctt ggagagatcg gccaagccat gcgcagcgga cgtttgtccg gcctgttcga
     2941 gatcccgcgg ggaatcgtga ttggtgagcc gctggcggcg cacctgggcc tgctgccagg
     3001 cgattcggtg gctgtggtga tctccgagcc gacggcgtcc ggcgtgcagc cacgctttgg
     3061 tcgctacgag ctggtcggta ctttcgaggt cggcgccgtc atcgactact cggtggtcgt
     3121 tgttgcccgc gacgcgcttg acggcttgcg tttggacgag gtgggcgtca acggggcgcg
     3181 cctggttctg gcggatcccc tcgatgcgcc gcggctcgca gcggagctgg cgatggctcg
     3241 gcctgcatgg aatctggtca gctggacgga cagctacggc gagctctttc aggcggtggg
     3301 cctggagaag atggtgatgt tcatcattct gctcatggtg gttgcggtgg ccgcgttcag
     3361 catcgtatcg gggcagatga tggcggtggc cgacaagcgc gcggacattg cgattctgag
     3421 cacgatgggc gcttcgcgaa ggacgatcct cggcatcttc ctgctgcagg gagttttggt
     3481 ctctggcctt ggcatcgccg tcggcctggc gctcgggact ttgacggccg agcatatcgg
     3541 tgccgtgatc gcaggcgtcg aggatcttct cggttttcgc tttctcgccg gcacctattt
     3601 tgtccaggtg ccctcagagg tcgaggtagc cgatctggtg atcatcggcg tcatgtcgtc
     3661 gtgcctttgc ctggcttcgg cctggttgcc cgcgcaccgg gccgcgatca tgaacccgct
     3721 cgaggggctt catccggggt gacgccgccg gagtcgtcag ttgccatctg gcgctccaga
     3781 cgccgccgcc gtctttcgcg ccaccgtttg atgacgtgca tccgccaggc gatgcgaacg
     3841 actaccatgg cggtgaggcc gctgacccac ccgcagacca gggatcctac aatcagcgga
     3901 tagccgatgc gtccgatgtg ggttttgagc cattcccagc tgagctcgat cgtggccacc
     3961 tcgatctcca tgttcagcag ccatgcgccc agccgatagg cgaagtagaa catgggtggg
     4021 atggtcagcg ggttcgttat ccagaccagg acgacgctca tgggcaggtt gcaacgtgac
     4081 gccacggcaa gcacggccgc gatgagcatc tgaaacggca ctggcaggaa agcgcagaac
     4141 atgccgatga aagccgcgcc ggcaaccgaa cggcggtgca ggtgccagat ttccggttcc
     4201 ttgagccatt ttcgcacggg gcgcagcgcc cgatgctcac tgagaaccgt cgggtttggc
     4261 aggtgcttgc ggagaaactt ttttggcata acggaaatta tgcacccgat ggaaacctt
//
LOCUS       DBNHOX010000782         2476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1092101, whole genome shotgun sequence.
ACCESSION   DBNHOX010000782 DBNHOX010000000
VERSION     DBNHOX010000782.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1092101"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="AC16UZ_16975"
     CDS             complement(<1..637)
                     /locus_tag="AC16UZ_16975"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR6248435.1"
                     /translation="MILSTDGGGSRGLIAANVLYHMEKKAEIPIRETFDFSAGVSTGA
                     MVAAYLARNAGTMEEVAKKAYSSENLSKIMNKSIWDRILGRLQNQPKYDGQNERAYVS
                     SVTQDSRINDITDKFLLILAYDFINRELVTFKNNRGHDGWYNPSLTEVCDAASAAPTL
                     REIWVVSLGTGRPMHELSQDERDRIGEASRDWGIVGWVANGLLDHMMSASSC"
     gene            complement(687..1010)
                     /locus_tag="AC16UZ_16980"
     CDS             complement(687..1010)
                     /locus_tag="AC16UZ_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248436.1"
                     /translation="MIEGHLNAFHLGSLDRIGIQASQHIAPFDQVALVDQKLGHDGNL
                     GRPDELKDAVVGLYPAQARDPAGGCIQRHSHQNHGQKERTGSKRRHASPCGIFPAFVV
                     RYVSG"
     gene            939..1151
                     /locus_tag="AC16UZ_16985"
                     /pseudo
     CDS             939..1151
                     /locus_tag="AC16UZ_16985"
                     /inference="COORDINATES: protein motif:HMM:NF052759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1062..1313)
                     /locus_tag="AC16UZ_16990"
     CDS             complement(1062..1313)
                     /locus_tag="AC16UZ_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248437.1"
                     /translation="MLSEADLRASQPADDGEQRALAGTGRSGQHHQLADALAFTRMDG
                     QDLAVYRSFDPRPGELGARGDQFGLVDLDVPLRFVERRF"
     gene            1396..1677
                     /locus_tag="AC16UZ_16995"
     CDS             1396..1677
                     /locus_tag="AC16UZ_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248438.1"
                     /translation="MLADEPTASLDRESSEQVMSIVELLRQRQIFSGAELDLVVDMAI
                     AQAQAGNQTFQNEQDREALNAAVKAVAMADNVVTEEELAVIDELTKRQP"
     gene            complement(1711..2391)
                     /locus_tag="AC16UZ_17000"
     CDS             complement(1711..2391)
                     /locus_tag="AC16UZ_17000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6248439.1"
                     /translation="MVPEYPAALIFDCDGVLVDSEIVAQRIERAHLASIGLHYEQVEF
                     VHRFTGTSEPEFLRRVEDDALERLRRKLPDGIIDEMKRAVQAELERVLQPISGVRAML
                     ACWDGPKAVASSSTLAALRFKLGRTGLDTAFGAHVYSAELVGRGKPDPAVFLHAAARL
                     GLDPGRCAVIEDSVNGILGAKRAGMTAIGFIGGAHCVPGHADALREVGAQWTFSNFAE
                     LSEFLDLI"
BASE COUNT          468 a          792 c          777 g          439 t
ORIGIN      
        1 cgcacgagct ggcgctcatc atgtgatcga gtagaccgtt ggccacccaa ccgacgattc
       61 cccaatcccg cgacgcctcg ccgatgcggt cgcgttcgtc ctggctgagc tcgtgcatcg
      121 gcctgcccgt gcccagcgac accacccaaa tctctctgag ggtgggcgcc gcggacgccg
      181 catcgcaaac ctccgtcagg gacgggttgt accacccgtc gtggccccgg ttgttcttga
      241 aagtgaccag ctcccgattg atgaagtcgt aggcaaggat cagcagaaac ttgtcggtga
      301 tgtcgttgat ccgggaatcc tgggtcaccg acgagacgta tgccctctcg ttctgcccgt
      361 cgtacttcgg ctggttctga agcctgccaa gaatccggtc ccagatggac ttgttcataa
      421 tcttggacag gttctcggag gagtaggctt tcttggccac ctcttccatg gtgcccgcgt
      481 tgcgtgcgag gtaggcagcc accatggcac cagtgctcac gccagcggag aagtcgaacg
      541 tttcacgtat cgggatctcg gccttctttt ccatgtggta gagcacgttc gccgcgatca
      601 gaccccggga accgccaccg tcggtggaga gaatcaagat tgctcctttg ctttgcatac
      661 cgcccctctt gatcccattg tagccattag ccgctaacgt accgtacaac gaacgccggg
      721 aaaatgccgc agggggaagc atgacgacgt ttcgagccgg tgcgctcttt ctgaccatga
      781 ttttggtggc tgtgccgttg gatgcagccg cccgcggggt ctcgagcctg ggccggatag
      841 agccccacga cggcgtcttt cagctcgtcg ggccttccga gattgccgtc gtggccgagc
      901 ttctggtcga cgagggcgac ctggtcaaac ggggcgatgt gctggctcgc ctggatacct
      961 atgcgatcaa ggctgccgag gtgaaacgcg ttcaggtggc cctcgatcat gccaagcggg
     1021 tgctgaagcg ccagcaggag ctcaagaaga gctcgttcca gtcagaagcg gcgctcgacg
     1081 aagcgcagag ggacgtcgag atctaccagg ccgaactgat cgccgcgcgc gcccagctcg
     1141 cccgggcgag gatcaaagct ccggtagacg gccaggtcct ggccgtccat gcgcgtgaag
     1201 gcgagcgcat cggcaagctg gtggtgctga ccggaccttc cggttccggc aagagcacgc
     1261 tgctcaccat catcggcggg ctgcgacgcg cgaaggtcgg cctcggagag catacccaca
     1321 agcttcccgg acagctctcc ggcgggcaac agcagcgagc ggggatggcg cgcgctggta
     1381 aaccagcctc ggctcgtgct ggcggacgaa ccgacggcct ctctggaccg ggaatccagc
     1441 gagcaggtca tgtccatcgt cgagttgctg cgccagcgcc agattttttc cggggcggag
     1501 ctcgacctgg tggtggacat ggcaatcgcc caggctcagg cagggaacca gacattccag
     1561 aacgagcaag accgcgaagc gctgaacgcg gccgtaaagg ccgtcgccat ggccgacaac
     1621 gtggtaacgg aagaggaact tgccgtcatc gacgagctga ccaaacggca gccttgatcc
     1681 ggggcgcagc ccagccagag tcagcctctc tcagatcaaa tccaggaatt cactgagctc
     1741 ggcaaagttg ctgaatgtcc actgtgcgcc gacctcgcgc agcgcgtccg catgaccggg
     1801 cacgcagtgt gctccgccga tgaatccgat ggctgtcatt cccgcccgtt tcgcccccag
     1861 gatgccgttc acgctgtcct cgatcaccgc gcaacgccca gggtccaacc caagacgggc
     1921 cgccgcgtgc aggaacacgg cgggatcagg cttgccacgg ccgacgagct cggcagagta
     1981 gacgtgagca ccgaaggccg tgtcgagccc ggtacgaccc agcttgaagc gcagagccgc
     2041 taaggtgctc gatgaagcca ccgctttcgg gccgtcccaa caggctagca tcgcgcggac
     2101 gcccgagatc ggttggagca cgcgttcaag ctccgcctgc acggcgcgct tcatctcgtc
     2161 aatgatccca tccggaagct tcctgcgcag ccgctccaga gcgtcgtctt ccacccgccg
     2221 gaggaactcc ggctcggagg tgccggtaaa gcgatgaacg aactcgacct gctcgtagtg
     2281 aaggccgatc gaggccaggt gcgcacgctc gattcgctgg gcgacgatct cgctgtccac
     2341 cagcacccca tcgcagtcga agatgagcgc cgccgggtac tcagggacca aggggaacgc
     2401 tcctgtggat cacctggcac gaaccgtgta gaccatccgg cggcggcgtc attgatcgct
     2461 gcctgagtct gtttgg
//
LOCUS       DBNHOX010000783         1728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_385696, whole genome shotgun sequence.
ACCESSION   DBNHOX010000783 DBNHOX010000000
VERSION     DBNHOX010000783.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_385696"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1728)
                     /gene="parC"
                     /locus_tag="AC16UZ_17005"
     CDS             complement(<1..>1728)
                     /gene="parC"
                     /locus_tag="AC16UZ_17005"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HIR6248440.1"
                     /translation="KPVQRRIIFAMSELGLSNQAKFTKSARTVGDVLGKFHPHGDSAC
                     YEAMVLMAQPFSFRYPLVDGQGNWGAPDDPKSFAAMRYTEARLSAYAELMLSESKQGT
                     VDFIANFDGTLTEPRLLPARAPFVLLNGSTGIAVGMATDIPPHNMNEVISACIHLLDS
                     PKATVADLMTHIKAPDFPSEAIIITPPAEIREAYETGRGSVKARAVWKKEAGDIVVTA
                     LPHQASPAKVLEQIAQQMNAKKLPMLADLRDESDHENPTRLVLVPRSNRVDAERLMSH
                     LFATTDLEKSFRINLNIIGLDQRPQVKSLQAILKEWLTYRLETVRRRLTFRLDRINER
                     LHILDGLLKAYLNLDEVIRIIREDDHPKASLMKRFTLSEIQANAILDLRLRQLAKLEE
                     DKLKAEKSELESEKADIEKTLKSKARMKALLKSELVEIAEGYGDARRSPLGAVEDAAA
                     YSEEDLISNEPITVVLSEKGWVRSAKGHETNPEELSYRSGDGYGHAARGRTNDILVFV
                     DSTGRCYTVAAHSLPSARGQGEPLTGRLKPPAGSRFVGVALGPGESKVLLASDAGYGF
                     VGRLSGLVA"
BASE COUNT          281 a          545 c          562 g          340 t
ORIGIN      
        1 tggcgacgag ccccgacagc cggccgacga aaccgtagcc agcgtcgctt gccagcagca
       61 ccttcgactc gcccggtccg agggcgacgc ccacgaacct cgatcccgcg ggaggtttga
      121 gccgcccggt gagcggctcg ccctgtcccc gggcagaggg gaggctgtga gccgccacgg
      181 tatagcagcg gcccgtgctg tccacaaaga ccagaatgtc gttggttcgg ccccgggccg
      241 cgtgcccgta gccgtcgccg ctccggtagc tcagctcctc ggggtttgtt tcgtggcctt
      301 tggcggaccg gacccatccc ttctccgaga gcaccaccgt gatcggctcg ttgctgatca
      361 gatcttcctc gctgtaggcc gccgcatctt ccaccgcacc cagaggcgat cggcgagcgt
      421 cgccgtaacc ctcggcgatc tcgaccagtt cggacttcaa cagggccttc atgcgggctt
      481 tcgatttgag cgtcttctcg atgtccgctt tttccgactc gagttccgac ttctcggcct
      541 tgagcttgtc ctcctccagc ttggcaagtt gccgcaggcg aaggtccagg atggcgttgg
      601 cctggatttc cgacagcgtg aaccgcttca tcagggaagc cttcgggtga tcgtcttccc
      661 ggatgatccg gatcacctcg tcgaggttca gataggcctt gagcaggccg tcgaggatgt
      721 gcagtcgctc gttgatccgg tcgagacgaa acgtgagccg ccggcgcacg gtttccaggc
      781 gataggtcaa ccactccttg agaatggcct gcaaggattt cacctgaggg cgctggtcca
      841 gaccgatgat attcaggttg atccggaaac ttttctccag atccgtggtg gcaaacaggt
      901 ggctcatcag acgctcggca tccacccgat tggaacgagg caccaggacc agccgggtcg
      961 ggttctcgtg gtccgactcg tcacgcagat ccgccagcat cggcagcttc ttcgcgttca
     1021 tctgctgggc gatctgctct agcaccttag ccggcgacgc ctgatggggc agcgccgtga
     1081 cgacgatgtc cccggcctcc tttttccaca ccgcgcgcgc cttcaccgat ccgcgcccgg
     1141 tctcgtaggc ctcgcggatc tctgccggcg gggtgatgat gatggcttcg gacgggaagt
     1201 ccggagcctt gatgtgcgtc atgaggtcgg caacggtggc cttcggcgag tcgagcaggt
     1261 gaatgcaggc gctgatgacc tcgttcatgt tgtgcggcgg gatgtcggtg gccatgccca
     1321 ccgcgatgcc cgtgctgccg ttgagcagca cgaagggcgc ccgggccggc agaagccggg
     1381 gctcggtgag cgtgccgtcg aagttggcaa tgaagtccac cgtgccctgc ttggattccg
     1441 aaagcatgag ctcggcgtag gcagacaggc gcgcttccgt gtagcgcatg gccgcgaaag
     1501 acttggggtc gtcgggcgcg ccccagttgc cctggccgtc aacgagcggg tagcggaagg
     1561 aaaacggctg ggccatcagc accatggctt cgtaacacgc gctgtcgccg tggggatgga
     1621 atttgcccag cacgtcgccg accgtccggg ccgatttggt gaacttggcc tgattcgaaa
     1681 ggcccagctc gctcatggca aagatgatcc ggcgctggac gggcttga
//
LOCUS       DBNHOX010000784         7297 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_770730, whole genome shotgun sequence.
ACCESSION   DBNHOX010000784 DBNHOX010000000
VERSION     DBNHOX010000784.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7297)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_770730"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="AC16UZ_17010"
     CDS             complement(<1..1091)
                     /locus_tag="AC16UZ_17010"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR6248441.1"
                     /translation="MNTALTRSRLTGTLCLVLIAGGCASVPERNPVPPSLADAAEVPG
                     MPGIRYWADDAPPYAKAWLERSQEDLLSRYPASFGNPHNYLAISGGGANGAFGAGLLN
                     GWTDSGTRPEFTVVTGISTGALIAPFAFLGPAYDHVLKRVYTEFSTEDLLESRGLLQA
                     LTGEAATDSAPLRARIAEFVDEEVMDAIADEYQRGRSLLITTTNLDAGRSVTWLIGRI
                     AASGSPNALELIRDVMLASASIPAALPPVVVEVEAGGQTYDELHVDGNTSSVVNLYPL
                     ALDWEAVLDRLEVDEAPNLYIIRNGHIDITHQSVNRQTIAIAGRSIQTLMNYLARGDL
                     YELYLLTQRDGVRYHLANIPAEFTEAPTEP"
     gene            complement(1191..2570)
                     /locus_tag="AC16UZ_17015"
     CDS             complement(1191..2570)
                     /locus_tag="AC16UZ_17015"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase family protein"
                     /protein_id="HIR6248442.1"
                     /translation="MNILGMLLTLILLMTWSSPSRAEDEPARVLFTNVNVFDGYAKDL
                     QRGMHVLVEGNLIKTISDQPIAAADAQVIDGGGRTLLPGFIENHAHLMLMGPSLPAME
                     ANTTWEDFAIHGTRMAEMYLMQGFTTVRDAGGANAGLRRAIDSGQIVGPRFYPSAAFL
                     GTRGGHADFANYTSPVGESTNFGRLNLAQEVDDVADVQKFGRNNFRMGATQLKYMQSG
                     GVVSAFDPWQLLAGSQEEIKAAVQVANEYGSYVMAHSYRKEAIMNALNAGVKSIEHGF
                     AFDCEIAKLMKKKSAYITTNLTAFDPGLLEIPAVASVPSSLAKAKSATATFAGYIDNM
                     KKCPVKRGFQTDCVGSVAACNIQIAYEKHLNNEFFGPHTSLVAMTSVGGEIAALSGDF
                     MNPYPDAKLGIIEEGAYADILIIDGNPFEDFSVVGTGDKWFDAEPRADSPVTIRIIMK
                     DGKLYKNTL"
     gene            complement(2626..2913)
                     /locus_tag="AC16UZ_17020"
     CDS             complement(2626..2913)
                     /locus_tag="AC16UZ_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248443.1"
                     /translation="MRDEWVIESSYKLQLTRTVTLLPNVRVLIGPAADPGADTTVVLG
                     IRAGIAIWRSPQHPARTQQRTAANSITANVMVAARRRWHEVASGRRIANLG"
     gene            complement(2856..3125)
                     /locus_tag="AC16UZ_17025"
     CDS             complement(2856..3125)
                     /locus_tag="AC16UZ_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248444.1"
                     /translation="MKGMVTDEIWLGEGVYDANASSGEFDWSSVGEGEYLKHLEVGCT
                     PAFARRAKNTVQLTWWEKDRRKDAGTPCVTNGSSSPATNFSSPVP"
     gene            3245..4510
                     /locus_tag="AC16UZ_17030"
     CDS             3245..4510
                     /locus_tag="AC16UZ_17030"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR6248445.1"
                     /translation="MLIQGQKSGARGESPTVVELLFPFEIARDRGFDVEQGRANCISV
                     ANLVAHRFKAAVGRRSWRPPLAIWCHFRDGTGRRERYSRSYQVQPIWLLRNTYLIAYM
                     HALREHGVAPEPLLAKHGIVADFELIPDYMVSARLLHGYIIEAVSHGPPGAVSVAAGL
                     FNAAFQPNPFSDTAWKSVTLLEAIRRHNANVTRYSPENRFRFSVAKGEGHWEKQDRSP
                     LAETEIFCVANLIGHVRMSLGDGWSPPSVDVSVAEPSTLASLPLFEGVSVRAVPISAT
                     IHIPLSALALPSSSRDAGLSERALPDDFGDLDFVQSMRELIRSYVRVGELSFSNVALA
                     AGLSRRTLQRRLSDVGISYSGMVDQVRFEVARELLLESPELPVTQIGYELGYADPGSF
                     TRAFRRFAGTSPIAFRRASPSGMPTAEVA"
     gene            4626..5300
                     /locus_tag="AC16UZ_17035"
     CDS             4626..5300
                     /locus_tag="AC16UZ_17035"
                     /inference="COORDINATES: protein motif:HMM:NF019500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR6248446.1"
                     /translation="MRPLTSWRIVQALGKQRYLCLSLLAFLFALPLAGDEPGARLTTQ
                     LVLVFVFITGPLAVARTRATLWITGVFAIVVFVMGIGSVLGGGDWFLGTSALVGFVFF
                     GFLSWLVTRELLFDVKEVTAETLWAAVNVYVLIGLSFAFLYSVLALSSPDAFVGKFMS
                     EPLRDQLLGFVYFSFVTLTTLGYGDITPNNTFVGTLTYLEALVGQLYVAIMIARLVGL
                     YISSQK"
     gene            5427..5927
                     /locus_tag="AC16UZ_17040"
     CDS             5427..5927
                     /locus_tag="AC16UZ_17040"
                     /inference="COORDINATES: protein motif:HMM:NF024828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HIR6248447.1"
                     /translation="MNGQSLLAASLIAAVAWSAPAAAQTLASSMGIFIYPTQSQTSDQ
                     QSRDDYECFGFAKTQSGYDPMNPPQITAQAPDQGPSGARVAGAARGAAGGAVIGAIAG
                     DAGKGAAYGATLGAMGGGRADRQRRAQQSQQAEQSAQQQMANMQLTFKNAYGACIEAR
                     GYSVKY"
     gene            5946..6371
                     /locus_tag="AC16UZ_17045"
     CDS             5946..6371
                     /locus_tag="AC16UZ_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248448.1"
                     /translation="MRTAITSCFLLPLALSGVPAAAAQFDGKQDLLCSAFRMFECDMA
                     NGCTAVTPAELGASSSWIVNAKKKTITGTNPNAQPNDIDRMELLDGKLFMSGIQDGVP
                     EERDGVAWSVSINNPDGVMTIMVAGEGLGVVGLGSCVPR"
     gene            complement(6394..6996)
                     /locus_tag="AC16UZ_17050"
     CDS             complement(6394..6996)
                     /locus_tag="AC16UZ_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248449.1"
                     /translation="MRAILVTVGLLLVAGCATQTTVVRADFTQGEGAYVVGYSGDPVI
                     RTRMEEQLVNDLRAREMIAFASHTDIPDITASSRYELIAAARKRGVISVIVINQVSGD
                     ASDSVVQNPERISPTHPDLQAFYDYSLGRQPVVEPGQQVFAEVNLFVLDGDEASLFWS
                     GTTWSFQADGQGTAIRGISETIADQLQKVRDQARANPFTQ"
     gene            complement(7013..>7297)
                     /locus_tag="AC16UZ_17055"
     CDS             complement(7013..>7297)
                     /locus_tag="AC16UZ_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6248450.1"
                     /translation="IKNAIVLIEEIDQQIADGKEHLTAILDSTVSRLRPVVMAASTTI
                     LGLIPLLQDVFFVNMSITIMAGLGFATFLTLLFVPVLYSLFFKVAYREDA"
BASE COUNT         1266 a         2225 c         2333 g         1473 t
ORIGIN      
        1 ggctccgtgg gcgcttcagt gaactccgcc ggtatgttgg ccagatgata tcgaacgccg
       61 tcccgctggg tcaggagata cagctcgtag agatcgcctc gggcaagata gttcatgagg
      121 gtctgaattg accttcccgc gatggcgatg gtctgtcggt tgacgctttg gtgggtgatg
      181 tcgatatggc cgttgcgaat gatgtagaga ttgggcgctt catcgacttc cagcctgtcc
      241 agcaccgcct cccagtccag ggccagcggg tacaggttga ccacggagct ggtgttgccg
      301 tctacgtgga gctcgtcata ggtctgaccc ccggcctcta cctcgacgac taccggcggg
      361 agtgctgcag gaatggacgc cgacgcgagc atgacgtcgc ggatgagctc tagcgcgttg
      421 ggtgagcccg aggcggcgat ccggccaatc agccaggtca ccgatcgtcc ggcgtcgaga
      481 ttggtggtgg tgatgagcag cgacctgccc cgctggtact cgtcggcaat ggcgtccatc
      541 acctcttcgt caacgaactc agctattcgg gctcgcagcg gagcggaatc ggtggctgcc
      601 tcaccggtca gcgcttgcag caggccccgg gactcgagga ggtcctcggt ggagaattcg
      661 gtgtagaccc gcttcaagac gtgatcgtac gccgggccga gaaaggcgaa cggcgcgatc
      721 agcgccccgg tgctgatacc ggtgaccacc gtgaactccg ggcgggtgcc tgagtccgtc
      781 cacccgttga gcaacccggc tccaaacgcg ccgttggctc ccccgcccga gatggccagg
      841 tagttgtggg ggttgccgaa actcgcgggg taacgcgaga gcagatcttc ctgggaccgc
      901 tccagccaag ccttcgcgta gggcggcgca tcatctgccc agtatcggat gccgggcatc
      961 ccgggaacct cggcagcgtc tgccagcgag ggaggaaccg ggttgcgttc cggtaccgag
     1021 gcgcacccgc cggcgatcag cacaaggcag agggttccgg tcaggcggga cctggtcagc
     1081 gcggtgttca tccgggggct cgcgacctcg agatctccgg gcgtgccacg ccgactggtc
     1141 cggcccgctg gacgagcttc ctggtcggcc tgacagccct cgtaagttgg ctagagagtg
     1201 ttcttgtaga gtttcccgtc cttcatgatg atgcggatgg taactgggct atcggcccgc
     1261 ggctccgcat caaaccactt gtcgcccgta ccgacgaccg agaaatcctc aaacgggttg
     1321 ccgtcgataa tcaggatgtc ggcataggca ccttcctcga tgatgccgag tttggcgtcg
     1381 gggtaggggt tcatgaaatc gccgctcaaa gcggcgatct cgccaccgac ggacgtcatc
     1441 gccaccaagg aggtatgggg tccgaagaac tcgttgttca ggtgcttctc gtaggcgatc
     1501 tggatgttgc acgcggcgac tgatccgacg caatcggtct ggaagccgcg tttgacggga
     1561 cacttcttca tgttgtcgat atagccagcg aacgttgccg tcgctgattt cgccttggcc
     1621 aagctcgacg ggacactcgc gaccgccggg atttcgagaa gaccgggatc gaacgccgtc
     1681 aggttggtgg tgatgtaggc gcttttcttc ttcatcagtt tggcgatctc gcagtcgaac
     1741 gcgaagccgt gctcgattga tttcacgcct gcattcagcg cgttcatgat cgcttccttc
     1801 ctgtaggaat gcgccatcac gtagctaccg tattcattgg cgacctgcac ggctgccttg
     1861 atctcttcct gtgatccggc aagcaactgc caggggtcga aggccgaaac cacgccaccg
     1921 gactgcatgt acttcaactg cgtggcgccc atccggaaat tgttgcggcc gaacttctgg
     1981 acgtccgcaa cgtcgtcgac ctcctgcgcc agattcaggc gaccgaagtt ggtggattcg
     2041 ccgaccggtg aggtgtagtt cgcgaaatcc gcatgaccgc cccgggtacc gagaaacgct
     2101 gcggaggggt agaagcgcgg cccgacgatc tggcctgaat cgatcgcacg gcgcaggccg
     2161 gcgttcgcgc caccggcatc acgcaccgtg gtgaagccct gcatcaggta catttcggcc
     2221 atgcgcgtac cgtggatggc aaagtcctcc cacgtcgtat tggcttccat agccggcagg
     2281 ctcggaccca tcaacatcag gtgggcatgg ttttcgatga aacccggtag cagggtccga
     2341 ccgccgccat cgatcacctg ggcgtccgcg gcggcgatag gttggtccga gatcgttttg
     2401 attaggttgc cctctacgag cacgtgcatt ccgcgctgca ggtcctttgc ataaccgtca
     2461 aaaacgttca cgttggtgaa gagaacgcgt gccggctcat cctcggcccg ggatggcgac
     2521 gaccaggtca tcagcaaaat gagggttaga agcatgccca gaatattcac gttgtcctcc
     2581 ttgggtggtg ccatgttttg gagatggagt ttgcaatgca aacggtcatc ctaggttggc
     2641 gatccggcgc ccgcttgcca cttcgtgcca ccgtcgacgc gcagcgacca tgacgtttgc
     2701 cgtgatggag ttggcggctg tgcgttgctg tgttcgggct ggatgctgtg ggctgcgcca
     2761 gatggctatg cctgccctta tcccgagcac cacggtcgtg tctgcgccgg ggtcggctgc
     2821 ggggccgatc aggacccgca cgttgggtag cagcgtcacg gtacgggtga gctgaagttt
     2881 gtagctggac tcgatgaccc attcgtcacg cacggcgtcc cggcgtcttt ccgtcgatcc
     2941 ttttcccacc acgtgagctg gactgtgttc ttggctcgac gggcgaaggc cggtgtgcag
     3001 ccgacctcga ggtgcttgag gtactcgccc tcaccgacgg agctccagtc gaactcgccg
     3061 ctggacgcgt tggcgtcgta aaccccttcg cccagccaga tctcatcggt gaccatgccc
     3121 ttcatgacag cgccgatcgc tccgacaccc gtcgtctgcg ttcccggccg accaggtccc
     3181 agcttccctg ccagcggaat atcaggccgg ccccatgctt gttgccggag atggacctgt
     3241 ctgcatgctg atacagggcc aaaagagtgg tgctcgtggc gaatccccga cggtcgtaga
     3301 gctgcttttt ccattcgaaa tagcccgcga tcgcggcttc gatgtcgaac agggccgggc
     3361 gaattgcatc agcgtcgcga atctggttgc tcaccgattc aaggccgccg tcggtagacg
     3421 atcctggcgg cccccacttg ccatttggtg ccactttcgc gatggtaccg ggcggcgcga
     3481 gcggtactca aggtcctacc aagtgcaacc aatctggcta ctcaggaaca cgtatctcat
     3541 cgcttacatg catgcgttac gggagcatgg cgtggcgccg gagcccctgt tggcgaaaca
     3601 cgggatcgtg gcggatttcg agctgattcc cgactacatg gtctcggcgc gcctgctgca
     3661 cgggtacatc atcgaagcgg tgagtcatgg accgcccggt gccgtgagcg tcgccgcagg
     3721 gttgttcaac gctgcctttc agcccaaccc cttctctgac acggcctgga aatcggtcac
     3781 cctgcttgag gccattcgca ggcacaacgc caacgtgacg cgctacagcc cggagaaccg
     3841 ctttcggttt tcggtcgcaa agggcgaggg ccattgggaa aagcaggaca gatcgccgct
     3901 tgcggagacc gagattttct gtgtcgccaa cctcatcggt cacgtacgaa tgagcttggg
     3961 ggacgggtgg tcgcccccgt ccgtggacgt atcggtggcg gagccatcga ccctggcatc
     4021 cttgccgctg ttcgaaggcg tgtccgttcg cgcggtgccg atttctgcaa ctattcacat
     4081 ccccctttct gcgctggcat tacccagcag ttcccgggat gccggtttga gcgagcgggc
     4141 tctgccggat gactttggtg atctggactt cgttcaatcc atgcgggaac tgatcaggag
     4201 ttacgtgcgc gtgggcgagc tgagtttcag caacgttgcc ttggctgcag gcttgagtcg
     4261 gcgaacgctc cagcgacgcc tctccgatgt gggcatctcc tattccggca tggtcgacca
     4321 ggttagattc gaggttgcgc gcgaattgct cctagagtcg ccggagctac ccgtcactca
     4381 gattggctac gagctgggtt acgcagatcc aggatcgttt acccgcgcat ttcgtcgttt
     4441 cgccggcacc tcgcccatag cctttcggcg cgcttctccg tcgggcatgc cgaccgcgga
     4501 ggtggcttga ggcgcacggg gcaagctcga gacgctggcg tgattgctgg cgtgattgct
     4561 ggcgtgattg aaggaatctt gagcttccct ttgagcggag cgggctggct cggacgatga
     4621 ccgagatgcg gccacttacg tcctggcgaa tcgttcaggc ccttggcaag cagagatacc
     4681 tctgcctgtc gctgctcgcg ttcctgttcg cgctgccact cgccggggac gaacccggcg
     4741 cccgcctgac tacgcagctc gtgctggtgt tcgtgttcat taccgggccg ctggccgtcg
     4801 cccgcacccg cgccaccctc tggatcacgg gcgtgttcgc catcgtcgtg tttgtcatgg
     4861 ggattggttc cgtcctgggc ggaggcgatt ggttcctggg cacgtccgca ctggtcggct
     4921 tcgtcttctt tgggttcctg tcctggctgg tgacccgcga actgctgttc gacgtcaaag
     4981 aggtgacggc ggagaccttg tgggcggcag tgaacgtcta cgttctgatc gggctgagct
     5041 tcgccttcct ctactcggtg ctggcgctct cgagccccga tgcgtttgtg gggaagttca
     5101 tgtccgagcc gctgcgggac cagttgctgg ggttcgtcta cttcagcttc gtgaccctca
     5161 cgaccctcgg gtacggggat atcacgccga ataatacctt tgtcggcacg ctgacttacc
     5221 tggaggcgtt ggtcggccag ctgtacgtgg cgatcatgat cgctcggctg gtggggctgt
     5281 acatctcatc gcagaagtag cttccgggaa cgggtaggtc ggagcctcat cggaaactcg
     5341 cgcttgcagt gggcgttgca gcgatcgtat aaaggcgggc ccacggggta gggctcatgc
     5401 ccactccagg attcaaggag gactaaatga acggtcaatc cctactggcg gcttcgctca
     5461 tcgccgctgt ggcctggtct gcgccagcag cggcccagac gctcgcatct tccatgggca
     5521 tcttcatcta cccgacccag agccagacgt cggaccagca atcccgggac gattacgaat
     5581 gcttcggctt tgccaagacc cagagcggtt acgaccccat gaatcctcca cagatcacgg
     5641 cgcaggcgcc ggatcagggc ccgtccgggg cccgggttgc cggggcagcg cggggtgctg
     5701 ccggcggcgc cgtcatcggt gccatcgccg gcgatgcggg caagggcgca gcctacggcg
     5761 ccacgctcgg agccatgggc ggcggccgag ccgaccggca gcggcgggcc cagcaatccc
     5821 agcaggcgga acagtccgcg cagcagcaga tggccaacat gcagctcacc ttcaagaacg
     5881 cctacggcgc ctgcatcgag gcccggggct actccgtcaa gtattaaggg aaaggagaag
     5941 aacgaatgcg gacagcgatt acttcctgtt tcctcctgcc gttggccctc tcaggcgtcc
     6001 cggctgcggc agctcagttc gacggcaagc aggatctgct ctgctcggcg tttcgcatgt
     6061 tcgagtgcga catggccaat ggctgcacgg cggtcacccc cgccgagctt ggcgcttcga
     6121 gttcctggat cgtcaatgcc aagaagaaaa ccatcacggg caccaacccc aacgcccagc
     6181 ccaacgacat cgaccgcatg gagctgctcg acgggaagct cttcatgtcg ggcattcagg
     6241 acggggtgcc ggaggagcgt gacggcgtgg catggagcgt ctccatcaac aaccctgatg
     6301 gcgtgatgac catcatggtg gcgggtgaag gcctcggcgt ggtcggactg ggaagctgcg
     6361 tcccgcggta gacgccgaga ccacgggtcc gcctcactgg gtgaagggat ttgcccgtgc
     6421 ctgatcccgc accttctgca gctgatcggc gatggtttcg ctgatgccgc gaatggcggt
     6481 gccctgaccg tcggcctgga aagaccaggt ggtcccgctc cagaacaggc tggcctcatc
     6541 cccgtccagg acgaacaggt tgacctcggc aaaaacctgc tggcccggct cgacgaccgg
     6601 ctgccggcca agggaatagt cgtagaatgc ctgcaggtcc ggatgcgtgg gtgagatccg
     6661 ctcggggttc tggacgacgc tgtcggaagc gtcgcctgag acctggttga tcacgatgac
     6721 gctgatcaca ccacgcttgc gggctgccgc gatgagctcg tagcggctgc tcgcggtaat
     6781 gtccggaatg tcggtgtggc tggcaaacgc gatcatctcc cgtgccctga gatcgttgac
     6841 cagctgctct tccatgcgcg tccggatcac cggatccccg ctgtacccca cgacgtaggc
     6901 gccctcgccc tgggtgaaat cggcccgaac caccgtggtc tgggtcgcgc agccggccac
     6961 cagaagcagc cccacggtga ccagaattgc gcgcatgttt cctccagcga tctcaagcgt
     7021 cttcacggta agcgaccttg aagaacagcg agtacagcac gggtacgaag agcagtgtca
     7081 ggaacgtcgc gaacccgagc ccggccatga tggtgatgga catgttgacg aagaaaacgt
     7141 cctgcaggag cgggatcagg ccgaggatgg tcgtggacgc cgccatcacc accggacgca
     7201 gacggctcac cgtggagtcc aggatggcgg tcaggtgctc tttgccgtct gcgatctgct
     7261 ggtcgatctc ctcgatcagc acgatggcgt tcttgat
//
LOCUS       DBNHOX010000785         2251 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1284882, whole genome shotgun sequence.
ACCESSION   DBNHOX010000785 DBNHOX010000000
VERSION     DBNHOX010000785.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1284882"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="AC16UZ_17060"
     CDS             <1..562
                     /locus_tag="AC16UZ_17060"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF020341.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR6248451.1"
                     /translation="HVHPEWIEQGYAYYRAVWVECAELLDHFGWKWWKKQQPDLGQVK
                     LEVVDIWHFGLSDLIRAGTLDTAAPVLLEVSASEPTDLEGFRLAVEALAADTLSSRAF
                     AMAPFAAVLESLPMSFAELFEMYVGKNVLNNFRQDHGYKTGEYQKLWQGREDNEHLID
                     ILGELDCPAESVPAELYRALQARYPV"
     gene            complement(550..1059)
                     /locus_tag="AC16UZ_17065"
     CDS             complement(550..1059)
                     /locus_tag="AC16UZ_17065"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HIR6248452.1"
                     /translation="MTGIEVVEGDITTQSVDAIVNAANESLLGGGGVDGAIHRAAGPG
                     LKAECRRLGGCPTGEARITGGYDLPAAHVIHTVGPIWRGGDAGEAALLTACYRNSLDL
                     ARRHHIRSIAFPAISTGAFGYPLAEATAIAVATVGSFIATTPLLARFVCFDARTARAY
                     REERAAQTG"
     gene            1162..2016
                     /locus_tag="AC16UZ_17070"
     CDS             1162..2016
                     /locus_tag="AC16UZ_17070"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HIR6248453.1"
                     /translation="MTLLASSIARLDADARWALRGHELFFASLAVGEAQRAEGAAAEL
                     LASVEHGARALGVGKIGIDRPQDEALVAWYQQRGYYPGASPRGYLGEGRGEGPALLAK
                     DLNAAREMEVFFRGWAPELIGTLLFVIDGSRVLLIDKKTGHGAGKINAPGGKLEPGES
                     PVACAVREVREEIGITVAAPRLHAELAFADLRGSQWFGYVFVARAYRGEPVETAEARP
                     SWHPLNAIPYGRMWEDDRVWLPRVVAGERVRGRFLFRAGRLLAHALEGLGEVDGSEAE
                     HRGGCHGR"
     gene            2006..>2251
                     /locus_tag="AC16UZ_17075"
     CDS             2006..>2251
                     /locus_tag="AC16UZ_17075"
                     /inference="COORDINATES: protein motif:HMM:NF023949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HIR6248454.1"
                     /translation="MAADLNWLDSCRRLRAIAQTGLAYSRDPYDRDRFQELRLIAERM
                     LAWLSDAHPDRVAEVFLPERGYPTPKIDVRAGVFRGDS"
BASE COUNT          351 a          700 c          810 g          390 t
ORIGIN      
        1 ccatgtgcac ccggaatgga tcgagcaggg ttatgcctat taccgggccg tttgggtgga
       61 gtgcgccgag ctgctcgacc acttcggatg gaaatggtgg aagaagcagc agcccgatct
      121 gggccaggtg aaactcgagg tggtggacat ctggcacttc ggcctgagcg atctgattcg
      181 ggcgggcacc ctggatacgg cagcgccggt gctgctggaa gtatcggcga gcgagccgac
      241 ggatctcgag gggttccgcc tggcggtgga ggcgctcgcg gccgacacgc tttccagccg
      301 tgctttcgcc atggcgccgt ttgccgcggt gctcgaaagc ctgccgatgt ccttcgcgga
      361 gcttttcgag atgtacgtcg gcaagaacgt gctcaacaac ttccggcagg atcacggcta
      421 caagaccggg gagtatcaaa aactctggca gggccgggag gacaacgagc acctgatcga
      481 cattctgggt gagctggatt gccccgcgga gtcggttcct gctgagctct atcgagctct
      541 gcaagcccgc taccccgtct gagcggcgcg ctcctcgcgg taggcgcgag ccgttcgggc
      601 gtcgaagcag acgaaccggg caagcagggg tgtggtggcg atgaagctgc cgaccgtggc
      661 gacggcgatg gcggtggctt ccgccagcgg gtagccgaac gcgccggtgc tgatggccgg
      721 gaaggcgatg ctgcggatgt ggtggcgcct ggccagatcc agggaatttc ggtagcaggc
      781 ggtcagcagc gccgcctccc cggcgtcacc cccgcgccag atgggcccta ccgtgtgaat
      841 gacgtgggcg gcgggcaagt cgtagcctcc cgtgattcgt gcttctccgg tggggcatcc
      901 gccgagcctg cggcattccg ccttcagacc ggggccggcg gcccggtgaa tggcaccgtc
      961 aacgccgccc ccgccgagca gagactcgtt ggccgcgttg acgatcgcgt caacggactg
     1021 agtggtgatg tcgccttcca cgacctctat cccggtcatg tcgatgccgc ccgaagctgt
     1081 cgcccttcgc ccgaagggta gcccccaccc gtccccgggt aaaattgcca tcttccggcg
     1141 gcctgtcgac aatggcgggc catgacgctt ctggccagct ccatcgcacg gctcgacgcg
     1201 gacgcccgtt gggcgctgcg cggccacgag ctcttcttcg cctcgttggc ggtcggcgag
     1261 gcgcagcggg cggaaggcgc tgccgccgag ctgctggctt cggtggagca cggtgctcgg
     1321 gctctgggtg tgggcaagat aggaatcgac cggccgcaag acgaggcgct cgtcgcctgg
     1381 tatcagcagc gcggctacta cccaggggcc tccccacggg ggtacctcgg ggagggtcgg
     1441 ggcgaaggcc ccgctctcct agcaaaggat ctgaacgctg cccgtgagat ggaggtcttc
     1501 tttcgcgggt gggcgcctga gctcatcggg accctgctct tcgtcatcga tggctcgcgg
     1561 gtgctgctca tcgataagaa aacgggtcac ggggcgggca agatcaacgc gccgggcggc
     1621 aagctggagc cgggagaaag ccctgtggcc tgcgcagtcc gcgaagttcg cgaagagatc
     1681 ggcatcacgg ttgcagcgcc gcggctccac gccgaactcg cgttcgccga tctgcgcggc
     1741 agccagtggt tcggctacgt tttcgtcgcg cgcgcgtacc ggggagagcc ggtggagaca
     1801 gccgaggcca ggccgagctg gcatccgctg aacgcgattc cctatggccg gatgtgggag
     1861 gacgatcggg tctggttgcc gcgggtggtt gccggcgagc gcgttcgtgg ccggttcctc
     1921 ttccgcgctg gtcggttgct cgcccacgcg ctggaaggcc tcggtgaagt ggatggcagc
     1981 gaagccgagc accggggagg ctgccatggc cgctgatctg aactggctgg acagctgccg
     2041 gcgcttgaga gccattgccc agacgggact cgcttacagt cgggatccct acgatcggga
     2101 tcgatttcag gagctgcgcc tgatcgccga gcggatgctg gcctggctgt cggatgcgca
     2161 ccccgatcgc gtcgctgaag tattcctgcc ggaacgcggc tatcccacgc cgaagatcga
     2221 cgtgcgcgcc ggcgtgttcc gcggcgacag c
//
LOCUS       DBNHOX010000786         2046 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_64203, whole genome shotgun sequence.
ACCESSION   DBNHOX010000786 DBNHOX010000000
VERSION     DBNHOX010000786.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_64203"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="AC16UZ_17080"
     CDS             <1..804
                     /locus_tag="AC16UZ_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6248455.1"
                     /translation="YVDEVREVAIIWLLVWFALRFIRFMEVHLADSSYREEPVDETTA
                     AAVGKLLRAAAIITGVLMAMQEFGFSIAGVLAFGGVGGIAVGFAARDLLANFFGALMI
                     FLDRPFSVGDWVRSPDREIEGTVEDIGWRLTRIRTFDQRPLYVPNSIFTNLAVENPSR
                     MRNRRIYETIGVRYEDVGVLRQIVADVESMLREHPDIDHGRTLMLNFVEFGPSSLDFF
                     VYTFTKTTVWAEFHAIKQDVLFRIADIIEEHGAEIAFPTQTLHVVQPES"
     gene            801..1373
                     /locus_tag="AC16UZ_17085"
     CDS             801..1373
                     /locus_tag="AC16UZ_17085"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF006605.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HIR6248456.1"
                     /translation="MTARVPTEILTAREAAELVVGAQEVQAAIDRTAVRLAVALEAAN
                     PLVLCLLNGGLPFTAELIKRFSFPLELSYLHATRYGQRTSGSQLEWLARPPVSVAGRH
                     VLLVDDIFDAGETLMQVVEWLRREGAVEVTTAVLVDKAVPRSGAARVDYAALCCPDRY
                     LFGCGMDFRGYWRNLPAIYALPRSLEDEAV"
     gene            1373..>2046
                     /locus_tag="AC16UZ_17090"
     CDS             1373..>2046
                     /locus_tag="AC16UZ_17090"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: protein motif:HMM:NF006599.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HIR6248457.1"
                     /translation="MRRLAVLVGSGALKLPFDVLVEDFRDTPYGEASSKPSRAAGRNF
                     IVHVLARHGVPHAVAPHLINYRANLHLLSELGVEEIVALNTVGGIAKHARNGRIVLPD
                     QIIDYTWGRAHSFAAEGDLKHVDFTEPYDAALRAELLAAARGQGLDVHDGGVYGCTQG
                     PRFESPAEIDRMDRDGCTVVGMTGMPEAGLARELGMRYASLCLVVNPAAGRGDAIDLD
                     HIAVIAR"
BASE COUNT          321 a          645 c          683 g          397 t
ORIGIN      
        1 tacgtggacg aggtgcgcga ggtcgccatc atctggctgc tcgtgtggtt cgccctccgg
       61 ttcattcgat tcatggaggt gcatctggcc gactcgtctt atcgggagga gccggtagac
      121 gaaacgaccg ctgccgccgt gggaaagctc ctgcgggccg ccgccatcat cacgggcgtt
      181 ctgatggcca tgcaggagtt cgggttcagc attgctggcg tcctcgcctt tggcggtgtc
      241 ggcggcatcg cggtgggttt cgccgcgcgg gacctgctgg ccaacttttt cggcgcgctc
      301 atgattttcc tggacaggcc gttctccgtt ggtgactggg tacgttcccc tgaccgtgag
      361 atcgagggca cggtggagga cataggctgg cggctgaccc ggatccggac attcgatcag
      421 cggccgctct acgtgccgaa ctcgatattc acgaatctgg cggtggaaaa cccgtcgcgg
      481 atgcgcaatc ggcgcatcta cgaaaccatc ggcgtgcggt acgaggacgt cggcgtgctg
      541 cggcagatcg tggccgacgt ggaatccatg ctccgggagc atccggacat cgaccacggc
      601 agaacgctga tgctcaactt cgtcgagttc gggccttcct ccctcgactt cttcgtctac
      661 acgttcacca agaccacggt ctgggccgag tttcacgcca tcaagcagga cgtgctcttt
      721 cgcatcgccg acatcatcga agagcacggc gcggagattg ccttccccac ccagacgctg
      781 cacgtggtgc agcccgagtc gtgactgctc gggtccccac tgagattctg accgcgcggg
      841 aggcggccga gctcgtcgtc ggagcgcagg aggtgcaggc cgcgattgat cggaccgccg
      901 tgcgcctcgc cgttgctctg gaggctgcca acccgctggt gctctgcctg ctcaacggcg
      961 ggctcccgtt caccgcagag ctcatcaagc gcttcagctt tccgctggag ctctcctacc
     1021 tgcacgcgac ccgctacggc cagcgtacca gcgggtccca gctggaatgg ctagcccgcc
     1081 ccccagtgag cgtcgccgga cgccacgttc tgctggtgga cgatattttc gatgcgggcg
     1141 agaccctcat gcaggtcgtg gagtggctgc gccgagaagg ggccgttgag gtcaccacgg
     1201 ccgttctagt ggacaaggcg gtcccgcgca gcggagcggc ccgcgtcgat tacgcggcgc
     1261 tgtgctgccc ggaccgctat ctcttcggtt gcggcatgga ttttcgcggc tactggcgaa
     1321 acctaccggc aatttacgcg ttgccgagat ccctcgagga tgaagcggtc tgatgagacg
     1381 tctggctgta ctcgtgggct ccggtgcgct gaagcttcca ttcgatgtgt tggtcgagga
     1441 cttccgcgac accccctacg gcgaggcctc ctccaagcct tcgcgcgctg ccggtcgaaa
     1501 cttcatcgtc catgtgctgg cgcgacacgg ggttccgcac gcggtcgccc cgcacctgat
     1561 caattatcgg gccaatctgc acctgctctc cgagctgggc gtcgaggaga tcgtcgcgct
     1621 gaataccgtc ggcgggatcg ccaagcacgc gcgcaacggg cgcatcgtgc tgcccgatca
     1681 gatcatcgac tacacgtggg gccgcgccca cagcttcgct gcagagggcg acctcaagca
     1741 cgtggatttc acggagccat atgatgctgc gctgagagcc gagctgctgg ctgctgcccg
     1801 ggggcagggg ctggacgtgc acgatggcgg cgtctatggg tgcactcagg gcccgcgttt
     1861 cgagtcgccg gctgagatcg atcggatgga ccgggacggt tgcacggtgg tcggcatgac
     1921 gggaatgccc gaagccgggc tcgcccggga gctgggaatg cgttacgcga gtttgtgcct
     1981 ggtggtgaac ccggctgccg gtcggggaga tgccatcgac ctggaccaca tcgcggtcat
     2041 tgcgcg
//
LOCUS       DBNHOX010000787         4656 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04936
            SRR25158342_k127_1477445, whole genome shotgun sequence.
ACCESSION   DBNHOX010000787 DBNHOX010000000
VERSION     DBNHOX010000787.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414344
            Sequence Read Archive: SRR25158342
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:54:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,422
            CDSs (total)                      :: 3,396
            Genes (coding)                    :: 3,378
            CDSs (with protein)               :: 3,378
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158342_k127_1477445"
                     /isolate="MAG04936"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.13 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..465)
                     /locus_tag="AC16UZ_17095"
     CDS             complement(55..465)
                     /locus_tag="AC16UZ_17095"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6248458.1"
                     /translation="MTVEGGCYCGAVRYKAEGDPVFSAQCHCRECQYLTGGAPNMFMA
                     VPRSGFEYTKGEPSAFTRTDIENPVTREFCGNCGTHLLTRANAMPDAVILKVGSMDDP
                     GQYPGPQVAIFCMDKQTFHTVPAGVPEFERLPGG"
     gene            complement(589..1449)
                     /locus_tag="AC16UZ_17100"
     CDS             complement(589..1449)
                     /locus_tag="AC16UZ_17100"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6248459.1"
                     /translation="MRFLTYRLEGRTAFGAVIDGGIVNLAERLPAFGSLRDLLAAGAL
                     EQARRVAEDADPDHRLQDVGFLPPIPDPQKICCIGVNYGNRNEEYADAAPAARYPSLF
                     LRTRESLVGHLEPIVRPPESEQLDYEGEIVIVIGKAGRRIPESQARAHVAGLTIMNEG
                     TIRDWLRHAKFNVTQGKNFERSGSVGPWLVSLDEIERFDDLHLTTKVNGELRQDDRTD
                     NLLFSFEYLISYLSTFMRLQPGDLISTGTPTGAGARFDPPRYLKPGDVVEVAVDGIGA
                     LRNIVQDEVA"
     gene            1559..1975
                     /locus_tag="AC16UZ_17105"
     CDS             1559..1975
                     /locus_tag="AC16UZ_17105"
                     /inference="COORDINATES: protein motif:HMM:NF014958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate isomerase"
                     /protein_id="HIR6248460.1"
                     /translation="MPHLIIEYSANIDEELMLDALMLRLRDSAVATGVFPLPGIRVRA
                     ARRDRFVVADGDPENAFVHLMVRIGHGRTPEVRRQVADRLFAVLCDHLREAATRRGLG
                     LSLEIQEIDPVGSLKRNNLHERLAGRAAGHVSVKVE"
     gene            1972..2607
                     /locus_tag="AC16UZ_17110"
     CDS             1972..2607
                     /locus_tag="AC16UZ_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6248461.1"
                     /translation="MKIGPVLICAFALAMAPFAGAQSVSAWDLVYPSNFNWEIRTHRV
                     RVANDLASRVSLLVAVVPRQTEEESARLRETEQTLEARGADASPRDRSRLYLSRAYQH
                     RRLLELLADTLDALQCVRSAEELPREVHCWTRAAVNLMDEETVDVALTVLRDARMVPR
                     DEDMPVKAQDPKVWYGEYGKGIVRHIVMPYLAGLAAPRTPAATEEEVSHDG"
     gene            2600..3217
                     /locus_tag="AC16UZ_17115"
     CDS             2600..3217
                     /locus_tag="AC16UZ_17115"
                     /inference="COORDINATES: protein motif:HMM:NF019339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6248462.1"
                     /translation="MVERAPPIMLTLLLSSTLAAASETVLFNESAAFECYQAALHDGG
                     EYDIESCSRALEYQALTTIDRAATHSNRGILYARIGDIRNALRDHNRAVKLAPEVGSI
                     YINRSNTLVRAKRYEGAMRDLEKAIEIADDSLAYAHYNRALLFQKLGDLQAARADAEQ
                     AAEIAPDSPAYRRFLAAFGETEGDPLLPDAEPGNPPEEPGGEGQD"
     gene            complement(3208..>4656)
                     /locus_tag="AC16UZ_17120"
     CDS             complement(3208..>4656)
                     /locus_tag="AC16UZ_17120"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6248463.1"
                     /translation="EYAPREDQGFFMAQVLAPEGTGFDRMQEHMGGVEAPLMQMQDEG
                     LISRVLVRVPSWGGTSPNTGMVIVSLEPWRERDATTREIIDRASTAWEEIPAVRIFAF
                     ERSGLSRGGGGRPVQFVIGGPNYETLAQWRDLLIARAMEYPGMTRVDSDLKETQPQLI
                     VRVDKNRAAALGISVQNVGRTLAAMMSERRVTTFVQDGEEYDVILQAKEDQRATAADL
                     QNIYVRSESTGRLVPLANLIEVEETAGAGRLNRYNRLRAVTIEAGLAPGYALADALEF
                     LETVVREELPESAQVDYRGESLEFKEASGSLLFTFAIALLIVYLVLAAQFESFVHPLV
                     ILLTVPMAIAGALLGLKLTGGTLNIYSQIGIVMLIGIAAKNGVLIVEFINQLRDAGRS
                     FDDAIVEAAGIRLRPVIMTTISTVMGSTPLILATGAGSTSRSMLGVVIFSGVSLATLL
                     TLFIVPAFYHLLARRTGSPEETARRLKGLIQS"
BASE COUNT          802 a         1489 c         1532 g          833 t
ORIGIN      
        1 gctgaatctc tgctgtgccc gcgccgtaaa gcggcgcggg cgctccggcc tggatcagcc
       61 cccgggcagc cgttcgaact caggcacccc ggcaggaacg gtgtggaacg tctgcttgtc
      121 catgcagaag atggccacct gaggccccgg atactggccg gggtcatcca tggagccgac
      181 cttcaggatc accgcatccg gcatcgcgtt ggcccgcgtc agcaggtggg ttccgcagtt
      241 gccgcagaac tcccgggtga cgggattttc gatgtccgtg cgggtaaacg ccgacggttc
      301 gcccttggtg tactcgaatc cggacctggg aacggccatg aacatgtttg gcgcgccgcc
      361 ggtgaggtac tggcactcac ggcaatggca ttgcgcgctg aagaccgggt cgccctccgc
      421 tttgtagcgc acggctccgc agtaacatcc gccttccacc gtcatgggac ctctcctgtg
      481 attgattggc ttcccttacg agtgtaacgc gcccggcgga taagtgcctc ctccaccagc
      541 ctggcagggc cgagacctct gggcctctgc ggacgccgag ggctgaaact acgctacctc
      601 atcctgcacg atgtttctca gcgcgccgat gccgtccacc gctacctcga ccacgtcacc
      661 gggctttaga tagcgtggcg ggtcgaatct cgctcctgcg cctgttgggg ttcccgtgga
      721 gatcaggtcg cccggctgca gccgcatgaa cgttgacagg tagctgatca gatactcgaa
      781 ggagaagagc aggttgtcgg tgcggtcgtc ctggcgtagc tcgccgttga ccttggtggt
      841 caggtgcaga tcgtcgaacc gttcgatctc gtcgagggag acgagccacg ggccgacgga
      901 tccggagcgt tcgaaattct ttccctgagt aacgttgaac ttggcgtgtc tcagccaatc
      961 gcggatcgtt ccctcgttca tgatggtcaa gccggccacg tgagcgcgcg cctgactctc
     1021 cggtatgcgt cggcccgctt tgccgatgac gatgacgatc tctccctcgt aatcgagctg
     1081 ctcggactcc ggcggtcgca cgatcggttc caggtgacct acgagggatt cccgcgtgcg
     1141 cagaaaaagg ctcgggtagc gggcggccgg agcggcgtcg gcatactcct cgttgcgatt
     1201 accgtagttc acgccgatgc agcagatctt ctgcgggtcg ggaatgggcg gcagaaagcc
     1261 aacgtcttgg agccgatggt cgggatcggc gtcctccgcc acccggcggg cctgttccag
     1321 cgcgccggct gcgagcaggt cgcgcaggga gccgaaggcc ggcagacgct ccgcgagatt
     1381 cacgatgccg ccgtcgatga ccgcgccgaa agcggtcctt ccctccaacc gatatgtcaa
     1441 aaatcgcacg cgctctcccc gtctggcgcc tatgagcata gaatgtcagc gattccctga
     1501 tccggagcgc tgtcatactt caccctgatt cctgcagtgt caacgcgagg ctctcccggt
     1561 gccccatctg atcatcgagt actcggcgaa catcgacgaa gagctgatgc ttgacgcgct
     1621 catgctccgg ctgcgtgaca gcgccgtcgc tacgggcgtg ttccccctgc caggcatccg
     1681 cgtgcgcgca gcccgacggg accgcttcgt cgttgccgac ggggacccgg aaaatgcctt
     1741 cgtgcacctc atggtcagga tcggtcatgg ccggacgccg gaggttcgcc ggcaggtcgc
     1801 ggatcgtctg ttcgcggtct tgtgtgatca cctcagagag gcggctaccc ggcgcgggct
     1861 ggggctgtcc ctggagatcc aggagattga cccggtggga agcctgaagc ggaacaacct
     1921 gcacgaacgg ctggccgggc gcgccgcagg ccacgtctcc gtcaaggtcg aatgaagatc
     1981 gggccggttc tgatctgcgc cttcgcgctg gcgatggccc ccttcgccgg cgcgcaaagc
     2041 gtcagcgcct gggatctggt ttaccccagc aatttcaact gggagatccg tactcaccgg
     2101 gtgcgggtgg ccaacgatct ggcctcccgc gtgtcgctgc tcgtggcggt ggtgccacgg
     2161 cagacggaag aagagtcggc gcgattgcgc gaaaccgagc agacgctgga ggcgcgcgga
     2221 gccgatgcgt ccccaaggga tcggagcagg ctctacctct cgcgcgccta ccagcatcgg
     2281 cggctgctgg agttgcttgc agatacgctg gatgcgctgc agtgcgttcg atcggcagaa
     2341 gagttgccca gggaggttca ctgctggacc cgggcggccg tcaatctcat ggacgaggag
     2401 accgtcgacg tggcgctcac ggtgctcaga gatgcccgca tggtgcctcg ggacgaggac
     2461 atgccggtga aggctcagga cccgaaggtc tggtacggcg agtacggcaa gggcatcgtg
     2521 cggcacatcg tgatgcccta tctggccggt ctcgcggcgc ctcgaacgcc ggcggccacg
     2581 gaagaggaag tcagccatga tggttgagcg tgcccccccg atcatgttga ctttgctgct
     2641 ctcatcgacg ctggcggctg cctcggagac ggtgctcttc aacgagtcgg ccgcgttcga
     2701 gtgctaccag gcggccctgc acgacggcgg cgagtatgac atcgagtcgt gcagccgggc
     2761 tctggagtat caggcgctga ccaccatcga cagggcggcc acccattcca accgcggcat
     2821 tctctacgcc cgcatcggcg acatccgcaa cgctcttcgg gaccacaacc gcgcggtgaa
     2881 gctcgccccg gaggtgggca gcatctacat caaccgttcc aacaccctgg tccgggcgaa
     2941 gcgctacgag ggcgccatgc gcgacctcga gaaggcgatc gagattgccg acgacagtct
     3001 ggcttacgcc cattacaacc gggcgctgct ctttcagaag ctcggtgatc ttcaggcggc
     3061 gagggcggat gccgagcaag ccgcggagat agcgccggac tcaccggcgt accggcgctt
     3121 ccttgctgcc ttcggcgaaa ccgagggcga cccgctcctc ccggatgccg agccaggcaa
     3181 tcctccggag gaaccgggcg gtgagggtca ggactgaatc aggcccttga gccggcgcgc
     3241 ggtttcctcg ggcgagcccg ttcgccgggc gagcagatga tagaaagccg gaacgatgaa
     3301 gagtgtcagc agcgttgcca gggagacgcc ggagaaaatg acgaccccga gcatgctgcg
     3361 gctcgtcgag ccggcaccgg tcgcgaggat cagcggcgtc gatcccatga ccgtggagat
     3421 ggtcgtcatg atgacggggc gcaggcgaat gcccgctgct tccacgatgg cgtcgtcgaa
     3481 agaacggcct gcatcgcgca gctgattgat gaactcgacg ataagaacgc cgttcttggc
     3541 ggcaatgccg atgagcatga cgatgccgat ctggctgtag atgttgagcg tgccgccggt
     3601 cagcttgagg ccgagcagcg cgccggcaat cgccatgggg acggtcagca ggatgaccag
     3661 cgggtggacg aagctctcga actgggcggc cagcaccaga tagacgatga gcagcgcgat
     3721 ggcgaacgtg aacagaagtg atccgctggc ttccttgaac tccagcgatt cgccccggta
     3781 gtccacctgg gcggattccg gcagctcctc acgcactacg gtctccagaa actccagagc
     3841 gtccgccagg gcgtagcctg gggccaaacc ggcctcgatg gtcacggcgc ggagccggtt
     3901 gtagcgattg aggcgcccag ctccggcggt ctcctccacc tcgatgaggt tggccagcgg
     3961 aaccagacgg ccggtgcttt ccgagcgaac gtagatgttc tgaagatctg cggccgtcgc
     4021 ccgctgatcc tccttggcct gcaggatcac gtcgtactct tcgccgtcct gcacgaaggt
     4081 cgtgaccctg cgttcgctca tcatggccgc aagggttctg cctacgttct gcacggagat
     4141 gcccagcgcc gccgcgcgat tcttgtcgac ccgcacgatc agctgcggct gggtttcctt
     4201 caggtcgctg tcgacgcgcg tcatgccggg gtactccatg gcgcgtgcga ttagaagatc
     4261 gcgccattgg gccagggtct cgtagttggg cccgccgatg acgaactgaa ccgggcggcc
     4321 accgccgcct cgggacagac cggagcgctc gaaggcgaag atccgcacgg cgggaatttc
     4381 ctcccacgct gtggaggccc ggtcgatgat ctcgcgtgtg gtggcgtccc gttcccgcca
     4441 cggctccagg gagacgatca ccatgccggt gttcgggctc gttccgcccc agctggggac
     4501 ccggaccagc actctcgaga tcaggccctc gtcctgcatc tgcatcaagg gcgcctcgac
     4561 gccgcccatg tgttcctgca tgcgatcgaa accggttccc tccggcgcca gcacctgagc
     4621 catgaagaag ccctggtcct cccggggagc gtattc
//
